LOCUS       DAELLV010000001        67474 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.001, whole genome shotgun sequence.
ACCESSION   DAELLV010000001 DAELLV010000000
VERSION     DAELLV010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 67474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67474
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.001"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            112..654
                     /locus_tag="KPD58_000001"
     CDS             112..654
                     /locus_tag="KPD58_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0263802.1"
                     /translation="MKKFIKVSFFILGIFTLFIAINKLVYRDMVSVKDYEINHLEEIV
                     FDFDSRRSRDVGTIVRFKISNNSKYSYKLNSAKMKFENCVENKKGMNKCSAYVNVDLF
                     NEKNSITERTLKYGIKPNNQGYVDFVIPKGLNFDYRYFNESGMSVEYKGEYIVNIPMA
                     KGLYLVVGKNNDTWNANIME"
     gene            733..1200
                     /locus_tag="KPD58_000002"
     CDS             733..1200
                     /locus_tag="KPD58_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HBG0263803.1"
                     /translation="MSISNVREYFKQFGKEDSILEFEQSSATVELAAEAAGVIPARIA
                     KTLSFKIGDDAILIVTAGDAKIDNKKYKAEFNCKAKMLTPEEVLEFTGHAIGGVCPFG
                     LKNSIKVYLDDSMKRFDTVFPACGSSNSAIELTCEEMEKFSKCEKWVDVCKNW"
     gene            1909..3045
                     /locus_tag="KPD58_000003"
     CDS             1909..3045
                     /locus_tag="KPD58_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HBG0263804.1"
                     /translation="MDLNSKKYQMLKELYVSFAENEVKPLATELDEEERFPYETVEKM
                     AKAGMMGIPYPKEYGGEGGDTVGYIMAVEELSRVCGTTGVILSAHTSLGSWPIYQYGN
                     EEQKQKFLRPLASGEKLGAFGLTEPNAGTDASGQQTTAVLDGDEYILNGSKIFITNAI
                     AGDIYVVMAMTDKSKGNKGISAFIVEKGTPGFSFGVKEKKMGIRGSATSELIFEDCRI
                     PKENLLGKEGQGFKIAMSTLDGGRIGIAAQALGLAQGALDETVKYVKERVQFGRPLSK
                     FQNTQFQLADMEVKVQAARHLVYQAAINKDLGKPYGVEAAMAKLFAAETAMEVTTKAV
                     QLHGGYGYTRDYPVERMMRDAKITEIYEGTSEVQRMVISGKLLK"
     gene            3104..3886
                     /locus_tag="KPD58_000004"
     CDS             3104..3886
                     /locus_tag="KPD58_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBG0263805.1"
                     /translation="MNIVVCIKQVPDTTEVKLDPNTGTLIRDGVPSIINPDDKAGLEE
                     AIKLKEEMGAHVTVITMGPPQADMALKEALAMGADRGILLTDRAFAGADTWATSSALA
                     GALKNIDFDIIIAGRQAIDGDTAQVGPQIAEHLNLPSITYAEEIKTEGEYVLVKRQFE
                     DCCHDLKVKMPCLITTLKDMNTPRYMKVGRIYDAFENDVVETWTVKDIEVDPSNLGLK
                     GSPTSVFKSFTKSVKPAGTIYNEDAKTSAGIIIDKLKEKYII"
     gene            3905..4915
                     /locus_tag="KPD58_000005"
     CDS             3905..4915
                     /locus_tag="KPD58_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBG0263806.1"
                     /translation="MGNVLVVIEQRENVIQTVSLELLGKATEIAKDYDTKVSALLLGS
                     KVEGLIDTLAHYGADEVIVVDDEALAVYTTEPYTKAAYEAIKAADPIVVLFGATSIGR
                     DLAPRVSARIHTGLTADCTGLAVAEDTKLLLMTRPAFGGNIMATIVCKDFRPQMSTVR
                     PGVMKKNEPDETKEAVINRFKVEFNDADKLVQVVQVIKEAKKQVKIEDAKILVSAGRG
                     MGGKENLDILYELAEIIGGEVSGSRATIDAGWLDKARQVGQTGKTVRPDLYIACGISG
                     AIQHIAGMEDAEFIVAINKNPEAPIFKYADVGIVGDVHKVLPELISQLSVAKEKGEVL
                     AN"
     gene            4934..5731
                     /locus_tag="KPD58_000006"
     CDS             4934..5731
                     /locus_tag="KPD58_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HBG0263807.1"
                     /translation="MSTSDVKVYENVAVEVDGNICTVKMNRPKALNAINSKTLEELYE
                     VFVDINNDETIDVVILTGEGKAFVAGADIAYMKDLDAVAAKDFSILGAKAFGEIENSK
                     KVVIAAVNGFALGGGCELAMACDIRIASAKAKFGQPEVTLGITPGYGGTQRLTRLVGM
                     AKAKELIFTGQVIKADEAEKIGLVNRVVEPDILIEEVEKLAKIIAKNAQLAVRYSKEA
                     IQLGAQTDINTGIDIESNLFGLCFSTKDQKEGMSAFVEKREANFIKG"
     gene            5829..6674
                     /locus_tag="KPD58_000007"
     CDS             5829..6674
                     /locus_tag="KPD58_000007"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HBG0263808.1"
                     /translation="MKLAVIGSGTMGSGIVQTFASCGHDVCLKSRTQGAIDKCLALLD
                     KNLTKLVTKGKMDEATKAEILSHVSSTTNYEDLKDMDLIIEASVEDMNIKKDVFKLLD
                     ELCKEDTILATNTSSLSITEIASSTKRPDKVIGMHFFNPVPMMKLVEVISGQLTSKVT
                     FDTVFELSKSINKVPVDVSESPGFVVNRILIPMINEAVGIYADGVASKEEIDEAMKLG
                     ANHPMGPLALGDLIGLDVVLAIMNVLYTEFGDTKYRPHPLLAKMVRANQLGRKTKIGF
                     YDYNK"
     gene            6761..7936
                     /locus_tag="KPD58_000008"
     CDS             6761..7936
                     /locus_tag="KPD58_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBG0263809.1"
                     /translation="MREVVIASAARTAVGSFGGAFKSVSAVELGVTAAKEAIKRANIT
                     PDMIDESLLGGVLTAGLGQNIARQIALGAGIPVEKPAMTINIVCGSGLRSVSMASQLI
                     ALGDADIMLVGGAENMSMSPYLVPSARYGARMGDAAFVDSMIKDGLSDIFNNYHMGIT
                     AENIAEQWNITREEQDELALASQNKAEKAQAEGKFDEEIVPVVIKGRKGDTVVDKDEY
                     IKPGTTMEKLAKLRPAFKKDGTVTAGNASGINDGAAMLVVMAKEKAEELGIEPLATIV
                     SYGTAGVDPKIMGYGPVPATKKALEAANMTIEDIDLVEANEAFAAQSVAVIRDLNIDM
                     NKVNVNGGAIAIGHPIGCSGARILTTLLYEMKRRDAKTGLATLCIGGGMGTTLIVKR"
     gene            8131..9033
                     /locus_tag="KPD58_000009"
     CDS             8131..9033
                     /locus_tag="KPD58_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBG0263810.1"
                     /translation="MFKKENQRYNLISYTNEEEMTLKEVLLDKLNFSVRSLSKMKREK
                     SVLVNGVYKKPSLKVYSGDLIEVKIDEEKANFEPQDLNLQIIYDDFDIIMVNKPPFMV
                     VHPTKSHYDKTIANGISYYIDNQKENVKIRFVNRLDMNTSGLVIVAKNAYAHHTLSTA
                     MSENKVEKKYITVVDGIIKENEGTIDEPIYRPTEDSIKRIIDERGQSSVTHYKVIERL
                     ENATVLEVSLETGRTHQIRVHMAHIGHGIIGDELYGYVDEELINRQALHAYKLEFEQP
                     RTKEKLKFKADIPEDMKELISKLR"
     gene            9047..10051
                     /locus_tag="KPD58_000010"
     CDS             9047..10051
                     /locus_tag="KPD58_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBG0263811.1"
                     /translation="MIIHKFIIHVLDKNSDVPILNDFEGKVNQEVDGFFQKAIKRIAK
                     DEDLRKGVFKDYNDNLIKNCCEQIIYDESTFLKNSKEIASYLFDVMKINAALESCDLA
                     ICLYTIKDEKSVAILKLDYKKLYTHSIEFVDDKFNIQMVSNEIGIPETLRQKQGALIS
                     LSGINDEFHLRLLDKDAEKEGSESKFVTEFLNAKKIDDDKYKTKVFKNTAENWITNAL
                     SNDIKQAEDVRSILNYTLKEKHEVDINDFVDNSIKDDELKDSFKEHMEEKGLDESFSI
                     DKKWVEKKLKKRSIKTDNGFDIKGNLTDFEDPMKYTVKQNQDGTIDIIIKNVTFYEEK
                     "
     gene            complement(10195..10422)
                     /gene="acpP"
                     /locus_tag="KPD58_000011"
     CDS             complement(10195..10422)
                     /gene="acpP"
                     /locus_tag="KPD58_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBG0263812.1"
                     /translation="MFEKIKEIIAEQLGVDNLDEITMEASLMDDLEADSLDAVEIIMA
                     LEDEFGIEIPDEEAENFKCIGDICKYIEENK"
     gene            10594..11019
                     /locus_tag="KPD58_000012"
     CDS             10594..11019
                     /locus_tag="KPD58_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0263813.1"
                     /translation="MKYVGLLLTSVGMFLLIAVNFYYNSITLDMQRIEDYVMETNLIL
                     EDVAEKESYVSNEKEDYISRLMHVKKGIENSKTSFLIERYKEYKIKSIESLIYTISEE
                     KKDYLDEVDRYNKLGEKEINKLINKNFLEVTYLSITTYI"
     gene            11067..11273
                     /locus_tag="KPD58_000013"
     CDS             11067..11273
                     /locus_tag="KPD58_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0263814.1"
                     /translation="MTKLEDNMERFFGGCEGFFGNKMLIIIVIVFLLLCTDILEDLLC
                     DDNIWIWVILIVLLLFNFDDGCCC"
     gene            11455..11634
                     /locus_tag="KPD58_000014"
     CDS             11455..11634
                     /locus_tag="KPD58_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0263815.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFGDSWAEFDIMAWIPF
                     LIVLLILCCCGGCLE"
     gene            11668..11868
                     /locus_tag="KPD58_000015"
     CDS             11668..11868
                     /locus_tag="KPD58_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0263816.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFEDCWRDIDIMSWIPF
                     LILLLITCSCGGFFDGEVGCGC"
     gene            12121..13137
                     /gene="ccpA"
                     /locus_tag="KPD58_000016"
     CDS             12121..13137
                     /gene="ccpA"
                     /locus_tag="KPD58_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator CcpA"
                     /protein_id="HBG0263817.1"
                     /translation="MKGNITIKDVAKQAGVSISTVSRVINDSKPVTDEVKQKVLEVIK
                     ETGYIPNPLARSLVTKKSQLIGVIVPEVSDSFVNEVLNGIEEVAKMYDYDILLANTYS
                     DKEQELKSINLLRAKQVEGIVMISWIVEQEHINYIQNCGIPATYISKTARNYDIYTVS
                     TSNEEATFDMTEHLIKKGHEKIAFIMTSKDDTVLEMERLAGYEKALSNNNIELDKSLI
                     KYGGTDYESGYNSMKELLDDGIIPHAAFVTGDEAAIGAINAICDAGYKVPEDISVAGF
                     NDVKIARMYRPKLTTVYQPLYDMGAVAIRMVIKLINKELIENKKIELPYRIVDRESVT
                     ERKK"
     gene            complement(13260..13658)
                     /locus_tag="KPD58_000017"
     CDS             complement(13260..13658)
                     /locus_tag="KPD58_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0263818.1"
                     /translation="MKKLDTESLEKMAKQKNIDKDKIEKMADSYKGKSENELMEELIK
                     IGKNLDGRDEVVSKFKAFLDENQRKKLDNIMEKISDAENQRDTKPAKTKKAKPTKGKS
                     NSPAPKNTPGSQQKKSKSLFKKTKKSNPHE"
     gene            13870..14775
                     /locus_tag="KPD58_000018"
     CDS             13870..14775
                     /locus_tag="KPD58_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0263819.1"
                     /translation="MNNLLFLIGVIALSNGNISIFDDKNKSKGNNRVRKNKKNANDKL
                     SQSTHGNLRFDANDIKRGLRLMDLSEEDLEMGMEIITRTKKYMSRDERKILIKIESVL
                     DLVRGIKKLNNIDMVDTEDETDFFRKMDSDDKKNMMIKEIIDVFPEKRKNSVEKAIGM
                     KKKIDLFAELFLPDDFGEGGFSLSSLANINNLGSMNNLKLLGSLLRGDDSNNDDEDNN
                     DELNYEDEELDCEDKELDDDELTDEGEMEDNEDELVEDKNTGYENIENDEDIVYDEEL
                     YNINEQESEYNYNEKNTKTRAKKKR"
     gene            14890..15070
                     /gene="ssrS"
                     /locus_tag="KPD58_000019"
     ncRNA           14890..15070
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="KPD58_000019"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            15253..16470
                     /gene="cdeC"
                     /locus_tag="KPD58_000020"
     CDS             15253..16470
                     /gene="cdeC"
                     /locus_tag="KPD58_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeC"
                     /protein_id="HBG0263820.1"
                     /translation="MQDYKKNKRRMMNQPMSTMNEEEVYTDEINSEDMRGFKKSHHHN
                     GCNTDNKCECHDDCNPCNPCNPCKPNPCNPCKPNPCDDNCGCHDNCKCDCEPCEMDSD
                     ECFENKCGPECCNPISPRNFSVSNAVPFAIEANRIFDTMQFQTFTDATGPNGEPLTFE
                     TEVVEVFGSVPSAGQASVTIEKICLSNDGIVIDTGMTTLEDFDLDPLGDIVGRNCETT
                     FEFAVCGERNSECCRQGKGKSVAYKQRGLTVAVRNLVLELRGRCGCTEFVALAFPAVR
                     AGGGCKRRVDYVEFTFNTLSAPICLPADGRAVTLRQEYQTNLTVDCIGKSILKLECNE
                     CCEPFYELIIPNDIDLVLCLQETVSTLISEQIVVLASPNPIQPRLVDTFSKVCDFSQC
                     GPNHGSGKPSCHR"
     gene            complement(16570..17892)
                     /locus_tag="KPD58_000021"
     CDS             complement(16570..17892)
                     /locus_tag="KPD58_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBG0263821.1"
                     /translation="MKVPYLVLSTVILFISNFIVRIFGFLYKIFLSRALGETGLGIYH
                     MIFNFLMICLAVTTTGIPTALSCLVAKRKALNDRHNTNALFISTLYISFFVALIISIV
                     ASFNSSFLSLKFLKDAKLNLFILAVCPAIVIITLSNVLRGYYYGIKNVKIPAIGQIIE
                     QIGKILFVFLLVMYINNKSMNCYIALLGISIGELSNIIFMLICLWRDSSFDNRYIISI
                     KDFYNSSMETLKMSIPITCNRMSNILLQSISSMMIPSRLALSGMTYQQSLSMYGIVSG
                     MVMPFIFLPFTVGSALIVNLIPTISQEMALRKRKSVIKKIKYSILLTLFVGILSSIFF
                     YFFGKDLCILVFKNTLAGEYLKAMFLVPLFMSLNQTLSGILHSIRKELASSINTITGM
                     LIQLIALYVFLPIPGLNIYAYIYTMTIVSIFTCLLHTIVLFKSLKSIR"
     gene            complement(17943..18737)
                     /locus_tag="KPD58_000022"
     CDS             complement(17943..18737)
                     /locus_tag="KPD58_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HBG0263822.1"
                     /translation="MFYDYHMHSSFSTDGKSTMEQMVKKSIELGLEEICFTDHVDYDV
                     YADDSFSIVYEDYFKSLETLQSKYKDKISIKKGIEFGVQTQLMDTYKKEAHQYPLDFI
                     ICSIHAIDTMDLYLGNYFKDKTQHEVYENYYLYLYNIVKNYKDYSVLGHLDLIKRYAP
                     YDTVLDDRLFSDIIEETLKQAIYDGKGIEINTSCYRYNLPDLTPSKYILQMYKDLGGE
                     IITTGSDSHHISQVACEFDYIYSLLKNMGFKYVSKFNKLKPEFIKL"
     gene            18847..20292
                     /locus_tag="KPD58_000023"
     CDS             18847..20292
                     /locus_tag="KPD58_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG0263823.1"
                     /translation="MEKYRIDLNEKKSTKYIQIFNHIKELIINKELVEHEKLPPIRKL
                     SNFLQVNNTTIVKVYELLENEGYVYKIIGSGTFVSSLNSYKSGIDNRKNKIIVQREDL
                     IHFDNGNPSNDMFPIDSFKNAVNMALSKDGSSIFEYDEGLGSEELREKMVEYLSDGNI
                     KTTKENIQIISGAQQGIDIVCKGLISYSDVVFMEEPSYNGAIEVFKSRGAKIISIPML
                     DDGIDIGILKLKLEKIKPRLIYIMPNFQNPTGISYSTYKKKKLIELAEEHDFYIIEDD
                     FISDFVFESKDNRTIRSYDDKNRVVYIKSFSKILMPGLRIGIVEMPNELLKKVLWAKY
                     SSDISTPGLIQKSMYYYMNNFDWKNYLSHIEKIYTDKYKLAKNLINDKLSGKLKVRKS
                     CGGINFFLELPRGYTSQAFTNFMLNKGVSMLPGTYFFDNLVDDRFFRINIARPSMEEL
                     EKGISIISDNIDEFFCEYKNKLDIKSNKLFY"
     gene            20321..20530
                     /locus_tag="KPD58_000024"
     CDS             20321..20530
                     /locus_tag="KPD58_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HBG0263824.1"
                     /translation="MFDKKKLDRINELAKKNKEGILSADEIKEREILRKEYLENFRAH
                     FRSRLDSVKVVSPEEYEQYMKNNKN"
     gene            20550..21944
                     /locus_tag="KPD58_000025"
     CDS             20550..21944
                     /locus_tag="KPD58_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBG0263825.1"
                     /translation="MNLKYEFKNAWDFERKENTIEQIMQYSRNYMEFLSKSKTERLSV
                     KEIIKLAKENNYISIDEAMEKGSISCGDKIYVINKEKAVALFVIGKNYIEKGMKIIGS
                     HIDSPRLDLKPNPLYQESNLGFFKTHYYGGIKKYQWTAIPLALHGIVILNDGTKVDIS
                     IGEEDSDPVFCVTDLLIHLAGDQMQKKLSEGISGEALNILIGNIPLEDEEKEPITANI
                     LKILNEKYNIVEEDLLSAEIEVVPAGKARDLGLDRSMVLGYGHDDRVCSYAAVKAILE
                     TEQPEFTSVALCVDKEEIGSKGNTGMHSKFFENTVAELIALEGDYCDIKVRRALANSK
                     VLSADVSAGYDPNFGEAYEKRNSAYMGNGVVLTKYTGSRGKSGCNDANAEFMSEVKRI
                     FNKGNVVWQTAELGKVDQGGGGTIAHILANQGAEVIDCGVGVLNMHAPHEIVSKVDIY
                     EMYKGYKAFFNINL"
     gene            22184..22369
                     /locus_tag="KPD58_000026"
                     /pseudo
     CDS             22184..22369
                     /locus_tag="KPD58_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896033.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            complement(22478..23644)
                     /locus_tag="KPD58_000027"
     CDS             complement(22478..23644)
                     /locus_tag="KPD58_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG0263826.1"
                     /translation="MKGGVRKRGKKWYYYFDAGIVDGKRKKVERVGGNTKKEAEKSLR
                     DAINEYENAGIVFDETNMSLSDYLNFWYKEYVLLNCKYNTQESYRNLIENHIEPRLGK
                     CKLKSINPAIIQEFLNNKSKETYTQNGEEKHYTKGVLKAIYVVLNAALKSAVYPYKLI
                     KENPVQYASIPKNVLKVKNESDNKTITLDEFNKILEIYPKNTNIYIPLLIGFHTGMRK
                     GEILGLCWDNVDLDNNIIKVRKNLIKRKVSEFELASPKTKTSIRDIKIGDTLSRILKE
                     EKLNQKKQKIKIGKWYKETEYDWVCRKKDGSFVNHNNIDAAIRTINKKLNINFNFHCL
                     RHTHATLLLENGANVKYIQQRLGHSQLSTTMDTYSHVTSKMESETIDILEGILQ"
     gene            complement(23782..23988)
                     /locus_tag="KPD58_000028"
     CDS             complement(23782..23988)
                     /locus_tag="KPD58_000028"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0263827.1"
                     /translation="MNLSFNKKKKFELSKEELELIENWLGNKYAGDMTIPAIVDFSLE
                     TDIEYEKIVVYLAEKVLESRDKKH"
     gene            complement(24047..24268)
                     /locus_tag="KPD58_000029"
     CDS             complement(24047..24268)
                     /locus_tag="KPD58_000029"
                     /inference="COORDINATES: protein motif:HMM:NF024835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0263828.1"
                     /translation="MLKELRKKKKLTQIELAKRVGCHRSQISRLENNENKDLTIPALI
                     ELEIALGLEEKYLVNYFADEYIKKRKLHK"
     gene            24552..24689
                     /locus_tag="KPD58_000030"
     CDS             24552..24689
                     /locus_tag="KPD58_000030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0263829.1"
                     /translation="MNKTKYYDILKLNLLMKKLKELDKNKINEYKIKVKEIHKINKKE
                     E"
     gene            complement(24726..25121)
                     /locus_tag="KPD58_000031"
     CDS             complement(24726..25121)
                     /locus_tag="KPD58_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0263830.1"
                     /translation="MNRIKELREEKGISLDKLSEDLHINKSTLSRIENGLREPKKSTI
                     EEYANYFDVSTDYLLGRTDVRNSLFINKNEKDYDAENFKTEKELIENMYLDEDMKEVF
                     NIFSELSPDAREKALKVAELFLLDEKNKK"
     gene            25300..25533
                     /locus_tag="KPD58_000032"
     CDS             25300..25533
                     /locus_tag="KPD58_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF739 family protein"
                     /protein_id="HBG0263831.1"
                     /translation="MYLNRLEGLMKENRHTQKNVADILGLSSYGFRLKLKGKNEFKAS
                     EIKKISKLYNVSADYFFSDEVAKIAIKEERGNK"
     gene            25533..26285
                     /locus_tag="KPD58_000033"
     CDS             25533..26285
                     /locus_tag="KPD58_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HBG0263832.1"
                     /translation="MKNLTIIKQNNQFLVESREVAELIEKKHDNLLRDIRGYKKILED
                     SSNLKSQDFFIESTYINTQNKIQPCYLLTKKGCDMVANKMTGEKGIIFTAIYVTKFEE
                     MERELKEQQPKLPTTYKEALQQLLIEVEEKEQLQLENQVMKPKADYFDALVERNLLTN
                     IRDTAKELGVKEKTFVLWLIEKKYCYRDLKGKIKPYSNKMQYFELKEFTTPYGHSDTQ
                     TLINPKGREAFRLLLIKDGLVKEKEYQITLLG"
     gene            26330..26584
                     /locus_tag="KPD58_000034"
     CDS             26330..26584
                     /locus_tag="KPD58_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0263833.1"
                     /translation="MSEDIFYGIKNTLDEIAEVQIKNKQGVLKEVGMLISGEDNSCIT
                     HCLDEDLIKFYIKEEAVLTIDKDSHLLCMLDALFYNFLDK"
     gene            26612..27055
                     /locus_tag="KPD58_000035"
     CDS             26612..27055
                     /locus_tag="KPD58_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0263834.1"
                     /translation="MKKLGNISNFNLDKQEDKSFNDLDNISICFSEGIRKVVEMNLNN
                     YKNKISKYLNETSKIELLEPKELTIVISKGYPDYLMSVEEASKRLKIDKVFGYELIKN
                     GLLKSVDIGATKVSSYELDDFITRNQGKNIKEMLREMKELREGVI"
     gene            27183..27371
                     /locus_tag="KPD58_000036"
     CDS             27183..27371
                     /locus_tag="KPD58_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0263835.1"
                     /translation="MKIIYKNKVYKVEQDKKLFRITYYDEQRGSKKFNKEKKIKRSVL
                     TRDIELVNLYLPAHLKIK"
     gene            complement(27506..27676)
                     /locus_tag="KPD58_000037"
     CDS             complement(27506..27676)
                     /locus_tag="KPD58_000037"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0263836.1"
                     /translation="MSNKDIYTREEDKRFTLRINKLLFEKIEQLAQKDKRSIGREIEF
                     ILEKYFEDNPLE"
     gene            27819..27977
                     /locus_tag="KPD58_000038"
     CDS             27819..27977
                     /locus_tag="KPD58_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HBG0263837.1"
                     /translation="MTNERVRFTFRLPAPLLEKIKNRASIEGSSMNSLILHILWDYIK
                     EIENKEVK"
     gene            27977..28204
                     /locus_tag="KPD58_000039"
     CDS             27977..28204
                     /locus_tag="KPD58_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0263838.1"
                     /translation="MVELVKEFDLQTIKVGNAVKVNCKRFGFEIDCIVVVATEKELNL
                     AYFDEGRGCMEYQALITEDIQDGDYEIKILS"
     gene            28302..28616
                     /locus_tag="KPD58_000040"
     CDS             28302..28616
                     /locus_tag="KPD58_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0263839.1"
                     /translation="MESLKRIKKMVQKQLVLAELEINKNSKLYEELENKDRGLIDDIH
                     MREYLREKVAWERVKYAIENILGGINLEIKSKEHEESEDYKIFQLILEELERDKPIDV
                     QI"
     gene            28700..29209
                     /locus_tag="KPD58_000041"
     CDS             28700..29209
                     /locus_tag="KPD58_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HBG0263840.1"
                     /translation="MSTLYELTTDLLEIEEGLTETTGNETEKLEEIKEIIKQEIQNKN
                     TRIVSVILNIDSDINSIDSEIKRLQELKRVKKNTLDRLKSNIKDCMELLGTKKVETVL
                     GNISIRKSAGSLVIEDEEKIPAIYKTVEQVVKVDKNIIKDFIKKGHEVEGCRIEYGTT
                     LTIPKAKKE"
     gene            29219..29827
                     /locus_tag="KPD58_000042"
     CDS             29219..29827
                     /locus_tag="KPD58_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HBG0263841.1"
                     /translation="METNNVYIKLVNIQSTLKAPKSQFNSFGKYNYRSCEDILEGLKP
                     ILKEEKALVILDDNIVQIGNRFYVEATATLIDAETGEKVSTKALAREDETKKGMDLAQ
                     VTGSVSSYARKYALNGLFCIDDTKDSDATNKHGNEQKKKEVNESELNTLYSLGESIEK
                     DKNRVDSEVYKKFGKLAVDLTKQEYEKVLNGYKSILEKQKQE"
     gene            29837..30718
                     /locus_tag="KPD58_000043"
     CDS             29837..30718
                     /locus_tag="KPD58_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBG0263842.1"
                     /translation="MGIIRVSKDKDNPYVVLNKTCLEDVKLSWQAKGLHSYLISKPDH
                     WKIYVNDLCKRSKNGRDATANILKELIENGYITRKPCRDSNTNKMLGGYDYEVYEIPL
                     ENPQKLKSRKTDFPETGFPGNRVSRKPENTEVVSNDFKVNNDITTIVINEQSNKDKTT
                     YIKKYFEKYIGVITPNNFIELMSYLDDGMEADVIIRAVDEAVGSGVKNYKYVKTILNN
                     WIEAGVKTVLELTEYQNEFERKKKSKQEKKQSNSKTVNTHNVNKNKFANFNQTFTQYE
                     EKELDEIIKKSQKEKFK"
     gene            30779..30952
                     /locus_tag="KPD58_000044"
     CDS             30779..30952
                     /locus_tag="KPD58_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HBG0263843.1"
                     /translation="MARIYAQRSGSLNEQDRLELLRLLGKAGYTVKVAREKQNSKTTY
                     TYFVEYTEEQEEK"
     gene            30968..31375
                     /locus_tag="KPD58_000045"
     CDS             30968..31375
                     /locus_tag="KPD58_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG0263844.1"
                     /translation="MNTITLVGRLVADAELKYLPNSGTPKITFSMAVDRRFKDKNGNK
                     ITDFIQCEQLGKHVENLVQYLVKGKPIYAVGELNIYNYKDENGCWKSITKVNVNALEL
                     LSSKNDNNAKQEYVPPGLDPQGFQAIDDDDIPF"
     gene            31449..31643
                     /locus_tag="KPD58_000046"
                     /pseudo
     CDS             31449..31643
                     /locus_tag="KPD58_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861747.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            31695..31952
                     /locus_tag="KPD58_000047"
     CDS             31695..31952
                     /locus_tag="KPD58_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0263845.1"
                     /translation="MIIIRSQDKTDLVKADWINVDKECVYAMFGDMDNFKKIGEYEDE
                     KRAIQVLDNIQRFIEGGTRTDSIDSYKVRSYRNRIFQMPVK"
     gene            31966..32331
                     /locus_tag="KPD58_000048"
     CDS             31966..32331
                     /locus_tag="KPD58_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0263846.1"
                     /translation="MLKVEKYFSGSLGTNIIDDDPTCRNYLALYCCVHGVRKNGELIF
                     PTCEKMLIEFNVDKNRKKKKKGSKVKLINAKTGEEKIFDSIDSAACFLRLQSQAVYQT
                     IKKKTKTRSGWKAEYIEEE"
     gene            32449..32784
                     /locus_tag="KPD58_000049"
     CDS             32449..32784
                     /locus_tag="KPD58_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0263847.1"
                     /translation="MNILASVILVIGSFIAGRVYEYRLNLNENDEADSKVLLDVFNEI
                     SVLREENKSLKEKLQEKELLFINRLIDFLHDKKICECCIYDCKIDDIEYDCEDGIKKW
                     LDSEELIFE"
     gene            32892..33965
                     /locus_tag="KPD58_000050"
     CDS             32892..33965
                     /locus_tag="KPD58_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HBG0263848.1"
                     /translation="MLTFLDLFAGIGGFRLGMEKAGHKCLGHCEYDKFANLSYNAMHK
                     PKEDEWFERDIREIRTENIPRADVWCFGFPCQDISVAGKQFGFRGERSSLFFTVTKLI
                     RELKEEDRPKYLLIENVKNLLSVNGGFDFLKVLVELDEIGYDAEWQVLNSKNFGVPQN
                     RERIFIVGHFRGRSTRKVFPIERKSRKNLEQLNNPTHSTNRIYDAVGIARCIRSQAGG
                     GGAKTGLYFIDLNKNSKVTINARCLKAKYNAGVTNRNCDNSGVLVNAVLTPDRVNKRQ
                     NGRRIKESGEPMFTLTAQDKHGILKNGDIRRLTPKECFRLQGFPDKYYERAASVCSDS
                     QLYKQAGNAVTANVVYEIAKRMG"
     gene            34055..34264
                     /locus_tag="KPD58_000051"
     CDS             34055..34264
                     /locus_tag="KPD58_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0263849.1"
                     /translation="MYEYILRWQIGLSLENRKIHYTYGSKEALRKKAKALAKDENIVL
                     ITIDKVDEVIKNTISEKIIERFKNL"
     gene            34276..35271
                     /locus_tag="KPD58_000052"
     CDS             34276..35271
                     /locus_tag="KPD58_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBG0263850.1"
                     /translation="MIIHKFIIHVLDKNSDTPILNDFEGRVSQDIEAFFQKKISKVSR
                     DNDIRTAVFNDYSNNLIKKCCEQIIYDESSFLNNSKEIAAYLFDVMKLNATLESCDLA
                     ICLYSQKDEKKVAILKLDYNKSYTHSIEFKDDKFNIQMSKNEINIQETKTVKIAALVG
                     LSGMNDEYHLRVLDKDAEKEEANSKFVTEFLNATKVKDDKYKTKMFKAFVDSYIAHLY
                     SDMKQGEDVRGMLLYMLREKQKLDINEFADKAIKDDLKDSFKDHIEEKGIESFNIDKK
                     WVEKNLKNRHIKTDTGFEIKGKMEDFEDFMKYGIRHNGNGTVDIVIKNVNLYNEK"
     gene            35280..36005
                     /locus_tag="KPD58_000053"
     CDS             35280..36005
                     /locus_tag="KPD58_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HBG0263851.1"
                     /translation="MKYVASFSGGKDSAAMLLLILEKRLLLDEIVFIDTGLEFKEIYD
                     IIDDFEKRINFKITRIKAEKTFEEYFYTVNKQGKRKGQIWGFPYTLGAWCNSRLKLAP
                     ANKYFNSIGEHKRYIGIAFDEPSRYRRLEKNCIAPLYEAKMTEKDCLKYLEKKGFYYD
                     IHHRFKRTGCYLCPKQSLDSLRTLRKYYPDLWAGMLKLDKNSPTTFRADGTTVHDLEK
                     RFRNEDIENERQISFFQTEKVSI"
     gene            36002..36175
                     /locus_tag="KPD58_000054"
     CDS             36002..36175
                     /locus_tag="KPD58_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0263852.1"
                     /translation="MTNKEMCKSNNLDEREVCKSFGKEICASCINDKGDCESKDCDIA
                     YENWLEKEIVNYV"
     gene            36168..36539
                     /locus_tag="KPD58_000055"
     CDS             36168..36539
                     /locus_tag="KPD58_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0263853.1"
                     /translation="MFSIYKVKLKTKRTLEQVRNQSADFEYSEEGLKNTLRYYNLIDG
                     LEVIVVKFKDEYSLAGCDEKDVEKIRDAYYILEQDEYFGCYINEYERFKKDWENGECG
                     GEIGMMFSDDEVEIIEKLREG"
     gene            36542..37009
                     /locus_tag="KPD58_000056"
     CDS             36542..37009
                     /locus_tag="KPD58_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0263854.1"
                     /translation="MNFKLISDIPDWTIEEQLAKIDEEVTELKEAILEGDKEHIMEEI
                     LDVCQSALTATQVLKIDDKLYEGINKHNRKLGYRGLSLHNIKLPYRIGGRIYIITEEN
                     IFYEAILLGVEIDKHDLSLKNYIVLAQTYDREFVGCFKEIYTSEELEAVYEGR"
     gene            37087..37428
                     /locus_tag="KPD58_000057"
     CDS             37087..37428
                     /locus_tag="KPD58_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HBG0263855.1"
                     /translation="MANIYCENYNCKNYFEDMCMLERIEINNFKECESYLEGKNELYK
                     LENGYTIHPKDLKMVKSKDYSVEVTHIPTGITVKCRSTNSILKNKNKCLEVLEEELTK
                     INSHLELEDLR"
     gene            37554..37961
                     /locus_tag="KPD58_000058"
     CDS             37554..37961
                     /locus_tag="KPD58_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBG0263856.1"
                     /translation="MKVFLVIDGEPVGKERPRFNLATKRTYTPQKTKDYEELIKWLYQ
                     SKVRHYFTGYIKMTLRCYYSIAKSNSKKIKEQKRNNVLRPNKKPDIDNVVKIIADSLN
                     EIAYKDDTQIVEVVASKYYSDKPRVEVILEDIS"
     gene            38024..38764
                     /locus_tag="KPD58_000059"
     CDS             38024..38764
                     /locus_tag="KPD58_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HBG0263857.1"
                     /translation="MNNLKLIENEGLIKVYTTDEDIKVVDGRELWEGLEVEKDFSDWI
                     KSNLENVDAAEGIDFSTLKGKTSEQGGRPAIEYILNLEIAKEICLVAGASPRANKELK
                     RNSKNYRKYLIAVEEKYKVSNNLTKTQLNQINDIVSNALCEMQTKHDAQLEQFKKESS
                     QYYRPTSKTKYDISSYIKDRLDIPRANEEFDLVKKRTLLILGADKWEDIPKDVLLKSL
                     NIIDESIRIIKSERKTKQISFFEKDNFC"
     gene            38868..39350
                     /locus_tag="KPD58_000060"
     CDS             38868..39350
                     /locus_tag="KPD58_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0263858.1"
                     /translation="MNKKTLFQEVEGRLYNYKKLESQIRIKDIYIKKLENEFCGCKAQ
                     SYEEKTGETYNINSSVENEVIKREEDLNRLKEDKKTLEIEKETIECALTSLNSFETEF
                     FNEMYMNNEKINMDYMSNAMHIDRSHCFRIRKRIVCKIMDMLYPKIKEFELPIFSWKA
                     "
     gene            39818..40015
                     /locus_tag="KPD58_000061"
     CDS             39818..40015
                     /locus_tag="KPD58_000061"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0263859.1"
                     /translation="MDDLRGKIIKELDEFNIDADDEFLDYGVEYVESFTGKSAISEDL
                     LIRGVVLMANYIVNMENKDLD"
     gene            40145..40828
                     /locus_tag="KPD58_000062"
     CDS             40145..40828
                     /locus_tag="KPD58_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0263860.1"
                     /translation="MQDVKEKVKQDYLKGMKQKEISAKYDISLNTLKSWIKRYNWASE
                     KKKGAPKNKRGAPIGNKNATGPPGNKNAEKFGFFSKYLPEETRELIQEISIKDKFDIL
                     WEQITIQYAAIIRAQKIMYVKDKEEMIKELKKHESTENGEKIEYEFQFAWDRQASFLN
                     AQSRAMSELRSLIKQYDEMIHKDWNLATEEQKTRVEKLKCEVDNLSKDDIGDDELKIS
                     VDYGDRNDS"
     gene            40806..42074
                     /locus_tag="KPD58_000063"
     CDS             40806..42074
                     /locus_tag="KPD58_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HBG0263861.1"
                     /translation="MVIEMIVRIKFNPSFKEANFTKKRYRAMKGSAGSGKSVNVAQDY
                     ILKLGDKKYQGANLLVVRKSEATHKYSTYAELTGAINRIYGKQADKYWKTTLNPLEIK
                     SKVTGNSIIFRGVNDAKQREKLKSINFSKGKLTWVWCEEATELMESDIDILDDRLRGI
                     LTNPNLYYQMTFTFNPVSATHWIKRKYFDYKNDDIFTHHSTYLQNRFIDEAYYRRMKM
                     REEQDPEGYKVYGLGEWGETGGAILKNYVIHEFPTEFEYFDNMRLSQDFGFNHANAIL
                     RIGFKDGELYICNEIYVHEMDTSEIITIANSKGLEKSLFMYCDSAEPDRIKMWKSAGY
                     KAKGVKKGPGSIKAQIDYLKQLRIHVHPSCINTIKEIQQWKWKQDERTGLYLDEPVEF
                     MDDAMAALRYSIDNKLKNNGVRILTPNGRR"
     gene            42079..43479
                     /locus_tag="KPD58_000064"
     CDS             42079..43479
                     /locus_tag="KPD58_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG0263862.1"
                     /translation="MELDVIKKLIEQTNSKHNNFVKKADEAEKYYKNQNDIIRDRSPN
                     NVGKVNTANNPLRNADNRIPFNWFGFLVNQKVSYLFTYPPTFDVGDDGINSKITDILG
                     DRYPKEAKTLGKNASICSKAWLHVWEDDNNDFQYANIDPRQIRAVYSSDLNRKLLAVL
                     REYKKTDDEGKEYVIYEYWTDECCYTYQNKDGNSNINGLEILNKFIEKNLDNKLETQT
                     NAYKHNFGEVPFIEFLNNDLEVRDLDNVKHLIDVYDKVYSGFVNDIEDIQEVIFVLTN
                     YGGADLTEFLKGLKEYKTIDLQSSGADDKSGLSTITINIPIEARDSLLKTTEKQIYVQ
                     GQGVDPKPENFANTSGVALKFLYTLLELKAGLMETEFRLGFAKLVRIICKHIGYSPKR
                     VLQTWTRNMIQNDLELADICSKSVGIVSEKTNLKNHPLVDNAEEEEKQIKKEKEYNQQ
                     EYNSIIPNNSGVINET"
     gene            43469..44494
                     /locus_tag="KPD58_000065"
                     /pseudo
     CDS             43469..44494
                     /locus_tag="KPD58_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
     gene            44811..45071
                     /locus_tag="KPD58_000066"
                     /pseudo
     CDS             44811..45071
                     /locus_tag="KPD58_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012100325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            45318..45590
                     /locus_tag="KPD58_000067"
     CDS             45318..45590
                     /locus_tag="KPD58_000067"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0263863.1"
                     /translation="MFKKKYIKKPSKISVRNIIAFIITVIGIALGVFIGINIIMAHVL
                     GIANMVDNNTFTCVRLVYNLVGVISGYLIGKAIYLIASLISYIIYE"
     gene            45641..45871
                     /locus_tag="KPD58_000068"
     CDS             45641..45871
                     /locus_tag="KPD58_000068"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0263864.1"
                     /translation="MKEYVIWFKSGNCVSGITDEYVADKLMKDFIEVDSDCRYLKGYL
                     DEDGTTVIDLSQIEAISINNCNENNNIGFSKS"
     gene            45916..46488
                     /locus_tag="KPD58_000069"
     CDS             45916..46488
                     /locus_tag="KPD58_000069"
                     /inference="COORDINATES: protein motif:HMM:NF018515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HBG0263865.1"
                     /translation="MKKGELIALGLSEEDAKKVEVESLKELDNYINKIEYEKVKEELK
                     ASKEAIEGFKDGMTKEQIEELKKGYETKLTAKDEEYQNKLKEKEQKEFDMALENELIK
                     LNVHSTKAAKAELDLEKIKYENGTFTGLKEQTDTWSTQKSFLIKTGKTKINYSPDNGN
                     ENTLSRAESIAKEKNEESSKNPYADAWSIK"
     gene            46504..46884
                     /locus_tag="KPD58_000070"
     CDS             46504..46884
                     /locus_tag="KPD58_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0263866.1"
                     /translation="MYFKEVNFDNTPEFLASQHYINFSKTAIDTDVVADENGKKYVLA
                     GSLLGESGKVVKITRGGSSDSYTYSLSENPVGIVFSTVDVTYGPQPVASMVEGYVITE
                     RLQGEYVKEAIDTIKTKLPNIKFM"
     gene            46896..47915
                     /locus_tag="KPD58_000071"
     CDS             46896..47915
                     /locus_tag="KPD58_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HBG0263867.1"
                     /translation="MARVEELLSVQELINYTKTRKLKETMGDLLFPTQKIEGLEIKMI
                     KGASNLPVSASVHAFDTEAEIASREGANLSIAELALVKRKIKLDEKDIIVLEEPRNSQ
                     EETQMINQIFNDVDNLVSSVNTRIEAMRMEVLTTGELNINENGVKASLKYGTPTNHKE
                     TKTWSSGTPDILGDIYNMTDKIVVDTGFTPTRSLTSKTILNTILRDEKLRKAIFGVNS
                     DKLLTLKELNVFLASQSLPPIFTYDERYRVQGKDGKYTTKRFLDENKFILMPDGKMGD
                     TFFGLTAEELELRKNPSIDISSVGNIIVEQYSTADPVAKWIKAVATVLPSFPYADQVF
                     MGTIN"
     gene            47926..48270
                     /locus_tag="KPD58_000072"
     CDS             47926..48270
                     /locus_tag="KPD58_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HBG0263868.1"
                     /translation="MEVERLKKLLGFSREDDSKDTILEFILEDVEEMVKNYCNVPTIP
                     EQLNSTILRMAIDMYKNESLGSEDIALGSISSISEGDTSVSYRSSASEFKESLLKDYK
                     SQLNRYRKLRWK"
     gene            48270..48617
                     /locus_tag="KPD58_000073"
     CDS             48270..48617
                     /locus_tag="KPD58_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0263869.1"
                     /translation="MMDKTRKAIEMLYRDKCTIVEYQPIKDPVTKRANNKEVVVLENQ
                     PCKLSYKNIVSATEGKVAKLEQTIKLFISPDIEIKAGSKLIINDKEYVRSGESAIYPN
                     HQEIILELFKDKA"
     gene            48617..49042
                     /locus_tag="KPD58_000074"
     CDS             48617..49042
                     /locus_tag="KPD58_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBG0263870.1"
                     /translation="MARWGSVDFREFKRVCKKMEKLTKIDLDKFCKDAARELAARLLG
                     KVIRRTPVDTGFLRQGWNGVAYARSLPVYKQGNNYIIEVVNPTEYASYVNFGHRTKDG
                     KGWVKGQHFLTISEMELQSQVDKIIEKKLLILLKGVFDA"
     gene            49035..49472
                     /locus_tag="KPD58_000075"
     CDS             49035..49472
                     /locus_tag="KPD58_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0263871.1"
                     /translation="MLNNIIDGISVKLDKSFGEKYTIYSEDVEQGINEPCFFIVPLNP
                     SKTPYPSGRELKKNSFDVHYFPRSEAKNFEINEIAEMLLEELEYIEIDGDLVRGTNMN
                     FEIIDNVLHFFVDYNYFTIKNNDTNKMDTVELFGGLKRGDNFE"
     gene            49465..49641
                     /locus_tag="KPD58_000076"
     CDS             49465..49641
                     /locus_tag="KPD58_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0263872.1"
                     /translation="MNKTLSKEDDYKFTKEQIVNSKKYVNRKDLLNAILKENELYSFS
                     EVEEIINKFMKGVS"
     gene            49642..50952
                     /locus_tag="KPD58_000077"
     CDS             49642..50952
                     /locus_tag="KPD58_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HBG0263873.1"
                     /translation="MALGGGTFVTQNKILPGSYINFISAKRATSSLSDRGIVAMPLEL
                     DWGIDEDVFQVTSDDFEKYSVKYFGYDYTHEKLKGLRDLFKNIRLGYFYKLNKGVKAS
                     CTIATAKYSGIRGNDLKVTVTTNIDDNTKFDVVTLLDNKKVDTQIAKVITDLEDNDYI
                     TWRKDATLEASAGLVFTGGTNGEAVTGAEYQAFLDKIESYSFNALGCLATTTEIKSLF
                     VEFTKRMRDRVGAKFQTVLYKKNDADYEGVVSVENKIKDTGLLESSLIYWTTGAIAGC
                     DINKSNTNKRYDGEFDVDVNYTQIHLEEALKTGKFIFHKVGDEVHVLEDINTFVSFTD
                     EKNDDFSSNQSVRVLDQIANDIATLFNTKYLGEVPNDKSGRISFWNDVVKHHEQLQNM
                     RAIEDFKADDVSVEPGSDKKTVVVSDAVKVISAMSKLYMTVSVS"
     gene            50974..51444
                     /locus_tag="KPD58_000078"
     CDS             50974..51444
                     /locus_tag="KPD58_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBG0263874.1"
                     /translation="MAQQIKARDTISASKAECFVTIKGKRYNFMQAINLEAKMEKNKS
                     EIPILGSTTKGNKSTGSKYSGNATFYYNTSIFRELLYEYKETGEDIYFDIQITNEDPT
                     SSVGRQTIILEDCNMDSGIIAKFDADGEYLDEDMDFTFENWKLVEKFNIANGME"
     gene            51497..51754
                     /locus_tag="KPD58_000079"
     CDS             51497..51754
                     /locus_tag="KPD58_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0263875.1"
                     /translation="MIKIKDKYEIKDSISFDYSNKRPLEERVSEMYKKAGKYLIDISD
                     KLATDTIDGSSLKPIIIKFEINEAGVATIEKQTKYLVMEVE"
     gene            51759..52199
                     /locus_tag="KPD58_000080"
     CDS             51759..52199
                     /locus_tag="KPD58_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG0263876.1"
                     /translation="MGDLNAFLSQNAIKVENRKYVASERFIGEDGKAIEWELKAIDSD
                     RDRQLRKDSTIRVPILNKKGKATGQYTSETDFNTYTLKLCVETIVFPDLHDAELQNSY
                     GVMGAEELLTTMLTPGEYTDLSSEVGEVNGFDRTFEDKVEEAKN"
     gene            52520..52681
                     /locus_tag="KPD58_000081"
     CDS             52520..52681
                     /locus_tag="KPD58_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0263877.1"
                     /translation="MDNFLLSILASLIASLIGYIVCRCIKNVKSHSTRGKSKSGWELG
                     FKIKFRKFK"
     gene            52830..53063
                     /locus_tag="KPD58_000082"
     CDS             52830..53063
                     /locus_tag="KPD58_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0263878.1"
                     /translation="MNKDIELTEKDLYCIARHIQVYVFKKDDEVIKKEDNPCCRCKHK
                     FKCYENSVCIYHCSVFQKLSKITGLKMGFGVKL"
     gene            53123..53650
                     /locus_tag="KPD58_000083"
     CDS             53123..53650
                     /locus_tag="KPD58_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HBG0263879.1"
                     /translation="MREGKRRRGCLFWFIIIIIFSGVVGAIAGNSTNNGSTEKQKEDL
                     TKYIGEEGNIGDLKLTVNSISKASEIPVASGYLAYTPDSGKYGIINLTIKNQTKESQS
                     FMLNSFTLIGPDNSKYVPSLLIDVGNKYITMDTVNPNLDVTGNIAFEIPKDLLVSDCK
                     LKYSGSEQEFILKEK"
     gene            53792..54352
                     /locus_tag="KPD58_000084"
     CDS             53792..54352
                     /locus_tag="KPD58_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG0263880.1"
                     /translation="MFCSNCGAEITGIGKFCSSCGVAVETEIIEDNNIKSNDLIVDAN
                     GIEINMTEIYRKYRKEKVNAIKNVMEISGLNIKEAKKIVDFSFEELKINFIDDTMSNS
                     EKEKIIQNQNRKNNIEKAQQESVACCPKCGSTSLTAQKKGFGIGKAMVGASLTGGIGL
                     VAGNLGAKKVRVTCLNCGKQFWAGKK"
     gene            54430..56718
                     /locus_tag="KPD58_000085"
     CDS             54430..56718
                     /locus_tag="KPD58_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBG0263881.1"
                     /translation="MATIQTSIRIFDGMTPAFRHMTTAMNIVLSSFEQLQRTSSNAVN
                     ANSIIRAREELARAEAGFDRLERQIRESDNQQRKLNEDINKGASSTDRLVGSAKKLAA
                     TYLGIRTLGGLGNLSDQMTSTNARLGMINDGQQSDAGLNKMIFQSAERSRASYLDTAQ
                     IVSRIGMNAGKAFSSTKEIVGFAEQLNKKFVIAGASTEEMNSALLQLTQGLSSGVLRG
                     EELNAVFESAPNIIQSIADYLDVDIGKIRGMASEGMLTADIVKNSLLAAAEQTNAEFE
                     KMPYTFSQIWTSIKNNAIMIFGVIQKKIEQSMSSKGFRTFIDNFINSLYVLGNVAYNI
                     FNEIISILGSPFFQAFVNAIIVGVSLIVQALGWIITQALNIANVFAQNWSIIAPIVLG
                     VAAAMLVYNNALLLSIANKVKDIALSAKSLVMSFAHIVAESYRAAALVATTIAQDGLN
                     AAMAACPITWILYGIIAIVVAFFVAIAIFNHFAGTSVSAIGVVAGAISVAASFIGNLF
                     IATGNLIIDIVALIYNTLAGFAEFFANFLDDPIGSVIRAVSGMANAVLGIIRSIASAF
                     DTVFGSNLADAVSGWQDKLQGWTDKVAGEAKIKVERMDPNKLHFDRFNYGKAWDAGYK
                     WGDKLETNIKDKFDISKMAEDAKKKLGLDDLWDKKYGLGDGFGSAGLNSPLNDAAKGA
                     KDTAGNTAKMAKTMDKSQEDLKYLRDIAEQETINRFTGVNIKIDMNNTNNISKDADVD
                     GIVNVLTEKLNDAMVVSAEGIV"
     gene            56734..57435
                     /locus_tag="KPD58_000086"
     CDS             56734..57435
                     /locus_tag="KPD58_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBG0263882.1"
                     /translation="MAYDFYLDGVQLPIPPPKLEIKVTNKNKTVDLINTGEVNILKKE
                     GLSEISFEAEFTHNKLPFYRGTFRDVQFFLSKLELLKTDCKPFQFIVSREMGGKVLFN
                     TNMKVSLEEYNIVEDADNGSDVKVAIKLKQYRDYSTKKLVPATPEKTNYGRTPTPVMK
                     PKEFRPDSSNKPNGKTYTVKAGDSLWSICQKQLGNGSLYKKVYELNKTMMDKANKGKK
                     VPKYTIYKGQVLRLV"
     gene            57428..59380
                     /locus_tag="KPD58_000087"
     CDS             57428..59380
                     /locus_tag="KPD58_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG0263883.1"
                     /translation="MSDELVLANDRDVRLVIAHWEDFYEPVVLDGITWEIERRGTPSK
                     LEFTIVMDDILQFCEGNSVRLYYKGIGIFYGYIFQKKRDKENHIKIVAYDQLRYFKNK
                     DTYVYSNKTASELVKMLAKDFNLKYNVIEDTKYKISRVEENKTLFDMVLTALDDTLRQ
                     KKEMYVLYDDFGRLTLKNVASMKLDTVMNNDVIEDFDYNSSIDSDTYTKIKLVRDNEE
                     SGKRDVYIAQDSTHIRSWGILQMFDTVDDNLSEAEIKQKCDILLKLYNKKTKSLSLKN
                     AIGDIRVRAGCLVPVFLNLGDIELQNYMLVEKVKHTFENNSHFMDLTLVDGDEFASYS
                     SSSYSSGNSNNKDEKKNGPAQSTTNKEDSDMINKLNKVFKGKLSNTGSIFVKYSNAYK
                     VNAALMAAISIHETGNGSSSLCKNKNNFFGMKGMSFGSVDEGIKRGISNLSRNYIHTG
                     RKTLESIRDKYAPLYDSPLNKDWVPGVGKFYKQITGSAYSSNSAGTGVGSNEEAEKNL
                     KDITYQVQGNNQNSNANNSKADKLISIAKSKLGCKYVWGAEGPNTFDCSGFTQWCYKQ
                     VGIKIPRTVATQSKAGKAVDLKDRSKWKAGDLLCRVGGGNSNHVMMYIGNGKMIHSPQ
                     TGDVVKIQSVDSYRKGKAYTHVRRFI"
     gene            59394..59654
                     /locus_tag="KPD58_000088"
     CDS             59394..59654
                     /locus_tag="KPD58_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HBG0263884.1"
                     /translation="MSQDLLQVIKKAAMDAVETSNPMMIAFGTIESLNPLVVYIEQKA
                     SFDEFFLIQTETFKRYTDKKIGDKLVLIRMQGGQQYLILDRM"
     gene            59659..60078
                     /locus_tag="KPD58_000089"
     CDS             59659..60078
                     /locus_tag="KPD58_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBG0263885.1"
                     /translation="MLPTDNIDYDIEDVSIINFDVRQEPSKTFKLHIEKNRVDGICDD
                     VEALKQTIFLILNTERYEHLIYSWNYGVELNDLIGEPISFVIPEIERRIKEALIQDDR
                     IETVDNFEFQNVKGKVHCKFMVHTKYGNIKAEKVVSV"
     gene            60079..61128
                     /locus_tag="KPD58_000090"
     CDS             60079..61128
                     /locus_tag="KPD58_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBG0263886.1"
                     /translation="MFELMTFENIIKRMLDSVPNTLDKREGSIIYNALAPVAVELTET
                     YIAMDELLDQTFVDTASYYYLEKRCKERGITPLPATNTIAKGVFNIDIPLDSRFNLGE
                     YNYIAIERISEKTYKMKCETAGPIFELGQLIPIEYIDGLETAELTEILINGEDEESED
                     SLRQRYYDSLNSQSFGGNMQNYKDEVNKIQDVGGVKVYPVWDGGGTVKLVIINSNFKV
                     PSEDLVNLVQEEIDPIGHQGQGLGLAPIGHRVTVEGVTSTTINISAEITYKSGYTWEN
                     IKTIAEEAVDDYLNELNMSWEDEENLIVRISQIETRLLSIDGVLDIANTMINSIASNL
                     NVDKDSIVLRGEVVG"
     gene            61121..61738
                     /locus_tag="KPD58_000091"
     CDS             61121..61738
                     /locus_tag="KPD58_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HBG0263887.1"
                     /translation="MDKEINLINYLPQILQDKEEYIKVFNVENKEIKTLHDKLKDLSN
                     DQFLEDLTPSGVKRWEKIMSINPKSNETLEDRRFRIFSKYISKLPYSERFLRNWLDNV
                     VGEGNYELTINNATYNIHLESDARNQDWFEEVHSFVSNIKPCNMTLDYTRVLISKDNY
                     MNFGITTLMGQEITIYPWSPPDIETYGEIDVLTGNGVGYQEITIF"
     gene            61750..62775
                     /locus_tag="KPD58_000092"
     CDS             61750..62775
                     /locus_tag="KPD58_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBG0263888.1"
                     /translation="MAIDKSYYTIITDVGKAKIANASVTGNKVGFVKIQLGDGGGSEY
                     TPTESQTALKNVVWEGNIGNTTTDETAPNCIILESLIPSSVGGFMIREIGYLDDENNL
                     IAISKYKECYKPSIEQGAVVDMKVKTVLIVSNVNNIELKIDPTIIFATLKDIQDLETK
                     IGTVNTKIDTTKTELTSNIETTKTELNTRIDTENEKQNIKIDQLIAGGSNVASTQTIT
                     IDDWVEDAENGFKATVTHSLLTQRIVVNIIDATTKENVVTNFKIIDDNSIEVRSETRS
                     ELNVYVINGNAETHFINATVDDNRVSEMTTYSSKKIEDRLVNLEEKVNGGLSSIATSV
                     NELITYC"
     gene            62792..64486
                     /locus_tag="KPD58_000093"
     CDS             62792..64486
                     /locus_tag="KPD58_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0263889.1"
                     /translation="MQTEWNFDAVSSAQEIILKPGKYKFECWGARGGALGTPFESGFY
                     YGKGGYCSGEITLKKETTLYLYVGLDGRKGYNFNGAGYGNGASGGGATDIRLIGGTWD
                     NEQGLLSRIIVAGGGGGTYDKQHGGDGGGLKGTLGTSSTGAAAHGGTQFEGGRGRDKD
                     GSCDGFFGKGATPENPSSQSGGGGGWFGGAYPASGFGNGSGGGSGYVLTKDSYKPPGY
                     TPTSEYYFDNVVMTTGGNTTVVGNYSDGRAKITLLQALPFLTVSSYNSTQATFKADHT
                     DPALLTKIEWFIDDVLKETITTDLTLEKTINYTLEDNALHTLKIVVTDSNNATAEKVL
                     SISKNIMPLPEENVNLNDISTKLVEVNAGFKVGKTSIINTLALKNIEASLNNTLVELS
                     EKIKTSFDSGDASVQDLMNQLTQANNTISQLNAKYKVAGGWTTPVYTNGTEKALVYNS
                     TREAIRYDWITISNLGFIPNIFYAECDYVNSYTKSKNKLFVFACYDVPTSSSKDFVFT
                     CDIQLGSSDSEYKVTYCGLYQHNKLDIHMTDNLIHLPAFATINAVASYYWRAIKIY"
     gene            64511..64804
                     /locus_tag="KPD58_000094"
     CDS             64511..64804
                     /locus_tag="KPD58_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0263890.1"
                     /translation="MNRANRIICDQTGKILLQTGEATGDILQHDTITELHCIDVEYGS
                     IDYTKNRIIGINIETKQPILEEIPVYLTEEEKRIQELENQILLNENEKVGGIL"
     gene            64804..64986
                     /locus_tag="KPD58_000095"
     CDS             64804..64986
                     /locus_tag="KPD58_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0263891.1"
                     /translation="MNINNVVVRILAERILSRGLNPLKNREFELDDVTNTDYRTAVED
                     YIIKQSGVVEGIEPTA"
     gene            65020..65586
                     /locus_tag="KPD58_000096"
     CDS             65020..65586
                     /locus_tag="KPD58_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0263892.1"
                     /translation="MTFKELVNKVRNLVLEAKNVTIEDTESKFTSENVEGALKECIDR
                     ADEAFQEADSGKTLLSTAIGSPATSEQTFQDYANYITGFKSNISNLETQLKSKYSIRH
                     GPIDGDDRNPFSANFGKSASYLIVYVYFRRNVYYYNPSGSSLGSSTGGSERAWITIDS
                     NKTGFSVHSYDTNYESYTFTGYYIACFA"
     gene            65617..65907
                     /locus_tag="KPD58_000097"
     CDS             65617..65907
                     /locus_tag="KPD58_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0263893.1"
                     /translation="MEEISINLLCAVAGVVISYLAFRNSSNRKIQDDTETTTKLEQQI
                     TFLCENVRDIKHDVAKFNTSFLDISERVAKVEASTKQAHLRIDEIINRIGGK"
     gene            65911..66168
                     /locus_tag="KPD58_000098"
     CDS             65911..66168
                     /locus_tag="KPD58_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBG0263894.1"
                     /translation="MDNLISFIPEQLLLLVVALNVLGFGFKKYKQLDNKYIPIILLVL
                     GIGFSIWMLGFNPSSILQGILCWGVAIGANQVYKQLKEENK"
     gene            66168..66983
                     /locus_tag="KPD58_000099"
     CDS             66168..66983
                     /locus_tag="KPD58_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG0263895.1"
                     /translation="MKIGINCGHTKTGPGSGAIGKINESIETRNVGYKVIDKLKKLGN
                     NVVDCTIDKASTQSECLSKITAQANRQDLDWFISIHFNAGGGKGCEVYTYKGKQYQDA
                     IDVCKKISDLGFTNRGVKDGSGLYVVKKTKAKSMLIEVCFVDSEDANKYLSLGADKLA
                     TAIVEAITKHISSAEENNYNRYKHTIVYSGDDKVSADILGLYYKREKESYLVTDIKDY
                     KPHRTQNLYVIGGVTCNKMKEMSKTTGEKFTQLYGNDVWSTMDKAIEFVKEKL"
     gene            67000..67296
                     /locus_tag="KPD58_000100"
     CDS             67000..67296
                     /locus_tag="KPD58_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0263896.1"
                     /translation="MLIQEKSFYPNDIYPKIDFLNIKRQLKSIYKNDLSDCGSICIIE
                     RKDYSMSVNSLGEVIIYYDSKFKQCVQDAIRDIELMFKSQIRSFYLIDRLEGSN"
BASE COUNT        27045 a         7295 c        12584 g        20550 t
ORIGIN      
        1 tttataatat caatgtattt ttctattaag atttcaaaaa agaataagtt taattattaa
       61 aattttgatt aatattatag cacaatagca caaaatataa ggagactaaa aatgaagaag
      121 tttataaaag tttcattttt tattttgggg atttttacac tattcatagc tataaataaa
      181 ttagtatata gagatatggt aagtgtcaaa gattatgaaa taaatcattt agaggaaatt
      241 gtttttgatt ttgatagcag gagaagtaga gatgttggta ctattgtaag atttaaaata
      301 agcaataata gtaaatatag ttataaatta aacagtgcta aaatgaagtt tgaaaattgt
      361 gtggaaaata aaaagggaat gaataagtgt tctgcatatg taaatgtaga tttatttaat
      421 gaaaaaaatt ctataactga aagaactctg aaatatggaa taaaaccaaa taatcaaggt
      481 tatgttgatt ttgtaattcc aaaaggacta aattttgatt ataggtactt taatgaatct
      541 ggtatgagtg tagagtataa aggagaatat attgtcaata ttccaatggc aaaaggactt
      601 tatctagtag ttggaaaaaa taatgataca tggaatgcta atattatgga ataatttgaa
      661 aaataaatca attatagata taattaagaa taatatgaaa tttataaaag ttcagactag
      721 gaggggtatt taatgtcaat aagtaatgta agagagtatt ttaaacaatt tggcaaggaa
      781 gacagtatat tagaatttga acaatctagt gctactgtgg aacttgcagc tgaagcagct
      841 ggggttattc cagcaagaat agctaagaca ctctcattta aaataggaga tgatgcaatt
      901 ttaattgtga cagctggtga tgctaagatt gataataaga agtataaggc tgaatttaat
      961 tgtaaggcta aaatgttgac accagaagaa gtcttggagt ttactggaca tgctattggt
     1021 ggagtctgtc catttggttt aaaaaattct ataaaagtct atttagatga ttcaatgaaa
     1081 agatttgata cagtatttcc tgcatgtggc agtagtaact ctgcaataga acttacatgt
     1141 gaagaaatgg agaagttttc taagtgtgaa aaatgggtag atgtttgtaa gaattggtaa
     1201 aaaaatatag taaactatag taggaattag taaaaaacta cctctgtata caatctatac
     1261 agaggtagtt tttttgtgta aatatttata aatctaagag gaaaatctaa tttaaactaa
     1321 taatccaaaa taaagttaaa aaattctgat tttttttcga aacaataaaa aaatgacaaa
     1381 aaagtggaaa aaatgtaata aatattgtta aaaaaataat tagaaattat tgaaaaatta
     1441 tcctttgagt gatactatta tagatgtaag atatttaaaa ataaaaaata gtcagataaa
     1501 aatacgatta tttagctcta ttaaaataat ttttatacga taaaaatctt ctgtacatgg
     1561 ctaaaaaaca gatgtacaga caatatttaa tgtttaatat accctaatat aaatatcgta
     1621 aacaaattat tttatctgta accagtgtga aagaaatctt attctaactt gaaatttcac
     1681 aaaattccaa aaagcgagat tttttttaag tatagttgat tttaaattaa caatttatga
     1741 ttaaattaat gtcataattg gttagtataa tcacatataa atatagagcg tagtttgatt
     1801 aaggttataa atttctcgac gtatattcaa ataccttatt atagatgttg ccgagatttt
     1861 aattaataaa tgaaaataaa atatttataa aatactgagg aggctattat ggatttaaat
     1921 tctaaaaaat atcagatgct taaagagcta tatgtaagct tcgctgaaaa tgaagttaaa
     1981 cctttagcaa cagaacttga tgaagaagaa agatttcctt atgaaacagt ggaaaaaatg
     2041 gcaaaagcag gaatgatggg tataccatat ccaaaagaat atggtggaga aggtggagac
     2101 actgtaggat atataatggc agttgaagaa ttgtctagag tttgtggtac tacaggagtt
     2161 atattatcag ctcatacatc tcttggctca tggcctatat atcaatatgg taatgaagaa
     2221 caaaaacaaa aattcttaag accactagca agtggagaaa aattaggagc atttggtctt
     2281 actgagccta atgctggtac agatgcgtct ggccaacaaa caactgctgt tttagacggg
     2341 gatgaataca tacttaatgg ctcaaaaata tttataacaa atgcaatagc tggtgacata
     2401 tatgtagtaa tggcaatgac tgataaatct aaggggaaca aaggaatatc agcatttata
     2461 gttgaaaaag gaactcctgg gtttagcttt ggagttaaag aaaagaaaat gggtataaga
     2521 ggttcagcta cgagtgaatt aatatttgag gattgcagaa tacctaaaga aaatttactt
     2581 ggaaaagaag gtcaaggatt taagatagca atgtctactc ttgatggtgg tagaattggt
     2641 atagctgcac aagctttagg tttagcacaa ggtgctcttg atgaaactgt taaatatgta
     2701 aaagaaagag tacaatttgg tagaccatta tcaaaattcc aaaatacaca attccaatta
     2761 gctgatatgg aagttaaggt acaagcggct agacaccttg tatatcaagc agctataaat
     2821 aaagacttag gaaaacctta tggagtagaa gcagcaatgg caaaattatt tgcagctgaa
     2881 acagctatgg aagttactac aaaagctgta caacttcatg gaggatatgg atacactcgt
     2941 gactatccag tagaaagaat gatgagagat gctaagataa ctgaaatata tgaaggaact
     3001 agtgaagttc aaagaatggt tatttcagga aaattattaa aatagataaa tattaaaata
     3061 tacacaatag gtcgatatta aaatagatat ggaggaagga tttatgaata tagtcgtttg
     3121 tataaaacaa gttccagata caacagaagt taaactagat cctaatacag gtactttaat
     3181 tagagatgga gtaccaagta taataaaccc tgatgataaa gcaggtttag aagaagctat
     3241 aaaattaaaa gaagaaatgg gtgctcatgt aactgttata acaatgggac ctcctcaagc
     3301 agatatggct ttaaaagaag ctttagcaat gggtgcagat agaggtatat tattaacaga
     3361 tagagcattt gcaggtgctg atacttgggc aacttcatca gcattagcag gagcattaaa
     3421 aaatatagat tttgatatta taatagctgg aagacaggcg atagatggag atactgcaca
     3481 agttggacct caaatagctg aacatttaaa tcttccatca ataacatatg ctgaagaaat
     3541 aaaaactgaa ggtgaatatg tactagtaaa aagacaattt gaagattgtt gccatgactt
     3601 aaaagttaaa atgccatgcc ttataacaac tcttaaagat atgaacacac caagatacat
     3661 gaaagttgga agaatatatg atgctttcga aaatgatgta gtagaaacat ggactgtaaa
     3721 agatatagaa gttgaccctt ctaatttagg tcttaaaggt tctccaacta gtgtatttaa
     3781 atcatttaca aaatcagtta aaccagctgg tacaatatac aatgaagatg cgaaaacatc
     3841 agctggaatt atcatagata aattaaaaga gaagtatatc atataattta aggggggata
     3901 aagaatgggt aacgttttag tagtaataga acaaagagaa aatgtaattc aaactgtttc
     3961 tttagaatta ctaggaaagg ctacagaaat agcaaaagat tatgatacaa aagtttctgc
     4021 attactttta ggtagtaagg tagaaggttt aatagataca ttagcacact atggtgcaga
     4081 tgaggtaata gtagtagatg atgaagcttt agcagtgtat acaactgaac catatacaaa
     4141 agcagcttat gaagcaataa aagcagctga ccctatagtt gtattatttg gtgcaacttc
     4201 aataggtaga gatttagcac ctagagtttc tgctagaata catacaggtc ttactgctga
     4261 ctgtacaggt cttgcagtag ctgaagatac aaaattatta ttaatgacaa gacctgcctt
     4321 tggtggaaat ataatggcaa caatagtttg taaagatttc agacctcaaa tgtctacagt
     4381 tagaccaggg gttatgaaga aaaatgaacc tgatgaaact aaagaagctg taattaaccg
     4441 tttcaaggta gaatttaatg atgctgataa attagttcaa gttgtacaag taataaaaga
     4501 agctaaaaaa caagttaaaa tagaagatgc taagatatta gtttctgctg gacgtggaat
     4561 gggtggaaaa gaaaacttag acatacttta tgaattagct gaaattatag gtggagaagt
     4621 ttctggttct cgtgccacta tagatgcagg ttggttagat aaagcaagac aagttggtca
     4681 aactggtaaa actgtaagac cagaccttta tatagcatgt ggtatatctg gagcaataca
     4741 acatatagct ggtatggaag atgctgagtt tatagttgct ataaataaaa atccagaagc
     4801 tccaatattt aaatatgctg atgttggtat agttggagat gttcataaag tgcttccaga
     4861 acttatcagt cagttaagtg ttgcaaaaga aaaaggtgaa gttttagcta actaatttct
     4921 tggggagggt attatgagta caagtgatgt taaagtttat gagaatgtag ctgttgaagt
     4981 agatggaaat atatgtacag tgaaaatgaa tagacctaaa gcccttaatg caataaattc
     5041 aaagacttta gaagaacttt atgaagtatt tgtagatatt aataatgatg aaactattga
     5101 tgttgtaata ttgacagggg aaggaaaggc atttgtagct ggagcagata ttgcatacat
     5161 gaaagattta gatgctgtag ctgctaaaga ttttagtatc ttaggagcaa aagcttttgg
     5221 agaaatagaa aatagtaaaa aagtagtgat agctgctgta aacggatttg ctttaggtgg
     5281 aggatgtgaa cttgcaatgg catgtgatat aagaattgca tctgctaaag ctaaatttgg
     5341 tcagccagaa gtaactcttg gaataactcc aggatatgga ggaactcaaa gacttacaag
     5401 attggttgga atggcaaaag caaaagaatt aatctttaca ggtcaagtta taaaagctga
     5461 tgaagctgaa aaaatagggc tagtaaatag agtcgttgag ccagacattt taatagaaga
     5521 agttgagaaa ttagctaaga taatagctaa aaatgctcag cttgcagtta gatactctaa
     5581 agaagcaata caacttggtg ctcaaactga tataaatact ggaatagata tagaatctaa
     5641 tttatttggt ctttgttttt caactaaaga ccaaaaagaa ggaatgtcag ctttcgttga
     5701 aaagagagaa gctaacttta taaaagggta aaaatctaat taaaaatata tttgctgtta
     5761 actagattaa atagtatttt aatctagtta acagaatata aatactaaaa taacaggagg
     5821 ttataactat gaaattagct gtaataggta gtggaactat gggaagtggt attgtacaaa
     5881 cttttgcaag ttgtggacat gatgtatgtt taaagagtag aactcaaggt gctatagata
     5941 aatgtttagc tttattagat aaaaatttaa ctaagttagt tactaaggga aaaatggatg
     6001 aagctacaaa agcagaaata ttaagtcatg ttagttcaac tactaactat gaagatttaa
     6061 aagatatgga tttaataata gaagcatctg tagaagacat gaatataaag aaagatgttt
     6121 tcaagttact agatgaatta tgtaaagaag atactatctt ggcaacaaat acttcatcat
     6181 tatctataac agaaatagct tcttctacta agagaccaga taaagttata ggaatgcatt
     6241 tctttaatcc agttcctatg atgaaattag ttgaagttat aagtggtcag ttaacatcaa
     6301 aagttacttt tgatacagta tttgaattat ctaagagtat caataaagta ccagtagatg
     6361 tatctgaatc tcctggattt gtagtaaata gaatacttat acctatgata aatgaagctg
     6421 ttggtatata tgcagatggt gttgcaagta aagaagaaat agatgaagct atgaaattag
     6481 gagcaaacca tccaatggga ccactagcat taggtgattt aatcggatta gatgttgttt
     6541 tagccataat gaacgtttta tatactgagt ttggagatac taaatataga cctcatccac
     6601 ttttagctaa aatggttaga gctaatcaat taggaagaaa aactaagata ggattctatg
     6661 attataataa ataagaattt gggaattaaa aatttaaata aaattgttta aaaaacaatt
     6721 tcggatatat gaaaaatcta atttaatggg ggtaatgaat atgagagaag tagtaattgc
     6781 cagtgcagct agaacagcag taggaagttt tggaggagca tttaaatcag tttcagcggt
     6841 agagttaggg gtaacagcag ctaaagaagc tataaaaaga gctaacataa ctccagatat
     6901 gatagatgaa tctcttttag ggggagtact tacagcaggt cttggacaaa atatagcaag
     6961 acaaatagca ttaggagcag gaataccagt agaaaaacca gctatgacta taaatatagt
     7021 ttgtggttct ggattaagat ctgtttcaat ggcatctcaa cttatagcat taggtgatgc
     7081 tgatataatg ttagttggtg gagctgaaaa catgagtatg tctccttatt tagtaccaag
     7141 tgcgagatat ggtgcaagaa tgggtgatgc tgcttttgtt gattcaatga taaaagatgg
     7201 attatcagac atatttaata actatcacat gggtattact gctgaaaaca tagcagagca
     7261 atggaatata actagagaag aacaagatga attagctctt gcaagtcaaa ataaagctga
     7321 aaaagctcaa gctgaaggaa aatttgatga agaaatagtt cctgttgtta taaaaggaag
     7381 aaaaggtgac actgtagtag ataaagatga atatattaag cctggcacta caatggagaa
     7441 acttgctaag ttaagacctg catttaaaaa agatggaaca gttactgctg gtaatgcatc
     7501 aggaataaat gatggtgctg ctatgctagt agtaatggct aaagaaaaag ctgaagaact
     7561 aggaatagag cctcttgcaa ctatagtttc ttatggaaca gctggtgttg accctaaaat
     7621 aatgggatat ggaccagttc cagcaactaa aaaagcttta gaagctgcta atatgactat
     7681 tgaagatata gatttagttg aagctaatga ggcatttgct gcccaatctg tagctgtaat
     7741 aagagactta aatatagata tgaacaaagt taatgttaat ggtggagcaa tagctatagg
     7801 acatccaata ggatgctcag gagcaagaat acttactaca cttttatatg aaatgaagag
     7861 aagagatgct aaaactggtc ttgctacact ttgtataggt ggaggaatgg gaactacttt
     7921 aatagttaag agatagtttt agattataat atttacaaat taaatattta attatgaatt
     7981 taacctgtaa gaataactat ttgttattct tacaggtttt tatagttaaa aataattatt
     8041 atctattaaa caaaaatagt gtaaaatata taatatcatg attttgaaga tataaaaagt
     8101 aggaagatat aaaagaaagt aggtaaaagt ttgtttaaaa aagagaatca aagatataat
     8161 cttatatcgt atacaaatga agaagaaatg actttaaaag aagttttatt ggacaagcta
     8221 aatttctcag ttaggtcatt atcaaaaatg aagagggaaa agagtgtttt agtaaatgga
     8281 gtatataaaa aaccgagttt aaaggtgtat agtggtgatt taatagaagt aaaaatagac
     8341 gaggaaaaag caaattttga acctcaagat ttaaatttac agataattta tgatgatttt
     8401 gatataatta tggtgaataa acctccgttt atggtagttc acccaacaaa aagccactat
     8461 gataaaacaa tagcaaatgg tattagttac tatatagata atcaaaaaga aaatgtaaaa
     8521 ataagatttg taaataggtt agatatgaat acttcagggt tagttatagt agcaaaaaat
     8581 gcttatgcac atcatacttt gtctacagca atgagtgaaa ataaagttga aaaaaagtat
     8641 ataacagtag ttgatggaat tataaaagaa aatgagggaa ctatagatga gccaatttat
     8701 agacctacag aagattcaat aaagaggata atagatgaaa gaggacagtc atctgttact
     8761 cactataaag taatagaaag actagaaaat gcaactgtat tagaagtaag cttagaaact
     8821 ggtagaacac atcaaataag agtacatatg gctcatatag gtcatggaat aataggtgat
     8881 gagttgtatg gttatgtaga tgaagagcta ataaataggc aagcacttca tgcatataag
     8941 ttagagtttg aacaaccaag aacaaaagaa aaattgaaat ttaaagcaga tataccagag
     9001 gatatgaaag agttaatatc gaaattaaga taatggggtg atatatatga taatacataa
     9061 atttataata catgttttgg acaaaaatag tgatgttcca atattaaatg actttgaagg
     9121 caaagttaat caagaagtag atggtttttt ccagaaagct ataaaaagaa ttgcaaaaga
     9181 tgaggattta agaaaaggtg tttttaaaga ttataatgac aatttaatta aaaactgttg
     9241 tgaacaaata atatatgatg aaagtacttt tttgaaaaat tctaaagaaa ttgcatctta
     9301 cttgtttgac gttatgaaaa ttaatgctgc actagaatct tgtgatttgg caatttgttt
     9361 atacactatt aaagatgaaa agagtgtagc aatactgaaa ttagattata aaaagttgta
     9421 cactcattca attgaatttg tggatgataa atttaatatt caaatggttt caaatgaaat
     9481 tggtatccca gagactttaa gacaaaagca aggagctttg attagtctaa gtggtataaa
     9541 tgatgagttt catctaaggc ttttagataa ggatgctgaa aaagaagggt cagaatctaa
     9601 gtttgtaaca gaatttttaa atgctaaaaa aatagatgat gataagtaca agaccaaggt
     9661 atttaaaaat acagctgaaa attggataac caatgctctt agtaatgata taaagcaagc
     9721 tgaagatgta agaagtatat taaattatac actgaaggaa aaacatgaag ttgatataaa
     9781 tgactttgtt gataattcaa ttaaagatga cgagttaaag gatagcttta aagagcatat
     9841 ggaagaaaaa ggtcttgatg aaagttttag tatagataaa aaatgggttg agaaaaagct
     9901 taaaaaaaga agtataaaaa cggataatgg ttttgacata aaagggaatc taactgattt
     9961 tgaagaccca atgaagtata ctgtaaaaca aaatcaagat ggaactatag atataataat
    10021 aaagaatgtt acgttttatg aagaaaagta atttatatac ttagtatata aattaaatat
    10081 aagagcgtgt attttatata ttgtaaataa atgtaatcta gtctgtgcga actctaatat
    10141 aatttattat gtagaagttt acacagacta tttatatttg tagacaaaac gatactattt
    10201 gttttcttcg atatatttac atatatcacc aatacattta aagttctcag cttcctcgtc
    10261 tggtatttct attccaaatt catcttcaag ggccattata atttcaacag catctaatga
    10321 atcagcttct aagtcgtcca ttaaagatgc ttccatagtt atctcatcta aattgtctac
    10381 ccctaattgt tctgctatta tttcttttat tttctcaaac attttaagcc tcctaaagca
    10441 gttttaatta attttaatgt tagtttaata taatatcaag aatttttcaa tatggaatct
    10501 ataaaaaata ataaaatttt ataaatgtag taataaagta ttatttgatg aataaacttt
    10561 attgataaca aatataactt aggaggaaat gctatgaaat atgtaggact attactaact
    10621 tcagtaggaa tgtttttact tatagcagta aatttttatt ataattcaat aactttagat
    10681 atgcaacgta tagaggatta tgtaatggag accaatttaa tccttgaaga tgtagctgaa
    10741 aaggagagtt atgtatctaa tgaaaaagaa gactacatat caaggttaat gcatgtaaag
    10801 aaaggaatag aaaattcaaa aacatccttc ttgatagaaa gatataagga gtacaaaata
    10861 aaatcaatag aaagtttaat ctatactata tcagaggaga aaaaagatta tctggatgaa
    10921 gttgatagat acaataaact tggtgaaaaa gaaataaata aactaatcaa taaaaacttt
    10981 ttagaagtaa cttatctgtc tattactaca tatatttaat taaagagaca aaacttagtc
    11041 tcttaatgat tcaaggagga ttagttatga caaaattaga agacaatatg gaacgtttct
    11101 ttggaggatg cgaaggattt tttgggaata aaatgttgat aataatagtt atagtattct
    11161 tactattatg cacagacatt ttagaagact tattatgcga tgacaatata tggatatggg
    11221 ttatattaat agtattgtta ttatttaact tcgatgatgg ttgttgttgc taaaaaatat
    11281 tgattattaa ataggtaaaa ccagtaactt atacaagtta tggttttacc ttttttagtt
    11341 caaattaaaa aatttaaaaa aatagtaaaa atacattagc aaaatggaaa ccaaatgcat
    11401 agtatatgtt aggagcgaaa agctcaacaa aatatcaaca ggaggtaacg tataatgtta
    11461 gatagattct ttggagaaga aggatgtttt ggtggaggag gatggtggat aatagtacta
    11521 ttcttcctat tcttagcttt tggagatagc tgggcagaat ttgacataat ggcatggata
    11581 ccattcctaa tagttttatt aatactttgc tgctgtggtg gatgcttaga ataagaacac
    11641 agttattaaa aaaaggaggg ctttgttatg ttagatagat tttttggaga agaaggatgc
    11701 tttggaggag gaggatggtg gataatagta ctattcttcc tattcttagc ttttgaagat
    11761 tgctggagag acattgacat aatgtcatgg atacctttct taatattatt gctaataact
    11821 tgttcatgtg gtggattctt tgatggtgaa gtaggttgcg gctgctaata cttaaatcat
    11881 tagaataaaa aaatgctggt attttatacc agcatttttt tattttattt tttaatatga
    11941 tttaaattaa gaatgaatat tattaaattt gttgaatata gggtataata taaaatataa
    12001 tgtatttaaa tgtacttgct tttatggtaa aataaaaact tatataaatg tagttaaaat
    12061 aggaaccttc aaaaatttgg ttcagtttta gataaattta gtaaaataag gaggatgaag
    12121 atgaaaggca atataacgat aaaagatgtt gctaaacaag caggagtgtc aatatctact
    12181 gtatctagag ttataaatga ttcaaaacct gtaactgatg aagtcaaaca aaaagtttta
    12241 gaggttataa aagagactgg atatatacca aatccacttg ctagaagctt agtaacaaag
    12301 aagagtcaat taataggggt aatagttcca gaagtttcag attcttttgt taatgaggtg
    12361 ttaaatggga tagaagaggt tgctaaaatg tatgactatg atattctttt agcgaataca
    12421 tactctgata aggaacaaga acttaagagt ataaatctat tgagagcaaa acaagtggaa
    12481 ggtatagtta tgatttcatg gatagttgaa caagaacata tcaactatat acaaaattgt
    12541 ggaataccag cgacatatat aagtaaaact gctagaaatt atgatatata tacagtaagt
    12601 actagcaacg aagaagctac ttttgatatg acagagcatc ttataaagaa aggtcatgaa
    12661 aagatagctt ttataatgac gagtaaagat gatactgttt tagaaatgga aagacttgct
    12721 ggttatgaga aagcactttc aaataacaat atagaattag acaagagttt gattaagtat
    12781 ggtggaactg attatgagag tggatacaat agtatgaaag aactattaga tgatggaata
    12841 atacctcatg cggcttttgt aacaggtgat gaggctgcca taggtgctat aaatgctata
    12901 tgtgatgctg gatataaggt tccagaagac atatctgttg caggatttaa tgatgttaag
    12961 atagctagaa tgtatagacc taaacttact acagtatatc aacctctata cgatatggga
    13021 gcagtagcaa taagaatggt tataaaatta ataaataagg aattaattga aaataagaaa
    13081 atagaattac cttatagaat tgttgataga gaaagtgtta cagaaagaaa aaaataaaac
    13141 tattaaaatc aatcaggtag tgcctaaggc actacctgat tttttatgtc aataatatca
    13201 aatttaataa tagattaact aaaaataatt atctaattac ttaaatagtt tatttttatc
    13261 tattcatgag ggttagactt tttagttttc ttaaataaac ttttagattt tttttgttga
    13321 ctacctggtg tattttttgg tgcaggtgaa tttgacttac ctttagtagg tttagccttc
    13381 ttagttttag ctggtttagt atctctttgg ttttctgcat ctgagatttt ttccattata
    13441 ttatccagtt ttttgcgttg attttcatct aaaaatgctt tgaattttga tactacttca
    13501 tctctgccat ctagattctt acctattttt ataagttcct ccataagttc attttctgat
    13561 tttcctttgt aactatcagc catcttttct attttatcct tgtcaatatt tttttgttta
    13621 gccatttttt ctaatgactc tgtatctaat tttttcataa aaaaacccct ttctataaat
    13681 atatattgaa ttatattcat actatttata tatagaaata atattgaagt ggtgaattgt
    13741 gagtgatata taatataaat aaaaattctt atagataata tatatgcact gagctttaaa
    13801 aatggaacat taaaatatga aaaacatact ttaatatagg gcaattatat aatataaggg
    13861 ggaattgtct tgaataactt actatttttg attggagtga ttgcattgag taatggtaat
    13921 atctctatat ttgatgataa aaataaatca aaaggaaata atagagttag aaaaaacaaa
    13981 aaaaatgcaa atgataaact ttcacagtct acacatggca atttaaggtt tgatgcaaat
    14041 gatataaaaa gaggtttaag gctcatggat ttaagtgaag aagatttaga aatgggaatg
    14101 gaaataataa caagaactaa aaaatacatg tcaagagatg aaagaaaaat actaataaaa
    14161 atagaaagtg ttttagattt agttagagga ataaaaaaat taaataatat agatatggta
    14221 gatacagaag atgaaacgga ttttttcaga aaaatggata gtgatgataa aaagaatatg
    14281 atgataaagg aaattataga tgtattccca gaaaaaagaa aaaattcagt tgaaaaagct
    14341 ataggaatga aaaagaagat agatttattt gcagaattat ttttaccaga tgactttggt
    14401 gaaggtggat ttagtttgag ctctttggca aatataaaca atcttggtag tatgaataat
    14461 ctcaaattgt taggaagtct tttaagaggt gatgatagta ataatgatga tgaagacaat
    14521 aatgatgagt taaattatga agacgaagaa ttagattgtg aagacaaaga attggatgat
    14581 gatgagttaa cagatgaagg tgaaatggag gataatgaag atgaattagt agaagataag
    14641 aacacaggct atgaaaatat agaaaatgat gaagatatag tgtacgatga ggaactatac
    14701 aacattaatg agcaagaatc cgaatacaat tataatgaaa aaaatactaa aactagagct
    14761 aagaaaaaac gataattaac taaatacata aagaaaaaag gaagaaaaag taatgataga
    14821 ataagattat ctcaattact atataaaacc actttaaaaa attcaaaatt tattatataa
    14881 taataatatg aaatcctgaa acatacgata aggctttttt aaattcaacc tacaaatgaa
    14941 atacgggaga ccgtgtctgg taaattataa catgcctagt tagtttggac gttaaaggtt
    15001 actggcaagt cacccacctg ggagtgccta gggtgtgaat ttacttagcc accggtgttt
    15061 cgggacaaaa aaagtgctac tatttcaaat agtagtactt tttttattaa tatgcatttt
    15121 tctactaaat acgcctaatt attgataaat tcaaaaagca agtaacaatt aagattataa
    15181 aagcatagta ttaattaggc ttcaaaaagc caaagactta ataagaatcg cttacaggga
    15241 gggcaatgaa aaatgcaaga ttataaaaaa aataaaagaa gaatgatgaa tcagccaatg
    15301 tctacaatga atgaagaaga agtgtataca gatgaaataa attcagaaga catgagaggt
    15361 tttaaaaaat cacaccatca taatggatgt aatactgata ataagtgtga gtgccatgat
    15421 gattgcaatc catgcaaccc atgtaatcca tgtaaaccta acccatgcaa tccatgcaaa
    15481 cctaatccat gtgatgacaa ttgtggatgc catgacaatt gtaaatgtga ttgtgaacca
    15541 tgtgaaatgg attcagatga atgttttgaa aacaaatgtg gaccagaatg ctgtaatcct
    15601 atatctccaa gaaacttctc tgtatcaaat gcagtgccat ttgcaataga ggctaataga
    15661 atatttgata ctatgcaatt ccaaacattt acagatgcaa caggaccaaa tggagagcca
    15721 ttaacttttg aaacagaagt agtagaagta tttggttcag ttccaagtgc aggtcaagca
    15781 agtgtaacta tagaaaaaat atgcttaagt aatgatggaa tcgttataga cacaggaatg
    15841 acaactttag aagatttcga tttagaccca ttaggagata tagtaggaag aaactgtgaa
    15901 acaacttttg aatttgcagt ttgtggagaa agaaactctg agtgctgtag acaaggaaaa
    15961 ggcaaatcag tagcttataa acaaagagga ttaactgtag cagttcgtaa tttagtacta
    16021 gagctaagag gtagatgtgg atgtacagag ttcgttgcat tagctttccc agcagttaga
    16081 gcaggaggtg gatgtaagag aagagttgat tatgtagaat ttacttttaa cacactttca
    16141 gcaccaatat gcttgccagc tgacggaaga gctgttactt taagacaaga atatcaaact
    16201 aacttaactg tagattgtat aggaaaatct atattaaaat tagaatgcaa cgaatgttgt
    16261 gaacctttct atgaattaat tataccaaat gatatagatt tagtactttg cttacaagaa
    16321 acagttagca cattaataag tgaacaaata gtagttttag catcaccaaa tccaatccaa
    16381 ccaagacttg ttgatacttt ctctaaagta tgtgattttt cgcaatgtgg acctaatcat
    16441 ggaagtggaa agccaagttg ccacagatag ataggtagat atatagacag tttaaattat
    16501 aaataaaaat catctaagga aggctaccaa agtgtataat ttacattggt agccttcacg
    16561 tttttataac tagcgtatag attttagtga tttaaaaagc actatagtat gtaatagaca
    16621 agtaaagatt gatactatcg tcattgtgta tatgtatgca taaatattaa gtcctggaat
    16681 tggaagaaat acatataaag ctattagttg aattaacatg ccagtaatag tatttatact
    16741 tgaagctaat tcttttctta ttgaatgtaa aatgccagat agagtttgat ttagggacat
    16801 aaatagtgga actaaaaaca tagcttttag atattctcct gcaagtgtat ttttaaatac
    16861 taatatacat aaatcttttc caaagaaata aaaaaatatt gatgatagta tgcctacaaa
    16921 taaagtaaga agtatggagt atttgatttt tttaattaca ctcttacgct ttcttagagc
    16981 catttcctgt gaaatagttg gtattaaatt tacaataagc gctgaaccaa cagtaaaagg
    17041 taaaaatata aaaggcatta ccatcccact gacaatacca tacatactta aagattgttg
    17101 ataagtcatt cctgataatg ctaatcttga tggtatcatc atagaactta tagattgcaa
    17161 aagtatgttt gacattctat tacaagttat gggaatagac atttttaatg tttccataga
    17221 agaattataa aaatctttta tgcttataat atacctattg tcaaatgatg aatctcgcca
    17281 taagcaaata agcataaata taatattact caactcacca atcgatatac ctaatagagc
    17341 tatataacaa ttcatagatt tattatttat gtacataaca agtaaaaaaa caaataatat
    17401 tttaccaatc tgttcaatga tttgtccaat agcagggatt tttacattct ttatgccata
    17461 gtaatatcct cttagtacgt ttgaaagagt tattataact atagcaggac aaacagctag
    17521 tataaataga tttaatttag catcttttaa aaatttaagt gataaaaaag aactattgaa
    17581 agatgcaact atagatataa ttagtgctac gaaaaatgat atatataaag ttgatataaa
    17641 taaagcatta gtattgtgtc tatcgtttaa agcttttctc tttgcaacta gacaactaag
    17701 tgcagtaggt atacctgttg ttgtaacagc taagcaaatc atcaaaaagt taaaaatcat
    17761 atgatagata cctaaacctg tttcacctag tgctcttgat aagaatattt tatataaaaa
    17821 accaaatatt cttacaataa aattggatat aaataaaata actgttgata gtactaaata
    17881 aggtactttc aaaaaaatca cctccttatc ttttaaatat attcacaagg aggtgtaaaa
    17941 atttataatt ttataaattc tggtttcaat ttattaaatt tagatacata tttaaatccc
    18001 atatttttta gtaatgaata tatgtagtca aactcacatg caacttgact tatatgatgt
    18061 gaatctgaac ctgtggttat gatttcccct cccaagtctt tatacatttg aagtatatac
    18121 ttagatgggg ttaggtctgg aagattatat ctataacaag aggtatttat ctcgatacct
    18181 ttgccatcat aaatagcttg tttaagagtt tcttcaatta tatcagaaaa tagtctatca
    18241 tctaaaacag tgtcataagg tgcatacctt ttaattaaat ctaaatgacc taagacagag
    18301 taatctttat aattcttaac aatattatat aaataaagat agtaattttc ataaacttca
    18361 tgttgagttt tatctttaaa ataattacca aggtataagt ccattgtatc tatagcatgg
    18421 atagaacata ttataaagtc caatggatat tgatgtgctt cctttttgta tgtatccatt
    18481 aattgagttt gaactccaaa ttcaattcct ttttttattg atattttatc tttatatttg
    18541 ctttgaagag tctctaaact cttaaaataa tcttcataga caattgaaaa agaatcatca
    18601 gcatatacat catagtcaac atggtctgtg aaacaaattt cctccaaacc gagttcaata
    18661 gattttttta ccatttgttc cattgtagat tttccgtctg tagagaaact tgagtgcatg
    18721 tgatagtcat aaaacataaa tatttcccct ttttataaaa attttggtat tatatataca
    18781 tactaaataa tatcaaatat acataaaagc atcaataaaa tataaataca atgagcggtg
    18841 ataaatttgg aaaaatacag aatagattta aatgagaaaa aatcaactaa atatatacag
    18901 atatttaatc atataaaaga attgataata aataaagagt tagtagaaca tgaaaaattg
    18961 cctccaataa gaaaactatc aaatttttta caagtaaata acactactat agttaaggta
    19021 tacgagcttt tagaaaatga aggatatgta tataaaatta tagggagtgg tacttttgta
    19081 tcaagtttaa attcttataa aagcggtata gataacagga aaaataagat tattgtgcaa
    19141 agagaagatt taattcattt tgacaatggg aatccatcta atgatatgtt tcctatcgat
    19201 tcatttaaaa atgctgtaaa tatggcatta tctaaagatg gtagttcaat atttgaatat
    19261 gatgaaggtc ttggctctga agaattaaga gaaaaaatgg tagagtatct aagtgatgga
    19321 aatataaaaa ctactaagga aaatattcaa ataatatcag gtgctcaaca aggtatagat
    19381 atagtttgta aaggacttat aagttattca gatgttgttt ttatggaaga accttcttac
    19441 aatggagcaa ttgaagtgtt taaaagtaga ggtgcaaaaa taatatctat acctatgtta
    19501 gatgatggaa ttgatatagg tattttaaaa ttaaagttgg aaaaaataaa accaagactt
    19561 atatacatta tgcctaattt tcaaaatcca actggaatat cttactcaac atataaaaaa
    19621 aagaaattga tagaattggc agaagaacat gatttttata taatagaaga tgattttata
    19681 agtgattttg tttttgaatc aaaggacaat agaacaatta gaagttatga tgataaaaat
    19741 agagtagtat atataaaaag cttttcaaaa atacttatgc caggtcttag gataggaatt
    19801 gtagaaatgc caaatgagtt gttaaaaaaa gttttatggg ctaaatattc atcagatata
    19861 tcaacaccag gtttaattca aaaatcaatg tattactata tgaataattt tgattggaag
    19921 aattatttga gtcatataga gaagatatat actgataagt ataaattggc aaaaaacttg
    19981 attaatgaca aattgagtgg taaattaaaa gttagaaaat cttgtggagg aataaacttt
    20041 tttttagaat taccaagagg atatacatct caagcattta caaattttat gttaaacaaa
    20101 ggagtgtcaa tgcttccagg gacttatttt tttgacaatc tagtggatga tagattcttt
    20161 aggataaata tagctcgtcc aagcatggaa gaactagaaa agggaatatc tataataagt
    20221 gataatatag atgagttttt ttgtgaatat aaaaacaagt tagatataaa aagcaataaa
    20281 ttattttatt aaataaatag aggtttttta ggaggaaaaa atgtttgaca agaagaaatt
    20341 agataggatt aatgaattag caaagaaaaa taaagaggga atcctttctg ctgatgaaat
    20401 caaagaaaga gaaattttaa ggaaagaata tttagaaaac tttagagccc actttagatc
    20461 aagactagat agtgtcaaag tagtttcacc agaagaatat gagcaataca tgaaaaacaa
    20521 taaaaattag aacttagtag gaggaaaata tgaacttaaa gtacgaattt aagaatgctt
    20581 gggattttga aagaaaagaa aatactattg aacaaattat gcaatattct cgcaattata
    20641 tggagttttt aagtaagtca aagactgaaa gactttcagt aaaagaaatt ataaaattgg
    20701 ctaaagaaaa taactatatt tcaatagatg aagctatgga aaaaggaagc ataagttgtg
    20761 gtgataaaat ttatgttata aataaagaga aggcagtagc actttttgta ataggtaaaa
    20821 attatataga aaaaggtatg aagattatag gaagccatat agattcgcca agattagatt
    20881 taaaaccaaa tccactgtat caagagagta atctaggttt ttttaagact cattattatg
    20941 gtggaataaa aaaataccaa tggacggcta ttccacttgc tttacatggt atagttatat
    21001 taaatgatgg aacaaaagtg gacatatcta ttggggaaga agatagtgac ccagtatttt
    21061 gtgtaactga tttattgatt catttggctg gagaccaaat gcaaaagaaa ctatcagaag
    21121 gaattagtgg agaagcttta aatatattaa ttgggaatat tcctttagaa gatgaagaaa
    21181 aagaacctat aacagcaaat atattaaaga ttttaaatga gaaatacaat atagttgaag
    21241 aagatttatt aagtgctgaa attgaagtag ttccagctgg taaggctaga gatttagggc
    21301 ttgataggtc tatggtgttg ggatatgggc atgatgatag ggtatgttca tatgctgctg
    21361 taaaagctat actagaaact gaacaaccag aatttacttc agttgcatta tgtgtagata
    21421 aagaagaaat tggttctaaa gggaatacag gtatgcactc aaagtttttt gaaaatactg
    21481 tggctgaact gattgcttta gaaggagatt attgtgatat taaagtaaga agagctttag
    21541 caaatagtaa ggtattatca gctgatgttt ctgctggata tgacccaaat tttggagaag
    21601 catatgaaaa gagaaattcg gcatatatgg gaaatggagt agttttaact aagtatacag
    21661 gttcaagagg aaagagtgga tgtaatgatg ctaatgctga atttatgtca gaagtaaaga
    21721 gaatttttaa taaaggaaat gtagtatggc aaactgctga acttggaaaa gtagaccaag
    21781 gaggaggcgg aactatagcc catatattgg caaatcaagg tgcagaggtt atagactgtg
    21841 gtgttggagt tttaaatatg catgcaccac atgaaattgt ctctaaagta gacatatatg
    21901 aaatgtataa gggatataag gcatttttta atataaattt ataagtaaaa atataaatgt
    21961 aataagtgaa taaaggctgt tgtataaaca atttaatctg ttgtacaaca gtcttatttt
    22021 tatgtaaaaa tagcaatttg tcacactaga gaaaataaaa gaaaatactg tcaaattaaa
    22081 aattatttaa aaaaaaattg acaacgattt catcttgtta taatatgtaa taaagaactt
    22141 gttatataat gttataaaaa tgttacatag ggaggtgagc aaaatggatg aaaagagaca
    22201 aaaaaaagaa atggatttag aagtaaattc ctttgttcct ttatatcaac aactatatga
    22261 caacataaaa aaacaaatag catctggcat atataaacca ggagataaac ttccatctga
    22321 aggagactta tgtaaagaat ttaatataag tcgtataact gtgagaaata cgactttttt
    22381 atataaaata gcaaaataac agtgtttgag caaaaaaaaa gaatgttttt tatcgttttg
    22441 ccacctgttt gccaccataa cttttttcgg tggcaaatta ttgtaaaatg ccctccaaaa
    22501 tatctattgt ttcactttcc attttacttg taacatgtga atatgtgtcc atagtggttg
    22561 ataattgact atgacctaat ctttgttgta tatattttac attagctcca ttttctaata
    22621 ataatgtggc atgtgtatgt cgcaagcaat gaaaattaaa gttaatattt agtttcttgt
    22681 taatagttct tatagcagcg tcaatattgt tgtgatttac aaatgagcca tctttttttc
    22741 tacaaaccca atcatactca gtttctttat accattttcc aattttaatt ttttgttttt
    22801 tttgattcaa tttttcctct tttaatatcc tagacaaagt atcacctatt ttaatatctc
    22861 ttattgatgt ttttgtctta ggtgatgcta attcaaattc tgaaactttt ctctttatta
    22921 aattttttct aactttgatt atattattat ctaaatcaac attatcccaa caaagaccta
    22981 atatttctcc ttttctcatg cctgtatgaa accctataag tagaggaata tagatatttg
    23041 tatttttagg atatatttct agtattttat tgaactcatc tagtgttata gtcttgttat
    23101 ctgactcatt ttttaccttt aaaacatttt ttggtatact ggcatattga acaggatttt
    23161 ccttaatgag tttgtaaggg taaacagcag attttaaagc agcatttaat acaacataaa
    23221 tcgcttttaa aactcctttt gtgtagtgtt tttcttctcc gttttgtgtg tatgtctctt
    23281 ttgatttatt atttaaaaat tcttgaataa tagctggatt tatagatttt agcttacatt
    23341 taccaagtct aggttctata tgattttcaa ttaaatttct ataactttcc tgagtattgt
    23401 atttgcaatt aagaagtaca tactctttgt accaaaagtt aagatagtct gataaactca
    23461 tatttgtttc atcaaacact ataccagcat tttcatattc atttattgca tcacggagcg
    23521 atttttcagc ttctttctta gtgtttccac caactctttc tacctttttt ctcttgccat
    23581 ctactatacc tgcatcaaag tagtaatacc acttctttcc acgttttctt acgccgcctt
    23641 tcataaaatc tctcctctca aaagcttttt ttatgtaatt atataataac atatatcaat
    23701 ttaaataaaa taaataaaaa agactataag aaacaaagtt ttatgtttat aattaatagt
    23761 cttttttatt tagatacata tttaatgttt tttatcacgt gattccagca ctttttctgc
    23821 caaataaaca actatttttt catactcaat atcagtttct aaagaaaagt caacaatagc
    23881 aggtatcgtc atatctcctg catatttatt acctaaccaa ttttcaatta attccaattc
    23941 ttctttgctt aattcaaatt ttttcttttt attaaaactt aagttcaatt tattcacctc
    24001 aaataatatt atatattaaa aatagtattt gatagaaaca ttcgaattat ttatgtaatt
    24061 ttcttttttt aatatattca tcagcaaaat aatttactaa atatttttcc tccaatccta
    24121 aagctatttc taattcaata agagcaggga tagttaaatc tttattctca ttattttcca
    24181 acctagaaat ttgacttctg tggcaaccaa ctctttttgc taactctatt tgtgttaatt
    24241 tcttcttttt tcgtaactct tttaacatat atttaaccta ccttttagat aattttatat
    24301 atgttagttt gtgtaatttc ttgtaataaa tgtgcaatat tcgcacactt tttgttggaa
    24361 atttgtgcta gaatgtagtt aagaaataac tttatctaga taaagcaaaa ataatagaag
    24421 ctataaaata taataatatt ttacagttat tttatagttt taagctttga aaaagaggtg
    24481 gttgtaacta tttttaagaa cgtatgtttc gtggaattaa taaataaaat attttatagg
    24541 ggatgatata tttgaataag acgaaatatt atgatatttt aaagttaaac ttattaatga
    24601 aaaagttgaa agaattggat aaaaataaaa tcaatgaata taaaataaaa gtgaaagaaa
    24661 tacataaaat caataaaaaa gaggaatgat tcctcttttt tattatattc attttcagaa
    24721 ataaactatt ttttattttt ttcgtctagt aaaaataatt ctgcaacttt taatgctttt
    24781 tctcttgcgt ctgggcttag ctcgctaaat atattaaaaa cttctttcat atcttcgtca
    24841 agatacatat tctcaataag ctccttttct gttttaaaat tttcagcatc ataatctttt
    24901 tcgtttttat ttataaataa gctatttcta acatcagttc ttcctaataa ataatctgta
    24961 gacacatcaa aatagtttgc atattcttct attgtacttt tcttaggttc tcttaaacca
    25021 ttttctatcc ttgatagtgt agatttgttt atatgtaaat cctcgcttaa tttgtctagc
    25081 gagatgcctt tttcttctct caattctttt atcctattca atttgcacaa ccccttttta
    25141 tattaattcc aaaatagcaa cttttattta tattatagca acaaaattaa ttattacaat
    25201 tttttttgct aaaaaagcaa caaaagtatt gactataatc acaaaacttg atattatata
    25261 aatatgaagt tgccaaaata gcaaaaagga agtgatttaa tgtacttaaa tagattagaa
    25321 ggattgatga aggaaaatag acacacccaa aaaaatgtgg cagatatatt aggtcttagc
    25381 tcatatggtt ttagattaaa attaaaagga aaaaacgaat ttaaagcaag tgaaataaaa
    25441 aagatatcta aattatataa tgtatctgcg gattattttt tttcagatga agttgctaaa
    25501 atagcaataa aagaagaaag gggcaataaa taatgaagaa tctaaccata atcaagcaaa
    25561 ataatcaatt tttagttgaa agtagagaag tagcagaatt aatagaaaag aagcacgata
    25621 atttattaag agatataaga ggatacaaga agattttaga ggactcatca aatttgaaga
    25681 gtcaagattt cttcatagaa agtacttata taaatactca aaataaaatt caaccttgct
    25741 acttattaac taaaaaaggt tgtgacatgg tagctaataa aatgacaggt gaaaagggaa
    25801 ttatatttac agcgatttat gtaactaagt ttgaagaaat ggagcgagag ttaaaagaac
    25861 aacaacctaa attaccaact acatataaag aagcactgca acagttatta atcgaagttg
    25921 aagaaaaaga gcaattacaa ttagaaaatc aagtaatgaa accaaaagca gattactttg
    25981 atgctctagt agaaagaaac ttgctaacta atataagaga tacagcaaaa gaacttggag
    26041 tcaaagaaaa aacatttgtt ttatggttaa tagagaagaa atattgttac agagatttaa
    26101 aaggcaaaat aaaaccttac tctaacaaga tgcagtattt tgaactgaaa gaatttacaa
    26161 caccatacgg acattcagat actcaaacat taataaatcc aaaaggaaga gaagcattta
    26221 gattattact tataaaggat ggactggtaa aagaaaaaga atatcaaata actttattag
    26281 gctaaaattg aaagtacttt gaaaattaaa gattatggag gtagttacaa tgagcgaaga
    26341 catattttat ggtataaaaa atactcttga tgaaatagca gaggttcaaa ttaaaaataa
    26401 acaaggtgtg ttaaaagaag ttggaatgtt aataagtggt gaggacaatt cttgtataac
    26461 gcattgttta gatgaagatt taattaagtt ttacataaaa gaagaggcag tattgacaat
    26521 agataaagat agtcatttgt tatgtatgct tgatgctcta ttttataact ttcttgataa
    26581 ataataaata cagaatattc ttgaaatggt ggtgaagaaa ttgggtaata tatctaactt
    26641 taatttagat aaacaagaag ataaaagttt taatgacctg gataatatat caatttgttt
    26701 ttcagaaggt attcgtaaag ttgtagaaat gaatttaaac aactataaaa ataaaatctc
    26761 aaaatactta aatgaaactt cgaaaataga attgttggaa ccaaaagaat taacaattgt
    26821 tataagtaaa ggttatcccg attatcttat gtctgttgaa gaagcaagta aaagattgaa
    26881 aatagataaa gtatttggat atgagttaat aaaaaatgga cttttgaagt cagttgatat
    26941 aggggcaact aaagtttcta gttatgaatt agatgatttt ataactagga atcaaggaaa
    27001 aaacatcaaa gaaatgctta gagaaatgaa agaacttaga gagggggtga tttaattgga
    27061 actagtaaca tacagaaaca agcttgtttt actaaaagat ggagaaaaga ttgcaactat
    27121 aagtttaaaa aggaaatttc tcagcaacag acttaaatta aaaataagat aggagagata
    27181 aattgaaaat aatttataaa aacaaagttt acaaagtaga acaagacaaa aagttattta
    27241 gaattacata ctatgatgag cagagaggta gtaagaagtt taataaagag aagaaaataa
    27301 aaagaagtgt tttaacaaga gatatagagt tagttaactt gtatttacca gcacatttaa
    27361 aaataaaata agctataaat aattaaagaa aaaggtgatt agatggaaat agaacaagca
    27421 acaatacgcc tgcctagaga acttaaagac aagcttctaa aacaggcaaa agttaaagga
    27481 tatacattaa aagatatgat agtttttatt ctaaaggatt atcttcaaaa tatttctcaa
    27541 gaataaattc aatttctcta cctatagaac gcttatcttt ttgagcaagt tgttcgattt
    27601 tctcaaaaag aagtttatta attcttagtg taaatctttt atcctcttca cgagtataaa
    27661 tatctttatt agacatattt atcattcctc acataaaatt tgacgtcttt atgacacttt
    27721 aataataaaa taatataaaa gaaaagtcaa caaaaatgct tgacgtcaaa tatatgacat
    27781 ggtattatta aaacaagaag ggagttgacg tcaaatatat gacaaatgaa agagttagat
    27841 ttacatttag attaccagca ccattgcttg aaaaaattaa aaatagagca tcaatagaag
    27901 gtagttcaat gaactcactt atattacaca tactttggga ttacataaaa gaaatagaaa
    27961 ataaggaggt caaataatgg ttgaattggt aaaagaattt gatttacaaa caattaaagt
    28021 aggaaatgca gtaaaagtaa attgcaaaag atttggtttt gaaattgatt gtatagtagt
    28081 agtagcaaca gaaaaagaat taaatttagc atactttgat gaaggtagag gctgtatgga
    28141 gtatcaagcc ttaataacag aagatattca agatggtgat tatgagatta aaattttatc
    28201 ttaggaggaa ataaaatggc tgctttaata atggtaggtt tatttgcaat atgtttagta
    28261 ggattagttc aaaatagaga ttaaattaag ggggattaat tatggaaagt ttaaaaagaa
    28321 taaaaaaaat ggttcaaaaa caattagttt tagctgaatt agagataaac aaaaatagca
    28381 aactttatga ggaacttgaa aataaggata gaggtttaat agatgatata cacatgagag
    28441 aatatctaag agaaaaagtt gcatgggaaa gagtaaaata tgctattgaa aatattttag
    28501 gtggtataaa tttagagatt aaatcaaagg aacatgaaga aagtgaggat tacaagatat
    28561 ttcaattaat tttagaagaa cttgaaagag ataaacctat agatgttcag atataagaaa
    28621 agagccattg ccgtggctca attcaaataa atatatcaaa atttaattaa gctaattata
    28681 gcacaaacgg agggaaatta tgagtacttt atatgaatta actacagatt tattagaaat
    28741 agaagaaggt ttaacagaaa caacaggaaa tgaaactgaa aaactagagg aaataaaaga
    28801 aataataaaa caagagatac aaaataaaaa cactaggata gtttcagtaa tattaaacat
    28861 tgacagtgat ataaactcta tagattcaga gattaaaaga ctgcaagagt taaaaagggt
    28921 caaaaagaat actcttgata gattaaaaag caatataaaa gactgtatgg aattacttgg
    28981 tactaaaaaa gtagaaacag ttttaggaaa tataagtata agaaagtcag caggtagctt
    29041 agtcatagaa gatgaagaaa agatacctgc tatatataaa acagtagagc aagttgtaaa
    29101 agtagataag aatatcatta aagactttat taaaaaaggt catgaggttg aaggttgcag
    29161 gattgaatat ggaactacac taacaattcc aaaagctaaa aaagagtagg tgaggaccat
    29221 ggaaactaat aatgtttata taaaacttgt aaatatacag agtactttaa aagctcctaa
    29281 aagtcaattt aatagctttg gtaaatacaa ctataggagt tgtgaggata tactagaagg
    29341 tttaaaacct attctaaagg aagaaaaagc attagttata ttggatgata atattgttca
    29401 gataggaaat agattttatg tagaagctac agcaacttta atagatgcag aaacaggaga
    29461 gaaagtatct acaaaggcat tagctagaga agatgaaact aaaaaaggta tggatttagc
    29521 acaagtaact ggaagtgtat caagttatgc aagaaagtac gctttaaatg gattattttg
    29581 tattgatgat acaaaagata gtgatgcaac aaataaacat ggaaatgagc agaaaaaaaa
    29641 agaagttaat gagagtgaat taaatacact atattcgcta ggagaatcta tagaaaaaga
    29701 taaaaataga gttgatagtg aagtatataa gaagtttgga aagttagcag tagatttgac
    29761 taagcaagag tatgagaaag ttttaaatgg atataagagc attttagaga agcaaaaaca
    29821 agagtaggtg atagtattgg ggattataag agtaagcaaa gacaaagata atccatatgt
    29881 agttttaaat aaaacttgtt tggaagatgt aaaattaagc tggcaagcaa aaggtttaca
    29941 ttcatatctg attagtaagc ccgaccactg gaaaatctat gttaatgacc tttgcaagag
    30001 aagtaaaaat ggaagagatg ctacagcaaa tattttaaaa gaacttatag aaaatggata
    30061 tataacaaga aaaccttgta gagattctaa tactaataaa atgcttggag ggtatgatta
    30121 tgaagtatat gagataccac ttgaaaatcc tcagaagcta aaatcccgaa aaactgattt
    30181 cccggaaacc ggatttcccg gaaaccgggt ttctcggaaa ccggaaaaca cggaagtagt
    30241 aagtaatgac tttaaagtaa ataatgatat tactactatt gttattaatg aacaatccaa
    30301 taaagacaaa accacctata taaaaaaata ctttgaaaaa tatataggtg tgattactcc
    30361 taataacttt atagagttaa tgagttactt agatgatgga atggaagctg atgtaataat
    30421 aagagctgtt gatgaagctg taggcagtgg agttaagaat tataagtatg taaagacaat
    30481 cttaaataat tggatagaag caggtgtaaa aactgtttta gaacttacag aatatcagaa
    30541 tgaatttgaa agaaagaaaa agagtaagca ggaaaagaag cagtctaata gtaaaactgt
    30601 gaatactcat aatgtgaata aaaataagtt tgctaacttc aatcagactt tcactcaata
    30661 tgaagaaaaa gagctagatg agattattaa aaagagtcag aaggaaaaat ttaaataaaa
    30721 ttaaacttct aggaagtaaa tatcaatata ttgcttccta gaaaagggga ggtataaaat
    30781 ggcgagaata tatgcacaaa gaagtggttc tttaaatgaa caagatagat tggagttatt
    30841 aagattactt gggaaagctg gatatacagt aaaggttgct agagagaagc aaaatagcaa
    30901 gacaacttat acttactttg ttgagtatac agaagaacag gaagaaaaat agaagggggc
    30961 tagttaaatg aatacaataa ctttagttgg aagattagtt gcagatgcag aattgaagta
    31021 ccttccaaat tcaggtactc caaaaataac cttttcaatg gcagtagata gaaggtttaa
    31081 agataaaaat ggaaataaaa taactgattt tattcaatgc gagcaattag gaaaacatgt
    31141 agagaattta gtgcaatatc ttgttaaagg taagcctata tatgctgttg gagagttaaa
    31201 tatatataat tacaaagatg aaaatggttg ctggaaatct attactaagg ttaatgtaaa
    31261 tgctttagaa ctactttcta gtaaaaatga taataatgct aaacaagaat atgtaccacc
    31321 aggattagac ccacaaggtt ttcaagcaat agatgatgac gatatacctt tttaattaag
    31381 ttaaataatc tagggagtaa ttatgcaata ttacttccta ggagttaaaa aatattggag
    31441 gtttaagagt gaaatatgag tgtgagaaag tgttcttaga atgcgataag ggaagttttg
    31501 agataaatga tacaagagct gaagaagtaa cattcgaggg tacagaaata gacaatctgt
    31561 ttgagagagt aaaatatgaa gggactttta caataatatc tggatgggat tctttattga
    31621 gagatatatt gtggcttgaa atgcggaaag tcttaaaaat tataacagga aaaatgatga
    31681 tgggggtatt aggaatgata ataattagaa gtcaagataa aactgattta gtaaaagctg
    31741 attggataaa tgttgataaa gaatgtgtat atgcaatgtt tggagatatg gataacttta
    31801 aaaagatagg tgagtatgag gatgaaaaaa gggctataca agtgctggat aatatacaaa
    31861 gatttattga aggtggaact agaacagatt ctatagacag ttataaggtt agaagttata
    31921 gaaatagaat atttcaaatg ccagttaaat agggaggtta ctgaaatgtt aaaggttgaa
    31981 aaatatttta gtggttcttt aggaacaaat atcatagatg atgaccctac atgtagaaat
    32041 tacttagcac tatattgttg tgttcacgga gtaagaaaaa atggagaact tatatttcca
    32101 acatgtgaaa aaatgttaat agagtttaat gttgataaaa atagaaaaaa gaaaaagaaa
    32161 ggttcaaagg taaagttgat taatgccaaa actggtgaag aaaaaatatt tgattctata
    32221 gatagtgcag catgtttttt aagactacag agtcaggcag tttaccaaac aattaaaaag
    32281 aaaactaaaa caagaagtgg ctggaaagct gaatatattg aggaggaata atggaagttt
    32341 caaggactga atacacaatt aaaagagcaa aagagttata tgataatgga gaggatatat
    32401 ttattgctat agataaggct agagaagaat atgaggagat ggttaaaagt gaatatctta
    32461 gctagtgtga tattagtaat aggaagtttt atagctggta gagtttatga gtatagattg
    32521 aatttaaatg aaaatgatga agctgattca aaagtacttt tagatgtttt taatgaaatt
    32581 agtgtgttaa gagaagaaaa taaaagcctt aaagaaaagt tacaagagaa agagttacta
    32641 ttcatcaata gattaataga ttttttacat gataaaaaga tatgcgaatg ttgtatttat
    32701 gactgtaaga ttgatgatat tgaatatgat tgtgaagatg gtattaaaaa gtggcttgat
    32761 agcgaagaac ttatatttga atagaagaaa tatctaatta aaacagttta gagagttgca
    32821 aaatgttttt taataaaatt atcattgaga tgttttgtaa ctctcaaaaa tgaaaataag
    32881 gaggcgttgt attgcttaca tttttagatt tattcgcagg gataggtggc tttaggctag
    32941 ggatggaaaa agcaggacat aaatgtttgg gacattgcga atatgataaa ttcgcaaatt
    33001 taagttataa tgccatgcac aaaccgaagg aggatgaatg gtttgaaaga gatattagag
    33061 aaattagaac agaaaatatc ccaagagcag atgtctggtg ttttggattc ccatgtcaag
    33121 acatttctgt tgcagggaaa caatttggat tcagaggaga acgttcaagt ttatttttta
    33181 cagttacaaa acttattaga gaactcaaag aagaagatag acccaagtat ttacttattg
    33241 aaaacgttaa aaatctactt agtgttaatg gaggatttga tttcctcaaa gttctcgttg
    33301 aactggatga aatcggctat gatgcagagt ggcaagttct taattctaaa aacttcggag
    33361 taccccaaaa tagagaacga atattcattg ttggacattt tagaggacga agtacacgaa
    33421 aagtatttcc tatcgaaaga aaaagtagaa aaaatcttga gcaactaaat aatccaactc
    33481 atagtacaaa tagaatttat gatgcagttg gaattgctag atgtattaga agtcaggcag
    33541 gaggtggagg tgctaaaaca ggtctatact ttatagactt aaataaaaac tctaaagtaa
    33601 caataaatgc tagatgcctt aaagcaaaat ataatgcagg tgtgacaaat agaaattgtg
    33661 ataatagtgg agttttagtt aatgcagttt taacgcccga tagggtaaat aaaagacaaa
    33721 atggtcgtag aattaaagaa agcggagaac caatgttcac attgacagct caagataaac
    33781 atggaatttt gaaaaatgga gatataagaa ggttaacacc aaaggaatgc tttaggttgc
    33841 aaggatttcc ggataaatat tacgaaagag cagcaagtgt atgctcagat agtcaattgt
    33901 acaagcaagc aggaaatgct gttactgcaa atgttgtata tgaaatagca aaaagaatgg
    33961 gctaaaagtt gcaaaaagtt gcaaaatgtc ttttaatatg aatatttttg aagtgttttg
    34021 taactctcaa aaatgaaaat aaggggtggg ataaatgtat gaatatatat taagatggca
    34081 aataggatta tcgttagaaa atagaaaaat acattataca tatggaagta aagaagcttt
    34141 aagaaagaaa gcaaaggcat tggctaaaga tgaaaatata gtactaataa ctatagataa
    34201 ggtagatgaa gttataaaaa atactataag cgagaagatt atagaacgct ttaaaaattt
    34261 ataaggggtg aaattatgat aatacacaaa tttataatac atgttttaga taagaatagc
    34321 gatacaccaa tactaaatga ctttgagggt agagtcagtc aagatattga agctttcttt
    34381 caaaagaaga taagcaaagt atcaagagat aatgacatca gaacagcagt atttaatgac
    34441 tatagtaaca atctaattaa gaagtgctgt gaacaaatta tttatgatga aagttcattt
    34501 ttaaataact ctaaagagat tgcagcttat ttatttgatg ttatgaagct taatgctaca
    34561 ttagaatctt gcgacttagc aatttgttta tactctcaaa aagatgaaaa gaaagttgct
    34621 atattaaagc ttgattataa caagtcatat actcattcaa tcgagtttaa agatgataaa
    34681 tttaacatac aaatgtctaa aaatgaaatt aacatacaag agactaagac agtcaaaatt
    34741 gctgctttgg ttggattgag tggaatgaat gacgaatacc atttaagagt attagacaag
    34801 gatgcagaaa aggaagaagc taattctaag tttgttacag agtttctaaa tgctactaaa
    34861 gtgaaagatg acaagtacaa gactaagatg tttaaagctt ttgtggactc ttatatagca
    34921 catttatata gtgatatgaa gcagggcgaa gatgtaagag gaatgttact ttatatgtta
    34981 agagaaaaac aaaaacttga tataaatgag tttgctgata aggcgataaa ggatgattta
    35041 aaagatagtt ttaaggacca tatagaagaa aaagggattg aaagttttaa tattgataaa
    35101 aaatgggttg aaaagaattt aaaaaataga catataaaaa cggatacagg ttttgagata
    35161 aaaggtaaaa tggaggattt tgaggatttt atgaaatatg gtattagaca taatggaaat
    35221 ggaactgtag atatagttat taaaaatgtt aatttatata atgagaagta ggtgctcata
    35281 tgaaatatgt agctagtttt agtggaggaa aagattcagc agcaatgctc cttttaatat
    35341 tagaaaaaag gcttctgtta gacgagattg tttttataga tacaggacta gaatttaaag
    35401 aaatttatga tataatagat gattttgaga aaagaataaa ctttaaaata acaagaatta
    35461 aagcagaaaa aacctttgaa gaatactttt atactgttaa taaacaaggt aagcgtaaag
    35521 ggcaaatatg gggttttcca tacaccttgg gagcatggtg taatagcaga ttaaaacttg
    35581 ctcctgctaa taaatatttc aattcgattg gagaacacaa gcgatatatc ggaatcgctt
    35641 ttgatgaacc tagcagatac agaaggctag aaaaaaactg catagcaccg ctttacgaag
    35701 caaaaatgac tgaaaaagat tgcttgaagt atttagaaaa aaaaggattt tactatgata
    35761 tacatcacag attcaagaga actggttgct atttgtgtcc taagcaaagt ttagatagcc
    35821 tcagaacttt aaggaaatac tatcctgatt tgtgggcggg tatgcttaaa ttggataaaa
    35881 atagtccaac gacttttaga gcagacggaa ctacagtaca tgacttagaa aagcgattta
    35941 gaaatgaaga tatagaaaat gaaagacaga taagtttctt tcaaacagag aaggtgagca
    36001 tatgactaat aaagaaatgt gcaagtcaaa taatcttgat gaaagagaag tgtgtaagag
    36061 ttttgggaaa gagatttgtg ctagttgtat aaatgataaa ggagattgtg aaagtaaaga
    36121 ttgtgatata gcatatgaga actggctgga gaaggagatt gtaaattatg tttagcatct
    36181 ataaagtgaa actaaagact aaaagaacat tggaacaggt aagaaatcaa agtgcagact
    36241 ttgagtattc agaagaagga ttaaaaaata cacttagata ttacaacttg attgatggtt
    36301 tagaagtaat tgtagttaag tttaaagatg aatattccct agctggttgt gatgaaaaag
    36361 atgtagaaaa aataagagat gcatattata ttttagaaca agatgaatat tttgggtgtt
    36421 atataaatga atatgaacga tttaaaaaag attgggaaaa tggtgaatgt ggtggggaaa
    36481 ttggtatgat gttttcagat gatgaagttg agataattga gaaattaagg gagggttaaa
    36541 tatgaatttt aaattaatta gtgatatacc agattggaca atagaggaac agttagcgaa
    36601 gattgatgaa gaagttacag aacttaaaga agcaatcttg gaaggtgaca aagagcatat
    36661 catggaggaa atcttagatg tttgccagtc tgcattaaca gcaactcaag tattaaaaat
    36721 agatgataaa ttatatgaag gaataaataa acacaataga aaacttggat acagaggatt
    36781 gtcacttcac aatataaaat taccatacag aataggaggt agaatatata taataactga
    36841 agaaaatatc ttttatgaag ctattcttct aggtgtagag attgataaac atgatttgtc
    36901 tttaaaaaac tatatagtct tagcacaaac atatgataga gagtttgtag ggtgttttaa
    36961 ggaaatttac actagcgaag aattagaagc agtttatgaa ggcagataaa taaatagtca
    37021 aggtaagttt atgaatgaaa ctagaatgtt atagacttac tttgacttat aaaaggagtg
    37081 tgttaaatgg ctaatatata ttgtgaaaat tataattgta aaaactactt tgaagatatg
    37141 tgtatgcttg aaagaattga aattaataac ttcaaagaat gcgaaagcta tcttgaaggt
    37201 aaaaatgagc tatataaatt agaaaacgga tatactatac atcctaaaga tttgaaaatg
    37261 gtgaaaagta aagattattc tgttgaagtt actcatattc caactggtat tacagtaaaa
    37321 tgccgttcta caaatagtat tttaaaaaat aaaaataagt gtttggaagt tctagaagaa
    37381 gaactaacaa aaataaactc tcacttagag ctagaagatt tacgctaaat aggaagtgag
    37441 cttatgaaac gaagaagatg cagttggtgt ggtaagttat tttatcttga agaaaaatct
    37501 aaggatgttt attgttgtaa agaatgtagg aagaaggcta agaaggtgaa aaaatgaaag
    37561 tttttcttgt aatagatgga gaaccagttg gtaaagaaag acctagattt aacttggcta
    37621 ctaaaaggac ctatacacca cagaaaacta aggactatga agaattaata aaatggttat
    37681 atcaatctaa agttagacat tattttactg gatacataaa aatgacttta agatgttatt
    37741 actctatagc aaaaagtaac agtaaaaaga ttaaagagca gaaaagaaat aatgtgttaa
    37801 gacccaataa aaaaccagat attgataatg tggtcaagat tatagctgat tcactcaatg
    37861 agatagctta taaagatgat acacagattg ttgaggttgt agctagtaaa tattacagtg
    37921 ataaaccaag agttgaggtt atattagaag atattagttg aacaacggaa aaattcgttt
    37981 atcaaatcat tagataaaaa taatttggag gaggattata agtatgaata atttaaaatt
    38041 aatcgagaat gaaggactta taaaagtata tacaacagat gaagatataa aggttgtaga
    38101 tggtagggaa ctttgggaag ggttagaagt agaaaaagac ttttctgact ggattaaaag
    38161 taacttagaa aatgtagatg cagcggaagg aatagacttt tccactttaa aggggaaaac
    38221 ctctgaacaa ggcggaagac ctgcaataga atatatatta aatttagaaa tagcaaaaga
    38281 aatttgttta gtagcaggag caagtccaag agcaaataaa gaacttaaaa gaaattctaa
    38341 aaattataga aaatatctaa ttgcagtaga agaaaaatac aaagtatcaa acaatcttac
    38401 aaaaacacag ttaaatcaaa ttaatgatat tgtaagtaac gcattatgtg aaatgcaaac
    38461 taagcatgat gcacagctag aacaatttaa aaaagaatcc tcacaatatt atagacctac
    38521 aagcaaaact aagtatgata tttcaagcta cataaaagat agattagata ttcctagagc
    38581 taatgaggag tttgatttag ttaaaaagag aactttatta attcttggag ctgataagtg
    38641 ggaagacata ccaaaagatg tgttattaaa atccctaaat attatagatg aatcaataag
    38701 aattataaaa tctgaaagaa agacaaaaca gattagtttc tttgaaaaag ataatttctg
    38761 ttaataaaaa gaaaaaaggg gtgctttcac actccacttg tcaaaaatat aaaactttta
    38821 tatacaaata ttattataac ataaaaaatt gatgggagtg tggaagtatg aataaaaaga
    38881 cactatttca agaggttgaa ggtagattat ataactataa aaaactagaa agtcaaataa
    38941 gaataaaaga tatatatatt aaaaaattag aaaacgaatt ttgtggatgc aaagctcaaa
    39001 gttatgaaga aaaaactgga gaaacttata atataaattc aagtgttgaa aatgaagtta
    39061 ttaaaagaga agaagattta aatagattaa aagaagataa gaaaacatta gagattgaaa
    39121 aagaaactat agaatgtgca ttgacaagtc taaatagctt tgaaacagag ttcttcaatg
    39181 aaatgtatat gaataatgaa aaaatcaaca tggattatat gtctaatgct atgcacatag
    39241 atagaagtca ttgctttaga ataagaaaaa ggatagtttg taagattatg gatatgttat
    39301 atccaaaaat aaaagagttt gaattaccca ttttttcatg gaaagcttaa aaatgagact
    39361 attttgagac tttttggaga ctatcgtgag actttttatt ggtaaaaaca tgagataata
    39421 atatcgtgga aataaagatt tccctctcaa aactaaatat atattaggct agggttaagg
    39481 gattactcta gcttatatga acagactagg cagggcatga ggatgctgtt agttcaattc
    39541 taactatgtt caatccttaa gtttttttct atttaaatta atctacggat acactaaaaa
    39601 tagtatatga attgagacta aaattcatac agttttgtat cttaattcag gtgtctaaat
    39661 cgagtggggc ttggtaacct cactcaccat gcaagggctg gtgtttaatc taagttcgat
    39721 tcttagaact tgcaacataa tatatgtacc tccctattat aaaaggctaa gcaagggcaa
    39781 gcttagtctt ttatttttaa aaaaggagaa taaaaatatg gatgatttaa gaggaaagat
    39841 aataaaagaa ttagatgaat ttaatataga tgcagatgat gaatttttag attatggagt
    39901 tgaatatgtt gagagtttta cagggaaaag tgctattagt gaagatttat tgataagggg
    39961 agtagtgctt atggcaaatt atatagtaaa tatggaaaat aaagacttag attaatttct
    40021 aggtcttttt taatacaaaa atttaataaa ttttagatgt cggtaactcc gacagtagaa
    40081 aagactcaga ttaatttcta ggtctttttt tattcccaaa acgacaaaca aacgaggtgg
    40141 tgatgtgcaa gatgtcaaag aaaaggtaaa acaagattac ctaaaaggta tgaaacaaaa
    40201 ggaaatatca gcaaagtatg acattagttt aaacacttta aagtcatgga ttaaaagata
    40261 caactgggct agtgaaaaaa agaagggtgc acctaaaaat aaaaggggtg cacccatagg
    40321 taataaaaat gctactggtc cacctggtaa taagaatgct gaaaagtttg gtttcttctc
    40381 aaaatatcta cctgaagaaa ctagggaatt gatacaagaa atatctataa aagataaatt
    40441 tgatattctt tgggaacaga taacaattca atatgcagca ataataagag cacagaagat
    40501 aatgtatgtt aaagacaaag aagaaatgat taaggaatta aagaaacatg aaagcacaga
    40561 aaatggtgag aagatagagt atgaatttca atttgcatgg gataggcaag catcttttct
    40621 taatgcacag agtagagcta tgagtgaact tagaagttta attaaacagt atgatgaaat
    40681 gattcataag gattggaatt tggctacaga ggagcaaaaa actagagttg agaagttaaa
    40741 atgtgaggtt gataacctaa gtaaggatga tattggagat gatgagttga aaataagtgt
    40801 agattatggt gatagaaatg atagttagaa taaaatttaa tccatctttt aaagaagcta
    40861 acttcactaa aaaaagatac agagcaatga aaggttcagc aggtagtgga aaatctgtta
    40921 atgtggcaca ggattatata ctaaagttag gagataaaaa gtatcaagga gctaacttat
    40981 tagtagttag aaaatcagaa gctacacata agtattcaac gtatgcagag cttacaggag
    41041 ctataaatcg tatttatggt aaacaagctg ataagtattg gaaaactact ttaaatcctt
    41101 tagaaattaa gagtaaagtt actggtaact ctataatttt cagaggagtt aatgatgcaa
    41161 aacaaagaga aaaattaaaa tcaattaact tctcgaaagg aaaattaaca tgggtttggt
    41221 gtgaagaagc tacagaactt atggaaagtg acatagacat actagacgac cgtttaagag
    41281 gtattttaac taatcctaac ttatactatc aaatgacatt tacatttaac ccagtatcag
    41341 ctactcattg gataaaaaga aagtattttg actataaaaa tgatgatata tttactcatc
    41401 atagtactta tctacaaaat agatttattg atgaagcgta ttatagaaga atgaaaatga
    41461 gagaggaaca agacccagaa gggtataaag tctatggact tggagaatgg ggagaaactg
    41521 gtggagcaat acttaaaaac tatgttatac atgaatttcc tacagaattt gaatactttg
    41581 ataatatgag gttatcacaa gactttggtt ttaatcatgc aaatgcaata cttagaattg
    41641 gctttaagga tggagagtta tatatatgta atgaaatata tgtacatgaa atggatacct
    41701 cagaaataat aacgattgca aatagtaaag gtttagaaaa gagtctattt atgtactgtg
    41761 atagcgctga acccgataga attaagatgt ggaaaagtgc aggatataaa gctaaaggag
    41821 ttaaaaaagg accaggaagt attaaagctc aaatagatta tttaaaacag ttaagaatac
    41881 atgtacatcc tagttgtata aatacaataa aagaaataca acaatggaaa tggaaacaag
    41941 acgaaagaac tggattatat cttgatgaac cagttgagtt tatggatgat gcaatggctg
    42001 ctcttagata ttctatagat aataaactta aaaataatgg ggttagaata cttactccaa
    42061 atggaagaag gtgagaaatt ggaactagat gtaataaaaa agttaattga acaaactaat
    42121 agtaaacata ataactttgt taaaaaggct gatgaagctg aaaaatacta taaaaatcaa
    42181 aatgacatta taagagatag aagccctaat aatgttggca aagtaaatac agccaataat
    42241 ccactaagaa atgcagacaa tagaatacca tttaattggt ttggtttttt agttaaccaa
    42301 aaagtatcat atctgtttac ttatccgcct acttttgatg ttggagatga tggtataaat
    42361 tcaaagataa ctgatatttt aggtgataga tacccaaaag aagccaaaac acttgggaaa
    42421 aatgctagta tatgctctaa agcatggtta catgtttggg aggatgataa caatgacttc
    42481 caatatgcta atatagaccc tcgccaaata agagcagtat attcatcaga tttaaacaga
    42541 aagcttcttg cagtacttag agaatataaa aagactgatg atgaaggaaa agaatatgta
    42601 atctatgagt attggacaga tgaatgttgt tatacttatc aaaataaaga tggaaatagt
    42661 aatatcaatg gattggagat acttaataaa tttatagaga aaaatttaga taataaactt
    42721 gaaactcaaa ctaatgcata taaacacaat tttggagaag ttccatttat cgaatttcta
    42781 aacaatgatt tagaggtaag agatttagat aatgttaaac acctcattga tgtatacgac
    42841 aaggtttata gcggttttgt gaatgatatt gaagatatac aagaagttat tttcgttctt
    42901 acgaactatg gaggtgcaga cttaacagag tttttaaaag gacttaaaga atataaaact
    42961 attgatttac aaagtagtgg tgcagatgat aagagtgggc ttagtacaat tacaataaat
    43021 attccaatag aagctcgaga ttcgcttctt aaaacaacag aaaagcaaat atatgtccaa
    43081 ggacaaggtg ttgaccctaa accagagaat ttcgctaata caagtggtgt agcactcaaa
    43141 tttttataca ctttattaga attaaaagca ggtttgatgg aaacagagtt tagactagga
    43201 tttgctaaac tagtaagaat aatatgtaag catataggat attcgcctaa aagagtttta
    43261 caaacttgga ctaggaatat gattcaaaat gatttagaac tggctgatat atgctctaag
    43321 agtgtaggga tagtatcaga aaagactaat ttaaaaaatc atcctcttgt agataatgca
    43381 gaagaagaag aaaaacaaat taaaaaagaa aaggaataca atcaacaaga atacaatagt
    43441 atcattccta ataatagtgg tgttataaat gaaacataaa gactattgga ggaaaagatt
    43501 tgaacaatta gaagaagctc aaaataacaa aagtataaaa tgctatcttg aattagaaaa
    43561 gcaatataaa ttagctatga ctagtataga aaaagatata ttagcatggt acaacagatt
    43621 tgctgaaagt gaaggaatat ctttattaga agctaagaaa ctactaaata caagagaact
    43681 agaagagttt aagtggaatg tagaagaata tattaaatat ggtaaagaaa atgctataaa
    43741 tcaaaagtgg ataaaagaat tagaaaatgc tagtgctaga gttcatatta caaggcttga
    43801 agctttaaag ttacaaatac agcaacaagt agaagtttta tatggaaatg aaattgatgg
    43861 tattgataaa ctaatgagag atatttatac aagcgagtac tatcatacag cttttaatgt
    43921 tcaacaagga gtaaacgttg gttggagttt aatgagtctt gatactaata gaataaataa
    43981 aattatttct aagccatgga ctagtgatgg attaaatttt agtgaaagga tttggagcaa
    44041 acatagacct gctttaatta atgagttaca cactaaattg acgcaatcaa ttattagagg
    44101 tgagaatcca aaaaatctag tcaatgactt tgctaagaag tttaatgtat ctaaatcaca
    44161 agctaagaat ttaataatga ctgaatcagc tttctttgca tcagcaagta gaaaagattg
    44221 ttttaatgat ttagatgtag agaaatatga gattattgct acattagatt taagaacttc
    44281 aaatatatgc agagaattag atggtaaagt atttgatatg aaagattatc aagttggaat
    44341 aacagctcca ccatttcatt gtcgttgtag gacaacgaca gctccttttt ttagtgatga
    44401 agaaggctat agagtagcaa gagatgaaaa tgggaaaaca tattatgtac catctaatat
    44461 gaaatataaa gagtggcata agaagtatgt taaaaacact tactaagtta gtaggtgtct
    44521 ttattatgta aaaaaattat tgagagggtg atttgaaatg cttaaattat atattttatc
    44581 aataattgtg ttttgtacag ggctttattt attcaacatg aaggtttata gtaatgagga
    44641 attaattgaa ttacttaaaa gtgtgaatat cagaaaaaga aaaaaatata attttatttt
    44701 tctagcttta tttccactac ttaattttat tcttggtgtg atactcatac tatattcttt
    44761 gctagttagc aatgaagata ttattaaata tttaaggagg aataaataag atggctaagt
    44821 ttaaaaagaa agcagttgaa gtagaagctt ttaaactagg ttatgatgta gaaccaaaat
    44881 ggtttattga gaatgataga gtttgtaatt ttatgcaaga aaaatgtatt aatggtcata
    44941 taagttgtga tttaaagaca ctagaaggta ctatgagagc taataaaggg gattacatta
    45001 tacaaggtgt aaaaggagaa atatatccat gtaaagcaga tatatttgaa atgacttatg
    45061 agaaagttga atatagagaa aaaaataaat tatcaacaga gatgacttta gattcaacaa
    45121 actttcaaga aaatattaaa aaagctagaa aagaattaga tttatttata caaactttag
    45181 aaaaagcaga tgataaaatt aataaactag cagaaaaaat gaataaatgt gattgtaaag
    45241 ttgatataga taagattgta aaacagttag tggaacacct aagagaaggt attgaataag
    45301 ttttggggga tggaaatatg tttaaaaaga aatatattaa aaagccaagc aaaataagtg
    45361 ttagaaatat tatagcattt attattacag ttattggtat agctttaggt gtttttatag
    45421 gtatcaacat aattatggct catgttttag gtatagctaa catggtagat aataatactt
    45481 tcacatgtgt tagattagtt tataacttag taggggttat aagtgggtat ttgataggaa
    45541 aagcaatata ccttatagct tcactaataa gctatattat ttatgaataa tttgtttaaa
    45601 ggttttttat taggtaaaaa acaaaaggag ggttgtatta ttgaaagagt atgtaatttg
    45661 gtttaagagt ggaaattgtg tatctggaat aacagacgaa tatgttgctg ataagctaat
    45721 gaaagatttt attgaagttg actcagattg taggtatttg aaagggtatt tagatgaaga
    45781 tggaacaacc gtaatagatt tatcacaaat agaagctata tcaataaata attgtaatga
    45841 gaataataat attggtttta gtaagtccta gatagggttt tttattatgc aaaaatgaaa
    45901 ggagaattaa ataaaatgaa aaaaggtgaa ctaattgcac taggtcttag tgaagaagat
    45961 gcaaagaaag tagaagttga atcgctaaaa gaattagaca attatattaa taagattgag
    46021 tatgaaaagg taaaagaaga attaaaagca tctaaagaag ccattgaagg tttcaaggat
    46081 ggaatgacaa aagagcaaat tgaagagctt aaaaaaggat atgagactaa attaactgca
    46141 aaggatgaag aataccaaaa taaattaaag gaaaaagaac aaaaagagtt tgatatggca
    46201 ttagaaaatg aacttattaa acttaatgtt catagcacta aagcagcgaa agcagaactt
    46261 gatttagaaa aaataaaata tgaaaatggt acttttacag gactaaagga acagactgat
    46321 acttggtcaa ctcaaaagtc tttcttaata aaaacaggaa agactaagat aaattacagt
    46381 cccgataatg gcaatgaaaa tacattaagt agagctgaaa gtattgctaa agaaaaaaat
    46441 gaggaaagtt ctaaaaatcc atatgctgac gcatggagta ttaaataaaa aggaggataa
    46501 agtatgtatt ttaaagaggt aaattttgat aacacacccg agtttctagc ttctcaacac
    46561 tatattaatt tttcaaaaac agcaatagat acagatgtag ttgctgatga aaatggcaag
    46621 aaatatgtgt tagcaggaag cttattaggt gaaagtggca aagtggtaaa gataactaga
    46681 gggggttctt cagatagtta tacatattca ttatcagaaa accctgtagg aatagttttt
    46741 tcaactgtag atgttactta tggaccacaa ccagttgcat caatggtgga agggtatgta
    46801 ataactgaaa gattgcaagg tgagtatgta aaagaagcta tagacactat aaagacgaaa
    46861 ttaccaaata ttaaatttat gtaggaggat gaaatatggc aagagtagaa gaattattgt
    46921 cagttcaaga gctgataaac tatacaaaga ctagaaaatt aaaagaaaca atgggagatt
    46981 tattatttcc aactcaaaag atagaaggac ttgaaataaa gatgataaaa ggtgcatcta
    47041 atcttccagt atcagcaagt gttcatgctt ttgatacaga agcagaaatt gcatcaagag
    47101 aaggtgctaa tttaagtatt gctgaacttg cacttgtgaa aagaaaaata aaactagatg
    47161 aaaaagatat aattgtactt gaagagccaa gaaactcaca agaagaaact caaatgataa
    47221 atcaaatatt taatgatgtt gataatcttg tatcaagtgt aaatactaga atagaagcaa
    47281 tgagaatgga agttctaaca acaggagaac ttaatataaa tgaaaatgga gttaaagctt
    47341 ctttaaaata tggaactcca acaaatcata aagaaacaaa aacttggtct agtggaacac
    47401 cagatatatt aggagatatt tataatatga ctgataaaat agttgttgat actggattta
    47461 ctccaacaag gtcattaacc tctaaaacta ttttaaacac gatattaaga gatgaaaaac
    47521 taagaaaagc tatatttggt gtaaatagtg ataaattgct tactttaaaa gaattaaatg
    47581 tatttttagc ttctcaatct cttcctccta tttttactta tgatgaaaga tatagagttc
    47641 aaggtaagga tggtaaatac acaacaaaga gatttttaga tgaaaataag tttattctta
    47701 tgcctgacgg caagatggga gatactttct ttggattaac agcagaggaa ttagaactta
    47761 gaaaaaatcc atcaatagac attagttcag ttggaaatat aattgtagag cagtattcta
    47821 ctgctgaccc agttgctaag tggataaaag cagttgcaac agtcttacct agttttcctt
    47881 atgctgacca agtgtttatg ggtacaataa attagaggtg ttaatatgga agttgaaaga
    47941 ctaaaaaagc ttttaggatt tagtagagaa gatgattcaa aagatacaat actagagttt
    48001 atactagaag atgtagaaga aatggtcaaa aactattgta atgtacctac tataccagaa
    48061 caattaaata gtactatttt aagaatggct atagatatgt ataaaaatga gagtctagga
    48121 agcgaagata ttgcactagg ctctatttct tctatatcag aaggtgatac atcagtttcc
    48181 tacagaagtt cagctagtga atttaaggaa tctttactta aagattacaa gtcacaatta
    48241 aatagataca gaaaacttag gtggaaataa tgatggataa gactagaaaa gcaatagaaa
    48301 tgctgtatag agataaatgc actatagtag agtatcagcc aattaaagac cctgtaacaa
    48361 aacgagctaa caataaagaa gtagtcgtat tagaaaatca accatgtaaa ctttcatata
    48421 aaaatatagt ttctgctaca gaagggaaag tagctaagct agagcaaact attaaactct
    48481 ttatatctcc agatatagaa attaaagcag gttcaaaact tattataaat gataaagagt
    48541 atgtaagaag tggagaatca gctatatatc caaatcatca agaaataata cttgagttat
    48601 ttaaggataa agcataatgg ctagatgggg cagtgttgat tttagagagt ttaaaagagt
    48661 ttgtaaaaag atggagaagc ttacaaagat tgatttagat aagttttgca aagatgcagc
    48721 aagagaatta gcagcaagac tcttaggaaa agtaattaga aggacaccag ttgatacagg
    48781 attcttaaga caaggatgga atggagtggc ttatgctagg tcgcttcctg tgtataaaca
    48841 aggaaataat tatattatag aggttgttaa tccgactgaa tatgcaagtt atgtaaattt
    48901 cgggcataga actaaagatg gaaaaggttg ggttaaagga caacatttct taacaatttc
    48961 agagatggaa ctacaaagcc aagttgataa gattatagag aaaaagttat taatattact
    49021 taaaggagta tttgatgctt aataatatta tagatggaat atcagtaaag ttagataaat
    49081 catttggaga aaaatataca atttatagtg aggatgtaga gcaaggtatt aatgaacctt
    49141 gtttttttat tgttccttta aatccaagca agacaccata tccaagcggg agagaattaa
    49201 agaaaaattc ttttgatgta cattatttcc ctcgttcaga agctaagaat tttgaaataa
    49261 atgagatagc tgagatgcta ctggaggaat tagagtatat agaaattgat ggagatttag
    49321 tcagaggtac aaatatgaat tttgaaatta tagacaatgt tcttcacttc tttgttgatt
    49381 ataactattt tactataaaa aataatgata ccaataagat ggatacagta gagttattcg
    49441 gtggtttgaa gagaggtgat aattttgaat aaaacattaa gcaaagaaga tgactacaag
    49501 tttactaagg agcaaatagt taattctaag aagtatgtaa atagaaaaga cttattaaat
    49561 gcaattttaa aagaaaatga gttatattcc ttctcagagg tagaggaaat aataaataaa
    49621 tttatgaaag gagtgagcta gatggcgtta ggtggaggaa catttgtaac acagaataaa
    49681 atattaccag gtagctatat aaattttatc tcagctaaga gggcaaccag ttcattatcg
    49741 gatagaggta ttgttgcaat gcctttagag ttagattggg gcatagatga agacgtattt
    49801 caagtaacca gtgatgattt tgagaagtat tcagtgaagt attttggata tgattatact
    49861 catgagaagc tgaaaggctt gagagattta ttcaaaaata taaggttggg atatttttat
    49921 aaattaaata aaggcgttaa agccagttgt actatagcca cagcaaaata tagtggtatc
    49981 agaggaaatg acttaaaagt aacagttaca acaaatatag atgataacac taaatttgat
    50041 gttgtaacac ttttagataa taagaaggta gatactcaaa tagcaaaagt tattacagat
    50101 ttagaagata atgactatat cacttggagg aaggatgcaa cactagaagc aagcgcagga
    50161 cttgtattta ctggtggaac taatggcgaa gctgtgacag gagcagagta ccaagctttc
    50221 ttggataaaa tagaaagtta ctcatttaat gctttaggat gtttggctac aacaacagag
    50281 attaaaagtt tgtttgtaga atttacaaag agaatgcgtg atagagttgg agcaaagttt
    50341 caaacagtat tatataaaaa gaatgatgca gattatgaag gtgtagtatc tgttgaaaat
    50401 aagattaaag atactggatt attagaatct agcttaattt actggactac tggagctata
    50461 gcaggatgcg atataaataa atctaatact aacaagcgat atgatggtga atttgatgta
    50521 gatgttaatt acactcaaat acatttagaa gaagctttaa aaactggtaa gtttatattt
    50581 cataaggttg gagatgaagt tcatgtatta gaggatataa atacttttgt tagttttaca
    50641 gatgaaaaga atgacgactt ttcaagtaat caaagtgtta gagtgcttga ccagattgct
    50701 aatgatatag cgactttatt taatacaaag tacttgggtg aagtaccaaa tgataaatct
    50761 ggtcgtatct cgttttggaa tgatgtagtt aagcatcatg aacaactgca aaatatgaga
    50821 gcaatagaag atttcaaagc tgatgatgtt tctgtagaac ctggaagcga caagaagact
    50881 gttgtagtaa gtgatgctgt aaaagttatt agtgctatga gtaagcttta tatgactgtt
    50941 tcagttagtt aacaataaga aaggagaata ataatggcac aacaaataaa agcaagagat
    51001 acaataagtg catctaaggc agagtgtttt gtaactataa aaggtaaaag atataatttt
    51061 atgcaagcta ttaacttaga agctaaaatg gaaaagaata agagtgagat acctatatta
    51121 ggtagtacta caaaaggaaa taaatcaaca ggaagtaaat attcaggaaa tgcaacattt
    51181 tattataata cctctatatt tagagaattg ttgtatgagt ataaagaaac tggtgaggat
    51241 atttacttcg atatacaaat taccaatgaa gacccaacaa gttcagtggg tcgtcaaact
    51301 ataatactgg aagattgcaa tatggactca ggcataattg ctaaatttga tgctgatggg
    51361 gagtatttag atgaagatat ggattttact tttgagaatt ggaaattagt tgagaaattt
    51421 aatatagcaa atggtatgga gtaaaataca catttatgat ttatgtatgt gtatttttta
    51481 tttataagaa taggagatga ttaaaattaa ggataaatat gagataaaag attcaatttc
    51541 ttttgattat agcaataaaa gacctttgga agaacgtgtc agcgagatgt ataaaaaggc
    51601 aggaaaatat cttatagata tttcagataa gttagcaaca gatacaattg atggttcgtc
    51661 attaaagcca ataatcataa aatttgaaat aaatgaagct ggtgttgcaa caatagaaaa
    51721 acaaacaaaa tatttggtga tggaggtaga ataagaatat gggagattta aacgcttttt
    51781 taagtcaaaa tgcaataaaa gtagagaata gaaagtatgt ggcaagtgaa aggtttatag
    51841 gagaagatgg aaaagcaatc gaatgggaac ttaaagcaat agattcagat agggacagac
    51901 aattaagaaa agattcaact ataagagtac ctatattaaa taaaaaaggg aaagctacag
    51961 gacaatacac tagtgaaaca gattttaata cttacacttt gaaattatgt gtggaaacta
    52021 tagtatttcc ggatttacat gatgcagaat tacaaaatag ttatggtgta atgggtgcag
    52081 aggagttatt aacaacaatg ttgactcctg gcgaatatac agacctttca agtgaggtag
    52141 gagaggtgaa tggttttgat aggacttttg aggataaagt agaagaagca aaaaactaat
    52201 tgaaggaggc gattatgatg ctagtgtagc tcattattgc cttcataaat tcaaatggaa
    52261 accacatgaa tatacagatt taccagactt cgagagggca tttgttgctg cttctataga
    52321 tattaaagta gaagaagaaa taaaagaaga aaaaaagatt gctaaagaag ctagaagaag
    52381 tagaagaaga taaaatatag gtaaaatatg taagaattat atgttataat aattgtagca
    52441 agaagatgta atctacaatt tatagagtgg agttcatact gggataaaac ctacttccta
    52501 atgaaaggag gtgggaagta tggacaattt tttacttagt atattagcta gcttgatagc
    52561 tagtttaatt ggatatatcg tttgtagatg tatcaaaaac gtaaaaagcc actctactcg
    52621 tggcaagagt aaaagtggct gggaacttgg ttttaaaata aagttccgca aatttaaata
    52681 atttatattt tttaaaatta tgaacttcac tctaccgcta aatagattgt agttcttctt
    52741 gcctttatta taccacaaat tagaaaaaat atgcaaagta cttgttttaa tagtaagtac
    52801 tttttttatg tgaaaaagaa ggtgattgaa tgaataaaga tatagaactt acagaaaaag
    52861 atttatattg tattgctaga catattcaag tgtatgtgtt taaaaaagat gatgaggtca
    52921 taaaaaagga agataatcca tgttgcagat gcaagcataa atttaagtgt tatgagaata
    52981 gtgtatgtat ctatcattgt tcagtatttc agaagttatc taaaattaca ggtcttaaaa
    53041 tgggttttgg agtcaaacta taatgtaaaa attttgaata tatagtataa tattcttata
    53101 aaatataatt tagggggata ttatgagaga aggaaagaga agaagaggat gtctattttg
    53161 gtttatcatt attattatat tttctggggt tgttggagca atagcaggga acagtactaa
    53221 taatggaagc actgaaaaac aaaaagaaga tttaactaaa tatattggtg aagaaggtaa
    53281 tataggagat ttaaaattaa ctgttaatag tatttcaaaa gctagtgaaa taccagtagc
    53341 ttctggttat ttggcgtaca ctccagatag tggcaaatat ggtattataa atttaacaat
    53401 aaaaaatcaa actaaggaaa gccaatcttt tatgctaaat tcattcacat taataggacc
    53461 agataattca aaatatgttc catctttatt gattgatgta ggaaataaat atataactat
    53521 ggatactgta aatcctaatt tagatgtaac aggtaatatt gcatttgaaa ttcctaagga
    53581 tttactagtt tctgattgta aactaaaata tagtggttca gagcaagaat ttatattaaa
    53641 agaaaaataa tatatagatt taaaatcaaa aatactaaaa acacttacta atgtaggtgt
    53701 ttttttaatg aaaggatgtg atgataatgt aaaaatttta ctaatatagt ataatattct
    53761 tataaaatta ttatactggg ggggaaatgt tatgttttgt tcaaattgtg gtgcagaaat
    53821 cacaggcata ggcaagtttt gttcaagttg tggggttgct gtagaaactg aaattattga
    53881 agataataat attaaatcaa atgacttaat cgttgatgct aatggaatag aaataaatat
    53941 gactgaaatt tatagaaaat atagaaaaga aaaagtaaac gcaataaaaa atgtaatgga
    54001 aataagtggt ttgaatataa aggaagcaaa aaaaatagtg gatttttctt ttgaagaatt
    54061 aaaaattaat tttattgatg atactatgag taattcagaa aaggaaaaaa taatacaaaa
    54121 tcaaaataga aaaaataata ttgaaaaagc tcaacaagaa tcagttgctt gttgccctaa
    54181 gtgtggttca acatccttga cagctcaaaa aaaaggtttt ggtataggga aagcaatggt
    54241 aggagctagt ctaactggtg gtataggttt agtagctgga aatttaggag caaagaaagt
    54301 tagagttaca tgcttgaatt gtgggaagca gttttgggca ggcaaaaaat agatgtatta
    54361 attataaaaa cacttactaa tttagtaagt gtttttatta tctaaattaa cagaaaggag
    54421 agtgaaaaaa tggctacaat acagacatca attcgcatat ttgatggaat gacacctgca
    54481 tttcggcata tgacaactgc tatgaatata gtactgagtt catttgagca attacaaaga
    54541 acttctagta atgcagtaaa tgctaatagc ataataagag ctagagaaga actagcaaga
    54601 gcagaagctg gatttgatag attagaacga caaataagag aatcagataa tcaacagcga
    54661 aaacttaatg aggatataaa taagggtgca agttctacag atagattagt tggaagtgca
    54721 aagaagctag cagcaactta tttaggtata agaacattag gaggtctagg aaatttaagc
    54781 gaccagatga caagtactaa tgcgagactt ggcatgataa atgatgggca acaatcagat
    54841 gctggactta ataaaatgat atttcaatca gctgaaaggt caagagcatc ttacttagat
    54901 actgcacaaa tagtgagtcg tataggcatg aacgcaggaa aggcgtttag cagtacaaaa
    54961 gaaattgtag gttttgcaga gcaattaaac aaaaaatttg taatagcagg cgcaagtact
    55021 gaggaaatga actcggcatt gttacagcta acccaagggt tgagttctgg cgtattaaga
    55081 ggtgaggaac tgaatgctgt gtttgagtca gcacctaaca tcatccaatc gattgcagat
    55141 tatttggacg tggacatagg aaaaataaga ggaatggcat cagagggaat gttaacagca
    55201 gatattgtaa aaaactcatt acttgcagca gcagagcaga ccaatgcaga gtttgaaaaa
    55261 atgccttaca cattttctca aatttggact tcaattaaaa ataatgcaat catgatattt
    55321 ggtgttatac agaaaaaaat tgaacagtct atgtctagta agggatttcg aacctttata
    55381 gataatttta taaactcttt gtatgtactt ggaaatgttg cttataacat ttttaatgaa
    55441 attataagta tattagggag cccgtttttt caagcatttg taaatgcgat tattgtaggt
    55501 gttagtttaa tagtgcaggc actaggttgg ataataacac aggcattaaa tattgctaat
    55561 gtgtttgctc agaactggag tattattgca ccaatagtac ttggagttgc ggctgctatg
    55621 ttagtgtata acaatgcgtt attacttagt attgcgaata aagttaaaga tattgcacta
    55681 tctgccaaat ctttagtcat gagctttgca catatagtag cagagtctta tagagcagca
    55741 gctttagtag caactacaat agcacaagac ggattaaatg cagcaatggc agcttgtcct
    55801 attacttgga ttttatatgg aattatagct atagttgttg cattttttgt agctatagca
    55861 atatttaatc acttcgcagg cactagtgta tctgctatag gtgtagttgc aggtgcaata
    55921 tcagttgcag cctctttcat aggaaactta tttattgcaa caggaaattt aattatagat
    55981 attgtagctt taatatacaa tactttagca ggctttgcag agttctttgc taatttttta
    56041 gacgacccaa ttggctctgt tataagagca gtatccggaa tggctaatgc tgtattaggc
    56101 attataagaa gtatcgcaag tgcatttgat accgtgtttg gttcaaactt ggcagatgca
    56161 gtaagtggat ggcaagataa attgcaaggt tggactgata aagtagcagg agaagctaaa
    56221 ataaaagttg aaagaatgga ccctaataaa ctgcattttg acagatttaa ttatggaaaa
    56281 gcatgggacg caggatataa atggggagat aagttagaaa ctaatataaa agataaattt
    56341 gatattagca aaatggcaga agatgcaaag aaaaaattag gattggatga tttatgggat
    56401 aaaaaatatg gattaggaga tggatttggt tcagcaggat taaactctcc tctcaatgat
    56461 gcagcaaaag gagcaaaaga cacagcaggc aatactgcaa aaatggcaaa gactatggat
    56521 aaaagtcaag aggacttaaa atatcttaga gacattgcag agcaagaaac aataaaccga
    56581 ttcacagggg taaatataaa aattgacatg aacaacacta ataacataag taaagatgca
    56641 gatgtggatg gtatagttaa tgtactaaca gaaaaactga atgatgctat ggttgtatct
    56701 gctgagggaa tagtttagag aggaggtgta atcgtggctt atgattttta tttagatgga
    56761 gtacaattac caatacctcc accaaagtta gagattaaag ttacaaataa aaataagaca
    56821 gttgatttga taaatactgg agaagtaaac atattaaaaa aagaagggct atctgaaata
    56881 agttttgaag cagagtttac acataataaa ttaccttttt atcgtggaac ttttagggat
    56941 gttcaattct ttttaagtaa gctagaatta ctaaaaactg attgtaagcc atttcaattt
    57001 attgtatcga gggaaatggg tggaaaagta ctatttaaca ctaatatgaa agtgtcatta
    57061 gaagaatata acatagtaga agatgcagac aatggttcag atgttaaagt tgcaataaag
    57121 ttaaagcaat atagagatta ctcaactaaa aaattagttc ctgcaactcc tgaaaagaca
    57181 aactatggta ggactcccac tccagtcatg aaaccaaaag aatttagacc agattcatcc
    57241 aataagccaa atggtaaaac atatacagta aaagcagggg atagcctttg gtcaatctgc
    57301 cagaagcaac ttggtaatgg ttcattatat aagaaagtat atgagttaaa taaaacaatg
    57361 atggataaag ctaacaaggg taaaaaagta cctaagtata ccatctacaa agggcaggtg
    57421 ttgaggcttg tctgatgaat tagtattagc aaatgatagg gatgtaagat tagttattgc
    57481 acattgggaa gacttttacg aaccagttgt tttagatggt attacatggg agatagaaag
    57541 acgaggtaca ccaagtaaat tagagtttac tatagtaatg gatgatatac tacagttttg
    57601 tgaaggtaat tcagtgagat tatattacaa gggtataggt atcttctatg gatatatttt
    57661 tcaaaagaaa agagacaaag aaaatcacat taagatagtt gcttatgacc aattgcgata
    57721 ctttaagaat aaagatactt atgtgtatag taataaaaca gcaagtgaac ttgtaaagat
    57781 gttggctaaa gattttaatt taaaatacaa tgttatagaa gatacaaagt ataaaatatc
    57841 tagagttgaa gaaaataaaa cactctttga tatggtttta actgcattag atgatacttt
    57901 aagacaaaaa aaggaaatgt atgttttata tgatgatttt ggaaggttaa cactaaagaa
    57961 tgtggcatca atgaaattag atacggttat gaataatgat gtaatagaag attttgacta
    58021 caattcatca atagatagcg atacttatac aaaaattaaa cttgtaagag acaacgagga
    58081 gtcaggaaaa agagatgtgt atattgctca agactctaca catataagga gttggggaat
    58141 acttcaaatg tttgatacag tagatgataa cttgagtgaa gcagaaataa aacaaaagtg
    58201 tgatatactt ttaaaattat acaataagaa aactaagtca ttaagtttaa aaaacgcaat
    58261 tggagatatt agagtaagag caggttgttt agtacctgtt tttttaaatc taggagatat
    58321 tgagttacaa aattatatgc tagttgagaa agtaaagcac acatttgaaa ataacagtca
    58381 ttttatggat ttaacacttg ttgatggcga cgaatttgct tcttattctt caagttcata
    58441 tagtagtgga aatagtaata ataaagatga aaagaaaaat ggtcctgcac aaagtactac
    58501 aaataaagaa gacagtgata tgataaataa attaaataaa gtatttaaag gaaaattatc
    58561 aaatacagga agtatatttg ttaaatattc taatgcttac aaagtcaatg cagctttaat
    58621 ggctgctatt tctatacatg aaactggtaa tggaagttct tcactttgca aaaataaaaa
    58681 taatttcttt ggtatgaaag gaatgtcttt tggctctgta gatgaaggaa taaaaagagg
    58741 tataagtaat ttatcaagaa attatattca tacaggaaga aaaacattag aaagtattag
    58801 agacaaatat gctccgcttt atgatagtcc tcttaacaaa gattgggtac caggagtagg
    58861 aaagttctat aaacaaataa caggaagtgc atatagttct aatagtgcag gtacaggagt
    58921 tggaagtaat gaagaagcag aaaagaattt aaaagatata acttatcaag ttcaaggtaa
    58981 taatcaaaat agtaatgcaa ataattctaa agcagataaa ttaattagta tagctaaaag
    59041 taaattaggt tgtaaatatg tttggggggc agaaggacca aatacttttg attgttctgg
    59101 atttacgcag tggtgctata aacaagtagg tataaaaatt cctcgaactg ttgcaacgca
    59161 gagtaaagca ggtaaggctg tagatttaaa agatagaagt aaatggaaag caggagactt
    59221 attatgtaga gttggtggag gaaatagtaa tcatgtcatg atgtatattg ggaatggtaa
    59281 aatgattcat tcgccacaga ctggtgatgt agtaaaaata caatcggttg attcatatcg
    59341 aaaaggaaaa gcgtacacac atgtaagaag atttatataa gtgaggtgat aaagtgtcac
    59401 aagacttatt acaagtgata aaaaaggctg caatggatgc agtagaaaca agcaatccaa
    59461 tgatgattgc atttggaact atagaaagtc ttaatccatt agtagtttat atagaacaaa
    59521 aagcatcttt tgatgaattc tttttaatac agacagaaac atttaaaaga tatacagata
    59581 aaaaaatagg agataaattg gttttaattc gtatgcaagg aggacagcaa tatttgattt
    59641 tagataggat gtgataaagt gttaccaaca gataacattg actatgacat tgaagatgta
    59701 tcaataatta actttgatgt aaggcaagaa ccaagtaaga catttaaact tcatatagaa
    59761 aaaaacagag tagatggtat ttgtgatgat gtagaagcat taaaacaaac catcttttta
    59821 attttaaata ctgaaaggta tgagcacctt atttattctt ggaactatgg agttgagtta
    59881 aatgacttaa ttggagaacc tatttccttt gtaatacctg aaattgaaag gcgaatcaaa
    59941 gaagcactaa ttcaagatga taggattgaa actgtagata attttgagtt tcaaaatgta
    60001 aaaggaaagg tccattgtaa atttatggtc cataccaaat atggaaatat caaagcagag
    60061 aaggtggtga gtgtataatt gtttgagtta atgacttttg aaaatataat taaaagaatg
    60121 ttagatagtg tacctaacac acttgataag agagaaggtt ctataatata caatgccttg
    60181 gcaccagttg cagtggagct tacagaaaca tatattgcta tggatgaatt actagaccaa
    60241 acattcgtag atactgctag ttattactat ttagagaaga gatgtaaaga gcgaggaatt
    60301 acacctttac cagccactaa tacaattgct aaaggagttt ttaacataga tattccactt
    60361 gattctaggt ttaatctagg agaatacaat tatattgcaa ttgagagaat atctgaaaaa
    60421 acatataaaa tgaaatgtga aactgctgga cctatttttg agttagggca attaatacct
    60481 attgaatata tagatggtct tgaaactgct gaactaactg aaatcttgat aaatggagag
    60541 gatgaagagt cagaggacag tctaaggcaa cgatattatg atagtttaaa ttcacaatca
    60601 tttggtggaa atatgcaaaa ttataaagat gaagttaaca aaatacaaga tgttggagga
    60661 gttaaggttt atcctgtgtg ggacggtgga ggaactgtta agttagtaat aattaattct
    60721 aatttcaaag taccatcaga ggatttagtt aatttagtgc aagaagaaat tgacccaatt
    60781 ggacatcaag gacaaggctt aggattagcg ccaattggac accgagtcac agttgaagga
    60841 gttacaagta caactataaa tatatcagca gaaataacat ataagagtgg atatacatgg
    60901 gagaatataa aaacaattgc agaagaagca gttgatgact atttaaatga acttaatatg
    60961 agttgggaag atgaagaaaa cttaatagtt cgtatatctc aaattgaaac tagattactt
    61021 agtattgatg gagtattaga cattgcaaat acaatgataa atagcattgc atctaatctt
    61081 aatgttgata aagattctat agtattaaga ggtgaagtag ttggataaag agattaatct
    61141 aataaattac ttgccacaaa ttctacaaga taaagaagaa tatataaaag tatttaatgt
    61201 agaaaacaaa gaaataaaaa cactacatga caaattaaag gacctatcaa atgaccagtt
    61261 tttagaggat ttaactccaa gtggtgtgaa gagatgggaa aaaataatgt ctataaatcc
    61321 caagtcaaat gagactttag aggatagaag gtttaggatt tttagtaaat atataagtaa
    61381 actaccttac tcagagagat ttttaaggaa ctggctagat aatgtagttg gagaaggcaa
    61441 ttatgaatta actattaata atgctactta taacatacac cttgagagtg atgctagaaa
    61501 tcaagattgg tttgaggaag ttcattcttt tgtaagtaat attaagccat gtaacatgac
    61561 actagattac actagagtgc ttataagcaa agacaattat atgaattttg gtataacaac
    61621 cttaatgggt caagaaataa ctatataccc ttggagtcca ccagatatag aaacttatgg
    61681 agaaattgat gtattaactg gcaatggagt tggataccaa gagataacaa tattttagga
    61741 ggtgatatat tggctataga taaaagttat tacactataa ttacagatgt aggaaaagca
    61801 aagatagcaa atgcaagtgt cacaggtaat aaagtgggat ttgtaaaaat tcaacttggt
    61861 gatggaggag ggagtgaata tactccaact gagagtcaga cagctctcaa aaatgtggta
    61921 tgggaaggca atatcggaaa tacaactaca gatgaaactg caccaaattg tataatatta
    61981 gagagtttaa taccatcaag tgtaggcggg tttatgataa gagaaatagg atatttagat
    62041 gatgaaaata atttaattgc catttctaaa tacaaagagt gttataaacc ttctatagaa
    62101 caaggtgcag tggtagacat gaaggttaaa actgtgctta ttgtatctaa tgtaaataat
    62161 atagaactta aaattgaccc aacaataatc tttgcaacac tcaaagatat acaagactta
    62221 gaaactaaaa taggtactgt taatactaaa attgatacaa ctaaaacaga attaacaagc
    62281 aacatagaaa ctactaaaac agagctaaac actagaattg acacagaaaa tgagaaacaa
    62341 aatattaaaa ttgaccaact tattgcaggt ggctctaatg tggcatctac tcaaacaata
    62401 acaattgacg attgggttga ggatgcagaa aatggattca aagcaactgt aacacatagt
    62461 ttattaacac agagaatagt tgtaaatatt atagatgcta ctacaaaaga aaatgtagtt
    62521 acaaacttta aaattataga tgataattct atagaagtta gaagtgaaac aaggtcagaa
    62581 ttaaacgttt atgtgataaa tggaaatgca gaaactcatt ttataaatgc gactgtagat
    62641 gataacagag tatctgaaat gactacttat tcgtctaaga aaatcgaaga tagattggtt
    62701 aatttagaag aaaaagtaaa tggtggttta tctagtattg caacaagtgt aaatgagttg
    62761 ataacttatt gttagaaaag agagtgataa aatgcaaact gaatggaatt ttgatgctgt
    62821 aagttcagca caagaaatta tacttaaacc tggtaaatat aaatttgaat gttggggtgc
    62881 taggggtggt gcattaggaa ctcctttcga aagtggtttt tattatggta agggtggtta
    62941 ttgtagtggt gaaataacat taaaaaaaga aactacttta tatctttatg ttggtttaga
    63001 tggcagaaaa ggttataatt tcaatggtgc tggatatggt aatggtgcaa gtggtggtgg
    63061 tgctaccgat attaggctta ttggtggtac ttgggataat gaacaaggtt tactatctcg
    63121 tataattgtt gcaggtggtg gcggtggaac ttatgataaa caacatggtg gtgatggtgg
    63181 tggcttgaaa gggactcttg gaacttcttc tactggtgct gctgctcatg gtggtacaca
    63241 atttgagggt gggcgaggta gagacaaaga tggttcttgt gatggttttt ttggtaaagg
    63301 tgctactcct gaaaaccctt catcacaatc tggtggtggc ggtggttggt ttggtggtgc
    63361 ttatcctgct agtggatttg gtaatggaag tggcggtgga agtggttatg tactaactaa
    63421 agatagttat aagccacctg gatatacacc aacttctgaa tattattttg ataatgtagt
    63481 catgacaact ggtggaaata ctactgttgt aggtaattac tcagatggtc gtgctaaaat
    63541 aacattgcta caagcattgc catttttaac agtatcttct tataattcta cacaagcaac
    63601 atttaaagcc gaccacacag accctgcatt acttacaaag atagagtggt ttatagatga
    63661 tgtactaaaa gaaactataa caaccgattt aactcttgaa aaaacaatta actatacatt
    63721 agaagataat gctctacaca cacttaagat agttgttaca gacagtaata atgctacagc
    63781 agaaaaagtg ttaagtataa gtaagaatat aatgccactg cccgaagaaa atgtaaattt
    63841 aaatgatata tcaacaaaat tagttgaggt taatgcagga tttaaagttg ggaaaacaag
    63901 tattataaat actttagcat taaagaatat agaagcaagt ttaaataata cacttgtgga
    63961 gttatcagag aaaataaaaa caagttttga tagtggagat gctagtgtac aggatttgat
    64021 gaatcagtta acacaagcta ataatactat atctcagtta aatgctaaat ataaggttgc
    64081 tggtggttgg actactcctg tttatacaaa tggtactgaa aaagctcttg tatataattc
    64141 aactcgtgag gctatacgtt atgattggat tactatttca aatttaggtt tcattcctaa
    64201 tattttttat gctgaatgtg attatgttaa tagctatact aaaagtaaaa acaaactttt
    64261 tgtttttgca tgttatgatg tgcctacttc ttctagcaaa gattttgttt ttacttgcga
    64321 tatacaatta ggaagttctg atagtgaata taaagtcact tattgtggcc tttatcagca
    64381 taataagctt gatattcata tgactgataa tctcattcat ttacctgctt ttgctactat
    64441 taatgctgtt gccagttatt actggcgtgc tattaaaatt tattaaataa attaggtggt
    64501 gataaataaa atgaatagag caaatagaat tatatgtgac caaacaggca aaatactctt
    64561 gcaaacagga gaagcaacag gagatatatt gcaacatgat acaataactg aattacattg
    64621 tattgatgtt gagtatggaa gtatagacta tactaaaaat agaattatag gtataaatat
    64681 agaaactaaa cagccaattt tagaagaaat accagtatat ctaacagaag aagaaaaaag
    64741 aatacaagag ttagaaaatc aaatattact aaatgaaaat gaaaaagtag gaggtatttt
    64801 ataatgaata taaataatgt tgtagtaaga atattagcag agaggatttt aagtagaggc
    64861 ttaaatcctt taaaaaatcg agaatttgaa ttagatgatg taactaacac agactatagg
    64921 acagcagtag aggattatat aattaaacag agtggagtag tggaaggaat agaaccaaca
    64981 gcgtagtagg ttcttttttt attgaaagaa ggtgattaaa tgacttttaa ggagttagtt
    65041 aataaagtta gaaatcttgt attagaagca aagaatgtaa ctatagaaga tacagaaagt
    65101 aaatttacaa gtgaaaatgt agaaggagca ttgaaagaat gtatagatag agcagatgag
    65161 gcttttcaag aagccgatag tggaaaaaca cttttatcaa ctgctatcgg ctctcccgct
    65221 acatcagaac aaacatttca agattatgcg aactatatta caggatttaa gagcaatata
    65281 agcaatttag aaactcaatt gaaaagcaaa tattctatta gacatggtcc cattgatgga
    65341 gatgatagga atcctttttc tgctaatttt ggcaagagtg caagttacct tattgtctat
    65401 gtttacttta gaagaaatgt atattattat aatcccagtg gtagttcttt aggaagtagt
    65461 acaggaggtt ctgaacgtgc ctggattact atagatagca ataaaactgg tttttcagtt
    65521 cattcatatg atactaatta tgagtcatat acttttacag gttattatat tgcttgtttc
    65581 gcataataaa ttatattatt aaaactagga ggatgtatgg aagaaattag cataaatcta
    65641 ttatgtgcag ttgcaggagt tgtaatatcc tacttagcat ttagaaatag ctcaaacaga
    65701 aagatacaag atgatacaga aacaactaca aaattggaac aacaaataac ttttctgtgt
    65761 gagaatgtaa gagatataaa gcatgatgta gcaaagttta atacaagttt cttagatatc
    65821 agtgaacgag ttgcaaaagt agaagcaagt acaaaacaag cacatcttag aattgatgaa
    65881 attataaata gaattggagg aaaataaaag atggataatt taataagttt tataccagag
    65941 caactgcttt tattagtagt tgctcttaat gtattaggat ttggatttaa gaaatacaaa
    66001 cagttagata ataaatacat tccaatcata ttgcttgtgc taggaattgg attttcaata
    66061 tggatgctag gatttaaccc ttcatcaatt ttacaaggta ttttatgttg gggagttgca
    66121 ataggagcaa atcaagttta caaacagttg aaggaggaaa acaaataatg aaaataggta
    66181 taaattgtgg acatacaaaa acaggaccag gaagtggagc tataggtaaa ataaatgagt
    66241 caatagaaac taggaatgta ggatataaag taatagataa attaaaaaaa ctaggcaata
    66301 atgtagtcga ttgtactata gataaagcgt ctactcaatc agaatgttta tctaagataa
    66361 cagcacaagc taatagacaa gatttagatt ggttcatatc aatacatttc aatgcaggtg
    66421 gtggcaaagg atgcgaagtt tacacatata aaggtaagca gtatcaagat gctatagatg
    66481 tttgtaaaaa aatttctgat ttaggattta caaatcgagg ggtaaaagat ggaagtggat
    66541 tatatgtagt aaagaaaaca aaagctaaaa gtatgctaat agaagtatgc tttgtggaca
    66601 gtgaagatgc aaataagtat ttgagtttag gagctgataa attagctact gcaatagtag
    66661 aagctataac taaacatata agttcagcag aagaaaacaa ttataataga tataaacata
    66721 caatagtgta cagtggtgat gataaagtat cagcagacat tttaggacta tattataaga
    66781 gagaaaaaga aagttactta gtaacagata taaaagacta taaaccacat agaacacaaa
    66841 atttgtacgt aattggtgga gtaacttgta ataaaatgaa ggaaatgagt aagactacag
    66901 gagaaaaatt tactcaacta tatggtaatg atgtatggtc aacaatggat aaagctatag
    66961 aatttgtaaa agaaaaattg tagagttaaa ggtgagaata tgttaataca agagaaaagt
    67021 ttttatccaa acgatattta ccccaaaata gactttctca atataaaaag gcagttaaaa
    67081 tcaatataca agaatgattt atcagattgt gggagcatat gtataataga aagaaaagac
    67141 tattctatgt ctgtaaacag tctaggtgaa gtaattatat attatgattc aaagtttaag
    67201 caatgtgttc aagatgcaat aagagatatt gaattaatgt ttaaaagtca aattagaagt
    67261 ttttatttga tagatagatt agaaggtagc aactagttag ttgttacctc cttttttaca
    67321 cctcaattaa aacagttttt ataaaagata gtattttaca aataagtaaa attgataatt
    67381 caagttaaag atatttactt acattttgcc tacactttat atacaatttc tagactattt
    67441 tgtaattcac ataatatata ttaaatgtaa tata
//
LOCUS       DAELLV010000002        63866 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.002, whole genome shotgun sequence.
ACCESSION   DAELLV010000002 DAELLV010000000
VERSION     DAELLV010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 63866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63866
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.002"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..2066)
                     /locus_tag="KPD58_000101"
     CDS             complement(<1..2066)
                     /locus_tag="KPD58_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBG0263897.1"
                     /translation="MVELIITTNIDIRKLNENQLEAVEHIDGPCMILAGPGSGKTRVI
                     TYRIANMVVNKNIAPTRILAISFTKASSIEMKNRALNLSDDIRLNKVTYGTFHSVFFK
                     ILRYFERYNLDSIFDEKSKRMTIKAILKSLNIENADDDENIGQVINEISYVKNELMDK
                     NEFNSEVLTKDEFLKVYNLYEEQKSKVNKIDFDDMLIRTYYLLLNNKSALEMVRNVYK
                     YILIDEFQDINKVQFEVLKLICSPLNNIFAVGDEDQSIYGFRGARPDFLLEFEQYFNN
                     TKKIILDINYRSKSEIVHTANRLIDKNKNRYEKIIKCSQGDGGSVTYISPHDSEEEAL
                     YIAREIIDEIQKDYVEYSDFAVIYRTNIQSRALVDVFMDMRIPFVVKDSVITIYDHWA
                     SQDILAYLRIGINPKSNKDWLRIINKPFRYISKDSINMVKDEKDFITALINKCNLHPK
                     QVKTINDLEIDLSYLNTLNPKNAISYIRTSLDYDRYVLDYCSNRKIKTNGLVEILNEL
                     ESSATNFNTVTEFLEHIDRVKTELTENNKNKQTEGVIFTTMHSAKGLEFRNVYIIGVN
                     EGTIPHEKSYDICKEEKKEEQLEEERRLMYVAITRAEEKLCISSTLNKYGKKVDKSLF
                     INDIKSPTKKEIDSIGVGDKVYHKKFHEGEIIKKDGIMFTIKFKDRERILDLKTCLLK
                     NIIYTI"
     gene            complement(2143..5172)
                     /locus_tag="KPD58_000102"
     CDS             complement(2143..5172)
                     /locus_tag="KPD58_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG0263898.1"
                     /translation="MENNLRKNNSIMKKMIFPLIIVMLIQTSLFCATILWGGTIKKLN
                     DNSFDILNERVINRKNYIQNEMLQRWSNTSETEATINSSVKKVLNEKNALIEDVGNNE
                     EINKDIIKSISNDLIYLLRKNSVTGAFIILSDKNINDKSIDSKGEISRTGLYIRDLDP
                     KFNPNDNSDLLIERGSSDIARSFGISMDSYWEPKFSFKSENEQDGDDFFYKPFRAAID
                     NPNVSSSNLGYWGSKFLLTNAGDRLMTYTVPLIYEDGTVYGVLGIDLSVNYLRELLPY
                     SEINGNKKGNYILGVDRNNDMAFENVISNGYSLKEKFWDGLHSNLEQKESYKNIYKIK
                     NKDNECGNGNIYASVQYLDLYNSNTPFENERWALLGIIEEKSLLSFSKRVEKLVIISI
                     AISLLIGLIGIYIASRLFTKPIVLLANKVRNSNPSKPVNLDKINISEIDELSSAIELL
                     SANVADSSSKLSQIIEMVDMPIGAFELYKNAENVFCTKEFFSVFTTEIKFEEYGYMEK
                     NKFLKKLRGLDKYLQESYETENTYIYKFESKDKKLKWIRMNIFEDELKVLGVVVDVTK
                     EFIEKRKIEYERDFDILTNLLNRRAFHSQIQEKFKNKDELKIAAFIMWDLDNLKYIND
                     TYGHDAGDEYIRCAADILKKFISWNGIVSRLSGDEFYVFIYGYESKDSIRDIINSVKK
                     QMNNTLLKLHDGTEFKIRASAGISWYPDDSTNYDELMRYADFAMYQIKNTIKGEVSEF
                     DINIYNKDSFLLSNREELNKLIDGQLVEYAFQPIVDCKGKVFAYEALMRPKIKTFSSP
                     IDVIRLARSQSKLYQIERITWFKALKSFKKYKEKFGDCKLFLNSIPNNNLSKVDFEEI
                     EDLYGEYLNRVVLEITENDKINEDFIEKKKAYIDHWNIEIALDDFGTGYNGDAVLLYI
                     MPDYVKIDMSIVRGIDKDENRQKILKNLISYSNERNIKVIAEGVETKGEMETLINLGV
                     DYMQGYYISKPSFIPEDISDKIKKELKICHENRNDVV"
     gene            complement(5225..6685)
                     /locus_tag="KPD58_000103"
     CDS             complement(5225..6685)
                     /locus_tag="KPD58_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBG0263899.1"
                     /translation="MNRCKFAKLIITSMIVLLLTGCSLKKEDNYKLDPENPTSIQIWH
                     YYNGQQKMAFDKLVKEFNDTVGAEKGIIVEAFSQGNVVDLSEKVLDSANKKVGSEDVP
                     NIFAAYSDTAYEMDKLGLLVDMDKYISKEELNEYIDEYVEEGRINSKDEFKLFPIAKS
                     TEILMLNKTDWDKFAKATNSNINDIKTIEGLVETSKKYYEWTDSLTKEPNDGKAFFGR
                     DALANYIIIGSKQLGVEIFSVKDGKVTLNIDEKVMKKLWDNFYVPYINGYFASYGKFR
                     SDDAKTGDILALVGSSSGAAYFPDRVMLNDNESYNIETAVFQAPRFKGEGKYAVQQGA
                     GMAVTKSTKEEEYASVEFLKWFTDKEPNIKFSIASGYLPVKKKTNDLDVIKEVMKKDN
                     LSLSDKVEGSIASSIEQINSCNLYTNKAFDGGTKARQILEYSMLDKAKEDSNKINELV
                     KSGTSKKDAVAQFNNDKNFKTWFESFKAELENAIKK"
     gene            complement(6777..7151)
                     /locus_tag="KPD58_000104"
     CDS             complement(6777..7151)
                     /locus_tag="KPD58_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0263900.1"
                     /translation="MGNKNKNTKYKDKTRERYNKKVENLNKEKLNKVNKQSKKVDKID
                     DIINKSSIVLCVLLVVFSLLYLFKLVQLNIIMILLVITVAIQQGLVSFGAFRNKNYVK
                     ALFSIFVIVCFLIAFKYVVNPV"
     gene            complement(7327..8067)
                     /locus_tag="KPD58_000105"
     CDS             complement(7327..8067)
                     /locus_tag="KPD58_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBG0263901.1"
                     /translation="MLYDEDEKEENNERWLLTYSDLITLLMIFFVIMYSMSNVDAEKY
                     KQLSQSLNSAFGGSSGVIEGGNSKIEPVVEPGSNDLDTLQNAKFKKVGEEIQKYLNEN
                     GMANSVSLRVQDRGLVISLKDTILFDTGKAIVKDNSRDKIIQIGKMLNEMNSYMRVEG
                     HTDNMSIKNSEFKSNWDLSVMRATNVVQLLIDNAGIAPDKLSAVGYGEFRPIAENSSE
                     EGRSKNRRVDIVLVDSKYDNVENVSKDK"
     gene            complement(8088..8876)
                     /locus_tag="KPD58_000106"
     CDS             complement(8088..8876)
                     /locus_tag="KPD58_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HBG0263902.1"
                     /translation="MTTLIFFIVGIASLVVAFVIDGGHVGALFVFSSALVVFGGTIGA
                     VGASTPFPVFKKSIKIMLLAFKNKKPNNIENIIYFKEIATKARKEGLLSIDKEISEND
                     ELDPFIRKGLELMVDGVDAVTIRNILESQIQITSKRHKEGIAIFDSAGGYAPTLGIIG
                     TVMGLVHILGNLSSNPDALGPQISVAFLATLYGVGSANLIWLPIASKLKAINQQEISE
                     RLLVIEAILSILEGDNSNLMTEKLKIFLNSKELIELEEIGGRAE"
     gene            complement(8964..9054)
                     /locus_tag="KPD58_000107"
     tRNA            complement(8964..9054)
                     /locus_tag="KPD58_000107"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9018..9020),aa:Ser,seq:gct)
     gene            9313..10077
                     /locus_tag="KPD58_000108"
     CDS             9313..10077
                     /locus_tag="KPD58_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBG0263903.1"
                     /translation="MLPQERYEKILSILKSDNIIKINDIVQMFDISIETARRDLAHME
                     SIGLLKRVYGGAIPVIASSVELNYVKRKKINSNDKKQIAIKCSEFINTNDTIFMDTGT
                     TVLEVSKNLKDKKNLTVITNSILVANELMNTNIKVYVLGGYLRETEGSFSGPLTLSAI
                     EQFNVDKAILGAGGITLENGISDYHLEEALVRKKMIERSRKSIVVVEPPKFGINSFAS
                     VAPIEHIDTIITSASIDHNIIIEFSQKGIPLVIADN"
     gene            complement(10358..11473)
                     /locus_tag="KPD58_000109"
     CDS             complement(10358..11473)
                     /locus_tag="KPD58_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HBG0263904.1"
                     /translation="MPFDKIILYIMAVGVLLGALDRIIGNKFGLGEQFEEGFNSMGPL
                     ALGMVGIVCLAPVISDVLGPIIIPIFNLCGADPSMFATILANDMGGYPLAMELAHNKE
                     AGMLAGLVVSSMLGCTLVFSIPVGLGLIEYEDRPFFAKGLLIGLITIPFGGIIGGLIA
                     GFNLSMVIINMIPVIILAVILAVGLKFNSTLMINGSLIFGKFISILITIGLGAAAFQE
                     ITGIVLIKGMTPIREGIQVIGSIAIVLLGTFPILHLLVKVLNKPLTIVGGKLGMDATS
                     AAGLVFTLANSIPVYKMMKDMSPKGKVVNTAWLVCATAALGDHLGFTAGVVPEMITPV
                     VIGKLVGGVLAIALAILMTKDLTLEEEQSIKMKKDAV"
     gene            complement(11514..13040)
                     /gene="glpK"
                     /locus_tag="KPD58_000110"
     CDS             complement(11514..13040)
                     /gene="glpK"
                     /locus_tag="KPD58_000110"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBG0263905.1"
                     /translation="MKYVLAIDQGTTGTRAVLINEKSEFVFSDYMEHTQIYPQSGYVE
                     HDPIEIWNNTKTVSNNVLQQAFENGINESDIKGIGIDNQGETVMLWDKNTGKPLYNAI
                     VWQCRRTYDYVENLKNIPGLENKIIDKTGLIIDPYFSGTKIKWVIDNVKGVKEKIKNG
                     EVLAGTLDSWLIWKMTGGKSFLTDVSTAARTMLFNIRDMDWDRELLDIIGIPRSILAD
                     IMPTSGDFGTTDKEAFCGVSIKITASMVDQPAALFGNGCFEPGMVKNTYGTGCFLYMN
                     IGEELNIAGNGILTTVAWKIGDKVTYAYDGGVYIAGAAIQWLRDGIGVIKNYSETDDM
                     ANSISSTGGVYFVPAFAGIAAPYWDQYARGTMVGITGSTKKEHIVRATLESVALQVKD
                     VVDSMNLASGKKIKMLRVDGGITKNNFTMQFQSDILGIPIEIAENPETTVLGVGYMAG
                     LSCGVWNSIDEIRSEFTISKKYIPNMTNQKREEILLGWNQAVKRAMNWENDTKKVCAN
                     "
     gene            13344..14441
                     /locus_tag="KPD58_000111"
     CDS             13344..14441
                     /locus_tag="KPD58_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBG0263906.1"
                     /translation="MKKTYLFSPGPVMVTDKVRNSLLHYDICHRGNEFMNLFKDTQQK
                     INKLYNATSNYYSLIVSGSGTSVNECVLSSIFDKEDAVLLVSNGVFGERLEEILSTYN
                     VKTYKPNFEWAEYPDLDILEKYIIENSDIKVIAMVFHETSSGMINPVPELGKLAKKYN
                     KIFFVDAISASAGEYIDVEEFNIDIITGVGGKALGAFPGSAYLCAKESILQNIKENQC
                     KNVYLNLYKHYKVAKSSSQTPNTPNVTLIFALNEALTEFLEDDSKIERYKECSAILRN
                     GMEELGLTFLLPDKYMSNTVTSVFLPKEINVNEFILGLERESGYVVYPGKGKFLDANI
                     FQVANMGEIYPDDCYKFLDILKSKLEIKKVI"
     gene            complement(14932..15444)
                     /locus_tag="KPD58_000112"
     CDS             complement(14932..15444)
                     /locus_tag="KPD58_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0263907.1"
                     /translation="MKVKKFKSGIVMALTVMAIGASLTNVSAMELNKSKEFETKYNEM
                     KRDFQEEYGKEDKFNITLDDEFTKEEVNGAKGIIKTTNKMNNEVVVLNYFDEMKSGIE
                     FELKPKAEKNAILRDEFQQGYGKEDKFKVVLDDEFTKEEVNGAKGIIKTTDKETGEVI
                     VYNYFDELEK"
     gene            15997..16878
                     /locus_tag="KPD58_000113"
     CDS             15997..16878
                     /locus_tag="KPD58_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HBG0263908.1"
                     /translation="MKKRLIIMILSVVLVLSSILTIFAYSNIKYNNNKLIYSNMIDKK
                     TQNSVKEILKENKINEKDIDTFIKAVNNYNKLQVKILQNNINISKSGYSSINAKQVPY
                     NLEKLQDNWVKKFPDYMDVNCRITAFRLFKDFINSNKKFTGDSIDLNVDLDTIMNNKD
                     AKFSTKDVEKFINFFSAIPAKDTDDTIKIAEQIKNEWKKRKISFKNNKNISIINGFLR
                     YPETKNVFIGHTGICIKTKNGILFLEKYGVTSPYQVTKFKNKEDVKNYMFNRLKMSEG
                     EIELPDPIIMENDKLMK"
     gene            complement(17191..17970)
                     /gene="hisJ"
                     /locus_tag="KPD58_000114"
     CDS             complement(17191..17970)
                     /gene="hisJ"
                     /locus_tag="KPD58_000114"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ"
                     /protein_id="HBG0263909.1"
                     /translation="MKDGHIHSPYCPHGSKDDFEKYVQRAMDVGITEMTFTEHFPYAN
                     GFRDPAPENDSCMSIEDLPKYFNDVKKLKEKYEGKIKINVGSEVDFIEGYEEGIKANL
                     NKYGEELEDSLLSVHIIKIDDDYYCVDYSVEEFQNLIDKLGSIEAVYNKYYETLIKAV
                     NSDLGIHKPKRIGHLNLVRKFNQVFPYDYKGNTILEELIKLIKEKGYELDYNVSGNRK
                     EYCKEPYIDGYLLELVKKYDIPLVLGSDSHCAEEINKYDII"
     gene            complement(18516..18866)
                     /locus_tag="KPD58_000115"
     CDS             complement(18516..18866)
                     /locus_tag="KPD58_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter"
                     /protein_id="HBG0263910.1"
                     /translation="MIFDKEIVRYHHGHHDHDHCHEHTHGDICHEHPHDHAHDHDHEH
                     SHEESSESKDEKTLKILLVHWINHNETHEEGFREWVEKARAIGKEETAKSIEKAIEYM
                     EEANKMLLEAKKHM"
     gene            complement(18882..19298)
                     /locus_tag="KPD58_000116"
     CDS             complement(18882..19298)
                     /locus_tag="KPD58_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HBG0263911.1"
                     /translation="MIIAVIDGMGGGIGAQIVSSLREELPTYVEIYALGTNSIATSSM
                     MKAHANKGATGENAIVVSAKKANIIVAPISVIIPNSMMGEVTCNISEAIADSEALKIL
                     LPIMPENVELVGLEGKPLALLVKDSVNLIKKEFNIK"
     gene            complement(19317..19508)
                     /locus_tag="KPD58_000117"
     CDS             complement(19317..19508)
                     /locus_tag="KPD58_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HBG0263912.1"
                     /translation="MCESSAFICKSNNELEKVMENVVNIDPCDGKIYLTDLLGEQKII
                     DGIIKEIRLMDHKIIIQEN"
     gene            complement(19659..20474)
                     /locus_tag="KPD58_000118"
     CDS             complement(19659..20474)
                     /locus_tag="KPD58_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HBG0263913.1"
                     /translation="MNQWRVIHNKSYEGAMNMAIDEAIFTAYKKGHNKPTLRFYTWEP
                     ACLSIGYFQKLEDEIDLDKCRCMNIDYTRRITGGRAVLHDNELTYSIIIGEDNPLIDK
                     SINLSYRYISEGLVKGLNLSGIETDNLNRGERISRENLSAACFNAHASYEVTINNKKV
                     IGSAQSRKDGVLLQHGSIILDFDVEKLFKLIKTKTPELKERAMKFTAKKASGIENEIG
                     RKIDIDILQKNIVKGLAEQFNVEFVEGDLTDYEKQLVKELYEKYKNEEYNKKR"
     gene            complement(20521..21459)
                     /locus_tag="KPD58_000119"
     CDS             complement(20521..21459)
                     /locus_tag="KPD58_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG0263914.1"
                     /translation="MIRLSVGTAIELGILNKKSDIPPTTAYIMIGEKCINKCSFCSQS
                     IESSTRKDKLSRVIWPEFSKEEILDALKAYKGKNIKRICIQSMASEEAHNSVLDFINY
                     ISGKIDMPISLSAKLENDEQIKKFFSAGVNKIGIAIDAANKELYEKIKGNNYDEKLKF
                     ITEMSKSYPNKISTHIIVGMGESHEDIYNLYTYLKENDVTISLFAFTPVRGTKMEKIS
                     QPSIESYRRVQLMSYMINKGYPKEYFKFKNGYLNSIKLDNDILKDINKGYPFEIRGCK
                     DCNRPYYNERPGSTIYNYSRPLNQSEIDLAIREINL"
     gene            complement(21456..22328)
                     /locus_tag="KPD58_000120"
     CDS             complement(21456..22328)
                     /locus_tag="KPD58_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG0263915.1"
                     /translation="MNKNLSEKMKIAFDIKLKNFGNKIEFAYPNQTLALSTTSNQCSL
                     KCAHCNGHYLNNMVPIEEYEEKVQSRNITSFLLSGGCSYEGDVPINTHINTIKNLKEQ
                     GYRLNAHLGLMDKDSIVELCKYLDIVSFDLVFDDETIREVYKMKKSKEDYIEVYNTIQ
                     EHTEVAPHICIGLKGGQIKGEYEIIEYLQKNPPNKLTFIVLIPTKGTEYENVEPPELE
                     GVADILCEARINLPDTEINLGCMRPRGVYRKELDQLSIMCGVNRIVLPSRSAKNKAIE
                     MNMTINECKECCVL"
     gene            complement(22353..24281)
                     /locus_tag="KPD58_000121"
     CDS             complement(22353..24281)
                     /locus_tag="KPD58_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4445 domain-containing protein"
                     /protein_id="HBG0263916.1"
                     /translation="MIKVSFTPNNKEVYCNEGDILLEVARNADIFIDAPCNGNVSCGK
                     CKVKLLNGKVDTEKTRHITDDEWEQGYILACCTKVISDIEIEVPSKVSSSMHGMKIEG
                     SNKKEDREIFERAKKIIEEHNLQFKTNIKKKYIEMEEPNLDDNISDVDRLERYVRNNL
                     GYNEIDFRLDILRKMPTVFRKSDFKVTITYVQKQKKLTIINIEQGNKENSLYGVAIDI
                     GTTSVVVCLVDLYSKEVVDKASSGNAQIKYGADVINRIIYSTKKNGLETLHKAIVEET
                     INPLLKTIYERNGIDKEDVVTLVAAGNTTMTSLFLGVYTDFLRQEPYIPPFLKSPKLM
                     GENVGLFVNDSAYVYLAPSVASYVGGDITAGVLSAGIWSSEENVLFIDLGTNGEIVFG
                     NKDYMMSCACSAGPAFEGGGISCGMRASAGAIEKVIIDKDTLEPTLKIIDECAPVGIC
                     GSGIIDLICQMITKGVIDRRGKIYRDLDNKRVRFNEHEIGEYVLAFKEEFDLENDIVV
                     NEVDIDNFIRAKGAIYSGAYTLVDSLGMDFSILDRVYIAGGIGNNLDIENSIIIGLLP
                     DIDREKFTYIGNSSLVGSYLALISKDAKNKLEEIGNQITYVELSVYPSYMDEFISACF
                     LPHTNIEQFPTAKKLLEE"
     gene            complement(24393..24770)
                     /gene="gcvH"
                     /locus_tag="KPD58_000122"
     CDS             complement(24393..24770)
                     /gene="gcvH"
                     /locus_tag="KPD58_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HBG0263917.1"
                     /translation="MKLLPELKYSKDHEWVKVIDGDVVYIGITDYAQDQLGEILFVET
                     PEVEDTVTKGVDFGVVESSKVASDLISPVNGEVLEVNEKLEDEPECINEDPYENWILK
                     VKLADVAELDTLLSDKEYEAGLE"
     gene            complement(24857..26983)
                     /gene="cdhC"
                     /locus_tag="KPD58_000123"
     CDS             complement(24857..26983)
                     /gene="cdhC"
                     /locus_tag="KPD58_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/CO-methylating acetyl-CoA
                     synthase complex subunit beta"
                     /protein_id="HBG0263918.1"
                     /translation="MNLYNIIFTGSEQALGAAQAMLAEAIEKNGKEHKVAFPDTAYSL
                     PCIYAATGQKMNTLGDLEGALEVVKSLINRTHLLEHAFNAGLATALAAEVIEALKYST
                     MDAPYSEPCAGHITDPIIRSLGVPLVTGDIPGVAVVLGECPDAESAAKVIKDYQSKGL
                     LTFLVGKVIDQAIEAGVKMGLELRVIPLGYDVTSVIHVVSVAVRAALIFGGLTPGDLN
                     GLLEYTANRVPAFVNAFGPLSELVVSAGAGAIALGFPVITDQTVLEVPMNLLTQKDYD
                     KIVATSLEARGIKIKVTEIPIPVSFAAAFEGERIRKSDMFAEFGGNRTEAWELVVKKE
                     ATEVEDHKIEIIGPNIDEVDADGVLRLPLAVIVKIAGKNMQEDFEPVLERRFHYFLNY
                     IEGVMHVGQRDMAWVRISKDAFDKGFRLEHIGEVLYAKMLDEFESVVDKCEITIITDA
                     EKVSELKGEAIAKYNARDERLASLVDESVDTFYSCNLCQSFAPAHVCVVTPERLGLCG
                     AVSWLDAKATKELDPTGPCQPIEKGECLDDRTGVWNSVNETVNQISQGAVESVTLYSI
                     LEDPMTSCGCFECICGIMPEANGFVVVNREFASVTPVGMTFGELASMTGGGVQTPGFM
                     GHGRHFISSKKFAYAEGGPERIVWMPKELKDYVADKLNATVKEMTGIENFCDMVCDET
                     IADDSEGVLAFLEEKGHPALAMESVM"
     gene            complement(27040..27846)
                     /locus_tag="KPD58_000124"
     CDS             complement(27040..27846)
                     /locus_tag="KPD58_000124"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBG0263919.1"
                     /translation="MEKFMIIGERIHCISPSIRKALAERDPAPILKRAKEQLEAGAHY
                     IDFNIGPAERDGEEIMTWGIKLLQSEFNNVPIALDTANKKAIEAGLKVYDRTNAKPII
                     NSADAGSRFDLIDIAAEYEAMVIGLCAKEGIPRDNDERMAYCQEILEKGLMLGMEPTD
                     ILFDPLCLVIKGMQEKQVEVLEAIKMMTEMGLLTTGGLSNVSNGCPKHVRPVLDSAFL
                     AMAMANGFSSAIMNPCDPELMKTVKSCDIINGASLYADSFLELNEGGFAF"
     gene            complement(27896..29263)
                     /locus_tag="KPD58_000125"
     CDS             complement(27896..29263)
                     /locus_tag="KPD58_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HBG0263920.1"
                     /translation="MALKALDIFKLTPKKNCKDCGFPTCMAFSMKVASGAVEVGKCPH
                     MSDDAIAKLSEATAPLMKALKVGAGASEYELGGETVLFRHEKTLVSRNRYAVSFCTCM
                     SDEAVDAKIANMKKVDYVRIGEQMKVEMAVLEYCGDKDAYLKLIDKIKGSGLEVAYIL
                     ACDDAQVVKEAVEVLKDARPMVYGATKENYKDMIEVVKGASLPLGVKAGSLEELYETV
                     ELIQAAGYKELVLDVTGENIKDTYTNAIQVRRTALKEQDRTFGYPSIVFANRLSNSNP
                     MMEVALSSIFTIKYGSIIVIDDISYAKALPLFALRQNIYTDPQRPMRVEPKIYPINNP
                     DENSPVLVTVDFALTYFIVAGDIERSKVPVWLVIPDAGGYSVLTSWAAGKFGGNSISA
                     FIKESKVEEVTNCKDLIIPGKVAVLKGDIEDNLPGWNVVIGPEESMELPKFLKGYQEK
                     ACQTN"
     gene            complement(29294..30238)
                     /locus_tag="KPD58_000126"
     CDS             complement(29294..30238)
                     /locus_tag="KPD58_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HBG0263921.1"
                     /translation="MAFKMSTQKYSGKISEVEVGIGEKAIKLGGENVLPFYSFDGEVG
                     NSPKIGIQISDVYPESWTDSYKELYKDVANCPVEWAKYVEANTQADFICLKFDGSDPN
                     GLDKSVDECADVAKAVIEAIKLPLVVAGSGNHEKDGKLFEKLAQTLDGHNCLFMSAVE
                     DNYKGVGASAGMAYAHKVGAESSVDINLAKQLNVLLTQLGVKGENIVMNVGCSAVGYG
                     YEYVASTMDRIRLAAFGQNDKTLQMPIITPVAFEVGHVKEAIAPIEDEPDWGCPEERT
                     IAMEVSTAASVLVGGSNAVILRHPKSIETIKELVNALA"
     gene            complement(30258..31028)
                     /locus_tag="KPD58_000127"
     CDS             complement(30258..31028)
                     /locus_tag="KPD58_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0263922.1"
                     /translation="MGYNIAVAGKGGTGKTSLTGLLIDYLVKDKKGPVLVVDADANAN
                     INEVLGIEVEATIGEIREEVNQREKLGNAFPGGMTKAQYLQFRLNSIIEEGEGYDLLV
                     MGRSEGEGCYCFVNGILREQVNKISGHYKYLVMDNEAGMEHLSRKVTRHVDTLLLVSD
                     CSRRSIQAVARIRDLAEELKLSVGRILLIVNKVPNGVMNDGVKEEIEKHNLELIGVVP
                     MDELIYEYDSTGIPLVNLPEDSKSKVAMKEIFAKLELK"
     gene            complement(31084..32469)
                     /gene="lpdA"
                     /locus_tag="KPD58_000128"
     CDS             complement(31084..32469)
                     /gene="lpdA"
                     /locus_tag="KPD58_000128"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBG0263923.1"
                     /translation="MKIVVVGGGPGGYVAAIKASMLGADVTVVEKRRVGGTCLNAGCI
                     PTKALLASSGVLNTVKEAKDFGIEIDGTVKPNFTAIMERKNKVVNQLISGIEFLFEKR
                     GVNLVNGFGKLIDKNTIEVTKDDGTVETIKADKIILANGSVPVVPRMFPYDGKVVITS
                     DEVLGLEEIPESMLIVGGGVIGCEIGQFFRALGTEVTIVEMVDQILLNEDKDVAKQLL
                     RQFKKDKIKVITGIGVQTCEVVDGKAVATLSNGKVIEAQYALVCVGRRPNLDNSGVED
                     IGIEMERGKVVVNEHLETNVEGIYAIGDIIDTPFLAHVASKEGIVAVENALGKTKVVD
                     YRAIPRCVYTEPEVAGVGKTEKQLEAEGVEYNVGQFDFRGLGKAQAIGHFQGFVKVIA
                     DKETDKIIGAAVVGPHATDLLTELSLAVHLGLTVEQVGDAIHPHPSLSEGLMEALHDV
                     HGECVHSVPKL"
     gene            complement(32513..33394)
                     /locus_tag="KPD58_000129"
     CDS             complement(32513..33394)
                     /locus_tag="KPD58_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HBG0263924.1"
                     /translation="MSLLRETLESGKFAVTTEMAPPKGTDLSHLIECAKPLVGRVHAA
                     NVTDFQSAVMRATSLATCKLLKDAGLEPVIQITGRDRNRIAIQGEMLSAGVFGINNLL
                     ALTGDHTSVGDHPQAKGVFDLDSVGILQTAETLMAGTDMAGNKLKGSPDFYLGASVTP
                     EYSPIEVQLLKMKKKIKAGAKFFQTQALYDINTMRKFRELTRDMDCKVLAGIVPLKSP
                     GMAKFMTANVPGIFVPDEQIERLRAAGKENWVSEGIKMAGELIKQLKEEDLCDGVHIM
                     AIGAEENIPAILDAAGL"
     gene            complement(33430..34104)
                     /locus_tag="KPD58_000130"
     CDS             complement(33430..34104)
                     /locus_tag="KPD58_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HBG0263925.1"
                     /translation="MIISENKPLEEVLGYLKDFDKLVLVGCNQCAATCKSGGEEEVLK
                     MKETLEGEGKKILGYVMLDPACNLLKSKKDLKALKEETKEADAVLSLACGDGTQTIVK
                     NLKDKPVYPANNTLFIGEVQRVGEYEEACKACGDCELGWTGGICPVTMCAKGLMNGAC
                     GGAKNGKCEVNSENDCAWIKIYERLEAIGQLDNLAEIRPPKDYSKQNNPRSLSAKKKK
                     EAAANS"
     gene            complement(34139..35011)
                     /locus_tag="KPD58_000131"
     CDS             complement(34139..35011)
                     /locus_tag="KPD58_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HBG0263926.1"
                     /translation="MEGMSTKGQIIKGKPVADKISEELIKEVDLLVKEGINPKLTIVR
                     VGARSDDLSYERGALKRCQNIGITTEVLELAEDITQEEYIDVLKRVNDDKNVNGILCF
                     RPLPKHLNEEVIKYVIAPEKDVDCFSPINSAKVMEGDKSGFPPCTPTAVVEILKHYNV
                     DLKGSKVTVLGRSMVVGKPVSMLLLSEHATVTICHSKTKNLSGVAAEADVLIAAIGRA
                     KMVDESFVKDGAVVIDVGINVDEEGNLCGDVDTNAVLDKVSMITPVPAGVGSVTTSIL
                     AKHVVKACKLQNNK"
     gene            complement(35103..35735)
                     /locus_tag="KPD58_000132"
     CDS             complement(35103..35735)
                     /locus_tag="KPD58_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HBG0263927.1"
                     /translation="MKIADKTCVDFVEVLSSKEAVPGGGGAAALVGAIGMALGSMVCN
                     LTIGKKKYAEYEESVKEILSKAGKLEKDLLKMIDDDAECFLPLSKAYGLPKETEEEKR
                     IKAETMEKALKVACEVPLNIVRVCYEAIKLHEDLVDKGSRLAISDVGVGVQCLRAAIL
                     GGQLNVVININSIQDKEYVNKVKTEVDKLVEDGVKICDEVYAKVEKALGK"
     gene            complement(35840..37516)
                     /locus_tag="KPD58_000133"
     CDS             complement(35840..37516)
                     /locus_tag="KPD58_000133"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HBG0263928.1"
                     /translation="MGFKSDIEIAQEAKPQDIREVAKKLGLGEDDVELYGKYKAKVDY
                     NLLKRETGKKAKLILTTAINPTPAGEGKTTTTIGVADAFAKLDKNVLVALREPSLGPV
                     FGVKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAANNLLAAMLDNHIHQGNALRIDPK
                     KITWRRCVDMNDRQLRNIVDGMGKKGDGAVRQDGFDITVASEIMAAFCLASDISDLKE
                     RLGNIIVGYSYEGEPVTARQLKANGAMAALLKDALKPNLVQTLEGTPSFVHGGPFANI
                     AHGCNSVIATRMAMHFADYVITEAGFGADLGAEKFLDIKCRMANLKPDAVIIVATVRA
                     LKYNGGVAKADLNNENLEALEKGLPNLLKHVENITQVYGLPAVVAINRFPLDTEAELK
                     LVEDKCKELGVNVALSEVWAKGGEGGIAVAKEVLRLLDEEENNFRFCYEDDLSIKDKI
                     NAIATKIYGADGVDYTPEADKEIANLEKLGFTKVPVCMAKTQYSLTDDQTKLGRPTGF
                     RITVRQATISAGAGFIVALTGEIMKMPGLPKVPAAEKIDVDENGVIAGLF"
     gene            complement(37765..38544)
                     /locus_tag="KPD58_000134"
     CDS             complement(37765..38544)
                     /locus_tag="KPD58_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0263929.1"
                     /translation="MKIAITGKGGVGKTTFSSMLSRMFAEDGYRVVAVDADPDANLAL
                     ALGFPKEVYESIVPISEMKKLVSDRTAASVGSFGKMFKMNPKVDDIPENFCKEYNGVR
                     LLTLGTVDSGGTGCVCPEHVLLKRLCSHLILQNKDVVVMDMEAGIEHLGRGTAQGVDA
                     FIVVVEPGERSLQTYRKVKKLGHDIGVNKVFVVGNKIRNKEDEEFIIQNLEDGESLGF
                     IYYNQDVIDSDRANQSPYDSSETTKEQIKAIKDKLMSLKDK"
     gene            complement(38854..40773)
                     /gene="cooS"
                     /locus_tag="KPD58_000135"
     CDS             complement(38854..40773)
                     /gene="cooS"
                     /locus_tag="KPD58_000135"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HBG0263930.1"
                     /translation="MDEKMLTIDLNSQKMIAKAREEGVETMYDRKEGFKAQCGFGLQG
                     VCCRICGMGPCRISPKTPRGLCGADEHTIVGRNFARMVAGGTAAHSDHARDIAHTLAL
                     ADPNGNYKIRDEAKLITLAKEWDVETEGRDIYDVAHEVAEIALMEFGKPFGTLRFIKN
                     APEPRQKIWKEYAIEPRAIDREIATIMHSTHIGCTGDIDSLIHMSLRTSMADGWGGSM
                     IGTRLSDILFGTPVPRRTEANLAVLEENKVNIILHGHEPALSEMIVLASEEPDLVALA
                     KEVGADGINLAGMCCTGNEITMRHGVKIAGDFHQQELAIITGAVEAVIVDVQCIFPAL
                     ARVADCYHTKFVTTSPKAKITGSTYIEFREEQALDDAKAIVKEAILNFKNRDKSKVLI
                     PELKSGATVGYSVEAVVNQLDRVVNSHIDPAGTVKPLTDCLKAGVLRGAAGVVGCNNA
                     KGVSNEAHVTIMKELIKNDIIVVTTGCGASAAAKFGLMESDAAEKYAGKGLATVCKLV
                     GIPPVLHMGSCVDISRILDLVGAAANYLDMDMCDLPVVGIAPEWMSEKAVAIGCYVVA
                     SGIDTYLGIMPPIAGSSRAVDILTSELKDKVGATFTVNTNPKELAATIIEDIEKKRVH
                     FEALVEEKMTEKAEA"
     gene            complement(41380..42291)
                     /locus_tag="KPD58_000136"
     CDS             complement(41380..42291)
                     /locus_tag="KPD58_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HBG0263931.1"
                     /translation="MIKKLDDILEQLKGGEKIVLSVAAAHDKEVLMAIKDAVERNIIT
                     PILVGNEGKIREISKEIGFDLSGIKIVDKDDIKECAEIAVKLVSSKEADFVMKGLLDT
                     SVILKEVLNKDYGLRTDSLLSHVMVYELEKYHKLLITTDGGMNIAPDYEQKAKILKNS
                     IKAAKALGMETVKVACLAAKEKVNPKMQATVDADMLAKACKEGEFGENVIVEGPLAFD
                     LAVSKEASEIKGFKSEVSGDVDIILVPTIEVGNGIGKAFTYMADSKSAGIIMGAKAPI
                     VLVSRADSHESKLYSIAYGAIVAKNMK"
     gene            complement(42407..42985)
                     /locus_tag="KPD58_000137"
     CDS             complement(42407..42985)
                     /locus_tag="KPD58_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBG0263932.1"
                     /translation="MNINEFLTHINQRKPVSSKSEIHEFMVKLSNEAMKITSKLNSSY
                     HEPEEVRSLFSELIGKKVDESFFMFPPFYTDCGKNITIGKNVFINSSCHFQDQGGIDI
                     GDNTQIGHNVVLATLNHGIAPEKRGTTYPSPITIGKNVWIGANVTIVPGVTIGDNVII
                     AAGAVVTKNVAENTIVGGVPAKLLRVIDEIPE"
     gene            43211..44134
                     /locus_tag="KPD58_000138"
     CDS             43211..44134
                     /locus_tag="KPD58_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG0263933.1"
                     /translation="MEIRVLRYFLAIAREESISGAATFLHITQPTLSRQIMELEEELG
                     VKLLVRGNRKITLTNEGRLLHKRAEEIISLVDKVELEFDNPNEIISGDIYIGSGETEA
                     MRLIARTAHHFHQKYPNVRYHLFSGNADDVTEKLDKGLLDFGILIEPANIQKYDYAQI
                     PATDVWGLLMRKDSALASQSTINPKDLCNIPLIISRQTLVDKAISKWLQDSNEKLNII
                     ATYNLVYNASLLVEEGLGYALCLDRLVNTTENSNLCFRPLSPPLEAKLNIIWKKHQIF
                     SKAAEKFLLQIQKDFSSIMLDTSKLDTPKEK"
     gene            complement(44306..45448)
                     /locus_tag="KPD58_000139"
     CDS             complement(44306..45448)
                     /locus_tag="KPD58_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG0263934.1"
                     /translation="MDFKIEKYLLKKTEELAFITIKHGGEFKLKSYEVPKGGLDVPIK
                     NEVLVKGIKEKTAQDKLNSMSIADAMIYIIGIDSKFKNNAEYEKFLNALSKDIDLDLK
                     SYMGYMSRKYFEIGEHTDSLIYIKAFITMYPDDLDAMYNYAIVCQEIAKQYQKDMDDK
                     AMNAFLLEAMAKLEKVIDVDENFALGYYHLGYHYYNQGQYLKTKLTWEEALRLGLDAD
                     LVAEVQENLGKMDFKVQYEEGYTLVFQGKFKEGLEKLLPLEEEHMDWWNLLFMIALGY
                     KGMGEIEQAKMYLEKILIIKPNQVDTIVELGLCEAYKNNLDKAIEYFEQAAKIKEDPE
                     ILCNLGMAYLNNGDIDDATYYIERAYELNPQDEITIACLRELGINK"
     gene            complement(45466..47220)
                     /locus_tag="KPD58_000140"
     CDS             complement(45466..47220)
                     /locus_tag="KPD58_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HBG0263935.1"
                     /translation="MKILLTTLNSKFIHTNLAIRYLKEFVRDLIEVNMKEYTINNDLD
                     YILKDIYKNEYDIILFSTYIWNVGDIVKLCDNLKKIRPNTKIALGGPEVSYDSYEAMK
                     KYDFVDYILYGEGELIFRDLVLHLQGKMEINDVNGLVYRQGNEIIVNKPMELLQNLDE
                     IPSPYENLNPKEYENRIVYYESSRGCPFNCQYCLSSTIPGLRYFSLDRIKSDLKALID
                     ARVSQIKFIDRTFNANRKVAMEIMDFLMKNDNGYTTYHFEVTAYLIDDKMLEFLADCK
                     EGLFQFEIGVQSTNEKTLDAVGRRDDFKKLSHVVQTVASYRNIHQHLDLIAGLPYEDY
                     KSFENSFNDVFNLGIEHLQLGFLKMIKGTGMRKVADEHGFKYKDYAPYEFLYNNYISY
                     EETLKLKDIEDILERYYNSKNFVLSMRYIIGRFYKKSPFKFFEVFAKYFDENGYFDLA
                     QGKNQLYKILMDFYNEKINIDNDVFNDILKYDYISLGKTSNIPQFFNKLDVDDFKNRC
                     HVFLQDNDNLSTYLPSFVDKPAKHIIKYVHFEPFRFNVVDLKNDINTEIREVENVVLF
                     EYDDKKIFEKSKTHKVEI"
     gene            47356..47562
                     /locus_tag="KPD58_000141"
     CDS             47356..47562
                     /locus_tag="KPD58_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0263936.1"
                     /translation="MELIEKVSINSLSKRDLLLIIKSLEYTNENTQISDFIDLRNNIV
                     KELCFLTDTKEKDFLDYLEKNSTL"
     gene            complement(47567..48490)
                     /locus_tag="KPD58_000142"
     CDS             complement(47567..48490)
                     /locus_tag="KPD58_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0263937.1"
                     /translation="MNDELLKKVYTSSFSSASRALKDLTIINTKSLIDDKTQRCVYID
                     ENRLRDELIYYRFYGEKIGNINFLNILLPLILSNTNIQKSEDEVIKLIKKYVIYFKKE
                     ELLFDYLLGSVVYNSIMHNLINNSKIEYVELLQSIKDKIIGFSIELDKSDVVKFQMAR
                     IKAIQLIDKYIDLKSEDYDEESILLNVLNVLYDVYMEDRIVENEGINSIKKSILSILG
                     EDSKLNEDNIDFIFSMSEYVVKLRKYKIGVKAYNKSIDPRSLIRLEEGNTIIDPIFNQ
                     ITVMSKTFNDNILSIKINSKSGIYILKFKKV"
     gene            complement(48745..50445)
                     /locus_tag="KPD58_000143"
     CDS             complement(48745..50445)
                     /locus_tag="KPD58_000143"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBG0263938.1"
                     /translation="MQDFKVAISNCLKEKIEDLSKEEIEALIEVPPNKDMGDYAFPCF
                     KLAKVFRKAPNMIASELAESIEPSGEITKVIQLGGYVNFFVNKSQLAETVIKKVLDEK
                     ENYGHSDFGKDKTVIVEYSSPNIAKPFHIGHIRTTVIGNALYKIYDSQGYKTIRINHL
                     GDYGTQFGKLIVAFKKWGEKEVVESNPIPELLKLYVRFHDEAEQHPEMEDEARAWFNK
                     LENGDEEAQELWQWFRNESLKEFNRVYKLLDIEFDSLAGESFYSDKMNRVIELLEEKN
                     LLKESKGARIVDLEEYKMPPALITKNDGSTLYMTRDLAAAIYRKETYDFDKCIYVVGS
                     QQNLHFQQWFKVIELMGYDWAKDLIHVGFGMVALEEGTMSTRKGRVVFLEDALNQAID
                     KTKEIILAKNPNAKNVDEISKQVGVGAVVFQELSNSRIKDYTFSWERTLSFDGETGPY
                     VQYTHARCCAVLRKAEVEVTSDIDYSLLADEDSAEVLRVIESFNKNILLALKKNEPHI
                     VTRFMLDLAQAFNKFYHDNPILVENLEIRKARLALVLATKQTLENSLKLLGMHAPERM
                     "
     gene            complement(50908..51321)
                     /locus_tag="KPD58_000144"
     CDS             complement(50908..51321)
                     /locus_tag="KPD58_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBG0263939.1"
                     /translation="MILVSACLIGINCKYSGDNNDNEKVKEYLKGKEFTLVCPEQLGG
                     LSTPRPPAEIVDGKVITKDNKDVTENFVRGANETLKIANLYVCSEAILKEGSPSCGCN
                     LIYDGSFSGKKIPGKGITARLLEEEGIKVKSEKDL"
     gene            complement(51387..53765)
                     /locus_tag="KPD58_000145"
     CDS             complement(51387..53765)
                     /locus_tag="KPD58_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBG0263940.1"
                     /translation="MNEKSLRVLEYNKIIDLLKKKASSSLGLKYIENLVPNTDFVEVK
                     SMLEETSEAQSIIIKRGSVGLEGIHDIEDKVKRAYIGASLDPGSLIMIADTLRVARRL
                     RNSLSSSDEEDFNYPIIQSLSNSLYVYKDIEDQIYNAIISEVEISDNASSTLRDIRRR
                     IAQKNQSIRSKLNSIISSTTYQKYLQDAIISLRGDRFVVPVKSEYRSQVAGIVHDQSS
                     SGATLFIEPMTIVEMNNELRQLKLGEQEEIERILSELSAMVGEVSEDLISNQEILGRL
                     DFAFSKGKLSIQMRGIEPTLNEDKYLNIKNGRHPLLDKKKVVANTIYLGRDFHTLVIT
                     GPNTGGKTVTIKTVGLFALMTQSGLHIPADYGSSMCVYDNVFADIGDEQSIEQSLSTF
                     SSHMTNIVSILQNVTADSLVIFDELGAGTDPVEGAALAIAVLEDINSVGAKCIATTHY
                     SELKNYALTKSGVENAAVEFDIETLSPTYKLLIGVPGKSNAFEISRKLGLSDYVISRA
                     KEYINTENIALEDVLQNVEKNRIKAVEDREEAERLKEEIEKLKVEYDEKLEKLVSQRD
                     KMIEKAKSEAFSIIRQAKEEVDIIIKELRSLEQERASKEKNRKIEELRKELTSSMGSL
                     QPTVKSMIVPKVSNKEIKDLKPGEEVKVITLNQNGSVVSVDKKRKEAVVQIGIMKMTL
                     PFKSLQKTRKDVSTNVTKSTRNIIRSKSGSVKNEVDLRGLNLEEAIMEVEKYLDDAYV
                     AGLESVTVIHGIGTGVLKAGLQDILRRNRHVKSQRGGQYGEGGAGVTIVKLK"
     gene            54084..55535
                     /locus_tag="KPD58_000146"
     CDS             54084..55535
                     /locus_tag="KPD58_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HBG0263941.1"
                     /translation="MGNVLEGLKPESVFKNFEKISQIPRGSGNEKGISDFLLSFGKNL
                     GLETIQDESLNIIIRKPATKGYENCPGVVLQGHMDMVCEKEKNVEHDFLKDPIKLRID
                     GDMIYATGTTLGADNGIAVAMGMAILEDNTLEHPALEVLVTVNEEDGMNGADALDPSL
                     IKGQYILNMDSEEEGYLLVSCAGGKTCVVSLPVEYKEVKGDKQGLLVEVTGLLGGHSG
                     MEIVLQRANANKAIARVLSVLNVDYELASVDGGTKHNAIPREAKCVIAVNKADVESAK
                     KQINDILTAFKHEFTTSDPGMTYSVAETSVDKVLTKDCKEKVVQMSCLTPHGVQSVSL
                     DIEGLVESSTNFAIIETKESTIEFLTSVRSSVMSIRDEIADRIRLLAQALGANYDLIA
                     QYPAWEFKKGSKLEKICSETYEKLTGKVPTVMALHAGLECGLLLDKLPHAEAISIGPD
                     MFDVHTPNEHVSIPSVANVWDYVIEILKSMNQY"
     gene            complement(55739..58252)
                     /locus_tag="KPD58_000147"
     CDS             complement(55739..58252)
                     /locus_tag="KPD58_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBG0263942.1"
                     /translation="MEDCVVNLKKLLLLIIVITSIFTSCIFSFGINAIGNDNRTIRVA
                     YPIQKGLTEIDEQGNYFGYTYEYLLEIAQYTGWNYEFVQVPGEINESITKLMKMLENG
                     EIDIMGGMLYNEPMQKIYNYAGNNYGVANTVLQALYENTDIDNIMSSQDVKKIRIAVI
                     ENSDTRLKELDEYCKMNLMSPELVYCKTNDEQLEALKSGKADVLLNVSMNPIEGVRTV
                     AKFSSKPFYFITPKGSTNLTQKLNSAINNIEQKDPYFSTTLYEKYFTPENNQMFLSDE
                     ETNYIKETEKLKVGVLLNKPPFQNIDKDTGELRGISIDLLNHICDKTGLEFELVPVKS
                     QEDLENMTKKREIDIVAGMIYDYEYSREHNIAMSRPFLSTQYTMMINNKLNENSLDGK
                     RVALAKGINYYGQFEENIVWYDNLEECIEAVNKGYADYTYGEGYSMQYYMNQPKYKNV
                     RLVPQTYKAKSICFGIIKKDKQELLNIFNKAIISISSEKMQSIVYQNTTYRQDFSLLY
                     IMQQNPIETIFIVASLLICIIGFMAFGLHTRTSMNEKISLELKKHTQLYEMTSEYFFE
                     YDYKQDKLIISMPEKGIETYYGLDKNDKESENGNIENERDKIRKEYNKKFFDVIKSQE
                     NGTTEMYSIMLDDTFHWIRVTSKIIYNDRKIPMYVIGKICNIDSEKEEKNRLLDKAQR
                     DSLTNIYNAAYIRKLTAQNLLNLSTEKNGALLIIDIDYFKSINDTYGHLLGDKVLIDV
                     AKVLDESFAKNDIVGRLGGDEFSVYINDIINKEELINKCNDVYNHIHLIKLPNGESLS
                     ISMGVVIAKPGQEYDELYQIADTALYDSKRQGRNRFKIV"
     gene            complement(58475..59296)
                     /locus_tag="KPD58_000148"
     CDS             complement(58475..59296)
                     /locus_tag="KPD58_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG0263943.1"
                     /translation="MIDINLEKCVGCGMCESDCLVNAIKVKDDKAKVKNILCINCGHC
                     MAICPTDAIEMQGFDKNEVIEYNRESFELEPEKLLNFIKFRRSIRQYKDIEVEEEKIK
                     NIVEAGRYTPTGGNRQPIRYILVKEKLKEVKELAIQGLYNLALDTDDNDPVRSMYKNT
                     FKKMYKRYKENGNDSLFYDAPLLMVVVGDMSLGGSAYVDGGLAASNMELMAYSQGLGI
                     CYNGFFVMASNVEPKIKELLGMSENEAVITSFILGYPDVKYKRTVNRNTAKFEVR"
     gene            complement(59815..60291)
                     /locus_tag="KPD58_000149"
     CDS             complement(59815..60291)
                     /locus_tag="KPD58_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HBG0263944.1"
                     /translation="MKLTTICYIEKDDKTLMLYRNKKKDDIHEGKYVGVGGKFEQGET
                     PEECVIREVKEETGLTLKSLSYKGLITFPKFKDEEDWYMFLYFSDEFEGELSEKDLND
                     CKEGNLIWVDNDKIFDLNMWEGDRLFLNWAKTGNIFSAKIVYDNGKLKDYNVSFLD"
     gene            complement(60326..60904)
                     /locus_tag="KPD58_000150"
     CDS             complement(60326..60904)
                     /locus_tag="KPD58_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBG0263945.1"
                     /translation="MREEALEILFKYLETDRMRKHCYAVEAVMRELAKRLEPEKEEEW
                     AVAGLLHDLDSDIVGRKPGEICEGHATTTVELLKKENFGDEEMYRAILGHHDGMGVTR
                     TSLMEKAIYAADPITGFITAIALVYPDKKLTSVKTKSVIKRMKETRFASNVNRDAMRS
                     IEDIGIPFDEFAELSLNAMKNISDVLESAENN"
     gene            complement(61008..63608)
                     /locus_tag="KPD58_000151"
     CDS             complement(61008..63608)
                     /locus_tag="KPD58_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBG0263946.1"
                     /translation="MKKIELLAPVGSFDSLKAAVQNGANAVYLGGKDFSARASANNFD
                     RNELIEAVKYSHIRGVQVFVTVNTLIKQYEIEDFVEYVKFLYDIDIDALIVQDIGMAR
                     LIKKLLPDFELHASTQMVAHSLEDVKYLQSVGFDRVVLARELNVDEIKWICENTTVDI
                     EVFVHGALCVCYSGQCLMSSMIGNRSGNRGRCAQPCRQKYELIDINTGEVVKSNGDYL
                     LSPRDLSTIEDLDKIIEAGVLSLKIEGRMKKPEYVATVVSGYREAINEFVDKKKISIS
                     EEIINNLYTIFNRKFTKGYILGEVGKDIMNSNRPNNQGLYIGKVLDYNRKNKRLRVML
                     ENTLKKGDGINLGGGTIGRIIKDGEITTIAHKGDLIELDFVGEAKRNQLIYKTSDSEL
                     MDRVQATFQQDKEFIKTNIRAKVSIKLGKKPELFISDFCGNKSVVEGDKIVEKAIKVA
                     LSKEKIETQIQKLGNTPYKLKEIEIDLDEGVSMPISVLNQMRRDCIELLDEERIKIKD
                     RKFKKNRLKYQPNRVNKQKKLDTAPKIRVKVKNLEQLEAVLNYNVDLIYYEDINTLSK
                     ALQMVSSISIDSNISIDSSISMDLDTSSSLGTPKELMGSNTNNIDTLSNTSKIDTLNK
                     NHYNTAVTDKKLIYSAPRIVRNKEYKQFEILDNIKGINKVQIGNWGSMEYFKKKDLNI
                     DCYLNAFNSESINHFKEEGASTVCLSQELNLNEIKQTLKYTDVEIEAVIYGYAPMMVT
                     EYCPMGVVVRDCKKDKRVAKCKESSYALRDFTDASYRITQDIFCRSTIYNSKVTCMLD
                     NLYELEEIGIDVFRIDFTVEDSEMVKKVIEAHIEVLSNNYKLGKKATDLYKKLDEIGT
                     VAGHYYKGVE"
BASE COUNT        19846 a        11967 c         7063 g        24990 t
ORIGIN      
        1 taaatagtat aaattatatt cttcaataaa caagttttca aatccaagat tcgctctcta
       61 tctttaaact ttattgtaaa cataattcca tcttttttaa ttatttctcc ttcatgaaat
      121 tttttatggt ataccttatc ccctacacca atactgtcaa tttctttttt cgttggagat
      181 tttatatcat taataaataa tgatttatca actttcttac catacttatt taaagtagag
      241 cttatacata acttttcttc agctcttgta atagcaacat acataagtct tctttcttct
      301 tctaattgtt cttctttttt ctcttcctta caaatatcat atgatttttc atgaggtatt
      361 gttccttcat ttacacctat aatataaaca tttctaaatt ctaatccttt tgcactatgc
      421 atagttgtaa atatgacacc ctcagtctgc ttattcttgt tattttccgt taactcagtc
      481 tttactctat caatatgctc taaaaactct gtaacagtat taaaattagt tgctgaactc
      541 tcaagttcat ttaatatctc tacaagtcca ttagtcttta tctttctgtt gctacaataa
      601 tctaaaacat atctatcata atcaagacta gttctaatat aagatattgc atttttagga
      661 tttaaagtat ttaaataact taaatctatt tccaaatcat ttattgtttt aacttgttta
      721 ggatgaagat tacacttatt tattaatgct gttataaagt ctttttcatc cttaaccata
      781 tttatactgt ctttggatat atatctaaaa ggcttgttta taattctcaa ccaatcttta
      841 tttgattttg gattaatacc tattcttaaa taggctagta tgtcttgact tgcccaatgg
      901 tcatatattg taattactga gtcttttact acaaatggaa tcctcatatc cataaataca
      961 tctactaatg ctcttgactg tatattagtt ctataaatta ctgcaaaatc tgaatactct
     1021 acataatctt tttgtatttc gtcaattatt tccctggcaa tatataaggc ttcttcctct
     1081 gaatcatgcg gagaaatata ggtaacactt cctccatccc cctgactaca cttaatgatt
     1141 ttttcatatc tatttttatt tttgtcgata agtctatttg cagtatgcac tatctcactt
     1201 ttagacctgt aatttatatc taatattatt tttttagtat tattaaagta ttgttcaaat
     1261 tcaagcaaaa aatctggtct agcacctcta aatccatata tactttggtc ttcatctcca
     1321 actgcaaata tattgtttaa tgggctacaa attaacttta aaacttcaaa ctgtacttta
     1381 tttatatctt gaaattcatc tatcaatata tatttataca catttctaac catttctagt
     1441 gcagacttat tgttcaaaag taaataataa gttcttataa gcatatcatc aaaatctatt
     1501 ttattaactt tagatttttg ttcttcgtat aagttgtata cttttaaaaa ctcatctttt
     1561 gttaatactt ctgaattaaa ttcattttta tccataagtt cattttttac ataggaaatt
     1621 tcatttataa cttgtcctat attctcatca tcatcagcat tctctatatt taaacttttt
     1681 aggattgcct tgattgtcat tcgctttgac ttttcatcaa atatactgtc taagttgtat
     1741 ctttcaaagt atcttagaat tttaaaaaat acagagtgaa aagtaccata ggttacctta
     1801 ttaagtctta tgtcatcact taaattcaaa gctctatttt tcatttctat tgaagatgcc
     1861 ttggtaaaac ttattgccaa gattcttgtt ggtgctatgt ttttgtttac aaccatattg
     1921 gctattctat atgtaattac tctggtctta cctgaaccag gccctgcaag tatcatgcaa
     1981 ggcccatcta tatgctcaac tgcttctaat tgattttcat ttaactttct tatatctata
     2041 tttgttgtga ttattagctc caccaccttg atttataatt atattatttt ctttttaatt
     2101 ataaactaaa agttcataaa aataaattta atttttcctc tattaaacca catcattcct
     2161 attttcatga catatcttga gttctttttt aattttgtcg cttatatctt caggtataaa
     2221 tgatggttta ctaatatagt agccttgcat ataatctact ccaagattaa ttagtgtttc
     2281 catttcacct tttgtttcta caccttctgc tatcacttta atatttcttt cgttagaata
     2341 tgagataaga ttttttaata ttttctgtct gttttcatct ttatcaatac ctcttacaat
     2401 agacatatca atctttacat agtctggcat tatatataat agtacagcat ctccgttgta
     2461 acctgtccca aaatcatcta aagcaatttc tatattccaa tggtctatat atgctttctt
     2521 tttctcaata aaatcttcat taatcttatc attctctgtt atttctagta ctactctatt
     2581 taaatattca ccatataaat cttcaatttc ctcaaaatct acttttgaca aattattatt
     2641 aggaatagaa tttaagaaca gtttgcaatc gccaaatttt tctttgtatt ttttaaatga
     2701 tttaagtgct ttaaaccatg taatacgctc aatttgatat aatttagact gtgaacgagc
     2761 taatctaatg acatctattg gcgaactaaa agtttttatt ttaggtctca taagtgcttc
     2821 atatgcaaat actttacctt tacaatctac aataggctga aatgcatact caactaattg
     2881 accatcaatt aacttgttta attcttctct attactcaat aagaatgaat ccttattata
     2941 tatattaata tcaaattcac taacttcacc cttgattgta tttttaattt gatacatagc
     3001 aaaatctgcg tatctcatta attcatcata gttggttgaa tcatcagggt accaggaaat
     3061 tcctgctgat gcccttattt taaattctgt tccatcatga agttttaata aggtattatt
     3121 catctgtttc ttaacagaat taataatatc tcttatacta tctttacttt catatccata
     3181 tataaataca taaaactcat cacctgaaag tctagatact ataccattcc atgatataaa
     3241 cttcttaagt atgtctgcag cacacctaat atattcatca ccagcatcat gtccatatgt
     3301 atcattaata tattttagat tatctaaatc ccacataata aatgctgcta tcttaagttc
     3361 atctttattc ttaaattttt cttgtatctg tgaatgaaaa gctctcctat ttaaaagatt
     3421 cgttaaaata tcaaaatctc tttcatattc tatcttcctt ttctcaataa attcttttgt
     3481 tacatcaaca acaacaccaa gaacctttag ctcatcttca aagatattca ttcttatcca
     3541 cttaagtttt ttatctttag attcaaactt atatatatat gtattttctg tttcataact
     3601 ttcttgcaaa tatttatcta gtccccttaa ttttttcagg aacttatttt tttccatata
     3661 tccatattct tcaaatttga tttcagtagt aaacacgcta aaaaattctt ttgtacaaaa
     3721 aacattctct gcgtttttgt ataactcaaa agctccaatt ggcatatcta ccatttcaat
     3781 aatctgtgat aattttgacg aagaatctgc aacatttgca ctgagcaact ctatagctga
     3841 agataattca tctatttcag atatatttat cttatcaaga tttactggtt ttgatggatt
     3901 actgtttcta actttgttgg ctaaaagaac aattggtttt gtaaataatc ttgatgcaat
     3961 gtaaatacct attagaccta ttaataaaga aattgctata gatattataa ctaatttctc
     4021 tacccttttt gaaaatgaca aaagactttt ttcttcaata atacccaaaa gagcccaacg
     4081 ttcattttca aatggagtat tggagttata taaatctaag tattgtacac tagcataaat
     4141 gttgccatta ccacattcat tatctttatt tttaatctta tatatatttt tatacgattc
     4201 cttctgctct aaattagaat gtaaaccatc ccaaaatttt tcttttaatg agtatccatt
     4261 tgaaattaca ttttcaaagg ccatatcatt atttctatct acacctaaaa tataattacc
     4321 ctttttattc ccatttatct ctgagtatgg aagtaattct cttaagtaat ttacacttaa
     4381 atctattcca agtactccat atacagttcc atcttcataa ataaggggaa ctgtatatgt
     4441 cataagccta tcacctgcat ttgtcaataa aaacttactt ccccagtatc ctaaattaga
     4501 acttgataca tttggattat caattgcagc tctaaatggt ttataaaaaa aatcatctcc
     4561 atcttgctca ttttcacttt taaatgaaaa cttaggttcc caataactgt ccatagatat
     4621 tccaaaactt cttgctatat ctgatgaacc tctctcaatt aacaaatcag aattatcatt
     4681 tggattaaac ttagggtcta aatcccttat atacaatcct gtcctactta tttcaccttt
     4741 actatctatt gacttatcgt taatattttt gtcacttagg attataaaag ctcctgtaac
     4801 agaatttttt ctcaaaagat atattaaatc atttgatatg ctttttataa tatctttgtt
     4861 aatttcttca ttgttgccta catcctcaat taaggcattt ttttcattta atactttttt
     4921 tacagaggaa ttaattgtag cttccgtttc acttgtattt gaccatcttt gtaacatttc
     4981 attttgtata taatttttac gattaattac tctttcattt aagatgtcaa acgaattatc
     5041 atttaatttc ttaatagttc ctccccataa aattgtggca caaaataaac ttgtttgaat
     5101 tagcataaca ataattaggg gaaatatcat ctttttcatt attgaattgt tttttcttag
     5161 attgttttcc atacccattc cacctatacc tttgtgctcg tcaacacact aactgccaat
     5221 tcatctattt ttttatagca ttttcaagct cagccttaaa actttcaaac caagttttaa
     5281 aatttttatc attattaaat tgtgctactg catccttctt agaagttcca ctttttacta
     5341 attcatttat tttatttgaa tcttctttag ctttatcaag cattgaatat tcaagtattt
     5401 gtctagcttt agttccacca tcaaatgctt tattagtata taaattacaa ctgttaatct
     5461 gttcaataga tgaggctatt gaaccttcaa ctttatcaga taaactcaaa ttatctttct
     5521 tcataacttc ttttataaca tccaaatcat tcgttttttt ctttactggt aagtatccag
     5581 aagcaataga gaattttata ttaggttctt tatctgtaaa ccattttaaa aattctactg
     5641 atgcatattc ttcttcttta gtagattttg ttacagccat tcctgcacct tgttgtacag
     5701 catattttcc ttctccttta aaacgtggtg cttgaaaaac agctgtttca atattataac
     5761 tctcattatc atttagcatt actctatctg gaaagtatgc tgctcctgaa ctagaaccta
     5821 caagagctaa tatatcacct gtttttgcat catcagaacg aaacttacca taagaagcaa
     5881 aatatccatt tatatatggt acatagaaat tatcccatag ttttttcata actttttcat
     5941 caatatttag tgtaacttta ccatctttaa cagaaaaaat ttctactcct agttgtttac
     6001 ttcctataat aatataattt gctaaagcat ctcttccaaa aaatgcttta ccatcattag
     6061 gctctttcgt taaactatct gtccattcat aatatttttt tgatgtctct actaatcctt
     6121 caattgtttt tatatcattt atatttgaat ttgtagcttt tgcaaattta tcccaatcag
     6181 ttttatttaa catcaaaatt tcagttgatt ttgcaattgg gaaaagttta aactcatcct
     6241 tagaatttat tctgccttct tctacatatt cgtctatgta ttcatttaat tcttctttac
     6301 ttatgtactt atccatatct actagtaacc ctaatttatc catttcatat gcagtatctg
     6361 agtatgctgc aaatatattt ggtacatctt cagaaccaac ttttttattt gcagaatcta
     6421 aaactttctc tgataaatca actacattac cttgactgaa tgcttcaaca ataattcctt
     6481 tttcagcacc tacagtgtca ttaaattcct taacaagctt atcaaaagcc attttctgct
     6541 gtccattata ataatgccaa atttgtattg atgtcggatt ttctgggtct aacttataat
     6601 tatcttcctt ctttaaacta catcctgtaa gtaataaaac aatcattgat gtgataatta
     6661 attttgcaaa cttacaccta ttcataaaat ctactccttc aaattataaa atttataata
     6721 gcattaaaac tgtaacttca ctatttatat taattataga acgtttattg atatatttaa
     6781 acaggattta caacatattt aaaagcaatt aagaaacata ctattacaaa tatagaaaat
     6841 aaagctttta cataattttt atttctaaat gcgccaaatg acaccaatcc ttgttgaata
     6901 gctactgtaa ttactaaaag tatcattatt atattaagtt gaaccagttt aaataagtaa
     6961 agcaatgaaa acactactag aagtacacat aatacaatac tagatttatt aataatatca
     7021 tcaatcttat ctaccttctt agattgctta ttaactttat ttaatttttc tttatttaaa
     7081 ttctcaactt ttttattata cctttctctt gttttatctt tatatttagt atttttattt
     7141 ttatttccca tttattttcc atcctctata aattttttaa ttgagcttat acattattta
     7201 aatattatat atttaagata ccatatattc ctacaaaaag gtaacttact atgtatctat
     7261 ttttgatttt cgcataaaaa agccatctcc tttagaatta attctgtatt gagacagcta
     7321 atttaactat ttatcctttg aaacattttc tacattatca tatttactat caactaaaac
     7381 tatatcaact cttctatttt ttgacctacc ttcttcagat gaattttctg ctattggtct
     7441 aaactcacca tatcctacag cagataattt atctggtgct atacctgcat tgtcaataag
     7501 aagttgaaca acattggttg ctctcataac tgataaatcc caatttgact taaactctga
     7561 attttttata gacatattgt ctgtatggcc ttctactctc atgtaactat tcatttcatt
     7621 taacatctta cctatttgaa taatcttatc tctagaatta tccttcacaa tcgctttccc
     7681 tgtatcaaat agtattgtat ctttaaggct tattactaat cctctatctt gaactcttaa
     7741 tgatactgaa ttagccatac cattttcatt taaatacttc tggatttctt caccaacttt
     7801 tttaaactta gcattttgta aagtatctaa atcattactt cctggttcaa ctactggttc
     7861 tatcttgcta ttaccacctt caataactcc agaagaacca ccaaaagcag aatttagaga
     7921 ttgagataat tgtttatatt tctccgcatc aacattactc atagaataca ttattacaaa
     7981 gaaaatcata agtaatgtta tgaggtctga ataagttaaa agccatcgct cattgttttc
     8041 ttctttttca tcttcatcat acaacataaa cattccatcc ttttatatta ttcagctcta
     8101 ccaccaattt cttcaagttc aattagttct tttgaattta aaaatatttt taatttttct
     8161 gtcattaaat tagaattatc accttcaagt atggataata ttgcttctat caccaaaagt
     8221 ctttcactta tttcctgttg atttatagcc ttaagttttg atgctattgg taaccatatt
     8281 aaattggcag aaccaacacc atacaaagta gccaagaatg caacagaaat ttgaggtcct
     8341 aatgcatctg gattacttga aagattacct aaaatatgta ctaaccccat aactgtacct
     8401 attatcccca gtgtaggtgc atatccccct gctgaatcaa aaattgctat tccttcttta
     8461 tgtcgttttg atgttatctg tatctgtgat tccaatatat ttcttatagt aactgcatcc
     8521 actccatcta ccataagttc aagccctttt ctaataaaag ggtctaattc atcattctca
     8581 cttatttctt tgtcaatact aagaagcccc tctttcctag cctttgttgc aatttctttg
     8641 aaatagatta tattttctat gttattaggc tttttatttt taaaagctaa taacattatc
     8701 tttatagact ttttaaacac agggaatgga gtcgaagctc caacagctcc tatagttcca
     8761 ccaaacacaa caagtgctga tgaaaataca aataatgctc caacatgtcc cccatcaata
     8821 acaaaagcaa ctaccaaaga tgctatcccc actatgaaaa aaattaaagt tgtcatctat
     8881 tccctccttt ctttagttat acatatctaa tcatttatga cattataaaa agactatctt
     8941 taataaaaat agcctttaat aattggcgga gaaggaggga ttcgaaccct cgcaccggtt
     9001 acccagccta atcccttagc aggggatcct cttgagccac ttgagtactt ctccatatct
     9061 ctatactagt aaattatata ataaaatcat attttagtca attgttttat aaaatttgag
     9121 ttattttgaa ttattaaaca tctattttta cttttttgct taaatgtaag ttttaaatat
     9181 ggtaaaatta tcttgcataa cacttataat attatttgat aaatatacat acattaacaa
     9241 atatacctaa actctgactt aattgtaagt tatgccaaaa ctaaaataat actagtccaa
     9301 ggaggatatt ttatgttacc acaggaaaga tatgagaaga ttttaagtat tctcaaatca
     9361 gataatataa ttaaaataaa tgacatagtt caaatgtttg atatatcaat agaaactgca
     9421 cgaagagatt tagcccatat ggagagtatc ggtcttttaa aaagagttta tggtggtgct
     9481 ataccagtta tagcaagtag cgtagaactt aactatgtta aaagaaaaaa aataaactca
     9541 aacgataaaa aacagattgc tattaaatgt agtgagttta taaatacaaa tgatactatt
     9601 tttatggata ctggaactac tgtacttgaa gtttcaaaaa atttgaaaga taaaaagaat
     9661 ctaactgtaa taaccaactc aattttagtt gcaaacgaac ttatgaatac taatataaaa
     9721 gtatatgtgc ttgggggcta tcttagagaa actgaaggtt ctttttctgg tccgcttaca
     9781 ctgtcagcta ttgaacaatt taatgttgat aaagctattt taggcgctgg aggtatcact
     9841 cttgaaaatg gaatctctga ctatcactta gaagaagcac ttgttagaaa aaaaatgatt
     9901 gaaaggtcta gaaaatctat agtagttgtc gaacctccta agtttggtat aaattcattt
     9961 gcatcagtag ctcctataga acatatagat actattatta cctctgctag tatagaccat
    10021 aatataataa tagagttttc tcaaaaagga atacctttag tcatagctga taattagctt
    10081 atatgattga tttttagttc cttgatgtaa taaattaaaa ttaaggagcc aaataattat
    10141 ttatttagct ccatttatgt ctaaactata ctttatattt taattatgta actactttca
    10201 gttagcttat catttttagt tatttgattg ccattttatt taatttataa gttttagtta
    10261 ttcgattatt ttttaattgt ctgtcttttt aatttatttt tagttattta aactcctact
    10321 ttaaaataat ttaaatttga cttaagtatt gtttattcta cactgcatct ttcttcattt
    10381 taatactttg ttcttcttcc aaggttaaat cttttgtcat aagtatagca agtgcaattg
    10441 ccaatactcc tccaaccaat tttccaatta caacaggtgt aatcatctct ggaactactc
    10501 ctgctgtaaa tcctaaatgg tcccccaaag ctgctgtagc acaaaccagc caagcagtat
    10561 ttactacttt cccctttgga ctcatatctt tcatcatttt atatactgga atactattgg
    10621 ctaaagtaaa aactaatcct gctgcactag tggcatccat acctaatttt ccacccacta
    10681 ttgtaagtgg tttattaaga actttaacta ataaatgtaa tattggaaaa gtacctaata
    10741 acacgattgc aatacttcca attacttgaa taccttctct tataggtgtc atgcctttta
    10801 taagtactat acctgttatt tcttgaaatg ctgctgctcc caaacctatc gttatcaata
    10861 tagatataaa ttttccaaaa attaatgacc catttatcat aagcgtagaa ttaaatttta
    10921 aacctactgc caatatcact gccaatatta tcactggtat catatttata attaccattg
    10981 ataagttaaa tcctgctatt aatcctccta taatacctcc aaaaggtatt gttatcaagc
    11041 ctatcagtaa cccttttgca aagaatggtc tgtcttcata ttctataaga cctagtccta
    11101 ctggaattga aaatactaat gtacatccaa gcatggatga cacaaccaat cctgcaagca
    11161 ttcctgcctc tttattgtgt gcaagttcca tagccaatgg ataccctccc atatcattcg
    11221 ctaaaatagt tgcaaacata gatgggtcag ctccacagag attgaagatt ggtattatta
    11281 taggacctag tacatctgaa attactggag ctaaacaaac tataccaacc attcctaaag
    11341 ccaatggacc cattgaatta aacccttctt caaactgctc tccaagtcca aatttatttc
    11401 caattattct atctaacgct cctaaaagca ctcccacagc cattatgtat aatataattt
    11461 tatcaaaggg catttttaat tcctcctata catgtaaaat actctattgt aacttaatta
    11521 gcacacacct ttttggtatc gttttcccaa ttcatagctc tttttactgc ttgattccat
    11581 ccaagtaata tttcttctct tttttgatta gtcatatttg gaatatattt tttgcttatt
    11641 gtaaattcac ttctaatttc atcaatactg ttccagacac cacaagaaag acctgccata
    11701 tatcctactc caagtactgt cgtctcagga ttttctgcaa tttcaattgg tatacctaat
    11761 atgtcggatt gaaattgcat tgtaaagtta tttttagtaa ttccaccatc tactctcaac
    11821 atttttattt ttttccctga ggctaagttc atagaatcaa caacatcttt gacttgaagt
    11881 gcaacagact caagagtcgc tcttactata tgctcttttt tagtacttcc tgttatccca
    11941 accattgtac ctctagcata ttggtcccaa taaggtgctg ctatacctgc aaatgctggt
    12001 acaaaatata cacctccagt acttgatatg gaatttgcca tgtcatcagt ttctgaataa
    12061 ttttttataa ctccaatgcc atctctaagc cattgtattg cagcacctgc tatatacact
    12121 ccaccatcat aagcatatgt aactttatct cctattttcc aagcaacagt tgtcaaaata
    12181 ccattacctg ctatatttag ttcctcacct atattcatat ataggaaaca tcctgtacca
    12241 taagtatttt taaccattcc tggttcaaaa catccattgc caaataaagc tgctggttgg
    12301 tctaccattg aagcagtaat ttttatagaa accccacaaa atgcctcctt atctgtagtt
    12361 ccaaaatcac cactcgtagg cattatatct gctaaaatac ttctaggtat tcctataatg
    12421 tcaagcaact ctctatccca atccatatct cttatattaa aaagcattgt tcttgctgct
    12481 gtagatacat cagttaaaaa agattttcct cctgtcattt tccatattaa ccaactatct
    12541 aatgttcctg caagtacttc tccattttta attttttctt ttacaccttt tacattatct
    12601 attacccatt taatttttgt acctgagaaa tatgggtcta taattaaccc agtcttatct
    12661 attatcttat tttctaaacc tggtatattc tttaaatttt ccacataatc ataagttctc
    12721 ctgcactgcc acacaatagc attgtaaagt ggtttacctg tatttttatc ccataacatt
    12781 actgtttcac cttgattatc tataccaatt ccttttatat cactttcatt gattccattt
    12841 tcaaaagcct gttgtaatac attattactc acagtttttg tattattcca aatttctata
    12901 gggtcatgtt caacatatcc agattgtgga tatatttgag tatgttccat ataatcgcta
    12961 aaaacaaact ctgatttttc gtttatcaat actgctcttg ttcctgttgt tccttggtca
    13021 attgctaata catatttcac tttaaatgtc cccctttatt ttcagaatta acaaattctt
    13081 ttttattttt tgaatatata atctattcaa gttatttata ttttatatca tcatgtggat
    13141 ttatgcaata ttcattacaa tattttttgt tatttttaat tatagtgatt aaaaaagtat
    13201 ttttattgaa atattattgc aatattattg caatatttat tattttattc tataatctag
    13261 ttaaaataac acataacatt ttaatattta cacataatca gtcattcaaa aatttaaatt
    13321 taacttttta aggaggcttt acaatgaaaa aaacatatct attcagtcct ggaccagtaa
    13381 tggttacaga taaagtcaga aattcacttc ttcactatga tatatgccat agaggaaatg
    13441 agtttatgaa tttattcaaa gatacacaac aaaaaatcaa taaactatat aatgcaactt
    13501 ctaattacta ctcattaatt gtatctggct ctggaacatc tgtaaatgaa tgtgtactat
    13561 cttctatctt tgacaaagaa gatgcagttc tgctagttag taatggtgtt tttggagaaa
    13621 ggctagaaga aatattatct acttataatg taaaaacata taaacctaat tttgaatggg
    13681 cagaataccc ggacctagac attttagaaa aatacataat tgaaaattct gatataaaag
    13741 taattgctat ggtgttccat gaaactagtt caggaatgat aaatcccgtc cctgaactag
    13801 gaaaacttgc taagaagtat aacaaaatat tttttgtaga tgcaataagt gcctcagcag
    13861 gtgaatatat tgatgtggaa gagtttaata tagatataat aacaggtgtt ggtggcaaag
    13921 ctcttggagc atttccaggt tctgcatacc tttgtgctaa agagagtatt ttacaaaata
    13981 ttaaagaaaa tcaatgtaag aatgtgtatc ttaatcttta taaacactat aaagttgcta
    14041 aatcatcttc tcaaacacca aatacaccta atgttacttt aatatttgcc cttaatgaag
    14101 ctttaactga atttttggaa gatgacagta aaatagaaag atacaaagaa tgttctgcta
    14161 ttttaaggaa cggtatggaa gaattgggtc taacattttt acttccagat aagtatatgt
    14221 ctaatacagt aacatctgta tttctaccta aagaaatcaa tgtaaatgag tttatattag
    14281 ggctagaaag ggaaagtggc tatgttgtct atccaggaaa aggcaaattc ctagatgcta
    14341 acatattcca agtagcaaat atgggagaaa tatatccaga cgattgttat aaattcttag
    14401 atatcttaaa atcaaaattg gagataaaaa aagttatata acagtttgtt tgtaaaatcc
    14461 tacaatatat cagtataaat taaatagact gattttttta aattttccta taataaatta
    14521 tattttatat ttttgtataa gggtgaacta taatatgtaa agttcacccc tatttcaaat
    14581 ctttttcctt caatataatc tttttctttt aatttatttt atatttttga cgcaatcgtt
    14641 ttttattcaa tcattttata ttcttgatac aatcattctt acacttaatt attttttgtt
    14701 tttgacacaa tcgttttttg ttgaatcatt ttatattctt gatacaatca ttcttacact
    14761 taattatttt ttgtttttga cacaatcgtt ttttattcag tcatttaata ttttcaacac
    14821 aatcattctt acattctatt attgtttgtt tttgatacaa ctgtttttat tcagtcattt
    14881 aatattctca acacaattac cttttcattc aatcaaagta atctttaagc attatttttc
    14941 aagctcatca aagtaattat atacgataac ctcacctgtc tctttatctg tagtctttat
    15001 aatgccctta gctccattta cctcttcttt tgtaaattca tcatctaaca caactttgaa
    15061 tttatcttcc tttccataac cttgttgaaa ttcatctctc aaaattgcat ttttttctgc
    15121 ttttggcttc aactcaaatt caattccaga tttcatttca tcaaaataat ttaatactac
    15181 aacctcatta ttcattttat tagttgtctt tataatccct ttagctccat ttacttcttc
    15241 ttttgtaaat tcatcatcca atgttatatt aaatttgtct tcttttccat attcctcttg
    15301 aaaatccctc ttcatttcat tgtatttagt ttcaaactct ttactcttgt ttaattccat
    15361 agctgataca tttgtcaatg atgctccaat tgccattact gttaatgcca ttactatacc
    15421 acttttaaat ttttttactt tcatatctta cctcctaaat taaatgttct caatgaaatt
    15481 atataaagaa aatcctaagt tttacagttt cattcttgta actaaaatta ctattatgta
    15541 accaaacttt ttgcttcaac tattccttaa aagttgcttt tttgaggaat agttgaagtt
    15601 aaataattag attttatcta tatttataga tttaaaatca aatttatcca tataaataaa
    15661 tacttatttt tgcaaaataa aaatttttaa ttactccaat ccttcttatt tacttaaaat
    15721 tctatgataa gaaatttaaa agtctttacc ttctttaaca ataatttatt tacttttaac
    15781 atacaaaata gagagagtat atcaaaatca ttttactgaa tattctaaaa ataagtaccc
    15841 cctattagta tatatattta catattaaaa aaaagtttta aataaagtta tattttttac
    15901 aacagttttg taattaaaaa catattgaag gtaaatttaa aggtactata tactataaat
    15961 atgtcgacac aataaattta aaaaggagaa ctaaatatga aaaaaagatt aataataatg
    16021 atattgtctg ttgtcttagt actatcaagt atacttacga tatttgcata ttcaaatatt
    16081 aaatataata ataacaaatt gatatattct aatatgatag ataaaaaaac tcaaaatagt
    16141 gtaaaggaaa tcctaaaaga aaataaaatt aatgaaaaag acatagatac atttattaaa
    16201 gcagtcaaca attataataa attgcaagtt aaaatacttc aaaataatat aaatatatct
    16261 aaatctggat attcttcaat caatgcaaaa caagttccat ataacctaga aaaattgcaa
    16321 gataattggg tgaaaaaatt ccctgattat atggatgtaa attgtagaat aactgctttt
    16381 agacttttta aagattttat aaattcaaat aagaaattta ctggagactc tattgatttg
    16441 aatgtcgact tggacactat aatgaataat aaagatgcta aatttagcac caaagatgta
    16501 gaaaaattta ttaacttctt ttctgcaata ccagctaaag acacagatga tacaatcaaa
    16561 attgcagaac aaataaaaaa tgaatggaaa aaaagaaaaa tatcttttaa aaataataaa
    16621 aatatatcta ttataaatgg atttcttcgt tatccagaaa ctaaaaacgt atttataggt
    16681 catacaggca tatgtattaa aactaaaaat ggcattctat ttttagaaaa atatggtgtt
    16741 acatcacctt atcaagttac taaatttaaa aataaagaag atgtaaaaaa ttatatgttt
    16801 aatagattga agatgtcaga aggtgaaatt gaattacctg accctataat aatggaaaac
    16861 gataaactta tgaaataata actttaaacg caaaatagta tttataatta ctaagtcaat
    16921 ataatagaga aggtctatag atttgtgtaa atacaagctt atctacaatc ttatataaga
    16981 atgtttatat aaataagata gattaatcct aagtcctatt attctattga attttattat
    17041 tattttattt agattctatt gttattttac ttagaactta ttattatttt atttaagttc
    17101 tattactatt ttcaatcata taaatttaca aaagactgta cataaattta ttttatacaa
    17161 acttatctac agtcttttgt gtgaatatat ctaaataata tcgtatttat ttatttcctc
    17221 tgcacagtga gaatcagaac ctaaaactaa tggaatatcg tattttttta ccaactctaa
    17281 taagtatcca tctatataag gctctttgca gtactctttt ctatttccag aaacattata
    17341 atcaagttca taaccttttt ctttaattaa ctttattaac tcttcaagaa ttgtatttcc
    17401 tttataatcg tatggaaata cctgattaaa ctttctaact aaatttaaat gacctattct
    17461 ttttggttta tgtataccta aatctgaatt tacagctttt atcaatgttt cataatactt
    17521 attataaaca gcttcaatac ttcctaactt gtcgattaaa ttttgaaact cctctacact
    17581 gtaatctaca caataataat catcatcaat tttaattata tgtactgata aaagagaatc
    17641 ctctaattct tctccatatt tatttaaatt cgcctttatc ccttcttcgt atccttctat
    17701 aaaatccact tccgacccaa catttatctt gattttaccc tcatactttt ctttcaactt
    17761 cttgacatca ttaaaatact ttggtaaatc ttcaatactc atacaactat cattttcagg
    17821 agcagggtct ctaaatccat ttgcatacgg aaaatgttct gtaaatgtca tttctgttat
    17881 tccaacatcc attgctcttt gtacatattt ttcaaaatca tccttacttc catgaggaca
    17941 ataaggtgaa tgtatatgac catctttcat aaataattca ctcctttttt aaagaaaaaa
    18001 tctaatataa atgcataaat cttttaactt caaaataata tttttaatca tgctaatttt
    18061 tattatatct aatattagag taattttaaa ctgcctttta agtatcttat cattagtact
    18121 attttcaact taacattaat attatttttt aacttaacat taatactatc tttaaatagt
    18181 aatactcaat atataattac ttaacataaa ataactcaaa ttgcttgatg aggcttttgc
    18241 agtatttaaa gaatatataa ttatttaaca ttaaattatc caatcttgta ataaaattta
    18301 ctcattaaag tatattacag aaaatacctc aaaataaact ttttagttca tgagatactc
    18361 ttttttacat gaaattagat atatttttat catttcaaca atgaaaggga ggcttatatc
    18421 catctttaga caacccctta gataaaattt ttaataatta agcatcacaa aatataaaaa
    18481 tcgcttatat caaacgtata agcgattaat tatatttaca tatgtttttt agcctctaac
    18541 aacattttat tagcttcttc catatactct atagcctttt caatactctt tgctgtttct
    18601 tcttttccaa tagctctagc cttttcaacc cactctctaa atccttcttc atgagtttca
    18661 ttgtgattta tccaatgtac taaaagtatt tttaaagttt tttcatcctt agactctgat
    18721 gattcctcat gactatgttc gtggtcgtgg tcgtgagcat ggtcatgtgg atgttcatga
    18781 catatatctc catgagtatg ttcgtggcag tggtcatggt catgatgtcc atgatgatat
    18841 cttacaatct ctttatcaaa aatcattagt tattccccct gttattttat attaaactct
    18901 ttttttataa ggtttacaga atcttttaca agtagtgcta atggtttacc ttctaatcca
    18961 actaactcca cattttctgg cataataggt aaaagtatct taagtgcctc actgtctgca
    19021 atagcttcac ttatattaca agtaacttct cccatcatag aattaggtat tattacagaa
    19081 ataggtgcaa ctattatatt tgctttttta gctgatacta ctatagcatt ttcccctgta
    19141 gctcctttat tagcatgagc tttcatcata gaactagttg ctatagaatt agtccctagt
    19201 gcataaattt ctacatatgt aggtaactct tctctcaaag aagaaactat ctgggctcca
    19261 atacctccac ccattccatc tatcactgct attatcatat gaaattcccc tattaattaa
    19321 ttttcttgta ttattatctt atggtccatc aatcttattt ctttaattat cccatctatt
    19381 atcttttgct ctcctaataa atccgttaaa taaatctttc catcacaagg gtctatatta
    19441 accacatttt ccataacctt ttctaactca ttattacttt tacaaatgaa agctgatgat
    19501 tcacacatat aaatctcctc ctaatatgat ttttattaaa aatcaaaaaa agattccctt
    19561 aggtatttca atacaccaaa gaaaccttca tgatttcagt ttttgtatat tattatacct
    19621 taagtatatc actttaattt atgtaaagtc aattaaagtt aacgcttttt attatactct
    19681 tcattcttgt atttttcata tagttcttta actaattgct tctcataatc agtcaaatcg
    19741 ccctccacaa actcaacatt aaattgttca gctaatccct ttactatgtt tttctgtaga
    19801 atatctatgt ctatttttct gccaatttca ttttctattc cacttgcttt tttagctgta
    19861 aatttcatag ctctttcttt caattctggt gtcttagttt ttattaattt aaacaatttt
    19921 tctacatcaa aatccagtat tattgaacca tgttgtaata aaacaccatc ttttctgctt
    19981 tgagcacttc caattacctt cttattatta atagtaactt catatgatgc atgtgcatta
    20041 aaacatgcag ctgataaatt ttccctactt attctctcgc ctctatttaa attatctgtt
    20101 tctataccac ttagattcaa ccctttaact agaccttcac ttatatatct ataagatagg
    20161 ttaatgctct tatcgattaa aggattatct tctcctataa ttatactata ggttagctca
    20221 ttatcatgta atacagctct tccacctgta attcttctag tatagtctat attcatacat
    20281 ctacacttat ctaaatctat ctcatcttcc aatttctgaa aatatcctat actcaaacaa
    20341 gctggttccc atgtataaaa tctaagagtt ggcttattat gtcctttctt atatgcagta
    20401 aatatagctt cgtcaattgc catattcatt gctccttcat atgatttatt atgtataact
    20461 ctccactgat tcatgattat tacgccccca tttttagtat aaatatatcc tagaaatcct
    20521 ctatagattt atttctctta ttgctaaatc tatctcactt tgatttaaag gtcttgaata
    20581 gttgtatata gtacttcctg gtctttcatt atagtatggt ctattacaat ccttacaacc
    20641 tcttatttca aaaggatagc ctttgtttat gtcttttaaa atatcattgt caagctttat
    20701 actatttaag tatccatttt taaacttaaa atactctttt ggataacctt tatttatcat
    20761 ataagacatt aactgtaccc ttctataact ttctatgctt ggttgactta tcttttccat
    20821 tttagttcct ctaactggag taaatgcaaa taaactaatc gttacatcat tttcctttaa
    20881 ataagtatat aaattgtata tatcttcatg actttcaccc ataccaacta ttatatgtgt
    20941 acttatttta ttgggatatg attttgacat ttcagttata aattttaact tttcatcata
    21001 gttatttcct tttatttttt catatagttc tttatttgct gcatctattg ctattcctat
    21061 tttatttact cctgctgaaa aaaacttctt tatctgttca tcattttcta attttgcaga
    21121 tagtgaaatt ggcatgtcta tcttaccact tatatagttt ataaagtcta aaacagagtt
    21181 atgtgcttct tcacttgcca ttgattgaat acatattctc tttatatttt ttcctttata
    21241 agcttttaaa gcatctaata tttcttcttt tgaaaactct ggccaaatta ctcttgataa
    21301 tttatctttt ctagtactac tttctattga ttgagaacaa aaactgcatt tatttatgca
    21361 tttttctcca atcattatat atgctgtggt aggaggtata tcgctttttt tgtttaatat
    21421 cccaagttct attgctgtcc caactgatag tctaatcata aaacgcaaca ctccttacac
    21481 tcatttattg tcatattcat ttctatagcc ttattctttg cagaccttga tggtaatact
    21541 attctattga ctccacacat tatagataat tggtctagct cttttctata cactcctctt
    21601 ggtctcatac aacctagatt aatttcagta tctggtaaat taattctagc ttcacataaa
    21661 atatctgcta ccccttctaa ctcaggaggt tctacatttt catactcagt cccttttgtt
    21721 ggaataagga ctataaatgt aagcttatta ggaggatttt tttgtaggta ttctataatt
    21781 tcatattcac cctttatttg tcctcctttt aaaccaatac atatatgtgg tgctacttct
    21841 gtatgttctt gtattgtatt atagacttca atataatctt ctttactctt tttcatctta
    21901 taaacttctc ttattgtctc atcatcaaac actaaatcaa aagaaactat atctaaatac
    21961 ttacacaact ctacaatgct atctttatcc ataagaccta aatgagcatt caatctatat
    22021 ccttgctctt ttaagttttt aatggtatta atatgagtat ttatagggac atctccctca
    22081 taactacaac cgccactcaa tagaaagcta gttatattcc ttgattgtac tttttcctca
    22141 tattcttcta ttggcaccat attgtttaag tagtgaccgt tgcaatgggc acatttcaaa
    22201 ctacattgat tgcttgtagt actaagagct agtgtctggt taggataagc aaactctatt
    22261 ttatttccaa aattctttaa tttaatatca aatgctattt tcattttttc agatagattc
    22321 ttattcatag tttattcctt cttaagtaca ttttactctt ctagtaattt ctttgctgtt
    22381 gggaactgct ctatattcgt atgtggtaag aaacatgcag aaataaattc atccatataa
    22441 cttggatata cactaagttc aacataagtt atttgattac ctatttcttc aagtttattt
    22501 tttgcatctt tacttataag agctaagtaa gaaccaacaa gtgaactatt ccctatataa
    22561 gtaaattttt ctctatcgat atcaggaagt aaccctataa ttattgagtt ttctatgtct
    22621 aggttgtttc ctataccacc tgctatataa accctatcta atatactaaa atccatacct
    22681 aaactatcta caagagtgta tgcgcctgaa tatatagctc ctttagctct gatgaaatta
    22741 tctatatcaa cttcattgac tactatatca ttttctaaat caaattcttc tttaaatgct
    22801 aatacatact ctcctatctc atgctcatta aatctaactc tcttattatc taaatccctg
    22861 tatattttac ctcttctatc tattacacct ttagtaatca tttgacagat taaatcaatt
    22921 ataccagaac cacatatacc aactggtgca cattcatcaa taatctttaa tgttggctct
    22981 aaagtatctt tatcaataat tactttctct attgctcctg ctgatgctct cattccacag
    23041 cttatccctc caccttcaaa agcaggtcct gctgaacatg cacagctcat catataatct
    23101 ttatttccaa atacaatctc tccattagtt cctaaatcta taaataaaac attttcttca
    23161 cttgaccata tccctgctga taatactcct gctgttatat ctcctcctac ataacttgca
    23221 acagatggag ctaagtacac atatgcactg tcatttacaa aaagaccaac attttcaccc
    23281 ataagttttg gagattttaa aaatggtggt atgtaaggtt cttgtcttaa gaagtctgta
    23341 tacacaccca agaataaact tgtcatagtt gtattcccag cagccactaa tgtaactaca
    23401 tcttccttat ctattccatt tctttcatat attgttttta atagtggatt tatagtttcc
    23461 tcaactatag ctttatgaag tgtctctaat ccatttttct tagttgaata tataattcta
    23521 tttataacat ctgctccata ttttatctgt gcatttccag aagatgcttt atctactacc
    23581 tcttttgagt ataggtctac taaacaaaca actactgaag ttgtaccaat atctattgca
    23641 actccgtata aggaattttc tttatttcct tgttctatat ttatgattgt aagtttcttt
    23701 tgtttttgaa catatgttat tgtaacctta aaatcactct ttctaaatac agttggcatt
    23761 tttctaagta tatccaatct aaaatctatt tcattatatc ctaggttatt tctaacatat
    23821 ctttccaatc tatctacatc acttatatta tcatctaaat ttggttcttc catttctatg
    23881 tatttctttt ttatatttgt tttaaattga aggttatgtt cttcaattat ttttttagct
    23941 ctttcaaaaa tttccctatc ctctttttta ttactacctt ctatcttcat accatgcata
    24001 gaggatgata ctttagaagg aacttcaatt tctatatctg atattacctt tgtacaacaa
    24061 gctagtatat aaccttgctc ccactcatca tctgttatat gtcttgtttt ttctgtatca
    24121 acttttccat tcaataattt tactttacac ttcccacaag acacgtttcc attacaagga
    24181 gcatctatga atatatctgc atttcttgca acctctagta atatatcccc ctcattacaa
    24241 taaacctctt tattgtttgg tgtaaaactt acttttatca tgttatccct cccaatttgt
    24301 tctatatatg ataaaaggaa agtatagcta aatttgcgtg cactcaaatt ttactatact
    24361 ttccccaatt ataataatat cttaatcttt aattattcta atccagcctc gtattcttta
    24421 tcacttaata acgtatcaag ttcagccaca tcagctagtt ttacttttaa tatccagttt
    24481 tcatatgggt cttcatttat acattctggc tcatcttcta atttttcatt aacttccaat
    24541 acctcaccat taacaggaga tattaaatca gaagctactt tagaagactc aactactcca
    24601 aaatctactc ccttagttac agtatcttct acttctggtg tttcaacaaa caatatttct
    24661 cctaattgat cttgagcata gtctgttata cctatatata caacatctcc atcaattact
    24721 tttacccact catgatcttt agagtatttt aattctggta ataatttcat ttcgatagac
    24781 ccccaatatt atattattga ttaaattact taaatcatat taatattata tcatattatt
    24841 ttaaattttc catcaattac attacacttt ccatagctaa tgctggatga cctttttctt
    24901 ctaagaaagc taatactcct tcagaatcgt cagctatagt ttcatcacaa accatatcac
    24961 agaagttttc tattccagtc atttctttaa ctgtagcatt taatttatca gctacataat
    25021 cttttaattc ttttggcatc caaactattc tttctggtcc accttctgca taagcgaatt
    25081 ttttagaaga tatgaaatgt cttccgtgtc ccataaatcc tggagtttga actcctccac
    25141 ctgtcataga agcaagttct ccgaaagtca taccaactgg agtaacactt gcaaattctc
    25201 tgttaactac aacaaatcca tttgcttctg gcattatacc acagatacat tcgaagcatc
    25261 cacaagaagt cattgggtct tctagtatag agtataatgt aacagattca actgcacctt
    25321 gagatatttg gtttacagtt tcatttactg aattccatac acctgttcta tcatctaagc
    25381 actcgccttt ttctattggt tgacaaggac ctgttgggtc taattcttta gtagccttag
    25441 catctaacca gctaactgct ccacaaagac caagtctttc aggagtaaca acacaaacgt
    25501 gagctggtgc aaatgattga cataagttac aagaatagaa agtgtcaaca ctttcatcaa
    25561 ctaatgaagc taatctttca tctctagcat tgtacttagc tattgcttca ccttttaatt
    25621 cagaaacttt ttcagcatct gtaattatag ttatttcaca cttatcaact actgattcaa
    25681 attcatctaa catcttagca tataaaactt ctcctatatg ctcaagtcta aatcctttat
    25741 caaatgcatc tttagagatt cttacccaag ccatatctct ttgtccaacg tgcattacac
    25801 cttctatata gtttaagaag tagtggaaac gtctttcaag aactggctcg aaatcttctt
    25861 gcatattttt accagctatt ttaactatta cagcaagtgg taatcttaat acaccatctg
    25921 catcaacttc atctatattt ggtcctatta tttctatctt atggtcttca acttctgttg
    25981 cttctttctt aacaacaagc tcccaagctt cagttctgtt tccaccaaac tcagcaaaca
    26041 tatcgctctt tctaattctc tcaccttcaa atgctgctgc aaatgaaact gggataggta
    26101 tttcagtaac ttttattttt attcctctag cttctaatga agtagctact atcttatcgt
    26161 aatctttttg agttaatagg ttcattggaa cttcaagaac tgtttggtca gttataactg
    26221 ggaatcctaa agctattgct ccagcacctg cagatacaac taattcactt aatggtccaa
    26281 atgcattaac aaatgcagga actctattag ctgtgtactc taataatccg tttaaatcac
    26341 caggagttaa tccaccgaat attaatgctg ctcttactgc aacagataca acgtgtataa
    26401 cagaagttac atcatatcct agaggtataa ctcttagctc aagacccatt tttactccag
    26461 cttctatagc ttggtctata actttaccaa ctaagaaagt tagtaaacct ttagattggt
    26521 aatcttttat aacttttgct gctgattctg catctggaca ctcaccaaga acaactgcaa
    26581 caccaggtat atctcctgta actaatggta caccaagtga tctgattata gggtcagtta
    26641 tatgacctgc acatggctca ctatatggag catccatagt tgaatatttt aatgcttcaa
    26701 ttacttctgc tgctaaagct gtagctaaac cagcattaaa agcatgttct agtaaatgag
    26761 ttctatttat taaagatttt actacttcta aagcaccttc taagtcccct aaagtgttca
    26821 tcttttgtcc tgttgcagca tatatacaag gtaatgaata tgctgtatca gggaaagcaa
    26881 ctttatgctc ttttccattc ttttctatag cttcagctaa catagcttga gccgcaccta
    26941 aagcttgttc tgaccctgta aagattatat tatatagatt cattctattt ccccctttaa
    27001 tatttacacc tattttttat taactatacc ctaaaaaccc tagaaagcaa atccaccttc
    27061 atttaactct aagaaagagt ctgcatataa agatgccccg ttgattatgt cacaagattt
    27121 tacagttttc attaattctg ggtcacatgg attcattata gctgaactaa atccattagc
    27181 cattgccatt gctaagaatg cactatctaa aacaggtcta acatgcttag gacatccatt
    27241 agatacgtta gataatcctc ctgtagttaa aagtcccatc tcagtcatca tttttatagc
    27301 ttctaaaact tctacttgtt tttcttgcat accttttata actaagcata atgggtcaaa
    27361 tagtatatca gttggctcca ttcctaacat taagcctttt tctaatattt cttggcagta
    27421 agccatacgc tcatcattat ctcttgggat accttctttt gcacataaac ctataaccat
    27481 tgcttcgtat tcagctgcta tatctattaa atcaaatctt gagccagcat cagcagagtt
    27541 tattattggt tttgcattag ttctatcata aactttaagt ccagcttcga tagctttttt
    27601 attagctgta tctaatgcta taggaacatt gttgaactca gattgaagta atttgattcc
    27661 ccatgtcatt atttcttcgc catctctttc ggcaggtcct atattaaaat ctatatagtg
    27721 tgctcctgct tctaattgct cttttgctct ttttaagatt ggagctgggt ctctttctgc
    27781 taatgctttt cttattgaag gtgatataca atgtattctc tcacctataa tcataaattt
    27841 ttccatttat aatgtccccc tttaaattta atattaatag ttagttaata ttttattagt
    27901 ttgtttggca tgctttttct tggtatcctt ttaagaactt aggtaattcc atagattcct
    27961 ctggtcctat aactacattc catcctggta agttatcttc tatatcacct ttaagtactg
    28021 caacttttcc tggaatgata aggtctttac aattagttac ttcttctacc ttactttctt
    28081 taatgaaagc agagattgag tttccaccaa atttaccagc agcccaagat gtaagaactg
    28141 aatatccacc tgcatctggt attactaacc atactggaac ttttgatctt tctatatcac
    28201 cagcaactat gaagtaagtt aatgcaaagt caacagtaac taatactgga gaattttcat
    28261 ctgggttgtt gattggataa atctttggtt caactctcat tggtctttgt gggtcagtgt
    28321 atatgttttg tcttagtgca aataatggta atgccttagc ataactaata tcatctatta
    28381 ctattataga accgtatttt atagtgaata ttgatgataa agcaacttcc atcataggat
    28441 ttgaattaga taatctattt gcaaatacta ttgaaggata tccaaatgtt ctatcttgct
    28501 cttttaaagc tgttcttcta acttgtatag cattagtgta agtatctttt atgttttctc
    28561 ctgttacatc taatactaat tctttatatc cagcagcttg aattaattca actgtttcat
    28621 ataattcttc taagctacca gcttttacac ctaatggtaa acttgctcct ttaactactt
    28681 ctatcatatc tttgtagttc tctttagttg ctccatatac cattggtcta gcatctttta
    28741 atacttcaac agcttcttta actacttgtg catcatcaca agctaaaata taagctactt
    28801 ctaatccact gccttttatt ttatctatta attttaaata cgcatcttta tctccacaat
    28861 actctaaaac agccatttca actttcattt gttcaccaat tcttacatag tcaacttttt
    28921 tcatgttagc tattttagca tctactgctt catcactcat acaagtacag aatgaaactg
    28981 catatctatt tctacttact aatgtttttt catgtctaaa taatactgtt tctccaccta
    29041 gttcatactc agatgcacca gcaccaactt ttaaagcttt cattaaaggt gcagtagcct
    29101 ctgataattt tgctatagcg tcatcagaca tatgtgggca tttacctact tctacagctc
    29161 ctgaagcaac cttcatagag aaagccatac aagttgggaa tccacaatcc ttacagtttt
    29221 tctttggtgt taatttaaat atatctaaag cttttaatgc cattattttt tccccctaac
    29281 cttaacaatt taattaagct aatgcgttaa ccaattcttt tatagtttct attgattttg
    29341 gatgacgaag tataactgcg tttgaaccac ctactaaaac acttgctgca gttgaaactt
    29401 ccattgctat agttctttct tctggacaac cccaatctgg ctcatcttct attggagcta
    29461 tagcttcttt aacatgacct acttcaaaag ctactggtgt tatgataggc atttgtaatg
    29521 tcttatcatt ttgaccaaat gctgcaagtc taattctatc catagtagat gcaacgtact
    29581 catatccata accaactgct gaacatccaa cattcataac tatattttca cctttaacgc
    29641 ctagttgagt taacaatacg tttaattgtt tagctaggtt tatatcaaca gaagattcag
    29701 ctcctacttt atgtgcataa gccataccag ctgatgctcc tactcctttg tagttgtctt
    29761 ctactgctga cataaataag cagttatgtc catccaatgt ttgagctaat ttttcaaata
    29821 atttaccatc tttttcatga tttcctgaac cagctacaac taaaggtaat tttattgctt
    29881 caattactgc cttagctaca tcagcacatt catcaactga cttatctaat ccatttgggt
    29941 cagaaccatc aaattttaaa caaataaaat ctgcttgtgt attagcttca acatatttag
    30001 cccactctac tggacaatta gctacatctt tgtataattc cttatatgaa tcagtccagc
    30061 tttcaggata aacgtctgat atttgtatac ctatttttgg agaattacct acttctccat
    30121 caaaactata aaaaggtaat acattttccc cacctaattt aattgctttt tcccctattc
    30181 ctacttcaac ttctgatatt tttccagaat atttttgagt agacatttta aatgccatat
    30241 ttttttcccc cttaagccta ttttaattct aatttagcaa aaatttcttt catagccact
    30301 ttagattttg aatcttcagg taaattaact aaaggaatac ctgtagaatc atattcatat
    30361 atcaattcat ccataggtac aacaccgatt agctctaggt tatgcttttc aatttcttct
    30421 ttaacaccat cattcataac cccatttgga actttattta caatcaaaag tattcttcca
    30481 acacttaact ttagttcctc agctaaatct cttattctag caacagcttg tatgctacgt
    30541 cttgagcaat cactaaccaa taaaagtgta tcaacatgtc tagtgacttt tctacttaga
    30601 tgttccatac cagcttcgtt gtccatgact aagtatttgt aatgaccaga tattttgttt
    30661 acttgttctc taagtatccc atttacaaaa cagtaacatc cttcaccttc tgacctaccc
    30721 attactaata agtcatatcc ttcgccttct tcgattatag aatttaatct aaattgaaga
    30781 tattgtgcct ttgtcatacc accagggaat gcattgccta acttttctct ttgatttact
    30841 tcttctctta tttctcctat tgttgcttca acttcgatac ctaatacttc atttatgtta
    30901 gcattggcat cagcatctac aactaatact ggtccctttt tgtccttaac caaataatca
    30961 atcaaaagcc ctgtgagact tgttttacca gttccacctt ttcctgcaac agctatattg
    31021 tatcccattt taagacttcc cccttaaatc tagccacaga gccttatgta ctctgtctgt
    31081 ctattataat ttaggtacag aatgaacaca ttctccatgt acatcatgaa gtgcttccat
    31141 tagcccttca gataaacttg gatgtggatg tattgcatcc cctacttgtt ctactgttaa
    31201 tcctagatga acagcaagtg acaactctgt caataaatct gtagcatgag gtcctactac
    31261 tgctgcacct attatcttat cagtttcttt gtcagctata acttttacaa atccttggaa
    31321 atgtcctata gcttgtgctt ttccaagccc tctaaaatca aattgaccta cattgtattc
    31381 tacaccttca gcttcaagtt gcttctcagt cttaccaaca ccagcaactt caggttcagt
    31441 ataaacacat cttggaattg ctctataatc tactacctta gtcttaccta aagcattttc
    31501 aactgctacg ataccttctt tagaagcaac atgtgctagg aaaggagtat ctattatatc
    31561 gcctattgcg tatatacctt ctacattagt ttcaagatgt tcattaacta ctacttttcc
    31621 tctttccatt tcaattccta tatcttctac tccagaattg tcaaggttag gtcttctacc
    31681 aacacatact aatgcatatt gtgcttctat aacctttcca ttagaaagag ttgcaacagc
    31741 cttaccatca actacttcac aagtttgtac tccaatacct gtaataacct taattttatc
    31801 tttcttaaat tgtctaagta gttgtttagc tacatctttg tcttcattta atagtatttg
    31861 gtcaaccatc tcaactatag ttacttctgt tcctaaggct ctaaagaact gccctatctc
    31921 acatcctata actccaccac ctactattaa catagactca ggtatctctt caagtccaag
    31981 aacttcatca ctagttatta caacttttcc atcatatggg aacattcttg gtacaactgg
    32041 aacagaacca tttgctagta ttatcttatc tgcttttata gtttctactg ttccatcatc
    32101 tttagttact tctatagtgt ttttatcaat taattttcca aaaccattaa ctaggtttac
    32161 tcctcttttt tcaaataaga actctatacc acttattaat tggtttacaa ctttattttt
    32221 tctttccatt atggcagtga agtttggctt aactgtacca tctatttcta taccaaaatc
    32281 ctttgcttct tttacagtat tcaatactcc tgaagaagca agaagtgctt tagttggtat
    32341 acatcctgca tttaagcaag ttcctccaac tcttcttttt tcaacaactg ttacatctgc
    32401 accaagcata gaagctttta tagctgctac atatcctcct ggtccaccac ctactactac
    32461 tatcttcatc tttaattccc ccaaacctaa tataatctac aatcttgcaa tactataaac
    32521 ctgcggcatc cagtatagca ggtatattct cttctgcccc aattgccatt atatgaacac
    32581 catcacataa atcttcttct tttaattgtt tgataagttc ccctgccatt tttattcctt
    32641 cacttaccca gttttctttt ccagctgctc ttaatctttc gatttgttca tctggaacaa
    32701 atattcctgg tacatttgca gtcataaatt tagccattcc tggtgatttt aaaggtacta
    32761 ttcctgctaa taccttgcaa tccatatccc ttgttaattc tctaaacttt ctcattgtat
    32821 ttatgtcata aagtgcttgt gtttggaaaa atttagcccc tgcttttatt ttctttttca
    32881 tttttaataa ttgaacttct attggagaat attctggagt tactgatgct cctagataaa
    32941 aatctggaga accttttaac ttattaccag ccatgtccgt acctgccatt aaagtctcag
    33001 cagtttgaag tatccccaca gaatctaaat caaacactcc cttagcttgt ggatggtctc
    33061 ctacgctagt atggtctcct gttagtgcca acagattgtt tatcccaaat acacctgctg
    33121 acaacatttc tccttgtata gctattctat ttctatctct ccctgttatt tgaataactg
    33181 gctctaatcc agcatctttt aataatttac atgtagcaag agaagttgct ctcataactg
    33241 ccgattgaaa gtctgttaca tttgctgcat gaactcttcc aactaacggc tttgcacatt
    33301 ctattaaatg tgaaaggtca gtcccttttg gaggagccat ttcagtagta actgcaaatt
    33361 ttccactttc taatgtttct cttaataagc tcatctctaa tccccctttt aaccttctaa
    33421 aaaaaacttt tatgaatttg ctgctgcttc tttctttttc tttgcactta aacttcttgg
    33481 attattttgc ttagaatagt cttttggtgg tcttatttct gctaagttat ctaattgtcc
    33541 tatagcttct agtctttcat atatctttat ccaagcacaa tcattctctg agtttacttc
    33601 acacttacca tttttagctc caccacaagc cccattcata agacctttag cacacatagt
    33661 tactggacat atcccacctg tccaaccaag ttcacagtct ccacaagcct tacaggcttc
    33721 ttcatattct ccaactcttt gtacttcccc tataaacaaa gtattgttgg ctggataaac
    33781 tggcttatct tttaaattct taactattgt ctgtgtacca tctccacatg ctaaagataa
    33841 aactgcatca gcttccttag tctcttcttt tagagccttt aaatcctttt tagactttaa
    33901 aaggttacaa gcagggtcaa gcattacata tcctaagatt tttttgcctt caccttcaag
    33961 agtttccttc atttttaaaa cttcttcttc cccaccactt ttacaagttg ctgcacattg
    34021 attacaacca actaaaacca gcttgtcaaa atcctttaag taccctagca cttcttctaa
    34081 tggtttattt tcagaaatta tcatatctac ttccccccta aaccttccac atatatatct
    34141 atttattgtt ttgtaattta caagccttaa ctacatgttt tgcaagtata gatgttgtaa
    34201 ctgaaccaac ccccgctgga actggagtta tcattgatac tttgtctaaa actgcatttg
    34261 tatcaacatc tccacataaa tttccttctt catcaacatt tataccaacg tctataacta
    34321 ccgctccatc tttaacaaaa ctttcatcaa ccatcttagc tctaccaata gctgctatta
    34381 gcacgtctgc ctctgctgct acacctgata gatttttagt ttttgaatga catattgtaa
    34441 cagttgcatg ttcacttaat aaaagcattg atactggctt tccaactacc attgatcttc
    34501 caagaactgt taccttactt ccttttaaat caacattata gtgttttaat atctcaacta
    34561 ctgctgtagg tgtacatggt ggaaatccac ttttatcccc ttccattacc ttagcagagt
    34621 ttattggact aaaacaatct acgtcttttt ccggtgcaat aacatatttt ataacttctt
    34681 catttaagtg ttttggaaga ggtctaaaac ataatatacc attaacattt ttgtcatcat
    34741 taactctctt taatacatca atatattcct cttgtgttat atcctcagct aactctaaca
    34801 cttctgttgt tatacctatg ttttgacatc tctttagtgc tcctctttca taagataaat
    34861 catcacttct tgccccaact ctaactattg ttagtttagg atttatacct tctttaacta
    34921 gtaaatcgac ttcttttatt aattcttcac taatcttatc agctacaggt tttcctttta
    34981 taatttgtcc tttagttgac attccttcca tacattcaca actcccctac aagcaaactt
    35041 aaattaattt attctatata ccacatatta ataacatagt atctactttc gtcatttaaa
    35101 aattattttc caagagcttt ttcaactttg gcataaactt catcacaaat ttttacacca
    35161 tcttcaacta acttatctac ttctgttttt accttattaa catattcttt atcttgtata
    35221 gaatttatat ttataactac atttaattgt ccaccaagta tagcagctct taaacactga
    35281 actcctacac caacatcact tatagcaagt cttgaacctt tatcaactaa atcttcatgt
    35341 aacttaatag cttcatagca aactcttact atgtttaatg gtacttcaca agcaactttt
    35401 aatgcctttt ccattgtttc tgcttttatt cttttttctt cttctgtttc tttaggaagc
    35461 ccgtaagctt ttgataatgg taagaaacat tctgcatcat catctatcat ttttaataaa
    35521 tctttttcta actttcctgc cttacttaat atctctttta cactttcttc atactcagca
    35581 tacttcttct tccctattgt aaggttacat accatactac ctaaagccat acctattgca
    35641 cctactagtg cagcagctcc cccaccacct ggtactgctt ctttggaaga tagtacttct
    35701 acaaaatcaa cacaagtttt atctgctatt ttcatgataa tcctccttga aaggatgtag
    35761 gaaaatatct caaattgttc gagatatttt acgaactatt ttaagatatc tcctactctt
    35821 tatttataaa ttaatttcct tagaataatc cagctattac tccattttca tcaacatcta
    35881 ttttttctgc tgctggaact tttggtaatc ctggcatctt cattatttct ccagtaagtg
    35941 caactataaa tccagctcct gctgaaattg ttgcttgtct tacagttatt ctaaatcctg
    36001 ttggtctacc taatttagtt tggtcatcag ttaaagaata ttgagtctta gccatacata
    36061 ctggaacttt agtaaatcca agtttttcta agttagctat ttccttatca gcttcaggag
    36121 tataatctac accatctgct ccataaatct tagtagctat agcatttatc ttgtctttta
    36181 tagataaatc atcttcatag cagaatctaa agttattttc ttcttcatcc aataatctta
    36241 aaacttcttt agctactgca attccacctt ctccaccttt tgcccatact tcagaaagag
    36301 caacattaac acctaattcc ttacacttgt cttcaacaag tttaagttca gcttcagtat
    36361 caagtgggaa tctattgatt gcaacaactg ctggtaatcc atatacttga gttatgtttt
    36421 caacatgttt taataagttt ggaagtcctt tttcaagagc ttctaggttt tcgttattta
    36481 agtctgcttt agcaactcca ccattgtatt ttaatgctct tacagtagca actattataa
    36541 cagcatctgg ttttaagttt gccattctac attttatatc taagaatttt tctgctccaa
    36601 ggtcagcacc gaaaccagcc tcagttatta cataatctgc aaagtgcata gccattcttg
    36661 tagctataac agagttacat ccatgagcta tatttgcgaa tggtccaccg tgtacaaatg
    36721 atggtgttcc ttcaagagtt tgaactaggt ttggttttaa agcatctttt aataatgcag
    36781 ccatagcacc attagctttt aattgtctag ctgttacagg ttcaccttcg tagctgtatc
    36841 caactattat gtttcctaat ctttctttta aatcagatat atcacttgcc aaacagaatg
    36901 ctgccattat ttcagaagca acagttatat cgaatccatc ttgtctaact gctccatcac
    36961 cttttttacc cataccatca acgatatttc taagttgtct atcgttcatg tctacacatc
    37021 ttctccaagt tattttctta gggtctattc taagtgcatt tccttggtga atatggttat
    37081 caagcatagc agctaaaaga ttgttagcag ctcctatagc gtgaaaatct cctgtgaagt
    37141 gtaagtttat atcttccata ggaacaactt gtgcatatcc tccaccagct gctccacctt
    37201 taacaccaaa tactggtcct agagatggtt ctcttaaagc aactaataca tttttgtcta
    37261 gtttagcaaa tgcatcagca acacctatag tagtagtagt ttttccttca cctgctggag
    37321 ttgggtttat agctgtagtt agtattaact tagctttttt accagtctct ctttttagta
    37381 gattgtagtc aacttttgct ttgtatttac cataaagttc tacatcatct tcacctagtc
    37441 ctaatttttt tgcaacctct ctaatatctt gaggcttagc ttcttgagct atttcaatgt
    37501 cagatttaaa tcccataact gaatctcctc ccacaaccta aatagattta aaatataatt
    37561 tttgtaaaaa ttgtattaat atgtaaaaat tatattttat aagataatta tacatgtaaa
    37621 attacttaaa aactagctac taagctatta aaatattcaa acttaatagc tagttattta
    37681 tctaattact tacttattac ctatctattg cctatgtcta acctttaacc aacttaaaat
    37741 cccaaatcaa aatttctatc tattttattt atccttaagt gacattaatt tatcttttat
    37801 tgctttaatc tgttctttcg ttgtttcact agaatcatat ggagattgat tagctctatc
    37861 agaatcaata acatcttggt tataataaat aaatcctaaa gattctccat cttctaagtt
    37921 ttggattata aattcttcat cttccttatt tctaatttta tttcctacta caaatacttt
    37981 atttacacct atatcatgtc caagtttctt aacttttcta taagtctgta gacttctctc
    38041 tcctggttct acaactacta taaaagcatc tacaccttgg gctgttcctc ttcctaagtg
    38101 ttcaattccc gcttccatat ccataaccac tacatcttta ttttgtagta ttaaatgtga
    38161 gcagagtctc ttcaaaagaa catgctctgg acatacacat ccagtaccac cagaatctac
    38221 ggttcccaat gtaagtaatc ttactccatt gtattcttta cagaaatttt cagggatatc
    38281 gtctacttta ggattcatct taaacatttt tccaaatgaa cctacagaag cagctgttct
    38341 atctgaaact aattttttca tttctgatat aggtactatt gattcatata cttcttttgg
    38401 aaaacctagt gctaaagcta aattagcatc agggtcggca tcaacagcaa caactctgta
    38461 tccgtcttca gcaaacatcc tagagagcat tgaagaaaaa gtcgttttac ctactccacc
    38521 tttacctgtt attgctatct tcataatccc taccaccttt tattactatt tgctttttag
    38581 tagttaattt tacagttaaa tattttttat actctatatt tttatacact aaaaataaac
    38641 tacagctagt ccttacagga accaattaaa tttaccctac tagcttttca aagcagcatg
    38701 tatagtttta ttattcttta atattgttta tattttattt attcagtata ttcattatat
    38761 tttgatgttc tttactttaa tatttgactt tattcaactt tattcaacca gaaacttctt
    38821 ataaaaacgt ttattctata acttcactat tttttaagct tcagcctttt ctgtcatttt
    38881 ttcttcaact aatgcttcaa agtgaacacg ttttttctct atatcttcta ttattgtagc
    38941 tgctaactct tttggatttg tatttacagt aaatgtagct ccaactttat cttttaattc
    39001 actagttaat atatccactg ctcttgaaga accagctatt ggaggcatta ttcctaaata
    39061 agtgtctata ccagaagcta caacatagca tcctatagca actgcttttt ctgacatcca
    39121 ctctggagct atacctacaa ctggaaggtc acacatatcc atatctaagt aattagctgc
    39181 tgctccaact aaatctagta tacggcttat atcaacacaa gaacccatat gtagtactgg
    39241 tgggattcct actaatttac agacagttgc aagaccttta cctgcatatt tttcagccgc
    39301 atcagattcc attaaaccaa acttagctgc tgctgaagct ccacaacctg tagtaactac
    39361 tattatgtcg tttttgatta attctttcat tattgttacg tgagcctcat ttgaaactcc
    39421 tttagcattg ttacatccaa ctacaccagc tgctcctctt aatactccag cttttagaca
    39481 gtctgttaat ggcttaactg ttcctgctgg gtctatatga gagtttacaa ctctgtctaa
    39541 ttgatttact actgcttcta cactataccc aactgttgcc cctgatttta attcaggtat
    39601 aagtactttt gatttatctc tatttttaaa gtttaaaata gcttctttaa ctattgcttt
    39661 agcatcatca agtgcttgtt cttctctaaa ttcaatataa gttgaacctg ttatcttagc
    39721 ttttggagaa gttgttacaa atttagtatg gtaacaatct gcaactctag ctaatgcagg
    39781 gaatatacat tgaacgtcaa ctattacagc ttcaactgct ccagttatta ttgcaagttc
    39841 ttgttgatgg aagtctcctg ctatttttac accatgtctc attgtaatct cattacctgt
    39901 acaacacata cctgctaagt ttattccatc agcaccaact tctttagcta aagctactaa
    39961 gtctggctct tctgatgcta aaactatcat ttctgataat gctggctcat gtccatgtaa
    40021 tattatgtta actttatttt cttctaatac tgctaaattc gcctctgttc ttcttggcac
    40081 tggagttcca aataagatat cgcttagacg agtacctatc atagaaccac cccaaccatc
    40141 agccatagaa gttctaagac tcatgtgtat taaactatct atatcaccag tacatcctat
    40201 atgagtagag tgcattattg ttgcaatttc tctatctata gctcttggct ctatagcgta
    40261 ttctttccat attttttgtc ttggttctgg tgcatttttt ataaatctta atgtcccaaa
    40321 aggttttcca aattccatta atgcaatttc tgccacttca tgtgctacat catagatatc
    40381 tctaccttct gtctctacat cccattcttt agctaaagta attaacttag cttcgtctct
    40441 tattttatag tttccatttg gatcagctaa tgctaatgta tgtgctatat ctctagcatg
    40501 gtctgaatgg gcagctgtcc caccagcaac cattcttgca aagtttctac ctactatagt
    40561 atgctcatct gctccacata acccccttgg agtcttaggg cttattctac atggtcccat
    40621 tccacatatt ctacagcaaa caccttgaag accaaaacca cattgtgcct taaagccttc
    40681 ttttctatca tacattgttt ctacgccttc ttctctagct ttagctatca tcttctggct
    40741 gtttaaatca attgttaaca ttttttcatc catattccta tccccttttt ttgattttta
    40801 aaatccccca ggtgactgac ttcatgtcat aaaataataa aataaatgct ttctaagttg
    40861 attatatact aattttcagt gctttgcttt agatttaaat atttttttaa caaaattaga
    40921 taatacataa aaattttatt ttctacactt aatttaagtt gattttatcg cttgtttttg
    40981 gctatttgtt aatatctaaa cctacttttt tgatacttca gtttaattaa attatgtttt
    41041 gattttttta aatcaaattt gaaatttttc tagtcatttt tatattattg cagattgttt
    41101 gtgtatttat tatctttcac tactgtttgg acttattaca gatttttact ccctaaactt
    41161 aattagtaaa aacttcttag aatagcattt tttggactaa tttttttgaa tttttttatt
    41221 tttcctactt taatattaat tatagcttca aattgaacat ttgtatatat tcatttgaag
    41281 ctataattat tttttatata aaactttttt aatacagcat taatttagac attttgacta
    41341 ttttttctac tttaattcat ttatttcaag cgatttatcc tatttcatat ttttagctac
    41401 aatagcccca tatgcaatag aatataattt agattcatgt gaatcagctc ttgacactaa
    41461 aactatagga gcttttgcac ccataattat acctgctgat ttagagtctg ccatatatgt
    41521 aaaggctttc cctattccat taccaacctc tattgttgga accaatatga tatctacatc
    41581 tccagatact tcacttttaa acccttttat ctcactagct tcttttgaaa cagctaaatc
    41641 aaaagccaga ggaccttcta caattacatt ttcaccaaat tctccttctt tacaagcttt
    41701 tgctaacata tctgcatcta cagttgcttg catcttagga tttactttct ctttagctgc
    41761 caaacatgca acttttacag tttccattcc taaagctttt gcagctttta tagaattttt
    41821 taatatcttt gccttttgtt cataatcagg agctatattc atacctccat ctgtagttat
    41881 aagtaattta tggtattttt ctaactcata taccataaca tgacttaata aactatcagt
    41941 tctaagacca tagtctttat ttaacacttc ttttaatatg acagatgtat ctaataatcc
    42001 tttcattaca aagtcagctt ctttacttga tactaattta actgctattt ctgcacactc
    42061 tttgatatca tccttatcca ctattttgat accacttaaa tcaaatccta tttcttttga
    42121 tatttctctt attttgcctt catttccaac caatatagga gtaattatat ttctttctac
    42181 agcatccttt attgccatta aaacttcttt atcatgagct gctgctacag agagtacaat
    42241 tttctctcct cccttaagtt gttctaaaat atcatctaat ttttttatca aaagtaaccc
    42301 ctcccttata tatatttaac taaataatac catatttgct ataccttttc tcaatttatt
    42361 ttttatgtta taaagaattt atataactaa aagacagttt aatattttat tctgggattt
    42421 catcaataac tcttaaaagc tttgcaggaa caccacctac aatagtattt tctgcaacat
    42481 tttttgttac aactgctcca gcagcgataa ttacattatc tccaatagta actcctggaa
    42541 ctatagttac attagcacca atccatacat tttttcctat tgtgattgga gaaggataag
    42601 ttgtaccacg tttctctggt gcaataccat ggttaagtgt agccaatacc acattatgcc
    42661 caatttgcgt attatctcca atatcaatcc ccccctggtc ttgaaaatgg caactgctat
    42721 ttataaaaac attttttcct attgtgatgt tttttccaca atctgtataa aatggtggaa
    42781 acataaaaaa tgattcatct accttttttc caattaactc agaaaataaa ctacggactt
    42841 cttctggttc atgataagaa gaatttaact tacttgtgat tttcattgct tcattactca
    42901 acttaaccat aaattcatgt atttctgatt tactagaaac aggttttctt tgattgatat
    42961 gtgttaaaaa ttcattgata ttcattttat acatcctttc aatatgtgct ttaatttatt
    43021 tgtacattct aaataatatt ttaataaaaa ttaatttaat ttatttttat taaaatatca
    43081 ttgcttcttt tagtgtaata tacaattata tctatatcaa atatctatat taaatactta
    43141 actatactta aaaagcatat aaattgatat aattatataa tagataagtt aatattgagg
    43201 agggataatt atggaaataa gagtattaag atatttttta gcaattgcaa gagaagaaag
    43261 tatatctggt gcggcaacct ttttacatat aacacaacca actctttcta gacaaattat
    43321 ggaacttgaa gaagagttgg gagttaaatt attggttcga ggaaatagaa aaatcacctt
    43381 aacaaatgaa ggtaggcttt tacataaaag agcagaagaa attatatcat tagttgataa
    43441 ggttgaatta gaatttgaca atcccaatga gattattagc ggtgatatat atataggaag
    43501 tggtgaaact gaagctatgc gacttatagc tagaactgcc catcattttc atcaaaaata
    43561 ccccaatgta agatatcacc tttttagtgg gaatgctgat gatgtgactg aaaaattaga
    43621 caagggactg cttgattttg gtattttaat tgaacctgca aatatacaga agtatgatta
    43681 tgcacaaatt cctgctactg atgtatgggg gttactaatg cgtaaagata gtgcccttgc
    43741 atcacaaagc acaataaatc caaaagattt atgcaatata cctcttatta tttcaagaca
    43801 gacacttgtt gataaagcaa tttctaaatg gcttcaagac agtaatgaaa aactaaacat
    43861 tattgcaaca tacaatttag tttataacgc ttctcttttg gtagaagaag gtctgggcta
    43921 cgctctgtgt ttagatagac tcgtcaatac tacagaaaac agtaatcttt gttttagacc
    43981 tctatctcct cctttagaag ctaaattaaa tattatatgg aaaaaacatc aaatattttc
    44041 aaaggcagca gaaaaatttt tacttcaaat acaaaaagat ttttcttcta tcatgttgga
    44101 tacatctaag ttggatacac ctaaagaaaa ataaatcata ataaaaagtg agagtgtctc
    44161 aaaatgaact ttgcactgta taaggcactc ttttttatac aaagtaaaat atatgttcat
    44221 catttcagca ctaaaaaaga ggcttatctc tattttgaga tagccccact taaccaataa
    44281 aattaaatac aacttaacaa tatccttact tatttattcc aagttctctt agacaagcaa
    44341 tagtaatttc atcttgtgga tttaattcat aagctctttc tatatagtaa gttgcatcat
    44401 caatatcacc attatttaaa taagccattc ctaaattgca aagtatttct gggtcttcct
    44461 ttatcttagc cgcttgttca aaatactcta ttgctttatc taaattattc ttataagcct
    44521 cacaaagccc caattccact atagtatcca cttgattagg ctttattatc aatatcttct
    44581 caagatacat cttagcctgc tctatttcgc ccataccctt atatccaagt gctatcataa
    44641 acagcaagtt ccaccaatcc atatgttctt cttctaatgg aagtaacttc tctaatcctt
    44701 ccttaaactt accttggaat acaagtgtat atccttcttc atattgaact ttaaagtcca
    44761 tcttgcccag attctcttgt acctcagcta ctaaatcagc atctaaccct agccttaacg
    44821 cttcttccca agtcagctta gtctttaaat attgaccttg attataataa tggtagccta
    44881 agtgataata acctaatgca aaattctcat caacatcaat taccttttca agcttagcca
    44941 tggcttcaag taagaatgca ttcattgcct tatcatccat atctttttga tactgtttag
    45001 ctatctcttg gcaaacaatc gcatagttat acattgcatc taaatcatct ggatacattg
    45061 ttataaatgc ttttatatat attaaagaat ctgtatgttc accaatctca aagtattttc
    45121 tcgacatata tcccatatac gatttcaaat ccaaatcaat atcttttgat aaagcattta
    45181 aaaacttctc atactctgca ttgtttttaa acttactatc tattccaatt atatatatca
    45241 ttgcatctgc tatagacatt gaatttaact tatcttgagc agtcttttct tttatccctt
    45301 ttactaatac ctcatttttt ataggtacat ctaatccacc ttttggcact tcatagcttt
    45361 ttaatttaaa ctctccacca tgttttatag ttataaaagc taattcttct gtttttttta
    45421 gcaaatattt ttctatctta aaatccattt tatcctccta aatacttaaa tctctacttt
    45481 atgagtttta gatttctcaa atattttttt atcatcatac tcaaataaga ctacattttc
    45541 tacttctctt atttctgtat ttatatcatt ttttaaatcc acaacattaa atctaaatgg
    45601 ctcaaaatga acatatttta ttatatgttt agctggttta tcaacaaaac taggtaaata
    45661 tgttgataag ttatcattat cttgtaaaaa tacatggcat ctattcttaa aatcatccac
    45721 atctagctta ttaaaaaact gtggtatatt tgatgtttta cccagtgata tatagtcata
    45781 ttttaatata tcgttgaata catcattatc tatgttgatt ttttcattat agaaatccat
    45841 caatatttta tataactgat tctttccttg tgccaaatca aagtagccat tttcatcaaa
    45901 atacttggca aaaacctcaa agaatttaaa agggcttttt ttataaaatc tgcctataat
    45961 atatctcata gataacacaa agtttttaga attgtagtat ctctccagta tatcttctat
    46021 atcctttagc ttcaaagttt cttcatagct tatataatta ttgtataaaa actcatatgg
    46081 agcataatcc ttatatttaa aaccatgctc atctgcaact tttctcatac ctgtcccttt
    46141 aatcatcttt aaaaagccta attgcaaatg ctctatacct agattaaata catcattaaa
    46201 agaattttca aagctcttat aatcttcata aggcagtcct gcaattaagt ctaagtgctg
    46261 atgtatattt ctataagatg ctacagtttg tacaacatga gacaactttt taaaatcatc
    46321 ccttctacct actgcatcaa gtgttttttc attcgttgac tgaacaccta tttcaaattg
    46381 aaaaagacct tctttacaat cagccaaaaa ctcaagcatc ttatcatcta tcaaatacgc
    46441 tgtaacttca aaatgataag ttgtatatcc attatcattt ttcataagga aatccatgat
    46501 ttccatagcc acttttctat ttgcattgaa tgtcctatct ataaacttta tttgtgatac
    46561 tcttgcatct attaaagctt ttaaatcact ctttatcctg tctaaactaa aataccttag
    46621 acctggaata gtagatgata aacagtattg gcagttaaaa gggcaacctc ttgatgattc
    46681 ataatacact attctatttt catattcttt tggattcaag ttttcataag gactaggaat
    46741 ctcatccaaa ttttgaagta attccatagg cttattcact attatctcat tgccttgtct
    46801 atatacaagt ccatttacat catttatttc catctttcct tgcaagtgaa gtactaagtc
    46861 tctaaaaatt aattctccct ctccataaag tatataatcc acaaaatcat actttttcat
    46921 tgcctcataa ctatcataac tcacttccgg tccgcccaaa gctattttag tattaggtct
    46981 aatttttttt agattatcac aaagtttaac tatatctccc acattccaga tatatgttga
    47041 gaataatatt atatcatatt catttttata tatatccttt aaaatatagt ccaaatcgtt
    47101 gtttatcgtg tattctttca tattaacttc tatcaaatct cttacaaatt cttttagata
    47161 tcttatagcc aaatttgtat gaataaactt ggaatttaat gttgttaata aaattttcaa
    47221 atatttctca cctcttttta agattatact gtctttttgt accccttata ggttaaaata
    47281 gtatatgtaa agtctaaata atatctatat aattaattta ttaatttcaa gtaatgtgag
    47341 gaaaggaagt tttttatgga attaattgaa aaagtatcta tcaactcttt atcaaaaaga
    47401 gatttactac ttattataaa gtctcttgaa tacactaatg aaaatactca aattagtgat
    47461 ttcattgacc ttaggaataa tatagtaaag gaattatgtt ttttaactga cactaaggaa
    47521 aaagattttt tagattatct agaaaaaaat agcacactct aataacctaa acctttttaa
    47581 actttaatat ataaatacct gatttagaat ttatttttat gcttaatata ttatcattaa
    47641 aggttttcga cataactgtt atttgattaa atatagggtc tattatagta ttaccttctt
    47701 caagtcttat aagacttctt gggtctatgc ttttattata tgccttaact ccaattttat
    47761 attttctcaa tttaacaaca tattcggaca tagaaaaaat aaaatctata ttatcttcat
    47821 ttaatttact atcttcaccc aaaatggata atatagattt ttttatgcta ttaattcctt
    47881 cattttctac aattctatct tccatatata catcatatag aacatttaaa acatttaata
    47941 aaatactttc ttcatcataa tcttcagatt tcaaatcaat gtacttatca attaattgta
    48001 ttgctttaat ccttgccatc tgaaacttta caacatctga tttgtctaat tctatagaaa
    48061 atcctattat cttgtcttta atactctgta gtaattcgac atactctatt ttactattat
    48121 ttataagatt gtgcataatg gaattataca ccacagaacc taataaataa tcaaataaaa
    48181 gttcttcttt tttaaaataa ataacatact tttttattag tttaataacc tcatcttcac
    48241 ttttttgaat attagtattt gataaaatta gtggtaataa aatatttaaa aaattgatat
    48301 ttcctatttt ttctccataa aatctatagt aaataagttc atctctcagt ctattttcgt
    48361 ctatatacac acatctttgt gttttatcat ctattaagga ttttgtattt attatagtta
    48421 aatctttaag agccctactt gcactactaa aagaagatgt gtagactttt tttagtaatt
    48481 cgtcattcat aaatatatcc tttctgaaaa attgaatgta aataaattgt tattaaaaaa
    48541 aaaagagaca gcccgtttaa gaagctgtca tatatagggg gatagtagtg tacaaaaatc
    48601 attgtaatct actatctata tattattata cgatactact tttttatatg taaagcatca
    48661 gttttcgaca tccctatatt ttacagttat acaaattgcg tactaagttc aattttattc
    48721 tttttatttc aacattttat catgctacat tctttcagga gcgtgcattc ccaacaattt
    48781 cagagaattt tctaaagttt gttttgttgc taaaactaat gcaagtcttg cttttcttat
    48841 ttccaaattt tctactaaaa ttggattgtc atgataaaat ttattaaacg cttgagctaa
    48901 atctagcata aatctagtca ctatatgtgg ctcatttttc tttagagcaa gtaaaatatt
    48961 cttattgaat gattctataa ctctaagaac ttctgcacta tcttcatcag ctaatagact
    49021 ataatctata tcagaagtca cttctacttc tgcttttctt aaaactgcac agcatctagc
    49081 atgtgtatac tgaacatatg gtccagtctc accatcaaaa ctaagcgttc tttcccaaga
    49141 gaaggtatag tctttaattc tactattaga taactcttgg aatactactg ccccaacacc
    49201 tacttgctta gatatttcat ccacattttt agcatttggg tttttagcta gtataatttc
    49261 cttagtttta tctatagctt gatttaaagc atcttctaaa aatactactc ttccttttct
    49321 agtcgacata gtaccttctt caagtgcaac cataccaaat ccaacatgaa ttaaatcttt
    49381 tgcccaatca tatcccataa gctcaattac cttaaaccat tgttggaaat gtaagttttg
    49441 ttgagaacca actacatata tacacttgtc aaaatcataa gtttcttttc tatatattgc
    49501 agcagctaaa tctcttgtca tatataacgt tgaaccatca ttcttagtaa taagtgctgg
    49561 tggcatttta tactcttcta aatcaacaat tcttgctcct ttagactctt ttaaaagatt
    49621 tttctcttct aatagttcga taaccctgtt cattttatca gaataaaaac tctctcctgc
    49681 taaagaatca aattctatat ctaaaagttt ataaactctg ttaaactctt ttaaactctc
    49741 atttctaaac cattgccata attcttgtgc ttcttcgtca ccattttcta atttattaaa
    49801 ccatgctctt gcttcatctt ccatttctgg gtgttgttca gcttcatcat ggaatcttac
    49861 gtataatttt agcaattctg gaattggatt agactctact acttcttttt ctccccactt
    49921 tttaaaggcc actattaact taccaaactg agttccatag tcacctaaat gatttattct
    49981 tatagtcttg tatccttgcg aatcatatat cttatataaa gcattaccta taactgtagt
    50041 tcttatgtgc cctatatgga aaggttttgc tatatttgga gaagaatatt caactattac
    50101 agttttatct tttccaaaat cactatgtcc gtaattttct ttttcatcta aaactttttt
    50161 aattacagtt tctgctaatt gagatttatt tacaaagaaa tttacatatc cgcctaattg
    50221 tattactttt gttatctctc cacttggttc tatactttcc gccaattcac ttgctatcat
    50281 gtttggagct tttctaaaca ctttggctaa tttaaagcat ggaaaagcat agtcccccat
    50341 atccttattt ggtggaactt ctataagtgc ctctatctct tctttcgata aatcttctat
    50401 tttttcttta aggcaatttg atattgccac cttaaaatct tgcattctgt tgctcctctc
    50461 tacattgttc ctggtttata ttgtgcttat ctctttatgt taaatcaaat ttcgtgtttt
    50521 ttcaagtatt tcattacagt gtaaaacact ttagtaatta tgttttctgt atatattaaa
    50581 aatactataa ttgcagtttt taatcacact attattgatt ttttataaga cttttttcta
    50641 ttttattgtt tcttgtacta tccagttgac tttatatact ttttttaatc ttttatctat
    50701 tttcatacgt caatgctaat tttatatact tttcttttaa tcttttatta attttcatac
    50761 atcaatgtta actttatata cgttttttta atcttttatc tattttcata catcaatgtc
    50821 agctttatat acttttttta ctcttttatc tattttctat aaatcaatat tagctttata
    50881 tacttttttt actcttttat ctattttcta taaatctttt tcactcttaa cttttattcc
    50941 ttcttcttct aatagccttg ctgttatacc tttccctggt atctttttac ctgaaaaact
    51001 accatcatat attagattac atccacaaga ggggctaccc tcttttagta ttgcttcgct
    51061 acatacatat aaatttgcta tttttagagt ttcattagcc cctcttacaa agttttctgt
    51121 tacatcctta ttatctttag taattacttt tccatcaact atctcagcag gtggtcttgg
    51181 tgtagataga ccacctaact gctctgggca tacaagtgta aattctttgc cttttaaata
    51241 ttccttgact ttttcattat cattattatc gccactatat ttacaattta ttcctattaa
    51301 acatgcagat accaatatca taaaaatgtg ctcctataca aattatatat attctcatac
    51361 aaattactaa tgtattctta tacaaattat tttaatttaa caattgttac tcctgctcca
    51421 ccttctccat attgaccacc tctttgtgat tttacatgtc tatttcttct taaaatgtct
    51481 tgtagccctg cttttaatac tcctgtacct attccatgta ttactgttac agactctaaa
    51541 cctgccacat atgcatcatc aagatacttt tctacttcca ttatagcttc ttctaggttt
    51601 aaccctctaa ggtcaacttc attttttaca ctaccagatt ttgaccttat aatgtttctc
    51661 gttgatttag ttacatttgt tgaaacatct ttgcgtgttt tttgtaaaga tttaaaaggt
    51721 agagtcatct tcattatacc aatttgcact actgcttctt ttctcttttt atctactgaa
    51781 actacactac cattttgatt taaagttatt accttaactt cttcacctgg ctttaagtct
    51841 tttatctcct tatttgatac ctttggaact atcatacttt ttactgttgg ctgaagactt
    51901 cccatagagc tagtcaattc ttttctaagt tcttctatct tacgattctt ttcttttgaa
    51961 gctctttctt gttcaagact tctaagttct tttattataa tatctacttc ttctttagct
    52021 tgtcttataa ttgaaaatgc ttctgattta gctttttcaa tcatcttatc tctttgactg
    52081 accaattttt caagtttttc gtcatactct actttcagtt tttcaatctc ttcttttaat
    52141 ctttcagctt cttctctatc ttctactgct ttgattctat tcttttcaac attctgtaac
    52201 acatcttcta aagctatgtt ttctgtattt atatattctt tagcacgtga aatcacataa
    52261 tcacttagcc caagttttct agaaatctca aacgcatttg attttccggg aacacctata
    52321 agtaacttat atgttgggct taaagtttct atatcaaatt caactgctgc attttctaca
    52381 cctgatttag taagagcata atttttaagc tcactatagt gagtggtagc tatacattta
    52441 gctcctacag aatttatatc ttctaatact gctattgcaa gggcagctcc ttcaacaggg
    52501 tctgttcccg ctcctaactc gtcaaatata actaaagagt ctgctgttac gttttgtaat
    52561 atagatacta tattcgtcat atgagatgaa aatgttgata gactctgttc aatactctgc
    52621 tcatctccta tatctgcaaa tacattatca tatacacaca tactacttcc ataatcagca
    52681 ggtatatgaa gtccactttg tgtcataagt gcaaatagac ctacagtttt tattgtaaca
    52741 gtcttaccac ctgtgtttgg acctgttatc accaatgtat ggaagtctct tcctaaataa
    52801 attgtatttg ctactacctt ctttttatcc agcagtggat gtcttccatt ttttatgttt
    52861 aaatatttat cctcatttaa agttggttct attcctctca tctgtataga aagttttccc
    52921 tttgaaaatg caaagtccaa tcttccaagt atctcttgat tagaaattaa gtcttcacta
    52981 acttctccaa ccatagcaga aagctctgat aaaattcttt ctatttcctc ttgctctcct
    53041 agtttcaact gcctcaattc attgttcatc tctactatag tcattggctc tataaaaagt
    53101 gtcgcaccag aagatgactg gtcatgcact ataccagcta cttgcgacct atattcagat
    53161 tttacaggta ctacaaatct atcacctctt agagaaatta ttgcatcttg taagtacttt
    53221 tgataagtag ttgaacttat tattgaattt aattttgacc taatggattg atttttttgg
    53281 gctattcttc ttctgatatc tctaagtgta cttgaagcat tgtcagatat ttctacttca
    53341 ctaattatag cattgtatat ctggtcttct atgtccttgt atacatacag agaatttgat
    53401 agagattgta ttattggata attaaaatct tcttcatcac tactagataa gctatttctt
    53461 aatcttctag ccactcttaa ggtatctgct atcataatca gacttcctgg gtcaagagaa
    53521 gcacctatat aagctctctt taccttatct tctatatcat gaataccttc caaccctact
    53581 gaacctctct ttataataat tgattgagct tcactagttt cttctagcat agatttcact
    53641 tctacaaaat cagtatttgg tactaaattt tctatatatt ttagacctaa agaagatgag
    53701 gctttcttct ttaacaaatc tattatttta ttgtattcta gaactcttaa cgatttctca
    53761 ttcattttct atattcacca tcctcgatat tttaactttt ttatttaatt ttaaaaatgt
    53821 atgttttcaa gcatttccaa taaaaactgc aagttttatt tctttttcat ttctaaaatt
    53881 attatatttt ttctctttta gatatatttt acacatattt tgtattatac tatgtatgta
    53941 gctatctata tattatacat aaacatgctt tatttgtaat ataatctgtt aaaaaatctt
    54001 ctttaatatt tttaaaggag tttattttag cttataaaaa aaaatagcta cttatataga
    54061 taaattaagg aggaacatga gtcatgggaa acgtattaga aggattaaaa ccagaaagtg
    54121 tttttaaaaa ctttgagaaa atatctcaaa ttcctagagg ttctggaaat gaaaaaggca
    54181 taagtgattt tttacttagc tttggtaaaa atttaggtct tgaaactatt caggatgaaa
    54241 gcttaaacat aataataaga aagcctgcta caaaaggata tgaaaattgt ccaggtgtag
    54301 ttcttcaagg acatatggat atggtttgcg aaaaagaaaa gaatgttgaa catgattttc
    54361 ttaaagaccc tataaaatta agaattgatg gtgacatgat atatgctact ggaactactc
    54421 taggagctga caatggtata gctgttgcta tgggtatggc tatattagaa gataatactt
    54481 tagagcatcc tgcactagaa gttttagtta ctgttaatga agaagatggt atgaatggtg
    54541 cagatgcact agaccctagc ttaattaaag gtcaatatat cctaaatatg gactccgagg
    54601 aggaaggata cttattagtt agctgtgctg gtggtaaaac ttgtgttgtt agtttacctg
    54661 ttgaatacaa ggaagttaaa ggtgacaaac aaggattatt agttgaggtt actggactat
    54721 taggtggaca ctctggtatg gaaattgttt tacaaagagc aaatgctaac aaagcaatag
    54781 ctagagtatt aagtgtattg aatgttgatt atgaattagc ttctgttgat ggtggtacta
    54841 agcacaatgc tatacctcgt gaagctaaat gtgtaatagc tgttaataaa gctgatgttg
    54901 aatctgctaa aaaacaaata aatgacattt taactgcatt taaacatgag tttactactt
    54961 ctgaccctgg catgacttac tcagtagctg aaacttctgt agacaaagtt cttactaaag
    55021 attgtaaaga aaaagttgtt caaatgtctt gcttaactcc tcatggagtt caatcagtaa
    55081 gtcttgatat agaaggatta gttgaaagtt ctactaactt cgctataatt gaaactaaag
    55141 aatctactat tgaattttta acttctgtta gaagttctgt tatgtctatt agagatgaaa
    55201 tcgctgatag aataagactt ttggctcaag ctttaggtgc taattacgac cttatagctc
    55261 aatacccagc ttgggaattt aaaaaaggct caaaattaga aaaaatatgc agtgaaactt
    55321 atgagaaatt aactggtaaa gttcctactg ttatggctct acatgctggt cttgagtgtg
    55381 gattattact tgataaactt cctcatgctg aagctatctc tataggacca gatatgtttg
    55441 atgttcatac tccaaatgaa catgttagta tcccttctgt agctaatgtt tgggattatg
    55501 ttattgaaat attaaaatct atgaaccaat attaaattta agttttgatt ctaagtagat
    55561 tttaatttta agtaatctat ataaaattaa ctctgtaaat taatactact gttagtataa
    55621 tatttacttt caatattaac ttatagaaat aaagcctaat gatagataat aacatgtatc
    55681 attaggcttt attgtaattt aaatattatt aagctttatt ctgttttaaa attaattctt
    55741 atactatttt aaatctattt cttccttgtc tcttagaatc ataaagagca gtatctgcta
    55801 tttgatacag ttcatcatac tcttgaccag gctttgctat aactaccccc atactaatag
    55861 ataaactttc tccatttggc aacttaatga gatgaatatg attgtataca tcattacatt
    55921 tattaataag ttcttcctta tttattatat cattgatata aacactaaat tcatctccac
    55981 ccaaacgacc tactatatca ttttttgcaa aactttcatc aagtaccttt gcaacatcaa
    56041 taagtacttt atctcctaac aagtgaccat aagtatcatt aatcgactta aaataatcaa
    56101 tatctataat caataaagca ccattttttt cagttgataa attcaacaaa ttttgtgcag
    56161 ttaattttct aatatatgct gcattataaa tattggttaa actatccctt tgagccttat
    56221 ctaacaatct attcttttcc tctttttcag aatcaatatt acatattttc ccgattacat
    56281 acattggtat ttttctatca ttataaatta tttttgaagt aactctaatc caatgaaaag
    56341 tatcatcaag cataatagaa tacatttcag ttgttccatt ttcttgtgat tttattacat
    56401 caaaaaactt tttattatat tctttcctta ttttatctct ttcattttct atattgccat
    56461 tttcactttc cttatcattt ttatccaaac cataataagt ctcaataccc ttttctggca
    56521 tagaaattat caatttatct tgcttataat catactcaaa aaagtattca cttgtcattt
    56581 catacaactg agtatgtttt ttgagttcca aagatatctt ctcgttcata cttgtacgag
    56641 tatgtaaacc aaatgccata aatccaataa tacaaattaa taaactggct acaataaaaa
    56701 tagtctcaat tggattctgt tgcataatat aaagcaacga gaaatcttgt ctatatgtag
    56761 tattttggta gacaattgat tgcatttttt ctgatgaaat acttataata gctttattaa
    56821 atatattcaa taattcttgt ttatcttttt taattatacc aaaacaaata ctctttgctt
    56881 tataggtttg aggaactaga cgcacatttt tatacttagg ttgattcata tagtactgca
    56941 tagaataacc ttcgccataa gtgtaatctg catagccttt attaacagct tctatacact
    57001 cttctaaatt atcataccaa actatattct cttcaaactg accataataa ttaatacctt
    57061 tggctagagc tacacgtttt ccatctaaac tattctcatt taatttatta ttaatcatca
    57121 tagtatattg agtagaaaga aaaggccgag acatagctat attatgttca cgagagtatt
    57181 cataatcata aatcattcct gctacaatat caatttcacg ctttttagtc atgttttcta
    57241 aatcttcttg agattttaca ggcacaagtt caaattctaa gcctgtttta tcacatatat
    57301 gatttaacaa atcaattgat atacctctta attcacctgt atctttatct atattttgaa
    57361 atggtggttt gtttaaaagt actcctactt ttaatttttc tgtctcttta atgtaattag
    57421 tttcttcatc agataaaaac atttgattat tctctggtgt aaagtatttt tcatataaag
    57481 tagttgaaaa atatgggtct ttctgttcaa tattattaat tgctgagtta agcttttgag
    57541 ttaaatttgt actaccttta ggagtaatga aataaaatgg ttttgatgaa aattttgcta
    57601 cagtacgtac accttctatt gggttcatac tcacattaag aagtacatct gctttaccac
    57661 tctttagtgc ttctagttgc tcatcatttg ttttacaata taccaattca ggtgacataa
    57721 gattcatttt acaatactca tctaattctt taagtctagt atcagaattt tctattacag
    57781 caatacggat ttttttcaca tcttgtgaac tcattatgtt atcaatatct gtattttcat
    57841 ataatgcttg tagtactgta tttgctactc cataattatt ccctgcataa ttatatattt
    57901 tttgcattgg ttcattgtat aacatacctc ccattatatc aatttcacca ttttctaaca
    57961 tcttcattaa ttttgtaata gattcattta tctctccagg aacttgaaca aattcataat
    58021 tccatccagt atactgtgca atttctagta aatattcata tgtataccca aaatagttac
    58081 cttgctcatc tatttcagtt aaaccttttt gaataggata agcaacacga atggtacgat
    58141 tatcattacc tatagcgttg ataccaaaag aaaatatgca agaagtgaat atagatgtaa
    58201 ttacaataat taataaaagt aattttttta aattcactac acaatcctcc atatagttaa
    58261 gatttacagt ataaattact gcataaactt gtagacatgt aaaaatagat tagtatataa
    58321 aaggatatat aaatctactt agaaatttaa atatccttaa tatatacaat atatttaaat
    58381 tataactcat ttatatgtct attacaacaa atttaccctg ctaaatacct actctatatc
    58441 ccataatttt acaagttgta gtgaagttta agttctatct cacctcaaat ttagcagtgt
    58501 ttctatttac agttctttta tatttcacat ctggataacc caatataaat gaagtgataa
    58561 cagcttcgtt ctcgctcatt cctaacagtt cttttatctt tggctctaca tttgatgcca
    58621 taacaaaaaa tccattataa caaattccta atccttgaga ataggccata agttccatat
    58681 tagatgcagc caatcctcca tctacataag cacttccacc tagagacata tcacctacaa
    58741 ctaccataag tagtggggca tcataaaata aagaatcatt tccgttctct ttgtatcttt
    58801 tgtacatttt cttaaatgta tttttataca tagaacgtac agggtcattg tcatcagtat
    58861 ctaatgccaa attataaaga ccttgtattg ccagttcttt cacttctttt agcttttctt
    58921 ttactaatat gtatcttata ggctgtctat taccacctgt tggtgtgtat ctaccagctt
    58981 ccactatgtt ttttattttt tcttcttcta cctctatatc tttatattgt cttatacttc
    59041 ttctaaattt aataaaattc aataactttt cgggttctaa ttcaaaactt tcacgattgt
    59101 attctattac ttcattttta tcaaatcctt gcatttctat tgcatctgtt gggcaaattg
    59161 ccatacagtg accacagtta atacagagaa tatttttaac ctttgcttta tcatccttaa
    59221 cttttattgc atttacaaga cagtctgatt cacacatacc acagcctaca catttttcta
    59281 aatttatgtc tatcattcca aattattcct ccttttaaaa aatttacttt agtataaaac
    59341 aaatagttgt ctaagtcaat ttttattttt ttaacaatat actagcctta actacacttt
    59401 aatggagaaa aatatacata aattggaact taaattagaa cttattcaaa gtaaaagata
    59461 aaagaagtag atgtctaaaa gtgaactttt tagttaatta gatattcttt attgtatgaa
    59521 atcaagtgga tatttatcat ttcaacaata aaaaaggctt acctctattt tggatatgtc
    59581 cctgtcaagt agacagacta aaaaaaggct atctttatgc actgagatag ccccttttcc
    59641 caactactat tgtttatttt aatattatat caataaattt aatttgctat aacctgtaag
    59701 ttacatttta atttgatgta actttaaatt acatttttaa gtagtatctt taaattatat
    59761 tcttaaaata tatcattaaa ctacatttta attaatctaa aaactatatc ttaattaatc
    59821 taagaaactt acattataat cttttagttt cccattatca tatacaatct ttgcactaaa
    59881 gatatttcca gtcttagccc aatttaagaa cagcctgtca ccttcccaca tatttaaatc
    59941 gaatatttta tcattatcaa cccaaattaa atttccttct ttacaatcat ttaaatcttt
    60001 ttcagataat tcaccttcaa actcatctga gaaatacaaa aacatatacc aatcttcttc
    60061 atctttaaat tttggaaaag ttatcaaccc tttatatgac aatgatttaa gagtaagacc
    60121 agtctcttct tttacttctc taattacaca ttcttctgga gtctctcctt gctcaaactt
    60181 tcctccaact cctacatact taccttcatg tatatcatct ttctttttat ttctgtacag
    60241 catcaaagtc ttgtcatctt tttctatata acaaatagtt gtaagtttca tgtatctcgc
    60301 cttgctttct cttttatatt ctcaactaat tattttcagc actttcaagc acatcactta
    60361 tattcttcat tgcattaaga gaaagctctg caaattcatc aaatggaatc cctatatctt
    60421 ctatacttct cattgcatct ctatttacat ttgaagcaaa tctcgtttct ttcattcttt
    60481 taataacaga cttagtcttt acactggtaa gttttttatc tggataaact aaagctatag
    60541 cagttataaa cccagttata gggtctgctg catatattgc tttttccatt aggctagttc
    60601 ttgtaactcc cattccatca tgatgaccaa gtattgctct atacatttct tcgtcaccga
    60661 agttttcttt tttaagcagt tctactgttg ttgtagcatg tccttcacat atttctcctg
    60721 gctttcttcc aactatatct gaatccaaat catgtaaaag acctgccact gcccactctt
    60781 cttctttttc tggctctagt cttttggcta attctctcat aacagcttca acagcataac
    60841 aatgttttct cattctatca gtttctaaat acttaaataa aatttctaaa gcttcttctc
    60901 tcattttcac caatccccct gttttttcac atatatgata tttttatata ttaatacaca
    60961 taaattcaag tttttatata tattcatgta tatttttcat gttatatcta ttctacacct
    61021 ttatagtagt gtcctgcaac tgttccaatt tcatctaatt tcttatacaa gtctgtagcc
    61081 ttttttccaa gtttataatt attactcaat acctcaatgt gagcttcaat aaccttttta
    61141 accatctcac tatcttcaac tgtaaaatct atcctaaata catctattcc aatttcctct
    61201 aactcatata gattgtccaa catacaagta accttcgaat tgtaaattgt acttctacag
    61261 aatatatctt gtgttatcct ataacttgca tccgtaaagt ctcttaaagc ataactactt
    61321 tccttacatt tagcaactct cttgtccttt ttacaatctc taacaactac tcccatagga
    61381 cagtattctg taaccatcat aggagcatat ccatatatta ctgcttcaat ttctacatct
    61441 gtatatttta aagtttgttt aatttcattt aagtttagtt cttgtgacaa acaaactgta
    61501 cttgctccct cttctttgaa gtgatttata ctttcactat tgaatgcatt taagtagcaa
    61561 tctatattta agtctttttt cttaaagtat tccatagagc cccagttacc aatttgaacc
    61621 ttattaattc cttttatatt atccaatatc tcaaattgct tgtactcttt atttctaaca
    61681 atccttggtg ctgaatagat taattttttg tcagtgactg ctgtattata gtggttctta
    61741 ttcaaagtat ctattttact tgtatttgac aaagtatcta tgttatttgt atttgaaccc
    61801 attagttctt taggagtacc taaagacgaa gaagtatcta aatccataga aatacttgaa
    61861 tctatagaaa tatttgaatc tatagaaata cttgagacca tttgtaaagc ttttgataat
    61921 gtatttatat cttcataata gattaaatct acattataat ttaatactgc ttctaattgc
    61981 tccaagttct taactttaac tctaatctta ggagctgtat caagtttttt ctgtttattt
    62041 actctatttg gctggtattt taatctattc ttcttaaatt ttctatcttt tatctttatt
    62101 ctttcttcat ctaaaagctc aatacaatcc cttctcatct gatttaacac acttataggc
    62161 atacttacac cttcatccag gtcaatttct atctctttta acttatatgg tgtatttcca
    62221 agcttttgaa tttgtgtttc tattttttct ttacttaaag ccacctttat agctttttca
    62281 actattttgt ctccttctac caccgattta tttccacaaa aatcacttat aaaaagctct
    62341 ggctttttac ctagtttaat actcactttt gcacgtatat tagtttttat gaactcttta
    62401 tcttgctgaa atgtagcttg tactctatcc attaattcgc tgtcagaagt cttatatata
    62461 agctgatttc tctttgcttc tccaacaaaa tctaattcta tcaaatcccc tttatgtgct
    62521 attgtagtta tctctccatc ctttattatt cttccaatag tacctccacc taagttaatt
    62581 ccatcacctt tcttaagcgt attttctagc attactctaa gtcttttgtt ctttctatta
    62641 taatctaaaa cttttccaat atatagccct tgattattag gtctattaga gttcattata
    62701 tccttaccaa cttcacctaa tatatatcct tttgtaaact ttctattaaa tatggtatac
    62761 aaattattta ttatttcctc agaaatactt atttttttct tatctacaaa ttcattaatc
    62821 gcttctctat atcctgaaac cacagtagct acatactctg gctttttcat tctaccttca
    62881 atttttaaag ataatacacc tgcttcaatt attttatcca aatcttctat agtacttaaa
    62941 tctctaggac tcaacaaata atctccatta ctttttacta cttctcctgt atttatatct
    63001 atcaactcat acttttgtct acatggctgt gcacacctac ctctatttcc cgacctattg
    63061 cctatcatac tactcataag gcattgaccc gagtaacata cacataaagc tccatgtaca
    63121 aaaacttcta tatctacagt tgtattttca caaatccatt ttatctcatc aacatttaat
    63181 tctcttgcta aaacaactct atcaaaccct acactctgaa ggtattttac atcttctaag
    63241 gaatgtgcta ccatttgagt gcttgcatgt agttcaaagt caggtaatag tttttttata
    63301 agtcttgcca ttcctatatc ctgtactatt aaagcatcaa tatctatatc gtataaaaat
    63361 tttacatact ctacaaaatc ttctatttca tactgtttta tcaatgtatt tacagtaaca
    63421 aagacttgca ctccccttat atgagagtat ttaacagcct caatcagctc atttctatca
    63481 aagttatttg cagatgctct agcactaaag tctttaccac ctaaatacac agcatttgct
    63541 ccgttttgaa ctgctgcttt taatgaatca aaagagccta ctggtgcaag taattctatt
    63601 tttttcatat ttttctcctc tatttatact tgttaggtta atataattat atctataact
    63661 taaatatttg tattaaattt atatatttct tactatattt taaattcttc aatcaattta
    63721 tatattagtt atttatattt ggttcatttg agttaacata tacattattt actatattta
    63781 gtatatttct attaacttat atattgatta ctgtatttta aattcttcaa ttaatttata
    63841 tactgcttac taaatttaaa acattt
//
LOCUS       DAELLV010000003        60674 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.003, whole genome shotgun sequence.
ACCESSION   DAELLV010000003 DAELLV010000000
VERSION     DAELLV010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 60674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60674
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.003"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            162..1430
                     /gene="eam"
                     /locus_tag="KPD58_000152"
     CDS             162..1430
                     /gene="eam"
                     /locus_tag="KPD58_000152"
                     /EC_number="5.4.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 2,3-aminomutase"
                     /protein_id="HBG0263947.1"
                     /translation="MNEQTRISLERAAELKSKIDDYIQARKTINRGLEKEEEINKRKQ
                     KILSILNGTEEDWNNYKWQLSNRITDVDTLSKIITLTKKEKEYIKEVGTQFRWAISPY
                     YLSLIDPEDICDPIKLLSIPTHIELEDEQEDLDPMGEEYTNPAGCITRRYPDRLIINV
                     TNECAMYCRHCQRRRNIGQQDSHKSKAIIQESIDYIRENEEIRDVLVTGGDALTLKDD
                     YLEWILSQLKEIPHVDYVRLGTRTLVTMPQRITDEFCNMLKKYHPIYINTHFNHPMEI
                     TKESKEACEKLANAGVPLGNQAVLLNGINNDKFVMRCLNQELLKIRVKPYYIFQSKHV
                     KGTKHFNTSVDDGLEIMEYLRGYTSGMAIPTYIVNAPKGGGKTPLLPQYLVSKGTDYV
                     MLRTWEGKVIKMEDEPAVDIKKLIKEQAQD"
     gene            complement(1523..1879)
                     /locus_tag="KPD58_000153"
     CDS             complement(1523..1879)
                     /locus_tag="KPD58_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0263948.1"
                     /translation="MKKALISLLVLSCLLLTAPVNESSYADSQKNVPVSQNTKLTQEQ
                     AKELLVKYNNEVDYIYQGNASDFEALKAKNLNGYVFLPDADGDIGYFVNENTSEIYYF
                     HPSGYLELVNQQQNNK"
     gene            complement(2030..3124)
                     /locus_tag="KPD58_000154"
     CDS             complement(2030..3124)
                     /locus_tag="KPD58_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG0263949.1"
                     /translation="MQDKKIYLNIVLTVVVSYILIKVIDNYKYFFGVISLLMSLLTPF
                     IIAFVLAYIFNPLVKFLESKLNFKRIYSLLLTYGVLIVILISFILFTVPSIVNSLADL
                     VAQIPTYIDKTEQFLFDLGKSLQSVDPNTLKEYGDKIMSVMPKFSNLLIGSLGGIFST
                     TFSVGKFIVQFLLGFIICFYILLEKEKFLLFTKKVVYISLGKKYGDFIIELCQSLNLN
                     IGKYFTGKILDSFIVGVLSGIGLYFLKSEYALLFGTLMGVMNMVPYFGPVIGMAPVVI
                     INLFSNPTIALTSLIYLIIIQQVEVTFIEPKIVGGQLGLSPFFTILAVTVGGGFFGIP
                     GMILSAPVMGVIKIYFCEFVNRRHDKIQME"
     gene            3814..6861
                     /locus_tag="KPD58_000155"
     CDS             3814..6861
                     /locus_tag="KPD58_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HBG0263950.1"
                     /translation="MKKILKRLCTGFLAFATVVTALPSSTVHASNTQYWTESKERVGI
                     VEQVMNDGSISSTFNEGHLTVEGKDAYCIDINTAFKNGYKTRTDASSRMSADRISDVA
                     LSIEYVKQYTDSHSGISSKHAYLLKQLVVWQRLSVQLDWQCDNVRASYDEIPKAVQDE
                     VFAGAKAFVKENKGRYDCGGYIYSGEGQELGQFWAKLAVGNAKLQKTSTNANITDGNG
                     IYSIAGATYGVYSDKDCTKQLATLTTDTSGNTEAVEVRATTVYIKELSAPAGFKIDKT
                     VYSLSVEAGKTATLKVSDTPKVTDTLIELFKIDMETQKSNSQGNASLEGAEFTWNFYA
                     GYYNKNNLPAQPTRTWVTKTIAEKDSDGAIHYITRLANAYKVSGDSFYMQDGKNVLPL
                     GTLTVEETKSPSGYLLEGAYMQADGSEEQIKEMYLTQITEDGDLAVLSGSNQYHVSDK
                     VIRGGVKIQKRDLETKDTKAQGGATLKNTAFEIISLNENAVLVESKLYKKNEVLKTIH
                     TDIEGIASTSADLLPYGKYRLSEQKPPEGYLTDGAKPIDFEITENGKIVDLTDEAHSI
                     YNQIKRGDIEGVKIGAGSHKRLADVPFRITSKTTGESHVVVTDDNGQFSTASDWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALIYDTYIIEELRCESNKGFKLIPPFEIVISRN
                     KVVVDLGTLTDEYEKEITIHTTATSKDGEKTILAGNDVTIIDTVKLDGLVKGTKYQLK
                     GWQMLKKENAELLVGDKRVESDYTFVADDEAMKVEIAYTFNASALGGKNLVTFEELYD
                     LSNPEEPVKVAEHKDIEDDGQTVLITERIIKIHTTATDKDGKKEIEAGKDVTIVDTVK
                     LEGLEVGTKYQLVGWQMLKEENAELIINDKKVENDYTFTSDSETMEVKIAFTFDASSL
                     GGKQFVTFEELYDLSNPDKPIKVTEHKDIEDDGQTVTIKEVPETPTPEEPEKPTTPDT
                     PTKTDSPKTGDNTNILAFAIMMFVSAGGLAGTYFFKRRKMKKS"
     gene            7083..7406
                     /locus_tag="KPD58_000156"
     CDS             7083..7406
                     /locus_tag="KPD58_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBG0263951.1"
                     /translation="MELKYIVPNVAQTFGNLEFAGENKVDQQHINGRYTPVSRSYNLY
                     SDIQRADDIIVVLPAKAGEKHFKPEQKVKLINPRITAEGYNVKGRGFTNYILMADDML
                     PAESK"
     gene            7421..7834
                     /locus_tag="KPD58_000157"
     CDS             7421..7834
                     /locus_tag="KPD58_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBG0263952.1"
                     /translation="MRLANGIVLDNEKTFGVLKFSALRHEVRVENEDGTTSDEIKRRT
                     YDLKCSVQERMIQVSIPADVPVKDFPYNAEVELINPVADTVANANFRGADVDWYIKAD
                     DIILKNKGNQPAGNPQNNNPQNLAPQGQQKNKLAN"
     gene            7915..8373
                     /locus_tag="KPD58_000158"
     CDS             7915..8373
                     /locus_tag="KPD58_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG0263953.1"
                     /translation="MKFKNPMLVVTDIDKSVEFYKKVFGLRVIMDFGANKTLTGGLAL
                     QTSETYKEFIGTSDISFGGKNFEVYFEEDDFDKFADRLKEYDIEYVHPIIEHSWGQRV
                     VRFYDPDKHIIEVGENMKIVCKRFLNSGMTPEQVAERMDVPMKFINACMR"
     gene            8437..8850
                     /locus_tag="KPD58_000159"
     CDS             8437..8850
                     /locus_tag="KPD58_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBG0263954.1"
                     /translation="MLDKIPNAEEMTALVGQSLYAIWNKLCALIDEKYDMECLWNKGG
                     KAWTYEYKYRRGGKTLCALYARENCVGFMIILGKDERLKFDTDRNSYSKEVQKIYDET
                     KTYHDGKWLMFEPTDTILFNDFVKLLGIKRKPNRK"
     gene            8905..9507
                     /locus_tag="KPD58_000160"
     CDS             8905..9507
                     /locus_tag="KPD58_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0263955.1"
                     /translation="MDKVVIYIHGKGGNAEESSHYKPLFSDCDVVGLNYTAQYPWEAK
                     EEFPSLFDLICKHYKSVQIVANSIGAYFAMNALSDKQIEKAYFISPIVNMEKLIADMM
                     YWANVTEDELRDKKEIKTTFGETLSWEYLCYVKENPTTWKIPTNILFGEKDNLTSYAT
                     ISEFTKQTNATLTVMRNGEHWFHTEEQMKFLDEWINKSAR"
     gene            9568..10110
                     /locus_tag="KPD58_000161"
     CDS             9568..10110
                     /locus_tag="KPD58_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBG0263956.1"
                     /translation="MKGFERKNQLFSLCGLNCGLCPMLLGNYCGGCGNGNQSCRIAKC
                     SLEHGQIEYCYECKQYPCEKYQHIDDYDSFITHKQRKADLERAKNIGIEQYNLEQQEK
                     TQILSYLLSNYNDGRRKTFFCVAVNLLELSELQEAMKQIQSNDELTLLPFKAQCLYVV
                     EVFQKIAERRNIKLKLFKKK"
     gene            10138..10449
                     /locus_tag="KPD58_000162"
                     /pseudo
     CDS             10138..10449
                     /locus_tag="KPD58_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896960.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
     gene            10560..11954
                     /locus_tag="KPD58_000163"
     CDS             10560..11954
                     /locus_tag="KPD58_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0263957.1"
                     /translation="MRIIWNKGHRIRASDKNLVYRFSLGTLLIVFVVVLLLLNMKQLM
                     RTDWEHFSLLDNSFTLSLYNFITLLIATGVCALVAFLYYRFCYDNFKKLLHRQKLARM
                     ILENKWYEADTVQDSGFFTDLQSRSREKIVWFPKIYYQMEKGLLHIRCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIKYILLYDMIANRITIDEVRAENGCLRLMKNLV
                     WEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMGNVY
                     HTKEEMIDCVNAFYEGMVQRSEEMKQHPNYKTGENYAYLSLPPCFLIFDEYVAFFEML
                     GTKESVSLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSIISEFYTPLVPKGYDFLQTIGSLAYARQ
                     DGTATCEAKGDGTD"
     gene            12156..13355
                     /locus_tag="KPD58_000164"
     CDS             12156..13355
                     /locus_tag="KPD58_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBG0263958.1"
                     /translation="MNETDFLTALKAKRCAYSVSQTRLAIMANISREHLSRIEAGKVT
                     LTEDMKHTLLEAVEKFNPDNPMFLLFDYVRIRFPTMDIKHIIKNILKLNIDYMLHEDY
                     GHYKYTEHYHIGDVFVYVSQDEEKGVLLELKGKGCRQFESYLLAQERNWYDFFTDALV
                     EGGVMKRLDLAINDRTGLLDIPELIQKCENEECISKFRSFKSYGSGELVKHNETDKYG
                     MGHTLYIGSFSSEVYFCCYEKNYEQYAKLGIPIEEVPIKNRFEIRLKNERAYYAVVEL
                     LTHYDAEQTAFSIINQYIRFADKEPDKRKSDWKTNVRWAWFIGEGRPPVKLTTKPKPY
                     TLERTLNWLQRQVAPTLKMLKQIDKDNQTEYLETIEKQAKLTERHHQIIKQQTATVEE
                     MVAKVKE"
     gene            13362..13496
                     /locus_tag="KPD58_000165"
     CDS             13362..13496
                     /locus_tag="KPD58_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBG0263959.1"
                     /translation="MWELLKDFLLVSLGMGIGVVLMCILTVGKEADRCMEEIKEREEH
                     "
     gene            14238..16148
                     /gene="ltrA"
                     /locus_tag="KPD58_000166"
     CDS             14238..16148
                     /gene="ltrA"
                     /locus_tag="KPD58_000166"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBG0263960.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGTPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMPDGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWHENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKTKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKTQKDKLVSQAKKI
                     ASPSKGKTELEEIRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGASKKG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYSANCYTPEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTRAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            16315..16476
                     /locus_tag="KPD58_000167"
     CDS             16315..16476
                     /locus_tag="KPD58_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0263961.1"
                     /translation="MAIVVIGIYLGFKREFSKLIGFLVVALVAVGLVFNAGGVKDVLL
                     ELFNKIIGA"
     gene            16557..16934
                     /locus_tag="KPD58_000168"
     CDS             16557..16934
                     /locus_tag="KPD58_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0263962.1"
                     /translation="MDYKVDDKNLDASMFISFVNQVWQGDYDMEQTRQALSKTLNITA
                     YDNKKLVGCLRILSDGYYFGTITELLVLPQYQKQGIGSKLLQLAKDNTPTMLYFGSQP
                     EAEKFYEKNGCQKSLQSYMINKG"
     gene            17041..17337
                     /locus_tag="KPD58_000169"
     CDS             17041..17337
                     /locus_tag="KPD58_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG0263963.1"
                     /translation="MNDIFKNMQAKIGCEYLSDLPSYKRKVWHEMKQLNLADYEEKQL
                     EDFSKYVFGMSYQTLKDVMKQQIGREEQCRKQGYWWKRKEQLTKKQHHTGSTCR"
     gene            17256..17759
                     /locus_tag="KPD58_000170"
     CDS             17256..17759
                     /locus_tag="KPD58_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBG0263964.1"
                     /translation="MQETRVLVETKRTTDEETTSYWFDLPIEVAEFEEKLGVGAESGE
                     YRIIEKVLPYADEVHEHTSVYQLNKLDFMYRQLSSDMQEEYVSLLMVFENLEALYICR
                     NVITVYPDCKSMIDVARQKLMNDPTFKHLSEDCQEYYFNFEAYASHLQEHGKFLVTEH
                     GIFELPE"
     gene            17777..18280
                     /locus_tag="KPD58_000171"
     CDS             17777..18280
                     /locus_tag="KPD58_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBG0263965.1"
                     /translation="MIDDMAVYIANLGKYNEGYLVGAWFSFPIDEEDVKEKIGLNEQY
                     EEYAVHDTDNFPIDIGEYVSIEELNEMYEMIEELPDYIVECLDEFISHYGTLEEVVEH
                     KDDIYYYPDCETMTDVAYYYIDELQALGDIPPSLQNYIDYEAYGRDLDMGGCFIETSR
                     GMCEIPY"
     gene            18401..18799
                     /locus_tag="KPD58_000172"
     CDS             18401..18799
                     /locus_tag="KPD58_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG0263966.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDMPPLSMIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITYALRPKITY
                     AGKAVKLHKQTLNETITAVRSVNYVPDKIY"
     gene            18777..21227
                     /locus_tag="KPD58_000173"
     CDS             18777..21227
                     /locus_tag="KPD58_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0263967.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSIRSMQEQSKKLVTGKLREVAVQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTEEQLNLKNIKKSAWLTFTEFLHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLEINDFGYLMEHLYGRDGIAYEDYEYQLPEKKLQKE
                     TLIKYYDLIRPTRCVIEESQRYLRLEHEDKESYVSYFTVNAIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALSTVRNKKKELKDLDNHAYQAGSETSSNVVDALDSVD
                     ELETDLDQSKESMYKLSYVIRVSAPDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGENTGIYMGYSVDTGRNVYLQPS
                     LASQGVKGTVTNALASAFVGSLGGGKSFCNNLLVYYSVLFGGQAVILDPKSERGNWKE
                     TLPEIAHEINIVNLTSDKDNAGLLDPFVIMKNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRSVTQSDSRGLLHVIDELRREDTPISRNIADHIDSFTDYDFAHLLFSDGTV
                     ENAISLDNQLNIIQVADLVLPDKDTTFEEYTTIELLSVSMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWAFLNVAQGETLSNKLVRAGRAMQAGVYFVTQSAYDVSKESLKNNIGLK
                     FAFRSTDINEIKQTLEFFGIDKEDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFE
                     ELLHAFDTRPPVQRNEVE"
     gene            21227..23422
                     /locus_tag="KPD58_000174"
     CDS             21227..23422
                     /locus_tag="KPD58_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBG0263968.1"
                     /translation="MKERIQSAFTKKKMFRFLKITVFVVALSLILLSLLETVAHATGL
                     VDDTINTENLYSKYPLSNYQLDFYVDNSWAWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGQSIQTLAGVTSSGFSTSGFYVGFLLLIIL
                     VVGIYVAYTGLIKRETSKALHAVINFVVVFILSASFIAYAPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSEGKDSVDLIRDSLFSIQVEQPWLLLQFGNSNAEEIGTDRVEALV
                     SVSPEDEDGKTREEIVKTEIEDNDNNILTIPQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVCFAGIYMKLGDLMSMFSLNASDSQSMGRRIFRRP
                     YLFIRHRARRMERRMARAVSAGGISGGVAGALAGSTVSNARTTRNNPTKENRGNTTSI
                     MGQRAGSKIGAVLDTKNKVKDKANAVKENVKDMPTQTAYAVYSAKEKAKSSVSDFKRG
                     MVQEQQSRQTGRLEKQEQRKKNIANKRMELQKAQEARQARKADGSATTGATRPHERPA
                     TASKPSVEKVQEVKRPATTPTSKASEPVKTNVIKERPLSSGASDGKATQSAQPIHRQN
                     IEKVVSQETRQNYVKDRRTKVQQTQSVQKNQQTTEKNHNLVTKKGQKKK"
     gene            23419..24423
                     /locus_tag="KPD58_000175"
     CDS             23419..24423
                     /locus_tag="KPD58_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBG0263969.1"
                     /translation="MKLKHIALIGSLFPILFSIVLFFGVLISADSDDENSNFSSGITG
                     LNLSAEVLKHQPMVEKYAREYGISEYVNVLLAIIQVESGGTAEDVMQSSESLGLPPNS
                     LDTESSIKQGCKYFASLLSSYKNQGINDLNVAIQSYNYGGGYVGYVAGKGKKHTFTLA
                     ESFACEKSGGKKVTYTNTIAVAKNGGWRYGYGNMFYVELVNQYLTVPQVSGELAQKIM
                     NEALKYQGWKYVYGGSNPNTSFDCSGLVQWCYGKAGISLPRTAQAQYDATQHIPLSQA
                     KAGDLVFFHSTYNAGSYVTHVGIYVGNNQMYHAGDPIGYADLNSSYWQQHLIGAGRVK
                     "
     gene            24443..25345
                     /locus_tag="KPD58_000176"
     CDS             24443..25345
                     /locus_tag="KPD58_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG0263970.1"
                     /translation="MFKKSKKQTENVKEKKVRTVKVGTHKKTVIAFWVVLIVSVSFGV
                     YKNFTAIDMHTVHETETIQLRLTDTNGIENFVKNFAKSYYTWNNSKEAIEARTQAISG
                     YLTKELQDLNVDTIRTDIPTSSTVTDVIVWNIEQSGTDTFSATYEVDQQIKEGEQTTA
                     VKAIYTVKVHVDVDGDMVIIQNPTLAPTIEKSDYELKTPEADASVDADTINDATSFLE
                     TFFKLYPIATEKELAYYVSGNVLEPIGKDYLYSELINPVFTKDGDNVKVKVAVKFLDN
                     QTKATQVSQYELVLHKDSNWKIVK"
     gene            25489..26100
                     /locus_tag="KPD58_000177"
     CDS             25489..26100
                     /locus_tag="KPD58_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0263971.1"
                     /translation="MPKGIMLTQEQQEERREEIISVALQLIEKNGFQKTSMRKIAILA
                     NMGKSSLYDFFKTKDEIFVYAVEKEIEETIKKVHRIIADESSPEQCLRKIMLNHLGVP
                     KQYRTVLMWLNTESDYLEEDYRKRLKTARYAYQDIIKSVIENGMESGVFRKTNANLMT
                     RLLINSIISIIYTSRPSASPEKMLDETMDIFLHGIMNDGGELL"
     gene            26097..28664
                     /locus_tag="KPD58_000178"
     CDS             26097..28664
                     /locus_tag="KPD58_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HBG0263972.1"
                     /translation="MIYYTLPLLHKQATLEMVGGKGMSLSKLLTTGIPVPEGFHVTTT
                     SYKIFVEKNHIQPYINKLLDGIDTNNTSQLENVSTQIGMLFHNGEMPQEVSDAIKTAY
                     ARLGNIAVAVRSSATAEDLPDASFAGQQETYLNIQGENEVLDAVKRCWASLWTARAIA
                     YRVKNDIKQEIVALAVVVQKLAFSDASGVMFTLNPINGRRSEMIVNAAWGLGESVVSS
                     LVTPDTIVVDKNAERIVSYEVANKEIMTVRNSDGTEEIPTPEQLRKKHALTRNQVMRL
                     TQLGKKIEKYYEMPMDVEWALEKDKLYIVQARPITVLPPEWTLPEKDVIYTRGSLAEH
                     LPNPVTPLFATLGLEIVNRASALLWIDMFGKSAKKLLPENGAYTIINGYVYLSANSKP
                     LLIAVKSLSPRSLRRALTNSVARWETARKEFEDVIKQWEEKPMHMLNAHQIMEGIQTV
                     FYGACIYFTRIQLTLPAASISETLFTKFFQGAARRAGMTDTSVFLLGFDTIALQAEKN
                     LWSLSEWVKQNNTLNLYLQSNPTAKIAENFMSSVPPDEVSLEVWIEWKNRINQYFKEF
                     GRTAYEFDFAYSTPQETLTPTFESIKTFVEGKGESPFLRQSKFEKHRKQAEEEILQHI
                     GSSRKKLFLKLLHWAQETAPMRENAIYCMGMGHPLIRQMFHEISARLIRGGATSHIDD
                     IYWLTKLELEKLIVQLDKNIPLSDRRNSILARKAELKKYQGYMPPAQLPEKKVKSISH
                     APQKQKDGKTVLRGIGTSSGVVTARACVLNSPADFKNFQPGSVLVAVTTTPAWTPLFA
                     SASGIVTDIGGPLSHSSIVARECGIPAVMATHTATRSIKNGQMITVDGSEGTVTIDE"
     gene            28657..29817
                     /locus_tag="KPD58_000179"
     CDS             28657..29817
                     /locus_tag="KPD58_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0263973.1"
                     /translation="MNKKPIFALNLATLVIALGYSLILPVMPFYMENLGAGGRELGWL
                     TAVYALAQTVCAPFWGALSDRIGRKPIISIGIIGYSISLFLFGLASSFWTLFIARSLS
                     GILSSATSVASLAYVGDSSSEEERSKNMSQLGASMSVGVMMGPLLGGILAGYSLALPF
                     FTGAGISFIAFLLILTLLPESIERSGQTEKRKPFDFSDLKSIIFGSAGMVLILIFVVH
                     FCQTGLQGITGLYVVDKFGFSTKQVGVIWMVLAGILIVVQGFLTGPLAKKIGEKRLIL
                     IGMFCKALVAFAMVLTTGFVSVLVVFGLFAVSSAITVPTLNATLSKTDNQHKGTLMGF
                     ASTAGNLSKVIAPLLTGYLYESNIELPYITTGGIALIGVVICYIWIKNQKNK"
     gene            complement(30004..30197)
                     /locus_tag="KPD58_000180"
                     /pseudo
     CDS             complement(30004..30197)
                     /locus_tag="KPD58_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            30300..30806
                     /locus_tag="KPD58_000181"
     CDS             30300..30806
                     /locus_tag="KPD58_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBG0263974.1"
                     /translation="MKNEKKITVALLIVYLLILSWIILFKLQFSFSVLDHIRQINLIP
                     FGGSVIANGKIDFDEIINNAIVFIPVGAYFSLLFKNKSVLKAIGSVLGISFVYEIIQF
                     IFAIGASDITDLISNTLGGIIGIALVYVLSIVLKDKTHKILNRIAIVCTVLVVAFLFM
                     LLAVNNIF"
     gene            30811..31086
                     /locus_tag="KPD58_000182"
     CDS             30811..31086
                     /locus_tag="KPD58_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4865 family protein"
                     /protein_id="HBG0263975.1"
                     /translation="MLITCCFGKDFNLSKFAFEIEHEITPMEQMKAINFSLEKDDCTS
                     KVLIYNPDKWKYVEYYFYKDRPIKKLLEKFIQFCICHNKICFEILNT"
     gene            31356..31928
                     /locus_tag="KPD58_000183"
     CDS             31356..31928
                     /locus_tag="KPD58_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG0263976.1"
                     /translation="MNVDTIRTDIPTSSAVTDVIVWNIEQSGTDTLSATYEVDQQIKE
                     REQTTAVKATYTVKVHVDADGDMVIVQNLTLAPAIEKSDYEPKTSETDASVDADTVND
                     ATAFLETFFEVYPTATEKELAYYVSENVIEPIGRDYLYSELVNPVFTKDGDNVKVKVA
                     VKFLDNQTKATQVSQYDLVLHKDSNWKILG"
     gene            32031..32846
                     /locus_tag="KPD58_000184"
     CDS             32031..32846
                     /locus_tag="KPD58_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBG0263977.1"
                     /translation="MFKIGEFSKLTQVSTRMLRYYDETGLLKPAKIDSLTGYRMYSAE
                     QISTLNRIIYLRDSGFNVAEIALALNFSNNAIIEQLDRKSIEIMDNIQKEQEKLKKIE
                     IAKKEVLHGKSELYFNITIKEIPSYCVLSLRKEIPNYYYEGELWKELSVFAKENKINI
                     SDETFSIYHNEEYKEKDVDVELCAIVNKIGKATGDFKFRFTEPVSMMACTMVYGDFFN
                     ISYAYRSFAKWLQENSQYKMKGSSRQIVHRGPWNEKNPENYLTEIQIPLENLL"
     gene            32927..33403
                     /locus_tag="KPD58_000185"
     CDS             32927..33403
                     /locus_tag="KPD58_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBG0263978.1"
                     /translation="MITYKVNSIGTIYNNEKGAFIQLEAAYIPALRALDGFSHINVLW
                     WFSDFDNTDFRSVLETEQPYKNAPEKMGIFATRSPVRPNPIALSVVEIINIDYMNGMI
                     QIAYIDANDKTPILDIKPYTPSLDRVENPNVPDWCSHWPHSLEESASFAWENEFNF"
     gene            33417..33968
                     /locus_tag="KPD58_000186"
     CDS             33417..33968
                     /locus_tag="KPD58_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0263979.1"
                     /translation="MSLQDYIIEKPVIDTEHLILRTMDKNDIDDLKEWISDISLYEYW
                     GKRPSKSEKNPEVLFSKPEKATKSFHWGIVHKQDSKVIGEIWIYLIENNRMAKVAFRL
                     SKQYQGNGLMSEALKSVIEFCFSKTELQKIWTDVHILNHASYKTLEKVGFQREGHIRG
                     GKMVNTYCDYYLYGILKSDYITS"
     gene            complement(34358..35527)
                     /locus_tag="KPD58_000187"
     CDS             complement(34358..35527)
                     /locus_tag="KPD58_000187"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBG0263980.1"
                     /translation="MFYIGIDVGKFNHCACVISSEGEVLIEPFFFTNNLDGFDSFIKA
                     VKKFKSPRHIVGLEATGHYGDNLINFLITHDYEVALINPLTTDAKRKSKIRKTKNDKK
                     DTFLICSVLFDRDYTRVTQRKLDMKQARELTRYHASIAEDINQKKNQLQKCIDIVIPE
                     FNTWFKTKYSKAYMAVLKEFGSSYNLASANLAHLKKVLKPKSSGSRVNIDASSLRSSA
                     KKSIGENNEAVTLEIKMLIENIELLASQLKTVDKKIEELAVKLDSPIFSIPGIGIYTG
                     MSILSEIGDIHNFSSAVKVMGFAGVDPGTYQSGEYSAPRTALSKRGSRYLRKSLYQCI
                     LTVCKYNLTFNKYYKLKRLQGKSHRCAQGHSVRKLIRVIYKLLSENIQFDEKLLI"
     gene            35806..36717
                     /locus_tag="KPD58_000188"
     CDS             35806..36717
                     /locus_tag="KPD58_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG0263981.1"
                     /translation="MYAYENIEMSLNYIEQHLSEKIETEKLAEIACLSTFYYQRLFKK
                     LVKKSVQEYIKLRRLAMVIAELNNSEERILDIALKYGFSDHANFTRAFKEVYHITPDE
                     YRKNVPMLNTFDKPELSSRYIMIDENVPLIVGNIVLEIQRKTLRTKETYFGFEKTVNI
                     AEQIPAGESTGIDVPGQLWREFHAKKLQTENAIFNNDIELGVSHLASPDKGTFLYFAG
                     GMVSSTDVCPKNMVQHTLPAGEYIVCKIEAESFEELVTTALNQANKYLFETWLPNHNL
                     VTKPFMAEKYYKGNAEINYMEVWIIPV"
     gene            36795..37229
                     /locus_tag="KPD58_000189"
     CDS             36795..37229
                     /locus_tag="KPD58_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBG0263982.1"
                     /translation="MINIKDINYVPNISEISVYIENSAFDELYQHMNNEYKAVCKIEY
                     SKDVWARGWNVKFKKSGKSLCVVYPKKNYFTVLVVVGNKEKEDVENLLPRLSEYVQEI
                     YRNTREGNGQRWLMIDLKSKNNVYQDVLRLIEIRYAIKCVKP"
     gene            37697..38437
                     /locus_tag="KPD58_000190"
                     /pseudo
     CDS             37697..38437
                     /locus_tag="KPD58_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            38388..38999
                     /locus_tag="KPD58_000191"
                     /pseudo
     CDS             38388..38999
                     /locus_tag="KPD58_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            39212..40069
                     /locus_tag="KPD58_000192"
     CDS             39212..40069
                     /locus_tag="KPD58_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0263983.1"
                     /translation="MYKEVGISSNNVPITLSIWYTERSSPTIVFLPGTMSHPLMYENF
                     LCGLSERGFNIIGIHYLSHGKSPKIKKNYTMEDLLHNVYDATTYGIENFGENIAVMGS
                     SQGGILAAMAAGRDTRLKAVFPHNIMLTTLKETMSLTKYPTWSHRFMGLIQWGFRVGG
                     KLLPNYKVPGDAYLDYDRVFYSEQAKQDCLNDPMLLPYYPLRFVASLFNADLSCLENG
                     AIQCPVILITAKGDPLFSFDYTNKVFDLIHVPQKELLALELNRHMIFNEDTDTTINAL
                     EATLHKYLG"
     gene            40147..40728
                     /locus_tag="KPD58_000193"
     CDS             40147..40728
                     /locus_tag="KPD58_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0263984.1"
                     /translation="MPRFTEQEKEIINSKLLIEGEKLFALHGLKKVTVDDLVAAVNIS
                     KGSFYAFYPSKEHLYVEINFRLQKELFTSIETTIKKKKYKNHRDLAKDVIMLSLTGVI
                     TSPILSQIDLSLMDYLQRKISSDIFENHMYSDIRILEILEDLGVVFLVPHTVIIKSLY
                     SVLSCLEQFKEDEELNMIQNLLVNGIIQQVVAE"
     gene            40832..41677
                     /locus_tag="KPD58_000194"
     CDS             40832..41677
                     /locus_tag="KPD58_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0263985.1"
                     /translation="MIDVKKLYFSYTDKPFVENVSFHVGRGEIFGFLGPSGAGKSTIQ
                     KVLTGLNTRYKGSVKVAGTEIREHTNQFYENIGVDFEFSTCYEKFTARQNLAYFASLY
                     EKQPRSIDELLHMVGLENDGDKKVADFSKGMRSRLNFIKALVHDPDILFLDEPTSGLD
                     PTNSRLMKDIILAEKKRGKTIIITTHNMFDATELCDQVAFIVAGKVSALDSPHNLIMS
                     RGAAKIQYTYYDKGEKTGECLLDRTTEDKLLKSLISENRLLSIHSSEPTLNDIFVDIT
                     GRTLQ"
     gene            41674..42357
                     /locus_tag="KPD58_000195"
     CDS             41674..42357
                     /locus_tag="KPD58_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0263986.1"
                     /translation="MRLGRLICGDIHFQWKYGFYFIYFILTVLYVCGIAALPEHWKTD
                     IASIMIYSDPAAMGLFFMGAIVLLEKSQKVLNAMVVSPVKVSEYILSKTIALIAISTV
                     IALILGVVSDSNHLLGITVGTALTSAIFTMLGIIAATKISNLNQFLIVIMPIEIVCFV
                     PPIVGLFVKLPYLFRFFPFTACMNLITGKSVLLSFDMVLVIATLIILYIVARHTVKHM
                     WKSLGGVKL"
     gene            42354..43052
                     /locus_tag="KPD58_000196"
     CDS             42354..43052
                     /locus_tag="KPD58_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0263987.1"
                     /translation="MKRILSSTIQVFRQIKSDPMMFAACFTPFVMGALIKLGIPFLER
                     ITDFSLQAYYPIFDLLLSIMAPVLLCFAFAMITLEEIDDKVSRYFSITPLGKSGYLFT
                     RLGVPSIISAVIAFIVLLLFSLEKLSIGMTICLALLGSVQAIIVSLMIITLSSNKLEG
                     MAVTKLAALTLLGIPAPFFIDSYYQFAVGFLPSFWVAKAMQNEAVLYFSIGLVVALVW
                     YYFLTKRLFRKLAG"
     gene            43064..43975
                     /locus_tag="KPD58_000197"
     CDS             43064..43975
                     /locus_tag="KPD58_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBG0263988.1"
                     /translation="MEKGRLLQNGKVNRAIQVSKITLFINLSLSLLKFTAGYIGKSSA
                     MLSDAVHSASDVLSTIVVMVGIKISEKQPDREHPYGHERMECAASIILSVALAITGAG
                     IGYSGIKKIFSEQYNTLSTPSGIALTAAVLSIVIKEWMYWFTRNAAKHTNSDALMADA
                     WHHRSDALSSVGSLIGILGARLGYAILDPIASVVICGCILKAALDIFKESINKMVDHS
                     CDNATETKIREVVLQQQGVDGIDELKTRMFGAKMYVDIEILADGNLALYDAHRIAEGV
                     HQTIENNFPQCKHCMVHVNTNELLYSA"
     gene            44027..44167
                     /locus_tag="KPD58_000198"
     CDS             44027..44167
                     /locus_tag="KPD58_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0263989.1"
                     /translation="MEQYWWIIIVVVLIVVGLLKIVIMKKYNQRRNSNNSPKDSEDDK
                     MY"
     gene            44457..45083
                     /locus_tag="KPD58_000199"
     CDS             44457..45083
                     /locus_tag="KPD58_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0263990.1"
                     /translation="MSDTKENILKIALHLFAVNGYEAVSVSMIAEALGMTKGALYKHY
                     KNKQDIFDCIVERMYQIDNKRAKDYQVPESTIEVTPNAYRNTTIGSLITFTLAQYDFW
                     VTDEFASNFRKMLILEQYRNLKVNMLYQKCLVNGPISYIEDIFNEMIEKGVLQECDTK
                     QLAIEYYAPLFLLISVADAVENCWDLKTQLTTHVKQFVKKYIINEKVK"
     gene            45205..45921
                     /locus_tag="KPD58_000200"
     CDS             45205..45921
                     /locus_tag="KPD58_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG0263991.1"
                     /translation="MEYIRSKKYFTPDLQTKIMGPNPIKLQEELLQNHKIPSGSYVCD
                     LGSGQGLTSVFLAKEYGFVVFAVDLWSEPKENEEFFKKMGLSSAQIKSVKADANELKF
                     EKEFFDAVVSTDSYNYFGRDPIYLDEKLLPFVKKGGFVYITVPGMKKDCHEKLPEELL
                     LSWTPEELDYIHDITYWSNIISHCKKADLISIYEMESNSEVWSDWIKQNNEYAINDRK
                     AIEAGGGDYLNFIAIILQKK"
     gene            46085..46276
                     /locus_tag="KPD58_000201"
     CDS             46085..46276
                     /locus_tag="KPD58_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HBG0263992.1"
                     /translation="MKKYEYVEINYNAKGVVFLCTDKHKEIIDSYAKTGYHYAGFVPT
                     EIDAKGCMRKIDLIFEKED"
     gene            46300..46530
                     /locus_tag="KPD58_000202"
     CDS             46300..46530
                     /locus_tag="KPD58_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0263993.1"
                     /translation="MIGLKRRNMNYIMKVGAKAQGVSVSEFRAMLQDTIDKTMESTDP
                     DVQANFKRYFGNKRPTPEEYIYTITKKTKVKV"
     gene            46797..47216
                     /locus_tag="KPD58_000203"
     CDS             46797..47216
                     /locus_tag="KPD58_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG0263994.1"
                     /translation="MKPSDFQKTIQCQFECKLKKVVKGIIWNYRKELKRRKNKEISFS
                     ELPDITVESLATWDDYETNYTIFNVCGIDIKVLDDELAEALKQLPEKKRNNVLMYYFL
                     EMSDTEIADIMNYTRGAVFKHRHRALETMKEILKEKK"
     gene            47219..47473
                     /locus_tag="KPD58_000204"
     CDS             47219..47473
                     /locus_tag="KPD58_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0263995.1"
                     /translation="MKKKYKHPSYYLISSAMDGDETAIEKLLVFYDPYISKCCLRPLY
                     DEYGNVYIVVDMELKGRIREALIKMILDFEIDIDIETEEE"
     gene            47845..48048
                     /locus_tag="KPD58_000205"
     CDS             47845..48048
                     /locus_tag="KPD58_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBG0263996.1"
                     /translation="MNNTDVPIWEKYTLSIEEASRYFRIGEKKLRRLAEENLDSGWVI
                     VNGNRIQIKRKQFEKIIDTLDEI"
     gene            48126..49316
                     /locus_tag="KPD58_000206"
     CDS             48126..49316
                     /locus_tag="KPD58_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG0263997.1"
                     /translation="MSKVKRQDSKGRNLRLGESQRKDGRYVYKYTDIFGKPQFVYAWK
                     LVPTDKTPAGKRDDISLREKEKQIKKDLDDGIDTVGGKMTVCQLYEKKNNQRKNIKRN
                     TKIGRQYLMNALENDPLGMRAIDTVKQSDAKEWAIRMNDKGYSYKTIDNYKRSLKASF
                     YMAIQDDCIRKNPFNFKLSDVLEDDTEAKVILTPEQEEKLLAFMETDNVYSKYRDEVI
                     LLLETGLRISELCGLTTHIDMLNRVININHQLLRDTEVGYYISTPKTKNGKRKLPLTE
                     RAYQALERILKNRGKAQPLVVDGYSNFLFLNREGLPKVAGNYESMVRGLIKKYNKTHK
                     DKLPNITPHSFRHTYCTNMANKGMNPNTLQYIMGHANITMTLGYYAHGTFQSAKAELE
                     RLAS"
     gene            49556..50377
                     /locus_tag="KPD58_000207"
     CDS             49556..50377
                     /locus_tag="KPD58_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBG0263998.1"
                     /translation="MIKKEISNFRGSSDYVVSPELMASVNVAIALEKPLLIKGEPGTG
                     KTMLAQAISNELKKDLVIWNIKSTTKAQEGLYVYDTVQRLYDSQFGGEGVDDISKYIK
                     YGKLGEAFSSNQQVILLIDEIDKADLEFPNDLLWELDKMEFYINETKETVRAKQRPIV
                     IITSNAEKELPDAFLRRCIFHYIEFPDRDMMEEIVKVHFDKVEEHLLEQVMTTFYWIR
                     SLKDIQKKPSTSELIDWIQALTLSGMPIEKIEKEVPFAGILLKNNEDIESMQRHL"
     gene            50398..51594
                     /locus_tag="KPD58_000208"
     CDS             50398..51594
                     /locus_tag="KPD58_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBG0263999.1"
                     /translation="MFIEFFYLLRSRGLNVSLNEWMTLIEALDKGLCYSNFSNFYYLC
                     RMILIKSESDFDKFDAVFLEYFKGIEHQEEIPEEIMNWLKKPDIDLEEFERLEQNPNI
                     NLDKLRAKLEERIKEQDSPHNGGNYWIGTGGTSELGHSGKGQTGIRIGGNSTYGRAVL
                     EVAGERKYRDFRDDEVLNMRQFQVALKRLRQFSTRIDAPKTELDLDKTIEETCNNAGY
                     LKLFFEKPRKNTVKLLLLMDSGGSMRGYSTLCNTLFQSVSKSNHFKDVKIYYFHNCIY
                     DRLFTTPECWLSKSINTEWILKNIDKNYKVIIVGDASMSPSELLHIGGNYRGPYNYTP
                     GIEWLKRFKRKYSKIVWMNPELRDGWDSINYWYQTQRMIQSEFNMFPLTVKGLEKALK
                     NLMVSR"
     gene            51762..55160
                     /locus_tag="KPD58_000209"
     CDS             51762..55160
                     /locus_tag="KPD58_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBG0264000.1"
                     /translation="MIIINYELIVKYNGDILRLEEELGVSVEILNSSYAIITSSNEED
                     VNILLTYPEIEFIEKPFILQTQDVQSFSSTGITGFKNRTGLTGKGTIIGIIDSGIDYT
                     LPVFRDSDGRSKILYYWDQSIQGNPPEGFREGTLYTNEDINNAIDGSMYIPISTTSLH
                     GTHVAGICATIASDARIIVVRVGNIQTDIFSRSTEFMRAIKFILDRALELRMPVTLNI
                     SYGSNEGSHRGTSLFEQYIDDMCLFWKNNIVVAAGNNADKGGHKRIRLQNNITEEVEF
                     IVGEGERILNINIWPDFVDDFSVHLVNPSNNQTQAISLTSGEIRNTLGETRITGYFYP
                     IAPYSLTRRVTLQLSSNTQITPGLWKIVFEPIDIVTGNVNIYLPTSEGLNRNTRFLIP
                     TQELTVTVPGTASRVITVGSFNSRTDIVSIFSGEGDTQLGVFKPDLLAPGEDIVSFLP
                     GGTSGALTGTSMATPHVTGVCSLFMEWGIVNGNDLFLYSQKLRALLLKGARRLSNQSY
                     PNNSSGFGFLNLSDIDLYTLSNINQDLETEDIGYRSINKSFKDEENSYKFIDGYNMQI
                     HNDLENEIYISKNASRQSGILSGIDIVHTPEFEEELAGLGMSQRFFKISDSLGVLSIN
                     NTDYNSIQRVLQLPSIIRTVSTTKMTLLGEINRGTFGGVVATEEMGVNFFKNNPNINI
                     TGRGTLISIADTGIDYLHPDFIYPDGTSKIVYLWDQTKEGTPPDGFYIGTEYTREDIN
                     RAIAENDPSLSQDEVGQGTMLSGICAGLGNVNSEYAGIAEDSELIIIKLGKIDDFYNS
                     AMLFAASQYAYKKAFELGRPLVINMSLGTSSLAGLTNRSNSEKAFFTRGLCITAGAGN
                     EGNTQTHTSGIIPYVGGSVEVELELNEDEEELSLELWLNRPDKADVIIVSPTGEESKS
                     VGISNYNKVTGLFDLEGTEYSITYIYPTTFSGQQFTNVTLKNAKRGVWKIRLVGVYII
                     TGRYNLYLPNRELLKSGTRFREVDPFYTINYPAIQDDLITIGAYNTINGSLWQSSSRG
                     PTIEDRLKPDIVAPGVNIIAAYPGNTYATITGTAAASAHAAGAAAMYFQYTFVDGRYP
                     NQAYVQKIKTFMQAGARKDSNTVYPNTNSGYGLLDVRGMFDVLR"
     gene            55170..56843
                     /locus_tag="KPD58_000210"
     CDS             55170..56843
                     /locus_tag="KPD58_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HBG0264001.1"
                     /translation="MEKSYCIIYQGDIESALQENGINRYMVLNSQLAVIYVPLDFDET
                     ILNNIIQVAWWEESEPMSSLIEITNNVNNGETITTAAETDYIYENPYNDITGRGILLA
                     VIDSGIDYLHPDFINDDGTSKVLYLWDQEANTNPPPEGFIFGSEFTRSELNIAINRND
                     GSLSQDNIGTGTLVSGILVGNGRINSQYRGITTESDLIVVKLKSYTDTYYAGRINYSV
                     SDFLAAITYVTNIARTENKPLIINLTIGVKSSAVATTSILDTFNILSSAGVVVVSGAG
                     NQGNTDIHYSGRFSSVGEVQDVIIQDGDDYALDITLNTNGPDKVGAQIISPSGEVSHD
                     IRYSPDFYIYRGKFNLENTTYAMRFIYPYITSGKENLEIRLRDIKPGVWILRLTSELI
                     INGEYDIYLPNKNLIAPDTRFLDPDSVATITMYAASDDVITVGTFNNKTDSMWIGSSK
                     GPIRGRGIKPDIVASGVDIISTYKNGTYNTGTGTGVSSSIVTGVLALLMEYLEKQDNV
                     PRLSLFTQVLKTYLILGATKLEIYTYPNVSQGYGILNLKNTIQQIANTL"
     gene            57116..57310
                     /locus_tag="KPD58_000211"
     CDS             57116..57310
                     /locus_tag="KPD58_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HBG0264002.1"
                     /translation="MESKKARALKGKDNKRLKSSLLVGNESTAAWADVEKLKPHSKVS
                     IPSIKGVEEAKDWVDNGSKL"
     misc_binding    57479..57712
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            57789..59186
                     /gene="asnS"
                     /locus_tag="KPD58_000212"
     CDS             57789..59186
                     /gene="asnS"
                     /locus_tag="KPD58_000212"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBG0264003.1"
                     /translation="MQKEFITVKELYREKEQYIGKTVKVAGWIRTSRVSKNFGFIELN
                     DGSFFKNMQIVFDEKVENFKEVGKLAISSSILVEGELVSTEGAKQPVEIHAKSIVVEG
                     ESDSSYPLQKKRHTLEYLRTIAHLRPRSNTFSAVFRVRSIAAYAIHKFFQERNFVYAH
                     SPIITGSDCEGAGEMFRITTMDLNDIPKTEDGKIDYTQDFFGKEANLTVSGQLNAEIM
                     ALSFRNVYTFGPTFRAENSYTGRHASEFWMIEPEIVFADLEDNMELAEDMIKYVISYV
                     LENAPEEMEFFNSFIDKGLLERLNKIVNSEFIRITYTKAVELLIESGHEFEYPVEWGC
                     DLQTEHERYITEQIYNCPVFVTDYPKDIKAFYMRMNEDGKTVRAMDLLVPGVGEIVGG
                     SQREEREDVLLDRINEMGLREEDYWWYLELRKFGTATHSGFGLGFERIIMYMTGMSNI
                     RDVIPFPRTPKNAEF"
     gene            complement(59359..60534)
                     /locus_tag="KPD58_000213"
     CDS             complement(59359..60534)
                     /locus_tag="KPD58_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG0264004.1"
                     /translation="MNLEKIKKFFGFPLDKKHKDNVEKELCNYLYHTGKIICIIIVIS
                     QIIMMISISAREGGPFVTPRRQWYFYMYVTLFCVSSVFMFVFSYLWNKKKDCCTFYIN
                     LWIIYVTFFCLWGCVITLLDQLGGNDLSVFSYIMLAAAAFSVLKFGESVIVFGGCFLF
                     LNLFLPGIQTIPGNEFSNIVNTFFITSLAIFVSTMLYRNRVFTYYDKLLISQQYEEIS
                     HINNLLNDKVMTDDLTKMNNRRYLEDILQKKLISHANQEPITGMMIDIDYFKQFNDNY
                     GHQKGDYCLQNIAKVILDFTSSENEHAIRYGGEEFFICIFNCNEADAKLKSECLRKII
                     ESCNFERDDVPLKCVTVSIGVYTKKIGKTITLNEFISNCDKALYTAKEQGRNCVAFY"
BASE COUNT        21229 a         9553 c        12272 g        17620 t
ORIGIN      
        1 aaatgtgcga aataacagaa aaaatgcaaa aattaattca atttcatttg ttataaaatc
       61 tatatggcaa aattaaatct gcctatcaga atgatataaa cacatcagaa aggaggacgc
      121 tatcaaataa tacaagtagt taactttgat agcaaaaaat tatgaatgaa caaactagaa
      181 tatccttaga gagagctgct gaattaaaat caaaaattga tgattatatt caggctagaa
      241 aaacgattaa cagaggtctt gaaaaagaag aagagataaa taaacgaaaa cagaaaatat
      301 taagtatctt aaatggaact gaagaggatt ggaataacta caaatggcaa ttatcaaata
      361 gaataacaga tgtagatact ttatcaaaaa ttataactct aactaaaaaa gaaaaagaat
      421 atataaaaga ggttggtact caatttagat gggcaatatc tccatattat ttgagtctta
      481 tagacccaga agatatatgt gacccaataa aattactgtc tataccaaca catatagagt
      541 tggaagatga gcaagaagat ttggacccaa tgggagaaga gtatacaaac ccagcaggat
      601 gtataactag aagatacccg gatagattaa taataaatgt aacaaatgag tgtgctatgt
      661 attgtagaca ctgtcagaga agaagaaata ttggacaaca agattctcat aagtcaaaag
      721 ctattatcca agaatctata gactatatca gagaaaatga agaaataaga gatgtactag
      781 taactggtgg agatgctctt acattaaaag atgattattt agagtggatt cttagccaac
      841 ttaaagagat accacatgtt gattatgtta gattaggtac taggactctt gttacaatgc
      901 cacaaagaat tacagatgaa ttttgcaata tgctaaaaaa atatcaccct atatatataa
      961 atactcattt taatcatcca atggaaataa ctaaggaatc taaagaagct tgtgaaaagt
     1021 tagcaaatgc aggcgttcca ttaggaaatc aggcagtatt attaaatgga ataaataatg
     1081 ataaatttgt aatgagatgt ttaaatcaag aattactgaa aataagagta aaaccttatt
     1141 atatattcca aagtaaacat gtaaagggaa caaaacattt caatacatca gtagatgatg
     1201 gtcttgaaat catggagtat ttaagaggat atacatcagg aatggctata ccaacatata
     1261 tagtaaatgc tccaaaagga ggaggaaaga ctcctttgct tcctcaatac cttgtatcaa
     1321 aaggaacaga ttacgttatg cttagaacat gggaaggaaa agttataaaa atggaagatg
     1381 aacctgctgt agatataaag aaacttataa aagaacaagc acaggattaa aaaaatattt
     1441 aaagtttata aaaagaaaca actttaaaat aaaaagaaaa tggatgtcaa atttatatca
     1501 atttgcatcc attttttatt agttatttat tattttgttg ttgattaact aattcaagat
     1561 atccacttgg gtggaagtaa tatatctcag aagtattttc atttacaaag taaccaatat
     1621 caccatctgc atctggtaaa aatacataac cattaagatt tttagctttt aatgcttcaa
     1681 aatcactagc atttccttga tatatgtaat caacttcatt gttgtatttt actaaaagtt
     1741 ctttagcttg ttcttgagtc aatttagtat tttggcttac aggaacattc ttttgagaat
     1801 ctgcataaga agattcattt actggtgcag ttaataacaa acaagataaa actaataaag
     1861 atataagtgc ttttttcata ctaaaaccct ctttcatgtt taattatata agattcatta
     1921 cttaataata ttgtatgttt cctataatta aatattacca ttaaagagaa taatattaca
     1981 gttacacagc tgtaactttt gattacaaaa tcgtaattaa attgtatttt tattccattt
     2041 gtattttatc atgtctacga tttacaaact cacagaagta tatctttata acgcccatca
     2101 caggagcaga tagaatcata ccaggtatac caaagaatcc tccaccaaca gtaactgcta
     2161 ggattgtaaa gaatgggcta agacctagtt gtccacctac gatttttggc tctataaatg
     2221 taacttcaac ttgttgaatt attattaagt aaattaaaga agttaaagca attgttggat
     2281 tggagaataa attaattatt acaactggtg ccatacctat tactggacca aaatatggaa
     2341 ccatattcat gacacccata agtgtaccaa ataatagtgc atattcagat tttaaaaagt
     2401 aaagaccaat tccagaaagt actccaacta taaaagaatc taaaatctta cctgtaaagt
     2461 atttaccaat atttaaattt aagctttgac atagttctat tataaagtct ccatattttt
     2521 tacctaagga aatataaact actttttttg taaacagtaa gaatttttct ttttctaata
     2581 atatatagaa acaaattata aaacctaaaa gaaattgtac tataaatttt ccaactgaaa
     2641 atgtagtgct aaatatccca ccaagtgaac caataagcaa gttactaaat ttaggcatta
     2701 cactcataat tttgtcacca tactctttta atgtattagg gtcaactgat tgtaaactct
     2761 tgcctaagtc gaataaaaac tgctctgttt tatctatgta tgtaggaatt tgagcaacta
     2821 aatcagctaa actatttact atggatggaa ctgtaaataa tataaacgaa attagtataa
     2881 ctatgagtac accataagta agaagtaacg aatatattct cttaaaattt aatttacttt
     2941 ctaagaactt tactagtggg ttgaatatat atgctaatac aaatgctatt ataaatggtg
     3001 ttagtagtga cattaataaa cttatcactc caaaaaaata tttgtaatta tcaataactt
     3061 taatcaagat atatgaaaca actacagtga gtacaatatt tagataaatt tttttatctt
     3121 gcaaaataac acaacctttc ttatatttat taaatttaga tatacttaaa tctaagttaa
     3181 agttaatatt tatagcttat tatacaataa aaagcagtat taatatagta tgattaatat
     3241 gaaattataa atacgttaaa actttatgtt tatagctaaa ataataaaaa ttatttatat
     3301 tgtgtttata tataatctaa aatgaaaaaa agtggtgcct ttgattaaac taagttttta
     3361 taatttaaat tttgtacaat aagctatctt ttattattct ttaaaatata tatttcattt
     3421 aattatgaca aataaagata aaaattaaat attttagagg tgattttatt tagatataga
     3481 attttagaat aatactatac aaatgtattg tattattatt tttatatcaa aaacacacta
     3541 aaaatttgat tatccgctaa aacgctgtat ctatcggcat tatcaaggag ctaaacacat
     3601 caaaatacta ctaatttact acgattgaat gagccttgat acgtcctatt tcccagatat
     3661 gcaaagatac ggtacagcac atcagtattg aaacaagaaa aattgaatac ttcatagact
     3721 acggaacgca caatatcagc gttccttttc tatttccagc cgttcctagt ggacggctat
     3781 tttactacca aaatgaaagg agcatttaga attatgaaaa agatattaaa acgattgtgt
     3841 acaggctttc ttgcctttgc aactgtcgtt accgctttac cgagttcaac ggttcatgcg
     3901 tctaatacac agtattggac ggaaagtaaa gagcgtgtcg gtatcgtaga acaagtaatg
     3961 aatgacggtt ctatcagttc tacctttaat gagggacatt taaccgttga gggcaaggac
     4021 gcttattgta ttgacatcaa cacagcgttt aaaaacggct ataagaccag aacagacgca
     4081 agctcacgca tgagtgccga ccggataagc gacgttgcct tatctattga gtatgtgaaa
     4141 cagtacacag acagccacag cggaataagc agtaaacacg cctacctatt aaaacagctt
     4201 gttgtctggc agagattaag tgttcagctt gactggcaat gcgataatgt gcgagcttcc
     4261 tatgatgaaa tcccaaaagc cgtacaagat gaagtattcg caggagcaaa agcctttgtg
     4321 aaagaaaaca aaggacgtta tgactgtggc ggatatatct attctggcga gggtcaagag
     4381 ttagggcaat tctgggcgaa gttagctgtc ggaaatgcca aacttcaaaa gacttccacc
     4441 aatgccaata ttacagacgg caacgggatt tattcaatcg ctggtgcaac atacggtgtg
     4501 tattctgata aagactgcac gaaacagctt gccaccctta cgactgatac cagcggaaat
     4561 acagaagccg ttgaagtaag agccactaca gtatatatca aggaactttc tgcaccagca
     4621 ggatttaaaa ttgataaaac agtttactct ttatctgtgg aagctggaaa aacagcgact
     4681 ttaaaagtat ctgatacacc aaaagtaaca gataccttga ttgagctatt caagattgac
     4741 atggaaacac agaaatccaa ttcgcaggga aacgcttctt tagagggagc agagtttaca
     4801 tggaacttct acgctggata ttacaacaag aacaatcttc cagcacagcc tacaagaaca
     4861 tgggtaacaa agactatcgc tgaaaaagac agcgacggag ctatccatta cattacccgt
     4921 ttagctaacg cttataaagt atctggagac agcttctata tgcaggacgg taaaaacgtg
     4981 cttcctttag gaacactaac cgtcgaagaa acaaaaagtc cgtctggtta cttattagag
     5041 ggtgcatata tgcaggcaga cggtagcgaa gaacagataa aagaaatgta tctgacacag
     5101 attacagagg acggcgacct tgccgtattg tctggaagca atcaatacca tgtttctgat
     5161 aaagtcattc gtggcggtgt aaaaatccag aaacgagatt tagaaaccaa agacacgaaa
     5221 gctcaaggcg gtgcgacttt gaaaaatacc gcctttgaaa tcatttcctt aaatgagaat
     5281 gccgtcttag ttgagagcaa gttatacaag aaaaatgaag tgttaaagac aatccatacc
     5341 gacattgagg gtatcgcttc tacttctgct gacctattac cttacggaaa atacagattg
     5401 tctgaacaaa aaccgccaga gggatatttg actgacggtg caaagccgat tgattttgaa
     5461 atcacagaga atggaaaaat cgttgatttg actgacgaag cccactctat ctataaccag
     5521 attaaacgtg gcgatattga gggcgtaaaa attggtgcag gttcgcacaa gcgtcttgca
     5581 gatgttccgt ttaggatcac aagcaagacg acgggcgaaa gtcatgttgt tgtaactgat
     5641 gataacggac aattctctac tgcttctgat tgggcttccc acaagcacaa taccaatgca
     5701 ggaaaaacaa gcgaggacgg tgtgtggttt ggtacttctg aaccagacga cagcaaaggt
     5761 gctttgattt atgataccta catcattgaa gaattacgtt gtgaaagcaa caaaggcttc
     5821 aaacttattc caccgtttga gattgtcatt tccagaaata aggttgtggt tgatttaggt
     5881 acgctgactg atgaatacga aaaagaaatc actatccata ctactgctac cagcaaggac
     5941 ggcgaaaaga ctatccttgc agggaatgac gttactatca ttgatacggt caaattagac
     6001 ggtcttgtaa aaggtacaaa gtatcagtta aaaggctggc agatgttgaa aaaagaaaat
     6061 gccgagcttc ttgtaggtga taaacgagtg gaaagtgatt atacctttgt cgctgatgat
     6121 gaagctatga aagtggaaat cgcttataca ttcaatgcgt ctgctcttgg cggtaagaac
     6181 cttgtaactt ttgaagaatt gtatgattta agcaatccag aagaacccgt gaaagttgcg
     6241 gaacataaag atattgagga cgacggacaa acggtactta tcacagaacg tatcatcaaa
     6301 atccatacca cagctacgga caaggacggc aagaaagaga ttgaagctgg caaggacgta
     6361 acgattgttg atacggtcaa gttagagggc ttagaagtcg gtacaaagta tcagcttgtg
     6421 ggctggcaga tgttaaaaga agaaaatgca gagcttatca tcaatgataa aaaagttgaa
     6481 aatgactaca catttacgtc tgacagtgaa actatggaag taaagattgc atttactttt
     6541 gacgcttctt ctcttggcgg taaacagttt gtaacttttg aggaattata cgatttaagc
     6601 aatccagaca aaccgataaa agttacggag cataaggaca ttgaggacga cgggcagacg
     6661 gttactatta aggaagtacc agaaacacct acgccagaag aaccagagaa gccaactaca
     6721 ccagatacac caacgaaaac agacagtcca aaaacgggcg acaatacaaa tattcttgcc
     6781 tttgcgatta tgatgtttgt ttctgctggc ggtctggctg gtacatactt cttcaaacgc
     6841 cgtaagatga agaaatcata aggcggtaac tgcatgagaa aagaaaaatc acgctctccg
     6901 ccgtaactta aataggcaga ctacttctat tctggcttgt ctgccgatta cctgcaagaa
     6961 cccagatcgt caagagtgcg tcagcataca gtttaccctt tactgtctgg gcttgctggt
     7021 atctcattaa ctgccagaat acaaaacaat caaactaata gaaagaaacg aggtaaaaca
     7081 atatggaatt aaaatatatc gtgccgaatg tggcacagac ttttggaaac ttggaatttg
     7141 caggggaaaa caaagtagac cagcaacata tcaatgggcg ttacactccc gtatcaagaa
     7201 gttacaactt gtattcggat attcagagag ccgacgacat tatcgttgta ttgcctgcaa
     7261 aagctggcga gaagcatttt aagccagagc agaaagtaaa actgattaac ccacgcatta
     7321 ccgcagaggg ttacaacgtc aaaggacgtg gatttacaaa ctacatttta atggctgatg
     7381 atatgttacc agcagaaagc aaataagggg gtatttgatt atgagattag caaatggaat
     7441 tgtattagat aacgaaaaaa cttttggagt attgaaattc tctgcacttc gacatgaggt
     7501 gcgtgtagaa aatgaggacg gaacgacaag tgatgaaatt aagcgtcgta cttatgattt
     7561 gaaatgtagt gtgcaggaac gcatgataca agtgtcaatc cccgctgatg ttcccgtaaa
     7621 ggactttcct tataatgcgg aagtggagtt aatcaatccc gtagctgata cggtggcaaa
     7681 tgccaacttt agaggggctg atgtggactg gtacatcaag gctgacgata ttatcttgaa
     7741 gaacaaaggc aaccagccag caggaaatcc acagaacaac aatccacaga accttgcacc
     7801 acaaggacaa caaaaaaata aactggcaaa ctagattttc attgtagcaa atataagtga
     7861 ctgttataat atgggtatgg aaacagataa tttgaacttg cggaggtagc gaaaatgaaa
     7921 tttaaaaatc ctatgttggt agtgactgat atagataaat cagttgagtt ttataaaaaa
     7981 gtttttggac ttcgtgtaat tatggatttt ggagcaaata aaactttaac aggtggctta
     8041 gccttacaga caagtgaaac atacaaagag tttatcggta caagtgatat ttcttttggc
     8101 ggtaaaaatt ttgaagtcta ttttgaagaa gatgattttg ataaatttgc tgataggctg
     8161 aaagaatatg atattgaata tgtccaccct attatagaac attcatgggg acaacgtgta
     8221 gtccgctttt atgacccaga taaacatatt atcgaagttg gagaaaacat gaaaatagta
     8281 tgcaagcgtt tcttaaatag cggaatgaca ccagaacaag ttgcagaacg catggacgtg
     8341 cctatgaaat ttataaatgc ctgtatgaga taaattcttg tttgtagaag tcaataaata
     8401 caaagataaa taagaagtta taaaggagaa atgagtatgc tggataaaat acctaatgca
     8461 gaagaaatga ctgctttagt cgggcaatca ttatatgcta tatggaataa actgtgtgcc
     8521 ttaattgatg aaaagtatga catggaatgt ttatggaaca aaggtggtaa agcgtggact
     8581 tatgagtata aatatcgtcg tggcggaaaa acgctttgtg cattgtacgc aagggaaaac
     8641 tgtgtaggat ttatgattat cttagggaaa gatgaacgat taaaatttga tacagataga
     8701 aatagttatt caaaagaagt gcaaaaaatt tatgatgaaa caaaaaccta tcatgacgga
     8761 aaatggttga tgtttgagcc aacagataca atcttattta atgattttgt taaattattg
     8821 ggaataaaaa gaaagcccaa caggaaatga tgattttata cttcaattta gtatacaaat
     8881 cggaagttga ggaggacaac tattatggat aaagtagtta tttatataca tggtaaaggt
     8941 ggaaatgcag aggagtcttc acattacaaa cctcttttta gtgattgtga tgtggttgga
     9001 ctcaactata ctgcacaata tccatgggaa gcaaaagaag aatttccatc actttttgat
     9061 ttgatttgca aacattacaa atctgttcaa atagttgcaa atagcatagg tgcctatttc
     9121 gccatgaacg ccttatcaga taagcaaata gaaaaagcat attttatttc acctattgtg
     9181 aatatggaaa aactgattgc agatatgatg tattgggcaa atgttacaga agatgaactt
     9241 cgagataaaa aagaaattaa aacaactttt ggagaaacac tctcatggga atatctctgt
     9301 tatgtaaaag aaaatccaac tacatggaaa attccaacaa atattttatt tggtgagaaa
     9361 gataacttga cttcttatgc aactatatct gaatttacaa aacaaactaa tgcaacactt
     9421 actgttatga gaaacggaga gcattggttt cacacggaag aacaaatgaa atttcttgat
     9481 gagtggataa acaagtctgc cagataaatc ccagtttgta aaagtcaata aatacaaaga
     9541 taaatttgaa gttaggagac tggtactatg aaaggatttg aacgaaaaaa ccaattattt
     9601 tctctttgcg gattgaattg cggactatgc cctatgcttt tgggaaatta ctgtggcggt
     9661 tgtggcaatg gtaatcaatc atgcagaatt gcaaaatgta gtcttgaaca tggacagatt
     9721 gaatattgct atgaatgcaa acaatatcca tgcgaaaaat atcaacacat tgatgattat
     9781 gattctttca tcacgcataa acagcgaaaa gcagatttag aaagagcaaa aaacataggc
     9841 attgagcaat ataatcttga acagcaggaa aaaacacaaa ttctttctta cttactttct
     9901 aactataatg acgggcgtag aaagactttc ttttgtgtgg cagtcaattt attagaactt
     9961 tcagaattac aagaagcaat gaaacaaata caatccaatg acgaattgac cttactacca
    10021 tttaaggcac aatgcttgta tgtggtggag gtgtttcaga agattgctga aaggagaaat
    10081 ataaaactga aacttttcaa gaaaaagtga ttttcaaatt tgaaattgta gcaaagagtt
    10141 gtaactgcat tgggaagcta tttatatatc ctaatgccta tactgaaatt caaacaaact
    10201 gcaaaatatc atgaggaata tacgctggta ttttctaagg aaaacattaa atttaaaaca
    10261 caaagcattg actcagaagt gaagtgggac atttactctg cactatggga aagccatgat
    10321 ttttattact taattcaagc accacgatta tatacgttaa ttcccaaaag agtttttaaa
    10381 gacctaaacg aaaaacagct atttgaagaa attgcacaat caagagttaa aacaacaaag
    10441 catatataat gaacataccc tacaatgtaa caacttcata ttcatacaca caaagcggtt
    10501 agtcttttaa gattgactgc ttttttcata cttaaattta agattggagt gattgattta
    10561 tgcgaataat ttggaataaa ggacaccgta tcagagccag cgataagaac ctcgtctatc
    10621 gcttttcatt gggaacgctt ctaattgtat tcgtggtggt tcttctgcta ctgaatatga
    10681 aacagctcat gcgtaccgac tgggaacatt tcagcttatt agacaacagc tttacgcttt
    10741 ccttatacaa cttcataacc ttactgatag cgactggtgt ttgtgcattg gtcgcttttc
    10801 tgtattaccg cttttgttat gacaatttta aaaagctact gcaccgtcaa aagctggcac
    10861 gaatgatact ggaaaataag tggtatgaag ccgataccgt acaagacagc ggttttttta
    10921 ctgacctgca aagcagatca agggaaaaaa tcgtctggtt tccaaagatt tattatcaaa
    10981 tggaaaaggg actgcttcat atccgctgtg aaatcacatt aggaaaatat caagaccaac
    11041 ttttacggtt agaggataaa ttggaaagtg gcttgtattg cgagctgacc gacaagacct
    11101 tacatgacgg ctatattaaa tatatcctgc tttatgacat gatagcaaac cgcattacta
    11161 ttgatgaagt acgggcagaa aacggctgtc ttagactgat gaaaaatctt gtctgggaat
    11221 atgacgcact ccctcacgct ctgattgctg gtgggactgg tggcggtaaa acctattttc
    11281 ttctgacgct cattgaagcc ctactgcata ccaacgctgt cctttacatc ttagacccaa
    11341 agaacgctga ccttgcagat ttgggaacag tcatgggaaa cgtgtatcac actaaagaag
    11401 aaatgattga ttgcgtcaat gccttttatg agggcatggt acaacgaagc gaagaaatga
    11461 agcaacaccc gaactataaa acaggagaaa actacgccta tcttagctta ccgccctgct
    11521 ttttaatctt tgatgaatat gtggcgtttt ttgaaatgct ggggacaaag gaaagcgtga
    11581 gtttacttag ccagttaaag aaaatcgtca tgctggggag acaagcaggg tatttcctta
    11641 tcgttgcctg ccagcgacca gacgcaaaat atttctcgga cggtatcaga gataacttca
    11701 atttccgtgt agggttggga cgtatcagcg aattaggtta cggtatgctg tttggttctg
    11761 atgtgaaaaa acagtttttc cagaagcgta tcaaagggcg tggctattgt gatgtgggaa
    11821 caagcatcat aagtgaattt tacacgcctt tagtcccgaa aggatatgat tttttgcaga
    11881 ccataggttc tcttgcatat gcaaggcagg acgggacggc gacgtgcgaa gcgaaaggcg
    11941 acggcacgga ctagccgttg ctggtgtggc taacgccaca ccagcagaaa caagcccctc
    12001 attatctaac agaggggcac aattacggcg gaaacttcca caaaaacctt tgcaagaccg
    12061 catggaatga gggtttttcg tacatctcgc cttttgtgat aaaaccttga aattatcaca
    12121 atatcacaaa attatcacga ttgggggtat gacaactgaa tgaaactgat tttttaaccg
    12181 ccttgaaagc gaaacgctgt gcatacagcg tatcgcagac aaggcttgca atcatggcaa
    12241 atatcagccg tgaacatttg agccgtatcg aagcaggaaa ggtaacgctg actgaggaca
    12301 tgaaacatac gcttttagaa gcggtggaaa aattcaatcc cgacaatccc atgttcctgc
    12361 tgtttgatta tgtgcgtatc cgttttccca caatggatat aaagcatatc atcaagaata
    12421 tattaaagct caatatcgac tatatgcttc acgaagatta cggacattat aaatacaccg
    12481 agcattatca tatcggagat gtgtttgtgt atgtatcgca ggacgaggaa aaaggtgtcc
    12541 tgctagaact caaaggcaaa ggctgtcgtc agtttgaaag ttatctgctg gcacaagagc
    12601 gaaattggta tgacttcttc acggacgcat tggttgaggg cggtgtgatg aaacgtcttg
    12661 atttggcaat caatgaccga acgggtttac ttgatattcc agagctgata caaaaatgcg
    12721 agaatgagga atgtatttct aaattccgtt ctttcaaaag ctatggttct ggggaactgg
    12781 taaagcataa cgaaacggat aaatacggta tgggacacac gttgtatatc ggttcatttt
    12841 caagtgaagt atacttctgc tgttatgaaa agaattacga acagtacgca aaacttggca
    12901 taccgattga agaagtcccc ataaaaaata gatttgagat acgtttgaaa aatgaacgtg
    12961 cctattatgc tgttgtggaa ctgctgacac attatgacgc agaacagaca gcattttcta
    13021 ttatcaacca gtatatccgc tttgctgata aagaaccaga caagcgaaaa agcgactgga
    13081 aaacaaatgt acgctgggct tggttcattg gcgaggggcg accacccgta aagctgacaa
    13141 caaagccaaa accgtacacg ttagaacgta cactcaactg gctacaaagg caggttgcac
    13201 cgaccttgaa gatgttgaaa cagatagaca aggacaatca gacggagtat ctggaaacga
    13261 ttgagaagca ggcaaaactg accgagcgac accaccagat aatcaagcaa cagacagcca
    13321 cggttgaaga aatggttgcc aaagtaaagg agtgatgaaa aatgtgggaa ttattgaaag
    13381 acttcctgct ggtatctctg ggaatgggta tcggcgtagt cttgatgtgc attttaactg
    13441 tcggcaaaga agctgacaga tgtatggaag aaatcaaaga aagagaggaa cattaaatga
    13501 attttggaca aaatttatgt gcgactcgtt ccttagtgaa aagctaaggc actaataaaa
    13561 gaacgaaaaa gtttgattat taggagaact gataatctga caggtggaat gatgaagtaa
    13621 cgctctgaaa cgcctgccct gagcctccga caagcattga atgaggaaac tcagaatgtt
    13681 tgaagctcgg taaagacggc tgaaagatac cgtaatagtt gatgaacata tatcttctat
    13741 cgaaaactat ccagaggtgg gtagcgtatc caacaggtcg ggggtctata aaatatctat
    13801 ggttagaatg ttccacaagg gactgacgaa acagcgaatg tacgggtcta aatttgtact
    13861 ttgtagaaat gcaaaggtac gagaaatcgt agctggtgtg gcaaagtaag aatataggtt
    13921 atgaaatgcc atataccgtt acaggcggta tcaagccagc aggcttatag ctagtgacct
    13981 aaggatatat atgtatagat aagattatcg gaacgaggaa aggtattggg ttctcatcaa
    14041 gagaataagc tgacgaagaa caataagcag ttgaaccaat gctgaaaagc agagggcgaa
    14101 cctaagaaat tcttgtaatg agaatggagg aataccctca agtcgtgtat atattagaaa
    14161 agtattatgt tgttttaata aggattacga gtaagataaa aaagctcact caatttcaag
    14221 caaaggagtg aagccttatg cctaaaaata aaaatgaaac attgtgtgta gaagatttaa
    14281 gacacgctga atattacgaa atgcaaaaca cctttgatga tttatatgcc aaaagtaaga
    14341 atggagatat tttcactcat cttatggata ttatcctatc gagagaaaat atattactgg
    14401 catatcgaaa tatcaaagca aatgcaggca gtaagacagc aggaacagac ggtacaataa
    14461 ttaaagatat tggcaaattg ccagcagaaa cagttgtcaa aaaggtaaga tatattgtcg
    14521 caggcactcc acacgggtat cgacctaagc cagtaaggcg aaaagaaata ccaaaaccaa
    14581 atgggaaaac cagacctcta ggcattcctt gtatgtggga caggcttata caacaatgta
    14641 tcaaacaagt attagaacca atctgcgaag ctaaatttag tgaaaacagc tacggtttca
    14701 gacctaatag gtcggttgaa aatgcaataa aagcaaccta taaccgccta caaatcagcc
    14761 agctacatta tgtgattgaa tttgatatta agggtttctt tgacaacgtg aaccattcca
    14821 aactgataaa acagatatgg gctatgggaa tacgggacaa gcacttgata ttcattttaa
    14881 aaaggatatt aaaagctcca attaaaatgc ctgacggcac aataacatat ccagagaaag
    14941 gaactccaca ggggggaatt atatccccac tactagccaa cattgttctt aatgagttag
    15001 accattgggt agaaagtcaa tggcatgaaa atcctgttac aaaaaactat gtggttcata
    15061 tcaataaaag cggtagccca tgtaaaagca acgcttacaa agaaatgaag aaaacaaagt
    15121 taaaggaaat gtatatggtt cgttatgcag atgattttag agttttctgt cgctacaaag
    15181 aaagtgctga aaaaaccaaa atagcaatta ctcaatggat cgagcaacga ctaaaattgg
    15241 aagtatcaca agaaaaaaca agaattgtaa atgttagaaa aagatactcc gactttcttg
    15301 gtttcaagat taaaatgata ccgagaagaa agaagttagt tgttaaatca catataagtg
    15361 ataaacaatt caagacccaa aaagacaagc tggtatcaca ggcaaagaaa attgcttctc
    15421 cctcaaaggg aaaaacagaa ttagaggaaa taagacttta taattcaatg gtattaggta
    15481 tgcaaaatta ttatcagatt gcaacaaatg taaatctgga ttgtatgaaa cttaatagag
    15541 ctgtaatggc tatcttcact aacagattag gtgcaagcaa aaagggcaga ttgagaaaaa
    15601 caggcagaca acttacaaca aaagaagcaa caagatatgg taaatctaaa atgcttcgat
    15661 atgttgctgg cgagggcgaa cctatattcc ctgttggata tatccaacac agaaaaccaa
    15721 tggcaaaagt gtattcagca aactgctata ctccagaggg aagaaaattt atccatacca
    15781 atctaagtgt tgataaatta ctgatgtatc agttgatgaa attatctttg gaaaatcgca
    15841 caatagaata cgcagacaat cgaatttcgc tattttctgc ccaacatgga aaatgtgcta
    15901 taactctgga agaatttcaa caagctaatg aaatacactg tcatcatatt gtaccaacta
    15961 gggctggtgg taatgacgat tataaaaatc ttattttggt aaaagaggcg gtacatcgtc
    16021 taatacacgc aaaaacagaa gaaacaatac aaaaatatat agctctattg aaactaaata
    16081 aagtccagct cataagacta aataaatata gagtattagc tggcaatcaa gaattaaact
    16141 tgatataggt ctactacata aatacttttc aatataaaca cgatggaacg cggagtgcgg
    16201 tgaaagtcgc acgctccgtg tgaatggggg gaaaagtttg agatatttat aatatcagga
    16261 acttacctat ccatatacac ttggttttta agcaacgcac agagtttagt gcttatggcg
    16321 attgtggtaa ttggtatcta cttaggtttt aagcgtgagt tttcaaaact tatcggcttc
    16381 ttggtagttg ctcttgttgc cgttggcttg gtattcaacg ctggtggtgt gaaagacgta
    16441 ttgttagaac tgttcaataa gattatcggt gcataaaatt ttattgtagt gtgagtgtag
    16501 aaatggtatg atatggatat gaagaaagca acaaattaga agttggagga acaataatgg
    16561 actacaaagt agatgataaa aaccttgatg catcaatgtt tatttctttt gtcaatcaag
    16621 tatggcaggg cgattatgat atggaacaaa caaggcaagc attatctaaa acactaaaca
    16681 taacagccta tgacaataaa aagttagtag ggtgtttgcg tattctttcc gatggttact
    16741 attttggaac aattacagag ctacttgttc ttcctcaata tcaaaaacag ggtattggaa
    16801 gtaaacttct tcaacttgca aaagacaata cacctacaat gttatatttc ggttctcaac
    16861 cagaagcaga gaagttttat gaaaagaacg gctgtcaaaa aagcttacaa tcttacatga
    16921 taaataaggg ataataagag ataacttcca gcttgacaat tttatatcca tatacacaaa
    16981 gcagttagtc ttaggattga ctgctttttt cttacctaaa aattagattg gagtgatgta
    17041 atgaacgata tttttaagaa tatgcaggca aaaatcggct gtgaatatct ttctgacctg
    17101 ccctcttaca aacgtaaggt gtggcatgaa atgaaacaac tgaaccttgc cgattatgaa
    17161 gagaaacagt tagaagattt ttcaaagtat gtgtttggta tgtcatacca gaccttaaaa
    17221 gatgtgatga aacaacagat aggacgtgag gaacaatgca ggaaacaagg gtactggtgg
    17281 aaacgaaaag aacaactgac gaagaaacaa catcatactg gttcgacctg ccgatagagg
    17341 ttgccgaatt tgaggaaaaa ttaggtgtcg gtgcagaaag tggggaatac cgtattatcg
    17401 aaaaggtatt gccttatgct gatgaagtcc acgaacatac aagcgtgtac cagctcaaca
    17461 aattagattt tatgtaccgc cagctttcaa gcgatatgca ggaagaatat gtatcactac
    17521 ttatggtgtt tgagaattta gaagcacttt atatttgcag aaatgtaatt accgtttatc
    17581 ccgactgtaa aagtatgata gatgttgcaa ggcaaaagct gatgaacgac ccgacgttca
    17641 aacatttatc tgaggactgt caagaatact atttcaattt tgaagcctac gcttctcact
    17701 tgcaggaaca cgggaaattt ttagtaacag aacacggtat ctttgaactg ccagagtagg
    17761 aagtgaggtg gggcttatga ttgatgatat ggcggtttat attgctaatc ttggcaaata
    17821 caatgaggga tatttagtcg gtgcgtggtt ctctttccct attgacgagg aagatgtgaa
    17881 agaaaaaatc ggtttgaatg aacaatatga ggaatacgct gtccatgata ctgacaactt
    17941 ccctatcgac ataggggaat atgtttccat tgaagaactc aatgagatgt atgaaatgat
    18001 agaggaactt cccgactata tcgtagagtg tctggacgaa tttatcagcc actacgggac
    18061 gctggaagaa gtcgtggaac acaaggacga tatttattat tatccagact gtgaaaccat
    18121 gacagacgtt gcctactact acatagacga attgcaggca cttggcgaca ttccacccag
    18181 cttacagaac tacattgact atgaagccta cggtcgagat ttggatatgg gcggttgctt
    18241 tattgaaaca agccgaggta tgtgcgagat accttattaa cgctggaaga tcagcgacaa
    18301 gcattgttgc tactgacagc gtatttctct tttaagggac agtctgaaac atggctgtcc
    18361 ttttctcttt ttaagaactt acgaaaggag ctgaaagaac ttgaaaaaga ttaaaagcta
    18421 cacgggtatc tggaacgtgg aaaaagtctt gtatgcaatc aatgacttta acttaccctt
    18481 tcccgttacc tttacacaga ttacatggtt tgtaattaca gaatttatca tcattctgtt
    18541 tggggatatg cccccacttt ccatgattga gggagcattt ctcaaatact ttggtattcc
    18601 cgttgctctc acttggttta tgtcgcagaa aacctttgac ggaaagaagc cgtacagctt
    18661 tttgaaatca cagataacct atgccctgcg acctaaaatc acttatgcag gaaaagccgt
    18721 aaaactgcat aagcagacct tgaatgaaac aatcacggca gtaaggagtg tgaactatgt
    18781 tcccgataaa atatattgac aataaccttg tctggaacaa ggacaatgag gtgttcgcct
    18841 actatgagct gataccatac aattattctt tcctatccgc agagcagaaa tttatcgtgc
    18901 atgacagctt tcgacagcta atcgcacagt cccgtgaggg taaaattcat gcgttgcaga
    18961 ttgccacaga aagctcaata cgaagtatgc aggagcagtc aaagaaactg gtaacgggga
    19021 aacttcgtga ggtggcagtc caaaaaatag acgaacagac cgaagcgtta gtatctatga
    19081 ttggggacaa tcaagtggac taccgctttt ttcttggctt taagctcatg gttacggaag
    19141 aacaactcaa tctgaagaat attaaaaaat cggcatggct gacgttcacg gaatttctcc
    19201 atgaagtgaa ccacacgctg atgaatgatt ttgtttccat gccgaatgat gaaatcaacc
    19261 gttacatgaa aatggaaaag ttactggaaa ataaaatctc ccgtcgcttt aaggtgcgtc
    19321 gcttggaaat caatgatttt gggtatctca tggaacatct ttacggcagg gacggtatcg
    19381 cctatgaaga ttatgagtac cagctaccag agaagaaatt gcagaaagaa acgctgataa
    19441 aatactacga ccttatccgc ccgacaaggt gtgtgattga ggaaagccag cggtatttac
    19501 gattggaaca tgaggacaag gaaagctatg tgtcttactt tactgtcaat gcgattgtag
    19561 gcgagcttga ttttccgtcg tctgaaatct tctatttcca gcaacagcag tttacattcc
    19621 ccgttgatac ttctatgaat gtagaaatcg tggagaacag aaaggctctt agcacagtaa
    19681 gaaataagaa aaaggaattg aaagaccttg acaatcacgc ttatcaagca ggaagtgaaa
    19741 ccagctcaaa tgtggtagac gctttagaca gcgtggacga gctggaaaca gacttagacc
    19801 agagcaaaga aagtatgtat aagttaagct acgtgatacg ggtgtctgca cccgatcttg
    19861 acgagctgaa acgccgttgt gatgaagtca aggactttta cgacgacctc aacgtaaagc
    19921 tggtgcgtcc tgctggggat atgctggggc ttcattctga atttttacct gccagcaagc
    19981 gatatatcaa tgactatgtg cagtatgtaa aatcagactt cttggcaggg cttgggtttg
    20041 gtgctaccca gcagttaggg gaaaatacgg gtatctatat gggctattcc gttgatacgg
    20101 gaagaaatgt gtacctgcaa ccgtcccttg ccagtcaagg tgtaaaaggt acagttacca
    20161 acgcccttgc gtctgctttt gtcggttcgc ttggcggtgg aaagtcgttc tgcaacaatc
    20221 ttcttgtgta ttattcggtt ctctttgggg gacaagcggt tatcttagac ccaaaatcag
    20281 agcgtggcaa ctggaaagaa acacttccag aaatcgcaca tgaaatcaat atcgtaaatc
    20341 ttaccagcga caaggacaat gcaggacttc ttgacccatt cgtgattatg aagaatgtaa
    20401 aagacgctga aagtctggca atcgatatct taacattcct tactggtatt tcctctaggg
    20461 acggcgaaaa attccccgta cttcgtaaag cggtacgctc cgttacccag agcgacagtc
    20521 ggggcttgct ccatgtgata gacgagctac gccgtgaaga tacgcccata tcaagaaata
    20581 tcgcagacca tatcgacagc ttcacggact acgactttgc acacctgctg ttttcagacg
    20641 gtacggtaga aaatgctatc agtctggata accagctcaa tatcattcaa gtagccgacc
    20701 ttgtgttgcc agataaggac acgacctttg aggaatacac gaccattgaa ttgttgtctg
    20761 tgtctatgct gattgtgatt agtacctttg cccttgattt tatccattcg gacagaagca
    20821 tttttaagat tgtagatttg gacgaagcat gggcgttctt aaatgtggca caaggcgaaa
    20881 ccttatcaaa taagctggtt cgtgctggac gagctatgca ggcaggcgtc tattttgtta
    20941 cccagtctgc ctatgatgtt tcaaaggaaa gtctgaaaaa taatatcggc ttgaaatttg
    21001 cctttagaag taccgatata aacgagatta aacagacctt agaatttttc ggtatcgaca
    21061 aagaggacga aaacaaccag aaaaggcttc gtgatttgga gaacggacaa tgcttgttgc
    21121 aggacttata cgggcgtgtc ggcgtagtac agatacaccc agtctttgaa gaactgctac
    21181 acgcctttga taccagaccg cccgtacaga gaaatgaggt ggagtgatga aagaaaggat
    21241 acagagtgcg ttcacaaaaa agaagatgtt ccgctttctc aagataacgg tgtttgtcgt
    21301 ggcactttcc cttatcctgc tttcactact tgaaacggtg gctcatgcaa cggggcttgt
    21361 ggacgatacg ataaatacag aaaatttata ttctaaatat ccgttatcca actatcagct
    21421 tgatttttat gtggataata gctgggcttg gttgccgtgg aactggctgg acggtatcgg
    21481 aaaatcggta cagtacgggc tttactgtat tactaacttc gtttggacta tcagtttata
    21541 tttaagcaat gccacgggct atgtggtgca ggaagcctat aagctggact tcatcaatga
    21601 tatggcagac agtatcggac agagcataca gacccttgca ggcgttacct caagcggttt
    21661 ttcaacctct ggcttctatg tcggtttcct gcttctcatt atcttggtgg tgggaattta
    21721 tgtagcatat acgggactga ttaaaagaga aaccagcaag gcacttcacg ccgttatcaa
    21781 ctttgttgtg gtgtttattt tgtcggcttc atttattgcc tatgctcccg actacatcaa
    21841 aaagataaat gaattttcaa gcgacatcag taccgcttct ttggacttgg gaacaaaaat
    21901 catgctccca aactctgaca gcgagggcaa ggacagcgtg gacttgataa gggacagcct
    21961 attctctatt caagtggaac agccgtggct actcttacaa tttgggaaca gcaacgcaga
    22021 ggaaatcggg acagaccgtg tggaagctct ggtatcggta agcccagagg acgaggacgg
    22081 aaaaaccaga gaggaaatcg tgaaaacaga aatcgaggat aacgacaaca acattctgac
    22141 gataccgcag gtagtaaacc gtttaggtat ggtgtttttc ctcttattct tcaacttagg
    22201 tatcacgata tttgtattct tgcttacggg catgatgttg ttcagccaga tactttttat
    22261 tatctttgca atgttcctgc ctattagttt cctgctctct atgataccga gctatgaaag
    22321 catggcaaag caggcaatcg tgagggtatt taataccata atgacacggg caggaataac
    22381 gctcattgta acggtggcgt tcagcatttc cagtatgttt tacaatatct ccacagacta
    22441 cccgttcttt atggtggcgt tcttgcagat agtttgtttt gcaggtatct atatgaagct
    22501 gggcgattta atgagtatgt tctcacttaa tgccagcgac agtcaaagca tgggacgcag
    22561 gatattccgc agaccgtatc tgtttatacg acatagagcg agaagaatgg agcgacgtat
    22621 ggcaagggcg gtaagtgctg gcggtatttc gggcggtgtc gctggtgcgc tggctggcag
    22681 taccgtttct aatgcaagga caacaagaaa caatcctaca aaggaaaatc ggggcaatac
    22741 cacttccatc atgggacagc gtgcaggctc aaagataggc gctgtcttag atacgaaaaa
    22801 taaagtgaaa gacaaggcaa atgctgtcaa agagaatgtt aaggatatgc cgacacagac
    22861 cgcttatgcg gtgtattctg caaaagaaaa ggcaaagtcc agcgtgtccg acttcaagcg
    22921 tggcatggtg caggaacagc aatccagaca gacgggacgc ttagaaaagc aggaacaacg
    22981 taaaaaaaat atcgctaaca agcgtatgga gcttcaaaag gcacaagaag caaggcaggc
    23041 acgaaaggct gacggatcag cgacaacggg agctacccgt ccccatgagc gaccagccac
    23101 agcttcaaag ccgagtgtgg aaaaagtgca ggaagtcaaa cgccctgcca caactccgac
    23161 ttcaaaagca agtgagccag tcaagacaaa tgttatcaaa gagcgtccgc tatcttcggg
    23221 tgcttctgac ggaaaagcaa cccagtcggc acagccaata cataggcaga atatagaaaa
    23281 agtggtatcg caggaaacac gccagaatta cgttaaagac cgcaggacaa aggttcagca
    23341 gacgcagagc gtccagaaga accagcagac cacagagaaa aaccataacc ttgtaacgaa
    23401 gaaaggacag aagaaaaaat gaaactgaaa catatcgctc ttatcggcag tctgtttcct
    23461 atcctctttt ccatagtcct tttttttggg gtgctgatta gtgcggacag cgacgacgaa
    23521 aacagcaact tttcttctgg tattacgggt ttgaacctat ccgcagaagt cttgaaacat
    23581 cagcctatgg tggaaaagta cgccagagaa tacggcattt ccgagtatgt taatgtatta
    23641 ctggctatca ttcaagtaga aagtggcggt acagcggaag atgtcatgca gtccagcgaa
    23701 agtctgggac taccgcccaa ctctttagat acggaaagct caatcaagca ggggtgtaaa
    23761 tattttgcgt ccctgctttc ttcctacaaa aatcaaggaa taaatgactt gaatgtggcg
    23821 atacagtcct ataactatgg cggtggctat gtgggatatg tggcaggaaa agggaaaaag
    23881 catacgttta ctcttgcaga gagcttcgcc tgtgaaaaat cgggtggaaa gaaagtaacc
    23941 tacaccaaca cgatagccgt tgcgaagaat gggggctggc ggtatggcta tggaaatatg
    24001 ttttatgtgg aattggtcaa tcaatatctg actgtaccgc aggtatcggg agaactggca
    24061 caaaagataa tgaatgaagc gttgaagtat caaggttgga agtatgtcta tggtggcagt
    24121 aatccgaaca cttcctttga ctgttcggga cttgtgcaat ggtgttatgg aaaagcgggt
    24181 atctctttac cgagaacggc acaagcccag tatgacgcaa cccagcatat ccccttgtcg
    24241 caggcaaagg ctggcgattt ggtgtttttc cattcaacct ataacgctgg ttcgtatgta
    24301 acccatgtcg gtatctatgt cgggaataat cagatgtacc atgcaggcga cccgatagga
    24361 tatgcagacc tcaatagtag ctactggcaa cagcacttaa tcggtgcagg acgagtaaaa
    24421 taaaagaaag gacttagaaa atatgtttaa gaaaagtaag aaacagacag aaaatgtcaa
    24481 agaaaaaaag gtgcgtactg tcaaggtagg cacacataag aaaactgtaa ttgcgttttg
    24541 ggtggtgctt atcgtaagtg taagttttgg ggtatataag aattttaccg ccattgatat
    24601 gcacaccgta catgaaacag aaaccataca attaagactt accgatacta acgggattga
    24661 aaatttcgtg aagaactttg cgaagtccta ttacacatgg aacaacagca aagaagcgat
    24721 tgaagcaagg acgcaggcaa tcagcggtta tctgacaaag gaattgcagg acttgaatgt
    24781 tgataccatt agaaccgata taccgaccag ttccacggtt acagatgtga ttgtttggaa
    24841 tatcgaacag tcaggaacgg acactttttc tgctacctac gaagtagatc agcagataaa
    24901 agagggagaa cagacaacag cagtcaaggc aatctatacg gtaaaagtcc atgtggacgt
    24961 tgacggggat atggtaatca tacagaaccc tacgcttgca ccaacaatcg aaaaatcgga
    25021 ctatgagcta aagacaccag aagcagacgc aagtgtggac gctgatacca taaatgacgc
    25081 tacttcattt ttggaaacat tctttaagct gtacccaata gcgacggaaa aagagcttgc
    25141 ctattatgta tcgggaaatg tgcttgaacc tatcggcaag gactatcttt attctgaatt
    25201 gataaaccca gtctttacaa aagacgggga caatgtgaaa gtcaaagttg ccgtgaaatt
    25261 ccttgataat caaacaaagg cgacgcaggt atcgcagtat gaacttgtgc tacataagga
    25321 tagtaactgg aaaattgtga aataataatt taaagcgtgc atttcttata agatttgtac
    25381 gctttacttt tatggtaaaa attagccttg acaagcgacc gttggtcgct atataatgaa
    25441 agcgaaacaa gaaacgaccg atggtcgcta taaggaggta tcataaatat gccaaaagga
    25501 attatgctta cacaagaaca acaagaagaa cgccgagaag aaataatcag tgttgctttg
    25561 cagctcatag agaaaaatgg attccaaaaa acatcaatga ggaaaattgc gattttagca
    25621 aacatgggaa agtccagtct gtatgatttt ttcaaaacga aagacgagat ttttgtatat
    25681 gcagttgaga aagaaataga agaaacaatt aaaaaggttc ataggattat tgccgatgaa
    25741 tcctcgccgg aacagtgtct tagaaaaatc atgctgaatc atcttggagt accaaagcag
    25801 tatcgaacgg tgctgatgtg gttaaataca gaatctgatt atttagaaga agattatcga
    25861 aagcggctga aaactgcgcg ttacgcatat caggatatta taaaatctgt aattgaaaat
    25921 ggaatggaat caggcgtctt ccgtaagacg aatgccaatc ttatgacgcg tttgttaatt
    25981 aactccatta tatcaatcat ttacacatcc cgaccatcag ccagtccgga gaaaatgctt
    26041 gatgaaacaa tggatatatt tctacatgga attatgaatg atggaggtga attattatga
    26101 tttattatac tctacccttg ttgcataaac aggcaaccct tgaaatggtc ggcggaaaag
    26161 gtatgtcttt atcaaaactt ttgacaacgg gtattcctgt gccggagggc tttcatgtta
    26221 cgactacttc atacaagatt tttgttgaaa agaaccatat tcagccttac attaataagt
    26281 tgctggacgg tattgacact aacaatacga gccagcttga aaatgtatct acgcagatag
    26341 ggatgctttt ccataatgga gaaatgccgc aggaagtatc agatgcaatt aaaaccgcat
    26401 atgccagatt gggaaatata gcggtggctg tccgttcctc cgcaaccgcc gaggatttgc
    26461 ccgatgcttc ttttgcaggc cagcaggaaa cctatcttaa catacaaggt gagaatgaag
    26521 ttcttgacgc tgtaaagagg tgctgggctt ccctatggac agctcgcgct attgcttacc
    26581 gcgtgaagaa tgatataaag caggaaatcg tcgcacttgc tgttgtagta caaaagcttg
    26641 cgttttccga tgcgtccggc gttatgttta cgctaaaccc tatcaatggc agacgtagcg
    26701 aaatgattgt taacgccgcg tggggacttg gcgaatcggt ggtttctagc ttagtcaccc
    26761 ctgatacaat cgttgtagat aaaaatgctg aacgaattgt atcgtatgaa gtagcaaaca
    26821 aagagattat gacagtacgc aactcagatg gaacagaaga aatcccaact cctgaacagt
    26881 tgaggaaaaa acatgctctt actcgcaatc aagttatgcg attgacacag cttggaaaaa
    26941 aaattgagaa gtattatgaa atgcctatgg atgtagagtg ggcactggaa aaagataagt
    27001 tgtatattgt tcaggctcgc cccattactg tcctaccgcc ggaatggacg ttaccagaaa
    27061 aggatgttat atatacaaga ggcagtctag cggaacactt gccaaatcct gttacgccct
    27121 tattcgccac acttgggctt gaaatagtca accgtgcgtc ggcgctttta tggatagata
    27181 tgtttggtaa aagtgcgaaa aagctactgc cagaaaacgg agcatatacg attattaacg
    27241 gatatgttta tctttccgct aattcaaagc ctcttttgat tgctgtcaaa tccctttcac
    27301 cccgatcttt acgccgtgcg ctcacgaaca gtgttgcacg ctgggaaaca gcacgaaaag
    27361 aatttgagga tgtgattaaa caatgggaag aaaagcccat gcatatgctg aatgctcatc
    27421 aaataatgga ggggattcag actgtatttt atggagcgtg tatttatttc acgaggattc
    27481 agcttacatt gccagccgcc tctatcagcg aaacattatt tacaaaattt tttcaggggg
    27541 cggctcgtcg cgctggtatg acagatactt ctgttttcct gcttggattt gatacgattg
    27601 ctctgcaagc cgaaaagaac ctgtggagtc tctcggaatg ggtaaagcaa aataatactc
    27661 tcaaccttta tctgcaaagt aatccaacag caaagatagc ggaaaatttt atgtcctcag
    27721 ttccgcctga cgaagtttca ctggaagtat ggatagaatg gaaaaatcga atcaatcagt
    27781 attttaaaga gtttggtcgt accgcttatg aatttgattt tgcatattcc acaccgcagg
    27841 aaacacttac accaaccttt gaatcgataa aaacctttgt agaaggcaaa ggtgaaagtc
    27901 cttttttacg tcaatccaaa tttgaaaagc ataggaaaca ggcagaagaa gaaattttac
    27961 aacatatagg tagctcacgc aaaaaactgt ttttgaagtt gctccattgg gcacaggaaa
    28021 ctgctcctat gcgtgaaaat gctatttatt gcatgggaat ggggcatcct ctgattcgcc
    28081 agatgtttca tgaaatttca gcacgcctta tacgtggcgg agctacttcg catatagatg
    28141 atatttattg gctcacaaaa ttggaattgg aaaaactcat agtacagtta gacaaaaata
    28201 tacctttatc cgatagaaga aattcgatac ttgcgcgaaa agcggaactc aaaaagtatc
    28261 agggctatat gccaccagct cagttacctg agaagaaagt aaaaagcata tcacatgcac
    28321 cacagaaaca aaaagatgga aaaaccgtgt tgaggggtat aggaaccagt tctggtgtcg
    28381 tgacagctcg tgcctgcgta ctaaatagtc cggcagattt taaaaatttc cagccaggaa
    28441 gtgttttggt tgccgttact acaactccgg cttggacacc cttatttgct tcagcaagtg
    28501 gaattgtaac ggatattgga ggtcctctca gtcattcaag tattgttgca cgggaatgtg
    28561 gtatccctgc cgttatggcg acacacacag ccacgagaag tattaaaaat ggacaaatga
    28621 taacggtgga cggctcggag gggacggtga cgattgatga ataaaaagcc gattttcgct
    28681 ttaaatcttg ctactttggt gattgcgtta ggatatagct taattcttcc ggtcatgcca
    28741 ttttacatgg aaaatctagg tgctggcggt cgtgagcttg gatggctcac tgccgtatat
    28801 gccctggcac aaacagtatg cgcacctttt tggggtgcac tgtccgaccg aattggtaga
    28861 aagccgatta tcagcattgg tattatagga tattccatca gtttgttcct gttcggtttg
    28921 gcttcatcct tttggacact atttatagct cgcagtttat cgggtatttt gtcatctgct
    28981 acgtccgttg catctttggc atatgttgga gattccagct cggaggaaga aagaagcaag
    29041 aatatgagtc aacttggagc ttcaatgagc gttggcgtaa tgatgggacc attattggga
    29101 ggcattcttg caggctattc gcttgcgctc ccatttttta caggagctgg catttcattt
    29161 attgcgtttc tgctaattct aacacttttg ccagagtcaa ttgagcgttc aggacagacg
    29221 gaaaaaagaa aaccctttga tttttcagac ctgaaaagta taatttttgg ctcagcagga
    29281 atggttttga tacttatttt cgtcgtgcat ttctgtcaaa caggcttgca aggaattacg
    29341 gggctgtatg tcgtagataa gtttggattt tccactaagc aagttggtgt tatttggatg
    29401 gtgctagcag gtattttgat tgtagtacaa ggctttttga ctggtccttt agcaaaaaaa
    29461 attggtgaga agcgactaat actgataggt atgttttgca aagccttagt agcgtttgcc
    29521 atggtattga caactgggtt tgttagtgtg ttggtggttt ttggcttatt tgcggtatct
    29581 tctgctatta cagtacccac attaaacgca accctttcaa aaactgacaa tcagcataaa
    29641 ggaacactga tgggatttgc cagtaccgcc ggaaatctta gcaaagttat tgctccttta
    29701 ttaactggtt atctgtatga aagcaatatt gaattaccat atataaccac gggagggata
    29761 gccttaatcg gagtggttat ctgctatatc tggataaaaa atcaaaagaa caaatgatgg
    29821 aagaggacgt ttatatggag gttatacaca gtaactaatc tggtatagaa attcaattga
    29881 ggcttttctg ctttattaaa gtcttgctac tacttaaacc tataatatcc ttaattcatt
    29941 atgacaaatt ccaattcaac agttttgata cacataagga ggtaacaata ctatggaaaa
    30001 ttatcaatga agtgctaatt ctttatcagt gaagttatcc ataagttttt ctacctgtaa
    30061 tcgtctggtg ctttttgcca gatcattggt aggtaagaaa aattcatcaa cagacacatg
    30121 aagtaatgat acaaggtcac ttgtatgctt ggatgttgcc ctttattttc aatattagtt
    30181 aagtaacgtg ggtcaatatt tgtaattcga ttttagggaa atatttaaag aaatattttc
    30241 gttttatttc tttaaatctt caagaatatt gatagttttt ttgaaagtag gtgtgtatta
    30301 tgaagaatga gaaaaaaatt acagtcgcat tgctcattgt ttatttattg attttatcat
    30361 ggattatttt atttaagcta caattttcat tttctgtgtt agaccacata agacaaataa
    30421 acttaattcc ttttggtggt tcagttattg caaatggaaa aattgacttc gatgaaatta
    30481 taaacaatgc cattgttttt atccctgtag gtgcatattt cagtttgctt tttaaaaata
    30541 aatctgttct aaaagcaata ggttctgttt taggaattag ctttgtatat gagataattc
    30601 aatttatatt tgctatcgga gcaagtgata ttacagactt aattagcaac acattagggg
    30661 gtataattgg aattgcatta gtatatgtat tatctattgt tctaaaggat aaaacacata
    30721 agatattaaa tcgaatagca atagtatgta ctgtattagt agttgccttc ttatttatgc
    30781 ttcttgctgt aaataatata ttttaatgcc atgttaatta catgttgttt tggaaaagac
    30841 tttaatctgt caaagtttgc ttttgaaatt gaacatgaaa ttacaccaat ggaacaaatg
    30901 aaagctataa atttttcatt ggagaaagat gactgtacaa gtaaagtatt aatatacaat
    30961 ccagacaaat ggaaatatgt agaatactat ttctataaag atagacctat taaaaaacta
    31021 ttggaaaaat ttattcaatt ttgcatttgt cacaataaaa tatgttttga aatattgaat
    31081 acttaaatat gcaaaaaaaa caaatgtaac tggctatggg gctatatcta ctaaaaagct
    31141 atgtgatagg ttgggattaa atttaaacat cacttctaga aggcatgaat acacaaggct
    31201 gacaaactat acagttatga ttaaacgata ccaacgggat tgaaaatttc gtgaagaatt
    31261 ttgagaagtc ctattacaca tgagcaacag caacgaagct attgaagcaa ggacgcaggc
    31321 aatctagcgg ttatctgaca aaggagttgt aggacttgaa tgtagatact atcagaacgg
    31381 atataccgac cagctctgcg gttacagatg tgattgtgtg gaatatcgag cagtcgggaa
    31441 cggacacttt atctgctacc tacgaagtag atcagcagat aaaagagaga gaacagacaa
    31501 cagcggtcaa ggcaacctat accgtaaaag tccatgtgga cgctgacggg gatatggtaa
    31561 tcgttcagaa ccttactctt gcaccagcaa tcgaaaaatc agactatgag ccaaagacat
    31621 cagaaacaga cgcaagcgtg gacgctgata ccgttaatga cgctaccgca tttctggaaa
    31681 cattctttga ggtgtacccg acagccacag aaaaagaact tgcctattat gtatcggaaa
    31741 atgtgattga acctatcggc agggactacc tttattctga attggtaaac ccagtcttta
    31801 caaaggacgg ggacaatgtg aaagtcaagg tagctgtgaa attccttgat aatcagacaa
    31861 aggcaacgca ggtgtcgcag tatgatcttg tactccataa ggatagcaac tggaagattt
    31921 taggataaca tggaaatggg cttgtatcat aattatagtg tgcaagccct tttcatattg
    31981 aagtatcaat aatcaataat tataattagg tacatgaatg gagggataaa atgttcaaaa
    32041 ttggtgagtt ttcaaaattg acacaagttt ctactcgtat gttgcgttat tatgatgaaa
    32101 cgggtttatt aaaacctgct aaaattgatt cattaactgg atatcgtatg tattcagcag
    32161 aacaaatctc aactttaaat agaattatat atttacgtga tagtggcttc aatgtcgctg
    32221 aaattgcatt agccctcaat ttcagtaata atgccattat agaacagctt gatagaaagt
    32281 ctattgaaat tatggataat atacaaaaag aacaagaaaa actaaagaaa attgagattg
    32341 ctaaaaaaga agtgttacat ggcaaaagtg aattgtattt taacataacc attaaagaga
    32401 taccgagtta ttgcgtttta tctttaagaa aagaaatacc aaattattat tatgagggcg
    32461 aactctggaa agaactctct gtttttgcta aagaaaataa aataaatatt tctgatgaaa
    32521 cattctcaat atatcacaat gaagaatata aagaaaaaga tgttgatgta gaattatgtg
    32581 ccattgtaaa taaaatagga aaagctacag gtgattttaa gtttcgtttt accgaaccag
    32641 tttctatgat ggcttgtact atggtatatg gagatttttt taatatttct tatgcttatc
    32701 gttcatttgc taaatggctt caagaaaaca gtcaatataa gatgaaaggt tcaagccgtc
    32761 aaatagttca tcgtggaccg tggaacgaga aaaatccaga gaactatcta acagaaatcc
    32821 aaattccttt agaaaatctc ttgtaaaaaa tgataatctg ccttgacttt aacatggtgt
    32881 taaggtttat cgtttattta tcaagaagaa aaaggaggaa aatataatga taacttataa
    32941 agtgaattca ataggtacta tctataataa tgaaaaaggt gctttcattc aacttgaagc
    33001 tgcatatatc ccagcattaa gggcattaga cggattcagt catataaatg ttttatggtg
    33061 gttcagtgat tttgataata cggattttag aagtgtattg gaaacagaac agccttataa
    33121 aaatgctcca gagaaaatgg ggatttttgc aacaaggtcg ccagtacgtc caaatccgat
    33181 tgctttatca gtagtggaaa ttatcaatat cgattatatg aatggaatga tacaaattgc
    33241 ttacattgat gctaatgaca aaacgcctat cttggatatt aaaccataca caccgagttt
    33301 ggatagggtg gaaaatccaa atgttccaga ttggtgtagt cattggcctc atagtttaga
    33361 agaatccgct tcttttgcat gggaaaacga atttaatttt taaggagtga tgaaccatga
    33421 gtttgcagga ttacattatt gaaaaaccag taattgatac agaacacctt attttaagaa
    33481 caatggataa aaatgacata gatgatttga aagaatggat tagtgatatt tctttatacg
    33541 agtattgggg gaaacgtcct tcaaaaagcg aaaaaaatcc agaagtactc tttagtaagc
    33601 cagaaaaagc tactaaaagt tttcattggg gaattgtcca taaacaagat agcaaagtga
    33661 ttggtgaaat atggatatac cttattgaaa ataatagaat ggcaaaagtg gctttcagac
    33721 tttctaagca atatcaaggt aatggactaa tgagtgaagc gttgaaaagc gtaattgaat
    33781 tttgtttttc aaaaacagaa ttacaaaaaa tatggacaga tgtgcatatt ttaaatcatg
    33841 cttcttacaa gacattggag aaagtcggtt ttcaaaggga aggacatatt cgagggggaa
    33901 aaatggtaaa tacctattgc gactactacc tatatggaat tttaaaatct gattatatca
    33961 ctagctgatg ttatggctaa tattgaagcg acgattggcg aagcaatgaa caatacagtt
    34021 ccaaaggtct atgatacgaa aagcattaaa agatatgtga tgaatttatt agatatggct
    34081 cattaaaaag taccaaagaa ataaaagtta ccatatagac aaaattggtt ttttatatct
    34141 tttgatttct tataagataa tggcattgtg cgacaagcta tacaaaaaat tttcatgtat
    34201 actttgatta taaattatac aaggagctaa ctatatataa atcaagtgat ttttgataat
    34261 tagctgaaat attttttaga tcattgaaaa gtgaattttg tgtacactga aaactactgc
    34321 tttatgcagt actttaaaaa tattcaattg aatgctttta aataagcagt ttttcatcaa
    34381 attgtatgtt ttcagacaac agtttataga ttactcttat gagttttcgt acggaatgcc
    34441 cctgagcaca tctatgggat ttaccttgta agcgttttag tttataatac ttattgaaag
    34501 tgagattgta tttgcatact gtaagaatgc attgatatag actttttctt aggtagcgcg
    34561 aaccacgttt agacaatgct gttcgaggtg cactgtattc accggactga taagtaccgg
    34621 ggtctacacc tgcaaatccc atgactttga cagcagagct gaagttatga atatcaccaa
    34681 tctcgcttaa aatcgacatt cccgtataaa tcccaatacc gggaatcgag aagataggag
    34741 aatctagttt gacagctaat tcttctattt ttttgtctac agttttaagc tgactggcta
    34801 acagttcgat attttcaata agcattttga tttcaagggt aacagcttcg ttgttttcgc
    34861 cgatagattt ctttgcactg gaacgaagag atgatgcatc tatattcaca cggcttccac
    34921 tgcttttagg cttgagaact tttttaagat gagcaagatt ggcactggct aaattataag
    34981 aacttccaaa ttcttttaag acagccatat aagctttgga atatttagtt ttaaaccaag
    35041 tattgaactc aggaataacg atatcaatac atttctgaag ttggtttttc ttctggttga
    35101 tatcttcagc aatagaagca tgataacggg ttaattcacg agcctgtttc atatcgagct
    35161 tacgctgagt gactctagta taatctctat caaagagtac tgaacaaata agaaaagtat
    35221 cttttttatc attcttagtc tttcttattt tgcttttgcg cttggcatca gttgttaatg
    35281 gatttatcaa agcaacttca taatcatgag tgattaaaaa attaataaga ttatcaccgt
    35341 aatgaccggt agcttctaaa ccgacaatat gtctaggtga tttaaatttt ttaacagctt
    35401 tgataaaaga atcgaatccg tctagattat tagtgaagaa aaaaggttca ataaggactt
    35461 caccttcaga agaaataacg caggcacagt gattgaattt accaacgtct ataccgatgt
    35521 aaaacataaa aagcacatcc tttctttata taagatagtg atatgtgatt aaaccacaaa
    35581 cagagtatca aagagcctgg tgcaaaatac aaattcgaag acttatctaa ctgatcacaa
    35641 ttaagataca aagatgtggc aataacctcc ttcaagtagt caagctacag gtgctgaaag
    35701 aaaaagtaca cattcacaat tcaattctaa acaaaattta atgaaaggaa aaggtgtata
    35761 taattgacag atagataagt caattacaat ataccaggta ctgatatgta cgcatatgaa
    35821 aatattgaaa tgtcattaaa ctatattgaa caacatttaa gtgagaaaat cgaaacagag
    35881 aaactggcgg aaattgcctg tctttctaca ttttattacc aacgcttgtt taaaaagtta
    35941 gttaagaaat cagttcaaga gtatatcaaa cttcgccgtt tagcaatggt aatagcagaa
    36001 ttgaataatt cagaggaaag aatattagac atagctttaa aatatggatt ttcagaccat
    36061 gctaacttca caagagcatt taaagaagtt taccatatta ctcccgatga atacagaaag
    36121 aatgttccta tgttaaatac atttgacaag ccagaacttt cttcacgtta tatcatgatt
    36181 gatgaaaatg tcccattgat tgtaggaaat attgttttgg aaattcaaag aaaaacatta
    36241 agaacaaagg aaacatattt tggttttgaa aaaacagtaa atattgctga acaaattcct
    36301 gctggagaaa gtacaggaat tgatgttcca ggtcagctat ggcgcgaatt ccatgcaaag
    36361 aaactgcaaa ccgaaaatgc catttttaac aatgatattg aacttggtgt atctcactta
    36421 gcaagtccag ataaaggaac attcctttac tttgcaggag gtatggtttc atcaacagat
    36481 gtatgtccta aaaatatggt acaacataca ttaccagcag gtgagtatat tgtatgcaaa
    36541 attgaggcag aatcttttga agaacttgtt actactgctt taaatcaagc aaataagtat
    36601 ctgtttgaaa cttggcttcc aaatcataat ttggttacaa agccattcat ggcagaaaaa
    36661 tattacaaag gaaatgcaga gattaattat atggaagtat ggataattcc tgtgtaaaca
    36721 gtaaatgcta aataggaatt atctgacgta ataaaaagga aatagaaagc ttataaagga
    36781 gggcatagtc cgttgtgata aacataaaag atataaatta tgtacctaat attagtgaaa
    36841 taagcgttta tattgaaaac tctgcatttg atgagttgta tcagcatatg aacaatgaat
    36901 ataaagcagt ttgcaaaatc gaatacagta aagatgtctg ggcacgaggt tggaacgtaa
    36961 agtttaaaaa atctgggaaa tcattgtgtg ttgtctatcc gaagaagaac tactttactg
    37021 tattggtggt tgtaggtaat aaagagaaag aagatgttga aaacttgtta cctaggcttt
    37081 ctgaatatgt acaggaaatt tatcgtaaca ctagagaggg aaatggacaa aggtggctta
    37141 tgatagactt aaaatctaaa aataatgtat atcaagatgt attaaggctt attgaaattc
    37201 gttatgccat aaaatgtgta aaaccgtaat aaaaatattt ttcacatacg taatctcaag
    37261 gtattatcgt tgagacaagt aacctatata attatgggaa atgagatttt tagatactta
    37321 agaaagtact ttagggggct tgcgttatgg aaagatttaa gtacaattcc acacaatcaa
    37381 atacaatcta ataaagcttt actaaaaatt gaatgcatta cagctttaaa ggctcgtaca
    37441 aactatatgt tttgtgcggg cttttttcat ttaataggaa tttggattcg tcagagagta
    37501 tattataaaa ataaaacgat aagggtacct taaaaataag gtacctcagg ctgtagacaa
    37561 agtcccggac tctcgtctgg gcttttctta tatttgagtt atccacaatt caacttttta
    37621 agagttatct tattaagaaa aaagtgtatg ttttccaccc gatagtttta cttctgtgat
    37681 atttgcagat gccactcaag tagaaagtgg tggtacggca gaagatgtca tgcagagttc
    37741 agaaagtctg ggactaccgc ctaattcctt agacacggaa agctcaatca agcaggggtg
    37801 taagtatttt gcgtccctgc tttcttcctg caaaaatcaa ggtatcgacg atttgaatgt
    37861 agcgatacaa tcttacaact atggcggggg ttatgtgggc tatgtggcag gaaaagggaa
    37921 aaagcacact tttaatcttg cagagagctt cgcccgtgag aaatcgggtg gaaagaaagt
    37981 aacctacgcc aacccgatag ccgttgcaaa gaacgggggc tggcggtatg gctatggaaa
    38041 tatgttttat gtggaattag tcaatcagta tttaactgtt ccgcaggtgt cgggggaact
    38101 ggcacaaaag gtaatgaatg aagcgttgaa gtatcaaggg tggaaatatg tgtttggggg
    38161 aagcaatccc aacacttcct ttgattgtag cggacttgtg caatggtgct atggaaaagc
    38221 tggtatctac ttaccgagaa cggcacaaac acagtatgac gcaacccagc atatcccttt
    38281 atcgcaggca aaagctgggg agttggtctt tttccattca acctataacg ctggttcgta
    38341 tgtaacgcac gtcggtatct atgtgggaaa taatcagatg taccatgcag gcaatcagcg
    38401 gttatctaac aaagaaattg caggacttga atgttgatac tattagaacc gatatactga
    38461 ctagccccac agttacagat gtaattgtgt ggaatatcga acagtcggga acggacactt
    38521 tttctgctac ctacgaagta gatcagcaga taaaagaggg agaacagaca acaacagtca
    38581 aggcaaccta tacggtaaaa gtccatgtag acgcttacag ggatatggta attattcaga
    38641 accctaccct tgcaccagca atcgaaaaat cggactatga gccaaagaca ccagaagcag
    38701 acgcaagtgt agacgctgat accgtgaatg acgctaccgc atttctggaa acattcttta
    38761 agctgtaccc gacagccaca gcaaaagagc ttgcctatta tgtatttgga aatgtgcttg
    38821 aacctatcgg cagggactac ctttattcag aattggtaaa cccagtactt acaaaggacg
    38881 gggataatat gaaagtcaag gtagctgtaa aattccttga taatcagaca aaggcgacgc
    38941 aggtattgca gtatgagctt attctacata aggatagtaa ctggaaaatt attatgtaag
    39001 acattttgtt ggcaggatct attttcggat atagtctgcc ttttctgtag caaatgtact
    39061 gcactgacaa gctatttcat tgtattttaa gcaattaaat ctttcttgac aattttatct
    39121 taaatcatat atttatggat attgactttc tgcttgcaat aagttataat atgactataa
    39181 aaccaaatta gtcacatagg aggtatgtgg tatgtataaa gaagttggta tttcttcaaa
    39241 taatgttccg ataacacttt ctatctggta tacagaaaga agtagtccta ccattgtttt
    39301 tttgccgggt actatgtcac accctctcat gtatgagaat tttctatgtg gtttgagtga
    39361 aagaggattt aatataatag gcattcacta tctttcacac ggaaaaagcc ctaaaatcaa
    39421 aaaaaattat acaatggaag atttgctgca taatgtatat gacgcaacaa cttacggaat
    39481 agaaaatttc ggtgaaaaca tagctgtcat gggttccagt caaggcggga ttttagctgc
    39541 aatggcggca ggtagggaca caaggttaaa agccgttttt ccacataata tcatgctgac
    39601 tacattaaaa gaaactatga gcttaacaaa atatcccaca tggtcgcacc gttttatggg
    39661 gctgatacag tggggctttc gtgtaggtgg aaaattgctg cctaactaca aggttccagg
    39721 agacgcttat ttagactatg acagagtatt ttatagtgaa caggcaaaac aagattgctt
    39781 aaatgaccct atgcttttac cctattatcc tttaagattt gtcgccagct tatttaatgc
    39841 tgacttatcg tgcctggaga atggggctat acaatgtcct gttattttaa ttactgccaa
    39901 aggcgacccg ctttttagct ttgattatac caacaaagta tttgatttaa ttcatgtacc
    39961 gcaaaaggaa ttacttgctt tagagcttaa tcgtcacatg atttttaatg aggacacgga
    40021 cactacaata aacgcattgg aagctacctt gcataaatat cttggatagc atattgatta
    40081 tataatcagt aaatgttata cttttcatga cgatataaat attttagtca taaggaggtt
    40141 atgagtttgc cacgatttac ggaacaagaa aaagaaatca taaacagtaa acttttgata
    40201 gagggagaaa agctgtttgc attacacggc ttaaaaaaag taacagttga tgatttagta
    40261 gctgctgtaa acatatcaaa aggttctttc tatgcttttt atcccagtaa agaacatcta
    40321 tatgttgaaa tcaattttcg cttacaaaaa gagttattta caagtataga aacaaccatt
    40381 aaaaagaaaa agtataagaa ccatagagat ttagcaaagg acgttatcat gctaagttta
    40441 acgggggtga ttacttcgcc tattctttca cagattgatt tatctttaat ggactatctg
    40501 caaagaaaaa tatcgtcaga tatttttgaa aatcatatgt acagtgatat tcgtatattg
    40561 gagatattgg aagatttagg ggttgtattt ttggttccgc atacggttat tataaaatcc
    40621 ctgtattctg ttttatcctg tttggaacaa ttcaaagagg acgaagaact gaatatgata
    40681 caaaatcttt tagtgaatgg tattattcag caagtagtag cagagtaaca attttgtttg
    40741 gttatttgac aggcaggtag ccaaacaaat tttttacagt ttatatgact atatatctat
    40801 aagcgtcata aagttaaagg aggaatgaat aatgattgat gtaaaaaaac tgtattttag
    40861 ctatactgac aaaccgtttg tggagaatgt aagtttccat gttgggagag gagaaatttt
    40921 tggtttttta ggaccctctg gtgctggaaa atcaactatt caaaaagttt taactgggct
    40981 taatacaaga tataaaggca gcgtaaaagt tgcaggtaca gaaataaggg agcatacaaa
    41041 tcagttttat gaaaatattg gggtagactt tgaattttca acctgctacg agaaattcac
    41101 agcacgacaa aatctcgcct attttgcttc tctatatgaa aagcaacctc ggtctattga
    41161 tgaattattg catatggtag gcttggaaaa tgacggggac aaaaaagttg ctgacttttc
    41221 caaaggtatg cgttctcgct tgaatttcat taaagcgtta gtccatgacc ctgatatatt
    41281 attccttgat gaacctacaa gcggtcttga ccctacaaat agtcgtttaa tgaaagatat
    41341 tattcttgca gaaaagaaac gcggaaaaac gattatcatt actactcaca atatgtttga
    41401 cgcaacagag ctatgtgacc aagtagcctt tatcgttgcc ggaaaagtca gtgcattgga
    41461 tagcccgcat aatttgatta tgtcaagggg agctgccaaa atacaatata cctactatga
    41521 taaaggcgag aaaaccggag aatgtctgct tgatagaacc acagaagata agttgttaaa
    41581 aagtttaatt tccgaaaacc gtttgctttc tattcatagt agtgagccta cattaaatga
    41641 tatttttgtt gatattacag ggaggacact tcaatgagat tgggacgttt aatttgtggt
    41701 gatatacatt tccaatggaa atatggtttc tattttatct actttatatt aacagtttta
    41761 tatgtgtgcg ggattgccgc tttgccggaa cattggaaaa cggatattgc ttctattatg
    41821 atctactctg acccggcagc aatgggatta ttttttatgg gagcgattgt cctcttagaa
    41881 aaaagtcaaa aggtgttaaa tgctatggta gtatcgcctg taaaagtatc ggagtacata
    41941 ctttcaaaaa caatcgcgtt gattgctatt tctacggtaa tcgcattgat attaggggta
    42001 gtttccgaca gcaatcattt acttggtatc acagttggta cggctttgac ctcagcaatc
    42061 tttactatgc ttggaattat tgcagctaca aaaatttcaa acttgaacca atttctcatt
    42121 gtgattatgc cgatagaaat tgtttgcttt gtaccaccta ttgttggtct gtttgtgaag
    42181 ttaccatatc tgttccgctt ttttccattc acagcttgca tgaaccttat aactggaaaa
    42241 agcgttttac tatcgtttga tatggtactt gtaattgcta cgttgattat cctctacata
    42301 gttgcgcgac atacagttaa gcatatgtgg aaaagtttag gaggtgtaaa gctatgaaaa
    42361 ggatattatc cagtaccata caggtattta gacaaatcaa gagtgaccct atgatgtttg
    42421 cagcttgttt tacgcctttt gtcatgggag ctttgattaa attaggtatt ccctttcttg
    42481 aaagaataac ggatttttct ttgcaagcat actatccgat ttttgattta ctactttcta
    42541 tcatggctcc tgtattactc tgctttgcat ttgcaatgat tacattagag gaaatcgacg
    42601 ataaagtgtc acggtacttt tcgattaccc ctcttggtaa gtcggggtat ctctttacaa
    42661 ggttgggagt accctcaatt atttcggcgg tcatcgcttt tattgtactg ttactttttt
    42721 cattagaaaa gttgtccatt ggaatgacaa tatgtttggc gcttctcggc tcagtacagg
    42781 caattattgt ttcacttatg attatcacat tatcaagcaa taaattagag ggtatggcag
    42841 ttacaaaact agctgcactt acattgctcg gaataccagc cccctttttt attgatagtt
    42901 actaccagtt cgcggttggc ttccttccat cattttgggt agcgaaagcc atgcagaatg
    42961 aggcggttct ttatttctct ataggattgg tggtagcttt agtctggtac tacttcctta
    43021 caaaacgtct gtttcggaag ctggcaggat aaggaggcat attatggaaa agggtagact
    43081 tctgcaaaac ggaaaagtaa atagagctat tcaggtttcc aaaatcacac tatttatcaa
    43141 cctaagttta tcgttactta aatttactgc cggatatata ggaaagtcaa gtgccatgct
    43201 gtcggacgct gtacactcgg cttctgatgt attaagcaca atcgttgtga tggttggaat
    43261 taaaatatca gaaaaacagc ctgatagaga acatccttat gggcatgaac gtatggaatg
    43321 tgctgcttcc attattctct ctgttgcatt ggccataaca ggtgctggaa ttggatattc
    43381 aggtatcaag aaaatctttt cagaacagta caatacgctt tctactccta gtggaatcgc
    43441 acttacggcg gcagtcttat ccattgttat aaaagagtgg atgtactggt ttacaagaaa
    43501 tgctgcaaag catacaaatt ctgacgcact tatggctgac gcatggcacc accgctctga
    43561 tgcactctcg tcggttggtt ccttaattgg cattttagga gcgcgtttag gctatgctat
    43621 tctcgaccct attgcaagcg ttgtgatttg cggttgtatc ttaaaagctg ctttggacat
    43681 tttcaaagaa agcattaaca aaatggtaga ccatagttgt gacaatgcta ctgaaacaaa
    43741 gattagggaa gttgtgcttc agcagcaagg ggttgatggt attgatgaat taaaaacccg
    43801 catgtttgga gcgaaaatgt atgtagatat tgaaatatta gctgatggta atttagctct
    43861 ttatgacgct catagaattg ctgagggagt ccatcaaacg attgaaaaca actttccaca
    43921 atgtaaacat tgtatggtac atgttaatac gaatgaactt ctctattctg cataggacag
    43981 ggaagatgat ttaaggctat tgaaagggaa aggagggaag taggttatgg aacaatactg
    44041 gtggattatc attgttgtgg ttttgattgt cgttggttta cttaaaattg taattatgaa
    44101 gaagtataac caaaggagaa attctaacaa tagtccaaaa gatagcgagg acgacaagat
    44161 gtattaaggt ttattgaaat ctgttatgtt gtagaatgat gtttttagat actagaaagt
    44221 gctttaaggg ggctaacgtt atggaaagct ttgagtacaa ttctttccaa tcaacagtat
    44281 tttgcataga gaaatgtatg taggaaatat ggtgcaaggc ataaagcacc agcgatcgag
    44341 agatcaacct atgaaccaaa ggcacactac attttatact acttgacaag taaacgagcg
    44401 tttactataa tggtttacag aagtaaacgt tcgtttacta aagaaggagg ttgtaaatgt
    44461 ctgacacaaa agaaaatata ttaaaaattg cactacactt atttgcagtg aatggttatg
    44521 aggctgtttc tgttagtatg attgcggaag ccttgggaat gacaaaagga gcattatata
    44581 aacattataa aaataagcaa gatatttttg actgtatcgt agaacgtatg tatcaaattg
    44641 ataataagcg agctaaggat tatcaagtac cagaaagcac aatagaggta acccctaatg
    44701 catacagaaa tacaactatt ggaagtctaa taacatttac tcttgcacaa tacgattttt
    44761 gggtgactga tgaatttgcg tctaattttc gcaaaatgtt aatactagag caatatagaa
    44821 atttaaaagt gaatatgctt tatcaaaaat gtcttgtaaa tggtcctatc agttatatag
    44881 aagatatatt taatgaaatg attgaaaaag gtgttcttca agagtgtgat acaaaacagc
    44941 ttgctattga atattatgcg ccattatttc tattgataag tgtagcagac gcagttgaaa
    45001 attgttggga tttaaaaact caattaacta cacacgttaa acaatttgtc aagaaatata
    45061 ttataaatga gaaagtaaaa taatccttta ttctgtccta aatataaacc taacttttta
    45121 atttcagcag agaaactcaa aatgaagatt gttataagtc acaaatatta actattaaaa
    45181 tattacaata acggagatga gaaaatggaa tatattcgca gtaaaaaata ttttacaccc
    45241 gacttacaga caaaaattat ggggccgaat cctataaaat tacaagagga actattacaa
    45301 aatcataaaa tcccgtctgg tagctatgtc tgtgaccttg gaagtggaca aggtttaact
    45361 tctgtatttc ttgcaaaaga atatggattt gttgtttttg cagttgactt atggagtgag
    45421 ccaaaagaaa atgaagaatt tttcaaaaaa atgggattat cttctgcaca aattaaatct
    45481 gtaaaagctg acgccaatga acttaaattt gagaaagagt tttttgatgc tgtagtaagt
    45541 acagattcat ataattattt tggacgtgac cctatatatt tagatgaaaa attgcttcca
    45601 tttgttaaga aaggtgggtt tgtttatatc acagttcctg gcatgaagaa agattgccat
    45661 gagaaacttc cagaagagtt attgttatca tggacaccag aagaacttga ttatatacat
    45721 gatataacat attggtctaa cataattagt cattgtaaaa aggcagattt aatttcaata
    45781 tatgaaatgg aaagtaattc ggaagtttgg tcggattgga taaagcaaaa taatgaatat
    45841 gcaataaatg accgtaaggc aatagaagct ggcggtggag attatctgaa ttttattgct
    45901 ataattctac aaaaaaaata aattctttag aaccaaacac tataatttgc gttctaatat
    45961 gtttaccgat tgacatcaat aattttattt ttgagggtaa tgatttgaaa atagaagaat
    46021 aattccagtt tgtaaaagtc aataaatcca aagataaatt tgtatttgaa aggagttttg
    46081 aaaaatgaaa aaatatgagt atgtagaaat taactataat gcgaaaggag ttgtattcct
    46141 ttgtactgat aaacacaaag aaattattga cagttacgca aaaacggggt atcactacgc
    46201 agggtttgta cccacagaaa ttgacgcaaa aggctgtatg cgaaagatag atttaatttt
    46261 tgaaaaagaa gattaactta ttttagggag ttggttctaa tgataggact taaaagacga
    46321 aatatgaatt atattatgaa agttggtgct aaagctcaag gtgtttctgt atctgaattt
    46381 agggctatgc tacaagatac catagacaaa actatggaaa gtacagaccc agacgtgcag
    46441 gcaaatttta aacgatactt tggaaataag cgcccaacac cagaggaata tatttatacg
    46501 attaccaaaa agacaaaggt taaggtttaa atgttgtaaa gggggcttgc cgtatgaaac
    46561 gctttatgta caattcagta tattcaagcc caatttaaca gcatactatt tacaattaaa
    46621 tacgctatac cagtagctcg tacaaaattt atattttgtg cgggcttttt taatacccga
    46681 aaagtttttt taattatttt catttttttg gaagcaaatc acaccttgct gtacagatat
    46741 ggtgcggaaa gagggataaa cacttttcaa ttcatagctg gaaaggaggt gagattatga
    46801 aaccgtctga cttccaaaaa acaattcagt gtcagtttga atgtaagctg aaaaaagttg
    46861 tcaaaggtat tatctggaac taccgtaaag aattaaaacg aagaaaaaac aaggaaatat
    46921 cttttagcga attaccagac attacggtag aaagtctggc aacatgggac gactacgaaa
    46981 caaactacac aatttttaat gtgtgtggca tagatattaa agtccttgat gatgaacttg
    47041 cagaagctct caaacagcta cccgaaaaaa aacgtaacaa tgtattgatg tattacttct
    47101 tggaaatgag tgatacagag attgcagata ttatgaatta taccagaggg gctgtgttca
    47161 aacacagaca ccgtgcccta gaaactatga aagagatact aaaggagaag aaatagaaat
    47221 gaagaaaaag tacaagcacc cgtcttatta tttgatttca tcagctatgg acggggatga
    47281 aactgcgatt gagaaattac tggtattcta tgacccgtac atatcaaagt gttgtttacg
    47341 tcctttatat gatgaatacg gcaatgttta tattgttgtt gatatggaat taaagggacg
    47401 tattcgagaa gccttaatca aaatgatttt agactttgag attgatatag atatagaaac
    47461 cgaagaagaa taagcagata ccagcagggc gtgattggtt aaacctcctc tttcctgccc
    47521 tgctcctgct tttatacatg gaagcctacg gctttcgccg attgttcttt gacaactgaa
    47581 taaagcagac agatacgttt gtgatacagc gagccacggg gactgtacgc catgaccttt
    47641 ccgacaagaa agtgagcgac ccacgcagtg atccgagagc aacttgtgga atggtcgctt
    47701 tgccatgacc tgctatcaca gaataatgat acgtccgcat ggtgcggttc tgcccacaag
    47761 aatgggaata gttgagatac taacggagct ttccgaagcc gtctgtctgc ttatgataag
    47821 acatacagaa aggagtgttg caacatgaac aatactgatg tgcctatctg ggaaaaatat
    47881 acccttagta tagaagaagc gtcccgatat tttcgtatcg gggaaaagaa gttgcgtagg
    47941 cttgccgagg aaaatctgga ctctggctgg gttatcgtca atggaaaccg tattcagata
    48001 aaacgaaaac agtttgaaaa aattattgat acactggacg aaatctgatg tcttagagcc
    48061 gtagatatgg tataatatta gtagcgtatc acggctcatt ccatgacgga aaggagcttt
    48121 acaccatgtc aaaagtgaaa agacaagaca gtaaaggacg caatttgcgt cttggagaga
    48181 gccagagaaa agacggaaga tacgtttata aatataccga tatattcggg aagccacagt
    48241 ttgtatatgc ttggaaactt gtacccacag acaaaacacc tgctggaaaa cgtgatgata
    48301 tatcgttgag ggaaaaagaa aagcagataa aaaaagacct tgacgacggt atcgacacag
    48361 tgggtggtaa aatgacagtc tgccagcttt acgaaaagaa gaacaatcaa agaaagaaca
    48421 tcaagagaaa cacaaaaata ggacgacagt atcttatgaa cgcactggaa aatgacccat
    48481 tgggtatgag ggcgattgat acggtcaagc agtccgacgc taaagaatgg gctatcagaa
    48541 tgaatgacaa aggttattcc tacaaaacga tagataacta taagcgttcc ttaaaggctt
    48601 cattctatat ggcgatacaa gacgactgta tcagaaaaaa tcctttcaat ttcaagttga
    48661 gtgatgttct ggaagatgat acggaagcaa aggttatcct aacaccagag caggaagaaa
    48721 aactccttgc ctttatggaa acagataatg tctacagcaa gtatcgtgat gaagttattc
    48781 ttctgctgga aacgggactt cgtatctctg aattatgcgg actgacaaca cacattgata
    48841 tgcttaaccg tgtgattaac ataaaccacc agctattgcg tgatacagag gtgggttact
    48901 atatttccac acctaagaca aaaaacggga agcgtaagct tccattgaca gaaagagcat
    48961 atcaggcact tgaacgaatt ttaaagaacc gaggaaaagc acaaccgctt gttgttgacg
    49021 gttacagcaa cttcttgttc ttgaaccgtg agggattgcc aaaggtggca ggaaattatg
    49081 aaagtatggt gcgtgggctg attaagaagt acaacaagac gcataaggac aagttgccaa
    49141 acatcacgcc ccattcattc agacatactt actgtacgaa catggcaaat aagggaatga
    49201 accctaacac cctgcaatac atcatggggc acgcaaacat aaccatgaca ttagggtatt
    49261 atgctcacgg tactttccag tctgcaaagg ctgaattaga aaggctggct tcttaatatc
    49321 gaaaatgtat cttactactt atttactact tttggttgca ttttcatgct ggtaaatgcc
    49381 atcttatgcg aggtatcttc caaaaggaaa atgccaataa agcccgaaaa tatgggatat
    49441 atcggcattt accaacttat gaaaagataa tcagaaattt agtaagtttt tatatcaaaa
    49501 atagtaataa agtctatata catttagaaa atactgttta aaaggagtag gatttatgat
    49561 aaaaaaagag atatctaact ttagaggaag tagtgattat gtagtatcac cagaattgat
    49621 ggcatcagtt aatgttgcta ttgctcttga aaagccactt ttgattaagg gcgaacctgg
    49681 aacaggtaaa actatgctag cacaagcaat ttcaaatgaa ttaaaaaaag atttagtaat
    49741 ttggaatata aaatcaacta caaaagcaca agaagggctt tatgtatatg atactgttca
    49801 aagactttat gatagccaat ttggaggaga aggtgtagat gatattagca agtacataaa
    49861 atatggtaaa ctaggagagg cctttagctc aaatcaacaa gtaattcttt taatagatga
    49921 aatagataag gcggatttag aatttccaaa tgacttgctt tgggaattgg ataaaatgga
    49981 gttttacata aatgagacaa aagaaactgt aagagctaaa caaagaccaa tagttattat
    50041 aacttcaaat gcggaaaaag aacttccaga tgctttttta agaagatgca tatttcatta
    50101 tatagaattt cctgatagag acatgatgga agaaatagtt aaagtacatt ttgataaagt
    50161 agaagaacac ctattggaac aagtaatgac tactttttat tggattagaa gtttaaagga
    50221 catacaaaaa aagcctagta catcagagtt aatagactgg atacaagcat taactttgag
    50281 tggaatgcct attgaaaaaa tagaaaaaga agttcctttt gcaggaatac tacttaagaa
    50341 taatgaggat attgaatcta tgcaaagaca cttatagtta ataggagctg gataattatg
    50401 tttattgaat ttttttattt attgaggtca agaggattaa atgtatcttt aaatgaatgg
    50461 atgactttaa ttgaagcatt ggataaagga ctgtgctatt caaacttttc aaatttttac
    50521 tatctttgca ggatgatttt aataaaaagt gaaagtgatt ttgataagtt tgatgcagtg
    50581 tttttggagt attttaaagg tatagaacac caagaagaaa ttccagaaga gattatgaat
    50641 tggcttaaaa aaccagatat agatttagaa gagtttgaaa gattagaaca aaatccaaat
    50701 ataaatcttg ataaattaag agctaaattg gaggaacgaa taaaagaaca agactctcct
    50761 cataatggtg gtaattattg gataggcaca ggaggcactt cagagttagg acattcagga
    50821 aaagggcaaa ctggtatacg tataggtgga aattctactt atggtagagc tgttcttgaa
    50881 gttgcaggag aaagaaaata cagagatttt agagatgatg aagttttaaa tatgcgtcaa
    50941 tttcaagttg cactaaagag acttcgtcaa ttttctacca gaatagatgc tccaaagaca
    51001 gagcttgatt tggataagac tattgaagag acttgtaata atgcaggata tttaaagtta
    51061 ttttttgaga agccaagaaa aaatacagtg aagcttttat tgcttatgga ttcaggtggt
    51121 tcaatgagag ggtatagcac tttatgtaat acattatttc agtctgtaag taaatcaaat
    51181 cattttaaag atgtaaagat atattatttc cataactgta tttatgatag attatttaca
    51241 acacctgaat gttggttaag taaatctatt aatactgaat ggatattaaa aaatatagat
    51301 aaaaactata aagttataat tgttggagat gcttctatgt cgccttctga gttgcttcat
    51361 attggtggta actatcgtgg tccatataat tacacacctg gtatagagtg gcttaaaagg
    51421 tttaagagaa agtacagtaa aattgtatgg atgaatccag aacttagaga tggatgggat
    51481 tcaattaatt attggtatca aactcaaaga atgatacaaa gtgaatttaa tatgtttcca
    51541 ttgactgtaa aaggtcttga gaaagcattg aaaaatttaa tggtttcaag ataaagtaaa
    51601 tataaatgat ggtatgattt attcaaaatt gtaccatcat tatttatttt ttttaatata
    51661 tctattataa attgtataac tgaaacttaa aaaattatat agtctaatac attttagact
    51721 cacacaaaac atatacatta ataataattt tagaagtcga ggtgattata ataaattatg
    51781 aattaattgt aaaatacaat ggagatatat tgaggttaga agaagaatta ggtgtatcag
    51841 tagagatact taattcatca tatgcaataa taacatcaag taatgaagaa gatgtaaata
    51901 tacttttaac ttatccagaa attgaattta tagaaaagcc ttttatatta cagactcagg
    51961 atgtgcaaag tttttcaagt acaggaataa caggatttaa aaatagaact ggtttaactg
    52021 gaaaaggaac tataattggt ataattgatt cgggaataga ttatactcta cctgtgttta
    52081 gagatagtga tggaaggtct aagatattat attattggga ccaatctatt caaggaaatc
    52141 caccagaagg ttttagagaa ggaacattat atactaatga agatataaat aatgcaatag
    52201 atggaagtat gtatatacct atttcaacaa ctagcttaca tgggacacat gttgcaggta
    52261 tttgtgcaac tattgcaagt gatgccagaa taatagtggt tagagttgga aatatacaga
    52321 ccgatatttt ttcgagaagt acagagttta tgagggcaat taaatttatt ttagataggg
    52381 cattagaatt aagaatgcct gttacattaa atataagtta tggaagtaat gaaggttcac
    52441 atagaggaac atctttattt gaacaatata tagatgatat gtgtttattt tggaaaaaca
    52501 atatagttgt agcagctgga aataatgcag ataaaggagg tcataaaagg attaggcttc
    52561 aaaataatat tacagaagaa gttgagttta tagttgggga aggagagcgt atattaaaca
    52621 taaatatatg gccagatttt gttgatgatt ttagtgtaca tttggtaaac ccatcaaata
    52681 atcaaacaca agcaatctct ctaacttcag gtgagataag gaatactttg ggtgaaacta
    52741 gaataactgg ttatttttac cctatagcac cgtattctct cacaagaaga gttactctac
    52801 aattaagttc aaatacacag ataactcctg ggttatggaa aattgtattt gaaccaatag
    52861 atatagtaac tggtaatgta aatatatatt tgccaacttc tgaaggtctg aatagaaata
    52921 ctagatttct gattcctact caagagctta ctgtaacagt tcctggaaca gcaagtagag
    52981 ttataacagt aggtagtttt aattcaagaa cagatatagt atctatattt tctggtgaag
    53041 gagatacaca attaggtgtt tttaaacctg atttattagc tccaggtgaa gatatagtat
    53101 cttttttacc aggaggtaca agtggagcat taactggaac tagcatggct acacctcatg
    53161 ttacaggggt ttgttcattg tttatggaat ggggaattgt aaatggaaat gatttatttt
    53221 tgtattcaca gaaattaagg gcattacttc taaagggagc aaggagatta agtaatcaat
    53281 cttatccaaa taattcatca ggatttggtt ttttaaattt atcagatata gatttgtaca
    53341 ctctatctaa tataaatcaa gatttagaaa cagaggatat tggatataga agtattaata
    53401 agtcatttaa agatgaggaa aatagttata agtttataga tggttataat atgcaaatac
    53461 ataatgactt agagaatgaa atttatataa gtaaaaatgc aagtagacaa tcaggaatac
    53521 tatcaggaat agatatagtc catactcctg aatttgaaga agaactagct gggttaggta
    53581 tgagtcaaag gttttttaag ataagtgatt cattaggtgt actatctata aataatacag
    53641 actataatag catacaacga gttttgcaac ttccttcaat cataagaact gtatcgacta
    53701 caaagatgac attacttgga gaaattaatc gaggtacttt tggaggtgta gttgcaacag
    53761 aagaaatggg cgtaaatttc tttaaaaata atcctaatat taatataaca ggaagaggga
    53821 ctctaatatc aattgcagat acaggaatag actatctaca cccagatttt atttatccag
    53881 atggcacttc aaaaatagta tatttatggg accaaacaaa agaaggaact cctcctgatg
    53941 gtttttatat tgggacagaa tatacacgag aagatataaa tcgagctatc gcagaaaatg
    54001 accctagtct atctcaggat gaagttggtc aaggaactat gttgagtggg atatgtgcag
    54061 ggcttggaaa tgtaaatagt gaatatgcag gtatagcaga agattctgag ctaataataa
    54121 taaaactcgg caagatagat gatttttata atagtgcaat gttgtttgct gcatctcagt
    54181 atgcatataa aaaagctttt gaacttggaa gaccattagt tataaatatg tctttaggta
    54241 ccagtagcct agcaggttta accaatagga gtaatagtga aaaagcgttc tttactagag
    54301 ggttatgtat aacggcagga gcaggaaatg agggcaatac acagacacat acatctggaa
    54361 taataccata tgttggaggt tctgttgaag ttgaattaga gttaaatgaa gacgaggaag
    54421 aattatcatt agaactttgg ttaaatagac cagataaagc tgatgtaata atagtatctc
    54481 ccactggtga agaaagtaaa agtgttggaa tttcaaatta taataaagta acaggtttat
    54541 ttgatttaga ggggactgaa tattctataa catatatata cccaacaact ttttctggac
    54601 agcaatttac taatgttact ttaaagaatg caaaaagagg tgtatggaag attcgattag
    54661 taggagttta tattataaca ggaagatata acttatacct tccaaataga gaacttttaa
    54721 aaagtggaac tagatttaga gaggtagacc ctttttacac tataaattat cctgcgattc
    54781 aagatgattt aataaccata ggagcctaca atactattaa tggtagttta tggcaaagtt
    54841 catcaagagg gccaactatt gaagacaggt taaaaccaga tatagtagca ccaggtgtca
    54901 atataatagc tgcttatcct gggaacacgt atgcaactat aactggtaca gcagcagcaa
    54961 gtgcacatgc agctggtgct gctgcaatgt attttcaata tacttttgtt gacggtagat
    55021 atccaaacca agcatatgtg caaaaaataa aaacttttat gcaagctggt gctagaaaag
    55081 attcaaacac agtatatcca aatacaaatt ctggatatgg gctcttggat gttagaggaa
    55141 tgtttgatgt tttaagatag gagggattta tggaaaaatc ttattgtata atttatcaag
    55201 gtgatataga atctgcccta caagaaaatg gaataaatag gtatatggtt ttaaatagtc
    55261 agcttgcagt aatatatgta cctttagatt ttgatgaaac tattttaaat aatataatac
    55321 aagttgcttg gtgggaagaa tctgaaccaa tgagcagtct aattgaaata actaataatg
    55381 taaataatgg agaaaccatt acaacagcag ctgaaactga ttatatttat gaaaatccat
    55441 ataatgatat aacagggaga ggaattttat tagcagttat agattctggg atagattatt
    55501 tgcatcctga ttttataaat gatgatggaa ctagtaaagt tttatattta tgggaccaag
    55561 aagcaaatac aaatccacct ccagaaggtt ttatttttgg aagtgaattt acaagaagtg
    55621 agttaaatat agccataaat agaaatgatg ggagtttgag tcaagataat ataggtactg
    55681 gaactttagt ttctggtata ttagttggaa atggaaggat aaattctcag tacagaggta
    55741 ttactacaga atcagactta atagtagtta aattaaaatc atatacagat acttattatg
    55801 ctggaaggat aaattacagt gtttcagatt ttttagcagc tataacgtat gttactaaca
    55861 tagcaagaac agaaaataaa ccactgataa taaatttgac tattggagtt aagtcaagtg
    55921 ctgtagcaac tacttcaata ctagatactt ttaatatatt gagtagtgct ggtgtggtag
    55981 tagtgagtgg tgcaggaaat caagggaata cagatattca ttattctggc agatttagca
    56041 gtgtgggaga agttcaagat gtaataatac aagatggaga tgactatgct cttgatataa
    56101 cactaaatac gaatggtcca gataaagtgg gagcacagat aatatctcct tcaggtgaag
    56161 ttagtcatga tataaggtat tctccagact tttatatata tagaggaaaa tttaacttag
    56221 aaaatactac ttatgcaatg agatttattt atccatatat tacctctgga aaagaaaatt
    56281 tggagataag attaagagat ataaaacctg gagtatggat tttaagattg acttcagaac
    56341 ttataataaa tggagaatat gacatatact tgccaaataa aaatttgata gctccagaca
    56401 caaggttttt agaccctgat tcagtagcta ctataactat gtatgctgct agtgatgatg
    56461 taattacagt tggtacattt aacaataaga ctgatagtat gtggattggt tcttcgaaag
    56521 gaccaattag aggaagagga attaagccag atatagtagc ttcaggtgta gatataatat
    56581 ccacatataa aaatggaaca tataatacag gaacaggaac aggagtgagc agttctatag
    56641 tcacaggtgt tttagcctta ttgatggaat acttagaaaa acaggataat gtaccgagat
    56701 tatcattgtt tacacaagtt ttaaaaacat atttaatatt gggtgctaca aaacttgaga
    56761 tatatacata tccaaatgta tcgcagggat atgggatttt aaatttaaaa aatacgattc
    56821 aacagatagc aaatactcta tagataagaa cctatgtaaa attataattg attattagtt
    56881 taattgtatc aaatacttta gatagagtag aaaaatagca taaaaaataa atatgaaaat
    56941 taaaaaacag ttttatattt tgaatacaca aagtaaatat tttgtatata aataggtata
    57001 aattaagtat tgaaaagata tgatgttaat ttaaaaataa ttataattta gacttatgaa
    57061 ttttatttgt aaaattgcta aaactattac aaaataaata atagaggtga tagttttgga
    57121 aagtaaaaag gcaagagcat taaaaggtaa agataataaa agacttaaaa gttcactttt
    57181 agttggaaat gagtcgacag cagcatgggc tgatgttgaa aaattaaaac ctcatagtaa
    57241 agtttcaata ccttcaatta agggtgtcga agaagcaaaa gactgggttg acaatggaag
    57301 taaattataa aattttggaa attgaagtaa taaagaagta atgaagataa gtatttatta
    57361 tcattatgta aggtagaatt attatatttt aaataacaat cagtatcaat tatataaatt
    57421 tattgactaa aaatttataa attggtataa ttttgttaat tagcagaaat aataaaagat
    57481 aaatactgtg aagagaagag tagatataaa atgtagttct agcgagttga ggatggtgaa
    57541 agctcaatac gaggtttata ttgaagaaca tcttggagtt tctaatcgaa agcttattta
    57601 gtaagtagac ttagacggaa ggcaccgtta taggctttaa gcattgctta actaagaggt
    57661 cattatgaca actagggtgg taccgcgaga gtaatagtct ttcgtccctt tatataggga
    57721 tgaagggctt tttttattga aattatattt aaaagaaatt aataacaatt atactaggag
    57781 gatttaaaat gcaaaaggaa tttataacag taaaagaatt gtacagagaa aaagaacagt
    57841 acatagggaa gactgtaaaa gtagctggat ggataagaac atctagagta tctaaaaatt
    57901 ttggatttat agagctaaat gatggaagct tctttaaaaa tatgcagata gtatttgatg
    57961 agaaagtaga aaattttaaa gaggtaggaa aattagcaat aagctcatct atacttgtag
    58021 agggagagtt agtatctaca gaaggggcta aacaaccagt cgaaatacat gcaaaaagca
    58081 tagttgtaga aggggaatca gatagttcgt atccactaca aaagaagaga catacgcttg
    58141 aatatttaag aacgatagca catttaagac caagaagtaa tactttttca gctgtattta
    58201 gagtaagatc aatagctgcc tatgcaatac acaaattctt ccaagaaaga aactttgtat
    58261 atgcacattc tccaatcata acaggtagtg actgtgaagg tgcaggagag atgtttagaa
    58321 taacaactat ggatttaaat gatattccaa aaactgaaga tggcaaaata gactatactc
    58381 aagatttctt tggaaaagag gctaatttaa cagttagtgg tcaattaaat gccgagataa
    58441 tggcactttc ttttagaaac gtttatactt ttggacctac atttagagct gaaaactctt
    58501 atacaggaag acatgcttca gaattttgga tgatagagcc agaaatagtt tttgcagacc
    58561 ttgaagataa catggaactt gcagaagata tgataaaata tgttataagc tatgtattag
    58621 aaaatgctcc tgaagaaatg gaatttttta atagttttat agataaagga ttgttagaaa
    58681 gattaaataa gatagtaaac tctgagttta ttagaataac gtatactaaa gcagtggaat
    58741 tattaattga atctgggcat gaatttgagt atcctgttga gtggggatgt gaccttcaaa
    58801 cagagcatga aagatatata acggagcaaa tatataattg tccagtgttt gttacagatt
    58861 atccaaaaga tataaaagct ttctatatga gaatgaatga agatggtaaa actgttagag
    58921 caatggattt actagttcct ggagttggag aaatcgtagg aggttctcaa agagaagaaa
    58981 gagaagatgt attgttagat agaattaatg aaatgggatt aagagaagaa gattattggt
    59041 ggtatttaga acttagaaag tttggtactg ctactcactc tggatttggt cttggatttg
    59101 aaagaataat tatgtatatg acaggtatgt ctaacataag agacgttata cctttcccaa
    59161 gaactcctaa aaatgctgaa ttttaataat tatatataat aaaacttata taactttaag
    59221 tataaacact aaaatgactt gcatatttat ctattttaga gatatgcaag ttatttttat
    59281 tgtactataa atatataatt tgattacttt tattttgcga tataatactt ataggtttat
    59341 gtaatagtat gtaacctttt aataaaatgc aacacaatta cgtccttgtt cttttgcagt
    59401 atatagggcc ttatcacaat tacttataaa ctcatttaat gttattgtct ttccaatttt
    59461 ctttgtataa actccaatac taacagttac acattttaaa ggaacatcat cacgctcaaa
    59521 attgcaggac tctataattt ttcgtaaaca ttctgattta agtttagcat ctgcctcatt
    59581 acagttaaaa atacatataa aaaattcctc accaccatat cgtatagcat gttcattttc
    59641 agaacttgta aaatctaaaa ttactttggc aatattttgt agacaatagt ctcccttttg
    59701 atgtccataa ttatcattaa attgtttaaa ataatctata tctatcatca ttccagttat
    59761 aggttcttga tttgcatggc tgattaattt tttttgtaga atatcttcaa ggtatctacg
    59821 gttgttcatt ttagtaaggt catctgtcat aactttgtcg ttaagtaaat tatttatatg
    59881 agatatttct tcatattgtt gactaattaa aagtttatca tagtaagtaa atacacgatt
    59941 tctatatagc attgtagaaa caaaaatggc aagggatgtt ataaaaaatg tgttgacaat
    60001 attactaaac tcattgcctg gtatagtttg aatcccaggt aagaataaat taagaaacaa
    60061 aaagcatcct ccaaaaacta ttactgattc tccaaatttt aatactgaga aagctgcagc
    60121 tgctaacata atataactaa atacacttag gtcatttcca cctaactggt caagtagagt
    60181 aattacacaa ccccataggc agaaaaaagt cacataaatt atccataaat ttatataaaa
    60241 cgtacagcaa tccttttttt tattccaaag gtaagaaaat acaaacataa agacagaaga
    60301 tacacaaaat agtgttacat acatataaaa ataccattgg cgtcttggtg tgacaaaagg
    60361 gcctccttct ctagctgaga ttgatatcat cataattatt tgagatatta ctattattat
    60421 gcaaatgatt ttacccgtat gataaagata attgcagagt tctttctcaa cattgtcttt
    60481 gtgttttttg tctagaggaa atccaaaaaa tttttttatt ttttctaagt tcatataagc
    60541 ctccattaaa aataaagcat aaaaataagt attcctttct atcattagat atttggaata
    60601 cttcgtttat gatttaatta tatcaaaata tattgtgtgt aacaatctaa aattgtgata
    60661 gataaaattt atta
//
LOCUS       DAELLV010000004        54251 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.004, whole genome shotgun sequence.
ACCESSION   DAELLV010000004 DAELLV010000000
VERSION     DAELLV010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 54251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54251
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.004"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..757
                     /locus_tag="KPD58_000214"
     CDS             <1..757
                     /locus_tag="KPD58_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HBG0264005.1"
                     /translation="VAYIFYKIFMFIKDTRAEQVFKGIIFLLLATQLSNTFKLHTVYW
                     ISLKALDYGVIAALIIFQPEFRAGLEHIGRAKFNLFGKNVNTSEETLNRNIEEIVEAL
                     YSLSRQKIGALIIMERETRISDIINTGTIIDAEISRQLLINIFIPNTPLHDGAVVIRD
                     SKVKAAACFLPLTESKDLSKDLGTRHRAGIGVSEVSDCITLIVSEETGGVSIAKAGKL
                     YRDISRERMMNILRSNLKTNTETRSFFKGGIFK"
     gene            754..1935
                     /locus_tag="KPD58_000215"
     CDS             754..1935
                     /locus_tag="KPD58_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264006.1"
                     /translation="MINRLKNNTKIKIISFLSAIALWMYVMAVVDPEETKGFEDIPVS
                     ISNMSDLKDKNLVIYPDEKLTADVYIKGSLSKVQSVKKEDIHVYGTIEDPIQGQKEVY
                     LKANIPQGVTLEFKHDVLVVNLEKNIKEKRDIKVEVSGNSKRNINKIELSKEFVDVSG
                     PRTLVKEVKSVRANLDVGNETDDFSKKLQLIPLNSDGTKVEGVTLSSSFVTAKIELLK
                     EKIVPIRVSLNDNKDNDVLKNYKLSKEQVTIKGKKDDIDKISYIKTKPIDLADLANGE
                     EKEVSLEIPEGITLEDSSITIKLNDTKQLSAEFLYNAGDIELRNIPTNMDNPNVISDV
                     DIKVVVESTEDLSKLNKKDISLYIDLSQGPEEDSKYKIKYETTYQFKKINIEPNIVEV
                     E"
     gene            2239..3141
                     /gene="ptb"
                     /locus_tag="KPD58_000216"
     CDS             2239..3141
                     /gene="ptb"
                     /locus_tag="KPD58_000216"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HBG0264007.1"
                     /translation="MRSFEEVIKFAKERGPKTISVACCQDKEVLMAVEMARKEKIANA
                     ILVGDIEKTKEIAKSIDMDIENYELIDIKDLAEASLKSVELVSQGKADMVMKGLVDTS
                     IILKAVLNKEVGLRTGNVLSHVAVFDVEGYDRLFFVTDAAMNLAPDTNTKKQIIENAC
                     TVAHSLDISEPKVAAICAKEKVNPKMKDTVEAKELEEMYERGEIKGCMVGGPFAIDNA
                     VSLEAAKHKGINHPVAGRADILLAPDIEGGNILYKALVFFSKSKNAGVIVGAKAPIIL
                     TSRADSEETKLNSIALGVLMAAKA"
     gene            3163..4242
                     /gene="buk"
                     /locus_tag="KPD58_000217"
     CDS             3163..4242
                     /gene="buk"
                     /locus_tag="KPD58_000217"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBG0264008.1"
                     /translation="MSKIFKILTINPGSTSTKIAVFDNEDLVFEKTLRHSSEEIGKYE
                     KVSDQFEFRKQVIEEALKEGGVKTSELDAVVGRGGLLKPIKGGTYSVSAAMIEDLKVG
                     VLGEHASNLGGIIAKQIGEEVNVPSYIVDPVVVDELEDVARISGMPEISRASVVHALN
                     QKAIARRYAREINKKYEDINLIVAHMGGGVSVGAHKNGKIVDVANALDGEGPFSPERS
                     GGLPVGALVKMCFSGKYTQDEIKKKIKGNGGLVAYLNTNDAREVEERIEAGDEKAKLV
                     YEAMAYQISKEIGASAAVLKGDVKAILLTGGIAYSKMFTEMIADRVKFIADVKVYPGE
                     DEMIALAQGGLRVLTGEEEAQVYDN"
     gene            4232..4909
                     /locus_tag="KPD58_000218"
     CDS             4232..4909
                     /locus_tag="KPD58_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0264009.1"
                     /translation="MITNDKRIRIITGHYGSGKSEFAMNYVVKLRDMVSGKVAIADLD
                     VVNVYFRTREKKELMKSLGIQPIDSSINAPTLDLPAVSAEVMSPMVDHSYNSVIDLGG
                     DNVGARVIGRFSHLLKEGDYDMLFVINANREKTQTSEEVIQYIKEIEKSSKLKVTGLI
                     NNTHLIRFTTIDDVLRGQKVAKEVSEKCNIPIRYVACLENLVEQLPKDLEGEIFPIKL
                     YMREDWM"
     gene            4952..5167
                     /locus_tag="KPD58_000219"
     CDS             4952..5167
                     /locus_tag="KPD58_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG0264010.1"
                     /translation="MAKGKVSFNQERCKGCGLCVEACPVKIIQLDSNVINKKGYNPAT
                     VFEMEKCIGCASCATMCPDVVITVERD"
     gene            5196..6275
                     /locus_tag="KPD58_000220"
     CDS             5196..6275
                     /locus_tag="KPD58_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBG0264011.1"
                     /translation="MAKILMKGNEAFGKAAIEAGCKYFFGYPITPQSELPEYLSRELP
                     KIGGAFVQAESEVSAINMVYGGAGAGARVMTSSSSPGVALKQEGITYAVGAEVPCVVL
                     NVMRGGPGLGSIQPSQADYFMSTRGGGNGDYRTPVFAPATVQEAVDMIMEAFDVADYY
                     RSPVMVVADGMIGQMMEPVEFRAPEKKRELPPKDWATVGTKGKRKPNVINSLYLEPEV
                     LEDHCWHLQEKFDAMEKNEVQYEMYKTEDAEFVFAAYGTTSRVVKSAIDILREEGIKA
                     GLIRPKVLWPFPFEAFNQIPNARNILTVEMSMGQMVEDVKMAVEGKLPVYFHGRPGGM
                     TPTPAEIVEKAKKIIAGELVAGGAR"
     gene            6275..7027
                     /locus_tag="KPD58_000221"
     CDS             6275..7027
                     /locus_tag="KPD58_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBG0264012.1"
                     /translation="MAVVFKKTEGLQDTQTHYCPGCTHGIIHRLVGEVLEELGVLGDA
                     VGVVPVGCSVLGYKYFNCDTQEAAHGRAPAAATGIKRVHPENTVFTYQGDGDLASIGT
                     AEIVHAAARGEKITTIFVNNTTYGMTGGQMAPTTLVGQRATTAQSGRNAETQGYPIRV
                     SEMLATLTGAVFVERVAVDTPAHVRQAKKAIKKAFQVQQAGLGFGIVEVLSTCPTNWG
                     LAPNDALQWLRDNMIPYYPLGNFKNVEVEEVK"
     gene            7028..7585
                     /locus_tag="KPD58_000222"
     CDS             7028..7585
                     /locus_tag="KPD58_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBG0264013.1"
                     /translation="MSTARVICAGFGGQGVMSMGQLLTYAGMLEGKEVSWLPSYGPEM
                     RGGTANCAVTVSNEPVGSPLITDDATAAILLNIPAFEKFKDDVIPGGKIIVNSSLIKE
                     KVDRTDVDVYYIPANELAAELGNDKVANMIMLGAYLKVSDTVDIESVLEAFKKVFGPR
                     KEKFVPLNREALQKGMDATCSKATN"
     gene            7755..9101
                     /locus_tag="KPD58_000223"
     CDS             7755..9101
                     /locus_tag="KPD58_000223"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBG0264014.1"
                     /translation="MRKYFGTDGVRGVANTELTCDLAYKLGRAGGFVLAQGDHRVKVV
                     VGKDTRISGDMLEASLIAGLMSVGCDVITVGIIPTPAVAYLTRKYGADCGVVISASHN
                     PVEYNGIKFFNKNGYKLDDEIELKIEEYIDDIDKIDCLPIGENVGRKLHEHCAQRDYV
                     DYLKSIISTDFKGLKVVLDCANGASYKVAPIVFDELGASVISINSSPDGNNINYKCGS
                     THPEQLQRAVLEHNADLGLAYDGDADRLIAVNEKGQIVDGDHIMILSALNLKKNNKLA
                     QDTLVVTVMSNIGLTIAAKENGINLSTTAVGDRYVLEDMVKNGYNLGGEQSGHMIFLD
                     YNTTGDGVLSSLILANIILQEKKPLSEIASIMSQYPQVLVNATIKNENKNKYMEYPEI
                     KTEIERIESILDGNGRVLIRPSGTEPLVRVMLEGKEEGQIKELATNLANLIQEKLS"
     gene            9368..11200
                     /gene="glmS"
                     /locus_tag="KPD58_000224"
     CDS             9368..11200
                     /gene="glmS"
                     /locus_tag="KPD58_000224"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBG0264015.1"
                     /translation="MCGIVGYLGSRKAAEVIVEGLSKLEYRGYDSAGVAVNSSNEKEL
                     NIRKFKGRLSVLAEDLEKNPIDGNLGIGHTRWATHGEPSDVNSHPHFNQAKTIAVVHN
                     GIIENYMEIKEELISEGVKFESQTDTEVIAHLVDKYYEGNLLDAVYKTISKLRGAYAL
                     GVICKEHGNELVAVRKDSPLVVGVGEGENFIASDIPALLKYTRDVYFLENGEVVHLKD
                     ENVTVYDSNRNLVEKEVFHVTWDVEAASKGGYDYFMSKEIHEQPTGVRETLERRLDDN
                     GNIILDSINISKEDLEKINKVYIVACGTAYNAGLLGKYAIEKFVNIPVITDIASEFRY
                     SDPFVDENSLVILVSQSGETADTLAVLRDSKAKGARILSITNVVGSSIARESDDVFYT
                     WAGPEVAVASTKAYTTQITSLYMIALDFAIKKGTITREFYDSMISKMKEIPSKIQEIL
                     DNEEYIKEVAKTVVSSEHAFYLGRGIDYSLAMEGSLKLKEISYIHAEAFAAGELKHGT
                     IALIEKGTPVIAIATQEKLFEKMVSNMEEVRARGAYVVAIAQSHNKDVEKAADKIIYI
                     PNSDDILSPILAVVPMQLLAYHVSVLRGCDVDKPRNLAKSVTVE"
     gene            11652..12947
                     /locus_tag="KPD58_000225"
     CDS             11652..12947
                     /locus_tag="KPD58_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase"
                     /protein_id="HBG0264016.1"
                     /translation="MTNDFKKEKKEDYFVNLKAISTEVLQEKVQDNAWVIVDTRLNDA
                     YNGWKLDGVKRGGHIKGAVDFSANWLSVYSDRKDEVLEQALKTKRIDLDKNIVLYDAN
                     GKDALVVANYLSKKGYKYLYKYDIKQWADDENLPMERYKNYQMIVPAFIIKDILDGKI
                     PETFEDSKNIKMIEASWGKESYTKGHIPTSVHVNTDIIEPPPTWMLDNDDNLTKFALD
                     YGLTKDDTVIVSSSTPMASYRLAVILRYIGVKDVRVLNGGTNSWLSAGYELEFISNPK
                     HSCTNFGADIPVNSQLIVTTSELRQKLKEKNKFILVDNRTWDEHIGKVSGYTYYDKKG
                     RIPGALYGHSGSDSVSLEEYRNIDNTMRNKYEILEMWDKENIDVNKQLIFMCGSGWRA
                     AEVLTYANVIGVENTSLYSDGWMGWSLDNSNLIEVGEHK"
     gene            13375..14112
                     /locus_tag="KPD58_000226"
     CDS             13375..14112
                     /locus_tag="KPD58_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HBG0264017.1"
                     /translation="MKKVKLAISFFILMIIGMTISYADTSINTRYNRLSKALEISEAN
                     FKFYNVKINSVINHNLSNKEFHNIFIEIINNLKLDKKGINWNQKGSGDKIQLCATIKG
                     SDKEISIKGINKNNKESYIVVDILDNKVYKHKEVIYSNVENVLNKYSSQVDVSACIVG
                     EYTKRLQNNKYDDILQKILYNMSAKQIDEVKDVNFLSITAYSKVLNDYELEYLGEKIN
                     LNIGIKYNEDDGKTLIYIATPIIKLDY"
     gene            14133..15386
                     /gene="murA"
                     /locus_tag="KPD58_000227"
     CDS             14133..15386
                     /gene="murA"
                     /locus_tag="KPD58_000227"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBG0264018.1"
                     /translation="MAKIIVKKSNPLKGSVKIDGAKNAVLPIIAATLLANGKSTLNGV
                     PNLRDVHVISDLLRHVGAEVEYKENTLTVDASNIKTCEAPYELVRKMRASFLVMGPLL
                     ARFNSTKISMPGGCAIGTRPIDLHLKGFKALGAKIEMDHGFVEAATEKLVGNKLYLDF
                     PSVGATENIMMAASLAEGTTIIENAAEEPEIVDLANFLNEMGADVKGAGTNTIKIKGV
                     KELKGAEHNVIPDRIEAATYMVAAAMTKGDITVENVLMEHLKPVVAKLREAGCEITEM
                     DNSVRVVGPKVLKPIDIKTLPHPGFPTDVQAQFMAMLTVANGTGVVIETVFENRFMHV
                     AEFNRMGANIKIDGRSAVVNGVDELHGAAVNATDLRAGAALILCGLIAEGETQIGEIY
                     HIQRGYVDIDKKITALGGQIEIVED"
     gene            15473..16537
                     /gene="spoIID"
                     /locus_tag="KPD58_000228"
     CDS             15473..16537
                     /gene="spoIID"
                     /locus_tag="KPD58_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HBG0264019.1"
                     /translation="MKNPLVVLLGFVTCSVLVPSLITLVSYKNVELTEKPESPVSINK
                     TIKKSDIEDKGNKEEKNTVNYETVNKKAPIINVYNHITGKTEKMDMENYLCGVLAGEM
                     SSEFDIEALKAQSVAARTYVVYKQEHGKSSKHKNAVVCTDYKHCQEYKSYDTLKKLNG
                     EEWIKNKYSKIQEAVRGTKGQIITYNDKAILPLYFSTSSGKTENSEEVFSAKYPYLKS
                     VESPYDKYSPKFASTLKISNTDFVKSLRRAYSTIVIDVNNLSKQVSITKRSDAGTVEK
                     IKLGNKELTGKDIRTVFKLNSANFDIKFGEGYIDFVVKGYGHGVGMSQWGAEGMAEEG
                     YKYYDILSHYYTDTKIKDIY"
     gene            16603..17271
                     /locus_tag="KPD58_000229"
     CDS             16603..17271
                     /locus_tag="KPD58_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HBG0264020.1"
                     /translation="MKKKLLEKDGFYLSLFVCVCLLAVGGVWFTNNNVDKLASNKGIM
                     ENANKDSEEEIHLIEKDKKDAIPTATDSKQNLEKAKSKEENKSSTTKLNYIGDKVIRG
                     YSEKEPSYSKTLDVWETHKGVDISCTKGKEVKSLLNGIVVDVFDDEEYGQSVKIKSDN
                     NIVVVYSNLDKNVSVKKEQKVTEGQSLGTVGSTSQIESEEGIHVHLEAYSGEKSIDPM
                     SLIK"
     gene            17369..17629
                     /gene="spoIIID"
                     /locus_tag="KPD58_000230"
     CDS             17369..17629
                     /gene="spoIIID"
                     /locus_tag="KPD58_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HBG0264021.1"
                     /translation="MRSHIEERAIVVAKYILEKNTTVRQTAKTFGVSKSTIHKDVTER
                     LKEINPSLAKEVKNVLDKNKSERHIRGGLATKLKYEKEHKKM"
     gene            17826..18845
                     /locus_tag="KPD58_000231"
     CDS             17826..18845
                     /locus_tag="KPD58_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HBG0264022.1"
                     /translation="MAGADIGIDLGTANVLVYVSGKGIVLEEPSVVAIDKRTDSVLAV
                     GEEAKKMIGRTPGNIVAIRPLRDGVISDYDVTEKMLKSFIDKIVDKKGFGRFFMPRIM
                     VCVPTGVTEVEKRAVEDATRQAGAREVYIIEEPIAAAIGAGVDISQPNGNMIIDIGGG
                     TVDIAVISLGGAVVSESIKVGGDRFDDAIVKYMKKQHNLLIGERTAEKIKFEIGSAFK
                     REEEKYMKITGRNLITGLPSSITINSTEMLEALRECVEQIVVATHAVLEKTPPELAAD
                     IGDVGIIMTGGGSLLYGLDKIIEQRTGITVTIADEPLSCVAKGTGIALGSIDLLETGG
                     SFKRK"
     gene            18861..19289
                     /gene="fabZ"
                     /locus_tag="KPD58_000232"
     CDS             18861..19289
                     /gene="fabZ"
                     /locus_tag="KPD58_000232"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBG0264023.1"
                     /translation="MLNIDEIKKLIPHRYPFLLVDKITELEVGKRAVGIKNVTVNEPF
                     FQGHFPEYPLMPGVLIVEALAQVCGVAMMSVEENKGKLGVFAGIDKVRIKREVRPGDT
                     LTMEVEMTTLRKNIAKADAKAYVGEELVCKGELMFALVEK"
     gene            complement(19400..19909)
                     /gene="yyaC"
                     /locus_tag="KPD58_000233"
     CDS             complement(19400..19909)
                     /gene="yyaC"
                     /locus_tag="KPD58_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HBG0264024.1"
                     /translation="MYLAKVNYSDDDVIQMLSSVLKTIINENTIVVCIGTDRAIGDTL
                     GPLVGTILKNSNFKYPVYGTLDNPIHALNIYESLDTIKNTHIQGNFLAIDACLGSQSN
                     IGNIQIREGPILPGKGVGKKLPQIGNYSIVGIVDKIDENNKLSFNNIRLSFILDLAET
                     IALALLVST"
     regulatory      20098..20201
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            20338..21531
                     /locus_tag="KPD58_000234"
     CDS             20338..21531
                     /locus_tag="KPD58_000234"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBG0264025.1"
                     /translation="MARHLFTSESVTEGHPDKICDQISDSILDALLEKDPQSRVACET
                     TVTTGLVLVAGEISTSAYVDIPKLVRETVREIGYTRAKYGFDCDTCAVITSIDEQSGD
                     IAMGVDEGLESKTGEEIEEEIEKVGAGDQGIMFGFACNETPELMPLPISLAHKLSRRL
                     TEVRKTGLVDYLRPDGKTQVTVEYEGSKAVRVHTVLISAQHCETVSNDKIREDLINHV
                     IKEVIPAELLDEETKIYINPTGRFVIGGPQGDTGLTGRKIIIDTYGGYSRHGGGAFSG
                     KDPTKVDRSAAYAARYVAKNIVAAGLADKCEIELAYAIGIARPLSIFIDTFGTGKVSE
                     EKLVELVNKHFDLRPGAIIRDLDLRKPLYKKVAAYGHFGRTDIDLPWERTDKVEQLRK
                     DALGE"
     gene            21847..22944
                     /locus_tag="KPD58_000235"
     CDS             21847..22944
                     /locus_tag="KPD58_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264026.1"
                     /translation="MSGKVNIKTVISFAGAYVATVIGSGFATGQEILQFFTFYGYAGI
                     IGGIISMVLFSWFGAEVIDKGRELKLKEPIKIYQVYCGKYLGTFFEWFGPLFLFGTLV
                     IMIAGAGATLSEYYGLNPYVGRIGMAIVSLITVSLGLTRLSKILGNIGPIIIIFTLLV
                     GAISLFSNIDALSNAGNMVDSLNIKTATSNGYFSGVLYTCYNVIIVITFLTGMGASAV
                     NKKDAVWGGIVGGVALMAAAIMMNLALLSDIGNIYTKEIPALYLADKISPIIGILFSV
                     VLLLGIYTTAVPLLWSVTNRFVEDDHPKFKIITIVVSILACIGGLLPFDKLVGTLYPY
                     TGYMGILILLCMLYRRITKTEGYKENKSEIS"
     gene            23208..25427
                     /locus_tag="KPD58_000236"
     CDS             23208..25427
                     /locus_tag="KPD58_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HBG0264027.1"
                     /translation="MEKLEGMISEIVFKNEDNGYTIAHLVNENDEIVVVGCMPTLAIG
                     ESIEVEGKWVNHKIYGTQFEVNSFMPVTPSSLEGIYVYLSSGMIHGIGEKMAKRIIDK
                     FGVDTLEVIQNSPEKLQEVEGIGSKKVKQIVKSYEEDRELRNIIIQLSPFGITPNYCL
                     KIYKKYKSSAIEVINKNPYQLAEDIRGIGFKVADSIASKIGIDKNSKDRICQGILYTL
                     NKSLSNGHTYLPEHVLIQDSEKLLELNGEIIKECVMMLVYNQKIHIEKVNNENLIYLM
                     PYYLAENGVCSQIVKLSQYEFEDLKIDIDNEINVLEEDKKIKLAEKQILAVKESVNSG
                     VLIITGGPGTGKTTTINAIIDIFENNGKSVTLAAPTGRAAKRMSETSNKEAKTIHRLL
                     EMGFSTDDDLTFFKDEEDPINSDVIIVDEVSMVDIILMYNLLRAIKLGTRVILVGDSD
                     QLPSVGAGNVLKDMINSNIINVVKLNEIFRQAQESMIIVNAHKINNGEPLYLNTKGKD
                     FFFIRKSTNEEILNEIIGLVNERLPKFYKVDKLKDIQVLSSMRKGELGVTNLNIELQK
                     YLNKKEKFKVEESFSKRLFRVGDKVMQVKNNYTKKWETEDQKESGEGIYNGDIGYVYH
                     IDKDKKTIYILFDQTKIVSYLYDELDEIDHSFCTTIHKSQGSEFPVVVLPIAWAPPML
                     LSRNLLYTAVTRAKKLVVLVGDVKYLEYMIKNNRVNQRYSNLGYKLNKFKQEGLLIE"
     gene            25430..26212
                     /locus_tag="KPD58_000237"
     CDS             25430..26212
                     /locus_tag="KPD58_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HBG0264028.1"
                     /translation="MKICNDNFIKNFKNMINKCLDFIYPENISCIICDKSIKKTNTYS
                     ICKSCFKEMNFIQDGCMKCGKPIICHSIEKEFIEECSYCFNKDFYFDKSISCIEYNDV
                     TKKMVLGLKYNQKTFMAKYIAQIMKEKLYLENIKFDYILFVPLHKKRLNKRGFNQAQK
                     IAFNLSKIVNIPLLDCISRKKYTRMLYKLNKKERKEELKNVFVVKENVKLINNKNILL
                     IDDIFTTGLTTNEISKLLKLSGANKVFVLTLLTKANDNYIME"
     gene            26544..28457
                     /locus_tag="KPD58_000238"
     CDS             26544..28457
                     /locus_tag="KPD58_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG0264029.1"
                     /translation="MICLNKRQENLLTTLLLKEEFINVNKIANDFECSERTIRNDCKY
                     IDEWLSTFCNAYIKRKPNIGIKFSGDGTDKILVNQKLRGEVKRVNSELHKQVEILNLL
                     LCNNKKVTLNDLSNKLYINKNIVREEISKLSVALNNYNLNISTKKGSGIYIEGDEKDI
                     RIMLVSFLFKYIDKYRLNIDEIEYFHIVDIIIVKNIMLTIEEDMDIRLTDISFKQLML
                     FLLINIIRIRLGNSLKVSNTKKELKLKMLILDIEDELKSNLSIVLNYEEKLFIESLIL
                     GMNKQINSNNIKKSLYLNEELASYTKKIINLVSEESGINFNNDILLYEQLICHLNVTM
                     HQMKSNVYLENPLLDNIKTKFCFLFTVISSSIEIELKGKQITEDEIGYLTLHFQTSLE
                     RKYSKRESNKKASIVCPFSFGVSMLLKVKIEKRFDNIKIIETLREEDLKRDNFNKTID
                     FIIAFQEYENIQKPIFITTPLFTDEDENKLKEFTNKIKEKDTSYKLMNRLMMSDYLIR
                     ELEHDDIYEAITYLTNLLIEKHYAEKEYLQSIITREKSYPTNVGKGILFPHGDMKYIK
                     QSVLCFARLKTPIKIRNGKDIKIILLLAYKKDDDRKVFRELFKEISNLTEDENMLDIL
                     SKCDIEKVKDILI"
     gene            28598..28906
                     /locus_tag="KPD58_000239"
     CDS             28598..28906
                     /locus_tag="KPD58_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG0264030.1"
                     /translation="MEEIVLKIIIHAGNAKSMLYEALDYAKENDFKKADELIENANEE
                     ILKAHKVQTELIQKEAGGDKSDISILLIHSQDHLMTCMSERNLIKEMIMLRKEIQKLK
                     "
     gene            28929..29255
                     /locus_tag="KPD58_000240"
     CDS             28929..29255
                     /locus_tag="KPD58_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0264031.1"
                     /translation="MKRKVYLFCSFGMSTSLLADKMQKVADEHNLPIEVEAFPIAEID
                     KIVEEKNPDCILLGPQVKYMLKELKPKFEAQGKLIDIINEVDYGTMNGEKVLKLAIKL
                     IKSKQA"
     gene            29294..30628
                     /gene="celB"
                     /locus_tag="KPD58_000241"
     CDS             29294..30628
                     /gene="celB"
                     /locus_tag="KPD58_000241"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBG0264032.1"
                     /translation="MNRLERILMPLAEKIGKNKLLIAIRDGFLVSSPLLIIGSLFLLV
                     ANFPIKGWNEFVGQIFGPDWAVKLQQPTVATFEVMTLLAVLGIGYSYAKQIDVDPIAS
                     AAVAIVAFFIITPFVIPYTPEGTESVYMVTGIPLGWMGSKGMFVGMITAITSVKLFAA
                     VVKKGWTLKMPDGVPPTVSKSFAALIPSAIVMVVFFLVKIAFEATPYGSIHEFIFKFL
                     QMPLLKLGNSLGAMAIAYIFLHLFWFFGINGSSVVGAVYNPILKILSAENLAAFQAGS
                     KIPNIITGQFQDMFATFGGAGSTLSLVIAMLLICKSKRIKSMGKLSILPGIFGINEPL
                     IFGLPIMLNPILLIPFAIVPTINIIIAYFCMSAGLVPLTNGVQLPWTTPIIFSGFLTT
                     GWQGAVLQLVLLILGIFMYIPFIKMLDKQYLREENETVEEEDDDISFDDLVL"
     gene            30639..31160
                     /locus_tag="KPD58_000242"
     CDS             30639..31160
                     /locus_tag="KPD58_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HBG0264033.1"
                     /translation="MKKIVMILDQIQAGAGGKEKSNIPPAGKSSPLGPGVMMEQFLNE
                     SKVIATLFCGDEFFVNNQEEVTSKMIAMVKKLNPDVVICGPSFNYENFSKMSAILSKN
                     INDKTDIPAFAAMSEENIDVINEYKNDICIVKTPKKGGIGLNDSLNNICKLAKAIANK
                     EDITLMKEEFCYQ"
     gene            31253..32212
                     /locus_tag="KPD58_000243"
     CDS             31253..32212
                     /locus_tag="KPD58_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HBG0264034.1"
                     /translation="MWGMIATWRMALEGITEASEHLEKGMEAGDAVELAVRRVEDYPF
                     YKSVGYGGLPNENCEVELDAAFMDGNTLDIGAVAGIRDYSNPVSIARKLSYEKVNNFL
                     VGIGAEDYAHKNGFERKNMLTDRAKLHYKKRKKETLDKGLSPYAGHDTVGMISLDKTG
                     KMCAATSTSGLFMKKRGRIGDSALSGSGFYVDSSIGGATATGLGEDLMKGCISYEIVR
                     LMKEGKHPQEACDIAVNELDKQLIERRGKAGDLSLVAMNNKGEWGVATNIADFTFSVV
                     TKEESPTVYISNRVDGKTIHKVISNEWMEAYEARIKAPIEVSE"
     gene            32261..33313
                     /locus_tag="KPD58_000244"
     CDS             32261..33313
                     /locus_tag="KPD58_000244"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HBG0264035.1"
                     /translation="MNIISNKIDELKEDLLSDIIDIVKIPSVKGESENGFPFGEKVGE
                     ALNKALEISEKLGFKVRNLDNYIGYAEHGDSDDYVCVIGHVDVVHEGDGWKHQPYKGE
                     ETNGRIYGRGVLDNKGPIMSALYGLYAIKELNLKLDKRVRIIFGTNEESGFEDIPYYL
                     EKEKAPIMGFTPDCKYPVVYGEKGMAKIRIKSKINYEEDVYLGFIENMSENVLVTYKE
                     LNIENSDTILDIKVKYDFSYKLKDVLDEIKASFPNSIDIEVISNFNPVYFDKESNLVK
                     KLQLAYERVTSLDGTPVTTNGGTYAKVMPNIVPFGPSFPGQKGIAHNPDEYMDIEDII
                     LNAKIFANAIYELAKE"
     gene            33316..33990
                     /locus_tag="KPD58_000245"
     CDS             33316..33990
                     /locus_tag="KPD58_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBG0264036.1"
                     /translation="MLEIIGMSVEDAKIIEDCGADRIELVSALTEGGLTPSFGLIESV
                     VNSVKIPVNVMIRHHAKSFVYSKEDISIMQKDIGVVKEIGANGVVFGVLDKNNNIDEK
                     NLNVLLKCCDNLDVTFHRAIDESNTIDSVKILKDYDKITNILTSGGKGSIVDNIQMIK
                     NMMLSSNHIKILLGGGLNFNNIEKIKELTKASNFHFGTAIRINNSPFEDIDRQKLKQL
                     VNIISR"
     gene            34543..35094
                     /gene="raiA"
                     /locus_tag="KPD58_000246"
     CDS             34543..35094
                     /gene="raiA"
                     /locus_tag="KPD58_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBG0264037.1"
                     /translation="MNIIISGKQIELTDAIKNKIETKLSKLDNYIHPDTDVKVTVSAR
                     KARQKIEVTIATVNGPIIRAEDMQENLYTAIDVVYDKLSKQLKKYKKRLQDKHKDNKS
                     IRFQDAEINLADEQEDYGDENQELVIQRRKKFSVKPMSEEEAVLQMELIGHDFYMFKN
                     IDSDEIAVVYKRNNGGYGIIEHE"
     gene            35226..37901
                     /gene="secA"
                     /locus_tag="KPD58_000247"
     CDS             35226..37901
                     /gene="secA"
                     /locus_tag="KPD58_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBG0264038.1"
                     /translation="MSFMDNLFNMADKKELKKFNKTVDIIDSLEPKFESMADSELKNM
                     TNIFKERLANGESIDDILPEAFAVVREVSKRVLGLRHYRVQMIGGIVLHQGRIAEMKT
                     GEGKTLVATAPVYLNALTGKGVHVVTVNDYLAKRDRDQMAKIYEFLGMSVGVIIHGQN
                     PKVRKEQYDCDITYGTNNEYGFDYLKDNMVIHKEQRVQRGLNYAIVDEVDSILIDEAR
                     TPLIISGPGDKSTHLYSDANTFVLTLKPDDYELEEKDKAVSLTASGIQKAEVYFNVDN
                     ITDISHTELYHHINQALRAHVIMKKDVDYVAKDGEIVIVDEFTGRLMFGRRYSEGLHQ
                     AIEAKEGLKIQRESKTLATVTFQNYFRMYKKLSGMTGTAKTEEEEFKAIYKMDVFQVP
                     TNKLMIREDLPDCVYKSEIGKFNAVAQEIIERHKVNQPILVGTVSIEKSELLSQILKK
                     KGIKHEVLNAKHHDKEAEIIAQAGRLGAVTIATNMAGRGTDIVLGGNPDFLTKREMRR
                     NGFKEEIVNRVDTPIEGIPVKGNEILFEAREEYEKLFEKFKQQTQEEQKQVVEAGGLA
                     IIGTERHESRRIDNQLRGRAGRQGDPGSSRFYIGLDDDLMRLFGSDRISGIVDKIGLE
                     EDMPIEHRILSKSIEGAQKKVEGKNFGIRKHVLQYDDVMNKQREIIYAERKRVLEGED
                     LQEQIQSMTHSIIEEAVTLYTQDKGFDEEGFKEHMYNLFLPKGSIEIPEIEKLNPVEI
                     TEKVYEIAMKIYTSKEEQVGYERMREVERVILLQAVDNHWIDHIDAMDQLRQGIGLRA
                     VGQQDPVIAYKMEGFDMFDEMNKHIKEDTVRYLFNITIETPVERKAVVDVENLSSPSD
                     GTLPTSKTVKKDEKVGRNDLCPCGSGKKYKNCCGR"
     gene            37916..39023
                     /gene="prfB"
                     /locus_tag="KPD58_000248"
     CDS             join(37916..37990,37992..39023)
                     /gene="prfB"
                     /locus_tag="KPD58_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153309863.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBG0264039.1"
                     /translation="MLKLQEYRHSMEQMASNLEELRVSLDIASLMKNIQINEEKMNQQ
                     DFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIGLEEDNDSVERDIEKS
                     IESMEEKLSEMKIKTLLNGEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKG
                     YKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHRLVRISPFDPSGKRHTSFA
                     SIDVTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQN
                     ERSQHLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQIRSYVFQPYKLV
                     KDHRTNAEFGNVDSVMNGNIDLFINEYLKMNKIV"
     gene            39167..41308
                     /locus_tag="KPD58_000249"
     CDS             39167..41308
                     /locus_tag="KPD58_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBG0264040.1"
                     /translation="MDINQILKKEFNLRDEQINNTLKLIDEGNTIPFIARYRKEMTGE
                     MSDVTLREFYEKLMYLRNLQSRKDDVVRLIDEQGKLTDEITQNIEKAKTLQEVEDIYA
                     PYKQKKRTRATIAKEKGLENLALSILENNLDNIEIEAKNYLDEEKEVLSIEDALKGAR
                     DIIAELVSDDAKIRKYIRELALREGMIVSKSATDEKSVYDMYYDYSEAVKSMAPHRVL
                     AINRGEKESFLKVKLEINNDKVLNYIINEYVNDKNFKNKEEIVSSIEDSYKRLIFPSI
                     EREIRNHLTEIAQERAISVFGKNVKSLLLQPPVKDKVVMGFDPAFRTGCKIAVVDKNG
                     KLLDYTTVYPTDPQNDVEGAKKVLKGLIEKYDIDIISIGNGTASRESETFVSEMIKEI
                     DSEVQYVIVSEAGASVYSASELANEEHPDINVSIRGAISIARRLQDPLAELVKIDPKS
                     IGVGQYQHDLNKKRLEEVLDGVVEDSVNSVGVDLNTASYSLLEHVAGISKAIAKNIIA
                     YREENGDFTSRAQLKKVKRLGPQAFTQCAGFMRILEGKNPLDNTGVHPESYDICKKMI
                     EIIGYSLDDVKNKNIGEIDEKIKEIGLRELSEKLEVGQVTLKDIIAEIKKPGRDPREE
                     GIKPILRTDVLKIEDIQEGMTLKGTIRNVVDFGAFVDIGIKNDGLVHKSEMSNSFVKD
                     PMSIVTVGDIVDVKVIGIDLNKKRVALSMKK"
     gene            41456..42622
                     /locus_tag="KPD58_000250"
     CDS             41456..42622
                     /locus_tag="KPD58_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0264041.1"
                     /translation="MIFIKNGKINTITNGIIHGDILIDEGKIIEIGEDLIAPLDVEVI
                     DASNKLVFPGFIDAHTHLGLWEDGIGFEGADGNEETDPITPQLNPIDGINPMDRTFKE
                     AFEGGITSVCTTPGSANVMGGQCIAIKTCGKRIDKMVIKNPVASKIAFGENPKSCYGQ
                     DDKSPQTRMAIAALLRENLKKAEEYLEDIDMYESHDDEDCEKPEYDIKMESLIPVLRR
                     EIPFKAHAHRADDMFTAIRIAKEFNLKLTLDHCTEGHLIVDELVEEGFPVIVGPSLSE
                     RSKFELRNLTFNTAGILSNAGLDVCIMTDHPVIPVQYLPICAGIAVKHGMKEEKAIES
                     ITINPAKALGIEDRVGSIEVGKDADLVIWDNSPLEIQSNVLYTIINGKVVYEKK"
     regulatory      42682..42799
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            42924..43529
                     /locus_tag="KPD58_000251"
     CDS             42924..43529
                     /locus_tag="KPD58_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG0264042.1"
                     /translation="MQNTAKRGNMISTRTLVVMSILSAISYLLMFIQAPIPGIFPDFL
                     KIDLSDIPAIFGGMSLGPLVGFGIVVVKNVLQAITATTTGGIGEFANILIGGSYVMIL
                     CYSYKRSGDLKGVLIGFVLGTISMTIIGSLVNYFVMLPLYGQLMGLDAIIGLGSAINP
                     QVHDLFTFVIWMIAPFNVLKAVIISLVTLPLYKKMGNIIKK"
     gene            43858..44898
                     /locus_tag="KPD58_000252"
     CDS             43858..44898
                     /locus_tag="KPD58_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBG0264043.1"
                     /translation="MHLSKKIKKLFLDDKGYYFNSNWKDIHIGIYDTKLGDSLKYITN
                     EYIENMERVKYDYRELKNIENQTDGMCILAIRQNYKALEYVKNQTEELCLEAIKYNYK
                     ALEYVKEQTEYLCLEAIKKDCNALKYVRNKTEGLIIKAISHSSNIDVVSILKALETQT
                     RRICLEAIKKDGRCLAYVREQSEELCIEAIKQNYKALKYVKNQTEKMCIESVRQNGMA
                     LQYVNKQTDKICIEAVKQDGRSLQFVNNKTEEICINAIRYLNKKYNIKDVLSYIDKYT
                     EDICIEIVRQNGKMLMYIKNQTEKMCIEAVKENYKSLKYVKEQSERICKEALKQNHKA
                     KEYVKIAIDDCI"
     gene            45037..45489
                     /gene="tsaE"
                     /locus_tag="KPD58_000253"
     CDS             45037..45489
                     /gene="tsaE"
                     /locus_tag="KPD58_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBG0264044.1"
                     /translation="MAKIYLENENKTREIGYKLGKLLKEGSVICLVGDLGAGKTTMTQ
                     SLADSLGIEDYITSPTFTIINEYEGKIPLYHFDVYRIGSSDEMYDIGYDEYVNSNGIC
                     IIEWANLIEDILPKEYLNIELRYKDEGREMILTPKGEFYKEIVEELIK"
     gene            45489..46205
                     /gene="tsaB"
                     /locus_tag="KPD58_000254"
     CDS             45489..46205
                     /gene="tsaB"
                     /locus_tag="KPD58_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBG0264045.1"
                     /translation="MKILGMDTSSMAASVAVVEDDNLICEFTVNNKKTHSQKLMPMIE
                     NMLSMSDLSIKDMDLLAVCIGPGSFTGLRIGMATVKAMAHVNNIPIIAVNSLESLANN
                     INFCDRKICCILDAQKNQVYSAKYKFENGEMIELDGVDVVEFDALVDEIVSTNEEFII
                     VGEAVYKYKDKLENIKNIKIPSPANNVSNAGSLCSLALNKYNKNIDVHTCYTINPMYI
                     RKSQAEVQYDEKMKRLNNGR"
     gene            46195..46671
                     /gene="rimI"
                     /locus_tag="KPD58_000255"
     CDS             46195..46671
                     /gene="rimI"
                     /locus_tag="KPD58_000255"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBG0264046.1"
                     /translation="MEDNTKLIVDDIKIEEMTTEDIDEVFEVEKNCFEDYWSKESFRK
                     ELSNEVARYIVAKLNGKIVGYVGIWLILDEGHINNVAVHSDYRGKKIGDKLIKGIVDL
                     CKDNNIASMTLEVRASNKIAQNLYRKYGFKMGGIRKEYYSDNKEDAIIMWNQLKEV"
     gene            46677..47693
                     /gene="tsaD"
                     /locus_tag="KPD58_000256"
     CDS             46677..47693
                     /gene="tsaD"
                     /locus_tag="KPD58_000256"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBG0264047.1"
                     /translation="MSDIITLAIESSCDETAASVLKNGREVLSNIISTQIETHKKFGG
                     VVPEVASRKHVENIDIVVQEALDKANIGFNDIDHIAVTYGPGLVGALLVGLSYAKALA
                     YTLNIPLVGVNHIEGHLSANYIEHKDLKPPFITLIVSGGHTHLVEVKDYGKYEILGKT
                     RDDASGEAFDKISRAMNLGYPGGPIIDNLAKNGNKHAIEFPRAYLEEDSYDFSFSGLK
                     SSVLNYLNGKRMKNEEIVVEDVAASFQEAVVEVLSTKALKAVKDKGYNIITLSGGVAS
                     NSGLRAKITELAKDNGITVKYPPLILCTDNAAMIGCAGYYNFINGKTHDMSLNAVPNL
                     KINQ"
     gene            47990..50698
                     /gene="hpdB"
                     /locus_tag="KPD58_000257"
     CDS             47990..50698
                     /gene="hpdB"
                     /locus_tag="KPD58_000257"
                     /EC_number="4.1.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase large
                     subunit"
                     /protein_id="HBG0264048.1"
                     /translation="MSQSKEDKIRSILEAKNIKSNFQNKENLSEFNEKKASKRAEDLL
                     DVYYNTLSTADMEFPYWYNREYRKSDGDIPVVRRAKALKAAFSHMTPNIIPGEKIVMQ
                     KTRHYRGSFPMPWVSESFFVAQGEQMREEAKKLASNTADELTKFGSGGGNVTESFGNV
                     VSIAGKFGMRKEEVPVLVKMAKEWVGKSVEDLGFHYEKMMPDYDLKENLMSTLICMFD
                     SGYTLPQGREVINYFYPLNYGLDGIIEMAKECKKAVAGNASGDGLIGMDRLYFYEAVI
                     QVIEGLQTWILNYAKHAKYLESIETDLEAKKEYSDLVQILEHIAHKQPRTFREALQLT
                     YTIHIASVNEDAISGMSIGRFGQILYPWYEQDIEKGLITKEEVIELLELYRIKITCID
                     CFASAGVNGGVLSGNTFNTLSIGGLKEDGSTGANELEELLLEASMRCRTPQPSLTMLY
                     DEKLPEDFLMKAAECTKLGSGYPAWVNNSNGTTFMMKQFADEGMTVEEARAFALGGCL
                     ETSPGCWKQLTLNGKTYSIAGGAGQSAGSGVHFIANPKILELVLMNGKDYRMNIQVFE
                     PHNKPLDTYEEVIEVFKDYYKQAINVLERANNIELDIWRKFDTSIINSLLKPDCLDKG
                     QHIGNMGYRYNATLNVETCGTVTMVNSFAALKKLVYDDKAFTIEEMKDAILNNFGFKD
                     ALEVGNYSMADQVKVDKTGKYDAIYKACLDAPKYGNNDLYADNILKNYEVWLSKVCEE
                     AQSLYAKKMYPCQISVSTHGPQGAATLATPDGRLSGTTYSDGSVSAYAGTDKNGVYAL
                     FESATIWDQAVVQNSQMNLKLHPTTIKGQQGTKKLLDLTRSYLRKGGFHIQYNVVDSE
                     TLKDAQKNPDNYRQLMVRVAGFTQYWCELGKPIQDEVIARTEYEGV"
     gene            50702..50959
                     /gene="hpdC"
                     /locus_tag="KPD58_000258"
     CDS             50702..50959
                     /gene="hpdC"
                     /locus_tag="KPD58_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase small
                     subunit"
                     /protein_id="HBG0264049.1"
                     /translation="MRKHSDCMNFCAVDATKGICRLSKQMINLDDAACPEIKVMPKCK
                     NCKNFVEANDEGIGKCVGLEKEDWVYSTLNAITCEGHVFNE"
     gene            50952..51902
                     /gene="hpdA"
                     /locus_tag="KPD58_000259"
     CDS             50952..51902
                     /gene="hpdA"
                     /locus_tag="KPD58_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase activase"
                     /protein_id="HBG0264050.1"
                     /translation="MSSQKQLEGMIFDVQSFSVHDGPGCRTTVFLNGCPLSCKWCANP
                     ESWTVRPHMMFSELSCQYENGCTVCHGKCKNGALSFNLDNKPVIDWNICKDCESFECV
                     NSCYYNAFKLCAKPYTVDELVQVIKRDSNNWRSNGGVTFSGGEPLLQHEFLHEVLLKC
                     HEVNIHTAIETSACVSNEVFNKIFNDIDFAFIDIKHMDREKHKEQTGVYNDLILENIS
                     NLANSDWNGRLVLRVPVISGFNDSDENISDIISFMHKNNLVEINLLPFHRLGESKWIQ
                     LGKEYEYSDKGDVDEGHLEELQDIFLDNGIACYVGHETAF"
     gene            52217..53179
                     /locus_tag="KPD58_000260"
     CDS             52217..53179
                     /locus_tag="KPD58_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG0264051.1"
                     /translation="MNKSWSNLIIKNKDRKVRSGWIIIAVMATFYILQYYLSMILVEI
                     IRKILVATGDINLKTNYFSEYANWLNDVGLQIVFQILTDSLMIIIPIIVWRFIMKNPI
                     SEMGLHSYRIKKKEGYVGMLLGIFNCTVIFIITITIGGGHVTSWVPKISPLTVSWILV
                     FILVAFAEEILNRGFFMAVLRRCKNIYFIMIVPSVIFGLIHIWNPDVTFLSVINIIII
                     GILFSYMFIKSSNIWMCIGYHFAWNVFQGIIYGMPVSGLQVPGIITTQITHGNLLNGG
                     MFGIEGGILTTFVNLLSFIFVWYYYRDSKYDFVSDKVNVTNIKN"
     gene            complement(53278..54081)
                     /locus_tag="KPD58_000261"
     CDS             complement(53278..54081)
                     /locus_tag="KPD58_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG0264052.1"
                     /translation="MMILPLSSVAIAKFYTEGLNNDMYEFYSLIILFFLIYSLLIVLK
                     LLNLINNNEFIIINLILTFISSLSIIIYSSNKKNLYPVKNIKLGSLLIIYFMISVIVP
                     TSFILILQGAQPNYSGIIKFIFTPITSLTTIYIFFSEEYAWRGFLQNIFFDKFGKKLG
                     VIILGMCWSLWHLPLIFTLYTPEAPILGIILRSIHIVGISIFLGYLYIKTKNIWFCAI
                     IHVLNNSAFLITNSKESTITYHDILIVSMVVLIFYVPFLFTKEYKNNLE"
BASE COUNT        21869 a         5781 c        10278 g        16323 t
ORIGIN      
        1 agtagcgtat attttttaca aaatatttat gtttataaaa gatacaagag cagaacaagt
       61 gtttaaaggt ataatatttc ttttattagc aactcaactt agtaacacct ttaaattgca
      121 taccgtttat tggatatctc ttaaagcttt agattatgga gttatagctg ctcttataat
      181 ttttcaacca gaatttagag caggacttga acatatagga agagcaaagt ttaatttatt
      241 tggaaagaat gtaaatactt ctgaagaaac tctcaataga aatatagaag aaattgtaga
      301 agctttatat tcactttcta gacaaaaaat aggagctctt atcataatgg agagagaaac
      361 tagaataagt gatataataa atacaggaac tattattgat gctgaaattt ctaggcaact
      421 attaataaat atatttatac caaatacacc attacacgat ggagctgttg taattagaga
      481 ttctaaggta aaagcagctg catgtttttt gccattgaca gaaagtaagg atttaagtaa
      541 agatttgggc actagacata gagcaggaat aggggtaagt gaggtgtctg actgtataac
      601 tctgattgta tcagaggaaa caggtggcgt atcaattgct aaagcaggta agttatatag
      661 agatatttct agagaaagaa tgatgaatat attacgtagt aatttaaaaa caaatacaga
      721 aacaagaagt tttttcaaag gtggtatatt taaatgataa atagactaaa aaataatacc
      781 aaaataaaaa taatatcatt tttaagtgca attgcattat ggatgtatgt tatggctgta
      841 gttgaccctg aagaaactaa agggtttgaa gatatacctg tatctataag taatatgagt
      901 gatttaaagg ataaaaatct tgttatatat ccagacgaaa aattaacagc agatgtatat
      961 ataaaaggta gtttatcaaa ggtacaaagt gttaaaaaag aagatataca tgtatatgga
     1021 accattgaag acccaataca aggacagaag gaagtatatt taaaagcaaa tattcctcaa
     1081 ggtgttactc tagaatttaa gcatgatgtt ttagtagtaa atcttgaaaa aaacataaaa
     1141 gaaaaaagag atataaaggt agaagtaagt ggaaattcaa aaagaaatat aaataaaatt
     1201 gaacttagta aggaatttgt cgatgtatca ggtccaagaa ctctagttaa agaagtcaaa
     1261 agtgtaagag ctaatctaga tgttggtaat gagacagatg atttttctaa aaaattgcaa
     1321 ttaataccat taaatagtga tggaactaag gttgaaggag taacattaag ttcatccttt
     1381 gtgactgcaa agatagagct attgaaggaa aaaattgtcc caataagagt aagtctaaat
     1441 gataataaag ataatgatgt actgaaaaac tataaattaa gtaaggaaca agttacaata
     1501 aaaggaaaga aagatgatat agacaaaata agttatatta agacaaaacc tattgactta
     1561 gctgatttgg ctaatggtga agaaaaagaa gttagcttag aaattccaga gggtataact
     1621 cttgaggata gctctataac aattaaatta aatgatacaa aacaattgag tgcagaattt
     1681 ttatataatg cgggagatat tgaattaaga aatatcccaa caaatatgga taatccaaat
     1741 gtaatttctg atgtagatat aaaagttgtt gtagagtcaa cggaggattt gagtaagtta
     1801 aataaaaaag atatttcatt atatattgat ttatcacaag gtcctgagga agatagtaaa
     1861 tataaaataa aatatgaaac aacatatcaa tttaaaaaaa taaatataga acctaatatt
     1921 gttgaagtgg aatgaacaaa tatttcgaat atttagttgt tacaagatgc gacaaaaaaa
     1981 cctataaaat taagtttgtt aattttatgt ttttgttgca tcttgtttat tttagttata
     2041 ttataaaata aggacaatga gatatttgtt aaatctaaaa agcgctagat atatgaaaaa
     2101 agactgataa atagatttaa ataaatattt tgaattaaat atttcaatta tctctaggaa
     2161 aacgttgctg aacgagttat aatttaattg agatataaaa agttctattt caaaaaattc
     2221 aagatgaggt ggaaatgcat gagaagtttt gaagaagtaa ttaagtttgc aaaagaaaga
     2281 ggacctaaaa ctatatcagt agcatgttgc caagataaag aagttttaat ggcagttgaa
     2341 atggctagaa aagaaaaaat agcaaatgcc attttagtag gagatataga aaagactaaa
     2401 gaaattgcaa aaagcataga catggatatc gaaaattatg aactgataga tataaaagat
     2461 ttagcagaag catctctaaa atctgttgaa ttagtttcac aaggaaaagc cgacatggta
     2521 atgaaaggct tagtagacac atcaataata ctaaaagcag ttttaaataa agaagtaggt
     2581 cttagaactg gaaatgtatt aagtcacgta gcagtatttg atgtagaggg atatgataga
     2641 ttatttttcg taactgacgc agctatgaac ttagctcctg atacaaatac taaaaagcaa
     2701 atcatagaaa atgcttgcac agtagcacat tcattagata taagtgaacc aaaagttgct
     2761 gcaatatgcg caaaagaaaa agtaaatcca aaaatgaaag atacagttga agctaaagaa
     2821 ctagaagaaa tgtatgaaag aggagaaatc aaaggttgta tggttggtgg accttttgca
     2881 attgataatg cagtatcttt agaagcagct aaacataaag gtataaatca tcctgtagca
     2941 ggaagagctg atatattatt agccccagat attgaaggtg gtaacatatt atataaagct
     3001 ttggtattct tctcaaaatc aaaaaatgca ggagttatag ttggggctaa agcaccaata
     3061 atattaactt ctagagcaga cagtgaagaa actaaactaa actcaatagc tttaggtgtt
     3121 ttaatggcag caaaggcata attttaaggg ggaaataggc aaatgagcaa aatatttaaa
     3181 atcttaacaa taaatcctgg ttcgacatca actaaaatag ctgtatttga taatgaggat
     3241 ttagtatttg aaaaaacttt aagacattct tcagaagaaa taggaaaata tgagaaggtg
     3301 tctgaccaat ttgaatttcg taaacaagta atagaagaag ctctaaaaga aggtggagta
     3361 aaaacatctg aattagatgc tgtagtaggt agaggaggac ttcttaaacc tataaaaggt
     3421 ggtacttatt cagtaagtgc tgctatgatt gaagatttaa aagtgggagt tttaggagaa
     3481 cacgcttcaa acctaggtgg aataatagca aaacaaatag gtgaagaagt aaatgttcct
     3541 tcatacatag tagaccctgt tgttgtagat gaattagaag atgttgctag aatttctggt
     3601 atgcctgaaa taagtagagc aagtgtagta catgctttaa atcaaaaggc aatagcaaga
     3661 agatatgcta gagaaataaa caagaaatat gaagatataa atcttatagt tgcacacatg
     3721 ggtggaggag tttctgttgg agctcataaa aatggtaaaa tagtagatgt tgcaaacgca
     3781 ttagatggag aaggaccttt ctctccagaa agaagtggtg gattaccagt aggtgcatta
     3841 gtaaaaatgt gctttagtgg aaaatatact caagatgaaa ttaaaaagaa aataaaaggt
     3901 aatggcggat tagttgcata cttaaacact aatgatgcta gagaagttga agaaagaatt
     3961 gaagctggtg atgaaaaagc taaattagta tatgaagcta tggcatatca aatctctaaa
     4021 gaaataggag ctagtgctgc agttcttaag ggagatgtaa aagcaatatt attaactggt
     4081 ggaatcgcat attcaaaaat gtttacagaa atgattgcag atagagttaa atttatagca
     4141 gatgtaaaag tttatccagg tgaagatgaa atgattgcat tagctcaagg tggacttaga
     4201 gttttaactg gtgaagaaga ggctcaagtt tatgataact aatgataaaa gaataagaat
     4261 cataactggt cactatggaa gtggtaaaag tgaatttgcg atgaactatg ttgttaagtt
     4321 aagagatatg gtttctggca aagttgctat agccgattta gatgtggtaa atgtatactt
     4381 tagaactaga gaaaagaaag agttaatgaa atccttaggt atacaaccaa tagatagctc
     4441 aattaatgct ccaacattag atttaccagc agtatcagca gaggtaatgt ctcctatggt
     4501 agaccattca tacaattctg ttatagattt aggtggagat aatgtaggtg ctagggttat
     4561 aggaagattt agtcatttgt taaaagaagg cgattatgat atgctttttg taataaatgc
     4621 aaatagggag aaaactcaaa cttctgaaga agttatccaa tatataaaag aaatagaaaa
     4681 atcttctaaa ttaaaggtaa caggtctaat taataatact catcttatta ggtttactac
     4741 tattgatgat gttttaagag gtcagaaagt tgcaaaagaa gtttctgaaa aatgtaatat
     4801 accgataaga tatgttgcat gtttagaaaa tcttgttgaa cagttaccaa aagatttaga
     4861 aggagaaatt tttcctataa aattatatat gagagaagac tggatgtagt ttgagctcaa
     4921 atatatagaa agtttaagga ggcttaggta aatggctaaa ggaaaagtat cttttaatca
     4981 agaacgttgt aaaggttgtg gattgtgtgt tgaagcatgt ccagtaaaaa tcatacaatt
     5041 agactcaaat gtaataaaca aaaaagggta taacccagca actgtttttg aaatggaaaa
     5101 atgcatagga tgtgcaagtt gtgcaactat gtgtccagat gtagttataa cagtagaaag
     5161 agattaattt aattaatatt taagggggaa ttttaatggc taagatactt atgaaaggta
     5221 acgaagcatt tggaaaagca gctatagaag ctggttgcaa atatttcttc ggttacccaa
     5281 taactccaca aagtgaatta ccagaatatt tatcaagaga gttacctaaa ataggtggag
     5341 cgttcgttca agctgaatca gaagtttctg cgataaacat ggtttatggt ggagcaggtg
     5401 caggagcaag agttatgact tcttcatctt caccaggagt tgctttaaag caagaaggta
     5461 taacatatgc agtaggagca gaagttcctt gtgttgtact taatgtaatg agaggtggtc
     5521 caggacttgg aagtatacaa ccttcacaag ctgactactt tatgtctaca agaggtggag
     5581 gaaatggaga ctacagaact ccagtattcg caccagctac agttcaagaa gctgttgata
     5641 tgataatgga agcttttgac gttgcagatt attacagatc accagttatg gtagttgcag
     5701 atggtatgat aggtcaaatg atggagccag ttgaatttag agctcctgag aagaaaagag
     5761 aattgcctcc taaagattgg gcaactgttg gaactaaagg aaaaagaaaa cctaatgtaa
     5821 taaactctct atacttagag ccagaagtac tagaagacca ttgttggcat ttacaagaaa
     5881 aatttgatgc aatggaaaag aatgaagtac aatacgaaat gtacaaaaca gaagatgctg
     5941 aatttgtatt tgcagcttat ggaactactt caagagtagt aaaaagcgca attgatatat
     6001 taagagaaga aggaataaaa gcaggtctta taagacctaa agtattatgg ccattcccat
     6061 tcgaagcatt caaccaaatt cctaatgcta gaaacatatt gactgttgaa atgagtatgg
     6121 gacaaatggt ggaagatgtt aaaatggcag tagaaggtaa attaccagtt tacttccatg
     6181 gaagaccagg aggaatgact ccaacaccag cagaaatagt tgaaaaagct aaaaaaatta
     6241 ttgccggaga attggttgca ggaggtgcta gataatggct gttgtattta agaaaactga
     6301 aggattacaa gatactcaaa ctcactactg tccaggatgt acacatggta taatccatag
     6361 attagttggt gaagtattag aagaactagg agtattaggt gatgctgtag gtgttgttcc
     6421 agttggatgt tcagttttag gatataaata ttttaactgt gacactcaag aagctgctca
     6481 tggtagagcg ccagctgctg ctacaggaat aaaaagagtt catcctgaaa atacagtatt
     6541 cacttatcaa ggtgatggag acttagcttc aattggtact gctgaaatag ttcatgcagc
     6601 tgctagagga gaaaaaataa ctactatatt tgtaaacaat acaacttatg gtatgactgg
     6661 tggacaaatg gctccaacta cattagttgg acaaagagct actactgctc aatcaggaag
     6721 aaatgctgaa actcaaggtt atccaataag agtaagtgaa atgttagcta cattaacagg
     6781 agctgtattt gtagaaagag tagcagttga tactccagct catgttagac aagctaaaaa
     6841 agctataaag aaagcattcc aagttcaaca agcaggatta ggatttggaa tagtagaagt
     6901 attatctact tgtcctacaa actggggact agctccaaat gatgctttac aatggttaag
     6961 agacaacatg ataccatact atccattagg aaatttcaaa aatgttgaag ttgaggaggt
     7021 gaagtaaatg tctacagcta gagtaatatg tgcaggcttc ggaggccaag gtgttatgtc
     7081 tatgggacaa ttacttactt atgcaggaat gctagaagga aaagaagttt cttggttacc
     7141 atcttatgga ccagaaatgc gtggaggtac agcaaactgt gcagtaactg tatctaatga
     7201 gccagttggt tctccactta taacagatga tgcaactgca gctatattat taaatatacc
     7261 agcatttgaa aaattcaaag atgatgttat tcctggtgga aaaataatag taaatagttc
     7321 tttaataaaa gaaaaagttg atagaacaga tgtagatgtt tattatatac cagcaaatga
     7381 attagcagct gaattaggaa atgataaagt tgctaacatg ataatgttag gcgcttactt
     7441 aaaagtatct gatactgtag atatagaatc tgtactagaa gcattcaaaa aagttttcgg
     7501 acctagaaaa gaaaaattcg ttccattaaa cagagaagct ttacaaaaag gtatggatgc
     7561 tacatgttct aaagcaacta actaataaag aaaaagatgt caaaaaaaag acaatagaat
     7621 tatttctatt gtcttttttg ctgataagta ttaaaatatt tttatgactg aaattaagcc
     7681 tataatgata ttttatgaac tataaaaata taatacaaat tacaagcttt tcaattatga
     7741 aaggagaatc tttagtgaga aagtattttg gaacagatgg agttagagga gtagcaaata
     7801 cagaactaac atgtgattta gcatataaac ttggtagagc tggtggattt gtcttagctc
     7861 aaggagacca cagagtaaaa gtagtagtag gtaaggatac tagaatatca ggtgatatgt
     7921 tggaagcatc tttaatagca ggacttatgt ctgttggatg tgatgttatt actgttggaa
     7981 taataccaac acctgcagtg gcatatttaa ctagaaaata tggtgctgat tgtggtgtgg
     8041 ttatatctgc atctcacaat cctgttgaat ataatggaat caaattcttt aataagaatg
     8101 gatataaatt agatgatgaa attgaattga aaatagaaga atatatagac gatatagaca
     8161 aaattgattg tcttccaatt ggtgaaaatg taggaagaaa attacatgaa cattgtgcac
     8221 aaagagacta tgtagactac ctaaaatcaa taataagcac tgactttaag ggattaaaag
     8281 tagttttgga ctgtgctaat ggtgcgtcat ataaagtggc accaatagtg tttgatgaac
     8341 taggagcaag tgtaatttca ataaatagtt caccagatgg aaataatata aactataaat
     8401 gtggctctac acatccagaa caacttcaaa gggctgtttt ggaacataat gctgacttag
     8461 gtcttgcata tgatggagat gctgatagat taattgcagt aaatgaaaag ggtcaaattg
     8521 ttgatggaga ccatataatg atattgagtg ccttaaattt aaagaaaaat aataaattgg
     8581 cacaagatac tttggtagtt acagttatga gtaatatagg gttgacaata gctgctaagg
     8641 aaaatggaat aaatttatct actacagcag ttggagacag atatgtttta gaagatatgg
     8701 ttaaaaatgg atataatctt ggtggagagc aatctggaca tatgatattt ttagattaca
     8761 acactactgg agatggagtg cttagttctc taatacttgc aaatataatc ttgcaagaaa
     8821 agaaaccttt atcagaaatt gcatcaataa tgagccaata cccacaagta ttagtaaatg
     8881 caacaataaa aaatgaaaat aagaacaaat atatggaata tccagaaata aaaactgaga
     8941 tagaaagaat agaatctatt ttagacggaa atggtagagt tttgataaga ccatcaggaa
     9001 ctgagccatt agttagagtt atgttagagg gtaaagagga aggtcaaata aaagaattag
     9061 caacaaactt agctaatctt atacaagaaa aattatcgta atttaaaggg tattctcaga
     9121 tataaatact gaggacacct ttctaagcgc cagaacttag ttatagacat aattattgag
     9181 taatatagga actaagttga cgaggtcaga gttaatcgaa ttgtcggcgg atgctctgcg
     9241 gtgtgtttac catcgaaaga cttctttaaa taataggagc aatcctatgg acaaagggaa
     9301 gccaaagtaa acttatatga cctaactctt aaaatatata aataatattt ttaggaggac
     9361 acaaataatg tgtggaatag ttggatattt aggaagtaga aaggcagcag aagttatagt
     9421 agaagggctt tctaagctag agtacagagg ttatgactca gcaggagtag cagttaatag
     9481 ttctaatgaa aaagaactta atattagaaa gtttaaaggt agactttctg tacttgcaga
     9541 agacttagaa aagaatccta ttgatggaaa tttaggtata gggcatacga gatgggcaac
     9601 tcatggagaa ccatctgatg taaactcaca tcctcatttt aatcaagcca aaacaatagc
     9661 agttgttcat aatgggataa tagaaaacta tatggaaata aaagaagaat taatttctga
     9721 aggtgttaag tttgaatctc aaacagatac agaggtaata gcacatttag tagataaata
     9781 ctatgagggt aatttattag atgctgtata caaaactata tcaaaattaa gaggagctta
     9841 tgcattaggt gttatatgta aagaacatgg aaatgaatta gttgcagtaa gaaaagatag
     9901 tcctcttgta gttggtgttg gtgaaggtga aaactttata gcatcagata tacctgcatt
     9961 attaaaatat actagagatg tttatttctt agaaaatgga gaagtagttc atctaaaaga
    10021 cgaaaatgta acagtttatg actctaatag aaatcttgtt gaaaaagaag tatttcatgt
    10081 aacttgggat gtagaagctg cgtctaaagg tggatatgac tactttatgt caaaagaaat
    10141 tcatgagcaa ccaacaggag taagagaaac tttagaaaga agacttgatg ataatggaaa
    10201 tataattcta gacagtataa atatttctaa agaagactta gaaaaaataa ataaagttta
    10261 tatagtggct tgtggaacag catataatgc tggattactt ggtaagtatg ccatagaaaa
    10321 atttgtaaat ataccagtaa taactgatat agcatcagaa tttagatata gcgacccatt
    10381 tgtagatgaa aattcattag taatattagt tagtcaatct ggtgagacag cagatactct
    10441 agcagtatta agagattcaa aggctaaagg agcaagaata ctttcaataa ctaacgttgt
    10501 aggttcatct atagcaagag aatcagatga tgtattctat acttgggcag gacctgaagt
    10561 agcagttgca tcaactaagg catatactac tcaaataact tctttataca tgatagcttt
    10621 agactttgct atcaaaaaag gaacaataac tagagaattt tatgatagta tgatttctaa
    10681 gatgaaagaa ataccatcaa aaatacaaga aatactagac aatgaagaat atataaaaga
    10741 agttgctaaa acagttgtta gttctgaaca tgcattctat ttaggaagag gtatagacta
    10801 tagcttagca atggaaggtt cattgaaatt aaaagagatt tcatatatac atgcagaagc
    10861 ttttgcagca ggagagctaa agcatggaac aatagcttta atagaaaaag gaactcctgt
    10921 aattgccata gctactcaag aaaagttatt tgaaaaaatg gtttctaata tggaagaagt
    10981 aagagcaaga ggagcttatg tagtagcaat agctcaaagc cataataagg atgttgagaa
    11041 agctgctgat aaaataatct atataccaaa ttcagatgat attttatcac caatattagc
    11101 agttgtacca atgcaattac ttgcttatca tgtctctgta ctaagaggat gcgatgtaga
    11161 taagccaaga aatctagcta aatcagtaac agttgaataa aaacagatta aaaattaaat
    11221 agataatatt tattgaaaag aactcatcct gttatttgta ttttatagga tgagtttttt
    11281 agataaatat taaatttgtt ttgtttttgc tataataaat tttataaaaa gattgatatt
    11341 aaatcatctg taaaatgata aatatataga ccataccagg tatagggaac gataaatata
    11401 gttcatataa aatatagtga gtacttaata aacatatagt caatatcaga taaaaaaaat
    11461 aatagcttat gagaaaaatt aaaatacaag gtattagtaa ccttgtgata agataaagtg
    11521 agtagtttaa aataagaata taaggaacta tttataaaag aatataatga agctaataga
    11581 gtataagaaa attactttat tttagtaaaa taatttcaaa taaaaatttt tttaaattgg
    11641 gagtataaat tatgacaaat gattttaaaa aagaaaagaa agaagattat tttgttaatc
    11701 ttaaggcaat atctactgaa gttttacaag aaaaagtgca agataacgct tgggttatag
    11761 ttgatactcg cttaaatgat gcttataatg gctggaagtt agatggagta aaaagaggtg
    11821 gacatataaa aggagcagta gatttttctg ctaattggtt aagtgtatac tcagatagaa
    11881 aagatgaagt gttagaacaa gcattgaaaa ctaaaagaat tgatttagat aaaaatatag
    11941 tattgtatga tgcaaatgga aaagatgctt tggttgtagc aaattattta tctaaaaaag
    12001 gatataaata tctatataaa tatgatatta aacaatgggc agacgacgaa aatcttccaa
    12061 tggaaagata taaaaattat caaatgatag ttcctgcttt tataataaaa gatatattag
    12121 atggaaaaat tcctgagaca tttgaagatt caaagaatat aaaaatgatt gaagcgagtt
    12181 ggggaaaaga atcgtataca aaagggcata tacctacaag tgttcatgta aatactgata
    12241 ttatagaacc tcctccaaca tggatgcttg ataatgatga taacttaact aagtttgctt
    12301 tagactatgg attaacaaag gatgatactg taatagtttc aagtagtaca cctatggcat
    12361 cttatagact agctgttata ttgagatata taggagtaaa agatgtaaga gttttgaatg
    12421 gtggaacaaa ctcatggtta tcagcaggat atgaattaga atttattagt aatccaaaac
    12481 attcatgtac taattttgga gcagatatac cagttaactc acaattaatt gttactacat
    12541 ctgaattaag acaaaaacta aaagaaaaaa ataagtttat actagtagat aaccgtactt
    12601 gggatgaaca tataggaaaa gtatctggat atacatatta tgataaaaaa gggcgtatac
    12661 caggagcttt atatggacat tcaggtagtg atagtgtttc actagaagaa tatcgtaata
    12721 ttgataatac aatgagaaat aaatatgaga ttttagaaat gtgggacaag gaaaatatag
    12781 atgtgaataa acagcttata tttatgtgtg gaagtggatg gcgtgctgct gaagtattaa
    12841 catatgcaaa tgtcatagga gtggaaaata cctcccttta tagtgatgga tggatgggct
    12901 ggagtcttga taattcaaat ttaattgaag tgggagaaca caaataaagt ttttgaaagt
    12961 gcaatagagt tgtttaaaac aaaggtgttt ttgatttatt aggaagagtg tgtaaattgt
    13021 gtattgggaa gtgtacttag gtatgttcat ttggagctat ggaaattgac aaaaaagtat
    13081 tatctgttta ttttgttggt aaaatatgaa tagattatag atttaagtat agactaagta
    13141 atttatatta cagaagagaa tatataagaa ttaatgcaag atgtatttga tacatataag
    13201 aaattggcaa gtccaggaga aggatagaga aaattgagat tgaagagttt gaaagagatt
    13261 attagatgaa aaatacaaaa atgtataaaa atacatattg agaaagtata tctctcttaa
    13321 atttgttaat aattctatta tacagaaaaa catatttaag ggggattttt tactatgaaa
    13381 aaagtaaaat tagctataag cttttttata ttaatgatta taggaatgac aatatcttat
    13441 gcagatacaa gtataaatac tagatacaat agattaagta aagctcttga aatatcagaa
    13501 gcaaatttta aattttataa tgttaaaatt aattctgtta taaatcataa tttatcaaat
    13561 aaagaatttc ataatatttt tatagaaata attaacaatc taaagttgga taaaaaagga
    13621 ataaattgga atcaaaaagg gtctggcgat aaaatacaat tatgtgctac tataaaaggt
    13681 agcgataaag aaatttctat aaaaggaata aataaaaata acaaagaaag ttacatagta
    13741 gttgacatat tggataacaa agtatataaa cataaagagg ttatttatag taacgtagaa
    13801 aatgtgttaa ataaatattc atctcaagta gatgtgtctg cttgtattgt tggagaatac
    13861 acaaaaaggt tacaaaacaa caaatatgat gatattttac aaaaaatatt atataatatg
    13921 agtgctaagc aaatagatga agttaaagac gtaaactttt tatctataac agcatatagt
    13981 aaagtattaa atgattatga attagaatat ttaggggaaa aaattaattt aaatatagga
    14041 attaaatata atgaggatga cggaaaaaca ctcatatata ttgccacacc aattataaaa
    14101 ttagattatt aattatgagg agatcaaaaa atatggctaa gataatagta aaaaagagta
    14161 atccacttaa aggtagtgtt aaaatagatg gtgcaaaaaa tgcagtttta ccaataatag
    14221 cagcgacttt attagcaaac ggaaaatcaa cattaaatgg agtaccaaat ttaagagatg
    14281 ttcatgttat atcagattta ttaagacatg taggagcaga agttgaatat aaagaaaata
    14341 cacttactgt tgatgcaagc aatataaaaa cttgtgaagc tccatatgag cttgtaagaa
    14401 agatgagagc ttcttttttg gttatgggac cattgcttgc tagatttaat agtacgaaaa
    14461 tttctatgcc aggaggatgt gctataggaa caagacctat agatttacat ttaaaaggat
    14521 ttaaagcctt aggtgcaaag atagaaatgg accatggttt tgttgaagca gcaacagaaa
    14581 agttagtagg aaataaatta tatttagatt tcccatctgt tggagcaact gaaaacataa
    14641 tgatggcagc ttctttagca gaagggacta caataataga gaatgcagca gaagagccag
    14701 aaatagtaga tttagcaaac ttcttgaacg aaatgggagc agatgttaaa ggtgcaggta
    14761 ctaatactat aaaaataaaa ggtgttaaag agttaaaagg agcagaacat aatgtaattc
    14821 cagacagaat agaagcagca acttatatgg ttgcagcagc tatgacaaaa ggtgatataa
    14881 ctgttgaaaa tgttttaatg gagcatttaa aaccagttgt agctaaatta agagaagctg
    14941 gttgtgaaat aacagaaatg gacaattctg taagagttgt aggtccaaaa gtattaaaac
    15001 caatagatat aaaaacttta ccacatccag gatttcctac agatgttcaa gcacaattta
    15061 tggctatgct tactgtagcc aatggaactg gtgtagttat agaaactgtt tttgaaaata
    15121 gatttatgca tgttgctgaa tttaatagaa tgggtgctaa tataaaaata gatggaagaa
    15181 gtgcggttgt taatggtgta gatgagctac acggtgcagc agtaaacgct actgatttaa
    15241 gagcaggggc agcccttatt ttatgcggtc ttatagcaga aggagaaact caaataggag
    15301 aaatatacca tatccaaaga ggttatgttg atatagataa gaagataaca gcactaggtg
    15361 gtcaaatcga aatagtagaa gattaataat tggtttatag aagaaagtta taaattaata
    15421 gggttgttca tatacttaaa tacaaggttt taaaagctaa ggaggagagt atatgaagaa
    15481 cccattggtt gttttgttag gatttgtgac ttgctctgtg ttagttccat cacttataac
    15541 tttagtttct tataaaaatg tagaattaac tgaaaaacca gagagcccag tatctataaa
    15601 caaaactatt aaaaaaagtg atattgaaga caaaggtaat aaagaagaaa aaaatacagt
    15661 gaactatgaa actgtaaata aaaaagcacc aattatcaat gtatataatc atataacagg
    15721 gaaaaccgaa aagatggaca tggagaacta tttatgtgga gtactagctg gtgagatgtc
    15781 ttcagagttt gatatagaag ctttaaaagc tcaatctgta gcagctagaa catatgtagt
    15841 atataaacag gaacatggta aatctagtaa acataaaaat gcagtagtat gtactgatta
    15901 taaacattgt caagaatata agagttatga tactcttaaa aaattaaatg gtgaagaatg
    15961 gattaagaat aaatattcca aaatccaaga agcagttaga ggaactaaag gtcaaataat
    16021 aacttataat gataaggcga tacttcctct ttatttttct acatcttcag gaaaaacaga
    16081 aaatagtgaa gaagtatttt ctgcaaaata tccttattta aagtctgtgg aaagtcctta
    16141 tgataagtat tcccctaaat ttgcgtcaac acttaaaata agtaacacag attttgttaa
    16201 aagtcttaga agggcatata gcactattgt gatagatgtg aataatttaa gtaaacaagt
    16261 ttcaataact aaaagaagtg atgccggaac tgtagaaaaa ataaaattag gaaataaaga
    16321 attgactgga aaagacatta gaactgtttt taaattgaac tctgcaaact ttgacataaa
    16381 atttggagaa ggatacatag atttcgtggt aaaaggatat ggtcatggtg ttggtatgag
    16441 ccagtgggga gcagaaggaa tggcagaaga gggctataaa tattatgata tattatcaca
    16501 ttactacaca gatacaaaaa taaaagatat atactaataa ttgtataaaa tgccaaaaac
    16561 acgtcaataa tccaaatgta gaaatatatt tggaggtgca aaatgaagaa aaagctgtta
    16621 gaaaaagatg gtttttattt atctttgttt gtatgtgttt gtttattagc agtaggtgga
    16681 gtttggttta caaataataa tgtagataaa ttggcttcta ataaaggaat tatggaaaat
    16741 gctaataaag atagtgaaga ggaaatacat ttaattgaaa aagacaagaa agatgctatc
    16801 cctactgcaa cggattcaaa acaaaactta gaaaaagcta agtcaaaaga ggaaaataaa
    16861 tcgagtacaa caaaattaaa ttacattgga gataaagtaa taagaggata ttcagaaaaa
    16921 gagcctagtt attctaaaac acttgatgtt tgggaaactc ataaaggtgt agatattagt
    16981 tgtactaaag gcaaagaagt aaaatctcta ttaaacggaa tagtagtaga tgtatttgat
    17041 gatgaagaat atggacaatc agtcaaaata aaaagtgaca ataacattgt agtagtatat
    17101 tcaaatctag ataaaaatgt tagtgttaag aaagaacaga aggtaacaga aggacaatct
    17161 ctaggtactg taggtagtac ttctcaaata gaaagtgaag aaggtataca tgtgcattta
    17221 gaggcgtata gtggagaaaa atctatagac ccaatgagtc taattaagta atagtataac
    17281 aagtttaatc atatttggtt aagcttgtta tattttttgg tgtttttaca tatatatcat
    17341 taaagctatt atgtgagggg ggtaatcttt gagatctcat atagaggaaa gggccatagt
    17401 ggtagcaaaa tatatacttg aaaaaaacac tacagtaaga caaaccgcca agacatttgg
    17461 agtaagtaaa agtacaattc ataaggatgt tacagaaaga ttgaaggaaa taaatccatc
    17521 ccttgccaaa gaagttaaaa atgtgctgga caaaaataaa tcagagagac atattagagg
    17581 gggattagca acgaaattaa aatatgagaa ggaacataaa aaaatgtagg ttaaaaacct
    17641 acttttttta ttgaattaaa ttataaaaag aaatatatta agtaattata aatagcaaaa
    17701 taataatact ctcaaaagat ttattttctc ttgattaaag cgagattttt gtatatgctt
    17761 tattatagga gtattaaaat aggcttataa tataatctaa tattataaat aaaggagttg
    17821 aaaaaatggc tggagctgat ataggcatag atttaggtac tgcaaatgtg cttgtttatg
    17881 ttagtggtaa gggaatcgtt ttagaagaac catctgttgt ggcgatagac aaaagaacag
    17941 attctgtttt agctgtggga gaagaagcta aaaagatgat agggagaaca ccaggtaata
    18001 tagtcgctat aagaccttta agagatggtg taatttcaga ttatgatgta actgagaaga
    18061 tgttaaaatc ttttatagat aagatagttg ataaaaaggg atttggaaga ttttttatgc
    18121 caagaattat ggtatgtgta ccaacaggtg tcacagaagt ggaaaaaaga gcagtagaag
    18181 atgctacgag acaagctgga gctagagaag tatatatcat agaagaacca atagcagctg
    18241 ccataggagc tggtgtagac atatcacagc ctaatggtaa tatgattata gatattggtg
    18301 gaggtactgt agatatagct gtaatatctc taggaggagc tgttgtaagt gaatctataa
    18361 aggttggtgg agatagattt gatgatgcta tagttaaata tatgaaaaaa caacacaatc
    18421 ttcttatagg agaaagaaca gcagagaaaa tcaagtttga aataggttct gcatttaaga
    18481 gagaagaaga aaaatatatg aagataacag gaagaaattt aattacagga cttcctagtt
    18541 ctataactat aaattcaaca gaaatgttgg aagcacttag agaatgtgtt gagcaaatag
    18601 tagttgctac tcatgctgtt ttggaaaaaa cgcctcctga gttagcagca gatataggag
    18661 atgtcggtat aataatgact ggaggaggtt cattattata tggattagat aaaataatag
    18721 aacagaggac aggaataact gttacaatag cagatgaacc attatcttgt gttgcaaaag
    18781 gtacaggaat agctttaggt tcaatagact tacttgaaac tggtgggtct ttcaaaagaa
    18841 aataattagg agagtttaga atgttaaata tagatgaaat aaaaaaatta ataccacata
    18901 gatatccatt tttattagta gacaagatta cagagctaga agttgggaaa agagctgttg
    18961 gtataaagaa tgtaacagtt aatgagccat ttttccaagg tcactttcca gaatatcctt
    19021 tgatgccagg agtactaata gtagaagctc tagcacaagt atgtggagtg gcaatgatgt
    19081 ctgtagagga aaacaaagga aaactaggtg tatttgcagg tatagataaa gttagaatca
    19141 agagagaagt aagaccagga gatacattaa ctatggaagt tgaaatgaca actcttagaa
    19201 aaaatatagc taaggcagat gctaaggcat atgtaggtga agaacttgtc tgtaaaggtg
    19261 aattaatgtt tgctttagta gaaaaataaa aacttgaaat ataaaagtaa aaaggtagat
    19321 aatttggagt atttaatcag atactctaaa atatctacct ttttatataa agaattttat
    19381 catttagttt atatttagat taggtggata ctaataaagc caaggctatt gtttcagcta
    19441 aatctaatat aaagctaagg cgtatattat taaatgaaag tttattattt tcatcaattt
    19501 tatctacaat tcctactata gaataatttc ctatttgagg tagtttttta ccaacacctt
    19561 ttccgggcag gataggtcct tctctaatct gtatattacc tatgttactc tgcgacccca
    19621 agcaagcatc tatagccaag aagttacctt gtatatgtgt attttttata gtatctaaag
    19681 actcatatat atttaaggcg tgaatagggt tatctagtgt accgtaaacg gggtatttaa
    19741 agttactgtt cttaagtatt gttccaacta aagggcctaa agtatcacct atagccctgt
    19801 ctgttcctat gcaaacaaca atcgtatttt cattaattat agtttttaga actgaactta
    19861 gcatctgtat aacatcatca tcgctgtagt ttactttagc taaatacaat aggaacaccc
    19921 ccttcaaaaa aatatatgaa taaaaagcat taaaaagacc taactttaaa tttcagatag
    19981 atagaaaatt ttatttgtct tacaaaaaaa ggaatagaaa aaatatgact aaaaatattg
    20041 acaaaatttt aaaaaaatag taagctagta aagtaaaaca aaacttaaat cttgattacc
    20101 ttatcaagag aggctgaggg ataggccctg tgaagcccag caaccaccca aatgggaaaa
    20161 ggtgctaatt cctacaagac aaaagtcttg aaagatgagg tcagttaatt agtatgtaat
    20221 tacgattatt aacctcttct tataaaggag aggttttttt attttgagta aatttctaaa
    20281 attaaaaata aggtaaaacc tataataaaa attaagaata aataaggggg aataaaaatg
    20341 gcaagacatt tatttacgtc agaatcagtt acagaaggac atcctgataa gatatgtgac
    20401 caaatatcag attcaatatt agatgcatta ttagaaaaag acccacaatc tagagtagct
    20461 tgtgaaacta cagttactac tggtttagtt ttagtagcag gagaaataag tacatctgct
    20521 tatgtagaca ttcctaaatt agtaagagaa acagtaagag aaattggata tacaagagca
    20581 aaatatggat ttgattgtga tacttgcgca gttataactt ctattgatga acaatctgga
    20641 gatatagcta tgggagttga tgaaggccta gaaagtaaga ctggagaaga gatagaagaa
    20701 gaaatagaaa aagttggagc tggagaccaa ggtataatgt ttggatttgc atgtaatgag
    20761 actccagaat taatgccact tccgatatct ttggcacata aattatcaag aagacttaca
    20821 gaagttagaa aaacaggttt agttgattat ttaagaccag atggaaaaac tcaagttact
    20881 gttgaatatg aaggaagtaa agctgtaaga gtacatacag ttcttatatc agctcaacat
    20941 tgtgaaacag tatcaaatga taaaataaga gaagatttaa ttaatcatgt tattaaggaa
    21001 gttataccag cagaattact tgatgaagag actaaaattt atataaatcc aacaggaaga
    21061 ttcgttatag gaggaccaca aggagataca ggtcttacag gaagaaaaat aataatagat
    21121 acttatggtg gatattctag acatggtgga ggagctttct caggaaaaga ccctacaaaa
    21181 gttgatagat ctgctgctta cgcagctaga tatgttgcaa aaaatattgt tgcggctggt
    21241 cttgcagaca aatgtgagat agaacttgca tatgctatag gtatagctag accattatct
    21301 atatttatag atacttttgg gacaggcaag gtttcagaag aaaaacttgt tgaattagta
    21361 aataaacact ttgatttaag accaggagcg ataataagag acttagattt aagaaagcct
    21421 ctttataaaa aggttgctgc ttatggtcac tttggtagaa ctgatataga tttaccttgg
    21481 gaaagaactg acaaggttga gcagttaaga aaagatgctt taggtgaata atttttaact
    21541 aaaaagctgt tagaatgtaa ttttacgatt actttctaac agctttttta ttatgagtta
    21601 aatattttta ataaatgtta atttatcatc atataatgaa taaacagtat ttaatctggt
    21661 caatacattt tatataatac tctactaaat ttatacatat tatgggatat atgttaaagg
    21721 atggttaaga atgtgttaac taatgttaaa taattaaaaa tatattgaaa tatcacaata
    21781 atgagtctaa tataatctta ataaacttac actttaaaaa taattttaat tgggggggag
    21841 tagtgtatgt caggcaaagt aaatattaaa acggttataa gttttgctgg agcttatgtt
    21901 gctactgtaa taggttctgg atttgcaact ggtcaagaaa tactgcaatt ttttacgttt
    21961 tatggatatg cgggtattat aggtggaata atctctatgg tactattttc atggtttgga
    22021 gcagaagtta tagataaggg aagagaattg aaacttaagg agccaataaa aatttatcaa
    22081 gtatattgtg gaaaatatct tggaacattt tttgaatggt ttggtccatt gtttttattt
    22141 ggaacattag taataatgat tgcaggagca ggagctacat tatcagagta ctatggatta
    22201 aatccatatg taggtaggat tggtatggca atcgtttccc taataactgt ttcattaggt
    22261 ttgacaaggc tttctaaaat actaggtaat ataggaccta taataataat atttacactt
    22321 ttagtaggag caattagttt atttagcaat attgatgctt tatcaaatgc tggaaatatg
    22381 gttgatagtt taaatattaa aactgctaca tcaaatggat atttctcggg agtactttat
    22441 acttgttaca atgtaataat agtaataaca ttcttgacag gaatgggagc ttctgcagta
    22501 aataagaaag atgctgtatg gggtggtata gtaggtggag tagcacttat ggcagcagct
    22561 ataatgatga atttggcatt actttcagac ataggaaata tatacacaaa agagatacca
    22621 gcactttact tagctgataa gatatctcca ataataggta ttttattctc agtagtactt
    22681 ttactaggaa tatacactac agctgtacca ttgttatggt cagtaacaaa ccgatttgta
    22741 gaagatgacc accctaagtt taaaattata acaatagtgg taagtatatt agcttgtata
    22801 ggaggtttac tgccatttga taagttagta ggtacactat atccatatac aggttacatg
    22861 ggaattttaa ttttactatg tatgctttat agaagaatta ctaaaactga ggggtacaag
    22921 gagaataaat ctgaaataag ttagtactgt aatatactta tgaaatttaa tttatactct
    22981 aaatatagag taattaaaat aaaatagcta tttaaatatg gtacctgtat ttcatggtac
    23041 catattttat ggatgcagat tttttttgtt tctgataaaa tgtataatag ttggaaatta
    23101 cgttaagtat aaaaatataa aacttttcta cgattatcat agttttaata tataaaatta
    23161 tactaacatt taaatggagc gattattagt atttttagga gataaaaatg gaaaaattag
    23221 aaggaatgat aagtgaaata gtctttaaaa acgaagataa tggttataca atagctcacc
    23281 tggtaaatga aaatgatgaa attgtggttg taggatgtat gcctactttg gcaattggag
    23341 aaagcataga agttgaagga aagtgggtaa atcataagat ttatgggact caatttgaag
    23401 tgaatagttt tatgccagta acaccatcat ccttagaagg aatttatgtt tacttatctt
    23461 ctggaatgat acatggtatt ggtgaaaaaa tggcaaaaag aataattgat aagtttggtg
    23521 tagatacgtt agaggtgata caaaattcac cagaaaaact acaagaagta gaaggcatag
    23581 ggagtaaaaa agtaaaacaa atagtaaaaa gttatgaaga agacagagaa ttgagaaata
    23641 taataataca gctatctcct tttggaataa caccaaatta ctgcctaaaa atatataaaa
    23701 aatataaaag cagtgcaata gaagttataa ataaaaaccc ataccaactt gctgaagata
    23761 taagaggaat tggatttaaa gtagctgata gtatagcaag taaaattggt atagataaaa
    23821 actctaaaga tagaatttgt cagggaatct tatacacttt aaataaatct ttaagtaatg
    23881 ggcatacata tttaccagag catgttctaa tacaagattc ggaaaaatta ttggagctaa
    23941 atggagaaat aataaaagag tgtgtaatga tgctagttta taatcaaaaa atacatatag
    24001 agaaagtaaa caatgaaaat cttatatacc ttatgccata ctatttagct gaaaatggag
    24061 tatgtagtca aatagtaaaa ttatcacagt atgaatttga agacttaaaa atagatatag
    24121 ataacgagat taatgtttta gaagaagata agaaaattaa gttagcagaa aaacaaattt
    24181 tagcagtcaa agaatctgtt aatagtggag tattaattat aactggtgga ccaggaactg
    24241 gtaaaacaac taccattaat gctatcatag atatatttga aaataatggt aaaagtgtga
    24301 ctttggcagc tccaacggga agagcagcaa agagaatgag tgaaacttct aataaagaag
    24361 caaaaactat acataggttg ctagaaatgg gattttcaac agatgatgat ttaacttttt
    24421 ttaaggatga agaagaccca ataaattcag atgtaataat tgttgatgag gtatcaatgg
    24481 tagacattat cttaatgtac aatttactta gagctataaa gttaggaaca agagtaattt
    24541 tagttggaga tagtgaccaa ttaccttctg taggtgctgg aaatgtactt aaggatatga
    24601 ttaattctaa tattataaat gtagttaaat taaatgaaat atttagacaa gcgcaagaaa
    24661 gtatgataat tgtaaatgcc cataagataa acaatggaga accactttat ctaaatacaa
    24721 aaggtaaaga tttctttttt ataagaaaat caactaatga agaaatttta aatgaaataa
    24781 taggattggt taatgaaaga ttacctaaat tttataaagt tgataaatta aaagatatac
    24841 aagttttatc ttcaatgaga aaaggtgaat tgggagttac taatctcaat atagagttac
    24901 agaagtatct taacaaaaaa gaaaaattta aagttgaaga aagtttttct aaaagattat
    24961 ttagagttgg cgataaggta atgcaagtaa aaaataatta tacaaaaaaa tgggaaacag
    25021 aagaccaaaa agaaagtgga gaaggtatct ataatggaga tataggatat gtttatcaca
    25081 tagataaaga taagaaaact atttatatat tatttgacca gactaaaatt gtatcatacc
    25141 tatatgatga attagatgaa attgaccata gtttttgtac aacaatacat aaaagccagg
    25201 gaagtgaatt tccagttgta gtattgccaa tagcgtgggc tcctcctatg ttacttagcc
    25261 gtaatttatt atatacagca gtaacaagag caaaaaaatt agttgtatta gttggagatg
    25321 tgaaatatct tgaatatatg ataaaaaata atagagttaa tcaaaggtat tcaaatttag
    25381 gatataagct aaataaattt aaacaagaag ggttgttgat agaataaata tgaaaatatg
    25441 caatgataat tttattaaga attttaaaaa tatgattaat aaatgtttgg attttatata
    25501 tcctgagaat ataagttgca taatttgtga taaatccata aagaaaacaa atacttattc
    25561 aatatgtaaa agttgtttta aagaaatgaa ttttatacaa gatggttgca tgaagtgtgg
    25621 aaaaccaata atatgtcatt caattgaaaa agaatttatt gaagagtgta gttattgttt
    25681 taataaagac ttttattttg ataaatcaat atcttgtatt gaatacaatg atgtgacaaa
    25741 aaagatggtg ttagggctga agtataacca aaaaaccttt atggctaagt atatagctca
    25801 aataatgaaa gaaaaattat atttagaaaa cataaaattt gattatatat tatttgttcc
    25861 acttcataaa aaaaggctaa ataaaagagg ttttaatcag gcacaaaaaa tagcatttaa
    25921 tttaagtaaa atagtaaata ttccattatt agattgcata agtagaaaaa aatatacaag
    25981 aatgctatat aaattgaata agaaagaacg aaaagaagaa ttaaaaaatg tatttgttgt
    26041 taaagaaaat gttaaattaa taaataataa aaatatactt ttaatagatg atatatttac
    26101 tactggtcta acaacaaacg aaatttctaa acttttaaag ttatcaggtg ctaataaggt
    26161 ttttgtattg actctactta caaaagcaaa tgataattat attatggaat aatcttatat
    26221 attgacaatt ttagtttaaa atattaaaat gaataagtga tatttaggtc tgtggttgaa
    26281 agtccaagcc agtcgcaggc aaagggatcc acgtaagtta acactaaaaa ttgggttaat
    26341 gatcatggtg cggcttagaa gtaagaccta ccgataataa aatcgagaga gagtagtagt
    26401 gcggcgaacc caagcgaacc ctccagtagg cgagtgtggg gtcaaaaacc aggtcagcta
    26461 aatatcatat taaaagagag ctagcatacc tttatgtata gctctttttt atatataata
    26521 aatttaggtt gagtcataaa gaggtgatat gtttgaataa aagacaagaa aatttattaa
    26581 caacattgct attaaaagaa gagtttataa atgttaataa gattgccaat gattttgagt
    26641 gttcagaaag gacaataaga aatgattgca aatatattga tgaatggtta tctacttttt
    26701 gtaatgctta tatcaaaaga aaacctaata ttgggattaa atttagtgga gatggaacag
    26761 acaaaatact ggtaaatcag aagttgcgag gtgaagttaa aagagtaaat agtgagcttc
    26821 ataagcaagt tgagatatta aatttactac tttgtaataa taaaaaagtt actttaaatg
    26881 atttatctaa taagttgtac ataaataaaa atattgtcag agaagaaata agtaaattat
    26941 cagtagcatt aaataattat aatttaaata tctcgactaa aaaaggcagt ggtatatata
    27001 ttgaagggga tgagaaagac ataagaataa tgttagtatc attcttattt aaatatatag
    27061 ataaatacag attaaacata gatgaaatag agtacttcca tatagtagat ataataattg
    27121 taaaaaacat aatgcttact atagaggagg atatggatat aagacttaca gatatatcat
    27181 tcaagcaatt gatgctattt ttgcttataa atataatacg aataaggctg ggaaattctt
    27241 taaaagtatc aaatactaag aaagagctta agctaaaaat gcttatattg gatatagaag
    27301 atgaattgaa atctaattta tccatagtat taaattatga agaaaaattg tttatagaat
    27361 ctttaatatt gggtatgaac aaacaaataa atagtaataa tattaagaaa tctttatatc
    27421 taaatgaaga attagcaagt tatacaaaaa aaattataaa tttagtatca gaagaatcag
    27481 gaataaattt taataatgat atattacttt atgaacaatt aatatgtcat ttgaatgtta
    27541 caatgcatca aatgaaaagc aatgtttact tagaaaatcc acttttagat aacataaaaa
    27601 ctaaattttg ttttttattt acagtaatat caagttcaat agaaattgaa ttaaaaggaa
    27661 aacaaataac ggaagatgaa attggttatc ttacacttca ctttcaaacc tctctagaaa
    27721 gaaaatacag caaaagggaa tcaaataaaa aggcaagtat tgtatgtcca tttagttttg
    27781 gagtaagtat gctattaaaa gtaaagatag aaaagagatt tgataacata aaaattattg
    27841 aaacattaag agaggaagat ttaaaaagag ataactttaa taagactata gattttataa
    27901 ttgcatttca agagtatgaa aatatccaaa aaccaatttt tataacaaca ccattattta
    27961 ctgatgagga tgagaataaa cttaaagagt tcacaaacaa aataaaagaa aaagacactt
    28021 catataaact tatgaacaga ttaatgatgt ctgattattt aataagagag ttagaacatg
    28081 atgatattta tgaagctata acatatttaa ctaatttatt aattgaaaaa cattatgcag
    28141 aaaaagagta tttacaatct attataacaa gagaaaagtc atatccaacc aatgttggaa
    28201 aaggaatatt attccctcat ggagacatga aatatataaa acaatctgtt ttatgttttg
    28261 caaggttaaa aacaccaata aaaattagaa atggaaagga tataaagatt atacttttgt
    28321 tagcatataa aaaagatgat gatagaaaag tatttagaga attatttaaa gaaatatcta
    28381 atttaacaga ggatgaaaat atgctagata ttctaagtaa atgtgacatt gaaaaggtaa
    28441 aagatattct tatataaaat tcatagtgaa ctatggataa gcacaaaatt ttccatgtga
    28501 tggaaaaagt tgtgcatgtt tttttgtttt tataagatat tatatgtttg taaggtataa
    28561 ataatctaaa agtagagaag caacttagga ggaaaaaatg gaagaaatag ttttaaagat
    28621 tataattcat gctggaaatg caaaatctat gctttatgag gcacttgact atgcaaagga
    28681 aaacgatttt aaaaaagcag atgaattaat tgaaaatgca aatgaagaaa ttttaaaagc
    28741 acataaagta caaactgaac ttatacaaaa ggaagctggg ggagataaat cagatatatc
    28801 tattttattg attcactctc aagaccattt aatgacttgt atgagtgaga gaaaccttat
    28861 aaaagaaatg attatgttaa gaaaagaaat tcaaaaacta aaataaaaaa ttaaggggga
    28921 tttaaaaaat gaaaagaaaa gtatatttat tttgtagctt tggtatgtca acaagtttac
    28981 ttgcagataa aatgcagaag gtagcagatg aacacaattt accaatagaa gtagaggcat
    29041 ttccgatagc agaaattgac aaaatagttg aagaaaaaaa tccagattgt atattattag
    29101 gaccacaagt taaatacatg ctaaaggagt taaaaccaaa gtttgaagct caaggaaaac
    29161 ttatagatat aatcaatgaa gttgattatg gaactatgaa tggggaaaaa gtattaaaac
    29221 ttgctataaa gctaataaaa tctaaacaag cgtagaatta tagtaaaaaa taataggaaa
    29281 tgagggtgga tagatgaata gattggaaag aatacttatg ccattggctg aaaaaatagg
    29341 taaaaataaa ttattaattg caattagaga tggtttttta gtatcatcac cattattaat
    29401 aataggttca ttatttttat tagtagcaaa ttttcctata aaaggatgga atgaatttgt
    29461 aggtcaaatt tttggtccag attgggctgt aaaattgcaa caaccaactg tagcaacatt
    29521 tgaagtaatg actctacttg cagttttagg gattggatat tcttatgcaa agcaaataga
    29581 tgtagaccca atagcatcag cagcagttgc aatagttgcc ttttttataa taacaccatt
    29641 tgtaatacca tatacaccag aaggaacaga atcagtttac atggtaacag gtataccact
    29701 aggttggatg ggttctaagg gaatgtttgt aggtatgata acagcaataa cctctgtaaa
    29761 attatttgct gcagttgtaa aaaaaggatg gacattgaaa atgccagatg gagttcctcc
    29821 aactgtatca aaatcatttg cagcattaat accaagtgca atagttatgg tagttttctt
    29881 tttagttaag atagcttttg aagctacacc atatggaagt atacatgaat ttatatttaa
    29941 gtttttacaa atgccattat taaaattagg aaatagttta ggtgctatgg caatcgctta
    30001 tatattctta catttattct ggttctttgg aataaatgga agtagtgttg ttggggctgt
    30061 gtataatcca atattaaaaa ttctatcagc tgaaaactta gctgcttttc aagctggttc
    30121 aaaaatacca aacataataa caggtcaatt ccaagatatg tttgcgacat ttggtggagc
    30181 aggtagtaca ctatcattag tcatagctat gttattaata tgtaaaagta aacgtataaa
    30241 gtcaatggga aaactatcaa ttttaccagg aatatttggt ataaatgagc cattgatatt
    30301 tggtctacca ataatgctta acccaatact attgatacca tttgcaatag ttccaacaat
    30361 aaatataata atagcttact tctgtatgtc agcaggatta gtgcctctta caaatggagt
    30421 tcagttaccg tggacaactc caattatatt ctctggtttt ttaacaacag gatggcaagg
    30481 tgcagtctta caattagtac ttcttatact aggaatattt atgtatatac catttataaa
    30541 aatgctagat aagcaatact tgagagaaga aaatgaaact gttgaagagg aagatgatga
    30601 tatatctttt gatgatttag tattatagga ggaagattat gaagaaaata gttatgatat
    30661 tggaccaaat acaagctgga gctggaggaa aagagaaaag taatatacct cctgctggaa
    30721 aatcaagccc attaggacca ggtgtaatga tggagcaatt tttaaatgaa tctaaggtaa
    30781 tagctacctt attttgtggg gatgaatttt ttgtaaataa tcaagaagaa gtaacgagta
    30841 agatgatagc tatggtaaaa aaattaaacc ctgatgtagt aatctgtgga ccaagcttta
    30901 attacgaaaa cttttctaag atgtcagcaa tactgtctaa aaatataaat gataaaacag
    30961 atataccagc ttttgcagct atgtcagaag aaaatataga tgtgataaac gaatataaaa
    31021 atgacatatg tatagttaag actccaaaaa aaggtggaat tggtcttaat gattcattaa
    31081 ataacatatg taaattggca aaagcaatag caaataaaga agatataact ttgatgaaag
    31141 aagagttttg ttatcaataa gagtattaat tattagcaag tttaaatatt atactattta
    31201 taataagcat acattagtaa aatctatata atttaaggag gggaatttta acatgtgggg
    31261 aatgatagcg acatggagaa tggccctaga aggaataact gaggcatcag aacatttaga
    31321 aaaaggcatg gaagcaggtg atgctgtaga gcttgctgta agaagagttg aagactatcc
    31381 attctataaa tcggttggat atggtggact tccaaatgaa aattgtgagg tagaacttga
    31441 cgctgcattt atggatggta acacattaga tattggcgca gttgctggta taagagatta
    31501 ttctaatcct gtcagcatag ctaggaagtt aagttatgag aaggtaaata attttttagt
    31561 tggaataggt gctgaagatt atgctcataa aaatggattt gaaagaaaaa atatgttgac
    31621 tgatagagcg aagttacatt ataaaaagag aaaaaaagag actttagaca aaggattaag
    31681 cccatatgct ggtcatgata ctgttggtat gatatcttta gacaaaactg gaaaaatgtg
    31741 tgcagctaca tcaacaagtg gattgtttat gaagaaaaga ggtagaatag gagattctgc
    31801 actatctggt tcaggattct atgttgatag tagtatagga ggagctacag ctactggttt
    31861 gggagaagat ttaatgaagg gatgtatatc ttatgagata gttagactta tgaaagaagg
    31921 gaagcatcca caagaagcat gtgatattgc agtaaatgaa cttgataaac agcttatcga
    31981 aagaagagga aaagctggtg atttatcatt agttgccatg aataataaag gtgaatgggg
    32041 agtagctaca aatatagctg attttacttt ttcagtagta actaaagaag aatctcctac
    32101 tgtatatatt tcaaatagag tagatggaaa gactatacat aaggttatat caaatgagtg
    32161 gatggaagca tatgaggcta gaataaaagc accaatagaa gtaagtgagt aaagtagtgg
    32221 ttacataatt aaaatagagt tttataataa ggagattttt atgaatatca taagtaataa
    32281 aatagatgag ttaaaagaag atttattaag tgatattata gatattgtaa aaatacctag
    32341 tgtcaaaggg gagtcagaaa atggcttccc ttttggcgaa aaagtagggg aggcacttaa
    32401 taaagcatta gaaatatcag aaaaattggg atttaaggtt agaaacttgg ataattatat
    32461 tggttatgca gagcatggag atagtgatga ttatgtttgt gtaattggac atgtagatgt
    32521 tgtacatgaa ggagatggtt ggaagcatca gccttataag ggagaagaaa ctaatggaag
    32581 aatttatgga cgaggagtac tagacaataa aggacctata atgtctgcct tatatggatt
    32641 atatgctatt aaagaattaa acttaaaatt agataagaga gttagaataa tatttggtac
    32701 aaatgaagaa agtggatttg aagatatacc atattattta gaaaaagaaa aagcacctat
    32761 tatgggattt acaccagatt gtaaatatcc tgttgtatat ggtgaaaaag gcatggcaaa
    32821 gattagaata aaaagtaaaa tcaattatga agaagatgta tacctaggtt ttatagaaaa
    32881 tatgtctgaa aatgtgcttg ttacatataa agagctgaat atagaaaata gtgatactat
    32941 attagatata aaggtaaaat acgattttag ttataaattg aaagatgtat tggatgaaat
    33001 aaaagctagt tttccaaata gtatcgatat agaggtaatt tctaatttta atcctgtata
    33061 ttttgataaa gagagtaatc ttgttaaaaa gcttcagcta gcttatgaaa gggttacatc
    33121 attagatgga actcccgtaa caactaatgg aggtacgtat gcaaaagtaa tgccaaatat
    33181 agttccgttt ggaccttcat ttccaggaca aaaaggaata gctcataatc ctgatgaata
    33241 catggatata gaagatataa tattaaatgc aaagatattt gcaaatgcaa tatatgaatt
    33301 agcaaaggag tagttatgtt agaaataata ggaatgtcag tagaagacgc taaaattata
    33361 gaagattgtg gtgcagatag aatagagtta gttagtgctt taactgaagg tggattgaca
    33421 ccaagttttg gtcttataga aagtgttgtt aacagtgtaa agatacctgt gaacgtaatg
    33481 ataagacatc atgcgaaaag ttttgtatac agtaaagaag atataagtat aatgcaaaaa
    33541 gatataggtg tggttaaaga gattggtgct aatggagttg tatttggtgt attggataaa
    33601 aacaataata tagatgagaa aaatctaaat gttttattga aatgttgtga taatcttgat
    33661 gttacatttc atagagccat agatgaatca aatacaatag atagtgttaa aattttgaag
    33721 gattatgata aaattacgaa tatattaaca tcaggaggga aaggaagtat agttgataac
    33781 atacaaatga taaagaatat gatgcttagt tctaatcaca taaaaattct tttaggtgga
    33841 ggtcttaatt ttaataatat agaaaagata aaggaattga ctaaggcaag taattttcat
    33901 tttggtacag cgattagaat aaataacagt ccttttgaag atatagatag acaaaagctt
    33961 aaacaactcg taaatataat ttcaagatag tatatgaata taaaatactt agtttaaata
    34021 aagagagtaa gttaattata atttgggtat atgaatgtta ataggtatta aatatgaaat
    34081 tattgttgag tatgctttat aacttttaag tagttttaaa tataaataat gaaagaaaag
    34141 atagattatg gaggttttag agggtttata tttattaaaa aattataaag gtatattggc
    34201 atataataca cataatttcc taaaattacc atcttatgac atgaattcac atattaataa
    34261 acaattaatg gagatttttg tggatagaaa taggagtaaa aatagcttta aataaacaaa
    34321 aacaagagtt attaacagtt ttatccacat tatccacagg tgtttgtaat aatattaatc
    34381 cacataagta tcaatgtaac taaaaatgat aaataattct atcaattgaa tagaaaaaat
    34441 cataaaagta aataataaaa agaggattat gagagttcgt gtagaatata ttattaaagt
    34501 ataaattttc ttatactggc ataattgaag ggagatgtat ttatgaacat aattatatct
    34561 ggaaaacaaa tcgaattaac tgatgcaata aaaaataaaa tcgaaactaa actaagtaag
    34621 ttagataatt acattcatcc agacactgat gtaaaagtta cagtaagtgc gagaaaagca
    34681 agacaaaaaa tagaagtaac aatagctact gtaaatggtc ctataattag agcagaagat
    34741 atgcaggaaa atttatacac agctatagat gttgtatatg ataaattaag taaacaatta
    34801 aaaaaatata aaaagagatt acaagataaa cataaagaca acaagagtat aagattccaa
    34861 gatgcagaga taaatctagc tgatgaacaa gaggattatg gggatgaaaa tcaagaatta
    34921 gtaattcaaa gacgtaagaa atttagtgtt aagccaatga gtgaagaaga agctgtatta
    34981 cagatggaat taataggaca tgatttttat atgttcaaga acatagattc agatgaaata
    35041 gcagtagttt ataagcgtaa taatggtgga tatggtataa tagaacatga ataaagcaat
    35101 ttagatttaa tcatattagc tatcgataaa taaattatcg atagcttttt aaattaatat
    35161 taaaagtata gtttttatgg taaaatgaat aatagtacaa taaagaacga ggagagatgt
    35221 aaaatatgag ctttatggat aatttattca acatggcaga taaaaaagaa ttaaaaaagt
    35281 tcaataaaac tgttgatata atagattcat tagaacctaa atttgaatct atggcagact
    35341 cggaacttaa aaatatgaca aatatattta aagaaagatt agcaaatggt gaaagtatag
    35401 atgatatact tccagaagct tttgcagtag ttagagaagt ttctaagaga gtgttaggac
    35461 ttagacacta cagagttcaa atgattggag gtatagtact acatcaagga agaatagcag
    35521 aaatgaaaac aggtgaaggt aaaactttgg ttgcaactgc tcctgtttat ttgaatgcgc
    35581 ttactggaaa aggtgtacat gttgtaacag taaatgatta ccttgcaaaa cgtgatagag
    35641 accaaatggc aaaaatctat gagtttttag gaatgagtgt aggcgttatt atacacggtc
    35701 aaaatccaaa agttagaaaa gaacaatatg attgtgatat aacttatggt acaaataatg
    35761 aatatggatt tgactatcta aaggacaata tggtaataca caaagagcaa agagttcaaa
    35821 gaggattaaa ttatgctata gtggatgagg ttgactctat actaatagat gaggctagaa
    35881 caccacttat catatctgga cctggagaca aatcaactca cttatattca gatgcaaaca
    35941 catttgtttt aaccttaaaa ccagatgatt atgaattaga agaaaaagat aaggctgtat
    36001 ctctaacagc atcgggtata caaaaagcag aagtgtattt taatgttgat aatataacag
    36061 atatatctca tacagaatta taccatcata taaatcaagc tttaagagca catgtaataa
    36121 tgaagaaaga tgttgattat gttgctaaag atggagaaat agtaatagta gatgagttca
    36181 caggtagact tatgtttggt agaagatatt cagaaggttt acatcaagca atagaggcta
    36241 aagaaggtct taaaatacaa agagaatcta aaacactagc tactgtaaca ttccaaaatt
    36301 actttagaat gtacaaaaaa ctttctggta tgactggtac agcaaaaaca gaagaagaag
    36361 agtttaaagc aatctacaaa atggatgttt tccaagtacc tactaataag cttatgataa
    36421 gggaagactt accagactgt gtatacaaaa gtgaaatagg taaatttaat gctgtagcac
    36481 aagaaataat tgaaaggcac aaagtcaatc aaccaatact tgtaggtaca gtatcaatag
    36541 aaaaatcaga attattatca caaatactaa agaaaaaggg aataaagcac gaggtattaa
    36601 atgctaagca tcatgataaa gaggcagaaa taatagcaca agctggtaga cttggagctg
    36661 ttacaatagc tactaatatg gctggtcgtg gtacagatat agttctaggt ggaaatccag
    36721 actttttaac taagagagaa atgagaagaa atggatttaa agaagaaata gtaaatagag
    36781 tagatactcc aatagaaggt atacctgtaa aaggtaatga aattctattt gaagctagag
    36841 aagagtatga aaaactattt gaaaagttta agcaacaaac acaagaagaa caaaaacaag
    36901 ttgtagaagc aggtggactt gctataatag gtacagaaag acatgaatct cgaagaatag
    36961 ataaccagtt gagaggtcgt gctggacgtc aaggtgaccc agggagttca agattctata
    37021 taggtcttga tgatgacctt atgagattgt ttggtagtga tagaatatct ggtattgtag
    37081 ataaaatagg tcttgaagaa gacatgccga tagaacatag aatattatct aagtctattg
    37141 aaggagcaca aaagaaagtc gaaggtaaaa actttggaat aagaaagcat gtacttcaat
    37201 atgatgatgt tatgaataaa caaagagaaa taatatatgc agagagaaaa cgtgtattag
    37261 aaggtgaaga tttacaagaa caaatacaat caatgactca ttctataata gaagaagcag
    37321 ttactcttta tacacaagat aaaggatttg atgaagaagg attcaaagaa catatgtata
    37381 atttgttctt gccaaaaggt agcatagaaa taccagaaat agaaaaactt aatccagttg
    37441 aaataacaga aaaagtttat gaaatagcta tgaagatata tacttcaaaa gaagaacaag
    37501 ttggctatga gagaatgaga gaagttgaga gagtaatctt acttcaagct gttgataacc
    37561 attggataga ccatatagat gctatggacc aattacgcca aggtattggt cttcgtgctg
    37621 taggtcagca agacccagtt attgcttata aaatggaagg ttttgatatg tttgatgaaa
    37681 tgaataaaca tatcaaagaa gatactgtaa gatatttatt taatataaca atagaaactc
    37741 cagtggaaag aaaagcagtt gtagatgttg aaaatctatc ttctccatca gatggaacac
    37801 ttccaacaag taaaactgtt aaaaaagatg aaaaggttgg aagaaatgac ttatgccctt
    37861 gtggaagtgg taaaaaatat aaaaactgtt gtggaagata atctcggagg attaaatgtt
    37921 aaagttacaa gagtatagac atagtatgga gcagatggct tcaaatttag aagaattgag
    37981 ggtttctctt tgacatagct tcattgatga aaaatataca aattaatgaa gaaaagatga
    38041 atcaacaaga tttttggaat gataatgaag tcgctcaaag agtacttcaa gaaaataaat
    38101 ctttaaaaga gacattagaa gaatatgaaa gcttaaaaag cttattagaa gatatagaag
    38161 tattaattga aataggttta gaagaagata atgattctgt agaaagagat atagaaaagt
    38221 ccatagaatc aatggaagaa aaattatctg aaatgaaaat aaagactctt ctaaatggag
    38281 agtatgataa aaataatgct atattatcta taaatgctgg tactggtggc ttagatgctc
    38341 aagattgggc tcaaatgctt ttgagaatgt atataagatg gtcagaaagt aagggttata
    38401 aagttaagct tttagatata atatcagacc ctgaagcagg gataaagaca gctacaattc
    38461 ttgtagaagg aacaaatgct tatgggtatt taaagagtga aaaaggagtt catagattag
    38521 tgagaatatc tccatttgac ccatcaggaa aaagacatac ttcatttgca tctatagatg
    38581 taacaccaga gttagatgaa aatatagaag tagaaattaa tccatcagat ttaaagatag
    38641 acacatatag agcttctgga gctggagggc aacatgtaaa tacgactgat tcagcagtta
    38701 gaataacaca tatccctact ggtgtagtag ttcagtgcca aaatgaaagg tctcagcatc
    38761 taaataagga cagagctatg agacttttaa tggcgaaatt gatagaatta aaagagttag
    38821 agcaaaagga aaaaatagaa gatatacaag gcaaatactc acaaataact tggggtagtc
    38881 aaattaggtc atatgttttt caaccatata aattggttaa agaccataga acaaatgctg
    38941 agtttggaaa tgtagatagt gtaatgaatg gaaatataga cttgtttatt aatgaatact
    39001 taaaaatgaa taaaatagtt taataaatat gcactgtaat taagatatat gtataagaag
    39061 gttagtgctt aatatgcact tacagcactt aggctgagga cgataaccct cagccttcgt
    39121 tctgttttgt tatacttatg aaaaactaac attaggagtg aataaattgg atataaatca
    39181 aatcttaaaa aaagagttta atcttagaga tgaacaaata aataatacat taaaacttat
    39241 tgatgaagga aatacaatac catttatagc tagatataga aaagaaatga ctggcgaaat
    39301 gagtgatgtt acactaagag aattttatga aaaattaatg tatttgagaa atcttcaaag
    39361 tagaaaagat gatgtagtta gactaataga tgaacaaggg aaattaacag atgaaataac
    39421 acaaaatata gaaaaagcaa aaacattaca agaagtagaa gatatatatg ctccatataa
    39481 acaaaagaaa agaactagag caacaatagc aaaagaaaaa ggattagaaa atttagcatt
    39541 gtcaatacta gagaataatt tagataatat agaaatagag gcaaagaatt atcttgatga
    39601 agagaaagaa gttctttcta ttgaagatgc tctaaaaggt gctagagata taattgcaga
    39661 actagtctca gatgatgcta agattagaaa gtatataaga gaattagctt taagagaagg
    39721 tatgatagtt agcaaaagtg ctactgatga gaaatcggtt tatgatatgt attatgatta
    39781 tagcgaagca gtaaaaagta tggctccaca tagagttttg gcaataaaca gaggagaaaa
    39841 agaaagcttc ctaaaggtta agttggaaat aaataatgat aaagttttaa attacataat
    39901 aaacgaatat gtaaatgata aaaatttcaa aaataaagaa gaaatcgtaa gttcaataga
    39961 agattcttat aaaagattaa ttttcccatc aatagaaaga gaaataagaa atcatttaac
    40021 agaaatagct caagaaagag ctataagtgt atttggaaag aatgttaaaa gtttattgct
    40081 tcaaccacca gttaaagaca aagtagttat gggatttgac cctgcattta gaactggatg
    40141 taaaatagct gtagtagata aaaatggtaa actactagat tatacaacag tatatccaac
    40201 agaccctcaa aatgatgtag agggagcaaa gaaagtttta aaaggattga ttgaaaaata
    40261 cgatatagat ataatttcaa ttggaaatgg tactgcatct agagaatcag aaacttttgt
    40321 atcagaaatg ataaaagaaa tagatagcga agtgcagtat gtgatagtta gtgaagcagg
    40381 agcatctgtt tattcagcat ctgaacttgc aaatgaggaa catccagata taaatgtatc
    40441 aataagagga gctatatcta tagctagaag attacaagac ccattagcag agcttgtaaa
    40501 aatagaccct aaaagtattg gtgttggtca atatcagcat gatttaaaca aaaagagatt
    40561 agaagaagta ctagatggag ttgttgaaga ttctgtaaat agtgttggag tagatttaaa
    40621 tactgcatca tactccctac tagaacatgt tgcaggaata agtaaagcta tagcaaaaaa
    40681 tataatagca tatagagaag aaaatggaga ttttacgtca agagcacaat tgaaaaaagt
    40741 aaaaagacta ggacctcaag catttactca atgtgctgga tttatgagaa tcttagaggg
    40801 taaaaatcca ctagataata caggtgtgca tcctgaaagt tatgatatat gtaaaaaaat
    40861 gattgagata attggatatt ctttagatga tgtaaagaat aagaatatag gtgaaataga
    40921 tgaaaaaata aaagaaattg gcttgaggga attaagtgaa aaactggaag taggacaagt
    40981 tacgttaaaa gatatcatag ctgaaatcaa aaaaccagga agagacccaa gagaagaagg
    41041 tataaaacct atacttagaa ctgatgtctt aaaaattgaa gatatacaag agggtatgac
    41101 tttaaaaggt acaataagaa atgttgtaga ctttggtgca tttgtagata ttggaataaa
    41161 aaatgacgga ttagttcata aatcagaaat gagtaatagt tttgtgaaag accctatgag
    41221 tattgttaca gtaggtgata tagtagatgt aaaagtcatt ggaattgact taaacaaaaa
    41281 aagagttgcc ttatcaatga aaaaatagat aagtttatgt atggttatag tacttagatt
    41341 ctatatttgt aaataagatt actcgagtgt ataaatgtta aaaatttgta aaagtatata
    41401 gtaagaaaat atattaaaat aagaacgata gtcttgaaaa tcaggaggaa aatgaatgat
    41461 ttttataaaa aatggtaaaa taaataccat aacaaatggg ataattcatg gagatatact
    41521 tatagatgaa ggaaaaataa tagaaattgg agaagatttg atagctcctt tagatgtaga
    41581 ggtaatagat gctagtaata aacttgtgtt tcctggattt atagatgctc atactcactt
    41641 aggtttatgg gaagatggta tagggtttga aggagcagat ggaaatgaag agactgaccc
    41701 aataacacca caattaaatc ctatagatgg aattaatcca atggatagaa cttttaaaga
    41761 agcttttgaa ggtggaataa cttctgtttg tacaacccct ggaagtgcaa atgttatggg
    41821 tggtcaatgt atagctataa agacatgtgg aaaaagaatt gataaaatgg taataaaaaa
    41881 tccagttgca tctaaaattg cttttggaga aaatccaaag agctgttatg gtcaggatga
    41941 taaatctcct caaactagaa tggctatagc tgctttactt agagaaaacc taaaaaaagc
    42001 agaagaatat ttagaggata ttgatatgta tgagagtcat gatgatgaag actgtgaaaa
    42061 acctgaatat gatataaaaa tggaaagttt aataccagtt ttaagaagag aaattccttt
    42121 taaggcacat gctcatagag cagatgatat gtttacagca ataagaattg caaaagaatt
    42181 taatttaaag cttacattag accattgtac agaaggtcat cttattgtag atgaattagt
    42241 ggaagaaggg tttcctgtaa tagtagggcc atctttgtca gaaaggtcta aatttgagct
    42301 tagaaatttg acatttaata cagcaggaat actttcaaat gcaggtcttg atgtatgtat
    42361 aatgacagac catccagtaa taccagttca atatcttcca atatgtgcag gtattgcagt
    42421 aaaacatggt atgaaagaag aaaaagcaat agaatcaatt acgataaatc cagctaaggc
    42481 tttaggaata gaagatagag taggctctat agaagttggt aaagatgcag acctagtaat
    42541 ttgggataat tctccactag agattcaaag taatgtactt tacactataa taaatggcaa
    42601 agtagtgtat gagaaaaaat aacttgacaa ttattaaaaa attaactaat ataagagtgt
    42661 aaggaaaatt aaaaaatcaa agaagatctt cggggcaggg tgaaattccc taccggcggt
    42721 atagcccgcg aactacttag gtggttgatt tggtgagatt ccaaagccga cagtaaagtc
    42781 tggatgagag aagatattta tggaagataa ataagtttat tttagtttgt ttaaactata
    42841 tccataaaaa ctcgaagata gggtcttcga gttttttgtt tttcctaaaa aatataaaca
    42901 tattgattag ggggaaaata attatgcaaa acacagcaaa aagaggcaat atgatttcaa
    42961 caagaacttt agtagtaatg tcaattttat ctgcaatatc ttatttatta atgtttattc
    43021 aagcaccaat accaggtata tttccagatt tcttaaaaat tgacttatca gatattccag
    43081 caatattcgg aggaatgtct ctaggaccat tagttggatt tggaatagtt gtagtaaaaa
    43141 atgtgttaca agcaataaca gcaacaacaa ctggaggaat aggagaattt gcaaatatat
    43201 taattggtgg ttcttatgta atgatacttt gttattcata taagagaagt ggagatttaa
    43261 aaggtgtact gattggtttt gtattaggaa caatatctat gactataatt ggttcattag
    43321 taaattattt tgtaatgttg ccactttatg gtcaacttat gggactagat gctataatag
    43381 gtttaggttc agctataaat cctcaagttc atgatttatt cacttttgta atttggatga
    43441 tagctccatt taatgtgctg aaggctgtta taatatcttt agtaacattg ccactttata
    43501 aaaaaatggg aaatataata aagaaataat tagacaagaa aatataaaat taaatgtatt
    43561 gaatagccat aatttattta taacttcagt cctatattgt gattgatata ttaaatatat
    43621 tatggcttta ttaatttatc aaaaaatgta tataaaataa gaaatattta gacaaattta
    43681 aattttaaaa taaaggaaga attaaaacga ttaactatta aaatatgaaa aaggtatcta
    43741 caatatacag agcgaattta taataaacaa aaaataaagt tattattaaa aaactataaa
    43801 gattaataat caaactttta ataataacta atttaataat gataggggtg tatattcatg
    43861 catttatcta aaaaaataaa aaaattattt ttagatgata aagggtatta ttttaatagt
    43921 aattggaaag atattcatat tggtatttat gatacaaaat taggagattc tttaaagtat
    43981 atcactaatg aatatattga aaatatggaa agggtaaaat acgattatcg tgaattaaaa
    44041 aatatagaaa atcaaactga tggtatgtgt attttagcaa taaggcagaa ttacaaggca
    44101 ttagagtatg taaaaaatca aaccgaagaa ttatgtttag aagcaataaa gtataattat
    44161 aaagcacttg aatatgtaaa agaacaaaca gaatacttgt gtcttgaagc tataaaaaaa
    44221 gattgtaatg cacttaagta tgtacgaaat aaaacagaag ggcttattat aaaagcaata
    44281 agtcattctt ccaatataga tgttgtaagt atactaaagg ctttagagac tcaaacaaga
    44341 aggatatgtt tagaagcaat taaaaaagat ggcaggtgtc ttgcatacgt tagagaacag
    44401 agtgaagaat tatgtataga ggccattaag caaaattata aagcattaaa atatgtaaaa
    44461 aatcaaacgg aaaaaatgtg tatagaatcc gtaagacaaa atggtatggc attacaatat
    44521 gtaaataaac aaactgataa aatatgtata gaagctgtca aacaagatgg aagaagtcta
    44581 caatttgtca acaataagac agaagaaata tgtataaatg ctataagata tttaaataaa
    44641 aaatataata ttaaagatgt actaagttac atagataagt atacagaaga tatatgtata
    44701 gagattgtaa gacaaaatgg aaaaatgctt atgtatataa aaaatcaaac agaaaaaatg
    44761 tgtattgaag cagtaaaaga gaactataag tctcttaagt atgtaaaaga gcaatctgag
    44821 agaatatgta aagaagcatt gaagcaaaac cataaagcaa aagagtatgt aaaaattgct
    44881 attgatgatt gtatataagg tgtagataca gccatcaata gtgaaagata aaaaaagata
    44941 aagacaagta ctttataata atctaggctt atactataat taattaagca gtgatataaa
    45001 ttcacatgac gattatatca aaatggagat gaattgatgg caaaaatata tttagaaaat
    45061 gaaaataaaa ctagagaaat aggatataaa ttaggtaaac ttttaaaaga aggcagtgtt
    45121 atctgtttag taggagattt aggagctggt aaaactacaa tgacacaaag cttggcagat
    45181 tcattaggga tagaagatta tataacaagt cctactttta ctataataaa tgaatatgaa
    45241 ggaaaaatac ctttgtatca ttttgatgtt tatagaatag gtagtagtga tgaaatgtat
    45301 gatattggtt atgatgaata tgtaaattca aatggaatct gtataataga atgggctaat
    45361 ctaatagaag atatacttcc aaaagaatat ttgaatatag aattgagata taaagatgaa
    45421 ggaagagaaa tgatattaac tccaaaagga gagttttata aagagatagt tgaggagttg
    45481 attaaataat gaaaatacta ggtatggata cgtcttctat ggcagcaagt gtagcagttg
    45541 ttgaagatga taacttaata tgtgagttta cagtaaacaa caaaaaaact cattcacaaa
    45601 agcttatgcc aatgatagaa aatatgttga gtatgagtga tttaagtata aaagatatgg
    45661 atttattagc tgtatgtata ggtccaggtt cgtttacagg acttagaata ggtatggcta
    45721 cagtaaaagc tatggcccat gtaaataata taccaataat agcagtaaat tctttggaat
    45781 ctctagcaaa taacataaat ttctgtgata gaaagatatg ttgtatatta gatgcgcaga
    45841 aaaatcaagt ttattcagct aaatacaagt ttgaaaatgg agagatgata gaattagatg
    45901 gtgttgatgt tgtagagttt gatgctttag tagatgaaat tgtatcaact aatgaagaat
    45961 ttataatagt tggagaagct gtatacaaat ataaagacaa gttagagaat ataaaaaata
    46021 taaaaatacc ttcaccagct aataatgtat ctaacgcagg aagcttatgc tctttagcat
    46081 taaataaata taataaaaat atagatgtac atacttgtta tacgataaat cctatgtata
    46141 tcagaaaatc tcaagctgaa gtacaatatg atgaaaaaat gaagaggcta aataatggaa
    46201 gataatacaa aactaatagt agatgatata aaaatagaag aaatgactac agaagatata
    46261 gatgaagttt ttgaagtaga aaaaaattgt tttgaagatt attggtcaaa agagtctttt
    46321 agaaaagagt tgtctaatga agtagcaagg tatatagtag ctaagttaaa tggaaaaatt
    46381 gtcggatatg tgggaatttg gcttatactt gatgaagggc atataaataa tgtggctgtt
    46441 catagtgact atagagggaa aaaaataggg gacaagttga taaaaggcat agttgattta
    46501 tgtaaagata ataatattgc atctatgaca ttagaagtta gagcatcaaa taaaatagca
    46561 caaaatttat atagaaaata tggatttaaa atgggtggaa taagaaaaga atactacagt
    46621 gataataaag aagatgcaat tataatgtgg aaccaattaa aagaggtgta gagtttatga
    46681 gtgatataat aacattagct atagagagta gttgtgacga aacagctgca tcagttttaa
    46741 aaaatggtag agaagtactt tcaaacataa tctctactca aatcgagaca cataaaaaat
    46801 ttggtggtgt agttccagaa gtggcctcaa gaaaacatgt ggaaaatatt gatatagttg
    46861 ttcaagaagc tttagacaaa gcaaatatag gttttaatga tatagaccat atcgcagtta
    46921 catatggacc tgggctagta ggtgctttat tggttggtct atcttatgct aaagcacttg
    46981 catatacttt gaatattcca cttgtaggag taaatcatat agaagggcat ctaagtgcaa
    47041 actatataga gcataaggat ttaaaacctc cttttataac attaatagta tctggaggtc
    47101 atactcactt ggttgaagta aaagattatg gcaagtatga aattttagga aaaacaagag
    47161 atgatgcatc tggagaagca tttgataaga tttcaagagc tatgaattta ggctatccag
    47221 gaggtcctat tattgataat ctggccaaga atggaaataa acatgctata gaatttccaa
    47281 gagcctattt agaagaagat agctatgatt ttagttttag tggattaaaa tcctctgtat
    47341 taaattatct aaatggaaaa agaatgaaaa atgaagaaat agtagttgaa gatgtagcag
    47401 catctttcca agaggctgta gtagaggttt tatctactaa agcactaaaa gcagtaaaag
    47461 acaaaggata caatatcata actctttctg gtggggttgc ttctaattcg gggcttagag
    47521 ctaaaataac agagcttgct aaagacaatg gtattactgt taagtatcct cctctaattt
    47581 tatgcacaga taatgcagct atgatagggt gtgcaggata ttataatttt ataaatggga
    47641 aaacgcatga tatgagttta aatgcagttc caaatttaaa gataaatcag tagagttaga
    47701 tgaatttgta aatataaaag tattgtttca aaaaagtaag tagaagtgaa attaaatata
    47761 ataaaaataa atatagtata tattaaaact tgaagtaaaa ctatttataa tctttatatt
    47821 ccaatgaatt gcaacaaatt ttaggatata cagattgtct ttggcttaga tttgcttcaa
    47881 gtttaatttt ttctatagaa agattatttt aaaaagtggg taatataccc ttttttttca
    47941 ttcttaaaaa gttttatact aattatagaa agattaagga ggggaagaaa tgagtcaaag
    48001 taaagaagac aaaataagaa gtattttgga agcaaaaaat attaaatcaa attttcaaaa
    48061 taaagaaaat ttaagtgaat ttaatgaaaa aaaagcaagt aaaagagcag aagacttatt
    48121 agatgtatat tataatacat tatctactgc tgatatggaa tttccatatt ggtataatag
    48181 agaatataga aaatcagatg gtgatatacc agtagttaga agagctaaag ctcttaaagc
    48241 tgctttttca catatgacac caaatataat acctggtgag aaaatagtaa tgcaaaagac
    48301 acgccattat agaggttctt ttccaatgcc atgggtaagt gaaagtttct ttgttgctca
    48361 aggagaacaa atgagagaag aggcaaagaa attagccagt aacacagctg atgaacttac
    48421 aaaatttggg tctggtggag gaaatgttac tgaaagtttt ggaaatgtcg tttcaatagc
    48481 tggtaaattc ggaatgagaa aagaagaagt acctgtactt gtaaaaatgg caaaggaatg
    48541 ggttggaaag tctgttgaag atttaggttt tcactatgaa aaaatgatgc cagattatga
    48601 tttaaaagaa aacctaatga gtacacttat atgcatgttt gattcagggt atacacttcc
    48661 tcaaggtaga gaagttataa attattttta tcctttaaat tatggattag atggaattat
    48721 agaaatggca aaagaatgta aaaaggcagt tgcaggaaat gcatctggag atgggcttat
    48781 tggtatggac cgtctatatt tttatgaggc tgttattcaa gttattgaag gattacaaac
    48841 ttggatttta aattatgcaa aacatgcaaa atatttagaa agtatagaga cagatttaga
    48901 agctaaaaaa gaatactcag atttagtgca gatattagaa catattgcac ataaacaacc
    48961 tcgtacattt agggaagctc ttcagttgac ttatacaatt catatagcat cagtaaatga
    49021 ggatgctata tctggtatgt ctataggacg ttttggtcaa attttatatc cttggtatga
    49081 acaagatata gaaaaaggac taattacaaa agaagaggtt atcgaattat tagagcttta
    49141 tagaataaaa atcacttgta ttgactgttt tgcttcagcg ggcgtaaatg gaggagtact
    49201 atcaggcaat acatttaata ctttatctat aggtggatta aaagaagatg gttctacagg
    49261 agctaatgaa ctagaagagt tattattaga agcaagtatg agatgtagaa ctcctcaacc
    49321 tagtttaact atgctatatg atgaaaagct tccagaagat ttcttaatga aagctgctga
    49381 atgtacaaag cttggttctg gttatccagc atgggtaaac aactcaaatg gaacaacatt
    49441 tatgatgaaa caatttgctg atgaaggtat gactgttgaa gaagcaagag catttgcatt
    49501 aggtggatgt ttggaaacgt ctccagggtg ttggaaacag ttaactttaa acggaaaaac
    49561 atactctatt gcaggtggag caggtcaatc tgctggctct ggagttcact ttatagctaa
    49621 tccaaaaatt ttagaacttg ttcttatgaa tggtaaagac tatcgtatga atatacaagt
    49681 atttgagcct cataataagc cattagatac ttatgaagaa gtcatagaag tatttaaaga
    49741 ttattataaa caagcaataa atgttttaga aagagcaaat aacattgagt tagatatatg
    49801 gagaaaattt gatacttcta taataaattc actactaaag ccagactgtt tagacaaagg
    49861 tcaacatata ggaaatatgg gatatcgtta taatgcaaca ttaaacgtag aaacttgtgg
    49921 tactgttact atggtcaatt catttgcagc tcttaagaaa cttgtatatg atgacaaagc
    49981 atttacaata gaggaaatga aggatgccat attaaataat tttggattta aagatgcatt
    50041 agaagtagga aattattcta tggcggacca agtaaaagta gataagactg gtaaatatga
    50101 tgctatttat aaggcctgtc ttgatgcacc taaatatgga aataatgatt tgtatgcaga
    50161 taatattctt aaaaactatg aggtatggtt atcaaaagta tgtgaagaag cacaatcttt
    50221 atatgctaag aagatgtacc cttgccaaat atctgtttct acacatgggc cacagggagc
    50281 tgctaccttg gctactccag atggtagact aagtggaaca acatattcag atggttcagt
    50341 ctctgcatat gcaggaacag ataaaaatgg agtatatgca ttatttgaat ctgcaactat
    50401 ttgggaccaa gcagtagttc aaaattctca gatgaattta aaacttcatc caactactat
    50461 aaaaggacaa caaggaacta aaaaattatt agatttaaca agaagttatt taagaaaagg
    50521 tgggttccat attcaatata atgttgtaga ttcagaaaca ttaaaagatg cacaaaaaaa
    50581 tcctgacaat tatcgtcaat taatggtacg tgttgctggg tttactcaat attggtgtga
    50641 attaggtaaa ccaatacaag atgaagtaat tgctagaaca gagtatgaag gggtgtaaaa
    50701 tatgagaaag catagtgatt gtatgaattt ttgtgcagta gatgcaacca aaggaatttg
    50761 tagattatca aaacaaatga ttaatttaga tgatgcagca tgtccagaga taaaagtaat
    50821 gccaaaatgt aaaaattgta aaaattttgt tgaagctaat gatgaaggta taggcaaatg
    50881 tgttggccta gagaaagaag actgggtata ttcaacattg aatgcaatta cttgtgaagg
    50941 acatgtgttt aatgagtagt caaaagcaat tagaaggcat gatttttgat gtacaaagct
    51001 tttcagttca tgatggtcca ggttgtagaa caactgtatt tttaaatgga tgtccactga
    51061 gttgtaaatg gtgtgcaaat ccagaaagtt ggactgtaag accacatatg atgtttagtg
    51121 aattatcttg tcaatatgaa aatggatgta ctgtatgtca tggtaagtgt aaaaatggtg
    51181 cattgagctt taatcttgat aataagccag ttatagattg gaacatctgt aaggactgtg
    51241 aaagttttga atgcgtcaac tcatgttatt ataatgcgtt taaattatgt gcaaaacctt
    51301 acactgtaga tgaacttgtg caggtaatta aacgtgattc taacaattgg agaagtaatg
    51361 gaggagtaac atttagtgga ggtgagccac tacttcagca tgaattttta catgaagtat
    51421 tattaaagtg tcatgaggta aatatacaca ctgccattga aacgagtgca tgtgtatcaa
    51481 atgaagtttt taataaaata tttaacgata ttgattttgc atttattgat ataaagcata
    51541 tggatagaga aaaacacaaa gaacaaacag gtgtgtataa tgatttaata ttagaaaata
    51601 tatcaaacct tgcaaattct gattggaatg gaagattagt acttagagta ccagttatct
    51661 ctgggtttaa tgatagtgat gaaaatataa gtgatattat atcttttatg cacaaaaata
    51721 atttggtaga aattaatcta ttaccatttc acagattagg agaatcaaaa tggattcaat
    51781 taggcaagga atatgagtat tctgataagg gtgatgttga tgaaggtcat ctagaagaat
    51841 tacaagatat atttttggat aatggtatag cttgttatgt tggccatgag acagctttct
    51901 aaaattaaat acaagtttta attaaaaaag gatgttacac taaaagctag agttacatcc
    51961 ttttttatgt ggtttttatg tagaaataat taagtaaagt tttatatgtt ttatatattg
    52021 tttatattgc tttatgtcca taaatattgc tttaaatata ctttatatat tttttatagt
    52081 ttaaattaca gttagagaat atattacgtt tacttcattg aagcagaagt tttgtggtaa
    52141 aatactacta tgaaaaaatt taatagaata tattttagaa acaactaaaa ataatagtta
    52201 ggaggaagac atctatatga acaaatcatg gagcaacttg attattaaga ataaagacag
    52261 aaaagtacgt tcagggtgga ttattatagc tgttatggct accttttaca tacttcagta
    52321 ttatttaagc atgatacttg tggaaataat aagaaaaatc ctggttgcta caggtgacat
    52381 taatttaaaa acaaactatt tttcagaata tgctaattgg ttaaatgatg ttgggttaca
    52441 gattgtattt caaattctaa cagattcact tatgattatt attcctataa ttgtatggag
    52501 atttattatg aaaaatccta ttagtgaaat gggattacat tcttatagaa ttaagaaaaa
    52561 agaaggatat gtaggaatgt tattaggaat atttaattgt acagtaatct ttattataac
    52621 aataacaatt ggtggtggtc atgttacttc atgggtacca aaaatttcac ctttaacagt
    52681 ttcttggata ctggtcttta ttttagttgc ttttgcagaa gaaattttaa atcgtggttt
    52741 ttttatggca gttttacgaa gatgtaaaaa tatttatttt ataatgattg ttccatctgt
    52801 tatttttggg ttgattcata tatggaatcc tgatgttact tttttgtctg taattaatat
    52861 tataattatt gggattcttt tttcatatat gtttataaaa tcgtcgaata tatggatgtg
    52921 tataggttat cattttgcat ggaatgtatt tcaggggatt atatatggta tgcctgtaag
    52981 tggacttcaa gttccaggga ttataactac acaaatcact catggtaatc tattaaatgg
    53041 aggaatgttt ggtattgaag gtggaatttt gactactttt gttaatctac ttagctttat
    53101 atttgtatgg tattactata gagattctaa gtatgatttt gttagtgata aagtaaatgt
    53161 gaccaatatc aagaattagt tgattataat aaattttaat aatatcttct aagcttaaag
    53221 tattctgtag gattgttgga aatttgcaaa aatataaaaa taacttggat attctattta
    53281 ctccaagtta tttttatatt cttttgtaaa tagaaatgga acataaaaaa ttaaaacaac
    53341 catagatact attagtatat catggtaagt gatagttgat tctttagaat ttgtgataag
    53401 aaaagcacta ttatttaaaa catgaattat agcacaaaac cagatattct tagtttttat
    53461 ataaagatat cctaaaaata ttgaaattcc aactatatgt attgaacgta atattattcc
    53521 taatattggc gcttcaggtg tatataatgt aaatattagt ggtaagtgcc acaagctcca
    53581 acacatacct aatattataa ctcctaactt tttaccaaat ttatcaaaaa aaatattctg
    53641 taaaaaacct ctccaggcat attcttcact aaaaaaaata taaatagtag ttaatgatgt
    53701 tattggagta aatataaatt ttatgatacc actataatta ggttgagccc cttgtagtat
    53761 tagtataaat gatgttggaa caattacaga aatcataaag taaataatta ataaagaacc
    53821 tagtttaata ttttttacag gatataaatt ttttttattt gatgaatata ttataattga
    53881 taagctactt ataaaagtta atattaggtt tataattata aattcattat tatttattag
    53941 atttaataac ttaagaacaa ttaataatga ataaataaga aaaaataata tgattagtga
    54001 ataaaattca tacatatcat tatttaatcc ttcagtataa aactttgcaa tggcaactga
    54061 agatagaggt aatatcatca tgaatgatgc aaagttttct ggatcaataa atttattata
    54121 atataacagt aaacctaatg aataagttaa accaaagttt ataaagaaaa agataataat
    54181 ttgtattttt tttcttttta tttcattttt aataaatact gggtttagaa tatttatcca
    54241 tattaatttt a
//
LOCUS       DAELLV010000005        47680 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.005, whole genome shotgun sequence.
ACCESSION   DAELLV010000005 DAELLV010000000
VERSION     DAELLV010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 47680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47680
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.005"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            32..1960
                     /locus_tag="KPD58_000262"
     CDS             32..1960
                     /locus_tag="KPD58_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG0264053.1"
                     /translation="MHKRLLTLFKLLNESDDKITCKTLSNHLKVSERTIRNDITSING
                     TLEKNGAIIKIKKGEGYYIDILNLALYQQYLALISDDIMDSSEIPDSPIERNQYILKY
                     ILYNNTYIKLEDLANSLYVSKFTILNDIKRIKPILSKYNLILVSKPYYGVKVEGKEID
                     IRRCISNNMINRNFENYIIGITDREIELFNNVDLIELQRVVLSEINKFNINFLDFNLK
                     NFIIHLAITVSRILDGYCLDNVLDVVLTDFQSNTTVENIFNYIESKYTIIISKADRVY
                     LYNHFITKSSLLDNVSNRVDTKIIEYVEEILEVINNQYTFDLRNDSVLFDDLVLHFKS
                     ILNSKSYNLNKVNPLINTIKSNYPLAFEITLNAIEKVFKNSIYSLTEDEIGYVSLHIG
                     AGIERFFQSNIKCKNVVLVCGSGYGSSRLLEVQLNKVFHDKINILQCLSFNQFLASEL
                     SDVDIIISTIPLNHDSIPIVLVDLKLLKKDIENISKSITNNSHIYSNLLDNFFDKNLF
                     IVNPKIKDKDELIKLMCNKLTQSEIVFPSFAESVFYRESLSSTNIDDFLAIPHPMELS
                     SIRTKICISILNEPIYWSENSTVKLIMMLAINKDDYIKINSIYDILLKIIHDNDIRDS
                     ISNCNDFDNFLSIIKSIV"
     gene            complement(2220..2981)
                     /locus_tag="KPD58_000263"
     CDS             complement(2220..2981)
                     /locus_tag="KPD58_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0264054.1"
                     /translation="MEYTVQKLSKLAGISTRTLRYYDEIGLLKPLKINSSGYRIYGQN
                     EVNKLQQILFYRELGINLENIKNIINSPNFDSLSALKEHHNKLLAKRKQIDLLIENVT
                     KTIALKEGKYTMTDIEKFEGFKEKMIDENEKNYGTEIREKYGKDIIDTSNKKLKNMSK
                     QDYENWQNLNIEIISKLKKAFKTGDASSELAQEVAKLHHKWLCYTWNTYSKEAHSSLA
                     QMYVYDERFTSYYDREQPGLAKFLRDAIVIYTRDK"
     gene            3200..3676
                     /locus_tag="KPD58_000264"
     CDS             3200..3676
                     /locus_tag="KPD58_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBG0264055.1"
                     /translation="MNLKRSNEKREEYRRMYSDKESFLSLIQNFTSVSIAKELTLKNI
                     ELETSFICEYKGKEVDIIYKVFSKSGKVSHYIVLEFQTEMDTEIVPRLKSYREQIWKS
                     FIMKKSLEEIEDKNFKLPKVIPVVLYSGPERLSRKKGILDIVESISEFSNNSSMKN"
     gene            3774..3926
                     /locus_tag="KPD58_000265"
     CDS             3774..3926
                     /locus_tag="KPD58_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate translocator"
                     /protein_id="HBG0264056.1"
                     /translation="MRNNKSKVLLGILCLATGMAVILSMLLPGWIWSALTALILIGCG
                     ALLFFC"
     gene            complement(4086..4274)
                     /locus_tag="KPD58_000266"
     CDS             complement(4086..4274)
                     /locus_tag="KPD58_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBG0264057.1"
                     /translation="MAKVCSVCGKGKVSGNQVSHSNKHNKRTWSANLRSVRAIIDGAP
                     KRVKVCTRCLRSGKIERA"
     gene            4635..4985
                     /locus_tag="KPD58_000267"
     CDS             4635..4985
                     /locus_tag="KPD58_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBG0264058.1"
                     /translation="MSAKVTNQYGNIEIDNQVIAQVVYRAAMESYGLVGLAYKSKGIV
                     ELLKGENATKGVRVEEVEEDAIAIELYVIIQYGTNISTVANNIIDRVKYVVEKMTGVR
                     VTRIDINVQGIRVK"
     gene            5004..6647
                     /locus_tag="KPD58_000268"
     CDS             5004..6647
                     /locus_tag="KPD58_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HBG0264059.1"
                     /translation="MIQYIDGKRLREMFISGANNLQNNKELVDKLNVFPVPDGDTGTN
                     MSLTISYALKELAKVENDNISDIGKALSKGSLMGARGNSGVILSQIIRGIAKSIEGKS
                     KLSTEDLAKAFKNGSDTAYKAVIKPIEGTILTVVRESGEFAIKTAKKEKDVVKFLSML
                     VKESNSSLERTPDLLKNLKEAGVVDSGGKGLVLIYEGMLASIKGDNIEIKNVDLDTNI
                     STSMDFAKSTTSTDDIKYCYCTEFILESSKVEDTKIRDIMMAYGDSLAVVGDDGVIKV
                     HVHTNDPGNVLQEALKYGQLLTIKIENMKLQHENTLLDVEEKKENDSEPLEEEKEFGF
                     IATSMGEGLANIFKDFGVDHIIEGGQTMNPSTEDFMNAIKHINAKNIFIFPNNSNIIM
                     AANQAKELSDKNIIVIPTKNTPQGFAALVTFNGELSEDENKEAMMNALNSVKSGQVTF
                     AVRDTVMNEIDVKEGNIIGIAEGNLLSAGDYVDEVTSNLIEKLVDEDTAIITLFFGED
                     VTESQANELRTSLEEKFEDVDVELYYGGQPLYYYLISVE"
     gene            6728..8785
                     /gene="recG"
                     /locus_tag="KPD58_000269"
     CDS             6728..8785
                     /gene="recG"
                     /locus_tag="KPD58_000269"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBG0264060.1"
                     /translation="MDLYKDVQYVKGIGPKKADKLNKLGIFTLKDLLYYFPRQFEDRN
                     NLKKIAQLEDGEKVTIKAVISSINTFSPKEGMTLTKIDVKDETGSAKLVFFNKSYIKN
                     TFRPGDSILVFGKVKKKFNNLELTSCELEYLTNSPKNTCRFMPVYQLTYGVTNKEIMS
                     IIRTVLEDKELIIQEYMPQRIIEKYRLCSIDFAVRNIHSPSSKESLKIALYRIVFEEL
                     LILQLGLFVFKSGRNKEDGIKFETSKDLKKIISALPFKLTKAQNRALDEIIQDMNLEK
                     IMNRLVQGDVGSGKTVVALLALANCVLNGYQGALMAPTEILAGQHYISLTESLKDFGI
                     NVGLLIGSLTKKQKDTVLEQIKNNEIDILIGTHALIEDKVEFNNIGLVITDEQHRFGV
                     MQRSKLSLKGANPDILVMTATPIPRTLALILYGDLDISIIDELPPGRQPIETIAIEKS
                     KRDRAYNNLVRREVESGRQVYIVCPLVEESEAIEAKSAVELVEELRAEYFHDLRLGLL
                     HGKMKSSEKDEVMRLFKDKEIDILVSTTVIEVGVNVPNATLMIIENAERFGLAQLHQL
                     RGRVGRGSHKSYCVLIYDSKTDVCRQRMAIMEETNDGFKISEKDLEIRGPGEFFGTRQ
                     HGLPELKVANLFKHIKILKLAQQEARYILGEDNNLQLKENMALKKEIIDKFKDTLKEI
                     SLN"
     gene            8844..9398
                     /gene="rsmD"
                     /locus_tag="KPD58_000270"
     CDS             8844..9398
                     /gene="rsmD"
                     /locus_tag="KPD58_000270"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HBG0264061.1"
                     /translation="MRVISGKARGLKLNTPKNEDVRPTTDRVKESLFNMINSYIMESE
                     VLDLFAGTGSLGIECLSRGAKSCTFVDISKESIDIVKSNIKKARVESESIILNLDFKT
                     AIDKLKLQNSKFDIIFMDPPYYKNMFIEAIEKIDNSNLLNEDGIIVVEHDTNDLFPDK
                     ICKLEKTRDKKYGNTTLTFYKMEA"
     gene            9400..9897
                     /gene="coaD"
                     /locus_tag="KPD58_000271"
     CDS             9400..9897
                     /gene="coaD"
                     /locus_tag="KPD58_000271"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBG0264062.1"
                     /translation="MENKPRKAIFAGSFDPITNGHLDIICRASKLFDELQIGVLNNPN
                     KKGLFSFDERVKLIEKSTSHLNNIKVVTFDGLLINYCQENGIGALVRGVRSGADVDYE
                     LQMAHMNRELNPDIETIILPSCTKYSFISSSLIKEVLLFDADIKNLVPKIVLEELKKK
                     TSGGN"
     gene            9901..10425
                     /locus_tag="KPD58_000272"
     CDS             9901..10425
                     /locus_tag="KPD58_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264063.1"
                     /translation="MKIDLEMIELFEQLEEIFRSASTIPFSHKCTVDKDEVLAIINDI
                     RTLIPEEVTQAVWINKERNKIISQAKQDATNIVEQAKKEAERIQQEYQDNIEELKKNS
                     EDVVKAYVESSEPVVQADQRAKDIVDRAERIANEIRIGSIEYAEDVLSSVEYNLREIL
                     EEVKRDKAELRPKK"
     gene            10769..12688
                     /locus_tag="KPD58_000273"
     CDS             10769..12688
                     /locus_tag="KPD58_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG0264064.1"
                     /translation="MKERAKKILDFIIKNNNVTSQELQEKFNVSKRTIYYDILAINEQ
                     LGKSGNIKNVKHKFIFEGNLCDARKIISTEEDKFLDSDYRKTFILNKILLGEKISIEK
                     LTNEMLLSKNTVVQTITDVKKYLQTMGLRLEYKGKYKIIGDEYVIRELFLIIVQENIL
                     EINSISEEVSSFDTKGHIKLTDYSLLNLTKFVEFLNKRIRDGKTLYSYKYLNEAKKIS
                     YFSNCKELLCEEANENEQAYICTYISSLPSLNSEVKEDVVEEYVDKLIDKFEVNTAIK
                     LESKHEFKKNILRHLHSSYNRIRFKFPIRNPMLDETKYKHESLYKIIKSIIENEEEFP
                     VFEGIREEEIGFIAAYFGGYLRGSRDNGLRRNKVLLVCPNGLMVSKSLEIQLYKYIPT
                     IEIVGIVSIKQLKEVNVYYDYIITTIDIQNVNNVIVVNPLLTSSDVQLLMNKLISVKE
                     NEKYFNLELIIQAIRKNGVINNEEALKADLLNIIHKIDEGEMYQPMLKELINAERVNI
                     IKSVRDWKEAIKIASKPLLEDNSIEELYIENMIKSVEKYGPYIVLADRFALPHASSKE
                     GVNKLAMSLLIVEDEVDLLGKPVNIFMVLAAVDNTTHIRALASLSEMMYEEENVKLII
                     NGDKSSILELINKQN"
     gene            12701..12988
                     /locus_tag="KPD58_000274"
     CDS             12701..12988
                     /locus_tag="KPD58_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0264065.1"
                     /translation="MKILAVCGFGVGSSMVLKMTIQKALKQLGIECEVENTDINSARG
                     ADCDCIFTSQELLNELKGTCNVDVYPVKKYMDLAEVTEVVQTFLSDKNDKK"
     gene            13121..14380
                     /locus_tag="KPD58_000275"
     CDS             13121..14380
                     /locus_tag="KPD58_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBG0264066.1"
                     /translation="MLDFISNNILRNPSMLIGLIAAFGLVLQKKKASDVIKGSLLAAF
                     GIIIMETGTGMLVSSIAPINGAFQSISGGAVTKGLSDVTFTASYGGIIGLAMFAGLAI
                     HLMIARFTPIKTIFLTGHMLYWFPFIFVASGVEAGLNGGVLIAFAAVLSALYWSIMPW
                     LLRKYVFDVIGDDSFTLGHPSGCLALISGFVAKLVGNKEKSTEDINIPQSLSFFREIS
                     ISGGMAVFLMFIVVGIVIPGAFKDSNEFIGTYAIKQGLSFGAGLIIMLHGVRMLINQI
                     MPAFQGISEKVVPNALPAFDCPILFNYKPNAVLMGFLVAMLTSTAMILICNYFNVFNL
                     LLIPLVITSFFECGAAAVIGESQGGIRGAIIGTAIAAIFMVVLVGISAVIYSGTIQNW
                     ILIFGGNDLSLFGIISRFIGSLFGGIL"
     gene            14383..15123
                     /locus_tag="KPD58_000276"
     CDS             14383..15123
                     /locus_tag="KPD58_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG0264067.1"
                     /translation="MKFKYIDEIRRLIDIIENEEKVNMEKAVQIFVEAIESKNAIFSF
                     GASHAGILTEELFYRAGGCVLINPVFAKSLMLDTAPITHTSKMERLEGYGTALADKTP
                     IKKGDVVIVHSVSGRNPVSIEFAMKAKENKAIVICITNLSYSKSVSSRHSSGKKLYEV
                     CDLVIDNHGQKGDACVEIDGLNQKVSPTSTVIATTVMNSIIAQATQELVNKGLKNPPI
                     FYSANIDGGDELNKKIFDEYKSVIHYEY"
     gene            15446..16756
                     /locus_tag="KPD58_000277"
     CDS             15446..16756
                     /locus_tag="KPD58_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBG0264068.1"
                     /translation="MKKENKMEGIKVGMLKKLCSLAMVAIMTTTLITGCSSGNGKDSK
                     KDGEKEKLVLWMPPVENNMKEVWDPILDKFEEKNNCEVDLQIIPWDNYSEKFATAISA
                     GEGPDVGYMYAEMYPQFIESGAVEDLTNYATKEDKEQSIYIKTSEMMGGMYGMPFQAA
                     NPGVLYYNKDILEKLGEKPPKTWEDFKRICKKATQDTDGDGKIDQWGLAQGWGAKTFG
                     NLNWNWYPYLWQAGGDIFNDDLKSVKFNDKSGLEAANFLKELQAYVPEDSLSKDSNEM
                     IESVFGPGKAAFTIMLSSAATSVFDESFPDLNWGFVTGLEDKKAATFGAVDHLSLMSS
                     AKNKELAYKLIQHMLSVESMTEFHKAIPRAPITKGEPYQGDERFKEMVENDKNVYRPL
                     VVGPHGVEIYEYLWKELQTMISGDKTPKQALDDAAKYSNDLLAQ"
     gene            16854..17771
                     /locus_tag="KPD58_000278"
     CDS             16854..17771
                     /locus_tag="KPD58_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBG0264069.1"
                     /translation="MENLVREFEENSKRKKTKLNIKPYMYILPLGIILVAFYVIPIIM
                     SIYFSFTKYNIISPATFIGLENYKKLFTDETLKVSIINTIKFTVVVVPCQTILSLILA
                     VWITGKGNSKIASFAKGAIFIPVLSSMVLIGMVWRALLNGEGSIIYQVLGAFGIESSK
                     LLGDSKTALPTLMFISMWKNIGYFMVIYISAIMNLPKHCYEVAKVDGATKLQEFIKIT
                     VPLLKPTTIMVVFLGSIWSLQVFDLIYTVTGGGPGISTMSIVMHAFNLNFKNFNSGYA
                     MTVANVLFLLIAVVSILQNKLVRRDNSDF"
     gene            17787..18596
                     /locus_tag="KPD58_000279"
     CDS             17787..18596
                     /locus_tag="KPD58_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBG0264070.1"
                     /translation="MKTARRLLSDGLLLFIACASLVPFIYMLIISLKITYNSYSLDIS
                     FSTVTLQNYIDIFTKKGFAQYFFNTAIVSFSGVLLNLVFSTLAGYSFAKMDFKGSDKL
                     FLFMIMTLIIPSQVTMIPLYIIMKHLGWINSYLALIMPIPTAFGVFIMRQAILGVPKE
                     LLESAKIDGCSDFRILIQIVLPLIKPALITLAIFTFMGAWNEFMWPLIATTKDAMRTL
                     TVGLSTLKTFQITNYGQMMAGATITFLPPFIFYLILQSKFVEGVSLSGIKG"
     gene            18654..20402
                     /locus_tag="KPD58_000280"
     CDS             18654..20402
                     /locus_tag="KPD58_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HBG0264071.1"
                     /translation="MEISYVLKDCTFKHNKYSVVEAETWKDKDLDIVVAKGEKFAFQI
                     MLKATEEFNCTIDKSSAISWKGLGNRVRLALNMPNSLENNFSINILGYVQDDTKAFVN
                     DAILRDKDVLVEQYLPQTFWIEGQVPEDFEENNLDIGIDIFKSFGYEDEEKVCTIDVP
                     VKVRNVVLKPLDESKFFLDLWQHPSCLARMYKVELWSDIHFEIVDNYLKELASLGEKV
                     ATVIVSDYPWAGQSCYKVYKNPSNLYEYNMVSVSKGLDGKIKCNFESMDRYISIADKY
                     KMAKEIDLFGLLGNWCAGEFGNPVEGYKDPIRVRYFDEKDKVFKFINNTNDLKEYIGL
                     VLNHLIECGLWDRVRIIADEPNNPEVVKECIEFINSTVGTHQVKYKSATHDQNFLDRA
                     KDEIDDMSINLKLTIQNYKDIESLKKKINDKGGILTWFVCCFPEKPNSFLSSPFVENR
                     IIGWYTYYFGLDGFLRWDYNLWTEDPWKDSSYKFPIWKAGDMFFVYPGKDLKPVRSVR
                     MENLRFGIQDFELFTMLEKEKGREYIEVELMQELLNKKENAEIKGFGDIELGYSLDNW
                     GYDKVKKYVLELLDRV"
     gene            20664..21686
                     /locus_tag="KPD58_000281"
     CDS             20664..21686
                     /locus_tag="KPD58_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HBG0264072.1"
                     /translation="MFGGINIKRIGIIIMFFLSISFIGFYLVIRDNSDTVKMQRGINI
                     GNALESPKDFPWDVKMSNKFFDDIKDAGFDTVRIPVRFSDYTSDSDNFKIDEEFFKKI
                     DKYVDYALDKDLIVVLDLHHFEEIMKEPRVHKEKFLKIWQQIANRYQKYDKKLVFELL
                     NEPKENLYSQLLNEYIEEAIKIIRKTNPKRTIIVGPYNFYQIDYLNELNIPKDSNIVV
                     SFHYYEPNDFAFQGNIYHKGFEHLSNITWEGTNEQMDYLKKRFDTVENWANKNKVKIF
                     LGEFGITKEAPEASRRAWIKAVREEAEKRNFSWAYWELASGFGIYNQIEGTWDRDILS
                     ALIEKR"
     gene            21691..23997
                     /locus_tag="KPD58_000282"
     CDS             21691..23997
                     /locus_tag="KPD58_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG0264073.1"
                     /translation="MKKNSKKILITLTIITNVIYILWRIFYTVPKEEGKFALICAIIL
                     LFVEIMGMMEMFVHYYGMSNIEYPEKPIISEELYPHVDVFIATYNESVDLVRKTVNGC
                     IHMQYPDKKKVHIYICDDGNREEMCILAEKMGVNYITRTEREGAKAGNLNNAMQHTNS
                     PLIATFDADMIPMHDFLIATVPYFLKNEQAKKDGEKEEYEKVGFVQTPQSFYNPDLFQ
                     FNLHSEGRIPNEQDYFYRDIQLARNRTNSVIYGGSNTVISREALEEVDGFYTYSITED
                     FATGILIQSKGYRCYAIPEVHASGLSPTDLKSLIKQRERWARGCIQTGRRLNILFRRG
                     LGFWQKISYISSITYWYASIKRFVYIMAPILFSVFNVIVVKCTLLQVLVFWLPMYILS
                     SLSLKIFSQNIRNTRWTNIYETIMFQSLMPAVILETFAISKNKFSVTNKSKLEENRMY
                     KFLQGIPYFIYMVLSIIGILKMFVAIFKMSSMTYSVVLFWLIGNLFNLVMATLFISGR
                     QQLRKSERYIAEIDFKLKQNSYVLSSKTIDISENGFAFLLENPEYISPEEEFEVEFRE
                     KSGNEMYIANMKAKIVNVVEVNSKWKYAAYITHIEDSEIDNWMCIVHDRIPTLPMTIS
                     NQLGFFDDLQINVKKRIEKTRTLSRRSPRINMNFQMDIKNTGKLRIVNFNYQYVLLNF
                     ENKSIYPKEIALEINENIVLECDLCEGKIDERGILYKVNNIDSIMQNFFLRDEMMDWI
                     LQNKKILDSKPSEKKEKSIDEFEPMEYI"
     gene            23969..25084
                     /locus_tag="KPD58_000283"
     CDS             23969..25084
                     /locus_tag="KPD58_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264074.1"
                     /translation="MSLNPWNIFRRDEMKTLKVFFCSTKYLILVFIFFINTTFISSAT
                     SDKILDLSFKKIETDLSSKITYEDTGVKIETDSSKSDKERYLYIYQNIKENWSMYNNF
                     YIEIQNKNKSSQRINLSIQSKNMLEFRLKEGSEVFLEGKNIIYSDKIKEGCIEVPGEF
                     EGKIYVNFNSLINEESNVVLDSNMLSNIVSWGITFIPSDEEHNIVIIKKISLLSEEKL
                     RFLNNIKIIGDEEVQIPVLGQSISQYEVLGLKSDSKIKYSLMGKQDNVSISQKGKLTL
                     SNKAKPGQIILQVNVDDKFKIGKKITLTESWSVNKKDKDGVPYTLVYPEQSPTVQDMK
                     KINFMNNIITFVRILFVSLVIICFGIYLYWKKCSKTK"
     gene            25102..26520
                     /locus_tag="KPD58_000284"
     CDS             25102..26520
                     /locus_tag="KPD58_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HBG0264075.1"
                     /translation="MLFSSLIFLFYFLPITLVLYYVFRFNRTIQNMILLAVSLFFYAW
                     GEPKFVVIMIVSIIMNYIFGLLVDRYRESKIKVKVFLVLMCAYNIGVLFIFKYLAFAL
                     RNISELISTELTIPSIVLPIGISFFTFQGMSYVIDVYRRHGEVQKNPFYVGLYIAFFP
                     QLIAGPIVRYESVAEQILNRKETWNKFSIGTCRFITGLGKKVLISNNMAIVADYIYTM
                     NSQGEIAASLAWLGSIAYTLQIFFDFSAYSDMAIGLGLMFGFKFEENFNYPYISKSIS
                     EFWRRWHISLGMWFKSYIYFPLGGSRVANKDIMIRNMFIVWLFTGIWHGAEWTFVIWG
                     ILNFVFLIIERFIMFEKIENHNFIKHIYALLVVNFGWVLFRSPNLKEAYNYFKAMFGG
                     NGAIWSDYTYMFLKEYWVFFIFAFIFSVPIAKKINKFVVEQAKYSMIFNVFYPISAIL
                     LFFISVTYLVTGSYNPFIYFNF"
     gene            26535..27743
                     /locus_tag="KPD58_000285"
     CDS             26535..27743
                     /locus_tag="KPD58_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264076.1"
                     /translation="MNRFFKVRLITAIMFLIILFAFSIFNVCYSGKDTIQEIKNSIAE
                     KSSLKDTISSIDNTVNDKVIYKNTFIETYGYLQKLMGKNEFSKFSVVKDTSGKMHYTY
                     FAKAPNPVGVIADRVRKFSDEMEKQNTKLVYLMTPDKYIKGVTKFPKGIPYSYSNETA
                     DNFLNQLKKYNVDYIDFRENILKSGIPKDDLFFKTDHHWKVETSFWAFGELVEQLNKK
                     YNMNLDENHYYRDKDNYNSIVYPKSFLGSMGRKTGILYGGIDDFTLIYPKFKTNYTYY
                     TDSKSQKFELKGRFEESLILSYPFNADLDLMDGQSDKYFTYLLGNRPLVKIKNIENPD
                     GLKVLFVKDSLIVPTAAFFSSVCSSIDMIDPRYYDGDILEYAKSHDYDFVFISVYPQN
                     LTKEFFPFCE"
     gene            complement(27817..29205)
                     /locus_tag="KPD58_000286"
     CDS             complement(27817..29205)
                     /locus_tag="KPD58_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HBG0264077.1"
                     /translation="MESSFLSQFLMITDFKTIFFIILLLGTFFIVRQFEKKKIKFSLR
                     TIYATVLGLILGIIIQAFAGFPNDTTQVIWLQEVSKWYSLFGSGFMDLLKMLVVPLVF
                     LSIIKVIINMKDNNLGTLTFKSLGMLLATTAIAAIVGIIVANIMKLGVGANMPTLVEN
                     QELKEINSLVDTLRGLLPSNPVMAMANGNIVATIIFATFIATSIKRLSKKHFETIKPF
                     IGFIEASYKIIVSVSMTVIKFMPYAVVALLANTITSQGISSIVSVLQFILALYISVAI
                     LFIIHLIFITLNGLNPITYIKNSTEALLLAFTSRSSLGTLPVTIESLVQNHGVDEGVS
                     SFTASLGANMGMNGCAGIYPALMAVTLANMAGVNMDMSFYVMLLIVITISSFGIAGLP
                     GTATMAVSVVISGVGLGSYFPLVGGILAIDPILDMGRTMINVNGAMVSSITVGKSLGK
                     IDKEVFSKKNIS"
     gene            29546..30880
                     /locus_tag="KPD58_000287"
     CDS             29546..30880
                     /locus_tag="KPD58_000287"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HBG0264078.1"
                     /translation="MRVIIIGGVAAGMSAAAKLKRIKPEYEVVVYEKTEIVSFGACGL
                     PYFVGGFFNDADELLARTPEKLREAGIDLNIFREVVEVDSESKKIKVKNIKTDEIYED
                     YYDKLMIATGARSIMPPIKNIKLKNVSTLKSLYDGEYLKKLLSNEDNKRVTIIGAGFI
                     GLEAVEACKKLGKDVDVIQLEDRILPQVFDKEMTDVLEEEINRHNVNLHLDEMVVELC
                     GEDKVEKVITNKGEIDTDVVIIATGVRPNTEFLSNTNIKMLKNGAIVVDEYGRTSVED
                     IYSAGDCATIKNIVSNENVYVPLATGANKLGRIVGENLAGREISYQGSLSSSCIKIMD
                     MEAASTGITERQAKDLGINVKSKFISDYNQTHYYPGRNKIYVKLIYDADTKVILGGQV
                     AGFKDAVQRANVLAAAIFGKMTTNQLGMLDLCYAPPFARTWDVLNVAGNVSK"
     gene            complement(31022..31939)
                     /locus_tag="KPD58_000288"
     CDS             complement(31022..31939)
                     /locus_tag="KPD58_000288"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HBG0264079.1"
                     /translation="MIDLTLLGCGGNVPMPNRFLSSVFINYKGRKILIDCGEGTQVSM
                     KLKKCGFKDIDLICITHLHGDHIFGLLGLLSTIGNSGRTSDLTIVGPVGIVDCIRSMR
                     NLVEYVPYTLKIIENPQGNFSLDNKVLRNLEISTISLEHSIECLGYSFNFRRNPKFDI
                     DKATKNEVPKILWNKLQEGQNIVLDSKQYTPDMVLGELRKGVKISLTTDTRPIESIPD
                     FIKDSDLFICEAMYGDDLDISKAVRNKHMTFREAANLAKLGNVKQLLLTHFSPSLDIP
                     SMYLENATNVFENTILGEDRLSLRLNFDE"
     gene            32147..33031
                     /locus_tag="KPD58_000289"
     CDS             32147..33031
                     /locus_tag="KPD58_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HBG0264080.1"
                     /translation="MKVLKKITILSLITLTLVGCSNGEDKKVVEGSKKIQNSKKDEQP
                     IIVHKDANILKDRYEPPKGFKRVDVEKGSFESFLRNTKLKNYGEKVKYYDGRTKESKG
                     IYDSVFDVDIGNRDLHQCADAIMLMRAEYLYQNKLYDEISFDFVDGFKAKYSKWAQGY
                     RISVKDSGSSWYKATEYSTSYESFRKFMDIVFAYSGTLSLEKELEPVKVEDMKIGDVF
                     IVGGSPGHASIIMDMAENEKTGEKVFMIAQSYMPAQQTQLLINRKNPNLSPWYSLDFE
                     GDLTIPQWTFKRDQLKRF"
     gene            33244..35085
                     /locus_tag="KPD58_000290"
     CDS             33244..35085
                     /locus_tag="KPD58_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HBG0264081.1"
                     /translation="MKIRRYLILVIIILITTSQSFISYGLERFEEITIFHTNDIHGRY
                     AEGDDHIQIGNLATLKKETPNSILVDAGDCLHGLPIVNMDRGKSAIELIKCAGYDYIT
                     PGNHDFNYGKDRLFELSKSANLGENTLKFLSSNIFENGKKVFDSNDIKEIDGIKIGFF
                     GLSTQETKSKTGFKNVEGLEFKNPIESATEQVNELKGKGANVIVAICHIGTNSLSKPN
                     SIDIANEVQGIDLIIDGHSHTKFENGKQVGDTLIVSAGQYLENIGEIKLTLDTNDVEN
                     IKIENKSVRLIGKEEALKYKLDSAVSKKVDEIKEQQEKILDKVIGVTTNTLDGSYENV
                     RTKETNLGNLISDILLDKTKADISLFNGGNIRDTIEKGNITRRDIVDVFPFNNTIVTK
                     ELTGAQIKDVLEHGVKLYPEKSSAFLQVGGISYYFNPKQKEGEKITNIQKEGKPLDLN
                     KKYVVATNDYIASGGDEFPCFSKEPVLKEFGNLESAVIEYIEYKTEISKNVDGRIGIK
                     SKDELISKNHESDLILSENEKNINDNEENLSNNIKSSDSDKNKSLDKTSARESSNLGI
                     SKREAKEKSPKTGDLGFSNSIIIFIVSSTLICLLNFNQKELKDKKSK"
     gene            35390..36097
                     /locus_tag="KPD58_000291"
     CDS             35390..36097
                     /locus_tag="KPD58_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0264082.1"
                     /translation="MNNILLLEDDKSLNRGISFKLKKEGYNVFSAFGIEEAKNIFAKE
                     EICLIISDIGLPDGSGFDFCEEIRKKSDVYIIMLTALDEEVDIVTGYDLGADDYITKP
                     FSLMVLISKVNALMKRVNTVKNYTLLVCDDLFFYYIENKLIIRTDDKEEEIILSKTET
                     KLLKYLMENSMQTLTKEQLLESLWDSSGNFVDDNTIAVNIRRLRQKVEKNPSEPKYIK
                     TVRGVGYMWGERSIKKC"
     gene            36091..37401
                     /locus_tag="KPD58_000292"
     CDS             36091..37401
                     /locus_tag="KPD58_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0264083.1"
                     /translation="MLKNMNLKDGLSVKDDYIMRRVIVYSVLSVLIVTLVFILFEYSR
                     LHKLYEHQIKNTQNIAGILIEKYPNDEIDIVNSIYNSDYSKVSTGEKAFKKFGYGLEN
                     KMSNDKNFSLYLKSFFKESFFIFLMMFIVVIGIIYYFIRYINKRLSKIYFIVDKMSQE
                     NYLKDTTESEETGYLVEYNEYFKEGIFSKINNCLYELNRSLKIKFIKLEKEKESVKSL
                     VTDISHQLKTPLASLKLYNTLLIEEELEEDEANEFLLTNKASINKLENLINSLVNISR
                     LEISMINIKKEDNDIKNTILNAVKSVTPKAKSKSITLNLNEFNSRIIPHDKKWTEESI
                     FNVLDNAVKYTQVNGEINISVEETANFFKIIIEDNGIGIEKNEFNNIFKRFYRGSADE
                     IESVEGSGVGLYLSRKILEEQGGNIIVSSKVGIGSKFSLFLTTM"
     gene            37518..38201
                     /locus_tag="KPD58_000293"
     CDS             37518..38201
                     /locus_tag="KPD58_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0264084.1"
                     /translation="MKILYTENLSKHYGKGESLVRALDNVDLEINEGEFVAIIGKSGS
                     GKSTLLHMIGGLDIPTSGKVYIDNKNIFTLKEEELAVFRRRKIGFIFQSYNLIPSLNV
                     WENVVLPIGLDGREVDESFIKELLKSLGLENKHDVLPNTLSGGQQQRVAIARALATRP
                     AIILADEPTGNLDSKTSDEVMSILKSMSKKYSQTLVMITHDDSIAQMADRVIFIEDGR
                     VSKVGDKND"
     gene            38194..40581
                     /locus_tag="KPD58_000294"
     CDS             38194..40581
                     /locus_tag="KPD58_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG0264085.1"
                     /translation="MINTTKIAKSNLKQNKSKSILIIITIILSTLMLSSIGIYIVDAG
                     AYQKENTIKYSGNYQGILANVDEKQADILSNHADVELTGEMNGVGVEKLEDDSNISLA
                     YMNEDALKLNSFEFIKGKLPSKENEIVLDSGALKALGYKNKDLGEKIKISYDDYKSDK
                     KIEKEFIISGILKTSEISEAGKYYYAIISESYMRNTRDMSQEDFNIYVKLNDKSNLSI
                     EQAKSKLNKIANDVGLDTINVAVNENYINALKPDMETIMGGVFVGLVIVLSSILVIYN
                     IFYISIVTKVQEFGKLRAIGATKKQVKNIVFKEGFILAGIAIPIGIILGYVLANIIIK
                     SFMDIDVKSSRLPVILSVVVISFISVVLSLLKPMKVASKVSIVDAVRYTGNKISNKSK
                     RKGYKNINLKRLSHANLERNKKRTYMTLASLILSGTIFITVSTALESFDAEKMAREHF
                     PYDIEVRLSGYEMNSDKNPKDNLNILQMDNPLSKDFFNQIKNMEGIKRIESARSVKIG
                     MEDYDVEFKYDLLQSINENDVKSLNKNIIDGKINLERLQTGDEIVITHVDTAKEMGVK
                     AGDKIRLTLYDGDKKIKKEFKVQAIAMGVPSFGIGKDFIDRTLKYDSTSSLGIYTEEG
                     KYQEIKDSIKKIAKNNGFLETDFIDSRIESNKATISFIKIMGYTLTGIIGVIGFMNLV
                     NTMITSIVTRKKELGMLQAIGLTNKQLVKMLNSEAMYYTSGMMIGSILFGGILGYIAV
                     IFLKKTGLSYATYSLPIVPILLMIVCILIAQFITTYLIGRSFNKESLIDRVRYSE"
     gene            40861..42003
                     /locus_tag="KPD58_000295"
     CDS             40861..42003
                     /locus_tag="KPD58_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBG0264086.1"
                     /translation="MNKKINYAPGPTETRENVRLARAEKTTNPDIDIDFVEFYKKTCE
                     KFGNIIGTKNDVYILSGEGILGLEAACASLTEKGDRVLVIDNGIYGEGFKDFVTMYGG
                     EYVLFSSEYTKSIDIDELKAFLDEDSNFKYATVVHCDTPTGVLNDVSKICPLLKEYGI
                     LTVVDSVAGMVGERLSVDESKIDIILGGSQKAISAPAGLTIVGISQDAKNCIKNRKTD
                     VIGFYCNLNIWEGYYEKKYFPYTMPISDIMGLDKALDNILEEGIENVLNRHEKIAYSV
                     RKSIEEYGLELFLEEGYSNTVTAIKIPESIGALELTDYMLKNYNTLVATSLNQYMDTI
                     LRIGHMGENANLNKIEHVLNVLDKSLSALGFKGNGNLLNLFNKYYF"
     gene            42022..42546
                     /locus_tag="KPD58_000296"
     CDS             42022..42546
                     /locus_tag="KPD58_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG0264087.1"
                     /translation="MEVTAKQKFTTKDMVETSLLIALVFIATKFINIRLPISINGGLV
                     HLGTAMLFISAIVFGSKKGALSGAIGMSLFDLISGWTLWAPFTFIDRGVMGYLLGKIA
                     WANGNNGNNFLINVIGICVSSIWMLFGYYVTEVILYGNFIVPLTSIPGNLMQVLIGLI
                     IALPISKVLKKCIK"
     gene            42646..43464
                     /locus_tag="KPD58_000297"
     CDS             42646..43464
                     /locus_tag="KPD58_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG0264088.1"
                     /translation="MLSVTRYSIKNVRLKPWVKFIWYFETKSNILLNNKLLPTDSIDI
                     ILNLSDVMEYKIENQDYIASNIHFNGIRDKHGFIIQHGNIRVIGISFYPFGLYPFLKI
                     PISEFNRQIVDLEAVSKLFAKKLEESLNPTKAIEKVVLCLEEVLLSILEEDLISNKYV
                     KLLNSFIYVNRYSNIKAFCDDTNINIKTLERICLKYTGYTPKILKRIYRFKMASNQLI
                     YNYKDDELFDFIYENEYYDQAHFIKEFKKFSGTSPIKFSGENKTIKENIKYSYL"
     gene            43542..43853
                     /locus_tag="KPD58_000298"
     CDS             43542..43853
                     /locus_tag="KPD58_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBG0264089.1"
                     /translation="MKIITEIVEFNVEENLSKEEFIEIVNDLELKFHSIQSGFIDTEL
                     LYDEKSQKWIMIQHWESIEDLKSVSSKMFKDSSTEKFRKALKNQTVKMNIIPQIKSWK
                     L"
     gene            complement(44030..44713)
                     /locus_tag="KPD58_000299"
     CDS             complement(44030..44713)
                     /locus_tag="KPD58_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264090.1"
                     /translation="MQKEVAMNKLIKDCVNFFNDCGFSYSICGGYALELFANMHIRPH
                     SDIDIWISDTDKEKSIQYMLNQGWDIYEPLGNYLLRPIIDLNKQHIEQICVFAIKPDC
                     SFIELKKVEDDNYKMNIQCDEQLQFDFIELIYNPQKDGKFLFSQNQRIIRDMDKARLI
                     TSDNIPYMAPEVVLFCKSIYIDRLGYQKDFDVTVPLLSQEQRDWLVNALITAYPDGHA
                     WIDQLANQY"
     gene            complement(44806..45447)
                     /locus_tag="KPD58_000300"
     CDS             complement(44806..45447)
                     /locus_tag="KPD58_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0264091.1"
                     /translation="MGRKKEPDNTIQKILDISHKLFLEKGYEQTTIQDIVDNLDGLSR
                     GAIYHHFKSKDEIIKAITSRLYNDGFYGYEVKNDEGKTALEKLQYRLLHAYEQHNKQD
                     IFLMSSSLLRNPKYLATQFDEIINDIVPWIKALIEEGNQDNSMEVKNPEIAAQMFGFL
                     LNTWCIPTVYPGNPQQMIERFTLFGEQAAFMGISFMNDKIMCEYQKFVNKFSK"
     gene            complement(45621..47624)
                     /locus_tag="KPD58_000301"
     CDS             complement(45621..47624)
                     /locus_tag="KPD58_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HBG0264092.1"
                     /translation="MKSQKRFKILDIDVYLQPFESDIKKRMKHYEDMKSKTLKNYKDF
                     SSFANAHLYYGFHQTNDGFFYREWAPNADSLSLIGDFNNWNRKSHPLRKIGSGNWEIF
                     IPGQNSLPHKSEVKVQVTANGKTFDRIPLYIKRVIQKDFGGFNGQIWQPKTPFIWTDN
                     DFDLKNITSPLIYECHIGMSTESESIGTYNEFTEKILPKIKKAGYNTIQLMAIMEHPY
                     YASFGYQVSNFYAISSRFGTPEDLKNLINTAHSMGIAVLLDLVHSHAVKNTLEGINEF
                     DGSEHQFFHSGSKGNHPAWGTKLFNYGKPEVIHFLLSNIKFWLNEYHFDGFRFDGVTS
                     MLYHNHGLGVSFDSYEKYFSMNTDIEAITYLQFANELMKEIKPNSISIAEDMSGMPGM
                     CIPIKDGGIGFDYRLAMGVPDFWIKTISNLSDEDWDLGKMWYELTTRRPGEKNIGYCE
                     SHDQALVGDKTIIFWLADKEMYWNMEINSNNHVINRAISLIKLIKLITFSLAGEGYLN
                     FMGNEFGHPEWIDFPREGNNWSYKYARRQWSLSEDNNLKYKQLLNFDKSMLELTKYSD
                     IFIQNSHELIHMDNDKKILVYSKGKYLFIFNFHPNLIQSVDISKFQNTNYKTILNTDM
                     VEFGGNTKKENLRDYDVYFSSTPPSSKMPIASRTAIVLLDNGI"
BASE COUNT        18563 a         5213 c         8106 g        15798 t
ORIGIN      
        1 tttagaagaa attctataag gggggatatt tatgcacaag agattgctca cgttgtttaa
       61 attattaaat gaaagtgatg ataaaattac ttgtaaaact ttaagtaatc atttaaaagt
      121 gagtgagaga actataagaa atgatattac ttctataaat ggaactttag agaaaaatgg
      181 tgctattatt aaaataaaaa aaggagaagg atattatata gatattctta atttagcatt
      241 ataccaacag tatctagcat taatatctga tgatattatg gattcaagtg aaataccaga
      301 ttctcctata gaaagaaatc aatatatttt aaaatatatt ctctataaca atacatatat
      361 aaaactagaa gatttagcta atagcttgta tgtgagtaaa tttacaatac taaatgacat
      421 aaaaagaata aagcctattt tgtcaaagta taatttaata ttagtttcta agccatatta
      481 tggagttaaa gttgaaggta aggaaataga tattcgaaga tgtatttcta ataatatgat
      541 aaataggaat tttgaaaact atataattgg aattacagat agagaaatag aattatttaa
      601 taatgtagat ttaatagagc ttcaaagggt agtgttaagt gaaattaata aatttaatat
      661 caatttccta gattttaatc taaaaaactt tattatccat ttagctatta ctgtaagtag
      721 aatacttgat ggttactgct tagataatgt tttagatgtt gttttaacag attttcaatc
      781 gaatacaact gttgaaaata tatttaatta tattgagagt aaatatacta taattatttc
      841 aaaggcagac agagtttatc tatataatca ttttataact aaaagttctc ttttggataa
      901 cgtttccaat agggtagata ctaaaattat agagtatgtt gaagaaattt tagaagtaat
      961 caacaatcaa tatacttttg accttagaaa tgatagtgta ttatttgatg atttagtact
     1021 gcactttaaa tcaattctca attctaaatc ttataattta aataaggtga atccccttat
     1081 aaatacaata aagagtaatt atcctttagc atttgaaata actttaaatg ctatagaaaa
     1141 agtttttaaa aatagtattt actctttaac agaagatgaa attggatatg tatccctgca
     1201 tataggtgct ggaatagaga gattttttca aagtaatatt aagtgtaaaa acgtagtatt
     1261 agtatgtggt tcagggtatg gttcaagtag gttattagag gttcaattaa acaaagtttt
     1321 tcatgataaa ataaatatac ttcaatgtct atcatttaat caatttttag ctagtgaatt
     1381 gtcagatgta gatataataa tatcgacaat tcctttaaat catgatagta ttcctattgt
     1441 attagtggat ttaaaactat taaaaaaaga tatagagaat atatctaagt caatcactaa
     1501 taattcccat atttatagta atcttttgga caatttcttt gataaaaatt tatttattgt
     1561 taatcctaaa atcaaagata aagatgagct tataaagttg atgtgcaaca aattaactca
     1621 aagtgaaatt gtatttccat catttgctga atctgtattt tacagagaaa gtttatcttc
     1681 taccaatata gatgattttt tagctatacc tcatcctatg gaattaagct ctataagaac
     1741 taagatttgt atttccatat tgaatgaacc aatatattgg agtgaaaatt ctactgttaa
     1801 attaatcatg atgttggcta taaataaaga tgattatata aaaataaact ccatttatga
     1861 tatcctttta aagataattc atgacaatga tattagagat agtatatcta attgtaatga
     1921 ttttgataat tttctttcca taatcaagtc tatagtatag atttttatat tacataagtt
     1981 ttaaaaactt taataaaata ttttttgttt ttagcataaa tagtataagt aagttttgta
     2041 taatttgtta ttattttgta ttgattataa ttattataca ataaagattc tgtctcaaaa
     2101 tagagagaga aacctgtttt tcattgataa aatgataaaa atatatctga tttcatacaa
     2161 aaaagagtgt ctcatgaact aaaaagttca ttttgagaca ccacctttat tattttatat
     2221 tatttatctc ttgtatatat tactatagca tcccttaaaa acttagctaa tccaggttgt
     2281 tctctatcat aatatgatgt aaaacgttca tcatatacat acatctgtgc tagagaggaa
     2341 tgagcttctt tagagtatgt gttccaagtg taacacaacc atttatgatg taattttgca
     2401 acttcctgag ccagttcact agaggcatca ccagttttaa aagctttttt aagtttacta
     2461 attatttcga tatttaggtt ttgccaattt tcataatctt gcttagacat atttttaagt
     2521 tttttatttg atgtatctat aatatcttta ccatatttct ctctaatttc agttccataa
     2581 tttttttcat tttcatcaat catcttttct ttaaaacctt caaatttttc tatatctgtc
     2641 atagtgtatt ttccctcctt cagagctata gttttagtta cattttctat tagtaaatca
     2701 atttgctttc ttttggctag taatttatta tggtgttctt ttaacgcact taaactatcg
     2761 aagtttggtg aatttataat gttttttata ttttctaagt taattcctaa ttctctataa
     2821 aaaagtattt gttgaagttt attaacttca ttttgaccat atatacgata acctgaagaa
     2881 tttattttaa gtggcttaag aagacctatt tcatcataat atctaagtgt tctagtactt
     2941 ataccagcta gtttacttaa tttttgaact gtatattcca taagtagtac ctcctataaa
     3001 ttaaatatac acttttacgc aacgtaaatg tcaatacatt aaaatataaa aatttatatc
     3061 ttttaataaa aatataaaaa aatatcttta ctggatattt tacacacaac tgtcaatact
     3121 cactagtgaa aattaaaaaa taaaatttat agactatctt tactaaaaaa tagaaggtct
     3181 atagtctagg aggaactgta tgaatttaaa aagaagcaat gaaaaacgag aagagtacag
     3241 aagaatgtat tcagataaag aaagtttttt aagtttgatt caaaatttta caagtgtctc
     3301 aatagcaaaa gaattaactc ttaaaaatat agagttagaa acatctttta tatgtgaata
     3361 taaaggaaaa gaagttgata taatttataa ggtattttct aaaagcggca aagtatcaca
     3421 ttatatagtg ttagaatttc aaacagaaat ggatactgaa attgtcccaa gattgaaatc
     3481 gtatagagaa caaatttgga aaagttttat tatgaaaaaa agccttgaag aaatagaaga
     3541 taaaaatttt aaacttccta aagttatacc agttgtttta tatagtggac ctgaaaggtt
     3601 aagtagaaaa aaaggaattt tggatattgt agaatctata tcggagttca gtaataattc
     3661 tagtatgaaa aattaaaatt acataatgtg tttattggtc taaaatggaa aactgtcgca
     3721 taaacttgaa tatgaaaata tttatagata caggttctat gtggaggtgt tccatgagaa
     3781 ataacaagtc taaggtattg ctaggaatac tttgtctagc aacaggaatg gctgtaattc
     3841 tgtcaatgtt gcttcctggc tggatttggt cagcacttac cgcattaata cttattggat
     3901 gtggagcatt attatttttt tgttaaaata gttttaacta taaagtgtgg ggtgagtatt
     3961 gatgaaagta gtaacaataa aattaccaaa atggatttca agtataatcc taaaatttat
     4021 gagaaagagt aaaaatgatt aaattaaaaa gcctacttac ggaagtaggc ttttaaacgt
     4081 ggttactaag ctctttcaat tttaccagaa cgtaaacatc tagtacaaac ttttactctc
     4141 ttaggagctc catctataat cgctcttacg cttcttaagt tagctgacca tgttctttta
     4201 ttatgtttat ttgagtgtga tacttggttt ccagaaacct tacctttacc acatacgcta
     4261 catacttttg ccatctacgc acacctcctt aaaatgataa ctatttaaag actaaaaaca
     4321 tatttatatt atcataaata acaaaaattt gcaatagaaa tatatatttg ccttacacat
     4381 attgaagaat ttcttattat catataaaat tatatatata attttatata aagtaaatat
     4441 atttggaaaa taattattat ttttctttga atatatatac tatagaagaa attttatcca
     4501 aaaacttttc taggtttaaa ttataccata tttatagtat gatatgatta taattttata
     4561 taataaataa gtaaaaatag taaaaaataa attaagaaat gtttttaata aaaataagct
     4621 taagggggtc aaccatgagt gcaaaggtaa cgaatcaata tggaaatata gaaatagata
     4681 atcaagtaat agctcaagtc gtatatagag ctgcaatgga aagttatggc ttagtaggtt
     4741 tagcatataa gtctaaagga atagtagagc tattaaaagg cgagaacgca actaaaggtg
     4801 taagagttga ggaagtagaa gaagatgcca tagcaatcga actttatgtt ataatacaat
     4861 atggtacaaa tatatctact gttgccaata atataataga tagagtaaaa tatgtagttg
     4921 aaaaaatgac tggcgtaaga gttactagaa ttgatattaa tgtacaagga ataagagtta
     4981 agtaatttca taggaggaaa caaatgattc aatatataga tggtaaaagg ttaagggaga
     5041 tgtttatttc aggtgcaaac aatctccaaa acaataaaga gttagttgac aagttaaatg
     5101 tattccctgt accagatgga gatacaggaa ctaatatgtc attaacaata tcttatgcct
     5161 taaaagaatt agcaaaagtt gaaaatgata atatatcaga tatcggaaaa gcattatcta
     5221 aaggttcttt aatgggagct agaggaaact caggagttat actttcacaa ataatcagag
     5281 gtatagctaa atctatagaa ggaaaaagta aattatcaac agaagattta gcaaaagctt
     5341 ttaaaaatgg ttcagacaca gcatataaag ctgtaataaa accaattgaa ggaactatac
     5401 ttactgtagt tagagaaagt ggagagtttg caataaaaac tgcgaaaaaa gagaaagatg
     5461 tagtaaagtt tttaagtatg cttgtgaagg aatcaaattc ttcattagaa agaactccag
     5521 accttctaaa gaatctaaaa gaagctggtg tggttgactc tggtggaaag ggtcttgtac
     5581 ttatatatga aggaatgtta gcttctataa aaggcgataa catagaaata aaaaatgttg
     5641 acctagatac taatatttca acaagtatgg attttgcaaa gagtactact agcacagatg
     5701 atataaaata ttgctattgt acagaattta ttttagaaag ttctaaggta gaagatacta
     5761 aaataagaga cataatgatg gcttatggtg atagcctagc agtagttgga gatgatggag
     5821 tcataaaggt acatgtacac actaatgacc caggaaatgt acttcaagaa gctctaaagt
     5881 acggtcaatt attaactata aaaatagaaa atatgaagtt acaacatgaa aatacattac
     5941 tggatgtaga agaaaagaaa gaaaatgata gtgaaccttt agaagaagaa aaagagtttg
     6001 ggtttatagc tacttctatg ggtgaaggtt tggcaaatat atttaaagat ttcggagttg
     6061 accatataat agaaggtggt caaactatga atccaagtac tgaagatttt atgaatgcta
     6121 taaagcatat aaatgctaaa aatatattta ttttcccaaa taacagcaat ataataatgg
     6181 ctgctaatca ggcaaaagaa ttgagtgata aaaatataat agttatacct acaaaaaata
     6241 cgcctcaagg atttgctgca ttagtaactt ttaatggaga attaagcgaa gatgaaaata
     6301 aagaagctat gatgaatgct ttaaactcag ttaaatcagg tcaagtaact tttgctgtta
     6361 gggatacagt gatgaatgaa attgatgtta aagaaggtaa tataattgga attgcagaag
     6421 gaaatttatt atcagcagga gattatgtgg atgaagtaac atcaaacctt attgaaaagt
     6481 tggttgatga agatactgct ataataacat tattctttgg tgaagatgtt acagaatctc
     6541 aagcaaatga actacgtact tctttagaag aaaagtttga agatgtagat gtagagttat
     6601 attatggagg acaacctctt tattattact taatttcagt tgaataatga ttataagaac
     6661 ctgtatgacc aagtagcttt tataaaattg gttgtacagg atttttgtgt agtaggtgat
     6721 atatttgttg gatttatata aagatgttca atatgttaag ggaataggac ctaaaaaagc
     6781 agataaatta aataaactgg gtatatttac actaaaagac ctattgtatt attttccaag
     6841 acaatttgaa gatagaaata atctaaagaa aatagcacaa ttagaagatg gtgagaaagt
     6901 tacgataaaa gcagtaatat caagtataaa tacatttagc ccaaaagaag gtatgacttt
     6961 aacaaagatt gatgtaaaag atgagacagg ctcggctaaa ttagtgtttt ttaataagag
     7021 ttatataaaa aatacttttc gtccaggaga ttcaatatta gtttttggga aggttaagaa
     7081 aaaatttaat aacttagaac ttacatcatg tgaattagaa tatcttacta attcacctaa
     7141 gaatacatgt agatttatgc ctgtgtacca gcttacatac ggtgttacaa ataaagaaat
     7201 tatgagtata attagaacag tacttgaaga taaagaatta attatacaag aatatatgcc
     7261 acaaagaatt atagaaaaat ataggctatg tagtatagat tttgcagtta gaaacataca
     7321 ttcaccaagt agtaaggaat cattaaaaat agctttatat agaattgtat ttgaagaatt
     7381 acttatacta caactagggt tgtttgtatt taaaagtggc agaaataaag aagatggaat
     7441 aaagtttgaa acaagtaaag acttaaaaaa aataataagt gctttaccat ttaaactgac
     7501 taaagctcaa aacagggcat tagatgaaat tatacaagat atgaatttag agaaaataat
     7561 gaatagattg gtacaaggtg atgtagggag tggtaaaaca gtagtagctc tcttagcact
     7621 tgcaaattgt gttttaaatg gttatcaggg cgctttgatg gcacctacag agatattagc
     7681 agggcaacat tatatttcac ttactgagtc tctaaaagat tttggaataa atgtaggtct
     7741 attaataggt agtcttacaa aaaaacaaaa ggatacagta ttggaacaaa taaagaataa
     7801 tgagattgat atattaatag gtacacatgc actaattgaa gataaggttg aatttaataa
     7861 tattggttta gttataacag atgaacaaca tagatttggt gttatgcaaa ggagcaaact
     7921 ttcattgaaa ggagcaaatc ctgatatatt agtaatgaca gcaacaccaa taccaagaac
     7981 tttagcactt atactctatg gagatttaga tatttctatt atagatgaac ttcctccagg
     8041 aagacagcca atagaaacaa ttgctattga aaaaagtaag cgagatagag catataataa
     8101 tcttgtaaga agagaggttg aatcaggaag acaagtgtac attgtatgtc ccttggtaga
     8161 agaaagtgaa gccatagaag ccaaatcagc agttgaactt gttgaggagt taagagcgga
     8221 atattttcac gacttaaggc ttgggttgct tcatggtaaa atgaagtcaa gtgaaaaaga
     8281 tgaagtaatg agacttttta aagataaaga aatagatatt ttagtttcaa caacagtaat
     8341 agaagttggt gtcaatgtgc caaatgcaac attaatgata atagaaaatg cagaaagatt
     8401 tggacttgca caattacatc agcttagagg gcgagttgga agaggtagtc ataaatcgta
     8461 ttgtgtacta atttatgatt ctaaaacaga tgtatgtaga caaagaatgg ctataatgga
     8521 agagaccaat gatggtttta agatttcaga gaaggattta gaaataagag gacctgggga
     8581 attttttggg acaaggcagc atggtcttcc agaacttaag gtagcaaact tatttaagca
     8641 tataaaaata ctgaagttag cacagcaaga agctcgatat atattgggtg aagataataa
     8701 tctacaatta aaagaaaaca tggcactcaa aaaagaaatt atagataaat ttaaagatac
     8761 attgaaagaa atttcattaa attaatttta aattgtgtta aaatattact aatgtaataa
     8821 tgtgctgaag ggagattgtc atattgagag taatttcagg aaaagcgaga ggattaaaac
     8881 taaatactcc aaaaaatgaa gatgtaaggc caactacaga tagagtaaaa gagtctctat
     8941 ttaatatgat aaattcatat ataatggaaa gtgaagttct ggatttattt gctgggactg
     9001 gttctttagg aatagagtgc ttatctaggg gtgccaagtc atgtactttt gtagatatta
     9061 gtaaggaaag catagatatt gttaagtcca atataaaaaa ggcaagagtt gaaagtgaaa
     9121 gtattatttt aaaccttgat tttaagactg caatagataa acttaaatta caaaatagta
     9181 aatttgacat aatttttatg gaccctccgt attataaaaa tatgtttata gaagctattg
     9241 aaaaaattga taattctaat ctattgaacg aagatggaat aatagttgta gaacatgata
     9301 cgaatgattt atttccagat aagatatgta aattggagaa aactagagat aagaaatatg
     9361 gaaatactac attaactttt tataagatgg aggcataata tggaaaataa acctagaaaa
     9421 gctatatttg caggaagttt tgacccaatt actaatggac atttagatat tatttgcagg
     9481 gcttcgaagt tatttgacga attacaaata ggagtattaa ataatcctaa taaaaagggt
     9541 ctatttagct ttgatgaaag agtaaaactt atagaaaaaa gtacaagcca tttaaataat
     9601 ataaaagttg tgacttttga tggtttatta ataaattact gtcaggaaaa tgggattgga
     9661 gcattagtta gaggtgtgag aagtggtgct gatgttgatt atgaacttca gatggcacat
     9721 atgaatagag agcttaatcc agatatagag actataattt taccaagttg tacaaaatat
     9781 tcatttataa gttcatcttt aataaaagag gttttgctct ttgatgctga tataaaaaat
     9841 ttggttccaa agattgtttt ggaagaatta aagaaaaaaa ctagtggggg taattgatat
     9901 atgaagatag atttagaaat gattgaacta tttgagcagt tagaggagat atttagaagt
     9961 gcttctacaa tccctttctc acataaatgc acagtggata aagatgaggt tttagcaata
    10021 attaatgata taagaacttt aataccagaa gaagtaacac aggcagtatg gattaataaa
    10081 gaaagaaata aaataataag tcaagctaag caagatgcta caaatatagt tgaacaagcg
    10141 aaaaaagaag ctgagagaat acaacaagaa tatcaagata atatagagga attaaagaaa
    10201 aattctgaag atgtagtaaa ggcatatgta gaatccagtg aacctgttgt tcaagcagac
    10261 caaagagcaa aagatatagt tgatagagct gagagaatag ccaatgagat aagaattggg
    10321 tcaattgaat atgcagagga tgtgctttct agtgttgagt ataatttaag agaaatatta
    10381 gaagaagtaa aaagagataa agctgaactt agacctaaaa aataaatttt aaataaataa
    10441 aaatggagct atcacacaaa aaattccaat ttatgtgtga tagctctctt ttaatatagc
    10501 aattttttgt tatataattg tgctttttag attacttata tcttattttg atattataat
    10561 gtagtttaat cttttatttg ttgaaatcaa cttatttatt tgtataaata atataggtta
    10621 ttttcaaatt tttttaataa ttttaccact gtcttctttt agattagagt tagactgtgc
    10681 aaaattataa aataatacta aatcatgtgc aattatttgg tatggcacta catgtagaaa
    10741 atatagataa taaaattaag gaggtagtat gaaggaaagg gctaaaaaaa tattagattt
    10801 tattattaaa aataacaatg taacatcaca agaactacaa gaaaagttta atgtttcaaa
    10861 gagaactatt tattacgata ttctagctat aaatgaacaa ctaggaaaaa gtggtaatat
    10921 aaaaaatgta aaacacaaat ttatatttga aggtaattta tgtgatgcaa ggaaaataat
    10981 ttcaactgaa gaagataaat ttttagatag tgattacaga aaaactttta tattaaacaa
    11041 gatattactt ggcgaaaaaa tatcaataga aaaactaaca aatgaaatgt tactttcaaa
    11101 aaacacagta gtacaaacaa taactgatgt aaaaaaatac cttcaaacaa tggggttaag
    11161 attagagtat aagggaaagt acaaaattat aggtgatgaa tacgttatta gagagctgtt
    11221 tctgataatt gttcaagaaa atatattgga aattaattct ataagtgagg aagtaagtag
    11281 tttcgataca aaaggacata taaaacttac tgattattct ttattaaatc taactaagtt
    11341 tgtggaattt ctaaataaaa ggataagaga tgggaaaaca ttatactcat ataaatatct
    11401 aaatgaagct aaaaaaatta gttactttag taattgtaaa gaacttttat gtgaagaagc
    11461 taatgaaaat gaacaagcct atatatgcac ttacataagt tcacttccca gcttaaatag
    11521 tgaagttaag gaagatgttg ttgaagaata tgtagataaa cttatagata agtttgaagt
    11581 aaatacagca ataaaattag aaagtaagca tgagtttaag aaaaatattt taaggcacct
    11641 ccattcttct tacaacagaa tacgatttaa gtttcctatt agaaatccaa tgttagatga
    11701 gactaagtat aaacatgagt cactttataa aattataaaa tcaattatag aaaatgaaga
    11761 agagtttcct gtatttgagg ggataagaga agaagaaata ggatttatag cagcgtattt
    11821 tggaggatat ttaagagggt ctagggataa tggtctaaga agaaataaag tcttactagt
    11881 ttgtcctaat ggtcttatgg tatcaaagag tttggaaata caactatata aatacatacc
    11941 aaccatagaa atagtaggta tagtatccat taagcaacta aaagaagtaa atgtatacta
    12001 cgattacata attacaacta tagatataca aaatgtaaac aacgtaattg ttgtaaatcc
    12061 tcttttaaca tcatcagatg ttcaattact tatgaacaaa ctaataagtg taaaagaaaa
    12121 cgagaagtat ttcaatcttg aattaataat acaagctata agaaaaaatg gagttatcaa
    12181 taatgaagaa gcactaaaag ctgatttatt aaacataata cacaagatag atgaaggaga
    12241 gatgtatcaa ccaatgttaa aggaattaat taatgcagaa agggtcaata taataaaaag
    12301 tgttagagat tggaaagaag caataaaaat agcctcaaag ccattgttag aagataactc
    12361 tatagaagag ttatatatag aaaatatgat aaagtcagtt gagaaatatg gaccatatat
    12421 tgttttagct gatagatttg ctctaccaca tgcaagttct aaagaaggag taaacaagct
    12481 tgctatgtct ttactaatag tagaagatga agttgattta ttaggaaaac cagtaaatat
    12541 atttatggta ttagctgctg tagataacac tactcacatt agagcattag catcactatc
    12601 agagatgatg tacgaggaag aaaatgtaaa actaataatt aatggtgata aaagttcaat
    12661 actagaactt ataaataagc aaaattagga ggaatgaaaa atgaagatat tagcagtatg
    12721 tggttttggt gtaggttcat caatggtatt aaaaatgaca atacaaaagg ctctaaagca
    12781 gttaggtata gaatgtgaag tagaaaatac agatataaat tcagctagag gtgctgattg
    12841 tgactgtata ttcacaagtc aagaattgtt aaatgaatta aaaggaactt gtaatgtaga
    12901 tgtatatcca gttaaaaagt acatggattt agcagaagtc acagaagttg ttcaaacttt
    12961 tttaagtgat aaaaatgata agaaatagta taagttttaa aaagtaaatt taagttaatc
    13021 agaataaaat aaaaacttac taaattcaaa aatagtaaga aagagaggag ggatttagtg
    13081 gtagaaaact taaacagttg acctatcact aaaaaatata atgcttgatt ttatttcaaa
    13141 taatatttta cgtaatccat ctatgttaat tggattaata gcagcatttg gtcttgtact
    13201 acagaagaag aaagcatctg atgttataaa ggggtcacta cttgcagctt tcggaattat
    13261 aattatggag actggaacag gtatgttagt tagttcaata gcaccaatta atggagcttt
    13321 ccaaagtata agtggtggtg cagtaacaaa aggtttaagt gatgttactt ttactgcaag
    13381 ttatggtgga attataggtt tagcaatgtt tgcgggttta gctattcacc taatgatagc
    13441 aagatttact cctattaaga caatcttttt aacaggtcac atgctttatt ggttcccatt
    13501 catatttgta gcttcaggag ttgaagctgg attaaatggt ggagtattaa tagcttttgc
    13561 agcagtatta tcagcactct attggtcaat aatgccttgg ttattaagaa aatatgtgtt
    13621 tgatgttata ggagatgatt cttttacatt aggtcatcca tctggttgtt tagcacttat
    13681 atcaggattt gttgctaaat tagtaggaaa taaagaaaaa tctacagaag atataaacat
    13741 acctcaaagt ttatcatttt ttagagaaat atctataagt ggtggaatgg cagtgttttt
    13801 aatgtttata gttgttggga tagtaatacc aggtgcgttt aaagatagta atgagtttat
    13861 tggaacgtat gcaataaagc aaggtctttc ttttggagca ggtcttataa taatgcttca
    13921 tggggtaaga atgctaataa atcaaataat gccagcattt caaggtatat ctgaaaaagt
    13981 tgtgccaaat gcactacctg cttttgactg tccaatatta tttaattata aaccaaatgc
    14041 tgtattaatg ggattcttag ttgctatgct aacatctaca gctatgattt taatatgtaa
    14101 ctactttaat gtatttaatt tgctacttat accacttgtt ataacttcat tctttgagtg
    14161 tggagcagca gcagtaatag gagaatcaca aggtggtata cgtggagcta taataggtac
    14221 agctatagct gcaatattta tggtagtttt agtaggaata tctgctgtaa tttactcagg
    14281 aactattcaa aattggatac taatatttgg aggtaatgac ctttcattat ttggtataat
    14341 cagtaggttt ataggttcac tatttggagg aatattatag atatgaaatt taaatacata
    14401 gatgagataa gaagattaat tgatataata gaaaacgaag aaaaagtcaa tatggaaaaa
    14461 gcagtacaaa tctttgttga agcaatagaa agtaaaaatg ctatattttc atttggagct
    14521 tcacatgcag gaatattaac agaagaatta ttctatagag caggtggctg tgttcttata
    14581 aatccagtat ttgcaaaatc tctaatgtta gatacagcgc ctataactca cacaagtaag
    14641 atggaaagat tagaaggata tggtacagct ctagctgata aaactccaat taaaaaagga
    14701 gatgtagtta tagttcattc agtatctggt agaaatccag taagtataga gtttgctatg
    14761 aaagctaagg aaaataaggc aatagtaata tgtataacta atttaagtta ctcaaaaagt
    14821 gtatcttcaa gacattcttc aggaaaaaaa ctttatgaag tatgcgattt agtgatagat
    14881 aatcatggtc aaaagggaga tgcctgtgta gagatagatg gattaaacca aaaagttagt
    14941 ccaacttcta ctgtaatagc aactactgtg atgaactcta ttatagctca agctactcag
    15001 gaattagtta ataaagggct aaagaatcct ccgattttct atagtgcaaa tattgatggt
    15061 ggagatgagt taaataagaa gatttttgat gaatataaaa gtgtaattca ttatgaatat
    15121 taaggttaaa aaaatatgtt aatgaacttt gaagtttatc aacaaagaat attaataata
    15181 tataataaat gctatcattt aaaagctgta ggtaaaaatc tacagctttt tattattgta
    15241 gattaaaaag atgcgtaatt aaattttaaa ttggagaaaa taaattggaa ggtatcaatt
    15301 ataaatatta gaaacgttgt catagaaaaa aactatatta aaaaaaagta catagaaaaa
    15361 actgattaga caataaatac atgctttgat aatattttaa atggagtgaa aatgtgtaga
    15421 attataatga aaaaagaaaa taaaaatgaa aaaagaaaat aaaatggagg gaataaaagt
    15481 gggtatgtta aaaaaattat gttcactagc tatggtagca attatgacta caacacttat
    15541 aacagggtgt tcaagtggaa atggaaaaga ttcaaaaaag gatggcgaaa aagaaaaact
    15601 agttctttgg atgccaccag tagaaaataa tatgaaggaa gtttgggacc ctattttaga
    15661 taaatttgag gaaaaaaata actgtgaagt agatttacag ataattcctt gggataatta
    15721 ttctgaaaaa tttgcaacag ctataagtgc tggcgaaggt ccagatgtag gatatatgta
    15781 tgcagaaatg tatccacagt ttatagaaag tggagcagta gaagatttga ctaattatgc
    15841 aactaaagag gataaagagc aatctattta tataaaaaca tctgaaatga tgggtggaat
    15901 gtatggtatg ccattccaag cagcaaatcc aggggtatta tactataaca aggatatttt
    15961 agagaaatta ggagaaaaac ctcctaaaac atgggaagat tttaaaagaa tttgtaaaaa
    16021 agcaacacaa gatactgatg gtgatggaaa gatagaccaa tggggattag ctcagggatg
    16081 gggagctaaa acttttggta acttaaactg gaactggtat ccttatttat ggcaagctgg
    16141 tggagatatc tttaatgatg atttaaaatc ggttaaattt aatgataaga gtggtttaga
    16201 agcagctaat ttcttaaaag aattacaagc atatgtgcct gaagattctt tatctaaaga
    16261 tagtaatgaa atgatagaaa gtgtatttgg accagggaaa gcagcattta caataatgtt
    16321 atcttctgct gcaacaagtg tatttgatga atcattcccg gatttaaatt ggggatttgt
    16381 aactggacta gaagataaga aagcagcaac ttttggagca gtagaccatt tatctttaat
    16441 gtcatctgct aaaaataaag aacttgcata caagttaata caacatatgt taagtgttga
    16501 atcaatgaca gaatttcata aagcaatacc aagagcacca ataacaaaag gtgaaccata
    16561 tcaaggtgat gaaagattca aagaaatggt ggaaaatgac aaaaatgtat atagaccatt
    16621 agtagtagga cctcatggag ttgaaatata tgaatattta tggaaagaac ttcaaactat
    16681 gatttcagga gataaaactc caaaacaagc gttagatgat gctgcaaaat attcaaatga
    16741 tttattagca caataaaagt ttaaagctgt aatatgttgt aaaggacttt acagattaaa
    16801 attagcattg tatttataaa tacaatgcta atatttatat tggagaggat aaaatggaaa
    16861 acttagttag agagtttgaa gaaaattcaa aaagaaaaaa aacaaaatta aatattaaac
    16921 catatatgta tatattgcca ttgggaataa ttttagtggc gttttatgtg ataccaataa
    16981 taatgtcaat atactttagt tttacaaagt ataatattat tagtccagct acttttatag
    17041 gtttagaaaa ttataaaaaa ttatttacag atgaaacatt aaaagtatct attataaata
    17101 ctattaaatt tactgttgtt gtagtacctt gccaaactat attatcattg attttggctg
    17161 tttggattac aggaaaagga aatagtaaaa tagcttcatt tgctaaaggt gctatattta
    17221 taccagtatt atcatctatg gttttgatag gtatggtatg gagggcttta ttgaatggag
    17281 aaggttctat tatatatcag gtattaggtg cctttggaat agaatcatct aaacttttag
    17341 gagatagtaa gacagcatta cctacactta tgtttatatc tatgtggaaa aatatagggt
    17401 atttcatggt aatttatatt tcagctataa tgaatttacc aaaacactgc tatgaagtag
    17461 cgaaggttga tggggctaca aaacttcaag aatttataaa aataactgta cctttattga
    17521 aaccaactac aataatggtt gtatttttag ggtcgatatg gtcattacaa gtatttgact
    17581 taatttatac tgtgacaggt ggaggtccag gtatatctac tatgagtata gttatgcatg
    17641 catttaactt aaactttaaa aactttaatt ctggatatgc aatgactgta gcaaacgtat
    17701 tatttttgtt aatagcagta gtttcgattt tacaaaataa gcttgtaaga cgagacaatt
    17761 cagattttta gaaaggtagg tataatatga aaacagcaag aagattatta agtgatggtt
    17821 tacttttatt tatagcgtgt gcaagtttgg taccttttat atacatgttg ataatatcat
    17881 tgaagataac atataactca tatagtttgg atatatcttt ttcaacagta acccttcaaa
    17941 actatataga tatttttact aaaaaaggat ttgcacaata tttttttaat acagcaatag
    18001 tatctttttc aggtgtatta ttaaatttag tatttagtac attagcagga tattcttttg
    18061 cgaagatgga ttttaaaggt agtgataagt tatttttatt tatgataatg actttaatta
    18121 taccttccca agttacaatg ataccactat atataataat gaaacactta ggatggataa
    18181 atagttatct tgcacttatt atgccaatac caactgcatt tggcgtattt ataatgagac
    18241 aagctatttt aggagtacca aaagagttat tagaatcagc aaagatagat ggttgctcgg
    18301 attttagaat tttaatacaa atagtgttgc ctttaattaa accagcatta attacactag
    18361 caatatttac ttttatggga gcatggaacg agtttatgtg gcctcttata gctacaacta
    18421 aagatgcaat gagaacatta acagttggat tatctactct taagacattc cagataacta
    18481 attatggaca gatgatggca ggggctacaa taacattttt acctccattt attttctatt
    18541 tgattttaca gagtaaattt gttgaaggtg tgtctttgtc tggtataaaa ggttaaatag
    18601 tctaatttaa taatatataa agaaacttat ataaatatgg aggaatttaa actatggaaa
    18661 tttcatatgt actgaaagat tgcacattca aacacaataa atactcagtt gtagaagctg
    18721 aaacttggaa agataaggat ttggatattg tagtagctaa aggtgaaaaa tttgcatttc
    18781 aaattatgtt aaaagcaaca gaagaattta attgtacaat tgataaaagt agtgctatat
    18841 catggaaagg cctgggaaat agagtaaggt tagcattaaa tatgcctaat agtttagaaa
    18901 ataattttag tataaatata ttaggatatg ttcaagatga tacaaaggca tttgtgaatg
    18961 atgctatttt aagggataaa gatgtgttag tagagcaata tttgcctcaa acattttgga
    19021 ttgaaggtca agtaccagaa gattttgaag aaaataacct tgatataggt attgatatat
    19081 ttaaaagctt tggatatgaa gacgaagaaa aagtttgtac aattgatgtt cctgtaaaag
    19141 taagaaatgt tgtgctaaag cctttagatg aaagcaagtt ctttttagat ttatggcaac
    19201 atccaagctg tttagctaga atgtataaag ttgagttatg gtctgatata cattttgaaa
    19261 tagtcgataa ctatttaaaa gaattagcat ctttaggaga aaaagttgca acagttatag
    19321 tttcagatta tccatgggca gggcaaagtt gttataaagt ttataaaaat ccatcaaact
    19381 tatatgaata taacatggta agtgtatcaa aaggattgga tggaaagatt aaatgtaact
    19441 ttgagagtat ggataggtat ataagcatag ctgataagta taaaatggct aaggagatag
    19501 atttatttgg tctattaggt aattggtgtg ctggtgagtt tggaaatcct gtagaaggtt
    19561 ataaggaccc tataagagta agatattttg atgaaaagga taaagtattt aaatttataa
    19621 acaatacaaa tgatttaaaa gaatatatag gattagtatt aaatcattta atagagtgtg
    19681 gattatggga cagagtaagg attatagctg atgaaccaaa taatccagaa gtagtcaaag
    19741 aatgtataga atttataaat tctactgtag gtactcatca agttaaatat aaatcagcaa
    19801 ctcacgacca aaacttttta gacagagcta aagatgaaat tgatgatatg tcaataaatt
    19861 taaaacttac tatacaaaat tataaagata tagaaagtct taagaaaaaa atcaatgaca
    19921 agggtgggat attgacttgg tttgtatgct gttttccaga gaaaccaaat agttttttaa
    19981 gttctccttt tgtagaaaat agaataatag gatggtatac ttattacttt ggtcttgatg
    20041 gatttttaag atgggactat aatttatgga cagaagaccc ttggaaagat tcaagttaca
    20101 aattccctat ttggaaagct ggagatatgt tctttgttta tcctggaaaa gatttaaagc
    20161 cagttagaag tgtcagaatg gagaatttaa gatttggaat acaggacttt gagttattta
    20221 caatgttaga aaaagaaaag ggaagagagt atatagaagt tgagcttatg caagaattat
    20281 taaataaaaa agaaaatgct gaaattaaag gttttggtga catagaacta ggatattcac
    20341 ttgataattg gggatatgat aaagttaaaa aatatgtatt agagttgtta gacagagtat
    20401 aaaaaatata agagtgtctc aaaatgaact ttttagttca tgaggcactc tttttgtatg
    20461 aaatcaatat agtttcattg tttcaacaat gaaaaatagg attatttcta ttctaagaca
    20521 gccctttagt tttttagttg tattcaatgt tttaaaacta actataaacg ctataaagtt
    20581 tataaatgac aataattata tataacatga tgtactttaa tttgctataa tagggattga
    20641 ataatattat atggaagata acattgtttg gggggattaa tataaaaaga ataggtatta
    20701 ttataatgtt ttttttaagt atatctttta ttggatttta tcttgtaata agagataaca
    20761 gtgatacagt taagatgcaa cgagggataa atataggtaa tgcactagaa tcacctaagg
    20821 attttccgtg ggatgtaaaa atgtcaaata agttttttga tgatataaaa gatgcaggtt
    20881 ttgacacagt acgtatacca gtaaggtttt ctgattatac gtcagattct gataatttta
    20941 aaattgatga agaatttttt aaaaagattg ataaatatgt agattatgca ttggataaag
    21001 acttaatagt tgtgttggat ttacaccatt ttgaagaaat tatgaaagaa ccaagagttc
    21061 ataaagaaaa gtttttaaaa atatggcaac agatagctaa taggtatcaa aaatatgata
    21121 aaaaattggt atttgaatta ttaaatgaac ctaaagaaaa tttatattca cagttactaa
    21181 acgaatacat tgaagaagca ataaagataa ttagaaaaac taaccctaaa agaactatta
    21241 tagtaggacc atataatttc tatcaaattg attatttgaa tgaattaaat attcctaaag
    21301 attctaatat agtagtttcc tttcactatt acgagccaaa tgattttgca tttcaaggaa
    21361 atatatatca taagggattt gagcacttaa gcaatattac ttgggaagga acaaacgaac
    21421 aaatggatta tttaaaaaaa aggtttgata ctgtggaaaa ttgggcaaat aaaaataagg
    21481 taaaaatctt cttgggtgaa tttggaataa caaaagaggc accagaagct tcaagaagag
    21541 catggataaa agctgtaaga gaagaagctg aaaaacgaaa cttttcatgg gcttattggg
    21601 agcttgcatc tggatttggt atatacaatc aaattgaagg gacttgggat agagatatat
    21661 taagtgcatt aatagaaaag aggtgatttt atgaagaaaa attcaaaaaa gatacttata
    21721 actttaacta ttattactaa tgtcatatat atattgtgga gaatttttta cacagtacct
    21781 aaagaagaag ggaagtttgc cttaatatgt gccataatac ttctttttgt agagataatg
    21841 ggcatgatgg aaatgtttgt gcattattat ggtatgtcaa atattgagta ccctgaaaaa
    21901 ccaatcataa gtgaagaact ttatccacat gtagatgtat tcattgcaac atataatgaa
    21961 tctgttgatt tggtgagaaa aactgtaaat gggtgtattc atatgcagta tccagataag
    22021 aaaaaggtac atatatacat atgtgatgat ggaaatcgtg aagaaatgtg tattctagca
    22081 gaaaaaatgg gtgtaaatta cataactaga actgaaagag aaggagcaaa agctggaaat
    22141 ttaaataatg caatgcaaca tacaaattcc cctttgattg ctacatttga tgcagatatg
    22201 ataccaatgc atgacttttt aattgctaca gtaccatact ttttgaaaaa tgagcaagct
    22261 aaaaaagatg gagaaaaaga ggagtatgaa aaggttggtt ttgtgcaaac tccacagagt
    22321 ttttataatc cagacttgtt tcaatttaat ctgcactctg aaggaaggat tccaaatgag
    22381 caagattatt tttacagaga cattcaattg gctagaaata ggacaaattc tgtaatttat
    22441 ggtggtagta atacagttat ttcaagggaa gcacttgaag aagttgatgg attttatact
    22501 tattctatta cagaagattt tgcaacaggt atcttaattc aaagtaaggg gtatagatgt
    22561 tatgctattc cagaagtaca tgcatcaggt ttatctccta cagatttaaa aagtcttatt
    22621 aagcaacgtg aaagatgggc acgtggatgt atacaaactg gtagacgtct aaatattttg
    22681 tttaggagag gtttaggttt ttggcagaaa ataagttata tttcttcaat tacttattgg
    22741 tatgcaagta taaaacgctt tgtatatata atggcaccta ttttattttc tgtgtttaat
    22801 gtgattgtag taaaatgtac gttacttcaa gtattggtat tttggttgcc aatgtatatt
    22861 ttaagtagtt tatctcttaa aatattttct caaaatataa gaaatactag atggaccaat
    22921 atatatgaaa ctataatgtt tcagtcttta atgccagcag taatacttga aacgtttgca
    22981 atatctaaaa ataaattttc agtaacaaat aagagtaaat tagaagaaaa tagaatgtat
    23041 aagtttttac agggaatacc ttattttatt tacatggtct tatctatcat aggaatttta
    23101 aaaatgtttg tagcaatatt taaaatgagt tcaatgacat attcagttgt attattttgg
    23161 ttgattggaa acctttttaa ccttgttatg gctacattat ttatatcagg gagacagcag
    23221 ttaagaaagt ctgagcgtta tattgctgaa attgatttta agttaaagca aaattcttat
    23281 gttctttctt caaaaacaat tgatatatca gaaaatgggt ttgcattttt gctagaaaat
    23341 ccagaatata tttccccaga agaagagttt gaggtagagt ttagagaaaa atctggaaat
    23401 gaaatgtata tagctaatat gaaagcaaag attgttaatg ttgtggaagt aaattcaaag
    23461 tggaaatatg cagcttatat tacacatatt gaagatagtg aaatagataa ttggatgtgt
    23521 attgttcacg atagaatacc tactttacca atgacaatat ctaatcaact aggtttcttt
    23581 gatgatttgc aaattaatgt aaaaaaacgt attgaaaaaa ctagaacatt atcacgaaga
    23641 agtccaagaa tcaatatgaa ttttcagatg gatataaaaa atacaggtaa attaagaata
    23701 gtgaatttta attatcaata tgtattatta aattttgaga ataaaagtat ttatcctaaa
    23761 gaaatagcat tggaaattaa tgaaaatatt gtgttagaat gtgatttgtg tgagggtaaa
    23821 attgatgaaa gaggtatctt atataaagta aataacattg acagcataat gcaaaatttc
    23881 tttttaagag atgaaatgat ggattggata ttacaaaata agaaaattct tgattcaaag
    23941 cctagtgaaa agaaggaaaa aagtatagat gagtttgaac ccatggaata tatttaggag
    24001 agatgaaatg aagactttaa aagtgttttt ttgttctaca aaatatctta ttttggtgtt
    24061 tatttttttt ataaatacta cttttataag ttcagcaaca agtgataaaa ttttagattt
    24121 atcctttaaa aagatagaga ctgacttaag ttctaagatt acatatgagg atacaggtgt
    24181 aaaaatagaa acggatagtt ctaaaagcga taaggaacgt tatttatata tttaccaaaa
    24241 tataaaagaa aattggagca tgtataataa tttttatatt gaaattcaaa ataaaaataa
    24301 aagttctcag agaataaatt taagcataca aagtaaaaat atgcttgaat ttagattaaa
    24361 agaaggtagt gaagtatttt tagaaggtaa aaatataata tattcagata aaataaaaga
    24421 aggatgcata gaagtaccag gtgaatttga aggcaaaatt tatgtgaact ttaatagcct
    24481 tataaatgag gaaagtaatg ttgtgctgga ctcaaatatg ctttcaaaca ttgttagttg
    24541 gggaattacg tttattccat ctgatgaaga acataatatt gttattataa aaaagatatc
    24601 tttattgtct gaagagaaat taagattttt aaataatatt aaaataattg gcgatgaaga
    24661 agtacaaata ccagtattgg gtcaaagtat aagtcagtat gaagtattag gtcttaaaag
    24721 tgattctaaa attaagtact ctcttatggg aaaacaagat aatgtaagca tatctcaaaa
    24781 aggtaaatta acattaagta ataaagccaa acctggacaa attattttac aagttaatgt
    24841 tgatgataag tttaaaattg gaaagaaaat aacattaact gagtcttgga gtgttaataa
    24901 aaaagataag gatggtgttc catatacatt agtatatcct gagcaatcac caacagtaca
    24961 agatatgaaa aaaataaact ttatgaataa catcataacg tttgttcgaa ttttatttgt
    25021 atctctagta ataatatgct ttggaatata tttatattgg aaaaaatgtt caaaaactaa
    25081 gtaaagagag gaggttagat tgtgttattt tcaagtttaa tttttttatt ttatttttta
    25141 cctataactt tggttttata ttatgtattt aggtttaacc gaacgataca gaacatgata
    25201 ttgcttgctg ttagtttatt tttttatgct tggggagagc caaaatttgt tgttatcatg
    25261 atagtttcca taataatgaa ctatatattt ggtttactgg tagatagata tagggaaagt
    25321 aaaataaaag taaaagtttt tttagttttg atgtgtgcat acaatatagg tgttttattt
    25381 atatttaaat atctagcttt tgcgctaaga aatattagtg aacttattag tactgaattg
    25441 actataccta gtattgtttt acccatagga atttcatttt ttacatttca gggaatgtct
    25501 tatgttatag atgtatatcg tagacatgga gaagtccaaa agaacccttt ttatgtggga
    25561 ttgtatatag catttttccc acaattaata gcaggtccta tagttagata tgagagtgtg
    25621 gcagaacaaa ttctcaatag aaaagaaact tggaataaat tttctattgg gacttgtagg
    25681 tttataacag gtctaggtaa aaaagtatta atttcaaata acatggctat agtagcagac
    25741 tatatttaca ctatgaatag tcaaggagaa atagctgcat cacttgcatg gcttggttca
    25801 atagcctata ctctgcaaat attctttgat ttttctgctt attcagatat ggctataggc
    25861 ttaggtctta tgtttggttt taaatttgaa gagaacttta attatccata tatatcaaaa
    25921 tcaattagtg agttttggag aagatggcac atttctttgg gaatgtggtt taagagctat
    25981 atatactttc cgcttggagg ttctagagta gcaaataaag atattatgat tagaaatatg
    26041 tttatagtgt ggttatttac tggaatatgg catggagcag aatggacatt tgtaatttgg
    26101 gggattttaa actttgtttt cctaattata gagcgattta taatgtttga aaagattgaa
    26161 aatcacaatt ttataaaaca tatttatgct ttattagtag ttaattttgg gtgggtatta
    26221 tttaggtcac ctaatttaaa agaagcatat aattatttta aagctatgtt tggaggcaat
    26281 ggagctatat ggagtgatta tacatatatg tttttaaaag aatactgggt tttctttata
    26341 tttgcattta tttttagtgt acctattgca aagaaaatca ataagtttgt tgttgaacaa
    26401 gctaaatata gtatgatatt taacgtattt tatccaatct cagctatttt attgtttttt
    26461 atttctgtga catatttagt aactggaagc tataatccat ttatttattt taatttttag
    26521 ttgggaggaa aagtatgaat agatttttta aagtaagatt aataactgca attatgttct
    26581 taattatttt attcgctttc tctatcttta atgtttgtta ttctggaaaa gatacgatac
    26641 aggaaataaa aaattcaata gctgaaaaaa gtagtttaaa agatacaata tcatctattg
    26701 ataatacagt gaatgacaaa gtaatataca aaaatacgtt cattgaaact tatggctatt
    26761 tacaaaaatt aatgggtaaa aatgagtttt caaaatttag tgttgtaaaa gatacttctg
    26821 gaaagatgca ttatacatat tttgctaaag ctccaaatcc agttggtgtg atagcagata
    26881 gggtaagaaa attttctgat gaaatggaaa agcaaaacac taaattggtt tacctaatga
    26941 ctccagataa gtatataaaa ggagtgacaa aatttccaaa aggaatacct tattcttata
    27001 gtaatgagac agcagataat tttttaaatc aactaaaaaa gtataatgtg gactatattg
    27061 attttagaga aaatatttta aagagtggta ttccaaaaga tgatttattt tttaaaacag
    27121 accatcactg gaaagtagaa acttcttttt gggcatttgg agaattagtt gagcaactta
    27181 ataaaaaata caatatgaat ttagatgaaa atcattatta tagagataag gacaattata
    27241 attcaattgt atatcctaag tcatttcttg gttctatggg aagaaagact ggtatcttat
    27301 atggtggtat agatgatttt acattaattt atcctaaatt taagactaat tatacttatt
    27361 atacagattc taaatctcaa aaatttgaat taaaaggaag gtttgaagag agtttaatat
    27421 tatcatatcc ttttaatgca gaccttgatt taatggatgg tcaatcagat aagtatttta
    27481 catatttatt ggggaacaga ccattagtta aaataaaaaa tatagaaaat ccagatggat
    27541 taaaagtgct gtttgttaaa gattctctaa ttgtaccaac agcagcattt ttttcctctg
    27601 tttgttcatc tatagatatg atagacccta gatattatga tggagatata ctagaatatg
    27661 caaaatccca tgactatgac tttgttttta tatctgtata tccccaaaat cttacaaaag
    27721 aattttttcc gttttgtgag taaatttcta aaaaagtttt ctgataaaat aaatggctaa
    27781 ctggtaaaaa gttagccatt tattttgagt gttattctaa gaaatatttt ttttactaaa
    27841 tacttcttta tctatttttc caagtgattt tcctacagta attgatgata ccatagcacc
    27901 attaacattt atcatagtac gtcccatatc tagtataggg tctattgcaa gtataccacc
    27961 aactaatgga aaataagaac caagaccaac tccagagata acaactgaaa cggccattgt
    28021 agcagttcct ggaagaccag ctattccaaa cgaactaatt gttataacaa ttaacaacat
    28081 aacataaaag ctcatatcca tgtttactcc agccatattg gcaagtgtta cagccattaa
    28141 agcaggatat atacctgcac aaccattcat acccatattt gcacctaaac ttgctgtaaa
    28201 gcttgataca ccctcatcaa ctccgtgatt ttgtactaga ctttctatcg ttacaggcaa
    28261 tgttcctaaa ctagaacgtg aagtaaatgc cagaagtaaa gcttcagttg aattttttat
    28321 ataagtaatg ggatttagac catttaaagt tataaaaatt aaatgtataa taaataatat
    28381 tgcaactgat atataaagag ctaatataaa ctgtaaaaca gatactattg aactaatacc
    28441 ttgtgaagtt attgtatttg caagcagagc aaccacagca taaggcataa atttaataac
    28501 agtcatagac acacttacaa ttattttgta agatgcttct ataaaaccta taaatggttt
    28561 tattgtttca aaatgttttt tacttaatct ctttattgag gtagctataa atgtagcaaa
    28621 tattattgta gctacaatat ttccatttgc catagccatt acaggattag atggtagtaa
    28681 tcctcttaaa gtatccacaa gagagtttat ttctttaagt tcttgatttt caactaaagt
    28741 aggcatatta gctcctacac ctaacttcat tatatttgca acgataattc caacaattgc
    28801 agctatagca gtagtagcaa gaagcatacc taaagattta aatgtcagag ttccaagatt
    28861 gttatctttc atatttataa ttacttttat aatggataag aaaactaaag gtactactaa
    28921 catttttaat aagtccataa atccagaacc aaataggcta taccacttac ttacttcttg
    28981 tagccaaatg acttgagttg tatcattagg aaaaccagca aaagcttgta ttatgattcc
    29041 caatatgagt cctagtactg tagcatatat tgttctcaat gaaaatttta tttttttctt
    29101 ttcaaattgt ctaacaataa agaacgtacc tagaagtaat attatgaaaa aaattgtttt
    29161 aaaatctgta atcataagaa attgtgataa aaatgaactt tccaatattt ttctcctttt
    29221 atttaaaaat aaatttttct aagtttaaat gatatcaata tattaaaaaa atgtcaatat
    29281 tatatcacaa tttacttaaa tagagtaaaa caagttgatt ttgaaaaatg tgattaagta
    29341 cagaaaaata gggtataata aatgtttaaa gaatttgaat tgtggtaaga aaataaaaaa
    29401 tagcttttat aagatttgat ttcaaattaa aatgtgttaa ataaataact aaaaattatt
    29461 ataaaaaatg ttttcataga atatatatca aatgaaaagc ttaaaataag tgaagaaatt
    29521 aactagacat caaaggagag ataaaatgag agtaataata attggtggtg ttgcagctgg
    29581 tatgagtgct gcagcaaaat taaaaagaat aaaacctgag tatgaagttg ttgtttatga
    29641 aaaaactgaa atagtatctt ttggtgcttg tggattacca tactttgttg gtggattttt
    29701 taatgatgct gatgaattat tagcaagaac accagagaaa cttagagaag ctggaattga
    29761 tttaaatata tttagagaag tcgttgaggt tgatagtgaa tctaagaaaa taaaagtaaa
    29821 aaacataaaa acagatgaaa tatatgaaga ttactatgat aaattgatga tagcaactgg
    29881 agcaagaagt attatgcctc ctataaaaaa cataaaatta aagaatgttt ctacattaaa
    29941 aagtttatat gatggagaat atttaaaaaa acttttaagt aatgaagata ataaaagagt
    30001 gacaataata ggagctggtt ttatagggtt agaagcagtt gaggcatgta agaaattagg
    30061 taaagatgta gatgtaattc agttagaaga tagaatattg ccacaagtat ttgataagga
    30121 aatgacggat gtattagaag aagaaataaa tagacataat gttaatttgc acttagatga
    30181 aatggttgta gaactttgtg gagaagataa agtagaaaaa gttataacaa acaaagggga
    30241 aatagataca gatgtagtaa taattgctac tggcgtgaga cctaataccg agtttttgtc
    30301 aaatactaat ataaaaatgc taaaaaatgg agctatagta gttgatgagt atggaagaac
    30361 ttctgtagaa gatatatact ctgctggaga ttgtgcaact ataaagaata tagtaagtaa
    30421 tgaaaatgta tatgttcctt tagctactgg agctaataaa ttgggtagga tagtaggaga
    30481 aaacctagca ggaagagaaa tatcttatca aggttcacta tcttcaagct gtataaaaat
    30541 tatggatatg gaggcagcat caactggaat tacagaaagg caagctaaag atttaggcat
    30601 aaatgtcaaa tctaaattta tttctgatta taatcaaact cattattatc caggaagaaa
    30661 taaaatatat gtaaagctta tttatgatgc agatacaaaa gttattttag gcggacaagt
    30721 ggctgggttt aaggatgctg tccaaagagc aaatgtattg gcagctgcta tctttggaaa
    30781 aatgaccaca aatcaacttg gaatgttaga tttatgttat gctccaccat ttgcaagaac
    30841 atgggatgtt ttaaatgtag ctggaaatgt atctaaataa tatttagatt atagctaata
    30901 tagttgacaa gcaattaagg gaatgaattt acctaaacta tttttatcat ggtaaaattc
    30961 attcctaaat ttttattcca aatttttatt ccaaattttt agaaataagg attccattaa
    31021 attattcatc aaaatttaaa cgcaagctta atctatcttc tccaagaata gtattttcaa
    31081 aaacattagt agcattttct aaatacatac taggtatgtc taaagaagga ctaaaatgag
    31141 tcagtaaaag ttgtttgaca tttccaagtt ttgcaagatt agcagcttct ctaaaagtca
    31201 tatgtttgtt tctaacagct tttgatatat ctaaatcatc accatacatg gcttcacata
    31261 taaataaatc actatctttt atgaaatctg gtattgattc tataggtctt gtatcggttg
    31321 taagacttat cttaactccc tttctcaatt ctcctaagac catatctgga gtgtattgtt
    31381 ttgaatctaa aactatattt tgaccttctt ggagtttgtt ccataatatt ttaggaactt
    31441 cgtttttagt tgccttatct atatcaaatt tagggtttct tctaaaatta aaactgtatc
    31501 ctaaacactc tatagagtgt tctaaactga ttgttgaaat ttctaaattt cttaaaactt
    31561 tattatctaa agaaaaattg ccttgagggt tttctataat ttttaaagtg tatggtacat
    31621 attctactaa atttctcata gaacgtatac aatcgactat tccaactgga ccaactatag
    31681 ttaaatcact tgtacgacca ctattcccaa ttgttgataa aagtcctaaa agtccaaata
    31741 tatggtcacc atgtaaatgg gttatacaaa tcaagtctat atctttaaag ccacattttt
    31801 ttagcttcat agatacctga gtaccttcac cacagtcaat tagtattttt ctacctttat
    31861 agtttataaa tacagaggat agaaatctgt ttggcatagg tacatttcca ccacatccaa
    31921 gtaaagttaa atctatcatt ttttcccctt tctaaaatta acacaaatct gttgtttgtt
    31981 tttattatga tatcctatta taattatttt aacattaaat ataacaataa atgtgtattt
    32041 gagtcaagaa atattgtcaa ataaagtttt tatatagaaa tgtataaaaa tttggaaaac
    32101 aaaaagtgaa aattatattt aggagcattt aaaggaggaa aatttaatga aagtattaaa
    32161 gaaaattact attttgtcat tgattacatt aaccttagtt ggttgtagta acggagaaga
    32221 taaaaaagtg gttgaaggtt ctaagaaaat tcaaaattct aaaaaagatg aacagcctat
    32281 aatagtacat aaagatgcaa atatattgaa agataggtat gagccaccaa aaggatttaa
    32341 aagagttgat gtagaaaaag gtagttttga atcatttctt aggaatacta agctaaaaaa
    32401 ttatggagag aaagtaaagt attatgatgg aagaactaag gaatcaaaag gtatttatga
    32461 tagtgtattt gatgttgata ttggcaatag agatttacat caatgtgcag atgcgattat
    32521 gcttatgaga gcagaatact tgtatcagaa taaactatat gatgaaataa gttttgattt
    32581 tgttgatggc tttaaggcga agtattcaaa atgggcacaa ggatatagaa tttcagtaaa
    32641 agatagtggt tcaagctggt ataaagcaac tgaatattca acatcttatg agagttttag
    32701 aaagtttatg gatatagtgt ttgcatattc aggaacgtta tctcttgaaa aagaattgga
    32761 gcctgtaaaa gttgaggata tgaaaatagg agatgtgttt atagttggtg gaagtccagg
    32821 acatgcaagt ataattatgg atatggcaga aaatgaaaag actggtgaaa aggtatttat
    32881 gatagcccaa agctatatgc ctgcacaaca aacgcaactt cttattaatc gtaaaaatcc
    32941 aaatttgagt ccttggtata gtttagattt tgaaggtgat ttgacaatac ctcaatggac
    33001 ttttaaaaga gaccagttga aaagatttta aaataataat tttatataat aaaagaaaaa
    33061 tatagatgtg aaaaaatgta attagattaa aactaattgg atatggacaa aattaaattg
    33121 atagataaat attaaaaatt acactaatta atcttactat agtttaatat ttatctaaaa
    33181 agttttatgt aaaatataaa ttttatatta aagctaaaac taatttgagg aggctaattt
    33241 atattgaaga ttagaaggta tttaattttg gtaattataa ttctaattac aacaagtcag
    33301 agttttattt cttatgggct agagcgattt gaagaaataa ccatttttca cactaatgat
    33361 atacatggaa gatatgctga aggtgatgac catatacaaa taggaaatct agccacattg
    33421 aaaaaagaaa ctccaaactc aattttagta gatgcaggag attgtttgca tggacttcca
    33481 atagtaaata tggatagagg gaaaagtgca attgaattaa ttaaatgtgc tggatatgac
    33541 tatattactc ctggaaatca tgactttaat tatggaaaag atagactgtt tgagttatct
    33601 aaaagtgcta atttaggaga gaatacttta aaatttttat catcaaatat ttttgaaaat
    33661 ggaaaaaaag tttttgatag taatgatata aaagaaatag atggaataaa aataggattt
    33721 tttggactct caacccagga aacaaagagc aaaactggat ttaaaaatgt ggaaggatta
    33781 gagtttaaaa atcctataga aagtgcaaca gaacaagtaa atgaattaaa aggaaagggt
    33841 gccaatgtaa tagttgcaat atgtcatatt ggtacaaata gtttaagtaa gccaaatagt
    33901 atagatatag caaatgaggt tcaaggaatt gatttgatta ttgatggaca cagccacact
    33961 aagtttgaaa atggtaaaca agtgggtgat acattgattg taagtgcagg acaatattta
    34021 gaaaatattg gcgagataaa actaactttg gatactaatg atgtagaaaa tataaaaata
    34081 gaaaataaat cagttagatt aattggaaaa gaagaagcat taaagtataa gcttgactcc
    34141 gctgtaagta aaaaggttga tgaaataaaa gaacaacaag aaaaaatatt agataaagtt
    34201 ataggggtaa ctacaaatac tctagatggt tcatatgaaa atgtaaggac aaaagaaact
    34261 aatttgggta atttaataag tgacatttta ctagataaaa ctaaggctga tatttcttta
    34321 tttaatggag ggaatattcg agatacgata gaaaaaggca atataacaag aagagatatt
    34381 gtagatgtat ttccttttaa caatactata gttaccaaag aacttacagg agcacagatt
    34441 aaagatgtat tggagcatgg agtaaaactt tacccagaaa aaagttcagc ctttttgcaa
    34501 gtgggaggaa tttcatatta ctttaatcct aaacaaaaag aaggcgaaaa aataacaaat
    34561 attcaaaaag aaggaaagcc attagattta aataaaaagt atgtagttgc aacgaatgat
    34621 tatatagcat caggtggaga tgaatttcct tgtttttcaa aagagccagt actaaaggaa
    34681 tttggaaatt tagagagtgc agtcattgaa tatatagagt acaaaacaga aatatcaaaa
    34741 aatgtagatg gaagaattgg tattaaatct aaagatgagt taataagcaa gaatcatgag
    34801 agtgatttga tattaagtga aaatgaaaaa aatatcaatg ataatgaaga gaatctatct
    34861 aataatataa aaagctctga tagtgataaa aataaaagct tagataaaac aagtgctaga
    34921 gaaagttcta atctaggaat atctaaaaga gaagctaaag aaaaatctcc taaaacagga
    34981 gatttaggtt tttcaaatag tataattata tttatagttt caagtacatt gatatgttta
    35041 ttgaatttta atcaaaaaga gttaaaggat aaaaagtcaa aataaaaatt aatgagcaaa
    35101 aactagtaga tagtaattta attttagata atagtaaaca gcaaaggaaa ttagttatat
    35161 aaatactaat ttcctttgtt ttatgtttta acatgattac ttttaaataa aattgtattt
    35221 tatgaaaaat aattacacta aatggcatag tattatgggg atttttagtt tttattcaag
    35281 taaatgaaat catattaaca acttctgata tatttacaaa ttgataaaaa aatgatttta
    35341 ttagtatgat ataatttagc tagtatatat gatgatggag gatataggga tgaataatat
    35401 attacttttg gaagatgaca aatctttaaa tagaggaatt agttttaaat taaaaaaaga
    35461 aggatacaat gtttttagtg catttggtat agaagaagca aaaaatattt tcgcaaaaga
    35521 agagatatgc ttaattattt ccgatatagg tctacctgat ggtagtggtt ttgacttttg
    35581 tgaggagatt agaaagaaaa gtgatgttta cataataatg ttgacagctc ttgatgaaga
    35641 ggtggatatt gtcacaggat atgatttagg tgcagatgat tatatcacaa agccttttag
    35701 cttgatggta ttaatatcaa aagtgaatgc gcttatgaaa agagtaaata cagtaaaaaa
    35761 ttatactcta ttagtttgtg atgatttatt cttttattat atagaaaata aattaattat
    35821 aagaacagat gataaggaag aagagataat tttaagtaag acagagacaa aactattaaa
    35881 atatttgatg gaaaattcta tgcagacatt gactaaagag caactgttag aatctttatg
    35941 ggatagtagt ggtaattttg tagatgataa tacgattgca gtaaatataa gaagacttag
    36001 acaaaaggtt gaaaaaaatc catctgaacc taaatatata aaaacagtaa gaggggttgg
    36061 atatatgtgg ggagaaagga gtataaaaaa atgctaaaaa atatgaacct caaagatggt
    36121 ttaagtgtga aagatgatta tataatgaga agagttattg tatattctgt tttatctgta
    36181 ctaatagtaa cccttgtatt tatacttttt gaatatagca gattacataa attgtatgaa
    36241 catcaaataa aaaatactca aaatattgct ggaatattga ttgaaaaata tcctaatgat
    36301 gaaatagata tagtaaattc tatttataat tctgattata gtaaggtgtc tactggagaa
    36361 aaggcattta agaaatttgg ctatggattg gaaaataaaa tgtcaaatga taaaaatttt
    36421 agcttatact taaaatcttt ttttaaggaa tcatttttta tttttttaat gatgtttata
    36481 gtagttatag gtataatata ttattttata agatacataa ataaaagatt gagtaaaatt
    36541 tattttatag tagataagat gtcacaagaa aactacttga aggacactac tgagtcagaa
    36601 gaaactggtt atctagtaga atataatgaa tactttaaag aaggcatatt tagtaagata
    36661 aataactgtc tatatgagct taatagaagt ttaaagataa agtttattaa attagaaaaa
    36721 gaaaaagaaa gtgtaaaatc attagtaaca gatatttccc atcaattaaa aactccttta
    36781 gcatccttaa aattatacaa tacattactt atagaagaag aattagaaga agatgaagcc
    36841 aacgagttct tattgaccaa taaggcttct ataaataaat tagaaaattt aataaattct
    36901 ttagtgaaca tatcaaggtt agaaattagc atgataaaca taaaaaaaga agataatgat
    36961 ataaaaaata ctattctaaa tgcagtaaaa agtgttaccc ctaaggctaa gagcaaaagt
    37021 ataacattaa atttaaatga atttaacagt agaattatac cacatgataa aaagtggact
    37081 gaggagagta tttttaatgt attagacaat gctgtaaagt atacacaggt aaatggagaa
    37141 ataaatatat ctgtagaaga aactgctaat ttctttaaaa ttataataga ggataatggc
    37201 atagggattg aaaaaaatga gtttaataat atatttaaac gtttttatag aggttctgct
    37261 gatgaaattg aatctgtaga aggtagtggg gtaggattgt atttaagtag aaagatttta
    37321 gaagaacaag gtggaaatat tatagtttct tcaaaagtag gaatagggag caagttttct
    37381 ttgtttctta caactatgta atattaaatg aaaggaaaat gtaagcctct acatttatta
    37441 tataagtata aacagctcca gaaataaaaa attaataaaa tatggggtaa tataacttag
    37501 aatgagagga taattttatg aaaatactat atactgaaaa tttatcaaag cattatggaa
    37561 aaggagaaag cttagtaaga gctttggaca atgttgattt agaaataaat gaaggtgaat
    37621 ttgttgcaat aataggaaaa tcaggttctg gaaaaagtac acttttgcat atgattggag
    37681 gtcttgatat acctacatct gggaaggtgt atatagataa taaaaatata tttacattaa
    37741 aagaagaaga acttgctgta tttagaagaa gaaaaatagg gtttatattt caaagctata
    37801 atttaattcc atctcttaat gtatgggaaa atgtagtgtt gccaatcggt ttagatggta
    37861 gagaggtaga tgaatctttt ataaaagaac ttttgaaatc attaggactt gaaaataaac
    37921 acgacgtttt accaaataca ttatcaggtg gtcaacaaca aagggtggca atagcaagag
    37981 cattagctac aagaccagct ataatacttg ctgatgaacc aactggaaac ctagattcta
    38041 aaacatctga tgaggttatg agtatattaa aatctatgtc taagaagtac agtcaaactc
    38101 tagttatgat aactcatgat gacagtatag cccaaatggc tgatagagtt atattcatag
    38161 aagatggcag ggtgtcaaag gttggtgata aaaatgatta atactactaa aatagcaaaa
    38221 agtaatttaa agcaaaataa atcaaaaagt attctaataa taattactat aatactttca
    38281 accttaatgc tttcttctat tggaatttat attgttgatg caggagcata tcaaaaagaa
    38341 aatactataa agtattcagg caattatcaa ggtatattag caaatgtaga tgaaaaacag
    38401 gctgatatat tgagtaatca tgcagatgtt gaattaactg gtgaaatgaa tggagtaggt
    38461 gttgaaaaac tggaagatga ttcaaatatt tctctagcat acatgaatga agatgctttg
    38521 aaattaaata gttttgaatt tatcaaagga aaattgccat caaaagagaa tgaaattgtg
    38581 ttagatagtg gagctttaaa ggcacttggt tataaaaata aagatttagg tgaaaagata
    38641 aaaatatctt atgatgacta taaaagtgat aaaaaaattg aaaaagagtt cattataagt
    38701 ggaatcttaa aaactagcga aatatctgaa gcaggtaaat attattatgc tattatatca
    38761 gaatcatata tgagaaatac tagagatatg tcgcaagaag attttaatat atatgtgaaa
    38821 ttaaatgata aaagtaattt atcgatagaa caagcaaaat caaaattaaa taagattgct
    38881 aatgatgtag gattggacac tattaatgta gctgtaaatg aaaactatat aaatgcttta
    38941 aaaccagata tggaaacaat tatgggtgga gtatttgtag ggttagtaat tgtattgtct
    39001 agtattttag taatatacaa tatattttat atttcaatag ttacaaaggt tcaagaattt
    39061 ggaaagctta gagcaattgg tgcaactaaa aaacaagtaa aaaatatagt atttaaggaa
    39121 gggttcatat tagccggaat agcaatacca ataggaataa ttttaggcta tgtacttgca
    39181 aatattataa taaaatcatt tatggatata gatgtaaaat catcacgatt accagttata
    39241 ttatcggttg tggtaataag ctttatatct gttgttttat cacttttaaa accaatgaaa
    39301 gtagcatcaa aagtatccat tgttgacgca gtaagatata caggaaataa aataagtaat
    39361 aagagtaaga gaaaaggata taaaaatata aatctaaaga gattatcaca tgccaattta
    39421 gaaagaaata aaaaacgtac ttacatgaca ttggcatcac ttatacttag tggaactata
    39481 tttataacag tatcaactgc cttagaaagt tttgatgctg agaaaatggc aagagaacac
    39541 tttccttatg atatagaagt tagactatct ggttatgaaa tgaacagtga taaaaatcct
    39601 aaagataatt tgaatatttt acaaatggat aatcctctaa gtaaagattt ttttaatcaa
    39661 ataaagaaca tggaaggaat taaaagaata gaatctgcaa gaagtgtaaa aattggtatg
    39721 gaggactatg atgttgaatt taagtatgac cttttacaaa gtataaatga aaatgatgta
    39781 aaatcattaa acaagaatat aatagatgga aaaataaatc ttgaaagact tcaaactgga
    39841 gatgaaatag taattactca tgttgataca gcaaaagaaa tgggagtaaa agcaggagat
    39901 aaaataagat taactttata tgatggagat aaaaagataa aaaaagaatt taaagttcaa
    39961 gcaatagcta tgggggttcc aagttttggt atagggaagg attttattga tagaactttg
    40021 aaatacgatt caactagtag cttgggaata tatacagaag aaggaaagta tcaagaaatc
    40081 aaagatagta tcaagaagat tgctaaaaat aatggatttt tagagactga ttttatagat
    40141 agtagaattg agtcaaataa ggctactatt tcatttataa agataatggg atacacgcta
    40201 acaggcataa taggtgtgat tggatttatg aatcttgtca acacaatgat aactagcata
    40261 gttactagaa aaaaagaact tggtatgctt caagctattg gtcttactaa taaacaatta
    40321 gtgaaaatgc ttaattcaga ggcaatgtat tacacttctg gaatgatgat aggaagtatt
    40381 ctatttggag gtatattagg gtatattgct gttatatttc taaaaaagac tggattatct
    40441 tatgcaacat attctttgcc aatagtacca atcttgttga tgattgtgtg tattcttata
    40501 gctcaattta taacaactta tttgatagga cgaagtttta ataaagaatc tcttattgat
    40561 agagtaagat acagtgaata aaatacaaat atttatggtt agtttaacta gcaaaaaaca
    40621 tataaaaaaa tatagttaaa gagattcata attaaatagg ttaaagcata gtacagagca
    40681 aacaatttaa gcagatgttt gctctgtatt ttattatgta atcaataata tatctaatat
    40741 actgatatta ttcatgttag aaaatgtacc ggtacaattc taaaaaagta taaaattgta
    40801 ccatataaaa tgaaaatttt gtataataat cgtatagaaa ataaatagga gctgatatat
    40861 atgaataaga agattaatta tgcaccagga ccaactgaaa ctagggaaaa tgtaagatta
    40921 gcaagagcag aaaaaacaac aaatccagat atagatattg attttgttga attttataaa
    40981 aagacatgcg agaagtttgg taatataatt ggaacaaaaa acgatgttta catattgagt
    41041 ggtgaaggaa tattaggtct tgaagcagca tgtgcatcac ttacagaaaa aggagataga
    41101 gtacttgtaa ttgacaatgg tatatatgga gaaggtttta aagattttgt aacaatgtat
    41161 ggtggggagt atgtgctttt ttcaagtgaa tatactaaaa gtatagatat tgatgaattg
    41221 aaagcatttt tagatgagga tagtaatttt aaatatgcaa cagtagtgca ttgtgatact
    41281 ccaactggcg tattaaatga tgtaagcaaa atatgtccat tacttaaaga atatggaata
    41341 ttaactgtag ttgattctgt agcaggtatg gtaggagaaa gattaagtgt agatgaatct
    41401 aaaatagata taatattagg tggttcacaa aaagctattt ctgcaccagc aggacttact
    41461 atagtcggaa taagtcaaga tgcaaaaaat tgtataaaaa atagaaaaac tgatgtaata
    41521 ggtttttatt gtaatttaaa tatatgggaa ggctattatg aaaaaaaata cttcccttat
    41581 acaatgccaa taagtgacat aatggggctt gataaagcac ttgataatat attagaagaa
    41641 ggaatagaaa atgtattaaa tagacatgaa aagatagctt atagtgttag aaaatctatt
    41701 gaggaatatg gtctagaact atttttagaa gaaggatatt ctaatacagt cacagctata
    41761 aaaattcctg aaagtatagg agctttagaa cttactgatt atatgcttaa aaattataat
    41821 acacttgttg caacatcact aaatcaatat atggatacta ttttaagaat aggtcatatg
    41881 ggagagaatg caaatctaaa taaaatagaa catgtactaa atgttttgga taaaagttta
    41941 agtgcattag gattcaaagg aaatggaaat ttgttaaatt tatttaataa gtattatttt
    42001 taatagatag ggggatatag tatggaagtt acagctaagc aaaaatttac aacgaaagac
    42061 atggtggaaa cctcactatt aatagcatta gtatttattg caactaaatt tataaacata
    42121 agattaccaa tatctataaa tggtgggctt gtgcatttag gaactgcaat gttgttcata
    42181 tcagcaatag tatttggaag taaaaaaggt gctttatcag gagctattgg tatgtcactt
    42241 tttgatttga tatctgggtg gactttatgg gcaccgttta cctttataga tagaggagtt
    42301 atgggttatt tacttggaaa gattgcatgg gcaaatggta ataatgggaa taatttttta
    42361 atcaatgtaa taggaatatg tgtttcatca atttggatgc tatttggata ttatgtaaca
    42421 gaagttatac tttatggtaa ttttatagta cctcttactt caataccagg taatttaatg
    42481 caagtattga taggtcttat aatagcgctt cctatttcta aggtattgaa aaaatgtata
    42541 aaataaatat aaagtgaact aaaatttatg atagcataaa ttatactgta taatattagg
    42601 atggagtaca taattaaaaa attttagtat agaggttagt tcacaatgtt atcagttaca
    42661 agatattcaa tcaaaaatgt tcgtttaaag ccgtgggtaa agtttatttg gtattttgag
    42721 acaaaatcaa atatactgtt aaataataag ttacttccga cagacagtat agatattatt
    42781 ctcaatcttt ctgatgttat ggaatataaa attgaaaatc aagactatat tgctagtaat
    42841 attcatttta atggaataag ggataaacat ggatttatta ttcaacatgg aaatatacgt
    42901 gttataggga tttcttttta tccatttggt ctctatccat ttttaaagat accaatttca
    42961 gagtttaaca gacaaattgt tgacctagaa gcagtatcaa aactatttgc aaaaaaatta
    43021 gaagagtcat taaatccaac aaaagcaata gaaaaagttg ttttatgttt agaagaagta
    43081 ttattatcca tattagaaga agatttaatt tcaaataaat atgtaaagct tctaaattca
    43141 tttatatatg ttaatagata tagcaatata aaagcatttt gtgatgatac aaatataaat
    43201 attaaaacat tggaaagaat ctgtttaaaa tatacagggt atacacctaa gattttaaag
    43261 agaatttaca gatttaaaat ggcaagcaat caacttattt ataattataa agatgatgaa
    43321 ctttttgatt ttatttatga aaatgagtac tatgaccaag cacactttat aaaagaattt
    43381 aaaaaatttt cagggacgtc accaattaaa tttagtggtg agaataagac gattaaagaa
    43441 aatataaagt acagctattt ataattatac tgtcgatttt ttacaatacc tccaatttat
    43501 tttttgatat gatataggaa aataaattgg aggttttaca tatgaaaatt attacagaaa
    43561 tagttgagtt taatgtggaa gaaaatttat caaaagaaga gtttatagaa attgtaaatg
    43621 acttagaatt aaaatttcat tctattcaat ctggttttat tgatactgaa cttttatatg
    43681 atgaaaagtc tcaaaagtgg attatgatac aacattggga atctatagaa gacctcaaga
    43741 gtgtttctag taaaatgttt aaagattctt caactgagaa atttagaaaa gctttaaaga
    43801 atcaaactgt taagatgaat ataataccac aaattaaaag ttggaaatta tagaactgaa
    43861 tgtaaaagat agttaaatag tatcaaacta aattagccaa ataacatttt gtatttaaat
    43921 tgttatttgg cttttagtgt ttattttaat atgagtgtta tatactagaa aaaccaacaa
    43981 actgttagag tgtggattat cataatgtca tattcgggag ctaaaaggct taatactgat
    44041 ttgccaattg gtcaatccat gcatgtccat ctggatatgc tgttattaaa gcatttacaa
    44101 gccaatccct ttgctcttgt gataatagtg ggactgttac atcgaagtct ttttgataac
    44161 caagcctatc tatgtatatt gatttgcaaa acaaaaccac ttcgggagcc atatatggga
    44221 tattatctga tgttatcagt ctggccttat ccatatctct tataattctt tgattttggg
    44281 aaaaaagaaa ttttccatct ttctgtggat tatatatcaa ttcaataaaa tcgaactgta
    44341 actgttcgtc acactgtata ttcattttat agttatcatc ctcgactttt tttaattcaa
    44401 taaatgagca atcaggctta atcgcaaaaa cacaaatttg ctctatatgt tgcttgttaa
    44461 ggtcgataat aggtcgtaat aaataattac ccaagggctc atatatatcc catccttgat
    44521 taagcatata ctgaatgctc ttttctttat ctgtgtcaga aatccatatg tcaatatcac
    44581 tgtgtgggcg aatatgcata ttcgcaaata attctagggc atatccacca catattgaat
    44641 atgaaaagcc acaatcatta aaaaagttta cacaatcttt aattagtttg ttcatagcaa
    44701 cctccttttg catgtactga aataaattaa attttttaaa ataccaacta aatgtttgtt
    44761 ttgtgtaaat ataaaactgc cgatattaag gcagttttat atttactatt tagaaaattt
    44821 attaacaaat ttttgatatt cacacattat cttatcattc ataaaagaaa tacccataaa
    44881 agcagcttgc tcaccaaaca aagtaaaacg ttctatcatc tgttgtggat tacctggata
    44941 aactgttgga atacaccatg tgtttagaag aaaaccaaac atttgagcag caatctctgg
    45001 atttttaact tccatagaat tatcttgatt tccttcttca atcaatgctt ttatccaagg
    45061 cacaatatca tttataatct catcaaactg cgtagctaaa tacttaggat tacgcaatag
    45121 tgatgatgac attaaaaaaa tgtcttgttt attgtgctgt tcataagcat gaagcaacct
    45181 atattgtaat ttttctaatg cggtctttcc ttcatcattt tttacctcat acccataaaa
    45241 tccatcatta tacaaccgac ttgtgattgc cttaattatt tcgtcttttg atttaaagtg
    45301 atgataaata gcacctcttg aaaggccatc taaattatct acaatgtctt gaatcgttgt
    45361 ctgctcatac cctttttcta agaataactt atgtgaaata tcaagtattt tttgtatagt
    45421 attatcaggt tctttttttc ttcccaaaaa actcactcct cgaaacaatc atttggtatg
    45481 tttatcttag caaatacata atgatttgtc aatgtgtctt tttagcatgt tgaacaggta
    45541 tattgccatg ctttcattat tttatttttt tgccttatac tggactatat ttgcagtttc
    45601 ctaatatatt tggctcatat ttaaatacca ttatctaaaa gcactattgc tgtacgagag
    45661 gcaataggca ttttagaaga aggaggagtt gagctaaagt atacatcata atctcttaag
    45721 ttttcttttt ttgtgtttcc tccaaactct accatatcag tatttaatat cgttttgtag
    45781 tttgtatttt gaaactttga aatatcaact gattgtatta gattagggtg gaaattaaat
    45841 ataaataaat atttgccctt actgtacacc aaaatctttt tatcattatc catatgtata
    45901 agttcatgac tattttggat aaaaatatca ctgtattttg taagttcgag catagattta
    45961 tcaaaattta ataattgttt atactttaag ttgttatctt ctgaaaggct ccattgtctt
    46021 ctggcatact tatagctcca attgttgcct tctcgtggaa aatcaatcca ctcaggatgt
    46081 ccaaattcat ttcccataaa atttagatat ccttcacctg ctagagaaaa agttattaat
    46141 ttaattaatt taataagaga tattgctcta ttgataacat ggttatttga gtttatttcc
    46201 atattccagt acatttcctt gtcagctagc caaaaaataa ttgttttatc gccaactaat
    46261 gcttggtcat gggattcaca atatcctata tttttttctc caggtcgtct agtagtaagt
    46321 tcataccaca ttttgcccaa atcccagtct tcatcagata agttagagat agtttttatc
    46381 caaaaatcag gtactcccat tgcgagtcta tagtcaaatc caattccgcc gtcttttata
    46441 gggatacaca taccaggcat tccactcata tcttctgcaa tacttatcga attaggttta
    46501 atttctttca ttaattcatt tgcaaattgg agatatgtta tagcttcaat atctgtattc
    46561 atactaaaat atttttcata actatcaaat gaaactccaa gaccatggtt atgatataac
    46621 attgaagtta caccatcaaa cctaaagcca tcaaaatggt actcatttaa ccaaaatttt
    46681 atatttgaaa gcaaaaaatg tattacttca ggtttaccat agttaaaaag ttttgtaccc
    46741 caagcaggat gatttccctt tgaacctgag tggaaaaatt gatgttcact tccatcaaac
    46801 tcatttattc cttctaaagt attctttaca gcatgagaat gaactaaatc taataaaaca
    46861 gcaatcccca ttgaatgagc agtgtttatg aggtttttta agtcttcagg agtaccaaac
    46921 ctagatgaga ttgcataaaa gtttgaaact tggtatccaa aagatgcata atatggatgt
    46981 tccataatag ccataagctg aatggtattg tatccagctt tttttatctt aggaagtatt
    47041 ttttcagtaa attcattata agttccaata gattcgcttt cagtagacat acctatatga
    47101 cattcataga ttagtggaga agttatgttt tttaagtcaa aatcattgtc agtccagata
    47161 aatggagttt ttggttgcca tatttgtcca ttaaatccac caaaatcttt ttgaattact
    47221 ctttttatat ataatggtat acggtcaaat gtttttccat ttgcagtaac ttgtaccttt
    47281 acctctgatt tgtgaggtaa gctattttga ccaggaataa atatttccca attaccagac
    47341 ccaatttttc ttagaggatg agattttcta ttccaattat taaagtcacc aattagtgac
    47401 aaactatctg catttggagc ccattcccta tagaagaatc catcatttgt ctgatgaaat
    47461 ccatagtaaa gatgagcatt tgcaaaagaa ctaaaatctt tatagttttt caatgttttt
    47521 gatttcatat cctcataatg tttcattcgc ttttttatat cagattcaaa tggttgtagg
    47581 tatacatcaa tatctaaaat tttaaatctt ttttgacttt tcattgtatt tctcctccat
    47641 aataaatatt acatctaaaa aataatattt atctcatact
//
LOCUS       DAELLV010000006        47378 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.006, whole genome shotgun sequence.
ACCESSION   DAELLV010000006 DAELLV010000000
VERSION     DAELLV010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 47378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47378
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.006"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(111..665)
                     /locus_tag="KPD58_000302"
     CDS             complement(111..665)
                     /locus_tag="KPD58_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBG0264093.1"
                     /translation="MSIALILIDIQNDYFKNGKCELFQSEETAENAKKILLFFRKNNL
                     PVIHIQHISTDKTASFFLPNTHGSEINKIVFPLDNEEIITKHTPDSFFETDLQSILEK
                     KNITNLVICGMMSHMCIDTSVRTAKKLRYDITLISDACTTKNLSWNNIEINANTVHQV
                     FMASLQNSFADVKNTDKFLSNYHK"
     gene            complement(743..1108)
                     /locus_tag="KPD58_000303"
     CDS             complement(743..1108)
                     /locus_tag="KPD58_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0264094.1"
                     /translation="MKIRAEYTCLLEIVHDIIKGKWKTILIFELRQSNKTFSELQHNI
                     YGINQKMLIQQLKELRDFGIIDKISSAGYPLHVEYFLTNRGKKMLKAVEIMQEIGIEY
                     MLEHGQQEILDNKGICYNK"
     gene            1573..2358
                     /locus_tag="KPD58_000304"
     CDS             1573..2358
                     /locus_tag="KPD58_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0264095.1"
                     /translation="MIVKIDNTLEKEFWQYVSHEESLNLFIIGYVENYGFSSQYQDIW
                     SQVEDGNITSIILKNKSTLIIYSFKNNFNIGEMKNHIKDLDVESISGKKCVIDRLISK
                     YKDFYEKLDNKFCVLKEIKEIDFSNMKEYKIENAQEKDIDEIGKLLNRSDYKVSKNYI
                     EERKVHLKEGNVRAYFIRNDDTMISTVSTGMETSFLAMVVSVSTDKRYRGKGLASYMV
                     YNLSKELLLEGKVPCLFYNNDVAGKIYHNIGYKEINEWTILFK"
     gene            complement(2455..3279)
                     /gene="pdaA"
                     /locus_tag="KPD58_000305"
     CDS             complement(2455..3279)
                     /gene="pdaA"
                     /locus_tag="KPD58_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HBG0264096.1"
                     /translation="MKKDSLKKYIMIGAFALILFGIASINFKSLDKTKTQISSPTLDT
                     HEYDWYFNPREDGKQPSPIKEADFFKKYGAYYVGNPNEKVIYLSFDAGYESGNTPKLL
                     DTLKKHNAKAQFFVVESYIKSNPELIKRMEKEGHLVCNHSKSHPSMAGITDFEKFKEE
                     ITSVEKAYKDVTGKEMPKYFRPPMGKFSEQSLKYTQDLGYKSIFWSFAYVDWYEKKQP
                     THEFAKNKIYSRTHPGAIVLLHPNSSTNTEILDEVLTHWEKEGYKLKTLDYLNNKK"
     gene            3500..6619
                     /locus_tag="KPD58_000306"
     CDS             3500..6619
                     /locus_tag="KPD58_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBG0264097.1"
                     /translation="MDNIISVLNDVVFLDIEVSGLDCLNSEILEVGAVKVKDWKIYTY
                     ESLIKNKFEVPVEVFSVCKNLDKNDLEIANEIELVEDRLVNFVEDSFIICHDLSLKKK
                     FLEYHMPKLKNKFIDLIELAVILEPYHKDYSLEYLKNTLTNCNSKVENRALSDAIDII
                     NIVNCLLVKFNNYEKTTLEPLSFKINSYLKKFNLPTWEWSKFLEEANYDLSNNINIKK
                     EYNIFDSKEEKKKERETLKILNEEEKNYEELLKYKTIWENKEGFTYEYRPGQYELTKT
                     IRELFRNSEDEEKIACIEAPTGIGKSVGYLLPAILEARINKKRLLISTDTKELQIQLI
                     NKDIPNVLDSLGLNGKVSYGYIKGKNNYICIDRLEAYIDDYESQNPTKGEILSLIFLK
                     RLVEGGKYGDIEEINYLVFDNFKEISTHLRNVSCDPNMCRPKKCKKDCLYKNRIEELK
                     EEHITVVNHSLLAKWPYKDEKPLENIIVDEAHNLTEKGYDFFSSIINSKSLRYLLQEI
                     YPYEFIQNSSFIYKKYSRNMRKIKAFDKFYNVLKIGREDKQKIARSINLIIEEIDSIL
                     KFGNCNEYNNVSNYNLRWELNLQIDEIVGKLKKDGIDTEISYRAYSEKIKLSCEKIIK
                     NLVSIIIIIYRNIDDDSIDKEADIYKFGKAKTRDLEDIKIIFEIFLEYDEKDDYARIV
                     EIDKNYNDFEFRVVPLKIADLFEENILSQLEKGIFLSATLSLSESMSYFKNTLGIDRV
                     KNVEKIIEPIFDYKNRVSVVGFSDICEYRNSEFPNEMSKIISNISKITEGHTLALFNS
                     KDRQEKTYEILKKYLHSFNLEIYADKKGIRHLNDLNRKCVVLGSKGCFEGVDIPGDGL
                     VCVTLDKLPNLNPKDPLYFTIMKKYGIDYYTINYPQMTIKVKQAMGRILRSKYDYGCF
                     VIFDVGTNISVLKRLEKDLHDCKISKVNSNEFYTYIRRHLNKYRSLILKSVIFDTIKA
                     LNVDAKMDNNDVDKDIIKKDINENIRQRAVKGEVYHIDIIKKDMKVKYFDRNYLINLD
                     IFMREEDKN"
     gene            6637..7236
                     /locus_tag="KPD58_000307"
     CDS             6637..7236
                     /locus_tag="KPD58_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HBG0264098.1"
                     /translation="MAKQKFYAVKKGKNIGVYNTWDECKKQVNGFSGAEYKSFSTFQE
                     AKEYIDGSEKLSFQEDKEFIEAYVDGSYEHSVKMYGSGVVILKNNEVIKTYSEKGKEK
                     TLVSMRNVAGEIEASKIAMQYCIDNNVQNLILYFDYEGIEKWCTGVWKTNKEGTIAYK
                     NFYDSIKNKLNVKFTKVKAHSGNKYNEEADKLAKKAIGV"
     gene            complement(7493..9622)
                     /locus_tag="KPD58_000308"
     CDS             complement(7493..9622)
                     /locus_tag="KPD58_000308"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HBG0264099.1"
                     /translation="MPNLPSLGSKAPDFKANTTNGPIRLSDYKGNWIVLFSHPGDFTP
                     VCTTEFLCFAKYYDEFKKRNTELIGLSVDSNSSHLAWMYNISLLTGVEIPFPIIEDRD
                     MRIAKLYGMISKPMSDTSTVRSVFIIDNNQILRTILYYPLTTGRNIPEILRIVDALQT
                     SDRDNIVTPANWFPGMPVILPYPKNYKELKNRVNSCNKKYSCMDWYLCFVPDNYNDEE
                     VSKKIDNTCSWKKEHTKNIENECNCEHEHHDYLNKALDCKQEHKTDIKDDCNHEKKHT
                     KNTNKVHNSKQDKFKDKSCDEMNFNYDKDESCDKINSSYNKEDSSYEDFYKHNYKNYD
                     YTSEKNTKKIAMKTLKDSKKLVRPQITDPYNPIVENANCPDINPIVAEYVLGNPTNVD
                     AQLLDAVIFAFAEIDQSGNLFIPYPRFLNQLLALKGEKPSLKVIVAIGGWGAEGFSDA
                     ALTPTSRYNFARQVNQMINEYALDGIDIDWEYPGSSASGITSRPQDRENFTLLLTAIR
                     DVIGDDKWLSVAGTGDRGYINSSAEIDKIAPIIDYFNLMSYDFTAGETGPNGRKHQAN
                     LFDSDLSLPGYSVDAMVRNLENAGMPSEKILLGIPFYGRLGATITRTYDELRRDYINK
                     NGYEYRFDNTAQVPYLVKDGDFAMSYDDALSIFLKTQYVLRNCLGGVFSWTSTYDQAN
                     ILARTMSIGINDPEVLKEELEGIYGQF"
     gene            9888..10136
                     /locus_tag="KPD58_000309"
     CDS             9888..10136
                     /locus_tag="KPD58_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264100.1"
                     /translation="MMICFAMTFALVLINSVMGVHIGLDYYFLMGSVLTVCLTIGIVL
                     CNARKIFDSKAIHKTRTKTVSNNKKQKSHSRDIKRKIS"
     gene            10302..11693
                     /locus_tag="KPD58_000310"
     CDS             10302..11693
                     /locus_tag="KPD58_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HBG0264101.1"
                     /translation="MNFRDLFEKAVDFIDEKRKSIVAISLSTLGVIALIIVFFLSSNE
                     FSVGNEANELLKIIEKRQYSIAVDYYTSIEKKFSDSKMERFNKSVSKKINKLLLNSGD
                     KYLDGDISQESFIGLINTVKELNKISIDVDDLLAQADRVREMYKEENTTYDIAINYIS
                     TVSILNGMGSNLDVYKQNIETIKESRDVYDSAVKDQKAYKYYEAIEKYNKVLKEDEKY
                     YSMAQNGKEQCIEEMYDYYISRAEEANTSGDYERALQYIEYLKDDYSDDEKVQSLEKK
                     YKKNLSLYTLTSDDIINVIAKKSGKDKANLSINSLPQMIKNNKYYYAEVYEYDKLINE
                     VLVSAKTKDLYSYKDGKKDYKVDYGDGYFRILEDGSYQFGITKDKAKFVLTNTLDEKE
                     NKYKKIEILDIEKADRYVKSKKSLEELFGKYKNIYYYAVVNKGLFRGKEVYAINIYNE
                     KIYAISENGLNEY"
     gene            11808..13277
                     /locus_tag="KPD58_000311"
     CDS             11808..13277
                     /locus_tag="KPD58_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBG0264102.1"
                     /translation="MSNVNKKLVIAIGAVALVVIIFIGIMCMQFKGEKIAKNTYVNGV
                     NIGKLTKSQAKQELAKKYKLENVDFNYNDKSWKVKSKDLNLSTDLDKTVENAYNLNRK
                     SAFFGNLSKTISANFGKKSNLVVAINYDKNKLKAEMEKIAKEIDVDVKDATLDISGEK
                     VKVIPDSDGLKMDISKSMENFDNQTKKGNYKNELVVKATPAKVKKEQLANIDTNLGTY
                     STTFKTSQINRSINIKLATDNISNVLLMPGETFSFNKHTGKRSKENGYKSAPVIMEGE
                     MEEDYGGGVCQVSSTLYNSVLYAGLEIVNVKNHTIPSSYVPKGRDATVADSGIDFLFK
                     NNLKHPVYIKNYVSGNQIVCNIYGSAEDKQNITISTKLDGVSQTTMKRVNDPTMPKGK
                     EKVDKSGRNAYSVSTYRTFNDANGKKIKTEKIANSYYPKKEGIILVGTMEPKPEEKPN
                     TDENKNNQNTNNQNPNNQQKPETPPTDNKPNETQPQPQA"
     gene            13342..13935
                     /locus_tag="KPD58_000312"
     CDS             13342..13935
                     /locus_tag="KPD58_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HBG0264103.1"
                     /translation="MNLNNITNYVQSICENISNVLDVDVTLVTKDLTRIAGTGIFRGK
                     IGETISEKSVYSLVLKDGKSYVINKEIEENCNKCANRENCKELADICTPVKMGEHILG
                     ILGIAAFNEEQKEKILSKDKELTAFVRSMSDLISFKLDELYKQEVKTIDDLEREAIEN
                     AIKKYGSNTEGMKKVAEALNIGIATLYRKVKKFNIKS"
     gene            14117..15463
                     /locus_tag="KPD58_000313"
     CDS             14117..15463
                     /locus_tag="KPD58_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG0264104.1"
                     /translation="MPRKILDISLIEDNKSDLTFFNIEEEDGDETLKALKQSYVTTNY
                     KEETLLNLQGALNSKSDTTSKELKLESYKRYNNALDLAYKNYITSAINMVNKALEINP
                     KDIDILNLRGLLKLLKCDFAKSFESFYTALCYENNHISRKYVNLISSEDFKTFLGRYN
                     HSIRFINEELNYESIHILENIIEEEPELIEPYVILSLLYDKLGNVKKREGYLDKLKEL
                     DKDNPVFEKNEEEKEDTSKKEEIKKVKRKKSILPYIIVGCVLIGMGIYLIQSKKRIEN
                     LNNQISSKEEKLSETDKKLGETSKKLEKTNKELNEVKNKEPEREITVADEEDLYYQAL
                     NLKKNKEYEKAIDNFKSVVSSGKTKKYISESIYQLAITNKLLGNKDEAIKYYKKYINT
                     YTKNDQYYDDSYYELGMLYYDNGDLKNAQQTFYSLRSEVPDSMYNNSKIKEILSEK"
     gene            15645..16016
                     /locus_tag="KPD58_000314"
     CDS             15645..16016
                     /locus_tag="KPD58_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBG0264105.1"
                     /translation="MKVFNQLVIILSIWVIGEYISSFIQGIILIPGSIVGMLLLFLLL
                     QFKVLDLSSIENVSGFFLDNMAIFFIPAGVSLIKSLDLIRENVLVLLLVICLSTLIVM
                     YTTGIIVEKMIKKKEEGQKNV"
     gene            16009..16698
                     /locus_tag="KPD58_000315"
     CDS             16009..16698
                     /locus_tag="KPD58_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBG0264106.1"
                     /translation="MYNVLQTPVFGIIVTIVFFNLGRYIQRKTSNPICNPLLIAIVGI
                     ILFLSISKIPYENYKIGADSLNFFLGPVTVVLAVPLYKQFELFKKHMFEIIVGIGCGI
                     VISFVSVLIIGKIANSDVSIINSLIPKSITTPMGISLANSLNGVESITVVAIIFTGIF
                     GGMVASTVFKLGNINHPVAKGIALGTSAHALGTTKAFELGEIEGAMSGVSIGVSGTIT
                     VILIPIIMNFI"
     gene            17218..18084
                     /locus_tag="KPD58_000316"
     CDS             17218..18084
                     /locus_tag="KPD58_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG0264107.1"
                     /translation="MSEVKNTLQKHPVLRYSTMLFGMLMTSIGINGFLRPAHLLSGGA
                     TGIATSINYLTNINVGLLTFLINIPIFILGFIYLEKEFCISSLVNMIVFSLLLGATQE
                     ISNIIPIHDILLQSVYGGILSGLGVGVVFRTRSSQGGTDIIAAILKIKKNIEMKDTAL
                     AINGLIVLTGSFLFGLDLALYTLIGLFLNAYSMSFIKDAMNYQKSVMVMSNEVDLIAE
                     DIMKSLVRGVTFLDAEGAYTHQKKKIIYTIVSSNEIPKIKDIALKYDKKAFISVNDVT
                     EVKGRGFKAKDL"
     gene            18326..19135
                     /locus_tag="KPD58_000317"
     CDS             18326..19135
                     /locus_tag="KPD58_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264108.1"
                     /translation="MNLSKEYVNLYDNYLKYINEVNKEIRSIKNMKKRVLKRGDFAPE
                     NEVLELEGKTIRDIDDVDTKKPKNKIYKFSNGDVYVGKFLEGKMEGKGSYTFFVDEGT
                     VMEYIGEFKEDKKNGKGNFIFSNGNEYIGYFEEDMMSGIGNMLYSSKDEYIGTWKNGK
                     KDGKGIYKWSDGCIYAGEFKGGKMEGYGICYNSKGEVLYEGEWKNNLIHGKGTYIWEK
                     GKKYIGEFMHGKKHGQGTFYLNNELVYEGTWKFDKPSIFDRTLDEIFSLRL"
     gene            19279..20343
                     /locus_tag="KPD58_000318"
     CDS             19279..20343
                     /locus_tag="KPD58_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBG0264109.1"
                     /translation="MKRLIALVLVVLIVLFYSPELLKEGYDLKEKFFQSEIWNEFLDK
                     ININDNSKKDKKEKSQNLQSNNTEGENLGESDIKNFEKISLGDNLSYVLSSIGKPGRI
                     DISEYGFDWYVYNQYGKEFAMVGLENDEVVALYSNSINSCENQDIKLNQDRQTVRTKI
                     TPLKYKRKGNTRYIINSENQYDIISKEGKYITIFYDIHEENRVCSYLIIDKSTEDEFK
                     NLYPDDSEELKKCFELEVIDLVNSVRNQRGLNSLRYSEQATLSSRKHSEDMRDNNFFD
                     HVNKKNETPFDRMKREGIVYTSAGENIAAGQINAIYAHEAWMNSEGHRKNILGNYNNI
                     GVGVIFGGSYKTYYTQNFYK"
     gene            20371..21090
                     /locus_tag="KPD58_000319"
     CDS             20371..21090
                     /locus_tag="KPD58_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG0264110.1"
                     /translation="MTSWKKKTVYKCLIAVALFCGIVLISNFSKVSALMMDTNGNVLI
                     KHGSREKKLIAITFDDGPHPKETSQVLDVLKKYNVKATFFIAGKHAKWYKEPLVRASK
                     EGHEIGNHTFNHPDISNLSSSQIEEEIVKCEDILKEVTGKKPTLFRPPFGSYREKDLI
                     EIAKKHDYKVVLWTGVDVKDWKNPGANSIADKIINKVQNGDIILLHDYATNDTVEALD
                     MFIPKMIEKGFKFVTVSELIK"
     gene            21114..21698
                     /locus_tag="KPD58_000320"
     CDS             21114..21698
                     /locus_tag="KPD58_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HBG0264111.1"
                     /translation="MILMIDNYDSFVYNLVQYIEELGEAVVVKRNNEIKISDIEELNP
                     EVIVLSPGPCSPKEAGICIDIVEHFKGKKPILGICLGHQTIGHVFGGDIIKAQQPVHG
                     KVYSINHTNKGVFRGLKNPLNVTRYHSLIVDSNTVPKELEITAITDKGEIMGIRHKKY
                     LIEGVQFHPEAILSEYGHEMLKNFITEARERVHM"
     gene            21700..23049
                     /gene="pabB"
                     /locus_tag="KPD58_000321"
     CDS             21700..23049
                     /gene="pabB"
                     /locus_tag="KPD58_000321"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HBG0264112.1"
                     /translation="MIREINTKLNSFEIFTIFRNEHDSFILDSAMDKEKLGRYSFISS
                     QPFKVLKYKDTDENPLEVLKEELHKYRVVNDTNLPFVGGAVGYLSYDLGNYIENLPRT
                     AVDDIEMPDMYFGFYNHVIVIDHLVQKTYIATPNIDIELEEKIIDDIEQRILKEEKKG
                     IDSICYEEKEVTPIRLKSNFTKEEFKNAVQSVREYIRQGDIYQANLTQRFSGETELTS
                     FELYRDLRRFSPAPFGAFLNFEDAHILSNSPERFIRCVNKRIETRPIKGTRPRGKDKE
                     EDLRLQQELRNSEKDRAELLMIVDLERNDIGRISKTGSVKVPELFVIEPYANVNHLVS
                     TVVGELKDDKDATDVIKATFPGGSITGAPKIRAMEIIDELEPTQRNVYTGSIGYIGFN
                     GDMDFNIAIRTIIKNDKKVYFQVGGGMTWDSDPDEEYQETLDKAKSIMKALRGYYEE"
     gene            23039..23782
                     /locus_tag="KPD58_000322"
     CDS             23039..23782
                     /locus_tag="KPD58_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HBG0264113.1"
                     /translation="MKNNVGFDSSLSKFGIGLFETIRVKNGIAVDLDIHIERMLSSIN
                     CLDLDINYKKDFLINEIVTYIKKENVINKALRITVFDEGYNISIRDIPYNQETYEKGF
                     KLAISPIVRGNSLIHRHKTTNYFENIYTKNIANKTGYNDGIFLNSEGVILECSMSNIF
                     FIKGERVYTPSDRLPILNGIIKKRIIEICDELHIELIENEINISEISSFDFVFVTNSL
                     MGAIKVTEIDKIKFNKENVVFDKIVKLLE"
     gene            23816..24517
                     /locus_tag="KPD58_000323"
     CDS             23816..24517
                     /locus_tag="KPD58_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264114.1"
                     /translation="MIGCIYEVYNEEMTKIIENQNTLAVYLVGSSKDVDFTLYDVVIN
                     DIDVFVFVKEGEKQERILFKKKGIEFDVNYFSKDGVKKLLSNREYFFVKEMKDAKVLF
                     DRENISHIIKDISRDIYLEGPSKMSLEEKSFIKQDIGAKILNLKNKEKFDVFEYHFLT
                     NLYLKDIIIGYFNINDKWVPKDKKLLKVLKKENNELFELVSKVSENYDYQRLLDVYNY
                     IFKEIETKEVIKLIF"
     gene            24543..25115
                     /gene="folE"
                     /locus_tag="KPD58_000324"
     CDS             24543..25115
                     /gene="folE"
                     /locus_tag="KPD58_000324"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HBG0264115.1"
                     /translation="MNKVDKEKIQHAVREILEAIGEDPDREGLIETPNRVARMYEEIF
                     SGLSEEPRDHLKVLFADEKHEELVLVKDIPFYSCCEHHLVPFFGKAHIAYLPKGGRLT
                     GLSKLARVIDTLAKRPQLQERITKNAADIIMEELQPYGVLVVVEAEHMCMTMRGVKKP
                     GSKTVTSAVRGIFEKDIASRAEAMSLITMK"
     gene            25179..25970
                     /gene="folP"
                     /locus_tag="KPD58_000325"
     CDS             25179..25970
                     /gene="folP"
                     /locus_tag="KPD58_000325"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBG0264116.1"
                     /translation="MFNYGKRTYIMGILNVTPDSFSDGGDFNNLDIAIQHAKDMVDQG
                     ADIIDLGGESTRPGHSYVDSDEELRRVIPVIKKLKQELDIPISIDTYKADVAEEALKL
                     GVTMVNDVWGLRKDKNMASVIGKYDAEVCIMHNQDGTNYDKDIMESIKDFFKVSIEMA
                     MSCGVKKEKIVLDPGVGFGKDFEQNIEVLRRLNELKDLGYPILLGTSRKSVIGKVLPV
                     EPKKRLEGTIATTVLGIRDGVDIVRVHDVYENLMAARMTDAIYRK"
     gene            25981..26346
                     /gene="folB"
                     /locus_tag="KPD58_000326"
     CDS             25981..26346
                     /gene="folB"
                     /locus_tag="KPD58_000326"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HBG0264117.1"
                     /translation="MDKILLSNLGFYGYHGVLKEENFLGQKFFVDMELYIDSREAGLS
                     DDINKSVSYAEVYNVVKDITENKQFNLLEALAENIAEEVLNKFILINGVMVRVRKPEA
                     PVNGIYDYFGVEIRRTRDE"
     gene            26339..26845
                     /gene="folK"
                     /locus_tag="KPD58_000327"
     CDS             26339..26845
                     /gene="folK"
                     /locus_tag="KPD58_000327"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HBG0264118.1"
                     /translation="MNKAYLGIGTNMGDRFDNLSRACELLKNSDSIYKVKESSLYETK
                     PWGYTEQADFLNMCVEIETEFEPYELLEYCQEIERELHRERIVHWGPRTIDVDVLFFN
                     DVVSTDERLTIPHPRIQDRAFVLIPLMDLNEELIINEKTIKEHLNLLSAEEREEVKEL
                     VGYERKPI"
     gene            26826..27611
                     /gene="aroF"
                     /locus_tag="KPD58_000328"
     CDS             26826..27611
                     /gene="aroF"
                     /locus_tag="KPD58_000328"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBG0264119.1"
                     /translation="MKENLFNNNSKIEINTNNKKITVKKDKLIIAGPCAIESYEQLLE
                     TAKFVKSQGANILRGGAYKPRTSPNSFQGLKKEGLEILKAVKDEVGMAVITELMDVRD
                     MDELYSISDIIQIGSRNMQNFTLLSEVGKQNKPVMLKRGIASTITEWIGASEYIAIEG
                     NSNIIMCERGIRTYNDYTRNTLDLAAVPIIQKETGLPVVVDPSHATGVRYLVKPMSLA
                     SFACGADGIMVEVHPDPENALSDGAQSLCFNEFEDLMKSINNY"
     gene            27866..29656
                     /locus_tag="KPD58_000329"
     CDS             27866..29656
                     /locus_tag="KPD58_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBG0264120.1"
                     /translation="MNDIKDIIEEIKARCDIASIISEYMNIKQSGANYKGLCPFHGEK
                     TPSFYINTSKQIYKCFGCGEGGDVINFVMKMENLDFMDAVKILANKCGIEINTNMNEE
                     TRIKIEKSKKFQDIHTEAARFYLSNLLGSKNLGYEYLRIRGLDDKIIKKFGLGFSLDS
                     WNSLMNTLISKGYKKQDLLECGLIAKNRDGTNCYDKFRNRVMFPIFDYRGNIIGFGGR
                     VLDDSLPKYLNSPDTLIFNKKQNLYGLNFARKNLESKTIVLVEGYMDLISLYQYGIKN
                     VVATLGTALTEQQGILIKRYADTAIISYDSDEAGIKATLRAIDILTKLGINVKVLDLK
                     DAKDPDEFVRKYGLSDYKKAMDVSTHYIKYKIDHLKKEFNIQKDEERVKFAKEASKII
                     KQLTSPVEIDFYTKYLSNQIDINVESIKREVYGKNYNKPYNNKNQKKIEEKVIEKVEV
                     RQDGKQLVEETLIKIMLEDKKIREIALLKVEESDFLLKESKEILNYMIKNQELDKITI
                     DKLKSLNISEEYLKELNSISLNSINLENTKEIEGIITNIRKNSLEEQINSLLREQQEL
                     ENNNDMKEVDGRVMEIALKIVEINKILKSL"
     gene            29674..30840
                     /gene="rpoD"
                     /locus_tag="KPD58_000330"
     CDS             29674..30840
                     /gene="rpoD"
                     /locus_tag="KPD58_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBG0264121.1"
                     /translation="MENKSNKKELKKVTAKTLIEKGKKQGSLTLAEIMEAFSETELDK
                     DQVENLYETLGNLGIEITETKNYKADIDFSVADDDLSIGHLDEDAEAISHDDSSAIEI
                     ETVDLSLPKGISIDDPVRMYLKEIGKIPLLKPHEEVEFARRMHEGDEIAKQRLVEANL
                     RLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKFDYTKGYKFSTYATWWIRQAITRA
                     IADQARTIRIPVHMVETINKLIRVSRQLLQELGRDPKPEEIAKEMEMTEDKVREIMKI
                     AQDPVSLETPIGEEEDSHLGDFIPDDDAPAPAEAAAYSLLKEQIEDVLGSLNDREQKV
                     LKLRFGLEDGRARTLEEVGKEFDVTRERIRQIEAKALRKLRHPSRSKKLRDYLD"
     gene            31005..31694
                     /locus_tag="KPD58_000331"
     CDS             31005..31694
                     /locus_tag="KPD58_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBG0264122.1"
                     /translation="MKLTDRLLKIASLVSDGKKIADIGTDHGYIPVYLLKEGKVPFAV
                     LADVNKGPLDNAHKEVIQNNLLDKVDLRLGSGIEILEIGEVEEVIIAGMGGILISELL
                     EAKKEVAHNVEKLILQPMQAQEELRYYLLNNGYEILEEVLVREDFRIYEIIVAKYTGK
                     NTIIEDEIYYEVGIKLLENKDSLFNDFIEKKIKTYSSIVNKLEGKNGEAIDKKRKESE
                     VAIKKLENLIK"
     gene            31708..32805
                     /locus_tag="KPD58_000332"
     CDS             31708..32805
                     /locus_tag="KPD58_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBG0264123.1"
                     /translation="MLLKSLTRKIEKKYPLNLAEDWDNVGLIVGDFDMDVKKVLVSLE
                     ANEDVIDEAISKNIDLIVTHHPFIFGKINKINSGDLKGRLIQKLIKNDISLYSMHTNF
                     DIAFDGLNDYFMEIMEFGNSKVLDITKSENLYKLAVYVPHNYSDELRKVLSNSGAGHI
                     GNYSDCTFSIEGEGQFKPLEGSNPFLGSVNDIETVNEVKIETVVPQKLLGGVISSMLD
                     AHPYEEVAYDLYKLENKGEIFGLGRISKLDKSMTLESLSRKIKEKLNMKHIRVVGNLS
                     TDITKVAVVTGAGSEFVKKAKRQGAEVLITGDVKYHEAQDALDIGMCIVDCGHFDTED
                     IFKNVMKRFLDEFSEIEVIKSNVYLNPFSTI"
     gene            32868..33440
                     /locus_tag="KPD58_000333"
     CDS             32868..33440
                     /locus_tag="KPD58_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBG0264124.1"
                     /translation="MKVAVIFHSVCGSTYLLAREYKEALEEMNIEVDIFRVSDEVAKT
                     LPQYYLINSKEYKDEFESINVIKSGKEILDYDAIFMGSPTYYGNVSGPMKMFMDSFSD
                     IWVGTPLSGKIFGCFATAGSQHGGGELALQAMNIFAQHMGMTLLSVPCSVRGGYPAYG
                     ILHIAGDNSDIRPNDDAKIGIRDYLKRLNI"
     gene            complement(33500..33967)
                     /locus_tag="KPD58_000334"
     CDS             complement(33500..33967)
                     /locus_tag="KPD58_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBG0264125.1"
                     /translation="MFKEMRLKKREMTKEDTVEVLKNGEFGTFSTISENGYPYGVAVN
                     YVYFNDSIYFHCARNGHKLDNISKNNKVSFLVVANESVIPDKFSTTYSSAIVFGKACT
                     VENEEKKNALVEIIKKYSKGFFEEGMKYIEKDMNLTTVVKIEIDHISGKASRL"
     gene            complement(34353..34829)
                     /locus_tag="KPD58_000335"
     CDS             complement(34353..34829)
                     /locus_tag="KPD58_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBG0264126.1"
                     /translation="MLSKAEIKYFNECASEILSSEKVQLMRTFPHHGNVSCLEHSLSV
                     AYYSYLLCKKLHLSVDIQSVIRGALLHDFFLYDWHYKGDRKGLHGFTHPREALKNATL
                     FFQINEKETDIILKHMWPLTVKPPRYKEAFIVCLLDKFCCLVETLKIHSLLSPYHV"
     gene            complement(34949..36040)
                     /locus_tag="KPD58_000336"
     CDS             complement(34949..36040)
                     /locus_tag="KPD58_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HBG0264127.1"
                     /translation="MSKIECLFTIFPVLILMYATFYFMPYFVGKKHIYGVSIDQEHKN
                     YNYFIKLDKEFKKLLSLGFIIDFILVFILVFTFNKLELSYFIGIIGFLLYESILYIHT
                     HKKAKKLKSEIYSKLGHIDIDSKLIVDMDFINKKNKIIKKFKIIHLIPILFTLGMSIF
                     IAFNYNQLPDSIATHWNINGSPDVFIDKSFLNVFKLIGTDFCLMVLLYITSIGSIKSR
                     IKIDTNRIEESRVENIKYLNKIGYLFLILMIIMTTQFFTTLLSIKTKLSTAMNITTLL
                     VIIYLIATYINSPNLKFNSSYSPEEDEKYWIAGIMYNNPNDPSLMVNKRFGIGWTINF
                     GNPLGKILYIAIALLLIFSLFSLIKSLLL"
     gene            complement(36033..36422)
                     /locus_tag="KPD58_000337"
     CDS             complement(36033..36422)
                     /locus_tag="KPD58_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0264128.1"
                     /translation="MVLNLDFNSDKSIYVQIKEEITKAIASGELKINESLPSVRSMAE
                     NIGVNLHTVNKSYNELKEEGFLNIDRRKGAIVAQLPMKIDNTTRQKNKLDLELLVAKS
                     YLSGISKEEFLSLSSSLFDKYEVKYYE"
     gene            36614..37060
                     /locus_tag="KPD58_000338"
     CDS             36614..37060
                     /locus_tag="KPD58_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264129.1"
                     /translation="MLTYLMKRQMHKKDDGMSKPMMITGAVITGVGAYAVYKMVKSHK
                     SNSQMVNTNYYGNSDYDDYKYDEFDYDCDCRDTKQDYEYEELKKKVKEFNSRRINCEN
                     CPHVSQEEMMQYVNSVKDVKEEIDLHEDAKKDNIYKEDEYKKYEEE"
     gene            37324..38049
                     /locus_tag="KPD58_000339"
     CDS             37324..38049
                     /locus_tag="KPD58_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0264130.1"
                     /translation="MKRKEIVMLKLLIVEDDSTISFGIKYALEQEGFSIDISKDLSSG
                     KEMISSNEYSMILLDVTLPDGTGYELCQYIRGFSQVPIIFLTACDEEVNIVMGLDIGG
                     DDYITKPFRIRELISRIKAVLRRKGNTSEENKKILKFGDLSIYTLEARVYKNDKEIFL
                     TSVEYKLLLILIQNKNTVLSRTQILEKLWDVTYDFVNDNTLTVYIKRLREKIGDDLCE
                     PIYIETVRGIGYKWIGSENSVSI"
     gene            38036..39280
                     /locus_tag="KPD58_000340"
     CDS             38036..39280
                     /locus_tag="KPD58_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0264131.1"
                     /translation="MFLYNPEVKKFLSKYVTLMFIVIIISIGFSIINVSLTKEMVVRN
                     NQAIIGTLSSKYPNLESQIVDIITQGKSMENIDYGQKILIKYNYDKSIRINSEPIISK
                     LVLETIKINIILVCIIFILIFVLVVRYFKSIYNDLSDMTKYVYYSSEGKPFDMKNKNQ
                     EGQIGLLKTELLKMTTILNEKVELLKREKIFLNNTISDISHQLKTPMTSLIMLNDLLY
                     NDVPYEVKIDFLNKIKNQLNRMDWLIKSMLKLSKVEAKVINFKKDKVKFSELIHRAML
                     SMKIPMEIKNQKLTIEGNDNVSYIGDIDWSVEALVNIIKNCVEHTPEFGNIIITYKEN
                     PLFSELIIKDEGEGIDKKDIPHVFKRFYRGRSSSKEDSVGIGLAMSKSIIESQNGDIY
                     VNSEKGQGTEFHIIFHKMYDSD"
     gene            39360..40037
                     /locus_tag="KPD58_000341"
     CDS             39360..40037
                     /locus_tag="KPD58_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0264132.1"
                     /translation="MEILRVENLTKSYGKNETKVDAIKNVSLSVEKGTFIAITGPSGS
                     GKSTLLHLLGGVDRPTSGKVYINDVDIYNLKTKDLAIFRRRNIGLIYQFYNLIPVLNV
                     KENILLPAELDNRDIDREYFDDLMKTLGLIDREKHLPNQLSGGQQQRTSIGRALINRP
                     SIILADEPTGNLDSKNSKEILELLKLSVKKYNQTLIMITHDKNMALQADRIISIEDGI
                     IKSDEVM"
     gene            40038..42599
                     /locus_tag="KPD58_000342"
     CDS             40038..42599
                     /locus_tag="KPD58_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0264133.1"
                     /translation="MNLYTLLTIRYLKQNKRRTIVTIIGIILSTALICGIGNIFESFM
                     DYQMRETIKNDGSFHVTFYDVNRKNVEYVTKSAEIEKHAFTKQLGYSKLENSENAILS
                     IKQYDKNAINGYKVSIKEGRFPAKEGEIVLSESIINLIDDKLKIGDKITLKVGDMFDS
                     SKKKIDSMTFHEGDYIANEKDRTFKVVGIIEKPGFERYNGIATAIAYFNPMNNYNDTI
                     NVSVAVRNPKDVYKISNKISKNIYSNKDKEAVSDMIKYNEHLLRLQGASKYSNINNSI
                     KSIIIAVTILVIICTIATVYNSFSISINERKKQFGILNSIGATSSQIKRLVFIEGIII
                     SLIGIPIGLISGTVAIDLLFKIINKYFTESVVTKMSLQIVYNPIITIVSIIIVLLTIF
                     ISILLPAISASNISHLNVIKNTGEYKVGKIKSSRLIKMIFKTEGVLAYKNIRRNRKKF
                     IITLFSLMVSIIIFISFSGFTLLLLRGEEIRNSQRKYDLYLTAKNTARSVEDTISELE
                     DIDGIKNFELATGQYVSIRVSENKINKSKEDLIRKYYQKYKIGDSYEYDFSNNELIFP
                     GDFAVKNKINNLVQGSFNKERAIEENGVILVRKSAFEENGKKGVVELTNYKVGDTVNC
                     EYLDENALSKKVKVKILAITDEERLGLGYQNMGLQFITYDEVAKNLNLKLSKSLIFID
                     SGGSIKTKKEIEALANKNNFNFYDESASNESEKQDLKVIKIFVYGFIVVISLVSVTNI
                     LNTVSTSINLRKRELAVIQSIGVTPKGFRKMIYLESFIYGILSLLFGIPISIGIILIM
                     NKLISGVIEFSPIIPWTAIVICIVSVFIITFIAGYIPMSKLNKENIIDNIRRESI"
     gene            42820..44094
                     /locus_tag="KPD58_000343"
     CDS             42820..44094
                     /locus_tag="KPD58_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG0264134.1"
                     /translation="MITISHITAKNMYKSLEERINKFPQGAPPSDTLYKILNVLYTEQ
                     EAKLVAQLPIKPFRVKTAAKIWSVSESEAYRVLDKLASKALILDIEDNKGKKYIMPPP
                     MAGFFEFAMMRTRHDIDQKLLAELYYQYMNVEEDFIKDLFYSTETKLGRVYVQEEVLT
                     NDNEVSILDYERATHIIDESTHIGISMCYCRHRMQHVGKACDAPMDICMTFDNVANSL
                     INNKFARRVDKIECKELLHQAYEHNLVQCGENVRKGVTFICNCCGCCCEAMVAAKRFG
                     NLHPVQTTSFIPNINHENCVKCGKCITACPIDAISKVKEDGKEYIKIDEDRCLGCGVC
                     VRNCHKNSIMLLKRDEKIITPANSVHRAVLMAIEKGQLQNLIFDNNALASHRAMGAIL
                     SAILKLEPAKKILASKQLKSVYLDKLLSMNDK"
     gene            complement(44255..47296)
                     /locus_tag="KPD58_000344"
     CDS             complement(44255..47296)
                     /locus_tag="KPD58_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp20"
                     /protein_id="HBG0264135.1"
                     /translation="MFKQRLSKLLSSTLVLSMLFTAAPNITFADNTKDNSEKYQSSDI
                     ELHDYSKNAESYTKTKALAKEKIQTLLSKYGAVSAQYALIDNGKIEISGNGGVYSKQD
                     NKNLNKDNMYSIASISKMFTTTAVMKLVDDGKLNLDTPVVKYIPEFKMADDRYKEITP
                     RMLLNHSSGLMGSSFKNTILLADNDSYGHDNFLKELQKQRLKAKPGAFSVYCNDGFTL
                     AEILVERVSGMSFTNFLDKYINNPLNLQNTKTPENSFDSSKLAKAYVPYWEDAVPQDN
                     LNAIGAGGLYSSAENLCTFAQTFMKNSNGILSPASVKAMENKEYLNGLWPEGEDSILG
                     YGLGWDCVNTYPFNQYNLKALTKGGDSLLFHSNLIVLPDENMAVAVLSSGGSSQLNEI
                     IGQEILLSALKEKGKIKEIKPDKTFSKPQQVKMPSSLKENSGLYASSNMIKVDVNDNG
                     TLTVSSPYIENGPEDKYVYIGQDRFVSEKGNSCLKFVKEKNNITYLNMSSYDDVPGLG
                     QTASLYYVAQKVDDNNISNSVKEVWKKRSGKGYYLVDEKYTSQSYMFGSVKASFSLSD
                     ETPGYIVNTKIMDENNSNAFIEIPGVIGRDLSDIKLHKENGTEYLSFGTLTYVSEDSI
                     TNLPAEKSFTCELESNGYAKWYKIGDDIANKKIEVNLPQNSAFAVYDDKGVPVNYSLV
                     TKNNRVRLPKGGVIVFLGSPNARFEVTYQDEVNASALTGTDRYETSIKISQAGWENAE
                     NAVLINDSAIADALAATPFAYKKNAPILLTGSSQINEKTLAELKRLKVKNVYVVGGET
                     SINEKSLDTIKSNNISVSRISGSDRYQTSMNIAKELNNISNISKISVVNGEKGLADAV
                     SIGAVSAQNDMPIILTNENSNITEINNLFKNKKIDKSYVIGGEYTVSKNIESKLQNPQ
                     RISGSTRNETNAKVIKEFYKDSKIDNLYVAKNGMNKQDDLIDGLSVGVLAGKTKSPVM
                     LVGNSLDYNQKELFKTMRFKSVTQIGGNGNENSFKQIKEIA"
BASE COUNT        18959 a         5015 c         7912 g        15492 t
ORIGIN      
        1 tgtataaacg tatattatgt atataaaaat gatagaaagc atctaatttt aaatgattaa
       61 agagcaagct taaataaatc aaacttgctc ttttaacata gtgtatgttt ttatttgtga
      121 tagttagata aaaatttatc tgtgtttttt acatctgcaa aactgttctg tagtgatgcc
      181 atgaatactt gatgtacagt atttgcatta atttctatat tattccaaga taaattcttc
      241 gttgtacatg catcgcttat tagagttatg tcgtatcgaa gtttctttgc tgttcttaca
      301 cttgtatcaa tacacatatg gctcatcatt ccacagatta ctagattagt aatatttttt
      361 ttctcaagga tactttgcaa atctgtttca aagaaactat ctggtgtgtg tttagttata
      421 atttcttcat tatctagtgg aaaaacaatt ttatttattt ctgaaccatg agtattagga
      481 agaaaaaaag aagctgtttt atctgttgat atatgctgta tatgaataac tggtaaattg
      541 ttttttctaa aaaacagtag tattttttta gcattttcag cagtttcttc tgattgaaat
      601 aattcacatt ttccattttt aaaataatca ttttgaatat caatcaatat taaagctata
      661 gacatgatta tacctcctat ttatttttat tataatacta ttgtaaaaaa tgtaaattac
      721 ttactttttg gtatgtatag gattacttat tgtagcaaat tcctttatta tctagaattt
      781 cttgttgacc atgttcaagc atatattcga ttcctatttc ttgcattatt tccactgctt
      841 ttaacatttt tttaccacga ttagtaagaa aatattcaac atgtaatgga tatccagcag
      901 aacttatttt atcaataatc ccaaaatcac gtaactcttt taattgttga atcaacattt
      961 tttgatttat tccataaata ttatgttgca attcagaaaa tgttttatta gattgtctta
     1021 attcaaatat aaggatagtt ttccattttc ctttgattat gtcatgtaca atttcaagaa
     1081 gacatgtata ttcagctctt attttcatag catatcaccc ccagtaaaat gataataaat
     1141 tatatcactt gtcttgtgat atgacaaata tatatgaact gtatataaat tttatgtttt
     1201 attaagttct aaatttcttc tattgtagtt catatttagt acataaataa ttagtaagat
     1261 ataagtaaat aaatatataa catgactata gaaatttaac tttaaaataa cttatgataa
     1321 tttagaggag gaatatatat gaatatatca agaaaagcta tgaaaataat agaattagca
     1381 caaaaaattg caaataagag aggtatttca gtagaagaag cttggagtga agcagtaaca
     1441 gaatataaaa ataaatacga acatattgca tagatattta aaaatttaga atatttggta
     1501 gaatataatg attttaaatg atactataaa ttataaggta tatcattatt aacatagaag
     1561 gaggatatct gcttgattgt aaaaatagat aatactttag aaaaagaatt ttggcaatat
     1621 gtatcacatg aagaaagttt aaatttattc ataattggat acgttgagaa ttatggattt
     1681 agctctcaat atcaagatat atggtctcag gtagaagatg gaaatataac ctctataata
     1741 ttaaaaaata agtcaacact tataatttat agttttaaaa ataattttaa tataggagaa
     1801 atgaagaatc atataaaaga tttagatgta gaaagcataa gtggaaaaaa atgtgtaata
     1861 gatagactaa taagtaaata caaagatttt tatgaaaaac tagataataa attttgtgta
     1921 ttaaaagaaa taaaagaaat agatttttcc aacatgaagg agtataaaat agaaaatgct
     1981 caggaaaaag atattgatga aataggaaaa cttttaaata ggtcagacta taaagtaagt
     2041 aaaaattaca tagaagaaag aaaagttcat ttaaaagagg gaaatgtaag agcatatttt
     2101 attagaaatg atgatactat gattagcact gtgtcaactg gtatggaaac tagttttttg
     2161 gcaatggtgg tctctgtaag tacagataaa agatataggg gaaaaggact tgcaagttat
     2221 atggtttaca atttgagtaa agaattgttg ttagaaggaa aagttccatg tcttttttat
     2281 aataatgatg tagcaggtaa aatatatcat aatataggat acaaagagat aaatgaatgg
     2341 acaattcttt ttaaatagct ataaaaaata aaaaactcta tcgagaatta aagtatttat
     2401 aaataactat atttttacta taattcatga tagagttttt ttaaatttat gatactattt
     2461 tttattattt aaataatcaa gtgttttaag tttataacct tctttttccc aatgtgtaag
     2521 aacctcatct aatatctcag tatttgttga tgaatttgga tgcagtaaaa ctattgcacc
     2581 aggatgagtt cttgaataaa ttttgttttt ggcaaattca tgagtaggtt gtttcttttc
     2641 ataccaatct acataagcaa aactccaaaa tattgattta tatcctaaat cttgtgtata
     2701 ttttaaagat tgttcgctaa atttacccat tggaggtcta aaatacttag gcatttcttt
     2761 tccagtaaca tctttgtatg ctttttctac acttgtaatt tcttctttga atttttcaaa
     2821 atctgtaata cctgccattg atggatggct ttttgaatga ttacaaacta aatgtccttc
     2881 tttttccata cgctttatta actctggatt gctttttata taactttcaa ctacgaaaaa
     2941 ttgagcttta gcattgtgtt ttttaagcgt atctaataat tttggagtgt ttccactttc
     3001 atatccagca tcaaatgata gatatataac tttttcattg ggatttccta cataataagc
     3061 tccgtatttt ttaaaaaaat cagcttcttt tataggtgat ggctgtttac catcctccct
     3121 agggttaaaa taccaatcat attcatgtgt gtcgagtgtt ggactagaaa tttgagtttt
     3181 tgttttatct aatgatttaa aatttatact agcaattcca aaaagtatta gagcaaatgc
     3241 tcctatcata atgtattttt ttaggctatc ttttttcaca atttcacctc ttatttaaaa
     3301 tttaaagtat atttcttata atatagtttg aatatttata gttaaaaata ttcacatata
     3361 caaataataa atgaaagaag tttactgtaa gatataaagt tgatattaat tatcttaaat
     3421 aaagtgtata taaatgataa aatataaaga gaaatgattg taaataagtt aggctggttt
     3481 aacttgaagg gatataaaaa tggataatat aataagtgta ttgaatgatg ttgttttttt
     3541 agatatagaa gttagtggat tagattgttt aaattcagaa attttagaag ttggagctgt
     3601 caaggttaaa gactggaaaa tatataccta tgaatcatta ataaaaaaca aatttgaggt
     3661 acctgtagaa gtattctcag tatgtaaaaa tttagataaa aatgatttag aaatagctaa
     3721 tgaaatagaa ctggtggaag atagacttgt aaattttgta gaagatagtt tcattatatg
     3781 tcatgattta agtctgaaaa aaaagtttct tgagtatcat atgccaaagt taaaaaacaa
     3841 atttattgat ttaattgaat tagcagttat tttagaacca tatcataaag attatagctt
     3901 agaatactta aaaaatactt taacaaattg taattctaaa gtggaaaata gagctttatc
     3961 agatgctatt gatataataa atattgtaaa ttgcctttta gtaaaattta ataactatga
     4021 aaaaactact ttggagccat tgagttttaa aataaattca tatcttaaaa aatttaatct
     4081 tccaacatgg gaatggagca aatttttaga agaggcaaat tatgatttat caaataatat
     4141 aaatataaaa aaagaatata atatatttga ctcaaaagaa gaaaagaaga aggaaagaga
     4201 aaccttaaaa attttaaatg aagaagaaaa aaattatgaa gaattattaa aatacaaaac
     4261 tatttgggag aataaagaag gatttacata tgaatataga cctggtcaat atgagcttac
     4321 taaaacaata agagagttgt ttagaaatag cgaagatgaa gaaaaaattg catgtataga
     4381 ggcaccaact ggtataggta aaagtgtagg atatttattg cctgcaatat tagaggctag
     4441 aattaataaa aaaagattat taatttctac tgacacaaag gaattgcaga tacaattaat
     4501 aaataaagat attccaaatg tgttagattc tttgggtcta aatggaaaag taagttacgg
     4561 atatataaag ggaaaaaata actatatttg tatagataga ctggaagcat atatagatga
     4621 ttatgagtct caaaacccta ctaaaggaga aattttatct ttaatatttt tgaagagatt
     4681 agttgaaggt ggcaagtatg gagatataga agaaataaat tatttagtgt ttgacaactt
     4741 caaggaaata agtactcact taagaaatgt aagttgtgac ccaaatatgt gcagacctaa
     4801 aaaatgcaaa aaagattgtt tatataaaaa cagaatagaa gaattaaaag aagaacatat
     4861 aacagtagta aatcattcac tattagctaa atggccatat aaggatgaaa aacctttaga
     4921 aaatataata gtagatgaag cacataattt gactgaaaaa ggatatgatt tcttttcaag
     4981 cattataaat tcaaagtctt taaggtattt attacaagaa atttatcctt acgagtttat
     5041 tcaaaatagt agctttatat ataaaaaata tagtagaaat atgagaaaaa taaaggcttt
     5101 tgataaattt tataatgtat taaaaattgg aagagaagat aaacaaaaaa ttgctagaag
     5161 tataaatctc attattgaag agatagattc aatattaaag tttggtaatt gtaatgaata
     5221 taataatgta agtaattata atttaagatg ggaactgaat ttgcaaatag acgaaattgt
     5281 gggaaaacta aaaaaggatg gtatagatac tgaaataagt tatagagcct atagtgaaaa
     5341 gataaagttg agttgtgaga aaataataaa aaatttagtt tcaataataa taataattta
     5401 taggaatatt gatgatgata gtatagataa agaagcagat atatataaat ttggaaaggc
     5461 aaagacaaga gatttagagg atataaagat tatatttgaa atctttttag agtatgatga
     5521 aaaggatgat tatgcaagga tagttgaaat tgataagaat tataatgact ttgaatttcg
     5581 agtagtgcca ttaaaaatag cagatttatt tgaagaaaat attttatctc aattagaaaa
     5641 aggaatattt ttatcagcaa ctttgagttt atctgaaagt atgtcttatt ttaaaaatac
     5701 attaggaata gacagagtta agaatgtgga aaagattata gaaccaattt ttgattataa
     5761 aaatagagtc tctgtagttg gattttctga tatttgtgag tacagaaatt ctgaatttcc
     5821 aaatgagatg agtaaaatta tatctaatat atcaaagatt acagaaggtc atacattagc
     5881 tctttttaat agtaaagata gacaagaaaa aacttatgaa atattgaaaa aatacttaca
     5941 tagttttaac ttagaaattt atgctgataa aaaggggatt agacacctta acgatttaaa
     6001 tagaaaatgt gtagtacttg gctcaaaagg atgttttgaa ggcgtagaca ttccagggga
     6061 tggtttggtt tgtgtaactt tagacaagct tccgaatcta aatccaaaag accctttgta
     6121 ttttactata atgaagaagt atggtataga ctactatact ataaattatc ctcaaatgac
     6181 cataaaggta aagcaagcta tggggagaat tttaagaagc aaatatgact atggatgttt
     6241 tgtgattttt gatgtaggaa ctaatataag tgtgttaaag agattagaga aagatttaca
     6301 cgattgtaaa atttcaaagg ttaatagcaa tgagttttat acttatataa ggcgacattt
     6361 aaataaatat agaagtttaa tactaaaatc tgttatattt gatactataa aagcgttgaa
     6421 tgttgatgct aaaatggata acaatgatgt tgataaggat attataaaaa aagatataaa
     6481 tgaaaatata agacaaagag ctgtaaaagg agaagtgtac catattgata ttataaaaaa
     6541 agatatgaaa gtaaaatatt ttgatagaaa ctatcttata aatttagata tatttatgag
     6601 agaagaagat aaaaattaag agtgggagtg atatatttgg caaaacaaaa gttttatgct
     6661 gtgaaaaaag gtaaaaatat aggtgtatat aatacatggg atgagtgcaa aaaacaagta
     6721 aatggattct caggagctga atataagagt ttttctactt ttcaagaagc taaagaatat
     6781 attgatgggt cagaaaaatt gagttttcaa gaagataagg agtttataga agcatatgta
     6841 gatggaagtt atgaacattc agtaaaaatg tatggttcgg gtgttgtgat tttaaaaaat
     6901 aatgaagtta taaaaactta tagtgaaaaa ggaaaagaga aaactttagt aagtatgaga
     6961 aatgtagctg gagagataga ggcttctaaa attgcaatgc agtactgtat tgacaataat
     7021 gttcaaaact tgattttata ctttgactat gaagggattg aaaagtggtg tactggagtt
     7081 tggaaaacaa ataaagaagg aactatagca tataaaaatt tctatgacag tattaagaat
     7141 aagcttaatg taaaatttac aaaggttaaa gctcattctg gaaataagta taatgaggaa
     7201 gcagataaat tagctaaaaa agctattggt gtataataaa aaaatattta tacataatta
     7261 catggtataa gagtatatct aaattcctac tttataatta aacaacttgt aattaaacaa
     7321 cttgtaatta aacaacttat aattaaataa attttgatta aaaaagcaga ttgtattatg
     7381 aaaaaataag tatctaattt ataaacaatt aataatagta ttaattagat acaacttatt
     7441 taatataata aaatctgctt tttatataac tataaaattt tcctacattc tattagaatt
     7501 gcccataaat accttcaagt tcttctttta atacttcagg gtcatttata ccaatagaca
     7561 tggttctagc cagtatattt gcttggtcat aagttgatgt ccatgagaat acaccaccta
     7621 gacaatttct aagaacatat tgagttttta agaatattga taaagcatca tcatatgaca
     7681 ttgcaaaatc tccatcctta actaaatatg gaacttgagc agtattatca aatctatatt
     7741 catatccatt tttatttata taatcccttc taagctcatc ataagttctt gttatagtag
     7801 cacctaatct tccataaaat ggtataccaa gaaggatttt ttcagaaggc attccagcat
     7861 tctcaagatt tcttaccatt gcatcaacac tatatcctgg caaagataag tctgaatcaa
     7921 aaagatttgc ttgatgtttt ctaccatttg ggcctgtttc acctgctgta aaatcataac
     7981 tcataagatt aaaataatct attataggag ctattttatc tatttcagca cttgaattta
     8041 tatatcctct atctcctgtt ccagctacac taagccattt atcatctcct ataacatctc
     8101 ttatggcagt tagtaagagt gtaaagtttt ctctatcttg aggtcttgat gttattccag
     8161 atgcactact tccaggatat tcccagtcta tatctattcc atctaaagca tattcattta
     8221 tcatctgatt gacctgtctt gcaaaattat atctagatgt aggtgttaat gctgcatcag
     8281 agaatccttc agctccccaa cctccaatag ctacaattac ttttaagcta ggtttttcac
     8341 ctttaagagc aagtaattga tttaaaaatc taggataagg aataaacaaa tttccagact
     8401 ggtctatctc agcaaaagca aatataactg catctaatag ttgagcatct acattagttg
     8461 gatttccaag aacatattct gctacaattg gatttatatc tggacagttt gcattttcaa
     8521 ctattggatt gtatgggtct gttatttgtg gtctaactaa ttttttagaa tcttttaaag
     8581 ttttcatagc tattttttta gtattttttt cgctagtata atcgtagttt ttataattat
     8641 gtttatagaa atcttcataa ctactatctt ctttgttata gctagaattt attttgtcac
     8701 aagattcatc tttatcatag ttaaaattca tttcatcaca agacttatct ttaaacttat
     8761 cttgtttgga gttgtgaact ttgttagtat ttttagtatg ttttttctca tgattgcaat
     8821 catctttaat atcagtcttg tgttcttgtt tacagtcaag agctttgttc aggtagtcat
     8881 gatgttcatg ttcacagtta cattcatttt caatgttttt agtatgttct tttttccagc
     8941 tacaagtatt atcaattttc ttgctcactt cttcatcatt ataattatct ggtacaaaac
     9001 ataagtacca gtccatacat gaatatttct tattacaact gttaactcta ttttttaatt
     9061 ccttatagtt tttaggataa ggtaaaatca ctggcattcc aggaaaccag tttgcaggag
     9121 taactatatt atctctatca ctagtctgaa gtgcatctac tattctaagt atttctggaa
     9181 tatttcttcc tgtagttagt ggataataaa gaatcgttct tagaatttga ttattatcta
     9241 taataaatac agagcgaaca gttgatgtat cactcattgg ttttgatatc atgccgtata
     9301 acttggcaat tctcatatct ctatcttcta taataggaaa tggaatttct acacctgtaa
     9361 gtaaagaaat attatacatc caagctaaat gtgaactgtt actatcaaca cttagaccaa
     9421 ttagttctgt atttcttttt ttaaattcgt cataatattt agcaaaacat aaaaattctg
     9481 tagtacaaac tggtgtaaaa tcaccaggat gtgaaaataa aacaatccaa ttacccttat
     9541 agtcagagag tctaatagga ccatttgttg tattggcttt aaaatcagga gcctttgacc
     9601 ctaaacttgg caaatttggc atgtaaatca cccctcaata aattatcatt tattttattc
     9661 tattaattaa tacatattag tgttactatt ttattaaaag ttaagtaatc cttatgtcaa
     9721 aaaaagatat ttattaaaaa gatttaatat tttcctaata taaatgacaa tagtaattgc
     9781 tattttttta ctaatttggt atagtaatgg ataaggggta gtgtcaattt tagtgattat
     9841 acgaggtgag tggattgaaa aaaattctaa gaattttagt tatctccatg atgatatgtt
     9901 ttgcaatgac ttttgcatta gtgttaataa attcagttat gggagtacat ataggattgg
     9961 attattactt tttgatggga agtgtgctta cagtttgttt aacaattgga attgttttat
    10021 gcaatgctag aaaaatattt gatagcaaag ctatacacaa gacaagaacg aaaactgtca
    10081 gtaataataa aaaacaaaag agtcattcta gggatataaa aagaaagata tcataattta
    10141 tgtaaaggaa atatttatgt ataaaataat gattgatagg taaaataaaa acacgattta
    10201 acttgtgttt ttattttttt tcaagaatta tgatataata accaataaga aatttagttt
    10261 accataaaat ataaagatta ttaaatggag agggaactat aatgaatttt agagatttat
    10321 ttgagaaggc tgtagatttt attgatgaaa aaaggaaatc tatagttgcg atttcattat
    10381 caacattagg tgtaattgct ttgataatag tatttttctt aagtagtaac gagtttagtg
    10441 taggaaatga agctaatgag ttgttaaaga ttatagagaa aagacagtat tctatagctg
    10501 ttgattacta cacaagtata gaaaaaaagt tttctgattc aaaaatggaa aggtttaata
    10561 agtctgtttc taaaaaaata aataaattgc tattaaatag tggtgataaa tatttagatg
    10621 gagacataag tcaagaaagt tttattggat taataaatac cgtgaaagaa ttaaacaaaa
    10681 taagtataga tgtggatgac ttattagctc aagctgatag agttagagaa atgtataaag
    10741 aagaaaatac tacttatgat attgccatta actacataag tactgtttca atattaaatg
    10801 gtatgggtag taacctagat gtatacaaac agaatataga aacaatcaaa gagtctagag
    10861 atgtttatga ctcagcagta aaagaccaaa aagcatataa gtattatgaa gctattgaaa
    10921 agtataataa agtattaaaa gaagatgaaa agtattacag tatggctcaa aatgggaaag
    10981 aacaatgtat tgaagaaatg tatgattact atataagtag agctgaagag gcaaatactt
    11041 ctggagatta tgaaagagca ttacaatata ttgagtattt aaaagacgat tattcagatg
    11101 atgaaaaggt acagagttta gaaaaaaaat ataaaaagaa tttatcattg tatactttaa
    11161 catctgatga cattataaat gtaatagcta aaaaaagtgg caaagacaag gcaaatctat
    11221 ctataaattc acttccacaa atgataaaaa ataataaata ctattatgct gaagtatatg
    11281 agtatgataa attaataaat gaagttttgg taagtgcaaa aactaaagat ttatattcat
    11341 ataaagatgg taaaaaagat tacaaagtag attatggtga tggatacttt agaatattag
    11401 aagatggaag ttatcaattt ggtattacaa aagataaagc taagtttgtg ttaactaata
    11461 ctcttgatga aaaagaaaat aagtataaaa agattgagat attagatata gagaaagcag
    11521 atagatatgt aaaaagtaaa aagagtttag aagaattgtt tggaaaatat aaaaatattt
    11581 attattatgc tgtagtaaat aaaggcttat ttagaggcaa agaagtttat gctattaata
    11641 tttacaatga aaaaatatat gccatatcag aaaatggact aaatgaatat taattaataa
    11701 tattaaatta atatataatt aaaaagtttt tggggtttta aggcgcatta tgtagtaata
    11761 taataatagg gatatataaa taattttatt gtaaggagaa gtatgggatg agtaatgtga
    11821 acaaaaagct agttatagct attggagctg tagcattagt agtaataatt tttataggca
    11881 taatgtgcat gcaatttaaa ggggagaaaa tagcaaaaaa cacatatgtt aatggagtaa
    11941 atattggaaa attaactaaa tcacaagcaa aacaggaatt agctaaaaaa tacaaattag
    12001 agaatgttga ttttaattac aatgataaaa gctggaaagt aaagagcaaa gatttaaatt
    12061 tatctactga cctggataaa acagtagaaa acgcatataa tttaaataga aagagtgctt
    12121 tctttggcaa tctatcaaaa actatttcag caaattttgg caaaaaaagc aacttagtag
    12181 ttgcgataaa ttatgataaa aataaattaa aagcagaaat ggaaaagata gctaaagaaa
    12241 tagatgtaga tgtaaaagat gctacacttg atataagtgg tgaaaaagtc aaggttatac
    12301 cagattctga tggtcttaaa atggatattt ctaagagtat ggagaatttt gataatcaaa
    12361 ctaaaaaagg taactataaa aatgagttag tagtaaaagc tactccagca aaagtaaaaa
    12421 aagagcaact tgctaatata gatacaaatt taggtacgta ttcaacaaca tttaaaactt
    12481 ctcaaataaa tagaagtata aacataaaat tagcaacaga taatataagt aatgtattac
    12541 ttatgccagg agaaactttc tcatttaata aacatacagg gaaaagaagt aaggaaaatg
    12601 gatataaaag tgcacctgta ataatggaag gtgagatgga agaagattat ggtggaggag
    12661 tttgtcaagt ttcatctacg ttatacaatt ctgtgctata cgcaggttta gaaattgtta
    12721 atgtaaaaaa tcatactata ccatcaagtt atgttccaaa aggaagagat gcaactgttg
    12781 cagatagtgg aatagatttc ttatttaaaa ataatctaaa gcatccagtt tacataaaaa
    12841 attacgtttc aggaaatcaa attgtttgca atatatatgg aagtgcagaa gataaacaaa
    12901 atataacaat atcaacaaaa ttagatggcg tttcacaaac tacaatgaaa agagttaatg
    12961 accctacaat gcctaaaggt aaagaaaaag tagacaaaag tggaagaaat gcatattctg
    13021 tatcaacata tagaacattt aatgatgcaa atggtaaaaa aattaaaact gaaaaaatag
    13081 ctaattcata ttatcctaag aaggaaggta ttatattagt tgggactatg gaaccaaaac
    13141 cagaggaaaa accaaataca gacgaaaata agaataatca aaatacaaac aatcaaaacc
    13201 caaataatca acaaaaacca gaaacaccac caactgataa taagcctaat gagacacaac
    13261 cacaaccgca agcttaaagt tctcaagaat aggatttatg tagagtaata aaaaatataa
    13321 atctaaatag gagggattac tttgaattta aataatataa cgaattacgt tcaaagtatt
    13381 tgcgagaata tttctaatgt cttggatgta gatgttactt tagttacaaa agatttaact
    13441 aggatagcag gtacaggaat attcagaggt aaaataggag agacaatatc agagaagtca
    13501 gtgtactctc ttgttttaaa agatgggaaa tcctatgtta taaataaaga aatagaagag
    13561 aactgtaata aatgtgcaaa tagagaaaat tgtaaagagt tagctgatat atgtactccc
    13621 gttaaaatgg gagagcatat acttggaatc ttgggtatag ctgcgtttaa tgaagaacaa
    13681 aaagaaaaga tactttcaaa agataaggag ttaactgctt ttgtaaggag tatgtctgat
    13741 ttaatatctt ttaaactaga tgaactttat aaacaagaag ttaagactat agatgacctt
    13801 gagagggaag ctatagaaaa tgccataaag aaatatggaa gtaatacaga aggtatgaaa
    13861 aaagtagctg aggcattaaa tataggtata gctacactct atagaaaagt aaaaaaattc
    13921 aacattaaaa gttaatagta ttgaaaaaga ggtaaccttt ttatgaaaag aagaggatta
    13981 ccttttttat tatgagaatg gtggaaaaat atgtatagtt ttgacttgac atattaaata
    14041 tgttatctta aatatatatt ttagagtgga ggtgagtatt attttagtta aagatataaa
    14101 acaagacata gaaagcatgc ctagaaaaat tttagatata tctttgatag aagataataa
    14161 aagtgatttg acttttttta atatagaaga agaagacggt gatgaaacat taaaagcatt
    14221 aaaacagagt tatgtgacta caaattataa agaagaaact ctattaaact tgcaaggagc
    14281 tttaaattca aagtcagata caacgtctaa ggagttaaaa ttagaatcat ataaaagata
    14341 taacaatgca ttggatttag catataaaaa ttatataact agtgccatta atatggttaa
    14401 taaagctctg gagataaatc ctaaagacat tgatatacta aatcttagag gtcttttaaa
    14461 attattaaaa tgtgattttg ctaaatcatt tgagagtttt tatactgctt tatgttatga
    14521 aaataatcat atatctagaa aatatgtaaa tctaatttca tctgaagatt ttaaaacttt
    14581 tttaggaaga tacaatcatt ctataagatt tataaatgaa gaattaaatt atgagtcaat
    14641 acatatttta gagaacataa ttgaagaaga acctgaacta atagagccat atgtaatttt
    14701 atctttgtta tatgataaac taggtaatgt aaaaaagaga gaaggatatt tagacaaact
    14761 taaagagcta gataaagata atcctgtatt tgaaaaaaat gaagaagaaa aagaagatac
    14821 aagtaaaaag gaagaaataa aaaaagtaaa aagaaagaaa agtattttgc catatattat
    14881 agttggctgt gttttaatag gtatggggat atatttgatt caaagcaaaa agagaataga
    14941 aaacttgaat aaccaaataa gtagtaaaga ggaaaagtta agtgaaactg ataaaaagtt
    15001 aggagaaacg agtaaaaaat tagaaaaaac gaataaggaa ttaaatgagg ttaaaaataa
    15061 agaaccagaa agagagataa ctgtagctga tgaggaagat ttatattatc aagctctaaa
    15121 tttaaagaaa aacaaagaat atgaaaaagc catagataat tttaaaagtg tagtttcttc
    15181 tggtaaaact aaaaaatata tttctgagtc aatatatcaa ttagcaataa caaataaatt
    15241 attaggaaat aaggatgaag ctataaaata ttacaaaaaa tatataaata cttatactaa
    15301 aaatgaccag tattatgatg attcatatta cgagttgggt atgctttatt atgataatgg
    15361 cgatttaaaa aatgcacaac agacttttta tagtttgaga agtgaagttc cagacagtat
    15421 gtataacaat tcaaaaataa aagaaatttt aagtgaaaaa taggtttata tagttaagtt
    15481 ttttgaaaca accaagtatg tattttatta aattaaaata aaaataaatt taaaaagaga
    15541 ttgtgtcaaa ttataacttt ctattttaaa gaaagatatt tgcttgttga taaaactaca
    15601 aatatatgat ttgaagcaat ctctttaatt tttggaggtg gaaaatgaaa gttttcaatc
    15661 aattggtaat aattttatct atctgggtaa taggagagta tataagttcc tttatacaag
    15721 gaattatact tattcctggg agtatagttg gtatgctatt attatttcta ctactacaat
    15781 ttaaagtttt agacttgagt agtatagaaa atgtaagtgg atttttcctc gataatatgg
    15841 ctatattttt tattccagca ggtgtatcac ttataaagtc attagaccta ataagggaaa
    15901 atgtattagt gcttttatta gtcatatgtt taagtacact aatagtaatg tatactactg
    15961 gaattattgt ggagaaaatg ataaaaaaga aggaagaagg acaaaaaaat gtataatgta
    16021 ttacagacac cagtatttgg tataatagta acaatagttt ttttcaattt aggaagatac
    16081 attcaacgaa aaacaagtaa tcctatatgt aaccctttat taattgcaat cgtaggaata
    16141 atattgtttt taagcatatc taaaatccct tatgagaatt acaagatagg agctgatagt
    16201 ctaaatttct ttttaggtcc tgtcacagta gtacttgcag ttccacttta taaacaattt
    16261 gaattattta aaaagcatat gtttgagatt attgtaggaa taggttgtgg tatagtgatt
    16321 tcttttgtat ctgtattgat aattggcaaa atagctaatt cagatgtaag tataataaat
    16381 tcactaattc caaaatctat aacaacacca atgggaatat ctttagcaaa ttctttaaat
    16441 ggtgtagaat caataacagt tgtagccata atttttacag gtatttttgg tggtatggtt
    16501 gcttctacag tattcaaact ggggaatata aatcatccag ttgctaaagg aattgctctt
    16561 ggaacatctg cacatgcttt aggaactaca aaggcttttg aacttggaga aatagaagga
    16621 gctatgagtg gtgtttctat tggagtctct ggtacaatta cagtaatact aattccaatt
    16681 ataatgaatt ttatataaaa aataaaatta agatgagtac cttgaatact taaaacaaaa
    16741 taaaataaaa aaaatttaaa ataaaaactg aacgaaaaaa atgcaaataa tgaaataaaa
    16801 aaatcatgta ctgaaattat aaaaagaaaa atttcattta ggaattaatt ataaagagac
    16861 aattactaaa cttgaatttg ctaaaataaa ataaattact gctaaaatag atatttagaa
    16921 aaaaatacta taaatttact atattgcaag ttaaataata agcagtatca tattatgcta
    16981 gaaaaagttt catttaaggc ataaatgaga tgatttgaac gtttttaaag attagaatat
    17041 aaaagattgc actagacata tatgaaaaag ataatataat agatgatgta atagatgaca
    17101 gttcaagaaa ttataaaaaa aatttatttg aaatattatc tattacatca ggaataatat
    17161 atgtaagagt aagcttttaa aattctaaag atttagaaag attgaaggga gaaagaaatg
    17221 agtgaagtta agaataccct acaaaaacat ccagttttaa gatattcaac aatgctattt
    17281 gggatgttga tgacatctat aggaataaat gggttcttaa gaccagcaca tctgcttagt
    17341 ggaggtgcta caggtatagc aacatctata aactatttga caaatataaa tgtaggttta
    17401 ttaacatttt taatcaatat tccaatattt atcttagggt tcatatacct tgaaaaagag
    17461 ttttgtattt caagtttggt aaatatgatt gtattttcat tattattagg agcaactcag
    17521 gaaataagta atattatacc tatacatgat atattgcttc aaagtgtata tggtggtata
    17581 ttatctggtt taggagtcgg agttgtattt agaactaggt cttctcaagg tggtacagat
    17641 ataatagctg caatactaaa aattaagaag aatatagaaa tgaaagacac tgctttagca
    17701 ataaatggct tgatagtact tacaggaagt tttttatttg ggttggattt agcattatac
    17761 actcttatag gtctatttct aaatgcgtat tcaatgagtt ttattaaaga tgcaatgaat
    17821 taccaaaaat ctgttatggt aatgtcaaat gaagtcgatt taatagcaga agatataatg
    17881 aagagtttag ttagaggtgt aactttttta gatgcagaag gtgcatacac tcatcaaaaa
    17941 aagaagatta tatatacaat agtttcatca aatgagatac caaagataaa agatatagct
    18001 ttgaagtatg ataaaaaggc ctttatatct gtaaatgatg ttacagaagt aaagggaaga
    18061 gggtttaaag caaaagattt atagaaaatt ttacaagctg ggagtttata atgtaaattt
    18121 gtatattata tctctcagct tatttaaatt tagtatacga aaataaaggt tttattaatg
    18181 atttgtgtat cactttaaaa gatatcattt taaaattaaa attgaaaaaa ttacttaata
    18241 tgtagaaaac atgaccacta ttaggatata ataaatttaa tagatttagt aaaataccta
    18301 ggaataacgt tggaggaatg gcattatgaa tttatctaaa gaatatgtta atttgtatga
    18361 taattatttg aaatatataa atgaagtaaa caaagagatt agaagtataa agaacatgaa
    18421 gaaacgtgtt ttaaaaaggg gcgattttgc tccggaaaat gaagtattag aattggaagg
    18481 aaaaacaata cgcgatatag atgatgtcga tacaaaaaaa ccaaaaaata aaatctataa
    18541 attttcaaat ggggatgtat atgtagggaa attcttagag ggaaagatgg aggggaaagg
    18601 ctcatatact ttttttgtag atgaaggaac agttatggag tacataggag aatttaaaga
    18661 agataaaaaa aatggaaaag gaaactttat attttcaaat ggaaatgaat atataggtta
    18721 ttttgaggaa gatatgatga gcggtattgg aaatatgtta tatagtagta aagatgaata
    18781 tattggaact tggaaaaacg gaaaaaaaga tggaaaaggt atttataagt ggagtgatgg
    18841 atgtatatat gcaggtgaat ttaaaggtgg aaagatggaa gggtacggta tttgctataa
    18901 tagtaaaggg gaagtacttt atgaaggaga atggaagaat aatttgattc atggtaaggg
    18961 aacttatata tgggaaaaag gtaaaaagta tataggtgaa tttatgcatg gtaaaaaaca
    19021 tgggcaagga actttttatc taaataatga attggtttat gaagggactt ggaaatttga
    19081 taagccatct atatttgata gaacattaga tgaaatattt tccttgagac tgtagcttta
    19141 tctaattagt agataaagct tttttctaat acttactgta aataggtatg aatgatattg
    19201 attagtatac actatcaata ttaacttaat aaatgcaata ttaaaaaatt ttgattgatt
    19261 ttaagaagga gagatatatt gaagagatta attgctttag tgttagtagt acttattgtt
    19321 ttattttata gtcctgaatt attaaaagaa ggatatgatt taaaagaaaa gttttttcaa
    19381 agtgaaatat ggaatgaatt tttagacaaa ataaatataa atgataattc taagaaagat
    19441 aaaaaagaaa aaagtcaaaa tttacaatca aataatactg aaggggagaa cttaggagag
    19501 tcagatataa agaattttga aaaaataagc ttaggagata atttaagcta tgttttatca
    19561 agtatcggca aacctggtag aattgatatt agtgaatatg gttttgattg gtatgtatat
    19621 aatcaatatg gaaaagaatt tgcaatggtt ggacttgaaa atgatgaagt agtagcactt
    19681 tatagtaatt ctattaattc atgtgaaaat caagatataa aactaaatca agatagacaa
    19741 acagtgagaa ctaagattac accattaaaa tataagagaa aaggaaatac cagatatata
    19801 ataaactcag aaaatcaata tgatataata agtaaggaag gtaagtatat tacaattttc
    19861 tacgatattc atgaagaaaa tagagtttgt tcatatttaa taattgataa aagcactgaa
    19921 gatgagttta aaaatttata tccagatgat agcgaagaac ttaaaaaatg ttttgaactt
    19981 gaagttatag accttgtaaa tagtgttaga aatcaaagag ggttaaactc attgagatac
    20041 agtgaacagg caactttgag ttcaagaaaa catagtgaag atatgagaga taataatttt
    20101 tttgaccatg taaataagaa gaatgaaact ccatttgaca gaatgaaaag agaaggtata
    20161 gtatatactt cagctggaga aaacatagca gcaggacaga ttaatgcaat atatgcgcat
    20221 gaagcttgga tgaactctga aggacataga aaaaatattt tggggaacta taataatatt
    20281 ggagttggag taatatttgg aggaagctat aaaacatatt acactcaaaa tttttataaa
    20341 taactaaaat atttgaaagg aagttaaaaa atgacatcat ggaaaaaaaa gacagtatat
    20401 aagtgcttaa tagcagtagc cttgttttgt ggaatagtgt taatatctaa ttttagtaaa
    20461 gtttctgctt tgatgatgga tactaatgga aatgttttga ttaaacatgg ttcaagggaa
    20521 aaaaagctaa tagcaattac ctttgatgat ggtcctcatc ctaaagaaac tagtcaagtt
    20581 ttagatgtat taaagaaata caatgtaaaa gctacttttt ttatagctgg aaaacatgca
    20641 aaatggtata aagaaccatt agtaagagca agtaaagaag gtcacgagat aggaaatcat
    20701 actttcaatc atcctgatat aagtaattta agttctagtc agatagaaga ggagattgtt
    20761 aagtgtgaag acattttgaa ggaagtaact gggaaaaaac ctacattatt tagaccacca
    20821 ttcggaagtt atagagaaaa agacttaata gaaattgcaa aaaagcatga ctataaagtt
    20881 gttttatgga caggtgtaga tgtgaaagat tggaaaaatc caggagctaa tagcattgca
    20941 gataaaataa ttaataaagt acaaaatgga gatataatac ttttgcatga ttatgcaact
    21001 aatgatacag ttgaagcttt agatatgttt attccaaaaa tgattgaaaa aggttttaaa
    21061 tttgtaacag tttctgagtt gataaaataa tttttagggg ggagatttac agtatgattc
    21121 ttatgataga caattatgat tcttttgtat acaacctagt ccaatatata gaagaattag
    21181 gggaagcagt tgtagtaaaa agaaataatg aaatcaaaat aagtgacatt gaagaactaa
    21241 atccagaggt aattgtgtta tctccaggac cgtgttctcc aaaagaagct ggtatttgca
    21301 tagatattgt tgagcatttt aaggggaaaa aacctatact aggaatttgt ttaggtcacc
    21361 aaactattgg acatgttttt ggaggagata ttataaaagc acaacagcct gtgcatggaa
    21421 aagtatatag tatcaaccat actaataaag gagtttttag gggacttaaa aatcctttaa
    21481 atgtcactag gtatcattca ctaatagttg actccaatac agttccaaaa gaattagaaa
    21541 ttacagcaat tactgataaa ggggaaataa tgggaattag gcataagaaa tatttaatag
    21601 aaggagtaca gtttcatcca gaagctattt tgtcagagta tggtcatgaa atgcttaaaa
    21661 attttataac agaagctaga gaaagggtac atatgtaata tgataagaga gataaatact
    21721 aaattaaatt cttttgagat atttactatt tttagaaatg aacatgacag ttttatatta
    21781 gatagtgcta tggataaaga aaaattggga agatattctt ttataagtag ccaaccattt
    21841 aaggtattaa agtataaaga tactgatgaa aatcctttag aagttttaaa agaagaacta
    21901 cataagtata gggttgtaaa tgatactaat cttccatttg taggaggtgc tgtgggatat
    21961 ttatcttatg atttaggtaa ttacatagaa aatttaccta gaactgctgt agatgatatt
    22021 gagatgcctg atatgtattt tggattttat aatcatgtaa tagttataga ccatcttgtg
    22081 caaaaaactt atatagctac tccaaacata gatatcgaat tagaagaaaa gataatagat
    22141 gatatagaac aaagaatatt aaaagaagag aaaaaaggta tagatagcat atgttatgaa
    22201 gaaaaagaag taacacctat aagacttaaa tctaatttta caaaggaaga atttaaaaat
    22261 gcagttcaaa gtgttagaga gtacataaga caaggagata tatatcaagc taatttaaca
    22321 caaaggttca gtggagaaac agaacttaca agctttgaat tgtataggga cttaagaaga
    22381 tttagcccag caccatttgg ggcattctta aactttgaag atgcacatat attatcaaat
    22441 tctccagaaa gatttataag atgtgttaat aagagaatag aaactagacc aataaaaggt
    22501 actcgtccaa gagggaaaga taaagaagaa gatttaagac ttcaacaaga acttagaaat
    22561 agtgaaaaag atagagctga actacttatg atagtagatt tagaaagaaa tgatattggg
    22621 agaatatcta aaactggaag tgttaaagtt ccagagctat ttgtaattga accatatgca
    22681 aatgtaaatc acttagtttc cacagttgta ggtgagttaa aagatgataa agatgctact
    22741 gatgtaataa aagcaacttt tccagggggg tctataacag gagcacctaa gattagagca
    22801 atggaaatta tagacgaatt agagccaacg caaagaaatg tatatactgg ttcaataggt
    22861 tatataggtt ttaatggtga tatggatttt aatatagcaa ttagaacaat aatcaaaaat
    22921 gacaaaaaag tctactttca agttggagga ggaatgactt gggattctga cccagatgaa
    22981 gaatatcaag aaacacttga taaagctaaa tccataatga aggctttgag gggatattat
    23041 gaagaataat gtaggttttg atagtagttt aagtaaattt ggaattggtt tgtttgaaac
    23101 tattagagtt aaaaatggaa tagcagtaga tttagatatc catatagaaa gaatgctaag
    23161 ctcaataaat tgtttggatt tagatataaa ctataaaaaa gatttcttga taaatgaaat
    23221 tgttacatat ataaaaaaag aaaatgtcat taataaagca cttagaatta ctgtttttga
    23281 tgaaggttat aatatatcta ttagggatat tccatataat caagagacgt atgagaaggg
    23341 gtttaaatta gctatatctc caatagttag ggggaatagt ttaattcata gacataagac
    23401 tactaattat tttgaaaaca tatataccaa aaatatcgca aataagactg gttataatga
    23461 tggtatattt ttaaattcag aaggtgtaat attagaatgt tctatgagca atattttctt
    23521 tataaaagga gaaagagtgt acactcctag tgatagatta ccgatattaa atggaataat
    23581 aaaaaaaagg attatagaga tttgtgacga attacatata gagttgatag aaaatgaaat
    23641 taatatatca gaaattagta gttttgattt tgtttttgtc acaaatagtc ttatgggcgc
    23701 tataaaggtt acagaaattg acaaaataaa atttaataaa gaaaatgttg tttttgataa
    23761 aatagtgaag ttattagaat aaattaaaat attaacaatt tgttggagac aaaagatgat
    23821 aggttgtatt tatgaagtat acaatgaaga gatgactaaa ataatagaaa accaaaacac
    23881 tttggctgta tatttagtgg gttctagtaa agatgtagat tttactcttt atgatgtagt
    23941 gataaacgat attgatgtgt tcgtttttgt aaaagaaggg gaaaaacaag agagaatact
    24001 gtttaaaaaa aagggaatag aatttgatgt aaattacttt tcaaaagatg gtgtaaaaaa
    24061 attattatca aatagggaat atttctttgt gaaagaaatg aaagatgcta aagtactgtt
    24121 tgatagggag aatatatcac atattataaa agatataagt agagatattt acttagaagg
    24181 acctagtaaa atgtcattag aggaaaagtc ttttataaag caagatattg gagcaaaaat
    24241 attgaattta aaaaataaag aaaaatttga tgtttttgaa tatcattttt tgacgaattt
    24301 atatttaaaa gatataataa ttgggtactt taatataaat gataaatggg taccaaaaga
    24361 caaaaaattg ctaaaagttt taaaaaaaga aaataatgag ctttttgaac tagtgtcaaa
    24421 agtaagtgaa aattatgact atcaaagact tttagatgtt tataattata tttttaaaga
    24481 aattgagaca aaagaggtta taaaactaat cttctagtca caaatcaatg gggggagttt
    24541 aaatgaataa agttgataaa gaaaagatac aacatgcagt aagagaaata ctagaggcaa
    24601 ttggggaaga cccagataga gaaggtctaa tcgaaactcc aaatagagtt gctaggatgt
    24661 atgaagagat attctctggt cttagtgaag aaccaagaga ccatttaaaa gttctttttg
    24721 cagatgaaaa acatgaagag ttagtattgg taaaagatat tccattttat tcatgttgtg
    24781 agcatcatct agttcctttc tttggaaaag ctcatattgc ttatttacca aaaggaggaa
    24841 gacttacagg gctttctaaa cttgctagag taatagatac attggcaaaa agaccacaat
    24901 tacaagaaag aatcactaaa aatgctgcag atataataat ggaagaatta caaccttatg
    24961 gtgtcttagt agtagtagaa gctgaacata tgtgcatgac aatgagaggc gtaaaaaaac
    25021 caggttcaaa aactgttaca tcagcagtga gaggaatatt tgaaaaggac attgcgtcta
    25081 gagccgaggc tatgagtttg attactatga agtagtaatt tattatagat ataatcattt
    25141 attgagataa aatttaattt aagggagtgt cattaattgt gtttaattat ggtaaaagaa
    25201 cttatataat gggaatatta aatgtaactc cagatagttt ttctgatgga ggagatttta
    25261 ataacttgga catagcaata caacatgcaa aagatatggt tgaccaaggt gcagatataa
    25321 tagacctagg aggagagtct acacgcccag gtcatagcta tgtagactca gacgaagagc
    25381 taagaagagt aatccctgta ataaaaaaac ttaaacagga attggatata ccaatatcaa
    25441 tagacactta taaggcagat gttgcagaag aagcattaaa attaggtgtc actatggtaa
    25501 atgatgtttg gggtcttaga aaagataaaa atatggcttc tgtaatagga aaatatgatg
    25561 cagaagtgtg tataatgcat aatcaagatg ggacaaacta tgacaaagat ataatggagt
    25621 caataaaaga cttttttaag gtaagtatag aaatggcaat gagttgtggt gtaaaaaaag
    25681 aaaaaatagt acttgaccca ggtgtaggat ttggcaagga ttttgaacaa aatattgaag
    25741 tattgagaag attaaatgaa ttaaaagatt tagggtatcc tattttgctt gggacatcca
    25801 gaaaatcagt aataggaaaa gtacttccag ttgagcctaa aaaaagatta gaaggtacaa
    25861 tagctactac tgttcttggt ataagagatg gcgtagatat agttagagta catgatgttt
    25921 atgaaaattt aatggcagct agaatgactg atgcaatata tagaaagtag gtataatata
    25981 atggataaaa tattacttag taatctagga ttctatggat atcatggtgt tcttaaagaa
    26041 gaaaattttc taggtcagaa attctttgtc gacatggaac tttatattga ttcaagagaa
    26101 gcaggtctta gtgacgatat aaataaatct gtaagctatg ccgaggttta caatgttgtt
    26161 aaagatatta ctgaaaataa acaatttaat cttttggagg ctttagcgga aaatattgct
    26221 gaagaagtct tgaataaatt tatacttata aatggagtta tggtcagagt tagaaagcct
    26281 gaggctcctg taaatggtat ttatgactat tttggtgttg agataaggag aacaagagat
    26341 gaataaggct tatttgggaa taggtactaa catgggtgat agatttgata atttatcaag
    26401 agcatgtgaa cttttgaaaa atagtgattc tatatataaa gtaaaagaat caagcttata
    26461 cgaaacaaaa ccatggggat atacagaaca agcagatttt ctaaatatgt gtgtagaaat
    26521 agaaacagag tttgaacctt atgagttgtt agaatattgt caagaaatag agagagaact
    26581 acacagagag agaatagttc attggggacc aagaactata gatgtagacg tactattttt
    26641 taatgatgta gtttccactg atgaaagact aacaatacct catccaagga ttcaagatag
    26701 agctttcgtt ttgataccac ttatggattt aaatgaagaa cttataataa atgaaaaaac
    26761 aataaaagaa catttaaacc tcctatctgc tgaagaaaga gaagaagtaa aggagcttgt
    26821 aggttatgaa agaaaaccta tttaataata attcaaagat agagataaat acaaataaca
    26881 aaaagataac tgtaaaaaaa gataaactta taatagctgg accatgtgca atagagagtt
    26941 atgagcaact tttggagact gctaaatttg taaaaagtca gggagcaaat attttaagag
    27001 gtggagcata caagccaaga acatctccaa attcatttca aggattaaaa aaagaaggtc
    27061 ttgaaatatt aaaagcggtt aaagacgaag ttggtatggc tgttattaca gaacttatgg
    27121 atgtaagaga tatggacgaa ctgtatagta taagtgatat aattcaaatt gggtcaagaa
    27181 atatgcaaaa ctttactctt ttaagtgaag tgggaaaaca aaataaacct gttatgttaa
    27241 aaagaggaat agcttccaca attactgaat ggataggtgc ttctgaatat atagcaatag
    27301 aaggaaatag taatattata atgtgtgaga gaggtataag aacgtataat gattatacta
    27361 gaaatacatt agatttagct gcagtaccca ttattcaaaa ggaaacaggt cttcctgttg
    27421 tggtagaccc aagccatgcc actggagtta gatatttggt aaaacctatg tcactggcat
    27481 cttttgcatg tggagcagat ggaataatgg tagaagtaca tcctgaccca gaaaatgctt
    27541 tgtcagatgg tgctcaatct ctttgcttta atgaatttga agatttaatg aaatcaatta
    27601 ataattatta aaagaggcaa gagcctcttt tttattcgta atcgttgcta aggtaaattt
    27661 ataagttata atttgatagt tagattattt aatataaata aagatttaat attgatagaa
    27721 ttaatataaa aaataaaaat tttttgatta ataagaagga tatcaataaa aaaagtcgaa
    27781 ttgtgtatat tgagtgattg acaaatttag aatatagtgt agaatagaaa tgttaacaca
    27841 aaaaattaca ccagaaggtg ataatatgaa tgatataaaa gacattattg aagaaataaa
    27901 agctagatgc gatatagcaa gtataatatc tgaatatatg aatataaaac aatctggagc
    27961 taattacaaa ggattatgtc catttcatgg cgaaaagaca ccttcttttt atataaatac
    28021 gtcaaaacag atttataagt gttttggttg tggtgaaggt ggagacgtaa taaattttgt
    28081 catgaaaatg gaaaatttgg attttatgga tgctgtaaaa attttagcaa ataaatgcgg
    28141 tatagaaatt aacactaaca tgaatgaaga aactaggatt aaaatagaaa aatctaagaa
    28201 atttcaagat attcatacgg aagcagctag attttatctt tcaaacttat tgggaagcaa
    28261 aaatcttggg tatgaatatt taagaataag aggactagat gataaaatta ttaagaaatt
    28321 tggtttagga ttttctttgg attcatggaa ttcacttatg aatactctaa tcagtaaagg
    28381 atataaaaaa caagacttat tggagtgcgg tctaattgca aaaaatagag atggaactaa
    28441 ttgctatgat aaatttagaa atagagttat gtttcctata ttcgattata gaggaaatat
    28501 aataggattt gggggaagag tattagacga ttctttgcca aaatacttaa attctccaga
    28561 cacgttaatt tttaataaaa aacagaactt atacggatta aattttgcta gaaaaaattt
    28621 agaaagtaaa actatagtct tagttgaagg atatatggat ttaatttcgc tttatcagta
    28681 tggtataaaa aatgtagtag caacccttgg aacagccttg actgaacaac aaggcatttt
    28741 aataaagaga tatgcggaca ctgctattat atcttatgat tctgatgaag caggaataaa
    28801 agcgacttta agagctatag atatacttac taaattaggt atcaatgtaa aagtgttaga
    28861 cttaaaagat gctaaagacc cagatgaatt tgtaagaaaa tacggactta gtgattataa
    28921 aaaagctatg gatgtttcta ctcactacat aaaatataaa atagatcatc ttaaaaaaga
    28981 atttaacatt caaaaagatg aagaacgagt gaagttcgca aaagaagctt ctaaaattat
    29041 aaaacaattg acaagtcctg ttgaaattga tttttataca aagtatttaa gcaatcaaat
    29101 agatattaat gtcgagtcta taaagagaga agtttatggt aaaaattaca ataagccata
    29161 taataacaaa aatcaaaaga aaatagaaga aaaagtaatt gaaaaagtcg aagtcagaca
    29221 agacggaaaa caattagttg aagaaactct aataaaaata atgttagaag acaaaaaaat
    29281 tagagaaata gcgttactta aagtagaaga aagtgatttt ttattgaaag aaagtaaaga
    29341 aattttaaat tacatgatta aaaatcaaga attggacaaa ataactattg acaaattgaa
    29401 aagtttaaac atatccgaag aatacttaaa agaattaaat tcaatttctt tgaatagtat
    29461 aaacttagag aacacaaaag aaatagaagg aataataaca aatatcagaa aaaactcatt
    29521 agaagagcaa attaatagct tgttgagaga acaacaagaa ttagaaaata ataatgatat
    29581 gaaagaggta gatggtagag ttatggaaat tgctttaaaa atagttgaga taaataaaat
    29641 tttaaagagc ttgtagagaa aggaggtttg agtgtggaaa ataaatcgaa taaaaaagag
    29701 ttaaaaaagg ttactgctaa gacattaata gaaaaaggaa aaaagcaagg ttctctgaca
    29761 cttgcagaga taatggaagc tttttcggag actgaacttg ataaggatca agtagaaaat
    29821 ctttatgaga ctttaggaaa tttgggaata gaaataacag aaacaaaaaa ctataaagct
    29881 gatatagatt tttcggttgc tgatgacgat ttaagtatag gccacttaga tgaagatgca
    29941 gaggcaattt cacatgatga ctcttctgca atagaaatag aaactgtgga tttatcttta
    30001 ccaaaaggga taagtataga tgaccctgta agaatgtact taaaagaaat aggaaaaatt
    30061 cctctactta aaccacatga agaagtagaa tttgctagaa ggatgcacga aggtgatgag
    30121 atagcaaaac aaaggttagt tgaagcaaac ttaagactag ttgtaagtat agcaaaaaga
    30181 tatgtaggaa gaggtatgct tttcttggat ttaatacaag agggaaattt aggtcttata
    30241 aaagcagtcg aaaaatttga ctatacaaaa ggatataagt ttagtactta tgcaacatgg
    30301 tggataagac aagctataac acgtgctatt gcagaccaag ctagaactat aagaatacca
    30361 gttcatatgg tagagactat aaataagcta ataagagtat caagacaact acttcaagaa
    30421 cttggaagag atccaaaacc agaagaaatt gcaaaagaaa tggaaatgac agaagataaa
    30481 gtaagagaaa taatgaaaat agctcaagac cctgtgtctt tagaaacacc aataggagaa
    30541 gaagaagata gccatttagg tgactttatt ccagatgatg atgctccagc cccagcagag
    30601 gcagcagcat attctttatt aaaagaacaa atagaagatg tacttggttc attaaatgat
    30661 agagaacaaa aagtattaaa gcttagattt ggtcttgagg atggtagagc cagaactctt
    30721 gaggaagttg gaaaagagtt tgatgtaact agagagagaa taagacagat agaagcaaaa
    30781 gcactaagaa aactaagaca tccaagtaga tcaaaaaaac ttagagatta tttagactaa
    30841 aaagatacat cattgatgtt tttgtgataa atgccatgag ataagtgata tctcatggct
    30901 attttgtagt aaagatttaa ttttattttt gtagttattt cagtctaata tctataactt
    30961 ggattaaatc ctataaataa agggaattga aaggagaatt atatttgaaa ttaacagata
    31021 gattattgaa aatagcatca ttagtgtcag atggtaaaaa aatagctgat atagggacgg
    31081 accacggata cataccagta tatttgttaa aagagggaaa agtaccattt gcagtgctag
    31141 cggatgttaa taaaggacct cttgacaatg cacataaaga agtcatacag aacaatcttt
    31201 tagataaagt tgatttacga ttgggttctg gtatagagat acttgaaata ggggaagtag
    31261 aagaagtgat aattgctggt atgggtggaa tcttgataag tgagcttctc gaggctaaaa
    31321 aagaagtagc acataatgta gaaaaattaa tattacagcc aatgcaagca caagaagaac
    31381 ttagatatta ccttttaaac aacggatatg aaattttaga agaagtttta gtgagagaag
    31441 attttagaat atatgagata attgttgcca aatatacagg taaaaatact ataatagaag
    31501 atgagatata ttatgaggtt ggaataaagc ttttagaaaa caaagattct ctttttaatg
    31561 attttataga aaaaaagata aagacatata gttctatagt taataaatta gaagggaaaa
    31621 atggggaagc tatagataag aagagaaagg aaagcgaagt tgcaataaaa aaacttgaaa
    31681 atttaataaa ataaatagga ggtatatatg ctattaaaga gtctaactag aaaaattgaa
    31741 aaaaagtatc ctctaaactt agcagaggat tgggataatg taggtcttat agttggagat
    31801 tttgatatgg atgtaaaaaa ggtattggta tctttagaag ctaatgaaga tgttattgat
    31861 gaagctatat ccaaaaatat agatttaatt gtgacacatc atccttttat atttgggaaa
    31921 attaataaaa ttaatagtgg agatttaaaa ggcagactta tccaaaaact tattaaaaat
    31981 gatatatcac tttattctat gcatacgaat tttgatatag catttgatgg tctaaatgac
    32041 tattttatgg aaattatgga atttggtaat tcaaaggttt tagatataac taaaagcgaa
    32101 aatttgtata aattagcagt atacgttcca cataactatt cagatgaact tagaaaggtt
    32161 ttaagtaatt caggagcagg tcatataggg aattatagtg attgtacgtt ttcaatagaa
    32221 ggtgaaggac aatttaagcc attagaaggc tctaatccgt ttcttggaag tgtaaatgat
    32281 atagaaactg taaatgaagt taaaattgaa actgtagtac cacagaagct tttaggtggg
    32341 gtaataagtt ctatgttaga tgcacatcct tatgaagaag ttgcatatga tttgtacaag
    32401 ttagaaaata aaggtgagat atttggacta ggtagaataa gtaaacttga taaaagtatg
    32461 acattagaaa gtctttcaag gaaaataaaa gagaaattaa atatgaaaca cataagagta
    32521 gttggaaacc ttagtacaga cataacgaaa gtagccgttg taacaggagc tggttcagaa
    32581 tttgtaaaaa aagctaagag acaaggtgca gaagtactaa ttacgggtga tgtaaagtat
    32641 catgaggcac aagatgcact tgatattgga atgtgtatag tggattgtgg tcattttgat
    32701 acagaagata tattcaaaaa tgttatgaag agatttttag atgaatttag tgaaattgaa
    32761 gtaataaaaa gtaatgtata tttgaatcca tttagtacta tttagcagtg tttgataata
    32821 aaaatactat ataaaaaata aatttgagtt taattagggg gataatgatg aaagtagcag
    32881 taatttttca tagtgtttgt gggagtacat atttattagc tagagaatat aaagaagcgc
    32941 ttgaagaaat gaatatcgag gtagatatat ttagagtaag tgacgaagtt gcaaaaacac
    33001 ttcctcaata ttatttgata aattccaaag aatacaaaga cgaatttgaa agtataaatg
    33061 taattaaatc tggaaaagaa atattagatt atgatgctat atttatgggt tcaccaacat
    33121 attatggaaa tgtatctgga ccaatgaaga tgtttatgga tagtttttct gatatttggg
    33181 taggtactcc tttgagtggt aaaatatttg gttgttttgc tacagcaggt tcacaacatg
    33241 gaggaggaga attagctttg caagctatga atatatttgc ccaacatatg ggaatgactc
    33301 ttttgtcagt tccatgttca gtaagaggag gttatccagc gtatggaata cttcatatag
    33361 caggagataa ttctgatata agaccaaatg atgatgccaa gataggtata agagattacc
    33421 taaaaagact taacatataa gaatatcata taaaaatacc taaaagcgac tgtatatact
    33481 tttaggtatt tttacttaat tataaacgtg aagctttacc agaaatatgg tcaatttcta
    33541 ttttaacaac agttgttaag ttcatatcct tctctatgta cttcatgcct tcttcaaaga
    33601 atcctttaga atattttttt attatttcta caagagcatt tttcttttct tcattttcta
    33661 cagtgcaagc ttttccaaaa actatagcac tagaataagt agtactaaat ttatctggaa
    33721 taacactttc attagcaact actaaaaaag aaactttgtt gtttttcgat atattatcta
    33781 acttatgccc atttcttgca caatgaaagt aaatagaatc attaaagtat acataattta
    33841 cagcaactcc ataaggataa ccattttcag atatagtgga aaaagtacca aattcaccat
    33901 tttttaatac ttcaacagta tcttcttttg tcatttctct ttttttcaat ctcatttctt
    33961 taaacataat atcatccttt ctaattttga tgcttaaatt aaattttaga acatttataa
    34021 agattatgct atgtttttta caaattttaa gaaaaaaata tagtttaaga taaaaggtag
    34081 tgaaagagaa gctatttgga ggtttattta tgatattatt gttagagtag tataaacaat
    34141 actaataaat ttaatagctg gtttcttgtt atttaagata gtaagcatgt ctataagaag
    34201 gggaagttat cgagggtaga agttgttatt tataactata ttataaagaa aatattatta
    34261 tataaaataa taaatagtaa agaggtatca agttgaatat aatacttaat acctcttcaa
    34321 aattaagtta taaaagtgtt gtgggaattg tcctatacat ggtaaggaga taataaagaa
    34381 tgtatcttta aagtctcaac taaacagcaa aatttatcca acaaacatac aataaatgct
    34441 tctttgtatc taggtggctt aacagtaagt ggccacatgt gttttaaaat tatgtccgtt
    34501 tctttttcat ttatttgaaa aaataaagtt gcatttttta atgcttctct agggtgagta
    34561 aatccatgta atccttttct atcacctttg taatgccaat cataaagaaa aaagtcgtgt
    34621 agtaaagctc ctctaataac actttgaata tcaacactta agtgcaattt tttacataat
    34681 agataactat aatatgctac tgacaaacta tgttcaagac atgaaacatt gccatgatga
    34741 ggaaatgttc tcataagttg aactttttca gaagaaagta tctcagaagc acattcatta
    34801 aaatatttga tttcagcttt tgacaacatt atgaaaatct ccattcttag taatatataa
    34861 atactatact tcaacgataa tatgaaatat ttcttatcct acaaaatata atacccaata
    34921 atacaagtaa gatacctttt atggtatttt aaagtaagag agattttatt aggctaaaca
    34981 atgaaaaaat taaaagtaaa gctatagcaa tatatagtat ttttccaaga ggattgccaa
    35041 aatttatagt ccatccaata ccaaaccttt tattaaccat taatgatggg tcatttggat
    35101 tattatacat gatgcctgct atccaatact tctcatcttc ttctggagaa taagatgagt
    35161 tgaattttaa atttggggaa ttaatatatg ttgctattaa ataaattata actaaaagcg
    35221 ttgtaatatt catagcagtt gatagttttg tttttattga taaaagagta gtaaaaaact
    35281 gtgttgtcat tataatcatc aatattaaaa acaaataacc aattttattt aaatatttaa
    35341 tgttttcaac tctactttct tcaattctat ttgtatctat cttaatcctt gactttatgg
    35401 atccaatgga agttatatat aataaaacca ttaaacaaaa gtcagtaccg attaatttaa
    35461 atacattaag aaatgattta tctataaaaa catcaggaga cccatttatg ttccaatgag
    35521 tggcaattga gtctggcaat tgattataat taaatgctat aaaaatactc attcccaaag
    35581 taaatagtat cggtattaaa tggattattt taaatttttt tattatttta tttttcttat
    35641 ttataaaatc catatctact attaattttg aatctatatc tatatgacct aatttagaat
    35701 aaatttctga tttaagcttc ttagcttttt tatgtgtatg aatatatagt atactttcat
    35761 ataataaaaa acctataata ccaataaaat aggaaagttc caatttatta aatgtaaaga
    35821 ccagaataaa aactaatata aagtctataa taaaacctaa agataatagt tttttaaatt
    35881 ctttatctaa ctttataaag taattataat ttttatgctc ttggtctata gaaactccat
    35941 agatgtgttt tttacctaca aaatagggca taaaataaaa tgtagcatac atcaaaatta
    36001 gaactggaaa tattgtaaat agacattcaa tcttactcat aatatttcac ctcatacttg
    36061 tcaaataaac ttgaacttag tgacaaaaat tcttctttac taatacctga taaataagat
    36121 tttgccacca ataattccaa gtcaagttta tttttttgac gagttgtatt atctattttc
    36181 attggtagtt gagctacaat agcacctttt cttctatcaa tatttaagaa tccctcttct
    36241 tttaattcat tgtaggattt gttcactgta tgtaaattta caccaatatt ttcagccata
    36301 cttcttacag atggaagtga ttcatttatt ttcaattctc ctgaagctat agccttagtt
    36361 atttcttctt taatttgtac ataaatagat ttatcactat taaaatctaa atttaaaacc
    36421 ataaaaaacc tccattatgc tttataaatg aaaacgattg ttatatacat agtataacat
    36481 aaaaaataat aacaaacaca aaatataagt ttatattttg taaaaattgt aatttaaaaa
    36541 ataacataat ttttattaaa ataatacaga tactatttat agatagaaat aaaaattaga
    36601 aaggagaaaa aatatgttaa catatttaat gaaaagacaa atgcataaaa aagatgatgg
    36661 aatgagtaaa ccaatgatga ttacaggagc tgtaattaca ggtgtaggag catatgctgt
    36721 atataaaatg gtaaaaagcc ataaatctaa ttctcaaatg gtcaatacaa attattatgg
    36781 aaatagcgat tatgatgatt ataaatatga cgaatttgat tatgactgtg attgtagaga
    36841 cacaaaacaa gattatgaat atgaagaatt aaaaaaaaaa gtaaaagaat ttaattcaag
    36901 aagaataaat tgtgaaaatt gtccacatgt ttctcaagaa gaaatgatgc aatatgtaaa
    36961 tagtgttaaa gatgtaaagg aagagattga tttacatgaa gatgctaaaa aagataatat
    37021 ttacaaagaa gatgaataca aaaaatatga agaagaataa gtaatatttt gtataactga
    37081 acaaatagta atgtcaaatt atttaatatc tagttttaat atgtaagttg taacttaact
    37141 tgaaagaatc agtatcattc tttagatagt taagtaacaa cttacatttt atttttataa
    37201 attttaatag ttctttgtga ctattttgta attttagaaa tattttagta tccagggtaa
    37261 ttgtgtataa taaaacttgt atagatagat agtatgaaat taagcagata gtttaaaatt
    37321 aaaatgaaaa gaaaggaaat agttatgtta aagttgttga tagttgaaga tgatagtact
    37381 atatcttttg gaataaagta tgctttagaa caagaaggat ttagtataga tatatcaaag
    37441 gatttatcta gtggaaaaga aatgatttct tctaatgaat atagcatgat actgttggat
    37501 gttacattgc cagatggaac tggttatgag ctttgtcaat atataagagg attctctcag
    37561 gttcctataa tattcttgac tgcttgtgat gaagaagtaa atattgtaat gggtctcgat
    37621 ataggtggag atgattatat aacaaaacct tttagaatta gagaattaat atccagaata
    37681 aaagctgttt taagaagaaa aggtaatact tcagaagaaa ataagaaaat acttaaattt
    37741 ggagatttaa gtatatatac attagaagct agagtatata aaaatgataa agaaatattt
    37801 ctgacatcag ttgaatataa acttctttta attcttatac agaataaaaa tactgtttta
    37861 agtagaactc aaatattgga aaaactttgg gatgtaactt atgattttgt aaacgacaat
    37921 actctaactg tttatataaa aaggcttaga gaaaaaatag gtgatgattt atgtgaacca
    37981 atctacatag aaactgtaag ggggattggt tataaatgga taggaagtga aaatagtgtt
    38041 tctatataat ccagaagtga agaagttttt aagtaagtat gttactttga tgtttattgt
    38101 tatcataata tctattggat ttagcataat caacgtgtcc ttaactaaag agatggttgt
    38161 aagaaataat caggctataa taggaacttt atcaagcaag tatccaaact tggaaagtca
    38221 gatagtagac ataataactc aagggaagtc tatggagaac atagattatg gtcagaaaat
    38281 attaattaag tataattatg ataaatctat taggataaac tctgagccta ttatttctaa
    38341 attagtttta gagactatca aaataaatat aatattagta tgtataatat ttattttgat
    38401 atttgtgttg gttgttagat attttaagtc aatttacaat gatttatcag atatgactaa
    38461 gtatgtttac tatagttcag agggtaagcc atttgatatg aaaaacaaga atcaagaagg
    38521 gcagataggt cttctaaaaa ctgaactctt aaagatgaca acaattctta atgaaaaggt
    38581 tgaactttta aaaagagaaa agatattttt aaacaataca atttccgata tatcacatca
    38641 attaaaaact cctatgactt ctttaataat gttgaatgat ttattatata atgatgtacc
    38701 atacgaagta aaaatcgatt ttttaaataa gataaaaaat caattaaata gaatggattg
    38761 gttgattaaa agcatgctta aattatcaaa ggtagaagca aaagtgataa attttaaaaa
    38821 agataaggta aaatttagtg aactcataca tagagcaatg ctatctatga aaatacctat
    38881 ggagattaaa aatcaaaaac tgactataga gggaaatgat aatgtatctt atattggtga
    38941 tattgattgg tccgtggaag cattagtgaa tataatcaaa aattgtgtag aacatactcc
    39001 tgaatttgga aatataatta taacctataa agaaaatcca ttattttctg aactaataat
    39061 aaaagatgag ggtgaaggta tagataaaaa agatatacca catgtattta aacgattcta
    39121 tagaggaaga agtagttcaa aagaagatag tgtaggaata ggtcttgcaa tgtcaaaatc
    39181 tataatagaa agccaaaatg gcgatatata tgtaaacagc gaaaaaggtc agggtacaga
    39241 atttcatata atatttcata aaatgtatga tagtgactaa tctgtaatat ttaattcact
    39301 ttatagtaat agccaaaacc taatatatga agtatagaat ataaatattg gaggtagtta
    39361 tggaaatttt aagagtagaa aatctaacta aaagttatgg taaaaatgaa acaaaagttg
    39421 atgctataaa aaatgtaagt ttatcagttg aaaaaggaac gtttatagca ataacaggtc
    39481 caagtggaag tggtaaaagt accttattac atttattagg aggagttgac agaccaacta
    39541 gtggtaaggt ttatataaat gatgttgata tatataattt aaaaactaag gatttggcga
    39601 tatttagaag aagaaatata ggtcttatat atcaatttta caacttaatt ccagtcttaa
    39661 atgtgaagga aaacatattg cttccagcag agcttgacaa tagagatatt gatagagaat
    39721 attttgatga tttaatgaaa acattaggtc taattgatag agaaaagcac cttccaaacc
    39781 aattatcagg aggtcaacaa cagagaacct ctataggacg tgcattaatt aatagacctt
    39841 ctataatatt agcagatgag ccaacaggta atttagatag taaaaattca aaggaaatat
    39901 tagaattgtt aaaattgtca gtaaaaaaat acaaccagac actaataatg ataactcatg
    39961 acaaaaatat ggcacttcaa gctgatagaa ttataagcat agaagatgga atcataaaaa
    40021 gtgatgaggt gatgtagatg aatttatata cattactaac cattagatac ttaaaacaaa
    40081 ataaaagaag aaccattgta accataatcg gtataatact ttcaacagct ttaatttgtg
    40141 gtataggaaa tatatttgaa agttttatgg attatcaaat gagagaaact ataaagaatg
    40201 atggaagttt ccatgttaca ttttatgatg taaatagaaa aaatgtggaa tatgtaacga
    40261 aatcagctga aatagaaaaa catgcattta ccaaacaact tggatattct aaattagaaa
    40321 atagtgaaaa tgctatatta agtataaagc aatatgataa aaatgcaata aatggatata
    40381 aagtttctat aaaagaaggt agatttccag ctaaggaagg agaaattgtt ctaagtgaaa
    40441 gtattattaa tcttatagat gataagttaa aaataggaga taagataact ttaaaagtag
    40501 gggatatgtt tgactcaagc aagaaaaaga ttgatagtat gacatttcat gaaggtgatt
    40561 atatagcaaa tgaaaaagat agaactttta aagttgttgg tataattgaa aaacctgggt
    40621 ttgaacgtta taatggaatt gcaacagcta tagcctactt taatcctatg aataattaca
    40681 atgatacaat taatgtatca gtagcagtaa gaaatcctaa agatgtgtat aagataagta
    40741 ataaaataag taaaaatata tattcaaata aagataaaga agcagtttct gatatgataa
    40801 aatacaatga acatttattg aggttacaag gtgctagtaa gtattcaaat ataaataact
    40861 ctataaagtc tataattata gcagtaacta ttttagtaat catatgtaca attgctacag
    40921 tgtacaattc ctttagtatt tctataaatg aacgaaaaaa acagtttggg atattaaatt
    40981 ctataggtgc tacatctagt caaattaaaa gattagtttt tatagaaggt attataatta
    41041 gtttaattgg tataccaata ggactaatat ctggaacagt agctatagac ctattgttta
    41101 aaattataaa taagtatttt actgaatcgg ttgttactaa gatgagtttg cagatagtgt
    41161 ataatccaat aataactatt gttagtataa taatagtttt gcttacaatc tttatatcaa
    41221 ttctactacc tgcaatatcg gcttctaata tttctcacct taatgtgatt aaaaatactg
    41281 gagagtataa agtgggcaaa atcaaaagct caagacttat taaaatgatt tttaaaactg
    41341 aaggagtact tgcatataaa aatattagga gaaatagaaa aaaatttata ataactcttt
    41401 tttctttaat ggtaagtata ataatattta tatcttttag tggctttaca ttacttttac
    41461 taagaggtga agaaattaga aattctcaaa gaaaatatga tttgtattta actgcaaaga
    41521 acacagctag gtctgtagag gatactataa gtgaactaga agatattgat gggataaaaa
    41581 actttgaatt ggcaacagga caatatgtgt ctataagagt aagtgaaaat aaaataaata
    41641 aatccaagga agatttaatc aggaaatatt atcaaaaata taagattgga gatagttatg
    41701 aatatgattt cagtaataat gaattaattt ttccaggaga ttttgcagta aaaaacaaaa
    41761 taaataactt agtacagggc tcttttaata aagagagagc aattgaagaa aatggtgtta
    41821 tattagttag aaaaagtgca tttgaagaaa atgggaagaa aggtgtagta gaacttacca
    41881 actataaagt aggagataca gtaaattgtg aatatcttga tgaaaatgcc ttaagtaaaa
    41941 aagtaaaggt caagattctt gctataactg acgaagaacg tctaggtctt ggttatcaaa
    42001 atatggggtt acaatttata acttatgatg aagttgctaa aaatttaaat ttaaaattaa
    42061 gtaaaagcct tatatttatt gattcaggag gaagcattaa gactaagaag gaaatagaag
    42121 ctttggcaaa taaaaataat tttaactttt atgatgaaag tgcaagcaat gaaagtgaaa
    42181 aacaagattt aaaggttatt aaaatatttg tatatggttt tatagtagtg atatctttag
    42241 tgagtgttac aaatattcta aatacagtaa gtacaagtat aaatctaaga aaaagagaac
    42301 ttgcagtaat acaatctata ggggttactc ctaaaggatt taggaaaatg atatatttag
    42361 aaagttttat atatggtata ctgtcattgt tgtttggaat accaattagt attggtataa
    42421 ttctaattat gaataaactt atttcaggtg taattgaatt ttctccgatt ataccgtgga
    42481 cagccatagt tatctgtata gtaagtgttt ttataataac ttttatagca gggtatatac
    42541 caatgagtaa attaaataaa gaaaatataa tagataatat aagaagagaa agtatataga
    42601 tataaaaata attcttaact atataaaata ttattaaaat catttaagtg gtataaaata
    42661 ttattatatc acttaaatga ttttttattt atataagaaa tctaataata aattactaga
    42721 ggtatttaac tatttagaaa gaattggtat tatataaagt aagagaatat ataataagaa
    42781 agaatatttg attttattgg aaaacaaaag aaaaagaagg tgattactat ttctcatata
    42841 actgcgaaga atatgtataa aagtttagag gaaagaataa acaagtttcc acaaggtgct
    42901 ccaccatctg atacattata caaaatacta aatgtactat atacagaaca agaagcgaag
    42961 ttagttgctc agcttccaat aaagcctttc agagtaaaaa cagcagctaa aatttggagt
    43021 gtaagtgaaa gtgaagcata tagagtattg gataaacttg ctagtaaggc tctgatacta
    43081 gatatagaag ataataaagg gaagaaatat ataatgcctc ctccaatggc tggttttttt
    43141 gagtttgcca tgatgagaac gagacacgat atagaccaaa aacttttggc agagctttac
    43201 tatcaatata tgaatgtaga agaagatttt atcaaagatt tattttattc aacagaaacg
    43261 aagcttggaa gagtatacgt tcaagaggaa gttttaacta atgataatga ggttagtatt
    43321 ttagattatg aaagggctac tcatataatt gatgaatcaa ctcatatagg tataagtatg
    43381 tgttactgta gacatagaat gcaacatgtt ggaaaagctt gtgatgctcc tatggatata
    43441 tgtatgacgt ttgacaatgt agcaaattct ttaataaata ataaatttgc aagacgtgtg
    43501 gataaaatag aatgtaaaga actacttcat caagcatatg aacataactt agttcaatgt
    43561 ggagaaaatg ttaggaaagg ggttacgttt atatgtaatt gctgtggatg ttgctgtgaa
    43621 gcaatggtag cagctaaaag atttggaaat cttcatccag tacagacaac aagttttata
    43681 ccaaatataa accatgaaaa ttgtgtaaag tgtggtaaat gtataactgc atgtccaata
    43741 gatgcgataa gcaaggttaa ggaagatggt aaagaatata taaaaattga tgaggataga
    43801 tgtctaggtt gtggagtttg tgttagaaat tgccataaaa atagcattat gttattaaag
    43861 agagatgaaa aaataataac tccagcaaat tcagtacata gagcagttct tatggcaatt
    43921 gaaaaagggc agttacaaaa tttaattttt gataataatg ctctggctag tcatagagct
    43981 atgggagcta tactgtcagc tatattaaag cttgaaccag ctaaaaagat tttagccagt
    44041 aaacaattaa agtcagtata tttagataaa ttgttaagta tgaatgataa atagaaacta
    44101 acttaaaaaa ggcaaagttt tagacttatc tatatcttaa taaacttatc atttaatatt
    44161 taaatttaat ttttacacat ataaaactta taacttaaat aaaaaaaggc tatattcaat
    44221 taatattact tgaatacagc cttttttagt tatgttaagc tatttctttt atttgcttaa
    44281 atgagttttc atttccattt ccaccaattt gtgttacaga tttaaatctc atagttttaa
    44341 atagttcttt ttgattataa tctagtgagt ttcctactag cattacagga gatttagttt
    44401 ttccagccaa aactccaaca gataatccgt ctattaaatc atcttgttta ttcattccat
    44461 ttttagcaac atataaattg tctatcttag aatccttgta aaattctttt attacttttg
    44521 cattagtttc atttctagtg ctacctgata ttctctgtgg attttgaagt ttactttcta
    44581 tatttttaga aactgtatat tctccaccga ttacataaga tttatcaatc tttttgtttt
    44641 taaacaagtt atttatttca gttatattac tattttcatt agtaagtatt ataggcatat
    44701 cattttgagc tgatacagct ccaatgctta ctgcatcggc taatcctttt tcgccattaa
    44761 ctactgaaat ctttgatata ttgctaatat tgttcaattc tttagcaatg ttcatagaag
    44821 tttgatatct atcacttcct gaaattctag atacagagat attgtttgat tttatagtat
    44881 ctaaagattt ttcatttata gaagtttctc caccaactac atatacattt ttaactttta
    44941 atctctttaa ttcagccaaa gttttttcat ttatttgaga gcttccagtc aataatatag
    45001 gagcgttttt cttgtatgca aatggtgttg cagccaaagc atctgcaatt gctgaatcgt
    45061 ttattaaaac tgcattttca gcattttccc aacctgcttg acttatctta atagaagttt
    45121 catatctatc agttcctgta agtgcacttg catttacttc atcttgatat gtaacctcaa
    45181 atctagcatt tggacttcct aagaaaacaa ttacgcctcc ttttggcaat ctaactctat
    45241 tgttctttgt aactaaagaa tagtttacag gaacaccttt atcatcataa acagcaaaag
    45301 ctgaattttg aggaagatta acttctattt ttttatttgc gatatcgtca ccaatcttat
    45361 accattttgc ataaccattg gattctagtt cacaagtaaa agatttttct gctggcaaat
    45421 tagttataga atcttcacta acatacgtta gtgttccaaa agataaatac tctgtaccat
    45481 tttctttatg aagtttaatg tcggataaat cacgacctat tacaccaggt atttctataa
    45541 aagcatttga gttattctca tccattattt tagtgttaac tatataacct ggagtttcat
    45601 cagaaagact aaaacttgct tttacagagc caaacatata gctttgagaa gtatattttt
    45661 catccactag atagtaacct ttgccacttc tttttttcca cacttctttg acactatttg
    45721 atatattgtt gtcatctact ttttgagcta catagtataa acttgctgtc tgacctaatc
    45781 cagggacatc atcataagag ctcatattta aatatgtgat attatttttt tcttttacaa
    45841 attttaaaca actattacct ttttcagata caaatctatc ttgacctatg tatacgtatt
    45901 tatcttcagg tccattttct atataaggag aagatactgt taaagtacca ttatcattaa
    45961 catctacttt tatcatattt gaagaagcat aaagaccact attttctttt aaagagctag
    46021 gcattttaac ttgttgtggc ttactaaatg ttttatcagg ttttatttct ttaattttac
    46081 ctttttcttt taatgcagat agtaagattt cttgtccaat tatttcatta agttgactac
    46141 ttccaccaga agaaagaact gcaactgcca tattttcatc tggaagaact ataagattac
    46201 tgtggaataa taaagaatct ccaccttttg taagagcttt taaattatac tgattaaatg
    46261 gataagtgtt gacacaatcc caccctaaac catatccaag tatagagtct tctccttcag
    46321 gccatagacc atttaaatat tctttgtttt ccatagcttt tactgaagca ggagaaagaa
    46381 taccatttga atttttcata aatgtttgtg caaatgtaca tagattttct gcacttgaat
    46441 ataaaccacc tgcacctata gcattaaggt tatcttgtgg aactgcatct tcccaataag
    46501 gtacatatgc ttttgctagt tttgaactat caaaactatt ttctggagtt tttgtatttt
    46561 gtaagttaag tggattattt atgtatttat caagaaaatt agtaaatgac ataccagata
    46621 ctctttctac taaaatttca gctaaagtaa atccatcatt acaataaaca gaaaaagctc
    46681 ctggtttagc ttttaatctt tgtttttgta attcttttaa gaaattatca tgaccataag
    46741 aatcattgtc tgctaataaa atagtgtttt taaaactact tcccatcaac cctgatgaat
    46801 gatttaataa cattcttgga gtgatttcct tgtatctatc atcagccatt ttaaattcag
    46861 gtatatactt tacaacagga gtatctaaat ttaattttcc atcatcaacc aacttcataa
    46921 cagctgttgt agtaaacatt ttactgatag atgcgatact atacatatta tccttattta
    46981 agtttttgtt atcctgtttg ctgtaaacgc caccattacc agaaatctca attttgccat
    47041 tatcaatcaa agcatattga gcacttacag caccatactt tgatagcaaa gtttggattt
    47101 tctctttagc aagagcttta gtttttgtgt aagattccgc gtttttacta taatcatgta
    47161 attctatatc agaactttga tatttttcgg aattatcttt tgtattatca gcaaaagtaa
    47221 tatttggagc agcagtaaat aacatactca atactaatgt actagataat aatttagaga
    47281 gcctttgctt aaacatatta cattacctct ttcccttttt aataatttat aattagcata
    47341 acattcttaa ttattttaat caacagatta ttatcaat
//
LOCUS       DAELLV010000007        44341 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.007, whole genome shotgun sequence.
ACCESSION   DAELLV010000007 DAELLV010000000
VERSION     DAELLV010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 44341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44341
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.007"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            314..1510
                     /locus_tag="KPD58_000345"
     CDS             314..1510
                     /locus_tag="KPD58_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBG0264136.1"
                     /translation="MKILVLNCGSSSLKYQLIDMNNEEVLCIGLVERIGIEGSILKHE
                     KAGRDDKYVVEQPMKDHKDAIALVLEAVAHPEFGAVKEMKEIDAVGHRVVHAGEKFAT
                     SVVITPEVEEALKECIDLAPLHNPANIMGIDACKAILPDVPMVGVFDTAFHQTMPKSS
                     YLYGLPHELYTKYGVRRYGFHGTSHNYVSQRAAEILGKDIKDLKIVTCHLGNGASIAA
                     VDGGKCVDTSMGFTPLEGLIMGTRCGDIDPAILPFLMRKEGLDADGLDKLMNKESGVY
                     GMTGISSDFRDIEDAAKNGDERAQATLEAYVKKVQKYIGAYAAEMNGLDVVVFTAGVG
                     ENGKAIRADIASNMEFLGMKLDKEANDVRGKETVISTADSKVKMLLIPTNEELMIARD
                     TLRLVK"
     gene            1603..2127
                     /locus_tag="KPD58_000346"
     CDS             1603..2127
                     /locus_tag="KPD58_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HBG0264137.1"
                     /translation="MKVSIEKINRKETDKIDLNFCEKIDTISYCDEIYKLVSPVNLKG
                     KVSKTNKGLYLDIDVNFTIVDNCSRCLKEVEIPLEYSIQGFLVKEEDYDEDEFEEFDP
                     FIFDGEEIDLIDIIEQTLDFNVPHKVLCSENCKGLCQVCGANLNEEECSCSEITNDEE
                     YIDPRFAKLKDLFN"
     gene            2152..2328
                     /gene="rpmF"
                     /locus_tag="KPD58_000347"
     CDS             2152..2328
                     /gene="rpmF"
                     /locus_tag="KPD58_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBG0264138.1"
                     /translation="MAVPKRKTSKSNTKMRRAANSKMEATGFVSCPQCHEPKLPHRVC
                     PDCGYYKGKEVVSK"
     gene            2535..3092
                     /gene="fapR"
                     /locus_tag="KPD58_000348"
     CDS             2535..3092
                     /gene="fapR"
                     /locus_tag="KPD58_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HBG0264139.1"
                     /translation="MKKKSKAQRQKELIDMLKTDPFYTDEELSSLFDVSIQTIRLDRM
                     SLNIPELRERVKSIAETQSSKVKTLGVKEITGEIIDLSVGRLGISMLEVTQDMIYSKT
                     NTLKDTYIFSLADSLAMAIIDAPKVIMRVANVKSFKLIEQQDRLIAKAEVYRNIDKKH
                     YVKVVINNKAQEQIFRGKFIFEELD"
     gene            3103..4125
                     /gene="plsX"
                     /locus_tag="KPD58_000349"
     CDS             3103..4125
                     /gene="plsX"
                     /locus_tag="KPD58_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBG0264140.1"
                     /translation="MKIVIDGMGGDNAPKSNVEGAVNAIKEYQVDLIITGDKDLLEKE
                     FSNYEFDRNKLEIVHTTEIIENEDKPVKAIRSKKDSSMVVALNLVKEGKADAIISAGN
                     TGALLAGGLFVVGRIKGIDRPCLCSAIPNVKRGMTLIADCGANADCKPKNLVEFAAMS
                     NIYSRKVLGLENPKVALANVGLEEGKGNDLVKRSYEEIKKLDLNFIGNVEAREVINAY
                     TDIIICDGFTGNILLKSAEGVALSVMSLIKETFMASTKSKIGALLIKDDLRKLKSFID
                     YSEYGGAPLLGLNGGVIKAHGSSDAKAIKNAINQGIKFSKGKVVEDINQFISKYNEEN
                     KNNEDE"
     gene            4151..5137
                     /locus_tag="KPD58_000350"
     CDS             4151..5137
                     /locus_tag="KPD58_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HBG0264141.1"
                     /translation="MNTKAGILGVGSYLPEQSYDNFHFEKIMDTSDEWISTRTGIKER
                     RFAKESEATSDLASKAALKAIECAKLNVEDIELIILATITPDMSLPSTACIVQDAIGA
                     VNATAFDISAACSGFVYGVTIAKQFVETGCYKNVLVIGAETCSKFLNYDDRTTAVLFG
                     DGAGAAVIGPVNEGGILSTHMGSDGKGKDCLKVPAGGSRLKASKETVEANLHTIEMAG
                     SDVFKFAVRKMAETSLRALEKANLNTTDIDYLVPHQANIRIIQASSKRLELDMKKVYV
                     NIDKYGNMSAASIPVALDEAYREGKIKKGDNVVLVGFGGGLTWGASVVKWTL"
     gene            5246..6175
                     /gene="fabK"
                     /locus_tag="KPD58_000351"
     CDS             5246..6175
                     /gene="fabK"
                     /locus_tag="KPD58_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HBG0264142.1"
                     /translation="MNKICKILNIKYPVIQGGMAWVATASLASAVSNAGGLGIIAAGN
                     APKEAIKKEIVECKKLTDKPFGVNVMLMSPFVDDIIDLIIEEKVQVITTGAGNPAKYM
                     DRLKEAGTKVIPVVPTIALAQRMEKLGATAVIAEGTEGGGHIGELTTMVLVPQVADAV
                     NIPVIAAGGIVDGRGIAASFALGASAVQVGTRFICSEECSVHSNYKNLVLKAKDRDAI
                     VTGRSTGHPVRTLKNKLSKEFLKMEQNGATPEELDKKGTGALRFATVDGDIEKGSFMA
                     GQSAAMVKEITPCKEIIEAMVNQAREIMPAIEL"
     gene            6191..7141
                     /gene="fabD"
                     /locus_tag="KPD58_000352"
     CDS             6191..7141
                     /gene="fabD"
                     /locus_tag="KPD58_000352"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBG0264143.1"
                     /translation="MGKVALVFPGQGAQYVGMAKDLYENNTVAKSVIDEASDALNMDL
                     KNLMFNGNEEELSKTENTQPAMVTHSVAVLKAVQAQIDLKYNACLGLSLGEYSALVAA
                     DAIDFKDAVCLVKKRGKFMQETVPAGVGAMAAILGLDRNVLENVVKDIQGGIVEVANY
                     NSPGQIVISGENEKIEEAMVKCKEAGAKRAVKLNVSGPFHSSMLKEAGVKLASELEKV
                     NITKPKVDVVANVNADYYKNEGKDLLIKQVSSSVLWEDSIERLLNDGYDTFIEMGPGK
                     TLKAFIKKIASNKKANVNIYNIDGIDSLNEFVQNYRNGEI"
     gene            7141..7890
                     /gene="fabG"
                     /locus_tag="KPD58_000353"
     CDS             7141..7890
                     /gene="fabG"
                     /locus_tag="KPD58_000353"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HBG0264144.1"
                     /translation="MINLTGQVAVVTGGSRGIGKEIAKKLASFGADVVINYTSKEDEA
                     LKTKNEIESMGVKCTSIKCDVSKFDEVNQMIDSVVSEFGKIDILVNNAGITKDGLLMR
                     MKEEDFDRVIDINLKGVFNCTKAVTKPMMKKKYGRIINMTSVVGIMGNAGQTNYCASK
                     AGVIGFTKASARELASRNININAVAPGFIETDMTKVLSDDVKESTLANIPKKSYGKPE
                     DVANAVAFLVSDMSSYITGQVINVDGGMVMQ"
     gene            7944..8168
                     /gene="acpP"
                     /locus_tag="KPD58_000354"
     CDS             7944..8168
                     /gene="acpP"
                     /locus_tag="KPD58_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBG0264145.1"
                     /translation="MFNKVVEIIIEQLGVEDKEITMETSLMKDLEADSLDAVEIIMAL
                     EDEFGIEIPDTEAENFKSIGDIVKYIEANK"
     gene            8197..9435
                     /gene="fabF"
                     /locus_tag="KPD58_000355"
     CDS             8197..9435
                     /gene="fabF"
                     /locus_tag="KPD58_000355"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBG0264146.1"
                     /translation="MNKRVVITGLGCVTPLGTGKEEFWSNIKSGVSGIDKITNFDAST
                     YQTQIAGEVKNFHPEEYISKKELKRLDKFAQFAIVSAKLAVEDANLDLDKVDRERFGV
                     IIGSGIGGVEAIETQHKILLEKGNKRVSSLFVPMMIGNMAAGQVSIFLGAKGPNTNVC
                     TACASGTHSIGDAFKVIQRGDADIMVAGGSEAAVTGLAFAGFCNMKAMSTRNDDPKTA
                     SRPFDKDRDGFVMGEGAGIVILEDLEHALARGAKIYAEVVGYGLTADAYHMTTPAENG
                     EGAARSMNMALKDGNVPLEEVDYINAHGTSTYYNDLYETMAIKTVFGEKAYDLCVSST
                     KSMTGHLLGASGAIEAVVCAMSIEDSFVPPTINIQEVGEDLDLDYVPNQGKEKNIRYA
                     LSNSLGFGGHNATIVLKKYV"
     gene            9661..11379
                     /locus_tag="KPD58_000356"
     CDS             9661..11379
                     /locus_tag="KPD58_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG0264147.1"
                     /translation="MNFRLPKKRLPKKIIFICIILISIILFIKYDQNGLKEREIINKG
                     KYMSESLVADKEYDEAKKVVKTSFESIPEKKYNKVYNEVWDMLKTISYVPDGTKIVID
                     SLEKMRESDVKLSDECRFNIEKRLASVYIMDNNYSKAVDLTVKSIKLAEKLGNNYEKA
                     RLDVDLASIFLKIGSYETGEKLIKDSFKIDIDEGEKNGMVRLYAIINLAEIYVEEGEY
                     DKAIEISSRIKDYKKFVNTDNYNDFDIMASIMQSNAYVGKNNIQKAKTFLEKADYLIK
                     SDRTVYILDKDMYYYMAMGNLEYKSENYNSAIDNYKKSLEISTKRKLTQEKIKNLNKI
                     LNIYEKQGNIESLNEYQKMLINEYKENKSIRDSETSFYIIDKVSNESKLFEKTKKEIK
                     YYKILFTVILIAILGSTFLYNRLKYFKAQNLHDGLTNVYNRKSFDIMYEKYREKDDNF
                     ALVMIDIDNFKLINDNYGHKFGDTVIKGITNTICVMLEQDDKVFRYGGEEFSVLIRNK
                     SGEEVADIIDSIRVAIANKKWNDDVTVTISAGVAHISDSKDVLEEADKNLYKAKQLGR
                     NKVVYK"
     gene            11410..13410
                     /locus_tag="KPD58_000357"
     CDS             11410..13410
                     /locus_tag="KPD58_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0264148.1"
                     /translation="MKQSIAIVTDKITKLSSFLEENIRLVLGNYIDINHYYLEVLKEN
                     DKIQGDFVLVMNDDRLNSMRKHLVQDSKIIVIRRTLKENEIYGLFSIPNGTEVLVVND
                     TKETTLETISLFYKIGVKNLKFTPYIEGKIYKNIDIAITPGVKGKVPSYINKVIDLGN
                     RYIDISTFIEIINGLRIDVKEIRKNLMAYSESLISLDNGVKDNYRQLFLKIEEQDVIL
                     NLSKDGIIFTSADGIINTFNKEALKILGINENPQGKNIEDIISEDLNILLEDKEIIDE
                     VVFTNKKYINVNKKSIFSMGNKAGIYYNLQEITYIKKLEQNLTNKLREQGQIARYTFK
                     DIKTKNKNMVKCVELAKKISKSDLSVLIIGESGTGKELLSQSIHNASNRSNQPFIAVN
                     CAAVPDSLLESQLFGYEKGSFTGALREGKKGLFELANNGTIFLDEIGDMPPLLQTKLL
                     RVLQEKQVMPIGSHNVINIDVRVIAATNKNLLKMIREGKFREDLYYRLNVLPIDVPSL
                     RNRKEDIITLMNFFLKNNIRLSPEVITILEKYNWPGNIRELQNVASYVSLMCDSMVFR
                     DDLPPYIRVECANNYEDGFGYTRKEFEEILRILYERRESSSGIGRGFIIEKLIQEGIE
                     ISESKVRKVLSYLSECGYISSKSGRGGSQITSEGIKLYNNIN"
     gene            13761..14006
                     /locus_tag="KPD58_000358"
     CDS             13761..14006
                     /locus_tag="KPD58_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HBG0264149.1"
                     /translation="MKYKTTGTCATEIEFEVKENKVTNVNFIGGCDGNLKGLKVLVEG
                     MNIEDVIQKLKGIECKTKPTSCPDQLSLALENYMNIK"
     gene            14041..14766
                     /locus_tag="KPD58_000359"
     CDS             14041..14766
                     /locus_tag="KPD58_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBG0264150.1"
                     /translation="MKPIIGILGNLIIMENGMFPGLERSYVNNDYINAVLKGGGSPVI
                     IPVNTDKEVIKKQIEMVDGVLISGGWDINPQLYGEETREETTFIYPEVDEFDLIAISI
                     ALELKKPILGVCRGLQILNVSLGGTLYQDNNLIEGSYIKHTQSSKRHVATHKVDVKEG
                     SILEGILGKQLLTNSYHHQSVNQLGKGLKAIAYSKDGIIEAIEKEDENFVVGVQWHPE
                     MMVDYCDKMEKLFKYFINICSNL"
     gene            14788..16101
                     /locus_tag="KPD58_000360"
     CDS             14788..16101
                     /locus_tag="KPD58_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG0264151.1"
                     /translation="MNEKNKMGLISIILLGINAVVGAGVFLLPGDAMKSFGVASIFVY
                     IFDMLLVLSMAFCFAEVAGKFNKNGAAYVYTKEAFGDFCGFEVGLMKWVIGCISWGAL
                     IVGFPTSLSAVWAPAGEPHIQKIIIVAMIVGLTIINLLGVSLSKIVQNVITVGKLIPL
                     ILFIGIGIFFIKGVNFTSSTMVPPGAGATEFGAAALLMFYSFTGFESIAVAAEDMENP
                     QKNIPIAIISVIVIASIIYILNQVVCVGILGDSLSSTSTPVADAARICFGNMGAGLVT
                     FGTLVSVGGICMCGAFVNPRSCVALADDKMLPRIFARKDKKGTPYVAIIATMLITIPI
                     ALSGSFAELAAISAVARFIQYIPTSLSVLVFRKKRPELVGTFKTPFGAVIPLIAVCVG
                     TWLLFQASMHQLIMGLGALAIGVPLYFIMKSYNRKIYGENLKKIV"
     gene            16307..16846
                     /locus_tag="KPD58_000361"
     CDS             16307..16846
                     /locus_tag="KPD58_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0264152.1"
                     /translation="MPTITLKNGVDVLIREGVREDAQSIIDFYNEVGGETHFLSFGKD
                     EYKISLEEQENAIESAKASDNSVKLIAFIDGEIVGIATIDSNQKAKGKHVGVLGIVVK
                     EKYWGIGLGKRLMLDLIEWCKSNGITKKITLVTNEENYNAIGLYKKVGFEVESILKKE
                     CYYNGVYTDLIGMSLLLGI"
     gene            16935..18920
                     /locus_tag="KPD58_000362"
     CDS             16935..18920
                     /locus_tag="KPD58_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HBG0264153.1"
                     /translation="MKNLCKISDDYLNSKLKYLKLLSKQYPSISKASTEIINLEAILN
                     LPKGTEHFITDVHGEYEPFVHVLKNGSGVIKRKIEELFSNTIRDSEKKMLATLVYYPE
                     QKLDLIIKQEENIDDFYRINIYRLIELCKYASSKYTRSKVRKLLPENFKYIIEELLHE
                     HVKSEHKEEYYKSIVETIVDIGIAKEFIIAISTVIQKLVVDRLHVIGDIYDRGPRPDI
                     IVDKLIEHHCVDIQWGNHDILWMGAASGEKTCIANALRISARYANLDIVEDIYGINLL
                     PLATFAIEMYKDDPCKEFIPKVNDQSVTTTEKSLMAKMHKAISIIQFKLEGEVIRRRP
                     EFEMEHRLLLNMINYDEGTINLKGKTYKLKDTYLPTIDKKDPYKLTMEERNVIDKLVS
                     SFRGSEKLQKHVSFLFSKGSIYLKANSNLLIHGCVPLNEDGSFMSMNIMGKEYKGKAL
                     MDKMESLAREGFFFKDKAEEKLYGMDIMWYLWTGKCSSLFGKDDMTTFERYFIAEKET
                     HKENKNPYFKLRENEMACKRLFEEFDLELDESHIINGHVPVESKNGESPIKANGKILV
                     IDGGFSRAYQKTTGIAGYTLIYNSRTLQLVSHEPFNSAEEAIANESDILSTTVVVEHK
                     AKRKMVRDTDEGIKIQEEIEDLKLLLMAYKKGLIKEM"
     gene            19144..20112
                     /gene="spoIIIAA"
                     /locus_tag="KPD58_000363"
     CDS             19144..20112
                     /gene="spoIIIAA"
                     /locus_tag="KPD58_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HBG0264154.1"
                     /translation="MNKLSDEIINSLSTTIREKVEKVSNNNLNIEEIRLRSQKPLILN
                     ANSKDYFYNQKTMTLDLNQQNSYVVTREDVEQTFQIICKYSIHSFMDDIKKGFITLRG
                     GHRVGLVGKAIVEDGQVKNIKHISSLNIRVSREIIGCSDKILSHIIKGKNQINNTLII
                     SPPQCGKTTLIRDIVRNLSNGNEDYGFKGLKVALVDERNEIAGAYLGVPQMDVGIRTD
                     IIETCPKDLGITMLLRSMSPNVIVTDEIGSEKEIKALYTALNGGIGLITTVHGDSIED
                     IQNRKELNRLLDKELFKKVIILSAKRGAGTIEKIYDLEEKRWYFAN"
     gene            20102..20623
                     /locus_tag="KPD58_000364"
     CDS             20102..20623
                     /locus_tag="KPD58_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HBG0264155.1"
                     /translation="MQIKIIIIAFLIGSSYLIGEQIYKTYTRRHKQLNDLIRVLEILR
                     MDLSFGLYTLEEIFNRIGGNKEFCFWKFFYQISEGLHNEQSKTLEIIISENIDVLSKE
                     TYLGNKEIEELKNLILTLGKSDIESQQRMIDLSIENLKKQTYETKEDINKKGVLYKKL
                     VTFIGIGICIILI"
     gene            20640..20837
                     /gene="spoIIIAC"
                     /locus_tag="KPD58_000365"
     CDS             20640..20837
                     /gene="spoIIIAC"
                     /locus_tag="KPD58_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HBG0264156.1"
                     /translation="MEISLILKVAGVGILISVLNMILEKTDRKDWAGLTTLAGVIIVL
                     GMVITEISDLFNTVRTMFQLY"
     gene            20853..21239
                     /gene="spoIIIAD"
                     /locus_tag="KPD58_000366"
     CDS             20853..21239
                     /gene="spoIIIAD"
                     /locus_tag="KPD58_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HBG0264157.1"
                     /translation="MEIMQLIGIAIISTTLCLVIKKDRPEIANFIAIITGVIILLSVM
                     FKLNFIVDSIQDLANKANIPTMYISLIIKLIGIAYLMEFAIQLCKDCGEGNIASKLEF
                     GGKIIVMSMSFPILLSIVEMVVNIIP"
     gene            21324..22529
                     /gene="spoIIIAE"
                     /locus_tag="KPD58_000367"
     CDS             21324..22529
                     /gene="spoIIIAE"
                     /locus_tag="KPD58_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HBG0264158.1"
                     /translation="MKKRFLPMIIGFLFTFFFVNIFAIMIFANEVPSSEDKEYGETKN
                     SIDKYIDGQLDKLDINEIQDYINKEIVINDVNLKSFVKDLISGEKNILDLFNKDGLKI
                     LMFDEFKASLKVVAVILVLALLSSILKSLENSFSSGAVSQIATYIIFITMVSLTLIGF
                     KDVLQICYDAIDHTVGLMQVIMPILITFLLLIGFPITSTTLNPIFIGGVTFINVFFKN
                     FLFVSITVAFGILIINNLSKNIRLKRFFSFVKQINYVSIGAMFTVYLGLVSIQGLYVT
                     SFDKFSVKTAKFAIGNFIPVVGGFVSDSVDILLSSSQLIKNIFGGIGLILLVGICLLP
                     VIKILSVIVVYKLAAIIVEPVGEDGISNFLNEVANLMIIMLASVIAITVMFFVTVAIL
                     TSISVVSQG"
     gene            22589..23206
                     /locus_tag="KPD58_000368"
     CDS             22589..23206
                     /locus_tag="KPD58_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HBG0264159.1"
                     /translation="MLEGIKAWIVSVLIGAFIVNIVDMILPSSKIKPYVNLVLNFMFV
                     FIVITPVVGFFSKDMSLEDRILKSMGNYNKQYVDSTNALAKETGNNSLSKGYEDGLKE
                     VLKLKLDEYGYDLEDIELNGANINNIKIKEKNNSAKSNNSSNEENKNNSTKREEENIN
                     SNDKENSKQVFKKGTEYGLNLNEEKLKNDLIKVLDVSIEDIQIDK"
     gene            23218..23772
                     /locus_tag="KPD58_000369"
     CDS             23218..23772
                     /locus_tag="KPD58_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HBG0264160.1"
                     /translation="MLKNLNDKDKRKVYSLITIAGICVVSLVLLSCFPSSKSDKQVGK
                     TETNKNAEKQVTKEQEKDDLESKLTAILSKIDGAGDVDVMVTFESSEEIQPAFNSNNT
                     TETTEEKDAQGGERTVTTSSENKTMITSNSSDPVVIKTTEPKIKGVIVVASGASDPSV
                     KETLYSAVQTSLQVAGHQVEIYSK"
     gene            23799..24488
                     /locus_tag="KPD58_000370"
     CDS             23799..24488
                     /locus_tag="KPD58_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HBG0264161.1"
                     /translation="MKFNYKGRGFVIITLTAMLVVVGTVNYQLSKKSLLETSKEFKAY
                     EEAQLQKNTDESDSSKTEDSSNIDKQDGKESADIDIVDSKASKVKEKATETSKEIKAQ
                     LSSEKNMKKASYILDMKMNREKQRNELVQDLNEMINNPSTTEESRKEASNMKLNIVKI
                     QEKELQIENLLSTKGYEEALVYISDNKVNVVVNEAKLEKKDAAKIFDLVAEQANVKYE
                     NIKLTNNNSNK"
     gene            24598..24960
                     /locus_tag="KPD58_000371"
     CDS             24598..24960
                     /locus_tag="KPD58_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBG0264162.1"
                     /translation="MEDNKFGQVKISNDVIATIAGLAALEVEGIETTATLTDKLLKNN
                     GVKIQIEEEDVNLDVMVTIKYGMSIPDTAFKVQENVKNTVETMTGLKVSQVNIHIQGI
                     SFKKDKVDKEEAKATKKN"
     gene            25045..25551
                     /gene="nusB"
                     /locus_tag="KPD58_000372"
     CDS             25045..25551
                     /gene="nusB"
                     /locus_tag="KPD58_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBG0264163.1"
                     /translation="MKKDRAQKSTTREYIMKLIYQININKEDFETLEDKVDNFLKDNS
                     EHIINRYKELALQYSKNTNLKLEDTEIEDVIDKKYINTVCKALKENHDKIDELINKHA
                     KNWTVDRMPKVDVSILRLSVCEILYLDTPNKVSINEAVELAKIYCDDKSPKFINGILG
                     SVVDEIGK"
     gene            25535..26605
                     /locus_tag="KPD58_000373"
     CDS             25535..26605
                     /locus_tag="KPD58_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-sialoglycoprotein endopeptidase"
                     /protein_id="HBG0264164.1"
                     /translation="MKLGNNIIIGIDTSCYTTSIAAISLDKKVIFNEKIMLEVRDNSK
                     GLRQSEAVFQHINNLGILSDRIKSFKDKFNVEGVCSSKKPRPVENSYMPVFNVGHNFG
                     KLLSSIYGCRFYETTHQENHIEASLLNSKLKNNNKFISVHMSGGTTEILLTSKQDSHH
                     NVCDTNLGKIAKISIKKDDKSKLYNNFGYNIDIIGGSKDISFGQLIDRVGIKLGYKFP
                     SGKYLDENALNCNLKIESGLKTSVRDGYMNLSGLENQVNKIINDNGYNTNQKEYISKL
                     VLDSVVRNMFKSLVYLCETYNVNEVVFAGGVSASKYILRELSVKLRKKHIEAYFTEPQ
                     YSTDNAVGCAIIGLNNFLGERV"
     gene            26602..27807
                     /locus_tag="KPD58_000374"
     CDS             26602..27807
                     /locus_tag="KPD58_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBG0264165.1"
                     /translation="MKLRALDISEANSYIKRILINDPILSNLKVKGEISNFKVHSSGN
                     VYLSLKDETSKLNCVIFKSNFNRNLKLDNGVKIIANGYISVYERDGAYQLYINEIEIE
                     GIGNLHIEFNRLKEKLNKEGLFDPKYKIPIPKMPNSIGVITSPTGAVIRDIINVIKRR
                     YPKVNIKLYPVTVQGDKSAEEICEAIRFFNHMKNVDTLIVGRGGGSIEELWSFNDEMV
                     AREVFNSQIPIISAVGHETDFTICDFVSDMRAPTPSAAAEIATPSLDDINYKLGNIKS
                     RMSKSLTNQIELDQYRLETVFNKINNYLDSYTIKDKVIQLDKIYDKIIFGIENNLKLE
                     DEKLVKIGALLHNLSPLATMDRGYSITQKNGKVINSIKGLKIKDSIDIVLKDGNLECM
                     IDKIENKEG"
     gene            27809..28024
                     /gene="xseB"
                     /locus_tag="KPD58_000375"
     CDS             27809..28024
                     /gene="xseB"
                     /locus_tag="KPD58_000375"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBG0264166.1"
                     /translation="MNLTYEEAYKRLESILSELESKNASLDESLSLYEEGISLYKHCN
                     KLLDDAKLKISKFNQLGIEEDFKIEEE"
     gene            28027..28914
                     /locus_tag="KPD58_000376"
     CDS             28027..28914
                     /locus_tag="KPD58_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBG0264167.1"
                     /translation="MEFKQCLKEKASFVEKVLKEYMPKEEGYQKTVIEAMNYSLSAGG
                     KRLRPILTLEACKIVGGNEDEAIPFAIAIEMIHTYSLIHDDLPALDNDDLRRGRPTNH
                     KVYGEAMGILAGDALLNYAFEVMLAGSINKENPEKYLKAINEIAKGAGIYGMIGGQVV
                     DVESENKQIEKEKLDYIHMNKTAAMMVGCMRAGATIGGANSEQMEEITKYAKNIGLSF
                     QIVDDILDIVGDEAKLGKKVGSDIENHKSTYPSLLGLDKSKEIAHNLIDEAKKSIEKL
                     SDDVDFLKGLAEYIIDREY"
     gene            28944..29381
                     /locus_tag="KPD58_000377"
     CDS             28944..29381
                     /locus_tag="KPD58_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HBG0264168.1"
                     /translation="MDFFSEIFNNGALGISLIACFLAQFIKIFTGKEKRIELSRILIS
                     GGMPSSHSSFVTSLATVVGIEKGFNSTDFAIITVLALIIMYDAAGVRRAVGKQATILN
                     QMVADIQHGKHIEQKKLKELIGHTPLEVWFGALLGIVTALILM"
     gene            29415..31280
                     /locus_tag="KPD58_000378"
     CDS             29415..31280
                     /locus_tag="KPD58_000378"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBG0264169.1"
                     /translation="MYKYLDKVNSPKDIKNMSIEEMDLLAKDIRKFLVKSVSKTGGHL
                     ASNLGVVELTLALHKVFDSPKDKIVWDVGHQSYVHKIVTGRKDCFVSLRQFNGLSGFP
                     KENESPHDIFDTGHSSTSISIATGIACARDIKKENYSVISVIGDGSITGGMALEALNQ
                     LGYIDTNMIVILNDNEMSIDKNVGGMSKYLSSIIRNSTVEKMTDEVDKILNVTQTGEI
                     LSKTAHRFKDKLMYSFSPQDCSFFDSLGIRYYGPIDGHNTKELIDILRKAKHKKGPVL
                     LHVITKKGKGYRFAEEQPDKYHGVSKFDIKTGVTSAKVKSMSISVGEKLVDMANSNEN
                     IVAITAAMPSGTGLNLFESAYPKRYYDVGIAEQHATGFAAGLAKNGMKPYFAVYSSFL
                     QRAYDQVIHDVCITKKPVTFLIDRAGLVGNDGETHHGMFDLSYLNSIPNIVVMAPKDT
                     REMELMMDLSLKLDCPLAIRYPRGSSYYLDKGEYGEIVLGKYEVLDDGQDTVILCIGS
                     MVKHALEAKEILSREGINPTIVNARFLKPIDEGMLKALLKNHKNVVTIEDNIVTGGFG
                     SRINKFIIDNEYNVNILNIAIPEEFVKHGNIDELYDFVGLSPKSIADKIRKLVIE"
     gene            31413..32225
                     /locus_tag="KPD58_000379"
     CDS             31413..32225
                     /locus_tag="KPD58_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family rRNA
                     (cytidine-2'-O)-methyltransferase"
                     /protein_id="HBG0264170.1"
                     /translation="MKKRIDLLLVEQGYFESRERAKKAIMAGLVFVDNQRCDKAGTEV
                     KEDCSIEVKGNPIPYVSRGGLKLEKAMKNFDLTIDGKVCMDIGASTGGFTDCMLKNGA
                     IKVFSIDVGYGQLAWKLRQDDRVVCMERTNIRNVTIEDTKQFADFASIDVSFISLKLV
                     LPKAKELVRHDGEVVALIKPQFEAGREKVGKKGVVREKSTHIEVIKMISDFSVENGFE
                     ILGLDFSPIKGPEGNIEYLIHLRNGNEGYEFDGETYNNKIVEVVEASHNLDK"
     gene            32245..33924
                     /gene="recN"
                     /locus_tag="KPD58_000380"
     CDS             32245..33924
                     /gene="recN"
                     /locus_tag="KPD58_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBG0264171.1"
                     /translation="MILELYMKNCALVEELRLNIDKNLNILTGETGSGKSIIIDALGL
                     CLGDKYDRSFLRKGTDKGLVEAVFFSDNRYLKKILEENDISMEDDNLLVITRLIYSDG
                     KSTARVNGRTVKVSLLKEIASTLIDIHGQHQNQALFNKDTHLKFLDLFGENELEEFKI
                     AYKNVYHKYSEVKKALNCLTENKDEMQIQREIDLLRFQINEIEAANLNKNEYEDLLKQ
                     REVYRNSEKIYNNLNSSYSKLHNGEYNVIDLIGLASKELNDISKYDSVLSEYSDTVER
                     IMYELQDISGEIRNYKDNIDFEPYELEQIELRIDEINNLRRKYGDSIDDIFEYYGKIK
                     DRLDEILNRDERVEQLRSKLMNIEEDLKIKASKLTKARIEVATTLEKILLDELKSLNM
                     KNVMFKVNFEKGPFTLDGVDDIEFMISFNLGEDIKPIYKVASGGEMSRFMLAFKTILA
                     DIDDIDTLVFDEIDTGISGIAAQIVGEKLSDIAKKKQIICITHLPQIAANADTHYCIE
                     KDTSNNRTFTNVSKLNESQRKNEIARLIAGNNITEKTIEHASEIIELAKKC"
     gene            34138..35004
                     /locus_tag="KPD58_000381"
     CDS             34138..35004
                     /locus_tag="KPD58_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264172.1"
                     /translation="MNKKDTMHLIFTIVIFILLWYIVVAVSEINKNIESCSNKLDYLS
                     QEIMSTKSDISNTINEEMSKSYITKSIDLKVKKIEKKECVIDVDVQLSKLGKNGKVFF
                     MYKEDSDKWNETEMTKHGELSYACEIKINTGSEYDYKVVTSGAISESSDVQKLTKSDY
                     MPEQPIFNSGIRDNREYFIEIVENSNLFNHESEKSKNKVYDNIRLEKVDIIVNEGKKD
                     TIYKAKLAEGLDDGKTNNSNRNQVNYEVSFPKRDIDDIIYIKAKLTYNNGVEYTKDIT
                     EDITMGLQDLEK"
     gene            complement(35097..35588)
                     /locus_tag="KPD58_000382"
     CDS             complement(35097..35588)
                     /locus_tag="KPD58_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0264173.1"
                     /translation="MADKMAQNITLQFVEEKDLENLENVKLLFTEYSNSLNIDLCFQD
                     FNNELKTLPGKYKKPSGSLILAFVDENLAGCVALKKLEGKICELKRLYVRNQFRGLKI
                     GKILLEEIIEEAKKFGYTHMRLDTLPSMKSAQGLYEKFGFYDIEPYTYNPIEGARYME
                     LKL"
     gene            complement(35649..36089)
                     /locus_tag="KPD58_000383"
     CDS             complement(35649..36089)
                     /locus_tag="KPD58_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0264174.1"
                     /translation="MKLNVRIADINNWHDLVSLSVDKSQLDYIESNALSIAESKFITA
                     WVPVGIYDENSLIGFAMYGRLEDDRVWLDRFMIDSKYQGKGYGKASLDFLVNHLKNEY
                     NCDELYLSIFEDNKMAIKLYKDFGFEFNGELDYGGEKVMVLKSN"
     gene            36250..37308
                     /locus_tag="KPD58_000384"
     CDS             36250..37308
                     /locus_tag="KPD58_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HBG0264175.1"
                     /translation="MNKINKNFKNIWIILGAVSIFTMVIIFNKNCNVNKETQIEAFTI
                     KNHDKKFVYPMGNIIGVKANTDGVLVLGYEEEDVDYIGGIQIGDNIVKIDNKRIKNSQ
                     DVSEILNKIKKSKVEVTFERKNKYKTEVIETKKENGKYKLGLWVRDKISGIGTMTFYD
                     PSMEKFKAIGHAIKDSDTNELLKIKQGYIYKPEQLKIVKASNEKVGKIKGDFNDSNLM
                     GNFSNNSELGISGNITENHNKEFNVANKEKQLIEVGRPQDVKIGDAVILFEDKNKNIT
                     SYDIKIESIVYDKGNYRDMVIKVVDDKLLEYTGGIVQGMSGAPIIQNNKIIGAITHVF
                     RDNPKKGYGIFIDEMIKL"
     gene            37450..38274
                     /gene="spo0A"
                     /locus_tag="KPD58_000385"
     CDS             37450..38274
                     /gene="spo0A"
                     /locus_tag="KPD58_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HBG0264176.1"
                     /translation="MGGFLVEKIKIVLADDNKDFCQVLKEYLSNEDDIDILGIAKDGI
                     EALDLVKKTQPDLLILDVIMPHLDGLGVIEKLNTMDIPKMPKIIVLSAVGQDKITQSA
                     INLGADYYIVKPFDFVVFINRIRELVSNRVTQVEPKPRPVQETQMTRSDFVKNVGNIE
                     TEITNIIHEIGVPAHIKGYLYLREAIKMVIDNVELLGAVTKELYPSIAKKFNTTPSRV
                     ERAIRHAIEVAWSRGKVDTINQLFGYTVHNTKGKPTNSEFIAMIADKLRLEHSMVK"
     gene            38687..39808
                     /locus_tag="KPD58_000386"
     CDS             38687..39808
                     /locus_tag="KPD58_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264177.1"
                     /translation="MRVEAPIKVDRKTKKLAKRVESGEIAVINHIDIDEVAANSLVEA
                     KIKLVINAAPSISGRYPNKGPGILTENNILIIDNVGEELFEELKEGETIEVVDGKIYR
                     KGKFLGAGEVLDKYEVSHQIKAAYENLAVELDRFIDNTIDYAKKEKGFILGEVEIPKV
                     KTNYANKHVLIVVRGQDYKEDLSTMLSYIEEVKPVLVGVDGGADALIEFGYTPDVIVG
                     DMDSVSDEALKKASEIVVHAYTDGRAPGLKRVEELGLDAVVFPAPGTSEDIAMLIAYE
                     YKAELIVAVGTHSNMIDFLEKGRKGMASTFLVRLKIGSKLIDAKGVNLLYRSKLKIKY
                     IWALIATALFPVLVVASLSPGVQQFIQLMQLKFRVLLQM"
     gene            39826..40701
                     /locus_tag="KPD58_000387"
     CDS             39826..40701
                     /locus_tag="KPD58_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HBG0264178.1"
                     /translation="MHINMKYYIVTIGAIFIALGIGMLVGFNLNNNQQLSEQQANIIN
                     DLDDKFDILKEKNDKLDSDLASVNRDYEEAVNFINKNVDKILAGSLNGKSIGIISANE
                     NDDYTKNIEDIINKSNGSIAFNIILKENITNPEKLKEMSTKLGTDVKNANDAVNYIID
                     TLKKEDASDILTYLQELDVIKFNFIGDTYLKYDSVVIAGGNDAKDSAKQFEKIEKFVV
                     SKLKSENKYLVEVQNTGVKTSYVELYSKNKVATIDNIDEGIGSISLAILLQQGNIVGN
                     FGRLDTATSLLPSIK"
     gene            40725..41417
                     /locus_tag="KPD58_000388"
     CDS             40725..41417
                     /locus_tag="KPD58_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG0264179.1"
                     /translation="MDSYISIIIPAYNEESRIKYTLESILDIKQINEIIVVDDGSIDN
                     TAKVVSELENEKIKFFKLDKNRGKGYALNYGIKLAMKNADIIGFLDGDLGSSAKEVEK
                     LIVPILNGEADVTIAKFPPAKRKGGLGFVKGLAKNSVFEMTGVELDATLSGQRIFKRE
                     VLEKFDEIPFGYGVEVGMTIDILKYGFKIKEVLVNMTHNETGRNLKGFIHRGKQYYHI
                     KKVLGQKKKEWR"
     gene            41425..42249
                     /locus_tag="KPD58_000389"
     CDS             41425..42249
                     /locus_tag="KPD58_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBG0264180.1"
                     /translation="MKDYILYTILLLTGLFGTYAVIPLFKNLLINGNVLRPNYKKDMI
                     PVSMGIVFLPMIVINGIILAFFTTEFKDLAYIFMFMFGMISMFFAGILDDVIGNRDVS
                     GLKGHFKSLFKGSLTTGGFKALFGGFVGLIVSVAISKDIIDIIVNTLIIALSTNLMNL
                     LDLRPGRAIKAYLFIMVIIFLTLTGFVQVLPLLIVPNVLAYFNYDLKARAMMGDTGSN
                     VLGISIGMLISFGYPFNIRLGWLIFVVIMHIFTEKYSLTKIIEKNKLLNFIDKLGR"
     gene            42254..43321
                     /locus_tag="KPD58_000390"
     CDS             42254..43321
                     /locus_tag="KPD58_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HBG0264181.1"
                     /translation="MISKRVGIVESIVSQTETLDDIRVNINGEIQKAYNYPKISGTIN
                     IGDEVVLNTTAVELSLGTGGYHFVITNLNNIESTLTEGGHIMKLRYTPLQIKVDSVEE
                     QESIYHDKFANFKGLEGLSVVVGTLHSMLTPFAASFKRNNPNKKLVYIMTDGASLPIY
                     LSKNVDTLKEKKLIDSTITIGNAFGGDYECINIYTALITAKEILKADAVFVSMGPGIA
                     GTGTKYGFTGIEQGSILDAVKKLEGRAIAIPRISFADKRERHQGISHHSMTVFKEIVN
                     VNVDIPITIYDDEKLNFIKEQINLNGLDKKHNIIYIDNNKTKEDLDYFNLKVKSMGRN
                     YEQDEAFFKAASTAAYYLMEV"
     gene            43323..43859
                     /locus_tag="KPD58_000391"
     CDS             43323..43859
                     /locus_tag="KPD58_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG0264182.1"
                     /translation="MVLEEKTISSDRVYTGKVITLKVDTVEIPGQGYQKRELVEVGGA
                     VGIVAITDDNKVVLVKQFRKPIEKPIFEIPAGKLEKNESPKECAERELKEETGYSAKN
                     IKLIHKFFTSAGFSNEIMFVYLATGLTPGENNLDADEFLDVYEIELEEAYNMVLKNDV
                     EDAKTSIGLLLVKDMFKN"
     gene            43927..>44341
                     /locus_tag="KPD58_000392"
     CDS             43927..>44341
                     /locus_tag="KPD58_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264183.1"
                     /translation="MRRTYRKNDFRELNRQIIIIGLLFMMSIVVGSYINKILPGSSNN
                     ILNNINPAVEYYNLNISIKDTVIQNLKSDAIFMGSIALLSLFVVTIPAVLVAFVLKGM
                     SIGYTINSCILALKFKSIKMILIILFKNLIIIPCAI"
BASE COUNT        17972 a         4463 c         8282 g        13624 t
ORIGIN      
        1 ttattaaaat aactttatta gctctttata ttatattatt ataacaaatg ggaaagaata
       61 atctacatta agttaacttg ttaatatatt attagtgaaa tacacaattt accatactag
      121 aaatatgata taatataaaa aaaaagtata tacttttata ttaaaaggtg atatattatt
      181 tatgaaaatc gtttaatgta tacaataata tatctataac aaaataataa aagatagagt
      241 tttatttaga catttattac aaatcaaaat ttattacata ttatttataa aaaaatctaa
      301 ggaggaaata ataatgaaaa tattagtatt aaactgtggg agttcttcat taaaatatca
      361 attaatagat atgaataatg aagaagtttt atgtatagga ttggttgaaa gaataggaat
      421 agaaggttct atattaaaac atgaaaaagc aggtagagat gataaatacg ttgttgaaca
      481 accaatgaag gaccacaaag atgcaatagc attagtttta gaagctgttg ctcatccaga
      541 atttggtgca gttaaagaaa tgaaagaaat agatgcagta ggacataggg ttgtacatgc
      601 tggagaaaaa tttgcaactt cagtagtaat aactccagaa gtagaagaag ctttaaaaga
      661 atgtatagat ttagctccac ttcataatcc agcaaacata atgggaatag atgcttgtaa
      721 agctatactt ccagatgtac caatggtagg tgtatttgat actgctttcc atcaaacaat
      781 gcctaaatct tcatacttat atggtttacc tcatgagcta tatactaaat atggggtaag
      841 aagatatgga ttccacggaa cttctcataa ttatgtatct caaagagcag cagagatatt
      901 aggaaaagat ataaaagatt taaaaatagt aacttgtcac ttaggaaatg gtgcttctat
      961 agctgctgtt gatggtggaa aatgtgtaga tacatctatg ggattcactc cattagaagg
     1021 attaataatg ggaactagat gtggagatat agacccagct atattaccat tcttaatgag
     1081 aaaagaaggt ttagatgctg atggattgga taaattaatg aataaagaat ctggagtata
     1141 tggaatgact ggaatttcta gtgacttcag agatatagaa gatgcagcta aaaatggaga
     1201 cgaaagagct caagctacat tagaagcgta tgttaaaaaa gtacaaaaat atataggcgc
     1261 ttatgctgct gaaatgaatg gattggatgt tgtagtattt actgcaggcg ttggtgaaaa
     1321 tggaaaggct ataagagcag atattgcttc taacatggag tttttaggaa tgaaattaga
     1381 taaagaagct aatgatgtta gaggaaaaga aacagtaata tctactgctg attctaaagt
     1441 aaaaatgctt ttaataccaa ctaatgaaga attaatgata gctagagata cattaagatt
     1501 ggtaaaataa ttaaattaaa cctatgccta tcaagtaaaa atacttgaca aacaaatgct
     1561 agatttgtat aataaatttg gtgatatgtt gaggtgaact taatgaaagt tagcatagaa
     1621 aaaataaata gaaaagaaac tgacaaaata gacttgaatt tttgtgaaaa aattgatact
     1681 attagttatt gtgatgagat ttacaaatta gtatcacctg taaatttaaa agggaaagtg
     1741 tcaaagacta ataaaggttt gtatctagat atagatgtca attttacaat tgttgacaat
     1801 tgttcaagat gtcttaaaga agtagaaata ccactagagt attctataca aggtttttta
     1861 gtaaaagaag aagattatga tgaagatgag tttgaggaat ttgatccttt tatattcgat
     1921 ggtgaagaaa tcgaccttat tgatataata gaacagacat tagattttaa tgtacctcat
     1981 aaagttcttt gtagtgaaaa ctgtaaaggt ctttgtcaag tatgcggggc aaacttaaat
     2041 gaagaagagt gttcttgcag tgaaattaca aacgatgagg aatacataga tcctcgtttt
     2101 gctaaattaa aagatttatt taattaaatt ttgactaagg aggtggcgta aatggcagta
     2161 ccaaagcgta aaacgtctaa atcaaacaca aaaatgagaa gagcagctaa ctcaaagatg
     2221 gaagcaacag gatttgtaag ttgcccacaa tgtcatgagc caaaattacc acatagagtg
     2281 tgtccagact gtggatatta taaaggtaaa gaagttgtgt ctaagtaata gcttctaaaa
     2341 agcatgtaga gtatactcta catgtttttt ttgtacatat aaaaaaataa gataatttac
     2401 tagagaaaat aagtggattt tatgcatatt aaaaaataga aaaataataa tataagtatt
     2461 gaaaattttt gtgtgttact atatactact attagtagct ggtactaatt acagctataa
     2521 aatttggagg gaacatgaaa aagaaaagta aagcccaaag acaaaaagag cttatagata
     2581 tgctaaagac agatcctttt tatactgatg aagaattatc aagtctgttt gatgtaagta
     2641 tacagaccat aagattagat agaatgagtc taaacatacc agagctaaga gaaagagtca
     2701 aatcaatagc agaaactcag agttcaaaag taaagacttt aggagtaaag gaaattacag
     2761 gagaaatcat tgatttatct gtagggcgat taggtatatc tatgttagaa gttacacagg
     2821 acatgattta ctctaagact aatactttaa aagatacgta tattttttct ctagcagatt
     2881 cattggcaat ggctataata gatgcaccta aggttattat gagagttgca aatgtaaaaa
     2941 gttttaagtt gatagaacaa caagataggc ttatagcaaa agcagaagtg tatagaaata
     3001 tagataaaaa gcattatgta aaagttgtta tcaacaataa agcccaagag caaatattta
     3061 gaggaaagtt tatctttgaa gaattagatt aggagggaaa ttatgaaaat agtaattgat
     3121 ggaatgggtg gagataatgc acctaaatct aatgtagaag gcgcagtaaa tgctataaaa
     3181 gaatatcagg ttgatttaat aatcacagga gataaagatt tacttgaaaa ggaattttca
     3241 aattatgaat ttgatagaaa taaattagag atagttcata ctactgagat tatagaaaac
     3301 gaagataaac cagtaaaggc tataagaagt aaaaaagatt catctatggt agtagcatta
     3361 aatcttgtaa aagaaggaaa ggcagatgct ataatatcag ctggaaatac aggagcctta
     3421 cttgctggtg gattatttgt tgttggtaga ataaaaggaa tcgatagacc ttgcttatgt
     3481 tcagcaatac caaatgtaaa gcgaggaatg acacttatag cagactgtgg cgcaaatgca
     3541 gattgtaaac ctaagaatct agttgaattt gcagctatga gtaatattta ttcaagaaaa
     3601 gttttaggtc ttgaaaatcc taaggtagca ttagcaaatg ttggactaga agaaggcaaa
     3661 ggcaatgatt tagtcaaaag gtcatatgaa gaaattaaaa aattagattt aaattttata
     3721 gggaatgtag aagctagaga agttataaat gcatacacag atataatcat atgtgatgga
     3781 tttacaggta atatattact taaatctgct gaaggtgtag cactttcagt tatgagtctt
     3841 ataaaggaaa cttttatggc cagtacaaaa agtaaaatag gagcattact tataaaggat
     3901 gatttaagaa aattaaaaag ttttatagat tattctgagt atgggggagc tccactttta
     3961 ggtctaaatg gaggagttat aaaagctcat ggtagttctg atgctaaagc tataaaaaat
     4021 gctataaatc aaggaataaa attttcaaag ggaaaagtcg ttgaagatat taaccaattt
     4081 ataagcaaat acaatgaaga aaataaaaat aacgaagatg aataaactcg ttttggaggt
     4141 ttaatatata atgaatacaa aggctggaat attaggagta ggaagttatc tacctgagca
     4201 atcatatgat aattttcact tcgaaaaaat aatggataca tcagatgagt ggataagtac
     4261 tagaactggt attaaggaaa gaagatttgc caaagagtct gaagcaactt ctgacttagc
     4321 atctaaagct gccttaaaag ctatagaatg tgcaaagtta aacgtagagg atatagagct
     4381 gattatatta gctacaatta ctcctgatat gtctttgcct tcaactgcat gtatagtaca
     4441 agatgcaata ggagcagtga atgctactgc atttgatata tcagcagctt gttctggatt
     4501 tgtatatgga gtaactattg caaagcaatt tgtggaaaca ggttgctata aaaatgtgct
     4561 tgtaatagga gcagaaactt gttctaagtt tttaaattat gatgatagaa caacagcagt
     4621 attgtttgga gatggagctg gagcagctgt tattggccct gtaaatgagg gtggaatact
     4681 gtctacacat atgggctctg atggtaaagg aaaagattgt ttaaaagtac ctgctggagg
     4741 ttcaagactt aaggcaagta aagagactgt agaagctaat ctacatacaa tagaaatggc
     4801 aggtagtgat gtgtttaaat ttgcagttag aaagatggca gaaacttctt taagagctct
     4861 tgaaaaagca aatctaaata caactgatat agactactta gtgccacatc aagctaatat
     4921 aagaatcata caagcttctt ctaagagatt agagcttgat atgaaaaaag tttatgtaaa
     4981 tatagacaaa tatggaaata tgtctgctgc atctatacca gtagcattag atgaagctta
     5041 tagagaaggt aaaattaaaa agggtgataa tgtagtttta gttggctttg gtggaggtct
     5101 aacttggggg gcttcagttg tcaagtggac tttatagtat aaaatatcta aaatgattta
     5161 attaacaaaa ttaaacttat acacaaaata aagttgatta ataataaaaa gtttactttt
     5221 atgatttcaa gtgaaggaga attgtatgaa taaaatttgc aaaatattaa atataaaata
     5281 cccagttatc caagggggaa tggcatgggt agctactgca tcattagcaa gtgctgtatc
     5341 taatgcagga ggacttggca taatagcagc aggaaacgca ccaaaagaag ctataaagaa
     5401 agaaattgtt gagtgtaaaa aattaacaga taaacctttt ggagtaaatg taatgcttat
     5461 gtcgccattt gttgatgata taattgattt gattatagaa gaaaaagttc aagttattac
     5521 tactggtgct ggaaatcctg caaagtatat ggatagatta aaggaagctg gaacaaaggt
     5581 tattcctgta gtacctacaa tagctttggc acaaagaatg gaaaagctag gagctacagc
     5641 agtaatagca gaaggtactg aaggtggagg acatatagga gaacttacta ctatggtctt
     5701 agttccacaa gttgctgatg ctgtaaacat acctgtaata gctgctggag gaattgtaga
     5761 tggtagagga attgcagcat catttgcatt aggtgccagt gcagttcaag taggaactag
     5821 atttatttgt agtgaagagt gttctgtcca ttcaaactat aaaaacttag tactaaaagc
     5881 aaaagataga gatgcaattg taacaggaag aagtactggt catccagtaa gaacattaaa
     5941 aaataaacta tcaaaagaat ttttaaagat ggaacaaaat ggagctactc ctgaagaact
     6001 ggataaaaaa ggtacaggag ctttaagatt tgcaacagta gatggagaca tagaaaaagg
     6061 ttcatttatg gcaggtcaaa gtgctgctat ggtaaaagaa ataacacctt gtaaggaaat
     6121 tatagaggct atggtaaatc aagcaagaga gattatgcca gcaatagaac tgtaaatcag
     6181 gaggagcaag atgggaaaag ttgcactagt attccctggt caaggagctc aatatgtagg
     6241 tatggccaag gatttatatg aaaataatac tgttgctaaa agtgtaatag atgaggctag
     6301 tgatgcctta aatatggatt taaaaaacct tatgtttaat ggaaatgaag aagaactttc
     6361 taaaactgaa aacactcaac ctgctatggt tacacatagt gtagcagtat taaaagcggt
     6421 acaagctcaa atagatttaa aatataatgc atgcttagga ttatccttag gagagtatag
     6481 tgcgttggtt gcagcagatg caattgactt taaagatgct gtatgtttag ttaaaaaaag
     6541 aggtaagttt atgcaggaaa cagttccagc aggtgttgga gctatggcag caattttagg
     6601 tttagataga aatgtacttg aaaatgtggt taaagatata caaggaggta ttgtagaagt
     6661 agcaaattac aattctcctg gacaaatagt tatatctggt gaaaatgaaa agatagaaga
     6721 agcaatggta aaatgcaaag aagcaggagc aaaaagggca gttaaattaa atgtaagtgg
     6781 accttttcac tcatcaatgt taaaagaagc tggtgtaaaa ttggccagtg aattagaaaa
     6841 agttaacata actaaaccta aagttgatgt ggtggcaaat gtaaatgcag attactataa
     6901 aaacgaaggt aaagacctgc ttataaaaca agtaagttca tctgttcttt gggaagattc
     6961 tatagaaaga cttttaaatg atggatatga tacttttata gaaatgggac ctggaaaaac
     7021 tttaaaagca tttattaaga agatagcttc caataaaaaa gcaaatgtca atatttataa
     7081 tatagatggt atagattctc ttaatgaatt tgtacaaaat tatagaaatg gagagatata
     7141 atgataaatc ttacaggaca agtagcagtt gttactggtg gctctagggg tataggaaaa
     7201 gaaatagcaa aaaaactagc atcttttgga gctgatgtag taatcaatta tacttctaaa
     7261 gaagatgaag cactaaaaac taaaaatgaa atagaaagta tgggggtaaa gtgtacctct
     7321 ataaaatgtg atgtatctaa atttgatgaa gtgaatcaaa tgatagattc tgttgtaagc
     7381 gaatttggaa aaattgatat attggttaat aatgcaggca taactaaaga tggtctgctt
     7441 atgagaatga aagaagaaga ttttgataga gttatagata taaacttaaa aggtgtcttt
     7501 aattgtacaa aagcagttac taaacctatg atgaaaaaga agtatggaag aataataaat
     7561 atgacttcag tagttggaat tatgggtaat gcagggcaaa ctaattattg tgcatcaaaa
     7621 gcaggtgtaa ttggatttac aaaagcttct gcaagagagt tagcatcaag aaacataaat
     7681 ataaatgcag tagcacctgg atttatagaa acagatatga ctaaagtact aagtgatgat
     7741 gtaaaagaat caacactagc aaacatacca aagaaatctt atggtaaacc agaagatgta
     7801 gccaatgccg tagcattttt agttagtgac atgtcaagtt atataacagg acaagtaata
     7861 aatgtagatg gtggaatggt aatgcaataa taatttaaaa agtagagaaa tctattaaaa
     7921 taaaaaattt taggaggtac acaatgttta ataaagttgt agaaataata atagagcaat
     7981 taggagtaga agataaagaa ataactatgg aaacatcatt aatgaaggat ttagaagcag
     8041 attcattaga tgcagtagaa ataataatgg cacttgaaga tgaatttggt atagaaatac
     8101 cagatacaga agcagaaaac ttcaaatcta taggagatat agttaagtat atagaagcta
     8161 ataaatagtc gagctttaaa aaagtggagg tatccgatga ataaaagagt agtaataaca
     8221 ggacttggat gtgttacgcc gttgggaaca ggaaaagaag agttctggag taatataaaa
     8281 tctggagtat ctggtataga taaaataact aattttgatg ctagcacata tcaaactcag
     8341 atagcaggag aagttaagaa ttttcatcca gaagaatata taagtaaaaa agaattgaag
     8401 agattagata aatttgctca atttgcaata gtatctgcta aattagcagt tgaagatgca
     8461 aatcttgatt tagataaagt tgatagagaa cgttttggtg ttataatagg ttctggaata
     8521 ggtggagttg aagctataga aactcaacat aaaattcttt tggaaaaagg aaataaaaga
     8581 gtaagttcac tttttgtacc tatgatgata ggaaatatgg cagctggtca agtttcaata
     8641 tttttaggag caaaagggcc taacactaat gtatgtacag cttgtgcatc aggaactcat
     8701 tcaataggtg atgcttttaa ggtaattcaa agaggtgatg cagacatcat ggtagcaggg
     8761 ggctctgaag ctgctgtaac aggtcttgct tttgctggat tctgtaacat gaaggctatg
     8821 tcaactagaa atgatgaccc taaaactgca tcaagaccat ttgataaaga cagagatggt
     8881 tttgtaatgg gagaaggagc aggtattgtt atcttagaag acttagaaca tgctttagca
     8941 agaggcgcta aaatatatgc agaagtagtt ggatatggtt taactgctga tgcataccat
     9001 atgacaactc cagctgaaaa tggagaggga gcagctaggt ctatgaatat ggctttgaaa
     9061 gatggaaatg ttccattaga agaagtagat tatataaatg ctcatggaac ttcaacttat
     9121 tacaatgatt tatatgaaac tatggctata aaaactgtat ttggagaaaa ggcatatgac
     9181 ttatgtgtgt catcaacaaa atctatgact ggtcacttat taggagcatc aggtgctata
     9241 gaggctgttg tttgtgctat gagtatagaa gatagttttg tgcctccaac tataaacata
     9301 caagaagttg gtgaagattt ggatttagat tatgttccaa atcaaggtaa agaaaaaaat
     9361 attagatatg cactatcaaa ttctttagga tttggtgggc ataatgctac aatagtacta
     9421 aaaaaatatg tataaataaa tattaaatat aaaactaaaa aacaagcact tgttttaact
     9481 gtataaattt atattagctt aaatttaaag tctcctaaat tattttagga gactttttta
     9541 ttaagattta agtctagcat atgttgtaaa tcattttgat atattgtatt attaattatt
     9601 aagaggttat ttatatatgc aagttttcat tctacaatat acaaaaggag gactaaaatt
     9661 ttgaatttta gactaccaaa gaaaaggtta cccaaaaaga taatttttat atgtataata
     9721 cttattagta ttattttgtt tataaagtat gaccaaaatg gattgaaaga aagagaaatt
     9781 ataaataagg gaaaatatat gtctgaaagc cttgtggcag ataaggagta tgatgaagca
     9841 aaaaaagtgg ttaagacttc tttcgaatct attccagaaa aaaaatacaa taaagtatat
     9901 aatgaagttt gggatatgtt aaagactatt tcatatgttc cagatggaac gaagatagtc
     9961 attgattctt tagaaaagat gagagaatca gatgtgaaat tatctgatga atgtagattt
    10021 aatatagaaa aaagattagc atcagtgtat ataatggata ataattattc taaagcagta
    10081 gatttaactg taaagtctat aaagttggct gagaaattgg ggaataatta tgaaaaagca
    10141 agactagatg ttgatttggc gagcatattt ttaaaaatag gtagctatga aacaggagag
    10201 aaattaataa aggattcttt taaaatagat attgatgaag gtgaaaaaaa tggaatggtt
    10261 agattatatg ctattataaa tctagcagaa atatatgtag aagagggaga atatgataaa
    10321 gctattgaaa taagtagcag gataaaagat tataaaaagt ttgtaaatac ggataattac
    10381 aatgattttg atataatggc ctctataatg cagtcaaatg cgtatgtggg caaaaataat
    10441 atacaaaagg caaagacttt tttagaaaaa gctgattatc ttataaaatc agatagaaca
    10501 gtttatatat tagataaaga tatgtactat tatatggcaa tgggaaactt agaatataaa
    10561 agcgaaaatt ataattctgc aatagataat tataaaaaaa gtttagagat atctactaaa
    10621 agaaagttaa cccaggagaa aataaaaaat ttaaataaga tattaaatat atatgaaaaa
    10681 caaggaaata tagaatcttt aaatgagtat caaaagatgt taataaatga atataaagag
    10741 aacaaaagta taagagattc agagactagt ttctatataa ttgataaagt atcaaacgaa
    10801 agtaagctat ttgaaaaaac taaaaaagaa ataaaatatt ataaaatatt atttacagta
    10861 attttaattg ctatattagg gtctactttt ttatacaacc gattgaagta ttttaaagca
    10921 caaaatttac atgatggtct tacaaatgtt tataatagaa aaagttttga tattatgtat
    10981 gaaaagtaca gagagaaaga tgataatttt gcacttgtga tgatagatat tgataatttc
    11041 aagctcataa atgataatta tggacataaa tttggtgaca ctgttataaa gggaataaca
    11101 aataccattt gtgttatgtt agaacaagat gataaagtct ttaggtacgg aggagaagaa
    11161 ttttctgtat taataagaaa taaatctggt gaggaagtag cagatataat tgacagtata
    11221 agggttgcaa ttgcaaataa aaaatggaat gatgatgtga ctgtgactat aagtgcaggt
    11281 gtagctcata tttcagatag caaagatgta ctagaagaag ctgataaaaa tttatataaa
    11341 gcaaaacaac ttggtagaaa taaagtagta tataagtaat tatatactat ataaataaga
    11401 ggtataaata tgaaacaatc aatagctata gtaactgata aaataactaa actatcatct
    11461 ttcttagaag aaaatatacg tttggtttta ggtaattata ttgatataaa ccactattat
    11521 ctggaagttt taaaagagaa tgataaaata caaggtgatt ttgtactagt aatgaatgat
    11581 gatagattaa acagtatgag aaagcattta gtacaagata gtaagattat agtgataagg
    11641 agaacactta aagaaaatga aatatatgga ttattttcaa taccaaatgg aactgaagta
    11701 ttggttgtaa atgatacaaa agaaactacc ttagaaacga taagcttatt ttacaaaata
    11761 ggagtaaaaa atttaaagtt tactccatat attgaaggaa aaatttataa aaatatagac
    11821 atagctataa cgcctggagt aaagggaaaa gtaccaagtt atataaataa agtaatcgac
    11881 cttggaaata gatatattga tatatctacc tttatagaaa taataaatgg tcttagaata
    11941 gatgttaaag aaatacgtaa aaatttaatg gcatattctg agagtttaat aagtttagat
    12001 aatggagtta aagataatta taggcaactg tttttaaaaa tagaagaaca agatgttata
    12061 ttgaatctat caaaagatgg aataatattt acttcagcag atggtataat aaacacattt
    12121 aataaagaag ctttaaaaat tttaggtata aatgaaaatc cacaaggcaa aaacatagaa
    12181 gatatcattt ctgaagattt aaatatttta cttgaagaca aagaaataat tgatgaagtg
    12241 gtatttacaa ataaaaaata tataaatgta aataaaaaaa gtattttttc tatggggaat
    12301 aaagctggga tatattataa cttacaagaa ataacgtata taaaaaaatt agaacaaaat
    12361 cttactaata aattaagaga acaaggtcag atagcaagat atacatttaa agatataaaa
    12421 actaaaaata aaaatatggt aaagtgtgta gaattggcta aaaaaatatc taaatcagac
    12481 ctttctgtat taataatagg agaaagtggt actggtaaag aattattatc acaatcaatt
    12541 cataatgctt ccaatagaag taatcaaccg tttatagcag ttaattgtgc agcagttcca
    12601 gatagtctat tagaaagtca actgtttgga tatgaaaaag gctcttttac aggagcttta
    12661 agagaaggaa aaaagggtct atttgaactt gctaataatg gaactatatt tctggatgaa
    12721 attggagata tgcctccatt gctacagaca aagttactaa gggtattaca agaaaagcag
    12781 gttatgccta taggttccca taatgttata aacatagatg ttagggtcat agcagcaaca
    12841 aacaaaaact tactaaagat gattagagaa ggaaaattta gagaggacct gtattataga
    12901 ctaaatgtat tgccaataga tgtaccatca ttgaggaata gaaaagaaga tataataaca
    12961 cttatgaatt tctttttgaa aaacaatata agactttcgc cagaagttat aactatttta
    13021 gaaaaatata attggccagg aaatataaga gagttacaaa atgttgcttc ttatgtaagt
    13081 ctaatgtgtg atagtatggt ttttagagat gacttaccac catatataag ggtagaatgt
    13141 gctaacaatt atgaagatgg atttggttat acaagaaaag aatttgagga aatactgagg
    13201 attctatatg aaagaagaga gtcatcctct ggaattggaa gagggtttat aatcgaaaag
    13261 cttatacagg aaggtattga gatttctgaa agcaaagtta gaaaggtgct atcttattta
    13321 agtgaatgtg gatacatatc atctaaatct ggtagaggag gaagtcaaat aacctcagag
    13381 gggataaaac tttataataa tataaattaa gctaagtatt tgatattgaa atcacatatt
    13441 ttgaatttag atatttaaat aataataaac ttttatattt atagagatta atttatatta
    13501 tgtcagaaag aatattgtaa tttataatta aatattaata ggtttttaaa ataggattag
    13561 ataggtaata ggttaagtta gaataaccca ttacctattt ttatttgtaa gaaaagtgta
    13621 catagaatta gaagtaaaaa atacaactaa taaaactatt gaaaatacta gataaataat
    13681 aaataaaaat aattatagat tttggcatac atattgctaa ttatagagat aagacgacaa
    13741 aataattggg aggtttaatg atgaaataca aaacaacagg gacttgtgct acagaaattg
    13801 aatttgaagt aaaagaaaat aaagttacaa atgtaaactt tataggtgga tgtgatggaa
    13861 atttaaaagg gctaaaagta ttagttgaag gcatgaatat agaagatgta atacaaaagt
    13921 taaaaggaat agagtgtaaa acaaagccaa catcttgccc agaccaactg tctctagcat
    13981 tggaaaatta tatgaatata aagtaataca taaaacaatc gattagttag gagtttaatt
    14041 atgaaaccaa taatagggat tttaggaaat ttaataataa tggaaaatgg tatgtttcca
    14101 ggtcttgaaa ggtcatatgt aaataatgat tatataaatg cagtgctaaa aggtggcggt
    14161 agtccagtaa taattcctgt aaatacagat aaagaagtta taaaaaaaca gattgaaatg
    14221 gttgatggag tcttaatatc tgggggatgg gatatcaatc ctcaactgta tggagaagaa
    14281 acaagagaag aaacgacatt tatatatcca gaagtggatg aatttgattt aattgcaata
    14341 agtatagcat tagaacttaa aaagcctata ttaggtgtat gtaggggact acaaatttta
    14401 aatgtaagtt taggaggaac tttatatcaa gataataatc ttatagaagg aagttatata
    14461 aaacatacac aatcttctaa aagacatgta gctactcata aagttgatgt taaagaagga
    14521 agtattttag agggaatttt aggaaaacaa ttacttacaa atagttatca tcatcaaagt
    14581 gtcaatcagc ttggaaaagg attaaaagcc atagcatact caaaagatgg tataatagaa
    14641 gctattgaga aagaggatga aaattttgta gtaggagtac agtggcatcc agaaatgatg
    14701 gtagattact gtgataaaat ggaaaaacta tttaaatatt ttataaatat ttgttcgaat
    14761 ttataatata ccttgggggg attgaacatg aacgaaaaga acaaaatggg attaataagt
    14821 attatacttt taggtattaa tgcagtagta ggagctggag tttttctatt accaggagat
    14881 gcaatgaaat catttggtgt agcaagtata tttgtatata tatttgatat gttgttagtt
    14941 ttatctatgg cattctgttt tgctgaggtt gcaggtaaat tcaataaaaa tggtgctgcc
    15001 tatgtatata caaaagaagc ttttggagat ttttgtggtt ttgaagtagg tcttatgaaa
    15061 tgggtaatag gatgtatttc ttggggagct ttaatagtag gttttcctac atcattatca
    15121 gcagtatggg ctccagcagg tgaaccacat atacaaaaaa ttataattgt agctatgata
    15181 gttgggctta caattataaa cttattaggt gtatctctat caaaaattgt acaaaatgtt
    15241 ataactgtag gtaaactaat tccattaata ctatttatag gaataggtat attttttata
    15301 aaaggagtta attttacttc ttcaacaatg gtacctccag gagctggagc aacagagttt
    15361 ggagcagcag cacttttaat gttttattca tttacaggat ttgaatctat agcagttgca
    15421 gcagaagata tggaaaatcc acaaaaaaat ataccaatag caattataag tgtaatagta
    15481 attgcatcaa taatatatat attaaatcaa gttgtatgtg ttggtatact aggagattcg
    15541 ttatctagta catctacacc agtggcagat gctgcaagaa tatgctttgg aaatatggga
    15601 gctggattgg ttacctttgg aacattggtt tctgttggtg gaatttgtat gtgtggtgct
    15661 tttgtaaatc caagaagttg tgtagcttta gctgatgata aaatgttacc gagaatattt
    15721 gcaagaaaag ataaaaaagg tacaccatat gtagcgataa tagctactat gttaatcact
    15781 ataccaatag ctctatctgg aagttttgct gaattagcag caataagtgc agttgctaga
    15841 ttcatacaat acataccaac atctttatcc gtattagtgt ttagaaagaa aagaccagaa
    15901 ttagtaggta cttttaaaac tccttttgga gcagtgattc ctctaattgc tgtatgtgtt
    15961 ggaacatggt tgttattcca agcatctatg catcaattaa ttatgggatt aggagctttg
    16021 gcaattggag taccattata ctttataatg aagtcttata atagaaaaat ctatggtgaa
    16081 aatttaaaga aaatagttta attatgttat aacaatttat tgagttaaaa ttagacattg
    16141 gcagaaaatt aaatacaaat aagtatctac aaggctatat taaaatatta ttaatttttt
    16201 attttaatat agccttatat tgttatcaat aaatgaaaaa tattgtaaca acagaagaac
    16261 aatagtatta tgtttataga caaaaaactt attaggaggt aaatttatgc caacaataac
    16321 attaaaaaat ggtgtagacg tacttattag agaaggtgta agagaagatg ctcaaagcat
    16381 tatagatttt tataatgaag tgggtggaga aactcatttt ctgtcatttg gaaaggatga
    16441 atataaaata tctttggagg aacaagaaaa tgctatagaa tcagcaaaag catctgataa
    16501 ttctgttaaa ctgattgcat ttatagatgg tgaaatagtt gggattgcaa cgattgattc
    16561 taatcaaaaa gcaaaaggaa agcatgttgg agtattagga attgttgtaa aagaaaaata
    16621 ttggggaatt ggattgggta aaaggcttat gttagacctt atagaatggt gtaaatcaaa
    16681 tggaataacc aagaagataa ctcttgtcac taatgaagaa aattataatg ctataggatt
    16741 atacaaaaaa gttggatttg aagtagaaag catactaaag aaagaatgtt attataatgg
    16801 tgtttatact gatttaatag gaatgtcact tttattggga atataattag tgtacatttt
    16861 atctcgaaaa aagtatatta agtatggtat aattaaaaaa gaatataact taatataatt
    16921 ttggaggacg aattatgaaa aatctgtgta aaatatcaga tgattattta aacagcaagc
    16981 ttaagtattt aaagttatta tcaaaacaat atccaagtat atcaaaagca agtacagaaa
    17041 taataaattt agaggctata ttaaatcttc caaagggaac agagcatttt ataacagatg
    17101 tacatggtga atatgaacca tttgtacatg tattaaaaaa tggttctggt gtaataaaga
    17161 gaaaaataga agaattgttt tcaaatacaa taagagacag cgaaaaaaaa atgttagcaa
    17221 ctcttgtata ttatcctgaa caaaagttag acctaataat taagcaagag gaaaatatag
    17281 atgattttta tagaataaat atttatagac ttatagaact ttgtaaatat gcttctagca
    17341 aatatactag gtctaaagtt agaaaactgt taccagaaaa ttttaaatac ataatagaag
    17401 aacttttgca tgagcatgtt aaaagtgaac ataaagaaga gtattataaa agtatagttg
    17461 aaacaatagt tgacatagga atagctaagg agtttataat tgctatttct acagttatac
    17521 aaaaattggt tgtagataga cttcatgtaa taggagatat ttatgataga ggtccaagac
    17581 cagatataat tgtcgataag cttattgaac atcattgtgt agatattcaa tggggaaatc
    17641 atgatatatt gtggatgggt gcagcctcag gtgaaaaaac ttgtatagca aatgcactga
    17701 ggatatcagc tagatatgcg aatttagaca ttgtagaaga catatatgga ataaatctat
    17761 tgcctttagc cacttttgct atagaaatgt ataaagatga cccttgtaaa gaatttattc
    17821 caaaggttaa tgaccaaagt gtaacaacaa cagaaaagtc tttaatggca aaaatgcaca
    17881 aagcaataag tataattcaa tttaagcttg aaggtgaggt tatcagacga agacctgaat
    17941 ttgaaatgga acatagactc ttgcttaata tgataaacta tgatgaaggt acgattaatt
    18001 taaaaggtaa aacatataaa ttaaaagata cttatttacc tacaatagac aaaaaagacc
    18061 catataaatt aactatggaa gaaagaaatg ttattgataa attagtatct tcatttagag
    18121 gtagtgaaaa actacaaaaa catgtttcat ttttgttttc taaaggaagt atatatttaa
    18181 aggctaactc aaatttactg attcatggat gtgtaccgtt aaatgaagat ggaagcttta
    18241 tgtctatgaa tataatggga aaagagtata aaggtaaggc tcttatggat aagatggagt
    18301 ctctagcaag agaaggattt ttctttaaag ataaggcaga agaaaaactg tatggcatgg
    18361 atattatgtg gtatttatgg actgggaaat gttcatcatt atttggtaaa gatgatatga
    18421 ctacatttga aagatacttt atagctgaaa aagagactca taaagaaaat aagaacccat
    18481 attttaaact tagagaaaat gagatggctt gtaaaagatt atttgaagaa tttgatttag
    18541 aacttgatga atctcatata ataaatggtc atgttcctgt ggagagtaaa aatggagaga
    18601 gcccaataaa agctaatggt aaaattctcg ttatagatgg tggtttttct agggcttacc
    18661 aaaaaactac tggtatagct ggatacactt tgatatacaa ttctagaact ttgcaattag
    18721 tatcgcatga accatttaat tctgctgaag aagccattgc aaatgaaagt gatatcttat
    18781 cgacaactgt tgtagtagag cataaggcaa agagaaaaat ggtgagagat actgatgaag
    18841 gaataaagat acaagaggaa atagaagatt taaaactact tttaatggct tataagaaag
    18901 ggctaataaa agaaatgtag atgttataga ggcacttcat gattgaagtg tctttttgtt
    18961 aatacttatt aaatatatta taaagattaa taatagtagt ttatattata cttactaagt
    19021 gaatccaaga taaatgggtt cacttttttt atttataaac attattaaaa aaatatagat
    19081 gttttggtta tattccttgt cctttataaa tagaattata taaacaaagg aggaggacaa
    19141 gagatgaata aactttctga tgaaataata aactctcttt ctacaactat aagagaaaag
    19201 gtagaaaaag tctcaaacaa taatctgaac atagaagaaa ttaggttacg ctcacaaaaa
    19261 ccattgatac taaatgcaaa ctctaaagat tatttttaca atcaaaaaac tatgacactt
    19321 gatttaaatc aacaaaactc atatgtagta acaagagaag atgtagagca aacttttcaa
    19381 ataatatgta aatattcaat acattcattt atggatgaca taaaaaaagg attcataact
    19441 ttgagaggag gtcatagagt agggttagtt ggaaaagcaa tagtagaaga tggacaagtc
    19501 aaaaatataa agcatatatc atctttaaat ataagggtat caagggaaat tattggatgc
    19561 tcggataaga ttttaagtca tatcataaag ggaaaaaatc agattaataa tactttgata
    19621 atatcaccac ctcaatgtgg aaaaacaact ttaataagag atatagtaag aaatttaagt
    19681 aatggtaatg aagattatgg atttaaaggt ttaaaagtgg ctttagtaga tgagcgtaat
    19741 gagatagcag gagcttatct aggtgttcca caaatggatg tgggaataag gactgatata
    19801 atagaaactt gcccaaaaga cttgggtatt acaatgcttt taaggtctat gtcaccaaat
    19861 gttattgtga cagatgaaat tggaagtgaa aaagaaataa aggcattata cacagcatta
    19921 aatggaggta taggattaat aactactgtg catggagatt caatagagga tatacaaaat
    19981 agaaaagaat taaatagact tctggataaa gagttattta aaaaggttat aattctttct
    20041 gcaaaaagag gagcaggaac aattgaaaaa atttatgatt tagaagaaaa gagatggtat
    20101 tttgcaaatt aaaataatta ttatagcttt cttgatagga agtagttatt tgataggtga
    20161 acaaatatac aaaacttata ctcgtagaca taaacaatta aacgatttaa taagagtttt
    20221 agaaattttg aggatggatt tatcttttgg actttataca ttagaagaaa tctttaatag
    20281 gataggtgga aataaagagt tttgtttttg gaaatttttc tatcaaatat cagaaggact
    20341 tcacaatgaa caaagtaaaa ctttggaaat aataatatca gaaaatattg atgtcttaag
    20401 taaagaaact tatctaggca ataaagaaat tgaggaactg aaaaatctaa tacttacttt
    20461 agggaaaagc gatatagagt cacaacaaag aatgatagat ttatctatag aaaatttgaa
    20521 aaaacaaacc tatgagacaa aggaagatat aaacaaaaaa ggcgtattgt ataaaaaatt
    20581 agtgacattc ataggtatag gtatatgtat tatattgatt taatatgggg aggtttatta
    20641 tggaaatatc attgatatta aaggttgcag gtgtcggtat tctaatatca gtattgaata
    20701 tgatattgga aaaaacagat agaaaagatt gggcaggtct tactacatta gcaggtgtaa
    20761 taatcgtatt gggtatggta ataacagaaa taagtgactt gtttaatact gtaagaacaa
    20821 tgtttcaact ttactaaaga ggagggagta atttggaaat aatgcaatta ataggaattg
    20881 caatcatttc aactacactc tgtttagtaa ttaaaaaaga tagaccagag atagcaaatt
    20941 tcatagctat aataactgga gtaataatac tattgtcagt tatgtttaag ctaaatttta
    21001 tagtagatag tatacaagat ttggctaaca aggctaacat cccgactatg tatatatcat
    21061 taataataaa attaattggg atagcatact taatggaatt tgcgatacaa ctttgtaaag
    21121 attgtgggga aggaaacatt gcttcaaaac tagaatttgg aggtaaaata atagttatgt
    21181 caatgtcttt ccctatactt ttgtccattg tagaaatggt ggtgaatata attccataga
    21241 tgtgtatctt ggaatgaata taagtatatt agagagcttt cgttgtattg aggtttatag
    21301 agtgaagaga ggcatggttg cttatgaaaa aaagattttt accaatgatt ataggttttt
    21361 tatttacttt cttttttgta aatatctttg ctattatgat atttgctaat gaagtaccaa
    21421 gtagtgaaga taaagaatat ggtgaaacaa aaaatagcat agataagtat atagatggtc
    21481 aattagacaa gctagatata aatgagattc aagactatat aaataaagaa atagttatta
    21541 atgatgttaa tttaaaatca tttgtaaaag atttaattag tggagaaaaa aatatcttag
    21601 atttattcaa taaagatgga ttgaagatat tgatgtttga tgaatttaaa gcaagtttaa
    21661 aggtagtagc agtaattttg gtactggctc tgttatcatc tattttaaag agtctggaaa
    21721 actctttttc atcaggtgca gtaagtcaaa tagcaacata tataatattt atcacaatgg
    21781 tatcacttac acttattggt tttaaagatg tattacagat ttgctatgat gcaatagacc
    21841 atactgtagg tcttatgcaa gttataatgc caatattaat aacattttta cttttaattg
    21901 gattcccaat aacgtcgact actttgaatc ctatcttcat aggaggagta acatttataa
    21961 atgtattttt taaaaatttt ctatttgtat ctattactgt tgcatttgga attttaataa
    22021 taaacaattt atcaaaaaat ataaggctaa agagattttt ctcctttgta aaacaaataa
    22081 attatgtttc tattggagct atgtttactg tttacttagg tcttgtatcc atacaaggac
    22141 tgtatgtaac cagttttgat aaattcagtg taaagactgc taaatttgct ataggtaatt
    22201 ttattcctgt tgtaggtgga tttgtatctg actctgtgga tattttatta tcttcatctc
    22261 agcttataaa aaatatattt ggaggaatag ggcttatctt acttgttgga atttgcttac
    22321 ttccagtaat aaaaatttta tcagtaatag tagtgtacaa attagcagca ataattgtag
    22381 agcctgttgg agaggatgga atatctaatt ttttaaatga agtggcaaat ttaatgataa
    22441 taatgctagc atcagtaatt gctataacag taatgttctt tgtaactgta gctatcttga
    22501 cctcgataag tgtcgtaagt caagggtaat ttatatatca gtgaaaaata attacattta
    22561 atctaatgtt tttagagagg aggatgctat gttagagggc ataaaggcat ggatagttag
    22621 tgttttgata ggagcattta tagtaaatat agtagatatg attttaccaa gttctaagat
    22681 aaaaccttat gtcaacctag ttttaaactt tatgtttgta tttattgtaa taactccagt
    22741 agtaggattt ttttcaaaag atatgagttt agaagataga atattaaaat ctatgggaaa
    22801 ctacaacaag cagtatgtag atagtacaaa tgctttggca aaagagactg gaaataatag
    22861 tctatccaag ggatatgagg atgggttaaa agaagtactt aaattaaaac ttgatgaata
    22921 tggttatgat ttagaagata tagagcttaa tggggcaaat ataaataata taaaaataaa
    22981 agaaaagaat aatagtgcaa aaagtaataa cagttccaat gaagaaaata aaaataatag
    23041 tactaaaaga gaggaagaaa acataaactc taatgacaaa gaaaattcta aacaagtctt
    23101 caaaaaaggt acagagtatg ggttgaacct aaatgaagaa aaattaaaaa acgacttaat
    23161 aaaggtgctg gatgtttcta tagaagatat acaaattgat aaataggagt tgggagaatg
    23221 ttgaaaaact taaatgacaa agataagaga aaggtatatt cactaataac tatagcagga
    23281 atatgtgtgg tttcattagt attattatct tgttttccta gtagcaaaag cgataaacaa
    23341 gttggaaaaa cagagactaa taaaaatgct gaaaagcaag tcactaaaga acaagaaaaa
    23401 gatgatttag agtcaaaatt aacagcaata ctatctaaaa ttgatggtgc aggagacgtt
    23461 gatgtaatgg ttacatttga atcaagtgaa gaaatacaac ctgcttttaa ttcaaataat
    23521 acaacagaaa caacagagga aaaagatgca caaggtggag aaagaacagt tacgacttct
    23581 agtgaaaata aaacaatgat aacttcaaat tcaagtgacc ctgtagttat aaaaactaca
    23641 gagccaaaga taaagggagt gatagtggta gcaagtggag caagtgaccc aagtgttaaa
    23701 gagacacttt atagtgcagt acaaacatca ttacaagtag ctggtcatca agtagaaata
    23761 tattctaaat aaatacatat aaccgggggg ataaatgaat gaagtttaat tataagggaa
    23821 gaggatttgt aataataact ttaactgcaa tgttagtggt agtaggaaca gtgaattatc
    23881 aactaagtaa gaaatcttta ttagaaacat caaaagagtt taaggcatat gaagaagcac
    23941 agttgcaaaa aaatactgat gagagtgata gtagtaagac agaggattct agtaatatag
    24001 ataaacaaga tggtaaggaa agtgctgata ttgatatagt tgatagcaaa gctagtaaag
    24061 taaaagaaaa ggcaactgag acaagtaagg aaataaaagc tcaattatca tcagagaaaa
    24121 atatgaaaaa ggcatcttat attttagata tgaaaatgaa tagagaaaaa caaagaaatg
    24181 agttagttca agatttaaat gaaatgataa ataatccatc tacaacagaa gaatctagaa
    24241 aagaagcttc taatatgaaa ttaaatatag ttaaaattca agaaaaagaa cttcaaatag
    24301 aaaacctttt gagtacaaaa gggtatgaag aagcgttagt ttatataagc gataacaaag
    24361 ttaatgtagt tgtaaatgaa gctaaattag aaaagaaaga tgcagcaaaa atatttgatt
    24421 tagttgcaga acaagccaat gtgaagtatg aaaatattaa acttacaaat aataatagta
    24481 ataagtaaag tataaacctc agaattaact gaggttattt tcttgcaaag tagacttgta
    24541 tttggtatta ttatacttat aaaaatagtt ccttaaaatc aggaggtaag ttttaatatg
    24601 gaagataata aatttggaca agtaaaaata tctaatgatg taatagctac aatagctggt
    24661 ttagcagcat tagaagttga aggcatagaa actacagcaa cattaacaga taagttgtta
    24721 aaaaataatg gagtaaaaat acaaatcgaa gaagaggatg ttaatttaga tgtaatggtt
    24781 acgataaagt atggaatgtc aataccagat acagctttta aagttcaaga aaatgtaaaa
    24841 aatactgttg agacaatgac aggactgaag gtttctcaag taaatataca cattcaagga
    24901 atcagcttta agaaggataa agttgataaa gaagaagcaa aagcaactaa gaaaaattaa
    24961 tattagccct ctgaaaagtc agagggtttt ttgctggtaa ttgattttat atggataatt
    25021 aaacttaaag gagaattttg attgatgaaa aaagataggg cacaaaagag tacaacaaga
    25081 gaatatataa tgaaacttat ataccaaata aatattaata aagaagattt tgaaacttta
    25141 gaagataaag tagataattt tttaaaagat aattcagaac atattataaa tagatataaa
    25201 gaacttgcac ttcaatactc aaaaaataca aatttaaagt tagaagatac tgaaattgaa
    25261 gatgttatag acaaaaaata tataaataca gtatgcaaag cattgaaaga aaatcatgac
    25321 aaaatagatg agttaataaa taagcatgct aaaaactgga cagtagatag aatgccaaag
    25381 gtagatgttt ctatacttag attgtctgtg tgtgaaatac tttatttaga cactcctaat
    25441 aaagtatcaa taaatgaagc agtagaactt gcaaaaatat attgtgatga taagtctcct
    25501 aaatttataa atggtatatt gggaagtgtt gttgatgaaa ttgggaaata atataataat
    25561 tggaattgat actagctgct atactacttc tatagcagct atctctttag ataagaaagt
    25621 tatattcaat gaaaaaataa tgttagaagt aagggataat tcaaaaggtt taaggcaaag
    25681 tgaagctgta tttcaacata tcaataattt agggattctt agtgatagga taaaatcttt
    25741 taaagataag tttaatgtag aaggagtttg ctcatcaaaa aaaccgagac ctgttgaaaa
    25801 ctcatatatg cctgtattca atgtaggtca taattttgga aaattattgt ctagtatata
    25861 tggttgtagg ttttatgaga ctactcatca agagaatcat attgaagcaa gtttattaaa
    25921 tagtaaatta aaaaataata ataaatttat ttctgttcat atgtctggtg gtactacaga
    25981 gatactttta acaagcaaac aagatagtca tcacaatgta tgtgatacta atttaggtaa
    26041 aattgccaaa ataagtatta aaaaagatga taaaagtaag ttatacaata attttggata
    26101 taatatagat ataattggag gaagtaaaga tattagcttt ggtcaactta tagatagagt
    26161 aggaattaag cttggatata aatttccttc aggaaagtat ttggatgaaa atgctttaaa
    26221 ttgtaatctt aaaatagaaa gtggtctgaa aacttctgta agagatgggt atatgaattt
    26281 atcaggatta gaaaatcagg taaataagat tataaatgat aatggatata atactaatca
    26341 aaaagaatat atatctaaat tggtattaga ttcagtagtt agaaatatgt ttaaatcttt
    26401 agtgtattta tgtgaaactt ataatgtaaa tgaagtagta tttgctggtg gagtttctgc
    26461 aagtaagtat attcttagag aattaagcgt gaagttaaga aaaaagcata tagaagctta
    26521 ttttacagaa ccacaatatt caactgataa tgctgtcggg tgtgctataa taggattgaa
    26581 taacttttta ggagaaagag tatgaaacta agagctttag atataagtga agcaaactca
    26641 tatattaaaa gaattttaat aaacgaccca atactttcta atcttaaagt taaaggagaa
    26701 atttctaatt ttaaggttca tagtagtggg aatgtatatt tatcacttaa agatgaaaca
    26761 tcaaagttaa actgtgtaat ttttaaaagt aattttaatc gcaatttaaa attagataat
    26821 ggtgtaaaaa ttatagctaa tggatatata tcagtctatg aaagagatgg tgcgtatcaa
    26881 ttatacatta atgaaattga aatagaggga ataggaaatt tgcacataga gtttaataga
    26941 ttaaaggaaa aacttaataa agaaggatta tttgacccta aatacaagat acctatacct
    27001 aaaatgccaa actcaattgg cgttataaca tctccaacag gtgctgttat aagagatata
    27061 ataaatgtaa taaaaagaag atatccgaaa gttaatataa agctttatcc agtaacggtt
    27121 caaggagata aatcagctga agaaatctgt gaagcaatta gatttttcaa tcatatgaag
    27181 aatgttgata cacttatagt tggtagaggt gggggttcaa tagaagaact ttggtctttt
    27241 aatgatgaaa tggtagcaag agaggttttt aattctcaaa ttccgataat atcagcagtt
    27301 gggcatgaaa cggattttac tatttgtgac tttgtgtctg atatgagagc cccaacgcca
    27361 tcagcagcag ctgagattgc tactccatca ttggatgata taaactataa acttggaaac
    27421 ataaaaagta ggatgagtaa gtctcttaca aatcaaatag agctagacca atataggtta
    27481 gaaacagtat ttaataagat aaataactat ttagattcgt acacaataaa agataaagtt
    27541 atacaattag acaaaatata tgataaaata atttttggaa tagaaaataa tttaaaatta
    27601 gaagatgaaa aattagtaaa aattggagca ttactccata atttaagtcc attagctact
    27661 atggatagag gttatagtat aactcaaaaa aatggaaaag taataaatag tataaaagga
    27721 ttaaaaataa aggatagtat agatatagta ttaaaagatg gaaatttaga atgtatgata
    27781 gataaaattg aaaataaaga gggatagtat gaatttaacc tacgaagaag cttataaaag
    27841 attagaaagt attttaagtg aattagaatc taaaaatgct agcttggatg aatctttaag
    27901 cctatatgaa gaaggtataa gcctttataa acattgtaat aagcttttag atgatgctaa
    27961 attgaaaata agtaaattta atcaattggg aatagaagaa gactttaaaa tagaggagga
    28021 ataaaattgg aatttaaaca atgtctaaaa gaaaaagcaa gttttgtaga aaaagtactt
    28081 aaagaatata tgcctaaaga agaagggtat caaaaaactg ttattgaagc tatgaactat
    28141 agcttaagtg ctggaggtaa aagattaaga ccaatactta cattggaggc ttgtaaaata
    28201 gttggaggta atgaagatga agctattcct tttgctattg caattgaaat gatacataca
    28261 tattcactaa tacatgatga tttgccagct cttgataatg atgatttaag aagaggaaga
    28321 ccaacaaatc ataaagtata tggagaagct atggggattt tagcaggaga tgctttgtta
    28381 aactatgctt ttgaagtaat gcttgcaggc tcaatcaata aagaaaatcc agaaaagtat
    28441 ctaaaagcta taaatgaaat tgccaaaggt gctggtatat atggaatgat aggtggtcaa
    28501 gtagtagatg tagaaagcga aaataaacaa attgaaaaag aaaaattaga ttatatacat
    28561 atgaataaaa ctgctgcaat gatggtaggc tgtatgagag caggagctac tataggtgga
    28621 gcaaattctg aacaaatgga agaaataact aaatatgcaa aaaatatcgg attatcattc
    28681 cagatagtag atgatatact agacatagta ggagatgaag ctaagttagg taaaaaggtt
    28741 ggaagtgata tagaaaatca taagtcaaca tacccatctc ttttaggttt agataaatct
    28801 aaagaaattg cacataattt gattgatgaa gcaaagaaaa gtatagaaaa attatctgat
    28861 gatgtagatt ttttaaaggg attggctgaa tatataatag atagagaata ctaaaatata
    28921 cagctaaaat cgaggaggga aaaatggatt ttttttcgga gatttttaac aatggggctt
    28981 tgggaataag tctgattgct tgtttcttag cacaatttat aaaaatattt actggtaagg
    29041 aaaaaagaat tgagttatca agaatactta tttcaggagg tatgcctagt tcccatagtt
    29101 cttttgttac aagtttagct acagtggtag gtattgaaaa agggtttaat tctactgatt
    29161 ttgctataat taccgtgctt gccttaatta taatgtatga tgcagcaggt gttagacggg
    29221 ctgttggtaa acaagctact atattaaatc aaatggtggc agatatacag catggaaagc
    29281 atatagagca gaagaaatta aaagaattaa taggtcatac accactagaa gtttggtttg
    29341 gagctttact tggaatagta acagctttaa tattgatgta aattgaaaat ttacagaata
    29401 aaggtgagga taatatgtat aaatatttag ataaagtaaa ttctccaaag gatataaaaa
    29461 atatgagtat agaagaaatg gatttacttg caaaggatat aagaaagttc ttagtaaaat
    29521 ctgtttcaaa gacaggagga cacttagctt ctaatctagg agtggttgag ttaacattag
    29581 cacttcataa agtatttgat agccctaaag ataagattgt atgggatgta gggcatcaat
    29641 cttatgttca taaaatagtt actggaagaa aagattgttt tgtatctttg agacagttta
    29701 atggactaag tggttttcca aaggagaatg aaagtcctca tgatattttt gatacaggac
    29761 atagcagtac atctatttct atagctacag gtattgcctg tgctagggat ataaaaaaag
    29821 aaaactatag tgttatatct gtgataggag atggttctat aacaggaggt atggcacttg
    29881 aagctttaaa tcaattagga tatatagata ccaatatgat agtaatcctt aatgataatg
    29941 agatgtccat agataaaaat gttggaggaa tgtctaaata tctgtcaagt attataagaa
    30001 actcaacagt tgagaaaatg acagatgaag ttgataaaat tttaaatgtt acacaaacag
    30061 gtgaaatttt atctaagact gcacatagat ttaaagataa actaatgtat agcttttctc
    30121 ctcaggactg ttcatttttt gattcattag gtataagata ttatggacct atagatggcc
    30181 ataatactaa agaactaata gatatattga gaaaagctaa acacaaaaaa gggcctgttc
    30241 ttttacatgt aattacgaaa aaaggaaaag ggtacagatt tgcagaagaa caacctgata
    30301 aatatcatgg agtatcaaag tttgatataa agacaggtgt tacatctgca aaagttaagt
    30361 ctatgtcaat tagtgttgga gaaaaacttg ttgatatggc taatagtaat gaaaatatag
    30421 tagcaataac agctgcaatg ccatctggta cagggttaaa cctgtttgaa agtgcatatc
    30481 caaaaagata ttatgatgtt ggtatagctg aacaacatgc aacaggattt gcagcaggtc
    30541 ttgcaaaaaa tggtatgaaa ccttactttg ctgtgtactc atcatttttg caaagggcat
    30601 atgaccaagt tattcatgat gtatgcataa caaaaaagcc agttactttt cttatagata
    30661 gagctggatt agttggaaat gatggagaaa ctcatcatgg tatgtttgat ttaagttact
    30721 taaattctat tccaaatatt gtagtaatgg ctccaaaaga cacaagagag atggagctta
    30781 tgatggattt gtcattaaaa ttagattgtc cactagcaat tagataccca agaggaagta
    30841 gttattacct agataaggga gagtatggag agattgtact gggtaaatat gaggtattag
    30901 atgacggtca agatacagtc atactttgta ttggaagtat ggtaaaacat gctttagagg
    30961 ctaaagaaat attatcaaga gaaggaataa atccgacaat agttaatgct agattcttaa
    31021 agccaattga tgaaggtatg ttaaaagcat tattaaaaaa tcataaaaat gtagttacta
    31081 tagaggataa tattgtaact ggaggatttg gaagtagaat aaataagttt attatagata
    31141 atgaatataa cgtaaatata cttaatatag caataccaga agaatttgta aaacatggca
    31201 atatagatga attatatgat tttgtaggat tatcgccaaa aagtattgct gataaaataa
    31261 ggaaattagt tattgagtaa aaatctaatg tagatattgt aaattaaaat ttatataaaa
    31321 tatagaattt atcaattaga ataaatatca agtgtgttac ttcaagctac tttagttatc
    31381 attaaagtag cttttaacag aaaggtaatt gtatgaaaaa aagaatagat ttattattag
    31441 tagaacaagg gtactttgaa agtagagaaa gagccaaaaa agcaataatg gctggattgg
    31501 tttttgttga taatcaaagg tgtgataagg ctggtacaga agtaaaagaa gattgtagta
    31561 tagaagtaaa aggaaatcca ataccatatg tcagtagagg tggtttaaag cttgaaaaag
    31621 caatgaaaaa ctttgatttg actattgatg gaaaggtatg tatggatata ggggcatcca
    31681 caggtggttt tacagattgt atgctcaaaa atggagcaat aaaagtattt tctatagatg
    31741 ttggttatgg tcaacttgca tggaaattga gacaagatga tagggttgtg tgtatggaaa
    31801 gaaccaatat aagaaatgtg acaatagaag atacaaagca gtttgctgat tttgcgtcaa
    31861 ttgatgtgtc ttttatatcg ttaaagttag tacttccaaa ggcaaaggag ttagtacgtc
    31921 atgatgggga agttgtcgct ttaattaaac ctcaatttga agcaggaaga gaaaaagtag
    31981 gtaaaaaagg agtagttaga gaaaaatcta cacatataga agttataaaa atgatttctg
    32041 atttctcagt cgaaaatgga tttgagattt tagggttaga tttttctcct attaaaggtc
    32101 ctgaaggtaa tatagaatat ctaattcatt tgagaaatgg aaatgaagga tatgaatttg
    32161 atggtgagac atacaataac aaaatagtag aagttgttga agcttctcat aatttagaca
    32221 aataggttaa aagggggctt agatttgatc ctcgaattat atatgaaaaa ttgtgcctta
    32281 gttgaagaac taaggttgaa tattgataaa aacttaaata tacttacagg tgaaactggt
    32341 tcaggaaaat ctataataat agatgcactt ggactttgtt taggtgataa atatgatagg
    32401 tcttttttaa gaaaaggaac agataaggga cttgttgagg ctgtattttt ttcagacaat
    32461 agatatctta aaaaaatatt agaagaaaat gatataagta tggaagatga taatttattg
    32521 gtaataacta ggttgatata ctcagatggt aagagtactg ctagagtaaa tggaaggact
    32581 gttaaagtgt ctcttcttaa agaaattgca tctacactta tagatataca tggtcaacat
    32641 caaaatcaag ctttgtttaa taaagatact catttaaaat ttctagacct ttttggagaa
    32701 aatgaattgg aagagtttaa aattgcttat aaaaatgtgt accataagta tagtgaagta
    32761 aaaaaagctt taaattgctt gacagaaaat aaagatgaga tgcaaattca acgagaaata
    32821 gatttattaa gatttcaaat aaatgaaatt gaagctgcaa atctaaataa gaatgaatat
    32881 gaagatttat taaaacaaag agaagtttat agaaatagtg aaaagatata taataatcta
    32941 aattcgagtt atagcaagtt acataatggt gagtataatg ttattgacct aataggtctt
    33001 gcatctaagg aacttaatga catatctaaa tatgacagtg ttttaagtga atatagtgat
    33061 actgtagaaa gaatcatgta tgagttacaa gacatatctg gtgagataag aaattacaaa
    33121 gataatatag actttgaacc ttatgagcta gagcagattg agcttagaat cgacgagatt
    33181 aataacttaa gaagaaaata tggagacagt atagatgata tatttgagta ttatggaaaa
    33241 attaaagata gattagatga aatattaaac agagatgaaa gagttgaaca gttaagaagc
    33301 aaactgatga atatagaaga agatttaaaa ataaaagcca gtaaattgac taaagcaaga
    33361 atagaagtag caactacttt agaaaaaata cttcttgatg aattaaaaag cttaaatatg
    33421 aaaaatgtaa tgttcaaggt taattttgaa aaaggtccat ttactttaga tggagtagat
    33481 gatattgaat ttatgatatc ttttaactta ggagaagata ttaagcctat atataaagtt
    33541 gcatcaggtg gtgagatgtc cagatttatg ttagcgttta aaaccatatt ggctgatata
    33601 gatgatattg atacattagt gtttgacgag atagatactg gaataagtgg gatagcagca
    33661 cagatagttg gtgagaaatt gagtgatata gcaaagaaaa aacagataat atgtataact
    33721 catttacctc aaattgctgc aaatgcagac actcattatt gtatagagaa agatacttca
    33781 aataatagga cgtttaccaa tgttagtaag ttaaatgaat ctcaacgaaa aaatgaaatt
    33841 gccagactta tagcaggtaa caatataaca gaaaaaacta ttgaacatgc aagtgaaatt
    33901 attgagttgg ctaaaaaatg ttagtgaaaa acaagcattt taataggtgt aaagaatttt
    33961 tatagaaatg ctttacatct attttttatg atgaaattat tttaaaatat ctagtgccta
    34021 tattgaagaa aagtagattt aaaaatagaa gaaaatttgt ataaatttgt aactttgaaa
    34081 tttctaaatg cttaagtata atattattta ttaaggtagt agtgaggggg acataaaatg
    34141 aataaaaaag atactatgca cttaattttt acaattgtaa tttttatttt attatggtat
    34201 attgtggtag cagtaagtga gattaataaa aatattgaaa gttgtagtaa taaattagat
    34261 tatttgtcac aagagattat gagtactaaa tcggatatta gtaatactat aaatgaagaa
    34321 atgtcaaaaa gttatataac aaagagtatt gacttaaaag taaaaaaaat cgagaaaaaa
    34381 gagtgtgtta ttgatgtaga tgttcaatta agtaaacttg gaaaaaatgg aaaagtattt
    34441 tttatgtata aagaagatag tgataaatgg aatgaaactg agatgacaaa acatggagaa
    34501 ttatcatatg catgtgaaat taagataaat acaggaagtg aatatgacta taaagtagtt
    34561 acaagtggag ctatatctga aagttctgat gtgcaaaaat tgactaaatc tgattatatg
    34621 cctgaacaac ccatatttaa ttctgggata agagataata gagaatactt tattgaaata
    34681 gtagaaaata gtaatctttt caatcatgag agtgaaaagt caaaaaataa agtttatgat
    34741 aatattagat tagaaaaagt agatattatt gtaaatgaag gaaaaaaaga cactatatac
    34801 aaagccaaat tagctgaagg cttagatgat ggcaaaacta ataattcaaa tagaaatcaa
    34861 gtgaattatg aagtaagttt tcctaaaagg gatattgatg acataattta tattaaagct
    34921 aaacttacat acaataatgg agtagaatat acaaaggata taactgaaga tataactatg
    34981 ggattacaag atttagaaaa ataaatacat atttaaataa atattcaaat agatttgttc
    35041 aataaaatag agtataaata aaactgtagt agttttactt atactctatt aaaaaattat
    35101 aattttaatt ccatatatct agccccttca attggattat aagtataagg ttctatatca
    35161 taaaaaccaa acttttcata taatccttgt gcacttttca tgcttggaag agtatctaat
    35221 ctcatgtgtg tatatccaaa ttttttagct tcttcaatga tttcttccaa tagtattttt
    35281 cctattttta atcctctaaa ttgatttctt acatataatc tttttaattc acatatttta
    35341 ccttcaagtt tttttaaagc aacacaacct gccaaatttt catcaacaaa tgccaatatc
    35401 aatgagcctg aaggtttttt atattttcct ggaagtgttt ttaattcatt attaaagtct
    35461 tgaaaacata agtctatatt tagagaattt gaatattcag taaatagaag ttttacgttt
    35521 tcaaggtttt ctaaatcttt ttcttcaaca aattgtaaag ttatattttg agccatttta
    35581 tccgccatta tataaacatg tttatataat gacttcacct ccttgatata tgtatatttt
    35641 tcttaatatc aattagattt taataccatt actttttcac caccatagtc aagctctcca
    35701 ttaaactcaa atccaaaatc tttataaagc ttaatagcca ttttattgtc ttcgaatata
    35761 ctaagatata gttcatcaca gttatattca tttttaagat gatttacaag aaaatctaat
    35821 gatgcttttc catatccttt accttgatat tttgaatcaa tcataaatct atcaagccaa
    35881 actctatcat cttctaaacg accatacata gcaaatccta ttaaagaatt ttcatcatat
    35941 attccaacag gtacccaagc tgttataaat ttagattcag caatagaaag tgcattgctt
    36001 tctatataat ctaactgact tttatctaca gacaaactaa ctaaatcatg ccaattattt
    36061 atgtctgcaa ttcttacatt taatttcaag atattaccgc ccttcaaact ttttgttaca
    36121 atctaatact atcatattat gtattaaaaa tcacttatta agattttaaa taaacttttt
    36181 aaatgaataa attctcagga aaaaggaatt ataaatacat aataatacag cataggagta
    36241 gatggatata tgaacaaaat aaataaaaat ttcaaaaata tttggataat attaggagct
    36301 gtatcgattt ttaccatggt tataattttt aataaaaatt gtaatgttaa taaagagact
    36361 caaatagaag cttttacaat taagaatcat gataaaaaat ttgtatatcc tatgggaaat
    36421 ataattggag taaaagcaaa tacagatggt gtattggtct taggatatga ggaagaggat
    36481 gtagactata ttggaggcat acaaatcggt gataatatag taaaaataga taataaaagg
    36541 ataaaaaata gtcaagatgt atcagagatt ttgaataaga ttaaaaaaag taaagttgaa
    36601 gtgacttttg agagaaaaaa taagtataaa acagaagtaa tagagactaa aaaagaaaat
    36661 ggcaagtata aacttggatt atgggtaaga gataaaatat ctggaattgg aactatgact
    36721 ttttacgacc ctagtatgga aaagttcaaa gcaatcggtc atgccataaa agattcggat
    36781 actaatgaac tattaaagat aaagcaagga tatatatata agccagaaca attaaaaata
    36841 gtaaaagcta gtaatgaaaa agtaggtaaa ataaaaggag attttaatga cagcaattta
    36901 atgggtaatt tctcaaataa ttcagagtta ggtataagtg gtaatattac agaaaaccat
    36961 aataaagagt ttaatgtagc aaataaagaa aagcaattaa tagaagttgg aagaccgcaa
    37021 gatgtaaaaa taggagatgc agtaattctt tttgaggata aaaacaaaaa tataacaagc
    37081 tatgatataa aaatagaaag tatagtatat gataaaggaa attatagaga tatggtaata
    37141 aaagtagtag atgacaagtt attggaatac acaggaggta tcgtacaggg gatgagtgga
    37201 gctccaataa tacaaaataa taaaattatt ggtgcaataa ctcatgtttt tagagataat
    37261 ccgaaaaaag gatatggtat ttttatagat gaaatgataa aattgtaggt gaggcattaa
    37321 aaaattttat tattttatca attatctagg aggaatataa ttttggagtg tcgaatatgc
    37381 tttagagtag ataattagga agcaattgtg taaaaagttt agttttctgt aataagaaga
    37441 tgttttttaa tggggggatt tttagtggaa aaaatcaaaa tagttttagc agatgacaat
    37501 aaggattttt gtcaggtatt aaaagagtat ttgtctaatg aagatgatat cgatatatta
    37561 ggcatagcta aggatggaat tgaagcatta gacttagtaa aaaagacaca accggactta
    37621 ttaatactag atgtaataat gccacatcta gatggattag gtgtaattga aaaattgaat
    37681 actatggata tacctaaaat gccaaaaata atagtactat cagcagtagg tcaagataag
    37741 ataactcaaa gcgcaataaa tctaggagca gattactaca tagtaaagcc atttgatttt
    37801 gttgtgttta taaacagaat tagagagcta gtttctaata gagttacaca agttgagcca
    37861 aaacctagac cagttcaaga gactcaaatg actagaagtg attttgttaa gaatgtagga
    37921 aatatagaga cagaaatcac aaatataata catgaaatag gagtaccagc tcatataaaa
    37981 ggatatttat atttaagaga agcaataaaa atggttattg ataatgttga gcttttaggt
    38041 gcagtaacaa aagagttata tccaagtata gctaaaaaat tcaatacaac accaagtaga
    38101 gttgaaagag caataagaca tgcaatagag gttgcatgga gtagaggaaa agttgacaca
    38161 ataaatcaat tatttggata tacggtacac aatactaaag gaaaaccaac taattcagaa
    38221 tttatagcaa tgattgctga taaattaaga ctagaacata gtatggttaa ataaacaaga
    38281 cataaaaagt aaggcttttt aattaaggca ttggctataa atgcgtatta caagcagcga
    38341 aacggtataa ccactagggt tataccgttt cgctatttta ataaatataa aaattttctt
    38401 tattatttgc ttactatatc aatataataa ttttattata ctatggatat agtatgtgtc
    38461 tttacaagtt gtaaactgac agtggtttat tttttaatat aaatattgac tttgatgcag
    38521 gtaaactttg tatttttaag cgtattatgg aatatgttaa ataaaaaaat gatgaaatat
    38581 agtattgtaa atgccaaaga tgcaaaacaa acttaaaaca tttattttat tgttaagtaa
    38641 tgctataata taatgtgatt ttaataatga tagtggaggt ttaaatatga gagtcgaggc
    38701 ccctataaaa gtagatcgaa aaaccaaaaa acttgctaaa agagttgaaa gtggggaaat
    38761 agcagttata aatcatatag acatagatga agttgctgca aactctttag tagaagctaa
    38821 aataaaactt gtcataaatg cggctccttc tataagtggt aggtatccca ataaaggtcc
    38881 agggatatta actgaaaata atatactaat aattgataat gttggagaag agttatttga
    38941 agaattaaaa gaaggagaaa ctatagaagt tgtagatgga aagatatata gaaaaggaaa
    39001 atttttaggt gcaggagaag ttttagacaa gtatgaggtt tcacaccaaa taaaggctgc
    39061 ctatgagaat ttagctgtag aattggatag atttatagat aatactatag attatgctaa
    39121 aaaagaaaaa ggatttatac ttggagaagt agaaattcca aaagtgaaaa ctaattatgc
    39181 taataaacat gtattgatag ttgtaagagg tcaggactac aaagaagatt taagtactat
    39241 gctttcttat atagaagagg taaagccagt attggttggt gttgatggag gagcagacgc
    39301 tttaattgag tttggatata ctccagatgt gattgtaggt gatatggata gtgtaagtga
    39361 tgaagcttta aaaaaggcta gtgaaatagt agtacatgct tatacagatg gaagagcacc
    39421 aggtcttaaa agggttgagg aattaggctt agatgcagtt gtatttcctg caccaggaac
    39481 tagtgaagat atagctatgc ttatcgctta tgagtataag gctgagttaa tagtagcagt
    39541 tggtacacat tcaaatatga ttgattttct tgaaaaagga agaaaaggta tggcaagtac
    39601 ttttttagtt agactaaaaa taggttcaaa gcttatagat gctaaaggcg ttaatttgtt
    39661 atacagaagt aaattaaaaa taaagtacat atgggcatta atagcaacag cattatttcc
    39721 tgtgttggta gttgcatcat tgtcaccagg agttcaacag ttcatacaac taatgcaatt
    39781 aaagtttaga gtattattac agatgtaaat tttaggagga taaatatgca tattaatatg
    39841 aaatattata tagtcactat aggggctata tttatagctt tgggtatagg catgttagta
    39901 ggatttaatt taaataataa tcaacaatta agtgaacaac aagcaaatat aataaatgat
    39961 ttggatgata agtttgatat cttaaaagaa aaaaatgata aacttgacag tgacttagca
    40021 agtgtaaata gagattatga agaggcagtt aattttataa ataaaaatgt agataagatt
    40081 ttagctggtt cgctgaatgg taagagtata ggaataatat ctgctaatga aaatgacgat
    40141 tatacaaaaa atatagaaga cataataaat aaatctaatg gaagcattgc atttaatata
    40201 atattaaaag aaaacataac aaatccagaa aagttaaagg aaatgtcaac taaactaggt
    40261 acagatgtaa aaaatgcaaa tgatgcagtt aattatataa ttgacactct taaaaaggaa
    40321 gatgcaagtg acattttaac atatcttcaa gaactagatg taataaagtt taattttatt
    40381 ggagatacat atctaaaata tgattccgtt gtaatagctg gtggaaatga tgcaaaagac
    40441 agtgcaaaac agtttgaaaa aatagaaaaa tttgtagttt caaaacttaa atcagagaat
    40501 aaatatttag tagaagttca aaatacagga gttaaaactt cttatgtaga actatattct
    40561 aaaaacaaag ttgcaactat agacaatatt gatgaaggta taggaagtat atctctagcg
    40621 attcttctac aacaaggtaa tatagttggt aattttggta gattggatac tgcaacttca
    40681 ttgttgccat ctattaaata gaaataattt taaggagaga aaaaatggat tcttatataa
    40741 gtataataat accagcttat aatgaagaaa gtagaataaa atatacatta gaaagcatat
    40801 tggacattaa gcaaataaat gaaataatag ttgtggatga tggttcaatt gacaatactg
    40861 ctaaggttgt atctgagcta gaaaatgaaa agattaaatt ttttaagtta gataaaaata
    40921 gaggtaaagg atatgcttta aattatggta tcaaattagc aatgaaaaat gcagatatta
    40981 taggttttct ggatggagat ttaggaagtt ctgctaaaga agttgaaaaa ttaatagtac
    41041 ctatattaaa tggtgaggcg gatgttacaa tagccaagtt tcctcctgca aagagaaaag
    41101 gaggtttggg atttgtaaaa ggtttagcta aaaattctgt gtttgagatg actggtgttg
    41161 agttagatgc aactttgtca ggtcaaagaa tatttaaaag agaagtttta gaaaagtttg
    41221 atgaaatacc ttttggatat ggagtagaag ttggaatgac cattgatata ttaaagtatg
    41281 gatttaaaat taaagaagta ttagtaaata tgactcataa tgaaacagga agaaatttaa
    41341 aaggttttat tcatagaggg aaacagtatt atcacataaa aaaggtttta ggacaaaaaa
    41401 agaaagaatg gaggtaatat cttagtgaaa gattatatac tttataccat tttattatta
    41461 actggattat ttggaaccta tgctgtaatt ccactattta aaaatttatt gataaatgga
    41521 aatgtactta gaccaaacta taaaaaagat atgataccag taagtatggg aattgtattt
    41581 ttgcctatga ttgtaatcaa tggaataata cttgctttct ttactacaga atttaaggat
    41641 ttagcgtata tatttatgtt tatgtttggt atgatatcca tgttttttgc agggatacta
    41701 gatgatgtca ttggaaatag agatgtaagt ggtttaaagg ggcattttaa aagtttattt
    41761 aaaggaagtc ttacaacagg aggttttaaa gctttatttg gtggatttgt agggcttata
    41821 gtttctgttg ctatatcaaa agacataatt gatataatag taaatacact aataatagct
    41881 ctatcaacta atttaatgaa tcttttggat ttaagaccag gaagggcaat aaaagcatat
    41941 ttattcataa tggtaattat atttttaaca ttgacaggat ttgttcaagt tcttccatta
    42001 cttatagtac caaatgtact tgcatacttt aattatgatt taaaagcaag ggctatgatg
    42061 ggagatactg gttcaaacgt tcttggaata tctattggaa tgctcatttc ttttgggtat
    42121 ccatttaaca ttagattagg atggttaata tttgtagtta ttatgcatat attcactgag
    42181 aaatactcat taactaagat aatagaaaaa aataaactat taaattttat agataaattg
    42241 ggaaggtagt attatgataa gcaaaagagt ggggattgtt gaatcaatag taagtcaaac
    42301 tgaaactcta gatgatataa gagtaaatat aaatggagaa atacaaaagg catataatta
    42361 tccaaaaata tctggaacta taaatatagg tgacgaagtg gttttaaata caacagcagt
    42421 agaactgagc ttaggtacag gtggatacca ttttgtaata actaatttaa ataatataga
    42481 atctacttta actgaaggtg gacatataat gaaacttaga tatacgcctc ttcaaataaa
    42541 agtggattct gttgaggaac aagaaagtat ttatcatgat aaatttgcga attttaaagg
    42601 attagaaggt ctttctgttg tagtagggac tcttcatagt atgttaactc catttgcagc
    42661 ttcatttaaa agaaataatc caaataaaaa attagtgtat ataatgactg acggagcatc
    42721 tcttccaatt tatttaagta aaaatgtgga tactttaaaa gaaaaaaaat taatagatag
    42781 tacaataact ataggaaatg cgtttggtgg agattatgaa tgtataaata tatatactgc
    42841 attaattaca gcaaaagaaa tactaaaagc ggatgcagta tttgtcagta tgggacctgg
    42901 aattgctggg acaggaacaa aatatggatt tacagggatt gaacaaggct ctattcttga
    42961 tgccgtaaaa aaacttgaag gaagagcaat tgcaattcca agaattagtt ttgctgacaa
    43021 acgagaaaga catcaaggta tttcacatca tagtatgact gtatttaaag aaattgtaaa
    43081 tgtaaatgta gacataccaa taactatata tgatgatgaa aaattgaact ttataaaaga
    43141 gcaaatcaat ttaaatggtt tagacaaaaa gcataatata atttatatag ataataataa
    43201 aacgaaagag gatttagact attttaatct aaaagtaaag agtatgggaa gaaattatga
    43261 acaagatgag gcatttttca aagctgcaag cacagcagca tattacttaa tggaggttta
    43321 agatggtatt agaagaaaaa actattagta gtgatagggt ttatacaggt aaagtaatta
    43381 ctttaaaggt tgatactgtt gaaattccag ggcaaggata tcaaaaaagg gagttagtag
    43441 aagttggtgg agcagtagga atagttgcaa tcacagatga taataaagtt gtgttagtta
    43501 agcaatttag aaaacctata gaaaagccaa tctttgaaat tccagctggt aaactagaaa
    43561 aaaatgaaag tccaaaagaa tgtgctgaaa gagaattaaa agaagaaact gggtatagtg
    43621 caaaaaatat taagctaata cataaatttt tcacttcagc aggtttttct aatgagataa
    43681 tgtttgtata tttagcaaca ggtcttactc caggagagaa taatttagat gcagatgagt
    43741 ttttagatgt ctatgaaatt gagcttgaag aagcatataa catggtatta aaaaatgacg
    43801 ttgaagatgc aaaaacatct attggattat tattagtaaa agatatgttt aaaaattaat
    43861 atttataaaa tgaataaccc tttgtcctaa agcatattat tttataaagg acaaggggga
    43921 attaaattga gaagaacata tagaaaaaat gattttagag aattaaatag acaaataatt
    43981 attataggat tgttgtttat gatgtctatt gttgttgggt cttacataaa taaaatattg
    44041 ccaggttcta gtaacaatat attaaacaat ataaatcctg ctgtagagta ttataatcta
    44101 aatataagca taaaagatac agtgatacaa aatttaaaat cagatgcaat ctttatgggc
    44161 tcaatagctc tattaagtct atttgtagta acaattccag cagtgttggt agcttttgta
    44221 ttaaaaggga tgtcaattgg ttatacaata aatagttgta tattggcatt aaaatttaaa
    44281 agtataaaaa tgattttaat aatattattt aaaaatttga ttattattcc atgtgcaatc
    44341 a
//
LOCUS       DAELLV010000008        43282 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.008, whole genome shotgun sequence.
ACCESSION   DAELLV010000008 DAELLV010000000
VERSION     DAELLV010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 43282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43282
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.008"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(121..894)
                     /locus_tag="KPD58_000393"
     CDS             complement(121..894)
                     /locus_tag="KPD58_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HBG0264184.1"
                     /translation="MKNVANATHYLNMDTKELFNLYNKNKNVDIRNILIERHLYLARL
                     LAKKYINKGVDFEDIYQVASLALIYAIDRYDVEKGFEFSSFATPTIVGEIKKYFRDKV
                     WTLRVPRRIQELSKKISDAKIKLEQENKKHPKVKDIADYIGVSEEDVLEAMEASYGYQ
                     PMSLDSSSNDDSEDKDITLIDKIGKEEGNFGSIEYEDFINKFIETLNELEVKIFKDRF
                     FFDKTQSSIAKELGISQMTVSRLEKKVVEKLKKEYEKNL"
     gene            complement(894..1280)
                     /locus_tag="KPD58_000394"
     CDS             complement(894..1280)
                     /locus_tag="KPD58_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0264185.1"
                     /translation="MEITTNPDYVSIIRLTASGIANKMGFPIDDIEDIKVAVSEACTN
                     AIKHSKDDVFNIVFNILDNAINIEIQDNGKGYDVSSISTPDLENPKESGLGLFIIKTL
                     MDDVDIESEHNQGTKIKMTKYLGVDI"
     gene            complement(1305..1622)
                     /locus_tag="KPD58_000395"
     CDS             complement(1305..1622)
                     /locus_tag="KPD58_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HBG0264186.1"
                     /translation="MSMNIDSNLDSQNKFWNVCLDGELDVSTADKLKEHLHALIEKNM
                     LDVKINLKDLDYIDSTGLGAMIGVLKKLKINEKEIYIVNPKSNVRKIFTITGLDKIFK
                     VEG"
     gene            complement(1634..1894)
                     /locus_tag="KPD58_000396"
     CDS             complement(1634..1894)
                     /locus_tag="KPD58_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HBG0264187.1"
                     /translation="MNAWGWQIGAVLVGSSALIVAIYLAKTLNSINKVVEKAYKIVDY
                     NERHIQDIVENASSISKGIDDIVYVINKILSIGNIFKIIKRK"
     gene            complement(1894..2190)
                     /locus_tag="KPD58_000397"
     CDS             complement(1894..2190)
                     /locus_tag="KPD58_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBG0264188.1"
                     /translation="MCNKRSCKKGGFLLLGAILGFIFGMFFAPKKGSELRKETKEKFN
                     DVKENPKEVLHETFNDVKERIINLVDDDNNEEDIKISEEDIVISKSFDDEGDVN"
     gene            complement(2259..4685)
                     /gene="gyrA"
                     /locus_tag="KPD58_000398"
     CDS             complement(2259..4685)
                     /gene="gyrA"
                     /locus_tag="KPD58_000398"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HBG0264189.1"
                     /translation="MEENNKILPIEIAEEMKKSYIDYSMSVIAGRALPDVRDGLKPVH
                     RRILYSMSELNLTPDKPYRKSARIVGDVLGKYHPHGDTAVYYAMVRMAQDFSTRALLV
                     DGHGNFGSVDGDSPAAMRYTEAKMSKLSLELLRDIEKETVDFKPNFDESLKEPSVLPA
                     RYPNLLVNGSNGIAVGMATSIPPHNLAEVIDATVYLIDNPECSVDDLIKFVQGPDFPT
                     AAIIMGKESIAEAYRTGRGKVKVRSRAFIEELPKGKQQIIVTEIPYQVNKAKLVERIA
                     ELVKEKRIEGISDLRDESNRNGMRIVIELKRDANANIVLNNLYKHSQMEDTFSIIMLA
                     LVDGQPRVLNLKQILYHYIKHQEDVVTRRTKFELNKAEARAHILEGLKIALDNIDAVI
                     SLIRASKTGQEAKLGLIEKFKLTEIQAQAILDMRLQRLTGLERDKIEAEYEDLIKKIN
                     RLKEILADERLLLNVIKDEITIIKENYSDERRTEIRHAEGEIDMRDLISDEEIAITLT
                     HFGYIKRLPSDTYKSQKRGGRGISALTTREEDFVRHLVTTTTHSRLLFFTNKGRVFKL
                     NAYEIPEGKRQAKGTAIVNLLQLSADEKIATLIPIDGNDENEYLLLATKKGIVKKTKR
                     EEFKNINKSGLIAIGLRDDDELIGVELTDGKQEVLLVTKEGMSIRFDENDIRYMGRTA
                     MGVKGITLSKEDFVVSMNLCSKGTDVLVVSKNGFGKRTNIEEYRSQIRAGKGIKTYNI
                     SEKTGTIVGADMVNEDDEIMIINSDGVLIRIRVNEISLFGRVTSGVKLMKTNDEVNVV
                     SIAKINIEEE"
     gene            complement(4705..6606)
                     /gene="gyrB"
                     /locus_tag="KPD58_000399"
     CDS             complement(4705..6606)
                     /gene="gyrB"
                     /locus_tag="KPD58_000399"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003226808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBG0264190.1"
                     /translation="MKQEYGASQIQVLEGLEAVRKRPGMYIGSTSPRGLHHLVYEVVD
                     NSIDEALQGYCSDIYVSINEDGSVLVKDNGRGIPVEIHPKTGKSTLETVLTNLHAGGK
                     FGGGGYKVSGGLHGVGVSVVNALSKWMVAEVYLNGKIYKQTYEKGLPTSKLEVVGESQ
                     DKGTMIQFMPDETIFDEIEFKYETLEYRLRELSFLNKGIKIVFEDKREGQEKRKEFHY
                     TGGLVEYIKYLNKSRTGIHDDIVYIDKKVDDCFVELAMQYTDGYTENIYSFANNINTH
                     EGGSHLSGFKAALTKTVNDYAKRNKFLKENDVNLLGEDIREGLTAVVSVKLPEPQFEG
                     QTKTKLGNSFMRGIVDSVTVDELGSFLEENPSTARIIVDKALRAQRAREAAKKARELT
                     RRKSVLESTSLPGKLADCAEKDPSKSEIFLVEGDSAGGSAKQGRDRNSQAILPLRGKI
                     LNVEKSRLDRILSSDEIKNMITAYGCGIGEDFDIDKARYHKIIIMTDADVDGAHIRTL
                     LLTFFFRYMRPLIDEGYVYAAQPPLYKVTKQKKEHYVYSDKELNILLDEIGRNGVELQ
                     RYKGLGEMNAEQLWETTMNPETRTLLQVTVEDAAIADEVFSMLMGDKVAPRKEFIEEN
                     ARFVRNLDI"
     gene            complement(6607..7722)
                     /gene="recF"
                     /locus_tag="KPD58_000400"
     CDS             complement(6607..7722)
                     /gene="recF"
                     /locus_tag="KPD58_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBG0264191.1"
                     /translation="MKLKSLQLVNFRNYKKLHLEFNGKVNLLVGKNGQGKTNIVESIY
                     MLSFGKSFRTNKDKEMVRFNSENLYIGGSFSKYNKYSLIELIIGKDKKGIRINKVPLQ
                     KIQELLGNLNVVIFSPEDLRLVKEGPKERRAFIDKEISQIIPKYYKYLTNYNKTLSQR
                     SRVLKNIHVDEALLDVYDDTLAKYGSYIYILRRDFIKKIANISENMHMNLTNGVERLS
                     IRYKNQINITDEDTIDTVYNKFLAKLSSNRPNDIESKTTRYGIHKDDLNIFINDLDAR
                     LFGSQGQQRTASISLKLSEIELIKNEVEEYPVLILDDVFSELDEARQKLLVNNLSNVQ
                     MFITSAEVSHKKIFDEKNVTIFNIENGDVISIENGGN"
     gene            complement(7740..7946)
                     /gene="yaaA"
                     /locus_tag="KPD58_000401"
     CDS             complement(7740..7946)
                     /gene="yaaA"
                     /locus_tag="KPD58_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HBG0264192.1"
                     /translation="MTEITIESEYIKLDQFLKLAEIASTGGHAKFLIQEGLVTVNDEI
                     ELRRGKKIKSGDIVEIEGTKIKVL"
     gene            complement(8083..9189)
                     /locus_tag="KPD58_000402"
     CDS             complement(8083..9189)
                     /locus_tag="KPD58_000402"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBG0264193.1"
                     /translation="MKIICNQKILANRIGIAQKAINGKTTIELLKGILISTEEGQLKL
                     TGYDAEIGIETYVQAEIIEKGDVVVDARLFGDIIRKLPDSFVEIETDSENNIYINCVN
                     SRFKIKGYAAKEFPKLPELNEEDLYSIPQEILKNMIKQTVFAISQDQTKPVLMGELLE
                     IVDRNLNLVAIDGYRLAVKSCSVDSLTENIKVIIPGKTLIDVNSLLSGEDNVKVGFNE
                     KNAIFIINDTKIITRLLEGDFIDYKKLLPREHNSRVKLNTKELLNSIERASLLSQSEK
                     NNLIKLSIRDKVMAITSNTEKGNVYEEVEIDLDGDYLDIAFNSRYFIEGLKNIDNEEI
                     FIEFTTNVNPCIIKPTDDVNYIYLLLPVRISSNI"
     gene            complement(9431..10753)
                     /gene="dnaA"
                     /locus_tag="KPD58_000403"
     CDS             complement(9431..10753)
                     /gene="dnaA"
                     /locus_tag="KPD58_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBG0264194.1"
                     /translation="MMDIVSLWDKTLQLIKGDLTSVSFNTFFKNIVPLKIHLNDLILL
                     APSDFNKDILENRYLHLIEDAISQLSLKKYNIKFVLSEKEVADLNSDSTDLNYRVLYP
                     NLNPKYTFDTFVIGNSNRFAHAACVAVAESPAKAYNPLFLYGGVGLGKTHLMHAIGHH
                     IVSQKKDSKVVYVSSEKFTNELINSIKDDKNEEFRNKYRNVDVLLIDDIQFIAGKERT
                     QEEFFHTFNTLHEANKQIIISSDRPPKDIPTLEDRLRSRFEMGLITDIQAPDFETRIA
                     ILRKKAQLERIDVPNEVMSYIAKNIKSNIRELEGALTRVVAYSSLSNRVISFDLATEA
                     LKDIITTSKNEEINVLRIKEKVSSVFNLKMEDFNSKKRTRSIAYPRQIAMYLTRELTD
                     LSLPKIGEEFGGRDHTTVIHAHDKVSKDIEESEEIKTKIDKIISDLKG"
     gene            11409..11546
                     /gene="rpmH"
                     /locus_tag="KPD58_000404"
     CDS             11409..11546
                     /gene="rpmH"
                     /locus_tag="KPD58_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBG0264195.1"
                     /translation="MSKRTYQPKKRQRSKEHGFRKRMKTSNGRNVLKRRRAKGRNRLT
                     H"
     gene            11624..11968
                     /gene="rnpA"
                     /locus_tag="KPD58_000405"
     CDS             11624..11968
                     /gene="rnpA"
                     /locus_tag="KPD58_000405"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBG0264196.1"
                     /translation="MDFNRTKGLKKDSDFRKVYKHGKSFANKYLVIYILKNKSDYSRV
                     GISVSKKVGKAITRNRVRRLIKEAYRLNIDEKIKPGYDIVFIARVSSKDATFKDIDKS
                     IKNLVKRTDISI"
     gene            12000..12251
                     /gene="yidD"
                     /locus_tag="KPD58_000406"
     CDS             12000..12251
                     /gene="yidD"
                     /locus_tag="KPD58_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBG0264197.1"
                     /translation="MNKIIYILKEISKYLSNLCIYLVRFYQKYISPLKGPTCRFYPTC
                     SQYSIEAFKKYGFVKGMYLTIRRILKCHPFHPGGYDPLK"
     gene            12283..12990
                     /locus_tag="KPD58_000407"
     CDS             12283..12990
                     /locus_tag="KPD58_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBG0264198.1"
                     /translation="MNIIGNFLGLILKVIFEFVNHYGISIILFTILVKIILLPLTIKQ
                     TKSTKAMQDIQPRIKEIQEKYKNKPEKQNEEIVKLYGEAKINPLSGCLPLLIQFPILI
                     GLFSVLREPVAHGVFANKAAFLAADNGFLWIKSLTSPDYVLAVFSGASAYVMQKVMTP
                     KDQLQGSMKVMTYVMAGMSFYWGFIFPAGLTLYWTVSNLFSIAQYYLIMNPLKAKLAA
                     NSKEEKVNEKKPKINKK"
     gene            12959..13588
                     /locus_tag="KPD58_000408"
     CDS             12959..13588
                     /locus_tag="KPD58_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HBG0264199.1"
                     /translation="MRKSLKLIKSKSKEEAISKAIAELNLKAEDIEVEILENPSKGFL
                     GFIGAKDGTYEIFVIEREVDVAKNFIEVMLKNANVDAKVNVSQKDNLIMVDIEGKEAA
                     SLIGRRGETLDSIQFLTGLALNKINKDSHTRVLVDIENYRSKREESLIRYANKVAREV
                     AKTRKTKKLDYMNPYERRIIHSALQNDKYVITYSEGTDPYRRLVIECKR"
     gene            13700..15079
                     /gene="mnmE"
                     /locus_tag="KPD58_000409"
     CDS             13700..15079
                     /gene="mnmE"
                     /locus_tag="KPD58_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBG0264200.1"
                     /translation="MFIDDTIAAIATAPGEGGIGILRISGEKALKVAEEIFKSMSGKS
                     IEEYNKRTLIYGNIVDNENIIDEVLLAYMKGPNSYTGEDVIEINCHGGFISVKKILEL
                     ILSKDVRLAEAGEFTKRAFLNGRIDLSQAEAVIDVIKAKTDIAHEVAQNQLEGSLSKK
                     IRELRDKVTEILAHVEVAIDYPEEDIEHITYQTLKEKTDELKKDIKKLYDTAESGKIL
                     REGLKTVIVGKPNVGKSSLLNSILGENRAIVTDIPGTTRDVIEEFVNIKGIPLKIVDT
                     AGIRDTDDIVEKIGVEKSKESFTSADLIVMVLDASRKLSEEDIEILEKLKDKQTIVLL
                     NKNDLKQEIEEEKILKYVENNSIIKISALQQEGIEELQDKIESMVYKGSIKNNSSLVV
                     TNSRHKDALSKAYKSATDALIALEQSMPFDFVEVDLKNIWDYLGYINGDTVTEDLLDN
                     IFHNFCIGK"
     gene            15177..17069
                     /gene="mnmG"
                     /locus_tag="KPD58_000410"
     CDS             15177..17069
                     /gene="mnmG"
                     /locus_tag="KPD58_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBG0264201.1"
                     /translation="MKFEAGKYDVIVVGAGHAGCEAALATARMGYKTLIITMSLDSIA
                     LMPCNPSIGGTGKGQLVKEIDALGGQMGLNIDKTYIQSRMLNTAKGPAVHSLRAQADK
                     FKYHEEMKKTLEDEPNLDIAMDEVVEILHEGNVVIGVGTKLGCSFKSKAVILATGVYL
                     NSKIYMGEVAFYEGPNALGYAKYLTDSLVELGLRMRRFKTGTPARVHRDSIDFSVMSL
                     QEGDEKVTPFSFMNENIEKKQEPCYLTRTTEETQKVILDNLKRSAMYSGVIESTGPRY
                     CPSIEDKVVRFSDKTSHQLFIEPEGLNTKEMYIQGISTSLPFEVQLDMYKTIKGLENC
                     KIMRPAYAIEYDCVDPTQLKISLEIKGVENLFSAGQFNGTSGYEEAAAQGLMAGINAV
                     RKIEGKEPFVLDRSEAYIGVLLDDLVTKGTNEPYRMMTSRAEYRLYLRQDNADMRLTQ
                     KGYDIGLVKKDRYERFLNKKAAVEKEFERLKNERVTPKEVNSLLEEKGATPIKVGISL
                     YEFLKRPEVTYELLEELGKGAGEDVSREVKEQCVIITKYEGYIEKQLKQIDQFKKLEN
                     KKLDEKINYSSIEGLRLEARQKLDDIKPISIGQASRISGVSPADISVLLIYLEQIRRT
                     RGGKGE"
     gene            17062..17781
                     /gene="rsmG"
                     /locus_tag="KPD58_000411"
     CDS             17062..17781
                     /gene="rsmG"
                     /locus_tag="KPD58_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBG0264202.1"
                     /translation="MNNIELLKNGIEGFNIDTNDSMLEKFKIYREILVDWNKKMNLTG
                     IEDEKEVYIKHFLDSISAVKNGYIKNGMSIIDVGTGAGFPGIPLKICLDSLELTLLDS
                     LNKRINFLEEVSRVLELDNITFIHGRAEDFGKNEDYREKYDVATARAVAGLPILMEFC
                     VPFVKVGGYFICLKGPNANLELEESQKAIDVLGLEYIEKIDVELPEVDLNHNILVFKK
                     VMETPVKYPRKAGKPAKNPIK"
     gene            17944..18729
                     /gene="noc"
                     /locus_tag="KPD58_000412"
     CDS             17944..18729
                     /gene="noc"
                     /locus_tag="KPD58_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HBG0264203.1"
                     /translation="MEDKRVMEIPIEDVVPNPYQPRKIFSQVSLEELSNSIKVYGIIQ
                     PITVRAKDGKYELIAGERRLRAAKLAELKTIPAIINNMNDESSAVLALLENLQREDLN
                     FIEEAIGYENLIKEHAFTQQQLAEKLGKNQSTVANKLRILKLPNDIKMKLIENNLTER
                     HARAFLKLPSEDLMQSVLDKVIKNELTVKKTEKLIQDVLEETKVQEEPDKKQNIKGAM
                     SIRIYINTIKQAFDAISNTGIDAKYNEIDKGDYMEVVVKIPKK"
     gene            18864..19637
                     /locus_tag="KPD58_000413"
     CDS             18864..19637
                     /locus_tag="KPD58_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBG0264204.1"
                     /translation="MGKVIAVFNQKGGVGKTTTNVNLSASLGTLGKKILVLDLDPQGN
                     TTSGYGINKNEVENTIYEIMLDGLHIKEAIISTEFENVDVVPSATELSGAEIELTSKT
                     NREYILKNSIKAVIDEYDYIFLDCPPSLGMLTINCLTAVDSVLIPIQCEYYALEGVSQ
                     LMETIKLVKSRLNADIEIQGVVLSMFDGRANLSIQVVEEVKKYFKGSVYTTLIPRNVR
                     LAEAPSHGKPVIYYDKRCRGSVAYLELAEEFIDLEEEEW"
     gene            19639..20502
                     /locus_tag="KPD58_000414"
     CDS             19639..20502
                     /locus_tag="KPD58_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBG0264205.1"
                     /translation="MENKSKRSNRLGRGLSALIPEIKGETSEKEIVNIDIDKIYPNEV
                     QPRKQFDEEKIKVLSDSIKNYGVLQPIVVKMDENNKYMIIAGERRFRASKLANKNQIP
                     AIIKDIDMKDIMEIALIENLQREDLNSIEEALAYKSLIEHYNVTQEEISEAVGKSRPH
                     ITNTLRLLNLGQDVIEMIDSGRITAGHGKALLRIADKDLQLQIAKKIEEEELSVREVE
                     NIAKKISENKQEEPKKSKPKDVFILDVEDKLRNIFGTKVNISKGKKKGKIEIEYYNDD
                     DLNSIVSMLLE"
     gene            20535..21677
                     /locus_tag="KPD58_000415"
     CDS             20535..21677
                     /locus_tag="KPD58_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBG0264206.1"
                     /translation="MIYLDNAATTYPKPERVYNAVLDCMKNYCANPGRAGHKLAMRAA
                     REIYDTRENIAKLFNVSNPMNIVFTSNATDSLNLAIKGVLQEGDHVITTSMEHNSVIR
                     PIKALEKRGIENTVVKCDYEGFLDYEDLEKSIKSNTKLIVTTHASNVCGTLIDIKKVG
                     EIAKKHNILFLVDASQTAGVYDIDVNECNIDMLAMPGHKCLFGPQGTGILYVREGLNL
                     NILKEGGTGSKSEEIVQPELFPDKYESGTHNTPGIAGLNQGILFIFERGINNIRQHEE
                     ELCQYMIDKLEEVPDIKIYGPKDSKKRASVIALNIGDMDSGEVTFLLDSDYNIATRSG
                     IHCSPLAHTTLGTLKQGAVRFSIGYFNTKDEIDKAVEALKKISKNK"
     gene            complement(21810..22385)
                     /locus_tag="KPD58_000416"
     CDS             complement(21810..22385)
                     /locus_tag="KPD58_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HBG0264207.1"
                     /translation="MKNKKIMLVLSILSISIFAVGCTNAQNGSDSSKKETKSNTNVEQ
                     PKEENNTEKEVPNNKAKLTPKEETKAQSATIYSFDVDKTDLIENKVDLNKIDENTLFE
                     ELQKLKVVPESAKLNSFTTKDIDGVKTGILDVSSDFTKSNLGSDAETLMLDSVARTYI
                     KNMNVEQIKITVDGSNYESGHIVLEEGDYLK"
     gene            22645..23937
                     /locus_tag="KPD58_000417"
     CDS             22645..23937
                     /locus_tag="KPD58_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG0264208.1"
                     /translation="MEILSLGEKIKKLRKEKNMTLKELAGDRITAAQISHIERDKSHT
                     SYELLEYLGDKLDVSVEYLLETKEMQSKKITDNLILQSEIYIKENELNKAEDQIKEVL
                     DICKRYDLMENYGRCNFLMANINLKKSNYNDAIENFEKALYFFIKNSDSENILKCYLN
                     IGKIYMQEDFYKGAISHFNFAEEILSENKFEDVNIHKELYSKMAYCYIKLDNPNMSLK
                     YIEKINEIDNRNDRKEDVDILVLKANNMLEIGKYEESRDYFKKALKILESEDNKTGLA
                     NVYLTICDVYRKIGETDRVLEYSQKVYDIKKNDEDEYMMSGLFKIIEAYIDKEDYDLA
                     RKYCKIALASSIKNKNKFNEYKALRFYANMYKNQNEVTLAIEYLIKCIKIVSDLGNNR
                     ILANLYIDLGQLYSNISKEKELEYYQKGVFMYKNLEIM"
     gene            24042..24644
                     /gene="yedF"
                     /locus_tag="KPD58_000418"
     CDS             24042..24644
                     /gene="yedF"
                     /locus_tag="KPD58_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HBG0264209.1"
                     /translation="MSIKIDARGLACPKPVINTKRELDNLEEGVVVTIVDNETAKENI
                     LKLAKSLSCEANIVDEKENFISIEIRKGKNILDKQNVVDNKESELDNTCIFISSDKMG
                     LGNDELGKVLIKGFIYTLTESKPYPKYVLLVNGGVKLSAENEETIENLKILENMGVEV
                     LSCGTCLDYYNLKDKLQVGTVTNMYTIVETLKNASNTISI"
     gene            24717..24980
                     /locus_tag="KPD58_000419"
     CDS             24717..24980
                     /locus_tag="KPD58_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HBG0264210.1"
                     /translation="MNQMYIVSFNSTHHAIRSEKLFGENSLKVMALPTPREITASCGI
                     SIKFSFEDMEKIKTILVENNVDIKGIYCISKLENGSKEVEKLD"
     gene            24995..25180
                     /locus_tag="KPD58_000420"
     CDS             24995..25180
                     /locus_tag="KPD58_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HBG0264211.1"
                     /translation="MPMDLKIGDIVELKKQHACGCKEFEIIRTGMDIKIKCTKCSRLI
                     MLDRETLEKRVKKLIKK"
     gene            25283..26479
                     /locus_tag="KPD58_000421"
     CDS             25283..26479
                     /locus_tag="KPD58_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG0264212.1"
                     /translation="MAVKYAKRMQGLQGSEIRELLKLTQQPQIISFAGGMPAPELFPV
                     EEMKKVSVAVLEENGRSAMQYTTTEGYEPLREKIAARMNDKNKTNVNKDDILVTSGSQ
                     QGLDFAGKVFIDEGDVILCESPSYIGAINAFKSYQPKFIDVPTDSDGMIMEELEKILE
                     TTDRIKMIYVIPDFQNPTGRTWPLERRKKFMEIVNKFEIPVIEDNPYGDLRFEGETLP
                     SLKSMDTKGLVIFLGTFSKIFCPGYRLGWTCASPEILSKFNFAKQGADLQASTISQME
                     VSKFMDMYDLDAHVDKIKAVYVKRRDVMLKTMEEEFPEGLVFTHPEGGLFTWVELPSN
                     LNAKELMPKCLDKNVAYVAGGGFFPNGGRENTFRLNYSNMPEEKIIEGIKNIAAVLKE
                     AMGVEA"
     gene            26693..26971
                     /locus_tag="KPD58_000422"
     CDS             26693..26971
                     /locus_tag="KPD58_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBG0264213.1"
                     /translation="MRNYELVYVVKPNSDEEVREAILNKVKEVVATDGEIVKVDTWGT
                     KKLAYPIAKFTEGFYVLVNFKSAVDVPKEIDRNLKINENVIRHMIVVA"
     gene            27008..27442
                     /gene="ssb"
                     /locus_tag="KPD58_000423"
     CDS             27008..27442
                     /gene="ssb"
                     /locus_tag="KPD58_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG0264214.1"
                     /translation="MNQVVLVGRLTRDPELRYIPGTGTPVASFTIAIDRDYVKKDGSK
                     ETDFIPVEVMGKSAEFCANYITKGRLVALQGSIRVDNYQTQSGEKRTFTKVSTRSVQA
                     LDSKNKSENSYRESAPAFEPSFEPQGLDPQGFQAIDDDDIPF"
     gene            27461..27688
                     /locus_tag="KPD58_000424"
     CDS             27461..27688
                     /locus_tag="KPD58_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBG0264215.1"
                     /translation="MMNKKRRKKKRVCQFCADKNAKIDYKSTQRLQKYITERGKILPR
                     RISGTCAKHQRELTVAIKRARNIALLPYTLD"
     gene            27831..28151
                     /locus_tag="KPD58_000425"
     CDS             27831..28151
                     /locus_tag="KPD58_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBG0264216.1"
                     /translation="MKIDKGSEITRNIKIIEWMKTEILMSVSDLFNLLFKGVKPLDEA
                     IQDTLANIIMITYLLAKRLGISFRDVDYKVKEKIKIGIDEDHSVERWYGDLSNLKKHM
                     DNRE"
     gene            28159..29115
                     /locus_tag="KPD58_000426"
     CDS             28159..29115
                     /locus_tag="KPD58_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HBG0264217.1"
                     /translation="MNNKIRLSKAMSIIVLAIIIALVIAYIPMLGIFSMLAAVPYVII
                     GAITDRKYSLISILVTFCVLILFADISYALNICIMYSLPGIVIGKMLKRSFEQEDSNK
                     FEPIYGGTIIFVLSMLVYFFVLKTFLNVNLLDEVSKMISEIVNIQKNSFSATELKVFD
                     KMKPDEIVSYFTNMLPMMLFLQGLLSAFVTYYLSVFFIKRITKMNIRFPKFADFYLPG
                     NAILTTFLLYLLVLFIQIIGSKLYTELIMTNLQLVFNLMFVIQGIAVCIYFLKKWIRQ
                     GPNKIMFFSGIILCLFGFMGISFVGMVDSIIDFRKVRSYKST"
     gene            29126..31120
                     /locus_tag="KPD58_000427"
     CDS             29126..31120
                     /locus_tag="KPD58_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HBG0264218.1"
                     /translation="MSNKQTFKLNMPEINLYIIVIGISSIILLYYNLYVGCLFFCIFV
                     YMVFHNWRTTNIRRHEWTEYIQNLSLDIDETTKKAIINLPIPLCILEFDGNISWYNGK
                     FYDMIGQKDLLDKNIEDIVKNLNLRKVLNENKEMYTEINYKEKEYTIIYNVIKNDQEK
                     NPKYLMILYWIDKTEYLKVKQNYDDEKNAMMLIQVDGYDEVLKSAAEDKRALINVEVE
                     KILSALELNSNGALRRTSKDKFFLVMHKKELKKLEAEKFSILDTIRHIDYGNNLPVTI
                     SIGIGIDGDTLNENLKLATGALDLALGRGGDQAVVKTKDKFVFYGGKSKAVEKKTKVK
                     SRLIGHALREVIQQSDQVYIMGHKYPDMDAMGAAVGVYDICKSCNKTANIVLQSVNES
                     IEIFINKINENNYYKKLFIGKEEAIDNCTKNTLVVVVDTHRPNYTECEELLKLSEKVV
                     VIDHHRRGVEFINDAVLLFHEIYVSSTCEMVTELVQYMDEDVTINKLTAEGLLAGISL
                     DTKNFAFKTGVRTFEAASYLRKVGADTIEVKKFFNSDVKDFIIKAEIIQSTKIINNRI
                     CLAYSSTEIDSINVIIAQTADELLNIKEVEASFVLGEKDDTIFISARSLGQINVHVLM
                     EKLGGGGHIDIAGAQLKNVSLKEAYKMVNKIIEEYLEEEE"
     gene            31121..31570
                     /locus_tag="KPD58_000428"
     CDS             31121..31570
                     /locus_tag="KPD58_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBG0264219.1"
                     /translation="MKVILLKDVKGTGKKGEMKEVSDGYARNFLFPKKMAVQADSVAI
                     KELNEKNKSKEIKAQKEYEEAVLLGKQMEEINIEIYSKAGEGGRLFGSITSKEIAEQL
                     KKQKDIDVDKRKILLDEPIRSLGSTFVEIKIHQKVTTKIRVDVKEKQ"
     gene            31589..32917
                     /gene="dnaB"
                     /locus_tag="KPD58_000429"
     CDS             31589..32917
                     /gene="dnaB"
                     /locus_tag="KPD58_000429"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBG0264220.1"
                     /translation="MEDMTRIPPHSVESEQSILGSILLDKDAIITVTETIKPDDFYKE
                     AHKIIYECMITLSNKGEPIDLITLTEELRKQGHLNDIGGISYITSLSTIVPTTSNVKY
                     YADIVKEKSVLRKLIKASNEIINLGYSGATKIEDVLEQAEKSIFDISQEKTSDDFKSI
                     NLVLMDAYDMIEKLYTNKSDVTGITTGFKDLNKKINGLQRTDLILIAARPAMGKTAFS
                     LNLVQNAALKGDASVAVFSLEMSKEQLVQRMLSSQSSVELKKIKTGTLNDNDWPRIID
                     AMAVLSDAKIHIDDTPGIKISELRSKCRKLKIEKGLDLVLIDYLQLMEGEGNNESRQQ
                     EISKISRSLKILAKELNCPVVALSQLSRAPEQRADHRPMLSDLRESGAIEQDADIVMF
                     LYRDEYYHADSESKNIGEVIIAKNRHGETGSVELVWLGEVQRFGDKLRDL"
     gene            33122..33430
                     /locus_tag="KPD58_000430"
     CDS             33122..33430
                     /locus_tag="KPD58_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264221.1"
                     /translation="MEKISLKYIYPNIIKVLDEINLFRVIDNNLRESIVVYANNVDNQ
                     YHINMTNTNFGNIINICKLEKLLDVDKFMEKVIKYEKEIIEKEEFSKIEEYMLNIGEY
                     "
     gene            33512..34801
                     /locus_tag="KPD58_000431"
     CDS             33512..34801
                     /locus_tag="KPD58_000431"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBG0264222.1"
                     /translation="MKTVAIVGSQWGDEGKGKVIDYLATQADVVVRGQGGNNAGHTLV
                     VEGKKYALHLIPSGVLNPNTVNIIGNGIVFDPKGFLEELEMFKTDNISTENIKISDRA
                     HVIFPYHKELDALSEEARGDLKIGTTKKGIGPCYMDKTERSGIRICDLMDKDKFAIKL
                     KAQIDAKNEIVKNIYGKEELFDFETIYNEYLGYAEQIRKYVADTSVIVYDAVRAGKKV
                     LFEGAQGTLLDLDLGTYPFVTSSHPTSGGFAIGAGIGPNMIKDVVGIVKAYTTRVGEG
                     PFVTEQINETGDKIREQGHEFGVTTGRPRRCGWFDAVIVKYAARVNGLTSISFMLLDV
                     LTGFDKIKVCTSYKMGDKIITDFPASLDDLAKCEPVYEELDGWNEDITQIDNFDDLPE
                     NAKKYVAKIEELVGVSVDMVSVGPNRAQTIIRRNIFA"
     gene            35127..35202
                     /locus_tag="KPD58_000432"
     tRNA            35127..35202
                     /locus_tag="KPD58_000432"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35160..35162,aa:Lys,seq:ttt)
     gene            35209..35283
                     /locus_tag="KPD58_000433"
     tRNA            35209..35283
                     /locus_tag="KPD58_000433"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35242..35244,aa:Glu,seq:ttc)
     gene            35288..35363
                     /locus_tag="KPD58_000434"
     tRNA            35288..35363
                     /locus_tag="KPD58_000434"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35321..35323,aa:Val,seq:tac)
     gene            35397..35471
                     /locus_tag="KPD58_000435"
     tRNA            35397..35471
                     /locus_tag="KPD58_000435"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35429..35431,aa:Thr,seq:tgt)
     gene            complement(35854..36846)
                     /locus_tag="KPD58_000436"
     CDS             complement(35854..36846)
                     /locus_tag="KPD58_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0264223.1"
                     /translation="MNEDKIRKILAKYAKRRDDNELLLEHRKNEVYNRIPEIKSIDDE
                     ISKIGLSLAKIVLLNPKSKDEIVKKTKENIESLKVKKERLLAESNIPLDYLEIKFQCI
                     SCKDKGFLPNGEKCSCLKQEIVNEAYKMSNLDRILSQENFSNFNLNIFSPKKGSDGEI
                     SPRENMLNNLSICENFVHDFKKDNSENLLFYGSTGLGKTYMCNCIAKELLDKGNVVIY
                     QTSFRILDILEDYKFRRDTNNQISEDNYKNLFDCDLLIIDDLGTELNNSFTSGEIFNI
                     VNTRLVAGKKIIISTNLTPSQIGNTYTQRTLSRILDKFRILEFTGDDLRWERFK"
     gene            complement(36855..37913)
                     /locus_tag="KPD58_000437"
     CDS             complement(36855..37913)
                     /locus_tag="KPD58_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBG0264224.1"
                     /translation="MFFKESNEIDLGEITIPNIFIDIFMPMADGLYVKVYLLGYRQAC
                     DITSNPKFDNNSIAKNLNIPLSDVLSAWKFWEEKKIIKIHDNGEYDNFNYSIEFLDLK
                     NFYIENILSNNSSIKSNTDKVVSTSENPSIRKMFNSINKIVGRYLDPSEKMSIMDIMN
                     KYNMSPDMILCAYEYVKDKTGTSKPVKYIEGIIRNWYDSNLYTPKDVEESFLVRSERY
                     ILYKTIFNELGFSRQPSKSEKELMDTWFDKFNMDIDLIINACSKSKNISNPSISYING
                     IIKNWNEKNIKNLNDLKQKEEERIVKENINKKQINTTQNNNTYKKTKFHNFNETFTQY
                     TSDELDEIIKKSQKEKFK"
     gene            38038..39501
                     /locus_tag="KPD58_000438"
     CDS             38038..39501
                     /locus_tag="KPD58_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HBG0264225.1"
                     /translation="MVIKFNQKVRRTLSVAILFMVLIIGCFIEKDDSLETYKEKTNSK
                     INQYNMDVIFDDETKRLMCNQNVDYINNTKSNLDKIYFHIYPNAFSKKDFAPFEKSEM
                     TRAYPNGFNEGYIDIKNILNNNSKMEYKIKGDKNDILEIELGKELKPNERMSLDIKYN
                     VKIPNSIGRFGYGENTINVTNWFPIACVNDERGWNLKSYETIGDPFYSETSDFHVKLL
                     IPNKYKIAHTGKLIDDKHDNKKMLYEIEAENVRDFAFILSSKFNVNKVDSKGVAINTY
                     NLNKKLSKKATEVAKDSINIFSELFGKYPYKEFSVVASDFFIGGMEYPMLVMIDQSLY
                     NEKNEFLLEYVIAHETAHQWWYSAVGNDEISEPWLDEALTEYSTIVYFEQKYGKDMAN
                     KLIKTMEIQTKSYLSENIFKPANEYKNSTEYSLNVYTKGAIAFNEIRKEVGDKVFFET
                     LNEYYNKYKHKNANGRAFVELWNSKGVDINKIISECK"
     gene            39603..40400
                     /locus_tag="KPD58_000439"
     CDS             39603..40400
                     /locus_tag="KPD58_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0264226.1"
                     /translation="MLKYCSVGSGSSGNCHYIGYKNTNILVDAGLSGKRITTGLKDIG
                     VDADKLKGIFITHEHVDHIKGAGILSRKFDIPVFANIKTWCSMKDKLGDIKDKNMKVF
                     ENDKTYSLGDIIVRPFSIEHDASDPVGYNFYTESDEKMSIATDIGCITQNIKKHLYKS
                     KLVVLESNYDPNMLMMGSYTYALKKRVMSSTGHLSNEDAANFCVELINEGTESILLAH
                     LSKENNFPELAYETSKGVLASNDIVVGQDVKLDVLSRNDVSNVYEMK"
     gene            40413..42515
                     /locus_tag="KPD58_000440"
     CDS             40413..42515
                     /locus_tag="KPD58_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC/EAL
                     domain-containing protein"
                     /protein_id="HBG0264227.1"
                     /translation="MLKRNFENVEDNKILYSIKQGMILAIPAIMTGSTALVILNLPIK
                     AYQEYLSGLFNGEVTNILNFINDSTLGIISLIILLTISYSYGKIYGSKYTVLVPIVAM
                     CSFLVFSHGNELNSYIEIFKTKWLFTSIIVSMTSSVLFVKLTESFVTSVKFHTEGADA
                     DFNMVVSAIVPFIIVVFLFSIFRVIMISLIGSSNFQDIFYNLFSNVFNKMGTNLMSAL
                     LFIFLMHFMWFFGIHGSNVLDTVAKNLFENSMAININLVNNNQLPTEIFTKTFFDTMV
                     LLGGCGSLLCLVIAIFLSEKRINVRKLAKIASIPALFNINEMLVFGIPIVFNYIMFVP
                     FVITPIILTITSYVAMSTGIVPCTVSAVEWTSPIFLSGYMSTGSIRGSLLQVFNLCVG
                     VMIYIPFIKMSQNRYTYMFKNNIENLTNLLKRYELTGEQPNLLNHNGKIGSISKMLAS
                     DLKFAMKRGEIELFYQPQVNYNGNVVGAEGLLRWKHSIGGFIYPPLVIILAKEEGFLD
                     ELGEYIINKACIDLKKSEKLLRNPVKFSVNISPDQLDDPKLPEQIKNIISSNDINPNM
                     LGIEITEQIALSGSQIIIERINAIHNLGIKLIMDDFGMGHSSLIYLQNNNFDIVKLDG
                     SLVKEILTNKRSSEIIMTIVNLSKNLDFDIVVEYVENIDQRDKLYGLGCEIYQGYYYS
                     KAIPFEEFVEYANQEREI"
     gene            42583..43062
                     /gene="rlmH"
                     /locus_tag="KPD58_000441"
     CDS             42583..43062
                     /gene="rlmH"
                     /locus_tag="KPD58_000441"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBG0264228.1"
                     /translation="MNISIVVVGKIKEKYLKLGIDEFKKRLSKYCKLEIIELDDEKAP
                     ENLSVKEMEIIKDKEGKKILGKIKHNSYVIALAIDGKGLSSEELAKTMSDLAVRGNSS
                     LCFIIGGSLGLSDEVLGRADYRLSFSRMTFPHQMMRLILLEQIYRAYRINNGEPYHK"
     gene            43129..>43282
                     /locus_tag="KPD58_000442"
     CDS             43129..>43282
                     /locus_tag="KPD58_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264229.1"
                     /translation="MDRLDIFIENYDFKEYLEKLPRELIEENYKDFVIEFWKNPSVDI
                     IKLKSKY"
BASE COUNT        16285 a         5481 c         6868 g        14648 t
ORIGIN      
        1 atataaatta acctactgtt taattttcaa tgttcgtgtt tatttcttgt tccttttttc
       61 aaggacaagt aatactatac catctttaaa acagtaggtc aatacttttt ttatattttt
      121 ttataaattt ttttcatatt cttttttcag cttctctact acctttttct ctagtctaga
      181 aacagtcatc tgtgatatcc ctagttcttt agctatacta gattgtgttt tatcaaagaa
      241 aaatctatct ttaaaaattt taacttctaa ttcatttaaa gtttcaataa acttattaat
      301 aaaatcttca tactctatac ttccaaagtt accttcttct tttccaatct tatcaataag
      361 agttatatct ttatcctctg aatcatcatt acttgatgaa tccaaagaca ttggttgata
      421 tccataagaa gcctccatag cctctaaaac atcttcttca ctaacgccta tataatcagc
      481 tatatctttt acctttggat gcttcttatt ttcttgttct aatttaatct tcgcatcact
      541 tatctttttg cttaattcct gtattcttct tgggactcta agtgtccata ccttatctct
      601 aaaatacttt tttatttctc ctactatagt tggtgttgca aagctggaga actcaaatcc
      661 tttctcaaca tcatatctat caatagcata aattaatgcc aatgaagcaa cttgatatat
      721 atcttcaaaa tcaacacctt tgtttatata cttcttagct aacaatcttg ctaaatacag
      781 atgtctttca ataagaatat ttctaatgtc tacattttta tttttattat ataaattaaa
      841 tagctctttt gtatccatat ttaggtaatg tgtagcatta gctacatttt tcattaaata
      901 tcaactccta aatatttagt catttttatt tttgtccctt gattatgttc tgattcaata
      961 tctacatcat ccattaaagt tttaattata aacaatccaa gaccactctc ttttggattc
     1021 tctaaatctg gtgtacttat tgagctaaca tcatatcctt ttccattatc ttgaatttct
     1081 atattaattg cattatctaa aatattaaat actatattaa acacatcatc tttactgtgt
     1141 tttattgcat ttgtgcatgc ttcagatact gctaccttta tatcttctat atcatctatt
     1201 ggaaaaccca tcttattggc aattccagat gctgttaatc ttattatact aacataatct
     1261 ggatttgtag taatttccat ctttatagtc tcacaagcca aagtttatcc ctccacttta
     1321 aatattttat ctagacctgt tatagtaaaa atctttctta cgttactctt aggatttact
     1381 atgtatatct ctttttcatt tatttttaat ttttttaata cacctatcat agctcctaag
     1441 cctgttgagt ctatataatc taaatcttta agattaatct ttacatctaa catatttttt
     1501 tctattaaag catgtaaatg ttcctttagt ttatctgctg tagaaacatc aagttctcca
     1561 tcaagacaaa cattccaaaa tttattttgt gaatctaaat ttgaatcaat attcatagac
     1621 atgtaaaaac ctcctatttc cttttaataa ttttaaatat atttcctata ctcaatattt
     1681 tattaattac ataaactata tcatctattc cctttgatat tgatgatgca ttttcaacaa
     1741 tatcttgtat atgtctttca ttataatcta caatcttata tgccttttca acaaccttat
     1801 ttatgctgtt tagagtttta gctaaataaa ttgctactat aagagcagag cttcctacta
     1861 acactgctcc aatttgccac ccccatgcat tcattagtta acatctccct catcatcaaa
     1921 gcttttactt attactatat cttcttctga aatctttata tcttcttcat tattatcatc
     1981 atccactaaa tttattattc tctctttaac atcattaaat gtttcatgta gaacttcttt
     2041 tgggttttct ttgacatcat taaacttctc ttttgtttct tttcttagtt cagaaccttt
     2101 tttaggtgca aaaaacatac caaatataaa ccctaaaata gctcctaata ataaaaatcc
     2161 acctttttta caacttcttt tattgcacat aacaattact cctttcaatt ttaaattttt
     2221 atctaactac attatacaaa aagaatagga tatttatcct attcttcttc aatatttatt
     2281 ttggcaatcg aaactacatt gacttcatca ttcgtcttca ttaatttaac accacttgta
     2341 actcttccaa acagtgatat ttcattgact cttattctaa taagaactcc atcagaattt
     2401 ataatcatta tctcatcatc ttcgttgacc atatctgcac ctacaattgt accagttttt
     2461 tcagatatat tataagtttt aattccttta ccagctctta tttgacttct atactcttct
     2521 atatttgttc tctttccaaa accattctta cttacaacta acacatctgt acctttactg
     2581 caaaggttca tagacacaac aaaatcttct ttacttaaag ttataccttt tacacccatt
     2641 gctgttctac ccatatatct tatatcattt tcatcaaatc ttatagacat accttcttta
     2701 gttactaaaa gtacttcttg ttttccatct gtaagttcta ctccaataag ctcatcatca
     2761 tctcttaaac ctattgcaat aagaccagat ttatttatat ttttgaactc ttctctctta
     2821 gtctttttaa caataccttt tttcgttgca agtaataaat attcattttc atcattacca
     2881 tcaataggta ttagagtagc aattttctca tctgctgaca attgaagtaa attcactata
     2941 gcagtacctt tagcctgtct cttaccttct ggtatttcat atgcatttaa tttaaatact
     3001 ctacctttat ttgtaaagaa taatagtcta ctatgagtag ttgtagttac caagtgcctt
     3061 acaaaatctt cttctctagt tgtaagtgct gaaatacctc ttccacccct tttttgactc
     3121 ttataagtat cagatggaag cctttttata tatccaaagt gagtaagagt tattgctatt
     3181 tcttcatcac ttataagatc tctcatatct atttcgcctt cagcatgtct tatttctgtt
     3241 cttctctcat cagagtaatt ttcttttatt attgtaattt catcctttat tacatttaaa
     3301 agtaatcttt catcagctaa aatctctttt aatctattta tcttcttgat taaatcttca
     3361 tattcagctt ctatcttatc tctttctaaa cctgtaagtc tttgaagtct catatctaat
     3421 atagcttgtg cttggatttc agttaatttg aatttttcta ttaaacctag cttagcttct
     3481 tgcccagtct ttgaagctct tatcaagctt ataacagcat ctatattatc taaagcaatc
     3541 tttaatcctt ctaaaatatg tgctcttgct tcagctttat ttagttcaaa tttagttctt
     3601 cttgtaacaa catcttcttg atgtttaata taatgatata atatttgttt aagatttaaa
     3661 actcttggct gaccatctac aagtgcaagc attattatac taaaagtatc ttccatttga
     3721 gaatgtttat acaaattatt taatactata ttagcattag catccctctt taattctata
     3781 acaattctca taccatttct attactttcg tctcttaggt ctgatatacc ttctattctc
     3841 ttttctttaa ctaactctgc tattctttca acgagtttag ccttatttac ttgataaggt
     3901 atttctgtaa ctattatttg ctgttttcct tttggtagct cttctataaa agctctagac
     3961 ctaactttaa cttttcctct tccagttctg tatgcttctg ctatactttc ttttcccatt
     4021 ataattgcag ctgtagggaa atctggtcct tgaacaaatt ttattaaatc atctacacta
     4081 cactctggat tatctatcaa atatacagtt gcatcaatta cttctgctaa attatgtgga
     4141 ggtattgaag ttgccatacc aacagctata ccatttgagc catttactaa taaattagga
     4201 tatctagctg gcaatactga aggctctttt aacgactcat caaagtttgg tttaaagtct
     4261 acagtttcct tttcaatatc tctcagtagt tctaatgata atttactcat tttagcttct
     4321 gtataacgca tagcagctgg tgaatcccca tcaacagaac caaagttacc atgaccatct
     4381 actaaaagtg ctctagttga aaaatcttgt gccattctta ccatagcata ataaacagca
     4441 gtatctccat gaggatggta cttacctaaa acgtccccaa caatacgagc tgacttccta
     4501 tatggtttat ctggagttaa atttaactca ctcattgaat ataatattct tctatgaact
     4561 ggctttaaac catctctaac atcaggaaga gcacgtccag ctataacact cattgaataa
     4621 tcaatatacg attttttcat ttcttccgct atttcaatag ggagtatttt gttattttct
     4681 tccatgtata ctaatcccct ctctctatat atctaagttt ctaacaaatc ttgcattttc
     4741 ttctataaat tcttttcttg gagcaacctt atcacccata agcattgaaa atacttcatc
     4801 tgcaattgca gcatcttcta cagtaacttg taataaagtt cttgtctcag gattcatagt
     4861 tgtttcccat aattgctctg cattcatctc tccaagacct ttgtaccttt gaagttcaac
     4921 accatttctt ccaatttcat ctaataaaat atttaactct ttatctgagt aaacataatg
     4981 ttccttcttt tgctttgtca ctttatataa aggtggctgt gctgcataaa catacccctc
     5041 atctataaga ggtctcatat atctaaagaa gaatgttaat aataaagttc ttatatgggc
     5101 tccgtctaca tcagcatcgg tcataattat aattttatga tacctagcct tatctatatc
     5161 aaaatcttct ccaataccac aaccataagc tgttatcata ttttttattt catctgaaga
     5221 tagtatccta tctagtcttg atttctcaac attaagtatt ttccctctta atggaagtat
     5281 agcttgacta tttctatctc taccttgttt agctgaacct cccgctgaat ccccttcgac
     5341 taagaatatt tcacttttag atggatcttt ttctgcacaa tctgcaagtt ttccaggtaa
     5401 agatgtactt tctaatacac tttttctcct tgttaattct cttgcttttt ttgcagcttc
     5461 tctagctctt tgtgctctta aagctttatc aactatgatt cttgctgttg atgggttttc
     5521 ctctaggaaa gaccccagtt catcaactgt tacactatca actatacctc tcataaagct
     5581 attacctagc tttgttttag tttgaccttc aaattgaggt tctggtaatt taactgatac
     5641 tactgctgta agaccttctc ttatgtcttc accaagtagg ttaacatcat tttcctttaa
     5701 aaatttattt ctcttagcat aatcatttac agttttagta agagctgctt taaatccact
     5761 taaatgagaa ccaccttcgt gtgtatttat attgtttgca aaagaatata tgttctctgt
     5821 ataaccatct gtatattgca tagctagttc aacaaaacaa tcatcaacct ttttatctat
     5881 ataaactatg tcatcatgta taccagttct agatttattt aaatacttaa tgtattctac
     5941 caaaccacct gtatagtgaa attcttttct tttttcttgt ccttctcttt tatcttcaaa
     6001 tactattttt attcctttat tcaagaaaga aagttctcta agcctatact ccaatgtttc
     6061 atatttaaac tctatttcat caaatattgt ctcatctggc ataaattgaa tcattgttcc
     6121 tttatcttga gattctccaa ctacttcaag cttagatgtt ggcaatcctt tctcatatgt
     6181 ctgcttatat atctttccgt ttaaataaac ctctgctacc atccattttg acaatgcatt
     6241 aactacagaa acaccaactc catgaagacc tccagatact ttatatcctc cgcctccaaa
     6301 ctttcctcct gcatgaagat tagttaatac tgtttctaaa gttgatttac cagttttagg
     6361 atgtatttca acaggtatac ctcttccgtt atcttttact aaaacactgc catcttcatt
     6421 aatagataca tatatgtctg aacaatatcc ttgtaaagcc tcatctatac tattatctac
     6481 aacttcataa actaagtgat gtaaacctct agggcttgta ctaccaatat acatacctgg
     6541 tctttttcta acggcttcta atccctctaa tacttgtatc tgacttgcac cgtattcttg
     6601 tttcatttaa tttcctccat tctctatgct aataacatcg ccattttcaa tattaaatat
     6661 tgtaacattt ttttcatcaa atattttttt atgtgaaact tcagcacttg ttataaacat
     6721 ttgaacattg cttaaattat ttactaaaag tttttgtctt gcttcatcta actcactaaa
     6781 aacatcatct aaaattaata ctggatattc ttcaacttca ttttttatta attctatctc
     6841 tgataacttt aatgatattg aagcagttct ttgttgacct tgtgaaccaa aaagtcttgc
     6901 atctaaatca tttatgaata tatttaagtc atctttgtgt ataccatatc tggtagtctt
     6961 agattctata tcattaggtc tattcgatga caacttggct aaaaatttat tatatactgt
     7021 atctatagta tcttcatcag ttatatttat ttgattttta tatcgaattg ataatctttc
     7081 aactccatta gtcaaattca tatgcatatt ttcagatata ttagctatct tttttataaa
     7141 gtctctacgc aaaatataaa tataacttcc atactttgct aacgtatcat catatacatc
     7201 taataaagct tcatcaacat gtatattttt aagcacccta ctcctttgag aaagtgtttt
     7261 attataattg gtaagatatt tataatattt aggtataatc tgactaatct ctttgtctat
     7321 aaatgccctt ctttctttag gaccctcttt aaccaacctt aaatcctcag gagaaaatat
     7381 aacaacattt aagttgccaa gtagctcttg tatcttttgt aaaggaactt tatttattct
     7441 tatccctttt ttatcttttc caataattag ctctattaga ctatatttat tatattttga
     7501 aaaacttcct ccaatatata agttttcact attaaatcta accatttctt tatctttgtt
     7561 agtcctaaat gattttccaa aagaaagcat ataaatagat tctactatat ttgtctttcc
     7621 ttgaccattt ttaccaacaa gtagatttac tttcccatta aattccagat gcaacttttt
     7681 ataatttcta aaattaacaa gttgcaaact tttaagtttc acaaaaagtt ctcctttttt
     7741 tacaatactt ttattttagt tccttctatt tcaactatat ctcctgattt tatctttttt
     7801 cctcttctta attctatttc atcatttact gtcactaaac cttcttgaat taaaaatttt
     7861 gcatgacctc ctgttgatgc aatctctgct aatttaagaa attgatccaa ctttatatat
     7921 tctgattcta tagttatttc agtcataata gttcctcctt gtcactattt taaacaaatt
     7981 cctattgata cactaaaagt atattatacc ataaatatgc ttataaaagg agttgtctaa
     8041 gattagaaaa attttctaca aaagacaact ccttcatgtc tgctatatat ttgatgatat
     8101 tcttactgga agcagtaagt aaatataatt aacatcatct gttggtttaa ttatacaagg
     8161 atttacattt gtagtaaatt ctataaatat ttcctcatta tctatatttt tcaaaccttc
     8221 gataaaatat ctagaattga aagctatatc taggtaatct ccatctaaat ctatttcaac
     8281 ttcttcatac acatttcctt tttctgtatt agaagtaatt gccattactt tatctcttat
     8341 agataacttt ataagattat ttttttctga ttgagacaat aaagacgctc tctctatact
     8401 atttaaaagt tccttagtat ttaatttaac tctgctgtta tgttctcttg gcaataattt
     8461 tttataatca ataaaatctc cttcaagcaa tcttgtaata attttcgtat cattaattat
     8521 aaaaatagca tttttttcgt taaatccaac tttaacatta tcctctccag aaagcaaact
     8581 atttacatct ataagtgtct ttccaggaat tatgactttt atattttcag ttaaactatc
     8641 aaccgaacaa ctttttacag ctaatctata tccatcaatt gctactaaat ttaagtttct
     8701 atctacaatt tctaaaagtt ctcccattaa aacaggtttt gtttggtcct gtgagatagc
     8761 aaatacagtt tgtttaatca tattttttag gatttcttga ggtatgctat ataaatcttc
     8821 ttcatttaat tctggtaatt taggaaattc ttttgctgca taacctttaa ttttaaatct
     8881 tgaatttaca caatttatgt aaatattatt ttctgaatca gtttctatct caacaaagga
     8941 atctggtaat tttctaatta tatctccaaa tagccttgca tctacaacaa catcaccttt
     9001 ttctatgatt tctgcttgta cataagtttc tattcctatt tcagcatcat atccagtaag
     9061 ttttaattga ccttcttctg tagatataag tatacctttt aataattcta ttgttgtttt
     9121 tccattaata gctttttgag caattccaat tctatttgct aggatttttt gattacaaat
     9181 tattttcaat gtggataacc tcctctggct ttttatatat aaaaataagt taaaatcata
     9241 gtagtaatag tagtaggtgt tgttgataat gttaataagt atgtataaca ttataaatac
     9301 aaagatatta acatgtgtat aagatgttaa tatctttgtt attttagact tgataatttg
     9361 ttaataattt ttgttcaaaa aaagtattaa caggttattc aagagatata aacatacaat
     9421 tgtgtataaa ttatcccttc aaatctgata taattttgtc tattttagtc ttaatttctt
     9481 cactttcttc tatatcctta gatactttgt catgagcatg aattactgtt gtatggtctc
     9541 tacccccaaa ctcttcacct attttaggaa gggataaatc agtaagctcg cgcgttaaat
     9601 acatagctat ctgtcttgga taagctattg aacgtgttct ttttttagaa ttaaaatctt
     9661 ccatctttaa attgaataca gaagatactt tttcttttat tctgagaaca tttatttctt
     9721 catttttaga agttgttata atatctttta aagcttctgt agcaagatca aaagatatta
     9781 ccctattgct aagagaagaa taagcaacca ctctagttaa tgcaccttct agttccctta
     9841 tgtttgactt aatatttttt gctatatatg acataacctc atttggcaca tctatacgtt
     9901 ctagttgagc cttttttcta agtatagcaa ttcttgtctc aaagtcaggt gcttgaatat
     9961 cagttataag tcccatttca aatctcgatc ttagcctatc ttctagtgta ggtatgtctt
    10021 ttggcggtct atcactagat ataattattt gtttgtttgc ttcatgaaga gtattgaatg
    10081 tatggaaaaa ctcttcttga gttctttctt ttcctgctat aaattgaata tcgtcgatta
    10141 gtaatacatc tacgtttcta tatttattcc taaattcttc gtttttatcg tcttttatag
    10201 agtttataag ttcattagta aatttttctg aagaaacgta aactactttt gagtcttttt
    10261 tttgactaac aatatgatga cctatagcat gcattaggtg agtcttacct aatccaactc
    10321 ctccatacaa aaaaagtggg ttataagctt tagctggaga ttctgcaact gcaacgcatg
    10381 cagcatgtgc aaatctattg ctattaccaa taacgaatgt atcaaatgtg tattttggat
    10441 ttagattggg gtaaagaact ctataattta aatctgtgct atcagaattt aaatcagcaa
    10501 cctctttttc gctcaataca aatttaatat tatatttttt taatgacagt tgactaatag
    10561 catcttcaat taaatgtaaa tatctattct ctaagatatc tttattaaaa tcactaggag
    10621 ccagtaagat taaatcatta agatgtattt ttaatggtac gatgttttta aaaaaggtat
    10681 taaaacttac tgaagttaag tcacctttta ttaattgtag ggttttgtcc cataaagaaa
    10741 ctatatccat aatataaacc tccgaacaga ataattaata ttttccacaa agttattaac
    10801 agaaaagcaa aaatatatat aaattttaca gaataactaa atttatggat aatggtgttg
    10861 ataaattaat aatagtataa attattaaaa aaatatgaaa agatataaac aatatgtaga
    10921 taaagttata aataacataa acatacaata aatggtgatt ataactagag tattgtcagt
    10981 aatcttaaaa gttatacaca aaagtatcca caagctgtga ataatttcta ataagttgat
    11041 aacatttatg cacattatca acaagtatat ttataatatc aaaatatatg tcaaacttca
    11101 atgatttgta actaatttat ccacaatatt aactaattgt tgataaaaat ataaacatgt
    11161 taaaattact ttatttgtgt atataattga aaaaaataat ttagaataaa attaaataga
    11221 tttttgctag tattttcaat aaaaaaaata cataatatat atatggtaga ttttgtgata
    11281 taagcgtccc aaaaactcgt tgacaacagt agtaaaagta tttataatta tatgtgttat
    11341 gtttactttg agggtaaagt tattattttt ataaacggaa aataaaatct gaaaggcggt
    11401 gcaaaataat gagtaaaaga acttatcaac caaaaaagag acaaagaagt aaagaacatg
    11461 gtttcagaaa aagaatgaag acgtctaacg gaagaaacgt gttaaagaga agaagagcta
    11521 aaggaagaaa tagattaact cattaattac aaaagggccg ctgcaaaggt ggcctttttg
    11581 tgtaaataat tacaattcat agctgtgttt atggaggtta actatggact ttaataggac
    11641 taaagggttg aaaaaagact ctgattttag aaaagtatat aaacacggca aatcttttgc
    11701 aaacaaatat ttagtaatat atatacttaa aaataaatca gattatagta gagtaggtat
    11761 ttctgtttca aaaaaagtag gaaaagctat tactaggaat agagtaagaa gattaataaa
    11821 agaagcctac agattaaata ttgatgaaaa aattaagcct ggatatgata tagtatttat
    11881 agcaagggta tctagtaagg atgctacttt taaagacata gacaagtcta taaaaaattt
    11941 agttaaaaga acggatattt ctatttaatt gtatgctata aatatggagg ttaatgactt
    12001 tgaataaaat aatttatata ttaaaggaaa taagtaagta tttatcgaat ttatgtattt
    12061 atttagtgag attttatcaa aagtatatat ctcccttaaa gggacctacc tgtagatttt
    12121 atccaacttg ctcacaatat tcaatagagg cgtttaagaa atatggattt gtaaaaggta
    12181 tgtatttaac gataagaaga attttaaaat gtcatccttt tcatccagga ggttacgacc
    12241 ctctaaaata aaagagagta actattagga ggtataaaaa aattgaatat aataggaaat
    12301 ttcttaggat tgattcttaa ggtaatattt gaatttgtta atcattatgg tatttctata
    12361 atacttttta cgatacttgt aaagattatt ttattaccat taacaattaa gcagactaag
    12421 tcaacaaagg caatgcaaga tatacaacct cgtataaaag agatacaaga aaaatataaa
    12481 aataagccag aaaaacaaaa tgaagaaatt gttaagttat atggagaagc taaaattaat
    12541 ccactatctg gttgtttacc tttattaata cagtttccta tattaatagg attatttagc
    12601 gttttgagag aaccagttgc tcatggcgtt tttgcaaaca aagctgcatt tttagcagct
    12661 gacaatgggt ttttatggat aaaaagctta acatcaccag attatgtatt agcagtattt
    12721 tcaggagcaa gtgcatatgt catgcaaaaa gtaatgacac caaaagatca gttacaaggc
    12781 tctatgaaag taatgacata tgtaatggca ggtatgtctt tctattgggg atttatattc
    12841 ccagcaggac ttacattgta ctggacagta agtaacttat tctcaatagc tcaatattac
    12901 ttaataatga atcctttaaa agctaaactg gctgctaact ctaaggagga aaaagttaat
    12961 gagaaaaagc ctaaaattaa taaaaagtaa gagtaaagag gaagctataa gtaaagctat
    13021 agcagagttg aatttaaaag cagaggatat agaagtagaa atacttgaaa atccaagtaa
    13081 agggttttta ggttttatag gtgctaaaga tggtacttat gaaatttttg ttatcgagag
    13141 ggaagtagat gtagctaaaa attttataga ggtaatgttg aaaaatgcaa atgttgatgc
    13201 caaagtcaat gtatctcaaa aagataattt aatcatggtt gatattgaag gcaaagaagc
    13261 tgcaagttta attggtagaa gaggggaaac tttagattct attcaatttt tgactggatt
    13321 agctcttaat aaaataaata aagattctca tacaagagta cttgtagata tagaaaatta
    13381 tagatcaaaa cgagaagaat ctttaataag atatgctaat aaagttgcta gagaagtagc
    13441 gaaaacaaga aaaactaaaa aattagatta tatgaatcca tatgagagaa gaataataca
    13501 ttcagctctt caaaatgaca aatatgtaat tacatatagt gaaggaaccg atccatatag
    13561 aagattagta attgagtgta aaagataaag tcaacctcct aaacattagg gggttttatt
    13621 ttttaatatt attaaatcat taatgattgg ttaaatgtaa atctatatgt aaaaaattat
    13681 gaattaagag gtgataaatt tgtttataga tgatacaatt gcagcaatag ctacagcacc
    13741 tggagaaggt ggtataggta tacttagaat aagtggagaa aaagctttga aggttgcaga
    13801 agaaatcttt aaatctatgt ctggaaagag tattgaagaa tacaataaga gaacattaat
    13861 atatggaaat atagtggata atgaaaatat aattgatgaa gttttattgg catatatgaa
    13921 aggaccaaat tcctatacag gagaagatgt tatagaaatt aattgccatg gtggatttat
    13981 ttcggtaaag aagatattag aattaatact atcaaaagat gttagattag cagaggctgg
    14041 tgagtttact aaaagagcat tcttaaatgg aagaatagat ttatctcaag cagaggctgt
    14101 aattgatgtt ataaaagcca aaactgatat agctcatgaa gttgctcaaa atcaattaga
    14161 aggttcttta tcaaagaaga taagagaatt gagagataaa gtcactgaga tattagctca
    14221 tgtagaagtt gctatagatt atcctgagga agatattgag catataacat atcaaactct
    14281 taaagaaaaa actgatgaac ttaaaaaaga cattaaaaaa ttatatgata cagctgaaag
    14341 tggaaaaata cttagagaag gcttaaagac ggtaattgtt gggaaaccaa atgtaggtaa
    14401 atcttcacta cttaattcta ttttaggaga gaatagagca atagttactg atataccagg
    14461 aactactaga gatgttatag aagagtttgt aaacataaaa ggtatacctt taaaaattgt
    14521 tgacacagca ggtataagag atactgatga tattgttgaa aaaataggtg ttgaaaagtc
    14581 taaagaatca tttactagtg cagatttgat agtaatggtt ttagatgcat ctagaaagtt
    14641 gtcagaagaa gatatagaga tattagaaaa acttaaggat aaacaaacaa tagtgctgtt
    14701 aaataagaat gatttgaaac aagagattga agaagaaaaa atacttaaat atgtagaaaa
    14761 taatagtata ataaaaatat cagctttaca acaagaagga atagaagaat tgcaagataa
    14821 aatagagtct atggtatata aaggtagtat caaaaataat tctagtttag tagtaacaaa
    14881 ttcaagacat aaagatgcac ttagtaaagc atataaatct gcaacagatg ctttaattgc
    14941 tttggaacaa agtatgccat ttgactttgt agaagtcgat ttaaaaaata tatgggacta
    15001 tcttggatat attaatggtg atacagtaac agaggattta ctagataata tattccataa
    15061 tttctgtatt ggtaagtaat gtacaaaagt aaacttgttt ttattataga gatttttaaa
    15121 aaatttgaag tgtttctcat gaaacattaa ttttgaaaag aaaggaatta taaatgataa
    15181 agtttgaagc aggaaaatat gatgttatag tagtcggagc aggacatgct ggatgtgaag
    15241 ctgctttggc tactgcaaga atgggatata aaactttgat aataacaatg tctttggatt
    15301 ccatagcatt aatgccttgt aatccatcaa ttggagggac tggtaaaggt caattagtta
    15361 aagaaataga tgccttaggc ggtcagatgg gattaaatat agacaagaca tatatacaaa
    15421 gtagaatgtt aaatactgcc aaaggtccag cggtacactc tttaagagca caagcagata
    15481 aatttaaata tcatgaagaa atgaaaaaga cattagaaga tgaaccaaat cttgatatag
    15541 ctatggatga agttgtagaa atacttcatg aaggaaatgt tgtaatagga gttggaacta
    15601 aactaggatg ttcatttaaa tctaaagctg taatattggc cactggagtt tatctgaact
    15661 caaagatata tatgggtgaa gttgcatttt atgaaggtcc taatgcattg gggtatgcaa
    15721 aatatcttac agatagctta gttgagcttg gccttagaat gagaagattt aagacgggaa
    15781 ctccagcaag agttcataga gatagtatag acttctctgt aatgtcttta caagagggtg
    15841 atgaaaaagt aactccattc tcatttatga atgaaaatat agaaaaaaaa caagaacctt
    15901 gttatttaac aagaactaca gaagaaactc aaaaagttat actggataat ttaaaaaggt
    15961 ctgctatgta tagtggagta atagaaagta ctggtccaag atactgtcct tctatagaag
    16021 ataaagtagt aagatttagt gataaaacat cacatcaatt atttatagaa ccagaagggt
    16081 taaatactaa agaaatgtat atacaaggaa tttcaactag tttacctttt gaagttcaat
    16141 tagatatgta caaaactata aagggacttg aaaattgtaa gataatgaga cctgcatatg
    16201 ctattgaata tgattgtgtt gacccaactc aactaaaaat aagtttggaa ataaagggtg
    16261 ttgagaactt atttagtgca gggcaattta atggaacttc tggatacgag gaagcagcag
    16321 ctcaagggct tatggctggt ataaatgcag ttagaaagat agaaggaaaa gagccatttg
    16381 tattagaccg ttcagaagct tatataggtg tcttattaga tgaccttgtt actaaaggaa
    16441 ctaacgagcc ttacagaatg atgacatcaa gagcagaata tcgactatat ttaagacaag
    16501 ataatgcaga tatgagatta actcaaaaag gttatgatat aggattagtt aaaaaagata
    16561 gatatgaaag gttcctaaat aaaaaggctg ctgtagagaa agaatttgaa agactaaaaa
    16621 atgaaagagt tacaccaaaa gaagtaaata gtttgttaga agaaaaagga gcaacaccaa
    16681 taaaggttgg aatatctcta tatgaatttt taaagagacc agaggttact tacgaattgc
    16741 ttgaagaatt agggaaaggc gctggagaag atgtttcaag agaagttaaa gaacagtgtg
    16801 taataataac taaatatgaa ggttatatag agaaacaatt gaaacaaata gaccaattta
    16861 aaaaacttga gaataaaaag ctggatgaga aaataaatta ttcatctata gaaggtctta
    16921 gattagaggc tagacagaaa ctagatgata taaaacctat ttcaataggg caagcatctc
    16981 gtatatctgg tgtttctcca gctgatatat cagttttact tatataccta gaacaaatca
    17041 gaagaactag aggtggcaaa ggtgaataat atagaacttc tgaaaaacgg gattgaaggt
    17101 tttaatatag atacgaatga cagtatgtta gaaaaattta aaatctatag agagatatta
    17161 gttgactgga ataaaaagat gaatcttact ggaatagaag atgaaaaaga agtgtatata
    17221 aaacattttt tagactctat ttcagctgta aagaatggat atataaaaaa tgggatgtct
    17281 ataatagatg ttggtacagg tgctggtttt cctggaatac cattgaaaat atgtttagat
    17341 agcttagaac ttactttgtt agattcgtta aataaaagaa taaacttttt agaggaagtt
    17401 agtagagtac tagagttaga taatattaca tttatacatg gaagagctga agattttggt
    17461 aaaaatgaag actataggga aaaatatgat gttgctacgg caagagctgt tgctggatta
    17521 ccgattctta tggaattttg tgtaccattt gtaaaagttg gagggtattt tatttgctta
    17581 aaaggtccga atgctaattt agagctagaa gaatcacaaa aggctattga tgttttagga
    17641 ttggagtata ttgaaaaaat agatgtagag ttaccagagg tagatttaaa tcataatata
    17701 ttagttttta aaaaagtgat ggaaacacca gttaagtacc ctagaaaagc tggtaaacca
    17761 gccaaaaatc ctattaagta atgaaaaatt tattattggt aagcttaatt atttctcagg
    17821 aaataattaa gcttactgta aaaaaacaaa aataaaggaa ttattaaagt agatgcagaa
    17881 ataaatttgt atgtacatat agagaacaaa aataatttat atctaaaata agagaggtat
    17941 gttatggaag ataaaagagt tatggagata cctatagaag atgtagttcc aaatccatat
    18001 caaccaagaa aaatattttc acaagtttca ttggaggaat tgagcaattc tataaaggta
    18061 tatggtataa ttcaacctat aacagttaga gcaaaagatg gcaaatatga actaattgca
    18121 ggagaaagaa gattaagagc agctaaatta gcagagctta aaaccatacc agcaattata
    18181 aataatatga acgatgaatc ttctgctgtt ctagcgttat tagaaaattt gcaacgagaa
    18241 gacttaaatt tcattgaaga agcaattggt tatgaaaact taataaaaga acatgctttt
    18301 actcaacagc aattagcaga aaaattgggc aaaaatcaat ctactgtagc aaataagctt
    18361 agaatattaa agcttccaaa cgatataaaa atgaagctaa tagagaataa tttaacagaa
    18421 agacatgcta gagcatttct taaactacca agtgaagatt taatgcaaag tgtgttagac
    18481 aaagtcataa aaaatgaatt gactgttaaa aaaacagaaa aattgataca agatgtatta
    18541 gaagaaacta aagttcaaga agagcctgat aaaaaacaga atataaaggg tgctatgagt
    18601 ataagaattt acataaatac tataaagcag gcctttgatg ctatatcaaa tacaggaatt
    18661 gatgctaagt ataatgaaat agataaggga gattatatgg aagttgttgt aaaaatccct
    18721 aaaaaataga gattactgct taccagtaat ctttttttgt aatataagcc ttgtaatata
    18781 aattgattta tagacattat ttatacttaa aaaacaccta ttcaacattt aaaattaatt
    18841 taattcttaa aggggggaac atcatgggca aagttatagc tgtattcaat caaaagggtg
    18901 gagtcgggaa aactacaaca aatgtaaatt taagtgctag cttaggaact ttgggaaaga
    18961 aaatattagt attagattta gacccacaag gaaatacaac tagtggatat ggtattaata
    19021 aaaacgaagt agaaaataca atctatgaaa ttatgttaga tgggttacat attaaagaag
    19081 caataataag tactgaattt gagaatgtag atgtagttcc atcagctact gaactatcag
    19141 gtgcagagat agagcttaca tctaaaacta atagagagta tattttaaaa aattccataa
    19201 aagcggtaat agatgaatat gattatatat ttttagattg tccaccctca ctggggatgc
    19261 ttacaataaa ttgtttaact gctgtagata gtgttttaat tccaatacaa tgtgaatatt
    19321 atgctttaga aggtgttagt caattaatgg agactataaa attagtaaaa tcaagattaa
    19381 atgctgatat agaaattcaa ggtgttgttt taagtatgtt tgatggaaga gctaatttat
    19441 caatacaagt agttgaagaa gttaagaaat actttaaagg tagtgtatat acaactttaa
    19501 ttccaagaaa tgtaagactt gcagaagctc caagtcatgg aaaacctgta atttattatg
    19561 ataaaagatg tagagggtct gtagcttatt tggagctagc agaagaattt atagatttag
    19621 aagaggagga atggtagtat ggagaataag tctaaaagaa gtaatagatt aggtagaggt
    19681 cttagtgcat tgataccaga aattaaagga gaaacttcag aaaaagaaat agtcaacata
    19741 gatatagaca aaatatatcc aaacgaagtt caacctagaa agcaatttga tgaagaaaag
    19801 ataaaagtat tgtcggattc tataaaaaat tatggtgttt tacagccaat agtggttaaa
    19861 atggatgaaa ataataaata tatgataata gctggagaaa gaagatttag agcatcaaaa
    19921 cttgcaaata aaaatcaaat acctgctata attaaagata tagatatgaa agatataatg
    19981 gagatagcct taattgaaaa cttacagagg gaagatttaa attcaataga agaggctcta
    20041 gcatataaga gtttaataga gcattacaat gtaactcagg aagaaatatc agaagctgtt
    20101 gggaagagta gacctcatat aactaataca ctaagacttt taaatcttgg acaagatgtt
    20161 atagaaatga tagacagtgg tagaatcaca gcaggccatg gtaaggcttt attgagaatt
    20221 gcagataaag atttgcaact tcaaatagcg aaaaaaatag aagaagaaga actttctgta
    20281 agagaagtcg aaaatatagc taaaaaaata tctgaaaata aacaagaaga acctaaaaaa
    20341 tctaaaccta aggatgtatt tattttagat gttgaagata aacttagaaa tatatttgga
    20401 acaaaggtga atatttctaa aggaaagaaa aaaggtaaaa tagagataga atactataat
    20461 gatgatgatt taaatagtat tgtatcaatg cttcttgaat agaacaaaaa tataaaacag
    20521 agaggaagat tgttatgatt tatttagata atgctgcaac tacatatcca aaaccagaaa
    20581 gagtgtataa tgctgtttta gattgtatga aaaattattg tgcaaatcca ggtagagcag
    20641 gccataaact tgctatgaga gctgctaggg agatatatga tacaagagag aatatagcta
    20701 agttgttcaa tgtaagtaat cctatgaata tagtatttac atctaatgca acagattctt
    20761 taaatttagc gataaaaggt gtattacaag aaggagacca tgtaataaca acgtctatgg
    20821 agcataattc agtaataaga cctataaaag cattggaaaa aaggggtatt gaaaatactg
    20881 tagtaaaatg cgattatgag ggatttttag attatgaaga tttagaaaaa tcaataaaga
    20941 gtaatactaa actgattgta acaacacatg cgtctaatgt atgtggaact ttaatagata
    21001 taaaaaaagt tggagaaata gctaaaaaac ataatatact atttttagta gatgcatcac
    21061 aaacagcagg tgtttatgat atagacgtaa atgaatgtaa tatagatatg ttggcaatgc
    21121 caggtcataa atgcttattt ggacctcaag gaacaggtat attatatgta agagaagggt
    21181 tgaacttaaa tatattaaaa gaaggtggaa caggtagtaa atcagaagaa atagttcaac
    21241 cagagttatt cccagataaa tatgaatcag gaactcataa tactccaggt atagcaggtc
    21301 ttaaccaagg tatactattt atatttgaaa gaggaatcaa caatataaga caacatgaag
    21361 aagaattatg tcaatatatg atagacaaat tagaggaagt accagatata aagatatatg
    21421 gaccaaagga cagtaaaaaa agagcatcag taatagctct aaatatagga gatatggatt
    21481 ctggagaggt tactttctta ttagatagtg attataatat agcgactaga tcaggaatac
    21541 attgttcacc actagcacat accactttag gtactttaaa acaaggtgca gtaaggttca
    21601 gtataggata tttcaacaca aaagatgaga tagataaagc agtggaagct ttgaaaaaaa
    21661 tttcaaaaaa taaatagcat gagatttaat aatatagaaa taaaaaaggg gatatttcaa
    21721 aataaaaatt tgaatcatct ccttttttgt acaaaaaaga aaaggggtcc ctaagaacct
    21781 ctaatctatt tgataattgt tttaagaagt tactttaagt aatctccttc ttcaagaact
    21841 atatgaccac tttcgtaatt agaaccatca acagtaattt ttatttgttc aacattcata
    21901 ttttttatgt aagtacgagc aactgaatca agcattaaag tttctgcatc actacctaag
    21961 tttgactttg taaagtcaga gctaacatct aatataccag tttttacacc atcaatgtct
    22021 tttgtagtaa atgaatttaa ttttgcagat tctggaacaa ctttaagttt ttgaagttct
    22081 tcaaataaag tgttctcatc aattttattt aaatcaactt tattttcaat taaatctgtc
    22141 ttatctacat caaaagagta aatagtagct gattgagctt tagtttcttc ttttggagta
    22201 agtttagctt tgttattagg aacctctttt tcagtattgt tttcttcttt tggttgctca
    22261 acatttgtat tgctcttagt ttctttttta gatgaatcac ttccattttg agcattagta
    22321 caaccaactg cgaatataga tatagataat atacttaaca ctaacattat tttcttattt
    22381 ttcataaaaa tttctcctct cttaaaaact attcttatat ataattatag tcgttatgag
    22441 caaatatatg aatacaaaat agtaacaaat ttaaaaaatg atttatttaa attaataaaa
    22501 ctcaaaataa actaaatttt agtcattttt atgggaaatc atttaatgta cagtgaaaat
    22561 attgtaaaat aaaagtagtg tttttaaaat aaatataagt tataattata ttataaaaaa
    22621 atgtcttcaa aaggtgggaa tagtatggaa attttgagct taggagaaaa gattaaaaag
    22681 ttaagaaaag aaaaaaacat gacattgaaa gaattagctg gagataggat aacagcagca
    22741 caaataagcc atatagagag agataaatct catactagtt atgaactgtt ggaatatcta
    22801 ggcgataagc tagatgtaag tgtagaatac ttattagaga ccaaggaaat gcaatcaaaa
    22861 aagataactg ataatttaat actacagagc gaaatttata taaaagaaaa tgaattaaac
    22921 aaggctgagg accaaataaa agaagtttta gatatatgta agagatacga tttaatggag
    22981 aactatggca gatgtaattt tttaatggct aatattaatt tgaaaaaatc taattataat
    23041 gatgctatag aaaactttga aaaggcattg tacttcttta taaaaaatag tgatagtgaa
    23101 aatatactta agtgttattt aaacataggg aaaatatata tgcaggaaga cttttataaa
    23161 ggtgctataa gccactttaa ttttgcagag gaaattttgt cagagaataa atttgaggat
    23221 gttaacatac acaaggaact atatagtaag atggcatatt gttacataaa actagataat
    23281 ccaaatatgt cattgaagta catagaaaag ataaatgaaa tagataatag aaatgataga
    23341 aaagaagatg tcgatatttt ggtgttaaaa gcaaataata tgcttgagat tggaaaatat
    23401 gaagaatcaa gagattactt taaaaaagca ctaaaaatac ttgagagtga ggataataaa
    23461 actggattag caaatgtgta tttaacaatt tgtgatgttt acagaaaaat aggtgaaaca
    23521 gatagagtac ttgaatattc acaaaaggta tatgacataa agaaaaatga tgaagatgaa
    23581 tacatgatgt caggattatt taagataata gaagcatata tagataaaga agattatgat
    23641 ttagctagaa aatattgtaa gatagcctta gcttcatcaa taaaaaataa aaataaattc
    23701 aatgaatata aagcattgag attctatgct aatatgtata aaaatcagaa tgaagtaact
    23761 cttgcaattg aatatctgat taaatgcata aaaatagtat cagatttagg caacaataga
    23821 atattggcca atttatatat agacttaggt caattatatt caaatatatc aaaagaaaaa
    23881 gagttagaat attatcaaaa aggtgtattt atgtacaaaa acttagaaat aatgtagggt
    23941 aaataaacct ataagggtat agaaacaatc tataacaagc aagataatat ctatgataaa
    24001 atataaattg tatgaatatt tttaaggaga tgaatcagac aatgagtata aaaatagatg
    24061 cgagagggct tgcttgtcct aaaccagtta taaatacaaa aagagaattg gataacctag
    24121 aagaaggtgt tgtagttaca atagttgata atgaaacagc taaggagaat atattaaaac
    24181 ttgctaaatc tttaagttgt gaagcaaata ttgttgacga aaaagagaat tttatatcta
    24241 tagaaattag aaaaggtaaa aatatactag ataaacaaaa tgttgtagat aataaagaaa
    24301 gtgaattaga caatacatgt atatttatat cttcagataa gatgggactt ggaaatgacg
    24361 aattaggaaa agtgttaata aaaggattta tatatacatt aacagaatct aaaccttatc
    24421 ctaagtatgt acttttagtt aatggaggag taaaattaag tgctgaaaat gaagaaacca
    24481 tagaaaatct aaaaatactt gagaatatgg gtgtagaagt tttatcttgt ggtacttgtt
    24541 tagattatta taatttaaaa gacaaattac aagtaggtac agttacaaat atgtatacta
    24601 tagttgagac attaaagaat gcatcaaata ccatttctat ataatgtatg tatgtttaat
    24661 aatataaaac taacaataaa gattaactca aatagatagt cagaaaggat taagtaatga
    24721 atcaaatgta tatagtgtca tttaattcga cacatcatgc gataagatca gaaaagttat
    24781 ttggtgaaaa tagtctaaaa gtaatggctc ttccaacacc aagagaaata acagcaagtt
    24841 gtggaatatc aattaaattt tcttttgaag atatggaaaa gataaaaact attttggttg
    24901 aaaataatgt agatataaaa ggaatttatt gtataagtaa attagaaaat ggttcaaaag
    24961 aagtagagaa gttagattag gaggctaatt atttatgcca atggatttaa aaataggtga
    25021 tattgttgaa ttaaaaaagc aacatgcatg tgggtgtaaa gagtttgaaa tcataagaac
    25081 gggaatggat ataaagataa aatgtactaa atgttcaagg ctaataatgc ttgatagaga
    25141 gaccttagaa aagagagtaa aaaagctaat aaaaaagtga taaaaattta acacttaaaa
    25201 aatgtcaaat accgtatatt aatatccaaa attttggtat aataagaatt gaagaaaaca
    25261 ttttcatggg ggtgctttaa ttatggcagt taaatatgca aaaagaatgc aaggattaca
    25321 aggatcagaa atacgtgagc ttttaaaact tactcaacaa ccacaaataa tatcttttgc
    25381 tggtggaatg ccggctccag agttatttcc agttgaggaa atgaaaaaag tatcagttgc
    25441 agtgcttgaa gaaaatggaa gatcagctat gcaatatact acaacagaag gatatgaacc
    25501 attaagagaa aaaatagcag ctagaatgaa tgataaaaac aaaactaatg taaacaaaga
    25561 tgatatatta gttacaagtg gttctcaaca aggtctagat tttgcaggaa aagtattcat
    25621 tgatgagggt gatgtaattt tatgtgaaag tccatcttac ataggagcta taaacgcatt
    25681 taaatcttat caacctaaat tcatagatgt tccaacagat tctgatggaa tgataatgga
    25741 agaattagaa aagatactag aaacaactga tagaataaaa atgatttacg taatacctga
    25801 cttccaaaat ccaacaggaa gaacttggcc acttgaaaga cgtaaaaaat tcatggaaat
    25861 agtaaataaa tttgaaatac cagtaataga agataatcca tatggagatt taagatttga
    25921 aggagagact ttaccatctt taaaatcaat ggatacaaaa ggattagtaa tattcttagg
    25981 aactttctct aaaatattct gcccaggata cagattagga tggacttgtg cttctccaga
    26041 aattttatct aagtttaact ttgcaaaaca aggtgcagac ttacaagcat caactatatc
    26101 tcaaatggaa gtaagcaaat ttatggatat gtatgaccta gatgcacatg ttgacaaaat
    26161 aaaagcagtt tatgttaaac gtagagatgt aatgcttaag actatggaag aagagttccc
    26221 agaaggatta gtatttactc atccagaagg tggattattt acatgggtag agcttccaag
    26281 taatttaaat gctaaagaat taatgccaaa atgtttagat aagaatgttg cttatgttgc
    26341 tggaggagga ttcttcccta atggtggtag agaaaatact ttcagactta actattcaaa
    26401 tatgccagaa gaaaaaataa tagaaggtat aaaaaatata gcagctgtat taaaagaagc
    26461 tatgggtgta gaagcttaat taaaaataat aacttatata taatacaagg gatggttgtt
    26521 gccatccctt gtttgtttga aaagtatata attcacctta tactaaactt tgtccaaaat
    26581 ttaaattgac agaaataaga ataaatgata aaatgtgtag gtatgagatt ttagtctcat
    26641 ttctctgctc taagagtgta gagccgttaa gtccaaaggg aggtgaatta tagtgagaaa
    26701 ttatgaatta gtttatgtgg taaagccaaa ctctgatgaa gaagtaagag aggctatact
    26761 aaacaaagtt aaggaagttg ttgcaactga cggagaaata gttaaagttg atacttgggg
    26821 aactaaaaaa ttagcttatc caatagctaa gtttacagaa ggtttctatg tattagttaa
    26881 cttcaaatca gcagtagacg ttcctaaaga gatagacaga aacttaaaga taaatgaaaa
    26941 cgtaataaga cacatgatag ttgttgctta ataactggtt ttattataaa aaataggtgg
    27001 tgtttttatg aatcaagttg tattagtggg aaggttgact agagaccctg aattaagata
    27061 catcccaggt acaggaacac cagtggcatc ctttacaata gccattgata gagactatgt
    27121 aaaaaaagat ggatctaaag aaactgattt tatacctgtt gaagtaatgg gtaaatctgc
    27181 tgaattttgt gctaactaca taactaaagg tagattagta gcacttcaag gatctataag
    27241 agtagacaat tatcagacac aatcaggtga aaagagaact tttacaaaag taagtactag
    27301 atcagtacaa gctttggata gtaagaataa atcggaaaat tcatatagag agagtgctcc
    27361 agcttttgag ccaagctttg aacctcaagg tttagaccca caaggtttcc aagctataga
    27421 tgatgacgat ataccatttt aaaaaggagg gaaaaaagtc atgatgaata aaaaaagacg
    27481 taagaaaaaa agagtttgtc aattttgtgc tgacaaaaac gctaaaatag attacaaaag
    27541 tactcaaaga ttacaaaagt atataactga aagaggaaag atattaccaa gaagaatatc
    27601 tggaacttgt gctaagcatc aaagagaatt aacagttgct ataaaaagag cgagaaatat
    27661 agcactttta ccatatacat tagactaaaa aaatgagcct tgtgctcatt ttttattata
    27721 tgtatttata tatctaaatg aggcgagcta taaagctcgt ttttttgttt atatacttaa
    27781 atatattatt atttatgata taatattata tattaaggag ggttgtaaac gtgaaaatag
    27841 ataaaggttc tgagataacg agaaacataa agataataga atggatgaaa acagaaatat
    27901 taatgagtgt aagtgatttg tttaatttat tgtttaaagg ggtaaaacca ttagatgaag
    27961 caattcaaga tactttagca aatataataa tgataacata tctcttagca aagagattag
    28021 gaattagttt tagagatgtg gattataaag tcaaggaaaa aataaaaatt ggtatagatg
    28081 aggaccatag cgttgaaaga tggtatggag atttatctaa tttaaaaaaa catatggata
    28141 atagggagtg atattaaatt gaataataaa attagactat ctaaggcaat gtctataata
    28201 gttttagcta taataatcgc tttagtaata gcctatattc caatgctagg aatatttagt
    28261 atgttagcag ctgttccata tgtaattata ggtgctatta ctgatagaaa gtattctctt
    28321 atatctattt tggttacatt ttgtgtactg attctatttg cagacatttc ttatgcatta
    28381 aatatatgta taatgtattc tttaccaggt atagtaatag gaaagatgct aaagagaagc
    28441 tttgaacaag aggacagtaa taaattcgaa ccgatatatg gtggaaccat aatatttgta
    28501 ctttcaatgc ttgtatattt ttttgtatta aagacatttt taaatgtgaa tctattagat
    28561 gaagtttcta agatgatatc agaaattgta aatattcaaa aaaatagttt ttctgcaact
    28621 gaacttaaag tgtttgataa aatgaagcca gatgagattg taagttattt tacaaatatg
    28681 ctaccaatga tgttattttt acaagggttg ttatcagcgt ttgtcacata ttatttgagt
    28741 gtatttttta taaaaaggat tacaaagatg aatataagat ttcctaagtt tgctgatttt
    28801 tatttaccgg gaaatgctat actcacaact ttcttacttt atttattagt cttatttatt
    28861 caaattattg gttcaaaatt atatacagaa ttaataatga ctaatttaca gctagttttt
    28921 aatttaatgt ttgtaattca aggtatagca gtttgcattt atttcttaaa gaaatggata
    28981 agacagggac caaataaaat tatgtttttt tcagggataa ttttatgttt atttggattt
    29041 atgggaatat cttttgttgg tatggtagat agtataattg actttagaaa ggtaagaagc
    29101 tataaatcca cttaggagga tagaaatgag taataaacaa acctttaaat taaatatgcc
    29161 agaaataaat ttatatataa ttgttattgg tatttctagt ataattttgc tttattacaa
    29221 cttgtatgta gggtgtttat ttttctgtat ttttgtatat atggtttttc acaattggag
    29281 aactactaat attagacgtc atgaatggac agagtatatt caaaatttat ctttagatat
    29341 agatgaaaca actaaaaaag ccataataaa tcttccaata ccattatgta ttttagaatt
    29401 tgatggaaat atatcctggt ataatggaaa gttttatgat atgattggtc aaaaagattt
    29461 acttgataaa aatatagagg atatagttaa aaatcttaat ttaagaaaag tattaaatga
    29521 aaataaagag atgtacacag aaataaacta taaagaaaaa gaatatacaa taatttataa
    29581 tgttataaaa aatgatcaag aaaaaaatcc taaatatttg atgatattat actggataga
    29641 caagactgaa tatttaaaag taaaacaaaa ttatgatgat gaaaaaaatg caatgatgct
    29701 aatacaagta gatggatatg atgaagtatt aaaaagtgca gcagaagata agagagcttt
    29761 aattaatgtt gaagtagaga aaatactttc tgctttagaa ttaaattcaa atggtgcatt
    29821 gagaagaact tcaaaagaca aattcttctt ggtaatgcat aaaaaagagt taaaaaaatt
    29881 ggaagctgag aagttttcta ttttagatac aataagacat attgattatg gaaataatct
    29941 tcctgttact ataagtatag gaattggtat agatggagat acattaaatg agaacctaaa
    30001 acttgctacg ggtgctctcg atttagcact tggtagaggt ggtgaccagg ctgttgtaaa
    30061 aacaaaagat aaatttgtgt tttatggagg aaaatctaaa gctgtagaaa agaaaaccaa
    30121 agtaaaatcc agattaatag gtcatgcttt aagagaggtt atacaacaaa gtgaccaagt
    30181 ttatataatg ggacataaat atcctgatat ggatgctatg ggagcagcag ttggtgttta
    30241 tgatatatgt aaatcctgta acaaaactgc aaacatagtt ttacaatctg taaatgaatc
    30301 tatagaaata tttataaata aaataaatga aaataattac tataaaaagt tgtttattgg
    30361 aaaagaggaa gctattgaca actgcactaa aaatacatta gttgtagtag ttgatactca
    30421 tagaccaaat tatactgaat gtgaagaact gttaaagcta tcagagaagg tagtagttat
    30481 agaccatcat agaagaggtg tagagtttat aaatgatgca gtgcttttat tccatgagat
    30541 atatgtatcc tcaacgtgtg aaatggttac agagttagtt cagtatatgg atgaagatgt
    30601 aacaataaat aaacttacag ctgaagggct tttagcaggt ataagtctag atactaaaaa
    30661 ctttgcattc aaaacaggtg ttagaacttt tgaagctgct tcttacctta gaaaagttgg
    30721 agcagatact atagaggtaa agaaattctt caattctgat gtaaaagatt tcataataaa
    30781 agctgaaata attcaaagta ctaaaataat taataataga atatgtttag catattcaag
    30841 tactgagata gatagtataa atgttataat agctcaaact gctgatgagt tattgaatat
    30901 caaagaagta gaagcttcct ttgtattagg tgaaaaagat gatactatat ttataagtgc
    30961 gaggtcttta ggacaaataa atgtacatgt acttatggaa aaattaggag gcgggggaca
    31021 catagatata gctggagcac agttaaaaaa tgtttctcta aaagaagctt ataagatggt
    31081 aaataaaata atagaggagt atttggagga ggaagaatag atgaaagtta tattattaaa
    31141 agacgtaaaa ggaactggta aaaagggaga aatgaaagaa gtaagtgatg gatatgcaag
    31201 aaatttctta tttcctaaaa agatggctgt gcaagcagat agtgtagcaa taaaagaatt
    31261 aaatgaaaag aataaatcta aagaaataaa agcacaaaaa gagtatgaag aagcagtttt
    31321 attaggtaag caaatggaag aaattaatat agaaatatat tcaaaagcag gagaaggtgg
    31381 aagattattt ggttcaataa cttctaaaga aattgctgag caattaaaga aacaaaaaga
    31441 catagatgta gataaaagaa aaatattatt agatgaaccg ataagatctt taggttcaac
    31501 tttcgtagaa ataaaaatac atcaaaaagt aactactaaa ataagagtgg atgtaaaaga
    31561 aaaacagtaa taactagagg tgattaagat ggaagatatg acgagaattc ctcctcatag
    31621 tgtagaatca gaacaatcaa tattgggttc tatacttcta gataaagatg ctataataac
    31681 ggttacagaa actataaagc ctgatgattt ctataaagag gctcacaaaa taatatatga
    31741 atgtatgata actttaagta ataaaggtga gcctatagac ttgataacat taacagaaga
    31801 actaagaaaa caagggcatc ttaatgatat tggaggaatc agttatatta caagtctttc
    31861 tactatagta ccgacgactt caaatgtaaa atactatgct gatatagtaa aagaaaagtc
    31921 tgtacttaga aagcttataa aagcttctaa tgaaataata aatctaggat atagtggagc
    31981 aactaagatt gaagatgttt tagaacaagc tgaaaagagt atatttgata tatcacagga
    32041 gaaaactagt gatgatttta aatctataaa cttagttttg atggatgcct atgatatgat
    32101 agaaaaatta tacactaaca agagtgatgt cactggtata actactggtt ttaaagattt
    32161 aaacaaaaaa ataaatggtc ttcaaaggac tgatttaata ttaattgcag ctagaccagc
    32221 aatgggtaaa actgcctttt ctttaaactt ggttcaaaat gcagccttaa aaggtgatgc
    32281 ctctgtagct gtatttagtt tagagatgtc taaggaacaa ttagtacaac gtatgttatc
    32341 ttcacaatct agtgtagaat taaaaaaaat aaagactgga acacttaatg ataatgattg
    32401 gcctagaatt atagatgcta tggcagtatt atcagatgcc aaaatacaca tagatgatac
    32461 tcctggtata aaaatatcag aactaaggtc aaagtgtaga aagttaaaaa tagaaaaagg
    32521 actagattta gtgcttattg attatcttca acttatggaa ggtgaaggta ataatgaaag
    32581 cagacaacaa gagatatcta agatatcaag gtcactaaaa atattagcaa aagaattaaa
    32641 ctgtcctgta gtagcactat ctcagttatc acgtgcacca gaacaaagag cagaccatag
    32701 acctatgtta tctgatttaa gagagtctgg agcaatagaa caggatgcag atatagttat
    32761 gttcttatac agggacgaat attatcatgc tgattctgag agtaaaaata taggagaagt
    32821 aataatagca aaaaatagac acggtgaaac tggttctgtg gaacttgttt ggcttggtga
    32881 agttcaaaga tttggggata agttgagaga cctataatct aacgaatatc cacagaacgt
    32941 cctttctttc tacaggaaaa atgtggatac atgtcactaa atatgtccaa acattgaaat
    33001 ttagctaaaa aactatccac actatccaca gatggcctgt tgataagtgt gaataagtag
    33061 ataaagcaag cttttggctt gctttatctg tgtataaata ttaattatta ggaggaataa
    33121 tatggaaaaa ataagcttaa aatatatata tcccaatata attaaagtac ttgatgaaat
    33181 aaacttattt agagtaattg acaataattt aagagagtct attgttgttt atgctaataa
    33241 tgtggataat cagtatcata taaacatgac taatacaaac tttggaaaca taattaacat
    33301 atgtaaatta gaaaaacttt tagatgtgga taaatttatg gaaaaagtta taaaatatga
    33361 aaaagaaata atagagaaag aagagttttc aaaaatagaa gaatatatgt taaatattgg
    33421 agaatactaa aaaagttgaa aaaaatacga atgtttgata gaataagtaa gtaaagtgtt
    33481 cgagagtata taaaatatag aggtgaatta catgaaaaca gttgcaattg ttggttctca
    33541 atggggagat gaaggtaaag gtaaagttat agattatctt gctactcaag cagatgtagt
    33601 agtaagaggg caaggtggaa ataatgcagg acatacatta gttgtagaag gtaaaaaata
    33661 cgcattacat ctaattccat ctggagtttt aaatccaaat acagtgaata taatcggaaa
    33721 tggaatagtg tttgacccta aaggattttt agaagaattg gaaatgttta aaacggataa
    33781 tataagtaca gaaaatataa aaataagtga tagagcacat gtcatattcc catatcataa
    33841 agaactagat gcattgtcag aagaagccag aggcgattta aagataggta caacaaaaaa
    33901 aggtattgga ccttgttata tggataaaac agagagatct ggtattagaa tatgtgactt
    33961 aatggataaa gataaatttg ctataaaatt aaaagctcaa atagatgcta aaaatgaaat
    34021 agttaaaaat atatatggaa aagaagaatt atttgatttt gaaactatat ataacgaata
    34081 tctaggatat gcagaacaaa taagaaaata tgtggcagat acgtctgtta ttgtatatga
    34141 tgcagtaaga gcaggtaaaa aagtattgtt tgaaggagca caagggactt tattagattt
    34201 agatttagga acttatccat ttgttacatc ttctcatcca acatctggtg gatttgcaat
    34261 tggtgcaggt ataggaccaa atatgataaa agacgttgtt ggtatagtaa aagcatatac
    34321 tacaagagtt ggagaaggac cgtttgttac agagcaaata aatgaaactg gagataagat
    34381 aagagaacaa ggacatgaat ttggggtaac tactggtaga ccaagaagat gtgggtggtt
    34441 tgatgcagtt atagttaagt atgcagctag agttaatgga ttgactagta tttcatttat
    34501 gttacttgat gtattaactg gatttgataa gataaaagtt tgtacgtctt ataaaatggg
    34561 cgataagata ataacggatt tcccagcttc attagatgat ttagcaaaat gtgaacctgt
    34621 atatgaagaa ttagatggat ggaatgaaga tataactcaa atagataact ttgatgacct
    34681 tccagaaaat gcaaagaaat atgtagcaaa aatagaagaa ctagttggag taagtgtaga
    34741 tatggtttct gttggtccaa atagagctca aactataata agaagaaata tatttgctta
    34801 atattttatc aattaaataa tttaaaatag ttgaagaaga gtatctcaag ataatgttta
    34861 tagttattag agatgctctt ttatatttta cttacattga ttttttatta agttaaagct
    34921 acttatacat attctagctg aaattaaagt tgaatttatg tagatacata taggtttttt
    34981 aaaaagaatt ttgttgaaaa taaatggcta aagatgattt taattatatt ctaaaaaaag
    35041 taaaaaaaac tattgactaa aaattatata gacgctataa tataacttgt gagtgaaaga
    35101 aacgacgaaa aaagtcgaaa aaatgtggtc tattagctca gtcggtagag cacctgactt
    35161 ttaatcaggg tgtcccgcgt tcgagtcgcg gatagatcac caaaatatgg ctcgttggtc
    35221 aagaggttaa gacaccgccc tttcacggcg gtaacagggg ttcaattccc ctacgagtca
    35281 ccagtatggg actatagctc agctgggaga gcacctgcct tacaagcagg gggtcacagg
    35341 ttcgagccct gttagtccca ccaaattttg aattagagat aaatactaga ttatatgctg
    35401 gtgtggctca acggtagagc agctgacttg taatcagcag gttgtaggtt cgattcctat
    35461 caccagctcc acaaagtaca taaattcaaa ttaatataat tttctatgtt acaggatact
    35521 tttaagtatc tttttttaat ttcattttta ttgtattaaa aatgaaatta aacatacaac
    35581 aaaattttga attacttgca atgtgatagt tttaaatata aaatttagaa tcattctagc
    35641 atagtattat gtaatatagc aaatttaatt tatagtatca ataaatatag actactaaga
    35701 atgtaaatga tttgttagat taagatacat tagatatgta aatagaaaaa taaaaagtat
    35761 gttttaattt atgtgatata agttttaaac agagaagcaa gtttgctctt acctctctat
    35821 ttaaaaataa aaaaatgttt tttaagggct attttattta aatctttccc atctcaaatc
    35881 atctccagta aattctaaaa tcctaaactt atctaatatt ctagagagag ttctttgagt
    35941 gtaagtattt cctatttgag atggtgttaa gttggttgaa ataatgattt ttttaccagc
    36001 aacaagtctt gtatttacaa tattgaagat ttctccacta gtaaaagaat tattaagttc
    36061 agttccaagg tcatctatta ttaataaatc acaatcaaac aagtttttat aattatcttc
    36121 acttatttga ttatttgtgt ctcttctaaa tttataatct tcaagaatat ctaaaattct
    36181 aaaagaagtt tgatagatta caacattgcc tttatctaac aattcctttg caatacaatt
    36241 gcacatatag gtctttccta gtccagtaga accataaaac agtagatttt cactattatc
    36301 cttcttaaaa tcatgaacaa aattttcgca gatacttaaa ttatttagca tattttctct
    36361 aggagatatt tcaccatcag agcctttctt aggagaaaat atattcaagt taaaattcga
    36421 aaaattttcc tgacttaaaa tcctatctaa attagacatt ttataagctt catttacaat
    36481 ttcttgtttg aggcaagaac atttttctcc attaggtaaa aatcctttat ctttacaaga
    36541 aatacattga aactttattt ccaagtaatc aagaggtatg ttactttcag ctaaaagtct
    36601 ttctttcttt acttttaaag actctatatt ctctttagtc ttctttacaa tttcatcttt
    36661 agacttagga tttaaaagta ctatcttagc taaactgaga cctattttag aaatttcatc
    36721 atctatagat tttatctcag ggattctatt atatacctca ttttttctat gttctaataa
    36781 gagttcatta tcatctcttc tcttagcata tttagcaagt atttttctta ttttatcctc
    36841 attcatttaa tcacctactt aaacttttct ttttgacttt ttttaataat ctcatcgagc
    36901 tcatcagagg tatactgagt gaatgtttcg ttgaaattat gaaacttagt ttttttgtaa
    36961 gtattgttgt tttgagtagt attaatctgc ttcttattaa tattctcctt aactatacgt
    37021 tcttcctctt tttgctttaa atcatttaaa ttttttatat ttttttcatt ccaattttta
    37081 attataccat ttatataaga tatacttggg tttgaaatat tcttagactt agaacatgca
    37141 tttattatca aatctatatc catattaaat ttatcaaacc aagtgtccat aagttctttt
    37201 tctgattttg atggttgtct tgaaaatccc aattcattga aaatcgtttt atataaaata
    37261 tatcgttcac ttcttaccaa aaagctttct tcaacatctt ttggcgtata taaatttgaa
    37321 tcataccaat ttctaataat gccttcaatg tatttaactg gctttgatgt accagtttta
    37381 tctttgacgt attcatatgc gcaaagtatc atatcggggc tcatattgta tttattcatt
    37441 atatccataa tactcatctt ttcgcttggg tcgagatatc gcccaactat tttatttata
    37501 gaattaaaca tttttctaat acttggattt tcagaagtag atacaacctt atctgtatta
    37561 gattttatag atgaattatt gcttaatata ttctcaatat agaaattttt taaatctaaa
    37621 aattctattg aataattaaa attatcatat tctccattgt catgtatttt tataatcttc
    37681 ttttcttccc agaatttcca agcagataac acatcagaca atggtatatt taggtttttg
    37741 gctattgaat tattgtcgaa cttaggatta gaagtaatat cacatgcttg tctatatcct
    37801 aaaagataaa cttttacgta taacccgtcc gccattggca taaatatatc tataaagata
    37861 ttgggaatag tgatttcacc taaatctatt tcattgcttt ctttaaaaaa cattttatca
    37921 cctcagatta aattatatca ttttttatat tatatttgtc tgtaaacaaa taaaaggtca
    37981 caagattagc atgtaaatat tttctaaaag tattcatttt gaaaaggagg agatattttg
    38041 gttataaaat tcaaccaaaa agtaagaaga acattatcag tagcgattct atttatggta
    38101 ttaataatag gttgctttat agaaaaggat gacagtttag agacatacaa agaaaaaact
    38161 aattcaaaaa taaatcagta taatatggat gttatatttg atgatgagac taagaggtta
    38221 atgtgcaatc agaatgttga ctacataaat aatacaaaat caaacttaga taaaatatat
    38281 ttccatatat atccgaatgc tttttccaag aaagattttg ctccttttga aaaaagtgaa
    38341 atgacaaggg cttatcctaa tggatttaac gaaggatata tagatataaa gaatatatta
    38401 aataataata gtaaaatgga gtataaaatt aaaggagaca aaaatgacat attagaaata
    38461 gaattaggga aagagttaaa accaaatgaa agaatgtcat tagatataaa atataacgta
    38521 aaaataccaa attcaattgg aagatttgga tatggagaaa atactataaa tgttactaat
    38581 tggtttccta tagcttgtgt aaatgatgaa aggggatgga atttaaagag ttatgaaact
    38641 attggcgacc cattttatag tgagactagc gattttcatg tgaaactatt gattccaaat
    38701 aaatataaaa ttgctcatac aggaaaactt attgatgata aacatgataa taagaaaatg
    38761 ctatatgaaa tagaagcgga aaacgttaga gattttgcat ttatactaag tagcaaattt
    38821 aatgtaaaca aagtagattc aaagggagta gctatcaata catataattt aaataaaaag
    38881 ctttctaaaa aagcaactga agtggcaaaa gattcaataa atatatttag tgaattattt
    38941 ggaaagtatc cttacaaaga attttctgtt gtagcaagtg acttttttat aggggggatg
    39001 gaatacccta tgcttgttat gatagaccaa agtttatata atgaaaaaaa tgaattttta
    39061 ctagaatatg ttattgccca tgagacagca catcaatggt ggtattctgc tgttggaaat
    39121 gatgaaataa gtgaaccctg gttagatgaa gcacttactg aatattctac aatagtttat
    39181 tttgaacaaa agtatggtaa agatatggca aacaagctta taaaaactat ggagatacag
    39241 acaaaaagtt atttgagcga aaatatattt aagccagcaa atgagtataa aaattctact
    39301 gaatacagct tgaatgttta tacaaaaggc gcaatagcat ttaatgaaat aagaaaagaa
    39361 gttggagata aagtattctt tgagacttta aatgagtact ataataaata taagcataaa
    39421 aatgctaatg gaagagcctt tgtggaacta tggaatagta aaggtgtaga tataaataag
    39481 attataagtg aatgtaagta aacagtgaat gtaagtaaat tttgtgtaga gtattaaatt
    39541 ttatttaata ctctgttttt ttggtataat ataatttata aggcagaaaa agaggtgaag
    39601 gaatgctaaa gtattgttca gtagggagtg gtagtagtgg taattgccac tatatagggt
    39661 ataaaaatac taatatacta gtagatgcag gattaagtgg caagagaata actacaggtc
    39721 ttaaagacat tggtgtagat gcagataaat taaaaggaat atttataaca catgagcatg
    39781 ttgaccatat aaaaggagca ggtatattat caagaaagtt tgatatacca gtatttgcta
    39841 atataaaaac atggtgttct atgaaggaca aattaggtga catcaaagat aagaatatga
    39901 aagtatttga aaatgataaa acctactcat taggtgatat catagtaaga cctttttcaa
    39961 tagaacatga tgcgtctgac ccagttggat ataattttta tacagagagt gatgaaaaaa
    40021 tgtctatagc tactgatata ggatgtataa ctcaaaatat aaaaaaacat ctatataaat
    40081 ctaaattagt agtattggaa tcaaattatg accctaatat gcttatgatg ggttcatata
    40141 catatgcttt aaagaaaaga gtaatgtcaa gtactggtca tttatccaat gaagatgcag
    40201 ctaatttctg cgttgaactt attaatgaag gtacagaatc tatattatta gcacatttga
    40261 gtaaagaaaa taattttcct gagttagcgt atgagacttc aaaaggagta ttagcatcaa
    40321 atgatatagt agttggacaa gatgtaaaac tagatgtact atcacgaaat gatgtttcta
    40381 atgtttatga gatgaagtag aggtgaataa ctttgctaaa gagaaatttt gaaaatgttg
    40441 aggataataa aattttatac tctataaagc agggtatgat tttagcgata cctgctatta
    40501 tgactggttc aacggctctt gtaattttga atcttccaat aaaggcttat caagaatatt
    40561 tatcaggctt gtttaatgga gaggttacaa atattcttaa ctttataaat gattctactt
    40621 taggaattat ttcattaata atattattaa caataagcta tagttatgga aaaatatatg
    40681 ggagcaagta tacagtactt gttcctatag ttgcaatgtg ctctttttta gtattttcac
    40741 atggtaatga gcttaattct tatatagaaa tatttaaaac aaagtggtta tttacatcta
    40801 taattgtttc tatgacatct tctgtattgt ttgtaaaact tacagaatct tttgttacaa
    40861 gtgtaaagtt tcatacagaa ggggctgatg cagactttaa tatggtagtt tcggctatag
    40921 ttcctttcat aatagtagta tttttgtttt ctatttttag agtgataatg ataagtctaa
    40981 ttggcagttc aaactttcaa gatatatttt ataatttatt ttcaaatgtt tttaataaaa
    41041 tgggaacaaa ccttatgagt gctctgttgt ttattttttt aatgcacttt atgtggtttt
    41101 ttgggattca tggtagtaat gtattagata ccgtcgcaaa aaatttattt gaaaatagta
    41161 tggcaattaa cataaattta gttaataata atcaattacc aacagaaatt tttactaaga
    41221 ccttttttga tacaatggtt ttattgggag gatgtggctc tttactatgt ttagtaattg
    41281 caatttttct tagtgaaaaa cgtataaatg taagaaagtt agctaaaatt gcttcaatac
    41341 cagctctttt taatatcaat gagatgctag ttttcgggat accaattgtt tttaactata
    41401 ttatgtttgt accttttgta attactccta taatcttaac catcacaagt tatgtggcta
    41461 tgtctacagg tattgtacca tgtacagttt cagctgtaga atggacctca cctatatttt
    41521 taagtggata tatgtctaca ggttcaataa gaggtagttt gttgcaagta tttaatttat
    41581 gtgttggtgt tatgatttat attccgttta taaaaatgtc gcaaaataga tatacatata
    41641 tgtttaaaaa taatatagaa aatttaacaa acttattaaa aaggtatgaa ttaacaggtg
    41701 aacagcctaa tcttctaaac cataatggta aaataggttc aatttcaaaa atgttggcat
    41761 cagacttgaa gtttgctatg aaaagaggag aaatagagct tttttatcaa ccacaagtca
    41821 attataatgg aaatgttgtg ggagcagagg gattacttag atggaaacat tcaataggag
    41881 ggttcattta tccaccacta gttataattt tggctaaaga agaagggttc ttagatgagc
    41941 ttggagagta tattattaat aaagcatgta ttgatttaaa gaagagtgaa aagctattga
    42001 gaaatcctgt taagttctct gtaaatatat ctccagacca acttgatgac cctaagttac
    42061 cagagcaaat aaaaaatatc attagtagta atgatataaa tcctaatatg ttaggtatag
    42121 aaataacaga gcaaattgca ttatcaggtt cacaaataat tattgaacgt atcaatgcga
    42181 tacataactt gggaataaag ttaattatgg atgattttgg gatggggcat agctctctta
    42241 tatatcttca aaataataac tttgatatag ttaaattgga tggttcatta gttaaagaga
    42301 tacttacaaa taaaagaagt agtgaaatta taatgactat tgtaaatctt tcaaaaaatt
    42361 tagattttga tatagttgtt gagtatgttg aaaatataga ccaaagggat aagttatatg
    42421 gtttaggatg tgaaatatat caaggatatt attatagcaa agcaatacct tttgaagagt
    42481 ttgtagaata tgcaaatcaa gaaagagaga tataatttta taagagcttg taaattctag
    42541 taatgcatag atttgtaata aaattaagtt taggagaaat atatgaatat aagtatagta
    42601 gtagtaggta aaattaaaga aaaatattta aaattaggta ttgatgagtt caagaaaagg
    42661 ctttctaagt attgtaagtt agaaattata gagttggatg atgaaaaagc tccagagaat
    42721 ttgagtgtta aggaaatgga aattataaaa gacaaagaag gaaagaaaat tttagggaag
    42781 ataaagcata atagttatgt tatagccttg gctattgatg gtaagggttt atcctctgaa
    42841 gaacttgcta agactatgag tgatttagca gttagaggta atagtagtct ctgttttata
    42901 ataggaggtt ctctagggct ttctgatgag gttttaggaa gggctgatta taggttatct
    42961 ttttctagga tgacttttcc tcatcagatg atgagattga tattgttaga acagatttat
    43021 agggcttata ggataaataa tggagaaccg tatcataaat aaatgatacg gttttttatg
    43081 atataattga attataaaat aaaatcaaaa tgtaaagtag gtgtagtaat ggacagacta
    43141 gatatattta tcgaaaatta tgattttaaa gaatatcttg aaaaattacc tagagaatta
    43201 atagaagaaa attataaaga ttttgttata gaattttgga aaaatcctag tgtagatatt
    43261 attaaactaa aatcaaaata ta
//
LOCUS       DAELLV010000009        40797 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.009, whole genome shotgun sequence.
ACCESSION   DAELLV010000009 DAELLV010000000
VERSION     DAELLV010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 40797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40797
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.009"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            152..607
                     /locus_tag="KPD58_000443"
     CDS             152..607
                     /locus_tag="KPD58_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264230.1"
                     /translation="MGLRDKFAQSFARSKTMSGPEKKANEIMGKLLLKKAILPIVLMF
                     VIIIAGAMLKINSWVTLGINLVIAVGAFFYIRNSSKKYQNFKPYVGNLISLEKKGKKE
                     YVAIIKQGKLPVKLQIAYGGEDLEHVKKNQMVQISYNPDAKIAILVNRQ"
     gene            complement(828..1088)
                     /locus_tag="KPD58_000444"
     CDS             complement(828..1088)
                     /locus_tag="KPD58_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264231.1"
                     /translation="MDKLITELSSLGAIGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVQMGIMKTDVEEIKEDVTGIKGMLQNGV"
     gene            complement(1351..1704)
                     /locus_tag="KPD58_000445"
     CDS             complement(1351..1704)
                     /locus_tag="KPD58_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264232.1"
                     /translation="MFKFDKNKIEQIKQGRKVEMQYKDISDISIGQVKQDDDITNNFI
                     ANAEIYEMLLNQSSVNEASNISSFSVRKSGGESGMVEVYVALILRGKKTIEEVPAVIR
                     EQVRIRCKELEIPVE"
     gene            complement(1721..7042)
                     /locus_tag="KPD58_000446"
     CDS             complement(1721..7042)
                     /locus_tag="KPD58_000446"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012632.1,HMM:NF024930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264233.1"
                     /translation="MKRTKLLQRGNFFGDKNMVVDEFDEGYDNYDFINFFTGCCNYTF
                     GLKNNNILYGCGDNSNFQLGLGEDNTTRKLFTKIPNISTNIKKVACGESHAVILTSDG
                     ELLVAGINTDGQMGLGLEKVGKTVSTFEKVPEIKGVKDIACGLQSTYLLYNDGTLYVA
                     GNNLYGQLGLGTNGASANVNTFTKVDVDNVKAVFSYNKSAFIIKNDNKCYSTGFNNQG
                     QLGLGDKNNRDLFSLVSINDVKTIACGSEHTVLMTYNNDIYGCGKEKCFGNALQSSLF
                     TKIEEVNIKTIACGHGNTMLIDNKGTLKVAGNNDIYQLGIANYSENIDNSFIDLKNIV
                     AKNIFIGLSHSILIDSNNDSYCTGDNTYGQLGSFFDDMHIVEFKKMDSEKYSYSNYIN
                     LIKSEDKLTLLKEEMEIKDIELPLDIHSVRDVVFSPYCTLVILGNGDVYGLGNNRYKG
                     MGSDLPSQLNELTKLSISNVKSIVASKNISGGIFYIKNDDTCYYSGPNSNSIAGVLPS
                     NSDIFKKISIDNVKKVVINTDLSNWFSLIVTNNKQIYTSGKSSSYVNGLSNKSASQYT
                     EIGVSNVTDAYSSYNATFIVADEKKVYATGINTNYLLGVNPNDGVNINLTLINNWFYV
                     NPSGASYSTNSCTNDITKINNIIIYEYMAILCTNYGSFLTGYHGTLWTKPSDSTYRVQ
                     QQGITHAGYISNYIYNYYPTRCTQSSYSTNFAYLYYGESTSNLKNVNPDNLLISGGSS
                     YIHQYGRNYLNNQSSNNIAASNINSGPITSDKAIFLYKALLYLSSNTLYGFGNISESA
                     KELDVSDTQDGYNATNYKKVMKNIKNIFIPPYDLSRDKTRFAILTDKSLFICGYNSKG
                     THGISVNSSLNLNNKINYNKKNSSSEISSNIQEIYSHSKSTYLLTNNNMLYSVGLNDV
                     GQLGVGDEINRKVFTKINIDNIKSINVNRFTDNSKHVFAIKNDNTCYAVGLNNSGQLG
                     IGDNVNRNVFTKINVENVKYVAVYGNTSLLLTNDGLLYGAGNNGKGQLGLGDTTSRNI
                     FTRIPINGVRDVYICNDVSIIVKNDNTCYVCGFVNGYFGFTEGSISTFTKINIENVKS
                     VVTAGSEATFFITNDNVIYTTGKKERVFFSTETNDIKGIRVINNIINAKKIVVNGYTS
                     AILTNDNKLFVGGLSGYGSIANNNNTNSVEDVKDVFVTANNTLYIDNNNNLISSGRDT
                     YGISDESYRDMSVPYYKVSIKKDVDTVFSSYNTIFIKDIYGKFYSSTGDNRYNHLGIH
                     HRYDNDKNEALEGSLHSYFKTDNTSDKIVFNKKNEKLVMFNDKYIKTNNKYINYKNIF
                     KDNFKYTSIILPFEVSDIDISKTHSLAVAKDGKLYGIGSNSYKEINQTLEDIELLTLT
                     EVNISDVKKVACGDNYSYIIKTDNTLWSYGKNTEYQLGVGHNNDVRELQKVTGLPSVK
                     DISIYNSMTLVLTNEGELYAQGYNTNGLFGLGESEKDKIIRTFTKVLTNVKEIKSHND
                     DHILVIKNDNSLWITGKNKSMYKISISITDLYEFTKIPIPEHLNDILDIELSDDTIYM
                     ITKVDTSKASIEIVEKSISQVRVVVQDPNNVIEKLEMFINDELISTKTNLEINSIIFE
                     IPQNKIVLGENKILIKASSPTGDLYSSMFIFKSETGLKVKKDSILMINNKVYSIINIT
                     ENNTDLIVTLNEGLKDDMMENNPIYQLINKTKVQVKINKSDLFKDMKLVEIKKSDSSY
                     QEIYELEEANIKSAQPKIIVEKGDKWTAIKRPSMIFRYDAESNEPQA"
     gene            complement(7059..8042)
                     /locus_tag="KPD58_000447"
     CDS             complement(7059..8042)
                     /locus_tag="KPD58_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBG0264234.1"
                     /translation="MEEKFYIILTKIGREKIANATALGELVGLTKFQVGDSNGEYYEP
                     TEEQTALKNVVWEGNINSLRIDEKNPNWIVIETILPGTVGGFMIREAAVLDNENNIIA
                     IGKYPETYKPRAEDGSIKDLVVKMILQLSNTSNVTLEVDPTLVFVTQKDIQDLDDKFD
                     KNIKEIKVKIGEELLSTEAKNLSGAINEVVEKIKNISIDDVIGGQIQTELSVLKNSYN
                     KLSEKVLDILIYLELESEIDVDEAGYWYDTLTNAKNIIAIEGLKLDLNRKCITGELGS
                     VTFKNVVLPFNANRVRYIHEMDNNFVETKSNRAYSIGQTDITLNKYSYEIR"
     gene            complement(8043..8741)
                     /locus_tag="KPD58_000448"
     CDS             complement(8043..8741)
                     /locus_tag="KPD58_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HBG0264235.1"
                     /translation="MKLIDKLPSFDRNYIVEEIQGAYDTELNILKEDIDDTFNQLFVD
                     TATWGLDMWEDILCIEKKELDFDTRRSNIKAKMRSRGTSTIEVIKSICEAYTKSETDI
                     KVYSDEFTFVLSFIANNCDYKTLLDCSDMIERVKPAHLLHYLEPIILDKSMVYCGGGM
                     VCSEEVKVHPYFEPIIKCSAVVNCGAGMISREEIKVYPLSIKCIENNCKINIAIANDT
                     GVENVVVYPKSEVV"
     gene            complement(8738..9790)
                     /locus_tag="KPD58_000449"
     CDS             complement(8738..9790)
                     /locus_tag="KPD58_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBG0264236.1"
                     /translation="MYSDQTYEVIKNRTLENINLDIYKGEGSFLNNMVSGNNLELSKI
                     YLELSKMHKMAFIQDTYNQFLDKRVNEFGVYRKLGTESNGEVEFIGEKGTVINNGTII
                     SYRDLLFVVIKDVTIGSEEGDNSPVQALEVGKKYNLPTNCEFKLVDNISGVTKITNTR
                     SFEGGTDIETDEELKERFYKIQRNQATSGNKAHYEEWALEVDGVYNVKVYPRWDGPGT
                     VKVLIFGKNNQAVDTETIERCQQHIDEEKPIGPTITVVTPLPIEISISAVMKLEDGYT
                     LDNVKESFLESINTYFRDIRGEIIYTKVMGILINTTGVHDLSNLLINGSTDNITINED
                     KIPSVTTVNFSEVENQ"
     gene            complement(9783..10211)
                     /locus_tag="KPD58_000450"
     CDS             complement(9783..10211)
                     /locus_tag="KPD58_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBG0264237.1"
                     /translation="MSTIFPFIGVPEDYILPKTEELPIFREVAWDFEKDEPILEKGDF
                     KIIEKKEALKVWIYKCIKTNRYEHEIYSLEYGTELSELIGQKYTKGLTESEASRFIKE
                     ALLINPYILEVNVKSANFNRDILSANVKVSTIYGEVEINV"
     gene            complement(10211..10537)
                     /locus_tag="KPD58_000451"
     CDS             complement(10211..10537)
                     /locus_tag="KPD58_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HBG0264238.1"
                     /translation="MANPINEFIGIIREEGKYHNQPSFFIGKIKSKLPDLKIETNNII
                     LEKEDILIDSWMIDRQLETFDTETNQEHQHEVKNPFIDNFESGDMVIMFRIGEKFAVV
                     SKLVSL"
     gene            complement(10553..12082)
                     /locus_tag="KPD58_000452"
     CDS             complement(10553..12082)
                     /locus_tag="KPD58_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG0264239.1"
                     /translation="MIINRSKDSSSNEISFVSKDMGFLLTQSEVSYNFKDKLVEDIAK
                     QVFAENRLSVGIIAKTNVKYTKMFIGVNGYDTIMSAYTEASKKTKKKYMIEANLDKFN
                     VIEKGTVTLSVMFEEGFNIINTTFSESMENVKNKVIVVDQYGSKISEKIDNEIFKEVN
                     VIMQKVIQQQENQDVDIDSEFNGIEKSCSLKGYGDVSCITGRGVKVKDSYTKLVGLFY
                     IDTDKHTWQNGEYQIELELNFQNLMDEKSAGQDEPKEESNLGGEDYAGGKEFTAEFTA
                     YCPRKEEGGDTDCRKKKLDPSKKTCAAPMVGKYEQTYYTKEFLNKHPLLNYGDEIQVI
                     TGVSGRDGVYKVNDVGPAITIEKNGTYHIDILFGNVEEASKFGRRKGKIIIGGYSGNV
                     SDKAKIVISEAKKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVSLPRTSNQQSKKGK
                     KVEQKNLQAGDLVFFHNPVSHVGLYIGNGEFLHAPQKGDVVKISKLSSRRDFNTARRV
                     L"
     gene            complement(12364..12786)
                     /locus_tag="KPD58_000453"
     CDS             complement(12364..12786)
                     /locus_tag="KPD58_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264240.1"
                     /translation="MEMWLRQAEDRFRFPVFPSSFSINGKAAVNSSSILKIGEIATFG
                     GVALKSISISSFFPNKDYTFCDYTGFPSPYDCVNKIEKWMKEGFILRFTITETNINME
                     VIIEGFSYEERDGTRDVYFTLDLKEYKRIKIPKVTPKQ"
     gene            complement(12786..15239)
                     /locus_tag="KPD58_000454"
     CDS             complement(12786..15239)
                     /locus_tag="KPD58_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264241.1"
                     /translation="MGNVREEGINMYLTDNYTPKMNQIISVTDNFRRATVAVSLSTNV
                     MASSIKNSIGSASNRVNSLNSSLRKVQTTASSVSSTMTKLSSSINAVSGVIGSLNGSI
                     MRLAITIAMIIDYFNKLIQKKNEFNSNIMIILIFKAKSDEVEKTKNKLLGNLKKIGGK
                     IWNIVIKAKDMTKRVISSILGKLKQVEKRPYQGSINLKDMVSSAMGRILPKLMLFKNT
                     FWSGVIAIKDMASGIISKVFPKLRLFAGKVWSGAIAVKDMASGILGSIKGKISDLTNG
                     ATIGVAVKKGVDLLGQEQNQKVVLESVMKRNTGKVNQIDVDDYYGSLVRMANDTPFDP
                     EDVVAMGTKAKMISNITGGKKEKDITQAMVDVRALNMNTSSEQDVSAAFLSAAKGNME
                     SLNTLVGENYKTFDEALEGISVKQMGLAKEMSNTIPGIISGAQTSINNGLKSIVKPFD
                     DILGQGLKKIKTFIESGLGNLAGLSEKMAGKIGNVMNGKIIIGNKYDQMQSRSVKNGK
                     EFSDSTQYRISNEAEKRKMMVENKQERFENHAATMIGNAPKAIVNAGSTLLQNIDFTA
                     LIDSLLPVVNLVNNLLDSINNKSPIAQGLISIFGTIVTTAFQLIGPVVEAVSPIITRI
                     FTFLGEYAPQINNFIETLGVIWKTVWETLGPLLETGWKIIEPILGAFFNILDKVCKIV
                     KDICKWWQTMINKIKNGSITGTVLNLVEKSKKNYKDNPYAGTKAGDSGKAYSGKKGNN
                     AFGLNYVPYNDYQTRLHEGEMVLTKQEANQYRSRKNGGNINIAKLADTIVIREEADIE
                     KITSKLVASIQLAQLGGVL"
     gene            complement(15239..15406)
                     /locus_tag="KPD58_000455"
     CDS             complement(15239..15406)
                     /locus_tag="KPD58_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264242.1"
                     /translation="MYYYWKKKGIRPSLFYAMDKGELKLIEAFFALEIEEEVEKMKHG
                     YGVCPLTGGGM"
     gene            complement(15433..15879)
                     /locus_tag="KPD58_000456"
     CDS             complement(15433..15879)
                     /locus_tag="KPD58_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG0264243.1"
                     /translation="MSENGLSKNINIVDLLLNSDTENLERPSTIVELKRLSTIFGQEF
                     KVMCRALTISKDEEIQNTCLKIDENMKTDIDLPEMQMLTIIEGVCDLDGKLLFKNKEL
                     MDKFKAPTPKELARKLLLPGEITNLYRILQDVMGYGKNAVIEEVKN"
     gene            complement(15993..16421)
                     /locus_tag="KPD58_000457"
     CDS             complement(15993..16421)
                     /locus_tag="KPD58_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBG0264244.1"
                     /translation="MANMEARNVMSGTWGELWLDGNKVAEVKKFQAKMEFTKEDIIIA
                     GQMGTDTKYMGYKGKGSITLYHVSSRMHKLIGEKIKRGSEPRFVAISKLNDPDSYGAE
                     RIAVKNIAFDDLTLADWEVGVKGEIEAPFTFTEYDFLDII"
     gene            complement(16435..17499)
                     /locus_tag="KPD58_000458"
     CDS             complement(16435..17499)
                     /locus_tag="KPD58_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HBG0264245.1"
                     /translation="MAIGLPSINISFKELATTVKERSARGIIAMVLKDAKALGLNEIH
                     EKEDIPVDLSAENKEYINLALMGNVNTPNKLLVYVIEGEADIQTALDFLETKEFNYLC
                     MPKAVEADKTAIKNWIIKLRDIDKVKVKAVLGKVVGNHEGIINFTTEDVLVGEKKYSV
                     DEFTSRVAGLIAGTPLSQSVTYTKLSDVVDIPKMTKVDAESRVNKGELILIKEAGAIR
                     IARGVNSLTELTAEKGEMFQKIKIVDTLDIIHSDIRKVIIDDYIGKVTNSYDNKCLLI
                     VAIKSYLEELEKSALIESDSTVEIDFEAQKSYLKSKGVDLSYMTLQEIKEANTGSKVF
                     LKAKIKVLDAMEDIDLSIEI"
     gene            complement(17504..17947)
                     /locus_tag="KPD58_000459"
     CDS             complement(17504..17947)
                     /locus_tag="KPD58_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264246.1"
                     /translation="MLKYKEILETIIEILKKNFTESIFIDDESVQGSEGSCFFVSILS
                     VICTPVMLNTNNKDIVISIKYLPKPQSKSIRMYEISDELNKLFNRNIKVTDRKLNITK
                     LEQSIKKEESIYVLNFTFTLNYLDSVYEEDVVYENMKEINLNLGE"
     gene            complement(18196..18636)
                     /locus_tag="KPD58_000460"
     CDS             complement(18196..18636)
                     /locus_tag="KPD58_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264247.1"
                     /translation="MENKKDILFKETDKRLHNYKYLDIKIKNINLDIKRCENEYSGCG
                     AMVYTEKTSNTYNISSSVENEVLKREERLRKLKMEKEDIEIEKEKIENALTCLNDIEM
                     EFFNLFYNSKTKNNMTYISMKLHLDRTSCYNLKKKMIFKLSEIL"
     gene            complement(18741..18938)
                     /locus_tag="KPD58_000461"
     CDS             complement(18741..18938)
                     /locus_tag="KPD58_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0264248.1"
                     /translation="MFKNNLKYYRKCKGMTQIQLARKAGITNDYISQIERGIKNPGLL
                     MAKKISSILEQNIEEVFFIQL"
     gene            19107..19427
                     /locus_tag="KPD58_000462"
     CDS             19107..19427
                     /locus_tag="KPD58_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBG0264249.1"
                     /translation="MFAKRLRELRKEFGLTQRELGEKVGVSQRVLGYYETENRFPDEH
                     ILNKLADVFNVSVDYLLGRTLVKENIDTVAAHRKNPHEELPEEAQEQLNDYIEFLLNK
                     YKKK"
     gene            19509..20015
                     /locus_tag="KPD58_000463"
     CDS             19509..20015
                     /locus_tag="KPD58_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264250.1"
                     /translation="MNNLDKLFELASQEEIIIHYTTYIAGDLEGLYINKHGIKIISLL
                     SNLKQNSKKLTSILAEELGHHFTSLGYYVSSYNDYYTKIIIDKCENKALKWACEFLIT
                     EEDIINIINSGITCVYEMADILNVDITFFQKRLEFLSLKKQSLQLGNNKYLILTNLPY
                     FYIFDPIS"
     gene            20716..21471
                     /locus_tag="KPD58_000464"
     CDS             20716..21471
                     /locus_tag="KPD58_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG0264251.1"
                     /translation="MEKKVLATVGEKEITNIDIENALKSLDPYQAMQFKTEEGKKHLL
                     NDLVNQELFFLDAKEEKLDEEEIFKLEMKKIEENVLKQFAINKVLSSVNVTEDEKVKF
                     FEANKSSFSKPESATAKHILVDSDEKAKEILAQIKSEEISFEDAALKHSSCPSKDMGG
                     DLGTFGRGQMVPEFEEAVFSMAKGEVSEPVKTQFGYHIIKLEDLQESTESTFDEVKAE
                     VEKSLLYQKQNEVYGNKINALNAKYGNLVKYND"
     gene            complement(21614..22042)
                     /locus_tag="KPD58_000465"
     CDS             complement(21614..22042)
                     /locus_tag="KPD58_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBG0264252.1"
                     /translation="MNILFFLTPKSEVAYIYEDYTIRQALEKMEYHKYSAIPIISKDG
                     KYVGTITEGDFLWTLKNDLNLDLKGLEDVPVTDINRKMDNSPVSINADIEDLVIKSLN
                     QNFIPVIDDQDTFIGIIKRRDVIGYCYEIIRGYKNLANDN"
     gene            complement(22463..22663)
                     /locus_tag="KPD58_000466"
     CDS             complement(22463..22663)
                     /locus_tag="KPD58_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBG0264253.1"
                     /translation="MKTGVVKWFNNEKGFGFISVEGEGDVFVHFSAITGDGYKTLEEG
                     QSVEFEVVDGAKGPQAANVVRL"
     gene            complement(22846..24015)
                     /locus_tag="KPD58_000467"
     CDS             complement(22846..24015)
                     /locus_tag="KPD58_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HBG0264254.1"
                     /translation="MGGVALKILKNKNKYIKGIMAFVFLSLATTAGVFLINDLSMKNM
                     EEKVMESANVEGDDVKDQYKLLLENLFDYRNKAILEKNENILKGLYETDKKFGLWAYE
                     NEVKKMKYLENWSSKQGVKFKDIKTRVKIKKIKEKEKDLYGIICSVSTEYKYSYENQK
                     DKENAFRIGTYHYLNVKIIDNQYVITKEWYTDPFADSLNLENIKSDDIRNYIGQQDGV
                     ELQLTDEQKKAIDYAHKYCGAAADEEHGMKFNPNYRDYNPEGGDCANFASQIMFESGR
                     FKKNSIWNYSERAGTKAWVNAQAFKNYALYSGRGSLIAKGSYEEVYKEAYNLRPGDFV
                     GYEKKGRITHVSTVTGLDSRGYPLVTCHNTDRMLVPWDLGWSDKSIRFHIIKINY"
     gene            24251..25072
                     /locus_tag="KPD58_000468"
     CDS             24251..25072
                     /locus_tag="KPD58_000468"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HBG0264255.1"
                     /translation="MQKKVAAINDLSGIGKCSLSVAIPILSALKVQCCPFPTAILSSQ
                     TGYPEFTFLDFTDEMVKYSNVWKNLKVNFDSIYSGFLGSKHQIEIVANFINDYPNAFI
                     VVDPVMGDNGVMYPIFTEEMRQEIKELVKHSDLTTPNLTEACFLTGNDYTKSDYNRDE
                     LIYIAKSVSDLGPSKVVITGILEDDNILNLAYNRDNDHVFFTSVKYNNCSYSGTGDIF
                     TSILCGMLVNKHDLGVAVNTATDFIYKTINYTSQFDTDRNDGVMFENFLSDLTNI"
     gene            complement(25148..26536)
                     /locus_tag="KPD58_000469"
     CDS             complement(25148..26536)
                     /locus_tag="KPD58_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase"
                     /protein_id="HBG0264256.1"
                     /translation="MLGGKKSNNKKVEKLRTIFIKYLSLFFIMTISIVLLLMLSFSVL
                     LSSGVILPANYAEKQFNKYKEQIISSEKVTEDIIPSIYEYGVYTLDGNLISGTFNKKE
                     SKEVWNLMRDIEERHAYSESYIKFFKKDEVFIIKYKIVSEYSSPILRAYLPKPETLGM
                     IIFSIIFFIEIVILSKVFGKKFNIEMELLKNTTEKIEQQDLDFVVESSKIYEINNVLF
                     SMDKMKLALKDSLEKQWMLEENRKEQISALAHDIKTPLTIIHGNTDLLIEINENPELL
                     EYMEYISKGVTQIEKYINTLIDISKTENGYILNKEAISMSEFIKDVLVQIEALASTKN
                     LDVEFYKQDNLPKNITIDRELLFRAIMNIISNAIDYSPSQSKLYISVSVHNKYLKFVV
                     TDCGSGFSKADLSKATEQFYTGDLSRNSKSHYGMGLYIVNNIVQKHNGTLCIENSVKT
                     GGAMVTIEIPIV"
     gene            complement(26671..27429)
                     /locus_tag="KPD58_000470"
     CDS             complement(26671..27429)
                     /locus_tag="KPD58_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HBG0264257.1"
                     /translation="MRQLIRIFCSDLIKLKRNFIILMHICIALMGMGLFLWYYKVSGA
                     DNILKIVAYLQVIAIAFPLLSSVVCSMFVEQEYYAGNYKHMLTTSNPKYLTLISKYII
                     LVCLGFVATLVSVLGFKFGISKAYFTSSFYMISILILIGCNLFEYILHLFLSLRFGKG
                     TSIGVGIVEMLLSALLLTGLGSGIWQYFPCAWGVRFITIWSSFSSSKTVEYIKVESIK
                     AYQSTGLICGFVTILAFVILCIWFSKWEGKKSEE"
     gene            complement(27431..28147)
                     /locus_tag="KPD58_000471"
     CDS             complement(27431..28147)
                     /locus_tag="KPD58_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HBG0264258.1"
                     /translation="MERFIRVEFQKMKGTFGMKLIWIVPVFCILLCGLGGGQNAAFNW
                     WYTTFLPGALTLISSLIVLKDEKSKYRGILPLPIEMKDVWTGKMIYCIICCFIASIIS
                     AIGVVFVGIIYKETISIGQNIIASILIILLFMWQIPLCMFLASKFGMFVTVLTNMFFT
                     IVGVAVFSTTEMWYMFPYSIPSRLMAVVLHILPNGLPVPEGSEFLNNAVILPGVLISI
                     VLFVLFALATRVWFNNREVK"
     gene            complement(28149..28856)
                     /locus_tag="KPD58_000472"
     CDS             complement(28149..28856)
                     /locus_tag="KPD58_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HBG0264259.1"
                     /translation="MSNYILQVKGVSKKFKEQMAVDNISLAIKSNSIYGLLGPNGAGK
                     STLLKMITGILNPTSGKIMFEGHRWTRKDLSNIGSLIESPAIYENLTARENLKVHTTL
                     LGLPDSRIEEVLETVDLRDTFKKRSGQFSLGMKQRLGIAIALLNHPKLLILDEPTNGL
                     DPIGIEELRELIRSFPKQGITVILSSHILSEVEQVVDEIGIISNGVMGYQGEVSKEQN
                     LEELFMKVVAENRKRGQ"
     gene            complement(28972..29508)
                     /locus_tag="KPD58_000473"
     CDS             complement(28972..29508)
                     /locus_tag="KPD58_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG0264260.1"
                     /translation="MNKIAVSFLIIATTLLSTACMDYSISAVELVDSKESAVVKKDED
                     AKEETTSKMINSKKTTKIPIEIISKDEKIVKYLQIDEESSLKDKLRLILDTLSNEYFN
                     GLDMEVEVKEKDNLVKINLIEPDKKSRVSWKDDYLNEQNIIYTINNIIKNVIQEEDNS
                     IWIEEVEIYYNGKLIELR"
     gene            complement(29610..30680)
                     /locus_tag="KPD58_000474"
     CDS             complement(29610..30680)
                     /locus_tag="KPD58_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBG0264261.1"
                     /translation="MNKKLAIFAAVLLVIGIIGTTWSGILVMPSLINFGLEKEAELNK
                     ENKLYQEKVNIDKLDIAVDNINVMIRKSSNEDVRVTTRGNNEFYKYSVTLQDKTLVVK
                     GERKSENKIKKIRSFDHLLNTSINSMFSRDYREIRIYVPNNVDINASSISSYLFISDD
                     VDSNNITYKTSSGGFSRAITENKVNRLENLNLISNSNLHLSTKSILGVKNVNIESESL
                     DISSENGDVFINNIEEYIPENVNIKEKIGRNSNYESEFYLYSDMPIAKFLDIEVPNSK
                     VRLDIPVNKYKFNCDIKSKEAIEEFNNDEEYDNDSYDYEHNEEHSNKYRNTREIKGLL
                     NKNLSNLEKEYTIKINSNSLEL"
     gene            complement(30673..31731)
                     /locus_tag="KPD58_000475"
     CDS             complement(30673..31731)
                     /locus_tag="KPD58_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HBG0264262.1"
                     /translation="MNLNKTEFLEILKDYLKKDFSESEVNDILRDYEEYFVDGLIEGK
                     SDMEIIASLGSPKSIAKELILQTKEKNIEEEKIINTDVLNDKFSKFKSDMKKGYSILK
                     DEAKEKYSKSKENIDGKIMNTDILNGKFSRFKSNVKKDYSILKDEAKEKYNKSKENIN
                     DKLTPSIEEGVGGLSRQVIKGLLVVLSLFLIVPAFAFVCAIVSATIALVCSLIAFVIL
                     IPFVIKFIAMVPDVMMFCIFATIAFVGLEILAWQILLFAIKLGKQLLNNYIHWIKRKN
                     IYIKANEKMEKRKKATLNDEDLNKDINENSYNNDYYNREEESIKETDEYNDDSINFEA
                     IYKDLEDIENKKGEDKHE"
     gene            complement(31718..32050)
                     /locus_tag="KPD58_000476"
     CDS             complement(31718..32050)
                     /locus_tag="KPD58_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBG0264263.1"
                     /translation="MNTQFRKGVLEICVLALISKKDMYGYEIVHNISKVIDVNEGTIY
                     PILRRLTKDLYFETYILESNEGPARKYYRITSLGKENLSNLMEEWREFVKAVEILISE
                     DEGGNELE"
     gene            32249..33370
                     /locus_tag="KPD58_000477"
     CDS             32249..33370
                     /locus_tag="KPD58_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HBG0264264.1"
                     /translation="MKDDKTILILTAQFGAGHISAAKAVKECIIEKYSNYNVVIQNFI
                     NASIPMMNKPMVKLYENNTKYTPGLYNYYYYFKKSFDSRHDLSHKLYTPKLSEYIADI
                     NPDLIISTFPLAAACVNNFKIKNPDINIPTLTVITDVVDSMEWVFENTDLYFVPSPEI
                     KNRFFQKGINPDSIKVTGVPVDKRFQIESKELCCDKYRLLLLGGGRGLFDIDEDFMHW
                     IDEFIEEHSNSIEITIVTGKNKKLYDNLTHKKPLKNIKVLGFVNDMYNLIRECDLMLT
                     KPGGATIFEAIQSQTPVLVKMPKVGQEIENAKFIIDKGLGMIYSDDLDLKNIFYKLVS
                     NEFDSIINFMKKNLKEFKTVIHPEKIADYISELIDNHYS"
     gene            complement(33498..35396)
                     /locus_tag="KPD58_000478"
     CDS             complement(33498..35396)
                     /locus_tag="KPD58_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HBG0264265.1"
                     /translation="MDSLLYVIEKDKHTNEELREILKSNKNIRFVSLMGVDLGGNATD
                     EKIPVELFLEDIDKFLESAIQTDGSSVELYNIATLNNAKVDLMPDKSCHWYVDYNMEY
                     IDEEVGLPVGTLKIPAFLIHDNKKVCSRGVLQKADKYFKKSMYEIFREYPHVINNIGI
                     DSVDDIEEIMLTAATELEFWVNTPEDKADLEKLYVSQSLKEQYWKRTHGIIRTCLEKS
                     LIILQKLGVNPEMAHKEVGGIQSSISIDGRTNHAMEQLEVSWKFSTPLQAADNELLVR
                     DVIEDVFTSHGLEVTFKAKPIHGVAGSGGHTHVGVSAKLKDGSIKNLFAPKDLKEDYL
                     SELGYGALMGLLYNYEVLNPIVTASNDGFNRLVPGFEAPVCIVTSLGHSYEIPSRNRS
                     VLVGLIRDMKNPKTVRFELRSPSPLSNTYLVIAGCYQTMLDGIKAAAKSGLSTKELEK
                     ELSKNVGEESFYLEKDRAYRDENDVFEHYSLEERNARFGIPPATVYENMKNLEIYASK
                     LKSLKQGDVFTDSIIESFKIGAIDKWQKKLKTRIIEAGIQKIRSIVKIHTKENMDALD
                     EVVWNSISDLKFNIMKDTLTRESLFTRVREAIENKDYQTASDLQIELKRSMEEIQQLY
                     MQYKKNIY"
     gene            35711..36487
                     /locus_tag="KPD58_000479"
     CDS             35711..36487
                     /locus_tag="KPD58_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBG0264266.1"
                     /translation="MEYIIFDLEFNQGFDKKLNKTVSNEKCPFEIIQIGAIKLDSKFN
                     IIDTFNSYVKPTIYKDIHPFISRMTNIKSSDFNDSPTFPEVYNNFIKFISSQDPILCV
                     WGAGDLKELYRNINYHKLPSNSLPKSYINIQQHASKYFNNPAGKSIGLQNAISILELD
                     EKMSYHNALNDAYYTAKVFIKIYNPSIVPDIYLYTSIKPKTIRYSNKKRVDYDKLFDE
                     FRKILNRELTKDEKKIINLAYNMWKTNQFTLENVKQRKNK"
     gene            complement(36579..37331)
                     /gene="xth"
                     /locus_tag="KPD58_000480"
     CDS             complement(36579..37331)
                     /gene="xth"
                     /locus_tag="KPD58_000480"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBG0264267.1"
                     /translation="MKFISWNVNGIRACVGKGFLDFFKEVDADIFCLQETKLQEGQIE
                     LDLPGYFQYWNYAERKGYSGTAIFTKKEPLKVMYGINIEEHDKEGRVITLEFEDFYFV
                     TVYTPNSQSELKRLEYRTRWEDDFIDYLTKLDNHKPVIVCGDMNVAHKEIDLKNPKNN
                     MKNAGFTKEEREKFSKLLDSGFIDTYRYFNPDKEGVYSWWSYRFNARKNNAGWRIDYF
                     CASKKLEDRLVSADIHTEILGSDHCPVELEIK"
     gene            complement(37492..38442)
                     /gene="galT"
                     /locus_tag="KPD58_000481"
     CDS             complement(37492..38442)
                     /gene="galT"
                     /locus_tag="KPD58_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HBG0264268.1"
                     /translation="MKELRIDPITNDVVIFATDRLKRPLDTADIPNEDEETNEYDEEC
                     PFCKGNEAHATDALFEIDSEEGWLVKSVYNKFPIIDDMARDVYGVHEVMIESDKHNRS
                     FYNMSQKEFEDVFFMYRNRFRDLSKDDKVEYVSIFKNFLRKAGASLMHPHAQILSMSF
                     IPPEITNELLVSKEYYDSNKSSLYDDLIENEISLDRRVVYNGEAFLVLIPYATKYSGE
                     VRIIFKDKIEFGELNDNNIQELSTIFEKLFKKLYNINGYMPFNLCIHTHPTKIETKSY
                     FNVHMHIIPRKYNFGGFELGTNMYVSSMSPEDLTKKLKID"
     gene            38827..40023
                     /locus_tag="KPD58_000482"
     CDS             38827..40023
                     /locus_tag="KPD58_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG0264269.1"
                     /translation="MNYSNRVSAMQASPIRKLVPFAQAAKDKGIKVYHLNIGQPDIKT
                     PKGFFDAVKNFDSEVLEYATSEGIPELLEALQNYYKTYDMNFEKDELLVTNGGSEALL
                     FTMMAVCDPGDNLLVPEPFYTNYNGFGQSVNVEVNAVTTKAENGFHLPSKEEILSKVD
                     DKTKAIILSNPGNPTGAIYTKEELNILAEIAKEKDLWIIADEVYREFVYDGLEYTSCG
                     NLEGVQDRVIIIDSVSKRYSACGARIGSIACKNKGLIAQILKLCQGRLCVPTLEQIGA
                     VELYKTPVSYFKEVNEEYKKRRDVLYNELMKVEGVICKKPTGAFYIVAKLPVENAEDF
                     TIWMLKEFNKDNETVMVCPAEGFYATPGLGRDEIRLAYILNEKDLHRAATLLKEGLEQ
                     YVALQK"
     gene            complement(40358..>40797)
                     /locus_tag="KPD58_000483"
     CDS             complement(40358..>40797)
                     /locus_tag="KPD58_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0264270.1"
                     /translation="KDTISFLVKTFPKIYSSLYLEDLKKFAPDYNVNKTQLRALVFIK
                     NYGVISMTDLCSKLNIEKGSLTSMVDDLTDKKYVTRKRDLVDRRKYLIDITEEGDKIA
                     TDFMDKLSDGLGEKLSKLTEEDRKKYLEAINTLQYILNKEEFR"
BASE COUNT        13312 a         6837 c         4435 g        16213 t
ORIGIN      
        1 taattttatg ataaaataat tagtatattt tgctttactt gaatgtaatt atttatacaa
       61 aagtcaaaca taatataaat gtagtaaatt tttagtttca agtaatatct cgttattata
      121 actaaatatt tattaaaatt aggaggataa aatgggctta agagacaaat tcgcgcaatc
      181 atttgctaga tcaaaaacta tgagtggccc tgagaaaaag gctaatgaaa taatgggtaa
      241 attgcttctt aagaaagcta tattacctat cgttttaatg ttcgtgataa taatcgctgg
      301 agctatgctt aaaatcaatt cttgggttac acttggcata aatttagtta ttgctgttgg
      361 tgctttcttc tacataagaa attctagtaa aaagtatcag aactttaaac cttatgttgg
      421 caatttgatt agtttagaga aaaaaggtaa aaaagagtac gttgctatta taaaacaagg
      481 taagttacca gttaagttac aaatagctta tggtggtgaa gaccttgaac atgttaagaa
      541 aaatcaaatg gttcaaataa gttataatcc agatgctaaa atagctattt tggttaatag
      601 acaataattt aataaaaaaa aggagttgtc ttaaaattga ttttttctaa tcaaatttaa
      661 gctcaactcc ttttttaatt tttatatatt atttttatat attattttta ttactttttt
      721 taattaatat ttttaaatat caaatccttc tcaataaaat agggtactat ctacaatact
      781 actttatctt ttcctaacaa tatataatct ggtgctactc tttcatgtta tacaccgttt
      841 tgtaacatac cttttatacc agtaacatct tccttaattt cctctacatc tgttttcatt
      901 atacccattt gaacaagtat atttttgttt atttcttgtt gttgtgtaga taattctata
      961 aaattttcta cagttttttt atacatgtct ctatcttctt ttttctcctg catagtattt
     1021 ttaaataata gagcacatag tatacctatt gcccctagac tactcaattc ggttattaat
     1081 ttatccatta tagcctccta ttgtctaaat tttttatact tatagcttta atttttgtta
     1141 tattcttaca tcatgcaata ttatattttc aggattatca atttaatctt ctgtacttta
     1201 aattgtaggt gcaatttata gattgaataa ataataaata tatatacaat ttctaatata
     1261 acaaaaaata agtacaaggc ttacttatta aagttaagac cttgtacttt acataaaaaa
     1321 aataactaat acatagttat tctaaattta ctattcaact ggtatttcta attctttgca
     1381 tctaattcta acttgctctc taattactgc tggtacttct tctattgttt ttttgcctct
     1441 taaaattaaa gctacatata cttctaccat tccactctca cctccagatt ttcttacact
     1501 aaaagagctt atattacttg cttcattgac agaactttga tttaacaaca tctcatatat
     1561 ttctgcattt gctataaaat tatttgttat atcatcatct tgctttactt gacctatact
     1621 tatgtctgaa atgtctttat actgcatttc tacttttcta ccttgtttga tttgttctat
     1681 tttattttta tcgaatttaa acaattttca cacctccatt ttaagcttgt ggctcgttgc
     1741 tttcagcatc atatctaaaa atcatagatg gacgttttat agctgtccat ttatctcctt
     1801 tttctactat gattttaggc tgagcacttt ttatgttggc ttcttctaat tcatagattt
     1861 cttggtaact tgagtctgat tttttgattt caactagttt catgtctttg aataagtcag
     1921 atttatttat ttttacttga actttagttt tatttattaa ttgatatata ggattgtttt
     1981 ccatcatgtc atcctttaat ccctcattta atgttactat taaatcagtg ttattttcag
     2041 taatgtttat gattgaatat actttattgt ttatcattaa aatagaatcc ttttttactt
     2101 taagccctgt ttctgattta aatataaaca tacttgaata taaatcgcct gtaggactac
     2161 tggctttaat cagtatctta ttttctccta atactatttt attttgtggt atctcaaata
     2221 taatgctatt tatttccaaa ttagtcttag tagatattaa ttcatcattt ataaacattt
     2281 caagtttttc tataacatta ttagggtctt gtactacaac tctcacttga gatattgatt
     2341 tttcaactat ttctatagat gctttacttg tatctacttt tgttatcatg tatattgtat
     2401 catctgaaag ctctatatct aaaatatcat ttagatgttc aggaattggt attttagtaa
     2461 attcatataa atcagtaatt gatatagata ttttatacat agatttattt ttaccagtta
     2521 tccatagact attatcattt ttaattacta gtatgtggtc atcattatgt gacttaattt
     2581 ctttaacatt agttaatact ttagtaaaag ttcttattat cttatctttt tcactttctc
     2641 ctagtccaaa taatccattt gtattgtacc cttgagcgta caactctccc tcattagtta
     2701 aaacaagtgt cattgagtta tatatactta tatctttaac agaaggtaat ccagtaacct
     2761 tttgtaactc tcttacatca ttattgtggc caactcccaa ttggtactca gtattctttc
     2821 catatgacca tagagtatta tctgtcttaa taatatagga gtagttatct ccacaagcaa
     2881 cttttttgac atctgatata tttacttcag taagagttaa taattctata tcttcaaggg
     2941 tttgattaat ttctttatat gaattacttc ctattccata taacttgcca tccttagcaa
     3001 cagccaatga atgtgttttt gatatatcaa tatcagatac ctcaaatggc aatattattg
     3061 aagtatactt aaaattatct ttaaatatgt ttttataatt tatatactta ttatttgttt
     3121 ttatatactt atcattaaac attactagct tttcattttt cttattaaaa actattttat
     3181 ctgatgtgtt atctgtttta aaatatgaat gtagggaacc ttcaagagct tcatttttat
     3241 cattatcata tctatggtga atacctaaat gattatatct attatctcct gttgaagaat
     3301 aaaattttcc atatatatct ttaataaata tagtattgta actagaaaat acagtatcaa
     3361 catctttctt tatagatact ttataatatg gaactgacat atccctataa gattcatcag
     3421 atataccata agtatctcta cctgatgata tcaaattgtt attattatct atataaagtg
     3481 tattattagc tgttacaaaa acatctttaa catcttccac actatttgta ttattattat
     3541 ttgctatact tccatatcca ctaagacctc caacaaatag tttattgtca tttgttaaaa
     3601 tggctgaagt atatccatta actactattt tttttgcatt tataatatta ttaattactc
     3661 gtataccctt tatatcatta gtttctgttg aaaagaatac cctctctttt ttccctgtag
     3721 tataaataac attgtcattt gttataaaaa atgtagcttc acttcctgct gttacaacag
     3781 attttacatt ctcaatattt atttttgtaa atgtacttat acttccttca gtaaacccaa
     3841 aatatccatt tacaaatcca catacataac atgtattatc atttttaaca attattgata
     3901 catcattaca tatatacaca tctctaactc catttatagg tatacgtgta aatatattcc
     3961 tacttgtagt atcacccaat cctaactgtc cttttccatt attacctgct ccatataaaa
     4021 gaccatcatt agttaataat agagatgtgt ttccatatac agctacatat tttacatttt
     4081 caacatttat tttagtaaat acatttctat ttacattatc tcctattcct aactgaccag
     4141 aattatttaa accaacagca taacaggtat tatcattttt tatcgcaaat acatgtttac
     4201 tattgtcagt aaatctattt acatttatag attttatatt atcaatattt attttagtaa
     4261 atacctttct atttatctca tctcccactc ctaattgacc tacatcattt aaaccaacac
     4321 tgtaaagcat attattatta gttaataaat atgtagactt cgaatggcta tatatttctt
     4381 gtatattaga agatatttca ctactgctat tctttttatt gtaatttatc ttattattta
     4441 aatttaaact actattaact gatataccat gcgtaccctt agagttatat ccacatataa
     4501 ataagctctt atcagttaat attgcaaatc tagttttatc tctacttaaa tcataaggag
     4561 gtataaatat attttttata tttttcatta cctttttata attagtggca ttatatccat
     4621 cttgtgtatc tgaaacatct agttcttttg cactttcaga tatattccca aaaccatata
     4681 acgtgttaga agataaatac aatagagctt tatataaaaa tatggcttta tcagaggtaa
     4741 taggacctga atttatatta gatgctgcaa tattattaga tgattgattg ttaagataat
     4801 tccttccata ttgatgtata taagatgaac ctccagaaat aagtaaatta tctggattta
     4861 cattttttaa attacttgtt gattctccat aatacaaata ggcaaaattt gtagaatatg
     4921 atgattgtgt acaccttgtg ggataatagt tatatatgta attactaata tatccagcat
     4981 gtgtaattcc ttgttgttga actctataag tagaatcact tggctttgtc cataaagtac
     5041 catggtatcc agttaaaaaa gaaccataat ttgtacataa tatagccata tattcatata
     5101 taataatatt attaatttta gtaatatcat ttgtacaaga atttgtacta taacttgctc
     5161 ctgaaggatt tacataaaac cagttattta ttaatgttaa atttatattg actccatcat
     5221 taggattaac acccaataaa tagtttgtat ttataccagt tgcatatacc tttttttcgt
     5281 cagccacaat aaatgttgca ttatatgaac tatatgcatc tgttacattg ctaacaccaa
     5341 tctcagtata ttgacttgct gatttattac taagtccatt aacataactt gaactctttc
     5401 cagaagtata tatttgctta ttattagtta caattaatga aaaccagttt gataaatcag
     5461 tatttataac aacttttttt acattatcta tagatatttt cttaaatata tctgaattag
     5521 aaggaagaac acctgctatt gagttactat ttggcccaga ataataacaa gtgtcatcat
     5581 ttttaatgta gaatattcct ccagaaatat tttttgatgc tactatagac tttacattag
     5641 agatacttaa ttttgtcaac tcatttaatt gacttggtaa gtcagaaccc attcctttgt
     5701 atctattatt tcctagacca tatacatctc cattccctaa aataaccaga gtacaataag
     5761 gactaaaaac gacatctctt acagaatgta tatctagtgg aagttcaata tcctttattt
     5821 ccatttcttc ttttaataaa gttaatttat cctcagattt aattaaattt atataattac
     5881 tataactata tttttcacta tccattttct taaattctac aatgtgcata tcatcaaaaa
     5941 acgaacctaa ttgtccataa gtattatctc ctgtacaata tgaatcatta tttgaatcaa
     6001 ttagtatgct atgtgataaa ccaatgaaaa tattcttagc tacaatattt tttaaatcta
     6061 taaatgaatt atctatattc tcagagtaat ttgctatacc taactgatat atatcattat
     6121 ttccagcaac ctttaaagta cctttgttat ctataagcat agtgttacca tgaccacatg
     6181 caatagtttt tatatttact tcttctatct tagtaaatag tgatgattga agtgcatttc
     6241 caaaacattt ttcctttcca caaccatata tatcattatt atacgtcatt aacacagtgt
     6301 gttcagaacc acaagctata gtcttaacat cattaataga aactaaacta aataaatctc
     6361 tattattctt atctcctaaa cctagttgac cttgattatt aaaaccagta gaatagcatt
     6421 tattgtcatt ctttattata aaagctgatt tattatatga aaatacagcc tttacattgt
     6481 caacatctac ttttgtaaat gtatttacat ttgcagatgc tccattagtt cctagaccta
     6541 attgaccata caaattattt ccagcaacat ataaagttcc atcattgtat aaaagatatg
     6601 ttgattgaag tccacatgca atatccttta cgccttttat ttctggaacc ttctcaaatg
     6661 tagaaactgt tttccctact ttttctaatc ccaatcccat ttgaccatct gtatttatac
     6721 ctgcgacaag taattctcca tctgaagtaa gtataactgc atgagattct ccacatgcaa
     6781 cttttttaat attggtagat atatttggta ttttcgtaaa taattttctt gttgtattgt
     6841 cttctcccaa tccaagttga aagttactat tatctccaca tccatacaag atattattat
     6901 tttttagacc aaatgtatag ttacaacatc cagtaaaaaa attaataaag tcataattat
     6961 catacccttc atcaaattca tcaactacca tatttttatc gccaaagaaa ttacctcttt
     7021 gaagtagttt agttcttttc attataaaaa cctcctaatt atcttatttc atacgaatat
     7081 ttatttaaag ttatatctgt ctgaccaatt gaatatgccc tattagattt tgtttcaaca
     7141 aagttattat ccatttcatg tatatatcta actctatttg cattaaatgg tagcaccaca
     7201 ttcttaaatg taacactacc aagttctcca gttatacact ttctatttaa atctaactta
     7261 aggccttcta tagctattat gtttttagca ttagttaagg tatcatacca atatccagct
     7321 tcatctacat ctatttctga ctctaattct aagtatatta agatatctaa tactttttca
     7381 gataatttat tgtaactatt ttttaataca gatagttcag tttgtatttg acctcctatt
     7441 acatcatcaa tagatatatt tttaattttt tctactacct catttatagc tcctgataag
     7501 tttttagctt ctgtagataa gagttcttcg ccaattttta cttttatttc ttttatattt
     7561 ttatcaaact tatcatctaa atcttgaata tccttttgag ttacaaaaac caacgtcggg
     7621 tctacttcta atgtaacatt tgaagtattg gacaattgta aaatcatttt tacaaccaaa
     7681 tctttaatac tgccatcttc agcacgtggc ttatacgtct ctggatactt acctatagct
     7741 attatattat tctcattatc cagaacagca gcttctctta tcataaatcc accaactgtt
     7801 cctggtaaaa tagtctctat aactatccaa ttaggatttt tttcatcaat tcttagagaa
     7861 tttatatttc cttcccaaac tacattcttt aaagcagttt gttcctctgt tggctcataa
     7921 tattctccat tactatctcc aacttgaaac ttggttaatc caacaagctc tcctagtgca
     7981 gttgcatttg ctattttttc tctaccaatt ttggttaata ttatataaaa tttttcttcc
     8041 aattatacca cctccgattt aggataaact actacatttt ctacacctgt atcatttgca
     8101 atagctatat taatcttaca attattttca atgcatttaa tgcttaaagg ataaaccttt
     8161 atttcttctc tacttatcat tccagctcca cagtttacaa cagcactaca ttttataatt
     8221 ggttcaaagt atggatgaac ttttacctct tcactacata ccatacctcc accacaatag
     8281 accatacttt tatctagtat tattggttct aaatagtgta ataagtgagc aggttttact
     8341 ctttcaatca tatcgctaca atctaaaaga gttttatagt cacagttatt tgctataaaa
     8401 ctcaatacga atgtaaattc atcactataa acttttatat ctgtttctga ttttgtatat
     8461 gcctcacata tactttttat aacttcaata gtactagtac ctctgcttct cattttagct
     8521 tttatattgc tacgtcttgt gtcaaaatca agttcttttt tttcaatgca gagtatgtct
     8581 tcccacatat ctaatcccca tgttgcagtg tcaacaaata attggttaaa ggtatcatca
     8641 atatcttctt taagaatatt taattctgta tcgtatgcac cttgtatctc ctctacaatg
     8701 taatttctat caaatgatgg tagtttatca attagcttca ttgattttcc acctcactaa
     8761 aattaacagt tgttacacta ggtattttat cttcattaat agttatatta tctgtacttc
     8821 catttataag taggttactt aaatcgtgta caccagtagt atttataagt attcccatta
     8881 cttttgtata gattatctct cctctaatat ctctaaagta tgtatttata ctttctagga
     8941 aagattcttt tacattgtct aatgtatatc catcttctag tttcattact gcacttatac
     9001 ttatttctat tggtaatggt gtcacaactg ttatagttgg tccaataggc ttctcttcat
     9061 ctatatgttg ctgacacctt tcaattgttt ctgtatcaac agcttgatta tttttcccaa
     9121 atatcaagac cttaactgtt cccggaccat cccatcttgg ataaacctta acattataga
     9181 ctccatctac ttccaaagcc cattcttcat agtgagcttt atttccactt gtagcttgat
     9241 ttctttggat tttataaaat ctttctttta gttcttcatc tgtctctata tctgtaccac
     9301 cttcaaaact tcttgtgtta gtaatctttg ttactccaga tatattatca actagtttaa
     9361 attcacaatt tgtaggtaaa ttatatttct taccaacttc cagagcttga actgggctat
     9421 tgtcaccttc ttcactacca atagttacat cttttattac tacaaatagt aaatctctat
     9481 atgatattat tgtgccatta tttattacag tacctttctc tccaataaat tcaacttctc
     9541 catttgactc tgtacctaac tttctatata caccaaattc attgactctt ttatcaagaa
     9601 actggttata tgtgtcttgt ataaaagcca ttttatgcat ctttgaaagt tctagatata
     9661 tcttcgaaag ttctagatta tttccagata ccatgttgtt tagaaaagaa ccttctcctt
     9721 tataaatatc aagattaata ttttcaagag ttctattttt tattacttca tatgtctggt
     9781 cactatacat ttatttccac ctccccatag atagtggata cttttacatt tgcactcaat
     9841 atgtctctgt taaagttagc actttttacg tttacttcta atatatatgg atttattaga
     9901 agggcctctt ttatgaatct actagcttca ctttctgtaa gaccttttgt atatttttgt
     9961 cctattagtt ctgaaagctc tgtcccatat tctaaagagt atatctcatg ttcatatcta
    10021 tttgtcttta tacacttgta tatccaaact tttaaggctt cttttttttc aattatttta
    10081 aagtcacctt tttctaaaat aggttcatct ttttcaaaat cccatgccac ttcacgaaag
    10141 attggcaatt cttctgtttt aggtaagata taatcctctg ggacacctat aaaaggaaat
    10201 attgtactca ttataagctc accaacttac ttacaacagc aaatttttcg cctattctaa
    10261 acattattac catatcccca gattcaaagt tatctataaa aggatttttt acttcatgct
    10321 ggtgttcttg atttgtttct gtgtcaaatg tttctagctg tctatcaatc atccaactat
    10381 ctatcaaaat atcttctttt tctaatatga tgttatttgt ctctattttt aaatctggta
    10441 atttactttt aatttttcca ataaaaaaag aaggttgatt atgatacttt ccttcttctc
    10501 ttattattcc tataaattca tttattggat tagccattat atcaccatcc ttttataata
    10561 ctctcctagc tgtgttaaaa tctcttctac tacttaactt acttatttta actacatcac
    10621 ctttttgtgg agcatgtaaa aattctccat tacctatata taatccaaca tggctgactg
    10681 gattatgaaa aaatactaaa tctcctgctt gaagattttt ttgttctact ttcttgcctt
    10741 ttttagattg ttgatttgac gttcttggca aactaacatt aactttttta aaacaataga
    10801 ccattaaacc agaacagtca aaactacttg gtccatttcc accccattta taaggtttac
    10861 ctagatgttt ttttgcttct gatattacta ttttagcttt atcagataca ttaccagaat
    10921 aaccaccaat aataattttt ccttttcttc ttccaaattt actagcttct tcaacatttc
    10981 caaataaaat atctatatgg tatgttccat tcttttctat agttattgca ggtcctacgt
    11041 catttacttt atagactcca tcacgaccag aaactcctgt aattacctgt atttcatctc
    11101 catagtttaa taaaggatgt ttatttaaaa actcttttgt ataataagtt tgctcatatt
    11161 taccaaccat aggagcagcg caagtttttt tagatgggtc aagttttttc tttctacaat
    11221 ctgtatctcc accttcttct tttctaggac agtaagctgt aaattctgct gtaaactctt
    11281 ttcctcctgc ataatcttct ccccctaaat tactttcttc cttaggttca tcctgtcctg
    11341 ctgacttttc atccataaga ttttgaaaat taagttcaag ctcaatttga tattctccat
    11401 tttgccaagt atgtttgtct gtatctatat aaaatagtcc tacaagcttt gtataagaat
    11461 ctttaacttt tactcctcta ccagttatac aacttacatc tccataacct ttaagagaac
    11521 agcttttttc tatcccatta aactcgctat caatatctac atcttgattt tcttgttgct
    11581 gaattacttt ttgcattatt acatttactt ccttaaaaat ttcattatct attttttcgc
    11641 taatcttgct tccatactgg tctaccacta ttactttatt ttttacattt tccatgctct
    11701 ccgaaaaggt ggtatttata atattaaatc cctcttcaaa cataacactt aatgtaacag
    11761 ttcctttttc aataacatta aacttatcta aattggcctc tatcatatac tttttctttg
    11821 tctttttact tgcttctgta tatgcactca ttattgtgtc ataaccattt actcctataa
    11881 acatctttgt atacttgaca ttggtctttg ctattattcc aactgaaagc ctattttcag
    11941 caaatacttg ctttgctatg tcttcaacta acttatcttt aaaattgtat gacacttcac
    12001 tttgtgtaag taaaaatccc atatctttag atacaaaact aatttcatta ctgcttgaat
    12061 ctttagacct attaattatc attcctctaa agagttcttt ctcatctaca tagaaacaga
    12121 ctgtactagc tattggtata tcgatttgtt ggaaatttac atcaaatgat gattgtatta
    12181 ttgaaaactc tagtgtcctt gatggagatt tataatcacc tgaccatgat accttgtcta
    12241 ctatgtcagt tatgtcatat atacttccgt tttttatgtg tacccaaatc ttaataataa
    12301 aaaatcacct gccttttcat ttaattctat cagcagttat ttataactta ttatctataa
    12361 tagttattgt tttggagtta cttttggtat ctttattctt ttatactctt ttaaatctaa
    12421 tgtaaaatat acatctcgag tcccatctct ttcttcataa ctaaaccctt caattatgac
    12481 ttccatattt atatttgttt ccgtaattgt aaatcttaat ataaaacctt ccttcatcca
    12541 tttttctatc ttatttacac aatcatatgg tgatggaaaa cctgtatagt cacagaaagt
    12601 atagtcttta tttggaaaaa aacttgatat tgaaatgctt ttaagagcta caccaccaaa
    12661 agttgctatt tcacctattt tgagtatact agaagagttt acagcagctt ttccattaat
    12721 actaaaggaa gatggaaata ctggaaatct aaatctatct tcagcttgtc taagccacat
    12781 ttccattata agacaccccc taactgtgcc aattggatac ttgcaactaa ttttgatgtt
    12841 atcttttcta tatcagcttc ttctctaatc actattgtat cagctaactt agctatgttt
    12901 atatttccac cattttttct gcttctatat tgatttgctt cttgtttagt taaaaccatt
    12961 tcaccttcat ggagtctggt ttgatagtca ttataaggaa catagttcaa tccaaatgca
    13021 ttattacctt tcttacctga ataagcttta ccagaatcac cagcctttgt tccagcatat
    13081 ggattatctt tgtaattttt tttactcttt tccactagat ttaaaactgt tcctgtgatg
    13141 cttccatttt ttatcttatt aatcatagtt tgccaccatt tacatatatc tttaactatt
    13201 ttacatactt tatctaatat gttaaaaaaa gctcccaata ttggctctat aattttccat
    13261 ccagtttcca acagaggtcc taaggtctcc catacagttt tccaaataac acccagtgtc
    13321 tctataaaat tgtttatttg aggtgcatat tcacctaaaa aagtaaaaat tcttgtgata
    13381 ataggactaa cagcttcaac tacaggtccg attagttgga atgctgtagt tactattgta
    13441 ccaaatatac ttattaatcc ttgtgcaatt ggtgatttat tgtttatact atctagtaaa
    13501 ttatttacta agtttactac tggaagtaat gaatctatta atgctgtaaa atcgatattt
    13561 tgtaatagtg tacttcctgc gttaacaatt gcttttggtg cattccctat cattgttgct
    13621 gcatgatttt caaaacgttc ttgcttattt tcaaccatca ttttacgctt ttcagcctca
    13681 ttagaaattc gatattgagt agaatcagaa aactcttttc cattttttac acttctagat
    13741 tgcatctggt catatttgtt gccaataatt atcttaccat tcattacatt gcctatttta
    13801 ccagccattt tttcagataa gccagctaaa ttgcctaatc cactttctat aaaagttttt
    13861 attttcttta gtccttgacc taaaatatca tcaaaaggtt taacaatact cttcaagcca
    13921 ttgttaatgc ttgtttgagc tcctgatatt atacctggta ttgtattact catttcttta
    13981 gctaacccca tctgctttac acttatgcct tccaatgctt catcaaaagt tttataattt
    14041 tctcctacca gagtattaag agattccatg tttccttttg ctgcacttaa gaaagctgct
    14101 gatacatctt gttcactact tgtattcata tttaaagctc taacatctac catagcttga
    14161 gttatatctt tttctttttt gccaccagta atattactaa tcattttagc tttagttccc
    14221 attgcaacaa catcttcagg gtcaaaaggc gtatcatttg ccattcttac taaactgcca
    14281 taataatcat caacatctat ttgattaact tttccagtat ttcttttcat tacactttct
    14341 agaacaactt tctgattttg ttcctgacca agtaaatcaa cacccttttt cacagcgaca
    14401 cctatagtag caccatttgt caaatcagat atcttccctt ttatcgaacc aagtattcca
    14461 cttgccatat cctttacagc tattgcacca ctccatacct tacctgcaaa taatctcaat
    14521 ttgggaaata ctttacttat aatgccactt gccatatctt ttatagctat tacaccactc
    14581 caaaaagtat ttttaaacaa cattaactta ggcaaaattc tacccatagc actactcacc
    14641 atatctttaa gattaatact tccttgataa ggacgtttct ctacttgttt taattttccc
    14701 aagatactac ttatcactct cttagtcata tcttttgctt ttattacgat attccaaatc
    14761 ttgccaccaa tcttttttaa atttccaagt aatttatttt tagttttttc tacttcatca
    14821 cttttagctt taaatattaa tataatcata atatttgaat taaactcatt tttcttttga
    14881 atcaacttat taaaataatc aataatcata gctatagtta ttgctagtct cataatactt
    14941 ccatttaaac ttccaataac tcctgaaaca gcatttatgc tagaacttaa ttttgtcata
    15001 gttgaactta cactactagc agtagtttga acttttctta acgaggaatt taaactgttt
    15061 actctattac ttgcacttcc aatagaattt tttatgctac tagccattac attagtggaa
    15121 agtgaaacag ccacagttgc tctcctaaaa ttatcagtta ctgatataat ttgattcatt
    15181 tttggtgtgt aattatctgt aaggtacata tttatacctt cttctctcac atttcccatt
    15241 acataccacc tcctgtcaaa ggacacactc catatccatg tttcattttt tcaacttctt
    15301 cctcaatttc taaggcgaaa aaagcttcaa taagctttaa ttcgccttta tccattgcat
    15361 aaaaaaggga cggtcttata ccttttttct tccaataata gtacattatt gtagtcctgg
    15421 tatccgtccc tattagtttt ttacctcttc tatcactgca tttttaccat aacccataac
    15481 atcttgaagt attctatata agttggtaat ttcacctggt aataataatt ttcttgctaa
    15541 ttcttttggt gttggagcct taaatttatc cattagctcc ttatttttaa ataaaagctt
    15601 tccatccaaa tcacaaacac cttctataat tgtaagcatc tgcatctccg gtaagtctat
    15661 atccgttttc atattttcat caattttaag acaagtattt tgtatttctt catctttact
    15721 tattgttaaa gctctacaca ttactttaaa ttcctgccca aatatagttg ataatctctt
    15781 aagttcaact atagtacttg gtctttctaa gttttctgta tctgaattta aaagtaaatc
    15841 tactatgttt atattttttg ataatccatt ttcactcatt attatttgct cctttatcaa
    15901 tcaaattcaa tcaactaact tttttactta ttaactatct gaatttatta agtatatact
    15961 tactaaatat cagtataaaa ctaaatataa aactaaatta tatcaagaaa atcatactca
    16021 gtaaatgtga aaggagcttc tatctctcct tttactccaa cctcccaatc agctaaagtt
    16081 aaatcatcaa atgctatatt ttttactgct attctttctg ctccataaga atctgggtca
    16141 tttaattttg atatagcaac aaatctaggt tcagaacctc tctttatctt ttctccaatt
    16201 aacttgtgca ttcttgaact aacgtggtat agagttattg agccttttcc tttatatccc
    16261 atatactttg tatcagtacc catttgacct gctattataa tatcttcttt tgtaaattcc
    16321 atctttgctt gaaatttctt tacttctgct actttgtttc catcaagcca aagttctccc
    16381 caagtaccac tcattacatt tctagcttcc atatttgcca tattaataat cctcctatat
    16441 ttctattgat aaatctatat cttccatagc atcaagtact tttatttttg cttttaaaaa
    16501 tactttagaa cctgtgttag cttcttttat ttcttgtaat gtcatataag ataaatctac
    16561 tccttttgat tttaaatacg atttttgtgc ttcaaaatct atttcaacag tagaatcaga
    16621 ttctataagt gctgattttt ctaattcttc taaataactt tttatagcta ctatcaataa
    16681 acatttgttg tcataactgt tagtaacctt tcctatatag tcatctatta tcacctttct
    16741 tatgtcacta tgtataatat ctaaagtgtc aactattttt attttctgga acatttctcc
    16801 tttttctgct gttaattcag ttaaagaatt tactcctcta gcaattctta tagctcctgc
    16861 ttctttaata agtataagct ctcctttatt aacccttgat tctgcatcaa ctttcgtcat
    16921 cttaggtata tcgactacat cactaagttt agtataagtt actgattgac ttaaaggtgt
    16981 acctgctata agtccagcca ccctgcttgt aaactcatca acactgtatt tcttttctcc
    17041 aactaacaca tcttctgtag taaaattaat tatcccttca tgatttccta caacttttcc
    17101 taatacagct ttaaccttaa ccttatctat atctctaagt ttaattatcc aattttttat
    17161 agcagtctta tcagcttcta ctgcttttgg catacataga taattaaatt ccttagtctc
    17221 taaaaaatct aatgcagttt gaatatctgc ttctccttct attacataaa ctaataattt
    17281 atttggagtg ttaacatttc ccatcaaagc taaatttata tattctttat tttcagcaga
    17341 taaatcaact ggtatatcct ctttttcatg tatttcatta agacctagtg ccttagcatc
    17401 tttaagtacc attgcaatta ttcctctagc tgaacgttct ttaacagttg tagctagctc
    17461 cttaaatgat atgttgatac ttggtaatcc tatagccata ctatcactct cctaaattta
    17521 aattgatttc tttcatattt tcatatacta catcttcttc atatacacta tccagatagt
    17581 ttagtgtaaa tgtaaagttc aatacataaa ttgactcttc ttttttaata ctttgttcta
    17641 gctttgttat atttaatttt ctgtctgtta cctttatatt tctgttaaat agcttattta
    17701 attcatctga aatttcatac attctaatac tctttgactg tggttttggt aagtatttta
    17761 tagagataac aatatctttg ttattcgtat ttaacattac aggtgtacaa ataactgata
    17821 gtatacttac aaaaaaacaa gacccttcag agccttgcac actttcatca tcaataaaaa
    17881 tactttcagt aaagtttttt ttgagaatct caataattgt ttctaatatt tccttgtatt
    17941 taagcaaata atcaccacac tatcactaaa tattcttgct taatctgata atcttactat
    18001 aaatgatata agtcattttt aaatatatat ctcatttatt cctgtcaagt atcttctata
    18061 tttttaggta atattattct tttcaatcta tcacctcctt caatatggat ttctttcaca
    18121 ataacattat ctcacatatt ttattgccta ttgttgcaat ggtgtatata aagtgttgta
    18181 aaattgtcct attttttata atatctcact caatttaaat atcatctttt tctttaaatt
    18241 gtagcaagat gttctatcta agtgtagttt catagaaata tatgtcatat tgttttttgt
    18301 cttactatta taaaaaagat taaaaaattc catttctata tcatttagac acgttagagc
    18361 attttctatc ttctcttttt ctatttctat atcttctttt tccattttta attttcttaa
    18421 tctttcctct ctttttaaca cctcattttc cacagaagag cttatgttat atgtgttact
    18481 agtcttttct gtatatacca ttgctccaca tccagagtat tcattctcac atctttttat
    18541 gtccaagtta atattcttta tctttatatc caaatactta taattatgta atcttttatc
    18601 tgtttcttta aataatatat cttttttgtt ttccatagta cttccccttt tttatatact
    18661 tattattagc ttcaaattta caataatctc tttagtgcac agttttttac taatctcaca
    18721 actttcaaga acatattgtt ctataactgt ataaaaaaaa cttcttctat attttgttct
    18781 aaaatactag aaatcttctt agccataaga agtccaggat tttttatacc tctttctatt
    18841 tgagatatat aatcatttgt aattccagcc tttctggcaa gttgaatttg tgtcatacct
    18901 ttgcattttc tataatattt caagttattt ttaaacattg ttttatacct ccattactat
    18961 taagaatatt ttgttcttcc tcacttagat tatatagaat tatttgttct ttatcaatag
    19021 attttagaaa aaaatattct tttatatttt attagtttta ttttttagaa caatttgtta
    19081 taatctatac ataaggtggt gttataatgt ttgccaaaag attaagagaa cttagaaagg
    19141 aatttggatt gactcaaaga gaacttggag aaaaagtagg cgtttcccaa agagtcttag
    19201 ggtactatga aacagaaaac agatttcctg atgaacacat tttaaataaa ttggctgatg
    19261 tatttaatgt atcagttgat taccttcttg gaagaacctt agttaaggaa aatattgata
    19321 cagtagctgc acatagaaaa aatccacatg aagagttacc tgaagaagca caagaacaac
    19381 ttaatgatta tatagaattt ttactaaata aatataaaaa aaaataaacc atatgatgag
    19441 cagtctattt ttctgctctt ttaatataat ttaaaggcaa acatacattc tattttaggg
    19501 ggatttttat gaataatttg gataagcttt ttgaattagc ttctcaagaa gagataataa
    19561 ttcattatac cacttatatt gcaggtgatt tagaaggact atatataaac aaacatggta
    19621 ttaaaattat atcattactc agtaatttaa aacaaaactc aaaaaaattg acatccatct
    19681 tagctgaaga acttggacat catttcacta gccttggata ctatgtatcc tcttataacg
    19741 attattatac aaaaattatt atagataagt gcgaaaataa agccttaaaa tgggcttgtg
    19801 agttcttaat tactgaagaa gatataataa acataattaa ctctggcatc acttgcgttt
    19861 atgaaatggc agacatactt aatgtggata ttactttctt tcaaaaaaga ttagaatttc
    19921 tatcattaaa aaaacagtct ttacaacttg gaaataataa atatttaata ttaactaatt
    19981 tgccgtattt ctacatattt gatccaattt cataataaaa atagtagtaa ttttaactgg
    20041 cttaacagta aaatctatat atttatttta acaaattttg gtttctataa attcaatatt
    20101 aatatataat aatgatatca tattatttaa aattctaaaa acaaatatat gcttttgtta
    20161 agaaaggttg tttgatgaat gtataaagaa aagttgtttg ataattattt taagttctta
    20221 gcactattat tttggccaat tatgtggtat aagtggatag taatttcaaa tggtactctt
    20281 gaaaatatgt tatttaccat atatgcaatc gtcgcaattg ttttcatcat tttatattcg
    20341 gtgtttatga taaaatataa agacattaca caaatagatt tcttttacag gataagtact
    20401 ctacttgctt ttatatttac actttttagc tttttaatat acccaaagag tttatttttc
    20461 ttgtatttaa agattatttt tactggaata tacctttatt attcaatagt aaaaactctt
    20521 aaatttaaag atgacgaagg tgtagtgggt attatgagct cacttttatt aattgtaata
    20581 actttgttct actaaattta attctatttg acttaatatg gctttaaata ttagacatct
    20641 acattatttt ctgatattat ttaagatggt agtcttatat taataatgaa atacactatg
    20701 aaggagttaa ctattatgga aaaaaaagtt ttagcaacag ttggagaaaa agagataact
    20761 aatattgata ttgaaaatgc attaaaatca ttagaccctt atcaagctat gcaatttaaa
    20821 actgaagaag gaaaaaaaca cttattaaat gatttagtga atcaagaatt attcttctta
    20881 gatgctaaag aagaaaaatt agatgaagaa gaaatattta aattagaaat gaaaaagata
    20941 gaagaaaatg tattaaagca atttgctata aataaagttt tatctagtgt taatgtaaca
    21001 gaagatgaaa aagtaaaatt ctttgaagct aataagagct ctttctctaa accagaaagt
    21061 gcaactgcta aacatatatt agtagatagt gatgaaaaag ctaaagaaat acttgctcaa
    21121 ataaaatcag aagaaatatc ttttgaagat gcagctctta aacattcttc ttgcccatct
    21181 aaagatatgg gtggtgattt aggaacattc ggtagaggac aaatggttcc tgaatttgaa
    21241 gaagctgtgt tctctatggc taaaggtgaa gtaagtgaac cagttaaaac tcaatttgga
    21301 taccatataa taaaattaga agaccttcaa gaaagtacag agtctacatt tgatgaagtt
    21361 aaggctgaag ttgaaaaaag tttattatat caaaaacaaa atgaagttta tggaaataag
    21421 ataaatgctc ttaatgctaa gtacggaaac cttgtaaaat acaacgatta atcataaggt
    21481 catatataaa aaagctgtct tttcacaata tttattagaa aagacagctt tttatattaa
    21541 agtttaaata ttcagtttca tattatcatt tttttattgt aaagaactca gcttaaaagt
    21601 ttaaataaga tttttaatta tcattagcta aatttttata tccacgtatt atctcataac
    21661 agtaacctat tacatctctt ctttttatta tcccaataaa tgtgtcttgg tcatctataa
    21721 caggaatgaa attttgattt aaagatttta taactaaatc ttcaatatct gcgttgatgg
    21781 aaacaggaga attatccatt tttctattta tatctgtcac tggaacatct tccaatccct
    21841 ttaagtccag atttaaatca ttttttaatg tccataaaaa atcaccttct gttatagttc
    21901 caacatattt accatcctta cttattattg gaattgcaga gtatttgtga tattccattt
    21961 tttctagggc ttgccttata gtgtagtctt catatatata tgctacttca ctttttggtg
    22021 ttagaaagaa taatatgttc attacactac ccaccgttac tgaaaactta taaatattac
    22081 tcaatattta taatgttact tttttgcagt aactttctcc tttcttattt tctaagtatt
    22141 cttaatacta tacgctgtaa caagcgtttt tcctttgctc attttaatct tattatgctt
    22201 tattaatatt tttatatggt ctataagtat cacttattct ctttttagaa aatttactaa
    22261 aaattaattg acttaaactt agccataata attttactct acactattaa ttttaacata
    22321 tttatctcat tattaatgat tttattttaa taattttaat ttattttgct attaatttta
    22381 tttacaaaaa ctgaaaaaac cgctaaatga ttagcggctt atctctcata ttactagaac
    22441 actaatatat atgaattttt aattataatc taactacgtt agcagcttga ggacctttag
    22501 ctccatctac tacttcgaac tcaacgcttt gtccttcttc taatgttttg tatccatctc
    22561 cagttatagc tgagaaatgt acgaaaacat ctccttcacc ttctacagat atgaatccaa
    22621 atcctttttc gttgttaaac catttaacta caccagtttt cattaataaa tcctcctaaa
    22681 aaaacacaaa tatttagcta ctcttttctg taaccttatt actactctat catatatagc
    22741 tatgtttgtc aattattatt atttttatac attaataatc aattttttta ctttataatt
    22801 ttaaatgatt attatctttt acactttatt tttctttata attttttaat aatttatttt
    22861 tattatatgg aatcttatgg acttatcact ccagcctaaa tcccatggca ccaacattct
    22921 atcagtatta tggcaagtta ccaaaggata tcctcttgag tctaatcctg tcactgtcga
    22981 aacatgtgtt attctgcctt tcttctcata accaacaaaa tcccctggac gtaaattata
    23041 agcttctttg tatacttctt cataggaacc tttagcaatt aatgaacctc ttccactata
    23101 cagtgcataa tttttaaaag cttgtgcatt tacccatgct tttgtacctg ctctctcgct
    23161 ataattccaa attgagttct ttttgaacct tccactttca aacattatct gtgatgcaaa
    23221 gtttgcacaa tctccacctt ctggattata atccctatag tttggattaa atttcatacc
    23281 atgttcttca tcagctgcgg ctccacagta tttatgtgca taatctatag cttttttttg
    23341 ttcatctgta agctgtaact ctacaccatc ttgttgacca atataatttc ttatatcatc
    23401 agattttata ttttctaaat ttaaagagtc tgcaaaaggg tctgtatacc actctttagt
    23461 tatgacatat tgattatcta taatttttac atttagataa tgatatgtac ctattctaaa
    23521 ggcattttct ttgtcctttt gattttcata tgaatactta tattcagtgg atactgaaca
    23581 gattatacca tagaggtctt tttctttttc ctttattttt ttaatcttaa cccttgtctt
    23641 tatgtcttta aattttacac cttgtttact agaccagttt tctaaatatt tcatcttttt
    23701 tacttcattt tcataagccc acagaccaaa ttttttatcc gtttcataaa gaccttttaa
    23761 aatattttca tttttctcta atattgcctt atttctgtag tcaaataagt tttcaagcaa
    23821 tagtttatat tggtctttta catcatcacc ttcaacattt gcagattcca tgactttttc
    23881 ttccatattt ttcatactta aatcattgat taaaaataca cctgctgtag tagctaaaga
    23941 caaaaataca aatgccatta tacctttgat gtacttattc ttatttttca gtattttcaa
    24001 agctacacct cccaaatagt acaccttaca aatcaaatta caaagaatat tctgtcacat
    24061 atagtaattt tcatatttaa taattctcta ttttattatg tccaatatta aaaattaatt
    24121 gtatcaatac actgtttttt ttacaatttt ttattttata atctactttt ttcttttttt
    24181 tatagtaata tatatgtata taaatatttt ggtatcatta taattaatac atactaatgg
    24241 agggtttttt atgcaaaaaa aagtcgcagc aattaatgac ttatctggaa taggaaaatg
    24301 ctcattatcg gttgctatac ctatactttc agcattaaag gttcaatgtt gtccatttcc
    24361 aacggccata ctttcaagtc aaactggtta tcctgaattt actttcttag actttactga
    24421 tgaaatggta aaatattcaa atgtttggaa aaatttaaaa gtcaattttg atagtatata
    24481 tagtggtttt ttgggttcaa agcatcaaat tgagatagtg gctaatttta taaacgatta
    24541 tcccaatgct tttatcgtag ttgaccctgt gatgggtgat aatggagtta tgtatccaat
    24601 attcacagaa gaaatgagac aggaaattaa agaattagtt aaacattcag atttaactac
    24661 tcctaattta acagaagctt gtttcttaac tggaaatgat tataccaaaa gcgattataa
    24721 tagagatgag cttatatata ttgcaaaatc tgtatctgat ttgggaccta gcaaagtcgt
    24781 tattactggt atattagaag atgataatat attaaatttg gcttacaata gagataatga
    24841 ccatgtattc tttacatcag tcaaatataa taattgttct tacagtggaa ctggagatat
    24901 attcacatct atactttgtg gtatgttagt aaataaacat gatttgggag tagcggttaa
    24961 tactgcaaca gattttatat ataaaactat aaattacaca tctcaatttg atactgatag
    25021 aaatgatgga gttatgtttg aaaacttttt atcagattta accaatatat aaaatcaaaa
    25081 aaactatcca ttttataaat acctataaat cataaaattt aggtatttac aaaatggata
    25141 ttatttacta aactattggt atttcaattg ttaccatagc accaccagtt ttcacagaat
    25201 tttcaataca taaagtccca ttatgttttt gtacaatatt atttacaata tagagtccca
    25261 ttccataatg agatttagaa tttctactca aatctcctgt ataaaactgt tctgtagctt
    25321 ttgacaaatc tgctttagaa aaaccacttc cacaatctgt aactacaaat tttaagtatt
    25381 tattatgtac tgatacactt atgtatagtt tactctggct tggtgaataa tctatagcat
    25441 ttgaaattat attcataata gctctgaata acagttctct atctatagtt atatttttag
    25501 gcaaattatc ttgtttataa aactccacat ctaagttttt agtactagct aatgcttcta
    25561 tttgcactaa aacatctttt ataaactcac tcatactgat tgcttcttta tttaatatat
    25621 agccattctc tgtttttgat atatctatta aagtattaat atatttttct atttgagtga
    25681 caccctttga gatatattcc atgtattcta ataactcagg attttcattt atttcaatta
    25741 ataagtcagt atttccatgt ataattgtta gtggtgtttt tatatcatgt gccagtgcag
    25801 atatctgttc ctttctattt tcttctagca tccattgttt ttctaaagaa tcttttaacg
    25861 ccaacttcat tttatccata gaaaaaagga cattattaat ttcataaatt ttactagatt
    25921 ctacaacaaa atctaaatcc tgttgttcta ttttttctgt agtatttttt aaaagctcca
    25981 tttcaatatt aaatttcttg ccaaatactt ttgataatat aacaatttca ataaaaaata
    26041 ttatactaaa aataatcata cccaatgtct ctggttttgg taaatatgct cttaaaattg
    26101 gagaactata ctctgatact attttgtatt ttattataaa aacttcatct ttcttaaaaa
    26161 actttatata actttctgaa tatgcatgcc tttcttcaat atctctcatt aaattccata
    26221 cttcttttga ttctttttta ttaaaggtcc ctgaaattaa attcccatcc aaagtataaa
    26281 ccccatactc atatatactt ggaattatat cttcagttac tttttcactt gaaataatct
    26341 gttctttata tttattaaat tgtttttctg cataattagc tggtaaaatt acacctgatg
    26401 ataataatac agaaaatgat aacattaaaa ggagtacaat tgaaattgtc ataataaaga
    26461 ataaagataa atattttata aaaatagttc ttaacttttc aacttttttg ttgttagatt
    26521 ttttacctcc aagcatttgt accctgtcct ccaatagttt aattaattca taattaatac
    26581 ccaaccagtt ttttatttga ttattttata tctttttatt gctaagttat attttaacat
    26641 aataatataa ataaacagtc aatttttact ctattcttct gattttttac cttcccattt
    26701 agaaaaccaa atacataaaa ttacaaatgc taaaattgtt acaaaaccac aaattaaacc
    26761 tgttgattga tatgctttta ttgattcaac ctttatatat tcaaccgttt tagaacttga
    26821 aaaagacgac caaatagtta taaatctaac accccatgca caaggaaaat actgccaaat
    26881 accagaaccc aatccagtta ataataatgc tgaaagtaac atttcaacta tacctactcc
    26941 aattgatgtt ccttttccga accttaaact taagaataaa tgtaatatgt attcaaataa
    27001 attacaccca attaaaatta gaattgaaat catataaaag cttgatgtaa agtaagcctt
    27061 agatatacca aatttaaaac ctaatacaga cactaatgtt gctacaaaac caagacatac
    27121 taagattata tatttactta taagagtaag atattttgga ttagatgttg ttaacatatg
    27181 tttataattc cctgcataat attcttgttc aacaaacatt gaacacacaa cacttgacaa
    27241 taaaggaaaa gctattgcta taacttgtaa atatgctact atctttagta tattatcagc
    27301 tcctgaaacc ttataatacc ataaaaacaa tcccatcccc attaaagcaa tgcaaatgtg
    27361 cattagtatt ataaaatttc tctttaattt tattaaatca gaacaaaata ttcttattaa
    27421 ctgtctcata ttacttcacc tctctattat tgaaccaaac ccttgtagct aaagcaaata
    27481 atacaaataa aacaatagat attaatactc ctggcaaaat aacagcatta tttaaaaatt
    27541 cacttccctc tggtacagga agaccatttg gcagaatatg caatacaaca gccattaatc
    27601 ttgaaggaat cgaatatgga aacatatacc acatttctgt agttgagaaa acagcaactc
    27661 caacaatagt aaagaacata tttgttagaa ctgtaacaaa cataccaaat tttgaagcta
    27721 aaaacataca aagaggaatc tgccacataa atagtaaaat aattaatata cttgcaatga
    27781 tattttgacc tattgaaata gtttctttat agattatccc aacaaaaact acacctattg
    27841 cagaaataat tgatgctata aaacaacaaa taatacagta aatcattttt ccagtccaaa
    27901 cgtctttcat ttcaattggc aatggtaaaa taccacgata tttacttttt tcatctttta
    27961 atactattaa agaagatatt aatgttaatg ctcctggaag aaatgtcgta taccaccagt
    28021 tgaaagcagc attttgccct ccacctagtc cacacaaaag tatacaaaac actggaacta
    28081 tccatatcaa cttcattcca aaagttcctt tcatcttttg aaattccact ctaataaatc
    28141 tttccatatt actgccctct ctttctattt tcagctacaa ctttcataaa aagttcttct
    28201 aagttttgtt cttttgatac ttctccttga taacccatta ctccattaga aattattcct
    28261 atttcatcta caacttgttc aacttcagac aagatatggc ttgataaaat aactgtaatt
    28321 ccttgttttg gaaaagaacg aatcaattct ctaagttctt caataccaat agggtcaaga
    28381 ccatttgtag gctcatctaa gattaaaagt tttgggtgat ttaataatgc aatagcaata
    28441 ccaaggcgtt gcttcatgcc tagagagaat tgacctgaac gctttttaaa ggtatctctt
    28501 aaatctacag tctctaaaac ttcttcaatg cgagagtcag gaagaccaag taaagtagta
    28561 tggactttta aattttctcg tgctgttaaa ttctcatata tagcaggaga ctcaataaga
    28621 gaaccaatat ttgataaatc ctttctagtc catctatgtc cctcaaacat tatttttcct
    28681 gaagttggat ttaaaatccc agttatcatt tttaataaag tagatttccc agctccattt
    28741 ggtcctaata atccatatat agaattagat ttaattgcta gtgaaatgtt atcaactgcc
    28801 atctgctcct taaatttttt agaaactcct tttacctgca aaatataatt actcataatt
    28861 ttcatccttt cttttgtcaa acttatttac aagaatcatt ctaaatcata aatataagga
    28921 ttttataaga attaaaaaac aaaaaaagat gtgttaaaaa cacatctttt tttatcttaa
    28981 ttctattaat tttccattgt agtatatctc tacttcttca atccaaatgc tattatcttc
    29041 ttcttggatg acatttttaa taatattatt tatagtatat attatatttt gttcattcaa
    29101 ataatcatct ttccaagaaa ctctactttt tttatctggt tctatcaagt ttattttaac
    29161 aagattatct ttttccttaa cttctacttc catgtctaaa ccattaaaat actcatttga
    29221 taatgtatct aagattaatc gcagtttatc cttaagtgaa ctttcttcat caatctgtaa
    29281 gtactttact attttctcat cctttgaaat tatctctata ggtattttag tagttttttt
    29341 agaattaatc attttactag ttgtttcttc ttttgcatct tcatccttct ttacaactgc
    29401 tgattcttta gaatcaacta gctcaactgc actgatgcta taatccatac atgccgtact
    29461 caataaagta gtagcaatta ttaaaaaact tactgctatt ttattcataa gtatccctct
    29521 cctaattctc tttcgatact tattatacca tagtatagat tatattttta tacttttaaa
    29581 tcaatttata aattattata gtaaattatt tacaattcta agctatttga atttattttt
    29641 atagtatatt ctttctctaa attcgacaga tttttattca aaagcccttt tatttccctc
    29701 gtatttctat acttgttgct atgttcttca ttatgttcat aatcatatga atcattgtca
    29761 tattcttcat cattattaaa ttcttcaatt gcttcttttg actttatatc acaattaaat
    29821 ttatacttat ttactggtat atcaagtctt accttagaat ttggaacttc tatgtccagg
    29881 aacttagcaa ttggcatatc agagtacaag taaaattcac tttcataatt tgaatttcta
    29941 cctattttct cttttatatt tacattttca ggtatatact cttctatatt attgataaat
    30001 acatctccat tctctgaaga tatatccaat gactctgatt ctatgtttac atttttaacg
    30061 cctaaaattg actttgttga tagatgaaga ttgctattag atattaagtt taagttttct
    30121 aatctattta ctttattttc tgttatagct ctgctaaaac ctccagaaga agttttatat
    30181 gttatattat ttgaatctac atcatcagat ataaataagt acgaacttat acttgatgca
    30241 tttatatcta cattatttgg tacatatatt ctaatttctc tataatcacg tgaaaacata
    30301 ctatttatag atgtatttaa taagtggtcg aaacttctaa tctttttaat tttattttca
    30361 gattttcttt cgccttttac aactaaagtc ttatcttgta gggttacact gtatttgtaa
    30421 aattcattat tccctcttgt tgtcacacgc acatcttcat ttgaactttt tcttatcata
    30481 acatttatat tgtcaacagc tatatctaac ttgtcaatat ttactttttc ttgataaagt
    30541 ttgttttctt tgtttaattc agcttccttt tctaaaccaa agtttattag gcttggcata
    30601 actaaaatcc ctgaccatgt tgtccctatt atacctatta ccaataaaac tgcagcaaat
    30661 atagcaagtt ttttattcat gtttatcttc tcctttctta ttttctatat cttcaaggtc
    30721 cttatatatc gcctcaaaat ttatactatc atcattatat tcatcagttt cttttatgct
    30781 ttcttcttct ctattgtagt aatcattatt atatgaattt tcattaatgt ctttattcaa
    30841 atcttcatca tttaaagtag ctttttttct tttttccatc ttttcatttg cctttatata
    30901 aatatttttt ctctttatcc aatgtatata attatttagt aattgtttac ctagttttat
    30961 ggcaaataat aaaatttgcc atgcaagtat ctcaagccct acaaatgcta tggttgcgaa
    31021 aatacaaaac atcataacat ctggaaccat tgctataaat ttaatcacaa atggaattaa
    31081 tataacaaaa gcaattaatg agcatactaa tgcaatagta gcagatacaa tagcacatac
    31141 aaacgcaaat gctggaacaa ttagaaataa agataacact actaataacc cctttataac
    31201 ttgtctggat aatcctccta caccttcttc tatacttggt gtaagtttat catttatatt
    31261 ttcttttgat ttattgtact tttccttagc ttcatctttc aagatagaat aatccttttt
    31321 tacatttgat ttaaatctac taaatttacc atttaatatg tctgtattca ttatttttcc
    31381 atctatattt tcttttgatt tactatactt ttcttttgct tcatctttta agatagagta
    31441 accttttttc atatctgatt taaatttact aaacttatca ttcaaaacat ctgtatttat
    31501 tattttttcc tcttcaatat ttttttcttt agtttgtaag ataagttctt ttgctataga
    31561 ttttggtgag ccaagtgatg ctattatctc catatcactc tttccctcta taagaccatc
    31621 tacgaaatac tcttcataat ctctcaatat atcattaact tcgctctcag aaaaatcttt
    31681 ttttagataa tcctttaaaa tttcaagaaa ctcagtttta ttcaagttca ttacctccct
    31741 catcttcact tattaaaatc tcaacagcct ttacaaattc tctccactct tccatcagat
    31801 tggataaatt ctcttttcct agtgaagtta ttctatagta ttttctagca ggaccttcat
    31861 tagattctag tatatatgtt tcgaaataca aatcttttgt aagtcttctt agaataggat
    31921 atattgttcc ttcatttaca tctattactt tagagatgtt atgtactatc tcatatccat
    31981 acatatcttt cttagatatg agagcaagta cacaaatttc taaaacaccc tttctaaatt
    32041 gtgtgttcat atactccatc ctttctttaa atacacagta ttgtgtttta caaggtacta
    32101 tgttaaaact tcttatatcc tcataataac acagtactat gttctgcaca atagttattt
    32161 ttaaatattc ttgttgatta aatccttcta taaatgtata atactctaat cagatatatt
    32221 ttatgaaagt ggtgataaat tggctaaaat gaaagatgat aaaacaatat taatcctaac
    32281 tgctcaattt ggtgctggac atataagtgc tgcaaaagca gtcaaagaat gtattattga
    32341 aaaatatagt aactataatg ttgttataca aaactttata aatgcaagta ttcccatgat
    32401 gaataagcct atggtaaaac tttatgaaaa caatacaaaa tatactcctg gattatataa
    32461 ttactattat tactttaaga aatcgtttga ttcaagacat gatttatctc ataaattata
    32521 tacacctaaa ctttctgagt atattgctga tataaatcca gatttgatta tctctacatt
    32581 tccactagct gctgcttgtg taaataactt taagataaaa aatcctgata taaatatacc
    32641 tacacttaca gttataacag atgttgtaga tagtatggaa tgggtttttg aaaatacaga
    32701 tttgtatttt gttccttctc ctgaaataaa aaatagattt tttcagaaag gaataaatcc
    32761 agactccata aaggttactg gagttcctgt tgataagaga tttcaaattg aaagtaaaga
    32821 actatgttgt gataaatata gactattact tctaggtgga ggaagaggtc tatttgatat
    32881 agatgaggat tttatgcatt ggatagatga atttattgaa gaacatagta attctataga
    32941 aattacaata gttactggta aaaacaaaaa attatatgac aatctaactc ataaaaaacc
    33001 tctaaaaaat attaaagtac ttggatttgt aaacgacatg tataatctaa taagagaatg
    33061 tgacctaatg ctaactaagc ctggaggagc aactatattt gaagctattc aatcacaaac
    33121 tccagtactc gtaaaaatgc caaaagttgg acaagaaatt gaaaacgcta aatttataat
    33181 agataagggt cttggcatga tttatagtga tgacctagac cttaaaaaca tattttataa
    33241 attagtatca aatgaatttg attctataat aaactttatg aaaaaaaatt taaaagaatt
    33301 taaaacagtc attcatccag agaaaattgc tgattatata tcagaattaa tagataatca
    33361 ttacagttaa attgtactat taaaaaattt caaacataaa attaaaggca atcaagtatg
    33421 cttttacact tgagaattta ctcatagtgt ttaataatac ttgattgcct ttatcatatc
    33481 aaaatcaaat tgttatatta gtatatattt ttcttgtact gcatatacag ttgttgtatt
    33541 tcttccatac ttctctttaa ttctatttgt aaatctgaag cagtctggta atccttattt
    33601 tctatagctt ctctcactct agtgaatagt gattctctag ttaaagtatc cttcataata
    33661 ttgaacttta aatccgatat tgagttccaa actacctcat caagcgcatc catattttct
    33721 tttgtgtgta tcttgacaat acttcttatt ttttgaatcc cagcctctat tattctagtt
    33781 tttaatttct tttgccactt atctatagct ccaatcttaa atgattctat tatactatca
    33841 gtaaatacat caccttgttt taaagatttt aattttgaag catatatttc taagtttttc
    33901 atattttcat aaactgtagc cggtggtatt ccaaatcttg catttctttc ttccaaacta
    33961 taatgttcaa atacatcatt ttcatctcta tatgctctat ctttttctaa atagaaactt
    34021 tcttcaccta catttttaga taactctttt tcaagttctt ttgtactaag accactctta
    34081 gctgctgctt ttataccatc caacattgtt tgataacatc ctgctattac taaatacgtg
    34141 ttagatagag ggcttggtga ccttaactca aatcttacag tctttggatt tttcatatct
    34201 cttattaatc caacaagcac tgacctattt cttgatggaa tctcataact atgacctaaa
    34261 gatgtaacta tgcatacagg agcttcaaat ccaggaacta atctattaaa tccatcatta
    34321 gaagctgtta caattgggtt taatacttca taattgtaaa gaagtcccat aagagcccca
    34381 tatcctagtt cacttagata atcttctttt aaatcttttg gcgcaaatag attttttata
    34441 cttccatcct ttaacttcgc acttactcct acatgtgtat gtccaccact tcctgctact
    34501 ccatgaattg gttttgcttt aaatgtaact tctagtccat gtgatgtaaa tacatcctct
    34561 ataacatctc ttactaataa ttcattgtct gctgcttgta acggtgtaga aaacttccaa
    34621 gaaacctcta attgctccat agcatggttt gttcttccat ctatactaat agaactttgt
    34681 attcctccga cctctttgtg agccatttca gggtttacac ctagcttttg aagtataatt
    34741 aaacttttct ctaaacaagt tcttataata ccatgagttc ttttccagta ttgctctttt
    34801 aggctttgtg atacatataa tttctctaag tcagctttat cctcaggagt attaacccag
    34861 aactcaagtt ctgtagctgc tgttaacatt atttcctcta tatcatctac tgaatctatt
    34921 cctatgttat ttattacatg tggatattct ctaaatattt catacattga ctttttaaag
    34981 tacttatctg ctttttgaag tactcctcta gaacatactt ttttattatc atgtattaaa
    35041 aatgcaggta tctttaatgt tccaacaggt agacctactt cttcgtctat atattccata
    35101 ttgtaatcta cataccaatg acaactttta tctggcatta aatctacttt cgcattattt
    35161 aatgtagcta tattataaag ctcaacactt gaaccatcag tctgtatagc tgattccaaa
    35221 aatttatcta tatcctccaa aaataattct acaggaatct tttcatctgt tgcatttcct
    35281 cctaaatcaa cccccattaa ggaaacaaat cttatatttt tattgctttt taggatttct
    35341 cttaattctt cattagtgtg tttatctttc tctattacgt ataatagact atccattttt
    35401 ccactctccc ataattttta tgtatatttt attttgttaa atacaagata aatttcattt
    35461 tccttacatt tatgaatttt tcttgcaaat tattcgtgtt ttattcgtgt ttttatttat
    35521 attattatat atcaagtata aaaagttcgc aatatttttt attggaaaaa atattataca
    35581 tacaatagtt taacattttt acaaatatgg ggtatactaa aataaaagta tttttatttg
    35641 aaaatttaat tttatttata atataatcta tagtaaaaga aataataaaa aaatttagga
    35701 gataaacttt atggaataca taattttcga tttggaattt aaccaaggat ttgataaaaa
    35761 actcaataaa actgtatcca atgaaaagtg tccttttgaa attatacaaa taggagcaat
    35821 taaattggat tctaaattca atataataga tacgtttaat agctatgtaa aacctacaat
    35881 ttataaagac atacatccct ttattagtcg tatgactaat attaaaagtt ctgattttaa
    35941 tgattcgcct actttccctg aggtctacaa caattttata aaatttatat catcacaaga
    36001 ccctatatta tgtgtttggg gagctggaga tttgaaggaa ttatacagga atatcaacta
    36061 tcacaagcta ccatcaaatt ccttacctaa atcttatatt aatatacaac aacatgcatc
    36121 taaatatttc aataatccag caggaaaaag cattggccta caaaatgcta tttctatatt
    36181 ggaacttgat gaaaaaatgt cttatcacaa tgctttaaat gatgcatatt atactgctaa
    36241 agtttttatt aagatataca atccaagtat agtcccagat atatatcttt atacgagtat
    36301 aaaacctaaa actataagat attctaacaa aaaaagagtt gattatgata agttatttga
    36361 tgaatttagg aaaattttaa atcgtgaatt aactaaagac gagaaaaaaa tcattaactt
    36421 agcttataat atgtggaaaa caaatcaatt tacattagaa aatgttaagc aaagaaaaaa
    36481 caaatagagt ttatttagat aaaaaatagg catgtagatt aaaaaactag ttaactttaa
    36541 atttatatct acagcccaat ttttatcata attaattttt attttatctc taattcaact
    36601 ggacaatggt ctgaacctaa tatttcagtg tgtatatccg cacttactaa tctatcttct
    36661 aattttttgg atgcacaaaa ataatctatt ctccaccctg cattattttt tcttgcatta
    36721 aatctatacg accaccaact ataaacacct tctttatcag gattaaaata tctatatgtg
    36781 tctataaacc cactgtctaa taatttagaa aacttctctc tttcttcttt agtaaaacct
    36841 gcatttttca tattattttt aggatttttt aaatctattt ctttatgtgc aacgttcata
    36901 tctccacaca caattacagg cttatgatta tctagtttgg ttaaataatc tataaagtcg
    36961 tcttcccatc tagttctata ttctagtctc tttaattcag attgagaatt tggcgtatat
    37021 actgtaacaa agtaaaaatc ttcaaactct agagttatca ctctaccctc tttgtcatgt
    37081 tcttctatat ttattccata cattactttt aaaggctctt tttttgtaaa tatagcagta
    37141 cctgaataac cctttctctc agcataattc caatattgaa aatatcctgg taaatctaac
    37201 tctatttgcc cttcttgcaa tttagtttct tgtaaacaaa atatatctgc atctacttct
    37261 ttgaaaaagt ctaaaaatcc tttcccaaca caagctctta ttccattaac attccatgat
    37321 ataaatttca tacttatact cctcatctaa acattgacac actccccata gctaaagatg
    37381 ggggcttttc aacaatttta ttgtaaattc ttaatttact tttattttaa caatgctata
    37441 ctataatttc atctaaaatt actatttact taatacctaa aaatatatgt tttaatcaat
    37501 ttttaacttt tttgttaaat cttcagggct cattgaagaa acatacatat ttgttccaag
    37561 ttcaaatcct ccaaaattat attttcttgg gattatgtgc atatgaacat taaaatacga
    37621 ttttgtttca atctttgttg gatgagtgtg aatacataaa ttaaatggca tatatccatt
    37681 gatattgtat aacttcttaa acaacttttc aaatattgta gaaagctcct gtatattatt
    37741 atcatttaac tcaccaaact ctattttatc tttaaatatt attcttactt ctccactata
    37801 cttggttgca tatggtataa gtaccaaaaa agcttctcca ttataaacaa ctcttctatc
    37861 taaacttatt tcattttcta ttaaatcatc atacaaagag cttttattgc tgtcataata
    37921 ctcctttgaa accaacagtt cattcgtaat ttcaggtgga ataaaagaca tagacaatat
    37981 ttgagcatgt ggatgcatca aagatgctcc tgcctttctc aagaagtttt tgaagatact
    38041 aacatactct actttatcat ctttcgacaa atctctaaat ctatttctat acataaaaaa
    38101 aacatcttca aattcttttt gactcatgtt ataaaaacta cgattatgtt tatcgctctc
    38161 tatcattact tcatgaacac catatacatc tcttgccata tcatcaatta taggaaattt
    38221 attgtagaca gattttacaa gccatccttc ttcactatct atttcaaata atgcatcagt
    38281 tgcatgagct tcatttcctt tgcaaaatgg gcattcttca tcatattcat tagtttcttc
    38341 atcttcattt gggatatctg ctgtatctaa aggtcttttt aacctatctg tagcaaatat
    38401 aacaacatca ttggtaattg gatctattct taattctttc ataattaaca cctcttagtt
    38461 aatagccttt aatataatta tatgatattt atcgtatctg ttaaataaat ttataatatt
    38521 ttttataatt ttgcatattt attattatat attttgtcta tagctgaaag attataacta
    38581 aaaaatcact atcagtacat cagaatatta tcttactata aattaattta tgaatacata
    38641 atttagtggc ataaacatgg gtattttact ataatttgtt aaaagtttat tttaaagcta
    38701 tattaatttt ttttgatgat ttttacccat aagtatgtta ttattatctt gtaaagatgt
    38761 cttgtattca aacatacaat ttacataata caatatacgt atttaaatta aatagggggt
    38821 attaaaatga attattcaaa cagagttagt gcaatgcaag cctctccaat cagaaaactt
    38881 gttccatttg cacaagcagc aaaagataaa ggaatcaaag tatatcactt aaacatagga
    38941 caaccagaca taaaaactcc taaaggattt tttgatgctg ttaaaaactt tgatagcgaa
    39001 gtcttagaat atgctacatc agaaggtatt ccagaattat tagaagcact tcaaaattac
    39061 tataaaactt acgatatgaa ttttgaaaaa gatgaattat tagtaactaa cggtggtagt
    39121 gaagctttac ttttcactat gatggcagtt tgtgacccag gagataatct attagttcca
    39181 gaaccattct atacaaacta caatggattt ggtcaatctg taaacgtaga agttaatgct
    39241 gttacaacaa aggcagaaaa cggtttccac cttccatcta aagaagaaat attgtctaaa
    39301 gtagatgata aaactaaggc tataattctt tctaacccag gaaatccaac tggtgcaatt
    39361 tatacaaaag aagaacttaa tatcctagct gaaatagcta aagaaaaaga tttatggata
    39421 atagcagacg aagtttatag agaatttgtt tatgatgggc ttgagtatac aagctgtggt
    39481 aacttagagg gtgtacagga tagagttata ataatagaca gtgtttctaa gagatacagt
    39541 gcttgtggtg ctagaattgg ttctatcgct tgcaaaaata aaggtcttat cgctcaaata
    39601 cttaaattat gtcaaggaag actttgtgtt cctacattag agcaaatagg agctgttgag
    39661 ttatacaaaa ctcctgtttc ttacttcaaa gaagttaatg aagagtacaa aaaaagaaga
    39721 gatgtattat acaatgaatt aatgaaggtt gaaggtgtta tatgtaaaaa accaactggt
    39781 gctttctata tagttgctaa attaccagtt gaaaatgcag aagatttcac aatttggatg
    39841 ttaaaagaat ttaataaaga taatgaaaca gttatggttt gccctgctga aggattctat
    39901 gctactccag gtttaggacg tgatgaaata agattagctt acatcttaaa tgaaaaagac
    39961 cttcatagag ctgctacatt attaaaagaa ggtttagagc aatatgtagc tttacaaaaa
    40021 taatttttta aacttaattt atataaattt aatgatataa aatgtcccct tttcagatat
    40081 tatttgaaaa ggggacattt cttaattaat atattaatta agacttagac ctgactattt
    40141 ttcattttct ttatatagtg ttggccagta atctttattc actttaatta aagcatctaa
    40201 aacttgtctt gccttagtta gagaaattat aaaaaagtac atcaagaaaa tttaagatgt
    40261 actttttaga ttgtatttag acttataatt atatagttaa ataaataaca aagaattaat
    40321 cttagtacca ttaaatacaa aaatttttat attttttcta tctaaattct tctttgttta
    40381 atatatattg aagagtattt attgcttcta aatatttttt tctgtcttct tcagttaatt
    40441 tagaaagttt ttctcctaaa ccatcactta atttatccat aaaatcagtt gctatcttat
    40501 ctccctcttc agtaatgtca attagatatt ttcttctatc aactaaatct cttttccttg
    40561 ttacatactt cttatcagtt aaatcatcaa ccatactagt taaactgcct ttttctatat
    40621 ttaacttaga gcataaatca gtcatactta ttactccata attttttatg aaaactaatg
    40681 ctcttaattg tgttttattt acattataat ctggtgcaaa tttttttagg tcttctaagt
    40741 acaggctaga ataaatctta ggaaatgttt ttactaaaaa acttatggta tctttta
//
LOCUS       DAELLV010000010        39077 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.010, whole genome shotgun sequence.
ACCESSION   DAELLV010000010 DAELLV010000000
VERSION     DAELLV010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 39077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39077
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.010"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            209..3757
                     /locus_tag="KPD58_000484"
     CDS             209..3757
                     /locus_tag="KPD58_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG0264271.1"
                     /translation="MMNRLKPRILGISILFIIIIILTCGAVFKFKFKLDNKLKETTHT
                     YLSEFTEHNIINLKTRLSGQFDMLESIANFIGNLNNINDEMVIDLMDSDVKRSSLIRM
                     TISTLDGECYSNDGITHNVQDRDYFKKAKNGEKNISEPLKSIVNNKEIIVLSTPVYKS
                     GKIVGVLSGIYDSKKLNELLGLSAFDEKADVFVAKKNGDIVAQKTNKNRKTLSCIFQL
                     FDKDNCDEDDCLKTIKENMKNDKSGYFNYNLDNEKFMLNYQPLGVNDWYVFSVVPNKI
                     VSIQSENISYDVYIFALEILLVILIFIIYIACIINNNTKIIINEKEKFKVLTDNILGG
                     VKTCLKDEYMTMTYVSQGFLELTGYSCEDLNKIFKNRFKDMIYKDDLDKFNCIILNQL
                     EKQDKIEVEYRIVRKDGSIIWILERSKSINDNKGSECIQGVLTDITELKKIQEELDSK
                     RFEIQAINDSIVGGIVITEINDDFDSIYLNEGYLNMIGYTRKQLEDELNNKLIKLIFK
                     DDRTELINSINKQLKLSDTFASEQRVEKRDGTIIWILLKGRKMIYDNGKSVLCCVIVD
                     ITSAKLLEQELKKSEERHEIILKQTQNIIIEWDIEKDKMNISPNWYNKFKYDPITENA
                     TKNIIKSVDIYHDDMDKFISLIKLTSQGSEYEECEIRLRTRDTIYAWFSIKATTLFDN
                     NGKPYKVVGSLTDIDESKREAEKLKERAEKDPLTDLYNKKASQSLIEEYITLDKKRKG
                     ALMIIDIDDFKGINDNLGHLYGDAVLSEIANDLINLFRATDIVGRIGGDEFIVFIKDI
                     SETRDIINKAEELVKTFERSFLGKGRNYKISLSVGIAKFPQDGMDYMTLFRNADIALY
                     SAKGKGKNCYTLYDKKFDKVQYITNTRNAGDSDNVEMSFKEHITNYIFDILYESSDIE
                     VAVNLILSIVGKHFNVSRMYIFENSDDNRVCKNTFEWCNEGVTSEINNLKNICYKDLG
                     NYLLNFNADGLFYCNDIENLGSDVKKVLKPQGIRSILQCKILEGNEFRGFIGFDECSY
                     NRMWTNDEVNTLTFISKTLSMFLLKMRIQSKLEESLTIQKSVMDNMDIWAYVVDRNTY
                     ELLFINKKMLESAPNTHVGDLCYKAIWNNQDKPCKKCPMIYLKDGDDTYKSFMHNKKF
                     EVQANITTTTLKWFDGKDACLIFGSEITSIEAEENT"
     gene            3942..5876
                     /locus_tag="KPD58_000485"
     CDS             3942..5876
                     /locus_tag="KPD58_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HBG0264272.1"
                     /translation="MENKFLPISKQDMIDRGWEELDFVLVTGDAYVDHHSFGTAIISR
                     VLESAGYKVGIIAQPDWRTTDDFMKLGKPRLAFLVNAGNMDSMVNHYSVSKKHREKDM
                     YSPGGKMGYRPDRATIVYCNKIREAYSDVAIVIGGIEASLRRFAHYDYWSDKVRKSML
                     IDSGADLLVYGMSEKQIVEVANALNDGYDPKYIRHIDGTCYISDTLEEIYDKYILIPS
                     YKEICEDKMKYVEAFKIQYDEQDPFRGNIIVQPHGSKYLVQNKPEKPLSREELDEVYG
                     LPYQKTYHPVYEKFGGIPAIEEVKFSIVSSRGCFGSCSFCAITFHQGRAVQSRSEKSI
                     IDEAIGITNLDDFKGYIHDVGGPTANFRRPACKKQITKGACKNRQCLSPSPCKNLDAD
                     HSEYLHLLRAVRKLPKIKKVFVRSGIRYDYVMADKNNKFLRELIEHHVSGQLKVAPEH
                     ISEEVLKYMQKPAGKTYDKFRQKFFAINEELGKKQYLIPYLMSSHPGSTLNSAIELAE
                     YLRDTHYQPEQVQDFYPTPGTLSTTMFYTGIDPLTMKPVYVPKSKRDKAMQRALLQYR
                     APRNYDLVYSALVEAGREDLIGFGHRCLIKPKNEKPYFNRNNSKKNVSKGTNKNKKTN
                     TNNRSNKNQQKSSTKKKKKH"
     gene            5982..6977
                     /locus_tag="KPD58_000486"
     CDS             5982..6977
                     /locus_tag="KPD58_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0264273.1"
                     /translation="MKKYISIILLVVLTMVVLVGCSPGKDNPKNKELSVVKQVKVAVP
                     DGLPAVAIAKLANENPEIKEGYETVYSIEKTPEAISTRVMKKDADIAIVPSNMAAIAY
                     NKTSSYNIVGTVGMGSLYLVSTENIKDYSDLVGKEVGCTGKGLTPDITIKSLLLQKDI
                     NYSDIKFNYVNSASELVPLLATGKVKTGIVPEPALSALMSKNPDIKIIKSLNDSWKEV
                     SGSKDGYPQSTLIVKSEFLRDNKDFVDSFVGQLSNSIDWANKNPEELGAYSEKIKIST
                     ESKIIGKSLERANLKYIPVKDMIKDYKNYYEKLANFDDKTLGGKVPDEAIYFVEK"
     gene            7005..7733
                     /locus_tag="KPD58_000487"
     CDS             7005..7733
                     /locus_tag="KPD58_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBG0264274.1"
                     /translation="MEVTLSCVILLFLWQIIALKIDNDIFLPTVGQVFNSIKEIMLNS
                     SFYIDILYSMGRCFFSFLLAMIFAIILSIVSYLNRFFRNLLKPINALTRSIPTMILVV
                     LALIWFEKDSTPFIVGFTIVFPILYDNVLGAILNIDKNLLEMANVYKIRFIDKVLKIY
                     LPAIKFQIISILVSTFSLSLKVAIAGEVYSQPTYGIGTMIQTEKINFNTSGIFAWIII
                     VLLISAILQIAQKFLARRAFLWKR"
     gene            7721..8419
                     /locus_tag="KPD58_000488"
     CDS             7721..8419
                     /locus_tag="KPD58_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0264275.1"
                     /translation="MEKIKLSIENINKRYDSRIIFRDFNIDFYVNEVNCILGKSGCGK
                     TTLLNIISGIIKNDTNNLNIKENLNRVGNKLEASYIFQDDRLIDWLTVEENIKIVVNK
                     YYNKTQLNKICDEYLELVGISDYKKFYPQMLSGGIRQRVNIARAFIYPSKNIIMDEPF
                     KSIDAKNTQLIMDNFRNILRKEKRTVLFVTHNIEEALFLADRIFILGDSPIRIKKILK
                     NSKELEKNEVLKLI"
     gene            8542..9015
                     /gene="ybaK"
                     /locus_tag="KPD58_000489"
     CDS             8542..9015
                     /gene="ybaK"
                     /locus_tag="KPD58_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HBG0264276.1"
                     /translation="MVKTNAMRILDSNKIDYKVMSYEVKSEHVDGVEVAHDIGRDVNE
                     VYKTLVTQGVSKNIYVYVIPVHENLDLKKAAKVAKEKSVEMIHVKDINKLTGYIRGGC
                     SPIGMKKLYKTFVNESAKNLDTIIVSAGKIGYQIELSPFDLQRLIKVEFVDVIKK"
     gene            9212..10051
                     /locus_tag="KPD58_000490"
     CDS             9212..10051
                     /locus_tag="KPD58_000490"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HBG0264277.1"
                     /translation="MRKIKSEQIVEQVKKLCIEASLYLGEDVLSCIKEKAKSEKSEVG
                     KNILNILVENAEIAKEKNIPICQDTGMAVFFVEIGQEVLIEGDTLTDAINEGVRQGYE
                     EGYLRKSVVSPINRVNTKDNTPAVIHYDMVKGDKIKIEFAAKGFGSENMSKMKMLKPS
                     DGLEGIKKFIIDTVSEAGPNPCPPMVIGVGIGGTVDKCAQIAKKALFRELGEFNKDEN
                     IAKLESELLTAINKLGIGPQGLGGTTTALGLNIETFPTHIAGLPVVVNINCHASRHKK
                     VVI"
     gene            10064..10612
                     /locus_tag="KPD58_000491"
     CDS             10064..10612
                     /locus_tag="KPD58_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HBG0264278.1"
                     /translation="MIKITTPVNEIDIAKLNCGDTISLSGILYTARDAAHKRLIDCIN
                     KGEELPFDVYGQGIYYVGPTPTKPGEVIGAAGPTTSYRMDDLTIPLLERGLRLMIGKG
                     KRSDEVIEGMQKYGAVYLAAIGGAGAYISNSIKSCEIIAYEDLGAEAIRKIVVEDLKL
                     TVAIDSYGNNIYEQGRAIYECK"
     gene            10678..11859
                     /locus_tag="KPD58_000492"
     CDS             10678..11859
                     /locus_tag="KPD58_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HBG0264279.1"
                     /translation="MSKDYAKLALEMHEINKGKVSVESKVEVKTKDDLSTAYTPGVAE
                     PCLKIHENQDDVYRYTSKGNLVAVVSDGSAVLGLGNIGAEASIPVMEGKAILFKQFAD
                     VDAFPICLKTNDVDEIVKSVELMEPVFGGINLEDISSPRCFEIEERLKKSLSIPVFHD
                     DQHGTAIIVAAAIINSIKLIENKKIEDLEIVINGAGAAGIAIAKILLNMNVKNIILCD
                     RSGALEASIENLNYVQKEMLKVTNIRNEKGPLKDIIKGKDVFIGVSGPGAVTKEMVES
                     MSEKPIILAMANPTPEIMPEEAKLGGAFIMGTGRSDFPNQVNNVLAFPGIFRGALDVK
                     AKEINEEMKIAAAYAIANTISDEEICPEYILPDVFNKNVVKNVAKAVKEAAIKTKVNR
                     I"
     gene            12154..12540
                     /locus_tag="KPD58_000493"
     CDS             12154..12540
                     /locus_tag="KPD58_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0264280.1"
                     /translation="MEYKVNDQELNASMFIPFVNRIWPGDYDMDKTQTALSKTLNITA
                     YDDKVLVGCLRVLSDGYYFGTITELLVLPEYQKQGIGSKLFQLAKENTPTMLYFGAQP
                     GVEEFYEKNGCQRSLQSYIIKKKDSY"
     gene            12690..12953
                     /locus_tag="KPD58_000494"
     CDS             12690..12953
                     /locus_tag="KPD58_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG0264281.1"
                     /translation="MEETKFCQCCAMPMGQTDELYGTNKDGSKSTDYCSYCFKDGEFT
                     ADISMEEMIEVCIPHMLQSNKDMTEDEARTMMNNFFPTLKRWK"
     gene            complement(13082..13924)
                     /locus_tag="KPD58_000495"
     CDS             complement(13082..13924)
                     /locus_tag="KPD58_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG0264282.1"
                     /translation="MINIGDFNKLTVKRKTEFGYFLDGQTNNTKDDILLHNRLIGKNE
                     INIGDEVNAFIFKDSDDRTAATLIPPLAKVGDVAHLKVVDNTDIGTFIDMGLPKDILV
                     PFKAKTYPLFRDEKYLFYIYLDKSKRIAATTDIDSYLLTDHTYNVGDIVTGVVYGFQT
                     NNSAMICVDNKYAGVILHNEYFTELKTGDVLENLHVIKIYEDGKLGLSPRGNRKDELD
                     TLENKILSYLEGSDGYMRFNDKSDPKDISILFNSSKKNFKRALGVLMKKGLIYQDEEG
                     TYLK"
     gene            14219..14953
                     /locus_tag="KPD58_000496"
     CDS             14219..14953
                     /locus_tag="KPD58_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0264283.1"
                     /translation="MKIVNKSSNIPLHTQLSSIIREMIETGELKEGDAIMPERELCNI
                     QNVSRMTVNKTIVGLVTEGLLYRVQGKGTFVAKQKKKYQFSNVKGFTDVMKEKGVNIK
                     TDILSFEMELPDDLVKRKLGISDNTTNIYKIVRLRYTDGEPFGLEIVYLSEEMCKGLT
                     KGILDNSSLYRVLNEKYGYKIQKAEQVMEPVILSDEESKLLETDEGALALKLHRNSYN
                     REGSPIEYTISIFRTDKYQYEIVLSE"
     gene            14963..16096
                     /gene="nagA"
                     /locus_tag="KPD58_000497"
     CDS             14963..16096
                     /gene="nagA"
                     /locus_tag="KPD58_000497"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBG0264284.1"
                     /translation="MKCLINGKIILKNQILENKVLVFDEKIIDIADSVPKDCEVIDAD
                     GKYISPGLIDIHIHGNMGKDTMDSTDESIETISKSIMRHGVTSFLPTTMTMDKEHVYD
                     ALEVIKKAQNRKLEGAQVLGAHLEGPFINENYKGAQNEKFIINSKYEFIKEYKDVIKV
                     ITYAPEKDIDFDFTREIKRCTDIVLSIGHSNANYDQAKEAINLGVTNVTHMFNAMTGL
                     NHRDPGVVGAALTTNVYSELIADTIHINKDLFQFILNNKGKERLILITDSIEAGGLED
                     GNYSLGGQKVIVKGNEARLENGALAGSVLSLNKMVFNFLDNTNLKVNEAINLASLNPA
                     TSLGINDKKGSLEIGKDADIAVFDENLDCKMTLCLGDVVYKNI"
     gene            16195..16944
                     /gene="nagB"
                     /locus_tag="KPD58_000498"
     CDS             16195..16944
                     /gene="nagB"
                     /locus_tag="KPD58_000498"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBG0264285.1"
                     /translation="MRVLVCKDYDGMSKKAAEMIAAQIVLKPNSILGLATGSTPVGMY
                     RDLVKKYNDNIVDFSDVMSFNLDEYYKLPISNDQSYDYFMKENLFNHVNIKPENTHLP
                     NGMADDIEKECMNYEASIDAAGGIDVQVLGIGRNAHIGFNEPDTKFAKRTHVVELTES
                     TIEANARFFKSREDVPKKAVSMGIGSILKSKKILLLASGEEKADAVYNTVYGDITPEV
                     PGSILQLHKDTIVIVDEAAASKLNPKDYKLV"
     gene            17119..17391
                     /locus_tag="KPD58_000499"
     CDS             17119..17391
                     /locus_tag="KPD58_000499"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264286.1"
                     /translation="MLKIIMFSGILSSIIKLPIIGILPTGNIKKNTGISDSKKDGKQR
                     RSNLGISIYQLPSFLYSLINNSVPSNKPIPPEEIKLIKMYLLKSCI"
     gene            18051..18755
                     /locus_tag="KPD58_000500"
     CDS             18051..18755
                     /locus_tag="KPD58_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0264287.1"
                     /translation="MSKLIYIADDEDNIRNLVKTFLKNEGHDVMDFKTGDELLEQFNI
                     KECDLVILDIMMPGSSGFEVCTKLREKSTVPIIMLTARDTDIDYITGITLGSDDYFTK
                     PFSPMSLVMRVKSIFRRIEFEKKQNYDKYSNSIDMELKFGDVIINKKNKIVTSKNVNI
                     DLTPNEYNLLTYLFENIDRAVSRDELLNKIWGYDIEVETRAADDTVKRLRKKILNTNI
                     LIETVWGFGFRLKEKS"
     gene            18757..20244
                     /locus_tag="KPD58_000501"
     CDS             18757..20244
                     /locus_tag="KPD58_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0264288.1"
                     /translation="MKNGNDKNRIGKIKSKFKLKFSKKNISIKTRILLSILCLIIIVF
                     MIILVSFNVFLDTYIKTTANEELTKSTEIVEHMDSNFRPIKPPQDDGKLPPKVLSNFM
                     RNVQDKVRMAETQSDADAMVVDSKYNLIFPTREDDFLKNVDEMELIRECIQNEKLDLN
                     SDENTRISTRNKDYYISTVKITSIYGEQDKYLILFIDISKTLNLAQKINIVLISVMCF
                     AGILAIFTAVILSEKIAKPIKELCEFAKKMGQGDFKRTYFDFSDKELVELSTVMNKSA
                     EYLDKYDNEQKIFFQNASHELRTPLMSIKGYAEAIKYNVIDSKHASDIILEESDRLSD
                     MVEDLLYISKIDNITKDFELVECDLREVLSNCGARQNVRAINKGIKFIYEFDEEMVLF
                     ECDEKNISKAFMNLIENALRYAKSEIKIVCKYNQKNIVVIIEDDGIGIKKEDLPHVFE
                     RFYKGVGGNHGIGLSIVKSIVNKHGGRIYVENGKKGAKFTILFKL"
     gene            20449..22062
                     /locus_tag="KPD58_000502"
     CDS             20449..22062
                     /locus_tag="KPD58_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG0264289.1"
                     /translation="MKEQLIFNITNLVTVVFEAFVIHMFLSDFLGQKEGYIKVVRYAK
                     LGFIICLGFCNLITLNPKITMPLIFILIFSTSFLYKGNLKTRLFTTVLLSIFFILSEI
                     VVTSIFVLFVKEGFEIMLENNSIRVLATILSKIVFLLTCKIICLFKKDVHLDMPIKYW
                     LPLFLIPIFSLFLSVSIFDVSKFFSLESLKFLSLISSVGILYINFIVFYLFKFIIDKT
                     KLSMKYELLEKEIIHKEELRLSNECYKIIVEQTDSVVFEWNIKENKSFVSQAWTEKFG
                     YNNACKNIFKEIKDKDLVHSEDKAIFEGFLESIKKKNMHNQAVYRLKKSNGEYIWCRT
                     SITSIYNDENELLRVVGVIVDVDSDIKKYEELRTRAESDSLTNIYNKGTFEKLVEETI
                     VMNTGDKKDALFIIDLDDFKEINDNFGHPFGDFVLKTFADKIQTSFGSKDLVGRIGGD
                     EFVVYMQDYVTEVNLHKKAKELNRVLSDNYTDLSFSFDASVSIGIARYPQDGTSFFEL
                     FKNADRALYSIKASGKNSYCLFEEELYVQ"
     gene            complement(22425..23255)
                     /locus_tag="KPD58_000503"
     CDS             complement(22425..23255)
                     /locus_tag="KPD58_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0264290.1"
                     /translation="MSQVSKNYFTTGEFAKICGINKKTLFHYDDIGLFSPELKKENGY
                     RYYSYHQLSIFGIISSLREVKMPLKEIKAYIDKRTPNLLIELLEKKTIDIKNEIEKLN
                     NIQALMESTISFTKSACNIDANTITLKEHEEEYLVKTPVTYKEQFLGDEEENFLYECI
                     NFMDNYELSDYGTIGSIIKGEDIINKDFESYSYLFTKVNKEYKKYPVSIKPKGLYVTA
                     YHKGSYETIYKAYEKLLNFFNQNNLRIGDFVYEEYLLYDISVRDSNEYLTQISAEVKM
                     "
     gene            23328..25055
                     /locus_tag="KPD58_000504"
     CDS             23328..25055
                     /locus_tag="KPD58_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0264291.1"
                     /translation="MKKLIHFLKPYRVLIVVVLIFTFLQTLGTLYIPTLTANIVNNGV
                     VKGDIDYIVKTGLMMMIVAGITALSAVLVCKVSANLSSGFCRDIREAVFIKSQDLSIN
                     DFNNIGTASMITRSTSDITLIGQSVFMFIQLVLPAPIITVSGLFLAYSIDKAMTIIIV
                     VVMFLFMLSAFLVGKKLIKLFKMMQIKMDNMNRVLREVVTGVRVIRAFNRSRFEKKRF
                     DRTAIDYSETAISINKIFAVLMPIVMLIMNLGIVSIIWFGGMRVSNGNMEIGHIMALV
                     EYCILILFYLIMGVMVFMYIPRAGACADRVNQILDIEPEIVDGNGHKDTVSERGHLVF
                     KNVTFSYAQSEEPVLNNITFEAKSGEVTAIIGSTGSGKSTIANIIPRFFEIQSGEISI
                     NGQDIKKIPQKELRDKIGFVPQKAFLFSGTIEENIRYGKEDASIEEVKHAASIAQADE
                     FIYDMEDKYDSFVAQGGNNLSGGQKQRISIARALVRKPEVYVFDDSFSALDFKTDKRL
                     RKALKNEIKDSSAIIIAQRISTIMDANQIIVLNDGKIVGIGKHKDLLENCEVYKQIAD
                     SQLSKEELA"
     gene            25092..26954
                     /locus_tag="KPD58_000505"
     CDS             25092..26954
                     /locus_tag="KPD58_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0264292.1"
                     /translation="MSEKRIDEQDIEVLSFEDGMSAEKAKDAKKTTKRLLKYMAKQKF
                     KILIIFISVLISSALTVLAPMVMGKAIDQLFNGIKTAVQTGTKFSVNFSTMGGIVSIL
                     LGLYLISAVFIYIQQYIMSGVAQNLVLSMRKDLSDKLNKLPLKYYDSHKKGETLSIVT
                     NDLEKVADSLQEGLMQLITAVVTVVGSIVMMISISVPLTIVSAITLLVSLGITVVIAR
                     KSQERFSENQKALGELNSNIEEIFTGQIVVKAFSKEKDTIRNFKILNQNLYNASRKAQ
                     FSSYAISPIIRFINLIGYVIIAVVGGIFASTGAMTLGSIQAFIQYVNQASEPTTEISY
                     IVNMLQAAIASAERVFTVMDEVEEIKDEESSKVISMPKGKVQFEHVKFGYSDDFILMK
                     DININLNAGDKIAIVGPTGAGKTTLVNLLMRFYEIQGGRITIDGVNIKDLKRGELRTM
                     FGMVLQDTWLFNGSIKENIAYSKSDATMDEIVRAAKSARVDHFIRTLPQGYDTILTED
                     ASNLSQGQKQLLTIARAILSDPSVLILDEATSSVDTRTEVEIQKAMNNLMKGRTSFVI
                     AHRLSTIRDADLILVMKEGTIIEQGSHKELIEKKGFYEELYNSQFTSEYDEDVV"
     gene            27287..27976
                     /locus_tag="KPD58_000506"
     CDS             27287..27976
                     /locus_tag="KPD58_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HBG0264293.1"
                     /translation="MNKKLNYRFEMKHKITEADVLALKSRLYPIMKKDENASKDGKYL
                     IRSLYFDTPEDKALLDKLNGVAIREKFRIRFYNNDCSYIRLEKKIKHYNMTSKLSTNL
                     TKKEVINILNNDIEFLKESTNCLLREFYLKLKCERLEAKSIVDYNREAFIYPVGNVRV
                     TIDSDIKTSVNSVDLFNKDLPTVSVIDENMTVLEVKYDEFIPDFIKDLIQINKTTSTA
                     VSKYASSRLYI"
     gene            28072..28758
                     /locus_tag="KPD58_000507"
     CDS             28072..28758
                     /locus_tag="KPD58_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HBG0264294.1"
                     /translation="MILIMTFNDIFKSSFIENVSGFSFVDSALALGSAFLVGLFIYMV
                     YKKTYMGIMYSRPFNVSLVALTMLTTFVILAVTSNVVLSLGMVGALSIVRFRTAIKDP
                     MDLVFLFWSLGSGIVLGAGLIPLAVMGSIIMGLILIFFSNKTISETPYILMVNCNNED
                     SEDIATDKIKKVFTKYQIKSKSVTPEKGIELVFEVRMKDGETSLINDLSQVDGVTNAV
                     LVSYNGDYVA"
     gene            28775..30682
                     /locus_tag="KPD58_000508"
     CDS             28775..30682
                     /locus_tag="KPD58_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HBG0264295.1"
                     /translation="MKDKKFTLLISIMIIFLCAVVGVYSTSSNKSVDLYSDVYIEKYF
                     NRDKVMEVNIEIDESDLKDMNENAIKEEFKVAKVTVDGDTYGNVGIRTKGNSSLISVA
                     NSDSDRYSYKINFDKYNTSQSMEGLTQLNLNNCYSDPSYMREFLTYSICEEMGLATPE
                     FAYAKVSINGEYHGLYLAVEGLKESYLENNFGNVTGDLYKSDEGSSLQYKGDDPESYS
                     NLIVESDKKTADWSKITKLLKSLDTGEDIEKYLDVDSVLKNIAINTALLNLDSYQGSF
                     AHNYYLYEQDGVFSMLPWDFNMSFGGFSGFGGGSQSIAIDEPTTGNLEDRPLISSLLK
                     NETYKTKYHKYLEEIVTKYLDSDYLENMTTKLHDMIASYVKEDPTAFYTYEEFEKNIT
                     SSIEDSSDNKGFGNKGFDNNNSNNSDSNNNSNSENKHSGNQSDEKEVNAELTSSVVKA
                     NTDNENKNKTTNDSESKNNTDKDKSGNDNNQKLEGPMGKGGKSIPGVLEVAEDMSKTI
                     KSQLSGETSSTKQNSGDESSSGIKGSEKFDEDMSGMPEPPEGMDGKMPPGMGNMDKGD
                     MNGKNGNMNMDRNQDNPREAGGFGNRGGGSVSKTTTYFKLILGGASMIIMSIMLVGVS
                     RVKRRRFIKSK"
     gene            31134..31823
                     /locus_tag="KPD58_000509"
     CDS             31134..31823
                     /locus_tag="KPD58_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264296.1"
                     /translation="MLQKVLWKFLKLVVGLFICSVGIVLTINCNLGLSPWDVFHQGLS
                     NHIGITIGTASIIVGSIVVIADVVLGENVGWGTVFNMLLIGFFMDLLLYSNLIPEADN
                     LFVGIIMLILGLVLLSVGMVFYMGSGLGSGPRDGLMVAIQKKTGKSLKLIRGTIEVGA
                     LIVGFLLGGKVGIGTIISAFGLGYFTQMVFGLFKLDCSKIKHRFIVDDIKFIKVYMSG
                     EKKAAVQNIVK"
     gene            32259..32798
                     /locus_tag="KPD58_000510"
     CDS             32259..32798
                     /locus_tag="KPD58_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0264297.1"
                     /translation="MDIGEKIKRLRTEKQLTQEELANRCELSKGFISQLENNLTSPSI
                     ATLIDILEILGTNLREFFNEIDDERISFTKEDMFETEDEDLKYKLKWLIPNSQKNEME
                     PIIITLYPGGQYKEEKPHEGEEFGYVLAGSIYVHIGEKKNKVKKGESFYFRPKANHYI
                     SNEGKTTAKVIWVSTPPSF"
     gene            32817..33860
                     /locus_tag="KPD58_000511"
     CDS             32817..33860
                     /locus_tag="KPD58_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0264298.1"
                     /translation="MVENIIELRDISKHYEELTVLDNFNLDIKKNEFLTLLGPSGCGK
                     TTTLKIIAGFEYADDGKVLFEGKEINNLPPYERQVNTVFQKYALFPHMDVYENVAFGL
                     KIKKTPKDVIDAKVKEVLKLVALEGFERRQVESLSGGQQQRIAIARALVNEPKVLLLD
                     EPLGALDLKLRQEMQIELKRIQKKLGITFVFVTHDQEEALTMSDTIVVMYKGKIQQMG
                     TPQDIYNEPKNSFVAKFIGESNIFDGIMIEDYKVNFCNRDFECVDKGFEKNENIEVVI
                     RPEDIKMVKPEEGMLKGKVTSTVFKGVHYEIELNENGRLWILHNTKNAEVGTELGMDI
                     YPEDIHIMRKENN"
     gene            33862..34698
                     /locus_tag="KPD58_000512"
     CDS             33862..34698
                     /locus_tag="KPD58_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0264299.1"
                     /translation="MKRKSFLAYPYVVWSAIFVIIPLILVVFFSFTKESGGGYAFTLE
                     NYKEVIDPIYMKVFGRSILLAGGATLICLIVGYPVAYIISKARVSRRGSLILLFILPM
                     WMNFLLRTYAWVAILGKNGLLNTFLGWFGIQPLAILYTNFAILLGMVYNFLPFMVLPI
                     YTALSKMDNDLINAAHDLGANNMTVFRKIIFPLSLPGVMSGITMVFMPAVTTFAISRL
                     LGGGKIMLVGDLIEQQFTVVGDWNFGSAISIFMMIVILISMSIMSKFGDESDKEGGGL
                     LF"
     gene            34803..35477
                     /locus_tag="KPD58_000513"
     CDS             34803..35477
                     /locus_tag="KPD58_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0264300.1"
                     /translation="MARWNGFTWKWYGQLLQNESIMSALYYTIVIAILASVISTIVGT
                     ISAIGIHKMRGKSKKLILNVNYLPILNTEIVTAVALMSLFVFVKMEFGFTTMLLAHIM
                     FCLPYVILSVLPKMKQLPDNIEDAAMDLGATPIYALRKVILPQIKPGIVSGFLIAFTM
                     SIDDFIISFFNAGNGVSNLSIEIYGMARRGIKPEINALSTIMFAVVLGLLLLANKKES
                     IVRGIK"
     gene            35477..36529
                     /locus_tag="KPD58_000514"
     CDS             35477..36529
                     /locus_tag="KPD58_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG0264301.1"
                     /translation="MIKFKKAISLIAVGMMTVTLFTGCNKATDSTKVLNVYNVGDYID
                     ESLIDKFEKETGIDVQYSTYDTNEMMYQKVKSGSTHYDLVFPSDYMVEKMKNEGLLEK
                     LDFKNIPNMKYLDKSFLNPVYDKTNEYSVAYMWGTLGILYNKKEVKDPMDSWNILWNP
                     KYKGNIMMFDSVRDTIGITLKKLGYSMNSVNPKEINEAKNLLMKQKDLVLAYVNDEGK
                     DRLLGGEVAMGMLYSGDAVTLMEQNPDLDYAIPKEGTNKWVDAMCVPTTAQNKKEAEL
                     FINFLLDPENAKVNAEYIGYSTPNTGALKLLDPEITQNPVAYPSKEVLDKCETFEDIG
                     ENIKLYDKAWIELKSK"
     gene            36759..37607
                     /locus_tag="KPD58_000515"
     CDS             36759..37607
                     /locus_tag="KPD58_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG0264302.1"
                     /translation="MNRINKKIDLLILALLVFIFLIVGIVILSIRTENKLNVFIMLAI
                     IFFIIMLTYLSNSVVGLITSSIIIFMYTSYILYNNITHNMDVEFISYMWIIATPVSSI
                     IMGNLNKSINELQNTNKKLSEQYKELVTIDSETGLRNLKIFYNDVNMEISKSIRHNTD
                     FSLMIVKLPYYGNLQTIFGENKTNKIVKYIGSNIIECTRNEDIIYSLQKDMIGILMPN
                     TSLEGSKVVKDRIKKRIKELNLDLNNRGKYVNIDVKIAFLQYKSSFGDSINFKNIVEE
                     ELQYDV"
     gene            37597..>39077
                     /locus_tag="KPD58_000516"
     CDS             37597..>39077
                     /locus_tag="KPD58_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG0264303.1"
                     /translation="MMFKIFNKSLLKLVVVLSIVLTISNFSLSSYASEITSSTDKVLI
                     IYDSKKETAYNRDILNIMRTLLGRFSSDIELLKLSNYDGEINKNYYSHIFILGINENS
                     YNNDKNTKNLISSLNSYKGTICWLGYGIENLLEHKKYNLDYVGKTNNIVSVNYRGKSY
                     NLDEHYVFNIVESKDTSNKVIGSINDTLNKYPYIINDKNLFYVSKLDLDGVLFYIFCD
                     SLNDIFNIKTFDKGRIFVRIEDVHAFREPKNLVEIADYLSSKNIPFTIALIPAYVNPK
                     NHKVITLSESPEIVKAIKYMQDKGGTVILHGYTHQYKKEEVSGEGYEFWDGKKDEPLK
                     ENMKIFVKDRVLNGLRVCIENGIYPLAFEAPHYAMESEGYKELKKYFSTYIGQHQNND
                     KKFSTNTYPYIIRDTEEFNIFIPENLGYIDPEDKFTFQNIKENLDKLSIVRGFSGGFF
                     FHSYLNIEYLKNTIEYLEKQNIEFMNLRDFNNWVKVDEIQIRN"
BASE COUNT        15463 a         4017 c         6825 g        12772 t
ORIGIN      
        1 aataaagtta taatttttaa tattaattaa gaatgatgaa atatgaataa agattttaca
       61 aataaatctt aagctatctt atttaattct aatacaggaa gaaggtaaat gtatattgaa
      121 aaaatcatag aaatagaagt aaacaattta aataaataca aaggtttgca aaagaagtaa
      181 tataattata tacaatgagg atgtgagtat gatgaacaga ctaaagccaa gaattttagg
      241 tattagtatc ttgtttatta ttataattat tttaacatgt ggagctgttt tcaagtttaa
      301 atttaaatta gataataaat taaaagaaac cacacataca tatttgtctg agtttacaga
      361 acacaatata attaatctca agacaagact tagtggtcaa tttgacatgc ttgaatctat
      421 tgctaacttc ataggaaact tgaataatat aaatgatgaa atggtgattg atttaatgga
      481 ttctgatgta aagcgaagtt ctttaattag aatgactatc tctacattag atggggaatg
      541 ttattctaat gatggaatta cccataatgt tcaagataga gattatttta aaaaagctaa
      601 aaatggagaa aagaatatat cagagccttt aaaatctatt gttaataata aagaaataat
      661 agttttatct accccagttt ataaaagtgg gaaaatagtt ggtgttttaa gtggaattta
      721 tgattcaaaa aagttaaatg agttattagg attatctgca tttgatgaaa aagcagatgt
      781 ttttgttgca aagaaaaatg gagatatagt tgctcaaaaa acaaataaaa atagaaagac
      841 gctatcatgt atatttcagc tttttgacaa agataattgt gatgaagatg attgtctaaa
      901 aactataaaa gaaaatatga aaaatgataa gtctggatat tttaattata acttagataa
      961 tgaaaaattt atgttaaact atcagccact tggagttaat gattggtatg ttttttctgt
     1021 tgtgccaaat aaaatagtat ctattcagtc agagaatata tcttatgatg tatatatttt
     1081 tgcattagaa atactactag ttatattgat ttttataata tatatagcat gtattataaa
     1141 taataacacc aaaattatta taaatgaaaa agaaaaattt aaagtactca ctgataatat
     1201 attaggaggg gttaaaacct gtttaaaaga tgaatatatg actatgacat atgtaagtca
     1261 ggggttttta gagcttacag gttactcttg tgaagattta aataaaatat ttaaaaatag
     1321 atttaaagac atgatttata aagatgattt agacaagttt aattgtatta ttctaaatca
     1381 attagaaaaa caagataaaa ttgaagttga atatagaatt gtaagaaaag atggaagtat
     1441 aatttggatt ttggagagaa gtaagtctat aaatgataat aaaggaagtg agtgtattca
     1501 gggagtttta actgatataa cagaacttaa gaaaatacaa gaagaattag attcaaaaag
     1561 atttgaaata caggctatca atgatagtat tgttggtgga atagtgataa ctgagataaa
     1621 tgatgatttt gatagtatat atttaaatga aggttattta aatatgattg gatatacacg
     1681 caaacaactg gaagatgagc taaataataa attaatcaaa cttattttta aagatgatag
     1741 aacagaatta attaatagta taaacaaaca actcaaatta agtgatacat ttgcatctga
     1801 acaaagagtt gaaaaaaggg atggcactat tatatggatt ttattaaaag gaagaaagat
     1861 gatttatgat aatggaaaat ctgtactttg ttgtgttatt gtagatatta cttctgctaa
     1921 attattagag caagaactta aaaagagtga agaacgtcat gaaatcattc taaagcagac
     1981 gcaaaatatt ataatagaat gggatataga aaaagataaa atgaatatat cacctaattg
     2041 gtataataag tttaaatatg accctataac tgaaaacgca acaaaaaata taattaaatc
     2101 agtagatata tatcatgatg atatggataa gtttatttca ttaattaaac ttacttcaca
     2161 aggtagtgag tatgaagagt gtgaaattag acttagaact agagatacta tttacgcttg
     2221 gtttagtata aaggcaacaa ctttatttga taataatggt aaaccttata aagtagtagg
     2281 aagtttgact gatattgatg aatcaaaacg agaagctgaa aagttgaaag aaagggcaga
     2341 aaaagaccct cttacagatc tgtataataa aaaagcatca caatcactta tagaggagta
     2401 cataacattg gataaaaaaa gaaaaggtgc tttaatgatt attgatattg atgactttaa
     2461 aggaataaat gataatttag gacatttata tggggatgct gtattaagtg aaattgcaaa
     2521 tgacctaata aatctattta gagcaaccga tatagtaggc agaattggtg gagatgagtt
     2581 tattgtattt ataaaagata tttctgaaac aagagatatt ataaataaag cagaagaatt
     2641 ggtaaaaaca tttgaaaggt cttttttagg taaaggacga aattataaaa tatcattaag
     2701 tgtaggaatt gctaagtttc cacaagatgg tatggactat atgacactat ttagaaatgc
     2761 ggatatagct ctttactcag ccaaagggaa gggcaagaat tgttatacat tgtatgataa
     2821 aaaatttgat aaagtccaat atataactaa tacaagaaat gcaggagata gtgataatgt
     2881 agagatgtct tttaaagaac atatcacaaa ttatatattt gatatactat atgaatcaag
     2941 tgatatagaa gtggcagtaa atttaattct aagtattgta ggtaaacact ttaatgtgag
     3001 tcgcatgtat atatttgaaa attcagatga taatcgagta tgtaaaaata catttgaatg
     3061 gtgtaacgaa ggtgtaacct cggaaattaa taatcttaaa aatatttgtt acaaggattt
     3121 aggcaactat cttttaaatt ttaatgcaga tgggttattt tattgtaatg atattgaaaa
     3181 tttaggtagt gatgtgaaaa aagtacttaa gccacaaggc atacgttcaa tattacaatg
     3241 taaaatttta gagggtaatg aatttagagg gttcatagga tttgatgaat gttcatataa
     3301 tcgtatgtgg actaatgatg aagtgaacac tttgacattt atatctaaaa cgctcagtat
     3361 gtttttatta aaaatgcgta ttcagagtaa gctagaagag tctttaacaa ttcaaaaatc
     3421 tgtgatggat aatatggata tatgggcata tgttgttgac aggaatacct atgaattact
     3481 atttatcaat aaaaaaatgc ttgagtcagc accaaacact catgtaggtg atttatgtta
     3541 taaagcgata tggaataatc aagataaacc ttgtaaaaaa tgccctatga tatatttaaa
     3601 agatggtgat gatacttata aatcattcat gcataataaa aagtttgagg tacaggcaaa
     3661 tataactaca actactttga aatggtttga tggtaaagat gcatgtttaa tatttggaag
     3721 tgaaataact agtattgaag ctgaagaaaa tacttaaaat actgtgttat atatatactt
     3781 aaaatcgcaa tttgaattta ttagacttaa aatgtctata taaatcattt aagttgtggt
     3841 tttaagtatt ttttatgata taattaagcg ttaagaacaa aattaaaaga tacttttgta
     3901 taaaaaaata tttgttaaat atatattgag caggtgataa tatggaaaat aagtttttac
     3961 caataagcaa acaagatatg atagatagag gatgggaaga attagatttt gttctagtta
     4021 caggagatgc ttatgtggac catcatagtt ttggaacagc aataatatct agagttttag
     4081 aaagtgctgg gtataaagtt ggaataatag ctcaaccaga ctggagaact acagatgatt
     4141 ttatgaagct aggaaaacct agacttgcat ttctagttaa tgcaggaaat atggattcaa
     4201 tggtcaatca ttattctgta agtaaaaagc atagagaaaa ggatatgtat tctccaggtg
     4261 ggaaaatggg ttatagacca gatagagcta ctatagtata ttgcaataaa ataagagaag
     4321 catacagtga tgttgcaata gtaataggtg gaattgaagc gagcttaaga agatttgcac
     4381 actatgatta ttggagtgat aaggttagaa agtctatgtt aatagatagt ggagcagatt
     4441 tattagttta tggtatgagt gaaaagcaga ttgtagaagt agccaatgca cttaatgatg
     4501 gatatgaccc aaaatacatt agacatattg atgggacatg ctatatttca gatacattgg
     4561 aagaaatata tgataaatat atattaattc catcatataa agaaatttgt gaagataaaa
     4621 tgaagtatgt agaagctttt aaaattcaat atgatgagca agacccattt agaggtaata
     4681 taattgttca acctcatgga agtaagtatc tggttcaaaa taagccagaa aaaccactta
     4741 gtagagaaga actagatgaa gtatatggat taccatatca gaagacatac catcctgtgt
     4801 atgaaaaatt tggaggaata ccagctatag aagaagtaaa atttagtata gtaagttcaa
     4861 gaggctgttt tggtagttgt tcattttgtg caataacatt tcatcaaggt agagcagtgc
     4921 aaagtagaag tgaaaaatca attattgatg aagcaatagg aataacaaat ttagatgatt
     4981 ttaaagggta tatacatgat gttggaggtc caacagctaa ttttagaaga ccagcatgta
     5041 aaaaacaaat tactaaaggt gcttgtaaaa atagacaatg cttatctcca tcaccatgta
     5101 aaaatttaga tgcagaccac agtgaatact tacatttact tagagctgta agaaaattac
     5161 ctaaaataaa aaaggtgttt gtgcgttctg gaattagata cgattatgta atggcagata
     5221 agaacaataa atttcttaga gagttgatag agcatcatgt aagtggtcag ttaaaggtag
     5281 ccccagaaca tatatcagaa gaagttttaa agtatatgca aaaaccagca ggaaaaactt
     5341 atgataaatt tagacagaag ttttttgcta taaatgaaga attaggtaaa aagcaatatt
     5401 tgatacccta tttaatgtct agtcaccctg gttctacctt aaatagcgct atagagttag
     5461 cagagtattt aagagatact cactaccaac cagagcaggt tcaggatttt tatccaactc
     5521 cagggactct atctacaacc atgttttata caggtattga cccgcttaca atgaagccag
     5581 tttatgttcc taaatcaaaa cgtgacaagg cgatgcaaag ggctttatta caatatagag
     5641 cacctagaaa ttatgatttg gtatactctg cgttagttga agcaggaaga gaagacttaa
     5701 ttggttttgg acatagatgt cttataaagc caaaaaatga aaagccttat tttaatagaa
     5761 ataattctaa aaaaaatgta tctaaaggaa caaataaaaa caaaaaaact aatacaaata
     5821 acagaagtaa taaaaatcaa caaaaaagtt caactaaaaa aaagaaaaaa cattaaagta
     5881 taaataaaaa tacttaaaaa agaaggatta ttcgataaat taacagaaat tagtataaat
     5941 aaatataatt attttaatga cgttaccttt gggaggaaga gatgaaaaag tatatttcta
     6001 taatattatt agtggtgttg actatggttg ttttggtagg atgttcacca ggaaaagata
     6061 accctaaaaa taaggagcta tcagttgtca aacaagtaaa agtagctgtt ccagatggac
     6121 taccagcagt ggcaatagct aaacttgcaa atgaaaatcc agagattaaa gaaggatatg
     6181 aaacagtgta tagcattgaa aagacacctg aagcgatttc cactagagtg atgaaaaaag
     6241 atgcagatat agctatagtt ccttcaaaca tggctgcaat agcgtataat aaaacttcat
     6301 catataatat tgttggaact gtaggaatgg gttcacttta tttggtgtca acagaaaata
     6361 taaaagatta ttctgattta gttggtaaag aagttggatg tactggaaaa ggacttactc
     6421 cagacataac tataaaaagt cttttgttac aaaaggatat aaattattca gatataaaat
     6481 ttaattatgt aaattcagca agtgaactgg taccattatt ggctactggt aaagttaaaa
     6541 caggtattgt tccagaacct gcacttagtg ctttgatgtc taaaaatcca gacataaaaa
     6601 ttataaaaag tttaaatgac tcatggaagg aagtaagtgg ttcaaaagat ggttatcctc
     6661 aatctacttt aattgtaaag tctgaatttt taagagataa taaagatttt gttgattctt
     6721 ttgtaggtca attatcaaat agtatagatt gggcaaacaa aaatcctgaa gaattaggtg
     6781 catattctga aaaaataaaa atatctactg aatcaaagat aataggaaaa tctcttgaaa
     6841 gagctaattt aaaatatatt ccagttaagg acatgattaa agattataaa aattactatg
     6901 agaaattagc taattttgat gataagacat taggaggcaa agtaccagat gaagcaatat
     6961 actttgttga gaaataaaaa aataaagaac aatttataga taaaatggag gtaacacttt
     7021 cttgtgttat cctcttattt ttatggcaaa ttattgcttt aaaaattgat aatgatattt
     7081 ttttaccaac agtaggtcaa gtatttaata gtataaaaga gattatgcta aattctagtt
     7141 tctatataga tatactttac tctatgggga gatgtttttt tagtttttta ctggctatga
     7201 tttttgctat tatactttca atagtgtctt atttaaatag attttttaga aacttattaa
     7261 agccaatcaa tgctttgaca cgttcaatac caactatgat attggtagta cttgcactta
     7321 tttggtttga aaaggatagt acccctttta tagttggatt tactatagtt tttcctatat
     7381 tgtatgataa cgttttgggc gctatattga atatagacaa gaacttatta gaaatggcta
     7441 atgtgtataa aataaggttt atagataagg tgttaaaaat ttatttacca gccataaaat
     7501 ttcaaattat atctatacta gtatcaacat tctctttaag tttgaaagtt gctatagcag
     7561 gagaggtata tagtcaacct acatatggta ttggaactat gatacagact gaaaaaatta
     7621 actttaacac atctggaata tttgcatgga taattattgt tcttttaata tctgctattt
     7681 tacaaatagc tcaaaagttt ttagcaagga gagctttttt atggaaaaga taaagttgag
     7741 tattgaaaat ataaataaaa gatatgatag tcgtattata tttagagatt ttaatattga
     7801 tttttatgta aatgaggtaa attgtatcct tggaaaatct ggttgtggaa aaactacact
     7861 cttgaacata ataagtggga ttattaaaaa tgacaccaac aatctaaata tcaaagaaaa
     7921 tttaaataga gtaggaaata agcttgaagc tagttacata tttcaagatg atagacttat
     7981 agactggtta acagtagaag aaaatataaa gatagttgta aataaatact ataataaaac
     8041 tcaattaaat aaaatttgtg atgaatactt ggaattggtt ggaattagtg attataaaaa
     8101 attctatcct cagatgctta gtggaggtat tagacaaaga gtaaatattg ctagagcttt
     8161 tatatatcca tcaaaaaata ttataatgga tgaacctttt aaatctattg atgcaaagaa
     8221 tacacagctt ataatggata atttcagaaa tatattaaga aaagaaaaaa gaactgtgtt
     8281 atttgtaact cataatattg aggaagcatt gtttttagca gataggatat ttattttagg
     8341 agactctcca attagaataa aaaaaatatt aaaaaattct aaggaattgg aaaaaaatga
     8401 agttttgaag ttgatataga gttataataa tactgtcgtt ttaaaatata tttggttaaa
     8461 atctataaga ctatattaaa atagtacaga atattcaaaa ctgtgtataa tgttcgtatt
     8521 ttataaaaag gaagtgaatg tatggttaag actaatgcta tgagaatatt agattcaaat
     8581 aagattgatt ataaagtaat gtcttatgaa gtgaaaagtg aacatgtgga tggtgttgag
     8641 gtggcacatg atataggaag agatgtaaat gaagtgtata agacattagt aactcaagga
     8701 gttagtaaga atatatatgt atatgtaata ccagtacatg aaaacttaga cttaaaaaag
     8761 gctgcaaaag tagctaaaga gaaaagtgta gagatgattc atgtaaaaga tataaataag
     8821 cttacaggtt atattagagg cggatgttct cctataggta tgaaaaagct ttataaaact
     8881 tttgtaaatg aaagtgcaaa gaatttagat acaattattg tgagtgcagg aaaaatagga
     8941 tatcaaatag aactatctcc atttgattta caaagactta taaaagtaga atttgtagat
     9001 gtaataaaaa aataagaatt acatataaaa tatagagctt atattcttaa taataggtgt
     9061 tgtagtatta ataaaatgaa taataatttt aaaataaata ttcagttact tatgtgtaat
     9121 tggtataaaa atattgagaa attttaaaaa caatggtaaa gtgtaagtat aatcacgaat
     9181 attttttata aagtaatgga gggatgttcg cttgagaaaa ataaaatctg aacaaatcgt
     9241 cgagcaggta aaaaaacttt gtatagaagc gagcctatat ttaggagaag atgttttaag
     9301 ctgtataaaa gaaaaagcga aaagtgaaaa gagtgaagtt ggaaaaaata tattgaatat
     9361 attagttgaa aatgctgaaa tagctaaaga aaaaaatata ccaatatgcc aagatactgg
     9421 tatggctgta ttttttgttg aaataggaca agaagtttta attgaaggag acacacttac
     9481 tgatgctata aatgagggag taagacaagg gtatgaagaa ggctacttaa gaaaatctgt
     9541 tgtaagtcca attaacagag taaatacaaa agataataca cctgctgtta ttcattatga
     9601 tatggtaaaa ggtgataaaa ttaaaataga atttgcagct aagggatttg gaagcgaaaa
     9661 tatgagtaag atgaaaatgt taaagccatc agatggattg gaaggaatta aaaaattcat
     9721 aatagataca gtttcagaag ctggacctaa cccatgccct cctatggtaa ttggagttgg
     9781 tataggtggg actgtggaca aatgtgctca aatagctaaa aaggcacttt ttagagaact
     9841 aggtgaattt aataaggacg aaaatatagc aaaacttgaa agtgaactat taacagctat
     9901 taataaactt ggtataggac ctcaaggctt aggtggtact acaacagctt tgggattaaa
     9961 catagagact tttcctacac acatagcagg tttacctgtt gttgtaaata taaactgcca
    10021 tgcatcaaga cataagaagg tagtaattta ggaggaaaat aaaatgataa aaataactac
    10081 acctgtaaat gaaattgata tagcaaaatt aaattgtgga gatacaatta gtttgagtgg
    10141 tatactttat acagcaagag atgctgctca taaaagattg atagattgta taaataaagg
    10201 tgaagaatta ccatttgacg tatatggtca aggaatatat tatgtaggac ctactcctac
    10261 aaagccagga gaagttatag gtgctgcagg tccaacgacg agttatagaa tggatgattt
    10321 gactatacct cttttagaaa gaggtcttag attgatgata ggcaagggaa aaagaagtga
    10381 tgaagttata gaaggtatgc aaaaatacgg agcagtttat cttgctgcaa taggaggagc
    10441 tggagcatat atttcaaatt caataaaatc atgtgaaatt attgcatatg aagatttagg
    10501 agctgaagct ataagaaaga ttgtagtaga agaccttaag cttacagtag caatagatag
    10561 ctatggaaat aatatatatg agcaaggtcg agcaatttat gaatgtaaat aatgtttaga
    10621 aataaatatg aatatcaaaa ttaaaaatag ttgtgtatat tttaaggagg aaaattgatg
    10681 tctaaggatt atgcaaaatt ggcattagaa atgcatgaaa tcaacaaagg gaaagtaagt
    10741 gtagaaagca aggtagaagt taaaactaag gatgatttat caacagcata tacaccaggg
    10801 gttgctgaac cttgtttaaa aatacatgaa aatcaagatg atgtttatag atacacttct
    10861 aaagggaatc tagtagcagt agttagtgat ggaagtgctg tacttggatt aggtaacatt
    10921 ggtgctgaag catctatacc tgttatggaa ggtaaagcta tattatttaa acaatttgca
    10981 gatgtagacg cttttccaat atgtttaaag actaatgatg tagatgagat agttaaatct
    11041 gtagagttga tggagcctgt atttggaggt ataaatttgg aagacatatc ttctccaaga
    11101 tgttttgaga tagaagagag attaaaaaaa tctttaagta tacctgtttt tcatgatgac
    11161 caacatggga cagcaataat agttgcggca gccataataa actcaataaa gttaattgaa
    11221 aataaaaaaa tagaagattt agaaatagtt ataaacggag caggagctgc tggtatagcc
    11281 atagcaaaga tactacttaa tatgaatgtt aaaaatataa tcttatgtga tagaagtgga
    11341 gcattggaag ctagtataga aaatctaaac tatgttcaaa aagaaatgtt aaaagtaaca
    11401 aatataagaa atgaaaaagg acctttaaaa gatattataa aaggaaaaga tgtatttatt
    11461 ggtgtttcag gtccaggagc tgttacaaag gaaatggttg aaagtatgtc tgagaagcca
    11521 ataatattgg caatggcaaa tcctactcca gagataatgc cagaagaagc aaaattaggt
    11581 ggagcattta taatgggaac aggtcgttca gacttcccta atcaagtcaa taatgtactg
    11641 gcttttcctg gaatatttag aggtgcactt gatgttaagg ctaaagagat aaatgaagaa
    11701 atgaagatag ctgctgcata tgcaattgct aatacaataa gtgatgaaga aatatgccca
    11761 gagtatatat taccagatgt atttaataag aatgtagtaa aaaatgttgc taaggcagtt
    11821 aaagaagcag cgataaaaac caaagtaaat agaatataaa tttttatatg ttcatatatc
    11881 cttatattat cgggtatatg ggcattttta ttataggttt atactcatat atccttactt
    11941 ttatatacat atctttatga ttaaaggtgt tagaaaatgt agtaaatttt atactatcag
    12001 actctatctt tattacttta gtagatacat ggttgtaaag gtttatagaa aaaaatatag
    12061 gaaggcagat ttttattgtt gcgtatatat gagaatgata taatatgaat attataaatt
    12121 aataaattta aatttatgga ggtacaagag atgatggaat ataaagttaa tgaccaagaa
    12181 cttaatgctt caatgtttat cccattcgtc aacaggatat ggccaggaga ttatgatatg
    12241 gataaaacac agactgcatt atcaaaaact ttgaatataa ctgcttatga tgataaagtg
    12301 cttgtaggat gtctgcgagt tctttcagat ggttattatt ttggaacaat tacagaattg
    12361 cttgttcttc cagagtatca gaaacaaggc attggtagta agcttttcca gcttgctaaa
    12421 gagaacaccc caactatgct atattttggt gcacagccag gagtagaaga gttctatgaa
    12481 aaaaatgggt gccaaagaag tctgcaatcc tacataataa aaaagaaaga tagttattaa
    12541 agttccagct ttcaataatc ttaaaagtac aatattttag tggaagcatt tcatttttta
    12601 ttactaatat gacataaaca tgtcatatta gcattgatat acttttaata tggttaagag
    12661 ccaaagaaaa atataaggag tgatttaaaa tggaagaaac taaattttgc caatgctgtg
    12721 caatgccaat gggacaaact gatgaattat atgggacaaa taaagatgga agtaaaagta
    12781 ctgattattg tagttattgt tttaaagatg gtgagtttac agctgatata agtatggaag
    12841 agatgataga agtttgtatc cctcatatgc ttcaaagtaa taaggatatg actgaagatg
    12901 aagcaagaac aatgatgaac aatttctttc caacattaaa acgctggaaa taattttatg
    12961 caaaattagt tatatgcaaa acatgaaata tggtgttgag aataaggggt ggtgtgcttt
    13021 atagcatacc acctgttttg ttacttggag ttcagttatt ttcctaaata ttttaaatat
    13081 actattttag ataagttccc tcttcatctt gataaattaa tccttttttc ataagcactc
    13141 caagagctcg tttaaaattt ttcttacttg aattaaataa tatagaaata tctttaggat
    13201 cagatttatc attaaatctc atatatccat cagaaccttc taaataactt aaaatcttat
    13261 tttctaaagt atcaagttca tcctttctat ttcctctagg agaaagacca agttttccat
    13321 cttcatatat ttttatgaca tgtaaatttt caagaacatc accagttttt agttcagtaa
    13381 agtactcatt atgaagtatt acaccagcat atttattgtc tacacatatc attgcagaat
    13441 tattagtttg gaatccatat acaactccag ttacaatatc tcctacattg taagtatggt
    13501 ctgttaataa atatgaatct atatctgtag tagcagcaat tcttttgctt ttgtctaaat
    13561 atatataaaa taaatatttt tcgtctctaa atagtggata agtcttagct ttaaaaggaa
    13621 ctaatatgtc tttaggaagt cccatatcta taaaagtacc aatgtctgta ttatcaacta
    13681 cttttaaatg tgctacatcc ccaaccttag ctaaaggcgg tattaaagtt gcagcagttc
    13741 tgtcatcaga atctttaaat atgaaagcat ttacttcatc acctatgttt atttcatttt
    13801 ttccgattaa tctattatga agaagtatat cgtcttttgt gttattagtt tgtccatcca
    13861 aaaaatatcc aaattcagtt tttcttttta ctgtaagttt attaaaatca cctatgttta
    13921 tcaaattaaa atcatcctct cattttatat ttataattta gccataatat acttatgtag
    13981 tagtttgtca cagtatctat attagtatta gtgaaattta caataaataa tatagttatt
    14041 tatgtatttt tcaattctct agcttcttat atagtataaa ctccaatttc ttttttgtct
    14101 ttaggtttta ttaaaatttt tacaaaataa gttaaaaagt attgaaataa aatttctttt
    14161 aaagtataat tggtatatac caaaattggt atataccata tcgaaaagtg gtgactatat
    14221 gaaaatagta aataaatcta gtaatatacc attgcatact caattaagtt caataataag
    14281 agagatgata gaaactggtg agcttaaaga gggtgatgca ataatgccag aaagagaact
    14341 ttgcaacata caaaatgtaa gtcgtatgac agtaaataaa accattgttg gattagttac
    14401 agaaggactt ctttataggg tacaaggtaa agggacattc gttgcaaagc aaaaaaagaa
    14461 gtatcagttt tcaaatgtaa aaggttttac tgatgttatg aaagaaaagg gagtaaacat
    14521 aaaaacggat atattatcct ttgagatgga actaccagat gatttagtta aaagaaagct
    14581 tggaataagt gataatacta caaatatata taagattgtc agacttagat atactgatgg
    14641 agagccattt ggattagaaa tagtatatct atcagaagaa atgtgtaagg gtctaacaaa
    14701 agggatattg gataattctt cattatatag ggttttaaat gaaaaatatg gctataagat
    14761 acaaaaagct gaacaggtaa tggagccagt aatactttca gatgaagaaa gtaagcttct
    14821 tgaaacagat gaaggtgctt tagctcttaa acttcataga aattcctata atagagaagg
    14881 tagccctata gaatatacca tatcaatatt tagaacagat aaatatcaat atgaaatagt
    14941 attgagtgag taggtgaata atatgaaatg tttaattaat ggaaagataa tattaaaaaa
    15001 tcaaatctta gaaaataaag tattagtatt tgatgaaaaa ataattgata tagctgatag
    15061 tgtaccaaaa gattgtgaag ttatagatgc tgatggaaaa tatatatctc cagggcttat
    15121 tgatattcat atacatggaa acatgggaaa agatacaatg gattcgactg atgaatctat
    15181 tgaaacaatt tcaaagtcaa ttatgagaca tggagtaaca tcttttcttc caactacaat
    15241 gactatggat aaagaacatg tttatgatgc acttgaagtt attaaaaaag cccaaaatag
    15301 aaagcttgaa ggggcacaag tattaggagc acatttagaa ggacctttta taaatgagaa
    15361 ttataaggga gcacaaaatg aaaaatttat aataaattca aaatacgaat ttataaaaga
    15421 atataaagat gttataaaag ttataactta tgctccagaa aaagatattg attttgattt
    15481 tactagagaa ataaaaagat gtacagatat agttttatct attggtcata gtaatgcaaa
    15541 ttatgaccaa gctaaagaag ctataaattt aggagtaact aatgtaactc acatgttcaa
    15601 tgcaatgact ggtttaaacc atagagaccc aggcgttgtt ggtgcagcac ttacaactaa
    15661 tgtgtacagt gagttaatag cagatacaat ccatataaat aaagatttat ttcaatttat
    15721 actaaataat aaaggaaaag aaagactgat tttaataaca gattctatag aagctggtgg
    15781 tcttgaagat ggcaattatt ctcttggagg tcagaaagtc atagttaagg gtaatgaagc
    15841 tagacttgaa aatggagcat tagctggaag tgtgttgtct ttaaataaaa tggtatttaa
    15901 cttcttagat aatacaaacc ttaaagtgaa tgaagcaatt aatttagctt ctttaaatcc
    15961 agcaacatct ttgggaataa atgacaaaaa aggaagctta gaaataggta aggatgcaga
    16021 tatagctgta tttgatgaga atttagattg taaaatgaca ctttgcttgg gggacgtagt
    16081 atataaaaat atatagtata caaaacaaac cttaaagaca gtaaaaacaa tataaaaaat
    16141 aataaattaa acttacatta gtaaaagtag tagttaaaat ttaggaggaa taagatgaga
    16201 gtattagtgt gtaaagatta tgatggaatg agtaaaaaag ccgctgaaat gatagcagct
    16261 caaatagttt taaaaccaaa ttcaatactg ggactagcta caggaagtac accagtaggt
    16321 atgtatagag atttagtaaa aaaatacaat gataatatag tagatttttc agatgtgatg
    16381 agctttaatt tagatgaata ttataagtta ccaatatcta atgaccaaag ctatgattat
    16441 tttatgaaag agaatctatt taatcatgta aacataaagc cagaaaatac tcatctacca
    16501 aatggaatgg ctgatgatat tgaaaaagag tgtatgaact atgaagcttc tatagatgca
    16561 gctggtggta tagatgtaca agtacttgga ataggtcgta atgctcatat aggttttaat
    16621 gaacctgata ctaaatttgc aaagagaact catgtagttg aacttacaga gtctacaata
    16681 gaagctaatg caagattctt taaatcaaga gaagatgttc ctaaaaaagc tgtaagtatg
    16741 ggaataggtt caatattgaa gtctaagaaa atattgcttt tagcttcagg agaagaaaaa
    16801 gctgatgcag tatataatac tgtttatgga gatataactc cagaagttcc tggttctata
    16861 cttcaacttc ataaagacac tatagttata gtagatgaag cagcagcaag taaattgaat
    16921 ccaaaagatt ataaattagt ataagaatag aatgtagttt aagtaaaaaa tagctatcat
    16981 tgagtaatca aatttattta atgatagcta tttttattta aattttttac ttctttttat
    17041 ttaaattagt tattattgtt taaattactt gcttattact ttttacgaaa actcgttatc
    17101 aagaagtatc caaataagat gttaaaaatt attatgttta gtgggattct atcaagcata
    17161 ataaaattac ctataattgg gatattacca actggaaaca taaagaagaa tacaggcatc
    17221 agtgatagta aaaaagatgg aaaacaaaga agaagtaatc ttggaatatc aatataccaa
    17281 ctaccatcct ttttgtattc tctaatcaat aattcagttc ctagtaataa accaatacca
    17341 ccagaggaaa ttaaattaat aaaaatgtat cttttaaagt cttgcatata accagtgtaa
    17401 aattgagtat agaaatcata atttaattta acggctaaaa aatacataag caatagaatg
    17461 attaaaaata aaagatatct aagagctttt ttcattttaa attctccctt cagaaatact
    17521 ttatttagtt ttattttgtt ggaatctgat tttatcattt agtttaagaa atccataata
    17581 ttagtgaaga atgaaataag tattatatat gaataaatat ttttagaatt aaggtgaatt
    17641 gtagtgctta atataactat aatattaatt gtttgaatat tcaataaaaa gtttgaaaaa
    17701 tatcaattct gatataaact gaataaatac agttgaatat taaattatta ttttatcaaa
    17761 aatatgcaaa gtactacacc ttcaaatatc acctaaagta attaaaaggc aattaaggtg
    17821 aagtagttca agtatttcta aaataagttt aataaattct ataataattt attttttgcc
    17881 ttaaaattgc cttaaaatcc aaacaatttt aaaactaatg tactataaaa agctggtata
    17941 atttaaatat agtaaaaaat aagatttatc ttatctattg ggatttaatt atttaatcaa
    18001 tatatttata ccatttaatt taaataaaaa gtctattaag gagtgagctt atgagtaagt
    18061 taatttatat agcagatgat gaagataata taagaaattt ggtaaagaca ttcttaaaaa
    18121 atgaaggtca tgatgttatg gattttaaaa cgggtgatga acttttagaa caatttaata
    18181 taaaggagtg tgacttagta atactagata taatgatgcc tggctcaagt ggttttgaag
    18241 tatgtacaaa acttcgtgag aaaagtacag tcccaataat catgcttact gcaagagata
    18301 cagatataga ttatattaca gggataacct taggtagtga tgattatttt acaaaaccat
    18361 ttagcccaat gtctttggtt atgagagtaa aatctatatt tagaaggata gagtttgaaa
    18421 agaaacaaaa ttatgataaa tatagtaact cgatagatat ggaattaaaa tttggtgatg
    18481 taataataaa taaaaagaat aaaatcgtta caagtaaaaa tgtaaatata gaccttacac
    18541 caaatgaata taacctattg acttatttgt ttgaaaatat agatagagca gtttccagag
    18601 atgaattatt aaacaaaata tggggatacg atatagaagt tgagacaagg gctgctgatg
    18661 atacagtgaa gagattacgt aaaaaaattt taaatacaaa tattttaata gaaactgtat
    18721 gggggtttgg atttaggttg aaggagaaaa gttaagatga agaatggaaa tgacaagaat
    18781 agaattggaa agattaaatc caaatttaaa ttaaagttct caaagaaaaa tataagcata
    18841 aaaactagga ttttgttgag tatactttgt ttgataatta tagtatttat gataatatta
    18901 gtatctttta atgtatttct tgatacatat ataaaaacaa ctgcgaatga agaattgaca
    18961 aagtctacag aaatagtaga acatatggat agtaatttta gacctattaa gccaccacaa
    19021 gatgatggaa agcttcctcc caaagtatta tctaatttta tgagaaatgt acaagataaa
    19081 gttaggatgg cagaaacaca atcagatgct gatgctatgg tagttgattc taagtataac
    19141 ctgatatttc caacaagaga ggatgatttc ttaaaaaatg ttgatgagat ggagctgatt
    19201 agggagtgta ttcaaaatga aaaattagat ttaaattcag atgaaaatac acggatatct
    19261 accagaaata aggattatta tatttcaact gtaaaaatta ctagtatata tggtgaacaa
    19321 gacaagtatc ttatactttt tatagacata tcaaaaactc taaatctagc acaaaagata
    19381 aatattgtat taatatctgt aatgtgtttt gctggaatat tagcaatatt tacagctgtg
    19441 attttatctg agaaaattgc taaaccgata aaagagttgt gtgagtttgc aaaaaaaatg
    19501 ggtcaaggag atttcaaaag aacctacttt gacttttcag ataaagaatt agtagagctc
    19561 tcaactgtta tgaataagag tgctgagtat ctggataagt atgataatga acaaaaaata
    19621 ttttttcaaa atgcatctca tgaattaagg acaccactta tgtctattaa aggttatgca
    19681 gaagctataa aatataatgt tatagatagt aaacatgcaa gtgatataat tcttgaagaa
    19741 agtgataggt taagtgatat ggttgaagat ttattataca tttctaaaat agataatata
    19801 actaaagatt ttgaacttgt agaatgtgat ttaagagaag tactttcaaa ttgtggagct
    19861 aggcaaaatg taagagctat aaataagggg ataaaattta tttatgaatt tgatgaagaa
    19921 atggttttat ttgaatgtga tgaaaaaaac atttccaaag catttatgaa tcttatagaa
    19981 aatgctttga gatacgctaa gagtgaaata aaaatagtgt gtaagtataa tcaaaaaaat
    20041 atagttgtga taatagaaga tgatggaatt ggaatcaaga aagaagattt acctcatgtt
    20101 tttgaaagat tctataaggg agtaggagga aatcatggaa ttggattatc aatagttaaa
    20161 tctattgtaa ataagcatgg tgggcgtata tacgtagaaa atggcaaaaa aggagctaaa
    20221 tttactatat tatttaaatt gtaaaaaaat tagagaaaat atagagatgt ttgaagttgt
    20281 tttttatata tatctgttgt atactgttga aaaaagaaca atattaagct gacatatatt
    20341 acgatagttg acatttaaag taaatgctta aatatagata cgaaaggaaa agtaaaatct
    20401 gaagtgtatt taggtttaag taagcttctt attttataaa aaatatgtat gaaagaacag
    20461 ctgattttta atattacaaa tttagtaacg gttgtatttg aagcttttgt catccatatg
    20521 tttttatcag actttttagg tcagaaagaa gggtatataa aagttgtaag atatgctaaa
    20581 ctgggtttta taatatgttt aggattctgt aatttaataa ctttaaaccc taaaatcaca
    20641 atgcctttaa ttttcatcct tattttttca acatcatttt tatataaagg aaatttaaag
    20701 acacgattgt ttactactgt tttattaagt atattcttta tactgtcaga aattgtagtt
    20761 acaagtattt ttgttttatt tgtaaaagaa ggctttgaaa taatgcttga aaataatagt
    20821 ataagagtat tggcgacaat tttatctaaa attgtctttt tgttgacttg taaaattatt
    20881 tgtctattta aaaaggatgt acatttagat atgcccataa agtattggct ccctttattt
    20941 ttaataccta tatttagttt atttctgtct gtttctatct ttgatgtaag taaatttttt
    21001 agtcttgaaa gtttaaaatt tctaagctta atatctagtg ttggtatttt atatattaat
    21061 tttattgtct tttatttatt taaatttatt atagataaaa ctaaactatc tatgaaatat
    21121 gaactacttg aaaaagaaat aattcataag gaagaattaa gacttagtaa tgagtgttat
    21181 aaaattattg ttgaacagac tgattctgtc gttttcgaat ggaatatcaa agaaaataaa
    21241 tcatttgtat cgcaggcttg gactgaaaaa tttggatata acaatgcatg taagaatatt
    21301 tttaaagaaa ttaaagataa agacttagtt cattctgaag ataaagctat atttgaaggt
    21361 tttcttgaaa gtattaaaaa aaagaatatg cacaatcaag cagtatatcg tttaaaaaaa
    21421 tctaatggtg aatatatttg gtgtagaaca tcaattacaa gcatttataa tgatgaaaat
    21481 gaacttttaa gagttgtcgg agtaattgta gatgtagata gtgatataaa aaaatatgaa
    21541 gagttaagaa ctagagcaga aagtgactca ttgaccaata tatacaataa aggtacattt
    21601 gaaaaattag tggaagaaac tatagttatg aatacaggtg ataaaaaaga tgcattattc
    21661 attattgatt tggatgattt taaagaaata aatgataatt ttggtcatcc atttggtgat
    21721 tttgtactaa aaacatttgc tgataagata caaactagtt ttggtagtaa agacttagtc
    21781 ggaagaattg gtggagatga atttgttgta tatatgcaag attatgttac agaagtaaat
    21841 ttgcataaaa aagctaaaga attaaataga gtactttcgg ataattacac tgatttgagt
    21901 tttagttttg atgcttctgt cagtataggt attgcaaggt atcctcaaga tggaacttca
    21961 ttttttgaat tgtttaaaaa tgcagataga gcactttata gcataaaagc atcaggtaaa
    22021 aattcatatt gtttatttga agaagaacta tatgtacagt gaatttgaat ttagatttta
    22081 tgtgagagta agatttatat atacttgtgg ctgtctgatt tataaaatat gttactttgt
    22141 atatttttat atgtttcagt aaggttaatt aacatgagca aaggtgtaag taaatttata
    22201 ttagtttaag tttacttaca cctttataaa aatacaaata atagattcat ttacattctt
    22261 aaatgattaa agctctttac cttctaaaca tgcataacat atcgttataa ttccagtaaa
    22321 taagactaaa atgcccatta ctaagattac aatttcatca tctattactc caaatgtttt
    22381 taaagtaact tatttcgctt tgtatcataa taatttgtct ctttttacat tttaacttct
    22441 gctgagattt gagttagata ttcattagaa tctcgcacac ttatatcata tagaagatat
    22501 tcttcataaa caaaatcccc aatacgtaaa ttattctgat taaaaaaatt taataacttt
    22561 tcataagcct tgtaaatagt ttcataactc cctttatgat aagcagttac ataaagtccc
    22621 tttggtttga ttgatacagg atatttcttg tactctttat taacttttgt aaataaatat
    22681 gaatatgatt caaagtcttt attaattata tcttcacctt taatgattga gccaatcgta
    22741 ccataatcac tcaattcata gttgtccata aagttaatac attcatataa aaaattttct
    22801 tcctcatctc ctaaaaactg ttctttatat gtaacaggtg ttttgaccaa gtactcttct
    22861 tcatgttctt ttaaagtaat agtattcgca tctatattac atgcactttt agtaaatgaa
    22921 atagtagatt ccattagagc ttgaatatta tttagttttt caatttcatt tttaatatca
    22981 atggttttct tttctaaaag ctcaattagt aaattaggtg ttcttttgtc tatataagct
    23041 ttaatttctt ttaaaggcat ttttacttcc ctaagtgaag aaattatacc aaaaatgcta
    23101 agttgatggt atgagtagta tctatatcca ttttctttct ttaattctgg tgagaaaaga
    23161 cctatatcat cataatggaa aagtgttttt ttattgattc cacaaatctt tgcaaattct
    23221 ccagttgtaa aataattttt tgaaacttga ctcataatta taattcctcc ttgactatcc
    23281 tgttacagta tagtttatca tagtattttg taaaggagtg tgattatatg aaaaaattaa
    23341 ttcatttttt aaaaccatat agagttttaa ttgttgtagt tttgatattt acgtttttac
    23401 aaacacttgg aactttgtat ataccaacac ttacagcaaa tatagtaaat aatggtgtag
    23461 taaagggaga tatagattat attgtaaaaa caggtttaat gatgatgata gtagctggta
    23521 taacagcttt atcagctgtg ttagtttgta aagtatcagc aaatctttca tctggatttt
    23581 gtagagatat tagagaagca gtatttataa aatcacaaga tttatcaata aatgatttta
    23641 ataatatagg tacagcatca atgattacta ggagtactag tgatattact ttaataggtc
    23701 agtctgtgtt tatgtttatt caattagtat taccagcacc aattataact gtttctggtc
    23761 tattcttagc atattctata gataaagcaa tgacaattat tattgttgta gtaatgtttt
    23821 tattcatgct ttcagcattt ttagttggta aaaagcttat aaagttgttt aaaatgatgc
    23881 aaataaagat ggataatatg aatagggtgt tacgtgaggt agtaactgga gtaagagtta
    23941 taagagcttt taataggagt cgttttgaaa aaaagagatt tgatagaact gcaatagatt
    24001 attctgaaac agcaatttca ataaataaaa tatttgcagt acttatgcct attgtaatgt
    24061 taataatgaa tttaggtata gtaagtatta tttggtttgg tgggatgaga gtcagtaatg
    24121 gaaatatgga aataggtcat ataatggcac ttgttgaata ttgtatctta attttattct
    24181 atttaatcat gggagtaatg gtatttatgt acatacctag agcaggtgca tgtgcagata
    24241 gagtaaatca gatattagat atagaacctg aaattgtaga tggaaatggt cataaagata
    24301 ctgtctcaga gagaggacat ttagtattta aaaatgtaac cttttcatat gcacaatcag
    24361 aagaacctgt gttaaataat ataacatttg aagcaaaatc tggagaagta actgcaatta
    24421 taggaagcac tggctcaggc aaatctacta ttgcaaatat aataccacgt ttttttgaaa
    24481 ttcaatctgg agaaatatct ataaatggtc aggatattaa gaagattcct caaaaagaac
    24541 ttagagataa gattggattt gtaccacaaa aagctttttt atttagtgga acaatagaag
    24601 aaaatataag gtatggaaaa gaagatgcaa gtattgaaga agtaaagcat gctgcaagta
    24661 tagctcaagc tgatgaattt atttatgata tggaagacaa gtatgactca tttgtagcac
    24721 aaggtggaaa taatttgtca ggtggtcaaa aacagagaat atccattgca agagcgttag
    24781 ttagaaagcc tgaagtatat gtctttgatg atagtttttc tgcattagat tttaaaacag
    24841 ataaaagact tagaaaagcg ttgaaaaatg aaatcaaaga ttcatcagct ataattatag
    24901 cacaacgtat aagtacaatc atggatgcta atcagattat agttttaaat gatggaaaaa
    24961 ttgtaggtat tggaaaacat aaagatttac ttgaaaattg tgaagtttat aagcaaattg
    25021 cggattcaca acttagtaaa gaagaacttg cataaaatga agaattaatc tgatatttgg
    25081 aggtgtatga tatgagtgaa aaaagaatag atgaacaaga tatagaagta cttagttttg
    25141 aagatggcat gtcagcagaa aaagctaagg atgcaaaaaa aactactaag agattattaa
    25201 aatatatggc aaaacaaaag tttaaaatat taattatttt tatttcagta ttgataagtt
    25261 cagcgcttac agttttagca cctatggtta tgggaaaagc tattgaccaa ctatttaatg
    25321 gaataaaaac tgcagtccaa acaggtacaa aattcagtgt gaattttagt actatgggag
    25381 ggatagtatc aatacttcta gggttgtact taattagtgc agtatttata tatattcaac
    25441 agtatattat gtcaggagtt gcacaaaatc tagttcttag catgagaaaa gatttaagtg
    25501 ataaactcaa taaacttcct cttaagtact atgattctca taaaaagggg gaaacactta
    25561 gtatagtaac aaatgattta gaaaaagtag cagatagttt acaagaagga ttaatgcaac
    25621 ttattacagc agtagttaca gtagttggtt ctattgtaat gatgataagt ataagtgtac
    25681 cacttacaat agtatcagct ataacattac ttgtttcatt gggtattact gttgtaattg
    25741 caagaaaatc acaagaaaga ttctctgaaa atcaaaaggc actaggagag ttaaatagca
    25801 atatagaaga gatatttact ggtcaaatag tggttaaagc atttagtaaa gaaaaggata
    25861 ctataagaaa ctttaaaata ttaaatcaaa atctttataa tgcaagtaga aaagcacagt
    25921 tttcgtcata tgcaatatct ccaattatta gatttataaa cctaattggc tatgttatta
    25981 tagcagttgt aggtgggata tttgcatcaa ctggagcaat gactcttggt agtatacaag
    26041 cgtttattca atatgtaaat caagcatcag agcctactac agaaatatct tatattgtaa
    26101 atatgcttca agcagcaata gcatctgcag agagagtttt tacagttatg gacgaggtgg
    26161 aagagataaa ggatgaagaa tcttcaaagg ttattagcat gcctaaaggt aaagtacaat
    26221 ttgagcatgt taaatttgga tatagtgatg attttattct tatgaaagat attaatatca
    26281 atctaaatgc aggagataag attgcaattg ttggacctac aggagctgga aagacaactc
    26341 ttgtaaattt acttatgaga ttttatgaga tacaaggtgg aagaattact attgatggtg
    26401 ttaatataaa agatttaaag cgtggagaac ttagaactat gtttggtatg gttcttcaag
    26461 atacatggct atttaatgga agtataaaag aaaatattgc atatagtaag tcagatgcca
    26521 caatggatga aattgtaaga gcagcaaaat cagcaagagt agaccatttt ataaggactt
    26581 taccacaagg gtatgacact atattgactg aggatgcttc taatctatct caaggtcaaa
    26641 agcaacttct aacaattgcc agggcaatac tttcagaccc atcagtattg attcttgatg
    26701 aagcaacatc tagtgtagat actagaacag aggtagaaat tcaaaaggca atgaataact
    26761 tgatgaaggg aagaactagt tttgttattg cacatagatt atcaactatt cgagatgctg
    26821 atttaatatt agtaatgaaa gaaggaacta ttattgaaca aggaagccat aaggaactta
    26881 ttgaaaagaa aggattctat gaagaacttt ataacagtca atttacaagt gaatatgatg
    26941 aagatgttgt atgattatca agttttaggc aactaaatga ttgataaata ttaagttaaa
    27001 tatagtaaca tgtaaggtta tatggaataa gagtatttct ctatttcata taaccttttt
    27061 tatttatcaa aataagataa aatttccttt ctaaattaga ttgaagaatg tatttatcaa
    27121 tataaaagta aaataatcat tattattagt gaagtaaata ttgtttgcca catatatgcc
    27181 ataaagatgc acttatttta cggagattta acaaagtagt gtaaagtata ctctaaatat
    27241 aaaataaatc aaatagagtt aaataaattg agaggtgata taaatagtga ataaaaaatt
    27301 gaactataga tttgaaatga agcacaaaat aactgaagca gatgtgttag ctttaaaatc
    27361 aagactctat ccaataatga agaaagatga aaatgcatct aaagatggaa aatatcttat
    27421 taggagttta tattttgata ctcctgaaga taaagcactt ttggataaat taaatggcgt
    27481 tgcaataaga gaaaagttta ggataagatt ttataataat gactgctctt atataaggtt
    27541 agagaaaaaa atcaaacatt acaacatgac atcaaagcta tcaactaatt taaccaaaaa
    27601 agaagtaata aatatactaa ataatgatat tgaatttcta aaagagtcga caaattgttt
    27661 attaagagaa ttttatttga aattaaaatg tgaaagatta gaggcaaaaa gcatagttga
    27721 ttacaataga gaagctttta tttatccagt tggaaatgta agagttacaa tcgatagtga
    27781 tattaaaact tctgtaaatt cggtagattt atttaataaa gatttgccaa ctgtttcggt
    27841 aattgatgag aatatgactg tattggaggt taaatatgat gaatttatac cagattttat
    27901 aaaggattta attcaaatta ataagactac ctctacagca gtttcaaagt atgcttcaag
    27961 tagattatat atttaagaat aaaggctatg atttatataa agatatacat aaataagatt
    28021 aaaaaacaaa taagttaaaa ataaaaaata aataagttaa aaataggaag gatgatttta
    28081 attatgacat ttaatgatat ttttaaatca agttttatag aaaacgtatc aggtttttca
    28141 tttgtggaca gtgctttagc actaggttca gcttttttag taggtttgtt tatatatatg
    28201 gtctataaaa agacttatat gggaattatg tattctcgac cattcaatgt gtcgcttgta
    28261 gcacttacaa tgcttacaac ttttgtaata ttggcagtta cttcaaatgt agttttatcc
    28321 ttaggtatgg ttggtgcttt atctatagta agatttagaa cagcaattaa agacccaatg
    28381 gatttagtat ttctattttg gtcattgggt tcaggtatag ttttaggagc aggtttgatt
    28441 cccttagctg tcatgggttc aataattatg ggattgattc taatattctt ttcaaataaa
    28501 acaatatctg aaactccata tatattgatg gtaaattgta ataatgaaga ttcagaagat
    28561 atagcaactg ataaaattaa aaaagtattt actaaatatc aaataaaatc aaaaagtgtt
    28621 acaccagaaa aaggaataga attagtgttt gaagtaagaa tgaaagatgg agaaacaagt
    28681 cttataaatg acttgagtca agtagatgga gttactaatg cagttcttgt aagttataat
    28741 ggtgactatg tagcatagag aggagtaatc ttttatgaaa gataaaaaat ttacccttct
    28801 tatctcgatt atgattatat ttttatgtgc tgtagttgga gtttatagta catctagcaa
    28861 caaaagtgtt gatttatata gtgatgtata tattgaaaaa tattttaaca gagacaaggt
    28921 tatggaagtt aatatagaga tagatgaaag tgacttgaag gatatgaatg aaaatgctat
    28981 aaaagaagaa tttaaggttg caaaagtaac tgtagatgga gatacatatg gaaacgtagg
    29041 tataagaact aaaggaaatt caagccttat atctgtagca aatagtgata gtgatagata
    29101 cagctataag attaattttg ataagtataa tactagtcaa agtatggaag ggcttactca
    29161 attaaatctt aataactgtt actctgaccc atcttatatg agagagtttt taacatatag
    29221 tatttgcgag gaaatgggat tagcgactcc agaatttgca tatgctaaag tctctataaa
    29281 tggcgaatat catggtttgt atttggcagt agaaggatta aaagagtctt atcttgaaaa
    29341 taattttggt aatgtaactg gagacttata taagtcagat gaaggaagct cgttgcaata
    29401 taaaggagat gacccagaaa gttactcaaa cttaatcgtt gaaagtgata aaaagacagc
    29461 tgattggtct aaaattacaa aactattaaa atctttggat acaggtgaag atattgaaaa
    29521 atatcttgat gtagattctg tccttaaaaa tatagcaata aatacagctt tattaaacct
    29581 tgatagctat caagggagtt ttgcccataa ctattattta tatgagcaag atggagtatt
    29641 ttctatgtta ccatgggatt ttaatatgtc atttggtgga tttagtggtt ttggtggagg
    29701 tagtcaatct atagcaattg atgaacctac gacaggtaat ttagaagaca gacctctcat
    29761 atcctcgtta ttaaaaaatg agacatacaa aacaaaatac cataaatatc tggaagagat
    29821 agtaacaaaa tacctagatt cagactattt agagaatatg acaacaaaat tgcatgacat
    29881 gatagcatca tatgtaaaag aagacccaac agcattttat acttatgaag aatttgaaaa
    29941 aaatataaca tcttcaattg aagattctag tgataataag ggatttggta ataaagggtt
    30001 tgacaacaat aactctaata acagtgattc taataataat tctaatagtg aaaataagca
    30061 ctctggaaat caaagtgatg aaaaagaagt taatgctgaa ttaacatcaa gcgtagtcaa
    30121 agctaataca gataatgaaa ataaaaataa aactacaaat gatagtgaaa gtaagaataa
    30181 tacagataaa gataaaagtg gaaatgataa taatcaaaag ctagaaggtc ctatgggtaa
    30241 aggaggtaag tcaataccag gggttttgga agttgcagaa gatatgagta aaactataaa
    30301 atctcaatta agtggagaaa cttcttcgac aaagcaaaac tctggtgatg aaagttcaag
    30361 tggaattaaa ggtagtgaaa agtttgatga ggatatgagt ggtatgccag aaccaccgga
    30421 gggaatggat ggtaaaatgc caccaggaat gggtaatatg gataagggag atatgaatgg
    30481 taaaaatggc aatatgaata tggatagaaa tcaagataat ccaagagaag ctggaggttt
    30541 tggcaataga ggaggaggct ctgtgagtaa aacaacaaca tacttcaaat taattttagg
    30601 tggagcttca atgataataa tgtcgattat gttagttggt gtatcaaggg taaagagaag
    30661 aagatttata aagtcaaaat aagattaaaa ttgaatataa ataattttaa tactgttaag
    30721 tctaaatatt tacttagcag tattttttag aaaataatgt atttggttac taatataagg
    30781 taataaattg ataaaaatta aatacagcat attaataata cataataaag taataaaaaa
    30841 actgtttaag tataataact tatatttaga cagttttttt catttataaa taatataaat
    30901 gaaattgaaa acatttgaag gatagaaatt ggttggataa atgtataaat tacttcacca
    30961 aaaaataatg acaaaaattg taactaagtt gtaataaaat ggtagttatt tagcataaaa
    31021 taatgtatca tagaagtata ttataaaatt aataaaataa gatttttgta agaaaactgc
    31081 tggcttttga tatttagagt gagtagttaa attatgttta ggaggggtag aatttgttgc
    31141 aaaaagtatt atggaaattt ttaaagctag tagtcggttt atttatatgt tcagttggta
    31201 tagtattgac gataaattgt aaccttggat tatctccctg ggatgtattt catcaaggac
    31261 tttcaaatca tataggaata actataggaa ctgcttctat aattgtaggc tctatagtag
    31321 ttattgctga tgtcgttctt ggagaaaatg taggatgggg aactgttttt aacatgctac
    31381 taattggatt ttttatggat ttacttttat atagtaattt aattccagag gctgataatt
    31441 tatttgtagg aataattatg ttgatattag gattggttct tttgtctgtt ggaatggtat
    31501 tttatatggg aagtggttta ggaagtggac ctagagatgg tttgatggtt gcaattcaaa
    31561 agaaaacagg aaaatcactt aaattaataa gaggtactat agaagttgga gcattaatag
    31621 taggattttt attaggtgga aaagttggta taggaactat aataagtgca tttggattgg
    31681 ggtatttcac acaaatggta tttggcttat ttaaattaga ttgtagtaag attaaacata
    31741 gatttatagt agatgatata aaatttatca aagtgtatat gagtggtgag aaaaaagctg
    31801 ctgttcaaaa tatagttaag taatttaaga ataaattcaa cgaaatgtta agtattttaa
    31861 attaacattt tgtttagata gataaattga atattaaaag aatattaaaa tatgaggatt
    31921 gctagaacac tataaaatat agatttctag taatttttta tttgttcaat atttttttag
    31981 tactagaagt cttattctct atttaatgaa tgaattaatt tataattaga gatactaaac
    32041 tttatagata ttaatataaa cacctttaaa ctgtgtattt tccaaagcct ttaaaatgct
    32101 tgtatttatt ctaaataaaa aaataaaaaa atgtcaaaaa aactcttgta ttatatccaa
    32161 attttatata taattaccta tgttgtttag aaattttaaa ctcaatgttt agtaaacaaa
    32221 atgtttagta aaataaaaca gaaactagga ggtgcaacat ggatataggt gaaaagataa
    32281 aaagattaag aacagaaaaa caactgaccc aggaagagtt agctaataga tgtgaattgt
    32341 caaagggatt tatttctcaa ttagaaaata acttaacatc accatccata gctaccctta
    32401 tagatatact tgaaatacta ggtacaaatt taagggaatt ttttaatgaa atagatgatg
    32461 aaagaatcag ttttacaaaa gaagatatgt ttgaaactga agatgaagat ttaaaatata
    32521 aattaaagtg gttaattcca aattcacaaa aaaatgaaat ggagccaata ataataacat
    32581 tgtatccagg aggacagtat aaagaagaaa agccacatga aggtgaagaa tttggatatg
    32641 tattagcagg ttcaatatat gtccatatag gcgaaaagaa aaataaagta aaaaaaggag
    32701 agagttttta ctttagaccg aaagcaaatc actatatatc aaacgagggc aaaactacag
    32761 caaaagtgat ttgggtaagt acgccaccat cattttagaa tagaggtgtt taataattgg
    32821 tagaaaatat aatagaatta agggatatat ctaaacatta tgaagaactt actgttttag
    32881 ataatttcaa tttagatata aagaaaaatg agtttctaac attgcttgga ccaagtggat
    32941 gtggaaaaac tacaacatta aaaataatag caggatttga gtatgctgat gatggtaaag
    33001 ttttatttga aggtaaggag ataaataatt tgccaccata tgaaagacaa gtaaacacag
    33061 tatttcaaaa atatgcttta tttccacaca tggatgttta tgaaaatgta gcttttggat
    33121 taaaaattaa aaagactcca aaagatgtaa tagatgctaa agtaaaagag gttttgaaac
    33181 ttgttgcact agaaggtttt gaaagacgac aagttgaatc attgagtgga ggacaacaac
    33241 aaagaattgc aatagcaaga gctctagtca atgaaccaaa ggtattatta ctagatgaac
    33301 cacttggagc acttgattta aagttaagac aagagatgca aatagaatta aagagaatcc
    33361 aaaaaaaatt aggaataaca tttgtttttg taactcatga ccaagaagag gctcttacta
    33421 tgtcagatac tattgttgta atgtataagg gcaaaattca acaaatggga acaccacaag
    33481 atatatacaa tgaacctaaa aattcatttg tagctaagtt tataggtgaa agtaacatat
    33541 ttgatggaat tatgatagaa gattataaag ttaatttctg taatagagat tttgagtgtg
    33601 ttgataaagg attcgaaaaa aacgaaaata tagaagtagt aataagacca gaagatataa
    33661 aaatggtaaa gccagaagaa ggaatgttaa aggggaaagt tacatctaca gtgtttaaag
    33721 gagtgcatta cgagatagaa ttgaatgaaa atggaagatt gtggatactt cataatacta
    33781 aaaatgctga ggttgggact gaactaggca tggatatata tccagaagat atccatataa
    33841 tgagaaaaga gaataactaa gatgaaaaga aaatcttttt tagcatatcc atatgtagtt
    33901 tggagtgcga tatttgtaat aattccttta atattagtag tattttttag ttttacaaaa
    33961 gaatcaggtg gaggttatgc ctttacactt gagaattata aagaagtaat tgaccctata
    34021 tatatgaaag tttttgggcg ttctattctt ttagcaggag gagctacatt aatatgcctt
    34081 atagttggtt atccagtggc atatataatt tcaaaggctc gtgtaagtag aagaggttca
    34141 ttaatattat tgtttattct tcctatgtgg atgaactttc ttttaagaac atatgcatgg
    34201 gttgctatat taggaaaaaa tggactttta aatacatttt taggttggtt tggaatacaa
    34261 ccattagcaa ttttgtatac taattttgct atacttttag gtatggtgta taacttttta
    34321 ccatttatgg ttcttccaat atatacagca ctatcaaaga tggataatga tttgataaac
    34381 gctgctcatg acttgggtgc aaataatatg acagttttta gaaaaattat atttcctttg
    34441 agtttgccag gtgtaatgtc tggaataact atggtattta tgccagcagt cactactttt
    34501 gcaatatcaa gactgttagg tggaggtaaa ataatgctgg ttggagattt gattgaacaa
    34561 cagtttacag ttgttggaga ttggaacttt ggttcagcta tatcgatatt tatgatgata
    34621 gtaatattaa tatctatgtc tataatgtca aaatttggag atgaatctga caaagaaggc
    34681 ggtgggttac tattttaaaa ttaaagaaaa taacttctaa tatatattta gcattagtat
    34741 tcttattttt gtatgcaccc atatttgctt tggtactatt ttctttcaat gattcaaaat
    34801 caatggcaag atggaatgga tttacatgga agtggtatgg tcaattactt caaaatgaga
    34861 gtataatgag tgctttatat tatactattg taatagctat attagcgtct gtaatatcta
    34921 caatagtagg aactataagt gctataggaa tacataagat gagaggtaag agtaagaaat
    34981 taatactaaa tgtaaactat cttcctattt taaatacaga gatagttaca gcagtagctc
    35041 ttatgagtct gtttgtattt gtaaaaatgg aatttggatt tacaacaatg ctattggcac
    35101 atataatgtt ttgtttgcca tacgttatac tttcagtttt gccaaaaatg aagcaattac
    35161 ctgataatat agaagatgca gctatggact tgggagcaac acctatatac gctcttagaa
    35221 aagtaatttt accacaaata aaaccaggta tagtttctgg atttttaatt gcatttacta
    35281 tgtcaataga tgactttata ataagtttct ttaatgctgg aaatggtgta agcaatcttt
    35341 ctatagaaat ctatggaatg gctagaagag gtataaaacc tgaaataaat gcattatcaa
    35401 caataatgtt tgcagtggtt ctaggtttac ttttactagc taataaaaaa gaatctatag
    35461 taagggggat taaataatga ttaagtttaa aaaagcaata tcccttatag ctgtaggtat
    35521 gatgacagtt actttattta caggatgtaa taaagcaact gattctacaa aagttttaaa
    35581 tgtatataat gttggagatt atatagatga gtctcttata gataagtttg aaaaagaaac
    35641 aggaatagat gttcaatatt caacttatga tactaatgaa atgatgtatc aaaaagtaaa
    35701 gagtggtagt acacactatg atttagtatt tccttcagac tatatggtag agaaaatgaa
    35761 aaatgaaggt cttttggaaa aattagattt taagaatata cctaatatga aatatttaga
    35821 taagagtttt ttaaatcctg tatacgacaa aactaatgaa tatagtgttg catatatgtg
    35881 gggaactctt ggaatattgt acaataagaa agaagttaaa gaccctatgg atagctggaa
    35941 tatactttgg aatccaaagt ataagggaaa tataatgatg tttgactctg ttagagatac
    36001 tatagggata actcttaaaa aattaggata tagcatgaat agtgtaaatc caaaagaaat
    36061 aaatgaagct aaaaatcttt taatgaaaca aaaggattta gtattggctt atgtaaatga
    36121 tgaaggtaag gatagattgc ttggtggaga agtggcaatg ggtatgctgt attctggaga
    36181 tgcagttaca ctaatggagc aaaatcctga ccttgattat gctataccta aagagggaac
    36241 taataaatgg gtagatgcta tgtgtgtacc tacaacagca caaaataaga aagaagctga
    36301 gctgtttata aactttttat tagacccaga gaatgcaaaa gtaaatgctg agtacattgg
    36361 gtattcaaca cctaatacag gagcactaaa attattagac ccagaaataa ctcagaatcc
    36421 tgttgcatat ccatctaaag aagtacttga taaatgtgaa acttttgaag atattggtga
    36481 aaatataaaa ttatatgaca aggcttggat agagttaaaa tcaaaatagg gtcttgttgt
    36541 tttaagtaaa tttacttaat aaatacttaa taaaactgtt cttttttaga aaatttattt
    36601 tctaattatt gaacagtttt attttttgtt taagttatat tcatatgcaa tttaaatagt
    36661 ttattaataa atgaaaactt atttgtataa tgactttaat aataattaga aaaaatgggc
    36721 aacttaatag aataattgga ggtgtaaatt tataaaaaat gaataggata aataaaaaaa
    36781 tagatttatt aatattggct ttgctcgttt ttatattttt aattgttgga atagtaatac
    36841 tatcgattag gacagaaaat aaattgaatg tgttcataat gttagcaata atttttttta
    36901 taataatgtt aacttattta tcaaattcag tagtggggtt gataacaagt tcaataatta
    36961 tatttatgta tacatcctat attttatata acaatattac tcacaatatg gatgttgaat
    37021 ttatatcata tatgtggata atagcaacac cagtatcttc aattataatg ggtaatctga
    37081 acaaaagcat aaatgaattg caaaatacaa ataaaaaact atctgaacag tataaggaat
    37141 tagtgactat tgatagtgaa actggactta gaaatttaaa gattttttat aatgatgtaa
    37201 atatggagat aagtaaatct attagacata atactgattt ttctcttatg atagtaaaat
    37261 taccatacta cggaaaccta cagactatat ttggtgaaaa caaaactaat aaaatagtaa
    37321 aatatattgg ttcaaatatt atagaatgta caagaaatga agatataatt tatagtttac
    37381 aaaaagatat gatagggata ctaatgccaa atacatcttt ggaaggttca aaagttgtta
    37441 aagacagaat aaagaaaagg ataaaagaat taaatttaga tttaaacaat aggggaaaat
    37501 atgtaaatat agatgtcaaa attgcatttt tacaatataa gagtagtttt ggagatagta
    37561 taaattttaa gaatatagtt gaagaggaac ttcaatatga tgtttaaaat atttaataaa
    37621 agtctattaa aattagtagt tgtattatca atagtactga caatatcaaa ttttagctta
    37681 tcttcatatg catcagaaat tacttccagc acagataagg tattaatcat atatgattct
    37741 aagaaagaaa cagcttataa tagagatatt ttaaatatta tgaggactct attgggaaga
    37801 tttagttcag atatagagct attaaaattg agtaactatg atggtgagat aaataaaaat
    37861 tattacagtc atatatttat tttaggaatc aatgaaaact catataataa tgataaaaac
    37921 actaaaaact tgattagttc cttaaattca tataaaggaa ctatatgttg gttaggatat
    37981 ggtatagaaa atcttttgga acataaaaaa tacaatttag actatgtagg aaaaacaaac
    38041 aatatagtta gtgtaaatta tagaggtaaa tcatataact tggatgaaca ttatgttttt
    38101 aacattgtag aatcaaaaga tacaagtaat aaagtaatag gaagtattaa tgatacctta
    38161 aataagtatc catatataat aaatgataaa aatctttttt atgtatcaaa attagattta
    38221 gatggagttt tattttatat attttgcgat tcacttaatg atatttttaa tattaagacg
    38281 tttgataaag gacgtatatt tgtaagaata gaagatgtac atgcttttag agaaccaaaa
    38341 aatcttgtag agatagcgga ttatttaagt agtaagaata taccatttac gattgcatta
    38401 ataccagcat atgtaaatcc taaaaatcat aaggtaataa ctttatcaga aagccctgaa
    38461 attgtcaaag caataaaata tatgcaagat aaaggtggaa ctgtgatatt gcatggatat
    38521 actcatcaat acaagaaaga agaagtttct ggagaagggt atgaattttg ggatggtaaa
    38581 aaagatgaac ccttaaaaga aaatatgaag atatttgtca aagatagagt tttgaatggt
    38641 cttagagtat gtatagagaa tggaatttat cctcttgcat ttgaagcgcc acattatgca
    38701 atggaatcag agggatataa agaattaaaa aaatattttt ctacttatat agggcaacat
    38761 caaaataatg ataaaaaatt tagcacaaat acatatcctt atattattag ggatacagag
    38821 gagtttaata tatttatacc cgaaaattta ggatatatag acccagaaga taagtttact
    38881 tttcaaaata taaaagaaaa tttagataaa ttatccattg taagaggttt tagtggaggg
    38941 ttcttctttc attcatattt aaacatagag tatctaaaaa acacaataga atatttagaa
    39001 aaacagaata tagaatttat gaatttaaga gattttaata attgggtaaa agttgatgaa
    39061 atacaaataa gaaataa
//
LOCUS       DAELLV010000011        37970 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.011, whole genome shotgun sequence.
ACCESSION   DAELLV010000011 DAELLV010000000
VERSION     DAELLV010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37970
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.011"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            22..1431
                     /locus_tag="KPD58_000517"
     CDS             22..1431
                     /locus_tag="KPD58_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG0264304.1"
                     /translation="MFIINELNKIVNEEVISFHMPGHKKGQVYKKLGYKDILKNLYKM
                     DTTEILGTDNLHFPEGIIKNSQKKASRVFKSDYTCYLVNGSSCGIQSAIMSVCSPKSK
                     IITNRDCHQSVINGCILGDIDIEYVPCEISEETNVLKGISISNVIDIIDKNLDAKAVL
                     LTYPTYYGMTYDLEYICNYAHSKKMVVIVDEAHGAHLGLSERLPKTALEQGADIVVQS
                     THKTLPAFTQSSMIHIQGDRVDIDKILNMLRITESSSPSYLLLSSLELAVDIYENKGK
                     ELMEELLNNIITFKNKVDSNINIYDTNDKTKIFISSKNMGLTGYELENILRNKYNIQV
                     ELSNYYGVLLICTIGNTVEDFSSLETALNNIVKEDFKFEKLENKKYPVVIPKKILTPR
                     EAFYKTKKSVKIYDSIGKVCGECIVPYPPGISVISPGEVISKEIIDYLMFCHSKGMIV
                     SGLKDVDLNFIQIIDLEDI"
     gene            1562..2251
                     /locus_tag="KPD58_000518"
     CDS             1562..2251
                     /locus_tag="KPD58_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBG0264305.1"
                     /translation="MKGKLIIIESGSDASGKATQTEILYERLKKEGYMIKKVEYPNYE
                     SPSSALVKMYLKGDFGKNASDVDPYIASTFFTADRYASFKTDWEEFYNEGGIIIADRY
                     TTSNMVHQASKMDICDREEYLNWLADYEFNLFKIPQPDCVIFLDVPIEFSKKLMENRK
                     NKITGEEKKDIHESDIEYLEKSYNNALYIADKYDWKKINCVLDNKLRSIESIHNEIYK
                     IVLDSIKPMER"
     gene            2254..3189
                     /locus_tag="KPD58_000519"
     CDS             2254..3189
                     /locus_tag="KPD58_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBG0264306.1"
                     /translation="MYFENIIGQDFAKKYLTNSIKKNKLNNAYMFEGMDGIGKKKFAD
                     ELSKLLLDYENLENSPDYVLIKPDGNSIKIAQIRNLQSDIVIRPHKDYKIYIINNAEK
                     MTVEAQNALLKTLEEPPNYAIIILVTNNKESLLETIKSRCDIIKFSPIPIEDLKRYLI
                     NTGIEEERAQLLAIFSRGSIENALNLSQSSEFSMMREDIQQYIQIMLDKNIVEILNIP
                     NNMEKYRGKIIALLDMMINYFRDIILLKENVNKNMLINVDKLVFIQNMSGKISYSQLS
                     KIIDIIEDAKSKIKSNCNFNISIQVMSLNIYEVIK"
     gene            3186..4079
                     /locus_tag="KPD58_000520"
     CDS             3186..4079
                     /locus_tag="KPD58_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HBG0264307.1"
                     /translation="MIKIVGIRFKSAGKIYYFDPVDFNIEQDMDVVVETARGLEYGKV
                     VVGPKDMDESELVSPLKPIIRIATEEDKKVYLENKEKAKETFELCQQKIKEHELTMFL
                     IDCEYTFDRNKLIFYFTAEGRIDFRELVKDLAAIFKTRIELRQIGVRDEAKSIGGLGP
                     CGRKLCCSSWLGDFQPVSIKMAKDQSLSLNPTKISGICGRLFCCLKYEHDVYSEAIEK
                     MPVVGALVQSDDGKGKVVEVNPLLEQIKVEFQDKTVKIYTREDIKILREPKKCDGCGG
                     KCKDNDGLDEATLRELKKLED"
     gene            4157..4903
                     /locus_tag="KPD58_000521"
     CDS             4157..4903
                     /locus_tag="KPD58_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HBG0264308.1"
                     /translation="MQLKDSERIDDLQLKGLKIIQDTNGFCFGIDAVLLANFTKIKKD
                     AKVVDLGTGTGIIPILIAGKSEAKKIIGVEIQEDVYEMATRSIKLNGLEERVEIINED
                     IKYIDKVLDINGYHVVTSNPPYMHIDGIKNPNDKKAISRHEVRCNLEDVIRAASRLVM
                     PRGKFFMIHRPTRLVDIITLGRKYKLEPKVIQFVHPRPQKAPNLVLVQFVKDGRPELK
                     ILDPLYVYGEDGNYTKELKAIYNNKDIGEL"
     gene            4904..5737
                     /gene="rsmI"
                     /locus_tag="KPD58_000522"
     CDS             4904..5737
                     /gene="rsmI"
                     /locus_tag="KPD58_000522"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBG0264309.1"
                     /translation="MSGKLYICPTPIGNLEDITYRTLRTLKEVDLIAAEDTRHSIKLL
                     NHFEISKPLTSYHEHNKDSKGDYLINKLIDGENIALISDAGMPGISDPGEEIIKQAIQ
                     NNIEIEVLPGATAFVTALVGSGMDTHRFVFEGFLDRDKKVRKAQLEELKEESRTIIFY
                     ESPHRLKDTLKDMLKILGNRNISINRELTKKYQEIIRENIETTIKIFEEKEVRGEFVL
                     IVEGFHGEKSKKENYDGLTDREYVLKLIESGMNKKDAIKIVCKDRKLKKDVVYKQVLD
                     L"
     gene            5760..6464
                     /locus_tag="KPD58_000523"
     CDS             5760..6464
                     /locus_tag="KPD58_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HBG0264310.1"
                     /translation="MEIGKIIIESLKREDTHKIFIELDNKHELEKIIPKISCMKSVGE
                     CKYHVVNCFEHSINALKELEIVLNDKDFFPIHLRKHVSNYLNTYIEEGINKLHVLKLG
                     TFLHDIGKPDSMTLDETGRVHFTNHEKIGAQIIDNMGIKLELSTETYRLISKYVRYHM
                     ILLSLYKKNDLSRKELINVFNLVDEDTIGVIMLGYADIVSTVKLLGKSGEVSVLKTYM
                     EYILTNYLYKSNYTHV"
     gene            complement(6652..7080)
                     /locus_tag="KPD58_000524"
     CDS             complement(6652..7080)
                     /locus_tag="KPD58_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HBG0264311.1"
                     /translation="MDVTDYRIIEILQDDGRISMKDLGKIVGLTSPAVSERVKRLEES
                     GVIEGYKAIVNPDSLGRVIKAFIHISLPSNGYTEFIESAAKDPRIVECHHITGDDCLL
                     LKVIVKDMYELENVIDTIKKIGSTKTSVILSTPIQAKSIL"
     gene            complement(7117..8547)
                     /locus_tag="KPD58_000525"
     CDS             complement(7117..8547)
                     /locus_tag="KPD58_000525"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBG0264312.1"
                     /translation="MRNLTLLTDLYQLTMLNGYFEKNIHEDIVVFDMFFRKNACDGGY
                     TIVCGIDQVVEYIDNLHFSDEDLEYLKNLNLFSDKFLKFLKEFKFTGDIYAVEEGTIM
                     FPNEPLITVKAPLYQAQLIETALLTIVNFQSLIATKASRVCFAAQGDPVFEFGLRRAQ
                     GPDAGIYGARAAVVGGCAGTANVLAGKMFDIPIIGTQAHSWVQKFDNELEAFQAYADV
                     YPDKCLLLVDTYDVLNSGVPNAIKVFKNISEKGYKPMGIRLDSGDLAYLSKEAKKQLD
                     NAGFSDISITASNDLDEYTITSLKAEGATINSWGVGTKLITSFDSPSLGGVYKLAASC
                     EKGVLEPKIKISENPEKINNPGYKKVIRIYNEDNKAEADLIMLHDEVIDESKPLEIFH
                     PTYTWKTKVFTNYKVKELLKPLYIKGRCKYNKKAVLEIKNHVQYELSTIWEQYKRLSK
                     PHIYKVDLSRNLWYLKTQMIDSKKVL"
     gene            8679..9263
                     /locus_tag="KPD58_000526"
     CDS             8679..9263
                     /locus_tag="KPD58_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein A"
                     /protein_id="HBG0264313.1"
                     /translation="MIIIGKIWFYMVSIGIIGSLISKNMGELNKVILSETSKGIEFAI
                     SLAGIMALWMGIMNIAKESGLIEKIGQKLNPIMRRLFPSIPKGHKAMSYIVMNIALNM
                     LGAGNGATAFGLKAMSELQTLNNKKDTATNDMIMFMVINISSIQIIPFTIIKLRMDMG
                     AQNPSEIIFTTLFATIISTAVAIITCKFFQRRYR"
     gene            9279..9800
                     /locus_tag="KPD58_000527"
     CDS             9279..9800
                     /locus_tag="KPD58_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HBG0264314.1"
                     /translation="MEIFSNLLIPIIILYIVVYGKYKKIDVYDSFVKGAIDGLKAAWD
                     ILPYIIGIFLAIGIFKTGKGLDMLEWLFTPIANMMSIPKELIGLISVKPLSGSGALGM
                     YSELANRVGIGSLVEKMGATIVGSSETIFYTMAIYYGSLKIKNTRHTLSCAMISHVAG
                     VIAAVFICYVIFV"
     misc_binding    9866..10112
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10196..12133
                     /gene="metG"
                     /locus_tag="KPD58_000528"
     CDS             10196..12133
                     /gene="metG"
                     /locus_tag="KPD58_000528"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBG0264315.1"
                     /translation="MSKPSFYVTTPIYYPSGGLHIGHTYSTVAADTIARFKRFCGYDV
                     KFLTGTDEHGEKIQKKAIEQGMSEIEYLDGMIKDIKALWNTMDISYDDFIRTTEKRHT
                     DIIQKIFTKLYEQGDIYKGEYEGRYCTPCESFWTESQLLEGNKCPDCGRETYLVKEES
                     YFFRLSKYEDRLKELFKDDSFCFPAARKNEMVANFLDKGLEDLSVTRTTFDWGIKVPF
                     DEKHVIYVWVDALCNYITALGYMTDNDEEFKKYWPANVQIVGKEIVRFHTIIWPALLM
                     ALGLEVPKQVFGHGWILFADDKMSKSKGNVVYPEPIIERYGIDTLKYFLLREFAFGQD
                     GSYTHRNFVTRINYDLANDLGNLISRTVAMVEKYNNGIIPTAKVSTNFDADLKEQAVS
                     TRENFEAEMDKMQFHEALESVWKLVRRTNKYIDETMPWALAKDETKKDELDTVLYNLC
                     ESIRIIATLINPIMNETANKIYEHIGIKGQDDITTWESTKTFGLIGENVKVFKGEPLF
                     PRLDVEKEIEELTKMFSGKPPVEEKPLEHKEEITIDDLDKIELRVGKIISCEKHPKAN
                     KLLVSQVKIGPETRQIVSGIAEYYKPEDLVGKEVTVVCNLKPVKLRGVESQGMILAAG
                     DDGDPYVLPFTQGAKDGCEVR"
     gene            12163..12933
                     /locus_tag="KPD58_000529"
     CDS             12163..12933
                     /locus_tag="KPD58_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBG0264316.1"
                     /translation="MLFDSHAHLNDESFDEDRDELIGSLKDKGVDLVVNPGADIETSI
                     TAIELAKKYDFIYSAVGVHPHDVSKLDDTAIETLRKLATENEKVVAIGEIGLDYYYDY
                     SPREEQKEWFKKQIELANELKLPIIIHDRDAHGDTFEIIKNTKNPEIGCVLHCYSGNV
                     ELAREYVKMGCYISIPGTVTFKNNKKTREVVREIPLERLFIETDSPYMSPEPHRGKRN
                     NPSQVSFVADKIAQEKGISYEEVCRVTKENAKKFFNIK"
     gene            13270..13701
                     /gene="phnG"
                     /locus_tag="KPD58_000530"
     CDS             13270..13701
                     /gene="phnG"
                     /locus_tag="KPD58_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HBG0264317.1"
                     /translation="MYTVDSTKILINMNLEKLEKIYSMIKDTSSISIIKEPNLATVMV
                     RANESVKNTTFNLGEILVTECSVKVDESLGYGIVSENNNKKAIYLAVIDAVLHSNNSK
                     FDELKNYINKSVYEENLRYEQEIIDEFSLINKTKVQFNAMD"
     gene            13716..14291
                     /gene="phnH"
                     /locus_tag="KPD58_000531"
     CDS             13716..14291
                     /gene="phnH"
                     /locus_tag="KPD58_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HBG0264318.1"
                     /translation="MVNYTQEIYRKMLDSASFPGKLNNIDNINIENNTKLLNGAIGIA
                     ITLLDQEVTFYIENYTQKDIKNIRALTMSNQIDYRKSDYIFLDINSEVDILQIKIGSF
                     EYPDESATIVHQVNDLSTYSQTKYIKLDLSGPGIKSKNSIYINGIREEFIERLSSINK
                     DYPIGIDLILVDKKGEIAFIPRSSKLSWEVL"
     gene            14294..15379
                     /locus_tag="KPD58_000532"
     CDS             14294..15379
                     /locus_tag="KPD58_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HBG0264319.1"
                     /translation="MAYVAVKGGEEAILQAKNILEFYRVKGDSLPIDVKQIEEQMSYA
                     VDKVMSEGSLYNKELSALAIKQAQGDILEASFYVRALRSTMPRITYSEPINTEEMRIK
                     RRISATFKDIPGGQYLGATRDYENRILNVELLEEDEKKNRHFRDSFINELETVDEIPE
                     FTKISNIFRKENLLQEEVCENKLEEVIDDITRNSLRFPVSRSAKLQALTRGESGVVSG
                     FAYAIVRGFGDEHPYISELRTGNVFIKVKHPIYEDEEIIIGDMLVTECEIVSKASQNK
                     TENSKTNSEDKNKMSLGYGLCMGNNENKAISMAILDKSLEPNSEKLAQDEEFVLLHID
                     GIDSLGFISHFKLPHYVDFKADVERIK"
     gene            15399..16268
                     /locus_tag="KPD58_000533"
     CDS             15399..16268
                     /locus_tag="KPD58_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HBG0264320.1"
                     /translation="MIYNYGFLDENTKKEIRRKILKAISIPGYQVPFGSRELPIAKGW
                     GTGGLQLTLSLIGKNDVVKVIDQGSDDSTNACNIRSFIASVSNVETTKDTLEATLIQT
                     RHRIPEEKLKSNQILIFQVPIPETLRIVEPSEVETRRMHSEEDYSRMWVYLYEDIVRF
                     NDISIAVEYPCKVNDRYLMNPSPIPRFDIKKLNMSDNLFLFGAGREKRIYAIPPYTKV
                     EPLEFEDYKFEEEKFEGKYCSLCNSTNTFLDEVYDSDTNEKYYSCSDTSYCEKVRLKN
                     NSIDVTIGGTWNE"
     gene            16261..17127
                     /locus_tag="KPD58_000534"
     CDS             16261..17127
                     /locus_tag="KPD58_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0264321.1"
                     /translation="MSKINDSKRLENIDKQVLIGRNINLIYGDGCEKCFESTGYESNN
                     ICKYCGSIVACNNVNLELYEGEVLGIVGESGSGKSTLLKILFFQEHANSGEVYISHYN
                     KDTNILELSDQKKRYIKNHFMGIVYQNPHLGLNLDFSSGGNIAEKLLMANLYNVEKIR
                     DRGKELLIKTNIPVERIDHKPKYFSGGMQQRVQIAKALSNNPPILLLDEVTTGLDVSV
                     QAEVLDLIREIQRELKISMIVVSHDFDVIKMLADRTIVMKNGSVIESGLTDQIMEDPQ
                     HPYTQQLINSLL"
     gene            17168..17881
                     /locus_tag="KPD58_000535"
     CDS             17168..17881
                     /locus_tag="KPD58_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0264322.1"
                     /translation="MKETILEVNNFSKDFELHALNKTITACSNISFEVSKGEFLGIIG
                     KSGAGKSTILKSIYKTYIPTTGEIIFNSEIYGNVDLAKIGDREMINLRKKEIGYVSQF
                     LKTLPRITAIELVVHSLIESGFEKDCSYDMAKDILNQFEIKENLWDAYPNNFSGGEKL
                     RLNLAQAMVKKPRLLLLDEPTASLDNQSKIYVKEKMLELKSQGTTMIGIFHDIEFMET
                     VIDKTFTMTKGTISESGVA"
     gene            17882..19051
                     /locus_tag="KPD58_000536"
     CDS             17882..19051
                     /locus_tag="KPD58_000536"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HBG0264323.1"
                     /translation="MIKLIHNANIVLEDMILENGHLIIEGDTIKKVSNDKIDYAFDFN
                     EIDEIIDAKNLYVIPGIIDIHSDAIEKEIEPRPSTLLPFNMAFYELDKKLPANGITTV
                     YHSISLGDGVGVRSIDNSLKMIKNIDSYKNIDSKSINHKVHLRYEVLYYEGLEKVLEL
                     LDENKIDYLSIMDHSPGQGQYTNPTFYKEYATKVWGVTENYVDTWLDDLVNLHDNLDW
                     NKIANIIGIAKTKNINVASHDDDTLEKMDFIKNIGINVSEFPITLEVAKHSKKLGVLT
                     CLGAPNIVRGKSHNNNLKAMDAILEDCCDIVCSDYLPSAMIKSMCIVAERINNLNKAV
                     SLFTSNPAKAVGIYDERGSIKENKKADLVLVDIDSEYPKVVNTIVNGKTVYKREV"
     gene            19055..19891
                     /locus_tag="KPD58_000537"
     CDS             19055..19891
                     /locus_tag="KPD58_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBG0264324.1"
                     /translation="MLTELHCHTSISDNNFTTEEIIKKAAEKNINILAITNHDTLSGL
                     DEALVLGEHYGITIIPGIEISAYDYKNRKRVHILGYNIDLNSQEIKNLCNPMVCDRHK
                     ASVKMVNKIIDLGYKISLEDVKKYSSKTGIFKQHIMKALIDKGYTDNIYSNLYKELFH
                     RGNGKVYVSLKYVDYRDAIKAIKNSGGICVLAHPGQMDNFSAIEEMVKVGLDGIEVYH
                     PSHNKELEKESLSYAKKYNLVVTGGSDYHGFYSQHNYELGSKSLNKEDLFKFRLALGG
                     VL"
     gene            19892..20887
                     /locus_tag="KPD58_000538"
     CDS             19892..20887
                     /locus_tag="KPD58_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0264325.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHGGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSNIDKKVKEVLEVVELSGYEKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIRSLHRKLGITTIMVTHDQEEALCLADKMVVMNRGEIIQVGTP
                     KEVYKNPETPFVADFIGTINFIDDGINKIAIRPEDIKVESNRDSKDKDIKVGEILDIE
                     FRGFNYRITVEYRSKQMKLDVVSKVAEQMKLCIGSKINFKIPKEGIVQYKSEGCA"
     gene            20905..22593
                     /locus_tag="KPD58_000539"
     CDS             20905..22593
                     /locus_tag="KPD58_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HBG0264326.1"
                     /translation="MITSKNKRRISDSTIQKYFLIFSVIVLIVFILFPLVSLLKNAFM
                     DTSGNFIGISNFSKYVENPSLIASFKNSIFVSTVSSLISLCLAFIYSYAITRTNIKFK
                     EFFKTMGMFPLFAPTMLYGISLIYLFGNKGIFTTMGLEVPLYGPLGIIISQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFCKITIPGIKYSLLSSFFVSFILAFTDF
                     GAAKVVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTLDKMASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIVCSFITLLIFILIFVSVIGSVIKLWPYNLSFTMEHY
                     TFKGILGSGIKTYLSSLKISTLTAIFGTIFVFFSAYMIEKVDKFPKLRQTAYLLSMIP
                     MALPGLVLGISYITFFNSASNPLNFLYGSTAILVIVNVVHFYSVCFITSNSSLKMLDK
                     EYELVAKSINIPFYKVFFNITVPMSITSILEIVVYYFVNSMVTVSALIFLYTPQTQTA
                     SISILKLDEIGYIGPSAAMAVLVLLTNIIVRLLYEFVTKKLKNNTQKWQQKDVA"
     gene            22605..23672
                     /locus_tag="KPD58_000540"
     CDS             22605..23672
                     /locus_tag="KPD58_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG0264327.1"
                     /translation="MKFKKLVSLGVLSTLLLSVITGCSTDSSGSDSKSDSKTKGDLTV
                     YTAIEEDSIEPYLATFKEKYPDIKLNIVRASTGDITARLLAEKDNPQADVVWGVAATS
                     LLVADDQGMLEPYAPKGSEEIESAFKDDKEVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPSSSGTGYLTVSALIQIMGEEKAWQYMDKLHENIGVYT
                     QSGSAPATSAASGEYPIGISFGYRGIKLKEEGYPVEVVFPKEGSGWDIEANALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNYAVTTISTGNPIPEGFPKKPLEQMIDNDLKSAAK
                     NREDILNKWISKYDGKTEKES"
     gene            24156..24272
                     /locus_tag="KPD58_000541"
     CDS             24156..24272
                     /locus_tag="KPD58_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein"
                     /protein_id="HBG0264328.1"
                     /translation="MDDDINMVAIRPEDIRVEFNRDFKEIIDLKLRPYRYKN"
     gene            24436..25503
                     /locus_tag="KPD58_000542"
     CDS             24436..25503
                     /locus_tag="KPD58_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG0264329.1"
                     /translation="MKFKKLVSLGILSTLLLSVITGCSNVSSGSNLKSNNKTKGELTV
                     YTAIEEDSIDTYLATFKEKYPDIKLNVLRLSVGGITARLLAEKDNPKADVLWGVAATS
                     LIILDDKEMLEPYAPKGFDKVNSAFKDDKKVPSWVGIDAWMTGITVNTKELKAKNISA
                     PSSYEDLIKPEYKDLISMPSPASSGTGYLTVSELIQIMGEEKAWQYMDKLHKNIGVYT
                     QSGSAPAILAASGEYPIGISFGNRGIKLKGEGYPVEVVFPKEGSGWEIESNALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNFAVTTIDTGNPVPEGFPKNPLEQMIDNDLKSAAR
                     NREAILNKWIEKYDGKTEKES"
     gene            25695..26225
                     /gene="rnmV"
                     /locus_tag="KPD58_000543"
     CDS             25695..26225
                     /gene="rnmV"
                     /locus_tag="KPD58_000543"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HBG0264330.1"
                     /translation="MIKEIIVVEGRDDVTAVKRAIDAELITTGGFGFPKGVMERIKAA
                     NERRGVIIFTDPDFAGEKIRKKIASEVPGCKHAFLPREEAKKDGDIGIENATPQSIIA
                     ALNKVRTENTEKRDEFKQVDLIRNGLIGNEEASLRRDALGKILGIGYGNAKQFLNRLN
                     NYGVEREEFIDALKQI"
     gene            26396..27265
                     /gene="rsmA"
                     /locus_tag="KPD58_000544"
     CDS             26396..27265
                     /gene="rsmA"
                     /locus_tag="KPD58_000544"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBG0264331.1"
                     /translation="MDRLSSHNATKEVVQKYNFKFSKSLGQNFLIDSNIIDKILSGAR
                     ITRGDNIIEVGPGIGTLTREMGKIAEKVVAIEIDRNLIPILKDTLSDLDNTEVVNQDI
                     LKVDIQELVKDKLNGGPVKLVANLPYYITTPIVMKFLEEDIPVTDIVVMVQKEVADRM
                     NAIPGTKDYGALSIAVQYYCDTEIVAKAPRHMFIPQPNVDSTVIGLHVRDKRKYDVHN
                     EDIFFKTVKASFGQRRKTLLNSLGGLGFLNKDEIREVLKEANIDEKRRGETLSIEEFS
                     VLSNIINTKVSSK"
     gene            27331..28593
                     /locus_tag="KPD58_000545"
     CDS             27331..28593
                     /locus_tag="KPD58_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264332.1"
                     /translation="MGSKRKFKRDYIILEAKDINFRYKERVLPKAFAKVEVNDEKAII
                     ALYVENLKYVKDGYKAVAIKDDHGLIDLGNIILNEQGRGEFVLDLEDYDDEIKGIALL
                     YERNIPLIGFKGNKLENYEDILFQEVEEEEYEEYEELEEEDEEIEENEESDRDEDSEE
                     EYIIEEYDEDEYEDYWEDDEDIEEGELKARTEVEDSDDEEIEEFNNYKEIENYNRVKV
                     QEEYIDIEYDEEEEEEEVFEVNREEDRFVSSKPTKSKKVQKDQNNIEKIKRANGVSQG
                     ESGNKQTSTLLMPRQIKKGLKLFKEVKPFVTDYIDKTRWWKIEINPTTLCGYTMPYLG
                     YLNVLNYTMYSDAVLHSYKYRHYLFGVQYDEYNKRKHYIYAIPGNRNEQPDNGSTGFL
                     RYQACDNRTNSLGYWLCFIDSRQRNIVR"
     gene            28744..29859
                     /locus_tag="KPD58_000546"
     CDS             28744..29859
                     /locus_tag="KPD58_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBG0264333.1"
                     /translation="MINEQRILNEFLELVQIDSLSLKEGNVAKVLVKKLEEIGCSVVI
                     DNAGEKANGETGNIIATLKGNKEGKKILFSSHMDTVTPGIGVKPIVDEANGIIKSDGT
                     TILGSDDKAGIAAILEGLRYINENNIEHTDIQVVFSICEECGLVGAKNLDYGKIDSEY
                     AFILDSGGSPGEIIVKAPAQDVINVKILGKTAHAGLEPEAGISAIMVAARAIENMNLL
                     RIDEETTANIGIINGGTATNIVTGEVNIVAEARSLKENKLDVQTKHMVETFEKAAKDF
                     GAQIEIDVNRAYTPIDVSEDSEIIKLAKKAFSNLGIEGHTESTGGGSDTNILSKNGIE
                     AITLGIGMKNAHTLSEHIAIKDLYDSAKMVVEIIKEA"
     gene            30045..30941
                     /locus_tag="KPD58_000547"
     CDS             30045..30941
                     /locus_tag="KPD58_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBG0264334.1"
                     /translation="METRYEEANKITIQSILWNIVLTIIKVIAGVIGNSSAMIADGLH
                     SASDIISSIGVLIGNYVSSRPGDREHNYGHEKAETLVSFVLSILLIFVSITIGIEAIK
                     SLFNLDALSVPSILPLVVSVISILIKEYQYRITIKVAKKINSPALKADAWHHRSDALS
                     SVAAFIGIGGSILGFKPLDPIASVVVAIFVAKVGISILISSVNELMDVSVDEEEIKEL
                     KFIVADTEGVKNLGDIKTRKHGAMAYVDLTICVDENLTVKQGHDIATKLEKHIIKHME
                     FVKGITVHVEPCTNCQGNKCNN"
     gene            31011..31808
                     /locus_tag="KPD58_000548"
     CDS             31011..31808
                     /locus_tag="KPD58_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264335.1"
                     /translation="MSKKRKLKKKAAILIGVVVFFIIYFISFRIGINMEKDKSEVPKK
                     VEKEEEDNRSLRTQIKEKKKVYISDKNIENIKIEEDMWDEIKLFFSDFKKVRNTTSSY
                     EAVYEGYSDDGVKFSTDLNFFRVYTVNKEEYYKVPVSSKDAFKKLLDESIYTSFDLLS
                     HYKDWKEVTITHGKEIKKVHRWKFDDLAHKMISKRVVGKVQPEKSKERSDYNFTINIK
                     GDNFDVKVDTMGKDYVKVTSKKNVSYYEVHTSLFDYLKNEIFEIGKK"
     gene            complement(31863..33215)
                     /locus_tag="KPD58_000549"
     CDS             complement(31863..33215)
                     /locus_tag="KPD58_000549"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBG0264336.1"
                     /translation="MNIHFIGIGGISMSALAEICINKGYQVSGSDSNESYLLDKLRDQ
                     GATIYIGQKKDNISDDVNMVVYTAAVHPDNEELVAAKEKNKLVMNRATFLGQIMREYK
                     NSIAVSGTHGKTSTTSMLSTIFEYADLDPTILVGGNLSMIGGNVKIGNSNHFITEACE
                     YVDSFLNFNPKISIVLNVEEDHLDYFSGIDEIKASFNKFGKLLPPEGYFIINGDDENI
                     DDILYDVKATIIKYGRDSDNDAVIKDIHFDNSGHGIFRIEYEGRDLGEFELSVYGLHN
                     IYNASSAIMAALVSGIDLETIRKNIKIYKGVGRRFETKGYYKNALVVDDYAHHPTELK
                     ATLAAAKKLKKSTLWCIFQPHTYTRTKSLLGEFSEAFYAADKVIITDIYAAREKDPGD
                     IHSKDLVEKLYQNNVDAIYIKEFEDIVKYLRENVKDNDLVITAGAGPIYKVADLLVEK
                     "
     gene            33434..34312
                     /gene="purR"
                     /locus_tag="KPD58_000550"
     CDS             33434..34312
                     /gene="purR"
                     /locus_tag="KPD58_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HBG0264337.1"
                     /translation="MKFKRTERIGAIVKILSDNPNKIYTLSYFTNQFNAAKSTISEDL
                     LVVKNVFEKLHLGKVITISGAAGGVKYIPKTSIAENAEFLMELCERICDKSRILSGGF
                     LYLIDLIYDPTIAAKIGKIFASNIEYVDADYVVTMETKGIPMALMTAKAMNLPLVIIR
                     KDIKVSEGPTLSMTYVSGNSSKVESMSLPRKALKPDSKVIIIDDFMRGGGTIKGMVDL
                     MNEFGAEVIGTGVFISTTNPSEKMVKDYISLIQLDVNGDKIVVEPNLKTFKDEYRNED
                     LDEEEDSEFEFEIDED"
     gene            34468..34746
                     /gene="spoVG"
                     /locus_tag="KPD58_000551"
     CDS             34468..34746
                     /gene="spoVG"
                     /locus_tag="KPD58_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HBG0264338.1"
                     /translation="MKITDVRVRKLTEEGKMKCIVSITFDNLFVVHDIKVIEGHNGLF
                     IAMPSRKVGEGNFRDIAHPINAEMRQVLEDAVLQAYHEALVQWEVAAE"
     gene            34959..36338
                     /gene="glmU"
                     /locus_tag="KPD58_000552"
     CDS             34959..36338
                     /gene="glmU"
                     /locus_tag="KPD58_000552"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBG0264339.1"
                     /translation="MNFKAIILAAGKGTRMKSKYPKVIHKVCGKEMVNHIIDVSKKSG
                     VKDTVVILGHEADVVKEKLAEEIIIAMQTEQLGTGHAVKMAKEYINDEDTIVVLCGDT
                     PLIKEETLKRLFEYHIENKYHATVLTTRVGNPTGYGRIIRDKKGDLLKIVEQKDANSE
                     EKMISEINSGIYCFNGKSLREALDLLNNNNSQGEYYLTDTAKIMRDKGLKVGAFAGST
                     IEELMGVNSRVELSKAEEIMRRRINESHMVNGVTIIDTNSTYIESDVMIGNDTIIYPG
                     VMLQGKTRIGSDCIIGMNSSITNSEIGDGTEIKNSTIIDSKVGENSTVGPYAYLRPKS
                     DLGNNVKIGDFVEVKNAIIEDGSKASHLSYIGDAHVGKNVNIGCGVVFVNYDGKNKFK
                     SIVKDNAFIGSNSNLVAPVVVEEKGYIATGSTITHDVPDGALAIARERQVIKEGWVEK
                     KNQKDDQSK"
     gene            36433..37383
                     /locus_tag="KPD58_000553"
     CDS             36433..37383
                     /locus_tag="KPD58_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HBG0264340.1"
                     /translation="MNTSGSEIKIIAGNSSKELAQKIADYIGVSVLDCEVGTFSDGEI
                     CVNMNETVRGCDVFVVQSTNSPVNDNLMELLILIDALKRASAGRITAVIPYYGYARQD
                     RKAKARDPITAKLVANLITAAGADRVLTMDLHAAQIQGYFDIPLDHLLGGTILANYFN
                     EKKIEDLVVVSPDLGSVTRSRKFANTLNGEVPIAIIDKRRPKANVCEVMNLIGDVKGK
                     NVILLDDMIDTAGTIVNAANALKEFGAKDVYACCTHGVLSGPAIERIANSEISELIVL
                     DTIQLPEEKRIDKIKIKTVAPLFGDAIRMIFSNESVSKLF"
BASE COUNT        15248 a         4162 c         6840 g        11720 t
ORIGIN      
        1 aaaaaggagt actattaata tatgtttatt attaatgaat taaataagat tgtaaatgaa
       61 gaagtaatat cttttcatat gcctggtcat aaaaagggac aggtttataa aaagttagga
      121 tataaagata tactaaaaaa tttgtataaa atggatacaa ctgagatatt aggaacagat
      181 aatttacact ttccggaagg aattataaaa aattcacaaa aaaaagcatc aagagtattt
      241 aaaagtgatt atacttgcta tttagtaaat ggtagtagct gtggaataca atctgcaata
      301 atgtcagtat gtagtcctaa aagtaaaata ataacaaata gagactgtca tcagtctgtt
      361 ataaatggat gtattttagg agatatagat atagagtatg tgccttgtga gataagtgaa
      421 gagacaaatg tcttaaaagg gatatctata agtaatgtga ttgatataat agataaaaac
      481 ttggatgcaa aagcagtact gcttacttat cctacatatt atggaatgac atatgattta
      541 gaatacatat gtaattatgc acatagcaaa aagatggtag tgatagtaga tgaagcacat
      601 ggtgcgcatt tagggcttag cgagaggctt cctaaaacag ctcttgagca aggcgcagat
      661 atagttgtac agagtacaca taaaactctt ccagcattta cacaatcgtc aatgatacat
      721 atacagggag accgagttga tatagataaa atattgaaca tgttaagaat tacagaatct
      781 tcaagccctt catatttatt attatcttct ctggaattag cagtagatat atatgaaaat
      841 aaaggaaaag aattaatgga agagctttta aataatatta taactttcaa aaataaagtt
      901 gattcaaata ttaatatata cgacacaaat gataaaacaa aaatatttat atcatctaaa
      961 aatatgggtt taacaggtta tgaactagaa aatattttaa gaaacaaata taacatacaa
     1021 gtggagttat ctaattatta tggagttctt ttaatatgta caataggaaa tactgttgag
     1081 gatttctcta gtttagaaac agcattaaac aatattgtaa aggaagactt taaatttgaa
     1141 aagttagaaa ataaaaaata ccctgttgtt attccaaaga aaatattaac acctagagaa
     1201 gcattttata aaactaaaaa aagtgttaaa atatatgaca gcataggtaa agtttgtggt
     1261 gaatgtatag ttccataccc acctggaata agtgtaatat ctcctggtga agtaataagt
     1321 aaagaaataa tagactatct aatgttttgt cattctaaag gcatgattgt gagtggattg
     1381 aaagatgtag atttaaattt tatacaaata atagatttag aagacattta aaaattatga
     1441 tataaaagta gatagaagta gatagtttaa aattgtgaag agaaataaat agactgctta
     1501 tatagagcta tctaataaaa gtactaaaaa agtaaaaagt gtttgtatgg aggatatgaa
     1561 aatgaaaggt aagttaataa ttatagagag tggttcagat gcaagtggaa aggctactca
     1621 gacagaaata ttatatgaaa gattaaaaaa agaaggctat atgattaaaa aggtagaata
     1681 tccaaattat gaatctccat catctgcctt ggtgaaaatg tacctaaaag gagactttgg
     1741 taaaaatgca tcagatgtag acccatatat agcatcaact ttttttacag ctgataggta
     1801 tgcatcattt aaaaccgatt gggaggagtt ttacaatgaa ggtggaataa ttatcgcaga
     1861 tagatataca acctctaata tggtacatca ggcctctaaa atggatattt gtgatagaga
     1921 agagtattta aattggttag ctgattatga atttaattta tttaagatac cacaacctga
     1981 ttgtgttata tttttagatg tgcctattga atttagtaaa aagcttatgg agaatagaaa
     2041 aaataaaatt acaggggaag aaaaaaagga tattcatgaa agtgacatag agtatttaga
     2101 gaaatcttac aacaatgctt tatatatagc agataaatat gattggaaaa aaataaattg
     2161 tgttttagat aacaaattaa gaagtattga aagtatacat aatgaaattt ataaaatagt
     2221 tcttgattca ataaaaccta tggagagata aatatgtatt ttgaaaatat tatagggcaa
     2281 gattttgcaa agaaatattt aacaaattct ataaagaaaa ataaacttaa taatgcgtat
     2341 atgtttgaag gaatggatgg aataggaaaa aagaaatttg cagatgaatt atcaaaacta
     2401 ttacttgatt atgaaaatct cgaaaatagt ccagactatg tactcataaa accagatgga
     2461 aatagtataa aaatagctca aatcagaaat ttacaatcag atatagtaat aagacctcat
     2521 aaagactata aaatatatat aatcaataat gctgaaaaaa tgactgtgga agctcaaaat
     2581 gcacttttaa agacgctaga ggaacctcca aactatgcta taataatctt agtcacaaac
     2641 aacaaggaat ctttattaga aactataaaa tctagatgtg atataataaa gttctcacct
     2701 ataccaattg aagacttaaa acgttatttg ataaatacag gtatagaaga agaaagagct
     2761 caattattgg ctattttttc gagaggaagt attgagaatg ctctaaatct atctcagtct
     2821 tctgagtttt caatgatgag agaagatata caacagtata ttcagataat gttagataaa
     2881 aacatagtgg agattttaaa tataccaaat aatatggaaa aatatagagg taagattata
     2941 gctttattag atatgatgat aaactatttt cgagatatca tcctattaaa agaaaatgta
     3001 aacaagaaca tgttaataaa tgtagataag ctagttttca ttcaaaatat gagtggaaaa
     3061 attagttatt ctcaattgtc caagattatt gatataatag aagatgcaaa gagtaaaata
     3121 aaaagcaatt gcaatttcaa tataagcata caagttatgt ctttaaatat atacgaggtg
     3181 attaaatgat aaagatagta ggtataaggt ttaaaagtgc tgggaaaata tattattttg
     3241 accctgttga ttttaatata gagcaagata tggatgtagt tgtagaaact gcaagaggct
     3301 tggagtatgg gaaagtagtc gttggtccaa aagatatgga tgaaagcgaa ttagtttctc
     3361 ctttgaagcc aattataaga atagctacag aagaagataa aaaagtatat ctagaaaata
     3421 aggaaaaggc aaaagaaact tttgagttgt gccaacaaaa aataaaagaa catgagttaa
     3481 caatgttttt aatagattgt gagtatacat ttgataggaa taagttgatt ttttacttta
     3541 cagcagaagg aagaatagat tttagagaat tggtaaaaga cttagctgct atatttaaaa
     3601 ctagaataga acttagacaa ataggtgtca gagatgaagc taaatctata ggaggtctag
     3661 ggccttgtgg tagaaaactt tgttgttcat cttggttagg tgattttcaa ccagtttcaa
     3721 taaaaatggc aaaagaccaa agtctatctc taaatccaac aaaaatatct ggaatatgtg
     3781 ggagattatt ctgttgctta aaatatgagc atgatgtata tagtgaagca atagaaaaaa
     3841 tgcctgttgt aggtgcattg gtgcaatcag atgatggaaa aggaaaagtt gtagaagtga
     3901 atcctttatt agaacaaatt aaagtggaat ttcaagacaa gacagtaaag atatacacaa
     3961 gagaagatat aaaaatactt agagagccta aaaaatgtga tggctgtggt ggtaaatgta
     4021 aggataatga tggattagat gaagccacac taagagagct aaagaaatta gaagattaaa
     4081 tttaaggtag ggcttttcct acctttttta aagcaattaa tgcttaaagt tattaatatt
     4141 tgaggagaat ttatatatgc aattaaaaga ttcagaaaga atagatgatt tacaattaaa
     4201 aggtcttaaa attatacaag atactaatgg tttttgtttt ggtatagatg cagttttatt
     4261 agccaatttt actaaaataa aaaaagatgc caaagtagta gatttgggta ctggaactgg
     4321 aattatacct attttgatag ctggaaaaag tgaagctaaa aaaattattg gcgtggaaat
     4381 acaagaagat gtatatgaaa tggcaactcg ttctataaaa ttaaatggat tagaagaaag
     4441 agttgagatt ataaatgaag atataaaata catagacaaa gtcttagaca taaatggtta
     4501 ccatgtagtc acatcaaatc ctccatatat gcatattgat ggaataaaaa atccaaatga
     4561 taaaaaagca atatcaagac atgaagtaag gtgcaatttg gaagatgtaa ttagagctgc
     4621 atccagattg gttatgccta gaggaaaatt cttcatgata catagaccaa caagattggt
     4681 tgatataata actttaggga gaaaatataa attagaacca aaggttattc aatttgtgca
     4741 tccaagacct caaaaagcac cgaacttagt tttggttcaa tttgtaaaag atggaaggcc
     4801 agagcttaaa atattggacc cattgtatgt ttatggagaa gatgggaatt atacaaaaga
     4861 attaaaagca atatataata ataaagatat aggagagttg taaatgagcg ggaaattgta
     4921 catatgtcca acaccaatag gaaacctaga agatataaca tatagaactt taagaacatt
     4981 aaaagaagta gacttgattg cagcagaaga tacaagacat agtataaaac ttttaaatca
     5041 ttttgaaatc tcaaaaccac taactagtta tcatgaacat aataaggatt caaagggaga
     5101 ttatttgata aataaactaa ttgatggtga aaatatagct ctgataagtg atgcaggtat
     5161 gcctggtata tctgacccag gagaagagat aataaaacaa gctatacaaa ataacataga
     5221 gatagaagta ttgccaggtg caacagcctt tgtaacggct ttagttggct ctggtatgga
     5281 cacacataga tttgtttttg agggcttttt agatagagat aaaaaagtta ggaaagcaca
     5341 attggaagaa ttaaaggaag aaagtagaac tataattttc tatgaatcac cacatagatt
     5401 gaaggataca ctaaaagata tgcttaaaat tcttggtaat aggaatattt ctataaatag
     5461 agaacttaca aaaaaatatc aagaaataat aagagaaaat atagaaacta ccataaaaat
     5521 atttgaggaa aaagaagtaa gaggagaatt tgtattaata gtagagggtt ttcatggtga
     5581 gaagtcaaag aaagaaaatt atgatgggct tacagatagg gaatatgttt taaaactaat
     5641 tgaaagtggt atgaacaaaa aagatgcaat aaaaatagtt tgtaaagata gaaagttaaa
     5701 gaaagatgtg gtttataaac aagttttaga tttataaaac ctgaatctga ggtgatttta
     5761 tggaaatagg gaaaattata atagagagtt taaagaggga agatacacat aaaatattta
     5821 tagaattaga taataagcat gaattagaaa aaataattcc taaaataagc tgtatgaagt
     5881 ctgtaggtga atgtaaatat catgtagtaa attgttttga acattctata aacgcattaa
     5941 aagaattaga aatagtatta aatgataagg atttttttcc aatacatcta agaaaacatg
     6001 tgtcaaacta tttaaacaca tatattgaag aaggaataaa taagttacat gtattaaagc
     6061 tgggaacatt tttacatgat ataggtaagc ctgacagtat gaccttagat gaaaccggaa
     6121 gagttcattt cacaaatcat gaaaaaatag gtgctcaaat aattgacaat atgggaataa
     6181 agttagagtt atcaacagag acatatagac ttataagtaa atatgtaaga tatcatatga
     6241 tacttttgtc tctatataag aaaaatgatt tgagtaggaa agaattaatt aatgtattta
     6301 acttagtaga tgaggatact ataggtgtaa taatgcttgg gtatgcagat atagtgtcta
     6361 cagtaaaatt attgggcaag agtggagaag tcagtgtgtt aaagacatat atggaatata
     6421 ttttaacaaa ttacttgtat aagagtaatt atacacatgt ataaatttag ttaatataat
     6481 tgaattttag atgattaatt gaagtttgaa atatacggat ttcaaaaaca taaaagaagt
     6541 tgtctttgtt gtaaaattat gaaagacaac tttttttata tcaaaaattg aaattttatt
     6601 aaggatactt aatggtctat taattaataa accattagta tttaatttat attacaatat
     6661 tgattttgct tgtattggcg ttgatagtat aacagaggtc ttagtagaac caattttctt
     6721 tatagtatca atcacatttt ctagctcgta catatctttt acaattactt ttaaaagtaa
     6781 gcaatcatca cctgttatat ggtgacattc tacaatccta gggtcctttg cagctgactc
     6841 aataaattct gtatatccat tgcttggaag agaaatatga ataaatgcct ttataactct
     6901 gcctaatgaa tctgggttga caatagcttt atatccttct ataacaccag actcttctaa
     6961 tcttttgact ctttctgaaa ctgcaggaga agttaaacca actatttttc ctaagtcctt
     7021 catagaaatt ctcccatcat cctgtaaaat ttctatgatt ctgtaatctg taacatccat
     7081 aaataaacag tcccccttaa ataaaaataa ttaatgctat aaaacttttt tagaatctat
     7141 catttgtgtt tttaaatacc aaagatttct agataaatca accttataaa tgtgtggttt
     7201 tgaaagtctt ttgtattgtt cccagatagt acttaattca tattgtacat gattttttat
     7261 ttcaagaaca gcttttttat tatatttgca tctgcctttt atatacaatg gtttaagaag
     7321 ctctttaact ttgtaatttg taaatacctt cgttttccat gtataagtag gatggaagat
     7381 ttctaaaggt ttagactcat ctataacttc atcatgtaac ataatcaaat ctgcctctgc
     7441 cttattgtct tcgttatata ttcttataac tttcttgtaa ccaggattat ttattttttc
     7501 tggattctca gaaatcttta tcttaggttc aagaactcct ttttcacaag atgcagctaa
     7561 tttataaact cctccaagag aaggtgaatc gaaagatgta atgagctttg tacctactcc
     7621 ccatgaattt atagtagctc cttctgcttt aagtgatgtt atggtatatt cgtctaggtc
     7681 attagatgca gtgatgctga tatctgaaaa gccagcatta tctaattgtt ttttagcttc
     7741 tttagataaa taagctaaat ctccagagtc aagtcttata cccataggtt tgtatccttt
     7801 ttcagaaata tttttgaata cttttattgc attaggaact ccactgttta atacatcata
     7861 agtatctaca agtagtaggc atttatctgg ataaacatca gcatatgctt ggaatgcttc
     7921 aagttcatta tcaaattttt gtacccaact gtgagcttgt gtaccaatta taggtatatc
     7981 aaacattttc cctgctagta cgtttgcagt tccagcacaa ccaccaacta cagcagccct
     8041 agccccatat atccctgcat ctggaccttg tgcacgacgt aatccaaatt caaatacagg
     8101 gtctccttga gctgcaaagc aaactctaga ggctttagta gcaataagag actgaaaatt
     8161 aactattgtt aacaatgctg tttcaataag ttgagcttga tataaaggtg ccttaacagt
     8221 aattaatggc tcattaggga acatgatagt tccttcctca acagcatata tatctcctgt
     8281 gaatttaaat tcttttaaaa attttaagaa cttgtcagaa aataaattta agttttttaa
     8341 gtactctaaa tcttcatctg aaaaatgaag attatctatg tattcaacaa cctgatcaat
     8401 accacaaact atagtatacc ctccatcaca agcgtttttt ctaaaaaaca tatcaaatac
     8461 aacaatgtct tcatgaatat tcttttcaaa atagccattt aacatagtaa gctgatataa
     8521 atcagtaaga agtgttagat ttctcattat ttctctcctt aattattatt aaatattaaa
     8581 aattgtaaaa actcccacac ttatactaaa acataatata gtacaacttt caagtataaa
     8641 gtattaccta actatcataa aattatttaa agaggaggat gattataata ggaaaaatat
     8701 ggttttatat ggtatcgata ggaataatag gaagcttgat ttctaaaaat atgggtgaat
     8761 taaataaagt tatattaagt gaaacatcaa aaggtataga gtttgcaata agtctagctg
     8821 gtatcatggc actctggatg gggataatga atatcgcaaa agaatcaggt ttaattgaaa
     8881 agataggtca aaaactaaat cctataatga gaagattatt tccttctatt ccaaaaggtc
     8941 ataaagcaat gtcttatata gttatgaata ttgctttaaa tatgttgggt gctggaaatg
     9001 gtgcaacagc atttggttta aaagcaatga gtgagcttca aactttgaat aacaagaaag
     9061 atacagccac taatgatatg attatgttta tggtaatcaa tatatcatct atacaaataa
     9121 ttccatttac tataatcaaa ctaaggatgg atatgggggc tcaaaatcca agtgaaataa
     9181 tttttacgac tttatttgca accataatat ctacagcagt agcaataata acatgtaaat
     9241 tttttcaaag aagatataga taaaggggat gtagatacat ggagatattt tcaaacttac
     9301 ttataccaat aataattctg tacatagtag tttatggtaa atataaaaaa atagatgtgt
     9361 atgatagttt tgtaaaagga gcaattgatg ggttaaaagc agcatgggat atcctaccat
     9421 atataatagg tatatttttg gcaataggaa tattcaaaac tggtaaaggt cttgatatgt
     9481 tagaatggct atttacacca atagcaaata tgatgagtat accaaaagaa ttaataggtc
     9541 taataagtgt aaaaccttta tctggaagtg gtgctttagg gatgtacagt gaacttgcaa
     9601 atagagttgg aataggaagt ttagtagaaa aaatgggtgc tacgattgta ggttcttctg
     9661 agactatttt ttacacaatg gcaatatact atggaagtct gaagataaaa aatactagac
     9721 atacattatc atgtgctatg attagccatg ttgcaggtgt catagcagct gtttttattt
     9781 gctatgtaat atttgtataa tttgttgaca atatgggaaa aattttataa tatagtgatt
     9841 atatagaaac aaatagtaat acaaattaca agctatgaaa agaagagtaa ataaaggttt
     9901 tttctatcag agagctaggt taggtgaaag ctagtgtcaa aagctttatt gaagatggtc
     9961 ttggagcttt ttatctgagc aagtgtgcta gggtaaaacg gttggacacg ttatagtcta
    10021 ctaaggtatc tataaaatgt ttctacattt catgtgaaac ataactatta tagatgaatt
    10081 agggtggtac cgtgaataat ctcgccccta tgttaactca taggaggcga ttttttatta
    10141 tttaaatgaa agaatcatat gattacgaac gataaaaaat taggaggtaa ataacatgag
    10201 caaaccgagt ttttatgtaa caacaccaat atactatcca agtggaggat tacacatagg
    10261 acatacttat tcaacagtag ctgcagatac aatagctaga ttcaagcgtt tctgtggata
    10321 tgatgtaaag ttcttaacag gaacagatga gcatggtgaa aaaattcaaa agaaagccat
    10381 agaacaagga atgtcagaga tagagtatct tgatggaatg ataaaagata taaaggcctt
    10441 atggaatact atggatatat cttatgatga ttttataaga actacagaaa aaagacatac
    10501 agacataata caaaagatat ttactaaact atatgagcaa ggtgatatat ataaaggtga
    10561 atatgaaggt agatactgta ctccttgtga gagtttctgg acagaatctc aattactaga
    10621 aggaaataaa tgccctgact gtggaagaga aacatattta gtaaaagaag aatcttattt
    10681 ctttagatta tctaaatatg aagacagatt aaaagagtta tttaaagatg atagtttctg
    10741 ttttcctgct gctagaaaaa atgaaatggt ggcaaacttt ttagacaaag gattagaaga
    10801 tttaagcgta actagaacga cttttgactg gggtataaaa gttccttttg atgaaaaaca
    10861 tgtaatttat gtgtgggtag atgctttatg taactatata acagcattag gttatatgac
    10921 tgataatgat gaagaattta aaaaatattg gcctgccaat gttcaaatag taggtaaaga
    10981 aatagtgaga ttccatacaa taatatggcc agcgctttta atggctttag gtctagaagt
    11041 tccaaaacaa gtatttggac atggatggat actatttgct gatgataaaa tgagtaaatc
    11101 aaaaggtaat gtggtttatc cagaacctat aatagaaaga tatggaatag acacacttaa
    11161 gtacttctta ttaagagagt ttgcatttgg gcaagatgga agttatactc atagaaattt
    11221 tgtaacgaga ataaattatg atttagcaaa tgatttagga aacttaataa gtagaactgt
    11281 agctatggtt gaaaaataca ataatggtat aataccaaca gcaaaagttt caactaattt
    11341 tgatgctgac ttaaaagaac aagctgtttc aactagagaa aactttgaag ctgaaatgga
    11401 taaaatgcaa ttccatgaag cattagagag tgtatggaaa ttagttagaa gaactaacaa
    11461 atatatagat gaaactatgc cttgggcact agctaaagat gaaactaaga aagatgaatt
    11521 agacacagtt ttatataatt tatgtgaatc tataagaata atcgcaacac taataaatcc
    11581 tataatgaat gagactgcta ataaaatata tgaacatata ggaataaaag gtcaagatga
    11641 tataactaca tgggaaagta caaaaacatt tggtcttatt ggagaaaatg taaaagtatt
    11701 taaaggtgaa cctttattcc caagattaga tgtagaaaaa gagatagaag aattaactaa
    11761 aatgttctct gggaaacctc cagtagaaga aaaaccttta gagcataaag aagaaattac
    11821 tattgatgat ttagacaaaa tagagcttag agtagggaaa ataataagct gtgaaaaaca
    11881 tccaaaagcg aataaattat tagtatcaca agttaagatt ggaccagaaa caagacaaat
    11941 agtatcagga atagctgaat actataagcc agaggattta gtaggaaaag aggttacagt
    12001 ggtatgtaac ttaaaaccag ttaaattaag aggtgtagaa tctcaaggta tgatattagc
    12061 agcaggtgat gatggagacc catatgtttt accatttaca caaggggcta aagatggatg
    12121 tgaggttcgt tagtaggtaa ttaaatagta aggagattaa agatgttatt tgactcacat
    12181 gcacatttaa atgatgaaag ttttgatgaa gatagagatg aacttattgg ttcattaaaa
    12241 gataaaggtg tagatttggt tgtaaatcca ggagcagata tagagacgtc gataacagca
    12301 attgaacttg cgaaaaaata cgactttata tattcagcag ttggggtaca tccacatgat
    12361 gtatcaaaac tagatgatac agcaatagaa actctaagaa aacttgcaac tgagaatgaa
    12421 aaagttgtag ctattggaga gattggatta gattattatt atgattattc tccaagagaa
    12481 gaacaaaaag agtggtttaa gaagcaaatt gaattagcta atgagctaaa acttccaata
    12541 ataatacatg atagagatgc acatggagat acttttgaga taataaagaa tactaaaaat
    12601 cctgaaatag gatgtgtact tcattgttat agtgggaatg tagagttagc tagagaatat
    12661 gttaagatgg gttgttatat atcaattcca ggtacggtta cttttaagaa taataaaaaa
    12721 actagagaag tagtaagaga aatacctcta gaaagattat tcatagagac tgattcacca
    12781 tatatgtcac cagagccaca tagaggaaag agaaacaatc cttctcaagt atcttttgtt
    12841 gctgataaga tagcccagga aaaagggata tcttatgaag aagtatgtag agttacaaaa
    12901 gaaaatgcaa agaaattttt taatataaaa tagacaaatt aaatcttata atgatatata
    12961 taataagtca ttataagtat aggatgagag tgcctcaaaa tgagtttttt agtttatgag
    13021 gcactctttt tgtatgaaat caaatatatt ttcatcattt caacaatgaa aaagaagttt
    13081 atcatatttt gagatagttt gtttttattt atttaattca tattttaaaa tatattttct
    13141 taacattgtt aaaaaagaat ttacaagata ttcattagaa ttaactcaac tctaatattc
    13201 acttaactta agttgtttat tatattatta aagagaagta aacgaaaagt agattaagga
    13261 ggttgaattt tgtatacagt tgatagtact aagatattga taaatatgaa cttagagaaa
    13321 ctagaaaaga tatactcaat gataaaagat acatcaagta tttctataat taaagaacct
    13381 aacttggcta cagtaatggt aagagctaat gaaagtgtaa aaaacacaac atttaattta
    13441 ggagagatat tagttactga atgtagtgta aaggtagatg aaagcttggg ttacggaata
    13501 gtttcagaaa acaataataa gaaagccatt tatttagcag ttatagatgc agttttacat
    13561 agcaataaca gtaaatttga tgagttaaag aattacataa ataaaagtgt atatgaagag
    13621 aacttaaggt atgaacaaga gattattgat gaatttagct tgattaataa aacgaaagtt
    13681 caattcaatg ctatggatta aataggagga gaatcatggt aaattataca caagaaatat
    13741 atagaaagat gctagatagt gcttcatttc ctgggaaatt aaataatata gataatataa
    13801 atatagaaaa taatacaaaa ctattgaatg gagcaattgg tatagcaatc actttattag
    13861 accaagaagt taccttctat atagaaaatt atactcaaaa agatataaaa aatataagag
    13921 cacttacaat gtcaaatcaa atagattata gaaaatcaga ttatatattt ttagatatta
    13981 attcagaagt ggatatatta cagattaaaa taggttcttt tgaatatcct gatgaaagtg
    14041 caactatagt acatcaagta aatgatttat caacgtactc tcaaactaag tatataaagc
    14101 ttgatttgag tggtcctggt ataaaatcta aaaatagtat atatataaat ggaattagag
    14161 aagaatttat agaaagatta tctagcataa ataaagatta tccaatagga atagatttga
    14221 ttttagtaga taaaaaagga gaaattgcat ttataccaag gtcatcaaaa ttaagttggg
    14281 aggtattata gaaatggctt atgtagcagt taaaggtgga gaagaagcca tattacaggc
    14341 taaaaatata cttgagtttt atagggtaaa aggagatagc ttgcctatag atgtaaagca
    14401 gattgaggaa caaatgtctt atgctgtaga caaagtaatg tctgaaggaa gtttatacaa
    14461 caaggaacta tcagcattgg ctataaaaca agctcaaggt gatatattag aagcctcttt
    14521 ctatgtaaga gctttaaggt caacaatgcc tagaattaca tattcagaac ccataaatac
    14581 agaagaaatg agaataaaaa gaagaatatc agctactttt aaagatatac caggaggtca
    14641 gtatcttggg gcaactagag actatgaaaa tagaatattg aatgtagaat tattagaaga
    14701 agatgaaaaa aagaatagac attttagaga tagttttata aatgaattag aaacagtaga
    14761 tgaaatccct gaatttacaa aaatatctaa tatatttaga aaagaaaatt tattacaaga
    14821 agaagtttgt gaaaacaaat tggaagaagt tattgatgat attacaagaa atagtttaag
    14881 atttccagtt tctaggtcag caaagttaca ggcattaact agaggtgaaa gtggagtagt
    14941 gtcaggtttt gcttatgcta ttgtaagagg ttttggtgat gaacatccat atataagtga
    15001 acttagaact ggtaatgtat ttataaaagt aaaacatccc atctatgaag atgaagaaat
    15061 aataataggg gatatgctgg ttactgaatg tgaaatagtt tcaaaagcat cacaaaataa
    15121 gactgaaaat tctaaaacta attcggaaga taaaaataag atgtctttag gatatggtct
    15181 ttgtatgggg aataatgaga ataaggctat atctatggcc atactagaca aatctttgga
    15241 gccaaatagt gaaaaactag cacaagatga agaatttgta ttgcttcata tagatggaat
    15301 agattcatta ggatttatat ctcactttaa gcttcctcac tatgtagatt ttaaagcgga
    15361 tgtagaaaga ataaaatagt ttgaggaggt aagtttaggt gatatataac tatggttttt
    15421 tagacgaaaa cacaaaaaaa gaaataagaa gaaagatatt aaaagcaata tcaatacctg
    15481 gttatcaagt tccctttggt tcaagagagt tgcctatagc taagggatgg ggtactggag
    15541 gtttacaatt aacactatca ttaattggta aaaatgatgt agtaaaagta attgaccaag
    15601 gaagtgatga ttctacaaat gcttgtaata taaggagctt tattgcaagt gtaagtaatg
    15661 tagaaaccac aaaagataca ttagaagcta cattgattca aacgagacat agaataccag
    15721 aagaaaaact taaatctaat caaatactaa tatttcaagt accaatacca gaaaccctta
    15781 gaatagtaga acctagtgaa gtagaaacaa gaagaatgca ttcagaagaa gattatagta
    15841 ggatgtgggt atatctttat gaagatatag ttagatttaa tgatatatct atagcagttg
    15901 aatatccatg taaagtaaat gatagatatt taatgaatcc atcacctata cctagatttg
    15961 atataaaaaa attaaatatg tcagacaatt tattcttgtt tggtgcaggt agagaaaaaa
    16021 gaatttatgc tattcctcca tatacaaagg tagaaccttt agagtttgag gattataagt
    16081 ttgaagagga aaagtttgaa ggaaaatact gtagtttatg taacagtaca aatacatttt
    16141 tagatgaagt atatgacagt gacactaatg aaaagtacta tagttgctca gatacaagtt
    16201 attgtgaaaa agttagatta aaaaataaca gtatagatgt gacaatagga ggtacttgga
    16261 atgagtaaaa taaatgactc taaaagatta gaaaatatag acaagcaagt attaatagga
    16321 agaaatatca atcttattta tggagatggg tgtgaaaagt gttttgaaag cacaggatat
    16381 gaaagcaata atatatgtaa gtattgtggt tctatagttg catgtaataa tgtaaattta
    16441 gaactttatg aaggagaagt ccttggaatt gtaggtgaat ctggttcagg aaaaagcact
    16501 ttattaaaaa tactgttttt ccaagaacat gcaaatagtg gagaagtata tatatcccat
    16561 tacaataaag acacaaatat actagagctt tcagaccaaa aaaagagata cataaagaat
    16621 cattttatgg gtattgtgta tcaaaatccc caccttggtt taaatttaga ctttagtagt
    16681 ggtggtaata tagcagaaaa attattgatg gcaaatttat ataatgttga aaaaataaga
    16741 gatagaggca aagaactttt aataaaaact aatatacctg tagaaagaat agaccataaa
    16801 ccaaaatact ttagtggggg aatgcaacaa cgagttcaaa tagcaaaagc tctttcaaat
    16861 aaccctccaa tattgctatt agatgaggtt acaacaggtt tagatgtttc tgtgcaagcg
    16921 gaagtattgg atttaataag agaaatccaa agagaactta agatttctat gatagttgta
    16981 tcccatgact ttgatgttat aaagatgctt gctgatagga caattgtaat gaaaaatgga
    17041 agtgtaattg agtctggtct tacagaccaa ataatggagg acccacaaca cccatatact
    17101 cagcaactta taaattcatt gctataaata tcgtttgtaa ataagcaaaa tgggggtttt
    17161 taataaaatg aaagaaacta tacttgaagt aaataatttt agtaaagact ttgagttaca
    17221 tgcattgaac aagactataa cggcatgtag taatataagt tttgaagttt caaaaggtga
    17281 gtttttagga ataataggta aatctggagc aggtaagtct accatattaa aaagtatcta
    17341 taaaacatat ataccaacaa ctggagaaat aatttttaat tcagaaatat atggtaatgt
    17401 agacctggct aaaattggag accgagaaat gataaatctt agaaagaaag aaataggata
    17461 tgtatcacag tttttaaaga cacttccacg aataactgca attgaactgg tagtacattc
    17521 tttaattgag agtgggtttg aaaaagattg ttcgtatgat atggcaaaag atatacttaa
    17581 ccaatttgaa ataaaggaaa atttatggga tgcgtatcca aataattttt ctggtggaga
    17641 aaaattaaga ttaaatttag ctcaagctat ggttaaaaag cctagacttt tgttacttga
    17701 tgaaccaact gcatcacttg ataatcaatc taaaatatat gtaaaagaaa aaatgttgga
    17761 gcttaaaagt caaggaacta caatgatagg aatttttcat gatatagagt ttatggaaac
    17821 tgttatagat aagaccttta ctatgacaaa agggactata agtgaaagtg gggtagccta
    17881 gatgataaaa ctaattcata atgcaaatat tgtattagaa gatatgattt tagaaaatgg
    17941 tcatttaatc atagagggag atacaataaa aaaagtatca aatgataaga ttgattacgc
    18001 atttgacttt aatgagatag atgaaataat agatgcaaaa aacttatatg taatacctgg
    18061 tattatagat attcacagtg atgccattga aaaggaaata gagccgagac caagtacatt
    18121 attaccattt aatatggcat tttatgaact agataaaaaa ctacctgcaa atggaataac
    18181 tacagtttat cattctatat ctttagggga tggagtagga gttagaagta ttgataactc
    18241 tttaaagatg attaaaaata ttgattcata taaaaatata gatagtaaga gcataaatca
    18301 taaagttcat ttaagatatg aagtgttgta ttatgaaggt ttagaaaagg ttttagaact
    18361 attagatgaa aataaaatag actacttatc aataatggac cattctccag gacaaggtca
    18421 gtatacaaat ccaacatttt ataaagagta tgctactaaa gtgtggggag ttacagaaaa
    18481 ttatgtagat acatggttag atgatttagt aaatctgcat gataatttag attggaataa
    18541 aatagctaat attataggta tagccaaaac taaaaatata aatgttgcat cacatgatga
    18601 tgatacatta gaaaaaatgg atttcataaa aaatataggc ataaatgtat ctgaatttcc
    18661 tataactctt gaagttgcaa aacattcaaa gaaattgggt gttctaactt gtttaggtgc
    18721 accgaatatt gtaagaggta aatctcataa taacaattta aaagcaatgg atgctatatt
    18781 ggaagattgt tgtgacattg tgtgttcgga ttatcttcca tcagctatga taaagtctat
    18841 gtgtatagtt gcagaaagaa taaataattt aaataaagca gtcagtttat ttacctcaaa
    18901 tcctgctaaa gcagttggta tatatgatga aagaggtagt ataaaagaaa ataaaaaagc
    18961 agatttagtt ttagtagata ttgatagtga atatccaaag gttgtaaata ctattgtaaa
    19021 tggaaaaaca gtttataaaa gggaggtata aagattgcta acagagttac attgtcatac
    19081 aagtatatca gataataatt ttactactga agaaataata aaaaaagcag cagaaaagaa
    19141 tataaatatc ttagcaataa caaatcatga cacattatct ggattggatg aagctcttgt
    19201 gcttggagag cattatggaa taacaataat accaggtata gaaatttcag cctatgatta
    19261 taaaaataga aaaagagttc atattcttgg atataacata gatttaaaca gtcaagaaat
    19321 aaaaaatttg tgcaatccaa tggtatgtga cagacataaa gcatctgtta agatggtaaa
    19381 taaaattata gatttaggat acaaaatatc tctggaagat gtaaagaaat attcatcaaa
    19441 gactggaata tttaaacaac atataatgaa agctcttata gataaaggat atacagataa
    19501 tatatattca aatctatata aagaattgtt tcatagagga aacggcaagg tatatgtatc
    19561 tttaaaatat gtggattaca gagatgcaat aaaagctata aaaaattctg gtggaatttg
    19621 tgtgttagct cacccgggtc aaatggataa ttttagcgct attgaagaga tggtaaaagt
    19681 aggattagat ggaatagaag tatatcatcc atctcacaac aaagaactag aaaaagagtc
    19741 gctttcttat gctaagaagt ataatctagt ggtcacagga ggttcagatt accacggatt
    19801 ttattctcaa cataattatg aacttggaag caagagctta aataaagaag atttatttaa
    19861 gtttaggtta gctttaggag gggtattata aatgtctttt ttacaagtaa aaaatgtagg
    19921 taaaagttat ggacaagtta aggtgttaaa agacatatct attgatattg aaaaaggaga
    19981 gtttatttgt ttactaggtc caagtggttg tggaaagagt acactactta gaataattgc
    20041 agggcttgag gataaacatg gtggaaaaat aataataaat gataaggata tgactaattc
    20101 accacctgaa agtagaaatt ttggtatagt ttttcaatca tatgcattat ttccaaatat
    20161 gaatgtttac aaaaacatag cctttggact agaaaataaa aacataagta aatctaatat
    20221 tgataaaaaa gttaaagaag ttctagaagt tgtagaatta agtggatatg agaaaaaata
    20281 tccatcacaa ttatcaggtg gacaacaaca aagggttgca ttagctcgtg cgattgcttt
    20341 agagccagac tttttacttc ttgatgaacc tttatctgca ttagatgcaa aagtaagact
    20401 taaacttagg gagcagatta gaagtttaca tagaaaacta ggcattacaa ctataatggt
    20461 aacgcatgac caagaagaag ctctttgttt agctgataag atggttgtta tgaatagagg
    20521 agaaataata caagttggaa cgccaaagga ggtatataaa aatccagaaa caccttttgt
    20581 agctgatttt ataggaacaa taaattttat agatgatggt attaataaga tagctattag
    20641 accagaagat ataaaagtag agtctaatag agattctaaa gataaggata taaaagtggg
    20701 agaaatttta gatatcgaat ttagaggatt taactataga attacagttg agtatcgttc
    20761 taagcaaatg aaattagatg tagtttccaa agttgcagaa caaatgaaac tgtgcatagg
    20821 ttctaaaata aactttaaga tacctaaaga aggaattgta caatataaat cagagggatg
    20881 tgcttaatag ctaggggtga ataaatgata acatcaaaaa ataaaagaag aataagtgat
    20941 agcacaatac aaaaatattt cttgattttt tcggtaattg tactaatagt atttatatta
    21001 tttccattag tatcattatt aaaaaatgca tttatggata caagtggaaa ttttatagga
    21061 atttcaaact ttagtaaata tgttgaaaat ccaagtctta tagcatcatt taaaaattca
    21121 atttttgtat ctacagtctc atctttaata tcactatgtt tagcatttat atattcatac
    21181 gcaataacaa gaacaaacat aaagtttaaa gaatttttta aaactatggg aatgtttcca
    21241 ctttttgcac caactatgtt atatggtatt tcacttatat acctttttgg aaataaggga
    21301 atctttacaa ccatgggtct ggaagtacca ctatatggtc cattaggaat aattatttct
    21361 cagattctat ttacatttcc acaagctttt ttaatattat ctgtagcact ttcgatggct
    21421 gattacagac tttatgaagc agcagatagc ttgggtgcta gtgaatttaa gaaattttgc
    21481 aaaataacaa ttcctggtat aaagtactca ttacttagtt cattctttgt atcctttata
    21541 cttgcattta cagattttgg agcagctaag gtagttggag gaaactacaa tgtactggct
    21601 actgacatat ataaacaagt agttggacaa tttaacatac caatgggagc tactgtttct
    21661 atggtaatgt taattcctgt actaatagca tttacattag ataagatggc aagtaagaaa
    21721 caaggtatga ccataactgc aaagtctatg ccatataaga ttaaggaaaa taagtttaga
    21781 gatagagtat ttttaatagt atgtagtttt ataacattgc taatttttat tttaatattc
    21841 gtttcagtta taggttcagt tataaagtta tggccatata atttaagctt tacaatggaa
    21901 cactatacat ttaaaggtat tttgggaagt ggaataaaaa catatttaag cagtttgaaa
    21961 atatcaactt taacagcaat atttggaact atatttgtat tttttagtgc ttatatgata
    22021 gaaaaggtgg ataagtttcc taaattaaga caaacagctt accttttatc aatgatacca
    22081 atggccttac ctggcttagt tttaggtata tcatatataa ctttttttaa ctcagcaagt
    22141 aatccattaa attttttata tggaagtaca gcaatattag ttatagtaaa tgtagttcat
    22201 ttttattcag tttgttttat aacctcaaat tcatctttaa aaatgttgga taaagagtat
    22261 gaattagttg caaaatccat aaatattcca ttctataaag tattttttaa tataacagtt
    22321 ccaatgtcta taacatcaat acttgaaata gtagtatatt attttgtaaa ttcaatggtt
    22381 actgtttcag cattaatatt tttatataca cctcaaactc agactgcatc aatatcaata
    22441 ctaaagttag atgagatagg atatatagga ccatcagctg ctatggcagt attagtgctt
    22501 ttaacaaata taatagtaag acttttgtac gaatttgtaa caaagaaatt aaaaaataac
    22561 acacaaaaat ggcagcaaaa agatgttgca taggggaggt aaaaatgaaa tttaagaagc
    22621 tagtttcttt aggagtatta tcaacacttt tattaagtgt aataacagga tgtagtactg
    22681 attcatcagg ttcagattca aagtctgata gtaagacaaa aggggattta acagtttaca
    22741 cagcaattga agaagatagt atagagccat atttagctac atttaaagag aaatatccag
    22801 atattaaatt aaatattgtt agggcatcaa caggagatat aacagcaaga ttattagcag
    22861 aaaaggataa tcctcaagca gatgttgtat ggggagttgc agccacaagc ttattggttg
    22921 cagatgacca ggggatgtta gaaccatatg ctccaaaagg ttctgaagaa atagagtctg
    22981 catttaaaga tgataaagaa gttccttctt gggttgggat agatgcttgg atgacaggta
    23041 taacagtaaa cactaaagaa ttaagtgata agaacatccc agttcctagt tcttacgaag
    23101 atttaataaa gccagaatat aaaggtctta tatctatgcc taatccatct tcttctggta
    23161 cagggtactt aacagtatca gcccttatac aaattatggg ggaagaaaaa gcatggcagt
    23221 atatggataa gttacatgag aatattggtg tatacactca atctggttca gctccagcta
    23281 catcagcagc ttcaggagaa tatcctattg gtatatcttt tggatataga ggtataaagt
    23341 taaaagagga aggctatcca gtagaagtag tatttccaaa agaaggttct ggatgggata
    23401 tagaagcaaa tgctcttgtg aaaaaagata atataaaaga agcatcaaaa gtatttttag
    23461 attgggctat aagtaaagat gcaatgaacg aatactctaa aaactatgca gttactacta
    23521 tttctacagg aaatccaata ccagaaggat tccctaaaaa gccattagag caaatgatag
    23581 ataatgattt aaaatcagca gcaaaaaata gagaagatat actaaataaa tggatatcaa
    23641 aatatgatgg taagacagaa aaagagagtt aagtaaaact atacaaatgg agtaaaaaga
    23701 aaaatttttt ttaagtagat tttattatta taatataagt tgagaataat ataggtcaag
    23761 aaaagtgaat attctataaa aatgtataga gtaagtatac cattcagttt gaatggtata
    23821 ttttttattt ttagagatta atttagaaat agatatacta cagtttaaaa tagtatattt
    23881 taatttcatt attaaagtat agatgttata tataaagtaa ataagatatt aaataattat
    23941 aaactaaagt aaataaaata ttgagcgatt attaaaaaga tatatatata atttaatttg
    24001 agcagtttgt tatatattat aaaaaaagag aaaaataaaa ggtaaattgt ttctggataa
    24061 cttaaaattg gaagtaagaa tatttaacta gagaagaatt atttaagttt aggttagctc
    24121 taggagtagc tgattttata ggaacaatag attttatgga tgatgatata aacatggtag
    24181 caattagacc agaagatata agggtagaat ttaatagaga tttcaaagaa attatagacc
    24241 tcaaactcag accgtatcga tataaaaact aaagttagat gatatagtgt tgtagatagg
    24301 accatcagct gttatggcag tattagtgct tttaacaaat ataatagtaa aacttttgta
    24361 tgaattgata ataaaaaaat taaaaaccaa gacataaaaa tggcaacaaa aagaagttga
    24421 ataagggagg taaaaatgaa atttaagaag ttagtctctt tgggaatatt atcaacactt
    24481 ttattgagtg taataactgg atgtagtaat gtttcatcag gttcaaactt aaagtctaat
    24541 aataagacaa aaggagagtt aacagtttat acagcaattg aggaagatag tatagataca
    24601 tacttagcta catttaaaga gaaatatcca gatattaaat tgaatgtact tagattatca
    24661 gtaggaggta taacagcaag attattagca gaaaaagata atcctaaagc agatgtgtta
    24721 tggggtgttg cagctacaag tttgataatt ttagatgaca aagaaatgct agagccatac
    24781 gctccgaaag gctttgataa ggtaaattct gcatttaaag atgataagaa agttccttct
    24841 tgggttggaa tagatgcttg gatgactggt ataacagtaa atactaaaga attaaaagct
    24901 aaaaatatat cagctcctag ttcttatgaa gatttaataa agccagaata taaagatctt
    24961 atatctatgc ctagtccagc ttcttcagga acaggatatt taacagtatc agaactcata
    25021 caaattatgg gtgaagaaaa agcatggcag tatatggata aattgcataa aaatattggt
    25081 gtatacactc agtctggttc agctccagct atattagcag cttcaggaga atatcccatt
    25141 ggtatatctt ttggaaatag aggtataaag ttaaaaggtg aaggttatcc agtagaagta
    25201 gtatttccaa aagaaggttc tggatgggag atagaatcaa atgcacttgt gaaaaaagat
    25261 aatataaaag aagcatcaaa agtattttta gattgggcta taagtaaaga tgcaatgaat
    25321 gagtattcta agaattttgc agttactaca attgacacgg gaaatccggt gccagaagga
    25381 tttccaaaga atccattaga acagatgata gataatgatt taaaatcagc agcaagaaat
    25441 agagaggcta tactaaataa atggatagaa aaatatgatg gaaagacaga aaaagagagc
    25501 taagccttgc aataaaattt agtacaaaaa gcgcctagaa tataagtttt atatctaggc
    25561 tcttttgata tatttattta aaatgcatat ttctatataa aaataaatag tttagtacct
    25621 tgattaaatt aaataaaaac cttaaaataa agagatgtaa agaaaaacta aaaacgaata
    25681 aaaggtgaat ttatatgata aaagaaataa tagtagtaga gggtagagat gacgttacag
    25741 cagttaagag agcaatagat gcagaactta taactacagg tggatttgga tttccaaaag
    25801 gtgtaatgga gcgtataaaa gctgctaatg aaagacgtgg tgttataata tttacagacc
    25861 cagattttgc aggtgaaaaa ataagaaaaa aaatagcatc agaagttcca ggatgtaaac
    25921 atgcatttct accgagagag gaagctaaaa aggatggaga tataggtatt gagaatgcta
    25981 ctcctcaaag tataatagca gctctgaata aagttagaac tgaaaataca gaaaaaagag
    26041 atgaatttaa gcaagttgac ttaattagaa atggtcttat aggtaatgaa gaagcatcac
    26101 ttagaagaga tgcacttgga aaaatacttg gaattggata tggaaatgca aaacaatttt
    26161 taaatagatt gaataattat ggagttgaaa gagaagaatt tatagatgca ttaaaacaaa
    26221 tctaaacgta atgtttaatg tatgaaaaat atgtatataa aataaattgt ggaattacgg
    26281 ttaatttaag atgaattatt tataatatac accttatttt aaatcaatgt aaatacatat
    26341 aaataagtat aattaataaa attaaatatc aaaataagtt aaaaaggagt aaataatgga
    26401 tagactatct tcacataatg caacaaagga agtagtacaa aaatataatt ttaaattttc
    26461 aaagtcttta ggtcaaaatt tcttgataga tagtaatata attgataaaa tcttatctgg
    26521 agctagaatc acaagaggag ataatatcat agaagtagga cctggtatag gaactcttac
    26581 tcgtgagatg ggtaaaatag cagaaaaagt agtggctata gaaatcgata gaaatttaat
    26641 tcctatattg aaagatacct tgtcagattt agataataca gaggttgtaa atcaagatat
    26701 actaaaagta gacatacagg aattagtaaa agataaatta aatggtgggc cagttaagct
    26761 tgtagcaaat cttccatatt atataactac tccaatagtt atgaaatttt tagaggaaga
    26821 catacctgta acagacatag tagtcatggt tcaaaaagaa gttgcagata gaatgaatgc
    26881 tattccgggt actaaagatt atggggcatt atctatagct gttcaatact attgtgatac
    26941 agagattgtg gcaaaagctc caagacatat gtttatacca caaccaaatg tagattccac
    27001 agttataggt ctacatgtaa gagataaaag aaaatatgat gttcataatg aagacatatt
    27061 ttttaaaaca gtaaaagcat cctttggaca aagaagaaaa actcttctta attcgttggg
    27121 aggtcttggt tttcttaata aagatgaaat aagagaagta ttaaaagaag ctaatataga
    27181 cgaaaaaaga aggggtgaaa ctcttagtat agaagagttt tcagttcttt caaatataat
    27241 aaatacaaaa gtatcttcta aatagaaggt acttttttta attttgttaa catatattgt
    27301 ttagagaaac tgtgttaagg ggggattgca gtgggttcta aaagaaagtt taaaagagac
    27361 tatataatac tagaagctaa agacataaac tttagataca aagaacgagt actgccaaaa
    27421 gcttttgcaa aagttgaagt aaatgatgaa aaagcaatta ttgcattata tgtagaaaat
    27481 ctaaaatatg ttaaagatgg gtataaagca gtagcaataa aagatgacca tggattaata
    27541 gacttaggaa atataatttt aaatgaacaa ggaagaggtg aatttgtttt agatttagaa
    27601 gattatgatg atgagataaa aggtattgca ttattgtatg aaagaaatat acctttaatt
    27661 ggatttaaag gaaataagct tgaaaattat gaggacatat tatttcaaga ggtagaggaa
    27721 gaagaatatg aagaatatga agagttggaa gaagaagacg aagagataga ggagaatgag
    27781 gaaagtgata gagacgaaga tagtgaagaa gaatatatta tagaagaata tgatgaagat
    27841 gagtatgaag actattggga agatgatgaa gatattgaag aaggagagtt aaaagcaaga
    27901 acggaagtag aagattctga tgatgaggaa atagaagagt tcaataatta taaggaaata
    27961 gaaaattata atagggttaa agtacaggaa gaatatatag acatagaata tgatgaagaa
    28021 gaagaggaag aagaagtttt tgaagtaaat agagaagaag ataggtttgt ttcctcaaaa
    28081 cctactaaat caaagaaagt tcaaaaagat cagaataata ttgaaaaaat aaaaagagct
    28141 aatggagtaa gtcaaggaga gagtggaaat aaacaaacca gtacattact tatgccaaga
    28201 caaataaaaa aaggtttgaa attatttaaa gaagtaaaac cttttgtaac agattatata
    28261 gataagacaa gatggtggaa aatagagata aatcctacaa cactgtgtgg atatacaatg
    28321 ccttatttag gatatttaaa cgtattgaat tacactatgt atagtgatgc agttttacat
    28381 tcatataaat acagacacta tttatttgga gttcagtatg atgaatataa taagagaaaa
    28441 cattatattt atgctatccc aggaaataga aatgaacagc cagacaatgg aagcacagga
    28501 tttttaagat atcaagcttg tgataataga acaaatagtt taggatattg gctatgtttt
    28561 atagattcta gacaaagaaa tatagttagg taaaaataat aaaaatgaaa aaatttagac
    28621 tcaattctta ttttactgaa aaaattaatg aaatgtagta caaaacttta aaaactattg
    28681 ataatattta aatattacac tacaatatat cttgaaatac ctattatatt atggaggaaa
    28741 attatgataa acgagcaaag aattttaaat gagtttcttg aattagtaca gatagatagt
    28801 ttatctttaa aagaggggaa tgttgctaaa gtattagtta aaaaattaga agaaataggt
    28861 tgttcagtag taatagacaa tgctggtgaa aaagcaaatg gagaaactgg taatataata
    28921 gctacattaa aaggaaataa agaaggaaag aaaatattat ttagttcaca tatggataca
    28981 gtaactccag gtataggggt aaaacctata gtggatgaag caaatggaat aataaaaagt
    29041 gatggtacta caatattagg ttctgatgat aaagcaggta tagcagctat attagaaggc
    29101 ttaagatata taaatgaaaa taacatagaa catactgata tacaagttgt attctctata
    29161 tgtgaggaat gtggattagt tggagctaaa aatttagact atggaaaaat agattctgaa
    29221 tatgcattta tattagatag tggtggttct ccaggtgaaa taatagtaaa agctcctgca
    29281 caagatgtaa taaatgttaa gatattagga aagactgctc atgcaggtct tgaacctgaa
    29341 gctggaataa gtgctataat ggtagctgcc agagcaatag aaaatatgaa tttacttaga
    29401 atagacgaag aaacaactgc taacatagga attataaacg gtggaacagc tacaaatata
    29461 gtaacaggag aagttaatat agttgcagaa gccagaagtc taaaagaaaa taaattagat
    29521 gttcaaacta aacacatggt agagactttt gaaaaagctg cgaaagattt tggagctcaa
    29581 attgagatag atgttaatag agcatacaca ccaatagatg ttagtgagga ttctgaaata
    29641 ataaaacttg caaagaaggc tttttcaaac ttaggtatag aaggtcatac tgaatctaca
    29701 ggaggaggta gtgatacaaa tatattaagt aagaatggta tcgaagctat aactttagga
    29761 attggtatga aaaatgctca tacactaagt gaacatatag ctataaaaga cttatatgat
    29821 tctgctaaaa tggtagtgga gataataaag gaagcataaa tttaaataat agtgtataat
    29881 atatatatcg cctagcttaa gctaggtgat ttttcatttt atagaagatt gataatctct
    29941 attgaaagca aagagtatac atttgcatat aagagagtgt ttaatttatg ttcaaatgta
    30001 ggttcttaat aatatttaga ttgtttcata agggaggaat aaatatggaa acaaggtatg
    30061 aagaagcaaa taaaataaca atacaatcaa tactttggaa tatagtatta acaataatta
    30121 aggttatagc tggtgttatt ggtaattcta gcgctatgat tgcggatgga ttacattcag
    30181 catcagatat tataagttct ataggagtac taataggcaa ttatgtatct tctagacctg
    30241 gagatagaga gcataattat ggtcatgaga aagcagagac attagtatca tttgtgttat
    30301 caatattgct tattttcgtt tctataacaa taggtataga ggcaattaaa tctttattta
    30361 acttagatgc attaagtgtt ccatcaatat taccattagt agtatcagtt atttcaatcc
    30421 tgataaaaga gtatcaatat agaataacaa taaaagttgc taaaaagata aattcaccag
    30481 ccctaaaagc tgatgcttgg catcacaggt cagatgcttt atcttcagta gctgctttta
    30541 taggtatagg tggttctatt ttagggttta aaccattaga cccaattgcg tcagttgtag
    30601 tggctatatt tgtagctaaa gtaggaatta gtatacttat aagttctgtt aatgaattga
    30661 tggatgtttc agttgatgaa gaagagataa aagaattaaa atttattgta gcagatacag
    30721 aaggtgttaa aaatcttggt gatataaaga ctagaaaaca tggagctatg gcatatgtag
    30781 atttgactat atgtgtggat gaaaatttga cagttaagca aggtcatgat attgctacca
    30841 agcttgaaaa acatataata aaacatatgg aatttgtaaa aggtataaca gttcacgttg
    30901 aaccatgtac taattgccaa ggaaataagt gtaataatta attaaaactt aaaaaaaagt
    30961 gtaaaaagtt atataattgt tttatgatta taaactctag gggtggttaa atgagcaaga
    31021 aaagaaaact aaagaaaaaa gctgcaatat taataggagt agttgttttc tttataatat
    31081 actttatatc ctttagaata ggaataaata tggaaaagga taaaagtgaa gtgccaaaga
    31141 aggtagaaaa agaagaagaa gataataggt ctttaaggac tcaaataaaa gagaagaaga
    31201 aggtttatat atcagataaa aatatagaaa acataaaaat tgaagaagat atgtgggatg
    31261 aaataaagtt atttttctca gattttaaga aagtaagaaa tactacttct tcttacgaag
    31321 cagtttatga aggttattct gatgatggag ttaaattttc tactgacctt aactttttta
    31381 gagtatatac tgtaaataaa gaagaatatt ataaagtacc agttagttct aaggatgctt
    31441 ttaagaaact tttagatgag agtatatata cttcgtttga ccttttaagt cattataaag
    31501 attggaaaga ggttactata actcatggaa aagaaattaa aaaagttcat agatggaaat
    31561 ttgatgattt agcacataag atgatttcaa agagggttgt aggaaaagtt caacctgaga
    31621 aaagtaagga aagaagtgac tataatttta ctataaatat aaaaggtgat aattttgatg
    31681 taaaagttga tacaatggga aaagattatg taaaagttac ttctaagaaa aatgtatctt
    31741 actatgaggt tcatacaagt ttatttgatt accttaaaaa tgagatattt gaaattggaa
    31801 agaaataaga ctttaagaag gctatcaaat tagatatttg atagcctaaa ttattttata
    31861 tattattttt caaccaacaa gtctgcaacc ttataaattg gaccagcacc agcagttatt
    31921 actaaatcat tatccttaac attttcacgt aagtatttta ctatatcttc aaattctttt
    31981 atatagatgg catctacatt gttttgatat aatttctcaa ccaagtcctt agaatgtata
    32041 tctcctgggt ctttttccct agcagcatat atatctgtta tgataacttt atcagcagca
    32101 tagaatgcct ctgaaaattc acctaaaaga gattttgttc tagtataagt atgtggctgg
    32161 aagatacacc ataaagttga tttttttaat tttttggcag cagctaaagt tgcttttaac
    32221 tcagttggat gatgtgcata atcatctact actaaagcat tcttatagta ccctttagtt
    32281 tcaaatcttc ttccgacacc tttataaatt tttatatttt ttcttatagt ctctaagtca
    32341 ataccagaaa caagagctgc cattatggca gaagaagcat tgtatatgtt atgaagacca
    32401 tatacagaaa gttcaaattc tcctaaatct cttccttcat attctattct aaatattcca
    32461 tgaccactat tgtcaaagtg aatatctttt attacagcat cattgtcaga atctctgccg
    32521 tattttataa tagtagcttt aacatcatac aatatgtcat caatattttc atcatcgcca
    32581 tttattatga aatagccttc tgggggtaat aattttccaa atttgttaaa agaagccttg
    32641 atttcatcta ttccagagaa ataatcaaga tggtcttctt caacatttaa aactatagat
    32701 atctttgggt taaaattaag gaaactatct acatattcac atgcttcagt tataaagtgg
    32761 ttggaattac ctatcttaac attaccacct atcatactta agttaccacc aactagtata
    32821 gttgggtcta agtctgcata ttcaaagatt gtagataaca tagatgttgt agaagttttt
    32881 ccatgtgttc cagaaactgc tatagaattt ttatattccc tcatgatttg gcctaaaaaa
    32941 gttgctctgt tcattaccaa tttatttttt tctttagcag ccacaagttc ttcattgtct
    33001 ggatgaactg ctgctgtata aacaaccata tttacatcat ctgatatatt atcttttttc
    33061 tgaccaatat aaatagttgc cccttggtcc ctaagtttgt ctaataggta tgattcgttt
    33121 gaatccgaac ctgaaacttg ataaccttta ttgatacata tttcagctaa tgcactcatg
    33181 ctgataccgc caattcctat aaagtgtata ttcataataa aaccaccttt ctttttaatt
    33241 aagtcctaca atatgaacta tttctgtggt ataataatat aatgttcaat attttataat
    33301 taatatgcat ataatattat atgtatttta tttttgaata taataaaaca taagtaatat
    33361 tataccacaa taagaattgt tatgcattag ttagtttaac gttatataaa ggtacttgta
    33421 ggaggaaata ataatgaaat ttaaaagaac agagagaatt ggagcaatag ttaaaatact
    33481 gtctgataat ccaaataaaa tatatacttt aagttatttt acaaatcaat ttaatgcagc
    33541 aaaatcaaca ataagtgaag atttattagt tgtaaaaaat gtatttgaga agctacattt
    33601 aggtaaagtt ataactattt caggagcagc aggaggagta aaatatatac ctaaaacttc
    33661 tatagcagaa aatgcagagt tcttaatgga attatgcgag agaatatgtg acaagtctag
    33721 aatactatct ggtggttttt tatacttgat tgacctaatt tacgacccta caatagctgc
    33781 taagatagga aagatattcg catcaaatat tgagtatgta gatgcagatt atgttgtgac
    33841 aatggaaaca aaaggtatac ctatggcact tatgactgca aaagctatga atttacctct
    33901 agttataata agaaaagata taaaggtatc agaaggtcct acacttagca tgacatatgt
    33961 gagtggaaac agttctaaag ttgaaagtat gagtttgcca agaaaagctc ttaaaccaga
    34021 tagtaaagtt ataattatag atgatttcat gaggggtgga ggtactataa aaggaatggt
    34081 agaccttatg aatgaatttg gtgcagaggt tataggtact ggagtattca tatctacaac
    34141 gaatccttca gaaaaaatgg taaaagatta tatttcattg attcaattgg atgttaatgg
    34201 agataaaata gtagtagaac caaacttaaa gacttttaaa gatgaatata gaaatgaaga
    34261 tttagacgaa gaagaagatt cagaatttga atttgagata gatgaagatt aataaaaaaa
    34321 atatttagaa aaatatgtaa aaataaaaag aggatatccc tagttgttca tagaattatt
    34381 taaaaaacag tttatattaa tttattccag cgataataat tttaatataa attaatttct
    34441 gtcaaaatca aagggggata ttttggtatg aagataactg acgtaagagt aagaaaatta
    34501 acagaagaag gtaagatgaa atgtatagtt tcaataactt tcgataattt gtttgtagtg
    34561 catgatataa aagttataga aggccataat gggctattta tagcaatgcc tagcagaaaa
    34621 gtaggcgaag gaaatttcag agatatagct catccaataa atgcagaaat gagacaagtt
    34681 ttagaagatg cagttttaca agcgtatcat gaagcattag ttcaatggga agtagctgct
    34741 gaataataat aaatatataa aatggagaga gcccgaacat ttaggctctt tttaaatgta
    34801 gaatttctat taaaatatgt actttttagt aaaaaagtgc tatattatta gtgataatgt
    34861 atatgttgta tacaaattgt tagataaatg tattataatg tatattgatt aagagaataa
    34921 tacgaataaa attaaattag attgaatgga gtgtatcaat gaactttaaa gctataatcc
    34981 ttgctgctgg caaaggaaca agaatgaagt ctaaataccc aaaagttata cataaagtat
    35041 gtggaaaaga aatggttaac catattatag atgtttcaaa aaaatcaggg gtaaaagata
    35101 ctgtcgtgat tttaggacat gaagctgatg tagttaaaga aaagcttgca gaagaaatta
    35161 taatagccat gcaaacagaa caacttggga caggacatgc tgtaaagatg gctaaagaat
    35221 atattaatga tgaagataca atagttgtac tttgtggaga tactccactt ataaaagaag
    35281 agacactaaa gagactattt gaatatcata tagaaaataa atatcatgct acagttctta
    35341 caacaagagt tggaaatcca acaggttatg gtagaataat aagagataag aagggtgacc
    35401 ttcttaaaat agtggaacaa aaagatgcaa attcagaaga aaagatgata agtgagataa
    35461 actctggaat ttattgtttt aatggaaaga gtcttagaga agcactagat ttgctaaata
    35521 ataacaattc acaaggtgaa tattatttaa ctgatactgc taaaataatg agagataaag
    35581 gattaaaagt tggtgcattt gcaggttcaa ctattgaaga acttatgggc gttaattcaa
    35641 gagtagaatt atcaaaggca gaagaaatta tgagacgaag aataaatgaa tctcatatgg
    35701 taaatggagt aactataata gacactaact ctacctatat agaatctgat gttatgatag
    35761 gcaatgatac tataatatat ccaggtgtta tgttacaagg aaaaacaagg ataggttcag
    35821 attgtataat tggaatgaat tcatctataa caaattcaga aataggtgat ggtacagaaa
    35881 taaaaaattc aactataatt gatagtaagg ttggagaaaa ttctactgtt gggccatatg
    35941 catacttaag accaaagagt gacttaggaa ataatgtaaa aataggcgat tttgtagaag
    36001 taaaaaatgc tattatagaa gatggctcaa aagcatctca cctttcttat ataggagatg
    36061 cacatgtcgg taaaaatgta aatattggat gtggagtagt atttgtaaac tatgatggta
    36121 aaaataaatt taaatcaata gtaaaagaca atgcgtttat aggttcaaat tctaatttag
    36181 ttgcaccagt agttgtagaa gaaaaaggat atatagcaac aggttcaaca ataacacatg
    36241 atgtcccaga tggtgctttg gcaatagcaa gagaaagaca agttatcaaa gaaggatggg
    36301 tagaaaaaaa gaatcaaaaa gacgaccaaa gtaagtaatt atgtagttta ttattttggc
    36361 taaaaaattt agctcgaaat tattataaat tatattcatt agtattttaa aattttcagg
    36421 agggttacat aaatgaatac tagcggaagc gagattaaaa ttattgcagg taattcatcg
    36481 aaagagttag cacaaaaaat agctgactat ataggtgtat cagtactaga ttgtgaagta
    36541 ggtacattta gtgatggtga aatatgtgta aacatgaatg agacagtaag aggttgtgat
    36601 gtgtttgtag tgcaatctac taacagccca gtaaatgata acttaatgga gttattaatc
    36661 ttaattgatg cgttaaagag agcatcagca ggtagaataa cagcagttat tccttactat
    36721 ggatatgcaa gacaagacag aaaggctaag gcaagagatc caatcactgc taaattagtt
    36781 gcaaatttaa taactgctgc tggagcagat agagtattaa caatggattt acatgcagct
    36841 caaatacaag gatattttga tataccatta gaccacttac taggtggaac aatattagca
    36901 aattatttta atgaaaagaa aatagaagat ttagtagtag tatctcctga cttaggaagt
    36961 gttactagat caagaaaatt tgctaatact ttaaatggag aagttccaat agctataata
    37021 gataaaagaa gaccgaaagc taatgtatgt gaagttatga acttaatagg tgatgttaaa
    37081 ggtaaaaatg ttatactatt agacgatatg atagatacag caggaacaat agttaatgct
    37141 gcaaatgcac ttaaagagtt tggagcaaaa gatgtttatg cttgttgtac tcatggtgtg
    37201 ttatcaggtc cagctattga aagaattgct aattcagaaa taagtgagtt aatagttctt
    37261 gatactatac aacttcctga agagaaaaga atagataaga taaagataaa aacagtagct
    37321 cctttatttg gagatgctat aagaatgata ttctctaatg aatcagtaag taaattattc
    37381 taatttattg tatttatgag agttgtttaa aaggatataa accttttgag caactctttt
    37441 ttgattttat acttatggaa tgttattttt ttcttgttag aaaggtatat attattgtaa
    37501 aaagagatta tagtttaaaa ttatgtatat ttaagtaaaa taaacctaat aaagggtata
    37561 ttattcaaaa tattatgaat ataaaaaata ttacaaaaaa gtattgaaaa aattcagatt
    37621 atgtagtatt atgtaaatat aaataaagaa agaggtgttt ccaatgggaa ggttagaaga
    37681 tgaaccccta ataattaaat gattcagtca caatctaaag tgaatataaa aatctattga
    37741 aagtttattt ataagaattg aaaatagtat ctattctatc tttagtgttt gtgagagggt
    37801 tctggattat ataagattac tgtaaagagt aattttgcac aataagcata tctgtaaatg
    37861 aagagtttcg tttatataat gattgtggct gaatcataac tataatagat agaatgttta
    37921 taatagatga ttttataaaa tactatattg taatagcttt tagctttcaa
//
LOCUS       DAELLV010000012        35957 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.012, whole genome shotgun sequence.
ACCESSION   DAELLV010000012 DAELLV010000000
VERSION     DAELLV010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35957
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.012"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(359..871)
                     /locus_tag="KPD58_000554"
     CDS             complement(359..871)
                     /locus_tag="KPD58_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG0264341.1"
                     /translation="MLEVIKNRHSIRTYIDKNIEEDKITEILKSAMQAPSSKNAQPWE
                     FIIVDDKELLKQLSKSQHRAKHIEFAPLCIVVLGNRDKFLKPGKWIQDLGACTQNLLL
                     EVTNQGLAACWAGVFPKNKVVNKVRQTLDLPVKLVPYALISIGYSEEKNEFIDRFDEN
                     KIHRNVYKNR"
     gene            1321..1797
                     /gene="purE"
                     /locus_tag="KPD58_000555"
     CDS             1321..1797
                     /gene="purE"
                     /locus_tag="KPD58_000555"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBG0264342.1"
                     /translation="MKVAVVMGSKSDYPKLEEGIKLLEKYGIEVVARALSAHRTPEQL
                     SIFLKEIEDDTDVIIAAAGKAAHLPGVIASQTLIPVIGLPIKSSTMDGLDSLLSIVQM
                     PKGIPVATVTIDLGLNAALLALQIMTLKYPKLKEDLKSYREEMAQKVLEDDKNLRG"
     gene            1803..2501
                     /locus_tag="KPD58_000556"
     CDS             1803..2501
                     /locus_tag="KPD58_000556"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBG0264343.1"
                     /translation="MLLYEGKAKQVYSTDNENEYVVYYKDDATAFNGEKKAEISSKGI
                     LNNKISTIIFEMLKENNINTHFIKSLSDREMLVKKVEILPLEVIVRNIAAGSICKRVG
                     LEEGVVFDEPIFEISYKNDAYGDPMLNDDYAVAMKLATREELKFLREETLKINELLKA
                     FFLKLNLKLVDFKIEFGKDSEGNIILADEVSPDTCRLWDVNTNEKLDKDRFRKDLGDL
                     VEGYTEVLSRMNNK"
     gene            2513..3880
                     /gene="purF"
                     /locus_tag="KPD58_000557"
     CDS             2513..3880
                     /gene="purF"
                     /locus_tag="KPD58_000557"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBG0264344.1"
                     /translation="MCGVLGIYSNKDVTKELYYSLYSMQHRGQESCGLALLDDGEIKY
                     KKDMGLVGDVFKENELSKLKGNIGIGHVRYSTAGGSHVSNCQPLVGSCRKRQLAIAHN
                     GNLVNANYLKDMLEEDGYMFQTNSDTEVILYILARYYKGDIVESLKVTMDYIKGAYAL
                     VIMSQEELVAVRDPHGFRPLVLGKKGDEYIFASENCAIDILGGEVIRDVEPGEIIVVK
                     DGELKSYFYSENYKPVKKSCIFEHIYFARNDATIDNVNAYEFRIKCGERLAQNETVKA
                     DMVVPVPDSGWPGAIGYANASGLKISEGLVKNRYVGRTFIKPTQEEREIAVKIKLNPL
                     STIIKGKSIILVDDSIVRGTTSKQLVKSLREAGAKEIHLRITSPPVAYSCYYGIDTPN
                     RSKLIASSNNVEEMREYIGCDSLKFLDIEGMLDATEHKSTFCKACFDGEYPVKKIDKE
                     ELLSC"
     gene            3874..4950
                     /locus_tag="KPD58_000558"
     CDS             3874..4950
                     /locus_tag="KPD58_000558"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBG0264345.1"
                     /translation="MLTYKESGVDIDEGNRAVDLIKGKIKGTYDGNVVGDLGNFSGLY
                     SLKDFVGMKEPVLLASTDGVGTKLKIAQMMDKHDTVGIDLVAMCVNDLICQGAKPLFF
                     LDYIALGKLVPEHIEKIVGGIADGCKMSGCALIGGETAEMPGMYGEDDYDLAGFSVGI
                     ADKEKIVSGNNVKSGDVLVGISSSGVHSNGFSFIRKIFLETYNYKMEQYVEELGMTVG
                     EALLTPTKIYVKLALDVLAKHDIKAIAHITGGGLIENITRVIPKGLGLDINKKSWEKP
                     PIFKMIEGFNAVDERELHKSFNMGIGLVLIVDKENADDVVNFINNRENDNAAYVDKKY
                     SELLEDKAYIIGEVVDSHEGVELC"
     gene            4944..5537
                     /locus_tag="KPD58_000559"
     CDS             4944..5537
                     /locus_tag="KPD58_000559"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBG0264346.1"
                     /translation="MLNIGVLISGGGTNLQAVIDGTESGEIKGQVKVVISSKQGAYGL
                     ERAKNHNIKAICETDEDKIIEILKENKIDLVVLAGYLKIISPKLVNEFRNKMINIHPS
                     LIPSFCGAGFYGEKVHQGVIDYGAKVTGATVHFVDEGADTGPIIMQDVVKVNQDDDAK
                     TLAKRVLEVEHRILKESISLFCENKLKLQGRRVFINE"
     gene            5530..7062
                     /gene="purH"
                     /locus_tag="KPD58_000560"
     CDS             5530..7062
                     /gene="purH"
                     /locus_tag="KPD58_000560"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBG0264347.1"
                     /translation="MSKRALISVTDKTGVVEFAKELNKLDYEIISTGNTFKTLKENGV
                     NVMQVEDVTNFPEILDGRVKTLNPYIHGGILYKRDKESHVETVNEHKIHSIDLVAVNL
                     YDFEGTLKAGKSHDEIIENIDIGGPSMIRSAAKNYKDVIVVVDIKDYDSIIEKLKTDT
                     MTLEDRKKLSYKAFSTTGRYDALISSYFAGEVGDTYPDILNLTFQKEQTLRYGENPHQ
                     NGFLYSQSNAKNPILNYEQLGGKELSFNNLNDLHGCLEVMREFKDSEEVVSVAIKHAN
                     SCGVGLGKDAFEAYTKCYEADKVSIFGGIVGITSTIDKATAEKLNEIFLEIVVAYDFE
                     PEALEILKQKKNLRILKLAKIENSLQPYEMKYLDGKLLIQDRNNILAEKSENVTKEKP
                     TDAQLKDMEFGMRVVKNMKSNAIAIVKNGQTLALGCGQTSRIWALKNALENNKDKDFT
                     GAVLASDAFFPFDDCVTLAHEYGISAVVQPGGSIKDKDSIEACDKYDMVMVFTGIRHF
                     KH"
     gene            7073..8323
                     /gene="purD"
                     /locus_tag="KPD58_000561"
     CDS             7073..8323
                     /gene="purD"
                     /locus_tag="KPD58_000561"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBG0264348.1"
                     /translation="MKILVVGGGGREHAICWKLSKEKNVEKIYCAPGNAGIANVAECV
                     NIGDTNIEELLKFAKENEIGLTIVGPEVPLVMGIVDEFEKEGLRVFGPNKKCAQLEGS
                     KAFSKEFMIKHNIPTAKYKEYTNLEEAISEIDSFGYPVVIKADGLAAGKGVVIPENRE
                     DAIATLKEMMSDKKFGAAGDKIVIEEFLKGIETSILAFVDNDTIVPMASAKDHKKVNN
                     YEQGPNTGGMGTFSPSEIYTEELANKVKETVLEKTLEGFKKDGLNFKGILFVGLMITE
                     DGEKVLEYNVRFGDPETQSVLFRLETDLHEIMEAILDNKLKDIEINYSDDEAVCVMLT
                     SGGYPDSYEKGKIITGLENLDDDIVVFHSGTKMFDGNLVTNGGRVIGITAKSTTVKDA
                     AEKVYENIKKINFEGMHYRTDIGR"
     gene            8348..12154
                     /locus_tag="KPD58_000562"
     CDS             8348..12154
                     /locus_tag="KPD58_000562"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HBG0264349.1"
                     /translation="MLNTENKDSMVRRVLVEKREGFDLEAKALKKDLVESLHIDNIEN
                     LRILNRYDVEGISEEVYENAAKTIFSEPNLDVVYYEEIPKLNDERVFAIEFLPGQYDQ
                     RGDWAAQCVQIVNQGIRPAINTAKVYILSGKITDEEFSKIKDYCINPVDSREASLEKP
                     ETLKMETEIPTTVEVLDGFIDLDENGLRTFVSEKGLAMTLGDLQHVQKYFKDTEKRNP
                     TITEIKVLDTYWSDHCRHTTFMTEIENVKIEDGKFNDIVKEAYQMYLNSRDNVYVNRH
                     KDICLMDIATVAVKELKKNGKLNDLDESEEINACSINVDVEVDGKMEKYLVMFKNETH
                     NHPTEIEPFGGAATCLGGAIRDPLSGRSYVYQAMRVTGSADPRTTLEDTLPGKLMQRK
                     ITTEAAHGYSSYGNQIGLTTGQVAEVYDENFVAKRMEIGAVIAAAPKENVVRERPEAG
                     DVIVLLGGKTGRDGCGGATGSSKEHSEESILTCSAEVQKGDAPNERKIQRFFRNKEVA
                     QMIKRCNDFGAGGVCVAIGEIADSLDINLDLVPKKYDGLDGTELAISESQERMAVAIK
                     KENKDKFIQLAVEENLEATHVATVTDTGYLRMFWNGKAIVDINREFLDTNGVKQTTDV
                     HVTKVDEENTFFSSNEIVKDVKCASMKDKFTKVLSDLNVCSQKGLVEMFDNTIGGNTV
                     LMPFGGKYQATPTQGMVAKIPVLGGETNTSTIMTYGYNPKVGKWSPFHGALYAVVESV
                     CKLVAIGGNYSTTRLTFQEYFEKLGNNPEKWGKPFSALLGAFYAQSKFEIPAIGGKDS
                     MSGTFKDIEVPPTLVSFAVDTVDAKKVVSPEFKKADSKVVMLCVNKAENDVVDFEELK
                     RNLDKVRELIHGNKVLSTYALGFAGVGEAISKMAFGNKIGFKFSEEAEKAFTDDKLFE
                     ASYGNIVLELANDDLSMLEGYNYVVLGSTVKEASIFIKGEELALDELYKAHCSTLEPI
                     FPTKTEEVKSKIETISYISQGEAKKSSLSIATPRVFIPAFPGTNCEYDSARAFERAGA
                     NASIRVFKNLTYKDIEDSIDTIVNEIKSSQIIMLPGGFSAGDEPDGSGKFIATVFRNP
                     RVQEAINEFLTQKDGLMLGICNGFQVLIKLGLVPYGEIRVPSESAPTLTYNNIGRHQA
                     KIARTRISSNKSPWLAQTNVGDIHNIAISHGEGKFVASEDVMRELIANGQVATQYVDF
                     NNEATYDIEFNPNGSFYAVEGITSVDGRVFGKMGHSERIGEEVYKNIIGEKDQKIFES
                     GVKYFR"
     gene            12360..13232
                     /gene="rfbD"
                     /locus_tag="KPD58_000563"
     CDS             12360..13232
                     /gene="rfbD"
                     /locus_tag="KPD58_000563"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBG0264350.1"
                     /translation="MKILITGSNGQLGKELVNQLEAINQSINQPKYVILATTRSDLDI
                     SNQTNVDNFILHNKPDVVVNCAAYTKVDACEDNIEIAYKINALGVRNLAIASEKVNAK
                     LIHISTDYVFNGFSKYPYREDNKTEPNSVYGKSKLMGEKFVEQFSHKYFILRTAWLYG
                     NGNNFVKTMIKLSLENTEVNVVNDQFGTPTSTVDLAKIIIKIMETEHYGVYHATCEGE
                     CSWYDFAKKIFKLKNIDIKVNPIKSNEFKCKAPRPLYSVLDNFMLKLIGLNSFRKWEE
                     SIEEYLERYDINEK"
     gene            13341..14210
                     /gene="rfbA"
                     /locus_tag="KPD58_000564"
     CDS             13341..14210
                     /gene="rfbA"
                     /locus_tag="KPD58_000564"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBG0264351.1"
                     /translation="MKGIILAGGSGTRLYPITKCISKQTLPIYDKPMIYYPMSVLMLA
                     GIQDILIISTPRDIKAFEELFGDGSSLGLNITYAIQEKANGIAEAFIIGEEFIGNDRV
                     SLVLGDNIFYGYGFSERLENAVKRKGATIFGYHVSNPNSFGVVEFDKDFNVLSIEEKP
                     TVPKSNYAIPGLYFYDNDVVEIAKLVEPSDRGELEITSINSEYLKRGKLKVELLGRGM
                     AWLDTGTHKGLLDASNYVEVVQTRQGLYIACLEEIAYTKGYISREQLMKLAKPLIKTE
                     YGQYLLKLASEDM"
     gene            14259..14816
                     /gene="rfbC"
                     /locus_tag="KPD58_000565"
     CDS             14259..14816
                     /gene="rfbC"
                     /locus_tag="KPD58_000565"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBG0264352.1"
                     /translation="MGKINVIKTKIEGLYIVEPNVFYDERGYFMESYNKKDFIEQGLD
                     MCFIQDNESSSRKGVLRGMHFQVKYPQGKLVRVTEGEVFDVAVDLRKDSQTFGKWEGV
                     ILSDKNKKQFYIPEGFAHGFLVLSEKAVFSYKCTNYYYPEFENGFIWNDKQISIEWPL
                     KGIEEIFLSEKDKKQKSFKDICTRR"
     gene            14854..15837
                     /gene="rfbB"
                     /locus_tag="KPD58_000566"
     CDS             14854..15837
                     /gene="rfbB"
                     /locus_tag="KPD58_000566"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBG0264353.1"
                     /translation="MKKILITGGAGFIGSNFIHYMLNKYVDYFIVNLDLLTYAGNLET
                     LIEIQDNSNYKFVKGDISNREQVYKLFEEERFDIVVNFAAESHVDRSVENPDLFIKTN
                     IIGTQVLLDASIKYGVKRYHQISTDEVYGDLPIDRKDLFFTEQSSINPSSPYSASKAS
                     ADLLVSSYYRTYGLLTTISRCSNNYGPYHFPEKLIPLMILNALENKQLPVYGNGENVR
                     DWLHVYDHCTAIDLIIHKGNIGEIYNIGGHNERSNLEVVKMILNLLGKSEELISYVND
                     RPGHDLRYAIDATKIENELGWKAKYDFDLGIKETVKWYIENESWWKAVLSV"
     gene            15867..16535
                     /locus_tag="KPD58_000567"
     CDS             15867..16535
                     /locus_tag="KPD58_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBG0264354.1"
                     /translation="MNEISSLINILALTQLSGSNTTNQCNCTYNNSSGFDMIMMTLLK
                     ALSQNNQHTSNSYNLLSNSENLFNELDNVVNNTTSRFIDVDTKDKNVKLRIENAVDQA
                     SKKYNVDSNLIKAIIKVESDFNPNTVSSAGAKGLMQLMPENCRDLGVTDPFNIEQNID
                     AGTRHIKEYIDMFGGSIEMGLMAYNGGPGRMKSRGVESISDLYKMPKETQNYIPKVMK
                     YYRG"
     gene            16544..16972
                     /locus_tag="KPD58_000568"
     CDS             16544..16972
                     /locus_tag="KPD58_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HBG0264355.1"
                     /translation="MGINIDKLNISPSINMEVKKLKESNKFSDKLNSLKQSSSLEDIK
                     RNLESVKKAGERLVLTQNYGDISIYKNAVKEYLKSVVDNMYSLDKNSSFWEHQYYKNV
                     KIVDSKLEDMTSKLISEEKENIDIVSTVDMIQGLLIDMYR"
     gene            16996..17310
                     /locus_tag="KPD58_000569"
     CDS             16996..17310
                     /locus_tag="KPD58_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBG0264356.1"
                     /translation="MNSKKEDIYELNFGQLIEEKSGVIEENQNVFNAKVNVAVSIGNT
                     TETIKNILDLKEGSIIKLSKNVDEKLDIYANDRLFAYGESIITNERLSVRLSKIEDPD
                     VN"
     gene            17462..17737
                     /gene="flgM"
                     /locus_tag="KPD58_000570"
     CDS             17462..17737
                     /gene="flgM"
                     /locus_tag="KPD58_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HBG0264357.1"
                     /translation="MDIKNVSSNIVVNNYLKNTSGIKKVEEVKKINKTDKIELSKFAK
                     EMASVSNMSDENIRNKKVDEIKNALENGTYKIKPKEIAKKMIEDMRG"
     gene            17742..18158
                     /locus_tag="KPD58_000571"
     CDS             17742..18158
                     /locus_tag="KPD58_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HBG0264358.1"
                     /translation="MITDLKVIIYEEKNIISKMMELLKEQFDYIVEKDLENLNMLNPK
                     LEDISRELASIEIKRRQLFGDDISMSEVVENSNDGYLKEIYTDLKHILALAKNQQESN
                     DSLIKKELIFTKKMINFIKPADKQTTTYNSYGNIRK"
     gene            18176..19486
                     /gene="flgK"
                     /locus_tag="KPD58_000572"
     CDS             18176..19486
                     /gene="flgK"
                     /locus_tag="KPD58_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HBG0264359.1"
                     /translation="MAGLFSILNTANSGMNAHQKAIQTISHNISNLDTDGYSRQRTEF
                     ATNSPMYMPSLSNSIARGQLGTGVHVTDVTRARNSFYDYQFRAEAHKYGKTVSKYDYY
                     NTIETILNEPSDYGISAGIDDFFKGWESLSKDINSDSKKSLVVENASNLATLISESYK
                     KLDKLKEDIDSNIDTEIKQINDMLKSLEDLNKSIDIISGSGSTPNDLLDERDRILDNL
                     SFKLDFENSDVKNMLADGKLELNELKNPDGTWKTGISGTLQGLFEMHGKIDTYKSDLK
                     DVSDGLAKQINDVYNTSAGITVTDFFITSNVDGEDIIKINPAIKSNPNELKLTADEAS
                     KIAKLKDEKIDIGVAGGKVSTISDHYKAFAESIGLDSQKVNQDEANQRKIINNIDNSR
                     MSVSGVSLDEEMTELIKIQRSYQASAKVMSTAVQLLDVVINGII"
     gene            19505..20437
                     /gene="flgL"
                     /locus_tag="KPD58_000573"
     CDS             19505..20437
                     /gene="flgL"
                     /locus_tag="KPD58_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HBG0264360.1"
                     /translation="MRVSTGMMSSSYLNSLQDNLQRLDKVNRQVNTTKEINKLSDNPY
                     KAIKILNSKSEIKTMETYVENCKDTGNWLETTDTALDQLGKLLSDIKKTLVASGDGAY
                     SDDELKTLSNETNEKMKEIANALNATHEGKYIFSGSNTGVPPVECIDNADGSVSLKFN
                     SSLNLNKLNDSLSMDIAQGISVDYNVKLSSLGFDPTKTDPFEELNNISKKLANPDADA
                     IKELTTTCLGDMEQLIENTVNVRSIYGTKANTVEAMKEKNDDSLIQLKDILSQNEEID
                     YGEKLVQLKAAELTYQASLQTGGKLFNVSILDYI"
     gene            20453..20845
                     /locus_tag="KPD58_000574"
     CDS             20453..20845
                     /locus_tag="KPD58_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HBG0264361.1"
                     /translation="MMKVTLKKGILGFENLKEYELLDIEDNDSLKEFNSTEEEGIGFV
                     VVSPFEIINEYEIVLNQETIEKLEVKSPKDVVLFNIITLGQTFEESTVNMKAPIVINI
                     KTNFGMQIILQDDKYSIRHPLLRGDNGC"
     gene            20839..21051
                     /gene="csrA"
                     /locus_tag="KPD58_000575"
     CDS             20839..21051
                     /gene="csrA"
                     /locus_tag="KPD58_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HBG0264362.1"
                     /translation="MLVISRKKDEAVLIGESIEVKIVGVDGNNVKLAISAPNNISILR
                     KEIYEKVKSENIKATNKNIKILKSLK"
     gene            21073..21468
                     /gene="fliS"
                     /locus_tag="KPD58_000576"
     CDS             21073..21468
                     /gene="fliS"
                     /locus_tag="KPD58_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBG0264363.1"
                     /translation="MYGENPYNSYKQNAVFMASKEQLLLMLVDGAVKYTKIARAAILD
                     KNTQKAHRELVRVQDIFTELMVTLDQNAGQWAKDMYKVYDFVRYELSRANIRKDIQVI
                     DSVLPVIEQIKDTWHEADKKSKEERSRDK"
     gene            21468..21824
                     /gene="fliS"
                     /locus_tag="KPD58_000577"
     CDS             21468..21824
                     /gene="fliS"
                     /locus_tag="KPD58_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBG0264364.1"
                     /translation="MELNLAELDKLSKEELLLMLVDGTVKYTNISKEALLNNDYLKAH
                     NELVRVQNIFTELMTTLDQNAGQWAKDMYKVYEFIKSELAIADENKDIKIIDDILPIV
                     KQIRDTWHEVYNQLKI"
     gene            21845..23368
                     /gene="fliD"
                     /locus_tag="KPD58_000578"
     CDS             21845..23368
                     /gene="fliD"
                     /locus_tag="KPD58_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HBG0264365.1"
                     /translation="MSSISPIRVTGLSGNFDMEGIIEASMIRDKEKVDKAKQEQQIVK
                     WKQEIYRNVIQESKDLYDKYLSVNSPNSIVSEKAYSSTRITSSDESIIVAKGSAGAEK
                     INYQFAVSQMAEPAKFTIKLNSNEPIVRQFPPNASGASSLTIGDVNIPISEQDTTSTI
                     VSKINSLCADNDIKATYSEMTGELIISRKQTGSSSDINLRVIGNDNLAQQIANDNGIT
                     FINDAGGNKVASVYGKNLEADVTDEHGRVTHISKEQNSFNIDNIDYNVNSKGTAKLTS
                     ITDTEEAVKNMQAFVDDYNKLMDKVYGLVTTKKPKDYPPLTDAQKEDMTTEEIEKWEK
                     KAKEGILRNDDELRGFVEDIQSAFFGDGKNIIALRKLGINESENYNKKGQISFNADTF
                     SKALIDDSDKVYKTLAGYSSNYDDKGMFEKLKDIVYEYSGSSTSKLPKKAGIEKTASA
                     SENVYSKQIAEQERNISRLVEKMNDKEKRLYAKYSALESLLNQYSSQMNYFSQAQGN"
     gene            23371..23706
                     /gene="fliT"
                     /locus_tag="KPD58_000579"
     CDS             23371..23706
                     /gene="fliT"
                     /locus_tag="KPD58_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HBG0264366.1"
                     /translation="MDKLKEKLNLYKDISLQIINLIEKEEYIKISIKLGERQEIINSV
                     SEIDRNDFIQLYNRMELIEIDSRIRDILQGQLLEVKKELHEYKLTKQVNTMYYNLNRE
                     KVNIFNKKV"
     gene            23808..24680
                     /locus_tag="KPD58_000580"
     CDS             23808..24680
                     /locus_tag="KPD58_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HBG0264367.1"
                     /translation="MRVNTNVSALIANNQMGRNVNAQSKSMEKLSSGVRIKRAADDAA
                     GLAISEKMRAQIKGLDQAGRNVQDGISVVQTAEGALEETGNILQRMRTLSVQSSNETN
                     TAEERQKIADELLQLKDEVERISSSIEFNGKKLLDGSSTEIRLQVGANFGTNVAGTSN
                     NNNEIKVALVNTSSIMSKAGITSSTIASLNADGTSGTNAAKQMVSSLDVALKELNTSR
                     AKLGAQQNRLESTQNNLNNTIENVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLA
                     QANQQPQGVLQLLG"
     gene            24773..26914
                     /locus_tag="KPD58_000581"
     CDS             24773..26914
                     /locus_tag="KPD58_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG0264368.1"
                     /translation="MLLSIVMIVKNEEKILEKTLKSLTTLRNSIESELIIVDTGSTDD
                     TIKISKKYTEKVYFHNWNNDFSSMRNISISYAKGEWLLILDADEILIDSSTIVKFFKD
                     GLDKKFNSASIQLKNLYSYDKKLYGYCSVLRLFKNVDFRYHGKVHEQPIYKNPIFNNV
                     ADFEHYGYLFEDEEIRLNKVKRNEKLLFQELKENDNSPYTNYQLGKNFIILGKYQEAL
                     YYLEKSYELYSKLELAPGYLITSLVKTYLYLGKHKKCERLCLKYIKKDMNNIDIYYYI
                     AQAQVDLGKYENSIDSYKRYIYLLDNYEISTQANSLFSDTDTVGLRDEAIITLIKIYY
                     KLEKYEFVISEYNNIEDVEKKKNVYFSLFMSLYKLNRFEDIKNYYKEVSNSKIEKNSF
                     YRNIEEVINNIKEDEKEYVYKILCDIEGNYGILNQIRINKCVSIDKCKEILNEEKEII
                     YAYIIKIAFEEKIDLLDIFYEMEYVWIEKYLKYLLALERSFNLVLYKYIISRPNTSDM
                     EKIRVYKAISKVVLESISLSDEKYKEIFYFYIMYSYKYIRYIYSNLSDFELKKFVCND
                     LDKFTIEFKHLLDIKNKVNNANEKLEYIHSMRKLLNEYPFYNKIIKILIGELEENIKE
                     SEEFKDLKYSFIISIENMIETGKINDAKLLVDDYSKTFVDDVRILNIKGILYMFENKL
                     DKADFMLKKALSLDVENEDTIYNIEYLKSLR"
     gene            26938..28815
                     /locus_tag="KPD58_000582"
     CDS             26938..28815
                     /locus_tag="KPD58_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG0264369.1"
                     /translation="MNIPIVVNEKSKDIIKDWQYTCTNEDIKFTSIIILTYNQIEYTK
                     LCIESIRKFTPKNKYEIIIIDNNSSDGTVEWLKGQSDIKSVYNNKNLGFPKGCNQGIE
                     LATGENILLLNNDVIVTPNWLYNLNKALWSQDNIGAVGPITNACSYYQQIDVDYKSIE
                     DMFIFAHKINKSNINLWKYRLKLVGFCMLIKKTVLDNVGVLDELFTPGNFEDDDLSFR
                     MIESGYKLLLCQDTFIHHFGSVSFKENRNKYTDIMYINRRKFETKWGFNPEYSGMIRT
                     DLISKIDDSLEANINVLEIGCGIGATLLEIKNIYKNASIYGIEISESAGKLAKYSADV
                     LIANIEKVKLDYKKDFFDYIILGDVLEHLIDPWKIVNELKQYLKKDGYLVSSIPNIMH
                     VSVLRNLVLGNFTYEDSGILDKTHLRFFTLNEITKMFNDSNYIINSITATSMPLSEID
                     NSFIDTICKESCKEMRQQYESYQYIVKASKILDTKRYSSISMINLKYALMRIDNELDV
                     DSNLSYIFDNYNITEPCFVDDIEYLIDTFVINKATLLNRLGVEAFNRGLGDFSINMFM
                     TVLEMDRDDIDSIYNITSVLCELEEFDTAYNLIKNSSEKVQSESDIQEILCFIEGKKY
                     E"
     gene            28808..30922
                     /locus_tag="KPD58_000583"
     CDS             28808..30922
                     /locus_tag="KPD58_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBG0264370.1"
                     /translation="MNNDKICFITCVNDERLYEECLVYINSLDIPKNFSIETVALRGA
                     DSIASAYNKAMRETDSKYKVYLHQDTFIINKNFIHDILEIFKKNTDVGMIGVAGVKDM
                     PVNGIWWDSDKKAGKVYDSHTGKMDIYEFSNFEECFNEVMAIDGLIMITQYDVFWRED
                     KFDGWHFYDLSQSMEFRQNKYKVIVPKQNKPWCMHDSGVVNINNGYDEYRIKFVKEYI
                     NLDLYRENFYEFDKKKASTILKSNKHVMDCMKEELTEQLRLKNYKDVLGIVSKIAHFS
                     VFNHTGLYSCEECERALLECANNLPEIENTKLFTKKDLKRRKVLHVLSEGYSTGGHTR
                     LVKNWIEIDKDSVHSVVTTWQSDTLPDWLLKAINDSGGEVISLDLISDDYLKRAVALR
                     KIAYDWADIVILYVHMWDTIPVVAFGVDGKIPILHVNHYDSTFWLGTSIADIVIDFSE
                     TSQLITLDKRGIDKSEILPMPLKEKEVFSKSEVRKRYNLEDDVTIILTIASERKYNSV
                     NDINYFKIAKTLIENTDNTIIIVVGVDTENLYWKTLSNVTDNRVICFGTTANIDDFYK
                     ISDIYLESFMVGSHTSKLDAILYDLIPVKFKNEVSPIFTNMWNFIDKFDYKNIKEVID
                     MIRRYNTSEFDRDKIKAKQSEIKHFVIDKHCIEIKNYIDKIYNEVNHHKVKYDLYKND
                     EIEDYQLFVALYGYMVKNKNMS"
     gene            30931..31926
                     /locus_tag="KPD58_000584"
     CDS             30931..31926
                     /locus_tag="KPD58_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HBG0264371.1"
                     /translation="MNNIHINKFEKSCEKNWDYFVENKSINGTFLQTRNFLNYHEEGK
                     FTDHSLIICKGEEILAVVPACETYEDGERIFFSHLGSTFGGIIIRSGFNDIRHVNAIT
                     NKLEIYLQEQNFDKVYLKNTSRIFCKEDTSLLEYFLFKNNYMFYDELSMFLELSKYKE
                     DILANFKYSKRRDFKYSLKNNLTFKRLYSDEDIVCFFNLLSNCLDSKGAKVIHSVEEL
                     LEFKNNRLKDIVEFYAVYIDDIMICGSMVFNFQERVYHTQYLASNSTYNKYYPMIFLY
                     YNLIKDALERNFKYLSFGISTLNRGKYLDEGLATFKEGFGSSAICNRTYYKNINK"
     gene            32018..33250
                     /locus_tag="KPD58_000585"
     CDS             32018..33250
                     /locus_tag="KPD58_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HBG0264372.1"
                     /translation="MKRLMILGSIKYFENIVMSAKREGIYTIVCDNRIDTPAKKICDE
                     AIDVDVFDFDKIKNIAIDKKIDGILTGFTDSLMKPYVYIANELNLPCVISCNQLESVT
                     NKAVMKNYFKNNGIPTTDYCIIESLKDLEKIQGLNFPLVIKPVDGYGSRGVYFVSNIE
                     ELIEKFEYSKEASQDGQVIIEEFYESEEIQGLAWVHNGESHVFYIGDRELVNIHTGRA
                     GKPDRLIYPSKYCFQYEEEIKSIYQNITQAFGIKNGPLYVQMLVGAYGIKVSEVMPRL
                     PGGCDYMAISQITDLDIGSLFANFSVNNEVNFDEIKKYNMHLSKCVYALPIYIRPGTI
                     KKINNIDKIENLDYVTQFLLLVKEGDTIGLYEDERQDCGRVYGVANNVFDANIKKKCI
                     HEMIEILDENNENMIENF"
     gene            33270..34214
                     /locus_tag="KPD58_000586"
     CDS             33270..34214
                     /locus_tag="KPD58_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HBG0264373.1"
                     /translation="MKLITHEQIMSLNISQKQGYEWIKESFLTKKDCQLPPKISMVEE
                     GHVFYNVMPSLNHNHKIGGIKMVTRLPGRNPSLNSEIFLYNMEDAKLKAILDGNYITA
                     FRTAAVAALAIDTFAKKDFNTIAIMGLGVTASAIMNMLSYTLKDKNINIKLLKYKDQA
                     ESFVKKYEKNEQFSFEICDTHEELIVDSDVVISTVTYTDEYLSEFKWFKKGALLLPVH
                     VMGFGNVDYLFDKVYLDDYNHLKHLENIDRYKYSCEFYDVLSKQAKGRENDDERIISY
                     NIGLSIHDILFANKIYELCETLSCEEIELKEPKEKYWI"
     gene            34308..34715
                     /locus_tag="KPD58_000587"
     CDS             34308..34715
                     /locus_tag="KPD58_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxcM-like domain-containing protein"
                     /protein_id="HBG0264374.1"
                     /translation="MDKYNLIRFVSIGNEYIGNLVALESNKAIPFDIKRVYYIYDVPN
                     NVKRGFHAHKNLEQVLVCLNGSVKIKCYDGVKETIFNLHKNDVGLYIGTGVWHEMFDF
                     DEKTVLISIASEHYNEEDYIRDYKEFLKYLNNV"
     gene            34802..35893
                     /locus_tag="KPD58_000588"
     CDS             34802..35893
                     /locus_tag="KPD58_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HBG0264375.1"
                     /translation="MLVKFNNLLKQFQTHKEEYEQKIKDVMESGYYILGNEVKHFEEE
                     FANYVGSKYCVGLASGLDALIIAFRALGISKGDEVIVQSNTYIASVMGISINGATPVF
                     VEPNEYYNIDSSKIEEKINSKTKAILVVHLYGQASNMKDIQELCDKYNLYLIEDCAQS
                     HGAKYDGKMTGTFGDIGCFSFYPSKNLGAFGDAGAIVTSNEEIADKVKILRNYGSEKR
                     YYNKFVGYNSRLDEIQAGLLRVKLKYLNEIEIEKSKIVDRYLNEINNPKVILPKIENN
                     CTHVWHLFVLQVEKRDTFMEYLKKSGIESIIHYPIPPHLSEAYAYLGYKKGSFNIAEN
                     YSDKVVSIPLFIGMEDEEVSYIIKIINEY"
BASE COUNT        14706 a         3623 c         6570 g        11058 t
ORIGIN      
        1 ggtagtagtg gcgaagacac cgtccgaagt agcttggctt tgtaaccacc tctgttgtac
       61 tgtttaagta taaataagtg tgttagccac caagaatccc cttgctttag ctatggggag
      121 tgtgtcaata aaatacttta atgaaaaata gctaacttta attgtaggat attcttaata
      181 tatttgaaat aaataaaaat attgaaggat ataatattca attggtatat aaataataaa
      241 tgatagttaa attttacctt agaggctaat taggtgttta atcaaagtta atgcctttag
      301 aatacaaatg tattctaaag gcatttttac tatgtatttt catttatatt actaaacatt
      361 atctattttt atatacattt ctatgtattt tattttcatc aaatcggtct ataaactcat
      421 ttttttcttc actatatcca atagatataa gtgcataagg aactaatttt actggtaagt
      481 ctaaagtttg tctaacttta tttaccactt tatttttagg aaatactcct gcccaacaag
      541 cagctagacc ttgattagta acttctaata ataaattttg tgtgcaagca cctaaatctt
      601 gtatccattt accaggtttt aagaatttat ctctatttcc aagcactact atacataatg
      661 gtgcaaattc tatatgcttt gctctatgct gtgactttga gagctgtttc agcagttcct
      721 tatcatctac tataataaat tcccaaggtt gagcattttt tgatgaaggt gcttgcatag
      781 ctgattttag tatttcagtt attttatctt cttctatatt tttatctata tatgttctta
      841 ttgaatgtct attttttata acttctaaca tacagattct cctttaaata aaattatttc
      901 tatttatata atatcattac tttaaatttt agctattact tatataaaga ataaactttg
      961 tgttggtttt atctaagtaa aagtaacatt cagaactaaa aaacaagact ataaatccaa
     1021 agtattaatt aaaaaagaag taataaaaaa taatatacaa gatttaaaaa agagtattgg
     1081 gcttaaaatt taattagaaa accaattttg agataatctg ttttttattt taacaaaaaa
     1141 acataaataa agttcgatac atagcgaatg aaaaaattac aagaaatatt ttttttaaaa
     1201 aatttcaaaa aagagttgat aatatatgta tattaatata aaatttaagg tgtataattc
     1261 gtataataat acgaatgatt tgtagtgtaa aataacttat aaacggagag gggaagtcta
     1321 atgaaagtag cagtagttat gggttcaaaa tcagattatc caaaattaga agaagggata
     1381 aaactgcttg aaaaatatgg aatagaagtt gtagcaagag cattatcagc tcatagaaca
     1441 ccagaacaac tttcaatatt cctaaaagaa atagaagatg atactgatgt aataatagca
     1501 gcagcaggaa aagcagcaca cttaccaggt gtaatagctt cacaaacatt aattcctgta
     1561 ataggattac ctattaaatc atcaactatg gatggactag actcactttt atcaatagtt
     1621 caaatgccaa aaggaatccc agttgcaaca gttacaatag acttaggttt aaacgcagct
     1681 ttattggcat tacaaataat gactttaaaa tacccaaaat taaaagagga tttgaaatca
     1741 tatagagaag agatggcaca aaaggtgtta gaagacgaca agaatttaag ggggtagcag
     1801 aaatgttatt atatgaagga aaagcaaaac aagtatattc tacagataat gaaaatgaat
     1861 atgttgttta ttataaagat gatgcaacag catttaatgg agaaaaaaaa gcagaaatat
     1921 cttcaaaagg tatactaaat aataagatat caacaataat ctttgaaatg ttaaaagaaa
     1981 ataatataaa tactcacttt ataaaaagtt tatcagatag agaaatgtta gttaaaaaag
     2041 tagagatttt accattagaa gtaatagtaa gaaatattgc tgctggttct atctgtaaaa
     2101 gagttggtct tgaagaaggt gtagttttcg atgagccaat ttttgaaata agctacaaaa
     2161 atgatgctta tggtgaccca atgctaaatg atgattatgc agtggcaatg aagttagcaa
     2221 ctagagaaga gttaaaattc ttaagagaag aaactttaaa aataaacgag ttactaaaag
     2281 catttttctt aaaattaaac ctaaagttag ttgactttaa aatagaattt ggtaaagatt
     2341 ctgaaggaaa tataatattg gcagatgaag tatcaccaga cacttgtaga ctttgggatg
     2401 taaatacaaa tgaaaagtta gataaggaca gatttagaaa agaccttgga gaccttgtag
     2461 aaggttatac ggaagtactt tcaagaatga ataataaata ggaggcaaat gtatgtgtgg
     2521 agttttaggg atttactcta ataaagatgt aactaaagaa ctatattatt ctttatattc
     2581 tatgcaacac agggggcaag aaagctgtgg tttagctctt ttagatgatg gagaaattaa
     2641 gtacaaaaaa gatatgggat tagttggaga tgtttttaag gaaaatgaat tatctaagtt
     2701 aaaaggaaat atagggattg gacatgttag atattcaaca gctggaggaa gtcatgtttc
     2761 taactgtcaa cctttagttg gaagttgtag aaaaagacaa ttagccatag ctcataatgg
     2821 aaatctagtt aatgcaaatt atttaaaaga tatgctagaa gaagatggat atatgttcca
     2881 aactaattca gatacagaag tgattttata tatacttgct agatattaca aaggggatat
     2941 agtagaaagt ttaaaagtga caatggatta tataaaaggt gcatatgctc ttgtaataat
     3001 gagtcaagaa gaattggttg cagttagaga cccacatgga ttcagacctc ttgtacttgg
     3061 taaaaagggt gatgaatata tatttgcatc agaaaattgt gcaatagata tacttggcgg
     3121 agaagttata agagatgtag agccaggaga gataatagta gtcaaagatg gagaactaaa
     3181 atcatatttt tattcagaaa attataaacc agttaagaaa agttgtatat ttgaacacat
     3241 atactttgca agaaatgatg caactataga caatgtaaat gcatatgaat ttagaattaa
     3301 atgtggtgag agattagctc agaatgaaac tgtaaaggca gatatggtag ttccagttcc
     3361 agattcaggt tggccaggag ctattggtta tgcaaatgct agtggattaa aaataagtga
     3421 aggcttagtt aaaaatagat atgtaggaag aacatttata aaacctacac aagaagaaag
     3481 agaaatagca gtaaaaataa aattaaatcc tctttcaaca ataataaaag gaaaatctat
     3541 aattttagta gacgactcaa ttgtaagagg aactacatct aaacaattag taaaatcttt
     3601 aagagaagca ggagcaaaag aaattcatct tagaataact tctcctccag tagcttactc
     3661 ttgttactat ggaatagata ctccaaatcg ctctaaatta attgcatcaa gtaacaacgt
     3721 agaagaaatg agagaatata ttggatgtga tagtttaaaa ttcctagata tagaaggaat
     3781 gttagatgca acagaacata agtctacttt ctgtaaagct tgttttgatg gagaataccc
     3841 agttaagaaa attgataagg aggaactact ttcatgttaa catataaaga atcaggtgta
     3901 gatatagatg aaggaaatag agcagtagac cttataaaag gtaaaataaa aggaacttat
     3961 gatggaaatg tagttggaga tttaggtaac ttcagtggat tatacagttt aaaagatttt
     4021 gtaggtatga aagaaccagt tttgcttgca tctacagatg gtgttgggac taagttaaaa
     4081 atagctcaga tgatggacaa gcatgatact gttggaatag atttagttgc aatgtgcgtg
     4141 aatgatttaa tttgtcaagg agcaaaacca ttattcttcc ttgactatat agctcttggg
     4201 aaattagtgc ctgagcatat cgaaaaaata gttggtggaa ttgcagatgg ttgtaaaatg
     4261 tctggttgtg cactaattgg tggagaaact gctgaaatgc ctggaatgta tggagaagat
     4321 gactatgatt tagcaggatt ttctgtagga atagcagaca aagaaaagat tgtttctgga
     4381 aacaatgtta aatcaggaga tgtattggtt ggtatatctt caagtggagt acacagcaat
     4441 ggattttctt ttataagaaa gatattttta gaaacttaca attataaaat ggaacaatat
     4501 gttgaagaac taggaatgac agttggagaa gcacttttaa caccaactaa aatatatgtt
     4561 aaattagctt tagatgtatt agctaaacat gacataaaag ctatagctca cataactggt
     4621 ggagggctta tagaaaatat aacaagagtt atacctaaag ggttaggact agatatcaat
     4681 aaaaaatctt gggagaagcc tcctatattt aaaatgatag aaggttttaa tgctgttgat
     4741 gagagagaac ttcataagag ttttaacatg ggtattgggc tagttttaat agtagataaa
     4801 gaaaatgctg atgatgttgt aaactttata aataatagag aaaatgacaa tgctgcttat
     4861 gtagataaaa aatatagtga attattagaa gataaagctt atataattgg tgaagtagta
     4921 gatagtcatg aaggtgtaga attatgctaa atataggagt tttaatatct ggtggaggaa
     4981 caaatttaca agcagttata gatggtactg agtctggaga aataaaaggt caggttaaag
     5041 tagttatctc aagtaagcaa ggtgcttatg ggctagagag agctaaaaat cataacataa
     5101 aagcaatttg tgaaactgat gaagacaaga taatagaaat tcttaaggaa aataaaatag
     5161 atttagtagt tttggctggt tatttaaaaa taatcagccc aaagctggtt aacgagttta
     5221 gaaacaaaat gataaacata catccatcat taattccttc attttgtgga gctggattct
     5281 atggagaaaa agttcatcaa ggtgttatag attatggagc taaagtaact ggtgctacag
     5341 ttcattttgt agatgaaggt gctgatactg gaccaatcat aatgcaagat gtagttaagg
     5401 taaatcaaga tgatgatgca aagacacttg caaaaagagt gttagaagtt gaacatagga
     5461 ttttaaaaga aagtataagt cttttttgtg aaaataaatt aaaacttcaa ggtaggagag
     5521 tgtttataaa tgagtaagag agctttaata agtgtaactg acaagactgg tgtagtggaa
     5581 tttgctaaag agttaaataa attagattat gaaattatat ctacaggaaa tacatttaaa
     5641 acattaaaag aaaatggtgt taatgtaatg caagttgaag atgttacaaa ttttccagaa
     5701 atattagatg gaagagtaaa aacattaaat ccatatatac atggtggtat actttataaa
     5761 agagataaag aatctcatgt agaaactgta aatgaacaca aaatacattc aatagattta
     5821 gtagctgtaa atctatatga ttttgaagga actttaaaag ctggaaaaag tcatgatgaa
     5881 ataattgaaa atatagatat tggtggacca tctatgatac gttctgctgc caaaaactat
     5941 aaggatgtaa tagtagtagt agatataaaa gattatgatt ctataataga aaaattaaaa
     6001 acagatacaa tgacattaga agacagaaaa aaattatcat ataaagcttt ctcaactaca
     6061 ggaagatatg atgcgttaat atctagttat tttgctggag aagttggaga tacgtatcct
     6121 gacatattaa atttaacttt ccaaaaagaa caaactttaa gatatggaga aaatccacat
     6181 caaaatggtt tcttatattc acagtcaaat gcaaaaaatc caatcttaaa ttatgagcaa
     6241 ttaggcggta aagaactttc ttttaacaac ttaaatgatt tacatggatg tttagaagta
     6301 atgagagaat ttaaagatag tgaagaagta gtttcagtag ctataaaaca tgctaattca
     6361 tgtggggtag gtttaggaaa agatgctttt gaagcatata caaaatgtta tgaagcggat
     6421 aaggtttcaa tatttggagg aatagtagga ataacttcta ctatagataa agctacagca
     6481 gagaaattaa atgaaatatt cttagaaata gtagtagcat atgattttga accagaagct
     6541 ttagagatac ttaaacaaaa gaaaaatcta agaatattaa aattagctaa aatagaaaat
     6601 agtttacaac catatgaaat gaaatattta gatggaaaat tattgataca agatagaaac
     6661 aatatattag cagaaaaaag tgaaaatgta acaaaggaaa aaccaacaga tgcacaactt
     6721 aaggacatgg aatttggtat gagagtagta aaaaatatga aatcaaatgc aattgcaata
     6781 gtaaaaaatg gacaaactct agctttaggt tgtggacaaa catcaagaat ttgggcatta
     6841 aaaaatgctc ttgaaaataa caaagataaa gattttacag gagctgtttt agcgtcagac
     6901 gcatttttcc catttgatga ttgtgtaaca ttagctcatg aatatggaat atcagcagta
     6961 gttcaaccag gtggctctat aaaagataaa gattcaatag aagcttgtga caagtatgat
     7021 atggttatgg tatttacagg aataagacac ttcaaacact aaggaggata ttatgaagat
     7081 attggttgta ggcggtggcg gaagagagca tgctatatgc tggaaactaa gcaaagaaaa
     7141 aaatgtagaa aaaatttatt gtgcaccagg taatgcagga attgcaaacg tagcagaatg
     7201 tgtaaatata ggtgatacta atatagaaga acttttaaaa tttgccaaag aaaatgaaat
     7261 aggtctgaca atagtaggac cagaagtacc actagttatg ggaatagtag atgagtttga
     7321 aaaagaagga ttaagagtat ttggacctaa taaaaaatgt gctcaacttg aaggaagtaa
     7381 agctttttca aaagaattta tgataaaaca taacatacct actgcaaaat ataaagaata
     7441 tacaaactta gaagaagcta tatcagaaat agatagtttt ggatatccag tagtaataaa
     7501 agcggacgga ttagctgctg gaaaaggtgt tgtaatacct gaaaatagag aagatgctat
     7561 agcaacttta aaagaaatga tgtcagataa gaagtttgga gctgctggag ataaaatagt
     7621 aattgaagag tttttaaaag gaatagaaac ttcaattcta gcatttgtag acaatgatac
     7681 aatagttcct atggcaagtg caaaagacca taaaaaagta aacaactatg agcaaggtcc
     7741 aaacactggg ggaatgggaa ctttttcacc tagtgaaata tatacagaag aattagcaaa
     7801 taaagtaaaa gagacagtat tagaaaaaac tttagaaggc ttcaaaaaag atggacttaa
     7861 ttttaaaggt attttatttg ttggtcttat gataactgaa gatggagaaa aagtacttga
     7921 atataatgta agatttggag accctgagac acaatctgtt ttatttagat tagaaacaga
     7981 tttacatgag ataatggaag ctatactaga taataagtta aaagatatag aaataaacta
     8041 tagtgatgat gaagctgtgt gtgttatgct tacatcagga gggtatccag acagttatga
     8101 aaaagggaaa attataactg gtcttgaaaa cttggatgat gatatagtag tattccatag
     8161 tggcactaag atgtttgatg gtaatttggt taccaatgga ggaagagtaa ttggtataac
     8221 tgcaaagtca actacagtta aggatgcagc agaaaaagtt tatgaaaaca taaagaagat
     8281 aaactttgaa ggaatgcact atagaacaga cataggaaga taatttttct aaaatggagg
     8341 ataaggtatg ttgaatactg aaaataaaga ctcaatggtt agaagagtat tggttgaaaa
     8401 aagagaaggt tttgaccttg aggctaaagc tttaaaaaaa gacttagtag aaagtttaca
     8461 catagataat atagaaaatt taagaatatt aaatagatat gatgtagaag gaatatcaga
     8521 agaagtttat gaaaatgcag caaagacaat attctctgaa ccaaatttag atgtggtata
     8581 ttatgaagaa ataccaaaat taaatgatga aagagtattt gcaatagaat ttcttcctgg
     8641 tcaatacgac caaagaggtg attgggcagc tcaatgtgta caaattgtaa atcaagggat
     8701 aagacctgct attaatacag caaaagttta tatattatca ggcaaaataa ctgatgaaga
     8761 gttttctaaa ataaaagatt actgtataaa tccagtagat agtagagaag catctttaga
     8821 gaaaccagaa acgttaaaaa tggaaactga aataccaaca acagttgaag tattagatgg
     8881 atttatagat ttagatgaaa atggattaag aacatttgta agtgaaaaag gattagcaat
     8941 gactttagga gacttacaac acgttcaaaa atactttaaa gatacagaaa agagaaatcc
     9001 aactataaca gaaataaaag tattagatac ttattggtct gatcattgta gacatactac
     9061 ttttatgact gaaatagaaa atgtaaagat agaagatggt aagtttaatg atatagtaaa
     9121 agaagcgtat caaatgtatc ttaactcaag agacaatgta tatgtgaaca gacataaaga
     9181 tatatgttta atggatatag ccactgttgc agtaaaagaa ttaaagaaaa atgggaaatt
     9241 aaatgaccta gatgaaagtg aagaaataaa tgcatgtagt ataaacgtag atgttgaagt
     9301 tgatggtaag atggaaaaat atctagtaat gtttaaaaat gaaactcaca accatccaac
     9361 tgaaatagaa ccatttggtg gagcagctac ttgtttaggt ggagctataa gagacccatt
     9421 atcaggaaga agttatgttt atcaagcaat gcgtgttaca ggaagtgctg acccaagaac
     9481 gactttagaa gatactttac ctggaaaact tatgcaaaga aaaataacta cagaagctgc
     9541 tcatggatat agttcatatg gtaaccaaat aggtcttaca actgggcaag ttgcagaagt
     9601 atatgatgaa aactttgttg caaagagaat ggaaataggt gcagttatag cagcagcacc
     9661 aaaagagaat gtagtaagag aaagacctga agcaggagat gtaatcgttc ttcttggagg
     9721 aaaaacagga agagatggct gtggaggagc aactggttca tctaaagaac atagtgaaga
     9781 gtcaatctta acttgtagtg cagaagttca aaaaggagat gctcctaatg aaagaaaaat
     9841 acaaagattc tttagaaata aagaagttgc tcaaatgata aaaagatgta atgattttgg
     9901 agcaggtgga gtttgtgttg ctataggaga gattgctgat agcttagata taaacttaga
     9961 cttagttcct aaaaaatatg atggacttga tggaactgag cttgcaattt cagagtcaca
    10021 agagcgtatg gcagtagcca taaaaaaaga aaataaagat aaatttatac aattagcagt
    10081 agaagaaaac ttagaagcta cgcatgtagc tacagtaaca gatacaggat atttaagaat
    10141 gttctggaat ggaaaagcaa ttgtagatat aaatagagag tttttagata caaacggagt
    10201 aaaacaaact actgatgttc atgttactaa agtagatgaa gaaaatacat tctttagctc
    10261 taatgaaata gtaaaagatg ttaagtgtgc aagtatgaaa gacaagttta caaaagtact
    10321 atctgacctt aatgtttgtt ctcaaaaagg attagtggag atgtttgata atacaattgg
    10381 tggtaataca gtacttatgc catttggagg aaagtatcaa gcgactccaa cacaaggtat
    10441 ggttgctaag ataccagtac ttggaggaga gactaatact tctacaataa tgacttatgg
    10501 atacaatcca aaggtaggaa aatggagccc attccatggt gccttgtatg cagtagtaga
    10561 atctgtgtgt aaattggttg ctattggagg aaattatagt acaacaagac ttacattcca
    10621 agagtatttt gaaaaacttg gtaacaatcc agaaaaatgg ggtaaaccat tctcagcact
    10681 tttaggagca ttttatgcac aaagtaaatt tgagatacct gctataggtg gaaaagacag
    10741 tatgtctggt acatttaaag atattgaagt tccaccaaca ttggtttcat ttgctgttga
    10801 cacagtagat gcgaagaaag tagtatctcc agaatttaaa aaagcagact caaaagtagt
    10861 aatgttatgt gtaaacaagg cagaaaatga tgttgtagat tttgaggaat taaagagaaa
    10921 cttagataaa gtaagagaac ttatacatgg aaataaagtt ttatcaacat atgcattagg
    10981 atttgcaggt gtaggtgaag caataagtaa aatggctttt ggtaataaaa taggatttaa
    11041 gtttagtgaa gaggctgaaa aagcatttac agatgataaa ttatttgagg ctagttatgg
    11101 aaatatagta cttgaattag caaatgatga tttaagtatg ttagaaggat acaattatgt
    11161 tgtgcttgga agtacagtta aagaagctag tatatttatc aaaggtgaag aacttgcatt
    11221 agatgaatta tataaagcac attgtagtac tttagagcct atattcccaa ctaaaacaga
    11281 agaagtaaaa tcaaagatag aaactataag ttatatatca caaggagaag ctaaaaaatc
    11341 atcattaagt atagctactc caagagtatt tataccagca ttccctggaa caaactgtga
    11401 atatgactct gcaagagcat ttgaaagagc aggagctaat gcaagtataa gagtatttaa
    11461 gaatttaact tataaagaca tagaagattc tatagataca atagtaaacg aaataaaatc
    11521 ttctcaaata ataatgttac caggtggatt tagtgctggt gatgagccag atggttctgg
    11581 taaatttata gctactgtat ttagaaatcc tagagtgcaa gaagctataa atgaattttt
    11641 aactcaaaaa gatggactaa tgttaggtat ctgtaatgga ttccaagtat taattaaatt
    11701 agggttagtt ccatatggag aaataagagt tccaagtgaa agtgctccaa cacttactta
    11761 caataatatt ggtcgccatc aagctaagat tgctagaact agaatttcat ctaataaatc
    11821 tccttggtta gctcaaacta atgttggaga tattcataat atagcaattt ctcatggaga
    11881 aggtaagttt gttgccagtg aagatgttat gagagaatta attgctaatg gacaagtagc
    11941 aactcaatat gttgacttta ataatgaagc tacttatgat attgagttta atccaaatgg
    12001 ttcattctat gctgttgaag gtataactag tgttgatggt agagtgtttg gtaaaatggg
    12061 acattctgaa agaattggtg aggaagtata taagaatatt ataggtgaaa aagaccaaaa
    12121 gatatttgaa tctggagtta aatattttag ataaagcttt gatgtttaat tgatagctag
    12181 aatatataat ttagatattt agatttaaag agatgtaata atatgatgat aatcatttaa
    12241 tattacatct cttttttatt tatctaagaa ataataagtt caacctaagt taaaatacaa
    12301 gctgtgttat aattaattat taagtaaaat tgaatattta ttggagggca acataattaa
    12361 tgaaaatatt aataacaggg tctaatggtc aattaggaaa agaattggtt aaccaattag
    12421 aagcaatcaa tcaaagtata aaccaaccaa aatatgttat attagctaca acaagaagtg
    12481 atttggacat atccaatcag actaatgtag ataattttat tttgcataat aaacctgatg
    12541 tagttgtaaa ctgtgcagca tatacaaagg ttgatgcatg tgaagataat atcgaaattg
    12601 catacaaaat aaatgcttta ggagtgagaa atctagcaat agcatcagaa aaagttaatg
    12661 ctaagcttat tcacatatca actgactatg tattcaatgg tttttctaaa tatccatata
    12721 gggaagacaa taaaactgaa cccaattctg tatatggaaa aagtaaactt atgggagaaa
    12781 aatttgttga acaattctca cataagtact ttatattgag aacagcatgg ttatatggta
    12841 atgggaataa ttttgtaaag accatgataa aactttcttt agaaaataca gaggttaatg
    12901 tggttaatga ccagtttgga actccaacaa gtacagtaga cttagcaaaa attataataa
    12961 aaatcatgga aacagaacat tatggagttt atcatgctac atgtgaaggt gagtgtagtt
    13021 ggtatgattt tgccaaaaag atatttaaac taaaaaatat agatataaaa gtaaatccaa
    13081 ttaagagtaa cgaatttaaa tgtaaggctc caagaccact atattctgta ttggataatt
    13141 ttatgttgaa gttaattggt ttaaattctt ttagaaagtg ggaagaatcc attgaagaat
    13201 atttagaaag atatgatata aatgaaaagt aaatgtatta ttttgagaaa agaaaataag
    13261 atattataaa aaatataaat atttctttta tttatccgat aatcataata tgacaaaaac
    13321 aaatttaagg atggaaaaag atgaaaggaa ttattttagc aggagggtct ggtacaagac
    13381 tatatcctat aacaaaatgt atatcaaaac aaacacttcc aatatacgat aaaccaatga
    13441 tatattaccc tatgtcggtt cttatgttgg cagggataca agatatatta ataatatcta
    13501 caccaagaga tataaaagca tttgaagaat tatttggaga tggaagttca ctagggctta
    13561 acataacata tgcaattcag gagaaagcaa atggaattgc agaagcgttt ataataggag
    13621 aagaatttat aggaaatgac agagtgtctc ttgtattagg ggataatata ttttatggat
    13681 atggatttag tgaaaggttg gagaatgcag ttaaaagaaa aggagcaact atatttgggt
    13741 atcatgtaag taatccaaat tcgtttggag ttgtagaatt tgataaggat tttaatgtat
    13801 tgtcaataga agaaaaacct acagttccta aatctaatta tgctatacca ggattatatt
    13861 tttatgataa tgatgttgta gagatagcaa agctagttga accgtcagat agaggtgaat
    13921 tagaaataac ctctataaac agtgaatatc taaaacgagg aaaactaaaa gtggaacttc
    13981 taggaagagg tatggcttgg cttgatactg gtacacataa aggtctattg gatgcttcta
    14041 attatgttga agtggttcaa acaagacagg gactgtatat agcatgttta gaagagattg
    14101 catatacaaa aggatatata agtagagaac aattaatgaa actagccaag ccactgataa
    14161 aaactgaata tggtcaatat ctattaaaat tggctagtga agatatgtaa tatggtagat
    14221 aaaatttatt ataaatttta ttaagggagt ttattataat gggaaaaatc aatgttataa
    14281 agactaaaat agaaggatta tatatagtcg aaccaaatgt attttatgat gaaagaggat
    14341 attttatgga aagctataat aaaaaggatt ttatagagca gggtcttgat atgtgcttta
    14401 ttcaagataa tgagtctagc tcaagaaaag gcgttttaag aggaatgcat tttcaggtta
    14461 aatatcctca aggaaaatta gtcagagtta cagagggaga agttttcgat gttgcagttg
    14521 atttaaggaa agattcacaa acatttggga aatgggaagg tgtaatattg tctgataaga
    14581 ataaaaaaca attttatatt ccagaagggt ttgcacatgg atttttagtt ttatcagaaa
    14641 aagcagtatt tagttacaaa tgtacaaatt attattaccc agaatttgag aatggcttta
    14701 tatggaatga caagcaaata tctattgagt ggcctttaaa aggaattgaa gaaatatttt
    14761 tatcagaaaa ggacaaaaaa caaaaaagtt ttaaagatat ttgtacaaga cgttaataaa
    14821 aatttattag aattaacatt taggagaaaa aatatgaaaa aaatactgat aactggagga
    14881 gcaggattta ttgggagtaa ttttatacat tatatgctta acaagtatgt agattatttt
    14941 attgttaacc tagacttgtt gacttatgct ggtaatttag agacattaat agaaatacaa
    15001 gacaattcca attataaatt tgttaaagga gatatttcaa atagggagca ggtgtataag
    15061 ctatttgaag aagaaaggtt tgatatagtt gttaactttg ctgctgaatc tcatgtagat
    15121 aggtcagttg agaatcctga tttatttatt aaaacaaaca ttataggaac acaagtactt
    15181 ttagatgcat ctataaaata tggagttaaa aggtatcatc aaatatctac agacgaagtg
    15241 tatggtgatt taccaattga tagaaaggat ttattcttta cagaacaatc ttctatcaat
    15301 ccaagttctc catattcagc ttctaaagca tcagcagatt tattggtaag ttcttattat
    15361 agaacatatg gattgttaac aactatatct aggtgttcca acaattatgg gccatatcat
    15421 tttcctgaaa aattgattcc tcttatgatt ttaaatgctt tggaaaacaa acaattgcca
    15481 gtatatggga atggggaaaa tgtaagagat tggttacatg tatatgacca ttgtactgct
    15541 atagacttaa taatacataa aggaaatata ggggagattt acaatatagg aggtcataat
    15601 gaaagaagta atctagaagt agtaaaaatg atattaaatc ttttaggtaa atctgaagag
    15661 ttaatatcct atgtaaatga tagacctggt catgatttaa gatatgctat tgatgcaact
    15721 aaaatagaga atgagttagg ttggaaggca aaatatgact ttgatttggg tataaaagaa
    15781 actgttaagt ggtatataga aaatgaatct tggtggaaag ctgttttatc agtataactt
    15841 taaaatttag aaaggggcgt tttatattga atgagataag ttcactaatt aatatattag
    15901 cattgactca attatctggt agtaatacta caaatcaatg taattgtaca tataataatt
    15961 catctggatt tgatatgatt atgatgacct tgttaaaagc attatcacaa aataatcaac
    16021 atacttctaa tagctacaat ttattgtcta attctgagaa tttatttaat gaacttgata
    16081 atgtagtaaa taataccact agtagattta tagatgttga tacaaaagat aaaaatgtaa
    16141 agttgagaat tgaaaatgct gtagatcaag catcaaaaaa atataacgta gattcaaatc
    16201 ttataaaagc aataattaaa gttgaatcag attttaatcc aaataccgtt tcttctgctg
    16261 gtgctaaggg tcttatgcaa cttatgccag aaaactgcag agacttagga gttacagacc
    16321 cttttaatat agaacagaat atagatgctg gaactagaca tattaaggaa tacattgata
    16381 tgtttggagg gagcatagaa atgggattga tggcatataa tggtggtcct ggaagaatga
    16441 aaagtagagg agtagaatca atctctgacc tgtataaaat gccaaaagaa acacaaaatt
    16501 atattcctaa ggttatgaaa tattatagag ggtaggcttt attatgggaa taaatataga
    16561 taagctaaac ataagtccaa gtataaatat ggaggttaaa aaactaaaag agagtaataa
    16621 attttctgac aaattaaact ctttaaaaca atcttcttca ttggaagata taaaacgaaa
    16681 tttggaaagt gtaaaaaagg caggagaaag gcttgtactt actcaaaact atggcgatat
    16741 tagtatatat aaaaatgcag taaaagaata cctaaaatct gttgtagata atatgtattc
    16801 acttgataaa aatagtagct tttgggagca tcagtattat aagaatgtaa agattgttga
    16861 tagtaaactt gaggatatga ctagtaagtt aattagtgaa gaaaaagaaa acatagatat
    16921 tgtttctact gttgacatga tacaaggctt attgatagat atgtatagat aaggttatac
    16981 aaggagaaaa attttatgaa tagcaaaaaa gaggatatat atgaattgaa ttttgggcaa
    17041 ttaatagaag aaaaatctgg tgttatagaa gaaaatcaaa atgtttttaa tgcaaaagta
    17101 aatgtagctg tttctatagg aaatacaaca gaaacaataa aaaatatatt agatttaaaa
    17161 gaaggctcta taataaaatt aagtaagaat gtggatgaaa aattagatat atatgcaaac
    17221 gatagattgt ttgcttatgg agaaagcatt attacaaatg agaggttatc tgtaagatta
    17281 tctaaaatag aagatccaga tgtaaattag cattttaaat agtatccctt taggctattc
    17341 tgtatgagtt tagctaaggg gatattttta taaaaaattt aaaatatgtg tgaattatat
    17401 aaatattttt attttatgag cgataactaa attataagta tgtaattttg gaggtgaaac
    17461 tatggatatt aaaaatgtaa gttcaaatat tgtagtgaat aattatttaa agaatacatc
    17521 tggtataaaa aaagtggaag aggttaaaaa aattaataaa acagacaaga tagagttatc
    17581 taaatttgca aaggaaatgg caagtgtgtc aaatatgagt gatgagaata ttagaaataa
    17641 aaaagtcgat gaaataaaaa atgcattaga aaatggaact tacaaaataa aaccaaaaga
    17701 aattgcgaaa aaaatgatag aggatatgcg aggataaagt catgattaca gacttaaaag
    17761 ttataatata tgaagaaaaa aatataatta gtaagatgat ggaattatta aaagaacaat
    17821 ttgattatat agttgaaaaa gacttagaaa atttaaatat gttaaatcct aaactagaag
    17881 atattagcag ggagcttgca tccatagaaa taaagagaag acaacttttt ggtgatgata
    17941 tttcaatgag tgaagttgtt gaaaatagta atgatggata cttaaaagaa atatatacag
    18001 acttgaaaca cattcttgct ttagctaaaa atcaacaaga atcaaatgat tctctaataa
    18061 agaaagaact tatttttacg aaaaaaatga ttaatttcat aaaaccagct gataagcaaa
    18121 ctacaactta taattcttat ggaaatatta gaaaataaat attaggagga gaaatatggc
    18181 aggattattt tccattttaa atacagctaa ttcaggaatg aatgcgcacc aaaaagcaat
    18241 acagacaatt tctcataata taagtaatct agatacagac ggttattcta ggcaaaggac
    18301 cgagtttgcc acaaatagtc caatgtatat gcctagtttg agtaattcaa ttgcaagagg
    18361 acaattaggg actggagtac atgtaacgga tgtaacaaga gctagaaata gtttttatga
    18421 ttaccaattt agggcagaag ctcataagta tggtaagaca gtttcaaaat atgattatta
    18481 caatactata gaaactatac taaatgaacc atcagactat ggaatatctg caggtataga
    18541 tgattttttt aaaggatggg agtcattatc aaaggatata aatagtgaca gtaaaaaaag
    18601 tctagtggta gaaaacgcat ctaatttagc aactttaata agtgaaagtt ataagaagct
    18661 tgataagtta aaagaagata tagattctaa tatagacact gagataaaac aaataaatga
    18721 catgttaaaa agtcttgaag atttaaataa atctattgat attatatctg gttctggctc
    18781 tacaccaaat gatttacttg atgaaagaga tagaatttta gataacttga gttttaaact
    18841 agactttgaa aatagtgatg ttaaaaatat gcttgcagat ggtaaattag aacttaatga
    18901 attaaaaaat ccagatggaa cttggaaaac aggtatatca gggaccttac aaggcttgtt
    18961 tgaaatgcat ggtaagattg atacatataa aagcgattta aaagatgtat ctgatggatt
    19021 agctaaacag ataaatgatg tttataatac gtctgcagga ataacagtaa cagatttttt
    19081 tataacttca aatgtagatg gtgaagatat tataaagata aatccagcta taaagagtaa
    19141 cccaaatgaa ttaaagctta ctgctgatga agcttcaaaa attgctaagt taaaggatga
    19201 aaaaatagat attggggtgg ctggtggaaa agtaagtaca ataagtgacc actataaagc
    19261 ctttgcagaa tcaatcggtt tagattctca aaaagtaaat caagatgaag caaatcaacg
    19321 taaaattata aataatattg ataactcaag aatgagtgtt tcaggtgtat ctttggatga
    19381 agaaatgaca gagcttataa aaatacaacg ttcatatcaa gctagtgcaa aagttatgtc
    19441 aacagctgtg caactattgg atgttgtaat aaatggaata atttaataga agggggaatt
    19501 tataatgaga gtcagtacag gtatgatgtc tagcagctat ctaaactcac tacaagataa
    19561 tctacaaaga ttggataagg ttaatagaca agttaataca acaaaagaaa taaacaagct
    19621 atcagataat ccatataaag caataaagat attaaactca aaaagtgaaa taaagactat
    19681 ggagacttat gttgagaatt gtaaagatac agggaattgg ttagaaacta ctgatactgc
    19741 attagatcag cttggaaaat tattatcaga cataaagaaa actttggtgg caagtggtga
    19801 tggtgcatac tcagatgatg aattaaagac tcttagtaat gaaactaatg aaaaaatgaa
    19861 agaaattgca aatgctttaa atgcaacaca tgaagggaag tacatatttt caggttcaaa
    19921 tacgggggta ccacctgttg aatgtataga taatgctgat ggttcagtga gtttaaagtt
    19981 taattctagt ctaaatctta ataaattaaa tgactctttg agtatggata tagctcaagg
    20041 aataagtgta gattataacg ttaaattgag tagccttgga tttgacccaa caaaaactga
    20101 cccatttgaa gagttaaata acatatcaaa aaaattagca aatccagatg ctgatgctat
    20161 aaaagagctt acaacaactt gtttgggcga tatggaacaa ctaatagaaa atacagtaaa
    20221 tgttaggtct atatatggga caaaagcaaa tacagtagag gctatgaagg aaaaaaacga
    20281 tgatagtttg atacagttaa aggatatttt gtcacaaaat gaagaaatag attatggtga
    20341 aaaacttgtt caattaaagg cagcagagtt aacttatcaa gcttcactac aaacaggagg
    20401 aaagttattt aatgtatcta tattagatta catatagtaa aataacgcag atatgatgaa
    20461 ggttacatta aaaaaaggta tattaggttt tgaaaattta aaagaatatg agcttttaga
    20521 tatagaagat aatgattctt taaaagaatt taattctact gaagaagaag gtataggatt
    20581 tgttgttgta tctccatttg agattatcaa tgaatatgaa atagttttaa atcaggaaac
    20641 aatagaaaaa ctagaagtga aatctccaaa ggatgttgta ctatttaata taattactct
    20701 tggtcagaca tttgaagaat caactgtgaa catgaaagct cctatagtga taaatataaa
    20761 aactaatttt ggaatgcaaa taattttaca ggatgataaa tattcaataa ggcatccatt
    20821 attgagaggt gataatggat gctagtaatt tcaagaaaaa aagatgaagc agtacttata
    20881 ggagaaagca tagaagtaaa aattgtaggg gtagatggaa acaatgtgaa actagctata
    20941 tcggcgccta acaatataag tatattaaga aaagaaatat acgaaaaagt aaagagtgag
    21001 aatataaaag ctacaaataa aaatataaaa attttaaagt cattaaaata ataagcttgg
    21061 aggaagtaat atatgtatgg agaaaatcct tataattctt ataaacaaaa tgctgttttt
    21121 atggcatcaa aggaacaatt gttacttatg cttgttgatg gagcagtaaa atataccaaa
    21181 atagccagag cagctatctt agataaaaat acacagaaag cgcatagaga acttgtaaga
    21241 gttcaggata tatttacaga actaatggtt acattagacc aaaatgcagg acaatgggca
    21301 aaggatatgt acaaagttta tgattttgta agatatgaac tttcaagagc caatattaga
    21361 aaagacatcc aggtaataga tagtgtgttg cctgtaatag aacaaattaa agatacatgg
    21421 catgaagctg ataaaaaaag taaagaagaa agaagtaggg acaaataatg gaattaaatt
    21481 tagctgaatt agataagtta tcaaaagagg aattactatt gatgcttgta gatggcacag
    21541 taaaatatac caatatatcc aaagaagctc ttttaaataa cgactatttg aaagcacata
    21601 atgaacttgt aagagttcaa aatatattta cagaactaat gactacatta gaccaaaatg
    21661 caggacaatg ggcaaaggat atgtataaag tttatgaatt tataaaatct gaattagcaa
    21721 tagctgatga aaataaagat attaaaataa ttgatgatat attgccaata gtaaaacaaa
    21781 ttagagatac atggcatgaa gtttacaatc aactaaaaat ataattagat ggagggataa
    21841 aggtatgtca agtataagtc ctataagagt tacaggtctt tcaggaaatt ttgatatgga
    21901 aggcataatc gaagctagta tgattagaga caaggaaaaa gttgataaag caaaacaaga
    21961 acaacaaatc gttaaatgga agcaagaaat atatagaaat gttatacaag aatcaaaaga
    22021 tctttatgat aaatatctaa gcgtaaattc tcctaatagt atagtaagtg aaaaagcata
    22081 ctcttctaca agaataacca gttctgatga aagtattata gtagcaaaag gttcagctgg
    22141 tgcagaaaaa ataaattatc aatttgcagt ttctcaaatg gctgaaccag caaaatttac
    22201 tattaaacta aattcaaatg aacctattgt tcgacaattc cctccaaatg ctagtggagc
    22261 tagttcttta actataggag atgtaaatat accaatatct gaacaagata ctacaagtac
    22321 tattgtaagt aagataaact cactttgcgc agataatgat ataaaggcta cttatagtga
    22381 gatgacaggt gaattgatta tttcaagaaa acagactggt tcgtcatcag acattaattt
    22441 aagagtaatt ggaaatgaca atttggctca gcaaattgcc aatgataatg gtatcacatt
    22501 tataaatgat gctggtggaa acaaagtggc aagtgtatat ggaaagaatc tagaagctga
    22561 tgtaactgat gaacatggaa gagtaactca tataagtaaa gaacaaaatt catttaatat
    22621 agataatatt gactataatg taaattcaaa aggaactgca aagttgactt ctatcactga
    22681 tactgaagaa gctgttaaaa atatgcaagc atttgtggat gattataata aactgatgga
    22741 caaggtctat ggtttagtta ctactaaaaa gccaaaagat tatccacctc ttacagatgc
    22801 ccaaaaagaa gatatgacaa ctgaagaaat agaaaaatgg gaaaagaaag ctaaagaagg
    22861 tatacttaga aatgatgatg agttaagagg ttttgttgaa gatattcagt ctgcattttt
    22921 tggagatgga aaaaatatta ttgcattaag aaaactaggt atcaatgaaa gtgaaaatta
    22981 caataaaaaa ggtcaaatat catttaatgc agatactttt tcaaaggctc ttatagatga
    23041 tagtgataag gtatacaaaa cactagcagg ttattcttcg aattatgatg ataagggaat
    23101 gtttgaaaag ctaaaagata ttgtatatga atattctgga agttcaactt ctaaacttcc
    23161 taaaaaagca ggtatagaaa aaactgcttc tgctagtgaa aatgtatatt caaaacaaat
    23221 tgcagagcaa gaaagaaata taagcaggtt agttgaaaaa atgaatgata aagagaaaag
    23281 actttatgct aaatattcag ccttagaatc tttgttgaat cagtattctt cccaaatgaa
    23341 ttatttctca caagcacagg gtaattaatt atggataaat taaaagaaaa attaaattta
    23401 tataaagata tatctttaca aattataaat ttgattgaaa aggaagaata tataaagata
    23461 agtattaagt taggtgaaag acaagagatt ataaatagtg taagtgaaat tgatagaaat
    23521 gattttatac agctttataa tagaatggaa ttaattgaaa ttgatagcag gataagagat
    23581 attttacaag gacaattatt agaagtaaaa aaagaattac atgagtataa gcttactaaa
    23641 caagtgaaca caatgtatta taacttaaat agagaaaaag taaatatttt taataaaaaa
    23701 gtataaagtt atgtaccatc ttgtccgatt atataaatga agaattaata aaaaagatta
    23761 aaagtagaaa tgacaaggat gtcaactata ctaaggaggg taaaataatg agagttaata
    23821 caaatgtaag tgctttgata gcaaataacc aaatgggaag aaacgtaaat gcacaaagta
    23881 agtctatgga gaagctatct tctggtgtta gaattaagag agctgctgac gatgctgctg
    23941 ggcttgctat atctgaaaaa atgagagctc aaataaaagg tttagaccaa gcaggaagaa
    24001 acgttcaaga tggtatatct gttgtacaaa cagcagaagg tgcattagaa gaaactggta
    24061 acatactaca aagaatgaga actttatcag tacaatcttc aaatgaaact aatactgctg
    24121 aagaaagaca aaagatagct gatgagttac tacaattaaa agatgaagtt gaaagaatat
    24181 caagctcaat tgagtttaat ggtaagaaat tacttgatgg aagttctaca gaaataagat
    24241 tacaagttgg agcaaacttt ggaacaaatg ttgcaggaac atctaacaac aacaatgaaa
    24301 taaaagttgc tctagtaaat acttcaagta taatgagtaa agcaggtata acaagttcta
    24361 caatagcaag cttaaatgca gatggaactt ctggaacaaa tgcagctaaa caaatggtat
    24421 ctagcttaga tgtagctctt aaagaattaa atacatcaag agcaaaatta ggggcacaac
    24481 aaaatagact agaatcaact caaaacaact taaataatac tatagaaaat gttacagcag
    24541 ctgaatcaag aataagagat acagatgttg cttcagaaat ggttaactta tctaaaatga
    24601 atatattagt tcaagcatca cagtcaatgc ttgctcaagc taatcaacaa ccacaaggag
    24661 ttttacaatt attaggataa aataattttg tatattggat atagaagaaa gggtaaggct
    24721 ttgctttact cctttctttt tttacaaata tacttttgaa aggaaaattt atatgttact
    24781 tagtatagtt atgattgtta aaaatgaaga aaaaatactt gaaaaaactt taaaatcatt
    24841 aaccacatta agaaattcta tagaaagtga acttataata gtcgatacag gttctacaga
    24901 tgatactata aaaatatcta agaagtacac ggaaaaagtg tattttcata attggaataa
    24961 tgatttttct agtatgagaa atatatcaat atcctatgct aaaggtgaat ggttacttat
    25021 attagatgca gatgaaatac taattgattc ttcaactatt gtaaaatttt ttaaagatgg
    25081 tcttgataaa aaatttaatt ctgcttcaat acaattaaaa aatctttact catatgataa
    25141 aaaattatat ggatattgtt cagtgcttag actttttaaa aacgttgatt ttagatacca
    25201 tggtaaagta catgaacaac caatatacaa aaaccctata tttaataatg ttgctgattt
    25261 tgaacattat ggatatttgt ttgaagatga agaaataaga ttaaataagg tgaaaagaaa
    25321 tgaaaaacta ttatttcaag agttaaaaga gaatgataat tctccatata caaactatca
    25381 attagggaaa aattttatta tattaggaaa atatcaggag gctttatact atcttgagaa
    25441 atcttatgaa ttatattcta aactagaatt ggcacctggt tacttgataa caagccttgt
    25501 aaaaacatat ttatatttag gaaaacataa gaagtgtgaa agattgtgct taaagtatat
    25561 taagaaagac atgaataata ttgatatata ttattatatt gcacaagcac aagtagattt
    25621 aggaaagtat gaaaatagta tagattcgta taagagatat atttacttgt tagataatta
    25681 tgaaataagt acacaagcaa atagtttatt ttcagacact gatactgtag ggcttagaga
    25741 tgaggctatt attacactga taaagattta ttataagctt gaaaaatatg agtttgtaat
    25801 ttcagagtac aataacattg aagatgtgga aaagaaaaag aatgtgtact ttagcttatt
    25861 tatgtcccta tataaattga atagatttga agatataaaa aattattata aagaagtttc
    25921 aaattctaaa attgaaaaaa atagttttta tagaaatata gaagaagtta taaataatat
    25981 taaagaggat gaaaaagagt atgtatataa aatcctttgt gacattgaag gtaattatgg
    26041 cattttaaat caaataagga taaataaatg tgtatctata gataaatgta aagaaatact
    26101 aaatgaagaa aaggaaataa tttatgcata tattataaaa atagcttttg aagagaagat
    26161 tgatttacta gatatttttt atgaaatgga atatgtatgg atagagaaat atctaaaata
    26221 tcttttagca ttagaaagaa gttttaattt agtactatat aagtacataa ttagtcgtcc
    26281 aaatacatca gatatggaaa agataagagt atataaagct atatctaaag tagttttaga
    26341 aagtataagc ttaagtgatg aaaaatataa ggagattttt tatttttata taatgtattc
    26401 ttataagtat ataagataca tatatagcaa tttaagtgat tttgaattaa aaaaatttgt
    26461 ttgcaatgat ttagataagt tcactataga atttaagcat ctacttgaca ttaaaaataa
    26521 agtaaataat gctaatgaaa aattagagta tatacatagt atgaggaaat tactaaatga
    26581 gtatcctttt tataataaaa taataaaaat acttataggt gaattagaag aaaatataaa
    26641 agaaagtgaa gaatttaagg atttaaaata tagttttatt attagtatag aaaatatgat
    26701 tgaaactggc aaaataaatg atgcgaaatt attggtagat gactattcaa aaacatttgt
    26761 ggatgatgta aggattttaa atataaaggg aattttatat atgtttgaaa ataagcttga
    26821 taaagctgat tttatgttaa aaaaggcatt atcattagat gtagaaaatg aagatactat
    26881 atataatata gaatacttaa aaagtttaag ataagtcaaa aataggagaa ttgaagaatg
    26941 aatataccaa tagtggttaa tgaaaaatct aaagacataa taaaagattg gcaatataca
    27001 tgtacaaatg aagatataaa atttacaagc ataataatat tgacatataa tcaaatagaa
    27061 tatactaaat tatgtattga aagtattagg aaatttaccc caaagaataa atatgaaata
    27121 ataataattg ataataactc ttctgatgga acagttgagt ggttaaaggg acaatctgat
    27181 ataaaatctg tgtataataa caaaaactta ggatttccaa aaggatgcaa tcaaggtatt
    27241 gagttagcta ctggtgaaaa catattgttg ttaaataatg atgtaatagt tactccaaat
    27301 tggctctata atttgaataa agctttatgg tctcaagata atataggtgc tgtaggacct
    27361 attacgaatg catgttcata ttatcagcaa attgatgttg attataagtc aattgaggac
    27421 atgtttatat ttgcacacaa aataaataaa tctaatatta acctatggaa atatagattg
    27481 aagttagttg gattttgcat gttaataaaa aaaactgttc tggataatgt aggggtttta
    27541 gatgaattat tcacacctgg aaattttgaa gatgatgatt tatcttttag gatgatagag
    27601 tcaggatata agttattatt atgccaggat acttttatac accattttgg aagtgtgtct
    27661 tttaaagaaa atcgtaataa atatacagat ataatgtata ttaatagaag aaaatttgaa
    27721 actaagtggg gatttaatcc agaatatagt ggaatgataa gaacagattt gatttctaaa
    27781 atagatgata gtttggaggc aaatattaat gttttagaaa taggatgcgg tataggggct
    27841 actcttttag aaataaaaaa tatatataaa aatgcatcaa tatatggaat agaaattagt
    27901 gagtcagcag gtaaacttgc aaagtatagt gcagatgtac ttatagcaaa tatagaaaaa
    27961 gtaaaattgg attataaaaa agattttttt gattatataa tattgggaga tgtacttgaa
    28021 catttgattg acccatggaa gatagtcaat gaattgaaac aatatctaaa gaaggatggt
    28081 tatctggtat ctagtatacc aaatattatg catgtttctg ttttaagaaa tcttgtgtta
    28141 ggtaatttta catatgaaga ttcaggaatt ttggacaaga ctcatttaag gttttttaca
    28201 ttaaatgaga taacaaaaat gtttaatgat agtaattaca taattaatag tattacagct
    28261 actagtatgc cactttcgga aattgataat agcttcattg atacaatatg taaggaaagc
    28321 tgtaaagaaa tgagacaaca gtacgaatct tatcaatata tagtaaaagc ctctaaaatc
    28381 ctagatacta aaagatattc tagtattagt atgattaatt taaaatacgc tttaatgaga
    28441 attgataatg aattagatgt agatagtaat ttaagttata tatttgataa ttataatata
    28501 actgaaccat gttttgttga tgatattgaa tatctaattg atacatttgt gataaataaa
    28561 gcaactctat taaatagatt aggagttgag gcatttaaca gaggtctagg agattttagt
    28621 ataaacatgt ttatgacagt attagagatg gatagagatg atattgactc aatatataat
    28681 ataacaagtg tattatgtga attagaagaa tttgatactg cttataattt aattaaaaat
    28741 agtagcgaaa aagtacagag tgaaagtgat atacaagaga tactttgttt tattgagggg
    28801 aagaaatatg aataatgata agatatgttt tataacctgt gtaaatgatg aaaggcttta
    28861 tgaagaatgt cttgtatata taaatagcct agacattcct aaaaacttta gtatagaaac
    28921 agtagcttta agaggagccg attctattgc ctctgcttat aataaagcta tgagagaaac
    28981 agattctaag tataaggtat acttacatca agatactttt ataattaata aaaatttcat
    29041 acatgatatt ttagagatat ttaaaaaaaa tacagatgtc ggaatgattg gtgtagctgg
    29101 agtaaaagat atgcctgtga atggaatttg gtgggattct gataaaaaag cagggaaagt
    29161 atatgatagt catacaggta aaatggatat ttatgaattt agtaactttg aagaatgttt
    29221 taatgaagtt atggctatag atggattaat tatgattact caatatgatg tattttggag
    29281 agaggataag tttgatgggt ggcattttta tgatttgtca cagagtatgg agtttagaca
    29341 aaataaatac aaagttattg taccaaaaca aaataagcct tggtgtatgc atgattctgg
    29401 agtagtaaat ataaataatg gatatgatga atataggata aaatttgtaa aagagtacat
    29461 aaatcttgat ttatatagag aaaattttta tgaatttgat aagaagaaag caagtaccat
    29521 acttaagagt aataagcatg taatggattg tatgaaagag gagcttacag aacaattaag
    29581 attaaagaat tacaaggatg ttttaggtat tgtttctaaa attgctcatt tttctgtttt
    29641 taatcatact ggtttgtatt cttgtgaaga atgtgagaga gcattgctag aatgtgcaaa
    29701 taatttacca gaaatagaaa atacgaaatt atttacaaaa aaagatttaa aaagaagaaa
    29761 agtattacat gttttaagtg aaggatattc aacaggaggt catacaagac tagttaaaaa
    29821 ttggattgaa attgacaaag actctgttca ttcagttgta actacatggc aatcagatac
    29881 actgccagac tggttattaa aagcaataaa tgatagtggt ggcgaagtta tatctttgga
    29941 tttaatttca gatgattatt taaaaagagc tgtagctttg aggaaaatag cttatgactg
    30001 ggctgatata gttattttat atgttcatat gtgggataca atacctgttg ttgcttttgg
    30061 agttgatggg aaaataccta ttttacatgt taatcactat gatagtactt tttggttagg
    30121 gacaagtatt gcagatattg tcattgattt tagcgaaact agtcaattaa ttacattaga
    30181 taaaagaggc atagataaat ctgaaatatt accaatgcca ttaaaagaaa aagaagtatt
    30241 ttcaaaatct gaagttagaa aacgatataa tttagaggat gatgtgacaa taatattgac
    30301 tattgctagt gaaagaaaat acaatagtgt aaatgatatt aattacttta aaattgctaa
    30361 aacattgatt gaaaatacag ataatacaat tattattgtt gtaggagttg atacagagaa
    30421 tctctattgg aaaactttaa gtaatgttac agataataga gttatatgtt ttggaacaac
    30481 agcaaatata gatgattttt ataagatttc ggatatatac ctagagtctt tcatggtagg
    30541 aagtcatact tcaaaattag atgcaatttt atatgattta attcctgtta agtttaaaaa
    30601 tgaagtttca ccaattttta caaatatgtg gaattttata gataagtttg attataagaa
    30661 tataaaagag gttatagaca tgataagaag gtacaatact agtgaatttg acagggataa
    30721 gataaaagca aaacaatcgg aaataaaaca ttttgttata gataaacact gtattgaaat
    30781 aaaaaactat atagacaaaa tatataatga agttaatcat cataaagtta agtatgattt
    30841 atataaaaat gatgaaatcg aggactatca gctttttgta gcattatatg gctatatggt
    30901 aaaaaataaa aacatgtcat aggtggtaaa atgaacaata ttcatatcaa taaatttgaa
    30961 aaaagctgtg aaaaaaactg ggattatttt gtagaaaata aatctataaa tggtacattt
    31021 ctgcaaacta gaaatttcct taattatcat gaggagggaa aattcactga ccactcatta
    31081 ataatatgta aaggtgaaga aatactagca gtcgtacctg catgtgaaac ttatgaagat
    31141 ggtgaaagaa tatttttttc tcatttaggg agtacttttg gtggcattat aataagaagt
    31201 ggttttaatg atattaggca tgtaaatgcc attacaaata agttagaaat atatttacag
    31261 gaacaaaatt tcgataaagt atatttaaaa aatacatcaa gaatattttg taaagaggat
    31321 acttcattac tagaatattt tttatttaag aataattaca tgttttatga tgaattaagt
    31381 atgtttttag aattaagtaa gtataaagaa gatatattag ccaattttaa atacagtaaa
    31441 agaagagatt ttaaatattc tttgaagaat aatcttacct ttaaaagact gtatagtgat
    31501 gaagatatag tttgtttttt taatttactt tctaattgtt tagatagtaa aggagctaaa
    31561 gtaatacatt cagtagaaga acttttggag tttaaaaata atagattaaa agatattgta
    31621 gaattttatg cagtatatat tgatgacatt atgatttgtg ggagcatggt atttaacttt
    31681 caagaaagag tttatcatac tcaatatcta gcatcaaatt cgacatataa taaatattat
    31741 ccaatgatat ttttatacta taatctaata aaagatgctt tagagagaaa ttttaagtat
    31801 ctttcttttg gaattagcac tctaaataga ggcaaatact tagatgaggg tttagctaca
    31861 tttaaagaag gttttggttc aagtgcaatt tgtaacagaa catactataa aaatataaat
    31921 aagtgagaat catgttttat agagttttta gagtatagta aatatctagt actataatat
    31981 tacataaaaa taattagttt ataaagaagg tttgtatatg aaaagattaa tgattttagg
    32041 ctcaattaag tattttgaga atattgttat gtcagctaaa agagagggaa tatacacaat
    32101 tgtttgtgat aatagaattg atactcctgc gaaaaagatt tgtgatgaag caatagatgt
    32161 tgatgtattt gattttgata aaataaagaa tatagctata gataaaaaga tagatggaat
    32221 tttaactgga tttacagatt ctttaatgaa gccatatgtt tatattgcaa atgaactaaa
    32281 tttgccatgt gtgatttctt gcaatcaact tgaatctgta actaataagg ctgttatgaa
    32341 aaattatttt aaaaataatg gaatacctac aactgattat tgtattattg aaagtttgaa
    32401 agatttagaa aaaatacaag gattaaattt tccacttgta ataaaacctg ttgatggtta
    32461 tggttcaaga ggtgtatatt ttgtatcaaa catagaagaa ttaatagaaa aatttgaata
    32521 ttctaaggaa gcctcgcaag atggacaagt aataattgag gaattttatg aaagtgagga
    32581 aatacaagga ctagcttggg ttcataatgg agagtcacat gtattttata ttggagatag
    32641 agagcttgta aatatacata caggaagagc tggaaaacca gatagattaa tttatccatc
    32701 aaagtattgt tttcaatatg aagaagaaat aaaatccata tatcaaaata taacgcaggc
    32761 ttttggcata aaaaatggac ctctttatgt acaaatgtta gtgggagcat atggaataaa
    32821 ggtatctgaa gtaatgccaa gattacctgg aggttgtgac tatatggcta tttctcaaat
    32881 aacagattta gacataggaa gtttatttgc aaactttagt gtgaataatg aagtaaattt
    32941 tgatgaaata aagaaatata atatgcatct atcaaaatgt gtatatgcat taccaatata
    33001 cataagacct ggcacaataa aaaagataaa taatatagat aaaatagaaa acttagatta
    33061 tgtcactcaa tttctattac tagtaaaaga aggagataca ataggattat atgaagatga
    33121 gagacaggat tgtggaagag tttatggagt agcaaataat gtatttgatg caaatataaa
    33181 gaagaaatgt attcatgaaa tgatagaaat acttgatgaa aataatgaaa atatgataga
    33241 aaatttttaa ataagggagt atgatagata tgaagctaat aactcatgaa caaattatgt
    33301 cattaaacat ttctcagaaa caaggatacg agtggataaa agaatccttt ttaacaaaaa
    33361 aagattgtca attaccacca aaaataagca tggtagaaga agggcatgta ttttacaatg
    33421 taatgccgtc cctaaatcat aatcataaga ttggtggaat aaaaatggtt acaagattgc
    33481 cagggagaaa tccaagttta aattcagaga tatttctata taatatggaa gatgctaaat
    33541 taaaggctat tttagatggc aattatatta cagcttttag gactgctgct gttgctgcct
    33601 tggctatcga tacatttgct aaaaaagatt ttaatactat tgctattatg ggtttaggtg
    33661 taactgcttc tgctataatg aatatgcttt catatactct taaagataaa aatataaata
    33721 taaagttact taaatataaa gaccaagcag agtcgtttgt aaaaaagtat gaaaaaaatg
    33781 aacaatttag ttttgaaata tgtgacacac atgaagaact tattgtagat tcagatgtag
    33841 ttatttctac tgtgacatat acagatgaat atttatcaga atttaaatgg tttaaaaaag
    33901 gtgctttatt attacctgta catgttatgg gatttggaaa tgttgattat ctatttgata
    33961 aagtatattt ggatgattat aatcatttga aacatctaga aaatatagat agatataaat
    34021 attcttgtga attttatgat gttttaagta aacaagctaa aggaagagaa aatgatgatg
    34081 agagaataat atcttacaat ataggtttgt caatacatga tatattattt gcaaataaaa
    34141 tttacgaatt gtgtgaaaca ctaagctgtg aagaaataga attaaaagaa ccaaaagaaa
    34201 aatattggat atagtttatt tcattcatat ttacaatagt ttattgtaaa tatgaatatg
    34261 aagtaatata ttgttagaaa attagcatga ataggataga taaatttatg gataaatata
    34321 atttaattag gttcgtatct attggaaacg aatatatagg aaatttagtt gcattagaat
    34381 caaataaagc aatacctttt gatataaaga gagtttatta catatatgat gtaccaaaca
    34441 atgtaaagag aggatttcat gcacacaaaa acttggaaca agtactagtt tgcctaaatg
    34501 gaagtgtgaa gataaaatgt tatgatggtg taaaagagac tatatttaat ttacataaaa
    34561 atgatgttgg attatatata ggaacgggag tgtggcatga gatgtttgat tttgatgaaa
    34621 aaactgtttt aatttctata gcatctgaac attataatga agaagattat attagagact
    34681 ataaagaatt tttaaagtat ttaaataatg tgtagttatt atacaattag tgatgtattt
    34741 tcttaattaa ataaatttaa gatagccaca tagagaatag atagattagg atgtgaattt
    34801 attgttggtt aaatttaata atttattaaa acagttccaa actcataaag aagaatatga
    34861 acagaagatt aaggatgtaa tggaaagtgg atattatatc cttggaaatg aagtaaagca
    34921 ttttgaagaa gaatttgcta actatgtagg tagcaaatat tgtgttggtt tagccagtgg
    34981 tttagatgca ctgataattg cttttagagc attaggaata agcaaaggtg atgaagtaat
    35041 cgtgcaatca aatacatata ttgctagtgt tatgggaatc agtataaatg gagcaacacc
    35101 agtatttgta gaaccaaatg aatattataa tatagattca tctaagatag aagaaaagat
    35161 aaatagcaaa acaaaagcta tattagtagt gcatttatat ggtcaagcaa gtaatatgaa
    35221 agatatacaa gaattatgtg ataagtataa cttgtattta attgaagatt gtgctcaatc
    35281 tcatggagct aaatatgatg gaaaaatgac aggaacattt ggtgatatag gttgttttag
    35341 tttttatcca agtaaaaatt tgggtgcatt tggagatgca ggagctatag taactagtaa
    35401 tgaggaaata gctgataaag tgaaaattct tagaaactat ggctcagaaa agcgatatta
    35461 taataaattt gtcggatata attcaagatt ggacgaaatt caggcaggtt tattaagagt
    35521 aaaacttaaa tatctaaatg aaattgaaat tgaaaaatca aaaatcgtag atagatatct
    35581 taatgaaatt aataatccca aagtcatact tccaaagata gaaaataatt gtactcatgt
    35641 atggcattta tttgttttac aagttgaaaa acgagataca tttatggaat acttaaagaa
    35701 aagtggtata gagtctataa tacattatcc gataccacca catctatctg aagcatatgc
    35761 ttatttggga tataaaaagg ggagttttaa tatagctgag aactattcag ataaagttgt
    35821 tagtatacct ttatttattg gtatggaaga tgaagaagtt agttatataa ttaaaattat
    35881 aaatgaatat taaactactc ataataatat caaaaataaa ttttttatat atcatttatt
    35941 taaatttcgt taattga
//
LOCUS       DAELLV010000013        35594 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.013, whole genome shotgun sequence.
ACCESSION   DAELLV010000013 DAELLV010000000
VERSION     DAELLV010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35594
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.013"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            157..297
                     /locus_tag="KPD58_000589"
     CDS             157..297
                     /locus_tag="KPD58_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264376.1"
                     /translation="MGIIESASKLAEMVHLLAVEKGITDIEAWDEAVKEYSKIYEERR
                     NE"
     gene            complement(472..792)
                     /locus_tag="KPD58_000590"
     CDS             complement(472..792)
                     /locus_tag="KPD58_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBG0264377.1"
                     /translation="MKLKTISLPELNNLDPTLESTFIKMGEEQGELAECIGKFRNLSG
                     ENNDLDEVDIIKKTAKELMDVAQTCVTMMFKLEEQYGINLDEIRKEHIKKLEKRGYIK
                     NMDK"
     gene            complement(912..1649)
                     /locus_tag="KPD58_000591"
     CDS             complement(912..1649)
                     /locus_tag="KPD58_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBG0264378.1"
                     /translation="MDANSLKDLIANHNNIVFFGGAGVSTESNIPDFRSSTGLFSQKL
                     NKQFTAEQLVSHTFFVRYPEEFFEFYKDKLIYPNAKPNNAHIALAKLEEMGKLKAVIT
                     QNIDGLHQMAGSKNVLELHGSVHRNYCTKCGKFFDLESMLNLGGNIPYCDNCGSIVKP
                     DVVLYEEALDSDVITKTISAISNADLLIIGGTSLAVYPAASFIDYYKGDYIALINKAN
                     TVYDKSASLVINKPIGEVLYEAVLRQI"
     gene            1864..3687
                     /gene="acd"
                     /locus_tag="KPD58_000592"
     CDS             1864..3687
                     /gene="acd"
                     /locus_tag="KPD58_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HBG0264379.1"
                     /translation="MKKKAALATLAMLPLGVVNAHADGDIGIVTINYLNVRNEPTAES
                     SIAFVAKKDDKVLIKDSSNGWYKIKAESGQEGWASSKYIAKSNSDSLRTSTNKEKQVI
                     SNSLNMRNGAGTSYRVITVLKKGQKVEVISESNGWSKIKYDGRLGYVSSSYLGDVSNS
                     TNKSKTKQVNTTSLNVRSGPNTSYGLLGKLPKGSKVEVISESNGWSKIKYNGKDAYVS
                     SMYLSDVSQSNSDNSSQSNDKKNTDKVVNTASLNVRSGPGSTYSKLGKVYKGSKVTVL
                     SESSGWAKINFNNKEAFVVGNYLSTSADTSNNNSNSNSDNSSNSNGNNSSSSGQVNGM
                     SGISGAKIDYKSLSYTLESHISKQVEKAASGGNVIAPSNRKSTPSPEFSTFSAQRTSS
                     FVNASSSDIEYYLNPKNFTNTTKGMMQFLKINSYRDGISESSLNSYLNGLSSSVFKNQ
                     GAAFINAAKKYNIDVVYLVSHAMWETAYGKSTLAQGQTLTSYKGQPLSKPVKVYNFFG
                     IGAIDKSANVSGAEAAYSNGWTSVEATIDGSAKWISQNYVNSSKYNQNTIYKMKWNYD
                     YTWHQYATDVNWANGISGIMENLIGLYGGGSSLVFEVPQYK"
     gene            3902..8200
                     /locus_tag="KPD58_000593"
     CDS             3902..8200
                     /locus_tag="KPD58_000593"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HBG0264380.1"
                     /translation="MESIKEYLDKLEINNSGLGKQLKEVYINRVVYFKEDKIVYFYLT
                     SKDIVSHELLDKFKEELMYKLDYFKDMRMKIRFTGLERKSNKDVIKKYWNNILYILKY
                     LCPSIAGWYKQVEFLCLEEELKIKLPKGIFYERLMKKNVTYVLKTVLSEELGLDLNIT
                     IEKAVDEKVNKERLIRINDREMEEKIRALEIGKVNNCEENEEESYVIKSDVDENLIYG
                     DNANAMIENIVELNASSGTVAVVGDIFDVDTKELKNGKILMIASITDYTSSISCKLFL
                     TDTNKDGVLENVKKGAYLKIKGDIVYDTYQREISMMISGIRKETRIEREDKSEEKRVE
                     LHAHTQMSSMDAICSVKKLVERAAKWGHSAIAITDHGVVQGFPDAMNAGKSNNIKILY
                     GVEGYLVEDDSLIIEDANDKELSQTFVVFDIETTGFSNTNDKITEIGAVKIENFKIVD
                     RFSELINPEKDISYKIQELTGITNELIKDKPTIEEVLPKFMEFVGDSVLVAHNAEFDT
                     GFISQKCREQGLLYNNKKVDTLMLARVMLPNLKRHRLNVVAKELGIPLLNHHRAVDDA
                     EATALIFNKFLQMLTEKGAKTLSDVNNILGKIDYTKLSTNHITLIAKNSIGIKNLYKI
                     ISDAHVNHFFRAPRILRSVLNEYKEGLIIGSACEAGVVFQAVKKNVSDEEMKKIIDLY
                     DYIEVMPIDNNRFMIDKGEVKDEEELRELNRKLIDTAKKFDKIPVATGDVHFLDKHEA
                     VLRKVLKYSQGFKVDEEETYLHFRTTDEMLEEFSYLGEELAYEVVVENSNLIADMVED
                     IKPIPDDTYPPIIEGSDVELREMCYKKAKRIYGDPIPEIVQKRLDRELNSIISNGYAV
                     MYIIAQKLVAKSLSDGYLVGSRGSVGSSFAATMSDITEVNPLPAHYICENEDCKYSFF
                     YEIGEWGSGVDLPDKDCPKCGRKLAKNGHDIPFEVFLGFEGDKEPDIDLNFSGNYQPT
                     IHKYTEELFGEGYVYRAGTIGTVAEKTAFGYARKYVEENNISVPNAEVLRLSNGCTGV
                     KRTSGQHPGGVMVIPDYKDVYDFTPIQYPANDTSCGVITTHFDYHSISGRILKLDILG
                     HDGPTIIRMLEDMTGINITEIPLDDKETMSLFTSTEALGVTPEEINCPIGCLAVPEFG
                     TKFVRQMLLDTKPTTFAELVRISGLSHGTDVWVNNAQDLVREDIVGLKDVISTRDDIM
                     NYLIFKGLPPKMSFTIMESVRKGKGLKPEHIEEMKKNNVPEWYIGSCKKIKYMFPKAH
                     AVAYVMTSFRIAYCKVHYPEAFYATYFTTKVEDFDADLIVKGLDAIKSKINEIESLGN
                     DATTKEKGMLTVLEVALEMYARGIKLLPVDIYKSDATEFIVVGEKTLLPPMAAIQGLG
                     GNAAINIQNERKNGEFISKEDLRKRTKISKTVIETLTNHGSLENMSERNQLSLF"
     gene            8404..8865
                     /locus_tag="KPD58_000594"
     CDS             8404..8865
                     /locus_tag="KPD58_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBG0264381.1"
                     /translation="MKKNLEATIEEIVTKITDEHGFEMVDVEYVKEAGEYYLRVYIDK
                     EEGISLNECELVSRELSPILDEKDPIKENYFLEVSSPGLDRALKKDRDFVRYQGRDVD
                     LKLYKPLNGCKQFEGELVGLTEDNNIKIIANGKEMEFNRKDVAIVRLAIKF"
     gene            8895..10049
                     /gene="nusA"
                     /locus_tag="KPD58_000595"
     CDS             8895..10049
                     /gene="nusA"
                     /locus_tag="KPD58_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBG0264382.1"
                     /translation="MNHEFMEALDELVVDRGIDKEILIDTIEQALLTAYKKNFGSAQN
                     VRVEFDRDKGDIKVFSQRVVVDESDLYDTFLEIELADAREINPNYELGDIIEHEVTPK
                     NFGRIAAQTAKQVVVQRIREAEREIVYNEFIEKENEIVTGEVARANKNVVYVNLGRIE
                     GIMTQSEQIPFENYKAGQKIKFYILEVKKTNKGPQIVVSRSHPGLVKRLFEEEVPEIY
                     EGIVQIKSVAREAGSRTKMAVRSIDEKIDPIGACVGPKGLRVKNIVDELGDEKIDIIK
                     YSEDPAEFISAALSPSKVVRVDIDEDEKSALVIVPDYQLSLAIGKEGQNARLAAKLTN
                     WKIDIKSESQAEQEMLNLENSKEIEAEDVEAEFIDQEELDTSEDNFSEEE"
     gene            10056..10337
                     /locus_tag="KPD58_000596"
     CDS             10056..10337
                     /locus_tag="KPD58_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HBG0264383.1"
                     /translation="MILKKVKKVPQRKCIACQDRDSKKELIRIVKNKEGKIFLDPTGR
                     ANGRGAYVCKSSECLKKAIKSKALNKAFKIDVPDEVYDNLLMELEEYEK"
     gene            10327..10638
                     /locus_tag="KPD58_000597"
     CDS             10327..10638
                     /locus_tag="KPD58_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HBG0264384.1"
                     /translation="MKNNKEKVLSFLGLATRAGKIVSGDDSTLLDLKKGKVNLILIAE
                     DASNNTKKLFKDKSTFRNIPYLFFSTKEEIGFAIGKSPRAVVGIKDENFSKKIIELIE
                     I"
     gene            10660..12600
                     /gene="infB"
                     /locus_tag="KPD58_000598"
     CDS             10660..12600
                     /gene="infB"
                     /locus_tag="KPD58_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBG0264385.1"
                     /translation="MSKTRVYQIAEELNISNEELINKLAELDINVTDKDSVLEGEELE
                     LALEMLGEDLSQENGNVIEIDGKLTVQVLATKLDKSPSEIIMKLMKMGTMATINQEIS
                     FEIAALAAKDYGFELTVAESDDTEALEIEALMEIEEDKEEDLKPRPPVVTVMGHVDHG
                     KTSLLDAIRKTDVISGEAGGITQHIGASEVKINGHKIVFLDTPGHEAFTSMRARGAQV
                     TDIAILVVAADDGIMPQTVEAINHAKAAGVPLIVAINKIDKPGANPDKVKQELADQGL
                     LVEDWGGEVIAVPVSAKKKEGIDTLLEMVLLVAEMEELRANPNKRAVGTVIEAELDKG
                     RGPVATVLVQGGTLTVGDPIVAGVACGKVRAMINAKGKRVKTAGPSTAVEILGLSEVP
                     QGGDQFVEVPTDKIARSVAARRQQIVRDEMLKSTQRLSLDALFSQMSEGSIKDLNIVI
                     KADVQGSVQAVKQSLEKLSNEEVQVKVIHGGVGAVTESDILLAAASNAIIIGFNVRPV
                     PGAESLGEKENVDIRTYTIIYKAIEDIQAAMTGMLDPEYVDEETGKAEIREIYKISGV
                     GTVAGCYVTNGKIFRNCKVRLVRDSIIIHEGELAALKRFKDDVKEVNSGYECGMSFVN
                     YNDIKEGDIVEAYITKEVERKL"
     gene            12643..13023
                     /gene="rbfA"
                     /locus_tag="KPD58_000599"
     CDS             12643..13023
                     /gene="rbfA"
                     /locus_tag="KPD58_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBG0264386.1"
                     /translation="MASYNRTRRIAEEIRKVVSTMLINGVKDPRITSMVSVTDVEVTN
                     DLRYAYVYVSILGGDEESTLTGLKSAGGYIRREVGKNIKLRYIPEIVFKLDDSIEKGM
                     YMDSLIKRVNEKNAQQNEDENYDE"
     gene            13004..13987
                     /locus_tag="KPD58_000600"
     CDS             13004..13987
                     /locus_tag="KPD58_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HBG0264387.1"
                     /translation="MKIMMNNMDSIINYIYEGDYFIVTSHISPDGDNIGSTLSMYYTL
                     KNLGKNVYYVLDDEAPLNLRFLVEDVKIYKSNEFNMKNYSLIALDCGDKMRICVSDEI
                     KNNASKLICIDHHASNDSYGDLNYVDIDASSTCELVYNLLVRFQQIKDINIINEDIAT
                     CLYTGLVTDTGNFSYSNVHASSFEMAKNLLVLGAQKNTIIQNIYQSNSSGYYKLLGEA
                     LKGLEIFDSKVSSIVLTQDMMNRNNISFNDVDGITTYTRDIKGIEVGILFKEKKQNEI
                     KVSFRSKNYVDVSEIAKLFGGGGHVRAAGCTIRDSIDNAKKMVLEAVLKSI"
     gene            14000..14905
                     /gene="truB"
                     /locus_tag="KPD58_000601"
     CDS             14000..14905
                     /gene="truB"
                     /locus_tag="KPD58_000601"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBG0264388.1"
                     /translation="MNKIISILKPTGMTSHDVVSRVRKILNIKKVGHTGTLDPDASGV
                     LPICIGKATKVCEVILNKDKSYICELTLGISTDTYDASGEILKKVDDFKFSNEDIERA
                     FDTQRGEINQLPPIYSALKVNGKRMCDLVRSGRQSEITLKTRRVNIKDIKILSIKGNK
                     VMFYVECSKGTYVRSICHDIGEYLGCGAHMSFLNRTSSGKFDLDNSITLEELELFYEN
                     KTLDKYLYDIDYVLDSFNYVVLNPNAIKYYSNGGSIDDKRFLKNNFDKDDEFVRVYST
                     DNFLGLGKLSKHNNTISVKSDKMFI"
     gene            15036..15965
                     /locus_tag="KPD58_000602"
     CDS             15036..15965
                     /locus_tag="KPD58_000602"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HBG0264389.1"
                     /translation="MVIIKSIKEIANIKESVITIGNFDGVHKGHQVLIGKTVEHAKKE
                     NIQSIVFTFANHPVNYFRPNSTKNIISNDDKIKLLEDLGIDIVVIIPFDEYMTKIPAK
                     DFVDEILVKKLKAKKIVIGHDFTFARAKEGNVNLLKSLEHEFGFEVEVIKPIKINDVR
                     VSSTYIRSLVSQGDMANVKEYLGRNYKLEGCVIHSKHLGRTIGFPTANIDLKNNMLVP
                     KRGIYASIVHIGDETYFGATNVGYNPTVNGKTLSIETNILEFDRDIYGENIIVEFLER
                     IRDERKFNSIDDLKNQLYNDTNYVYENYVCKNK"
     gene            16069..16326
                     /gene="rpsO"
                     /locus_tag="KPD58_000603"
     CDS             16069..16326
                     /gene="rpsO"
                     /locus_tag="KPD58_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBG0264390.1"
                     /translation="MINKSEIIKEYGRAEGDTGSPEVQIALLTARINELNGHLKTHKK
                     DHHSRRGLLKMVGRRRNLLAYLKEKDLEGYRALIAKLGLRK"
     gene            16566..17411
                     /locus_tag="KPD58_000604"
     CDS             16566..17411
                     /locus_tag="KPD58_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBG0264391.1"
                     /translation="MKIKLVCDSLCDIPDEISEKDYIEIVPLTVIFNDREYIEGVDIK
                     KEEFYKKVKEIKQIPKTSQATYMEFKEVFDKFITEGYHIICMTGAANASGTFQSAMIA
                     KNDVNENEKIHIFDTRNLSLGSGQYVIKACELLEEGLGFEEIIDELENTRSSVRLLFA
                     PYTLDFLKQSGRVPVATALIGNMLNIKPIFFFDNGEAKLVNKVRGIKNIASKLVDIIL
                     EMNEGSLEGKIVTIGCGDNLHDCEILKDEVNKRIKARRVLFTRGGVSICSHTGPDILA
                     ISCSN"
     gene            17536..19647
                     /gene="pnp"
                     /locus_tag="KPD58_000605"
     CDS             17536..19647
                     /gene="pnp"
                     /locus_tag="KPD58_000605"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBG0264392.1"
                     /translation="MFEHKIFKMDFAGRELSVEIGKICEMASGSCIVRYSDSMVMVNT
                     TKSAKPRDGIDFFPLSVDYEEKLYSVGKIPGGFLKREGKPSEKAILTSRLIDRPIRPL
                     FPKGFRNDVQVVATVLSVDQDCTPDIVAMIGSSIALSISDIPFNGPTGSVCVGLVDGA
                     FVVNPNAEQREKSSMHLVVSGTKEAIMMVEAGADEVPDEVMLDAILFAHQEIKKIVEF
                     IEGIVAEVGKEKMPVELYHAGEEITQLVREFATDKMKKAVQTFEKLERMENMDRVKEE
                     TLAHFEETLEDFEDFVGDIEEVLQDIIKEEVRKLIVHENVRPDNRKLEEIRPIWCETG
                     MIPRAHGSAIFTRGQTQVLNVATLGALGDVQKLDGLDEEENKRYMHHYNFPAYSVGEA
                     RPSRGPGRREIGHGALAERALLPVIPSQEEFPYAIRLVSEVLSSNGSTSQASVCGSTL
                     SLLDAGVPIKDMVAGIAMGLIKHDGKVAVLSDIQGMEDHLGDMDFKVAGTEYGITAIQ
                     MDIKIDGIDKEILQRALKQAKEGRIHILGEMRKTISQPKPELSPYAPKIVKMQINPDK
                     IKDVIGPGGKIITKIIDETGVKIDIEQTGEVFISGIEIDMIKKAQELINNIVVEPEVG
                     KTYKGKVSRIMNFGAFVEILPGKEGLLHISHIAHERVAKVEDVLNIGDEVEVKVTEID
                     EKGRVNLSRKVLLPKPEHKNK"
     gene            19913..20656
                     /locus_tag="KPD58_000606"
     CDS             19913..20656
                     /locus_tag="KPD58_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG0264393.1"
                     /translation="MIMMVLNKSKLKKIIILILVVLLVLSGGVMVLKLFNNKPVFNLF
                     NGVDLPYERGTKDKSGYVALTCNIDLGWETEYVESILETLKKENVKITFNVTGKWAEK
                     NKDELLKIKKQGHEIGNHGYKHLDYSTLSYEDNYEQIETSKKIIEEIIGEKTKFFQAP
                     AGSFGPETVKAAKALGYTSIKWDADTIDWKYKDQPEVIIDRMKKKDIKDSSIILMHPT
                     NATTKCIDDIIAIVREKGLKPGKLSDVFK"
     gene            20814..22061
                     /locus_tag="KPD58_000607"
     CDS             20814..22061
                     /locus_tag="KPD58_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBG0264394.1"
                     /translation="MYKTKILENGLTIIGEEIPYLKSITLGIWINAGSRIEEAQVSGT
                     SHFIEHMMFKGTKNRTSKEIASSIDNLGGQINAFTSKECTCYYVKLIDEHIDTGIDVL
                     SDMILNSKFDKNDIDKERLIILEELKMYEDSPDDLSYDLLVENIYANDGLGMNIIGTK
                     ESLYNITRESMLEYLNKYYIPNNAVISIAGNFNFDDMVEKIKSKFGHWEKKNLSIDIS
                     EAKFNPCFISKNKDTEQVNLAMCLKGIPFENDEEVYSMAVVNNIFGGSISSRLFQKIR
                     EEKGLVYSIYSSQTLYRKCGELGIFASMSTENLQDVYNLIKKEIENIRENYLTEKEIS
                     ESKEQLKGNYILDLESTSSRMMSTGKSMLLSKKVKTTDEILECINNVNINSIKKVVDK
                     VFNIENIGTCIVGRDVEKILHLD"
     gene            22096..22341
                     /locus_tag="KPD58_000608"
     CDS             22096..22341
                     /locus_tag="KPD58_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HBG0264395.1"
                     /translation="MNLSEIAGKEIVNLVTGERLGVIGECDLILDETTGGILALLIPK
                     EKGFFARRKDKSFLEVPWRNVKKIGNDMIIIEYEGYI"
     gene            22351..23574
                     /gene="dapG"
                     /locus_tag="KPD58_000609"
     CDS             22351..23574
                     /gene="dapG"
                     /locus_tag="KPD58_000609"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBG0264396.1"
                     /translation="MSILVQKFGGTSVESYEKMNEVCKIVKAYKKNDEELQLVLVVSA
                     MGRKGAPYATDTLINLCSAVNDEPSKRELDLIMSCGEIISGTILANLLNAQGIDSVFF
                     TGQQAGIITSDEYSNAKIKYINPKKVKRALDDGKVVVIAGFQGVTDDGEITTLGRGGS
                     DTSAVAIGKALECETVEIYTDVDGIMTADPRVEPNAKVLSFIDYEEVFQMADKGAKVI
                     HPRAVQLAKSGNITLAIKNTMNPTFEGTKIGSLCRHLEDNIEYEQERDFKVAVANKDS
                     VAQVKIKSAEEVFTEVLNEIEKKLITIDMINFFISEKAFVVEDADIKSLKEILDKFEL
                     DYEVNRDCAKVTLICSRIADEMSGIMSKVVRGLSKAGVSLLQTSDSNMTISCLVSEED
                     MHTAVHAIHQQFYLK"
     gene            23687..24421
                     /locus_tag="KPD58_000610"
     CDS             23687..24421
                     /locus_tag="KPD58_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HBG0264397.1"
                     /translation="MKDYREDNERFDKDNCSEESSCEERECIKQFGTNEMPPQPPKDI
                     QCITIIGEIEGHFIGNPQKKATKYEHIIPMLYSIEESNDVKGVLVVLNTVGGDIEAGL
                     AIAELLNSTSKKVVTLVLGGSHSIGVPLATAGDYSFIAPTATMIIHPVRTTGLVIGIN
                     ETFEYFKKMQDRIIQFIIRTSNIKKDVLEKLMHEKDELVSDVGSVLIGKEAVDYGLID
                     EVGGLKEALKKLRELIKESEEEKNND"
     gene            24544..26955
                     /locus_tag="KPD58_000611"
     CDS             24544..26955
                     /locus_tag="KPD58_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBG0264398.1"
                     /translation="MATKKKKKKKVNTLSFNAEYHNLITIFIGVFLLYSLNSNSMGWI
                     PVLMQNLFKGLFGGLSIAIPFIVIITGLLGFFDGNEYIYRLRKTKLYYIIILFIFVFY
                     GLLNAGTLPVDSPLKGNMFDDVMKLGVSGQGSGLIATTIAYYMSKIFGIAGGWLISIF
                     ALILSVMFIFNISIKDLVSNAKSKSKASKDSNLTFKDKIANMKKSAIDMMTDEVDDTT
                     INKKPGFFKGLMSKGLNNDDEEDEYFEEENSDGVDDKTIKIVGFNKAEDEYLEILEGT
                     QSMPELDVLKELQKATNENPVVDTKPEKKVDIAKPNLNIEKTQPMSIVAEPVNEDYSN
                     YKKPSIELLNKVNKKSDENGKKKVLKNASLLEKTLSDFGVEAKINQVTVGPTITRYEI
                     QPSPGVKVSKIVNLTDDIALSLAAKSIRIEAPIPGKSAIGIEVPNEEAQMVGVREVLE
                     SEEFNNFNSPLAMGLGKDVAGKIIIGDIGKMPHLLIAGSTGSGKSVCVNTLISSILYK
                     ANPDEVKLLLIDPKVVELANYNGIPHLLIPVVTDPKKAANALNWAVTEMNRRYKLFAD
                     AQVKDVTSYNEKAEEKLPKIVIIIDELADLMMASANDVEDYICRLAQMARAAGMHLIV
                     ATQRPSVDVITGVIKANIPSRIAFAVSSQTDSRTILDMGGAEKLLGKGDMLFYPLGAA
                     KPVRLQGAFISESESEKVIDFVKSQVKDGIKYEEDIIETISKVNTSKGSDEDEFLSEA
                     IEFVVESGQASASMLQRRFKIGFNRAARLIDSMEERGIIGASEGSKPRKVLISKQDLQ
                     NLEGE"
     gene            26958..28016
                     /gene="mnmH"
                     /locus_tag="KPD58_000612"
     CDS             26958..28016
                     /gene="mnmH"
                     /locus_tag="KPD58_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HBG0264399.1"
                     /translation="MSVIKIEKALKLDKVIFVDVRTEGEYEEDHILNAINMPLFKNNE
                     HNDVGTIYKMQGKHEAIQKGFDYVSYKLKDIYLQAAELALNYDNIVIYCARGGMRSGS
                     IVNLLSSLGVNVYQLEGGYKAYRNFVLEYLRHVMDTKNFVSVHGLTGTGKTDLLHLLD
                     EKGIDVLDLEGIAKNSGSTFGFITFDKKPPSQKNFETKLFESIFFSKENYIFVESESK
                     RVGSVTVPNEIFEAMTREGHHILLECDIENRVDRLCRDYIYDKDEGNILILKECINKF
                     RKRLGNKVVDDYIDLLESGEYKELVKRYLLEYYDPLYMHSVEKYKYSKIINFNDTQKA
                     LEDVIDLYESILEGESEC"
     gene            28010..29344
                     /gene="rimO"
                     /locus_tag="KPD58_000613"
     CDS             28010..29344
                     /gene="rimO"
                     /locus_tag="KPD58_000613"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HBG0264400.1"
                     /translation="MLKIALESLGCSKNLVDAEIMMGILNNKGYKLIGDFEEADVIIV
                     NTCGFIESAKQESIDTIINFAELKKTGNLKLLIVTGCLAQRYSEELKTEIPEIDAIVG
                     TGSYQNIDKILKELSEMHKIVSLNDIEFVFNEDLPRYISTPSYMAYLKIGEGCSNNCT
                     YCIIPKLRGKYRSRKFEDIIKEAKKLAESGVKELVVIAQDTTKYGFDLYGKERLSELL
                     EELAKIDGFKWIRVMYSYPESITEELIQVIKKYDNICSYFDMPIQHASNNILKLMNRK
                     TTKEDILNKINLIRSNIPDAILRTTIIVGFPGETEDDFKQLVDFVEEVKFDRLGAFAY
                     SREEDTPADRLPNHIDEEVKIQRRDTLMMIQQKISEELNDKKIGKTYEVLIEEQIEDN
                     VYTGRTQGDAEEIDSIVYVKSVDNLEVGEFVSVQINDAMEYDLMGDVLYELA"
     gene            29331..29873
                     /gene="pgsA"
                     /locus_tag="KPD58_000614"
     CDS             29331..29873
                     /gene="pgsA"
                     /locus_tag="KPD58_000614"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBG0264401.1"
                     /translation="MNLPNKLTLFRIFLIPVFVLIMLLNIPNKFLIACIIFIIASITD
                     ALDGKIARKYNLVTDFGKFMDPLADKLLVISALTCMIEDHLVSSWMVIIIVARELTVS
                     ILRAIAAADGKVIAAGNSGKLKTITQMVSIVFLLLGAQFENVLILNIGEILILIATLL
                     TLYSGWEYLYKNKELFMSSK"
     gene            30161..31207
                     /gene="recA"
                     /locus_tag="KPD58_000615"
     CDS             30161..31207
                     /gene="recA"
                     /locus_tag="KPD58_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBG0264402.1"
                     /translation="MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTG
                     AIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSCVASAQKDGGIAAFIDAEHALDP
                     VYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
                     GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKF
                     YSSVRLDVRKIDTIKQGDKVIGSRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLL
                     DIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTTEIDEKVRAFYNLNEEH
                     EESGTSVSKEIVEE"
     gene            31383..32924
                     /gene="rny"
                     /locus_tag="KPD58_000616"
     CDS             31383..32924
                     /gene="rny"
                     /locus_tag="KPD58_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HBG0264403.1"
                     /translation="MDVIDSVVMIVIGAAVGIIAGYFVRKNISEAKIGQAENLAKEII
                     DKAHHDSETVQKEKLLEAKEEIHKWRTEAERENRERRTEVQKYEKRVVQKEEVLDRKL
                     QNLESKEVNLSEKLKVVEKKEEEVEVIKTQQLEKLESISGITSDKAKEIILTNAERDV
                     RREMSIMIKEIESQAKEEADKKSREIIGYAIQKCAADHVAETTVTVVSLPNDEMKGRI
                     IGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARIALEKLIADGRIHPAR
                     IEEMVEKARKEVDNIIKEYGEQAAFETSVHGIHPELIRLLGRLNYRTSYGQNVLKHSI
                     EVAHIAGIMAAEIGADIRLAKRAGLLHDIGKAVDHEMEGTHVEIGMDLLRRYKESKEV
                     IHAMSTHHGDYEPQTVEAVLVTAADAISAARPGARRETLEAYIKRLEKLEEIANSYEG
                     VEKSFAIQAGREIRIMVKPEAINDEEIHLLARDMTKKIEDELEYPGQIKVSIIRETRA
                     IEYAK"
     gene            33238..35574
                     /locus_tag="KPD58_000617"
     CDS             33238..35574
                     /locus_tag="KPD58_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG0264404.1"
                     /translation="MKKNTLLHTNIMVCIIITLGFVATSIIGYQSNTNIFKNDIEHVS
                     TLAAEGIYYQIDKLLSEPINVSLTMANDSLLKNFLDGEKEHLNDEEFIYKLQDYLNVY
                     RNKYSYDSVFLVSTKTNNYYHFNGLDRNLSANNSENQWYYTFLKNDDEYSLNVDNDEA
                     SNNSITVFVNCKIKDDNGATMGIIGVGLKVNSLQMLLKGYNDKFDVVARLIDDKGFVQ
                     LAVDKTGHENINFFENSSSDLSNSKQLILNNKKEQKSFWYSSEKSKSYIVCQYIPSLK
                     WHLVLENDFTLMIKQLHLQFFKGIAVIVLIIIFVLFTITHVLKKYKNQIIKLSVSQKL
                     EYQKLLSKSTEGLYENIFEIDITNNKAGCEDENRYFHEFGLGLNTPYDEALHTIAKNQ
                     IKEEYAQGYLDTFLPENVIKRYNSGINNISYDFLIKKDEESYHWIRINARIFFWTSDE
                     SVRMISYRKNIDEEKNRELLLLERSQRDSLTGLYNKATTEEFIRSFLKNESNQDLEHV
                     FLIFDIDNFKNVNDKYGHVFGDFVISEFALELKSQFREDDIVGRIGGDEFVVLIKDFN
                     NNNYVLIDKLERFCSKINQKKFVEQAEFSIACSIGVAMYPEHGYVYSELYEKADQALY
                     YAKSHGKNSYHIFSEDSIDSISFHIDSRDIKVLLEMATDGVVKFACMENLKLLYFNQK
                     MLEFTGYSAQTVTSIGFDIFSPVLQDDLQMGLDTLREAISKRETFEIVFRFCHTDGHI
                     FPVRMQGKFVSELYQNRYPVFYAIYTDISSINTLLENK"
BASE COUNT        14347 a         3792 c         6732 g        10723 t
ORIGIN      
        1 aataggtaaa atatcctatt gttttgtaga tatatcctaa ttatgtcaaa aaataaaaaa
       61 atttttattt attagagttt ttggaaataa taataacaga tattataatt aattaacagt
      121 ttttgtaagt atttgacaat agagagggtg taacaaatgg gaataataga gtcagcatca
      181 aaattggctg agatggttca tttattggct gttgaaaagg ggataactga tatagaagca
      241 tgggatgaag ctgtgaaaga atacagcaaa atttatgaag aaagaagaaa tgaatagaat
      301 gataaaaaat gaaaacttga tgataaaaaa taactttatt taaagaaatt caatttttat
      361 agaagtatta aaaataatat aaataaaaag atggtatttc aaatgtaagt aaaaaatcat
      421 tttgggatac catccttatt atttatttta ataatatata agtaataaat cttacttatc
      481 catatttttt atgtaacctc ttttttctag cttttttata tgctccttac gtatttcatc
      541 aagattaata ccatattgtt cttctagctt aaacatcata gtaacacatg tctgcgctac
      601 atccattagt tccttagcag ttttctttat aatatcaact tcatctaaat cattattttc
      661 tccacttaga tttctgaatt tacctataca ttcagctaat tcaccttgtt cttcacccat
      721 tttgataaat gtagattcta gtgttgggtc taaattatta agctctggta aagatatagt
      781 ttttagtttc ataaaagctc ctttcaagtt attcgtattt atactaaaac atcataacaa
      841 aaataatact aaaaaaaaac tttaaattca acataaaaac ttacatatat aataaaaatc
      901 gtgaaataaa tctatatttg tcttaataca gcctcatata aaacttctcc aataggttta
      961 tttattacaa gagaagctga cttatcatag actgtatttg ctttattaat aagagcaata
     1021 taatcacctt tgtaataatc tataaaactt gctgctgggt acacagctaa tgatgtacct
     1081 cctataatca ataaatcagc attacttata gcagaaattg ttttagtaat gacatcactg
     1141 tcaagagctt cctcataaag tactacatct ggttttacta tagaaccaca gttgtcacag
     1201 taaggaatat tacctcccag atttaacata gattctaagt caaaaaactt accacattta
     1261 gtacaataat ttctatgtac agaaccatga agttctagca catttttact tccagccatt
     1321 tgatgaagtc catcaatatt ttgagttatg acagctttaa gcttacccat ttcttcgagt
     1381 tttgctaaag ctatatgagc attattgggt tttgcattag gatatattaa tttatcctta
     1441 taaaattcaa aaaattcttc tggatatcta acaaaaaaag tatgtgaaac taattgttca
     1501 gcagtaaatt gtttattgag tttttggcta aataatccag tagaacttct aaagtctgga
     1561 atattagatt cagttgaaac acctgctcca ccaaaaaata caatattatt gtgattagct
     1621 attaagtctt ttaagctatt tgcatccata gttacacctc caaattatta tatataaatt
     1681 gtactacata ttaggtcgtt tatgttttat taatttaaat aaatatatag tttaataagt
     1741 taaataaagg gaatataaat aaaatgtata cagagtataa atagacaata tttactaaaa
     1801 taacaagttg tcgtataatt atattaaaat tgttttagtg tataagaagg gcgggggaag
     1861 tacgtgaaga aaaaagcagc tttagcaaca ttggctatgt tacccttagg tgtggtaaat
     1921 gcacatgctg atggagatat aggtatagtg actataaatt atttaaatgt aagaaatgaa
     1981 ccaactgccg aaagtagcat agcctttgtt gctaaaaaag atgataaagt tttgattaaa
     2041 gactcttcta atggatggta taaaataaaa gctgaatctg gacaagaagg ttgggcttca
     2101 tcaaaatata tagcaaaatc aaatagtgac tctttaagaa cgtctacaaa taaagaaaaa
     2161 caagttattt caaatagttt aaatatgaga aatggagcag gtacaagtta cagagtaata
     2221 actgtactaa aaaaaggtca aaaagtagaa gtaatatcag agagtaatgg atggtctaaa
     2281 atcaaatatg atggaagact aggatatgta tctagctctt atttaggaga cgtttcaaac
     2341 tcaactaata aatcaaaaac taaacaagtg aatacaactt cactaaatgt aaggagtgga
     2401 ccaaatacaa gttatggttt gttaggcaag ttgccaaaag gaagtaaagt agaagtaata
     2461 tcagaaagta atggatggtc aaaaataaag tataatggaa aagatgccta tgtatctagt
     2521 atgtacttat cggatgtaag tcaaagtaat tcagacaatt ctagtcaaag caatgataaa
     2581 aagaatactg ataaggttgt aaatacagct tctttaaatg taagaagtgg accaggttct
     2641 acatatagta agttgggaaa agtttataaa ggaagtaaag taactgtact atcagaaagt
     2701 agtggatggg ctaagattaa ttttaacaat aaagaagcat ttgtagtagg caattattta
     2761 tctacttcag cagatacttc aaataataac tcaaatagta actctgataa cagttctaat
     2821 agtaatggta ataattcttc atcatcaggt caagtaaatg gtatgtcagg tataagtggg
     2881 gctaaaattg attataaatc tttgagttat actttagaat ctcatataag taaacaagtt
     2941 gagaaagcag catcaggagg aaatgtgata gctccaagta ataggaaaag tactccaagt
     3001 cctgaattta gtactttttc agctcaaaga acaagctcat ttgtaaatgc aagttcaagt
     3061 gatatagaat attatttaaa tcctaagaat tttacaaata ctactaaagg tatgatgcag
     3121 tttttaaaga ttaacagtta tagagatggt atttcagaat caagtcttaa ctcgtatctt
     3181 aatggtttat cttctagtgt atttaaaaat cagggagcag cttttataaa tgctgccaag
     3241 aagtataata tagatgttgt atatctagta tctcatgcta tgtgggaaac tgcttatggt
     3301 aagtctacac ttgcccaagg tcaaacatta acatcttata agggacaacc tcttagtaag
     3361 ccagttaaag tatacaattt ttttggtata ggagcaatag ataaaagcgc taatgtttca
     3421 ggtgcagaag cagcgtattc caatggctgg actagtgtag aagctacaat agatggctct
     3481 gcaaagtgga tatcacaaaa ttacgtaaat agttctaagt acaatcagaa tactatctac
     3541 aagatgaagt ggaattatga ttatacatgg catcagtatg caactgatgt aaactgggct
     3601 aatggaattt ctggaattat ggaaaattta attggtcttt atggaggagg aagtagtttg
     3661 gtttttgaag ttcctcaata taagtaatac ataaataaaa aatatagact atgggtagtt
     3721 ttcattttat ttaaattatc tatagtcttt ttgtatataa tttataatct gtaaattcaa
     3781 tgttaacaaa acttttatgt tataatatta attctaagta aatataagtg agtaataggt
     3841 agaaatagat aaaaagtggt aataatatat ataaagataa ttttgagaaa ggaggatata
     3901 tatggaaagt attaaggaat atttggataa gttagaaatt aataatagtg gtcttggaaa
     3961 gcaacttaaa gaagtttata taaatagagt agtttatttt aaggaagata agatagttta
     4021 tttttatcta acttctaaag atattgtttc tcatgaactt ttagataaat ttaaagaaga
     4081 attgatgtat aaactcgatt attttaaaga tatgaggatg aagataagat ttactggtct
     4141 tgaaagaaaa tccaataaag atgttataaa aaaatattgg aataatatat tgtatatatt
     4201 aaagtatctt tgtccatcta ttgcagggtg gtataaacaa gtagaatttc tttgtttaga
     4261 agaagaatta aaaataaaac ttccaaaagg tatattttat gagcgattaa tgaaaaaaaa
     4321 tgtaacttat gtacttaaaa ccgtgcttag cgaagagttg ggattagatt taaatataac
     4381 aatagaaaaa gctgttgatg aaaaggtaaa taaagaaaga ctcataagaa tcaatgatag
     4441 agaaatggaa gaaaaaatta gggctttaga aattggaaaa gttaataact gtgaagaaaa
     4501 tgaagaggaa agctatgtca ttaagtctga tgtagatgaa aatttaatct atggagataa
     4561 tgcaaatgct atgattgaaa atatagttga attaaatgca tcttctggaa cagttgcagt
     4621 tgttggagat atatttgatg ttgacactaa agaacttaaa aacggaaaaa tacttatgat
     4681 agcttcaatt acagattata ctagctcaat aagctgtaaa ctttttttaa cagatacaaa
     4741 taaggatgga gtacttgaaa atgttaaaaa aggagcatac ttaaaaatca agggtgacat
     4801 agtttatgat acatatcaaa gagaaattag tatgatgatt agtgggataa gaaaagaaac
     4861 tagaatagag agagaagata aatcagagga aaaaagagta gaactccatg cacatacaca
     4921 aatgtcttct atggatgcta tttgttctgt taaaaagctt gtagagcgtg cagccaaatg
     4981 gggtcattct gctattgcta taacagacca tggtgttgta caaggatttc cagatgctat
     5041 gaatgcaggt aagtcaaata atataaaaat actttatgga gtagaagggt acttggttga
     5101 agatgattct ctcattatag aggatgcaaa tgataaagaa ctttcacaga catttgttgt
     5161 atttgacata gagacaactg gtttttctaa tacaaatgat aaaataactg agattggtgc
     5221 tgtaaaaata gaaaacttta agattgtaga tagatttagt gagttaataa atcctgaaaa
     5281 agatatttct tataagatac aggaattgac aggaataact aatgaattaa ttaaagacaa
     5341 gccaaccatt gaagaagttc ttcctaagtt tatggaattt gttggagata gtgtattagt
     5401 agcacataat gcagagtttg atactggatt tatatcgcaa aaatgtaggg aacaaggctt
     5461 gttatataat aacaaaaaag tagatacact tatgttagca agagtaatgt tgccaaactt
     5521 aaaaagacat agattaaatg tcgttgcaaa agaacttggt attcctcttt taaatcacca
     5581 tagagcagtg gatgatgctg aagctacagc acttatattt aataagtttt tgcagatgct
     5641 tacagaaaaa ggtgcaaaaa ctctaagtga tgtaaacaat atacttggaa agattgatta
     5701 cacaaagctt agcacaaatc atataacctt gatagcaaaa aattctatag gtataaaaaa
     5761 tttatataaa ataatttcag atgcacatgt aaatcatttt tttagagcac caagaatcct
     5821 tagaagtgta ttaaatgaat ataaagaagg attaataatt ggttcggctt gtgaagctgg
     5881 ggtggttttt caagctgtaa agaaaaacgt gtctgatgag gaaatgaaaa aaataataga
     5941 tttatacgat tatattgaag taatgcctat agataataat agatttatga ttgataaagg
     6001 agaagtaaag gacgaagaag agttaaggga gttgaatagg aaattaattg atacagccaa
     6061 aaaatttgac aaaattccag tagctactgg ggatgttcat tttttagaca aacatgaagc
     6121 tgtcttaaga aaagttctta aatattctca aggttttaaa gtagatgaag aagaaactta
     6181 tttacatttt agaacaacag atgaaatgtt ggaagagttt agttatcttg gagaagaatt
     6241 ggcatatgaa gttgtagttg aaaatagtaa tctgattgcc gatatggttg aagatataaa
     6301 acctatacca gatgatactt atcctccaat tatagagggc tcagatgtgg agttaagaga
     6361 aatgtgttac aaaaaagcca aacgaatcta tggagaccca ataccagaaa tagttcaaaa
     6421 aagactggat agagaattaa attcaataat aagcaatggg tatgcagtta tgtatattat
     6481 agctcaaaaa ctggttgcaa aatctctttc agatggatat ttagttggtt caagaggctc
     6541 agttggctca tcttttgcag ctactatgag tgatataaca gaagttaatc ctcttccagc
     6601 acattatatt tgtgaaaacg aagactgtaa atattcattc ttttatgaaa taggtgaatg
     6661 gggctcaggt gttgatttac cagacaagga ttgtccaaag tgtggaagaa aacttgctaa
     6721 aaacggtcat gatattccat ttgaggtttt cttaggtttt gaaggtgata aagaaccaga
     6781 tatagactta aatttctcag gaaattatca gccaacaatt cataaataca cagaagaact
     6841 atttggagag gggtatgtat atagggctgg gactataggt actgtagcag aaaaaacagc
     6901 ttttggatac gcaagaaaat atgtggaaga aaataatata agtgtaccaa atgcagaagt
     6961 acttaggctt tcaaatggat gtactggagt aaaaagaacc tcaggacaac atcctggtgg
     7021 agttatggtt ataccagact ataaagatgt atacgatttt acacctatac agtatccagc
     7081 aaatgatact tcttgtggtg ttataactac tcactttgat tatcattcaa taagtggtag
     7141 aatacttaaa cttgatatac ttggacacga cggtcctacc attataagga tgcttgaaga
     7201 tatgactgga ataaatataa cagaaattcc tttagatgat aaagagacta tgtctctatt
     7261 tacatctaca gaagcattag gtgttacacc agaagaaata aactgtccta taggttgcct
     7321 tgctgtacca gagtttggaa ctaagtttgt acgacaaatg ttacttgaca caaaacctac
     7381 tacatttgca gaacttgtac gtatatctgg tctctctcat gggacagatg tttgggtaaa
     7441 taatgcacaa gacttagtta gagaagatat agttggatta aaagatgtta tatctacacg
     7501 agatgacata atgaactatt taatatttaa aggattacct cccaaaatga gctttacaat
     7561 aatggagagt gtaagaaaag gtaaaggttt aaaaccagaa cacattgaag aaatgaaaaa
     7621 aaataatgtt ccagaatggt atataggctc atgtaaaaag attaagtaca tgttcccaaa
     7681 agcacatgct gttgcttatg taatgacatc atttagaatt gcatattgta aagttcacta
     7741 tccagaagca ttttatgcga cttattttac gacaaaagtt gaggattttg atgcagattt
     7801 aattgttaaa ggattagatg ctataaaatc aaaaataaat gaaattgagt cactgggaaa
     7861 tgatgcaacc acaaaagaaa aaggtatgtt gaccgtttta gaagttgctc ttgaaatgta
     7921 tgctagagga ataaaattat taccagttga tatatataaa tcagatgcaa ctgagtttat
     7981 agttgtagga gaaaaaacat tacttccacc aatggcagca atacaaggtc ttggaggaaa
     8041 tgcggctata aacatccaaa atgaaagaaa aaatggtgaa tttatatcaa aagaagattt
     8101 gagaaagaga acaaaaatat caaaaacggt aatagaaact cttactaatc atggttcact
     8161 tgagaatatg agtgagagaa atcaattgtc attgttttaa gttagcccaa aaagttgcaa
     8221 ataaaagaat tttatgatat catagtatag aaaatagtat actttgcaga aactaagagt
     8281 gagacagctg ttctcactct tttattttaa atatattatt aaaaagctgg tttattttta
     8341 ttaaatatat tttatttaag attatataaa ctggtcattt acaattgaat aggaggaact
     8401 ttaatgaaaa aaaatttaga agctacaatt gaggagattg taactaaaat aacggatgaa
     8461 catggatttg aaatggttga tgttgaatat gtaaaagaag caggagaata ctacttaaga
     8521 gtatacatag ataaagaaga aggaatatct ttaaatgaat gtgaattagt tagtagagaa
     8581 ttaagtccaa tactagatga aaaagaccct ataaaagaaa attacttttt agaggtatct
     8641 tctccagggc ttgatagagc attaaaaaaa gacagagatt ttgttagata tcaaggtaga
     8701 gatgtagatt taaaattata taaaccatta aatggatgta agcaatttga aggtgaactt
     8761 gtaggattaa cagaagataa taatataaaa atcattgcaa atggtaagga aatggaattt
     8821 aatagaaaag atgttgctat tgtcagatta gcaataaagt tttagtggta tattaaatag
     8881 ggaggatata aaaaatgaat catgaattta tggaagcatt agatgaatta gtagttgaca
     8941 gaggtataga taaagaaata cttatagata ctatagaaca agctctttta actgcataca
     9001 agaaaaactt tggttcagca caaaatgtta gagttgaatt tgatagagac aagggagata
     9061 taaaagtatt ttctcaaaga gttgttgttg atgaatcaga tttatatgat actttcttag
     9121 aaatagagtt agcagatgct agagagataa accctaatta tgaattaggt gatattatag
     9181 aacatgaagt tacacctaaa aactttggaa gaattgcagc tcaaacagca aagcaagtag
     9241 ttgttcaaag aataagggaa gctgagagag aaatagtgta taatgaattt atagaaaaag
     9301 aaaatgaaat agttacagga gaggttgcta gagcaaataa aaatgttgtt tatgtaaatc
     9361 ttggaagaat agaaggtata atgactcaaa gtgaacaaat accatttgag aattataaag
     9421 caggtcaaaa aataaaattc tatatacttg aagttaaaaa gactaacaaa ggacctcaaa
     9481 tagttgtatc aagaagtcat ccgggtctag taaagagatt gtttgaagaa gaagttcctg
     9541 aaatatatga aggaatagtt caaataaaat ctgtagcaag agaagcaggg tcaagaacta
     9601 aaatggcagt aagatctata gatgaaaaaa tagacccaat tggagcttgt gttggaccaa
     9661 aaggacttag agtaaaaaat atagtggatg aacttggcga tgagaaaata gatataataa
     9721 aatatagtga agacccagca gagtttatat cagcagcact tagcccatca aaggttgtta
     9781 gagtagatat agatgaagat gaaaagtctg ccttagtaat cgttccagat tatcagttat
     9841 cgcttgctat aggtaaagaa ggtcaaaatg caagactagc agcaaaattg actaactgga
     9901 aaatagatat taagagcgaa tctcaagcag aacaagaaat gttaaatctt gaaaattcca
     9961 aagaaataga agctgaggat gttgaagctg aatttatcga ccaagaagag ttagatactt
    10021 cagaggataa ttttagtgaa gaagagtaag gagggatgat tttgaaaaaa gtaaaaaaag
    10081 tgcctcaaag aaaatgcata gcatgtcaag atagagactc taaaaaagag cttataagaa
    10141 tagttaaaaa taaagaagga aaaatatttt tagacccaac aggaagagcc aatggaagag
    10201 gagcatatgt atgtaaaagc tctgaatgtc taaaaaaagc tataaagagt aaagcattaa
    10261 ataaagcttt taaaatagat gttcctgatg aagtctatga taatctactt atggagttag
    10321 aagaatatga aaaataataa agaaaaagta ctttcgttct tagggctcgc aactcgtgca
    10381 gggaaaatag tgtctggtga tgattcaact ttgcttgacc ttaaaaaagg aaaagtaaat
    10441 ttgattttaa tcgcagaaga tgcatcaaat aatactaaaa aactttttaa agataaatca
    10501 acttttagaa atattccata tctatttttt tcaacaaaag aggaaatagg atttgcaata
    10561 ggaaaatctc caagagcagt tgtaggaata aaagatgaaa atttttctaa aaagataatt
    10621 gaactaatag aaatttaaaa taaaataggg ggtgatcttg tgtcaaaaac aagagtatac
    10681 caaatagcag aagaattaaa tatatcaaat gaagagctta taaataagtt agctgaatta
    10741 gatataaatg taactgacaa agattcagta ttagaaggtg aagagttaga attagcactt
    10801 gaaatgttag gtgaagattt atctcaagaa aatggaaatg ttatagaaat agatggaaag
    10861 ttgacagtac aagtattagc aacgaaatta gataaatctc catcagaaat aattatgaag
    10921 cttatgaaaa tgggaactat ggcaacaata aatcaagaga taagttttga gattgctgct
    10981 ttggctgcca aagactatgg atttgaatta acagtagctg aaagtgatga tacagaagct
    11041 ttagaaatag aagctttaat ggaaattgaa gaagataaag aagaggattt aaaacctaga
    11101 ccaccagttg ttacagttat gggacatgtt gaccatggta agacatcttt actagatgca
    11161 ataagaaaaa ctgatgtgat ttcaggtgaa gctggaggaa taactcagca tataggtgct
    11221 tcagaagtta aaatcaatgg acacaaaata gtatttttag atactccagg acatgaggct
    11281 ttcacatcaa tgagagcaag aggagcacaa gttacagata tagcaatatt agtagttgca
    11341 gcagatgatg gtataatgcc tcaaacagtt gaagctataa accatgctaa agcagcaggt
    11401 gtacctttaa tagttgctat taataaaata gataaaccag gtgcaaaccc agataaggtt
    11461 aaacaggaat tagcagacca aggattatta gttgaagact ggggtggaga agtaatagca
    11521 gttccagtat cagctaagaa aaaagaagga atagatacac ttttagaaat ggtattattg
    11581 gttgctgaaa tggaagagtt aagagctaat ccaaataaaa gagcagtagg aactgttata
    11641 gaagctgagc ttgataaagg aagagggcca gttgcaacag tacttgttca aggtggtact
    11701 ttaacagtag gtgacccaat cgttgcaggt gttgcatgtg gtaaagttag agcaatgata
    11761 aatgcaaaag gtaagagagt aaaaacagca ggaccatcta cagcagtcga gatattaggt
    11821 ctatcagaag ttccacaagg aggagaccaa tttgtcgaag ttccaactga taagattgct
    11881 agaagtgtag cagcaagaag acaacaaata gttagagatg aaatgttaaa atcaactcaa
    11941 agattatcac tagatgctct atttagtcaa atgagtgaag gttctataaa agaccttaat
    12001 atagttataa aagcagatgt acaaggttca gtacaggcag ttaaacaatc tttagaaaaa
    12061 ttatcaaatg aagaagttca agttaaagta atacatggtg gtgttggagc tgtaacagaa
    12121 tcagacatat tacttgcagc agcttctaac gcaataataa taggattcaa tgtaagacca
    12181 gttccaggtg cagaatctct aggagaaaaa gaaaatgttg atataagaac atatactata
    12241 atatacaagg caatagaaga tatacaagcg gctatgactg gtatgttaga ccctgaatat
    12301 gttgatgaag aaacaggaaa agctgaaata agagaaatat ataaaatatc tggtgttgga
    12361 acagtagctg gatgttatgt gactaatggt aagatattta gaaattgtaa agtaagatta
    12421 gttagagata gcataataat acatgaaggt gagcttgcag cacttaagag atttaaagat
    12481 gatgtaaaag aagttaattc tggatacgaa tgtggtatga gttttgtaaa ttataacgac
    12541 ataaaagaag gcgatatagt agaagcttat ataactaaag aagtagaaag aaaattataa
    12601 attcgtatca aagaaaatca gttagtaaag aaggtcgatg gtatggcatc atataataga
    12661 acaagaagga tagcagaaga aataagaaaa gtcgtaagca ctatgcttat aaatggagta
    12721 aaagacccta gaataacatc tatggtgagt gtaactgatg tagaagttac aaatgaccta
    12781 agatatgctt atgtatatgt aagcattcta ggtggagatg aagaatccac actaacaggt
    12841 cttaaaagtg ctggaggata tataagaaga gaagtaggaa aaaatataaa acttagatat
    12901 attcctgaaa tagtatttaa acttgatgat tcaatagaaa aaggaatgta tatggatagc
    12961 cttataaaaa gagttaatga aaaaaatgct caacaaaatg aggatgaaaa ttatgatgaa
    13021 taatatggat agtattataa actatattta tgagggtgac tattttatag ttacctctca
    13081 tatcagtcca gatggagaca atattggttc tacactttca atgtattata cattgaagaa
    13141 tttaggaaaa aatgtatatt atgtacttga tgatgaagct ccattaaatt taaggttttt
    13201 ggttgaagat gtaaaaatct ataagtctaa tgaatttaat atgaagaact attctctaat
    13261 tgcacttgat tgtggggata agatgagaat atgtgtgagt gatgaaatca aaaataatgc
    13321 atcaaaatta atctgtattg accatcatgc tagtaatgat tcatatggag atttaaatta
    13381 tgtagatatt gatgcatctt ctacatgtga attagtttat aatttattag ttagatttca
    13441 gcaaataaaa gatattaata ttataaatga agatatagca acatgtttat atacaggtct
    13501 tgtaactgat actggaaact tttcgtattc aaatgtacat gcaagtagct ttgaaatggc
    13561 aaaaaattta cttgttttag gtgcacaaaa aaatactata atacaaaata tatatcaaag
    13621 caattcttct ggttattaca aattattagg agaagctcta aagggtttgg agatatttga
    13681 ttcaaaggta tctagtatag ttttaactca agacatgatg aatagaaata atattagctt
    13741 taatgatgtt gatggaataa caacatatac aagagacata aaaggtatag aagttggaat
    13801 tttatttaaa gagaaaaaac aaaatgaaat taaagtgagt tttaggtcaa aaaattatgt
    13861 tgatgtaagt gaaatagcca agttatttgg tggtggtggt catgtaaggg ctgctggatg
    13921 tactattaga gatagtattg ataatgcaaa gaaaatggta ctagaggcag tgttaaaatc
    13981 aatttaaggt gacagtaaaa tgaataagat tataagtata ttgaaaccca caggtatgac
    14041 atctcatgat gttgtaagta gagtgagaaa gattttaaat ataaaaaaag ttggtcatac
    14101 aggtactctt gacccagatg cttcaggtgt tttaccaatc tgtataggga aagctacaaa
    14161 agtatgtgaa gttattttaa acaaagataa atcgtatata tgtgagctta ctttaggtat
    14221 aagtacagat acttatgatg cttcaggtga aattttaaaa aaagttgatg attttaaatt
    14281 tagtaatgaa gacatagaaa gagcttttga cacacaaaga ggagagataa atcaacttcc
    14341 tccaatttat tcagctttaa aggtaaatgg aaagagaatg tgtgatttag taagaagtgg
    14401 tagacaatct gaaattactt taaaaacaag aagagtaaat ataaaagaca taaaaatact
    14461 aagtataaaa ggtaataaag taatgtttta tgttgagtgt tctaaaggga cttatgtgag
    14521 aagtatttgc catgatatag gagaatatct aggttgtgga gctcatatgt cgtttttgaa
    14581 tagaacatct tctggtaaat ttgacttaga taattcaata acattagaag aattagaatt
    14641 attttatgaa aataaaactt tagacaaata cttatatgat atagattatg tccttgattc
    14701 gtttaattat gttgtattaa accctaatgc tataaaatat tatagtaatg gtggctcaat
    14761 agatgataaa aggtttttaa agaataattt cgataaagat gatgaatttg taagagtata
    14821 cagtacagat aatttcttag gattaggaaa gttatcaaaa cacaataata caataagtgt
    14881 taaaagtgat aagatgttca tataaccaat gtatttaaaa aaacaacaaa agagatagta
    14941 ctataataac ttctatagtc tatctctttt acttatgcta taatagtttg tgaataagtt
    15001 aagagtaaat tatattttag cggggaacat ttattatggt gattataaaa tctataaaag
    15061 aaatagcaaa cataaaagaa agtgtcatta ccataggaaa ttttgatggt gtacacaaag
    15121 gacatcaagt tcttatagga aaaacagttg aacatgcaaa aaaagaaaat atacagagta
    15181 ttgtatttac atttgcaaat catcctgtaa attactttag accaaactca acaaaaaata
    15241 ttataagcaa tgatgataaa ataaaattat tagaagattt gggaatagat atagttgtaa
    15301 tcataccatt tgatgagtat atgactaaaa tacctgcaaa ggatttcgta gatgagatac
    15361 tagtaaaaaa attaaaagct aaaaagatag taattggaca tgactttaca tttgcaaggg
    15421 caaaagaagg caatgttaat ctattaaaaa gcttagaaca tgaatttggt tttgaggtag
    15481 aagtaataaa acccatcaaa atcaatgatg taagagtgag tagtacttat attagaagtt
    15541 tagtatccca gggtgatatg gctaatgtca aggaatattt gggtagaaat tacaagttag
    15601 aaggttgtgt tatacattct aaacaccttg gaagaactat aggattccca acggcaaata
    15661 ttgaccttaa gaataatatg ctcgttccaa agagaggtat ttatgcttct attgtacata
    15721 ttggagatga gacttatttt ggtgctacaa atgtaggcta taacccaaca gttaatggaa
    15781 aaactttgtc tattgaaaca aatattttag agtttgatag agatatttat ggggaaaata
    15841 taattgttga atttttggaa agaataagag atgaaagaaa gtttaattca atagatgact
    15901 taaaaaatca attgtataat gatacaaatt atgtgtatga aaactatgtt tgcaaaaaca
    15961 agtaatgtat gataaaatga ttaaggaaaa taaccattac tcggcatttg aatactccaa
    16021 tcaatgctta gtaataggcg tttctaaaaa aatacggagg taatctaaat gataaacaaa
    16081 tcagaaataa taaaagaata tggaagagct gaaggggata ctggttctcc agaagtacaa
    16141 atagctttat taacagctag aataaacgag ttaaatggac atttaaaaac tcataagaaa
    16201 gaccaccatt caagaagagg actattaaaa atggttggta gaagaagaaa cttattagct
    16261 tacttaaagg aaaaagatct tgaaggatat agagcattaa tagctaaatt aggattaaga
    16321 aagtaatttc attctttata aaaaagcagg tatagtgtat acctgctttt ttatagttaa
    16381 ttcattataa agtaaaatag ttaataataa aagtagttta tatgttataa aaaattataa
    16441 aatgaataat attgggtaag aatattaaaa tagtatacaa atacataatc aatttaaaag
    16501 gaaagacagg ataaatatag aaattttata acaggataat tagttggaat aaggaggata
    16561 ttattttgaa aataaagtta gtatgcgata gtttgtgcga tattccagat gaaatatcag
    16621 aaaaagatta tatagagata gttccactta cagttatttt taatgatagg gaatatatag
    16681 aaggagtaga tataaagaaa gaagagtttt ataaaaaagt aaaagaaatc aagcaaatac
    16741 ctaaaacttc acaagctaca tatatggagt ttaaagaagt gtttgataag ttcataacag
    16801 aaggttatca tataatctgt atgacagggg ctgcaaacgc atcaggaact tttcaaagtg
    16861 ctatgatagc taaaaatgat gtaaatgaaa atgaaaaaat acatatattt gatactagaa
    16921 atttatctct aggtagtgga caatatgtta taaaggcatg tgaacttctt gaagaaggac
    16981 tgggatttga agaaattata gatgaattag aaaatactcg cagtagtgta agattgcttt
    17041 ttgcaccata tacacttgat tttttaaagc agagtggaag agttccagtt gcaactgctt
    17101 tgataggaaa tatgttaaat ataaagccta tatttttctt tgataatggg gaagctaagt
    17161 tggtaaataa ggttagagga ataaagaata tagcaagtaa attggtcgat ataattttag
    17221 aaatgaatga agggtcttta gaaggaaaaa tcgtcacaat aggttgtgga gataatttac
    17281 acgattgtga aatattaaaa gatgaagtaa ataaaagaat aaaagcaaga agagtattat
    17341 ttactcgagg aggagtttct atttgctctc atacagggcc agatatattg gctataagtt
    17401 gttctaatta gtagtaatat aaggtttttg catatatatg tgaagataat tttatattgt
    17461 taatttatag tatttttgat aatatagtaa atagagtgat atacgtgttt tttaataaga
    17521 ggaggacaag cattaatgtt tgaacataaa atatttaaaa tggattttgc aggaagagaa
    17581 ctttctgtag aaataggaaa gatttgtgaa atggctagtg gtagttgtat agttcgatac
    17641 agtgatagta tggtaatggt aaatacaact aaatcagcca aacctagaga cggaatagac
    17701 ttttttcctt taagtgtgga ttatgaagaa aaattatact ctgtaggtaa gatacctgga
    17761 ggttttttaa agagagaagg caagccttca gagaaagcta tacttacatc aagattgata
    17821 gatagaccaa taagaccttt attcccaaaa ggatttagaa atgatgttca ggtagttgca
    17881 acagttctat cagtagacca agattgtaca ccagatatag tagcaatgat aggttcttca
    17941 atagcccttt ctatatcaga tataccattt aatggaccaa caggttcagt atgtgttggc
    18001 cttgtagatg gtgcatttgt tgtaaatcca aacgcagagc aaagagaaaa aagttcaatg
    18061 catctagttg tttcaggtac aaaggaagca ataatgatgg ttgaagccgg agctgatgaa
    18121 gttccagacg aggtaatgct tgatgctatt ttatttgctc accaagaaat aaaaaaaata
    18181 gttgagttta tagaaggtat agttgcagaa gttggaaaag aaaaaatgcc tgttgaatta
    18241 taccatgcag gagaagaaat aactcaattg gttcgcgaat ttgcaactga taagatgaaa
    18301 aaagcagttc agacttttga aaagttagag agaatggaaa atatggatag agttaaagaa
    18361 gaaactttag ctcactttga agaaacttta gaagattttg aagattttgt tggagatata
    18421 gaagaagtat tacaagatat aataaaagaa gaagtaagaa aacttatagt acatgaaaat
    18481 gtaagaccag ataatagaaa acttgaagaa ataagaccta tatggtgtga aactggcatg
    18541 atacctagag ctcatggttc agccatattt actagaggac aaactcaagt acttaatgtt
    18601 gctactttag gtgctttagg agatgttcaa aaactagatg ggttagatga agaagaaaac
    18661 aagagatata tgcatcatta taatttccct gcatatagtg ttggagaagc tagaccatca
    18721 agaggaccag gaagaagaga aataggacat ggtgctttag cagaaagagc tttattacca
    18781 gtaataccat ctcaagaaga atttccttat gctatcagat tagtttctga agtattaagt
    18841 tcaaatggtt ctacatctca agcaagtgta tgtggctcaa cattatcttt acttgatgca
    18901 ggtgtaccta taaaagatat ggttgcaggt atagcaatgg gacttataaa gcatgatggt
    18961 aaggttgcag tgctttcaga tatacaaggt atggaagacc atttaggcga tatggacttt
    19021 aaagtagctg gaacagagta tggtataaca gcaatacaaa tggatataaa aattgatggt
    19081 atagataaag aaattctaca acgtgcttta aaacaagcta aagagggtag aatccatata
    19141 ctaggagaga tgagaaaaac tatatctcag ccaaaaccag aactttcacc atatgcacct
    19201 aagattgtta aaatgcaaat aaatcctgac aagataaaag atgtaatagg acctggtgga
    19261 aagataataa ctaagataat agacgaaact ggagttaaga tagatattga acaaactggg
    19321 gaagtattta tatcaggtat tgagattgat atgattaaga aagctcaaga acttataaat
    19381 aatatagtag ttgaaccaga agtaggaaaa acttataaag gtaaggtaag tagaataatg
    19441 aactttggag catttgttga gatacttcca ggaaaagaag gattacttca tatatctcat
    19501 atagctcatg aaagagttgc caaagttgaa gatgtattga atatagggga tgaagttgaa
    19561 gttaaagtta cagagattga tgaaaaagga agagttaatt tatcaagaaa agttctttta
    19621 ccaaaaccag aacataaaaa taaataactt attatataat taaaagcaaa gtcttaggat
    19681 tttgcttttt taaatacaaa aaagtagata attttaaaat gatagttatg gatggcttat
    19741 agttcttttt ctgtcaggct ttactcttat aaagaagcta tttaaaagct gattaataga
    19801 gagctttatt gatatatata gaatcactta agttatattt agtgcataaa cagagcttat
    19861 ttttaatagt attaaataag aaacaatttg tacataatta gggggagttt taatgataat
    19921 gatggtactt aataagagta agctcaaaaa aatcataata ctaattttag tagtgttatt
    19981 agtattatca ggaggggtaa tggtattaaa gttatttaac aacaagccag tatttaattt
    20041 atttaatggt gtggatttac catatgaaag aggtacaaag gataaaagtg gatatgttgc
    20101 attaacctgt aatatagacc ttggatggga aacagagtac gtagaatcca ttttagaaac
    20161 acttaaaaaa gaaaatgtaa agattacatt taatgtaact ggaaaatggg cagaaaagaa
    20221 taaggatgaa ttattaaaga taaaaaaaca aggacatgaa ataggtaatc atggatacaa
    20281 acatttagat tattctacat tatcatatga agacaattat gagcaaatag agacctctaa
    20341 aaagataatt gaagaaatta taggtgaaaa aactaaattt tttcaagcac cagcaggttc
    20401 ttttggtcca gaaacagtaa aggctgctaa agcattaggg tatacttcta tcaaatggga
    20461 tgccgatact atagattgga aatacaaaga ccaaccagag gtaatcattg atagaatgaa
    20521 gaaaaaagac attaaagata gtagtataat tttaatgcat ccaaccaatg caacgacaaa
    20581 atgtatagat gatattatag ctattgtaag agaaaaagga ctaaaaccag gaaaacttag
    20641 tgatgtattt aagtagacaa agtataaata tttggttaag attgtataag ttgtagttga
    20701 attaattaaa agactgactt ataatgtaat acatataaaa aataaatata attaaaacat
    20761 agtgttttca ataatccaat atgaaacaaa gaataaattg ttaggagaat gtaatgtata
    20821 agacaaaaat actggaaaat gggcttacaa taattggtga agaaatacca tatctaaagt
    20881 caataacatt aggtatttgg ataaatgcag gctctaggat agaagaagca caggtgagtg
    20941 gaacttctca ttttatagag catatgatgt ttaaaggaac caaaaacaga acatcaaaag
    21001 agattgctag ttcaatagat aatttgggtg gtcaaataaa tgcatttaca agtaaagaat
    21061 gtacatgtta ttatgtaaaa ctaatagatg agcatataga tacaggaatt gatgtattga
    21121 gtgatatgat actaaattct aaatttgata aaaatgatat agataaggaa agacttataa
    21181 tattggaaga acttaagatg tatgaagact ctccagatga cttatcttat gatttattag
    21241 tggaaaatat atatgctaat gatggattag gaatgaatat aattgggaca aaagaatctc
    21301 tgtacaatat aactagagaa tccatgttag aatacttaaa taagtattat attccaaata
    21361 acgctgttat ttctatagct ggaaacttta actttgatga tatggtggag aaaatcaaat
    21421 caaaatttgg acactgggaa aagaaaaatt taagtataga cataagtgag gctaagttta
    21481 atccttgctt tatatcaaaa aataaagata ctgaacaggt gaacttagct atgtgcctaa
    21541 aaggtattcc ttttgaaaat gatgaagaag tctattcaat ggcagtggta aataatattt
    21601 tcggtggaag tataagctca aggctttttc agaaaataag ggaagaaaaa ggtttagtat
    21661 attcaatata ctcatcacag acattatata ggaaatgtgg tgaattaggt atatttgcaa
    21721 gcatgagtac agaaaattta caagatgtat ataatttaat aaagaaagaa attgaaaata
    21781 taagagaaaa ctatcttaca gaaaaagaaa tatctgaaag caaggaacag ttaaaaggaa
    21841 attatatatt ggatttagaa agtactagca gtagaatgat gtctactggt aaatctatgt
    21901 tacttagtaa aaaagtaaaa actacagatg aaatacttga atgtataaat aatgttaata
    21961 ttaattcaat aaagaaggtt gttgataagg tattcaatat cgaaaatatt ggtacatgta
    22021 tagttggaag agatgtagaa aagattctac acttggatta atattgaaaa aagtaaatct
    22081 ctggaggtat aaaatatgaa tttatctgaa atagcaggta aggaaatagt taacttggtc
    22141 acaggagaaa gattgggggt cattggagaa tgtgacctta tactagatga aactacaggc
    22201 ggtatattag cgcttctaat tccaaaagaa aaaggatttt ttgccagaag aaaagataag
    22261 tcatttttag aagtaccatg gagaaatgta aaaaaaatag gtaatgacat gataattata
    22321 gaatatgagg gatatattta ggaggaaaaa atgagtattt tagtgcaaaa gtttggtggg
    22381 acatcagtag aatcatatga aaaaatgaat gaagtatgta agatagtaaa agcatacaaa
    22441 aaaaatgatg aagaattaca attagtatta gtagtttcag caatgggaag aaaaggagct
    22501 ccatatgcta cagatacact tataaatttg tgtagtgcag taaatgatga gccaagtaaa
    22561 agagaacttg atttaataat gtcttgtgga gaaataatat caggaacaat tcttgctaat
    22621 ttattgaatg cacaggggat agattctgtt ttctttactg gtcagcaagc aggtataatt
    22681 acaagtgatg aatattctaa tgcaaagata aagtatataa atcctaaaaa ggtaaaaaga
    22741 gctttagatg atggtaaggt tgtagttata gctgggttcc agggtgtaac agatgatgga
    22801 gaaataacta ctcttggaag aggcggaagc gatacatcag cagttgctat tggtaaagct
    22861 ttagaatgtg aaacagttga aatttacact gatgtggatg gtataatgac tgcagaccct
    22921 agagtagaac caaacgctaa agtattgagt tttatagatt atgaggaagt attccaaatg
    22981 gctgacaaag gtgcaaaagt aatacatcca agagcagtac agttggctaa aagtggaaat
    23041 ataactttag ccataaagaa tacaatgaat ccaacttttg aaggtacaaa aataggttct
    23101 ttatgtagac acttagaaga caatatagaa tatgaacaag aaagagattt taaggttgct
    23161 gttgcaaata aagatagcgt agcacaagtt aagattaagt cagcagaaga agtgtttaca
    23221 gaagtattaa atgagataga aaagaaactt ataactatag atatgataaa tttctttata
    23281 tcagaaaaag catttgttgt agaagatgct gatataaaat cattaaagga aatactagat
    23341 aaatttgaat tagattatga agtaaataga gactgtgcta aggtaacatt gatatgttca
    23401 agaattgctg atgaaatgtc tggtatcatg tcaaaggtag ttagaggatt atcaaaagca
    23461 ggggtatctc ttttacaaac atcagactca aatatgacta tatcttgttt agttagtgaa
    23521 gaagatatgc atacagcagt acatgctata caccaacaat tttatttaaa ataaactgta
    23581 taatacaatt attttttaat caaatttaag aatttcatat atatcttaat atatttgatt
    23641 tatttggtaa aactaaatta aatataattt tgataggagt ttttttatga aagattatag
    23701 agaagataat gaaagatttg ataaagataa ttgtagtgaa gaatctagtt gcgaagagag
    23761 agaatgtata aagcaatttg gtaccaatga aatgccacct caacctccta aagatattca
    23821 atgtataact ataattggag aaattgaggg acattttata ggaaatcctc aaaaaaaagc
    23881 tacaaagtac gagcatataa ttccaatgct atactcaatt gaggaaagta atgatgttaa
    23941 aggagtgtta gttgttttaa acactgttgg aggagatatt gaagcaggtt tagctattgc
    24001 agaactttta aacagtacat ctaaaaaagt agttactctt gttctaggag gaagtcatag
    24061 tataggtgtt cctcttgcta cagcaggaga ttactcattt atagcaccaa cagcaaccat
    24121 gattatacat ccagtgcgaa ctactggtct tgtgattggg ataaatgaga cttttgagta
    24181 ttttaaaaag atgcaagata gaataattca atttataata agaacatcaa atatcaaaaa
    24241 agatgtactt gaaaaactta tgcatgaaaa agatgaactt gtaagtgatg ttggtagtgt
    24301 attaatagga aaagaagctg tggattatgg tcttatagat gaagttggag gactaaaaga
    24361 agcacttaaa aaattaagag agcttataaa agaatctgag gaagaaaaaa ataatgatta
    24421 aaatttgttt aaataataaa atttatgata taatgatgta ataaagtagg agtgttagtg
    24481 ttaaggcatt agcacttttt gctgtgaagg ggtaaaactt gctaaaaata aggagtgata
    24541 ttaatggcca cgaaaaagaa gaaaaaaaag aaggtaaata ccttaagttt taatgcagaa
    24601 tatcataatt taataacaat atttataggt gtatttttat tatatagttt aaattctaat
    24661 tctatgggat ggatacctgt gttaatgcaa aatcttttta agggactatt tggagggctt
    24721 tcaatagcaa taccatttat agtaattata acaggacttt taggattttt tgatggaaat
    24781 gaatatatat ataggctaag aaagactaaa ctatactaca taattatttt atttatattt
    24841 gtattttatg gattattaaa tgcaggaact cttcctgtag atagcccact aaaaggtaac
    24901 atgtttgatg atgttatgaa attgggtgta agtggacaag gtagtggttt gatagcaaca
    24961 actattgcgt attatatgag taaaatattt ggtatagcag gtggatggtt aataagtatc
    25021 ttcgcactta tactttctgt aatgtttata tttaatattt ctataaaaga tttggtgtca
    25081 aatgcaaaat caaaatctaa agcatctaaa gacagcaatt taacgtttaa agataaaatt
    25141 gcaaatatga aaaaatctgc aatagatatg atgactgatg aagtagatga tactactata
    25201 aataaaaaac caggtttctt taaagggtta atgtcaaaag gtcttaataa tgatgatgaa
    25261 gaagatgaat attttgaaga agaaaatagt gatggtgtag atgataaaac tataaaaata
    25321 gttggattta ataaggcaga agatgagtat ttagaaatat tagaaggaac tcaaagtatg
    25381 ccagagttag atgttttaaa agaattacaa aaagctacca atgaaaatcc agttgtagat
    25441 actaaaccag aaaaaaaagt tgatatagct aaacctaatt taaatataga aaaaactcaa
    25501 cctatgagta ttgtagcaga gccagttaat gaggattatt caaactataa aaagcctagc
    25561 attgagcttt taaataaagt aaataagaag tctgatgaaa atggtaaaaa gaaagtgttg
    25621 aaaaatgctt ctcttttaga aaagacacta tcagattttg gagtggaagc aaaaattaat
    25681 caagttacag ttggacctac tataacaaga tatgaaatac aaccaagtcc aggagtcaag
    25741 gtaagtaaaa ttgtaaattt aactgatgat atagctttaa gtttagcagc taaaagtata
    25801 agaattgaag ccccaattcc tggtaaaagt gcaattggaa tagaagttcc aaatgaagaa
    25861 gctcaaatgg ttggtgtgag agaagttcta gaaagtgaag aatttaataa ttttaactct
    25921 cctcttgcaa tgggacttgg aaaagacgta gcaggaaaaa ttataattgg agatataggt
    25981 aagatgccac acttactaat agctggttct acaggttcag gaaagagtgt gtgtgtaaat
    26041 acattaataa gtagtatttt atataaggca aatcctgatg aagtaaaatt actattaata
    26101 gacccaaaag tagttgaact tgcaaattat aatggaatac cacatctatt gataccagta
    26161 gttactgacc ctaaaaaagc tgcaaatgct ttaaattggg cagttacaga gatgaataga
    26221 aggtacaaat tatttgcaga tgctcaagta aaagatgtaa caagttataa tgagaaagcg
    26281 gaagaaaaac ttcctaaaat agtaataata atagatgagt tagcagattt aatgatggca
    26341 agtgcaaatg atgtagaaga ttatatttgt agattagctc aaatggcaag agctgctgga
    26401 atgcacttga ttgtagcaac tcaaagacca tctgttgacg ttataacagg ggtaataaag
    26461 gctaatatac catctagaat agcatttgca gtatcatctc aaactgattc aagaacaatc
    26521 cttgatatgg gtggtgcaga gaagcttcta ggtaaaggtg atatgttatt ctatccactt
    26581 ggtgcagcaa aaccagttag acttcaaggg gcattcatat cagagagtga gtcggaaaaa
    26641 gtaatcgatt ttgtaaaaag tcaagttaaa gatggaataa aatatgaaga agatataatt
    26701 gaaactatat ctaaggttaa tacttcaaag ggtagtgatg aggatgagtt tttgagtgaa
    26761 gcgatagaat ttgtcgtgga aagtggtcaa gcatctgcct ctatgctaca gagaagattt
    26821 aaaataggat tcaatagagc agctagactt atagattcta tggaagaaag aggaataatt
    26881 ggagcaagtg agggaagtaa accgagaaag gtattaataa gcaaacagga tttgcagaat
    26941 ttagaaggtg aatagaattg tcagttataa aaatagaaaa agcacttaag ctagataaag
    27001 taatatttgt agatgtgaga actgaaggag aatatgaaga agaccatata ttgaatgcaa
    27061 tcaatatgcc tctttttaaa aataatgaac ataatgacgt tggaacaatt tataagatgc
    27121 agggaaaaca tgaagcaata caaaaagggt ttgactatgt atcgtataaa ttaaaggata
    27181 tatatttaca agcagcagaa cttgcattaa attatgataa tattgtcata tattgtgcaa
    27241 gaggcggaat gagaagtggc agtatagtaa atttattatc atcattaggt gtaaatgtat
    27301 atcaacttga aggtggatat aaggcttata gaaattttgt attagagtat ctaaggcatg
    27361 taatggatac aaaaaacttt gtatctgttc atggattgac tggtacagga aagactgatt
    27421 tgttacattt attagatgaa aaaggaatag atgtactgga tttagaaggc atagctaaaa
    27481 acagtggttc tacctttggt tttataactt ttgataaaaa gccaccttct caaaagaact
    27541 ttgaaacaaa actatttgaa agtatatttt tttcgaaaga gaattatata tttgtcgaga
    27601 gtgaaagcaa acgagttgga agtgttacag ttccaaatga aattttcgag gcaatgacac
    27661 gagaaggaca tcatatatta ttagaatgtg atattgaaaa tagagttgat agactttgta
    27721 gagattatat ttatgataaa gatgaaggta atatattgat tttaaaagaa tgtataaaca
    27781 agtttagaaa aagattgggt aataaagtag tagatgatta tattgattta ttagaatcag
    27841 gtgaatataa agaacttgta aaaagatatc ttttagaata ctacgaccca ctttatatgc
    27901 attcagtaga aaaatataag tatagtaaga ttataaattt taatgataca caaaaagctt
    27961 tagaagatgt tatagactta tatgagagta tattggaagg agaaagtgaa tgttaaaaat
    28021 agcattagaa tcattaggat gctcaaaaaa tttagtagat gcagaaataa tgatgggaat
    28081 tttaaataac aaaggctata aattaattgg agattttgaa gaagctgatg taattatagt
    28141 aaatacatgt ggatttatag agtcagctaa acaagaatct atagatacga taataaactt
    28201 tgcagagtta aaaaaaactg gcaatttaaa gttgttgata gtaacagggt gtctagccca
    28261 aagatattct gaagaattaa aaactgaaat tcctgaaata gatgctatag taggaacagg
    28321 aagctatcaa aatatagata aaatacttaa agaattaagt gaaatgcata aaattgtaag
    28381 ccttaatgat atagagtttg tatttaatga agatttacca agatatatat ctacacctag
    28441 ctatatggcc tatttaaaaa ttggagaagg atgttccaat aattgtactt attgtataat
    28501 accaaaacta agaggtaaat atagaagtag aaaatttgaa gatataatta aagaagctaa
    28561 gaaattagct gaatctggag taaaagaact tgtggttata gcacaggata cgacaaagta
    28621 cggttttgat ttatatggaa aagaaagatt atcagaactt ttagaagaac ttgcaaaaat
    28681 agatggattt aagtggataa gggttatgta ttcatatcca gaatctataa cagaagaatt
    28741 gatacaagtt ataaagaaat atgataatat atgtagttac tttgatatgc caatacagca
    28801 tgcaagtaac aatattttaa aattaatgaa tagaaaaact actaaagaag atatattaaa
    28861 taaaatcaat ctgataagaa gtaatatacc agatgctata cttagaacta caataatagt
    28921 agggtttcct ggtgaaacag aagatgactt caaacaactt gtggactttg ttgaagaggt
    28981 taaatttgat agacttggtg catttgcata ttcaagagaa gaagatacac cagcagatag
    29041 attgccaaac catatagatg aagaagtaaa aatacaaaga agagatactt taatgatgat
    29101 tcaacaaaaa atatctgaag aacttaatga caaaaagata ggaaaaactt atgaggtgtt
    29161 aatagaagaa cagatagaag ataatgtata tacaggaaga acacaaggtg atgcagaaga
    29221 aattgacagt atagtgtatg taaaaagtgt agacaatctt gaagtaggag aatttgtaag
    29281 tgttcaaata aatgatgcaa tggaatacga tttaatggga gatgttttat atgaacttgc
    29341 ctaacaagtt aactttgttt agaatatttt taataccagt atttgtttta attatgttgc
    29401 tcaatatacc aaataaattt ttaatagctt gtattatttt tataatagct tcaattactg
    29461 atgctttaga tggaaaaata gctagaaaat acaatctagt cactgacttt ggaaaattta
    29521 tggacccatt agcagataag ttattggtta tttcagcact tacatgtatg atagaagacc
    29581 atcttgtaag tagttggatg gtaattataa tagtggctag agagcttaca gttagcatat
    29641 taagagctat agctgctgct gatggaaaag taattgctgc tggaaacagt ggaaaactta
    29701 aaacgataac tcaaatggta tctatagtgt ttttattgtt aggtgctcag tttgaaaatg
    29761 tattgatatt aaatattggt gaaattctca ttcttatagc tacactactt acattatatt
    29821 ctggatggga atatctatat aaaaataaag agctttttat gtcttctaag taaaaaatta
    29881 tgctttaata aataaaaact aatgattaat aaataaaaaa taacaactta aaattttaaa
    29941 aatgtaaaaa agggtatact attatagtat acctcttttc attttaagaa tattttcttt
    30001 tgtgtatata attattaata atggtttata aataaaatgt aatgaataaa aatataatca
    30061 ttagtagcgt ttttaaattg actaggtttg atttatgtta tataattaat atataacaag
    30121 agaacaaatg tttgtagata aatgaaggga tggtatagag atgagtgtag atcaagaaaa
    30181 attaaaagcg ttgaatgaag ctttaggtaa aattgaaaaa gattttggta aaggttcagt
    30241 aatgaaattg ggagaagcaa catctatgtc tatagatgtt atatcaacag gagcgattgg
    30301 tttagacata gctattggta taggaggtct acctagaggg agaatagttg aagtatatgg
    30361 tccagaatct tctggtaaga ctactgttgc gcttagttgt gtagcatcag ctcaaaaaga
    30421 tggaggaata gctgcattta tagatgcaga acatgcactt gacccagtat atgcaaaagc
    30481 tttgggtgtg gatgttgata acctaataat atctcaacca gatacaggtg aacaggcttt
    30541 agagatagca gaggcattga taagaagtgg agcgatagat ataatagtaa tagactcagt
    30601 agcagcatta gttccaaagg ctgaaataga tggagatatg ggtgattctc atgtaggttt
    30661 acaagctaga cttatgtcac aagcacttag aaagttaact ggttcaatta aaaaatcaaa
    30721 ttgtgttgct atatttataa accagttaag agagaaagta ggaataatgt ttggaaaccc
    30781 agaaactact actggaggac gtgcactaaa attctattca tcagttagat tggatgttag
    30841 aaaaatagat acaataaaac aaggtgataa agttataggt agtagaacta gagttaaagt
    30901 tgttaaaaac aaagtagcac caccatttaa gcaagctgaa tttgatataa tgtatggaga
    30961 agggatttca aaaattggag accttttaga tatagctgct gatgtagata tagttaaaaa
    31021 atcaggttca tggtatagtt acaatgatac taaacttgga caaggaagag aaaatgttaa
    31081 aaaattcttg gaagataatt tagatttaac tactgaaata gatgagaaag ttagagcatt
    31141 ttacaattta aatgaagaac atgaagaatc aggtacttca gtatcaaaag aaattgtaga
    31201 agaataagta aaaatacaaa aaaataacta gtaattaaag acatgccacg ctccctatta
    31261 gcttgacaca atgttgaaaa aattataaaa ttaagtgagg ataaaatatc taaatttaat
    31321 tttaattatt aggaatattt tttaatcata gtgatgacat tttgggatta ttaacgaagg
    31381 aggtggatgt catagatagt gtagtaatga ttgtaatagg tgcagctgtt ggtattatag
    31441 ctggatattt tgttagaaaa aatatatctg aagcaaaaat tggacaagct gaaaacttgg
    31501 ccaaagaaat aatagataaa gcacaccacg actcagaaac agtacaaaaa gaaaaattac
    31561 tagaagctaa agaagagatc cataagtgga gaacagaagc tgaaagagaa aacagagaaa
    31621 gaagaacaga agtacaaaag tatgaaaaaa gagtagtaca aaaagaagaa gtcttagata
    31681 gaaaattaca aaatttagaa tctaaagaag taaacctaag tgaaaaatta aaggtagtgg
    31741 aaaagaaaga agaagaagtt gaagtaataa aaactcaaca gttagaaaaa ttagaaagta
    31801 tatcaggaat tacttctgat aaggccaaag aaatcatctt aaccaatgcg gagagagatg
    31861 taagacgtga aatgtctata atgataaaag agatagaaag tcaagctaaa gaagaagctg
    31921 ataagaaatc aagagaaatt ataggatatg caattcaaaa atgtgcagca gatcacgtag
    31981 ccgaaactac tgtaacagta gtaagcttgc ctaatgatga aatgaaaggt agaataatag
    32041 gtagggaagg tagaaatata aggactttag aaaccttaac aggtatagac cttataatag
    32101 atgacactcc agaagccgta attttatcag gatttgatcc aataagaaga gaaattgcaa
    32161 gaattgctct agaaaaatta atagcagatg gaagaataca tccagctaga attgaagaaa
    32221 tggttgaaaa ggcgagaaaa gaagttgaca atattataaa agaatatggt gaacaagctg
    32281 catttgaaac tagtgtacat gggattcatc cggaattaat aagattatta ggtagactta
    32341 actatagaac tagttatgga caaaatgtac ttaaacattc tatagaagtt gctcatatag
    32401 ctggtataat ggctgctgaa ataggagcag atattagatt ggctaagagg gcaggtttat
    32461 tacatgatat aggtaaggct gtagaccatg aaatggaagg tacacatgtt gaaataggta
    32521 tggatttact tagacgttat aaagagtcta aagaagttat acatgccatg agtactcatc
    32581 atggagatta cgagcctcaa acagtagagg ctgtattagt tacagcagca gatgcaatat
    32641 cagctgctag acctggtgcg agaagagaaa ctttagaagc atatataaag agacttgaaa
    32701 aattagaaga aatagctaat tcatatgaag gtgtagaaaa atcatttgct atacaagctg
    32761 gtagagaaat aagaataatg gtaaaaccag aagctataaa tgatgaagaa atacatctat
    32821 tggctagaga tatgactaag aaaatagagg atgaacttga atatcctgga caaataaaag
    32881 taagcataat aagagaaaca agagctatag agtatgcgaa gtaaaagaaa taatatctaa
    32941 ataataagaa aatattttta gaaatttaaa tttgccacaa gatatgataa tatatcttgt
    33001 ggcttttaaa ttgtttaagt ttaattttat ataatgcaaa aataatctaa gtgctaaaag
    33061 tgctacttac aaaagattaa taaaataaag ctttatatat gtattattta tataattatc
    33121 caagtttgtt tgatataatg aagttgtaaa ataaatatat tactacttgg aatgcttgta
    33181 cttaaataag atgaaaatac aatgacgcta aagtgaggat tagagagagg gtacactatg
    33241 aaaaaaaata cacttttaca tacaaatatt atggtttgca ttattattac tcttggtttt
    33301 gtggctacct ctataattgg atatcaatct aataccaata tttttaaaaa tgatattgaa
    33361 catgtatcta cattggctgc tgaaggtatt tattatcaga tagataaact actttcagaa
    33421 cctataaatg tatctcttac aatggcaaat gacagtctat taaaaaattt tttagatggt
    33481 gagaaagaac atttaaatga tgaagaattt atttataaat tacaagatta tctgaatgtg
    33541 tatagaaata aatattcata tgattctgtt tttttagtat caactaaaac caataattat
    33601 tatcatttta atggacttga tagaaatctt agcgctaata actcagagaa tcaatggtat
    33661 tatactttct taaagaatga tgatgaatac tcattaaatg ttgataatga tgaagcaagt
    33721 aacaatagta ttacagtctt tgtaaattgt aagataaaag atgataatgg agcaactatg
    33781 ggaattatag gtgttgggtt aaaggttaat tcattacaaa tgttgcttaa aggatataat
    33841 gacaaatttg atgttgttgc acgcttaatt gatgataaag ggtttgtaca attagcagta
    33901 gataaaaccg gtcatgagaa tataaatttt tttgaaaatt ctagtagcga cttgtctaat
    33961 tcgaaacaat taatattaaa caacaaaaag gaacaaaaga gtttttggta ttcatctgaa
    34021 aaatcaaaaa gttatattgt atgtcagtac atacctagtt taaaatggca tcttgttcta
    34081 gaaaatgatt ttactttaat gattaaacag cttcatctgc aattttttaa aggcattgca
    34141 gttatagttt taattataat atttgtactt tttactatta cacatgtgct taaaaaatac
    34201 aaaaaccaaa taataaagct tagtgtttca cagaaattgg aatatcaaaa attgttgagc
    34261 aaatctactg aaggtcttta tgaaaatatt tttgaaattg atattactaa caataaagct
    34321 ggttgtgaag atgagaatag gtattttcat gaatttggat tagggcttaa taccccatat
    34381 gatgaagcct tacatacaat agctaaaaac caaatcaaag aagaatatgc acaaggttat
    34441 ttagatacat ttttacctga aaatgtaatt aaacgttata atagcggaat taataatatc
    34501 agctatgatt ttttaattaa aaaagatgaa gaaagttatc attggattag aattaatgca
    34561 cgaattttct tttggacatc agatgaatct gttcgtatga tatcttatcg taaaaatata
    34621 gatgaagaaa aaaatagaga attactactt ctcgaacgtt cacaacgtga ttctcttaca
    34681 gggctttaca ataaagctac aacagaggaa tttattcgtt catttttaaa aaatgaaagt
    34741 aatcaagatt tagaacacgt ttttctaatt ttcgatattg ataattttaa aaatgtaaat
    34801 gataaatatg gtcatgtatt tggagacttt gttataagtg aatttgcctt agaattaaaa
    34861 tctcaatttc gtgaggacga tattgtggga cgtattggtg gagatgagtt tgtagtatta
    34921 ataaaagact ttaataataa taactatgtg cttatagaca aattagagcg tttttgttct
    34981 aagattaatc agaaaaaatt tgttgagcaa gcagaatttt ctattgcatg tagtattggt
    35041 gtagcaatgt atcctgaaca tggatatgta tactctgaac tatatgagaa agcagaccaa
    35101 gcactttact atgcaaaaag ccatgggaaa aattcttatc atatattttc agaggattct
    35161 attgatagta tttcatttca tatagactct agagatataa aagtattatt agaaatggct
    35221 actgatggtg ttgtcaaatt tgcttgtatg gaaaacttaa aattacttta tttcaaccaa
    35281 aaaatgcttg aatttactgg atattcagct caaactgtta cttctatagg ttttgatatt
    35341 ttttcgcctg tattacaaga cgacttacaa atgggactag atacattaag agaagcaatt
    35401 tctaaaagag agacttttga aatagttttt cgtttttgtc atacagatgg tcatattttt
    35461 cctgtaagga tgcaaggtaa gtttgtaagt gaattatatc aaaatcgtta tcctgttttt
    35521 tatgcgattt atacagacat atcaagtatc aatactttac tagaaaataa atagtaaata
    35581 ctacagtaaa atct
//
LOCUS       DAELLV010000014        34346 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.014, whole genome shotgun sequence.
ACCESSION   DAELLV010000014 DAELLV010000000
VERSION     DAELLV010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34346
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.014"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(69..1232)
                     /locus_tag="KPD58_000618"
     CDS             complement(69..1232)
                     /locus_tag="KPD58_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBG0264405.1"
                     /translation="MKKLASTALAILIALTPLSFSFANNKENADANQLNISSKSAILM
                     DVGSGQILYEKDAHKKLPPASVTKVMTMLLIVEALDSGKIKLDDEVQVSETASSMGGS
                     QIFLEPGETQKVDTLLKGIAVASANDACVAMAEHLAGSVEGFVDRMNAKAKELNMNDT
                     HFANTNGLPVANHYTSAHDIALMSRELLKHEMISKYLTTWMDKVVVGKKQVTVGLANT
                     NKLIKHYQGATGVKTGFTQEAKYCLSASAKRGNTHLVAATLGAETSPERFNDASSLLN
                     YGFANYESVKLCSKGDNIATLTLDKADENKVKLVAKEDLNALIKKGSSKEFEKKIEIV
                     KNPKMPIKKGTVLGKIKICKDKKVIGEVELINTKDINKASYLQMLQRIIDNMI"
     gene            complement(1286..1486)
                     /locus_tag="KPD58_000619"
     CDS             complement(1286..1486)
                     /locus_tag="KPD58_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protein"
                     /protein_id="HBG0264406.1"
                     /translation="MDDVSRQNAIKALKQTKMEIAGEYGMNYEDAFEIIENASNKGVL
                     EGYFKKLEKKKNLGQGISRHLE"
     gene            complement(1692..3368)
                     /locus_tag="KPD58_000620"
     CDS             complement(1692..3368)
                     /locus_tag="KPD58_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBG0264407.1"
                     /translation="MQLFKKNTNKIKVMALGGLNEVGKNMTVVEYKDEIIVIDAGLSF
                     PEDEMLGVDIVIPDITYLVKNRDKIKGIFITHGHEDHIGALPYILKKINVPVYGARLS
                     IGLIQVKLKEHKMNNVKLNVIGPRQVIKLDNMEVEFLKNNHSIPDAYSIAIHTDQGII
                     YHTGDFKIDLTPIDGDVMDMHRICELSKKGVLLMLADSTNAEKPGFTMSEKTVGVGLD
                     ELFAKGNGRRIIVATFASNIHRLQQIINTAEKFNRKVAISGRSMVNVVGVAKELGYLD
                     ISDDMLIDLNDICKYEDSELVIITTGSQGEPMSALARMAFSEHKKVEIKSGDLVIISA
                     HPIPGNEKLISRVINFLFEKGAEVVYSDIADIHVSGHACQEELKLIHALVRPKFFMPA
                     HGEYRMLKRHAEIAEQLGMDKENIFVMQTGDVLELDKNSAKVANRIQTGNILVDGLGV
                     GDVGNIVLRDRKHLSEDGLMIVVVTISKDEGKVLAGPDIISRGFVYVRESEDLMDGAK
                     DIIKNVLNECEEKNIKEWAYLKNNIKENLKEYLYQKTKRNPMILPIIMEV"
     gene            3585..4322
                     /locus_tag="KPD58_000621"
     CDS             3585..4322
                     /locus_tag="KPD58_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBG0264408.1"
                     /translation="MRGKTHCAIGILTAIQTSLIFKIPISLVDILVSATFAVLPDLDK
                     SNSMVSNFILKNNVSKYIYRIFIYAVNIIIFFISININDNFYLSAIITFVAIIIIEAK
                     LTHTFLRKVFLSLIFILLAICLYIIEVEIYFTIFCLMLSIFPWLKHRSFSHSIFATII
                     VYFLLKQIELITNINNLSFYGTIGYASHMFLGDLFTKQGIPIFYPLSEKKISLGFLTV
                     GGPFSNFIEKSFIFVLIGLIIFSILKL"
     gene            complement(4439..4897)
                     /locus_tag="KPD58_000622"
     CDS             complement(4439..4897)
                     /locus_tag="KPD58_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBG0264409.1"
                     /translation="MANTMDLLKDKLKETGFKITPQRRAIVEILLKHDHSHLSSEEIY
                     DLVRVDCPEIGLATVYRTMQLLDEIGLISKLNLDDGCIRYEISLHKEDCHNHHHLICK
                     NCGKIMEAKEDLLDNIEKEIQSLYKFKILDHDVKFYGLCDECNGVSDSEE"
     gene            complement(5179..5466)
                     /locus_tag="KPD58_000623"
     CDS             complement(5179..5466)
                     /locus_tag="KPD58_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HBG0264410.1"
                     /translation="MEENIINLIDENGVESQFEIILTLEAEGKEYAILMPLDDEEAEE
                     ALIFRIDEDEEGEILIPLESDEEYETVVAVYTAIMEEEGLNYDEDESNGLN"
     gene            complement(5544..5969)
                     /gene="ruvX"
                     /locus_tag="KPD58_000624"
     CDS             complement(5544..5969)
                     /gene="ruvX"
                     /locus_tag="KPD58_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBG0264411.1"
                     /translation="MLDGRIMGLDVGDKTIGVAVSDLMGLTAQGVKTIKRVGKKKDIE
                     ELKAIIKEKQVNKIVSGLPKNMNGTLGPQGEKVIKFCELVKAETGIDVEFWDERLSTV
                     AAERSLLEADVSRQKRKKVIDMLAAVIILQGYLDFKINS"
     gene            complement(6175..8346)
                     /locus_tag="KPD58_000625"
     CDS             complement(6175..8346)
                     /locus_tag="KPD58_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264412.1"
                     /translation="MRKKIMSLFAIVIMIFVSTASYGFAEEKGKVIFIDMNRTNLGSM
                     MNIPILKNEIEKRGYVALMNIRGDQGTDDRRSYASMGAGTRASVTTEDYINFQKVDQD
                     TAKAFEVATGQKAKEINDLGINRSINENLEYGEYGANLGSLGKTLSENGLKTSVIGNA
                     DIIENNQLVKNRNLCLVAMDQYGRIDSGNIDNINKKDSKMPFGISTDYDKLLDETKKS
                     YKNSDAIFVELGDTYRLDMYRLNLNDNTYDTMKKNIVKNINKYLEQVFSMVGENDTVY
                     IASAFPSDLDYKNKRRLSPIIKFSGEGKGLLESATTRKAGIVANLDVGVDILGTFGLE
                     NKEMVGRSYTLVNKEDNISYLKSLYERIVSISSIRSTIINGFVGVISASWVIAMFAIF
                     FRNYMPHKERVFKVLKEFIKLGIIMPLSFMLAPLMNFKTPISISLGIFATTLILYLLG
                     RKLFKDNDIKHMGFFATLTILVIVIDSVFGTYLMQNNIMSYDAVIGARYYGVGNEYEG
                     VTIASAVFGLAVLLHYKKVSKLLVVIFSLIILITSAYPSMGANVGGAISECVAYLLFI
                     MLIYDIKIDFKKAVLLAVSAVVVVFAFAALDILSGSESHLGMFVQRIFLNGPGEILQT
                     FGRKIQMNMQLAQTSVWVNILLVGIGVIAVLIFKPSRHFRKIMNNFPILFKGFIASMV
                     GCIITLLVNDSGIVAASTASIYILIPLIIISINMIIFEKNN"
     gene            complement(8356..8613)
                     /locus_tag="KPD58_000626"
     CDS             complement(8356..8613)
                     /locus_tag="KPD58_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HBG0264413.1"
                     /translation="MEKDLDYTMKFEGIPEDRMSVGDTIDFVYKALVEKGYNPINQII
                     GYLLSGDSSYITSHKNARAIIKKFERDEILEEVITHYLNRK"
     gene            complement(8638..11277)
                     /gene="alaS"
                     /locus_tag="KPD58_000627"
     CDS             complement(8638..11277)
                     /gene="alaS"
                     /locus_tag="KPD58_000627"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBG0264414.1"
                     /translation="MEKMGLNEIRSKFLEFFESKGHYVANSYSLVPNNDKSLLLINSG
                     MAPLKNYFSGVEVPPSVRMCTSQKCIRTGDIENVGITARHATFFEMMGNFSFGDYFKR
                     ESIKWGWEFVTEWLNIPEDKIWVTVYEEDDDSYDIWAKEMNFPEERMVRLGKDDNFWE
                     IGTGPCGPCSEIYFDRGEEYGCDNPDCKPGCDCDRYLEFWNHVFTQFDRDEEGNYSLL
                     ENKNIDTGMGLERMGCIMQGVDTIFEVDTIKSILEAVEKLTGVKYGENPKNDISIRII
                     TDHIRAVTFLVSDGVLPSNEGRGYVLRRLLRRAARHGKLLGVKELFLQKLIDEVIKVN
                     DKAYPVLVEKESYIKKVVGIEEEKFNETIDQGTEILNSYIEVLKNEGKTVLSGQEAFK
                     LYDTYGFPIDLTKEILEEEHLSVDEEAFNEEMEKQKERARNARGNMDRESWKEDPLSK
                     LESTVDSTFNGYSEIYGEGTIEAIVKDDELVQSAEEGDKVSIVLDNTTFYPEGGGQVG
                     DCGLITNENLVLEVLNTKKGANNSIKHIGIIKSGRISNGDKVKTLVDRETRMSAARNH
                     SATHLLHKALREVLGEHVNQAGSLVTPERLRFDITHFEAISNEELKVIEEKVNNVILS
                     SLDIKCDIMNIKEAKEKGATALFGEKYGDEVRVVSMGDYSTELCGGTHLTNTSQVGMF
                     KILSEGGVAAGVRRIEAITGKAVYEYLKERDGIISEVCVNLKSKEDNLIQRISSLLEE
                     NKNLSKELHDMKAKMSLQSVDSIFDSKVEVNGVNLITNKFEGMDMDTLRETADNLRDK
                     LGSGVVVLANVVDDKVNFVVTATKDVLDKGIHSGNIVREVAKIAGGKGGGRPNMAQAG
                     ASDVSKVDQALSYASEVIKTQVK"
     misc_binding    complement(11345..11583)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(11726..12805)
                     /gene="mnmA"
                     /locus_tag="KPD58_000628"
     CDS             complement(11726..12805)
                     /gene="mnmA"
                     /locus_tag="KPD58_000628"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBG0264415.1"
                     /translation="MNKKVMIGMSGGVDSSVAAYLLKQQGYDVIGVTMKLWQDDDVVE
                     IEGGCCSLSAVEDARRVANKIGIPFYVLNFREVFKEKVIDYFIDEYLEGKTPNPCIAC
                     NKHIKFDDFYKKARQIGCDYVATGHYAKIEKDESTGRYLLKKSVTDKKDQTYALYNLT
                     QEQLEHTLLPIGDYEKDRVREIAKEMGMAVHNKPDSQEICFVKDNDYANYVKKHSKKR
                     IEEGFFVDTKGNILGKHKGILYYTIGQRKGLGITFGKPMFVIDINPINNTIVLGDNED
                     LFKKELIAKDVNFISIDTLEEPLRVQAKIRYSAKPSPATIHRVGEDTIKIVFDEAQRA
                     ITKGQSVVMYDGDIVVGGGIIEKSL"
     gene            complement(13094..13534)
                     /gene="nifU"
                     /locus_tag="KPD58_000629"
     CDS             complement(13094..13534)
                     /gene="nifU"
                     /locus_tag="KPD58_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HBG0264416.1"
                     /translation="MQYSDKVMEHFMNPRNMGEIDNASGVGEVGNPTCGDIMKIFLDI
                     DGDVIKDVKFKTFGCGSAIASSSMATEMIKGKTIKDALELTNKAVAEALDGLPPVKMH
                     CSVLAEQAVKAALIDYAQKNNIHIPELDGYVIDDAHDHDVEEEE"
     gene            complement(13588..14781)
                     /gene="nifS"
                     /locus_tag="KPD58_000630"
     CDS             complement(13588..14781)
                     /gene="nifS"
                     /locus_tag="KPD58_000630"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HBG0264417.1"
                     /translation="MEKRRLYMDYSATTPIKKEVLDAMMPYLTDYFGNASSFHTFGRE
                     AKDALDKAREQVAALINAEPSEIYFTAGGSESDNWTLEGVAYANKNKGNHIITSKIEH
                     HAILHTCEYLAKHHGFEITYLDVDSEGKVDLKQLEDSIKDTTILISIMFANNEIGTIQ
                     PIKEISEIAKKHKILFHTDAVQATGNIPVDVKELGIDLMSMSSHKIYGPKGVGALYIR
                     KGVRLHNFVHGGAQEKSKRAGTENIPAIVGYGKAAELAKENMQNHVETLTRLRNKLID
                     GVLERIPYTRVNGSLENRLPGNANFAFQFIEGEGILLLLDMLGIAGSSGSACTSGSLD
                     PSHVLLAIGLPHEIAHGSLRLTVGDFTTDDDIDYILENLPKVIERLRSMSPLYDDAKK
                     QGLVK"
     gene            complement(14799..15245)
                     /locus_tag="KPD58_000631"
     CDS             complement(14799..15245)
                     /locus_tag="KPD58_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBG0264418.1"
                     /translation="MKLSTKGRYGLKAMFELALNQDNGPVSLKFIAKKQKISDQYLEQ
                     IFSSLKKSGLVKSVRGAQGGYLLSKNAEDITVGDILVVLEGPVALSDCVLDEDVCENS
                     NMCVTKIVWEKMKKGIEDVIDSITLKDMINDYNKNKLENDITNIKK"
     gene            complement(15354..16124)
                     /locus_tag="KPD58_000632"
     CDS             complement(15354..16124)
                     /locus_tag="KPD58_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0264419.1"
                     /translation="MIIKLNSTYHSRVMKYLKKEPEYNLFIIGDIERYGYGNNFLNIW
                     ADVGEHGEIKAILLKYFEFMMFYSDGEYDVEGFYNLLRNTNYEEISGKICAVDALAKR
                     LGLNNLKVVDFCKLQTKKFLIDNNCNAKVKRIRLGNLKKTVKLYDLIDEFHSTTLENL
                     KNGLRTGRGYCIEINKQVVSMAKSTSENRTHAMIIGVGTHPKYRAKGLATKCLIKLCS
                     ELLRENKIPCLFYDNEEAGKIYKKLGFENIGKWGIYSK"
     gene            complement(16155..17420)
                     /locus_tag="KPD58_000633"
     CDS             complement(16155..17420)
                     /locus_tag="KPD58_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HBG0264420.1"
                     /translation="MPLADKIRPKTMNDVIGQSHIIGNGKILSKILQSNFLPNMIFFG
                     PPGVGKTTVAEIIAERSNKSFYKINATNSSLEDIKKVISELGSINNVNGVLLYIDEIQ
                     SFNKKQQQSILEFMENGSITLIASTTENPYHYVYKALLSRSTVFEFKPLEKLDIEKGL
                     KRAVEVLNEDSYMDIECNNNAIEDIAILSDGDMRRALNILEVVVYSTKPNKDNITYID
                     SDVIRASTFNKIINYDKNGDSHYDILSAFQKSIRGSDPQASIHYLARLIKGGDLISIC
                     RRLLVIASEDIGLAYPNAIVIVKACVDSAMQLGFPEAKIPLAEATILLATSPKSNSAC
                     IAIMKAMDELDKEFVKDIPNSIKDAHYSGSKEIGRGCGYKYPHNFKNHYVKQQYLPDS
                     IKDSIYYIPQENKTEKNIKKYLEYLNSDF"
     gene            complement(17527..18312)
                     /gene="codY"
                     /locus_tag="KPD58_000634"
     CDS             complement(17527..18312)
                     /gene="codY"
                     /locus_tag="KPD58_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HBG0264421.1"
                     /translation="MASEVLQKTRKINKTLQTSGGSSVSFDLLAGALGDVLSSNVYVV
                     SAKGKVLGLHLNDVQDSSVIEDEYTKQKKFSDEYTQNVLKIDETLENLNGEKILEIFP
                     EEHGRLQKYTTVVPILGSGQRLGTLVLSRYSNSFNDDDLVIAEYSATVVGLEILRAIG
                     EELEEEMRKKAVVQMAIGTLSYSELEAVEHIFAELDGKEGLLVASKIADRVGITRSVI
                     VNALRKFESAGVIESRSLGMKGTHIRILNDKLTDELKKLKNNQ"
     gene            complement(18477..20564)
                     /gene="topA"
                     /locus_tag="KPD58_000635"
     CDS             complement(18477..20564)
                     /gene="topA"
                     /locus_tag="KPD58_000635"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBG0264422.1"
                     /translation="MAKTLVIVESPAKAKTIEKFLGKSHYTVKASVGHVRDLPKSKLG
                     VDIENNFEPQYINIRGKGDVIKELKKEAKKSKQVYLATDPDREGEAISWHLAHILNLD
                     ESEDCRIEFNEITKDAIKKAIKHPRNIDINLVDAQQARRVLDRLLGYQISPILWQKVR
                     KGLSAGRVQSVTTKLICDREKEIKAFIPKEYWTIDVEAKTVKDKDITLKFYGKNDEKI
                     ELENEEAVNQILKEMEGKDLKVEKIESKSRRKSAPKPFTTSMLQQEGANKLFFTTKKT
                     MMIAQELYEGIDIEGEGTVGLISYIRTDSKRISDEAKEKAKGYIVNNLGENYYKNTGD
                     KKEKKDTKKVQDAHEAIRPTSVDRTPESIKASLSKDQYKLYNLIWRRFVASQMEDSVF
                     DILNVECKIGDIVFKATGSKMKFDGYTKVYNFSEREDKILPSIEKGDILNIENILPDQ
                     HFTQPPARYTEASLVKTLEELGIGRPSTYAPTIATILNREYVEKQGTSLCPTELGIIV
                     TDILENNFHKFIDVDFTADMESKLDVIEEGNTDWKKIVSESYAPLKEAIEEALENIEK
                     VNMDEETDEICENCGSNMVIKYGRFGKFMACKNYPDCKTTRPIMNKIGVKCPKCEEGD
                     IILRKSKRGKAFYGCSNYPNCDFVAWNKPTGEFCEKCGSYMVEKVTKSETKTVCSNKE
                     CNKETKEHNDGKE"
     gene            complement(20597..21697)
                     /gene="dprA"
                     /locus_tag="KPD58_000636"
     CDS             complement(20597..21697)
                     /gene="dprA"
                     /locus_tag="KPD58_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBG0264423.1"
                     /translation="MEKRDTYLWLKSIGGITTKTIEIIENEIVNIEDIFDFSEKEIYN
                     LKNISLNIRKNIVKYRGHAYLENIKELLYKKAIKYICKYDKEYPENLKNIYNAPKLLF
                     YKGDIGLVNNNFNIAIVGSRKPTAYGINCAKTISCQLSQYGVNIVSGLAIGIDAYSHI
                     GCMSGKSKTIAVLGSGVDNPLPKQNLHLSNKILENGGLLLSEYNINSTVAPYHFSNRN
                     RIISGLSDGVVVVEAAIKSGALITVDFALEHGKNVFAIPGNINSQMSRGCHKIIKEGA
                     KLIENIDDILNEYNIFNIIDKKINQKYDNISLNAKSKQIIEAIKREGNLHIDSICDYT
                     GIEIKYVNSIINELVLNELVVEMNNKTYSLNV"
     gene            complement(21716..23317)
                     /locus_tag="KPD58_000637"
     CDS             complement(21716..23317)
                     /locus_tag="KPD58_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HBG0264424.1"
                     /translation="MLSIINSSNLVGIDSFLVKVEVDVSNGIPSFNIVGLPGKEIKEA
                     RERVKSAILNSGYKFPSTRIVVNLSPADIKKEGAFLDLSISIGLLRELIKKDENYIRE
                     SMFIGELSLDGKIRKVRGILPIIMGAKTQNIKRIFIPIENIKESLFVDEIDIIPIKSL
                     KECVDFLNEEIKVDKVRIMSFLDDKSRKENEELEKDNSYIDCKYTKINNEESKYDEDF
                     KDVKGNYFVKRSAEIAAAGNHNMFMIGPPGSGKTMIAKRVRTILPDISVEEMIEVSKV
                     YSILGMINETKGIIDKRPFRAPHHTTTKQSLIGGGMDARPGEIALAHRGILFLDEIAE
                     FDRKILETLRQPIEDGYVNISRVKYSAKYPCRVLLVAAMNPCPCGYYMSDTECRCRSN
                     EIDRYINKISGPLLDRFDIFVEVNSIKYSDFNSLKQEESSQKIKRRVENARKIQINRF
                     KKDNIKNNSEIKAYNLFKYCKLEKEASKTAEMIFNKYNLSSRSYTKLLKMARTIADLE
                     ERDLINSQCIIEAFSFRKAYYSYFK"
     gene            complement(23348..23692)
                     /locus_tag="KPD58_000638"
     CDS             complement(23348..23692)
                     /locus_tag="KPD58_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HBG0264425.1"
                     /translation="MNNKEKGDFGEKVAVNYLLSKGAKILEKNYRLKIGEIDIIAKME
                     DEIIFVEVKSRSNIKFGYPCESVSFKKRKKIIGVASYYIIKNNLNNTPIRFDVIEVYL
                     LEKRINHIMNAF"
     gene            23876..25039
                     /locus_tag="KPD58_000639"
     CDS             23876..25039
                     /locus_tag="KPD58_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG0264426.1"
                     /translation="MNREKLVAFIRYLSLFILVVSYLKNVENTLYPVITMIILFLIII
                     NNQVRFFSLSNENTFILISYFLELILIFLLCNYTKTFNSIYFVPLILDISFLIKEKYK
                     YILFSFVIISSLVISLDKNIYLALESSSILLIITVLSIYIENENLSKLYSQNLYDKLR
                     ISKDELKKVNADLETYASSIEELAILKERNRISREIHDSVGHSLSTTIIQLNAIEKLL
                     KDKPLIYDLVHELREFVTESFQDVRRAISELKPVEYENYQSLFKIKELVKSFIKLTNI
                     NVKLTISKNTWNLSRNQSITLYRLIQESLSNSSRHGKATEIRIFITFNTSNLIITISD
                     NGIGCGNIKKGNGLNSINERIYELNGKVEFDNSNNGFTIRASFPKFSGGDFFE"
     gene            25032..25679
                     /locus_tag="KPD58_000640"
     CDS             25032..25679
                     /locus_tag="KPD58_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0264427.1"
                     /translation="MNKIKVLIVDDEKLIRKGLKIILSSYNDLEIVGDASNGYEALEF
                     CKMNDVDIVLMDIRMKVCDGVLGTRLIKEYNNSITLLILTTFNDDEYIKDAMKFGASG
                     YLLKDSSDEVLHEGIRSSFFGNIVLDKSVAEKIMTSEKTVKQEHLYDMYNLTEKEISI
                     IRLIANGLNNKEISQELFLSEGTIKNNITNILAKLELRDRTQLAIFAFKNKIVIE"
     gene            25808..26743
                     /locus_tag="KPD58_000641"
     CDS             25808..26743
                     /locus_tag="KPD58_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0264428.1"
                     /translation="MDIVRVNNITKRFNDKLVLDNISFSVKKGEIFGLIGPNGAGKST
                     LINIITNLMLPNSGSIQINGLDLSKDYIKAKSIIGLVPQELAIIETLTSFDNLEYFGA
                     FYGLKGKLLKERIIEALEVTGLTEVKKKKVKKLSGGMQRRLNIGIALLNHPKILILDE
                     PTVGVDPQSRNHIFNFIKDISKKHETTVIYTSHYMEEVEHLCSKIFIMDEGKEIAFGN
                     NDYLKSLVSTNTKLIMEIKNINAQLIFDLKNTKGVISVLENNSLLELDIDKKLQLTDI
                     LSVIDKNNSKIMKISYEEPSLEDVFLNLTGKNLRD"
     gene            26757..27854
                     /locus_tag="KPD58_000642"
     CDS             26757..27854
                     /locus_tag="KPD58_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0264429.1"
                     /translation="MKLYMSVKTMLKSLKSSFILNLIYFLALPLILSWFLGMVTESMF
                     QNPIKTESTPIVIYDKDNTRLSNDLTKYLKNDLSYILTVKKDDSKAELKLTIPKGYES
                     SLLNEKSNTLNIEKLGTRDDIAILLQDILDTYHEKFYLNNSQKISSEDFSKLFNKNSI
                     DTSIINNNVKQSSYEYFALVSLGFLVIIFIMNNILSNYISESKGLSKRLYSMPITRVQ
                     FLIYDFVGLWIYSFIFLLLYVLFFRILGITFKGNFAILLLLCALSSYFMTSISTFVTS
                     FFSKKYGTAIVYALLFLQTIFGGIFSMISDTFTKLTSLSPTYLIGELFSNYETFKTID
                     SIGDLIFTCLIISTILIALAIVKENYKWREV"
     gene            27857..28921
                     /locus_tag="KPD58_000643"
     CDS             27857..28921
                     /locus_tag="KPD58_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0264430.1"
                     /translation="MIRLTIANIKRYLKNHTLLINMVMLPIILIFSLNFFINNSGNQS
                     MYFSPVAIVSDSSGKYEHKLINSSKLKENSFRLNEQDKAMNLLKNNKVSAVFVLDKNF
                     SNSIDKLQRPVVKCFKIENGGGSLWAESQIESFITKSLKLKVDKNIDDKLTKTNIIDN
                     KPDKNKGSFLVVFLICYFMYINAAHLASDLFTLKKSNVLKRLISTDNKDIKIIFSIFL
                     GLFFIQSIVYTFALLCFSIIEDFNLSLNVLMIVLANSFVSTGFVFWVARVFKNESSIS
                     LISTFYSLIGLAISISSLIPSMDKLSFMTNLSKFTPFYWTIDAIKNNGSILINIIALI
                     LIGIIFVTAGSLKLRDFAKN"
     gene            29165..29662
                     /locus_tag="KPD58_000644"
     CDS             29165..29662
                     /locus_tag="KPD58_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HBG0264431.1"
                     /translation="MYKIGESVMYPKEGACSVNDIVTKKINHEMQKYYELSVIFNSNL
                     KISIPVLNADRIGIRPVMDGNDVDNFIQSINKTDGVWIFDRKERLKLYQDKFHSGDVF
                     EIVKLIKMLMIQDSSKQLCSTDKEFLNKAQKFALSELAAAQCKSYTMVLEEMKKHILN
                     SKNIN"
     gene            complement(29839..30087)
                     /locus_tag="KPD58_000645"
     CDS             complement(29839..30087)
                     /locus_tag="KPD58_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264432.1"
                     /translation="MSRKAYEEALVELEKFIDERKEIIKSAEDCIDKYIVDRTLPFDY
                     KDKCVEWQQELLDIAEAQVLEANELSVLLQEKKELEED"
     gene            complement(30153..31169)
                     /locus_tag="KPD58_000646"
     CDS             complement(30153..31169)
                     /locus_tag="KPD58_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBG0264433.1"
                     /translation="MEYNELLKSARENLNGSCKVCKVCNGVACAGEVPGMGGKGSGSS
                     FIENSKSLEKVKVNMRVIHDVSNPDTSIEMFGKKMSAPIFAAPVTGTTLNMGGKINER
                     DYIEPVVAGCANSGIYAMVGDTAVDAFLMENLDVVKKYNGAGIVFIKPWDNENIIKKI
                     RLAEEAGAFAVGVDIDACGLVTLSLHGKPVLPKNVKQIKELVKSTKLPFILKGIMTVE
                     DALMAVEAGVDAIVVSNHGGRVLDCTPGACEVLPKIADAVKGKVTILADGGVRTGLDV
                     LKMIGLGADAVLIGRPFVTASFGGATDGVETYVNKLQSELSSSMILTGCQTIKDIDGK
                     VIYK"
     gene            complement(31227..31994)
                     /locus_tag="KPD58_000647"
     CDS             complement(31227..31994)
                     /locus_tag="KPD58_000647"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBG0264434.1"
                     /translation="MQDKSVREIKEIIETLEVEKYMEYIELLRVDERKSVQGLAIKLA
                     KKLDNIRKEEERLETINIFENEGYDKGYLYIGGIDEAGRGPLAGPVVASVVVFKKDTK
                     IEGVNDSKKLSEAKRDELFEVIKEEALDYGIGIVNNEEIDEFNILNATYMAMKKAINC
                     LKKAPDYLLVDAATIPGIDISQNPIVKGDSKSISIAAASILAKVTRDSIMYQYDRVYP
                     EYGFKSHKGYGTKEHYEAIEKYGITPIHRKSFLKNIL"
     gene            complement(32062..34251)
                     /locus_tag="KPD58_000648"
     CDS             complement(32062..34251)
                     /locus_tag="KPD58_000648"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HBG0264435.1"
                     /translation="MKYIIKRDGTKELFSKSKIEDAVYKASINSEGGVDRELAYDISR
                     KIANDYNSMEKCLSVEEIQDIVEVLLMESNRKDIAKHYILYRERRSEIRTKAWEFDEL
                     QKSIWKNKYRHNGESFDEWIKRISNNSSRISKLIRQKRFLFAGRILANRGLQKEGKKV
                     TYSNCYVIEPPKDSIEDIFDTAKKLARTFSYGGGVGIDISKLRPKGAKVNNAAKHTTG
                     AVSFMELYSMTTGLIGQRGRRGALMISMDVNHPEIEDFIDIKTDLEKVTKANISVRVT
                     DEFMQAVREKKLYNCTFEVETTGEKIVKIVNAYDLFNKFVKNNWDYAEPGILFWDKIK
                     TYNFMSEDKNFCFSGVNPCAEEPLPSGGSCLLGSINLSEFIIQPFGEHATFDINKLKS
                     CVREAVVALNDVLDEGLNLHPLQEQRDSVHKYRQIGLGVMGIADMLIKMGIRYGSDES
                     LELCDLIGKTMLNEAVKQSALIAKEYGTFSEYKYENISKSKFFIENLNEDTKEMVKKY
                     GLRNSQLLTIPPTGSISTMIGISGGIEPIFNLSYIRKTESLHDEDVYYKVYTPIVKEY
                     MDKKGITDEECLPDFFVTAMNIGYEERINMQKVWQKYIDASISSTINIPFETTVEDVF
                     NIYIKAWEAGLKGVTIFRDGCKRSGILINEKKEENNKEDKKENSKKEYYKNNKKEKDN
                     KDEKAKEIINKNFEEDEKFVCPECGSEGIAHTGGCSICLKCGYSGCN"
BASE COUNT        10833 a         6031 c         3685 g        13797 t
ORIGIN      
        1 agggtatttt ttatgtgtat tataatcata tctttgtgta ttataattat atttcatctg
       61 ataattattt atatcatatt atcaattatt ctttgaagca tttgtagata gcttgcctta
      121 tttatatcct tagtatttat caattccact tcgcctatta cttttttatc tttacatatc
      181 tttatttttc ctaaaacagt tcccttttta attggcatct taggattttt tactatttca
      241 attttcttct caaattcttt agaacttcct tttttaataa gagcatttaa atcttcttta
      301 gcaaccaatt taactttgtt ttcatcagcc ttatccaatg taagtgttgc tatattatca
      361 cctttactac ataacttaac actttcataa ttagcaaatc catagtttaa aaggctagag
      421 gcatcattaa atctttctgg agaagtctct gctcctaaag ttgcagcaac tagatgagta
      481 ttacctctct ttgcagatgc tgataaacag tattttgctt cttgagtaaa accagtttta
      541 acaccagttg ctccttgata atgctttata agtttattag tatttgcaag acctactgta
      601 acttgtttct tccctactac aaccttatcc atccatgtag ttaagtattt acttatcatt
      661 tcgtgtttta atagttctct agacattaaa gctatatcat gagcagatgt atagtggttt
      721 gcaactggaa gtccatttgt atttgcaaaa tgagtatcat tcatatttaa ctcttttgct
      781 ttagcattca ttctatcaac aaatccttca acacttccag ctaagtgttc agccatagca
      841 acacaagcat catttgcaga agcaacagct atacctttta ataatgtatc aaccttttga
      901 gtttctcctg gctctaggaa tatttgacta ccacccatac tagatgcagt ttcacttact
      961 tgaacttcat catcaagctt aatcttgcct gaatccaaag cttctactat aagtagcata
     1021 gtcattacct tagttacact agcaggagga agctttttat gagcatcctt ttcatataaa
     1081 atttgtccac taccaacatc catcaaaatt gctgattttg aagatatatt tagttggttg
     1141 gcatcagcat tttccttatt atttgcaaaa gaaaagctta aaggagtaag cgcaattaat
     1201 atagccaaag cagtactagc taattttttc atcgatgtca cctcttaaag tttatttaaa
     1261 cttattttct cacatttaac tttttttatt ctaaatgcct agatatacct tgacctaaat
     1321 tttttttctt ttcaagcttt ttaaagtaac cttctaaaac acctttatta gacgcatttt
     1381 ctattatttc aaaagcatct tcataattca ttccatactc tcctgctatt tccatttttg
     1441 tttgttttaa agcttttata gcattttgtc ttgaaacatc atccataaga atcctcctat
     1501 cagtaaaatt tatatataag tagtttttac taataaaaga ttgttataat tggtatttta
     1561 tgtaatatat atttttttaa aatgagtatt aaaaagacct cttgtaattc ttaagataat
     1621 atcaattgga tttttgatat tactttaaaa attacaagag gtctaatttt caaatttata
     1681 gtttttttat attatacttc cattatgata ggaagtatca ttggatttcg tttagttttt
     1741 tgatataaat attcttttag gttttcttta atattattct ttaaataagc ccattcttta
     1801 atatttttct cttcacattc atttagaaca ttcttaatta tatcctttgc tccatccatt
     1861 aaatcttctg actctcttac atatacgaat cctctggaaa ttatatctgg acctgctaag
     1921 accttaccct catccttgga aatagtaact acaactatca taagaccatc ttctgataaa
     1981 tgctttctat cccttaaaac tatattacct acatcgccta ctcctagacc atcaactaat
     2041 atgtttcctg tttgtatacg gtttgctacc ttagcagaat ttttatctaa ttctaaaaca
     2101 tctcctgttt gcatgacaaa aatattttct ttatccatac caagctgttc agctatttca
     2161 gcatgtcttt ttaacattct atattcacca tgagctggca taaaaaactt tggtctaact
     2221 aatgcatgta taagttttaa ttcttcttga caggcatgtc cagaaacgtg tatatcagct
     2281 atatcactgt atactacttc tgctcctttt tcaaataaga aattgattac tcttgatatt
     2341 aatttttcat ttcctggtat tggatgagca gatattataa ctaaatcgcc actctttatt
     2401 tctacttttt tgtgctctga aaaagccatt ctagcaagag ctgacatagg ttcaccttgt
     2461 gaaccagttg ttattatcac taattcacta tcttcatact tacatatatc atttaaatct
     2521 ataagcatgt catcagaaat atctaggtat cctaattctt ttgcaactcc aactacattg
     2581 accattgagc gacctgatat agcaactttt ctattaaact tttcagcagt atttattatt
     2641 tgttgtaatc tgtgtatgtt ggatgcaaag gtagcaacta taattcttct gccatttcct
     2701 tttgcaaata attcgtcaag acctacccca acagtctttt cagacatagt aaatcctggt
     2761 ttttctgcat tagtactatc tgccagcatt aaaagaactc cttttttgct caactcacag
     2821 attctatgca tatccataac atctccatca ataggagtca aatcaatctt aaaatctcct
     2881 gtgtggtata ttataccttg gtctgtgtgt attgcaatag agtaagcatc tggtatacta
     2941 tggttatttt tcaaaaactc tacttccata ttatcaagct ttataacttg tctaggacct
     3001 ataacattta acttaacatt attcatttta tgttctttaa gttttacttg tataagacct
     3061 atactaagtc ttgccccata tacaggtaca tttattttct ttaatatata tggaagagct
     3121 cctatatggt cttcatgtcc atgtgtaata aatataccct ttattttatc cctatttttt
     3181 actaaataag ttatatctgg tataactata tctactccta acatttcatc ctctggaaaa
     3241 ctaagtccag catctataac aattatttca tctttatact caacaacagt catatttttt
     3301 ccaacttcgt taagtccacc caaggccatt acctttatct tgttggtgtt ctttttgaac
     3361 aattgcatca tctccttctg tctatttaat tttttaacac gttctacaat cataaataat
     3421 atcaaatcac gcactcgcta ccagttattc ttattgtacc ataattcgta tcatatttta
     3481 agctatattt aaaatacatt taacgtatta ttttgacaaa cctattttat tttgatatcc
     3541 tctaaatatt atatgattta atatcggata atttaggagg atttatgcgt ggtaaaactc
     3601 attgtgcaat aggaatctta acagctatcc aaacatcttt aatatttaaa attcctatat
     3661 ctttagttga tattttagtt tcagctactt ttgctgtact acccgatttg gataaatcta
     3721 attctatggt ctcaaatttc attctaaaaa acaatgtatc caaatacata tatagaattt
     3781 ttatatatgc tgttaatatt attattttct ttatttcaat aaatataaat gataattttt
     3841 atttaagcgc aataattaca tttgttgcaa ttataattat tgaagcaaaa ctaacccaca
     3901 catttctaag aaaagttttt ctatcattaa tatttatact attagctata tgcttataca
     3961 tcatagaagt tgaaatatat tttacaattt tttgtctaat gttgtcaatt tttccctggt
     4021 taaagcacag aagtttttct catagcatat ttgcaactat aatagtatat tttttactaa
     4081 aacagataga attaatcact aatattaata atttatcttt ttatggtacg attggttatg
     4141 caagtcatat gtttttagga gatttattta caaagcaagg aataccaatt ttttatccgc
     4201 taagcgaaaa gaaaatttct ttaggttttt taactgttgg aggaccattt agtaatttca
     4261 tagaaaaatc attcattttt gtacttatag gattaattat attttcaata ttaaaactat
     4321 aagcatcttg aaaactgtat atttgcattt tgtagatggt ttataataaa aaatggaaga
     4381 atagcataga atataaaagt tctatactat tcttccatcg acattaagca atagaagttt
     4441 attcttctga atcgcttact ccattacact cgtcacatag tccataaaat ttaacatcat
     4501 gatcaagtat tttaaatttg tacaatgatt gtatctcttt ttctatattg tctaaaaggt
     4561 cttctttagc ttccattatc tttccacaat ttttacaaat aagatgatga tgattatgac
     4621 agtcttcttt atgtagacta atctcatatc ttatacatcc atcgtctaag tttagttttg
     4681 atattaatcc tatttcatcc aatagctgca ttgttctata tacagtagct aagcctattt
     4741 caggacagtc aaccctaact aaatcataga tttcctcact acttagatgt gaatggtcat
     4801 gtttaagcaa tatttcaact attgctcttc tttgtggcgt aatcttaaaa ccagtttctt
     4861 ttaacttgtc ttttaataaa tccattgtat ttgccataac atcaccctta ctattttcaa
     4921 ttgataatct ttattaaata atattgtaat tgataattca ttttttgtca agacatttaa
     4981 ataaatttaa tttattctca taatataaat atatacccat aaaagatata ttattcacca
     5041 aaattcataa attttttggt atttttaata aaaaacaaaa aaacagatat atactataaa
     5101 tgcttttaca attaataagg tttaattaaa gcaaatataa taaatatctg atttttatat
     5161 acaaacttaa ttacttatct agttaagtcc atttgattca tcttcgtcgt aatttaaacc
     5221 ttcttcttcc attatagcag tatacacagc tacaacagtt tcatactctt catcactttc
     5281 caaaggtatt aagatttcac cttcttcatc ctcatcaatt ctaaagatta aagcttcttc
     5341 agcttcttca tcatctaaag gcattaatat tgcatactct tttccttctg cttctaatgt
     5401 taatattatt tcaaattggc tttcaacgcc attttcgtct attaaattta ttatattttc
     5461 ttccataatg tcattcacct caaatttatt tttattttta ttatttatat tatatatcat
     5521 aagtattcaa ttttatagtt cttttatgaa tttattttaa aatctaaata tccttgcaaa
     5581 attattactg ctgccaacat atctataacc ttttttcttt tttgtctact aacatctgct
     5641 tctaataaag acctctctgc cgctactgta gaaagcctct catcccaaaa ctctacatca
     5701 atacctgttt ctgcttttac caattcacaa aacttgatta ctttttctcc ttgtggacct
     5761 aaagttccat tcatattttt tggtaatcct gatacaattt tatttacttg tttttctttt
     5821 attattgctt ttagctcttc aatatctttt ttcttgccaa ctctttttat tgtttttaca
     5881 ccttgtgcag ttaatcccat caaatcactc acagcaactc caatagtttt atcaccaaca
     5941 tcaagaccca ttattcttcc atctaacata gttcctcctt tatctattta tattctatag
     6001 attaaataca tattctgaac ctctcataac taaggtcaca ggcttactgc ttcatataat
     6061 tttacataat caatcataaa aattgcaata tgatttttta tgtaagcatc cagtttacat
     6121 taataatcat attgcatttt ttatttaaat cctatatgaa cagtaaaaat ttttttagtt
     6181 atttttttca aatattatca tatttatact tattattatt agtggtataa gtatatatat
     6241 agatgctgta gatgcagcaa ctattccaga atcattaact aaaagagtaa ttatacaacc
     6301 aaccatagat gcaataaacc ctttaaacaa gattggaaaa ttattcatta tctttctaaa
     6361 gtgtctagat ggtttaaata taagcactgc aattactcca attcctacaa gtaaaatatt
     6421 tacccataca cttgtctgtg ctagttgcat attcatttga atttttcttc caaatgtttg
     6481 taatatctct cctggtccat ttaggaaaat tctctgtaca aacataccta aatgagattc
     6541 actacctgaa agtatatcta aagctgcaaa tgcaaaaacg acaactacag ctgaaacagc
     6601 taataataca gctttcttaa aatctatttt aatgtcatat ataagcatta taaacaacaa
     6661 atatgctaca cattctgata tagctccacc aacatttgca cccattgaag gatatgcact
     6721 agttataagt attattaatg aaaaaattac aacaagtaat ttagatacct tcttataatg
     6781 taataatact gctaagccaa atactgcact tgctatagtt acaccttcat attcatttcc
     6841 tacaccatag tacctagctc ctatgacagc atcataactc attatgttat tttgcattaa
     6901 gtatgttcca aatacagaat ctataactat gactaaaata gtaagtgttg caaaaaatcc
     6961 catgtgcttt atatcattgt ccttaaataa ttttctacct aacaagtata atattagcgt
     7021 agtcgcaaat attcccaaag atattgaaat tggagtttta aaattcatca aaggagctaa
     7081 cataaatgat agtggcatta ttatgcctaa ttttataaac tccttaagta ctttgaatac
     7141 acgctcttta tgtggcatat aatttctaaa gaaaatcgca aacatggcaa ttacccaaga
     7201 agcagatatt acgcctacaa agccatttat aatagttgac cttattgaag atatagacac
     7261 tatcctttca taaagtgatt ttagatagct aatattgtcc tctttattta ctaaagtata
     7321 acttctacct accatctctt tattttcaag accaaatgta cctagtatat ctactccaac
     7381 atctaaattg gcaacaattc cagcctttct tgttgtggca gattccagta gacctttccc
     7441 ttctccacta aattttatta ttggagaaag tcttctctta ttcttataat ctaggtcact
     7501 tggaaatgca cttgctatat aaacagtgtc attttctcca accatagaaa atacttgttc
     7561 aagatattta tttatatttt ttacaatatt tttcttcatt gtatcataag tattatcatt
     7621 tagatttaat ctatacatat ctaatctata tgtatctcca agttctacaa atatagcatc
     7681 actattttta taggattttt tagtttcatc tagtaattta tcataatctg tagatattcc
     7741 aaatggcatt ttagaatctt tcttattaat attatcaata ttacctgaat caatccttcc
     7801 atattggtcc atagctacta aacataagtt tctgttttta actaattgat tattttcaat
     7861 tatatcagca tttccaatta cagatgtctt taaaccattt tctgataaag tttttcctaa
     7921 tgaccctaaa tttgctccat attctccata ctcaagattt tcatttatag atctattaat
     7981 gcctaaatca tttatttctt tagctttttg acctgttgcg acttcaaaag ctttagcagt
     8041 atcttggtct actttttgaa aattgatata gtcttcagta gttacactag ctcttgttcc
     8101 tgcacccata cttgcataag accttctatc atcagtacct tgatcccctc ttatattcat
     8161 gagagctaca tagcctctct tttctatctc atttttcaag attggaatat tcatcatact
     8221 tccaagattg gtcctattca tatcaataaa tatgaccttg cctttttctt cagcaaaacc
     8281 atagctagca gttgatacaa aaatcataat gactattgca aacaacgaca taatcttttt
     8341 tctcatttat ttgacctact ttctattcag atagtgcgtg attacttctt caagtatctc
     8401 atctctttca aattttttaa ttatagctct tgcattttta tgactagtta tatagctaga
     8461 atcaccagaa aggagatacc ctataatttg atttataggg ttatatcctt tttcaactaa
     8521 tgctttatat acaaaatcaa tagtatctcc tacactcatc ctatcttctg gtattccttc
     8581 aaatttcata gtgtagtcta aatctttttc caaactaaaa acctccttga gtgaatgtta
     8641 tttaacttgt gtcttaataa cctcacttgc atagcttaaa gcttggtcaa ccttagatac
     8701 atcactagct cctgcttgtg ccatattagg tcttcctcca ccttttcctc cagctatttt
     8761 tgcaacttct ctaacaatat tacctgaatg tattccctta tctaatacat ctttagtggc
     8821 agtaactaca aagttaactt tatcatctac tacatttgca agtactacta caccagaacc
     8881 aagcttatct cttagattat ctgctgtttc tcttagtgta tccatatcca taccttcaaa
     8941 tttattagtt atcaaattaa ctccatttac ttcaacttta gaatcaaaaa tagaatcaac
     9001 tgattgtaaa ctcatcttag ctttcatatc atgcaattct ttagataagt ttttattttc
     9061 ttctaataaa gaacttattc tttgaattaa attatcttct ttagatttta agtttacaca
     9121 aacttcagag ataattccat ctctttcttt taggtattca taaacagctt ttcctgttat
     9181 tgcttctatt cttcttacac ctgctgctac tccaccttca gataatattt tgaacattcc
     9241 tacttgagaa gtatttgtca agtgagttcc tccacaaagt tctgtagaat aatctcccat
     9301 agaaactact cttacctcat caccatattt ttcaccaaat agtgctgtag ctcctttttc
     9361 tttagcttcc tttatattca taatatcaca ttttatgtct aaagatgata aaataacatt
     9421 atttactttt tcttctatta cttttaattc ttcatttgat atcgcttcaa agtgagtaat
     9481 atcaaatcta agtctttctg gagttactaa agaaccagct tgattaacat gttctcctaa
     9541 tacttctctt aatgccttat gaagtaaatg tgtagcactg tgatttcttg cagctgacat
     9601 tctagtttct ctatctacga gtgtctttac tttgtcccca ttacttattc ttcctgattt
     9661 tattatacct atatgtttta tactattatt agcacctttt ttagtattta atacttctaa
     9721 aactaagttt tcattagtta tcaatccaca atctccaact tgtcctccac cttcaggata
     9781 gaaagtagtg ttatctagta ctatagatac cttatcgcct tcttctgcac tttgaactag
     9841 ctcatcatct ttaactatag cttcaatagt tccttcacca taaatttcac tatacccatt
     9901 aaatgtactg tcaactgtag attctaattt tgatagaggg tcttccttcc aactttctct
     9961 atccatattt cctctagcat ttctagctct ttctttttgc ttttccattt cttcattaaa
    10021 agcttcttca tctacagata gatgttcttc ttctaatatt tctttagtta agtctatagg
    10081 gaagccataa gtatcatata gtttaaaagc ttcttgacca cttaggacag tctttccttc
    10141 attttttaat acttctatat aagagttcaa tatttctgtt ccttggtcta tagtttcatt
    10201 gaatttttct tcttctattc caactacttt tttaatataa ctttcttttt caactaaaac
    10261 tggatatgct ttatcattta cttttataac ttcatctatt agtttctgta aaaataattc
    10321 ttttactcct aataacttac catgacgagc agcacgtcta aggagtcttc taagaacata
    10381 tcctcttcct tcatttgatg gaagcactcc atcactaact aagaatgtta cagctcttat
    10441 gtggtcagtt attattctta ttgatatatc attttttgga ttctctccat attttactcc
    10501 tgttaacttc tcaactgctt ctagtataga tttaattgta tcaacttcaa agatagtgtc
    10561 tacgccttgc ataatacatc ccattctttc aagacccata cctgtatcta tattcttgtt
    10621 ttctaataga ctataatttc cttcttcatc tctatcaaat tgagtaaata catggttcca
    10681 aaattctaaa tacctatcac aatcacagcc tggtttacag tctggattgt cacatccata
    10741 ttcttctcct ctatcaaaat atatttctga acaaggacca caaggacctg tacctatttc
    10801 ccagaaatta tcatcttttc caagtctaac cattctttct tctgggaaat tcatttcttt
    10861 tgcccatata tcataagaat catcatcttc ttcatataca gtcacccata tcttatcttc
    10921 tggtatattt agccattctg taacaaattc ccatccccat ttgattgact ctcttttaaa
    10981 gtaatctcca aatgagaagt tacccatcat ttcaaagaat gtagcatgtc ttgcagttat
    11041 acctacattt tctatatctc ctgttcttat acatttttga gatgtacaca ttctcacact
    11101 tggaggtact tctacacctg aaaagtagtt tttcaaaggt gccatacctg agtttataag
    11161 taacaaactc ttatcattgt ttggtactaa agaataacta tttgctacat aatgaccttt
    11221 tgactcaaaa aattctaaaa acttacttct tatttcattt aatcccatct tttccatatc
    11281 atgttcctcc taataaattt tattttttcg tttaaataaa aaacctcgtc cctataaata
    11341 aatttatagg ggcgaggaca atcgcggtac caccctaatt atcatacaaa gtatgatatc
    11401 ttcggaaaac cttagctata acgtaactac cgtcaatctc tacttaaatc ttctttataa
    11461 aatataaatt gttcaagatt gctactaaaa ggctgcttca aatatcatat tctaagggct
    11521 tccagcattt ccctctctct gtaagacatc aaatatttta ctattcctta tcactgtatt
    11581 tgttttttaa ttaatatact aattatatta ctaaatttta caaaattaat caatactata
    11641 ttttcatata caataaattt attttataaa ctttatttac tcttaatata ctttattgct
    11701 tattttttta attttttaga tgtttttata aacttttctc gattatacct ccacctacta
    11761 caatatctcc atcatacatt actacagatt gacctttagt aatagctctt tgagcttcat
    11821 caaaaactat tttaatagta tcttctccta ctctatgaat agtagctggt gatggttttg
    11881 ctgaatatct tattttggct tgaactctta agggttcttc taacgtatct attgatataa
    11941 agttaacatc tttagcaata agttcctttt taaataaatc ttcattgtct ccaagaacta
    12001 tggtattatt tattggattt atgtctatta caaacattgg ttttccaaaa gtaattccaa
    12061 gcccttttct ttggcctata gtataatata atataccctt atgttttcca agtatattgc
    12121 ctttagtatc tacaaaaaaa ccttcttcaa tacgcttttt agaatgtttt tttacataat
    12181 ttgcatagtc attatcttta acaaaacaaa tttcttgact atcaggctta ttgtgtactg
    12241 ccatacccat ttccttagca atttctctta ctctatcttt ttcatagtca cctattggta
    12301 gtaaagtatg ctctagttgc tcttgtgtaa gattataaag tgcataagtt tggtcttttt
    12361 tatctgtaac agattttttt agcaaatatc ttcctgtact ttcatccttt tctatctttg
    12421 catagtgacc tgtagctaca taatcacaac ctatttgtct agccttctta taaaaatcat
    12481 caaattttat atgtttatta caagctatac agggattagg agtttttcct tccaaatatt
    12541 catctataaa ataatctatt actttttctt taaacacttc tctaaaattt aaaacataaa
    12601 atggtatacc tattttattt gctacccttc tagcatcttc aacagcagat aaagaacaac
    12661 aaccaccttc aatttcaaca acatcatcat cttgccacaa cttcatagta acaccaatga
    12721 catcatatcc ttgttgtttt aaaagatatg cagctacaga gctatcaact cctccactca
    12781 tacctatcat tactttttta ttcataaaca aatttcacct catcttttgc attacttatt
    12841 attataccat atagagcaag tattgtactt atttaaaata tagcttgttc tattttctaa
    12901 aaaaataatc atctttatta tttgaatctt atggattatt ttgttaacta attaacctct
    12961 aaatcaagtt aaaatactta atttaggcaa taaaaaatag ccattttaaa aacagctatt
    13021 ttttatgtcc taattatatc attagtactt ctttttaatc attgtaaact tctcataaca
    13081 caatcactta tttttattct tcttcttcaa catcatggtc atgagcatca tctataacat
    13141 atccatccaa ttctggtata tggatattgt tcttttgagc ataatctata agagctgctt
    13201 ttacagcttg ttcagctaaa actgaacaat gcatctttac tggtggtaaa ccatccaaag
    13261 cttctgcaac agctttattt gtaagttcta atgcatcttt tatagttttt ccttttatca
    13321 tttcagttgc catagaagaa cttgctatag ctgaaccaca gccaaatgtt ttaaacttaa
    13381 catcctttat tacatctcca tctatgtcta aaaatatctt cattatatct ccacatgtag
    13441 ggtttccaac ttcacctaca ccacttgcat tatcaatttc tcccatattt cttggattca
    13501 taaaatgttc cataacttta tcactatatt gcattatctt ttcctcctac tcaatttatg
    13561 tctatttttt atgctttatc tttatgtcta ttttactaaa ccttgtttct tcgcatcatc
    13621 ataaagtggt gacatgcttc taagtctttc tattactttt ggtaagttct ctagtatata
    13681 atctatatca tcatctgttg taaaatctcc aactgtaagt cttaatgaac catgtgctat
    13741 ttcatgtggt aaacctattg caagaagtac atgtgaaggg tctaatgaac cagaagtaca
    13801 tgcagaacca cttgaaccag caattcctaa catatctaat aataaaagta ttccttcacc
    13861 ttctataaat tggaatgcaa agtttgcatt tcctggcaat ctattttcta agctaccatt
    13921 tactcttgtg taaggtattc tttctaacac accatctatt aacttatttc taagacgagt
    13981 taaagtttct acatgatttt gcatattttc cttagctaat tctgctgctt ttccatatcc
    14041 aactattgca ggtatatttt ctgtaccagc tcttttgctt ttttcttgtg ctccaccatg
    14101 gacaaagtta tgaagtctta caccttttct tatgtataaa gctcctacac cttttggtcc
    14161 atatatttta tgtgaagaca tactcatcaa atctattcct aattctttaa catctactgg
    14221 aatattacct gtagcttgta ctgcatcagt gtggaataat atcttatgtt ttttagctat
    14281 ttcggatatt tctttaatag gctgtatagt tcctatttca ttatttgcaa acattatact
    14341 tataagtata gtagtatctt ttattgaatc ttctagttgc tttaagtcta cttttccttc
    14401 gctatcaaca tctaaataag ttatttcaaa tccatgatgt tttgctaagt attcacatgt
    14461 atgaagtatt gcatggtgtt ctatttttga agttattata tgattacctt tgtttttatt
    14521 agcataagct acaccctcta gtgtccagtt atcactttct gaaccacctg ctgtaaaata
    14581 tatttcacta ggttcagcat ttataagggc tgctacttgt tctcttgctt tatccaaagc
    14641 atcttttgct tctcttccga atgtgtgaaa actagatgca ttaccaaaat aatctgttag
    14701 gtatggcatc attgcatcca atacttcttt tttaataggt gttgttgcag aataatccat
    14761 atacaatctt cttttttcca ttacattcat ctccaaatct attttttaat atttgttata
    14821 tcattttcta atttattttt gttatagtca tttatcatat ctttaagagt aattgaatct
    14881 ataacatctt ctataccctt tttcatcttt tcccaaacta tttttgttac acacatgttt
    14941 gagttttcac atacatcttc atctagtaca cagtcagata atgcaactgg tccttctaat
    15001 actacaagta tatctcctac tgttatgtct tcagcattct ttgaaagcaa ataacctcct
    15061 tgagcacctc tcacactttt tactagaccc gattttttta aagatgaaaa tatttgttct
    15121 aagtactgat ctgatatttt ttgcttctta gcaataaatt ttaatgatac tggtccattg
    15181 tcttgattta atgctaattc aaacattgct tttagtccat atctaccttt agtagacaac
    15241 ttcatatatt atcacctcta tatagaaatc taattcatac tgttttagta ggaattactt
    15301 tcaagataat tttattatac cctactaatt ttgtcaagat taattttaat tttttatttt
    15361 gaataaattc cccattttcc aatgttttca aatcctagtt ttttatatat ctttccagcc
    15421 tcttcattat cataaaacaa acaaggaatt ttgttttctc tcagtaactc tgaacatagt
    15481 ttaattaggc attttgtagc aagtccttta gctctatatt taggatgtgt tcctactcca
    15541 attatcattg catgagtcct attttctgaa gtactctttg ccattgacac cacctgtttg
    15601 ttaatctcta tacaatatcc tctacctgtc cttagtccat tttttaaatt ctctaaagtt
    15661 gtactgtgaa attcatctat taagtcataa agttttactg tcttttttag atttccaagc
    15721 ctaattctct taacctttgc attacaatta ttatctatca aaaacttttt agtttgaagt
    15781 ttgcaaaaat caactacctt taaattatta agtcctaatc tctttgctaa agcatctaca
    15841 gcacatattt tcccagatat ttcttcgtaa ttagtattac gaagtagatt ataaaaacct
    15901 tcaacatcat actctccatc agaataaaac atcataaatt caaaatattt aagtaatatt
    15961 gcctttattt caccatgttc acccacatct gcccatatat ttaaaaaatt atttccatat
    16021 ccatatcttt caatatcccc tattataaac aaattgtact caggttcttt ttttaagtat
    16081 ttcataactc tactgtgata agttgagtta agttttataa tcataatatc tacccccgat
    16141 acataaagtt cagtctaaaa atcactattt agatattcta aatatttttt tatatttttc
    16201 tcagttttat tttcttgtgg tatgtaataa atactatctt ttatgctatc tggtaaatac
    16261 tgttgcttta cataatgatt tttaaaatta tgaggatatt tgtatccaca acccctacct
    16321 atttctttag aaccagaata gtgagcatcc tttatactat ttggtatatc cttaacaaac
    16381 tctttatcca gttcatccat agctttcatt attgctatac atgcagaatt agactttgga
    16441 gatgtagcca aaagaatagt agcttctgct aatggtattt tcgcttctgg aaatccaagt
    16501 tgcatagctg agtctacaca tgcctttaca attacaatag catttggata agctaaacct
    16561 atatcttcac tagctattac taaaagtctc ctacatatac ttatcaaatc cccacctttt
    16621 ataagtcttg ctagatagtg tatagatgcc tgtgggtcac ttcctcttat agatttttga
    16681 aatgcactta aaatatcata atgactatca ccatttttgt catagtttat aattttatta
    16741 aatgtagatg cccttattac atctgaatct atataagtta tattatcctt atttggttta
    16801 gttgaataga ctaccacttc taaaatattt aaggctcttc tcatatctcc atcagaaaga
    16861 attgctatat cttcaatagc attattattg cactcaatat ccatatagct atcttcattt
    16921 aatacttcaa cagctctttt cagtcctttt tctatatcaa gtttttctaa aggtttaaat
    16981 tcaaatacag tagacctact taaaagagct ttatatacat aatgatatgg attttctgta
    17041 gtgctagcaa tcaaagttat actcccattt tccataaatt ctaatattga ttgttgctgt
    17101 tttttattaa aactttgtat ttcatctata tataatagga ctccatttac attatttata
    17161 cttccaagct ctgatatcac cttttttata tcttctaaag atgaattagt agcatttatt
    17221 ttatagaagc ttttattgct tctttctgct attatttccg ctactgtagt tttaccaaca
    17281 ccaggaggac caaaaaatat catatttggt aaaaaattag attgtaatat cttagacaat
    17341 atttttccat tacctattat atgactttga cctattacat cattcattgt ttttggtctt
    17401 attttatctg ctaaaggcat tttctcactc ctttttaata tacttatcat taccttaata
    17461 attcgtctaa aaaaggtatc tatttgaaat agataccttt tctaatctaa acctataaaa
    17521 tatagattat tgattgtttt ttaatttttt taattcatct gtaagtttgt cattaagtat
    17581 tcttatatga gtacctttca tacctaatga tcttgattct atcacacctg cactctcaaa
    17641 ttttctaagt gcatttacta ttacagacct agttatacca actctatcag ctatcttact
    17701 tgctacaagt agaccttctt ttccatccaa ttcagcaaaa atatgttcaa ctgcttcaag
    17761 ctcggagtag gacagagtgc ctattgccat ttgaactaca gctttctttc tcatttcttc
    17821 ttctaattct tcacctattg ctcttaatat ttcaagacca acaacagttg cactgtattc
    17881 agctattact aaatcatcat cattgaatga atttgaatat cttgaaagta ccaatgttcc
    17941 taatctttga ccgcttccta atattggaac tactgtagta tatttttgta atcttccatg
    18001 ttcttcagga aagatttcta atatcttctc accatttaaa ttttctaatg tttcatcaat
    18061 ttttaacaca ttttgagtat attcatctga aaatttcttt tgcttagtat actcatcttc
    18121 tataactgaa ctgtcttgaa catcatttaa atgaagacct agtactttac cttttgcact
    18181 tactacataa acattagaac ttaaaacgtc gcccaatgct ccggccagta aatcaaaaga
    18241 gacactgctt ccaccacttg tttgtaatgt tttatttatt ttccttgttt tttgtaacac
    18301 ttcacttgcc atttaatcat ctcctcatga aatttaggcc ttttaataac cttttatatt
    18361 ttctaatatt attttcacat ttaatgaaaa tttatttttc tattagttgt ttacacttga
    18421 aagattatca tagattggaa cctttttcaa gagaattatg tgattttttt caattttcac
    18481 tcttttccgt cattatgctc ttttgtctct ttattacatt ctttattaga acacactgtt
    18541 tttgtttcag atttagtcac tttttcaacc atatatgaac cacacttctc acaaaactct
    18601 cctgttggct tattccaagc tacaaagtca cagtttggat aattagaaca cccataaaaa
    18661 gctttacctc ttttagattt tctaagtata atatctcctt cttcacattt tggacattta
    18721 acaccaattt tattcataat tggtctagtt gttttacaat cgggatagtt tttacaagcc
    18781 ataaactttc caaatctacc atatttgata accatattag acccacagtt ctcgcaaatc
    18841 tcatcagttt cttcatccat atttactttt tctatatttt caagagcttc ttcaatagcc
    18901 tctttaagtg gtgcatatga ttctgataca atctttttcc agtctgtatt gccttcttct
    18961 ataacatcaa gtttactttc catatcagca gtaaaatcta catctataaa tttatggaaa
    19021 ttattttcca aaatatcagt aactattatt ccaagttctg ttggacatag acttgttcct
    19081 tgcttttcaa catactctct attcagtata gtagctattg ttggagcata cgtacttggt
    19141 ctacctatac ctagttcttc tagtgttttt actaaactag cttctgtata tcttgctggt
    19201 ggttgtgtaa aatgttggtc tggcaatata ttttctatat ttaatatatc tcctttttct
    19261 atacttggta aaattttgtc ttctctttct gaaaaattat ataccttggt ataaccatca
    19321 aatttcattt ttgaaccagt tgccttaaat acaatatcgc ctattttaca ttctacattt
    19381 agtatatcaa acactgaatc ttccatttga ctagctacaa atcttctcca aattaaattg
    19441 tataacttgt actggtcttt gctcaatgag gcttttatac tctcaggagt tctatcaact
    19501 gaggttggtc ttattgcctc atgggcatct tgaacctttt tagtatcctt tttttctttt
    19561 ttatcaccag tgtttttata gtagttttct cctaaattgt ttacgatata gcccttagct
    19621 ttttcttttg cttcatcaga tattctttta gagtcagttc ttatatatga tataagacca
    19681 acagtacctt caccttctat gtcgatacct tcatataatt cttgtgctat catcattgtt
    19741 tttttagtag taaaaaataa cttatttgct ccttcttgtt gaagcatact tgttgtaaat
    19801 ggttttggag ctgatttacg tcttgattta gattctatct tctcaacctt taaatcttta
    19861 ccttccattt cttttagtat ttgatttaca gcttcttcat tttctaattc aatcttttca
    19921 tcatttttac cataaaattt caaagttata tctttgtctt ttacggtctt agcctcaaca
    19981 tctattgtcc agtattcttt tggaataaat gcctttattt ctttttctct gtcacaaatt
    20041 aactttgttg taacagattg cactctacct gcactaagac cttttctaac cttttgccat
    20101 aatataggac ttatttgata acctaaaagt ctgtctagca ctcttcttgc ctgttgtgca
    20161 tcaaccaaat ttatatcaat atttctagga tgtttaattg cttttttaat tgcatcctta
    20221 gttatctcat taaactctat tctacaatcc tcagattcat ctaaatttag tatatgagct
    20281 aaatgccatg atatagcttc accttctcta tcagggtcag ttgctagata tacttgttta
    20341 gattttttag cttctttttt taattctttt attacatcgc cttttcctct tatatttata
    20401 tattgaggtt caaaattatt ttctatatct acacctaatt tacttttagg taaatctctg
    20461 acatgaccaa ctgatgcttt aactgtgtaa tgacttttcc ctaaaaattt ttctatagtt
    20521 ttagcttttg caggcgattc tacgatgact aatgttttgg ccataaaacc atacaccccc
    20581 actacctaat cttaatttat acatttaaac tgtacgtttt attattcatt tctactacta
    20641 actcatttag tactaattca ttgattattg aatttacata ctttatttct ataccagtat
    20701 aatcacaaat actatctata tgcaagtttc cttctctttt tatagcttca attatttgct
    20761 tagacttggc atttagactt atattatcat atttttgatt tattttttta tcaataatat
    20821 taaatatgtt atattcattt aaaatatcat ctatattttc aattaatttt gcaccttctt
    20881 ttataatttt atgacaacct ctactcattt gagaatttat attaccaggt attgcaaaaa
    20941 cattttttcc atgttcaaga gcaaaatcta ctgtgattag tgcaccactt tttattgctg
    21001 cttctacaac aactactcca tcacttaaac cactgataat tctatttcta ttagaaaaat
    21061 ggtacggggc tactgtcgag ttaatattat actctgataa taataatccc ccattttcta
    21121 atattttatt tgataaatgt aaattttgtt ttggaagagg attgtctact cctgaaccaa
    21181 gtactgctat agttttagat tttccactca tacaacctat gtgcgagtat gcatctattc
    21241 ctatagctaa tccacttact atattaactc catattggga tagttgacaa cttattgttt
    21301 ttgcacaatt aattccatag gctgtaggtt ttcttgaacc tactatagct atattaaaat
    21361 tattattaac taagcctatg tcacctttgt agaataataa ctttggagcg ttgtatatat
    21421 tctttaaatt ttcaggatat tctttatcat atttacaaat atatttaatc gcttttttgt
    21481 aaagtagttc ttttatattt tctaaataag cgtgacctct atattttact atattttttc
    21541 taatattcaa gcttatattt tttaaattat atatttcttt ttcagaaaaa tcaaatatat
    21601 cttcaatatt tactatttca ttttctatta tttcaattgt ttttgttgtt attccaccaa
    21661 ttgattttaa ccataaataa gtatctcttt tttccataaa atccacctca aaaaactatt
    21721 taaaataact ataataagcc tttctaaatg aaaaagcttc tataatacac tgtgaattta
    21781 tcaagtctct ttcttctaaa tctgctattg ttcttgccat ttttaaaagt tttgtataac
    21841 ttctactgct taggttgtat ttattaaata tcatctcagc cgtttttgaa gcttcttttt
    21901 ccagtttaca atatttaaat aaattgtaag ctttaatttc actattattt tttatattat
    21961 ccttcttaaa tctatttatc tgtatttttc tagcattttc tactcttctc tttatttttt
    22021 gagagctttc ttcttgtttt aaagaattaa aatctgaata ttttatagaa ttaacttcta
    22081 caaaaatatc aaacctatct aacaatggac ctgatatttt atttatgtat ctatcaattt
    22141 cattacttct acatctacac tctgtgtctg acatataata accacatgga catggattca
    22201 tagctgcaac taacagtact ctacaaggat attttgcact gtactttact ctggatatat
    22261 ttacatatcc atcttctata ggttgtctta aagtctcaag tatttttcta tcaaattcgg
    22321 ctatctcatc taaaaataag atacctctat gtgctaaagc aatttctcca ggtcttgcat
    22381 ccattcctcc tccaattaaa gattgctttg tagttgtatg atgaggtgct ctaaaaggtc
    22441 ttttatcaat tattccttta gtttcattta tcatacccaa tatactatag accttactta
    22501 cttctatcat ttcttctaca cttatatctg gtaaaattgt ccttactctt tttgcaatca
    22561 tagtttttcc tgagcctgga ggtccaatca taaacatatt atgatttcca gcagcagcaa
    22621 tttctgcact cctctttaca aaataatttc cttttacatc tttaaagtct tcatcatatt
    22681 tactttcttc attattaatc ttagtatatt tgcaatctat ataactatta tctttttcta
    22741 attcttcgtt ctcttttctt gacttatcat ccaaaaaaga cattatcctt actttatcga
    22801 cttttatctc ttcgtttaaa aaatcgacac attcttttaa acttttaata ggaattatat
    22861 caatttcatc tacaaataga ctttctttta tgttctcaat tggtataaaa atacgtttta
    22921 tgttctgagt ttttgcaccc ataattatgg gcaatatacc tcttactttt cttattttcc
    22981 catctaatga taattctcct ataaacatac tttctcttat gtaattttca tcttttttta
    23041 ttaactctct taaaaggcct atacttatag ataaatctaa aaaagctcct tcttttttta
    23101 tatctgctgg tgataaattt acaactattc tcgtgcttgg aaatttatat ccactattaa
    23161 gaattgctga ctttactctt tctcttgctt ctttaatttc tttgcctgga agaccaacaa
    23221 tattaaaact aggtattcca ttgcttacat ctacttcaac cttaactaaa aagctgtcga
    23281 ttcctactaa gttgcttgaa ttaataatac ttaacatatt taatcctttc caaaaacatt
    23341 atctttttta aaaagcattc attatgtgat ttattctttt ttctaataaa tatacctcta
    23401 taacatcaaa tcttatggga gtgttattta agttattttt tataatataa taacttgcaa
    23461 ctccaattat cttttttctt tttttgaagc ttacagattc acaagggtag ccaaacttta
    23521 tgttagacct tgatttcacc tccacaaata taatttcatc ttccattttt gctattatat
    23581 ctatttcccc tatctttaat ctgtaatttt tttctagtat ctttgctcct tttgatagca
    23641 aataattaac tgctactttt tctccaaaat cacctttttc tttattattc attattcact
    23701 ccaatacatt aaattttcac ttttaaatta tggtcagtat acaattttac tattcaagtt
    23761 tttggattga ttttatttcc atcaatatta ataaatactt ctattgactt tattctttta
    23821 tgatattatt ctagttttca taggaaaagt ttattttttt ggaggagtgc ctttaatgaa
    23881 cagagaaaaa ttagttgctt ttatacgata tttatcttta tttatattag ttgtaagcta
    23941 tttgaaaaat gttgaaaata cattgtatcc ggtcataact atgataatat tatttctaat
    24001 tataataaat aatcaagtta gatttttttc tttatcaaat gagaatacat ttatacttat
    24061 ttcttacttt ttagaactta tattaatttt tttactttgt aattatacaa aaacttttaa
    24121 ttcgatttac tttgttcctt taatattaga tatatcattt ttaataaagg aaaaatataa
    24181 gtacatattg ttctcttttg ttataatatc ttctcttgtt attagtttag ataaaaacat
    24241 atatttggca ctggagtcaa gctctatatt gctaattata actgtacttt ctatatatat
    24301 agaaaatgag aatttatcaa aattatacag tcaaaatctt tatgataagc ttagaatttc
    24361 taaagatgaa ttaaaaaaag ttaatgctga tttggaaact tatgcaagtt ctatagaaga
    24421 attagcaatt ttaaaagaaa gaaatcgaat atcaagagaa atacatgata gtgtaggaca
    24481 ttctctttct acaacaataa tacaattaaa tgctatagaa aaactcttaa aagacaaacc
    24541 tctaatttat gacttagtac atgaacttag agaatttgta actgaaagtt ttcaagatgt
    24601 aagaagggct atatctgagt taaaacctgt tgaatatgaa aattaccaaa gtttatttaa
    24661 aatcaaagaa ttagttaaaa gctttattaa gcttacaaat attaatgtta aattaactat
    24721 ttcaaaaaat acttggaact tatccagaaa tcaatctata actttatata gacttataca
    24781 ggaatccctt tccaactcat ctaggcatgg aaaggcaact gaaatcagaa tttttattac
    24841 attcaatact tcaaatctta taattactat tagtgataat ggtataggat gtggcaatat
    24901 aaaaaaagga aatggactta atagtatcaa tgagagaata tatgaattaa atggtaaagt
    24961 tgaatttgat aattctaata atggttttac aattagagct tcttttccta aattttcagg
    25021 gggtgatttt tttgaataaa attaaagttc ttatagtaga tgacgaaaaa ttaattagaa
    25081 aaggtcttaa aattatatta tcatcttata atgatttaga aattgtagga gatgcttcta
    25141 atggatatga agctttagaa ttttgtaaaa tgaatgatgt agacatagtt cttatggata
    25201 tacgaatgaa agtatgtgat ggggttctag gtacaaggct tataaaggaa tataataatt
    25261 ctataacatt attaatttta acaactttta atgatgatga atatataaaa gatgctatga
    25321 agtttggtgc ttcaggatac cttttaaaag atagctctga tgaagtattg catgaaggga
    25381 ttcgttcttc attttttggc aatattgtgc ttgataaaag tgtagctgaa aaaatcatga
    25441 cctctgaaaa aacggttaaa caagagcatc tttatgacat gtacaattta acagaaaaag
    25501 aaatatctat aattagacta attgcaaatg gacttaataa taaagaaata agtcaagaac
    25561 tattcttatc tgaaggtaca ataaagaata acataactaa tattttagct aaactagaac
    25621 ttcgtgacag aactcaacta gcaatctttg ctttcaaaaa taaaattgta atagaataaa
    25681 tctaaaaata taaagtgact taagtcactt tttttagtga cttatagtaa tagaattata
    25741 attcacaatt ctttattata atattatagc tttttaactt atacaaaatt attttggagg
    25801 gattactatg gatatagtca gagtaaataa tataacaaaa agatttaatg ataaattagt
    25861 acttgataac atcagctttt ctgttaaaaa aggtgagata tttggactta ttggcccaaa
    25921 tggtgcaggt aaatctacac ttataaatat aattacaaat cttatgttac caaatagtgg
    25981 tagtattcaa atcaatggtt tagatttatc aaaagattat ataaaagcaa agtctataat
    26041 agggttagtt ccacaagaat tagcaattat agaaactctt acttcttttg ataacttaga
    26101 gtactttgga gctttctacg gtttaaaggg aaaactactt aaagaaagaa tcattgaagc
    26161 attagaagtt acaggtctta ctgaagtcaa aaagaaaaag gtcaaaaaat tatctggtgg
    26221 tatgcaaagg agattaaata ttggaatagc actcttaaat catcctaaaa tattaatatt
    26281 ggatgaacca actgtaggtg tagaccccca atcaagaaat catattttca attttataaa
    26341 agatatatct aaaaaacatg aaactactgt aatatatact tcccattata tggaagaagt
    26401 tgagcacctt tgttctaaaa tttttattat ggatgaaggt aaagaaattg cttttggaaa
    26461 taatgattat ttaaaatcac tcgtatctac aaatacaaaa ttaattatgg aaataaaaaa
    26521 tataaatgcc caacttatct ttgatttaaa aaatactaaa ggtgttatat ctgttttaga
    26581 aaataattca ttattagaat tggatataga taaaaaatta caacttacag atatactctc
    26641 tgtcattgac aaaaacaatt caaaaataat gaaaatttct tatgaagaac ctagcctaga
    26701 agacgtattt ttaaatctaa caggaaaaaa tttaagggac taatggaggt gattttatga
    26761 aattatacat gagtgtaaaa actatgctaa agagtttaaa aagctccttt atacttaatt
    26821 taatatattt tttagctttg cctctgattt tatcttggtt tcttgggatg gttacagaaa
    26881 gcatgtttca aaaccctata aaaacagaat ctacccccat tgttatatat gacaaagata
    26941 acacaagatt atcaaatgat ttgacaaagt atttgaaaaa tgatttatct tatatattaa
    27001 cagttaaaaa agatgattct aaggcagaat taaagcttac tatacctaaa ggctatgaga
    27061 gtagtttatt aaatgaaaaa tcaaatactc taaacataga aaaacttgga actcgtgatg
    27121 atatcgcaat attgcttcaa gatatattag atacttacca tgaaaaattt tatttaaata
    27181 attctcagaa aataagttca gaagattttt ctaaattatt taataaaaat tctattgata
    27241 cttctataat aaataacaat gttaaacaga gctcttatga atactttgct ttagtttcac
    27301 taggatttct tgtaataatt tttataatga acaatatttt gtcaaattat ataagtgaat
    27361 caaagggact ttcaaaaaga ctttattcta tgcctataac tagagtacaa ttcttaattt
    27421 atgactttgt aggtttatgg atttattcat ttatattctt attattatat gttttattct
    27481 ttagaatact tggaataaca tttaaaggta attttgccat attgctccta ctttgtgctt
    27541 tatcatctta ttttatgact tcaatatcta cctttgtaac cagctttttt agcaaaaaat
    27601 acggtacagc aatagtttat gcattactct ttttacagac aatttttgga ggtatcttca
    27661 gtatgataag tgatacattt actaagctta caagtttatc tcctacctat ttaataggag
    27721 aattgtttag taattatgaa acatttaaaa ctatagattc aataggagat ttaattttca
    27781 catgtttaat tatctctaca atattaattg cacttgctat tgtaaaggaa aattataaat
    27841 ggagggaggt ttaataatga ttagattaac tattgcaaat ataaaaagat atctaaaaaa
    27901 tcatactctt ttaattaata tggttatgct tcctattata ctaatttttt cacttaattt
    27961 ttttatcaac aactcgggaa atcaatcaat gtatttttct ccagtagcaa ttgtatctga
    28021 ctcttcagga aagtatgaac ataagttaat aaatagttct aaattaaaag aaaattcatt
    28081 tagattaaat gaacaagata aggctatgaa tctattaaaa aataataagg tttctgctgt
    28141 attcgtttta gataaaaatt tttcaaatag tatagataaa ttacaacgac ctgtagttaa
    28201 atgctttaaa attgaaaatg gtggtggtag tctttgggca gaatcacaaa tagaatcttt
    28261 tattactaaa tcgttaaaat tgaaagtgga taaaaatata gatgataaat tgacaaagac
    28321 aaatataatt gataacaaac cagataagaa taaaggttca tttttagtag tcttcttgat
    28381 atgctatttt atgtatatta atgcggcaca tcttgcttct gatttattta cattaaaaaa
    28441 atcaaatgtg ctaaaaagac ttatctctac agataataaa gatataaaaa taattttcag
    28501 tatattttta ggactatttt ttattcaatc aattgtatac acttttgctc tattatgttt
    28561 ttcaattata gaagacttta atttgagtct gaatgtattg atgattgtac tagcaaatag
    28621 ttttgtttcc actggatttg tattttgggt agctagagta tttaaaaatg aaagttctat
    28681 aagtcttata tctacatttt attctttaat aggtctagct atatctatct ctagtctaat
    28741 accatcaatg gataaattat cctttatgac taatctttca aagttcacac cattttattg
    28801 gactatagat gctattaaaa acaatggtag tattttaata aatataatag ctctaatatt
    28861 aattggaatt atatttgtaa ctgcaggaag tcttaaacta agagattttg ctaaaaacta
    28921 ataattatta aatataatta gtatctattt ttatataatt gtaaaagaat atttcaacat
    28981 attaaattat tttatcttat atttttctct ttagtttcac acatcaccaa gtctaaactt
    29041 tatattatat attaatattt ttgacttgtc aagttattta tgttataata ttaatcatta
    29101 gataattaaa gcattgtacc taagttgcaa tatctaaaat tcaatttgga ggtataacta
    29161 gcatatgtat aaaattggtg aaagtgtaat gtatccaaaa gagggagcct gctctgtaaa
    29221 tgatattgta acaaagaaaa taaatcatga gatgcaaaaa tactatgaat taagtgtcat
    29281 ttttaatagt aaccttaaaa tttcgattcc agttctaaat gcagatagaa ttggcatacg
    29341 tccagttatg gatggaaatg atgttgataa ttttatccaa tctattaata aaacagatgg
    29401 tgtatggatt tttgatagaa aagaaagact taaactgtac caagataaat ttcattcagg
    29461 agatgttttt gaaattgtaa aattaatcaa aatgcttatg atacaagaca gttcaaaaca
    29521 attgtgtagc acagataagg agtttttaaa taaagctcaa aaatttgctt tatcagaatt
    29581 agctgctgct caatgtaaat cctatacaat ggtattagag gaaatgaaaa aacatatatt
    29641 aaattcaaag aatataaatt aaaccagttt tttaaactgg ttttattttt tatactaatc
    29701 atccatgcta ctttagctta ttttaaactg cctaatctaa tattaataaa gttatctcaa
    29761 ttagatagat gtattttatt caaataaaaa aaaactaaga gtaaatcata ttactgattt
    29821 actcttagct aataaaattt aatcttcttc aagttctttt ttctcttgta ataatacact
    29881 taattcattt gcttctaaaa cttgtgcctc tgcaatatca agtaactctt gttgccattc
    29941 cacacattta tctttgtaat caaaaggtag cgttctgtca actatgtact tatcaataca
    30001 gtcctccgca cttttaatta tttcttttct ttcatcaata aatttttcta attctactaa
    30061 ggcttcttca taagcttttc tactcatata aattccccct ttgtacaatt attctacatt
    30121 aaaatgattt ttcctctttt ttgtataaaa ttttatttgt atataacttt tccatcaata
    30181 tcttttatag tttgacatcc agttaatatc atagaactac ttagctctga ttgaagttta
    30241 tttacataag tttctacacc atcagttgca ccaccaaaag aagcagtcac aaaaggtctt
    30301 cctattaata ctgcatctgc acctaaacct atcattttta gtacgtctaa accagttctc
    30361 actccaccat ctgcaagtat tgtaacttta cctttaacag catcagcaat ttttggtaaa
    30421 acttcacatg ctcctggtgt acagtcaagc actcttcctc catgatttga tacgactatt
    30481 gcatccacac ctgcttctac tgccattaaa gcatcttcaa cagtcattat acctttcaat
    30541 ataaatggta gtttagtaga ctttactaac tcctttattt gtttaacatt tttaggaagt
    30601 actggttttc catgtaatga taaagttact agaccacagg catctatatc aactccaact
    30661 gcaaatgcac cagcttcttc agctaatctt atctttttta taatattttc attatcccaa
    30721 ggtttaataa atacaattcc agctccatta tactttttga ctacatctaa attttccatt
    30781 aaaaaagcat ctactgccgt atcaccaacc attgcataaa taccactatt agcacatcct
    30841 gcaactactg gttctatgta atctctttca ttaatttttc cacccatatt taaagtagtt
    30901 cctgttactg gagctgcaaa tataggtgca ctcattttct ttccaaacat ctctatagat
    30961 gtatcaggat ttgatacatc atgtattact ctcatattta cttttacttt ttctaaactc
    31021 ttgctatttt ctataaaaga agaaccactt cctttacctc ccatgccagg cacttcacct
    31081 gcacaagcaa ccccattaca aaccttacaa actttacaac ttccatttaa attttctcta
    31141 gcacttttta ataattcatt atattccatc tttaatcccc cattaactat agtaatttat
    31201 attatataca ttatatcata ttatatttac agtatatttt tcaaaaaact ttttctgtgg
    31261 ataggagtta tgccatattt ttcaatagct tcatagtgtt ctttagttcc atatcctttg
    31321 tgagatttaa acccatattc tgggtatact ctatcatatt ggtacattat actatcccta
    31381 gtaactttag caagtatact tgctgctgct attgatatag attttgaatc tcctttcaca
    31441 attggatttt gagatatgtc aatacctggt attgttgctg catcaactaa taaataatcc
    31501 ggtgcttttt ttaaacaatt tatagccttt ttcattgcca tatatgtagc atttaatata
    31561 ttaaattcat ctatttcttc attgtttact atacctattc cataatctag tgcttcttct
    31621 tttattactt caaaaagttc atctcttttt gcctcactca gtttcttaga gtcatttaca
    31681 ccttctattt tggtatcttt tttaaatact acaacagaag ctacaacagg tccagcaagt
    31741 ggtcctcttc ccgcctcatc aattcctcca atatataaat aacctttatc atagccttcg
    31801 ttctcaaata tatttatagt ttccaatctt tcttcttctt ttctaatatt atccagcttt
    31861 tttgctagtt ttatagctag accttgtaca gattttcttt catcaactct taacagctct
    31921 atatattcca tatatttttc tacttctaaa gtctctatta tttctttaat ctccctcaca
    31981 cttttatcct gcataccatt ccctcatctt tcgtttttaa aaatttaatt tttaaatata
    32041 ttaaaatttt ttaattagag tttaattgca tccactatag ccacatttta aacatatact
    32101 acatccacct gtatgtgcaa ttccttcact tccacactca gggcacacaa atttttcatc
    32161 ttcttcaaaa tttttattta ttatttcttt ggctttttca tctttattat ctttttcttt
    32221 tttattattt ttataatatt cttttttact attttctttt ttatcttctt tattattttc
    32281 ttcctttttt tcatttatta aaattccact ccttttacat ccatctctaa atattgttac
    32341 tcctttaaga ccagcctccc atgccttaat atatatattg aaaacatctt caactgtagt
    32401 ttcaaaaggt atatttattg tagatgatat agaagcatca atatactttt gccatacttt
    32461 ttgcatgttt attctttctt catatcctat attcatagca gtaacaaaaa aatctggtaa
    32521 acattcttca tctgtaatac ctttcttgtc catatactct ttaacaattg gagtatatac
    32581 tttataatac acatcctcat catgcaagct ttcagttttt cttatatatg aaagattaaa
    32641 tataggttct attccaccac tgatacctat cattgtagat atggaacctg taggaggtat
    32701 ggttaaaagt tgtgaatttc taagcccata ctttttaacc atttccttag tgtcttcatt
    32761 tagattttct ataaaaaact tagatttaga aatattttca tatttatact cactaaatgt
    32821 tccatattct ttagcaatta atgctgattg cttcacagct tcatttaaca tagtcttacc
    32881 aattaaatca catagttcta aagattcatc agaaccatat cttattccca tttttataag
    32941 catatcagct atacccatta cacctaaacc tatctgtcta tacttatgta cactatctct
    33001 ttgttcttgt aatggatgca agttcaatcc ctcatctaag acatcattta aagctactac
    33061 agcttctcta acgcaagatt ttaatttatt aatatcaaaa gtagcatgtt caccaaatgg
    33121 ttgtattata aactctgaca agtttattga gccaagtaga caacttcctc cacttggaag
    33181 tggttcttct gcacaaggat ttactcctga aaagcaaaag ttcttatctt cactcataaa
    33241 attatatgtt tttattttat cccaaaacaa tatacctggt tctgcataat cccagttatt
    33301 tttcacaaat ttattaaata aatcataagc atttactatc ttaactattt tttctccagt
    33361 agtttcaact tcaaaagtac aattatataa ctttttttct cttacagctt gcataaattc
    33421 atctgttact cttacagaaa tatttgcttt agtaaccttc tccaaatctg tttttatatc
    33481 tataaagtcc tctatctctg gatgatttac atccatagaa atcataagag ctcctcttct
    33541 ccctctttga ccaattaagc cagttgtcat actgtaaagt tccataaaag agactgctcc
    33601 agtcgtatgt tttgcagcat tgttaacttt cgctcctttt ggtcttagct ttgaaatatc
    33661 aattccaact cctccaccat aactaaaagt tctagcaagt ttttttgcag tatcaaatat
    33721 atcttctatg ctatcttttg gtggttctat tacataacaa ttagaatatg ttactttttt
    33781 tccttctttc tgtaatcctc tatttgctaa aattcttcca gcaaatagaa atcttttttg
    33841 tcttattagt tttgatattc ttgaactatt atttgaaatt ctctttatcc attcatcaaa
    33901 actctcacca ttatgtctat acttgttctt ccatattgat ttttgtaatt catcaaactc
    33961 ccaagcttta gtccttattt cagagcgtct ttctctatat aatatgtagt gttttgctat
    34021 atcttttcta tttgattcca taagtagtac ttcaactata tcttgtattt cttctacgga
    34081 taagcatttt tccatagaat tatagtcatt agctattttt cttgatatat catatgccaa
    34141 ctctctatct actccacctt cactatttat actagcttta tagacagcat cttcaatctt
    34201 acttttacta aaaagttctt ttgtaccatc tctttttatt atgtacttca tttttaaacc
    34261 tccaataaat tagtattaaa ttaatacaca cattaactat aactctaaac aaacttttta
    34321 tatatttgta tattttcata ttaatt
//
LOCUS       DAELLV010000015        32739 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.015, whole genome shotgun sequence.
ACCESSION   DAELLV010000015 DAELLV010000000
VERSION     DAELLV010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32739
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.015"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1485
                     /locus_tag="KPD58_000649"
     CDS             <1..1485
                     /locus_tag="KPD58_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HBG0264436.1"
                     /translation="KEEKNYWDEYMPLYEELNSYFYNAIVNSKFKYDLIKEFGEQFFT
                     IVEYSLKSFSKEIISELQEENKLCSEYTRLLASAEIMFDGKIRNLSGMGKFMYSKNRK
                     TRELANKAYYNFFEENETKFDDIFDKLVKLRDKMSKKLGFEDFVELGYVRMMRSDYRE
                     YMIKNVRKQVLKYVVPMANELYEKQAKRIGLEYLSYIDEGVEFLTGNASLKGDSRYII
                     KNGKRMYSELSKETNEFFDFMLENELMDLETKKGKGAGGYCTYIPDYKSPFIFSNFNQ
                     TADDIDVLTHEAGHAFQLYMSRWIDMPEINFPTLDSCEIHSMSMEFITWPWMDLFFKE
                     DTDKYKFTHLSSSIKFIPYGVIVDEFQHYIYKNPNVDKSKRKEIWRFLEKKYLPHRKY
                     GDNSFLERGCWWFKQGHIFKNPFYYIDYVLAQICALQFWKKMIQDRDAGWKDYINICK
                     VGGTKSFLDIVSMGNLYSPFDDGCIESIIGDVKSWFDEINDSKL"
     regulatory      1817..1927
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            2031..2402
                     /locus_tag="KPD58_000650"
     CDS             2031..2402
                     /locus_tag="KPD58_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HBG0264437.1"
                     /translation="MIIITNNPKVKEEVQGREVLFKDTTYIGILEASRDLIHEGYELL
                     SHPLYGSVKPNETPYRTVILKKGNRLDINSLTLIEEAIITASKFQNNKKTPKWTESVQ
                     DDFRVIDYDIFYNTIQRMQYE"
     gene            2499..3446
                     /gene="trxB"
                     /locus_tag="KPD58_000651"
     CDS             2499..3446
                     /gene="trxB"
                     /locus_tag="KPD58_000651"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBG0264438.1"
                     /translation="MENVYDLVIIGSGPAGLAAGLYGARAKLKTLILEKDKTGGQIVI
                     THEIANYPGSVPNATGPSLIARMVEQCKEFGAEMLRDNIVDTELDGDIKVLKGEKAEY
                     RAKAVIIGTGATPRKIGCPGEKELTGKGVSYCATCDADFFEDFEVFVVGGGDSALEEA
                     MYLTKFARKVTIVHRRQGFRCAKSVEEKAKANPKIEFLLDTVIEEIKGDGILESVVFK
                     NKVTGETHEYFADEEDGTMGVFVFVGLDAQTDLFKGKVDMDEKGYIITDEDMRTNIPG
                     VFAAGDCRSKTLRQVVTATNDGAIASIVAEKYIDEKFGN"
     gene            3550..3867
                     /locus_tag="KPD58_000652"
     CDS             3550..3867
                     /locus_tag="KPD58_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HBG0264439.1"
                     /translation="MLDLDKATFEEEVLNAEGFVFVDFWSEGCEPCKALMPDVHKLAE
                     TYGDKIKFCKMDTTKARRLAIKQKVLGLPTMAIYKDGEKVDEVTKDDATVPNIENMIK
                     KYL"
     gene            4136..5422
                     /locus_tag="KPD58_000653"
     CDS             4136..5422
                     /locus_tag="KPD58_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HBG0264440.1"
                     /translation="MRLELGKIFIKDIQFGDVTEVKDGVLFINKQEMLQEIGGDEHIK
                     SIDIELARPGESVRITPVKDVIEPRVKVEGNGGIFPGIMSKVDTVGEGKTHALKGVAV
                     VTTGKIVGFQEGIIDMTGEGAKYTPFSKLNNVVVIAEPIDGLKQYAHEKAVRMIGFKA
                     AMYLGEVARNLTPDEVSVYETKPLLESIKEYPELPKVGYVYMLQTQGLLHDTYVYGVD
                     AKQIVPTLLYPTELMDGAIVSGNCVSACDKNPSYVHINNGVVEDLYARHGKDINFVGV
                     IITNENVYLADKERSSNWTSKLCKYLGLDAVIVSQEGFGNPDTDLIMNCKKIEMQGVK
                     TVIVTDEYAGRDGGSQSLADADVRADAVVTGGNANQVVVLPKLDKVIGHLEVVDVIAG
                     GSDGSLRADGTIEVEIQAITGATNETGFGHLTAKGY"
     gene            5534..6007
                     /locus_tag="KPD58_000654"
     CDS             5534..6007
                     /locus_tag="KPD58_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:5666..5668,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HBG0264441.1"
                     /translation="MSLLSNKKVLIIGDRDGIPGPAIEECVKTVEGAEVVFSSTECFV
                     UTAAGAMDLENQNRVKDAADKFGAENVVILLGAAEAEAAGLAAETVTAGDPTFAGPLA
                     GVALGLSVYHVVEEPIKSLFDESVYEDQISMMEMVLEVEEIEEEMSGIREEFCKF"
     gene            6039..7349
                     /gene="grdB"
                     /locus_tag="KPD58_000655"
     CDS             6039..7349
                     /gene="grdB"
                     /locus_tag="KPD58_000655"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:7086..7088,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HBG0264442.1"
                     /translation="MGKLKAVHYINQFFAGIGGEEKADTKPHVAETLPPISLQLDKLL
                     GEDIEIVGTVVCGDSYFNENIDSASEEVLSMVKGFEPQLFIAGPAFNAGRYGVAAGTI
                     TKVVKDALNIPALTGMYVENPGADMFKKDVYVVETSDSAAGMRKALPKIAKLAVKLAN
                     GEEIGTPKDEGYIARGIRVNYFHEDRGSKRAVDMLVKKIKGEPFETEYPMPNFDRVDP
                     SKAVKDLSKCKIALVTSGGIVPKGNPDRIESSSASKYGTYSIAGVMDLTEETYETAHG
                     GYDPVYANLDADRVLPVDVLRDLEKEGVIGKLHETFYTTVGNGTSVANSKKYASEIGA
                     ALVADGVDAVILTSTUGTCTRCGATMVKEIEKTGLPVVHMCTVVPISLTVGANRIVPT
                     IAIPHPLGNPALDPTEEKALRRGLVEKALNALTTEVDGQTVFEK"
     gene            7605..9137
                     /locus_tag="KPD58_000656"
     CDS             7605..9137
                     /locus_tag="KPD58_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III family protein"
                     /protein_id="HBG0264443.1"
                     /translation="MTYPVLKGAGYVLIHTPDMIVQNGSTCTVERATNPDSEFLKEVS
                     NHIRSYEDVVNYMPNQVYIGNRRPEELRDLPMPWCEQKIEGTRNGKFGEIMPQDEFIA
                     LMQISDAFDLVKLSQEFIDEVKPKIENNYPEIAPFVGKLKGDDIEEGKELVATHIAEG
                     LYHDGKFVGYVKRAHDVDVNLNAHTMFENLVVKASGVLSAIQMLRHSKIDPAEIDYVI
                     ECSEEACGDINQRGGGNFAKSIAEIAGLQNATGSDTRGFCAAPTHALIQAAALVKAGI
                     HKNVMVVAGGASAKLGMNAKDHVKKGLPVLEDVVGGFAVLVSENDGVNPVIRTDLTGK
                     HTVGTGSSPQAVMTALITSGLDRANLKITDVDVYSVEMQNPDITKPAGAGDVPEANYK
                     MIGALAVKRGDLEKKELKDFVSNKGLPGWAPTQGHIPSGAPYIGFLIDDLTTGNRNRA
                     MIVGKGSLFLGRMTNLFDGVSFIAERNTGVTEETSGISKDEIKKIIAESMKKLALDML
                     EE"
     gene            9148..10293
                     /locus_tag="KPD58_000657"
     CDS             9148..10293
                     /locus_tag="KPD58_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HBG0264444.1"
                     /translation="MSKKVIADVFLEVANAIESGEFGKKVKIGVTTLGSEHGVENMVN
                     GAQLAKSNLFDIVLIGPKVETDLEVVEVNDEKEMHAKMEELLDSGYIDACVTMHYNFP
                     IGVSTVGRVITPAKGKEMILATTTGTSATNRIEAMVRNAIYGIATAKSMGNKCPKVGI
                     LNVDGARQVEKCLKELKDNGYDMEFADSIRADGGCVMRGNDLLVGAPDVMVTDTLSGN
                     IFMKVFSSYTTGGDYEAQGFGYGPGVGEDYDRKVLIVSRASGSPVVANALKYAYDVVK
                     GDISNVARNEFAKVKKAKFDDIISSLTKKEVKAEKVEVKMPDKEIVTRQIAGVDIMDL
                     EDAVSELWKNGIYAESGMGCTGPIVLVNEAKGDLAVETLVKAGYTAK"
     gene            10603..11682
                     /locus_tag="KPD58_000658"
     CDS             10603..11682
                     /locus_tag="KPD58_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG0264445.1"
                     /translation="MSRVKNVVELLETKGVDALYLTKKTNVNYISGFPDEEAYAVICK
                     DGNFLVTDSRYMELAEKVCKDFEIINWHNFDRSVAKAVKSVCDKVGIKKLGFERTNIV
                     FDKYEELKNLIEKDNGELIPTENIVETLRYVKDKDEIKNTRKACEIADKALEELIPHI
                     KAGVSEIELATKLEYFMKMNGAQNIGFETILISGAKTSLLHGKPSDKIIEKGDFVLID
                     YGAMYNGYISDTTRTFIVGGASEKQLEIYNLVKEAQNVGVENMKAGVHATIPDAEIRK
                     VVKKYEDYYYQGIGHGVGRDVHEEPFIGNYGDKIIEEGCIITMEPGIYFPGWGGVRIE
                     DTVLITKNGPERLTKFPKDLMILDK"
     gene            11795..12394
                     /locus_tag="KPD58_000659"
     CDS             11795..12394
                     /locus_tag="KPD58_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264446.1"
                     /translation="MYKKHKFIKNIKKAWYKIKLIDRCLIIVMAILMFQSIYNLFVNE
                     VNSQDTTTIDVVVRTTSAAIFGYFLSANFIKRPLRKYTNTDVSSNLFIDNNLSPKGQE
                     SNQNNIMNVKNTIGFTSEADNYEKKIPINNNEGFEEGETSELQIIIATVICIVALTVL
                     FVVRNFTTTTSASLGTISQMRDFVSGCVGFLLGCPSKPD"
     gene            complement(12580..13452)
                     /locus_tag="KPD58_000660"
     CDS             complement(12580..13452)
                     /locus_tag="KPD58_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG0264447.1"
                     /translation="MNINYLYYFQTVCKYKNMTKAAESIHISQPSITLAIKELEKELG
                     FELFYRIGNKIELTPEGKIFLDKSKHFIKQFEDFQCDALDLGKKRKASLKIGIPTVLG
                     TFLLSKILPRFNVIYPDIELKIFEVPTFVGAKMIEESTLDFCIGIIDSDIYDDIDSKT
                     IYKTELYLVTNPKNELAKHPIISNYMLKNVPFVILSEGSYHYKIITKRLEKAKPNIIL
                     HSNQLSTIRYLLENDLASTILYKEIFQNTENLCSIPLERAITANIGVLWRRNQYISHS
                     MKLFIEYMASIHIN"
     gene            13869..15272
                     /locus_tag="KPD58_000661"
     CDS             13869..15272
                     /locus_tag="KPD58_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264448.1"
                     /translation="MKGSDLVKFELMDFLMNPFVLMFAAVITGILFGKIKFGKFNFGV
                     SGALFTGLFIGWLAYSLGNLIIAKGETAAGYKAATVMMGNGIISSDFFDFFLIIFVAA
                     VGLLAAKDMKAVLKKYGARFVILGVLITFIGGSMTYAMTLLSSDKGSSAYEVSGVYTG
                     ALTSSPGLAAALETAGKHAEDVSKEFEKASIKDKKEILKVVDPEGKLDVNTTTSLTQE
                     QIDKYIAYAEAGVGIGHAVAYPFGVLIVILGVNFLPKLFRMDLKEERRKYEKEMKEAR
                     DSVSGKTIPEVPFNIITFFLTCLAGYLVGGIHVFMGPLGYFTLGATGGSLIVSLVLGY
                     IGKIGVVNFRMEEKVLNILKQIGLVFFLAIVGLRYGGKVVDSIMTSGMHLALVAIAVG
                     VTAMMIGFLVGKYVFKLNWILLSGAVCGGMTSTPGLGAAVDALDSDDPAAGYGATYPF
                     ALLTKVILVIVLHKLPM"
     gene            15299..16843
                     /locus_tag="KPD58_000662"
     CDS             15299..16843
                     /locus_tag="KPD58_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HBG0264449.1"
                     /translation="MNNEGAFEELSGRLRNKELCDLIMNADEAAKIIEDGMVIGVSGF
                     TPSGYPKAVPLAVSERAKSGEKIKLTVYSGASLGPEVDGAWSEAGIIERRLPYQTNSI
                     LRNNINKGVVDYIDMHLSHSTQFLNYGTIPKVDVAIVEALAITEEGNIIPTSGIGNSP
                     SFIKSADKVIVEINLAKPMEMEGMADIYITENPPNRKPIEINHPKDRIGTTYIPCGLD
                     KIAGVVITNMQDKTRPLGVVDEASKKISNNIIAFLREEVKAGRLSKNLLPLQSGVGSV
                     ANAVLYGLCESEFENLTCYTEVVQDSMLDLIRMGKVTMASTTSVSPSPEGLIKFEKDI
                     DFFKDKIILRPQEISNNPEIARRIGVIAMNTAIEVDIYGNVNSTHIMGSKMMNGIGGS
                     GDFARNGAITIFSTESIAKNGDISSIVPMVSHVDHTEHDVMVIVTEQGYADLRGLAPR
                     ERAIKIIENCAHPDYKEQLRDYLNRACKSGAKQTPHILDEALSWHSKFMSTGTMKKAE
                     TFKSAL"
     gene            16874..18265
                     /locus_tag="KPD58_000663"
     CDS             16874..18265
                     /locus_tag="KPD58_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HBG0264450.1"
                     /translation="MEKAVENFEDLSKEYINGYIERARKAQREFECYTQEQVDKIVKI
                     VGKVVYYNAEYLAKLAVEETGMGVYEDKVAKNKSKAKVIYNNLKDKKSVGIIDIDRET
                     GITKVAKPVGVVAAITPCTNPIVTPMSNAMFALKGRNAIIITPHHKAIGCSTKTVEMI
                     NEELEKIGAPENLIQILDQQSRENTRNLISSADVVIATGGMGMVKAAYSSGKPALGVG
                     AGNVQCIIDRDVDIKEAVPKIIAGRIFDNGIICSGEQSVIVAEEMFDKIMDEFKNNKG
                     FIVRDKVQKEAFRNAMFVNKSMNKDAVGQSVHTIAKIAGVEIPEDTKIIVIEADGPGE
                     EDIIAKEKMCPVISAYKYKSFEEGVAIAKANLNVEGKGHSVSIHSNTVKNIEYAGENI
                     EVSRFVINQCCATSAGGSFFNGLAPTNTLGCGSWGNNSISENLDYKHLINISRIAYYM
                     PENEVPTDEELWG"
     gene            18458..19954
                     /gene="abfD"
                     /locus_tag="KPD58_000664"
     CDS             18458..19954
                     /gene="abfD"
                     /locus_tag="KPD58_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyryl-CoA
                     dehydratase/vinylacetyl-CoA-Delta-isomerase"
                     /protein_id="HBG0264451.1"
                     /translation="MALMTGAQYIESLRKLNTKVYMFGEEVKNWVDHPMIRPSINCVA
                     ATYDLAHDPEYADLMTVTSNITGEKINRFGHLHQSVDDLIKKVKMQRLCGQKTASCFQ
                     RCVGMDAFNAVYSTTFECDKAHGTNYHDNFVKYLTYIQENDLVVDGAMTDPKGDRSLS
                     PSAQPDPDMFLHIVERREDGIIVRGAKAHQTGSINSHEHLIMPTISMTEADKDYAVSF
                     AVPSDAEGVFMIYGRQSCDTRKLEEGADVDLGNKEFGGQEALVVFDNVFVPNDRIFLC
                     GEWDFSGMLVERFAGYHRQSYGGCKVGVGDVIIGAAALAADYNGANKASHIKDKLIEM
                     THLNESLYCCGIACSSEGHKTEAGNYQIDLLLANVCKQNVTRFPYEIVRLAEDIAGGL
                     MVTMPSEKDFKSDLKVGTSGMTIGEVCNKYFKASSVASTEERMRILRFLENICLGSSA
                     VGYRTESMHGAGSPQAQRIMISRQGNINQKKELAKKIAGIKKEEALNL"
     gene            20089..20364
                     /locus_tag="KPD58_000665"
     CDS             20089..20364
                     /locus_tag="KPD58_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264452.1"
                     /translation="MTCGVRFCGGCNPRYERGKALTIIKEHFNGRVDFSIAQENVEYD
                     LLLIIGGCATLCASYQQYKVKNECVKMWDFSHIERVINVIESAICET"
     gene            20388..21695
                     /locus_tag="KPD58_000666"
     CDS             20388..21695
                     /locus_tag="KPD58_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HBG0264453.1"
                     /translation="MSWQELYQSKLCSATEAVKQIKNGDTVVFAHCVGEPPALVEAMI
                     ENAEQYKDVEIKHMVSLGSGGYTAKGMEAHFRVNPMFVSGNVRKAIENGDGDFTPAFF
                     HEVPKLLREKRLKCDVVLAQVTPPDEHGYCSLGTSVDYTYEAIKTARTVIVQVNDQFP
                     RTYGEVVHVSEFDYIVEKSQPLFELQPAKIGEVEEAIGKNCASLIEDGSTLQLGIGGI
                     PDAVMLFLTDKKDLGIHSEMISDGTLALYEKGVINGKYKNFDKEKMTVTFLMGTKKLY
                     DFANNNPAVEVKPVDYVNHPAIIMKQHKMVSINSAIQVDLMGQVVAEAMGLRQFSGVG
                     GQVDFIRGVSMGEDGKAIIAMPSITTKKDGTVISKIVSIVDEGAPITTSRNDVDYIVT
                     EYGIAELKGKSLRERARNLINIAHPSVRESLAVEFEKRFKEKY"
     gene            21715..22833
                     /locus_tag="KPD58_000667"
     CDS             21715..22833
                     /locus_tag="KPD58_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate dehydrogenase"
                     /protein_id="HBG0264454.1"
                     /translation="MEALRVVPKIFYFDTFKEFNEEFKIGKNDLVITNEFIYEPYMKP
                     LGIDTNLIFQEKFGTGEPSDEMIDSMTKEMKKYNFDRIIAFGGGTIVDICKILALDVP
                     EKSIDLFEGEVSPKKVKELVVVPTTCGTGSEVTNVAIAELKSKHTKKGLAVEETYADY
                     AVLIPETVKGLPYKFFVTSSVDALIHAIESYLSPKASPFTEMYSLQAIKMIMDGYKKI
                     VDKGEEERFNHLRDFVLASNYAGIAFGNAGCAAVHALSYSIGGAFHVAHGEANYQFFT
                     EVFKMYSRKKPNGKIIKCTKILADALECDPNCDVYGELEKFLNKLIAKKALREYGMVE
                     SQIDEFTDSTIANQQRLLANNYVELSREEIREIFANLY"
     gene            23120..24040
                     /locus_tag="KPD58_000668"
     CDS             23120..24040
                     /locus_tag="KPD58_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HBG0264455.1"
                     /translation="MYKSENFSKKSVLINSFLSKFLKIIGWGLVSLFTLSALVLSTIS
                     IIIGWAVILPIQVCAVFLLIGGVCIFKGKRLGDQISRYKKYCTIINNRNIIPVELIAE
                     NTSKSLNFIIKDVQKMIDKNYFINTYIDKRNNQIVLTNEDFVPPRYEDVAYEVKEEEK
                     TDESNEVDTIINKGMNYLTQIKEANKNIKSESMGDKIVQVEDVTSKIFDVVKHDPSKL
                     TQIQKFMDYYLPTTLKLLNSYHTLEEQGIDRENITTTMESIENTMNTIVVAFENQLDY
                     LFEDEAIDISTDITVLENMLVQEGLTSGKF"
     gene            24155..25312
                     /locus_tag="KPD58_000669"
     CDS             24155..25312
                     /locus_tag="KPD58_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBG0264456.1"
                     /translation="MNNLDDIPVMPTLTLDPFGEESNTNDIDNSDLLMKKDEKDPEEE
                     KLSESERKMVKEFAEKIDITNTNMILQYGAGAQKKVASFSETALKSVKTRDLGEVGDM
                     LTNLVTDLKAFSADEKEQSGFLGIFKKANNKISNLKTKYDSAEVNVDKVSKELQKHQV
                     KILKDIAMLDKMYELNLAYSKELTMYIIAGKQKLKDMKEYEMPKLREKARLSGSTEDA
                     QSVNDMVSLCDRFEKKIHDLELTRMVSLQMAPQIRLVQNSNNLMAEKIQSTIVNTIPL
                     WKNQIVLALGISHLNQAMKAQREVSNMTNELLLKNAETLKMGTIETAKESERGIVDID
                     TIKKTNQSLISTIDEVVKIQHDGRIKRQEAEVELSKIENELKSKLLEFTVK"
     gene            complement(25397..26896)
                     /locus_tag="KPD58_000670"
     CDS             complement(25397..26896)
                     /locus_tag="KPD58_000670"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBG0264457.1"
                     /translation="MAFYFDTPSHTFSEYLLVPGFSSTDCIPANVSLKTPVTKFKKGE
                     EADIYMNIPLTSAIMQSVSDDKMAIALAKEGGISFIYGSQTIENEAAMVARVKSHKAG
                     FVVSDSNIKPDNTLKDILDLKEKTGHSTVAVTEDGTSTGKLLGIVASRDYRISRMDLD
                     TKVSEFMTPMSSIVYANKDVTLKEANNIIWDHKLNSLPVLDDNGNLMYMVFRKDYSSH
                     KENPLELLDSSKRYVVGAGINTRDFAERVPALVEAGADVLCIDSSEGFSEWQKITLDF
                     IREKYGDTVKVGAGNVVDREGFLYLAEAGADFVKVGIGGGSICITREQKGIGRGQATS
                     IIEVAQARDEYFEKTGIYIPICSDGGIVYDHHITLALAMGADFIMLGRYFSRFDESPT
                     NKVNINGSYMKEYWGEGSARARNWQRYDLGGDKKLSFEEGVDSYVPYAGSLKDNVTLS
                     LSKVRSTMCNCGALSIPELQKNAKLTLVSSTSIVEGGAHDVMLKDNRNL"
     gene            27492..28916
                     /locus_tag="KPD58_000671"
     CDS             27492..28916
                     /locus_tag="KPD58_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HBG0264458.1"
                     /translation="MSSSNQSTIKTSIQRVGKFLSGMVMPNIGAFIAWGLITALFIPT
                     GWMPNEKLSTIGDPMIKYLLPLLIAYTGGKAIAGQRGGVIGAAAAMGVIVGADIPMFI
                     GAMIMGPFAGWVIKKFDKFVDGKIPTGFEMLVNNFSIGIIGMLLAILGFYAIGPAIVA
                     GTALIESGVQFIVSKSLLPLVSVFIEPGKVLFLNNAINHGILGPIGIAEAKEAGKSIM
                     FLLESNPGPGLGVLLAYWMFSRGSVKQSAPGAVIIHFFGGIHEIYFPYILMNPVLILA
                     TMAGGAAGILTFSILGAGLVAAPSPGSIFALMALAPKGGLLPVLAGVAVATVVSFLVA
                     APFVKRASANQSEEDSTSLEEAKAKMSDMKSASKNSEKNIEEKQLEVNEIKKIVFACD
                     AGMGSSAMGASRFKNRIKNLDLNIEITNSSVDNLPDDTQIVVTHNTLVERVAKNNSSV
                     EIVSINNFLNDPNLDTLFKRLESK"
     gene            28951..31080
                     /locus_tag="KPD58_000672"
     CDS             28951..31080
                     /locus_tag="KPD58_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBG0264459.1"
                     /translation="MKKKKITSRQKKIILMIVENSKKNIPITISEIAGTLELSSRTVL
                     RDMSGIEKWFDENDFNFVKKPGVGLILEENIENQNFIIELLEEEKIEKEYSKEERNLI
                     ILSKLLVSNEPVKSYYFAKILKVSEGVLNNDFALASKWLERFDIELVRKPGLGVYLKG
                     QEKSFREAYVNLIYDSFNEKEILDMVRNISENIQTDKAIEILSENRLLNLMDRCIIRK
                     VEATLTKKLSDLDVNLADSAYIGLVVHISLALQRIKNGENITMDKEFLKELSITEEFK
                     LAKEIVKGMETDFNMDIPVDEVGYITMHIRGAKQRSSSNHKALNLDDIEIMEITNKMI
                     DLAEDEFKISLKNDERLFKDLANHLGPSINRLNMGLEIRNPLLDEIKSKYSYAYDGVE
                     KISRIIKDKLNINSIPESEIGYIAMHFASAIEKNLMMNTNINIVVACPTGIGTSRFLS
                     TKIENKFPNLNILETISAINIDEEYLKEKDVDLIVSTVELNTSLNYICVGPFMSLDDE
                     QIIKEKIKSIAQNKLINLNAKNDTKSKNKVYEQITESMNIGKDILQFLEEIRFEKFES
                     KDLSELIEDSSKIFAKSSEDIISIKESLKERLKISIPYIEESKILLLHCMSERIDIMK
                     LSIIKLENRIVLDSNEEIDNVVFMLLPKNSPSYQRQIMSEISGSLIDNIIFTNKINKF
                     SIEEMALEVKDIVFNFYTNRLKAFIDK"
     gene            31110..31532
                     /locus_tag="KPD58_000673"
     CDS             31110..31532
                     /locus_tag="KPD58_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0264460.1"
                     /translation="MSKVLNENNIFLGLDSVSKEEAITLAGRKLVENGYVKEEYIPAM
                     LEREKVMTTYMGMGVAIPHGVNEAKKEILSSGIVILQFPNGIDFDGEKAYLLIGIAGV
                     GDEHLEILSNIAVVLDDDLTERLKNSNDKQAFMEAFAN"
     gene            31551..32702
                     /locus_tag="KPD58_000674"
     CDS             31551..32702
                     /locus_tag="KPD58_000674"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBG0264461.1"
                     /translation="MKKAIQFGAGNIGRGFIGGLLVKSGYHVVFADVNEGILNSINKD
                     KKYTIHIRDVECIDEVIDNISAVSSIKEEIIDEIVQAEIITTAVGPLVLTKIASTIAK
                     GIKARKEKGLTSNLNIIACENAIYASSSLKEEVLKYLNKEEVEYLEMYVGFPNCSVDR
                     IVPPGKNENPLDVTVENFYEWNVEKQGFKGEIPTIVGMNLADNLMAYIERKLFTLNTG
                     HAITAYIGYLKGYKTIEESIKDKFICDIVKSAMVESGEGLIKKYNFDSEVHYKYIDKI
                     LNRFKNPYLNDDVLRVGREPLRKLSDKDRLIKPLMTAKSYGLSVDNLILGIGAALHYN
                     NSEDTQSVELQELIKSIGVKKAVAKIANISNDEELLNNIEKSYIFMKNL"
BASE COUNT        12891 a         3676 c         6145 g        10027 t
ORIGIN      
        1 aaagaagaaa aaaattattg ggatgaatat atgcctttgt atgaagaatt aaattcatat
       61 ttttataatg ccattgtaaa ttcaaaattt aaatatgatt tgataaaaga gtttggagag
      121 caatttttca caatagtaga gtattcttta aaaagttttt ccaaagaaat aataagtgaa
      181 ttgcaggaag aaaataaatt gtgttcagag tatactagac ttttagcttc agctgagata
      241 atgtttgatg ggaaaataag aaatttatct ggtatgggga aattcatgta ttctaagaat
      301 agaaaaacaa gagaattagc aaacaaagca tattataatt tctttgaaga aaatgaaact
      361 aaatttgatg acatatttga taaattggtt aaacttagag ataagatgtc taaaaaatta
      421 ggttttgaag attttgtaga gcttgggtat gttagaatga tgagaagtga ctacagagaa
      481 tatatgataa aaaatgttag aaaacaagta ctaaaatatg ttgttccaat ggcaaatgag
      541 ctttatgaaa aacaagctaa aagaattggt ctagaatatt taagttacat agatgaaggc
      601 gtggaatttt taacaggaaa tgcaagttta aagggagatt caagatacat aattaaaaat
      661 ggtaaaagga tgtattctga actgtcaaaa gaaacaaatg aattttttga ttttatgtta
      721 gaaaatgaac ttatggattt agaaacgaag aaaggaaaag gagctggagg gtattgtact
      781 tatataccag attataagtc accattcatt ttttctaatt ttaatcaaac tgctgatgat
      841 atagatgtat taacacatga ggcaggacat gcatttcagt tgtatatgtc tagatggatt
      901 gatatgccag aaattaattt tccaacgctt gatagttgtg aaatccattc aatgagtatg
      961 gaatttataa catggccgtg gatggattta ttttttaaag aagatacaga taagtacaaa
     1021 ttcactcact tatcatcttc aattaaattt ataccttatg gagtaattgt tgatgaattt
     1081 caacactata tttacaaaaa tcctaatgta gataagtcaa aacgaaaaga aatctggaga
     1141 tttttagaga aaaaatactt acctcataga aaatatggtg ataattcttt tttagagaga
     1201 ggttgttggt ggtttaaaca aggacatata tttaaaaatc cgttttatta tatagattat
     1261 gtattagctc aaatttgtgc gttacaattt tggaaaaaaa tgattcaaga tagagatgca
     1321 ggatggaaag attatataaa tatctgtaaa gttggaggaa caaaatcatt tttagatatt
     1381 gtgagtatgg gaaatttgta ttctccattt gatgatggct gtattgagtc tattataggt
     1441 gatgttaaat cttggtttga tgaaataaat gacagcaaat tataaaaaat tatgagaatt
     1501 ttattgtata aaagtgaaaa attaaaaaat atataaaaag tgattatttg ttgtgaaaat
     1561 agtcttgttt ttaactaaat tgataaaata aactaaaaaa gatttgtact taaaaaaaaa
     1621 agaaaataca agaaatatgc aatatcaaca tttggaaaaa aaagtaaaaa tgtatacaaa
     1681 atacatataa aaaaactatt gcaaatttta tagaatgttt atatacttaa gtcaacagag
     1741 atagaaaaaa gctatttata gaaaacaaag gataaattgc ttaattttaa attttctgaa
     1801 aaaaataata atccggatga agagtatggg agactgcttt ataaaaagcg accgaaggga
     1861 caagtttaat cttacttacc caaaagatta gatgaaattc tcaggtaaag gtgactgtac
     1921 ttggacgggg ctctgaaaat gattttaaca gggaagacaa attgggagat ttgtttttcc
     1981 tgttttttat ttatcaagta ataaataaaa gtttgaaaga gagtaaaaaa atgataataa
     2041 ttacgaataa tccaaaagta aaagaagaag ttcaaggcag agaagttttg tttaaagaca
     2101 caacttatat tggaatctta gaagcaagta gagatttgat acatgaaggt tatgaacttt
     2161 tatctcatcc actctatggg agtgtaaagc caaatgaaac accttacagg actgttatac
     2221 taaagaaagg aaatcgtttg gatataaact ctttaacctt aattgaagaa gcaatcatca
     2281 ctgcaagtaa atttcagaat aataaaaaga ctccaaaatg gacagaaagt gttcaagatg
     2341 attttagagt aattgattat gatatatttt ataatacaat acaaagaatg cagtatgaat
     2401 aaaaaaaaat tgttaaaaag ttattaaaac agcctaaata ttaaatctaa tttaatatta
     2461 aataaaatga gaaatttaat tggggaggat aagtcaaaat ggaaaatgtg tatgacttag
     2521 taataattgg ttcaggacca gcagggcttg cagcaggtct ttatggagca agagcaaagt
     2581 taaaaactct aatcctagaa aaagacaaga ctggtggaca aattgtaata acacatgaaa
     2641 ttgcaaatta tccagggtca gttccaaatg caacaggacc aagtttaata gctagaatgg
     2701 ttgaacagtg taaagagttt ggagcagaaa tgctaagaga caacattgtt gatactgagc
     2761 tagatggaga tataaaagtt ttaaaagggg aaaaggcaga atatagagca aaagcagtaa
     2821 taataggaac aggagcaaca ccaagaaaga ttggttgtcc tggagaaaaa gaacttactg
     2881 gtaaaggggt ttcatattgt gcaacttgtg atgcagattt ctttgaagat tttgaagtat
     2941 ttgttgtagg tggaggagac agtgcattag aagaagcaat gtatttaact aaatttgcta
     3001 gaaaagtaac aattgtacat agaagacaag gatttagatg tgctaagagt gttgaagaaa
     3061 aagcaaaagc taatccaaag atagagtttt tattagatac agtaattgaa gaaatcaaag
     3121 gtgatggaat attagaatca gttgtattta aaaataaagt tactggagaa actcatgaat
     3181 actttgcaga tgaagaagat ggaacaatgg gagtatttgt gttcgttgga ttagatgctc
     3241 aaactgattt attcaaaggt aaagttgata tggatgaaaa aggttacata ataactgatg
     3301 aagatatgag aactaacata ccaggcgtat ttgcagcagg agattgtaga tctaagactc
     3361 taagacaggt tgtaactgca actaatgatg gagcaattgc atctatagta gctgagaaat
     3421 acattgatga aaaatttggg aactagaatc tgtaaattaa gaaatagtgt ctgtaaattt
     3481 aatgaataat atttaaaatt aaaattaata tttaaaataa aaataaaaat ttcaaggggg
     3541 aataagaaaa tgttagattt agataaggct acatttgaag aagaagtttt aaatgcagaa
     3601 ggttttgtat ttgttgattt ttggagtgaa ggttgtgagc cttgtaaagc tttaatgcca
     3661 gatgttcata aattagctga gacttatggg gataaaataa aattctgtaa gatggataca
     3721 acaaaagcta gaagattagc tataaaacaa aaagtattag gtcttccaac tatggctata
     3781 tacaaagatg gagaaaaagt agacgaagtt actaaggatg atgctacagt tcctaatata
     3841 gaaaacatga ttaaaaaata tctttaatat gttgataaat tattaacatt aaaagaaaaa
     3901 atctattttt atctgaagat aagaagaaaa aggtttttat gtccaacttt gttaaaaaaa
     3961 taattttaaa taaaataatt tttttacaat aaatattttt tagtaataaa ataaagattt
     4021 aaggcataaa aacacaataa tatatcttta aaaaaataag ataaattgta aaatgatata
     4081 attttgagaa aaactcaaat gcatatacac aaattaaata acaggaggtg caagtatgcg
     4141 tcttgaatta gggaaaatct ttataaaaga tattcaattt ggtgatgtaa cagaagtaaa
     4201 agatggagta ttattcatca ataagcaaga gatgctacaa gaaattggtg gagatgagca
     4261 tattaaatct atagatatag aattagctcg tccaggtgaa agcgtaagaa taacacctgt
     4321 aaaagatgtt attgagccaa gagtaaaagt tgaaggtaat gggggaatct tccctggtat
     4381 catgtctaaa gttgacactg taggagaagg aaaaactcat gctttaaaag gtgtagctgt
     4441 tgtaactact ggaaaaatag taggtttcca agaaggtata atagacatga ctggagaagg
     4501 agctaagtac actccatttt ctaaattaaa taatgtagta gtaatagctg aacctattga
     4561 tggtttaaaa caatacgctc atgaaaaagc tgttagaatg ataggattca aagcagcaat
     4621 gtacttagga gaagtagcaa gaaatcttac tccagatgaa gtttcagtat atgaaactaa
     4681 acctcttctt gaatcaataa aagaatatcc agagcttcca aaagtaggat atgtttacat
     4741 gcttcagact caaggtttat tacatgatac ttatgtatat ggagtagatg caaaacaaat
     4801 agtaccaact ttattatatc caacagaatt aatggacgga gcaattgtaa gtggtaactg
     4861 tgtttctgca tgtgacaaaa acccaagtta tgttcatata aacaatgggg ttgttgaaga
     4921 cttatatgca agacatggta aagatataaa ctttgtaggt gtaattataa ctaatgaaaa
     4981 tgtttatcta gcagataaag aaagatcttc aaactggact tctaaattat gtaaatattt
     5041 aggattagat gcagttatag tttcacaaga aggttttgga aatcctgata ctgaccttat
     5101 aatgaactgt aagaagatag aaatgcaagg tgttaaaaca gttatagtta cagatgaata
     5161 tgctggacgt gatggaggtt ctcaatcact tgcagatgca gatgtaagag ctgatgctgt
     5221 tgtaacaggt ggaaatgcca accaagttgt agttcttcct aagttagata aagttattgg
     5281 acatttagaa gtagtagatg taatagctgg tggttctgat ggaagtttaa gagctgatgg
     5341 aactattgaa gttgaaatcc aagctataac aggtgcaact aatgaaacag gatttggtca
     5401 tttaactgct aaaggatatt aagaagtatt ttattaaacg attgagtaat gttaataaaa
     5461 catattatag ataattataa taagtgttat agatatataa aaatatatta gaaatataat
     5521 aaggaggatt attatgagtt tacttagtaa taaaaaggtt cttataatag gtgaccgtga
     5581 tggtatacca ggacctgcga tagaagaatg tgtaaaaaca gtagaaggag cagaggttgt
     5641 tttctcatct acagaatgct ttgtctgaac agctgctggg gctatggact tagaaaatca
     5701 aaacagagtt aaagatgctg ctgataaatt cggagctgaa aatgttgtga ttttactagg
     5761 tgctgctgaa gccgaagctg caggtcttgc agccgaaaca gtaactgctg gagatccaac
     5821 tttcgctgga ccacttgctg gagttgcctt aggattaagt gtttaccacg ttgttgagga
     5881 accaataaaa tcattatttg atgaaagtgt atatgaggac caaataagta tgatggaaat
     5941 ggttttagaa gttgaagaaa tagaagaaga aatgtctggt ataagagaag aattttgtaa
     6001 attttaatgc ctaaaagcaa aaggggtgaa actaagagat gggaaaacta aaagcagtac
     6061 attatattaa tcagttcttt gctggtatag gtggagaaga aaaagctgat actaagccac
     6121 atgtggcaga aactttacct ccaataagtt tacaacttga taaattatta ggtgaagata
     6181 ttgaaatagt tggaacagta gtttgtggag atagctattt caatgaaaat atagatagtg
     6241 caagtgaaga agttttatca atggtaaaag gttttgaacc acaacttttc atagctggtc
     6301 ctgcatttaa tgctggtaga tatggggttg ctgctggaac aataactaaa gttgtaaaag
     6361 atgctttgaa tataccagct ttaacaggta tgtatgttga aaatccagga gcagatatgt
     6421 tcaaaaagga tgtttatgta gtagaaactt ctgattctgc agcaggtatg agaaaggcac
     6481 ttcctaagat agctaaactt gcagtaaaac ttgctaatgg agaagaaatt ggaactccta
     6541 aagatgaagg atacatagct agaggaataa gagttaacta cttccatgaa gacagaggtt
     6601 ctaagagagc tgttgacatg ttagttaaga aaataaaagg tgaaccattt gaaactgaat
     6661 acccaatgcc aaactttgat agagttgacc caagtaaagc tgtaaaagat ttatcaaaat
     6721 gtaaaatagc tttagttact tctggtggta tagttccaaa aggaaatcca gatagaatag
     6781 agtcttcttc agcttctaaa tatggaactt attctatagc aggagttatg gatttaacag
     6841 aagagactta tgaaactgca catggtggat atgacccagt gtatgcaaat ttagatgcag
     6901 atagagtatt acctgttgat gttcttagag atttagaaaa agaaggtgta attggaaaat
     6961 tacatgagac tttctataca actgtaggta atggtacatc tgttgcaaac tctaaaaaat
     7021 atgcttctga aattggagca gcattagtgg ctgatggtgt agatgcagta atattaactt
     7081 ctacatgagg tacttgtact cgttgcggtg caacgatggt aaaagaaata gaaaaaactg
     7141 gacttccagt agttcatatg tgtacagtag tacctatttc attaacagta ggggctaata
     7201 gaatagttcc aactatagca atacctcatc cacttggaaa tccagcgctt gaccctacag
     7261 aagaaaaagc cctaagaaga ggccttgtag aaaaagcatt aaatgcttta actacagaag
     7321 tagatggtca aacagtattt gaaaaataaa aataacacac acatatatta tatagataaa
     7381 aatataaaca cacactaaaa ataaaaaata actatacaca aaaaaataat atagatttca
     7441 tgaatggata aattggagtt attcattcat gaaatcttaa aaaatgccaa aatacataaa
     7501 caataataga atcaaatata caataaaata caatgattag agcttttaat aagtttaata
     7561 agattaaaaa ataagtgttc ataaaattta tggaggtgca caaaatgact tatccagttt
     7621 taaaaggggc aggctatgta cttattcata ctccagatat gatagtgcaa aatggaagta
     7681 cttgtacagt tgaaagagca acaaatccag attcagaatt tttaaaagaa gtaagtaatc
     7741 atataagaag ttatgaagat gtagttaact acatgccaaa tcaagtttat ataggaaata
     7801 gaagaccaga agaattaaga gatttaccaa tgccatggtg tgaacaaaag attgagggaa
     7861 ctagaaatgg taagtttgga gaaataatgc ctcaagatga gttcatagct ttaatgcaaa
     7921 taagtgatgc atttgattta gtaaaattat cacaagaatt tattgatgaa gtaaagccaa
     7981 aaatcgaaaa taactaccct gaaattgcac catttgtagg taaattaaag ggtgatgata
     8041 tagaagaagg taaagaatta gtagcaacac acatagctga aggattatac catgatggaa
     8101 aatttgttgg ttatgtaaaa agagcacatg atgtagatgt taacttaaat gcccatacaa
     8161 tgtttgaaaa tctagtagta aaagcatctg gagttttatc tgctattcaa atgctaagac
     8221 atagcaaaat agaccctgct gaaatagatt atgttattga gtgttcagaa gaagcttgtg
     8281 gagatataaa ccaaagaggt ggaggaaact ttgctaaatc aatagctgag atagcaggac
     8341 ttcaaaatgc aactggttct gacacaagag gattttgtgc agcaccaact catgcattaa
     8401 ttcaagcagc agctttagta aaagctggca tacataaaaa tgtaatggta gtagcaggtg
     8461 gagcaagtgc taaacttgga atgaatgcta aagaccatgt taaaaaaggt cttcctgtac
     8521 ttgaagatgt tgttggagga tttgcagtac ttgtatctga aaatgatggt gtaaatccag
     8581 ttataagaac tgatttaaca ggtaagcata ctgttggaac tggttcttca ccacaagcag
     8641 ttatgacagc acttataact tcaggtcttg atagagctaa tttaaaaata acagatgttg
     8701 atgtgtattc agttgaaatg caaaacccag atataactaa gccagcagga gcaggagatg
     8761 ttccagaagc aaactacaaa atgatagggg cacttgctgt taaacgtgga gacttagaaa
     8821 agaaagaatt gaaagatttt gtttctaaca aaggattacc aggatgggca ccaactcaag
     8881 gacatatacc atcaggagct ccttatatag gatttttaat agatgattta actacaggaa
     8941 atagaaatag agctatgata gttggaaaag gtagtttatt cttaggaaga atgactaact
     9001 tatttgatgg agtatctttt atagctgaaa gaaatacagg tgttactgaa gagacttcag
     9061 gaatatctaa ggatgaaatt aagaagataa tagctgaatc tatgaaaaaa ctagcattag
     9121 atatgttaga agaatagggg tgaggatatg tcaaaaaaag taatcgcaga tgtattctta
     9181 gaagtagcta atgcaataga aagcggcgaa tttggcaaaa aagtaaaaat tggtgttact
     9241 acacttggaa gtgaacatgg tgtagaaaac atggttaatg gtgcacaatt agcaaaatct
     9301 aatttatttg atattgtgct tattggacca aaagtggaaa ctgaccttga agtagttgaa
     9361 gttaatgatg aaaaagaaat gcatgcaaaa atggaagaat tattagattc aggatatatt
     9421 gatgcttgtg ttactatgca ttataatttt ccaataggtg tatctacagt aggtagagta
     9481 ataactccag caaaaggaaa agaaatgata cttgcaacaa caacaggaac aagtgctact
     9541 aatagaatag aggcaatggt aagaaatgcc atttatggta ttgctacagc taaatcaatg
     9601 ggtaacaaat gcccaaaagt tggaatactt aatgttgatg gagcaagaca agttgagaag
     9661 tgcttaaaag aattaaaaga taatggatat gacatggagt ttgcggattc aattagagca
     9721 gatggtggat gtgtaatgag aggtaatgat ttacttgtag gtgcacctga tgttatggtt
     9781 actgatacat tatctggaaa tatatttatg aaagtatttt cttcatatac tacaggtgga
     9841 gattatgaag ctcaaggctt tggatacgga cctggagttg gagaggacta tgatagaaag
     9901 gtacttatag tatcaagagc atcaggttct ccggtagtag caaatgcttt aaaatacgct
     9961 tatgatgttg taaagggaga tataagtaat gttgcaagaa atgaatttgc aaaggttaaa
    10021 aaagcaaaat ttgatgacat tatatcctct cttactaaaa aagaagttaa agctgaaaaa
    10081 gtagaagtta aaatgccaga taaagaaatt gttactcgtc aaatagctgg tgttgatata
    10141 atggatttag aagatgcagt atctgagtta tggaaaaacg gaatctatgc tgaaagtggt
    10201 atgggttgta caggtccaat agttttagta aatgaagcta aaggagactt agcagttgaa
    10261 actttagtta aagctggata tactgcaaaa taagtacatt ttttatatgt aaataagaac
    10321 atatagttat aattttattg attataatta tatgttcttt ttattttata tcttgtcaat
    10381 atttatatat taacacaatc catttatttg attctctagc taatttatta tatttattca
    10441 gttaaaatta agctttctaa ctaaataaaa actaatgaag tattaaaatg aaattgataa
    10501 aatatgaaaa aattttagga ataaagtatc tgttagataa aaataagatt aattagtgat
    10561 ataatataat taagataaaa tttattattt ggaggcaaaa tcatgagcag agtaaaaaat
    10621 gtagtagaat tgttggaaac taaaggtgtt gatgcactat atctaacaaa gaaaactaat
    10681 gtaaactata taagtggttt tcctgatgaa gaagcatatg cagtgatttg caaagatgga
    10741 aactttttag ttactgatag tagatatatg gagcttgcag aaaaggtttg caaagatttt
    10801 gaaattataa attggcataa ttttgataga agtgtggcaa aggcagttaa aagtgtatgt
    10861 gataaagtgg gtataaagaa acttgggttt gaaagaacta atatagtgtt tgataaatat
    10921 gaagaactta aaaatctgat tgagaaggat aatggagaat taatacctac agaaaatatt
    10981 gtagagactt tgagatatgt aaaagacaaa gatgaaatca aaaatacaag aaaagcttgt
    11041 gaaatagcgg ataaggcatt agaagaattg attccacata taaaagctgg agttagcgaa
    11101 attgaactgg ctacaaagtt agagtacttt atgaaaatga acggagcaca aaatataggg
    11161 tttgaaacta tactgatatc aggagcaaag acttcattgc ttcatggtaa gcctagtgat
    11221 aagattatag aaaaaggtga ttttgtactt atagactatg gcgctatgta taatggctat
    11281 atatctgata caactagaac ttttatagtt ggaggagctt ctgaaaagca attagaaata
    11341 tataatttag ttaaggaagc tcaaaatgta ggggttgaga acatgaaagc tggtgtacat
    11401 gcgacaattc cagatgctga gataagaaaa gttgtcaaaa aatatgaaga ctattactat
    11461 caaggtatag gacatggagt cggtagagat gttcatgaag aacctttcat tggtaattat
    11521 ggtgataaaa tcatagaaga gggatgtata ataacaatgg aaccaggtat ttatttccct
    11581 ggatggggtg gagttagaat agaagatact gttttgatta ctaaaaatgg tccagaaaga
    11641 cttactaaat tccctaaaga tttgatgatt ttagacaaat aatttaattt atatatattt
    11701 ttttacataa aatataacat aatctcacta ttaagctcta tttacatatt atagtaaaga
    11761 gggcttaatt ttattgtgag aggtgatagt atatatgtat aaaaaacata aatttataaa
    11821 aaatataaaa aaagcctggt ataaaatcaa attaattgat agatgcctta taattgtaat
    11881 ggcaatatta atgtttcaat ctatatataa tttatttgtt aatgaagtaa attcacaaga
    11941 tactacaact attgatgtag tagtaagaac cacttcagca gctatatttg ggtatttttt
    12001 aagtgctaat tttataaaga gacctttaag gaagtataca aatactgatg tatcttcaaa
    12061 tctttttata gataataatt tatccccaaa aggtcaagag tctaatcaaa ataatataat
    12121 gaatgtcaaa aataccattg gatttacttc agaagctgat aattatgaaa agaaaatacc
    12181 tattaataat aatgaaggtt ttgaagaagg agaaactagt gaattgcaaa ttattatagc
    12241 tactgtaatt tgtatagtag cattaacagt actttttgtt gtaagaaatt ttacaactac
    12301 aacatcagct tctttaggta ctatttcaca gatgagagat tttgtctcag gatgcgtggg
    12361 ttttttgcta ggttgtccaa gtaaacctga ttaaaaaagt attatcaata aaaggtattt
    12421 taaaaagtat cagtataata atatttagat ttaaatgtat acaattataa ttctaaacat
    12481 tctatattgt atttttatac tgatactcta aactatacta tttattaaaa tattttaaca
    12541 ttgtatttaa acaaaatatt atatctgtat aatataaatt tagtttatat gaatagatgc
    12601 catatactca ataaaaagtt tcatactatg agatatgtat tgatttcttc tccaaagaac
    12661 cccaatattc gcagtaattg cacgttctaa tggtattgaa cagagatttt ctgtattttg
    12721 aaatatttct ttatacaata ttgttgaagc caagtcattt tctagtaaat aacgtatggt
    12781 agaaagttga tttgagtgta aaattatatt tggcttagct ttttcaagtc tttttgtaat
    12841 aatcttatag tgataagaac cttcagataa gattacaaaa ggtacatttt ttaacatata
    12901 attcgatata atgggatgtt ttgccaattc atttttggga tttgtaacta agtataattc
    12961 tgttttatag attgtctttg aatctatatc atcataaata tcactgtcaa ttataccaat
    13021 acaaaaatca agtgtagatt cttcaatcat tttagctcca acaaatgttg gaacttcaaa
    13081 aattttaagc tctatatctg gataaattac attaaatcta ggaagtattt ttgaaagcaa
    13141 aaacgtacct aaaactgtag gaatacctat tttgagagaa gcttttctct tttttcctaa
    13201 atcaagtgcg tcacattgaa aatcctcaaa ttgttttata aaatgttttg acttgtctaa
    13261 aaaaattttt ccttcaggcg ttaactctat tttattacct attctgtaaa aaagttcaaa
    13321 gccaagttct ttttctaatt ctttaattgc taatgttatg gatggttgag aaatatgaat
    13381 actttccgca gccttagtca tgtttttata tttacagaca gtttgaaaat agtataaata
    13441 gtttatattc atttggtatt agttcctttc atttacatat tagtgacaag tatataatta
    13501 acaatagcat aagttttgta tattgttgta aaaaaataga atagttttaa actatcatag
    13561 cataagtttt tgatattttc aatctattaa gaatagtgct attattaaat taacaaaaga
    13621 gaatagctgg tatggtttag agtaaatcaa tctatattac agttatatca acgaatatca
    13681 aaaaattatc tattagaaaa taaaaattat ttttacaatt aaattgttaa ttaaatcata
    13741 aagaaaaaag tgatattaac aagataaaat ttttttaatc agaaatgtta atttaataac
    13801 aaaatagagt attggggaga aaggagaaat atatttttta ataatatttg ttaatttaat
    13861 aacgaaaaat gaaagggagt gatttagtga agtttgaatt gatggatttt ttaatgaatc
    13921 cttttgtcct tatgtttgct gcagtaatta caggaatttt atttggaaaa attaaatttg
    13981 ggaagtttaa ttttggggta tcaggagcat tatttacagg attatttatt ggatggcttg
    14041 catatagctt ggggaatcta ataatagcaa aaggggaaac agcagcagga tataaggcag
    14101 caactgtaat gatgggtaat ggaattattt cttctgactt ctttgatttc tttttaataa
    14161 tatttgttgc agcagttggt ctattggcag caaaagatat gaaagctgta ttaaagaagt
    14221 atggagcaag atttgtaatt ttaggagttc ttataacttt tataggtggc tctatgactt
    14281 atgcaatgac tttactatca agtgataaag gaagtagtgc ttatgaagtt tctggcgttt
    14341 acacaggagc attaacaagt tcgcctggat tagctgctgc tttagaaaca gctggtaaac
    14401 atgcagaaga tgtatcaaaa gaatttgaaa aagcttcaat aaaagataaa aaagaaattt
    14461 taaaagtagt cgacccagaa ggcaaattgg atgtgaatac aactacatca ctgactcaag
    14521 agcaaataga taagtatatt gcatatgctg aagctggtgt tggtataggt catgcagtcg
    14581 catatccttt tggagtttta atagtaatct taggtgtaaa tttccttcca aaattattta
    14641 ggatggacct aaaagaagaa agaagaaaat atgagaagga aatgaaggaa gcaagagata
    14701 gtgtttctgg taaaacgata ccagaagttc catttaatat aataacattt ttcttgacat
    14761 gtttagctgg atacttagtt ggaggaatac atgtatttat gggaccacta gggtacttca
    14821 cactaggtgc aacaggaggt tcactaattg tatctctagt tcttggatat ataggtaaga
    14881 taggggtagt aaatttcaga atggaagaaa aggttttaaa tattttaaaa caaatagggc
    14941 tagtattttt cttagcaata gttggattaa gatatggtgg aaaagtagtt gattcaatta
    15001 tgacatctgg tatgcaccta gccttagtag caatagcagt aggtgttaca gctatgatga
    15061 taggattctt agttggcaaa tacgtattta aattaaattg gattttatta tctggagctg
    15121 tctgtggtgg tatgacatca acaccaggtc ttggagctgc tgttgatgct cttgatagtg
    15181 atgacccagc agcaggttat ggtgctacgt atccatttgc attattaact aaagttatat
    15241 tggttatagt tttacacaaa cttccaatgt aaataagatt ttatatgggg ggatatttat
    15301 gaataatgaa ggagcatttg aagaattaag tggaagatta agaaataaag aattgtgtga
    15361 tttaataatg aatgcagatg aagctgctaa aattatagag gatggcatgg ttattggagt
    15421 tagtggattt acaccttcag gctatccaaa agcagtacca ttagcagttt cagaaagagc
    15481 taaaagtgga gaaaagataa aattaaccgt atattcagga gcatctttag gtccagaagt
    15541 tgatggagca tggtcagaag caggtataat tgaaagaaga cttccttatc aaactaattc
    15601 aatacttaga aataatatta ataaaggtgt tgttgattat atagatatgc acctaagtca
    15661 ttctactcaa tttttaaatt atggaactat acctaaagta gatgtagcta tagtagaagc
    15721 acttgcaata actgaagaag gaaatattat accaactagt gggataggta attctccatc
    15781 ttttataaaa agtgcagata aagtaatagt agagataaat cttgcaaaac caatggaaat
    15841 ggaaggtatg gcagatatat atattacgga aaatccacca aatagaaaac caatagaaat
    15901 aaatcatcct aaggatagaa ttggaacaac atatatacct tgtggtttgg ataaaattgc
    15961 aggagtagta attaccaata tgcaagataa gacccgtcca ttgggagttg tagatgaagc
    16021 ttcgaaaaaa atatcaaata atattattgc ttttttaaga gaagaagtaa aggcaggcag
    16081 attatcaaag aatttacttc ctcttcaatc tggtgtagga agtgtagcaa atgcagtatt
    16141 atatggatta tgtgaatctg aatttgaaaa tttaacttgt tatacagagg ttgtacagga
    16201 ctctatgttg gatttaataa gaatgggtaa agttacaatg gcatctacta catctgtaag
    16261 tccatctcca gaaggattaa taaaatttga aaaagatatt gattttttta aggataagat
    16321 tatattaaga cctcaagaaa ttagtaacaa tccagaaata gccagaagaa ttggtgtaat
    16381 tgcaatgaac actgcaattg aagttgatat ttatggaaat gtaaattcta ctcacataat
    16441 ggggtcaaag atgatgaatg gaattggtgg ttctggtgat tttgctagaa atggagcaat
    16501 aactatattt agtactgagt caattgcaaa aaatggtgat atatcatcaa tagtacctat
    16561 ggtatctcac gtagaccaca ctgaacatga tgtaatggta attgtaacag aacaaggata
    16621 tgctgattta agaggtcttg cacctagaga aagagctata aaaataatag aaaattgtgc
    16681 ccatccagat tataaagaac aacttaggga ttatttaaat agagcttgta aaagtggagc
    16741 aaaacaaaca cctcacatat tggacgaagc tttatcatgg catagtaaat ttatgagtac
    16801 aggtacaatg aaaaaggctg aaacttttaa aagtgcatta taaaatattt aataaattgg
    16861 aggtagattt taaatggaaa aagcagtgga aaactttgaa gatttaagta aggaatacat
    16921 taatggttat attgagagag caagaaaagc tcaaagagag tttgaatgtt atactcaaga
    16981 acaagtagat aaaatagtaa aaatagttgg aaaagtagtg tattataatg ctgaatattt
    17041 agctaaactt gctgtagaag agactggaat gggagtatat gaagataagg tagctaaaaa
    17101 taaaagcaaa gcaaaagtta tatataacaa tttaaaagat aaaaaatcag ttggtataat
    17161 tgatatagat agagaaactg gaataacaaa ggtagctaaa ccagttggag tagtagcggc
    17221 aataactcct tgtacaaatc caatcgttac acctatgagc aatgcaatgt ttgcacttaa
    17281 gggaagaaat gcaattatta tcacacctca tcataaagca attggatgta gtacaaaaac
    17341 tgtagagatg attaatgaag aattagaaaa aataggagct ccagaaaatt taatacaaat
    17401 acttgaccaa caatcaagag aaaatacaag aaatttaatt tcatcagcag atgtagtaat
    17461 tgccactgga ggaatgggaa tggtaaaagc tgcgtactca agtggaaaac cagcacttgg
    17521 tgtaggggca ggaaatgttc aatgtatcat agatagagat gtagacatta aagaagcagt
    17581 acctaaaatc atagcaggaa gaatttttga taatggtatc atttgttcag gtgaacagtc
    17641 agtaatagta gcagaagaaa tgtttgataa aataatggat gaatttaaaa ataataaagg
    17701 ttttatagtt agagataaag tacaaaaaga agctttcaga aatgctatgt ttgtaaataa
    17761 atctatgaat aaagatgcag taggtcaatc agttcataca attgcaaaaa ttgcaggtgt
    17821 tgagatacca gaagatacaa aaataatagt aatagaagct gatggaccag gagaagagga
    17881 tattattgca aaagaaaaaa tgtgtccagt tatatctgca tacaagtata aaagttttga
    17941 agaaggtgta gctattgcaa aagcaaattt aaatgtagaa ggaaaaggac atagtgtatc
    18001 tatacattca aatacagtta aaaatataga atatgcagga gaaaatatag aagtatcaag
    18061 atttgttatt aatcaatgtt gtgctactag tgctggagga agtttcttta atggtcttgc
    18121 acctactaat actctaggtt gtggttcttg gggaaataac agcatctctg aaaatcttga
    18181 ttataagcat ctaattaata tttctagaat tgcttattat atgccagaaa atgaagtccc
    18241 tacagatgaa gaactttggg gataaaattt agacaaaaaa tattcattct aaattaaagt
    18301 aggaatatat tttaatttag aatgaattta agacataaat taaatttaaa ttagtttatt
    18361 ggactaaatt taaaatatta agagacatat agaagaagga agtattataa tttaaaataa
    18421 ataaaaaaat ctataaaaga gaaggagtgg ttttattatg gcattaatga caggagctca
    18481 atatattgaa agtttgagaa aattaaatac taaggtttat atgtttggtg aagaggttaa
    18541 gaattgggtt gaccatccta tgattagacc atctataaat tgtgtagcag caacttatga
    18601 cttagctcat gacccagaat atgcagattt aatgacagtt acttcaaata tcacaggtga
    18661 aaaaataaat agatttggtc atcttcatca aagtgttgat gatttgataa aaaaagtaaa
    18721 aatgcaaaga ctatgtggtc aaaaaacagc ttcttgtttc caaagatgtg ttggtatgga
    18781 tgcgtttaat gcagtgtatt caacaacatt tgagtgtgat aaagcccatg gaactaatta
    18841 tcatgataat tttgtaaaat acttaactta tatacaagaa aacgatttag tagttgatgg
    18901 agctatgact gaccctaagg gagatagaag tctatcacct agtgctcaac ctgacccaga
    18961 tatgttcctt catatagtag aaagaagaga agatgggata attgtaagag gagcaaaggc
    19021 acatcaaact ggttcaataa attcacatga gcaccttata atgcctacta taagtatgac
    19081 tgaagcagat aaagactacg ctgtatcatt tgcagttcct tcagatgctg aaggagtgtt
    19141 tatgatttat ggaagacaat cttgtgacac tagaaaatta gaagaaggtg cagatgttga
    19201 tttaggaaat aaggagtttg gaggtcaaga agctttagta gtatttgata atgtatttgt
    19261 tccaaatgat agaattttct tatgtggaga atgggatttc tctggtatgt tagtagagag
    19321 atttgcagga taccatagac aaagttatgg tggatgtaaa gtaggagtag gtgatgtaat
    19381 aataggagca gcagctttag cagctgatta taatggagca aacaaagctt cacatataaa
    19441 agataaacta atagaaatga ctcaccttaa tgaaagctta tattgctgtg gtatagcatg
    19501 ttcatcagaa ggtcacaaaa cagaagctgg aaactatcaa atagatttac ttcttgcaaa
    19561 tgtgtgtaaa caaaatgtaa ctagatttcc ttatgaaata gtaagacttg cagaagatat
    19621 agctggaggg cttatggtta ctatgccttc agaaaaagac tttaaatcag accttaaagt
    19681 tggaacaagt ggaatgacta ttggcgaagt atgtaacaag tattttaaag cttcttcagt
    19741 ggcttcaaca gaagaaagaa tgagaatatt aagattctta gaaaatatat gccttggttc
    19801 atctgctgtt ggttatagaa ctgagtcaat gcatggggca ggttcaccac aagctcaaag
    19861 aataatgatt tcaagacaag gaaacataaa tcaaaagaaa gaattagcta agaaaattgc
    19921 gggaataaaa aaagaggaag ctttaaattt ataaaaagtt gatatataaa gggaaattat
    19981 atattaatgt gtattaatta atgaaaaaat atgtagtata agagatagtt ataaaattat
    20041 ggtggttttt agctaagtaa agaaattttt aataaggaga atttacacat gacctgtgga
    20101 gttagatttt gtgggggctg taaccctaga tacgaaagag ggaaggctct tacaataatc
    20161 aaagagcact tcaatggtag ggtagatttt agtatagcac aagagaacgt cgagtatgat
    20221 ttgctactta taattggagg atgcgcaact ctatgcgcat cctatcaaca atataaagtg
    20281 aaaaatgaat gtgttaagat gtgggatttt agccatatag aaagagttat aaatgtgatt
    20341 gaatcagcaa tttgtgaaac ttaaaaaagc taaatggggg gatttcattg agttggcaag
    20401 aattgtatca aagtaaatta tgtagtgcta cagaagctgt aaaacagatt aaaaacggtg
    20461 atactgtagt atttgcacat tgtgtaggtg aacctcctgc actagtagag gctatgattg
    20521 aaaatgctga acaatataaa gatgttgaga ttaaacatat ggtaagcttg ggtagtggtg
    20581 gatatactgc taagggaatg gaagctcatt ttagagtaaa tccaatgttt gtaagtggaa
    20641 atgtaagaaa ggcaattgaa aatggagatg gtgattttac acctgcattc ttccatgaag
    20701 taccaaagtt gcttagagaa aaaagattaa aatgtgatgt tgttttagca caggtaactc
    20761 caccagatga acatgggtat tgtagcttag gaacatcagt tgattatact tatgaagcca
    20821 ttaaaactgc tagaacagta attgttcaag tgaatgacca gtttcctaga acctatggtg
    20881 aggtagtcca tgtatcagag tttgactata tagtggaaaa atcacaacct ttatttgaac
    20941 tacaacctgc aaagattgga gaagtagaag aagcaattgg taaaaattgt gcttcattaa
    21001 ttgaagatgg ttctacttta cagctaggga ttggtgggat accagatgca gttatgttat
    21061 ttttaactga taaaaaggat ttagggattc attcagaaat gatttcagat gggactttgg
    21121 ccctttatga aaaaggtgtt ataaatggta aatataaaaa ttttgataaa gaaaaaatga
    21181 cagttacctt cttaatgggt actaaaaaat tatatgactt tgcaaataat aaccctgcag
    21241 tagaggtaaa accagtagac tatgtgaatc atccagcaat tataatgaaa caacataaga
    21301 tggtttctat aaattctgct atacaagttg atttaatggg acaggtagtt gcagaggcta
    21361 tgggacttag acaatttagt ggtgttggag gtcaagttga ctttatacgt ggagtgtcta
    21421 tgggtgaaga tggaaaggca attatagcta tgccttcaat aactacgaaa aaagatggta
    21481 cagtaatttc aaaaatcgtg tctattgtgg atgaaggtgc acctataacg acatcaagaa
    21541 atgatgttga ctatattgta acagaatacg gtatagcaga attaaaagga aaatctctta
    21601 gagaaagagc gagaaattta ataaatatag ctcatccaag tgtaagagaa agtctagcag
    21661 tagaatttga aaagagattt aaggagaaat attaataata gaggggtgtt aataatggaa
    21721 gcacttagag tagttccaaa gatattttac tttgatacat ttaaagaatt taatgaagaa
    21781 tttaaaatag gaaaaaatga tttagtaatt actaatgagt ttatatatga gccatatatg
    21841 aaacctcttg gaatagatac aaatttaatt tttcaagaaa agtttggaac aggtgaacca
    21901 tctgatgaaa tgatagactc catgacaaag gaaatgaaga aatacaattt tgatagaatt
    21961 attgcatttg gaggaggaac aatagttgat atatgtaaga tattagcact tgatgttcca
    22021 gaaaagtcta ttgatttatt tgaaggagaa gtatcaccta aaaaagtaaa agagttagta
    22081 gtagttccta caacatgtgg tacaggtagt gaagtaacaa atgtagctat tgcagagtta
    22141 aaatctaaac atactaaaaa ggggcttgca gtagaagaaa catatgcaga ctatgcagtg
    22201 ttaattccag agacagtaaa agggttacct tacaaattct ttgttacaag ttcggtagat
    22261 gctcttatac atgctattga gtcttatctt tctccaaaag caagtccatt tacagagatg
    22321 tattctctac aagcaataaa aatgattatg gatgggtata agaagatagt tgataaagga
    22381 gaagaagaga gatttaatca tcttagagac tttgtacttg catcaaatta tgcaggaatt
    22441 gcttttggaa atgctggttg tgcagcagtt catgcactat cttattcaat aggtggagct
    22501 ttccatgttg cacatggtga agcaaattat cagttcttta ctgaagtatt taagatgtat
    22561 tctagaaaaa aacctaatgg aaaaattata aaatgtacaa aaattttagc agatgccctt
    22621 gaatgtgacc ctaactgtga tgtttatgga gaattagaaa agtttttaaa taaattaata
    22681 gctaaaaaag ctttgagaga atatggaatg gtggaaagtc agatagatga atttacagat
    22741 agtacaatag caaatcaaca acgactactt gctaataatt atgtagagct tagtagagag
    22801 gaaataagag agatatttgc aaatctttat taatacaatt ttttatttat atgtatagtc
    22861 aacataaaca atgctaatta tatttatgta caaataaaaa taagggaact taaattttaa
    22921 ttaaagttaa gttctcttat ttttatatgg agttaaatat aagtatattt ttaatttaaa
    22981 atatgcttat atttaagtct attactttat ttgaaacaat ttgtagaatt tcaaattcct
    23041 tgacagtaat aagtaagtaa ttgtataatc aaactgcaat atattaataa tattagtgaa
    23101 aatgaaagga ttataaaata tgtataaatc agaaaatttt tcaaaaaaat ctgtattaat
    23161 aaattcattt ttaagtaaat ttttaaaaat aattggatgg ggtttggtaa gcttatttac
    23221 attatcagcc ttagtacttt caactatttc cattataatt ggatgggctg taatacttcc
    23281 tattcaagta tgtgcagtat ttttacttat aggaggagtt tgtattttta aaggtaagag
    23341 attaggagac caaatttctc gttataaaaa atattgtaca attataaaca atagaaatat
    23401 tattccagta gaacttattg cagaaaacac atctaaatca ttaaatttta taataaaaga
    23461 tgtacaaaag atgatagaca aaaactactt tataaatact tatattgata aaagaaataa
    23521 tcagattgtg ttaacaaatg aagattttgt tccaccaaga tatgaagatg tagcatatga
    23581 agtcaaagaa gaagaaaaga ctgatgaatc caatgaagtt gatacaatta taaataaagg
    23641 aatgaattat ctaacacaaa taaaagaagc taataaaaat attaaatcag aatcaatggg
    23701 cgataaaatt gtacaagtag aagatgttac aagtaaaata tttgatgttg taaaacatga
    23761 cccatcaaaa cttactcaga tacaaaaatt tatggattat tatcttccaa ccactttaaa
    23821 actattaaat tcatatcata cattggaaga acaaggaatt gatagagaaa atataacaac
    23881 aactatggaa agtattgaaa atacaatgaa tacgattgta gtagcttttg aaaatcaatt
    23941 ggactatctg tttgaagatg aagccattga tatttctaca gatattactg ttttagagaa
    24001 tatgttagtc caagaaggat taacgagtgg taaattttaa cttcaataga aaaaatattc
    24061 tgttattttt aaacaattaa ttagtcataa agtataaaat gtgtattata taagttaatc
    24121 agtactttat gaaggaagag gagagaaaga tacaatgaat aatttagatg atataccagt
    24181 tatgccaact ttgacacttg acccatttgg agaagaatca aacactaatg atatagataa
    24241 ttctgactta ttaatgaaaa aggatgaaaa agacccagaa gaagaaaaac tgtctgaatc
    24301 tgaacgcaag atggtaaagg aatttgcgga aaaaatagat attacaaata caaatatgat
    24361 tttgcaatat ggagctggag ctcaaaaaaa ggtagcaagt ttttctgaaa cagctttaaa
    24421 gagtgtaaaa acaagagatt taggagaagt tggagatatg cttacaaatc tagttactga
    24481 tttaaaagca ttttcagcag atgaaaaaga acaaagtggt tttttaggaa tttttaaaaa
    24541 ggcaaataat aaaatttcaa atttaaaaac taagtatgat agtgcagagg taaatgtgga
    24601 taaagtaagc aaagagcttc aaaagcatca ggttaaaata ttgaaagata tagcaatgct
    24661 tgataagatg tatgaattga acttagctta ctcaaaagag cttactatgt atattatagc
    24721 aggaaaacaa aaattaaagg atatgaaaga atatgaaatg ccaaagttga gagaaaaagc
    24781 tcgtttatct ggttcaacag aagatgctca atctgtaaat gatatggttt cactttgtga
    24841 cagatttgaa aagaagattc atgacttaga attaactaga atggtatcgc ttcaaatggc
    24901 tcctcagata agattggttc aaaatagtaa taatctgatg gcagaaaaga ttcaatcaac
    24961 tatagtaaat accattccat tatggaaaaa tcaaatagtg cttgctcttg gaatttctca
    25021 cttaaatcaa gctatgaaag cacagcgtga agttagtaat atgacaaatg agcttttatt
    25081 aaagaatgca gaaactttga agatgggaac aatagaaaca gcaaaagaat cagaaagagg
    25141 aattgtagat atagacacaa ttaagaaaac taatcaatct ctaatatcta ctattgatga
    25201 ggtagttaaa atacaacatg atggtagaat aaagagacaa gaagcagaag ttgaattatc
    25261 aaaaatagaa aatgaactta aaagtaagct tcttgaattt acagttaaat aaaaattata
    25321 ctaatataaa aagagactgt ctcaaaatag agataagtct ctttttcatt gtttaaatga
    25381 ttaaagtata tttatattat aaatttctat tatcttttaa cataacatca tgagctccac
    25441 cttcaactat actagtagaa gatacaagtg taagtttagc atttttttga agttcaggaa
    25501 tgctaagtgc tccacagtta cacatagtag aacgaacttt acttaaagaa agagttacat
    25561 tgtctttcaa agaaccagca taaggcacat aagagtcaac accttcttca aaagaaagtt
    25621 tcttatctcc accaaggtca tatctttgcc agtttctagc acgagcagaa ccttctcccc
    25681 aatactcttt catataacta ccatttatat taactttgtt agttggactc tcatcaaaac
    25741 gagagaagta tctaccaagc attataaaat ctgcacccat agctaaagct aaagttatat
    25801 gatggtcata aacgatacca ccatcagagc aaattggaat ataaatacct gttttttcaa
    25861 agtattcgtc tctagcttga gcaacttcaa ttatagaagt agcttgtcca cgtcctatac
    25921 ctttttgttc acgagttata catatagaac caccaccaat accaacttta acgaagtcag
    25981 caccagcttc agctaggtaa aggaatcctt cgcggtcaac tacatttcca gctcctacct
    26041 taactgtatc accatatttt tcacgaatga aatcaagtgt tattttttgc cactcagaaa
    26101 aaccttctga agaatctata cataaaacat cagcaccagc ttcaactaaa gcaggaacac
    26161 gttcagcaaa gtctctagta ttaataccag ctccaactac atatctctta gaagaatcta
    26221 aaagttcaag tggattttct ttatgagaag agtaatcttt acggaatacc atatacataa
    26281 gattaccatt atcatcaagt acaggaagag aattaagttt gtggtcccat ataatattgt
    26341 ttgcttcctt taaagtaaca tctttgttag cataaactat agatgacata ggagtcataa
    26401 attcactaac tttagtatct aaatccatac gacttatacg ataatcacga cttgcaacaa
    26461 tacccaataa tttaccagtt gaagttccat cttcagtaac agctactgta gaatgtccag
    26521 ttttttcttt taagtcaagt atgtctttta atgtgttatc aggtttaata tttgaatcac
    26581 ttacaacaaa accagctttg tgggatttaa cacgtgctac catagcagct tcattttcaa
    26641 tagtttgaga accgtatata aatgaaatac caccttcttt agcaagtgca atagccattt
    26701 tgtcatcaga aacagattgc ataatagcag aagtcaaagg tatattcata taaatatctg
    26761 cttcctcgcc ctttttaaat ttagtaacag gtgtttttaa acttacatta gcaggtatac
    26821 aatcagtaga agagaaacct ggtacaagaa ggtattcact gaaagtatgt gatggtgtat
    26881 caaaataaaa cgccattttt taatcactcc ttatattttt atacttttta taattgatag
    26941 tattaattat aacttattct ttaattttat taaagtattt tgtcaaatat tgagattttt
    27001 gaaaaattta aactatttta attaaatttg tttaatatat attgccttat agggtaaagt
    27061 tgtatattaa ataatgttta gtgtttaata gtagctaatt taaaattaat ctttagtgat
    27121 tcatataatt acatttgtat agtttacatt gataatatta ctagagagtg tataaacctc
    27181 aaaaatacag ataaattaaa tctatattag tatatccaaa agtacattta ataaatttat
    27241 ttaactgaat tattattttc atttctatta aatttaaata ttttataaaa atagtattat
    27301 ttacaaaatc atatactcca aaatactaca taaataaaat taaacaaaaa ctaaaatgac
    27361 aaatgtcatt atctaaagtg acatagaaac ggtttcttaa agttgttact ataaaatata
    27421 aactctataa taaaagtata acaaaaggga atcatatcga tatagaatat aattcaagag
    27481 gaggtaaaat tatgagtagt tctaatcaat cgacaataaa gacatctata cagcgagtag
    27541 ggaaattttt aagtggtatg gtaatgccaa atataggagc ttttatagca tggggtctta
    27601 taacagcatt atttatacca acaggatgga tgcctaatga aaaattgagt actataggtg
    27661 acccaatgat aaaatacctt ttaccactat tgatagctta tactggtggt aaagcaatag
    27721 caggacaaag aggtggcgta ataggtgcag cagcagctat gggagtaatt gttggtgcag
    27781 atataccaat gtttatcgga gcaatgataa tgggaccatt tgcaggatgg gtaattaaga
    27841 agtttgataa atttgtagat ggaaagatac caacaggatt tgaaatgtta gtaaataact
    27901 tttcaatagg tataataggt atgcttcttg caatactagg attttatgca attggaccag
    27961 caatagtagc aggaacagct cttatagaat ctggagttca atttatagtt agcaaaagtt
    28021 tattgcctct agtttcagta tttatagaac ctggaaaagt attattctta aacaatgcaa
    28081 taaatcatgg tatattaggg cctataggta tagctgaggc aaaagaagca ggtaaatcta
    28141 taatgttctt actagaaagt aaccctggac caggtttagg agttctatta gcatattgga
    28201 tgtttagtag aggaagtgta aaacaatcag caccaggagc tgtaataata catttctttg
    28261 gtggtatcca tgaaatttac ttcccatata ttttaatgaa tccagtttta atattagcta
    28321 caatggcagg tggagcagca ggaatattga ctttttcaat attaggagct gggcttgtag
    28381 cagcaccatc accaggaagt atatttgcac ttatggcact agctccaaag ggaggattac
    28441 ttccagtatt agcaggagtt gcagtggcaa cagttgtatc attcttagtg gcagcaccat
    28501 ttgttaagag agcttcagca aatcaaagtg aagaagacag tacttcttta gaagaagcta
    28561 aagcaaaaat gagtgatatg aaatctgcaa gtaagaactc tgagaaaaat attgaggaaa
    28621 aacagcttga agtaaatgag attaagaaga ttgtatttgc atgtgatgca ggaatgggtt
    28681 cgagtgcaat gggagcttcc agatttaaaa atagaataaa aaacttagac ttaaatatag
    28741 aaattacaaa ttcatctgtt gataatcttc cagatgatac acaaatagta gtaactcaca
    28801 atacattagt tgaaagagtt gctaagaata attcaagtgt tgagatagtt tcaataaaca
    28861 attttttaaa tgacccaaat ttagatacat tgtttaaaag attagaatct aaataaaaat
    28921 aaaaagtacc actaattagg agtggataat ttgaaaaaaa agaagattac atctaggcaa
    28981 aaaaagataa tactaatgat agtagaaaat tcaaagaaaa acattccaat aactatttct
    29041 gagattgcag ggactttaga gttaagttca agaactgtac ttagagatat gtctggaatt
    29101 gaaaaatggt ttgatgaaaa tgattttaac tttgtaaaaa aaccaggagt gggattgatt
    29161 ttagaggaaa atatagaaaa tcaaaacttt ataattgagt tgttagaaga agaaaaaata
    29221 gaaaaagaat acagcaagga agaaagaaat ttaattatat tatcaaagct actagtttct
    29281 aacgagccag taaaatccta ttattttgca aaaatattaa aggtatctga aggggtactg
    29341 aataatgatt ttgccttagc aagtaaatgg ttggaaagat ttgatattga gcttgttaga
    29401 aaaccagggc ttggtgtcta tttaaaaggt caagaaaaaa gctttagaga ggcatatgta
    29461 aatcttatat acgattcatt caatgaaaaa gaaatactag atatggtcag aaatataagt
    29521 gaaaatatac aaacagataa agcaatagaa attttaagtg aaaatagact tttaaattta
    29581 atggacaggt gcataataag aaaggttgaa gctacattaa caaagaagct ttcagattta
    29641 gatgtaaatt tagctgatag tgcatatata gggcttgtag ttcatatttc attagcctta
    29701 cagaggataa aaaatggtga aaatataact atggataaag agtttctaaa agaattatct
    29761 ataacagaag agtttaaatt agctaaagaa atcgtaaaag gtatggaaac agattttaac
    29821 atggatatac cagtagatga ggttggatat ataactatgc atataagagg tgcaaagcaa
    29881 aggtcatctt caaaccataa ggctttaaac ttagatgata tagaaataat ggaaattaca
    29941 aataagatga tagatttggc ggaagatgaa tttaaaataa gtttaaaaaa tgacgagagg
    30001 ttatttaaag acctagcaaa tcacttaggg ccttctataa atagattgaa tatgggatta
    30061 gaaataagaa atcctctttt agatgaaata aaaagcaagt attcatatgc ctatgatgga
    30121 gttgaaaaga tttctagaat tataaaagat aaactaaata taaattcaat acctgaatca
    30181 gaaataggat atattgcaat gcactttgca tcagccatag aaaaaaatct tatgatgaat
    30241 actaatataa atattgttgt ggcatgtccc actggaatag gtacttcaag attcttatca
    30301 actaagattg aaaataaatt tcctaattta aatatacttg aaaccatatc agctataaac
    30361 atagatgaag agtatttaaa ggaaaaagat gttgatttaa tagtgtctac agttgagtta
    30421 aatacaagtt taaattatat atgtgtaggt ccatttatga gtttagatga tgaacagata
    30481 atcaaggaaa aaataaaatc tatagctcaa aataaattaa tcaatttaaa tgcgaaaaat
    30541 gatactaaga gtaagaataa agtatatgaa cagataacag aaagtatgaa tataggaaaa
    30601 gatattcttc aatttttaga ggaaattaga tttgaaaaat ttgaaagtaa agatttaagt
    30661 gagcttattg aagactcatc aaaaatattt gcaaaatcta gtgaagatat aatctctata
    30721 aaagaatcgt taaaagaaag attaaaaatt tcaattccat atatagaaga atcaaaaatt
    30781 ctattgttac attgtatgag tgaaagaata gatattatga agttatcaat aataaaacta
    30841 gagaatagaa tcgtgctaga ttcaaatgaa gaaatcgaca atgtagtttt tatgttactt
    30901 cctaaaaatt caccaagtta tcaaagacaa ataatgagtg aaataagtgg ttcattaata
    30961 gacaatataa tttttactaa taagataaat aaattctcta tagaagagat ggcattagag
    31021 gtaaaagata tagtttttaa tttttataca aacagattaa aagcatttat agataaataa
    31081 aattaatatt aaaaaggaag gttgatacta tgagtaaagt attgaatgag aataatatat
    31141 ttttaggatt agatagtgtt tcaaaggaag aagctataac tttagcagga agaaaattag
    31201 ttgaaaatgg atatgtaaaa gaagaatata taccagcaat gttagaaaga gaaaaagtaa
    31261 tgactacata tatgggaatg ggagttgcta ttccacatgg tgtaaatgaa gcaaaaaaag
    31321 aaatattatc ttctggaata gttatattac aattccctaa tggtatagat tttgatggag
    31381 aaaaagcata tttattaata ggaatagctg gtgttggaga tgaacattta gaaatactat
    31441 ctaatatagc agtagtttta gatgatgact taacagaaag actaaaaaat tcaaatgaca
    31501 aacaagcttt tatggaagct tttgccaact aatcttagga gggatttaaa atgaaaaagg
    31561 caattcagtt tggagcagga aatattggaa gaggttttat tggaggcttg ttagttaagt
    31621 cgggttatca tgtagtattt gctgatgtaa atgaggggat attaaactca ataaacaaag
    31681 ataaaaagta tactatacac ataagagatg tagaatgtat agatgaagta attgataata
    31741 taagtgcggt ttcatctata aaagaagaaa taatagatga gattgtacag gctgaaataa
    31801 taactacagc agttggacct ttagttttga ctaaaatagc ctctacaatt gcaaaaggta
    31861 taaaggctag aaaagaaaag ggattaacaa gtaatctaaa tataatagca tgtgaaaatg
    31921 caatatacgc aagttcttct ttaaaagaag aggtacttaa gtatttaaat aaggaagaag
    31981 tagaatattt agagatgtat gttggttttc caaactgttc agttgataga atagttcctc
    32041 ctggtaaaaa cgaaaatcct cttgatgtaa cagttgaaaa tttttatgag tggaatgttg
    32101 aaaaacaagg atttaaaggg gaaataccaa ctatagttgg aatgaattta gctgataatc
    32161 ttatggctta tatagaacgt aaattgttta cactgaatac aggtcatgct ataacagctt
    32221 atataggata tttaaaagga tataagacta tagaagagag tataaaagat aaatttatct
    32281 gtgatatagt caaaagtgca atggtagaaa gtggagaagg attgattaaa aaatacaatt
    32341 ttgactcaga agtgcactat aaatatattg ataaaatttt aaatagattt aaaaatccat
    32401 atcttaatga tgatgtatta agagtcggaa gggaaccatt aagaaaacta agtgataaag
    32461 atagattgat taaaccttta atgacagcaa aatcatatgg attatcagtt gataatttga
    32521 ttcttggaat aggtgcagcg cttcattata ataatagtga agatactcaa agtgtagaat
    32581 tacaagagtt gataaagtct attggggtaa aaaaagccgt tgctaaaata gctaatataa
    32641 gcaatgacga agaactttta aataatatcg aaaaaagtta tatatttatg aaaaatttat
    32701 aaatttactt gaaaaaaagt agttctgata aagtaacta
//
LOCUS       DAELLV010000016        32091 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.016, whole genome shotgun sequence.
ACCESSION   DAELLV010000016 DAELLV010000000
VERSION     DAELLV010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32091
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.016"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            104..487
                     /locus_tag="KPD58_000675"
     CDS             104..487
                     /locus_tag="KPD58_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264462.1"
                     /translation="MSTDKVVGIIDEETAELAGIEYTGKIYASSGVIKHIKKKHRCQL
                     SKDIFNDIIDTIKMVLKSPEYIGSHPKKPGKSVEFIKKIDDYILVATELDTKKGYLYV
                     SSMYAIKEAKLESRVNSGRVKQYDG"
     gene            993..1376
                     /locus_tag="KPD58_000676"
     CDS             993..1376
                     /locus_tag="KPD58_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBG0264463.1"
                     /translation="MNLTKKIEDKRKAKQRAEKVKKAKIATAGVVLGAVTGAVSGVLL
                     APKSGKETREDIKDASQQIAEKINMKTVDVKGKVSEKLEDKKGNFIESKKKIKKYIDN
                     KKSVENSSEEDKIIDIVEPVSVTEE"
     gene            1378..1728
                     /locus_tag="KPD58_000677"
     CDS             1378..1728
                     /locus_tag="KPD58_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264464.1"
                     /translation="MDNTITINISIIYVLLAILACIALVYLIIVLVKLHKILNNVGTM
                     LNENTQNINTTLNNLPNVVETFGEVGENMKDVTEVVTEVTADFLVTKDSVKSNMDVIV
                     DILTIIKNIFLDKK"
     gene            complement(1829..2287)
                     /locus_tag="KPD58_000678"
     CDS             complement(1829..2287)
                     /locus_tag="KPD58_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264465.1"
                     /translation="MIRTKVVELIATVCRENKPHKWVDENYTPYDKSGKVELMSIEDL
                     NELISSSGRADFLYSSRLQKLLNEVYINQSRASYISGCGLFWSSYWDILEEKFEEWLY
                     NSYIFFDEDDEYLEGMEDFELECKDVLMDVIETTSIDIYVQMIKRNITNY"
     gene            complement(2332..3120)
                     /locus_tag="KPD58_000679"
     CDS             complement(2332..3120)
                     /locus_tag="KPD58_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG0264466.1"
                     /translation="MKVVIIPGHTLIGKGTGAVGYINESKETRILNDLIVKWLKIGGA
                     TVYTGRVDESSNHLADQCAIANKQETDLAVQIHFNSNATTSTPVGTETIYKTNNGKTY
                     AERVNTRLATVFKDRGAKSDVRGLYWLNHTIAPAILIEVCFVDSKADTDYYVNNKDKV
                     AKLIAEGILNKSISNSQGGGENKVYENVIVYTGDADKVAAQILHWQLKDSLIIEASSY
                     KQGLGKKVYVVGGEANKLVKGDVVINGADRYETVKLALQEIDKL"
     gene            complement(3259..3696)
                     /locus_tag="KPD58_000680"
     CDS             complement(3259..3696)
                     /locus_tag="KPD58_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HBG0264467.1"
                     /translation="MNKKLENRCSWQEYFMRLCETVAERGTCDRAYVGAIIVNSENRI
                     VSTGYNGSISGDKHCSEVGHEMRDGHCIRTIHAEQNALYYCAKEGISVKNCSIYVTHF
                     PCLNCTKAIIQAGIKHIYYRTGYRIDEYAIKLLQSSNVLYTKL"
     gene            4518..5027
                     /locus_tag="KPD58_000681"
     CDS             4518..5027
                     /locus_tag="KPD58_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0264468.1"
                     /translation="MLSENIKTIRKSKGLSQQELAIKLNVVRQTISKWEQGLSVPDSD
                     MLISISEVLETPVSTLLGETIVESEVDSLKVISEKLEIINLQLAQRKNMRRKMFHWLF
                     ILLCAVILIGFVVLGGLNSPYLDWNYTDPETAVFGVAFHAFEWLFVRLVPIVLIGAII
                     GIFVTRKKI"
     gene            5432..5968
                     /locus_tag="KPD58_000682"
     CDS             5432..5968
                     /locus_tag="KPD58_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBG0264469.1"
                     /translation="MQGGVNIRQVKSFVFPVISLIFLDQISKVLIGLFLMDFEIDIIG
                     KFLRFNPVQNTNLSYGGNFIGILSNLWVLVLFNILVILVIISGYAFYKSKNEQTSYSV
                     KVIMSCGLAGTICSLIDKLFWGGSLDFLQIPSFFIFDLKDCYLTVAEIIFVVIGILHN
                     REISMKEYIYFCYRQFKK"
     gene            complement(6402..7202)
                     /locus_tag="KPD58_000683"
     CDS             complement(6402..7202)
                     /locus_tag="KPD58_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0264470.1"
                     /translation="MRLNKRSEGYINYVKGVKREKRNVLFYQLLILIGFIVIWELLAD
                     LNVINTFLFSKPSDIYNLFIQYAFSGQLFKHIGISVYETVLGLVIGTVLGILVAIALW
                     WSEKLSKILDPFLVVLNALPKTALAPIIIVWVGAGIEGIVVTAVTISVVVTILSAYNY
                     FMNIDEEKIKMLKSFGATKSQILFKLILPANTGNLINLTKINIGMAWVGVIVGEFLVS
                     RYGIGYLIVYGSQVFKLDLVMMGVFVLAICAWAMYAVVNIIEKIYNSR"
     gene            complement(7397..8995)
                     /locus_tag="KPD58_000684"
     CDS             complement(7397..8995)
                     /locus_tag="KPD58_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG0264471.1"
                     /translation="MKAAIYSRKSRFTGKGDSIENQIQLCIDYAKSIGVKDFLIYEDE
                     GFSGSNIDRPQFKKMMQDAKDKKFSYLVCYRLDRISRNVSDFSTLIEKLNELNISFIS
                     IKEQFDTSTPMGRAMMFISSVFAQLERETIAERIKDNMYELARSGRWLGGNTPHGFNS
                     EKITFLDENLKERSMYKLQVNDEQMEIVKLIYNKYLELRSLSKLYKYVYNNGIRGPRG
                     GKFDPSSLSRILRNPAYVKANDEILDYLKNSGMDVVGTPDRKCGILTYAKNTSNSIAA
                     IAKHHGVIEPEIWLEVQNQLDKNRDKTPRISTGKTALLSGLIKCGKCGSNMRITYKGK
                     KTDKDLKYYYICGTKKTLGSDSCNCKNLNGPLVESLVIDKIKNCKEESIISAFNSNNI
                     KTDNVFNIFKSETNLLKNQILDKERLIKNLVIELAKNTGSTASDYIVSQIESLNNEIS
                     ILKDKINILESSNINISSDSINIDIIVSNLKRFNDEVDNASLDDKRLLLSTIINSIVW
                     DSSLGNLNIVYMGAEVDNSVSLSN"
     gene            9323..9499
                     /locus_tag="KPD58_000685"
     CDS             9323..9499
                     /locus_tag="KPD58_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264472.1"
                     /translation="MEINVIEIFPKDKAKLNKIEMDKASWFVNIISKKYPKEALNEAF
                     STLEKELNISKANT"
     gene            9673..10803
                     /locus_tag="KPD58_000686"
     CDS             9673..10803
                     /locus_tag="KPD58_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBG0264473.1"
                     /translation="MNNFEIKTRIKFGEGSLKALQELKNKNVLIITDPFMVKSKTIDK
                     ITSNLINSTYQVFEEIVPDPPIEVVVAGIEAFKSINPDVIIALGGGSAIDAAKSIMDF
                     SKKILKINEVEFIAIPTTSGTGSEVTSFAVITDSQKKCKYPLVSDDLLPDMAILDPEL
                     VKTVPNFITADTGMDVLTHAIEAYVSTNSNDFSDALAEKAIKLVFKYLIKSYKDGNDI
                     EAREKMHNASCLAGMAFNQASLGINHSIAHVLGGKFHIPHGRTNSILLPHVIEYNSSI
                     VGYTNTTYSDTAKKYAQIAELVGLGSANIRISVRNLVNEIKKLQKEMNMPTKLTQCKL
                     SLEDITNEEREIAKLAIKDTCTLTNPKVPTEDDIINILKNIK"
     gene            11044..11394
                     /locus_tag="KPD58_000687"
     CDS             11044..11394
                     /locus_tag="KPD58_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBG0264474.1"
                     /translation="MTEESKQRVIQEYVPGKQVTLAHIIANPNEDIYKKLGLVLDKKD
                     AIGILTITPSEASIIAADVATKASNVSLGFIDRFSGSVVISGDVSSVESALNDVLEVL
                     GNMLNFSSTKITRT"
     gene            11402..11851
                     /locus_tag="KPD58_000688"
     CDS             11402..11851
                     /locus_tag="KPD58_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HBG0264475.1"
                     /translation="MKNIIFMGKTGSGKTTLCQKLDQLELKYKKTQSVELYNNAIDTP
                     GEYMENRNLYNALITTSADAKIIAIVYDATQEENYIAPGFASMFCKDVIGIITKINKA
                     TENEVNIAYERLSLAGASQIFKIDTVDNIGLEKLFKYLEKYKNIDEV"
     gene            11953..12528
                     /locus_tag="KPD58_000689"
     CDS             11953..12528
                     /locus_tag="KPD58_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBG0264476.1"
                     /translation="MGKKILLVDDEPLLRMDTKDILESHGYEIIGEASDGFEAVEMCK
                     KHKPDLVIMDIEMPILDGIKAGKIICKENLAGGVLLLTSFEDKEYVEKAKSIGAYGYL
                     VKTASEKSLIPTIEMCLNKVNEFEKMKKDLEKVNSKLNERKLVERAKGILIREFKISE
                     DEAYTRIRKLSMDKRTTMMEIAKMIIIGYDD"
     gene            12521..13930
                     /locus_tag="KPD58_000690"
     CDS             12521..13930
                     /locus_tag="KPD58_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HBG0264477.1"
                     /translation="MTNTIKKISTAQTYLNNNEIEKIIEVSKSLDLMASFYEADVFID
                     VLSKFDDEAIVVAHGRPKEKSMYCENVIGKKALIENEPGVIKCLKTGERVRDIKALTQ
                     EYKLVKQNIQPITLDDKVLAVLIVEKDISSEVKNEFNASEDEENKNLHQFIKLIGNND
                     FLVDNLNSAILIFDKNGKLKIKNRIATEIYKNLGFGYDIQDMHYNDLDIETNTFENIL
                     MDENYDETNEVSIGNMFFKIKTIYIKDDEFKVCKIVQDISDLKRKEEELVLKTVAIRE
                     AHHRVKNNLHTVISIIKKQSRLSQNDEVKQCLDNIINRVFAILSTHYLLSKEVDNNIS
                     IREGINLLVSNIQGGYLFSKDIDIRITGEDFKISGEKATAILLVINEILQNCYDHAFS
                     DREHGTIQILINKDNDYRNIAIIDDGIGFDSNNINKNSLGTYIIDSYITQMLKGNIER
                     KSSENGTEVLITIPTQINI"
     gene            14114..15547
                     /gene="eutA"
                     /locus_tag="KPD58_000691"
     CDS             14114..15547
                     /gene="eutA"
                     /locus_tag="KPD58_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HBG0264478.1"
                     /translation="MREELLSVGIDIGTSTTQLIFSKLIVENTESNFSVPRISIVDKE
                     IIYKSKVYFTPLISNNEINGQQIKDIVENEYKKANISKEDIHTGAVIITGETARKENA
                     NDVLHTLSGFAGDFVVATAGPDLESIISGKGAGAHIYSKEHSTTIVNIDIGGGTSNIA
                     SFKRGEVTDTGCLDIGGRLVKIDKISKKITYISPKIDKIIKDNNLNLQVGTMATVENT
                     KPIVDIMVDLLMESIGLKKQTPLFDFIITNKSISIDETIKNVSFSGGVADYIYYDGEI
                     KDYFEYGDLGILLGQAIKNCEYLKKVNVVKSMETIRATVVGAGSHTTEVSGSTITYTS
                     EKFPLKNLPVLKLSIDEETGTSKQISEAIKNKLNWFKLENDLQKVAIAMIGKKNPTFL
                     DIQEYAKGFVDGMQQIIENDLPFIVIVENDMAKVLGQAIYALLNYEKEIVCIDSVKVE
                     NGDYIDIGKPIVEGKVLPVVIKTLVFN"
     gene            15565..16929
                     /locus_tag="KPD58_000692"
     CDS             15565..16929
                     /locus_tag="KPD58_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HBG0264479.1"
                     /translation="MILNTKLFGRTYQFKGLYDVMAKANEEKSGDKLAGLAAESAEER
                     IAAKVVLSHIKLEDIRNNPAVAYEEDEVTRIIQDAVNEKVYEEIKNWTVSELREWLLD
                     SNTTSLDIRRISRGLTSEMIAAVAKLMSNMDLVYAAKKIKVTAHCNTTIGEQGTLSAR
                     LQPNHPTDDPDGILASLLEGLTFGIGDAVLGLNPVDDSVESVTKVLKRFEEVKQKYQI
                     PTQTCVLAHVTTQMEAIRNGAPADLIFQSIAGSEKGNEAFGFNGDTIEEARQLALKHG
                     TATGPNVMYFETGQGSELSSEAHHGIDQVTMEARCYGFAKKYEPFLVNTVVGFIGPEY
                     LYDAREVVRAGLEDHFMGKLHGLPMGVDVCYTNHMKADQNDMENLAILLTTAGCTYFM
                     GIPHGDDVMLNYQTTGYHETASLREMFGLTAIKEFDAWMEKMGFSKDGKLTELAGDAS
                     VLLG"
     gene            16942..17823
                     /gene="eutC"
                     /locus_tag="KPD58_000693"
     CDS             16942..17823
                     /gene="eutC"
                     /locus_tag="KPD58_000693"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014952556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HBG0264480.1"
                     /translation="MNEKDLKALVEQLVGQMVGELDTNVVSETVKKATEVVVDNNAYI
                     DDITEVDIRKQLLVKNPKDAEAYLDMKAKTPARLGIGRAGTRYKTETVLRFRADHAAA
                     QDAVFSYVDEEFIKGNNMFAVETLCKDKDEYLTRPDLGRKFSPETINNIKSKFGTNQK
                     VLILVGDGLSSAAIEANLKDCVPAIKQGLKMYGIDSSEILFVKHCRVGAMDHLGEELG
                     CEVICMLVGERPGLVTAESMSAYIAYKPYIGMAEAKRTVISNIHKGGTTAVEAGAHIA
                     ELIKTMLDKKASGIDLK"
     gene            17843..18496
                     /gene="eutL"
                     /locus_tag="KPD58_000694"
     CDS             17843..18496
                     /gene="eutL"
                     /locus_tag="KPD58_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HBG0264481.1"
                     /translation="MKNDVIRPNILGIKIISNISPEMAQKLELKSHHKSLGLITADCD
                     DVTYTALDEATKASEVDVVYAKSMYAGAANASTKLAGEVLGIIAGPSPAEVRSGLNAV
                     VDFLEYGATFISANDDDSIAYYAHCVSRTGTYLSEVAGIREGEALAYLVAPPLEAMYA
                     LDAAMKAADVKMCELFAPPTETNFGGALLTGSQSACKAACDAFAEAVKSVADNPTGF"
     gene            18507..19208
                     /locus_tag="KPD58_000695"
     CDS             18507..19208
                     /locus_tag="KPD58_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBG0264482.1"
                     /translation="MDAIGLIEVIGYVAAIEASDACLKSANVNIVRVDKVGAGIVTLT
                     ICGDVGAVKAALEAGEIAASRVGTLRTSHIIPRIHNEVTNALFKTKEPKVCEVSEDKE
                     KNFEITLEITDNVSKNLSEKENVILDVNENSGNTENLYSDIDKTNKKIETVDDSLTEA
                     NEEKIENTNDKEVLEQVKIENSDFDRDDLSKNSVKKLKAMAKKLDSSITYKDLNVLKK
                     EELINLVKKLSRRDR"
     gene            19211..20680
                     /locus_tag="KPD58_000696"
     CDS             19211..20680
                     /locus_tag="KPD58_000696"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HBG0264483.1"
                     /translation="MTLLDKDLVSIQEVRTLVRRSKVAQEKLSKMSQEQIDKIVKSVV
                     DAAFNNATKLAKMANEETGFGKWEDKVIKNTFASMGVYETLKNTKTIGIIGEDKEANT
                     IDIATPVGVVAGLIPSTNPTSTVIYKTIISLKAGNTIVFSPHPNAKNCILETVNIISE
                     AAIKAGAPEDSILCITNPSIVGTNELMKHKDTNLILATGGEAMVRAAYSSGTPAIGVG
                     PGNGPAFIDKSANVKLAIKRIIDSKTFDNGVICASEQSIVIEKSMEETVLKELKNQGA
                     YVLNEEESERLAKFILRANGTMNPQIVGKSVEVIANLADLTNVPKNTKVLVARENKVG
                     HNVAYSREKLTPILAFYVEENVEAIMNRCREILLNEGAGHTFSMHVNDEELVKRFALN
                     MPVSRILVNTPSALGGIGASTNLVPALTLGCGAIGGSSTSNNIGPLDLINVKRVAYGI
                     KEIEELGRDKSQVEKSMFDSMDKEELINLLVQKLVKELA"
     gene            20776..21063
                     /gene="eutM"
                     /locus_tag="KPD58_000697"
     CDS             20776..21063
                     /gene="eutM"
                     /locus_tag="KPD58_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HBG0264484.1"
                     /translation="MASANALGMIETKGLVGAIEAADAMVKAANVQLVGKEQVGGGLV
                     TVMVRGDVGAVKAATDAGAAAAERVGELISVHVIPRPHFEVDAILPKVSAE"
     gene            21190..21951
                     /locus_tag="KPD58_000698"
     CDS             21190..21951
                     /locus_tag="KPD58_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP--cob(I)alamin adenosyltransferase"
                     /protein_id="HBG0264485.1"
                     /translation="MGVLTESEVKRKIGKNKNNITEFYIEKGQIITPSAKSYLAERSI
                     ELKYVDKLSDVNNEPKEVVKEVVKEKKYRYTTVFGAKLDEKPEHMTQLNGNLLVFKDH
                     KRIILRGKIDSLESEILKVQILCQKLDMPKLVKELQEVLDFVRNIMRCEVLDEKLGDF
                     KLLGLTPQELREQSHYPKKYFGIGHEFPSYEMGEVVVAINSIRSNTREVELAAYEAFK
                     GEYGNVHREDLIKALNRLSSVFWIMIYKVRTNKYK"
     gene            21964..22593
                     /locus_tag="KPD58_000699"
     CDS             21964..22593
                     /locus_tag="KPD58_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HBG0264486.1"
                     /translation="MENLLNEIVEEVITRVKKEAFIEVEASGRHVHLSREDVDKLFGE
                     GYTLTKLKDLSQPGQYACKERVTITGPKGSIKNVVVLGPCRNETQVEISLTDGSTLGL
                     KAPIKQSGDLEGTLSIKISTQYGEVELDKGLMVAKRHIHMTPKDAEKFNVCDKEIVEA
                     KVMGQRPLTFDDVVIRVSDSFKTYMHIDYDEANACGYSKGTVAKIIKKA"
     gene            22635..23360
                     /locus_tag="KPD58_000700"
     CDS             22635..23360
                     /locus_tag="KPD58_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02536 family ethanolamine utilization
                     protein"
                     /protein_id="HBG0264487.1"
                     /translation="MNYDNLVDLITEEIYKKINSNEIKISNKPKAVIVFEQDNNKFNL
                     LKDEFEIVEFDKSIRECEIVIVSRLCMRGLSNIALGNSTSDEERFILKMIMKGKEVYV
                     LDEGIEYKKYKDTAPKALYKKFMSFEDEICKFGVEIIKDLNVITKNKLNIKKELESTR
                     GSKKEDTKVLDKDVLNLISVESVKIDNEFSLDLRNKKLISEADLRKPTINGVKNILVN
                     KKSIITPLAVDFMRIHHLKLKKV"
     gene            23373..23645
                     /locus_tag="KPD58_000701"
     CDS             23373..23645
                     /locus_tag="KPD58_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HBG0264488.1"
                     /translation="MDIGKVVGNVWATRKDEKLCGQKLLVVKILKTKDTYKEGFMVAA
                     DNAGAGNGDLVLISKGSSARESIEKSHVPVDATIVGVVDSFEVFDE"
     gene            23638..24189
                     /locus_tag="KPD58_000702"
     CDS             23638..24189
                     /locus_tag="KPD58_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBG0264489.1"
                     /translation="MNKSIGAIEFKSIAKGIEVSNEMIKKSSVDVLYLKSICPGKFLI
                     IVGGETSYINECVDYGIKLGEGYIVDNFVINAISQEILDGFKNKYQKLDSIVSIGVVE
                     SSKVCAGIKMLDKTLKSGDLVLVKLQLSFAIGGKLVYIVAGDLSSLEYALKESENVVR
                     EKEVIYKTVIPSVDSQIIKSLIK"
     gene            24201..25292
                     /gene="eutH"
                     /locus_tag="KPD58_000703"
     CDS             24201..25292
                     /gene="eutH"
                     /locus_tag="KPD58_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HBG0264490.1"
                     /translation="MSINEIIIYVMVVFMVLGAIDKCIGNKFGLGEQFEEGIMAMGSL
                     AVAMVGVICLAPVLADVLRPIVVPVFNILGADPAMFAGSLLANDMGGAPLALELAKDP
                     NAGLFGGLIVGAMMGPTIVFIIPVALGIIEKEDQKFLATGILAGIITIPIGAFVGGLV
                     AGFEVMMVLRNLVPIIIFAVVIALGLLKFENAMIKGFTYFGKGVVIVITLGLAAAIVE
                     ALTGIVVIPGMKPISEGIEIVGDIAIVLAGAFPLVFVITKVFNKPLMALGKVLGMNDV
                     SAAGLVASLANCIPMFGMMKDMDNRGKIINVAFSVSAAFVFGDHLGFTAGFNSNMITP
                     MIVAKLVGGITAVLLAMVIANKTLKKENV"
     gene            25294..25767
                     /locus_tag="KPD58_000704"
     CDS             25294..25767
                     /locus_tag="KPD58_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF861 domain-containing protein"
                     /protein_id="HBG0264491.1"
                     /translation="MDISNIDKNLIETLVRQIIEEKISGTKDTVDFVRNKDISGITSI
                     KLPTVKVSESDRLDTGNPSDVVYTKDLFTLEESPRLGCGMMEMKETTFDWTLNYDEID
                     YVIDGTLDIIIDGRKVSASSGELIFIPKGSKIQFSVPDYARFIYVTYPADWASQN"
     gene            complement(25887..25988)
                     /locus_tag="KPD58_000705"
                     /pseudo
     CDS             complement(25887..25988)
                     /locus_tag="KPD58_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            26283..26804
                     /locus_tag="KPD58_000706"
     CDS             26283..26804
                     /locus_tag="KPD58_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0264492.1"
                     /translation="MEEIVKKEQASMNDIFLYLKAKSNSLYKFVILYNEFINKPKDYG
                     TGELINMVEVHMLTAIEENPGISPTQLAKIWNRTKGAISQTISKLEKKGYITKEKKEG
                     NAKTILLYPTTSGVELSKAHKLYDTIDVTRTFEYLIKECTLEEIDSFYKVIDLFINLL
                     EESPQENNDSEKV"
     gene            27183..28358
                     /locus_tag="KPD58_000707"
     CDS             27183..28358
                     /locus_tag="KPD58_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0264493.1"
                     /translation="MNLKQLSEKYEEYVIQMRREFHKYPELSFKEIRTTQRIREELQK
                     IGVPFVSIEPNIVIATIQGKNEGKTIAIRGDIDALPMQEDSDVEYKSEYDGIMHSCGH
                     DAHGAMLLGLAHMLNDIRKDLKGRVLLVFQAAEEVGGGHKEIIQYFKENGGPDRLIAT
                     HVWSDIEAGKISVEPGPRMAGGSGWRIDITGRGGHGSRPDQSIDPIKPACDIVLKVSS
                     IPVNHISVLEQCVVHACAIHGGTTIGNVIPNNAEVIGGYRYFTEESGKKVFDIIKQMS
                     NGVGQVYGVDVKVTHTGGIGPVINDEEAVKMAKEIVSNIDGLELDSYEPICASDCYGE
                     ILKEYSGFYCYLGVGNKEKGIIYAQHHPKYNIDEDTLKLGCEFMAKYAVEFLNKNNN"
     gene            28393..29931
                     /locus_tag="KPD58_000708"
     CDS             28393..29931
                     /locus_tag="KPD58_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBG0264494.1"
                     /translation="MQGALKENKKKSGGGFLGWIEKVGNKMPHPLALFTYITLIVVAL
                     SAIASFLGFSATSPATGKLIKVMNLISAKGLVLFMQEFVKNFQNFPVLGVVLVMAVAT
                     GVCEKTGFFSTAIKMSLSKVKGNAVVFIIAFIGVFANQAGDAAFVLVPTLAGAIFYGL
                     NRNPLAGIFCGFASVGGGFATAVIPGGWDVTLTPITVSAAQTIQPAFDMTLLASYYAL
                     FVSAFIVATVSTIVTVKFIEPKLGKYTGKPEGIDDNQGFEVTKEQAKAAKLAGFTVLA
                     YVALLIALCIPANSFLRSPEGSLIFGAPLMSCLQFFIVILFFLPGIVYGMRVGKIKTV
                     KDLNDILCQSMAAMAPFIVLAIVIGQFLTLFSVSNLAQVLAIKGGALLNSLPLPPQAI
                     ILLFLVLVAFINIFVISGSTKWMIFGPVFVPMLMQLNIHPAFTQFVYRLGDSVTNHLS
                     PLNAFFIILLATVQKYDKNAGMGTIFSAMIPYTIGYFVVLAVLVVVWMTIGIPVGIGG
                     QVWL"
     gene            29963..31165
                     /locus_tag="KPD58_000709"
     CDS             29963..31165
                     /locus_tag="KPD58_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG0264495.1"
                     /translation="METTEIRYNFDEVIDRSGTNCLKYDLKSQFMPESPEDALPLWVA
                     DMDFACAPEIINAMHERIDRKIFGYSSQQTDDYYEAVCGWFKRRFDWKIEKENIFFSP
                     GVVPALGFFTQILTQKGDGIIIEKPVYYPFEDKITNNERKVVNSPLKFEDGHYSMDYD
                     GFEELAKDENNKVFILCSPHNPVGRVWKKEELRKIVDICKKYDLWIISDEIHCDIIRK
                     GEKHIPLEVACPDYKDRIITCTAPSKSFNLAGMQLSNIVINNKELQKKWMLETDSKLA
                     IFLPNPFAIVATKVAYNDCEDWMNQVNEYIDGNIKYVEDFVNEYMPKVKIIKSQGTYL
                     VWLDFRGYGLDAKQLEYVMLNKAKVLLDEGYIFGEEGAGFERINVASPRTIIEECMNR
                     IKCAFEGL"
     gene            complement(31332..31931)
                     /locus_tag="KPD58_000710"
     CDS             complement(31332..31931)
                     /locus_tag="KPD58_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0264496.1"
                     /translation="MNDNIVEVKNINMNFYTKEGELEVLKDVNFNLKEGEILTLLGPS
                     GSGKSTILNILTNLLKPTSGDIKITGNIGYMFQKDNLLEWRNIMDNITIGLEIQGKKD
                     KKSLDRVESLLKTYGLWDFRSMYPKELSGGMRQRVALIRTLSVNPDILLLDEPFSALD
                     YQTRLLVCDDVYSIIKNENKSTILVTHDIGEARCIKVQL"
BASE COUNT        12541 a         3971 c         5378 g        10201 t
ORIGIN      
        1 atcttttctt tttggaataa actatacttt aaatatagat atttaattta aataaatttt
       61 acagtattaa tagtaaatta tcttagaaag gaatgtgctt tatatgtcta cagacaaagt
      121 tgttggaatc atagatgaag aaacggcaga acttgcagga attgaatata ctggtaaaat
      181 ttatgcttct tctggtgtta taaaacatat aaagaaaaaa catagatgtc aactgagtaa
      241 ggatattttt aatgatataa tagatactat aaaaatggta ttaaagtcac ctgaatatat
      301 aggaagtcat cctaaaaaac ctggaaaaag tgtggagttt ataaaaaaaa ttgatgatta
      361 tattttagta gctacagaat tagatacaaa aaaaggatat ttatatgtat cttcaatgta
      421 tgctataaaa gaagcaaaat tagaaagtag agtaaacagt ggaagagtaa aacaatatga
      481 tggttaacat aatattatta attttcccta aaatattgaa aaatattatt atataagtta
      541 taatattaat atacacgtag atatttatta gttagatttg aggttggaaa aggcacccaa
      601 tacgctacta tagagtagtt aatagagatg caggatacgc cgccctgcca aatctaatta
      661 aattaaaaag agaggattaa ttcctctctt attgtttttt tgagatacta atttaatcta
      721 tatagaaaga ttgaaggtca tttatactaa aagttttcag ttatatacat taaactaatt
      781 aaataagata tttataatct atatgtgata catattatat attaataaaa gaaaaatata
      841 tagaaaatcc ttataaaaat ttaaattgta atgaatagga agtaattaaa aagaattttt
      901 attgattttt aaaaatttag ttagaaacta tatcattatg gtaaaaaatg tattataatg
      961 tttagaaata ttataataag gagcgattaa acatgaatct aacgaagaaa atagaagata
     1021 aaagaaaagc taaacagaga gctgaaaaag ttaagaaagc taaaattgct acagctggtg
     1081 tggtattagg agctgttact ggtgcagtga gtggagtact tttggctcct aaatctggaa
     1141 aagaaacaag agaggatata aaagatgctt cacaacaaat tgctgaaaag ataaatatga
     1201 aaactgtaga tgtaaaaggc aaagtatctg agaagctaga ggataaaaaa ggaaacttta
     1261 tagaatcaaa gaaaaaaata aaaaaatata tagataacaa aaagagtgtt gaaaattcta
     1321 gtgaggaaga taaaataata gatatagttg aaccagttag tgttactgag gaataatatg
     1381 gacaatacaa taacgattaa cataagtata atatatgtat tattagctat acttgcttgt
     1441 attgctttag tttatttaat tattgtactt gttaaactac ataagatttt aaataatgtt
     1501 ggaactatgt taaatgaaaa tactcaaaat ataaatacaa ctttaaataa tcttcctaat
     1561 gttgtagaaa cttttggaga agtaggagaa aatatgaaag atgtaacaga agtagttact
     1621 gaagttacag cagattttct tgttactaaa gacagtgtca aatcaaatat ggatgttata
     1681 gttgatatat tgactataat taaaaacatt tttctagata aaaaataata agttaatata
     1741 aaataagcct ataagatata atttaaacga ccttaaaata gcatttataa ggtcgttttt
     1801 tattagcttg atttgacact atgtgttttt aataattggt aatattcctc tttatcattt
     1861 gaacatatat atctatgctt gttgtttcaa ttacatccat aagaacatct ttacattcaa
     1921 gctcaaaatc ttccattcct tctaaatatt catcatcttc atcaaaaaag atataagaat
     1981 tatatagcca ttcttcaaat ttttcttcaa gtatatccca atacgaactc caaaataatc
     2041 cacaacctga tatataagat gctctacttt gatttatata aacctcattt agaagctttt
     2101 gtaatctact gctataaaga aaatctgctc ttccactgct tgatattaat tcatttaaat
     2161 cttctattga cattaattca actttaccac ttttatcata aggagtataa ttttcatcaa
     2221 cccatttatg tggtttgttt tctctgcata ctgttgctat caactctaca acttttgttc
     2281 ttatcatttt tttatcctaa cttatttatt ttttataaaa taatcatgat tttataattt
     2341 atctatttct tgtaatgcta atttaacagt ttcatatcta tcagcaccat ttattactac
     2401 atcacctttt acaagtttat tagcttctcc acctacaaca taaacctttt tacctaaacc
     2461 ttgcttataa ctagacgcct caattattaa agaatctttc aattgccaat gtaaaatctg
     2521 agcagccact ttatcagcat cacctgtata aacgattaca ttttcataca ctttattttc
     2581 ccctccccct tgagaatttg atatagattt gtttagtata ccttctgcta tcaatttagc
     2641 aactttatct ttattattaa cataataatc tgtatctgct ttactatcta caaaacatac
     2701 ttctattaat attgctggtg caatagtatg attaagccaa taaagacctc taacatcaga
     2761 ctttgcaccc ctatctttga atactgtagc taacctagta ttcactcttt ctgcataggt
     2821 tttgccatta ttagtcttat atattgtttc tgtccctact ggagttgaag ttgtagcatt
     2881 tgaattaaaa tgaatctgaa cagccaaatc agtttcctgt ttatttgcta tagcacattg
     2941 gtctgctaaa tgattacttg attcatcaac tcttcctgta taaacagtag ctccacctat
     3001 tttaagccat ttaacaatta agtcattcag tattcttgtt tctttgcttt cgtttatata
     3061 cccaacagct cctgtacctt ttccaattaa agtgtgccct ggtattatta ctactttcaa
     3121 actaacatct cctttacaat taaatttaac ttctatttaa tatatgcata acctagaatt
     3181 ttgtttcttt ttatatctta tacttttatt ataacctatt ttgtaaaaat atagaatatt
     3241 aagtaaataa tgttaaaatt ataattttgt atataagaca ttacttgatt gcaataattt
     3301 tatagcatat tcatctattc tatatccagt tctataatat atatgtttaa ttccagcttg
     3361 aattatagcc ttagtacaat ttaagcaagg aaagtgagtt acataaatag agcaattttt
     3421 aactgaaata ccttcttttg cacaataata aagggcattt tgttcagcgt gaatggttct
     3481 aatacaatgt ccatctctca tttcatgacc aacttcacta caatgcttat ctccagaaat
     3541 actaccatta tatccagtag acactattct gttttctgaa ttgacaatta tagctcctac
     3601 ataagctcta tcacaagttc ctctttcagc tactgtttcg cacagtctca taaaatattc
     3661 ttgccatgag catctatttt caagtttttt attcaaatta tatacctcca aaacttatat
     3721 ctaatatata agatttatac cttaaaaata atattaaaaa caaatttata cgataaataa
     3781 aatttattaa tactgttttt agtctaaatt atttcatttt aaattaaagt tacaaggaat
     3841 ctaaaattat atatatcttt cttcgtaata ttaatgttac aatataaaaa actgtctcaa
     3901 taaaatagag acagttttac agtcaaagtt tataatacaa gttcatattt ttctttaaat
     3961 tcttttacag cttcgggcca tgccttttgt tctgtaatac ctttttcttt agctatttta
     4021 attgcaatct ctcttatcat tgcagcgcct tctaatattc ccatttttaa tctcccttca
     4081 aatatctttt tgtatagaat ataaatagtg ctaagattta ttacatgctg catatacaat
     4141 atctatacaa cacataactt gtaaattcct tttattttca tacgtcttta accgacatat
     4201 aattatattc aataagtttt aaaattatga actcaattat aattattttt atttttttag
     4261 aatctaaaca ctaaaaattg taggtaaatt tgtcttatgc ctttataaat atttctacag
     4321 ttttaattac tggacatatc tatttttatt ataaaaagcg cttaaaaatc gagaatatcc
     4381 gcataaaaac tgtatataag caaaacggtt aaaggaaaaa gaagaaattt acttgaatgt
     4441 taaaatgcgt tgcttgaagc aatgggcagt tttgcctata atatcggtaa caactgaaag
     4501 aaaggtggtt agccctaatg ttaagcgaaa acattaaaac aatcaggaag tcaaaaggac
     4561 tttcacaaca agaacttgct ataaaactga atgtagtgcg acaaacaata tctaaatggg
     4621 aacaaggact gtcagttccc gattctgata tgttgatttc catatcagag gtgcttgaaa
     4681 cgcccgtaag cactttactt ggagaaacta ttgttgagtc ggaagttgat agcttaaaag
     4741 tgatttctga aaaattagag attataaact tgcaacttgc acaaagaaaa aacatgagaa
     4801 gaaaaatgtt tcattggtta tttattttat tatgtgcagt catattaata ggttttgtgg
     4861 tcttaggggg actaaatagt ccttacttgg attggaatta taccgaccct gaaaccgctg
     4921 tttttggagt agcttttcat gcttttgagt ggctatttgt cagattagta ccgattgtcc
     4981 ttataggagc aattattgga atctttgtga cacgtaagaa aatatgaagt aactctgctt
     5041 tttataatac agcaagcgtt ccttaaaagc gtcattctac atggcaatcg aggacgacta
     5101 tgtaaggaag aacctttttg atttccaact aagcgaagtt attgaggacg acagtaagac
     5161 caaaaccgca ttaagtaaag tacaggaagc aagactgctg gacttatact ctcatgcgtc
     5221 agatgcagga aatgcgaggt ctggtagcat gatttacaac gatttgtaca acgactgtga
     5281 gtagaaatat atggagataa gtattatcta cgaaatcaga aaagaaatga cttgataatt
     5341 tactcatgtt ataattattg atgtgaaatt aataaatcga aattcctttt ttggatttaa
     5401 caatactgag taaggaaaaa gtgaatgact tttgcaagga ggtgttaata tcaggcaagt
     5461 aaagtctttt gtttttccag ttatatcctt aatattctta gaccaaatta gcaaagttct
     5521 tataggatta ttcttaatgg actttgaaat tgatataatt gggaaatttt taagattcaa
     5581 tcctgttcaa aatacaaatc tctcttatgg gggaaacttt attggtattc tatctaattt
     5641 atgggtattg gtattgttta acattttagt tatattagtt attatatctg gatatgcttt
     5701 ttataaatca aaaaatgaac aaacaagcta ttcagtaaaa gtaattatgt cttgtggact
     5761 tgctggtaca atatgtagct tgatagataa attattttgg ggaggaagtt tagatttttt
     5821 gcagatacca agctttttta ttttcgactt aaaagactgc taccttactg ttgcagaaat
     5881 aatatttgtt gtcataggaa ttttgcataa tagagaaata tcaatgaaag aatacatata
     5941 tttttgctat cgtcagttta aaaaataaat ttagaagata aatttaagtt tcaaggtaaa
     6001 cttatttaat cacagcaaat agttggctta cttaagcttt tgggctaaac tgtgagaact
     6061 aattaagtct ctgctatcta acaaacagag cacaaaaata aaaattaaat attaaatata
     6121 aagcacagtt aattttgatt aaaaagttga ctgtcttttc ttttgtagaa atttaagcat
     6181 aggtaataaa gaaatgactg catttaagca ttatgtatat aattggaggc aataaaaagc
     6241 cagcaaagcc cataaaatag agctgtgcta gcaacatgat gagttataag aagataatca
     6301 aaaacttacg ctttatttta atttaaaaaa atgattactg taaagaaatc attttttatt
     6361 ataatcactg atacatcaaa tattctaaat aaaattttta tttatctgga attgtatatt
     6421 ttttctataa tattaactac agcgtacatt gcccaagcac agatagccaa tacaaatact
     6481 cccatcatta ctaaatctaa tttaaatact tgagaaccat atactatcaa gtagcctatt
     6541 ccatatcttg aaactaaaaa ttctccaact attacaccta cccatgccat accaatatta
     6601 attttagtta aatttattaa atttcctgtg tttgcaggta atatgagttt aaataatatt
     6661 tgagatttag tagctccaaa gctttttaac atcttaattt tttcttcatc tatattcata
     6721 aaataattat aagctgaaag tatagtaaca acaacagaaa tcgttacagc tgttacgacg
     6781 attccttcta ttccagcacc tacccaaact ataattatag gagctaaagc agtcttagga
     6841 agagcattta aaactaccaa aaatgggtct aatatttttg ataatttttc tgaccaccaa
     6901 agagcaattg ctaccaaaat acctaataca gtacctataa caagaccaag aacagtttca
     6961 tatacagata ttccaatatg tttaaataat tgaccactaa atgcatattg aataaataaa
     7021 ttgtaaatat cacttggttt gctaaataga aatgtattta taacatttaa gtcagctaaa
     7081 agttcccaga ttacaataaa accaattaaa attaatagct ggtagaataa tacatttctt
     7141 ttttctcttt ttacaccttt aacatagttt atatatcctt cagatctttt attaagcctc
     7201 atgtgtatct agctccttcc ataatatgtc gaaatacttt tgaaaatcag gaactttacg
     7261 tgttttgaga ggcgtttttg ttccatccat gttaagttca atatcgtaaa tattttttat
     7321 ggtagctggt cttttagata gtattgcaac cttatctgat atgcttattg cttctgctat
     7381 agctaaaaaa gtgcaactaa ttgcttaaag aaacagaatt atccacttca gcacccatat
     7441 aaacaatatt aagatttcct aaactgctat cccaaacgat actattaata atagtagata
     7501 ataacaatct cttatcatct aaactggcat tatcaacttc atcattaaat ctcttcaaat
     7561 tactaacaat aatatcaata ttaatactgt cactactaat attgatgtta ctagattcca
     7621 aaatattaat cttatccttt aaaatactaa tctcattatt taggctctca atctgggaaa
     7681 ctatataatc agaagcagta ctaccagtat tttttgcaag ctctataact aaatttttaa
     7741 taagtctttc cttatctaaa atttgatttt taagtaaatt agtctcagat ttaaaaatat
     7801 taaaaacatt atcagtctta atgttattac tattgaatgc acttataata gactcttctt
     7861 tacaattctt aattttatct ataactaaac tttcaactaa tggaccatta agatttttac
     7921 agttgcaact atcagaccca agtgtttttt tagtaccaca aatataataa tactttaaat
     7981 ccttgtcagt ttttttgcct ttataggtaa ttctcatatt agaaccacat ttaccacatt
     8041 ttataagacc agataaaaga gcagtttttc cagttgatat ccttggagtt ttatcacgat
     8101 ttttatctaa ttgattttgt acttcaagcc atatttcagg ttctatgact ccatggtgtt
     8161 ttgcaattgc tgctattgag ttagatgtat ttttagcata agtaaggatt ccacattttc
     8221 tatctggagt acctacaaca tccataccac tatttttaag gtaatctaaa atttcatcat
     8281 tagcttttac atatgctgga tttctaagta ttctagataa agaactaggg tcaaatttac
     8341 caccacgagg accccttatt ccattattat agacatattt atataactta gataaggacc
     8401 taagttctaa atatttattg tatattagct ttacaatctc catttgttca tcatttactt
     8461 gaagcttata catacttctt tcttttagat tttcatctaa gaatgtgatt ttttcactgt
     8521 tgaatccatg aggtgtattg cctccaagcc atctaccact acgggctaat tcatacatat
     8581 tgtcttttat acgttctgct attgtttctc tttctaattg agcaaataca cttgatataa
     8641 acatcatagc tcttcccatt ggagttgaag tatcaaattg ttctttgata gatataaatg
     8701 atatgtttag ttcatttaat ttttctatta aagtagagaa gtcagataca tttctcgata
     8761 tcctatctaa tctataacaa actaggtaac taaatttctt atctttggca tcctgcatca
     8821 ttttcttaaa ttgtggtcta tctatattgc taccactaaa tccctcatct tcatatatca
     8881 aaaaatcttt tacaccaata gactttgcat aatcaataca taattgtatt tgattttcta
     8941 tagaatcacc ttttccagta aatcttgatt ttcttgaata aattgcagct ttcaaaatta
     9001 aaacccccta taagttagga tatatatttg aaattattta tatacaacct tttaagtaaa
     9061 cttattagaa tgaatttatt aattaggtaa atttatataa ttctactttt tataatatat
     9121 gactatttat tttatttctt tactattcaa gttctaaatc aataataaat tttgatattt
     9181 aattgtatca ggacaaatct ctaatttcat agattattaa tataaaatca taggttagtg
     9241 tgaaaaagtt gaaatttaaa tattttaaaa gataattttt ttgaactaat aggtataaca
     9301 aaaatttata gggggattgc atatggaaat caatgttata gaaattttcc ctaaagataa
     9361 agctaaactt aataaaatag aaatggataa agctagttgg tttgtaaata taataagtaa
     9421 aaaatatcct aaagaagctt taaatgaagc atttagtact ttagaaaaag aattaaatat
     9481 aagtaaagct aatacataag ctttgattat tgattttata tactattggt tttatacgct
     9541 atgacttgac attatattaa tcaaatagta tatttttaaa tatagcacaa tgaagtgtta
     9601 aaagagcaat ggagctccat agataaagtc tatggagctt ttttgcgtat ttaacttaag
     9661 gaggatagga ctttgaataa ctttgagatt aaaactagaa taaaatttgg agaaggctca
     9721 ctaaaagctt tgcaagaatt aaaaaacaaa aatgtactaa taataacaga cccttttatg
     9781 gtgaaatcaa aaacaataga taaaataaca tctaatttaa taaactcaac atatcaagtt
     9841 tttgaagaaa tagtaccaga cccaccaata gaagtagtag tagcaggaat agaagcattt
     9901 aaatctataa atccagacgt aataatagca ttaggtggcg gttctgcaat agatgcagca
     9961 aaatctataa tggacttttc taaaaaaata ttaaaaatta acgaagtaga gtttatagca
    10021 ataccaacaa caagtggaac aggttcagaa gtaacatcat ttgctgtaat aacagatagt
    10081 caaaaaaaat gtaagtatcc attagtgagt gatgatttac ttccagatat ggcaatacta
    10141 gacccagagc ttgtaaaaac tgtaccaaat tttataacag cagatacagg gatggatgta
    10201 ttgacacatg ctatagaagc ttacgtatct acaaattcta atgacttttc agatgcacta
    10261 gctgaaaaag ctataaagtt agtatttaaa tacttaataa aatcatataa agatggaaat
    10321 gacatagaag ctagagaaaa aatgcacaat gcatcttgtt tagcaggtat ggcatttaac
    10381 caagcatcac ttggcataaa tcattctatt gcacatgtat taggtggaaa attccatata
    10441 ccacatggaa gaacaaactc tatattactt cctcatgtaa tagaatataa ttcatcaata
    10501 gtaggatata ctaacacaac ttactctgat acagctaaaa aatatgcaca aatagcagaa
    10561 ttagtaggac ttgggagtgc taatataaga atatctgtta gaaatttagt aaatgaaata
    10621 aagaaactac aaaaagaaat gaatatgcca actaaattga cacaatgcaa attaagttta
    10681 gaagatataa ctaatgaaga aagagaaata gcaaaacttg ctataaaaga tacttgtaca
    10741 ttaactaatc ctaaggtacc aactgaagat gatataataa acatattaaa gaacattaaa
    10801 taactacatt ttattacatg taaatataat attatacaaa tagggaaaat gtttttaaaa
    10861 atataaatta tatattgaca aatataaaac ataaaactat aatgaaagta tcacaaaggc
    10921 gtgatacttt agtagaaagc aatggagctt cttaggaata ttaatattat tcttatggag
    10981 ctccttttat tttaatcaaa tagcttttta ataaactata ttcaaagaaa gcagggaata
    11041 aatttgactg aagaatctaa gcaaagagtt attcaagaat atgttcctgg aaaacaggtt
    11101 acattagcac atatcatagc taaccctaat gaagacatat ataagaagtt aggattagta
    11161 cttgataaaa aagatgcaat aggaatactt acaataacac caagtgaagc gtcaataata
    11221 gcagcagatg ttgctacaaa ggcttcaaat gtatctttag gttttataga tagatttagt
    11281 ggttcagtag taatatcagg tgatgtaagt tctgtagaat cagcgttaaa tgatgtttta
    11341 gaagtcttag gaaatatgtt aaacttctca tctacaaaaa tcacaaggac ataataatac
    11401 aatgaagaat ataatattta tgggaaagac aggtagtggt aaaactacct tatgtcaaaa
    11461 actagaccag ttagaattaa agtataaaaa aactcaatca gtggaacttt ataataatgc
    11521 aatagacact cctggtgagt atatggaaaa tagaaaccta tataatgcct taataacaac
    11581 atcagcagat gctaaaatta tagcaatagt gtatgatgcc acacaagaag aaaattatat
    11641 agcaccaggt tttgccagta tgttttgcaa agacgtaata ggaattataa ctaaaataaa
    11701 taaagctact gaaaacgaag tcaatatagc ttatgaaaga ctatcactag caggagcaag
    11761 tcaaatcttt aaaatagaca cagttgataa tattggactt gaaaaattat ttaaatatct
    11821 agaaaaatat aaaaatatag atgaagttta aggggaaata tttttttatt caaaaatatt
    11881 attaaagtat gataaaataa aaaaatgtat aaactatttt aagaagattt aatctttaag
    11941 cgaggtagga acatggggaa aaaaatacta ttggttgatg atgaaccact gttaaggatg
    12001 gatacaaaag atatattaga aagtcatggc tatgaaataa taggcgaagc aagtgatgga
    12061 tttgaagctg ttgaaatgtg taaaaaacac aaacctgatt tagttataat ggatatagag
    12121 atgccaatac ttgatggtat aaaggcaggc aaaataatat gtaaagaaaa tctagctggt
    12181 ggagtacttt tattaacttc atttgaagat aaagaatacg tagaaaaggc taaaagtata
    12241 ggtgcttatg gatatttagt aaaaacagca agtgaaaaat cacttatccc aaccatagaa
    12301 atgtgtttaa ataaggtaaa tgaatttgaa aaaatgaaaa aagatttaga aaaagtaaat
    12361 tctaaattga atgaaagaaa attagtagaa agagcaaaag gtatacttat aagagagttt
    12421 aagatttctg aggatgaagc atacactaga ataagaaaat taagtatgga taaaagaact
    12481 acaatgatgg aaattgccaa aatgattata ataggatacg atgactaata cgataaaaaa
    12541 aatatcaact gcacaaacat acttaaataa taacgaaatt gaaaaaatta tagaagtatc
    12601 aaaatcctta gatttaatgg cttcttttta tgaagctgat gtttttatag atgtactaag
    12661 taaatttgat gatgaagcaa ttgttgtggc acatggtaga cctaaagaaa agtcaatgta
    12721 ctgtgaaaat gttataggta aaaaggcttt aattgaaaat gaaccaggag ttataaagtg
    12781 cttaaaaaca ggagaaagag taagagatat taaagcttta acacaggaat ataaactggt
    12841 caaacaaaat attcagccaa taacactaga tgacaaagtt ttagcagtac taatagttga
    12901 aaaagatata agtagtgaag ttaagaatga gtttaatgct agtgaagacg aagaaaataa
    12961 aaatttacat cagtttataa aacttattgg aaataatgac tttttagttg ataatttaaa
    13021 ttcagccata ttaatatttg ataaaaatgg aaaattaaaa attaaaaatc gtattgcaac
    13081 tgagatatac aaaaatcttg ggtttggata tgatatacaa gatatgcatt acaatgattt
    13141 agatatagaa accaacacct ttgaaaatat attaatggat gaaaattatg atgagacaaa
    13201 tgaagtttca ataggtaata tgttttttaa aataaagacc atatacatca aagatgatga
    13261 atttaaggtt tgcaaaatag tacaagatat aagtgattta aagagaaaag aagaagaatt
    13321 agttttgaaa acagtggcta taagagaagc tcatcataga gtaaaaaata atcttcatac
    13381 agtaatatct ataataaaaa aacaaagtag attatctcaa aatgatgaag taaagcagtg
    13441 tttagataac ataataaata gggtatttgc aatactttcg actcattatt tactatctaa
    13501 agaagttgat aataatatat ctatacgaga aggtatcaat ttattagtgt caaatattca
    13561 aggtggctat ttatttagta aggatataga tatacgtata actggtgaag attttaaaat
    13621 tagtggagaa aaagcaacag caatactttt ggttataaat gaaatactac aaaattgtta
    13681 tgaccatgca ttttcggata gagaacatgg tactatacaa atattaataa ataaagataa
    13741 cgactataga aatattgcta ttatagatga tggaattgga tttgatagta ataatataaa
    13801 taaaaatagc ttagggacat atataataga tagttatata acacagatgt taaaaggaaa
    13861 tatagagaga aaaagtagtg agaatggtac agaagtatta attactatac ctacacaaat
    13921 taatatttaa ataataactc atctacaaag tagtagtttt aattgagcaa agaggcttaa
    13981 ggttattaat agattaagtt aattgaatgt atttataaaa attataagta tatattcaat
    14041 ttaacaggta tttattataa ctttaagcct ctttgcttgt ttaagacata cttaaatttt
    14101 aggaggtgag aaaatgagag aagagttatt aagtgttggt attgacattg gaacaagtac
    14161 tactcagtta attttttcta aattaatagt agaaaatact gaatcaaatt tttcggtacc
    14221 tagaataagt atagtagaca aagaaattat ttataaaagc aaagtttatt tcacccctct
    14281 tatttcaaat aatgaaataa atggtcaaca aataaaagat atagttgaaa atgaatataa
    14341 aaaagcaaat atatctaaag aagatataca tacaggagca gtaataataa ctggtgaaac
    14401 agctagaaaa gaaaatgcaa atgatgtact tcatactttg agtggatttg ctggggactt
    14461 cgttgttgca acagcaggac ctgatttaga aagtataata tcaggaaaag gagctggagc
    14521 tcatatttat tcaaaagaac attcaactac aattgtaaat atagacatag gtgggggaac
    14581 tagtaacata gcctcattta aaaggggaga agtaaccgat actggatgtt tggatatagg
    14641 tggaaggctt gtaaagatag ataaaataag taaaaaaata acttatatat cacctaaaat
    14701 agataaaatc ataaaagata ataatttaaa tcttcaagta ggcacaatgg ctactgtaga
    14761 aaatacaaag ccaatagtgg atataatggt tgacttatta atggaaagca taggattaaa
    14821 gaaacagaca cctttgtttg attttataat aaccaataaa tcaataagta tagatgaaac
    14881 tattaaaaat gtttcattct ctggtggagt agctgattat atttactatg atggagaaat
    14941 aaaagattat tttgaatatg gtgacttggg aattttactt gggcaagcaa taaaaaattg
    15001 tgaatacctt aaaaaagtaa atgtagttaa atctatggaa acaataagag ccacagtggt
    15061 tggagcaggt tctcatacta ctgaagtaag tggaagtaca ataacttaca ctagcgaaaa
    15121 gtttccactt aagaacttgc cagttttaaa attatcaatt gatgaagaaa caggtacttc
    15181 aaaacaaatc agtgaagcta taaaaaataa acttaattgg tttaaactag aaaatgattt
    15241 acagaaagtt gctatagcca tgataggaaa gaaaaatcca acctttttag atatacaaga
    15301 gtatgctaaa gggtttgtag atggaatgca acaaatcata gaaaatgatt taccttttat
    15361 agtaattgtt gaaaatgata tggcaaaagt gttaggtcaa gcaatatatg cattattaaa
    15421 ttatgaaaaa gaaattgttt gtatagacag tgtcaaggtt gaaaatggag actatatcga
    15481 cattggaaaa cctatagttg aaggtaaagt tttacctgtg gtaataaaaa cattagtttt
    15541 taattaatag aaggaggtaa gtaaatgatt ttaaatacaa aattatttgg acgtacttat
    15601 cagtttaaag gcttatatga tgtaatggct aaagctaatg aagaaaaatc aggagataaa
    15661 ttagcaggtc ttgcagcaga atcagctgaa gaaagaatag cagcaaaagt tgttttatca
    15721 cacataaaat tggaggatat aagaaataat cctgcagtgg catatgaaga agatgaagta
    15781 acaagaataa tacaagatgc tgtaaatgaa aaagtttatg aagaaataaa aaattggaca
    15841 gtctctgaac ttagagaatg gctattggat tcaaatacta caagtcttga tataagaaga
    15901 atatcaagag gattaacttc tgaaatgata gcagcagttg caaaattaat gtcaaatatg
    15961 gacttagttt atgcagctaa aaagataaag gttactgctc actgtaatac aactatagga
    16021 gaacaaggaa cattatcagc aagacttcaa cctaatcatc caacagacga cccagatgga
    16081 atacttgcat cacttcttga aggtttaact tttggtatag gagatgcagt acttggatta
    16141 aatccagttg atgactctgt tgagagtgtt actaaagtat taaaaagatt tgaagaagta
    16201 aaacaaaaat atcaaatacc aactcaaaca tgtgtattgg cacacgttac tacacaaatg
    16261 gaagcaataa gaaatggtgc tcctgcagat ttaatattcc aatctatagc aggttctgaa
    16321 aaaggaaatg aagcatttgg atttaacggc gacactatag aagaagctag acagttagct
    16381 ttaaaacacg gtacagctac aggacctaac gttatgtatt ttgaaactgg acaaggttca
    16441 gaattatctt cagaagcaca tcatggaata gaccaagtta ctatggaagc tagatgctat
    16501 ggatttgcta aaaagtatga accattctta gtaaatacag ttgttgggtt tataggacct
    16561 gagtacttat atgatgctag agaagttgta agagcaggac ttgaagacca ctttatgggt
    16621 aaactacatg gcttaccaat gggtgttgat gtttgttaca ctaaccatat gaaggctgac
    16681 caaaatgata tggaaaactt agccatacta ttaacaacag caggatgtac gtattttatg
    16741 ggaatacctc atggagatga tgttatgctt aactatcaaa caacaggata ccatgaaaca
    16801 gcatcactta gagaaatgtt tggactaaca gcaataaaag aatttgatgc ttggatggaa
    16861 aagatgggat tctctaaaga tggtaagtta acagagttgg cgggagacgc atctgtatta
    16921 ttaggatagg aggtatttaa tatgaatgag aaggatttaa aagcattggt agaacaatta
    16981 gttggacaaa tggttggaga attagatact aatgttgtgt ctgagactgt taaaaaggca
    17041 actgaagttg tagtagacaa caatgcttat atagatgata taacagaagt tgatataaga
    17101 aagcaattac tagtaaaaaa tcctaaggat gcagaagctt atttagatat gaaagcaaaa
    17161 acacctgcaa gattaggtat aggaagagct ggtactagat ataaaacaga aacagtttta
    17221 agatttagag cagaccatgc tgctgcacaa gatgctgtat tctcttatgt agatgaagaa
    17281 tttataaaag gaaataatat gtttgcagtg gaaactttat gtaaagataa agatgaatac
    17341 ttaacaagac cagatttggg aagaaaattt tctccagaaa ctataaataa tataaaatct
    17401 aaatttggta caaatcaaaa ggttttaata ttagttggag atgggcttag ctcagctgca
    17461 atagaggcaa atttaaaaga ctgtgtgcca gctataaaac agggtctaaa aatgtatggt
    17521 atagattcaa gtgaaatttt atttgttaaa cattgtagag ttggtgctat ggaccactta
    17581 ggtgaagaat taggttgtga agttatatgt atgttggttg gagaaagacc aggattagtt
    17641 actgctgaat caatgtcagc atatatagct tataagccat atataggaat ggcagaagca
    17701 aaaagaacag ttatatcaaa tattcacaaa ggtggaacaa ctgcagttga agcaggtgct
    17761 catatagctg aacttataaa aactatgtta gataaaaaag cttcaggaat agaccttaaa
    17821 taaagtcgag ggagtgtaga atatgaaaaa tgatgtaatt cgtcctaata tactaggaat
    17881 taaaataatt tcaaatataa gtccagaaat ggcacaaaaa ctagaattaa aatcacatca
    17941 caaaagctta ggtttaataa ctgcagactg tgatgatgtt acgtatacag ctcttgacga
    18001 agcaactaaa gcttcagaag ttgatgttgt atatgcaaaa tcaatgtatg caggtgctgc
    18061 aaatgcttca acaaaacttg ctggtgaagt attaggaata atagcaggtc caagcccagc
    18121 agaagtaaga agtggtctaa atgcagtagt tgattttcta gagtatggag caacttttat
    18181 aagtgcaaat gatgatgatt caatagcata ctatgctcac tgcgtatcaa gaacaggtac
    18241 atacctttca gaggtagcag gaataagaga aggtgaagca ttagcttatt tagtagcacc
    18301 accccttgaa gcaatgtatg cactagatgc agcaatgaaa gctgcagatg ttaagatgtg
    18361 tgaattattt gcacctccaa ctgaaacaaa ctttggtgga gcattactta caggttctca
    18421 atcggcttgt aaagcagcat gtgatgcatt tgcagaagca gtaaaatcag ttgcagacaa
    18481 tccaacagga ttttaaggat agttagatgg atgctatagg cttaatagaa gtaataggat
    18541 atgttgctgc aatagaagct tctgatgctt gtttaaaatc tgcaaatgtt aatattgtaa
    18601 gagttgataa ggttggggca ggtattgtta ctttaacaat atgtggagat gtcggtgctg
    18661 taaaagctgc tttagaagca ggtgaaatag cagcttccag agttggtaca ttaagaacat
    18721 cacatattat accaagaatc cataatgaag taacaaatgc tttattcaaa actaaagaac
    18781 ctaaagtctg tgaagtttca gaagataaag aaaaaaactt tgagattaca ttggaaataa
    18841 ctgataatgt atcgaaaaat ttatcagaaa aagaaaatgt aattttagat gtcaatgaaa
    18901 atagcggaaa tactgaaaat ttatatagtg atatagataa aacaaacaaa aaaattgaaa
    18961 cagttgatga tagtttaaca gaagctaatg aggaaaaaat agaaaacact aatgataaag
    19021 aagtattgga acaagttaaa atagaaaatt ccgattttga tagagatgac ctatcaaaga
    19081 acagtgtaaa aaaacttaaa gctatggcta agaaattaga ttcttcaata acttataaag
    19141 atttaaatgt tcttaaaaaa gaagaattaa taaacttagt taaaaagctt agcagaaggg
    19201 atagatagtt atgacacttt tagataaaga tttagtatct atacaagaag tcagaactct
    19261 tgtaagaaga tctaaagtgg ctcaagagaa actttctaaa atgagtcaag agcaaataga
    19321 taaaatagta aaatctgttg tagatgcagc atttaataat gctactaaat tagctaaaat
    19381 ggcaaatgaa gaaactggat ttggaaaatg ggaagataaa gtaattaaaa atacttttgc
    19441 atctatggga gtttatgaaa ctttaaagaa cacaaaaact attggaataa ttggtgaaga
    19501 taaagaagcc aatacgatag atatagctac accagttgga gttgttgcag gtcttatacc
    19561 ttcaacaaac ccaacatcaa cagtaatata caaaactata atatctctaa aagcaggaaa
    19621 tactatagtt ttctctccac atccaaatgc aaaaaactgc atacttgaaa cagttaatat
    19681 aataagtgag gctgctataa aggctggagc tccagaggat tcaatattat gtataactaa
    19741 tccaagtata gttggaacca atgaattgat gaaacataaa gatacaaact taatactagc
    19801 aactggagga gaagcaatgg ttagagctgc ttactcttca ggaacaccag ctattggagt
    19861 tggaccagga aatggaccag catttataga taaatcagca aatgttaaat tagcaatcaa
    19921 aagaataata gattctaaaa cttttgataa tggtgttata tgtgcatcag aacaatctat
    19981 agtaatagaa aaatctatgg aagaaactgt tttaaaagag cttaaaaatc aaggtgcata
    20041 tgttttaaat gaagaggaaa gtgaaagatt agcaaaattc atattaagag ctaatggtac
    20101 tatgaatcca caaatagttg gtaaaagtgt tgaggtaata gcaaatttag cagacttaac
    20161 aaatgtacct aaaaatacaa aagttctagt tgcaagagaa aataaagttg gtcacaatgt
    20221 agcctattca agagaaaaac ttacaccaat actagcgttc tatgttgaag aaaatgtaga
    20281 agctattatg aacagatgca gagaaatatt gttaaatgaa ggtgcaggtc atacattctc
    20341 aatgcatgta aatgatgaag aattggtaaa aagatttgcc cttaatatgc cagtatcaag
    20401 aatacttgtg aatactcctt cagcacttgg aggaataggt gcatcaacaa accttgtacc
    20461 agcactaaca cttggttgtg gtgcaatagg tggaagttca acttcaaaca atataggacc
    20521 acttgattta ataaatgtta aaagagtagc ttatggaatc aaagaaatag aagaacttgg
    20581 aagagataaa tctcaagttg aaaaatcaat gtttgactca atggataaag aagaattaat
    20641 aaatcttctt gttcaaaaac tagtcaaaga actagcataa ctaaaataaa caatgaaact
    20701 tatagctatc actataaatt tctaaaaata taaactataa aaaactataa aattaaattt
    20761 ttggaggata agattatggc aagtgcaaac gcattaggaa tgatagaaac taaaggatta
    20821 gtaggagcaa tagaagcagc agatgcaatg gttaaagctg caaatgtaca attagtagga
    20881 aaagagcaag taggtggagg attagttact gtaatggtaa gaggagatgt tggagctgtt
    20941 aaagctgcta cagatgcagg agctgctgct gccgagagag ttggagaact aatatcagtt
    21001 catgttatac caagaccaca ctttgaagta gatgctatat taccaaaggt atcagctgag
    21061 taatataaga ataattataa aatttaaatt ttgattgaaa aagaggctat ctcaaaaatg
    21121 agtcttacta ataaactcaa tgtattttga ggtagtcttt tttatacatc taataggaga
    21181 tgtgataaaa tgggtgtttt aacagaaagc gaagtaaaaa gaaaaattgg aaaaaacaaa
    21241 aataatataa ctgaatttta tatagaaaaa ggtcaaataa taactccttc tgcaaaatct
    21301 tatttagctg agagaagtat tgagcttaaa tatgttgata aactttcaga tgtaaacaat
    21361 gaacctaaag aagttgtaaa agaagtagtt aaagagaaaa aatatagata tactactgta
    21421 tttggcgcta aattggatga aaaaccagaa catatgactc aattaaatgg aaatctttta
    21481 gtttttaaag accataaaag aataatattg agaggaaaaa tagattctct tgaatcagaa
    21541 atactaaagg tacagatact ttgtcagaaa ctagatatgc caaagcttgt taaggaattg
    21601 caagaagtat tagactttgt aagaaatata atgagatgtg aagttttaga tgaaaagcta
    21661 ggggacttta aacttttagg attaactcct caagaactta gagaacaatc tcactatcct
    21721 aaaaaatact ttggtatagg acatgagttc ccaagttatg aaatgggaga agtagtagta
    21781 gctataaata gtataagaag taatacacga gaggttgaac ttgcagccta tgaagcattt
    21841 aagggtgagt atggaaatgt tcacagagaa gatttaataa aggcacttaa tagattatcg
    21901 tcagtatttt ggataatgat atataaggtt agaactaata agtataagta gttggaggac
    21961 ataatggaga atctattaaa tgaaatagtg gaagaagtaa taactagagt taaaaaagaa
    22021 gcatttatag aagttgaagc atcaggaaga cacgttcact taagtagaga agatgtcgat
    22081 aaattatttg gagaaggata tactcttaca aaactaaaag acctatctca accaggtcaa
    22141 tatgcatgta aggaaagagt tacaataact ggtcctaaag gaagtataaa aaatgtagtt
    22201 gtacttggac catgtagaaa tgaaactcag gtagaaatat ctttaacaga tggttcaact
    22261 cttggactaa aagctcctat aaaacaaagt ggagacttag aaggtacttt aagtattaaa
    22321 atatcaacac aatatggtga agttgaactt gataaggggt taatggttgc taaaagacat
    22381 atacacatga ctcctaaaga tgctgaaaaa ttcaatgtat gtgataaaga gatagttgaa
    22441 gctaaggtga tgggtcaaag acctttaaca tttgatgatg tagtaataag agtgagtgac
    22501 agttttaaaa cttatatgca tatagactat gatgaagcca atgcatgtgg ttattcaaaa
    22561 ggaactgttg ctaaaataat aaagaaagcg taaatttaac ctactatata aattacctct
    22621 agggaggatt taatatgaat tatgataacc ttgtagattt aataacggaa gaaatctata
    22681 aaaaaataaa tagtaatgaa ataaaaatat ctaataaacc taaagctgtg attgtatttg
    22741 aacaagataa caataaattt aacttattaa aagatgaatt tgaaatagta gaatttgata
    22801 aaagtataag ggaatgtgaa atagtgatag tttcaagact ttgtatgagg gggctatcga
    22861 atatagcact tggaaattca acaagtgatg aagaaagatt tatactaaaa atgattatga
    22921 agggcaaaga agtatatgtc ctagatgaag gaattgaata caaaaaatat aaggatactg
    22981 caccaaaagc attatataag aaatttatgt catttgaaga tgaaatatgt aaatttggtg
    23041 tagaaattat aaaagactta aatgttataa ctaaaaataa gttaaatata aagaaagaac
    23101 tagaaagtac tagaggttct aaaaaagaag atacaaaagt tttagataaa gatgttttaa
    23161 atttaatcag tgtagaaagt gtaaaaatag ataatgaatt tagtttagat ttaaggaata
    23221 aaaaacttat atcggaagct gacttaagaa aaccaactat aaatggagtt aaaaacatat
    23281 tagttaataa aaaaagtata ataacaccat tagctgtaga ttttatgaga atacaccatc
    23341 tcaaactaaa gaaagtgtaa ggaggtaatt ttatggatat aggaaaagtt gtaggtaatg
    23401 tttgggctac aagaaaagat gaaaaattat gtggccaaaa acttttagtt gtaaaaattt
    23461 taaaaactaa agacacatac aaggaaggct tcatggttgc agccgataat gcaggagcag
    23521 gaaatggaga cttagttctt atatccaaag gttcctcagc tagagagtcg attgaaaaat
    23581 ctcatgttcc ggtagatgca actatagttg gagtagtcga ttcgtttgag gtgtttgatg
    23641 aataaaagta taggagctat agagtttaaa agcattgcta aaggtataga agtatccaat
    23701 gaaatgataa agaaatcttc agttgatgta ttgtacttaa aaagtatttg cccaggtaaa
    23761 tttctaataa tagtaggtgg agaaacttct tatataaatg aatgtgtaga ttatggaata
    23821 aaacttggag aaggatatat tgtagataac ttcgtaataa atgcaatttc acaagaaata
    23881 ttagatggat ttaaaaataa gtatcaaaag ctagatagta tagtctctat aggcgttgtt
    23941 gaaagtagta aagtatgcgc tggaataaaa atgcttgata agactttaaa atctggcgac
    24001 ttagtattag taaaacttca attatctttt gctataggtg gcaaattagt atatatagta
    24061 gcaggagatt taagtagttt agaatatgca ttaaaagaaa gtgaaaatgt agtaagagaa
    24121 aaagaagtaa tatataagac tgtaatacca tcagtagaca gtcaaataat aaaaagtcta
    24181 ataaaataat ggggggaatt atgagtataa acgaaattat aatctatgta atggtagtct
    24241 tcatggtact tggagctata gataaatgta taggtaacaa atttggtctt ggagaacaat
    24301 ttgaagaagg tataatggca atggggtcac ttgcagttgc aatggttggt gttatatgtt
    24361 tagcaccagt tcttgctgat gtattaagac caattgtagt accagtattt aatatacttg
    24421 gagctgaccc agctatgttt gctggaagtt tattagctaa tgatatgggt ggtgcaccac
    24481 ttgcacttga acttgcgaaa gaccctaatg ctgggttatt tggtggatta atagtagggg
    24541 ctatgatggg acctacaata gtatttataa taccagttgc gcttggaata attgaaaaag
    24601 aagaccaaaa attcttagca acaggaatac ttgcaggtat aataacaata cctattggag
    24661 cattcgttgg tggattagtt gcaggattcg aggtaatgat ggttcttaga aacttagtgc
    24721 caataataat atttgcagta gttatagcac ttggattatt aaaattcgaa aatgctatga
    24781 taaaaggatt cacatacttt ggaaaaggtg tagtaatagt tataacttta ggacttgctg
    24841 ctgccatagt tgaagcttta acaggaatag ttgtaatacc tggaatgaaa ccaatcagtg
    24901 aaggtataga aatagttgga gatatagcaa tagttttagc aggagcattc ccattagtgt
    24961 ttgttataac aaaggtattt aataaaccat taatggcttt aggaaaagtt cttggaatga
    25021 atgatgtatc agcagcaggc cttgttgcaa gtttagctaa ctgtatacca atgtttggta
    25081 tgatgaaaga tatggacaat agaggaaaaa taataaatgt agcattctct gtatcagcag
    25141 cattcgtatt tggagaccac ttaggattta cggcaggatt taattctaat atgataacac
    25201 caatgatagt tgctaaatta gttggtggta taacagcagt attgttagct atggttatcg
    25261 caaataagac actaaagaag gagaatgtat aagatggata tatcaaatat agataaaaat
    25321 ttaattgaaa cattagtaag acaaataata gaagaaaaaa taagtggaac taaagacaca
    25381 gtagattttg ttagaaataa agacattagt ggaataactt ctataaagtt accaactgta
    25441 aaagttagtg aatctgatag attagataca gggaatccaa gtgatgtagt ttacacaaaa
    25501 gatttattta cattagagga aagcccaaga ttaggttgtg gtatgatgga aatgaaagaa
    25561 acaacttttg attggactct aaactatgat gaaatagatt atgtaataga tggaacttta
    25621 gatataataa tagatggaag aaaggtttct gcttcttcag gtgaattaat atttatacca
    25681 aaaggaagta agatacaatt ttctgtacct gattatgcta gatttatata tgtaacatat
    25741 ccggcagatt gggcatctca aaattaataa aaatcaagtt aagttaataa taatcaaata
    25801 aataatttaa aatacaaact cttaaataag cacacatatt taaaaaagct atgaattaaa
    25861 attcatagct ttttttataa agaatgttat attttagacg tattttttct atatttactg
    25921 ggagacatat tatatttttt attaaataat ctaataaaat gctctacatt tggataacca
    25981 atactatttt actgataatc ttttccttat cactaatctt acattttaaa atattatttt
    26041 tactcgtaat attatcataa gaatttgttt cttatttaaa actaattaga attataaagt
    26101 ttattgccat gttattaata aaataaatcc agttaaatcg agtgctagtt aaagtaataa
    26161 gaaactttta caatggtttc ctttatcgat tgaaataaaa gttaatatgg tataatatct
    26221 aaagagagaa tcttatcaat tagaatatgc atagaaacaa gtggcttaaa aggagacgtt
    26281 atatggaaga gatagtaaag aaggaacaag caagtatgaa tgatattttt ttgtatctta
    26341 aagctaaatc aaactcatta tataaatttg taatattata taatgagttt atcaataaac
    26401 ctaaagatta tggaactggg gaacttataa acatggttga agtacatatg ctaacagcga
    26461 ttgaagaaaa tccaggaata agtccaactc agttagctaa aatatggaac agaactaaag
    26521 gtgcaatatc tcaaactata tctaaattgg agaaaaaggg ttatataact aaagaaaaga
    26581 aagaaggaaa tgcaaaaact atattactat atccaaccac atcaggagta gagttaagta
    26641 aggcacataa actatatgac accatagatg ttacaagaac atttgaatac ttaatcaagg
    26701 agtgtacatt agaggagata gatagttttt ataaagtaat tgatttgttt attaatttat
    26761 tagaagaatc tccgcaggaa aataatgatt ctgaaaaagt gtagctgaga ttgaagttga
    26821 ataaagagaa atagatatta tagaagtagt acttaagtac acattatata aaaatataat
    26881 aggtatagga ttaaaaagtt tagtggttta aagtttaacc actaaacttt tttaatgtct
    26941 ataatctact attatttaat ttactaaaaa taaaaaaact cattatatta agataaaaat
    27001 agaaaaataa ctagaaaaat actataatat atcagcttaa tacagaaaaa agttaaaaaa
    27061 tttgagaaaa ataaaaatga aaacgtttta tattgacata gagaaaaagt agtgatacca
    27121 tttaaatata gtttagtagc taaactatat ttaaattata ataactattt tagggggatg
    27181 ttatgaattt aaaacaatta agtgaaaaat acgaagaata tgtaattcaa atgagaagag
    27241 aatttcataa gtatccagaa ttatctttta aagaaataag aacaacccaa agaataaggg
    27301 aagaattaca aaaaataggg gtcccttttg tatctattga accaaatatc gtaatagcta
    27361 ctatacaagg caaaaatgaa ggtaagacaa ttgcaattag aggagatata gatgcgcttc
    27421 caatgcaaga agattcagat gttgaataca aatcagaata tgatggaata atgcattctt
    27481 gtggacatga tgctcatgga gctatgttac taggacttgc acatatgcta aatgatataa
    27541 gaaaagattt aaaaggtaga gtattattag tttttcaagc agctgaagaa gttggtggtg
    27601 gacacaagga gataatacag tactttaagg aaaatggagg accagataga ttaatagcta
    27661 ctcatgtttg gtccgatatt gaagctggta aaatttctgt tgaaccagga cctcgtatgg
    27721 ctggtgggtc tggttggaga atagacataa caggtagagg aggtcatggt tctagaccag
    27781 atcaaagcat agatcctata aagccagctt gtgatatagt tttaaaagta tcttctatac
    27841 cagttaatca tataagtgta ctagaacaat gtgttgtaca tgcatgtgca atacatggag
    27901 gaactacaat aggaaatgtt attccaaaca atgcagaggt aataggtgga tatagatatt
    27961 ttactgaaga atcaggcaaa aaagtgtttg acattataaa gcaaatgagt aatggtgttg
    28021 gacaagttta tggagtggat gtgaaggtta ctcatactgg aggcatagga cctgttatca
    28081 atgatgaaga agcagttaag atggctaaag aaatagtttc taatatagat ggattggagt
    28141 tagattctta tgagccaata tgtgcatcag attgttatgg tgaaatatta aaagagtatt
    28201 ctggctttta ttgttatcta ggggtaggta acaaagaaaa aggaataata tatgcacaac
    28261 accatcctaa gtacaatata gatgaagata cattaaagtt aggttgcgaa tttatggcta
    28321 aatatgcagt tgaattttta aataaaaata ataactaaaa taaatcgaat tttataaatg
    28381 ggggggatga aaatgcaagg tgcattaaag gaaaataaga aaaaaagtgg tggaggattt
    28441 ttaggatgga ttgaaaaagt tgggaataaa atgccacatc ctctagcatt atttacatat
    28501 attacattga tagtagtagc attatctgca atagcaagct ttttaggttt ttcagcaaca
    28561 agtcctgcta caggaaagtt aataaaagtt atgaacttaa taagtgcaaa aggtttagta
    28621 ctatttatgc aagagtttgt taagaacttc caaaacttcc ctgtgcttgg agtagtttta
    28681 gttatggcag ttgccacagg agtttgtgaa aagacaggat ttttttcaac tgccataaaa
    28741 atgagtttat caaaagttaa ggggaatgcg gttgtattta taatagcttt tataggagta
    28801 tttgctaatc aagctggtga tgctgctttt gtattagtac caactcttgc aggagcaata
    28861 ttttatggat taaatagaaa tccattagct ggtatattct gtggatttgc atcagttggt
    28921 ggtggatttg caacagctgt aattcctggg ggatgggacg ttacgctaac gccaataact
    28981 gtatcagctg ctcaaactat tcaacctgca tttgatatga cattattagc tagttattat
    29041 gcactatttg tatctgcgtt tatagtagct acagtatcaa caatagtaac ggttaaattt
    29101 atagaaccta aattaggtaa gtatactggt aaaccagaag gtatagatga taatcaaggt
    29161 tttgaggtca caaaagaaca agcaaaagca gcaaagttag caggttttac agttttagct
    29221 tatgtagcgt tattaattgc tttgtgtata ccagctaata gttttttaag aagtccagaa
    29281 ggttctttaa tatttggagc accacttatg tcttgcttac agttttttat agtaatacta
    29341 ttcttcttgc cagggattgt gtatggaatg agggttggta aaatcaagac tgtaaaagac
    29401 ttaaatgata tattatgtca atcaatggca gcaatggcac cgtttatcgt acttgcaata
    29461 gttataggtc aattcttaac gctattctca gtgtctaatt tagcacaggt actagctata
    29521 aaaggtggag cattactcaa ctcattgcca ctaccaccac aagctataat actgctattc
    29581 ttagtactag tagcgtttat aaatatattt gttataagcg gaagtacaaa atggatgata
    29641 tttggaccag tatttgttcc aatgttaatg caattaaaca tacatccagc atttactcag
    29701 tttgtatata gattaggaga ttctgtaacc aatcatttat caccattaaa tgcattcttt
    29761 ataatacttt tagcaacagt ccaaaaatac gataaaaatg caggtatggg aactatattc
    29821 tcagctatga taccttacac tataggttac tttgtagtac ttgcagtttt agtagtagtt
    29881 tggatgacaa taggtatacc agtaggaata ggtggacaag tttggttata aaacatattt
    29941 aataattttg ggaggaaaga aaatggaaac aactgaaata agatacaatt ttgatgaagt
    30001 aatagataga agtggaacaa actgtcttaa atatgatctt aagagtcaat ttatgccaga
    30061 atctccagaa gatgcactac cactatgggt agcagatatg gattttgcat gtgcccctga
    30121 gataattaat gcaatgcatg aaagaataga cagaaaaata tttggatata gctctcaaca
    30181 aactgatgat tattatgaag ctgtatgtgg atggtttaag agaagatttg attggaaaat
    30241 agaaaaagag aatatattct ttagcccagg ggttgtgcct gcgttaggat ttttcacaca
    30301 aatattaaca caaaagggtg atggtatcat aatagaaaag cctgtatact atccttttga
    30361 agataaaata actaataatg aaagaaaagt agttaatagt cctttaaagt ttgaagatgg
    30421 tcattattct atggattatg atggatttga agaattagct aaagatgaaa ataataaggt
    30481 gtttatatta tgtagtcctc ataatccagt tggtagagta tggaaaaaag aagagcttag
    30541 aaaaatagta gacatatgta agaaatatga tttgtggata atatctgatg aaatacattg
    30601 tgatattatt cgtaaaggtg aaaagcatat accattagaa gtagcatgtc ctgattataa
    30661 agatagaata ataacttgta cagctccaag taagagtttt aacttagctg gtatgcaatt
    30721 atcaaatata gtaatcaata ataaggaatt acaaaaaaaa tggatgcttg aaacagattc
    30781 taaattagct atattcctac ctaatccatt cgcaatagtt gctactaaag ttgcttataa
    30841 tgattgtgaa gattggatga atcaagtaaa tgagtatata gatggtaata ttaaatatgt
    30901 agaagatttt gttaatgagt atatgcctaa agtaaagata ataaaatctc aaggaactta
    30961 cttagtttgg ttagacttta gagggtatgg attagatgca aaacagcttg aatatgtaat
    31021 gcttaacaag gcgaaggtat tgctagatga aggatacata ttcggagaag aaggagcagg
    31081 atttgaaaga ataaatgttg cttcacctag aacaattata gaagagtgta tgaatagaat
    31141 aaaatgtgca tttgaaggat tataatagta atatctaaat aatatatttg tgtattttaa
    31201 aaaaagacta tgctaaaaaa atatttcaaa gcatagtctt ttaaagtttt atgatgagtt
    31261 taaaaaattt caaaacgtta ctattcactt atatgtttat attagaaata attccacaat
    31321 atactcattt tctaaagttg cacttttata caccttgctt caccaatatc atgtgtaacc
    31381 aaaatagtag acttattctc attcttaata atactataaa catcatcaca aactaataat
    31441 ctagtctgat aatcaagagc tgaaaaaggt tcatctaaaa gtagaatatc tggatttaca
    31501 gaaagtgttc taataagcgc aactctttgt ctcatacctc cagataattc ttttggatac
    31561 atacttctaa aatcccataa tccataagtc tttaaaaggc tttcaactct atctaaagat
    31621 tttttatctt ttttgccttg aatttccaat ccaatagtta tattatccat tatatttctc
    31681 cattcaagca aattatcttt ttgaaacata tatccaatat ttccagtaat ctttatatca
    31741 ccactagtcg gttttaaaag attagtaaga atatttaata ttgtagattt accacttcca
    31801 gaaggaccaa gcaaagttaa tatttctcct tctttcaagt taaaattgac atcctttaat
    31861 acttcaagct cgccttcctt agtgtagaag ttcatattta tatttttaac ttctactatg
    31921 ttgtcattca ttatgccacc ccctataact tttagctcgt atatattatg ttattaacaa
    31981 tatgacctat ttgtaccatt tttcataaat ataatataaa tattttgttt atttacgtat
    32041 tttcttagta acttgtacgt ataaattata gataaataaa ttataaggta g
//
LOCUS       DAELLV010000017        32060 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.017, whole genome shotgun sequence.
ACCESSION   DAELLV010000017 DAELLV010000000
VERSION     DAELLV010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32060
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.017"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            222..1559
                     /locus_tag="KPD58_000711"
     CDS             222..1559
                     /locus_tag="KPD58_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG0264497.1"
                     /translation="MGTYSLISTSINFILILINIFIAFFLYTLTSYFIDFRNHWIFKI
                     ILILGYSIISQMIIWLSDPVNILFTLFGYFILLIICSKSKLIPIISVVMILYPIIIGL
                     NFLFINNPIYEELIHSKEYILILSIVQIISALIKLLFWFLIHIFLKNRLVNIKQYLSN
                     KIWIFIDMVCISSFLSILIAIILPPTNLIPMGSNKNIILGNLPYGTYLIILSAILANI
                     GVILLLQPLIENVKMKIEKQANHLKEKYYNLLENQQIQIRKMRHDMNNHFQMIESYLE
                     IEDIAGAKKYFNQLKIGIGTLSGKQFCKNQALNSILNLRHTSLEENGVDVHFNIDIDN
                     HLGIEPIDLCTIFSNSLDNAIEASLKIPDLSNRKVVLKARCEKEYFSYLLTNNKVNPI
                     NRKQELFISDKKDSNSHGYGIENIREIVNKYNGKLDISYTESEFSLFLYIRLF"
     gene            complement(1895..2464)
                     /locus_tag="KPD58_000712"
     CDS             complement(1895..2464)
                     /locus_tag="KPD58_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HBG0264498.1"
                     /translation="MKKKLEDMTLEELWNLFPIFLTTHKECWKNWFKEEQYRLTGILP
                     MQKIVRISHIGSTSVSTIYAKPIVDILIEIIPDYNMKDIKDLIESNGYICMSESSSRL
                     SLNKGYTVDGFEERVFHLHIRYVGDNDELFFRDYLIDHPDVAKEYEKLKLDLWKKYEH
                     NRDAYTDSKLDFVREFTEKAKVLYSNRYN"
     gene            2867..3439
                     /locus_tag="KPD58_000713"
     CDS             2867..3439
                     /locus_tag="KPD58_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HBG0264499.1"
                     /translation="MEYIINPCSGIKIKITRGQKIKIVDIEGGQVVDFFAEMKDDSTE
                     FISPGVTIDCNESLRLQVGNLIYSNKYRPMFKILYDDVREHDLLHPCCRPEMYDFFYH
                     NGENHPNCFDNINRTLGTSHQIIHPINFFMYTKVNTDGSIEVCEPLSKASDKIILEAL
                     ENMTLGIAACSVSESSCNSRKCTSIKILVD"
     gene            complement(4118..5020)
                     /locus_tag="KPD58_000714"
     CDS             complement(4118..5020)
                     /locus_tag="KPD58_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HBG0264500.1"
                     /translation="MLPQDYYGLIVILGVILGVMGAFYNWFTLYVQSLYKKIPKIGTF
                     GKLLIPFIMAGILGLVMPDVLGSGHNLVEELTSHKYIFGMVVLIFVIRFIFSAVSFGS
                     GAPGGIFFPLLVLGAFIGGIFGMVGVKVFGMNPDYVNNFVLLAMAGYFTAIVRAPLTG
                     IILIFEMTGSVSQMLSLSVISIVAYIVATLMKSKPIYESLLERLLEKNGEPVHKERGE
                     KILDQFSVMFGSPVANKAIQDVQWPENCLLVAIQRGGEEIIPRGKTILRPSDVIVTMT
                     DERDSGAVYDKMENLCKEHTAHIF"
     gene            complement(5147..5266)
                     /locus_tag="KPD58_000715"
                     /pseudo
     CDS             complement(5147..5266)
                     /locus_tag="KPD58_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5813..6475)
                     /locus_tag="KPD58_000716"
                     /pseudo
     CDS             complement(5813..6475)
                     /locus_tag="KPD58_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440163.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
     gene            complement(6635..7171)
                     /locus_tag="KPD58_000717"
     CDS             complement(6635..7171)
                     /locus_tag="KPD58_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HBG0264501.1"
                     /translation="MIGLKCGMVKLLEHQMIWDKSAKDVIILLKSIWDKTAIDIQHIG
                     STSIPSISAKPIIDIVVGVASLEEAKLYLERLEQCGIVFRGQDVPKQLLFAMGDFEKN
                     TRTHHIHVVEWNSVAWNNYINFRDYLNTFTDKAKLYDSHKQKLALQFEDNRKIYTQRK
                     QELINILLEEARLWRINT"
     gene            complement(7244..7657)
                     /locus_tag="KPD58_000718"
     CDS             complement(7244..7657)
                     /locus_tag="KPD58_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBG0264502.1"
                     /translation="MKMPKESIDTIMFAPCGMNCMVCYKHCYHKKPCAGCLNGDTGKP
                     EHCRKCKIKDCVCQKGLFYCFECFNFPCKLIKNLEKSYNKRYQASLMENSKFVQQYGL
                     EKFMQKQKERYTCSKCGGIISIHDRECSECQEKVK"
     gene            8150..8788
                     /locus_tag="KPD58_000719"
     CDS             8150..8788
                     /locus_tag="KPD58_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HBG0264503.1"
                     /translation="MDEKILELLQEMQGNISNMQGSISGIEGTISDMQSNINGIEGTI
                     SDMQSNINGIEGTISSMQGNINGIENTISGMQGTIGDMQGSISGMNGTISDMQGSISS
                     MDGTINSMQGSITDMQVEIKEVNSRLDNMEPDIKDLKTGQVETSSRLDTIQSDVTEIK
                     SDVKINRNTLDTVIKHTSSLVKDTSDIKNDLNNIEIVTSKNSNDIAKLKSVK"
     gene            complement(9396..9575)
                     /locus_tag="KPD58_000720"
     CDS             complement(9396..9575)
                     /locus_tag="KPD58_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG0264504.1"
                     /translation="MLQTGKQGADKKYLDNMFFVKGYVEELLFLDKSYDIVFSCIVFH
                     HCTNVQNIFCIFWKW"
     gene            complement(9801..10334)
                     /locus_tag="KPD58_000721"
     CDS             complement(9801..10334)
                     /locus_tag="KPD58_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBG0264505.1"
                     /translation="MQYISYYHSPIGNILLASDNIGLTGLWFENQKYYAYNLDQEHQE
                     KELPIFEQTKKWLDIYFSGKEPNFTPSLHMVGTPFQTTVWKILQQIPYGKTITYGEIA
                     YKVAQQKGISKMSAQAIGGAVGHNGISIIVPCHRVVGTNGSLTGYAGGIDRKIKLLSL
                     EKVSVQHYFIPKKGSAL"
     gene            10651..11001
                     /locus_tag="KPD58_000722"
     CDS             10651..11001
                     /locus_tag="KPD58_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264506.1"
                     /translation="MGKVRIGCKKEKYFYVRNPVKSLVDRITENEQFAIYHFTHKYNW
                     RNKVFSILQRLIRLEKSKNELYMYEALVSVSFLWLIMIKNIVLPPIKHKNIINIHMQK
                     ILRYIEKYYCFTLY"
     gene            complement(11877..12449)
                     /locus_tag="KPD58_000723"
     CDS             complement(11877..12449)
                     /locus_tag="KPD58_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBG0264507.1"
                     /translation="MWIYQKTLEHPVNIRQADPRMAKYIMTQLGGPNGELAAATRYLQ
                     QRYTMPTGKSRALLTDIGTEEMAHVEIISSVLYQLIGNCTPEELKAAGLGSNYANFGH
                     GLQPVDSNGVNFTTSYINVFGDSVTDLHEDMAAEQKALATYYQLINLTDDPDLKDILR
                     FLGEREVVHYQRFGEALMDVYEFTECKHQF"
     gene            complement(12511..12774)
                     /locus_tag="KPD58_000724"
     CDS             complement(12511..12774)
                     /locus_tag="KPD58_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HBG0264508.1"
                     /translation="MSLNRRELLERISEYQFACIELNLYLDNNPRDKKALDSYNRYCD
                     KFTQAVCDYESKYGALTNFGYESSEYPWSWISEPWPWDKSFYK"
     gene            complement(12774..12995)
                     /locus_tag="KPD58_000725"
     CDS             complement(12774..12995)
                     /locus_tag="KPD58_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HBG0264509.1"
                     /translation="MGYLKEDKQDYCCEFARPYINRQIYDDTYNPKTALKRGTLFPEL
                     DLIESNNYNDWLYANPKNRVRAKKGGNMY"
     gene            complement(13491..14027)
                     /locus_tag="KPD58_000726"
     CDS             complement(13491..14027)
                     /locus_tag="KPD58_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HBG0264510.1"
                     /translation="MEYKSPVYGIIAVPIEKIESNDYNPNAVAPPEMQLLYKSIKEDG
                     YTMPIVCYYNEEKDKYIIVDGFHRYRVMLENEDIYDRENGLLPVSVIDKSLDERMAST
                     IRHNRARGSHNVDLMSKIITELCEIGKSDEWISKHLGMEVDEILRLKQITGLAYLFKD
                     KEFGNAWIPFGSDIEEEN"
     gene            complement(14045..15358)
                     /locus_tag="KPD58_000727"
     CDS             complement(14045..15358)
                     /locus_tag="KPD58_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HBG0264511.1"
                     /translation="MDKRYLESDVFEAFQERLSYLFNEFDYIYVSFSGGKDSGLLLNL
                     VLDYKRKNNISSKIGVFHQDFEAQYSLTTEYVEKVFEDNIEDIEPYWVCLPMATRTAV
                     GNYEMYWYPWDDEKEELWVREMPVKEYIINQKNNPFTYYKYKMRQESLAKRFGRWIRD
                     IHEGAKVVCLLGTRAAESLQRYCSIVNKQHGYKGKCWITKEFKNVWTASPIYDWEIED
                     IWLAHYKFDYDYNKIYDLFYKAGVAPRKMRVASPFNDSAKESLNLYRILDPDVWVKLV
                     ARVQGVNFTSIYGKTKAMGYRSVKLPYGHTWKSFTYFLLDTLPENIRNSYIKKFSTSI
                     EFWQNTGGGLSEIVIRELLRKGYKIELNGVSNHTKDKKSRVIFLDDIPDDTDDIKFTK
                     DIPSWKRMCYCILKNDHMCRFMGFGPTKEQRIRTEYIKKKYEKIH"
     gene            15713..16153
                     /locus_tag="KPD58_000728"
     CDS             15713..16153
                     /locus_tag="KPD58_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBG0264512.1"
                     /translation="MYERMLDKSNKPCLSQIIEYIGKDGYARLCKLETYLKTQYNLSR
                     ELRFPFGNNYGWGYKYSHKSFHLFYAFFEKGAFTVLIQIGDKQVPAVEKTLVALTPKA
                     KELWVTRYPCGNNGGWIKYRILEDADLLDIIQYIRAKKSPPKQN"
     gene            complement(16338..16526)
                     /locus_tag="KPD58_000729"
     CDS             complement(16338..16526)
                     /locus_tag="KPD58_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264513.1"
                     /translation="MNKNNNLVIICMFIGMILGMAIGCAIGISKGNVGITMCYGLIFG
                     MIIGICIGTVIKNSNKKE"
     gene            17467..17862
                     /locus_tag="KPD58_000730"
     CDS             17467..17862
                     /locus_tag="KPD58_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264514.1"
                     /translation="MNILKMKKTGIFLGGVLFGTVGVKILSSDCVKKTCTRATAGALK
                     VKDSVLETTTKIQENVEDILAEAKEINEQEDLSTSKVNVVQDTLKCEQEVLKTETIKT
                     VEDTNSNSIKCEEIKDNVIEEKNSTCTTE"
     gene            17905..20004
                     /locus_tag="KPD58_000731"
     CDS             17905..20004
                     /locus_tag="KPD58_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBG0264515.1"
                     /translation="MNFYIKHEIRGRIRFDLQLGKLNNKQSDTLLYYLTSINGITKAK
                     VYDRTGDAVIYYTGEREYIIREITKFSFNNSKIENLVPEHTSRELNNYYRDKLANKLL
                     VKSISKIFFPKSLRFFLIGLHSIKYFKKGLSSLLNKKIEVSVLDATAIGISIFRKDIN
                     TAGSVMFLLGIGELLEEWTRKKSIDDLAQSMSLNIEKVWLRKGDTEILIPISEIKEGD
                     LVSVTMGNMIPLDGVIVSGETMVNQASLTGESLAVNKKEGSYIYAGTVIEQGNIVMCV
                     KEKAGTTRFQKIVTMIEESEKLKSSVESKFEHLADTLVPYSFLGSILTYAITRNPIKS
                     LSILMVDFSCALKLSIPISVLSAMRECNNNNITVKGGKFLEGVACADTIVFDKTGTLT
                     KAQPTVSDIITFQNYNKEDMLRLAACLEEHFPHSIANAVVYEAEKQGLSHKEMHTEVE
                     YIIAHGISTKVDDKKVVIGSKHFVFEDEKCTIPDGEEDKYNNLSDEYSHLFMAISGKL
                     SAVICINDPLRKEAKYVISNLRECGIKKIVMMTGDSEKTAKSIASKVGVDEYYSEVLP
                     EDKANFVKSEKLKGRKVIMIGDGINDSPAISESDVGIAMSEGAEIAREISDITISADN
                     LNNLIVLKQISNKLMKRVDLSSKFIIGFNLGLILLGVGGFVRPSTSAFLHNASTVGIS
                     LNSMTNLLENTNTYNYH"
     gene            complement(20229..25439)
                     /locus_tag="KPD58_000732"
     CDS             complement(20229..25439)
                     /locus_tag="KPD58_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HBG0264516.1"
                     /translation="MANKLYSEIVNLLEEGRDELRKYDLKEKSILLFLGASGVGKSTC
                     INYLKGCVMEEKTDEETGQIYITAKDSAVEIGNGVYSKTLCPEVVDIANRDFSLCDCP
                     GFFDNRGAEYMIAGAMLVRETISTSSKVKGMVVILDYQSLLDSRHTLLIETGKNLKDM
                     LGDYAKYKENFFFLINKIPKALIGNVTEEKIRNILKKAKEDLDSQEKDYDGYKELVYL
                     LNVALSDGVSIKLCNPLDKQPEKLLNEMKNLPETFEKTDLGLMLTASCKIEIQKAVDV
                     TYKDLEKLLVPYIESVKKGMEDSISILSSINSLKDKKIQLDKMYTDLCSRTNNMKELM
                     EFVQELSYGDGVQKVNILEETRLLLELNSYCSETHIVGLSELLKSLLKNTLEDTLKDI
                     KYWIAEEEYREEEAKKLEDAEYRIEKIQSCTYIKSYEKENVLSIYIPIPKVNLSDLTK
                     IISGYQLNILSSIYMYGDCELKIDCNMEKEVFSGKNIVLLTEKLHIPEGTYSINVSGK
                     DAEKLVGESSIYDGMNGRNGNNAGTSGDIILSFKEIEKRGELKLYVDGGRGGNGQDGK
                     KGDTGITGEAGANAKIGSKKESKEFANVKGTKYYVVKAEKGKQGGTGGTGGNGGNAGL
                     GSKSGQVYLLGKAPLLKSAISKKDGNHGLAGKGGSGGAGGAGGLGGIRGIYEETIRYI
                     GTSPPMDNSDRNYYYFDRYVFNHALNQKQAADDGEKLNTLYEEHKDWTISKREDSGSA
                     GAEGKAGHSGDALKEKELLKQEYSLSIDTFFYEFFERFGFSGIKGYRNLRLLCAVGIL
                     EADILELSHIYEIFHAVEKIEMQLNREILQKYYSFEKKNKAVLSLDDMNTKKEFYKEA
                     KEIYEYLINKLMQSDRIESFVGKNQNFSEWSMILFALEQKTAFYVQMIEESEIGGNRV
                     TDIRSLTDYLDKTLRELEQGKKDELKEGYFNTYKESILTQIESGNQQIEVLKNELENK
                     MLSVNAKMNLLIQEIIKMKEDEQEDIEELKKKQAKLEEQMKTQLILSGIQSALSLLTA
                     FSGVGQACLGAFDSAKSLKENLSSKEEEYKWTNRMVNFSDFKLDSDGMRFVLGEQLSE
                     DRKNALDKMTYNLAGVDEERRTKFDQIKEIIVKSIDDDNYADKADDIQRAKEGLSESE
                     YKEIYTKIQKKEIGMLEANYNNKQQKDKLAALEKSKQAIERQQKTSKIKNALSIGSSA
                     CSLINTAISGVNNCLNVKSSFESKIEVVKEAIKDKENNIKKLDELNTQMVEFKDKTLK
                     ESVIPFVEKFDSTVKNQDIFENEISKLKMKEVLTELSTKFGSLKMENSEEISIIFKKV
                     QDMMETQIIITDKIDKKKDNLAMGELIYQMSDSGNQMNEYQKKLIHQIELNQVRYLCQ
                     VEAAAFQLWTFPFGSNIVNILNRCHVGITEDENEVINQAKEQNRQIREFLDQDAYQWQ
                     KTDNHILKVALTGSLDGKSRVGFENTYSLYDCNEEEVKKLLTGDEISFEVPLNKNFDA
                     IKYVTMYANIPNLRRNILEDSLKVKVHIHLYDSGYFSLYDDKKDVYYQYVFPQNYINI
                     THGLDVKYNDSDSINSIWSSVEDKGIEKFKNAGLNSGRSPYSMAKISLKAHFNRKLSY
                     DSLTNEQKKKLESYFDKPSKYAWSDSIDILRSLGYIDLKGILSKSWYEIKKDIDSDGE
                     IMKHFSILQVDLLERIIYEQTSILKENIPNLTIELVGCGIYVESEFNYLKQNDLKCYE
                     ERLVENTIIG"
     gene            complement(25454..27949)
                     /locus_tag="KPD58_000733"
     CDS             complement(25454..27949)
                     /locus_tag="KPD58_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0264517.1"
                     /translation="MPENNNQLLEEITLLLNEGKVAINKAENQNLILILGGTGCGKST
                     AINYLAGCVMMEVEDEDTGISYIDCLDPVAEIGSGAVSQTLYPNIVNMRKPIEGMDAK
                     LCDTAGFSDNRGVAHDISAAVSLGEVFKNSASICAVIVLIPEADILDSRLTKVLDLFQ
                     QLNMCLHKENFKGSITFFISKSFLGRTEKQIYNVIKRKYEQLCENLDSNIEEKAWLFE
                     ELLKDNGKNIHLCKPLASDERDGLLKQVLELDEVKDKTSAFQYPISADARLVLDTLIK
                     EIQNNIVSSLEDYVKAYTEDFISIIGSITMIQEIAKYDEVVKVFKNWLESTTSLTIDR
                     FINVLAKQSGLFLQDSCMKLQNKIKGNLDIIDALSKYTEHGTGEILLPSVHMEFIIEK
                     ASKKIMEQNFIIIRKNLEKVLVSYEVQKQVFTLPNREKLMEVANKKDYVYSVSDFNSI
                     LTNVTVTQNEELEDSITRLSAYGEEGNEIIREFIGKYILNPFIVKTININDKKGIEIR
                     ALIPNLVISSVLEYFKNRKVDDNVSTFMFFAKDTIYMDDNLGMDFASEKNIILACNNL
                     DVGSNVKINVSGKSGELVDSSMYGKNGEDGRDSGNIYLYIKEEIRNYRLTLIANGGNG
                     SKGIDGKNGETGASGSNGADAGYTSYFHGQMFKGYFAKGTSGKKGEKGGAGGNAGRGG
                     NAGKKGNIYFISDSEEQKNRIINHCDITVKDGEAGPNGRPGSGGYGGSGGYHGYNTLS
                     FSPSGIDKTVYYTGYFTSWDYKLDSIIKYHIEFSGGSWNEGKTGPAREYAEYGDRGDN
                     GGFNAPNTKLSTAVSKVEENLIKEKYQGYCSEI"
     gene            complement(28586..28873)
                     /locus_tag="KPD58_000734"
     CDS             complement(28586..28873)
                     /locus_tag="KPD58_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264518.1"
                     /translation="MRLNVTKQNTIEVENSRKEDVYFVIVDNVFFERQGDVFNSITNN
                     NLNNYDVKILPTDNVFLELKITSTKDLEPVFIVVEDRQYNHGSIMFKPVLV"
     gene            complement(28893..29168)
                     /locus_tag="KPD58_000735"
     CDS             complement(28893..29168)
                     /locus_tag="KPD58_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264519.1"
                     /translation="MSLFVGGWSKFRAVEEEDLKVFNEAVGMLEGVGYEPLIVSTQVV
                     AGTNFKFICNATSVTNPPQNYLAEIVVFKPLPCDEHNATITSINIIK"
     gene            complement(29801..29980)
                     /locus_tag="KPD58_000736"
     CDS             complement(29801..29980)
                     /locus_tag="KPD58_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264520.1"
                     /translation="MGKDRFEKVYSQGKINATEIWVDKETGVNYVFHYYGNAAGFTPL
                     LDREGKPVISPIVSK"
     gene            complement(30265..31731)
                     /locus_tag="KPD58_000737"
     CDS             complement(30265..31731)
                     /locus_tag="KPD58_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0264521.1"
                     /translation="MKRKPQYLIIFEHIIQQIYTGKITIGDRLDSIKSMSEEYMVSKN
                     TIKTVIKMLSEKGFIETKAGSRPVLIQSTSKQSIESDLLYEKILQISEIYKIFSLIFP
                     SIAMYNIKRFTSKDFEELNEIIDCMEKNSSNYLVFKEQRLKYINKLISKTNNFLIHHF
                     CEIIQHDVIISQLAYNFDNLDNCCIDFFRNKYIEQVRKTYLYGLDGNLIEFKKLLTYM
                     YKACRESILKIVENRAPQSIDTSSFNSVTLEKCYLYDLIVSDVICQIFEGNIKIGDCL
                     PSITSIINRYNVSLPTVRSAYNELNELGIAKTINGKGTYVTLFSEYDDDYINTEEGAK
                     RLSLLLDAMEFVTITLEDVVLLLGNKMDLIAVDKIETHLRYLQNNCEECKVYPDFVLL
                     CKIVDYTQIYVLQETFLHLKQYMIFGIYLERFFHEEYNEILKTRFDVCFEILRYLKKG
                     DVERFAESFSNIFKESLQYLKKVISSLNVDMRVKLDIL"
BASE COUNT        10885 a         5522 c         3636 g        12017 t
ORIGIN      
        1 tttttgaagc ttagttttta gagttttttt caaatttaat tttttgaaac ttaattttta
       61 aaatttcaat cttttaaaac tcaatttttt gaagcttaat ttctagaatt ttatttttca
      121 aaatcaattc tttgaaatct aatttccaga attttaattt ttaagattta attttttaaa
      181 acttagtttc cagaatctta attaatagaa aggtatatta aatgggcact tattctttaa
      241 tctctacaag cataaatttt attctaatat taatcaatat tttcatagcc ttttttcttt
      301 atacgttgac ttcctatttt atagacttta ggaatcattg gattttcaaa atcatattga
      361 ttttgggcta ctctattatt agtcaaatga taatatggct ttccgacccc gtaaatattt
      421 tgtttacact attcggatat tttatactac ttataatatg tagcaaaagc aaattaattc
      481 ctataatatc agttgtcatg atactttatc ctattataat tgggcttaat tttcttttta
      541 taaacaaccc tatatatgag gaacttatac actcaaaaga gtatatttta atactttcaa
      601 tagtccagat aatttctgct ctaattaaat tattattttg gtttttaata catattttcc
      661 taaagaatag attggtcaat ataaaacaat acttatcaaa taaaatatgg atttttattg
      721 atatggtatg tatatcttca tttttatcaa tattaattgc tattattctg ccacctacta
      781 atttaatccc tatgggtagt aataagaata ttatcttagg caacttacct tatggcactt
      841 atctaatcat cttgtcagca attctggcaa atatcggtgt gatacttctt ttacagccat
      901 tgattgaaaa tgtaaagatg aagatagaaa aacaagcaaa tcacctaaaa gaaaaatact
      961 ataacttact agaaaatcaa caaattcaaa tacgcaaaat gcgacatgat atgaataatc
     1021 actttcaaat gattgaatcg tatttagaaa ttgaagatat tgctggagct aaaaagtatt
     1081 ttaatcagtt aaaaataggt ataggtactc ttagtggcaa acaattttgc aaaaaccaag
     1141 ctcttaactc aattttaaat cttcgtcata caagtttgga agaaaatggt gttgatgtgc
     1201 actttaatat agatatagac aatcacttgg gtatagaacc tatagattta tgtactattt
     1261 tttcaaattc tttagataac gctatagaag catctttgaa aattcctgat ttgtcaaata
     1321 gaaaagtggt attaaaggca cgttgtgaga aggagtattt tagctattta ttgacaaaca
     1381 acaaagtaaa tcctatcaat agaaaacaag aactatttat ttcagataaa aaagatagta
     1441 attctcacgg atatggtatt gagaatatta gagaaattgt taataagtac aatggaaaat
     1501 tggatatttc ttatactgag agtgaatttt ctttatttct ttatattaga ttattttagt
     1561 ctaaaattag attatcttat ataatacaaa ttagattgtt tgatactatg taaaattata
     1621 ataacttata attaaatatt tacacatata ttcattaact taaaatttaa tcaaatgtga
     1681 tattttattt accttagtaa aaatgtgtgt gtctcaaaat aaacttttta gttgatgagg
     1741 cactcttttt tacatgaaat caaatatatt tttatccttt caacaatgaa aaagggattt
     1801 atctctccat tttgaaacaa gccctttttc tattaattac tttcttaaaa aatataatac
     1861 gatatataag ataaatatct aattgtacct atttctaatt atatctatta gaatacaaca
     1921 cctttgcctt ttcagtaaac tccctaacaa aatctagttt actatctgta taagcatcac
     1981 gattatgctc atacttcttc caaaggtcca acttaagttt ttcatactcc tttgcaacat
     2041 ctgggtggtc aattaagtaa tctctaaaaa acaactcatc attatcacct acatacctta
     2101 tatgcaaatg aaaaactcgt tcttcaaacc catctactgt atacccttta ttaagtgaaa
     2161 gcctactact gctctcagac atacaaatat atccattcga ctcaatcaaa tctttaatat
     2221 ctttcatatt ataatcagga attatctcaa tcagtatgtc aacaatgggc ttggcataaa
     2281 tcgtactcac agaagtactt ccaatatgac ttattctaac aattttttgc attggcaata
     2341 ttccagttag tctatattgt tcttctttaa accaattttt ccaacattct ttgtgagtag
     2401 tcaaaaatat aggaaacaag ttccataact cctctaaagt catatcttcc aacttctttt
     2461 tcattagtta cctcccttta aatttgtact ttatattttt tctttttcaa atttacttag
     2521 ttaatattta actttatttt ttatcaaatc atttttctaa ttatgtaaat ttttctaaat
     2581 ttagttttta atgtattata acagattttt acccttctta cacttttgta tctatttaaa
     2641 tattagaaca ttttttctta cttctaaata cagtaagatt gcttaaatac caaataggag
     2701 caaaaaaact ctgtaatctt aaaaaaacta caacatcttt atgagaatta aaaaatgata
     2761 gactatgtta tcaacattaa gaaacagata gaaaagaaac agagtatgtg ctaaaatggg
     2821 aacatagaac agaatgacaa attggaattt atggaggaca ttaaatatgg agtatataat
     2881 caatccttgt agtggtataa aaattaaaat aactagaggg cagaaaatta aaattgtgga
     2941 tattgaagga ggacaggtcg ttgatttttt tgcagaaatg aaagatgatt caactgaatt
     3001 tatatctcca ggtgttacaa ttgattgtaa tgagtcatta agattacaag ttgggaattt
     3061 aatatattct aataagtata gacctatgtt taaaatttta tatgatgatg taagggaaca
     3121 tgacttgcta catccatgtt gtagacctga gatgtacgat ttcttttatc acaatggaga
     3181 aaatcatcca aactgttttg ataatattaa tcgcacactt ggaacttccc atcaaattat
     3241 tcaccccatt aattttttta tgtacacaaa agtcaataca gatgggagta ttgaagtatg
     3301 tgaaccttta tcaaaagcca gtgacaaaat tattcttgaa gcattggaaa acatgacttt
     3361 agggattgct gcatgcagtg tttcagaaag ttcttgtaat agcagaaaat gtacttctat
     3421 taagattttg gttgattgac aaatttcatt tatcgaaaaa atatcaaaat taaaaaatga
     3481 cgtcaaaggc gcaaaagggt aaccacttgc gctctcaatt cattatggtt tgttacgtat
     3541 ttatgggtaa aaatcccttg attctatgat gtttttcaga cacaattaca aatgagtaag
     3601 gattttatac tcatactcta aaaaatagca ttttattgcg taacagaacg aaaaaacagt
     3661 gtgtaccttt ttcttgcaat acacactgta ttcatagcca ctctagctgt taactgttaa
     3721 ttatctcata atacccttac tggtattcat ctaatcttaa ccccttttaa ttattttcat
     3781 ttataaataa attctattct tcccaaaata tcgtaattaa aatatattaa ttaaaatatc
     3841 ccaataagaa tatctgaaaa atcttctacc tattttttca aaccttttat tcccattcct
     3901 catacaatag tgcaactttt agcacagtat aaatatacca ttagtccata taatctcatt
     3961 aacaaatata tacaattatt ttcttcaaac tcttttatat ctaagatatc acattttcat
     4021 tttcttatat taattcctta actctctttc aaattaccaa ttttttaatc aaatcttcat
     4081 taaatattct atcattatta aacattcatc attatcccta aaaaatatgt gccgtatgtt
     4141 ctttacacaa attttccatc ttatcataca cagcaccact atctctctca tctgtcatag
     4201 tcacaattac atcacttggt ctaagaattg tcttacctct aggaataatt tcttctccac
     4261 ctctttgaat tgctaccaaa agacaatttt caggccactg tacatcctga attgccttgt
     4321 tcgcaacagg agagccaaac ataacagaga actggtctag tatcttttcg cccctctctt
     4381 tatgtacagg ttctccattc ttttcaagta acctctcaag aagactttca taaataggtt
     4441 tagacttcat aagtgtagcc acaatatatg ccacaattga aattactgat agtgaaagca
     4501 tttgactaac agaacctgtc atttcaaaaa ttaatatgat accagtcaaa ggggcacgca
     4561 caatagctgt aaaatatcct gccatagcca aaagcacaaa gttatttaca taatcgggat
     4621 tcattccaaa aactttaaca cctaccatac caaatattcc acctataaat gctcctaaaa
     4681 caagcaatgg aaaaaatatt ccccctggtg cacctgaccc aaaactaaca gccgaaaata
     4741 taaaacgtat aacaaaaatc aaaacaacca ttccaaaaat atatttatga cttgtaagtt
     4801 cttcaactaa attgtgtcca ctccccagaa catcaggcat aacaagccca agaataccag
     4861 ccattataaa tggaatcaac agttttccaa acgtaccaat tttaggaatc ttcttgtata
     4921 aagactgcac atatagagta aaccagttgt aaaatgcccc cataactcca agaataacac
     4981 ctaaaatcac tattaatcca taataatctt gtggcaacac atttccaatc tgaacctccc
     5041 atgactaaag tcacagggtt cctgcttcat agaattttgc atactaaaat atgtcttact
     5101 aattctccac aggctatccc cgtagttcct acggttctta cataagttat ttattttgaa
     5161 ttgttattag tcttaatcct tcttttaata tatttatact tgcatttata tctctatcgt
     5221 gagtttcatt acaactagga caaatccatt ctcttataat aatgttaaca aatgtaatta
     5281 tttaaaacaa ttatttagaa ggttttatat ttaattttaa ataacatatt taagaaccgt
     5341 aagtcttaac ttttatcatt tttgaggttg tcgttcacat aagttcgcta ctacctatgc
     5401 agttctctta tgaacttctt acactttcat gtaagcacag actatatctt atccttcggc
     5461 attatccgct aaggtctacc cacttccact accaatagct tgtagtgtac ttccctcaag
     5521 aggaatagtc gttgaagttt ctcctattcg gagcttacct gctgattgcc catttttaaa
     5581 gtacttaggg tttaacctta taccatctaa ccaatttttt ctactttcgt aactttcaca
     5641 cttaggttta tttcatcctt atgttttagt ttggttagct ttaggggttt ccagcaattc
     5701 aagtagtatt ggataggttc tatcctatct ctacatatag gtttccctat atgctgacta
     5761 ttttagctac taactcatga ctaaagtcac aagtgttcgt agctattttt taatcaaact
     5821 ggaatacaga ctccattcct aggaaattgg ctgaaatata gtctgctgtc aaagatgctg
     5881 tcatcacaga aattaaaaca gatactgaaa agtttttatg gacttcttca agtgaaaaca
     5941 taacacccgc tagtggtgca tgaaaggctg ctgataatcc agcacttgcc ccacaggtta
     6001 acaggaattt ttcctctgtc ttccctcggt caagagctct agaaatacct ttacctgcca
     6061 tagcaccaag ctgaatggaa ggaccttccc ttcccaaagc aagaccaccc atgagtgata
     6121 agaatcctcc aataaatttt gttggcaaca ctcgccacca tacttgtttt attttccctg
     6181 tcatttcccc ctcaagctga gggataccac ttccagaaat cattggctcc cagtccacca
     6241 actttgatac aatccacgca agtactgcaa gtactgcaaa ccaagctatt atttttattg
     6301 tagaaccttc tacaaatttt aaaatttggc taagccaagt accagcctgc cctaaaagta
     6361 tcctgtataa aagcacaatc aaaccagcca cagccccaac cataagtcct tcaccaatca
     6421 aaattacctg aaagtgctct gcccgtttga taatatgaga tgtatccttt ttcatactgt
     6481 tcctccttca tccaataatt ctacacaatc tgaatctcca atgactaaag tcatgagtgt
     6541 gtacagatat tttttaatca acaaaaatag atttttattt actaaatata agaaaataat
     6601 tgctcaattt ctcaataaat gtgaatttat tcttttaggt atttattctc cacagtcgtg
     6661 cttcttccag caaaatattt attagttctt gctttctctg tgtatagatt ttacgattgt
     6721 cctcaaactg taatgctaat ttttgtttat gactatcata gagttttgct ttgtctgtaa
     6781 aagtatttag ataatctcga aaattgatat aattattcca tgcaacacta ttccattcaa
     6841 caacatgaat atggtgagta cgagtatttt tctcaaaatc tcccatagca aaaagtaatt
     6901 gttttggtac atcctgacct cgaaaaacga ttccacattg ctctaacctt tcgaggtata
     6961 atttagcttc ctctaaagaa gcaacaccta caacaatatc aataataggc tttgcgctaa
     7021 tactgggaat agaagtgctt ccaatgtgtt gaatatcaat ggcagtctta tcccatatac
     7081 ttttcaataa tataatgaca tcttttgcac ttttatccca tatcatctga tgttccaata
     7141 atttaaccat accacatttt aaacctatca ctatatacca cctcaatata atccaccttt
     7201 atatatatta caattttttc tatcttgata aacagtgatt tagctatttc actttttctt
     7261 gacactcgct acattcccta tcatgaatag atataattcc tccacattta gaacaagtat
     7321 atctctcctt ctgcttttgc ataaacttct ctaaaccgta ctgctgaaca aatttactat
     7381 tttccataag gcttgcttga tatctcttgt tataactttt ttcaagattt tttatgagtt
     7441 tacaaggaaa attgaaacat tcaaaacaat aaaacaaccc tttttgacag acacaatcct
     7501 ttattttaca cttacggcag tgttctggct ttcctgtatc gccattcaaa caaccagcac
     7561 atggcttttt gtgataacaa tgcttatagc aaaccataca attcattcca caaggagcaa
     7621 acataatcgt atcaatactc tcttttggca ttttcataac attttactcc tttcaaaaat
     7681 ccaatttgtc gaattgttat atattcatat tgtaacataa aaaatagcta aaaaattcat
     7741 gcactttcat tgccaataaa ttttcaagga aatatgaaag tttaaaacta agaataaaaa
     7801 gagataaaaa ggaagctact aatcaaaata ttcaaataca agtaatcaat ataaataact
     7861 taaggtatat ttaaaagtat atgatattac cagcagtttt aaatacaaat ataaccacac
     7921 atgcctcaaa aacacttatt acagtatctt agaaacttag acaatacata agattcatta
     7981 ataaaaaagt tctcaatata gtcattaata aaaacgttct caatatagat atatttaacc
     8041 atgttttgaa tagcagataa ttatatgatt aaaaattaaa tatgacatag cgtttgtgga
     8101 atataacacc taaattatgt tataataata aaacaggagt gtgatagtga tggatgaaaa
     8161 aatattagag ttattacagg aaatgcaagg taatattagt aatatgcaag gtagtatcag
     8221 tggtatcgaa ggcactatta gcgatatgca aagcaatatc aatggtatcg aaggcactat
     8281 tagcgatatg caaagcaata tcaatggtat cgaaggcact attagtagta tgcaaggcaa
     8341 tatcaatggt atcgaaaaca ctattagtgg catgcaaggc actattggcg atatgcaagg
     8401 tagtatcagt ggtatgaatg gcactattag cgatatgcaa ggcagtatca gtagtatgga
     8461 tggcactatt aatagtatgc aaggcagtat tactgatatg caagttgaaa ttaaagaagt
     8521 caatagtagg ttagacaata tggaacctga tattaaagac ttaaaaactg gacaagttga
     8581 aactagtagt agacttgata ctattcagag tgatgttaca gaaattaagt cagatgtaaa
     8641 aattaacaga aatacattag atacagtgat taaacatacc tcttcattag taaaagatac
     8701 atcagatatt aaaaatgatt taaataatat agagatagtc acatctaaga attcgaatga
     8761 tattgctaaa ttaaaatcag ttaaataata tactatagta aaaaatacct gaaaatttca
     8821 catacttctt taaagttata ccaaatttta tacagaaata ctgattatcc gacaacacac
     8881 tttacttata aataatttat taaaaaaata ctcattttat cctaaaatta acacgactaa
     8941 atgagtcttg atataataat aaaaaataag ggagattgga cactaaaaag ttgtgtatct
     9001 cccttatatt ctataatata gttatataat ttttcagcct aaaataaaag tccatctttg
     9061 attaaaacac atccccttat cacttataaa aggataaaat cttgttttca tttttccatt
     9121 tatatcttct tgcttaagag taataatttt ttcttttgat actttaagtg tttggttaaa
     9181 tttaaccaat cgactaagtt ttgttcaata tctatttttc atattttcca atacttaatc
     9241 tattttttat aaataattct ttcatttcta actcacttac attttttaca taaaacgaaa
     9301 catataaatt tcaaccttat cttgtatggt tcttaatact taatgagtag cttaagtatc
     9361 aatcattata aatttttccc ttacatttaa cactcttacc atttccaaaa tatacaaaat
     9421 atattttgaa catttgtaca atgatgaaat acaatacatg aaaatacaat atcataactc
     9481 ttgtctaaaa atagaagttc ttcaacataa cctttcacaa aaaacatatt atctaagtat
     9541 tttttgtctg ctccttgttt tcctgtttgt agcatataaa ttactgcatc taaacatata
     9601 actactcaca cgaaaaaaga aaataaactt ccaaaataca agctctaaaa actacctcta
     9661 aagttgtata atgtttacta ggaataaggt tgtataacat atagtttaaa taatcatttt
     9721 ttaaaattgc aagttattgc tttcaaaatt agatgttttt atatcaaata aatgattaag
     9781 aatttctaaa tttaatggct ttataatgca ctccctttct ttgggataaa ataatgttgc
     9841 acagatactt tttctaaaga taacaatttt atttttctat caattccacc agcataccca
     9901 gtcaaactcc catttgttcc tacaacacga tgacatggaa caataataga gattccatta
     9961 tgccctactg caccaccgat tgcttgtgct gacattttag atattccttt ttgttgagca
    10021 actttatatg caatttctcc ataagtgatt gttttaccat atggtatttg ttgtaaaatt
    10081 ttccacactg tagtctgaaa aggagtacct accatatgga gtgatggtgt aaaattgggt
    10141 tcttttcccg aaaaataaat atctaaccat ttctttgtct gctcgaaaat tggcaattct
    10201 ttttcttgat gttcttggtc aagattataa gcataatact tttgattctc aaaccaaaga
    10261 ccagttaacc caatattatc tgatgctaaa agaatatttc caattggact atggtaatag
    10321 ctaatatatt gcatacaatt cctccttata ttctgaaaac ttcactcaaa tcattagaca
    10381 cttcttttct atctaccatt atgtaaaaac atactaatat aatttccttc taactacact
    10441 ttcgttataa tatctatcct catatcatat tagttttata tttacctgca tctgttcatc
    10501 cttttttccc tttccctttg atgaatataa aattcataca tttaagcttc ctactgtatt
    10561 aacttttata gaagtgtata ttataatttt atcaccaaat agaccaaaaa aatatcgata
    10621 taacatcaag taatatcata atactttcaa atgggaaaag taagaatagg ctgtaaaaaa
    10681 gaaaagtatt tttatgttag aaatccagta aaatcgttag tagatagaat aacagaaaac
    10741 gaacaatttg ctatttatca ttttacacat aaatataatt ggcgcaataa agtgttttct
    10801 attctacaac gattaataag gttagaaaaa agtaaaaatg aactgtatat gtatgaagca
    10861 ttagtttctg tttctttcct ttggcttatt atgataaaaa atattgtttt gccaccaata
    10921 aaacacaaaa atataattaa tatccatatg caaaaaattt tacgttatat tgaaaaatac
    10981 tattgtttta cattatattg aagggtacta ttgtgaaaac ttaattttgg agtactatca
    11041 agcagtggta atatcaataa atcagagtgc tccagatgat ttgaactaag tttaaatact
    11101 ataccttata aatattttac tgagttttgt ttattaaaag caactcaatt tctaaaaggc
    11161 acaaatgagc taataagtga cattgctata aaagttgttt ttcatcaaat aatctatttt
    11221 agtaaatgtt ttaaagaaaa aacgggatat tctccaaaag tgtacagaaa tataaaaaaa
    11281 gagatatgat tttttatttc ttttgattat ccaaagagta aataaaaaat attgtaaaaa
    11341 acttactaac aacggcaatg ctttgaacta tggtaatact ttgaactttt gattggactt
    11401 tttttgctaa aataatatat ttaaatagca aagtaatctg tttaatcaat aaaaaataag
    11461 agataaaaga aatcaactaa aacttttacc tcttattttg atagtttatc agagctaata
    11521 tttacactct ttttattttt gatacttcat taaaaatgat gcttattctc ttttcatttt
    11581 taatacttca ttagaactta tgcttattct cttttcactt ttaattcttc attagaactt
    11641 atgcttactc tccattcact tttaattctt cattaggact atgcttattc tccattcact
    11701 tttaattctt cattaaaaat gatgcttatt ctccattcac ttttaattct tcattaaaaa
    11761 tgatgcttat tctccattca cttttgattc ttcattaaaa atgatgctta ttctctgttc
    11821 acttttaatt ctttattaaa actgatgctt attctccatt cactttcaat tcttcattaa
    11881 aactgatgct tgcactctgt aaactcataa acatccatta atgcttcacc aaatctttga
    11941 tagtgaacta cttccctctc acccaaaaat ctcaatatat ctttcaaatc agggtcatca
    12001 gttaaattta ttaattgata gtacgtagcc aatgcttttt gttcagcagc catatcctca
    12061 tgtaaatcag ttaccgaatc gccaaagaca ttaatataac ttgtagtaaa gtttactcca
    12121 ttagaatcta ctggctgaag accatgtcca aaattagcat aattactacc aagtccagca
    12181 gccttaagtt cttctggagt acaattgcct attaattgat ataacactga agaaattatc
    12241 tcaacatgag ccatttcctc tgtacctata tcagttaaaa gtgcacgaga ttttccagtt
    12301 ggcatagtat atctttgttg aagatatcta gttgcagctg ccaactcacc attaggtcct
    12361 cccaactgag tcatgatata ttttgccatt ctagggtctg cttgtcttat gttaactgga
    12421 tgttccagtg ttttttgata aatccacata ttatcttact cctccttaat atatatttaa
    12481 taataaatct aattttaaag tttaataatt ttatttataa aatgatttgt cccatggcca
    12541 aggttcagat atccatgacc aaggatattc acttgattca tatccaaaat tagttaatgc
    12601 tccatattta gattcataat cacacacagc ttgtgtaaat ttatcgcagt atctgttgta
    12661 tgaatcaaga gctttcttat cccttggatt gttatctaaa tataaattta attctataca
    12721 ggcaaattga tattctgaaa ttctctctaa aagttctctt ctatttaatg acattagtac
    12781 atgttacccc cttttttagc tctaactcta tttttaggat ttgcatatag ccaatcatta
    12841 tagttatttg actctattaa atctaattca ggaaataaag tacctctttt taatgctgtt
    12901 tttggattgt atgtgtcatc ataaatttga cgattgatgt atggtctagc aaattcacaa
    12961 caatagtctt gtttatcctc ttttaaatat cccattaatt atcctcctta atttttagaa
    13021 ttttaaaata gcattatttt taaaaattgt atactggttc tatattataa ctggtagttt
    13081 taataggtct ttaataagat taaatgatta aataagtatc tttttttctt attttacctc
    13141 taaaaatttt tctaattagt ctttcataaa tatctatttt aaataaataa tatagagttt
    13201 atacaatttt ttatagctta attactattt actatatatt attcattttt aaataaaatg
    13261 tgattatttt ttactaaaaa cgatataaat atacaattta ataacatttt taacaaataa
    13321 atttatattg aaatacatta aattatataa ttttatttat tctgagagta taattataaa
    13381 aaaaagaata ggatattttt cctactcttt tttctttact tatatataat attaaaactc
    13441 tttatttttt aatatatatt tttcttttaa tatatctctt ttatatctct ttaattttct
    13501 tcttctatat cagagccaaa gggaatccat gcattaccaa actctttatc tttaaataaa
    13561 taagctaatc cagttatttg cttcaatcta agaatttcat caacttccat acccaggtgc
    13621 tttgatatcc attcatcaga ttttccaatt tcacaaagtt cagttataat tttactcatt
    13681 aaatccacat tatgagaacc tcttgcacga ttgtgtcgaa tagtacttgc cattctttca
    13741 tcaagagatt tatcaattac tgaaacagga agaagtccgt tttctctatc atatatatcc
    13801 tcattttcaa gcattactct atatctatga aaaccatcta caataatata cttatccttt
    13861 tcctcattat aataacagac aattggcatt gtatacccat cttcttttat acttttatac
    13921 aacaactgca tttctggtgg ggctactgca tttggattat agtcgttaga ttctatcttt
    13981 tcgattggca ctgcgatgat tccatataca ggacttttat attccatagc atcttaactc
    14041 ctatctaatg aattttttca tatttcttct taatatattc cgttctaatt ctctgttctt
    14101 ttgtaggacc aaatcccata aatctacaca tatggtcatt ttttaaaata caataacaca
    14161 ttcttttcca acttggtata tctttggtaa atttaatatc atccgtatca tcaggtatat
    14221 catccaaaaa tatgactcta ctctttttat cttttgtatg atttgaaact ccatttaatt
    14281 ctattttata accttttctc aacaattcac gtataactat ttctgacaaa ccaccacctg
    14341 tattctgcca aaactcaata gatgttgaaa attttttaat atagctattc cttatatttt
    14401 caggtaaggt atcaagcaaa aaatatgtga aacttttcca tgtgtgtcca tatggcaatt
    14461 taacactacg atatcccata gcttttgttt tgccataaat agaagtaaaa ttaactcctt
    14521 gtactctagc aaccaatttc acccatacat ctgggtccaa tattctgtat aaatttaaac
    14581 tctcttttgc tgaatcatta aaaggtgatg ccactctcat ttttcttgga gctactccag
    14641 ctttatagaa taagtcataa attttattat agtcatagtc aaatttatag tgtgctaacc
    14701 atatatcttc tatttcccaa tcatatatag gagatgccgt ccagacattt ttaaattcct
    14761 tagttatcca gcactttcct ttatatccat gctgtttatt cacaatagaa caataacgtt
    14821 gtagagattc tgctgctcgt gttcctaata agcaaacaac ttttgcacct tcatgtatat
    14881 ctctaatcca tctaccaaat cgttttgcca aactttcttg tctcatttta tatttataat
    14941 aagtaaaagg attatttttt tgatttatta tgtattcttt gactggcatt tcccttaccc
    15001 ataactcttc tttctcatca tcccatggat accaatacat ttcataattt cctacagcag
    15061 ttcttgtagc cattggtaaa cacacccaat atggttcaat atcttcaata ttatcttcaa
    15121 atactttttc aacatattca gtagtcaaac tatattgagc ctcaaaatct tgatgaaata
    15181 ctcctatttt ggaacttata ttattctttc tcttataatc caaaactaaa tttagcagta
    15241 aaccactatc ttttcctccg ctaaaagata catagatata atcaaattca ttaaaaagat
    15301 aagaaagtct ttcttgaaaa gcttcaaata catcggattc taaatatctc ttatccattt
    15361 gaattatccc ctttcaatta gaagtttata aaaccttaca atgcaatatt attatgcttt
    15421 tttagcagta atcagcataa attatacatt atttaaatac acaaatccaa ctaaatacat
    15481 ataattataa tgtaattata cttttgtatt ataaactata ttttatcatt attttacatt
    15541 tttttccaat atatataatt atcatatttt tctattagct aaaactaatt accttatatt
    15601 tttacgtatt ggtataataa tacataaaaa cttgaataat gcataaaaac ttgacatact
    15661 gttgtcataa ttactttgct ataattaaaa gtaaaaatgg aggtaaattc taatgtatga
    15721 acgtatgctt gataaaagca ataaaccctg tttgtctcaa ataatagaat atataggtaa
    15781 agatggatat gcacgattgt gtaagttgga aacttacttg aaaactcaat ataatttatc
    15841 aagagaactt cgatttcctt ttggaaacaa ttatggttgg ggttacaaat atagccataa
    15901 atcttttcat ttattttatg ctttttttga aaaaggtgca tttactgttc tgattcagat
    15961 aggtgacaag caagttccag cagtagaaaa aacgttggta gctttgacac caaaagctaa
    16021 agaactctgg gtcactcgtt atccttgtgg caataatgga ggctggatta agtatcgtat
    16081 tttagaagat gcagacttac ttgatattat tcaatatata agagcaaaaa aatctccacc
    16141 taaacaaaat taacttttat tttttgtgat ttaaatatag tgtatataaa ttccattctt
    16201 tccatataaa aaaaataata taaaaaatat ctattagtat aacttattta gtatgtctta
    16261 ttctactttt tacagataat taaaaagttt tatcataaat aaatcataac tagacatcct
    16321 agtaaatcaa aattaattta ttctttttta ttagaatttt tgataacagt ccctatacat
    16381 atgccaatta tcattccaaa aattagacca taacacattg tgatacctac atttccttta
    16441 gaaataccta tagcacaacc tatagccatt ccaagtatca tgcctataaa catacatata
    16501 ataactaaat tgttgttttt attcatagtt tttctccatt caatatattt taaattgcct
    16561 agctcgcctt cttagcttaa aacaaagaaa tataaattaa ctttctttca ttctaatatc
    16621 tgcttgtata aatgtcaaac aacacatttc ttgtcataac ctcctctttt tgaagtaagt
    16681 acaatctccc agacgcaata atatcaattt cctaaaacca tttaagtaac ttttatattt
    16741 acataatatt tacaatcata aactatattg ttattcattt atacatcata aacactccct
    16801 cctaatcatt attatactta aaataatgtt catttataat aatatatcta acgcacttta
    16861 attgaaaaaa ctaaaacttt gcctttcatt cccatatata aataagtata ctttttgatg
    16921 ctctattaca aatcaactaa attaattgat ataaattatc attttctgtt gaaaatacca
    16981 ttcgaacatg ctatacttaa ttgcataaat ggtaataaat gtcatatgat tttacaaatc
    17041 tattaataac aattattact tttactgtat tctaaagttt ttggactaaa tatcaagaac
    17101 tttctaaaat acaaagtaca aaaaatttat ataagttata aatttatatg aactagataa
    17161 gctcaaacaa tatgttatgt ataaactgtg tagtattcgg ttataaattt atataaatta
    17221 tataagctag ataaagtatt acctcaacta tataagctac atatataaat tacatacata
    17281 agctatataa gaattatttc aactatataa actacgcatg taaaattcat gtaaatttac
    17341 taatataaat caaataaaat tactaaactc tctaaactaa ttttgtaaaa catactctat
    17401 ttttaacatt tgttacctta tacataagta caaacaaaat ctaactaaat gaaaggataa
    17461 tgtaaaatga atattttaaa aatgaaaaaa acagggatat ttttaggagg agtacttttt
    17521 ggtactgtag gtgttaagat actttcaagt gattgtgtta aaaaaacttg cactcgtgca
    17581 acagcaggag cattgaaagt taaagattct gttcttgaaa caactacgaa aattcaagaa
    17641 aatgtagaag atattttagc tgaagcaaaa gaaatcaacg agcaagaaga cttatctaca
    17701 tctaaggtaa acgttgtaca agatactctt aaatgcgaac aagaagttct taaaactgaa
    17761 actataaaaa ctgtagaaga tactaattct aatagcatta aatgtgaaga aatcaaagat
    17821 aacgtaattg aagaaaaaaa tagtacttgt acaacagaat aaatttaatg attattatga
    17881 ttaataatat caggaggaag taaattgaat ttttatataa aacacgaaat aagaggtcgt
    17941 atacgctttg acttacaact tggcaaatta aacaacaagc agtccgatac tctcttatat
    18001 tatttaactt ctataaatgg tattaccaaa gcaaaagttt acgatagaac tggtgatgca
    18061 gtcatttatt acactggtga aagagaatac ataataagag aaatcactaa attcagtttt
    18121 aacaatagca aaatagaaaa cctagttcct gaacatacca gcagggaatt aaacaattat
    18181 tatagagaca aattagcaaa taaactgcta gtcaaaagta tctccaaaat attttttcca
    18241 aagtccttaa gatttttttt aattggactt cattctataa aatattttaa aaaaggtctc
    18301 tcttccctgc taaataaaaa aatagaagtt tctgttttag atgccactgc tataggtatt
    18361 tctattttta gaaaagatat caatacggct ggttcagtta tgtttcttct tggtatcggt
    18421 gaactattag aagaatggac tagaaaaaaa tctatagacg acttggctca aagtatgtca
    18481 ctaaacattg aaaaagtatg gttaagaaaa ggtgacactg aaatcttaat tccaatttca
    18541 gagataaagg aaggagattt agtaagtgta actatgggaa atatgattcc attggatgga
    18601 gttatagtat caggagaaac aatggtcaat caggcatcac ttacaggtga atcccttgca
    18661 gttaacaaaa aagaaggctc ttacatatat gctggaactg taatagagca aggcaatatt
    18721 gttatgtgcg tcaaggaaaa agctggtact actagattcc agaaaatagt aactatgatt
    18781 gaggaatctg aaaaactaaa atcttctgta gagagtaaat ttgaacatct agcagataca
    18841 ttagttccat acagcttttt aggaagtata ttaacctatg caattactag aaaccctata
    18901 aaatcactct ctatattaat ggtagatttc tcttgtgcat taaaactctc tatccctatt
    18961 tcagtactat cagccatgag agaatgtaac aataacaaca ttactgtaaa aggtggaaaa
    19021 tttcttgaag gtgttgcatg tgcagatact attgtatttg ataagacagg cacattaaca
    19081 aaagctcaac caactgtttc agatataatt acattccaaa attataataa ggaggatatg
    19141 ttaagacttg cagcttgctt agaagaacac ttcccacact ccatagcaaa tgcagtagtt
    19201 tatgaagctg aaaaacaagg gcttagtcac aaagaaatgc atacagaagt agaatatatt
    19261 attgctcatg gcatttcaac aaaagttgat gataaaaagg ttgtaatagg tagcaaacat
    19321 tttgtatttg aagatgagaa gtgtactatc ccagacggtg aggaggataa gtacaataat
    19381 ctatcagatg agtattcaca cctatttatg gcaatatcag gtaaattatc agcagttatt
    19441 tgcataaatg accctttacg aaaagaagca aaatatgtaa tttcaaattt aagagaatgc
    19501 ggaataaaaa agattgtgat gatgactgga gatagcgaaa aaacagctaa atctatagca
    19561 agtaaagttg gtgttgatga atattattca gaagtcttac cagaagacaa agccaatttt
    19621 gttaaaagtg aaaaactcaa aggtcgcaaa gtaattatga ttggtgatgg aatcaatgat
    19681 tctcctgcta tttcagaatc agatgtcgga attgcaatga gtgaaggtgc tgaaatagca
    19741 agagagattt cagatattac catatcagca gataatttga ataatttaat cgttttaaag
    19801 cagattagta ataagcttat gaaaagagtt gatttaagtt ctaagtttat tatcggcttt
    19861 aatttaggtt tgattttact tggtgtaggt ggatttgtta gaccaagtac atctgccttt
    19921 ttacacaacg catctactgt cggaataagt ttaaacagta tgacaaattt attggaaaac
    19981 accaatactt ataactacca ttaatctcca ttacataaaa catatgatat aaaaaattta
    20041 taattcatct ttttaaaaaa atatgtctta aaattagctt ttttctaatc aaaattatat
    20101 aaagcatact ttacaaattt taaactcaac ttatttctat tttttacaaa aaaggagatg
    20161 attcaaattt tcattttgaa acatcccctt ttgtaaattt ctttaaaatt tatatattaa
    20221 ttatattttc aaccaataat tgtattttca accaatcttt cttcatagca ttttaaatca
    20281 ttttgcttta gataattaaa ttcagactct acataaatac cacagcctac caattcaatt
    20341 gtcaaattag gtatattttc ctttagtata ctagtctgct cataaataat tctttccaat
    20401 aaatcaactt gtaatataga aaaatgcttc ataatctccc catcactatc tatatctttc
    20461 ttgatttcat accaactctt tgacaaaatt cccttcaaat caatataccc tagactcctc
    20521 aatatatcta tactatcgct ccatgcatac ttactaggtt tatcaaagta tgactctagt
    20581 ttcttcttct gctcatttgt aagtgaatca taagaaagct tcctattaaa atgtgccttc
    20641 aaagaaatct tagccataga atatggactt ctaccagagt ttaatcctgc atttttgaac
    20701 ttttcaattc ctttatcttc tactgaagac catattgagt ttattgaatc tgaatcattg
    20761 tatttaacat caagaccatg tgtaatattt atatagtttt gtggaaatac atattgataa
    20821 tagacatctt ttttatcatc atatagagag aagtatccac tatcatataa atgaatatgc
    20881 acctttactt ttaaactatc ctctaaaata tttcgtctaa ggttcgggat atttgcatac
    20941 atggttacat attttatggc atcaaagttt ttatttaatg gaacttcaaa actaatttca
    21001 tctcctgtca aaagcttttt cacttcttct tcattacaat catatagaga atatgtattt
    21061 tcaaatccca cacgtgattt cccatctaaa ctgcctgtta atgctacctt caaaatatgg
    21121 ttatctgttt tttgccattg gtaagcatct tggtcaagaa actctctaat ctgtctattt
    21181 tgctctttcg cctgatttat aacctcgttt tcatcttctg taatccctac atggcatcta
    21241 tttaatatat ttactatatt gcttccaaat gggaatgtcc atagttgaaa agctgctgcc
    21301 tcaacctgac acaagtatct aacctgattt aattcaattt gatggataag ttttttctga
    21361 tattcattca tctgattacc actatctgac atttgataaa tcaattctcc cattgcaaga
    21421 ttatcttttt tcttatcaat cttatcagta atgataattt gagtttccat catatcctgt
    21481 acttttttaa atattataga aatctcctct gaattttcca tttttaagct tccaaacttc
    21541 gtgcttaatt cagttaaaac ttccttcatc tttaatttac ttatctcatt ttcaaaaata
    21601 tcttgatttt taactgtact atcgaatttt tctacaaatg gtataacaga ttctttcaaa
    21661 gttttatctt taaattccac catttgagta ttcaattcat ctaatttctt aatattattt
    21721 tctttatctt taattgcctc tttaactacc tctattttac tctcaaaact agattttaca
    21781 ttaagacaat tattaacacc actaatcgca gtatttataa gagaacaagc agaacttcca
    21841 atactcaatg catttttaat tttagatgtc ttttgctgtc tttcaatagc ttgtttactt
    21901 ttctctagtg cagctaactt atctttttgc tgtttattat tgtaatttgc ctccaacata
    21961 cctatttctt ttttctggat ttttgtgtaa atttccttat actcgctttc acttaacccc
    22021 tcttttgctc tttgaatatc gtcagctttg tctgcatagt tatcatcatc tatagactta
    22081 actataatct ccttaatttg gtcaaattta gttcttcttt cttcatcaac acctgccaaa
    22141 ttataagtca ttttatctaa agcatttttt ctatcttcac ttagctgttc tccaagtaca
    22201 aatctcatac catcagaatc caatttaaaa tctgaaaaat taaccatcct gttagtccat
    22261 ttgtattcct cttctttaga tgaaagattt tccttcaaag attttgcact gtcaaatgcc
    22321 cctagacaag cttgaccaac accactaaat gcagtcagta aactcaatgc gctttgtatt
    22381 ccagaaagaa ttaactgtgt tttcatttgt tcctcaagtt ttgcctgttt cttttttagt
    22441 tcctctatgt cttcttgttc atcttctttc atctttataa tttcttgaat caacaggttc
    22501 atttttgcat taacagaaag cattttattc tcaagttcat tttttaaaac ctctatttgt
    22561 tgatttccag attcaatttg agttaggata ctttccttat atgtattgaa atatccttct
    22621 tttaactcat ctttcttccc ttgttccaat tctcttaagg tcttatccag ataatctgtc
    22681 aaagaacgaa tatctgtaac acgatttcct ccaatttcac tttcttcaat catctgcaca
    22741 taaaatgctg tcttctgctc aagtgcaaat aaaatcatac tccattcgct aaaattctga
    22801 ttttttccta caaaagattc tattctatca gactgcatca atttatttat aaggtattca
    22861 tagatttcct ttgcttcttt ataaaattct ttctttgtat tcatatcatc cagagaaagt
    22921 actgctttat ttttcttctc aaaactatag tacttctgaa gtatttctct atttaattgc
    22981 atctctattt tctcaactgc atgaaaaatt tcataaatat gtgaaagttc taaaatatca
    23041 gcttctaaaa ttcctactgc acacaacagt ctaagatttc tataaccttt aattccagaa
    23101 aaaccaaatc tctcgaaaaa ttcatagaag aatgtatcta ttgacaaaga atactcctgc
    23161 tttaacaatt ccttttcttt tagtgcatct ccagagtgac cagcttttcc ctctgctccg
    23221 gctgaacctg aatcttcacg tttgctaatt gtccagtcct tatgttcttc ataaagagta
    23281 tttaatttct ctccatcatc tgctgcttgc ttctgattta aagcatgatt aaacacatat
    23341 ctatcaaaat aatagtagtt tctatcacta ttatccattg gaggagaggt acctatatat
    23401 cttattgttt cttcatatat acctctaatt ccaccaagac ctcctgcacc accagctcct
    23461 ccagagcctc cttttcctgc taaaccatga tttccatctt ttttacttat agcacttttt
    23521 aataaaggag cttttccaag gagataaacc tgtccagact tgcttccaag acctgcattt
    23581 ccaccattac ctcctgtgcc tccagttcca ccttgttttc ctttttctgc ttttacaaca
    23641 tagtattttg tacctttaac attagcaaat tcttttgact cttttttaga acctatttta
    23701 gcatttgccc cagcttctcc agttatccct gtatctcctt tttttccatc ttgtccattt
    23761 ccaccacgtc caccatctac gtaaagttta agttctcctc ttttttctat ttctttaaaa
    23821 gataatatga tatcacctga agtacctgca ttattgccat ttcttccatt cattccgtcg
    23881 taaatagaac tttctccaac caatttttct gcatccttac cagatacgtt gattgaataa
    23941 gtcccttctg gtatatgtaa tttttctgta agtagtacaa tattcttacc tgagaagact
    24001 tctttctcca tattacagtc aattttcaac tcacaatctc catacatata tatagatgac
    24061 aaaatattaa gttgataacc agatattatt ttagtcaaat ccgataaatt cactttaggt
    24121 ataggaatat atatagaaag tacattttct ttctcatatg attttatata tgtacaactt
    24181 tgaatcttct ctatcctata ctctgcatct tcaagctttt tggcttcttc ttccctatat
    24241 tcttcttctg ctatccaata tttgatatct tttaatgtat cttctaaagt attttttagc
    24301 aatgatttta acaactcact tagaccaaca atatgagtct cactacagta actatttaac
    24361 tctaaaagaa gacgtgtttc ttctaatata ttcactttct gtacgccatc accatatgaa
    24421 agttcttgta caaactccat aagctctttc atattattag tcctactaca taaatctgta
    24481 tacatcttat ctaattgaat ctttttatct tttagactat ttatagaaga tagaatggaa
    24541 atagaatctt ccataccttt tttaacactt tctatataag gaactaaaag tttttctagg
    24601 tctttatagg ttacatcaac agctttttgt atttctattt tacaagaagc agtaagcatc
    24661 aatcccaaat ctgttttttc aaatgtttca ggtagatttt tcatttcatt caacaacttt
    24721 tctggctgtt tatctagtgg attacataat ttaatagata caccatctga taatgctaca
    24781 tttaataagt acactaattc cttataaccg tcgtaatctt tctcttgact atctaggtct
    24841 tcttttgctt ttttcaatat atttcttatt ttttcttctg ttacattacc gattagtgct
    24901 ttaggtattt tattgataag aaagaagaaa ttttctttat actttgcata atcaccaagc
    24961 atatctttca aatttttgcc tgtttctatt aataatgtat gtctagagtc cagtaaggat
    25021 tgatagtcta aaataactac catccctttc acctttgaag atgtagaaat agtttccctt
    25081 acaagcatag caccagcaat catatactct gctccacggt tatcaaaaaa acctgggcaa
    25141 tcacataatg aaaaatctct atttgcaata tctacaactt ctggacataa agtcttagaa
    25201 tatacaccat ttccaatttc gacagctgaa tcttttgcag ttatatatat ttgtccagtc
    25261 tcttcatccg ttttttcttc catcacacag ccttttaaat aatttataca agtactcttt
    25321 ccaacgcctg aagcacctag gaataacaaa attgactttt cttttaagtc atattttcta
    25381 agctcgtctc ttccttcttc caaaaggttt acgatttcac tataaagttt atttgccata
    25441 catccatccc tttctatatt tcggaacaat agccttgata tttttcttta ataagattct
    25501 cttctacctt tgaaacagct gttgataact tagtgtttgg agcattaaat cctccattgt
    25561 ctcctctatc tccatattca gcgtattctc ttgctggtcc tgttttacct tcattccaac
    25621 ttccaccact aaattctata tgatatttaa ttatactatc caacttataa tcccaacttg
    25681 taaaatatcc tgtatagtac acagtcttat ctataccact tggagaaaat gaaagtgtat
    25741 tgtatccgtg atagccacca cttcctccat atccacctga acctggtcta ccatttggac
    25801 ctgcttctcc atctttcact gttatatcac aatgattaat tattctattt ttttgttcct
    25861 ctgaatctga aatgaagtaa atattacctt tcttaccagc atttccacct cttccagcat
    25921 ttccaccagc tccacctttt tcaccttttt tgccacttgt accttttgca aaataccctt
    25981 taaacatctg tccgtggaaa taacttgtat accctgcatc agctccatta cttccagaag
    26041 ctcctgtttc accatttttc ccatctattc ctttagaacc atttcctcca tttgcaatca
    26101 gtgttaaacg ataatttcta atttcttctt taatatataa gtaaatatta cctgaatctc
    26161 taccatcttc accattttta ccatacatag aactatctac aagttctccg ctctttccac
    26221 ttacattaat ttttacatta ctacctacat caagattgtt acaagcaaga ataatatttt
    26281 tctctgatgc aaagtccatt ccaaggttat catccatata aatagtatct ttagcaaaga
    26341 acataaatgt agaaacattg tcatctactt ttctattttt gaaatattcc aatacactgc
    26401 taattactaa gtttggaatt aatgctctta tttcaatacc tttcttatca ttaatattaa
    26461 ttgtttttac tataaatgga tttaaaatgt atttaccaat aaattctcta attatctcat
    26521 taccttcttc tccatatgca cttaatcttg ttatactgtc ttctaactct tcattttgtg
    26581 taactgtaac atttgtcaaa attgaattaa aatcacttac actatataca taatcctttt
    26641 tatttgcaac ttccattaat ttttctctat ttggtaatgt aaatacctgt ttttgcactt
    26701 cataagaaac taaaactttt tctaaattct ttcttattat aatgaagttt tgttccatta
    26761 tcttcttact tgccttttct ataataaatt ccatatgcac agaaggtaat aaaatttccc
    26821 cagttccatg ttctgtatat ttacttaaag catcaattat atctaagttt ccttttatct
    26881 tattttgtaa tttcatgcaa ctatcttgta agaataagcc actttgtttg gctaatacgt
    26941 tgataaatct gtctatagta agacttgttg tactttccaa ccagttttta aatactttta
    27001 caacctcatc atattttgct atttcttgaa tcattgttat acttccaata atactgataa
    27061 aatcttctgt atacgcctta acataatctt ctaggctaga tacgatattg ttttgaattt
    27121 cttttatcaa cgtatctaaa actaatcttg catctgcact aattggatac tggaatgctg
    27181 atgtcttatc tttaacttca tctaattcta gtacttgctt taataaaccg tccctctcat
    27241 ctgatgcaag tggcttacaa agatgaatat tttttccatt atcttttagc aattcttcaa
    27301 ataaccaagc tttttcctct atattactgt ctaaattttc gcataattgt tcatattttc
    27361 tttttataac attatatatt tgtttttctg ttcttcctaa gaaacttttt gaaataaaaa
    27421 aagttatact acccttaaaa ttttctttat gaagacacat atttaactgt tggaacaggt
    27481 ctagtacttt tgtaagtcta gaatctaata tatctgcttc tggtatcaat acaattactg
    27541 cacatataga tgcagaattt ttaaatactt cacctaatga aactgctgcg ctaatatcat
    27601 gggctactcc cctattgtcg ctaaaacctg cagtatcaca taattttgca tccattcctt
    27661 ctattggttt tctcatattt acaatgttcg ggtataaagt ttgagaaaca gctcctgaac
    27721 caatctctgc cacagggtct aagcaatcaa tatatgatat accagtatct tcatcttcga
    27781 cttccatcat tacacatcct gcaagatagt taattgcagt acttttgcca catcctgtcc
    27841 caccaagaat tagaattaaa ttttgatttt cagccttatt tattgctact tttccttcat
    27901 ttaataagag tgttatttct tctaaaagtt gattgttatt ttctggcatt tattttcccc
    27961 cttggttata tattttattt aaataaagtt taatccctta agtagattac aaagttttta
    28021 gactatattt aaatttgttt tttatttttt atttttaata aatttgcatt ttcactaaat
    28081 ttgtattttc actcaataat tattcttctt tgtttattaa aataactgtg ttgtataata
    28141 taatctttac caacacagtt attctattta atgcgcttat tttattgtat tttatgtaat
    28201 acagttgttt tttatattat atgtccatta ttttcctatg gttattacat atagattttt
    28261 aattatattt gtaccattaa tttaaacatt cctatactac tgcttaagtt ttacacctat
    28321 accagcttga atattttatt tttatttgct taaatagttc aactatactg gtttaaatag
    28381 gttatcttta tttgcttaaa tagttcaact atactggttt aaataggtta tctttatttg
    28441 cttaaattag ttcaactata ctggtttaaa taggttatct ttatttgctt aaattagttc
    28501 aactatactg gtttaaatag gtttccttta gtttaaataa ttcacctata cctacttgaa
    28561 tagttcactt ttatttgctt aaattctata ccaatactgg cttaaacatt atactaccat
    28621 gattgtattg tctatcttca actacaataa aaacaggttc taaatcttta gtagatgtta
    28681 tttttaattc taaaaaaaca ttatcagttg gtaatatttt tacatcataa ttatttaaat
    28741 tattattagt gatactatta aatacatcac cttgacgttc aaaaaaaaca ttatcaacta
    28801 ttacaaaata aacatcttct tttctactat tttcaacctc tatagtattt tgtttagtta
    28861 catttagtct catcttccac ctcctattaa tattacttaa ttatattaat acttgtaatt
    28921 gttgcattat gttcatcaca aggaagaggt ttaaaaacaa ctatctctgc taaataattc
    28981 tgtggaggat ttgtaactga agtagcattg caaataaatt taaaatttgt tcctgctaca
    29041 acttgagtag aaacaataag tggttcataa ccaacccctt ctaacattcc tacagcttca
    29101 ttaaatactt ttaaatcttc ttcttcgaca gctctgaatt ttgaccatcc accaacaaaa
    29161 agactcatat aaaaacctcc tttatatata tatttattta tatatagtag taccaatttt
    29221 ctcaatattc aatattttct gtgtaactgg tcaatgaaaa tgcataactg gataaaaata
    29281 cttataaaat aacttaactt tacatataaa tagcttactt tacatgtaat aagattattc
    29341 tacaatatga aatacatctt gttttgaata tattctaaat acaataatgg atattttcaa
    29401 ttattatcct tagaaaatat ccattactta acttgtttta tttaaaatca catagagact
    29461 ttattatttt ttttattgca tgaatagcta tttcaggtat cataaggtga tataccccct
    29521 ccttgaaacc acaaaaaaca tgacaaactt tacgaatctt ggcttaaaaa actacaggta
    29581 cattccttat ttgtggattg gagtagccat aacaagcaaa ttttacaatt tttatgatga
    29641 caaaatcaat aaaatcgctt agtatataac acgaaacact acttgttgct attaagaata
    29701 gttagatagg aagtatgcat tataccaata aaggaatcaa taaagaaagt taaaacatcc
    29761 ctacaaatca aaaatctgtt acattttcta tatttaaacg ttatttactt acaattggag
    29821 aaataacagg ttttccttct cggtcaagta aaggcgtaaa tccggctgca tttccataat
    29881 agtggaatac atagttcaca cctgtttctt tatccaccca aatctccgtt gcatttattt
    29941 ttccttgtga atatactttt tcaaatctat cttttcccat tttataatcc cccatatttt
    30001 tgattggtct ataccttctt cacttaagtc gaaaaaatac ataacttatt tttttaattc
    30061 taatatgaac acgtaaaaat gtcaaacagc ttatttaatt tcataacttc atgttactgt
    30121 tcgtaacctt gtttgtttac ttacactaca ttacagcacc attttaccct ttacatgatt
    30181 gtattgcaca cagcaaaaat gcttacttta ttggattttt caactaaaaa aaggtttttt
    30241 tctccacaga acatttacta ttttttataa tatatctaat tttactctca tatcaacatt
    30301 taatgaagat ataacctttt tcaaatattg taaagactcc ttaaaaatat tagaaaaaga
    30361 ttctgcaaat ctctcaacat ctcctttttt taaataccta agtatctcaa aacatacatc
    30421 aaaacgtgtt ttcaaaattt cattatattc ttcatgaaaa aatctctcta aatagattcc
    30481 aaatatcata tattgtttta aatgcaaaaa tgtttcctgt aatacataaa tttgtgtata
    30541 atctactatc ttacaaagta aaacaaaatc tggataaacc ttacactctt cacaattatt
    30601 ttgtaagtac cttaaatgag tttctatttt atctacagct atcaaatcca ttttatttcc
    30661 tagtaaaagt actacatcct caagagtaat cgtaacaaac tccatagcat ccaaaagcaa
    30721 actcaacctt ttagctcctt cttctgtatt tatataatca tcatcatatt cagaaaaaag
    30781 tgttacatat gttcccttgc cattaattgt tttagcaatt cccaattcat ttagctcatt
    30841 ataggcactt ctaactgttg gaagtgatac attatatcta ttaattatcg aagtaattga
    30901 tggtagacaa tctcctattt taatattgcc ttcaaaaatt tgacaaatta catctgaaac
    30961 aattaagtca tataaataac atttttccaa cgttacagaa ttaaaactac ttgtatctat
    31021 actttgtgga gcacgatttt ctactatttt aagtatggat tctctacaag ctttatacat
    31081 atatgttaat agttttttaa attcaattag attcccgtcc aatccatata agtaagtttt
    31141 tcttacctgc tctatgtatt tattacgaaa aaagtctata caacaattat ccaagttatc
    31201 gaaattatag gccaattgtg aaataatgac atcatgctga ataatctcac aaaaatggtg
    31261 aatcaggaaa ttatttgttt tacttattag tttatttata tattttagcc tttgttcttt
    31321 aaagactaaa taattagaac tatttttttc catacaatcg ataatctcgt ttaattcttc
    31381 aaagtcttta gatgtaaatc ttttaatatt gtacatagca atagaaggaa atattaaact
    31441 aaaaatctta tatatttctg atatttgtaa aatcttctca tataataagt cagattctat
    31501 tgattgttta ctcgtactct ggattaaaac tggtctagaa ccagcttttg tctctataaa
    31561 gcccttttct gagagcattt ttattacagt ttttatagta ttttttgata ccatatattc
    31621 ttctgacatg ctttttatag aatcaagcct atctccaata gtaattttac cagtataaat
    31681 ttgttgaatt atatgctcaa aaataattaa atattgtggt tttctcttca tatttttcct
    31741 cttcgataaa ttagatttat tgttttttgt aaggtatatt tacttttatt taatagggca
    31801 aaatagttgc ttattttttc ctcataatct ataattaata taaaccattt atatatatgg
    31861 tctaagtttt ataatactgt aatgcagtaa aatagcatat tgtagctata ctacagcaaa
    31921 ttaacatacc attgtagtat attaattgga ataattttaa gtacttcaaa attccagcaa
    31981 aacatatatg tgtttttgat attatgtata ttcgttaata gaatagctat acttgtttta
    32041 cataataaat tatatgatac
//
LOCUS       DAELLV010000018        31553 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.018, whole genome shotgun sequence.
ACCESSION   DAELLV010000018 DAELLV010000000
VERSION     DAELLV010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31553
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.018"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(201..1040)
                     /locus_tag="KPD58_000738"
     CDS             complement(201..1040)
                     /locus_tag="KPD58_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG0264522.1"
                     /translation="MSDIKNEVSKKTLSKKDVIKSWLRWFFFAQSNYNYERLQSTAFS
                     HSMLPVLKKLYPDKEELKQEVETHLAFFNTEPICGCVIHGITIAMEEEKANGADEISG
                     DGMNAIKTGLMGPLAGIGDTLTQGVITPIVLAVCIGLTEGGASIAGPILFVIAQYIIM
                     TSISFGMWTNGYKYGKKAVESILQGGIVNKVIEGASILGTLVMGGLVGRFINLSTPIS
                     YTSGDFKFSLQTDLLDKIFPGLIPLVLTLLVLFALKKGLSPIKIMFILIVVGAITGIL
                     GLF"
     gene            complement(1064..1822)
                     /locus_tag="KPD58_000739"
     CDS             complement(1064..1822)
                     /locus_tag="KPD58_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0264523.1"
                     /translation="MHISLIQAVLIAIFYYLSWSPWLTYVGFFTWNRPLLAGFVTGII
                     LGDPVQGAIIGAGINMIYLGFISAGGAQMGDPAFAGYVGTALAIASKLDVSTAMAIAV
                     PLGTVATVLWIGKMTVNSFFAHWADREVQKGNVDRVAFINIVPPQILLFAMSFIPALL
                     VVYFGPNAIDGMLEVMNDNVLHVFNVIGAMLPALGIAMNLKLIGNKFTMPFFILGILM
                     AVYFKVDIIVISVIGVILALTITSIKYGKDSATS"
     gene            complement(1869..2342)
                     /locus_tag="KPD58_000740"
     CDS             complement(1869..2342)
                     /locus_tag="KPD58_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0264524.1"
                     /translation="MSNIALTRIDDRLIHGQVITAWCKITSAKRIVIVDDLVVKDPFI
                     VQVLQMAAPSTVKVEVHDVESGAEVLKSYNGDENLIVLVKYPKTVLGLVNSGVDLKEL
                     NVGGMGAGLGRKSFYKNISVSDEEKEIFRELISKDIKCFIQIVPDAKKIDVGTLL"
     gene            complement(2371..2787)
                     /locus_tag="KPD58_000741"
     CDS             complement(2371..2787)
                     /locus_tag="KPD58_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0264525.1"
                     /translation="MIHMAGIIIATHGNMAKAMLESAELIVGKQENVETLGLNHGDSI
                     DEFSRKLEDGIEKYKDEGVLVLLDFYGGTPFNTSAIIINKFAKVCELECLTGVNLPML
                     LELFLNRDTMKLKDLKNLCEEVGISGIKDVKTVLKL"
     gene            complement(2834..3886)
                     /locus_tag="KPD58_000742"
     CDS             complement(2834..3886)
                     /locus_tag="KPD58_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBG0264526.1"
                     /translation="MKSVRFYGIRDTRVEDVDVPKILEKDDVIIKVKVAGICGSDISK
                     YSKTGPHMVGEILGHEFSGEVAQVGKEVRSFKIGDRVAVCPAMPCFECNECKKGLYSR
                     CNNVAIIGNKELGGCFAEYTKVKERNLIKIPDEISYETAAALEPVCIAGHGLFRSEAK
                     VGDTVVVLGTGPIGLFSIQWAKIFGSTKVIAVDVFDEKLDLAKGLGADICINAKEKNI
                     VEEIKRLTDGDGADIVIESAGTPLTCGQVLLLAKKGGTVLYAGVPYGDVALTREQFEK
                     IVRSELTVKGTWFGNSFPFPGKEWSAGLYHMQKGDMNVEKLVTHRINLEEVPAYFEKV
                     YKRDIFFGKIMINIDN"
     gene            4282..4962
                     /locus_tag="KPD58_000743"
     CDS             4282..4962
                     /locus_tag="KPD58_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBG0264527.1"
                     /translation="MKKVYQGKTKDVFELDNGNYLLKFKDDVTGKDGVFDPGENSVGL
                     SIDGIGRANLETSVKFFEILNNAGIKTHYVSANLEEATMEVLPAKVFGNGLEVICRYR
                     AVGSFLRRYGSCVEEGAKLDCYVEATLKDDDRCDPLITSEGLEALNIMTQAQFDSMKS
                     MTQKISNIVRDTIAEKGLELYDIKFEFGFYNDEVILIDEIASGNMRVYKDGVIVDPMD
                     LTALLLDK"
     gene            complement(5095..5415)
                     /gene="sugE"
                     /locus_tag="KPD58_000744"
     CDS             complement(5095..5415)
                     /gene="sugE"
                     /locus_tag="KPD58_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HBG0264528.1"
                     /translation="MKWLFLFVAGLFEVFWAFQLKNSHGFSKLTPSILTVVGMIVSFY
                     FLSLALKHLPLGTSYAIWTGIGTVGTAIVGMAILNEPISISRIACIGFIVVGIVGLKL
                     LSTN"
     gene            5797..6624
                     /locus_tag="KPD58_000745"
     CDS             5797..6624
                     /locus_tag="KPD58_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBG0264529.1"
                     /translation="MNFYKIAVCQMITTENKIENINHAVDMVTEAAINGAKIVVLPEM
                     FNCPYENKYFPKFAEEYPGETTTILSKLAEKHGIYLVSGSIPELEDGKIYNTCYVFDK
                     NGDLIGKHRKMHLFDIEVTGKVSFKESDTLTAGNDVTVIDTEYGKVGIAICYDIRFPE
                     LSRLMALKGAEIVILPAAFNMTTGPAHWELSIRMRALDNQIFYVGAAPARNMNASYIA
                     FGNSRISDPWGRIIAQADEKECIIYADIDRDLIPDIRQQLPLLKHRRTDLYELNTLK"
     gene            7446..8120
                     /locus_tag="KPD58_000746"
     CDS             7446..8120
                     /locus_tag="KPD58_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0264530.1"
                     /translation="MNNKILVVDDEEGIITLLKDYFEMNSYEVYTAQSGKDALKKISK
                     NPDIILLDINMPEIDGIEVCNRIRDYVSCPILFLTARIENTDKINGFRAGADDYIVKP
                     FDLDELGARVSAHLRREQRKSNQTKIKFYNDLTIDYEKRLICIYNNQISLSKKEFDIV
                     ELLSMNPGQVFDKEKIYDRVWGYNSNGNSDVIMEHIRKIRIKLSKHTLENYIETVWGV
                     GYKWIK"
     gene            8108..9268
                     /locus_tag="KPD58_000747"
     CDS             8108..9268
                     /locus_tag="KPD58_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG0264531.1"
                     /translation="MDKINNMSLKKAFFVLTLSSLIIASVLTTFAYILLNNLYNSIQD
                     KYLKTPINTGIITIVQNNVNQFSDYDKTLLNIINILQLVLPLIFFIGLLLLADIIFYK
                     VKLKTPIEILNKGAMEISNNNLDFCLEYNNNDELGNLCNAFEKMRSELNKNNIKMWTM
                     INDRKQLNAAFSHDLRNPLTVLKGYSNYLTKYIPTGKLSDEKILSTTQLMSDHINRIE
                     YYVENMSNAQRLEDLVVSKSMSNINKFIENLDENISIIAKQEGKSFTLKSQINNINLF
                     FDENIILRVVENIISNAFRYARNNVSILIYLEQELLTFVIEDDGIGFSKESLKLALRP
                     FYRDKTLNDSNAHFGMGLYISKILCEKHGGSISIENNSTNSAKITAKFSTRN"
     gene            9539..10012
                     /locus_tag="KPD58_000748"
     CDS             9539..10012
                     /locus_tag="KPD58_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBG0264532.1"
                     /translation="MLSSTFCSLPFWICYFWCGISDIFDGLIARKLRQQSGIGAKLDS
                     IADFIFSVTILVTVGKNIALPMYLWWCIVAIALLRLISYSIGFFKYRTFSSLHTLMNK
                     ITGISIFAFPLLYICFGLDVAGIMICIVAFLSSFEEIAIMIKSKELNRDIKSIFM"
     gene            10298..10966
                     /locus_tag="KPD58_000749"
     CDS             10298..10966
                     /locus_tag="KPD58_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0264533.1"
                     /translation="MIIKAKQLSKIYGSNNNKVIALNNVNLEINSGEFVSVIGPSGSG
                     KSTLLHILSGLDNPTSGQVLLDDKDIYKHTEKELSALRRKSFGFVFQQFNLLPVLTAS
                     ENISMPVLLDKKQPDKEYLNEISSLLGIADRLNHLPHELSGGQQQRVAIARALIAKPD
                     IIFADEPTGNLDSKSGSEVMNLLIKTSKQFGKTLVVITHDDRIAKLADRKISIIDGVL
                     MEVK"
     gene            10969..13437
                     /locus_tag="KPD58_000750"
     CDS             10969..13437
                     /locus_tag="KPD58_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0264534.1"
                     /translation="MKNYLSLSIKELKSQKLMTTFIIIAIVLSTIMTTVVGQSIGILQ
                     NLRIEQARSFNGDRHVSFHQLTKNQVDDIKKDDRVYQAGTSITLGSSKIKDSGISVLV
                     KEYDKTGLSNYPKLMKLKSGNLPKDKNEIALDENTLKLMGIKPRLGVTIPMNLDISLL
                     NDTIPPYNYTANFKLSGILEDDYTGYVSGIVNGIVGKGTSENLLPKRYILSSLDFKIK
                     QQEKFQEIVNQLAKKINLSHNSIQYNWIYLNALKIQFEKDENSSNSDGISMIILVSLF
                     VALLVLLASGLVIYNILKISVTKKIKEYGCLRAIGAEPNQIYKIVILQILILCTVAIP
                     IGAVIGIISSKGITGMVTNILNPDILLANDNKEITELIHKNTTAYMFPLVLSTNVSLI
                     FSFISALPSAIYASHVSPKIAMVGSTTKIKRKIKREKTIKNFERHLAWLNLKRNKGRT
                     IITILSLFMSITVFVALSEFSNVLDVSRSVSNLKEGDFSLTNEISGFDKSTLDKITKM
                     KNVNRTSFIKYSEYKQGEIDTDINFENSGEMLKIIGIDEQTLKDLMPSITDSILEDFK
                     NGSICFIKNPIAISTPGVKTRHTNLKPKDSITINKKQLDIYSTVDKMFFLQGNGWING
                     VDVIVYDSVYNTLTNKNKFNQINIYAKDKSKLEQIRLSIEQICENNPGSHWISYIESD
                     KQLKESFKQIEFLAWAVILFVGLIGTLNIINTTHTNINTRTNEIGVKRAIGMSNSSLY
                     KMFLWEGVYYGIFAAIFGSIAGYASAIIINMATIEKLDFTNIPITSILQATIISVLAC
                     IIATLIPLRKVKKMNIIDCIDINL"
     gene            complement(13677..15101)
                     /locus_tag="KPD58_000751"
     CDS             complement(13677..15101)
                     /locus_tag="KPD58_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0264535.1"
                     /translation="MKNQSLITQFRHTFIFIIIASIVATVITYVFALYLYIHSLNKDI
                     YPPNYYERQVPRIEKYINEKNITLLSQSNEEGLKRTIRGDDMLYQVVDNNGNILYGTN
                     PKKLFKTKEELFNNFINKTVRKGGYIHTVPIKGDNGKIEGAVILFYQVKITFANIRGR
                     FVFAVIIMALFSPFLYIVGFTRWLSKRFVKNINQPLHLLIDASKKIKEKDLDFEIDYY
                     SDNELGKLCSAFSEMKDELKGSLSAQWKMEQERVEMVEALAHDLKSPLSIILGYTDAL
                     IGNNTDDNEKLHRYLTVIRENTEKSAALVQKMQYTSDLEKSNIQLNLVPINLPEFLRQ
                     KVQDYELQAHQKEVELILKMQGNIQSPIQIDVDRLTRIFDNIISNSLQYTPSGGNISI
                     TVKDEKNCISYEICDSGRGFSSKDLKKALDKFYRGDEARQTKGGHSGLGLYIVKQLVE
                     QLGGSVKIENSKSGGACVKFWHSI"
     gene            complement(15120..15812)
                     /locus_tag="KPD58_000752"
     CDS             complement(15120..15812)
                     /locus_tag="KPD58_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0264536.1"
                     /translation="MSKILIIDDEMDLVMLLEDELKAKGHEVLIAYDGQAGIELSKQE
                     PDLIILDIMMPKMNGFEVCQAIRDDVLCPIIFLSAKQSETDKIKGLTFGGDDYVTKPF
                     GLRELMARIEANLRREKRSQYINEENKRSTLYFGKLCLYMKERAIKIDGKNIDLTKIE
                     YDIVELLALHAGQVFSREQIYEKVWGCDSEGDSSTVVERVKKIRAKFSAVTPEKEYIS
                     TVWGIGYKWNKM"
     gene            complement(15867..16643)
                     /locus_tag="KPD58_000753"
     CDS             complement(15867..16643)
                     /locus_tag="KPD58_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HBG0264537.1"
                     /translation="MIKLLSSEWLRAKRTAVQWLTLCMPIIFSLCVVFYLTTKSGSTQ
                     EFAFEGFFTVWTVFIIPIGVGILAGFIVQEEELAGNFNGFLCVGISRVRLYLGKFLFL
                     LFCLTICTFIATLILCIGMSIAVPSGATIWLFLSAAGLVVIGTLPLLAIHLWVSFAWG
                     MGASIGISIGGILMAAILGLTSVGEKVWAFVPWTWPVKLGMLPGIYFIKEAGTISTEA
                     FYSKVMQTASIELIVVAIGLIIFLIGGVIWFKMWEGRKSY"
     gene            complement(16640..17410)
                     /locus_tag="KPD58_000754"
     CDS             complement(16640..17410)
                     /locus_tag="KPD58_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HBG0264538.1"
                     /translation="MNALQSELLKYKRTFMGKLIVFFPVSFAAYAFIMQSTLMQNPLS
                     QTTSWAWQNLLALIFNWWSFLFLPIGFALFATLVAFQEKKAGNYRALRTHNVSPMTLW
                     INKVIAMAVYSFISTLVLIVVTIITGLILKAGPVPFGQIIGASIVCWVVSLAILPLQL
                     WLATWKGMFLSMGVGALGMIFGVLAATKPFWIAVPWSWAVRMVCPIINIHPNGTILEA
                     GDPLLNTSVIPIGIVVSLVVFIVLTALTAAWFNRRDDK"
     gene            complement(17407..18111)
                     /locus_tag="KPD58_000755"
     CDS             complement(17407..18111)
                     /locus_tag="KPD58_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HBG0264539.1"
                     /translation="MKDLILETKNLSKRYGEQMAVNNVSLQIERNSIYGLLGPNGAGK
                     STTLKMLVGLLRPTNGRIIFDRKPWKRESLAKIGSLIESPALYGNLTAEENLLVHTKI
                     LGIPKDKIYEVLEIVNLKNTGKKRASQFSMGMKQRLGIAIALLSDPELLILDEPTNGL
                     DPFGIQELRELIASFPKKGITVILSSHILSEVAQVVDNIGIINGGRLLFQGMPDPNEN
                     LEEFFTDVILKGGQNR"
     gene            complement(18366..18878)
                     /locus_tag="KPD58_000756"
     CDS             complement(18366..18878)
                     /locus_tag="KPD58_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264540.1"
                     /translation="MKKISQEKISTHIQEIPQEKIEQWKSLYEKYKSILKVNSKSVLE
                     IIEFLKRKYVIVEETSEEFKHVVINNIKLNKPFSQKISSDKELKPIVFTIPNEGNAKK
                     LYDTQEEIYSGCKIIVGMEFETGCVFVEGSDELNDEITSFKGLDKYDINNYYLVANYI
                     RCLKKYKLID"
     gene            19204..19461
                     /locus_tag="KPD58_000757"
     CDS             19204..19461
                     /locus_tag="KPD58_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264541.1"
                     /translation="MILNRAELFRGYSLNRFNEICNILSVNKIKYKYKVKNNAYSKKP
                     FFNEITLGNLGQKEDFSYEYLIYVHKDDYEQAESLINSKLR"
     gene            19604..20230
                     /locus_tag="KPD58_000758"
     CDS             19604..20230
                     /locus_tag="KPD58_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HBG0264542.1"
                     /translation="MKINIKSKLSEFIKQNNLFDDSRIISYLPNITIETDKIKTIMIN
                     EVVPTNTNDDFYSVDKDADYLKTTIPLFDSAGIKVSNINDILDMGIYITNAVKLPKSE
                     YTITRDTIKLHMPILEEEIKLFKNLEVVMLMGDVAKKSFNMITKKHIKKNVIPSISTY
                     KLRNNELYYDNLRVFPSYIMTGGNILIEKSKFEMASEDIKKMFEIING"
     gene            complement(20477..21256)
                     /locus_tag="KPD58_000759"
     CDS             complement(20477..21256)
                     /locus_tag="KPD58_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901940.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBG0264543.1"
                     /translation="MNSKIYYFSGTGNSLVVSNILKETLNGSKEVIPLAIHRKENNID
                     INEDILVIVFPVYFADVPDIVKSFVEKLNFNINTHIYAIATCNGVAGHSLFTIDKLLK
                     KRGKKLSAGFLINMPGNALITPPDVEEERLKNQTNRVAEIAKCINNLEIGNIEGKNNL
                     KSHIDSVVLRLVGKNMQIATTKFFAENKCNGCGTCKRVCPVENISIVDKKPKWGNECE
                     RCLACFHWCPKEAINVKKSFLTNRKKYHHPDITVKDIFLDK"
     gene            complement(21324..21785)
                     /locus_tag="KPD58_000760"
     CDS             complement(21324..21785)
                     /locus_tag="KPD58_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0264544.1"
                     /translation="MGGARDYLNGGKMKDFEKLYEATTKLVSTSLKVKDDLKKMFKQN
                     GYDITTDHYALLRFLWEQDGISQIDLCEKSCKDKSNTTRILDVMKNKGLIVRKVDVKD
                     RRKFQIFLTDLGRELEEPLNEIASIYATETFKSISDEELPLFTDILDRIGK"
     gene            complement(21761..22438)
                     /locus_tag="KPD58_000761"
     CDS             complement(21761..22438)
                     /locus_tag="KPD58_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG0264545.1"
                     /translation="MIITVINGSPRKNGATSKVLTYLYKDIERLIPDVKINYFDLSEV
                     NPSYCIGCLNCYKMGKCINQNDKVEYIHDIITKSDGVIFGSPTYGSSVTGLFKVFTDR
                     AHMMLERLLYRKPCIAVTTYENARGSKAISFIKSMVLDSGGYVCGSLSIKTGFNQNPI
                     TEKVESKIQKVSKKFIYCIEEKKNPPVLSQIYNFIAINAVLKPMAFKDIEQYKGIIDR
                     WEEQGII"
     gene            22671..23057
                     /locus_tag="KPD58_000762"
     CDS             22671..23057
                     /locus_tag="KPD58_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0264546.1"
                     /translation="MKKLPHISEAEYQVMKIIWKYAPISTTEVIEKLVETSTWSPKTI
                     QTMLLRLVKKGALTYEKNSRVFVYTPLVKEEEYVARESSSFLNRFYNGTLNSMVLNFL
                     ENDKLSEDDIEELREILNKRTTKGDK"
     gene            23060..24859
                     /locus_tag="KPD58_000763"
     CDS             23060..24859
                     /locus_tag="KPD58_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaR1 family beta-lactam sensor/signal
                     transducer"
                     /protein_id="HBG0264547.1"
                     /translation="MYAPFFIRFLLSTLTLSILIVVILLAKKVFKKHISAKYQYNIGF
                     ILLIMLIIPFVPLKYINLGNVFNYLYTFNQLGSSTNRTSMIKNSPNSVINNSNALNDF
                     SVSLNQVNFDSLNILLLISWIIGILIMIAITIHCNIKIKNMKKSIQILRDDEIVFIFE
                     KCKKDIGINKDFILCKSPIINTPITFGYFKPHIILPDKSISKLSLKDIKYILLHELQH
                     FKNKDILINYIMCFLQILYWFNPLVWYAFKEMRIDREIACDISVLKKLDKDSHIEYGQ
                     TLINFVDNISRPSNLTLTSDIGGSKKQIKKRLLKIMDFHNESNTLKFKSILIFTLIAL
                     IVFGNIPKLSIMADINEKYRPTNEQTVYEDLSQYFKGFDGSFVMYDLKSKQYHIYNKE
                     KSKTRISPNSTYKIYSALLGLENGIITRNNSHIPWDGKHHSNSSWNEDQDLFSAMENS
                     VNWYFQTLDKKTGLKNIQTYLRQIGYGNYDVSGGISNFWLESSLKISPIEQVELLESF
                     YTNKFKFEDKNIETVKDSLLISKKDGVSLFGKTGTANINGKDVNGWFIGYVEKNENTY
                     FFATNIQSKDYSNGSSASKITLSILNDKKIYNP"
     gene            complement(25214..27088)
                     /locus_tag="KPD58_000764"
     CDS             complement(25214..27088)
                     /locus_tag="KPD58_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG0264548.1"
                     /translation="MNKYNKIANELIKIIGEDNIISITHCATRLRVMVKDREIINDKK
                     VEKVDEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLLGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEIEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIDLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLVSIFVVA
                     FCFIVTCLFGVPKEAINEDEDEELVFNENNENFVSPAKGKIVALENVPDETFSKKMLG
                     DGFAIDIIDGKIVSPISGKLETVVSSGHAFGIKGTNGEVLIHVGIDTVALNGDGFDVA
                     VKQGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE
                     "
     gene            complement(27093..28757)
                     /locus_tag="KPD58_000765"
     CDS             complement(27093..28757)
                     /locus_tag="KPD58_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBG0264549.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIISRLDYLKDLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKESKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKTVRNQPGIEKMLYELKENTFKKYDCMTVAEAAGISYDQLGDFIGEDGFFSMV
                     FDFKHADLDVASGSEWFKRISWTIPDLRKCILDSQMYVQEHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRDTINDFNDLSSIDQYY
                     RSIDEGYSEEEALEFVNLRSRDNARTPFPWADKENAGFSDTTPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSEYSDCLIYGSIEPIDIESEDVIAYRRQLDLVVIDCY
                     YNFSDKPVEIRVEEKYDIVFQNLDDINLLNKTVCLKPFHAVLLKAR"
     gene            complement(28771..29592)
                     /locus_tag="KPD58_000766"
     CDS             complement(28771..29592)
                     /locus_tag="KPD58_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG0264550.1"
                     /translation="MKLYKLLILDIDGTLRDEVYGIPESAKHAIRLCQKNHCSVVICT
                     GRSMGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKEVVCLLKKREVAF
                     SIESQEKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDIHKIC
                     LWSNEKVFDEVKDILQDKMELAQRDISSQYYEIIQKDFHKGKAIKRLQERLGVTQKET
                     ICFGDGQNDIVMFQASDVTIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            complement(29691..30455)
                     /locus_tag="KPD58_000767"
     CDS             complement(29691..30455)
                     /locus_tag="KPD58_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG0264551.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVIDDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVAKEMKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDASTIIDFAKEMRMKGIYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            complement(30452..31201)
                     /locus_tag="KPD58_000768"
     CDS             complement(30452..31201)
                     /locus_tag="KPD58_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG0264552.1"
                     /translation="MRLESLVNQNYDNLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYDAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KSDLFIIISLSGETKAGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
BASE COUNT        11169 a         5223 c         3823 g        11338 t
ORIGIN      
        1 gaaaaagcac ctcattctga ttaaaaatct ttctgagata ctttttaaaa tattaaatac
       61 tacatatagc ttacataagt ttaaaattta atctattata aaattaccct aattgccaaa
      121 ctttttaata taaattttcc aacatcctac aaatataatc aatagatata gaatcctaac
      181 ttacaccaat atatatttat ttaaaatagt cctaaaatac ctgtaatagc tcccactact
      241 atcagtataa acattatttt tattggagaa agaccttttt ttaaagcaaa gagtactaat
      301 aaagtaagta ccaaaggaat taatcctgga aatatcttat caagtaaatc tgtttgaaga
      361 ctaaatttaa aatcaccact agtataactt attggtgtag ataaattgat aaacctacct
      421 acaagccccc ccatgactaa ggtacctagt atagaagccc cttctataac cttatttact
      481 attccacctt gaagtatact ttcaacagct tttttcccat acttatatcc atttgtccac
      541 attccaaaag aaattgaagt cattattata tattgagcta ttacaaataa tataggtcct
      601 gcaattgatg ctcctccctc agttaaacca atacaaacag ccaacactat aggagttata
      661 actccttgag ttaaagtatc gccaattccc gctagtggac ccatcaagcc tgttttgata
      721 gcattcatac catctccact tatttcatct gctccattag ctttttcttc ttccatagct
      781 attgttattc catgtataac acatccacat ataggttctg tgttaaaaaa tgctagatga
      841 gtctccactt cttgttttaa ctcttcctta tctggatata attttttaag tactggcaac
      901 attgaatgtg aaaaagctgt tgattgcaat ctttcataat tgtaattaga ttgtgcaaag
      961 aaaaaccatc ttaaccatga ttttattaca tctttcttgc ttaacgtttt cttagatact
     1021 tcatttttta tatctgacat aatattttcc ccctcacatg tatttaagat gtagcactat
     1081 cttttccgta cttaattgaa gtaattgtaa gagcaagtat tactccaata acagatatta
     1141 ctattatatc tactttaaaa taaacagcca ttagtattcc taatatgaaa aagggcatag
     1201 taaacttgtt tccaatcaat tttaaattca tagcaatccc aagtgctggc aacatagccc
     1261 caattacatt aaatacatgt aacacattat cattcattac ttccaacata ccatctatcg
     1321 catttggacc aaaataaaca actaataatg ctggtataaa actcattgca aataacagta
     1381 tctgtggtgg tactatattt ataaatgcta ctctatcaac attccctttt tgaacctctc
     1441 tatcagccca atgtgcaaaa aaagaattta ctgtcatctt tccaatccaa agtacagttg
     1501 caacagttcc caatggtaca gctattgcca ttgcagtact tacatctagc ttagatgcta
     1561 tagctaaagc agtacctaca tatcctgcaa aagctgggtc ccccatctga gctccccctg
     1621 ctgatataaa acctagatat atcatattta tacctgctcc tataattgct ccttgaacag
     1681 ggtctcctaa tataattccc gttacaaatc cagccaatag aggtctattc catgtaaaaa
     1741 atccaacata agtcaaccac ggactccaag aaagataata aaaaattgct attaatactg
     1801 cttgaattag tgaaatgtgc atattcttcc tccctatttt cttttaaaga tttacaagat
     1861 agtcttcttt ataataatgt tcctacatca attttctttg catctggtac tatttgtata
     1921 aagcatttaa tatcttttga tattagctct ctaaatattt ctttttcttc atctgacaca
     1981 gatatatttt tataaaaact ttttctacca agccctgctc ccatacctcc aacattcaat
     2041 tcctttaaat ctaccccgct atttactaaa cctaagacag ttttagggta tttgaccaat
     2101 actattaaat tttcatctcc attatatgat tttaagactt cagcacctga ttctacatca
     2161 tgtacttcaa ccttaacagt tgatggagct gccatttgta aaacttgaac tataaatggg
     2221 tcctttacta ctaaatcatc cacgattact atcctttttg ctgatgtaat cttacaccaa
     2281 gcagtaataa cttgcccatg tattaatcta tcatcaattc ttgttaatgc tatattactc
     2341 ataattattc ctccagattt ttaatttatt ttacaacttt aaaacagttt taacatcttt
     2401 gattcctgat atcccaactt cctcacataa attcttcaag tcttttaact tcatagtatc
     2461 tctgtttaaa aataactcta aaagcatagg taaatttaca cctgtcaaac attctaactc
     2521 acatacctta gcaaatttat ttattataat agcagatgta ttaaatggag ttccgccata
     2581 aaaatcaagc aatactaaaa ccccttcatc cttatatttc tcaattccat cttctaactt
     2641 tctactaaac tcatctatgc tgtcaccatg gtttaatcct aaagtttcta cattttcctg
     2701 tttgcctact attaattcag cactttctaa cattgcttta gccatatttc catgtgtggc
     2761 tataattatt cctgccatat gaatcatacc tttcttaacc ttttttatat ggattattat
     2821 ttagtactct aatctaatta tctatattaa tcattatctt accaaagaaa atatcccttt
     2881 tgtatacttt ttcaaaataa gctggtactt cttctaaatt tattctatgt gttactaatt
     2941 tttcaacatt catatcaccc ttttgcatat gatacaatcc agcactccat tcttttcctg
     3001 ggaatggaaa ggagtttcca aaccaagttc cctttacagt taattcactt cttactatct
     3061 tttcaaattg ctctctagtc aaagctacat ccccatatgg tacacctgca tacaatactg
     3121 tacctccctt tttagctagc aacagtactt gaccacatgt cagtggagta ccagccgatt
     3181 caataacaat atctgcacca tctccatcag ttaacctttt tatttcttct acaatattct
     3241 tttcttttgc atttatacaa atatctgccc ctaacccttt tgccaagtct aacttctcat
     3301 caaatacatc cacagctatt acttttgtag aaccaaatat ttttgcccat tgtattgaaa
     3361 ataatcctat tggacctgta ccaagtacta caacagtatc tccaacttta gcttctgacc
     3421 taaaaagacc atgtcctgct atacatactg gttctagcgc agcggcagtc tcataactta
     3481 tctcatctgg tatttttatt aaatttctct ctttcacctt tgtatactct gcaaaacaac
     3541 ctccaagttc tttatttcct attattgcta cattattaca tcttgaatac aatccttttt
     3601 tacattcatt acattcaaaa catggcatgg ctggacaaac agcaactcta tctcctattt
     3661 taaaactcct tacttcttta ccaacctgtg ccacttctcc agaaaattca tgccctaaaa
     3721 tttctccaac catatgtgga cctgtctttg aatattttga tatatcagaa ccacatattc
     3781 cagctacttt tactttaatt attacatcat ctttttctaa tatttttggt acatccacat
     3841 cctccactct tgtgtctctt ataccataga atctaactga cttcactatt aaccacccct
     3901 actttttatt tgagaatata tccctattgt ttatatgtta aatatctaaa gcaactttca
     3961 tgccaatata tgtaagcatt gtattcatgc attttataaa attatcatta aatgtgtgtc
     4021 aaagtgtgtc aaaagtttct tatattaaaa cttatgtgtg tcaatataaa cgacactaac
     4081 attttatatt agttctctta ttataagttt tataaaatgt actttttttg ttatttttat
     4141 aaaattgttc ggaaattccc cttctatttg tgatataata ttaaatataa ggtatcaata
     4201 taaattttca tgcgaatatc agaacttttt tgacactaat gccttattca atataataat
     4261 ttaacttttt ggaggttgtt tatgaaaaaa gtgtatcaag gaaagactaa agatgttttt
     4321 gaattagata atggaaatta cttacttaaa tttaaagacg atgtaactgg taaagatgga
     4381 gtatttgacc caggcgaaaa tagtgtaggt ctaagtatag atggaattgg tagagctaat
     4441 ttagagacat ctgttaagtt ctttgaaatt ctaaacaatg ctggaattaa aactcattat
     4501 gtaagtgcta atttagaaga agcaactatg gaagttctac cagcaaaagt ttttggtaat
     4561 ggattagaag ttatttgtcg ttatagagct gttggtagtt tcttacgtcg ttatggttca
     4621 tgtgttgaag aaggtgctaa actagattgt tacgtagaag caactttaaa agatgatgac
     4681 cgttgtgacc ctcttataac atctgaaggt ttagaagctt taaacattat gactcaagct
     4741 caatttgatt caatgaaaag catgactcaa aaaatttcta acatagttcg tgatactata
     4801 gctgaaaaag gtcttgagct ttatgatatt aaatttgaat ttggattcta caatgatgaa
     4861 gtaatcttaa tagatgaaat agcatctgga aatatgcgtg tttataaaga tggtgtaata
     4921 gttgacccta tggatttaac tgctctttta ttagataaat agtatataca tataatttac
     4981 aaatataata aacttataaa taagataagc tatgaagtaa agacttttta aatgttgatt
     5041 atttttaaac attttctcgc ctttaatttc atagcttatt taaattaata tactctaatt
     5101 agtagataat agctttaacc ctacaattcc caccacaata aaacctatac atgcaattct
     5161 tgatatacta ataggctcat ttaaaatagc catgcctaca atagctgtac cgacggtacc
     5221 aattccagtc catattgcat atgaagttcc aagtggaaga tgttttagtg ctaatgacaa
     5281 aaaataaaaa cttacaatca ttccaacaac agtaagtata cttggagtta atttagaaaa
     5341 tccatgtgaa ttttttagtt gaaaagccca aaacacttca aacaaacctg caacaaacaa
     5401 aaataaccat ttcataaatt catccctctt ttcaaatatg tctcttattt ttccaataaa
     5461 ataacctgag aaatattata aaatatctcc caggtttttg tccttccgtg ttcacaacaa
     5521 aaactatgtt gtgggtttta tctcggacca gaccaatcat taataattgc ggaaccctat
     5581 aaaactctat taattattct attttatcta taataatact atcaacatat ataaaaaaag
     5641 tcaatatatg ggttccatat ataataaaaa taacttttat atacctttta atattcttat
     5701 tcacttctct ccatacccat ttgggtcaat ctaagaatat ctaattttta tttaatatgt
     5761 tataataaca taaaaatatt gaaaggagta atttctatga atttctataa aattgcagta
     5821 tgtcagatga ttactacaga aaataaaatt gaaaatatca atcatgctgt agatatggta
     5881 acagaagctg ccatcaatgg agctaaaatt gtagttttgc cagaaatgtt taactgtccg
     5941 tatgaaaata aatattttcc caaatttgct gaagaatatc caggtgaaac aacaacgata
     6001 ttaagtaaat tagctgagaa acatggaatt tatctagttt ctggtagtat tcctgagtta
     6061 gaagatggaa aaatctataa tacttgctat gtatttgata aaaatggaga tctaattgga
     6121 aaacatcgta aaatgcactt atttgacatt gaagttacag gaaaagttag ttttaaagaa
     6181 tcagatactc ttacagctgg aaatgatgtc actgtaatag ataccgaata tggaaaagtt
     6241 ggtatagcta tttgctatga tattcgtttc cctgagttat cacgtttaat ggctcttaaa
     6301 ggtgctgaaa tcgttatctt acctgctgca tttaatatga ctactggtcc cgcccattgg
     6361 gagttatcta taagaatgcg tgctttagat aatcagatat tctatgttgg tgctgcacct
     6421 gctagaaata tgaatgcttc ttatatagct tttggaaatt caagaatttc agacccttgg
     6481 ggaagaataa ttgctcaagc tgatgaaaaa gaatgtatta tttatgctga cattgataga
     6541 gatttaattc ctgacataag acaacagtta ccacttctta agcacagaag aactgattta
     6601 tatgaactta atactttgaa ataaaacaga acataacccc ataaatcata aattaagtgt
     6661 taaaaaaatt tatggggttc tcattaattt gatttaaaat taatttcata aaaataatgc
     6721 taaaattaat ttaattaaat aatactaata attactaatg attatgctaa ttatcagcat
     6781 ttactaattt aatcactatt agttatttct tttattaaat atctttactt ttttacttat
     6841 tatgtcttct agctttacat ttggattatg gtatttcttt ctatttttag tcacatcttt
     6901 ctattgaatt acttgcttaa aacaccaatg cacacatgcc agataatgtt cacaattatg
     6961 tagaaatctt gctctacctt cttcaatgtt gatattatct ctcctagttg atattatctt
     7021 tcctaaaatt tcttttaatt aaaaaataac attagttgat atatcaagta tattttaatc
     7081 aactgaaaaa aacaataata attgatatat caactaatta atgtagatta attcattttt
     7141 ataatttata tactttttaa ttaatctacc ttatttaatt aaaatccttc ctttctattt
     7201 atccattttc aacttaaatt ataagatttc atcagctata tttttgcata ccaataatta
     7261 ttaaatatca aatatatatt ttattaaaat ttctaataat ttcatccttg atttatttaa
     7321 tatataaaaa tatactaact ttaatgatta agttttgaaa tataactaag aatttgataa
     7381 tttattgaga tttttatact aaaatataca tactatacaa acttaattta gaagggagca
     7441 ttcttatgaa caataaaata ctcgtcgtag atgatgagga aggtataata acactcttaa
     7501 aagattactt tgaaatgaat agttatgagg tttacactgc tcaaagtgga aaagacgcat
     7561 taaaaaaaat atccaaaaat ccagatatca ttctacttga tataaacatg cctgaaatag
     7621 atggaattga agtttgtaat agaattagag attatgtatc atgccctatt ctatttctta
     7681 cagctagaat tgaaaatact gacaagataa atggttttcg tgctggtgct gatgattata
     7741 ttgtaaaacc ttttgattta gacgaacttg gagctagagt atctgcccat ttaagacgtg
     7801 aacaaaggaa gtcaaatcaa actaaaatta aattttataa tgatttaaca atagattacg
     7861 aaaaaaggtt aatttgtata tataataatc agatttcact ttcaaaaaag gaatttgata
     7921 tagtagagct tctctctatg aatcctggac aagtatttga taaagaaaaa atatatgata
     7981 gagtctgggg atacaatagc aatggaaata gtgatgttat aatggaacac attagaaaaa
     8041 tacgaatcaa actatcaaaa catactcttg aaaattatat agaaacagtc tggggagttg
     8101 gttataaatg gataaaataa ataatatgtc attaaaaaaa gctttctttg ttcttacttt
     8161 atcttcacta attatagcat cagtgcttac tactttcgca tacattttac ttaataatct
     8221 atataattct atacaagata agtatttaaa aacacctata aatacaggta ttataaccat
     8281 agttcaaaac aatgtgaacc aatttagtga ttatgataaa acattactta atataatcaa
     8341 catacttcag cttgtacttc cacttatatt ttttatagga cttttattat tagctgatat
     8401 aattttttac aaggtaaaat taaaaacacc catagaaata ctcaataaag gtgcaatgga
     8461 aatatccaac aacaatttag acttttgctt agagtataat aataatgatg aattaggtaa
     8521 tctatgtaat gcttttgaaa aaatgaggtc tgaactaaat aaaaacaaca taaagatgtg
     8581 gactatgatt aatgatagaa aacaacttaa tgctgctttt tctcatgatt taagaaatcc
     8641 cctaacagtt ctaaaaggtt actcaaacta tttaacaaaa tacataccaa caggtaagct
     8701 tagtgatgag aagattttat caaccacaca acttatgtct gatcatatta acagaattga
     8761 atactatgta gaaaatatga gtaatgctca acgattagaa gatttagttg tttcaaaatc
     8821 tatgtcgaat ataaacaagt ttatagaaaa cttagatgaa aatatatcta tcattgctaa
     8881 acaagaagga aaatctttta cacttaaaag tcaaataaat aatataaatc tattttttga
     8941 tgaaaatatc attcttagag tcgtcgaaaa tataatatcg aatgctttta gatatgctag
     9001 aaataatgta tccatactta tatatcttga acaagaactc cttacctttg taattgaaga
     9061 tgatggtata ggatttagta aggaatctct aaaattagct ttaagacctt tctatagaga
     9121 taagacttta aatgacagca atgcacactt tggtatgggt ctttatatct caaaaatatt
     9181 atgcgaaaaa catggtggct ctatttctat tgaaaataat tctactaaca gtgctaaaat
     9241 tactgctaaa ttctcaacaa gaaattaaaa tgattttgta agctttcact gttcttatat
     9301 aaaatttgtt agtttagaat ttataaaatg tatttcaatt tacaaccaat atatatacta
     9361 ttttgtagaa gttgtaagag ttgcttcaat tataatattt tggcaaaagg catttgaaac
     9421 aataatagaa atatatattt aaatatgtag aatagtatga aattagaatt ttggaggtga
     9481 ttatattaaa agttttgcta ataatattac attctctcgt attgtgtttg cattgctaat
     9541 gcttagttct acattctgtt cactgccttt ctggatatgt tatttttggt gtggaattag
     9601 tgatatattt gatggtctta ttgcccgaaa gttgagacaa caaagtggaa taggtgcaaa
     9661 gttagatagt attgctgatt tcattttttc ggttacaatt ttggttactg tcggaaaaaa
     9721 tattgctctt cccatgtatc tatggtggtg tattgttgca atagcattgt tacggttgat
     9781 tagttacagt attggttttt ttaagtatcg cacattttca tcgcttcata ctttgatgaa
     9841 taaaatcact ggaatttcta tctttgcgtt tccattactc tatatttgct ttggtcttga
     9901 tgttgcagga attatgatat gtatcgtggc ttttctttct tcttttgaag aaatagcaat
     9961 tatgataaaa tcaaaagaat taaatcgtga tataaaaagt atatttatgt gaaaacttag
    10021 aatttgtgac aaaaataatt tatataataa tgaggaagta aaacataaaa aattatccac
    10081 aagtttatgt gtaaaatagt tttaaagcaa tagagttgta ttgtaaagcc tttagtgcat
    10141 aaattacatt ttaaattaaa aatgatactg aaactgctta tcagcactgc gaattatccg
    10201 caaatagtga agacttttta gatttaagct aaaagttggg aaaaaattga gttttaaaac
    10261 ttacactctt cttatataga aaaggagagt gttttttatg attataaaag caaaacaatt
    10321 atcaaaaata tatggttcta ataacaataa agttatcgca cttaataatg taaatttaga
    10381 aataaactca ggtgaatttg tttctgtaat tggaccttct ggaagtggaa aaagcacact
    10441 tttacatatt ttaagtggat tagataatcc tacctctgga caagtgttac ttgatgataa
    10501 agatatatat aaacacactg aaaaagaact atctgcactt agaagaaaat cctttggatt
    10561 cgtctttcaa caatttaatc ttttacctgt tctgacagca tctgaaaata tttcaatgcc
    10621 tgtacttcta gataaaaaac aacctgataa agaatattta aatgaaatct catcattact
    10681 tggaattgca gatagactca atcatcttcc ccatgaactt tcaggtggtc aacaacaaag
    10741 agttgcaatt gctagagcac ttattgcaaa accagatata atatttgctg atgagcctac
    10801 aggaaacttg gacagtaaaa gtggcagtga agttatgaat cttcttataa aaacttcaaa
    10861 acagtttgga aagactttag tggtaattac tcatgatgat agaattgcaa aacttgctga
    10921 tagaaaaatt tcaattattg atggtgttct tatggaggtg aaatagaaat gaagaattac
    10981 ctttcacttt caataaaaga attaaaaagc cagaaactta tgactacatt catcattata
    11041 gcaatagtac tatcaactat tatgacaact gtggttggac agtcaattgg tatcctacaa
    11101 aatttaagaa ttgaacaagc ccgttctttt aacggtgata gacatgtatc ctttcaccaa
    11161 cttactaaaa atcaagttga tgacataaag aaagatgata gagtttatca agctggtact
    11221 tcaataactc ttggcagtag taaaataaag gattctggta tctctgtact tgtaaaagag
    11281 tatgataaaa caggactttc caactacccc aaattaatga aattaaaatc tggaaatttg
    11341 cctaaagata aaaacgaaat agccttagat gaaaacacac taaagcttat gggtataaaa
    11401 ccaagactag gagtgactat acctatgaat ttagatattt cacttttaaa tgataccata
    11461 ccaccatata attacacagc taactttaaa ttgagtggca ttttagagga tgattataca
    11521 ggatatgtaa gtggtatagt aaatggtatt gtaggtaaag gtacatcaga aaatctatta
    11581 cctaagagat atattttatc ctctttggat tttaaaataa aacagcaaga aaagtttcaa
    11641 gaaatagtaa atcaattagc taaaaaaata aatctttctc acaattcaat tcaatataat
    11701 tggatttact taaatgctct taagatacag tttgaaaaag atgaaaatag ttctaatagt
    11761 gatggtatat ctatgattat attggtatct ttatttgttg ccttattggt tttactggcc
    11821 tctggacttg taatctataa tattttaaaa atctctgtta caaagaaaat taaagagtat
    11881 ggatgtttaa gagcaatagg agctgaacca aaccagatat ataagatagt tattttacaa
    11941 atcttaattt tatgtactgt tgccatacct attggagctg taattggaat tatatcttct
    12001 aaaggcataa ctggaatggt cactaatatc ttaaatccag atatacttct agcaaatgat
    12061 aataaagaaa ttactgagct tatacataaa aatacaacag catatatgtt cccattagta
    12121 ttaagcacta atgtatcttt gattttttca tttatttcag cattaccatc agcaatatat
    12181 gcttcacatg tctctcccaa aattgctatg gttggtagta ctaccaaaat caaaagaaaa
    12241 attaaaagag aaaaaacaat aaaaaacttt gaaagacatc tagcatggct aaacttaaaa
    12301 agaaataaag gaagaactat aataactata ctatcattat ttatgagtat aacagtattc
    12361 gttgcactga gtgagttctc taatgtatta gatgtatcta ggtcagtaag taatttaaaa
    12421 gaaggagatt tttccttaac aaatgaaata tctggttttg ataaatcaac actagataaa
    12481 ataactaaaa tgaaaaacgt caatcgcact tcctttataa agtactctga atacaaacag
    12541 ggggaaatag atacagatat aaattttgaa aatagtggtg agatgttaaa aatcattgga
    12601 attgatgagc aaactttaaa agacttaatg ccttctatta cagattctat actagaagac
    12661 tttaaaaatg gttctatctg ttttataaaa aatccgattg ctatttctac accaggtgta
    12721 aaaactagac atacaaattt aaaacctaaa gatagtatca caatcaacaa aaaacaacta
    12781 gatatatata gtacagtaga taaaatgttc tttttacaag gtaatggctg gataaatggt
    12841 gttgatgtta ttgtatatga ttctgtttat aacaccttga caaataaaaa caagttcaac
    12901 cagataaata tttatgctaa agataaatca aaattagaac aaataagatt gtctattgaa
    12961 cagatttgtg aaaataatcc tggctctcat tggatatcat atattgaatc agataaacaa
    13021 cttaaagaga gttttaaaca aatagaattt ctagcatggg cagttatatt atttgtggga
    13081 ttgattggta ctttaaacat cattaacaca actcatacaa atataaatac taggactaat
    13141 gagattggag ttaaaagagc tattggtatg agtaatagta gcttatataa aatgttttta
    13201 tgggaaggtg tttattatgg aatttttgct gctatatttg gttcaattgc tgggtatgcc
    13261 agtgctatta ttataaacat ggcaactata gaaaagcttg atttcactaa tataccaatt
    13321 acttcaattt tacaagctac tattatttct gtacttgcat gtattatagc tactcttatt
    13381 ccgttaagaa aagttaaaaa gatgaatata attgactgta tagatatcaa tttataacta
    13441 ttttacagat aataatataa attaaattat caatgactgt ctctattttc tttcaaatct
    13501 aactaataaa tctttttaac tataatataa gctttaaaaa aggcctatct caaaatagag
    13561 ataagccttt tttcattgtc gaaataatga aaatatattt aacttcacaa aaaaagagtg
    13621 cctcacgaat taaaaatttc gctttgagac actccccttt aatttaaaat atatagttaa
    13681 atgctatgcc aaaatttcac acatgctcca cctgattttg aattttctat tttaacagaa
    13741 cctccaagct gttccactaa ttgtttgaca atgtaaagcc caagaccaga atgtccacct
    13801 tttgtctgcc tagcttcatc tcctcgataa aatttatcaa gagccttttt taaatcttta
    13861 gagctaaacc ctctaccaga atcacaaatc tcataagaaa tacaattttt ttcatccttt
    13921 acagtaatac taatattacc acctgatgga gtgtattgca gactgttgga tatgatatta
    13981 tcaaaaattc gtgtcaacct atccacgtcg atttgaattg gggattgtat attcccctgc
    14041 attttcaata taagttcaac ttccttttga tgagcctgta attcatagtc ttgcaccttt
    14101 tgcctcaaaa attcaggtaa atttatagga acaagattta attgtatatt tgacttttct
    14161 aagtcagacg tatattgcat cttttggaca agagcagcac ttttttctgt attttctctt
    14221 atcactgtaa gatacctatg aagtttttca ttgtcatctg tattattacc aataagtgca
    14281 tctgtatatc ccaaaataat agaaagagga gattttaaat catgtgctag agcttccacc
    14341 atttcaacac gttcctgctc catcttccac tgtgcagata aagagccttt taattcgtct
    14401 ttcatttcag aaaatgcact acaaagttta ccgagttcat tatctgaata atagtcgatt
    14461 tcaaaatcaa ggtccttttc ttttatcttc ttggacgcat caatcaatag atgtaatggt
    14521 tgattgatat tctttacaaa gcgcttagaa agccatctag taaaacccac aatatataga
    14581 aatggagaaa acagagccat tataataact gcaaatacaa atctccctct aatatttgca
    14641 aaggtgattt tcacttggta gaatagaata acagctccct caatctttcc attatcccct
    14701 ttgattggaa cagtatggat ataaccacct ttacgaacag ttttattgat aaaattatta
    14761 aataattctt cttttgtttt aaatagtttt ttggggttag taccatataa aatattgcca
    14821 ttattatcca caacttgata tagcatatca tccccacgaa tcgttctttt taaaccttct
    14881 tcattcgatt gtgataacaa agttatgttt ttttcattga tgtatttttc aattctagga
    14941 acttgtcgct cataatagtt tggtggatag atatccttat ttaaggaatg aatatacaaa
    15001 tagagagcaa atacataagt aatcactgtg gcaactatac tggctattat aataaaaata
    15061 aaagtatgtc taaattgggt aataagggat tgatttttca tttaataacc tctctctctt
    15121 tacattttat tccacttata cccaataccc caaactgtgg aaatatattc tttttctggt
    15181 gtcacagcag aaaacttagc tcgtattttt tttactcgtt caacaacagt tgaggaatca
    15241 ccttcagaat cacaccccca tactttttca tagatttgtt ctcttgagaa aacttgtcct
    15301 gcatggagtg ctagtaattc aacaatatca tactctattt ttgttaaatc aatattctta
    15361 ccatcaattt taattgcacg ttccttcata tataagcata acttcccaaa atacaaagta
    15421 gatctcttgt tctcttcatt gatatactgt gaacgctttt ccctacgcag attagcttca
    15481 atccttgcca taagttcacg cagtccaaat ggctttgtta cataatcatc ccctccaaag
    15541 gtcaaccctt tgattttgtc ggtttctgat tgctttgcac ttaaaaatat aattgggcat
    15601 aaaacatcat ctctaattgc ctgacatacc tcaaatccat tcattttagg catcataata
    15661 tccagtataa taaggtcagg ctcctgtttt gataactcta tacctgcctg accatcataa
    15721 gctatcaaca cttcatgtcc ttttgccttc aattcatcct caagtagcat tactaggtcc
    15781 atttcatcat caataatcaa tattttactc aatataacac cacctttctc ttatgggaag
    15841 gtcagtatta aaacaatatt cgtctactaa taacttttcc ttccctccca cattttaaac
    15901 caaataacgc ctcctatcaa gaatataatc aaacctattg ctactactat aagctctata
    15961 ctagctgttt gcatcacttt agagtaaaat gcctcagttg aaatagttcc agcttcttta
    16021 ataaaataaa ttcctggaag catacctagt tttactggcc atgtccatgg aacaaatgcc
    16081 catacttttt caccaacgct agttaatcca agtattgctg ccatcaaaat tccaccgata
    16141 ctaataccaa tagatgcacc cataccccat gcaaaactca cccaaagatg aatagctaat
    16201 agtggaagtg tcccaatcac cacaagtcct gctgctgaca ggaataacca tattgttgct
    16261 ccagatggca ctgcaatact cattcctatg caaagtatca acgtagcaat aaatgtgcat
    16321 atagttaggc aaaacagaag aaaaagaaat tttcccagat ataatctaac acgagaaata
    16381 cctacacata aaaaaccatt aaaatttcca gcaagctctt cctcctgcac tataaatcct
    16441 gctaaaatac ctactccaat aggaatgatg aatacagtcc atactgtaaa aaatccttca
    16501 aaagcaaatt cttgtgtact tccagattta gttgtgagat aaaaaaccac acataatgaa
    16561 aaaataatag gcatacaaag agtaagccac tggacagctg tcctctttgc tcgaagccat
    16621 tctgatgata atagctttat catttgtcat ccctcctatt gaaccaagct gcagtaagtg
    16681 ctgttaatac tataaacaca accaaagaaa caacaatccc aataggaatc actgaagtat
    16741 tcaaaagtgg gtctccagct tctaatatag taccgttagg atgtatgttg atgattgggc
    16801 ataccattct aactgcccaa ctccaaggta ctgcaatcca aaatggtttt gtcgcagcaa
    16861 ggacaccaaa tatcatacct aaagctccaa ctcccatact taggaacata cctttccatg
    16921 tagctagcca tagctgaagt ggaagtatag caagagaaac aacccagcat acaatacttg
    16981 cccctataat ctgtccaaaa ggaactggac cagctttcaa tattaaacca gttataattg
    17041 ttacgactat taaaactagc gttgaaataa aactgtagac tgccatagct ataactttat
    17101 taatccaaag tgtcatagga gacacattgt gtgtacgtaa agcacgataa tttccagctt
    17161 ttttttcctg gaaagctacc aaagtagcaa acaatgcaaa tccaataggc agaaataaaa
    17221 atgaccacca gttaaaaatt aatgctaaaa gattttgcca cgcccaagaa gttgtttgag
    17281 ataatggatt ttgcatgagt gttgattgca ttataaatgc atatgctgca aaagacactg
    17341 ggaaaaaaac aataagttta cccataaatg ttcttttata ctttaaaagt tctgattgta
    17401 aagcattcat ctgttttgtc ctcctttaag aataacatct gtaaaaaatt cttctagatt
    17461 ttcattaggg tcaggcattc cttgaaataa tagtcgtcct ccatttataa ttcctatatt
    17521 atctacaact tgagcaacct ctgatagaat atggctagaa agaattacag ttataccttt
    17581 ttttggaaaa gaagcaatca attcacgcaa ttcttgaatg ccaaaggggt caagaccatt
    17641 ggttggttca tctagtatta acaactctgg gtcactcaaa agtgcaatag ctattcctaa
    17701 cctttgtttc atacccatag aaaattgtga agctctcttt ttacctgtat ttttcaaatt
    17761 tactatttcc aacacttcat aaattttatc tttaggtatt cccaatattt ttgtatgtac
    17821 taacaaattt tcctctgctg taaggtttcc atacaatgca ggggattcta ttaaagaacc
    17881 tatttttgca agagattctc gtttccaagg ctttctatca aaaataatac gaccatttgt
    17941 tggacgaaga agaccaacaa gcattttaag tgttgtggac tttcctgctc catttggtcc
    18001 taaaagtcca tatatactat ttcgttcaat ttgtagagaa acattgttaa ctgccatttg
    18061 ttctccatat ctttttgata agttttttgt ttcaagaatt aaatctttca tattaatttc
    18121 tcccttcttg taatacctta aatctgttac taagagaata ctaacatgaa aaaataacaa
    18181 ttctataaca cctactaatt ttaataaaat ttaaaataaa aaatagagtt accaatttta
    18241 ttttggcaac tctatttaag tttatgtttt acagttatat tagatttaaa cttagtttca
    18301 ttatcacact aacttcaaat ttactctata atctttaaat caaacactaa tttattaatc
    18361 aaacactaat ctattaattt atatttcttt agacatctaa tatagtttgc tactaaataa
    18421 taattattta tatcatattt atctaatcct ttaaaagaag ttatttcatc attaagctca
    18481 tcactacctt ctacaaaaac acaacctgtt tcaaattcca tacctacaat aatcttacag
    18541 ccactataaa tttcctcttg agtatcgtac aatttttttg catttccttc atttggaatt
    18601 gtaaatacaa taggttttaa ttctttatca ctggaaatct tttgtgaaaa aggcttgttt
    18661 aacttaatat tattgataac tacatgttta aattcctctg aagtttcttc tactattaca
    18721 tattttcgct ttaaaaactc tattatttct aaaacagact tagaatttac ttttaaaata
    18781 cttttatatt tttcatataa agatttccac tgttcaattt tttcctgtgg tatttcttgg
    18841 atatgtgtac ttattttttc ttgagatatt tttttcaatt aaactctcct ttcaatactt
    18901 aaatatcctt atgaatctca catgactaaa gttacaaggt ttctacttaa tagaattttg
    18961 catacttaaa tatgtcttat taattctcta caggatatct ctgtaattcc tacgattctt
    19021 acataagtta cttattttaa attgttatta gttagattct aaaaattatt tattttatca
    19081 ctaatataat actcttaaaa actttacaaa tcaatttaaa tttgctacct tttatttata
    19141 acaacttata gtacatttct cctatgcttt tcaatatatt acttttaaaa ggaggttttc
    19201 attatgattt taaatagagc tgagcttttc cgaggatatt ctttaaatag atttaacgag
    19261 atatgcaata tattatcagt aaataaaatt aaatataagt ataaagtgaa aaataatgct
    19321 tactctaaaa aacctttttt taatgaaata acacttggca atttaggtca aaaggaagat
    19381 ttctcttatg agtacttaat ttatgttcat aaagacgatt atgaacaggc agagtctcta
    19441 ataaattcta agttgcgata gattatttat aaaacatgac attctgttgt catgttttat
    19501 atgttattat actttcaaat atgctactat attttcaaaa ttgtgtgctt taaaactagc
    19561 tttttatagt aatttaaagc ttaaaactaa ggagactcgt tatatgaaaa taaatataaa
    19621 atcaaagtta agtgaattta ttaaacagaa caatcttttt gatgacagca gaataatatc
    19681 ctaccttcct aatataacaa tagaaacaga caaaataaaa actataatga ttaatgaggt
    19741 tgtcccaact aacaccaatg acgattttta tagtgtagat aaagatgctg attatttaaa
    19801 aactactatt cctttatttg acagtgcagg aataaaagta tctaacataa atgatatcct
    19861 agatatgggt atctatataa ctaatgcagt aaaacttcct aaatcagaat atacaataac
    19921 tcgtgataca attaaattac atatgcctat tttagaagaa gaaattaaat tatttaaaaa
    19981 tctagaggta gtaatgttaa tgggagatgt tgcaaaaaaa tcttttaata tgattactaa
    20041 aaagcatata aaaaagaatg tcatcccttc catttcaaca tataaacttc gtaataatga
    20101 actttattat gataatttaa gagtatttcc atcatatatt atgactggtg gaaacatact
    20161 gattgaaaaa tcaaaatttg aaatggcatc agaagatata aaaaaaatgt tcgagattat
    20221 aaacggttga tattacaaaa taataaaaac taatataaaa taaaaagtgc ctcaaaaact
    20281 tctatttatg aggcactttt attttatatt agtgtaacta tatactatta acataattta
    20341 ttatttatat tgttcttttt aatctctatt ccatttagtc taggtaacat tactctttac
    20401 aagattactt tatatttctt ttatatctct aacgttttta tttaataaaa caattattaa
    20461 tacaagtcaa atatatctat ttatctaaaa atatatcttt tacagtaata tctggatggt
    20521 gatacttctt cctattcgtt aaaaaagatt tttttacatt aatagcctct tttggacacc
    20581 agtgaaaaca agctaggcat ctttcacact cattacccca ctttggcttt ttatcaacta
    20641 tacttatatt ctctacagga caaacccttt tacatgtccc acatccatta catttatttt
    20701 ctgcaaaaaa tttagtagtt gcaatttgca tattcttacc tactaaacga agtacaacac
    20761 tatctatatg tgactttaaa ttgttttttc cttctatatt tccaatctcc aagttattga
    20821 tacattttgc aatctcagct accctattag tctgattttt caatctttct tcttcaacat
    20881 ctggaggtgt tatcaaagca tttccaggca tattgattaa aaaaccagca gataattttt
    20941 tccccctttt tttaagtaac ttatctatag taaataaact atgtccagct accccattac
    21001 aagtggcaat ggcatatata tgagtattaa tattaaaatt taatttctct acaaaagact
    21061 ttactatatc aggaacatca gcaaaataaa caggaaaaac aattaccaat atatcttcat
    21121 ttatgtctat attattttct tttctatgta tagctaaggg tataacttct ttacttccat
    21181 ttaacgtttc ttttaaaata tttgaaacta ctagcgaatt tcctgttcca gaaaaataat
    21241 aaatcttact attcattctt atccctcttt ctatatttct acatcatcaa tagttaaaaa
    21301 gtctcttaaa ataggattta tatttacttt ccaattctat ccaaaatatc agtaaacaaa
    21361 ggcaattctt catcagagat agatttaaat gtctctgttg catatatact tgcaatttca
    21421 tttaatggtt cttctaattc tctacctaaa tctgttagga atatttgaaa cttacgtcta
    21481 tctttaacat ctactttacg gacaattaat cccttatttt tcataacatc taagattctt
    21541 gtcgtattag atttatcctt acatgatttt tcacataaat ctatctgaga aattccatct
    21601 tgctcccata gaaaccttaa taacgcataa tggtcagttg ttatatcata tccattttgt
    21661 ttaaacatct tttttaaatc atcttttact tttaacgaag tacttactaa cttagttgta
    21721 gcttcatata gtttctcaaa atctttcatt ttacctccat ttaaataatc ccttgctcct
    21781 cccatctatc aattattccc ttatattgct caatatcttt aaaagccatt ggctttaaaa
    21841 cagcatttat agcaataaaa ttatagattt gagaaagaac aggtggattt ttcttttcct
    21901 ctatgcaata aataaatttt ttagatacct tttgtatctt tgattctact ttttcagtta
    21961 taggattttg attaaatcca gtttttatag ataaactacc acaaacataa cctcccgaat
    22021 ctaagaccat ggattttata aaagaaattg cttttgaacc acgtgcattt tcatatgtag
    22081 taactgctat acatggctta cgatacaata atctttcaag catcatatgt gctctatctg
    22141 taaacacctt gaaaagtcca gttacactac ttccataagt tgggctacca aatataacac
    22201 catcactctt agtaattata tcatgaatat actctacttt atcattttga ttgatacact
    22261 tgcccatttt ataacaattt aaacaaccaa tacaatatga tggatttacc tcacttaaat
    22321 caaaataatt aatttttaca tctggtatta atctttctat atctttatac aagtaagtta
    22381 aaacttttga tgtagctcca ttttttctag ggcttccatt tataactgta attatcattt
    22441 ctatatctcc tttttagttg ttatatcaac aataagtata tcaactatat ttttagctgt
    22501 caatataaac catttattta aagccatact tacacagctt aaataaaagt tttatttaac
    22561 tacatgtgct tatttaatca actataaatg aattattttt aataattgac aaactaaatt
    22621 aatgataata tactttatgt attacaaatg taagtagaag gaggtatagt atgaaaaagt
    22681 taccacatat ttcagaagca gaatatcaag ttatgaaaat tatttggaaa tatgctccta
    22741 tcagcactac tgaagtaatt gaaaagttgg tagaaacaag tacatggagt cctaaaacaa
    22801 ttcaaacaat gttattgaga ttagttaaaa aaggagcttt aacatatgaa aaaaatagca
    22861 gagtattcgt ttatactcca cttgttaaag aagaagaata cgtagctaga gaaagtagtt
    22921 cttttttaaa tcgtttttat aatggtactc ttaattctat ggtactaaac tttttagaaa
    22981 atgataaact atctgaagat gatattgagg aactgagaga aatcttaaat aaaaggacga
    23041 ctaaggggga taaataaata tgtatgcccc attctttata cgatttttac tcagtacatt
    23101 aaccttatca atattaattg ttgtaatttt acttgccaaa aaagtattta aaaaacatat
    23161 ttctgcaaag taccaataca atattggatt tatattgctt ataatgttaa taattccatt
    23221 tgtccctctt aaatatatta atcttggaaa tgtatttaac tatttatata cttttaatca
    23281 acttggaagc agtactaata ggacttctat gataaaaaat agtccaaatt ctgtaattaa
    23341 taactcaaat gcattaaacg atttttcagt atccctaaac caagttaatt ttgatagttt
    23401 aaatatttta ttactaatat catggataat tggaatttta atcatgatag ctattactat
    23461 tcattgcaac attaaaataa aaaatatgaa aaaatcaata caaattttaa gagatgatga
    23521 gatagtattt atatttgaaa agtgtaaaaa agatattggt ataaataagg attttatact
    23581 atgcaaatca cctattataa atacacctat tacatttgga tactttaaac cacacattat
    23641 attaccagat aagtctatat ctaaattatc tttaaaagat ataaaatata tcttacttca
    23701 tgaattacaa cactttaaaa ataaagatat actgataaat tatataatgt gcttccttca
    23761 aatattatat tggtttaatc ctcttgtatg gtatgcattt aaagaaatgc gtatagaccg
    23821 tgaaattgca tgtgatattt cagttttaaa aaagcttgat aaagatagtc atatagaata
    23881 tggacaaact ctaataaact ttgtggataa tatttcaaga ccatcaaatc ttacacttac
    23941 atcagatatt ggtgggtcaa aaaaacaaat aaaaaagcga ctattgaaga taatggattt
    24001 tcataatgaa tcaaatacgt taaaattcaa aagcatttta atttttacat tgatagcttt
    24061 aattgtattt ggaaatattc ccaaactatc tattatggca gatatcaacg aaaaatatag
    24121 acctacaaat gaacaaactg tgtacgagga cttaagtcag tattttaaag gttttgatgg
    24181 aagttttgtt atgtatgatt taaaatccaa gcaatatcat atttacaata aagaaaaaag
    24241 taaaacaaga atttctccaa attctactta taagatttat agtgccttat tgggattgga
    24301 aaatgggata ataacaagaa ataattcaca tatcccttgg gatggaaagc atcattctaa
    24361 tagttcttgg aatgaagacc aagacttatt ttctgctatg gaaaactccg taaactggta
    24421 ctttcagact ctagataaaa aaacagggct aaaaaacata caaacttatc ttaggcaaat
    24481 tggatatggc aattatgatg tttcaggtgg tatctcaaac ttttggctag aatcttcatt
    24541 aaaaatatca cctattgaac aggtagaatt acttgaaagt ttttatacaa ataagtttaa
    24601 atttgaagat aaaaatatag aaacagtaaa agattcattg ttaatatcaa aaaaggatgg
    24661 ggtatcactt tttggtaaaa caggcactgc caatataaat ggaaaagatg taaatggttg
    24721 gtttataggt tatgtggaaa aaaatgaaaa tacttatttc tttgctacaa atattcaaag
    24781 caaagattat tctaatggta gttcagcatc taagataaca ttatccatat taaatgacaa
    24841 aaaaatctat aatccttaat atatttactg tacattctaa aaaattcaca taattttcat
    24901 attaattatc aattaaattc atatacaagg cttattatat aaacatagaa gatagtaaaa
    24961 ctagcttaaa aacataatat aataccctat acaaatagtg gcaactctcc ctcacatcct
    25021 caattgttgc cactatttaa ttttttgtat gaagttaaat atattttcat catttcaaca
    25081 atttgggtgc aatacaatct ctattttgaa acaacctctt taattgtttt agagtttatg
    25141 aattatttct catactttaa aaagattgaa ttactcttga taatcttctt ttgtagtcat
    25201 tacatatcac ctactactca acacatattc tttgacctat ttttacatcc ttgtttatat
    25261 ccaaaatatt taccttctta ccatctggaa acaataccat tgtcaaagta gactttccta
    25321 attcgtgtat tctctttaaa tcaacattaa caagaacatc tccctgttta accatatctc
    25381 cttgtttaac agcaacatca aagccatctc catttaatgc aactgtatca attccaacat
    25441 gaattaatac ttctccatta gttcctttaa tcccaaatgc atgtccactt gaaaccactg
    25501 tttctaattt acctgaaata ggagaaacaa tttttccatc tataatatca atagcaaaac
    25561 catcacctaa cattttttta gaaaatgttt catctggtac attttctaat gcaacaattt
    25621 tccccttagc tggagacaca aagttttcat tattttcatt aaacactaat tcttcgtctt
    25681 catcttcatt tatagcttct ttaggcactc caaataaaca tgttacaata aagcagaatg
    25741 caaccacaaa tattgatact aataagtatg tgattaaatt atatccatca tagatataca
    25801 ttaaataaga aggtaaaact gctaatccat atgaattaga ttgaatatgt aaaattgata
    25861 atattgctgc tcctaaacct gaagatgcac acataataat caaaggcttt aaattatatc
    25921 gaagctgaat cccaaataaa acaggctcac ttataccaaa aagttgagat agcattgctc
    25981 caaccgcagt ttgtttaact tgattatttt tagatttttt cataaatgct agacatgttc
    26041 caatattcgc aaatccatac attgcacaaa gagtaataag ggcattaaat cctgtatttg
    26101 ctaatagtga tgtttcaatc attacataag tatgatgtaa gccagtaata acagctaatg
    26161 gatatgtaaa tccaataata aatcctccaa ttcctaaagg caaatcaatc aataagctga
    26221 tgatatcaac catttttaat tctattgtat gcattatagg gcctacacct aacaaaataa
    26281 ctaacattgt tattagcata acaaaaaatg gtgtgaaaat aaggtctaat acattaggca
    26341 tatgtttacg gaaccatttt tcaagattag ctccaataaa tgctgtaaca atagctgtta
    26401 aaacacttcc ttgacaacct acaataggaa taaatccaaa tgccataatt gcttcaattt
    26461 catttgatgg gtcagcaaca gaatatgcat taggaagtat tggggaaact aacataagtc
    26521 caataacaag tccaataaca ggtgttcctc caaatacttt aaatgctgac catacaataa
    26581 ttgctggtaa aaaagcaaat gctgtttctg taagaactga aacaagtgtc tgaatataca
    26641 atggaatatt tgcagaagac attcctaata atccaagtac attatcatta aacaaacaac
    26701 cctttaatcc caagaataat cctgttgctg caataactgg aataatagga acaaatatat
    26761 ctgctagagt tctcataact ttcttaaatc cagtttcatt atttttaact aattcatctt
    26821 gttctttttt tgataaagtt tttaatccca ttttttcaac ttctgcataa actttattaa
    26881 ctattcctgt tcctaaaata atttgatact gtccagaagt aaagaaaacc cctttaacct
    26941 catcaacttt ttcaactttc ttatcattaa taatttctct atctttaacc atcactctta
    27001 atcttgtggc acaatgtgta atagaaataa tattatcttc accaataatt ttaataagct
    27061 catttgctat tttattatat ttattcattt ttctacctcg ctttcaataa tactgcatga
    27121 aatggtttca aacacactgt tttatttaat agatttatat catctaaatt ttggaataca
    27181 atatcatatt tttcttctac tcttatttct acaggtttat cagaaaaatt ataataacaa
    27241 tcaataacta ccaaatctaa ttgccttcta tacgcaataa catcttcact ttctatatca
    27301 ataggttcta ttgaaccata aatcaaacaa tcagaatact cactatcttg tctcaacttt
    27361 atcatctttt tataaaattc taaaatactc tctgaattgt taatctgact ctgtgcatta
    27421 atatctttat aattttcaat aacctttaac catggtgttg tatctgaaaa acctgcattt
    27481 tctttatctg cccatggaaa tggcgttctt gcattatctc ggcttcttaa attcacaaat
    27541 tctaacgctt cttcttctga gtaaccttca tctattgaac gatagtattg gtcaatgcta
    27601 gataaatcat taaaatcatt tatggtatct cttttaaaat ttgtcattcc taattcctgc
    27661 ccctgataaa taaatggggt tcctcttaaa aagaaataca ttgctcccaa catttttact
    27721 gcatcatcat tcctttcgta ttgcaaaaga tactttgttg tagctcttgg ttggtcatga
    27781 ttttcaataa aattagctgc ccatccatgt tcctgaacat acatttgact atctaaaatg
    27841 cattttctta aatctggaat tgtccatgaa attcttttaa accactcact accagaagca
    27901 acatctaagt cagcatgttt aaagtcaaag accattgaaa agaaaccatc ttctccaata
    27961 aaatcaccta gctggtcata gctaatacct gctgcttcag caacagtcat acaatcatat
    28021 tttttaaacg tattttcttt taattcataa agcatttttt ctattccagg ctgatttcta
    28081 actgtttttg tacatttaac aagaccatca acaccatcac tttctaaact ttgaaaactt
    28141 aaattctttt tgataaatgt aatagcatca actctaaatc ctgccactcc catttgagac
    28201 caggtattaa tcatttggta aagttcactt cttaatattg gattttccca attaaggtca
    28261 ggttgttttt taccaaaaga atgaaaataa tattcgtctc tattctctac cttttcccaa
    28321 acacttccac caaaaacaga cctccagtta tttggtaaat cgtttccttt tttaaaaata
    28381 taataatcat gataaggact ttctggattt tttaatgctt cttgaaacca aggatgttca
    28441 tcacttgtat gattaagaac taaatctaat ataattttga ttcctctatc tttactttct
    28501 ttaattaact cttttaaatc ttccatactt ccaaattcac ttgcaatatc caaataatca
    28561 ctaatatcat acccattatc gtccataggt gatttaaaaa ttggacataa ccacaatgat
    28621 gtaataccta aatcttttaa ataatctaaa cgagaaataa ttccctgtaa atctccaatt
    28681 ccatcattat tactatcttg aaagcttctt ggataaattt gataaacgat ttctttttgc
    28741 caccatttct tttccataaa ttactccttt ttatatgata tttcttcttt ttaattcttt
    28801 ataaattcca ttatcgaata tgtcctcaca tatagatgtc gctatatctt taagttgttg
    28861 atgactattt ttcattgcaa ttgtaacgtc tgatgcttga aacatgacaa tgtcattttg
    28921 accatcacca aaacatatgg tttctttttg agttactcct aatctttctt gtaatctctt
    28981 aattgctttg cccttatgaa aatctttttg aataatttca taatactgac tagaaatatc
    29041 tctctgtgct aattccattt tatcttgtag tatatctttc acttcgtcaa ataccttttc
    29101 atttgaccat aaacaaattt tatgtatatc ttgtgactta tactcttcaa tgttattttc
    29161 atatgttatt ttttcttgga tatgttgttt atttatacaa gaattagttc ctttcaactg
    29221 attcattgtt tcaaatattt ctttggcttt ttgattcatg aaaacttttt cctgactctc
    29281 aatcgaaaaa gcaacttctc tcttttttaa taaacaaaca acttccttta tcaatctttg
    29341 attaaaagat tgattataaa gtaattcacc atgatactga atgtaattac ctcctcctgc
    29401 aatatatcca tcaactccta atgataaaac atcatcttgt attgtaccca tacttcttcc
    29461 agtacaaata acaacactgc aatgattctt ttgacataaa cgtatagcat gctttgcaga
    29521 ttctgggatt ccatatacct catctctaag cgtaccatct atatcaagta ttaataattt
    29581 gtacaatttc atcttccttt cttactatca aaatcataat agcattcaaa aaaaaagata
    29641 tcaatatttt gatatctttt aaaaaatatg ttcattttta gcacaaattt tcattttcgg
    29701 ggaatatgtt cactttctaa atacatcttg tatttaatat aaagcagttc aattaaaata
    29761 aaataaggtg ttgttacatc gtagtccagt tgtttatcta cagaaataga tatagattga
    29821 atatacaggt tttcatcaca taatcttgct aaaggattac ttatcatttt tgtaatagaa
    29881 accatataaa ttcctttcat tctcatttct ttagcaaaat caataattgt agatgcatct
    29941 ccattcaatg aaataaagaa aatcatatca tctgttttta taaaatgatt aagtgcctga
    30001 atcatatcat aaccataaac atcatatata attttccctg ttggtaagaa aatccttttc
    30061 atctctttag caactctttc ctgagaataa ccttctccaa agacgattat acgatttgcc
    30121 tttttcattc tttcaaacat aatcgaacaa tccttcttct ctatatcatc tatcacctta
    30181 ttataacaat ccattaattg gtgtttactt tgatttttat attctacttc tgtttcattt
    30241 aagcgtataa taactcttaa ttcactatat ccaggtaatt gtagtttttg agcaaaacga
    30301 gaaagtgcac ttgtagatat atggtatttg tctgcaaatt catctataga aagtttaata
    30361 caatcttctt tatgattaag aatacactca cagatatact tatcatttgc tgtaaatgaa
    30421 ttataatatt tattgaaaag tccttcaatt ttcattcttt tctccttcta tactctatgt
    30481 aattcatatg aagcaaatct aatagagcat aaaatgctcc tacgacttca taagatatag
    30541 cttgtaatgt atcaagtttt tgcgtttcaa catacagatt atactcacaa agtgttgcaa
    30601 ttgtattatt atcaagccta gttaaagaaa tcgtatgtat atgattgttg ataaattttg
    30661 caatttcaat tcctgctttt gtttctccag acaaagaaat gataataaat aaatcacttt
    30721 tttgaaatga ttctctcata aattcaattt cacctatatc aaataagtca ataacacatt
    30781 tacctactga aagaaatatt ctctttattt cttgagctaa acttttctgt tcattacctg
    30841 tcccataaat ataaattgtt tctgcatcat atattatttt acaaatatct tgaaatgata
    30901 ttttttgtaa ttcattcatt aatagtcgat aattctcgca aacatcatga aaattattat
    30961 cattattaac aaattctagt tgattatttt taagtaaata ctttaaatca gagaatgaat
    31021 ctaatccaat ttttctacaa agccttaata aagtagctct agaaacatta cattcatttg
    31081 caattttttc acacgaaaaa ttacggtatt ctctttgaca aatcaatatt tgtttaataa
    31141 taattttatc attttcattt aaattatcat aattttggtt aactaacgat tctaatctca
    31201 ttttttccac tccctaaata ttattttaat atacattagt tgctttttct agggaagagt
    31261 tttaaataat aaggataaaa taatcatttg attttatttt tattaaaata aaaataacct
    31321 gctaaaagca ggcaccaaaa agtacttatc ctttcgaaca tgtactttag atattgcttt
    31381 cttcagaagc ttcatctctt catactctaa aatagagttt tttctaactc aaaagtgttt
    31441 tccatggtag aaattacttt aattcatctg ccatttactt tttatcttat ttaataatta
    31501 ctattacttg aatatttact tctttaagta aatttcatag ttaacaaagc taa
//
LOCUS       DAELLV010000019        31411 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.019, whole genome shotgun sequence.
ACCESSION   DAELLV010000019 DAELLV010000000
VERSION     DAELLV010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31411
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.019"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            206..1027
                     /gene="yidA"
                     /locus_tag="KPD58_000769"
     CDS             206..1027
                     /gene="yidA"
                     /locus_tag="KPD58_000769"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBG0264553.1"
                     /translation="MYKLIALDIDGTILNTQKRITPEVFESIQEAKRAGAKVVITTGR
                     PLPGVKELLNQLNLTDEGDYVICFNGAIIQEVKSEKIIHDVEMSLDDFDFIYNNVCKK
                     YKTKIHINTMTNLITPNETPGKYTLHEAKLNNIEVKYIQKDKIDESIKICKIMIVDEP
                     ERLEEIIQQLPKNLFNKYTIVRSAPFYLEFLGKTTNKGTALKTLCTNLNIPIENAIAV
                     GDEENDQHMIKYAGLGVAMGNARNSIKEIADYVTDTNNENGVAKVINKYILNKAI"
     gene            complement(1164..1313)
                     /locus_tag="KPD58_000770"
     CDS             complement(1164..1313)
                     /locus_tag="KPD58_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264554.1"
                     /translation="MNFFWTKSDFDAWTNEAGLSNDEDIYCLDINEAIVESYKIFKLK
                     QKVLS"
     gene            complement(1320..1805)
                     /locus_tag="KPD58_000771"
     CDS             complement(1320..1805)
                     /locus_tag="KPD58_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264555.1"
                     /translation="MSQIEKYNSLDNTQQKIRISIMDMIIDKGSSVTLQEVTEYISKK
                     LNIEKEYIERTLQYFIYKNIMVVDGNSINFIYPVSALPTNHKVTLRDNRSFSAMCAID
                     AIGTSCTFNQDIKINSICSATGKEIEVIIKNEKIEYVNNPNLRVLHINLDKHLNWAAS
                     C"
     gene            complement(1818..2570)
                     /locus_tag="KPD58_000772"
     CDS             complement(1818..2570)
                     /locus_tag="KPD58_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0264556.1"
                     /translation="MKLSVRDINKTFVNNRVHTKVLEDISMDIDDGQFVCLLGPSGCG
                     KTTLLTIIGGFQKSERGDVFINDKRVKKPGIDRAFIFQNYALFPWKTIRGNVLFPMKQ
                     QKIPKEKREEMLEELLVMSDLKGKEKLFPHQLSGGMKQRVAMIRALACNPEVLLMDEP
                     LGAVDFQMRQNLQEELERIWIKKKITALMVTHDVDEAVYMSDRVIVMSRDKGRIIDDI
                     NIDIPRPRIRGSQKYEEYKNKLTDTLSKCYEV"
     gene            complement(2570..2707)
                     /locus_tag="KPD58_000773"
     CDS             complement(2570..2707)
                     /locus_tag="KPD58_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG0264557.1"
                     /translation="MVQAQTLMHTDVLIGLMIFAALIGFIIDRVLKFINKILCRWRFA
                     D"
     gene            complement(2747..3376)
                     /locus_tag="KPD58_000774"
     CDS             complement(2747..3376)
                     /locus_tag="KPD58_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0264558.1"
                     /translation="MEIKISKDKSDRLTKKKTDGSMDILYKIILLVIIFLVWHFAAKD
                     IGSSLLLPMPVDVIKGFFFCVTDAETVTNLFITLQRVLKGFMYALLFGLPIGFIMGFS
                     KTFERVLSPVVDSVRQVPIMAWVPLTIVWFGIGDGPTIFLIAFSGVFPIILNTIQGVR
                     AISKDYYNAARSMGASPIVIFTNVIVPASLPDILTGSRIAISTGWMSVI"
     gene            complement(3337..3900)
                     /locus_tag="KPD58_000775"
     CDS             complement(3337..3900)
                     /locus_tag="KPD58_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264559.1"
                     /translation="MQWALSSEEIDIAIICKDAAKQYVNVNSGFEIVGTVVQNSDIFL
                     LSDNNPEKIGVIQNRDYQSELVKKYYKNVEVAPLLGTALAYGLESNLVDGVVIDCIKS
                     IGLEGKRKSTTKLGDYDTYVLVVSKEFKKSKAYSDFITLYNKSVDALKNKATFKKALK
                     EYRNIRNIDMEEISNWKLKFLKIKAID"
     gene            complement(3910..4134)
                     /locus_tag="KPD58_000776"
     CDS             complement(3910..4134)
                     /locus_tag="KPD58_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264560.1"
                     /translation="MENMKNIIILFIIILGSIFIIFFNFEDKEEIKGKLRIGVSDDTS
                     GFVINYMVNRDYFENIKVSDIMDIFTINDC"
     gene            complement(4139..5230)
                     /locus_tag="KPD58_000777"
     CDS             complement(4139..5230)
                     /locus_tag="KPD58_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0264561.1"
                     /translation="MKLSKKIQALILFGVMGTSILTGCSSKPKEKEEAKTGKDDYTIT
                     VGYYNCDHMTAGPVAESAGIYKDLGLNVKTVGNGKVPEAMAAGKMDAGYIGTKGLVGA
                     IPKGSPITIAANNHTGGSEYLIVSKDIKEPKDLIGKKIATDMSDFLWTSDYGPETGLP
                     TDPSKYEVVNMDSDKDKYLALKTGKIQAFTSCDPWGSVAENDGAGKIIASTQYKEKAN
                     GKEYNCCSFSLNKNFIKEHPDLAKKLVLAHTKAIEYIYTNPAEAAKIFAKYYNVEEEV
                     ALRTIYKKTVGEGRTLTWKVTGEEYKNNLQMYKDLKALDDIPKYEDTIDTSLLDSCGA
                     DDFDKFIKEKVNPEFPEGMSYEDWKAKVL"
     gene            complement(5265..5723)
                     /locus_tag="KPD58_000778"
     CDS             complement(5265..5723)
                     /locus_tag="KPD58_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264562.1"
                     /translation="MLKKIVIISCIVVVLIILSKIVDDNIKEDASIPNVNKETLEYFR
                     KNYKEDIITCAEEDLNNDGKKDLVVIYKKSNNSNEMVVVVSDKNSHYITKPIPAPIEN
                     QTITFKNIDDKAPIEVIVSGSKNGNVGYAIYRVEGKKFVDLFGEDMDKCC"
     gene            complement(5716..6801)
                     /locus_tag="KPD58_000779"
     CDS             complement(5716..6801)
                     /locus_tag="KPD58_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBG0264563.1"
                     /translation="MNILEIVLNGVKDTIVASIVTLNGASGWLMISFILAGVLHNVLT
                     PRRFQKQLGNKKFSSLIKSTISGMFLPICSCGVIPLGISMYYSGAYLGPVLAFMTSTP
                     IINPIAVVLCLGLLGPKVTIIYLIAGFLVPFLVGFLGNCFGKEELSLHNDSEEEVIEL
                     EEEKRSFMENMIDGLKWSFGELALTISKYVVMGMVFAGFITTIFPNSIIQKYLGNPGL
                     LSLGSIAILACVMYVCAVGHIPFIAALVASGASPGIAITFLIAGAATNLPELISMFKL
                     IGKRTVIIYSVTLTLSSIIIGYITNLLLVNNNSVNLDATNSSIQLANKLMLNIPDSFE
                     YVCTILIILFAFKSIIPKMKEVIGNHA"
     gene            complement(6828..7013)
                     /locus_tag="KPD58_000780"
     CDS             complement(6828..7013)
                     /locus_tag="KPD58_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264564.1"
                     /translation="MSEKYADKLEIKLYQAGKDFSYIKKYGIITKGTLIINQKKKYDR
                     LNKDTIERAIVEAINNN"
     gene            complement(7175..7507)
                     /locus_tag="KPD58_000781"
     CDS             complement(7175..7507)
                     /locus_tag="KPD58_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HBG0264565.1"
                     /translation="MKVAYIFSSTNTHKILDKMIIPQLEQGTHGVDVLGMFFFMDNTL
                     FLSKGNPVGERLSKLHEKTDMIIMACDQCAIERGIENNLVDGATIGCFPNLYACLGSA
                     GVDQVITL"
     gene            complement(7763..8599)
                     /gene="nadC"
                     /locus_tag="KPD58_000782"
     CDS             complement(7763..8599)
                     /gene="nadC"
                     /locus_tag="KPD58_000782"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HBG0264566.1"
                     /translation="MNYLIIDRMIQDALIEDVPSEDITTNSIVDENSKSTVDLICKQD
                     GIIAGLGVFKRVFEILGDVDVKLYKNDGDRVKNREKIAVLTGSTRNLLVGERVALNYL
                     QRMSGIATITSRYVEKLEGTNTKLLDSRKTIPNLRILDKYSVKVGGGCNHRFNLSDGI
                     LLKDNHIGAAGSVKKAVELARKNTSFVRKIEVEVETLDMVKEAIEANADIIMLDNMSL
                     EMAKQAVDIINGRAIVEFSGNVNLDTIEDIGKIGVDVVSVGALTHSVKALDISMKNLR
                     NI"
     gene            complement(8580..9881)
                     /locus_tag="KPD58_000783"
     CDS             complement(8580..9881)
                     /locus_tag="KPD58_000783"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HBG0264567.1"
                     /translation="MNLEQDVLIVGSGVSGLYCALNLDKSLNVLVVSKSTIENNNTYL
                     AQGGISTARNIDDIESFVEDTMKAGQYKNRVEAVQVLADESIENVGQIVEYGMPLDKE
                     NGEIDYTREGAHSVNRIVHSKDNTGEVVFKTLLKEAKTRENITLIEDAYLLDILKDGN
                     KCIGARIFKSKKEIHVFSKIVVLATGGIGGLFKNSTNQRHLTGDGIAIALRNNIKIEN
                     LEYIQIHPTAFYEENNEGRRMLISESLRGEGAKLLNKNKERFVDELLPRDVVSKAIFE
                     QMEKDKLPYVYLDATHLDSEYLINRFSFIYNECLARGTDITKECIKVSPAQHYFMGGI
                     HVDLDSKTSMENLYAVGEISCTGVHGANRLASNSLLEGLVFSKRAAKNINSVIDNVKV
                     KFIDVPDMDIDIEQVKKENKILVIKEIERTSEDFGDELFDY"
     gene            complement(9894..10808)
                     /gene="nadA"
                     /locus_tag="KPD58_000784"
     CDS             complement(9894..10808)
                     /gene="nadA"
                     /locus_tag="KPD58_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HBG0264568.1"
                     /translation="MDKDLTYQIKELKKEKNAIILAHFYQPPEIQELADAVGDSYYLS
                     EIARDCKEEVVVFCGVRFMGESAKILSPEKTVLMPVSNAGCAMADMVDEEGVIKLKQQ
                     YPNALVVCYINSTAKVKAHCDVSVTSSSAIKILENIDNKEIIFLPDKNLGGYIAEQFP
                     DKNFIFWDGYCKYHNNIRAEEIIELKDKYKNAEVLVHPECKKEIRDLGDYVGSTSGII
                     KYATNSKNKDFIIATEEGILHELKKNNPNKNFYIPGGKILCTDMKKTTLENLYSTLKN
                     MENEVIVEDEIMEKALNSLLNMHKLAEG"
     gene            11226..12683
                     /locus_tag="KPD58_000785"
     CDS             11226..12683
                     /locus_tag="KPD58_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HBG0264569.1"
                     /translation="MTTFLIGLAILLIGGALYGAYCEKVFGPDDRKTPALAQSDGVDY
                     VPMKKWKNSLIELLNIAGTGPILGPIQGILFGPIAFILIPIGCVFGGALHDYMSGMIS
                     IREKGAQMPSLISRFLGNKVFQVYNIFLCLLMLLVGAVFIYTPGDLVVTQILNMKSTI
                     NNPVVWIVYGLIFLYYLCATLFPIDKIIGKVYPIFGAILLLSAAGVGIGIFTQGYDLA
                     NLSLANWKGIHPDGIPLIPTFFVTVACGIVSGFHSTQATLIARSVSNEKEGKTTFYNM
                     MILEGLIAMIWAAAAMGIYNKGIPKELVGSPDVIGLVARDLLGSIGGIIAIIGVIVLP
                     ITSGDTALRSLRLMLADYFHYDQKEKKHRVILSICIFIPVIAILIFAKLSASGFNILW
                     RYFSWSNQTIAIFAFAMITVYLIIKEKNYIISLIPGMFYSFVIFSYIFNAQIGFNLNI
                     NISYVLAAIFTVLYAILTVRSGRKLKSKADTKLAD"
     gene            12906..13334
                     /locus_tag="KPD58_000786"
     CDS             12906..13334
                     /locus_tag="KPD58_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264570.1"
                     /translation="MNNKLHELEKNLPEISCSFCTHLSFKGPSLDYRYDIRCVISDTK
                     PDFRGCCEYFEPEYTELNTGDLDNLYINFLETCLKVNYNDYINSMYWKLFKERTLLEN
                     NFKCSICGSTENVDVYHVNKNLGRETSKDVVVKCDKCNCK"
     gene            13394..13702
                     /locus_tag="KPD58_000787"
     CDS             13394..13702
                     /locus_tag="KPD58_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HBG0264571.1"
                     /translation="MKNNNLNCAATNCAYNTSGYCYAGSIKVDGMQATTTENTYCASF
                     EDKYTSGITSRSNETNQVDTDNIHCEAVKCKYNKNELCKAEKVHINERNASCETFEMK
                     "
     gene            complement(13849..14871)
                     /locus_tag="KPD58_000788"
     CDS             complement(13849..14871)
                     /locus_tag="KPD58_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG0264572.1"
                     /translation="MEDNKGLARDSSKYKIVSISVVVIILITFWYMLNMVLLTFIMTF
                     IFYNLLVATRKRIKKFSSLNIPDSLIIIVLYALFAILLVLISYAVVPIIIVQLTELSR
                     VFSDFDVNQFAQSLGPKLYPIVSKLDFNKYISQAGLLIASTATKVGSFGVNILLAFLL
                     SLLLLLEKNEIKNFGDKLSDSKISFIYNSLVFFGKSFVKNFGEVMKVQVMIAFINSVV
                     SMIFLGFMGFPQIWALGFMIFVLGLIPVAGVIVSLIPLTVIAFNTGGITKVFGVLLMI
                     CIVHAVETYILNPKLMSNRTKLPVCFVFIILLVGEHYLGVWGLLIGVPIFMFLMDILG
                     VKFTSR"
     gene            complement(15046..15552)
                     /locus_tag="KPD58_000789"
     CDS             complement(15046..15552)
                     /locus_tag="KPD58_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HBG0264573.1"
                     /translation="MELWDLYNADGIKTGNVIKRGNSIEEGYYHLAVEVWILNSNSQI
                     LIQKRSKSKKTLPNMWGMTTGCIVSGEDSLEGAIREAKEEIGIDITKDEMKVFRSMIH
                     EDTLWDVYLVKKEYDISKAILQEEEVSDIKWVSTDEIRQLLKEGLFFEYPEIYELLYE
                     IDNNKLNI"
     gene            complement(15612..16313)
                     /locus_tag="KPD58_000790"
     CDS             complement(15612..16313)
                     /locus_tag="KPD58_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264574.1"
                     /translation="MGKISLKSMIRLFSGFFIFAISSVLMINAHVGLMPWDVLHQGLS
                     IKLGITIGQASIMVGVVIVILDAVFGENIGWGTLLNMTFIGIFIDLVIFSGVIPHASN
                     TYIGVFMVVIGIILAAIASFLYLGVCLGSGPRDGLMIALQKKTNKSVRLVRTILEILA
                     LVVGWLLGGSVGIGTLVSALGLGYVLQIVFRIFKFDTKLLKHRFIIDDIREWKEKKSN
                     EHKCKSSIVIKNEQN"
     gene            complement(16531..17601)
                     /gene="buk"
                     /locus_tag="KPD58_000791"
     CDS             complement(16531..17601)
                     /gene="buk"
                     /locus_tag="KPD58_000791"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBG0264575.1"
                     /translation="MTYRILAINPGSTSTKIAVYDGEEQILVKTIDHPAEEIAKYNTI
                     QDQFEMRKEAVLNILKENSIDLKSLSAIVGRGGVLPPVKSGAYLVNEEMIDVLRHRPV
                     LEHASNLGAVVAHAISEPLGINSYIYDSVAVDELIDVARISGLCGMDRSSAGHALNTR
                     AMALKYAKDKGKDYKSLNLIVAHIGGGVSIYLHEKGRMVDMLSDDEGPFSPERSGRVP
                     ATKLVAACYSGQYSEREMTKKIRGKGGIVSYLNTVDAREVEKMIAEGNEEAKIIYEAM
                     AYQLAKGIGELATVVDGKVDAIIITGGIAYSEMFTSMVKKKVEFIAPVEIMAGENELE
                     SLAFGTLRVLNGEEEARIYSEN"
     gene            complement(17654..18229)
                     /locus_tag="KPD58_000792"
     CDS             complement(17654..18229)
                     /locus_tag="KPD58_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HBG0264576.1"
                     /translation="MTKSILLVGVGGQGTILASKLLTMGLMEAGYDVKMSEIHGMSQR
                     GGSVSSQVRYGDCVHSPVIEIGGADILVSFEKMEALRWFNYLKPEGKALVNNHRIDSM
                     TVLIGGAEYQESDIDAELSRLNAKVINAADKAEELGNSKIMNVILLGCLVKSMQLESI
                     DWEKIISENVKPKFVELNIKAFHEGMEMVSK"
     gene            complement(18231..20018)
                     /gene="iorA"
                     /locus_tag="KPD58_000793"
     CDS             complement(18231..20018)
                     /gene="iorA"
                     /locus_tag="KPD58_000793"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HBG0264577.1"
                     /translation="MKQLMTGNEAIARGAYEAGVKYASAYPGTPSTEILENIATYKDA
                     IVAEWAPNEKVALEAAIGGSIAGARTMASMKHVGLNVAADPIFTYAYTGVNGGMVLIT
                     ADEPGMHSSQNEQDNRMYAKFAKIPLFEPSTSQEAKDMIKEAFEVSEKYDTPVLYRVT
                     TRLCHSKGLVECYDREEVEIKEYVKNAKKMVTVPANAQIRRGVVEERMEILKKFSNET
                     DLNYYEINDTKIGVIASGMCCNFAKEVFGKNASYMKLGFTNPLPYEKIKEFAEKVDKI
                     YVIEENDPFIEEQIKAYGIDCIGKDVIPPYGEMTPDVLRKAIFGKTNDTIEYKSELVT
                     PRPPSFCAGCPHRGFFYELGKRKNLIVGGDIGCYTLGFAPPYNGIDYVVCMGSAFGTA
                     HGAQKVLNMKDDNEKRLVGVLGDSTFFHTGINGLLDVVYNRGNSISVILDNRITGMTG
                     HQENPGSGYTLQGAKTKEVDIEGLVKACGIEHVRVINPNNLKEVNEALDWALAIEDEP
                     SVIITRWPCVLKKFSKEDIEEFNNPFKTKCKVDHDKCIGCKLCLKTGCPALSFDKENK
                     LSNIDRNQCVGCGVCAQVCPKQAIVKEEK"
     gene            complement(20079..21338)
                     /locus_tag="KPD58_000794"
     CDS             complement(20079..21338)
                     /locus_tag="KPD58_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG0264578.1"
                     /translation="MSQSMVGKHAMWPKENDVIFSISGRAQAAEKAFGMDNVINATIG
                     ALMDDSGKLITMKTVYEEYKALDNCEIGAYAALEGQPDYLEAVKKVFFRDYLPEGHIR
                     VLASPGGSGAIKLAVWNYTNEGDEVLTSDWFWSPYVSIAEEANRKVVNYQLFDENRRF
                     NFESFKEKFVNIAEKQGRVFTIINTPAHNPTGYSVADDEWDKILDLSKEVAKDKDKKI
                     IFFVDSAYIDFAGDDDVCRKFFKKFSNLPENVLVLVGFSMSKGFTAYGMRMGAIICIS
                     SSEDVAEEFHYSCVHSCRANWSNCNRSAMAVLSNIVNDPKKFKEYEDEKEIYKNMLTR
                     RADVFVKEAERVGLEILPYIAGFFVSIPCDNPKEVCEELTKHNLFAVPLKMGLRFAVC
                     AVSEDKCKKAPSIIKEALESLEVKINN"
     gene            complement(21600..23600)
                     /locus_tag="KPD58_000795"
     CDS             complement(21600..23600)
                     /locus_tag="KPD58_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0264579.1"
                     /translation="MILLRKKIGIIASDIELKERIEELYREDVENGTIIIDILNLDLM
                     ENQGRILVEKGAQAIIGRGGGYSLVIDTVNVPVIPMNMKSTDLLRAIEIAKKYSKKVV
                     LILGDNEVSFDYVGWRNVISTEITEEWFESKYEIRSKVVKYIDQKDEIVIVGGGLACS
                     FARQYGIDSVFATASDESIREAVEYCKKLLDTLGEEKFNNEVLRNILDGIKDGVIAID
                     SNGSIILYNESAKNMLKVERKCALNKYILDVFPKMEWMLDCLHEKEDVEDRKIRNINN
                     LIVNTRTTLIKVDNSTYGVLGIIQDITKLQNLERKIRFDLNQKGLYARYTFDDFLFKD
                     KLTKEFIEEAKKIGKSDYTTLLYGESGSGKEIIAHSIHNISKRKDRPFVAINCATIAE
                     NLLESELFGYEEGAFTGARKGGKRGLFELAHGGTLFLDEINSLSFNIQTKLLRVIEER
                     QIMRIGSDYIIPLDIRIIAATNESLTEKIVMGTFRADLFYRLSSLEINIPPLRDRRED
                     IIPLFNNFVNEVLKDDGLNGINSIDENFVLTKDEMDKLYNYSWPGNVRELKTIAQKYV
                     VTGKIKLRQDRDFKTKKLLPNSEVDKFNSETTASVEVQDESINISKINDGKISIDIKE
                     VNKYVEEKIISMLFAQGLSKNEVAQVLGISRTSLWKKYNKNI"
     gene            complement(23608..25494)
                     /locus_tag="KPD58_000796"
     CDS             complement(23608..25494)
                     /locus_tag="KPD58_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG0264580.1"
                     /translation="MKRSLIEDLKRNINKIEYDMLTKFYLNLTDLNYILDYHKDLLNT
                     KLEDNSRVYTLYLISLINYIKKDREASYNYLIESLKYIKKLSLKDDSDIISKIYIYLT
                     LSAISIRKYDKVNNFYFSAKKIIRQNHLNELLVLLNMSLCVEISALYQTKTDVIALVE
                     EVSFFKTLKNKALVSRANYIFGRVYLYLFNNFIKSMEYLIECLKLAQENNLYSIEAFC
                     KCIISLCYLESGNNVEAIKYINNVLDVAHSNNSISVTERVSIKIDLIWAYLKEDMNKE
                     AEVILLKTIKLMKVVEGVYKDYLYSFIFLYSAEIELKKDNCSFEKVNTYLILSKNIYK
                     SFGDVFIYNSKLDYIYKLYGDLYIKFGNIDEGISCYIKGLNLVKKSSLNLRKISIFYG
                     LIANGYELKKDFKLAIEYYKSMDWYSDRWEKNNSYKTSQAIHKQYELRQKQESLRLLV
                     DDNKKLENDILKDGLTKLYNRRYLEQQLDLFNETRDKNMVAILVDIDCFKSYNDNYGH
                     LAGDKVIVKVTDIIRSVFIDITEHIIRYGGEEILILVDSIESIGVEEIDNIELYIKRV
                     FKLLEFEGIEHIYSKIKNYITISAGVSIKRCRSRKDVEILIEDADKNLYKSKKAGRNQ
                     YIII"
     gene            complement(25521..27362)
                     /locus_tag="KPD58_000797"
     CDS             complement(25521..27362)
                     /locus_tag="KPD58_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG0264581.1"
                     /translation="MEEIDDTILDSMFKLTLNERLLTNNDFIENKIDVVKNLIDCYKT
                     EQNSLMLLKSNFLLALLYGIQGFSEKMKEYILISCKYIDKCLPKDYKFLARFYSQIAI
                     LSLKNEDVNKANLYMDKFNLICDENNFLIEEIIFKSQLIYIKASKCIESSVIIKEIET
                     LYIKIKEINDFNCKKIYFFIIGKIYFLFLDDALIAKVNFLKARKYADLLKDMQVSSLC
                     NIKIGECECSFENYECSMEYFNEVISNKKYRNVNIIQKYRASNNITKILIKTRNYPVA
                     INNLAKSEVYLEKIKNHSLKEVEKFDLFISLAMYYAKSNEKSFEQSICYLDRAKNILN
                     MIKNIEEYIIYDLEIIYHQAKIYYIFGYYDNALNTNKKLLEKSKNAKNYNYVKLAYKS
                     IYLCFEKIQNYEMSMKYFKMYYELKMMYIKARNRNYIDSLNYRHEYIEKEINNMRKVK
                     LDLDKKKYVDHLTSAYNRTFLNDFLEKQEVSEYCTAFMIDVDYFKEYNDTYGHYNGDI
                     ALKNITIIIKKYLKKDMLMIRYGGEEFLILSVCKDYKKSKIFGRKLCRIVKKFLNNNL
                     TVSIGIDTCKNSSIDIKEIIENADKALYKAKESGRNRCLHYHDFKNF"
     gene            complement(27493..27912)
                     /locus_tag="KPD58_000798"
     CDS             complement(27493..27912)
                     /locus_tag="KPD58_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3785 domain-containing protein"
                     /protein_id="HBG0264582.1"
                     /translation="MSLYKFIYDDKEYLLKEENCSALINDEENPVQGVSISKIIDILN
                     EAEEVDFDVEYYQEACPLCLEGVKEKKKFFPFLEYHFYIFSKDGEYVISNISVDYKGL
                     SFNKLSRANKVDNSYIVSVIICENCQDYIVQIENCIV"
     gene            28225..28407
                     /locus_tag="KPD58_000799"
     CDS             28225..28407
                     /locus_tag="KPD58_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264583.1"
                     /translation="MYKDKSDECIHLMTAYIDSISGYYSFIDTQLEDFMMKYGENIVD
                     SNLHSIMMLLCKWGLS"
     gene            28736..29758
                     /locus_tag="KPD58_000800"
     CDS             28736..29758
                     /locus_tag="KPD58_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBG0264584.1"
                     /translation="MDYNDLLKSARENFNGTCKVCKICNGLACAGDVPGMGGKGSGSS
                     FIENRKSLEKIKINMRVIHNVSKPDTSIELFGRKMSSPIFAAPVSGTILNMGGKVSEK
                     EYIEPVVRGCSNSGIYAMVGDTNVDTFLLDNLDVLKDNRGNGIVFIKPWNNSKIIDKI
                     RLSEEAGAFAVGVDLDACGLINNQFQENPFSPKTIDEIRELVESTRLPFIIKGIMTVD
                     DALMAVESGASAIIVSNHGGRVLDYTPGTCEVLPDIAKSVKGKITILVDGGVRTGVDV
                     VKMLGLGADAVLMGRPFVTASFGGGLDGVEFFIEKVRNELCETMILTGCQNVKDIDGR
                     VIWNLL"
     gene            complement(29851..30453)
                     /locus_tag="KPD58_000801"
     CDS             complement(29851..30453)
                     /locus_tag="KPD58_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HBG0264585.1"
                     /translation="MLTKLRKIVAIMCCLLLTFTSVAFADTSKEKGGSKETVPEKNLD
                     SFTLSGYIYTYENAPQEIRDEHKQNCEEINIDPKPDDEIFVPESANLMNEDNSKGVYA
                     SYTVSYNIGAKTITIMSNEYLTISTTKVIRKGNSGKEVKAAQIMLTLLGYNVGIDSSF
                     GSKTYNAVVSFQKKYGLSADGIIGPATWDKLGRLTDPTLS"
     gene            complement(30470..>31411)
                     /locus_tag="KPD58_000802"
     CDS             complement(30470..>31411)
                     /locus_tag="KPD58_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBG0264586.1"
                     /translation="KENALGNNITQIDISTDNNLMLYVAYLWLFVTIVIAVYTFIKYT
                     RFKNLVVDVSYDIEDNDINCIYKNLLKELNIKKKIELRGSDELISPAGMGLFKSYIFL
                     PDYPYSKDELTWILKHELMHFKNKDILIKFLVLSVRIIYWFNPLVYVMSNKVNLDCEL
                     CCDESVLVECSLKDKKEYALALIKSIKFSKNYNSKILTTEFDKTNLEKRLESIVKKKG
                     KSGILIAVLVFMTSITSFVEVDAQVRIDEGNNGMNNNINEFLLNKTTDKIINIYYKDA
                     PNEIRKFYEDKCELEGKIPRDYDIIEMDMKDYNQLIK"
BASE COUNT        10286 a         5410 c         3389 g        12326 t
ORIGIN      
        1 ttattgatta caaagttttt acattctcat ctcacataag taatattttt atcaaaaaat
       61 taatattatt aaactttcta aattacttat ctcttgatat aatagtatac aaaacaaacc
      121 atatttccca ctgaaacttt gttcaacact agctgtgtca acgttttcac aggaaatttg
      181 agtatataat ttattggagg ttgatatgta caaattaatc gcactagaca ttgatggaac
      241 aatattaaat acacaaaaaa ggattactcc tgaagtcttt gagtccattc aggaggcaaa
      301 aagggctggg gcaaaagtag ttataacaac aggcagacct cttcctggtg taaaagaact
      361 tctaaatcaa ctaaatttaa cagatgaagg tgattatgtt atatgcttta atggagcaat
      421 tattcaagaa gttaaaagtg agaaaatcat acatgatgta gaaatgagtt tagatgattt
      481 tgattttata tataataacg tttgtaaaaa atataagact aaaatacaca taaatacaat
      541 gacaaattta attacaccaa atgaaacgcc tggcaaatat acacttcatg aagcgaaatt
      601 aaataatata gaagtcaaat atatacaaaa agataaaatt gatgaatcta ttaaaatctg
      661 taaaattatg atagttgatg aaccagaaag attggaagaa atcatacagc aactacctaa
      721 aaatttattc aataaatata ctatagtaag gtctgctcct ttttatctag aatttttagg
      781 taaaactact aataaaggaa ctgctcttaa aacattatgt acgaatctaa atatacctat
      841 agaaaatgct attgctgttg gcgatgaaga aaatgaccaa catatgataa agtatgcagg
      901 tcttggtgtg gctatgggga atgcacgtaa tagtataaag gaaattgcag attatgttac
      961 tgatactaat aatgaaaatg gagttgcaaa ggttataaat aaatatatac ttaataaggc
     1021 tatctaatca tctaaaaaaa actaaaaata tacctttaga tttaaatact tttcacatcc
     1081 ctactctata ctaatttata tagtatggat attacctatc atttttatct aaaggtcatt
     1141 tttagttttt catatatgta tatctatgat agtacctttt gtttgagttt aaatatttta
     1201 tatgattcta ctattgcctc attaatatct aaacaatata tatcttcatc attagataat
     1261 cccgcttcgt ttgtccaagc atcaaaatca ctcttcgtcc agaagaaatt cattatattt
     1321 cagcaactag cagcccaatt tagatgctta tcaagattta tatgaagcac tcttaagttt
     1381 ggattattca catactcaat cttttcattt tttataatta cttctatttc cttacctgta
     1441 gcactacaaa tagagtttat ttttatatct tggttgaatg tgcaagaggt tcctatagca
     1501 tctatagcac acattgcact gaaacttcta ttatctctca aagttacttt atgatttgtt
     1561 ggcaatgctg atacaggata tataaaattt atactattcc catcaacaac cattatattt
     1621 ttataaataa aatattgtag tgtcctttct atatattctt tttctatatt caacttttta
     1681 cttatatatt cagtcacttc ttgaagtgta acactagaac ctttatctat tatcatatcc
     1741 ataatagaga ttcttatttt ctgttgtgta ttatccaaac tattgtattt ttctatttga
     1801 ctcatgtcta ctcctcttta aacttcataa cactttgata aagtatcagt taatttgttt
     1861 ttgtactctt catatttttg gcttcctcta attctaggtc ttggaatatc tatatttata
     1921 tcatcaataa ttcttccttt atctctcgac attactataa ctctatcact catatataca
     1981 gcttcatcaa catcatgagt taccattaat gcagttattt tcttctttat ccatattcgt
     2041 tctagctctt cctgtaaatt ttgtctcatt tgaaaatcta cagctcctaa tggttcatcc
     2101 attagtaata cctctggatt acatgctaaa gctcttatca tggccactct ttgtttcatt
     2161 cctccagata attgatgcgg aaacagtttt tcttttcctt tcaagtctga cataaccaaa
     2221 agttcttcta gcatttcttc tcttttttct tttggtattt tctgctgttt cataggaaat
     2281 agaacattac ctcttatagt tttccaagga aataaagcat aattttgaaa tataaaagct
     2341 ctatctatgc ccggtttttt aactctttta tcatttataa aaacatctcc acgttctgat
     2401 ttttgaaacc cacctattat agtaagtaaa gttgtttttc cacaacctga tggtcctaaa
     2461 agacagacaa attgtccatc atctatatcc atacttatat cttctaaaac ctttgtatgt
     2521 actctattat ttacaaatgt cttatttata tctcttacac ttaacttcat taatctgcaa
     2581 acctccatct acaaaggatt ttatttataa actttaatac cctatctatt ataaatccta
     2641 ttaaagctgc aaatatcata agcccaatta atacatctgt atgcattaat gtttgagctt
     2701 gtaccattga gtaaccgaga ccggcactcg tagctataaa ctcggctcag ataacagaca
     2761 tccatcctgt acttatagca atccttgaac cagttagtat atctggaaga gatgcgggaa
     2821 caattacatt tgtaaatatc acaattggcg atgctcccat acttctagct gcattataat
     2881 aatcctttga tattgctctt actccttgaa tagtatttaa aattataggg aaaacccctg
     2941 aaaaagctat taaaaatatt gttggaccat ctcctatacc aaaccatact atagtaagag
     3001 gaacccatgc cattattgga acttgcctta cagaatctac tactggagat aaaacccttt
     3061 caaaagtttt agaaaatccc ataataaatc ctattggaag cccaaacaat aatgcataca
     3121 taaaaccttt aagtactctc tgcaatgtta taaaaagatt agtgactgtc tctgcatctg
     3181 tcacacagaa aaagaaacct tttattacat ctactggcat tggaagtaac aaagaactcc
     3241 ctatatcttt tgctgcaaaa tgccagacta aaaatataat aactaatagt attattttat
     3301 ataaaatatc catactacca tcagtttttt tcttagttaa tctatcgctt ttatctttag
     3361 aaattttaat ttccaattgc ttatttcctc catatctata tttcttatat tcctgtactc
     3421 ttttaatgct ttcttaaatg tagctttatt ctttaaagca tcaacacttt tattgtaaag
     3481 tgttataaaa tcactatatg ctttactttt tttaaactct ttacttacaa ctaatacata
     3541 ggtatcataa tctcctaatt tagttgtaga ttttcttttg ccctctaacc caattgactt
     3601 tatgcaatct attacaacac catctactaa attactttct aatccatatg ctagtgcagt
     3661 ccctaataag ggagctactt ccacattctt gtagtatttt ttaaccaatt ctgactggta
     3721 atctctattt tgaatgactc ctatcttttc aggattatta tcacttaata gaaatatatc
     3781 agaattttga actacagtac caacaatttc aaatccacta tttacattta catattgttt
     3841 tgcagcatct ttacatatga ttgctatatc tatttcctct gaactcagag cccactgcat
     3901 tgtacttgct cagcaatcat taattgtaaa aatatccatt atatcactta cttttatatt
     3961 ttcaaagtag tctctattta ccatataatt tattacaaaa ccagaggtat catcagatac
     4021 ccctattctt aattttcctt ttatttcttc tttatcttca aaattaaaaa atattataaa
     4081 tatacttccc aaaataatga taaaaagtat tattatattt ttcatattct ccatttattt
     4141 ataatacctt tgctttccag tcttcataac tcataccttc aggaaactct gggttaactt
     4201 tttcttttat aaatttgtca aaatcatcag ctccacaaga gtccaataag cttgtatcaa
     4261 tcgtatcttc atatttaggt atatcatcta aagcctttaa atctttatac atttgtaaat
     4321 tatttttata ttcttcacct gtaactttcc aagtaagagt tctaccttct ccaacagtct
     4381 tcttatagat agttctaagt gcaacttctt cttctacatt ataatatttt gcaaatattt
     4441 tagctgcttc agctggattt gtatatatat attctattgc ttttgtatgt gctagaacca
     4501 atttttttgc taaatctgga tgttctttta taaagttttt gtttaatgag aatgaacaac
     4561 agttatattc tttgccatta gctttttctt tatattgagt agatgctatt atttttcctg
     4621 caccgtcatt ttcagctaca gaaccccaag ggtcacaaga tgtaaaagct tgaattttcc
     4681 cagttttaag agctaaatat ttatctttgt ctgaatccat atttacaact tcatattttg
     4741 atgggtcagt tggtagacct gtttctggac cataatcact tgtccataag aaatcactca
     4801 tatctgttgc aattttttta cctattaaat ctttaggttc ttttatatct tttgaaacta
     4861 ttaaatattc agaaccacca gtatggttgt tagctgctat tgttattggt gaacccttag
     4921 gtattgctcc aactaacccc ttagttccta tgtaacctgc atccattttt cctgctgcca
     4981 tagcttcagg aactttacca tttccaacag tttttacatt aagacctaaa tctttatata
     5041 ttcctgccga ttctgctact ggtcccgcag tcatatggtc acaattataa taacctactg
     5101 ttattgtata atcatctttt ccagtttttg cttcttcttt ttcttttggt ttactactac
     5161 atcctgttaa tatagatgta cccattacac caaataagat taatgcttga atcttcttag
     5221 ataatttcat ataaatttcc cacctttatg tattattttt atttttagca acatttgtcc
     5281 atatcttcgc caaataaatc aacaaatttt ttaccctcta ctctgtaaat agcatatcct
     5341 acattaccat ttttagaccc tgatacaatt acttctattg gtgctttatc atcaatattt
     5401 ttaaaggtta tagtttgatt ctctataggt gctggtattg gctttgttat atagtggcta
     5461 tttttatcag aaactacgac taccatttca tttgaattat tgcttttttt atatataact
     5521 actaaatctt ttttcccatc attatttaag tcttcttcag cacaagttat tatgtcttct
     5581 ttataatttt ttctaaaata ttctaatgtt tctttattca catttggaat agaagcatct
     5641 tctttgatgt tatcatcaac tatttttgat aaaatgatta acacaactac tatgcaggat
     5701 attataacta tctttttaag catgatttcc tatgacctct ttcatctttg gtattattga
     5761 tttaaatgca aataaaataa ttaaaatagt acatacatac tcaaaactat caggtatatt
     5821 taacattagt ttatttgcca actgtattga agaatttgtt gcatctaaat ttactgagtt
     5881 attattcact aaaagtaaat ttgttatata tccaattatt attgaagaca gtgtcaaagt
     5941 tacagaatat ataataacag ttctttttcc aataagttta aacatactta taagttctgg
     6001 tagatttgta gctgctccag ctattagaaa tgttatcgct ataccaggag atgctccact
     6061 tgcaacaagg gctgctataa acggtatatg tcctacagca catacataca taacacatgc
     6121 aagtattgct atacttccaa gagatagcaa acctggattt cctaaatatt tttgaattat
     6181 actatttgga aaaatagttg ttataaatcc tgcaaaaacc atacccatta ctacatattt
     6241 actaatagta agggctaatt caccaaaaga ccatttaaga ccatcaatca tattttccat
     6301 aaaacttctt ttttcttctt ctaattctat tacttcttcc tctgaatcat tatgaagtga
     6361 taattcttct tttccaaaac aatttcctaa aaatcctact aaaaatggta ctaaaaaacc
     6421 agctattaga taaatgattg taactttagg acctaaaaga cctagacaaa gaactacagc
     6481 tattggattt attattggag ttgatgtcat aaaagcaagt actggtccta aatatgctcc
     6541 tgaataatac atacttattc caagtggtat tactccacaa ctacatattg gtaaaaacat
     6601 tcctgagata gtacttttaa ttaaagaaga aaatttctta tttccaagtt gtttttgaaa
     6661 ccttcttgga gttaaaacat tgtgaagtac acctgccaag ataaaactta tcataagcca
     6721 tcctgatgct ccattcagtg taactataga agctactata gtatctttaa ctccatttaa
     6781 aacaatttct aatatattca tttttgatac tcctaatttt taaactttta attattgttt
     6841 atagcttcta ctattgccct ttcaattgta tctttattta atctatcata tttctttttc
     6901 tgatttatta ttaaagttcc ttttgttata atcccatatt tttttatata cgaaaaatct
     6961 ttccctgctt gatataattt tatttccaat ttatcagcat acttttctga catttttttt
     7021 ataaacactc catgttcatc acaagatggt caagtgttta taaattcaac taaaatttta
     7081 gacatttgtt catcccctac tttaaacaat ttatctttta cttaaaataa tccacagaaa
     7141 ataaattttt ctatggattg tatcctatac taatttataa cgttataact tggtccacac
     7201 ctgcactgcc taaacaagca tataggtttg ggaaacatcc aattgtagct ccatcaacca
     7261 aattattctc tatacctctt tctatagcac attggtcaca agccattatt atcatatcag
     7321 ttttttcatg taatttactt agtctctcgc ctacaggatt acctttagat aaaaataagg
     7381 tattatccat aaagaaaaac atccctagaa cgtctactcc atgagttcct tgttcaagtt
     7441 gtggtattat cattttgtct agaattttat gtgtattagt actactaaaa atataagcta
     7501 ctttcatttt tattccccct atacttttta tattttacaa aattttacag atacaattat
     7561 attataagtt tattcttatt tatctgtaaa atagatatta actatattat taaaagtgta
     7621 tatagtattt atctattttt attaaattat tgattgattt ttttctattt ttattaattt
     7681 taatgaataa aaaatagact ccttaaattt ataaaacaaa tttaaggagt ctattttaat
     7741 taagtttatt tcaatataaa atttatatat ttctcaaatt cttcatactt atatcgagag
     7801 ctttaacaga atgtgtaagc gctcctacag aaacaacatc cacaccaatt tttcctatat
     7861 cttctatagt atctaaattt acatttccgg aaaattctac aatggctctt ccatttataa
     7921 tatctactgc ttgttttgcc atttccaaac tcatattatc caacattatt atatctgcat
     7981 tggcttctat tgcttctttt accatgtcta aagtttccac ttccacttct atctttctaa
     8041 caaaagaagt attttttcta gctaattcaa ctgccttctt aacactacca gcagccccaa
     8101 tatggttgtc tttaagcaat attccatcag ataaattaaa tctatgattg catcctccac
     8161 ctactttgac agaatactta tctaatattc taagattagg tatcgttttt ctactatcta
     8221 ataattttgt atttgtacct tcaagctttt caacatacct acttgtaata gtagctatac
     8281 cactcattct ctgtaaataa ttcaatgcta cacgctctcc tacaagtaag tttctagtac
     8341 tacctgttaa tacagctatc ttttcacgat tctttactct atcaccatca tttttatata
     8401 attttacatc tacatcacca agtatctcaa aaactctctt aaatacacct aagccagcta
     8461 ttataccatc ttgtttgcaa attaaatcta cagtagattt agaattttca tccacaatag
     8521 aattagttgt tatatcttca cttggaacat cttctatcaa agcatcctgt atcattctat
     8581 caataatcaa ataattcatc gccaaaatcc tcacttgttc tttcaatttc ttttatgact
     8641 aatattttat tttctttttt tacctgttct atatctatat ccatatctgg tacatctata
     8701 aacttaactt ttacattatc tataactgag tttatatttt tagctgctct ttttgaaaat
     8761 acaagccctt caagtaatga attacttgcc aatctattgg ctccatgaac acctgtacaa
     8821 ctaatttcac caactgcata taaattttcc atagatgttt ttgaatccaa atctacatgt
     8881 atcccgccca taaaataatg ttgtgcagga gataccttta tacattcttt cgttatgtct
     8941 gttcctctag ctaaacattc attataaata aaagaaaatc tattaattaa atattcacta
     9001 tctaaatgtg ttgcatctaa atacacataa ggtaatttat ctttttccat ttgctcaaaa
     9061 atagcttttg aaactacatc tctaggaaga agttcatcca caaatctttc tttattttta
     9121 tttaaaagtt tagctccctc tcctcttaat gattcagata taagcattct cctaccttca
     9181 ttattttctt cataaaaggc tgtaggatgt atttgaatat attctaaatt ttctattttt
     9241 atattatttc ttaaagctat tgcaattcca tcacctgtaa gatgtctttg attagtagaa
     9301 tttttaaaaa gcccaccaat tccacctgta gctaaaacta ctatttttga aaaaacatga
     9361 atctcttttt tagatttaaa aattctagct cctatacatt tattgccatc ttttaatata
     9421 tctaaaagat atgcatcttc tataagagtt atattttctc tagttttagc ttcttttaat
     9481 aaagttttaa aaacaacttc tccagtatta tccttactat gaactattcg atttacacta
     9541 tgtgctcctt ctcttgtgta atctatctct ccattttctt tgtctaaagg cataccatat
     9601 tctactatct gacctacatt ttctatagat tcatctgcaa gtacttgaac tgcttcaact
     9661 ctatttttat actgcccagc cttcattgta tcttctacaa aggattctat atcatcaata
     9721 tttctagcag ttgaaattcc accttgtgca agatatgtat tgttgttttc aattgttgat
     9781 ttcgatacta ctagcacatt taaactttta tctaaattta atgcacagta taatcctgaa
     9841 actccacttc caactattaa aacatcttgt tctaaattca taagtctatc cctctatcct
     9901 tctgctaatt tgtgcatgtt aagcagtgaa tttaaagcct tttccatgat ttcatcttct
     9961 acaataactt cattttccat atttttaagt gtactatata aattttctaa tgttgtcttt
    10021 ttcatatctg tacatagtat ttttccacca ggaatataaa agtttttatt aggattattt
    10081 ttctttaatt catgtaatat accttcttca gttgctatta taaagtcttt gtttttacta
    10141 ttagtagcat attttattat tcctgatgta ctacctacat aatctcctaa atctcttatt
    10201 tcttttttac attctggatg tactaatact tctgcatttt tatacttatc ttttaactca
    10261 attatttctt cagctcttat attgttatgg tatttacaat atccgtccca aaatataaag
    10321 tttttatctg gaaattgttc tgcaatatat cctcctaaat ttttatctgg aaggaatata
    10381 atttctttat tatctatgtt ttctagtatt ttaattgcac tagaagatgt aacagaaaca
    10441 tcacaatgtg ccttaacttt agctgtagag tttatataac atacaaccaa agcatttgga
    10501 tactgttgct ttaattttat cacgccttct tcatctacca tatctgccat agcacaccca
    10561 gcgttactta ctggcatcaa tacagttttt tcaggtgata atatcttagc actttctccc
    10621 ataaacctta ctccacaaaa tacaaccact tcttctttac aatctctagc aatctcactt
    10681 aaataatatg agtcaccaac tgcatctgct aattcttgta tttctggtgg ttgataaaaa
    10741 tgggcaagta ttattgcatt tttttctttc tttagctctt tgatttgata tgttaaatct
    10801 ttatccataa ttgcaccgct ttctatatgt actagtgtat atacacctgt aaacccatta
    10861 tatcacttga ctattctttc ttcaagaatt ttaaacctct taatattgtt aagatataga
    10921 catttatatt ctcaattgat tttccttttt tctccatcta tttaaactaa tttttatatt
    10981 ttttaatttt tttgtaaaat attatcagaa ttttgtttat attctttatt gctataacat
    11041 tgaaaatatt accaaatacc tgtatactaa gtacatggtt atgtatatgt atttatttct
    11101 gaaattatat tagttgtaat actttataga atttgtaggt tgtccagaat taaattcaat
    11161 ataaatctac acatttagat aatatgtata caaagaccaa aaaattttat aaatggggtg
    11221 atattatgac aacattctta atcggcttgg ctatattact aataggaggt gctttatatg
    11281 gtgcttattg tgaaaaagta tttggcccag atgacagaaa aacacctgca cttgctcaaa
    11341 gtgatggagt agactatgtc ccaatgaaaa aatggaaaaa tagtctaatt gagttgctta
    11401 atatagctgg tacaggacca attctaggac ctatccaagg tatattgttt ggtccaatag
    11461 cattcatact aatacctatc ggctgtgtat ttggaggagc tttgcatgac tatatgagtg
    11521 gaatgattag tataagagaa aaaggagcac aaatgccttc tcttatcagt agatttttag
    11581 gtaataaagt atttcaagtc tataatatat tcctttgtct tttaatgctt ttagttggtg
    11641 cagtttttat ttatacacct ggggatttag ttgttactca aattttaaat atgaaatcga
    11701 ctataaataa tccagttgtc tggatagttt atggacttat ctttctatat tacttgtgtg
    11761 caacactatt tccaattgat aaaatcatag gaaaggtata tccaatattt ggagctattc
    11821 tacttttatc tgctgctggt gttggaattg gaatctttac acaaggttat gatttagcta
    11881 atttaagtct tgcaaattgg aaaggtattc atcctgatgg tattcctctt attcctacat
    11941 tctttgttac agttgcttgt ggtattgttt ctggattcca ctcaactcaa gctactctta
    12001 tagcaagatc agtttcaaat gaaaaagaag gaaaaactac tttttataat atgatgattt
    12061 tagaaggttt aattgcaatg atttgggctg cagctgctat gggtatttac aataaaggaa
    12121 tacctaaaga attagtaggt tctcctgatg taataggttt ggttgcaaga gatttacttg
    12181 gttctattgg tggtatcata gctataatag gtgtaattgt tttaccaata acttctggag
    12241 atacagcttt aagatcttta agattaatgc ttgcagatta ctttcattat gaccaaaagg
    12301 aaaagaaaca tcgtgttata ttgtcaatct gtatatttat tcctgtcata gccatcctta
    12361 tatttgcaaa gcttagtgct tctggattta atatattgtg gagatacttc tcttggagta
    12421 atcaaactat tgccatattt gcatttgcaa tgattacagt ttaccttata ataaaagaaa
    12481 agaattatat tataagctta attcctggta tgttttattc ctttgttata ttcagttata
    12541 tatttaatgc tcaaattgga tttaacttaa acataaatat atcatatgtt ttagctgcca
    12601 tatttactgt attatatgct attttaactg tacgctctgg tagaaagtta aaaagcaaag
    12661 cagatactaa gttggctgac taaatctaaa taaaataata cttagaataa attttattaa
    12721 aaaatgataa aaaagtaggc aatctcaagt tagatttgcc tcttttttat ttaatacagt
    12781 aataaatact taagtattta ctccttattg gaatatttat cctaaatctc taatataaat
    12841 aaataaaagt tttaaaataa tctattcatt tatatactta aaataaattt tttggaggaa
    12901 tacttatgaa taataaatta catgaacttg aaaaaaatct accagaaata tcttgttctt
    12961 tttgcacaca tctttcattt aaggggccta gcctagacta tagatatgat attagatgtg
    13021 ttatttcaga tacaaagccc gattttagag gatgttgtga atattttgaa ccagaatata
    13081 cagaattaaa tacaggagat ttagacaatc tatacataaa ctttcttgag acatgcctca
    13141 aggtaaatta taatgattac atcaattcta tgtattggaa gttatttaaa gaaagaaccc
    13201 tactagaaaa caattttaaa tgttctatat gtggatcaac tgaaaatgtt gatgtgtatc
    13261 atgttaataa aaatttagga agggaaactt ccaaagatgt agttgttaaa tgtgataaat
    13321 gtaattgtaa ataagtgtat aaaatttaga taacttcaca taataagttt aaataaaact
    13381 taggaggtta tttatgaaaa ataataattt aaattgtgct gctactaatt gtgcttataa
    13441 tactagtgga tattgttatg ctggtagtat aaaagtagat ggaatgcaag caactactac
    13501 agaaaatact tattgtgctt cttttgaaga caaatataca tctggtataa ctagtcgttc
    13561 taatgaaact aaccaagttg atacagataa cattcattgt gaagctgtaa aatgtaaata
    13621 caataaaaat gaactttgca aagctgaaaa agttcatata aatgaaagaa atgcaagttg
    13681 tgaaactttt gaaatgaaat aaatttcttt ttatttagtt ttatagtatt tatacaaagc
    13741 aaaattgaac ctatcaaata gatgtcttta ttaatgatgt ctatttatag gttctttttt
    13801 aaaacaattc ataatttata tataaaaata aattattatt ttaatatatt acctagaagt
    13861 aaatttaaca cccagaatat ccataaggaa cataaatatt ggaactccaa ttagtaatcc
    13921 ccatacacct aaataatgct ctcctacaag taaaataata aaaacaaaac aaactggaag
    13981 tttagttcta ttcgacatca acttagggtt taatatgtat gtttctactg catgcactat
    14041 acaaatcata agtaaaactc caaaaacctt tgttattcct cctgtattaa atgctattac
    14101 agttaatgga atcaaagaca ctataactcc tgctactgga ataagaccca acacaaaaat
    14161 cataaatcct aaagcccata tttgagggaa tcccatgaaa cctaagaaaa tcatagaaac
    14221 aacagaattt ataaaggcaa tcataacttg tactttcatt acttcaccaa aatttttaac
    14281 aaagctcttt ccaaaaaata caagcgaatt ataaataaaa gaaatcttac tatcagaaag
    14341 tttatctcca aaatttttaa tttcattttt ttcaagtaaa aggagtaaac tcagtaaaaa
    14401 tgccaataat atatttactc caaaacttcc aacttttgtt gctgttgatg ctataagtag
    14461 ccctgcttgt gaaatatatt tattaaaatc cagctttgag acaataggat acaatttcgg
    14521 tcctaaagat tgagcaaatt gatttacatc aaaatcacta aaaaccctac tcaactctgt
    14581 aagttgaact ataattattg gtacaactgc atagctaatc aatactaaca atatagcaaa
    14641 tagtgcatat aacactataa ttatcaatga atctggaata tttaaagaag aaaacttctt
    14701 aattcgtttt cttgtggcta caagcaagtt atagaaaata aaagtcataa taaatgttaa
    14761 aagcaccata tttaacatat accaaaatgt aattagaata attactacga cagatataga
    14821 aacaatttta tattttgaac tgtcacgtgc caatcctttg ttatcttcca tagtttaaat
    14881 cctccccaaa tttttatgac tttataatga ctgccctttt tattttaatc tcaaaaaaca
    14941 agtagaaaaa tactaaaaat aacttattaa ttttcataaa acaatattaa aatttagaga
    15001 agatacattt taaataatct tacttatact gaaatctgct ctacatcata tatttaattt
    15061 attattatct atctcatata gcaattcgta tatctctgga tattcaaaaa acaaaccctc
    15121 ttttagtaat tgtcttattt catctgtgct tacccacttt atatcactta cttcttcctc
    15181 ctgtaaaata gctttagata tatcatattc ttttttaact aagtatacat cccaaagagt
    15241 atcttcatga atcatacttc taaaaacttt catctcatct ttagttatat ctatccctat
    15301 ttcttccttt gcttcgcgaa ttgctccttc taaactgtct tccccagaaa caatacatcc
    15361 agttgtcatt ccccacatat ttggcaatgt ttttttactt ttagaccttt tctgaattaa
    15421 aatttgagag ttactattta aaatccacac ttcaactgcc aaatggtaat aaccttcttc
    15481 aatagaatta cctcttttta taacatttcc tgttttaatt ccatcagcat tatataaatc
    15541 ccataattcc atttcaaaat ctccttgact ttactataaa ttagataaac tacaaatata
    15601 ttttagcata attaattctg ctcatttttt attacaatac ttgatttaca tttatgttca
    15661 ttgcttttct tttccttcca ttctcttata tcatctataa taaatctatg tttcagcaat
    15721 tttgtgtcaa atttaaatat tctaaacact atttgtaata catatcctaa acctaaagca
    15781 cttacaagcg taccaatacc tacagaacct cctaataacc atccaacaac taatgctagt
    15841 atttcaagaa ttgttctaac aagacgtaca gatttattag tttttttctg aagagcaatc
    15901 ataagcccat ctctcggtcc acttcctaaa caaacaccta agtatagaaa acttgctata
    15961 gctgccaaaa taattcctat aacaaccata aacacaccta tataagtgtt gcttgcatga
    16021 ggtatcactc cagaaaatat tactaaatca ataaaaattc ctataaatgt catgttgagt
    16081 aaagtacccc atcctatatt ttcaccaaaa actgcatcca atataacaat tacaactcct
    16141 accattatag aagcctgacc tatagttatg cctagcttta tagataaacc ttggtgaagt
    16201 acatcccaag gcataagacc tacatgtgca ttaatcataa gcacagaaga aattgcaaat
    16261 ataaaaaaac ctgaaaataa tcttatcata ctttttaaac ttatttttcc catcaaaaat
    16321 cactcctcat aataataata acaataaata ccaataaaat atatatataa atacgaaatt
    16381 ttacagaatt atttattagg ttaagataaa attttaatta taaaaagtgg caatccaaat
    16441 tttatacatt ttcacctata ctctaagctt tcgtgtataa aaaacggata taccactttt
    16501 ttagtatata aacattttta gtttttctta ttaattttca ctataaattc tagcttcttc
    16561 ttctccattt agtactctta aagttccaaa agcaagtgat tccaactcat tttctcctgc
    16621 cataatttct actggtgcta taaactcaac tttcttttta accattgaag taaacatttc
    16681 agaatatgca attccacctg ttataattat agcatctacc tttccatcta ctacagttgc
    16741 taactctcca ataccttttg ctaactgata agccattgct tcataaataa tttttgcttc
    16801 ttcatttcct tctgctatca ttttttcaac ttctctagca tctacagtat ttaggtatga
    16861 aactatacca cctttacctc ttatcttttt agtcatttct ctttctgaat attgacctga
    16921 ataacaggca gccactaatt ttgtagcagg tactcttcct gacctttctg gagaaaatgg
    16981 tccttcatca tcagatagca tatcaaccat tcttcctttt tcatgaagat aaatacttac
    17041 tcctccacca atgtgagcta ctattaagtt taagctctta taatcttttc ctttatcctt
    17101 agcatatttt aaagccattg ctctagtatt taatgcatgc cctgcacttg atctatccat
    17161 tccacaaagt ccagatattc tcgctacatc tataagctca tctactgcaa cagaatcata
    17221 aatatatgag ttgattccaa gaggttctga tattgcatgt gccacaacag cacctaaatt
    17281 ggaagcgtgt tcaagtactg gtctatgtct tagtacatca atcatttctt catttactaa
    17341 atatgctcct gattttactg gtggtaaaac tccacctctt cctactattg cactaagaga
    17401 ttttaagtct atactatttt ctttaagaat attcaaaact gcttccttac gcatttcaaa
    17461 ctggtcttgt atagtattat attttgcaat ctcttcagct ggatggtcta tcgtcttcac
    17521 aagaatttgt tcttctccat catatactgc tatttttgta gaagtagaac ctggatttat
    17581 ggctaatatt ctgtaagtca taattttctt cctttcacct taatttctat agtctattta
    17641 tatctattat aaactatttg ctaaccattt ccataccttc atggaaagct tttatgttta
    17701 attcaacaaa ttttggttta acattttcac ttataatttt ttcccaatca atagactcta
    17761 attgcataga tttaactaga catcctaata aaataacatt cattattttt gaatttccta
    17821 gttcttcagc tttatcagct gcatttataa cttttgcatt taatctactt aactctgcat
    17881 ctatatcact ttcttgatat tcagctcctc cgattaaaac agtcatagaa tctatcctat
    17941 gattatttac taaagctttt ccttctggtt ttaaataatt gaaccatctt aaagcttcca
    18001 ttttttcaaa agatactaat atatctgctc caccaatttc tataactgga gaatgaacac
    18061 aatctccata tctaacttgt gaggaaactg aacctcctct ttgactcatt ccatgtattt
    18121 cactcatctt tacatcatat ccagcttcca tcaatcccat agttaataat ttactagcaa
    18181 gtatagttcc ttgacctcct acacctacca aaagtatact cttagtcata ttatttttcc
    18241 tccttaacta ttgcttgttt tggacaaact tgtgcacata ctccacatcc tacacactga
    18301 tttctatcta tatttgataa cttattctct ttatcaaatg atagagctgg acatccagtt
    18361 ttcaaacata atttacatcc tatacattta tcatggtcaa ctttacactt agtcttaaat
    18421 ggattattaa attcttctat atcttcttta gagaattttt taagtacaca tggccatctt
    18481 gttattatta ctgatggttc atcttctata gctaaagccc aatctagtgc ttcatttact
    18541 tcttttaaat tattagggtt tataactctt acatgttcta ttccacaagc tttaactaat
    18601 ccttctatat caacttcttt agttttagca ccttgtaaag tatatcctga acctgggttt
    18661 tcttggtgac ctgtcattcc agttattcta ttatctaata taaccgaaat agaattacct
    18721 ctattgtaaa caacatctaa aagtccattt attcctgtat ggaagaatgt tgagtctcca
    18781 agtacaccta ccaatctttt ttcattatca tccttcatat taagaacttt ttgagctcca
    18841 tgtgcagttc caaaagcaga acccatacaa acaacataat ctattccatt atatggagga
    18901 gcaaatccaa gagtataaca acctatatct ccaccaacta ttaaattttt tctctttcca
    18961 agttcataga agaatcctct atgtggacat cctgcacaga aacttggtgg tcttggtgtt
    19021 actaattcag atttgtattc tatagtatca tttgtttttc caaatatagc ttttcttaaa
    19081 acatctggtg tcatttctcc atatggtggt attacatctt ttccaataca atctattcca
    19141 tatgctttta tttgttcttc tataaatggg tcattttctt ctataacata tattttatca
    19201 actttttctg caaattcttt tattttttca taaggtaatg gatttgtaaa tcctagtttc
    19261 atatatgatg cattttttcc aaaaacttct ttggcaaaat tacagcacat accagaggct
    19321 ataacaccta tttttgtatc atttatttca tagtagttta aatcagtttc atttgagaat
    19381 tttttaagaa tttccattct ttcttcaaca acgccccttc ttatttgagc atttgctgga
    19441 acagttacca ttttcttagc atttttaaca tactctttaa tttcaacttc ttctctatca
    19501 tagcactcca ctaatccttt tgaatgacaa agtcttgttg taactctata taaaactggt
    19561 gtatcgtatt tttcactaac ttcaaaagct tcttttatca tatctttagc ttcttgacta
    19621 gttgatggct caaataaagg tatctttgca aattttgcat acatcctatt gtcttgctca
    19681 ttttgagatg aatgcattcc cggctcatct gctgtaataa gtaccatacc accatttacc
    19741 cctgtatatg cataagtaaa tattggatca gctgcaacat tcaaaccaac atgtttcata
    19801 gaagccattg ttcttgctcc tgcaattgaa ccccctatag cagcctcaag tgctaccttt
    19861 tcgtttggag cccactctgc aactattgca tctttatatg ttgctatatt ttctaatatt
    19921 tctgtacttg gagtccctgg atatgcagat gcatatttaa caccagcctc ataggctcca
    19981 cgtgcaattg cttcattacc tgtcatcaat tgtttcatat aaaattccct ccatctacaa
    20041 ttattaaatt catagtccac ccaatttgtt caatgaaatt aattatttat ttttacttct
    20101 aaactttcaa gtgcttcttt tataatactt ggtgctttct tacatttatc ttcagaaact
    20161 gcacaaactg caaatcttaa acccattttt aatggaactg caaataaatt atgtttagtc
    20221 aattcttcac atacttcttt tggattatca catggtatac ttacaaagaa acctgctata
    20281 tatggaagaa tttctaatcc tactctttca gcttctttta caaatacatc tgctcttcta
    20341 gtaagcatgt ttttgtatat ttctttttca tcctcatatt ctttgaattt tttagggtca
    20401 tttacaatgt tagataatac tgccatggca cttctattac aatttgacca atttgctcta
    20461 catgaatgaa cacatgagta atggaactct tcagctacat cttcacttga agatatacaa
    20521 attatagccc ccattctcat accataagct gtaaatcctt ttgacatact gaatccaacc
    20581 aatacgagaa cgttttcagg caaattagaa aatttcttaa aaaactttct acatacatca
    20641 tcatcacctg caaagtcaat atatgctgaa tctacgaaaa atattatctt tttgtcttta
    20701 tcttttgcga cttcttttga taagtctaat attttatccc actcatcatc tgcaacacta
    20761 tatccagttg gattatgcgc tggtgtgttt ataattgtaa agactctacc ttgtttttct
    20821 gctatattta caaatttttc tttaaacgac tcaaaattaa accttctatt ttcatcaaaa
    20881 agttggtaat taactacttt tctattagcc tcttcagcta tacttacata tggactccag
    20941 aaccaatcag atgttaatac ctcatctcct tcattagtat agttccatac agctaatttt
    21001 attgcaccac ttcctcctgg tgaagctaat actcttatat gtccttctgg taagtaatct
    21061 ctgaaaaata cttttttaac tgcttctaag taatctggtt gaccttcaag agctgcatat
    21121 gcacctatct cacagttatc taatgcttta tattcttcat atacagtttt cattgttatc
    21181 aattttcctg aatcatccat tagagcgcct attgttgcat ttattacatt atccatacca
    21241 aaagccttct ctgctgcttg agctctgcca gatatgctaa atataacatc attttctttt
    21301 ggccacattg catgcttacc aaccatactt tgactcatac catcacccct aaaattgtat
    21361 ttactcttac attatatatt aagcaattac tatgccaata aatcccactt tatgtaaaat
    21421 tgattttgtt tagaattatt atttttttca ttattaaatt aagtgtttta actatttttc
    21481 ttataaatat atatgcctgt ctaaaaaaat aaaaatttgc aaaaaagtaa tgttaatttt
    21541 tcttaacact tgttttaaaa aattaacatt acttcaaaat aatttatatg taattatatt
    21601 taaatatttt tattgtattt cttccataaa gatgtcctgc ttattccaag aacttgtgca
    21661 acttcatttt tagaaagtcc ttgagcaaaa agcatactta ttattttttc ttcaacatac
    21721 ttatttactt cttttatatc tatacttatc ttaccatcat ttatcttact tatatttata
    21781 gattcatctt ggacttctac acttgctgtc gtttcagaat taaatttgtc tacttcagag
    21841 ttaggaagta attttttagt tttaaaatct ctatcttgtc tcaatttaat tttacctgtc
    21901 acaacatatt tttgagctat tgtctttaac tctcttacat ttccaggcca gctataatta
    21961 tatagcttat ccatttcatc tttagtcaaa acaaaatttt catctatact gtttattcca
    22021 ttgagaccat catcctttaa tacttcattt acaaaattgt taaacaaagg tattatatct
    22081 tctcttctgt ctcttaaagg aggtatattt atctctaaac tactcaatct ataaaataag
    22141 tctgctctaa atgtacccat aactattttt tcggttaaag attcgttcgt tgctgctata
    22201 atccttatat ccagaggtat tatataatca gaaccaattc tcattatttg acgttcctct
    22261 attactctta atagttttgt ctgaatatta aaggatagac tatttatttc atctaaaaaa
    22321 agagtccctc catgagcaag ttcaaatagc cctctttttc caccctttct agcaccagta
    22381 aatgcacctt cttcatagcc aaaaagttca ctttcaagta aattttctgc tatagtagca
    22441 caatttatag caacaaaagg cctatctttt cttttactta tattgtgtat actatgagct
    22501 ataatttctt taccagaccc actttcccca taaagtaaag ttgtataatc actttttcca
    22561 atttttttag cttcttcaat aaattcttta gttaatttat ctttaaataa aaaatcatca
    22621 aaagtatatc tagcatagag acctttttga ttcaagtcaa atcttatttt tctttctaag
    22681 ttttgtagct tggttatatc ttgtattatt cctaaaactc cataagtact attatcaact
    22741 tttatcaatg ttgtcctagt attaacaatt agattattta tatttcttat ctttctatct
    22801 tctacgtcct ctttttcatg taaacaatct aacatccact ccattttagg aaatacatct
    22861 aagatgtatt tattaagtgc acattttctc tctactttaa gcatgttttt tgcactttca
    22921 ttgtacaaaa ttatactgcc attactatct atagcaatta ctccatcttt tataccatca
    22981 agtatatttc gtaacacttc attattaaac ttttcttcac ctaatgtatc taataatttt
    23041 ttacaatatt ctacagcttc tcttatagat tcatcactag cagttgcaaa tacactatca
    23101 attccatatt gccttgcaaa agaacatgcc aaacctcctc ctacgattac aatttcatct
    23161 ttttggtcta tatattttac aactttactt cttatctcat acttagattc aaaccattct
    23221 tcagttattt cagtacttat gacatttctc cagccaacat agtcaaaaga aacttcattg
    23281 tctccaagaa tcaatactac ttttttagaa tatttctttg ctatttctat tgctcttaaa
    23341 aggtcagttg acttcatatt cattggtata acaggtacat ttactgtatc tattactaaa
    23401 ctataacctc cacctctacc aattatagct tgggcacctt tttcaacaag tatacgacct
    23461 tgattttcca ttaagtctag gttaagtatg tcgattatta tagttccatt ttctacatct
    23521 tctctatata attcttctat tctttctttt aattcaatat cagaggctat aattcctatt
    23581 ttttttcgca acaaaatcac ctcaatttta aattataatg tattgatttc tacctgcttt
    23641 ttttgattta tataggttct tatctgcatc ttcaattaat atttcaacat cttttctact
    23701 acgacatctt tttatactta ctccagcact tatagttata tagtttttta ttttagaata
    23761 tatatgttca ataccctcaa attcaagaag tttgaaaact cttttaatat ataattctat
    23821 attatcaatc tcttcaacac cgattgattc tatagaatct actaatatta aaatttcttc
    23881 tcccccatat ctgattatgt gttcggtaat atctataaat acacttctta taatatcagt
    23941 taccttaact ataactttat ctcctgctag atgaccataa ttatcattat aacttttaaa
    24001 acaatctata tcgaccagta ttgcgaccat atttttgtct ctggtttcat taaataaatc
    24061 caactgctgt tccagatatc ttctattata tagtttagta agtccatctt ttaaaatatc
    24121 attttctaat ttcttattat catccactag taatcttaaa ctctcttgtt tttgtctgag
    24181 ttcatattgt ttgtgaattg cttgagatgt tttataggaa ttattttttt cccacctatc
    24241 agaataccaa tccatacttt tataatattc tattgccagt ttaaaatctt tttttagctc
    24301 atatccattt gctattagcc cataaaatat agatattttt ctaagattca atgaactctt
    24361 tttaactaaa tttaaacctt ttatataaca acttatacct tcatctatat taccaaattt
    24421 tatatataaa tctccataca acttatatat gtaatctagt ttagaattat atataaaaac
    24481 atctccaaaa cttttatata tattttttga taaaattaaa taggtgttta ccttttcaaa
    24541 actacaatta tctttcttta actcaatttc agctgaatat aaaaatataa atgaatataa
    24601 ataatcctta tatacacctt caacaacttt cattaatttt atagtcttta ataaaataac
    24661 ttctgcttct ttattcatat cttcttttaa atatgcccat ataaggtcta tttttataga
    24721 cactctttca gttactgata ttgagttatt actgtgtgca acatctaata cattatttat
    24781 gtattttata gcttctacat tatttccact ctctaaataa cacaaactta ttatacattt
    24841 acaaaaagcc tctatagagt ataaattatt ttcttgagcc aattttagac attcaattaa
    24901 gtattccata ctttttataa agttattaaa taaatataga taaactctcc caaatatata
    24961 atttgcccta cttacaagag ctttattttt taaagtttta aaaaaagaca cttcttctac
    25021 taaagctatt acatccgttt tggtctgata taaagcactt atttctacac ataaactcat
    25081 atttaacaat accaacaact catttaaatg attttgtctt ataatttttt ttgctgagaa
    25141 ataaaaatta tttaccttat catattttct tatagaaata gctgataaag taagataaat
    25201 ataaatttta gatattatat ctgaatcatc ttttaatgac aattttttaa tatactttaa
    25261 tgactctatc aaatagttgt atgatgcttc tctatctttt tttatatagt ttataagact
    25321 aattaaatat aaagtataga ctctactatt atcttctaat tttgtattta aaaggtcttt
    25381 gtgataatcc aagatgtagt ttaaatctgt caaatttaga tagaatttag tcaacatatc
    25441 gtattctatc ttgtttatat ttctctttaa atcttctatt aaactccttt tcaagtaaat
    25501 caccaatcct atttcaacag ttaaaaattc ttaaaatcat gataatgcaa acatctattt
    25561 ctaccacttt cctttgcttt atataaagct ttatcagcat tttcaattat ttcttttata
    25621 tctatactac tatttttaca tgtatcaatg ccaatactaa ctgtcaaatt attgtttaaa
    25681 aatttcttta caatcctaca taattttctt ccaaatattt tacttttttt ataatcttta
    25741 catacagata atatcagaaa ttcttctcca ccatacctta tcataagcat gtcttttttt
    25801 agatattttt ttattattat agttatattt ttaagagcaa tatctccatt atagtgtcca
    25861 taagtatcat tgtattcttt aaagtaatct acatctatca taaatgctgt gcaatattca
    25921 ctaacttctt gtttttctaa aaaatcgttc aaaaaagttc tattatatgc acttgtcaaa
    25981 tggtcaacat actttttttt atctaaatca agttttactt ttctcatgtt atttatttct
    26041 ttttctatat actcatgtct ataatttaag gaatctatat aatttctatt tcgagctttt
    26101 atatacatca tttttaactc ataatacatt ttaaagtatt tcatactcat ttcataattt
    26161 tgaatctttt caaaacacaa atatatactc ttgtatgcta acttcacata attataattt
    26221 ttagcatttt tagatttttc aaggagtttt ttatttgtgt ttaaggcatt atcataatat
    26281 ccaaagatat aatatatctt tgcctggtga taaattattt ctaaatcata tattatatat
    26341 tcttctatat tttttatcat atttaatatg tttttagctc tatctaaata gcatattgat
    26401 tgttcaaaag atttttcatt actttttgca taatacatgg ctaaacttat aaataaatca
    26461 aacttctcta cttcttttaa gctatgattt ttaatttttt ctagatatac ttcagatttt
    26521 gctaaattat ttattgcgac aggataattt ctagtcttta ttaatatttt agttatgttg
    26581 tttgatgctc tatatttttg aattatattt acatttctat actttttatt gcttattact
    26641 tcattgaaat attccatgga acattcataa ttttcaaacg aacattcaca ctcacctatc
    26701 tttatattac atagtgaaga tacttgcata tcctttagta aatctgcata ttttcttgct
    26761 tttaaaaaat ttaccttagc aattagagca tcatccaaaa acaaaaaata tattttacca
    26821 ataataaaaa aatatatttt tttacaatta aaatcgttaa tttcttttat ctttatatac
    26881 aacgtttcta tctctttaat tattacagaa gattctatac acttacttgc cttaatgtaa
    26941 ataagttgtg atttaaaaat aatttcttca attagaaaat tattttcatc acaaataaga
    27001 ttaaatttat ccatatataa atttgcctta tttacatctt cattttttaa acttaaaata
    27061 gctatttgtg aataaaatcg tgctaaaaat ttataatctt tgggtaagca tttatcaata
    27121 tatttacagg aaatcaaaat atactctttc attttttcac taaacccttg aataccatat
    27181 aataaagcta gcaaaaaatt tgattttaat aacattaaac tattttgttc agttttatag
    27241 caatcaatta aattctttac tacatcaatt ttattttcaa taaaatcgtt attagtcaat
    27301 aatctttcat tcaaggttag tttaaacatt gaatctaaaa ttgtatcatc tatctcttcc
    27361 aaatatttac tatatattgt aactcacctc cgacttacat tatctcataa atttagtaat
    27421 tttccatact atttttgaaa aaagttgatg tctcaattta gaaatttctc ttaaattaag
    27481 acatacttta cactaaacta tacaattttc tatctgaact atataatcct gacaattttc
    27541 gcaaattatc acacttacaa tataactatt atcaacttta tttgcccttg atagtttatt
    27601 aaaagatagt cctttataat caacagatat attgcttatt acatattctc catctttact
    27661 gaaaatataa aagtgatatt ctaaaaaagg aaagaatttt ttcttttctt ttactccttc
    27721 taaacataat ggacatgctt cttgataata ttctacatca aaatcaactt cttctgcttc
    27781 attaagtata tcaattattt ttgaaatact tactccttga actggatttt cttcatcatt
    27841 tattaaagca ctacagttct cctcttttaa taaatattcc ttatcatcat atataaactt
    27901 atataaactc atattatatc ctccttataa ttattaatct ccaagttatt ctaccattta
    27961 aataaagtta ataaaagtaa ttgtttataa tctttataat taagttatgt atacatatat
    28021 tcctatgcgc tatcttaatt aatgatttta aaatccaagt ttgcactttg aacatccagt
    28081 ttcccaaaat ctattgtcaa aactcacttt agttattaat ataatgatat aaatatataa
    28141 ctgcatagat gttacacact taactattta atatgatacg atttggtatc taaatttata
    28201 tattgtatat agggggagat atatatgtat aaagataaaa gtgatgaatg tatacactta
    28261 atgactgcat acatcgatag tataagtgga tattattcat ttattgacac acaattagag
    28321 gattttatga tgaaatatgg tgaaaacatt gttgattcaa atttacacag tataatgatg
    28381 ttgctttgta aatggggatt atcttagcat ttcaagaaac ttttttgtat attgtattct
    28441 atattattta ttcattaaca aaaaagattg acataaactt aaaagctata tatagatttc
    28501 tttaaaaatc tatatatagc ttttttaatt ttttcaaaat gtatacttaa ataagttagt
    28561 ttttttcaaa atttgactat ctaatatgat ataatttaac tatctacaac aataaaaaat
    28621 atctaatatg tattatctag tttagctcat cataataaca tccctatatt tacaattaag
    28681 atattttcaa tatagtaata ctcaatttta atattataaa aaggagagat tttgtatgga
    28741 ttacaatgac ttactaaaat cagctagaga gaactttaat ggaacatgta aagtatgtaa
    28801 aatttgtaat ggattagctt gtgctggaga tgttcctggt atgggtggta aaggaagtgg
    28861 ttcgtctttc attgaaaata gaaaaagtct agaaaaaata aagataaata tgagagtcat
    28921 acataatgtt tcaaagccag atacatccat agaattattt ggtagaaaaa tgagttctcc
    28981 tatattcgct gcacctgtat caggtactat attgaacatg ggtggtaaag tttccgaaaa
    29041 agaatacata gagcctgttg ttagaggttg ttctaattct ggaatttatg ctatggttgg
    29101 cgatactaat gtagatactt ttttactgga taatttagat gtattgaagg ataatagggg
    29161 aaatggaatt gtatttataa agccttggaa taattcaaag ataatagata aaattagatt
    29221 gtctgaagaa gcaggtgctt ttgcagttgg tgtagacctt gatgcttgtg gacttattaa
    29281 taatcaattt caagaaaatc ctttttctcc taaaacaatt gatgagatta gagaattggt
    29341 tgaatccact agattacctt ttattataaa aggaattatg actgtagatg atgctttgat
    29401 ggctgtagag tctggtgcta gtgccattat tgtttcaaat catggtggaa gagtacttga
    29461 ttatactcct ggtacttgtg aggtacttcc agatatagct aagtctgtaa agggaaagat
    29521 aacaatactt gttgatggtg gagttaggac tggtgttgat gtagttaaga tgcttggatt
    29581 gggtgctgat gctgttttga tgggaagacc ttttgttact gcttcttttg gcggtggtct
    29641 tgatggtgtg gagtttttta tagagaaggt taggaatgag ttatgtgaga ctatgatttt
    29701 gactggttgt caaaatgtta aggatataga tggtagagtt atatggaatt tattgtaaaa
    29761 tagttttata acatttatat ttaaatagtg tctcatttaa attgaaaaat catattgaga
    29821 cactatttaa tatattttta gattttaatc ctatgaaaga gtagggtcag ttagtctacc
    29881 caatttatcc caagtagctg gaccaattat tccatctgca gacaaaccgt attttttttg
    29941 gaaacttaca actgcattat atgtcttact tccaaaagaa ctatctatac ctacattata
    30001 accaagtaaa gttaacatga tttgtgcagc ttttacttct tttccgctat taccctttcg
    30061 tataactttt gttgtactta tagtcaaata ctcattactc ataattgtta tagttttagc
    30121 acctatatta taagaaacag tataacttgc ataaacacct ttactattgt cctcattcat
    30181 tagattagct gattctggaa caaatatctc atcatctggc ttagggtcta tatttatctc
    30241 ttcacaattt tgtttatgtt catctcttat ttcttgtggc gcattttcat atgtatatat
    30301 atacccagaa agagtaaaag aatctaggtt tttctctgga acagtctctt tacttcctcc
    30361 tttctcttta cttgtgtcag caaatgccac acttgtaaaa gtaagcaata agcaacacat
    30421 aatagctact atttttctta attttgttaa cataaaatcc tccttataat tattttatta
    30481 attgattata atctttcata tccatttcta ttatgtcata gtctctaggt attttacctt
    30541 ctaattcgca tttatcttca taaaatttcc taatttcatt tggtgcatct ttataataaa
    30601 tatttataat tttatcagtc gtcttattca ataaaaattc attaatattg ttattcattc
    30661 cattattacc ctcatcaatt ctaacttgag catcaacttc aacaaaagat gttatagaag
    30721 tcataaatac taaaacagca attagtattc cacttttgcc tttcttttta acaatactct
    30781 caagtctctt ttccaaattt gtcttatcaa actcggttgt caaaatctta ctattataat
    30841 ttttactaaa cttaattgac tttataagtg ctagtgcata ttctttttta tcttttaaag
    30901 aacattctac caaaacactt tcatcacaac ataattcaca atctagattt actttattgc
    30961 tcattacata tacaagtgga ttaaaccaat atattattct aacagacaaa actaaaaact
    31021 ttattaaaat atctttattt ttaaaatgca ttaattcatg tttcaaaatc caagttagtt
    31081 catctttact ataaggatag tctggtaaga atatgtaaga tttaaataat cccatacctg
    31141 ctggacttat caattcatca gaacctctta attcaatttt cttttttata tttaattctt
    31201 ttaacaagtt tttgtagata cagtttatat cattatcttc aatatcataa gatacatcaa
    31261 caactagatt tttaaatctt gtatacttta taaatgtata aactgcaatg actatagtta
    31321 caaataacca taaataagct acatacagca taagattatt atctgtagaa atatcaattt
    31381 gtgtaatatt atttcctaat gcattttctt t
//
LOCUS       DAELLV010000020        30943 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.020, whole genome shotgun sequence.
ACCESSION   DAELLV010000020 DAELLV010000000
VERSION     DAELLV010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30943
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.020"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(255..1592)
                     /gene="xylA"
                     /locus_tag="KPD58_000803"
     CDS             complement(255..1592)
                     /gene="xylA"
                     /locus_tag="KPD58_000803"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HBG0264587.1"
                     /translation="MSEIFKGIGQIKFEGVKSDNELAFRYYNPEQVVGNKTMKEHLRF
                     AMSYWHTLCGEGNDPFGVGTVERPWNNITDPIEIAKIKVDAGFEFMSKMGIEYFCFHD
                     RDIAPEGRDLEETNKILDEIVEYIKVNMEKTGIKLLWGTANMFGNPRFVHGASTTCNA
                     DVYAYAAAQVKKAMEITKYLGGENFVFWGGREGYETLLNTNTELEMDNFARFLQMAVD
                     YAKEIGFSGQFLIEPKPKEPTKHQYDFDTATVLGFLRKYNLDKYFKMNIEANHATLAG
                     HTFQHELNIARINNVLGSIDANQGDLLLGWDTDQFPTNIYDATLAMYEVLKQGGIAPG
                     GFNFDSKVRRASFEVEDLFLAYIAGMDTFAKGLLIAHKLLEDEVFENFTKERYASFSE
                     GIGKDIVEGKVGFKELESYALQMPVIKNKSGRQEMLEAILNRYIYEVDTISNK"
     gene            complement(1688..3238)
                     /gene="xylB"
                     /locus_tag="KPD58_000804"
     CDS             complement(1688..3238)
                     /gene="xylB"
                     /locus_tag="KPD58_000804"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HBG0264588.1"
                     /translation="MKLEYVLGVDIGTSGTKTVLFDKLGNTIKSCTYEYPLIQEKSGW
                     AEQDANDWWKAVVESIREVVQSSNISSECIKGIGLSGQMHGLVILDNEGKTLRNSIIW
                     CDQRTVKECEEITDLVGEERLIEITANPALTGFTASKILWVRNNEPDIYKNTNKILLP
                     KDYIRYKLTGEYATEVSDASGMQLLDIRKRDWSDEVLEKLNIDKNLLGKVYESQEITG
                     YVTRDVASLTGLKEGTIVVGGAGDQAAGAIGNGIVKDGVVSSTIGTSGVVFAYTKEPK
                     IDKEGRVHTFCHAIPNTWHVMGVTQGAGLSLKWFKDNFCQSEVEVSNSLGEDVYEIIN
                     NQVSKVPTGCNGLLYLPYMMGERTPHLDPYARGVFFGLSPIHSKKEIARSIMEGVSYS
                     LKDCMDIIENLNIDVNEVRASGGGGKSKVWRQMQADMFNQDVYTINSSEGPALGVAIL
                     ALVGAGIYENIQKACDAIIKTSTKLEPISENVDTYNIYHRLYKRIYKSLKDDFKLLDE
                     VVNAKINN"
     gene            3446..4606
                     /locus_tag="KPD58_000805"
     CDS             3446..4606
                     /locus_tag="KPD58_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HBG0264589.1"
                     /translation="MVTDKYTIREMNERLVLEQIIKNGPISRASIASTIGLNKATISA
                     ITKKLIDESLVHEIGIGNSTHSGGRKPILLVFNKCAGISLSMDIGYDYIFSSLSYLDG
                     TIINSKKLTDIQVSKDNVIQLIDEIINSYNISKIDTPYKVIGLTLAIHGITCENKVLF
                     TPYYNLNEIDLYSILSKKYDFPIHIENEANLTALAENTFSTVHNSLLSLSIHSGFGSG
                     IIINNKLYSGRNGMSGEIGHTIIMPNGKLCPCGNRGCLEQYCSEKKVFEQLSSLENIP
                     KIDSDIVKQLYYEDNQNAKKVIHEFCSYLTIAINNAITTYAPEIIYLNSQIISDIPEI
                     LQITKDMLVSSFNKGINIEISSLGSEASLYGGSAVNIKSFLNIQNLTLINEI"
     gene            complement(4762..5166)
                     /locus_tag="KPD58_000806"
     CDS             complement(4762..5166)
                     /locus_tag="KPD58_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0264590.1"
                     /translation="MNSIILMSHGNMAEEVLNSAKMIIGDSIDYPTVNMKQDDGIEGT
                     IEKLKKALEVYKESSEIIIMADLLGGTPCNAALLVANNDSRIKIISGLNLGMVIESAF
                     LDTKELAAKLEKIGKDNISMVEQNASLLSDDE"
     gene            complement(5187..5669)
                     /locus_tag="KPD58_000807"
     CDS             complement(5187..5669)
                     /locus_tag="KPD58_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0264591.1"
                     /translation="MEMKGIKNIRIDDRLIHGQVATMWSNKLGVTRLMVVNDAVANNS
                     VQKQVLRMATPAGIASSIITEETAIKNITAGKYEGQNVLLIVKSPVDLIPFVEAGFKI
                     HNINVGNMSSRKDTTVLRPNISVTEEEKEAFKRLLDEGIEITTIMTPDDKKTYLVDIL
                     "
     gene            complement(5689..6498)
                     /locus_tag="KPD58_000808"
     CDS             complement(5689..6498)
                     /locus_tag="KPD58_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG0264592.1"
                     /translation="MSDKVSLNKSNEKVKKSVLWRWFFTSSVSSNYEKMQALAYCYAV
                     LPFLKVVYKDNPEKLQSAVLNHLQFFNTNPWVAPYILGINVAMEELSSEETEEAVASI
                     KTGLMGPVAGLGDSLFVVIPWTIFGAIAANMAIDGSPFGILLWIVVSVALKLLSIPLF
                     NAGYTSGTKLISSIEKSLKILTESTSILGLMVVGALIPSVVKANVALNFQQGDFTMKG
                     QEILDQIMPGLVPAILVVIVYWALKKNIKPIYLILGVMVVSIILYALGILK"
     gene            complement(6491..7270)
                     /locus_tag="KPD58_000809"
     CDS             complement(6491..7270)
                     /locus_tag="KPD58_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0264593.1"
                     /translation="MEPQLWQLLLIVLYGFFINYEKNSTMFGTYQPVTAGFITGLILG
                     DINTGLYIGGTLQLLSLGISNFGGASIPDYQTASIVATFITITTKQEASVGISIGIPV
                     ALLMVQLDVLRNTIGIWLVHKAEDGAKKGNYKNITYMQMLGVLLTAATTGIPVALSVI
                     FGPSLINTILKYTPEWLTGGLTVAGGLLPAVGIGLLLRYLPAKEYFSYLVIGFVLAVY
                     MKVPLLGVALIGGAIALIIYKKNLENQEQQYTVVGGMDEDE"
     gene            complement(7270..9348)
                     /locus_tag="KPD58_000810"
     CDS             complement(7270..9348)
                     /locus_tag="KPD58_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBG0264594.1"
                     /translation="MIKISEDYIEKRFDNELLRIEAWGKNSLRIRSFVDQNFVDENYA
                     LNEKPKLNKDDITINKNEDGSAIIKNGKIKAVLDHRDRITFYNDKNEILLKEYIRLRA
                     VKHDDGGEDVGTIEITKDFNSTLKLKSREYRPSYSGEFEVTTRFESEPSEKIFGMGQY
                     QHKFLDLKNTVLELAQRNSQISVPFYISSLGYGLLWNNPGIGKVSFAKNMTEWKMFST
                     NFIDYWITCGESPKELNKNYSQVTGTVPMMPENLLGLWQSKLRYRTSEEVLDVVKEYS
                     KRGIKLSSIAIDYFHWPKQGEYKFDLDYWKNPKELVKKLKEEYSVEPIVSVWPTVQSD
                     AENYNDYLENGYLVNVNRGVRMTMQIQGNTVFVDMTNENAREYVWDRIDKNYKQLGID
                     YYWLDVAEPGYSVYDFDNYRYKKGNVLSCGNIYPIDYLKMIYDGLHNDIESVVTLVRG
                     AWAGAQKYGALVWSGDIDSSFEAFNNQVNTGLNMGLAGIPWWTTDIGGFHGGNPKDPE
                     FRELMVRWFQYATFSPILRMHGDRLPHSKPLSNKGGGSMVTGAPNEIWSYGEEVEVIL
                     TKFIKIRESLKTYLKKLMKEAHEDGTPVMRTLFYEFPEDDKTWEVDNTYMLGDEILVA
                     PIMNYKDRSRKVYLPKGHTWENIFSGVSYEGGKTYEVECPLEEIPIFLKQDSSYNFKE
                     LKNILGEVKS"
     gene            complement(10147..10668)
                     /locus_tag="KPD58_000811"
     CDS             complement(10147..10668)
                     /locus_tag="KPD58_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBG0264595.1"
                     /translation="MKRNREIVVVCHCILNCNSKVEGLSEFKGAQNIAIDLINKGYGI
                     IQLPCPEMIMYGINRWGHVKEQFDNLFFREQCRQMLIPHVRQFDEYLKNDYDIKSIIA
                     IDGSPSCGYNKTCSSKRWFGELSGCNELMDKINDIQMVDGKGIFIEELEKLLKEYNLE
                     IPIVGFDELREDK"
     gene            10834..11400
                     /locus_tag="KPD58_000812"
     CDS             10834..11400
                     /locus_tag="KPD58_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG0264596.1"
                     /translation="MDTKKVTFSSMCIVINIVLGMVISMINIPLLFLDTVGTVLGAVV
                     LGPFWGALIGGCTNLVLGVISGPTNIPFALVNIVLGLIVGYISKKKGFGYKEAIITGV
                     ILSIVCPLIGTPISVLLFGGLSGSGADLLVGFLVQSGQKIFTAAFFPRILSNIVDKPL
                     SCIMVVYFISKMPKGFIKQYSKNTSSSY"
     gene            complement(11617..13680)
                     /locus_tag="KPD58_000813"
     CDS             complement(11617..13680)
                     /locus_tag="KPD58_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HBG0264597.1"
                     /translation="MKAGEIEFLSYLEGSNKSFVVPVYQRNYNWKKEQCKRLFDDLED
                     IIDSGFRTHFLGSVVSIYSMGKEYLIIDGQQRVTTVSILLIAMCQIIKEFGESKGIIT
                     TEEQIKECYLINKHSRDKDGKIKLKPIAEDRKAYYELFEEEMNTDRNSNIVSNYLYFY
                     NRIKEDNISIDDLFDAIQKLTIVEIELKNGEDDPQLIFESLNSTGLDLSEADRVRNFV
                     LMGRSSKLQEEFYNNYWYKIEKNTSSNVSSFIRDYLTIKERSIPNKNKVYFSFKRYVS
                     DNGIDIEILLKDLLKFSNYYREISYSCTDDNDVNKVLKRINKLEIFVSYPFILELFDD
                     YNEGIINKKVLVESLNLVECFILRRIVCDVPPNALNKVFMNLGREIKKFSDYKENYSN
                     ILRYIFINKKSSQRFPTDSEFSNAFETKDIYNTKSKNKLYILERLENYNNKEIVDLEN
                     LINNNMLNIEHIMPQTLTNKWKESLGDNYKEIHDKYLHTIGNLTLTGYNSKLSNKTFE
                     EKKEMEDGFKDSRLYLNKYISSIDKWNEEEIKNRTKILLDRAVEIWKYPSVTYEPPSN
                     SENIFTLEDDDVNFTNESIVSFSILGESYKAKNWTEFYEQVALTLYDLDSVRFNSIVD
                     KTYKRDFIDNIKSRDAYKLRKGIKISENIYLETNLNTEAKLNIIRFLLNEFDIELNEV
                     IFSIK"
     gene            complement(14061..17396)
                     /locus_tag="KPD58_000814"
     CDS             complement(14061..17396)
                     /locus_tag="KPD58_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBG0264598.1"
                     /translation="MGNFDFLQKKKNFNSFNTACLEAEKSILVSPSTCATITRRALEL
                     AVKWLYANDSDLVLPYQDNLSTLIHNNSFIELIDYDMLPLLKYIVKLGNLSVHTSANI
                     ERGEAILSLNNLHQFVSWIDYCYSDEYTAEDFNEDLLLHGEEKRTRPDELKNLYERLS
                     SKDKKLEEVIKENEELRKSLTEKRKVNTANYDFKIDEVSEFETRKRYINIELKLAGWE
                     FGKDIWEEIEVQGMPNESGVGYVDYVLYGENGKPLAVVEAKRTSKDPKIGQQQAKLYA
                     DCLEKRYSQRPVIFLTNGFDMYIWDDYSDRKVYGFYKKSELQLMIDRRKSKKSLTSVT
                     INDEISNRYYQKEAIRAVCEALENKQRKTLLVCATGTGKTRIAISIVDVLSRHNWIKN
                     ILFLADRKALVKQAKKSFTKLLPNLALCNLLDSKDSPEDARMIFSTYPTMMNAIDDTK
                     SKDGKRLFTPGHFDLIIIDESHRSIYKKYKSIFDYFDSYLMGLTATPKDEIDKNTYSV
                     FDMENGVPTYAYEYNKAVEDGYLVDYTSIEFKTKIMEDGIKYDELSDEEKEEYENTFN
                     DDESIGDEIGNNAVNEWLFNSDTIDLVLNKLMTEGLKIEGQEKIGKTIIFAKNTKHAR
                     AIVERFNKLYPKYGGNFVKAVDYSINYVDSIIDDFSDKNKLPQIAVSVDMLDTGIDIP
                     EILNLVFFKKVRSKTKFWQMIGRGTRLCPDLLGVDMDKERFLIFDFCNNFEFFKFNPK
                     GFEGNKAETLTEKLFNIKVSMVKELQDIKYIEGEYAELRKELLEELITSVKALNEDSY
                     IVRMNLSYVHKYKNENVWSNIGAVAQNEIREYISPLITSYSDDELAKRFDVVMYNIQL
                     AYLQNNNASKGIRHVMATAEKLSKLGTIPQIQQQKYTIEKAMTEDFWEDSDIFEVEEV
                     RISLRELIKYLEKSSQKIYYTSFEDMIVAEDRNDSVYNANNLKNYKKKVEYYLNSHKD
                     ELAIFKLRNNKKITKQDVETLEEILLKQLGNCDDYKKEFGDTPVSQLVRRLVGLDREA
                     ANEAFSEFLNNKSFNTKQIHFVKLIVDYVVKNGFIEDNKVLMEDPFRTVGSIIDLFEN
                     HIEERNKLIKTINKIKENASEIG"
     gene            complement(17445..17873)
                     /locus_tag="KPD58_000815"
     CDS             complement(17445..17873)
                     /locus_tag="KPD58_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264599.1"
                     /translation="MVIRNKLSWNSIEEVNRDFGSCLYDLQNFKILYDKEEMPELWAR
                     YIKEGFKSYMVSFLELTKAMLYYKSIDLNIKSKNFYDYLLACEYHNLLPKNSSIVIET
                     LRKLRNDDSHGYDIPQFEDMYELFTENEEVFVSIRNSCKK"
     gene            complement(17863..18243)
                     /locus_tag="KPD58_000816"
     CDS             complement(17863..18243)
                     /locus_tag="KPD58_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBG0264600.1"
                     /translation="MRNIPNEIKNSIINVFPKENTLAIIIFGSYGTDKHTSESDIDIA
                     WIPIKKVPITELATKTEALRNILDIDVDLKIVTDNYTIALKKAILEGEVIYESKDFKD
                     YINKFYFENSDVIDILDWRDMYGN"
     gene            complement(18276..18584)
                     /locus_tag="KPD58_000817"
     CDS             complement(18276..18584)
                     /locus_tag="KPD58_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264601.1"
                     /translation="MNQIELDLYHNMVNIYKEADLQCNYKPTRFLQLINNKGALLAAK
                     ELINKEGATEGFTRLWECKRLDLSLEVLVLKEKYKELFTDEEREICINRLKDYGYKFN
                     "
     gene            complement(18617..19801)
                     /locus_tag="KPD58_000818"
     CDS             complement(18617..19801)
                     /locus_tag="KPD58_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBG0264602.1"
                     /translation="MVDEAKILDVVSISGENVKKFDGERSYLSTGNLDFNKISNLEIV
                     TYENKPSRANQTVNIGEVIFAKMKDTKKTLVINKTNKNIIVSTGFYVLKPSKEILPQY
                     LYHYLNSSYFLNQKNRLSKGATQSALNNEGLANIKIRMYNLKVQEKVVRVLDKAQELI
                     DKRKEQIEVLDELVKSRFIEMFGTPSKNEKNWEISEIGKYLDVLTDYHSNGSYETLRD
                     NVTLLDTKGYALMVRTTDLENNNFEKGVKYIDEHAYNYLEKSKVFGGEVIINKIGSAG
                     KVYLMPFLNKPVSLAMNQFMLRFNEDKVNHIFLYNLLLTSYMESKIKEKVRGAVTKTI
                     TKDAVRKINIIVPPIRLQNQFANFVKQVNSLKFEMETSLKELEDNFNSLMQKAFKGEL
                     FN"
     gene            complement(19805..21268)
                     /locus_tag="KPD58_000819"
     CDS             complement(19805..21268)
                     /locus_tag="KPD58_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HBG0264603.1"
                     /translation="MITGELRSKVDKIWETFWTGGLTNPLEVIEQFTYLLFIKGLDEV
                     ETKNEAEATLLGVSFERIFNDEHQHLRWSKFSNEGNSEKMYEIVQNEVFPFIKKLHGN
                     KDSAYAKYMGDAIFKIPTPLMLSKIVDGISNLELSKDRDTKGDLYEYLLSKVATAGTN
                     GQFRTPRHIIDMIVRLIKPTPEDIIVDPAAGSAGFLVSSQQYLRDNHSSLFLVQGLKE
                     HFNNGMFYGFDMDRTMLRIGAMNMMLHGVDNPNIEYKDSLSEVNTDKDKFTLVLANPP
                     FKGSLDYEAVSADLLKVTKTKKTELLFLALFLRVLKIGGRCACIVPDGVLFGSTGGHK
                     SIRKEIVDNHKLEAIISMPSGVFKPYAGVSTAIMIFTKTGTGGTDKVWFYDMKADGYS
                     LDDKRNEIKDSDIEDIIKRFDNLDGEVDRKRTEQSFFVDVEEIRENGYDLSINKYKEV
                     VYEEVVYDAPSVIMGRVKALEKEIVDGLEELERMLEV"
     gene            complement(21778..22647)
                     /gene="gatY"
                     /locus_tag="KPD58_000820"
     CDS             complement(21778..22647)
                     /gene="gatY"
                     /locus_tag="KPD58_000820"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBG0264604.1"
                     /translation="MALVTTKQILLDAQEGHYAVGAFNVENMEMVMAVIEAAEELKSP
                     VILQTTPSTIKYAGLDYYLANVKVASEKASVPVAMHLDHGSSFGLAMQALRVGYTSIM
                     IDGSHESYEDNISISKSVVDACMPSDIPVEAELGKVGGKEDDLDGGDGGAYTDPLEAK
                     EFVERTGVSSLAVAIGTAHGLYKGEPKLDLDRLSEIRDVVSVPLVLHGGSGIPDEVIK
                     ESIKRGICKVNYATELRIAYSNGVKNVLNSDPEVIDPKKYGKKGLESVKDFVKSRMEV
                     CGCVGKAIETVKC"
     gene            complement(22667..24583)
                     /locus_tag="KPD58_000821"
     CDS             complement(22667..24583)
                     /locus_tag="KPD58_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HBG0264605.1"
                     /translation="MGKKMILGVTGCPTGIAHTFMAEEALKNSARELGCDIKVETNGA
                     IGVENKLTAKDIEMADAIIVACDKNVDMDRFNGKPVIEVPVKEGIHKANELIQRCIDG
                     KVAIRKGNLSSSKIAEEGNLSFGQKLYKDLMNGVSHMLPLVVAGGVLTAISFLWGIYS
                     FDPNSEQYNQIAATLKSVGGYSMNLMVPVLAAFIAQSISGRAGMLAGLVGGMISFDTG
                     SGFLGGIISGFLAGYLVKLFVHLLRKLPRQLEGLKSIFIIPIVSVGLVGVAMLLLGGP
                     CSALNNAMMNFLSGLQNSSPIILGIVIGCMSAFDMGGPVNKAAYVTGTMLLGQGNYLF
                     MAGVSAACITPPLVIAIASTLFKNRFTEEDRAAGLINYILGSTHITEGAIPFAAKNPL
                     KVLPVLMIASSISSILTYIMKIEVPAPHGGFLVLGLVNKPLLWVGCIFVGSLVGAVLY
                     MMVTPKVVNEKNTIENTNATKDEHKNESAESENVSNNISLYSEETVILDVKGKNKTDV
                     IDEMIGILDKSGVLLDKKKFKEEILKREEISSTGFGMGIAIPHAKTDAVKVPRVAVGV
                     SKEGFDFESEDGSLAHIIFMIAATDNGDNLHLKTLSQLSSKLMNEEFLNELLNSKTSR
                     EIVSKLNNEEIKSV"
     gene            complement(24586..25506)
                     /locus_tag="KPD58_000822"
     CDS             complement(24586..25506)
                     /locus_tag="KPD58_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HBG0264606.1"
                     /translation="MIYTLTTNPAIDMNISTNGIKRKLVNRTSNAVYSPNGKGLNVTF
                     VLGHYGIESKILGFFGGFSGKYIVEESEKRGFDVLPTWVEDTTRINIFLNDGSDEFKF
                     VNSGSYVNEKQKLDMLDKIQSLEDISYLSISGSLPPGIDDNYYEDIFKICKNKSIKTI
                     LDISSPKLKELLQYNPYLIKPNDEEIKDIFGIIVRDEEDIKDVLKLLHLKGAQNILLT
                     LGEKGSYFYNGKSIYYASAQPVTVVSSACAGDSALAAFLSIWLENPEHIEEALKRSAA
                     TGASVAESNGIGNLENVEDYIKNIRVRKVV"
     gene            complement(25608..26360)
                     /locus_tag="KPD58_000823"
     CDS             complement(25608..26360)
                     /locus_tag="KPD58_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG0264607.1"
                     /translation="MIIELNKSNSENLTKTELEIIKFINENESILPELSIVEIAHETY
                     SSPATVSRAIRKCGLNGFNELRYRLTVKDENDDIQNMGDIINKSFIEAQRVIEQISLT
                     TLINIIKTINKSSKIYILARGLTEYVAKEFSLKLQLLDFNVVFINDPNIMKIKSKSIK
                     KDELIIIFSLNGNTIELVESAQNANLVGAKVITCCCSESAKLIKYSDLYIVGYKHEHI
                     SISEYEVSSRLPLYIISRIIIDYMVRYNNSEN"
     gene            complement(26752..28197)
                     /locus_tag="KPD58_000824"
     CDS             complement(26752..28197)
                     /locus_tag="KPD58_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBG0264608.1"
                     /translation="MIKLGKFPKGFLWGGAVAANQLEGAWNEGGKGWCLADVHMYDKD
                     RDISKPYESDMTIERIKFAMKDKNGYYPKRSGIDFYHTYKEDLKLLKALGLKCFRFSI
                     NWARIFPNGDDLEPNEEGLKFYDNLINEVIDNGMEPIVTLLHYETPLNLVLKYKGWSN
                     RKVVDFFVRYAELILNRYKNKVKYWIVINQINLIHYESFNSVAFCVDQVDNVEEARYQ
                     AIHHQFIATALTKKLGKEINSDMMIGMMMADCTSYPEDCDPENVVFALKRNRLQYFFT
                     DVSFRGEYPVYIKRFFKENNINIVMEEGDDKILKENTMDFLALSYYYSTTVSASKNTM
                     NPTDNIKNPYLKANPWGWAIDPKGLYNTLSQYYDRYQKPIMIAENGFGMHDKFEDGKV
                     HDSYRIDYLGKHIEQIGEAIADGTEILAYCMWSPIDIVSCSSQEMEKRYGFIYVDIDN
                     NGNGSKERYKKDSFYWYENVIRTNGEELREK"
     gene            complement(28219..28572)
                     /locus_tag="KPD58_000825"
     CDS             complement(28219..28572)
                     /locus_tag="KPD58_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG0264609.1"
                     /translation="MTIEEHIIDEELVEVAMQIILRAGEARTEIKHALNDLEKFDYRN
                     ADLKLAKAKGFMTEAHRAQTKIIQGEAGGEKRAHSLLFAHAQDTLMTIFSELNITMSL
                     VGIVKSIEYKVNNSK"
     gene            complement(28601..29848)
                     /locus_tag="KPD58_000826"
     CDS             complement(28601..29848)
                     /locus_tag="KPD58_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0264610.1"
                     /translation="MGRVFMNKFIDFLSEKFTPVVNNMTKNIWVQSVQSTIMKVLPMV
                     FVGSLVTIVSVLKNYISFLPDLSPINQYTFGLLGLFIAFLLPMEIMKNKKFESMSVVA
                     GLAGAGLMLMMIRPEITNEGAIFNFNRFGGEGMLVSLVAGLFSGLIMSLFGSFSFFGE
                     DSALPDFVSKWFDNMLPITVIMFVGWGLIYGLNFDMFEIIVRVFKPVSDIAQTLPGFV
                     LLNFLVVFIYSLGISGWVLSPITYSIQLGAIAANAAAVASGGVATNIYVYEVIQTGWI
                     TLGGLGATLPLVIMMCLSRVGRIKAIGRACIVPSIMNINEPVIFGAPVAWNPFLMLPM
                     WIGTVVISITTYLSMSMGFVTIPSKVFNLWYVPFGISTFMVNQDVRGLIVLAINIVLL
                     FMIWYPFFKAYEKEEIKLESQEV"
     gene            complement(29856..30173)
                     /locus_tag="KPD58_000827"
     CDS             complement(29856..30173)
                     /locus_tag="KPD58_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0264611.1"
                     /translation="MKNIRVLLACGSGASTGFMATSMRKSAKKKGIEADIKARSESEI
                     DKYLSEIDCLLLGPHLDYMKEEIQQKANEYNVPVACISKTAYGRLDGAKALEEALELI
                     SNK"
     gene            30415..>30943
                     /locus_tag="KPD58_000828"
     CDS             30415..>30943
                     /locus_tag="KPD58_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBG0264612.1"
                     /translation="MKTSKKELFLNFIKTQHDWIDSSTLANYLNVSTRSIRKYVNEIN
                     SNGEFILSSKKGYKVNLNNNCQTKVDSSENISPDNRLNLILKELIVNSNGINIFDLSE
                     ELFVSPATIEGDIVKANKFIGSYNLKIKQSKFLLKLIGNESAKRKLMSSIIFKETGSD
                     FLSLFDVQKIYQEYNL"
BASE COUNT         9852 a         5668 c         3254 g        12169 t
ORIGIN      
        1 ttaaatttag atactttact gatttttatt tattagttga gaaagatatt gataattaaa
       61 aatattgata actatagaaa tgattatttt ttatagaaat tttaaggata taaactaatt
      121 atacttaagt ctatatcctt aatttatcat gattatgaga atttttacaa atacttatct
      181 ttgtccgttt tagaataaaa acaagaacat atcctgttat tttattcaaa catccaagtt
      241 taattactac acaattactt attagaaatt gtatctactt catatatata tctgttaagt
      301 atagcttcta gcatttcttg tcttccagac ttattcttta taactggcat ttgcagtgca
      361 taagattcta actctttaaa tccaacctta ccttctacta tatccttacc tataccttca
      421 ctaaaacttg catatctttc tttagtaaag ttctcaaata cctcatcttc taataattta
      481 tgagctatta ataatccctt tgcaaatgta tccattcctg caatataagc taggaataag
      541 tcttctacct caaaagatgc tcttcttact ttagagtcaa agttaaatcc tcctggtgct
      601 attccacctt gctttaacac ttcatacata gcaagtgttg catcatatat atttgttgga
      661 aattggtctg tatcccaacc taataatagg tctccttgat ttgcatctat actacctaaa
      721 acattgttta ttcttgctat atttaattca tgttggaaag tatgtcctgc aagtgttgca
      781 tggtttgctt ctatattcat tttgaagtat ttatccagat tatactttct taaaaatcct
      841 aaaacagtag cagtatcaaa atcgtattga tgtttagtag gctcttttgg cttaggctct
      901 attaagaatt gtccactgaa tcctatttcc ttagcataat ctacagccat ttgtaagaat
      961 cttgcaaagt tatccatttc aagttctgta ttagtgttaa gtaaagtttc ataaccttct
     1021 cttccacccc agaatacaaa attttctcct cctaagtact tagttatttc catagctttt
     1081 ttaacctgag ctgctgcata tgcatataca tctgcattac atgttgtaga tgcaccatga
     1141 acaaatcttg gatttccaaa catattggca gttccccata ataactttat acctgtcttt
     1201 tccatattaa cttttatata ctcaactatt tcatctaata tcttatttgt ctcttctaaa
     1261 tctcttcctt ctggtgctat atctctgtca tgaaaacaga aatactcaat tcccatctta
     1321 gacataaatt caaatcctgc atcaacttta atctttgcta tctcaattgg gtctgttata
     1381 ttattccaag gtctctctac tgtccctact ccaaatgggt cattaccctc tccacataaa
     1441 gtatgccaat agctcattgc aaatcttaag tgttctttca tagtcttatt acctaccact
     1501 tgttcaggat tgtagtatct aaatgctaat tcattgtcag attttactcc ttcaaatttt
     1561 atctgtccta ttcctttaaa tatttcactc atttcatgtc ctcctaatat tttacacata
     1621 taatatatgt agtatgtttt cttaatttaa aactattttc tatctttatt tttcttttta
     1681 agctaattta attatttatt tttgcattaa ctacttcatc taataactta aagtcatctt
     1741 tcaaagattt gtatattctt ttatacaatc tatgatatat attgtatgta tctacatttt
     1801 cacttattgg ttctaattta gttgaagttt ttattatagc atcacaagct ttttgtatat
     1861 tttcatatat tccagctcct actaatgcaa gaattgcaac tccaagtgct ggtccttcac
     1921 ttgaatttat agtatataca tcttgattaa acatatctgc ttgcatctgt ctccatactt
     1981 tactttttcc acctccacct gaggctctta cttcattaac gtctatatta agattctcta
     2041 ttatatccat acagtcttta agactatatg aaacaccttc cattattgac cttgcgattt
     2101 cttttttaga atggattgga gataatccaa agaacacacc tcttgcataa gggtcaagat
     2161 gaggagttct ttctcccatc atatatggta agtaaagcaa tccattacat cctgttggta
     2221 ctttacttac ttgattattt ataatctcat atacatcttc acctaaagaa tttgaaactt
     2281 ccacttcact ttgacagaaa ttatctttaa accattttaa tgacaagcca gctccttgag
     2341 taactcccat aacatgccat gtatttggta ttgcatgaca gaaagtatga actctacctt
     2401 ctttatcaat ttttggctct tttgtataag caaatactac acctgatgta cctatagtag
     2461 atgaaactac tccatcttta actattccat ttcctatagc tcctgctgct tggtctccag
     2521 ctcctcctac cactattgtt ccttctttaa gccctgtcaa acttgctaca tctctagtca
     2581 cataacctgt aatttcttgt gattcataaa ctttacctaa aagattttta tctatattta
     2641 acttttctag tacttcatcg ctccaatctc tttttcttat atcgagcaac tgcataccac
     2701 ttgcatctga tacctctgta gcatattcac ctgtcaattt gtatcttata taatcttttg
     2761 gaagaagtat tttattagta tttttatata tatctggttc attgtttcta acccataata
     2821 tctttgatgc tgtaaatcct gtaagtgctg gattagcagt tatttcaatc agtctttctt
     2881 cacctactaa atcagttatc tcctcacact cttttactgt tcgttggtca caccatatta
     2941 tagagtttct caatgtttta ccttcattgt ccagaataac tagaccatgc atctgtcctg
     3001 ataatccaat tccttttata cattcactgc ttatatttga actttgaact acttctctta
     3061 tagattcgac tacagctttc caccaatcat ttgcatcctg ctctgcccaa cctgatttct
     3121 cttgtataag aggatattca tatgtacaac tttttatagt atttcctagt ttatcaaata
     3181 aaactgtttt cgtacctgat gtaccaatat ccacacctaa tacatactcc aacttcatcc
     3241 ctccttgaat gccacttcat aactatcgtt ttcctatcct aattttttta tattttaggt
     3301 atattttact tgctttattt ttgttagtat attaaactaa cttatgaact aataataaaa
     3361 tatttaatta tctttgtcaa tgcaaaataa ccttttattt ttgtgtaaaa agtattacaa
     3421 tattatttta aggaggaata tcaaattggt tactgataaa tacactattc gagaaatgaa
     3481 tgaaagattg gtacttgaac aaattataaa aaatggtcct atttctaggg caagtatagc
     3541 ttctaccata ggattgaata aagcaactat atccgctatt accaaaaaac ttattgatga
     3601 atctctagtt catgaaatcg gaattggaaa tagtactcat agtggcggaa gaaaacccat
     3661 attattagta tttaataaat gtgcagggat ttctttaagt atggacattg gatacgacta
     3721 tatattttct tctctatctt acttagatgg aactattata aattctaaga aattaacaga
     3781 tatacaagta agtaaagaca atgttataca attgatagat gaaattataa atagttataa
     3841 catatccaaa atagacactc cttataaggt aattggtctt actttggcta tacatgggat
     3901 tacttgtgaa aataaagtat tgtttactcc atattacaat cttaatgaga ttgacttata
     3961 tagcatcctt agcaaaaagt atgactttcc tattcacata gaaaatgaag caaacttaac
     4021 agctttagct gagaatactt tttcaactgt acataactca ctacttagtc tcagtattca
     4081 cagtggattt ggttcaggca ttataataaa taataaatta tatagtggca gaaatggtat
     4141 gagtggagaa attggtcaca ctatcatcat gcctaacggt aaactatgtc cttgtggaaa
     4201 tcgtggctgt ttagaacagt attgttcaga aaaaaaggta tttgaacagc tttcttcttt
     4261 agaaaatatt cctaaaatag attctgatat agtaaagcaa ctatattatg aagataacca
     4321 gaatgccaaa aaagttattc atgagttttg tagttactta acaatagcta taaataatgc
     4381 tattacaact tatgctcctg aaataatata tttaaatagc caaataatta gtgatatccc
     4441 tgagatatta cagattacaa aagatatgtt agttagctct tttaacaaag gaataaatat
     4501 agaaatttca tcattagggt ctgaggcatc tctatatggt ggctctgccg taaatatcaa
     4561 gagctttttg aatatacaaa atttaacttt aattaatgaa atataagggt tatgactata
     4621 tacagccata accctttaaa ttatacattc taaaattaga tttattatat agtttatgca
     4681 agattagcta tattttttaa aaattgtata tcaattaaat attaacataa gtatttcgtt
     4741 ttaaatatta atataatctt cctactcatc atcagataat aaacttgcat tttgttcaac
     4801 catagaaata ttatctttac ctattttctc taattttgct gccaattcct ttgtatctaa
     4861 aaaagctgat tcaataacca tacctaaatt tagccctgaa ataattttaa ttcgactgtc
     4921 attattggct actaataaag cagcattgca tggagttcca cctagtaaat ctgccattat
     4981 aattatttct gaactttcct tgtacacttc taaagctttt tttaattttt ctattgttcc
     5041 ttctattcca tcatcttgtt tcatgtttac agtaggataa tctatagaat cccctattat
     5101 catttttgca gaatttaaga cttcttcagc catattgcca tgactcatca aaataatact
     5161 attcaattta atacacctct ttaaatctat aagatgtcta caagataagt tttcttatca
     5221 tcaggcgtca ttatagttgt aatttcaatt ccttcatcta aaagtctttt aaatgcttct
     5281 ttctcttctt cagtaacact gatattaggt cttagaactg ttgtatcttt tctacttgac
     5341 atatttccta catttatatt atgtatttta aatcctgcct ctacaaatgg aataaggtcc
     5401 acaggagact taacaattaa taatacattt tgtccttcat actttcctgc agtaatattt
     5461 tttatagcag tctcttctgt aattatagat gacgctatac ctgcaggtgt agccattctt
     5521 aaaacttgtt tttgaacaga attgttagct actgcatcat ttactaccat taatcttgta
     5581 acacccaatt tattactcca catagttgca acttgaccat ggattaaacg gtcatctata
     5641 cgaatatttt taataccttt catttccata tctaatatca tcctttcctt attttaaaat
     5701 acctaatgca tataaaatta ttgaaacaac cattactcct aaaattaagt atataggttt
     5761 tatattcttt ttaagagccc aataaacaat cacaactaaa attgcaggaa ctaacccagg
     5821 cataatttgg tctaatattt cttgaccttt cattgtaaaa tcaccttgtt gaaagttcaa
     5881 agcaacatta gcttttacaa ctgaaggtat taatgcacct acaaccatta aacctaaaat
     5941 agatgtactc tcagttaaaa tctttaaact tttttcaatt gaagatatta gcttagtacc
     6001 tgatgtataa ccagcattaa atagtggtat acttaataat ttcaatgcta cactcactac
     6061 aatccacagt aagattccaa atggacttcc atctatcgcc atattagctg ctatagctcc
     6121 aaaaattgtc cacggaataa cgacaaataa actatctcct aaacctgcta ctggtcccat
     6181 caagcctgtt ttaattgaag caacagcctc ttcagtctct tcacttgata actcctccat
     6241 tgctacatta atacccaaaa tataaggggc aacccaagga ttagtgttaa aaaattgtaa
     6301 atgatttaat acagcacttt gtaacttttc tggattatct ttgtatacta cttttaaaaa
     6361 aggtagtact gcataacagt atgccaaggc ctgcatcttc tcataatttg aagaaacaga
     6421 actagtaaaa aaccagcgcc ataagacact ttttttcacc ttttcatttg atttatttaa
     6481 tgatacctta tcactcatct tcgtccatac ctcctactac agtatattgt tgttcttgat
     6541 tttccaaatt ctttttgtaa attataagtg caattgctcc accaattaaa gctacaccta
     6601 ataatggtac tttcatatat actgctaaaa caaaaccaat tactaagtaa ctaaaatact
     6661 ccttagcagg aagatatcta agtaataatc ctatccctac tgctggaagt agtcctccag
     6721 ctactgttaa accacctgtt aaccattcag gtgtatattt caaaatggta ttaattaaag
     6781 aaggtccaaa tattacagat agagcaactg gaatacctgt tgtagctgct gttaatagta
     6841 ctcctaacat ttgcatatag gttatatttt tataatttcc ttttttagct ccatcttctg
     6901 ctttatgaac tagccatata ccaatagtat ttctaagaac atctaattgt accatcaata
     6961 aagcaactgg aatacctata gagattccta cagatgcttc ttgcttagtc gtaatagtta
     7021 taaatgtggc tactatactt gctgtttgat aatcaggtat tgatgctcca ccaaagttac
     7081 taatacctaa tgataatagc tgtaatgtac ctccaatata taaccctgta tttatatctc
     7141 ctaagataag accagtaata aaacctgctg ttactggttg atatgttcca aacattgttg
     7201 aatttttttc atagtttata aaaaatccat atagtacgat taataataat tgccataatt
     7261 gtggctccat tatgatttca cctctcccaa aatatttttt agttctttaa aattataact
     7321 ggaatcttgc tttaggaaaa taggaatttc ttccagtggg cattcaactt cataagtttt
     7381 cccaccttca taactaactc cactaaatat attttcccat gtatgtcctt ttggtaaata
     7441 tacttttcta cttctatctt tataattcat tattggagct actaaaattt catctccaag
     7501 catatatgta ttatccacct cccaagtttt atcatcttca ggaaattcat aaaataaagt
     7561 tctcataact ggtgttccat cttcatgcgc ttctttcatc agttttttta aataagtttt
     7621 taagctctct ctaattttta tgaattttgt aagaattact tcaacttctt caccataaga
     7681 ccatatttca ttaggagctc cagttaccat tgagcctcca cctttatttg ataatggttt
     7741 actatgaggc aatctatctc catgcattct caaaattggt gaaaaagtag catattggaa
     7801 ccatcttacc ataagttctc taaattctgg gtctttagga tttcctccat ggaaacctcc
     7861 aatatcagta gtccaccatg gtatacctgc cagtcccata tttaaccctg tatttacttg
     7921 attattaaaa gcctcaaaac tagaatcaat gtcaccagac catactaaag ctccatactt
     7981 ttgagctcct gcccaagctc ctcttacaag agttacaaca gactctatgt cattatgtaa
     8041 accatcataa atcattttta aataatcaat aggataaata ttaccacaac ttaaaacatt
     8101 tcctttttta tatctatagt tatcaaaatc atacacagaa tagccaggtt cagctacatc
     8161 taaccaataa tagtcaatcc caagttgttt ataattttta tctattctat cccaaacata
     8221 ttctcttgca ttttcatttg tcatatctac aaagactgta tttccttgaa tttgcattgt
     8281 cattctaaca ccacgattaa cattgactaa atagccattt tctaaataat cattgtagtt
     8341 ttctgcatca ctttgaactg taggccatac agatactatt ggttctactg aatactcttc
     8401 tttcaatttt ttaactaatt ctttaggatt tttccaataa tctaagtcaa atttatattc
     8461 accttgctta ggccaatgaa aatagtcaat tgcaattgat gaaagcttaa ttcctctttt
     8521 agaatattcc ttcacaacat ctaaaacttc ttctgaagtt ctatatctta gtttactttg
     8581 ccatagtcct aaaaggtttt caggcatcat aggaacagtt ccagtaactt gagaataatt
     8641 tttatttaac tcttttgggc tttctccaca agtaatccaa taatctataa aatttgtaga
     8701 aaacatcttc cattctgtca tatttttagc aaaagatact tttccaattc ccggattatt
     8761 ccataaaagt ccataaccta gactagaaat ataaaaagga actgatattt gagaatttct
     8821 ttgggctaac tccaaaacag tattctttaa atctaaaaat ttatgttgat attgtcccat
     8881 tccaaaaatc ttttcagaag gctcagattc aaatctagta gttacttcaa attctccact
     8941 atagcttggt cgatattctc ttgattttaa ctttagagta gagttaaagt ctttggtaat
     9001 ctcaattgtc cctacatctt ctccaccatc atcatgtttt acagctctta atcgaatata
     9061 ttctttcaat aatatttcat ttttatcatt ataaaaagta attctatctc tatggtctaa
     9121 gacagctttt atctttccat ttttaatgat tgcacttcca tcttcatttt tattaattgt
     9181 aatatcatct ttatttaact taggtttttc gttaagagca tagttttcat caacaaaatt
     9241 ttggtctaca aacgacctaa tacgtaaaga gttttttccc catgcttcta ttcgcaatag
     9301 ttcattgtca aatctttttt caatatagtc ttcagatatt tttatcacgg ctacctccta
     9361 aatcaatggt ataatattct atctttgtgt agcttaagtt aaataatgtc gtttagtatc
     9421 gttatttata aattaggttt tatatataat tcatttgtac attaaactaa cttaccaata
     9481 atatacttta tttgactttg tttgtcaatc gctttccgga ttttagtaaa attttctaat
     9541 atgttgcttg aaagtcgttc ttaatatctt ttatttatac ttttaatatc tgtgatgata
     9601 cctatataca tctagttttt ttaaaacaca tacagaaata aagtttgcct attatgtaga
     9661 taatatcgga cttgaaaata tgtatagtga ccttataaat acagataata atatattaga
     9721 tattgttcaa aacaatggat tttataatta taaataattt atgaaattat ttaataaaat
     9781 atataattgt ataccaagtc aaaaacgaca acaaataaat aaaactaaaa aagatgttgt
     9841 ctcaaaatat agataaattt atttttcatt gctaaaatga ttaaaatata tttgatttaa
     9901 tatacttgat ttaatataaa aaaagagtgt ctcataaact aaaaagttca cttttagaca
     9961 ctcctttata tatattatct acttcttaat tcatttatta cctttctaca ttttctttca
    10021 gctagtggat acatctttat aagtactaat tactccactt aaaaatggtt cctgtactgt
    10081 ccatcagtca tatttttctg ccaaatataa tggtgtttca taatgagaca ataaatattt
    10141 atatctctac ttatcttctc taagctcgtc aaatccaact ataggtatct ctaaattata
    10201 ttctttaagt agcttttcta gctcttctat aaaaatacct ttcccatcca ccatttgtat
    10261 atcatttatt ttatccatca attcattgca accacttaac tcaccaaacc aacgtttaga
    10321 actacatgtc ttattataac cacaacttgg actgccatct attgcaatga tactttttat
    10381 atcataatca ttttttaaat attcatcaaa ctgccttaca tgaggtatta acatttgcct
    10441 acattgttct ctaaaaaaca aattgtcaaa ctgttctttt acatgtcccc acctatttat
    10501 tccatacata atcatttctg gacatggtag ttgaattatt ccataccctt tatttattaa
    10561 atctattgct atattttgtg ctcccttaaa ttctgacaat ccctctactt tagaattaca
    10621 atttaatata caatgacata ctactacaat ctctctattt ctcttcataa taatctcctt
    10681 agttattctt aattttaatg atactacata ttttgataat tatattattt atttgttcta
    10741 tatttccaat attctatata gaaatataca attagtaatc ttttataatc tatactatga
    10801 taaatgtata tattttttta ggaggtatta taattggata ctaaaaaagt cacattttct
    10861 tcaatgtgca tagttattaa tattgtctta ggtatggtta tatctatgat aaatatacca
    10921 ttattatttt tagatacagt tggtactgta cttggggctg tcgtattagg tcctttttgg
    10981 ggtgctctta ttggtggatg tacaaactta gttttaggtg ttatatcagg tcctacaaat
    11041 ataccttttg cccttgtaaa tattgttctt ggtcttattg ttggatatat ttctaagaaa
    11101 aaaggttttg gttataaaga agctattatt acaggagtaa ttttatctat tgtttgtcct
    11161 ttaattggta ctcctatttc agttttattg tttggtggtt taagtggaag tggtgctgat
    11221 ttattggttg gttttttagt tcagtctggt cagaagatat ttactgctgc gttctttcca
    11281 aggattctta gtaatatagt agacaaacct ttgtcttgta ttatggtggt atattttata
    11341 agtaaaatgc ctaaaggttt tataaaacaa tatagtaaaa atacttctag ttcatattaa
    11401 taaatcattt attatgtaag gtttattact ctttaaaatg ctatatactc atttttatat
    11461 aacattttta ttaataattt aattagatag tatttgtatt actttaatta aacgtaacct
    11521 cttgatataa atttgagcta taaatctata tcaagaggtg ctattttatt tttaatataa
    11581 ttaatattct attacgcaaa aaatgcatat atttcattat tttatggaaa atattacttc
    11641 atttaactct atatcaaact cattaagtaa aaatctaatt atatttaatt tagcctctgt
    11701 atttaaattt gtttctaaat atatattttc tgatatttta ataccttttc ttaatttata
    11761 tgcatctcta gacttaatat tatctataaa atctctttta taagttttgt ctacaatact
    11821 gttaaacctt acagaatcca aatcatataa agtaagagca acttgttcat aaaattctgt
    11881 ccaattttta gccttatatg actctcctaa tatactaaat gatactatac tttcatttgt
    11941 aaaattaaca tcatcatcct ctaacgtaaa tatattttca ctgttacttg gaggttcata
    12001 tgtaacactt ggatatttcc aaatttctac agctctatct agtaatatct tagttctatt
    12061 ttttatttct tcttcattcc acttatctat agatgaaata tatttattta gataaagtct
    12121 actatcttta aatccatctt ccatttcttt cttttcttca aatgttttat tacttagttt
    12181 tgaattatat cctgtcaaag ttaaatttcc aattgtatgt aaatatttat catgtatctc
    12241 cttatagtta tctcctagtg attccttcca tttatttgtt aatgtctgtg gcattatatg
    12301 ctctatattt agcatattat tattgattaa attttctaaa tcaacaattt ctttattatt
    12361 ataattctct aacctctcaa gtatatataa cttattcttt gacttagtat tatatatatc
    12421 tttagtttca aaagcatttg aaaattcact gtcagtagga aatctttgag atgatttctt
    12481 atttataaaa atatatctta atatattaga atagttttct ttataatcac taaatttttt
    12541 aatttccctt cctaagttca taaatacttt attcagtgca ttaggaggta catcacatac
    12601 tattcttctt aatataaaac attctactaa attaagggat tctacaagta cttttttatt
    12661 tattatacct tcattataat catcaaataa ttctaatata aacggatatg atacaaatat
    12721 ttcaagtttg tttatccttt tcaaaacttt gtttacatca ttatcatcag tacatgaata
    12781 agatatctct ctataatagt tagaaaattt aagtaaatct tttaataaaa tttctatatc
    12841 tataccatta tctgatacat accttttaaa actaaaatat actttatttt tattcggtat
    12901 acttctctcc tttatagtca aatagtctct tataaaacta cttacgttgc tacttgtatt
    12961 tttttcaatt ttataccaat aattattata aaattcctct tgtaacttag agcttctacc
    13021 cattaacaca aagtttctta ctctatcagc ttcacttaaa tctaagccag ttgaatttag
    13081 actttcaaag attaattgag ggtcatcttc accatttttt aactctattt ctactatagt
    13141 taatttctgt atagcatcaa acaaatcatc tattgatata ttatcttctt ttattctatt
    13201 ataaaaatat aaataatttg aaactatatt agaatttcta tctgtattca tttcttcttc
    13261 aaatagttca tagtacgctt ttctatcttc tgctattggt tttaacttta tttttccatc
    13321 tttatctctt gagtgcttgt ttataagata acactcctta atctgttctt ctgttgtaat
    13381 aattcctttt gattccccaa actcttttat tatttgacac atggcaatca ataatattga
    13441 cacagtagtc actctttgtt gtccatctat tattaaatat tcttttccca tactatatat
    13501 agatacaact gaccctaaga aatgagttct aaatccagaa tctattatat cctctaaatc
    13561 atcaaacaat cttttacatt gttctttctt ccagttataa tttctttgat atacaggtac
    13621 tacaaaactt ttgtttgacc cctctaaata actaagaaat tcaatttctc ctgctttcat
    13681 attaactccc cctataagtt aaataaattc cattcataac tccatgtatc tatatattat
    13741 atcaaaattt tgactcataa atgtatatga aagccttatt atataccttt ttatacctgt
    13801 ttttatttta ttaaagtaaa tacatgatta ttctataaaa tatacaaata cttaatttta
    13861 aaaattatta tttaatcttc ttcatgagta attcatagtt atttatcaat taaaaagaga
    13921 tattgcataa ttattcaata cctctctttg tatatttaca ttatccatat ctagttatct
    13981 atttcaaccc aatattagac tacctactaa taaatttatt ctctacatca ttaacaataa
    14041 ttttgcacta aataacatca ttagcctatc tctgatgcat tttctttaat tttatttata
    14101 gttttaatca atttatttct ctcttcaata tgattttcaa ataaatcaat tatacttcca
    14161 acagttctaa atgggtcttc catcaacact ttgttatcct ctataaatcc attcttgact
    14221 acataatcga ctataagttt aacaaaatgt atttgcttag tattaaagct cttattattt
    14281 aaaaactctg aaaaagcttc atttgcagct tctctatcca atcccactag tcttctcact
    14341 aattggctta ctggagtatc tccaaactcc ttcttataat catcacaatt acctaactgt
    14401 tttaagagta tttcctccaa cgtttcaaca tcttgctttg ttatcttttt attatttctt
    14461 aatttaaata ttgctaattc atctttatgg ctatttaaat aatactctac cttcttttta
    14521 taattcttaa gattgtttgc attatataca gaatcatttc tatcttctgc tacaatcata
    14581 tcttcaaaac ttgtataata tatcttttga ctagactttt caagatactt aataagctct
    14641 cttaatgata tacgtacttc ttctacctca aatatatctg aatcttccca aaaatcctct
    14701 gtcattgctt tttcaatagt atatttttgt tgctgaatct gtggtatagt cccaagtttt
    14761 gaaagttttt ctgctgtagc cattacatgc cttatacctt tacttgcatt attattttgt
    14821 aagtatgcaa gttgtatatt atacataact acatcaaatc ttttagccaa ttcatcatca
    14881 ctatatgaag ttatgagtgg tgatatatat tctcttatct cattttgtgc tactgctcca
    14941 atattgctcc aaacattttc atttttatac ttatgtacat aacttaaatt catcctaact
    15001 atataactat cctcatttaa agcttttaca gaagttataa gttcttctaa caattctttt
    15061 ctaagttctg catactcacc ttctatatat ttaatatctt gaagttcttt aaccatactt
    15121 acctttatat taaataattt ttcagtaagt gtttctgctt tatttccctc aaatccttta
    15181 ggattaaatt taaaaaattc aaaattatta caaaaatcaa atattaagaa tctctcctta
    15241 tccatatcaa cacctaataa atctggacat aatctagttc ctctacctat catttgccaa
    15301 aacttagtct tacttctcac tttcttgaaa aaaacaagat ttagtatttc aggtatgtca
    15361 attcctgtat caagcatgtc tactgaaact gctatttgag gtaatttatt cttatcagaa
    15421 aaatcatcta ttatagaatc tacataattt atactatagt caactgcttt tacaaagttc
    15481 ccaccatact ttggatacag cttattaaat ctttcaacta ttgccctagc atgctttgta
    15541 ttctttgcaa aaattatagt cttaccaatc ttttcttgtc cttctatttt caacccctct
    15601 gtcattagct tatttaaaac taaatctatt gtgtcagaat taaatagcca ctcatttact
    15661 gcattattcc ctatttcgtc tccaatactc tcatcatcat taaatgtatt ttcatattct
    15721 tccttctctt catcagaaag ctcatcatac ttaattccat cttccataat cttagtctta
    15781 aactcaatac tagtataatc tactaaatat ccatcctcga ctgccttatt atactcataa
    15841 gcatatgtag gtactccatt ttccatgtca aaaactgaat aagtattttt atctatctca
    15901 tcctttggtg tagcagtaag acccattaag taagaatcaa aataatcaaa gattgactta
    15961 tactttttat aaatacttct atgcgattca tctattataa ttaaatcaaa atgaccaggt
    16021 gtaaacaatc tctttccatc cttagacttc gtatcatcaa ttgcattcat cattgttgga
    16081 tatgtagaaa atatcatcct agcatcttct gggctatctt tgctatccaa taaattacat
    16141 aacgccaagt taggtaatag cttagtaaaa ctttttttag cttgctttac caatgctttt
    16201 ctatctgcta gaaataatat atttttaatc caattatgtc ttgaaagtac atcaactata
    16261 gatatagcaa ttcttgtttt tcctgtacca gtagcacata ccaaaagagt tttgcgttgc
    16321 ttattttcaa gagcctcaca tacagcccta atagcttctt tttgataata tctattggat
    16381 atttcatcat ttattgttac agaagttaaa gacttcttac tctttcttct atctatcata
    16441 agttgtaatt ctgatttttt ataaaatcca taaactttcc tatctgaata gtcatcccaa
    16501 atatacatat caaaaccatt agtcaaaaat attaccggtc tttgactata tctcttttcc
    16561 aaacaatctg catataattt agcttgttgt tgaccaattt tcgggtcttt actcgttctt
    16621 ttcgcctcaa ctactgctaa tggcttacca ttttcaccat aaagaacata atctacatat
    16681 cctacaccac tttcattagg cataccttgt acttctattt cttcccatat atcttttcca
    16741 aattcccaac ctgcaagctt taattctata tttatatatc tttttcttgt ctcaaattcg
    16801 cttacttcgt ctatcttgaa atcatagttt gcagtattaa cttttctctt ttcagttagt
    16861 gactttctta attcctcatt ttcttttatt acttcttcaa gctttttatc ttttgaactt
    16921 aatctctcat aaagattttt aagttcgtct ggtctcgtcc ttttctcttc cccatgaagt
    16981 aataaatctt cattaaagtc ttctgctgta tattcatctg aataacaata gtctatccaa
    17041 ctaacaaatt gatgcaaatt gttaagtgaa agtattgctt ctcctctctc tatgttcgca
    17101 ctagtatgta cagataaatt acctaattta actatatatt ttaataaagg taacatatca
    17161 tagtctataa gttctataaa actattatta tgaatcaacg tactgagatt atcttgataa
    17221 ggcaatacta aatcactatc attagcatat agccatttta ctgcaagctc taaagctctt
    17281 cttgttatag ttgcacaagt agatggacta actagtatac ttttttctgc ttctaagcaa
    17341 gcagtattaa aactattgaa atttttcttc ttttgtaaaa aatcaaaatt acccaagctt
    17401 ttatccccct tttgtccctt agttaaaata aatctctcta aaaactattt cttacagctg
    17461 tttcttatac taacaaatac ttcttcattt tctgtaaaca attcgtacat atcttcaaac
    17521 tgtggtatat catatccatg agaatcatca tttctcaatt ttctaagtgt ttctataact
    17581 atagaagaat ttttaggtaa taaattgtga tactcacaag ctaaaaggta gtcataaaag
    17641 tttttagatt ttatgtttaa atcaattgat ttataataca acattgcttt tgttaattct
    17701 agaaatgata ccatatagct tttaaagcct tcttttatgt atctagccca taactcaggc
    17761 atttcttcct tatcatataa gattttgaag ttttgcaggt catacaagca agaaccaaaa
    17821 tccctattta cttcttctat tgagttccaa gataatttgt ttctaattac catacatgtc
    17881 cctccaatct aagatatcta ttacatcact gttttcaaaa tagaatttat ttatataatc
    17941 cttaaaatct ttagattcat aaataacttc accttctaaa atagcttttt taagtgctat
    18001 tgtataatta tctgtaacaa tttttaggtc tacatcaatg tctaatatat ttcttagagc
    18061 ttctgtctta gttgcaagtt ctgtaattgg tacttttttg ataggaatcc aagctatgtc
    18121 tatatcactt tctgatgtat gtttatctgt tccataacta ccaaatatta tgattgctag
    18181 agtattttct tttggaaata catttattat tgagttttta atctcatttg gtatatttct
    18241 catttaatca cctcttgttt attaggtaat attacttaat taaatttata tccataatct
    18301 tttaatctat ttatacatat ttctctttct tcatcagtaa acaactcttt atacttttct
    18361 tttaatacta atacttctaa tgataaatct aatctcttac actcccatag tcttgtaaat
    18421 ccttcagtag ctccttcttt attgattaat tctttagcag ctaataaggc tcctttgtta
    18481 tttatcagtt gtaaaaatct tgttggctta tagttacatt gtaaatcagc ttctttatag
    18541 atatttacca tgttatggta taaatctagc tctatttgat tcatagattt tattccccca
    18601 ttttaataat tgtattttag ttgaataact ctcctttaaa agctttttgc attaatgagt
    18661 taaagttgtc ttctagttct tttaagctag tttccatttc aaacttcaaa gagtttactt
    18721 gtttaacgaa gttggcaaat tgattttgaa gtctaatagg aggtacaata atatttattt
    18781 ttctaacagc atctttagtt atcgtttttg ttacagctcc tcttactttc tcttttattt
    18841 tactttccat ataggatgtt agtaaaaggt tatataagaa tatatgattc actttatctt
    18901 cattaaatct aagcataaat tgattcatag ctaaagatac tggtttattt aaaaatggca
    18961 ttaaataaac tttacctgca ctacctatct tatttattat aacctcacca ccaaacacct
    19021 ttgatttttc aagataatta tatgcatgtt catcgatata cttaacacct ttttcaaagt
    19081 tattattttc taaatctgta gttcttacca ttaaggcata accttttgta tctaaaagag
    19141 ttacattatc tcttaaggtt tcataactac catttgaatg ataatcagtt aaaacatcta
    19201 aatatttccc tatttctgaa atttcccagt ttttttcatt ctttgatgga gtaccaaaca
    19261 tctcaataaa tcttgattta actaattcat ccaatacttc tatttgctct tttcttttat
    19321 ctattaactc ttgagccttg tctaaaacac gcactacctt ttcttgcact tttaaattat
    19381 acatgcgtat ttttatatta gctaatcctt cattatttaa agctgattgt gtagcacctt
    19441 tacttaacct atttttttga tttagaaaat aactactatt taagtaatgg tataaatatt
    19501 gaggcaatat ttcttttgat ggttttaaaa cataaaatcc agtcgatact attatatttt
    19561 tattagtttt attaataact aatgttttct ttgtgtcctt catttttgca aatataactt
    19621 ctcctatatt tacagtttga tttgctctag aaggtttatt ttcataagta actatttcta
    19681 gattgcttat cttgttaaaa tctaagtttc cagtactaag atatgagcgt tctccatcaa
    19741 acttttttac attttccccg cttatactaa caacatctaa aatttttgct tcatcaacca
    19801 tactctacac ctctaacatt ctttctagtt cctctaatcc atccactatc tctttctcta
    19861 acgccttaac cctacccata ataacactag gagcatcata aactacttct tcatacacta
    19921 cctctttata cttattaata gataaatcat acccattctc tcttatctct tccacatcaa
    19981 caaaaaaact ttgctctgtt ctttttctat ctacttcacc atcaagatta tcaaatctct
    20041 taataatatc ctctatatca ctatccttta tctcatttct cttatcatcc aaagaataac
    20101 catcagcttt catatcatag aaccatacct tatcagttcc acctgttcct gttttagtaa
    20161 atatcattat agctgtagat actcctgcat aaggcttaaa cactccactt ggcattgata
    20221 ttatagcttc caatttatga ttatcaacta tttcttttct tatcgattta tgtccacctg
    20281 ttgaaccaaa caatactcca tctggcacta tacatgcgca tcttccacca atttttaaaa
    20341 ctcttaaaaa aagtgccaag aataataact ctgttttctt tgtcttagtc acttttaata
    20401 aatctgctga cactgcttca taatctaaac ttcccttaaa aggaggattt gctaacacca
    20461 atgtaaactt atctttatct gtattaacct ccgataaaga atccttatac tctatattag
    20521 gattgtcaac gccatgcaac atcatattca tagcacctat tctaagcata gttctatcca
    20581 tatcaaatcc atagaacata ccattattaa aatgctcctt cagcccttgc actaagaaaa
    20641 gacttgaatg attatccctt aagtattgtt gtgatgatac taaaaaacct gctgaacctg
    20701 ctgccgggtc tactataata tcttctggtg ttggtttaat aagtcttact atcatatcta
    20761 ttatgtgtct tggagtccta aattgaccat tagtacctgc tgtagccact ttggataata
    20821 aatactcata caaatctccc tttgtatctc tatccttact taattctaaa ttactaatac
    20881 catcaactat tttagataac ataagaggcg taggaatttt aaatattgca tctcccatgt
    20941 actttgcata agctgagtct ttatttccat gtaatttttt tatgaaagga aacacttcat
    21001 tttgtactat ttcatacatt ttttctgaat ttccttcatt agaaaactta ctccatctta
    21061 aatgttgatg ttcatcatta aatatccttt caaaagaaac tcccaaaaga gttgcttctg
    21121 cttcattttt tgtttctact tcatccaatc ctttaataaa taaaagatat gtaaattgtt
    21181 ctataacttc taatggatta gttaatccac cagtccaaaa tgtctcccaa atcttgtcta
    21241 ctttgctcct taattctcct gtaatcatat atgactttcc ctccagtgtt cataaaataa
    21301 tttaaatata tattttagtc gtacgcctta atagttagtg tcttatgtta aatccaagtt
    21361 tttactacac agttattaaa ttatttttcc tatttacatg ttttagttaa aaaactaatg
    21421 caatactctt attttatgat tattatatat accatacaaa attattttat catatatcca
    21481 tcatagaagt catctacctg gataattctt accatattat aactttttaa tattaattta
    21541 aacttagaaa gaactttttt atttaattat atcatcttcc ttatttcaaa tttctcaatt
    21601 acaatattaa tcaaaaaata aaataagatg ctgtctcaaa atagatataa tcttcttttt
    21661 tattgtcaga atgatgaaaa tatatttaat ttcatacaaa aaagagtgcc tcatgaacta
    21721 aaaagttcat tttgagacac cccttaattt aatcttactt attctttccc tttattatta
    21781 acacttcact gtctctattg cttttcctac acatccacaa acttccatcc tactcttaac
    21841 aaaatcttta actgactcta aacctttttt accatatttc ttagggtcaa ttacctctgg
    21901 gtcagaatta agcacattct taactccatt gctataagca atccttaatt cagttgcata
    21961 attaaccttg caaatccctc tttttataga ttccttaata acttcatctg gtattccaga
    22021 cccaccatgt aatactaatg gaactgatac aacatctcta atttcagata atctatctaa
    22081 atcaagcttt ggctctccct tatataatcc atgtgccgta cctattgcta ctgctaaaga
    22141 tgatacccct gttctctcta caaactcttt tgcttctaat gggtctgtat atgctccccc
    22201 atcaccacca tctaaatcgt cttccttacc tccaacttta cctaattcag cttcaacagg
    22261 tatatctgaa ggcatacatg catctacaac agatttagat attgaaatat tatcttcata
    22321 actttcatga gaaccatcaa tcataataga agtatatcca actctaagtg cctgcatagc
    22381 tagtccaaag cttgaaccat ggtctaagtg cattgccaca ggaacacttg ccttctctga
    22441 tgctaccttt acattcgcta aataataatc tagtcctgca tactttattg ttgaaggtgt
    22501 tgtttgcaat attactggtg atttaagttc ttcagcagcc tctattactg ccataaccat
    22561 ctccatattt tcaacattaa atgcacctac tgcataatgc ccttcttgtg catctaataa
    22621 tatttgttta gttgtcacta atgccataat ttctcctcct atatgtttat actgatttaa
    22681 tttcttcgtt atttaatttt gatactattt ctctacttgt tttgctattt aataactcat
    22741 ttaaaaattc ttcattcatt aattttgaag atagttgaga taatgttttt aagtgtaaat
    22801 tatcaccatt atctgtagct gctatcataa atataatatg tgccaagctt ccatcttctg
    22861 attcaaagtc aaacccttct tttgatacac ctactgctac acgtggtacc tttactgcat
    22921 ctgtttttgc atgtggtatc gctatcccca taccaaaacc tgtagatgat atttcttctc
    22981 ttttaaggat ttcttcttta aacttctttt tatctaaaag aacaccagat ttatcaagta
    23041 ttccaatcat ttcatcaatt acatctgttt tattttttcc cttaacatct aaaattactg
    23101 tttcttcgct atataaagag atattattag atacgttttc cgattctgca ctttcatttt
    23161 tatgttcatc ttttgttgca tttgtatttt ctatagtatt tttctcattt actactttag
    23221 gtgtaaccat catataaagt acagcaccta ctaatgaacc tacaaatata caccctaccc
    23281 ataaaagtgg cttattgact aatcctagaa ccaaaaaccc tccatgtgga gctggtactt
    23341 ctattttcat tatataagtt aaaattgatg atattgatga tgcaatcata agtactggca
    23401 acacttttaa tggatttttt gctgcaaaag gtattgcccc ctctgttata tgagtactac
    23461 ctagtatata attgattaat cctgctgctc tatcttcttc tgtaaatcta tttttaaata
    23521 aagtagatgc tattgctatt accaatggtg gtgttataca agctgcagat acacctgcca
    23581 taaataaata atttccctgt cctagaagca tagttccagt tacatatgct gctttattta
    23641 ctggacctcc catatcaaat gctgacatac atcctattac tattcccaaa attataggac
    23701 ttgaattttg taaccccgat agaaagttca tcatagcatt gttaagtgct gaacatggcc
    23761 caccaagtaa caacattgct acacccacaa gacctacgct gacaatagga attataaaaa
    23821 tcgatttaag accttctaat tgtcgtggta attttcttaa caaatgtaca aatagtttaa
    23881 ctaagtaacc tgctaaaaat cctgatatga ttccacctaa aaaccccgaa cctgtatcaa
    23941 atgatatcat tcctccaaca agtcctgcca acataccagc tcttcccgat atagattgag
    24001 ctataaatgc tgccaataca ggaaccatca agttcataga atatccacca acagatttta
    24061 atgtagctgc tatttgatta tattgttctg aatttgggtc aaatgaataa atcccccata
    24121 aaaatgatat agcagtaagt actccacctg ctactactaa aggtaacatg tgagatacac
    24181 cattcattaa gtctttatac aacttttgac caaacgataa atttccttct tctgcaattt
    24241 tactgcttga taagtttcct tttctaatag ctacttttcc atcaatacat ctttgtataa
    24301 gttcattagc tttatgtata ccttctttga caggtacttc tattactggc tttccattga
    24361 atctgtccat atcaacattt ttatcacaag caactattat tgcatctgcc atttcgatat
    24421 cttttgctgt taacttattt tctacaccta tagctccatt cgtttcaact tttatgtcac
    24481 accctaattc cctggctgaa ttttttaatg cttcttctgc cataaaagta tgagctattc
    24541 cagttggaca acctgttacc cctagaatca ttttctttcc catttttata ccacctttct
    24601 gactcttata ttttttatat aatcttccac attctccaaa ttacctatac cattactttc
    24661 agccacactt gctcctgttg ctgctgacct ttttaatgct tcttcaatat gttctggatt
    24721 ttccaaccat atacttaaaa aagcagctaa tgcagaatca ccagcacatg cagagctgac
    24781 tactgtaact ggttgagcac tagcataata aattgatttt ccattataaa aataagaacc
    24841 tttttctcct aaagttagca atatattttg agcacctttt aaatgtagta atttaagtac
    24901 atccttaata tcttcttcat ctcttacaat aattccaaat atatctttga tttcttcatc
    24961 atttggtttt atcaaatatg gattatactg tagtaactcc tttaattttg gtgaacttat
    25021 atcgagtatt gtctttatac ttttattttt acaaatttta aatatatcct catagtaatt
    25081 atcatcaata ccaggaggta gacttccact tattgataag taacttatat cttctaatga
    25141 ctgtatctta tctagcatat ctagtttttg tttttcattt acatatgacc cagagtttac
    25201 aaatttaaat tcatcacttc catcatttaa aaatatattt atacgagttg tatcttctac
    25261 ccaagtaggc aatacgtcaa atcctctctt ttcgctttct tcaactatat attttcctga
    25321 aaatcctcca aaaaatccta atatcttaga ttctatacca taatgaccta gtacaaaagt
    25381 tacatttaat ccctttccat taggagaata tacagcattt gatgtacgat ttactaattt
    25441 tctctttatc ccattagtag atatattcat atctattgca ggattcgtag ttaatgtata
    25501 tatcattgtc ttcccccttg ctcgtgctgt gtatgttttc attataataa aaaagtctat
    25561 gaaaatcttt tcacaaatag aaaatcgatt ttcatagact tttcaattta attttcagaa
    25621 ttattatatc tgaccatata atctataatt atcctagata taatatacaa gggcaatctg
    25681 gaagatactt catattcaga aattgatata tgttcatgtt tgtatcctac aatatataaa
    25741 tccgaatact ttataagttt tgcactttca ctacaacagc aagttataac cttagctcca
    25801 actaaattgg cattctgtgc tgattctaca agctctatgg tatttccatt caatgaaaat
    25861 ataattataa gttcgtcttt ttttattgat ttagatttta ttttcattat gttggggtca
    25921 tttataaaaa caacattaaa atcaagtaat tgcaatttaa gactaaactc ttttgctaca
    25981 tattcagtaa gccctcttgc caaaatatat atcttactag atttatttat agtttttatt
    26041 atattgatta gtgtagttaa agatatttgt tctataactc tctgtgcttc tataaaggat
    26101 ttatttatta tatcccccat attttgtata tcatcatttt catcttttac tgttaatcta
    26161 tacctaagtt cattgaatcc atttaaacca cattttctaa ttgctcttga aacggttgct
    26221 ggagatgaat atgtttcatg agcaatctct actatagata attctggtaa tattgattca
    26281 ttttcattta taaatttaat tatttcaagt tccgtcttcg ttagattttc agaattactt
    26341 ttgtttagct caattatcat atgttatctc cccttatata agttttattt gtctaaattt
    26401 atcgagtaat ataatatatt acctcttaac tacttatttt tatatacaat taagatttag
    26461 tcaatgtcta tatatgtaaa tttataatat aataattttt tgcttaaagt ttattatgtt
    26521 atcttatagc attttaaaat aaaaaagaat tgtacctcta ccaaaatttg gatgaagtac
    26581 aatagatatt cctcatactg atatgtttat gattaaaagt ataaatataa atttcgctat
    26641 gaatcatata attaataaat cattttattt acaattataa ccccccttat aaaactatat
    26701 taataatata tacagtattt ataagggggg ttgtttatat ttaaaaacta tttacttttc
    26761 tcttaattct tctccattag ttcttattac attttcatac caataaaaac tatctttttt
    26821 ataacgttct ttacttccat ttccattgtt atctatatcc acatatataa atccatatct
    26881 tttttccatt tcttgagatg aacaacttac tatatctatt ggtgaccaca tacaataagc
    26941 taatatttcg gttccatcag ctatagcttc acctatttgt tctatatgtt ttcctaaata
    27001 atctattctg taactatcat gaacttttcc atcttcaaat ttatcatgca ttccaaatcc
    27061 attttcagct atcattattg gcttttggta tctatcataa tattgagata aagtattgta
    27121 taatcctttt gggtcaattg cccaccccca aggatttgct tttaaatatg gattctttat
    27181 attgtcagtt ggattcatgg tgtttttact tgctgatact gttgtagaat aatagtatga
    27241 taaagctaga aaatccatag tattttcttt taagatttta tcatcacctt cctccataac
    27301 tatatttatg ttattttctt tgaaaaatct cttaatatat actggatact ctcccctaaa
    27361 acttacatca gtgaaaaaat attgtagcct atttcttttt aaagcaaata ctacattttc
    27421 agggtcacaa tcttctggat aagatgtaca atctgccatc atcatcccta tcatcatatc
    27481 tgaatttatt tcttttccaa gcttcttagt caaagctgta gctatgaatt gatgatgaat
    27541 agcttgatat ctagcctctt ctacattatc tacttggtct acacaaaatg caactgaatt
    27601 aaatgattca taatgtatta aatttatttg atttataact atccaatatt taactttatt
    27661 tttatatcta tttagtatta attctgcata tctaacaaag aaatctacca cttttctatt
    27721 agaccatcct ttatatttta atactaaatt caaaggagtc tcatagtgaa gtagtgttac
    27781 aataggttcc ataccattat ctattacttc atttataaga ttatcataaa actttaatcc
    27841 ctcttcatta ggctctaaat catctccatt tggaaatatt ctagcccaat ttattgaaaa
    27901 tctaaaacac tttaacccta gcgctttcaa aagctttaaa tcttctttgt atgtatgata
    27961 aaagtctatt ccactacgct taggatagta cccattttta tctttcatag caaactttat
    28021 tctttctata gtcatatcag actcataagg tttagatata tctctatctt tatcatacat
    28081 atgtacatca gctaaacacc aacctttgcc accttcattc catgcacctt ctaattgatt
    28141 agctgctaca gctcctcccc ataaaaaacc tttaggaaac ttacccaatt taatcactcc
    28201 ttattttatt tatttaaatc atttactatt atttacctta tattctatag actttactat
    28261 tcctactaaa gacatagtta tatttaactc gctgaatatg gtcataagag tatcttgagc
    28321 atgtgcaaat aacagcgaat gtgctctttt ctctccacca gcttcccctt gtataatctt
    28381 ggtttgagct ctatgtgctt ctgtcataaa cccttttgct ttagctagtt ttaaatcagc
    28441 atttctataa tcaaactttt ccaaatcatt aagagcatgt ttaatttctg ttctagcctc
    28501 acctgcacga agtattattt gcattgccac ctctactaat tcttcatcta ttatatgttc
    28561 ttcaattgtc atatttataa tttcctttca atttctaaat ttaaacctct tggctctcta
    28621 attttatctc ttctttctca taagctttaa aaaatggata ccatatcata aatagcaata
    28681 ctatatttat tgctaaaaca ataagacctc taacatcttg atttaccata aaagtagata
    28741 taccaaaagg aacataccat aaattaaaca ccttacttgg aattgtcaca aatcccatac
    28801 tcattgataa gtaagtagtt attgatatta ctactgttcc tatccacata ggtaacatta
    28861 aaaatgggtt ccatgccact ggagctccaa atattactgg ttcatttata ttcattatag
    28921 aagggacaat acatgctctt cctattgctt ttattcttcc aactctggat aaacacatca
    28981 ttataactaa tggtaatgta gcacccaacc ctcctaaagt tatccaacct gtttgaataa
    29041 cttcgtaaac atatatatta gttgctactc ctcctgatgc tactgctgct gcattagctg
    29101 caattgcacc aagttgaatt gaataagtta taggagatag tacccaccca cttattccta
    29161 aagaatatat aaatactact aagaagttta agagtacaaa tccaggtaat gtctgtgcta
    29221 tatctgatac tggtttaaat actctaacta ttatttcaaa catgtcaaag ttgagtccat
    29281 aaattaatcc ccagcctaca aacattatca ctgttatagg taacatatta tcaaaccact
    29341 tgcttacaaa atccggaagt gctgaatctt ctccgaaaaa tgaaaaagaa ccaaataaag
    29401 acattattaa tccagagaat aatcctgcaa ctagcgatac aagcattcct tctcctccaa
    29461 acctgttgaa gttaaatata gctccttcat tcgtaatctc tggacgaatc atcattaaca
    29521 ttaatccagc acctgcaaga ccagctacta cactcatact ttcaaatttc ttattcttca
    29581 ttatttccat aggcaataaa aatgctataa ataatcctaa aagtccaaat gtatattgat
    29641 ttattggact taaatcaggt aaaaagctta tgtaattctt caagacagaa actatagtta
    29701 ctaatgagcc tacaaatacc attggcaaaa ctttcattat agttgattga actgattgta
    29761 cccatatatt cttagtcata ttatttacaa caggagtaaa tttttcactt aaaaaatcta
    29821 taaatttatt cataaacact ctccccataa atattttatt tgtttgatat tagttctagt
    29881 gcttcttcca aagcctttgc accatctagt cttccatatg cagttttaga tatacatgct
    29941 actggaacgt tatattcatt agccttttgt tgaatttctt ccttcatata atctaaatgt
    30001 ggtcctagta ataaacaatc tatttctgat aaatacttat ctatctctga ctcacttctc
    30061 gcttttatat ctgcttctat accttttttc tttgcactct ttctcatact agttgccata
    30121 aaacctgtac ttgctcctga accacacgcc aaaagcactc ttatattttt cataataata
    30181 cctccaatat ttttatccat taatttattt cttaatgaac tttttatttt attatgttta
    30241 tattgtactt accaaataag ttcatttcac ataaatatat ttcctaataa taggaaatca
    30301 gttactctta attaaacata ttaatatagt ataatgtatc ttataatacg tacattcaat
    30361 tatataatta tcatatcatc cagattttat tttaaaacta tgcgagggaa tctaatgaaa
    30421 acatctaaaa aagaactatt tttaaatttc ataaaaacac aacatgattg gatagattca
    30481 tctacattgg ctaattatct taatgtctca acgcgttcaa ttagaaaata tgtaaacgaa
    30541 attaattcca atggtgaatt tatattatcc tctaaaaaag gatataaagt aaatctcaat
    30601 aataattgtc aaaccaaggt tgatagttca gaaaatatat cacctgataa caggttaaat
    30661 ttaattttaa aagaacttat agttaatagt aatggaatta atatatttga tttaagtgaa
    30721 gaattgtttg ttagcccagc tactattgaa ggagatatag tcaaagctaa taaatttata
    30781 ggaagttata atttaaagat aaagcaatcg aaatttttat tgaaactaat tggaaatgag
    30841 tctgcaaagc gcaaattaat gagctctata atatttaaag aaacgggaag tgatttttta
    30901 agcttatttg atgtacaaaa aatatatcaa gagtataatt tga
//
LOCUS       DAELLV010000021        30455 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.021, whole genome shotgun sequence.
ACCESSION   DAELLV010000021 DAELLV010000000
VERSION     DAELLV010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30455
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.021"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(92..661)
                     /locus_tag="KPD58_000829"
     CDS             complement(92..661)
                     /locus_tag="KPD58_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG0264613.1"
                     /translation="MIIIYGYCRVSTKNQTDNNSLYQQQQEILNKYNDAKIIKETYTG
                     TTTNRPKFEKIISSLKKGDLLVATKLDRLARSTSEGIQLIEGLFAKEVSVHILNIGLL
                     ENTSMGKFFLTTLLAVAELERNMIIERTQLGKEFAKKKKDFKDGRPKKYSNQQIQYAL
                     GLLNSKPYREVVKMTGISKSTLIRAKKSI"
     gene            864..1091
                     /locus_tag="KPD58_000830"
     CDS             864..1091
                     /locus_tag="KPD58_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG0264614.1"
                     /translation="MNSLLKEAIDELKNIHCGEVFLLKELFKGYEWNRLEMNIRLKLG
                     KLFLYEVVTNPNLNVEVINKNSAYQQMYKKK"
     gene            complement(1310..2053)
                     /locus_tag="KPD58_000831"
     CDS             complement(1310..2053)
                     /locus_tag="KPD58_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBG0264615.1"
                     /translation="MENYQSTNTCTYNILYVQFNEDFERSKYFSYDVKYPVIYNIRNQ
                     STYVNPVILNNINNAVRMTVNNFKNGLQEEEREYNNATSQNSLPKQSYWVSTSYAVTF
                     NKNYVLSMILNLMGFANNYGPKYNTLNNYNIDLTTGKELLLNDIFRDGVDYINIITDY
                     IKSQINENREMYYSNVELVIPEDQAFYLTDNGIIIYFGVDEIAPESFGIPKFELKFSD
                     FGNFINLRFYCISPDIYVQSRGRAKHFMG"
     gene            2221..3093
                     /locus_tag="KPD58_000832"
     CDS             2221..3093
                     /locus_tag="KPD58_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG0264616.1"
                     /translation="MLLLADKWKDYELIDMGNGEKLERWGSYVLRRPDPQVVWPMESE
                     WALWKNPHGHYHRSNKGGGQWEHKKRYPEQWTINYKNLKFHIKPTGFKHTGLFPEQAA
                     NWDWMIEKIKKANRPIKVLNLFAYTGGATVACASAGAEVCHVDAAKGMVNWAKENIEL
                     SGLKNQTVRFIVDDVVKFVEREIRRGKKYDAIIMDPPSYGRGPKGEVWQIEEKLYSFV
                     DLCMGVLSDNPLFFLINSYTTGFSPIVLENVLSTTIGKKTNTGDIYGGELGIPASRDG
                     KILPCGIFGRWEKK"
     gene            3093..3758
                     /locus_tag="KPD58_000833"
     CDS             3093..3758
                     /locus_tag="KPD58_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBG0264617.1"
                     /translation="MIKVIYEDNHLLVVEKPVNILSQGDNTNDKDMVNLLKDYIKKKY
                     NKPGNVYIGLIHRLDRPVGGVMVFAKTSKAASRLSEQVRSKTFKKTYLAVVHGKIKSN
                     SDILKDFLYKNKKTNMVTVVKKSHKDAKEAELNYNVLDFKENLSLVQVNLKTGRSHQI
                     RVQFASRKHPLYGDQRYGKEINKIGQQISLWSNKIEICHPTTKEKMEFTCHPPDKYPW
                     NIF"
     gene            3779..4636
                     /locus_tag="KPD58_000834"
     CDS             3779..4636
                     /locus_tag="KPD58_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0264618.1"
                     /translation="MSKKFSIGEMSKLHKISIQTLRYYDQIDLFKPVHVDQESNYRYY
                     SIDQFSQLDIIKYLKYLGMPLKEIKSKLDENNEEILKLLTNKINIVDEKIKELELIKK
                     VLSTKKETMEKGMKVDEVGKIVRKHIPRRSILSIEYEEGLSSEETMELSRRQIANILE
                     DNISVFYGGVSGLVSIDKFLKEDKVIYDNSFVTVERDLFDIKSQKHITEISSGDFICM
                     TYKGPYKENYTAFKKLVDYVEYKRIPIENYIYEIPIIDPLSTNNEEELLTEIQIQVKK
                     REIDLRCLV"
     gene            complement(4792..7338)
                     /locus_tag="KPD58_000835"
     CDS             complement(4792..7338)
                     /locus_tag="KPD58_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG0264619.1"
                     /translation="MNIFNKVALQGLKKNRTRTLVTIIGVVLSAAMITAVTTFGVSLM
                     NYMTNGAIIKYGDWHVQFLDVDSSFVQEQRVNDEVANTATFENIGYATLEGNRNPDKP
                     YLFIAGFSKEAFDTLPITLISGRLPENSREILVSSKVATDGGVKFAVGDTISLDVGKR
                     MSENEKLGQSDPYTAGSEVFVPQDKKTYTVVGICRKPVFEESSAPGYTLITKEDKQVK
                     TDSLSLFVTLKNPHQIRSYISSTKDHASIFNDNVLRFIGVSDNNLFNMFLYSVGGIVL
                     AIIMLGSIFLIYNSFNMSLNERTHQFGILSSVGATAKQLRNSVLFEGICIGAIGIPIG
                     VAIGIVSIGLVISVIAKNFANILYVNVPLTLTLSIPAIIVAAVVSMVTILISAYIPAR
                     KAANTPVMECIRQTNEVKIESKSVKTSKLTQRVYGLEGVLAMKNFKRNKKRYRSIVLS
                     LVLSIVLFISTSALVADLKQMSNQRSEVTDQDIIFSTQGMKDNEMLKLYDKLKTANGV
                     YEGLYQKLMTYSCVAKVSELTDDYLNYEGSHSMSEMLELPVDMQFLDDRTYLNIIKSL
                     GLPAEEYTGQNAKLIAVSMVTKDNNIIDIFNSSSMNVSLTPETNGKLEIPERQNVNIS
                     FVKVVPPDSPPGIRSSDVKKASFRVLAPYSAKDKFEIPGVSTGIESLSFQSKNPTQSV
                     AEMEQIIESEGVTNEYNLYNMYGMMDENRNYIFIANVFAYTFIIMISLISIANVFNTI
                     STNIKLRRRELAMLRSVGMSEHDFQKMMNFECAFYGMRALFFGLPIAVISSWLIHKVM
                     VTDDIDFVIPWFSIGISIFSVLFIVFITMLYTINKIKKENIIDALRDDMA"
     gene            complement(7338..8024)
                     /locus_tag="KPD58_000836"
     CDS             complement(7338..8024)
                     /locus_tag="KPD58_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0264620.1"
                     /translation="MAFLKIEDLCKVYGKNENKVTALDHVSLTIEKGEFTAIIGSSGS
                     GKSTLLHSIAGVDVPTSGKIYLEGQDVYGQSNEKLAIFRRRQVGLIYQFHNLIPTLNV
                     VENITLPILMDKRKVNQERLNDLLELLGLKERKTHLPNQLSGGQQQRVAIGRALMNAP
                     AVMLADEPTGSLDSKNGQEIIQLLKESHSKYHQTLIIVTHDENIALQADRIICISDGK
                     VVRDERKVNR"
     gene            complement(8107..9096)
                     /locus_tag="KPD58_000837"
     CDS             complement(8107..9096)
                     /locus_tag="KPD58_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0264621.1"
                     /translation="MFRNREFRKFAILFLLITILTVALGFAISIMTGILSIVSATTFG
                     IAFFVFTKNRYKSIAQISEQINLVLHNANHLYIAESDEGELSILQSEITKMTLRIREQ
                     NYALKKEKEHLADSLADIAHQLRTPLTSVTLILSLLENTSDEDERKELIRETEELLIR
                     MDWLITSLLKLSRLDAGIVVFQKEQIDVNNLISSALHQLLIPMELHNITLHIDIPKGV
                     RILGDLNWLSEAIQNIFKNCMESVGDNGKIDIICEDNFLFTQLTIHDNGAGFKKEDLP
                     CLFNRFYRGKNSSTAGYGIGLALCKTIIMRQGGTITAQNHPQGGAIFVIRFPK"
     gene            complement(9083..9769)
                     /locus_tag="KPD58_000838"
     CDS             complement(9083..9769)
                     /locus_tag="KPD58_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0264622.1"
                     /translation="MKRIFLVEDDKAIAKNLILLLNSEGFIVTHVSTRSEALVEIAKN
                     KFDLALIDISLPDGNGFTICTEIKEKQDIPVIFLTASGDESSVVTGLNMGADDYITKP
                     FRPRELIARIRTALRKSGRFGSTFEICGLYVDTATGVVKKNGNEVFLSALEYRLLLVF
                     ISNPKSIITRVRLLDELWDAAGEFVNDNTLTVYIKRLREKIEDDPSSPQIILTVRGTG
                     YRLGGSYVSK"
     gene            complement(10269..13475)
                     /gene="carB"
                     /locus_tag="KPD58_000839"
     CDS             complement(10269..13475)
                     /gene="carB"
                     /locus_tag="KPD58_000839"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBG0264623.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQALKEEGIEV
                     VLVNSNPATIMTDKEIADKIYIEPLTIEFIEKIIEKEKPDSLLAGMGGQTGLNLAVEL
                     HDAGILDKYNVKVIGTSIESIKKGEDRDLFREVMKEINQPVIVSDIVTNLEAGLEFAN
                     KIGYPVVVRPAYTLGGTGGGIADTEEELREILSHGLQLSPVGQVLLEKSIKGWKEIEY
                     EVMRDGNGNCITVCNMENVDPVGVHTGDSIVVAPSQTLSDEEYQLLRKASIDIINAIE
                     VQGGCNVQIALNPHSLEYAIIEINPRVSRSSALASKATGYPIAKVAAKIALGYTLDEI
                     ENAVTKKTYACFEPTLDYVVVKIPKWPFDKFRHANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHAPSEGRTLEELKARVVVPDDERLFDLAEMIRRGYKVEMISE
                     ITGVDKWFINKFKWIVEQEEKLKVLKIEDLDKEYLHELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIQPVYKMVDTCGGEFEALSPYYYSTYEQYDEVVVSDKRKVVVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMGIETIIVNNNPETVSTDFDTSDKLYFEPLTEEEVLNI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFRDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWTTNEGVEIAKELGYPVLVRPSYVLGGQGMEITYNEEKLIQYLDDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGVPIV
                     DLATKCMLGAKLKDLGYSTGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEVL
                     GVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAKDMKELGYTFVATE
                     GTAKSLRENGIEADIVNRIEEPRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKMRRT
                     AIEFSTEIMTSLDTLKALVEVKKKHLNKDKLKVYNIAE"
     gene            complement(13668..14717)
                     /gene="carA"
                     /locus_tag="KPD58_000840"
     CDS             complement(13668..14717)
                     /gene="carA"
                     /locus_tag="KPD58_000840"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBG0264624.1"
                     /translation="MKARLILEDGTVFIGKAFGYLEESVGEVVFNTSMIGYGEVLTDP
                     SYYGQIVTMTYPLVGNYGINLSSAESEKVQVKGFIVREKSDSPSNFRCEIDIDQYLKQ
                     NKVIGLEGIDTRALTKILRNNGTMKGIITLEDSKLEDVKHKLDKFSNTEAVRTVTRKE
                     VEHIKGNGPKVAVMDFGVKRNILRSFIARGCDITIFPATTSPEDVLSINPDLIFLSNG
                     PGDPEDLEDVIENIKALIGKKPIVGICLGHQLLALALGGKTAKLKFGHRGGNHPVKDL
                     EEGKVFITSQNHGYYVSEVPEQMKVTHINLNDNTVEGMRHEKLDVYSVQYHPEACPGP
                     KDNDYIFDKFLELVK"
     gene            complement(14974..18180)
                     /gene="carB"
                     /locus_tag="KPD58_000841"
     CDS             complement(14974..18180)
                     /gene="carB"
                     /locus_tag="KPD58_000841"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBG0264625.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQSLKEEGIEV
                     VLINSNPATIMTDKEVADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     YEKGILDKYGVKIIGTSVESIKKGEDRDIFREVMKEINQPVIVSDIVTDLQAGLDYAL
                     TIGYPVVVRPAYTLGGTGGGIADNEEELREILSHGLQLSPVGQVLIEKSIKGWKEIEY
                     EVIRDSKGNCIVVCNMENIDPVGVHTGDSIVVAPTQTLSNKECAMLKKASLDILNAVE
                     VQGGCNVQFALNPHSFEYAVIEINPRVSRSSALASKATGYPIAKVSSKIALGYTLDEI
                     ENAVTKKTYACFEPTIDYVVAKIPKWPFDKFKKANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHTPSEERSIEELKKRVVVPDDERLFDLAEMIRRGYKVEMIEQ
                     ITGIDKWFINKFKWIVEQEEKLKVMKIEDLTKDYLLELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIKPAYKMVDTCAGEIDAISPYYYSTYEQYDEVVVSDKKKVIVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMDIETIIINNNPETVSTDFDTSDKLYFEPLTEEEVLSI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFDDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWSLNEGIEVANELGYPVLVRPSYVLGGQGMEITYNEEKLSQYLQDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGVPIV
                     DLATKCMLGAKLKDLGYGTGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEVL
                     GVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAKDMKELGYTFVATE
                     GTANSLRENGIEADIVNRVEESRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKIRRT
                     AIEFSTEIMTSLDTLKALVEVKKKHLNKDELKVYNIAE"
     gene            complement(18233..19276)
                     /gene="carA"
                     /locus_tag="KPD58_000842"
     CDS             complement(18233..19276)
                     /gene="carA"
                     /locus_tag="KPD58_000842"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBG0264626.1"
                     /translation="MKGKLILEDGTVFEGKAFGYLKEAIGEVVFNTSMAGYGELLTDA
                     TYYGQIVTMTYPLIGNYGINLEDVESNKVQVKGLIVREKSDNPNNFRCEMDIDTYLKQ
                     NKVIGLEGIDTRALTKIIRNNGTKRGIITLENVSLQDVQSKLEEHCNKDSVKNVTRKE
                     IENIKGTGAKVVVIDFGVKNSVLESLKACNCDITIVPAMTTVEEILNINPDLILLSNG
                     PGNPEDINDIVENIKKLIGKKPLTGIGLGHQILALALGGKTSKLVFGHRGGNHPVKCL
                     ETGKVFITSQNHGYNVSEMPENTEVTHINLNDNTIEGMRHKELPIYSVQYIPEYTTKE
                     DLDCIFNKFLALI"
     gene            complement(19302..20018)
                     /gene="pyrF"
                     /locus_tag="KPD58_000843"
     CDS             complement(19302..20018)
                     /gene="pyrF"
                     /locus_tag="KPD58_000843"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBG0264627.1"
                     /translation="MINGKEKLIVAIDTDEFDKAKELIDRLEDSVDIFKVGLEQYVAT
                     KGKTIDYLKEKGKKIFLDLKFHDIPNTMKSAVKAAVRDNVWLMTIHVSDMEGMRQCAL
                     IAREEAERLNIEKPLVVGVTVLTSLSNQDLQDIGCNMTTEELAIKRAKLAKESGIDGV
                     VCSAQEVDKIVEACGTDFITVCPGIRPKSAEVGDQKRVVTPSDAIKKGAHYLVVGRPI
                     TKAENPRESAINIVREIENA"
     gene            complement(20394..21029)
                     /locus_tag="KPD58_000844"
     CDS             complement(20394..21029)
                     /locus_tag="KPD58_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HBG0264628.1"
                     /translation="MYKCGKCNLRGCSNGNMDKLMKICPTREIELQQKAKQLYEEEEN
                     RKIAYNAGLVEAEGYCQYTRLKEIIEFAKKCGYKKIGIAFCIGLKREMQTVQKIFEHH
                     GFEVFSVICKNGAMPKSTIGLEASQTISKCSDEVMCNPIGQALFLNEAKVDLNVILGL
                     CVGHDTLAMKYMEAPITTLVVKDRVTGHNPVAAIYNAESYFEKKLFDKDEE"
     gene            complement(21053..21967)
                     /locus_tag="KPD58_000845"
     CDS             complement(21053..21967)
                     /locus_tag="KPD58_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264629.1"
                     /translation="MELYEAIFYRKSVRNFSSKKIKKPLMEEIKRSCKNLEYLNEDLN
                     IKAHVVDRGHIIHFLMGKECKVKAPHYIVVTSDKGEDYLQNIGFATEGVVLRLTTLGI
                     ATCWLEGNLKREDILEFVDIGNKNFEEDEEDDLDYLAGIKTEEDDENKIENPYIIIAF
                     GYPAKNEELFRTRYARPDRKPVKDMINKINRNRKWKDILELTRRAPSVKNSQPWYFHK
                     DERGLHLFEKRPKKHCEDMNKVSLGVALRHFDIACIKNKIDVSYEKLPIRNKIGKSYF
                     ITVVEHVKPEEETQEENVTLEKEESQDE"
     gene            22568..23797
                     /locus_tag="KPD58_000846"
     CDS             22568..23797
                     /locus_tag="KPD58_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBG0264630.1"
                     /translation="MNLNRNLEKYAELAIKVGVNIQPGQILLIKSPIECADFARNALK
                     EAYKCGAKNVYIEWSDEESTLIKYLYAPDEAFHEFPKWTAEQYVDIAKEGGAFLSVYA
                     QNPDLLKDVDPERVANFQKASGKALKEWRSYTLTDKCKWSIVSVPTKDWAKKVFPNLS
                     EDKAIEKLWDAIFKCSRVDGQDPIKAWEEHNENLKSKMDFLNKNNFKTLKYKSSKTDL
                     TLDLPKGHVWLSGASKDPNGISFNPNIPTEEIFGMPHKFKVNGTVYSTKPLVYGGNII
                     DNFFLTFKDGKIIDFSAEKGLDTLEKLIETDEGSHYLGEVALVPYNSPISNTDIIFYN
                     TLYDENASCHFAIGSAYKTCLEGGNNLKDEELDEHGVNDSLTHVDFMIGSADMDIIGE
                     TYDGKQIQIFKNGNWAF"
     gene            complement(24002..26383)
                     /locus_tag="KPD58_000847"
     CDS             complement(24002..26383)
                     /locus_tag="KPD58_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HBG0264631.1"
                     /translation="MEIRQYLKNNILIFDGAMGTMLQQKGLKLGENPEVFGLQNPDKL
                     IEIHTAYLEAGSNVILTNTFGCNELKLDSKYTVEEVIDNAVLVARKAIENVDNTKPRY
                     VALDIGPIGEMLEPMGTLSFDKAYEIFKRQVLQGVKSGVDVIVIETMMDLYEAKVAVL
                     AAKENSDLPIFCTMTFDEGGRSFTGCMPECMVATIEGLGVDAIGVNCSLGPKQLLPIV
                     EKIASRATVPVMVQANAGLPNIVDGEAIYDVDAKEFFEGVKKFVEVGATIIGGCCGTN
                     PSFIKEISENINSVTKGCIEKIDKCVVCSPSKFVEVQSPTVVGERLNPTGRKSLQEAL
                     KNENVDYAINLGLEQVNAGAQILGVNVGLPEIDEKKLMPKLIREIQAVVDTPLQVDSS
                     NVEALEQGLRYYNGRTIVNSVNGKEESLESILPIVKKYGSCVVGLTLDEKGIPKKAEE
                     RFEIAKRIVNRAVSYGIKPKDIFIDCLSLTVSAQQEEAIETIKAITMVKTLGVKTILG
                     VSNISFGLPNRKALNASFLTLALGAGLDLAIINPNEYSMMEAINSFKILNNTDKGCIN
                     YINQYSNINNSKKSDSSTKIDKDLPLDILVERGLKDEAKNVTLNLLKEKDENYILDEI
                     LIPALDKVGKRYDSGDIFLPQLIQSAETVKVSLNTIKETLLSKSSNNVSKGKIIVATV
                     KGDIHDIGKNIVKIMLENYGYEVIDLGKDVPIEEVVEVAKKRNIKLVGLSALMTTTVQ
                     SMKDTIQALRDNEINAKVFVGGAVLTEEYAEEMGADYYSKDAKSAVEIAKLNF"
     gene            complement(26355..27080)
                     /locus_tag="KPD58_000848"
     CDS             complement(26355..27080)
                     /locus_tag="KPD58_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vitamin B12 dependent methionine synthase,
                     activation domain protein"
                     /protein_id="HBG0264632.1"
                     /translation="MENNLIGREDISINQSEVLRYLQYRGQEIDKDLKYTIDECVAIT
                     KNKINPRYLSRIYPIKINKDNVVELEGTNLTLESKDVYELLKECNECVIMAATIGIDI
                     EKEIRRHSYSNLTKGLIIDSCGTTAIEEVCDIVQNNLEKVFLKQDKFITMRYSPGYGD
                     LPIEKNIDILNVLNAQKQIGLTITSNGIMIPRKSVVAIIGISNTRINKSKKEKSCENC
                     KNYRNCTYRKGANSCGNKTISEK"
     gene            complement(27070..27525)
                     /locus_tag="KPD58_000849"
     CDS             complement(27070..27525)
                     /locus_tag="KPD58_000849"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBG0264633.1"
                     /translation="MEKVESFKLDHTKVKAPFVRKCSVLDGVKGDKVTKFDLRFLQPN
                     VESFGTAAMHGLEHLLATYLRDTLDGVIDLSPMGCRTGFYLILWGDVDAKTVKIGLEE
                     ALKKVLESDKMPAATAIECGNYRDLSLFGAKEYAKDVLDKGFSLNIYGE"
     gene            complement(27807..28988)
                     /locus_tag="KPD58_000850"
     CDS             complement(27807..28988)
                     /locus_tag="KPD58_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG0264634.1"
                     /translation="MKNKSLLNHMFRPILDLIISCALTSMLILTSMYIIKSLYVNSDM
                     SVVNQLVYQARDFRSLIGYDLFYPFLFTITSLAIYSMISYKRNKQIVDFIRKTNRKNQ
                     KLEIIKSAVNLMDEGNLEKSIDIEYDLDILKDKEKDDIDELAHKINNIVHQLRNITIE
                     ERQAQQTKTDLITNVSHDLRTPLTSIMGYLGVIEEGKYKDEVQMMYYVDIAYEKSKNL
                     NVLINDLFELTKMQNNTIKLNKIEINLVELLSQVVSQFEIYFKQEFMVSRINFNEEKL
                     IVKADPNKLVRAFENLITNAVKYGKDGHYVDIVTEKKEDMAVVKVINYGEPIPVLDLP
                     NIFDRFYRVEKSRNRNVGGSGLGLAITKNIVNLHDGEITVSSDKYQTVFEVQLPIIQS
                     A"
     gene            complement(28978..29670)
                     /locus_tag="KPD58_000851"
     CDS             complement(28978..29670)
                     /locus_tag="KPD58_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0264635.1"
                     /translation="MNNTVLVVDDEKEIRDLIEIYLLNSGYNVIKAEDGKEALDILKK
                     ENIHLMILDIMMPKMDGIEVCRKVRESLNIPILMLSAKSEDMDKIQGIMTGADDYLTK
                     PFNPLELTVRVKSLIRRAYYFSGANNEDKDVIKVGVVIIDKEKHSVTVAGNEIILTSR
                     EFDILYLLANNKGRVYSTEEIFEKVWKEKYYQSNNTVMVHMSRIRDKIEKYTDGEKII
                     HTVWGVGYKIEK"
     gene            complement(29804..>30455)
                     /locus_tag="KPD58_000852"
     CDS             complement(29804..>30455)
                     /locus_tag="KPD58_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG0264636.1"
                     /translation="DRLLKKDKDPIVAAQHDSEQDVEANSQNKNHSNSISNILLVNKT
                     NGISKNYTPENITKVNIPFVEEATEEEKQMAGEPAKAVEDLVKQANSEGIQFLGSSAY
                     RSYDTQLDTYTRRVKSQGREKADAYVAKPGYSEHQTGLCIDLTNPERWFVGSTKEAKW
                     LAENAHKFGFIIRYPEGKEDITGTAYEPWHIRYVGKDAAEEIYSKGLTLEEYLQNK"
BASE COUNT         9621 a         5511 c         3671 g        11652 t
ORIGIN      
        1 tttctattcg ctacatctaa aaataattag atagaggtat aaatgtattt agtttattta
       61 tacctctagt cttttataaa ttatatctct actaaattga ttttttagca cgaataagtg
      121 tgctcttgga gataccagtc atcttcacaa cctctctata tggcttagaa tttaataacc
      181 ccaatgcata ttgtatctgt tgattgctgt atttctttgg cctaccatct ttaaagtcct
      241 ttttcttttt agcaaattct tttcctaatt gagtacgttc tattatcata tttctctcaa
      301 gttctgcaac tgcaagtaat gtagtcaaaa agaatttacc cattgaagta ttttctaaaa
      361 ggcctatatt taaaatatga acacttactt ctttagcaaa taaaccttct attagttgta
      421 ttccttctga tgtacttcta gctagtctat ccagcttagt tgcaacaagt aagtctccct
      481 tttttagaga tgatataatt ttttcaaatt taggcctgtt tgtagtagtg cctgtataag
      541 tttcctttat tattttagca tcattatatt tattcaaaat ttcttgctgt tgctgataca
      601 aactgttgtt gtctgtttga ttttttgtac ttacacggca atatccatat ataataatca
      661 tcacttccta acataaagat tttgtacaga agttacccag aagcttgttt ttacttatct
      721 ttatccattt tactaaaacg atttttgggt gtcaatactg ttaacttttg acaccataat
      781 aattccttta taatttgttt gactatgtca aaaattacca atataatgaa attaatgagt
      841 ttatacaaat tggaggaaat aatatgaata gtctgttaaa agaagcaata gatgaattaa
      901 aaaacatcca ttgcggggaa gtttttttat taaaagaatt gttcaaaggc tatgaatgga
      961 acaggttgga gatgaatatc agattgaaat taggaaaact ttttttgtat gaagtggtaa
     1021 ctaatcccaa tttaaatgta gaggtcatca ataaaaattc tgcttatcag caaatgtata
     1081 aaaaaaaata gtgacttaaa tgtatataat agtataatct tcttattgat tcaaaagtta
     1141 aaacttttaa tacaaaacaa tcattctatt ttaacattta tcaatatgtc taaatttata
     1201 taattaacac atacgaatag tattgtttaa atcacattat ttaagataga taaatcttaa
     1261 ccctactcca gcataaatac taaaataggg cttagaatta tctatatttt tatcccataa
     1321 aatgcttagc tcttcctctt gattgaacat atatatctgg ggaaatacaa tagaagcgta
     1381 aatttataaa atttccaaag tcagaaaact tcagttcaaa cttaggtata ccaaaacttt
     1441 caggtgctat ttcgtcaacg ccaaaataga tgataattcc attatctgta agataaaatg
     1501 cttggtcttc aggtataact aactcaacat tagaataata catttctcta ttttcattta
     1561 tttgagattt aatgtaatca gtaattatat taatataatc aactccatct ctaaaaatgt
     1621 cattaagtaa aagttctttt ccagttgtca aatctatatt gtaattattc aatgtattgt
     1681 atttaggccc atagttatta gcaaacccca taaggtttaa tatcatactc agtacataat
     1741 ttttattaaa agtaactgcg taactggtag atacccaata gctttgtttt ggaagtgaat
     1801 tttgagaagt tgcgttatta tattctcgtt cttcttcctg taagccattt ttaaaattat
     1861 ttacagtcat tcttacagca ttgtttatgt tatttaaaat aactgggttt acatacgtgg
     1921 attgatttct aatattgtat ataacaggat actttacatc atatgaaaaa tacttacttc
     1981 tttcaaaatc ttcattaaat tgtacatata atatattata tgtacaggta tttgtagatt
     2041 gataattttc cattaaaatc ctcccccttt tataaaaaaa tcagatacaa tataatatgc
     2101 ttaaaacagt aataatgtta atggtaaatg taaagaatag aaagtatcta aaacagcaaa
     2161 aaatgactaa ttttgtggta taatacatag gattgagagt tattttatag gaggaaaaat
     2221 atgttattat tagcagataa atggaaagat tatgaactta tagatatggg aaatggagaa
     2281 aaattagaac gttggggaag ttatgtactt agaagaccag acccacaggt tgtatggcca
     2341 atggaatctg aatgggcttt atggaaaaac cctcatggac attatcacag aagcaataaa
     2401 ggtggaggtc aatgggaaca caaaaaaaga tatccagaac aatggactat aaattataaa
     2461 aacttaaagt ttcacattaa accaactggc tttaaacata ctggattatt cccagagcaa
     2521 gcagctaact gggattggat gatagagaaa ataaagaaag ctaacagacc tataaaagtg
     2581 ttgaatcttt ttgcatatac aggtggagct actgtagcat gtgcatctgc tggtgcagaa
     2641 gtatgtcatg ttgatgctgc aaaaggtatg gttaattggg caaaagaaaa tatagaatta
     2701 tcaggtctta aaaatcaaac agtaagattt atagtggatg atgttgtaaa gtttgttgaa
     2761 agagaaataa gaagaggtaa aaaatatgat gcaataataa tggaccctcc ttcttatgga
     2821 agaggtccta aaggagaagt atggcaaata gaagaaaagt tatacagttt tgtagattta
     2881 tgtatgggag ttttatcaga taacccttta tttttcttaa ttaattctta tacaacagga
     2941 ttttctccaa tagttcttga aaatgtattg tctacaacaa taggtaaaaa gacaaataca
     3001 ggagatatat acggtgggga attaggaata ccagcttcca gagatggtaa gatacttcct
     3061 tgtggtatat ttggaagatg ggagaaaaaa taatgataaa agtcatatat gaagataacc
     3121 atttattagt tgtagaaaaa ccagttaaca tattgtctca aggagataat actaatgata
     3181 aagatatggt aaatctttta aaagactata ttaaaaaaaa gtataacaaa cctggaaatg
     3241 tatatattgg tttaatacat agattggata gaccagtagg tggagttatg gtgtttgcaa
     3301 aaacatcaaa agcagcatct agattgtctg agcaggtaag aagtaagact ttcaaaaaaa
     3361 cttatctagc tgttgttcat ggcaagataa aatctaactc agatatacta aaagattttt
     3421 tatataaaaa taaaaaaact aatatggtaa ctgtagtgaa aaaaagccat aaagatgcca
     3481 aagaagctga acttaactat aatgtattag attttaaaga aaatctaagt ttagttcaag
     3541 ttaatttaaa gactggtcgt tctcatcaaa taagagttca atttgcaagc agaaaacatc
     3601 cattatacgg agaccaaaga tatggcaaag aaataaataa gattggacaa caaatatctc
     3661 tttggtccaa taagatagaa atttgccatc ctactacaaa agaaaaaatg gaatttacat
     3721 gtcatccacc tgacaaatat ccatggaata tattttaaat ctcaaatgag gtgattatat
     3781 gagtaaaaaa ttttcaatag gagaaatgtc taaacttcat aaaataagca tacagacact
     3841 aaggtactat gaccaaatag atttatttaa acctgttcat gttgaccaag aaagcaatta
     3901 cagatattac tcaattgacc aattttcaca attagatatt ataaaatatt taaaatattt
     3961 aggtatgcct ttaaaagaaa ttaagtcaaa gcttgatgaa aataatgaag aaattctaaa
     4021 attactaaca aataaaatta atatagtgga tgaaaaaatt aaagaattag agcttataaa
     4081 gaaggtacta agcacaaaga aagaaactat ggaaaaaggt atgaaagtag atgaagttgg
     4141 taaaatagtg agaaaacata ttccacgaag aagtatctta tctatagagt atgaagaagg
     4201 attaagtagt gaagaaacta tggaactttc cagaagacaa atagcaaata tattagaaga
     4261 taatatatct gtgttctatg gtggagtaag tggacttgta tctatagata aatttttaaa
     4321 agaagataaa gttatttatg acaatagttt tgttactgtt gaaagagact tatttgatat
     4381 aaaatcacaa aaacatataa cagaaatttc atctggtgat tttatttgta tgacatataa
     4441 agggccttac aaagaaaatt ataccgcttt taagaaactg gttgattatg ttgaatataa
     4501 aagaatacct attgaaaatt atatatatga aatacctata atagaccctc tgtctacaaa
     4561 caacgaagag gaacttctaa cagaaattca aatacaagtt aaaaagagag aaattgactt
     4621 aagatgtcta gtgtagtaat ctagtttact tacacatctt aatatagttg tctattatca
     4681 acatatgatt tttctagata gtgattccta acagtgagat tttcaaaaaa taggaggaaa
     4741 tatggctaat gctaattccc tcctattttt aattaagcca tatcatttta attaagccat
     4801 gtcatcacga agtgcatcaa taatattttc tttttttatc ttattaatag tatacaacat
     4861 tgtaataaat actataaata aaacactaaa tatactaatt ccaatactaa accatggaat
     4921 cacaaaatca atatcatctg taaccattac tttatgaatt agccatgaag aaattactgc
     4981 tataggaagt ccaaagaaca atgctctcat gccataaaag gcacactcaa aattcatcat
     5041 cttttggaaa tcatgctcag acattcctac agagcgaagc atggcaagtt ctctacgacg
     5101 taacttaata ttagttgaaa ttgtattaaa cacattggca atcgaaatca gagaaatcat
     5161 aattatgaaa gtataagcaa aaacattggc aataaaaatg taattacggt tctcatccat
     5221 cattccatac atattgtaca gattgtactc attcgtaact ccctcactct caataatttg
     5281 ctccatttct gcaactgact gagtaggatt tttcgattga aagcttaaac tttcaattcc
     5341 tgtagaaaca ccaggaatct caaacttatc tttggctgaa taaggagcca atactctgaa
     5401 gcttgctttc ttcacatctg aacttctgat tcctggagga gaatcaggtg gcactacttt
     5461 gacaaatgaa atattcacat tttgtctctc aggtatctcc aacttgccat tagtttcagg
     5521 agtgaggcta acattcatag aagaactgtt gaaaatatca ataatattat tatcctttgt
     5581 taccatagaa acagcaatca gttttgcatt ttgtccagta tattcttctg ctggtaatcc
     5641 aagactttta ataatattca gataagttct atcatcaaga aattgcatat ctactggtaa
     5701 ttcaagcatt tcactcatag aatgtgaacc ctcatagttc aaataatcgt ctgtaagctc
     5761 acttactttg gcaacacatg aataggtcat aagtttctga tacaaacctt cgtaaacacc
     5821 atttgcagtt tttagcttat cgtaaagctt caacatttcg ttgtctttca taccttgtgt
     5881 agaaaaaata atatcctggt cagtaacctc tgacctctga tttgacatct gttttaggtc
     5941 tgctactaaa gcacttgtag atataaatag aactatgctt aaaacaagcg acaatacaat
     6001 acttcgataa cgtttcttgt ttcttttaaa gtttttcata gcaagaactc cctcaagacc
     6061 ataaactcgc tgtgttagtt ttgatgtctt cacagattta gattcaattt ttacctcatt
     6121 tgtctgacga atacactcca tcactggtgt gttggcagcc tttcttgctg gtatgtaagc
     6181 agaaattaag attgtaacca tacttaccac tgctgcaacg atgattgcag ggatagacaa
     6241 tgttaatgtc aacggaacgt ttacatagag aatatttgca aagtttttag ctatgacaga
     6301 aatcacaagt ccaatactga ctataccgat tgcaacacca attggtatac caatcgcacc
     6361 aatgcaaatt ccttcaaata gcaccgaatt tcgtaactgt tttgctgtag ctccaacaga
     6421 tgaaagtatt ccaaattgat gtgttcgctc gttcaatgac atgttaaatg aattatatat
     6481 tagaaaaatt gagcctagca tgattatggc aagcacaatg ccaccaaccg agtacaaaaa
     6541 catattgaac aaattgttat cagaaacacc tatgaagcgc aacacattgt cattgaaaat
     6601 ggaagcatgg tctttagtgc tacttatgta agagcgaatc tgatgtggat ttttcaatgt
     6661 gacaaaaagg ctaagactgt cagtcttgac ttgtttatct tctttggtta tcaaggtata
     6721 tcctggtgca gaagattctt caaatacggg ctttctacag atacccacaa ctgtataagt
     6781 tttcttatcc tgtggcacaa aaacttcact cccagcagtg tatgggtcac tctgaccaag
     6841 cttttcattt tcactcattc gtttccctac atccaaagaa atagtatcac ctactgcaaa
     6901 tttgacacca ccatctgtcg caactttact tgaaacaaga atctctctac tattttcagg
     6961 taatcttcca gaaattaaag ttataggcaa agtatcaaaa gcttctttgc taaatccagc
     7021 aataaaaaga tatggtttat ctggatttct gttaccttca agtgttgcat agccaatatt
     7081 ttcaaatgtt gcagtatttg caacttcatc attgactctt tgttcctgta caaaagaaga
     7141 atccacatcc aaaaactgga catgccaatc accatatttt ataatagctc catttgtcat
     7201 atagttcatc aaagaaactc caaaggtagt aactgctgta atcatagcag ctgacagaac
     7261 aactcctata attgttacaa gagttcgagt gcgattcttt ttcaatcctt gcagagcaac
     7321 tttgttaaaa atgttcatta acgattcacc ttcctctcat cacgcactac ttttccatct
     7381 gaaatacaga taattctatc tgcctgtaaa gcaatgtttt catcatgtgt gacaataatg
     7441 agagtttggt gatatttact atggctctct ttcagtaact ggataatctc ttgtccattt
     7501 ttactgtcta agctacctgt aggctcatca gcaagcataa ctgctggtgc attcatcaat
     7561 gcacgaccaa tggcaacacg ttgctgttgg ccaccagaaa gttgatttgg taaatgtgtt
     7621 tttcgttctt ttaaaccaag caattctaac aagtcattta accgttcttg attgacctta
     7681 cgcttatcca taagtatagg caatgtgata ttttctacta catttaatgt gggtatcaga
     7741 ttgtgaaact gataaatcaa tccaacttgt cgcctacgaa aaatagcaag tttttcattg
     7801 ctttggccat atacatcttg cccctccaga tatatctttc cacttgttgg tacgtctact
     7861 cctgcaatgg aatgtagtag tgtagattta ccagaaccag aagaaccaat gattgcagta
     7921 aactctcctt tttcaatagt aagtgagaca tggtcaagtg cagtaacctt attttcattc
     7981 ttaccataaa ccttacataa atcttcaatc tttaaaaacg ccattatata agtctccttt
     8041 catattttat aaatatatca taggactttc aacttacatt taagtgacat ttaaatgaga
     8101 gattcgtcac tttggaaaac gaataacaaa tattgcacca ccttgtgggt gattttgtgc
     8161 agtaattgtt cctccttgtc tcataataat cgtcttacaa agtgccaatc ctattccata
     8221 accggctgtg cttgaatttt tcccacgata aaatctgtta aatagacatg gtaaatcttc
     8281 ttttttaaag cctgctccgt tgtcatgaat agtaagttga gtaaacaaaa agttgtcctc
     8341 acaaatgata tcaatttttc cattatcacc cacactctcc atgcagtttt taaatatatt
     8401 ttgaattgct tctgaaagcc aatttaaatc acccaaaatc cttactcctt ttggtatatc
     8461 aatatgtaaa gtgatattgt gcagttccat gggaatcaaa agttgatgaa gtgcagaact
     8521 aatcaaatta ttcacatcaa tttgttcttt ttgaaagact acaatacctg catctaaacg
     8581 tgataatttt aaaagggaag taatcagcca gtccattcgt ataagtaatt cctctgtttc
     8641 tcgtatcaac tcttttctct catcttcatc agaagtattc tctaacagtg acaaaataag
     8701 agttactgat gtaagaggag tgcgaagctg gtgggctatg tcagctagtg agtcagcaag
     8761 gtgttctttt tcttttttca atgcgtaatt ttgctctcta atacgtaacg tcattttagt
     8821 tatttcactt tgcagaatag aaagttcacc ctcatctgat tcagcaatat acagatggtt
     8881 agcattatga agcacaagat taatttgttc tgaaatctgt gcaatacttt tgtatcgatt
     8941 tttagtaaac acaaaaaatg ctattccaaa ggtagtggca gaaacaatag aaagtattcc
     9001 agtcataata ctgattgcaa atcccagggc tacagtgaga atagtaatta ataagaataa
     9061 aatagcaaat tttcgaaact ctctatttcg aaacataact tccccccaat ctataccctg
     9121 tcccacgaac agtcaaaatt atttgtggac ttgaagggtc atcttctatc ttctctcgca
     9181 agcgtttgat gtacacagtc aatgtgttgt cattaacaaa ctcacctgcc gcatcccaca
     9241 attcatcaag taatcttacc ctcgtaataa tacttttagg attgctaata aacaccaata
     9301 acaaacggta ttctaaagct gaaagaaaaa cctcattgcc attctttttc acaacaccgg
     9361 ttgccgtatc tacataaagc ccacaaattt caaaagttga cccaaaacgc ccactttttc
     9421 gtagtgctgt tctaattcgc gcaatcagtt cacgtggacg aaaaggttta gtaatataat
     9481 catctgcacc catattcagt ccagtaacaa cacttgattc atcaccggaa gctgtcaaaa
     9541 agataacagg aatgtcttgc ttttctttga tttctgtgca aatcgtaaac ccatttccat
     9601 caggtaaaga aatatcaata agtgccaaat caaatttatt cttagcaatc tctacaagtg
     9661 cttcacttct tgtggataca tgggttacta taaatccttc tgaattaagc aaaagaataa
     9721 gatttttggc aattgcttta tcatcctcaa ctaaaaatat ccgtttcatt tactttcatc
     9781 ctcctttatt ttattagttt tatatattcc agcagagcaa tagtacacta ctattttact
     9841 tatcattaca caattttcag tagtttcttt tgttcttata taatccatgc ataatacctc
     9901 cattgattta ttatatttct tataacataa atacacaaga taagacaaaa aacataatgt
     9961 aagtgcaagt tttttaactc actaacttat aaacatacac tcctactaac actactttct
    10021 tgatgacaca tcttacaagc ttatattcat acatcaatga aaaattaaat ttatccattt
    10081 attaaaataa aaggcaatag cttctatagc taatgccctt tatcttaaaa tgatttaaat
    10141 actatacaat ttttataaat ggctataaat aaactcgtat agaatataaa atttatctac
    10201 agtcttttgt tatacaaaat ttataacctc ttatagaaca aatttaccac tttgcaaact
    10261 tatcactttt actcagctat attataaact tttaatttat ctttgtttaa atgtttcttc
    10321 ttaacttcta ctaaagcttt tagcgtatcc aaagatgtca ttatttcagt agaaaattca
    10381 atggctgttc ttctcatctt aaatccatct cttgtagagt catttccttt agttggtgta
    10441 tttattacta tatcaacttg tttatttctt atagcatcta atatgtttgg tctaggctct
    10501 tcaattctat ttactatatc agcctctatt ccattttctc ttaaactctt cgctgttcct
    10561 tctgtagcaa caaaagtata tcctaattct ttcatatctt ttgctatctc tataaactca
    10621 tctttatcat gattatttac agtagcaagt actactcctc tttcatcaga catatgtcta
    10681 cctgctgcca agaacccttt atataaggct tcttctaaat tttctcctac acctagaact
    10741 tctcctgtag acttcatttc aggtcctaga cttacttcaa ctttagataa tttagacatt
    10801 gagaatactg gtacttttac tgatactaat tttggctctt tgtaaactcc tgtactatat
    10861 cctaaatcct ttaatttagc tcctaacata cacttagtag ccaagtcaac tattggtact
    10921 ccacttactt tacttatata tggaactgtt ctactagctc tagggttaac ctcaattata
    10981 tataattcat tttgaaattc tataaattgt atattaacca taccaagcac attaaggtct
    11041 aaagctattt ttttagtgta ttctactatt ttttctttta tttcatcaga tatattttga
    11101 cttggataca ttgttgtact atctccagag tgaactccag ctctttctaa atgctccatt
    11161 ataccaggta tcaatatatc ttctttatca catatagcat ctacttctat ttctctacca
    11221 gttagatatt tatctataag tactggattt ttatggtctc tatcaaatgc atcatccaaa
    11281 tactgaatta gtttttcttc gttataagtt atttccatac cttgaccacc aagtacataa
    11341 gatggtctaa ctaatactgg ataacctaac tccttagcaa tttcaacacc ttcattagtt
    11401 gtccatacac cttttccttt tggtctattt atatctagtc tctctagtaa atcatcaaat
    11461 ttttctctat cttctgctgc atctatatct ctaaagtctg ttcccagtat aggtatattt
    11521 ttttcatgta aaaacttagc caattttata gctgtttgtc caccaaactg tagtattaca
    11581 ccttctggtt tttctttttc tattatattt aaaacttctt cttctgttaa tggttcaaaa
    11641 tacaacttat cagatgtatc aaaatccgta cttacagttt cagggttatt gtttacaatt
    11701 atagtttcta ttcccatttt tcttaaagat tttacacagt gaactgaaca atagtcaaat
    11761 tctattcctt gacctattct tattggacca gaacccaata ctacaacttt tctcttatca
    11821 ctaactacta cctcatcata ttgttcataa gttgaatagt aataaggtga tagagcttca
    11881 aattctcctc cacatgtatc aaccatttta taaactggtt gtatattata taaacttctc
    11941 aattcatata atttttctgg gcttatcttc attaagtcag atataccttt atcagaaaat
    12001 ccctttttct ttaattcatg taaatattct ttatctaagt cttctatttt tagaactttt
    12061 aacttttctt cttgttctac tatccattta aatttattta taaaccactt atcaacacca
    12121 gttatttctg atatcatttc tactttatag cctcttctta tcatttcagc taagtcaaat
    12181 agtctctcat catcaggtac tactactcta gcctttaatt cttctaatgt tctaccttct
    12241 gatggagcat gaaccaatga atattttcct gtttcaagtg atcttatacc tttaagaata
    12301 gcagcctcaa agttacttcc tatactcatg atttcaccag ttgccatcat cttagttcct
    12361 aattttctat tggcatgtct aaatttatca aatggccact ttggtatttt tactaccaca
    12421 taatctaatg ttggctcaaa acaagcataa gtttttttag ttactgcatt ttctatctca
    12481 tccaaagtat aacctaaagc tatcttagct gctacttttg ctattgggta tcctgtagct
    12541 tttgatgcta gggctgatga cctactaact cttggattta tttctattat cgcatattct
    12601 aaactatgtg ggtttaaagc tatttgaacg ttacatccac cttgaacctc tattgcattt
    12661 attatgtcta ttgatgcctt tctaagtagt tgatactctt catcacttag agtttgactt
    12721 ggagcaacaa ctatactgtc tcccgtatga actccaactg ggtctacatt ttccatatta
    12781 catacagtta tacagtttcc atttccatcc ctcattactt cgtattctat ttctttccaa
    12841 ccttttatac tcttttcaag aagaacttgt ccaacaggac ttagctgtag tccatgtgaa
    12901 agtatttctc ttaattcttc ctcagtatca gctatacctc caccagttcc acctagcgta
    12961 tatgctggtc ttacaactac tggatatcct atcttattgg caaactcaag acctgcttct
    13021 aaatttgtaa ctatatcact aactattact ggttgattta tttctttcat tacttctcta
    13081 aataaatctc tatcttcgcc cttttttata gattctatag atgttcctat aacttttaca
    13141 ttatatttat ctaatatacc agcatcatgt aattctacag ctaagtttaa tcctgtctga
    13201 ccacccatac ctgcaagtaa actatctggt ttttcttttt ctattatttt ttctataaat
    13261 tctattgtta atggttctat atatatttta tctgcaattt ctttatcagt cattatagta
    13321 gctgggttac tatttactaa tacaacttca attccctctt cttttaatgc ttggcaggcc
    13381 tgtgttcctg aatagtcaaa ctctgcagct tgacctatta ttattggccc tgaccctaat
    13441 actaaagttt tctttatact atctaattta ggcataactc tttctcctta tctaaacaat
    13501 tttcacagtt cttatcttca tcatctatac aatcaacttt aattacttta ttttatctta
    13561 aattcaaatt tactcatcaa tacatctatt taatacatct atgttaatta aaccaaactt
    13621 tttgatttaa gttattcttg ttttttagtt agctattcta atgttattta ttttactaat
    13681 tctaaaaatt tatcaaaaat atagtcatta tcttttggtc ctgggcaagc ttcaggatgg
    13741 tattgaacac tgtatacatc tagtttctca tgtctcattc cttcaacagt attatcattt
    13801 aaatttatat gtgtcacttt catttgttca ggaacttctg aaacataata accatgattt
    13861 tgagaagtta taaatacttt tccttcttct aaatctttaa ctggatgatt tccacctcta
    13921 tgcccaaatt taagtttagc agtttttcct ccaagagcta aagctaaaag ttgatgtcct
    13981 aagcatattc caactatagg ttttttacca ataagagctt ttatattttc tataacatct
    14041 tctaggtctt ctgggtctcc aggtccattt gacaagaata ttaagtcagg gtttatactc
    14101 aatacatctt ctggagatgt tgtagctgga aatattgtta tatcacatcc acgagctata
    14161 aatgatctta atatatttct ttttactcca aaatccataa cagccacttt tggaccattt
    14221 ccctttatat gctctacttc ttttcttgta acagttctaa ctgcttctgt atttgaaaac
    14281 ttatccaatt tatgtttaac atcttctaat ttactgtctt ctaatgtaat tatacctttc
    14341 atggttccat tatttctaag tatcttagtc aaagctcttg tatctattcc ttcaagtccg
    14401 ataactttat tttgctttaa atattggtct atatcaattt cacatctaaa attacttgga
    14461 gaatcacttt tctcccttac tatgaatccc tttacttgaa ctttttcaga ctctgctgat
    14521 gataaattta taccataatt tcctacaaga ggataagtca tagtaactat ttgaccatag
    14581 taagaagggt ctgttaaaac ttcaccatat cctatcattg aagtattaaa tactacttct
    14641 cctacacttt cttctaaata tccaaatgcc tttcctataa aaactgttcc atcttctaat
    14701 attaaccttg ctttcatact acagtacctc ctataattct ctttagacaa actaaagaac
    14761 attaactaaa tataagcttt taaatatcta tatctatttt tcacttttac attctagttt
    14821 tatacttatt tacatttatt ttttatcttt ttctattata ctacaaattt cttaaaaaaa
    14881 agaagttatc tcaaaagtaa aaactcctag aaaaaataac ctcttttttt tatttaataa
    14941 ttaaattctt aaactataca atcaacttaa tttctattca gctatattgt aaacttttaa
    15001 ctcatcttta tttaaatgtt tcttcttaac ttctactaaa gcttttagtg tatccaaaga
    15061 tgtcattatt tcagtagaaa attcaatagc tgttcttctt atcttaaatc catctcttgt
    15121 agagtcattt cctttagttg gtgtatttat tactatatca acttgtttat ttcttatagc
    15181 atctaatatg tttggtctag attcttctac tctatttact atatcagcct ctattccatt
    15241 ttctcttaag ctattagctg ttccttctgt agcaacaaaa gtatatccta attctttcat
    15301 atcttttgct atctctataa actcatcttt atcatgatta tttacagtag caagtactac
    15361 tcctctttca tcagacatat gtctacctgc tgccaagaac cctttatata aggcttcttc
    15421 taaattttct cctacaccta ggacttctcc tgtagacttc atttcaggtc ctagacttac
    15481 ttcaacttta gataatttag acattgagaa tactggtact tttactgata ctaattttgg
    15541 ctctttgtaa actcctgtac catatcctaa atcctttaat ttagctccta acatacactt
    15601 agtagccaag tcaactattg gtactccact tactttactt atatacggaa ctgttctact
    15661 agctctaggg ttaacctcaa ttatatataa ttcattttga aattctataa actgtatatt
    15721 aaccatacca agcacattaa ggtctaaagc tattttttta gtgtattcta ctatcttttc
    15781 ttttatttca tcagatatat tttgacttgg atacattgtt gtactatctc cagagtgaac
    15841 tccagctctt tctaaatgtt ccattatacc aggtatcaat atatcttcct tatcacatat
    15901 agcatctact tctatttctc taccagttag atatttatct ataagtactg gatttttatg
    15961 gtctctatca aatgcatctt gtaggtattg agataatttt tcttcgttat aagttatttc
    16021 cataccttga ccaccaagta cataagatgg tctaactaat actggataac ctaactcatt
    16081 agcaacttca ataccttcgt taagtgacca tacacccttt ccttttggtc tatttatatc
    16141 tagtctctct agtaaatcat caaatttttc tctatcttct gctgcatcta tatcatcaaa
    16201 atcagttccc agtataggta tatttttttc atgtaaaaat ttagccaatt ttatagctgt
    16261 ttgtccacca aactgtagta ttacaccttc tggtttttct ttttctatta tacttaaaac
    16321 ttcttcttct gttaatggtt caaaatacag cttatctgat gtatcaaagt ctgtacttac
    16381 agtttcaggg ttattgttta tgattatagt ttctatgtcc atttttctta aagattttac
    16441 acagtgaact gaacaatagt caaattctat accttgacct attcttattg gaccagaacc
    16501 taatactatc acctttttct tatcactaac aactacctca tcatattgct cataagttga
    16561 ataatagtaa ggagatatag catctatctc acctgcacaa gtatcaacca ttttatatgc
    16621 tggtttgata ttgtatagac ttctcaattc atataatttt tctggactta tcttcattag
    16681 gtcagatata cctttatcag agaatccctt tttctttaat tcaagaagat agtctttagt
    16741 taaatcttca attttcataa cttttaattt ttcttcttgc tctactatcc atttaaattt
    16801 atttataaac catttatcta tacctgtaat ttgttctatc atttctactt tatagcctct
    16861 tcttatcatt tcagctaaat caaatagtct ctcatcatca ggtactacaa ctcttttctt
    16921 taattcctca atgcttctct cctcagatgg tgtgtgaact aatgaatatt ttcctgtttc
    16981 aagtgatctt atccctttaa gaatagcagc ctcaaaatta cttcctatac tcataatttc
    17041 accagttgcc atcatcttag ttcctaattt tctatttgct tttttaaatt tatcaaaagg
    17101 ccattttggt atttttgcca ccacataatc tatagttggt tcaaaacaag cataagtttt
    17161 tttagttact gcattttcta tctcatctaa agtataacct aaagctattt ttgatgaaac
    17221 tttagctatt ggatatcctg tagcttttga tgctaaagct gatgacctac taactcttgg
    17281 atttatctct ataactgcat attcaaaact atgtggatta agtgcaaatt gaacgttaca
    17341 accaccttga acttctactg catttaatat atctaaagat gcttttttaa gcattgcaca
    17401 ttctttgtta cttaaagttt gagttggagc aacaactata ctgtctcctg tatgaactcc
    17461 gactgggtct atattttcca tgttacatac aactatacaa tttccctttg aatctcttat
    17521 tacctcatat tctatttctt tccaaccttt tatactcttt tcaattaata cttgtccaac
    17581 aggacttaat tgtagaccat gtgaaagtat ttctcttaat tcttcctcat tatcagctat
    17641 acccccacca gttccaccta gtgtgtatgc tggtcttaca actactggat aaccaattgt
    17701 aagtgcataa tcaagacctg cttgtaaatc tgtaactata tcactaacta ttacaggctg
    17761 atttatttct ttcattactt ctctaaatat atctctatct tcaccttttt tgatagattc
    17821 tactgaagta cctattattt ttactccata tttatctagt atgccttttt cataaagttc
    17881 aactgctagg tttagccctg tttgtccacc catacctgca agtaaactgt ctggtctttc
    17941 tttttctatt attttttcta taaattctat tgttaatggt tctatatata ttttatctgc
    18001 cacttcttta tcagtcatta tagtggctgg attactattt attaatacaa cttcaatccc
    18061 ttcctctttc aaagactggc aagcttgtgt tcctgaatag tcaaactctg cagcttgacc
    18121 tattattatt ggtcctgacc ctaatactaa agttttcttt atactatcta atttaggcat
    18181 aaatttttct ccttatctaa atggttttta ctatctgtaa ataatactaa atctatatta
    18241 atgctaagaa tttattaaat atacaatcta aatcttcttt tgttgtgtat tctggaatgt
    18301 actgaacact atatattggt aattccttat gtctcattcc ttctatagta ttgtcattta
    18361 aatttatgtg agttacttct gtattttctg gcatttctga aacattatat ccatgatttt
    18421 gagaagttat aaataccttt ccagtttcta aacatttaac tgggtgatta cctcctctat
    18481 gtccaaatac aagctttgaa gtctttcctc ctaatgccaa tgctaaaatt tgatgtccta
    18541 aacctattcc tgtcaatggc tttttgccta ttaatttttt tatattttca actatatcat
    18601 ttatatcctc tggatttcct gggccattag ataataagat taagtcagga tttatattta
    18661 aaatttcttc tactgtagtc attgctggta ctatagttat atcacagtta caagccttaa
    18721 gagactctag tacactattt ttaacaccaa agtctataac tacaacttta gctccagtac
    18781 cttttatatt ttcaatttct tttcttgtaa cattttttac tgaatctttg ttacaatgct
    18841 cttctaattt agactgaaca tcttgtaatg atacattttc taaagttata attcctctct
    18901 tagttccatt gtttctaata attttagtta atgctcttgt atctattcct tcaagtccta
    18961 ttaccttatt ttgctttaga tatgtgtcta tatccatttc acatctaaaa ttatttgggt
    19021 tatcactttt ttctcttaca ataagtcctt ttacttgcac cttatttgat tcaacatctt
    19081 ctaaatttat tccatagttt cctataagtg gataagtcat agtaactatt tgtccataat
    19141 aagttgcatc tgtcaatagt tctccatacc cagccattga agtattaaat acaacttctc
    19201 caatagcttc ctttagatat ccaaatgctt ttccttcaaa tactgttcca tcttctaaaa
    19261 ttaatttccc cttcatgtaa gctacctccc aaatatttta tctaagcatt ttctatttct
    19321 cttacaatat taatagctga ttctcttgga ttttctgctt ttgttatagg tcttccaaca
    19381 actaaataat gagcaccttt ttttattgca tctgatggag ttacaactct tttttggtct
    19441 ccaacctctg ctgatttagg tcttattcct gggcaaactg ttataaaatc agttccacat
    19501 gcttctacta ttttatctac ttcttgcgct gaacaaacaa ccccatctat tcctgattct
    19561 tttgccagtt tagctctctt tatagctaac tcttctgttg tcatattaca tcctatgtcc
    19621 tgtaaatctt ggttgcttaa tgaagttaaa actgttactc caactactaa tggcttttct
    19681 atatttaatc tttctgcttc ttccctagca attagtgcac attgtctcat tccttccata
    19741 tctgatacat gtattgtcat taaccaaaca ttatctctca ctgcagcttt cactgcactt
    19801 ttcatagtat ttggtatatc atggaattta aggtctaaaa atattttttt acctttttct
    19861 ttaagataat ctattgtttt accttttgtg gcaacgtatt gctctagccc taccttaaat
    19921 atgtctacac tatcttctaa tctgtctatt agttctttag ctttatcaaa ttcgtcagta
    19981 tcaattgcaa ctatcaattt ttctttacca tttatcattt ttacatctcc tttttaataa
    20041 agcctaaata atcataaaaa aatcctcaca ccaaaagagg cataaggatt ttatctatac
    20101 ataactctat tcattagcta tgagatatct ttcccagtct ctctggacta gtttaaaaga
    20161 ctttattata tttaaatttt tattttatac tcatcaaaaa cctctagaag atttactaga
    20221 ggttatatac taacattatc tataaccctt tagtctctct ggactacttt aaggaattta
    20281 gctgtaatat ttttctttat ttatataact ttatgtatct ataggataat ttaaagtttt
    20341 atctttttta taattaaacc actaattgct ttttatgtca ataagtcttt gaattattct
    20401 tcatctttat caaacaattt tttctcaaaa tatgattctg cattatatat tgcagcaact
    20461 ggattatgcc cagttactct atctttgaca actaaagttg ttataggagc ttccatatat
    20521 ttcatggcta aagtatcgtg accaacacat aatccaagaa ttacattaag gtcaacctta
    20581 gcttcattta aaaataaagc ttgtcctatt ggattgcaca ttacctcatc tgaacacttt
    20641 gatattgttt gagatgcttc tagtccaatt gtactcttag gcattgcccc atttttacat
    20701 ataacagaaa atacttcaaa cccatgatgt tcaaatattt tttgcactgt ttgcatttct
    20761 ctttttagtc caatacaaaa tgctatgcca atctttttat aaccacactt tttagcaaac
    20821 tctataatct cttttagtct agtatattga caatacccct ctgcttcaac taatccagca
    20881 ttataagcta tttttctatt ctcttcttct tcataaagtt gctttgcttt ttgttgtagt
    20941 tcaatttctc tagttggaca tatcttcatt aatttatcca tatttccatt agaacagcct
    21001 ctaagattac attttccaca cttatacata tccaatatcc ccttccaaaa ttttattcat
    21061 cttgcgattc ttctttttct agagttacat tttcttcttg ggtttcttct tctggtttca
    21121 catgctcgac tacagttata aaatatgatt ttcctatttt attccttata ggaagttttt
    21181 catatgaaac atctatttta ttttttatac aagctatatc aaaatgtctt aaggctacac
    21241 ctaagctaac cttattcata tcttcacagt gtttcttagg tctcttttca aataaatgaa
    21301 gacctcgttc atccttatgg aaataccacg gttgagaatt ttttacagat ggagctcttc
    21361 ttgtcaattc taagatatct ttccactttc tattcctatt tatcttattt atcatatctt
    21421 ttactggctt tctatcaggt ctagcatatc tcgtcctaaa caactcttca ttttttgctg
    21481 gatatccaaa tgctattatt atataaggat tttctatttt gttttcatca tcttcttctg
    21541 tttttattcc agctagataa tctaaatcat cttcctcatc ttcttcaaaa ttcttattcc
    21601 caatatctac aaattccaag atatcttctc tctttaagtt tccttccaac cagcaagtag
    21661 ctattcccaa tgttgtaagg cgcaaaacta caccttctgt agcaaatcct atattctgta
    21721 aatagtcctc acctttgtct gatgtaacta ctatataatg aggtgccttt actttacatt
    21781 ccttacccat caaaaaatgt attatatgac ctctatcaac aacatgtgct ttaatattta
    21841 aatcttcatt taagtattct aaatttttac aacttctttt tatttcttcc atcaatggct
    21901 tttttatctt tttactggaa aaatttctta cagattttct ataaaaaatg gcttcataaa
    21961 gctccattta tgccctccta actgaaatct tctgacgtat aaactctata aatttatata
    22021 aatactttat attttttata gttttatttc ctttttcctc ctgccctatt ttgaaattca
    22081 taagaatatc tactctagtt taatatagca ttcaaaaggc ttaaattcta ccttatcaat
    22141 gtctatacat tagcataatt tttttatttg attttctatg ctaatttaaa taacaacact
    22201 agctattaac tttttaatta agctttgcca gaaaaatttt ttttcattta taaatatcta
    22261 tctcattatt atattataag tttttttcgg tattcttgta tagcttttca ctaaaaaagt
    22321 atttttattt ctactttaat attattatca acaaaaaaca tactgcttaa ataagtttgt
    22381 tatcctcata atctgtttta attaaaatat tttttttatt tctcaatcta taaagtataa
    22441 atattttgaa tatgacccaa aatctaagta aagttataat attttataag ttgttcaata
    22501 ttaaaaacat aagtaatgtt ataatacttt ataagttatt caatattaaa aatataggag
    22561 gataaatatg aatttaaata gaaatttaga aaaatacgct gaattagcaa taaaagttgg
    22621 agttaacata cagccaggtc aaattctact tataaagtca ccaatagaat gtgctgattt
    22681 tgcaagaaat gcacttaaag aagcatataa atgtggtgct aaaaatgttt atatagagtg
    22741 gtctgatgaa gaaagtactc ttattaaata cttatatgct ccagatgaag catttcatga
    22801 gttccccaaa tggacagcag aacaatatgt agatatagca aaagaaggtg gggcattttt
    22861 atcagtctat gcccaaaatc cagatttatt aaaagatgtt gaccctgaaa gagttgcaaa
    22921 ttttcagaag gcatctggaa aagctttaaa agaatggcgt tcttacactc taactgataa
    22981 atgtaaatgg agtatagtct ctgtgccaac aaaagattgg gcaaaaaaag tgtttccaaa
    23041 tttatcagaa gataaagcaa tagaaaaact gtgggatgct atatttaaat gttcaagagt
    23101 agatggtcag gaccctataa aagcatggga agaacataat gaaaatctta aatctaaaat
    23161 ggactttcta aataaaaata actttaaaac tttaaaatat aaatcctcaa aaactgattt
    23221 gactctagac ttgccaaagg gtcatgtttg gctaagtgga gcatccaaag accctaatgg
    23281 aataagtttt aatccgaata ttccaactga agagattttt ggaatgccac ataagtttaa
    23341 agtaaatgga actgtttata gtacaaaacc tcttgtttat ggtggaaaca taattgacaa
    23401 tttcttctta acatttaaag atggtaaaat aatagatttc tctgctgaaa agggactcga
    23461 tacactagaa aaacttattg aaacggatga aggctctcat tatttaggag aagtcgctct
    23521 tgttccatac aactcaccaa tatccaatac ggatataatt ttttataata ctctatatga
    23581 tgaaaatgct tcttgccact ttgcaattgg ttctgcttat aagacttgtc tggaaggtgg
    23641 aaataattta aaagatgaag aattagatga acatggtgta aatgacagtt taacacatgt
    23701 agattttatg attggttctg ctgatatgga tattattggt gagacttatg atggaaagca
    23761 aatacagatt tttaaaaatg gtaattgggc tttttaacat atgattttaa ctaaaaaggg
    23821 ctgatggaag ttataccaga cttcccagcc ctttttaatg ttgatgataa atatttttta
    23881 tattaattaa tctcattatt atagattaat tcctattgtt ttaaacataa gtatctaaaa
    23941 cttatattta tattatatat aaaatatcct atttagtaaa acctttattt taatttatat
    24001 gctaaaaatt taactttgct atttcaacag cagactttgc atcctttgaa taataatcag
    24061 cacccatttc ttcagcgtat tcttctgtca aaactgcacc tcctacaaat acctttgcat
    24121 ttatctcatt atctctcagt gcctgaattg tatctttcat actttgaaca gtcgtagtca
    24181 taagtgcact taatcctact aattttatat ttctcttttt agcaacttcc actacttctt
    24241 caatagggac atctttacct aaatctataa cttcatatcc ataattttct agcattattt
    24301 ttactatatt tttcccaata tcatggatat caccttttac tgtagcaact attatttttc
    24361 ctttggatac attattgcta cttttactta ataaagtttc ctttattgta tttaatgaaa
    24421 cttttaccgt ttctgctgat tgaatcaatt gtggtaaaaa tatatctcca ctatcatatc
    24481 tttttcctac tttatcaaga gcaggaatta aaatctcatc taatatataa ttttcatcct
    24541 tctcttttaa aagattcagt gttacatttt ttgcttcatc ttttaatcct ctttcaacta
    24601 aaatatccaa gggcaaatct ttatcaatct ttgtagatga atccgatttt tttgaattat
    24661 ttatattgct atattgattt atatagttta tacatccttt atctgtgtta tttaatattt
    24721 taaaagaatt gatagcttcc atcatactat attcatttgg atttataatt gctaaatcaa
    24781 gccctgcccc taaagctaat gttagaaaag aagcattaag tgctttccta tttggaagac
    24841 caaatgaaat atttgaaaca cctaatatag tttttactcc tagtgtcttt accattgtta
    24901 tagcttttat agtttctatt gcttcttctt gttgtgctga aacagttaat gataaacagt
    24961 ctataaatat atcttttggt tttatcccat aagaaactgc tctattaact atcctttttg
    25021 ctatttcaaa tctttcttca gcctttttag gtatcccctt ttcatcaagt gtaagtccaa
    25081 ccacacatga accatacttt ttaactattg gtaatatact ttctaaagac tcctctttgc
    25141 catttacaga gtttactatt gttctaccat tataatatct aagtccttgc tctaaggcct
    25201 ctacattgga agaatcaact tgtaaaggtg tatctactac tgcttgaatc tctcttatta
    25261 attttggcat tagttttttt tcatcaatct ctggaagacc aacatttact ccaagtattt
    25321 gtgctccagc atttacttgt tctaaaccta aatttatagc ataatcaaca ttttcatttt
    25381 ttaatgcttc ttgtagagat tttctacctg tgggattcaa tctttctcca acaactgtag
    25441 gcgattgtac ttccacaaat tttgatggtg aacagactac acatttatca attttctcga
    25501 tacatccctt tgtaacagaa tttatattct cagatatttc ttttataaaa cttggattcg
    25561 taccacaaca tccacctatt atagtagcac caacttcaac gaacttttta accccttcaa
    25621 aaaattcttt tgcatcaaca tcatatatag cctctccatc aactatattt ggaagacctg
    25681 catttgcttg aaccataaca gggactgtag ctcttgatgc tattttttca actattggta
    25741 aaagttgttt aggtcctaat gaacaattaa ctcctattgc atctacacct agaccttcta
    25801 tagtagccac catacattct ggcatacatc cagtaaaact tcttccacct tcatcaaatg
    25861 tcatagtaca gaaaataggt aaatcagagt tttcttttgc tgctaataca gctactttag
    25921 cttcatataa atccatcata gtctctatta ctattacatc aactccagat ttaacgcctt
    25981 gtagtacttg tcttttaaat atttcatatg ctttatcaaa acttaaagta cccataggct
    26041 ctagcatttc tcctatagga ccaatatcta aagctacata tcttggttta gtattatcta
    26101 cattttctat agcttttcta gccaccaaaa cagcattgtc aatcacttct tctacagtat
    26161 acttactatc taattttagt tcattacatc caaaagtatt tgtaagaatt acattcgaac
    26221 ctgcctctaa gtatgctgta tgtatttcta taagtttatc tggattttga agaccaaata
    26281 cttctggatt ttcacctaac tttaaccctt tttgttgaag cattgttccc attgcaccat
    26341 caaatattaa tatattattt ttcagatatt gtcttatttc cacaagagtt cgctcctttt
    26401 ctatatgtac agtttctata gtttttacaa ttttcacacg acttttcttt cttagattta
    26461 tttattcttg tattagaaat acctataata gccactactg actttctcgg tatcataatt
    26521 ccattagaag taattgttag acctatttgt ttttgagcat tcaaaacgtt taatatatct
    26581 atattttttt ctattggtaa atctccatat cctgggctat atcgcatagt tataaattta
    26641 tcttgcttta aaaaaacctt ttctaaatta ttttgaacaa tgtcacacac ttcttctatt
    26701 gctgttgttc cacatgaatc aattattagt ccttttgtta aatttgaata agagtgtcgt
    26761 cttatttctt tttctatatc tatccctata gtagctgcca taattacaca ctcattacat
    26821 tcttttaaaa gttcatatac atctttactc tctaatgtta aatttgttcc ctctaattct
    26881 acaacattat ctttatttat tttaatagga taaatcctag ataaatatct aggatttatt
    26941 ttatttttag ttatagctac acattcatca atagtatatt taaggtcttt atctatctct
    27001 tgacctctgt attgtaaata tcttaaaact tcactttgat ttatgcttat atcttctctt
    27061 ccaattaaat tattctccat atatatttaa agaaaatcct ttatctaaaa catcttttgc
    27121 atattccttt gccccaaata aagataagtc tctgtagttt ccacattcta tagctgtagc
    27181 tgctggcatt ttatctgatt caagaacttt ttttaatgct tcttctaaac caatcttaac
    27241 tgttttggca tctacatctc cccatagtat taaataaaat cctgttctac atcccattgg
    27301 agataagtct attacaccat ctaatgtatc tcttaaataa gtagctaaaa gatgctctaa
    27361 accgtgcata gcagcagttc caaaactttc tacatttggc tgtaaaaatc tcaagtcaaa
    27421 tttagttact ttatctccct ttactccatc aagtacacta cactttctta caaaaggagc
    27481 ttttactttt gtatggtcta gcttaaaact ttctactttt tccattattt aatcctccat
    27541 tatactttaa aatttgttac attaattcta aaaaaaactc ttcctatcta ttattgataa
    27601 gaagagtata ttatttcaag tcatctcatc gttgttagca ggaccacctg ataaaaggtt
    27661 ggtataggat cactgagcta actctctccc ctaactcttg ataagtatct tattaaactg
    27721 acttaaatta tatattactt ttttattttt gtaaacactt tattgttata atatttatat
    27781 cacataatcg tacttttatt ttataactaa gctgattgta ttattggcag ttgtacttca
    27841 aacacagttt gatatttatc gcttgaaaca gtaatttctc catcatgtaa atttacaata
    27901 ttttttgtaa tcgcaagacc caatccagaa cctccaacat ttctatttct cgatttttca
    27961 actctataaa atctatcaaa tatatttggt aaatctaaca ctggaattgg ttccccataa
    28021 tttatcactt ttacaaccgc catatcttcc tttttctctg tgactatatc tacataatgc
    28081 ccatcttttc catatttcac agcattagtt ataagatttt caaatgctct taccaattta
    28141 tttggatcag cttttacaat taatttttct tcattaaaat taattctact aaccataaat
    28201 tcttgtttaa aataaatttc aaattgagat actacttgac ttaataactc tactaaattt
    28261 atttctattt tgtttaattt tattgtatta ttttgcattt tagtcaattc aaacaaatca
    28321 tttataagaa catttaaatt tttcgatttt tcatatgcaa tatcaacata atacatcatc
    28381 tgaacttcat ctttatactt accttcttct atcactccta aataacccat tatggatgtc
    28441 aaaggtgttc ttaagtcatg tgacacattt gttattaaat ctgtttttgt ctgctgagct
    28501 tgtctctctt caatagttat atttctaagt tgatggacta tattattgat tttatgtgct
    28561 agttcatcaa tatcatcctt ttctttatcc tttaatatat ctaagtcata ctcaatatct
    28621 atactttttt ctaagttacc ttcatccatt aaatttacag cacttttaat gatttctaat
    28681 ttttgatttt ttctattagt ctttcttata aaatctacaa tttgcttatt tctcttgtac
    28741 gatatcatgg aataaattgc taatgatgtg attgtaaata aaaatggata aaataaatca
    28801 taccctataa gacttctgaa atctcttgct tgatatacaa gttgattaac tacagacata
    28861 tcactattga catataatga ttttattata tacatacttg ttaaaataag cattgaagtt
    28921 aaagcacagc ttattataag atctaatatg ggtcgaaaca tatgatttaa taaactttta
    28981 tttttcaatt ttatatccta ctccccatac agtatgaatt attttttctc catctgtata
    29041 tttttcaatt ttatccctta ttctgctcat atgaaccatt acagtattat tagattggta
    29101 atatttctct ttccatacct tttcaaatat ttcttctgta ctatatactc tgcctttatt
    29161 atttgctaat aaatataata tatcaaattc tcttgatgtc aaaattattt catttccagc
    29221 cactgttaca ctatgttttt ccttatctat aattactact ccaaccttta tgacatcttt
    29281 atcttcatta ttagcaccac taaaataata tgctcttcta atcagcgact tcactctcac
    29341 agtaagttca agtgggttaa atggttttgt caaatagtca tctgcaccag tcattatccc
    29401 ttgtatttta tccatgtctt ctgattttgc acttaacata agtattggta tattcaaact
    29461 ttctctaact tttctacaaa cttctatccc atccatttta ggcatcatta tatccaatat
    29521 cattaaatga atattttcct tttttaaaat atctagagcc tcttttccat cttcagcttt
    29581 aattacatta tatccactat ttaaaagata tatctctata aggtctctta tttccttttc
    29641 atcatccaca acaagaactg tattattcat ttttacacat cctaacttaa tctttgtata
    29701 tagctttatt catataaaat tatactatat tttagttagt gtaatctata aatttattaa
    29761 tttctaaatt tccaatataa tacaaattat ttacacattt atattattta ttttgtaaat
    29821 attcttctaa agttaatcct tttgaataaa tttcctcggc agcatcctta ccaacatatc
    29881 taatatgcca tggttcatat gctgtacctg ttatatcttc tttaccttca ggatatctta
    29941 ttataaatcc aaacttgtga gcattttctg ccaaccattt agcttctttt gtacttccaa
    30001 caaaccatct ttctggattt gttaaatcaa tacaaagtcc agtttggtgc tcactgtaac
    30061 caggctttgc tacatatgca tctgcctttt ctcgtccctg agattttacc cttcttgtat
    30121 aagtatccag ttgagtatca taagatctgt atgctgaaga ccctaaaaac tgtattcctt
    30181 ctgaattagc ttgcttaact aaatcttcaa ctgcctttgc aggttctcct gccatttgtt
    30241 tttcttcctc tgttgcttct tcaacaaaag gtatgtttac cttagtaata ttttcaggag
    30301 tatagttttt gctaattcca tttgtcttgt ttaccaataa tatatttgat atactatttg
    30361 agtgattttt attttgagaa tttgcctcaa catcttgttc tgaatcatgt tgagcagcta
    30421 ctattggatc tttatctttc ttaagtaatc tatct
//
LOCUS       DAELLV010000022        30343 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.022, whole genome shotgun sequence.
ACCESSION   DAELLV010000022 DAELLV010000000
VERSION     DAELLV010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30343
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.022"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            14..2662
                     /gene="polA"
                     /locus_tag="KPD58_000853"
     CDS             14..2662
                     /gene="polA"
                     /locus_tag="KPD58_000853"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBG0264637.1"
                     /translation="MEKKLIIIDGNSIINRAFYALPEMNNKEGLKTNAIYGFTTMLFK
                     MIDIYKPTHISVAFDRKAPTFRHLEYKDYKAGRKGMPDELAEQLQPLKDLLDKFRINR
                     LEIDGYEADDIIGTVSKKAEENGYKVYIVTGDKDAIQLASDNTTTLITKKGVGEVEEY
                     NFNSVIEKYEMTPTQFIDLKGLMGDKSDNIPGVPGIGEKTGIKLIKEFSSIENLIENT
                     DKLKGSVKKKIEENKEIAIQSKRLATIIRDVPIEVDLDSMIFGDYDKDELLDKFRYFG
                     FTSLLSRLVDLVDSDDTEHVEEKIEILKLKDIEKFIDEVNKKGQVILKTVRGQGNILE
                     KNIIYIFISVDGEKIYYVDSNEVSKLDSIFSNNEIKKYGYNLKDDYISLKPYNINLEN
                     MYFDITIAEYLIDSTSSTSYDCSAIAMKYLSKKVKSKEELLGKGVKSKNYEDLEFEDL
                     AEYMGQIICTVKETVPMMERSLMDMSMDGLFYHVEMPLVEVLGHMEYEGIKVDKSILD
                     ELSVEFKEIIATLEKEIYELSGEPFNINSPKQLGVILFEKLELPIIKKTKTGYSTNAD
                     VLEKLRDKHPIIDKITEYRQIVKLNSTYVEGLLNIINPESNRIHSSFNQTITTTGRIS
                     STEPNLQNIPIKMEMGRKIRKVFIADDNCKLVDADYSQVELRVLAHMSQDENMIEAFK
                     HHEDIHTKTASQVFNVPMEDVTSELRGAAKAVNFGIIYGKSDFGLADDLNIPVPKAKE
                     YIESYFAKYHKIKEFMDTTVEKASEDGYAVTILNRRRYIPEIKSSNFMERNRGKRFAM
                     NAPIQGSAADIIKIAMINVHKKLEENNLKSKLILQVHDELIVEAIDDEIDIVKKIVKE
                     EMENAVNMDVHLDVDLNVGSSWYETK"
     gene            2671..3273
                     /locus_tag="KPD58_000854"
     CDS             2671..3273
                     /locus_tag="KPD58_000854"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBG0264638.1"
                     /translation="MLILGLTGGIGCGKSSLSNIFRNLNIPIVDADIISRKIFEDKLL
                     LEKVFVHFGQSIKNDDGTLNRKALGKIVFSDEEKLKELNNLTHPRIREKIISEIEKLR
                     KKGENIVVLDAAILVESGFLDMVDKLLVVTCKQEVQISRIQKRDNCSEQEALSRINSQ
                     MSQEEKSKYGDYIIDNSGTITELESKAHKFIEYMKENWRE"
     gene            3266..3820
                     /locus_tag="KPD58_000855"
     CDS             3266..3820
                     /locus_tag="KPD58_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBG0264639.1"
                     /translation="MNSKKVLILSIFIILFGALLMESKVIHKFLYPKKYSEYVEKYSK
                     EFNLDENIVYSVIKAESKFNSSAVSKKEAKGLMQILDITRDWGAEELNLKNVDIFDPE
                     TNIRLGCWYLSKLYKEFGKLDLVIAAYNGGSGNVKKWLENNEYSKDGENLHDIPFKQT
                     SKYVEKVKNNYEHYNKIYGKKGKN"
     gene            3820..5535
                     /locus_tag="KPD58_000856"
     CDS             3820..5535
                     /locus_tag="KPD58_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0264640.1"
                     /translation="MKRIKVLTVVLAITLMVAGCSNTKTKQSSSDSSLQSQSSIDAKY
                     INLTMVTPKTINPITNTDKSVGYIMNLVYDSLFTIDENYNVIPQLVKEYNIAQDGMSI
                     DIKLKDAKWHDGKNVTSNDVNYTIGLIQKSADSPYNEFTKNIASVNIKSDKDFTIKFK
                     ARYAFSIDSLIFPIVSQNHLDSKDVNDKNNNMIGNGKYKIESYTEREGMVLSVNKDYY
                     EEVPKTMKNIKVGMVPNEDARTSMVMALDSDITNVTLNDLSKFQEKEFNITKYQGRDY
                     ECVLFNYNNPFFKDVNFRKAIAHSIDKDRIISEGYMDDATPVNFPLNSKSKYYNSEMK
                     DLEFNKDKAIECLAKVEYANVNSVNQNDNKVKNQKKSAKKNSKKLTPEEEAKAKKAEE
                     DRIKKEAEEKKNREKEEVKKSLSEMNLKIIVNRDNSERIKTANIINENLKAIGINSTV
                     NQLSDKDMENALNSKNYDLALVGWKLSIIPDASSIIASSGYTDDKLNGYMSALTSSTS
                     EIETKKAYKDVQTYIKDNATFISLAVRNNYIVSNSRLKGKITPNDFDVYEGISNLDIK
                     SNESN"
     gene            5608..5820
                     /locus_tag="KPD58_000857"
     CDS             5608..5820
                     /locus_tag="KPD58_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HBG0264641.1"
                     /translation="MKKKLLVEGMSCGHCVNHLKTALTEDIDGIEVLDVDLENKCASV
                     DMKDDVSIEKLKEVIAELGFELKGIE"
     gene            6021..6620
                     /locus_tag="KPD58_000858"
     CDS             6021..6620
                     /locus_tag="KPD58_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264642.1"
                     /translation="MESRGYKEDLKKMILDMAHKELESYVSQKGAKKYFEGNLDKIIN
                     NNIKKFNQSIGLNRVYLKMLKKCAYQESVTSISRTIKKEALFKSKKELFAFSEYLKLD
                     INKRLSYNQILRKISRYIYYNRSSYAQKYVVFKRGDEEYLLEPEKIKDELISSYRSKT
                     REDMRSIAKLLDIEVEDDYNAEDIRKKVINYIIKEKLKS"
     gene            6848..7219
                     /locus_tag="KPD58_000859"
     CDS             6848..7219
                     /locus_tag="KPD58_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG0264643.1"
                     /translation="MKFNFCHNNFNVTNLEKSLDFYKKALGLKEVKRKEAEDGSFILV
                     YLGDGITSHTLELTWLRDWDRPYNLGDNEFHLALEVEEFDEAKKLHKDLDCICFENES
                     MGIYFIADPDNYWIEILPKNH"
     gene            7351..8625
                     /locus_tag="KPD58_000860"
     CDS             7351..8625
                     /locus_tag="KPD58_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBG0264644.1"
                     /translation="MKKAIAALGIGAVAVSVSSINASALEKGTVTASALNIRSGPSSD
                     CDKVAKLYKGKTVEILEKSNGWYKVRVSSSVVGWGSAKYISTSGSSEGTSSQNNPTSS
                     GTTISGNGKVNVSSRLNVRSGAGTNYSLVGKANNGDIVKLLEQSNGWYKIKLSNGVTG
                     WASSQYISKTSEDVGTNNSSNSNSTNNSDKKPSSEESIEGKNGKVTSAVSLNVRSGPG
                     TSYSIIGKLNGGDVVELKAKSNGWYKVKLSSGTIGWVSASYISETNEGTKEKPNSSSN
                     QNSQPNSNSKPSFTGNSDKSTAKGSTIVDFAYTLIGIPYQWGASGPDKFDCSGFTQYV
                     FKHSVGVSIPRVSREQANFGSAISMGNYAPGDLVYFDTDGDGTTNHVGIYVGNSKFIH
                     CSGTQTNPNKVKVDNLTSSYWSKVLLGARRFV"
     gene            8736..9680
                     /locus_tag="KPD58_000861"
     CDS             8736..9680
                     /locus_tag="KPD58_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264645.1"
                     /translation="MNRRKTIYRGFNNNRHKIDLRKYVITIACLCLIGYYSYTKIKDS
                     KILEYVSAKIPFLNNSSDITYKDISDELNSIKKGKKSKSQTNSDDKQETNPEKAVNNT
                     KEPEEVKLATIEGWDMYTIQVAAIDNNDDLKKIQTSLVNNDIPFSVMEKDGVKKIQTY
                     SSFDENDVRKQISSVRKVFPDAFLSHLDAPMLSLEYTSNYAYIESISKELNKLITNFK
                     EESSFWSNAENNVDMEKYNTILTNRKAISQNISKEAEKIDYSEMRLFKDNLIEYVKNV
                     NEKIDTASKAANEEKYSVSKSLLLSSMQEYSMFINSIK"
     gene            9896..11233
                     /gene="rsxC"
                     /locus_tag="KPD58_000862"
     CDS             9896..11233
                     /gene="rsxC"
                     /locus_tag="KPD58_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HBG0264646.1"
                     /translation="MKLLTFKGGIHPPYRKEYSNTKALEKAQAPKIVYIPLQQHIGAP
                     AKPIVEVGDEVKFGQKIGEQQGFVSCNVHSSVSGKVIAIEQHEVPGGSAQCVVIENDF
                     KEELHESVQPKGQLEDLSKEDIVGIIKEAGIVGMGGATFPNHVKVSPPPDSKAEVVIL
                     NGAECEPYLTADHRLMVENPEDVVFGLRALMKVLDVKKGFIGIETNKPDAIEAIQNVA
                     KDYSEIEVVGLQVKYPQGAEKQLIYACTGKEVPSGGLPIAAGAVVDNVATAAQIAKSI
                     KTGMPLVERITTITGSCIKEPKNLITKVGTLVSEIIEQCGGFKEDKKIGKVIMGGPMM
                     GIAQYTTEIATNKGSSGILCLDEEESRTPDIQNCLRCGRCTDVCPSFLQPLFISAYSL
                     KDDYDTAEYHRAMDCIECGSCSFICPARRPLLQSIRSAKREIGAKRRKQAAQK"
     gene            11256..12233
                     /locus_tag="KPD58_000863"
     CDS             11256..12233
                     /locus_tag="KPD58_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HBG0264647.1"
                     /translation="MENKLIVSSSPHVRSNEDTSYIMKQVIIALLPAAVAGVYFFRLN
                     ALSAMFFCILGTVGTEFLYQKFTKQKSTIGDFSAVVTGLLLAFNVPASLPWWMCLVGG
                     IFAILVVKMVFGGIGCNFVNPALAARAFLLASFPVAMTAWTQPGVNWIGKNLDAVTTA
                     TPLSFLKNGAAGLADLSSNGISLADMMIGNIGGCIGETSAILLLLGGVYLMYKGIINY
                     VIPVFYIATVFILTFLLGGFNITFAIYQLFAGGLMLGGFFMLTDYTTSPMTKKGQIIY
                     AVLAGLITTVIRMYGGYPEGVSYSILLVNCLAPLIDKFVRNRVFGEVAK"
     gene            12233..12802
                     /locus_tag="KPD58_000864"
     CDS             12233..12802
                     /locus_tag="KPD58_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HBG0264648.1"
                     /translation="MNSMVRLGGTLLAISAIAALALGATNQVTAPVIEQRNIQANNEL
                     RKAVLPEAKEFKEMKEDTYKGLGDGLIAEVYEGLDGSEVVGYTLKAKPSGYGGEIEVM
                     VGISSEGQVTGVDIGNMSETAGLGAKAKDDAFKGQYKGKTAEPLEVAKGSTTADNQIL
                     AISGATITSTAVTTGVNAAIDVFNSALNK"
     gene            12815..13408
                     /locus_tag="KPD58_000865"
     CDS             12815..13408
                     /locus_tag="KPD58_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HBG0264649.1"
                     /translation="MNLAKVFKNGLIDENPTFVQVIGMCPTLAVTTSAINGIGMGLST
                     AAVLICANLVISLIRKITPDKIRIPIFIVVIATFVTIVGMLLKAYVPALDKALGIYIP
                     LIVVNCLILARAESFAFKTGAMPSIVDGVGQGLGFTVALTIIGAVRELLGNGSLFGMT
                     LFGASFQPVLIFILPPGAFLTLGFLFAGFNKLRSKKA"
     gene            13423..13998
                     /gene="rsxA"
                     /locus_tag="KPD58_000866"
     CDS             13423..13998
                     /gene="rsxA"
                     /locus_tag="KPD58_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HBG0264650.1"
                     /translation="MNLILLFLSIVLVNNVITSQFLGICPFLGVSKKVDTAVGMGVAV
                     TFVLTLASIITYFIQILLVKTGTGFLQTIAFILVIASIVQFVEMVIQKMSPSLYQALG
                     VYLPLITTNCAVLGIALVNVQKSYNLVETIINGFGAGAGFTLAIVIFAGIRERLELAD
                     IPEAFKGFPITLISAGLMSIAFLGFTGLIKL"
     gene            14014..14985
                     /locus_tag="KPD58_000867"
     CDS             14014..14985
                     /locus_tag="KPD58_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HBG0264651.1"
                     /translation="MVILTAVLVLGIMGLIFGIVLDFASKKFAVEVDERVEAILGVLP
                     GANCGGCGFPGCGGLANAIVEGNAPVNGCPVGGADVGAKVGEIMGISAEAGEKQVAKV
                     ICKGTCSSAKDKYEYEGISDCRAANVLNSGAKMCKFGCLGLGTCKDACKFDAISIVDG
                     IAVIDEEKCVNCGKCKEVCPKGIIITKPESQEVVVECNSKEFGKAVKEKCTAGCIGCG
                     MCVKACKFDAIIFEDKIAKIDPNKCVGCMQCVAKCPTKVISGDITKKKKVTIDQELCV
                     GCTVCKKQCKFDAIEGELKEKHKVDADKCVGCHLCMEKCPKKAIKIL"
     gene            15205..15789
                     /gene="maf"
                     /locus_tag="KPD58_000868"
     CDS             15205..15789
                     /gene="maf"
                     /locus_tag="KPD58_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HBG0264652.1"
                     /translation="MNIILASASPRRKEILENTNVRFDIIKNEIDEIILEGETPKHLV
                     MRLAFEKSMSVASEHNEDVVIGADTVVVLDNAILGKPKDESCARDMLKRLSGREHQVI
                     TGISLINLCEDKKVIDYVISNVKFKTLSEQDIEDYLKTNESFDKAGAYGIQGYGALLV
                     EEIRGDYFNIVGLPISRLGDLLKKYFSINLFYGV"
     gene            15834..16493
                     /gene="radC"
                     /locus_tag="KPD58_000869"
     CDS             15834..16493
                     /gene="radC"
                     /locus_tag="KPD58_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBG0264653.1"
                     /translation="MAPEERPREKMLAKGVKSLSNAELLAILLRTGNKNKNAIELANY
                     IINRDIQGIRHLEDMTIEELCNIDGIGLSKSTQIKAALELGSRVASFKPIKYKIRNPW
                     DIQRYYMDSLRYLKKEVFKAVLLNTKNEIISDVDVSIGTLSSSLVHPREVFKEAIRRS
                     ASKIIVMHNHPSGSVEPSREDKNITSRLIKCGEIIGIEIIDHIIIGDGLYFSFKENMI
                     I"
     gene            16614..17624
                     /locus_tag="KPD58_000870"
     CDS             16614..17624
                     /locus_tag="KPD58_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HBG0264654.1"
                     /translation="MTKDMGIDLGTANTLVYIKGQGIVVREPSVVAIRDDSKEVLAVG
                     EEAKKMIGRTPGNIVAIRPMKDGVIADFDITQSMISYFIQKAADKKGVVSPRIAICVP
                     FGVTEVEKRAIEEAARQAGAKDAFLIEEPMAAAIGAGLKVEEPEGNMVVDIGGGTSEI
                     AVISLGGIVTAKSIRIGGDEFDESIVAYVKKEYNLMIGERTAENVKINIGSTFKDDEE
                     INMQIRGRDLISGLPKTIEICSTEVREALKEPVSSIVDAIKSTLERTPPELASDIMEN
                     GIMLTGGGALLRGLDKLITQETGMSVQIAETPLDCVALGTGKSVEDQEIFEKVLMMNT
                     KN"
     gene            17634..18527
                     /gene="mreC"
                     /locus_tag="KPD58_000871"
     CDS             17634..18527
                     /gene="mreC"
                     /locus_tag="KPD58_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBG0264655.1"
                     /translation="MALRFDKNKNEKKRINVKVIATGVVAITLIGIVGISIGKFSSGS
                     PVNLGVASDAITSVGKGINDGFSFIKNGFKDVANFKDNSKKVKKLEEENEKLKKNMIA
                     LNAKLDKTESLEELKKTLNFVQEEYKATSISASVVGKNDGNWYESFVIGAGKNSGVKK
                     ESIVMNGSGLVGIVYEVSNNYSKAISLLDSKASVSFKLAKDANAKGTITQNTTLDNKD
                     SYNSKGYLQGYMFDSSYNVIQGDIITTSGLGFFPDGIPIGEVEKVVDDKDKSLKYVVV
                     KPYVDFKNINDVVVIEPRNIG"
     gene            18542..19030
                     /gene="mreD"
                     /locus_tag="KPD58_000872"
     CDS             18542..19030
                     /gene="mreD"
                     /locus_tag="KPD58_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBG0264656.1"
                     /translation="MKKVLLCLLGIVLMTIENSITNYIDIFGISFNLVLIYVTIISLY
                     LDELEGGIIAAIIGLIKDITVGGIFGVNALILFIIAYAIGYMRDQLYKGSYIITFVLI
                     FIGSLIDSIVNIGTSSIIYQSYNISTLFVKGLLVAPIVNSLIGLVIYRLSKRAVLKLK
                     ED"
     gene            19042..22020
                     /locus_tag="KPD58_000873"
     CDS             19042..22020
                     /locus_tag="KPD58_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBG0264657.1"
                     /translation="MEENKQVDRLKIIKIVMGVIFLAILVKIIYMTTFKYEYYNELAE
                     NKTYKKLAIEAPRGEIKDRYGRLLAGNKNLFTVQVSGNDINKKDANKHSRANEISLKL
                     INLLERNGEEYVDEFPIYVENGKYYYTYDRDIREYKSENGIPNDYNAKESFYYLVDKL
                     ISAGILSQEDKRLDATRLQAKLNENGYYPPILVSKWMFTAERDKRDWLASYKIKETKL
                     SAKEAFEKVRNSDALEIDKSLSDEDARKIMVVRDLIKSKGYSQYNPVTIAKDVGETTI
                     AQIEESAMDLVGVSIAVEPVRYYPNGSLASHMLGYVGKMPSTQIESYLQKGYETGDMV
                     GLAGVEKSNESRLRGTDGYKMVKVDALGRISKEIESKKPKSGDTVYLTLDKDLQEVSD
                     NALKQIIEVASKGGTFKSKFGDKPISAYAGKAQSAALIAIDVKNGEVLASSSYPNYDP
                     NKFAKGISTEDYKALQPKNPNDLLAGSPLLNLVTQGEFQPGSSFKMVTSMAALENGLD
                     PNFTINDPGVIMLGKKSFGDYVWNHGRGNHGMTNLYKAIQESCNIYMATIGTGKTWPD
                     GKSIGIDMNANKILEYAKLFGLDQNTGLQDEVEERAGKVPSTEDKLKSTQALLKSNLE
                     REMANDFVDITREKNPKEYEKRINEIVSWAAEKKTPGRVETMNRLKKLDVKEDRIEEV
                     ADLAVFSYFNFAKWSTADTFNLAIGQGENAYTPAQISRYVAAIANGGNLVELSVVDRA
                     VSSDYSSVKINDQKKVEKIPFKNPDNLKELTKGMKLVARQGTAKSAFADFPIDVAAKT
                     GTAEKSGKIPTDNEYEYLKSHMSSYNVNLNDAIKLADKMKAEKEKELSLAKEKEIKKK
                     LENKDLKDEERKKLEEELEDGVKVRLEDTDKVNSSYLRKAIKELNPKITDDQIDRFKQ
                     DYGSFTWTVAFAPADDPEIAVVCVIPQGDSSVFSLLPTREVIGTYMGLEPTNSKNDNK
                     TNDVNNSSDENINFESQINR"
     gene            22118..22801
                     /locus_tag="KPD58_000874"
     CDS             22118..22801
                     /locus_tag="KPD58_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HBG0264658.1"
                     /translation="MSLREICSQELVEFKGNKRGIIVNIKREAPFEEIQEKIINKLEA
                     YVGFFNGAKISKINSDCLTDMEILELKEGITSRFDVEFVEDQKIEENSNFPTKYVNTL
                     RSGENIEFEGDVVILNDMKPGSKVLSKSNTVVMGDINAGAKVVAGGNVFVMGKIEGFV
                     HAGAEGNEFAYVVAGNLNPKILQIADNIAEAPDDEENYESESEISPEIAFVSNGRIVI
                     ESYLSKLDK"
     gene            22825..23622
                     /gene="minD"
                     /locus_tag="KPD58_000875"
     CDS             22825..23622
                     /gene="minD"
                     /locus_tag="KPD58_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HBG0264659.1"
                     /translation="MSEVIVITSGKGGVGKTTTAANLGTALSLENKKTVVVDADIGLR
                     NLDVVMGLENRIVYDIVDVVEGTCRLKQALIKDKRFDNLYLLPAAQTRDKNAVSVEQM
                     IDLCEKLKESFEYIIIDCPAGIEQGFKNAVAGADRAIVVTNPEISAVRDADRIIGLLE
                     ANEIKEIRLVINRIRNDMVKRGDMMDKQDIIEILAIDLLGLVPDDESIIISTNKGEPA
                     ILDSKSLAGQAYKNIAKRILNEEVPLLDLEVEDGFFGRLKKMFSMAK"
     gene            23640..23924
                     /gene="minE"
                     /locus_tag="KPD58_000876"
     CDS             23640..23924
                     /gene="minE"
                     /locus_tag="KPD58_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HBG0264660.1"
                     /translation="MLDLFRVFSNEAKTSKSVAKERLKLVLVHDRVDCSPQLLEMIKT
                     DILKVIANYAEIEDDGLEIKMSKCRGEHDDKPVSALVANIPLKNIKDRCM"
     gene            24009..25139
                     /gene="rodA"
                     /locus_tag="KPD58_000877"
     CDS             24009..25139
                     /gene="rodA"
                     /locus_tag="KPD58_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HBG0264661.1"
                     /translation="MKKININYKSTIKLIKQLDWKLIVTVLAIFIFGLVILSSATHAN
                     STGSYNQLIKQGLAFVLGIGMIIVILFFDYNLLGRYYKALYIISLILLAIVLLPGIGT
                     VKGGARSWINLGPLDLQTSEIVKLTFVLSYAKILESKKDKLNTLKEVMPVVVYSLPFI
                     GLLIAQPDLGTGIVFCCMIFAMLFTAGLSSKLIKRGIIILLVSMPLMYLMMADHQKVR
                     IEAFLNPEDVTLKGNYQVMQSLIAIGSGGVTGKGLYNGSQNQEDFLPVQDSDFIFAVV
                     GEELGVIGMAVLIILFMIFLLRLLAIARDAKDFYGTLIVVGVMGMFGYQIIQNIGMTV
                     ALIPVTGVTLPFVSYGGSSLLTSLANLGLVLNVCMRRKKINF"
     gene            25180..25593
                     /locus_tag="KPD58_000878"
     CDS             25180..25593
                     /locus_tag="KPD58_000878"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBG0264662.1"
                     /translation="MNIALVAHDQMKNTMVGFCIGYESILKKYGLYATGTTGKRIMDE
                     TELNINRLASGPLGGDQQIGSLIVTQEIDLVIFLRDPLTSQAHETDIQALIRLCDVYH
                     VPIATNLASAEIFIKALDRGELSWREVRKSKSQRI"
     gene            26078..26407
                     /locus_tag="KPD58_000879"
     CDS             26078..26407
                     /locus_tag="KPD58_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0264663.1"
                     /translation="MQLNKEVLKGYIDILIVSILEKKDCYGYEIAKQVRERSEFELKE
                     GTMYLALKRMESKNLIKSYYSNEQSSGGRRKYYNLTNEGKDFLEIKKQEWRFIKKVMN
                     QFLGEVD"
     gene            26411..27202
                     /locus_tag="KPD58_000880"
     CDS             26411..27202
                     /locus_tag="KPD58_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264664.1"
                     /translation="MKLIDEYLDKLYKKCDNKSTIELKQEMRCHLIESANEFKLEGLD
                     EEEACKKAIERFDDGDEMQYELCNIIKELSLSLDRHKSIVMGFKKVLGYISIIAFLIS
                     GFMWYYNNSLQHNMYNLGKELDGEIKQLAERHDMTKIGEYKLELEKILDKDKYSKVKA
                     LRLYVIDMKDGNTNLSSSGLNANMVYEREADYNNISNFIQHLGYNGKDFLDKNGNIVN
                     PDIFLEYFFYFESEMLIPVAFAFGLLCIIAYFILRFKISLIKNNN"
     gene            27766..28848
                     /locus_tag="KPD58_000881"
     CDS             27766..28848
                     /locus_tag="KPD58_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBG0264665.1"
                     /translation="MKKIRKLATKLLIAMMVLTSCMSVTVYGMTAKDVTAKTPKSYVA
                     GTNSIYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKIRVAYNHIKNNTTYVNWNA
                     AEGANTAYTLVTKKGACSGMCRSLKALCDAMGIECYYVHSTTNDHQWNLIRFEDGRLY
                     HVDIDANKSAGADIIYKSLTHPLPFDKKAYPAVGEVVKTQNPTPTVKKPTPAKPSAPV
                     GPGNHTMIADQQGAILDEVYGNNLRPKIEMYEGVPCSLINSSINTIKFTSKNSNLDPS
                     KHTVTVIGFMNTPYGMVALAPDGVPDEYPFEYDKPFSLDFTGKNLSAAELSKKCKENN
                     VIIKVEIGETASNSGRGMVAYARYVN"
     gene            complement(29060..30253)
                     /locus_tag="KPD58_000882"
     CDS             complement(29060..30253)
                     /locus_tag="KPD58_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HBG0264666.1"
                     /translation="MSKVFEVKKDIYFTGVVDEGLKVFDIIMETEFGTTYNSYLIKDE
                     KTVLFDTVKANFKDEFLSNLSEVTDIAKIDYVVIHHTEPDHAGSLKYLLDINPNIEVY
                     CTKAAKLYLDGQINRPFNCHVIKDGEILNIGKRNLRFITAPFLHWVDTMFTYIEEDKT
                     LLTCDAFGCHFASVDAEVVNSEDYLKSAKHYYDCIVKPFAKHVLSAVDKVVGLNIEFD
                     TILTSHGPMLTKDPMAAVKRYVEWSTEAVNTTNQNQVSIFYLSAYSNTLEMAKKIKEG
                     LDKEGAKAELYDLEDMTLTEMHDTLVVSKVILLGSPTINKTMVKPMWDLFSVIDPMAN
                     QGKIAGVFGSFGWSGEGITMAETLLKSMSFKMPVESLKKKFFPSEETLKECMAFGAEF
                     AKLVK"
BASE COUNT        11932 a         3402 c         5767 g         9242 t
ORIGIN      
        1 ggagtgatat aatttggaaa aaaaattaat tataatagat ggaaattcaa taataaatag
       61 agctttttat gctcttccag aaatgaataa taaagaaggg ttaaagacca atgctatata
      121 tgggtttact acaatgctat ttaaaatgat agatatatat aaaccaacac atataagtgt
      181 agcttttgac agaaaagcac ctacatttag gcatttagaa tataaagatt ataaggctgg
      241 aagaaagggt atgccagatg aattggcaga acaattacag ccattaaaag accttttaga
      301 taaatttaga ataaatagac tagaaataga tggttatgag gcagatgata ttataggtac
      361 tgtttctaaa aaagcagaag aaaatggata taaggtatac atagtaactg gagataaaga
      421 tgctattcaa cttgcttcag ataatacaac tacccttata acgaaaaaag gggttggaga
      481 agtagaagaa tacaacttta attctgtaat tgaaaaatat gagatgactc caacacaatt
      541 tatcgattta aagggtctta tgggagacaa gtctgataat atacctggag tacctggaat
      601 aggtgaaaaa acaggtataa agcttataaa agagttttct agtatagaga atctaataga
      661 gaatacagat aagcttaaag gtagtgtaaa aaagaaaata gaggaaaaca aagaaatagc
      721 tattcaaagt aaaagacttg caacaattat aagagatgtg ccaatagaag tggatttaga
      781 tagtatgatt tttggcgatt atgataaaga tgaactttta gataaattta gatactttgg
      841 atttacaagt ctcttatcaa gattagttga tttggtcgat tcagatgaca cagaacacgt
      901 agaagaaaaa atagaaatat taaaattaaa agatatagaa aaatttatag atgaagtaaa
      961 caaaaaagga caagttattt taaaaactgt tagaggacaa ggtaatatac ttgaaaaaaa
     1021 tataatatat atttttataa gtgtagatgg agaaaaaata tattatgtag attcaaatga
     1081 agtgtcaaag ttagatagta tattttcaaa taatgaaatt aaaaagtatg gatataattt
     1141 aaaagatgat tatatatctt taaaaccata taatataaac ttagaaaata tgtattttga
     1201 tataacaata gctgagtact taatagattc aacttcatct acctcatatg attgtagtgc
     1261 aatagctatg aagtatttat caaaaaaggt taaatcaaaa gaagaattat taggaaaggg
     1321 tgttaaatct aaaaactatg aggatttaga atttgaggat ttagcagagt atatgggtca
     1381 aataatatgc acagttaaag aaacagttcc tatgatggaa agaagtctaa tggatatgag
     1441 tatggatggt cttttttatc atgtagagat gcctttagta gaagtcttag gtcatatgga
     1501 gtatgaaggt ataaaagtag acaaaagtat attggatgaa ttgagtgttg aatttaaaga
     1561 aataatagct acattagaaa aggaaattta tgaattatca ggagagccat tcaatataaa
     1621 ttcaccaaaa caattgggtg ttatattatt tgaaaaatta gagcttccaa taattaaaaa
     1681 aactaaaact ggatattcaa ctaatgcaga tgttttagaa aaattaagag ataagcatcc
     1741 aataatagat aagataactg agtatagaca aatagtaaag ttaaattcta cttacgttga
     1801 aggactttta aatataataa atccagaaag taatagaata cattcatcat ttaatcaaac
     1861 tataacgact acaggaagaa tttcatctac agaaccaaat cttcaaaata tacctataaa
     1921 aatggaaatg ggtagaaaaa taagaaaagt gtttattgca gatgataact gtaagttggt
     1981 tgatgccgat tactcacaag tagagctaag agttcttgca catatgagtc aggatgaaaa
     2041 tatgatagaa gcatttaaac atcatgaaga tattcatacc aaaacagctt cacaagtttt
     2101 taatgttcct atggaagatg ttactagtga attgagaggt gcagctaaag ctgtaaactt
     2161 tggtattata tatggtaaaa gtgactttgg tctagctgat gatttaaata tacctgttcc
     2221 aaaggcgaaa gaatatatcg aaagttattt tgctaaatac cataaaataa aagagtttat
     2281 ggacacaaca gtagaaaagg ctagtgagga tggatatgct gtaacaattt taaacagaag
     2341 aagatatata ccagaaataa agtcaagcaa ttttatggaa agaaatagag gaaagagatt
     2401 tgcaatgaat gcaccaatac aaggaagtgc agcagatata ataaaaattg ctatgataaa
     2461 tgttcataaa aaattggaag agaacaattt gaaatcaaaa cttatactac aagttcacga
     2521 cgagttgata gttgaagcaa ttgatgatga aatagatata gttaagaaaa tagtaaaaga
     2581 agaaatggaa aatgctgtaa atatggatgt tcacttagat gtagatttaa atgtaggtag
     2641 ttcttggtat gagacaaagt aggtgatttt atgttgatat taggacttac aggaggaata
     2701 ggctgtggaa aaagcagcct atcaaatata tttagaaatc ttaacattcc tatagtggat
     2761 gctgatatta tatccaggaa gatatttgag gataagttgt tattggaaaa agtatttgtc
     2821 cattttgggc aaagtataaa aaatgatgat ggaactctaa atagaaaagc tcttgggaaa
     2881 atagttttta gtgatgaaga aaaacttaag gaattaaata acttgactca tccacgaata
     2941 agagaaaaaa taataagtga aatagaaaaa ttgagaaaaa aaggcgaaaa tatagtagtg
     3001 ttagatgcgg caatattggt agaaagtggt tttttagata tggttgacaa gctattagta
     3061 gttacttgta aacaagaagt acaaattagt agaatacaaa agagagataa ttgcagtgag
     3121 caggaagcac ttagtaggat aaattcacag atgagtcagg aagaaaaatc caaatatggt
     3181 gattatataa tagataattc gggaacaata actgaattag aaagtaaagc acataaattt
     3241 attgagtaca tgaaggagaa ttggcgtgaa tagtaagaaa gtattgattc tatctatttt
     3301 tataatctta tttggggcac tattaatgga aagcaaagta atacataaat ttttatatcc
     3361 taaaaaatat tcagagtatg tagaaaagta ttcgaaagaa tttaatttag atgaaaatat
     3421 agtttacagt gttattaaag ccgaaagtaa gtttaatagt tctgctgttt caaaaaagga
     3481 agcaaaagga ttaatgcaaa tattagacat aactagagat tggggagcag aggaactaaa
     3541 tttaaaaaat gtggatattt tcgacccaga gactaatata agacttggct gttggtattt
     3601 aagtaagtta tacaaagaat ttggtaaatt agatttagtg atagctgcat ataatggtgg
     3661 ttcaggtaat gtgaaaaaat ggttagaaaa taatgaatat agtaaagatg gcgaaaatct
     3721 acatgatata ccttttaagc aaacttcaaa atatgtagaa aaagtaaaaa ataattacga
     3781 acattataat aagatatatg gcaagaaagg aaaaaactaa tgaagagaat aaaagtctta
     3841 acagtcgttt tagctataac tcttatggta gctggttgta gtaatactaa aacaaaacaa
     3901 agtagtagtg attcaagttt acaaagtcaa agttcgatag atgctaagta tataaactta
     3961 acaatggtaa ctccaaaaac tataaatcct ataactaata ctgataagtc tgttggttat
     4021 ataatgaatt tagtttatga tagtttattt actatagatg agaactacaa tgtaattcct
     4081 caattagtta aggagtataa tatagctcag gatggaatga gtatagatat aaagctaaaa
     4141 gatgcaaagt ggcatgatgg aaaaaatgtt acttcaaatg atgtaaacta tactattggt
     4201 ttaattcaaa aaagtgcaga tagtccatat aatgaattta ctaaaaatat agcttctgtg
     4261 aatattaaat cagataaaga ttttacaata aaatttaaag ctagatatgc tttttcaatt
     4321 gatagtttaa tttttccaat tgtatctcaa aatcatttag attcaaaaga tgtaaatgac
     4381 aagaataata atatgatagg aaatgggaaa tataaaatag aatcatatac agaaagagaa
     4441 ggaatggttt tatctgtaaa caaagattac tatgaagaag ttccaaaaac catgaagaac
     4501 ataaaagtag gtatggtgcc taatgaagat gcaaggacat ctatggttat ggctcttgat
     4561 agtgatataa ccaatgttac attaaatgat ttaagtaagt ttcaagaaaa agagtttaat
     4621 ataacaaaat atcaaggaag agattatgag tgtgtattat ttaattataa taatcctttt
     4681 ttcaaagatg ttaattttag aaaagcaatt gcacactcta tagataaaga tagaattatt
     4741 agtgaaggtt atatggatga tgctactcca gttaattttc cacttaattc aaaatcaaaa
     4801 tactataata gtgaaatgaa ggatttagag tttaataaag ataaggcgat agagtgtctt
     4861 gctaaggtag aatatgcaaa tgtaaactca gtaaatcaaa atgacaataa agtaaaaaat
     4921 cagaaaaaat cagctaaaaa gaactcaaag aagcttacgc cagaagaaga agcaaaagct
     4981 aaaaaagcag aagaagatag aatcaaaaaa gaagctgaag agaaaaaaaa tagagaaaaa
     5041 gaggaagtaa agaaaagtct ttctgaaatg aacttaaaga taatagtaaa cagagataat
     5101 agtgagagaa taaaaactgc aaacataata aatgaaaact taaaagctat aggaataaat
     5161 agtacagtaa atcagctgtc agataaggat atggaaaatg ctcttaattc taaaaattat
     5221 gatttagcat tagttggatg gaaactatca attataccag atgcaagtag tattatagcg
     5281 agtagtggat atactgatga taagttaaat ggatatatgt ctgcacttac aagttctacg
     5341 tctgaaattg aaacaaaaaa agcatacaaa gatgtacaga catatataaa agataatgca
     5401 acatttataa gtttagctgt gagaaacaac tatattgtaa gtaatagtag attaaaaggt
     5461 aaaattactc caaatgattt tgatgtatat gagggaatat ctaatttaga tataaaatcg
     5521 aatgaatcaa attaaatgat taatttctat ttaaaaagta taaactatat gatagaatac
     5581 ccttataggg tataaggagg taattaaatg aagaagaaac tattagtaga aggtatgagt
     5641 tgtggtcatt gtgtaaacca tcttaaaact gcattaacag aagatataga tggtattgaa
     5701 gttttagatg tagatttaga aaataagtgt gcatctgtag atatgaaaga tgatgtatct
     5761 attgaaaaat taaaagaagt gatagcagaa ttaggatttg aactaaaggg aattgaatag
     5821 aaattgataa actaaaaaaa gacctctatt gggggagtaa tagaggtcta aaaatggggg
     5881 agattgagta tgtttgattt tacattacta ttatctcccc ttttaaatta atatatacat
     5941 aaaaagtaaa aaaattttgt aatgactcac atttcttgag ctttttgaat atactataaa
     6001 tagctaaggg agggagtatc atggaatcta ggggatataa agaggattta aaaaaaatga
     6061 tacttgatat ggcccacaaa gaacttgaga gttatgtatc acaaaaagga gcaaaaaaat
     6121 actttgaagg taacttggat aagattatta ataataatat aaaaaaattc aatcaaagta
     6181 ttggtcttaa tagagtatat ttaaaaatgt taaaaaagtg tgcatatcaa gaaagtgtaa
     6241 cttcaatatc aagaactata aagaaagaag cattatttaa atcaaaaaaa gagctatttg
     6301 ctttttcaga gtacttaaaa cttgatatta acaagcgact ttcatataat caaattttaa
     6361 gaaaaatatc aagatatata tactacaata gaagttcata tgctcaaaaa tacgttgttt
     6421 tcaaaagagg agatgaagaa tatcttttag aaccagagaa aataaaagat gaattaatat
     6481 caagctatag gtcaaagact agagaagata tgaggtcaat agcaaaactt ttggatatag
     6541 aagtagaaga tgattataat gctgaagata ttagaaaaaa agtaataaat tatattatta
     6601 aagaaaaact taaaagttag agttttaaag aataagttct gccaatttgc atgattaagt
     6661 ataacttggg tataaatttt ttatgcattt gaaagagctt attctttttt tgacatattc
     6721 ttttaaataa tttaccatga tttggtaatt ggtcatcatc attcaaattt ttaacttaat
     6781 atgatttaaa taaatttata ataatgatat aatataaaaa aaataaaaat tggagagtga
     6841 tttcaaaatg aagtttaatt tttgtcataa caatttcaat gttactaatt tagaaaaaag
     6901 tttagatttt tataaaaaag ctttagggct taaagaggta aagcgcaagg aagcagaaga
     6961 tggaagtttt atattagttt atttaggaga tggcattact agtcatacat tagaattaac
     7021 ttggcttaga gattgggata gaccatataa tttaggagac aatgaatttc atctggcttt
     7081 agaagttgag gagtttgatg aggcaaaaaa acttcataaa gatttagatt gcatatgttt
     7141 tgaaaatgaa agtatgggaa tttattttat agcggaccca gataattatt ggattgaaat
     7201 acttcctaaa aatcactaat tttaaggata tgtttataat ttctttacaa ttaataagaa
     7261 aataagttat cattaaattg tagggactaa aaatggataa taatgttatg aatttatgac
     7321 tataaaaatt tgttgggggg agtgatagta gtgaaaaaag ctatagcagc tttaggaatt
     7381 ggggcagtag cagtatctgt tagctccata aatgcaagtg ctcttgaaaa gggaacagta
     7441 acagcaagtg ccttgaatat aagaagtgga ccaagttctg attgcgataa ggtagcaaaa
     7501 ttatacaagg gaaaaactgt agagatttta gaaaagtcta atggatggta taaagtaagg
     7561 gtatcgagtt ctgttgttgg atggggaagt gcaaaataca tatcaacaag tggttcgtct
     7621 gaaggtacat caagtcaaaa taatccaact tcaagtggga caactattag tggaaatggt
     7681 aaagtaaatg tgagttctag actgaatgta agaagtggtg caggtactaa ctattcatta
     7741 gttggtaaag caaataatgg agatatagta aagttactag aacaaagcaa tggatggtat
     7801 aaaataaaac tatcaaatgg agttactgga tgggctagta gccaatatat ttctaaaaca
     7861 tcagaagatg ttggaacgaa caattctagt aattcaaata gtactaataa ttctgataaa
     7921 aaaccatcta gcgaagaatc aattgaaggc aagaatggta aggtaacatc tgctgtgagt
     7981 ttaaatgtaa gaagtggtcc tgggacaagc tattctataa taggaaaatt aaatggtgga
     8041 gatgttgttg aattaaaagc taaaagtaat ggttggtata aggtaaagtt atcgagtgga
     8101 acaattggat gggtgagtgc aagttatatt tctgagacta atgaaggtac aaaagaaaaa
     8161 ccaaattcat catctaacca aaattctcaa cctaatagta actcaaagcc atcatttact
     8221 ggaaattcag ataaatctac agcaaaaggt tcaacaatcg tagattttgc atatactctt
     8281 attggaatac catatcaatg gggagcttca gggcctgata aatttgactg ttctggattt
     8341 acacagtatg tttttaaaca ttctgtaggc gtttctattc ctagagtttc tagagaacag
     8401 gctaattttg gaagtgcaat ttctatgggg aattatgcac caggagattt agtttatttt
     8461 gacactgatg gtgatggaac aactaatcat gtaggtatat atgtaggaaa tagtaaattt
     8521 atccattgta gtggtactca gactaatcct aataaggtta aagtggataa tttaactagt
     8581 tcatattggt ctaaagtatt acttggtgca agaagatttg tttagtaagt acaatttctt
     8641 attaaaaaat tagaagaaat aattatttat attcataaat tgtgactctt aaaaatatac
     8701 tttattaata atataatttt gggagggaca aactaatgaa tagaagaaaa acgatataca
     8761 gaggatttaa taataataga cataagattg atttacgtaa atatgttata acgattgctt
     8821 gcttgtgtct aattggctat tattcttata caaaaataaa agatagcaag atattggagt
     8881 atgtatcggc taaaatacca tttctaaata attcatcaga tataacttat aaagatatat
     8941 ctgatgaatt aaattcaata aaaaaaggga aaaaatctaa atcacaaacc aattcagatg
     9001 ataaacaaga aactaatcca gaaaaagcgg taaataacac taaagagcca gaagaagtca
     9061 aattagctac tatagaaggc tgggatatgt atactattca agttgctgca atagataata
     9121 atgatgattt gaaaaaaata cagacatcat tagttaataa tgacatacct ttttcagtta
     9181 tggagaagga tggagtaaaa aaaatacaga cctattcctc ttttgatgaa aatgatgtta
     9241 gaaagcagat aagcagtgta agaaaggtgt ttccagatgc atttttatca cacttagatg
     9301 cacctatgtt gtctttagag tatacaagta actacgctta tatagagagt atatctaaag
     9361 aattaaataa attaataaca aactttaaag aagaatcaag tttttggtcg aatgctgaaa
     9421 ataatgtaga tatggaaaaa tataatacta ttttgacaaa tagaaaggca atatcacaaa
     9481 atatatcaaa agaagctgaa aagattgatt attctgaaat gcgtttattt aaagataatt
     9541 taatagagta tgtaaagaat gtgaatgaaa aaattgatac agcttcaaaa gcagcaaatg
     9601 aagaaaaata tagtgtaagt aaaagtctgc ttttatcatc catgcaggag tactctatgt
     9661 ttataaattc tataaaataa aatacagaaa atttggactt tagaaagcta tatgaaaagg
     9721 attgtttaat taagcaatcc ttttcctcaa tttgacttaa tatagaggtg ttgatttata
     9781 aaataaatta taaaaatacg gatatttttt taacaattgt taagattttt taatatatat
     9841 agtatactta tagaaggcag aaaaaatgtt tttttataga taagggggta aaacaatgaa
     9901 actcttaact tttaagggag ggatacatcc tccatataga aaagagtact ctaacacaaa
     9961 ggcacttgaa aaagctcaag ctccaaagat agtttacatt cctcttcagc aacatatagg
    10021 ggcaccagct aaacctattg ttgaagttgg tgatgaagta aagtttgggc aaaaaatagg
    10081 tgaacaacaa ggttttgttt catgtaacgt tcattcttca gtatcaggta aagttattgc
    10141 tattgaacaa catgaagtac caggaggttc agctcaatgt gtagttatcg aaaatgactt
    10201 taaggaagaa ttacatgaga gtgttcaacc aaaaggtcag ttagaggatt tgagcaaaga
    10261 agatatagta ggtatcataa aagaggcagg aatagtaggt atgggtggag ctacattccc
    10321 taaccatgta aaagtatctc ctccaccaga tagcaaggca gaagtagtta tattaaatgg
    10381 tgcagaatgt gaaccatact taactgcaga ccacagatta atggttgaaa atccagaaga
    10441 tgttgtgttt ggtttaagag cattaatgaa ggtattagat gtcaaaaaag gatttatagg
    10501 aattgaaacg aataaaccag atgctataga agcaattcaa aatgtagcta aagactatag
    10561 cgaaatcgaa gttgttggcc ttcaagtgaa atatccacaa ggagcagaga aacaacttat
    10621 ttatgcgtgt acaggcaaag aagttccatc tggtggatta ccaatagcag caggtgcagt
    10681 tgttgacaac gttgccacag cagcacagat agctaagtct attaaaacag gtatgccttt
    10741 ggttgaaaga atcactacaa taacaggtag ttgcatcaaa gagccaaaga acctaataac
    10801 aaaagttgga acattagttt cagaaataat agaacaatgt ggtggattta aagaagataa
    10861 gaagattggt aaggttataa tgggtggacc tatgatggga atagctcaat atactactga
    10921 aatagcgact aacaaaggtt cttcaggaat tttatgtttg gatgaggaag aatcacgtac
    10981 tccagatatt caaaactgtt taagatgtgg aagatgtaca gatgtatgtc catcattctt
    11041 acaaccactt ttcataagtg catattcttt aaaagatgac tatgacacag ctgaatatca
    11101 tagagcaatg gattgtatag aatgtgggtc ttgctcattt atttgtccag caagaagacc
    11161 attacttcaa tctataagat cagcaaagag agaaatagga gcaaagagaa gaaagcaagc
    11221 tgctcagaaa taaagaaagt taagggggaa aaacgatgga aaataagttg atagtatcat
    11281 cttctcctca tgtgagaagt aatgaagata cttcatatat aatgaaacaa gttattatag
    11341 cactccttcc agcagcagta gcaggagtat acttctttag acttaatgct ttgagtgcta
    11401 tgtttttttg tatacttggt acagtaggta ctgaattttt atatcaaaaa tttacgaagc
    11461 aaaaaagtac tataggagat ttttcagctg ttgtaacagg attattatta gcatttaacg
    11521 taccagcatc acttccttgg tggatgtgtc tagtaggagg aatatttgca atattagtag
    11581 ttaaaatggt atttggtgga attggatgta actttgtcaa tccagcactt gctgcaagag
    11641 catttttatt agcatcattc ccagtagcaa tgactgcttg gactcaacca ggtgttaact
    11701 ggataggtaa aaatttagat gcagttacta cagcaactcc attaagcttt ttgaaaaatg
    11761 gagcagcagg actggcagat ctttctagca atggaattag tcttgctgac atgatgatag
    11821 ggaatattgg tggatgtata ggtgagacat cagcaatatt attattatta ggtggagtat
    11881 acctaatgta taaaggcata ataaattatg ttataccagt attttacata gcaactgtat
    11941 ttatattaac attcctttta ggtggattta atataacttt cgcaatatat caactgtttg
    12001 ctggaggact tatgttaggt ggattcttca tgcttacgga ttatacaact tcgcctatga
    12061 ctaaaaaagg tcaaataata tacgctgtat tagctggtct tataacaact gttataagaa
    12121 tgtatggtgg atatccagaa ggtgtatctt actcaatact attagtgaac tgtcttgctc
    12181 cacttataga taagtttgtt agaaatagag tatttgggga ggtggctaaa taatgaacag
    12241 tatggtgaga ttaggtggta ctttacttgc tataagtgca atagctgcat tagctcttgg
    12301 agctactaat caggttacag ctccagtaat agaacaaaga aatatacaag caaataatga
    12361 acttagaaaa gcagtccttc cagaagcaaa agaatttaaa gaaatgaagg aagatactta
    12421 caaaggcctt ggagatggtc ttatagctga agtgtatgaa ggtttagatg gttctgaagt
    12481 ggttggatat actttaaaag ctaaaccaag tggatatggt ggagaaattg aagttatggt
    12541 aggtatatca tctgaaggtc aagttacagg tgttgacatt ggtaatatgt cagaaacagc
    12601 aggtcttgga gcaaaagcta aggatgatgc atttaagggt caatacaaag gaaaaactgc
    12661 tgagcctctt gaagtagcaa aaggaagtac aacagcagat aatcaaatat tagcaatatc
    12721 aggtgcaact attacatcta cagcagttac tacaggagta aatgctgcta tagatgtttt
    12781 caatagtgct cttaataaat aaggagggtt aattatgaat ttagctaaag tatttaaaaa
    12841 tgggctaata gatgagaacc caacatttgt tcaagttata ggtatgtgtc caacactagc
    12901 cgttacaact tcagcaataa atggaattgg tatgggtctt tcaacagcag cagttttaat
    12961 atgtgcaaat ttagttatat ctttaataag aaaaattaca ccagataaga taagaatacc
    13021 tatatttata gtagttatag ctacatttgt tacaatagta ggtatgcttt taaaagcata
    13081 tgttcctgct cttgataagg ctcttggtat atatatacca ttaatagttg ttaactgttt
    13141 aatattagct cgtgcagaaa gttttgcttt caaaacagga gctatgcctt caatagttga
    13201 tggtgtaggt caaggtcttg gatttactgt tgcacttaca ataataggtg cagtaagaga
    13261 attacttgga aatggtagtt tatttggaat gacattattt ggagcatctt tccaaccagt
    13321 attaatattt atattaccac caggtgcatt cttaacatta ggattcttat ttgcaggatt
    13381 taataaatta agaagtaaaa aagcatagtg gaggtgtaat aaatgaattt aatactttta
    13441 tttttaagta tcgttcttgt taataacgtt ataacatctc aattcttggg tatatgtcct
    13501 ttcttgggag tttctaagaa ggttgataca gcagtaggga tgggagttgc agttacgttc
    13561 gttttaactc tagcttcaat tataacttat tttatacaga ttttattagt aaaaactgga
    13621 actggattct tacaaacaat agcttttata ttggttatag cttctatagt tcagttcgtt
    13681 gaaatggtaa ttcaaaagat gagtccatct ttatatcaag cattaggagt ttatttacct
    13741 cttataacta caaactgtgc agttcttggt atagctctag ttaatgttca aaaaagttat
    13801 aatttagtag aaacaataat aaacggattt ggagccggag ctggatttac tttagcaata
    13861 gtaatattcg caggtataag agaaagacta gaattagctg atataccaga agcatttaaa
    13921 ggtttcccta taacgcttat atcagcaggt ttaatgtcaa tagctttctt aggatttaca
    13981 ggacttataa agctataaaa aagaggggtg aaaatggtga tacttacagc tgtattagtt
    14041 ttaggaatta tgggcttaat cttcgggata gtactagatt ttgcatctaa aaagtttgct
    14101 gtagaagtag acgagagagt ggaagctata ctaggagtac ttccaggagc caactgtgga
    14161 ggttgtggat ttccaggttg tggaggtctt gcaaatgcga tagttgaagg caatgcacca
    14221 gttaatggtt gccctgtagg tggagcagat gttggtgcaa aagttggaga aataatggga
    14281 ataagtgctg aagcaggtga gaaacaagta gcaaaagtta tttgtaaagg aacttgttca
    14341 agtgcaaaag ataaatatga gtatgaagga atatcagatt gtagagcagc aaatgtttta
    14401 aactcaggtg ctaagatgtg taaatttgga tgtttaggtc ttggaacatg taaagatgct
    14461 tgtaaatttg atgctatttc tatagttgat ggaatagcag taatagatga agaaaagtgt
    14521 gtaaattgtg gaaaatgtaa agaagtttgt cctaagggta taataatcac aaaaccagaa
    14581 agtcaagaag tagttgtaga gtgtaatagc aaagagtttg gtaaagctgt taaagaaaaa
    14641 tgtactgctg gatgtattgg ctgtggaatg tgtgtaaaag cttgtaaatt tgatgcaata
    14701 atatttgaag acaaaatagc taagatagac cctaataagt gtgtaggttg tatgcaatgt
    14761 gtagcaaaat gccctacaaa agtaatatca ggtgacataa ctaagaaaaa gaaagttact
    14821 attgaccaag aactttgtgt tggatgtaca gtgtgcaaaa aacaatgtaa gtttgatgca
    14881 atagagggag aattaaaaga aaaacataag gtagatgcag ataaatgtgt aggatgccat
    14941 ttatgtatgg aaaaatgtcc aaagaaagct attaaaattt tgtaatatgg acaaaataag
    15001 gatatggaat aatttccata tctctaatga gtacataaaa cagaggtatg gaataatttc
    15061 catacctcta ttttatgtat atttattcta atggtaataa atataacaat agtgtaacaa
    15121 aaaaggttcg acatttctgt tgaatttttt acaatatagt gatagactaa ataggtacaa
    15181 attacgtaca aatgaggtga ataaatgaat ataatcttgg catcagcctc tccaagaagg
    15241 aaagaaattc tagagaatac gaatgtaaga tttgatataa ttaaaaatga aattgatgag
    15301 attatattgg aaggtgaaac accaaaacat ttagttatga gattagcttt tgaaaaaagt
    15361 atgtctgtag catctgaaca taatgaagat gttgttattg gagctgatac agttgttgtt
    15421 ttagacaatg ctattttagg aaaaccaaaa gatgagtctt gtgcaagaga tatgttaaaa
    15481 agactatctg gaagagagca tcaagtcata acaggtatta gcttaataaa cttgtgcgaa
    15541 gataaaaagg taattgacta tgtaatcagc aatgtgaaat ttaaaacgtt atcagagcag
    15601 gatattgagg attatctgaa aactaatgag tcatttgaca aagcaggagc ctatggtata
    15661 cagggttatg gagctttgtt agttgaagaa attagaggag attattttaa tatagttggt
    15721 cttccaattt caaggttagg cgacctatta aaaaaatatt ttagtataaa tcttttttat
    15781 ggagtgtgat ttatctgaaa aagtcattta attcaaccat taaggtaaaa gaaatggcac
    15841 cagaggaaag acccagagaa aagatgcttg ccaaaggcgt aaaaagctta tcaaatgctg
    15901 agttgttggc tatactttta agaactggca acaagaataa aaatgctatt gaattagcca
    15961 actatataat aaatagagat attcagggta ttagacattt agaagatatg acaatagagg
    16021 agctgtgtaa tatagatgga attgggttat caaaatcaac ccaaattaaa gcagctttag
    16081 aactaggctc tagagtagct agttttaaac ctataaagta taaaataagg aatccttggg
    16141 atatacagag gtattatatg gatagcctaa ggtatttaaa aaaagaggtt tttaaagcgg
    16201 ttcttttaaa cacaaaaaat gagataatat ctgatgtaga tgtatctata gggactttaa
    16261 gctcgtcatt agtccatcca agagaggttt ttaaagaagc tataagaaga agtgcaagta
    16321 agataatagt gatgcataat cacccttcag gaagtgtaga accatcaaga gaagataaaa
    16381 atatcacatc aagacttatt aaatgtggag aaataattgg aatagaaata atagaccata
    16441 taattattgg agatggatta tactttagct ttaaagaaaa tatgataatt tgactttata
    16501 tgtgataaaa taataggaga atgttttaaa catgattctt aatagtagga aggagtaaaa
    16561 tcatggctaa ggaaaaaaag aaagaaaaaa aaggtttttt tagttttaat aagatgacaa
    16621 aggatatggg aatagattta gggactgcaa atacattagt ttacataaaa ggacaaggta
    16681 ttgttgttag agaaccatca gttgtcgcaa taagagatga tagtaaagag gtattagcag
    16741 taggtgaaga agccaaaaaa atgataggga gaacaccagg aaatatagta gctataagac
    16801 ctatgaaaga tggagtaata gctgattttg acataactca atcaatgatt agctatttta
    16861 tacaaaaagc ggctgataaa aaaggtgttg taagtccaag aatagctata tgtgttcctt
    16921 ttggagttac agaagtagaa aagagagcta tagaagaggc tgcaagacaa gcaggagcta
    16981 aagatgcatt ccttatagaa gagccaatgg cagcggctat aggggctgga ttaaaagttg
    17041 aagaaccaga aggtaatatg gtagttgata ttggtggagg tacttcagaa atagctgtaa
    17101 tatctttagg aggaatcgtt actgctaaat ctataagaat aggtggagac gaatttgacg
    17161 agtctatagt ggcttatgta aaaaaagaat ataatcttat gataggtgaa agaactgctg
    17221 aaaatgtaaa aattaacata ggttctacat ttaaagatga tgaagaaata aatatgcaaa
    17281 taagaggtag agatttaatc tcaggattac ctaaaacaat agaaatatgc tctacagaag
    17341 ttagagaagc attaaaagaa cctgtaagtt caatagtaga tgcaataaaa tctacactag
    17401 aaagaactcc accagaatta gcctcagata taatggaaaa cggaataatg ctaacaggag
    17461 gaggcgcttt gcttagaggt cttgataagc ttattacaca ggaaactggt atgtctgtac
    17521 aaatagcaga aacacctctt gattgtgttg cacttggaac aggtaaatct gttgaagacc
    17581 aagaaatctt tgaaaaagta ttaatgatga atactaagaa ttagaagttg gtgatggcct
    17641 tgagatttga caagaacaaa aatgagaaaa aaaggattaa tgttaaagtg atagcaacgg
    17701 gcgtagttgc tatcacttta attggaattg tgggtatatc aattggtaaa ttttctagtg
    17761 gtagtccagt aaatttagga gtagcttcag atgctattac aagtgtaggt aagggaataa
    17821 atgatggatt ttcttttatc aaaaatggtt ttaaagatgt agcaaatttt aaagataatt
    17881 ctaaaaaggt aaaaaaatta gaagaagaaa atgaaaaact taaaaaaaat atgatagctt
    17941 taaatgccaa actagataaa acagaatcgc tagaagagtt aaaaaagaca ctgaactttg
    18001 tacaagaaga gtataaggcg acaagtattt cagcaagtgt tgttggtaaa aatgatggaa
    18061 actggtatga gagttttgtc ataggtgcag gaaaaaattc aggtgtaaaa aaagaaagta
    18121 tagttatgaa tggtagtgga ttagttggca tagtttatga ggtttccaac aattatagta
    18181 aggcaatttc actattggat tctaaggcat ctgtaagctt taaactagca aaggatgcca
    18241 atgctaaagg tacaattact caaaatacta ctttagataa taaagatagt tataatagta
    18301 aaggatattt gcaaggatat atgtttgact catcatataa tgtaattcaa ggagatataa
    18361 taacaacatc aggtcttgga ttcttcccag atggtatacc aattggcgaa gttgaaaaag
    18421 tggttgatga taaagataag tcattaaaat atgtagttgt aaaaccttat gttgatttta
    18481 agaatataaa tgacgttgta gtcatagaac caagaaatat aggataaagg ggataaaaac
    18541 aatgaaaaaa gttttacttt gtctgttggg gattgtgctt atgactatag aaaatagtat
    18601 aacaaattat atagatattt ttggcataag ctttaattta gttcttatat atgtaaccat
    18661 aatctctctt tatttagatg aattagaagg tgggattata gcagcaataa tagggcttat
    18721 taaagacata accgttggtg gaatttttgg tgtaaatgca ttaattttgt ttattattgc
    18781 atatgcaatt ggatacatga gagatcagct atataaaggt agttatatta ttactttcgt
    18841 tttaattttt ataggcagtt taattgattc aattgtcaat ataggtacat ctagtataat
    18901 ttatcaaagc tacaacatat caacactttt tgtaaaaggt ctactagtag caccaatagt
    18961 gaatagttta attggtttag taatatatcg cttatctaaa agagcagttt taaaacttaa
    19021 agaagattag ttggtgattt catggaagaa aataaacaag ttgatagact aaaaataatt
    19081 aagatagtca tgggcgtgat atttttagct attttagtaa aaataatcta tatgactaca
    19141 tttaaatatg aatattacaa tgagttagct gagaataaaa catataaaaa gttggcaata
    19201 gaagctccaa gaggagaaat aaaagataga tatggaagac tgttggcagg aaataaaaac
    19261 ttatttactg ttcaagtttc aggtaacgat attaataaga aagatgcaaa taagcattca
    19321 agagcaaatg aaatatcatt aaaacttata aatttgctgg agagaaatgg tgaagaatat
    19381 gtagatgagt ttcctatata tgtagaaaat ggaaagtact attatacata tgacagagat
    19441 attagggaat ataaatcaga aaatggtatt ccaaatgatt ataatgctaa ggaaagtttt
    19501 tattacttgg tggacaaact tatatcagct ggaatattat ctcaagaaga taaaagattg
    19561 gatgcgacaa gattacaggc aaaactaaat gaaaatggat attatccacc tattttagtt
    19621 agtaaatgga tgttcacagc agaaagagat aagagagatt ggttggctag ttataagata
    19681 aaagaaacta aactgagtgc caaagaagcg tttgaaaaag taagaaatag tgatgcatta
    19741 gaaattgata aaagtttgag tgatgaagat gcaagaaaaa taatggtcgt aagggatttg
    19801 ataaaatcaa aaggatattc tcaatataat ccagtaacta ttgctaagga tgtgggagaa
    19861 actacgattg ctcaaattga agaaagtgct atggaccttg ttggtgtttc aatagcagta
    19921 gaaccagtaa gatattatcc taatgggtca ttagcttctc atatgttagg atatgtagga
    19981 aaaatgccat ccactcaaat agaatcatac cttcaaaaag gttatgaaac tggagatatg
    20041 gtaggtcttg caggagtaga aaagagtaat gaaagtagat taagaggaac tgatggttat
    20101 aaaatggtta aagttgatgc attgggtaga atcagtaaag aaattgaatc taaaaaacct
    20161 aaatcaggag atactgtgta tcttacacta gataaagatt tacaagaagt ttcagataat
    20221 gcattaaaac agataatcga agttgcttct aaaggaggta cttttaaaag taaatttggg
    20281 gacaaaccaa ttagtgcata tgcaggaaaa gcacagtcag cagctcttat agctattgat
    20341 gttaaaaatg gagaagtatt agcttcatca agttatccta attatgaccc aaataaattt
    20401 gcaaaaggta tttctacaga ggattataaa gcacttcaac caaaaaatcc aaatgactta
    20461 ctagcaggaa gtccactttt aaacttggtt acacaagggg aatttcagcc aggttcttca
    20521 tttaagatgg taacttcaat ggcagcgtta gaaaatggtc tggatccaaa ctttactata
    20581 aatgaccctg gggttataat gttaggtaaa aaatcttttg gtgactatgt atggaaccat
    20641 ggtagaggaa accatggtat gacaaactta tataaagcaa tacaagagtc ttgtaatata
    20701 tatatggcaa caattggtac aggtaaaaca tggccagatg gaaaaagcat tggtatagat
    20761 atgaatgcaa ataaaatact tgagtatgca aaattatttg ggcttgacca aaatacaggg
    20821 cttcaagatg aagtagaaga aagagcaggt aaagttccta gcacagaaga taaattgaaa
    20881 tcaacacaag ctcttctaaa gtcaaattta gaaagagaaa tggcaaatga ttttgttgat
    20941 ataactagag aaaaaaatcc taaagaatat gagaaaagaa tcaatgagat tgtaagctgg
    21001 gcagcagaaa agaaaactcc tggtagagtt gaaacaatga ataggctaaa aaaattggat
    21061 gttaaagaag atagaattga agaagtagca gatttagcag tatttagtta ttttaatttt
    21121 gcaaaatgga gtactgcaga tacattcaac ttagctatag gacaaggtga aaatgcttat
    21181 acaccagctc aaatatcaag atatgtagct gctatagcaa atggaggtaa tttagtagaa
    21241 ttatctgtag ttgatagagc tgtttcaagt gattattcat cagttaaaat taatgaccag
    21301 aagaaagtcg aaaaaatacc ttttaaaaat ccagataact taaaagaatt aacaaaaggt
    21361 atgaaattgg ttgcaagaca aggtacagcg aagagtgctt ttgctgattt tccaatagat
    21421 gtagcagcaa aaactggtac agctgagaaa agtgggaaaa tacctacaga taacgagtat
    21481 gaatatttaa aaagtcatat gtcatcttat aatgtcaacc taaatgatgc gattaaacta
    21541 gctgataaga tgaaagcaga aaaagaaaaa gagctttcat tagctaagga aaaggaaata
    21601 aaaaagaaat tagaaaataa agatttgaaa gacgaagaaa gaaagaaatt agaggaagaa
    21661 ttagaagatg gagtaaaagt tagattagag gacactgata aggtgaattc ttcatatcta
    21721 agaaaagcta taaaagaatt aaatccaaaa ataacagatg accaaataga tagatttaaa
    21781 caagattatg gttcttttac gtggactgtt gcttttgctc cagcagatga cccagaaata
    21841 gctgttgtat gtgtaattcc tcaaggtgat tcaagtgtat tctcactttt accaactaga
    21901 gaagttattg gaacttatat gggattagag ccaactaata gtaaaaatga caacaagacg
    21961 aatgatgtta acaattcttc tgacgaaaat ataaatttcg agtcacaaat taatagatag
    22021 ctcaatctat gtataaactt tagctttata tagaaaaaat aaaagggttt aaagcgtatt
    22081 tgaagaatat aaatgatata tcactctgga ggttgttatg tctttaagag aaatttgtag
    22141 tcaagaacta gtagagttca agggtaataa aagagggata atagtaaata taaaaagaga
    22201 agctccattt gaggaaatac aggaaaaaat aataaacaaa ttggaagcat atgttggctt
    22261 ttttaatggg gctaaaatta gtaaaattaa tagtgattgt ttaactgaca tggagattct
    22321 agaacttaaa gaaggaatta cttctagatt tgatgtggaa tttgttgaag atcaaaaaat
    22381 tgaagaaaat agtaattttc caacaaaata tgtcaatacc ctaagatctg gtgaaaatat
    22441 tgaatttgaa ggtgatgtag ttatattgaa tgatatgaaa cctggttcaa aagtcctttc
    22501 aaaatcaaat acagttgtta tgggagatat aaatgctggt gctaaggttg tagcaggagg
    22561 taatgttttt gtaatgggta aaatagaagg ttttgtacat gcaggtgcag aaggcaatga
    22621 atttgcatat gttgttgctg gaaatcttaa tcctaaaata ttacaaatag cagataatat
    22681 tgcagaagct ccagatgatg aagaaaatta tgaaagtgaa tcagaaatta gcccagaaat
    22741 agcctttgta agtaatggaa gaattgtaat cgaaagctat ttgtcaaagt tagacaaata
    22801 gaaagaaaaa tagggggtag tattatgagc gaagttatag ttataacatc tggtaaaggt
    22861 ggggttggaa aaactactac cgcagcaaac ttaggaactg cgctaagcct agaaaataaa
    22921 aaaacagtag ttgtggatgc agatattgga cttagaaact tagatgtagt aatgggtctt
    22981 gaaaatcgaa tagtttatga catagtagat gtagttgaag gaacttgtag gcttaagcaa
    23041 gctctaataa aagacaaaag atttgataat ctatatttat taccagcagc acaaactaga
    23101 gataaaaatg ctgtctcagt tgagcagatg attgacctgt gtgagaaact aaaagaatct
    23161 ttcgagtata taataataga ttgtccagca ggtattgagc aaggctttaa aaatgcagta
    23221 gctggggcag atagagctat tgtagttact aatccagaaa tatcagcagt aagagatgct
    23281 gatagaataa taggtctatt agaagcaaat gaaataaaag agataagatt agttataaat
    23341 agaattagaa atgatatggt taagcgtgga gacatgatgg ataaacaaga tataatagaa
    23401 atattagcaa tagatttgtt aggtcttgtt cctgatgatg aaagcataat tatatcaaca
    23461 aataaaggag agccagctat acttgattct aagtcacttg ctggtcaagc atacaaaaat
    23521 atcgcaaaaa ggatactaaa tgaagaagtt cctctacttg atcttgaagt tgaagatgga
    23581 ttctttggta gacttaaaaa aatgtttagc atggctaagt agcaaaggag gatacatctg
    23641 tgttagattt atttagagtt ttttctaacg aggctaaaac tagtaaatct gttgctaaag
    23701 agaggttaaa gctagtttta gttcatgata gagtagattg ttcaccacaa cttttagaga
    23761 tgataaaaac agacatttta aaagtaatag ctaattatgc agaaatagaa gatgatggtc
    23821 ttgaaataaa aatgtctaaa tgcagaggtg agcatgatga caaacctgta tctgcattag
    23881 ttgctaatat accactaaag aatataaaag atagatgtat gtaattagtg cttaaaaggg
    23941 ggatatcctt attggtatat gcccctttta tttaattata tgtttaaaga tagaaagacg
    24001 gtgattcttt gaaaaaaatc aatattaatt ataagtcgac aattaagttg ataaaacagt
    24061 tagactggaa acttatagtt acagtattag ctatctttat ttttgggctt gttatactta
    24121 gtagtgcaac acatgctaat tcaacaggtt catataatca attgattaaa caaggattag
    24181 cttttgtact tgggatagga atgattatag tcatattatt ttttgactat aaccttctag
    24241 gcaggtatta taaagcgctt tatataataa gtcttatttt gttagccata gttttattac
    24301 ctggaattgg gacagtaaaa ggtggagcta ggtcgtggat taacttagga ccacttgact
    24361 tgcagacatc tgaaatagtc aaattaacat ttgtactaag ttatgcaaag atacttgaat
    24421 ccaaaaagga taaattaaat acgttaaagg aagttatgcc tgttgtagtt tattctcttc
    24481 catttatcgg attattgatt gcacaaccag acttgggaac aggaattgta ttttgttgta
    24541 tgatatttgc aatgctgttc acagcaggtc ttagctcaaa gttgataaaa agaggaataa
    24601 tcatactatt agtgtcaatg ccactgatgt atttaatgat ggcagaccat caaaaggtaa
    24661 gaatagaagc ttttctaaat ccagaagatg taactctaaa aggaaactat caagttatgc
    24721 aatctctgat tgcaattggt tctggaggcg taactggtaa aggactttac aatggaagtc
    24781 aaaatcaaga agatttttta cctgtacaag atagtgattt tatatttgct gttgtaggtg
    24841 aagaacttgg tgtaatagga atggcagtgt tgataatctt atttatgata ttcctattaa
    24901 ggcttttagc aattgcaaga gatgcaaaag atttttatgg aaccctcata gtggttggtg
    24961 ttatgggaat gtttggttac cagataatcc aaaatatagg tatgacggtt gcgttaatac
    25021 cagtaactgg agtaacatta ccttttgtaa gttatggagg aagttcgtta ttgacatctt
    25081 tagcaaactt aggattagta ctaaatgtgt gtatgagaag aaagaaaata aatttctaaa
    25141 tttaaataca tctaaacata aattgaggag attagatata tgaatatagc attagtagca
    25201 catgaccaaa tgaaaaacac tatggtcgga ttttgtatag gttatgaatc aattttaaaa
    25261 aagtatggac tatatgcaac tggaactaca ggaaaaagaa taatggatga aactgaatta
    25321 aatataaata gattagcatc tggtccttta ggtggagacc aacaaatagg ttctttaata
    25381 gtaacacaag aaatagattt agttattttt ttaagagacc cattgacatc tcaggctcat
    25441 gaaacagata tacaagcgtt aataagactt tgtgatgttt atcatgttcc aatagctaca
    25501 aatctagctt cagcagaaat atttataaaa gctcttgata gaggagaact atcatggaga
    25561 gaagttagaa aaagcaaaag tcaacgtatt taaaaaatat taatattaca gtctaaagaa
    25621 acagatatat aagtgtttaa tgccctagtc tttctgttgg aataaatcta gtacctgtag
    25681 aaatcttatg aaagatttaa atgatgaaga gctaaatttg agattaaata attaggaaat
    25741 agattataga tagagtagta gaatttaaac tattactcta ttttaataat atgaaataga
    25801 atcaagtcta ttattatata tatttaaact tataggtaat actatttcaa ccaagtaaat
    25861 gttgttatga caacattaat aaaacaaaaa tttattttta aaatttgata aatagctgta
    25921 tattagtatt tatagattaa atattaatat acagctattt attattttta agtctattgt
    25981 agaatataaa ccttgcaata tagagtacta tgtattatta tagtaaatat aagtactata
    26041 cattatagag tagtaggtga aaaggcggtg gtacataatg cagttaaata aagaagtgtt
    26101 aaaaggttat atcgatatat taattgtttc tatattagaa aagaaagatt gttatggata
    26161 tgaaattgca aagcaagtaa gagaaagaag tgagtttgaa ttaaaagaag gaacaatgta
    26221 tcttgcactt aaaagaatgg aatctaaaaa tttaattaaa tcttattata gcaatgaaca
    26281 aagttcaggt ggaagaagaa aatattataa tttaactaat gaaggtaaag attttttaga
    26341 aataaagaaa caagaatgga gatttattaa aaaggttatg aatcaatttt tgggagaggt
    26401 ggattaattt atgaaactaa tagatgaata tttagataaa ctatataaaa aatgtgacaa
    26461 taaaagtact atagaattaa aacaggaaat gagatgccat cttatagaat ctgcaaatga
    26521 atttaaatta gaaggattag atgaagaaga agcatgtaaa aaagcaattg aaagatttga
    26581 tgatggtgat gaaatgcagt atgaattatg taatataata aaagaactat ctttatcatt
    26641 ggataggcat aaatcaattg taatggggtt taaaaaagta ttaggttaca taagtataat
    26701 tgcattttta atttctggat ttatgtggta ttacaataat agtttacaac ataatatgta
    26761 taatttagga aaagaacttg atggagaaat aaagcaattg gcagaaagac atgatatgac
    26821 aaagattggt gaatataagt tagaacttga aaaaatactt gataaagata agtatagtaa
    26881 agtcaaagct cttagactat atgtaataga tatgaaagat ggaaacacta atttaagttc
    26941 ttctggattg aatgctaata tggtatatga gagggaagca gattataata acataagtaa
    27001 ttttatacag catttaggat ataatggaaa agatttttta gacaagaatg ggaatatagt
    27061 aaatccagat attttcttag aatatttctt ttattttgaa tcggagatgt taattccagt
    27121 agcatttgca tttggattat tatgtataat agcgtatttt atattaagat ttaaaatttc
    27181 tttaataaaa aataataatt aaataattca ccaaataatc ttaatttgta gagtaaataa
    27241 gatgtttcaa aataaaattt gaatcatctt attttttatt gtttgttata ctcttttctt
    27301 ttatatttct ccatagtgga ttgtatctat acataatcac atattttgat ataattatac
    27361 tttccaaaaa atgtattgaa tatacaaatt ataatctgta taatgttgtt aggggagaat
    27421 gtatgaagtg tatacaattt taattttaaa tgtattattg acaattctcc tcactctcgc
    27481 cattaccaat aattggaaaa ttgacatacc tgttacagac caacattttt gaatatatta
    27541 attattatca ttttattgtg atgatatcaa aaatgcaggt ttgtgagttg gattgtatac
    27601 gaactatata ggaaatatag aacagatttt ccaagaaatc taaatattct atgaccacgt
    27661 gaattgctat gcatacatgt tgataatcca tattttaaat ctaaaatgaa cttaattaat
    27721 ggtagggaat tgaaagtaaa atatattata aaggagaatg ctaatatgaa aaaaatcagg
    27781 aaattagcta ctaaactatt gattgcaatg atggttttaa cctcatgtat gagtgtaaca
    27841 gtttatggta tgacagctaa agatgttact gcaaagacac cgaaaagtta tgtagctgga
    27901 accaacagta tatatggtcc aaagttatca caagctcaac ttaattcggt agcacaagca
    27961 acagcagact ttatgaacaa gaaaattaca aagaacatga ccacagatgc taagattagg
    28021 gttgcttata atcacattaa aaacaacaca acatatgtta actggaacgc agcagaggga
    28081 gccaatactg cttatacatt ggttactaaa aaaggtgctt gttctggaat gtgtaggagc
    28141 ttgaaagctt tatgtgatgc tatgggaatt gaatgttatt atgtacatag tacaacaaat
    28201 gaccatcagt ggaatcttat tcgttttgag gatggacgtt tgtatcatgt agatattgat
    28261 gccaacaaat cagcaggagc agacatcata tacaagagtc ttactcaccc attaccattt
    28321 gacaagaaag catatccagc tgttggcgaa gtagtaaaga cacagaatcc aacgccaact
    28381 gttaagaaac ctacgccagc taagccttct gcgccagttg gacctgggaa tcacactatg
    28441 atagcagatc aacaaggggc aatacttgat gaagtttatg gaaataatct acgtccaaaa
    28501 attgaaatgt atgagggagt tccgtgtagt ttaatcaact catcaataaa tacaattaag
    28561 tttacaagca aaaattcaaa tttagaccca tcaaagcata cagttactgt aatagggttt
    28621 atgaatacac catatggtat ggttgcatta gctccagatg gtgtacctga tgagtatcct
    28681 tttgaatatg ataagccatt ttctttagat tttactggta agaacctttc tgcagcagaa
    28741 ttatcaaaaa agtgtaaaga aaataatgta attattaaag tggaaattgg tgaaacggca
    28801 tctaattctg gaagagggat ggtagcatac gctagatatg tgaattaata caaatatact
    28861 agtgtacaaa tagaacatat tataaataat gcaccctcgc acaaagggtg catttatcat
    28921 tatatcagtc attttagaaa ataaaaaagg agttgaactt aaaattagat tagaaaaatt
    28981 aattttgaga caactccatt ttaatttatt tttaaatata aaatcattta ttttaaataa
    29041 tattatgttt gattaatggc tatttaacta attttgcaaa ttctgcacca aatgccatac
    29101 actcttttaa tgtctcttca ctagggaaga attttttctt aagagattct acaggcattt
    29161 tgaatgacat agatttaagt aaagtctcag ccatagttat accttcacca ctccaaccaa
    29221 atgaaccaaa tactcctgct atcttgcctt ggttagccat tgggtctata acagagaata
    29281 aatcccacat aggtttaacc atagttttgt ttatagttgg agaacctagt aatattactt
    29341 ttgacactac taatgtatcg tgcatttctg ttaatgtcat atcttctaaa tcatataatt
    29401 cagcctttgc accttcttta tcaagtcctt cttttatctt tttagccatc tctaaagtat
    29461 tactataagc tgataagtag aatatagaaa cttgattttg attagttgta ttaacagctt
    29521 ctgtagacca ctcaacatat cttttaacag cagccattgg gtctttagta agcattggac
    29581 catgagaagt caatatagta tcaaattcta tgtttagtcc taccacctta tctacagcac
    29641 ttaaaacatg tttagcaaat ggcttaacta tacaatcata gtagtgttta gctgatttta
    29701 gataatcttc actattcact acctctgcat caacacttgc aaagtgacaa ccaaatgcat
    29761 cacaagttaa aagagtcttg tcctcctcaa tgtaagtaaa cattgtatca acccaatgta
    29821 agaatggagc agttataaat ctaagatttc ttttaccgat atttaaaatt tcaccatctt
    29881 ttattacatg acagttaaaa ggtctattta tttgaccatc taagtatagt ttagcagctt
    29941 tagtacaata aacttctata ttaggattta tatctaataa gtattttaaa ctacctgcat
    30001 ggtctggttc tgtgtgatga attacaacat agtcaatttt agctatatca gtaacctctg
    30061 ataaattact taaaaactca tctttaaaat ttgcctttac agtgtcaaat aaaaccgttt
    30121 tttcgtcctt gattaaataa gagttgtaag ttgtcccaaa ctcagtttcc attattatgt
    30181 cgaatacttt taaaccttca tctactactc cagtaaagta gatatctttt tttacttcaa
    30241 aaactttact catttttaac cctcctaaaa tttatcatta tataagtaac ataccccatt
    30301 ttgaggtgaa tgaaacaaaa gatgtcatga taagtcatgt aaa
//
LOCUS       DAELLV010000023        29862 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.023, whole genome shotgun sequence.
ACCESSION   DAELLV010000023 DAELLV010000000
VERSION     DAELLV010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29862
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.023"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     regulatory      225..310
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            472..993
                     /locus_tag="KPD58_000883"
     CDS             472..993
                     /locus_tag="KPD58_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG0264667.1"
                     /translation="MKLKKNKKGFTLVELLVVIAIIGILAVVAVPALFSNINKAKVAS
                     VESDYSSVKSAALSYYSDTNKIPVTPDGQTGLSVLETYMESLPDKADIGGKYKLIKVG
                     NKLVLQIGTNTEGVTLTEAQSAKLLSDIGENKIYTSTTTNSLGNPLTSNTKIDNNVLY
                     IVLIDNTVMDTTK"
     gene            1144..2820
                     /locus_tag="KPD58_000884"
     CDS             1144..2820
                     /locus_tag="KPD58_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HBG0264668.1"
                     /translation="MAKKVRIGDKLVEKGYITEEQLKWALSEQKNSGKRLGEFLVQEG
                     LIDSNLLISVLKELLDIESIFLEGTEIDTLATKMVPENICKRYTVFPFKIDGNKICLA
                     MSDPQDREAVQDVRRMSGKDVEIFISSTEDINKAIGHAYAHSEINKAMTEYNKNRTGG
                     VRETVILEEDVNAAPIVRLVNNILENAVRMEASDIHIEQSENYMRVRFRIDGMLREYM
                     RMNSAPYKAVISRIKIMSDINISEKRIPQDGRIYLKVDNKPIDFRVSTMPTNRDEKIA
                     MRVLDKSNFMVSKEVLGIDEHGSKIYDELINTPYGLILVVGPTGSGKTTTLYSMLNQL
                     NTENRNLLTIEDPIEYELPGVNQSQINEKAGLTFASGLRAFMRQDPDIIMVGEIRDTE
                     TAEIAIRASLTGHLVLSTLHANTAVGAISRLLDMDVESFLITSSVLGVISQRLTRKIC
                     EHCKVSYEADIGEKKALGIDVNESVTIYRGKGCERCNNTGYKGRLGIFEMLEITPEIK
                     ELIDSSANQREILKMARKQGMVSLKEDIVKKVLNGKTTVEEMIRIILMTD"
     gene            2832..4040
                     /locus_tag="KPD58_000885"
     CDS             2832..4040
                     /locus_tag="KPD58_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBG0264669.1"
                     /translation="MKSFKYTVIKEDGKKQTDVIEANDFNEARNLLRKRNLRVIEIKE
                     SKNKNIRLSSIKRKKDLGADQISHFCRQFAIIVSSGINSISGLETLARRSTNITLREE
                     INRIVAEIKVGSTIADSMLSSKSKFPKLLGAMVATGEATGKLDEVLKSMASFYASEHR
                     VKQKLRNAATYPLIVLISSFIMIFIFTTFMVPKMINSITTVGATLPLITRIVMGFGMF
                     MKKFWIIVLLVIILFIYQFKKYLKTSVGRAHKDRVINKIPVLGKGINCMVATRFSRAL
                     YLFVSTGYPLVQGLDYIIDSVNNTMAEKILASAKDGITRGEGLAENLERYTYFDSVLV
                     QMIAIGEQTGELENISRQMAEFYEYESEIYLNRMASMIEPVLIIAVGIIVAILVVSIF
                     MPMLSIYDAM"
     gene            4055..5767
                     /locus_tag="KPD58_000886"
     CDS             4055..5767
                     /locus_tag="KPD58_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HBG0264670.1"
                     /translation="MNKVYVSYYGDSISIVEGRYKHDKDKFYVKNFNVFSIEDIAQDF
                     SKEKYALLRYALENVKLKSRHVVFCLNTRDVILKPQKLPNMDKKDLDGFMKLEMDEMM
                     ALESDEYVFSYEVSSETPDNSDGDESSLNLIIGAIQKEEVNAIVEIMHEFDLVLDRID
                     TLSTAYLRMLKNLDYNDIMVTNISDNSTIINIYKKDTLFISDNIPIKITAKDMDMQLL
                     NVVEESKGLMNYYSSRNFGKVTDTILILGKRYSNKLIYETFSSAFSSYVSQGLTEIMD
                     ISDMITGDIDEDDINSIVELLGSMIEPKGKRDFENINFLPMDILRKQIKAKQMKNILK
                     IAPIVILILAIPYLCLIFSNNLANKELENVNGEIRNIESAYYQIGNIEKMIQSKSEEI
                     KVYDMLIGKKVKWENLLDDIDKNTPSKVELTSLSTTYQNLGTNNKADNSKSNKTTTDK
                     SSNNETKSSTKSDSNGDKTDNDLNEDKTTNVSEALTGNSETNSNNTDKDDKTPLYDKI
                     PNVINIEGNAKDSSYVGQFLYKLKGLYYFKDVKLHSIKSNAEDSNYTFSMTLYLKEGV
                     LTNE"
     gene            5760..6668
                     /gene="pilO"
                     /locus_tag="KPD58_000887"
     CDS             5760..6668
                     /gene="pilO"
                     /locus_tag="KPD58_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HBG0264671.1"
                     /translation="MNKDIFKKDIKDIFKMEVSLKGILIKVIILLAILSYGYFGLYPQ
                     YQKYIATKNSVKQATEKLNIYKEKTALMPKYEKQLEEVESELKEKSKNLLYDMQDGLF
                     LIGLDKKMKAYNIDLTSYDVGNVKEYEHFYGISTTISVRGDYRNVKKLMAYLEEQKNV
                     TQILDYTMTSYIEPEKEEEETTNNKDLIKNVNINKDTKIYWVEGENKWHLYKDCPLIK
                     EGEIIKNDKLLDRSTNYLCNTCVLRAATEKEAKKEVVAPKAEGVVDATFKFIMYTKEN
                     TTMILDTDNPSSWRPGKYNPFKSSAK"
     gene            6695..7261
                     /locus_tag="KPD58_000888"
     CDS             6695..7261
                     /locus_tag="KPD58_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG0264672.1"
                     /translation="MSLYKNNEKGLTLLEVIIAVFILTIVLSISYKVFNGITSAVKKQ
                     QIITDAQVNINLINKYLNRDLENCKELTKTGSGNNYEYNIEMPDNVVKYEVSIETKKN
                     TEVYSVTRIQNNTIDTENEVREEIIYNQPLVQNNKEMKETPFKIEKQTGKSIYTVSIY
                     YNESVQESHKNSNLNNKTYTFDVMSRIG"
     gene            7298..7825
                     /locus_tag="KPD58_000889"
     CDS             7298..7825
                     /locus_tag="KPD58_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG0264673.1"
                     /translation="MSKKNSERGFSLIEVLVAMAIMGIVLFAFFNIINTNNKANTKND
                     TDITSLNYVQSEIENLREKIKSGEFDFDSLDKLEDGTVVYEKLIDKSKKVVYDKVLSE
                     GDVSLYDTPYEKITTIKDEDGNLIDKGNITNKIKTIVEDKSGQIYKIAVTGKSMNDYS
                     SKKEVKIVTEIFKDK"
     gene            7846..9384
                     /locus_tag="KPD58_000890"
     CDS             7846..9384
                     /locus_tag="KPD58_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264674.1"
                     /translation="MRKWNKFKSERGAALVLVLVVVALLSIVGLIFSNQIANRIKSTK
                     TTNEGIQAKYLAETCVENSIDKAYEKLYDELEKMDNEFKSENQEKSISRSKLRNISDE
                     DFNNQDEENIEAERLGYMNNINFYLNKASSDLEKASMELKKLYDLDMLDYRDIEYVDA
                     NIISHRDSILEICKNYTSGDISKINEYILKEDIDSTTLIEAKLVNNDILLKKFLEENK
                     IENEHLNSAFSHTYKALDNISLAMQNMIEYRHTFHIDEPKVEVSNGIPDSQQYYELIQ
                     NPIINSMEYSWNSKWDTLENLLEILPNQTQGFNSLRVHLRNNVRKFEKLSDNISSGKK
                     NTAKNFLKYKELLYEISDQCNQLKSMSYEKIPVKYDNMALITTFDYIQNELLAEIKCR
                     LKELKPQEIDKTEGITIKIPFYKADYDMTKEGWPKLKENGSGAELSLMVTGDKNGIKE
                     VEVTDGKKNIIGLGVEENSNSKYKVDAIVNFNLNIDTNVVGNYDIKDKILINHDISSY
                     KKVN"
     gene            9480..10562
                     /locus_tag="KPD58_000891"
     CDS             9480..10562
                     /locus_tag="KPD58_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HBG0264675.1"
                     /translation="MYIYDLLEQGIRLNASDIHITVGTNPVARVKGGFVKLSEQILTS
                     EVTMQMAKDIAGESMFKVIEEHGEADFSASLKTGERFRVNAYRQKGNYAIAIRTITAE
                     IPTFEKLGLPESIKSFTEKHKGLVLVTGPTGSGKSTTLASMINIINEKQQKHIITLED
                     PIEYVHHHKQSLVNQREVGTDTESFHSALRAILRQDPDVILIGEMRDPETVSIALTAA
                     ETGHLVFSTLHTVGAAKTIDRIVDMFQPSQQQQIKTQLSTVCEGVVSQQLLPTADGKG
                     RIAAIELMFATPAIKNLIREGKTYQIPNMIQTGVKVGMKTMDQDLMELYKNGKITKDM
                     ALSRCTDQEFMTRMIGGVNYNGYYNR"
     gene            10543..11325
                     /locus_tag="KPD58_000892"
     CDS             10543..11325
                     /locus_tag="KPD58_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBG0264676.1"
                     /translation="MDIIIVNLYVFIVGIVFGSFFNVCIFRIPEGISISRPPSHCMSC
                     NTRLKPIDLIPVLSYLMSGRKCRYCYEKISSRYAIVELITGLLFLTVFMLYGISTSTI
                     YYLVLVSLLIIITFIDIDHFIIPDKILVFGAIFSMIFNLLFKEIPVKDSILGVFICGG
                     SVWIIVLLIEFVIKKECMGGGDIKLFAMLGLYMGVKNGLLTALLSVYVGAVYGICVII
                     ISRIKGKEYNSVIPYGPFISIGALITILCGKKLLELYFSMVI"
     gene            11427..12077
                     /locus_tag="KPD58_000893"
     CDS             11427..12077
                     /locus_tag="KPD58_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBG0264677.1"
                     /translation="MEYIIIISLFFAHLINKSLCVFCDREKKNLYNTFFIYIITVVLS
                     VLIYLKFGISVECVQYMVLIPFLIIISIVDYYTIYVYDITIVSGIIVQGIIFLAFFNI
                     AQFDYINHIFGLLIGFIIPYIIAKLTKGLGYGDVGLYSLCCFALGNNYGFYVIVLSFI
                     LAFLFFIILYITKVFKDVDIRKQIPFTPFISLSTILIIFTEYNIFNFYYNLLYKFI"
     gene            12091..12651
                     /locus_tag="KPD58_000894"
     CDS             12091..12651
                     /locus_tag="KPD58_000894"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBG0264678.1"
                     /translation="MYVVVGLGNPGKKYEKTRHNVGFDVIDILAKEYNISVTKIKHKA
                     LIGEGRVGTEKVLLVKPQTYMNLSGETLIDIYKYYKVDLSNIVVVYDDIDLEVGKIRI
                     RKKGSGGTHNGMKSITNCLGSNDFPRVRVGVSKPEAGQDLADFVLSRFRKEESDNINE
                     ALEKAADAIDSIIRENIDMSMNKYNG"
     gene            12668..16054
                     /gene="mfd"
                     /locus_tag="KPD58_000895"
     CDS             12668..16054
                     /gene="mfd"
                     /locus_tag="KPD58_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBG0264679.1"
                     /translation="MNDVFLYPLQNSKEYKDIINCIKNTKGSLLVNGLLPVQKPHISY
                     SIFNDLSRQMIFITSSDLEAKKVYEDLSFYMEDKVEYLGFQDIYFYHLDAKDRNEEAK
                     KLKVLLKLANKEKIILVTSIEAVLRKYIPKQVLLDSVSHYKVGDSLDLEKLTEKLVSL
                     GYERVSKIEGFGQFSIRGGIIDVFSLEYTNPIRMELFDDEIDSIRTFDVYSQKSIDKL
                     QQFSITPSREFIYPEKTTDALVKLKKETTKNTDEGTFQNIDYISSKTYFEGVENYIDY
                     IYPEENKSIFTYLADDAIVFINDITRLKERCENYINEFRENYKLNLERGLAIKNQGKL
                     LYHYTDLEYLVKDKSVVLNSLLPKSINNFSIKSIINFESREVPTFNGKVDLLVEELNR
                     LKYNGYKIILATNTLERANKLGKDLLDKGLETTISKDRDIEIKSSQVIIVPAHINSGF
                     QYKSIKFVVITDNEMIGVYKRASKTSNKKVKKGKKIESFLDLSVGDYVVHENSGVGRY
                     TGIEQITVNAIKKDYMKIVYQGGDNLYVPIDQMDKVQKYIGAEVEKVKLNKLGTNEWT
                     KAKAKVKKEIEDMTKDLIELYAKREKIQGYKFSKDTPWQAEFESLFPYQETEDQLKAI
                     EETKKDMESSKVMDRLVCGDVGYGKTEVAIRSIFKACMDQKQVAVLVPTTILAQQHYN
                     TFKERFENYPLRVEVLSRFKTPKQQKQIIEDAKKGLVDILIGTHRIISKDINLPNLGL
                     VVIDEEQRFGVKHKEALKKIKSTVDVLTLSATPIPRTLHMSLSGIRDMSVIEEPPQER
                     HPVITYVTESKESVIQDEIERELSRGGQVFFVYNRVEHIEEMASMIQKLVPDARVAVA
                     HGRMTSKSLENIILGFLNKDYDVLVCTTIIETGMDISNANTMIIYDADKMGLAQLYQL
                     RGRVGRSSRQGYAYLLYEKDKTLSEIAEKRLKAIREFTEFGSGFKIAMRDLEIRGAGN
                     ILGSQQHGHMAVIGYDLYVKMLNDAIKKVKGEPIVEEIDVEIDLSVNAYIPDNYIKDE
                     LIKIEMYKKIASIENKEDMLDIQEELEDRFSDIPKPVQTLLTIAYIKSLCKILKIEKI
                     RQLKDEILLVPITKYRTKQKIGYNIVTELEELLEKMCKVK"
     gene            16085..17080
                     /locus_tag="KPD58_000896"
     CDS             16085..17080
                     /locus_tag="KPD58_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG0264680.1"
                     /translation="MKKVITLVIAMILVVSVTACSSSKGETVATVEGTKISSDEFKKT
                     IALYKDSMEQTYGKDIWDKEVEKGVKYKDKFKDLILDQLITTEVIYSQAKKDNLLPKK
                     EDVEKSFKELKDAMGKDEKYKEQLKKLGIDDEFLRDQQEKDLAMQNYQSNFAKKTKIS
                     DEEMKKYYDTHKDEFKKDEVEASHILLKTVDDNNKPLSDKEKAEAKKKAEEALKEVKS
                     GEDFAKVAKKYSQDTSASDGGKLGFFSRGQMVAEFEDAAFSMKKGEVSDLVETQYGYH
                     IIKVTDRINEQTSFEDAKETIKDQLLKNKYQEQIEKLTKEAKVEKDEKVINKITI"
     gene            17204..17743
                     /gene="spoVT"
                     /locus_tag="KPD58_000897"
     CDS             17204..17743
                     /gene="spoVT"
                     /locus_tag="KPD58_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HBG0264681.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRKTLRIREGDPLEIFTAKDGEV
                     ILKKYSPIGELNEFSQEYAETLAEVLGYGVLITDLDSIIAVSKLPKKDYKEKSISDEL
                     EAIISERTVKYSKDKNILPLFKEDQKEYTSQVIMPIISASGDCIGSIVVVSKDKDTIA
                     ESEEKSLKIAANFLGKQVQ"
     gene            17863..19476
                     /locus_tag="KPD58_000898"
     CDS             17863..19476
                     /locus_tag="KPD58_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBG0264682.1"
                     /translation="MNNNKNKDSFLKGALILGLAGVIVKIMGGFFRIPLGNMIGSEGM
                     GYYQAAYPVYTLFLTLATAGFPTAVAKLVSEKVAIGNFKGANKIFKVSHTVLFITGII
                     SFCILFFGADYIVTNVMKNPGALYSMKAIAPALLFVPAMSAYRGYFQGRQDMTKIAVS
                     QVAEQFFRVVLGLTLAYLLMKSLGQTFGAAGAISGATIGSIASMLYLVFAYMLGRKER
                     RAEIDASQRFKDERVSYIFKKLLTVAIPITIGASVMPLVNMIDNVIVIRRLMEAGFTY
                     KVANSMFGQLTGMAMATINLPAVITTAMSMSLVPAISKAYALGNKSKARKDTKSAVKV
                     TLLIVLPCAFGMASLAIPIMGLLFPHEPSSVGTILFTLTPCVLFLGLIQTLTGIIQGM
                     GKPIVPVIALCVGMLCKIVISYTLTGIPDINVLGSAFGTVTAYFVAAMINLLYVKKHM
                     NVNFSKKEFIIKPFITVMTMFIMVKLSYGALVGFLGNSISTIIAICVGGIVYVVVILG
                     IGGIKKEEILTMPKGDKLYKLLKKVKLIR"
     gene            19583..21031
                     /gene="mazG"
                     /locus_tag="KPD58_000899"
     CDS             19583..21031
                     /gene="mazG"
                     /locus_tag="KPD58_000899"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HBG0264683.1"
                     /translation="MGKISIVGLGPGDYSLISQGVLESLINSPKVFFRTEKHPIIDKL
                     KETIQYTSLDYFYEKEENFDNVYLKISEFIIEESAKGDLVYAVPGHPRVAEKTVSIIE
                     RLANEKHIAVEILPSMSFVDAMFNYLAIDPSEGFKLVDAFEIENSYIDLDTSMIITQV
                     YDKFIASNVKLKLMEHYDYNQEVCIVNGAGVKDLESKKFIKLCELDRNENFFDYLTSL
                     YIPKSSKKMYNTVHDLEIIVNTLRSPSGCEWDKKQTHQSLKNSVIEEAYELCNAIDNN
                     DIDEMVEELGDVLLQVIFHCQIGNEEGYFDLKEVVNGICKKLIHRHPHIFSGAKLDMS
                     KFEKTWEELKKEEKGESTITDGLRRIPKHLPALMKASKVQHKAALVGFDWDNVEDVFK
                     KIEEEYQELLDEHRVGNIKYIKEELGDLLFSIVNLARFLDIDSGEALNCTTEKFINRF
                     DFIEKSAMNLNKKLEDMTLEEMDEFWNQAKKQ"
     gene            21120..21398
                     /locus_tag="KPD58_000900"
     CDS             21120..21398
                     /locus_tag="KPD58_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003153447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBG0264684.1"
                     /translation="MNKAELVSKMAEKSGLTKKEAEAALNAFMSSVQDALVNNEKVQL
                     VGFGTFETRERAARQGRNPRDPEQVIDIPASKAPVFKAGKGLKDIING"
     gene            21470..21712
                     /locus_tag="KPD58_000901"
     CDS             21470..21712
                     /locus_tag="KPD58_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HBG0264685.1"
                     /translation="MRLDKFLKVSRIIKRRTVAKDACDKGIVTINGKVAKSSSEVNIG
                     DTLEIQFGEKKMKFKINEVKEHVLKNDAKEMYEILE"
     gene            21774..22028
                     /locus_tag="KPD58_000902"
     CDS             21774..22028
                     /locus_tag="KPD58_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HBG0264686.1"
                     /translation="MEQNITLKDRSKLVISGVEHIYSFNDKRVELKTSVGEMVIEGEN
                     LDMSKLSIDENIISIDGTINSMVYAKPKKPQESFLKKVFR"
     gene            22029..22562
                     /locus_tag="KPD58_000903"
     CDS             22029..22562
                     /locus_tag="KPD58_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264687.1"
                     /translation="MIPFTQDVSIFYATIYGGILIGVLFDFYRGLRGNFKFINYFAII
                     FDVLFWFLATVIIFVTINLTEFFDLRYYHFVALFIGFILYYNTISKIVLSIINKIIRF
                     VRNSFKKVTHYIVSFLNNLYYVIIYSLHLLFDIIFYIPNIFIATRKSIKRKSNKKLKN
                     KKKSKPKKKKNKTKKRV"
     gene            22568..22852
                     /locus_tag="KPD58_000904"
     CDS             22568..22852
                     /locus_tag="KPD58_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HBG0264688.1"
                     /translation="MNLRKKFSGQIIVISLFLGISIFSMMTGFVFEYTKAKEYKKEIA
                     SLNKQLKKTEIQINSLKKDEKSYEGDLEDIARKRLNMVKPNETVYVDINR"
     gene            22965..23885
                     /locus_tag="KPD58_000905"
     CDS             22965..23885
                     /locus_tag="KPD58_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HBG0264689.1"
                     /translation="MKVGAIDIGTNSMRLLIADYRDGNFINRKKFVNTTRIGYGVDSK
                     GYIKEDAIKRNIKALKEFSDKCNDEKCEKIYCMGTSALRDSKNGDYFVKLAKEETGID
                     VDIVTGIEESNLGFKGVLEGIQGDDTIIVIDIGGGSTEFIVGDSEGIKFNKSENVGAL
                     RMTERFLKHDLIEDEEFEAMSSFIYGEIDKTLNHIKDRGLKTLIGIGGTITSLSAMNQ
                     ELEIYSMEKIHNSKIHKKEIEEILQKLIKMTLSDKKNLKGLQEKRADIITAGVKILNI
                     IMEKLEIEEITVSEYDNLEGLICQKSKKMY"
     gene            23975..26347
                     /gene="spoIIE"
                     /locus_tag="KPD58_000906"
     CDS             23975..26347
                     /gene="spoIIE"
                     /locus_tag="KPD58_000906"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HBG0264690.1"
                     /translation="MQRSVAVVKNKTVKPNVIKLISGYIDFTTAAFAVMGFLLSRSII
                     VDSVAPLGIAFFICASKIDKYKIPVFISTLLGILLSSNDFGNVAKYAVCLTIFMAMNK
                     KLKLSDSIAKVAFIGAIILLPISMGQAFFSNRYVYGLLIAGVESIIMFISIYVFSYGI
                     GLMANVNNRMSVRTEEAISISLLLTFSIMGIGDISLFGISIRTILSTVLILLTSIVGG
                     ATMGASSGVIIGVASILNNMTSAVYMGIYSFSGLISGAFNKINKYFCILGYILSWTII
                     YLYTSGITSNMMQLRDILLGCLIVLVLPERLFNKIEKLIKSNVASNEIVYDYIMRSKN
                     LTNSRLNSIYKTYDDLADTFDKIREKDKVLDQRDIANVIDMIHNDECKSCSMRRMCWE
                     SRFNHTYTMIYNILEKIEEKGELSLNDIPKNFRKECMKPESIVKISNHYYKMFVLDYD
                     WSVKFSESRKLIANQIRSISKSIKSLSQDLEGDIMLDIEKEKNIYEQLERYDITVDKV
                     SYLTKSNSEFEISIEKKTCHDGCMCEDKIVNIISDLVGENMSVRKIGCHCLGGKCKAT
                     FVKSQKYKAVTEVSAMSRDGHILCGDNYTYMEINDGKYMMAISDGMGKGKKAYEESSA
                     TIDILEKMIDAKIKDEIVIDTINNMLLLKSSEEMFSTLDLGILDLKRGCLETIKMGAC
                     STYIKREDGEVDLISSSSLPVGILSDVKIDRKNVKVKEGDYVIMVSDGIVDAGRNNNL
                     GDNWLIYFLKNIETTNPKEISNLILDRALELQALQIEDDMTVLVTKICTK"
     gene            26698..28590
                     /gene="pepF"
                     /locus_tag="KPD58_000907"
     CDS             26698..28590
                     /gene="pepF"
                     /locus_tag="KPD58_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBG0264691.1"
                     /translation="MNRKLCKAIIPLFISIFCMLIISIKADQGYQKNNDRAITVMNNN
                     DTEDDAEKKQEIDYEKTCWNLQSLFKSDDQWKKELKSFDKDTKELKNYIGKVTKSPTH
                     LSFALNIKEKLDIRLNKLSAYVKLKQDTNKNSYKYLDMNDSMSKSYGNYMGICSDLEL
                     EILKLSNKDYKKIISNKNINRKYGAYLRDIRRNKVHYLDDKSEDILSKVSSISSLPSQ
                     VYELFRNMDRKTNLTPAQYASELESSDRENRKKAYQDELITYNDNINTIAGLITGQVK
                     KNIFYSQERGYESSLEMYLESDNVDEKVYNNLIRTVNSNMDSLHKYIDLRKKVLKLDK
                     VHSYDMFVPMVKAVDNNINYEKAQSIVYSALSPLGKEYNDVVYKAFNEKWIDVYSHEN
                     KVSGGYCLSVYENHPYILLNYNNSLGSVSTLSHELGHAVYEYLSSKNQNYFNSNPSIF
                     THEVASTTNEALLYEMLIKEAKGNDEKAYYITQYLDLIKDTLYTQTMYAEFEKTIHEL
                     VEKGKSVNALVLNDVWGQLLKKYYGQSYELDQLSKVGWARIPHFYNSFYVYKYATGCS
                     AGVSFAQDILKNGSDNYMSFLKKGSSDYPINLLKSSGINLTNTKPIENTIKKFDSLVA
                     ELEKLL"
     gene            28704..29669
                     /gene="galU"
                     /locus_tag="KPD58_000908"
     CDS             28704..29669
                     /gene="galU"
                     /locus_tag="KPD58_000908"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBG0264692.1"
                     /translation="MQVKVKKAVIPAAGLGTRFLPATKAQPKEMLPIVDKPTLQYIIE
                     EAVASGIEEILIITGRNKKSIEDHFDKSVELELDLEKKGKKELLEVVQNISNMINIHY
                     IRQKEPKGLGDAIYCARHFIGDEPFAVMLGDDIVDNDVPCLKQLTDAYEEYRTTILGV
                     QKVNQEDTNKYGIIEAKNIEGRVYKVKDMVEKPESGKAPSNIAILGRYIITPEIFDIL
                     KDLPPGKGGEVQLTDALKILSKKEAMYAYNFEGKRYDVGDKLGFLEATVDFALKKEDL
                     KEDFIKYLKHVCSEFDKNNNVLYNNTDEKIDLIEVNEARELEIQK"
BASE COUNT        12194 a         3117 c         5422 g         9129 t
ORIGIN      
        1 acaatgataa aactaaatga taatttataa atatctcctt acgatatata ataattgtag
       61 ggagatgttt ctttttttgt atagcataat ttttctagaa attctgtttc aatatgtaaa
      121 aagttggtat atataaatag tataaacagt gttgctaaaa tatggtagat attatataat
      181 ataagtggat atgtatatac attatgagtt atataatttg aatttaataa aatagaaacg
      241 ttgatttatg ttctgtaatg tgggcacctt ggagcatatt gagttagtgg tgcaaccggc
      301 tatgaaattg tatttattta taaatactat tattttcata gcttttttta tttaaaaaat
      361 ggttatatgt tagaaataaa tttggttagt acgtttaaat aattttactt agatttttaa
      421 ccagatatat tgtaagtaag tattaattaa aaaatttaag ggggaataaa aatgaagtta
      481 aaaaagaata aaaaaggttt cactttagtg gaattattgg tagtaattgc aattataggt
      541 atattagcag tagtggcagt tccagcttta tttagtaata taaacaaggc taaggtagca
      601 agtgttgagt ctgattatag ttcagttaag agtgcagcat tatcttatta ttcagatact
      661 aataagatac cagttacacc agatggtcaa actggtttaa gtgttttaga gacttatatg
      721 gagtctctgc ctgataaagc tgatataggt ggaaaatata aattgattaa agttggtaat
      781 aaattggtat tacagatagg tacaaatact gaaggagtta cattaacaga ggcacaatca
      841 gcaaagttat tgagtgatat aggtgaaaat aaaatatata caagcactac aacaaatagt
      901 ttgggaaatc cattaacaag taatacaaaa atagataata acgttctata tatagtactt
      961 atagataata ctgtgatgga cacaacaaaa tagttaaaac aatattttta taaaaatatt
     1021 gtaaatttaa tgaaaaatat aatataaaag acaggaagaa aatcttcctg tttttttata
     1081 ctattttctt taatgttcaa ttatgaatga tgatataatt gagatagtaa aggagtgaaa
     1141 cctgtggcta aaaaagttag aataggagac aaactggtcg aaaaaggata tataacagaa
     1201 gaacagctta aatgggcact atcagaacaa aaaaacagtg gaaaaagact aggagaattt
     1261 ctagtacaag aagggcttat agatagtaac ctactaataa gtgtattaaa agaactcttg
     1321 gatatagaaa gtatattttt agaagggaca gaaatagaca ctttagctac aaagatggtt
     1381 ccagaaaata tatgcaaaag atatactgta tttccattca aaatagatgg aaataaaata
     1441 tgtctagcca tgtcagaccc acaagacaga gaagcagttc aagatgtaag gcgtatgtca
     1501 ggtaaagacg tagagatatt tatatcaagt acagaagata taaacaaagc aataggtcat
     1561 gcttatgcac attcagaaat aaataaggca atgactgagt acaacaaaaa tagaactggt
     1621 ggagtaagag aaacagtaat acttgaagaa gatgtaaatg ctgctccaat agtacgatta
     1681 gtaaataaca tactagaaaa tgctgttaga atggaagcaa gtgatataca tatagaacaa
     1741 agtgaaaatt acatgcgtgt taggtttagg atagatggaa tgttaagaga atatatgagg
     1801 atgaactcag caccatataa ggcagtaatc agccgtatca agataatgtc agatataaat
     1861 atatctgaaa agagaatacc acaggatggt agaatctatt taaaagtgga taataaaccc
     1921 atagatttta gggtttctac aatgccaact aacagggatg aaaagatagc tatgagggtt
     1981 cttgataaga gtaattttat ggttagtaaa gaagtactag gaatagacga acatggctct
     2041 aagatatatg atgaattgat aaacactcct tatggtctta tattggttgt tggccctact
     2101 ggaagtggga aaaccactac attgtattca atgttaaatc aactaaatac agaaaataga
     2161 aatctattaa caatagaaga ccctatagag tatgaattac ctggtgtaaa tcaatctcaa
     2221 ataaatgaaa aggcagggct tactttcgcc agcggactta gagcttttat gcgtcaagac
     2281 ccagatataa taatggttgg agagataaga gatactgaga cagcagaaat agcaattaga
     2341 gcttctctta caggacattt agttttaagt acattacatg caaatacagc agttggagca
     2401 atttcacgtc ttttagatat ggatgtggaa agttttttaa taacttcatc tgttttaggt
     2461 gtaatatctc aaagacttac acgaaaaata tgtgagcatt gtaaggtttc atatgaagca
     2521 gatattggag aaaaaaaggc tcttggtatt gatgtaaatg aatcagtaac tatttataga
     2581 ggaaaaggtt gtgaaaggtg taataatact ggatataaag gacgtctagg tatatttgaa
     2641 atgcttgaaa ttactccaga aataaaagag ttaatagatt catctgcaaa tcaaagagaa
     2701 attttaaaga tggctagaaa acaaggtatg gttagcttaa aagaggatat agttaaaaag
     2761 gttttaaatg gaaagacaac agtagaagaa atgattagaa taattttgat gactgattaa
     2821 aggatgatat tatgaaaagt tttaaataca cagtaataaa agaggacgga aaaaagcaaa
     2881 ctgatgttat tgaagccaac gattttaatg aagctagaaa tcttcttaga aagagaaatc
     2941 ttagagttat tgaaatcaaa gaaagcaaaa ataaaaatat aagattatct agtataaaga
     3001 gaaaaaaaga cttaggagct gaccagataa gccacttttg tagacaattt gctattatag
     3061 tatcttctgg tataaatagt atttctggat tagaaacatt agctagaagg tcaactaaca
     3121 ttacattaag agaagaaatt aatcgtatag tagcagaaat aaaagtaggt tctactattg
     3181 cagattcaat gctttcatca aaatcaaagt ttccaaagtt acttggtgct atggtagcta
     3241 caggtgaagc tacaggtaaa ttagatgaag tattaaaaag tatggcttca ttttatgcaa
     3301 gtgaacacag ggttaaacaa aagcttagaa atgctgcaac atatccatta atagttttga
     3361 tttcttcatt tataatgatt tttatattta caacatttat ggtgcctaag atgattaatt
     3421 caattacaac agtaggagca actcttccac ttataacaag aattgttatg gggtttggta
     3481 tgtttatgaa gaagttttgg ataatagtac ttttagttat tatattattt atctatcagt
     3541 ttaaaaaata tctaaaaacg tcagttggaa gggctcataa agatagagtt ataaataaga
     3601 tacctgtttt aggaaaagga ataaattgta tggtagctac acgattttca agagcattgt
     3661 atctatttgt atcaacagga tacccattag ttcaagggct tgattatatt atagatagtg
     3721 taaataacac tatggcagag aagattttag catcagctaa agatggaata acaagaggag
     3781 aaggcttagc agaaaattta gagagatata cgtattttga ctctgtgttg gtacagatga
     3841 tagctatagg ggagcagaca ggggaattag agaatataag tagacagatg gcagaatttt
     3901 atgagtatga atccgagatt tatttaaata ggatggcctc aatgatagag cctgttttaa
     3961 taattgcagt tggtattata gttgcgatac tggttgtatc aatattcatg ccaatgctta
     4021 gtatatatga tgccatgtag gagggaaagg acaaatgaac aaggtttatg tatcatatta
     4081 tggcgattct atttccatag ttgagggcag atataaacac gataaggata agttttatgt
     4141 aaaaaatttt aatgtgtttt caatagagga tattgcacaa gatttttcta aggaaaagta
     4201 tgctctattg agatacgcat tagaaaatgt taagttaaaa tcaagacatg tagttttttg
     4261 tttaaataca agagatgtaa ttttaaaacc ccaaaaactt cctaatatgg acaaaaagga
     4321 tttagacgga tttatgaagc tagagatgga cgaaatgatg gctttggagt cagatgaata
     4381 tgtattttcg tatgaagtat ctagtgaaac acctgataat tcagatggcg atgaatcaag
     4441 cttaaatctt attattggag ctatacaaaa ggaagaggta aatgcaattg tagagataat
     4501 gcatgaattt gatttagtat tagatagaat agatacatta tcaacagctt acttaagaat
     4561 gcttaaaaat ttagactaca atgatataat ggtaaccaat ataagtgaca atagcacaat
     4621 tataaatata tataagaaag atactttatt tattagtgat aatataccta taaagataac
     4681 tgctaaagat atggacatgc aattattaaa tgtagtagaa gaatcaaaag gtcttatgaa
     4741 ttactattcg tcaagaaact ttggtaaggt tacggatact atattaatat taggaaaaag
     4801 atattccaat aaattaattt atgaaacttt tagctctgca ttttcaagct atgtttctca
     4861 aggccttact gagataatgg atatatctga tatgataact ggagatatag atgaagatga
     4921 tataaattca attgtagagc ttttaggaag tatgatagaa cctaaaggga aaagagattt
     4981 tgaaaatata aactttttac caatggatat acttagaaaa cagataaaag ctaaacaaat
     5041 gaaaaacata ctgaaaattg ctccaatagt tattttgatt cttgctatac cttatttatg
     5101 tttaattttt tcaaataatt tagcaaataa ggaacttgaa aatgttaatg gtgaaataag
     5161 aaatattgaa tctgcatatt atcagattgg aaatatagaa aaaatgatac agagtaaaag
     5221 cgaagaaata aaggtatatg atatgttaat tgggaaaaag gtaaagtggg aaaatttatt
     5281 ggatgatata gataaaaata ctccaagtaa agttgaacta acaagtttat caacaactta
     5341 tcaaaatctt ggaactaata ataaagctga taattcaaaa agtaataaga ctacaacaga
     5401 taaatcaagt aacaatgaaa caaaatcaag tacgaaaagt gatagtaatg gagataaaac
     5461 agataatgat ttaaatgaag ataagactac aaatgtgtca gaagcattaa ctggaaatag
     5521 tgagactaat agtaataaca cggataaaga tgataaaact ccattgtatg ataagatacc
     5581 aaatgttata aatatagaag gaaatgctaa agattcctct tatgtaggtc aatttttata
     5641 taaattaaag ggattatact atttcaagga tgttaaatta catagcataa aaagtaatgc
     5701 agaagattct aactatacat ttagtatgac attatacctg aaagaaggtg tattaactaa
     5761 tgaataaaga tatatttaaa aaagatataa aagatatatt taaaatggaa gttagtctaa
     5821 aagggattct tataaaggtt ataattttat tagctatttt atcatatggt tattttggac
     5881 tttaccctca atatcagaaa tatattgcta caaaaaattc tgtaaaacag gctactgaaa
     5941 aattaaatat atataaagag aaaactgcac ttatgccaaa atatgaaaaa caacttgaag
     6001 aagttgaaag tgaattaaaa gaaaaaagca aaaatctttt atatgatatg caagatggtc
     6061 tttttttaat aggtttagat aaaaaaatga aggcttataa tattgactta accagttatg
     6121 acgttggaaa tgttaaagaa tacgaacatt tttatggtat atcaacaaca ataagtgtta
     6181 gaggtgatta tagaaatgtc aaaaaattaa tggcttattt agaagaacaa aaaaatgtta
     6241 ctcaaatact agattataca atgacttcat atatagaacc agaaaaagaa gaggaagaaa
     6301 caacaaacaa taaggattta attaaaaatg tgaacataaa taaagataca aaaatatatt
     6361 gggtggaagg cgaaaataag tggcatttat ataaggattg tccattgata aaagagggag
     6421 agattataaa aaatgacaaa cttttagata ggtctactaa ttatctatgt aatacttgtg
     6481 tacttagagc tgcaacagaa aaagaagcaa aaaaagaagt cgtagctcca aaggcagaag
     6541 gtgtagtaga tgctacattt aaatttataa tgtacactaa agagaatact acaatgatac
     6601 ttgatacaga taatccatct tcatggagac caggaaaata taatccattt aagtctagtg
     6661 cgaaataaag ctaagtaagc tttaaagata ggtgatgagt ttgtacaaaa ataatgaaaa
     6721 aggtcttact ttattagaag taataatagc tgtttttata ctgacaatag ttttaagtat
     6781 ttcttataaa gtgttcaatg gtataacatc agcagtaaaa aagcaacaga ttattacaga
     6841 tgctcaagta aatattaatc taattaataa gtatctaaat agagatttgg aaaactgtaa
     6901 agagctaact aaaactggtt caggtaataa ttatgaatac aatatagaga tgccagataa
     6961 tgttgtaaaa tatgaagttt ctatagaaac taaaaaaaat acagaggtat attctgtaac
     7021 gagaatccaa aacaatacaa ttgatacaga aaatgaagtt agagaagaga taatctataa
     7081 tcaaccacta gttcaaaaca ataaagaaat gaaggaaaca ccatttaaaa tagaaaagca
     7141 aactggtaaa tctatttata ctgtaagtat atactataat gaatcagtgc aagaaagtca
     7201 taaaaatagc aatttaaata acaaaactta tacatttgat gttatgtcaa gaataggata
     7261 ataagaattt attaaaaaat aagtaggttg ataaaatatg agtaaaaaga atagtgaaag
     7321 aggattttcc ttaattgaag tgttagtagc tatggctatt atgggaattg tactatttgc
     7381 attttttaat attatcaata ctaataacaa agcaaacact aaaaatgaca cagatataac
     7441 ttccttaaac tatgtccaaa gtgaaataga aaatctaaga gaaaagataa aaagtggaga
     7501 atttgatttt gatagtttag ataaactaga agatggaact gtcgtatatg aaaaattaat
     7561 agataaatca aaaaaagttg tatatgataa agttcttagt gaaggtgacg tgagcttata
     7621 tgatactcca tatgaaaaga ttacaacaat aaaagatgaa gatggtaatt taatcgacaa
     7681 aggaaatatt acaaataaga taaagactat tgtagaagat aaaagtggtc agatatataa
     7741 aatagctgta actgggaaaa gcatgaatga ctattcaagt aaaaaagagg ttaaaattgt
     7801 aactgaaata tttaaagata aataatttta cttgggggtg agattttgag gaagtggaat
     7861 aaatttaaaa gtgaaagagg ggcagcttta gttttagttt tggttgtagt agcattgctt
     7921 tctattgttg gattgatatt ttcaaatcag atagctaata gaataaaatc tacaaagact
     7981 actaatgaag gtatacaagc aaagtattta gcagagacat gtgtggaaaa tagtattgat
     8041 aaagcatatg aaaaattgta tgatgagctt gagaaaatgg ataatgagtt taaaagtgaa
     8101 aatcaagaaa aatccatatc taggtcaaaa ttaagaaata tatcagatga agattttaat
     8161 aatcaagatg aggaaaatat tgaagcagaa agacttggat atatgaataa tataaatttc
     8221 tatttaaata aagcttcttc agacttggaa aaagctagta tggaattgaa aaagctatat
     8281 gatttagata tgttggacta tagagacatt gaatatgtag atgcaaatat tatttcacat
     8341 agagattcta tcttagaaat ttgcaagaat tatacttctg gagatattag taaaataaat
     8401 gaatatattc tgaaagaaga tatagactca actacattga ttgaggcaaa gttagtgaat
     8461 aatgatattc ttttaaaaaa gtttttagaa gaaaataaaa ttgaaaatga acatttaaat
     8521 tcagcctttt cacatactta taaggcatta gataacattt ctttggcgat gcaaaatatg
     8581 attgagtata gacatacgtt tcatatagat gagccaaaag tagaagtatc taatgggatt
     8641 ccagactctc aacaatatta tgaactaata caaaatccta taataaatag tatggagtat
     8701 agctggaata gtaaatggga tacattagaa aacctattgg aaattttacc aaatcaaact
     8761 caaggattta attctttaag agttcatttg agaaataatg taagaaaatt tgagaagtta
     8821 agcgataata tatctagtgg taaaaaaaat acagcaaaaa atttcttaaa gtataaagag
     8881 ttattgtatg aaatatcaga ccaatgtaat caactaaaaa gtatgtctta tgagaaaata
     8941 cctgttaaat atgataacat ggctttgata acaacttttg actatataca aaatgagctt
     9001 ttagcagaga taaaatgcag attaaaagaa ttaaaaccac aagaaattga taaaacagaa
     9061 ggtattacta taaaaatacc tttttataag gctgactacg acatgacaaa agaaggatgg
     9121 cctaaattaa aagaaaatgg ttcaggggcg gagttaagct taatggtaac tggtgataaa
     9181 aatggaataa aagaagtgga agttacagat ggtaaaaaaa atattattgg tttaggtgtt
     9241 gaagaaaatt caaattcaaa gtataaggtt gacgccattg taaactttaa tctaaatata
     9301 gatacaaatg tagttggaaa ttatgatata aaagataaaa ttttgattaa tcatgatata
     9361 agttcataca aaaaagtaaa ctaaatataa gaagcaactt agtataaata acaaaataat
     9421 tttattattt actactaaaa aaataagtaa actaatagaa gatatggaga gtaaatatta
     9481 tgtatattta tgatttatta gagcaaggta taagactaaa cgcatcagat atacacataa
     9541 cagttggaac aaatcctgta gctagagtaa aaggtggatt tgtaaaacta agtgaacaaa
     9601 tattgacatc agaagtaaca atgcaaatgg caaaagacat agcaggggaa tctatgttta
     9661 aagtaataga agaacatgga gaagctgatt tttcagcttc tctaaaaaca ggagaaagat
     9721 ttagggtaaa tgcatatagg caaaaaggaa attacgcaat agcaataagg actataacag
     9781 cagaaattcc aacatttgaa aaattaggcc ttccagagag tataaaatct tttactgaaa
     9841 agcataaagg attagtttta gttacaggtc caacaggaag tggtaaaagt acaacacttg
     9901 caagtatgat aaatataata aatgaaaaac aacaaaaaca tataattaca cttgaagacc
     9961 cgatagaata tgtacatcat cataaacaaa gtttagtaaa tcaaagagaa gtaggaactg
    10021 atacagaaag ttttcattca gcccttagag ctattttgcg tcaggaccca gatgtaatac
    10081 taataggaga gatgagagac cctgaaactg tttcaatagc attaacagca gcagaaacag
    10141 gtcatttagt attttcaaca ctacatacag taggggcagc taagacaatt gatagaatag
    10201 tagacatgtt tcaaccatcc caacagcaac aaataaaaac acagttatct acagtttgtg
    10261 aaggtgtggt atcacagcag ttacttccta cagcagatgg gaaaggcaga atagcagcaa
    10321 ttgaattgat gtttgctact ccagcaataa aaaacctgat aagagaaggc aaaacatatc
    10381 aaattccaaa catgatacaa actggagtta aagtaggtat gaaaactatg gaccaagatt
    10441 taatggaact ttataaaaat ggaaagatta caaaagatat ggcacttagt aggtgtactg
    10501 accaagagtt tatgacaaga atgataggtg gagtaaacta caatggatat tataatcgtt
    10561 aatctatatg tatttatagt gggtatagtg tttggaagtt tctttaacgt ctgtattttt
    10621 aggattccag aaggtatatc tatttctaga ccaccttctc attgtatgag ttgtaatact
    10681 agactaaagc caatagactt aataccagtg ctaagctatt taatgagtgg cagaaagtgt
    10741 agatattgtt atgagaaaat ttctagtaga tatgctatag tggaattaat tacaggactt
    10801 ttatttttaa cagtatttat gttatatgga atatctacat caactattta ttatttggtt
    10861 cttgtgtctt tactgattat aattacattt atagatattg accactttat aataccagat
    10921 aaaatattgg tttttggagc tatattttct atgattttta accttttatt taaagaaatt
    10981 ccagttaagg attcaattct aggtgttttt atttgtggag gaagtgtctg gattatagtt
    11041 cttttgattg aatttgtaat aaaaaaagag tgtatgggtg gaggagatat taaactattt
    11101 gcaatgctag gcctttacat gggtgttaaa aatggtcttt tgacagcctt actaagcgtt
    11161 tatgtaggag cagtctatgg aatctgtgta ataattataa gtagaataaa aggaaaggaa
    11221 tataattctg ttataccata tggacctttt atatctattg gagcattaat aacaatctta
    11281 tgtggaaaga aattacttga actttatttt tctatggtta tatagatgat agtcaagtta
    11341 aataaagttt taatttaaaa aaatgaatta aagtatttaa taaggacaat attattatta
    11401 accctattac taaagtgcta caaactatgg agtatataat aataatttcc ttattttttg
    11461 cacatttgat aaataaatct ctatgtgttt tttgtgatag agaaaagaaa aatttataca
    11521 atacgttttt tatttacata ataactgtag ttttatctgt attaatatat ctaaaatttg
    11581 ggattagtgt agaatgtgta caatatatgg ttttaattcc atttttaata attatatcca
    11641 ttgtagatta ctacacaata tatgtatatg atataacaat agttagtggt ataatagttc
    11701 aaggtataat ttttttggcc tttttcaata tagctcaatt tgattatata aatcatatat
    11761 ttggactatt gataggcttt attatacctt atattattgc taaactaaca aaaggattag
    11821 gctatggaga tgtaggtctc tatagcttat gttgctttgc attgggaaat aattatgggt
    11881 tttatgtgat agttttatca tttattttag cgtttttgtt ttttattatt ttatacataa
    11941 ctaaagtatt taaagatgta gatataagaa agcaaattcc ttttacacct tttatttcct
    12001 tgtcgacaat tttaataata tttacagagt ataatatatt taatttttat tataatttgt
    12061 tatacaaatt tatataacag gaggtaagaa atgtacgtag tagtagggtt aggtaatcca
    12121 ggaaagaagt atgagaagac tagacataac gttggatttg atgttataga tattcttgct
    12181 aaagaataca atattagtgt tactaaaata aagcataaag ctttgattgg tgaagggaga
    12241 gtaggtactg aaaaagtatt attagttaaa ccacaaactt atatgaattt aagtggagaa
    12301 acattaatag atatatataa atattataag gttgatttaa gtaatatagt agtagtatat
    12361 gatgatatag atttagaagt tggaaagata cgtataagaa aaaaaggaag tggaggaact
    12421 cataatggca tgaaatctat aactaattgc ctaggttcaa atgactttcc aagagtgaga
    12481 gtaggtgtat ctaaaccaga agctggtcaa gacttagcag actttgtact ttctagattt
    12541 agaaaagaag aatctgataa cataaatgaa gcattagaaa aagcggcaga tgctattgac
    12601 tcaataataa gagaaaatat agatatgtct atgaacaaat ataatggata gtgagaggga
    12661 gatttaaatg aatgatgttt ttttatatcc tttgcaaaat tcaaaagagt ataaagatat
    12721 aataaactgt ataaaaaata caaaaggctc attattggtt aatggacttt taccagtaca
    12781 aaaacctcat atatcgtatt ctatatttaa tgatttatca cgacaaatga tttttataac
    12841 aagtagtgat ttagaagcaa agaaagtata cgaagattta agtttttata tggaagataa
    12901 agttgaatat ttaggttttc aagatattta tttttatcac ttggatgcaa aggacagaaa
    12961 cgaagaagcg aagaaattaa aagtattgtt aaaactagca aataaagaaa aaatcatttt
    13021 agtaacttct attgaggcag ttttaagaaa atacatacca aaacaagttt tgctcgatag
    13081 tgtatctcat tataaggtgg gagatagctt agacttagaa aagttaactg agaaattagt
    13141 ttcattggga tatgaaagag tttcaaaaat agaggggttt ggtcaattta gtataagagg
    13201 tggaattatt gatgtatttt cacttgaata tacaaatcca attcgtatgg aattatttga
    13261 tgatgagata gattccatac gaacttttga cgtatattca caaaaatcaa ttgataaatt
    13321 gcaacaattc tctataactc catctaggga atttatttat cccgaaaaaa ctacagatgc
    13381 attagttaaa ttaaaaaaag aaacaactaa aaatactgat gaaggtacat ttcaaaatat
    13441 agattatata tcaagtaaga cttattttga aggtgtagag aactatatag attatattta
    13501 tccagaagaa aacaaaagta tatttactta tttagcagat gatgcaatag tatttattaa
    13561 tgatataacg agacttaaag aaagatgcga aaattatata aatgagttta gagaaaatta
    13621 taaattaaat cttgaaagag gtctggctat taaaaaccaa ggtaaattgt tatatcacta
    13681 tacagattta gagtacttag taaaagacaa gtctgtggta ttaaactcgc tattgccaaa
    13741 gtctataaat aacttttcta taaaatctat aataaatttt gaaagtagag aagttcctac
    13801 atttaatgga aaggtagatt tgcttgtaga agaattaaat cggctgaaat ataatggata
    13861 caaaataatt ttagcaacaa ataccttaga aagagcaaat aaattaggaa aagatttatt
    13921 ggacaaaggc ttagaaacga ccatttctaa agatagagat attgaaatta aatcttcaca
    13981 agttataatt gtacctgcac acataaacag tggttttcag tataaatcta taaagtttgt
    14041 tgttataaca gataacgaaa tgataggtgt ttataaaaga gcgtctaaga ctagcaataa
    14101 aaaagttaaa aagggaaaga aaatagaatc ctttttagat ttaagtgttg gagattatgt
    14161 agtccatgaa aatagtggtg taggtagata cacaggaatt gaacaaatta cagtcaatgc
    14221 tattaaaaaa gattatatga aaatagtata tcaaggtgga gataatctat atgtgccaat
    14281 agaccagatg gacaaagtac aaaaatatat tggtgcagaa gtagaaaaag ttaagttaaa
    14341 taagcttgga acaaatgagt ggacgaaagc aaaagcaaaa gttaaaaaag aaattgaaga
    14401 tatgacaaaa gacttgattg agctttatgc aaaacgtgaa aaaatacaag ggtataagtt
    14461 ttcaaaagat acaccttggc aggcagagtt tgaatcgttg tttccatatc aagaaacaga
    14521 agaccaatta aaggctatag aagaaactaa aaaagatatg gaatcaagta aggtaatgga
    14581 taggctagta tgtggagatg ttggttatgg aaaaacagag gtagctatta gaagtatttt
    14641 taaagcttgt atggaccaaa aacaagttgc tgtattggtt ccaacaacaa tattggccca
    14701 acaacattat aatacattca aagagaggtt tgaaaattat cctttgagag tagaagtttt
    14761 aagtaggttt aaaacaccaa aacaacaaaa acagattatt gaagatgcta aaaaaggatt
    14821 agtagatatt ttaattggaa cacatagaat aatatccaaa gatataaatt taccaaactt
    14881 aggtcttgta gtcattgatg aagaacaaag atttggagta aaacataaag aagctttaaa
    14941 gaaaattaaa tctacagtag atgtacttac actttcagca actcctatac caagaacatt
    15001 acatatgtct ttaagtggaa taagagacat gagtgttata gaagaacctc cacaagaaag
    15061 acatcctgta ataacatatg ttacagagag taaggaaagc gtaattcaag atgagataga
    15121 gagagaatta agtagaggtg gacaggtatt ttttgtatac aatagagttg aacacattga
    15181 agaaatggca agtatgattc aaaaactcgt accagatgct agagtggctg ttgcacatgg
    15241 aagaatgact tctaaatcat tagaaaatat aatcctagga tttttaaata aagattatga
    15301 tgtactagtc tgtactacaa taattgaaac tggtatggac atatcaaatg caaatacaat
    15361 gattatatat gatgcagata aaatgggatt ggctcaactt taccaattga gaggtagggt
    15421 tggaagaagt tcaagacaag gatatgcata tcttttgtat gaaaaagata aaactttaag
    15481 tgagatagcg gagaagagat taaaagctat cagagaattt acagagtttg gttctggttt
    15541 taaaatagca atgagagacc ttgaaataag aggagcagga aatattttag gttctcaaca
    15601 acatggtcat atggcagtta taggttatga tttgtacgtt aaaatgctta atgatgcaat
    15661 taaaaaagtc aaaggtgagc ctattgtgga ggaaattgat gttgaaatag atttaagtgt
    15721 aaatgcatac attccagata attatatcaa agatgagttg attaaaattg aaatgtataa
    15781 gaaaatagct tctattgaaa acaaagaaga catgttggat atacaagaag aacttgagga
    15841 tagattctct gatataccta aaccagtcca gacacttctt acaatagctt atataaaatc
    15901 gttatgtaag atattaaaaa tagaaaagat aagacaatta aaagatgaaa tattattagt
    15961 tccaataaca aagtatagaa ctaaacagaa aataggttat aatatagtta cagaattaga
    16021 agaattatta gaaaagatgt gcaaggtaaa ataacttgta aatttaagaa gggatgtttt
    16081 aaatttgaaa aaagtaataa cattggtaat agctatgatt ttggtagtat ctgtgactgc
    16141 ttgcagttct agtaaaggag aaactgtggc aacagttgaa ggtacaaaga tatcatcaga
    16201 tgaatttaaa aaaactatag ctctttataa agattctatg gaacaaactt atggaaaaga
    16261 tatatgggat aaggaagttg aaaagggtgt aaaatacaag gacaagttta aagatttaat
    16321 tcttgaccaa cttataacta cagaagtaat atattctcaa gctaaaaaag ataacttact
    16381 tcctaaaaaa gaggatgttg aaaaaagttt taaagaatta aaagacgcta tgggtaaaga
    16441 tgaaaaatat aaagaacaac ttaagaaatt aggtatagat gatgagtttt taagagacca
    16501 acaagagaaa gatttagcaa tgcaaaacta tcaaagtaac tttgctaaaa agactaaaat
    16561 atcagatgaa gaaatgaaaa aatactatga tacacataaa gatgaattta aaaaagatga
    16621 ggtagaagct tctcatattt tattgaaaac agtagatgat aataacaagc cactttcaga
    16681 taaggaaaaa gctgaagcta agaaaaaggc tgaagaggct cttaaagaag ttaaatcagg
    16741 tgaggatttt gcaaaagtag caaaaaaata ttcacaagat acttcagcaa gtgatggtgg
    16801 taaattagga ttcttttcaa ggggtcaaat ggttgctgaa tttgaagatg ctgctttttc
    16861 tatgaaaaag ggtgaagtat ctgatttagt tgaaactcaa tatggatacc acataattaa
    16921 agtgacagac agaataaatg agcaaacatc atttgaagat gctaaagaaa ctataaaaga
    16981 ccaactgttg aaaaataaat atcaagaaca aatagaaaaa ttaactaaag aagctaaggt
    17041 agaaaaagat gaaaaagtaa taaataaaat cacaatctaa gacttaaaaa aagagtaata
    17101 tgcttaaaaa agctatatct ggataagata tagctttttt tgtatatttt tcttaacaat
    17161 ggtaaaaata aacttaaagt aatttatgga gggattaaaa aatatgaaag caacaggtat
    17221 agttagaaga atagatgatc ttggaagggt agttatccca aaagagataa gaaagacatt
    17281 aagaattaga gaaggagacc ctttagagat attcacagca aaagatggag aagtcatact
    17341 taaaaaatac tctccaatag gtgaattaaa tgaattttcg caagaatatg ctgaaacatt
    17401 agctgaagta ctaggatatg gagtgttgat aacagactta gattctataa tagctgtgtc
    17461 taagcttcct aaaaaagatt ataaagaaaa aagtataagt gatgagttag aagcaataat
    17521 aagtgaaaga acagtaaaat attcaaaaga taaaaacatt ttaccattat tcaaggaaga
    17581 ccaaaaagaa tatactagtc aagttatcat gcctataatt agtgcatctg gtgactgtat
    17641 aggttcaatt gtagttgttt caaaggataa agatacaatt gcagaaagtg aagaaaaatc
    17701 tttaaaaatt gctgctaact ttttaggaaa acaagttcaa taattttatc ataaagacct
    17761 tttacatgaa ggtcttttga agtttttgtg taaaatattg aatatagcat taaaaatatg
    17821 gtatattaaa gtttgttata taaacagtat ggaggttatg ttatgaacaa taacaaaaat
    17881 aaagactcct ttctaaaagg agcccttata cttgggctag caggtgtaat cgtaaaaata
    17941 atgggtggat tttttaggat tccccttgga aacatgattg gttcagaagg aatgggatac
    18001 tatcaggctg cttatcctgt gtatactcta tttttaacat tagctactgc tggatttcca
    18061 acagctgtgg caaaattggt ttcggaaaaa gttgcaattg gaaattttaa aggtgcaaat
    18121 aagatattta aggtatcaca tacagtttta ttcattaccg gtataatatc attttgtata
    18181 ttattttttg gagccgacta catagtaaca aacgtaatga aaaatccagg tgcgttatac
    18241 tctatgaaag ccattgctcc agcattacta tttgttcctg caatgtctgc atatagagga
    18301 tattttcaag ggagacaaga tatgactaag attgctgtgt ctcaagttgc agagcaattt
    18361 tttagagttg tattaggtct tactttggct tacttgctta tgaagagtct aggtcagact
    18421 tttggtgcag caggtgctat atcaggggct acaataggtt caatagcatc tatgctttat
    18481 ttggtgtttg cttatatgct agggagaaaa gagcgtagag cagagataga tgcaagccaa
    18541 agatttaaag atgaaagagt gtcttatatt tttaaaaagc ttttaactgt tgccattcct
    18601 ataacaatag gagcatcagt aatgccactt gtaaatatga tagataatgt tattgtaata
    18661 agaagactta tggaagcagg ctttacatac aaagtggcaa attccatgtt tggtcaatta
    18721 acaggtatgg caatggccac aataaattta ccagcagtca taacaactgc aatgagtatg
    18781 agcttagttc cagcaatttc taaagcatat gcattaggaa ataagtcaaa agctagaaaa
    18841 gatactaaga gtgcagttaa ggtaacttta cttatagtat tgccttgtgc gtttggtatg
    18901 gcttctttag caataccaat aatgggtctt ttatttccac atgaaccatc ttctgtagga
    18961 actattttgt ttactcttac tccatgtgta ttatttttag gattaataca gacattgact
    19021 ggtataattc agggaatggg aaaaccaata gtacctgtaa ttgctttatg tgttggtatg
    19081 ctatgtaaaa ttgtaataag ttatacttta acaggtatac cagatattaa tgtactgggt
    19141 tcagcatttg gtacagttac agcatacttt gtggcagcca tgattaactt actatatgtt
    19201 aaaaaacaca tgaatgtaaa cttttctaaa aaagaattta taataaagcc atttataaca
    19261 gttatgacaa tgtttataat ggttaagttg agttatggtg ctttagttgg attcttagga
    19321 aacagcatat ctacaataat tgcaatatgt gttggtggaa ttgtatatgt agtagtaata
    19381 cttggaatag gtggaattaa aaaagaagag attctaacta tgccaaaagg tgataaatta
    19441 tataaattgt taaaaaaagt taaacttata agataatata ggaatatata tgcaaataaa
    19501 ggaaatacta tacaaatagt aaatctcctt atttgtataa taatataaaa ataaagtgaa
    19561 atttgctaat aggagtgatg ttatgggtaa gataagtatt gtaggtcttg gaccaggaga
    19621 ctattctctt ataagccaag gtgtgttaga atctttaata aatagcccta aagttttttt
    19681 tagaacagag aaacatccaa taattgataa acttaaagaa actatacaat acacttcttt
    19741 agactacttt tatgaaaaag aggaaaattt tgataatgtt tatttgaaaa tatctgaatt
    19801 tatcatagaa gaatcagcaa agggtgacct agtgtatgct gttcctggtc atccaagagt
    19861 tgcagagaag actgtaagta taatagagag attagccaat gaaaagcaca tagcagtaga
    19921 aatattgcct tctatgagct ttgtagacgc tatgtttaac tatttggcta tagacccatc
    19981 tgagggattt aaactggtag atgcttttga aatagaaaat tcatatatag acttggatac
    20041 aagtatgata ataactcagg tatatgataa atttatagca tctaatgtaa agttaaaact
    20101 tatggaacat tatgactata atcaagaagt atgtatagta aatggagctg gtgtcaaaga
    20161 tttagaaagt aaaaagttta taaagctttg tgaattagac agaaatgaaa acttttttga
    20221 ctatttaact agcttatata tacccaaaag ttctaaaaag atgtataata cagtacatga
    20281 cttagaaata atagttaata cacttagaag tccatctgga tgtgaatggg ataaaaaaca
    20341 aacacatcaa tcattaaaaa attctgttat agaggaagca tatgaacttt gtaatgcaat
    20401 tgataataat gatatagatg aaatggtaga agagttaggt gatgttcttt tacaggttat
    20461 attccattgt cagataggaa atgaggaggg ctactttgat ttaaaagaag tagtaaatgg
    20521 catatgtaaa aaattaattc atagacatcc tcatatattt agtggtgcaa aattagatat
    20581 gtctaagttt gagaaaactt gggaagaatt aaagaaagaa gagaaaggcg aaagtactat
    20641 aacagatggc cttagaagga taccaaaaca tttaccagct cttatgaagg ctagtaaagt
    20701 tcaacataaa gcagcacttg tcggctttga ttgggacaat gtagaagatg tatttaaaaa
    20761 aattgaagaa gaatatcagg aattacttga cgaacacagg gtcgggaata taaaatacat
    20821 aaaggaagag ctaggcgatt tattattttc gatagtaaat ctagctagat ttttagacat
    20881 agactcagga gaagccttga actgtactac agaaaaattc ataaatagat ttgactttat
    20941 agaaaagagt gcaatgaact tgaataaaaa attagaagac atgacacttg aagagatgga
    21001 tgagttttgg aatcaggcta aaaaacaata aaattgtgga aggaaatttg tcaaaaatct
    21061 agaactttta tcacaaagat agaagtctat attataaaca tttttaggag gtaaatatcg
    21121 tgaataaagc tgaattagta tcaaagatgg cagaaaaaag tggattaaca aagaaggaag
    21181 cagaagctgc gttaaacgca tttatgagtt ctgttcaaga tgcactagta aataatgaaa
    21241 aagttcaatt agttggattt ggaacatttg agacaagaga aagagctgct agacaaggaa
    21301 gaaatccaag agatccagag caagttatag atataccagc ttctaaagca ccagttttca
    21361 aagctggaaa aggattaaag gatataataa atggataatt agaaatcttg tgaaaaagtg
    21421 tggataaatt tgccacactt tttctataga accaaggaag gtgataatta tgagattaga
    21481 taaattttta aaagtatcta ggattataaa aagaagaact gttgctaaag atgcatgtga
    21541 taaagggatt gtcactataa atggaaaagt tgctaaatct tcttcagagg tgaatattgg
    21601 agatacatta gaaatacaat ttggagagaa gaagatgaag tttaagataa atgaagtaaa
    21661 agagcatgta ttaaaaaatg atgcaaaaga aatgtacgaa attttagaat aaaattaata
    21721 gtcctctagt atattaatag tagtattatt gaaatattta ctggggggat attatggaac
    21781 agaacataac tttgaaagat agatcaaagc tggttatttc gggagtcgag cacatatact
    21841 cttttaatga caaaagagta gagctcaaaa cttctgtagg cgagatggta attgaaggcg
    21901 aaaacctaga tatgagcaag ctaagtatag acgaaaatat aattagcata gatggaacta
    21961 taaattctat ggtatatgca aagcctaaaa agccacaaga gagtttcttg aaaaaggtat
    22021 ttagatagat gatacctttt actcaagatg taagcatttt ttatgctact atttatggtg
    22081 gaatactcat aggggtgttg tttgattttt atagagggct aagagggaat tttaaattca
    22141 tcaattattt tgctataata tttgatgtat tgttttggtt tctagctacg gttataatat
    22201 tcgtaacaat aaaccttaca gaattttttg atttaagata ttatcatttt gtggctttgt
    22261 ttattggttt tattttatac tataacacca taagtaaaat tgttttatca attatcaata
    22321 aaataattcg ttttgtgaga aattctttta aaaaagttac acattacata gtcagttttt
    22381 taaataattt gtattacgtt ataatctatt cactacacct actatttgat attatatttt
    22441 atataccaaa catatttata gcaacaagaa aaagtataaa aagaaaatca aataaaaaat
    22501 taaagaacaa gaaaaaatca aaacctaaga agaaaaaaaa taaaactaaa aaaagggtgt
    22561 aggaaaaatg aacttaagaa aaaaattttc aggacagatt attgttatca gtttattttt
    22621 aggaatatca atttttagta tgatgacagg atttgtattt gagtatacaa aagctaagga
    22681 atataaaaaa gaaatagcaa gtctaaataa acaattaaaa aagactgaaa tacaaataaa
    22741 cagcttaaag aaagatgaaa aatcatatga aggagactta gaggatatag caagaaaaag
    22801 attgaatatg gtaaaaccaa atgaaacagt ttatgtagac ataaatcgtt gattaaaact
    22861 tgctaataag tttacagatg tattgatttt aaaatttaag atttaataaa aaagtcattc
    22921 agttaaggat ggctttttgt ttttattgag gagggaattt cataatgaaa gtaggagcta
    22981 tagatattgg gacaaactca atgagattat tgatagcaga ttatagagat gggaatttta
    23041 taaacagaaa aaaatttgtt aatacaacta gaattggtta tggtgtggat agtaaaggat
    23101 atataaaaga agatgctatc aaaagaaata taaaagcatt aaaagaattt tctgataagt
    23161 gtaatgatga aaagtgtgaa aaaatttatt gtatggggac atctgctctt agagacagta
    23221 aaaatggaga ttactttgtt aaattagcta aagaagaaac tggaatagat gttgatatag
    23281 ttacgggaat agaagaatca aatttgggct ttaagggtgt tttagaagga atacaaggtg
    23341 atgatactat tattgttata gatattggag gaggttcaac agaatttatt gtaggtgatt
    23401 cagaaggaat taagtttaat aaaagtgaaa atgtaggtgc tcttaggatg acagagaggt
    23461 ttttgaagca cgatcttatt gaagatgaag agtttgaagc tatgagttca tttatatatg
    23521 gtgagattga caaaacgtta aatcatataa aagacagagg attaaaaact cttataggta
    23581 taggaggtac gattacatca ttatcagcaa tgaatcaaga attagagatt tactctatgg
    23641 aaaaaataca taatagtaaa atacacaaaa aagaaataga agaaattcta caaaaattaa
    23701 taaagatgac attaagtgat aaaaagaact taaaagggct acaagaaaaa cgtgcggata
    23761 ttataactgc tggtgtaaaa atattgaata taataatgga aaaattagaa atagaagaaa
    23821 taaccgtaag tgaatacgat aatttggaag gcttaatatg tcaaaagtca aaaaaaatgt
    23881 attaatattt tttgattttt aaaaccgatt atgacaaaaa aaattactcc tctttgttac
    23941 aattcttaat ataaacaaaa gaggaggttt ttttatgcaa agaagtgtag cagttgtgaa
    24001 gaataagact gtaaaaccaa atgtcataaa attaataagc ggatatattg attttactac
    24061 ggcggcattc gctgtgatgg gatttttact tagtaggtct attatagtag actctgtagc
    24121 tcctttagga attgcctttt ttatatgtgc atcaaagatt gataagtaca agatacctgt
    24181 attcatatca acattattag gaatattgtt atcatctaat gattttggaa atgtagcaaa
    24241 atatgctgtg tgccttacaa tatttatggc tatgaataaa aaattaaaat tatcagattc
    24301 aattgctaag gttgcattta taggtgcaat aatactactt ccaatctcca tgggacaagc
    24361 tttcttttca aataggtatg tatatggtct tcttatagca ggagtagaat caataataat
    24421 gtttatatct atatatgtgt tttcttatgg gataggttta atggccaatg tcaacaatag
    24481 gatgtctgta cggacagaag aagccatatc aataagttta ctgctaacct ttagcataat
    24541 gggaatagga gatatatctc tgtttggaat atcaataagg accattttat cgaccgtatt
    24601 gatattgctg acctcaatag taggtggagc taccatggga gcatctagtg gtgtaattat
    24661 tggtgtagca tccattttaa ataatatgac atcagctgtt tatatgggga tatattcttt
    24721 ttcaggatta ataagtggag catttaataa aataaataaa tatttctgta tactaggata
    24781 tattctaagt tggacaataa tatacctata tacttctggt attacttcta atatgatgca
    24841 attaagagat atactattag gttgtttgat agtactagta ttgccagagc gattatttaa
    24901 taagattgaa aaattaataa aaagtaatgt ggcttcaaat gaaatcgtat acgattatat
    24961 tatgagaagc aaaaacctaa ctaatagcag attgaacagt atttataaaa cttatgatga
    25021 ccttgcagat acatttgata agataagaga aaaagacaaa gtgctagacc aaagagacat
    25081 agctaatgta atagatatga ttcataatga tgaatgtaaa agttgtagta tgagaagaat
    25141 gtgctgggag tcaagattta atcatacata cactatgata tataacattc ttgaaaaaat
    25201 tgaggagaaa ggggagttaa gtctaaacga tatacctaaa aattttagaa aggaatgtat
    25261 gaaaccagag tcaatagtga aaatatcaaa tcactattac aaaatgtttg tattagatta
    25321 tgattggagt gtgaagtttt cagaaagtag aaagttaata gcaaatcaga taagaagtat
    25381 ttcaaaatcc attaaatcct tatctcaaga tttagaaggc gacataatgc tagatataga
    25441 aaaggaaaaa aatatatatg aacagttaga aagatatgat ataacagttg ataaagtaag
    25501 ttacttaaca aaaagtaaca gtgagtttga aatatcaata gaaaagaaaa cttgccatga
    25561 tggatgtatg tgtgaagata aaattgtaaa tataatttca gacctagtag gggaaaatat
    25621 gtctgttaga aaaataggat gtcactgttt aggaggtaaa tgcaaggcaa cattcgttaa
    25681 atctcaaaaa tataaggctg ttacagaagt ttcagcaatg tcaagggatg ggcatatcct
    25741 ttgtggagac aattacacat acatggagat aaatgatggt aaatacatga tggctataag
    25801 tgatggtatg ggaaaaggta aaaaagcata tgaagaatct tctgccacta ttgatatact
    25861 ggaaaaaatg atagatgcaa aaattaaaga tgagatagtc attgatacta taaataatat
    25921 gttgttgtta aagtcttcag aagagatgtt ttcaactctg gatttgggaa ttttagattt
    25981 aaaacgaggt tgtttagaaa ctataaagat gggagcgtgt tctacttata taaaaagaga
    26041 ggatggagaa gttgacttaa tatcgtcatc atcattacct gtgggtatat tatcagatgt
    26101 aaaaattgat agaaaaaatg taaaagttaa ggaaggagac tatgttataa tggtttctga
    26161 tggaattgta gatgcaggaa gaaataacaa tttaggagac aattggttaa tatacttctt
    26221 aaagaatata gagactacaa atccaaagga aatttcaaat ttaatattgg atagagcatt
    26281 agagttacaa gcgttacaaa ttgaagatga catgactgtt ttagttacga aaatatgtac
    26341 aaaataaggt ttacaagatt atgtgtataa gatgtggata acttgtgtat aatatgttaa
    26401 tagacttttg aaaaaagtat agttattaac aaaaataaca tataaatcaa caaaatttgt
    26461 tgataagtat ggcagaaatg ttaataaaat gtaaaaaaag aaaaaacaaa agctattatc
    26521 aaaagagatt catgttgaaa taactaaatt tctaagtaaa gaaaacctgt gaatatgttg
    26581 attattatgt ggataactat taattcgata tttatttata tagtatgaat aattgaataa
    26641 tatatctaaa aaaagccatc ctattattaa gtaaacttat tgaaaggatg gctttttatg
    26701 aatagaaaat tgtgcaaagc aataatacct ttatttattt ctattttttg catgttaata
    26761 ataagtataa aagctgacca aggatatcaa aagaataatg acagagcaat tactgtcatg
    26821 aataataatg atactgaaga tgatgctgaa aaaaaacaag aaatagacta tgaaaaaaca
    26881 tgttggaatt tgcaatcttt gtttaaaagt gatgaccaat ggaaaaaaga acttaagagt
    26941 tttgataaag atactaaaga gttgaaaaat tacataggta aagtaacaaa atcgccaaca
    27001 catttgtctt ttgccttaaa cattaaagaa aaacttgata taagactaaa taaactttct
    27061 gcctatgtaa aattaaaaca agatactaat aaaaattcgt ataagtactt agatatgaat
    27121 gatagtatga gtaaatccta tgggaattat atggggattt gttcagattt agagttagaa
    27181 atactaaaat tatcaaacaa agattacaag aaaattattt caaataaaaa tataaatagg
    27241 aaatatggtg catatttaag agatataaga aggaataaag tacattattt ggatgataaa
    27301 tcagaagata ttctaagcaa agtatcttca ataagtagtc ttccaagcca agtgtatgaa
    27361 ttatttagaa atatggatag aaaaactaat ttgacaccag ctcaatatgc gagtgaatta
    27421 gagtcatcag atagagaaaa tagaaaaaaa gcttatcaag atgaacttat aacttacaat
    27481 gataatataa atacaattgc aggtttaatt actggtcaag ttaaaaagaa tattttttat
    27541 tctcaggaaa gaggttatga gtcttcttta gaaatgtacc tggaatctga taatgtagat
    27601 gaaaaagtat acaataattt gataaggact gtaaatagta atatggacag tctacacaaa
    27661 tatatagatt taagaaaaaa agtattaaaa ttagataagg ttcattcata tgatatgttt
    27721 gtgcctatgg ttaaggctgt tgataataat attaattatg aaaaggcaca aagtatagta
    27781 tattcagcgt tgtcaccttt aggaaaagaa tacaatgatg tagtatataa agctttcaat
    27841 gaaaaatgga tagatgttta ttcacatgaa aataaagtaa gtggtggata ctgcttatct
    27901 gtttatgaaa atcatccata tatactttta aattataata attcattagg ttcagtttcg
    27961 actttatcac atgagcttgg acatgctgta tatgaatatt taagttctaa gaatcagaat
    28021 tattttaatt caaatccgtc tatatttact cacgaagttg catctacaac gaatgaagca
    28081 ttactttacg agatgcttat aaaagaagct aaaggcaatg atgaaaaagc ttattatata
    28141 acacagtatt tagacttgat aaaagatact ctttatacac aaactatgta tgctgaattt
    28201 gaaaaaacta tacacgagtt agtagaaaaa ggaaaaagtg taaacgcatt agtattaaat
    28261 gatgtttggg gacaacttct taaaaaatat tatggacaaa gctatgaatt agaccaatta
    28321 tctaaggtcg gatgggctag aatcccacat ttttataata gtttctatgt ctataaatat
    28381 gctacaggtt gtagtgcagg agtaagtttt gcccaagaca tcctaaagaa tggttcagat
    28441 aactatatga gctttcttaa aaagggaagt tcagattatc caataaactt gttaaaatca
    28501 agtggcataa atttaactaa tacaaaaccc atagaaaata caatcaaaaa atttgatagt
    28561 ttagtagcag aacttgaaaa gctattataa acattattgt aatccacatg gtaaatatat
    28621 cgaattaatg ataaaatata ctaaaagttt aaagtcctat caagatagga ctttttattt
    28681 attaattagt gggagatgat tctatgcaag ttaaagttaa aaaggctgta ataccagcag
    28741 caggtttagg aactagattt ttgccagcta caaaggcaca accaaaagag atgttaccaa
    28801 tagttgataa gccaactcta caatacataa tagaagaagc tgtggcctca ggaatagagg
    28861 aaatactaat aataacaggt agaaataaaa aatctattga agaccacttt gataaatctg
    28921 tagaattgga acttgattta gagaaaaaag gtaagaaaga attgttggag gtagtacaaa
    28981 acatatctaa catgattaac atacattata taagacaaaa agagccaaaa gggttgggag
    29041 atgcaattta ttgtgcgagg cattttatag gtgatgagcc gtttgcagta atgttagggg
    29101 atgatattgt agataatgat gtaccttgtc taaaacaact aacagatgca tatgaggaat
    29161 atagaactac aatattgggt gttcaaaagg taaatcaaga agatactaat aaatatggga
    29221 taatagaagc aaaaaatata gaaggtagag tttacaaggt aaaagacatg gtagaaaagc
    29281 cagagtcagg aaaagctcct tcaaatattg ctattttagg tagatatata ataactcctg
    29341 aaatctttga tattcttaag gatttaccac caggtaaagg tggagaggta caacttacag
    29401 atgctttaaa aatattatct aagaaagaag ctatgtatgc atacaatttt gaaggcaaga
    29461 gatatgatgt tggggacaag ctaggatttt tagaagctac tgttgacttt gctctaaaga
    29521 aagaagacct taaggaagat tttataaaat atttgaaaca tgtatgtagt gaatttgata
    29581 aaaataacaa tgtgttatac aataatacag atgaaaaaat agatttaatt gaagtcaatg
    29641 aagctagaga acttgaaatt caaaaataat taatgaaagg tgatgtatgg tggcaaaatc
    29701 aagtcagaaa aaaattaata gaataatagc tgttgggatt gtgggaatcc tcgtaataac
    29761 gagtataaca gctgttttga gtatgattgc aactatgtaa taagataaat gacagcagtt
    29821 gatatacata aatataaaaa ccatatataa cttgatgtag tc
//
LOCUS       DAELLV010000024        29653 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.024, whole genome shotgun sequence.
ACCESSION   DAELLV010000024 DAELLV010000000
VERSION     DAELLV010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29653
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.024"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1029
                     /locus_tag="KPD58_000909"
     CDS             <1..1029
                     /locus_tag="KPD58_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG0264693.1"
                     /translation="FREIDKKYNSNFLEDKEFFNLFYLHIACLIERIKKNHKIINPFS
                     VKISQQYPTVFNLSIQFSKIIENEYHIKISQDEIGFIATHIAVPFEKREEANFNKKYK
                     IAIICSSGGGSAFLINLRLKEIFPNAEIRNFSLLEEKAVIEFAPDLIFSITNLLFEIN
                     APVILINEILDELDYLDIKESVRFADHIGNISSPKQYILGLFDKNHFRCIKDKAKYKD
                     ILDSMSKCIVDEGACSPTYPKDVWERESYLSTIYTNGVAIPHPIEMTGNKNIISVALI
                     QTDIIYENRVPKIIFMISLIKGNLELHKQISKYLSKIMANKDMVDMLNKSQSYEEFMY
                     KLKIYLGG"
     gene            1032..2075
                     /gene="ypdE"
                     /locus_tag="KPD58_000910"
     CDS             1032..2075
                     /gene="ypdE"
                     /locus_tag="KPD58_000910"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBG0264694.1"
                     /translation="MNIEFFKRLSEADGIASNEEEVRHVLLEELKAYSDKIICDGLGS
                     IIFSKIKDESAPNVMICAHMDEVGFMVRSIDKLGMIHLITIGGVKPLAQFVQKVRITT
                     KEGKKIPAVINATYNNGKAENIYADIGAYTEEDVYNLGINVGDMVTYTTSFEEFTLPD
                     RLVGKAFDDRIGCFVMGEVLKELRKENLNCNIHFAATSSEEVGIRGAKTSTQLINPDI
                     VFVIDVACAKNEFVRDYTNKKQIDKGVMLMHRDRTLVPSRKMIDYVMEIADKNNIPLQ
                     HDMFESGGTDGGEAHLVNEGKPCVVTCVPVRYGHCAFSIVSNKDLENIIRLYTQLILN
                     FDEEQYKHIRNFI"
     gene            2090..2395
                     /locus_tag="KPD58_000911"
     CDS             2090..2395
                     /locus_tag="KPD58_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG0264695.1"
                     /translation="MSTEVQIMELISTAGESKSKAFEALKKVKSKDFKGAKLLIEEAR
                     KIDIEAHRIQTELITQDLNEDVESPAMSLLMVHAQDHYMTSQLAKDLIEALIYCLDK"
     gene            2492..2827
                     /locus_tag="KPD58_000912"
     CDS             2492..2827
                     /locus_tag="KPD58_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0264696.1"
                     /translation="MKILLCCAGGFSTNMLMQNMKKVVKESAKLNEKDFEFTAIPADS
                     IESEVDDWDIVLIGPQIAHKIDFLKGILEPKNKPFAVIDKDVYGKMDGATVMKLALIT
                     YKKHQLAQG"
     gene            2859..4181
                     /locus_tag="KPD58_000913"
     CDS             2859..4181
                     /locus_tag="KPD58_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0264697.1"
                     /translation="MFSKFEAFMNRVFMPLAHKVDNQRHLGSIKAGMVAMTPFTILGS
                     IFAILPALPNMLGENNPISKFILSNSAILDLPVSLSIGLIGLYACMCIAFNLGNYYKL
                     YIPGCVALSTFSFLFLVASFTPDGALEIGNLGAKGLFTGMIAGLGSVELYNFCNKKNI
                     TIKMPENVPDFVSRSFELIPTTIIVCGTFIVARFISLKVFGELPPQILTQFLSPLVGS
                     MDNPWSVLALNFAICTIFFFGIHSSVFSPITRPIMITFIAENIAAMQAGETIPHFFTA
                     GASSAFFGFTGCGISIGCVIACMLSKNASYKKIGRVSLFPALFGINEPILFGAPIILN
                     PIMFIPHVFGGAIIGTLPMFFMHWGWLAKPVFDPPYVGVFLEGFLTNGDWHSILANAL
                     QLVLAVLLYWPFFKIMERQQNNKKQKEETKNIFSKEEEDLLDDLGLDF"
     gene            4191..5261
                     /locus_tag="KPD58_000914"
     CDS             4191..5261
                     /locus_tag="KPD58_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG0264698.1"
                     /translation="MNNTMERLVSLIYKRGVDALLIKAKNTKRYIGALSGSGVYVLVT
                     KDKNYQILDGRYIDEADKKTTGFIKRIVSQGSYIPTIIELLKELNIKKLAIENQAMSI
                     QEYLSLKNEGFEINLITNELWKLRAIKSKEEIKLIKKACEITDEVFDEVISEIKKDMT
                     ELEVSALIQYHALKKGASGMSFETIVVSGERGAMPHGRPTNKKLKVNEAITIDFGVVY
                     QGYQSDMTRTISIGKPPKIIKEIYDVVLEAQLSAIESIKEGTRASDVDKVARKIIDKH
                     GFGEYFNHGLGHGIGLGDGEVPTLNPNSEDILVEGMVMSCEPGIYIPNVGGVRIEDDI
                     VIIDGKGVPLNKTSKEFIILGE"
     gene            5266..6453
                     /locus_tag="KPD58_000915"
     CDS             5266..6453
                     /locus_tag="KPD58_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG0264699.1"
                     /translation="MKYNFNKVLNRKEGNCRKWSNHVIKEKFGLNEDAIPMDLADIDF
                     ECAPAIKESILKRASVGDYSYTFIGDDFYDAIINWNKRRFDVYIEKDWIKLTFGTVST
                     LHYIVQAYTKEGEGVLINTPAYDPFAEAVVNNNRKLCCSSLKLDNNRYYLDLEDMENQ
                     MKYENIRLLIFCSPQNPSGRIWTKEELYQVSELCLKYNVILVSDEIHRDIIFKDYRFV
                     SLWNAHPEIYKNSIICVSPNKGFNLGGLKTSYVLIRDVKIRETLLERLKSNSITSPNV
                     FAIPAIVAAYNESEEWLDAMTSYVEENFEIVYDFFETNIPKAKVMKSDSSFLAWIDVR
                     EVFRNEEESKEFFRHANLTMVVGSYFVKDGDGFIRINVGCPRETLNEALNRIKKTYIS
                     MYC"
     gene            complement(6650..7522)
                     /locus_tag="KPD58_000916"
     CDS             complement(6650..7522)
                     /locus_tag="KPD58_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBG0264700.1"
                     /translation="MNILQNENLIIESSSFGAELTKIFSKKYDKNILWDGNKKYWGRQ
                     SPILFPIVGKLFDNETIIEENLYSMTQHGFARDMDFEIVDKGDTFVTYKLTDNESTLK
                     KYPYSFELIINYTLNDNSIEVEWIVKNTDSKDIFFSIGGHPAFNLPFYEQNDFSKHYL
                     EFKSKNDVEKINLNGSFTDDIIPIGKLKNLQLNPEVFKNDALIYTNIDEVSICNNDGS
                     KYVTISMEDFPLVGIWTPYYSETNSTAPFLCIEPWYGLADSINSNKIYKDKKFINKLS
                     KGKVFTASYNINIH"
     gene            complement(7916..8107)
                     /locus_tag="KPD58_000917"
     CDS             complement(7916..8107)
                     /locus_tag="KPD58_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264701.1"
                     /translation="MDDFSKLKSLYEDGYRCIYHDCVDNNYTIYLKNFYTEGSETIEL
                     SSESDFSQFKDYIDGLRMS"
     gene            8327..9373
                     /gene="cobT"
                     /locus_tag="KPD58_000918"
     CDS             8327..9373
                     /gene="cobT"
                     /locus_tag="KPD58_000918"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HBG0264702.1"
                     /translation="MSLLESISKNIYSLDNSSIEKTKQRLDRLIHPTGSLGKIEDICM
                     QLAGIFGNENFDTSKKVIIAFAGDHGVYEEGVAPDPQNITKLQFPNFSKGLCGVGVIS
                     KFVGADVVAVDVGINCDEKLDGVLDYKIRKGTSNMAKGPAMSKQEAIRCLEIGIEIAE
                     QCIEKDYKVIGIGEMGIANTTPSTAIISVISGCDPLEVTGIGAGLKKERLKHKAEVIR
                     KAIEINNPNPTDGVDILSKVGGFEIGSMAGVILGCSANRIPVVIDGFISYAAALIAYK
                     INPKTREYMIASHLSAESGTKRALDILKLDPLLNMDMRLGEGSGAALAFDIIEASNYT
                     YKNMATFDEIDMGR"
     gene            9405..9965
                     /gene="cobU"
                     /locus_tag="KPD58_000919"
     CDS             9405..9965
                     /gene="cobU"
                     /locus_tag="KPD58_000919"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HBG0264703.1"
                     /translation="MSKIILVTGGARSGKSNFAEKLCFDRNNNTAYIATSIPFDDEMK
                     DRVKKHQENRPRNWNTYEIYEDIYSIIKEIYKEHETVILDCVTLLVNNLMFEHNLNIE
                     ESSQEEINKLEQYIKEQVVKLIEEIEKTNLYFVFVTNELGMGIVPGNKLSRIYADIVG
                     RINQYIASKSNEVYFVVSGIAMKIKE"
     gene            9975..10748
                     /gene="cobS"
                     /locus_tag="KPD58_000920"
     CDS             9975..10748
                     /gene="cobS"
                     /locus_tag="KPD58_000920"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HBG0264704.1"
                     /translation="MKRFILILQFLTRIPIKLNVGFDDEFYKSIVYFPLVGFVIGILS
                     YLIGWISMLLFEPFIASIIITLAGVLITGGLHIDGLGDTFDAIYSYRDKEKMLEIMKD
                     SRLGTNSLLAIMFVLLLKVGFVYDIISNNSLWVIIFMPMIARLGVMLLTYKTVTPREK
                     GMGNLFIGKLTTSMLITAIIYTLLIVALITKFIFLLPNIVLIKVLGSIIVVFVFIILF
                     KKHIYKKIDGVTGDILGCGIELSELVYLIYIYLLIFMFF"
     gene            10768..11382
                     /locus_tag="KPD58_000921"
     CDS             10768..11382
                     /locus_tag="KPD58_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBG0264705.1"
                     /translation="MIRLILIRHALTNDNKKGRLSGHINSCISEEGKLQINKITRYLS
                     NENIDKVYTTPSTRTKDTVEKISKLKLLEIEEKEALREISFGDFEGRTFEEIKIKYPN
                     EFEKMIKEGNNYRYPNGESLIDSYKRVAKEIDNIILENNSNLDTKTILICSHAGTIRN
                     IITHLISGSYKYHWNFKIDNASITVLEIDGGFAVIDKMNFTDFI"
     regulatory      11449..11628
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            11758..13257
                     /locus_tag="KPD58_000922"
     CDS             11758..13257
                     /locus_tag="KPD58_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HBG0264706.1"
                     /translation="MLQGTASNVGKSVLVAGLCRIFKQDGYTVAPFKSQNMALNSFIT
                     KEGLEMGRAQVFQAEAAKIEPIADMNPVLLKPSGNNKSQVIVRGKVVGEMPSSKYHDY
                     KLELVDVLKETFNSLNSRYDIVVMEGAGSNAEINLKDRDISNMGMAEIADAPVIIVGD
                     IDRGGVFASLAGTMLLLNEDEKRRVKGVIINKFRGKKELLQDGVKMLEDIIKVPVIGV
                     VPYTDIKIEDEDSVTTRFKKKMNKSDIHIEIIRTPHMSNFTDFNIFETQEDVSIRYVD
                     YGESLGNPDILIIPGTKSTIDDLTYIRKSGLESQIKNLHSQGKLIFGICGGYQMLGKK
                     LKDPYHVESEIEEVDGIGLLDTETTFEKEKTTTQVEATIVECISEYMNNLKGKKVKGY
                     EIHMGITKRGNDVRSLDLITKKLDKEVNYTEGSVNKEGNVIGTYLHGIFDEISFTRAI
                     LNNIRKKKGLGELESKVNSFNEFKDKEYDKLADFLREHLDIEKIYEIMK"
     gene            13296..14672
                     /locus_tag="KPD58_000923"
     CDS             13296..14672
                     /locus_tag="KPD58_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HBG0264707.1"
                     /translation="MKKILIAGTNSGVGKTTISLGIMQALVKRNMKVQPYKVGPDYID
                     PSYHTFITGRHSRNLDSYMLDDEKIKYIVNKSSKDADISVIEGVMGLYDGFGIDLDNC
                     TSSHTSKVLKSPVILVINGKSMAASSAAMVLGYKELDKDVNIKGVIVNNVKTNSHYQI
                     IKSSIEKYCNVEVLGYFPPNNKFSLESRHLGLVPSVEMKSLREKFYTLADEIEHYIDI
                     DRIIEISESDEFESDFDFQTLIENNKIKEHVNNKSIAIAYDKAFNFYYRENIELLEEL
                     GLEINYFSPLEDASVPNADYIYIGGGFPEIFGKELEANESMRTSILEAHNNNIPIYAE
                     CGGLMYLGEKLLNQEEQEFNMVGIFSGISKMTSSLKRFGYCIGVAKENTLLSKKGESI
                     KGHEFHHSIFESDEKCVYSMRKERDGNIVEEWDGGYSKGNTLATYLHTHFYNNLNCIE
                     NFLKKGNE"
     gene            14673..15644
                     /locus_tag="KPD58_000924"
     CDS             14673..15644
                     /locus_tag="KPD58_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBG0264708.1"
                     /translation="MSILSIYIGYVTDLIVGDPYSFPHPVRFIGKLINFTQCIIRKVS
                     KNDRQLKFGGFILWLITVGITYLTTYAIVKLFSFNILLSIVVNSFIIYTTLATKCLKD
                     EALKIYNVLKTEDIEKSRIQLSYIVGRDTTNLSEPEIIRATVETVAENTVDGIIAPMF
                     YAFIGGAPLAMAYKAINTLDSTVGYKNKKYKDIGFASAKIDDIANYIPARISVILMTI
                     GSFFLNYNYRNCFKISIRDRKNHKSPNCAFSEGAVSGALGIQLGGTNVYFGEKVYKPT
                     IGDKLREIDKEDIIKTNKIMYASSFVSMLVFTLIYVLCRMILINLII"
     gene            15676..16746
                     /locus_tag="KPD58_000925"
     CDS             15676..16746
                     /locus_tag="KPD58_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG0264709.1"
                     /translation="MKDLGHGANVDEMARLYGKNPKEIIDFSSNINPNVLPNLERYIL
                     KGLEECRNYPDINYTNLRENISKYIDINPDFIIPGNGATEVIYLLMKSIKKKLAIINP
                     TFSEYRRSAELNNLDIIDLELDLENNFKLNIDIIKENIKRFDSLFICNPNNPSGNVQD
                     LKELVHLLDKHNKVLIIDETFMEFVEDESKYSLVKYIESNKNIFIIKAVTKFFGMPGL
                     RLGYGLTSNTEIMNKIYEHKEPWTINSFADILSNFIFEDKEYIKNSKEYYIEERKYML
                     QELRNIRNIKVYDTDANFILIRIYKKTTKELKKDLFKQGNILVRDASNFIGLDDSFIR
                     VAIKSHEDNKILIENMKNLLGD"
     gene            16748..17635
                     /locus_tag="KPD58_000926"
     CDS             16748..17635
                     /locus_tag="KPD58_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBG0264710.1"
                     /translation="MKSYGICPASCGEFVQGIIDDEEYLCSYAIDMYSKVYIEEKLVD
                     INLGRYKSRLAIEKVFEKFNLPKKYTKNISLNINSKIPVGKGMASSTADIGATIKATL
                     SLIDKDLSSEEISKLAAEIEPTDSIFIDKNSIFNPLNGTVIKYLGNLTNAKVVILEPN
                     KVLDTMKIRLRQDYNKLKVENKEVIKKSFALLEEGLKKNNLSLVGEACTLSSLANENI
                     EKKECLNEIIKISKKYGAYGVNIAHSGTVVGILIDKSMNDKKMIDALCESNINSVYNK
                     IYTQNIINGGIKGEIEWNT"
     gene            17623..18255
                     /locus_tag="KPD58_000927"
     CDS             17623..18255
                     /locus_tag="KPD58_000927"
                     /EC_number="5.4.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003724727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-8 methylmutase"
                     /protein_id="HBG0264711.1"
                     /translation="MEYIKNPMKIEEKSFELIQEIIDEIRPDYKFKNNIEEKIIKRAI
                     HTTADFDYLDILKISEEAVDSIIDALKNNASIYTDTNMALSGINKRKLESLGCKYKCL
                     VADDETVKLAKEKGITRSMAAVEIAAKEEGRKIFVLGNAPTALYKVMEMKSEGRLDLD
                     AVIGVPVGFVGAQESKDEVEKTDIPYIISKGRKGGSNLAAAIVNAILYTM"
     gene            18264..19490
                     /locus_tag="KPD58_000928"
     CDS             18264..19490
                     /locus_tag="KPD58_000928"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HBG0264712.1"
                     /translation="MEEYVYIDGKKYRRGYTTGSCATGASKAAVYMLITKNRINTINI
                     DTPKGIPLLLKVDNINISDTFVECSIKKDGGDDIDATHTMDIYARAEIVAKNDKNKGY
                     LTLKDIDSLSTNSECKSELYKFIRVYGGTGIGVVTKKGLSVDVGKPAINPTPLKMINH
                     EIRKLIGDNFESILGNDKVLKITIFAPQGETVAKKTFNPRLGIVGGISIIGTTGIVEP
                     MSDEGWKKSLSIELQMKKEQGLDKIILVPGNHGEQFIREKLNLDIKYVVRVSNFIGYM
                     IKEAQRIGYKKILMAGHIGKFIKVSAGIFNTHSKVADARSEILVANLALMGARYEFLN
                     KINQCVTTEEAVELINNSEYREVYNILSNKCRERVKQYLNEDSDDIDVEVIIFSMDKS
                     LLGKSDNTDDLVEVFI"
     gene            19487..20095
                     /locus_tag="KPD58_000929"
     CDS             19487..20095
                     /locus_tag="KPD58_000929"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HBG0264713.1"
                     /translation="MINIIGLGPGNLDYITKKGENLISTSDVLIGGKRNLESIKNFEG
                     EKIVLDSNLREIIEYINNNKEKQISIIASGDPLIYGIGRYLSKNIDNKMLNMVSGISS
                     LQYIFSKIYVDMNDVYITSSHGKVPDFDYILSHKKVCMVTDSKIGPKQISREIIDRNL
                     NKIIVVGENLSYDNEKITIAKPEEIIRIDNFDMNVVVILDEE"
     gene            20085..20660
                     /locus_tag="KPD58_000930"
     CDS             20085..20660
                     /locus_tag="KPD58_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating cobalt-precorrin-6B
                     (C(15))-methyltransferase"
                     /protein_id="HBG0264714.1"
                     /translation="MRNSEFITGKVPITKEEVRAISISKLNLVNAKNLIDIGAGTGSV
                     SVEAAYNYPNLKVISIEKNDDAIELIEKNIKKFNLKNIEVIKGCAPINLGLNVKVESI
                     FLGGTGNNLEEIIEWSKKNLVTGGRLVANFIIIDTFNQTLKLLKKHGFKEIDVCVLNV
                     SKLEKLGKGEYFKPLNPIYIISCEKGENDDE"
     gene            20653..21405
                     /locus_tag="KPD58_000931"
     CDS             20653..21405
                     /locus_tag="KPD58_000931"
                     /EC_number="2.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4 methyltransferase"
                     /protein_id="HBG0264715.1"
                     /translation="MNKVHFVGAGPGDKELITLKGYKLLSNADVVIYAGSLVNPELLE
                     YCKEDCQIHNSAHMDLQEIIDVMREGIENNKSVVRLQTGDFSIYGSIREQVEDLNKLN
                     IDYDCTPGVSSFLGAASSLGVEYTVPEISQSVIITRMEGRTPVPEKESIQSYAKHQTS
                     MVIFLSVQEIEKVVSKLLEGGYPKDTPIAVIYKATWADEKIVKGTLSDIAVKVKENNI
                     NKTALIMVGRFLGEEYNNSKLYDKDFKHEYRG"
     gene            21389..22510
                     /gene="cbiG"
                     /locus_tag="KPD58_000932"
     CDS             21389..22510
                     /gene="cbiG"
                     /locus_tag="KPD58_000932"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HBG0264716.1"
                     /translation="MNIEGNIAIICITENGKKLALKINSLVNNCVIYQVKNKKSNLVV
                     EETCVHTVQKKLSDFVGEIFSKFDYIVFIMATGIVVRSIAPYIISKFSDPAILVMDEK
                     GKNVISLLSGHIGGANSLTQYISSLIDANPVITTATDINQKASLDMIAKKLNAHIDNF
                     RESVKEVNSMLVNNKCVGLYVDEGYQVDTRGFKLIESKDELRDSFSNTYIEKLVVVSN
                     KINCIEDIENLNLDNKFEHCYSTQKIIKVIPRDIVIGIGCRRNTDSELMYNSLEEFLN
                     KQNIDIRAINTVGSIELKKDEKAIIELSKILKVPFKTFSVDEISKVDNLFDKSDFVKK
                     NVGVYSVAEPVAYLLSKGNLLVEKHKYKGITFSVGRIKI"
     gene            22507..23232
                     /gene="cobJ"
                     /locus_tag="KPD58_000933"
     CDS             22507..23232
                     /gene="cobJ"
                     /locus_tag="KPD58_000933"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011701973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HBG0264717.1"
                     /translation="MIYVVGIGPGSKDTMTLEAIKAIEDSEAIVGYKTYIKLIEEFIQ
                     GKEIIQNGMRQEVDRCKQAVEEAKKGKKVAVVSSGDAGIYGMAGLILELISKEDEDIK
                     VKVIPGVTASIGAAAILGAPIMHDFCHISLSDLMTPWEVIEKRLRLAAEADFVICLYN
                     PRSKGRSEHLSKAFKIMGEFKGGDTPVGVVKDVGREKEEKFVCTFENMDFEKVDMTTM
                     VIIGNKSTYINGELMITPRGYTV"
     gene            23229..23981
                     /gene="cobK"
                     /locus_tag="KPD58_000934"
     CDS             23229..23981
                     /gene="cobK"
                     /locus_tag="KPD58_000934"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HBG0264718.1"
                     /translation="MILILGGTSDSLSICDKINELKNQPYILSVTTDYGRELAQQHAE
                     NVILGKLGKEDMLKFIKDNNIVKIIDATHPYAVEVSKTAITCAKLLNIDYIRFERKSL
                     IEEIDYDNKFIVDTIEEACEIANKIGTNIFIGTGSKNLNRFIEEISDKNLVVRVLPTS
                     EVILSCEKLGLSADNIVAMKGPFSQYINEETYKHYNIDLVITKESGIAGGFLEKVNAC
                     KTFNIPVIIIKRESINYENVFNDINKIKDILV"
     gene            24000..25478
                     /locus_tag="KPD58_000935"
     CDS             24000..25478
                     /locus_tag="KPD58_000935"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HBG0264719.1"
                     /translation="MKKALLVVSFGTSYHETREKTIDICEDKIKNSLKSHDFFRAYTS
                     NMIINKIKKRDGIEIDNPIQALDKIYEQGYDEVIIQTLHIICGEEFNKLKEQVDGYSS
                     KFKKIVLGRPLLTHIEDYQEAVEAIKHQIPHMESNEAVVFMGHGTLHESHSAYPALEY
                     MLRDNGINAYVGTVEGYPEIEHVIRRLKEGNIKTVNLMPFMLVAGDHAINDMAGDEED
                     SWKTILEENGFNVRVHLKGLGENSYIQDKFVRHAVKCAENAKFYGIGAGPGDGSLLTI
                     KAVNTLKKLDILYTPESKKGGDSLALSIVSEYLPESVEIKSRHFPMSFDGNEKSLAWD
                     NVAQEIISDVNDGKNVGFVTLGDPMIYSTYVYIMKRILGEVEVETIPGISSFSNIASN
                     QNFPLVMDKEALVVIPCTMEDDKIEYALQNYNSIVLMKVYKNFKEIIKKLEKYDLIEH
                     AILVSNSSQESENVFTDLKEAHLEDKISYFSTILVNKDNKIK"
     gene            25508..26413
                     /gene="hemC"
                     /locus_tag="KPD58_000936"
     CDS             25508..26413
                     /gene="hemC"
                     /locus_tag="KPD58_000936"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HBG0264720.1"
                     /translation="MNIVVGTRGSNLALIQTEWVINELKKKYPEISFEIKIIKTKGDL
                     IQNVSLDKIGDKGLFVKEIEQQLLDGKIDIAVHSMKDMPSYLADGLKFAHTPKREDPR
                     DVLILREGYKNLDDLPHGAVIGTGSKRRKFQLLKQRPDLNIVQVRGNVETRIRKIKDE
                     NMHGIVLAASGIIRANLQDKISSYLPVDVVIPAPAQGALAIEIRSNDSTIEGIVNSLK
                     DENTEIQILAERGFLDGVNGSCHIPIAAYCEIIQDKIHLTGLYGDSEGKKVVIKSIDG
                     DISSPRELGLKLAKLVLKEYENYEG"
     gene            26403..27902
                     /gene="cobA"
                     /locus_tag="KPD58_000937"
     CDS             26403..27902
                     /gene="cobA"
                     /locus_tag="KPD58_000937"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBG0264721.1"
                     /translation="MKGKVYLVGAGPGDYKLMTLKGLECIRKSDVIVYDRLANSSYLR
                     EAKPDCELIYVGKASSNHILPQEDINRIIAEKAKEGKIVTRLKGGDPYVFGRGGEEAE
                     TLVDEGIEFEVVPGITSAIGGLCYAGIPITHRDHASSFHVITGHLKGDDSGELNWNAL
                     ANNKGTLVFLMGISNLEKISENLMKEGKDKDTPVALISWATRYNQRVVTSTLENVYET
                     AIKEEVKPPTLIVVGSVVGLREKLNFFESKPLFGKSIAVTRSRNQNSVLVEKIMDLGG
                     NPIEIPTIKVEKIQNNINLENEIKNINKYNYLILTSKNAVEIFFEKIYEMNFDLRILS
                     NLKVCAIGSATSNELKKRGIIADIVPKKFVAESLYEELAPILNDDDNILIPRAENARD
                     YLVDKLKAICEVTEVHIYRTVIDEEKKGEILDILNSNDIDYITFTSSSTVNNLVEIIG
                     KENIKILEKIKTLSIGPITTETINSLGIKLYKESATSTIDSLVDLIIKN"
     gene            27919..28884
                     /gene="hemB"
                     /locus_tag="KPD58_000938"
     CDS             27919..28884
                     /gene="hemB"
                     /locus_tag="KPD58_000938"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HBG0264722.1"
                     /translation="MLRRPRRLRASKAIRNLVRETKVNVEDLIYPLFVVEGENIKEEI
                     KSLPDVYHLSIDMLEDEIKEISELGIEHIMLFGVPDEKDECASQAFNDNGIVQKAIRK
                     VKEINSDINVITDVCMCQYTSHGHCGILTDTGYVDNDKSLEYLAKISVSHAKAGADMV
                     APSDMMDGRIQAIREALDENGLENVGIMAYSVKYASNYYGPFREAAHSAPSFGDRKTY
                     QMDPANSNEAIIETELDILEGADILMVKPALSYLDIIRRVKDNFDLPLAAYNVSGEYS
                     MLKFAVKEGLLNEDVILESITSIKRAGADIIITYFAKDIAKLLKK"
     gene            29037..29615
                     /locus_tag="KPD58_000939"
     CDS             29037..29615
                     /locus_tag="KPD58_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HBG0264723.1"
                     /translation="MLYPINLKLDELDVVIIGGGEVAYRKCKNFLEFNKNVTIVSKQI
                     LNKFYDLKGNIKIIKDDYKEIYIKDASVIIAATNNRELNMEIGLYCRAKNKLVNVVDN
                     IEISNFTVPSYIKRGDLLISVSTGGKSPSLSSKIKKEIEERYTEDYEEYLNVLGDIRK
                     KVIKKYEDKSKRKDVLNMLVTLDLEELKKFDI"
BASE COUNT        11952 a         2979 c         5511 g         9211 t
ORIGIN      
        1 tttagagaga tagataaaaa atataactct aattttttag aagataaaga gttttttaac
       61 ttattttact tacatattgc atgtttaata gaacgtataa agaagaacca caagataata
      121 aatccttttt cagttaaaat aagtcaacag tatccaactg tttttaattt atctattcaa
      181 ttttcaaaaa tcatagaaaa tgagtatcat ataaaaataa gtcaagatga aatagggttt
      241 atagctactc acattgctgt tccttttgaa aagagagaag aagctaattt taataaaaaa
      301 tataaaatag caataatatg ctcttctggt ggaggtagtg catttttgat aaatttaaga
      361 cttaaagaaa tttttccaaa tgctgagata agaaattttt cattactaga agaaaaagct
      421 gtaatagaat ttgcaccaga tttgattttt tcaataacaa atcttttatt tgaaataaat
      481 gctccagtta tacttataaa tgaaatttta gacgaattgg attatttaga tattaaagaa
      541 agtgttagat ttgcagacca tataggaaat atatcaagcc caaaacaata tatattaggt
      601 ctatttgata agaatcactt taggtgtata aaagataaag ctaagtataa ggatatcctt
      661 gatagtatgt caaaatgtat agttgacgag ggagcttgtt ctccaaccta tcccaaagat
      721 gtatgggaaa gagaaagtta tttgagtact atttatacaa atggggtggc tatacctcat
      781 ccaatcgaga tgacaggaaa caaaaatata atatcagtag ctttgataca aactgatatt
      841 atttatgaaa atagggtgcc taaaattatt ttcatgattt cattaataaa gggaaattta
      901 gaacttcata agcaaatatc taaatattta agtaagatta tggcaaataa ggatatggta
      961 gacatgctaa ataagtcaca atcttatgaa gagtttatgt ataagttaaa aatctatctg
     1021 ggaggttaga catgaatata gagtttttta agaggttgtc agaagcagat ggtatagcat
     1081 ctaatgaaga agaggttaga catgttttat tggaagaact aaaagcttat agtgataaaa
     1141 ttatctgtga tgggctaggg agtatcatct tttctaaaat aaaagatgag agtgcaccaa
     1201 acgtaatgat ttgtgctcat atggatgaag ttggatttat ggtgagaagt atagacaaac
     1261 ttggtatgat tcacttaata acaataggag gagtaaaacc tcttgcacag tttgtacaaa
     1321 aagttagaat tacaacaaaa gaagggaaga aaatccccgc tgtcataaat gctacatata
     1381 ataatggtaa ggctgaaaat atatatgcag atattggagc atatacagaa gaagatgtat
     1441 ataacctagg gattaatgtt ggggatatgg taacatatac aacaagcttt gaggaattta
     1501 ctttaccaga ccgattagtg ggaaaggctt ttgatgatag gataggatgt tttgtgatgg
     1561 gagaagtttt aaaagaactt aggaaagaaa atctaaattg taatattcac tttgcggcta
     1621 caagtagtga agaggttgga ataaggggag caaagacatc aacacagtta ataaatccag
     1681 atatagtatt tgtaatagat gtagcatgtg ctaaaaatga gtttgtgaga gactatacaa
     1741 ataagaaaca aattgataaa ggtgtaatgc taatgcatag agataggact ctagtcccta
     1801 gtagaaaaat gatagattat gtaatggaaa ttgctgataa aaataatata ccacttcaac
     1861 atgatatgtt tgaatcaggt gggactgatg gaggagaagc acatctcgta aatgaaggga
     1921 aaccttgtgt agtaacttgt gtaccagtta gatatggtca ttgtgcattt tcaatagtaa
     1981 gtaataaaga tttggagaac attataagat tgtatacaca gttaatttta aattttgatg
     2041 aagaacaata taaacatata agaaatttta tctaaaggag aataagaaaa tgtcaacaga
     2101 agtacaaata atggaactta tatcaactgc aggagagagt aaatcgaaag cttttgaggc
     2161 tttaaaaaaa gtgaagtcaa aagattttaa aggagcaaaa ttgttaatag aggaggctcg
     2221 taaaatagat attgaagctc acaggataca aacagaatta ataactcaag atttaaatga
     2281 agatgttgaa agtccagcaa tgagtctttt aatggtacat gctcaagacc actatatgac
     2341 ttctcagcta gcaaaagact tgatagaggc tttgatatat tgtcttgata agtaattgta
     2401 atatagaaat agattattta tgtaaaaatt cttttaaaag tttagatgct tttaagagat
     2461 aataaaaata caaactttag gaggaattat tatgaaaata ttattatgtt gtgcaggtgg
     2521 attttctact aatatgttaa tgcaaaatat gaaaaaggtg gtaaaagaga gtgctaaact
     2581 caatgaaaaa gattttgagt ttacagctat accagcagat tcaatagaaa gtgaagttga
     2641 tgattgggat atagtactta taggtcctca aatagctcac aaaatagact tcttaaaagg
     2701 aatattagag ccaaaaaata agccttttgc agtaatagat aaagatgtat atggaaaaat
     2761 ggatggagct acagttatga agttggcatt aataacttat aagaaacatc aattagctca
     2821 aggataataa aaataatgag ggaaaaggga gattagatat gttttcaaaa tttgaagcat
     2881 ttatgaatag agtatttatg cctttagctc ataaggttga caatcaacgt caccttggtt
     2941 ctataaaggc tggtatggtt gctatgacac cattcacaat attaggaagt atatttgcaa
     3001 tacttccagc acttccaaat atgcttggag aaaataatcc aataagcaaa ttcatactta
     3061 gcaattcagc aatattggat ttaccagtaa gtctttccat aggtttaata ggattatatg
     3121 cttgtatgtg tattgctttt aatctgggga actactataa attatatata ccaggttgtg
     3181 ttgcactatc aactttttct tttttattcc tagtagcctc atttacacca gatggagctt
     3241 tagaaatagg aaatttagga gctaaagggc tatttacagg tatgatagca gggcttggtt
     3301 ctgtggagtt atataatttt tgtaataaga agaatatcac aataaaaatg ccagaaaatg
     3361 tacctgattt tgtatctagg tcttttgaat taatacctac tactataata gtttgtggaa
     3421 cttttatagt tgctcgtttt atttcattaa aagtatttgg agaattacct ccacagatac
     3481 ttactcaatt tttatctcca ttagtaggaa gtatggataa cccatggtca gttcttgcac
     3541 ttaactttgc tatatgtact atattctttt ttggaataca ttcatcagta tttagtccta
     3601 taactagacc tataatgatt acatttatag ctgaaaacat agcagctatg caggctggag
     3661 aaacaatacc tcatttcttt acagcaggag cttcaagtgc attctttgga tttacaggat
     3721 gtggaattag tataggttgt gtaattgcat gtatgttatc aaaaaatgct tcctataaga
     3781 agattggacg tgtatcatta ttccctgcat tatttgggat aaatgaacca atattatttg
     3841 gagcaccgat aatattaaat ccaataatgt ttataccaca tgtatttggt ggagctataa
     3901 taggtacact accaatgttt tttatgcact ggggatggtt agcaaagcca gtatttgacc
     3961 ctccatatgt aggggtattc ttagaaggat ttttaactaa tggagattgg cattctatat
     4021 tagcaaatgc attgcaatta gtattagcag ttttattata ttggccattc tttaaaataa
     4081 tggaacgtca acaaaataat aaaaaacaaa aagaagaaac taagaatata ttctctaaag
     4141 aagaagaaga cttattggat gatttaggat tggatttcta atattttgtt atgaataata
     4201 caatggagag attagtatcc ttaatctata aaagaggtgt tgacgctctt ttgattaaag
     4261 caaaaaatac taaaagatat ataggagctt tatcaggtag tggtgtatat gtgcttgtta
     4321 ctaaggataa aaattatcaa attctagatg gcagatatat tgatgaagca gataaaaaaa
     4381 cgactggttt cataaaaagg attgtatctc aaggttctta tataccaact ataattgagt
     4441 tattaaaaga attaaatata aaaaagttag caatagaaaa ccaagctatg agtatacaag
     4501 agtatctttc actaaaaaat gaaggttttg aaatcaattt aataacaaat gaattatgga
     4561 agctaagggc tataaaatct aaagaagaaa ttaaacttat taaaaaggct tgtgagatta
     4621 cagatgaagt atttgatgag gttatttctg aaattaaaaa agatatgact gagctagaag
     4681 tatcagctct aattcagtat catgcattaa aaaaaggtgc aagtggaatg tcttttgaaa
     4741 caatagtggt atcaggagaa agaggtgcaa tgccacatgg aagacctaca aacaaaaaat
     4801 tgaaagtgaa tgaagctata acaatagatt ttggtgttgt ttatcaaggg tatcaatcag
     4861 acatgactcg tacaatatct ataggcaaac ctcctaaaat tattaaagag atatatgatg
     4921 tagtattgga agcacaatta tctgcaatag agtcaataaa ggaaggaact agggcaagtg
     4981 atgttgacaa agtggctaga aaaattatag ataaacatgg atttggcgaa tattttaatc
     5041 atggactggg tcatggtata ggtcttggag atggtgaagt tcccacttta aatccaaata
     5101 gtgaagatat attagtggaa ggaatggtaa tgtcttgtga gccaggtatt tatattccaa
     5161 atgtaggggg agttagaata gaagatgata ttgttataat agatggaaaa ggtgttccac
     5221 tcaataagac atccaaagag tttataatat taggagagta aaattatgaa atataatttt
     5281 aataaagtat taaatcgtaa agaaggaaat tgtagaaaat ggagcaacca tgtgattaaa
     5341 gaaaaatttg gcttaaatga agatgcaatt cctatggatt tagctgatat cgattttgag
     5401 tgtgctcctg ctataaaaga atctatctta aaaagagcat cagtaggaga ttatagttat
     5461 acttttatag gagatgactt ctatgatgct attataaatt ggaataaaag aagatttgat
     5521 gtatatatag aaaaggattg gataaaatta acttttggta ctgtttcaac ccttcattat
     5581 atagtccaag catatactaa agaaggagag ggtgtattga taaatacacc tgcttatgac
     5641 ccttttgcag aggctgtagt aaataataat agaaagttgt gttgtagctc attaaaatta
     5701 gataacaata gatattattt agatttagag gatatggaaa atcaaatgaa atacgagaat
     5761 ataagattgc ttatattctg tagtccacaa aatccatcag gtcgtatatg gacaaaagaa
     5821 gaactatatc aagtgtcaga gttatgttta aagtataatg taattttggt aagtgatgaa
     5881 attcatagag atataatatt taaagattat agatttgtat ctttgtggaa tgctcatcca
     5941 gaaatttata aaaatagtat aatctgtgta tctcctaata aaggcttcaa cttaggtgga
     6001 ttaaaaacat catatgtatt aataagagat gtaaaaatta gggagacttt attagaaagg
     6061 ctaaaaagta attcaataac ttctccaaat gtatttgcaa taccagcaat agttgcagca
     6121 tataatgaaa gcgaagaatg gctagatgct atgactagtt atgtagagga gaattttgaa
     6181 atagtttatg atttctttga aactaatata ccaaaagcta aagtgatgaa gtcagactct
     6241 tcttttttag cctggataga tgtaagagaa gtatttagaa atgaagaaga atctaaagaa
     6301 ttttttagac atgcaaactt aactatggta gttggaagtt attttgtgaa agatggagat
     6361 ggatttattc gtattaatgt ggggtgtcct agagaaacat taaatgaggc tctaaataga
     6421 atcaaaaaaa cgtatataag tatgtattgt taaatcataa aaaaagtatc tatggtttat
     6481 ataaaaacac attatttagt tatcaaatat tactggaatt ttgtattcca gtaatatttt
     6541 ttgattaagg aatattattg aggaatatta aataatatta tttcataccc ttatattaac
     6601 ttatataaaa atattttatt attttgggaa cgggttagct aacctttatc tagtgtatat
     6661 ttatattgta agaagcagta aaaacttttc ctttactaag tttattaata aacttcttat
     6721 ctttataaat tttgttagaa tttatactat cagcaagacc ataccaaggt tctatacata
     6781 aaaaaggagc agtagaatta gtttcactat aataaggagt ccaaatacca actagaggga
     6841 aatcttccat agatatagtt acatatttac taccatcatt attacatata gacacttcat
     6901 ctatgttggt gtatatcaaa gcatcattct tgaatacttc tggatttaac tgtaagtttt
     6961 ttaattttcc aataggtatt atatcatctg taaaagaacc atttaaattg attttttcta
     7021 catcattttt acttttaaac tcaaggtagt gtttagaaaa atcattttgt tcgtaaaaag
     7081 gaaggttgaa tgcaggatgt ccacctatag aaaagaatat atctttagaa tcagtatttt
     7141 ttacaatcca ttctacctca atactattat catttaatgt atagtttatg attagctcaa
     7201 aagaatatgg atacttcttt aaagtagatt cattatctgt taatttataa gttacaaatg
     7261 tatctccttt atctacaatc tcaaagtcca tatctctagc aaacccatgt tgagtcatgc
     7321 tatataaatt ttcttctatt attgtttcat tatcaaaaag tttacctact atagggaaca
     7381 atataggtga ttgtctaccc caatattttt tattaccatc ccaaagaatg tttttgtcgt
     7441 attttttaga aaatatttta gttagttcag caccaaaact gcttgattcg ataattaaat
     7501 tttcattttg taatatattc atttaaaatc tcctttatta tattttatga gtatattata
     7561 ctataaagta taaagattat taaattttat ttgatattat atttgaaagg tagattttag
     7621 tatattttga aaattttgtt ttgaaaattt gggattattt aagaaatttt gtattataaa
     7681 tagaaggaaa tagataaatt gtatagaata aaatatatta agataagtta ataatgttaa
     7741 atgggaagtg agtttatgga aaaaccaaat acaaaagttg ttaactttca agagtataaa
     7801 gacagaaaaa accaaagaga agtaaataga gacgaacttt taaagcttat aaaaaaaatt
     7861 gtgacttcaa aatagggagt tgttaaactc cctattttaa tgggtttcat aataattaag
     7921 acattctaag tccatctata taatctttga attgtgagaa atctgattca ctacttaatt
     7981 ctatggtttc tgaaccttcc gtataaaaat tcttaagata gatagtataa ttattatcaa
     8041 cacaatcatg ataaatacat ctatatccat cttcatacaa actttttaac ttagaaaaat
     8101 catccacaat atcatctcct taaagttctt ttaaatatat ttttacaaaa agcatctaat
     8161 tgtatgcaaa ttgacaatgt aaaataaagg aaatgaacat aaaatgtaga ataattttta
     8221 tatgtatgta aaaataaaaa gaaaaagttg aaatttttag aaaataagag tattttttat
     8281 ctaaaaagag agtttaaaaa ataaaaaaat gttgggagga taaaaaatga gtttattaga
     8341 aagtatatca aaaaacatat attccctaga taactcttct attgaaaaaa caaagcagag
     8401 attggatagg cttatacatc caactggaag cttaggaaag atagaagata tttgcatgca
     8461 attagcagga atatttggca atgaaaattt tgacacaagt aaaaaggtta taattgcttt
     8521 tgcgggagac catggtgtat atgaagaagg cgtagcacct gaccctcaaa atataacaaa
     8581 attacagttt ccaaattttt cgaaaggtct atgcggagtt ggagtaataa gtaaatttgt
     8641 aggggcagat gttgtagctg tagatgtagg aataaactgt gatgaaaaac tggatggggt
     8701 attagactac aagataagaa aagggacatc aaatatggca aaaggtccag ctatgagtaa
     8761 acaagaagca attagatgtt tagagatagg tattgaaata gcagagcagt gtatagaaaa
     8821 agattataag gttattggta ttggggagat gggtatagca aatactactc caagtacagc
     8881 aataatatct gtaatttcag ggtgtgaccc gttagaagtt actggtatag gtgcaggtct
     8941 taagaaagaa agactaaagc ataaggctga agtaattaga aaggctattg aaattaataa
     9001 tccaaatcca actgatggag tagatatttt atctaaagta ggagggtttg aaataggctc
     9061 aatggcaggt gttatattag gttgtagtgc taataggatt ccagtagtaa ttgatgggtt
     9121 tatttcttat gcagcagcat taattgcata taagataaat ccaaaaacta gagaatatat
     9181 gatagcatct catttatcag cagaatcagg aactaaaaga gcattagaca tattaaaatt
     9241 agaccctcta ttaaatatgg atatgagatt aggtgaggga agtggagctg ctttagcatt
     9301 tgatataata gaagcttcaa actatactta taaaaatatg gcaacttttg atgaaattga
     9361 catgggaaga taaatataaa aatttaatat gtaggagtgt tttgatgagt aaaattattc
     9421 tagtaacagg aggagctagg tcagggaaga gtaactttgc agaaaaacta tgttttgaca
     9481 gaaataataa tacagcttat atagcaacct ctattccatt tgatgatgaa atgaaagata
     9541 gagtaaaaaa acatcaagag aatagaccta gaaactggaa tacttatgaa atatatgaag
     9601 atatatattc cataattaag gagatatata aagaacatga aactgttata ctagattgtg
     9661 taacattatt agtaaataat ctaatgtttg aacacaatct aaatatagaa gaatctagtc
     9721 aagaagaaat aaacaaatta gaacaataca taaaagaaca agttgtcaaa cttattgaag
     9781 aaatagaaaa aacaaatcta tactttgtat ttgtaacgaa tgagttaggt atgggaattg
     9841 ttcctgggaa taaacttagt agaatttatg ctgatattgt aggtcgtata aatcaatata
     9901 tagcttcgaa aagtaatgaa gtttattttg tagttagtgg catagctatg aagataaagg
     9961 agtagtatgc aattatgaaa agatttattt taatactaca gtttttgaca aggataccta
    10021 taaaattaaa tgtaggattt gatgatgaat tttataagtc aatagtatat tttccacttg
    10081 taggttttgt cataggtata ttatcctatt taataggatg gatttctatg cttttatttg
    10141 aaccatttat agcttctata ataataactc tagctggtgt cttaattact ggaggacttc
    10201 atatagatgg attaggagat acatttgatg caatatatag ttatagggat aaagaaaaga
    10261 tgcttgaaat aatgaaagat tcaagattag gtacaaattc acttctagca attatgtttg
    10321 tacttttatt aaaggttgga ttcgtttacg atataataag taataattca ttgtgggtta
    10381 taatttttat gccaatgatt gcaaggttag gagtaatgtt attgacatat aaaactgtga
    10441 caccaagaga aaaaggaatg ggaaatttat ttataggaaa attaactaca agtatgttaa
    10501 taactgctat aatatacaca ttattgatag ttgctcttat tactaaattt atatttttat
    10561 tacctaatat agtgttgatt aaagtattag gttcgattat agtggttttt gtatttataa
    10621 tattattcaa aaaacatatt tataaaaaaa ttgacggagt aacaggagat atattaggct
    10681 gtggaataga actttcagag cttgtatatt taatttacat atatttgtta atttttatgt
    10741 ttttttaaag taaaaagtgg tgataatatg ataagattaa tattaataag acatgcactg
    10801 acaaatgata ataaaaaagg aagattatca ggccatataa atagttgtat tagtgaagag
    10861 ggcaaactac aaataaataa aattaccaga tatctgagta atgaaaacat agataaggtt
    10921 tatacaacac catctacacg tactaaagat acagtagaaa aaatatcaaa attgaaatta
    10981 ttagaaatag aagaaaaaga agctttaaga gaaattagtt ttggagactt tgaaggaaga
    11041 acttttgaag aaattaaaat caaatatcca aatgagtttg aaaaaatgat aaaagaaggt
    11101 aacaactata gatatccaaa tggagaaagt ttgatagact cttataaaag agtggcaaaa
    11161 gaaattgata atataatttt agaaaataat agtaatttag atacaaagac aatacttata
    11221 tgttctcatg caggaacaat aagaaatata ataacacatt tgatttctgg tagttataag
    11281 tatcactgga actttaaaat tgataatgca tctattacag ttttagaaat tgatggtgga
    11341 tttgctgtaa tagataaaat gaattttaca gacttcatat aatttaatca aaaccaaaca
    11401 ttacaatctg tgtttgtaat atatctgtaa aattagcaat gaaaatagaa tattagatta
    11461 aaattaggtt cttaattaga tgttaaaagg gaaaaaggtt agatgccttt gcagcccccg
    11521 ctactgtgaa accaacgaaa ctataaatac cactgtcagt ttgatgggaa ggttatagaa
    11581 gtaggatgaa gttaagccag gatacctgcc taatttaatt taaaacaaga ggtcttcggg
    11641 gtgaaagatt tttaattaat atatataaaa gtcctatctt tagaagtatt aggacttttt
    11701 ttaatttgtt ttttagtaaa ttttaacaag gagggatgga aagtggggaa aaaaataatg
    11761 ctacaaggaa cagcttcaaa tgtaggtaaa agtgtcttag tagcaggttt gtgtagaata
    11821 tttaagcaag atggttatac tgttgctcct tttaagtcac aaaatatggc tttaaattct
    11881 tttataacta aagaaggatt agaaatggga agagcacaag tatttcaagc agaggctgcg
    11941 aaaatagagc caatagctga tatgaatcca gttttattaa aaccttctgg aaataataaa
    12001 agtcaagtta ttgtaagagg taaagttgta ggagaaatgc cttcttctaa gtatcatgat
    12061 tataaattgg agttagtaga tgtgttgaag gaaacattta atagcttaaa ttcaaggtat
    12121 gatattgtag ttatggaagg ggcaggaagt aatgcagaga taaacttaaa agatagagat
    12181 atctctaata tgggtatggc agaaatagct gatgcacctg taataatagt cggagatatt
    12241 gatagaggag gcgtatttgc ctcattagca ggaacaatgc ttttattaaa tgaagatgag
    12301 aaaagaagag taaaaggtgt aataatcaat aaattcagag gaaaaaaaga gcttttacaa
    12361 gatggtgtaa agatgctaga agacatcata aaagtacctg ttataggagt agtaccttat
    12421 acagatataa aaatagaaga tgaagacagt gtaactacga gatttaagaa aaaaatgaat
    12481 aaaagtgata tacacataga aataattaga accccacata tgtcaaattt tacagatttt
    12541 aatatatttg agacacaaga agatgtaagt ataagatatg tagactatgg agaatcttta
    12601 ggaaatccag atatattaat aatacctgga actaaaagta ctatagacga tttaacatat
    12661 ataagaaaaa gtggtcttga atcgcaaatc aaaaatttac atagtcaagg taaattgata
    12721 tttgggatat gtggtggata ccaaatgtta ggtaaaaaat taaaagaccc ttatcatgtt
    12781 gaaagtgaaa ttgaagaagt tgatggaata ggtcttttag atacagaaac aacgtttgag
    12841 aaagaaaaga ctacaacaca agtagaagct actattgttg aatgtatcag tgaatacatg
    12901 aataacctta agggaaaaaa agtaaaaggt tatgaaattc atatgggaat tacaaagcga
    12961 ggtaatgatg taagaagttt agatttaata actaaaaaac ttgataaaga agtgaattat
    13021 actgagggaa gtgtaaataa agaggggaat gtaattggga cttatcttca tggaatattt
    13081 gatgaaataa gctttacaag agcaatttta aacaatataa gaaagaaaaa aggtttagga
    13141 gaattagaaa gcaaagtaaa ctcttttaat gaatttaagg ataaagagta tgataagttg
    13201 gctgattttt taagagaaca tttagatata gagaaaatat atgagattat gaaataatga
    13261 tgacctttat ttataattta ggtaggaggt gtattatgaa aaaaatttta attgcgggta
    13321 ctaatagtgg agtaggtaaa acaacaatat cattaggaat aatgcaagct ttggttaaga
    13381 gaaatatgaa agtacagcca tataaagtag ggccagacta tatagaccct tcatatcaca
    13441 cttttataac tggaagacat tctagaaatt tagattctta tatgttagat gatgaaaaaa
    13501 taaagtatat agtaaataag tcctcaaaag atgcagatat ttcagtaata gaaggtgtta
    13561 tgggcttata tgatggtttt ggaatagatt tagataattg tacaagttca catacttcaa
    13621 aggtattaaa gtcacctgtt atattagtta taaatggtaa atctatggca gcatctagtg
    13681 ctgctatggt acttggatat aaggaactag ataaagatgt aaatataaag ggtgttatag
    13741 taaataatgt aaagactaat agccattatc aaataattaa gagttctata gaaaaatatt
    13801 gtaatgttga agtattgggg tattttcctc caaataacaa attctctttg gaatctcgac
    13861 atctaggatt agttcctagt gtagaaatga aatcactaag ggaaaaattt tataccttag
    13921 cagatgaaat tgaacactat atagatattg acagaataat tgaaatatca gaaagtgatg
    13981 aatttgagag tgattttgat tttcaaactt tgatagaaaa taataaaata aaagaacatg
    14041 ttaataataa atcaatagct atagcatatg ataaagcatt caatttttat tatagagaaa
    14101 atatagagct tttagaggaa cttggtttag aaataaacta ttttagccct cttgaagatg
    14161 catcagtgcc aaatgcagat tatatatata ttggaggagg ttttccagaa atatttggaa
    14221 aagaattaga agcaaatgag tcaatgagaa cttccatatt agaagcacat aacaataata
    14281 tacctattta tgcagaatgt ggaggactga tgtacctagg tgagaaacta ttgaatcaag
    14341 aagaacaaga atttaatatg gttggaatat ttagtggaat tagtaagatg acatcttctt
    14401 taaagagatt tgggtactgt attggtgtag ccaaggaaaa tactctttta tctaaaaaag
    14461 gggaatctat taaagggcat gagtttcatc attcaatatt tgaaagtgat gaaaaatgtg
    14521 tatattcaat gagaaaagaa agagatggaa atatagtaga agagtgggat ggtggttata
    14581 gtaaaggaaa cactttggct acatatcttc atactcattt ttataataac ttaaattgta
    14641 tagaaaattt tttgaaaaaa ggaaatgaat aaatgagtat tttatctatt tatataggat
    14701 atgtgacaga cttaattgta ggagacccat attcttttcc tcatccagtg agatttattg
    14761 gtaaacttat aaattttact caatgtataa taagaaaggt ctctaaaaat gataggcagt
    14821 taaaatttgg gggatttata ttatggctta taaccgttgg aataacatat ttaacaactt
    14881 atgctatagt gaagttattt agttttaata tattattgag tattgtagtg aatagtttta
    14941 taatttatac aacactagcc acaaaatgtt taaaagacga agcattaaaa atttataatg
    15001 tattaaaaac tgaagatata gaaaaatcta ggattcagct atcttatata gtaggtaggg
    15061 atactactaa tttaagtgaa cctgaaataa taagagcaac tgtagaaact gttgcagaaa
    15121 atactgtaga tggtataatt gcgcctatgt tttatgcatt tattggagga gcaccacttg
    15181 ctatggccta taaagctatc aatacattgg actcaacagt tggatataaa aataaaaagt
    15241 ataaagatat tggttttgca tctgctaaga ttgatgatat agccaattat ataccagcta
    15301 gaatatctgt cattcttatg acaattggaa gtttcttttt aaattataac tataggaact
    15361 gttttaaaat ctctattaga gatagaaaga atcataaaag tccaaattgt gctttttcag
    15421 aaggtgcagt atctggtgct ctaggaatac aactaggagg taccaacgtt tattttgggg
    15481 aaaaagtata taagccaaca ataggggata agcttaggga gatagataaa gaagatataa
    15541 ttaaaactaa taaaattatg tatgcatctt catttgtatc tatgttggta tttacactaa
    15601 tatatgtgtt atgtaggatg attttaatta acttaattat ataaattatt gtttgttaac
    15661 ttaaggggga catagatgaa ggatttaggg catggtgcca atgtagatga aatggcaaga
    15721 ttatatggga aaaacccaaa agaaataatt gattttagtt caaacataaa tccaaatgta
    15781 ttaccaaatt tagagagata cattttaaaa ggattagaag agtgtaggaa ttatccagat
    15841 ataaattata caaacttgag agaaaatata tcaaaatata tagatataaa tccagacttt
    15901 ataatacctg gcaatggtgc aacagaagtg atttatttgc ttatgaagag tattaaaaaa
    15961 aaattggcta taataaatcc gactttttca gagtatagaa gaagtgctga gttaaataat
    16021 ttagatatta tagatttaga attagattta gaaaataact ttaaacttaa tatagatata
    16081 ataaaagaaa atataaaaag atttgatagt ttatttatat gtaatcctaa taatccgagt
    16141 ggtaatgtcc aagatttaaa ggaacttgtt catttactag ataaacataa taaggtacta
    16201 ataattgatg aaactttcat ggaatttgtt gaagatgaaa gtaaatatag tttggttaaa
    16261 tatatagaat caaataaaaa tatatttata attaaagctg tgactaagtt ttttggtatg
    16321 cctggtttaa ggctaggata tggtcttaca agcaacactg aaattatgaa caaaatttat
    16381 gaacacaaag agccatggac tatcaattct tttgcagata tactatccaa ttttattttt
    16441 gaagataaag agtatattaa aaatagtaaa gaatattaca tagaagaaag aaaatatatg
    16501 ttacaagagt tgagaaacat aagaaatata aaggtctatg atacagatgc taattttatc
    16561 ttgataagaa tatataaaaa aacaactaaa gaattgaaaa aagatttgtt taagcaagga
    16621 aacatattag ttagagatgc gtctaatttt ataggattag atgatagttt tataagagtg
    16681 gctataaaat ctcatgaaga taacaaaatt cttatagaaa atatgaaaaa tttattgggt
    16741 gattaaaatg aaatcttatg gaatatgccc agcatcttgt ggagaatttg tacaaggtat
    16801 aattgatgat gaagaatatt tatgttcata tgcaatagat atgtattcta aagtgtatat
    16861 tgaagaaaaa ttagtagata taaatttagg gagatataag tcaaggttag caattgaaaa
    16921 ggtatttgaa aaatttaatt tacctaaaaa atatactaaa aatatttctt taaatataaa
    16981 tagtaaaata cctgttggaa aaggaatggc aagttcaact gctgatatag gagctactat
    17041 aaaggcaaca ttatcattga tagataaaga tttaagtagc gaggagattt ctaaattagc
    17101 agcagaaata gaaccaacag attctatatt tatagataaa aatagtatat ttaatcctct
    17161 aaatggaact gttataaaat acctaggaaa tctgaccaat gcgaaagttg taatacttga
    17221 accaaacaaa gtactagata ctatgaagat tagattgagg caagattata ataaattaaa
    17281 agtagagaat aaagaagtaa taaaaaaatc atttgctctt ttagaagaag gattgaaaaa
    17341 aaataattta tctctagttg gagaggcttg tactttgagt agcttagcaa atgagaatat
    17401 agaaaaaaaa gagtgtttaa atgaaataat aaaaatatca aaaaaatatg gagcatatgg
    17461 agtaaatatt gctcacagtg gaactgtagt aggtatttta attgataagt ctatgaatga
    17521 taaaaagatg atagatgcct tatgtgaatc taacataaat tctgtctata ataaaattta
    17581 tacacaaaac ataattaatg gaggaattaa gggggaaata gaatggaata cataaagaat
    17641 cctatgaaaa tagaagaaaa aagttttgaa ttgatacaag aaataataga cgaaattaga
    17701 ccagattata aatttaagaa taatatcgaa gaaaaaataa taaaaagagc tatacatact
    17761 actgctgatt ttgattactt agatatatta aaaatatcag aagaagcagt tgatagtata
    17821 atagatgcat taaaaaataa tgcaagtatt tatactgata caaatatggc tcttagtggt
    17881 ataaataaaa gaaagttaga atcattagga tgtaagtata aatgtttagt ggcagatgat
    17941 gaaactgtaa aattagctaa agaaaaagga attactcgtt ctatggcagc agtagaaatt
    18001 gcagctaaag aagaaggaag aaaaatattc gttttaggta atgctccaac agctttatat
    18061 aaagttatgg agatgaaatc agaaggtaga ttagatttag atgcagttat aggagttcct
    18121 gtaggttttg taggagcaca agaatcaaaa gatgaagttg aaaaaacaga tataccatat
    18181 ataatttcta aaggtagaaa aggtggcagt aatttagcag cagcaatagt aaatgctata
    18241 ttatatacta tgtaggtacc tatatggaag aatatgtata tatagatgga aaaaaatata
    18301 gaagaggata tactacaggt tcatgtgcaa caggtgcatc taaggctgct gtatatatgt
    18361 tgattacaaa aaatagaatc aatactataa atatagatac tcctaagggg ataccattac
    18421 tactaaaagt tgataatata aatatttctg atacatttgt ggaatgttcc ataaaaaaag
    18481 atggtggaga tgatatagat gcaactcata ccatggatat ttatgcaaga gctgaaattg
    18541 tagctaaaaa tgataagaac aaagggtatc ttactctaaa ggatatagat agtttaagca
    18601 ccaatagtga gtgtaaaagt gagctctata agtttattag ggtttatggc ggaactggta
    18661 ttggtgtggt tacgaaaaaa gggcttagtg tggatgttgg taagcctgct ataaatccga
    18721 ctccacttaa aatgataaat catgaaataa gaaagcttat aggagataat tttgaatcta
    18781 tacttggaaa tgataaagtt ttaaaaataa caatctttgc tccacaagga gaaactgtag
    18841 caaaaaaaac atttaatcca agattaggta tagttggagg aatatctata ataggtacaa
    18901 ctgggattgt tgaacctatg agtgatgagg gttggaagaa gtctctatct atagaacttc
    18961 agatgaaaaa agaacaaggc ttagataaaa taatattggt tccaggaaat catggagaac
    19021 agtttataag agaaaaatta aacttagata ttaagtatgt agttagggta agtaatttta
    19081 taggctatat gattaaagaa gctcaaagaa taggatataa gaaaatctta atggcaggtc
    19141 atataggtaa atttataaaa gtatctgctg gtatttttaa tactcatagt aaggtagcag
    19201 atgctagaag tgagatatta gtggcaaact tagctttaat gggagctaga tacgaatttt
    19261 taaataaaat aaatcaatgc gtaactactg aagaagctgt agagctaata aataatagtg
    19321 aatatagaga agtatataat atattaagta ataaatgtag ggagagagta aagcaatact
    19381 tgaatgaaga ttcagatgat attgatgtgg aagtaatcat attttcaatg gataagtctt
    19441 tattaggtaa atcggataat acagatgatt tagtggaggt ctttatatga taaacataat
    19501 aggtctagga cctggcaact tagactatat aacaaaaaaa ggtgaaaatt taatttccac
    19561 ttctgatgta ttaattggtg gtaaaagaaa tttagagtct ataaaaaatt ttgaaggaga
    19621 aaaaatagtt ctggattcta atttaagaga aattatagag tacataaata acaataaaga
    19681 aaagcaaata tcaataatag catctgggga cccattaata tatgggatag gtagatatct
    19741 atctaaaaat attgataata agatgttaaa catggtttct gggattagtt cattacagta
    19801 catattttca aaaatatatg tagatatgaa tgatgtatat attacaagta gtcatggtaa
    19861 agtaccagat tttgattaca tattatcaca taaaaaagta tgtatggtta cagattcaaa
    19921 gataggacca aaacaaataa gtagagaaat aatagataga aatctaaata aaattattgt
    19981 tgtaggagaa aatctgtcat atgacaatga aaaaataaca atagcaaaac cagaagagat
    20041 aataagaata gacaattttg atatgaatgt tgtggtgatt ttagatgagg aatagtgaat
    20101 ttataacagg taaagttcca attactaagg aagaggttag agcaatatca ataagtaagt
    20161 taaatttagt aaatgctaaa aacctcatag acataggggc tggtacagga agtgtaagtg
    20221 ttgaagctgc atataattat cctaatttaa aggttatatc tatagagaaa aatgatgatg
    20281 ctatagagct tatagagaaa aatataaaaa aattcaactt aaaaaatata gaagtgataa
    20341 aaggatgtgc tccaattaat ctagggttaa atgttaaagt tgagtctatt tttcttgggg
    20401 gaacaggaaa taatctggaa gaaataatag aatggtctaa aaaaaatctc gttacaggag
    20461 gaagacttgt agctaatttt ataataatag atacatttaa tcaaacttta aaactgctta
    20521 aaaagcatgg atttaaagaa atagatgtat gtgttttgaa tgtttctaaa ctggagaaac
    20581 taggaaaagg tgaatatttt aaaccactaa atcctatata tataatatct tgtgaaaaag
    20641 gggaaaatga tgatgaataa agtacatttt gtaggagcag gacctggaga taaagagctg
    20701 ataacattaa aaggatacaa attattaagt aatgcagatg tagtgatata tgcaggttca
    20761 cttgttaatc ctgaactttt ggaatactgt aaagaagatt gccaaataca taatagtgca
    20821 catatggatt tacaagagat aatagatgtt atgagagaag gtatagaaaa taataaatct
    20881 gttgtaagac tgcaaacagg tgatttttct atatatggtt caattagaga acaagtggaa
    20941 gatttaaata aattgaatat agattatgac tgtactcctg gagtaagttc ttttttaggg
    21001 gcagcatcat ctttaggggt tgaatataca gttccagaaa tttctcaaag tgtaattata
    21061 acaagaatgg aaggcagaac tccggttcca gagaaagaat caatacaatc atatgctaag
    21121 catcagacat ctatggtaat atttttatct gttcaagaaa ttgagaaagt tgtgtctaaa
    21181 ttactggaag gtggatatcc aaaagatact cctatagcag ttatatacaa agcaacttgg
    21241 gcagatgaaa aaatagttaa aggaacttta agtgatatag ctgtaaaagt taaagaaaac
    21301 aacataaata agactgcttt aataatggtt gggagatttt taggagaaga gtataataat
    21361 tcaaagttat atgataagga tttcaaacat gaatatagag ggtaatattg ctataatctg
    21421 tattacagaa aatggaaaaa aattagctct aaagataaat tctctagtaa ataactgtgt
    21481 tatatatcaa gtaaaaaata aaaaaagtaa tttagttgtt gaagaaacat gtgtacatac
    21541 agttcaaaag aagttaagtg attttgtagg agaaatattt agtaaatttg attacatagt
    21601 atttataatg gctacaggta tagttgtacg aagtatagca ccatacataa taagtaagtt
    21661 tagtgaccct gcaattttgg taatggatga aaaaggcaaa aacgtaataa gtttattgag
    21721 tggtcatatt ggaggggcaa acagcttgac acagtacata agtagtctta ttgatgctaa
    21781 tccagtaata acaacagcta ctgatataaa tcaaaaagct tccttagata tgatagcaaa
    21841 aaaactaaat gcacatattg ataattttag agaaagtgta aaagaagtaa attctatgct
    21901 agtgaataat aaatgtgttg gattatatgt tgatgaagga tatcaagttg atactagggg
    21961 atttaaactt atagaaagta aggatgagtt aagagatagt ttttcaaaca catatataga
    22021 aaaattagtt gtagtaagca ataaaataaa ttgtattgaa gatattgaga atttaaacct
    22081 agataataaa tttgaacatt gctatagtac ccaaaaaata ataaaggtaa ttccaagaga
    22141 tatagtgata ggaattggct gtagaagaaa cacagatagt gaactgatgt ataattcact
    22201 tgaagagttt cttaataaac aaaatataga tattagagca ataaacacag ttggaagtat
    22261 agaactgaaa aaagacgaaa aagcaataat tgaattatca aaaatattga aggtgccatt
    22321 taaaacattt tcagtagatg aaatatctaa ggttgataat ctttttgata aatctgattt
    22381 tgttaaaaaa aatgtgggtg tgtattcagt agcagaacct gtagcatatc ttttgagcaa
    22441 aggtaatttg ttagttgaaa aacataaata taaaggaata actttttcag tggggagaat
    22501 aaaaatatga tatacgttgt aggaattgga ccaggaagta aagatacaat gactttagaa
    22561 gcaataaaag caatagaaga ttctgaggct attgttggat ataagactta tataaaacta
    22621 atagaagaat ttatacaagg taaagaaata atccaaaatg gtatgaggca agaagttgat
    22681 agatgtaaac aagcagtgga ggaagcaaaa aaaggaaaaa aagtagcagt tgtaagcagt
    22741 ggtgatgctg gaatatatgg gatggcaggt cttattttag aattaatatc taaagaagat
    22801 gaagatataa aagtaaaggt aattccaggc gttactgcaa gtattggtgc agcagctata
    22861 cttggagctc ctattatgca tgatttctgt catataagtt tgagtgattt aatgactccg
    22921 tgggaagtaa ttgaaaaaag attgagattg gctgctgaag ccgattttgt aatttgtttg
    22981 tataatccaa gaagtaaggg aagaagtgaa catctaagca aagcattcaa aataatgggt
    23041 gagtttaaag gtggagatac acctgttgga gtggtaaaag atgtaggtag agaaaaagaa
    23101 gaaaagtttg tatgtacttt tgaaaatatg gactttgaaa aagtcgatat gactacaatg
    23161 gttataatag gaaataagtc tacctatatt aatggtgaat tgatgataac tccaagaggt
    23221 tatacagtat gattttaatt ttaggaggaa cttcagatag tttatctata tgtgataaga
    23281 taaatgaact gaaaaatcaa ccatatatcc tttcagttac tacagattat ggaagggaac
    23341 ttgcacaaca acatgcagag aatgtaattt taggtaagct tggaaaagaa gatatgctaa
    23401 aatttataaa agataataat atagtaaaaa taatagatgc cactcatcct tatgcagttg
    23461 aagtatccaa aactgctatt acttgtgcaa aactattgaa tatagattat ataaggtttg
    23521 agagaaaatc tttaattgaa gaaatagatt atgataataa atttatagta gatacaattg
    23581 aagaagcctg tgaaatagca aataaaatag ggacaaatat attcatagga acaggaagta
    23641 aaaatttgaa cagatttata gaggaaataa gtgataaaaa tttagttgtt agagttcttc
    23701 caacgagtga agtcatatta agttgtgaaa aattaggttt aagtgcagat aatatagtag
    23761 ctatgaaagg tccatttagc cagtatataa atgaagaaac atataaacat tacaatatag
    23821 atttagtcat aacaaaagaa agtggaatag ctggtgggtt cttagagaag gtaaatgcat
    23881 gtaaaacttt caatatacca gtaataatta taaaaagaga atcgattaac tacgaaaatg
    23941 tatttaatga cataaataaa attaaagaca tacttgtgta atttttagga ggagaatata
    24001 tgaagaaagc cttattagta gtgagttttg gtacaagcta tcatgaaacg agagaaaaga
    24061 caatagatat atgtgaagat aaaataaaaa attcgttaaa aagccatgat ttttttagag
    24121 cttatacatc aaatatgata ataaataaaa taaaaaaaag ggatggaata gaaatagata
    24181 atccaataca ggctttagat aaaatatatg aacaaggata tgatgaagta ataatccaaa
    24241 cacttcatat tatatgtggc gaagaattta acaaattaaa agaacaagta gatggatatt
    24301 cttctaaatt caaaaagatt gtattaggaa gacctttgct tacacatatt gaagattatc
    24361 aggaagctgt agaggcaatt aagcatcaaa ttcctcatat ggaaagtaat gaagcagtag
    24421 tatttatggg acatggaaca cttcatgaat cacattcagc gtatccagca ttggaataca
    24481 tgcttaggga taatggaata aatgcttatg ttggtacagt tgaagggtat ccagaaatag
    24541 aacatgtaat aagaagatta aaagaaggaa atataaaaac tgtaaatttg atgccattta
    24601 tgttagttgc aggagaccat gctataaatg atatggcagg agatgaagaa gattcttgga
    24661 aaacaatatt agaagaaaat ggatttaacg taagagttca tttaaaagga ctaggagaaa
    24721 attcttatat acaagataaa tttgttcgtc atgctgtaaa atgtgcagag aatgcgaagt
    24781 tctatggtat aggagcaggg ccaggagatg gttctttact tacgataaaa gcggttaata
    24841 ctttaaagaa actggatatt ctatacactc cagaatcaaa aaagggtgga gatagtctag
    24901 ctttatcaat agttagtgaa tatttacctg aatcagtgga aataaagtct aggcatttcc
    24961 caatgagttt tgatgggaat gaaaaaagtt tagcttggga caatgtggca caagaaatta
    25021 tatcagatgt taatgatggt aaaaatgtag gatttgttac attaggagac cctatgattt
    25081 atagtacata tgtatatata atgaaaagaa tattaggaga ggtagaagtc gaaactatac
    25141 caggaatttc atcattttcc aatatagcat ctaatcagaa ttttccatta gtcatggata
    25201 aagaagcttt agtggtaatt ccttgtacta tggaagatga taagatagag tatgctttgc
    25261 aaaattataa ttcaatagta ttgatgaaag tatataaaaa ttttaaagaa ataataaaaa
    25321 aattagaaaa gtatgattta atagaacatg ctattttagt aagtaattct tcccaagaaa
    25381 gcgaaaatgt gtttacagat ttaaaagaag cgcaccttga agataagata tcttattttt
    25441 ctactatact agtaaataaa gataataaaa taaaataggt gaaagcaata tttaaaggag
    25501 acttaatatg aatatagtgg ttggaactag gggtagtaat ctagcgctta tacaaacaga
    25561 atgggtaata aatgaattaa aaaagaagta tccagaaatt agttttgaga ttaaaatcat
    25621 aaagacaaaa ggagatttaa ttcaaaatgt ttcactggat aaaataggtg ataaaggatt
    25681 gtttgtcaaa gaaatagagc aacaattatt agatggaaaa atagacatag cagtacatag
    25741 tatgaaagat atgccttctt atttagcaga tggactaaaa tttgctcata cgcctaagag
    25801 agaagaccca agagatgttc taatacttag agaaggatat aaaaatttag atgatttgcc
    25861 tcatggagca gtaataggaa caggaagtaa aagaaggaag tttcaattat tgaaacaaag
    25921 acctgattta aatatagtac aagttagagg taatgtggaa actaggatac gaaaaataaa
    25981 agatgaaaac atgcatggta ttgtgttggc agcatcagga ataattagag ctaatttaca
    26041 agataaaata agttcttatt tacctgttga tgttgttatt ccagcacctg cccaaggagc
    26101 tttagctata gaaattagaa gcaatgatag tacgatagag ggaatagtaa attcactgaa
    26161 ggatgagaat acagaaatcc aaatattggc tgaaagaggt tttttagatg gtgtaaatgg
    26221 aagttgtcat attccaatag cagcttattg tgaaataatc caagataaaa tacatcttac
    26281 tggcttatat ggagattcag aaggaaaaaa agtagtaatc aagtctatag atggagatat
    26341 ttcttctcct agagagttag gtttaaagtt agctaaactt gtattgaagg agtatgagaa
    26401 ttatgaaggg taaggtttac ttagtagggg caggaccagg tgattataaa ctcatgactt
    26461 taaaaggttt agagtgtata agaaaatcgg atgtaatagt atatgataga ttagcaaata
    26521 gtagttattt aagagaagct aagcctgatt gtgaacttat atatgtaggc aaggcatcaa
    26581 gtaatcatat tttgccacaa gaagatataa atagaattat agcagaaaaa gctaaagaag
    26641 gaaaaattgt aactcgtctt aaaggaggag acccttatgt atttggcaga gggggagaag
    26701 aagcagagac tttagtagat gaaggtatag aatttgaagt agttccaggt ataacttctg
    26761 ctataggagg tttatgttat gcgggtattc ctataacaca tagagaccat gcttcgtcat
    26821 ttcatgtaat aacaggtcat ttgaagggtg atgatagtgg agaattaaac tggaatgctc
    26881 ttgcaaataa caaaggtact cttgtgtttt tgatgggaat ttcaaaccta gagaaaataa
    26941 gtgaaaactt aatgaaagaa ggaaaagata aggatacacc tgttgctctt ataagttggg
    27001 caactagata taatcaaaga gttgttacat ctacacttga aaatgtttat gaaactgcta
    27061 ttaaagaaga agtaaaaccg ccaactctta tagtagttgg gagtgttgta ggacttagag
    27121 aaaagttgaa cttctttgaa agtaagcctt tatttggaaa aagtattgct gtaactaggt
    27181 ctagaaatca aaatagtgta ttggtagaaa aaataatgga tttaggtgga aatcctatag
    27241 aaatacctac tattaaggta gaaaaaatac aaaataatat taacttagaa aatgaaataa
    27301 aaaatataaa taagtataat tatctaatac taactagtaa aaatgccgta gaaatcttct
    27361 ttgaaaagat atatgaaatg aattttgatt taaggatatt atctaattta aaggtatgtg
    27421 ctataggttc tgctacatca aatgagttga aaaaaagagg aattatagcg gatatagtac
    27481 caaaaaaatt tgtagcagag tcactgtatg aagagttagc gcctatttta aatgatgacg
    27541 ataatatact tatacctaga gcagaaaatg ctagagatta tctagttgac aaacttaagg
    27601 ctatttgtga agtaacagaa gtacatatat atagaacagt aatagatgag gagaaaaaag
    27661 gtgagatatt agatatttta aattctaatg acatagatta tataacattt actagttcct
    27721 caacagtaaa taatcttgtt gaaattatag gaaaagaaaa tattaaaata ttagaaaaaa
    27781 taaaaacact atctataggt cctataacta cagagacaat aaatagtttg ggaataaaat
    27841 tgtacaaaga atctgctaca tctactatag attcgttagt agatttaatt ataaaaaatt
    27901 aattttggag gcttgaatat gttaagaaga ccaagaagac taagagcaag taaagcaatc
    27961 agaaatttgg taagagaaac taaggtgaat gtagaagact taatatatcc actgtttgta
    28021 gtagaggggg aaaatataaa agaagaaatt aaatctttac cagatgtcta tcatttatca
    28081 atagatatgc tagaagacga aataaaagaa ataagtgagt tgggaattga acatataatg
    28141 ttatttggtg ttccagatga aaaagatgag tgtgcttcac aagcttttaa tgataatgga
    28201 atagttcaaa aggcaattag aaaggtaaaa gaaataaatt cagatataaa tgtaataaca
    28261 gatgtctgta tgtgccaata tacaagtcat ggacattgtg ggattttaac tgatactggt
    28321 tatgttgata atgataaatc attggagtat ttggctaaaa tatctgtaag ccatgctaaa
    28381 gcaggagctg atatggttgc tccatctgat atgatggatg gaagaataca agcaataaga
    28441 gaagctttag atgaaaatgg attagaaaat gttggaatca tggcttatag tgttaagtat
    28501 gcttcaaatt attatggacc ttttagagag gcagctcatt cagcaccatc ttttggagat
    28561 agaaaaacat atcaaatgga cccagcaaat agcaatgaag ctataataga gactgaactt
    28621 gatatattgg aaggtgcaga tatacttatg gttaaacctg cattatcata tttagatatt
    28681 ataagaagag ttaaagataa ttttgactta cctctagcag cttataatgt aagtggagaa
    28741 tactctatgc ttaagtttgc tgtgaaggaa ggtttattaa atgaagatgt aattcttgaa
    28801 tcaattacat ctataaaaag agcaggagca gatataataa taacttattt tgctaaagac
    28861 attgctaaac tgttgaaaaa ataaattcta gtagtataaa gaatatcaaa aagtatagga
    28921 attgtaatta actattatac agtagttttg tttagacatt tattgaagaa taatagaaac
    28981 taatttgcaa tttctatact ttatttatgt aactaaataa aatttggagg tagaagatgc
    29041 tatatcctat taatttaaaa ttagatgagt tagatgtagt tataattggt ggtggagaag
    29101 ttgcatatag gaaatgtaaa aatttcttag agtttaacaa aaatgttact atagtttcta
    29161 aacaaatttt aaataaattt tatgatttaa aaggaaatat aaaaataata aaagatgatt
    29221 ataaagaaat atatataaaa gatgcttctg taataattgc tgccacaaat aatagagagt
    29281 taaatatgga aataggatta tattgtagag caaaaaataa gctagtgaat gtagttgata
    29341 atatagaaat atcgaacttt actgttccat catatataaa aagaggagat ttacttataa
    29401 gtgtgtctac tgggggcaag agtccttcat tatcatcaaa gataaaaaaa gaaattgaag
    29461 agagatatac tgaagattat gaggagtatt taaatgtatt aggagatata agaaaaaagg
    29521 ttataaaaaa atacgaagat aaaagtaaaa gaaaagatgt gcttaatatg ttggtaacac
    29581 ttgacctaga agaacttaag aagtttgata tataaaaaat aaaataaaaa atgttaaaaa
    29641 ggtgttgacg gat
//
LOCUS       DAELLV010000025        27452 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.025, whole genome shotgun sequence.
ACCESSION   DAELLV010000025 DAELLV010000000
VERSION     DAELLV010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27452
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.025"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            206..1081
                     /locus_tag="KPD58_000940"
     CDS             206..1081
                     /locus_tag="KPD58_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HBG0264724.1"
                     /translation="MNVYEKGNGVILEGVTDFDPVHIFECGQCFRWHKQEDGSYTGVA
                     KGRILNVKKENDKIYLNNTNLKEFNSIWYNYFDLGTDYTEIKNKLKNMDEYLNKATEF
                     GWGIRILRQDGWEMLISFIISSNNRIPMIQRAIENLSRKFGKYIGEYEGNEYYAFPTP
                     EELNKASQEEIRACQTGFRDKYIKSTTQAVIENNDEVSEYTNLSTEDCRKELLKFNGV
                     GPKVCDCIALFGMQKYDSFPVDVWVKRVMQEFYIDEDMSLPKMRTYGIDKFKEMSGFA
                     QQYLFYYARELGIGK"
     gene            1099..2589
                     /gene="cls"
                     /locus_tag="KPD58_000941"
     CDS             1099..2589
                     /gene="cls"
                     /locus_tag="KPD58_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBG0264725.1"
                     /translation="MGVIGTIFLFYLIISYLAGAIISVIILLENRDPAKTMSWLLMFI
                     IFPGVGLMIYAISGRNIRKRKLFKTQKLANNIKEKKLFDTLEKITEIVELEKESIKQN
                     KLLRDEEDGSYRKRVINMLLKTGMFPFTKNNKVDVFVDGNEKFKRLIEDIREAKDHIH
                     LEYFIIKDSEIGRVLKEELIKKAKEGIKIRILYDDVGCWRFWFNRKFFREMREVGIEI
                     AAFLPTKFPIIGGKLNYRNHRKIVVIDGIIGYTGGINIGDEYLGKNDKFGYWRDTHIR
                     IKGISVYMLQMTFLIDWYYTTKEVLVTKNYFPSVGNVGESMIQVVASGPDSDWEDIHY
                     AYFSAICQARKNVYIETPYFIPDESLLKAIKSAALSGVDVRIIFPKIADHKIVNIASY
                     SYFEEILRAGGKVYLYNKGFIHSKVVIIDDKIASAGTANMDLRSFMLNFEVNAFIYDE
                     EVIRVMTDDFFEDLSYCEELNLEVFKNRNIIQKIKESVARLFSPIL"
     gene            2782..3066
                     /locus_tag="KPD58_000942"
     CDS             2782..3066
                     /locus_tag="KPD58_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBG0264726.1"
                     /translation="MKIRPLADRVVIKKVEAEEKTASGIVLPGAAKEQPQIAEVVEVG
                     PGGIVEGKEIKMELTVGDKVIFQKYSGTEVKIEGQEYTILRQSDVLAVIE"
     gene            3099..4727
                     /gene="groL"
                     /locus_tag="KPD58_000943"
     CDS             3099..4727
                     /gene="groL"
                     /locus_tag="KPD58_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729227.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBG0264727.1"
                     /translation="MAKEIKFSEETRRALEAGVNKLADTVKVTLGPKGRNVILDKKFG
                     SPLITNDGVTIAKEIELEDRFENMGAQLVKEVATKTNDVAGDGTTTATVLAQAIIREG
                     LKNVTAGANPILLRKGIQKAVTVAVEELKNQSRIVETQEAISQVASISAGDEEVGKLI
                     AEAMEIVGKDGVITVEESQTMNTELDAVEGMQFDRGFVSAYMVTDVDKMEAVLNDPYI
                     LITDKKISNIQELLPVLEQIVQQGKKLLIIAEDVEGEALSTLVVNKLRGTFDVVAVKA
                     PGFGDRRKEMLQDIAILTGAQVISEELGYDLKEADLSMLGRASSVKVTKESTTIVDGS
                     GDKKAIEDRVTQIKHQVEQTTSDFDREKLMERLAKLAGGVAVVKVGAATEVELKERKL
                     RIEDALNATRAAVEEGIVAGGGTAFVSVIPAIGTLIESLEGEVKLGAQIVKKALEEPL
                     RQIAINAGLEGAVIVQNVVNSEAETGFDALNEKYVNMIEAGIVDPTKVSRSALQNAAS
                     IASTFLTTEAAVADLPEKEDAGMPGMGGGMPGMM"
     gene            4989..5729
                     /gene="larB"
                     /locus_tag="KPD58_000944"
     CDS             4989..5729
                     /gene="larB"
                     /locus_tag="KPD58_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HBG0264728.1"
                     /translation="MDLRKLLEEVKNENIDIDFALEKLKDLPYEDLGYANIDHHRELR
                     NGYPEVIYCEGKSDEHILGIIDKMNQKSSNILGTRCRKETFLKIKEIYNHAEYEEASK
                     ILKIQNHDIENIGKGKIVIATGGTSDIPVADEAYHTAKFLGNDVDRIYDVGVAGIHRL
                     LNKRHKIDSARVIVAVAGMEGALASVIGGLVDVPVIAVPTSVGYGANFGGLAALLAML
                     NSCASGISVVNIDNGFGAGYLASMINKL"
     gene            5813..6547
                     /gene="larC"
                     /locus_tag="KPD58_000945"
     CDS             5813..6547
                     /gene="larC"
                     /locus_tag="KPD58_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HBG0264729.1"
                     /translation="MSEKILYFDIISGISGDMTLASLLNLGVPKEIFLEEIHKLRMSD
                     EFNINISSKTENGIVGTKVEVITKEKHTHRNLVDIFEVIDKSTLNENVKSKAKKIFMV
                     IGEAEAKVHGTTIDKIHFHEVGAIDSIVDIVGASILVDLLCVDKVCASTVSVGSGFVK
                     CEHGIIPIPAPATIEILKGVPIKLNNVNGECTTPTGAAIIKVLCDEFVDELEFSPKQI
                     GYGIGHKKFEVPNILRTVLGEKKKKN"
     gene            6577..6996
                     /locus_tag="KPD58_000946"
     CDS             6577..6996
                     /locus_tag="KPD58_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF111 family protein"
                     /protein_id="HBG0264730.1"
                     /translation="MSSEIYSYLYEKILDEKALDIYTESIYMKKNRPATKLSILCNES
                     DLEKFIKILLLETSTFGVRYNAYNREILERKFVKLDTKYGVVTVKLGYYKGKLVKATP
                     EYNECKTIAKNEDIPIKKVFADINFRIKEEFSENLLT"
     regulatory      7056..7157
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            7378..8913
                     /gene="guaA"
                     /locus_tag="KPD58_000947"
     CDS             7378..8913
                     /gene="guaA"
                     /locus_tag="KPD58_000947"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBG0264731.1"
                     /translation="MKHELVLVIDFGGQYNQLIARRVRENNVYCEILPCTASIERIKE
                     KNPKGIIFTGGPNSAYLEDSPTISKEIFELGVPILGICYGIQIMSHVLGGVVRKGNKQ
                     EKEYGKTAITYGKSSLFEGITTNSVWMSHTDLIEKVPEGFTIVANTNDCPVAAMENVE
                     RNLYGVQFHPEVEHCLEGDKILTNFLYNICKVKGDWTTDSFIEDKIKELKEKIGDKKA
                     LCALSGGVDSSVAAVLIHKAIGDNLTCIFVDHGLLRKNEGNDVERIFREKFDINLIRV
                     NAEDRFLSKLKGVSEPEAKRKIIGEEFIRVFEEESNKLGKMDFLVQGTIYPDVIESGH
                     GNAATIKSHHNVGGIPEDVDFQEIVEPLRELFKDEVRKIGLELGIEEGLIFRHPFPGP
                     GLGIRVIGDVTKEKCDILREADAVYMDELRKAGLYREIWQAFATLPDVKTVGVMGDER
                     TYAYLVGLRAVTSSDGMTSDWYKMPYDVLERISNRIINEVDGVNRVVYDITSKPPGTI
                     EWE"
     gene            9452..10882
                     /locus_tag="KPD58_000948"
     CDS             9452..10882
                     /locus_tag="KPD58_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG0264732.1"
                     /translation="MILNQIFIEAGGIMRNKIIIGITFSLVLLMSGCASKQTAEINVL
                     ATTDLHGVIPYELTSYVKEERKKDKNLTLVDAGDFFDSGEVFGSSMDSYFSARYKDID
                     NNTERYIETPISKDMKEVGYDVAVLGNHEFISNNKFYLDNMISDFEKQKINILSANTY
                     KKNGDSYTKPYIIKNINTPEGNVKLGILGLTIKEVGERKQWKDGKLVDSKSLELKDQE
                     GYNGELYMNDLVEDAKKWVSIMEKEKPDIIVAVAHTGEKPKKPRNPGNRIQDLAQQVD
                     GIDAIVAGHNHVQIEQHDYKNKSGENVIVTEPGKHGECISKINFKLEKNNDKWNIVDK
                     SSKLIKFEKNQESDKFGEFISSINKVSKDKKEIRLSEVKTPEWDKIYVFKSGTSADDI
                     YKTIGYKWRNISSPKGDDMVQIVVMNNNKPVSYVYGEAEVMPINIKCDESEYKDNVVT
                     IYPNKNDKFKIEKGKQEYSINLKHIQ"
     gene            complement(11030..11380)
                     /locus_tag="KPD58_000949"
                     /pseudo
     CDS             complement(11030..11380)
                     /locus_tag="KPD58_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            11375..11896
                     /locus_tag="KPD58_000950"
     CDS             11375..11896
                     /locus_tag="KPD58_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBG0264733.1"
                     /translation="MFSIGGGYAIIPLIQEKVVNQTGWISEKMFTDIITISQMTPGPL
                     AVNTSTFVGLQIGGFWGAFAATLGCVLCGVVIALFLYNFFQKHQNSTYIFEILNGLKS
                     ASLGLIISAAATIIILALFGTNKVYFNSIFEMLNWTALIIFFVTLLIVRKWKINPIVI
                     IIISGILGIFFYL"
     gene            complement(12025..12819)
                     /locus_tag="KPD58_000951"
     CDS             complement(12025..12819)
                     /locus_tag="KPD58_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0264734.1"
                     /translation="MIFCNSFITRKSSMNIVVTHTPIVTTLEMQKAYEPLAKHNVNIF
                     AFDFSGTGKSDGYEKDFSRNSIVKDLDSVITYIEKNYSSNIHLYGNTGIGGMFAQYYA
                     CSDNKLKSFAQFACVDYKNTTGVGYTYLTVKLLNFFFKFLPNFHITVKPPKYKGYNEV
                     YDNEFYKIFEKKNPHIWKNSTKIINTMLECFIAEDSKIKDGVNIPTLVFKTLHDRYFK
                     TQYFDRYYNSLKCKKKLVEINNVHNSYYIDSEKFCQEAYDWFVENQ"
     gene            complement(13043..16471)
                     /gene="uvrA"
                     /locus_tag="KPD58_000952"
     CDS             complement(13043..16471)
                     /gene="uvrA"
                     /locus_tag="KPD58_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG0264735.1"
                     /translation="MKINNYKNQSIITNPKKFENKYQDLPKTPIELLKVVQSLVIHGD
                     QGKLYGISFNKQQSDEELLRTIPQMLKRIFEINSNPLTIPRNPKQRLVGMCRDYSLLL
                     VSLLRYRGFEARMRAGFANYFESELTYEDHWLVEYYDTLTKRWIRIDAQIDDIQKNYF
                     QINFDTHDVGKTDGFLTGSEAWIRCRQGHAHPDDFGYNKNWKGWHSVKGNLLHDFNNM
                     IGLELLPWDLWTELSSKKYNQLTRAEKNLLDEMAEILSSGNIKIEDLNLLIEKLPEDY
                     LKSIFSQLKILGISEIKELGNPLELEKKFKFTKSINKSIKNSLCHNKSSIYLKGGRQN
                     NLKDVEVTIPKNQITVITGVSGSGKSSLAFDTIYEEGKRRYFENLSNGAKLSEQLQKP
                     EFDLLQGLTPTIAIEQKKGSQNPRSTVGTLTSIWDYLRMLFVSIGKSYCPYCKIPLEK
                     KNNTKNYCPHCQTIFSKINTSTFNANSHTGACHDCNGLGFTYQVNPQLIVKDPTISIL
                     DGATYYFGKLRGKKPNGNWMVGELYAIAKDKNIDLDIPWNELPRDFIDAILYGTDDKI
                     YEFSFESKGRESKIRRPASGAINHIQRLFRESSSENNTLHQYMNKIPCNTCGGELLCI
                     EARFTTIKGYRFPELTKMTIEQLWNWLCELPNQLQKNELSLVNDILTELKIRVSYLLK
                     VGLSYISTDRTSPTLSGGELQRVRLSSQLGSELVGLTYILDEPSIGLHPRDHNLIIKM
                     IEELRDKGNTVIVVEHDKDTILSADYIIDVGPSAGTKGGFIIAEGTTQEIIKNPNSIT
                     GKYLSTYNKTGSQNKTIPSKWLSLKGCHANNLKNIDVEIPLNCMCSITGVSGSGKSSL
                     VFHSLLPALEEKLKQKSIPDKNYTEFTGFDAIDDFILMDQTPIGKSSRSTPATYINIF
                     DEIRSLFAETPQAKQKLLDESYFSFNSKKGQCPNCQGLGKTKIILQYMADQWVTCSEC
                     QGKRYQKEILSIQYKGKTIADILDMEVAEAKTFFSDCSDIYRKLSLLDEVGLGYLKLG
                     QNTLGLSGGESQRIKLAKELGTKTKKRMLYILDEPTTGLHFKDIENLLITFRKLVNEQ
                     HSLLIIEHNTEVIRASDWIIDIGPDSGINGGEIVASGTPDEIKINPNSITGHFI"
     gene            16790..19024
                     /locus_tag="KPD58_000953"
     CDS             16790..19024
                     /locus_tag="KPD58_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG0264736.1"
                     /translation="MKLSRYKKNSLVLSIILCSLIISILVITVFNTVKLHNISNKQSK
                     NYLNDVSTQIVMNVDSKIKFILSDLRIMADFIKQYEGDSRYEYLSKRKTSYKYYDVGI
                     IDLEGKAEFLSGKKFDLKNTASYKKGIEGKEYSEVIESMGFVLYSVPIFNDNHEVSSI
                     LIGVSNKESMNEILNIDNFNGEGTIEIINSKGKPLFIGKNTELIKDLSHKYKDSTDEP
                     WAQKMLNDFKNDKSGNITITSSKGVQCLLTYHPITRGLNDWHFLLIVPEDAVLGELNK
                     LNSFTITMTFIITCIIGIITLILYIIRRKYVNQIENIAYLDSITNGINSTKFSMLVKP
                     LISESPDSTYMMIAMNIKDFKLINDCFSSEKGNMTLKHLYNILYKNIDENEEFVCRHD
                     ADLFYLLIKNRPVLEALELLYKIEADTNYFNKNRENPYFLRLSVGIYTIENHNEDLIT
                     IQDHANTARKSFNKTHQSDFSFYSDIERKRLISEKEISNLMEKALENKEFFMCLQPKI
                     DIKSGKISGAESLVRWKNSEKGIIYPSDFIPLFEKSGFICKLDLYILEETCKLISKWI
                     KEGKEVLRISVNVSRQHLNDKLFLEKYKHICNKYNVPTCLIDLELTESIFLENPEAID
                     IIEDIHSNGFKCSIDDFGFGYSSLGILKDFKVDIIKLDRSFFVSKNNIDRGKVVIKSI
                     IELSKRLGMKVTAEGIEELEQVEFLKNVGCDYIQGYVFSKPLLISDFENFAYENNKIK
                     NIHY"
     gene            19280..21310
                     /locus_tag="KPD58_000954"
     CDS             19280..21310
                     /locus_tag="KPD58_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0264737.1"
                     /translation="MISGRMLLIMNFLKGKNKTSYKDISKQLSIEERKVRYDINNLNI
                     VLRSLNKSVIQKMNKGVLIIPDDFQVVCINNNEYVFSLTERVSIMKIISMFKIDNLNL
                     EKLSKEFQVSRSSIKNDLNVLSSELERNQITITYDKSFKISGEEDIIYKQRLNILKSY
                     SYLFGKEKSDLSVFEKYLVNVIEKIFKGIYLIDIYNWSMNLLLQMGWTLNDESFTWYV
                     SNIFLFTWYIHSDEKHPLESASLSEPCFENKFDQEFETIIQKKLTKEQIFLLKSFVFF
                     TNKYASLNEDMDLISTEIIVQDLIYNMSEILEISFKEDMILYKGLLNHIAPLIERIKQ
                     NIQIYEELLHVIPDKYQYVLEATKVSIQKNRVLSQIKNENETILLAIHFLGSIHRNER
                     NNYKNILLVCGFGYGVIAMLKDSLVNDYQINIVESIPSYKLQDYKEWESIDFIITTSK
                     IVIDLPKTIIEINPFLQEEDYLKFEEKGIKRKNVLTNYMSIKKRLDFLEEDAQKRVLS
                     IICQELGYSDERILFRQLNLSDLIGIDTIRIIDGDMKWEDAVKFSSNILANCDFVSAQ
                     YAENIIDILKNVGFYAVKDNEFALLHGNDSSLVKVSSISLLICKEAVSFGDKKVKIIF
                     CLASRDKKEQIPAIINLTKMVYKTNFIAMLESAKTKEEAEVLIHKYEKEVTV"
     gene            21307..21759
                     /locus_tag="KPD58_000955"
     CDS             21307..21759
                     /locus_tag="KPD58_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0264738.1"
                     /translation="MNKLVNVKCSFKIHVNSKEEAIISLVDVVSKEGYLIDKNQFLKD
                     VLKREETLSTYIGHGIGLPHSQSVGVKNSCITIGKLDTPIEWTEEGEKVDLIFLISVT
                     KDNENNLHLKILSKLARLLMHESFRNQIRCSDEQTVYNLIKEKIEEED"
     gene            21761..22816
                     /locus_tag="KPD58_000956"
     CDS             21761..22816
                     /locus_tag="KPD58_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HBG0264739.1"
                     /translation="MRILKFLQKHLMTATSYMIPFVVAGGILFALSVTLSGQAAVPET
                     GWLAKLNQIGAAGLALFIPILGGYIAFSMADKPGLAPGMIGAYLAKEVNAGFIGGIIA
                     GFIAGFVVLQLKKIKLAPTMRTLGSIFIYPLIGTLITGGIIVFLIGEPIASFMTWMTN
                     WLNGMSGVSKIPLGGILGGMIASDMGGPINKVAATFAQTQVDTLPYLMGGVGVAICIP
                     PIGLGLATLLFPKKFSKEERDSGKASLLMGCVGITEGAIPFATSDPVRVIPCIMAGSI
                     IGNIMAFLLGCLNHAPWGGLIVLPVVDNRLGYIASVLTGAVVVAVLMKLVKKDVKEDE
                     EIEEDLDESIELIFEEL"
     gene            22883..23191
                     /locus_tag="KPD58_000957"
     CDS             22883..23191
                     /locus_tag="KPD58_000957"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBG0264740.1"
                     /translation="MKIVAVTSCPSGVAHTYMSAESLELSAKKFGIEIKVETQGSSGI
                     DNELSLKDIDEATCVILTNDVEIRNMERFKGKKVVRMSVSEIIKKSDALIKKIKDTFQ
                     "
     gene            23295..24560
                     /locus_tag="KPD58_000958"
     CDS             23295..24560
                     /locus_tag="KPD58_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HBG0264741.1"
                     /translation="MKKLPIKTVVQRLLNLQEEGKSATLLGIGPMSPNLLQASFELAK
                     DDDFPLMFIASRNQVDADELGGGYVNGWNQETFTKDIKKVADKVGFDGLYYLCRDHGG
                     PWQRDKERNDHLPVDEAMELGKKSYLADIEAGFDLLMIDPTKDPFEIGKVIPLDVVLE
                     RTVELIEYCENERKRLNLPDIGYEVGTEETNGGLTSTETYETFITRLKVELDNRGLPM
                     PTFIVGQTGTLTRKTEQVGIFNFRNAYDLAQMAKKYGVGLKEHNGDYLDDVTLLEHIP
                     SQIIATNVAPQYGTEETRAYLKLAEVECKLEKEGLVEKTSNIRHVLLVNAIECGRWRK
                     WVVGEQKNLTTEEIFKDEVLSNEILDIAGHYTFNNDDVKKEIEVLYDNLSKNNIDGQR
                     FVVDHIKRPLRDYAECYNLKGVTTRILNK"
     gene            24648..25364
                     /locus_tag="KPD58_000959"
     CDS             24648..25364
                     /locus_tag="KPD58_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBG0264742.1"
                     /translation="MINLIFDVDDTLYNQLTPFYTAYNKVFSSIKDISIEDLYMSSRK
                     YSDEVFHMTENGEMPIKEMHIYRIMKAFEELGNSITEKDAQSFQDEYIYQQSQITLIP
                     EVERILNFSKERNINLGIITNGPSNHQRMKLKQLNIENWVDKSNIFISSEVGFSKPDT
                     NIFRVAENVMNLDRENTYYVGDSYRNDVLGAKKAGWKSIWLNHRGYEVEELFYKPDFV
                     ILEHKDLISLFIKICSYKNM"
     gene            complement(25559..25852)
                     /locus_tag="KPD58_000960"
     CDS             complement(25559..25852)
                     /locus_tag="KPD58_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HBG0264743.1"
                     /translation="MRNMIENLIKNNTYIDDKVIMLSMLSSAKASANMYLNSALTSST
                     PELRAIYSASLTQMVEGHTALTELSINKGWVKPYDKPIQQLTCSYKESQNVID"
     gene            complement(25858..26073)
                     /locus_tag="KPD58_000961"
     CDS             complement(25858..26073)
                     /locus_tag="KPD58_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264744.1"
                     /translation="MRILSETEKISLAAIIKMESDGLLMQRAINMLISDEDLKRQSES
                     SILATEGRIKAIQQFIVENEILISEEV"
     gene            26521..26919
                     /gene="tnpA"
                     /locus_tag="KPD58_000962"
     CDS             26521..26919
                     /gene="tnpA"
                     /locus_tag="KPD58_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBG0264745.1"
                     /translation="MYKSNKNIVYDCKYHIVFCPKYRKPVLVGEVEKMLKEILPYKAD
                     ELGAEIIEMETDKEHVHLLISCDPQYGIHKVVKGLKGFSSRVLRENFPHLKSSMPSMW
                     TNSYFVGTVGSVSLEVVKQYIENQQVRTKK"
     gene            26916..>27452
                     /locus_tag="KPD58_000963"
     CDS             26916..>27452
                     /locus_tag="KPD58_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG0264746.1"
                     /translation="MIKTHRVKLNLTRTQFELVREKQMESANCWNYIVNLSKEYYFEH
                     KQWIKKNDIQKLIKGKYNLHSQTIQAISDKFDANRKTISELRKKGNTKAKYPYKTKKF
                     YIIPFKASAINRNAKGNLKLSMSKGRYLELDFNVENIKTAEIVWRNGYYLYYTFDNEL
                     NGVVAKGTNTVGVDLGEIH"
BASE COUNT        10431 a         3062 c         4743 g         9216 t
ORIGIN      
        1 taagtttaag tgggtataaa cttatagaaa ttcatttttt atttaaggta aaaatttaaa
       61 gtaaaatatt attttacata ataataacct tataaatttt gaaattactt atctttgtgt
      121 taaaataagt aaaatgcaca tattagagct aagtttttaa gtaaataaac aaagtgaaaa
      181 tataaatatt aaggagattt ggtatatgaa tgtttatgaa aaaggtaatg gagttatact
      241 agaaggagta acagattttg acccagtaca tatatttgag tgtggtcaat gttttagatg
      301 gcacaaacaa gaagatggct catacactgg agttgcaaag gggagaatat taaatgtcaa
      361 aaaagagaat gataaaatat atttaaataa tactaattta aaggagttta acagcatttg
      421 gtataattac tttgatttag gaactgatta tacagaaata aagaataagc taaaaaatat
      481 ggatgaatat ttaaataagg ctactgaatt tggatgggga ataagaatat taagacaaga
      541 tggatgggaa atgcttatat catttataat ttcctctaat aataggattc ctatgataca
      601 aagagctata gaaaatttat caagaaagtt tggtaagtat ataggtgaat atgaaggcaa
      661 tgaatactat gcattcccaa caccagaaga actaaataag gcctcacaag aggaaataag
      721 agcatgtcaa acaggtttta gagataagta cataaaaagt actacacagg cagttataga
      781 gaataatgat gaggtatctg aatatacaaa tttaagtaca gaggattgta gaaaagagct
      841 tttaaaattt aatggagtag gaccaaaagt atgtgattgt attgcattat ttggtatgca
      901 aaaatatgat tcttttccag ttgatgtttg ggttaagaga gttatgcaag aattttatat
      961 agatgaagac atgagtttac ctaaaatgag aacctatgga atcgataaat ttaaagaaat
     1021 gtctggattt gctcaacagt acttatttta ctatgcaaga gaactcggta taggaaaata
     1081 aattaagggg gagaaattat gggagtaata ggaacaatat ttctgttcta tttaattata
     1141 tcatatctag ctggagccat aatttctgtg attatattac ttgaaaatag agaccctgca
     1201 aaaacaatgt cttggctatt gatgtttatt atatttccag gtgtaggttt aatgatatat
     1261 gctatttcag gtagaaatat tagaaaaaga aaattattta agactcaaaa actagctaat
     1321 aatataaaag aaaagaaact atttgataca ttagaaaaga taacagaaat agttgaatta
     1381 gaaaaagaat ctataaaaca aaataagctt ttaagagatg aagaggatgg tagttataga
     1441 aaaagggtaa taaatatgtt gttaaagact ggtatgtttc cctttactaa aaataataaa
     1501 gtggatgtat ttgtagatgg aaatgaaaag tttaaaagac ttatagaaga tataagagaa
     1561 gctaaagacc atattcattt agagtacttt atcatcaaag atagtgaaat aggtagggtg
     1621 ttaaaagaag aacttataaa aaaggctaaa gaaggcatta agataagaat tttatatgat
     1681 gatgtaggat gctggagatt ttggtttaat agaaaatttt tccgagaaat gagggaagta
     1741 ggtatagaaa ttgcagcatt tttacctaca aaatttccta taataggagg gaaattaaac
     1801 tatagaaatc acagaaaaat tgttgttatt gatggaataa ttggatatac aggtggaata
     1861 aatataggag atgaatattt aggcaaaaat gataagtttg gatattggag agatactcat
     1921 attaggatta agggtatttc tgtatacatg cttcaaatga catttctgat tgattggtat
     1981 tatacgacaa aagaagtatt agtgactaaa aattatttcc caagtgtagg gaatgttggt
     2041 gaaagtatga tacaagttgt tgcgagtggt ccagacagtg actgggaaga tattcattat
     2101 gcttattttt ctgcaatatg tcaggctaga aaaaatgttt atatagaaac tccatatttt
     2161 attccagatg aaagtctact aaaagctata aagagtgctg ctcttagtgg agttgatgtg
     2221 agaataatat ttcctaaaat tgcagaccac aagatagtaa atatagcatc ttattcatat
     2281 tttgaagaaa tattaagagc aggtggtaaa gtttatttat ataataaagg atttatacat
     2341 tctaaagtcg taataataga tgataaaatt gcttctgcag gtacagctaa tatggattta
     2401 agaagtttta tgcttaactt tgaggtaaat gcatttatct atgatgaaga agttattagg
     2461 gtaatgacag atgatttctt tgaggattta agttactgtg aagaacttaa tttggaggta
     2521 tttaagaata gaaatatcat acaaaaaatc aaagaatctg tagcaagatt attttcacca
     2581 atattataaa aaattattaa aaaaggtatt gaaaattatg tgtgattggt atattataat
     2641 aattgttagc actctattag atagagtgat aataaataaa aagtatatat ataaatctac
     2701 atatataagt agtattaatt taaaaataac taaaaacata ggcgaactag tctatactta
     2761 atattttagg aggcgtgaat aatgaaaata agaccattag ctgacagagt agtaattaaa
     2821 aaagtagaag cagaagaaaa aactgcaagt ggaatagttt taccaggagc agctaaagag
     2881 caacctcaaa tagctgaagt tgtagaagtt ggaccaggtg gaatagttga agggaaagaa
     2941 ataaaaatgg aattgacagt gggagataag gttatattcc aaaaatattc tggaacagaa
     3001 gttaagatag aaggacaaga atacacaata ctaagacaga gtgatgtatt agctgttatt
     3061 gaataaatat agaataaatt tattaggagg ggtttaaaat ggctaaagaa attaaatttt
     3121 cagaggaaac aagaagagct ttagaagctg gtgtaaataa attagcagat acagtaaaag
     3181 taacattagg acctaaagga agaaatgtta tattagataa aaaatttggt tctccactta
     3241 taactaatga tggagtaact atagcaaaag agatagaact agaagataga tttgaaaata
     3301 tgggtgctca attagttaaa gaagtagcaa ctaaaactaa tgatgtagct ggagatggta
     3361 ctacaactgc tacagtttta gcacaagcta taataagaga aggtttaaag aatgtaacag
     3421 caggggctaa cccaatactt ttaagaaaag gaatacaaaa agcagtaaca gtagcagtag
     3481 aagaattaaa aaatcaatca agaatagtag aaacacaaga ggctatatct caagttgctt
     3541 ctatatctgc tggtgatgaa gaagttggaa aattaatagc agaagctatg gaaatagtag
     3601 gtaaagatgg agttataact gttgaagagt ctcaaactat gaatactgaa ttagatgctg
     3661 ttgaaggtat gcagtttgat agaggatttg tttctgcata tatggttaca gatgtagata
     3721 aaatggaagc agttttaaat gacccatata tattaattac tgataaaaaa atatctaaca
     3781 tacaagaatt attaccagtt cttgaacaaa tagttcaaca aggtaaaaag ttattgataa
     3841 tagctgaaga tgtagaaggt gaagcattat ctacattagt agtaaataaa ttaagaggaa
     3901 catttgatgt agtagcagtt aaagctccag gctttggaga tagaagaaaa gaaatgcttc
     3961 aagatatagc aatacttaca ggtgctcaag taatatcaga agaattaggt tatgatttaa
     4021 aagaagctga tttatctatg ctaggtagag cttcatctgt taaggtaact aaagaaagta
     4081 ctacaatagt agatggttct ggagataaaa aagctataga agatagagta actcaaatta
     4141 aacatcaagt agagcaaact acttcagatt ttgatagaga gaaattaatg gaaagattag
     4201 ctaaacttgc tggaggagta gctgttgtaa aagttggagc tgctacagaa gtagagttaa
     4261 aagaaagaaa gttaagaata gaagatgctc ttaatgcaac tagagcagct gtagaagaag
     4321 gaatagtagc tggtggagga actgcttttg ttagtgttat accagcaata ggaacactaa
     4381 ttgagagttt agaaggagaa gttaaactag gtgctcaaat agttaaaaaa gcgttagaag
     4441 agccattaag acaaatagct ataaatgctg gtcttgaagg tgctgtaatt gtgcaaaatg
     4501 ttgttaattc tgaagcagaa actggatttg acgcattgaa tgaaaaatat gtaaatatga
     4561 tagaagctgg tatagttgac ccaactaaag ttagtagaag tgctttacaa aatgctgcat
     4621 caatagcaag tactttctta actactgaag ctgctgttgc tgaccttcct gaaaaagaag
     4681 atgcaggaat gccaggaatg ggtggcggaa tgcctggtat gatgtaattt aaaaaattta
     4741 aaatataaat tttaatataa agaactattt cgacttagat tttaaactct aagttgaaat
     4801 agttcttttg tatatatcta atttttgtat atctataaat tcaatacaca tatttagata
     4861 atcagtatct agtttgtaat aatattttag agtagtattg ttatttttta ggttatgatt
     4921 ttagatatat attaattaag gtataattgt tgatagagaa taaatcaaat ttaaaaggat
     4981 gcgataatat ggacttaaga aaattgcttg aagaagttaa aaatgaaaat attgatatag
     5041 attttgcgtt agaaaagtta aaagatttac cttatgaaga ccttggatat gcaaatatag
     5101 atcatcatag agaactaaga aatgggtatc cggaagttat ttattgtgaa ggtaagagtg
     5161 atgagcatat tttagggatt attgataaaa tgaatcaaaa aagttctaac atattgggaa
     5221 caaggtgcag aaaagaaact tttctgaaaa ttaaagaaat ctataatcat gctgagtatg
     5281 aagaggcgtc taaaattctt aagattcaaa atcatgatat agagaatata ggaaaaggta
     5341 aaattgtgat tgctacaggg ggaacttctg atataccagt agcagacgaa gcttatcata
     5401 cagctaagtt tttaggtaat gatgttgata gaatatatga tgttggagtg gcaggtatac
     5461 ataggctttt aaataaaaga cataaaatag atagtgctag agtaatagta gccgttgcag
     5521 gtatggaagg tgctcttgca agtgtaattg gtggactggt tgatgttcca gtaatagctg
     5581 ttccaacttc tgttggatat ggagcaaatt ttggaggatt agcagcatta ctagctatgt
     5641 taaatagctg tgcatctgga atatctgttg taaatattga taatggattt ggagcaggat
     5701 atttagcatc aatgataaat aaactgtaaa tatattgata taaattaaac aaaactatat
     5761 aataaaattt aatttctatt atatttttac tattaaaatt gaggagacat atatgagtga
     5821 aaaaatactt tattttgata taataagcgg tatatcaggg gatatgaccc ttgcaagttt
     5881 attaaattta ggtgtgccta aggaaatttt tttggaggaa attcataaac taagaatgag
     5941 cgatgaattt aatataaaca tcagctcgaa aactgaaaat ggaatagtag gtacaaaagt
     6001 agaagtaatt acaaaagaaa aacatacaca tagaaattta gtggatatat ttgaggttat
     6061 agataaaagc actctaaacg aaaatgtaaa atctaaagca aaaaaaatat ttatggtaat
     6121 tggagaagca gaagcaaaag ttcatggaac tacaatagac aagatacatt ttcatgaggt
     6181 aggggctata gattcaattg tagatatagt aggagcttct atattagtcg acttactttg
     6241 tgtagataaa gtttgtgcta gtacagtgtc tgttggttca ggatttgtaa aatgtgaaca
     6301 tggaattata cctatacctg cacctgcaac tatagaaatt ttaaaaggtg tgccaattaa
     6361 attaaataat gtaaatggag aatgtacaac tccaacagga gctgcaatca taaaagtttt
     6421 gtgtgatgag tttgtagatg agcttgaatt ttctccaaag caaattggat atggtatagg
     6481 tcataagaag tttgaagtac caaatatact cagaactgta ttaggtgaaa aaaaaaagaa
     6541 gaattaattt atgaaattag tgcaaacatt gatgatatgt cttcagaaat ctattcttat
     6601 ttgtatgaaa aaatattaga tgagaaagca ttagatattt atacagagag catttatatg
     6661 aagaagaata gaccagcaac aaaactatca attttatgta atgaatcaga tttagaaaaa
     6721 tttattaaaa tattactttt agaaactagt acttttggag tgagatataa tgcatataat
     6781 agagaaattc tagagagaaa atttgtaaaa ttagatacaa aatatggagt ggttacagta
     6841 aaactagggt attataaagg aaaactagtc aaggctactc cagaatataa tgaatgtaaa
     6901 actatagcaa aaaatgaaga tattcctata aaaaaagtgt ttgctgatat aaattttaga
     6961 ataaaagaag aatttagtga aaacttattg acataagtaa taaattttga taaattaata
     7021 ttcagcctta ttaaataaaa gtaaacagaa aaaaatatat aaaatataaa ctcactcgta
     7081 tatgctcaga aatatggtct gagagtctct accaagatac cgtaaatatc ttgactatga
     7141 gtgaaattat tataccaaaa gacctagtta agtaaatgtg taggctgagt taaactccct
     7201 atgtgttggc tataagataa aggtattttg gaagcctgta tagtaatttc acttttgcta
     7261 gaaattatgg aagttactat acaggttttt tgtgtaaaaa gatttaatat aatttagact
     7321 gtattgtgtt aaaataaata tataaaaact atattataca aggagagatg taaatttatg
     7381 aaacatgaat tagtacttgt aatagatttt ggaggacaat ataatcagtt gatagctaga
     7441 agagttagag aaaacaacgt atattgtgaa atacttccat gtactgcaag catagaaaga
     7501 ataaaagaaa aaaatcctaa agggataata tttacaggcg gtccaaatag tgcgtattta
     7561 gaagattctc caacaatatc aaaagaaata tttgaactag gagtaccaat acttggaata
     7621 tgctatggta ttcaaataat gtctcatgta ttgggtggag ttgtaagaaa aggtaataaa
     7681 caagaaaaag aatatggaaa aacagctata acttatggaa aatcatcatt atttgaagga
     7741 ataactacaa atagtgtatg gatgagtcat acagatttaa tagaaaaagt tccagaagga
     7801 tttactatag ttgcaaatac taatgattgt ccagtagcag ctatggaaaa tgtagaacga
     7861 aacctatatg gagttcaatt ccatccagag gtagaacact gtttggaagg ggacaagata
     7921 cttacaaact tcttatataa tatttgtaaa gtaaaaggtg actggactac agattcattc
     7981 atagaagata aaataaaaga attaaaagaa aaaataggcg ataaaaaagc tttatgtgct
     8041 ttaagtggag gagttgattc ttctgttgct gcagttctta ttcataaagc aattggggac
     8101 aacttaactt gtatatttgt tgaccatggt ttacttagaa aaaatgaagg aaatgatgta
     8161 gaaagaatat ttagagagaa atttgatata aatttaataa gagttaatgc agaagataga
     8221 ttcttatcta aattaaaagg agtttctgaa ccagaagcta agagaaaaat aataggtgaa
     8281 gaatttataa gagtatttga agaagaatca aataaattag gaaaaatgga tttcttagta
     8341 caaggaacaa tctatccaga tgttatagaa agtggacatg gaaatgctgc tactataaag
     8401 tctcatcata atgttggtgg aatcccagaa gatgtagact tccaagaaat tgtagaacca
     8461 ttaagagagt tatttaagga tgaagttaga aaaataggat tagagcttgg tatagaagaa
     8521 ggattaatat ttagacatcc tttcccagga ccaggtctag gaataagagt tataggtgat
     8581 gtaactaaag aaaaatgtga tatattaaga gaagctgatg ctgtttatat ggacgagctt
     8641 agaaaagcag gtttatatag agaaatatgg caagcttttg caacattacc agatgttaag
     8701 actgttggag ttatgggtga tgaaagaaca tatgcttatt tagttggttt aagagcagtt
     8761 acttcttctg atggaatgac ttctgactgg tataagatgc cttatgatgt attagaaaga
     8821 atttctaata gaataataaa tgaagttgat ggagtaaata gagtagttta tgatataact
     8881 tctaagcctc ctggaactat tgaatgggaa taaaatatag aatgccaaaa atccacataa
     8941 atacaggatt taaggtctct cttacttcta tgttatactg gtttgatact atcaatattt
     9001 gccagtatct tatggattaa taagcaaaaa acatcctgct gaatgataaa ttcagcgggg
     9061 tgtttttatt ttgttttaga actcctttgc tctgaaactc gaagtattta acactgatga
     9121 ttttgttatc ttttagttcc ttttgtgtca tgctctgaaa gacaaattga tacaataaaa
     9181 aagtaatttc ttaatcattg atggagatat gtatttatgt tactcttgat ggtaatttga
     9241 gtaatattgt tcatagcttt ttatattcac ttaattagag aatcatttgg tataatcata
     9301 aataaacttg atgatataat agaaaagatt tataattatt agaagtatat aaaagtattt
     9361 tatgtctatg aaatgtatgt ctataaaaca tatagatgac ttttgtatat taatgtatga
     9421 atattgatat tatagaagaa ttgagttgtc aatgatacta aatcaaatat ttatagaagc
     9481 tggagggatt atgagaaata aaataattat aggaattact ttttcacttg tattactaat
     9541 gtctggatgt gcttcaaaac aaacagcaga aataaacgta ttagcaacta ctgatttaca
     9601 tggagttata ccttatgagt taacttctta tgtaaaagaa gaaagaaaaa aagataaaaa
     9661 tctaacttta gtagatgcag gagatttttt tgatagtgga gaagtgtttg gttctagcat
     9721 ggatagttat tttagtgcta ggtataagga tattgataat aacactgagc gttacattga
     9781 gacacctatt tcaaaagata tgaaagaggt aggttatgat gttgcagttc ttgggaatca
     9841 tgaatttata tccaacaata aattttatct agataatatg atatctgatt ttgagaagca
     9901 aaaaataaat attttatcag caaatacgta taaaaaaaat ggagacagtt atacaaaacc
     9961 atacataata aaaaatatta atacacctga aggaaacgta aaactaggaa ttttagggct
    10021 tactataaaa gaagttggtg agaggaaaca gtggaaagat ggaaaacttg ttgactctaa
    10081 gtctttagaa ctaaaagacc aagagggcta caatggtgaa ttgtatatga atgatttagt
    10141 agaagatgct aaaaagtggg ttagtataat ggaaaaagag aagcctgaca ttatagttgc
    10201 tgtggcacat acaggtgaga aacctaagaa acctagaaac ccaggaaata gaatacaaga
    10261 cttagctcaa caagttgatg gaattgatgc tatagttgca ggtcataatc atgttcaaat
    10321 tgagcaacat gattataaaa ataaatcagg agaaaatgtt atagttactg agccaggaaa
    10381 gcatggagaa tgtatttcta agattaattt taaattagaa aaaaacaatg ataaatggaa
    10441 tatagttgac aaatctagta aattaattaa atttgaaaaa aatcaagaaa gtgataaatt
    10501 tggagaattt atttcttcaa ttaataaagt aagtaaagat aagaaagaaa taagattgtc
    10561 agaagtaaag actcctgaat gggataaaat atatgttttt aaatctggaa catctgcaga
    10621 tgatatatat aaaacaatag gatataaatg gagaaatata tcatctccaa agggtgatga
    10681 catggtgcag attgttgtaa tgaataataa taagcctgtt agctatgtat atggtgaagc
    10741 tgaagttatg ccaattaata taaaatgtga tgaatcagag tataaagata atgttgtaac
    10801 tatttatcct aataaaaatg ataaatttaa aattgaaaaa ggtaaacaag aatatagtat
    10861 taatttaaaa catatacaat agaaactttt atcttattta agtttagatt gtacttaaaa
    10921 taaagtgtgt attattttaa aaattgatga taattaaaat cacaacatta aggaatagta
    10981 cacacttttt atgctaaaaa atagaatgtt aaaaaattaa acctttaaat taaagtacat
    11041 tgcttatatt atccaatatt atcttcaaag gttgtgtgat atttttatcc ttatgtaata
    11101 ttgctaacat tttattttcc atatctatcc catctaattc aatcaatcgc aattttttat
    11161 gtaagagtga atctaataat aaattttctg gcaaaatggt tatacctaac cctgcttctg
    11221 cagctttaat aagtgcaaaa gaattaacac tttcccaaac aggataagct ttttggtttg
    11281 aaagagataa tacactgtcc aaagtattgc gaatggcact tccttgctca cgtaacaata
    11341 acggaaacgt gcataactgt cgtagagata aggcttgttt agtattggtg gtggttatgc
    11401 aatcatacct ttaatccaag agaaagtagt aaatcaaact ggatggataa gtgagaaaat
    11461 gtttacagat attataacta tttcacaaat gacaccaggg ccactggctg ttaatacttc
    11521 tacttttgtt ggacttcaaa ttggagggtt ttggggagca tttgcagcca ctttaggttg
    11581 tgtattatgt ggtgtagtaa tagctctatt tttatataat ttttttcaaa agcatcagaa
    11641 ctctacttat atatttgaaa tattaaatgg tctaaaatca gcttcattag gtttaattat
    11701 ctcagctgcg gctacaatta taattcttgc attatttggg acgaataaag tgtacttcaa
    11761 ttctatattt gagatgctaa actggactgc actaataatc ttttttgtta cactactgat
    11821 agtacgtaag tggaaaatta atcctattgt gattataata ataagtggta tactgggaat
    11881 atttttttat ttataagttt ttgctaagta aaggatatct atttttttat atcttaaatc
    11941 caaatagcca ataaagtaag gctatttgga tttttaataa attctgaaaa aataatcaga
    12001 aacccatata acatttaaat tatatcactg gttttctaca aaccaatcat aagcttcttg
    12061 gcaaaatttc tcactatcta tatagtaact attatgaaca ttattaattt caacaagttt
    12121 ctttttacat tttagtgaat tataatatct atcaaaatat tgggttttaa aatatctatc
    12181 atgaagtgtc ttaaagacaa gagtaggtat gttgactcca tcttttatct tactatcttc
    12241 tgcaataaag cattctaaca ttgtattaat tatctttgta ctatttttcc aaatatgagg
    12301 atttttcttt tcaaatattt tataaaattc attatcatag acttcattat aacctttata
    12361 tttaggtggt tttactgtta tgtgaaaatt aggaagaaac ttaaagaaaa agtttaataa
    12421 tttaacagtg agataggtat agcctactcc tgttgtgttt ttataatcaa cacaagcaaa
    12481 ctgagcaaaa ctttttaact tattgtctga acatgcataa tattgtgcaa acataccacc
    12541 aattcccgta ttcccatata gatgtatgtt ggaagaataa tttttttcta tgtatgtaat
    12601 gacagaatct aagtctttta caattgaatt gcgagaaaaa tctttttcat atccatcact
    12661 tttccctgtt cctgaaaaat caaaagcaaa tatatttaca ttatgtttgg caagtggttc
    12721 atatgctttt tgcatctcaa gcgtagtgac aataggagta tgtgtaacaa caatattcat
    12781 ggatgatttt ctggtaataa atgagttaca aaaaatcatt gtatctccat ttggtatttt
    12841 tatatgtttc caactcattt gattttcctt tctctattat aaaatataat aatttttatg
    12901 ttctgtttta tatattattc atattttatc acagataata tactaaagtt aattttttta
    12961 ttgtacacct attataataa ttcttctata atataagaca ttctataatt ttatatttaa
    13021 gttcatgtgt atttaattgt ttttatataa aatgtccagt aatactattt ggatttattt
    13081 ttatttcatc aggagttcca gaagcaacaa tttcccctcc atttattcca ctgtctggac
    13141 ctatatcgat aatccaatca gaagcacgta taacctctgt attgtgttca ataattaaaa
    13201 gtgaatgctg ttcatttaca agttttctaa aagtaattaa caaattttct atatctttaa
    13261 aatgaagtcc agtagtaggc tcatccaata tgtacagcat tcgtttttta gtttttgtcc
    13321 ctaattcttt tgctaatttt atcctttgag attctcctcc acttaatcct aatgtatttt
    13381 gtccaagctt tagatatcca agtcccactt catctaataa ggagagtttt ctatatatat
    13441 cagagcaatc gctaaaaaat gtttttgctt ctgctacttc catgtcaagt atatctgcaa
    13501 ttgtctttcc cttgtattgt atagacagta tttctttttg atatctctta ccttgacatt
    13561 cactacaagt tacccattgg tctgccatat attgcagaat aatttttgtt ttacctaatc
    13621 cttgacagtt tgggcattgc ccctttttgc tattaaagct aaaatagctt tcatccaata
    13681 atttttgttt tgcttgtgga gtttcagcaa atagagaacg aatttcatca aaaatattga
    13741 tatatgtagc aggtgtagaa cggctagatt ttccaatagg tgtttggtcc attaagataa
    13801 aatcatcaat agcatcaaat ccagtaaatt cagtataatt tttatctggg atagattttt
    13861 gtttcaattt ttcttctaga gcaggaagta aactgtgaaa tacaagagag ctcttaccag
    13921 aaccactaac acctgtaata gagcacatac agtttaaggg aatttctaca tcaatatttt
    13981 ttaagttatt tgcatggcat ccttttaagc ttaaccattt acttggtata gttttatttt
    14041 gtgaacctgt tttattgtat gtacttaaat atttacctgt tatagagttt ggatttttta
    14101 taatttcttg ggttgtacct tctgcaataa tgaacccccc ttttgtacca gcacttgggc
    14161 ctacatcaat aatataatca gcagacagta ttgtatcttt atcgtgttcg acaactataa
    14221 cagtattacc cttatctctt agctcttcta tcattttgat gattaaatta tggtctcttg
    14281 ggtgtaaacc aatagatggt tcatctaaaa tgtaggttaa gcctactaat tcacttccta
    14341 gctgacttga taatcgtact ctctgcagtt ctcctccaga taaagttgga gatgttctat
    14401 ctgttgatat atagctaaga cctaccttta atagataact aactcgaatt tttaattctg
    14461 ttaagatatc atttaccaaa gagagctcat ttttttgaag ttgatttggt aattcacaca
    14521 accaattcca taactgttct attgtcattt ttgttagttc agggaagcga taacctttga
    14581 ttgtagtgaa tctagcctca atgcacagca attcgccacc acatgtattg caaggaattt
    14641 tgttcatata ttgatgcaag gtattatttt cagaactaga ttctctaaaa agtctttgaa
    14701 tgtggttgat tgctcctgaa gcaggtcttc taatctttga ttctcgtccc tttgattcaa
    14761 agctaaattc atatatctta tcgtctgtac catataagat tgcatctata aaatctcttg
    14821 gtaattcatt ccaaggtata tctaggtcta tatttttatc ttttgctatt gcatataatt
    14881 cacctaccat ccaattacca tttggctttt tccccctcaa ttttccaaaa tagtaagtgg
    14941 ctccatctaa tattgaaata gttgggtctt ttacaatcaa ttgaggatta acttgatagg
    15001 taaaacctaa tccattacaa tcgtgacaag ctccagtatg ggagttagca ttgaaggttg
    15061 aagtatttat cttagaaaaa atagtttgac aatgcggaca ataatttttt gtattattct
    15121 ttttttctaa tggaatttta caatatggac agtaactttt tccgattgat acaaataaca
    15181 ttcttaaata atcccaaatg cttgtaagag taccaacagt agagcgagga ttttggcttc
    15241 cttttttctg ttcgatagct atagtagggg ttaatccctg aagtaaatca aattccggtt
    15301 tttgaagctg ttctgaaagt tttgctccat tacttagatt ttcaaaataa cgtctttttc
    15361 cttcttcata aatcgtatcg aaagctaaag aagattttcc actaccactt acacctgtaa
    15421 taacagttat ttgatttttt ggaattgtaa cctcaacatc ctttaaattg ttttgccttc
    15481 ctccttttaa atagatagag cttttattat gacaaagtga atttttaatt gatttattta
    15541 ttgattttgt aaatttgaat ttcttttcta gttcaagagg attgcctaat tcttttattt
    15601 ccgaaatacc taatattttt aactgagaaa agattgattt caaataatct tcaggcaatt
    15661 tttctattaa caaattaagg tcttctattt ttatatttcc gcttgataat atttctgcca
    15721 tttcatctaa taaatttttt tctgctcttg ttaattgatt gtattttttg gaacttaatt
    15781 ctgtccataa atcccatgga agcaattcta aaccaatcat attattaaag tcatgtagaa
    15841 gattaccctt tacagaatgc caacctttcc aatttttatt atatccaaag tcatctggat
    15901 gagcatgtcc ttgccgacat cttatccagg cttctgaacc agttaaaaaa ccatctgttt
    15961 ttccgacatc atgagtgtca aaattaattt gaaaataatt tttttgtatg tcatctattt
    16021 gtgcatctat tcttatccat ctctttgtta gagtatcata gtattctaca agccagtggt
    16081 cttcataagt aagctcactt tcaaaataat tagcaaaacc tgctctcatt ctagcctcaa
    16141 aacctcgata tcttaggagc gatacaagaa gtaaagaata atctctgcac attccaacaa
    16201 gcctttgttt tgggtttctt ggaatagtta gtgggttaga atttatttca aatattcttt
    16261 ttaacatttg aggaattgta cgaagcaatt cctcatcaga ttgttgctta ttaaaggata
    16321 taccatataa ttttccttgg tctccatgaa ttactaggct ttgaaccact tttaataact
    16381 caattggagt ctttggtaaa tcttgatatt tattttcaaa ttttttagga tttgtgatga
    16441 tgctttgatt tttatagtta tttattttca tttatatttc acctctctaa atggatgata
    16501 taccctgaca tggtgtcata tgcaaggaca atttaaattt tttgtccaaa atttaaaaat
    16561 aaataaaaaa tgatacagtg ataaattgta atacaaatat agtttcaaat tgcatataat
    16621 ataattattt aataaaacca ttatctatgg aatgttaatg aaatgttaag atggcattct
    16681 tgaatattaa gaaatagaat actataatat agtgtgaagg attcttagta aaaaaatttg
    16741 taagtgttaa tatacattaa aaaataggtt tataatttgg aggaaaaaaa tgaaattatc
    16801 aaggtataag aaaaatagtt tagttctgtc tataatttta tgttcactta ttatttcaat
    16861 ccttgtaatt actgttttta ataccgtaaa actacacaat atatctaata aacaatctaa
    16921 aaactattta aatgatgtat ctacacagat tgtaatgaat gttgattcta aaatcaaatt
    16981 tattttatcc gacttaagaa taatggcaga ttttattaag caatatgaag gtgatagtcg
    17041 atatgagtat ttatctaaaa gaaagactag ttataagtat tatgatgttg gaataataga
    17101 tttagaaggt aaagctgagt ttttaagtgg aaaaaagttt gacttaaaga atactgcttc
    17161 ctataaaaaa ggaattgaag gtaaagaata ctcagaagta atagagtcaa tgggttttgt
    17221 tctttatagt gtacccatat ttaatgataa tcatgaagta tctagtattt taataggtgt
    17281 atccaataaa gaaagtatga atgagattct taatatagat aactttaatg gagaaggaac
    17341 tattgaaatt attaatagta agggaaaacc tcttttcata ggaaaaaata ctgaattaat
    17401 aaaagacctt agccataaat ataaggattc tacagatgaa ccttgggcac aaaagatgct
    17461 taatgatttt aaaaatgata aatcagggaa tattacaata acctcatcaa aaggtgtaca
    17521 atgcttattg acttatcatc caattacaag aggtttaaat gattggcatt ttcttctcat
    17581 agtacctgaa gatgctgtac ttggtgaact taataagtta aattctttta caataacaat
    17641 gacatttata attacatgta ttattggaat tataacatta attttatata ttataagaag
    17701 aaaatatgta aatcaaatag aaaatattgc atatttagat agtataacaa atggcataaa
    17761 ttcaactaag tttagtatgc tagtcaaacc tttaatttca gaatcacctg atagtactta
    17821 tatgatgata gcaatgaata taaaagattt taagctaata aatgattgtt tcagtagtga
    17881 aaagggaaat atgacactaa aacatcttta caatattttg tataaaaaca ttgatgaaaa
    17941 tgaagaattt gtgtgccgtc atgatgcaga ccttttctat ttgcttatta agaatagacc
    18001 agtgttagaa gccctggagt tattatataa aatagaagct gacacaaatt attttaataa
    18061 aaatagggaa aatccatact ttttaagact atcagtaggt atttacacaa ttgaaaatca
    18121 taatgaagat ttaattacaa tacaagacca tgcaaataca gcaagaaaaa gctttaataa
    18181 aactcatcaa agtgattttt ctttttatag tgatatagaa agaaaacgat taatatctga
    18241 aaaggaaatt tcaaatttaa tggaaaaagc ccttgaaaat aaagaatttt ttatgtgctt
    18301 acaacccaaa attgatataa aaagtggaaa aattagtgga gcagagtctc ttgtgcgttg
    18361 gaaaaattca gaaaagggca taatttatcc aagtgatttt attcctcttt tcgaaaaaag
    18421 tggatttata tgtaagttag atttatatat actagaagaa acatgcaaat tgatttctaa
    18481 gtggataaaa gaaggtaaag aagtgttgag aatttctgtt aatgtatcac gtcaacatct
    18541 gaatgataaa ttgtttttag aaaaatataa gcatatatgc aataaataca atgtaccaac
    18601 atgtctgata gatttagaac ttaccgaatc tattttctta gaaaatccag aagctataga
    18661 tataatagag gatattcatt ctaatggatt taaatgttct atagacgatt ttggatttgg
    18721 gtactcatca ttaggaatat taaaggattt taaagttgat ataattaagt tagaccgttc
    18781 attctttgtt agcaaaaata atatagaccg tggtaaggta gttattaagt ctattattga
    18841 acttagcaaa aggctaggta tgaaagtaac tgctgaagga attgaagaat tagaacaagt
    18901 tgagtttcta aaaaatgtgg gatgtgatta tattcaaggt tatgtttttt caaaaccatt
    18961 attaatttct gattttgaaa attttgcata tgaaaataat aaaattaaaa atatccatta
    19021 ttaaatgtac aagataaaaa atttaggcaa gttatataat taaaaatata acttgtcttt
    19081 tttatttata tatttaataa aaatatattt gcagattttg taaaaataat tttcaaatgt
    19141 tgtaagttgt ggctaaagct ttattgtatt attctgaata tagaaagata gttactgtct
    19201 tagataaaaa ctgaaacata cataataata taaaatttgc tatattaata actctaatta
    19261 ttttggaagg agggaaaata tgataagtgg gagaatgtta ctcatcatga attttctaaa
    19321 aggaaaaaat aagacaagtt acaaggatat tagcaaacaa cttagtatag aagaaagaaa
    19381 agttagatat gatataaata atttaaatat tgttttgcgt tcattaaaca agtcagtaat
    19441 acaaaagatg aacaaaggtg tgttaattat tcctgatgac ttccaagtag tatgtataaa
    19501 taataacgaa tatgtatttt ccttaactga gcgagtatct attatgaaaa ttatatcaat
    19561 gtttaaaata gataatctaa acttagaaaa attaagtaaa gaatttcagg tttctagaag
    19621 ttcaattaaa aatgacctta atgttttgtc aagtgaatta gaaagaaacc aaataactat
    19681 tacttatgat aaatctttta aaataagtgg agaagaagac attatatata agcaaagatt
    19741 gaacatatta aaatcgtatt cttatctttt tggtaaggaa aaatctgatt taagtgtatt
    19801 tgagaagtat ttagtgaatg taattgaaaa aatattcaaa gggatttatt tgattgatat
    19861 atataattgg tctatgaatt tattattgca aatgggatgg actttaaatg atgaatcttt
    19921 tacatggtat gtgtcaaata ttttcctttt tacatggtat atacattcag atgaaaaaca
    19981 tcctttagaa tctgcttcat taagtgagcc gtgttttgaa aataagtttg accaagaatt
    20041 tgaaacaatt attcaaaaaa agttgactaa ggaacaaatt tttctgttaa agagttttgt
    20101 attttttaca aataaatatg caagtttaaa cgaggatatg gatttgatta gtacagaaat
    20161 tatagttcaa gatctaattt acaatatgtc agaaatatta gagattagct ttaaagaaga
    20221 tatgattctt tataaaggat tactcaatca tattgcccca ttgatagaga gaatcaaaca
    20281 gaatatacag atttatgagg aattacttca tgtaatacca gataaatatc aatatgtact
    20341 tgaagctact aaggtttcta ttcaaaaaaa cagagtttta agtcaaataa agaatgaaaa
    20401 tgaaacaata cttttggcaa tacatttctt aggaagtata catagaaatg agaggaataa
    20461 ctacaaaaat atattgttag tttgtggatt tggatatggt gtaattgcta tgttaaagga
    20521 tagtttagta aatgattacc agataaatat agtagagagt attccatcat ataagttaca
    20581 agattataaa gaatgggaaa gtattgactt cattataaca acatcaaaga tagtaataga
    20641 cttgcctaaa acaattattg agataaatcc atttttacaa gaagaggatt atttgaagtt
    20701 tgaagaaaaa gggataaaaa gaaagaacgt cttaactaat tatatgtcaa taaaaaaaag
    20761 gttggacttt ttagaagaag atgcccaaaa aagagtatta agtattatat gtcaagaact
    20821 tggatacagt gatgagagaa tattatttag acaacttaat ttaagtgatt taatagggat
    20881 tgataccata aggataattg atggagatat gaaatgggaa gatgctgtaa aatttagttc
    20941 aaacatactt gctaattgtg attttgtatc agcacaatat gctgagaata ttatagatat
    21001 tttaaaaaat gtgggttttt atgctgtaaa agataacgaa tttgctttac tacatggcaa
    21061 tgatagttca ttagtcaaag taagttcgat aagtttatta atatgtaaag aagcagttag
    21121 ttttggagat aaaaaggtta aaatcatatt ttgcctagca agcagagata aaaaagagca
    21181 aattcctgca ataattaatc taactaaaat ggtttataag actaatttta ttgctatgtt
    21241 agaaagtgca aagacaaaag aagaagcaga agttttaatt cataaatatg agaaggaggt
    21301 cactgtatga ataaattagt taatgtaaaa tgttcattta aaatacatgt taattcaaaa
    21361 gaagaagcca ttatatcgtt agttgatgta gtgtcaaaag aaggatattt aatagataaa
    21421 aatcaatttt taaaagatgt tttaaagaga gaagaaacat tgtcgactta tattggacat
    21481 ggtataggtc ttcctcatag tcaaagtgta ggtgttaaaa actcttgtat tacaatagga
    21541 aagcttgata ctcctattga atggacagaa gaaggagaaa aagtggattt aatatttttg
    21601 atatcagtaa caaaagacaa tgaaaataat cttcatttaa agattttatc aaaattagca
    21661 cgtttattga tgcatgaaag ttttagaaat caaattagat gttcagatga acaaactgtt
    21721 tataatctta taaaagaaaa aatagaggag gaagattaaa atgagaattt taaagttttt
    21781 gcaaaaacat cttatgacag caacatctta tatgattcca tttgtggttg caggaggaat
    21841 attatttgct ttatcagtaa ctctaagtgg acaagctgct gttcctgaaa caggctggtt
    21901 ggctaaactg aatcaaattg gagctgctgg cttggcttta ttcattccta tattaggtgg
    21961 atatatagca tttagtatgg cagataaacc aggattagct ccaggtatga taggtgcata
    22021 tttagcaaaa gaagtaaatg caggttttat aggtggtatc atagcaggat ttatagcagg
    22081 atttgttgtt ttacaattaa agaaaattaa attagctcct acaatgagaa ctttaggttc
    22141 tatatttatt tatcctttga ttggtacatt gattacaggt ggtataattg tatttttaat
    22201 tggagaacct atagcttctt ttatgacatg gatgacaaac tggttaaatg gaatgtctgg
    22261 tgtttctaaa attcctttag gtggaatctt aggaggaatg atagcctcag atatgggtgg
    22321 tcctataaat aaggttgctg ctacttttgc ccaaactcaa gttgacacac ttccatattt
    22381 aatgggtgga gttggcgttg caatttgtat acctccaatt ggtttaggat tagctacatt
    22441 attgtttcca aaaaaattta gcaaggaaga aagagattca ggtaaagctt cactattaat
    22501 gggatgtgtt ggaattacag aaggagctat tccatttgca acatcagatc cagttagagt
    22561 aataccttgt attatggctg ggtcgattat aggaaatatt atggcttttt tattaggatg
    22621 tttaaatcat gcaccctggg gaggcctgat agtattacct gtagttgaca ataggttggg
    22681 gtatattgca tcagtattaa ctggagcagt agttgttgca gtattaatga aattagtaaa
    22741 aaaagacgtg aaagaagatg aagaaattga agaagattta gatgaatcaa ttgaattaat
    22801 atttgaagaa ttataaatat aaatggaagg aatttgtata aaaaagtaaa tataaaacta
    22861 taaatttaaa ggagataaga atatgaaaat tgtagcagta acatcttgtc ctagtggagt
    22921 agcacatact tacatgtcgg ctgaatcact tgaattatca gccaaaaaat ttggtataga
    22981 aataaaggtt gagactcaag gaagttctgg aatagataat gaattgtcac ttaaagatat
    23041 tgatgaagca acttgtgtaa tattgactaa tgatgttgag attagaaaca tggaacgttt
    23101 taagggaaag aaggtagtaa gaatgagtgt atctgagata attaagaaat ctgatgcatt
    23161 aattaagaaa ataaaagata cttttcaata aaaattgatt tattttacat ataactcaaa
    23221 gatgttttta aagaaatttt tattaaaagc atctttgaaa atatcttaaa aatagaataa
    23281 aaggagatat agtcatgaaa aagttaccaa taaagactgt cgtacaaaga ttactaaact
    23341 tacaagaaga ggggaaaagt gcaactcttt taggaattgg tcctatgtca ccaaacttgt
    23401 tacaagcaag ttttgaacta gcaaaagatg atgattttcc tttaatgttt atcgcaagta
    23461 gaaatcaagt agatgcagat gaactaggtg gcggttatgt aaatggttgg aatcaggaaa
    23521 cttttacaaa agatattaag aaggttgccg acaaagttgg atttgatggt ttatattatt
    23581 tatgtagaga ccatggtggg ccatggcaaa gagataaaga gagaaatgac catttgcctg
    23641 ttgatgaagc aatggagtta ggaaaaaaat cttatttagc ggacattgaa gcaggttttg
    23701 acctattgat gattgaccct acaaaagacc catttgaaat tggaaaagta attccactag
    23761 atgtagtact agaaagaact gtagaattaa ttgagtattg tgaaaacgag agaaaaagac
    23821 ttaatctacc agacataggt tatgaggttg gtacagagga aaccaatgga ggtctgactt
    23881 caactgagac gtatgaaaca tttataacta gactaaaggt tgaacttgat aatagaggtt
    23941 tgccaatgcc aacatttata gttggtcaaa ctggtacttt aactagaaag acagaacaag
    24001 taggaatttt taattttaga aatgcttatg atttagctca aatggctaag aaatatggag
    24061 ttggtttaaa agagcataat ggcgattact tagatgatgt aactttatta gaacatatac
    24121 catctcaaat tattgctaca aatgtagctc ctcaatatgg tactgaagaa actagggcat
    24181 atttaaaatt agcagaagtt gagtgtaaat tagaaaaaga gggcttagtt gagaaaactt
    24241 ctaatatcag acatgttctt cttgttaatg caattgaatg tggacgttgg agaaaatggg
    24301 tagtaggaga acaaaaaaat ctaactactg aagagatatt taaggatgaa gttttatcta
    24361 atgaaatact agatattgca ggacattata catttaataa tgatgatgtt aaaaaagaaa
    24421 tagaagtgct ttatgataat ttaagtaaga ataatattga tggtcaaaga tttgttgtag
    24481 accatattaa acgtcctttg cgtgattatg cagaatgcta taacttaaaa ggtgttacta
    24541 cgaggatttt aaataaataa taggacttat agtggtgagt attgttatac taggtattta
    24601 tagttataat tatacttacc actattaaat ttaggaggac taatattttg attaatttaa
    24661 tttttgatgt agatgatact ttatataatc aattaacgcc tttttacact gcatataata
    24721 aagtgttttc ttcaataaag gatatttcta ttgaagattt atatatgagt agtagaaagt
    24781 atagtgatga ggtatttcat atgactgaaa atggagaaat gccaatcaaa gaaatgcata
    24841 tttataggat aatgaaagct tttgaggagt tgggaaattc tattacagaa aaagatgcac
    24901 aaagttttca agatgagtat atatatcaac aatcacaaat tacactaata ccagaagttg
    24961 agaggatttt aaatttttct aaagaaagaa atattaatct aggaattata actaatggcc
    25021 catcaaacca tcaaagaatg aagttaaagc agttaaatat tgaaaactgg gttgataaaa
    25081 gtaatatttt tatttctagt gaggttggat tttctaagcc agatactaat atttttagag
    25141 tagctgagaa tgttatgaat ttagatagag aaaatacata ttatgtgggt gattcttata
    25201 gaaatgatgt attaggtgct aaaaaagctg gttggaagag tatatggttg aaccatagag
    25261 gatatgaggt agaagaattg ttttataaac ctgattttgt aattttggaa cataaggatt
    25321 tgatatcttt atttataaaa atatgtagtt ataaaaatat gtagtaaaaa aatgttaata
    25381 atagaattgt ataaaataat taataataac tatataaaat tatatgttta gattgtgagc
    25441 aatctatttt atattaaaaa tagattattt agaagaaggg gaaatcttaa aaaaatttga
    25501 atatcccctt tttattattt aattaaaatg aaatagttag aaatatattt tatttagttt
    25561 agtcaataac attttgggat tctttataac tacatgttaa ctgttgaata ggtttatcat
    25621 atggtttaac ccatccttta ttaatactta attctgtaag tgctgtgtga ccttctacca
    25681 tttgagttaa tgaagctgaa tatatagctc ttaattcagg tgtactactt gtcaatgcag
    25741 aattaagata catattggca gatgctttag cagatgaaag catacttaac attattactt
    25801 tatcgtctat ataagtattg ttcttaatta gattttctat catatttctc atgttatcta
    25861 tacctcctca gaaattagta tttcattttc aactataaat tgttgtatag ctttaattct
    25921 tccttctgtt gctaatatac tagattctga ttgtcttttt aggtcttcat ctgatattaa
    25981 catatttatt gctctttgca taagtaatcc gtcagactcc atttttataa ttgctgctaa
    26041 agatattttt tcagtttctg aaagtattct catttaatca tctcctatat tgtaatttat
    26101 gctttaatag tatgtctttc ttttcaagga aataaccatt acactttttt acagtatttt
    26161 atattctctt tatatcaaaa tttagtcgac ctaatatatt aatataattt tgtaggatat
    26221 ttatcattaa atatctataa aataaattat ttgaatattc ataaagcaaa atttatttgt
    26281 atgtacttct tcaagagtca atatgagttt tttcaaaaaa ttaattttta tgataatcat
    26341 taagggctat atttttctta aagttgtgat tatcattatt aatttacaat aaaattgccg
    26401 aaaagccctc tcctttaggt gtagggatga aaggtaaggt cttaatataa aaaaagttag
    26461 aataatgctt gtaatctaat accgctactg ataccctata tttaggcggt gttagttttt
    26521 atgtataaat ctaacaaaaa tatagtttat gattgcaaat atcatattgt attttgccct
    26581 aagtatcgta aacctgtttt agttggcgaa gttgaaaaga tgttaaaaga aatactacca
    26641 tataaagcag atgaacttgg agcagaaata atagaaatgg aaactgataa agaacatgtg
    26701 cacttattaa tatcttgtga ccctcaatat ggaattcata aagtagtaaa aggtttaaaa
    26761 ggctttagtt cgagagtatt aagagagaac tttccacatt taaagtctag tatgcctagc
    26821 atgtggacta atagctattt tgtaggtact gttggttctg tgagtttaga agtagttaaa
    26881 caatatatag aaaatcaaca agttaggaca aagaaatgat aaaaactcat agagtaaaat
    26941 taaacctaac tagaacacaa tttgaattag taagggaaaa gcaaatggaa agtgctaact
    27001 gttggaacta catagttaac ttatctaaag aatactattt tgaacataaa caatggatta
    27061 aaaagaatga tatacaaaag ttaatcaaag gtaagtacaa tcttcatagt caaactattc
    27121 aagctatatc agataaattt gatgcaaata gaaagactat atcagaatta agaaaaaaag
    27181 gtaatactaa agcaaaatat ccatacaaga ctaagaaatt ttatataata ccttttaaag
    27241 ctagtgctat taatagaaat gctaaaggca acttaaaatt atcaatgtca aaaggcaggt
    27301 atttagaatt agattttaat gttgagaata taaaaactgc tgaaatagta tggcgaaatg
    27361 gatattattt atactacact tttgacaatg aattaaatgg agttgtagcc aaaggcacta
    27421 ataccgtagg tgtagactta ggggaaatac at
//
LOCUS       DAELLV010000026        27258 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.026, whole genome shotgun sequence.
ACCESSION   DAELLV010000026 DAELLV010000000
VERSION     DAELLV010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27258
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.026"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            169..702
                     /gene="pyrR"
                     /locus_tag="KPD58_000964"
     CDS             169..702
                     /gene="pyrR"
                     /locus_tag="KPD58_000964"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HBG0264747.1"
                     /translation="MVEKAQLMDEKAIARAITRISHEIIERNKGVENLVLVGIKTRGV
                     PIANRISKKIEQIEGTKVDTGDIDITLYRDDLEKIHVEPVVKGTYLDFNVNDKTVVLV
                     DDVLYTGRTVRASLDAIIDIGRPKSIQLAVLVDRGHRELPIRADYVGKNVPTSRHEII
                     SVSLLEIDGEDSVTIKE"
     gene            986..2197
                     /locus_tag="KPD58_000965"
     CDS             986..2197
                     /locus_tag="KPD58_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBG0264748.1"
                     /translation="MKKTIMSLQHLLAMFGATVLVPILTGFNPSVAIFCAGVGTLIFH
                     FCTEGKVPAFLGSSFAFIPVILAAKEAYGGDLAYAQGGIIVAGLIYIIMSIIVKIVGV
                     NKIKLYFPAQVTGAMIVVIGLNLLPTAFSMASANFVIASITLAIAILTNKFGRGFIKQ
                     LGILIAVFSGYIICLILRLVDITTITEASLFAIPNFTVPKFSLGAIVIISPVVLAVFM
                     EHIGDMTTNGAVVGKNFIENPGLNRTLLGDGFATIVAGCLGGPANTTYGENTAVLAIT
                     KNYDPSILRRTAIFAILLACVGKFGGFLQSIPGSVMGGISIMLFSMITYVGLKTIRDS
                     SCVESKINILIIAVILLIGLGTTYLSNKGISIGIPITSTVKITGLSLAAIVGIVLNRI
                     LNNQDFKVEEE"
     gene            2496..4148
                     /gene="abc-f"
                     /locus_tag="KPD58_000966"
     CDS             2496..4148
                     /gene="abc-f"
                     /locus_tag="KPD58_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein"
                     /protein_id="HBG0264749.1"
                     /translation="MLLVKVENLKKYYADKLILDIDKLEILENDKIGLVGSNGQGKTT
                     LLKAILGEIEIDEGYTYLTESYSYISQSENNIETCSHSKEKSLLNAPDKFEEHLSGGE
                     KVKLKIADALSNKKNIIIADEPTSNLDKKSIGVLEDMFKRHEGALLLISHDRRFLDEL
                     CTTILELEDGKLKAYKGNYTDYLMQKDEEVKRADFEYQEYVKEKKRLEKALLYKKALS
                     DGIRKTPKRMGNSEARLHKMGGQTNKKKLDSNVKAIKSRIDKLEVKNKPKVSKEMNIK
                     IQDGMEIISKNLVEVKDMTLKLENKLLLDNVSFKIKRGKKIALLGDNGCGKSTLIKEI
                     LADKNDNIKINNKVKVGYFDQNQSLLDEEKSVLYNTKVNSSFDESFIRINLSLFGFKG
                     DDVYKKVKVLSGGEKVKIALCKIILEDNNFLVFDEPTNYLDIKSMEALEKALINTDKT
                     MLIVSHDRVFVSHICNYIIEIKDAKIREFDCNYDEYTISRNKKTPSRENQIKKENLLV
                     LENRLTTVISMLSIEKDNLKKELYESEYNELLKQINKLKNSF"
     gene            complement(4363..6138)
                     /locus_tag="KPD58_000967"
     CDS             complement(4363..6138)
                     /locus_tag="KPD58_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HBG0264750.1"
                     /translation="MALDGLVIHSIVDELSSKLTGGKIDKIHQPEDDEVIFNIRNNKE
                     NFRLVLSASASNPRVYLTSNYQKENPLKAPMFCMLLRKYIQGGNIVEISQIGFERIIK
                     ISVESLDELKEKTVKNIMIEIMGRHSNIIITHGEENKIIDSIKRVPFSISRVRQVLPG
                     HDYSLPPEQNKLNPLDDISKDLFIKNLEELEGPIFKSIYSKFLGISPIIAKEICYRAG
                     VNQNAIIKDISDEQFDSLHKVFCNLFNDINSNKYSPCIIIDKKVDKVVDFSCINLTLF
                     SDLSYINKDSMSRILEDFYRTKDIKDRINQRSSDLKKSISVKLDRLYNKLKKQEEELS
                     ESENADIYKIKGELITSYIYMVEKGMESIEVANFYDENCNDVTIELNKNLTPSENAQK
                     YFKKYNKMKHAKVEISHQISLNKEEIDYLENIILSIENCENLAELQDIKEELAKVGYI
                     KTQKKNSKKDTIPSTKPHEFVSSDGFKILVGKNNKQNDYLTLRLADNDDLWMHTKNIP
                     GSHVIIKCAGKEVPDNTVFEGAMLAAFFSKSKLSSQVPVDYTKRKNVKKPSGSKPGMV
                     IYETNSTIYVTPEEETVAKLKVKSE"
     gene            6434..7468
                     /gene="ltaE"
                     /locus_tag="KPD58_000968"
     CDS             6434..7468
                     /gene="ltaE"
                     /locus_tag="KPD58_000968"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HBG0264751.1"
                     /translation="MRFIDLRSDTVTMPTDEMREAMAKAPVGDSIFRDDPTVNKLEEL
                     AAAKVGKEDAIFLPSGTFGNQLALFTHCLRGQEIIIGKGYHIVTHEVGAPAVIAGVQL
                     RTIDEDESGALNPELVEKAIRRDDIHEPQTGLICVENAYSGGTVVSLDNLREIKKIAE
                     KHNLPVHLDGARLFNAALTLGVEAKEIAKCCDSVMFCVSKGLAAPMGSILAGNKEFIA
                     KARRKQKVMGGGMRQVGIVAAAGIIAIEKMTLRLNEDHENAKYLACELNKIDGIEVNN
                     VNPDISMVFFKMSEDIIKEDVLIKEFFERNIKINKMENGEYRFVTHVDITKEDLDYVL
                     KTLKELIGVC"
     gene            7755..8585
                     /locus_tag="KPD58_000969"
     CDS             7755..8585
                     /locus_tag="KPD58_000969"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBG0264752.1"
                     /translation="MKFWKLHGIGNDFIAIDGRFDQINPSDYTALAKKVCHRRFSVGA
                     DGLLVVKDSKVADVEMVYYNSDGSRAAMCGNGLRCFCKFVYDNCIVKKESFTVDTLDG
                     IKDIKVNLTNREINSIRVNMGKGSFIAKDVPVLTNKERFVQESIKVLDRELKVTSMLM
                     GVPHTVVIVDELDVDDVCKYGKEIENNKIFPEKTNVNFVKVEDKYNIHVYTWERGCGY
                     TLGCGTGMTASAIVCNLLNMVESSVNVTSQGGTVKIDVGDFSYMTGPAVKICEGILEV
                     "
     gene            8589..9470
                     /locus_tag="KPD58_000970"
     CDS             8589..9470
                     /locus_tag="KPD58_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBG0264753.1"
                     /translation="MAISMTGFGRGEYKDDNYYFLVECKTINHKYSDINIRLPRKISF
                     LEDKVRNLVKNYVKRGRVDLYIKFDLLGKEDVNLNFDEGLASQYIDILKEIKNKFDII
                     DDISVMNVAKFPDIVKIEEKEEDEDLLWSMLNQAVEDALIKLREMRSEEGKKLAEDIA
                     MRCDLLKNHIEEIEKYSSSVVEDYREKLNLRISELLDDPSIIDENRLAQEVAIYADKS
                     SITEEIVRFKSHIGQLKNTIFKDDSIGRKIDFLIQEMNRETNTIGSKSSDINITNLVV
                     EVKSELEKIREQIQNIE"
     gene            9500..10117
                     /gene="gmk"
                     /locus_tag="KPD58_000971"
     CDS             9500..10117
                     /gene="gmk"
                     /locus_tag="KPD58_000971"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBG0264754.1"
                     /translation="MFVKKGLLLVVSGPSGAGKGTICKELLKENDTIKLSVSATTRKP
                     RTGEVDGVNYFFISKEKFEEMIEKGEFLEYAQIYDNFYGTPKAAIMECLEKGQDVLLE
                     IEMQGAKQIKEVCPEGVFIFVLPPSLKELKNRIVGRGTETEAEIEKRFSCAYEEIKMI
                     KDYDYFIFNEDVKTSAKEIEGIISSEKNKVSRYKNIIIEKFKEEL"
     gene            10120..10386
                     /locus_tag="KPD58_000972"
     CDS             10120..10386
                     /locus_tag="KPD58_000972"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBG0264755.1"
                     /translation="MLKPSINEVLEKIDNRYYLVGTVSKRARKLIDGEEPYVSNKTKE
                     KPVCVATKEVASGKITYRLLTEEEIEIEEARHHAEQHQQISEEE"
     gene            10391..11590
                     /gene="coaBC"
                     /locus_tag="KPD58_000973"
     CDS             10391..11590
                     /gene="coaBC"
                     /locus_tag="KPD58_000973"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HBG0264756.1"
                     /translation="MLKDKTVVIGISGGIAAYKACDVVSKLKKLNANIHVIMTKSATE
                     FVKPLTFQSLSQNYVVEDMFEEPKTWEVEHISLAKKADLFLIVPASANVIGKIANGIA
                     DDMLTTTVMATKAQVLIAPAMNTNMYENPIVQKNIQTLKSFGYKFIEPESGRLACGDI
                     GTGKLANPDIIVEEVLTNLTKEQDLKGQSMIVTAGPTVESIDPVRYLTNRSSGKMGYS
                     IAREAIARGADVTLISGPTNLSIPQNLKKFVQIESAQDLYEAVVSNLSENKIVIQSAA
                     VADYKPKTYSDKKIKKKNDDLSIELSRNYDIAYEIGKIKEDKILIGFAAETNDLIEHA
                     KGKIKKKNLDFIVANDLTKEGAGFRADTNIVKIIDVEGNITEYPKMKKEEVANVILDK
                     VKALLEK"
     gene            11829..14354
                     /gene="priA"
                     /locus_tag="KPD58_000974"
     CDS             11829..14354
                     /gene="priA"
                     /locus_tag="KPD58_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBG0264757.1"
                     /translation="MNNILNLSRGFIMKKYAKVIVRSNTIYTDNLFTYQIPVFLSDVI
                     KIGHRILVPFGKGNKPTEAFVFQFTDSLDEKIKIKEIIDILDENPIFRKEDLELVYWM
                     KNRYLCTYIECINLIYPKGYKLNNYKVVLLGESLSGLNDIELKEKISTLSDKNREIVE
                     KVIDSKGKIKVDKLKYIPNLNSSLYTMNKNGIIKLCWEYKNHKNEKKVCYISLSLESD
                     KIDDYIEQNKINVGSKQKEILSFLKNNENVEINDLLDLLNASKQSINSLSKKKLITLE
                     FKDYYREPKSIYKSVLKSIKLNNEQQEAVDEIKSNMFVDDKKPYLIHGVTGSGKTEVY
                     MEIIEFALNQGLDSIFLVPEIALTPQTIDRLKSRFGDLVGVFHSKLSEGEKHDVYKAV
                     KAGKVRVLIGARSALFAPFNSLGLIIIDECHESSYKSEKNPKFNAIEVARFMALKNNI
                     TLILGSATPSIEEYYRAKSGEYKLINIKSRANDKPLPNIEVVDMKDELDKGNRSIFSL
                     KLQKEIRYAIEENNQVILFLNRRGYANFVSCRKCGYVFQCENCDISLTYHKKSNTGRC
                     HYCGYEKEIPKECPECKSTYVKPFGVGTQKIEEELKYIFPDIKTLRMDKDTTSKKGAL
                     DEILNKFKDKEADVLIGTQMLSKGLDFENVTLVGILSADMILNFPDFKSFETTFQLIT
                     QVSGRAGRADKEGKVVLQTYDTEHYAIKHAIEYDYEGFYEDEIKIRKAFGYSPFNNML
                     SVVVSGEDERLVIKNIKNMHASLIYLLEKRGINDLGFILGPNPCSISKINQNYRWQIL
                     FKDENIEINLLKGIIKYICITKRDLIFDKNINVSIDINPNSVL"
     gene            14372..14812
                     /gene="def"
                     /locus_tag="KPD58_000975"
     CDS             14372..14812
                     /gene="def"
                     /locus_tag="KPD58_000975"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBG0264758.1"
                     /translation="MALRQIVQIGEPVLRKKSKKVEKIDEKIIQLLDDMAETMYDADG
                     VGLAAPQVGILKRVVVIDIGEELIELINPEIIETSGEQIDEEGCLSVVGEAGNVRRPN
                     YVKVRALNRNGETIELEGEELLARAFCHEIDHLDGILFVDKIEK"
     gene            14827..15756
                     /locus_tag="KPD58_000976"
     CDS             14827..15756
                     /locus_tag="KPD58_000976"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBG0264759.1"
                     /translation="MKIVFMGTPDIAVPCLQKIIDEKYEILGVVTQPDKPKGRGKKLG
                     MSPVKELAIENNIPVYQPVKARDKEFIDKIKSLNPDVIVVVAFGQILPKEILEIPKLG
                     CINVHVSLLPKYRGAAPINWVIINGEEKTGVTTMYMDEGLDTGDMILKTEVNLDENIT
                     AGELHDKMMNIGAETLKETLRLIEEGNAPREVQNHEEFSYAPIMNKSLGNIDFSKSAR
                     EIHNLVRGVNPWPSAYTTYNDVIMKVWKTKVLDEKSTKDVGTIIDVSKDGIKVSTIDN
                     VLLIEEIQMPNKKRMLVGEYIKGNTIETGLVLG"
     gene            15766..16518
                     /locus_tag="KPD58_000977"
     CDS             15766..16518
                     /locus_tag="KPD58_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HBG0264760.1"
                     /translation="MTDIKKYLTSVGILILILGILIGVVNFFIISLTQIFSLMINIII
                     ISLILIILCSTAVTYRVIKGKYVNSNIMKINFNIVSGLFPIISFIASSFGMSKSDIRR
                     IYIKLNNEYIYSNKYNFNPEDIIILIPHCIQENSCKLKVTNDIDNCKECGRCNIGELI
                     KLKEKTNVKIFVATGGTLARKIIMDNKPKAVVAVACERDLTSGIQDIKKIPVLGVFNK
                     RPNGPCVNTNVDIMDIEKAIGFLTGKNILVCN"
     gene            16587..17288
                     /locus_tag="KPD58_000978"
     CDS             16587..17288
                     /locus_tag="KPD58_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HBG0264761.1"
                     /translation="MYPVYGGFWGFDPTMVVLIPAILLTIYAQFKVSSTTNKYLRVNT
                     RRGYTGEQTARRVLDSNGLYDVKIEMVRGHLSDHYDPRRKAVRLSEDVYYGTSITSVA
                     VAAHECGHAIQHAKGYAPLQIRSSLVPVVNFASSISWFLIFLGFIMAGPFLKIGILLF
                     SASVLFQIITLPVEFNASSRAIVQLGNLGIIDESESRQSRRVLSAAALTYVAAALVSI
                     LQLLRLLLIAQRRND"
     gene            17350..18675
                     /gene="rsmB"
                     /locus_tag="KPD58_000979"
     CDS             17350..18675
                     /gene="rsmB"
                     /locus_tag="KPD58_000979"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBG0264762.1"
                     /translation="MDAREVGFKVLCDIEKNDNYSNIAINKHFKNLEISDMDRGLATE
                     LIYGVIENKYYLDYIINKLSKIKVKKMSTYVKIFLRMGTYQILFLDSISDYAAVNETV
                     KLSKKYDKKSSGFINAILRNEIRAKETIMDITEEDSVKYLSIKYSYNSWIIKNWIDKF
                     GQEFAEDLLEANNEKPSIYIRTNTLKISREELIEKLSEMGIVCLKVPMVEEAIKVEKM
                     KNIEKNELFKAGLFTIQDISSMIVGKVINPKEGSFVLDVCSAPGGKSTHLATLMNNTG
                     QVVARDIFEHKLKLINSTVNRLGLKNVFVERFDASEIDENSISKFDYVLADVPCSGFG
                     IIRRKPEIKYKKEEELKDITSIQKKILENASKYVKVGGTLVYSTCTIQDVENINIVTS
                     FVEENHNFEFVPIDTVNVDLDNQDKGYLKIYPNIHGIDGFFIAKLKRIR"
     gene            18683..19714
                     /gene="rlmN"
                     /locus_tag="KPD58_000980"
     CDS             18683..19714
                     /gene="rlmN"
                     /locus_tag="KPD58_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBG0264763.1"
                     /translation="MEKKKIVLKNFTEDELKEFMKTIDEKPFRGSQIFSWIYKGAKTF
                     DDMNNIPKSLRNKLEEVSCIGHIDIELKLESKVDNTKKYLFLLDDGNIIETVMMDYDS
                     RVTVCVSNQVGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGS
                     GEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLH
                     SPYDEERRKIMPVANAYSIEEILNACRYYIKKTNRRVTFEYSLIKGVNDSEKEAKALA
                     KLLKGMLCHVNLIPINKVEEREYEKPDKAFIYKFRDSLEKNNIPATVRMSMGSDISGA
                     CGQLRRKYK"
     gene            19734..20486
                     /locus_tag="KPD58_000981"
     CDS             19734..20486
                     /locus_tag="KPD58_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HBG0264764.1"
                     /translation="MVYSCASHIGKIRKNNEDYCEGEVIDTEHGPIGIFAIADGMGGH
                     KKGEVASKLAVENIIDFLKENLLQHDNVKIDYIDDILKQAYNNVNSIVHKKSMEDIEF
                     EGMGTTLVTAIVYNNVLYVANVGDSRCYLLTDEKFDKITIDHSVVEELMMAHVITEEE
                     ARRHPQRNRITRAIGTDDMVVVDIFKKEIKKSDIILLATDGLTGFIDDEDIRDLILDY
                     EYERTSNISEELISMANDVSGKDNVSVIVIKV"
     gene            20503..22518
                     /gene="pknB"
                     /locus_tag="KPD58_000982"
     CDS             20503..22518
                     /gene="pknB"
                     /locus_tag="KPD58_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HBG0264765.1"
                     /translation="MGDTILGNRYEIIRKIGDGGMAFVYEAKDRLLNRTVALKVLRPE
                     FVDDDEFLTKFKREAEAVASLSHPNIVNVYDVGEDGKVHYIVMEFVDGKNLKEIIQDE
                     GILDEYTALDITKQIAMALSAAHKKGIIHRDIKPHNILISNEGRVVKVADFGIAKAVS
                     NSTMTNIGSIIGSVHYFSPEQAKGKFVTNNADLYSLGIVLYEMLIGKVPFRGDSPISI
                     ALQHINDDIDFTSEEKVRIPQSVRTTIKKLTEKSSADRYQTAEELIEDIEYIEKNIDL
                     DFIKEYDDFATKKIDEKEINKVVNPTLAKPAPEKVVKPVEVADLDDDEDYYDDFYEED
                     DDEDEEEEEIMRAKKNQRPKSTPSKRTKKKKKKQESPKSRRRLKVIAAVLILILCAQV
                     FLAYKFLFAGGFGNKSLTVPNLVNMTLEEAQSAVEKEGLYLSVKSEEYNSEVDENCII
                     SQTPEGGSTNVKKGDTINVVVSKGSSQASVPNVVGLTLSNAKQLIEENNLKVGTVKYE
                     YSSIYKEGTVLSQSPGAGSSRAQEGDEVNLYVSKGSEKSNTQTPTVPDKKPTTPENNT
                     PTEPGSNSGNSGGNSGGSNSGNSGGNSGNNGSNSGGSNSGNSGGNSGGSNSGDNGGSS
                     GGSNSGDNGGSSGGSNSGDNGDSSGGSNSGDNGGTSSGTGANIGKTE"
     gene            22626..23363
                     /locus_tag="KPD58_000983"
     CDS             22626..23363
                     /locus_tag="KPD58_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBG0264766.1"
                     /translation="MKKKTVLITGGARGIGKAMSKAFAKEGYNVLVNFNKSENEAKEL
                     YTILNEKNFSVKLFKADISNREDVEDMVDYCIKEFGGLDVLVNNAGVSQDKLFTDITD
                     EDWDNMMNINLKGSFYCSQVALKYMISEKKGNIINISSIWGISGASCEVHYSITKAGI
                     IGMTKALAKEVGPSNIRVNSIAPGVINTDMLSGYNEEDIDALVEETPLMRLGTPEDIA
                     NCAIFLASDKSNFITGQVISPNGGFVI"
     gene            23461..24345
                     /gene="rsgA"
                     /locus_tag="KPD58_000984"
     CDS             23461..24345
                     /gene="rsgA"
                     /locus_tag="KPD58_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBG0264767.1"
                     /translation="MLEGKIIKGISGFYYVDTYNGIYECKARGIFRKQKITPLVGDRV
                     KISIVDENEKKGILEEIDSRDTELIRPPIANVDKALIVFAIKNPKPNLSLLDRFIVLA
                     EKENLETVIILTKADLDDNDTLETVKNIYELSGYKVIPVSNITKLNIDKVKEELKENV
                     VVFAGPSGVGKSSLLNEIDENFKLQTGVVSDKIKRGKHTTRHAELLKLEFGGMVADTP
                     GFSSLALEDIEEVELKDYFIEFDKFNDCKFGSKCIHENEPNCAIKEAVTNGEISKERY
                     DSYIQLLHEIRQNNSRRY"
     gene            24350..25021
                     /locus_tag="KPD58_000985"
     CDS             24350..25021
                     /locus_tag="KPD58_000985"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBG0264768.1"
                     /translation="MIKLAPSILSADFAKLLEDVRKVESAGCEYLHIDVMDGHFVPNI
                     TLGPLVVKSLKKENINMVFDAHLMIENPDQYIEEFVKAGCDIITVHQEACVHLHRTIQ
                     NIKSHGIKAGVVLNPATPVDTIKHVLPDLDMVLLMSVNPGFGGQSFIPCVLDKIKELK
                     AIIDSQGLNIDIEVDGGISPKNVAEVVQAGANVIVAGSAIFGSDDIQETVNLFRKNAS
                     LEELV"
     gene            25030..25671
                     /locus_tag="KPD58_000986"
     CDS             25030..25671
                     /locus_tag="KPD58_000986"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HBG0264769.1"
                     /translation="MKICIVLNGEIEDYEVTRDIIIKECYDCIICADGGANHTYKMEI
                     MPDYILGDLDSVEEEKINFYKNKGVKFEKFPSKKDETDTELCLFLAKTLKANHIDFFG
                     ALGGRIDHTLANIKLLYYLKEDGIYSRILSDKEEMYIVENEEISLYGNPGDTISVIAI
                     NGDAKGVTLTGLEYPLDNYYMKYSVPIGISNVMLSNSCKIKVEQGCVLVVRNL"
     gene            25802..26431
                     /locus_tag="KPD58_000987"
     CDS             25802..26431
                     /locus_tag="KPD58_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBG0264770.1"
                     /translation="MVDSIIFDLDGTLWDSTEEVCKVWQDVLSEHKEIELSVTKDLFR
                     SLMGLSFEEIANRLFPNLSEEERMNLLNECSLKECDYLSENGAKLFDDIEDTLKLLSK
                     KYKLFIVSNCQVGYIESFLKAHKLEQYFIDFECPGNTGLHKGENNKLIIERNKLINPI
                     YVGDTQGDANSAKFVGIPFIYAKYGFGNVDVYDYFIDSFKDLLEHDILK"
     gene            26486..27142
                     /locus_tag="KPD58_000988"
     CDS             26486..27142
                     /locus_tag="KPD58_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG0264771.1"
                     /translation="MELQDTIFKRQSVRKFKNQDVSDEDILKMIKAAGAAPSGKNIQN
                     WHFVVIKRRDLMEKIADVITKKQQEILVEMDKVSVDKANRFRKFVKNFTLFYLKAPVL
                     VLVFTKVYNPSGYYELELIDAPKETIDKLFIRNPGMQSLGAAIENFTLSAIELGYGSC
                     WLTSQNYAADEIEAVLEAETGFEKGEYFLGAMLALGVPEDNLKSPSKKPVEEICTFIK
                     "
BASE COUNT        10849 a         2766 c         5083 g         8560 t
ORIGIN      
        1 ttgctaaatt gcactgtata ttttttatat aagagcttct tgtcttgtga tgagaagttt
       61 ttttgttgtt taaattgaaa ttttaaataa aaataaattt ataaaaaatt aaaagaaagg
      121 agtagatttg tcaaatagat atattatatc tttgacaata attatgatat ggtagaaaaa
      181 gctcaattaa tggatgaaaa ggcaatagca agagcaatta ctagaataag tcatgaaata
      241 attgaaagaa ataaaggtgt agaaaatcta gttttagttg gaataaagac tagaggtgtg
      301 ccaattgcaa atagaatttc taagaaaata gaacaaatag aaggaacaaa agtagataca
      361 ggagatattg atataacttt gtatagagat gatttggaaa aaattcatgt tgaacctgta
      421 gttaaaggaa cttatttaga ttttaatgta aatgataaga cagtagtttt agttgatgat
      481 gttttataca ctggaagaac tgtgagagct tcactagatg ctattataga tataggaaga
      541 cctaaatcaa tacaattagc agtgctagtg gacagagggc atagagagtt gcctataaga
      601 gcagattatg taggaaaaaa tgtacctact tctagacatg aaattatatc tgtcagttta
      661 ttagaaattg atggagaaga ttcagttaca atcaaagaat aattattttt aagtattata
      721 taaaagctta gcattgaata gtcagggagg aggataaagc ttacatagat tgcacaaaat
      781 aaatattata attttatgga tgtgagtctt aatgtagttt ttattctcct ttatttgagt
      841 ttttgatgtc gtaaaatgta aaataatgat aaattatata ataagtatat agaggttaaa
      901 aaatagaatc tagggaatga taattaatta aaagatatta attaaacaga ggatttattt
      961 atatataata attttggagg tttttatgaa aaaaacgata atgtctttac aacatctatt
     1021 agccatgttt ggagcgacag tattagttcc cattttgaca ggttttaatc cgtcagttgc
     1081 aattttctgt gctggtgttg gtacattgat atttcatttt tgtacagaag gaaaagtacc
     1141 tgccttttta ggttcaagtt ttgcgtttat accagttata ctagcagcta aagaggcata
     1201 tggtggagat ttagcatatg ctcaaggtgg tataatagta gcaggattaa tttatattat
     1261 aatgtcgatt atagtaaaaa tagttggtgt aaataaaatt aaattatatt ttcctgcaca
     1321 agttacagga gctatgatag tagttatagg attaaattta ttgccaacag cttttagtat
     1381 ggcttcagcc aattttgtaa tagcatctat aactttagca atagcaattt taaccaataa
     1441 gtttggtaga ggatttataa aacaattagg aatattaata gcagtttttt ctgggtatat
     1501 aatctgtttg atattaagat tagtagatat aactactatt acagaagcaa gtttatttgc
     1561 aatacctaac tttacagttc ctaagttcag tttaggagca attgtgatta tatctccagt
     1621 tgtattagct gtatttatgg aacatatagg agatatgact actaatggtg ctgtagtagg
     1681 taaaaacttt atagaaaatc ccggattaaa tagaacatta cttggggatg gatttgcgac
     1741 tatagttgca gggtgtttag gaggaccagc aaatacaact tatggagaaa atacagcagt
     1801 acttgctata actaaaaact atgacccttc aatacttaga cgtacagcaa tatttgctat
     1861 attgcttgca tgtgtaggaa agtttggtgg atttcttcaa agtataccag gttcagttat
     1921 gggtggaatt agtataatgc tattttcgat gataacttat gtaggattaa aaactataag
     1981 ggacagttct tgtgtagaaa gtaaaataaa tatattaatc atagcagtta tattattaat
     2041 aggtcttgga accacatatt tatctaataa aggtatttct attggaatcc ctataacaag
     2101 tacagtaaag ataactggtc ttagtttagc tgcaattgtt ggaattgtat taaacagaat
     2161 tttaaataat caggacttta aagttgaaga agaataaatt taaagaataa gttgtatatt
     2221 gaataactaa aattagttaa ttttctatag acatttgtaa ttaatgttga tataatgatt
     2281 aaaaataaat gaaagaaagg gcaaggtaac taggacaatg agaatataat ctatttttta
     2341 taatttaaag tattgtaatt tttgaaaagc ctattttggt ggctttattt gcaatgctta
     2401 aattaaggag agtagattat agtctatacg tttatttgtt atgcctattt atagaatctc
     2461 tccttatatt taagtgtgtt aaggagggat tttttatgtt gttagtaaaa gtagagaatt
     2521 taaagaaata ttatgcagat aaattaattt tggatataga taaacttgaa atattagaaa
     2581 atgacaaaat aggtctagtt ggttcaaatg gtcaaggaaa aacaacatta ttaaaagcaa
     2641 tattgggaga aatagaaatt gatgaaggat atacttacct tacagaaagc tattcttata
     2701 taagtcagag tgaaaataat attgaaacat gtagtcatag taaggagaag agtcttttaa
     2761 atgctccaga taaatttgaa gaacacttat caggagggga aaaggttaag ttaaaaatag
     2821 cagatgcgct gagtaataag aaaaatatca taatagctga tgagccaact tcaaacttag
     2881 ataaaaaaag cattggggtt ttggaagata tgtttaaaag gcatgaggga gcattattat
     2941 taatatctca tgatagacgt tttctggatg agttatgtac aactatatta gaactagaag
     3001 atggaaaact aaaagcttac aaaggcaatt acactgatta tttaatgcaa aaagatgaag
     3061 aagtaaaaag agctgatttt gagtatcaag aatatgttaa agagaaaaaa aggcttgaaa
     3121 aagctctttt atataaaaaa gctttaagtg atggcataag aaaaacacca aaaagaatgg
     3181 gtaattcaga agctaggttg cacaaaatgg gtggtcagac taataagaaa aagttggact
     3241 caaatgtgaa ggctataaaa agcagaattg ataaacttga ggtaaagaat aaacctaaag
     3301 tttctaaaga aatgaacatt aagattcaag atggtatgga gataattagt aaaaatctag
     3361 tagaggtaaa agatatgact ctaaagttag aaaataagct cctgttagat aatgtttcct
     3421 ttaagataaa aagaggtaaa aagatagcat tattaggtga taatgggtgt ggaaaaagta
     3481 ctctgataaa agaaatattg gctgataaaa atgacaatat aaaaataaat aacaaggtaa
     3541 aagtaggtta ttttgaccaa aatcaaagtt tattagatga ggaaaagagt gttctataca
     3601 atactaaagt taatagttca tttgatgaat cttttataag gataaactta agcctatttg
     3661 ggtttaaagg tgatgatgtc tacaaaaaag taaaagtact tagtggtgga gaaaaggtaa
     3721 aaatagcact atgtaaaata atattggaag ataataattt tttagtattt gatgaaccaa
     3781 caaattactt agatataaaa tctatggaag cactggaaaa ggcactgata aatactgata
     3841 aaactatgct tatagtgtct catgatagag tatttgtatc acatatttgt aactatatta
     3901 tagagataaa agatgctaaa ataagggagt ttgattgtaa ttatgacgaa tatacaatta
     3961 gcagaaataa aaaaactcct agtagagaaa accaaatcaa aaaagaaaat cttctagtat
     4021 tagaaaatag acttacaact gttatatcta tgttatctat agaaaaagat aatctaaaaa
     4081 aagaattgta tgaatcagaa tataatgaat tactaaaaca aatcaataaa ttaaaaaata
     4141 gcttttagtg ttaacttttt aattacatct atataagaag actgaaaata tttgttggga
     4201 atataagtat agttattaaa acagaaaagt attttgttag aaaattaaaa gtaagctaat
     4261 tagaaagtgg ccttattatt gtactttata agaaggccac tatttttatt tagaatgata
     4321 atgtgtagtt gtctaacatt tattttgtat ttagtgcaga atttattcag atttaacctt
     4381 aagcttggct actgtttctt cttcaggagt aacataaata gtactgttag tttcataaat
     4441 aaccatacct ggtttagaac cactaggttt tttcacattt tttctcttag tatagtctac
     4501 aggcacctga gaagaaagtt ttgacttact aaagaatgct gctagcatag ctccttcaaa
     4561 tacagtatta tcagggactt ccttaccagc acactttatt attacatgag aaccaggaat
     4621 attctttgta tgcatccata agtcatcatt atcagcaagt cttagtgtaa gatagtcatt
     4681 ttgcttattg tttttaccaa ccaatatttt aaatccatca gaagaaacaa attcatgagg
     4741 ttttgtagat gggatagtat cctttttact gtttttcttt tgagtcttga tgtatccaac
     4801 cttagcaagt tcttctttta tatcttgtaa ttcagctaaa ttttcacagt tttcaatact
     4861 taaaattatg ttttctaagt aatctatttc ttccttattt aaggaaattt gatgagatat
     4921 ctcgacttta gcatgtttca ttttgttata ctttttaaag tatttttgag cattttcaga
     4981 tggtgtcaag tttttattta attctattgt tacatcatta caattttcat catagaagtt
     5041 agcaacttca atactttcca tacctttttc taccatataa atatatgaag taatcaattc
     5101 tccttttatc ttataaatgt ctgcattttc ggattctgat aattcttctt cttgtttttt
     5161 aagtttatta taaagtctat caagcttaac agatatactc ttctttaaat cagatgaacg
     5221 ttgattaatt ctatctttaa tatccttagt tctatagaaa tcttccagaa tacgagacat
     5281 actatcctta tttatatatg ataaatcgct aaataatgta aggtttatac aactgaaatc
     5341 aactacctta tccacctttt tatctataat aatgcaagga gagtatttgt tagaatttat
     5401 atcattgaat aaattacaaa atactttgtg taaagaatca aattgttcat cagatatatc
     5461 ctttatgata gcattttgat ttacacctgc tctgtagcaa atttctttag ctataatagg
     5521 gcttatacct agaaatttag aatatataga cttaaagata ggtccttcca attcctctag
     5581 gtttttaata aacaaatcct tagatatatc atcaagaggg ttgagtttgt tttgttctgg
     5641 tggcaatgaa taatcatgac caggtaaaac ttgacgaact ctacttatgc taaaagggac
     5701 tctttttata gaatctataa ttttattttc ttcaccatga gttattatta tgttgctatg
     5761 tcttcccata atttcaatca taatattttt aactgtcttt tcttttaatt catctaaaga
     5821 ttcaacactt atttttatta ttctttcaaa acctatttga gaaatttcta ctatatttcc
     5881 accttgtatg tattttctaa gtaacataca aaacataggc gcttttagag ggttttcttt
     5941 ttgataatta cttgtaagat aaactctagg atttgaggca cttgcactta aaaccaatct
     6001 aaaattttct ttattatttc ttatattaaa aattacctca tcatcttcag gttgatgtat
     6061 cttatctatt tttccacctg taagttttga tgataattca tcgactattg aatgtataac
     6121 taatccatct aatgccattt atagcacccc tttctttgac caaaatgagt cagtaaatta
     6181 attatacaca taatatacgc tttgtgacta gggggaattt aaacaataga gattaatatt
     6241 taaattgtat aattatttat atttattaga gaggaaactt tatttttaaa attaataatt
     6301 taaagtagat taaatataaa gtaaatatag tataattatt gtgtagagta taagctatga
     6361 aagtatatga actttacaga aaaaatcaat tttaaggttc tatagtatat aaatatgaaa
     6421 gaaggttgaa aaaatgagat ttatcgattt aagaagtgat actgttacta tgcctactga
     6481 cgaaatgaga gaagctatgg caaaggctcc tgttggagat tcaatcttta gagatgaccc
     6541 tactgtaaat aaacttgaag aattagcagc agctaaagtt ggaaaagaag atgctatttt
     6601 ccttccaagt ggaacttttg ggaatcaatt agctctattt actcattgcc taagagggca
     6661 agaaataatt attggtaaag gttatcacat tgtgactcat gaggttgggg cacctgctgt
     6721 aatagctgga gttcagctta gaactataga tgaagacgaa agtggagctc taaatcctga
     6781 acttgttgaa aaggcaatta gaagagatga catacatgaa ccacagacag ggcttatatg
     6841 tgtagaaaat gcatattcag gtggaacagt tgtgagtctt gataatttaa gagaaattaa
     6901 aaaaatagct gagaaacata acttaccagt acatttagat ggagcaagat tatttaacgc
     6961 agcattaact ttgggtgtag aagctaaaga aatagcaaag tgttgtgata gtgttatgtt
     7021 ctgtgtgtca aaaggtttag ctgcaccaat gggttctata cttgctggaa ataaagaatt
     7081 tatagcaaaa gctagaagaa agcaaaaagt tatgggtgga ggaatgagac aagtaggtat
     7141 agttgcagca gctggtataa ttgccattga aaaaatgact ttaagattga atgaagacca
     7201 tgaaaatgca aaatatttag cttgtgaatt aaataaaatt gatggcattg aagtaaacaa
     7261 tgttaatcca gatataagta tggtattttt taagatgagt gaagatatta taaaagagga
     7321 tgttttaata aaagaatttt ttgaaagaaa tataaaaatt aataaaatgg aaaatggtga
     7381 atatagattt gtaactcatg tagatattac taaggaagat ttagactatg ttttaaaaac
     7441 acttaaagaa ttaataggtg tatgttaata gatatctact agaaggatta tagctagtta
     7501 ccagatgttt aattaaaaaa ttcatagaca gtttaaataa aacatacata aaattcatat
     7561 agaaatatta atatataaac atagaaagaa ctagcttaaa aacataatat aattacccta
     7621 attgtaatgg caactctccc tcacatcctc aaattatagt tgtcattaca aaacttttcc
     7681 cataaattat aagtttatgg gaattttttt atgttataat agtttaagtg ttaatttacg
     7741 aaggagcgat acttatgaaa ttttggaaac tacatggaat aggaaatgat tttatagcaa
     7801 tagatggaag attcgaccag ataaatccat ctgattatac tgctttagca aaaaaagtat
     7861 gccatagaag attttctgta ggagcggatg gattgttagt tgtgaaagat tcaaaagttg
     7921 cagatgtaga aatggtatat tataattctg atggttcaag agcagctatg tgtggaaatg
     7981 gccttagatg tttttgtaaa tttgtttatg ataattgtat agttaaaaaa gaaagtttta
     8041 cagtagatac attagatggt ataaaagata taaaagtaaa tcttactaat agagaaataa
     8101 actctataag agtcaatatg ggaaaaggaa gttttatagc taaggacgtt cctgtactta
     8161 caaataaaga acgttttgtg caagaaagta taaaggtttt agatagggag cttaaagtta
     8221 cttctatgtt gatgggggtt cctcatacag tagttatagt agatgaatta gatgtagatg
     8281 atgtttgtaa atatggaaaa gagattgaaa ataataaaat atttcctgaa aaaacgaatg
     8341 taaattttgt aaaggtagaa gataagtata atatacatgt ttatacttgg gaaagaggtt
     8401 gtggatatac tttaggttgt ggtactggta tgactgcatc agcaatagtg tgcaatttac
     8461 ttaatatggt agaaagtagt gtaaatgtaa cgtctcaagg tggaacagtt aaaatagatg
     8521 taggagattt ctcttatatg acaggtccag cagtaaaaat atgtgaaggt attttggagg
     8581 tttaattaat ggctataagt atgactggtt ttggaagagg agaatacaag gatgataatt
     8641 attacttttt agtagagtgt aaaacgataa atcataagta ttcagatata aacatacgtc
     8701 ttccaagaaa aatttcattt ttagaggata aagttagaaa tttagtaaaa aattatgtaa
     8761 aaagaggaag agtagattta tacataaagt ttgacttatt aggcaaagaa gatgtaaact
     8821 taaattttga tgagggatta gcatctcagt atatagacat actaaaagaa ataaaaaata
     8881 aatttgatat tatagatgat attagcgtta tgaatgttgc aaagtttcct gatattgtga
     8941 aaatagaaga aaaggaagaa gatgaagatt tattgtggtc aatgttgaat caagcagttg
     9001 aagatgcatt aatcaaatta agagaaatga gaagtgaaga aggtaaaaaa ttagctgaag
     9061 atattgccat gagatgtgat ttattaaaaa atcatataga agagattgaa aaatattcaa
     9121 gtagtgttgt agaagattat agagaaaaat tgaatttgag aataagtgaa ttactggatg
     9181 acccaagtat aatagatgag aatagattag cacaagaagt tgcaatatat gcagataaaa
     9241 gcagtataac agaagaaata gttagattta aaagtcatat agggcaactt aaaaatacta
     9301 tatttaaaga cgactctata ggtagaaaaa tagatttctt aatacaagag atgaatagag
     9361 aaactaacac tataggttcg aagtcatctg atataaatat aactaattta gtagtagaag
     9421 taaaaagcga attggaaaaa ataagagaac aaatacaaaa tatagagtaa gaatacaata
     9481 ttagaagggg aatgcttaaa tgtttgtgaa aaaaggatta ttgctagttg tatcaggacc
     9541 atcaggtgca ggaaaaggta ctatttgcaa agaactactt aaagaaaatg atacaataaa
     9601 actttctgta tcagctacta caagaaaacc cagaactgga gaagttgatg gagtaaatta
     9661 tttcttcata agtaaggaaa agtttgaaga gatgatagaa aaaggtgagt ttttggagta
     9721 tgctcaaatt tatgataatt tttatggaac tccaaaagcg gcaataatgg aatgtttaga
     9781 aaaaggacaa gatgtattac tggagattga aatgcaagga gcaaaacaaa taaaagaagt
     9841 ttgcccagag ggagtattta tatttgtact accaccatca cttaaagaat taaaaaatag
     9901 aattgttgga agaggtacag aaacagaagc tgaaatagag aagagattta gctgtgcata
     9961 tgaagaaatt aaaatgatta aggattatga ttattttata tttaatgaag atgtaaaaac
    10021 atctgctaaa gaaatagaag gtataatatc atctgaaaag aataaagtta gcagatataa
    10081 aaatattata atagagaaat ttaaggagga attataatta tgttaaaacc atctatcaat
    10141 gaggtactag aaaaaataga caacagatac tatttagtag ggactgtttc aaagagagca
    10201 agaaaattaa ttgatggaga agaaccatat gtttcaaata aaactaagga aaagcctgta
    10261 tgtgtagcaa ctaaagaagt tgcaagtgga aaaataacat atagattatt gacagaagag
    10321 gaaatagaga tagaagaagc tagacatcat gctgagcaac accaacaaat ttcagaagag
    10381 gagtaaggtc atgctaaaag ataaaactgt tgtaattggc ataagtggcg gtatagctgc
    10441 atataaggct tgtgatgttg taagtaagtt aaaaaaacta aatgcaaata tccatgttat
    10501 aatgacaaaa tcagcaacag aatttgttaa accacttaca tttcaatcgc tgagtcaaaa
    10561 ttatgtagta gaagatatgt ttgaagaacc aaaaacttgg gaagttgaac atatatcatt
    10621 agcaaaaaaa gcagatttat tcttaatagt gccagcaagt gcaaatgtta taggcaagat
    10681 tgcaaatggt atagctgacg atatgttgac aactactgtt atggcaacaa aagcacaggt
    10741 tcttatagca ccagctatga atactaatat gtatgaaaat ccaattgttc aaaaaaatat
    10801 acaaacttta aagagctttg gatataaatt tattgaaccg gaaagtggaa ggcttgcatg
    10861 tggagatatt ggaacaggta aactagctaa tccagatata atagtagagg aagttttgac
    10921 taatcttact aaagagcaag atttaaaagg tcaaagtatg atagttacag ctggtccaac
    10981 tgtagagagt atagacccag taagatattt aactaatagg tcttctggta aaatgggata
    11041 ttctatagca agagaagcta tagctagagg tgctgatgta acattaatat caggtcctac
    11101 aaatctttct attccacaaa atcttaaaaa atttgttcaa atagagtcag cacaagattt
    11161 gtatgaagca gtagtatcaa atttaagtga aaataaaata gtaattcaaa gtgcagcagt
    11221 tgcagattat aaaccaaaaa cttattctga caaaaaaatt aaaaagaaga atgatgattt
    11281 atcaatagaa ctaagtagaa attatgatat tgcttatgaa ataggaaaaa taaaagaaga
    11341 taagatacta attggatttg cagctgaaac taatgacctt atagaacatg ccaaaggaaa
    11401 aataaaaaag aaaaatttag attttattgt ggcaaatgat ttaactaaag aaggtgctgg
    11461 ttttagagct gatactaata ttgtaaaaat aatagatgta gagggaaata ttactgagta
    11521 tcctaagatg aaaaaagaag aagtagccaa tgttatttta gataaagtta aagcgttgct
    11581 tgagaaataa gacctttttt aaatatgata tttgtgtatt ttatcacagc taaagtggct
    11641 gaaagctgca atttaatttt agcgagtaaa aactcgcaaa tgtcagttaa attgtagttt
    11701 tcagcattta ttttgtcata aagtataaaa tataattaat agttaattat taaaataaag
    11761 ttaatattaa taacttgaaa ttgcttataa attctgagat tttaatatct tgtaaagtat
    11821 attaaaatat gaacaatata ttaaatttaa gtagaggttt tataatgaaa aaatatgcaa
    11881 aggtaatagt tagaagtaat actatatata cagataattt atttacatat caaattcctg
    11941 tatttttatc tgatgtaata aaaattgggc acagaatatt agtaccattt ggaaaaggta
    12001 ataaaccaac agaagccttt gtatttcaat ttacagatag cttagatgaa aaaataaaaa
    12061 taaaagaaat tattgatata ttagatgaaa atccgatatt tagaaaagaa gatttagagt
    12121 tggtttattg gatgaaaaat agatatttgt gtacttatat agaatgtata aatttgatat
    12181 atccaaaggg atacaaatta aataattata aagtagtcct attaggtgaa agtttaagtg
    12241 gattaaatga tattgaattg aaggaaaaaa tatcaacttt aagtgataaa aatagagaaa
    12301 tagtagagaa agtgattgat agtaaaggta aaattaaggt tgataagtta aaatatatac
    12361 caaacttaaa tagttctttg tacacaatga ataagaatgg aattataaaa ttatgttggg
    12421 aatataaaaa tcacaaaaat gaaaagaagg tatgttatat atctttgagt ttagaaagtg
    12481 ataagattga cgattatata gagcaaaata aaataaatgt gggaagtaaa caaaaagaga
    12541 tattaagttt tcttaaaaat aatgaaaatg tagagataaa tgatttattg gatttattaa
    12601 atgcttcaaa acaaagtata aattctctaa gtaagaaaaa gctaataacc ctagaattta
    12661 aggattatta tagagagcca aaaagtattt acaaatccgt actaaaaagt ataaaattaa
    12721 acaatgagca acaagaagca gttgatgaga taaagtcgaa tatgtttgta gatgataaaa
    12781 aaccatactt aattcatgga gtaactggaa gtggaaaaac tgaagtttac atggaaataa
    12841 tagaatttgc tctaaatcaa ggcttagaca gtatattctt agtacctgag atagcattaa
    12901 ctcctcaaac aatagatagg ctaaagagta ggtttggaga tttggttgga gtgttccaca
    12961 gtaaactatc tgaaggagaa aaacatgatg tttacaaagc tgtcaaagca ggtaaagtaa
    13021 gagtgttaat tggagctagg tcagctttat ttgcaccttt taattcctta ggcttaataa
    13081 taattgatga atgtcatgag tcatcatata agtctgagaa gaatccaaaa tttaatgcta
    13141 tagaagttgc tagatttatg gcattaaaaa ataatataac tcttatttta ggttcagcaa
    13201 cgccctcaat tgaagagtat tatagggcaa aatctggaga atacaaactt ataaatataa
    13261 aaagtagagc aaatgataag cctcttccaa acattgaagt ggtagacatg aaagatgagt
    13321 tagataaagg aaatagaagt atatttagtt tgaaattaca aaaagaaata cgttatgcga
    13381 ttgaagaaaa taatcaagta atactatttt taaacagaag aggttatgca aattttgtat
    13441 cttgtagaaa gtgtggatat gtgtttcaat gtgaaaattg tgatatatct ttaacttatc
    13501 ataagaaaag caatactgga agatgccact attgtggata tgagaaagaa attccaaaag
    13561 agtgtcctga atgtaaaagt acttatgtta agccatttgg agttggaact caaaaaattg
    13621 aagaggaatt aaaatatatt tttccagata taaaaacttt gagaatggat aaagatacaa
    13681 cttcaaaaaa aggtgcttta gatgagatac taaataaatt taaagataaa gaagcagatg
    13741 tacttattgg aacacagatg ttaagtaaag ggctggattt tgagaatgta actttagtag
    13801 gtattttgtc tgcagacatg atattaaatt ttccagactt taaaagtttt gaaactacat
    13861 ttcaattgat aactcaagtc tctggaagag cagggagagc tgataaagag ggaaaagtag
    13921 tacttcagac ttatgacact gaacactatg ctattaagca tgctattgaa tatgattatg
    13981 agggttttta tgaagatgag ataaaaataa gaaaagcatt tggatattct ccttttaata
    14041 atatgttaag tgtagttgtt agtggtgaag atgaaagact agtgataaaa aatataaaaa
    14101 atatgcatgc atcattgatt tatttattag aaaaaagggg tataaatgat ttaggattta
    14161 tattaggtcc aaatccatgt tcaatatcaa aaataaatca aaattataga tggcaaatac
    14221 tctttaaaga tgaaaatatt gaaattaatc tcttaaaggg tataataaaa tatatatgta
    14281 taacaaaacg agatttgata tttgataaga atatcaatgt atctatagat attaatccaa
    14341 acagtgtatt ataaatttag gaggaattat aatggcttta agacaaatag ttcaaatagg
    14401 agaaccagta ttaagaaaaa aatcaaaaaa ggttgaaaaa attgatgaaa agataataca
    14461 attattagat gatatggcag aaactatgta tgatgcagat ggtgttggac tggctgcacc
    14521 tcaagttgga atattaaaaa gagtagtagt aatagatatt ggagaagagc ttatagaact
    14581 tataaatcct gaaataatag aaacatctgg ggagcaaata gatgaggaag gatgtttaag
    14641 tgttgttgga gaagcgggaa atgttagaag accaaattat gtaaaggtca gagctttaaa
    14701 tagaaatggt gaaacaatag agttagaggg agaagaactt ttagctagag cattttgtca
    14761 tgagatagac cacttagatg gaattttgtt tgttgacaaa atagaaaagt agtaggagtt
    14821 gatttgatga aaatagtatt tatgggaact cctgatatag cagttccatg tttacaaaaa
    14881 ataatagatg agaagtatga aatattgggt gttgtaactc aaccagataa accaaaagga
    14941 agaggtaaaa aactaggtat gagtccagta aaagaactgg caattgaaaa taatatacct
    15001 gtatatcaac cagtaaaggc tagagataaa gaatttatag ataaaattaa atctttaaat
    15061 ccagatgtaa tagtagttgt agcttttgga cagatacttc caaaagaaat attagagatt
    15121 cctaaacttg gatgtataaa tgttcatgtt tctttacttc caaaatatag aggtgcggca
    15181 cctataaatt gggtaataat aaatggtgaa gaaaagactg gtgttacaac tatgtatatg
    15241 gatgaagggc tagatactgg agatatgata ctaaagacag aagtaaatct tgatgaaaat
    15301 ataacagcag gagaacttca tgataaaatg atgaatatag gcgcagaaac tttaaaagaa
    15361 acattaagat taattgaaga aggaaatgct ccaagagaag tacaaaatca tgaagaattt
    15421 tcctatgcgc caataatgaa taaatcatta ggtaacatag atttttcaaa aagtgccaga
    15481 gaaatacaca atttagttag aggagtaaat ccatggccaa gtgcctatac tacctataat
    15541 gatgtcataa tgaaagtttg gaaaactaaa gtgttagatg aaaaaagcac aaaagatgta
    15601 ggtacaataa ttgatgtaag caaagatggg ataaaagtaa gtactataga taatgtactt
    15661 ttaattgaag aaattcagat gccgaataaa aagagaatgt tggttggtga atatataaaa
    15721 gggaatacta tagaaactgg tttagtattg ggttaggtga aaagtatgac agatataaag
    15781 aagtatttaa caagtgttgg gattttgatt ttaatattag gaattttgat aggagttgtt
    15841 aattttttta taataagttt aactcaaata ttctccttga tgataaatat tataataata
    15901 tcactaatac ttataatact atgttcaaca gcagttactt atagagtaat taaaggaaag
    15961 tatgttaact caaatataat gaaaataaat tttaatattg tcagtggatt atttcctatt
    16021 atatctttca ttgcatcatc atttggtatg tcaaaaagtg atattagaag aatatatata
    16081 aagttaaata atgaatatat atacagtaat aaatataatt ttaatccaga ggatataata
    16141 attttaatac ctcattgtat tcaagagaat agttgtaaat taaaagttac taatgatata
    16201 gataattgta aagaatgtgg aagatgcaac ataggagaat taattaagct aaaggaaaaa
    16261 actaatgtaa aaatatttgt agctactgga ggaaccctag ctagaaaaat tataatggat
    16321 aataaaccta aagctgttgt agctgttgca tgtgaaagag atttgacatc tggaatacaa
    16381 gatattaaga aaattcctgt tttaggtgta tttaacaaaa gacctaatgg accttgtgta
    16441 aatactaatg tagacattat ggatattgaa aaagctatag gctttttaac aggaaaaaat
    16501 atattagttt gtaattaaac tgtttttaag tgaagtgaat attaagagga tgtataataa
    16561 aaatatattt taggagagtg ttatttatgt accctgtata tggcggtttt tggggatttg
    16621 acccaacaat ggttgtatta attccagcta tattacttac tatatatgca caatttaaag
    16681 tgagttcaac aacaaataag tatttaagag taaatacacg tcgaggatat actggagagc
    16741 aaacagcaag aagagtactt gattcaaatg gtttgtatga tgtaaagata gaaatggtta
    16801 gagggcatct aagtgaccat tatgacccaa gaagaaaggc tgtaaggttg tcagaagatg
    16861 tatattatgg aacatctata acttctgttg cagtagctgc acatgagtgt ggtcatgcca
    16921 tacaacatgc taaaggttat gcacctcttc aaataagaag tagtttagta ccagtagtga
    16981 attttgcttc aagtatatct tggtttttaa tatttttagg atttattatg gctggtccat
    17041 ttttaaaaat tggtatacta ttgttttcgg cttcagtgtt attccaaata ataacattgc
    17101 ctgtagaatt taatgcttca agtagagcaa tcgttcaact tgggaattta gggattatag
    17161 atgaaagtga gtcaagacaa agtagaagag tactttcagc agcagcttta acatatgttg
    17221 cagcagcatt ggtttcaata cttcaattac ttagattgtt actaatagct caaagaagaa
    17281 atgactaaaa gtaaagtggg ttgtttagaa acaacccttt tttatgttta ataaaaggga
    17341 gaaatttata tggatgcaag agaagttgga tttaaagtat tatgtgatat agaaaaaaat
    17401 gacaattatt caaatattgc aataaataag cattttaaaa atttagaaat aagtgatatg
    17461 gatagaggac ttgcaacaga gttaatttat ggagtaatag aaaataaata ttatttagat
    17521 tatataatca ataaactttc aaaaataaag gtaaaaaaaa tgtcaactta tgttaaaatc
    17581 tttttaagaa tgggaacata tcagatttta tttttagata gtatatcaga ttatgcagca
    17641 gttaatgaaa ctgtaaaatt atctaagaaa tatgataaaa aatcttctgg tttcataaat
    17701 gctattctta gaaatgaaat aagagctaaa gagactataa tggacataac tgaggaagat
    17761 agtgtaaaat acttatctat aaagtattcc tataactctt ggattataaa aaactggata
    17821 gataaatttg gacaagaatt tgctgaagat ttgttggaag caaataatga aaagcctagt
    17881 atctatataa gaacgaatac acttaaaata agtagagaag aacttattga aaaattaagt
    17941 gaaatgggta ttgtatgttt aaaagttcct atggtagaag aagctattaa agtcgaaaaa
    18001 atgaaaaata tagaaaaaaa tgaattattt aaggctggtt tatttacaat tcaagatata
    18061 agttctatga tagtagggaa agtaattaat ccaaaagaag gttcatttgt tttggatgta
    18121 tgtagtgctc ctggagggaa atctactcac ttagctactt taatgaacaa tactggtcaa
    18181 gtagtagcta gagatatttt tgagcataaa ctaaagctta taaactctac tgtaaataga
    18241 ttggggctta aaaatgtgtt tgtagaaaga tttgatgctt ctgagattga tgaaaatagt
    18301 ataagtaaat ttgattatgt tttagcagat gttccttgct ctggttttgg tataataaga
    18361 cgtaaaccag aaattaagta taagaaagaa gaagagttaa aagacatcac ttctatacaa
    18421 aagaaaattt tagaaaatgc atctaaatat gtcaaagttg gtggaacttt agtatatagt
    18481 acatgtacaa ttcaagatgt ggagaatata aatatagtta cttcttttgt tgaagaaaat
    18541 cacaactttg aatttgttcc aattgataca gtaaatgtag acttagacaa tcaagataaa
    18601 ggatatttaa agatatatcc taatattcat ggtatagatg gttttttcat agcaaaatta
    18661 aagagaataa ggtagtgaga gaatggaaaa gaaaaagata gtattaaaaa attttactga
    18721 agatgaactt aaagagttta tgaaaactat agatgaaaag ccctttagag gaagtcaaat
    18781 attctcttgg atatataagg gtgcaaaaac ttttgatgat atgaacaata taccaaaaag
    18841 tttgagaaat aaattagaag aggtttcatg tatagggcat atagatatag aattaaagtt
    18901 agaatctaag gtagataata caaaaaaata tttgttttta ttagatgatg gaaatataat
    18961 agaaactgtg atgatggatt atgatagtag agttactgtc tgtgtttcta accaagtagg
    19021 ttgtaggatg ggatgtaatt tttgtgcatc tacaatggat ggattaatta gaaacttaga
    19081 gccatgggaa attttagacc aagttattaa aattcaagaa gatactggta agagggtatc
    19141 aaatcttgta ttgatgggga gtggtgagcc acttgataac tttgaaaata caaaacaatt
    19201 tttaaaaata attaacgaaa aaaatggtct taatataggg tatagacata taactctttc
    19261 aacttgtgga atagtaccta aaatgtatga gttggcagac ttagaaatag ctataaattt
    19321 agctttatca cttcattcac catatgatga agaacgaaga aaaattatgc ctgtagcaaa
    19381 tgcttattct attgaagaaa tattgaatgc atgtagatat tatataaaaa agacaaatag
    19441 aagggttaca tttgaatatt ctcttataaa aggagtaaat gactcagaaa aagaagcaaa
    19501 ggcattagct aagctcctaa aaggtatgct atgtcatgta aatttaattc caataaataa
    19561 agttgaagaa agagaatacg aaaaaccaga taaagcattt atttataaat ttagggatag
    19621 tttggagaag aacaatatac ctgccacagt taggatgtct atgggttctg atattagtgg
    19681 agcttgtggg caattgagaa gaaaatacaa gtaatttgta aggaggaagt cgtatggttt
    19741 atagttgtgc ctcccatata ggaaaaataa gaaaaaacaa tgaagactat tgtgagggag
    19801 aagttataga tacagaacat ggtccaatag gaatatttgc catagctgat ggaatgggtg
    19861 gacataaaaa aggtgaagtc gccagtaaat tagcagtaga aaatataatt gattttctaa
    19921 aggaaaactt attacaacat gataatgtaa aaatagacta tatagatgat atattaaaac
    19981 aggcttataa taatgtaaac tcaattgtgc acaaaaaatc tatggaagat atagaatttg
    20041 aaggaatggg aactacatta gttacggcta tagtgtataa taatgtctta tatgtggcaa
    20101 atgttggaga tagcagatgt tatttgttaa cagatgaaaa atttgataaa ataactatag
    20161 accattccgt ggttgaagaa cttatgatgg cccatgtcat tacagaagaa gaggcaagaa
    20221 gacatccaca gaggaataga ataactagag caattggaac agatgatatg gttgttgtag
    20281 atatttttaa gaaagaaatc aaaaaaagtg atataatact tcttgcgaca gatggattaa
    20341 ctgggtttat tgatgatgaa gatataagag atttaatatt agattatgaa tatgagagaa
    20401 ctagtaatat aagtgaagaa ttgataagta tggccaatga tgtttctggt aaagataatg
    20461 tttcggttat agtgataaaa gtataattag gacggtgata ttgtgggaga tacaatttta
    20521 ggaaatcgat atgaaatcat caggaagatt ggtgatggag gaatggcctt tgtatatgag
    20581 gctaaagaca gattattaaa caggactgtt gcacttaaag tacttagacc tgaatttgta
    20641 gatgatgatg aatttctaac aaagtttaaa agagaggcag aagcagtagc aagtctttca
    20701 catccaaata tagtaaatgt ttatgatgtt ggtgaagatg gaaaagttca ctacattgta
    20761 atggagtttg tagatgggaa gaatctaaaa gagattatac aagatgaagg tatattagat
    20821 gaatatactg cattagatat aacaaaacaa atagctatgg cacttagtgc agcgcacaaa
    20881 aaggggatta tacatagaga tataaaacct cataatattc tcatatcaaa tgaaggaaga
    20941 gtagtaaaag tggctgattt tggtatagcc aaagctgttt caaattcaac aatgactaat
    21001 attggtagta taataggttc agtgcattat ttttcaccag agcaagctaa aggtaaattt
    21061 gtaactaata atgctgacct gtattctcta ggtatagttt tatatgaaat gttgatagga
    21121 aaagttccat ttaggggaga tagtccaatt tctatagcac ttcaacacat taatgatgat
    21181 atagacttta catcagaaga aaaagttagg attcctcaaa gtgtaagaac tacaataaaa
    21241 aagcttacag aaaaatctag tgcagataga tatcaaactg ctgaagaatt gatagaagat
    21301 atagagtata tagaaaaaaa tattgattta gattttataa aagaatatga tgattttgca
    21361 actaagaaaa ttgatgaaaa agaaattaat aaagttgtaa atccaacact tgcaaaacca
    21421 gctcctgaaa aagtggttaa gcctgttgaa gtagctgatt tggatgatga tgaagattac
    21481 tatgatgatt tttatgaaga agatgatgat gaagatgaag aagaggaaga gataatgaga
    21541 gctaaaaaga atcagagacc taagagtact ccaagtaaga gaactaagaa aaagaagaaa
    21601 aaacaggaaa gcccaaaatc tagaagaaga ttaaaagtaa ttgcggctgt tttgatactg
    21661 atattgtgtg ctcaagtatt cttagcatat aagtttttat ttgctggagg gtttggcaat
    21721 aaatctttga ctgttcctaa tcttgtaaat atgacattag aagaagctca aagtgctgtt
    21781 gaaaaagaag gattatacct gagtgtaaaa agtgaagagt ataatagtga agttgatgaa
    21841 aattgtataa tatcacagac acctgaaggt ggaagtacaa atgtcaaaaa gggtgatact
    21901 ataaatgttg tcgtaagtaa aggttcaagt caggcatctg ttcctaatgt agtgggactt
    21961 actttgagta atgcaaaaca acttattgaa gaaaataact taaaagtggg aactgttaag
    22021 tatgaataca gctcaatata taaagaaggt acagttttaa gtcagagtcc gggtgctggc
    22081 tcatcaagag ctcaggaagg tgatgaggta aacctatatg taagcaaagg ttcagaaaaa
    22141 tcaaatacac agactccaac agtaccagat aaaaaaccaa caacaccaga gaataataca
    22201 ccaacagaac ctggttcaaa ctcaggaaac agtggtggaa attcaggagg ctcaaattca
    22261 ggaaatagtg gtggaaactc aggcaataat ggaagtaact caggaggttc aaactcagga
    22321 aatagtggtg gaaactcagg aggctcaaac tcaggagata atggaggaag ttcaggaggc
    22381 tcaaactcag gagacaatgg aggaagttca ggaggttcaa actcaggaga caatggagat
    22441 agttcaggag gctcaaactc aggagataat ggaggaactt catcaggaac tggcgcaaac
    22501 attggtaaaa cagaataaat attaataaaa taagagctgt ccttttatag aaaatatttt
    22561 ctttaaaggg cagtttgttt tgtataaaat actgaattgg ttataatata aggagaaaag
    22621 tatttatgaa gaagaaaaca gttttaatta caggaggagc tagagggatt ggtaaagcta
    22681 tgtctaaagc ttttgcaaaa gaaggttaca atgtattagt aaattttaat aagtcagaaa
    22741 atgaagctaa agagttgtac actatattaa atgaaaaaaa tttttctgta aaacttttta
    22801 aggctgatat ttcaaataga gaagatgtag aggatatggt agattattgt ataaaagaat
    22861 ttggaggatt ggatgtactt gtaaacaatg ctggtgtaag tcaagataag ttgtttacag
    22921 atataactga tgaagattgg gataatatga tgaatattaa tttaaaagga agcttttatt
    22981 gttcacaagt tgctttaaaa tacatgatat cagaaaaaaa aggaaatata attaatatat
    23041 cttcaatctg gggtatatca ggtgcgtctt gtgaagtgca ttattctata acaaaagcag
    23101 gcataatagg tatgacaaag gcattagcta aagaagtagg accatcaaat attagagtaa
    23161 atagcatagc tccaggtgtc attaatactg acatgttatc tggatataat gaggaagata
    23221 tagacgctct tgtggaagaa acccctctaa tgaggctagg aactccagaa gatatagcaa
    23281 attgtgctat tttcttggca tcagacaaat caaattttat cactggtcag gtaattagtc
    23341 caaacggagg atttgttatt taaaaaaaat gtggtattat ataaaaatag gaaagaataa
    23401 cacgatatac tactcataaa cagtatgtgt ttatataaat tttataaagg gaggcatttg
    23461 atgctagaag gaaaaattat aaaggggatt agtggatttt attatgtaga tacttataat
    23521 ggtatttatg aatgtaaggc tagagggata tttagaaagc agaaaataac acctttggtt
    23581 ggtgatagag tcaaaataag cattgttgat gaaaatgaaa aaaagggtat cttagaagaa
    23641 atcgacagta gagatacaga acttataaga cctcctattg caaatgtaga taaagcatta
    23701 atagtattcg caataaaaaa tcctaaacca aacttatctc ttttagatag atttatagtt
    23761 ttggcagaaa aagaaaattt agaaactgta ataatcctta caaaagcaga tttagatgat
    23821 aatgatacat tagaaactgt taaaaatata tatgaactta gtggctataa agtcattcct
    23881 gtaagcaata ttactaagtt aaatatagat aaagtaaaag aagaattaaa agaaaatgta
    23941 gtagtgtttg caggaccatc tggagttgga aaatcaagct tattaaatga aatagatgaa
    24001 aattttaaac ttcaaacagg agttgtaagt gataaaataa aaaggggaaa acatactact
    24061 cgtcacgctg aacttttaaa attagaattt ggtggaatgg ttgcagatac accaggtttt
    24121 agttcccttg cccttgaaga tatagaggaa gttgaactta aagattattt tatagaattt
    24181 gataaattta atgattgtaa gtttggttca aagtgtattc atgaaaatga acctaattgt
    24241 gctataaaag aagctgttac aaatggagaa atatctaaag aaagatatga tagttatata
    24301 cagctgctac atgaaataag acaaaataat agtaggaggt attagaaaca tgattaaatt
    24361 agcaccatca atattatcag cagattttgc aaaattatta gaagatgtaa gaaaagtaga
    24421 aagtgcagga tgtgaatatc ttcatatcga tgtaatggat ggacactttg taccaaacat
    24481 tacattagga ccacttgtag tcaaaagttt aaagaaagaa aatataaata tggtatttga
    24541 tgctcatctt atgatagaaa atccagacca gtatattgaa gaatttgtaa aagcaggatg
    24601 tgatataata actgttcacc aggaggcttg tgttcatcta catagaacta tacaaaatat
    24661 aaaatcacat ggaataaaag ctggtgtagt acttaatcca gctacaccag tagatacgat
    24721 taaacatgta ctacctgatt tagatatggt attattaatg tctgttaacc ctggatttgg
    24781 aggacaatca tttattcctt gtgttttaga taagattaaa gagttaaaag ctattataga
    24841 tagtcaagga ttaaacattg atatagaagt tgatggtggt ataagcccta aaaacgtagc
    24901 cgaagttgta caagcaggag ctaatgtaat agtagcaggt tctgcaatat ttggaagtga
    24961 tgacattcaa gaaacagtta atttatttag aaagaatgct tctcttgaag aattagttta
    25021 aatttaataa tgaaaatctg tattgtttta aatggcgaaa ttgaagatta cgaagttaca
    25081 agagatatta taattaagga atgttatgac tgcataatat gtgcagatgg aggggcaaat
    25141 cacacttata aaatggaaat aatgccagat tatatattag gagatttaga ttcagtagaa
    25201 gaagaaaaga taaacttcta taaaaataaa ggtgttaaat ttgaaaaatt tccatctaaa
    25261 aaagatgaga ctgatacaga gctttgtctt tttttagcta agacacttaa agcaaatcat
    25321 attgattttt ttggtgcttt agggggaagg attgaccaca ctcttgcaaa tataaagcta
    25381 ttatattatt taaaagaaga tggtatatat tctaggatac tttctgataa agaagagatg
    25441 tatatagttg aaaatgagga aatttcattg tatggaaacc caggggacac tatttctgta
    25501 atagctataa atggtgatgc taagggtgta acacttactg gcttagaata ccctctcgat
    25561 aattattata tgaaatactc tgtacctatt ggaatatcaa atgttatgtt gagtaattct
    25621 tgtaagatta aagtagagca aggctgtgta cttgtagtta gaaatttata aaatttgatt
    25681 ttgacaattg gttttatatt gtagagcaga tacaatcaag taaaatatat ttatgattgt
    25741 atctgctttt tagtatataa ttataaatat ataataaact ttgacataag ggggattttt
    25801 tgtggtagat agtattattt ttgatttaga tggaacattg tgggattcaa ctgaagaagt
    25861 atgcaaagtc tggcaggatg ttttaagtga gcataaggaa atagaattaa gtgtcacaaa
    25921 agatttattt agaagtctta tggggctttc ttttgaggaa atagcaaatc gtttatttcc
    25981 aaatttaagt gaagaagaaa gaatgaattt attgaatgaa tgttctttga aagaatgtga
    26041 ctatttatct gaaaatggag ctaaattatt tgatgatata gaagatactc ttaaattact
    26101 atctaaaaaa tataaattat ttatagtaag taattgtcaa gttggatata tagaatcttt
    26161 tttaaaagca cataaactag aacagtattt tatagatttt gaatgtcctg gtaatactgg
    26221 tttgcataaa ggagaaaata ataaacttat aattgaaaga aataaattaa taaatccaat
    26281 ctatgttggt gatacgcaag gtgatgcaaa tagtgctaag tttgtaggta taccttttat
    26341 atatgccaaa tatgggtttg ggaatgtaga tgtatatgat tattttatag attcatttaa
    26401 agatttgtta gaacatgata tattaaaata agatgagttt aaaaattata ttaaaataat
    26461 aaaaaagtta tggggggatt taaaaatgga attacaagat actattttta aaagacaaag
    26521 tgtaagaaag tttaaaaatc aagatgtatc agatgaagat attttaaaaa tgataaaagc
    26581 agcaggtgca gcaccatcag gcaagaatat tcaaaactgg cattttgttg ttataaaacg
    26641 ccgtgattta atggaaaaga tagcagatgt gataactaaa aagcaacaag aaatacttgt
    26701 agaaatggat aaagtatcag tggataaggc taatagattt agaaaatttg ttaaaaattt
    26761 tacactattt tacttaaaag ctccagtttt agtattagtt tttacaaagg tatataatcc
    26821 atcaggatat tatgaattag agcttataga tgcacctaag gagactatag ataaattatt
    26881 tataagaaat ccaggtatgc agagtctagg tgctgcaatt gaaaacttta cactatcagc
    26941 aattgaactt ggatatggtt cttgttggtt gacaagtcaa aactatgcag cagatgagat
    27001 agaagctgtt ttagaagcag aaactggttt tgaaaaggga gagtattttt taggtgcaat
    27061 gttagctctt ggagtgccag aggacaattt gaagagtcca tctaaaaaac cagtagaaga
    27121 aatatgtaca tttataaaat aaaagtcaag gacattttaa aatcgaagta aattaaaaac
    27181 aaaataaatt aaaaataaat atttaaaaaa ttaaaaagta ggttttattg atattaattt
    27241 tatatttcaa atcattga
//
LOCUS       DAELLV010000027        27002 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.027, whole genome shotgun sequence.
ACCESSION   DAELLV010000027 DAELLV010000000
VERSION     DAELLV010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27002
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.027"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     regulatory      186..276
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            688..1005
                     /gene="flgB"
                     /locus_tag="KPD58_000989"
     CDS             688..1005
                     /gene="flgB"
                     /locus_tag="KPD58_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HBG0264772.1"
                     /translation="MNTYDIIKMGLDATNLRSQTIANNIANINTEGYKRHYITFEETL
                     NDAMPKIEEKTDNSKGLRVDGNNVDIEEEKVNQAMATLQYNALISFANGKIAMTKSII
                     SGR"
     gene            1013..1417
                     /gene="flgC"
                     /locus_tag="KPD58_000990"
     CDS             1013..1417
                     /gene="flgC"
                     /locus_tag="KPD58_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HBG0264773.1"
                     /translation="MSVFSGMRISASGLTAESLKMDIISSNVANINATRGPNGEAYRR
                     KVVSFEENYDNKLGMLGVKVTSVEDDKSPLKEVYDPSHPDAKPNGYVEYPNVNILVEM
                     TDLITASRAYDSNVDTLNAQKSMISKALEIGK"
     gene            1427..1744
                     /gene="fliE"
                     /locus_tag="KPD58_000991"
     CDS             1427..1744
                     /gene="fliE"
                     /locus_tag="KPD58_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HBG0264774.1"
                     /translation="MPITQINNLMQGAKLDNLIGEAKPLTLNNKEEKKNFEDVIIDTI
                     DKVNFQQVTADDMKEKFIKGEDVSMHEVMLIGQEAQLSMQYLIEVRNKIHDAYQEMNK
                     VQI"
     gene            1769..3319
                     /gene="fliF"
                     /locus_tag="KPD58_000992"
     CDS             1769..3319
                     /gene="fliF"
                     /locus_tag="KPD58_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HBG0264775.1"
                     /translation="MITSFKNKIKEQVAKSRTKISSLKKGTKIALGIGVIAICLALVF
                     TAISASKNKYAVLFSELDAQDAADISAELESKKIDTKVKGSTIYVPRTEVDKLRLELA
                     PSLTGGSKGFALMDDSSSIGLTDEEFQIKKQRMIQGEIEKTIKSFPQVEDTRVHITFG
                     ESSVFEKEAVPGSAAVYLMLKPGTTLEKNQIKSVMALISGSVTNIPEKNVQVIDNKMN
                     LLSEGILEDENVDENNQPINSGISVDKNKASEQALNKQLEKSILEMLEPIFGKGKVKA
                     TVNADLSFDTAEKTEIKIDPDKVAIKETRSKNSSKSAANNVQGVDANMNNQGNNNGTN
                     TEDSLDESFEYETGKIETHTIVAPGELNRLTASVAIDGKIAKGVQADVEDIVNNAIGM
                     DGARGDTLSVVSMVFDPEGKKEAKQELEEIKKEGFKKNIIKAVIGVVAVIAVGAIGYL
                     IYKKRSEREDMDYEDEDYISKSDSVDRKDNPLLGDDPELTLEEAVKMYAEEKPDQVTE
                     IIKTWLNE"
     gene            3332..4402
                     /gene="fliG"
                     /locus_tag="KPD58_000993"
     CDS             3332..4402
                     /gene="fliG"
                     /locus_tag="KPD58_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HBG0264776.1"
                     /translation="MSTEITNVNAFDLSDINESDKNEKVEGEGLKKAAVLLMTLGPNV
                     SSDIIKSLPDKQVQKIGLEIANISSIASKERREILKEFVEINRAKDYVIEGGLEYARE
                     LLSNALGNVKANKLLEGISINTSTKPFKHIIKLDVKKILEILRNESAQTIAVLLAHME
                     PENGAEVLSYLDFDLQNEVAMRISSMGSISPEVIKVIESSLEDKLHSISGDDFNDYGG
                     VSTLVQILINSDRKTEKNILGTIEEQNPDLAAQIKSHIFVFEDIVKLDDISIQKVLKE
                     VTMKDLVYALKGVDDEISDAIYRNQSSRAADALKEEMEMVGVIKVTQVEASQQKIANI
                     VRKLEREGAITIVRNFGGDFVE"
     gene            4395..5126
                     /locus_tag="KPD58_000994"
     CDS             4395..5126
                     /locus_tag="KPD58_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HBG0264777.1"
                     /translation="MNNKIIKSKKALYKGVLDLTIIDKVRVGESNVNKENDCRFRDEV
                     IKEAIESAEIKKNEILEEAKINAQNIEKQAYEKGYSQGQKNGYEDGYKESYDEYTQKA
                     KDEADFIKNQANLILLQANEKMTEYIKEKNKEIVRLAINMASSVLKKHFEDEESMSDL
                     LKQIIQDYEGSSSFIVRCNSFYKDNIEKEFELFKEETSLKSSVFVIADDSIDKGNAII
                     QKENGRIIVGIDCAMEKLKEELLGE"
     gene            5130..6449
                     /gene="fliI"
                     /locus_tag="KPD58_000995"
     CDS             5130..6449
                     /gene="fliI"
                     /locus_tag="KPD58_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HBG0264778.1"
                     /translation="MYKTDISIDFDKINKKIENLELTISEGRVKKIIGLTVEVEGIKA
                     FVGELCVIYNQVNKPVNCEVVGFKDKEVILMALGELTLIAPGCKVISKGIPLSVMCSD
                     NLLGKVLDGLGNPIDNSDAVLGDRYNLNNEPPDPMKRKKIRNIMETGVRAIDAFTTCG
                     EGQRIGIFAGSGVGKSTTLGMIARNAKADVNVIALIGERGREVLDFIDKDLGEEGMKK
                     SVVVCATSDKAPLVRLKGALTATAIAEYFRDQGKKVILMMDSVTRFAMAQREVGVAIG
                     EPPAQKGYTPSVFAMLPKLMERTGTSDKGSITAFYTVLVDGDDFNEPIADTTRGILDG
                     HIVLSRDLANKNHYPSIDVLNSLSRLMNEIASKEDIKIASFARDMLAEYREAEDLINI
                     GAYASGTNKKIDEAIYYHEHIINFLKQGINEKSSFNETISSLRRVFE"
     gene            6480..6926
                     /gene="fliJ"
                     /locus_tag="KPD58_000996"
     CDS             6480..6926
                     /gene="fliJ"
                     /locus_tag="KPD58_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HBG0264779.1"
                     /translation="MELDKNFKFRLQKVLDLKVKDEEEIKMEFAKIQQKKIDIETNLE
                     NLESNYSKYSISKNNDSIQNQKITINYLLALNNSIMDLSEELDKSTNELEKARKQLIS
                     KQIERKSLEKLKEKKYGQYYKEENLKEQNTNDEFASMSYLRNRQVL"
     gene            6955..8160
                     /locus_tag="KPD58_000997"
     CDS             6955..8160
                     /locus_tag="KPD58_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HBG0264780.1"
                     /translation="MKIQADSYNIIKDIGDRKSSPKNKNIKNNKFEDNLYKATDHKKV
                     KDRREVSKQNISSDKPENSDNECHLDEDKSNHLEDENTYEKDCSKNNEALQQLLNLLK
                     DNYKKDKDINTILNNIEKLSISDELKQELKLNKNILNKIANQNSELNKANINNSNDIE
                     NLDIVKLRELLEDNANKDIEVITKTTENDTKNIDLYSFDSNRMDNLNSNKQKDDSSDI
                     LEKLAGVNGNKSGNFGQVINKNVDMNKIKNDNVNLKQIESNVMDDSIKAIKHMKTNDI
                     QELTIKLRPKELGDMNIQLLKDGESMRAVVTVFNKDVFDSINKNIADLKQHLELTNVN
                     IKDVSVQMHSDNTNTSDTFDKAFEQQNRQNNQENMSKKNTNDKHNNVVIEDDIKENTI
                     DDDRVDLLA"
     gene            8178..8786
                     /locus_tag="KPD58_000998"
     CDS             8178..8786
                     /locus_tag="KPD58_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgD"
                     /protein_id="HBG0264781.1"
                     /translation="MYGIATALAGTKGNYRSNNLSQLERNINDIANKQVRSKGNQTVT
                     DNGTPIIGQGDDADKDLFMKILVAQMGNQDPMNPQDPTEYVSQLAQFATLEKMSAMNE
                     KLDVLLALSNSTLINSALSMATSLIGKEVEVSGEEKEDENIVGVVKSTYIKDGEVHLE
                     LEVDGSDKTVGVKYEKLLKIGEVDENSGEENDSSEHESPEEA"
     gene            8813..9796
                     /locus_tag="KPD58_000999"
     CDS             8813..9796
                     /locus_tag="KPD58_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HBG0264782.1"
                     /translation="MIKAMYSGVSGMKANQTKLDVIGNNVANAGTTSFKKSSARITDS
                     FYQTVLYASAPTAALGGTNLGQVGVGSKISSINKDMVQGNVQPTNRPSDLMIDGDGYL
                     PVVRQGTVMYTRDGSFNLDTGFKAADGALKNIKGGRLVNGDGYMLQGMIYDGEYKADT
                     GEFVKKGDAKGKEPIVIPLQSISPKDGTVQNVLEYNISKDGTVEFLLSDGQRTQFVYD
                     IPDGAKVGDTPAGIDKLKGTIQKVQTYAFQNPAGLDAEGGNLFKPSANSGDPQIAGAT
                     GTIIQGAIEASNVDIAEEFTEMIIASRSFQANSKIISTSDEILQEIINLKR"
     gene            9809..10012
                     /locus_tag="KPD58_001000"
     CDS             9809..10012
                     /locus_tag="KPD58_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HBG0264783.1"
                     /translation="MIRVTDLNGRKYILNSDLIVRIDSVPESRILLTTGEKNLVKESV
                     DEIVDKVIKYKRKINVGYIRSEK"
     gene            10012..10830
                     /locus_tag="KPD58_001001"
     CDS             10012..10830
                     /locus_tag="KPD58_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HBG0264784.1"
                     /translation="MKKTDSLTFIGLIVSTVLVLVGAAKGSSSGLKNFFDVSSILITV
                     LGSFGALMITFTIDDIKLIKNALQYSFKTMSVSKLDLLEQFKTLSKKARKEGLLSIES
                     DVMGIEDPFMKKGLELCIDGLEINEIRSILELEIDSVEDTNNRYSKLFKTWGTFAPAF
                     GMLGTLIGLIQMLADLTSPDLIASGMGKALITTFYGSLLANIALNPIAYNIDEKTEKE
                     IYVKEMMLEGIISIQSGESSIVVEERLATYLSNNEKLEIMKSNKNTERAMSNGA"
     gene            10820..11515
                     /locus_tag="KPD58_001002"
     CDS             10820..11515
                     /locus_tag="KPD58_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBG0264785.1"
                     /translation="MARKKNKKKDEINPDAWLATYADTITLILTFFVLLYATSVVDME
                     KFKNIADALKGQFSGTSIFSTGSSTSEKSPIDSVLVDKNDKSNLEQDLKDKVNNSNLQ
                     NSVTVKDDSRGILLELDDSILFDSGISELKVDSKATLNKVYDMIKMMKNKIVIEGHTD
                     NVPVKSSTHESNWDLSSSRAVSVVRYFVEEKGMDPRLFSATGCGEYQPLVSNETAKGR
                     AENRRVNILITID"
     gene            11527..11970
                     /locus_tag="KPD58_001003"
     CDS             11527..11970
                     /locus_tag="KPD58_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HBG0264786.1"
                     /translation="MFKNKKIIIIILILIIFSAGAGFFILGEGGNKQVDKIAGLFIPT
                     GKGKDEVKTENLDLEEMVLKLADEGVRYLKVSITVSYDSSYKDIEKNTPLIRDNIINC
                     FMAKRADDFTADNLKNIKEEIKNTLNIQLGENVIQNIYFTNIVVQ"
     gene            11985..12359
                     /locus_tag="KPD58_001004"
     CDS             11985..12359
                     /locus_tag="KPD58_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliZ"
                     /protein_id="HBG0264787.1"
                     /translation="MILYLLKILICIPIILILIVLSLKISQGTLLKKYNDKYVKILDI
                     VNISKNNSIIILKIGEEGCIMASSSSSMSKIKDLTKEEILKIENDIKERNFKIQDFKM
                     ERIKKKYDFSRYIGKFTKKEDK"
     gene            12360..13025
                     /gene="fliP"
                     /locus_tag="KPD58_001005"
     CDS             12360..13025
                     /gene="fliP"
                     /locus_tag="KPD58_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425215.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HBG0264788.1"
                     /translation="MELLSKLPLADVPYTSSMQLFLFLTALMFLPFVMFMMTSFVRIV
                     ISLSFLKSALGAQQAIPSQILVGLAIVLTIFIMRPVLNEINEKALQPYMKEEVTMEEA
                     MKEAEGPIKEFLLTQTRQTDLDLFVEQAGLKEKKLTRENIPLSVVVPAFAISELKTAF
                     QIGFLIYIPFLIIDLVVASVLMSMGMFMLPPVMISLPFKLLLFVMVDGWNLIVKTLIL
                     GFG"
     gene            13038..13307
                     /gene="fliQ"
                     /locus_tag="KPD58_001006"
     CDS             13038..13307
                     /gene="fliQ"
                     /locus_tag="KPD58_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HBG0264789.1"
                     /translation="MSEFMIMSVLKNGMFTGAMIAAPVLIVSLVVGLLISVFQATTQI
                     QEQTLTFVPKLIAILLVVAIGGSWMLHTLISFTSEIFNMISLITK"
     gene            13319..15133
                     /locus_tag="KPD58_001007"
     CDS             13319..15133
                     /locus_tag="KPD58_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused FliR family export protein/FlhB family
                     type III secretion system protein"
                     /protein_id="HBG0264790.1"
                     /translation="MIFVFIRIVAFFGTLRILFPAGTPALFKSLFAIIISILISSTMQ
                     IEYATNIDNIVLFTLYGINETITGIMLGYITNLCFYSIRMAGSMMDQQMGLSMINMFD
                     PNSMTQTTLIDNLMNWTALMIFFSMDGHHVLIRGIRYSFELIPIGKPFVDNNIDYIIN
                     IFVQCFLTGFKIAIPIVLCLLMADFILGLISRSVPQLNVMIVGMPLKILVGIALFIIS
                     IPLIVNQISHLLSQIPKMYEGTFALAPMFFMGSTDKTEEATPKKKGEQRKKGNIAKSR
                     ELPVAMTLLAFTLLVPTLFSYVVDTLKSSLNYFLSLDFYMNINYSNLEKLVIAGLMDF
                     FKIFLPIAIPFLVLGIIANLFQVGILFTGETLKPNLSKLNPVSGFKNMFSMRSLSTLI
                     KDIAIISILAYIGYTFFQDNYLDILKLGNIYLPTLMYTVKDLVYSILSKICVAMIAIA
                     VADYVYQRYSHKKQLRMTKQEVKDEYKNSEGDPEVKAKIKQKQRQISSQRTMQAVPSA
                     TVIVTNPTHLSIAVRYEKGKDQAPVVVAKGADYLAFKIREIAKGNDIPIIENKPIARL
                     LYKQVEIDQEIPEDMYQAFAEILVAVYKIKNRYKVPKR"
     gene            15142..17217
                     /gene="flhA"
                     /locus_tag="KPD58_001008"
     CDS             15142..17217
                     /gene="flhA"
                     /locus_tag="KPD58_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HBG0264791.1"
                     /translation="MNKFNLKENMDVIVAFGVVGIVLMIIVPLPTFILDILLAINISL
                     SVLILLMTLFTTNVLDLSIFPTVLLVTTLFRLGLNLSSTRLILTQGYAGEVIEAFGSF
                     VVGGNYVVGIIIFLIILIVQFVVITNGSGRVSEVTARFTLDAMPGKQMSIDADLNAGV
                     IDDKTARKRRKELQQEADFYGSMDGANKFVKGDAIAGLIVTAINIIGGIVIGAVMLNM
                     TLMGAAQTYVRLTVGDGLVSQIPALLISTSAGIIVTRASTDENFGGLVGKQLTAIPKA
                     VMMTGCVLIVLGMMPGLPKPAFFSLGIGAIAAGYFLGREKKDTLEDLEFEKPDMVNPD
                     KNEVEDVTNLINVESIEVEIGYGLISLADESAGGDLLQRIASIRRQCAIDMGIVVQPI
                     RIRDNLQLNPNQYNIKIKGNVVGGYEIMPNMVLCMDPMNQGFSIFGIKTIEPTFGIEA
                     LWIESDKVEDAELKGYTVVDSSTILITHLLDIIKSNAHELLGRQEVKTIIDAARENYS
                     AVVDELIPDLMTLGEIQKVLKNLLREKVNIKDRVTILETLADQSRNTKDIELLTEYVR
                     IALARSICNNLVDDDKTIVVATLSPETEELISNNLQRSVNGTYPAVDPENTNKIFESI
                     QEVMSNVYFNHNIPVIVVSPKIRAPFRKLVEIVYPNLTVLSLNEIPNDIKIKAEGVVS
                     IYDDEKVYS"
     gene            17192..18208
                     /gene="flhF"
                     /locus_tag="KPD58_001009"
     CDS             17192..18208
                     /gene="flhF"
                     /locus_tag="KPD58_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HBG0264792.1"
                     /translation="MMTKKYTANTIQDAMNLAKMELGDNITLIDKKEVRKSGIQGIFS
                     KKDIELTIGWEKRENIEQKDLKREIEQLKLIINNMGFDNKNDNDIDKICKNLLSLELN
                     EEIVESIRIDLQEMKFNGIDTSKNLVEILKKKIKIENQVINGKIALVGPPGVGKTTTI
                     AKLAAKLVFEENKKVGVITIDTYRIGAVEQLKIYTDIMNIPFKGVISPDEMELALDEM
                     KDCDVVLIDTTGRGYKNSMQILEIKNLIDKAETDNIHLVLNCTTRESDTKAIIDSYRN
                     VNFKSLIITKLDETITYGSIFNIMNYAQKPISYITTGQNVPDDIIKPNEDKIIRLLLG
                     VESI"
     gene            18205..19083
                     /locus_tag="KPD58_001010"
     CDS             18205..19083
                     /locus_tag="KPD58_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HBG0264793.1"
                     /translation="MIDQAQILRKMAMEKRGLDEFIVENENVPKIITIASGKGGVGKS
                     NLATNLSICLTKLNKKVLILDADIGMSNIDIIMGVNVKGTIIDVINGEKNIEDIISQT
                     KYGVNIISGGSALNHIEDFTEAQRNKFIHSIEQIHDVDFIIIDTGAGMSKSLLSFIYC
                     STEFFLITTPEPTSLTDAYSLLKAISNFGIKRSANIIINRVIDLEEAKSTYKRIKMVV
                     DKFLNLNLELYGYIIDDKKVSLCVKKQIPFILEYPTSIASKCIIAIAKRMVSQSREDI
                     VDNKSFLRKMFSIFKS"
     gene            19097..19798
                     /locus_tag="KPD58_001011"
     CDS             19097..19798
                     /locus_tag="KPD58_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HBG0264794.1"
                     /translation="MNREELIKENMPLVKSIARKFFIPGKGFEYQDLVNSGVIGLIDA
                     INKFDAEKGAKFSSYSYIKIKSAILDEIRNQSPISKHNLTKVNKYNRVVEKLQSKLLR
                     EPTSYEIAKELKVSEKELHDIESNIDMLNIVSLNYVIFEDTNETVQDVISDREEEAPE
                     NIIEEEEKLEILSKAISNLNEREKLILSLYYYEDLNLKEIGKVLGVSESRVSQLHRKS
                     IRNLRNKIKELKYSI"
     gene            19814..20236
                     /locus_tag="KPD58_001012"
     CDS             19814..20236
                     /locus_tag="KPD58_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HBG0264795.1"
                     /translation="MIQIILIVVSILIIVTIVINLHKESKNKDNRYFDKIFEQEYEAN
                     DRLIIAEKRRFFLDKVLAEKRINSHIKVYENYNLIKANEERKENIFIESKKEVRQENE
                     LSLKVKHLISSGYDDVEICKMLDIGKGELLLIRSCYKI"
     gene            20221..20406
                     /locus_tag="KPD58_001013"
     CDS             20221..20406
                     /locus_tag="KPD58_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HBG0264796.1"
                     /translation="MLQNIRILLCDKKVFLGIGIGILISTLCIMLFSNNDMSKAEIEK
                     KARALGMKYTSEMKVLE"
     gene            20447..21220
                     /gene="flgG"
                     /locus_tag="KPD58_001014"
     CDS             20447..21220
                     /gene="flgG"
                     /locus_tag="KPD58_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HBG0264797.1"
                     /translation="MIRGLYTAVSAMITNQQKQNVVVNNLANMDTNGYKSKQLLTKSF
                     DELKLEGYNNYANGTKQKQIIGDISPGISMDETITNFNQGPIKTTDNKMDVAIQGKGF
                     FLVSDAAGNQFYTRNGNFRTDNQGDLITSEGYYVLGTNTATGAVGHINVGNQKFEVAK
                     DNTISLDGNAMYKFNIVDFNNYNNLKKEGDNLYSGGGAMKVNNALTIQSAIESSNVDM
                     VSEINNMMTISREYEANQKIIQAMDSKLAKIASEIGSVR"
     gene            21241..22002
                     /gene="flgG"
                     /locus_tag="KPD58_001015"
     CDS             21241..22002
                     /gene="flgG"
                     /locus_tag="KPD58_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HBG0264798.1"
                     /translation="MYTMFYTSKTALSANQSKLDIISNNIANSDSTGYKKINMEYSDL
                     VDEVLNRPSYPTNGNDISTGTGAKATAPMRNYEQGALAPTDSKSNLAIDGEGFFRVIR
                     KDGTYAYTRNGGFNVDALGKIVDDNGNILDVQFDAGYNYNNTNIDSTNLTVSRNGELF
                     VDNKKIGKINLYQPIGTQNFISEGDSLFVATDAAQIKQVEKVDIVQGYRERSNVSLQE
                     EFVDLIATQRAFQMNSKGIKTADEMWQIANNLRAK"
     gene            22025..22906
                     /locus_tag="KPD58_001016"
     CDS             22025..22906
                     /locus_tag="KPD58_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBG0264799.1"
                     /translation="MNDMKEYDFGKPQSYSRENLTCLNFLLAEFCKKYKNYIIYELKC
                     NSSMVVDSIDQVNYQTFLDRVTSSCVVVQNAMEPVMKNFSFRIDRSVADMWIDITSGG
                     TGVVKDNDRELTELDKKVLLHLMDDLVRNMHLFEGFENIQVLDTHTHINLPQLCSPTA
                     PVCVVDLKVLNDNEYIGSVSLCFPYNGLEALLESISVIEFFDNDVGDDSEEFTQKIYN
                     SVSNIELSVIAEIGKVSINVEDLLKLEVGDVIVTNKKINDYIDIFVENSKSYTATPGF
                     ISSKKGVKINDAVGKEV"
     gene            22909..23283
                     /locus_tag="KPD58_001017"
     CDS             22909..23283
                     /locus_tag="KPD58_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBG0264800.1"
                     /translation="MDNSNLISDEEAKMLLNDKVESEVDGDDELALDVSEVNRNNIQR
                     ILDLKLELSVVIGRTKMTLRDILNLHKGSLIELDTLADQDVEILIDNKVLAYGKVVVV
                     DLNFGVKITSIVSEEDMVKSLV"
     gene            23315..24718
                     /locus_tag="KPD58_001018"
     CDS             23315..24718
                     /locus_tag="KPD58_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG0264801.1"
                     /translation="MVLILNLNDIKKDILKLNKKDSCSLYNESINNINNNCISTGLKF
                     LKQARDLNPDDVDILNLTGLVNLLKCNFDDAVESFYKSSLCEKTALCEKYINMLTSEE
                     FLIFFEKYNQLIRLINKGMYKEAIQGFKLITDQYCDLIEPYELLALLYDKEEEYIKFD
                     ECLEVLKIIDKENYLLNQENNTLYMYNSANIQTKSNIKVDDYKNEFLHKSSSNHKIND
                     YNDVTKEKSSSKNQKSSDSNKKKSIKKNKYLYIAIILGILLVGQSIYSSYKMNNLTDK
                     ISKNNETKNEESIDSNLTKKENNIEENEDMSKASNINKSNKNINKEDDVKSKKNTVEN
                     EDDKDSDLFTEDELMSKANTLKSEKKTKSSIQLYKKVADIGENKGNTSEATYQVAILS
                     EKLKDYKTAEEYYKMYVENYSEKDAYFDESYYNLGMMYYNNGDLKNSKLTLKKLVNKV
                     PNSMYNNSKVKEILKEE"
     gene            24834..26771
                     /gene="htpG"
                     /locus_tag="KPD58_001019"
     CDS             24834..26771
                     /gene="htpG"
                     /locus_tag="KPD58_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HBG0264802.1"
                     /translation="MEFEKGSISIHTENIFPIIKKWLYSDKDIFIRELISNGCDAVSK
                     HKRLVSLGEISENKSSDYKITVSVNKGEGTLKFIDNGIGMTEEEIKKYINQVAFSGAE
                     DFFNKYKDKMEESNDIIGHFGLGFYSAFMVSKKVQIDTLSYTEGATPVRWISEGGTEY
                     EISESDARNDRGTTITLFIDDDSKEFLDEFTVRGIINKYCSFLPVEIYLEDVERLERE
                     AKEAEEKAKKQKEDGKEEIVDAKVIEPLNDTNPLWLKSPKDCTDEEYKEFYRKVFNVF
                     DEPLFWIHLNVDYPFNLKGILYFPKLKNEFELTEGKVKLYNNQVFVADNIKEVIPEFL
                     LLLKGVIDCPDLPLNVSRSFLQNDRDVSKISKHIIKKVADKLKSLCKNEREEYNKFWD
                     DIQIFIKYGCLKDESFYEKVKECILFKTIDDEYITLQDYLEKCKDKHENKVFYVSDKE
                     QQSQYIKLFKEYDLSAVVLNSSIDTHFISFMEYKENGVKFNRIDADLSDVLKDKNENK
                     DSEENKEEIAKIEGLFKEAVGERVKNYSVEGLKNEDTPAMVLVSEQSIRMAEMQSRFA
                     GMDLGMNFEEEKTLVINENSPIIKKLVSLKDDEEKKDKITLICNQIADLALLSNKELK
                     PDELDSFVQRSNKLMSLLIEL"
BASE COUNT        11174 a         2845 c         5013 g         7970 t
ORIGIN      
        1 ttaagatgag atttgtctta tatttttata aattaaaaat attttcaaag agtttattat
       61 aaaattgtaa actatagtgt ttataatctt tgtaattatt ggtaattaaa tttaaaaaat
      121 aaaatattta attatacaca aaaaagatat ttaaaaaatt tgttatttat tgtatactta
      181 agttaaaact aaataggcaa atctagagaa atctagtgac gcaaagctat agggactaag
      241 gtttatatac ataaactatg tcagccagtt gccaaaaaga gtcctaggtg tattgtatac
      301 ctaagaaaag tctataatga ctgccttttt ggcagtcatt ttgttatgga aaaatcactt
      361 tatttcagta gaaaatacac ataataacat attttaccaa ttataacata aaataaagaa
      421 aaattatctc aaaaagttac tatttttaca aaaaaatgac ttctttattt atttaaatta
      481 atcgcatttc cattacatta atacaataat gtatcaattt agtaagaaaa ctaatatttt
      541 tttaatgaag tcccatgaaa atataaaaaa attttaatat gtatttaaat atttcttcga
      601 aaagttgctc tttttttgta atatagcaac ttcatttttt ttattaataa acaaaataga
      661 taaaatatat tttgaggagg ggtgagtgtg aatacatatg acattataaa aatgggatta
      721 gatgcaacta atctaagaag tcagacaata gcaaataata tagcaaatat aaatactgaa
      781 ggatataaaa gacattatat aacttttgaa gaaacactaa atgatgctat gcctaaaata
      841 gaagaaaaaa cagacaattc taaaggttta agagtagatg gtaataatgt agacatagaa
      901 gaagaaaaag taaatcaagc aatggcaaca ttacaatata atgctttgat aagttttgca
      961 aatggaaaaa tagcaatgac caaatcaata ataagtggga ggtaattaga agatgagtgt
     1021 gttcagtgga atgagaatat ctgctagtgg attaacagca gaaagtttga aaatggatat
     1081 catatcatcc aatgtagcta atataaatgc tacaagaggt ccaaatggag aagcatatag
     1141 gagaaaagtt gtttcatttg aagaaaacta tgataataaa ttaggtatgc taggagtaaa
     1201 agttacatct gttgaagatg ataaatcacc tcttaaagaa gtatatgacc catctcatcc
     1261 agatgcaaag ccaaatggat atgtagaata tccaaatgta aatatattgg tagaaatgac
     1321 agatttaata acagcttcac gagcatatga ttctaacgta gatacattga atgctcagaa
     1381 gtctatgata tcaaaagctt tggaaatagg aaagtaggga taaattatgc caattacaca
     1441 aattaacaat ttaatgcaag gtgcaaagtt agataatttg attggagaag caaagccatt
     1501 aactcttaat aataaagaag aaaaaaagaa ttttgaagat gtaataatag atactataga
     1561 taaagttaat tttcaacaag taacagcaga tgatatgaaa gaaaaattta taaaaggcga
     1621 agatgtttct atgcatgaag ttatgttaat aggtcaagaa gcacaactat ctatgcaata
     1681 tttaatagaa gttagaaata agattcatga tgcatatcaa gaaatgaata aggtgcaaat
     1741 ttagcctttt gaacaggagt aataatcaat gataaccagt tttaaaaata agattaagga
     1801 gcaagttgct aaatctagga caaagataag ttctttaaaa aagggaacta aaatagcatt
     1861 aggtataggg gttattgcta tatgtttagc gctagtattt acagcaatat ctgcgagcaa
     1921 aaataagtat gcagtcttat tttcagaact agatgctcaa gatgctgcag atatatcagc
     1981 agagctagag tctaaaaaga tagatactaa ggtaaaagga agtacgattt atgtacctag
     2041 aacagaagtt gataagttaa ggctggagtt agcgccaagt cttacaggtg gttctaaagg
     2101 atttgcacta atggatgata gttcatcaat agggcttact gatgaagaat ttcagattaa
     2161 gaaacaaaga atgatacaag gtgaaataga aaaaacaata aagagttttc cacaagtgga
     2221 agacactaga gtacatataa catttggaga gtcatcagta tttgagaagg aggctgttcc
     2281 agggagtgca gcagtttatt tgatgctaaa accagggacg actttagaaa aaaaccaaat
     2341 aaagtctgtt atggcactta tatcaggaag cgttacaaat ataccagaga aaaatgttca
     2401 ggttattgat aacaaaatga atttattatc tgaaggtatt ttggaggatg aaaatgttga
     2461 tgaaaataat caaccaataa atagtggcat tagtgttgat aaaaataagg catctgaaca
     2521 agcattgaat aaacaactag aaaagtctat tttagaaatg ctagaaccga tatttggtaa
     2581 gggaaaggtg aaagctacag taaatgctga tttaagtttt gatacagctg aaaaaactga
     2641 gataaaaatt gaccctgata aagttgcaat aaaggagaca aggtcgaaaa attcatctaa
     2701 atctgctgca aataatgttc aaggtgtaga tgctaacatg aataaccaag gaaataacaa
     2761 tggtactaat acagaagata gtttagatga gagttttgag tatgaaacag gtaagattga
     2821 aactcacact atagttgcgc ctggagaact taatagattg acagcttctg ttgcaataga
     2881 tggaaaaata gctaaaggtg ttcaagctga tgtagaggat attgttaata atgctatagg
     2941 tatggatgga gcgaggggag atactcttag cgttgtttca atggtatttg acccagaagg
     3001 taagaaagag gctaaacaag agctggaaga gattaaaaaa gaaggattta aaaagaacat
     3061 aataaaagca gttattggag tagtagcagt aatagcagtt ggagctattg gatacttaat
     3121 atataagaaa agaagtgaac gagaagatat ggattatgaa gatgaagatt atatttctaa
     3181 atctgattct gttgatagga aggataatcc tctattagga gatgatccag aactgacatt
     3241 agaggaagct gttaaaatgt atgcagaaga aaagccagac caagtaacag agataataaa
     3301 gacttggtta aatgagtaag ggggatagaa agtgtccacg gaaattacaa atgttaatgc
     3361 gtttgactta agtgatataa atgaaagtga taagaatgag aaggtagaag gtgagggttt
     3421 aaaaaaagct gctgtattat taatgacatt aggcccaaat gtgtctagtg atataataaa
     3481 aagcttgcct gataaacaag ttcaaaaaat aggtttggaa attgcaaata tttcttcaat
     3541 agcttcaaaa gaaagaagag aaatcttaaa agagtttgta gaaataaata gggcaaaaga
     3601 ttatgttata gaaggtgggc ttgaatatgc aagagagtta ttaagtaatg ctttaggaaa
     3661 tgttaaagca aacaaattac ttgaaggtat atcaataaat acaagtacaa agccttttaa
     3721 acatattata aaactagatg taaagaagat attagaaata cttaggaatg aaagtgcaca
     3781 aactatagcc gttttacttg cacatatgga accagaaaat ggagcagaag ttttatcata
     3841 cttagatttt gatttacaaa atgaagtagc tatgagaata agctcaatgg gttcaatatc
     3901 tccagaagta ataaaggtaa ttgaaagttc attagaagat aaattacatt caataagtgg
     3961 tgatgatttt aatgattatg gaggggtgtc taccttagtt caaatattga ttaattctga
     4021 tagaaaaaca gaaaagaata ttttgggaac tattgaagaa caaaacccgg atttagcagc
     4081 tcaaattaaa tctcatatat ttgtctttga agatattgtc aagttagatg atataagcat
     4141 acaaaaagtg ttaaaagaag tgactatgaa agatttagta tatgccttaa aaggtgtaga
     4201 tgatgagatt tcagatgcta tatatagaaa tcaatcttct agagcagcag atgcacttaa
     4261 agaagaaatg gaaatggttg gtgttataaa ggttacacaa gttgaggctt ctcaacaaaa
     4321 gatagctaat atcgtaagaa aacttgaaag agaaggtgcc ataactattg taaggaattt
     4381 tggtggtgac tttgttgaat aataagatta ttaaaagtaa aaaagcatta tataaaggag
     4441 tgctagattt aaccataata gataaagtta gagtgggaga atcaaatgta aataaagaaa
     4501 atgattgtag atttagggat gaggtaataa aagaagctat agaatctgct gagataaaaa
     4561 agaatgagat attagaagaa gcaaaaataa atgcacaaaa tatagaaaag caagcatatg
     4621 aaaaagggta tagtcaaggt caaaaaaatg gatatgagga tggatataaa gagtcatatg
     4681 atgaatatac ccaaaaagct aaagatgaag cagattttat aaaaaatcag gcaaatttaa
     4741 tcttattaca agctaatgag aagatgactg agtacataaa agaaaaaaat aaagagattg
     4801 taagacttgc tattaatatg gccagttctg ttttaaaaaa gcatttcgaa gatgaagaat
     4861 ctatgagtga tctgttaaag caaattattc aagattatga aggaagtagt tcatttatag
     4921 taagatgtaa ttctttctat aaagataata tagagaaaga gtttgaatta ttcaaagagg
     4981 aaacatcact aaaatcaagt gtatttgtta ttgcagatga tagtatagat aaaggcaatg
     5041 ctattatcca aaaggaaaat ggaagaataa tagttggtat tgattgtgct atggaaaaac
     5101 taaaagaaga attgcttggt gaataaagta tgtataaaac tgatatatct atagattttg
     5161 acaaaataaa taagaaaata gaaaatttag aactcactat atcagaaggt agagtaaaga
     5221 agataatagg acttactgta gaagttgaag gaataaaagc ttttgttggg gagttatgcg
     5281 ttatatacaa ccaagttaat aaaccagtga attgtgaagt agtaggtttt aaagataaag
     5341 aagtcatatt aatggcgttg ggagaattaa cattgatagc acctggttgt aaagttattt
     5401 ctaaggggat acctttaagt gttatgtgta gtgataattt actaggtaaa gtacttgatg
     5461 gtcttggaaa tcctatagac aactcagatg ctgttttagg agatagatac aacctgaaca
     5521 atgaacctcc tgatccaatg aagagaaaaa agatacgaaa tataatggaa acaggtgtaa
     5581 gagcaataga tgcatttact acttgtggtg aaggacagag aatcggcata tttgcaggaa
     5641 gtggagtagg taagagtacg actctaggta tgattgctag gaatgcaaaa gcagatgtaa
     5701 atgtaattgc tcttattgga gaaagaggaa gagaagtact agattttatt gataaagatt
     5761 taggtgaaga gggaatgaaa aaatctgttg ttgtgtgtgc aacatcagat aaagcccctc
     5821 tagttaggtt aaaaggtgct cttactgcta ctgcaatagc agaatatttt agagaccaag
     5881 gcaaaaaagt aatacttatg atggattcag taactagatt tgctatggca caaagagaag
     5941 ttggtgtagc aataggagag ccaccagctc aaaaaggata tacaccatct gtatttgcaa
     6001 tgttgccaaa acttatggag agaacaggga catcagataa aggttctata actgcatttt
     6061 atacagtatt agttgatgga gatgacttta atgagccaat agcagataca acaagaggga
     6121 tacttgatgg tcatatagtt ttatctagag acttggcaaa taagaatcac tatccttcta
     6181 tagatgtatt aaacagttta agtaggctta tgaatgaaat tgcttcaaaa gaagacataa
     6241 aaatagcgtc ttttgcaaga gacatgcttg ctgagtatag agaagcagaa gatttgataa
     6301 atataggtgc atatgcttca ggtacaaata aaaaaataga tgaagcaatc tattatcatg
     6361 agcatattat taatttttta aaacaaggga taaatgaaaa gagttctttt aatgaaacta
     6421 taagtagttt gagaagagtt tttgaataaa caattaaaaa aatatttgaa taggttgtga
     6481 tggaattgga taaaaacttt aagtttagat tacagaaagt tttagactta aaagtgaaag
     6541 atgaagaaga gataaaaatg gaatttgcta agattcaaca aaaaaaaata gatatagaga
     6601 ctaatttaga aaacttagaa agtaattata gcaaatattc tataagtaaa aataacgata
     6661 gtattcaaaa tcaaaaaata actattaact atttactagc tttaaacaat tctataatgg
     6721 atttatcaga agagttggat aagtcaacaa atgaacttga aaaagctaga aagcagctta
     6781 taagcaaaca gatagagaga aaatcattag aaaaattaaa agaaaaaaag tatggacagt
     6841 attataaaga agaaaattta aaagaacaaa acactaatga tgagtttgca agtatgagtt
     6901 atttaaggaa tagacaagtt ctataagaat agtaggaagt gaggatatta ttgtatgaag
     6961 atacaagcag attcttataa tattattaaa gatattgggg atagaaaaag ttcacctaag
     7021 aacaaaaata taaaaaataa taaatttgaa gataatctat ataaggcaac agaccataaa
     7081 aaagttaaag atagaaggga agtaagtaag caaaatatta gctcagataa accggaaaat
     7141 agtgataatg agtgtcattt agatgaagat aaaagtaatc acttagaaga tgaaaataca
     7201 tatgaaaaag attgtagtaa gaataatgaa gctttgcagc aattactgaa tcttttaaag
     7261 gataattata aaaaagataa agatataaac actatattga ataatataga aaaactaagt
     7321 atatcagatg aattaaaaca agaacttaag cttaataaaa atatattaaa taagattgca
     7381 aatcaaaatt cagagctaaa taaagctaat ataaataact ctaatgatat agaaaattta
     7441 gacatagtta aattaagaga acttttggaa gataatgcta ataaagatat tgaagtaatt
     7501 actaaaacta cagaaaatga taccaaaaat atagatttgt atagttttga tagcaataga
     7561 atggataatc ttaactcaaa taaacagaaa gatgatagct ctgatatttt ggaaaaacta
     7621 gcaggggtaa atggaaataa gagtgggaat tttggacaag taataaataa aaatgttgat
     7681 atgaataaaa ttaaaaatga taatgtcaat ttaaaacaga tagaatcaaa tgtaatggat
     7741 gactctataa aagctattaa gcatatgaaa acaaatgata tacaagaatt gacaattaaa
     7801 ttaagaccaa aagaattagg agatatgaat atacaattgt taaaagatgg tgaaagtatg
     7861 agagctgttg tgactgtttt caataaagat gtctttgact ctataaataa aaacatagca
     7921 gatttaaagc aacatcttga acttactaat gtaaatataa aagatgtatc agtgcaaatg
     7981 cacagcgata atacaaatac atctgatacc tttgataaag catttgaaca acagaataga
     8041 caaaataacc aagaaaacat gagtaagaag aatacaaatg ataagcataa taatgtagtc
     8101 atagaagatg acattaaaga aaatactatt gatgatgata gagtagattt actagcttag
     8161 taagggggaa gtattgcatg tacggaatag caacggcttt ggcaggaact aagggaaact
     8221 ataggtcaaa taacttaagc caacttgaaa gaaatattaa tgatattgcc aataaacaag
     8281 ttagaagcaa aggaaatcag acagtaacag acaatgggac tccaattata ggacaaggtg
     8341 atgatgctga taaagacttg tttatgaaga tacttgtggc acagatgggt aatcaagacc
     8401 caatgaatcc gcaagaccca acagaatatg tgtcacaatt agcacagttt gcaactttag
     8461 aaaagatgtc agctatgaat gagaagctag atgtactttt agccttaagt aattccactc
     8521 taataaactc tgctttaagt atggcaacat cattaattgg taaagaagta gaagtatctg
     8581 gggaagagaa agaagatgaa aacattgtag gagtagtaaa gtccacatat ataaaagatg
     8641 gggaagtaca cttagaatta gaagtagatg gaagtgacaa aactgtaggt gttaaatatg
     8701 aaaaattact taaaatagga gaggtagatg aaaattcagg agaagaaaat gattcttcag
     8761 aacacgaatc tccagaagaa gcataatatt aaatgagaaa agaggtaaat tcatgataaa
     8821 agcaatgtat tcaggtgtaa gtggaatgaa agcaaatcaa acaaaattag atgtaatagg
     8881 taacaatgta gccaacgctg gaactacttc ttttaagaaa agttcagcta gaataacaga
     8941 ttcattttat caaactgtac tatatgcaag tgctccaaca gctgcattag gaggaacaaa
     9001 cttaggacaa gtaggtgtag gtagtaagat atcaagtata aataaagata tggtacaagg
     9061 aaatgtacaa ccaactaata gaccatctga tttaatgata gatggagatg gatatcttcc
     9121 agtagtaaga caaggaacag ttatgtatac gagagatggt tcttttaatt tagatacagg
     9181 atttaaagca gctgatggtg ctttgaagaa tattaaaggt ggaagacttg taaatggtga
     9241 tggatatatg ttacaaggta tgatttatga tggggaatat aaagctgata ctggagaatt
     9301 tgttaaaaag ggtgatgcta aaggaaaaga gcctattgta ataccacttc aaagtataag
     9361 tcctaaggat ggaacagttc aaaatgtact agagtataat atatcaaagg atggaacagt
     9421 agaattttta ttgagtgatg gtcaaagaac tcaatttgtc tatgatatac ctgatggtgc
     9481 taaggtagga gatacacctg ctggaataga taaattaaaa ggtacaatac aaaaagtgca
     9541 aacatatgca ttccaaaatc cagcaggact agatgcagaa ggaggaaact tgtttaaacc
     9601 atcagcaaac tcaggagacc cacaaatagc aggagctaca ggaacaataa tacaaggggc
     9661 aatagaagct tccaatgtag atatagcaga agaatttaca gaaatgataa tagcaagtag
     9721 gtctttccaa gcaaacagta aaataatatc gacatcagac gagatacttc aagaaataat
     9781 aaatcttaag agatagtagg tacatattat gataagagta acggatttaa acggaagaaa
     9841 atacatatta aattcagacc ttatagtgag gattgattct gtaccagaaa gtaggattct
     9901 ccttacaaca ggagaaaaaa accttgtaaa agagtcggta gatgagatag tagataaagt
     9961 aataaagtat aaaagaaaaa taaatgtagg ttatattagg agcgaaaaat aatgaaaaaa
    10021 acagattcac tgacatttat tggactaata gtatctacag tattagtttt agttggtgcc
    10081 gcaaaaggaa gtagttcagg gttaaagaat ttttttgatg tttcatcaat attaatcaca
    10141 gtgcttggtt catttggagc tctaatgatt acatttacta tagatgatat aaaacttata
    10201 aaaaatgcat tacaatactc atttaagaca atgagtgtat caaagttaga tttactagaa
    10261 caatttaaaa cactttctaa aaaagctaga aaagaaggtc ttttatcaat agagagtgat
    10321 gtaatgggaa tagaagatcc atttatgaag aaagggttag agctgtgtat tgatggattg
    10381 gaaatcaatg agataaggtc catcttagaa ctagagatag attctgttga agatacaaat
    10441 aacagatact ctaaactatt taaaacttgg ggaacctttg cacctgcttt tgggatgttg
    10501 ggaacactaa taggtttaat acaaatgtta gctgacctta catctccaga tttaatagca
    10561 tctggcatgg gaaaggcatt aattacaacg ttttatggtt ctttacttgc caatatcgct
    10621 ttgaacccaa ttgcatataa tatagatgag aagactgaaa aagaaattta tgtaaaggaa
    10681 atgatgttgg aaggtataat aagcatacaa tctggggaga gcagtatagt agttgaggaa
    10741 cgtttagcca catacctttc aaacaatgag aagttagaaa ttatgaaatc caataaaaat
    10801 acagaaagag caatgagcaa tggcgcgtaa aaaaaataag aaaaaggatg agataaatcc
    10861 agatgcatgg ctggctactt atgcagatac tataacactg attcttacat ttttcgtatt
    10921 attgtatgca acatcagttg tagatatgga aaaatttaaa aacatagcag atgcgttaaa
    10981 aggtcagttt tctgggacaa gtatatttag tacgggttca agtacaagtg agaaatctcc
    11041 aatagacagt gttttagtgg ataaaaatga taaatcaaat ttagaacagg atttaaaaga
    11101 taaagttaat aattctaact tacaaaatag tgtaacagtc aaagatgata gtagagggat
    11161 tttactagag ttagatgatt caattttatt tgactctggg atttctgagt taaaagtaga
    11221 tagtaaagct actcttaata aagtatatga catgataaag atgatgaaaa ataagatagt
    11281 tatagaaggc catactgata atgtacctgt aaaatcaagt acacatgaat ccaattggga
    11341 tttatcttct agtagagcag taagtgttgt aagatacttt gtagaagaaa agggcatgga
    11401 ccctagacta ttttcggcaa caggatgtgg agagtatcaa cctttggttt caaatgaaac
    11461 agcaaaggga agggctgaga atagaagagt taatatactt ataactattg actaggagga
    11521 agaggtttgt tcaaaaataa aaaaattatt ataattattt tgatactgat tattttctca
    11581 gcgggagcag gtttttttat attaggagaa ggtggaaata aacaagttga caagattgca
    11641 ggtttgttta ttccaacagg aaaaggtaaa gatgaagtaa agaccgaaaa tcttgactta
    11701 gaagagatgg tattaaagct agctgatgaa ggtgtaagat atttaaaagt atcaataact
    11761 gtatcttatg attcttcata taaagatata gaaaaaaata cgcctttaat tagggataat
    11821 attataaatt gttttatggc aaaaagggca gatgatttta ctgctgataa tttaaagaac
    11881 ataaaagaag aaattaaaaa tacattaaat atacaactag gtgaaaatgt aattcaaaat
    11941 atatatttca caaatatagt agtgcaataa aaggatggta agacatgata ctttatttac
    12001 taaaaatatt aatatgtatc ccaataattt taatattgat agtcttatct ttaaaaatta
    12061 gccaaggaac tttattaaaa aaatataatg ataaatatgt aaaaatacta gatattgtaa
    12121 atataagtaa aaacaatagc ataataattt taaaaatagg tgaagaagga tgcattatgg
    12181 catcttcttc ttctagcatg agtaaaataa aagatttgac caaagaagaa atattaaaaa
    12241 tagaaaacga cattaaagaa agaaatttta aaattcaaga ttttaaaatg gaaagaatta
    12301 aaaagaaata tgatttcagt agatatattg gaaaatttac aaaaaaggaa gataagtaaa
    12361 tggaactatt atcaaaatta cctttggctg atgtaccata tacaagttca atgcaattgt
    12421 ttttatttct aactgcatta atgtttttac catttgtaat gttcatgatg acaagctttg
    12481 tcagaatagt tataagttta tcatttttga aatcagcatt aggagcacag caagcaattc
    12541 caagtcaaat tttggtagga cttgcaatag tattgacaat atttataatg agaccagtat
    12601 taaatgagat aaacgaaaag gcattacaac catatatgaa agaagaagtt actatggaag
    12661 aagctatgaa ggaagcagaa ggacctatta aagagttttt attaacacaa accagacaga
    12721 cagatttaga tttatttgta gaacaggctg gtttaaaaga aaagaaatta actcgtgaaa
    12781 atataccact atctgttgta gttccagcat ttgctataag cgaactaaaa actgcttttc
    12841 aaataggttt tttaatatat ataccatttt tgataataga ccttgttgtg gcaagtgttt
    12901 tgatgtctat gggtatgttt atgttgcctc cagtcatgat atcattacca tttaagttat
    12961 tattatttgt aatggtggat ggatggaact tgatagtaaa aacattgata ttgggatttg
    13021 gataggagaa aaatagtatg agtgaattta tgataatgag tgttcttaaa aatggcatgt
    13081 ttacaggagc tatgattgca gctccagtac tcattgtttc tcttgtagtt gggcttttaa
    13141 taagtgtgtt tcaagctaca actcaaatcc aggagcaaac tttgactttt gtaccaaaac
    13201 taatagctat tcttttagta gtagctattg gtggaagctg gatgttacat actcttatta
    13261 gctttacttc tgagatattc aatatgatat ctcttataac taaataagta ggatacatat
    13321 gatttttgta tttattagaa tagtagcatt ctttggaaca ctaagaatac tatttccagc
    13381 tggtacacct gctttattta agtcactctt tgcaatcatt atatcaatat tgatttcaag
    13441 tactatgcaa attgaatatg caacaaatat agacaacatt gtattgttta ccttatatgg
    13501 tataaatgag accataactg ggattatgct tggatatata accaacctat gtttttatag
    13561 tatcagaatg gcgggttcta tgatggacca acagatggga ttatcaatga ttaatatgtt
    13621 tgacccaaat tcaatgactc agacaacttt aattgataat cttatgaact ggacagcact
    13681 tatgatattt tttagcatgg atggtcatca tgttttaatt agaggtataa ggtatagttt
    13741 tgagttaatt ccaataggaa aaccatttgt agataataac atagattata taataaacat
    13801 atttgtgcag tgctttttaa ctggattcaa aatagcaata cctattgtac tttgtctgct
    13861 tatggcagat tttattttag ggcttatatc aagaagtgta cctcaattaa atgtaatgat
    13921 agttggtatg ccattaaaaa tattggttgg tattgcatta tttatcatat caataccttt
    13981 aattgtcaat caaataagtc atttattaag tcaaatacca aagatgtatg agggaacttt
    14041 tgcactagct cctatgtttt ttatgggttc aacagataaa acagaagaag ctactccaaa
    14101 gaaaaaggga gaacaaagaa agaaaggtaa tatagcaaag agtagagaat tacccgttgc
    14161 aatgactcta cttgcattca ccttattagt acctacatta ttttcatatg tagtagatac
    14221 acttaaatct tcgcttaatt attttttgag tctagatttt tatatgaaca taaattattc
    14281 aaacctagag aaattagtta tagcaggatt aatggatttt tttaagatat ttttaccaat
    14341 agctattccc tttttagttt taggcataat agcaaaccta tttcaggtgg gaatactgtt
    14401 tactggtgaa actttaaaac caaatttatc aaaattaaat ccagttagtg gattcaaaaa
    14461 catgttttct atgagaagtt tgtcaacctt aataaaggat atagcaatta tatctatttt
    14521 ggcttatata ggatatacat tcttccaaga taattattta gatatattaa agctaggtaa
    14581 catataccta ccaactttaa tgtacacagt taaagattta gtatattcaa tattgagtaa
    14641 gatttgtgta gctatgatag ctatagcagt tgcagattat gtttatcaaa gatacagtca
    14701 taaaaagcaa ctgagaatga caaaacaaga agttaaagat gaatataaaa actcagaagg
    14761 agacccagaa gtaaaagcta agataaaaca aaaacaaaga caaatttcgt ctcaaaggac
    14821 aatgcaagca gtaccaagtg ctactgtaat agtaaccaat ccaacccact tatctatagc
    14881 agtgagatat gaaaaaggga aagaccaagc tccagtagtg gtggcaaaag gtgcagatta
    14941 tttagctttt aaaataaggg aaattgcaaa gggaaatgac atccctataa tagaaaacaa
    15001 acctattgct aggttgttat ataagcaagt agaaatagac caagaaattc cagaggacat
    15061 gtatcaagct tttgcagaga tattagtggc agtatataaa atcaaaaata gatacaaagt
    15121 ccccaaaagg taggataagt aatgaataag tttaatttaa aggaaaatat ggacgtaata
    15181 gtggcatttg gtgttgtagg gattgttctt atgataatag tgccattacc tacatttata
    15241 ttagatattt tactggcaat taacataagt ttatctgtgc taattttact aatgacatta
    15301 tttacaacaa atgtacttga tttatctata tttccaacag tgttacttgt gacaacacta
    15361 tttagattgg gattaaatct ttcatcaact agattgattt taacacaggg atatgcaggt
    15421 gaagttatag aggcatttgg aagttttgtt gtaggtggaa attacgtagt agggataatt
    15481 atattcttaa ttatattaat tgttcaattt gttgttataa ccaatggttc tggaagagta
    15541 tccgaagtaa cagctagatt tacattagat gctatgccag gtaagcagat gagtatagat
    15601 gcagatttaa atgcaggagt tatagatgac aaaactgcta gaaaaagaag aaaagagctt
    15661 caacaagaag cagattttta tggttctatg gatggagcaa ataagtttgt aaagggagat
    15721 gccatagcag gtcttatagt aacggcaata aacataatag gtggaattgt aataggagct
    15781 gtaatgctga atatgactct tatgggtgca gcacagacat atgtaagact tactgtagga
    15841 gatggtcttg taagtcagat acctgctcta ttaatatcta catcagcagg tataatcgta
    15901 acaagagctt ctactgatga aaactttggt ggattagtag gtaaacagtt aacagcaata
    15961 ccaaaagcag taatgatgac tggctgtgtt ctaatcgtac ttggtatgat gcctggatta
    16021 cctaaaccag catttttttc actaggtata ggtgctatag cagcaggtta ctttttagga
    16081 agagaaaaga aagatacttt agaagacttg gagtttgaaa aacctgatat ggtaaatcca
    16141 gataaaaatg aagtagaaga tgtaacaaac ttaataaatg tagaatcaat agaagtagaa
    16201 attggatatg gtttaatatc tttagctgat gaatcagcag gaggagattt acttcagaga
    16261 atagcatcaa taagaagaca atgtgcaata gacatgggta tagtcgtaca acctataaga
    16321 ataagagata atttacagtt aaatccaaac caatacaata taaaaataaa agggaatgta
    16381 gttggtgggt atgagataat gcctaatatg gttttatgta tggatcctat gaatcaagga
    16441 tttagtatat ttggaataaa gacaattgag cctacttttg gaatagaggc actttggata
    16501 gaaagtgaca aggtagaaga tgcagagtta aagggttata cagttgtaga ttcatccaca
    16561 atacttataa ctcacttact agatataata aaatcaaatg cacacgaatt actgggaaga
    16621 caagaagtta agactattat agatgcagca agagaaaatt atagtgctgt agtagatgaa
    16681 ttgataccag atttgatgac acttggtgaa attcaaaaag tacttaaaaa tctgttaaga
    16741 gaaaaagtaa atataaagga tagagttaca atacttgaaa cgcttgcaga ccagtctaga
    16801 aatacaaaag atatagagct tcttactgaa tatgtaagaa tagccttggc tagaagtatt
    16861 tgtaataatt tagttgatga tgataagaca attgtagtgg ctacattgtc tccagaaact
    16921 gaagagttga tatctaataa tttacagagg tctgtaaatg gaacttatcc agcagttgac
    16981 ccagaaaata caaataaaat atttgaaagt atacaagaag taatgagcaa tgtatatttc
    17041 aatcataata taccagtaat agtagtatca ccaaagataa gagctccatt tagaaaatta
    17101 gttgaaattg tatatcctaa tttgactgta ttatcattaa atgaaatacc taacgatatt
    17161 aaaatcaaag cagaaggtgt ggtgagcata tatgatgacg aaaaagtata cagctaacac
    17221 aattcaggat gctatgaatc tagccaaaat ggaattgggt gacaatatta cattaataga
    17281 taaaaaagaa gtaagaaagt ctggaataca aggtatcttt tcaaagaaag atattgaact
    17341 aactattgga tgggaaaaga gagaaaacat agaacaaaaa gatttaaaac gagagataga
    17401 acaattaaag ttaattatta acaatatggg gtttgataat aagaatgata atgatataga
    17461 taaaatatgt aaaaatctac taagtttaga attgaatgaa gaaattgtag aatctataag
    17521 aattgatttg caagaaatga agttcaatgg aatcgataca agtaaaaatt tagtagaaat
    17581 tttaaagaaa aagattaaaa tagaaaatca agtaataaat ggtaaaattg ctctagtcgg
    17641 accaccagga gtaggtaaaa cgactacaat agcaaagtta gccgcaaaat tagtttttga
    17701 agaaaataaa aaagtaggag taataactat agatacatac aggattggtg ctgtagaaca
    17761 gttgaagata tatactgata taatgaatat tccctttaaa ggggtaataa gtcctgatga
    17821 aatggaattg gcacttgacg agatgaaaga ttgtgatgtt gtattaatag atacaacagg
    17881 tagaggctat aaaaactcta tgcaaatact agaaatcaaa aatcttatag ataaagctga
    17941 aactgataat atacatttag ttttgaattg tacaacaaga gaaagtgata caaaagcaat
    18001 tatagattca tatagaaatg tgaattttaa aagcttaata attacaaagc tagatgaaac
    18061 aataacatat ggatcaatat ttaacataat gaattatgca caaaaaccaa tatcatatat
    18121 tacaacagga caaaatgttc ctgatgatat tataaaacct aatgaagata agattataag
    18181 gttactatta ggagttgaaa gtatatgata gaccaagctc aaattttgag aaaaatggca
    18241 atggaaaaaa gaggacttga tgagtttata gtagagaatg agaatgtacc taagataatt
    18301 acaatagctt ctggtaaagg cggtgttgga aagagcaatc tcgctacaaa tttgtctata
    18361 tgcctgacca aacttaataa aaaagttctt atattagatg cagacatagg aatgtctaat
    18421 atagatataa taatgggggt aaatgtaaaa gggactataa ttgatgtaat taatggcgaa
    18481 aaaaatatag aagatataat ttcacagact aagtatggtg taaatataat atctggaggt
    18541 tctgccctta atcatataga agactttaca gaagctcaaa gaaataagtt tatacatagc
    18601 atagaacaga ttcacgatgt ggattttata ataattgata caggagctgg tatgagcaaa
    18661 agcttattgt catttatata ctgtagtact gaatttttct taataacaac tccagaaccg
    18721 acatcattga ctgacgcata tagtttgtta aaagctataa gtaattttgg cataaagaga
    18781 agtgcaaata taataatcaa tagggtaatt gatttagaag aagcaaaatc tacatacaaa
    18841 agaataaaaa tggttgtgga caaatttcta aaccttaatc tagaattgta tggatatata
    18901 atagatgaca aaaaagtaag tttatgtgta aaaaaacaga taccattcat tttagaatat
    18961 cctacaagta ttgcatcaaa atgtattata gccatagcaa agagaatggt aagtcagtca
    19021 agagaagata ttgttgataa taaaagtttt ctaagaaaga tgtttagtat atttaaaagc
    19081 taaggaggcg tagttaatga atagagaaga attaataaaa gagaatatgc ctcttgtaaa
    19141 gagtatagca agaaaatttt ttataccagg gaaaggtttt gaatatcaag acttagtaaa
    19201 tagtggagtc attggattga ttgatgcaat aaataaattt gatgctgaaa aaggggcaaa
    19261 gttttctagt tactcttata taaaaataaa atctgctata ttagatgaaa ttagaaatca
    19321 aagtccaatt tctaaacata atctaactaa agtaaataag tataatagag tggtagaaaa
    19381 gttgcaatct aaattattaa gagagccaac ttcatatgaa attgcaaaag aattaaaggt
    19441 ttcagaaaaa gaacttcatg atatcgaaag taatatagat atgttaaata ttgtatcatt
    19501 aaactatgta atctttgaag atacaaatga aactgtacaa gatgtaataa gtgatagaga
    19561 agaggaagct ccagaaaata taatagaaga agaagaaaag ttagagattc tatccaaagc
    19621 aattagtaat ttaaatgaaa gagaaaaatt aatactttca ctgtactatt atgaagattt
    19681 aaatctaaag gagataggga aagtgctagg tgtttcagaa tcaagagttt cacaactcca
    19741 cagaaaatca ataagaaact taagaaacaa gataaaagaa cttaaatatt ctatatagat
    19801 ggtgattgat aagatgatac aaattatatt aatagtagtg tctattttaa taatagtaac
    19861 tatagttata aatttacata aggaatcaaa aaataaagat aacagatact ttgataaaat
    19921 atttgagcaa gaatatgaag caaatgatag attaataatt gcagaaaaaa gaagattctt
    19981 tttagacaaa gtacttgcag aaaaaagaat taatagtcat ataaaagttt atgaaaatta
    20041 taacctaata aaggctaatg aagaaagaaa ggaaaacata tttatagaat caaaaaagga
    20101 agtaagacaa gaaaatgagt tatctcttaa agtgaaacat cttataagtt caggttatga
    20161 tgatgtggaa atatgtaaga tgctagatat tggaaagggg gaactgttat taataaggag
    20221 ttgttacaaa atataaggat attattatgt gataaaaaag tattcttagg aataggtatt
    20281 ggtatattaa tatcaacttt atgtataatg ctttttagta ataatgatat gagtaaggca
    20341 gagatagaaa aaaaggccag agcgttaggc atgaaatata caagtgagat gaaagtttta
    20401 gaatagatga tattgtttag acatttacaa aaaaggaggt cataatgtga taagaggatt
    20461 atatacagca gtttctgcta tgataactaa tcaacaaaag caaaatgttg ttgtcaataa
    20521 cctagctaat atggatacaa atggatataa aagtaaacaa cttttgacta aaagttttga
    20581 tgaattaaag ttggagggtt acaataacta cgcaaatgga actaaacaaa agcagattat
    20641 aggcgatata agtccaggta tttctatgga tgaaactatc acaaatttta atcaaggacc
    20701 tataaaaact actgataata aaatggatgt agctattcaa ggtaagggat tttttctagt
    20761 tagtgatgca gctggaaatc aattttacac tagaaatggg aacttcagaa cagacaatca
    20821 aggtgacctt ataacaagtg aagggtatta tgtacttggg acgaatactg ctactggtgc
    20881 tgtaggtcat ataaatgtag gaaatcaaaa atttgaagtg gcaaaagata atacaatatc
    20941 actagacgga aatgctatgt ataaatttaa tattgtagat tttaataact acaataactt
    21001 aaaaaaagaa ggagataact tatattctgg aggaggagct atgaaagtaa ataatgcact
    21061 tacaatacaa agtgcgatag agtcctctaa tgtagatatg gtatctgaaa ttaataatat
    21121 gatgacaata tcaagagaat atgaggcaaa tcaaaagata attcaggcta tggattctaa
    21181 attagcaaaa atagcaagtg agattggttc agtaagatag gtaataggag gtgaattttt
    21241 atgtacacaa tgttttatac tagtaaaaca gctttatcag caaatcaaag taaactagat
    21301 ataatatcta acaatatagc aaattctgat tcaacaggat ataaaaaaat aaatatggaa
    21361 tattctgatt tagtggatga agtactaaat agaccatcat atcctacaaa tggcaatgat
    21421 atatcgactg gaacaggagc taaggcaaca gctcccatga gaaattatga acaaggtgca
    21481 ttggctccta cagatagtaa gagtaatttg gcaatagatg gagaaggatt ctttagagtg
    21541 ataagaaaag atggtacata tgcatataca agaaatggtg gatttaatgt agatgcttta
    21601 ggtaagattg tagatgataa tggaaatata ttagatgtcc aatttgatgc aggatataac
    21661 tataataata caaatataga tagtacaaac ctgacagttt caagaaatgg agagctattc
    21721 gtagataata aaaaaatagg aaaaatcaac ttatatcaac ctataggaac tcaaaatttt
    21781 atatctgaag gagatagttt attcgtcgca actgatgcag cacagattaa acaagttgag
    21841 aaagtcgata tagttcaagg ctatagagag aggtcaaatg tttctttaca agaagaattt
    21901 gttgacttaa tagccactca aagagcattt caaatgaact caaagggaat aaagacagca
    21961 gatgaaatgt ggcaaatagc gaataattta agagctaaat aattatgtaa ggtgaggaat
    22021 attcatgaat gatatgaaag agtacgattt tggcaaacct cagagttatt caagagaaaa
    22081 tttaacttgt ttaaattttt tattagctga attttgtaaa aaatataaaa actatataat
    22141 ttatgaactt aagtgtaatt ctagtatggt tgttgatagt atagaccaag taaattatca
    22201 gactttttta gatagagtaa cttcttcatg cgtagtagta caaaatgcaa tggaacctgt
    22261 tatgaaaaac ttttctttta gaatagatag aagtgtagct gatatgtgga ttgatataac
    22321 ttctggagga actggagttg taaaggataa tgatagagaa cttacagaac ttgataaaaa
    22381 agtgctttta caccttatgg atgatttagt aagaaatatg cacttatttg agggttttga
    22441 aaatatacaa gtattggata cacatacaca tattaatttg ccacaattat gttctccaac
    22501 tgcaccagta tgcgttgtag atttaaaagt gctaaatgat aatgagtata ttggaagtgt
    22561 ttctctttgc tttccataca atggtttaga ggctctgtta gaaagtatat ctgttataga
    22621 attttttgat aatgatgtag gagatgattc agaggaattt acccaaaaaa tctataatag
    22681 tgtatctaat atagaattat cagttattgc agagatagga aaagtatcaa ttaatgttga
    22741 ggatttactc aaactagaag ttggagatgt aatagtaaca aataaaaaaa taaatgatta
    22801 tatagatata tttgtagaaa attcaaaatc atacacagca acaccaggat ttatatcaag
    22861 taaaaaaggt gtaaaaataa atgatgctgt tggaaaagag gtgtagttat ggacaatagt
    22921 aatcttattt cagatgaaga agcaaagatg ttattaaatg ataaagttga aagtgaagtt
    22981 gatggagatg atgagttagc cttagatgtc tctgaagtta atcgaaataa tatacaaagg
    23041 atacttgacc ttaagttaga attatctgta gttataggaa gaactaagat gactctaaga
    23101 gatattttaa atttacataa aggttctctt attgaactgg atactttggc agaccaagat
    23161 gtggaaatac taatagataa taaggttttg gcttatggaa aagtagtggt ggtagattta
    23221 aattttggtg taaagataac aagtatagta agtgaagaag acatggtaaa atctcttgtt
    23281 tagagataac atcaattaat acttaaggca tgaggtggta cttattttaa acttaaatga
    23341 tataaaaaaa gatatactaa aactcaataa aaaagactca tgttctctat ataatgaatc
    23401 tattaataat ataaataata attgtatatc aactggatta aaatttttaa aacaagcaag
    23461 agatttaaac ccagatgatg ttgatatatt aaatctcaca ggtcttgtaa acttactaaa
    23521 atgtaatttt gatgatgctg tagaaagttt ctataagagt tcactttgtg aaaaaactgc
    23581 tctgtgtgaa aagtatataa acatgcttac atctgaggag tttttaattt tctttgaaaa
    23641 gtacaatcaa cttataaggt taataaataa aggaatgtat aaagaagcta tacaagggtt
    23701 taaacttata actgaccaat actgtgattt aatagaacct tatgagttgc ttgccttact
    23761 gtatgacaag gaagaagaat atataaaatt tgatgaatgt ttagaagtat tgaagattat
    23821 tgataaggaa aattatttat taaatcaaga aaataacaca ttatatatgt ataatagtgc
    23881 taatatacaa actaaaagta atataaaagt agatgattat aagaatgagt tcttacataa
    23941 aagtagtagt aaccataaaa ttaatgatta taatgatgta actaaagaaa agagttcaag
    24001 caaaaaccag aaaagttcag attctaataa gaaaaaaagt attaaaaaaa ataagtacct
    24061 atatatagcc ataatattag gaatattatt agtaggacaa agtatatata gttcttataa
    24121 gatgaataat ttaactgata aaatatctaa aaataatgaa actaaaaatg aagagtcaat
    24181 agattctaac ttgactaaga aagaaaataa tatagaagaa aatgaagata tgagtaaggc
    24241 ttctaatata aataagagta ataaaaacat taataaagag gatgatgtaa aatctaaaaa
    24301 aaatactgta gaaaatgaag atgataaaga ttctgactta tttacagagg atgaattgat
    24361 gtctaaggcg aatactttaa aatcagaaaa gaaaactaaa tcttctatac aactatataa
    24421 gaaagttgca gatataggtg aaaataaagg gaatacatct gaagcaactt atcaagttgc
    24481 tattctcagt gaaaagttaa aagactataa aactgctgaa gaatattata aaatgtatgt
    24541 agaaaattac tcagaaaaag atgcttattt tgatgaatct tattataatt taggtatgat
    24601 gtactataac aatggtgatt taaaaaattc taaattgaca ttaaagaagt tggttaataa
    24661 ggtaccaaat agtatgtata ataattcaaa agtaaaagaa attttaaaag aagaatagtt
    24721 aaattagata agaaaaataa attattttat tttaaaataa tatgattata atatattgac
    24781 ataccatttt aaaaaattta aaatattaat tataaaatga ggagggataa aaaatggaat
    24841 ttgaaaaagg gagtatttca attcatacag aaaatatttt tcctataatt aaaaaatggt
    24901 tatactctga caaagacata tttataagag agcttataag taatggatgt gatgcagtaa
    24961 gtaaacataa aagacttgta tcactaggag aaatatcaga aaataagtca tcagattata
    25021 aaattacagt atctgtaaat aaaggagaag gtacacttaa atttattgac aatggaattg
    25081 gtatgactga agaagagata aaaaaataca taaatcaagt tgctttttca ggtgctgaag
    25141 atttctttaa taaatataag gataaaatgg aagagtcaaa tgatataata ggtcattttg
    25201 gactaggatt ttattctgca tttatggtgt ctaaaaaggt acaaatagat acactttcat
    25261 atacagaagg agcgactcct gtaagatgga tatctgaagg tggtacagaa tacgaaatat
    25321 ctgaatcaga tgctagaaat gatagaggaa ctactataac actatttatt gatgatgata
    25381 gtaaagaatt tttagatgaa tttactgtaa gaggaataat aaataaatac tgttctttct
    25441 taccagtaga aatatattta gaagatgttg aaagattaga aagagaagct aaagaagcag
    25501 aagaaaaagc taaaaaacaa aaggaagatg gaaaagaaga gattgtagat gcaaaagtaa
    25561 ttgagccttt aaacgacact aatcctctat ggttaaaatc tccaaaagac tgtactgatg
    25621 aggagtataa agagttttat agaaaggtat tcaatgtatt tgatgaacct ttattttgga
    25681 tacatttaaa tgtagattat cctttcaatt taaagggtat actttacttc cctaaattaa
    25741 aaaatgagtt tgaactgact gaaggaaaag taaaattata caacaatcaa gtgtttgtgg
    25801 ctgacaatat aaaagaagtt ataccagaat ttttactttt attaaaagga gttatagact
    25861 gcccagactt accattaaat gtatcaagaa gtttcttaca aaatgacagg gatgtaagca
    25921 aaatttctaa acatataatt aaaaaagtag ctgataagtt gaaatctctt tgtaaaaatg
    25981 aaagagaaga atacaataag ttttgggatg atatacaaat atttattaaa tatggttgct
    26041 taaaagatga aagtttctat gagaaggtaa aagaatgtat attattcaaa actattgatg
    26101 atgagtatat aacattacaa gattatttag aaaaatgtaa ggacaaacat gaaaataagg
    26161 tattttatgt aagtgataag gaacagcaat ctcaatacat taaactattt aaagaatacg
    26221 acttaagtgc tgttgtttta aactcatcaa tagatactca ttttatttca tttatggaat
    26281 ataaagagaa tggagttaag tttaatagaa tagatgcaga cctttctgat gtattaaaag
    26341 ataaaaatga aaataaagat agtgaagaaa acaaagaaga gatagctaag atagaaggtt
    26401 tgtttaaaga agctgtcgga gaaagagtga agaattattc tgtagaagga ttgaaaaatg
    26461 aagacactcc agctatggta ttagtatcag aacaatctat tagaatggca gaaatgcagt
    26521 caagatttgc tggaatggat ttaggaatga attttgagga agaaaaaact cttgtaataa
    26581 atgagaacag tccaataatc aaaaaattgg tttctcttaa agatgatgag gaaaagaaag
    26641 ataagattac gcttatttgt aaccagatag cagatttagc tttactttct aataaagaat
    26701 taaagccaga tgagttagat tcatttgtac aaagaagtaa taagcttatg agtcttttga
    26761 tagaattgta agattgaatt tgaatacatt gaagcaaatt tataaaaagt attcaatatc
    26821 agtgatgaga aagaagtgtc tcaaaatgaa cttttcagtt catgaggcgc tttttttatg
    26881 tgaaattaaa tttattttaa tcatttcaac aatgaaaaat atatttatct ctattttgag
    26941 acagaccgtt ctttattttt ttatctttgg ttaattttta aatatttaag taggttctaa
    27001 ta
//
LOCUS       DAELLV010000028        25159 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.028, whole genome shotgun sequence.
ACCESSION   DAELLV010000028 DAELLV010000000
VERSION     DAELLV010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25159
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.028"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            308..2164
                     /locus_tag="KPD58_001020"
     CDS             308..2164
                     /locus_tag="KPD58_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBG0264803.1"
                     /translation="MDTHNKYVNFIKNIPVPFLYCRIVKRQEDIEYRVEYISKGMGKV
                     LQLEEGICDKNILDVLPVFKSKKYFKELFSNEVDCIKRYIPTLKNWINIKKQIIGDSY
                     IILYFGKIVFDYRQIIDSFDKKEKVAYIKDEEGIYIDCSENLIPILNNNIKTTKDIFG
                     KNDIEVWGENTGKLFRDDYREGVSSKKRFLQNLFEYEETFFMVEKYFLYDEDELLGTI
                     GIVDNIIYSGYSNRNYNSKDLMKMIEHSIPENMFYKDVYGNYIGFNSGFLNLACMNKE
                     ELLGKNSYKISEEEALIDKIFESDKGVVENKKVVTFELNISMNDENKCIEITKRPFFD
                     SYGSVIGIIGTARDISRRKRLEEEMDKTRMEFFANLSHELRTPINLISSSLQVIEKKE
                     ADLIESNDTLKRNLGIIKQNGNRILRLVNNVIDFTKMQSGYLDFKPEESDIIAFIEEI
                     CMSVADFASQNNIQLTFDTEIEEFSMLFDSEKLERIILNLLSNGIKYNKKDGKINIFL
                     YVKDNVFNMKISDSGIGIPKEKIDKIFNRFEQIDNELSYRVKGSGIGLSLVKSLVELH
                     EGSISLKSQLGIGSEFIVSLPVRSKNNIEKYNHKREISNELSKKLEIEFSDL"
     misc_binding    2247..2487
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    2553..2788
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2873..4090
                     /locus_tag="KPD58_001021"
     CDS             2873..4090
                     /locus_tag="KPD58_001021"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HBG0264804.1"
                     /translation="MENKVKIGVLGYGVVGSGLIDIIDNNKEKRNIEIVGILVNNLEK
                     HKDKKYSNIITNNIDDIFNKDIDILVEVMGGLEPSLSYIKKALNNKIHVVTANKDLLA
                     ECGDELAKLASENKVSIKFEASVAGGIPVLKPIIESLEGNNIDSINAILNGTTNFILS
                     KMYDENLSYDMALRQAQELGFAEANPESDVLGYDAARKLSILSTLAYDNRVYWKDVYL
                     EGITDIDEKDIEYAKKLNCKIKLIGQSKYENDKVSAFVRPVLVEKDNILARIDNEFNA
                     VIVNGDSVGEVSFVGKGAGSLATGSAVYSDVIDIIDNRVSSIDSFTKDKIQVNKIVRE
                     KCGALLRFKKCNKDEILNIVENCLVKFDILNDDDELAIMVYADSEYEINNSLCLIKDK
                     GYCEKMNKMLKIS"
     gene            4207..4689
                     /gene="cdeM"
                     /locus_tag="KPD58_001022"
     CDS             4207..4689
                     /gene="cdeM"
                     /locus_tag="KPD58_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeM"
                     /protein_id="HBG0264805.1"
                     /translation="MENKKCYSEDWYERGESTAKWFQNDREEYEREAYDEDRERRGSN
                     CGCSDSGENRPRNCERFRREAEIREREAREAFCESSEKKKEALAYECEARKLWEEAEK
                     YWDEYSKYNYKGIEYLAEAARLFDEGMECEARRNGNNGGNNNNCCHKCHKCNCNCCRK
                     "
     gene            4954..6219
                     /gene="hisD"
                     /locus_tag="KPD58_001023"
     CDS             4954..6219
                     /gene="hisD"
                     /locus_tag="KPD58_001023"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HBG0264806.1"
                     /translation="MKSIKTSSMKEKVDEKILREQVSSIIEDIRNNKDVALKKYNEKF
                     DRNTRDEFRITKEEIKEAYKHVDDEFINNLKIAAKNIREFAKAQKLSFENPFEKEIYP
                     GVILGQTNIPIESCLAYVPGGQYPLFSTALMLIIPAKVAGVKRIVACSPTMKNTEKIN
                     PKTLVAMDIAGADEIYATGGVQAIAAFTYGTEKINPVDIIVGPGNKFVTEAKRQCYGQ
                     VGIDFVAGPSEVLIIADETSNPVYIAADLLAQCEHDLNARGILLTNSLEVAQKVEKNI
                     ESMLKDLPTKDIAYSSWENNGEIILVDDMEEAIKISNFYAPEHLEVAVNEYNDICDRL
                     TNYGSLFIGNLSAEVFGDYVSGTNHTLPTLKASRYTGGVWVGTFIKTCTKQIFNEKAI
                     QSLAPVAEKLAKEEGLYAHAKAAEVRFKK"
     gene            complement(6422..7174)
                     /locus_tag="KPD58_001024"
     CDS             complement(6422..7174)
                     /locus_tag="KPD58_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0264807.1"
                     /translation="MSYFQYQGNSCFYKEYGQGKPIIFLHGNTGSSNMFKALVPLYAE
                     NFRCILIDFLGNGQSDRVSQFSPDIWHDEALQTIALIEHLNCGKVGLIGTSGGAWAAV
                     NTGLERPDLVEAIIADSFDGRTLNDNFIDNLITGREKSKQDIQARKFYEWCHGKDWEN
                     VVNLDTKALLQCANEKRPLFHKELCKLEMPILFTGSKEDEMCRHNLEEEYKQMATFIS
                     KASIYIFSQGGHPAILTNADEFAQLAKVFFKY"
     gene            complement(7292..7561)
                     /locus_tag="KPD58_001025"
     CDS             complement(7292..7561)
                     /locus_tag="KPD58_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264808.1"
                     /translation="MSYQNSIDLLPKELIEQVQEYIDGKVIYIPKKQEHKKHWGENTN
                     TKQVLASRNSQICINFQNGMSIKQLSEKYFLTEKSIQRILKQQNL"
     gene            8036..9130
                     /locus_tag="KPD58_001026"
     CDS             8036..9130
                     /locus_tag="KPD58_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBG0264809.1"
                     /translation="MKNIVGKPQTIKKVNEDLIKNIIKNEGPITKPEISSITELSLAT
                     VNKIVEQLALKNEVKVSGLSESTGGRRAQLFEINANLEHIIALYYYRNCCIGVVANLL
                     GEIIYQEEFNIRMDTYENVNQDTFFVIDTLIKKEKGNNIRAIGIGVPGVVKKGVVKNI
                     PTIKSWEGINLENLIKSKYNTKVFIENDTNITTIGIYQKQYKDKYKNMALMYLEDGIG
                     SGIVINGELYLGSTNFAGELSYLNIEFGKYNSNNLNLEDYIMSLRKRYLESKDNKFKK
                     EILSIISNFIRTLVCVLNPQAMIIQCSILTKSDMEFIEKDVSNNVGEENFPKLIKVDS
                     LREGSINGVISMCLKETDYQYSLSNKKGGY"
     gene            9132..10082
                     /locus_tag="KPD58_001027"
     CDS             9132..10082
                     /locus_tag="KPD58_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HBG0264810.1"
                     /translation="MKYLVSIDGGGTKTKFCVSDLDGNILKEHTTGSTNYKSVGIKKT
                     YENINNGFKKILKDLYIDYDDIEYTVFGISGCDSPNDYKIIMDEILKIGINKEKIYLA
                     NDAVLAFYAQADSPGLVIVAGTGSIILGIKEDGEIYRVGGWGYNFSDLGSGYDIGRKL
                     LKKVLLYCDECHEYSDLFSCVLDFFGANSFEQLAYMITDINDNVEIANLASLVIDCAE
                     QGDKLAIEILRESSTELSKLAQVILRKISNPMKMDKSEINIVLSGGTLNKTVYAQMLM
                     NNLKTENTDKNLKFISQENQPVYGGIRLAMALANRRVKHG"
     gene            10075..11259
                     /locus_tag="KPD58_001028"
     CDS             10075..11259
                     /locus_tag="KPD58_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264811.1"
                     /translation="MDRKKVIVQIFTGGFKAKEIKFNDIKCKLLELLDCIDIEKVIMG
                     WSVDEELYVETKKLLNKYNVELYLWLPCFSEIGLLEKSNLLIDYMGKEVKGYALQEDE
                     NFEFYCPNNKENIKNIKKIYSTYFSDIKFDGIFLDKIRYGSFSNKLNGVFNCFCKDCL
                     NIYKKNNIDKESLMREMNNVSKGVIDNEDTPFGITEYINGKYKFKNKIWEDFFETKNQ
                     NIYKALLEISSYFKSKGLKIGIDAFSPFISYFTGQDLEKLQEVADFIKPMMYRTTKAP
                     AGLPFEFERFIDESIHKNKEIGKRNFLDILGIKEYEGDVYPIEFVLEEIDFMTRNLNK
                     DIYVGVEINKKEIISPVTPKYISENISNINKTTVKGYVLSWDLLSAPKENIDEIIKWF
                     RN"
     gene            11272..12768
                     /locus_tag="KPD58_001029"
     CDS             11272..12768
                     /locus_tag="KPD58_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBG0264812.1"
                     /translation="MSKILEIQQIYKSFGKVDVLKGINFELEKGEVRALLGANGAGKS
                     TLIKILGGVHSQTKGKVILTGNEIMFKNPHHSKENGISIIYQELSLIPTLTVLENIFL
                     GREILKGSVFLNKKDMLEEYMKICEDFDFNIDPHIVVSNLSVANQQMVEIMKAVSCNS
                     EIIIMDEPTTSLTNNEKDGLFKIISRLKSKGKTIVYISHILEEVFKVCDSASIMKNGI
                     MIGTYDVEDLTKLKITQLMTGNDNHKLIEKKDYTFAKYEEPPVLEVKNISWSNKIKNV
                     SFKVYRGEVVGLAGLVGSRRTEIINLIFGIDNKDSGDIFINGKKVDITSPKVAIKNKI
                     GLIPEDRKNLGLILEQAIYTNSSSVQIDKFKKFLFIDRNKEIEFSKKGVKELGIKVSD
                     VKQEVKELSGGNQQKVVVSKWLEKDLDLIIYDEPTKGIDIAAKEDIFNTIKEFSSKGI
                     GVIFISSDLEEVIRVSDRILVTREGSIVDDLKNENIQVQDIMNSIFNV"
     gene            12791..13741
                     /locus_tag="KPD58_001030"
     CDS             12791..13741
                     /locus_tag="KPD58_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0264813.1"
                     /translation="MKKAFEKLNLRDYGVLLGFLALCIAISIATPSFLGKQNILNLLR
                     QSSIIGIISAGMTFVIISGNFDISVGAVAALAGAITMKFATGGTNLFASMFLGVAICA
                     VIGLINGIVVAKINVPSLIATMAMVTIVRGLLLMLTGGYPITENIPILDYIGNGYLFN
                     IPIPVVIFFIVVLASFIVLNKTKFGRYVYSVGGNQEASKLNGINVDSHKVKVFIINAV
                     LAGIAGIVLTGRLGTATAIAGEGYDMDAIASVVIGGTSVAGGSGSVLKTVIGVLLMSV
                     INNSFNLLGIDVYFQYIFKGLIILAAVGFDSYSKKKLASR"
     gene            13762..14724
                     /locus_tag="KPD58_001031"
     CDS             13762..14724
                     /locus_tag="KPD58_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBG0264814.1"
                     /translation="MKKLMKHLALLLSLVMIFGLVGCSNGGDKEKNGEKKIAVLLPGS
                     TGYFVATKQGIDAKSKELGVSVEYADAQWDASKQLSQAEDFMAKGVDMIALCGVDSAV
                     SERIVKAANDSDVPIVAFTNSIGSNPTGEFKGLVTYIGQNEEETGALTGKIAKNLLGE
                     TGGKAVLIEGVPGTTPQVLRKKGLEKELKDSNIEIVYNQTSNWEKEQALKVTEDLIQK
                     KTDFNVIICQDDNSATGAGQALKDAGLKDKVKVIGLGGSKDGLKAITDGLIDGTTYMS
                     AVEEGGLVIEKASKFLKGEKIEPVTQIKQVEVNKDNISEFKGEW"
     gene            15096..15728
                     /locus_tag="KPD58_001032"
     CDS             15096..15728
                     /locus_tag="KPD58_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG0264815.1"
                     /translation="MARRLFLERIGLIFLGSAILAFGVYNFYYLNNITEGGVLGILLL
                     LKNLFNIQPAIANVVIDSLLLLVGYKFFGKKFLIYSIVASITFSVLYDLFEAIGPLVP
                     QSQNMLLSTILAGVTVGTGVGIVVKAGCASGGDDALALVISKTTSLNIGQVYLATDVI
                     VLLLSLFYLSAFDIFYSLIAVTISGKVIDFIYYHGKSLDMDISNDIVPEC"
     gene            16139..16231
                     /gene="kdpF"
                     /locus_tag="KPD58_001033"
     CDS             16139..16231
                     /gene="kdpF"
                     /locus_tag="KPD58_001033"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HBG0264816.1"
                     /translation="MEGLPMWFLAVVIIMLIIYLVYALINPEKF"
     gene            16272..17954
                     /gene="kdpA"
                     /locus_tag="KPD58_001034"
     CDS             16272..17954
                     /gene="kdpA"
                     /locus_tag="KPD58_001034"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HBG0264817.1"
                     /translation="MFMVQIVIVLAIFMILVIPMGKYLYHIATNQKTFGDRLFDKVDN
                     FIYKVCSIDKKKEMNWKQYALALLFTNAVMVFIGYIILRTQSMHIFNPSGIKSMEQGL
                     SFNTIISFMTNTNLQHYSGESGLSYFSQMTVIIYMMFTSAATGYAAAMAFVRGLVGKK
                     KTLGNFYVDLIRITTRVLLPGALIIGLILVTQGVPQTFAGTETVTTIEGKLQDIARGP
                     VAALESIKHLGTNGGGFFGSNSSHPFENPTIISNIVEILSMMILPGACVVAFGHMIKN
                     KKQGWVVFGAMSIIFLIGLVVCFKAESAGNPILSQLGLNQSMGSMEGKEVRFGIAQSS
                     LFTTVTTSFTTGTVNNMHDTLTPLGGLVPLLNMMLNVVFGGKGVGLMNMLMYAIIAVF
                     LCGLMVGRTPEFLTKKIEGKEMKLIALLIILHPLLILMFSGLSVAIPAGLEGISNPGF
                     HGLSQVLYEFASSAANNGSGFEGLGDNTMFWNITTGIVMFFGRYVSIIVLLAISSLLA
                     SKKAVNESIGTLRTDNFTFTIVLVLVVLIVGALTFFPALALGPISEHLVLWH"
     gene            17979..20036
                     /gene="kdpB"
                     /locus_tag="KPD58_001035"
     CDS             17979..20036
                     /gene="kdpB"
                     /locus_tag="KPD58_001035"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HBG0264818.1"
                     /translation="METKKTKFITKDIFKTSIIEAFKKLNPKYMMKNPVMFVVEIGFI
                     VTLILTIVPSLFGDEGNNLRAYNGIVTIILFITVLFANFAEAVAEGRGKAQADTLKKT
                     RKDTVARVIDTLGNEKMISASELKLGDIVLVNAGEVIPNDGEVIEGIASVDESAITGE
                     SAPVMRESGGDFASVTGGTTVVSDWLKIRITTKPGESFLDKMIALVEGASRQKTPNEI
                     ALNTLLVGLTLIFMVVLISLYPMANYAGVKIPISTMIALLVCLIPTTIGGLLSAIGIA
                     GMDRVTRFNVIAMSGKAVEACGDVDTMILDKTGTITYGNRLASEFIAVGNADSKDLIN
                     YSVMCSLKDDTPEGKSIVDLGMKLGSTGKTKEAEEAEFVEFSAQTRMSGINLADGRAI
                     RKGAYDSIIKRVKESGGTVPEDLEENVNRVAKLGGTPLVVCVDNEIYGIIYLKDTVKS
                     GLVERFARLREIGIKTIMCTGDNPLTAATIAREAGVDGFIAECKPEDKIDAIKKEQLE
                     GKIVAMTGDGTNDAPALAQADVGLAMNSGTTSAKEAANMVDLDSDPTKILEVVEIGKQ
                     LLITRGALTTFSIANDVAKYFAIIPAIFTLAIPQMEILNVMKLSTSFSAILSALIFNA
                     IIIPCLIPLAMKGVKYRPMKSQAMLMRNMLIFGLGGVIVPFLGIKLIDLLITPLVMVL
                     NLG"
     gene            20055..20681
                     /locus_tag="KPD58_001036"
     CDS             20055..20681
                     /locus_tag="KPD58_001036"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HBG0264819.1"
                     /translation="MKTLKPALLVSIVLLVVCGLVYPLVLTGVSQVAFKDKANGSMIE
                     VNGVKVGSELIGQSFTDVRFFKGRVSSVNYNTYTKEDLVPDKDGNTSYGGVSSGSFNY
                     GATNPELHDRVQKDIEQFLKDNPTVKREDIPTDLLTASGSGLDPNISPASAKIQIPAI
                     AKASGISESKLQKIVDDNTSKKLFGVLGEDRVNVLKVNVEVAKILGLI"
     gene            20918..21826
                     /gene="cysK"
                     /locus_tag="KPD58_001037"
     CDS             20918..21826
                     /gene="cysK"
                     /locus_tag="KPD58_001037"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBG0264820.1"
                     /translation="MLYNNALELIGNTPVVRLNNLGCTNGCSNIYVKLEKVNPAGSIK
                     DRAVYEMLEGLEQRGELKKGDVLVEATSGNTGIALSMIGKLKGYEVVIVMPETMSVER
                     RDLMKAYGANLVLTDGKAGMAGSIEKANELLKENPNYKSLKQFENKDNPNAHYKTTAV
                     EIMNDVKDLDIFVCGVGTGGTLIGTARYLKEQNPNIRVIALEPKKSPAISENKTGPHK
                     IQGIGANFVPENYDSSVVDEVILVDDEDAFDTVKLLAAKEGILVGISSGANIFGAIEM
                     AKKYPDKKIVTVAPDGVDKYMSMGIF"
     gene            21853..22443
                     /gene="cysE"
                     /locus_tag="KPD58_001038"
     CDS             21853..22443
                     /gene="cysE"
                     /locus_tag="KPD58_001038"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBG0264821.1"
                     /translation="MFKKINKDIEYIMKNDPAARSKIEVFLLYPSVHAMIMHRMAHAL
                     YKKKKLFTARLISQISRFMTGIEIHPGAKMGEGILIDHGMGVVIGETAEVGNRVTIYQ
                     GATLGATGKDTGKRHPTVGDDVLIGAGTKILGPLNIGSNSKIGANSVVVKDVPNGATV
                     VGIPAKIVKIRNLEPVKKNKKEVSYEYDELDNVYYI"
     gene            22603..22791
                     /locus_tag="KPD58_001039"
     CDS             22603..22791
                     /locus_tag="KPD58_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBG0264822.1"
                     /translation="MKANVNQDTCIGCGLCPSICPEVFDMKDDGKSHVIVDEVPSDAE
                     ESAAEARESCPVDAIDVH"
     gene            complement(22845..23714)
                     /locus_tag="KPD58_001040"
     CDS             complement(22845..23714)
                     /locus_tag="KPD58_001040"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HBG0264823.1"
                     /translation="MKLNRNDSCWCGSTKKYKKCHMDMDNKIDSFAKLGHIVPTHDII
                     KNESQIEEIKKSAVINTKILDYISDKIEIGMSTEDINKLVHDFTLQNGAIPAPLDYNG
                     FPKSVCTSVNNEVCHGIPSKDIILKDGDIINVDVSTIFNGYYSDASRMFMIGNVNENA
                     KKLVQVTKECVQKGLEVAKPWGFLGDIGDAINSYAKKNGYSVVREIGGHGVGLSFHED
                     PFVSYVSKKGTKMLLVPGMIFTIEPMVNEGTHEIFVDEENNWTIYTKDGKLSAQWECM
                     VLVTETGVEILTE"
     gene            complement(23911..25155)
                     /locus_tag="KPD58_001041"
     CDS             complement(23911..25155)
                     /locus_tag="KPD58_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264824.1"
                     /translation="MENNLNTAKRNNTELLQNDSLKVNLQIGVDFTLLSKSLKAFYKK
                     EKDFYCIALAPCNVSSEKANRKISIYDMIKEINILIESITDKNELLSEELFTNNLSEF
                     YDESLDEIYIDLKQAYLYIKKNTDSLNIEDSIEYVFDVDISNDIKAKENPLINFNSLS
                     LVVWSGETKHILQQMNILDLEDIHSYSIENNTNITIYENNTLQYKFNEESENIKDSSI
                     DIIEQMLILPDDLNYDKDEVENMKNRLAKINIKYLQTLKEKDIKIKLINSNLTDEPEF
                     SDLKYQLPPCWVRSGKTWKDVPGIYRNNSIVAKIGYSNPSYANVHSSKNLELHETAHA
                     IDKNVLNKKSNSEEFMEVFAQERYKLYDPKQVAHAYISKFIEEFFAESFVHYYLDEDS
                     KNTLKENCPLTYDFLEKLELNY"
BASE COUNT         9712 a         2688 c         4526 g         8233 t
ORIGIN      
        1 atcaaaaaat atactaaata aaaattatag tcctaatgag ttaatttctg tattttcaaa
       61 taatgcagta tttagtcatg tgtttttata tgtgcctgta tttctaatta ccaatatatt
      121 agaataagct actgtaatta ggtttagaaa aaagcgacat aaataattat aaacaaataa
      181 taaattacat aaaattaaat ttattcacaa gtatatctta taaaatatgt tataattaat
      241 agaaaattgt catataatta attaagtagt attattataa ataacattga aaggtagtgg
      301 ttagaatatg gatacccata ataaatatgt aaattttata aaaaatatac ctgtaccttt
      361 tttatattgt agaattgtaa agagacaaga agatatagaa tatcgagtag aatatataag
      421 taagggtatg ggcaaagtat tacaattaga agaaggtatt tgtgataaaa atatattaga
      481 tgtattgcct gtatttaagt ctaaaaaata tttcaaagag ttgttctcaa atgaagtgga
      541 ttgtataaaa aggtacattc cgacacttaa aaactggata aatataaaaa aacagataat
      601 aggggattca tacataattt tgtattttgg taaaattgta tttgattata gacagataat
      661 cgattccttt gataaaaagg aaaaagtagc ttatattaaa gacgaagaag gcatatatat
      721 agattgtagt gaaaatttaa taccaatatt aaataacaac attaaaacaa caaaagatat
      781 ctttggtaaa aatgatatag aagtatgggg agagaataca ggaaaattat ttagagatga
      841 ttatagagaa ggagtatcta gtaaaaaaag atttttacaa aatctttttg aatatgaaga
      901 aacatttttt atggttgaaa aatatttttt gtatgatgaa gacgaacttt taggtactat
      961 agggatagta gacaatataa tatatagtgg ttacagtaat agaaattata actcaaaaga
     1021 tttaatgaag atgatagaac actcaattcc agagaatatg ttttataaag atgtgtatgg
     1081 aaattatatt ggatttaatt caggtttttt gaatttagct tgtatgaata aagaagaatt
     1141 attagggaag aatagttata aaatatcaga agaagaagct ttaatagata agatatttga
     1201 aagtgataag ggtgtagttg aaaataaaaa agtagttaca tttgagttaa atataagtat
     1261 gaatgatgaa aacaagtgta ttgagattac aaaaaggccg ttttttgata gctatggaag
     1321 tgttattgga ataattggaa cagcaaggga tataagtaga agaaaaagat tagaagagga
     1381 aatggataaa actaggatgg aattttttgc aaatttatcc catgaactta gaacgcctat
     1441 aaatttaata tcatcttcat tacaagtgat agaaaaaaaa gaagcagatt taatagaatc
     1501 aaatgatact ttaaaaagaa atctaggtat aataaaacaa aatggaaata gaatattgag
     1561 gttagtaaat aatgttatag actttacaaa aatgcaatca ggatatttag attttaaacc
     1621 agaagaatct gatataattg catttataga ggagatatgt atgtctgtag ctgattttgc
     1681 aagccaaaat aatatacaac ttacatttga tacagaaata gaagaatttt caatgttatt
     1741 tgattcagaa aaattggaaa gaataatatt aaatttactt tctaatggaa ttaagtacaa
     1801 taagaaagat ggaaaaataa atatattttt atatgtgaaa gataatgtat ttaatatgaa
     1861 aatatctgat agtggaatag gaataccaaa agaaaaaata gataaaatat ttaataggtt
     1921 tgaacagatt gacaatgaac tttcttacag agttaaagga agtggtattg gactttcatt
     1981 agtgaaatct ttggttgagt tgcatgaggg aagtatatct ttaaaaagtc aacttggtat
     2041 tggaagtgaa tttatagttt cattgcctgt tagaagtaaa aataatattg aaaaatataa
     2101 tcataaaaga gaaatcagca atgaactaag taaaaaatta gaaatagagt tttcggattt
     2161 ataagtttaa atataaaaaa taaaatttta aaaaagtatt gacaattgat ttagaagggt
     2221 ttaaaataaa ttacaacata ataaataaaa aggcgatgaa taggaagagt aaatctaatt
     2281 cgattaccag agagaagagt ccattggctg gaagactttt taaattagag ggattgaaaa
     2341 ctacctagga gctactttct gaagtcatct gcattagtag attttagtaa gaatagtcgt
     2401 tagctgcgtt aaagtgtaaa gtgagttttc atgacatatg aaaactaaat taggtggaac
     2461 cgcgggaata atactctcgt ccttatgtta taaggatgag agttttttgt tatttaaaaa
     2521 tataaacagt aatcataata attatagaaa atataaaagc gatgaatagg aagagtaaat
     2581 ttaattcgac tatcagagag gaaagttcac aggctgaaag actttctaag tttgagaaat
     2641 tgaaaactac ctaggagctg tggtctgaag ttactttaaa aagttttagt aagactaacc
     2701 gttaactgcg ttaaagtgta aagtggattt tatcaataaa tgataaaatc aaattgggtg
     2761 gaaccgcgag tatggactcg tccctttata tagggatgag tctattttta ttgcaataaa
     2821 agattgaaaa taaaaaaaaa tcggaaaatt ataaatttag gaggaaaata aaatggagaa
     2881 taaagtgaaa attggagtac taggatatgg agttgtagga agtggactta tagatataat
     2941 agataataat aaagaaaaaa gaaatataga aatagttgga attttagtta ataatttgga
     3001 aaaacataag gataaaaaat attctaatat aataactaat aatatagatg atatatttaa
     3061 taaagatata gatatacttg ttgaagtcat gggtggttta gaaccatcat tatcttatat
     3121 aaaaaaagca ttaaacaata aaattcatgt tgttacagca aataaagatt tgttagctga
     3181 atgtggagat gagcttgcaa aattagcaag tgaaaataaa gtaagtatta aatttgaagc
     3241 ttcagttgca ggaggtatac cagtattaaa accaataata gaatcactag aaggaaataa
     3301 tatagatagt ataaatgcaa ttttaaatgg aactactaat tttatattat ctaaaatgta
     3361 tgatgagaat ctatcttatg atatggcatt aagacaagca caagaattag gttttgctga
     3421 agcaaatcca gagtcagatg tattagggta tgatgctgct agaaaactat caatattatc
     3481 aactctagct tatgacaata gagtatattg gaaagatgtg tatttagaag gaattacaga
     3541 tatagatgaa aaagatattg aatatgcaaa aaaattaaat tgtaaaataa aattgattgg
     3601 tcaaagtaag tatgaaaatg ataaagtaag tgcttttgtt agacctgttt tagtagaaaa
     3661 agataatatc cttgctagaa tagacaatga gttcaatgct gtcatagtga atggagattc
     3721 agtaggagaa gtttcttttg taggtaaagg agctggaagt ttagcaacag gaagtgcggt
     3781 ttattcagat gttatagata taatagataa tagagtttca agtatagatt cttttactaa
     3841 agataaaata caagttaata aaatagttag agaaaagtgt ggagcacttc ttagatttaa
     3901 aaaatgtaat aaagatgaaa ttctaaatat agttgagaat tgtttagtaa aattcgatat
     3961 tttaaatgat gatgatgaac ttgctattat ggtttatgct gattctgaat atgaaataaa
     4021 caacagttta tgtctaataa aagataaagg atactgcgaa aaaatgaata aaatgttgaa
     4081 aataagttaa aaaagaaaaa atataagttt atatgtaaca tattttgacg attttaatac
     4141 tatatagtat gagatgaaac aaaacatctc ataaaaaaat taaaattaaa gggagagata
     4201 tgttatatgg aaaataaaaa atgttattca gaagattggt atgaaagagg agaatctaca
     4261 gctaaatggt tccaaaatga tagagaagaa tatgaaagag aagcatatga tgaagataga
     4321 gaaagaagag gttcaaactg tggatgttca gattcaggag aaaatagacc tagaaactgt
     4381 gaaagattta gaagagaagc tgagataaga gaaagagaag caagagaagc attttgtgaa
     4441 tcttcagaga aaaagaaaga ggcattagca tatgaatgtg aagctagaaa attatgggaa
     4501 gaagcagaaa aatactggga tgaatattca aaatacaact ataaaggaat cgaatattta
     4561 gcagaagctg ctagattatt tgatgaaggt atggaatgtg aagctagaag aaatggaaat
     4621 aatggaggaa acaataataa ttgttgccat aaatgccata aatgtaattg taactgctgt
     4681 agaaaataaa aaaattaatt gacaaatcaa tagttaaaat atatcatatt atgtataata
     4741 aaaagaaaat tcagaaaaaa ataatgatta gaagaaatat aagtatattt attagacaaa
     4801 tagtgctcag ttagtgaggt gagtacaata tgattaaact cccatcctct agcttttgat
     4861 agagggtggg agtttttatt tattgatata tcttaaatga ggataatatt gtaaaataaa
     4921 tgagtatgag attgtaaaat taggaggtaa aaaatgaaaa gtattaaaac atcaagtatg
     4981 aaagaaaagg tagatgaaaa gatattaaga gagcaagtaa gttctataat agaagatata
     5041 agaaataata aggatgttgc acttaaaaaa tataatgaga agtttgatag aaatacaaga
     5101 gatgaattta gaattactaa ggaagaaata aaggaagctt ataagcatgt agatgacgaa
     5161 tttataaata acttaaagat tgctgccaaa aatataagag aatttgctaa agctcaaaaa
     5221 ctaagttttg aaaacccttt tgaaaaagaa atttatccag gtgtaatctt aggtcaaact
     5281 aatattccaa tagagtcatg tcttgcatat gttccaggag gtcagtatcc attgttttca
     5341 acagctctta tgcttataat acctgcaaaa gtagcaggag taaaaagaat agtggcatgt
     5401 tcaccaacta tgaaaaatac tgaaaagata aatcctaaga cattggtagc tatggatata
     5461 gcaggtgctg atgaaatata tgcaactgga ggagttcaag ctatagcagc atttacatat
     5521 ggaacagaaa agattaatcc agttgacata attgtaggac caggaaataa gtttgtaact
     5581 gaagctaaaa gacaatgtta tggacaagta gggattgatt ttgtcgctgg gccaagtgaa
     5641 gttcttataa tagcggatga aacttcaaat ccagtttata ttgcagctga tttattagca
     5701 caatgtgagc atgatttaaa tgcaagagga atattattga caaatagctt ggaagtagca
     5761 caaaaagttg aaaaaaatat agaaagtatg ctaaaagatt tgccaacaaa ggacatagca
     5821 tatagttcat gggaaaataa tggagagata atattggttg atgatatgga agaagccatt
     5881 aaaataagta atttttatgc accagaacat ttagaagttg cagtaaatga gtataatgat
     5941 atttgtgata gattaactaa ctatggttct ttatttattg gaaatctttc tgctgaggtg
     6001 tttggtgact atgtttcagg taccaatcat acattaccaa cacttaaagc atcaagatat
     6061 actggtggtg tttgggttgg aacttttatc aaaacttgta caaagcagat atttaatgaa
     6121 aaagctatac aatcattagc acctgtagct gaaaagctgg caaaagaaga gggattatat
     6181 gcacatgcaa aggctgctga ggttaggttt aaaaagtagt aaaaaatttt attttggcag
     6241 tcgagactta gtattagact gagataagga aaacgctgag aacctgaata aatactatat
     6301 tttgtgggtc tcggcgcttc ttttatatct gtatataata gaataatact atctgtgtgt
     6361 ttaaatacta acttaatatt tcttttctaa ataatgttta tgaaatagtt aatattttta
     6421 gttaatactt gaaaaaaact tttgctaact gtgcaaattc atctgcattt gtcaatatag
     6481 cagggtgtcc tccctgacta aaaatgtaaa tacttgcttt tgaaataaaa gttgccattt
     6541 gcttgtactc ttcttcaaga ttatgacgac acatttcgtc ttccttactt cctgtaaata
     6601 aaattggcat ttctaattta cacaattctt tatgaaacaa agggcgtttc tcgtttgcac
     6661 attgcaatag agcttttgta tcaagattaa caacattctc ccaatctttt ccatgacacc
     6721 attcatagaa ttttcgtgct tgaatatctt gctttgattt ttcccttcca gtgattagat
     6781 tatctatgaa gttatcatta agagtacgtc catcaaagct atctgctata attgcttcaa
     6841 ctaaatcggg acgttctaat ccagtattaa cagctgccca tgctcctcca cttgttccaa
     6901 ttaagccaac tttaccacaa ttcaaatgtt caatcagtgc tattgtctgt aaggcttcat
     6961 catgccatat gtcaggagaa aattgagaaa ctctatccga ctgtccatta cctaaaaaat
     7021 caattaagat acatctaaaa ttttctgcat ataatggcac aagtgcttta aacatattag
     7081 aagaaccagt attaccatgc aggaaaatta tcggtttacc ttgtccatat tctttataaa
     7141 aacaagaatt accttgatac tgaaaatatg acatatattt tcctcctttt tatttgttaa
     7201 gttaattata acaatatagg tatgaatgaa aaacctcatg ttctttctta gaataaaaat
     7261 gtgccacgca taaaacgtga cacatttcta attataaatt ttgctgttta agtattcttt
     7321 gtatgctttt ctctgtcaaa aagtatttct ctgataactg cttgatactc attccatttt
     7381 ggaaatttat acagatttga gaattacgag aagctaagac ttgtttggtg tttgtgtttt
     7441 caccccaatg ctttttgtgc tcttgctttt tgggaatata aatcacttta ccatctatat
     7501 attcttgcac ttgctcaatc agttctttag gcagtaagtc aatcgaattt tgatagctca
     7561 ttttgcacct cctaaataat tattctttag gattagcaaa ggctactgcc actcacaatt
     7621 aaaaataaaa ttttttatta tgtacaatag cctttgctat gcacaagttc caattcccat
     7681 aaactaaata ctcctttctg gtttgaaaaa ttcatctgaa cattgatttc gcttttaaga
     7741 tagagttcaa tgacaaattt acatttttat tatatatatt cagatagata attgcaactg
     7801 tctttgtatg aggatatttt attatagtca atactataat atgtatttat atcatcaaac
     7861 taatatgaac agttgaaagg aatatgaaca ataaaactta gtcaggaaga ataattatga
     7921 agcagacaat agagaaattt gattttaaag ttttattgaa aatttattga caaatttaac
     7981 ttgtaaatat tatactaaac ttaataaata aagtttataa agtaaggtgt tgtctatgaa
     8041 aaatatagtt ggaaaaccgc aaacgataaa aaaagtaaat gaagatttaa taaaaaacat
     8101 tataaaaaat gaaggtccta taacaaaacc agaaatttca agcattactg aattgagttt
     8161 agctacagtt aataagattg tagagcaatt agctttgaaa aatgaagtta aagtaagtgg
     8221 actaagtgaa tctactggag gaagaagagc tcaattattt gaaataaatg caaacttaga
     8281 acacataata gctttatatt attatagaaa ttgttgtata ggggtagttg ccaatttatt
     8341 aggtgaaata atttatcaag aagaatttaa tataagaatg gatacgtatg aaaacgttaa
     8401 ccaagatact ttttttgtta tagatactct aataaaaaag gaaaaaggaa acaatataag
     8461 ggctattgga attggtgtcc caggagttgt aaaaaaaggt gtagttaaaa atataccaac
     8521 tataaaaagt tgggaaggta taaatttaga aaatttaata aagagtaaat acaatactaa
     8581 agtgtttata gaaaatgata caaatattac tactattggg atttatcaaa aacagtataa
     8641 agacaaatat aaaaatatgg ctcttatgta tttagaagat ggtattggtt caggtatagt
     8701 tataaatggt gaattgtatt taggttctac aaattttgct ggagaattaa gttatctaaa
     8761 tatagaattt ggtaagtata actctaacaa cttaaatctt gaagattata ttatgagttt
     8821 aagaaaaaga tatttagaaa gtaaagataa taaatttaag aaagaaattt tatctataat
     8881 atcaaacttt ataaggactc ttgtatgtgt cctaaatcca caagctatga ttatacagtg
     8941 tagtatatta accaagagtg acatggagtt tatagaaaaa gatgtaagca ataatgttgg
     9001 agaagaaaat tttcctaaat taataaaagt agactcattg agagaaggaa gtataaatgg
     9061 agtaattagt atgtgtttaa aagaaactga ctatcaatat tccttatcta ataaaaaagg
     9121 aggatattaa tgtgaaatat ttggtttcaa tagatggagg gggaacaaaa actaaatttt
     9181 gtgtaagtga cttagatgga aacatactta aagaacatac cactggttca actaattata
     9241 agtcagttgg aattaaaaaa acatatgaaa atattaataa tggatttaaa aaaatattaa
     9301 aagatttata tatcgattat gacgatattg agtacactgt atttggtata tctggttgtg
     9361 attctcctaa tgattataag attataatgg atgaaatatt aaaaattggg attaacaaag
     9421 aaaaaatata tctggcaaat gatgcagttt tagcttttta tgcacaagct gattcccctg
     9481 gcttagtaat tgtggctggg acaggttcaa ttatactggg aattaaagaa gatggagaaa
     9541 tatatagagt tggtggatgg gggtataatt ttagtgattt gggttctgga tatgatatag
     9601 ggagaaaatt attaaagaaa gtattgcttt attgtgatga atgtcatgag tatagtgatt
     9661 tatttagttg tgtcttggac ttctttggag caaatagttt tgaacaactt gcatatatga
     9721 ttactgatat aaatgataat gttgaaatag caaacttagc ttcattagtg attgattgtg
     9781 ccgaacaagg tgataaattg gctattgaaa tattaagaga atcatcaact gaactatcta
     9841 aattagctca agtaatattg agaaaaatat ctaatccaat gaaaatggat aaaagcgaaa
     9901 taaatatagt tttgtctggt ggtacattaa ataaaactgt atatgctcaa atgttaatga
     9961 ataatttaaa aacagaaaat acagataaaa atttaaaatt tatatctcaa gaaaatcagc
    10021 cagtatatgg ggggataaga cttgctatgg cattagctaa taggagggtc aagcatggat
    10081 agaaaaaagg taatagtaca aatttttact ggaggtttta aggctaaaga aataaagttt
    10141 aatgatataa aatgtaaact actagaatta ttggactgta ttgatataga aaaggttatc
    10201 atgggatgga gtgtagatga agaattatat gtagaaacta aaaaactatt aaataaatat
    10261 aatgttgaac tatatctttg gttgccatgt ttttctgaaa taggattact tgaaaaaagt
    10321 aatttactaa ttgattatat ggggaaggaa gtcaagggct atgctttaca agaggatgaa
    10381 aattttgagt tttattgccc aaataataag gaaaatataa aaaatataaa gaaaatatat
    10441 agtacttatt ttagtgatat taaatttgat ggaatttttt tagataaaat aaggtatggt
    10501 tctttttcga ataaactaaa tggtgttttt aattgttttt gtaaagattg tttaaatata
    10561 tataaaaaaa ataatattga taaagagtcg ttaatgagag aaatgaataa tgtgtctaag
    10621 ggagttatag ataatgaaga tactccattt ggtataacag aatacattaa tggaaaatac
    10681 aaatttaaaa ataaaatatg ggaagatttt tttgaaacaa aaaatcaaaa tatatacaag
    10741 gcattattag agataagtag ttattttaaa agtaagggat tgaaaatagg tatagatgct
    10801 ttttctcctt ttatttctta ctttacaggt caagatttag aaaaattaca agaagtagca
    10861 gattttataa aaccaatgat gtatagaact acaaaagctc cagctggttt accatttgaa
    10921 tttgaaaggt ttattgatga atccatacat aagaataaag agattggaaa aagaaatttc
    10981 ttagatattt taggaattaa ggagtatgaa ggagatgtat atcctataga atttgtacta
    11041 gaggaaatcg attttatgac aagaaatctt aataaagata tatatgtagg agttgaaata
    11101 aataaaaagg aaattatatc tcctgttaca cctaagtaca taagtgaaaa tatttcaaat
    11161 ataaataaga caacagtaaa aggatatgtt ttatcttggg atttgttgtc agcaccaaaa
    11221 gaaaatattg atgaaattat aaaatggttt cggaattagt gaggtgataa agtgagtaag
    11281 atattggaaa tacaacaaat atataaaagt tttggaaagg tagatgtttt aaaagggata
    11341 aattttgagt tagaaaaggg agaagtaaga gctcttttgg gagctaatgg agcaggaaaa
    11401 tctacactta ttaaaatttt aggaggagtt cattctcaaa ctaaaggtaa agtaattttg
    11461 actggtaatg aaataatgtt taaaaatcca catcattcta aagaaaatgg tattagtata
    11521 atttatcaag aattaagctt gataccaacg cttacagtac ttgaaaatat ttttcttggt
    11581 agagaaattt taaaaggaag tgtgttttta aacaagaaag acatgcttga ggaatatatg
    11641 aaaatttgtg aggattttga ttttaatata gacccacata tagtagtttc taatttaagt
    11701 gtagcaaacc aacaaatggt tgagataatg aaagctgttt catgtaattc tgaaattatc
    11761 ataatggatg aacctacaac atcacttact aataatgaaa aagatgggct atttaaaata
    11821 ataagtagac taaagtctaa aggtaaaaca atagtctata tttctcacat attagaggaa
    11881 gtttttaaag tatgtgatag tgcaagtata atgaaaaatg gaattatgat tggaacttat
    11941 gatgtagagg atttaactaa attaaagata actcaactta tgacaggaaa tgataatcac
    12001 aaacttattg agaagaagga ttatacattt gcaaaatatg aagagccacc agtactagaa
    12061 gtaaagaata taagctggtc taacaaaata aaaaatgttt catttaaagt ttatagaggc
    12121 gaagttgtag gtcttgcagg tcttgtaggt tcaagaagaa ctgaaattat aaatttgata
    12181 tttggtatag ataataaaga tagtggagat atttttatca atggtaaaaa agtagacata
    12241 acttctccta aggttgctat aaaaaacaaa ataggattga ttcctgaaga tagaaaaaat
    12301 ctaggattaa ttttagaaca agctatatac acaaactcat catcagtaca aattgataaa
    12361 ttcaagaaat ttttgtttat agatagaaat aaagaaattg agttttctaa aaaaggtgtt
    12421 aaggaattag gaataaaagt tagtgatgta aagcaagaag taaaagagct tagtggtgga
    12481 aatcaacaaa aagtagtagt ttctaagtgg ttagagaaag atttagattt aataatatat
    12541 gatgagccga caaagggaat agatattgct gcaaaagaag atattttcaa tacaatcaaa
    12601 gaattttcaa gtaaaggaat aggagtaata tttatatctt ctgatttaga agaagtaata
    12661 agagtaagtg atagaatttt agtaactaga gaaggttcta ttgtagatga tttaaaaaat
    12721 gagaatatac aggttcaaga tataatgaac agtatattta atgtataaaa aatgaaaatg
    12781 gaggactgag atgaagaaag cttttgagaa gttaaattta agagattatg gagtattatt
    12841 aggtttttta gctttatgta tagctataag tattgcaact ccatcatttt taggtaaaca
    12901 aaatatactt aatcttttac gacaatcttc tataattgga ataatttcag caggtatgac
    12961 ttttgttata atatctggaa actttgatat atcagttgga gcagtagcag ctcttgcagg
    13021 tgctataaca atgaaatttg cgactggtgg aactaatttg tttgcatcca tgttcttagg
    13081 tgtagcgatt tgtgcagtaa taggattaat caatggtata gtagttgcaa agataaatgt
    13141 accatcatta atagccacta tggctatggt cactattgta agaggattgc tgttgatgct
    13201 tacaggagga tatccaatta ctgaaaatat accaatactt gattacattg gaaatggata
    13261 tttgtttaat ataccaatac cagtagtaat tttctttata gtggtgttag cttcgtttat
    13321 tgtacttaat aaaactaagt ttggtagata tgtttattct gtaggaggga atcaagaagc
    13381 aagtaaactt aatggtataa atgtagattc tcacaaagtg aaagtgttta ttattaatgc
    13441 tgtacttgca ggaatagctg gaatcgtact aacaggaaga cttgggactg caactgcgat
    13501 tgcaggtgaa ggatatgata tggatgcaat tgcatctgta gtaataggag gtacatcagt
    13561 ggcaggaggt tctggttctg tacttaaaac tgttatagga gtattactta tgagtgttat
    13621 aaacaacagt tttaatcttc ttggaataga tgtatatttc cagtacatat ttaaaggatt
    13681 aataatttta gcagcagttg gatttgattc ttatagcaag aaaaaacttg cttcaagata
    13741 aaaaataagg gggatttttt tatgaaaaaa ttaatgaaac atttagcatt gttgctatca
    13801 cttgttatga tatttggttt agttggttgt tcaaatggtg gtgacaaaga aaaaaatgga
    13861 gaaaagaaaa tagcagtatt actaccgggt tcaacaggtt actttgtagc aactaaacaa
    13921 ggtatagatg caaaatctaa agaattaggt gtaagcgtgg aatatgcaga tgctcaatgg
    13981 gatgctagta aacaattatc acaagctgaa gattttatgg ctaaaggggt agacatgatt
    14041 gcattatgtg gagttgattc tgctgtaagt gagagaatag ttaaagctgc taatgattca
    14101 gacgttccta tagtagcatt tacaaattct ataggaagta acccaactgg agaatttaaa
    14161 ggtttagtta catatatagg acaaaatgag gaagaaacag gagcattaac tggaaaaatt
    14221 gctaaaaact tattaggaga aactggtggg aaagctgtat taatagaagg agtaccagga
    14281 actactccac aagtattaag aaaaaaaggt cttgaaaaag aattaaaaga tagtaatata
    14341 gaaatagttt ataatcaaac ttcaaattgg gagaaagagc aagctttaaa ggtaacagaa
    14401 gatttaattc aaaagaaaac tgattttaat gttataatat gtcaagatga taattctgct
    14461 acaggtgcag gtcaagcttt aaaagatgct ggtcttaagg ataaagttaa agtcataggt
    14521 ttgggtggaa gtaaagatgg attaaaagct ataactgatg ggttaataga tggtacaact
    14581 tatatgtctg ctgtagaaga aggtggatta gtcatagaaa aggcatctaa attccttaaa
    14641 ggtgaaaaaa tagaacctgt tacacaaatt aaacaagttg aagttaacaa agataatatt
    14701 tcagagttca agggtgaatg gtaaattgtc atagataata aataagattt ttagagttac
    14761 tttagaaact atataaagta actctttttt taatttaatt tagtgataaa ttattgaata
    14821 gtatttctaa acaaataaaa aattaatata aacaaagact taattatact aaattataag
    14881 taaaactaat ataaacttat ggaaaaaagg aaaacgtcta aagtattgga attactatag
    14941 tgtaggtgtg gtattaaaaa aacataaaaa aacataaaaa aattgttgaa aaaaatatag
    15001 aaattttgta taattaaaga tttgaaaagt gcggtataca aagccaattc ttttgtatat
    15061 aatatatata aattcagaaa aaggaaggga tatgtatggc caggcggtta tttttagaaa
    15121 ggattggact tatattttta ggttcagcaa tattagcatt tggagtttat aatttttatt
    15181 atttaaacaa cataacagaa ggtggagtgt taggtatttt actactgtta aaaaatttat
    15241 tcaatataca accagcaata gcaaatgttg taatagatag tttactactt ttagtaggat
    15301 ataaattttt cggtaaaaaa ttccttatat attcaatagt agcttctata actttttcag
    15361 tgttatatga cttatttgaa gctataggac ctctagtacc acaatcacaa aatatgcttt
    15421 tatcaactat tttggcaggt gtaactgttg gaactggtgt aggtatagta gtaaaagcag
    15481 gttgtgcatc aggaggagac gatgctttag cattagtaat ttctaagact acatctttga
    15541 acatagggca agtttattta gcaacagatg ttattgtatt gttgttatca ttattttatc
    15601 tatcagcgtt tgatatcttt tattctttga ttgctgttac aattagtggc aaggtaattg
    15661 attttattta ttatcatggt aaaagtttgg atatggatat aagtaatgat atagttccag
    15721 aatgttaata taaattaaat aactttagat tttattaggg gtggcagatt tgccaccttt
    15781 tttttgtcta aatctttata gaaagtataa tcccatctag gtttttgcaa atctcaaata
    15841 tagaattatg ttggaatgac tgggaatacg attgctagat gtgcatttta aaaacccaat
    15901 tttatattta tgaaatacta atcattaatg gatgtttctt aatcctatct ttatacctaa
    15961 aatgataaga taaatgaagt agcaatacaa agctaaaatt tgtaaaagag attaaattaa
    16021 atggaggagc taaaatgttg gatatagtta tgatagcatt gttattcata ggatttgttt
    16081 ccatgaagtt acttgctgat tggtgtggga aacaaactga aaaaaaataa tgtaatgtat
    16141 ggagggatta cctatgtggt ttctggcagt tgtaattata atgttaataa tatatttggt
    16201 atatgcgctt attaatcctg aaaaatttta aacagtataa ggctaataag taagttgaat
    16261 tggggggaac aatttttatg gtgcagatag taatagtgtt agcgattttt atgattttag
    16321 taatacctat gggcaagtat ttatatcata tagctactaa tcaaaaaaca tttggagata
    16381 gactatttga taaagtggac aattttatat ataaggtatg tagtatagat aagaaaaaag
    16441 aaatgaactg gaaacagtat gcattagctt tattatttac aaatgcagtt atggtattta
    16501 taggatatat catacttagg actcaaagta tgcatatttt taatccaagt ggaattaaaa
    16561 gtatggaaca aggactatca tttaatacaa taataagttt tatgacaaat acaaatcttc
    16621 aacattattc aggtgaatca ggactttcat actttagtca gatgacagtg ataatatata
    16681 tgatgtttac atcagctgca acaggatatg cggctgctat ggcatttgta agaggtctag
    16741 tcggtaaaaa gaaaacattg ggaaattttt atgtagattt aattaggata acaacaagag
    16801 tacttttacc aggagcttta attattgggc ttatattagt tacacaagga gtaccacaaa
    16861 cttttgctgg gacagaaaca gtgactacta tagaaggaaa gcttcaagat atagctagag
    16921 gtcctgtggc agctcttgaa tcaataaaac atttaggaac aaatggtggt ggattctttg
    16981 gttcaaattc atcacaccca tttgaaaatc caacaataat atcaaatata gtagaaatat
    17041 tatctatgat gatattacca ggagcttgtg ttgttgcatt cggtcacatg ataaagaata
    17101 aaaaacaagg ttgggttgtg tttggcgcaa tgtcaatcat atttttaata ggtctagtag
    17161 tgtgctttaa agcagaaagt gcaggaaatc ctattttatc acaattaggg cttaatcaaa
    17221 gtatgggaag tatggaaggt aaggaagtta gatttggtat agcacaatca tcattattca
    17281 caacagtgac tacttccttt acgacaggta cagttaataa tatgcatgat acactgactc
    17341 ctttaggagg attagtacca cttttaaata tgatgctaaa tgtagtattt ggtggtaaag
    17401 gcgtaggtct gatgaatatg ttgatgtacg ctataatagc agtattctta tgtggattaa
    17461 tggtaggaag aacaccagag tttttaacta agaaaatcga aggaaaagaa atgaagctta
    17521 ttgctttact tattatatta catccacttt taatactaat gttctctggt ctttcagtag
    17581 caataccagc aggacttgaa ggaatttcaa atcctggatt ccatggatta tcacaggtat
    17641 tgtatgagtt tgcatcttca gcagccaata atggttcagg atttgaaggt ctaggagata
    17701 acactatgtt ttggaatata acaacaggta tagttatgtt ctttggaaga tatgtgtcta
    17761 ttatagtact actagcaata tcaagtttgt tagcatctaa aaaagctgta aatgaaagta
    17821 taggaacact tagaacggat aattttacat ttacaatagt tttagtactt gttgttttaa
    17881 ttgttggtgc tttaacattt ttcccagcct tagcgcttgg acctatttcg gagcatctag
    17941 tgttgtggca ctaaaaaatt gggagggagt aaagttttat ggaaactaaa aaaacaaagt
    18001 ttattacaaa agatattttc aaaacttcca ttatagaagc ttttaaaaaa ttaaatccta
    18061 aatatatgat gaaaaatcca gttatgtttg ttgtagaaat tggatttata gtaacattga
    18121 tacttacaat agtacctagt ttgtttggtg atgaaggcaa taacttaaga gcttataatg
    18181 ggattgtaac aataatctta tttataacag tcctttttgc aaattttgct gaagctgtag
    18241 ctgaaggaag aggtaaagct caagcagata cacttaagaa aactagaaaa gatacagtag
    18301 caagggttat agatacgtta ggaaatgaaa aaatgataag tgcaagtgag ctaaaacttg
    18361 gtgatattgt tttagtaaat gctggagaag ttatcccaaa tgatggagaa gttatagagg
    18421 gtatagcttc tgtggatgag tcagcaataa ctggagaatc tgcacctgtc atgagagaat
    18481 caggtggaga tttcgcatct gttacaggtg gtactacagt tgtaagtgac tggttaaaga
    18541 taagaataac aacaaaacca ggagaatcgt tcttagataa aatgatagct cttgtagaag
    18601 gtgcttcaag acaaaaaacg ccaaatgaga ttgctttaaa tactttgtta gtagggttaa
    18661 cacttatatt tatggttgtt ttaatatctc tttatccaat ggcaaactat gcaggtgtaa
    18721 agattccaat ttctactatg atagctcttt tggtatgttt aataccaact actataggtg
    18781 gattgttgtc agctattggt attgcaggta tggatagagt aactagattt aatgtaattg
    18841 caatgtcagg aaaagcagta gaagcttgtg gagacgttga tactatgatt ttggataaga
    18901 caggtacaat tacttatggg aatagacttg catcagaatt tatagcagtt ggaaatgcag
    18961 attcaaaaga tttaataaat tattctgtta tgtgttcatt aaaagatgat acaccagaag
    19021 gaaaatctat agttgattta ggtatgaaat taggttcaac aggtaagaca aaagaggcag
    19081 aggaagctga gtttgtagaa ttttctgcac aaactagaat gagtggtatt aacttagctg
    19141 atggtagagc tataagaaaa ggtgcttatg atagcataat aaaaagagta aaagaatctg
    19201 gtggtacagt gccagaagat ttagaagaaa atgtaaatcg agtagctaaa ctaggaggga
    19261 cacctttagt agtgtgtgta gataatgaga tatatggaat tatctactta aaagatacag
    19321 taaaatcagg gcttgtagaa agatttgcta gacttcgaga gataggaata aagacaataa
    19381 tgtgtacagg ggataatccc ttgacagcag ctacaattgc aagagaagct ggagtagatg
    19441 gatttattgc agaatgtaaa ccagaagata aaatagatgc aataaaaaaa gaacaattag
    19501 agggtaaaat agttgcaatg acaggtgatg gaacaaatga tgctcctgca cttgctcagg
    19561 cagatgtagg tcttgcaatg aatagtggta caacatctgc aaaagaagca gcaaatatgg
    19621 tagatttaga ttcagaccca actaagatat tggaagttgt tgaaatagga aaacagcttt
    19681 taataactcg tggggctttg acaacattta gtatagcaaa tgatgttgca aaatattttg
    19741 cgattatacc agctatattt actcttgcaa tacctcaaat ggagatatta aatgtaatga
    19801 aactttctac atcatttagt gcgatattat cagctttaat atttaatgct ataattatcc
    19861 catgtttaat acctcttgca atgaagggag taaaatatag acctatgaaa tcacaagcta
    19921 tgttaatgag aaatatgctt atatttggtc ttggtggagt tatagtacca tttttaggaa
    19981 taaaattaat tgatttattg attacacctt tagtaatggt tcttaattta ggttaataaa
    20041 tggaggatat aaaaatgaaa acattaaagc cagcattact tgtaagtatt gtattgttag
    20101 ttgtttgtgg acttgtatat ccacttgttt tgacaggtgt aagccaagtt gcatttaaag
    20161 ataaagccaa tggaagtatg atagaagtaa atggtgtaaa agtaggttca gaacttattg
    20221 gtcaaagctt tacagatgtg agattcttta agggaagagt atcatcagta aactataata
    20281 cttatacaaa agaagattta gtaccagata aagatggaaa cacatcttat ggaggtgtat
    20341 catcagggtc ttttaattat ggagctacaa atccagaact tcatgataga gtacaaaaag
    20401 atatagaaca gtttttaaag gataatccaa ctgtcaaaag agaggatata ccaacagatt
    20461 tacttacagc atcaggttca ggacttgacc ctaatataag tccagcctct gcaaaaattc
    20521 agataccagc tatagcaaaa gcgtctggaa tatcagaaag taaattacaa aaaattgtag
    20581 atgataatac ttccaaaaaa ctttttggag ttttaggtga agatagagtt aatgtattaa
    20641 aggttaatgt agaggtcgca aaaatattag gtcttatata gtattttgtg gaattttagc
    20701 aacaaaatat tacgcaagat ttaatttaaa ggttaaattt taaagagtga aaatcgataa
    20761 tgattttcac ttttttattt gaatattact aatatttgga ataaagtgat tgtaagtata
    20821 aaatccaaaa aaaggttgca ttatcaaaat gataatacta taatatattt atcgttatca
    20881 atttgataat agatttggac atataggagg aaatagtatg ttatataata acgcattaga
    20941 gttgatagga aacacacctg tagtaaggct gaataattta ggctgtacta atgggtgttc
    21001 taacatatat gtaaaattag aaaaagtaaa tccagcagga agtataaaag atagagctgt
    21061 ttatgaaatg ttagagggct tagagcaaag aggagaatta aaaaagggag atgtactagt
    21121 tgaagcaact agtggaaata ctggtattgc attatctatg ataggaaaat taaaaggata
    21181 tgaagtagta atcgtaatgc ccgaaacaat gagcgttgag agaagagatt taatgaaggc
    21241 ttatggagca aatttggtgc ttacagatgg taaagctgga atggcaggtt ccatagaaaa
    21301 agcaaatgaa cttttaaaag aaaatccaaa ctataaaagc ctaaaacaat ttgaaaataa
    21361 agataatcca aatgcacatt ataaaacaac agcagttgaa attatgaatg atgtcaaaga
    21421 tttagatata ttcgtatgtg gagttggaac aggtggtact ttaataggaa ctgcaagata
    21481 tttaaaagaa caaaatccaa atataagagt aattgcactt gaaccaaaaa aatctccagc
    21541 tatatcagaa aacaagactg gacctcataa aatacaagga ataggtgcta actttgttcc
    21601 tgaaaattat gattctagtg tagttgatga agtaatatta gtagatgatg aagatgcatt
    21661 tgatacagtt aaattacttg cagcaaaaga gggaatttta gtagggatat cttctggagc
    21721 aaatatattt ggtgctatag agatggcaaa aaagtatcca gataaaaaga tagttacagt
    21781 agctccagat ggagttgata agtatatgtc tatgggaata ttttaataat tttatttgag
    21841 ggagtgaata ctttgtttaa gaaaataaat aaagatattg aatatataat gaagaatgat
    21901 ccagcagcta ggtctaagat agaagttttt ttactttatc catcagttca tgcaatgata
    21961 atgcatagga tggctcatgc tctttataag aagaagaagc tttttacagc aagattaata
    22021 tcccagatat ccagatttat gactggcata gaaatacatc ctggtgctaa aatgggggaa
    22081 ggtatattga tagaccatgg aatgggtgtt gtaattggag agacagcgga agtagggaat
    22141 agggtgacaa tatatcaagg agctacttta ggagctacag gaaaagatac tggaaagaga
    22201 catccaactg ttggtgatga tgtattaatt ggtgctggaa caaaaatact aggaccttta
    22261 aatataggtt caaattcaaa aattggtgca aattctgttg tcgttaagga tgtacctaat
    22321 ggtgcaacag ttgttggtat accagcaaaa atagtaaaaa taagaaacct agaacctgta
    22381 aaaaagaata aaaaagaagt gtcctatgag tatgatgaat tagataatgt atattatata
    22441 taggatttat ataactataa aataaacttt ataaaaatta ttttaaaagg tatttttttc
    22501 aaatttgagg acataataaa aaagaactga atgttacagt tttggttcat caatctttga
    22561 taaacaagat tttaaatttg tagcgtctag gaggatttta ttatgaaagc aaatgttaat
    22621 caagatactt gtataggttg tggattatgt ccatcaatat gtccagaagt atttgatatg
    22681 aaggatgatg ggaaatcaca tgttatagtt gatgaagtac caagtgatgc tgaagaatca
    22741 gcagcagaag ctagagaaag ctgtccagtt gatgcaatag atgtgcatta ataaaataaa
    22801 tgcctataaa aattgaattt tcaattttta taggcatttg tcttttattc agttagtatt
    22861 tcaacacctg tttctgtaac taaaaccata cattcccatt gagcagataa tttaccatct
    22921 tttgtataaa tagtccaatt attttcttca tcaacaaaaa tttcgtgagt tccttcattt
    22981 accataggtt ctattgtgaa tatcatccca ggaaccaata gcatcttagt ccctttttta
    23041 gatacataac ttacaaaagg gtcttcatga aagcttaatc caactccatg accacctatt
    23101 tctctaacaa cagagtatcc atttttcttt gcataagagt tgatagcatc acctatatct
    23161 cctaaaaatc cccatggttt ggcaacttca agaccttttt ggacacattc ctttgtaact
    23221 tgaactaatt tttttgcatt ttcattaaca ttacctatca taaacattct tgaagcatct
    23281 gaatagtatc cattaaaaat agtagaaaca tctacattta taatatctcc atcttttaag
    23341 ataatgtcct tacttggaat accatgacaa acttcattat ttactgatgt acatacactt
    23401 ttaggaaatc cattatagtc tagtggagca gggatagctc cattttgtag tgtaaagtca
    23461 tgaaccaact tatttatatc ttctgtagac attccaattt cgattttatc tgaaatataa
    23521 tccaaaattt ttgtgtttat tacagcactt ttttttattt cctcaatttg actttcattt
    23581 tttataatat catgagttgg tacaatatga cctaacttag caaatgaatc tattttatta
    23641 tccatatcca tatgacattt tttatatttt ttagtacttc cacaccaaca gctatcattt
    23701 ctatttaact tcataacttt tcatccttct tttattattt atttaaatta tgggttacat
    23761 aaactatttt aaaacataac cattataaat attataacat aaatactaag aaatccttta
    23821 tagatagtga tttattcaaa gtataaatta ttatatggtt attgttgatg aaaagaggat
    23881 agttaaatgt attattggat attagcgcac ttaatagttt aattcaagtt tttcaagaaa
    23941 atcatatgtt agaggacaat tttcctttaa agtatttttt gaatcttcat caagataata
    24001 atgcacaaat gattctgcaa aaaattcttc tataaacttg cttatatatg catgtgcaac
    24061 ttgcttaggg tcataaagct tatatctttc ctgagcaaat acttccataa attcttcaga
    24121 atttgatttt ttatttaaaa catttttatc tatagcatgt gcagtttcat gtaattctaa
    24181 attttttgat gaatgtacat ttgcatagct agggttacta taacctattt tagccactat
    24241 agaattattt ctatatatac caggtacatc tttccaagtc ttgcctgatc ttacccaaca
    24301 tggaggtaat tgatatttta aatcactaaa ttctggttca tcagttagat ttgaatttat
    24361 taattttatt tttatatcct tttctttaag tgtctgtaaa tatttaatat ttatttttgc
    24421 aagtctgttt ttcatatttt caacttcatc tttatcataa ttcaagtcat caggaagtat
    24481 aagcatttgt tcaattatat ctatactact gtcttttata ttttcagatt cttcattaaa
    24541 tttatattga agagtattat tttcatagat agttatattt gtattatttt ctatagaata
    24601 agagtgaata tcctctaaat ctaaaatatt catttgttgt aaaatatgtt ttgtttcgcc
    24661 actccatact actaaagaaa gtgaattaaa attaattagt ggattttctt ttgcttttat
    24721 atcattgctt atatctacat caaatacata ttcaattgag tcttctatgt taagactatc
    24781 tgtattcttt ttaatatata aatacgcttg ttttaagtct atataaatct catctaaaga
    24841 ttcgtcataa aattctgata aattattagt aaagagttct tcacttaaaa gttcattttt
    24901 atcagttata ctttcaatta aaatattaat ttcttttatc atatcgtaaa tacttatttt
    24961 tctatttgct ttttcactag atacattgca tggcgcaaga gcaatgcaat aaaaatcttt
    25021 ttctttttta taaaaggctt taagtgattt tgataaaagt gtaaagtcta cacctatctg
    25081 taagttgacc ttaagtgagt cattttgtaa taattcagta ttatttcttt tagctgtatt
    25141 taaattgttt tccataatc
//
LOCUS       DAELLV010000029        24912 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.029, whole genome shotgun sequence.
ACCESSION   DAELLV010000029 DAELLV010000000
VERSION     DAELLV010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24912
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.029"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1215
                     /locus_tag="KPD58_001042"
     CDS             <1..1215
                     /locus_tag="KPD58_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264825.1"
                     /translation="KIFSNIDNLTYVNFIEELDKSSATAKQKEDIKKDFKNRRFPYVR
                     NSGKYGLNTSLENMETNVKRIVDTVIKSNIEIDEIDKLDLTQYEEKDSVCNVCNINKT
                     TKLDIDLRERKDSKYNFLFRGAEKSGFKRSGQVESNICFECEFFNLMCLLYINLKRPM
                     VFAYTNDLRELAFLNHKIMLKRKMYSDKSFYKKLLHEKISSIRLYRFDIVKNKGIILK
                     FDSIIEYKELLKKIELIDIIDNYNFSREPVNTKNLGKKMIDNGNLSNLKELLLDNISF
                     LKQSTGTSREMDFVSSSYNIGLYIKLCWIVDGGEEYKMKNYMESNLIYSRVGKDLFNK
                     MTDESRRKNFSMKVIQLLKSNDRTQLFQTIMHVLVSNGILIGEGFVEGLMQSSELELN
                     TNVGLFIQEIMK"
     gene            1235..2248
                     /locus_tag="KPD58_001043"
     CDS             1235..2248
                     /locus_tag="KPD58_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DevR family CRISPR-associated autoregulator"
                     /protein_id="HBG0264826.1"
                     /translation="MNEKRVKGVTLTILTESPVPLSYDQGYGNYTPIKKEQYREKIHA
                     KTSIATITYDLRRMLHQEYGWNLSNIVFGKKGNIYPSIKKNVGCVDENGLETDVFGYL
                     IPLKDKGSISKASPLRIIPFRSLNPYIGSTQLITNRGFLSSEFGRKYYDEKEENEVPR
                     DENFPTTQALAIEETLSDYYVYTITLELDRIGVVEVEDGKLLLPEERKFMSKELREKA
                     VKDILDAITVFTRNIKHSSVLLKPLAVIGGAFDKVVPFFWDDVDYNADSGEINLEGVI
                     ETIESYSLKESNTILAINDRLKISNKKELNKFNLSKYPVKEIKNLADRLEIGEDNMWY
                     LKE"
     gene            2264..3061
                     /gene="cas5"
                     /locus_tag="KPD58_001044"
     CDS             2264..3061
                     /gene="cas5"
                     /locus_tag="KPD58_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HBG0264827.1"
                     /translation="MKVLQCKLKQPTAHYRDPKVFQNEYISTLNLPSKTTIMGMITYL
                     CDRRLNSDIDIGIIGTHHHRELEFSRGENIDFWNEYTNMRKGKDKEKFLLQGNYYDYY
                     KEHKAQNSILNYEVLKEVELTIFISCKDDEELEFIKKKLESPCKYANLGRKEDFVIPS
                     EKGCFVKEVELKEVIPMNTRDAIKENIKLKNTYVRVDLRDKENVETIINQGVLIALPH
                     KYKDLEANRDDRVYEFCHYIYVDNDGIYPKNIKVNVCIDTKEVFTWL"
     gene            3088..5496
                     /locus_tag="KPD58_001045"
     CDS             3088..5496
                     /locus_tag="KPD58_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HBG0264828.1"
                     /translation="MKNIMQKEILAKSYNLRTNQQEQTLVEHIKDLFEVLESILELNL
                     YSDKDVEILKICCALHDLGKINSIMQQKMEINNKISYSCSEEERKKLESDKKSLKKIA
                     RHNIFSGAFLKDILEKMNLSEEDKLYIYKSIMLHHGNYEDYMRLSISKVQEEIYEYIE
                     KGILEKEEFNLKDIESYINNILNVDFKFGEDVLDYDYIDKLSESMFIDSDYNNGQIDD
                     SVLNYRKFKYILYKGMLNLIDHSASSRQKGIKFYNDFTDEEIDNMILNEIYKSQGNLE
                     KNNIEFNTIQKRLRELSGRNVLTVAFTGSGKTAADYRKTFKRKFFLVPNKISAESFYR
                     KNIFQNKNDLDTRSSNDYIGLLHGDINLYSENEDNGEHDFVLTLRDIDLSINFCKPCV
                     IATVDQLLLSMFKFPGYEKIFAAVKNASITVDEVHLLEPKMFLILIYFMQFACKYLDV
                     DFHLMTATLPKSYKEQMINKGIIFQEESNKNVTDKGEIVFIESNKEEEICEGKDVKVS
                     FIKEKEIKSIVEDALENKQKILIIKNTIDSVNRTYEFLKENLSDKYGGVDIDVLHSRF
                     KFKDKKEKYSKILNGEGDIWISTQSVEISLDLDFNIIISDLATMDSLIQRMGRCNRNN
                     KYEYGNFYILPSEDKIYDNKLKNTTKNILKDILKTNSIFTMGIRKTILDDYYDNSVVK
                     KYFEENFISCDKEIKNIYGINKEIFDGLDLIFNFEPYKNIVDSKKEAAKIFRDIDVSY
                     KIILEEDFYKEDRELQQDSIQVSGFIFNRLYYYGLISKVEGYMVLKSSSKFEYNSTVG
                     LILK"
     gene            5937..6884
                     /gene="prmA"
                     /locus_tag="KPD58_001046"
     CDS             5937..6884
                     /gene="prmA"
                     /locus_tag="KPD58_001046"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBG0264829.1"
                     /translation="MNNWIEVTIKTTTEAVEPITNILYEQGAGGAVIEDPKDFLFQKK
                     NELDWDYVEEEVFKKNEEDDVLIKTYVSEEKNVMEFVEIIKQKVLGLKDFGIDIGEGS
                     VSLDQVNEADWANAWKAYYKPTKVGQRVVVKPTWEDYAMQEGDLIIELDPGMAFGTGT
                     HETTSMCIRELEKYVNKDSKVFDIGCGSGILAIAAAKLGAKEVVAVDLDEVAVKVAKE
                     NVLENKVEKSVSVMHGNLTDVIKDKADVIVANIIADIIKILAKDVQNFMKEDAIFISS
                     GIILDKVEEVKESLIENGFEIVEVQKLGEWSAIVSKLKK"
     gene            6903..7661
                     /locus_tag="KPD58_001047"
     CDS             6903..7661
                     /locus_tag="KPD58_001047"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBG0264830.1"
                     /translation="MDRFFVEKNNINLQDKTCTIEGEDVKHISKVLRCKLGEKLEICD
                     KNNNEYICEIMNIDKSIVNLEILEKVDINRESELKVRLYQGLPKAPKMEMILQKLTEV
                     GVEEIILVQTKRSVVKVDDKKEDKKFERWERIIYEAAKQSKRGKIPKLRGVLSFKEAL
                     EDMKKNNVNICPYENERTVSIKHALKKCDSNIDSVGIFIGPEGGFSEEEIEQIQKNNC
                     NVVSLGPRILRTETASVVASTIALYELSDLGGEK"
     gene            7663..8961
                     /gene="mtaB"
                     /locus_tag="KPD58_001048"
     CDS             7663..8961
                     /gene="mtaB"
                     /locus_tag="KPD58_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HBG0264831.1"
                     /translation="MKKVAFYTLGCKVNQYETEAMLELFEKDGYEQVNSEEYADVYVI
                     NTCTVTHMSDRKSRQYIRRVKKKNPDAIIAVVGCYSQVSPEEILDIEEVNLVMGTNDR
                     RKIVEEIKKINSSKKVSTVDDIMKVKAFEEIEISQTNGKTRAFMKIQDGCDRFCTYCI
                     IPYARGRVRSRDIDSIVDEVKKLANNGYKEVVLTGIHVASYGKDLKDRDIKLLDVIKQ
                     INQIEKIERIRLSSVEPILFTDEFVNEVLKMDKVCPHYHLSLQSGCDETLKRMNRRYT
                     TLEYKTIVDRLRSKMPDVAITTDVIVGFPGETNEEFKKTYEFLKEIELSQMHIFKYSP
                     RKGTPAATMENQVDPQMKHFRSEQLLNLSKVNFNKFATKFIGRELDVLFEQNIEGNKY
                     EGLTSNYIRVVVESDKNIQGQILKVKINDVKDEYVEGILL"
     gene            9004..9354
                     /locus_tag="KPD58_001049"
     CDS             9004..9354
                     /locus_tag="KPD58_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HBG0264832.1"
                     /translation="MDCIFCKIANGEIPSTKVYEDDRVLAFNDLNPVAPYHILVVPKK
                     HYDSLIDIPDKEMDIVSHIHVVINKIAKEKGFDQTGFRVINNCGSDGGQEVKHLHYHI
                     LAGKKLPNYEAGQN"
     gene            9517..9696
                     /locus_tag="KPD58_001050"
     CDS             9517..9696
                     /locus_tag="KPD58_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBG0264833.1"
                     /translation="MSEVRVRENETLDSALRRFKRQCAMSGIMSEVRKREHYDKPSVK
                     RKKKAEAARRKNAKK"
     gene            9726..10169
                     /locus_tag="KPD58_001051"
     CDS             9726..10169
                     /locus_tag="KPD58_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HBG0264834.1"
                     /translation="MSLKQKLQEDLKSSMKNKDTVRKSVVTLIRASIKQYEVDNRVEL
                     DEDGIIDVIAKQLKQRRDALVEFEKAGREDLIKETEGEIEVLKEYLPQQLSEEELEEI
                     VKSTISEVGATSMKDMGKIMSVIQPKVKGRADGKLINKLVKQNLQ"
     gene            10280..10777
                     /locus_tag="KPD58_001052"
     CDS             10280..10777
                     /locus_tag="KPD58_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HBG0264835.1"
                     /translation="MIISVKEIRNFLINIFIPLIIGYLSSILTMIISGTDISTYYLQL
                     TKPSFAPPAFIFPIVWTILYILMGISSYLILRKGYNLPKVRDAIFYYGLQLVLNFIWS
                     ILFFGFGLRFTALIDIIIMILVSIIMISKFSKIDKRSGYINLIYLMWLVYAGFLNYFI
                     WFINK"
     gene            10970..11533
                     /gene="raiA"
                     /locus_tag="KPD58_001053"
     CDS             10970..11533
                     /gene="raiA"
                     /locus_tag="KPD58_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBG0264836.1"
                     /translation="MQIIVSGRQMKLTDGIKGYVDGKLSRLEKYLDPESEVKVTVSAK
                     KDRQKVEVTIIPINGQIIRAEDVEDDLYAAIDIVCDKLSRQVVKYKTKVKDKVQNNKS
                     IRFENLDFIDNSSEFDDYDYDDEEDENIVIERRKKFNVKPMSSEEAILQMELVGHNFY
                     MFRNQDNFEINIVYKRKAGGYGLIEQD"
     gene            11746..11982
                     /locus_tag="KPD58_001054"
     CDS             11746..11982
                     /locus_tag="KPD58_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HBG0264837.1"
                     /translation="MLEISTDLQVNQPVITVTSNTFISIENYLSILEYEVDLIRIKTK
                     VKTIKISGDKLSLKYITDSEIGIKGIIYNVEYVD"
     gene            11995..13338
                     /gene="yqfD"
                     /locus_tag="KPD58_001055"
     CDS             11995..13338
                     /gene="yqfD"
                     /locus_tag="KPD58_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HBG0264838.1"
                     /translation="MISFIRGYYVIVVEGVGLEQFLNHLIRNGISVYNVTRIKNTKME
                     FHIDRQDIKDFKNVYRGSKFDIKVKQKTGVPFIIKRVYKHKGMWICALVSLFLLMSTS
                     QFVTDVYIQSPEGIKKEALRNELYKVGVRPGVYKKSIDRKEVRDHMMSKFNDVAYLSI
                     NVKGTNIFVTVTKKAESLKSTDQSNYCNVIALKNGIIEKVIPRSGKSVIKSGDIVQKG
                     DVLLNGANTKSIPEVWASTFYESTKKASYVDTVNKKTGEKKNIYTLSFYDKEFTMRKN
                     IKYKDYVVENKEKKLSIGNYTFPIKIKTSTFYETKKVEVKRNKEELKKELSEKALKEL
                     EYIIPASARIIDVKHNFKVNKNMLEYLITVQTSENIAKIYPLSKSEAERFIKEESKPD
                     EGEEEVPSNPEKRPLNDIRNEFDEDNKDNKDQNNSDENNSNQNSNNSQNNNSNNN"
     gene            13277..14392
                     /locus_tag="KPD58_001056"
     CDS             13277..14392
                     /locus_tag="KPD58_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBG0264839.1"
                     /translation="MKIIVIKIVIIVKIIIAITTNKIKLKILGGLIQLIIQKKFTIAD
                     GNFERELFGNFDENVKLIEKTLNIDVILREGNIILIGEEKNVDSALKLMNELHQTVSN
                     GKHLDKQSISYSLSLLLEGSEQKIKELEGTIVITQRGKAVQPKTLGQKEYIKLIENND
                     ITFGVGPAGTGKTYLAVAMAVKAFKRDEVSRIILTRPAVEAGESLGFLPGDLKDKVDP
                     YLRPLYDALFEMLGADKFNKYLERGTIEVAPLAFMRGRTLDNSFIILDEAQNTTSEQM
                     KMFLTRLGFGSKAVVTGDVTQTDLPQNKKSGLIQATEILKGVPGIGSIMLTDRDVVRH
                     ELVQRIIRAYEKHDKREEFKKEERKKAKMQEKTFKRK"
     gene            14449..14910
                     /gene="ybeY"
                     /locus_tag="KPD58_001057"
     CDS             14449..14910
                     /gene="ybeY"
                     /locus_tag="KPD58_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBG0264840.1"
                     /translation="MDLILDDRQDKLEVSEELIEKIKDIIIECLDYEGYDDNYEVSLS
                     FVDNKEIHELNREYRGVDRVTDVLSFPLLSDDFEDVELEEESLGDIVVSLERALEQSI
                     EYNHSFEREVCFLICHSMFHLLGYDHDTDENTKEMREKEEHILNKLNITRE"
     gene            14916..15635
                     /locus_tag="KPD58_001058"
     CDS             14916..15635
                     /locus_tag="KPD58_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBG0264841.1"
                     /translation="MKPEKTRQGIIKAFNAAIEGILYTFKFERNMKIHYLGSVAVLII
                     SLFFNFSKLEMIMLLMSICLVVVAEMFNTAIEKAVDLVTDEYHVLAKIAKDVAAGGVL
                     VAALNSVVVGYILFYDKLTDISGILIYKIRESELHITLICILLVLIAVVVVKALTSTG
                     TPLKGGMPSGHAALAFAIATAITLMTERVVASTLAYIMAVLVAQSRIEGKIHTFWETI
                     AGALLGVLIAILVFQLGMFYN"
     gene            15754..16155
                     /locus_tag="KPD58_001059"
     CDS             15754..16155
                     /locus_tag="KPD58_001059"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBG0264842.1"
                     /translation="MDNIELLRLAEDARQHSYAPYSGFRVGAALLTKSGKVYTGCNIE
                     CASLGGTNCAERTAIFKAISEGDKDIYKIAIASDNSENNEQTYPCGICRQVIIEFGSD
                     IKIITGYTKGEVFEHSIKDLLPNYFSGNDLK"
     gene            16219..17112
                     /gene="era"
                     /locus_tag="KPD58_001060"
     CDS             16219..17112
                     /gene="era"
                     /locus_tag="KPD58_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBG0264843.1"
                     /translation="MFKSGFVSIVGRPNVGKSTLMNNVVGEKIAIMSDKPQTTRNTIQ
                     AVYTDEEMQIVFLDTPGIHKPKNKLGEFMVKAATEAFKNVDLILFVVDDSKKIGPGDR
                     KIIEDLRSVKTPIILVVNKIDQLDQKDELFDIIKMYDREGIFKEIVPISALKGKNTDT
                     LIKVIQNYLEEGPKYFPDYMITDQPERVLIAELIREKVLHYLNDEIPHGVAVEIEKMK
                     ARNDKEIVDVSAVIYCERDSHKGIIIGKNGRKLKGIGKSARQDIELLLGSQINLQLWV
                     KVKENWRNLQNYINNFGYNDK"
     gene            17124..18503
                     /gene="mgtE"
                     /locus_tag="KPD58_001061"
     CDS             17124..18503
                     /gene="mgtE"
                     /locus_tag="KPD58_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HBG0264844.1"
                     /translation="MDRKLDASQDLLYEVKSLIDNNKVLELRELIEEYHIIDIFDIME
                     NLEEDMKIQLFEVLPLDMASSILEEGSVEFFISILSKLDVEHSKNILELMSLGDMADK
                     LSELEEEEREHIINLLNQENADYVKELLFYDEDSAGGTMTTGYISINKDMTALEAIDH
                     MREEAEEAETIYYIYVVDDEEKLVGVLSLRELIIARDANIVEDLMSENIISVYVDEDR
                     EEAVRLVSKYNLIAIPVVDRQEKLKGIITVDDIIDVMEEEATEDMYKFAGSSEHEREV
                     AEKENPTLREQIISALRGRLPWLIITLVGGLLASLILSNLDYIMNPVYAPLVFFIPVV
                     IGMGGNIGTQSSSVTVITLSNKDLNFSNVVREGIVGIITGLLCSIITGIVIYFVMRKL
                     DIVLIVSISLFINMVLGATIGAFMPVLLKKMDADPSTVSSPIISTALDITGIAVYFII
                     TTALLSKIV"
     gene            18576..18731
                     /locus_tag="KPD58_001062"
     CDS             18576..18731
                     /locus_tag="KPD58_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HBG0264845.1"
                     /translation="MNNISWEVFKKTGSIDAYLYFCDCKNIHEEIEEVREKKEDDDNP
                     EHPGDST"
     gene            18697..19470
                     /gene="recO"
                     /locus_tag="KPD58_001063"
     CDS             18697..19470
                     /gene="recO"
                     /locus_tag="KPD58_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBG0264846.1"
                     /translation="MIILNTQGIVLKAIRYKESDIILTLFTRKLGKVSAIAKGAKKNK
                     SSLLSSSQLFSYSNFTLKKQGNMYKVTQSEIIKSFYNISYDIEAFSYATYITKLVENS
                     ILENQTNNRLFILLAQTLYLYTQDNTDNRFITAAFELKFLDYIGFKPIVNKCINCECK
                     ILQNSVFNIYEGGILCSKCSKLFDNNIKLDLTTISLMEYVLRNDILTCSKAKVSKYIT
                     HELENILDKYLKVYVDNINFKSLHVLQSVKNNKGVDNDE"
     gene            19463..20062
                     /locus_tag="KPD58_001064"
     CDS             19463..20062
                     /locus_tag="KPD58_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HBG0264847.1"
                     /translation="MSSNITIEMVDSVFERVPGATYAEAKEALVMCNGDVIEAVIYLE
                     NKKNTCNCKAKSAKETMEEVFGKDGENIKFQLKELLRKSSVVRIIVEKDGKTMMNIPL
                     TVGVVGLAFVPLATLVGLSAAVISKYRIKVQNEEDGEIVDLGELNEEKLHVLKNMITN
                     AAKDVKDVVVDKKEDDKDVTADLMKEESEKNKEDEDENK"
     gene            20222..21100
                     /gene="glyQ"
                     /locus_tag="KPD58_001065"
     CDS             20222..21100
                     /gene="glyQ"
                     /locus_tag="KPD58_001065"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBG0264848.1"
                     /translation="MNFQEMILALQKYWSKQGCIMMQPYDIEKGAGTMNPNTFLRSLG
                     PEPWQVCYVEPSRRPADGRYGENPNRLYQHHQFQVILKPSPDNIQELYLESLKEIGID
                     PSEHDIRFVEDNWEAATVGAWGLGWEVWLDGMEITQFTYFQQVGNIECELETGEITYG
                     LERLAMYIQEVDSVYDLKWNDKITYGEVFNKAEYENSMYAFELCDADMLFNLFDIYEK
                     EALRLMENGLVIPSYDYVLKCSHAFNTLDARGAIGVSQRASFIGRVRNMAKTVAETFV
                     KQREEMGFPLLKDGDK"
     gene            21100..23166
                     /locus_tag="KPD58_001066"
     CDS             21100..23166
                     /locus_tag="KPD58_001066"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBG0264849.1"
                     /translation="MKNYLLFEIGVEEMPSRFVGSTLEQLKNNLSKLFDENRIAFDKI
                     NAYGTPRRLTLVVEGLSDRQNDLEEEIKGPAKKIAVDAENNLTKPALGFIKSKGLSES
                     DIYFKQVGKEEYIFATIKQDGKETSEVLKDILPETIKSVVFPKAMRWGGKNLKFARPI
                     RWIVALLNDKVLEFDLEGIISSNVTKGHRFLGESTIEVDSIEDYFEKLEKNYIVLDQN
                     KRKEMIRKQCIEVANSLGGEVEFDEDLLEEVTYLVEYPTAFYGEFDVDYIKLPKEVVI
                     TPMKQHQRYFPVLKEGKLLPNFIAVRNGDSYRIDNVKAGNEKVLEARLADALFFYRED
                     TKRNLESYIEKLKTVVFQVKLGTIYDKTLRLEKLSADILDSLGLVDEKADTIRAAKLS
                     KADLVTGMVGEFDELQGFMGKEYAKVGGENDVVSEAIFEHYLPRFAGDILPKTNAGVA
                     LSIADKLDSIAGFFAIGIQPTGSQDPYALRRQALGILSILMDRKVAVDIKVLIEHALD
                     NYSELDFDKEEVVEQIMNFFNERVKNLFKDLGIRYDVVDAVLSSDINDVSDMHMRALE
                     LNNWLEKDELVEMLTAFNRVSTLAQKAESDKIDESLLKEDAEIKLYNEFKQVKIKVNE
                     LLKDKKYGIALDEFASLRPVVDNLFDTVMVMDNDEAIKNNRLALLKQVYDTMLEICDL
                     SKIVYK"
     gene            complement(23332..24879)
                     /locus_tag="KPD58_001067"
     CDS             complement(23332..24879)
                     /locus_tag="KPD58_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HBG0264850.1"
                     /translation="MDNFKKILLNELPNFKEYSSKFLNGEINKMQYKGFSGGYGVYAQ
                     RDKKSFMIRLRVSAGVLSQYQLNKIYEIAIKHNLDKIHLTTRQAIQLHDLSINSIVDI
                     MEEGIKNDIFTRGGGGNYPRNVGLSPLSGVDPSEVFDVTPYAVATDKYFIKNATTYHL
                     PRKLKVSYSNCYTDTAHCSIQDLGFVATLKNDEPYFRVFLGGGLGKQPKVALELDELI
                     KPKDALYCVEGMIKFFMDYGNYENKNRARVRYMVESLGEELFLEKFKEYYKLEKENGS
                     LELNIEEIDYSKPGVKIDIQDSRLIPQKQDGLYTVYIHPVGGILLTKDLSTLLKELDN
                     VENPMIRLGMTEGLYILNLNGNEAKRILEISKTISCNSQLEQSISCIGVPICQMGIQN
                     SQKMLHEIIDYFRLQNNEEILNKAPKLYISGCLNSCGVHQVGSIGLCGKKKNVDGIST
                     DTFELFVGGSFEIGKTRLGKSLGDFKASDIPEMLYKISDASSGNFYEWVNSNDNILNK
                     ITDKYKI"
BASE COUNT        10157 a         2489 c         4500 g         7766 t
ORIGIN      
        1 aaaatattta gtaatataga taatttaacg tatgtaaatt ttattgaaga attggataaa
       61 tcttctgcaa ctgctaaaca aaaggaagat attaaaaaag attttaaaaa caggaggttt
      121 ccatatgtta gaaatagtgg taagtatgga ttaaacacga gtttagaaaa tatggaaaca
      181 aatgtaaaaa gaatagtaga tactgtaatt aaatcaaata tagagataga tgaaatagat
      241 aaacttgatt tgacacaata tgaagaaaaa gatagtgtat gtaatgtttg caatataaat
      301 aaaacaacaa aactagatat tgatttaaga gaacgaaagg actctaagta taacttttta
      361 tttagaggtg cagaaaaatc aggctttaaa agaagtggac aagttgaaag taatatttgt
      421 tttgagtgtg aatttttcaa cttaatgtgt cttttatata taaatttaaa aagaccaatg
      481 gtttttgcat atacgaatga tttaagagaa ttagcatttt taaatcataa gataatgctt
      541 aaaagaaaaa tgtattcaga taaatcattt tataaaaaac ttttgcatga aaaaataagt
      601 tcaattagac tatatagatt tgatattgtt aagaataaag gtataattct aaagtttgac
      661 tctattatag agtataaaga attattaaaa aaaattgaac ttatagatat tatagataat
      721 tataatttct caagggagcc agtaaacact aaaaatttag gtaaaaaaat gattgataat
      781 ggaaatttat caaatttaaa agaattatta cttgataata taagtttttt aaagcaatca
      841 actggaactt ctcgagaaat ggattttgta tctagctcat ataatatcgg tttatatata
      901 aaattgtgtt ggattgtaga tggaggggag gagtataaaa tgaaaaacta tatggaaagt
      961 aatttaatat attcaagagt aggtaaagac ttatttaata aaatgacaga tgaatccaga
     1021 agaaaaaatt tttctatgaa agtgattcaa ctacttaagt ctaatgatag aactcagcta
     1081 tttcaaacta taatgcatgt gttagtttct aatggaatct taataggaga agggtttgta
     1141 gaaggtctaa tgcagagttc tgaattagaa ttaaatacga atgtaggtct atttattcaa
     1201 gaaatcatga aataaaaaag ggggaaaatt taaaatgaat gaaaaaagag taaaaggagt
     1261 aacattaact atactgacag aaagcccagt tccactttca tatgaccaag ggtatggaaa
     1321 ttatacacca ataaaaaaag aacaatatag agaaaaaata catgcaaaaa ctagtatagc
     1381 cacaataaca tatgatttaa gaagaatgct ccaccaagaa tatggttgga atttaagtaa
     1441 tattgttttt ggaaaaaaag gaaatatata tccatctatt aagaaaaatg taggatgtgt
     1501 agatgaaaat ggattagaga ctgatgtatt tggttatttg ataccactta aagataaagg
     1561 ttctataagt aaagcttctc cacttagaat aatacctttt aggtcattaa atccatatat
     1621 aggttcaaca cagctaataa caaatagagg ttttttaagt agcgaatttg gtagaaaata
     1681 ttatgatgag aaggaagaaa atgaagtacc tagagatgaa aactttccaa caacacaagc
     1741 attagcaata gaagagactt tatcagatta ctacgtttat acaataacat tggagcttga
     1801 tagaatagga gtagtagaag tagaagatgg gaagttactt ttaccagaag aaagaaaatt
     1861 tatgagtaag gaattaagag aaaaggcagt aaaagatata ttagatgcaa ttactgtgtt
     1921 tacaagaaac ataaaacatt catcagtact attaaaacca cttgcagtaa taggtggagc
     1981 ttttgataaa gttgtaccat ttttttggga tgatgtagat tataacgctg atagtggtga
     2041 gataaattta gagggagtca tagaaactat agaaagttat agccttaaag aaagtaacac
     2101 aatacttgct atcaatgaca gattaaaaat ttctaataaa aaggaattaa ataagtttaa
     2161 tttaagtaaa taccctgtaa aagagattaa aaatctagca gatagattgg aaattggaga
     2221 agataatatg tggtatttaa aagagtagga ggttttttga attatgaaag tattacaatg
     2281 taaacttaag caaccaactg ctcactatag agacccaaaa gtatttcaaa atgaatatat
     2341 aagtacttta aatttaccat ctaagaccac aataatggga atgataacgt atttatgtga
     2401 tagaagatta aactctgata tagatatagg aataattgga acacatcatc atagagaact
     2461 agaattttca agaggagaga acattgattt ttggaatgaa tatacaaata tgagaaaagg
     2521 caaagataaa gaaaagtttt tgcttcaagg aaactactat gattattata aagaacataa
     2581 agctcaaaat agcatattaa actatgaagt tcttaaagaa gtagaactga ctatatttat
     2641 ttcttgcaag gatgatgaag aacttgaatt tattaaaaaa aagcttgaaa gtccatgtaa
     2701 atatgcaaat ttaggaagaa aagaagattt tgtaatacca agtgaaaaag ggtgttttgt
     2761 gaaagaagtt gagttaaaag aggttatacc tatgaatact cgtgatgcta taaaagaaaa
     2821 tataaaatta aagaatacat atgtaagagt tgacttaaga gataaagaga atgtcgagac
     2881 tataataaat caaggtgtat tgattgcatt acctcataaa tataaagatt tagaagcaaa
     2941 tagagatgat agagtttatg agttttgtca ttatatatat gttgataatg atggtatata
     3001 tcctaaaaat attaaggtaa atgtatgtat tgatacaaaa gaagttttta catggcttta
     3061 aattattttg aaagataggt gtttatcatg aaaaatatca tgcaaaaaga aatattagct
     3121 aagtcataca atttaagaac taatcaacaa gagcaaacat tagttgaaca tattaaggat
     3181 ttatttgaag tattagaatc tatattagaa ctaaatctat attcagataa agatgtagag
     3241 atattgaaaa tatgttgtgc tttgcacgat ttaggaaaaa ttaattcaat tatgcaacaa
     3301 aaaatggaga ttaataataa aatatcgtat agttgctcag aagaagagag gaaaaagtta
     3361 gagtctgaca aaaaatcttt aaaaaagata gcaagacata atatattttc tggagcattt
     3421 ttaaaagata tattagaaaa aatgaacttg tcagaagaag ataagttata tatatataaa
     3481 agtataatgt tacatcatgg aaactatgaa gattatatga ggttaagtat tagtaaagtt
     3541 caagaagaaa tttatgagta tattgaaaaa ggcattttag aaaaagaaga gtttaatctg
     3601 aaagatatag aaagttatat aaataacatt ttaaatgtag actttaaatt tggtgaagat
     3661 gtattggatt atgattacat agataaatta agtgaaagta tgtttataga cagtgattat
     3721 aacaatggtc aaatagatga ttcagtttta aattatagaa agtttaagta tattttatat
     3781 aaagggatgc tgaacttaat agaccatagt gcatcaagta gacagaaagg tataaaattt
     3841 tataacgatt ttactgatga agaaattgat aatatgattt taaatgagat atataagagt
     3901 cagggtaatt tagaaaaaaa taatatagag ttcaatacta tacaaaaaag gttgagagaa
     3961 ctttctggaa gaaatgttct gactgttgca tttacaggtt caggaaaaac agctgcagat
     4021 tatagaaaga cttttaaaag aaaattcttt ttagtgccaa ataaaatatc agcagaaagt
     4081 ttttatagaa aaaatatttt tcaaaataaa aatgatttag atacaagaag tagtaatgat
     4141 tacattggat tacttcatgg agatataaac ttatattcag agaatgaaga taatggagaa
     4201 catgattttg tattgacatt aagggatata gatttgagta taaacttttg taaaccttgt
     4261 gtaattgcaa cagttgacca gttattactt agtatgttta aatttccggg atatgaaaaa
     4321 atattcgcag ccgtaaaaaa tgcatctata actgttgatg aagtacattt attggaacca
     4381 aagatgtttt tgattttgat atattttatg cagtttgcat gcaagtactt agatgttgat
     4441 tttcacttaa tgacagctac acttccaaaa tcatataaag agcaaatgat aaacaaggga
     4501 attatttttc aagaagaaag taataaaaat gttactgata aaggagaaat agtttttatt
     4561 gagagcaaca aggaagaaga gatttgtgaa ggtaaagatg taaaggtttc ttttataaag
     4621 gaaaaagaaa tcaagtcaat agttgaagat gctttagaaa ataaacaaaa aattcttata
     4681 ataaagaata ctattgattc agtcaataga acatatgagt ttttaaaaga aaatttatct
     4741 gataagtatg gtggtgtaga tatagatgtt ttacatagta gatttaagtt caaagataaa
     4801 aaagagaaat atagtaaaat attaaatgga gagggtgata tttggatatc aactcaatca
     4861 gtagaaatat ccttggattt ggatttcaat ataataataa gtgatttagc aactatggat
     4921 agcttgatac aaagaatggg tagatgcaat agaaataata aatatgaata tggaaacttt
     4981 tatattctac ctagtgaaga taaaatttat gataacaaac ttaagaacac aactaaaaac
     5041 atcttaaagg acatattaaa aactaactct atttttacaa tgggaattag aaaaactatt
     5101 ttggatgatt actatgataa tagtgtagtc aaaaaatatt ttgaagagaa ttttataagt
     5161 tgtgataaag aaattaagaa tatttatggt ataaataaag aaatttttga tggattagat
     5221 ttaattttta attttgaacc ttacaaaaat attgttgata gtaagaagga agcagctaaa
     5281 atttttagag atattgatgt tagttacaaa attattctgg aagaagattt ttataaggaa
     5341 gatagggagt tacaacaaga ttcgattcaa gtatcagggt ttatatttaa tagattatat
     5401 tattatggat taataagtaa agtagaagga tacatggtac taaaatctag tagtaagttt
     5461 gagtataata gtactgtagg cttaattttg aagtgagcat atgtttaaat ataatatacc
     5521 tttagccatt gaaaatacaa aaagtactac tgtaagtcca ctgtaaaaac tattttttaa
     5581 aaggaaaaat aagttgtgag atagagtgtt tacaagtgta tatagataat aaatttgagg
     5641 tttaagatta actaagtggg tttcaggtac atcgcgaaaa taattataag ttggagattt
     5701 tgtatgttta agattaacta agtggatttc aaatattgat atagtagata taatagaagt
     5761 tttattatta ggtttaaaat taactaatcg gatttttaaa tattctgact attatataag
     5821 gatattatta gcttgaattt aaaaccaata atataaaaca acaaaatatt atatttatga
     5881 tataataaaa gttatggaaa aacaggaata ttactataga gaagagaggt aaacttatga
     5941 ataattggat agaagtaact ataaaaacaa caacagaagc agttgaacct ataacgaata
     6001 tactatatga acaaggagct ggaggagctg taatagaaga cccaaaagac tttttattcc
     6061 aaaagaaaaa tgaactagac tgggattatg ttgaagaaga agtatttaaa aagaatgaag
     6121 aagatgatgt tcttataaaa acttatgttt ctgaagaaaa aaatgtgatg gaatttgtcg
     6181 aaataataaa gcaaaaagtt ttaggattaa aagattttgg tatagatata ggtgaaggaa
     6241 gtgtatcatt ggaccaagta aatgaagctg attgggcaaa tgcatggaaa gcatactaca
     6301 aaccaacaaa agtaggacaa agggtagtag taaaacctac atgggaagat tatgctatgc
     6361 aagaggggga tttaatcata gagcttgacc caggaatggc atttggtaca gggacacatg
     6421 agactactag tatgtgcatt agagaattag aaaagtatgt gaataaagat tctaaagtat
     6481 ttgatattgg ctgtggtagt ggaatccttg caatagcagc agctaaatta ggagcaaaag
     6541 aagttgtagc tgtagattta gatgaagtgg ctgtaaaagt tgcaaaagaa aatgttcttg
     6601 aaaataaagt tgaaaaaagt gtatctgtaa tgcatggtaa tttaactgat gtaataaaag
     6661 ataaagcgga tgtaatagtt gcaaatataa tagctgatat aataaagata cttgcaaaag
     6721 atgttcaaaa ctttatgaaa gaagatgcta tattcatatc ttcaggtata attttggata
     6781 aagtagaaga agttaaagaa agcttaattg aaaatggatt tgaaatagtt gaagttcaaa
     6841 aattaggtga atggtcagca atagtttcta agcttaaaaa ataagggata tggtgacgta
     6901 taatggatag attttttgtt gaaaaaaata atataaatct acaagataaa acttgcacta
     6961 tagaaggtga agatgtaaaa catatttcaa aagttttaag atgcaaatta ggtgagaaac
     7021 ttgaaatatg tgacaagaat aataatgagt atatttgtga gataatgaat atagataaga
     7081 gtatagttaa tttagaaatt ttagaaaaag ttgatataaa tagagaatca gaacttaaag
     7141 taaggctata ccagggactt ccaaaagctc caaaaatgga aatgatactt caaaaattaa
     7201 ctgaagtggg tgtagaagaa ataatattag ttcaaactaa gagaagtgtt gttaaagttg
     7261 atgacaaaaa agaagataaa aaatttgaac gttgggaaag aataatttat gaagcagcaa
     7321 agcaaagcaa aagaggtaag ataccaaaat taagaggtgt tttaagtttt aaagaagctt
     7381 tagaagatat gaaaaaaaat aatgtaaata tttgccctta tgaaaatgaa agaacagttt
     7441 caataaaaca tgcactaaaa aaatgtgatt ctaatataga tagtgttgga atttttatag
     7501 gtccagaagg tggattttct gaagaagaaa tagaacaaat acaaaaaaat aattgtaatg
     7561 ttgtatcatt ggggcctaga atattaagaa ctgagacagc ttctgttgta gcatcaacaa
     7621 tagctttgta tgaactaagt gatttaggag gagaaaaata aattgaaaaa agtagctttt
     7681 tatacacttg ggtgtaaagt taatcaatat gaaacagaag ctatgctaga actatttgag
     7741 aaagatggtt atgaacaagt caatagtgaa gagtatgctg atgtttatgt tataaataca
     7801 tgtacagtta ctcatatgag tgatagaaaa tctcgtcaat atataagacg agttaaaaag
     7861 aagaatccag atgcaataat agccgttgta ggatgttatt ctcaggtatc tccggaagaa
     7921 attttggata tagaagaagt aaatttagtc atgggtacaa atgatagaag aaagattgtt
     7981 gaagaaatta aaaagataaa ttcaagtaag aaagttagta cagttgacga cataatgaaa
     8041 gttaaagctt ttgaagaaat agaaatcagc caaactaatg gaaagactag agcatttatg
     8101 aaaatacaag atggatgtga cagattttgt acttattgta tcattcctta tgcccgagga
     8161 cgcgttagaa gtagagatat tgatagtata gtcgatgaag tgaaaaaatt ggctaataat
     8221 ggctataaag aggttgtatt aacaggaata catgttgcct cttatggaaa agatttaaag
     8281 gatagggata ttaaacttct tgatgtaata aaacaaataa accagataga aaaaatagaa
     8341 agaattaggc ttagttctgt tgaaccaatt ttatttacag atgaatttgt aaatgaagtt
     8401 ttaaagatgg ataaagtttg tcctcattac catttatcac ttcaaagtgg atgtgatgaa
     8461 actctaaaaa gaatgaatag acgatacaca actttagagt acaaaactat tgtagataga
     8521 ttgagaagta aaatgccaga tgtggcaata actacagatg taatagtagg ttttcctgga
     8581 gagacaaatg aagagtttaa aaaaacgtac gagtttttaa aagaaataga gctttcacaa
     8641 atgcatattt ttaaatattc accaagaaaa ggaactccag ctgcaactat ggaaaatcaa
     8701 gttgaccctc aaatgaaaca ctttagaagt gaacaacttt taaatttaag taaagtaaat
     8761 tttaataaat ttgcaactaa atttatcggc cgtgagttgg atgttttgtt tgaacaaaat
     8821 atagaaggca ataaatatga gggattaact tcaaattata taagagttgt tgttgaaagt
     8881 gacaaaaata tacaaggtca gatattaaaa gtgaaaataa atgatgtaaa agatgaatat
     8941 gtagaaggaa ttttgctata acatgtagaa attataatat ttataagata ggaggtggaa
     9001 agaatggatt gtattttttg taaaatagcg aacggagaaa taccttcaac taaagtttat
     9061 gaagatgata gagtcttagc ttttaatgac ttgaacccag ttgctccata tcatatatta
     9121 gttgtaccta aaaaacatta tgatagttta attgatattc ctgataaaga gatggacatt
     9181 gtgtcacata ttcatgttgt tattaataaa atagctaaag agaaaggttt tgaccaaact
     9241 ggattcagag tcataaataa ttgtggaagt gatggagggc aagaagttaa acatttgcac
     9301 tatcatattt tagctggtaa aaagcttcct aattacgaag cgggacaaaa ctaaactaaa
     9361 ttattgaaaa attgagttcc ttcttgataa aaatacttga taaaagtaaa aatttagttt
     9421 ataataaaca aagtgttagg ttgtaatgca tattatgcaa aaagaacata gttacagtcc
     9481 cagcataatt gcgctattgg agggagggaa aaagaaatgt cagaagtaag agttagagag
     9541 aatgaaacat tagacagcgc tttaagaaga ttcaagcgtc aatgtgcaat gtctggcatc
     9601 atgtcagaag tgagaaaaag agagcactat gataagccaa gcgttaagcg taagaaaaaa
     9661 gctgaagcag caagaagaaa aaacgctaag aaatagtagt aaataagaat aaagaggtga
     9721 aggggatgtc ccttaagcaa aagttacaag aggatttgaa atcttcgatg aaaaacaaag
     9781 atacagtgag aaaatctgta gtaactttaa taagagcttc tataaagcag tatgaggttg
     9841 ataatagggt cgagcttgat gaagatggaa ttatcgatgt aatagcaaaa caattaaagc
     9901 aacgtagaga tgcattagtt gaatttgaaa aagctggaag agaagactta ataaaagaaa
     9961 cagaaggtga aattgaagtt ttaaaagagt acctacctca acaattaagc gaagaagagt
    10021 tagaagaaat tgtaaaatct actatatctg aagtaggggc aacttctatg aaggatatgg
    10081 gaaaaataat gtcagttatc caaccaaaag ttaaaggaag agctgacgga aaacttataa
    10141 ataagttggt aaaacaaaac ttacaataga ttgatatagc ctagagagaa atctctaggc
    10201 tttttatagt ttttgggaat aacgagtcat atttgagcat atctataatc aaagaaaatt
    10261 ttgaaagaag gggaaaaatt tgattatatc agtaaaagag attaggaatt ttttgataaa
    10321 catatttata ccactaataa taggttactt aagtagcatc ttaacaatga ttatcagtgg
    10381 aacagatatt tcaacctatt atttacaact cacaaagcca agctttgcac caccagcttt
    10441 tatctttcct atagtttgga ctatattgta catattgatg ggtatatctt catacctaat
    10501 attaagaaaa ggttacaact tacctaaagt aagagatgca attttttatt atggattaca
    10561 acttgtatta aactttattt ggagtatact attttttgga tttggactta gatttacagc
    10621 tttgatagat ataataataa tgattttggt ttctataata atgatttcga agttttctaa
    10681 aatagataag agatctggat acataaattt gatttattta atgtggcttg tttatgcagg
    10741 atttttgaac tattttatat ggtttattaa taaatagaat gtaaagattt tgtaaattac
    10801 cctgtacttt taataatatc ataacaaacg aaaaataaat agactattaa aaataatcat
    10861 tttcatatac aataatatta atttaaatac aataaattta taaaaattgt atttaaagat
    10921 taacatatta aagtattaga attggaaagg cttgaaggga gaggttttta tgcaaataat
    10981 agtatctggg agacaaatga aattaactga cggaataaaa ggatacgtag atggaaaatt
    11041 aagtaggtta gaaaaatacc ttgatcctga atcagaagtg aaagtaacag taagtgcgaa
    11101 aaaggataga caaaaagtgg aggttacaat aattcctatt aacggtcaaa taataagagc
    11161 agaagatgta gaagatgact tatatgcagc aattgatatt gtttgtgaca aattaagtag
    11221 acaagttgtc aaatataaaa ctaaagttaa agacaaagtt caaaataata agagcataag
    11281 atttgaaaat cttgacttta tagataatag cagtgaattt gatgattatg attatgatga
    11341 tgaagaagat gaaaatatag taatagaaag aagaaagaaa tttaatgtaa aacctatgag
    11401 ttctgaggag gcgatacttc aaatggaact agtaggacac aatttctata tgtttagaaa
    11461 ccaagataat tttgaaataa acatagtgta taagcgtaaa gctggtggat atggtttaat
    11521 agaacaagat taataaaaat tgtatacgtt tagattttat ataagaaaaa ataaaaaggt
    11581 aatcatgttg attttttatc aatatgatta cctttttctt gtttaaataa tatattagat
    11641 attaggtatt tattatataa aaattttaaa actaatctgt tacaacatca ataaagtgca
    11701 taaataccta tttcaagcat aaaattaatt aagggggtaa tgcttatgtt agaaatatcc
    11761 actgatttac aagtaaatca accagttatt actgttacaa gtaatacgtt tattagtata
    11821 gaaaattatc tatcaatatt agaatatgaa gttgatttaa taagaataaa gactaaagtt
    11881 aaaacaatta aaatatctgg ggataaactt tcacttaagt atattactga tagtgagata
    11941 ggtataaaag ggattatcta caatgttgag tatgttgatt aggaggagat acacttgata
    12001 agttttataa ggggatacta tgttatagtt gtggaaggag taggactcga acaattttta
    12061 aatcatctta taagaaatgg gataagtgtt tacaatgtaa ctagaataaa aaatactaaa
    12121 atggagtttc atatagatag acaagatata aaagacttta agaatgttta tagaggaagt
    12181 aagtttgata taaaggtaaa gcaaaaaact ggggtccctt ttattatcaa aagagtttat
    12241 aagcacaaag gtatgtggat ttgtgctctt gtttctttat ttttattgat gtctacatca
    12301 cagtttgtga cagatgtata tatacaatct cctgaaggta taaaaaaaga agctcttaga
    12361 aatgagcttt ataaggttgg agtaagacca ggagtctata agaagagtat tgatagaaaa
    12421 gaagttagag accatatgat gtcaaaattt aatgatgtgg catatttatc tatcaatgta
    12481 aaaggaacaa atatatttgt tactgttaca aaaaaggcag aatccctaaa atctacagac
    12541 caatcaaatt attgtaatgt aattgcttta aaaaatggta taatagaaaa ggtaatccct
    12601 agaagtggta aatcagttat taaatctgga gacatagtcc aaaagggaga tgtcttatta
    12661 aatggagcta ataccaagtc aataccagaa gtatgggcaa gtacttttta tgagtccaca
    12721 aaaaaggcaa gttatgtaga tactgtaaat aaaaaaactg gagaaaagaa aaatatatat
    12781 acactaagtt tttatgataa agagtttaca atgagaaaaa atataaaata taaagattat
    12841 gttgttgaaa ataaagaaaa aaaactatca ataggaaact atacttttcc aataaagata
    12901 aaaacgagta ctttttatga aacaaaaaag gttgaagtaa aaaggaataa agaagagtta
    12961 aaaaaagaat tatctgaaaa agctttaaaa gagttagaat acataatccc agcttcggct
    13021 agaataatag atgtaaaaca taactttaag gttaataaaa atatgttaga atatttaata
    13081 acagttcaaa ctagtgaaaa tattgctaaa atttatcctc ttagtaagtc agaagcagaa
    13141 aggtttataa aggaagaaag taaaccagat gaaggtgaag aggaagttcc ttccaatcca
    13201 gaaaaaagac cattaaatga tataagaaat gagtttgatg aggataataa ggataataaa
    13261 gatcaaaaca attctgatga aaataatagt aatcaaaata gtaataatag tcaaaataat
    13321 aatagcaata acaactaata aaattaaact taaaatctta ggaggactaa ttcaattgat
    13381 aatacaaaag aaatttacaa tagcagatgg taactttgaa agagagttat ttggcaactt
    13441 tgatgaaaat gtaaaattaa tagagaagac tttaaatata gatgtaatac ttagagaagg
    13501 caatataatt ttaattggag aagaaaaaaa tgtagattct gcattaaaac ttatgaatga
    13561 attacaccaa actgtatcaa atggtaagca tttagataag cagagtattt cttattcatt
    13621 atctcttttg cttgaaggaa gtgaacaaaa aataaaagaa cttgagggga caatcgtcat
    13681 aactcaaagg ggaaaagctg ttcaacctaa aactttaggt caaaaagaat atataaaact
    13741 tatagaaaat aatgatataa cttttggtgt tggacctgct ggaacaggaa agacttatct
    13801 tgcggttgct atggcagtaa aagcttttaa aagagatgaa gttagtagaa ttatacttac
    13861 aagaccagct gtggaagcag gagagagtct aggtttttta ccaggagatt taaaggacaa
    13921 agttgaccca tatttaagac cactatatga tgccttattt gaaatgttgg gtgctgataa
    13981 atttaataag tatttagaaa gaggtactat tgaggttgca ccacttgcat ttatgagagg
    14041 tagaacactt gataattcat ttataatatt ggatgaagct caaaatacta cttctgaaca
    14101 aatgaaaatg tttttaacta gacttggatt tggctctaaa gctgtagtta caggagatgt
    14161 aactcaaaca gatttaccac aaaataagaa gagtggtcta atacaagcta cagagattct
    14221 taaaggtgtt ccaggtattg ggtctataat gctaacagat agagatgttg taagacatga
    14281 gttggtacaa agaataataa gagcttatga gaagcacgac aaaagagaag aatttaaaaa
    14341 agaagaacgt aaaaaagcga aaatgcagga aaagactttc aaaagaaagt aaaagtgtag
    14401 taatatagat atatagcatt attaatgaat aaaaagtagg tgtagtgaat ggatttgata
    14461 ctagatgata gacaagataa attagaagta agtgaggagt taattgaaaa gataaaggat
    14521 ataatcatag aatgtttaga ttatgaagga tatgatgata attatgaagt gagtctttct
    14581 tttgtagata ataaagagat tcatgaatta aatagagaat atagaggagt tgatagagtg
    14641 actgatgttt tatcatttcc tcttttaagt gatgattttg aggatgttga gttagaagaa
    14701 gaatccctag gtgacatagt agtatctctt gaaagagcct tagaacagag tatagagtat
    14761 aaccatagct ttgaaagaga agtatgtttt ttaatatgtc atagtatgtt tcatctttta
    14821 ggttatgacc acgatacaga tgaaaataca aaagaaatga gagaaaaaga agaacacata
    14881 ttaaataagt taaacataac aagggagtag tttttatgaa accagaaaaa actcgtcaag
    14941 gtataattaa agcatttaat gcagctatag aagggatatt gtatacattt aaatttgaaa
    15001 gaaatatgaa aatacattat ttaggttcag ttgctgttct tataataagt ttgtttttta
    15061 acttttcaaa attagaaatg ataatgttac ttatgtccat atgcttagtt gtagttgctg
    15121 aaatgtttaa tacagctata gaaaaagctg ttgatttagt aactgatgaa tatcatgttc
    15181 ttgctaagat agcaaaagat gttgcagctg gaggcgttct agtggcagca ctaaattcag
    15241 tagtagttgg atatattttg ttttatgata aattgacaga tatatctgga atattaattt
    15301 ataaaataag agaatcagaa ttacatataa ccttgatatg tatattattg gttcttatag
    15361 ctgttgtagt tgtaaaagca ttgacttcta caggtactcc tctaaaagga ggaatgccaa
    15421 gtggtcatgc tgccttagca tttgctatag caactgcaat tacacttatg actgaaagag
    15481 ttgttgcctc tactcttgca tatataatgg ctgtattagt tgcccaaagt agaattgaag
    15541 gaaaaataca taccttttgg gagactattg ctggagcttt gttaggtgtg ttgatagcta
    15601 tattagtatt tcagcttgga atgttctata attaaaataa atgtacataa aatatttaaa
    15661 ataaaacaaa tctgtgctaa ttaaattttt atgatataat gtagagtatt aaataaaaat
    15721 aacttttaat tattgtatac aggaggagtt gttgtggata acatagaatt gttaaggttg
    15781 gcagaagatg ccagacaaca ttcttatgct ccttactcag gttttagagt aggtgcagct
    15841 cttttaacta agagtggtaa agtttatact ggatgtaata tagaatgtgc atcattaggt
    15901 ggaacaaatt gtgcagaaag aacagctatt tttaaagcaa tatcagaggg tgataaggat
    15961 atctataaaa tagctatagc aagtgataac tcagaaaata atgagcaaac atatccatgt
    16021 ggaatatgca ggcaggtaat tatagagttt ggttctgata taaaaataat aacgggatat
    16081 actaaaggag aggtgtttga gcatagtata aaggatttgt tgccaaacta tttctctgga
    16141 aatgatttaa aataaattta aaagtataaa taaatttaag taattaaaac aagaagataa
    16201 aaagttagga gacagttaat gttcaaatca ggttttgtca gtatagtggg tagaccaaat
    16261 gtaggaaaat ctactttaat gaataatgta gttggagaaa aaatagctat aatgagtgat
    16321 aagcctcaaa ctacaagaaa tacaatacaa gcagtatata cagatgaaga aatgcagata
    16381 gtattcttag atacaccagg tattcacaaa ccaaaaaata aattaggtga atttatggta
    16441 aaggcagcaa cagaagcttt taaaaatgta gatttaatat tatttgtagt tgatgattct
    16501 aaaaaaatag gtcctggaga tagaaagata atagaggatt taagaagtgt taagacacca
    16561 ataattttag ttgtaaacaa aatagaccaa ctagaccaaa aagatgagct atttgatata
    16621 atcaaaatgt atgatagaga aggcattttt aaagaaatag taccaatatc agcattaaaa
    16681 ggaaagaata cggatacctt aataaaagtt atacaaaatt atttagaaga agggccaaag
    16741 tattttccag attatatgat tacagaccag ccagaaagag tattaatagc agaacttata
    16801 agagaaaaag ttcttcacta tcttaatgat gaaatacctc atggtgtagc tgttgaaatt
    16861 gaaaaaatga aagcgagaaa tgataaggaa atagtagatg tatctgctgt aatatactgt
    16921 gaaagagatt cacataaggg aataataata ggaaaaaatg gtaggaaact aaaaggaata
    16981 ggtaagtcag caagacaaga tatagaattg cttttaggtt cacaaataaa tcttcaatta
    17041 tgggttaaag tcaaagaaaa ttggagaaat ttacaaaatt atataaataa ttttggatat
    17101 aatgataaat aaaggtgggt tatatggata ggaaactgga tgcttcccaa gatttattat
    17161 atgaagtaaa atcattaatt gataataata aagtattgga gttaagagaa ttaatagagg
    17221 aatatcatat aattgatata ttcgatataa tggaaaattt agaggaagac atgaaaattc
    17281 aattatttga agtacttcct ttagatatgg catcttctat attagaagaa ggtagtgtag
    17341 agttttttat tagtatttta tctaaactgg atgtagaaca ctcgaaaaat atacttgaac
    17401 ttatgtctct tggagatatg gcagacaagt tgagtgaact tgaagaagaa gaaagagaac
    17461 acataataaa tcttttgaat caagagaatg ccgattatgt aaaagagtta ctattctatg
    17521 atgaagattc tgctggtgga acaatgacca ctggatatat ttctataaat aaagatatga
    17581 cagctcttga ggctatagac catatgagag aagaagctga agaagctgaa actatttatt
    17641 atatatatgt ggttgatgat gaagaaaaac tagttggagt attgtcatta agagaattaa
    17701 taatagctag agacgcaaat atagtagaag atttgatgag tgaaaatata atctctgttt
    17761 atgtagatga agatagagaa gaagcagtaa gattagtatc taaatacaat ttaatagcaa
    17821 ttccagtagt agacagacaa gaaaaattaa aaggaataat aacagtagat gatataattg
    17881 atgtaatgga agaagaagca actgaagata tgtataaatt tgcaggaagt tcagaacatg
    17941 aaagagaagt tgctgaaaaa gaaaacccta cattgagaga gcaaataata tcagcactta
    18001 gaggacgatt accttggtta attattacct tagttggagg gttgttggct tcgttaatac
    18061 tttctaactt agactatata atgaatcctg tatatgctcc attagtattt tttatacctg
    18121 tagtaattgg tatgggtgga aatattggaa ctcaatcatc atctgttaca gtaataacat
    18181 tatctaataa ggatttaaat tttagcaatg tggttagaga aggtattgta ggtattatta
    18241 caggtctttt gtgtagtata ataacaggta ttgtaattta ctttgttatg agaaagttag
    18301 acattgtatt aattgtttca atatcattat ttataaatat ggttttaggt gcaacaatag
    18361 gtgcgtttat gcctgtttta ttaaaaaaaa tggatgcaga cccatctaca gtttcatcac
    18421 caattatatc tacagctctt gatataactg gtatagcagt ttactttata ataacaacgg
    18481 cattattgtc aaaaattgtt taataacttt atgtatgatt tatcctcttc ttactaaaaa
    18541 tataagtata aatgtaagaa gggggttttt taaaaatgaa caatatcagt tgggaagttt
    18601 ttaaaaaaac aggtagtata gatgcatatt tatatttctg tgattgcaaa aacatacatg
    18661 aagaaattga agaagtaagg gagaaaaaag aagacgatga taatcctgaa cacccagggg
    18721 atagtactta aagctataag gtataaggaa agtgatataa tacttacact atttacaaga
    18781 aagcttggaa aagtatctgc aatagcaaaa ggagctaaaa aaaataagag ctctttgctt
    18841 tcatcatcac agttattttc ttatagcaat tttaccttaa aaaaacaagg taatatgtac
    18901 aaggttactc aaagtgaaat aataaaaagt ttttataata tatcctatga tatagaggca
    18961 ttttcttatg ctacatatat tacaaaatta gttgaaaatt ctatacttga aaatcagact
    19021 aataataggc tatttatatt acttgcgcag actctgtatt tgtatacaca ggataataca
    19081 gacaataggt ttataacagc agcattcgag ttaaaatttt tagactatat aggatttaaa
    19141 cctatagtaa ataaatgcat taattgtgaa tgtaaaattt tacaaaattc tgtgtttaat
    19201 atctacgaag gtggtatact atgtagtaag tgtagtaaac tttttgataa taatataaaa
    19261 ttagacttaa caactataag tcttatggaa tatgtattaa gaaatgatat tttaacatgt
    19321 agtaaagcta aagtatcaaa atatataaca catgaacttg agaatattct agacaagtat
    19381 ttgaaagttt atgttgataa tattaatttt aaatcattac acgtattgca aagtgtaaaa
    19441 aataataagg gagtggataa tgatgagtag taatataact attgaaatgg ttgattcagt
    19501 ctttgaaaga gttccaggag caacttatgc agaagcaaaa gaagcattag taatgtgcaa
    19561 tggagatgta attgaggcag taatatattt agaaaataaa aaaaatactt gtaattgtaa
    19621 agcaaaatct gctaaagaaa ctatggaaga agtatttggc aaagatggag aaaatataaa
    19681 gtttcaatta aaagagcttc ttagaaaaag tagtgtagtg agaataatag ttgaaaaaga
    19741 tggaaaaact atgatgaata ttcctttaac agttggagtt gttggattag cttttgtacc
    19801 attagctact ttagttggat tatcagctgc tgttataagt aaatatcgta taaaagttca
    19861 aaatgaagaa gatggagaaa ttgtagattt aggtgaattg aatgaagaaa aacttcacgt
    19921 tttgaagaat atgataacta atgcagcaaa agatgtgaag gatgtagtag ttgacaaaaa
    19981 agaagatgac aaagatgtaa ctgctgacct tatgaaagaa gaatctgaaa agaataaaga
    20041 agacgaagac gaaaataaat agatataaag tgacttttaa aaaatgaaga gaagataatt
    20101 ataagaatta tcactaacat tgacaaattt tatagaattt gatattctaa aagttgtaga
    20161 agaactatta aaatagcctt tcgtgtagac gagaggcttt attgttatag gagggatttt
    20221 tatgaatttt caagagatga tactggcgtt gcaaaaatac tggtcaaagc aaggttgtat
    20281 catgatgcag ccttatgata ttgaaaaagg tgcaggaact atgaatccta atactttttt
    20341 aagatcatta ggacctgagc cttggcaagt atgctatgta gagccttcaa gaagaccagc
    20401 agatggaaga tatggtgaaa acccaaacag actatatcaa catcatcaat tccaagttat
    20461 actaaaacct tctccagata atatacaaga attatatttg gaaagtttaa aagagattgg
    20521 aattgaccca agtgagcatg acataagatt tgttgaagat aactgggaag ctgctacagt
    20581 tggtgcttgg ggacttggat gggaagtttg gctagatggt atggaaataa ctcaatttac
    20641 gtattttcaa caagtaggta atattgaatg tgagcttgag acaggagaaa ttacatatgg
    20701 attagaaaga ttagctatgt acattcaaga agttgatagt gtttatgatt taaaatggaa
    20761 tgataaaata acttatggtg aagtatttaa taaagctgag tatgaaaact caatgtacgc
    20821 atttgaatta tgtgatgcag atatgttgtt taatctgttt gatatatatg aaaaagaagc
    20881 attaagactt atggaaaatg gccttgtaat accttcatat gactatgtgt taaaatgctc
    20941 tcatgcattt aatacattgg atgcaagagg tgcaatagga gttagtcaaa gagcttcatt
    21001 tataggtaga gttagaaata tggcaaagac tgttgcagaa acttttgtaa aacaaagaga
    21061 agaaatggga ttccctcttt taaaggacgg tgacaaataa tgaaaaatta tcttttattt
    21121 gaaatcggtg ttgaagaaat gccatcaaga tttgtaggca gtacactaga acaattaaaa
    21181 aacaatctta gtaaattatt tgatgaaaat agaatagctt ttgataaaat aaatgcgtat
    21241 gggacaccaa gaagacttac tttagtagtc gaaggattga gtgatagaca aaatgattta
    21301 gaagaggaaa taaagggtcc tgctaagaaa attgctgttg atgcagaaaa taatttaaca
    21361 aaaccagcat taggatttat aaaaagcaaa ggattaagcg aatctgacat atattttaaa
    21421 caagttggaa aagaagaata tatatttgcg actataaagc aagatggaaa agaaacttca
    21481 gaagtactaa aagacatatt accagagact ataaaatctg tagtattccc aaaagctatg
    21541 cgttggggag gaaaaaatct taaatttgca agacctataa gatggatagt tgcattgtta
    21601 aatgataaag tattagagtt tgatttagaa ggaataatat cttctaatgt aacaaaaggt
    21661 catagatttt taggtgaaag tacaatagaa gttgattcta tagaagatta ttttgaaaaa
    21721 ttagaaaaaa attatatagt tttagaccaa aacaagagaa aagaaatgat aagaaaacaa
    21781 tgtatagaag tagcaaattc tttaggtgga gaagttgaat ttgatgaaga tttacttgaa
    21841 gaggtaactt atttagttga atatccaact gctttttatg gagaatttga tgtggattat
    21901 ataaaactac caaaagaagt tgttataact cctatgaaac aacatcaaag atattttcca
    21961 gtacttaaag aaggaaaact gttacctaac tttatagctg ttagaaatgg tgattcttac
    22021 agaatagaca atgttaaagc tggaaatgaa aaagttttag aagcaagact tgcagatgct
    22081 ttatttttct atagagaaga tactaagaga aacttagaaa gctacataga aaaattaaaa
    22141 actgttgtat tccaagtaaa actaggaact atttacgaca aaactttaag acttgaaaaa
    22201 ttaagtgctg atatacttga tagtttaggt ttagtagatg aaaaggcaga tactataagg
    22261 gctgctaaat tatctaaggc agatttagtt actggaatgg taggagaatt tgatgaatta
    22321 caaggtttta tgggaaaaga atacgcaaaa gttggaggag agaatgatgt tgtaagtgaa
    22381 gctatttttg aacattatct tccaagattt gcaggagata tacttcctaa gactaatgca
    22441 ggagttgcct tatcaatagc tgataagttg gattcaatag caggtttctt tgctatagga
    22501 atacaaccaa caggttctca agacccatat gctttaagaa gacaagcttt aggtatactt
    22561 agtatactta tggacagaaa agttgctgtg gatataaaag tacttattga acatgcttta
    22621 gacaattatt ctgaattaga ttttgataaa gaagaagttg ttgagcaaat aatgaatttc
    22681 ttcaatgaga gagtaaaaaa tctatttaaa gatttaggaa taagatatga tgttgttgat
    22741 gctgttttaa gttctgatat aaatgatgtt tctgatatgc atatgagagc tttagagctt
    22801 aataattggc tagaaaaaga tgaattagtg gagatgctta ctgcatttaa tagagtttct
    22861 actctagctc aaaaagcaga aagtgataaa attgatgaat ctttattaaa agaagatgct
    22921 gaaattaaac tttacaatga atttaaacaa gtaaagataa aagttaatga attattaaag
    22981 gataagaaat atggtattgc tttagatgaa tttgcatctt taagaccagt agttgataat
    23041 ttatttgata cagttatggt tatggataat gatgaagcta ttaagaacaa tagacttgct
    23101 ttacttaaac aagtatatga tactatgcta gaaatttgtg atttatctaa aattgtttat
    23161 aaataaaatt taaactattt ataagaaata aggctgtctc aaaatagaga tagtcttatt
    23221 tttcattgtt gagatgatga aaatatattt gatttcatag aaaaagagtg tctcataaac
    23281 taaaaagttc attttgagac acccatgttt tttgtaagta aatataataa actatatttt
    23341 atacttatca gttattttgt ttaaaatatt atcatttgaa tttacccatt cataaaaatt
    23401 gcctgatgat gcgtccgata ttttataaag catttcagga atatcactag ctttaaaatc
    23461 tccaagtgat tttcccaatc tagtcttacc tatttcaaaa ctgcctccaa caaataattc
    23521 aaatgtatct gtagatatac catcaacatt cttcttttta ccacataaac ctatgctacc
    23581 aacttgatga acaccacatg aatttaaaca tcctgatata tataacttag gtgctttatt
    23641 taaaatttct tcattatttt gtaatctaaa ataatctatg atttcatgta acattttctg
    23701 actattttgt atgcccattt gacaaattgg aacacctatg caagatatac tttgttctaa
    23761 ttgagaatta caactaatag ttttacttat ttctaatatt ctttttgctt catttccatt
    23821 taaatttaaa atatataatc cctctgtcat tcctagtcta atcataggat tttcaacatt
    23881 atctaattct ttaagtaaag tacttaaatc ctttgttaac aaaatacctc caactggatg
    23941 tatatataca gtgtataaac catcctgttt ttgaggtatt agtctagaat cttgtatatc
    24001 tattttaact ccaggtttag agtaatctat ttcttcaata ttgagttcaa gactaccatt
    24061 ttctttttct aatttatagt attctttaaa tttttctaag aatagttctt caccaagtga
    24121 ttcgaccata tatctaaccc tagctctgtt tttattttca taatttccat aatccataaa
    24181 gaatttaatc ataccttcaa cacaatataa ggcatctttc ggctttataa gttcatcaag
    24241 ttctaaagca actttgggtt gtttacctag acctccacct aagaaaactc tgaaatatgg
    24301 ttcatcattt ttcaaagtag caacaaatcc taagtcttgt atactacaat gagcagtatc
    24361 tgtgtaacaa ttagaataag aaacttttaa ttttctaggt aaatggtatg tagtagcatt
    24421 tttgataaaa tatttatcag tagcaactgc atatggagta acatcaaaga cttcactagg
    24481 gtcaactcca gataatggag ataatccgac atttctagga taattaccac caccacctct
    24541 agtgaatata tcatttttta ttccttcttc catgatatca acaattgaat ttatagataa
    24601 atcgtgcaat tgtatagctt gtctagttgt taagtgtatc ttatctaagt tgtgtttaat
    24661 agctatttca tagattttat taagttgata ctgagataaa actccagcag atactctaag
    24721 tcttatcata aaagattttt tgtctctttg agcataaaca ccatatccac cagaaaatcc
    24781 cttatattgc attttattta tttctccatt taaaaatttt gaagaatatt ctttaaaatt
    24841 aggtagttca tttaacaata tctttttaaa attatccatt atttcacctc attgttagtc
    24901 ttgtataaaa at
//
LOCUS       DAELLV010000030        24865 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.030, whole genome shotgun sequence.
ACCESSION   DAELLV010000030 DAELLV010000000
VERSION     DAELLV010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24865
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.030"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(251..1330)
                     /locus_tag="KPD58_001068"
     CDS             complement(251..1330)
                     /locus_tag="KPD58_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0264851.1"
                     /translation="MEGKVKKAFPGGNTSQGSYSFFDYIIPENVKRVFCLKGGPGVGK
                     SSLMKKIYKEFLHRGYDIDLYHCPSDPSSLDGLVIKKLGVVLMDATAPHTMDPKLPGA
                     LDEIINLGDYWNTEELEKNKQEISVYDKDISNSFKRAFKFLKSAEPIFRDIEEKYSDC
                     MDYGKVNLVTEEFIKRLFDGVKSTGNLKREKHLFGSAITPVGCLDYTENILKDVKKVY
                     YLDGEIGTGKTTLLNKVYKKAIEKGLNVEVYHYPLIPEKIETVLLTDLDIGITISTTF
                     KEEDTIDLNQFLNREKLLKYEEDIKFDEKVLDDLICWAVLNLKRAKSKHDIIESYYSP
                     NMRFDEVGKLRDKLIKRILKYEENL"
     regulatory      1618..1733
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1908..3278
                     /locus_tag="KPD58_001069"
     CDS             1908..3278
                     /locus_tag="KPD58_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0264852.1"
                     /translation="MKQKWIVLIIICIGVFMSTLDGSILNIANPTIAADFKINMSQIQ
                     WVVTAYMLVVTATMLFFGKLGDKVGSNRLYTLGFFIFTIGSFLCSMSNNLSTLISSRI
                     FQAVGASILMATGLGIVSNAFPANEKGKAIGITGAVVGIGNMSGPVIGGIILEHFGWP
                     SIFIINIPIGIIAVFLGIKFLPKPVLDEQNKSFDIPGLLLFASCTTLILLAMNEKGNT
                     RLYLGITALIIFLLLALREVKFEQSFIDLPLFKNRNFTVGNIIGVACYFPQMAVSFLL
                     PFYLEQLKNLSPMMAGYVMTVHPLIMVLIAPIAGSLSDKHGAKNILTASFSFMTISLV
                     GMALLKADSPLYLLIVCLVIFGLGLGAFSSPNNSSILADVPPQKQGYGGSFLATIRNL
                     SFALGTAFFSSFFAQSLTYNQKFKSHTSAYVIASNQSYWIAASVCFIGLILTVFFMRK
                     TDKSIS"
     gene            complement(3543..4154)
                     /locus_tag="KPD58_001070"
     CDS             complement(3543..4154)
                     /locus_tag="KPD58_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBG0264853.1"
                     /translation="MKIRSSEITMSAVNKSQYPAEGIPEIALAGRSNVGKSSIINTLL
                     NRRNFARTSQTPGKTRTINFYLINNEFYFVDLPGYGYAKIAKSEKEKWGGIMERYLES
                     RQELCSIFLLVDIRHEPTADDKLMYEWIKHFGYNCVVIATKADKISRGQYQKHISIIR
                     KKLQMESSEKVIPVSSLKKTGVEELWEEIVNQYNQHGYEITVD"
     gene            complement(4144..6507)
                     /gene="lon"
                     /locus_tag="KPD58_001071"
     CDS             complement(4144..6507)
                     /gene="lon"
                     /locus_tag="KPD58_001071"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HBG0264854.1"
                     /translation="MEQNYTKIDHELPLIPLRGLAIFPYMILNFDIGREISLKALDQA
                     MMDEELIFLTSQKEAEVDEPGEEDFYHVGTICKVKQMIKLPGDTVRVLVEGVSRGRVK
                     KIEQEDGYFRAVIEEIVFDSDNLDSETEVEIEAFVRNVFDAFEEYINIGNRVSPEILI
                     SLADIEDVDRFIDTIAANIYLKSSQKQEILEEFDIRKRLELIYSILLEEIDILKIEKK
                     ITLRVKKQMNKVQKEYYLREQLKAIQKELGEEEDINSEADEYREKLKKIKAPKTTKEK
                     IEKEIDKFSKISSMSPDVSVSRNYLDTIFSLPWNKETKDKLDITKAKDILDEDHYGLE
                     KVKERILEYLAIRTLAKSLKGPIICLVGPPGTGKTSIVKSIARALNRKFVRISLGGVR
                     DEAEIRGHRRTYVGSIPGRIINGVKEAQTKNPVFLFDEIDKMAADYKGDPASAMLEVL
                     DPEQNKDFVDHYLEIPFDLSKILFVTTANSLGNIPRPLLDRMEVIEVSGYIEEEKLNI
                     AKKYLLPKQIKEHALKENFIKIDDETLRSIINHYTREAGVRTLERTIGKICRKVAKKY
                     VEDPTLEEVVINKSDLETYLGKDMFKYQLAEVNPQIGLVNGLAWTEVGGVTLEVEVNV
                     LKGKGEIVLTGKLGDVMKESAKTGISYIRSIVDKFDIDPEFYKTNDIHIHIPEGAVPK
                     DGPSAGITMALAVISALTKRPVPGNIAMTGEITLRGRVLAVGGVKEKLLAAHRAGITK
                     VLIPKECEADLDEIPENVKEKMEFVLVEHMDEVLEQALLKSGENNEN"
     gene            6687..7199
                     /locus_tag="KPD58_001072"
     CDS             6687..7199
                     /locus_tag="KPD58_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBG0264855.1"
                     /translation="MLKLFLTFLKIGAFTFGGGYAMVPLIQDELVNKQKLLEQEEFID
                     YLSIAQSYPGVLAVNISVLLGYKLYGIPGVLICTLGSALPSFCIVLVLSYLYFKNSSS
                     KILDGFFKGVAPVVIALILYSFTSMFKKLPKSKTNILLLIIAVLSVGVFNISPIYLII
                     GGGVYSLCQK"
     gene            7187..7714
                     /locus_tag="KPD58_001073"
     CDS             7187..7714
                     /locus_tag="KPD58_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBG0264856.1"
                     /translation="MSEIILLFLIFLKIGAFSFGGGYAMLPFIQQEFISKYHFITNQE
                     FLDLLAISQSTPGPVAINSATFVGFKTAGFFGSLAATIGVTIFALIGLTIISKLFSKF
                     KNNKKVENLLTVLRPITLGFILSAAFSSAKEVNWDLYGIIAFIASFYLLYKGKIGSIA
                     IVFVFGILGILLTFL"
     gene            complement(7785..9035)
                     /gene="clpX"
                     /locus_tag="KPD58_001074"
     CDS             complement(7785..9035)
                     /gene="clpX"
                     /locus_tag="KPD58_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HBG0264857.1"
                     /translation="MSKYEEKRQLKCSFCGKNQDQVRRLIAGPNVYICDECVELCDEI
                     IQEEIEDTIDEDTTSLPKPKEMMEILNDYVIGQEKAKKALSVAVYNHYKRIYSKKSSS
                     KDIEIQKSNILLLGPTGSGKTLLAQTLARTLNVPFAMADATSLTEAGYVGEDVENILL
                     KLIQAADFDIEKAERGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPHQEFLKIDTTNVLFILGGAFDGLEKIIQKRGGDKTLGFGAKIESKKEL
                     DLGKLYEKVLPEDLLKYGIIPEFIGRIPVLATLELLDEDALMQILQEPKNALVKQYKK
                     LLELDDVELEFEEGALRAIAKKAIERNTGARGLRSIVESVMMETMFEVPSRDNIKKVI
                     VTEKSVNEDSVNPIIVLKDQEESA"
     gene            complement(9054..9638)
                     /gene="clpP"
                     /locus_tag="KPD58_001075"
     CDS             complement(9054..9638)
                     /gene="clpP"
                     /locus_tag="KPD58_001075"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBG0264858.1"
                     /translation="MALVPVVVEQTGRGERSYDIFSRLLKDRIIFLGDQVNDATAGLI
                     VAQLLFLEAEDPDKDIHLYINSPGGSITSGMAIYDTMQYIKPDVSTICIGMAASMGAF
                     LLAAGAKGKRLALPNSEIMIHQPLGGAQGQATDIEIHAKRILKIKETLNEILSERTGQ
                     PLEKIKMDTERDNFMSALEAKEYGLIDEVFTKRP"
     gene            complement(9788..11074)
                     /locus_tag="KPD58_001076"
     CDS             complement(9788..11074)
                     /locus_tag="KPD58_001076"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBG0264859.1"
                     /translation="MKAELIKKEGNKVTLKITVDNNKFEAAVNKAYNKSRNKYNIPGF
                     RKGKAPRIVIETQYGKGIFYNDAIEILFPEVYPEAIKELDIDPIDNPDLDIEEISKDN
                     GLVMVLNVEVKPEFELGNYKGIEIAKVENTVSDENVDAKLQEMVEKNARLVSVEDKAL
                     EDGDTAIIDFEGFENGVAFDGGKGENYNLVIGSNTFIPGFEEQLVGKKAGEEVEVNVT
                     FPEEYHSQDLAGKPVVFNVKINDVKVKELSALDDEFAKDTSEFDSLDELKADVRAKLE
                     EEAKNRADAETRNSVVEKVAENTEIEIPEVMIQHQIDNMLNELNYQLQYQGFGLQQLL
                     EMTGRTMEELREERKEDAKKLVKSSLVLEAITKAEGIEATEEEFKAELEKMASAYNME
                     VEKIEASLRDADKEDIKGQIKIRKTIDLLVDNATIA"
     gene            complement(11268..11738)
                     /locus_tag="KPD58_001077"
     CDS             complement(11268..11738)
                     /locus_tag="KPD58_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG0264860.1"
                     /translation="MKIGIVSDTHMMIKNMEKTIPYLKECDLIIHAGDNFTDSRYIHS
                     MTNVGIIAVKGNCDFDAVEEEVVFEVANKTIFLCHGDKYGVKYGTNMLEKKATEVDAD
                     IVIFGHTHTPFREIKDGVLYINPGSTSLPRGVSYKSFVIMDIEEDDIKIEEIRI"
     gene            complement(11738..13087)
                     /gene="rph"
                     /locus_tag="KPD58_001078"
     CDS             complement(11738..13087)
                     /gene="rph"
                     /locus_tag="KPD58_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBG0264861.1"
                     /translation="MTRSYDRSNEQIRPVKITRDYTKYAEGSVLIEMGETKVICTASV
                     EEKVPPFLRNSGTGWINAEYSMLPRATQQRKIRDASRGKIDGRTQEIQRLIGRAIRSV
                     VDLNKLGERTIWVDCDVIQADGGTRTASITGAFVAVAEAIYKLYKAKTIKEMPITNFV
                     SAISVGIVNGIHMLDLCYEEDSKAQVDMNIIMTDKCEFVEVQGTGEEKPFSRADLNLL
                     LELGEKGNKELIRLQREALGEISDEILGMDYGNEVVIATNNAHKLEEIGEILKDFEYK
                     VYSLKDVDLAGIEIVEDGKTFEHNALIKARTIAKKTKLIAISDDSGLEVDALGKKPGV
                     YSARYAGEHATDEENRKKLLKAMQNIPMSKRNARFVSAIAVVFPDGKEFVVRGICEGM
                     IGFEEKGKNGFGYDSLFIVKGYDKTFGEIPSVIKNSISHRANALKLMKQEFIKRVVK"
     gene            complement(13247..15271)
                     /gene="ligA"
                     /locus_tag="KPD58_001079"
     CDS             complement(13247..15271)
                     /gene="ligA"
                     /locus_tag="KPD58_001079"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBG0264862.1"
                     /translation="MSVKDEINKLIETINYHNERYYNQDSPEISDYEYDKLMKELISL
                     EEEHPELKRIDSPTNRVGGKPLDKFNQIVHKTPMLSLSNAFSEQDLRDFDKRVQEYVG
                     ENVEYVVEFKIDGLSVGLTYNNGEFEKGATRGDGVVGEDISQNLMTVKSIPLKINDKK
                     ELIVRGEVYISKDNFRKVNEQQEEQEQPLFANPRNLAAGSLRQLDPKLTAKRPLDIFV
                     FNMENIEEYDLESHSESLEYLEKLGFSVSQSYKVCKSIDEVIEHIEYWTDNRGNLDYE
                     IDGMVIKVNNIKQRDEMGYTAKSPRWAIAYKFPAEKKKTKIVDIIVEVGRTGTITPSA
                     ILEPVRLAGTTVSRATLHNEDYIREKDIKINDTVLVQKAGDIIPQVLEVIKEERTGDE
                     IDFKMPEKCPVCSEPTIRLEGEAAVKCINMSCPAQIRRGIIHFVSRDAMNIDGLGESI
                     ITLLLNEKIIQDVSDLYYIKKEDVVDLERMGEKSADNLINSIEKSKENDLWRLINGLG
                     IKFIGVKAAKILAYNFKDLDEVISATSEQLEELEEFGSIMANSVVEFFKEDKNMNVIN
                     KLKNVGVNTKSLDNTGEKVEKIFEGMKIVLTGTLPTLKRNDAKDMIEKRGGKATSSVS
                     KSTTFVLAGEEAGSKLTKANDLGIKVIDEERFLELLKLSSKDEVKAVLES"
     gene            complement(15457..16395)
                     /locus_tag="KPD58_001080"
     CDS             complement(15457..16395)
                     /locus_tag="KPD58_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBG0264863.1"
                     /translation="MKVLFTINYGKEKFDKIRELGYDVIHYSEDSIENNEDVNTADVL
                     VTYNPFERLDISKMKNLQYIQTTSVGIDQLPKEEVLKRNIKIANNRGGYSVPIGESIV
                     LYILEIYKNSAKFFKQQQNKEWKMDFSVSELYGQRVGFLGTGTISSEAAKRLKAFGVE
                     IWGVNTDGSSKKYFDKCFATEDMNIVFEECDIVVVAMPSTKSTDGIVNKDMFKLMKDG
                     SVFINVGRGNTVNQKDLEECVEKFRGVALDVFESEPLNKDNKLWECENVIVTPHNSWV
                     SDKNKERTFNMVYNNLKHYIMENKPVDNVVDISRGY"
     gene            complement(16497..16919)
                     /locus_tag="KPD58_001081"
     CDS             complement(16497..16919)
                     /locus_tag="KPD58_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HBG0264864.1"
                     /translation="MSFEKINNNNLDKIHNRKCIMLVNFNKKELSLIKNVSNFAGIRD
                     HIILSDKNGENIIRDILDGNISNECENGIKSKSVIFNNTPSTQVNAFLEGLKKVRINR
                     PFTAMVTETSIDWTLNNLIENLVQERNAMSLGKTIEHK"
     gene            17107..18390
                     /locus_tag="KPD58_001082"
     CDS             17107..18390
                     /locus_tag="KPD58_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugated bile salt MFS transporter"
                     /protein_id="HBG0264865.1"
                     /translation="MNTLLNNKFSIKNFSNAWVIVLGCMLIQAVPFSIVASIQPQFIS
                     YVVEGEGFSLASFSLIFTIGTIASAIASPFIGVLFSKVNIKILYLLGCIISSIAFATF
                     SICTELWQFYTVSCIVQIGATIISAIGIPVLISAWFDEESKGKALGFAFAGGSIGSIF
                     LQPLAVRLLATQGYSRSYLIFGIIALIVGIPVILFILRMPKDINEKVKSKGKKEKASD
                     TISPSVWGYTLKEAKKIKYFWIMTIGFIFVGIYVSSLSVQYPTYLRNYLNPILIGTVG
                     STFALFALIGNLFGGLLFDKFGQTKPLIISFVLVLIATLSLLLVDKSSTFAFVFAILK
                     GLSVFAYMIGPAYLTGSFFGQKEFGSIYGIVQLIFAVGFALGSTLFGLLVDNFGYRFS
                     WSIIILSVIIAYTLLIITSIGMKNLNKERLDKLMQ"
     gene            complement(18468..18983)
                     /locus_tag="KPD58_001083"
     CDS             complement(18468..18983)
                     /locus_tag="KPD58_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG0264866.1"
                     /translation="MNYFVIADPDKCIGCRTCMIGCVVAHSDEDIFYQNPDEINFNPK
                     LSVIKTKEVSAPIQCRHCEDAPCAKACPNGGIVRVGNTIKINEENCIGCKTCMLACPI
                     GAIDIVTLKDVDEGKLCFRERMTANKCDFCIESPEGPACVNVCPTKAFTIVKDEDIDS
                     KVKNKRKLAIL"
     gene            complement(18983..20359)
                     /locus_tag="KPD58_001084"
     CDS             complement(18983..20359)
                     /locus_tag="KPD58_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HBG0264867.1"
                     /translation="MIINIDKDLCTGCRECSKVCPVNAIEGEEGKPQEINLDRCVMCG
                     QCVQTCKSYASVIDEGFEFLQEKKQEREIPESINEPIFAAYNVCDIDKVKKALSDPNV
                     FTMVQCAPAVRVALGEDFGYYLGYLGAGKMAAALRALNFDRVYDTNFAADLTIMEEGS
                     ELIKRVTEGGTLPMFTSCCPAWVKFMEKNYPKLTNHLSSCKSPQQMGGAIFKTYGAEI
                     NNVDASKIFSVSIMPCTCKKYECDREEMDSSGYRDVDVVLTTRELAYLIKDMGIDFKN
                     LREEKFDSPLGSYSGAGTIFGVTGGVMEAAIRTGYELITGESIPDVEVKQVRGENGFR
                     KSTIKIGDLDLRVGVVSGLKNVIPVLEDLEKGKLDVDFIEVMTCPVGCVSGGGQPKIL
                     LEEYKELAYENRTKATYVHDEHLAIRKSHENPDIKKLYDEYLVEPLGEKSHHLLHTTY
                     CIGKEVAK"
     gene            complement(20384..20935)
                     /locus_tag="KPD58_001085"
     CDS             complement(20384..20935)
                     /locus_tag="KPD58_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG0264868.1"
                     /translation="MKNKIGSFVIADPNKCTGCRACEVACFTMHNQHNNVGYTVGTVD
                     IPVIPRLYLVKGDNFCMPIQCRHCEDAPCLNSCPQKAIVKENNIMSVNEEKCIGCKTC
                     LLACPFGAIDLLPQYEDGKEVEQVILDENKKIAYKCDLCKDNEKIACISACPQEALKL
                     VTPIDDKKAKNRQAALSLLLTNK"
     gene            complement(20993..21208)
                     /locus_tag="KPD58_001086"
     CDS             complement(20993..21208)
                     /locus_tag="KPD58_001086"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264869.1"
                     /translation="MQIHRELSKSENEYMCTKKEKQRYSKCEICGVLIDDTDKVYNNT
                     YNLCKKCKRNISTEKIVKYINIYDESK"
     gene            complement(21177..22010)
                     /gene="fdhD"
                     /locus_tag="KPD58_001087"
     CDS             complement(21177..22010)
                     /gene="fdhD"
                     /locus_tag="KPD58_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HBG0264870.1"
                     /translation="MNNIYSKTKLQENSKDKVFENSYSLVDYKYLNSFNVEVSKINEH
                     EVSIDNEEIIAEYPLSFILNDKYVNTFLCTPYNLEELIVGFLKTKGYIENKKSLLKFE
                     INESERFAKVTIQEKQKNQDEQIIFLNSLDYITCAPVKSNIKINYDTIYSIMDKNLNS
                     SELFKNTGGVHSVSVFNFDKKIVTCEDVARHNAMDKAIGFCVLNDITLKDKIVAVSGR
                     ISLEMILKAAMTQIPIVVSKSAPTNLSIELSKKLNITLIGFVRGRRMNIYANPQRVIK
                     K"
     gene            complement(22059..24203)
                     /gene="fdhF"
                     /locus_tag="KPD58_001088"
     CDS             complement(22059..24203)
                     /gene="fdhF"
                     /locus_tag="KPD58_001088"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(23787..23789),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HBG0264871.1"
                     /translation="MEKKILTVCPYCGAGCQLYLVVKDNKILRAEPANGRTNQESLCL
                     KGRYGWDFLNDPKILTPRLKKPMIRKQGVLEEVEWDEAINYTASRLNEIKEKYGPDSI
                     MGTGSARGPGNEANYIMQKFMRATIGTNNVDHCARVUHAPSVAGLAYSLGSGAMSNSI
                     PEIENADVLFIFGYNGADSHPIVANRIVKAKKNGAKLIVTDPRVTESARIADIHLPIK
                     GGTNMVLVNAFGNVLIEEGLYNKEFVQNHTQGFDEYKEIVKPYTAKYAEKITGIPEEL
                     IRKAMREYAKGKKAMILYGMGVCQFGQAVDVVKGLASIALLTGNFGRESVGIGPVRGQ
                     NNVQGACDMGALPNVYPGYQNVTDDKIREKFEKAWGVKLSPNNGYSLTQVPDLVLKEK
                     KLKAYYIFGEDPVQSDPDASEVREALDELEFVIVQDIFMNKTALHADVILPATSWGEH
                     EGVYTCADRGFQLMRKAIEPQGDVKPDWQIISEISTAMGYPMNYKNTKEIWDELRQLC
                     PSFLGATYEKIETQGCVQWPCKSESMEDKGTMYLYEGQKFSTPNGKGNLFAAEWRPPM
                     EVEDDEYPFSLCTVREVGHYSVRTMTGNCRTLSSLEDEPGRVQINSNDAEKLGIEDDE
                     LVRISSRRGSVITRATVTDRVKEGATYMTYQWWVGACNELTIANLDPISKTPEYKYCA
                     VKLEKLEDQELAEKCVREEYQSLKDKMTATNI"
     gene            complement(24454..>24865)
                     /locus_tag="KPD58_001089"
     CDS             complement(24454..>24865)
                     /locus_tag="KPD58_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBG0264872.1"
                     /translation="IDDYSKAINFFAKNKKVLISTIIVTFIQLSAYFSVSFWIYKAFN
                     LQGYSYIYILTLQAFLYMAISPIPTPGNVGANELAFFTIFKSVFPQPLMGYAVFLYGG
                     FMYYLILIGSGIFTVITHYRMKNRIGRNIVLSEN"
BASE COUNT         7352 a         4580 c         2816 g        10117 t
ORIGIN      
        1 ctaatttacc aatatcaagt atgcattttt agatgttctt aaaattatat aaactttcaa
       61 taaacattgt tttgatatta taaaaaaata taaattagga aaggaaataa taattatgaa
      121 aaacaaataa ttgctttgga gattattaat atcatttttt agcaaaaaaa agtaaaatgg
      181 catagcctca aagctacacc aatttactaa aaattatatc attattaaca ttaattttaa
      241 acatatacgt ttataagttt tcctcatatt ttaaaattct tttaattaat ttatctctta
      301 atttccctac ttcatcaaat ctcatattag gactatagta actttctata atatcatgtt
      361 tagacttggc tctcttaaga tttagtactg cccaacaaat taaatcatct aaaacttttt
      421 catcaaattt aatatcttct tcatatttaa gtagtttttc tctatttaaa aactgattta
      481 aatcaattgt atcttcttct ttaaaagtcg tacttatagt tatacctatg tctaaatcag
      541 ttaatagtac agtttcaatt ttctctggaa taagtggata atgataaact tctacattta
      601 aacctttttc aatagctttc ttatatactt tgtttaataa tgtagttttc ccagttccta
      661 tttctccatc taaataatag actttcttta catcttttaa aatgttttct gtataatcta
      721 aacaccctac aggagttata gctgaaccaa ataaatgctt ttctcttttt agatttcctg
      781 tgcttttaac tccatcaaat aaccttttta taaattcttc tgtgaccaaa tttactttgc
      841 cataatccat acaatcagaa tatttttctt ctatgtctct aaagattggt tctgctgatt
      901 ttaaaaactt aaatgctctt ttaaatgaat tactaatatc tttatcataa actgatattt
      961 cttgtttatt cttttctaat tcttctgtat tccagtaatc acctaaatta attatctcat
     1021 ccaaagctcc aggtagcttt gggtccatag tatgtggggc agttgcatcc ataagtacca
     1081 cgcctaattt ctttataact aaaccatcca acgaacttgg gtcagaagga caatgatata
     1141 aatcaatatc atatcctcta tgcaaaaact ctttataaat ttttttcata agtgaggatt
     1201 ttcctactcc aggtccaccc tttaaacaaa aaactctttt tacattttca ggaattatat
     1261 agtcaaaaaa agaatatgag ccttgtgatg tatttccacc cgggaatgcc ttctttacct
     1321 ttccttccat attagcgcct cctcataatt tacaatatct cttaattaag atattcatat
     1381 tataagaatt ttattacagc tttcttattt tgacaaattt aaaacttgcc atctaatact
     1441 aataacaggt taattttatc taattattat agattctcat actgtgcctt attctatcta
     1501 taaatacaat ttaagtgtcc atattgaaat atttgtattg taatacagct ggatattact
     1561 taaatccaat tgtttccatt ataattttat gttaaaataa tattacaaaa tacatctgtt
     1621 tttcttcata aacgggtgaa attccctatc ggcggtaaaa gcccgcgagc cttatggcat
     1681 aatttggtca tattccaaag ccaacagtaa aatctggatg gtagaagaaa atagtatatg
     1741 agtaccttta tgtaatttta catgagtaat ctatacaaat ccttcaacta ccgtatttat
     1801 tcatgaaatt agacacattc aaggtaccta atatacaggt gctttttttg ttgtttattt
     1861 tacaattata tcgtacttat aaaatctatt aagattggag tgttatcatg aaacaaaaat
     1921 ggatagtatt gattatcatc tgtattggtg tatttatgtc tactcttgat ggaagtatac
     1981 taaatatcgc aaatcctaca attgctgctg actttaaaat taacatgtca caaattcagt
     2041 gggttgtaac agcttatatg cttgttgtaa cagctactat gttatttttc ggaaaacttg
     2101 gcgataaagt aggttcaaat cgtctataca cattaggatt ttttatattt acaataggtt
     2161 cttttctgtg tagtatgtca aacaatttat caacattaat ttcttcaaga atctttcagg
     2221 cagttggagc aagtatttta atggctactg ggcttggtat agtttccaat gcatttccag
     2281 caaatgaaaa aggaaaagct ataggaatca ctggagctgt agttggtata ggaaatatga
     2341 gtggtcctgt aattggaggt ataattttag aacattttgg atggccttct atttttatta
     2401 tcaatatccc tattggtatt atagcagtct ttcttggcat taaatttctg cctaaacctg
     2461 ttttggatga acaaaacaag agctttgata ttccaggtct tctacttttc gcatcttgca
     2521 ctacgcttat attacttgca atgaatgaga agggtaatac aagattgtat ttaggtataa
     2581 cagcacttat aatcttctta ttgttggcat taagagaagt aaaatttgaa caaagcttta
     2641 ttgacctacc tctatttaaa aatagaaact ttactgtagg caatatcatt ggagtagctt
     2701 gttattttcc tcaaatggca gtatcttttc tacttccatt ttatttagaa caattaaaaa
     2761 atttatctcc tatgatggct ggttatgtaa tgactgtaca tccacttatt atggtactta
     2821 tagcacctat tgcaggttca ctttctgata aacacggtgc aaaaaacatt ctaacagcat
     2881 ctttttcatt tatgaccatc tcactagttg gaatggcact tttaaaagca gattctccat
     2941 tgtatctttt aattgtatgt ttagttattt ttggacttgg gctaggagca tttagttcac
     3001 caaacaatag tagtatttta gcagatgttc ctcctcaaaa acagggatac ggaggcagtt
     3061 ttcttgcaac tattcgtaat ttatcatttg ctcttggaac agcatttttt agtagttttt
     3121 ttgcacaaag tcttacatac aatcaaaagt ttaaaagtca cacatcagcc tatgttatag
     3181 ctagcaatca gtcttattgg attgcagcat cagtatgttt tatagggctt atactaactg
     3241 ttttctttat gagaaaaaca gataaatcta tttcttaaat gctatttatt gctgactaca
     3301 taagtcaata attttttagt tgaactaatt tatactaatt ttcccatacc ttactaaaat
     3361 ttattagtat ttagcaaaat tactgatatt tattgtttat aaacaataag gatgccaaaa
     3421 aaagttcatt aaaactattt taaggcatcc tatattttgc tttaatatta atataattaa
     3481 aactaactat tattacattt attattcaaa taaatcttat gtcaatattt aacattttaa
     3541 atttaatcta ctgttatttc atatccatgt tgattgtatt gatttacaat ctcttcccat
     3601 aattcttcaa caccagtctt ttttagtgaa gaaacaggaa ttactttttc actactttcc
     3661 atttgcaatt tttttcttat tatacttatg tgtttttgat attgtcctct agatatttta
     3721 tctgccttag ttgcaataac tacacagtta tatccaaagt gctttatcca ttcatacata
     3781 agcttatcat ctgctgtagg ctcatgtcta atatctacaa gtaggaatat agaacataac
     3841 tcttgtctac tttctagata tcgttccatt atacctcccc acttttcttt ttcagactta
     3901 gctatctttg catatccata gcctggaagg tctacaaaat aaaattcatt atttattaga
     3961 taaaaattta tagttctagt ttttcctggt gtttgactgg ttctagcaaa gtttcttctg
     4021 ttaagcaatg tatttattat tgatgatttt ccaacatttg acctaccagc caatgctatc
     4081 tctgggattc cttctgctgg atactgtgat ttattcacag cactcatagt tatctctgaa
     4141 cttctaattt tcattatttt caccactctt taataaagct tgttctaata cttcatccat
     4201 atgttctaca agtacaaact ccattttttc ttttacattt tctggtattt catctaaatc
     4261 agcttcacat tctttaggaa ttaatacttt tgttatacca gctctatgag ctgctaataa
     4321 cttttcttta actcctccta ctgctaagac tctacctctt aaagttatct ctcctgtcat
     4381 tgctatattt ccaggcacag gtcttttagt aagtgctgag attactgcaa gagccatagt
     4441 tataccagct gatggtccat cttttggaac agcaccttct ggaatgtgta tatgaatatc
     4501 attcgtctta taaaattcag ggtctatatc aaacttgtcc actattgacc ttatataaga
     4561 gatacctgtc ttcgcagatt ccttcataac atctcctaat tttccagtta atacaatttc
     4621 gcctttacct ttaagtacgt ttacttctac ttcaagtgta actccaccaa cttctgtcca
     4681 cgcaagacca tttactaatc ctatttgagg gttaacttct gcaagttgat acttaaacat
     4741 gtcttttcca agatatgtct ctaggtcaga cttatttata acaacctctt caagagttgg
     4801 gtcttcaacg tattttttgg caacctttct acaaatctta ccaatagttc tttctagtgt
     4861 tcttacacct gcttctcttg tataatggtt gattatactt cttaaagttt catcatctat
     4921 ttttataaag ttttctttta aagcatgttc tttaatctgt tttggaagta ggtatttttt
     4981 tgctatattt aatttttctt cttctatata tccagatact tctattactt ccattctatc
     5041 taataatggt cttggtatat tacctagact atttgctgtt gtaacaaata gtatttttga
     5101 taaatcaaaa ggtatttcta agtaatggtc cacaaaatcc ttattttgct ctgggtctaa
     5161 tacttctagc attgctgaag ctgggtcacc tttataatct gctgccatct tatctatctc
     5221 atcaaataag aatacaggat tttttgtttg ggcttctttt accccattaa ttattcttcc
     5281 tggaattgaa cctacataag ttcttctatg acctcttatt tctgcttcat ctctaactcc
     5341 acccaaagat attcttacaa attttctatt taatgctctg gcgattgatt ttactataga
     5401 tgtctttcct gttcctggag gaccaactag acatattata ggccctttaa gtgacttagc
     5461 aagagttctt atagctagat actctagtat tctctcctta actttttcta atccataatg
     5521 gtcttcatct aatatatctt ttgccttagt tatatctaat ttatcttttg tctctttatt
     5581 ccatggcaat gagaatatag tatctaaata gtttctactt acagatacat ctggagacat
     5641 agaagatatt tttgaaaatt tatctatctc tttttctatt ttctctttag ttgtcttagg
     5701 tgcttttatt tttttaagct tttctctata ctcatctgct tcagagttta tatcttcttc
     5761 ttcacctaat tctttctgta tagcttttaa ttgttctcta agataatatt ctttttgaac
     5821 cttattcatc tgttttttta ctcttaaagt tattttcttt tctattttta atatatctat
     5881 ttcctctaat aatatagaat aaataagttc aagtcttttt ctaatatcaa attcttctaa
     5941 tatttcttgt ttttggcttg attttagata tatgtttgca gctatcgtat ctataaatct
     6001 atctacatct tctatatcag ctaaagatat taatatttct ggtgataccc tatttccaat
     6061 atttatatac tcttcaaagg catcaaatac atttcttaca aatgcttcaa tttcaacttc
     6121 tgtttcacta tccaaattat cactatcaaa tactatttct tctattactg ctctgaaata
     6181 cccatcttct tgttctattt tctttactct accccttgat acaccttcaa ccaaaacacg
     6241 aactgtatct cctggtaatt ttatcatttg cttaacttta cagattgttc ccacatgata
     6301 aaaatcctct tctcctggct catctacctc tgcttctttc tgagaagtta aaaatataag
     6361 ttcttcatcc atcatagctt ggtctaaagc tttaagagat atctctctac ctatatcaaa
     6421 atttaatatc atatatggaa atatagctaa tcctcttaga ggaattaatg gtaattcatg
     6481 atctatttta gtataatttt gttccatcaa cattactcct tttctagtct aattgctttg
     6541 cttttaatat tatatatttg aacaagttaa ttttcaacat taatgataaa ttcttagaat
     6601 actatcatac tatattatct ttttattatg atataatatt aatattagta tacttagatg
     6661 ttatttatct tttggaggtt tctattatgt tgaaattgtt tttaaccttt ttaaaaattg
     6721 gtgcgtttac atttggtggt ggatacgcta tggttcctct tatacaagat gaacttgtaa
     6781 acaaacaaaa acttttagag caggaagaat ttattgatta tttatccata gctcaaagtt
     6841 atcctggagt actggcagtt aatatatctg tattattagg atataaactt tatggtatac
     6901 caggagttct tatatgtacc ttaggctctg ctttaccatc attttgtata gtcttagtct
     6961 tatcgtatct atactttaaa aattcatcat ctaaaatttt agatggattt tttaaaggag
     7021 ttgcgcctgt tgtaatagct cttatattgt attcttttac gagcatgttc aagaaactac
     7081 caaagtctaa aactaacata ctactattaa ttatagcagt tttatctgtt ggtgtattca
     7141 atataagccc tatttattta ataattggag gaggtgtata tagtctatgt cagaaataat
     7201 actactcttc ttaatattcc ttaaaatagg ggcatttagt tttggtggcg gttatgccat
     7261 gctaccattt atccagcaag aatttatatc taaatatcac tttataacta atcaagagtt
     7321 tttggattta cttgctatat cacaatctac accaggtcct gtagcaataa atagtgctac
     7381 atttgtagga tttaagactg ctggcttctt tggttctcta gctgcaacta ttggtgttac
     7441 aatttttgca ttaataggtc taactattat atcaaagtta ttttctaaat ttaaaaataa
     7501 taaaaaggta gaaaacttat tgacagttct tagaccaatt actctaggat tcatattatc
     7561 agcagctttt tcatctgcca aagaagttaa ttgggattta tatggaataa ttgcatttat
     7621 agcatcattt tatcttttat ataaaggaaa aattggttcc attgcaattg tattcgtctt
     7681 tggtattcta ggtatattac tcacattttt ataataatga tacctatttt atatttagca
     7741 tattaacata aaaaaaggag cttttaaagc tccttttcat catcttatgc actttcctct
     7801 tgatccttaa gtactatgat aggatttact gagtcttcgt tcacagattt ctctgttact
     7861 ataaccttct ttatattatc tctagatggt acttcaaaca tagtttccat cattacgctt
     7921 tcaactatac ttctaagtcc tctagctcct gtatttcttt ctatggcctt tttagcgatt
     7981 gcacgaagag caccttcttc aaattctagc tcaacatcat ctaactccaa taacttttta
     8041 tactgtttta ctagagcatt tttaggttcc tgtaatatct gcattaatgc atcttcatcc
     8101 aacaactcta aagtagctaa aactggtatt cttcctataa actctggtat tataccatat
     8161 tttaataaat cctctggaag tactttttca tatagcttcc ctaaatctaa ttctttttta
     8221 ctttctattt tagctccaaa acctagtgtt ttatctccac cacgtttttg aattatcttc
     8281 tctaatccat caaaagctcc acctaatata aataatacat tagtagtatc tatctttaaa
     8341 aattcttgat gaggatgttt tcttcctcct tgtggtggaa catttgcaac agtaccttct
     8401 aatattttta gaagtgcttg ttgaacacct tcaccactaa catctctagt tatagatggg
     8461 ttttcagatt ttcttgcaat cttatctatc tcatctatat atattatacc tctttcagct
     8521 ttttctatat caaaatcagc agcttgaata agttttaaaa gtatattttc aacgtcttct
     8581 ccaacatatc cagcttcagt caatgatgta gcatctgcca ttgcaaaagg tacatttaga
     8641 gttcttgcta gagtttgagc aagtaatgtc tttccagaac ctgtaggtcc taataaaagt
     8701 atattactct tttgaatttc tatatcctta ctactagatt ttttactgta tattctctta
     8761 taatgattat aaaccgcaac tgataaagct tttttagctt tttcttgacc tattacataa
     8821 tcatttagaa tctccatcat ttcctttggt tttggtaaag atgtagtatc ctcatctata
     8881 gtatcttcta tctcttcttg aataatttcg tcacaaagtt caacacactc gtcacaaata
     8941 tatacatttg gacctgcaat taatcttctt acttgatctt gatttttacc gcagaatgaa
     9001 cactttaatt gtcgtttttc ttcatattta gacatattaa cacctctctc tatctacggt
     9061 ctctttgtaa atacttcatc tattaaacca tattctttgg cttctagtgc actcatgaag
     9121 ttatcacgtt cagtatccat tttaattttt tctaatggct gaccagttct ttcagataat
     9181 atctcattta aagtttcttt tatttttaaa attctctttg catgaatttc tatatctgtt
     9241 gcttgacctt gagctccacc taatggttga tgtatcatta tttcactatt tggtagtgct
     9301 agtctttttc cttttgctcc tgctgccaac aagaatgctc ccatagaagc agccatacct
     9361 atacatatag tacttacatc tggtttgatg tattgcatag tatcatatat agccattcca
     9421 gaagttatac ttcctcctgg agaatttata tataaatgta tatctttgtc tgggtcttca
     9481 gcttctaaaa ataatagctg tgctactata agccctgctg tggcatcgtt aacttgatct
     9541 cctaagaaga ttattctgtc ttttaatagt ctagaaaaaa tatcataaga tctttctcct
     9601 cttcctgttt gttcaacaac tacaggtact aatgccatgg tttcataccc cctttattct
     9661 atataattta cagtagcttt ttttaagcta ctgtaaatta ttatacgcat ttaactaaat
     9721 tatatttgca catcattata tactaaacat ttcataacgt caactatatt agttagcgtt
     9781 tattttatta agctattgtt gcattatcaa ctaataaatc tattgttttt cttattttta
     9841 tttgaccttt tatatcttct ttgtcagcat ctcttaaaga agcttctatt ttttctactt
     9901 ccatattgta agcagaagcc attttttcta actcagcctt aaattcttct tctgttgctt
     9961 caattccttc agctttagtt attgcttcta atactaaaga agacttaact aattttttag
    10021 catcttcttt tctctcttct cttaattctt ccatagttct accagtcatc tctagtaatt
    10081 gttgtaatcc aaatccttga tattgtaatt ggtagttaag ttcatttaac atattatcta
    10141 tttgatgttg tatcataact tctggtattt ctatttctgt attttcagca actttttcta
    10201 ctactgaatt tctagtttct gcatctgctc tatttttagc ttcttcttct aacttagctc
    10261 ttacatctgc ttttaattca tctaaagaat caaattcact agtatcttta gcaaattcat
    10321 catcaagtgc agataattct tttactttta catcatttat ttttacatta aatacaactg
    10381 gttttccagc taaatcttgt gagtgatatt cttctgggaa agtaacatta acttctactt
    10441 cttcaccagc ttttttacct actaattgtt cttcaaatcc tggtataaat gtgtttgaac
    10501 ctataactaa gttataattt tcacctttac caccatcaaa agctacacca ttttcaaaac
    10561 cttcgaagtc tattatagct gtatctccat cttctaaagc tttgtcttct acacttacta
    10621 atctggcatt tttttcaacc atttcttgta gtttagcatc aacattttcg tcacttacag
    10681 tattctcaac cttagctatt tctataccct tgtagtttcc taattcaaac tctggtttaa
    10741 cttctacatt aagaaccata accaacccat tgtctttact tatttcttct atatctaaat
    10801 ctggattgtc tattgggtct atgtctaatt ccttgatagc ttctggataa acttctggga
    10861 ataatatttc tatagcatca ttgtagaata taccttttcc gtattgagtc tctataacaa
    10921 ttcttggagc tttacctttt ctaaatccag gtatattgta tttatttctg cttttattat
    10981 aagccttatt tactgctgct tcaaatttat tgttatctac tgttattttt aaagtaactt
    11041 tattaccctc ttttttaatt agttctgctt tcatccttct aatcctccta gtgatattta
    11101 tgtaataccc gataacggta ttataattca ttctatttat aattcaacca cttaattata
    11161 tcacataagt ttttttattt acaagtgttc cattaatatc gttgtactta tttgataaaa
    11221 tttaattttt tgagcgtttt ttgccatttt tttagataaa tacaccttta tatgcgtatt
    11281 tcttcaattt ttatgtcatc ttcttctata tccataatga caaaactttt atacgatact
    11341 cctcttggaa gtgaagtgct tccaggattt atgtaaagta caccatcttt aatttctcta
    11401 aatggggtat gggtatggcc aaatataact atatcagcat caacttcagt tgcctttttt
    11461 tctaacatat tagttccata ttttacacca tacttatctc catggcataa gaatatggtt
    11521 ttatttgcca cttcaaagac aacttcttcc tcaacagcat caaagtcaca attaccttta
    11581 actgcaataa tacctacatt agtcatgcta tgaatatatc ttgaatcagt aaagttatct
    11641 cctgcatgaa ttattaggtc acattctttc aaatatggta ttgttttttc catattttta
    11701 atcatcatat gtgtgtcgct tactatgcct attttcatta cttcactacc ctttttataa
    11761 actcttgttt cataagtttt agtgcgtttg ctctatgact gattgaattt tttataacac
    11821 taggtatttc accaaaagtc ttatcatatc cttttactat aaataagcta tcatatccaa
    11881 aaccattttt acctttttct tcaaatccta tcattccttc acaaattcct cttactacaa
    11941 attcttttcc atcaggaaaa actactgcta ttgcagagac gaatcttgca tttcttttgc
    12001 tcattggtat attttgcata gcttttaaca gtttttttct attttcttca tctgttgcat
    12061 gttctcctgc atatctagct gaataaacac caggtttttt gcctaaagca tctacttcta
    12121 agccagaatc atctgatatt gctataagtt tagtcttttt tgctattgtt ctagctttta
    12181 tcaacgcatt atgctcaaat gtttttccat cttctactat ctctatacct gctaaatcaa
    12241 catcttttaa agagtacact ttatattcaa aatcttttaa gatttcacct atttcttcta
    12301 gcttatgagc attattagta gcaatcacta cttcattacc ataatccata cccaatatct
    12361 catctgatat ttcacctaaa gcttctcttt gaagccttat taattcttta ttcccttttt
    12421 cacctaactc taacaataaa tttaaatctg ctctcgaaaa aggtttctct tctcctgtcc
    12481 cctgtacttc tacaaactca cacttatcag tcattattat attcatatct acctgtgctt
    12541 tagaatcttc ttcataacat aaatctagca tgtgtattcc atttacaata ccaacactaa
    12601 ttgcagacac aaaatttgtt attggcatct cttttattgt ctttgcttta tataatttat
    12661 atattgcttc tgcaactgct acaaatgccc ctgttataga tgcagttcta gttccaccat
    12721 ctgcctgaat cacatcacaa tctacccaaa tagttctttc tcctaattta ttcaagtcta
    12781 caacagacct tattgctctt cctattaacc tctgtatttc ttgtgttctt ccatcaattt
    12841 ttcccctaga tgcatctctt atctttcttt gttgtgttgc tcttggtagc atagaatatt
    12901 cagcatttat ccatccagta ccactatttc ttaaaaaagg aggaactttt tcttccactg
    12961 atgcagtgca tataaccttt gtctctccca tttctataag aacagaacct tctgcatact
    13021 ttgtataatc tctagttatc tttacaggtc ttatctgttc attacttcta tcataacttc
    13081 tggtcatttt ttatatcctt tctcatattt tttatttttt ataaatttat ttttataatt
    13141 tttaattttg tcaaatatta attattgtca tttttcgtaa tcttattagc ttactgaact
    13201 attctatcat taattatagt agttttctac taaattaagc gactttttaa ctctctaata
    13261 cagcttttac ttcatctttc gatgataact ttaataattc taaaaatctc tcttcatcta
    13321 taactttaat tcctaaatca tttgctttag taagtttaga accagcttct tcacctgcca
    13381 aaacaaatgt tgtcgattta ctaacactag atgtagcttt tccacctctt ttttctatca
    13441 tatcttttgc atcatttctt tttaatgtag gaagtgtacc agtcaatacg attttcatcc
    13501 cttcaaatat tttttctact ttttcacctg tattatccaa tgattttgta tttactccta
    13561 cattttttaa cttgtttata acgttcatat ttttgtcttc cttaaaaaac tcgacaacgc
    13621 tgtttgccat tatacttcca aattcctcta attcttctag ttgctcacta gttgcagaaa
    13681 ttacttcatc taaatcctta aagttatatg caagtatctt agctgcttta actcctataa
    13741 atttaatacc taatccattt ataagtctcc ataaatcatt ttcttttgat ttttcaattg
    13801 aatttattaa attgtcagca gatttttcgc ccattctctc taggtcgact acatcttcct
    13861 tttttatata atataaatca gatacatctt gaataatttt ttcatttaat agcagtgtaa
    13921 ttatagattc tcccaatcca tctatattca ttgcatccct tgacacaaag tgtataattc
    13981 cccttcttat ttgggctggg caagacatat tgatacattt tacagcagcc tctccttcta
    14041 atcttattgt tggctctgaa caaactggac acttttctgg cattttaaaa tctatttcat
    14101 ctccagtacg ttcttctttt ataacttcta aaacttgagg tattatgtcc cctgcttttt
    14161 gaactaatac tgtatcattt atttttatat ctttttctct tatataatct tcattgtgaa
    14221 gtgtagctct acttacagta gtacctgcaa gtctgactgg ttcaagaatt gctgaaggtg
    14281 ttattgtacc agttctccca acttctacta ttatatctac tattttagtt ttcttctttt
    14341 ctgctgggaa tttatatgca attgcccatc taggactttt ggctgtatat cccatttcgt
    14401 ctctttgttt tatattgttt acttttatta ccataccatc tatttcgtag tctaaattac
    14461 ccctgttatc tgtccagtat tcaatatgct caataacttc atctatactt ttacaaactt
    14521 tgtaactctg acttactgaa aatcctagtt tttctaagta ctctagtgat tcactgtgac
    14581 tttctaaatc gtattcttct atattttcca tattaaatac aaatatatct aatggtctct
    14641 ttgctgttag ttttgggtct aattgtctca atgaaccagc tgcaagattc cttggattag
    14701 caaataatgg ctgttcttgt tcttcttgtt gttcatttac ttttctaaaa ttatcttttg
    14761 atatatatac ttctcctcta actattagtt cttttttatc atttatctta agaggtatgc
    14821 tttttactgt cattaagttt tgagaaatat cttctcctac aactccatct cctctagttg
    14881 ctcctttttc aaactctcca ttgttataag ttagacctac agaaagacca tctattttaa
    14941 attcaacaac atattcaaca ttttctccca catattcttg aactctctta tcaaaatctc
    15001 tcaaatcttg ctctgaaaaa gcatttgata aactcaacat aggagtttta tgtacaattt
    15061 gattgaactt atctaatggc ttacctccta ccctatttgt tggagaatca attctcttta
    15121 gttctggatg ttcttcttct aaagaaatta actccttcat taacttatcg tattcataat
    15181 ctgatatttc tgggctatct tgattataat acctttcatt atggtagttt attgtctcta
    15241 taagtttatt aatttcatct tttacgctca ctttttgcac ctcttcatat ttttaaaatt
    15301 aaattatttt gaaatttccc tataattaat tatctcctat ttatatattt tttagtatat
    15361 cacttttttg gctttaaatt tattacaaaa aaaactctta ttagtatctt taatacgact
    15421 aataagagtt tttattttta tcaaaatttt ttatttttaa tagcctcttg atatgtctac
    15481 aacattatct acaggtttat tctccattat atagtgtttc aaattattat aaaccatatt
    15541 aaatgttctc tctttgtttt tatctgacac ccaagaatta tgaggagtta ctatgacatt
    15601 ttcgcactcc cacaacttat tgtccttgtt taaaggttca ctttcaaata cgtctaatgc
    15661 tacccctcta aatttctcta cacattcttc taaatctttc tgattaactg tatttcctct
    15721 acctacattt ataaatacag aaccatcttt cataagttta aacatatctt tgtttactat
    15781 tccatctgta ctttttgtag aaggcattgc tacaactact atatcacatt cttcaaatac
    15841 aatattcata tcctcagtag caaaacactt atcaaagtat tttttactac ttccatcagt
    15901 attgacaccc catatttcta caccaaaagc ttttaatctt tttgctgctt ctgaagatat
    15961 agtacctgtt cctaaaaatc caactctttg tccatacaat tctgatacag aaaaatccat
    16021 cttccattct ttattttgtt gttgtttaaa aaattttgca ctatttttat atatttctaa
    16081 aatatacaat acaatacttt caccaatagg tacactatat ccacctctat tattagctat
    16141 cttaatattt ctcttcaata cctcttcttt tggaagttgg tctataccta cactagttgt
    16201 ctgtatatat tgtaaatttt tcatcttaga aatatctaat ctctcaaatg gattgtatgt
    16261 aactagtaca tctgctgtat ttacatcctc attattttca atactatctt cagagtaatg
    16321 tatgacatca tagcctaatt ctcttatttt atcaaatttt tcttttccat aatttattgt
    16381 aaataaaact ttcatctcaa atctcctcca tctacccaca tttaaaattt tatattctaa
    16441 aatataatat gatactcaat gtacattagt taaaattact ataagatatc ttaaatttac
    16501 ttatgttcaa ttgtttttcc taaagacata gcattacgtt cttgaactaa attttctatt
    16561 aaattattca atgtccaatc aatagatgtc tctgttacca tagctgtaaa aggtctattt
    16621 attctcactt tttttagccc ttctaaaaat gcatttactt gtgtgcttgg agtgttatta
    16681 aatattacag acttactctt aatcccattt tcacattcat ttgatatatt tccatctaaa
    16741 atgtctctta ttatattctc tccattttta tcacttaaga tgatatggtc tcttattcca
    16801 gcaaagttac ttacgttttt aattaatgat aattcttttt tattaaagtt tacaagcata
    16861 atacacttcc tgttgtgtat tttatctagg ttattattat ttattttttc aaatgacatt
    16921 ttatccatcc tttcatttat ttatatactt taatttaccc catacattta aaataatcaa
    16981 tttttatagt taatttttat attaataccc aaattatatt taaatagtta ggctatccaa
    17041 aactaatata taaaattaga ataattagat taatatctac aaatcataac ctaaaggaga
    17101 gataccatga atacattact aaacaataaa tttagcatta aaaacttttc aaatgcttgg
    17161 gttatagtac ttggttgtat gttgatacaa gctgttccat ttagtattgt agcaagtata
    17221 cagcctcaat ttataagtta tgttgtagaa ggagaaggct ttagtttagc ttctttttct
    17281 ctaatattca ctattggtac tattgcctca gcaattgcat caccttttat aggtgtatta
    17341 ttttctaaag tcaatattaa gatactatat ttattaggtt gtataatttc tagtattgct
    17401 tttgctactt tttctatatg tactgaacta tggcaatttt acactgtatc atgtattgtt
    17461 caaattggcg ctacaattat atctgctata ggaatacctg ttttaataag tgcttggttt
    17521 gatgaagaaa gcaaaggaaa agctttgggc tttgcttttg ctggtggttc tattggtagt
    17581 atatttctac aaccacttgc agttcgctta ttagcaactc aaggatacag tcgttcttat
    17641 ctaatatttg gaattatagc attaatagtt ggtatacctg taatattatt tatactcagg
    17701 atgcctaaag atattaatga aaaggtaaaa tctaaaggta aaaaagagaa agcatctgat
    17761 acaatttctc cttcagtatg gggttataca ttaaaagaag ctaaaaaaat taaatatttt
    17821 tggattatga ctattggatt tatatttgtt ggtatatatg tttcatcatt atctgtacaa
    17881 tatccaactt atcttagaaa ttatctaaat cctattctaa taggaacagt aggctccaca
    17941 tttgctttat ttgctctcat tggtaattta tttggaggat tattgtttga taaattcggt
    18001 caaacaaagc cacttattat atcttttgta ttggttctta tagcaacttt atcattacta
    18061 ttggtagata aatcttctac ttttgcattt gtatttgcca ttttgaaagg tctttctgta
    18121 tttgcttata tgattggtcc tgcttattta actggctctt tctttggtca aaaggagttt
    18181 ggtagcatat atggaatcgt acaattaatt tttgcagtgg gatttgcatt aggttcaaca
    18241 ttatttggat tgttagttga taattttgga tacagatttt cttggtctat tataatttta
    18301 tcagttataa ttgcctatac tttacttatt ataacttcta taggaatgaa aaatttgaat
    18361 aaagaaagat tagataaact aatgcaataa ttgattttta gatatataaa tattataata
    18421 aaaaagatgg agcttttaat cataaataaa actccatctt ttttatatta caatatagct
    18481 aattttctct tatttttaac tttactatct atatcctcat ccttaactat agtaaatgcc
    18541 ttagttggac aaacatttac acatgcaggc ccttctggcg attctataca aaaatcacac
    18601 ttatttgctg tcattctttc tctaaagcaa agctttcctt catctacatc ttttaatgta
    18661 actatgtcta ttgcacctat aggacatgct aacatacaag tcttacatcc tatacaattt
    18721 tcttcattga tttttattgt gttaccaacc ctaactatac caccatttgg acatgccttt
    18781 gcacaaggag catcttcaca atgtctacac tgtattggag cacttacttc ttttgtcttt
    18841 atgacagaaa gttttggatt aaaatttatt tcatctggat tttggtaaaa aatatcttca
    18901 tctgaatgtg caactacaca tcctatcata caagttctac atcctatgca tttatcaggg
    18961 tctgctataa caaaatagtt catcacttag ccacttcctt tcctatacaa tatgtagtgt
    19021 gtaatagatg atgagatttt tctcctaatg gttctaccag atattcatca tacagttttt
    19081 taatatcagg attctcatga gattttctaa ttgctaaatg ttcatcatgc acgtatgttg
    19141 cttttgttct attttcataa gccaattcct tatattcttc tagtaaaatt tttggttgac
    19201 ctccaccact tacacaccca acaggacaag tcataacttc aataaaatct acatctaatt
    19261 ttcctttttc caagtcttca agcactggta ttacattttt taatccactt actactccaa
    19321 ctcttaagtc taagtctcct attttaatag ttgactttct aaaaccattt tctcctctta
    19381 cttgttttac ttctacatca ggaatacttt ctccagttat aagctcatat cctgttctta
    19441 tagcagcttc cataacccca ccagtaacac caaagatagt tccagctcca ctataactac
    19501 ctaaaggact atcaaatttt tcttctctta aatttttaaa atcaattccc atgtctttta
    19561 ttaaataagc tagttctcta gtagttaata ctacatctac atctctatat ccactagaat
    19621 ccatttcttc tctatcacat tcatattttt tacatgtaca tggcattata gaaacactaa
    19681 atatctttga tgcatctaca ttatttatct ctgcaccata agttttaaat attgcaccac
    19741 ccatctgctg aggtgattta caagaagata aatgattagt taatttagga taattttttt
    19801 ccatgaattt tacccaagct ggacaacaag atgtaaacat tggaagagtt ccaccttctg
    19861 tcactctttt aattaattca ctaccttctt ccattatagt taaatctgct gcaaaattag
    19921 tatcgtagac tctatcaaaa tttaaagctc taagtgctgc tgccattttc ccagcaccta
    19981 agtatcctaa ataatatcca aaatcttctc ctaaagcaac tctaactgct ggtgcacatt
    20041 ggaccatagt gaatacattt gggtcactta aagctttctt caccttatct atatcacata
    20101 cattgtatgc tgcaaatata ggttcattta tagactctgg tatctctctt tcttgttttt
    20161 tctcttgtaa aaactcaaat ccttcatcta taactgaggc ataagattta caagtttgaa
    20221 cacattgacc gcacataacg catctatcta aatttatctc ttgtggcttt ccctcttcac
    20281 cttcaatagc atttactgga cagacctttg aacattctct acatccagta cataaatcct
    20341 tatctatatt tattatcata aacgtcacct acccctgtat cctttattta tttgttaaca
    20401 ataaactaag tgcagcttgt ctattcttag cttttttgtc atctattgga gtaacaagtt
    20461 ttaatgcttc ctgaggacat gcacttatac aagctatttt ttcattgtct ttacataagt
    20521 cacatttata agctatcttc ttattttcat ctaaaattac ttgttctact tctttaccat
    20581 cttcatattg tggaagtaag tctatagctc caaaaggaca tgctagcaga caagttttac
    20641 atcctataca cttttcttca tttacagaca taatattgtt ttctttaact atagcttttt
    20701 gtggacatga gtttaaacaa ggagcatctt cacaatgtct acactgtatt ggcatacaga
    20761 aattgtcacc ttttactaag taaagtctag gtataacagg tatatcaact gttccaactg
    20821 tataacccac attattatgc tgattatgca ttgtaaaaca agctacttca caagctctac
    20881 aacctgtaca tttgtttggg tctgctatta caaaagagcc tattttattt ttcatattat
    20941 cccacctttc aatttgtata tttccaacca tctagttatt ttcatcatat gtctacttac
    21001 tttcatcata tatatttata tatttcacta ttttctcagt acttatattt cgtttacatt
    21061 ttttgcacaa attgtatgtg ttattataca ccttatcagt atcatcaatt aatactccac
    21121 agatttcaca ctttgaatat ctttgctttt cttttttagt acacatatat tcattctcac
    21181 tttttgataa ctctctgtgg atttgcataa atattcatcc gtcttcctct tacaaatcca
    21241 ataagcgtta tatttaattt tttagaaagc tctattgata aattagtcgg tgctgatttt
    21301 gatactacaa taggaatttg agtcatagct gcttttaata tcatttccaa tgatattctt
    21361 ccacttacag ccacaatttt atcttttaat gttatgtcat ttaaaacaca aaatccaata
    21421 gccttatcca ttgcattatg ccttgctaca tcttcacaag tgactatttt tttatcaaaa
    21481 ttaaatacag atacgctatg taccccacca gtatttttaa ataattctga tgaatttaaa
    21541 tttttatcca ttatactgta aatcgtatca taatttattt ttatattgct ctttacagga
    21601 gcacaggtta tatagtctag tgaatttaaa aatataattt gctcatcttg atttttttgt
    21661 ttctcttgaa tagttacttt tgcaaaacgt tcactctcat ttatctcaaa ttttaataag
    21721 ctttttttgt tttctatgta tccttttgtt ttcaagaatc ctactataag ttcctctaaa
    21781 ttatatgggg tacataaaaa tgtatttaca tatttatcat ttaaaataaa actaagtgga
    21841 tattctgcaa ttatttcttc attatctatt gatacttcat gttcatttat tttgcttact
    21901 tctacattaa aagaatttaa atatttgtag tccactagac tataactatt ttcaaaaact
    21961 ttatccttac tattttcttg taatttagtt tttgagtata tattgttcat aaaatattag
    22021 cctttctgcc ttttataaat cttaaatcaa ataaatcatt agatatttgt agctgtcatt
    22081 ttgtctttca aactttgata ctcttctctt acacattttt ctgctaattc ttggtcttca
    22141 agtttttcaa gtttaacagc acaatattta tactctggtg ttttagatat agggtctaaa
    22201 tttgctattg taagttcatt gcaagctcca acccaccact gataagtcat atatgttgca
    22261 ccttccttaa ctctatcagt aactgttgct cttgttatta cacttcctct tcttgaacta
    22321 attctaacta attcatcatc ttcaattcct aatttttcag catcattaga atttatttga
    22381 acacgtcctg gttcatcttc taatgaacta agtgttctac aattaccagt cattgttctt
    22441 actgaatagt gtccaacttc tcttacagta cataaactaa atgggtattc atcatcttca
    22501 acttccatag ggggtctcca ttcagcagcg aacaaattac ctttaccatt aggagtagaa
    22561 aacttttgac cttcatacag atacatagtt cctttatctt ccatactttc actcttacaa
    22621 ggccattgaa cgcatccttg agtttctatc ttttcataag tagcacctaa gaaacttgga
    22681 cataattgtc taagttcgtc ccatatttcc ttagtatttt tataattcat aggatatccc
    22741 attgctgtag aaatttcgct tataatttgc cagtctggtt ttacatctcc ttgtggctct
    22801 atagcttttc tcatcaattg gaatcctcta tctgcacatg tatatactcc ctcatgttct
    22861 ccccaagaag tagctggaag tataacatca gcatgaagtg ctgtcttatt cataaatata
    22921 tcttgaacaa tgacaaattc caattcatct aatgcttctc taacttctga cgcatctggg
    22981 tcactttgca ctgggtcttc tccaaatata taataagctt taagcttttt ctcttttagt
    23041 actaagtctg gaacttgagt taaactataa ccattattag gagataattt taccccccaa
    23101 gctttttcaa acttttctct tatcttatca tcagttacat tttgatagcc aggatataca
    23161 ttaggaagag ctcccatatc acaagctcct tgaacattat tttgtccacg tacaggtcct
    23221 attccaacgc tttctctacc aaaattacct gtaagtagag caattgatgc aagtccctta
    23281 actacatcaa cagcttgacc aaattgacag acacccattc cataaagtat catagctttt
    23341 ttaccttttg catattctct catagctttt cttataagct cttctgggat tcctgtaatc
    23401 ttttcagcat atttagctgt atatggttta acaatttctt tatactcatc aaatccttgt
    23461 gtatgatttt gaacaaattc tttattgtaa agaccttctt ctattaatac atttccaaaa
    23521 gcatttacta aaaccatgtt tgttccacct tttataggta gatgtatatc agcaattctt
    23581 gcagattcag ttactctagg gtcagtaaca attaatttag caccattttt tttagctttt
    23641 actattctat ttgccactat tggatgtgaa tctgctccat tatatccaaa tataaataaa
    23701 acatctgcat tttctatctc tggtatagaa ttagacattg caccactacc taatgagtaa
    23761 gccagaccgg ctacagatgg tgcatgtcag acacgagcac aatggtcaac attattagtt
    23821 cctattgtag ctctcataaa tttttgcatt atatagtttg cttcattacc aggtcctctt
    23881 gcagaacctg ttcccataat agaatcaggt ccatattttt ctttaatttc atttagtctt
    23941 gaagctgtat agtttatagc ctcatcccat tcaacctctt ctagtacacc ttgttttctt
    24001 atcattggct tttttagcct aggtgttaga attttagggt catttagaaa atcccatcca
    24061 taacgacctt ttaaacacaa actttcttga tttgttctac cattagccgg ttcagctctt
    24121 aatatcttat tatcttttac tactagatat aattggcatc cagctccaca gtatggacaa
    24181 actgttaaaa tttttttctc catacaattc cccccttaga tttatcccct gataagtaaa
    24241 atttatctta aagataatga gtgtttttat aatatgcaaa atcataaata ttttgcaata
    24301 ctatctatta aattcaacaa ttttctcaca attcctctta aatttcaaaa aaaatatgtc
    24361 ataataatct ttatatttca accatttaat taaaatattc tagttcttat gacatatttt
    24421 ttaatatact caatatatat tctttaaact tatttagttt tcacttaaaa caatatttct
    24481 gccaattcta tttttcattc tatagtgagt aattacagta aatattccac ttccaatcag
    24541 aatcaaatag tacataaatc caccatataa aaatactgca tatcccatca aaggttgagg
    24601 gaatactgat ttgaatatag tgaaaaaggc taattcatta gctcctacat tcccaggtgt
    24661 tggtattgga gatattgcca tatataaaaa tgcttgtaat gtcagtatat aaatatagct
    24721 ataaccttgt aaattaaatg ctttatatat ccaaaatgaa acactaaagt aagcacttaa
    24781 ttgaataaaa gttactatta tagttgaaat taataccttt ttattctttg caaaaaaatt
    24841 tattgcttta ctataatcat ctata
//
LOCUS       DAELLV010000031        24776 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.031, whole genome shotgun sequence.
ACCESSION   DAELLV010000031 DAELLV010000000
VERSION     DAELLV010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24776
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.031"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            2..2014
                     /locus_tag="KPD58_001090"
     CDS             2..2014
                     /locus_tag="KPD58_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG0264873.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIVLGSKSKVSVKFKEKPDADSITLKYKCYDMPLNTTLNYNQSTGAYEGTINYNQDPE
                     YLNVWELQGITINSKNNPKTLNRQDLEKLGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSSPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPKDIIIIGDENAISKTTANQIKSTVNASQTRLNGSNRY
                     ETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKDSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNGITKDSVTNNRVYGADRHETNANVIKKFY
                     TDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKDNLEAKSANKVY
                     KIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSAPGNSSGGMIEKD
                     YNLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLKPDLFTSIHYNGSTNK
                     QGHGVEVFYKLKDKNGGITKTVATNILNRILEKFKLTNRGIKTRVLPSDSTKDYLYVL
                     RSNDMPAVLVECAFLDNENDMSLINSSAKVKEMGTQIGKGIEDSLK"
     gene            2256..4169
                     /locus_tag="KPD58_001091"
     CDS             2256..4169
                     /locus_tag="KPD58_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264874.1"
                     /translation="MEYLFEKSSLKEYGNEALDKYRELLDKTGNNSRCILLLVPNNNT
                     KIRYERSLRLNYSEELKITTYISFVKKELVKYWPLIIEKCDEISTKVVSPTFISNSLS
                     EYLIVQKVKEKRIQEGYFEDITGTNKSIANSIMTNINKSAFNLIDFDFIGEKIYSSKK
                     NKDSIYRFSYTQMDEIISYYINSLLSNSILDNALSIYLYNQYLLNDDFYLKNLFTEVR
                     YIIVDSLESSSNAEVDFITEALDNTLQSYIYFDYSRDYSVFNNVDIDYINEKIISKIK
                     PKEENIKKKEIRIEDLYLLPANIELNQSSQLYNEMLDLISEKVISLIEEDVSPRDIAI
                     ISPINNSILEHQIRDSLIEKNIDVFSTKKDKKAVDYPYGNALVVATCIFYGYLDFIKD
                     DEYLSFLETLLEINRIKAFKIFNETRHLSIDNIDEHSQYREILQYIEEKKKSDIKIHE
                     FLTQFYIDKMLNLKEGKKNVGLCKKIIAESESFSESLSLLGMKKEKIFIEALKSTIND
                     YYSVVDIEELKSKDKIVITTPYSYISSNIDRSIQIWVDIGSNAWNMKIEKDISNLIVL
                     RKSFEEKKIYTNEMEEYYKKYYLYNTVYNLLLNAKKIYAFKSEYAINGYIQESILYSI
                     LLKISHKGDKNYD"
     gene            4162..6381
                     /locus_tag="KPD58_001092"
     CDS             4162..6381
                     /locus_tag="KPD58_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBG0264875.1"
                     /translation="MININYREDQIPIINYDNGTMAVPAVPGAGKTFIITNLVAKLLL
                     EQKHKGGKILILTYMNSAVNNFKGRIRKILEENKIDDTNSYEVMTIHSLAVKIIKEKP
                     EVVMLSEDFNIADDLQKSIILNECISRFRFEGGERAFRWFLKEQKDERWKEITLEAWE
                     RGFFEFAGNAISELKYKGISPDKLEEILSRGHKGMLKIILPIYKLYDRKLKQNGLLDY
                     DDILILAEKTLSLDEGLRKKFQTRYKYIFEDECQDSNEIQGNIIKIISSENKNLVRVG
                     DINQSITGTFSSSDPKFFKEFINSADFCYRMDMSNRSSKDILDLANTLVKYVTKEFRQ
                     KECRHALEDMQIKTVPNGMGYKENPNPEHYNINVKWYESWKIEIEQTARYVKGIKNKY
                     PDKSIGILVPFNEQVTQVAKELRENNLTFEELGPNSLNKRKVINNIAYIIDFMLHCDD
                     VEKLIVVLNKVFIDTDNEEEIKYFLEELKNYSTEEIIYNFEFSKLDNDLIEFKEESDI
                     YLSFKRGIDIIKQILEYPIIRLDLLVLFIGKKLNLEKEDKAVLDYISFYIKYLVSENI
                     NMDLEDVYNLLFDVKNRVFNHIIDVVYEINGYEPEPGSITLCNYHKSKGMEWDCVFLL
                     GLVEYNFPDNINQKFQSDKWYLKEKYKNPMAIIKSEVEAILKGSISTDYAHKTKIDSI
                     NEKIRLLYVGITRAKEMLVLSGSAYRDESDIGNKRKEQKPCIYLSRLNQHIIEKRSN"
     gene            6490..7320
                     /locus_tag="KPD58_001093"
     CDS             6490..7320
                     /locus_tag="KPD58_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HBG0264876.1"
                     /translation="MNDKLKYFSYSQNSINTYKSCPLKFKYKYIDRINWKNDDVGSRE
                     YYETLKTGRDFHLICERYFSNIPLGIYFNEDDKNSKKFLKWMENIKKVVPIEKGKTYL
                     PEYEVRMTLNGDIIQAKYDLVVIGNNNIEIWDWKTESKKIDYKHVENRIQTIVYMFLA
                     KEVIPKVLKMDINVKDINMRYYQPEFDDLPITILYDEEKHEANRNKIQNYISMIKNTN
                     YEEHTYEDNLYNDIEKVYNKEERMCYRNKKHCKYCEFNKLCNGKEIDYSILEAEIYGT
                     "
     gene            7310..10777
                     /gene="addB"
                     /locus_tag="KPD58_001094"
     CDS             7310..10777
                     /gene="addB"
                     /locus_tag="KPD58_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HBG0264877.1"
                     /translation="MGLRFVLGRSGSGKSTYILDEIKKEAQKNETTSIILLVPEQYTF
                     EAENRVSKLFLGKEKDKYLRVRVLSFKTLSNIVFSQVGGLTDVNINSSGKAMMVYRAI
                     EDVSEELNVFSKSKSQSGFVSSITDMISEMKQYNISPEMLENISGELDNETLSLKLKD
                     ISKIYNSFEGKLHENYVDAQDMLTSLASKIELSSYLDGACVYIDEFTGFTPNQYNVIK
                     SILNKSKSVNISLTVDDINYIGYSKSDMFSRTKFTYSKLTQLCNEEGIKILPQVNLNT
                     GVIKRFEKVKELQHLERFYNAYPYKIYSNPTENIKIKEFNNLYSEVEEIAREIVHLVR
                     DKNVRYRDITIATRDLNRYDFLVHSIFNEYNIPNFIDKKREAKSNPIVILIISALEMK
                     NRRYGYETMFRYLKSGLIGIDNDDINLLENYVLANGIKGKKWFDEKWDYRITQSLSGQ
                     ESEFELELKEKINEIKNRVLEPIVILQEKLRGKNRVKEICRYIYEFLLDINMPETIES
                     LIVNFKDKGELDVANQYSQVWDIVVDILDQMVELMGDEIISLEKFIKLITLGFDEYEL
                     GLVPPSIDQVLVSSVDRMKNPDTKYLYLVGTTDGVFPLITKDSGILSDNDRESLGNKG
                     IEVDIDSKTRSFEEQFLVYKALTSTSKNLTITYPISDHEGKTLRPSIIISRLKKIFPN
                     IENKSYLVEENKSTDKDILKKITVKSPTFNELINIIKNYDSDGYNTEEINSIWLDIYR
                     YYLKDEVYSSITKKVIKGLSYTNQVHKIEEKKIRSLYKSNSLSISRLEKYAECPFAYF
                     IQYGLKAKKRKEYSFTPPDLGTFIHNILDRFSKELLQDNLTWRDIDEKYIELKIGIIV
                     DEIILKIPGYILNSSERYKYLAYRLKNMLTTAITIISQQIKQGSFEPIDYEVKFGNNG
                     KYPPIKMVLENGQEVSLIGQIDRVDEFEEGENKYIRIIDYKSGNKSISLTEIYYGLQL
                     QLLVYLDAILESAKDEDININPAAILYCRINNPIAKFNEDKDDEEIQEAILKELRMKG
                     LVVKDSHIVKEMDKSLIDGERKNSLVIPVGLTKDGNVGKSTSAISYEDFKLLRKYVRH
                     AIKDLCEEMLSGEIRIAPYKHKDGTSCDFCDYSAICQFDSTIKDNKYKNLNNKSNEEI
                     IKMMKGDVN"
     gene            10777..14604
                     /gene="addA"
                     /locus_tag="KPD58_001095"
     CDS             10777..14604
                     /gene="addA"
                     /locus_tag="KPD58_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HBG0264878.1"
                     /translation="MSSPKWTKEQLEVIESRECNLLVAAAAGSGKTAVLVERIIQMIT
                     SRENPIDIDKLLVVTFTNAAASEMRERIGDAIGKALDENPENKHLQNQLVLLNKSSIT
                     TIHSFCLDVIKSNFHRINLDPNFRIGDQTECAILKQEAIEEVFEDLYEERDEGFLNLV
                     ESYAERGGDKEVQDIILGIYSFAMASPEPKKWLIDSAERFNIDENFDFSQSIWARAIL
                     DTVKIEINGLCLNMERALKEVESIEELETFAEKLSVEYKKIADISQACNKSWDEAYKK
                     MASMSFENYVKGVKRISKDAPSYIKESKEKAKTIRDKTKKSLESIVSATFNKDNDSIR
                     EEIKYLYNIVKPISSVVLRFEEEYSNKKREKGIIDFNDIEHFALNILTDVDEKGNIVP
                     SDIAVGYRNKFYEIFIDEYQDSNLVQEVLLKAVANTETPNRFMVGDVKQSIYRFRQAK
                     PELFLQKYNNYNDKKGSSHRKIMLYKNFRSREEVVDAVNYIFENIMNENIGEIEYTEK
                     ERLNLGANFNVDTDEKSIIGGATEIHLIQKDNKLDDDIINDKDDRINNKENEIEEEEN
                     LDNIQLEARMVGNIIKDLMKVNEDGKIQKVYDKGIDGYRPVEFRDIVILLRATSAWAP
                     VFADELMNMDIPTYADVGVGYFDTIEIKTILSLLQIIDNPMQDIPLISVLKSPIFGFT
                     PEDLIDIRVQSKDKIFYEVLKSTAEYDGFTDSQNENESEFIPSEECINKSKDFLIKLK
                     EFKEKSMYMSTDEFIWYLYTRTGYYAYVGALPGGSQRQANLKVLFERAKQFEETSLKG
                     IFNFVNFIEKLKKSSSDMGSAKTLGENANVVRIMSIHKSKGLEFPVVICSAMGKNFNT
                     QDFKKSILYHHNLGYGPQFVDYERRISFPSIAKEALKSKINIENLSEEMRVLYVAFTR
                     AKEKLIITGSTRNIQDSIKRWSNGIESLDTISQYEILKGKNFLDWIMPCVLRHRDLSN
                     LLEEVGLDAVFNVEHNSKWYGKLWNKNDILVEKKSDEEKESIEEILEKIDVDNPDSDY
                     YSEIEEKLNYIYPYEFSTRKPATISVTEIKKIQNNYEEELINTIFEQKVILKKPLFIQ
                     NEEEREKISGTERGTIVHLVMEVLDLKNVSSVNDIKSQIRGFVSKGIITEKQASIVNP
                     YKIYKFFASNIGKRMLNAEIINREKSIYAQVNMKDIYIYEKLINNDDKKLYDNESVML
                     RGIVDAYFEEDNQIVLVDYKTDFVNEENINQIIEKYKKQLDLYADIIETLTGKSVKEK
                     CIYLFGVDEAVCY"
     gene            14650..15861
                     /locus_tag="KPD58_001096"
     CDS             14650..15861
                     /locus_tag="KPD58_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HBG0264879.1"
                     /translation="MRFIHTSDWHLGKSLEGHSRIEEQAKFCEEFIKIVESNEIDMVI
                     IAGDVYDTSNPPAQAEKLFYQTVSRLANNGQRCVLIISGNHDNPERLSAITPLAHEQG
                     ILIYGYPLSATIEAKYKGFEITYATQGCTKLNINGENIVVATLPYPSEKRLNEVFSSE
                     DEFEKQKNYSEKVGDIFRSLEENFRSDTINIAVSHIFVIGGESTESERPIQLGGSFLV
                     ERKDLPEKAQYTALGHLHKQQKASERLNAYYSGSPLQYSKDERAYTKGAYIVDIKAGE
                     KPIIEDVYFNNYKPIEVFKCNGIEEALDICEQNQDREIWSYFEINTDEIISQNEIKKM
                     KELLKDIIEIKPIITSCYEQESVDIKEKSMAELFREFYSFSKGVEPKGELMDLFLDII
                     SEEGESADETN"
     gene            15848..19375
                     /locus_tag="KPD58_001097"
     CDS             15848..19375
                     /locus_tag="KPD58_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0264880.1"
                     /translation="MRPIRLELTGLNSYIDKQVIDFEKLIERGLFGIFGTTGSGKSTI
                     LDAITIAMYGNISRNTKEYINSVCDKAIISYEFEIGSKNTRRRYIVDRTIARSKTGTK
                     TSNARLIEVLNDNTQNVLADKVVEVNEKVAQVVGLTANDFTRSVVLPQDKFNEFLRLS
                     RADRRDMLERIFNLEKYGRSLGEKVKKRKNIQLQNLKDLKSKLSQYDGITEEVYNKIN
                     QELIELKNLEKDKNNALDLAQKSYEESKTVYEEQLKLEKNELRKNELDLKNSEIKEKI
                     ALVENDSNAKKVDPYISSVQNLEKKLDEDSFAVSNLEKKLAILNQELEVTKNRYEKIN
                     KIKNEEVPKLSEEKIRLQQAIKLEEELVLLDRELKELKESGINLNKTKVELEKVKQVS
                     ESRKDAVTKSIKEVEGKIDKVNISAELKQKIFLAYEYEKDYNNVLEEKNQKLNKLEEI
                     LKETENINLKVRYIDKDKNDVNRNLENLSLHLDVLLKKCPGKSADLLLKSEYVTELRN
                     KANNTKENEIKKSSIQDELKIILESKFNTEREVNLLNEKLENNRKNRDDLEKELEELK
                     YLNLASELRRELKENMPCPVCGSKHHENHDITKYDENISFVKEKLEKLEKEKISIRNN
                     IEELNAKVSGYLSIEKMKTKELEDVKGKLGGIPSSQLLKKLDEEQRKLALLKSNIQEW
                     EKEKESTENKVTLAKEEKNKIEKEELKIRESLNNYKKLTRDLNIEIEGLENKCKKIKQ
                     EYLGLKTITKVSNLLSKVKEIRENEKELELLSTGHSNLLKNRDSLDIKIRENESKLHE
                     IEIELIKARELYVEKKLSRDNKYKEVISITKGDLAKNLLHNVEENICKILEQEESSKK
                     KLEEQRLEYEKNIAEKHNIDGRLKTAKEQYKDQKSTLNKLLAENKFESIYAVKRALLD
                     TDAVKKLIEEILEHEEEQKLLSFKIKSSKEKLNGRSIKKEYFEQLKDEIYNLKVEIGN
                     ISKDIGANQNQLITLKDSLDKINDFNKQLKVVEHNVDLLEELDKCVQGNKFVEYVSTN
                     QLKYIALEASKRLDGITKGRYALEIDSTLNFVMRDNFNGGERRSIDTLSGGETFLTSL
                     SLALALSSQIQLKGSAPLEFFFLDEGFGSLDSELLEIVIESLERLHSNNLSVGIISHV
                     EELKNRVPVKLLVSSSEAGIGSKVKIEYS"
     gene            19506..20759
                     /locus_tag="KPD58_001098"
     CDS             19506..20759
                     /locus_tag="KPD58_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBG0264881.1"
                     /translation="MLESPNNLIQIIFLIVLLIGSAFFSASETALMSLSKIRIRYMQD
                     EGVKGAKLVSSLIENPNKLLSSILVGNNVVNIAATSISTSLFIGLMGEKGVALATAVM
                     TVLVLIFGEITPKTIAANNSEKVSLLVSKPIKAIIFILRPIVWIFNIITNIIFKLFGI
                     TNKGAKSFITEEELKTMVNVSHEEGVLEMEEREIINNVFEFGDMQAKNAMVQRIDMVA
                     IDMEDSYDEIIQVFKTEKLSRMPVYEETIDDIVGILNIKDIIFLSDEEIESFDIKNYM
                     REPFFTYEFKKITQLLEEMKLEKSQMAIVVDEYGGTSGLLTIEDLVEVIVGDIEDEYD
                     EEEDEIQVIKEDEYIVDGSTKIGDVNELIGVNLESEEFDSIGGFIIGHLSRLPEENEV
                     IEVDNIRFCIESIEKNRIKKIRIYT"
     gene            20908..21960
                     /gene="ytvI"
                     /locus_tag="KPD58_001099"
     CDS             20908..21960
                     /gene="ytvI"
                     /locus_tag="KPD58_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HBG0264882.1"
                     /translation="MSILENNFLYKLKNNTIFFCIYTLLFIFIYKAFPYIAPFFLGAI
                     IALMINPISQKLENKFRINKGISTLVLSFLAVAIVSTVTTIIVINSMKELMGFLNNIS
                     ANPEDISNTIMYFLNKINDFMKSFQEIANFDVEQLVNKFSGEVMQITKNLLTSILGLA
                     TSIPYIIIFIITLFIATYFIAKDLDKIENSFYNMFTVDVRKKVKNVKKEAGLSLVGYI
                     RAYTILMAITFFAIWGSFALFGLKYGLIVGFVGALMDLIPFLGIITIYLPVIVYYFLI
                     KNYFIAISMTVIFFVLSLIREILEPKLVSVNVGLNPLATLAAIFIGIQVKGIIGVIFC
                     LGLVCMHDILKKVDIF"
     gene            22051..23277
                     /gene="pepT"
                     /locus_tag="KPD58_001100"
     CDS             22051..23277
                     /gene="pepT"
                     /locus_tag="KPD58_001100"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBG0264883.1"
                     /translation="MKQKVVERFLKYVSFDTTSNSQCENCPSSEGQRVLAKYIVEELK
                     TMGVDDVSLDENSYIMATLKGNTDGVDTIGFISHLDTIEDVSGKDIKPRIIENYDGKD
                     IVLNEALNVITYVKDSPELEEFKGDDLIVTDGTTLLGSDDKAGIAEIVTAIEYLINHP
                     EIKHGDIKIGFTPDEEIGRGADLFDVEKFGAKYAYTLDGGIVGELECENFNAANATIT
                     IHGRNVHPGSAKNKMVNAIHIAAEISEMFPADERPETTEGYEGFWHLNSIGGNVENVS
                     MAYIIRDHCKEKFENRKSIMIENIEKINKKYDNRVELDLKDSYYNMKEKIEPVMFIVD
                     IAKEAMEELGIKPRLVPVRGGTDGARLSFNGLPCPNIFTGGLNFHGKNECIPVSSMEK
                     ATKLIVRIAEKYAERV"
     gene            23503..24555
                     /locus_tag="KPD58_001101"
     CDS             23503..24555
                     /locus_tag="KPD58_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp18"
                     /protein_id="HBG0264884.1"
                     /translation="MKIKSKKIIAFALSLGIMLPSCVYADTISKNQNAQVDMNNYLQG
                     RKSPKEEKLIGEDRFDTAIKISQSGWNNGSERVFLVNSNSLPDALASTPLASKLDAPI
                     LLTNKNSIPYNVTEEIKRLNPAEVILIGSEGAISSSVKESLEDMEVLVSRIGGADREE
                     TSLLLTRQLDDTGDLGVSKVAVVNGYNGLADATSISSPAASDNTAIIYTGKDSIRSEA
                     KNFITQNSTKTYIVGGEYSISKKLEGQLVNSERLAGTDRKDTNAKVLEKFYGKSSKVN
                     NMYFAKDGSGREADLVDGLAAGVLASKTKSPVVLASGSLSSAQKSFIKKVKADKFVQV
                     GGGKNSKPYAEALALQ"
BASE COUNT        10577 a         2380 c         4408 g         7411 t
ORIGIN      
        1 tatgagaaga aatacaaaat tattaacaac agggattctt tcaatggcaa tcgtcgcacc
       61 tacaatggca tttgctactg aatctaatgc tatggaaaat aacgctgatt taaatataaa
      121 cttagagaaa aaaagtatcg ttttaggtag caaatcaaaa gttagtgtca aatttaaaga
      181 aaaaccagat gcagatagca ttacattaaa gtataaatgc tatgacatgc cattgaatac
      241 aactctaaat tacaatcaat caactggggc atatgaagga actatcaatt ataaccaaga
      301 cccagaatat ctaaatgttt gggaactaca agggataaca ataaacagca aaaataatcc
      361 taaaacttta aacagacaag acctagaaaa gctgggatta aatttaaaag actataatgt
      421 aacacaggaa tgtataattg aagatataac ttctagaaaa gatgtaaata aatatttgag
      481 aaaaacttct tcacctatta cagaacttac aggaagtgat agatatgaaa cagcagttaa
      541 aataagtaaa gagggctgga aaaatggttc agataaggta gttataataa atggggatgt
      601 aagtatagat ggcattatat caactccact ggcaaccaca tataatgcac caatactttt
      661 ggttgaaaaa aacaatgtac ctaatagtgt aaaatcagaa ttaaagcgcc taaaccctaa
      721 agatataatt ataattggag atgagaatgc tatttctaaa actactgcta atcaaattaa
      781 atcaactgta aatgctagtc aaacacgttt aaatggttct aatagatatg agacatcttt
      841 attgatagca aaggaaatag ataaaaatca tgatgtggaa aaagtataca taacaaatgc
      901 taatggcgga gaagtggatg cacttactat agcagcaaaa gcaggtcaag acaagcaacc
      961 aattatatta actgataaag atagtattac agacaataca tataaatggt taaagagtga
     1021 ggatttacaa aatgcttatt ttataggtgg tcctcaaatg atatcaacaa atgttataaa
     1081 taaggtaaat ggaataacta aagatagtgt tactaataat agagtatacg gagcagatag
     1141 acacgaaaca aatgcaaacg taataaaaaa attctataca gatgatgagt tagaggctgt
     1201 tttagtagct aaatcagatg tacttgttga tgctttagca gcaggtccat tggctgcgaa
     1261 cttaaaatct ccaatactta taacaccaaa gacgtatgta tctgcatacc ataaagataa
     1321 tttagaagct aaatcagcta ataaggtata caaaatagga ggaggattga cttctaaggt
     1381 aatgagctct atagcatcat cattatctaa acacaatacg actccaacag aaccaggaaa
     1441 tagtggtggc aagacagtta tgattgaccc agggcatggt ggttcagcac ctggaaattc
     1501 atctggagga atgattgaaa aagattacaa tttaaatact tcacttgcaa caactgaata
     1561 tttacgttca aagggattca atgtaataat gacaagagac acagataaga ctttatctct
     1621 tggaaataga actgctctat ctaattcatt gaaaccagat ttatttacaa gtatacatta
     1681 taatggctca actaataaac aaggtcatgg tgtagaagta ttttataagc ttaaagataa
     1741 aaatggaggg attactaaaa ctgtagctac caatatatta aatagaattt tagagaaatt
     1801 taaacttaca aatagaggta taaaaacaag agtacttcct agtgattcta caaaagatta
     1861 tttatacgtt ttaagaagta atgatatgcc agctgtactt gtagaatgtg catttttgga
     1921 taatgaaaat gatatgagtt taataaactc atctgcaaaa gtaaaagaaa tgggtacaca
     1981 aataggtaaa ggaatagaag attcattaaa ataaatggaa ataaaaatat ttaaaaactt
     2041 aatgtaaatt tatagttgta aatttatact aaagtcaaaa aatgaaatat aagtgtatca
     2101 atagtaagac tgtatgtaca tttgcacatg tgaatgcaag tatatataca gtcttttttt
     2161 gtagttaaat gcaataattt gattgtgttt tgtaaaatac acaatatgat ataattagat
     2221 aatatacact taatttaatt gaggaaggtg attatatgga atatttattt gaaaaaagta
     2281 gtttaaaaga atatggaaat gaagctttag ataaatatag agaattatta gataaaactg
     2341 gtaataattc aaggtgtatt cttttgttag taccaaacaa taacacaaag atacgatatg
     2401 aaaggtctct aagattaaat tatagtgaag aactgaaaat aacaacatat ataagttttg
     2461 tgaagaaaga attagttaaa tactggccac taataataga aaaatgtgat gaaataagta
     2521 caaaggttgt atctccaaca tttatatcaa atagtttaag tgaatattta atagtgcaaa
     2581 aagttaagga aaaaaggata caagaaggat attttgaaga tattacagga accaataaat
     2641 ccatagcaaa tagtataatg actaacataa ataagtctgc atttaattta attgattttg
     2701 attttattgg agaaaaaata tattcatcaa agaaaaataa agatagtata tatagatttt
     2761 catacactca aatggatgaa attatatcat attatataaa ttccttacta tcaaattcaa
     2821 ttttagataa tgcattaagc atatatttat acaatcaata cctacttaat gatgattttt
     2881 atttaaaaaa cttatttaca gaagtgagat atataattgt ggatagcctt gaaagctcaa
     2941 gtaatgcaga agttgatttt ataacagaag cattggataa tacattacaa agttatatat
     3001 attttgatta tagtagggat tattctgtat ttaataatgt ggatatagac tatataaatg
     3061 agaaaataat atcaaaaatt aagcctaaag aagaaaatat taaaaagaag gagattagaa
     3121 tagaagattt atacttatta cctgcaaata tagaattaaa tcaatcaagc caattatata
     3181 atgaaatgtt agatttaata agtgaaaaag ttatatctct tatagaagaa gatgtatctc
     3241 caagagatat agcaattata tctcctataa acaatagtat attggagcat caaattagag
     3301 attctttgat agaaaaaaat atagatgttt ttagtactaa gaaggataaa aaagctgtgg
     3361 attatccata tgggaatgca ttggtagtgg ctacatgtat attttatgga tatttagatt
     3421 ttataaaaga tgatgaatac ttaagctttt tagaaactct tttagaaata aataggataa
     3481 aagcatttaa gatttttaat gaaacaagac atttaagtat tgataatata gatgaacata
     3541 gtcagtatcg tgagatttta cagtatattg aagaaaaaaa gaagagtgat ataaaaatac
     3601 atgaattttt aactcaattt tacatagata aaatgttaaa tttaaaagaa ggtaaaaaga
     3661 atgttgggtt atgtaaaaag ataatagctg aaagtgagtc ttttagtgaa agtttaagtc
     3721 tacttgggat gaaaaaagaa aagatattta tagaagcttt aaaaagtaca ataaacgatt
     3781 attactctgt tgtcgatata gaagagttga aatccaaaga caaaatagta ataactactc
     3841 cttattcata tatatcatca aatatagata gaagtataca aatatgggta gatataggaa
     3901 gtaatgcttg gaatatgaaa atagaaaaag atataagtaa tttaattgta cttagaaaat
     3961 cttttgaaga aaaaaagatt tatacaaatg aaatggaaga atattataaa aaatactatc
     4021 tatacaatac agtatataat ttgttattaa atgcaaagaa aatctatgca tttaaaagtg
     4081 aatatgcgat taatggttat atccaagaga gtatattata tagtatactt ttaaaaatct
     4141 cacataaagg agataaaaat tatgattaat ataaattata gagaagacca gataccaata
     4201 ataaattacg ataatggtac tatggcagta ccagcagtac caggtgctgg aaaaacattt
     4261 ataataacaa atttagtagc taaactatta cttgagcaga aacataaggg tggtaaaata
     4321 ttaattctta catatatgaa tagtgctgta aataatttta aaggtagaat aagaaagatt
     4381 cttgaagaga ataaaataga tgatactaat tcgtatgaag taatgactat acacagtttg
     4441 gctgtaaaaa taataaagga aaagccagaa gttgtaatgt taagtgagga ttttaacata
     4501 gctgacgatt tgcaaaaaag tattatatta aatgaatgta taagtagatt tagatttgaa
     4561 ggtggagaaa gagcttttag atggttttta aaggagcaaa aagatgaacg ctggaaagaa
     4621 ataactcttg aagcctggga aagaggtttt tttgaatttg caggaaatgc aataagtgaa
     4681 cttaagtata agggaatttc tccagataaa cttgaagaaa tattgtcaag aggacacaaa
     4741 ggaatgctta aaattatttt accaatatat aaattgtatg atagaaaatt aaagcaaaat
     4801 ggattgctag attatgatga tatattaatt ttagcagaaa aaactttaag cttagacgaa
     4861 gggcttagaa agaagtttca aactagatac aaatatatat ttgaagatga gtgccaggac
     4921 tcaaatgaaa ttcaaggtaa tataataaaa attatatcta gcgaaaataa aaacctagtt
     4981 agagttggag atataaatca gagtataaca ggaacatttt catcatcaga ccctaaattt
     5041 tttaaagaat ttataaatag tgcagatttc tgttatagga tggatatgtc aaatcgaagt
     5101 tcaaaagata tacttgattt agcaaacact cttgtcaagt atgttacaaa ggaatttaga
     5161 cagaaagaat gtagacatgc tcttgaagat atgcaaataa aaactgtgcc aaatggaatg
     5221 ggatataaag aaaaccctaa tccagaacat tataatataa atgtaaagtg gtatgagagt
     5281 tggaaaattg aaatagagca aactgctaga tacgttaagg gtataaaaaa taaatatcca
     5341 gataaaagta taggtatatt ggttccattt aatgaacaag tgacacaagt tgctaaagaa
     5401 cttagagaga ataatctaac ctttgaagaa ttaggaccaa attctttgaa taaaaggaag
     5461 gttataaaca atatagcata tattattgat tttatgttgc attgtgatga tgtagaaaag
     5521 ttaatagttg tacttaataa agtttttata gatactgata atgaagaaga aataaagtat
     5581 tttttagaag aattaaaaaa ctattctacc gaagaaatta tttataattt tgaatttagt
     5641 aagttagata atgatttaat agaatttaaa gaagaaagtg atatatactt aagttttaaa
     5701 agaggaatag atattataaa acagatatta gaatatccaa taataaggtt agacctctta
     5761 gttttgttta ttgggaaaaa acttaattta gaaaaagaag ataaagctgt actggattat
     5821 atatcttttt atataaagta tctagtatca gaaaatataa atatggattt agaagatgta
     5881 tataatttat tgtttgatgt aaagaacagg gtgtttaatc atataataga tgtggtatat
     5941 gagataaatg gatatgaacc agagcccggt agtataacac tatgtaacta tcataagtca
     6001 aaggggatgg aatgggattg tgtgttttta ctaggtcttg ttgaatataa tttccctgac
     6061 aatattaatc aaaagtttca gagtgataaa tggtatctaa aagaaaaata taaaaaccct
     6121 atggcaatta ttaaatcaga agttgaagct atattaaaag gaagtatttc aacagattat
     6181 gctcataaaa cgaagattga ttctataaat gaaaagatta gacttttata tgtagggatt
     6241 actagagcaa aggaaatgtt agtactttca ggaagtgctt atagagatga aagtgatata
     6301 gggaataaga gaaaagagca aaaaccatgt atatatttaa gcagacttaa tcaacatatt
     6361 attgaaaaaa ggtcaaatta atatataata agttattaaa agattgcttg aaatatatct
     6421 aaaaggggat aaaacaatat gtacagtaag taactactaa aaaataaagt taagagagaa
     6481 gtgatatgag tgaacgataa attaaaatat ttttcttata gtcaaaattc aattaacaca
     6541 tataaatcat gtccattgaa atttaaatat aaatatattg atagaataaa ttggaaaaat
     6601 gatgatgttg gtagtagaga gtattatgaa actttaaaaa caggaagaga ttttcatttg
     6661 atttgtgaaa gatattttag caatatacct cttggaatct attttaatga ggatgataaa
     6721 aatagtaaaa agtttttaaa gtggatggaa aatataaaaa aagttgttcc tattgaaaaa
     6781 ggaaaaacgt atttgccaga atatgaagta aggatgacat taaatgggga tataatacag
     6841 gcaaagtatg atttagttgt aattggaaat aacaatatag aaatatggga ttggaaaacc
     6901 gagtctaaaa agattgacta taaacacgta gaaaatagaa tacaaactat tgtttatatg
     6961 tttttagcaa aagaagtaat acctaaagtt ttaaaaatgg acataaatgt taaagatata
     7021 aatatgagat attatcaacc tgaatttgat gacttaccaa ttaccatttt atatgatgaa
     7081 gaaaaacatg aagcaaatag aaataaaatt caaaattata taagtatgat aaaaaacact
     7141 aattatgaag aacatacata cgaagacaat ctatacaatg atatagaaaa ggtatataat
     7201 aaagaagaac gtatgtgcta tagaaataaa aagcattgca agtattgtga atttaataag
     7261 ttatgtaatg gcaaagaaat agattatagt attttggagg cggaaattta tgggacttag
     7321 gtttgtatta ggtagaagtg gaagtggaaa atccacctat atactagatg agattaaaaa
     7381 agaagctcaa aaaaatgaaa cgacatcaat tatactttta gttccagaac agtacacatt
     7441 tgaggctgaa aatagagtaa gtaaactatt tttaggaaaa gaaaaagata agtatctaag
     7501 agttagggta cttagcttta agacactaag taatatagtt ttttctcaag taggaggatt
     7561 gacagatgta aatataaatt ctagtggaaa agcaatgatg gtatatagag ctattgaaga
     7621 tgtaagtgaa gaattaaatg tgttttcaaa atcaaaatcg cagtctggtt ttgtaagttc
     7681 aattacagat atgatatcag aaatgaaaca atacaatata agccctgaaa tgttagaaaa
     7741 tatatctgga gaattggata atgaaacttt aagtttaaaa ttaaaagata taagtaaaat
     7801 atataattct tttgaaggga aattgcatga aaattatgta gatgctcaag atatgttaac
     7861 ttcattggca agtaaaattg aattaagcag ttatttagat ggagcttgtg tttatataga
     7921 tgaatttaca ggttttacac caaatcagta caatgtcata aagagtatat taaataagtc
     7981 taaaagtgtt aatatctctt tgactgtaga tgatatcaac tatattgggt acagtaaatc
     8041 tgatatgttt tctagaacga aatttactta ttcaaaattg actcaattgt gcaacgaaga
     8101 aggcataaaa atattgccac aagtaaattt aaacacaggt gttataaaaa gatttgaaaa
     8161 agttaaggaa ttacagcact tagaaagatt ttataatgct tatccatata aaatatattc
     8221 aaatccaact gaaaatataa agataaaaga gtttaacaat ttatattcag aggtggaaga
     8281 gatagccaga gaaattgtac atttagttag agataagaat gtcagatata gagatataac
     8341 cattgctaca agagatttaa atagatatga ttttttagta cactctatat ttaatgaata
     8401 taatatacca aactttatag ataagaagag agaggcaaaa agtaatccaa tagttatttt
     8461 aattatttca gctttagaaa tgaagaatag acgatatggg tatgaaacta tgtttaggta
     8521 tttaaaaagt ggacttatag gaattgataa tgatgatata aatttactag aaaattatgt
     8581 gttggctaat gggattaaag gtaaaaaatg gtttgatgaa aaatgggatt atagaataac
     8641 tcaaagtctt tcaggtcaag aaagtgaatt tgaactggaa ttaaaagaga aaataaatga
     8701 aataaaaaat agagtattag agcctatagt aattttacaa gaaaaattaa gaggaaaaaa
     8761 tagagtaaaa gaaatctgta gatatatata tgaattttta ttagatataa atatgccaga
     8821 aactatagaa agtttaatag taaattttaa ggataaaggg gaacttgatg ttgcaaatca
     8881 atactcacaa gtttgggaca tagtagtgga catattagac caaatggtgg aacttatggg
     8941 tgatgaaata atttctttag aaaaatttat aaaattaata accttaggtt ttgatgaata
     9001 tgaattagga ctggttcctc ctagtataga ccaggtactt gtaagtagtg tggatagaat
     9061 gaaaaatcca gacacgaaat atttatacct agttggaact actgatggtg tgtttccgct
     9121 gattacaaag gatagtggaa tattgagtga taatgatagg gaaagtcttg gaaacaaagg
     9181 aatcgaagtt gatattgata gtaagactag gagttttgaa gaacagtttc ttgtatataa
     9241 agctcttact tcgacatcta agaatttaac cataacatat ccaatatcag accatgaagg
     9301 taaaacgctt agaccatcaa taattatatc aagacttaag aagatatttc caaatataga
     9361 aaataaaagt tatttagtgg aagaaaataa aagcactgat aaagatatac ttaaaaaaat
     9421 aacggttaaa tcccctacat ttaatgaact tataaacata ataaaaaatt atgacagtga
     9481 tggttacaat acagaagaga ttaatagtat ttggttagat atatataggt actatttaaa
     9541 agatgaagtc tatagttcta taacaaaaaa agttataaaa ggtcttagtt atactaatca
     9601 agttcataag attgaagaga aaaaaataag gtcattgtat aaaagtaatt ctcttagcat
     9661 atctaggctt gaaaaatatg ctgaatgtcc atttgcttat tttatacaat atggtctaaa
     9721 agcaaaaaag cgtaaagagt attcatttac accaccagat ttaggaactt ttattcataa
     9781 tattttagat aggttttcaa aagaactttt acaagataat ttaacttgga gagatattga
     9841 tgaaaaatat atagaattaa agataggtat aatagttgat gaaataattt taaagatacc
     9901 aggatatata ttaaatagtt ctgagaggta taaatacctt gcatatagat taaagaatat
     9961 gttgacaact gctataacta taattagtca gcaaataaaa caaggttcat ttgaaccaat
    10021 agattatgaa gttaaatttg gcaacaatgg aaaatatcca cctataaaaa tggtgttaga
    10081 aaatggacaa gaagtaagtt taattggaca aatagataga gttgatgaat ttgaagaagg
    10141 agaaaataaa tatataagaa taatagatta taagtcaggc aataagtcca taagtctaac
    10201 agaaatatat tatggtctac aattacaatt acttgtgtat ttagatgcaa tccttgaaag
    10261 tgcaaaggat gaagatataa atataaatcc agctgcaata ctatattgta gaataaataa
    10321 tccaatagca aaatttaatg aagataaaga tgatgaagaa atacaagaag caatattaaa
    10381 agaacttaga atgaaggggc ttgtagtaaa agactctcac atagttaaag agatggataa
    10441 atcattaata gatggagaaa ggaaaaactc tttagtaata ccagtaggtc ttactaagga
    10501 tggaaatgta ggtaaaagta cttcagcaat aagctatgag gatttcaaac tgcttagaaa
    10561 atatgtaagg catgctataa aggatttatg tgaagaaatg ttaagtggag aaatacgtat
    10621 agctccatat aaacataaag atggaacatc atgtgacttt tgtgattact ctgctatatg
    10681 tcagtttgat tcaactataa aagataacaa gtataaaaat ttaaataata agagtaatga
    10741 agagataata aaaatgatga aaggagatgt taattagtga gttctcctaa atggacaaaa
    10801 gagcaattag aagttataga aagtagagaa tgcaatttat tggttgctgc tgctgctggt
    10861 tcaggaaaaa cagccgttct agttgagcgt ataattcaaa tgataacaag tagagaaaat
    10921 ccaattgata tagataagtt attagttgtt acatttacta atgctgctgc gtcagaaatg
    10981 agagagagga taggggatgc tataggaaaa gccttagatg aaaacccaga aaataaacat
    11041 ttacaaaacc aacttgtttt attaaataaa tctagtatta caacaataca ctcattctgt
    11101 ttggatgtta taaaatcaaa ctttcataga ataaacttag accctaattt cagaatagga
    11161 gaccaaactg aatgtgctat tttgaaacaa gaagcaatag aggaagtatt tgaagattta
    11221 tatgaagaaa gagatgaggg gtttttaaat ttagttgaaa gttatgcaga aagaggagga
    11281 gataaagaag tacaagatat tatcttggga atatattcat ttgcaatggc ttctcctgaa
    11341 cctaaaaaat ggttgattga ctcagcagaa cgatttaata ttgatgaaaa ttttgatttt
    11401 tcacagtcta tttgggctag agctatatta gacactgtaa aaatagagat taatggtctt
    11461 tgtctaaata tggaaagggc attaaaagaa gtggaatcaa tagaagaatt agaaactttt
    11521 gcagaaaagt tatctgttga atataaaaaa attgctgaca tttcacaagc atgtaataaa
    11581 tcttgggatg aggcatataa aaagatggca agtatgtcct ttgaaaatta tgtaaaagga
    11641 gtaaaaagaa tttcaaaaga tgctccttcg tatataaaag aatcaaaaga aaaggcaaaa
    11701 actataagag ataagactaa gaagtcttta gaaagtatag taagtgctac attcaataaa
    11761 gataatgatt ctattagaga agaaataaaa tatctttata acatagttaa accaatatct
    11821 agtgttgtac tgagatttga agaggaatat agcaataaga aaagagaaaa aggaataata
    11881 gattttaacg atatagagca ttttgcatta aatatactta cagatgtaga tgaaaaaggt
    11941 aatattgtac cttctgatat agcagttgga tatagaaaca agttttatga aatatttata
    12001 gatgaatatc aggatagtaa tctagtgcag gaagtactgc ttaaagctgt tgcaaataca
    12061 gaaactccaa atagatttat ggttggtgat gtaaaacaga gtatatatag atttagacaa
    12121 gcaaagccag aattattttt gcaaaaatat aataactata atgataaaaa gggaagttcc
    12181 catagaaaaa taatgcttta caaaaatttt agaagtagag aagaagttgt agatgctgta
    12241 aattatatat ttgaaaatat aatgaatgag aatataggag aaattgaata tacagaaaaa
    12301 gaaagactaa atttaggtgc aaattttaat gtggatacag acgagaaatc tattattggt
    12361 ggagcaacgg agatacacct aatacaaaaa gataataaac ttgatgatga tattataaat
    12421 gataaagacg atagaattaa taacaaagaa aatgaaatag aggaagaaga aaatttagat
    12481 aatatacaac ttgaggctag aatggttgga aacattatca aagatttaat gaaagtcaat
    12541 gaagatggaa aaattcaaaa ggtctatgat aagggaatag atggatatag acctgtggaa
    12601 tttagagata tagttattct tttaagagct acatctgcat gggctccagt gtttgcagat
    12661 gaattgatga atatggatat accaacgtat gctgatgttg gagtggggta ttttgatacg
    12721 attgaaataa aaacaatatt gtcattactt caaataatag ataatccaat gcaagatata
    12781 cctcttatat ctgtattaaa atcacctata tttggattta caccagagga tttgattgat
    12841 attagagttc agagtaaaga taaaattttt tatgaggttt taaaaagtac agcagagtat
    12901 gatggattta cagattctca aaatgaaaat gagtctgagt ttataccaag tgaagagtgc
    12961 ataaacaaaa gtaaagattt tttaattaag ctaaaggaat ttaaagaaaa gtcaatgtac
    13021 atgagtacag atgaatttat atggtacttg tacacaagga ctggatacta tgcctatgtt
    13081 ggagcattgc caggtggaag tcaaagacaa gctaatttaa aagtattgtt tgagagagca
    13141 aaacaatttg aagaaaccag tcttaaagga atattcaact ttgtaaattt tatagagaaa
    13201 ttaaaaaaat ctagttctga tatgggaagt gcaaaaacac ttggtgagaa tgcaaatgtt
    13261 gttagaataa tgagtattca taaaagtaaa ggtctagaat ttccagtagt catatgctct
    13321 gcaatgggta aaaatttcaa tactcaagat tttaaaaaga gtattttata tcatcataat
    13381 ttagggtatg gacctcaatt tgtagactat gagagaagga tttcttttcc aagtatagcc
    13441 aaagaagcat taaaaagcaa aataaatata gaaaatttat cagaagagat gagagtttta
    13501 tatgttgcat ttacaagagc taaagaaaaa ttgattataa ctggttctac tagaaatata
    13561 caagatagta taaagagatg gtcaaatgga attgaaagtt tagatacaat atcacaatac
    13621 gagatattaa aaggtaaaaa ttttttagat tggataatgc cttgtgtatt aagacatagg
    13681 gatttatcta atctactaga agaagtagga ttagacgcag tttttaatgt tgaacataat
    13741 tcaaaatggt atggaaagtt gtggaataag aatgatattt tagtggaaaa gaagagtgat
    13801 gaagaaaaag aaagtattga agaaattctt gaaaaaatag atgtagataa tcctgacagt
    13861 gattattaca gtgaaataga agaaaaatta aactatatat atccatatga atttagtaca
    13921 agaaaacctg ctactatatc tgttacagaa ataaaaaaga tacaaaataa ttatgaagaa
    13981 gagttaataa atactatatt tgaacaaaaa gtaatattaa aaaagcctct atttattcaa
    14041 aatgaagaag aaagagaaaa aataagtgga acagaaagag gtacaatagt ccatttagtt
    14101 atggaggttt tagacttaaa aaatgtaagt agtgtaaatg atataaaatc acaaataaga
    14161 ggttttgttt caaaaggtat aataacagaa aaacaagcta gtatagtaaa tccatataaa
    14221 atatataaat tttttgcctc taacatagga aaaagaatgt tgaatgctga aattataaat
    14281 agagaaaaat caatttatgc acaagtaaat atgaaagaca tatatattta tgaaaagctt
    14341 ataaacaatg acgataaaaa actttatgat aatgaaagtg tcatgttaag aggtatagtt
    14401 gatgcttatt ttgaagaaga taatcaaata gttttggtgg attataaaac agattttgta
    14461 aatgaggaaa atataaatca aataatagag aaatataaaa aacagttgga tttatatgca
    14521 gatattattg aaactttaac tggtaagtca gtaaaagaga aatgtatata tttatttgga
    14581 gttgatgaag ctgtttgtta ttagttttta tatataaatt aaacctaaaa gatagaggaa
    14641 ggggaaaaaa tgagatttat tcatacatca gattggcatc tagggaagtc cctagaaggc
    14701 cattctagaa ttgaggaaca agcaaagttt tgtgaggaat ttataaaaat agtagaatca
    14761 aatgaaatag atatggttat aattgctgga gatgtatatg atacttctaa tccaccagca
    14821 caagcagaaa aactatttta tcagactgta tcaagacttg ccaataatgg tcagaggtgt
    14881 gtattaataa tttcaggaaa tcatgataat ccagagagac tctctgcaat aacacctcta
    14941 gcacatgaac aggggatact tatatatggt tatccattaa gtgctacgat agaagctaag
    15001 tataaagggt ttgaaataac atatgcaact cagggatgta caaaactgaa tataaatgga
    15061 gaaaatattg tggttgctac attaccttat ccaagtgaaa aaagattaaa tgaagttttt
    15121 tcaagtgaag atgagtttga gaaacaaaaa aattattctg aaaaggtagg agatatattt
    15181 agaagtttag aagaaaattt tagaagtgat actataaata tagccgtatc acatatattt
    15241 gtaataggtg gagagagtac tgaatcagaa agacctatac agttaggtgg aagtttttta
    15301 gttgaaagaa aagatttgcc agaaaaggct caatatactg cactaggtca tttacataaa
    15361 caacaaaaag cttccgaacg tttaaatgca tactattctg gttctccact tcaatatagc
    15421 aaggatgaaa gagcgtatac caaaggggca tacatagtgg atataaaagc tggtgaaaaa
    15481 ccaataattg aagatgtata ttttaataat tacaaaccta tagaagtatt taaatgtaat
    15541 ggaatagaag aagcactaga tatatgtgag caaaatcaag atagagagat ttggtcatat
    15601 tttgaaataa atactgatga gataatatca cagaatgaaa ttaaaaaaat gaaggaacta
    15661 ttaaaagaca taatagaaat aaaaccaata ataacatctt gttatgaaca agaaagtgta
    15721 gatataaaag aaaagtctat ggcagagttg tttagagagt tctattcttt tagtaaaggt
    15781 gtagagccaa aaggagaact tatggattta tttctagata taataagtga agaaggtgaa
    15841 tcagcagatg agaccaatta gattagaact tacagggctt aatagctata ttgataaaca
    15901 agttatagac tttgaaaaat taattgaaag aggtttgttt gggatatttg gaactactgg
    15961 aagtggaaaa tctacaatct tagatgcaat aactattgct atgtatggaa atatatccag
    16021 aaatacaaaa gaatatataa acagtgtgtg cgataaggca attatatcat acgagtttga
    16081 gataggaagc aaaaacacta gaagaagata cattgtagat agaaccattg ctagaagcaa
    16141 aacaggaaca aagacctcta atgctagatt aatagaagta ttgaatgaca atacccaaaa
    16201 tgttttagct gataaagttg tggaagtaaa tgaaaaagta gcacaggttg taggattaac
    16261 ggcaaatgat tttactaggt cagtagtttt accacaagac aaatttaatg agtttctaag
    16321 attatcacgt gcagatagaa gagacatgtt agagagaatt tttaatcttg agaaatatgg
    16381 aagaagtctt ggagaaaaag tgaaaaagag aaaaaatata cagttacaaa atttaaaaga
    16441 tttgaaatca aaattaagtc aatatgatgg aattacagag gaagtataca acaaaataaa
    16501 ccaagaatta atagagttaa aaaatttgga aaaagataag aataatgctt tagacctagc
    16561 tcaaaaatct tatgaagaaa gtaaaacagt atatgaagaa caattaaagt tagaaaaaaa
    16621 tgaacttaga aaaaatgagc ttgatttaaa aaatagtgag ataaaagaaa agattgctct
    16681 tgtagaaaat gatagtaacg ctaaaaaggt agacccatac ataagttcag ttcaaaatct
    16741 agaaaaaaag ctagatgaag atagttttgc agtaagtaat ttagaaaaga aattagcaat
    16801 attaaatcag gagcttgaag ttacaaaaaa tagatatgaa aaaataaaca aaattaagaa
    16861 tgaagaagtc cctaaattga gtgaagaaaa aataagattg caacaagcaa taaaattaga
    16921 agaagagtta gttttattag atagagagtt aaaagagcta aaagaaagtg gaattaattt
    16981 aaataagact aaagttgaat tagaaaaagt aaagcaggtg tcagagtcca gaaaagatgc
    17041 agttactaaa tctataaaag aagttgaagg aaagattgat aaagtaaata taagtgcaga
    17101 attaaaacaa aaaatatttc tagcatatga atacgagaaa gattacaaca acgtattgga
    17161 agaaaaaaat caaaagctaa ataaactaga agaaatactc aaagagacag aaaatataaa
    17221 tttaaaagtt agatatattg ataaagataa gaatgatgta aatcgtaatt tagaaaattt
    17281 atcacttcac ttggatgttc ttcttaaaaa atgtcctggt aagagtgctg atttactttt
    17341 aaaatctgaa tatgttactg aattaagaaa taaagcaaat aatactaaag aaaatgaaat
    17401 taagaaaagc agtatacaag atgaattaaa gataatactt gaaagtaagt ttaatactga
    17461 aagggaagta aatttactaa atgaaaaact tgaaaataat agaaagaaca gagatgactt
    17521 agaaaaagaa ttggaagaac taaagtattt aaatttagcc tctgaactta gacgtgaatt
    17581 aaaagaaaat atgccttgtc cagtttgtgg gtcaaaacat catgaaaatc atgatataac
    17641 taagtatgat gaaaatatat cttttgtcaa agaaaaactg gagaaattag aaaaagaaaa
    17701 aataagtata agaaacaata tagaggaatt aaatgctaaa gtaagtgggt atttatctat
    17761 agaaaaaatg aagactaaag aacttgaaga tgtaaaagga aaactagggg ggattccatc
    17821 tagtcaatta ttaaaaaaac ttgatgaaga acaaagaaaa cttgcattgt taaaatctaa
    17881 tattcaggag tgggagaaag aaaaagagag tactgaaaat aaagtaacat tagctaaaga
    17941 agaaaaaaat aaaattgaaa aagaagaatt aaaaataaga gagagtctga ataattataa
    18001 gaaattaaca agagatttaa acatagaaat agaaggttta gaaaataaat gtaaaaaaat
    18061 taaacaggaa tacctaggat taaagacgat tactaaagtc tctaatctat tgtcaaaagt
    18121 aaaagaaata agagagaatg aaaaagaatt agaattacta agtactgggc attcaaattt
    18181 attaaaaaat agagactcac tagatattaa aataagagaa aatgagagta aactacatga
    18241 aatagagata gaattaataa aggctagaga attatatgta gaaaagaaat tgagtaggga
    18301 taataagtat aaagaagtta ttagcataac gaaaggtgat ttagctaaaa atcttttaca
    18361 taatgttgaa gaaaatatat gtaagatatt agaacaagaa gaatcaagta agaaaaaatt
    18421 ggaagagcaa agattagaat atgaaaaaaa cattgctgaa aagcacaata tagatggtag
    18481 acttaaaact gctaaggaac aatataaaga ccaaaaatca acactaaata aactattagc
    18541 agaaaataaa tttgaaagta tatatgcagt aaaaagagct ttattagata ctgatgcagt
    18601 aaaaaaattg attgaagaaa tactagagca tgaagaagaa caaaagttat tatcatttaa
    18661 aataaaatca tcaaaagaaa aattaaatgg aagaagcata aagaaggagt actttgaaca
    18721 gttaaaagat gaaatttata atttgaaagt agaaatagga aatatatcca aagatattgg
    18781 agctaatcaa aatcagttaa taacattaaa agactcttta gataagataa atgattttaa
    18841 taaacaactt aaggtagttg aacacaatgt ggacttattg gaagaattag ataaatgtgt
    18901 acaaggaaat aaatttgtag aatatgtttc tacaaaccaa ttaaaatata tagcattgga
    18961 agcatcaaaa cgattggatg gaataacaaa aggaagatat gcacttgaaa ttgactcaac
    19021 actaaacttt gtaatgagag ataactttaa tggaggagaa agaagaagca ttgatactct
    19081 atctggtgga gagacattct tgacatctct atcattagca cttgctcttt catctcaaat
    19141 acaattaaag ggaagtgcac cattagaatt tttcttttta gatgaaggtt ttggttcatt
    19201 agatagtgaa cttttagaaa ttgttataga atccttggaa agattgcata gtaataatct
    19261 aagtgtgggt ataataagtc atgtcgagga acttaaaaat agagttcctg ttaagctatt
    19321 agtttcatct agtgaggctg gaataggttc aaaagtaaaa attgaatata gttagaaaat
    19381 atatgttaat tgaatatatt atctaatatt tccaaataat atgtaattgt gatataataa
    19441 aaactaatat taaagttatt tataattaat attaatataa aaacatgtaa gtaggagtgt
    19501 ggtaagtgtt ggaatcgccc aataatttga ttcagattat ttttttaata gtattactta
    19561 tagggtctgc atttttctca gcatctgaaa cggccttaat gtcgttgagt aaaatcagaa
    19621 tcagatatat gcaagatgaa ggagttaaag gagctaagtt agtaagttca ttaatagaaa
    19681 atccaaataa gcttttaagt tctatattgg ttggaaataa cgtcgtaaat atagcagcaa
    19741 catccatatc aacatctttg tttataggat taatgggaga aaaaggagtg gcacttgcta
    19801 cagctgttat gacagtatta gtattaatat ttggtgagat tactccaaaa acaatagcag
    19861 caaataattc agaaaaggtt tctctattag tatctaaacc tataaaagca attatattta
    19921 tattaagacc aatagtatgg atatttaata taattacaaa tattatattt aaattatttg
    19981 gtataacaaa taaaggagct aaatcattca taactgaaga agagctaaaa actatggtaa
    20041 atgtaagtca tgaagaaggc gtccttgaaa tggaagagag agaaatcata aataatgttt
    20101 ttgaatttgg agatatgcaa gctaaaaatg caatggtaca aagaatagat atggtagcta
    20161 tagatatgga agatagttat gatgagataa tacaggtatt taaaactgaa aaattaagta
    20221 gaatgcctgt gtatgaagaa actatagatg atatcgtagg aatacttaat ataaaagata
    20281 ttatattttt atctgatgaa gaaatagagt catttgatat taaaaattat atgagagaac
    20341 cttttttcac gtatgagttc aaaaagataa cacaacttct tgaagagatg aaacttgaaa
    20401 aaagtcagat ggctatagtt gtagatgagt atggtggaac atctggactt ttaactatag
    20461 aagatttagt agaagtcatt gttggagata tagaggatga atatgatgaa gaagaggatg
    20521 aaatacaagt tataaaagaa gatgagtata ttgtagatgg aagtactaaa attggtgatg
    20581 ttaatgaact tattggtgta aacttggaat ctgaggagtt tgattccata ggaggtttta
    20641 taattggaca cctaagcaga ttgccagaag aaaatgaagt aattgaagtt gataatataa
    20701 gattttgtat agaaagtata gaaaaaaata gaattaaaaa aataagaata tatacataat
    20761 gtaataatgt tcttaaattt attagaagtg tataaatata aaagatatct tttatattta
    20821 tacacttctt tttattttgt attcaaataa aactagtatg aaatggacaa aataaaaata
    20881 taaattagaa gaaaggagat ttttaagatg agcatactag aaaataattt tttatacaaa
    20941 ttaaaaaaca acactatctt tttttgtata tatacattgt tatttatttt tatttacaaa
    21001 gcgtttccat acatagctcc attttttcta ggtgctataa tagcacttat gattaatcct
    21061 atatcacaaa agttagaaaa taagtttcgt ataaataaag gtatctctac tcttgttcta
    21121 agttttcttg cagtggctat agtcagtaca gttactacaa taatagtaat aaattcaatg
    21181 aaagaattaa tgggattttt aaataatata tctgctaatc ctgaggatat aagtaatact
    21241 attatgtatt ttttaaataa aataaatgac tttatgaaat catttcaaga aatagccaat
    21301 tttgatgtag aacaattagt aaacaaattc agtggtgaag taatgcaaat tacaaaaaat
    21361 cttcttacta gtatacttgg attggctact tctattccat atattataat atttataatt
    21421 accctgttta ttgctaccta ctttatagca aaagaccttg ataagattga aaatagtttt
    21481 tataatatgt ttactgttga tgttagaaag aaggtaaaaa acgtaaagaa agaggcaggg
    21541 ctttcacttg ttggatatat aagggcctat acaatactta tggctataac attttttgca
    21601 atctggggaa gttttgcttt atttggattg aaatacggtc taatagtagg gtttgtagga
    21661 gctttaatgg atttaattcc attcctaggt ataataacaa tatatcttcc agtaattgtt
    21721 tattatttcc ttataaaaaa ttattttata gctataagta tgactgtgat attttttgtt
    21781 ttgtctttga ttagagaaat acttgaacct aaactagtat cagtaaatgt tggactaaat
    21841 ccattagcta ccctagcagc gatttttatt ggaatccaag taaaaggaat aattggagta
    21901 attttttgtt tgggtttggt atgtatgcat gatatactta agaaggtgga tattttttaa
    21961 ttataataat tgatagattt ttttaaaaat aaaattttat tcacaggtaa ataaaaaaat
    22021 attaatctta ttaagaaaga gggtatatac gtgaaacaaa aagtagtgga aagattttta
    22081 aaatatgtat catttgatac aacatctaat tctcagtgtg agaactgtcc atcttcagaa
    22141 ggtcaaagag ttttagctaa gtatatagtt gaagagctaa aaacaatggg tgttgatgat
    22201 gtgagtttag atgaaaatag ttatataatg gctactttaa aaggaaatac tgatggtgtg
    22261 gatactatag gattcatatc acatttagat acaattgaag atgttagtgg aaaagatatt
    22321 aaaccaagaa taatagaaaa ttatgatgga aaagacatag ttttaaatga agcgttaaat
    22381 gttattacgt atgtaaagga ttctccagag ttggaagaat ttaaaggtga cgatttaata
    22441 gtcactgatg gaacaactct acttgggtct gatgataaag ctggtatagc tgagatagtg
    22501 actgcaatag agtatctaat aaatcatcca gaaattaagc atggagatat aaaaatagga
    22561 tttacaccag atgaagaaat tggtagaggt gctgatttat ttgatgttga aaaatttgga
    22621 gcaaaatatg catatacttt agatggagga atagttggag aacttgaatg tgaaaatttt
    22681 aatgctgcaa atgcaaccat tactatacat ggtagaaatg ttcatccagg ttcagctaaa
    22741 aataaaatgg taaatgctat acatatagct gctgaaatat cagaaatgtt cccagcagat
    22801 gaaagaccag aaacaacaga aggatatgaa ggattttggc atttaaattc tataggtgga
    22861 aatgtcgaga atgtaagtat ggcttatatt attagagacc attgtaaaga aaaatttgaa
    22921 aatagaaaat ctattatgat agagaatata gaaaaaataa ataagaaata cgataataga
    22981 gtggaattgg atttaaaaga ctcttattac aatatgaaag aaaagattga gccagttatg
    23041 tttatagttg atatagctaa agaagctatg gaggaattag gtataaaacc acgtttagtt
    23101 cctgtaagag gtggtactga tggagcaaga ttatctttta atggattacc ttgtccaaat
    23161 atatttactg gtggattaaa cttccatggg aaaaatgagt gtataccagt tagttctatg
    23221 gagaaagcta ctaaactaat tgtaagaata gctgaaaaat atgctgaaag agtttagtta
    23281 aaaagtattt aaaaattaag catttaaagt tataatagct atatcattct ttataagtca
    23341 agctgattat aagcttgtgt tgtacttaaa atggtatagc tatttttgta attattttta
    23401 taaaagataa ttatttttat aaaagataat tatttttagt ttagggtatc aataaaaagt
    23461 atgaatatca ataggtatta acatttaaaa gggggaattg tattgaaaat taaatctaaa
    23521 aaaataatag catttgcact ttcacttggg attatgttgc caagttgtgt atatgcagac
    23581 actattagta aaaatcaaaa tgcacaggta gatatgaaca attatcttca aggtagaaaa
    23641 tctccaaagg aggaaaaact tattggtgaa gatagatttg atacagcgat aaaaatatct
    23701 caaagtggtt ggaataatgg ttcagaaagg gtgtttctag ttaactcaaa ttcattgcca
    23761 gatgcattgg cttcaactcc tttggcaagt aaattggatg cgccaatact gcttacaaat
    23821 aaaaatagta taccatataa tgtaacagag gaaataaaaa ggcttaatcc agcagaagtt
    23881 attttaattg gaagtgaagg agctatttca tcaagtgtaa aagaatctct tgaagatatg
    23941 gaagtattag tcagtagaat cggtggagca gatagagaag aaacatcttt acttcttact
    24001 agacagctag atgatactgg tgatttaggt gtctcaaaag tggcagtggt taatgggtat
    24061 aatggactgg cagatgcaac ttcaatatca agcccagctg caagtgataa tacagctata
    24121 atatatacag gtaaggatag cataagaagt gaggcaaaga attttattac acaaaactca
    24181 actaagacat acatagtagg tggggagtac agtataagta aaaaacttga aggacagtta
    24241 gtaaactcag aaagactagc agggacagat agaaaagata ctaatgcgaa agttttagaa
    24301 aagttttatg gaaaatcaag taaggttaat aatatgtatt ttgcaaaaga tggaagtggt
    24361 agagaagctg atttagttga tgggcttgct gctggagtat tagcatcaaa aacaaagtca
    24421 cctgtagtac ttgcaagtgg aagtttaagt agtgcacaga aaagttttat aaaaaaagta
    24481 aaggctgata aatttgtaca agttggtgga ggtaaaaata gtaaaccata tgcagaagca
    24541 ttagccttac aataatattg tgtaaatcaa aattaatata taataatagc aagtaaacca
    24601 agttgcactt caatatatat aataaaataa gtaaactaag ttatatttca gtataatata
    24661 taatgaaata agtaaactaa gttatactcc aatataatat ataatagaag caagtaaact
    24721 aagttatatt tcaatatagc atataataaa acaagtaaac cagattctat ttaatt
//
LOCUS       DAELLV010000032        23591 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.032, whole genome shotgun sequence.
ACCESSION   DAELLV010000032 DAELLV010000000
VERSION     DAELLV010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23591
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.032"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..365)
                     /locus_tag="KPD58_001102"
     CDS             complement(<1..365)
                     /locus_tag="KPD58_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264885.1"
                     /translation="MFGYVKINKMDLTFREYDYYKAYYCGLCKYLKRNHGEISRFSLN
                     YDITFLIVLLTAVYNPESISTEEVCIVNPFKKKKVITNDITEYAASMNILLTYYKLED
                     NLMDDKRIKDKLAYYIYKN"
     gene            complement(584..1969)
                     /locus_tag="KPD58_001103"
     CDS             complement(584..1969)
                     /locus_tag="KPD58_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0264886.1"
                     /translation="MDNLNKKFLMEYIDEIQGELRDISDKIWKNPELQYKEYYASNLQ
                     KECLKKHGFSIEGLEGMDTAFVASYGEGRPVIAVLGEYDALDGLSQKVSTTKEPIVDG
                     GAGHGCGHNLLGTGSIGAVIAVKELIKLKKLKGTIKYYGCPAEEDLSGKVLMIKKGFF
                     DGIDCAFSWHPFDINTPIRIPTLANYSVKFRYNGISAHAAQAPYNGRSALDAVELMNI
                     GCNYLREHIFDNCRVHYVTTNGGKMPNIVPDFAEVWYYIRGVKMEHVRDVFGRIVDIA
                     KGAALMTGTIMEYDIISGVYDYIPNTILTDILSQNMKLVGVQNYNEDDYYFADKLAET
                     VSIDKRSSVSSVLSGNKDITKMNLHDEVTDDTFTHNNCISLSLDIGDVSYIIPTAQCS
                     CSVWPIGISAHTWQSCASAGSDMGFKAMLLASKSISCSINDVMLDELVINKAKKELKD
                     TVGSFKYIPII"
     gene            complement(2142..2885)
                     /locus_tag="KPD58_001104"
     CDS             complement(2142..2885)
                     /locus_tag="KPD58_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HBG0264887.1"
                     /translation="MKKVLIVGESWVKNITHIKGFDTFVTTHYEEAVKWLKEAIESGG
                     YETVHMPAHVAADSFPYKLEELNEYDCIILSDIGSNTFLLSNSTFIDCNSNPDRLELI
                     KEYVNNGGALIMVGGYMSFTGIDAKARFGETAIKDVLPITMIDKDDRVEKPAGIIPEV
                     IDSEHPVLKGIPTEWPKFLGYNKTVARDNCPVLATIGGDPFVAVGEFGKGKSAIFSSD
                     CAPHWGPKEFTDWKYYNKLWVNMLDWLTC"
     gene            complement(2905..4167)
                     /locus_tag="KPD58_001105"
     CDS             complement(2905..4167)
                     /locus_tag="KPD58_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG0264888.1"
                     /translation="MKKILLDTLNSKKQEYIDYLKELVSIKTEDVGHGILGGFEKEGQ
                     EYIEKLANYIGFSVDRQEMSEELIKKAKNIYKEGNLGHNYQDRYNLICKYSDDLPGKT
                     IVFNGHVDTMPPGDISKWKYNPYRATEDNGKLYGLGTADMKSGLIASILAVKLIKDSG
                     LNVPGNVKIMSVVDEEGGGNGTINAVMNGIDGDCCIICEPSEQNLIVAHMGFVFFEVE
                     VKGVSLHCGSKWEGVNAIEKAMLLLQDIKELEHNWLMIYKHPLLPSPTINLGVINGGT
                     AGSTVPDKCVFNLCVHFLPNIMSYEQVVNDVTNVIMTRANGDLWLKDNKPNINIYQSG
                     LGFEMDKDSDFVVNAHKILEETLGKKLEIKGSTAGNDARVMKNLAEIPTLILGPGSIE
                     QCHSIDEYVEIKEYLDSILMYASLILNL"
     gene            complement(4351..5349)
                     /locus_tag="KPD58_001106"
     CDS             complement(4351..5349)
                     /locus_tag="KPD58_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HBG0264889.1"
                     /translation="MSSITKHKEEKSSPLLTKLTSVFRDQGSAAVGLVIIFIIMSVAS
                     SNFLTLDNLINVGRQISINAILAVGMTFVIITGGIDLSVGAVIALVGTFWATTVVNYN
                     APIWVGMILALAIGTILGVIKGAIISTQKLPPFIVTLAMLTIISGASFVFTGGRPISV
                     NTDAFKMLGRGYIGPIPIPVIIMIIVVIAGHFLLKRTNFGRHVHAVGGNEEAARLCGV
                     KVNKVIVKVYALAGLLTALAGIILSSRLASGSPTVGDGAELDAIAAVVLGGTNMMGGS
                     GSIVGTCIGVGIIGILGNGLNLLSVSSYNQMIIKGLVMLFAIWINNIKLKKSAKSK"
     gene            complement(5333..6844)
                     /locus_tag="KPD58_001107"
     CDS             complement(5333..6844)
                     /locus_tag="KPD58_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBG0264890.1"
                     /translation="MSNIILKLSNIAKEFPGVRALDNVNFELFHGEVHALLGENGAGK
                     STMIKILTGAHSKTSGKFIFEGKEIENISPDISKKIGINAIYQELTVFDELTVAQNIF
                     MGKEINGKVLTNDKKMNEEAKKIFDNMGIDINPNSLVKELSIAQKQMVEIARVLSSET
                     KVLIMDEPTSSISKKETEILFRLINDLKESGVSIIYISHRMEELFEICDRITIMRDGK
                     TISTLNTKDVSSEEELVNLMIDRKLDQFFPKRKVEIKEEIMRVENLTKNNVFNDISFN
                     IRKGEILGIGGLVGSKRSEIVEAIFGLRTYDSGKIYLNNEEVKFKTPSDAIENGLGLI
                     TEDRKGTGLFLQMSVKENTTMAGLKKISKFKSIIDRKKEKEILEKYIEALKIKTPHMN
                     QVIQSLSGGNQQKAIIARWLLLQPDILIMDEPTRGIDVNAKAEIYNLMGDLVESGVSI
                     IMISSEIPELISMSDRIMVMREGHISGFLEGEEMVENNVLKLAFGGKINEFNN"
     gene            complement(6898..7860)
                     /locus_tag="KPD58_001108"
     CDS             complement(6898..7860)
                     /locus_tag="KPD58_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBG0264891.1"
                     /translation="MRVRKILSLGIAGILAIGMLTGCSMEGPSKSDNKGGSDKKDLTI
                     GVSTITLQHQFFIDIDEGIKEKAKELGVKVIVNDPDQDVAKQTSAIEDFIQQNVDGMI
                     VLGTDNSAIVPAVEGAFEKMPVVTVDAVLNTENITSYVGTVSYDAGKKLGEYTKKYID
                     EKLGGKSEIAIVTDLKSQIQMQRIDGFKDALKGSANVKILNSQPGYDREESLNTVENL
                     IQSNPDVDIIYATAENSVLGAKAALESAKNKDVKIVGFDLTEEASSGITDGTILAMIQ
                     QQPKEMGRLAVEAVVKAIKGEKVEKNIPVPALLYDKENIKDFKN"
     gene            complement(7908..8798)
                     /gene="rbsK"
                     /locus_tag="KPD58_001109"
     CDS             complement(7908..8798)
                     /gene="rbsK"
                     /locus_tag="KPD58_001109"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBG0264892.1"
                     /translation="MGNIVVIGSVNMDMVCSVDKRPEKGETVLGNSFFTSPGGKGANQ
                     AISASKLGANVKMISCIGEDGLGEELIRNFRTNKVDYSLVSRNNHKSSGVAVITLCEN
                     DNSIVVVPGTNELVDIELIKKNEEEIKNADIVLLQLEIPLKTINYVVNFCFENRIRVL
                     LNPAPAVKLNEDIIEKVTYLTPNEHEYKIVFDTNEGIEEVLKKYPNKLVITEGKNGAR
                     FYDGEEIKHVSCISVDVQDTTGAGDTFNGALAVAITEGKNLYTAVEYAVVVSGLSVTK
                     LGAQSGMPYKEDVEKYLNNK"
     gene            complement(8840..9853)
                     /locus_tag="KPD58_001110"
     CDS             complement(8840..9853)
                     /locus_tag="KPD58_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG0264893.1"
                     /translation="MSITIKEIGELAGVSKTTVSKVINNKDENISQATREKILKIMKE
                     KNYVPNKLAQSLVTKKTNTIGLLIPDIRNPFFTDVSRGVEDKANEEGYNIILCNTDED
                     AKKEYEGIRTLSERMIDGIIFAASSNTNWKEANYKDIKIPTVLIDKKISMNKETLKGI
                     VKINNFEGAYIATKHLLDIGNKKIIYLSGPLQNEIAVDRLEGYKKALIECNLSYNPAY
                     VFEGKYKIEWGQEFIKNLEKIDFDAIFCANDLIAIGVIRGLKERGLSIPNDISVVGFD
                     DIQTSSLISPSLTTVKQPSYDIGYKASEILINCLRGDKKESFDELIFKPELVIRDSTK
                     ESE"
     gene            complement(10082..11059)
                     /gene="bioB"
                     /locus_tag="KPD58_001111"
     CDS             complement(10082..11059)
                     /gene="bioB"
                     /locus_tag="KPD58_001111"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HBG0264894.1"
                     /translation="MEKYIIKLKNKVLREKEISYEEALNLISLDTNNKNDFDILLKSA
                     NEIREYFMGRKADLCTIMNAKSGKCSEDCKFCAQSSYYKTGVEEYSLLDYNEILNRAK
                     EMESKGVHRFSLVTSGKGMSGKEFNNILNIYEGLRENTNLKLCASLGIIDYEQAKMLK
                     SAGVTTYHHNVETCRDNFHNICTTHTYKDRIKTIKDAKKAGLDVCVGGIIGMNESEEQ
                     RLKMAFEIRELNVKSFPINILNPIKNTPMENYDVLEPMEILKTTAVFRFIIPNVYIRY
                     AGGRLSLKGYDKVGFNGGVNSAIVGDYLTTVGSGIENDKKMIIEQGFEF"
     gene            complement(11265..11528)
                     /locus_tag="KPD58_001112"
     CDS             complement(11265..11528)
                     /locus_tag="KPD58_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264895.1"
                     /translation="MKIYYEKENSKKANVNNIPSVFAFNFLRNFLTSKEYEETRKEYF
                     INNLTKSQVNQAMKDLKWLFREYEGLEVITMESLDGVKTRIVL"
     gene            complement(11598..12671)
                     /locus_tag="KPD58_001113"
     CDS             complement(11598..12671)
                     /locus_tag="KPD58_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HBG0264896.1"
                     /translation="MEKSKVYFCDLYSNSQNKNVPNNVRRLFDEAGFKDLIEKNDQVA
                     IKLHFGEKGNTTYMSPVAVRQVVDKVKDCEGKPFLTDTNTLYTGSRTNSVDHLTTAIE
                     NGFAYAVVNAPVIIADGLYSRNYENVKIDKKHFESVKIGGEIYNSSAMIVMSHFKGHE
                     AAGFGGALKNLAMGCASAAGKQMQHSDVTPVVKEKKCVGCGKCVNSCPTKAISIVDKK
                     AVIDSDVCYGCGECPTVCPTRAVTIQWESDSDVFVEKMAEYAYGAVSNKKDKVGYITF
                     VMNVTPLCDCVPWSGRPIAHDIGILASTDPVAIEQACYDLICKEMGHDVFKHEHPHVN
                     GTRIIDYACEMGMGSKEYELIKL"
     gene            complement(12930..13688)
                     /locus_tag="KPD58_001114"
     CDS             complement(12930..13688)
                     /locus_tag="KPD58_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0264897.1"
                     /translation="MREYIAFTKKEFKENLRNYKLFSLIILFLIFGISSPISAKFMPD
                     LIAHFAPTLKVTAAPTALDSWTQFFSNISGLGMSLTLIIFCNILSNEYSKGTLVIMLT
                     KGLSRSSIVLSKFSVTVIIMTIGFWLSFLFAYGYTMYFWPTANLNHIIFSAFNLWLIG
                     IMYISVLILGCVLFRPAFASVLLVLVATLLLSLISIPKQIAPYTPNFLMSKNIDLISG
                     KVAMPEFIIPIIITVLISIVCLLAAVILFNKKPV"
     gene            complement(13685..14602)
                     /locus_tag="KPD58_001115"
     CDS             complement(13685..14602)
                     /locus_tag="KPD58_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0264898.1"
                     /translation="MNIVSINGLSKGFGNRKIIDNLNFTVPEGSVFGFVGKNGAGKTT
                     TMKMVLGLLKPDGGTIDVCGEKVTYGKTSSNRHVGYLPDVPEFYNYMRPLEYLSLCGE
                     ITGLSKKEIQIRSEELLSLVGLGNEKRRIGGFSRGMKQRLGIAQALLSRPKLLICDEP
                     TSALDPVGRKEILDIMLKIKDSTTVIFSTHILSDVERICDHVAILNKGSIALSGTLSE
                     IKSMHGKDRLLLEFASNDEIQKFKSSDGIKSLLKDSEETNMEIVLHGKDIKAIQKTVI
                     STLAEMNLCPVKMELIELSLENLFLEVVK"
     gene            complement(14602..14802)
                     /locus_tag="KPD58_001116"
     CDS             complement(14602..14802)
                     /locus_tag="KPD58_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc_N domain-containing protein"
                     /protein_id="HBG0264899.1"
                     /translation="MENLMEYLPLLVPVIILDLILIITALVHVLRHPNYKIGNKAIWI
                     IVVLFISLIGPILYFTIGRGEE"
     gene            complement(14807..15901)
                     /locus_tag="KPD58_001117"
     CDS             complement(14807..15901)
                     /locus_tag="KPD58_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0264900.1"
                     /translation="MNELNIAKTLILKRKEKGITQDELANYIGVSKASVSKWETGQSY
                     PDITFLPQLATYFNITVDELICYEPQMMKEDINKLYNKLCKDFTVKPFDEVMDEIREI
                     IKKYYSCFPLIFRMGLLIVNHYDIVDEKKRELLISEALEIFIRIQETCNDIDICRQAK
                     SMEATCYILLNQPIQVIDLLQNSNFPMINESILLAQGQMMNGQMDEARETFQLGAYQN
                     LISLVQNLVGILQNADKLQMKEIERRILAISDIFELDTLSPAIMLSSYLTQAQINLIH
                     GDNEGAIKSLRKYVDLATRDIYPIIIHGDDFFNKLDRWISEIGTGITRDDTIVKAGIV
                     AAIKNNPMFSVLSENKEYKFLIEKLSLLEE"
     gene            complement(16411..16551)
                     /locus_tag="KPD58_001118"
     CDS             complement(16411..16551)
                     /locus_tag="KPD58_001118"
                     /inference="COORDINATES: protein motif:HMM:NF021468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HBG0264901.1"
                     /translation="MNGEGCILSNKITKECYKVGYMIREKSIDIYPDSGWGFMTWDEN
                     GE"
     gene            complement(16861..18168)
                     /locus_tag="KPD58_001119"
     CDS             complement(16861..18168)
                     /locus_tag="KPD58_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG0264902.1"
                     /translation="MDISRSVDNSLNRTIEFLKELIKIDSQQGEPISQCPFGIGPKKS
                     LDKTLDYCASLGFSVKNIDNYIGYAEIGEGEELIGIPMHLDIVPPGEGWSVDPFSGAV
                     IDNIIYGRGVIDNKGAVSMLIHVLKNIEDMYPTINKRIRLIFGTNEETGMKCIKYYLD
                     KGEEIPSMGFTPDAMYPVVNGEKGRVHIRIEKEIKIDKSKPYIIVSGGTKENVVPSHC
                     TAKIINGIISELTTKGVAVHASNPEKGENAISKMVIKIVEDNMDFQHREDIELVSKYL
                     CSDYYGDALGINQYDEVFKNTTLNLGILKVNEEKIVCELDIRYGKNIVLNNIIDRFKK
                     VFCNGWKIEVIAHKDLHYVDESNLVLKKLLEAYEEVTDENGYTIAMGGGTYASWFKDM
                     VAFGPKFLAYKTGGHGVDERVPINHIRKNMEIYTLALIKLLEL"
     gene            complement(18233..18712)
                     /locus_tag="KPD58_001120"
     CDS             complement(18233..18712)
                     /locus_tag="KPD58_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HBG0264903.1"
                     /translation="MSEKMYTEKEYLEGIRGATGERAVWFYLLMKEAEKLGANPDDIC
                     KEAIYGFGKMRGQKYNVADTPGKMAEMLYNSKGQKVFEMELVENTDENGVLKFHHCPL
                     DAAWKEYGLTKEERKEICRLACYGDYGRVDCAQGVKLDFAQKCAHDDEVCELVFTKK"
     gene            complement(18759..19622)
                     /locus_tag="KPD58_001121"
     CDS             complement(18759..19622)
                     /locus_tag="KPD58_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG0264904.1"
                     /translation="MQEIKNKEVKNGQGPTEVNKTRVLTKKDVTKTYLRWWWTAELSN
                     SFERMQALAVCASFTPALEKLYKRKEDLVDALKRHLQFFNTQAIWGGLIHGTVLAMEE
                     EKATEGKIPGEVISGVKNGLMGPLAGIGDTLDFGTFQTIFLALGASFGAEGSVIGAFF
                     PIMFSILLFCEGYYLFHLGYSLGRDSIKKILSGGIVNKIIDGASILGMFMMGALSATT
                     VKLSTPLSFDIGGKAIVVQDTLNMIAPGLLPLGVVFFVYWGMKYKKWTITKLLVILVV
                     LALVGSFIGIF"
     gene            complement(19622..20377)
                     /locus_tag="KPD58_001122"
     CDS             complement(19622..20377)
                     /locus_tag="KPD58_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0264905.1"
                     /translation="MESVLMLAIVTGLWYWFAAGLAGYTLFSTLKSPLFIGFSLGLLW
                     GDVTTGMIVGASIEMVYLGMVAAGGNIPSDKCLAALIAIPVALQTGVNAEVAVSIAVP
                     LGVIGVLVNNLRRTGNAVLVHKADKYAEEGNTKGIWRCATLYSLIFGFVLRFPIVFVC
                     NFFGADLVQSLLDVIPQWLMNGLTVMGGILPALGFATTIFTIGKNKFLPMFIIGFFMV
                     QYFEISITAAAIFGVCIALLITFMKEDKRVGEV"
     gene            complement(20404..20892)
                     /locus_tag="KPD58_001123"
     CDS             complement(20404..20892)
                     /locus_tag="KPD58_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0264906.1"
                     /translation="MAKITLVRVDHRLIHGQVITKWVKIAQAQKIIIVDDFLGQDEFM
                     ADIYKMAAPSGVEVVILTAEDAGQAFQNNTLGDKNIFILFKNVDMANKAYKAGLKYEK
                     IQLGGIPNEAGKKMVFTAVSLGNEDVEQLNELNENGVEIVLQVIPEESSMTYENALKK
                     FK"
     gene            complement(20910..21311)
                     /locus_tag="KPD58_001124"
     CDS             complement(20910..21311)
                     /locus_tag="KPD58_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HBG0264907.1"
                     /translation="MNKYKVIILCHGTWGKVLVEETNKNFGLSNQYEVLSLSSEKDVS
                     VFMKEVEEVVDKEDVKIIISDLYGGSTSSVAIAVAIRRGIDAINGLSLQTILIVDEEL
                     SKEDKLHTLPERIVKKNNNLCVDLIKEFKKI"
     gene            complement(21314..21811)
                     /locus_tag="KPD58_001125"
     CDS             complement(21314..21811)
                     /locus_tag="KPD58_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0264908.1"
                     /translation="MSLVKYCRCDDRLIHGQVIYKWVKHLGVKKIVVVDDETTNDVIA
                     KGLIKMAAPKNIDLSILTVSESRRYFYNNQADDNVFVLIKNLDTANRMIEEGINIKKL
                     IVGRIPTGIGKKKISQNVYINKKEFLLIDEFIKKNINVSIQMVPDEEEVELNQNINRY
                     KGEFI"
     gene            complement(21835..22239)
                     /locus_tag="KPD58_001126"
     CDS             complement(21835..22239)
                     /locus_tag="KPD58_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0264909.1"
                     /translation="MIGIIVMTHGSFSEEIIKSCELIAGPAERTAAIKLNRNDNIEDL
                     NKNFVEKLNELDEGDGVLVLADLLGGSPSNVASLNLKKGGKFHALTGVNLPMLLEALI
                     NREGKSLEELAEACIEAGQTGINNINKVLSSM"
     gene            complement(22469..>23591)
                     /locus_tag="KPD58_001127"
     CDS             complement(22469..>23591)
                     /locus_tag="KPD58_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG0264910.1"
                     /translation="AYNNIVNDSIATTVKYILQKHDKWSKREGLDKLIKAIALHIQNL
                     IERIKIGNIVKHPNKDEIMKERAYEYNIAKEILSNMSLMYGVEFPEDEAIFLATFLYL
                     SYVGLNEESIAILVIMHGESTASSMVNVANTLLDCNHAVAINMGLEDRVQDILVQAVE
                     IANKIDKGKGILILTDMGSILTFAKVIRDATGKEVKAIDMVSTPIVIEATRKALTPEM
                     TLEKLYFNIIESIKKHYGDMEVTYAEKDNGGRYFDRLLIDNISKTLTFLNGEKAYFIL
                     KEVINRISEHYSLVIQDELLVKFIFHCSCMIERVIIKESLVYKSYEERISRNKELYLV
                     IKESFKLVEETFDIIISDMEYANILDIFESQYDTDKFKS"
BASE COUNT         7333 a         4496 c         2437 g         9325 t
ORIGIN      
        1 ttgtttttat atatataata tgccaattta tcttttatgc gtttatcatc cattaaatta
       61 tcttccaatt tataatacgt taaaagaata ttcatacttg ctgcatattc tgtaatatca
      121 tttgtaatta cctttttctt tttaaatgga tttactatac acacttcctc tgtacttata
      181 gactctgggt tatatactgc tgttaataaa acaattaaaa atgttatatc atagttcaaa
      241 gaaaacctag agatttctcc atgatttctt tttaagtatt tacatagtcc acaatagtaa
      301 gctttgtaat aatcatattc tctaaatgtt aaatccattt tgtttatttt aacatatcca
      361 aacataaata ctcctttttg tactattttt tatttagatt atatatagta taacactttt
      421 tatcaagatt atataattta cagtattcta agaaacttaa aaaattcctt ctaatcattg
      481 tatcttaaat ttaaaataat tagaaggttg ggttaaaaat aatattaaat tatatactta
      541 tattaactca tacaagttag ttatgcttat atcaatttaa gttttaaata attggtatat
      601 atttaaaact acctacagta tccttcaatt ctttttttgc tttgtttata accaattcat
      661 caagcattac atcatttatt gaacatgata tagatttaga tgccaataac attgctttaa
      721 atcccatatc agaacctgca ctggcacaag attgccatgt atgggcagaa ataccaatag
      781 gccaaactga acaagaacac tgtgctgtag gaattatgta gctaacatct ccaatatcta
      841 atgataatga tatacagtta ttatgagtaa atgtatcatc cgtaacttca tcatgcagat
      901 tcatcttagt aatatcctta tttccactta atacactaga aacactactt cttttatcta
      961 tagacactgt ctcagctaat ttatctgcaa aatagtagtc atcttcatta tagttttgca
     1021 cacctactaa tttcatattt tgtgatagaa tatctgttaa tattgtattt ggaatatagt
     1081 catatactcc acttataatg tcatattcca taatagtacc agtcattaat gctgctcctt
     1141 ttgctatatc aacaatccta ccaaatacat ctcttacatg ctccatttta actcctctta
     1201 tgtaatacca cacttctgca aaatcaggaa ctatatttgg catttttcca ccattagtag
     1261 ttacatagtg tactctacaa ttatcaaata tatgttctct caaataatta caacctatat
     1321 tcataagttc tactgcatca agtgcacttc ttccgttata aggagcttgt gctgcatggg
     1381 cactaatccc attatatcta aatttcacag agtaattagc aagtgttggt attcttattg
     1441 gagtatttat atcaaatgga tgccaactaa aagcacaatc aattccatca aaaaaacctt
     1501 tttttatcat caatactttt ccagataaat cttcttctgc tggacatcca tagtatttta
     1561 tagtcccttt cagctttttt agtttgatta attcctttac tgcaattaca gctcctattg
     1621 aacctgtacc aagtaagtta tgtccacatc catgacctgc accaccatct acaataggtt
     1681 ctttagtagt ggatactttt tgagacaaac catctaaagc atcatattct cccaaaactg
     1741 ctataactgg tctaccttct ccataactag ccacaaatgc tgtatccata ccttctaatc
     1801 cctctatact aaaaccatgt ttttttaaac attctttttg taaatttgaa gcatagtatt
     1861 ctttatactg aagttctgga tttttccata tcttatctga tatatctctt aattctccct
     1921 gtatctcatc tatatattcc atcaaaaact ttttatttaa attatccata ttaattatat
     1981 aaacctccct gtttctaata acttatcgat tatcagccaa aatgactaat tttaactgat
     2041 ttaaaaatat tttctaccat acaaccattt tatacaataa aacctcttga cttgtaagtt
     2101 tcattttatc aagtaagagg ttttcaagtt ttataaaatt tttagcatgt taaccaatca
     2161 agcatattaa cccataattt attatagtat ttccaatcag taaattcttt tggaccccag
     2221 tgaggtgcac agtcagaact aaagatagca gattttcctt ttccaaactc gccaacagct
     2281 acaaatgggt ctcctccaat agttgctaaa acaggacaat tatctctagc tactgttttg
     2341 ttatatccta aaaactttgg ccattctgtt ggtatacctt ttaatactgg atgttcagag
     2401 tcaataactt ctggaattat tcctgctggt ttttcaactc tatcatcttt atctatcatt
     2461 gttattggta atacatcttt gatagcagtt tctccaaatc tagcttttgc atctattcct
     2521 gtaaatgaca tatatccacc taccatgatt aacgccccac cattatttac atactctttg
     2581 attagttcaa gtctatctgg attactattg caatctataa aagtactatt agataataag
     2641 aacgtattag aacctatatc agataatatt atacagtcat actcatttaa ctcttctaat
     2701 ttatacggaa aactatctgc tgctacatgt gcaggcatat gaactgtctc atatcctcca
     2761 gattctattg cttctttcaa ccactttaca gcttcttcat aatgagttgt aacaaacgta
     2821 tcaaaccctt ttatatgagt tatatttttt acccaagatt cccctactat taatactttt
     2881 ttcatttata tattcctcct acttttataa gtttaatatt agacttgcat acataagtat
     2941 actatctaaa tactccttaa tttcaacata ttcatcaatt gaatgacatt gttctattga
     3001 acctggccct aatataagtg ttggtatttc agctaaattt ttcattactc tcgcatcatt
     3061 gccagctgtt gaacctttta tctctaattt ttttcctaga gtttcttcta gtattttatg
     3121 tgcattcact acaaaatcag aatccttgtc catctcaaaa cctaaccctg attgatatat
     3181 gtttatattt ggtttattat ctttaagcca taaatctcca ttagcccttg tcattatgac
     3241 atttgttaca tcatttacaa cttgttcata actcattata tttggcaaga agtgtacaca
     3301 taaattaaat acacacttat ctggcacagt agaacctgct gttccaccat ttattacacc
     3361 taaatttatt gtaggtgaag gtaatagtgg atgtttatat atcataagcc agttatgttc
     3421 caactctttt atatcttgaa gcaacaacat agccttctca attgcattta caccctccca
     3481 cttactacca cagtgaagag acacaccttt aacctcaact tcaaagaata caaatcccat
     3541 atgtgcaact attaagtttt gttctgatgg ttcacaaatt atgcaacaat ctccatcaat
     3601 accattcata actgcattta tggtaccatt tccgccacct tcttcatcta ctactgacat
     3661 tatttttaca ttaccaggta catttagacc actatctttt ataagcttta cagctaatat
     3721 tgatgctata agtccagatt tcatatcagc agttccaaga ccataaagtt ttccattatc
     3781 ttctgtagca cgatatggat tatatttcca cttacttata tcacccggtg gcatagtgtc
     3841 tacatgacca ttaaatacaa ttgtcttacc aggtaaatca tcactatact tacaaattaa
     3901 attatatcta tcttgataat tgtgacctag atttccttct ttatatatat tttttgcttt
     3961 tttaattaat tcttcgctca tttcttgtct atccacacta aaacctatgt aatttgcaag
     4021 tttttcaata tattcctgtc cttctttttc aaatccacct agtattccat gaccaacatc
     4081 ttcagtcttt atacttacaa gttcttttaa ataatctatg tattcttgtt ttttactatt
     4141 taatgtatct aagagaattt ttttcatatt tcctcctaag atttattttt acatttaaaa
     4201 tttcaagtct aatctcatct atttaagcca tagtaaactt tttcacttag catattctca
     4261 ttcaatgtat aaagatgtat gcaaattaaa gtataaaaat atatacaaaa acttaataat
     4321 ttttatagtt tataagctaa aatttaatta ctatttagat ttagctgatt tttttaattt
     4381 tatattattt atccatatag caaataacat taccaaacct tttataatca tttgattata
     4441 tgaagataca cttaataagt ttaatccatt tcctaatatt cctattattc cgacacctat
     4501 acatgtacct actatagaac cagaaccacc catcatatta gtaccaccaa gcacaacggc
     4561 tgctatagca tccaattctg ctccatctcc aacagttggt gaacctgatg ccaaacgtga
     4621 tgatagtatt atcccagcta atgctgttag tagtccagct agtgcgtata cttttactat
     4681 aactttattt acttttactc cacataatct agcggcctct tcatttccac ctactgcatg
     4741 tacatgacgt ccaaaattag ttctttttaa tagaaaatgt cctgctatta caactataat
     4801 cataattata actggtattg gtattggtcc aatatatcct ctaccgagca tcttaaatgc
     4861 atctgtattt actgatatag gtcttcctcc tgtaaataca aagcttgccc cacttattat
     4921 cgttaacatg gctaaagtta caatgaatgg aggtagtttt tgtgttgaaa taattgcacc
     4981 ttttatgact cctaatattg tccctattgc taatgcaagt atcataccta cccatatagg
     5041 tgcattataa ttcactactg ttgtagccca aaatgtacct actaaagcaa ttactgctcc
     5101 tacacttaaa tctattcctc ctgtaattat tacaaatgtc atacctactg caagtattgc
     5161 atttattgat atttgtcttc caacattaat taaattatct aaagttaaaa agttagatga
     5221 agctacactc attattataa aaattattac aagtcctaca gctgcactcc cttggtctct
     5281 aaatacactt gttaattttg tcaataatgg agaagatttt tcttctttat gcttagttat
     5341 tgaactcatt aattttacct ccgaaagcca attttaatac attattttca accatttctt
     5401 caccttctaa aaatccactt atatgacctt ctctcataac cattattctg tcactcattg
     5461 atattaattc tggtatttca gaagatatca ttattatact tacaccactc tccactaaat
     5521 ctcccattag attgtatatc tctgccttag catttacatc tatacctctg gttggttcat
     5581 ccataatcaa tatatctggt tgtaataaaa gccatcttgc tattatagct ttttgttggt
     5641 ttccaccact taaactttgt ataacttgat tcatatgtgg tgtttttatc tttaaagctt
     5701 ctatgtattt ttctaaaatc tctttttcct tttttctatc tataattgat ttaaacttag
     5761 atatcttttt taacccagcc atagttgtat tttctttgac actcatttgt aaaaacaatc
     5821 cagtaccttt tctatcttct gttattaatc ctagtccatt ttctattgca tctgatggag
     5881 ttttaaactt tacttcttca ttgtttaaat atatttttcc agaatcatat gttctaagtc
     5941 caaatatagc ttctactatt tcacttctct tacttccaac tagtccacct attcccaata
     6001 tttcaccttt tcttatatta aagctaatat cattaaaaac attgttctta gttagatttt
     6061 ccactcgcat aatttcttct tttatttcta cttttctttt tgggaaaaat tggtctagct
     6121 ttctatctat cattaaatta actaattctt cctcagacga tacatctttg gtatttaaag
     6181 tcgaaatcgt tttcccatct cgcattatag ttattctgtc acaaatctca aagagttctt
     6241 ccatcctatg ggatatatat attatactaa ctccactctc ttttaaatca tttataagtc
     6301 taaaaagtat ttcagtctct tttttactta ttgaagaagt aggttcatcc attattaata
     6361 ctttagtttc tgatgataaa actcttgcta tttctaccat ttgtttttga gctatactaa
     6421 gctctttgac taaactattt ggatttatat ctattcccat attatcgaaa atctttttag
     6481 cctcttcatt catttttttg tcattggtta agactttgcc atttatttct tttcccataa
     6541 aaatattttg tgctactgtt aattcgtcaa aaactgttag ttcttgatat atggcattta
     6601 tacctatctt tttagagata tctggggata tattctctat ttctttgcct tcaaaaataa
     6661 attttccaga agttttcgaa tgtgcaccag ttaaaatctt tatcattgtt gatttaccag
     6721 caccattttc acccagtagt gcatgaactt cgccatgaaa taactcaaaa ttaacattat
     6781 ctaatgctct gacaccagga aattctttag caatattact taacttcaga attatattac
     6841 tcataatgac actccttttt aaataggatt tctttcaaat aatctcattt tattttacta
     6901 attcttaaag tcttttatat tttccttatc ataaagtaaa gctggtactg gtatattctt
     6961 ttctaccttt tcccctttta tagcttttac tactgcttct acagctaaac gtcccatctc
     7021 tttaggttgt tgttgaatca tagcaagaat agttccatct gttattccac ttgatgcttc
     7081 ttcagttaag tcaaatccta caatcttaac atccttattt tttgcagatt caagtgcagc
     7141 tttagctcct agtacactat tttcagctgt tgcatatatt atatctacat ctggattaga
     7201 ttgaattaag ttttctactg tattaagaga ttcttctctg tcatatcctg gttgtgaatt
     7261 taatatctta acatttgctg aaccttttaa tgcatcttta aatccatcta ttctctgcat
     7321 ttgtatttga gattttaaat ctgtcactat tgctatttct gactttccac ctaacttttc
     7381 atctatgtat tttttagtgt attcccctaa ttttttacca gcatcataac taactgttcc
     7441 tacataactt gtaatatttt cagtatttaa tacagcatct actgtaacta ctggcatttt
     7501 ttcaaatgct ccttctactg ctggtactat agccgaatta tcagttccaa gtactatcat
     7561 tccatctaca ttttgttgta taaaatcttc tattgctgat gtttgcttag ctacatcttg
     7621 gtctgggtca tttactataa ctttcacacc taattcttta gctttttctt ttattccctc
     7681 atcaatatct ataaaaaatt gatgttgaag agttattgta gatactccaa tagttaaatc
     7741 tttcttgtct gaaccacctt tattatctga tttacttggt ccttccattg aacaccctgt
     7801 taacattcct attgctagta ttccagcaat tcctaaagat aaaatttttc tcactctcat
     7861 aattaacctc catttttaat agttttaaat ttgaacaaat tagattttta tttattattt
     7921 aaatactttt caacatcttc tttatagggc ataccagatt gtgcacctag tttagttaca
     7981 gaaagacctg atactactac tgcatattct actgctgtat ataaattttt cccttctgtt
     8041 atagccactg ccaatgctcc attaaatgta tctcctgctc ctgtggtatc ttgaacatca
     8101 acacttatac aagatacatg cttgatttcc tcaccatcat aaaatctagc tccatttttt
     8161 ccttctgtta ttacaagttt atttggatat ttttttaata cttcttctat cccttcattt
     8221 gtgtcaaaaa ctatcttata ttcatgttca tttggtgtta agtaagttac tttttctatt
     8281 atatcttcat ttagttttac tgctggtgct ggatttaata aaaccctaat cctattttca
     8341 aaacagaaat tcactacata atttattgtt tttaatggaa tctctaattg tagcaatact
     8401 atatctgcat tctttatctc ttcttcattc tttttaatta attctatatc tactagctca
     8461 ttagtccctg gtacaacaac aatactatta tcattttcac ataatgttat aacagcaaca
     8521 ccagaacttt tgtgattatt tctggatact aagctataat caactttatt cgttctaaaa
     8581 tttcttatta actcctctcc taagccatct tcccctatgc atgatatcat ttttacattt
     8641 gctcctagtt tagaagctga gatagcttga ttagcccctt ttcctccagg tgatgtaaaa
     8701 aaactattac ctaatactgt ttctcctttt tctggtcttt tatctacaga acataccatg
     8761 tccatgttta cacttcctat gacaactatg tttcccatta cattactcct ttacatatct
     8821 atttatgtct ctcgtgctgt tattcacttt cttttgttga atctcttatg accaattctg
     8881 gtttgaaaat caattcatca aacgattctt ttttatctcc tcttaaacaa tttatcaata
     8941 tttcactggc tttatatcct atatcataag acggctgttt tactgtggta agagaagggc
     9001 ttattagact tgatgtttga atatcatcaa atcccacgac acttatatca tttggtatac
     9061 ttaatcctct ttctttaaga cctcttatga cacctattgc aatcaaatca tttgcacaaa
     9121 atattgcatc aaaatctatc ttttctaaat tttttataaa ttcttgtccc cattcaatct
     9181 tatattttcc ttcaaataca taagcaggat tgtagcttaa attgcactcg attagagcct
     9241 ttttatatcc ttctaatcta tctactgcta tttcattttg aagtggacca cttaggtata
     9301 ttattttctt atttccaata tctaataaat gttttgtagc tatataggcc ccttcaaagt
     9361 tatttatttt aacaattcct tttaaggttt ctttgttcat acttatcttc ttatcaatca
     9421 agacagttgg tatttttata tctttataat ttgcttcttt ccagtttgta tttgaagatg
     9481 ctgcaaatat aattccatct atcatcctct ctgatagtgt tcttattcct tcatattctt
     9541 ttttagcatc ttcatctgta ttacataaaa ttatattgta gccttcttca ttagctttat
     9601 cttctacacc tcttgataca tctgtaaaga atggatttcg tatatctggt ataagaagcc
     9661 ctatagtatt agttttttta gttactaaac tttgtgctaa tttatttggt acataatttt
     9721 tttccttcat tattttcaaa attttttctc ttgttgcctg acttatattt tcatctttat
     9781 tattaatgac ttttgacaca gttgttttag aaacaccagc caattcgcca atttctttta
     9841 tagttatact caagaagttc acccccaaaa tttaggaaac cgttttactt aaccggttac
     9901 ctagatacta tcacatcttt ttttcatttg caaccatttt atataaattt tgatattttt
     9961 cccttttttc aaaatattgc aacttataat tcaattttaa tatttaaatt aatctgcaaa
    10021 aaaaaattaa tatactttta taattatcta aaagtatatt aattaattta agcagtattt
    10081 attaaaattc aaatccttgc tctataatca tttttttatc attttcaatc ccacttccaa
    10141 cagttgtcaa ataatcacca acaattgcag aatttactcc cccattaaat ccaactttgt
    10201 catatccctt taatgatagc cttccaccag cataccttat gtacacatta ggaattataa
    10261 acctaaatac tgctgttgtt tttagtattt ccattggttc taatacatca taattttcca
    10321 ttggtgtatt cttaatagga tttaaaatgt ttattggaaa tgattttaca tttaattctc
    10381 gtatttcaaa agccattttt agtctctgtt cttcactctc attcattcca attataccgc
    10441 ctacacatac atctagccca gcttttttag catcttttat agtttttatc ctatctttat
    10501 atgtgtgtgt tgtacatata ttatgaaaat tatctctaca cgtctcaaca ttgtggtgat
    10561 atgtagtcac acctgctgac ttcaacattt tagcttgctc ataatctata atacctaaag
    10621 atgcacataa ttttaaatta gtattttccc tcaatccttc atagatattt aaaatattat
    10681 taaactcctt tcctgacata cctttaccac ttgtaaccaa tgagaatcta tgcactcctt
    10741 tactctccat ctctttagct ctatttaaga tttcattgta atctaataat gaatattctt
    10801 ctacaccagt tttataataa gaagactgag cacaaaattt acagtcttca ctacatttgc
    10861 cagatttagc attcattatc gtacataaat ctgcttttct tcccataaaa tattctctta
    10921 tttcatttgc agattttaat agaatatcaa aatcgttttt attatttgta tcaaggctta
    10981 ttaaattaag agcttcttca taacttattt ctttttctct caatacttta ttttttaatt
    11041 ttattatgta tttttccata tatcctccta tttgtcaact taatattttt atgtagttta
    11101 cttattatat atagtattgc atatataaca tttattggtc aactttattt tagacacaag
    11161 tttactatac acaaaaaaat actgcttcaa aacaatatta gtatatagat tatacatatt
    11221 attattaaag cagtattttt tattagaata taattcttac tggtttataa aactatcctt
    11281 gtcttaactc catccaagct ttccattgta ataacttcta agccttcata ttctctaaat
    11341 agccatttta aatccttcat ggcttgatta acctgacttt ttgttagatt atttataaaa
    11401 tattcttttc tagtttcctc atactctttg ctagtcaaaa agtttcttag aaaattaaac
    11461 gcaaatacac ttggaatatt gtttacatta gccttcttgc tgttttcctt ctcatagtaa
    11521 attttcataa atgcatactc ctttgatact tttagatttc tcttacaata attatatgca
    11581 tttaagtgct actattctta taattttatt aattcatatt ctttacttcc catacccatt
    11641 tcacatgcat agtctattat tctagttcca tttacatgag gatgttcatg tttgaaaaca
    11701 tcatgaccca tttccttaca aatcaaatca tagcatgctt gttcaatagc tacagggtct
    11761 gttgatgcta atattcctat atcatgggca attggtctac ctgaccaagg tacacaatca
    11821 caaagtggag ttacattcat aacaaaagtt atatacccta ccttgtcttt tttatttgat
    11881 actgctccat aagcgtattc tgccatcttt tcgacaaaca catctgagtc actttcccat
    11941 tgtatagtta ctgctctagt tggacagaca gttggacatt ctccacatcc ataacataca
    12001 tcagaatcta taactgcttt tttatccact atacttatag cttttgtagg acaagaatta
    12061 acacactttc cacaacctac acacttcttt tcctttacta ctggagtaac atcagaatgt
    12121 tgcatttgtt ttcctgctgc acttgcacat cccatagcta agttttttaa tgctcctcca
    12181 aaacctgctg cttcatgacc tttaaaatga ctcattacta tcattgcaga agaattataa
    12241 atttctccac ctatttttac actttcaaaa tgttttttgt ctatttttac attctcataa
    12301 tttctactat ataatccatc tgcaattatt actggtgcat ttacaactgc ataagcaaat
    12361 ccattttcta tagcagttgt aagatggtct acagaatttg ttctactccc tgtatacagt
    12421 gtatttgtat ctgttaaaaa tggctttcct tcgcagtctt taaccttatc aaccacttgt
    12481 ctaactgcca caggactcat atatgtagta tttccctttt ctccaaagtg aagtttaatt
    12541 gcgacttggt catttttttc tattaaatct ttgaatcctg cttcatcaaa taatcttctt
    12601 acattgtttg gtacgttttt attttgcgaa tttgagtata aatcacaaaa atatactttt
    12661 gacttttcca tatctacacc cctttaaaat atttacttat actaatttta tcacttttaa
    12721 agtaattttt agtatatttt aacataatta aatattctta tatttatttg atacaaataa
    12781 tattatttgt attattttct acacttaatt ctatatatta aactacacct aaaaatcaaa
    12841 aagacttgaa acttaagtcc aagtcttttt gatttttagg tgtagttttt aacaacataa
    12901 gattacttta ccttgtgata aaggtattat taaactggtt ttttattaaa taaaataaca
    12961 gctgctagta aacatactat agaaataaga actgttataa taattggaat aataaattct
    13021 ggcattgcca ctttccctga aatcaagtct atattttttg acatcaagaa attaggagta
    13081 taaggtgcaa tctgtttagg aatagaaatt aaactcaata agagagttgc taccaataca
    13141 agtaaaacac tggcaaatgc tggtctaaat agtacacacc caagaattaa tacactaata
    13201 tacataattc ctataagcca taaattaaat gctgaaaata taatatgatt aagatttgca
    13261 gttggccaaa aatacattgt atagccataa gcaaataaaa aactcaacca aaaaccaatt
    13321 gtcataataa tcaccgtaac agagaattta gacaatacta tagatgaacg tgataaacct
    13381 ttagtcagca taataacaag tgtcccttta gaatattcat ttgataaaat attacaaaaa
    13441 ataattagag ttaaactcat accaagacca gagatattgc taaaaaattg tgtccaagaa
    13501 tctaaagcag ttggtgcagc agtaactttt aaagtcggtg caaaatgagc aatcaagtct
    13561 ggcataaatt ttgcagaaat aggactactt ataccaaata ttagaaataa aataattaaa
    13621 ctaaataatt tataatttct taaattctct ttaaattctt tttttgtaaa tgctatgtat
    13681 tctctcactt tactacctcc aaaaataaat tttcaagtga taattctatt agttccatct
    13741 ttactggaca aagattcatt tctgcgagtg tagatataac tgttttttgt attgctttta
    13801 tatctttacc atgcaaaaca atttccatat tagtttcttc tgaatctttt agtaaggatt
    13861 taataccatc actagactta aacttttgaa tttcatcatt acttgcaaac tccagtaaaa
    13921 gtctatcctt tccgtgcata ctttttattt ctgaaagtgt accactaagg gcaatgcttc
    13981 ctttgtttaa tatagcaaca tggtcacaga ttctttcaac atctgatagt atatgtgttg
    14041 aaaatattac agttgttgag tctttgattt taagcataat atctaatatt tcttttcgac
    14101 caacagggtc aagtgcactt gttggttcat cacaaataag taatttgggg cgtgaaagca
    14161 aagcttgagc tataccaagt cgttgcttca ttccacgaga aaacccacct attcgtctct
    14221 tttcatttcc aagacccaca agtgataaca actcttcact ccttatctgt atttcttttt
    14281 ttgaaagtcc cgtaatttca ccacaaagag aaagatattc aagtggtctc atatagttgt
    14341 aaaactctgg aacatcaggc agataaccta catgacgatt agaacttgtt ttcccatagg
    14401 tcactttctc accacaaaca tctatagttc caccatctgg tttaagtaac ccaagtacca
    14461 ttttcattgt tgtagtctta cctgcaccat tcttacctac aaacccaaag actgaccctt
    14521 ctggtacagt aaaatttaaa ttatctatta tctttcgatt accaaaacct tttgataatc
    14581 catttattga gacaatattc atcattcttc acctcttcct attgtaaaat acaaaattgg
    14641 tcctattaga ctaataaaca gtactacaat aatccatatt gccttatttc caattttata
    14701 atttggatgt ctcaatacat gaacaagtgc agtaattata agtattaaat ctaaaataat
    14761 aacaggaacc aaaagtggta agtattccat caaattttcc atactcttat tcctccaata
    14821 aagataattt ttctataaga aatttgtatt ctttgttttc acttaataca gaaaacattg
    14881 gattattttt aatagcagca acaatacctg cttttacgat tgtatcatca cgtgtaatac
    14941 ctgttccaat ttcagaaatc catctgtcaa gtttattaaa aaagtcatcc ccatgtatta
    15001 taattgggta tatatcacgt gttgctaaat ctacatattt tcgtaatgat ttaatagctc
    15061 cttcattatc accatgtatt aaattaattt gtgcctgtgt taaatatgaa gaaagcataa
    15121 tagcaggaga taatgtatca agctcaaata tatctgaaat tgctagaata cgtctttcta
    15181 tttccttcat ttgtaattta tctgcatttt gcaaaatacc cactagattt tggactaatg
    15241 atataaggtt ctgatatgca ccaagttgaa atgtttctct tgcttcatcc atttgaccat
    15301 tcatcatttg cccttgtgca agaagtatag attcatttat catgggaaaa ttgctgtttt
    15361 gcaataaatc aatcacttgt atgggttgat ttaacaatat ataacaagtt gcctccatac
    15421 tttttgcttg acggcaaata tctatgtcat tacaagtttc ctgtatacga ataaatattt
    15481 ctaatgcttc actaattaat agttcacgct ttttttcatc tacaatatca tagtgattaa
    15541 ctattaaaag acccatccga aaaatcaaag gaaaacatga ataatatttt tttatgattt
    15601 ctcttatttc atccatgact tcatcaaatg gtttaaccgt aaagtctttg cacagtttat
    15661 tatacagttt attaatatct tccttcatca tttgaggttc ataacatatt aactcatcaa
    15721 cggtaatgtt gaaatatgtc gcgagttgcg gtaaaaaggt tatgtcagga tagctttgtc
    15781 cagtttccca tttagataca gaagctttag aaacgcctat ataatttgca agttcatctt
    15841 gtgtaattcc tttttcttta cgctttaaaa tcagcgtttt tgcgatgttt aattcattca
    15901 tgtcaatccc tccttatgta taattatatc cttaaacatg taaagctaac aagcgatttg
    15961 aagttgactt ttgacgaaaa aatcaaccac agagaaacaa ataaacaagt atataaaata
    16021 tatatttaaa tttatcttta atctcctttg taaaatatgt aaactttgta aataaaatcc
    16081 atattatata aaactaatag ttaatctatc taaatacagt ttttatttat cacgttttta
    16141 aatattttgt gtaaaacctc ttttaatata aacaccttat tttcatttct catttcatat
    16201 tctttttaaa atactgaata taactcttcg attttccatt taatcctttt ataaagttat
    16261 tattcgtatt tattcctcta attataacag gcaactatca tgtattggta tttgtctatt
    16321 atataaaaat ctataaaaga aaagcagtca atcatttaga ttaactgctt gtacatatat
    16381 cttattaaac tgtataaact ataattttta ttattctcca ttttcatccc aagtcataaa
    16441 tccccatcca ctatctggat aaatatcgat agacttttct ctaatcatat aaccaacttt
    16501 ataacactct tttgttattt tattagatag tatacatcct tctccattta tctaattcca
    16561 tttaattaac ttttcttttc aaccttaact tttataaaat catctttttt aataatttca
    16621 tacttatttt ctcttcaaca tcatcaaatg agggtgtctc aaaattaact ttttacttca
    16681 taaggcactc ttttttgtat gaagtaaaat atattttcat catttcaaca ataagtaaga
    16741 ggcttatctc tattttgaga cagccccatt tgatggtgtt gaacatttaa ataaggttga
    16801 actcgtttta tctctctttt taagatatct gtaaatttgc acataaagtc tcttcttctt
    16861 ttacaattct aatagtttta ttaaagccaa agtatatatc tccatattct ttcgtatatg
    16921 atttattggt actctttcat ctactccatg accaccagtt ttatacgcta agaacttagg
    16981 tccaaacgcc accatatctt taaaccaact tgcatatgtg ccaccaccca tagcaattgt
    17041 atatccattt tcatctgtta cttcttcata ggcttctaat aattttttaa gtactaaatt
    17101 actctcatcc acataatgta agtctttatg agcaataacc tctattttcc aaccattaca
    17161 aaaaaccttt ttgaatctat ctattatatt gtttaaaact atatttttac cataccttat
    17221 atctaactca catacgattt tttcctcatt tacttttaat attcctaaat ttaaggtagt
    17281 gtttttgaat acctcatcat attgattaat ccccaatgca tctccataat aatcactaca
    17341 aaggtatttt gaaactaatt ctatatcttc tctatgctga aaatccatat tatcctcaac
    17401 tatctttatt accattttag atatagcatt ttctcccttt tcaggattac ttgcatgtac
    17461 agctactcct ttagtagtta actctgaaat aattccatta ataatttttg cagtacaatg
    17521 agatggaaca acattttctt tagttcctcc acttactatt atgtaaggtt tacttttgtc
    17581 aattttaatt tctttttcta tccttatatg aacccttcct ttttctccat taaccactgg
    17641 atacatagca tctggagtaa atcccatact tggaatttct tcacctttat ctaagtaata
    17701 tttgatacat ttcatacctg tctcctcatt tgtaccaaaa attaatctta ttcttttatt
    17761 aattgtagga tacatatcct ctatgttttt caatacatgt ataagcatag atacagcacc
    17821 cttattatct atgacacctc ttccataaat aatattgtca attactgctc ccgaaaatgg
    17881 gtctacactc cacccctctc ctggtggaac tatatctaaa tgcattggaa tccctattaa
    17941 ttcctctccc tctccaattt ctgcatatcc aatataatta tcaatatttt taacactaaa
    18001 acctaaacta gcacagtaat ctaaagtttt atctaaagat tttttaggtc ctataccaaa
    18061 aggacattgt gaaataggtt caccttgttg tgaatcaatt tttatcaatt cttttagaaa
    18121 ttctatggtt ctatttaaac tattatctac acttctgctt atatccataa atcaacctac
    18181 tttctatctt tttaaagcct gtccttgaag aacaggcttt tagcattaaa aattattttt
    18241 ttgtaaatac taattcacaa acttcatcat catgagcaca tttttgagca aaatctaatt
    18301 taacaccttg tgcacagtca actcttccat aatccccata acaagctaat ctacaaattt
    18361 cttttctttc ttctttagtt aatccatatt ctttccaagc agcatctaat ggacagtgat
    18421 ggaattttaa aacgccattc tcatctgtat tttctactaa ttccatttca aacacttttt
    18481 gtcctttact gttatataac atttctgcca tttttcctgg agtatctgct acgttatatt
    18541 tttgacctct cattttacca aatccatata ttgcttcttt acatatatca tctggattag
    18601 ctcctaattt ttcagcttct ttcataagaa gatagaacca tactgctctt tctcctgttg
    18661 ctcctctaat tccttctaaa tattcctttt ctgtatacat tttttctgac atttctttct
    18721 cctcctaaaa ttaaattcta tattttaatc actgtgaatt agaatattcc aatgaatgac
    18781 cctaccaatg ctaatacaac taaaattact aataacttag ttattgtcca cttcttgtat
    18841 ttcattcccc aatatacgaa gaatacaaca ccaagtggta ataaacctgg agcaatcata
    18901 tttaatgtat cttgaacaac aattgcttta ccaccaatat caaatgatag cggagttgaa
    18961 agcttaactg tagttgcaga taatgctccc atcataaaca ttcctagaat tgaagcacca
    19021 tcaattatct tattgacaat accacctgat agaattttct taattgaatc tctacctaaa
    19081 gaatatccta aatgaaataa gtaataacct tcgcaaaata gtaatatact aaacattatt
    19141 gggaaaaatg cacctataac acttccttct gctccaaatg aagcacctaa agctaagaat
    19201 attgtttgaa atgtaccaaa atctaaagta tctccaattc cagccaaagg tcccataaga
    19261 ccattcttaa cacctgaaat aacttctcct gggatttttc cttcagtagc tttctcttct
    19321 tccatagcta aaactgtacc gtgaattaat cctccccata tagcttgagt attaaaaaat
    19381 tgtaagtgtc tctttaacgc atctactaaa tcttcttttc tcttatataa cttttctaaa
    19441 gccggagtga aacttgcaca aactgctaat gcttgcattc tttcaaaaga gttggataat
    19501 tctgctgtcc accaccatct tagatatgtt tttgttacat ctttctttgt aagtactctt
    19561 gtcttattaa cttcagttgg accttgtcca tttttaactt ctttattttt tatttcttgc
    19621 attatacttc acctaccctt ttgtcttctt tcataaaggt aattaaaaga gctatacaaa
    19681 caccaaatat tgcagctgca gtaattgata tttcaaaata ttgaaccata aaaaatccta
    19741 taataaacat tggtaaaaat ttatttttac ctatagtaaa tattgttgtt gcaaaaccta
    19801 atgcaggtaa tataccgccc attactgtta atccattcat taaccattgt ggtataacat
    19861 ctaataatga ttgaactaaa tctgctccaa aaaagttaca tacaaaaact attggaaacc
    19921 ttaaaacaaa cccaaatatt aaagaataaa gtgttgcaca tctccagatg ccttttgtat
    19981 taccttcttc agcatactta tctgccttat gaaccaatac agcattacca gttcttctta
    20041 agttattaac taatactcct ataacaccta aaggtaccgc aatagatact gcaacttctg
    20101 cattaacacc agtttgaagt gctactggta ttgcaataag agcagctaga catttatctg
    20161 atggaatatt tccccctgct gcgaccattc ctagataaac catttctata cttgcaccaa
    20221 caatcatacc agttgttaca tcaccccaaa gtaatccaag gctaaaacct ataaataaag
    20281 gagactttag tgttgagaaa agggtatacc ctgcaagacc tgctgcaaac caataccata
    20341 atcctgttac aattgccaac atcaaaacag attccattta taattcctcc taatttatat
    20401 tttttattta aatttcttta acgcattttc atatgtcata gatgattcct ctggtataac
    20461 ttgtaggact atttcaaccc cattttcatt aagttcatta agttgttcta catcctcatt
    20521 tcctaatgaa acagctgtaa acaccatttt ctttcctgct tcatttggaa tacctcctaa
    20581 ttgaatcttt tcatatttaa gtccagcttt ataagcctta tttgccatat caacattttt
    20641 aaatagaata aatatatttt tatctcctaa agtgttgttt tggaatgctt gtcccgcatc
    20701 ttctgctgtt aaaataacta cttctactcc tgatggtgct gccattttat atatgtcagc
    20761 cataaattca tcttgaccta agaagtcatc aactattatt attttttgag cttgagcgat
    20821 ttttacccat tttgttataa cttgcccatg aattaatctg tggtctactc ttactaatgt
    20881 tatttttgcc attatattca cctcataatt tatatttttt taaattcttt aattaaatcc
    20941 acacaaaggt tattattttt tttgacgatt ctttctggta atgtatgtaa tttatcttct
    21001 ttgcttaatt cttcatctac gattaaaatt gtctgtaagc taagtccatt tattgcatct
    21061 attcctcttc ttatagcaac agctattgca acacttgatg tagatcctcc atataaatct
    21121 gatattataa ttttcacatc ttctttatct actacttctt caacctcttt cataaataca
    21181 gaaacatctt tttcagaact tagtgataaa acttcatatt gatttgacaa tccaaagttt
    21241 ttatttgttt cttctactaa gacttttccc caagttccat ggcacagtat gattacctta
    21301 tatttattca ttgctaaata aactctcctt tatagcgatt aatattttgg tttaattcta
    21361 cttcttcttc atcaggtacc atttgaattg atacattaat attcttttta ataaattcat
    21421 ctattaaaag aaactctttt ttattaatgt atacattttg agatattttc ttttttccta
    21481 ttcctgtagg tattcttcca acaataagtt ttttgatatt aatcccttct tctatcattc
    21541 tatttgcagt atctaaattt ttaattaata caaaaacgtt atcatcagct tgattattat
    21601 aaaaatatct ccttgattca cttacagtta atattgataa atcaatattc tttggtgctg
    21661 ccatttttat caaaccttta gcaataacat cattggttgt ttcatcatct actactacta
    21721 tcttttttac acctagatgc ttaacccatt tatatataac ttgaccatgt attaatctat
    21781 catcacatct gcaatatttt actaaagaca tttttaatca aactcctttt tctattacat
    21841 tgagctaaga actttattaa tattgtttat accagtttgt cctgcttcaa tacatgcttc
    21901 tgctaactct tctaaacttt ttccctctct attaattaaa gcttcaagta gcattggaag
    21961 atttactcca gtaagtgcat gaaacttgcc acctttttta aggtttaaac ttgcaacatt
    22021 acttggcgaa cctcctaata ggtcagctaa tactaaaact ccgtcacctt catctaattc
    22081 atttaacttt tctacaaaat ttttatttaa gtcttcaata ttatcattcc tatttagttt
    22141 aattgctgct gttctttctg ctggtcctgc aataagttca cagcttttta taatttcttc
    22201 actaaaactc ccatgagtca taactattat tcctatcacc aatttcacct cacttttctt
    22261 gtcacttttt atataagcaa ttgccgtgcc aacttatgtg tatcaataac aaatggctat
    22321 tttacaggat ttaatcaaaa ctttttactc aatttttttt aaaaacttat tatttcgata
    22381 tgtatcaaaa gattttttca acctgtatca aatctgtatc aaaaaaagta aaaaaaagat
    22441 agccttttgg ctatttttaa attttgtatc aacttttgaa tttatctgta tcatattgtg
    22501 attcaaatat atctaatata tttgcatatt ccatatctga aataattata tcgaatgtct
    22561 cttctactag cttaaaactt tcttttataa ctaaatataa ttctttattt ctactaattc
    22621 tttcttcata tgatttataa actaaagact cttttattat tactctttca atcatacatg
    22681 aacaatgaaa tataaacttt actaaaagtt catcttgaat tactaaagaa tagtgttcac
    22741 taattctatt aattacttct tttaatataa aataagcctt ttccccattt aaaaaagtca
    22801 aagttttact aatattatca attagtaatc tatcaaaata tctaccacca ttatcttttt
    22861 cagcatatgt aacttccata tctccataat gtttctttat agattctata atattaaaat
    22921 ataatttctc taatgtcatt tctggagtta gagcctttct agttgcttct attacaatag
    22981 gtgtacttac catatctatt gcttttactt cttttcctgt agcatcacga ataactttag
    23041 caaatgttaa aatagacccc atatcagtta aaattaaaat tccttttcct ttatcaattt
    23101 tattggctat ttctacagct tgtactaaaa tatcctgaac tctatcttct aatcccatat
    23161 taattgccac tgcatggtta caatccagta aagtattggc tacattcacc atgctagatg
    23221 ctgtagattc accatgcatt atgactaata ttgctatact ctcctcattt aatcccacat
    23281 acgataaata aagaaatgtt gctaaaaaaa ttgcttcatc ttctggaaac tctactccat
    23341 acattaatga catgttagat aatatttctt ttgctatgtt atattcatat gctctttcct
    23401 tcataatttc atctttatta ggatgcttta ctatatttcc aatctttatt ctttcaatta
    23461 aattctgaat atgtaaagct atagctttta ttaatttatc taatccttct cttttgctcc
    23521 atttatcatg cttttgtaaa atatatttta cagtagttgc aattgaatca ttaacaatgt
    23581 tgttataagc t
//
LOCUS       DAELLV010000033        23423 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.033, whole genome shotgun sequence.
ACCESSION   DAELLV010000033 DAELLV010000000
VERSION     DAELLV010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23423
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.033"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(201..1451)
                     /locus_tag="KPD58_001128"
     CDS             complement(201..1451)
                     /locus_tag="KPD58_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HBG0264911.1"
                     /translation="MATIKPFKAIRPNKYIVDKVAALPYDVMNSKEARRIAEGNPYSF
                     LHIDKSEIDLDENIDLYDEKVYLKARENLDEFRKQEILVKDDKECIYIYKQIMDGRAQ
                     VGIVACISVDESLNGTIKKHEYTRPEKEIDRTKHIKYCDANTGTILVTYKHQRVIDDI
                     INDFMDNNEPLYDFITDDKIEHTVWKIDNDDIIDNLVDKFEKLDYLYIADGHHRTASA
                     ENVAKEMRAKNPNYTGKEEFNYFIAMIAPDENLMVLDYNRVIKDLNGLSEEEFINKIK
                     ENFELEEIEGKEKYKPDKKGTFGMYLGDKWYKMKANKNLLEIEDPVDSLDISILQDYV
                     IDAILGIDNPRVDKRIDFIGGIRGLEELEKRVNEDMKVAFAMYPTSIEDLIRVADANK
                     IMPAKSTWFEPKVRCGLFLHEINE"
     gene            complement(1483..2424)
                     /locus_tag="KPD58_001129"
     CDS             complement(1483..2424)
                     /locus_tag="KPD58_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HBG0264912.1"
                     /translation="MYNILVTDGIEKEAARKLRELDFNVIEQFYEKDVLGDKLKDVDV
                     LVVRSATKVTKDVIDKALEGKKLKLIVRGGVGLDNIDVKYAQANGIKVMNTPNASSIS
                     VAELTIGQLFVLARFINTANVTMRDGKWEKKKYKGTEINGKTLGLIGFGRIAKEVAKR
                     AELLGMNVIYTDIMGEAQGFNNYKFCDMEEVLENADFLSLHIPFDKNKGAVITEKEIN
                     KMKKGAYLINCARGGLVDEKDLLKALDEGKLSAAAIDVYEQEPTLNLDLVNHPRVSPT
                     PHIGASTVEAQERIGDEIVNVIQDFFLDFNNLIGVAL"
     gene            complement(2664..3740)
                     /locus_tag="KPD58_001130"
     CDS             complement(2664..3740)
                     /locus_tag="KPD58_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBG0264913.1"
                     /translation="MSKKLFIPGPIDVKEEVLQKMATPMIGHRGKDASMLQKSISEKM
                     QKLFYTNNTILLSTSSGTGLMEGSIRSCTSKKAAVFSCGSFGDRWYKMAVANNVPADI
                     FKVELGEATTPEMVDKVLSTGEYDLITVTHNETSTGIRNPIEEIGEVVKKYEDVIYCV
                     DTVSSAGGIKVEVDKIGIDICITSVQKALGLPPGMSICTFSQKAIDRAKQVPFRGVYL
                     DLLAMYEYLIKKNYQYPSTPSLSHMFALDFQLDNILDEGLDNRFNRHEDMANLVRNWA
                     KKHFQIFTNENHLSNTLTVIENTQGISVSNLNSKLQERGFQIANGYGDLKEKTFRISH
                     MGDYTVEDVQELLDNIDDILGFNK"
     gene            complement(4113..4628)
                     /locus_tag="KPD58_001131"
     CDS             complement(4113..4628)
                     /locus_tag="KPD58_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HBG0264914.1"
                     /translation="MDFLAKYKVLKKQNSSKSCLVCGTQNELGLKADFYELENGELVS
                     ICNTKDWHQSYPGRVHGGMSAAILDETIGRAVSINDDQIWGVTVSLELKYKKPVPTDA
                     TIKVVGRITKENRKLFEGTGEIILPNGDIAVTATGKYMKMPIGQIAEGDFSNEEWFFE
                     ESKEKVEYIEL"
     gene            complement(4991..6061)
                     /gene="leuB"
                     /locus_tag="KPD58_001132"
     CDS             complement(4991..6061)
                     /gene="leuB"
                     /locus_tag="KPD58_001132"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HBG0264915.1"
                     /translation="MNCNIAVIKGDGVGPEIIDEGIKVLNKICCKFNHRFDCEYVLAG
                     GCAIDETGEPLPNKTVETCRKNEAVLLGAVGGPKWDKCKGDKRPESGLLKLRESLGLF
                     ANLRPATMYESIKEASPLRTDIVEKGIDFVVVRELTGGIYFGERGRKIIDGIENAYDV
                     EIYNENEIRRIGKRAFEIARNRNKKLISVDKANVLESSRLWRSIMEDLAKEFEDVELS
                     HMYVDNAAMQVVKDPSQFDVIVTNNIFGDIISDEASMITGSIGMLPSASLREDSFGMY
                     EPIHGSAPDIAGKDIVNPIATILSVSMMLRHSFNLEEEAKCIEDAVQSVLNKGYRTID
                     IYNGVGNVVGTREMGELIVNEI"
     gene            complement(6068..6559)
                     /gene="leuD"
                     /locus_tag="KPD58_001133"
     CDS             complement(6068..6559)
                     /gene="leuD"
                     /locus_tag="KPD58_001133"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HBG0264916.1"
                     /translation="MIANGSVFKFGDNIDTDVIIPARYLNIADYKELATHCMEDIDDK
                     FISKVKKGDIIVATKNFGCGSSREHAPIVIKESGVSCVIASTFARIFFRNSINIGLPI
                     LECEEAANNIDEGDNIEVDFSTGVIKNITKGKEYKAEPFPEFMQNIILNEGLINSIKA
                     NRG"
     gene            complement(6574..7851)
                     /gene="leuC"
                     /locus_tag="KPD58_001134"
     CDS             complement(6574..7851)
                     /gene="leuC"
                     /locus_tag="KPD58_001134"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HBG0264917.1"
                     /translation="MGMTMTQKILAKHAKLNEVKKGQLIEADLDVVLGNDITSPVAIR
                     EFEKLGIEDVYDKTKVVMVLDHFTPNKDIKSAEQCKFTRSFAKSKGVVNFFDVGDMGI
                     EHVLLPEKGIVTAGDVIIGADSHTCTYGALGAFSTGVGSTDMGAGMATGKCWFKVPGA
                     IKFVLKNKPNKWISGKDIILHIIGEIGVDGALYKSMEFCGDGVEYLSMDDRFTICNMA
                     IEAGAKNGIFPVDDKTMEYINSHKCSTMTKDVNIYEADEDAVYDEVYEIDLAELKETV
                     AFPHLPENTRTVDEIDKDIKIDQVVIGSCTNGRISDLEVVAEIMKGKKVADGVRVMIF
                     PGTQKVYLEAIEKGYITTFIEAGAAVSTPTCGPCLGGHMGILAAGEKSISTTNRNFVG
                     RMGHVDSEVYLASPAVAAASAITGKISKPSEII"
     gene            complement(7808..9550)
                     /gene="leuA"
                     /locus_tag="KPD58_001135"
     CDS             complement(7808..9550)
                     /gene="leuA"
                     /locus_tag="KPD58_001135"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HBG0264918.1"
                     /translation="MKCGKYKKYDKMQIVNRKWPDNEIFKAPIWCSVDLRDGNQSLPT
                     PMSVNEKVRMFKMLIDTGFKEIEVGFPSASNTEYTFLRKLIDENMIPDDVTIQVLTQS
                     RAHLIEKTFESIRGCKKAIIHLYNSTSVLQRDVVFNMSKQEIIDIAVEGAKLFNEEVK
                     KYPETEFTFEYSPESFTGTEMDYALEICEAVIDVWKPTPQKKVIINLPSTVEMATPNV
                     YADQIEWFCKNISCRDSIILSLHTHNDRGTCTAASELGLLAGADRLEGTLFGNGERTG
                     NMDIVNVGLNLYTQGIDPELDFSNIDKIIGIYEDCTKLMVHDRHPYAGNLVHCAFSGS
                     HQDAIRKGMIAMKNRDNDYWEVPYLPIDPHDIGREYKEIIRINSQSGKGGAVYIMETD
                     YGFMIPKNMHSDFGNVVKMESDRIGEELSSEAIFNLFKKEYIEVESPYKVKKYKIKSM
                     DELNYENDDSNDTNMIEMTARISYMGNEQRIVGIGNGPVDSFNNALKQCGMKDYKFRY
                     YWEHALEEGSHSRGVAYVGIEHNNEVYFGVSISENINTAAINALMNAINKSYIEEEIK
                     NGDDYDAENISQTC"
     misc_binding    complement(9674..9920)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(10068..10514)
                     /locus_tag="KPD58_001136"
     CDS             complement(10068..10514)
                     /locus_tag="KPD58_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264919.1"
                     /translation="MSKNKFIYMNYYDNGYFKKGMTIYMVLALIILATIFIYSTTILY
                     ILCIITAVFLFKQGRDFSNKYEGKVYITLDNHSILINNQCIFSIKSKQSQKFNYKIIE
                     KIEVVKNILNIYTDESNYKIRLRALSLEDEKKLLNIIDEKMKKFKA"
     gene            complement(10535..11254)
                     /locus_tag="KPD58_001137"
     CDS             complement(10535..11254)
                     /locus_tag="KPD58_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HBG0264920.1"
                     /translation="MKKSIMDVHCHTLISGHAHSTFKENVEEASKKNIKYLGISDHGP
                     NMPGGPHPFYFYNLHLLPREVQGVKILRGIEGNIMDYNGNLDVQEDMLKHLDYIIASL
                     HRPCIASGTKEKNTNAILKVMDKPKVKIIGHPDDSRYPLDYEPIVKKAKDKNILLEIN
                     NSSLSSNSHRTGTWENVSHMLTLCKTYGARVILGTDSHICYSIGEFENAEKVLEAVDF
                     PDELVINYHEDEIIEFFDINF"
     gene            complement(11372..12646)
                     /locus_tag="KPD58_001138"
     CDS             complement(11372..12646)
                     /locus_tag="KPD58_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HBG0264921.1"
                     /translation="MFIMENDLTMQELLDQQEQVFSKVKVGELTTGKITAVRNDEVQL
                     GLDYGFDGIIPISELNIEKNQYIEDIYHIGDEITAVITKVSQKDGTITLSKLQLDKRN
                     DFAKLQKAYDEHRIITVNVEKNIDKGVFANYNTYTFFIPISQLDTKFITDTSKFVGLN
                     LEVYIKELDVRKNRLVASHRDVLQERINKEREERRAQIKAEKEAERARIKQEREEEKA
                     RIKAAKEDLFNSLEVGQKRDGKVTKIMPYGAFVDIGGIEGLAHINNLAWTRVESVEDV
                     VSEGQEVEVYVLDVNKETKKIALALKDINNDPWDLIAKEVQIDDVVNAKVLRIIEKGA
                     FVQIKEGVDAYLPISELSDTRVAKVTNVVNIGDEVKVKILDFKPKTKRMLVSIKEATR
                     EPEEDITEYLEVEESLGSIGELFKDKFKDLEV"
     gene            complement(12846..14213)
                     /locus_tag="KPD58_001139"
     CDS             complement(12846..14213)
                     /locus_tag="KPD58_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG0264922.1"
                     /translation="MENSNTNLGSESVGKLLFKLATPAIIAQIVNVLYNIVDRIFIGR
                     MENGEVAMAGVGVAFPIIIIITACSYLIGMGGGPLAAIKMGEQNNDEAEKIMSNSFSV
                     LVILAILLTIGFKIGKEPLLWMFGASESTIGYSMDYLNIYLIGTVFVQISMGMNTFIN
                     TQGFATTGMMTVAIGALINIILDPIFIFGFNMGVKGAALATIIAQGVSAIWVLMFLFG
                     KKSILKIKKKYMIPKASIILPVLGLGISPFIMQSTESLVLIALNSKLQMYGGDLAVGS
                     MAIMSSIMQILMLPNMGVTQGAQPIISYNYGSGQLDRVKKTFKLCLLSCFTYSTILWL
                     LLMIFPAFFVSIFNKNPQLLSMTSWSIKIYFAGAFMFGIQIACQQTFLALGKATISLV
                     LALLRKIVLLIPLIFILPTFFNEKLFAVILAEPVADITAATITAISFFIFYKCFLSKP
                     KAIKE"
     gene            complement(14463..14927)
                     /locus_tag="KPD58_001140"
     CDS             complement(14463..14927)
                     /locus_tag="KPD58_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HBG0264923.1"
                     /translation="MSLRIGGNSLGYLHILILSMVPVTELRGAIPIGIAMGLNPIWVY
                     VFSVIGSTLVSIPLILTFRHVLQFLRGKKLFKGIADVIDRKINSRMKKLKSVSIIGII
                     LFVGIPLPTTGTWTASAIASILKMRIKDAFMGVFLGNLLSGVIVSALSLHII"
     gene            complement(14932..15561)
                     /locus_tag="KPD58_001141"
     CDS             complement(14932..15561)
                     /locus_tag="KPD58_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HBG0264924.1"
                     /translation="MKKSVLLLDVDYTVINTDSMIDFFIYSLKNKTFKTIIKLPYIIF
                     ILFMYLIRMIPLKKAKEAIFYPIVDFSEEDLKKFFDDCIMKKINESMKKVIYKNKEED
                     NVIIMITASPYAYMKYFKYYGLADEVIGTEFFYENSRYKNQFIGENCKGIEKLKRIKA
                     VLGKLGIEIDYENSYAYSDSKSDLPMLSLTKNAFLVSKKDGEVIEQINS"
     gene            complement(15595..16482)
                     /locus_tag="KPD58_001142"
     CDS             complement(15595..16482)
                     /locus_tag="KPD58_001142"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HBG0264925.1"
                     /translation="MKQTNTSIRLLTKISDYIKLMRIKQYIKNFFVFSAIIFSNNILN
                     INLFLNTFIAFVCFCLMSSSVYALNDAIDMDKDKKHPKKCNRPVASGRISKKSANILF
                     VVLALISVCLSTVVSFNLSIILSLYLINNILYSLKLKNIILLDVFSISLGFILRVYAG
                     CVAISVSLSNWIILCTFFLSLYLALGKRKKEIETLRDDAVEHRKILEDYDIENLNQMM
                     IVILSSTIVCYALYSTSNPEKPHMIFTTIFVVYGVLRYNYIINTTNENNPTDIVLKDN
                     ALKINVILWIITCLIILIF"
     gene            complement(16511..17749)
                     /locus_tag="KPD58_001143"
     CDS             complement(16511..17749)
                     /locus_tag="KPD58_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264926.1"
                     /translation="MDGDILMSKILKYKNEMFLFILVVITYLIITKIFFSKTTIFYDL
                     NNTYDVLLDTDTGVLFNLNVFAISQDNSKHILFSAIISIFAYPIYLFCTSIANPGTTD
                     FNSAYGFGLICLQIITSAMSITLVFNHIKKIKMQRLTLILLTMIMIFSFPQLFMTLNV
                     ERFIYSQFSLIFFIVIANKMKGKNSYLVELAAIPLFGITISNIYLYFFNMIFEFKLKI
                     GKMLKHLITFILMAYICVVSTKSYESFMNLGNVIQYDTKFISGEPILEKIAMIIERLL
                     YSVFYFPGASIKKGLFLQNGEVATIPVILTLLALCFCVLSVIENSEKRVPKLCMGIII
                     FNLTLHGIVGYNLVNSSIMAINFSFAVIILLAYFTKALRKNEKNMYNIFLSLLLVTIV
                     ISNVNGFIEILNIGIKSYPV"
     gene            complement(18133..19275)
                     /locus_tag="KPD58_001144"
     CDS             complement(18133..19275)
                     /locus_tag="KPD58_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBG0264927.1"
                     /translation="MRYVEYGKTGKMVSVVGYGGLRFDLEKSNQENADLVKYAYEKGI
                     NYFDTAPGYCDDRSEDIFGLAFRQMIKEGKTDFYVSTKEKPKACDTSEKAIEAVKKSI
                     ERLGVSKINFYHVWNIRKMEHYELAMRPGGQYEGLLKCKEEGLIDHIVFSSHQPGDEV
                     IDILSENKFEGVTMGINILNFPYRLKGAKYAVDNGYGVVAMNPLSGGTIPKYNKELSF
                     LSKDGESTIESALRFNIGISQISISLIGFNKKQDIDDACKIADENRIYSDEDIHDIET
                     RLNKNMNEICTGCGYCKVCPKGINTPAYMLFYNEKQMFKKSDEEMTKLVYGLGHWNYT
                     MNSKAKAKECISCGKCEVECTQHLPIIDRLKEIKKWEEDGANSVKV"
     gene            complement(19416..20282)
                     /locus_tag="KPD58_001145"
     CDS             complement(19416..20282)
                     /locus_tag="KPD58_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBG0264928.1"
                     /translation="MDNYKKVKQVLWIILFANFAVALLKIIIGNQIKSYSMTADGFHS
                     LSDGASNIVGLIGIFFASKPKDKNHPYGHKKFEIITSLFISGMLFVIAIKIILSAVLR
                     IANPVVPAITIESLIALIITLFINIFVCMYEYRIGTKLNSYVLISDSLHTRSDIFVSL
                     GVLVTLVGVKLGFPVIIESIVPIIISAFIIYSAYGIFRPSIGILVDRVAVDEDYIKEI
                     VFEFNEVRDVHNIRSRGSKSSIYIDMHVMVDPFISVEQSHDLTHKIEKQIQEEINENA
                     QVIVHIEPFYSF"
     gene            complement(20616..22163)
                     /locus_tag="KPD58_001146"
     CDS             complement(20616..22163)
                     /locus_tag="KPD58_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBG0264929.1"
                     /translation="MANFFNFILLQKDKIIELLIQHMSLTVTSILIAIIVGVPLGIII
                     SRISSLRKFVLGFVNLVQAVPSMALLGLLVPILGIGSKPAIFMVVVYSLLPIVKNTYI
                     GITSIDPVVLESAKGIGLTRNQTLFKIQFPLALPIIMGGVRISAVTAVGLMTLAAFIG
                     AGGLGYLVFSGVQTVNNNMILAGAIPSCIIALLVDYLFSKIEVAVTPKGLNPKAPKKN
                     YIALKVISVVVVVSMLFVVFSSSFSSKKDTITIGSKDYTEQLILGNVYAELVEKNTNL
                     KVKKNLNLGGSSVAFNAIKSGELDMYVDYTGTLLVNVMKHAPIKDADEAYNVVKDTME
                     KENQLTLLDPLGFNNTYTLAMMPETAEKYGINTISDLTKYGKEFTFSPTLEFENREDG
                     LVGLSRDYGLKFKDVKAMNGSLRYTALDNNESQVIDAFLTDGLIKKFNLKILEDDKNF
                     FVPYYAAPLVREDTLKKYPELEKVMNMLSGKINEETMRELNYQVDELGKSPEEVAHSF
                     LVKEGLV"
     gene            complement(22156..23292)
                     /locus_tag="KPD58_001147"
     CDS             complement(22156..23292)
                     /locus_tag="KPD58_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0264930.1"
                     /translation="MIEIRNVTKKIGNNVILDDISLVVETGTLVVLIGSSGCGKTTTL
                     KLINKLIKPTSGEIYINGKPISQENEIELRRKIGYVIQNTGLFPHLTIKENIELIPRL
                     KKEKSVEEIEKRTLQLLEMVGLDSDEFLNKYPSELSGGQQQRIGVARAIATDAEIILM
                     DEPFSALDPITRTSLQEQLFSLQDELKKTIIFVTHDMDEALKIADKICIMKDGRIAQY
                     DTPENILRKPANDFVKDFIGEDRVWDNPEYIKARDIMIKNPIAVNSTRTVTQGIEIMR
                     TSKVDSLLIIDRAKTLKGIVTVKDMKDIDDKSILLADIMSSEPLHVNEGDNLVEILNV
                     MNRNSVGYIPVISDENKLVGLITRSSLLSVLSEQFLEMEVSVLG"
BASE COUNT         7610 a         4262 c         2436 g         9115 t
ORIGIN      
        1 atacaaatac aattatatga attttcatcc tcaattatag catagaatat gactatctat
       61 atcctaaaat tatccatttc taaaaaaaag gtaactatta ttaagaatat caacttatag
      121 aatttatatt ccacatacga ttttcaatca aaaaaacaag ggctaaaaag tgaattgtgc
      181 actttttagc cctttattaa ttattcattt atttcatgta agaatagacc acatcttact
      241 ttaggttcaa accatgttga cttagctggc attatcttat ttgcatctgc tactcttatt
      301 aaatcttcaa tactagtagg atacattgca aatgctactt tcatatcttc attaactctt
      361 ttttctaatt cttcaagacc tctaattcca ccgataaaat caattctttt atctactcta
      421 ggattatcaa ttcctaaaat agcatcaata acatagtctt gtaatataga aatgtctaat
      481 gagtcaacag ggtcttctat ttctaataaa tttttatttg ctttcatttt ataccactta
      541 tcacctaggt acattccgaa agttcctttt ttgtcaggtt tatatttttc ttttccttct
      601 atttcttcta gttcaaaatt ttcttttatt ttatttataa attcttcctc gcttaaacca
      661 tttaagtctt tgataaccct attataatcc aaaaccatta aattctcatc tggtgcaatc
      721 attgctatga agtaattaaa ttcttctttt ccagtataat ttggattttt agctctcatt
      781 tctttggcta cattttctgc tgaagctgtc ctatgatgac catctgcaat gtatagataa
      841 tctaattttt caaatttatc tactaggtta tctattatat catcattatc aatcttccat
      901 actgtatgct ctatcttgtc atctgttatg aaatcatata aaggttcatt attatccata
      961 aaatcattga ttatatcatc tatcaccctt tgatgtttgt aagttacaag tatagttccc
     1021 gtattagcat cacagtattt tatgtgttta gttctatcaa tttccttttc tggtcgagtg
     1081 tactcatgtt tttttattgt tccattcaag ctttcatcaa ctgatataca tgctactata
     1141 ccaacttgag ctctaccatc catgatttgt ttatatatat atatacactc tttgtcatct
     1201 tttactagaa tttcttgttt tcgaaactca tctaaatttt ctcgcgcttt taaataaact
     1261 ttttcatcat ataaatctat attttcatct aaatcaattt ctgatttatc tatatgcaaa
     1321 aatgaatatg gattcccttc tgcaattctt ctagcttctt tactattcat aacatcgtaa
     1381 ggaagtgctg ctactttatc tactatatat ttatttggtc taatcgcctt aaatggttta
     1441 atcgttgcca ttactaattt cctcctaatt atacaaaata ttttataatg ctacccctat
     1501 tagattgttg aagtcaagaa agaaatcttg aatcacattt actatttcgt ctcctattct
     1561 ttcttgagct tctactgtag atgccccaat atgaggtgta ggagaaactc ttggatgatt
     1621 tactaagtct aaatttaatg taggttcttg ttcataaaca tctatagcag ctgctgataa
     1681 tttaccttca tctaatgctt ttagtaaatc tttttcatca actaaaccgc ctcttgcaca
     1741 gtttattaaa taagcccctt tcttcatctt atttatttct ttttcagtta ttactgcccc
     1801 cttattctta tcaaatggta tgtgtaaaga taagaaatct gcattttcta aaacttcttc
     1861 catatcacag aatttataat tgttaaagcc ttgcgcctca cccattatgt cagtatatat
     1921 aacattcata cctaataatt ctgctctctt tgcaacttct tttgcaattc ttccaaaacc
     1981 tataagtcca agtgtctttc cattaatttc tgtaccttta tactttttct tctcccattt
     2041 tccatctctc atagttacat ttgcagtatt tatgaatctt gcaagaacaa ataattgacc
     2101 tattgtaagc tctgcaacag atatactgct tgcatttgga gtattcataa cttttatgcc
     2161 attagcttga gcatatttaa catctatatt atccagacct actccacctc ttactatcaa
     2221 cttcaacttt ttaccctcta atgctttatc tattacatct ttagtaactt tagtagctga
     2281 acgtactaca agtacatcaa catctttaag tttatctcct aaaacatctt tttcataaaa
     2341 ctgctctatc acattaaaat ctaactctct caattttctc gctgcttctt tctctattcc
     2401 atcagtaact aatatattat acataacaaa ctcctccaat ttttattcac ttaatttttc
     2461 caatttttat tttttaaagt attttcatca ataaaaaact ttacttaatt caactttctg
     2521 tttatataat tttcaaaata tttttttatt aaagtcatgt caaatttttc tattgtttgt
     2581 taaaaattta atatgtaatt tttataaaat attatttatt atatattcta aatttatctt
     2641 aaaacataca tatttttaca atattattta ttaaaaccta atatatcatc aatattatct
     2701 aataattctt gtacatcttc gacagtatag tcacccatat gtgaaattct aaaagttttt
     2761 tcttttaaat ctccatagcc atttgctatt tggaatcctc gttcttgtaa ttttgaattt
     2821 aaatttgata cactaatacc ttgagtattt tctataacag taagtgtatt ggataagtga
     2881 ttttcattgg taaaaatttg gaagtgtttt ttagcccagt ttctaactaa gtttgccatg
     2941 tcttcatgtc tgttgaatct gttatctagg ccttcatcta atatattatc taattggaaa
     3001 tctaaagcga acatgtgaga aagagatggt gttgaagggt attggtagtt tttcttgatt
     3061 aagtattcat acattgctaa caaatcaaga tatactcctc taaaaggtac ttgcttagct
     3121 ctatcgattg ctttttgaga aaatgtgcaa atagacattc ctggtggtaa tcccagtgct
     3181 ttttgtactg atgttataca aatatctatt ccaatcttat caacctcgac ttttattcca
     3241 ccggctgaac ttacagtatc tacacagtag attacatctt catacttttt aacaacttct
     3301 ccaatttctt ctattggatt tcttattcca gttgaagttt cattgtgagt tacagttatt
     3361 aaatcgtatt ctccggtaga aagcacctta tcaaccattt ctggcgttgt tgcctctcct
     3421 aattcaactt taaatatatc cgctgggaca ttattagcca ctgccatttt gtaccatcta
     3481 tcaccaaagg atccacagga aaatactgct gcctttttgg atgtacagga acgaatggag
     3541 ccttccatta aaccagttcc ggatgacgtg gataaaagta tcgtgttgtt tgtatagaat
     3601 agtttttgca tcttttcact tatactcttt tggagcatag atgcatcttt tcctctatgt
     3661 cctatcattg gtgtagccat cttttgcaag acttcttctt taacatcaat aggaccagga
     3721 ataaataatt tcttactcat tataattccc ccctttttag tttgtaatat ggatagcgtt
     3781 tttactgtcc ttatttttaa taactataat atcaagctga attaactatg tcaacataaa
     3841 atttattttt tatatataat ttattttata tattttatga aaacattcat tttctacctt
     3901 ttcataagtc tgaattttct gaattattct ctcaaaaaaa atatactttt tatctatata
     3961 aattaatttt tttagtatgt aaatttgacg ctattacgat aaatcttttt agtataacta
     4021 atttattttt atcttgtatt tttattgtat ttttgaattt tattttttct aaatataaaa
     4081 tttatgtttt aaaatgtaaa atttttattt aatcaaagtt caatatattc cactttttct
     4141 ttcgattctt caaaaaacca ttcttcatta gaaaaatcac cttctgcaat ttgtccaata
     4201 ggcattttca tatactttcc agttgctgtc actgctatat ctccatttgg tagaattatt
     4261 tctcctgtac cttcaaataa ttttctgttt tctttagtta ttcttccaac aacttttata
     4321 gtagcatctg ttggtactgg tttcttatat ttcaactcta aagatactgt tactccccat
     4381 atttggtcat catttatact tacagctctt ccaatagtct catccaatat tgcagcagac
     4441 atacctccat gaactctccc aggataactt tgatgccaat cctttgtatt acaaattgat
     4501 acaagctctc cattttcaag ttcataaaaa tctgctttaa gtcctagctc attttgtgtt
     4561 ccacaaacta aacaagattt actactattt tgcttcttca aaactttata ctttgccaaa
     4621 aaatccatct cctaaccgac attagtagtc gcttaatatt aaataattct aaaatatatt
     4681 atataatatt aataaaatcc ctgcaaactt ctaataataa aaataggcta cttcaatatg
     4741 ataagtttaa tttaaagtat tattttccaa aaaataactt tatcaaaata gaaactcaaa
     4801 gtagatataa gcctcttttt cgttattaaa atagtaaaaa tatattttac ttaatataaa
     4861 aaagagtgtc tcattaacta ataaagttta ttttgagaca cccctcataa aacaaattat
     4921 actacaatag atttttacta attctacaag ttatatattc aaattatatt attcaaatta
     4981 aactataatt ctaaatttca tttactatta attcgcccat ttcccttgtt cctacaacat
     5041 ttcccacacc attatagata tcaatagttc tataaccttt attgagtaca ctttgtacag
     5101 catcttcaat acacttagct tcttcttcta agttaaaact atgtcttaac atcatgctta
     5161 cagatagaat agtcgcaatt ggatttacaa tatcttttcc agctatatca ggtgcacttc
     5221 catgtattgg ttcatacata ccaaaactat cttctctaag tgatgctgat ggtaacatac
     5281 ctatagaacc cgtaatcata gatgcttcat cagatattat atctccaaat atattattag
     5341 tcactattac atcaaattgg ctagggtctt ttacaacttg catagcagca ttatcaacat
     5401 acatatgtga cagttctaca tcttcaaact ctttagctaa gtcttccatt atacttctcc
     5461 aaagtcttga actctcaagc acatttgcct tatctacact tataagtttt ttatttctat
     5521 ttctggctat ttcaaaagct ctcttaccaa ttcttcttat ttcattttcg ttatatattt
     5581 ctacatcata agcattttcg ataccatcaa tgatttttct tccacgttct ccaaaataaa
     5641 tgccccctgt taattccctt acaacaacaa aatcaatgcc tttctcaact atatccgttc
     5701 taagtggtga agcttctttt atactttcat acattgtagc tggtcttaaa tttgcaaata
     5761 aacccaatga ctctcttaat tttaaaagac cagactctgg tcttttatct cccttacatt
     5821 tatcccactt tggtcctcca acagcaccaa gcaatacagc ttcgtttttt cgacaagttt
     5881 caacagtttt attaggtaga ggttctccag tttcatcaat cgcgcatcct ccagctaata
     5941 catattcaca atcgaatcta tgattaaatt tacaacaaat tttatttaga acctttatac
     6001 cttcatctat aatttccggt ccaacaccat caccttttat gactgctata ttacaattca
     6061 tacttcacta ccccctattt gcttttattg agttaattaa gccttcattt aatataatat
     6121 tttgcataaa ctcaggaaat ggttctgctt tatattcttt tccttttgtt atatttttta
     6181 tcacacctgt agaaaaatct acttctatat tatccccttc atctatgtta ttggcagctt
     6241 cttcacattc aagtatagga agccctatat ttatagagtt tctaaaaaat attctagcaa
     6301 aagtagaagc aatcacacag gatactccac tttcttttat tacaattggt gcatgttctc
     6361 ttgatgaacc acaaccaaag tttttagttg caactataat atctcctttt tttaccttag
     6421 aaataaattt atcatcaata tcctccatac aatgcgttgc tagttcttta taatcagcta
     6481 tatttaaata tctagctggt attattacat ctgtatctat attgtcaccg aatttaaaaa
     6541 cacttccatt ggcaatcata aattttacct cccttaaatt atttctgatg gttttgatat
     6601 ttttccagtt atagctgatg ctgctgctac agctgggctt gcaagataca cctctgaatc
     6661 aacatgcccc attcttccta cgaaattcct atttgttgtt gaaatggatt tttctcctgc
     6721 tgcaagtata cccatatgtc cccctagaca tggaccacaa gttggtgtag aaactgctgc
     6781 tcctgcctct ataaaagtgg taatataccc tttttctata gcttcaagat atactttttg
     6841 agttccagga aatatcataa ctctaactcc atctgctact tttttgcctt tcattatctc
     6901 agctactact tcaaggtctg atattcttcc atttgtacaa gagcctatta caacttggtc
     6961 tatctttata tccttatcta tctcatctac tgttctagta ttttcaggaa gatgaggaaa
     7021 ggctactgtt tcttttagtt cagctaaatc tatttcgtat acttcatcat agactgcatc
     7081 ttcatctgct tcatatatat ttacatcttt tgtcatagtt gaacacttat gtgaatttat
     7141 atattccata gtcttatcgt ctactggaaa aattccattt tttgctcctg cttctattgc
     7201 catattacaa atagtaaatc tatcatccat agataagtat tctactccat ctccacaaaa
     7261 ttccattgac ttgtaaagtg ctccatcaac acctatctcc cctattatat gaagtattat
     7321 atctttacca cttatccatt tatttggttt attcttcaaa acaaatttta ttgctccagg
     7381 aaccttaaac caacattttc ctgtagccat acctgctccc atatcagttg aacctacacc
     7441 agtagaaaat gctcctaatg ctccatatgt gcaagtatga gagtcagcac ctataattac
     7501 atctccagca gttactatac ctttttcagg aagaagaaca tgctctattc ccatatctcc
     7561 tacatcaaaa aaatttacaa ctccttttga ttttgcgaaa cttcttgtaa atttacattg
     7621 ttctgcactc tttatatctt tattaggtgt aaagtggtct aaaaccatca ctactttagt
     7681 cttatcataa acatcttcaa ttcctaattt ttcaaattct ctaatggcta ctggtgatgt
     7741 tatatcatta cctaaaacta catctaaatc tgcttcgatt aattgccctt tcttaacttc
     7801 atttaattta gcatgtttgg ctaatatttt ctgcgtcata gtcatcccca ttttttattt
     7861 cctcctcaat ataactttta tttattgcat tcataagtgc attaatagct gctgtattta
     7921 tgttttccga tatacttaca ccaaaataga cttcattatt gtgctctatt cctacataag
     7981 ctacccctct tgaatgtgac ccttcctcta atgcatgttc ccagtaatat ctaaatttat
     8041 aatctttcat gccacattgc tttagtgcat tattaaatga atctacaggt ccatttccta
     8101 taccaacaat cctttgttca tttcccatat aacttattct tgcagtcatc tcaatcatgt
     8161 tagtatcatt agaatcatca ttttcatagt ttaattcatc cattgatttt attttatact
     8221 tcttaacttt ataagggctc tcaacttcaa tatattcttt cttaaacaaa ttaaatattg
     8281 cttcactact aagttcttcc ccaattctat cagattccat tttgactaca tttccaaaat
     8341 cagaatgcat attcttaggt atcataaatc cataatctgt ttccattata taaacagctc
     8401 ctccttttcc agattggcta tttatcctta taatttcttt gtattctctt ccaatatcat
     8461 gagggtctat tggtaaatat ggaacttccc agtaatcatt atctctgttt ttcattgcta
     8521 tcatgccttt tctaatagca tcttgatgtg aacctgaaaa agcacaatgt acaagattac
     8581 ctgcatatgg atgtctatca tgtaccataa gttttgtaca gtcttcatat atacctatta
     8641 tcttatctat attgctaaaa tctagctctg ggtctattcc ttgtgtataa agattaagtc
     8701 ccacatttac tatatccata ttcccagttc tttcaccatt tccaaatagt gttccttcaa
     8761 gtctatcggc accagccaat aatccaagtt cacttgcagc cgtacaagtt cctctatcat
     8821 tgtgagtatg caaacttagt ataatagaat ccctacaact tatattttta caaaaccatt
     8881 ctatttggtc tgcatatacg tttggtgttg ccatttcaac tgttgatgga agatttatta
     8941 ttactttttt ttgaggagtt ggtttccaaa catctatcac tgcctcacat atttcaagtg
     9001 cataatccat ctctgttcct gtaaaactct ctggtgaata ttcaaaagta aattctgttt
     9061 ctggatattt ttttacttct tcattaaata attttgctcc ttcaactgct atatctatta
     9121 tttcttgttt actcatatta aaaaccacat ctctttgaag aacagatgta gagttatata
     9181 aatgtataat tgctttttta catcccctta ttgattcaaa tgttttttct ataagatgag
     9241 ccctagattg tgtaagtact tgtatagtta catcatctgg aatcatattc tcatctatta
     9301 attttcttaa aaatgtatac tctgtatttg atgctgatgg aaatcctacc tctatttctt
     9361 taaatcctgt atcaataagc attttgaaca ttctgacttt ttcatttaca ctcataggtg
     9421 ttggcagtga ctgatttccg tctcttaaat cgacactaca ccaaattgga gccttaaata
     9481 tttcattatc tggccattta cgatttacta tttgcatttt atcatatttt ttatactttc
     9541 cacacttcat gttatttccc cctttttttg ttttttgtat tcagtatacc tcatatcgac
     9601 gcaatcctgc acaattcata aggataaatg tcaattttat cattagaata aaaaaaacct
     9661 catctctaat aaaattagag acgaggtttt gctcgcggta ccactctatt tagctttcta
     9721 aaaagctccc ttagtaatag ccaatacaat aattataatg actatatttc ctataacgtg
     9781 gaattactcc gattattcta agatttaaat cttagaaaac agctcctaga caagttcaga
     9841 actatcaact actaaatcac accatccttt agctctctga aagtttcatt gttcttacta
     9901 cttctattca tcactttttt aatcatttaa attttctaaa tattttgatt taatagttat
     9961 tatatacaca ttaaaatgta ttgtcaacac aaaaaataat tatctcaatt ttttattttt
    10021 gataagcaat tctataaaac atgctaacta ttgaaaacag tatgatatta tgccttaaac
    10081 tttttcatct tttcatctat gatattcaat aatttttttt catcttccaa actcaatgcc
    10141 cttaatctaa ttttataatt agattcgtca gtgtatatat ttaatatatt ttttactact
    10201 tctatttttt ctattatctt ataattaaac ttttgacttt gtttagattt tatactgaat
    10261 atacattgat tatttattaa tattgaatga ttatcgagag taatatatac ttttccctca
    10321 tatttatttg aaaaatcacg accttgttta aatagaaaaa ctgctgttat tatacataaa
    10381 atatataata ttgtcgttga atatataaaa attgtagcta aaattataag tgctaaaacc
    10441 atatatatag tcattccttt tttaaaataa ccattgtcat aataattcat atagataaac
    10501 ttatttttac tcaaattttt tcactcccaa gattctaaaa atttatatca aaaaattcta
    10561 taatttcatc ttcatgataa tttatgacta attcatctgg aaaatctact gcttctaaaa
    10621 ctttttctgc attttcaaat tctcctatag aataacatat atgagagtca gtaccaagaa
    10681 ttactcttgc accatatgtt ttacacaatg tcaacatatg acttacattt tcccaagtac
    10741 ccgttctatg agagtttgaa cttaatgatg aattatttat ttctaaaagg atatttttat
    10801 ctttagcttt ttttactata ggttcataat ctaaaggata cctactgtca tctggatgtc
    10861 caattatttt taccttaggt ttatccataa cttttaaaat agcatttgta tttttttctt
    10921 ttgtaccaga ggctatacaa ggtctatgca aacttgcgat tatataatcc aaatgtttca
    10981 acatatcttc ttgaacatct aaattaccat tataatccat tatatttcct tctattcctc
    11041 taagaatctt tacgccttgc acttcacgtg gaagcaaatg caaattataa aaatagaatg
    11101 gatgtggtcc tcctggcata ttaggtccat ggtctgatat tcctaagtat tttatattct
    11161 tcttactcgc ctcttcgaca ttttccttga aagtactatg tgcatgtcca cttattagag
    11221 tgtgacaatg tacatccata atagactttt tcattaaatt tcctcctaat attttacata
    11281 aattcgtgaa aatagctaaa gggctatttc aaaactaaag ctgtcataat agacagtgta
    11341 acgttttaaa acagccccat tttcaattat tttacacttc taaatcttta aatttatctt
    11401 taaataattc tccaatactt cctaaagatt cctctacttc taggtattct gttatatcct
    11461 cttctggctc tctagttgct tcttttattg aaaccaacat tcttttagtc tttggtttaa
    11521 aatcaagaat cttaacttta acctcatctc caatattaac aacattagta actttagcca
    11581 ctctagtatc actcaattca gatattggta aatacgcatc tacaccttct ttaatttgaa
    11641 caaaagcccc tttttctatt attctaagaa ctttagcatt gacaacatca tcaatttgta
    11701 cttcttttgc tattaaatcc caagggtcat tatttatgtc ttttaatgcc aaggcaattt
    11761 ttttagtctc tttatttaca tccaacacat aaacttctac ttcttgtccc tctgaaacaa
    11821 catcttcaac actttcaact cttgtccaag ctaaattatt tatatgtgct aatccttcaa
    11881 tcccaccaat atccacaaat gcaccataag gcattatctt tgtcacctta ccatctcttt
    11941 tttgtccaac ttcaagagaa ttaaataagt cttctttagc tgctttgata cgagcttttt
    12001 cttcttctct ttcttgctta attcttgctc tttcagcttc tttttctgcc ttaatttgag
    12061 ctcttctttc ttctctctct ttatttatac gctcttgtaa tacatctcta tgagatgcta
    12121 caagtctatt ttttctaaca tccaattctt ttatataaac ttctaagtta agaccaacaa
    12181 atttagatgt atctgtaata aattttgtat caagttgaga aattggtata aaaaatgtat
    12241 atgtattgta attagcaaat acacctttat ctatattctt ttcaacattt acagttatta
    12301 ttctatgttc atcataagcc ttttgtaact tggcaaaatc atttcttttg tcaagttgta
    12361 atttagataa agttattgta ccatcttttt gagatacttt tgttattaca gcagttattt
    12421 catcacctat gtggtatata tcttctatat attgattttt ttctatgttc aattcactta
    12481 taggtattat tccatcaaac ccatagtcta atcctaattg tacttcatca tttctcactg
    12541 cagttatttt tccagttgtc aattctccaa ctttcacttt gctgaatact tgttcttgct
    12601 ggtcaagtaa ttcttgcatt gttaaatcat tttccattat aaacatcctt tcctcttatt
    12661 gattatcata tctgtaaata ttataccatt aatattgcaa atacttacat ttttcttact
    12721 ataattttta aaataaattc aacttcatat atttatacat tatagaaaat atcttattaa
    12781 attaaaatac aaattctaat ttttatctaa taaaatattt tcataaaaaa tttatattgt
    12841 atatcttatt cttttattgc tttaggtttt gatagaaaac atttataaaa tataaagaaa
    12901 gaaattgctg ttatagtagc agctgttata tctgcaactg gctctgctaa aattactgca
    12961 aaaagttttt cattaaagaa tgttgggagt ataaatatta aaggtattaa aagtacaatc
    13021 tttctaagta gagctaaaac caaagatatt gttgcttttc caagtgctaa aaatgtctgt
    13081 tgacaagcta tttgaatccc aaacataaaa gcaccagcaa aatatatttt tatactccaa
    13141 ctagtcatac taagtaactg cggattctta ttaaatatac taacaaaaaa tgcaggaaat
    13201 atcattaata aaagccataa aatagtagaa tatgtaaaac atgacaacaa acacaactta
    13261 aatgtctttt ttactctatc aagttgacct gaaccataat tataacttat tattggttgt
    13321 gctccttgtg taacccccat attaggaagc ataagaattt gcattatact gctcattatg
    13381 gccattgaac ctactgctaa atctcctcca tacatttgta atttactatt taaggctata
    13441 agtactaaac tttctgtaga ttgcattata aatggagaga ttcccaaacc aagcactggt
    13501 aagatgatac ttgcttttgg tatcatatac ttctttttta ttttaagtat acttttcttt
    13561 ccaaagagaa acataagtac ccaaatagca gaaacgcctt gtgcaattat agtagctaat
    13621 gcagctcctt ttacacccat attaaagcca aatataaaaa tcgggtcaag tattatgttt
    13681 attaaagcac ctatagcaac tgtcatcatt ccagttgtag caaacccttg ggtatttata
    13741 aaagtattca tacccattga aatctgaaca aacactgtac ctattaaata aatatttaaa
    13801 tagtccattg aatatccaat tgtagattca cttgctccaa acatccaaag taaaggctct
    13861 tttccaatct taaaacctat agtcaaaagt attgctaaaa tcaccagtac agaaaaactg
    13921 ttactcatta ttttctctgc ctcatcattg ttttgctcac ccatttttat agcagctaat
    13981 ggacctcctc ccataccgat taaataacta catgcagtta ttataattat tattggaaat
    14041 gccactccaa caccagccat agcaacttct ccattttcca ttctaccaat aaaaatccta
    14101 tcgactatgt tgtataaaac atttacaatt tgtgcgataa tagcaggtgt agcaagttta
    14161 aataataatt tacccacact ttcactacct aagttagtgt ttgaattttc cattttaaaa
    14221 cctcctcaat ccaataatta aaatgtatca ctaaaatttt attctatata aaaatttcat
    14281 tttattagca gacaaattta gagtactttc cctacaagta atactatact atatttcttt
    14341 tgccatagta aacattttag aactaactga agttaaatac tttatctcat tttttattta
    14401 ttattaaaaa tttttcacac atactcataa aaaaactatc caagtatatt acttagatag
    14461 ttttatatta tatgtaaaga aagagctgaa actataaccc ctgataacaa attaccaaga
    14521 aatactccca taaaagcatc ttttattctc atttttaata tggaagctat agcagatgca
    14581 gtccatgttc ctgtagttgg cagtggtata cctacaaata gtattatacc aattatcgaa
    14641 acacttttta atttcttcat tcttgagttt atttttctat caattacatc agcaatccct
    14701 ttaaatagtt tcttccctct taaaaattgt aatacatgtc tgaatgtcaa tatcaaggga
    14761 atcgatacca atgtagaacc tattacacta aatacataaa cccatatagg atttaagccc
    14821 attgcaatac ctataggaat ggctcccctc aattctgtta caggaaccat agataaaatt
    14881 aatatgtgta aatatcccaa actattccct cctattctaa gtgacattaa gttatgaatt
    14941 tatttgctct ataacctcac catccttttt actaactaaa aatgcatttt tagtcaatga
    15001 aagcattggt aaatcactct tagagtctga atatgcatat gaattttcat aatcaatctc
    15061 tatcccaagt ttacctaata cagcctttat tctttttaat ttttcaattc ccttacaatt
    15121 ttctccaatg aattgatttt tatatcttga attttcataa aaaaactccg tgcctataac
    15181 ctcgtctgca agaccataat acttaaaata tttcatatat gcatatggag atgcagttat
    15241 cataattata acattatctt cttctttatt tttgtaaata acctttttca tagattcgtt
    15301 aatttttttc attatgcaat catcaaaaaa cttttttaaa tcttcttcac taaaatctac
    15361 tattggataa aagattgcct cttttgcctt cttcaaaggt atcattctaa ttaaatacat
    15421 aaatagaata aaaataatgt atggcaactt tataatagtt ttaaaggttt tattttttaa
    15481 agagtaaata aaaaaatcta tcatgctatc agtatttata acagtataat ctacatcaag
    15541 taataataca ctttttttca taagtatttc cccatcctat cactaaaaat atcactaaaa
    15601 aatcaatatt attaaacatg taattatcca taagattacg tttattttta aagcattatc
    15661 ttttaggaca atatcagtag gattattttc atttgtagtg ttaattatat aattatatct
    15721 aagtacacca taaactacaa atattgttgt aaatatcata tgtggctttt ctggattact
    15781 tgtagaatac aatgcataac atacaattgt agaacttaat ataactatca tcatttgatt
    15841 tagattttct atgtcataat cttccaatat ttttctatgt tcaactgcat catctcttag
    15901 agtttctatt tcttttttac gttttccaag agctaagtac agagataaga aaaatgtaca
    15961 taaaattatc caatttgata aactaacact tatggctaca catcctgcat aaaccctaag
    16021 tataaaacca agagatattg aaaacacatc taatagtatt atattcttta gtttaagaga
    16081 atataatata ttgtttatca aatagagact gagaattata gaaagattaa aactaacaac
    16141 tgttgataaa cagactgaaa ttaaagctaa tactacaaat agtatattag cacttttttt
    16201 gcttattctt ccacttgcca ctggtctatt acatttttta ggatgttttt tatctttatc
    16261 catgtctata gcatcattca gtgcatatac tgatgatgac attaggcaaa aacacacaaa
    16321 cgcaataaac gtatttaaaa ataaatttat atttagtatg ttgtttgaaa aaattatagc
    16381 tgaaaataca aagaaattct ttatatattg ctttattctc attaacttta tataatctga
    16441 aattttagta agaagcctta tacttgtatt ggtctgtttc ataaacttac taccctttca
    16501 atattatttt ttaaacagga tatgatttta tccctatgtt taaaatttct ataaatccat
    16561 tgacattact gattactata gtaaccaata acagcgatag aaatatatta tacatattct
    16621 tttcattttt tctaagcgct ttcgtaaagt aagccaataa aattataact gcaaaactaa
    16681 aatttatagc cataatactt gaattgacta gattatatcc tactatacca tgtaaagtta
    16741 gattgaatat aattattccc atacatagct taggaactct tttttctgaa ttttcaataa
    16801 ctgataaaac acaaaaacat aaagctaata atgtaagaat tactggtatt gtagcaactt
    16861 ctccattttg caaaaataac ccttttttaa tgcttgctcc aggaaaataa aaaaccgaat
    16921 acaataatct ctctataatc attgcaatct tttccaaaat tggttctcca cttataaatt
    16981 tcgtatcata ctgtattaca tttccaaggt tcataaaact ctcataagac ttcgttgaaa
    17041 ctacacaaat atatgccatt aggataaatg taattaaatg ttttaacatt tttcctattt
    17101 ttaatttaaa ctcaaatatc atattaaaaa aatacaaata tatattacta atagttattc
    17161 caaatagagg aatagctgct aattctacta aataagaatt ttttcctttc atcttatttg
    17221 caataactat gaaaaatatt aatgaaaatt ggctatatat aaatctctct acatttaaag
    17281 tcataaatag ctgtggaaaa gaaaatatca ttatcatagt aagtaaaatt aaagttaatc
    17341 tctgcatttt tatcttttta atatgattaa agactaaagt tattgacata gcactagtta
    17401 ttatttgtag acaaattagt ccaaacccat aggcactatt aaaatctgtt gtcccaggat
    17461 ttgctattga agtacaaaat aggtatattg gatatgcaaa aattgatata attgctgaaa
    17521 acaatatgtg cttactattg tcttgactta tagcaaatac atttaaattg aataaaactc
    17581 ctgtatctgt atctaacaat acatcataag tattgtttaa atcataaaat atagtagtct
    17641 tagaaaaaaa tatcttagtt attattaagt aagttattac aactaatata aacaaaaaca
    17701 tctcgttttt atattttaat attttactca tcaatatatc accatccatt cattatatag
    17761 gatttgctga cacctacaat ttattcctta tatttaatag ccatcttcat aatgttctta
    17821 cttgttttat tgtaattgaa taactcttta tttacaacca caatatttac tttattgatg
    17881 ttataatcag tcaagtattg tttatataac atagattagt gttgtataaa atgagaattt
    17941 gtaagcaata ttaatgtaaa atatttgaaa aactttatat aaaaatatac aatgttgctt
    18001 atggaattaa actataacaa attaaagcat aatttaagga gacaaaatta taatctgtct
    18061 ccttaaatat taaaactata ttttaaaaca ttactaaata ctatacaaat ttcaaatata
    18121 gtataaaatt aattagactt taacactatt agctccatct tcttcccatt ttttaatttc
    18181 ttttaatcta tctattatag gtaaatgctg agtacattca acttcacatt ttccacaaga
    18241 tatacattct ttagcttttg ccttactatt cattgtgtaa ttccaatgtc ctaagccata
    18301 aactaacttt gtcatttctt catcactctt tttaaacatc tgcttttcat tataaaaaag
    18361 catataggct ggggtattaa taccttttgg acatacttta caataaccac aacctgtaca
    18421 tatttcgttc atattcttat ttaatctagt ctcaatatca tgtatatcct catctgaata
    18481 aattctattt tcgtctgcaa ttttacatgc atcatctata tcttgttttt tattaaaccc
    18541 tatcaaagat atacttattt gtgaaattcc tatgttgaat cttaaagctg attctatagt
    18601 actttcgcca tcttttgata gaaaagaaag ttctttatta tactttggaa tagttccacc
    18661 acttaatgga ttcattgcca caacaccata tccattatca acagcatatt tggcaccttt
    18721 tagcctatat ggaaagttta atatatttat tcccatagtt acaccttcaa acttattttc
    18781 acttagaata tctataacct catctcctgg ttgatgagat gaaaatacta tatggtctat
    18841 caatccttct tctttacatt tcaataatcc ttcatattga cctccaggtc tcattgctag
    18901 ttcgtaatgt tccatctttc taatattcca aacatgataa aaatttattt tagatacgcc
    18961 taatcgttct attgattttt ttacagcctc tatagctttt tctgatgtat cacatgcttt
    19021 tggcttttct ttagttgata cataaaaatc ggtcttacct tcctttatca tttgtctaaa
    19081 ggctagtcca aatatatctt cacttctgtc atcacaatac cctggtgctg tatcaaagta
    19141 attaatacct ttttcatacg catatttgac taaatcagca ttttcttgat tacttttttc
    19201 taaatcaaac cttaatcctc catagccaac tactgaaacc attttccctg tctttccata
    19261 ttcaacgtat ctcattgtaa attcctcctt agatatatta agacttaatt tctttataga
    19321 aaatattata ccataaataa tttttataaa ttatttatgg tataatattt tttttataaa
    19381 ttatattttt ttataacttg tattttataa ctaagttaaa aactataaaa aggttcaata
    19441 tggactatta cttgagcatt ttcattaatt tcttcttgta tttgtttctc aattttatgt
    19501 gttaaatcat gagattgctc tacgcttata aatggatcta ccattacatg catgtcaata
    19561 taaatagaac ttttgctacc tctacttctg atattgtgaa catcccttac ctcattaaat
    19621 tcaaacacaa tttcttttat ataatcctca tctacagcca ctctatcgac caaaattcca
    19681 atagatggcc taaatattcc ataagccgaa taaattatga atgcagaaat aataatagga
    19741 actatagatt ctattattac tggaaatcct aacttaacac caactaaagt aaccaataca
    19801 cctagagaca caaatatatc acttcttgta tgcaatgaat ccgaaatcaa aacataacta
    19861 tttagtttag tcccaattct atattcatac atgcatacaa aaatatttat aaaaagtgta
    19921 ataattaaag caattaaact ttctatagtt atagcaggta caactggatt agctattctt
    19981 aacactgcac taagaattat cttaattgct ataacaaaca acataccact tataaataaa
    20041 ctggttataa tttcaaactt tttatgtcca taaggatgat ttttatcttt tggtttagat
    20101 gcaaagaaaa tacctataag ccccactata ttagatgctc catctgacaa agaatgaaat
    20161 ccgtctgctg tcatactata gctttttatt tgattaccaa taatgatttt taataatgct
    20221 actgcaaaat tagcaaataa aattatccac aatacttgct tcactttctt atagttatcc
    20281 ataacaaatt ccccctccct tcgattcaat aaagaaagct atctatcaat atcaatcaac
    20341 cagacaataa aatcgtatat aagcacattt actatattta tattcaaaat tggttgtact
    20401 tgttcattct atttgaaact taattataaa taaaaatcgt aggtatggtt ttggtataca
    20461 taaaaataag agactgtctc aaaataaaga taatcttctt ttttattgtt gaaatgataa
    20521 aatatatttg atttcatata aaaaagagtg cctcatgaac taaaaagttc attttgagac
    20581 actcccttaa atttttgata taaactatta attttttata ctaagccttc tttaactaag
    20641 aatgaatgag caacttcttc tggtgattta ccaagttcat ccacttgata gttaagttct
    20701 ctcatagttt cttcatttat ttttcctgaa agcatattca taactttttc taactcagga
    20761 tatttcttca aagtatcttc tcttactaat ggtgctgcat aatatggtac aaagaaattt
    20821 ttatcgtcct ctaatatctt aagattaaac tttttaataa gaccatcagt taaaaatgca
    20881 tctataacct gagattcatt attatctaaa gctgtatatc ttaaacttcc attcattgct
    20941 tttacatctt tgaattttaa accataatct ctagaaagcc ctacaagacc atcttctcta
    21001 ttttcaaact ccaaagttgg tgagaatgta aattctttac catatttagt caaatcagaa
    21061 atagtattta ttccatactt ttcagcagtt tcaggcatca tagctaatgt gtaagtatta
    21121 ttaaatccaa gagggtcaag taaagttaac tgattttctt tttccatagt atcttttaca
    21181 acattatatg cttcatcagc atctttaatc ggagcatgtt tcattacatt taccaaaagt
    21241 gtacctgtat agtcaacata catgtctaac tcaccagatt taattgcatt aaacgcaaca
    21301 gatgaaccac ctagatttaa attcttctta actttaagat tcgtattttt ttctactaat
    21361 tcagcataaa cattaccaag tataagttgt tctgtataat ctttagaacc aatagttatt
    21421 gtatcttttt tacttgaaaa actagaacta aaaacaacga acaacattga aacaacaacc
    21481 actacagata ttactttaag tgctatataa ttcttcttag gtgcttttgg atttaatcct
    21541 tttggagtaa ctgcaacttc aattttacta aataaataat caactaacag tgcaattata
    21601 catgatggga ttgcccctgc taaaatcata ttattattta cagtttgaac ccctgaaaat
    21661 actaaatatc ccaaaccacc agcacctata aatgctgcaa gagtcatcaa accaacagct
    21721 gtaactgcag atattctaac tccacccatt attataggaa gcgctaaagg aaattgtatt
    21781 ttaaataatg tctgatttct tgtaagacca atacctttag ctgattctaa tactactggg
    21841 tcaatacttg ttatacctat atacgtattt tttacaattg gtagtaatga ataaaccact
    21901 accataaata tggctggttt acttcctata ccaagtatag gtacaagaag acccaaaagt
    21961 gccatagacg gaactgcttg gactaagttt acaaatccaa gtacaaactt tctaagagat
    22021 gagattcttg aaataattat tccaagagga acaccaacta ttatagctat taaaatagat
    22081 gtaacagtta gactcatatg ttgtattaaa agctctataa tcttatcttt ttgtaatagt
    22141 ataaagttaa aaaaattagc caagaacact cacctccatt tctaagaatt gttcacttaa
    22201 aacagaaagt aaactacttc tagtaataag ccctaccagt ttattttcat cagaaataac
    22261 cggaatatat ccaaccgaat tacgattcat tacatttagt atttctacta aattatcacc
    22321 ttcatttaca tgcaaaggct cacttgacat aatatcagca agtaatattg acttatcatc
    22381 aatatctttc atatctttta cagtaacaat accttttaat gtttttgctc tatctataat
    22441 tagtaagcta tcaaccttac tcgttctcat aatttctatt ccttgagtta cagttctagt
    22501 agaattaaca gcaataggat tttttatcat aatatctctt gcttttatat attctggatt
    22561 atcccaaact ctatcttctc cgataaaatc ttttacaaag tcatttgcag gctttctaag
    22621 tatattttcg ggagtatcat attgtgctat tcttccatcc ttcataatac aaattttatc
    22681 agctatcttt aaagcctcat ccatatcatg agttacaaat attatagttt ttttcaattc
    22741 atcttgaaga gaaaataatt gttcttgtaa agatgttctc gttattgggt ctaaagcact
    22801 aaaaggctca tccatcaata ttatttcagc atcagtagca atggctcttg caacaccgat
    22861 tctttgttgc tgtccaccac taagttctga aggatactta ttcaaaaatt catcactatc
    22921 aagtcctacc atctcaagta gttgcaacgt tcttttttca atttcttcaa cggatttttc
    22981 cttttttagg cgtggaatta gctctatatt ttcttttatt gtaagatgag ggaataggcc
    23041 tgtgttctgt ataacatatc ctatttttct tctaagctct atttcatttt cctgagaaat
    23101 tggtttgcca ttaatatata tctcaccaga tgtaggtttt attaatttgt ttataagctt
    23161 caaagtagta gtctttccac aaccacttga accaatcaaa actaccaatg tacctgtttc
    23221 tacaactaga gaaatatcat ctaatataac attatttcct attttttttg ttacatttct
    23281 tatctctatc ataatttatc atcccctttt tcgaacaagc aaccttcttc aatattataa
    23341 gttgttttta taattatatc atactatttt atttatgtaa acaaagtacg atttttaggt
    23401 cactatttgt ctgaatattg cta
//
LOCUS       DAELLV010000034        23331 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.034, whole genome shotgun sequence.
ACCESSION   DAELLV010000034 DAELLV010000000
VERSION     DAELLV010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23331
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.034"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..891)
                     /locus_tag="KPD58_001148"
     CDS             complement(<1..891)
                     /locus_tag="KPD58_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HBG0264931.1"
                     /translation="MYILNLNTRETIEDFRDKFYVAENSYLILSAPKNLKLLKETLDI
                     DEITFNDCLKFDEITKLDLFDNYDFLSLNTFELRDGEAVIEEVNMYLSDNFILVVVNE
                     EHFLFEFVKNIILKNSQLEKNPVINLFKINYLILREVIKNGFESLEKVEELILQIEDE
                     MMDNINKNHVSRISDVRGLTRIIVKNTRPLLYIGDRIVKENIRYLKYSNVKKYNLENF
                     QGIDFGIDKLYNFALSTRELADKLLDIYSSRVGEKTNNLITKLTLLTAISAPLTIITG
                     IYGMNFRYMPELNWIYGYPAT"
     gene            complement(1248..1946)
                     /locus_tag="KPD58_001149"
     CDS             complement(1248..1946)
                     /locus_tag="KPD58_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0264932.1"
                     /translation="MSKKLILLVEDDKTIRKFISTALLTQDYDVKEAITGKEGISIAV
                     SYSPDVVLLDLGLEDMDGIEVIKAIRQFSNIPIIVVSAREQDRDKVEVFDAGADDYLT
                     KPFSIVELLARVRVAFRHSQVEAQQKDDVKSTFEVDKLLIDFDKRKVIVDDVEVHLTP
                     IEYNILSLLAKHHGKVLTHNFIIKEIWGSVIGNETKSLRVFMATLRRKIEKQPANPRY
                     IITEVGVGYRLNDE"
     gene            complement(1980..4682)
                     /locus_tag="KPD58_001150"
     CDS             complement(1980..4682)
                     /locus_tag="KPD58_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HBG0264933.1"
                     /translation="MDRPNPDILLEKIKNEEEKLSRGQLKIFFGYAAGVGKTYSMLES
                     AQNLKKVGVDVVVGYIEPHTRPETLALLDGLEILPVKDIEYKSIKLKEFDLDAALARK
                     PEVILVDEFAHSNVAGLRHTKRWQDIEELLLAGINVYTTVNVQHLESLNDIVEIITNV
                     SVKETIPDKFLDTNTQLELIDVEPDVLLERFNEGKIYKKEQATRAKNNFFIKDNLVAL
                     REIALRKTAERVNKEVQMTRLSKGDVTVIPTSDTLLACISPSPSSAKVIRTASRISDS
                     TFAKWIALYVETPNTARLPFDEQKQLQNNLKLAKKLGGEIIVLHGENIIEQILRIAKL
                     RNVTKIVIGRNHSSNKRFSKRFKKDIVDKLIDEVDYIDIHIIPYKFASDVKYKPKKDK
                     SSIKSKFKISKVDFIKLIFITLMISILAYAVQSMGFIRENILLIYMLGVVLVSMWTKG
                     YSTGIISSVFNIVLLNYFFTAPLYTLSIADSNYIVTLVVFSIVGIITSTLTSKIQHEA
                     ETAAKREENTKMIYQIIKGFLRLSNKDDIVNKGIELLSLSLSRDVICYLNVDKHNKSK
                     LYKKNTNNKDKNDLDSEDEKAVASWVLSNSTVAGNDTDTLPGSKGYYIPIVGMNLTLG
                     VVGISCIDSKLDTEDISLIETIIAQMAIALDREILSEAKENTNLEIERERLRSNLLRA
                     VSHDLRTPLAGISGAVSTIIKNKGTIGQDIIDELLSGVYEDTQWLIRLVENLLSMTKI
                     DEGKLEVKKHSELVEEIISEALQKIKKRIENALIDINIPEQILFVPMDAKLIEQVLIN
                     LIDNSLKYSKEDCKIEINVYEKDDYVWFEVSDNGPGISKELKKHIFDRFFTGEEGAKD
                     SRKGVGLGLSICKSIIQAHKGEIMVENNKDKGSTFKFSLPKENE"
     gene            complement(4860..6677)
                     /gene="ftsH"
                     /locus_tag="KPD58_001151"
     CDS             complement(4860..6677)
                     /gene="ftsH"
                     /locus_tag="KPD58_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBG0264934.1"
                     /translation="MDEHKPQKRSALYYYTVIIIVVMFLNALIFPSLMDKKMVEIDYG
                     KFLTMLDTGKVKSVEIENNRIAINPSSSKDKNIYITGRMDDPELVDRLKNAKVEFTKV
                     IPKENSPLLSILLTWIIPIGILMLFGNLMMKSMQKRMGGNAMQFGKSNAKMYVSAQSG
                     KRFNDVAGQDEAKEALTEIVDFLHNPEKYKKIGAQMPKGALLVGPPGTGKTLLAKAVA
                     GEANVPFFSISGSEFVEMFVGMGASRIRDLFKQAKEKAPCIVFIDEIDTIGKKRDNGS
                     GLGGNDEREQTLNQLLSEMDGFDGGIGVVILAATNRPDSLDKALLRPGRFDRRIPVEL
                     PDLGGREAILKVHAQNVNVSSDIDYNAIARSTSGASGAELANIVNEAALLAVKSGRDI
                     VEQQDFEESVETVIAGYQRKGAVISDKEKKIIAYHEIGHALVAAMQKHSAPVHKITII
                     PRTSGALGYTMQVAEDESVLMSKEEAIDKITTFTGGRAAEEVIFNTCTSGASNDIEQA
                     TKIARAMVTRLGMSEEFDMVALETVSNQYLGGDTSLACSSDTAAKIDEEVFKIVKACH
                     KNAIEILENNIDKLHELANYLLEKETITGDEFMEILNLN"
     gene            complement(6708..7151)
                     /locus_tag="KPD58_001152"
     CDS             complement(6708..7151)
                     /locus_tag="KPD58_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0264935.1"
                     /translation="MDEMSFKKELLELTRDINMKFTTLLSDFYQPLGITAVQALILSE
                     LCEHGEKKISDLGKNLNMTNSNVSVICQRLEKNGFLNRIRDIEDQRIVKVKVTNKSLD
                     IQEHISSSIFDSYFENMTSEKLQDMEDIIEGLEKLNKLLTYIDCK"
     gene            complement(7642..8532)
                     /gene="metA"
                     /locus_tag="KPD58_001153"
     CDS             complement(7642..8532)
                     /gene="metA"
                     /locus_tag="KPD58_001153"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HBG0264936.1"
                     /translation="MALILPKGLPVINKLLDEGIDVIYKEDFKKELKYEENIDTKIAI
                     LNLMPIKIDTELDLLRRIDKTGFNVSVEFIKISTRESKRSCNEYVKEFYKTFDEAKGE
                     YFDGFIITGAPVEQMEFEEVDYWNELEEIMDYSKRKTKSTLYICWAAQASLYKYYNVK
                     KLPLSQKCFGVFKHKVDKDSKIVDGFENEFFAPHSRHTTVNIEALKENKELSIVSHSK
                     EAGPYIITNSRDVFVMGHSEYDKYTLDKEYKRDINKGDKISIPQNYYINDDPSEEPTV
                     KWKKHSELLFRNWIKNYLIQ"
     gene            complement(8562..9827)
                     /locus_tag="KPD58_001154"
     CDS             complement(8562..9827)
                     /locus_tag="KPD58_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HBG0264937.1"
                     /translation="MYNKETICVQGNYKPGNGEPRVLPLYQSTTFKYSSIDQLAELFD
                     LKVDGHIYSRISNPTIQAFEEKISLLEGGVSSVAVSSGQSANMLAVLNICKSGDSILC
                     SSKVYGGTFNLLGPSLKKFGIDLISFDLDSSEDEIVELAKENTKVVFAETLANPTLEV
                     IDFEKIANVAKRINVPFIVDNSLASPVLCNPLKYGANIVTHSTTKYLDGHASSVGGII
                     VDGGNFNWDNGKFPELVEPDPTYHGISYTQKFGNAAYATKARVQLLRDYGNCLSPFNA
                     YLTNLNVETLHLRMERHSENALKIARFLEKHENVDWINYPGLEDNKYYENAKKYLSRG
                     CSGVLSFGVRGGLENAKKFVEKLQIASLVTHVSDVRTCVIHPASTTHRQLTEEQLIAS
                     GVLPSLIRLSVGIENVEDLIADLNQALNF"
     regulatory      complement(9937..10039)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(10093..12750)
                     /locus_tag="KPD58_001155"
     CDS             complement(10093..12750)
                     /locus_tag="KPD58_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBG0264938.1"
                     /translation="MENHKQMPWHSRATHEILESLDSSEEGLTDTEAEKRLKENGLNE
                     LRKKPPRTTLQMLWSQITDAMVMILIGAAILSLIFGEFTEAFVILIIVTVNAVIGIVQ
                     EKKAESSLEALKNMNSPTARVMRDGEENVIPASNLVVGDIVFLEDGAMVPADLRLIET
                     SSLKIQEASLTGESVPSEKDADTILPKECVLGDRSNMAYTSSIVTYGRGVGVVVATGM
                     DTEVGNIAHLLDSQDDFDTPIKRKLNTVGKTLSVIGIIVCVVIFAIGAFYQRPLLPMF
                     MIAISLAISIIPEGLPATATIVMALGVQRMAKRNALIRKLPAVETLGGATVICCDKTG
                     TLTLNKMTVTHIAVNGDFESGKVTSIDTASEKHPLVYKEIVYAGALCNDACINPDKTD
                     EILGDPTEGALIFMAKKFGIDQEALEEEYPRVFEQPFDSDRKCMTTVHNIDEKIIAYT
                     KGAVDEILDLCTKILTSKGERNITEVDKKNIHELCLNMSKDALRVLGFAKREISSIPK
                     EDSENIEYDLTFIGMVGMIDPPRTEVIDAVSTCKEAGIRTIMITGDHKVTATTIAHEL
                     GIWSEENTVISGDELDNLSDDELDEAVKNTTVFARVSPFDKLRIIQSLKRIGEVPAMT
                     GDGVNDSPALKSADIGIAMGISGTDVAKDSSDMILMDDSFTTIAYAIKEGRRVYRNIQ
                     KVIQFLLVGNIAEILTLFVAILLNWDTPLLAVHILWVNLATATLPALALGVDPPSRNI
                     MKHQPVKSTTLFEKNLIQRVVTQGVFVAIMTILAYKIGLNIENHSVGQTMAFCVLAFS
                     QMLRAFSQRSNTDSMFNRNNGKNPFLLISFLTSAFLIAVILFVPVFRNAFNLTVLNNL
                     EWLITIVLAIMSVIQVEVGKIIKRKKLKK"
     gene            complement(12878..13621)
                     /gene="yaaA"
                     /locus_tag="KPD58_001156"
     CDS             complement(12878..13621)
                     /gene="yaaA"
                     /locus_tag="KPD58_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HBG0264939.1"
                     /translation="MIIMLSPAKNMKNIEVFDRDLSLPCFIDNTKEIVENIKTFGIED
                     FKNKMKINEKLAVLNKNRFESIKFDRLGNPAILTYDGIQYKNIEAENFTRKDEEFANS
                     CIRIISGLYGVVKPYDSIYEYRLEMQTKLQVGEFKNLYEYWGNRIYKELIKEKTAIVN
                     LSSNEYSKSIEKFIKDSDTYITCTFKVNKNGILKVESTQAKKARGMMTKYIVKNRIRD
                     IEELKKFDLEGYKYKENLSNNSEYIFVKE"
     gene            complement(13631..14095)
                     /gene="bcp"
                     /locus_tag="KPD58_001157"
     CDS             complement(13631..14095)
                     /gene="bcp"
                     /locus_tag="KPD58_001157"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HBG0264940.1"
                     /translation="MLSIGTKAPEFTLEDKDGNKVSMSDFKGKKVVVYFYPKDNTPGC
                     TRQACAFRNAYDGFKKEDIQVIGISKDSIKSHQKFAEKHELPFILLSDPDLVAIKAFD
                     VWKEKKMYGKTALGVVRATYIIDENGIIEKVFEKAKPDTNAQEILEYLEKQE"
     gene            complement(14447..16144)
                     /gene="ade"
                     /locus_tag="KPD58_001158"
     CDS             complement(14447..16144)
                     /gene="ade"
                     /locus_tag="KPD58_001158"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBG0264941.1"
                     /translation="MGNLKRQCDVSSGREKADIVLKNGTIINVFTEELITGDVAIVGD
                     TIVGIGDYKGNVEIDCSNKYISPGFIDAHMHIESTMVMPIELSKKLLKSGTTTIIADP
                     HELVNVKGVSAIDFLLESTKDIPLNVYIMVPSSVPATSFETNGVGKFSAKDMESYVNN
                     PRILGLGEVMCFNDVINSENEILDKLELFKNKVVDGHAPNINGKSLQTYVCAGIENDH
                     ECITFDEVYEKLRAGLKILIREGSAAKNLKSIVSGMLKHNLPIEEFMFCTDDKHLDDI
                     EKQGHIRWNIKCAIDLGMEPVRAIKVATYNSAKAYGLRKIGAIGAGYKADIVVLNDLD
                     KMEVDSVYKDGNLVNEEMFSNYNYEIKDKELLNTVKFKYINKEKIQLKVSEKNYVMEI
                     VPYQILTNKVYESLPCADGYFVPNKEYSKLCVVERHRMTGNVAIAPLKGFGIKNGAIA
                     TTVAHDSHNIIVAGDNDDDILVAINYLKEIQGGYVIVSNGKVLAHLSLQVAGLISTFT
                     AEEVQEITDNMLEIARKMEVPEYVDPFITLSFMALPVVPQIRLTDLGLFDVEEFKFI"
     gene            complement(16450..17604)
                     /locus_tag="KPD58_001159"
     CDS             complement(16450..17604)
                     /locus_tag="KPD58_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic efflux protein"
                     /protein_id="HBG0264942.1"
                     /translation="MEMLISASEEAFLHVGSMIGFFILLFGYINYKTSGNFTNIISKN
                     RKFQPLIGALIGAIPGCGGSLAIMPLYINGKLSFGAIIASLIASMGDAAFVLISSNIK
                     MYFFVTIVSTITGIITGQLVDYFKLEEKLGLKNRRKSDKYDISKNNPKENKEQHNHDE
                     IILDTLAKSHGNTNRLAFIITHGKGYKIYIGIIVIGFIFMSLAHSGLNLPIIEKLHSL
                     EEVIAVIGILFSIIYMWCFKKVFKNSNQKEEENKKISLREMLIHSVGEISFVITWIFI
                     AYLIYDLIILILGGDEYLVKLVLSTGVISVFIGAGLGLIPGCGIQIVLMSFYLKGTIP
                     LGAVIANSISQDGDALFPLLAMDKKSSLWAMIITTIPAILVGMIVYAFLG"
     gene            complement(17849..18688)
                     /locus_tag="KPD58_001160"
     CDS             complement(17849..18688)
                     /locus_tag="KPD58_001160"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HBG0264943.1"
                     /translation="MNIKIAKNAGFCFGVKRAMKMAWDEVEKNDSGIYALGPLIHNKQ
                     AVAKYEEKGLKTVNEIDTIPNHENMIIRSHGVPENIYKEAKDKKLKIVDTTCPFVKKI
                     HTVVSEYHNKGYEIIVIGDMKHPEVIGINGWCENSAIIIKTLEQMENMEFDNSKRYCL
                     VAQTTINPELYISIVNKLSDKLEEIVFNDTICSATKTRQESAKELAKEVDCMIVIGGK
                     HSSNTQKLVKVCEDLVPTFAIETKDELDVNTLKKYKNLGITAGASTPNWIIEEVVTFL
                     ENL"
     gene            complement(18701..19297)
                     /locus_tag="KPD58_001161"
     CDS             complement(18701..19297)
                     /locus_tag="KPD58_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBG0264944.1"
                     /translation="MNFYRFVINIFKGFSKIFFKYEVIGAENIPDRGNIVIASNHKSN
                     LDPIFLAAAIENREIAAIAKKELFKVKPLGFILKKLHVMPINREKPDVSTIKTILRSV
                     RDGYVLGIFPEGTRIKGDSFGKAKAGLSVFTIKSKSKVVPVSIISKYKLFSKVIVYIG
                     EPISFEEHFKEKLSNDDHERISQEILEVIKQNYFKYSK"
     gene            complement(19348..19998)
                     /locus_tag="KPD58_001162"
     CDS             complement(19348..19998)
                     /locus_tag="KPD58_001162"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBG0264945.1"
                     /translation="MGNLVIAVDGPAGAGKSTIAKIVAKKLNINYIDTGAMYRAVTYK
                     CLKSGIDVNNEKEVIQIAENSDIDFKDNNIYLDKEVINEEIRTIEVSNNVSNVAKIKE
                     VRQLMVEVQRKIGMKNSVILDGRDIGSYVFPDADYKFFLVATPEERGNRRYKELCNKG
                     YNTTLEEVIEDIIRRDEIDSNREFAPLVKANDALEIDTTGKTIEEVVEEVVSKINL"
     gene            complement(20044..21297)
                     /locus_tag="KPD58_001163"
     CDS             complement(20044..21297)
                     /locus_tag="KPD58_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG0264946.1"
                     /translation="MSKVIVVGGGASGMMAALSASKNNNEVILVERNGELGRKLRATG
                     GGRCNFTNNREIEDFFDKVVSNKKFLYSSFYTFTNKDLISYFESRNLEYKIEEENDHK
                     VYTKNDKSIEVIEVLNKDLLNHNVKIMYNKKVIDIITEEIALKDDSNKDKSKYLIKGI
                     ILDNGDKILGDKVIISTGGVSYSKTGSDGSMYKILKKHGHTLNKLYPALVPLTIEEKW
                     IKDLQGISMKNVEISCKIKKRKISKSGDMLFAHFGITGPCVLIMSSYINKIIEKEKVE
                     LNIDFLPNLSTDEISSIIRAFPNKNVLNNLKQILPQNFLKEIFSLLSLVDKKASDLSK
                     ADEIRIIEYIKNMKLTCNGTTGINTGMVTSGGISVKEINSSTMESKLVKNLFFTGEVI
                     DIDAETGGYNLQIAFSTGYLAGISV"
     gene            complement(21321..21962)
                     /locus_tag="KPD58_001164"
     CDS             complement(21321..21962)
                     /locus_tag="KPD58_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBG0264947.1"
                     /translation="MGNTFYIVRHGQTDWNILGKTQGHGNSDLTPQGIEQAKELSEDI
                     GKYSIDYIFSSDLGRAMQTAQILGDKLNIEVQKTEALREMGFGVWEGLLIKEIQKDYS
                     DIYATWRNEPHLVNIPEGETLKIIKERVDAFIKELNEKYDNKNIILVTHSITLRVMLL
                     SFLESGMENIYRIKQDNTALNIVEFKDYGPVIVKMNDTSHIKNHVKINNSALE"
     gene            complement(22164..22577)
                     /locus_tag="KPD58_001165"
     CDS             complement(22164..22577)
                     /locus_tag="KPD58_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HBG0264948.1"
                     /translation="MLELKNNLGISERKLKYPCIYKHFKGKYYATMGLSKAIDDIENI
                     CEIYGKENLIQNRNKYKLVIRHTEREEDIYVYRDLDGNFYHKKEEDTNDLVLYKTLYD
                     DTGIFARPLDMFLEKVDTDKYVNSIQEYRFEEVYK"
     gene            complement(22728..23069)
                     /locus_tag="KPD58_001166"
     CDS             complement(22728..23069)
                     /locus_tag="KPD58_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HBG0264949.1"
                     /translation="MKEENFINVEYEDKRFSINSKEVLYIEKQEYEVKIYTESGNYEV
                     GENAPCIRKLLKEENFFKCSNSYIVNLDKCEFYLSNNCIFVNNNFIRILGSERKKKLK
                     KKLEKLYDYED"
BASE COUNT         7367 a         4149 c         2468 g         9347 t
ORIGIN      
        1 agttgctgga tatccataaa tccagtttaa ttctggcata tatctaaagt tcattccata
       61 tataccagtt ataattgtaa gtggtgctga tatggctgtt aacaaggtta attttgtaat
      121 taagttattt gttttctcac ctactctgga agaataaata tctaataatt tatctgctaa
      181 ctctcgtgtt gacaatgcga aattgtataa tttgtcaatt ccaaaatcta taccttgaaa
      241 attttctaaa ttatattttt ttacatttga atattttaaa tatcttatat tttccttaac
      301 tattctatcc ccaatataca aaagaggtct agtattctta actataattc ttgttaatcc
      361 tcttacatca cttattctag atacatgatt cttattaatg ttgtccatca tttcatcttc
      421 tatttgaaga attaactctt caactttttc caaagactca aaaccatttt ttataacttc
      481 tctaagaatc aagtagttta tcttaaatag attaataact ggattttttt caagttgaga
      541 gttcttcaaa ataatatttt ttacaaattc aaataaaaag tgttcttcat tcactacaac
      601 caatataaaa ttatctgata aatacatatt tacctcttct ataacagctt ctccatctct
      661 tagttcaaat gtatttaggc ttaaaaaatc gtaattatca aataaatcaa gtttggttat
      721 ttcatcaaat ttaagacaat cattaaaagt tatttcatct atatccaaag tttccttaag
      781 caattttaaa ttttttggag cacttaatat aagataagaa ttttctgcta cataaaattt
      841 atctctaaaa tcttcaatag tctctcttgt atttaaattc agtatataca tacatctact
      901 cctaaatttc aatttacatt ctaaaaattt tttataatcc ttactgtaat tattttacac
      961 tattttaata attattattt aatatttata ttaaatttat tataaggtaa gtgttaaata
     1021 catttattat ttaaccaaag tttttattac agattacact atatacttat tttaaaacat
     1081 atttcatatt attgatacta aaaaattatt gatactaaaa aagctaaaaa ataaaatgca
     1141 tgtgctaaac tgacaacaag cacatacact tcattcttat tttaactaaa ctatataatt
     1201 ttaatatata ttaataagca cataatctaa taattaatat attacaacta ttcatcattt
     1261 aatctgtatc ctacaccaac ttctgtaatt atatatcttg ggtttgcagg ttgtttttct
     1321 atttttcgtc ttaacgttgc cataaagacc cttaaagact tagtctcatt ccctataaca
     1381 ctaccccata tttcttttat tataaaatta tgagtaagca cttttccatg atgctttgca
     1441 agtaatgaaa gaatattata ttcaataggt gtcaaatgga cttcaacatc atcaacaata
     1501 acttttcttt tatcaaaatc aatcaataat ttatctactt caaacgtaga tttaacatca
     1561 tctttttgtt gagcttctac ttgtgaatgt ctaaatgcta ctcttactct tgctaataac
     1621 tctactatgg agaatggttt tgttaagtaa tcatctgccc ctgcatcaaa tacttctacc
     1681 ttgtctctat cttgctctct agctgaaact acaattattg gtatatttga aaattgtctg
     1741 atagctttta ttacctctat tccatccata tcctctaagc ctaaatctaa aagaacaaca
     1801 tctggagaat atgagacagc tatagatata ccttcttttc cagttatagc ctccttgaca
     1861 tcataatcct gtgttaaaag tgcagtagat ataaattttc ttattgtctt atcatcctct
     1921 actaacaata taagtttttt actcatttct tcctccaaaa ctaattttaa aatttaaaac
     1981 tattcatttt ccttaggcaa actaaactta aaagttgaac ccttatcttt attgttttct
     2041 accataattt cacctttatg agcttgaatt atagatttac aaattgataa tccaagtcca
     2101 acaccctttc tggaatcttt agctccttct tctccagtaa aaaatctgtc aaagatatgc
     2161 tttttaagtt cctttgatat accaggacca ttatcagata cttcaaacca aacataatca
     2221 tctttctcat atacatttat ctctatctta caatcttcct ttgaatattt caaagaatta
     2281 tctattaaat taattaaaac ttgttctatt aattttgcat ccataggaac aaataatatc
     2341 tgttctggta tattaatatc tataagtgca ttttcaattc ttttttttat tttttgtaac
     2401 gcttctgata taatttcctc aaccagttca gaatgttttt taacttccaa tttcccttca
     2461 tctatcttgg tcatactaag taaattttca acaagtctta ttaaccactg agtatcttca
     2521 tatacaccac taagtaattc atctattata tcttgaccta tagtaccctt gttttttata
     2581 attgtactta ctgcaccaga tattcctgct agtggagttc taaggtcatg cgaaactgct
     2641 cttaaaagat tacttcttaa tctttccctt tcaatctcaa gattggtatt ttctttagct
     2701 tctgacaaga tttctctatc aagcgctatt gccatttgag caattattgt ttcaattaag
     2761 cttatatctt ctgtatccaa ttttgaatca atacatgata ttcccactac tcctaaagtt
     2821 aaattcattc ctacaattgg tatatagtat ccctttgaac caggaagtgt atccgtgtca
     2881 tttcctgcca ctgttgaatt ggataaaacc caacttgcaa cagctttttc atcttcacta
     2941 tccaaatcat ttttatcctt attattagta ttttttttgt aaagctttga tttattatgc
     3001 ttatctacat ttaaataaca aattacatct ctacttaagc taagagacaa aagttctata
     3061 cctttgttta ctatatcatc tttatttgat aatcttaaaa atcccttaat tatttgatat
     3121 atcatttttg tattttcttc tcttttagca gcagtttctg cttcatgctg tatcttagac
     3181 gtaagagtgc tagttattat acctactatt gagaatacaa ccagtgttac tatatagttt
     3241 gaatctgcta tagataatgt atataatggt gctgtaaaaa aataatttaa aagaactata
     3301 ttaaatacag aacttattat acctgtacta taccctttag tccacataga aactaaaact
     3361 acacccaaca tatatattaa caatatattt tctcttataa atcccatgct ttgtactgca
     3421 tatgctaaaa tacttatcat caaagtaata aatataagtt ttataaaatc aactttgcta
     3481 attttaaatt tagattttat tgatgattta tcctttttag gtttgtattt aacatctgag
     3541 gcaaatttat atggaattat atgtatatct atataatcaa cttcatcaat taatttgtca
     3601 actatatctt ttttaaatct tttactaaat cttttattac ttgaatgatt tctacctatt
     3661 acaatttttg ttacatttct cagttttgca attctaagta tttgctctat tatattttca
     3721 ccatgtaaaa caattatttc tccacctaat ttcttagcta attttaggtt attttgtagt
     3781 tgtttttgtt catcaaatgg aagtcttgct gtgtttggag tttcaacata taaagctatc
     3841 cattttgcaa atgtactgtc tgatattctg gatgcagttc taataacttt tgctgaagat
     3901 ggagatggac ttatacatgc aagaagtgta tctgatgtag gtattacagt tacatcaccc
     3961 tttgatagtc tggtcatttg aacttcttta ttaactctct ctgctgtttt tctaagtgcg
     4021 atttctctaa gtgcaaccaa gttatctttt ataaaaaaat tattttttgc tctagtagct
     4081 tgttctttct tatatatctt accttcattg aacctttcaa gaagtacatc aggttcaaca
     4141 tctataagtt ctaactgtgt atttgtatcc aaaaacttat ctggtatagt ctctttaact
     4201 gatacgtttg taattatctc aacaatgtca tttagacttt ctaaatgttg tacatttaca
     4261 gtagtgtaga catttatgcc tgctaacagc aattcttcta tatcttgcca tctttttgta
     4321 tgccttaacc cagcaacatt agaatgggca aattcatcta ctaatataac ttctggtttt
     4381 cttgctaaag cagcatctaa atcaaattct tttagtttta tagatttata ttctatatcc
     4441 tttacaggaa gaatttccaa gccatcaagt aaagccaatg tttctggtct agtgtgtggt
     4501 tcaatatatc caactactac atccacaccc acttttttta aattttgtgc agattccaac
     4561 atagagtagg tttttcctac tccagctgca taaccaaaga atatttttaa ttgacctcta
     4621 cttagttttt cttcttcatt tttaattttt tctaatagaa tatcaggatt aggtctatcc
     4681 attaaaaaac ctccttacac cattcattac tattataata tcaattccga tataaaattg
     4741 tagttaagat gtatttttaa tgattaagat tatgtaaaga tttaaatatg aatttaaaaa
     4801 atttagttgt aaaataaaaa tgcctaacaa taggcatttt tatcttgtgt ataatcattt
     4861 tagtttaagt taagtatttc cataaactca tcacctgtaa tagtttcttt ttctaataaa
     4921 taattagcaa gttcatgcaa tttatcaata ttattttcta agatttcaat tgcatttttg
     4981 tgacaagcct ttactatttt aaatacctct tcatctattt tagcagcagt gtcggaagaa
     5041 caagcaagag aagtatctcc tcctaaatat tgattgctta cagtttcaag agcgaccata
     5101 tcaaactctt cactcatacc aaggcgagta accatagctc ttgcaatttt agtagcttgt
     5161 tctatatcat ttgatgctcc tgatgtacaa gtgttaaaga taacctcttc agcagcacga
     5221 ccacctgtaa aagtagttat tttatcaatt gcttcttctt tagacataag tacactctca
     5281 tcttcagcaa cttgcatagt atatcccaat gctcctgaag ttctaggtat tatagtaatc
     5341 ttatggacag gtgcagaatg tttttgcata gcagcaacaa gtgcatgacc tatttcatga
     5401 tatgctataa ttttcttttc cttatctgaa attacagctc ccttacgttg atacccagca
     5461 attacagttt caacagattc ttcaaaatcc tgttgttcta ctatatctct gccagactta
     5521 actgctaaaa gtgctgcttc gtttacaata tttgcaagtt ctgcacctga tgctcctgat
     5581 gtagaccttg caattgcatt ataatctata tctgagctta catttacatt ttgagcatgt
     5641 acctttaata tagcttcacg tcctccaagg tctggaagtt caactggtat acgtctatca
     5701 aatcttcctg gacgaagtaa tgctttgtca agactgtcag gtctatttgt agcagcaagt
     5761 attactactc caattccacc atcaaaacca tccatctcac ttaacaactg atttaaagtt
     5821 tgttcacgtt catcatttcc acctaatcca cttccattat cacgcttttt accaatagta
     5881 tcaatctcat ctataaacac tatacatgga gctttttctt ttgcttgttt aaataaatca
     5941 cgaatcctag aagctcccat tcctacaaac atctcaacaa actcagaacc agatattgag
     6001 aaaaatggta catttgcttc acctgctaca gcttttgcaa ggagtgtttt tcctgtacct
     6061 ggaggtccta ctaaaagtgc tcctttaggc atttgtgctc ctatcttttt atacttttct
     6121 ggattgtgca agaaatctac tatttcagtt aaagcttctt ttgcctcatc ttgaccagct
     6181 acatcattaa atctttttcc actttgagca gatacataca ttttggcgtt gctttttcca
     6241 aattgcatag cattaccacc cattcgcttt tgcatagact tcatcattaa gttaccaaat
     6301 agcataagaa tacctatagg tattatccat gtaagtaaaa tacttaaaag aggtgaattt
     6361 tccttaggaa taaccttagt aaattctact tttgcatttt ttagtctatc taccaattct
     6421 gggtcatcca ttctaccagt tatgtatata ttcttatcct tgctactaga tggatttatt
     6481 gctattctat tattttctat ctcaacagat ttaacctttc ctgtatccaa cattgtgaga
     6541 aactttccat agtcaatctc aaccattttt ttatccatta aacttggaaa tattaaagca
     6601 tttaaaaaca ttactactat tattataact gtataataat agagtgctga ccttttttgg
     6661 ggtttatgtt catccataat tttattctcc tttaaattat ttttatgtta tttgcaatca
     6721 atgtaagtta aaagtttatt taatttctct aaaccttcta ttatatcttc catatcttgc
     6781 aatttttcac tggtcatatt ttcaaaataa ctatcaaaaa tactacttga aatatgttct
     6841 tgtatatcca atgatttatt tgtaactttt acttttacaa ttcgttggtc ttctatatct
     6901 cttatgcgat ttaaaaagcc atttttttca agcctttgac agattactga tacattacta
     6961 tttgtcatgt ttaagttctt ccctaaatcg cttatttttt tctctccatg ttcacataac
     7021 tcagataaaa tcaaagcttg cactgctgta ataccaagtg gctgataaaa atcacttaat
     7081 aaagttgtaa atttcatatt tatatctcta gtcaactcta gtagctcttt cttaaaactc
     7141 atctcatcca tacaaacgct ccctacgaaa atactttatt tcaaatttag gctgaagtgc
     7201 atactgtaaa taaatactta acactataat gtttaatgta ttaaatatta tagtattaag
     7261 tatttattgt caattacaat attgtaactt caattggaat tttgttatct tattgatatt
     7321 taattttata caggatattt ttaataaaat ttaataaaaa atctatatat atattttaca
     7381 ctgatttctc cctttttaat ctcttttaaa agttaaaata caaatttatt gatactataa
     7441 aggtttagtc tcccattatc gcaccatata taaaattaaa gccacctcta tctacataca
     7501 cctaaagtga ttttcttaaa atataaaact actattataa ggtcaaattt aaaaaaaata
     7561 aggtataaag tatcattaag attaacttta tacctataaa tttctatatt tttttagtta
     7621 acatttatat actcttaaaa tttattgtat caaatagttt tttatccaat ttctaaacaa
     7681 caattctgaa tgttttttcc attttacagt tggctcttct gaagggtcat cattaatata
     7741 gtaattttga ggtatagata ttttatctcc tttatttata tccctcttat attctttatc
     7801 aagagtgtat ttgtcatact cgctatgacc cattacgaac acatctcttg aatttgtaat
     7861 tatataggga ccagcttctt tagaatgact cacaatgctc aactctttat tctctttcaa
     7921 agcttcaata tttacagtag tatgtcttga atgtggagca aaaaattcat tttcaaatcc
     7981 atctactatt ttactatctt tatctacctt atgcttaaat actccaaaac atttttgact
     8041 tagtggaagt ttttttacat tgtagtattt gtaaagactt gcttgagctg cccaacatat
     8101 atataatgta gatttagttt ttctcttaga ataatccata atttcttcta gttcattcca
     8161 ataatcaact tcttcaaact ccatttgttc aactggagct cctgttatta taaagccatc
     8221 aaaatactct cctttggctt catcaaatgt tttataaaat tctttaacat attcattaca
     8281 acttctttta ctttctcttg ttgaaatttt aataaattcc acagacacgt tgaatcctgt
     8341 tttatctatc cttcttagta aatctaattc tgtatctatc tttataggca ttagatttag
     8401 tatagctatc ttagtgtcta tgttttcttc atactttaat tcctttttaa aatcttcttt
     8461 ataaattaca tctattcctt catctaataa tttattaata acaggtaatc cctttggtag
     8521 tattaaagcc atatcttttc acttctcttt ctcttagaat attagaaatt taaagcttga
     8581 tttaaatcag ctattaaatc ctctacattt tctattccaa cagataatct tattagtgaa
     8641 ggcaatactc cagatgcaat taattgttct tctgttaatt gtctatgagt agttgaagct
     8701 ggatgtataa cacaagttct tacatctgaa acatgtgtaa ccaaagatgc tatctgtaat
     8761 ttttccacaa attttttggc attttctaac ccacctctta ctccaaatga taaaacacca
     8821 ctacatcctc ttgataaata ctttttggca ttctcataat acttgttatc ttcaagtcct
     8881 gggtaattaa tccaatctac attttcatgt ttttctaaaa atctagctat tttaagtgca
     8941 ttttcactat gtctctccat tctaagatgt agtgtttcaa catttaaatt agtaagatac
     9001 gcattgaatg ggcttaaaca atttccatag tctctaagca actgaactct tgctttagtt
     9061 gcatatgcgg catttccaaa tttttgagta tagcttatac catgatatgt tgggtctggc
     9121 tcaactaatt ctggaaattt tccattatcc cagttaaagt ttccaccatc cactataatt
     9181 cctccaacac ttgaagcatg cccatctaaa tattttgtgg tagaatgagt aactatattt
     9241 gctccatact ttaaagggtt acaaagcact ggagatgcta atgaattatc aacaataaat
     9301 ggaacattaa ttctcttagc tacatttgct attttttcaa aatctatgac ttcaagagtt
     9361 ggatttgcaa gtgtttctgc aaacacaacc ttagtatttt cctttgcaag ttctactatc
     9421 tcatcttcac ttgaatctaa gtcaaacgat attaaatcta taccaaattt tttaagacta
     9481 ggtcctagta aattgaatgt tcctccatat acttttgaag aacaaagtat actatctcct
     9541 gatttacata tatttaaaac tgccaacata tttgcagatt gccctgatga tacagctaca
     9601 gaagatactc caccctctag taaacttatt ttttcttcaa aagcttgaat agtaggattg
     9661 cttattcttg aatatatatg tccatcaact tttaaatcaa ataattcagc aagttggtct
     9721 atactgctat atttaaaagt tgtactttga tataaaggta gtactcttgg ttctccatta
     9781 cctggtttat aatttccttg cacacatatt gtttctttat tatacattct tgtatccccc
     9841 ttagatttta gtaaaaaaaa ttaaactatt taccagatga gagttaaaat aaataaagcc
     9901 tgagtatata actcaggctc tgtataagct taattatact catctctcag ctttcgccgc
     9961 aggatttagc accaatgtat ataaatatac tggttgccgg gtttcatagg gccagtccct
    10021 ccacctctct ggataagatt tatttaattt ttctatgttt tatatcttat atatttttcc
    10081 taattttgtc aactattttt ttaatttttt tcttttaata attttcccaa cctctacttg
    10141 aattacagac attatggcta aaacaatcgt tataagccac tctaagttat ttaaaactgt
    10201 taagttaaaa gcatttctaa aaacaggtac aaacagtatt actgctatta aaaatgcaga
    10261 agttaaaaat gagattaaaa gaaatggatt tttaccatta tttctattaa acattgaatc
    10321 tgtatttgaa cgctgactaa atgctcgtaa catctgtgaa aatgctaata cacaaaatgc
    10381 cattgtttga cctacagaat gattttctat attaagacca attttatatg ctaaaatagt
    10441 cattattgcc acaaatacac cttgggtaac aactctttgt ataagatttt tttcaaacaa
    10501 agtggttgat ttcactggct ggtgcttcat tatatttctg cttggtgggt caactccaag
    10561 agcaagggct ggaagcgtag cagttgcaag gtttacccat agaatatgaa cagcaagaag
    10621 cggagtatcc caatttaata atattgctac aaacaacgta agtatctctg ctatattacc
    10681 aacaagcaaa aattgtataa ctttttgtat gtttctatat acacgtctac cttcttttat
    10741 tgcataagca attgttgtaa aactatcgtc cattaaaatc atgtcagaag aatcttttgc
    10801 aacatctgta ccacttatac ccatggctat accaatatca gcagacttta aggcaggaga
    10861 gtcatttaca ccatccccag tcattgctgg tacttctcca attcttttta gagattgtat
    10921 aattctaagt ttatcaaatg gggatacacg agcaaatact gtagtatttt taacagcttc
    10981 atctagttca tcatcactca aattatcaag ttcatcacca gatatcacag tattttcttc
    11041 actccatatt cctaactcat gagcgattgt tgtggcagta actttatggt cacctgttat
    11101 cattatggtt cttatacctg cttctttaca agtacttact gcatcaatta cctcagttct
    11161 tggtgggtca atcataccaa ccatacctat aaatgttaaa tcatattcta tattttcact
    11221 atcttctttt ggtatagatg aaatctcacg cttagcaaaa ccaagtacac gcaatgcatc
    11281 tttagacatg ttaagacata attcgtgtat attcttttta tccacttcag taatgtttct
    11341 ttcacctttt gaagttaata ttttagtaca taggtcaaga atttcgtcta ctgctccttt
    11401 agtataagca ataatctttt catctatatt atgaacagtt gtcatacatt ttctatctga
    11461 atcaaaaggc tgttcaaata cacgtggata ttcttcttca agagcttctt gatctattcc
    11521 aaattttttg gccataaaaa ttaatgcgcc ctctgttggg tcacctaata tttcatctgt
    11581 tttatctgga ttaatacatg catcattaca aagtgcacca gcataaacaa tctctttata
    11641 cactaatgga tgtttttcag atgcagtatc tattgatgtc acttttccag attcaaaatc
    11701 tccattgaca gcaatatgtg taacagtcat tttatttaag gttaatgtac cagttttatc
    11761 acaacatatt actgtagctc ctcctaatgt ttcaacagca ggtaattttc taattaaagc
    11821 atttctttta gccatacgct gtacgcctaa agccattaca atagtagctg ttgcaggtaa
    11881 tccctctgga ataattgata tggcaaggga aattgcaatc ataaacattg gcaagagagg
    11941 tctctgataa aatgcaccta ttgcaaatat aacaacgcat acaataatac ctatgacact
    12001 taatgtcttt ccaacagtat taagtttacg ttttatagga gtatcaaaat catcttgtga
    12061 atctaaaaga tgtgctatgt tacccacttc tgtatccata ccagttgcta ctacaacacc
    12121 tacacctcta ccatatgtga ctattgaaga tgtatacgcc atattgctac ggtctcctaa
    12181 tacacattct ttaggtaaga ttgtatctgc atctttttct gatggaacag attctcctgt
    12241 taaagaagcc tcttgaattt ttaaacttga tgtttctatt aaacgaaggt ctgctggaac
    12301 catagctcca tcttctaaaa aaacaatatc tcctactaca agattacttg ctggaataac
    12361 attttcttca ccatcacgca taacacgagc tgtaggtgaa ttcatatttt ttaatgcttc
    12421 taatgatgat tctgcttttt tctcttgaac aattccaata actgcattta ctgttacaat
    12481 tattagtatt acaaaagctt ctgtaaattc gccaaatatc aaagataata ttgctgctcc
    12541 tattagtatc ataaccatag catctgtaat ttgactccaa agcatttgta aagttgttct
    12601 aggtggtttt tttctaagtt catttaagcc attctcttta agccgttttt ccgcctcggt
    12661 atcggtgagc ccctcctctg atgaatctag actttctaaa atttcatgcg ttgctcttga
    12721 atgccaaggc atctgtttgt gattttccat tttggaagac cactcctttc tttttttaat
    12781 ttaagaataa ttatatttta aaatcaagtt caattcaata cacactaagt atattttacc
    12841 taaatttaac aatgccattt aatatttaac aaaacttcta ctcttttaca aatatgtatt
    12901 cactattgtt agataagttt tctttatact tataaccttc taaatcaaac tttttcagtt
    12961 cttcaatatc tcttatccta ttttttacaa tgtattttgt catcatacct ctagctttct
    13021 ttgcttgtgt agattccact ttcaatatgc catttttatt tactttaaaa gtacatgtaa
    13081 tatatgtatc actatcttta ataaattttt ctatggattt gctgtattca tttgatgaaa
    13141 gattaacgat agctgttttt tctttgatta gttctttata tatacgattt ccccaatatt
    13201 cgtataaatt cttaaactca cctacttgta attttgtctg catttctaat ctatattcat
    13261 atatactatc atatggtttt acaacaccat acaatccact tatgattcga atacagctat
    13321 ttgcaaactc ttcatctttc cttgtaaaat tttctgcttc tatattttta tattgtattc
    13381 catcataagt taagattgca gggtttccta atctatcaaa tttaatgctt tcaaatctat
    13441 ttttatttaa tacagctaat ttttcattaa ttttcatttt atttttaaaa tcttctatac
    13501 caaatgtctt tatattttcc actatttctt ttgtgttatc tataaagcaa ggcaaactca
    13561 aatctctatc aaatacttct atattcttca tatttttagc tggtgataac attataatca
    13621 taaattctta ttactcctgt ttttccaaat attcaagaat ctcttgtgca tttgtgtctg
    13681 gttttgcttt ttcaaagact ttttcaataa ttccattttc atctataata tatgtcgcac
    13741 gtacaactcc caatgcagtt tttccataca tttttttctc tttccataca tcaaaagctt
    13801 ttatagcaac taagtcaggg tcagaaagta aaataaaagg aagttcatgc ttttctgcaa
    13861 atttttgatg tgattttata ctatctttac ttatcccaat aacttgtata tcttcctttt
    13921 taaatccatc atatgcattt ctaaaagcac aagcttgacg tgtacatcct ggtgtattat
    13981 ctttaggata aaaatatact actacttttt tgcctttaaa atctgacata cttaccttat
    14041 ttccatcttt atcttctagt gtaaattctg gtgcttttgt tcctatactc aacataaata
    14101 tctctccttt tgtttttatt aataatttta tactatgctt atattattac cctataaatc
    14161 tatatataaa taaaatattt tattttttat gtataaagtt tcataaatat ataattttca
    14221 tgtatttata tgttaaaata gactgaaaat agttttatta agatatctat gcttaaaaca
    14281 atgttaaatg atagattaat taaaataagg atatctaaaa ataaaatttt tagttcatga
    14341 gatgctcttt tttgtatgaa atcaaatata ttttcatcat ttcaacaata aaaagagact
    14401 tatctctatt ttgagaaagc ctcatttatt attactttta tactatctat ataaatttaa
    14461 attcctcaac atcaaataga cctaaatctg taagtcttat ttgtggaaca actggaagtg
    14521 ccataaaaga aagtgtaata aaagggtcta catactctgg aacttccatt tttcttgcta
    14581 tctccaacat attatcagta atttcttgta cttcttcagc tgtaaaagta ctaattaatc
    14641 cagctacttg aagacttagg tgtgcaagaa cttttccatt tgatacaatt acataaccac
    14701 cttgtatttc ctttaagtaa tttatagcta ctagaatatc atcatcatta tcccctgcaa
    14761 ctataatatt atgagaatca tgggccacag tagtagcaat tgctccattt tttatgccaa
    14821 atccttttag tggagcaatt gctacatttc cagtcattct atgtctctca acaacacaaa
    14881 gttttgaata ctctttattt ggaacaaaat atccatctgc acatggtaaa gattcatata
    14941 ccttatttgt caaaatttga taaggtacaa tctccattac ataatttttt tcacttactt
    15001 ttaactgtat tttttcttta ttaatatatt taaacttgac tgtattaagc aattctttat
    15061 ctttaatctc ataattgtaa ttagaaaaca tctcttcgtt taccaaatta ccatctttat
    15121 atacactatc cacttccatt ttgtctaaat cattcaatac aacaatatct gccttataac
    15181 ccgcacctat tgctccaatt ttccttaacc catatgcttt tgcagaatta taagttgcaa
    15241 cttttatagc cctaacaggt tccataccta aatcaatcgc acattttata ttccatctta
    15301 tgtgaccttg tttttctata tcatctaagt gcttatcatc tgtgcaaaac ataaattctt
    15361 caattggcaa gttatgcttt agcataccac ttactataga ctttaaattt tttgctgcgc
    15421 ttccttctct tattaaaatt tttaatcctg ctctcaattt ttcatatact tcatcaaaag
    15481 ttatacattc atggtcattt tctattcctg cacatacata tgtctgaagt gatttaccat
    15541 ttatattagg tgcatgtcca tctacaactt tatttttaaa aagctctagt ttatccaaga
    15601 tttcattttc agaatttata acatcattaa aacacattac ctctcctaaa cctaaaattc
    15661 taggattatt tacataactc tccatgtctt ttgcactaaa tttaccaact ccattagttt
    15721 caaaacttgt tgcaggtacg gatgaaggca ccataatata tacattaagt ggtatatctt
    15781 ttgtcgattc taataaaaaa tctattgcac taactccttt tacattgacc aactcatgtg
    15841 ggtcggctat gatagttgtc gtacctgatt ttaataattt tttcgataat tcaattggca
    15901 ttaccattgt agattcaata tgcatatgtg catcaataaa gccaggtgat atatatttgt
    15961 tactacaatc aatttctaca ttgcccttgt aatcgccaat tccaacaata gtgtctccta
    16021 ctattgctac atctcctgtt attaactctt ctgtaaatac atttatgatt gtcccatttt
    16081 ttagtacaat gtcagctttt tctcttccac ttgaaacatc acactgtctt tttaaattac
    16141 ccatttttac aactcctcct aagtttttta tatcaatata ctgactgtat tacgtattat
    16201 agtttaattt attaatatat attatatatg agttttgata gaaagtatca cttgaaattt
    16261 gttccataat tcctttaatt aaatagagag aacatgagga ataatattat ctcccatgtt
    16321 ctcattaata tttaagcaat aatttaattc atattgaatt ttttattttt atatttaatc
    16381 ttcactattc ttataaataa atttatgtgc tttattattt ttttaatact tctttattca
    16441 taattatatt taacctaaaa atgcataaac tatcatacct acaagtattg ctggtatagt
    16501 agtaataatc atagcccata aagaagattt tttatccatt gctaaaagtg ggaaaagtgc
    16561 atctccatct tgagaaatag agttggctat cactgcacca agtggtatag ttcccttcaa
    16621 ataaaatgac ataagcacaa tttgtatccc acaacctgga ataagtccaa gacctgcacc
    16681 tataaataca cttattacac ctgttgataa tactaattta actaagtatt catctccacc
    16741 taaaattaat attatcaaat cgtaaataag atatgcaata aaaatccatg taattacaaa
    16801 tgatatttct ccaacagaat ggataagcat ttctcgcaaa gaaatctttt tattttcttc
    16861 ctctttttga ttagaatttt taaatacctt tttaaaacac cacatataaa ttattgaaaa
    16921 taatattcca atcactgcta ttacttcttc caatgaatgt aacttctcaa ttataggaag
    16981 attaagtcca gagtgagcaa gtgacataaa tataaaaccg attactataa ttcctatata
    17041 aattttatat ccctttccat gtgttattat aaatgcaagt ctatttgtat ttccatgaga
    17101 tttagctaga gtgtctaaaa ttatttcatc atgattatgt tgttctttat tttctttagg
    17161 gttattttta cttatatcat atttatctga ttttctacgg ttttttaacc ctaatttttc
    17221 ttctagttta aagtaatcca ctaattgtcc agttattatt cctgtaattg tactaactat
    17281 agtgacaaaa aaatacattt taatatttga tgaaataagt acaaatgctg catctcccat
    17341 actagctatt aaacttgcaa ttattgctcc aaagcttaat tttccattta tatacaatgg
    17401 cattattgcc aaacttccgc cacaacctgg tatagctcca attaaagcac ctattaaggg
    17461 ttgaaatttt ctgttttttg atatgatatt agtaaaattt ccactagttt tataattaat
    17521 atatccaaat aacaaaataa aaaatccaat catagaacct acatgtaaaa acgcctcttc
    17581 agaagcggat atcaacattt ccattaattt acctcctaga ctaattttgc aacaattata
    17641 ttatattcta ttttctattg ataatcaata tcaatatttc ttttgaattt ctaatattaa
    17701 aaagtgcgca tcttatactc ttagtttttc atcttaagat acacactttt tgatattagg
    17761 ttatctattt accacatgta atatgaaatc aaaggtggat attatgataa attataacaa
    17821 tgtccaccta tttttattac ctatcaaatt ataaattttc aaggaatgtt acaacttctt
    17881 ctattatcca attaggtgta gatgcccctg ctgtaatacc caaattttta tattttttaa
    17941 gcgtatttac atccaattca tctttagtct ctattgcaaa agttggtact aagtcttcac
    18001 aaacctttac taacttttga gtatttgaac tgtgtttacc tcctataact atcatacaat
    18061 caacttcttt agctaactct ttagctgatt cttgtcttgt ttttgtagct gaacaaattg
    18121 tatcattaaa tactatttct tctaatttat ctgatagttt attaactata cttatataca
    18181 actctggatt tattgtagtc tgtgcaacaa gacaatatct tttggagtta tcaaattcca
    18241 tattttccat ttgttctaag gtctttatta ttatagctga attttcacac catccattta
    18301 tacctataac ttctggatgc ttcatatctc caataactat tatttcatag cctttattat
    18361 gatattcact tactactgta tgtattttct ttacaaatgg acatgtagta tccacaattt
    18421 ttaatttttt atctttggct tccttatata tattttcagg aactccatga gaccttatta
    18481 tcatattttc atgatttggt attgtatcaa tttcattcac agtcttaaga cctttttctt
    18541 catattttgc tactgcttgc ttgttatgaa taagaggacc taaagcatat atgccagaat
    18601 catttttttc aacttcatcc catgccattt tcattgctct ttttactcca aaacaaaagc
    18661 cagcattttt tgctattttt atgttcataa ctttaccccc ttattttgaa tatttaaagt
    18721 aattttgttt tatcacttct aaaatctctt gtgatatcct ttcatggtca tcattactca
    18781 atttttcctt aaaatgttct tcaaatgata ttggttctcc aatatataca attactttac
    18841 taaaaagttt atatttagat attattgata ctggaacaac tttagattta ctttttattg
    18901 taaatacaga aagacctgcc ttagccttac caaaagaatc tccttttatt ctagtaccct
    18961 ctggaaatat tcctaataca taaccgtctc taactgacct taaaatagtt tttattgtag
    19021 atacatctgg tttttctcta tttataggca taacatgcaa tttttttaat ataaatccca
    19081 atggttttac tttaaacaat tctttctttg caattgcagc tatttctcta ttttctatgg
    19141 cagcagccaa gaatataggg tcaagattcg atttatgatt tgatgctatt actatatttc
    19201 ctctatctgg aatattctct gcacctataa cttcatattt aaaaaatatt ttagaaaaac
    19261 ccttaaaaat atttattaca aatctataaa aattcacgtt tatctccctt caacactaca
    19321 tttattttca ttatgatgca taaaaaacta aagatttatt ttagatacaa cttcttctac
    19381 aacttcttct atagtttttc ctgtagtatc tatttctagt gcatcattag cttttaccaa
    19441 tggtgcaaat tctctatttg aatcaatttc atctcgtctt attatatcct caattacttc
    19501 ttctaaagta gtattatatc ctttattaca aagttcttta tagcgtctat ttcctctttc
    19561 ttctggagta gctactaaaa agaatttgta atctgcatct gggaaaacat atgaacctat
    19621 atctctacca tctagtatta ctgaattttt cattcctatt tttcgttgaa cttcaaccat
    19681 caactgtctt acttctttta tttttgctac attagaaaca ttattgctta cttctattgt
    19741 tcttatttct tcatttataa cttctttgtc caaatatata ttgttatctt taaaatctat
    19801 atccgaattt tcagctattt gaataacttc tttttcatta ttgacatcaa taccactttt
    19861 taaacactta taagtaactg ctctgtacat agcacctgta tctatataat ttatatttag
    19921 cttttttgct actatcttgg ctattgtact ttttccagct cctgctggac catctacagc
    19981 tataactaaa ttacccatat atacacctct atttattcat atttaattat taaaaaatta
    20041 tatttataca cttatacccg caagatatcc agttgaaaat gctatttgta aattatatcc
    20101 accagtttct gcatctatat caattacttc acctgtaaaa aataagttct taactaattt
    20161 agattccata gtagaagaat taatttcttt aactgaaata ccaccagaag taaccatacc
    20221 agtgtttata cctgtagtcc cattacaagt tagcttcata ttttttatat actcaataat
    20281 tctaatttca tctgccttag acaaatcact tgccttttta tcaactaaag ataatagaga
    20341 aaaaatctct tttaagaaat tttgaggtag tatttgctta agattattta gaacattttt
    20401 atttggaaat gctctgatta tactagagat ttcatcagta cttaaatttg gtaagaaatc
    20461 tatatttaat tctacctttt ccttttcaat aatcttgtta atataagaag acattataag
    20521 aacacaaggt cctgttatcc caaaatgggc aaatagcatg tctccagatt ttgatatttt
    20581 ccttttctta atcttacaag atatttccac attcttcatg gatattcctt gtaaatcttt
    20641 aatccatttt tcctctattg taagtggaac taaagcagga tatagtttgt ttaatgtatg
    20701 tccatgcttt ttaagaatct tatacattga accatctgaa cccgtctttg aataactcac
    20761 tcctccagtt gatataataa ccttgtctcc tagtatttta tctccattat ctaaaataat
    20821 cccttttatt agatactttg acttgtcctt attagaatca tcttttagag caatttcttc
    20881 agttataata tctataactt tcttattata cattatcttt acattgtgat tgagtaaatc
    20941 tttatttaat acttctatta cttctataga tttatcattt ttagtgtata ctttgtggtc
    21001 attttcttct tcaatcttat attccaaatt tctactttca aaatacgaaa taaggtcttt
    21061 atttgtaaat gtataaaaac tactatagag aaatttttta ttactgacaa ctttatcaaa
    21121 aaaatcttct atctctctat tattggtaaa attgcatcta ccgccaccag ttgccctaag
    21181 ttttcttcct aattctccat ttctctcaac taatattact tcattattgt tttttgaagc
    21241 tgacaaagct gccatcattc cagaagctcc tccaccaact actataactt tactcataaa
    21301 tttctcctta taattgattc ctactccagt gcagaattat ttatctttac atgatttttt
    21361 atatgacttg tatcattcat ttttactata acaggcccat aatctttaaa ttctactata
    21421 tttaaagcag tattatcttg cttaattcta tatatatttt ccatacctga ttctaaaaat
    21481 gacaacaaca taaccctcaa tgtaattgaa tgtgtaacaa gaattatatt tttattatca
    21541 tatttttcat ttagttcctt tataaaagca tctactcttt cctttataat cttaagagtt
    21601 tcgccttctg gtatatttac taagtgtggt tcatttctcc atgttgcata tatatctgaa
    21661 taatcttttt gaatttcttt tattaaaagg ccttcccaaa ctccgaatcc catttctcta
    21721 agtgcctcag ttttttgaac ttcaatattg agcttatctc ctaaaatttg agctgtttgc
    21781 attgctctcc caaggtcgct actaaaaata taatcaattg aatacttgcc aatatcttca
    21841 gaaagttctt ttgcctgttc tataccttga ggcgtaaggt cagaattacc atgaccttgt
    21901 gtttttccca atatattcca atccgtttgt ccatgtctta ctatataaaa agtatttccc
    21961 atatgtcctc ctaatatatg cctatttttt attaaatata aattatcact tctcaaaact
    22021 taattatatc atataatact ttactttgtt tctattttat aatctaatct aacatattat
    22081 ttacaataaa tctcatttgt atttctgcaa attattgtac tgttaaagaa tttttctaaa
    22141 tttataaaca tatttctctt atattattta tatacttctt caaatctgta ttcttgaatt
    22201 gaattgacat acttatctgt atctactttt tctaaaaaca tatccaaagg tcttgcaaat
    22261 attcctgtat catcatataa ggtcttgtat aaaactaaat cattagtatc ttcttctttt
    22321 ttatgataaa agtttccatc taaatcccta taaacataga tatcttcttc tctttcagta
    22381 tgtctaataa ctaatttata tttatttctg ttttggatta aattttcttt accatatatt
    22441 tcacatatgt tttctatatc atcaatagcc ttgctaagac ccattgtagc ataatatttc
    22501 cctttgaaat gtttatagat acaaggatat tttaactttc tttcactgat acctaagtta
    22561 ttctttaatt caagcatttt attataatct cccatatctc gtccctttct tttaaaaagt
    22621 attttattct gaaataagtt acttgtttta tattttatca taatttaaaa tttattaata
    22681 atacaatcaa taaataaaga ggctgtataa aatacagcct cccaatatta atcttcataa
    22741 tcatataatt tttctaattt cttttttaat ttcttttttc tttctgaacc taaaattctt
    22801 ataaaattat tattcacaaa aatacaatta ttgctgagat aaaattcaca cttgtctaga
    22861 tttacaatat aactattact gcatttgaaa aaattttctt ctttcaatag ttttcttata
    22921 caaggagcat tttctccaac ttcataatta ccactttcag tataaatctt tacttcatac
    22981 tcttgctttt ctatgtacaa aacttcttta ctattaattg aaaaacgttt atcctcgtat
    23041 tcaacattaa taaaattttc ctctttcatt tccatccctc caagtacttt tattaattta
    23101 attttagtgt tttttatcaa tataatctat cggaaatata tgtaagactg atttacttaa
    23161 gatatttaaa taattattat taatattaat gtataagttt attttttaac attaattttt
    23221 aaagttaaaa agactttttt taattatatc cgtatatata agtatatcta tctttaataa
    23281 aataactttt atagaaattc atgtaaaatt atacataaaa aagtaattat t
//
LOCUS       DAELLV010000035        23144 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.035, whole genome shotgun sequence.
ACCESSION   DAELLV010000035 DAELLV010000000
VERSION     DAELLV010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23144
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.035"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(74..760)
                     /locus_tag="KPD58_001167"
     CDS             complement(74..760)
                     /locus_tag="KPD58_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBG0264950.1"
                     /translation="MFGVIANSLAIAGGCIVGLIIKGGLPQRVSDTIMNGIALCVLYI
                     GISGALEGKNTLVTIISVAVGALIGELIDIDKWVNKLGAFLQSKFSKGNKKDSIAEGF
                     ISSSLLFCIGAMAVVGSLESGLTGSHDTLFVKSVIDGIASIIFTASLGIGVMFSAISV
                     FLYEGIICVGASFLNGFLSDPVVTEMTAAGSLLIIGLGLNVLKLTNIKVANLLPAIFI
                     PILFGVFGII"
     gene            complement(809..1342)
                     /locus_tag="KPD58_001168"
     CDS             complement(809..1342)
                     /locus_tag="KPD58_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBG0264951.1"
                     /translation="MSDLNNKKEQDENESSMNETKIYDILDKLNVEYDVVEHEAVYTA
                     EQLVILDGITKGCQCKNLFLRNAKGNKYYLIVVKGEKQVDLNSLKDKIGSSRLSFASP
                     ERLYNVLKLLPGSVNPFSLINDTERKVELYIDKDVIKEDYLNFHPNINTKTVNISRKG
                     FEEFLEYLKYPIQSIDV"
     gene            complement(1386..2018)
                     /locus_tag="KPD58_001169"
     CDS             complement(1386..2018)
                     /locus_tag="KPD58_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HBG0264952.1"
                     /translation="MKVFDLTHVTHNDMPVYAEPNRPDIKKVAIIEENGYQETLISVF
                     SHNGTHMDSPRHMYTKGETLDKLDIENFVGKAYVLELEKGNENIELEYLKKYEDEIKN
                     SDFIIFKSGWSKFWDKKQYYVGYPTLTKEAANYIANTNIKGIGIDMLSVDRYDTSVFE
                     VHHILFEKGKIIIENLTNLENVPEKFLFIAAPFKYNDADGAPVRAIAIVE"
     gene            complement(2108..3187)
                     /locus_tag="KPD58_001170"
     CDS             complement(2108..3187)
                     /locus_tag="KPD58_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HBG0264953.1"
                     /translation="MKIAIFLGAGASAAENLPIQNELFSEYFKKLNPTNYNTKMNREL
                     YIFFKQMFNIDIVKDNIDNANFPTFEEVLGLLDLAEQRKEAFRNFGLENLNNRSDSIR
                     FLRQYLILLMAEAIHNTPRTDNKYHKLLVENLLENDLLLDTTFISANYDIHIDNTVTG
                     LRDKNDLPIMLDYGVEFTNFKEELNWRKPKEPLIKLYKIHGSLNWLYCPICNSVTLTP
                     HEGGVMKLIENSSETKCLECGELTEPIIVPPTYFKNMSNIFLSNVWNETEKTLRDTDL
                     LIFCGYSFPEADMHIKYMLKRVQTNRKKPPLKIMVFNNHSQKQRITLKKEEGRYKRFL
                     GEDVIFTDNSFQDFSVNPLRFIKNI"
     gene            complement(3320..4666)
                     /gene="pepV"
                     /locus_tag="KPD58_001171"
     CDS             complement(3320..4666)
                     /gene="pepV"
                     /locus_tag="KPD58_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HBG0264954.1"
                     /translation="MKEQIKEKVNSLQDEMISSIQESVKIPSVISEATENCPFGENVD
                     KALRGILDLCKSLGFKTVYKDGYYGYAEIGQGEKMIGILGHVDVVPEGDLESWNYPPF
                     EAVLEDGKLYGRGTQDDKGPTISAIYAVKALMDLNVDFNKRIRFIFGADEENLWRCIN
                     KYKENNEEIPNYGFTPDSRFPITNAEKGLLQVHLTCDSKSDIELSVGKALNAVPGKAI
                     YIGKYSDKLKKELDKLNFEYTVEGNKICIIGKSVHSAASDTGINAVARLCIALNNIGI
                     DSNIIKFLAEVIGEDANGNNIIPNCKDDVSGKLTVNIGRVTIDNEKEFAGIDVRIPVT
                     YKKDDFVKELKKMTDKYNLNYEEYDFLDSIYVPEDTLLVKTLRKVYEEETGLDGTPLS
                     SGGATYARALDNCVAFGAIFPGKPETEHQANEYLIVEDIIKATQIYALSIYELLKI"
     gene            complement(4735..6015)
                     /locus_tag="KPD58_001172"
     CDS             complement(4735..6015)
                     /locus_tag="KPD58_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HBG0264955.1"
                     /translation="MLNETKELLKDYYGIDDDTFKLSQEIMEEIKDKFEEIKEIREYN
                     QYKVLKAMQESKLSDMHFNWTTGYGYNDIGREKIEEIYSKVFNTEDALVRPIIVNGTH
                     ALTLCIQGIVRPGDEILSVTGRPYDTLEGVIGIREEKGSLKEYGVTYDDVDFLEDGNL
                     DLEGIKSKINDRTKLVMIQRSKGYSWRKSLSISDIKEAIEVIKSVKPEAIVMVDNCYG
                     EFLDTKEPTDVGADVMAGSLIKNPGGGLALTGGYIAGRKDLIELISYRMTSPGIGKEC
                     GLTFGTTRNVLQGFFLAPYIVSQAVMGAIFCSRAFEKLGYDVLPKYDDLRSDIIQCIR
                     LNNADEVISFCEGIQEAAPVDSYVKPVPWDMPGYESEVIMAAGAFIQGSSIELSADAP
                     IRPPYNVYFQGGLTFDHSKMGTLKAIEFIKKLKK"
     gene            complement(6142..6402)
                     /gene="hfq"
                     /locus_tag="KPD58_001173"
     CDS             complement(6142..6402)
                     /gene="hfq"
                     /locus_tag="KPD58_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HBG0264956.1"
                     /translation="MKNTVLNLQDLFLNNARKERIPVTIYLVNGVQVKGLVKGFDSYI
                     ILIEGDNRQQNMIYKHAVSTIQPGKYINLTNQNQNNNNNNNR"
     gene            complement(6516..7451)
                     /gene="miaA"
                     /locus_tag="KPD58_001174"
     CDS             complement(6516..7451)
                     /gene="miaA"
                     /locus_tag="KPD58_001174"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBG0264957.1"
                     /translation="MKKIPLIILTGPTAVGKTDLSIKLAKDMNAEIISADSMQIYEYM
                     DIGSAKVTEEEMQGVKHYLIDEVKPDYPFSVSEFQQRAKKYIHEINEKEKCVLVTGGT
                     GLYLNSLIYNMDFAQSDANNELREELQKQLAEKGIDYMHNKLKELDEESANRIHKNNT
                     KRVIRALEVCLSGKKMNDFSSDLKFNEEYQPIIIVLNRDREHLYQRINMRVDIMIKNG
                     LVEEAKKLLSMGFKKDMISMQGIGYKEILKYLDGEYTYEEAIEIIKRDSRRYAKRQIT
                     WFKRYKTAKWFDLDQYENIDELKNEIILYIKDSIK"
     gene            complement(7489..9456)
                     /gene="mutL"
                     /locus_tag="KPD58_001175"
     CDS             complement(7489..9456)
                     /gene="mutL"
                     /locus_tag="KPD58_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBG0264958.1"
                     /translation="MKNIINILDDLTINKIAAGEVVERPSSVVKELIENSIDAGANKI
                     SIDIIDGGKSLIKITDNGIGIPSSEVEKSFLRHATSKIKKIDDLYDLYSLGFRGEALA
                     SISAVSKLEMTTKTKDEIIGTKIYVEGGKIISKEPIGSTNGTTIIIKDIFFNTPARQK
                     FLKSTHAETINISDLINKLAIGNPNIQFKYTNNNKQMLNTPGDGKLVNTIRSIYGKEI
                     TENIIDVEFKCNHFKMNGYIGNNNIYRSNKNLQHIYINKRFVKSKIIIDAITESYKSI
                     IPIGKHAVCFLNIEVDPSCIDVNIHPNKLEIKFEKEQEVYIELRDFLKVKLIHSNLIG
                     KYATYSDKKTQPRIAINSREKSTDYKLRNNDLLESTPKNSNITKGKDEVIEVVTLSSE
                     KPINEFQSVSEVLNASVEDDVKNINYLSEDSANDNIQEEFQVDGIKNEGNYYLGDSIK
                     DSEEEYLCSSKRKFSLYGYFVIGVVFNTYIILSKDDSMYLLDQHAAHERILYERYMEK
                     FYRQDINMQILLDPVVIEVSNIDMLQIENNLELFMKFGFELEIFGNNHIMVRCVPTIF
                     GVPETEKFILQIIDNIEEITSNYDLKGERFASMACRSAIKANDKIYDIEIKSLLEQLE
                     KCENPFTCPHGRPIMVEISKTEIEKMFKRIM"
     gene            complement(9467..12310)
                     /gene="mutS"
                     /locus_tag="KPD58_001176"
     CDS             complement(9467..12310)
                     /gene="mutS"
                     /locus_tag="KPD58_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBG0264959.1"
                     /translation="MQIDMNKLTPMMKQYLEVKNRYKDCILFFRLGDFYEMFFEDALV
                     ASKALEIALTGKACGLEERAPMCGVPFHSANSYISKLVENGYKVAIGEQMEDPSTAKG
                     IVRREVIRVITPGTVLDGNLLENKKNNYLLSLYKDGTNIGLTYVDISTGETNATCLNE
                     DKVIEEIAKIHPTEIIINDLDFIEKLRDIATVSNIYINESFSDNYLDINILKEYFPDV
                     YLQKLKFDDKGLIKSSLSILLNYIYNTQKQITSNINNINIYNSSEYMVLDMFTRTNLE
                     LTQTIRGNKKKGSLLHVLDKTSTAMGGRLLRKYVEEPLINKSKIENRLDVIEEIKDDF
                     ILREDLNDILKNIYDIERICGKIAFERVTPKELIHLKNSIEKLPNLKDTINLSNAKIL
                     KEYVSEMDKLDDIYNLIDEAILEEPTITIKDGNIIKSDFSDELKELREISKNGAFLVK
                     EIENREREKTGVKSLKIGFNKVFGYYIEITKANFKQAKLDETYIRKQTLSNAERYITP
                     ELKEIEEKILHAEEKIKSLEYEIFVEIRDTIYKNIDRIQKVAKTIANIDVFVSLATVA
                     HINNYVKPAINENNKLDIRNGRHPVVENIVGEENFVPNDTYLNRGENIINIITGPNMS
                     GKSTYMRQTAIIALMAHIGSFVPAESADIPILDRIFTRVGASDDLSQGQSTFMVEMNE
                     VSLILKNATERSLVILDEIGRGTSTYDGISLAWSIVEYIQKNIRCKTLFATHYHELTD
                     LEEEFKEVKNYSIAVKEDGEGIIFLRKIIPQGADKSYGIYVAKLAKLPDEVIERAKYI
                     LKDLEKNHVYNSVAINGDKENNNIINSNLDEELVKVNQSNFKNKYESLKIEHELIVKD
                     YKHIKKDYDKLNSKFKALNDEVALMKQNDDKEKINQEDSVKEVALTQISFDSVNRDIL
                     SEEILNLDILNMTPLDAINSLYNLQRKAKEIKS"
     gene            complement(12471..13205)
                     /locus_tag="KPD58_001177"
     CDS             complement(12471..13205)
                     /locus_tag="KPD58_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0264960.1"
                     /translation="MLTQMNFYHNKKVKVSIENVSKTYKDIEVLKDISIDVYEGEFVS
                     ILGPSGCGKSTIFNIITKLTDYDSGKVNINGKYSYMYQKDLLLPYKTIIDNVSLPLIL
                     KKEKKSKARSMVKPYFEVFGLSGYEDKYPSELSGGMRQRANFLRTFINSNDIMLLDEP
                     FGALDSLTKTSMQKWLLDVKKKVNSTILLITHDIDEAIMLSNRIYVISKKPSIVKKEF
                     VIDSRKLNEDNLENIIKLKKEIISLL"
     gene            complement(13174..14187)
                     /locus_tag="KPD58_001178"
     CDS             complement(13174..14187)
                     /locus_tag="KPD58_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0264961.1"
                     /translation="MNFKKLGILGLVSVFTLATITGCSSNNNKDKNNDKDTSSKKVTM
                     VLDWTPNTNHTGLFVALDKGYYKEEGLDVEIVQPPESGAETLVATGKADFGISYQEQV
                     TYAKTSEDPLPIKAVATVIQHNTSGFASPKEKNITTAKDFEGKTYGGWGSPSEEAVFK
                     AVMKKNGADFNKLKIVNTGQDDFFAAMKTVDFAWIFEGWDAVKADLIGYDLNFIPVKD
                     LDERLDYYTPLIISNETVLKNNPELAKKFLKATTKGYEYAIKNPEESAKILVKHAPEV
                     DEKLALKSQEYLASKYKDDAPRWGEMKDSVWNNYTSFLKEYKLIDKDMKASDAYTNEF
                     LPQ"
     gene            complement(14209..14979)
                     /locus_tag="KPD58_001179"
     CDS             complement(14209..14979)
                     /locus_tag="KPD58_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0264962.1"
                     /translation="MKNLEIIDKVKDSIYPIATFLFILILWQSMVGVLEVPQYILPTP
                     VDIINVFFKDYQNLSIHAVVTIGEAILGFIVAIIISLVIGILMDFVSIIKKCLYPIML
                     VTQMIPTIIIAPLFMIWFGFGTMPKVLMVMLTCFFPILISFVDGIENIDKDYLNLFKT
                     MDSNKVNTFIHLKFPMAMDKFFSGLKISATYAVMAATVAEWLGGTKGLGVYMLRSKSA
                     YALDKVFASTILVVIFSLMFVGIVQAVKKVVIRHRLID"
     gene            complement(14954..15244)
                     /locus_tag="KPD58_001180"
     CDS             complement(14954..15244)
                     /locus_tag="KPD58_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HBG0264963.1"
                     /translation="MVIADIAVMPLRPYKGEEEMYKVVDACIAEIEKSGLKCEIGAMS
                     TTVEGDFDEVFELLKKVHKIPFNLGCERVITVARVDEKAGGLTIDEKLRNHR"
     regulatory      complement(15375..15474)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     regulatory      16094..16183
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            16236..16412
                     /locus_tag="KPD58_001181"
     CDS             16236..16412
                     /locus_tag="KPD58_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264964.1"
                     /translation="MFMDKMDRCTHILTAYICSSRDYCNFIDTQLNDFILEYGENVVE
                     SCLHQVMVLVSRYN"
     gene            complement(16527..16883)
                     /locus_tag="KPD58_001182"
     CDS             complement(16527..16883)
                     /locus_tag="KPD58_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0264965.1"
                     /translation="MDIDNNFEEFPNIEERQKDFNCSIGFTMTVIGSKWRAIILWHIL
                     KQEPIRYSQLKKEVVHISHKILSQELKYLERDGLIKRISYPTIPPKVEYLSTKRGKSL
                     ESILLELCNWGKQYMR"
     gene            17139..18052
                     /locus_tag="KPD58_001183"
                     /pseudo
     CDS             17139..18052
                     /locus_tag="KPD58_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061260.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
     gene            18283..18894
                     /locus_tag="KPD58_001184"
     CDS             18283..18894
                     /locus_tag="KPD58_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HBG0264966.1"
                     /translation="MINDIKNKFKNYKPYINGYKNMKRASVLIPIVEINNTHYILFEV
                     RSKNLKHQPSEISFPGGKIESGETPYEAVIRETCEELGTFSDNIEVISSLDLLITPVK
                     FIIHPYLGYIKNINNLNINKDEVDHTFLVPIKYLLENPPNTYSNTVKVFPDENLPYGS
                     IPNQKDYKFESGSYPILFYKYKDYVIWGMTAKILENFLNFLSE"
     gene            19192..19530
                     /locus_tag="KPD58_001185"
     CDS             19192..19530
                     /locus_tag="KPD58_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HBG0264967.1"
                     /translation="MFAVGFVVIIALAFFILSVLLFNGKASMLLAGYNSMSKKEKARY
                     DKKKLCRSSSIVTFIVSIMLFIMAFLGYRIESGQMDEKKMVPFSIIFIIVILSSVFFN
                     ILYSNKKCKK"
     gene            complement(19937..21373)
                     /gene="miaB"
                     /locus_tag="KPD58_001186"
     CDS             complement(19937..21373)
                     /gene="miaB"
                     /locus_tag="KPD58_001186"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HBG0264968.1"
                     /translation="MSKRKQVTIPIEKIQEQDKYIEQIHRQNEEYFNRTGKRKLVFTQ
                     TFGCQMNEHDSEKLCSMLEEMGYQMSMMVEESDLIIYNTCAVRENAELKVYGNLGQLK
                     HLKGKNPDMKIAVCGCMMQQPHVVEELRKKYKHVDLIFGTHNLYKFPQLLTESINSDK
                     MLVDVWDVDGEVIEGLRSNRKFELKAFVNIMYGCNNFCTYCIVPYTRGRERSRTPEDI
                     INEIKELVANGTKEITLLGQNVDSYGKTLENPVSFSELLRKVNDIEGIERVRFMTSHP
                     KDISDEVIYAIRDCDKVCEFLHLPIQCGSSSLLKKMNRHYTKEYYLEIIEKAKKEVPG
                     IAFSTDLMIGFPGETEEDLLDTLDVVEKVRYDSAFTFIYSKRQGTPAAKMENQIPEDI
                     KHDRFNRVLEAVNRISAEINDGYKDRIVEVLVEGRSKNNENKFAGRTRQNKLVNFEGG
                     NDDLIGKLVMVKITEPRTFSLNGILVNN"
     gene            complement(21621..23093)
                     /locus_tag="KPD58_001187"
     CDS             complement(21621..23093)
                     /locus_tag="KPD58_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp28"
                     /protein_id="HBG0264969.1"
                     /translation="MKFYKRILTLTLSISFVFANIQLVDAISSVEKIQGKNKYEIAGK
                     IADKNAYKTAYKTAYKTAYKTAILINTSNSIADGLSASGLAGALNAPILLTEKNTIPT
                     ETSARLKNVSKVYIIGGTYSISTSVENSLKSKKMKVVRIKGNDRIKTSYNVAKEINSI
                     KKVNTVMLTNAYKGEADAISIASVAARDKAPIILTNGQSIPFSTSGLKSYVIGGTASM
                     STTLVNNTKSTRLGGSTRFETNKAIINKFYKDAREFYIAGAYELTNALVGSSLSKHGP
                     MVLVNDGSNKSILKNAKKITSIGYIDSNIVQQCLNITNGIGDINTGVVKNVKPTTKTI
                     KDGMYKVGKDISAGEYLITSNSGSYASYYEVTSDSTGNADSILSNDIFSGTRYITLKN
                     GQYIKIEDSTMTLAKYAKAQKAKNGKFGNGMYKIGLEIPAGEYIIMSNSSDAYYEVRN
                     DSLGNAEGIVTNDTFSGRRYITVEEDQYLILNDCYLIENE"
BASE COUNT         7511 a         3821 c         2485 g         9327 t
ORIGIN      
        1 tctaaataaa aaatttgtct aaagtttcta aaaaatttag acaaatctta ggttattact
       61 ctttaaatta tttttaaatt attccaaata cgccaaataa tattggtata aatatagctg
      121 gtaacaaatt ggctactttt atattcgtta atttaagtac atttagacca agtcctataa
      181 ttaataaact tccagctgct gtcatttctg tgactacagg atcacttaaa aatccattta
      241 aaaatgatgc tcccacacat ataatacctt catacaaaaa tacagatatt gcagaaaaca
      301 ttacacctat acctaaagaa gcagtaaata taattgaagc tattccatct ataacagatt
      361 ttacaaataa agtatcatga cttcctgtaa gaccactttc taatgaacca actactgcca
      421 tggctcctat acagaataat aaactagatg atataaaacc ctctgctata gaatcttttt
      481 tatttccttt agaaaattta ctttgtaaaa aagcacccaa cttatttacc catttgtcaa
      541 tatctattaa ttctcctatc agagcaccta cagctacgga tattattgtc actaatgtgt
      601 tttttccttc tagtgctcct gatataccaa tgtacaatac acataaagca attccattca
      661 ttatagtatc gcttactctt tgaggtaatc ctccttttat tataagacct actatacatc
      721 cacctgcaat tgctagacta tttgctatta ctccaaacat tttttctccc ctcgattagc
      781 taattgaaat ttatcctatt attaaaacct aaacatctat actttgtatt ggatatttta
      841 aatattccaa aaattcttca aaaccttttc tagatatatt tacagtttta gtatttatgt
      901 taggatgaaa atttaagtaa tcttctttaa ttacatcttt atctatgtaa agctcaactt
      961 ttctttctgt atcatttatt aatgaaaaag gatttacact tccaggaaga agtttaagta
     1021 cattgtatag tctttcaggt gaagcaaacg ataatctaga acttcctatc ttatctttaa
     1081 gtgagtttaa atcaacttgt ttttctcctt taactacaat aagataatac ttatttcctt
     1141 tggcatttct taaaaataaa tttttacatt gacacccctt tgttattcca tctaaaatga
     1201 ctaattgctc tgctgtatat acagcttcat gctctactac atcatattca acatttaatt
     1261 tatccaaaat atcatatatt ttagtttcat tcatgctaga ttcattttca tcttgttctt
     1321 ttttattatt taaatcactc atacttttct ccttaaacac ttcatcataa tatcattgtg
     1381 aggtattatt ctactatagc tattgctctt actggagctc catcagcatc attatactta
     1441 aatggtgctg ctataaatag aaatttttct ggaacatttt ctaaatttgt aagattttct
     1501 atgattattt ttcctttttc aaataaaata tgatgcactt caaatactga tgtgtcatat
     1561 ctatccactg atagcatatc tattcctatg ccttttatat tagtattagc tatatagttt
     1621 gcagcttctt ttgttaatgt aggataacct acatagtact gctttttatc ccaaaatttt
     1681 gaccaacctg atttaaatat tataaaatca ctatttttta tttcatcttc atattttttt
     1741 agatattcta attctatatt ctcatttccc ttttcaagtt ccaatacata agctttccca
     1801 acaaaatttt ctatatccaa cttatctagg gtctcacctt tagtatacat atgtcttggt
     1861 gaatccatat gagttccatt gtgagaaaat acactaatta atgtttcttg ataaccattt
     1921 tcttcaataa tagctacttt cttgatatct ggtctattag gctctgcata tacaggcatg
     1981 tcattatgtg tcacatgagt taaatcaaaa accttcatct ttatataccc cctatgtttt
     2041 tattttctat tatataccta aattcatttt atttctatca gattttattc cattatatac
     2101 catactacta tatatttttt ataaacctca agggatttac cgaaaaatct tggaaagaat
     2161 tatcagtaaa tataacatct tcacccaaaa aacgtttata ccttccttct tctttcttta
     2221 aagtaattct ctgtttttgt gaatgattat taaaaaccat tatcttaaga ggaggttttt
     2281 ttctattagt ctgcactctt tttaacatgt attttatgtg catatcagct tcaggaaatg
     2341 aatatccaca aaatattaaa aggtcagtat ctcttaaagt tttttctgtt tcattccaaa
     2401 cattacttaa aaatatgttt gacatattct taaaatatgt tggtggcact ataataggtt
     2461 ctgttaactc accacactct aaacattttg tctctgaact attttcaata agcttcatga
     2521 caccaccttc atgtggtgta agagttacac tattacaaat ggggcaataa agccaattca
     2581 atgacccatg tatcttatat aatttgatta atggttcttt tggttttctc caatttaact
     2641 cctctttaaa atttgtaaac tccacaccat aatcaagcat aattggcaaa tcattcttat
     2701 ctcttaatcc agtaactgta ttgtctatat gtatatcata atttgcacta ataaaagtag
     2761 tatcaagaag caaatcattt tccaaaagat tttcaactaa aagtttatga tatttattat
     2821 ctgttctagg tgtattatga attgcttcag ccattagcag tattaaatat tgtcttaaaa
     2881 aacgtatact atcacttctg ttatttagat tttctagtcc aaaatttcta aatgcttctt
     2941 ttctttgttc tgctaagtct aaaagaccta aaacttcttc aaaggtagga aaatttgcat
     3001 tatctatatt atcttttact atatcaatat tgaacatctg tttaaagaaa atatacaatt
     3061 ctctattcat tttggtatta taatttgtgg gatttagttt tttaaaatat tcgctaaaaa
     3121 gttcattttg aataggtaga ttctctgctg ctgaagctcc tgctcctaaa aatattgcta
     3181 ttttcatata gttcctccta atataaatat tactcctatt tttatatcca ttaaatatcc
     3241 atatcattta ttttatgata caacaataaa gctgtctttt actagaaaaa atcctattac
     3301 aagacagcct tatattatat taaatcttta ataattcgta aatagaaaga gcatatattt
     3361 gagtagcttt tataatatcc tctactatta agtattcatt agcttggtgt tctgtttctg
     3421 gttttcctgg aaaaatagct ccaaatgcaa cacaattatc taaagctctt gcgtatgttg
     3481 ctcctccaga tgaaagtggt gttccatcaa gaccagtttc ttcttcatat acttttctaa
     3541 gtgtcttaac aagaagagta tcttctggaa catagattga atccaagaaa tcatattctt
     3601 cataatttaa attgtattta tctgtcattt tctttaactc ttttacaaaa tcatcctttt
     3661 tgtatgttac tggaatcctt acatctattc ctgcaaattc tttttcgtta tcaatagtta
     3721 ctctacctat atttactgtt agtttacctg atacatcatc cttgcaatta ggaattatat
     3781 tgtttccatt agcatcttca ccaataactt ctgctaaaaa cttaattata ttagaatcta
     3841 tacctatatt attaagagct atacaaagtc tagccactgc atttatacca gtatcacttg
     3901 ctgctgaatg gacacttttt ccaattatac agattttatt accttcaaca gtatattcaa
     3961 aatttaattt atcaagttct ttttttaatt tatcagagta ttttccaata tagattgctt
     4021 tacctggaac tgcatttaaa gcctttccaa ctgacaactc tatatcactt ttgctatcac
     4081 aagtcaaatg aacttgtaaa agacctttct ctgcatttgt tattgggaat ctagaatctg
     4141 gagtaaatcc atagtttggt atttcttcat tattttcttt atatttattt atacatctcc
     4201 aaagattttc ttcatcagcc ccaaaaatga atcttatcct cttattaaag tctacattta
     4261 aatccataag agctttcact gcataaattg ctgatattgt aggtccttta tcatcttgtg
     4321 ttcctcttcc atacaattta ccatcttcta aaacagcttc aaatggaggg taattccagc
     4381 tttctaaatc accttctgga accacatcaa cgtgaccaag tatacctatc attttttcac
     4441 cttgtcctat ttctgcatat ccataatatc catctttgta tactgtctta aatcctaaag
     4501 atttacataa gtctaatata cctcttaaag ctttatctac attttcacca aatgggcaat
     4561 tttcagttgc ttcagatata acactaggta tttttacact ttcttgtatt gaagaaatca
     4621 tttcatcttg tagagaattt actttttctt taatttgctc tttcatatta tcacccttca
     4681 attttcatca taatatacta ataaatattt tgacctttta aaaaaaaaat atgcttattt
     4741 tttcagtttt tttataaatt caatcgcttt taaagtcccc atttttgaat ggtcaaatgt
     4801 aagtccacct tggaaataaa cattataagg aggtcttatt ggagcatctg cacttaattc
     4861 tatagaagaa ccttgtataa atgctcctgc tgccattatg acttcacttt cgtatccagg
     4921 catgtcccaa ggaacaggtt ttacataaga atcgactggt gcagcttctt gtataccttc
     4981 acaaaaactt attacttcat cagcattatt aagtctaata cattgaataa tatcacttct
     5041 taagtcatca tattttggta atacatcata tcctaatttt tcaaaagctc ttgagcaaaa
     5101 gatagctccc attacagcct gagatactat ataaggcgcc aagaaaaaac cctgaagtac
     5161 atttctagtt gttccaaaag taagaccaca ttcttttcct attccaggag aagtcattct
     5221 ataagatata agctcaatca aatcttttct accagctata tatccacctg tcaatgcaag
     5281 cccaccacca ggatttttta ttaaagagcc agccataaca tctgctccaa catcagtagg
     5341 ctctttggta tctaaaaact caccataaca attatcaacc attactatag cttctggttt
     5401 aacagacttt attacttcta tagcttcttt tatatcacta attgaaagtg attttctcca
     5461 agaatatcct tttgaccttt gtatcataac gagtttagtt ctatcattta ttttgctttt
     5521 aattccttct aaatctaagt ttccatcctc taaaaaatct acatcatcat atgtaacacc
     5581 atactctttc aatgaacctt tttcttctct tatgcctata actccctcta aagtatcata
     5641 tggtctacca gtaacagata aaatttcatc tcctggtcta actatacctt gtatacataa
     5701 agtaagtgca tgtgtaccat ttacaattat tggtcttaca agagcatctt ctgtattaaa
     5761 tactttagaa taaatttcct ctattttttc acgaccaata tcattatatc catatcctgt
     5821 agtccagtta aagtgcatat cacttagttt agattcttgc atagctttta aaactttata
     5881 ttgattatat tctcttattt cttttatttc ttcaaactta tcttttattt cttccattat
     5941 ttcttgtgat agtttaaaag tatcatcatc tattccatag tagtctttta ataattcttt
     6001 tgtctcatta agcattttat cacctgtctc tttattattt tatttaaaca tgtaaaggag
     6061 actattttac taaaacaaat aaattatagt ttataagtta gtataagtct cctctcaatc
     6121 atcttaaatt aaattaatta tctatctgtt gttattatta ttgttgtttt ggttttgatt
     6181 tgttagatta atatactttc caggttgtat agttgataca gcatgtttat aaatcatgtt
     6241 ttgttgtcta ttatcccctt ctattaatat tatataacta tcaaacccct ttacaagccc
     6301 tttaacttgc actccattaa ctaaatatat agtaacaggt atcctttctt tccttgcatt
     6361 atttaaaaac aaatcttgta aatttaaaac tgtatttttc attagatttg taccctccca
     6421 agattaaatt gaacttctaa aatattccta ccttatattc tatataagtt atgacaaatt
     6481 ttcaatatta agttgtatac ttaaaaattt atatattatt ttatagaatc tttaatatat
     6541 aagattattt cattttttaa ttcgtctata ttttcatatt ggtctaaatc aaaccatttt
     6601 gctgttttat atcttttaaa ccatgttatc tgacgttttg cataccttct agaatctctt
     6661 ttaattattt caatagcttc ttcgtatgta tattctccat ccagatactt taatatctct
     6721 ttataaccta taccttgcat tgatatcata tcttttttaa atcccatact taaaagtttt
     6781 tttgcctctt caaccaatcc atttttaatc ataatatcaa ctctcatatt tattctttga
     6841 tacaaatgtt ctctatctct atttagaact attattattg gttgatattc ttcattaaat
     6901 tttaaatcac ttgaaaaatc attcattttc ttaccactta gacaaacttc aagagctctt
     6961 atgacccttt tagtattgtt tttatggatt ctattagcag attcttcatc aagttctttt
     7021 aacttattat gcatgtaatc tattcccttt tcagcaagct gtttttgaag ttcttctctt
     7081 aattcattat tggcatcaga ttgtgcaaaa tccatattat atattaaaga gtttaaataa
     7141 agtcctgtgc ctccagttac taaaacacat ttttcctttt catttatttc atgtatatat
     7201 ttttttgctc tttgttgaaa ctctgaaact gaaaatggat agtcaggctt aacttcatct
     7261 ataaggtaat gcttcactcc ttgcatctct tcctctgtaa cttttgcact tccaatatcc
     7321 atatattcat atatttgcat agaatcagct gaaattatct ctgcattcat atcttttgct
     7381 aattttattg acaaatctgt ttttccaaca gcagttggac ctgtaagtat tataagtggt
     7441 atttttttca taacattcct ttccatatat tttaaatatc caaatatttt acataattct
     7501 tttaaacatt ttttctattt ctgtcttaga tatttcgacc attataggtc ttccatgagg
     7561 acatgtaaat ggattctcac acttttctag ctgttccaat aaacttttta tttcaatatc
     7621 gtatatttta tcatttgctt ttatagcaga cctacatgcc atagatgcaa atctctctcc
     7681 ttttaaatca taattacttg taatttcttc tatattatca attatttgaa gtataaattt
     7741 ctcagtttca ggtactccaa atatagtagg aacacatctt accattatat gattattgcc
     7801 aaaaatttct agctcaaaac caaatttcat aaataactcc aagttatttt caatttgaag
     7861 catatctata tttgatactt caataaccac tgggtctaaa agtatttgca tatttatatc
     7921 ttgtctatag aacttctcca tatatctttc atatagtatt ctttcatgtg cagcatgttg
     7981 gtccaacaaa tacatagaat cgtctttgct caaaattata taagtattaa atacaactcc
     8041 aattacaaaa tatccgtata aagaaaactt gcgttttgaa ctacacaagt attcttcttc
     8101 actgtcctta atactatctc ctaagtaata atttccttca tttttaattc catcaacttg
     8161 aaactcttct tgtatattat catttgcact atcttcactt agataattaa tgttttttac
     8221 atcatcttct acacttgcat ttaggacttc acttactgat tgaaattcat ttattggttt
     8281 ttcacttgat aaagtaacaa cttcaatgac ttcatcctta cctttagtaa tattactatt
     8341 tttaggagta gattctaata aatcattatt tcttaattta taatctgtac ttttttctct
     8401 actgttaata gcgattctag gttgtgtttt tttatcacta taagtagcat attttccaat
     8461 aagatttgag tgtattaatt taacctttaa aaaatctcta agttctatat acacttcttg
     8521 ttctttttca aatttaattt caagtttatt ggggtgtata tttacatcta tacatgatgg
     8581 gtctacttct atatttaaga agcacactgc atgtttacct attggaatta tagacttata
     8641 actttctgtt attgcatcta tgattatttt actttttaca aatcttttat ttatatagat
     8701 atgttgtaag tttttatttg aacggtatat attgttatta cctatatatc cattcatttt
     8761 aaagtgattg cacttaaact ctacatctat tatattttcc gttatttctt ttccatatat
     8821 acttctaata gtgtttacaa gttttccatc ccctggagta tttagcatct gtttattgtt
     8881 atttgtatat ttaaattgaa tgtttggatt acctattgca agtttattta tcaaatcact
     8941 tatgtttata gtttctgcat gagtagattt taaaaatttc tgtcttgctg gtgtattaaa
     9001 gaaaatatct tttataatga tggtagttcc attagtagaa cctattggtt ctttcgatat
     9061 tatttttcca ccttcaacat atattttagt tcctattatt tcatctttag ttttagtcgt
     9121 catttctaat tttgatacag ctgaaataga tgccaatgct tctcctctaa aaccaagaga
     9181 gtataaatca tacaaatcat caattttttt aattttacta gtagcatgtc tcaaaaatga
     9241 tttttcaact tcacttgaag gtattcctat tccattatct gtaatcttaa ttagactttt
     9301 tcctccatca attatatcta tggaaatttt atttgcacct gcatcaatgg agttttcaat
     9361 aagttctttt actacagaag aaggtctttc tacaacttca cctgctgcta tcttatttat
     9421 agtcaaatca tctaggatgt taattatatt tttcattttt acacctctaa gattttattt
     9481 cttttgcttt tctttgaaga ttataaagag agtttatagc atctaatgga gtcatattta
     9541 aaatatccag atttaaaatt tcttctgata gtatatctct atttacacta tcaaaagaga
     9601 tttgagttaa ggctacttct tttacagaat cttcttgatt aattttctct ttatcatcat
     9661 tttgcttcat taaagcaact tcatcattta atgctttaaa tttagaattt aatttatcat
     9721 aatccttttt tatatgtttg tagtctttta ctataagttc atgttctatt tttagagatt
     9781 catatttatt cttaaaatta ctttgattta ccttaactaa ttcttcgtcc aaattactat
     9841 taattatatt attgttttct ttatctccat ttattgcaac actgttgtaa acatgatttt
     9901 tttccaagtc tttaagtata tattttgctc tttctatgac ttcgtctggt aatttagcta
     9961 atttagcaac atatattcca taactcttgt ctgctccctg aggaattatt tttcttaaaa
    10021 atattattcc ttccccatct tctttgactg ctattgaata atttttaacc tctttaaact
    10081 cttcttctaa atcagtaagt tcatgataat gtgttgcaaa taatgtctta catctaatat
    10141 ttttttgaat atattccact attgaccatg ctaaacttat accatcatag gtactagttc
    10201 cacgacctat ttcatctaat ataactaaac ttctctctgt tgcatttttt aatataaggg
    10261 atacttcgtt catctctacc ataaaagtag attgaccttg agataaatcg tcactagctc
    10321 caactcttgt aaatattcta tctaatattg gaatatcagc agactctgct ggtacaaatg
    10381 agcctatatg tgccataaga gctattatag cagtttgcct catatatgtt gatttacctg
    10441 acatattagg accagttatt atgttaataa tgttttctcc acgatttaaa taagtatcat
    10501 ttggaacgaa attttcctct cctactatat tttcgacaac aggatgtcta ccatttctta
    10561 tatcaagttt attattttca tttattgctg gttttacgta gttatttata tgtgcaactg
    10621 ttgcaaggga cacaaaaaca tctatattag ctatagtctt agccactttt tgtattctat
    10681 ctatgttttt atatattgta tctcttatct ctacaaatat ctcgtattct aaagatttaa
    10741 tcttttcctc tgcatgtagt atcttttctt ctatttcctt taattctgga gttatatatc
    10801 tttctgcatt actaagtgtt tgctttctta tatatgtctc atctagcttt gcctgtttaa
    10861 agttggcttt tgttatttct atataatacc caaaaacttt attaaatcct atttttaatg
    10921 atttaactcc tgttttttct ctttccctgt tttctatttc ctttacaaga aaggctccat
    10981 tcttagaaat ctctcttagt tccttaagtt catcactaaa atctgattta attatgtttc
    11041 catcttttat ggttatcgta ggttcttcta gaatagcttc atctattaaa ttgtatatat
    11101 catctaattt atccatttct gaaacatact cttttaggat ttttgcattt gataaattta
    11161 tagtatcttt taaattaggt aacttttcta tcgaattttt taagtgaatt aactcttttg
    11221 gtgttactct ctcaaatgct atctttccac atattctttc tatatcgtat atatttttaa
    11281 gtatatcatt taggtcttct cttaatataa agtcatcttt tatttcttct attacatcta
    11341 atctattttc aattttagat ttatttatca aaggttcttc tacatatttt cttaaaagtc
    11401 tacctcccat tgctgtagaa gttttgtcaa gtacatgtaa taatgaacct tttttcttat
    11461 tccctcttat agtttgagta agttctaaat tagttctagt aaacatatct agaaccatat
    11521 attctgatga attgtatata tttatattat taatgttaga tgttatttgt ttttgagtat
    11581 tgtatatata attcagaaga attgataagc ttgactttat tagaccttta tcatcaaact
    11641 ttagtttttg tagataaaca tctggaaaat attcttttaa tatatttata tccaaatagt
    11701 tatcactaaa actttcattt atgtatatat tacttacagt agctatatct ctaagctttt
    11761 ctataaagtc taaatcattt ataattattt cagttggatg gatttttgca atttcttcaa
    11821 ttaccttatc ttcatttaaa catgttgcat tagtttctcc tgtacttata tcaacataag
    11881 ttagacctat attagtacca tccttataca aagataataa ataattattc tttttatttt
    11941 caagtaaatt tccatctaaa actgtaccag gagtgatgac ccttataacc tctcttctaa
    12001 caattccttt tgctgtagat gggtcttcca tttgttcacc tattgctact ttatatccat
    12061 tttcaactaa ttttgatata tatgaattgg ctgagtggaa aggaacacca cacataggag
    12121 cacgttcttc taaaccacaa gcttttcctg tcaatgctat ttcaagagct tttgaagcta
    12181 ctaatgcatc ttcaaaaaac atttcataaa aatctccaag tctaaaaaac agtatacagt
    12241 ctttatatct attttttact tcaagatact gtttcatcat aggagttaat ttattcatat
    12301 ctatttgcat tatcaaacct ccttcttttc taattaaata ttatatcata gtagatgatt
    12361 attattaaca caatatataa aattttgaca tataactaaa aacaatccaa aatgtatact
    12421 cacattttgg attgctaagt ttaatgctta ttaaataaaa atattttttt ttacaataaa
    12481 gatattattt cttttttaag tttaatgata ttttcaaggt tatcttcatt taactttcta
    12541 ctatctataa caaattcttt ctttactatt gatggttttt ttgaaataac ataaattctg
    12601 tttgatagca ttatagcttc gtcaatatca tgagttataa gaagtattgt tgaatttact
    12661 tttttcttaa catctaaaag ccatttttgc atggatgttt tagttaatga atcaagtgcc
    12721 ccaaaaggct catctaaaag cattatgtca ttagaattta taaaagttct caagaagtta
    12781 gctctctgtc tcattcctcc agaaagttct gaaggatatt tatcttcata tccgcttaat
    12841 ccaaacacct caaaataagg ttttaccatt gacctagctt tactcttttt ttcttttttt
    12901 aaaattagtg gtagtgacac attatctatt atagttttat atggcaagag taaatctttt
    12961 tgatacatat aactatactt accatttata ttcactttgc cactatcata atctgtaagt
    13021 ttagttataa tattaaaaat agtacttttt ccacatccac ttggccctaa tatagaaaca
    13081 aactcacctt cataaacatc aatacttata tcttttaaaa cttcaatatc tttataagtt
    13141 tttgatacat tttctataga tacttttact tttttattgt ggtaaaaatt catttgtgta
    13201 agcatcactt gccttcatat ccttatctat taatttatat tcttttaaga atgatgtata
    13261 gttgttccat acactatctt tcatttctcc ccatcgtggt gcatcatctt tgtacttgct
    13321 tgctaaatat tcttgactct ttaaagctag tttttcatct acttcaggtg catgttttac
    13381 taaaattttt gctgattctt ctggattttt tattgcatat tcataacctt ttgttgtagc
    13441 ttttaagaat tttttagcaa gctctggatt atttttaagc acagtttcat tagaaattat
    13501 aagtggtgta taataatcca atctctcatc taaatctttt actggtataa agtttaagtc
    13561 atatccaatt aaatcagctt taactgcatc ccaaccttca aatatccatg caaaatctac
    13621 agttttcata gctgcaaaga aatcatcttg accagtatta actatcttta acttattaaa
    13681 atcagctcca tttttcttca taactgcttt aaaaacagct tcttctgatg gagagcccca
    13741 accaccataa gtttttcctt caaaatcttt agctgttgtt atattctttt cttttggtga
    13801 tgcaaaccca gaagtattat gttgtataac agttgctaca gcttttattg gaagtggatc
    13861 ttcactagtc tttgcataag taacttgttc ttgatagctt ataccaaagt cagcttttcc
    13921 tgttgctact aaagtttctg ctccactttc aggtggttgt actatttcaa catccaaacc
    13981 ttcttcttta tagtaaccct tatcaagtgc tacaaataat ccagtatgat ttgtatttgg
    14041 tgtccaatca agcaccatag ttactttttt agaagaagta tctttatcat tgtttttgtc
    14101 tttattgtta tttgaagaac atccagttat tgttgccaat gtaaaaacac ttactaaacc
    14161 taatattcct aactttttaa aattcataat tgcctccctc aaataccctt aatctattaa
    14221 tctatgtctt ataacaactt tttttacagc ctgtacaatt cctacaaaca ttaaactaaa
    14281 tataactact aatatagtag atgcaaatac tttatcaagt gcatatgcac tttttgacct
    14341 aagcatataa acaccaagtc ctttcgttcc acctagccat tctgcaacag ttgctgccat
    14401 tacagcatac gttgcagata ttttaagtcc agaaaaaaat ttatccattg ccataggaaa
    14461 tttaaggtga atgaatgtat taactttatt tgagtccata gtcttaaata aatttaaata
    14521 atctttatca atattctcaa tgccatctac aaaacttatt aatataggaa aaaaacaagt
    14581 aagcattacc atcaatactt ttggcattgt tccaaatcca aaccatatca taaagagtgg
    14641 tgctataatt attgtaggta tcatctgtgt aaccaacata ataggataaa gacatttttt
    14701 tattattgat acaaaatcca tcaatattcc aattaccaat gatattataa ttgcgactat
    14761 aaaaccaagt attgcttctc ctatagtaac cacagcatgt atggataaat tttgataatc
    14821 tttaaaaaac acattaatta tatctactgg tgtgggtagt atatactgag gaacttctaa
    14881 aactcctacc atagattgcc aaagaataag tataaacaaa aatgttgcta ttggatatat
    14941 actatctttg actttatcta tgatttctaa gtttttcatc tatagtcaga cctccagctt
    15001 tttcatctac tctagctact gttatgactc tttcacatcc aagattaaaa gggattttat
    15061 gtaccttttt taataattca aaaacttcat caaaatcacc ttcaacagta gtgctcattg
    15121 ctcctatttc acatttaaga ccactttttt caatttcagc aatacaagca tctactactt
    15181 tatacatctc ttcttcacct ttgtaaggtc ttagtggcat tactgctatg tctgcgatta
    15241 ccatttaaat cactccttta aaaataatta atacctacta ggtatatttt gttttttatt
    15301 aaaaaatttt attactaaaa agatattata tttaaataaa agaaaagtct tatccaaaat
    15361 aggataagac ttatttttat cttaactggt ctccctacac tggcattatc caaacaggtt
    15421 caaagggtcg agataaaatc tcctctcagc acattagctc cccgaaatat attatatctt
    15481 ttctacttga ttatactaca cttatcatat tttaacaaat tttttattta tattagatat
    15541 tttatccgtt caaaaatatc taatataaat tgtacattat tacaccaaat aaattatctt
    15601 aaaatggtaa tttatcaaca tcttccaaaa ctagattaac tgaattttct agtttttatt
    15661 ttgctatttc aagcatattt ggatgtatat caattccata caaatattgt gctaaatttc
    15721 tgttttcact catctaattt gcaatcgtta caaattacag attagaaata gcttttagac
    15781 tctacttatc tttatctaca aaaaatcctt caataaattc attaataata ttcagtaatc
    15841 taaaaataaa cctctatcta gatttttaag atacaagcct tttaggaagt atcttttgtg
    15901 ataatataaa aattactcat cattatcatc aaaatcctca gcaaccaaca ttttagataa
    15961 gaatcaaact ctataatata attattttat ccaatttatt tttgagaaat aaaatatttt
    16021 tttaattttt aaatttttta atatgcacct tgaaaaaaga aacgttttcc tatattatgg
    16081 taatagtaga ataaaactaa ataggcaaaa ctagagaaat ttagtgacgc aaagctatag
    16141 ggactaagac ttatgaaatg agttatgtca gccagttgcc aaagagatat tttctttttg
    16201 tttttatgaa tgttttttag ggggatatat gatatatgtt tatggataaa atggatagat
    16261 gtacacatat actaactgca tatatatgca gttcacgcga ttactgcaat tttatagata
    16321 cacaattaaa tgattttata ctagaatatg gagagaatgt ggtagaatct tgtttgcatc
    16381 aagtgatggt attggtaagc agatataatt aaagaaattt tattttctag aaaacccttt
    16441 ttagttttat ctacgtttta taattcatta aagaactaat taaaataagc ttcattatat
    16501 atttttcata aagtaaacag ttgagtttat ctcatatact gtttacccca attacataat
    16561 tctaataata tagattctaa tgatttacca cgtttagtag acaaatattc aacttttgga
    16621 ggaatagtcg gataagaaat cctcttaata agtccatctc tttcaagata ctttaattct
    16681 tgtgacagta tcttatgact tatatgaaca acttcttttt ttaattgact gtaacgtatt
    16741 ggctcttgtt ttaaaatatg ccataaaata attgccctcc atttacttcc tatcacagtc
    16801 atggtaaaac caattgaaca attaaaatct ttttgtcttt cttcaatatt tggaaattct
    16861 tcaaaattat tatctatatc catctcaatc ctctctttca aaatagtaag tatctaacta
    16921 aaaagtgcat tcttgtttag cgtactaatt cgtaatataa tcttgattgt agtatagagc
    16981 cttacataat actacttttt tagatgtata tgtttaaact ttaatataga catatgttat
    17041 tttattaatt ttatttaaac agataataat aaataataat ttatctcaag ttttctatgt
    17101 agataaacta atctaatgtt tagaaaggat tatataatat gaataaaaaa tttcgtcttt
    17161 atcaagttga ctcattacaa aagaaaaatt tacaggtaat ccagctggtg taattacaaa
    17221 tgctgatggc ttatctgaat cacaaatgca aaaaatagca agagaattaa ataattcaga
    17281 aactgctttt attttcaata acttggctaa aaatcatcaa gaaaattatg atgtacatat
    17341 cagatttttt acactaacta atgaggttcc tatatgtggt catgctacta ttgctgccca
    17401 ttatgttaga gcagtagaaa accaattcaa taacaaaaca gtatttcaaa aaactagtgc
    17461 tggaatatta ccagttgata ttataaaaaa agataatgat tatgaaataa ttatgacaca
    17521 aggaaaaatt gaagtatctc caccgttaca acaaacctat caaaaaagat tacttaatgc
    17581 atttggtatt ttaaaggaag atattcgtac agattgtcct attgccatat catctacagg
    17641 acattcaaaa gttatgattg gaataaagga attatcaaca ttaaatagtt tatctccaaa
    17701 tttatatgaa ttatcgaaaa taagtcaaga aataaaatgt aatggatatt atatttttac
    17761 attacataat aaagaaactc tacaagttca tggaagaatg tttgctcctg caattggtat
    17821 ttcagaagac cctgttacag gaaatgctaa tggaccctta ggtgcgtacc ttgtacatta
    17881 taaagtaata cctacaccca acacagagtt tatatttgaa gcagttcaag gagaaacaat
    17941 aaaacgcaaa ggcaagatgt tagtaaaagt aattatttta gataataaac cagttgaagt
    18001 aaaaataatt ggaaaagctg taattgcatt ctcaacaaac atagaaatct aattatagat
    18061 tccttctaag taaatttttg aactttttaa tactaattta ataacaaact aatttatata
    18121 caatcaaatt aattttttta ttatttgtta atatatattt atcattatat tatataataa
    18181 tatagatagt tactatatct tataattaga taaatcataa ttatatatta ttgttaaaac
    18241 tcaattatat aatttttaat aatattattt taggaggtaa aaatgataaa cgatattaaa
    18301 aataaattca aaaattacaa accatatata aatggataca agaatatgaa gagagcatct
    18361 gttttaattc caatagtaga aataaataac acacactata tattatttga agttcgctca
    18421 aaaaatttga agcatcaacc tagtgaaatt tcttttccag gtggtaaaat agaatcaggt
    18481 gagactccat atgaagctgt tattagagaa acttgtgagg aacttggaac ttttagtgac
    18541 aatatagaag taatatcaag tcttgacctc cttataacac cagtaaagtt tatcatacat
    18601 ccatacttag gatatataaa aaatattaat aatttaaata taaacaagga tgaagttgac
    18661 catacttttt tggttcctat aaaatatctt ttagagaatc ctccaaatac ttatagtaat
    18721 actgtaaaag tttttccaga cgaaaatcta ccatatggtt caattccaaa ccaaaaagat
    18781 tataaatttg aaagtggaag ttatcctatt ctgttttata aatataaaga ttatgtaatt
    18841 tggggaatga cagcaaaaat actagaaaac tttttaaatt ttttatcaga ataacactca
    18901 taataaatca tcaaaacttt tacataagaa atttataaaa gtttctaaat ttaacaattc
    18961 taaaatatca ataactatat tttgtagcac ttttttattt tattattgtt tgaatatttg
    19021 atggttttca atgcaatttc aacataaata ctatgtatta gtgtatactg ttgatgaatt
    19081 aaagtatgat aatctattta ataaagtatg aagtaaatga ttttaatgtg cctttatggt
    19141 aaaattacta ataagtaata aaattaatta aaatatatgg aggtgcttat aatgtttgct
    19201 gtaggatttg ttgttataat tgcacttgcc tttttcattt taagtgtact attatttaat
    19261 ggaaaagcca gtatgctact agcaggttat aactctatga gtaagaaaga aaaagcacga
    19321 tacgataaaa aaaaattatg tagaagttct agcattgtta cttttattgt ctctataatg
    19381 ctatttatta tggcgttttt gggatataga atagagagtg gtcaaatgga cgaaaaaaaa
    19441 atggttccct tttctataat atttataata gtcatacttt ccagtgtatt ttttaatatc
    19501 ctctactcta acaaaaaatg taaaaaataa atagttgtaa gttgagcagt taattataaa
    19561 agattgactg ctttttcatg tatattgact actcttatta aatattttaa catttaaaaa
    19621 tctatgtaaa aataatgaat atagtacaaa cttctaataa tgattttacc tttgtggtta
    19681 cagtccacta tatataattt aatatgataa cagggaatta ctaatgacaa ctaggtaagc
    19741 ttttataaaa tataataaaa caaaaataga tgtacaatga aattatatct tatatcaaat
    19801 ttatttttat taatagcaaa aacttacatt agcataacat atgacaaatt cgcttaaaat
    19861 aaaaattttt aatataattt gttctatagt acatctattt tgattagttt acataatttt
    19921 attattttgt acaatattaa ttatttacta atatcccatt taatgaaaaa gttctaggtt
    19981 cagtaatttt taccataact aatttaccaa ttaaatcatc atttccacct tcgaaattta
    20041 cgagtttatt ttgtctagtt ctaccagcaa acttattttc attattttta cttcttcctt
    20101 caactagaac ttcaactatt ctatctttat atccatcatt tatttctgca gaaatcctat
    20161 ttacagcttc taacacccta ttaaatctat catgctttat atcttctggt atttgatttt
    20221 ccattttagc agcaggagtt ccctgtcttt tagaataaat aaatgtaaaa gctgaatcat
    20281 atctaacttt ttcaaccaca tctaaagtgt ccaataaatc ttcttctgtt tctcctggga
    20341 aaccaatcat taagtctgta gaaaaagcaa tacctggaac ttccttttta gctttttcta
    20401 ttatttctaa ataatattcc tttgtataat gtctattcat ttttttcaaa aggctactgc
    20461 ttccacattg tattggtaga tgtaaaaact cacacacctt atcacaatct ctaattgcgt
    20521 atattacttc atcagatata tcttttggat gagatgtcat aaatcgaacc ctttcaattc
    20581 cttctatatc attcactttt cttaaaagtt ctgagaatga aactggattt tcaagtgtct
    20641 taccataaga atctacattt tgtccaagta aagtgatttc tttcgttcca tttgcaacaa
    20701 gctcttttat ctcgttaatt atatcttcag gagttctact tctttctctt cctcttgtgt
    20761 atgggactat acaatatgtg cagaaattat tgcatccata cattatattt acaaatgctt
    20821 ttaattcaaa ttttctatta cttctaagac cttctattac ttctccatcc acatcccata
    20881 catccactaa cattttgtca gaatttattg attctgtaag taattgcgga aatttatata
    20941 gattatgtgt tccaaatata aggtctacat gtttatattt ttttcttaat tcctctacaa
    21001 catgtggttg ttgcatcata catccacata cagctatttt catatctgga ttttttcctt
    21061 ttagatgttt taattgtcct aagttaccat acacttttag ctctgcattt tctctaactg
    21121 cacaggtgtt atatattatt aaatcacttt cttcaaccat cattgacatt tggtatccca
    21181 tctcttctaa cattgaacat aatttttctg aatcatgttc attcatttga cacccgaagg
    21241 tttgagtaaa cacgagtttt ctctttccag ttctgttgaa gtattcttca ttttgtctgt
    21301 gtatttgttc tatatattta tcttgttctt gtattttctc tataggtatt gttacttgtt
    21361 ttcttttgct cacgtctatt cctcctagtt aatttcttat attacacata accttaatta
    21421 tatcaaataa gaaaaatctt ttaaagatat tattattttt ctagttgtgt tttaatagta
    21481 aatctaatga tttatatata ctatatttct aaaatataaa atcatataat tattataata
    21541 ataaaaagat gttttaaatt aaaacttaaa acacctttcc ttaaataaaa tatatgattt
    21601 actattaaat acaattacat ctattcattt tcaattaaat aacaatcatt taatattaaa
    21661 tattgatctt cctcaaccgt tatatatctt cttcctgaaa aagtatcatt agtaactatt
    21721 ccctctgcat tacctaagct atcatttcta acttcataat atgcatcaga agaatttgac
    21781 attataatat attctccagc aggaatttct aatccaattt tatacatacc atttccaaac
    21841 ttaccattct tggccttctg ggctttagca tactttgcta atgtcattgt tgaatcctca
    21901 attttaatat attgaccatt ctttaaggtg atatatctag tcccactaaa aatatcattt
    21961 gaaagtatac tatcagcatt acctgtacta tcacttgtaa cttcataata agaagcataa
    22021 cttcctgagt ttgaagttat taagtattca cctgctgaga tatcttttcc aaccttatac
    22081 attccatctt ttatagtctt tgtagtaggt ttaacatttt ttacaacccc agtattaata
    22141 tctccaattc catttgttat atttaaacac tgttgtacta tatttgaatc tatatatcct
    22201 atagaagtta tcttcttagc attttttaaa atgcttttat tgctaccatc gttaactaaa
    22261 accattggac catgttttga taaagaagat cctacaagtg catttgttaa ttcataagct
    22321 ccagctatat aaaactctct agcatcctta taaaacttat ttattattgc tttattagtt
    22381 tcaaaacgag tactcccccc tagtctagta gatttagtat tgtttactaa agtggtactc
    22441 attgaagctg ttcctcctat tacataagac tttaaaccac tagttgaaaa aggtatactt
    22501 tgtccattag taagaattat tggtgcttta tctcttgctg ctacagatgc aatacttata
    22561 gcatctgctt ctcctttata tgcattggtt agcataacag tattgacttt ttttattgaa
    22621 tttatttctt ttgcaacatt ataacttgtt tttattctgt catttccctt aattcttaca
    22681 actttcattt tcttagattt taatgaattt tcgacagatg tactaataga atatgttcct
    22741 ccaattatat aaacttttga tacatttttt aatcttgcag atgtctcagt tggtatagta
    22801 tttttttcag ttaatagtat aggggcattt aaagctcctg ctagtccact tgcacttaat
    22861 ccatctgcaa tagagttact agtattaatt aatatagcag ttttatatgc agttttatat
    22921 gcagttttat atgcagtttt atatgcattt ttatctgcta ttttacctgc tatttcatac
    22981 ttattttttc cttgtatttt ttctacagat gatattgcat ctactaattg aatatttgca
    23041 aatacaaaag aaatacttaa agtcaatgtt aatattcttt tatagaattt cataataaaa
    23101 ttgtccccct cataataaat ttttaatata taaatacata ttct
//
LOCUS       DAELLV010000036        22104 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.036, whole genome shotgun sequence.
ACCESSION   DAELLV010000036 DAELLV010000000
VERSION     DAELLV010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22104
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.036"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            392..1225
                     /locus_tag="KPD58_001188"
     CDS             392..1225
                     /locus_tag="KPD58_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HBG0264970.1"
                     /translation="MRNKKKKINRKKLYLLLSAIVVCLAFIVVGVRVSSLNMKENEIS
                     RNAKLSSSTITDIVSNTSIEVSKGPSKSSGKIAYITIDDGPSKFTDEMIKTLNKYNVK
                     ATFFMIDGNMKEYPQQVKNIIKNGNTAGFHSVSHDIHKLYVTSTSAKEEFDTNDQTFY
                     KITGKHSKVIRIPYGSKPYTPQASYQALVDAGYKIWDWDLDTEDWRSNSSQIVQNVKN
                     HIKNRKGEDKDQLVVLMHEKKQSAEALDSVLKFLSDEGYEFAPVDQNQIPKNYWLRNL
                     E"
     gene            1318..2250
                     /locus_tag="KPD58_001189"
     CDS             1318..2250
                     /locus_tag="KPD58_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG0264971.1"
                     /translation="MVGILSLMMVGCNKSLAKVNDVEITKEQYKKTKAVLSATNNYIN
                     GQSLDELEKTLDKKGRNKLENVIISFMVDNELLYQEAKDKGLTPSKSEVDSKYQELED
                     KMNLNTSYKEKMDKAGVDKDYLKQEISRDLAIDKNKKAFEDRINISDNDMEAYYTSHK
                     KDFNVEEVSASQILISTLDKNKKEVSKDKKEALKKKADNILTKIKNGESFESLAKKYS
                     DDKATGKNGGQLGYFTKDDKNAEFTKEVFKLKKNEVSNVFETSYGYHIVKVTDKRERQ
                     KSFNECQSLIRESILNEKYIEHIKKLNEDAKIDR"
     gene            2302..2784
                     /locus_tag="KPD58_001190"
     CDS             2302..2784
                     /locus_tag="KPD58_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG0264972.1"
                     /translation="MFKSLKRTKVEKYIIDNKDSFYRIAYSYTKNEEDALDVVQEAMY
                     KALYSVENIKEVNYIKTWFYKILVRTSIDFIRKNRKYNNMTDIDLIDETGEYDKYTDL
                     DLRRALEELPIEYKSIIILRFFEDLKIEEVAIILDENVNTVKTRLYTALKKLKLKIEE
                     "
     gene            2796..3665
                     /locus_tag="KPD58_001191"
     CDS             2796..3665
                     /locus_tag="KPD58_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBG0264973.1"
                     /translation="MTSKERLNKLKDEYHKIPIPKKLDTIINHEKIENIYREKNKKVN
                     RLRFKVAIAFACIFTVLVNISPVFADNFSKIPVIGAIVEVITIKNYSLKSENYEAEID
                     IPKIRGLKDKNLEQRLNSSFMEDGKRLYHQFQERMEKIQSSKNKGYKSLSLSYSVKNN
                     SKKFLSIEMTKNEIEASSYVSKVHYTIDKKRQIVLTLPMLFKDDKYIKVISDNIKEQM
                     REQMKKDSTKSYFIDQKKDLPVEDFKTINKYQDFYFNKNEDLVICFDEYEVAPGYMGA
                     VEFVIPYKVIKDL"
     gene            3708..4463
                     /locus_tag="KPD58_001192"
     CDS             3708..4463
                     /locus_tag="KPD58_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBG0264974.1"
                     /translation="MTTYKKLKKSIFKIAIFSICLLVSLVDTNPIFAGSSFNETSNSD
                     TIKVLNTKSINFKQDNYEVNVSIPKIKGLKDKTLENKLNNEFLQDGKKIYSEFQNKMK
                     KEDNKGHRYLNVIHNVKNNSKDFLSIEMTKEEIEASSSITKKHYTVDKNRGILLNLPI
                     FFKDSRYIRAISDNIKEQMRNQIKKDDTKSYFIDQDKDSPVKDFESINKYQDFYFNKN
                     GNLVISFDEYEVAPGYMGAVEFVIPNKVIKRLR"
     gene            4505..5326
                     /locus_tag="KPD58_001193"
     CDS             4505..5326
                     /locus_tag="KPD58_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HBG0264975.1"
                     /translation="MNIYEQAIRLIENNEDFAFATITSHSGSTPRETGAMMIVKNDST
                     IFGSVGGGSVEAECIKHAINVIKNRESMLYKFTLNKSDVAKLGMICGGTGEIQIDFID
                     SKLKSNIEKFNKRLKENTSKAYIFGAGHISRDVAVILSLLEFRTVVIDDREEFANHER
                     FPESEVVVLDSFENIPDFPTDKNSYIIILTRGHLYDSSALEWALKREAGYIGMIGSRT
                     KIGLTYEKLMKKGFKKEELSKVHAPIGIKLNAQTPAEIAVCIAAELINCRANKEK"
     gene            5331..6092
                     /locus_tag="KPD58_001194"
     CDS             5331..6092
                     /locus_tag="KPD58_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HBG0264976.1"
                     /translation="MNTGSKDIDTLKVNFRGIEIKAVLVYKNRKNISIKIDPFGKITV
                     MSPPKISKKIIKDIIIEKGDWILKKSEKCKGREEVYKQRMFITGEKFLYLGEEYHLVI
                     KELLRDSVKKSNCRITINEYQIVVQTNDTSTDFIKKSLKSWYKMESERIVLERIDFLK
                     LNCEIMKQLVPASVKVKEQNKRWGSCTAQKNIYINSKISMARLDVIDYVIIHEFSHLV
                     HMNHSKKFYDLVKSIMPDYKDKENWLKINGYKIII"
     misc_binding    6253..6510
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6623..6862
                     /locus_tag="KPD58_001195"
     CDS             6623..6862
                     /locus_tag="KPD58_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBG0264977.1"
                     /translation="MENTVYAKLNQGIDSFLRVAMTLRRREVDIQSISMTVDNMNNSG
                     IKLIVNEEKTSLDSVLNHMKKLHDIREITVERMRH"
     gene            7166..8161
                     /gene="ilvC"
                     /locus_tag="KPD58_001196"
     CDS             7166..8161
                     /gene="ilvC"
                     /locus_tag="KPD58_001196"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HBG0264978.1"
                     /translation="MKMARMYYEKDVDLEVLKNKKVAVLGYGSQGHAHAQNLRDNGVH
                     VVIGLYDGSKSAQKAKEDGFEVKSVAEATKESDLTMMLMPDEKQKKVYEESVKDNLKE
                     GQTLAFAHGFNIHYNQVQPPEFVDVVMVAPKGPGHLVRNVFTKGSGVPALFAVYQDYT
                     KKATETVLAYAKGIGATRAGVLETTFKEETETDLFGEQSVLCGGISELIKLGYKTLVD
                     AGYQKEVAYFECLHEMKLIVDLIYEGGFERMRYSISDTAEYGDYVSGKRVITDVAKQG
                     MQNVLEDIQNGKFAKAWIKENEEGRENFLKTREEEYNTEIAEVGRNLRSMMSFLK"
     gene            8214..9902
                     /gene="ilvB"
                     /locus_tag="KPD58_001197"
     CDS             8214..9902
                     /gene="ilvB"
                     /locus_tag="KPD58_001197"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HBG0264979.1"
                     /translation="MNGAKVILECLKKEGIDTIFGYPGGAVIPLYDALYDYSDDFKHI
                     RTSHEQGLVHAADGYARSTNTVGVCFTTSGPGATNAITGIATAFMDSSPMVVISGQVP
                     TSLLGKDSFQEIDITGATLSMTKHNYLVRNTKELVPTIKEAFRVANSGRKGPVLVDVP
                     KDLFLAEMDFSGEDYDLCQIDDYMDYKSDFDLDDETNIKLLNEAIDIIKESKKPIIYA
                     GGGVKSSDSEEILEKFATKIDTPVLNTLMGLGNIDRKNELSLGMVGMHGSRESNLALS
                     NSDLVIAIGARFSDRVISKSSEFAKNAKIIHIDIDPSEISKNIESNVSLVGDVKLVLS
                     LLIERVESKNNSNWKEEIKRFRKSEGVQTYEFHPQNILKKINEKYETLKKPTVVVTDV
                     GQHQMWAAKYWNFKGNKSFITSAGLGTMGFGLGAAIGTKVGNVDKNVVLVTGDGSFRM
                     NCNELATVANYNVPMLILLLNNRTLGMVRQWQKLFSNQRYSQTDINENVDYVKLVNAY
                     NIDGYKVSSMEELGKALDMIDFNKPVFLQCDIDKDYDVYPIVAPNDALENLICN"
     gene            10113..10790
                     /locus_tag="KPD58_001198"
     CDS             10113..10790
                     /locus_tag="KPD58_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBG0264980.1"
                     /translation="MFKHDKALLKEVKVERPNPQYAVLMQEQLGGANGELKAAMQYLS
                     QSFRIKDPQIKDLFLDIAAEELSHMEMVAQTINLLNGHDVDYNAVNTGEIETHVLTGL
                     SPVLINSSGAPWTANYVTVTGDLVADLLSNIASEQRAKVVYEYLYRQIDDKYVKETID
                     FLLNREEAHNALFRDALNKVKDTGSNRDFGVTEDSKLYFDLSTPGPNHDTKIDINPPS
                     FEKPLKK"
     gene            10964..13000
                     /locus_tag="KPD58_001199"
     CDS             10964..13000
                     /locus_tag="KPD58_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HBG0264981.1"
                     /translation="MDSNRKPNNLINEKSPYLLQHAYNPINWYSWNDEAFKKAKEEDK
                     PIFLSVGYSTCHWCHVMEKESFEDEEVAEIMNRNFVAIKVDKEERPDVDSVYMTVCQA
                     MTGSGGWPMTIIMTPDKKPFFAGTYFPKYSRYNRPGVIDLLENVSEKWNTSRDILIKS
                     GDEIIEALKDDFGVKNTEGDLSKEMLSSSVRVFKAIYDEKYGGFGNAPKFPSPQNLMF
                     LMKYYSIEKDKDVLKMVEKTLDGMYRGGLFDHIGFGFSRYSTDKKWLAPHFEKMLYDN
                     AMLTIAFLDAYKITKKELYKEIAIKTIDYVVREMKDKEGGFYSAQDADSEGEEGKFYT
                     FNPLEIIEVLGEEDGTFFNNYFDITSSGNFEGKSIPNLIKNKEYERHNEKIADLSKKV
                     FEYRKERTSLHKDDKILTSWNALMIVALTKAYSTLKNDIYLEYSNKCLNFINNNLVNE
                     SGRLLARYRDGSSDYLAYLDDYAFLIWAYIELYESTFNMKYLEKALNLNESCINLFWD
                     YEKSGFYIYGKDSENLIARPKDLYDGAIPSGNSVQLYNLIRLAKITGDNRLEEMSYKQ
                     LKLYVDNVKSSPTGYSFYMLSLMFELYSTKEIICIFKEDSDLIAFKELISENFIPNAT
                     FLAKKYNEENTIIGFLNNYRLKDDKISYYVCQSNSCSQPINDLQKLKDMILGVE"
     gene            complement(13191..13757)
                     /locus_tag="KPD58_001200"
     CDS             complement(13191..13757)
                     /locus_tag="KPD58_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0264982.1"
                     /translation="MDYSNELKELFLMNQTYATLFTLTNKIQIEGDKYFGILTSRQYM
                     TILSILHLPEEETTLNNIARKMGTSKQNINRLVANLEKNGYVDVIPSPHDKRAINVKV
                     TDLGKKVMVTCSRTGINFMADVFHEFTKDELETLWSLLKKMYRFNGEEQDGFEEDANF
                     MEYEEIDKIKSEALEEFAKRRNRVNKKD"
     gene            14045..14980
                     /locus_tag="KPD58_001201"
     CDS             14045..14980
                     /locus_tag="KPD58_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0264983.1"
                     /translation="MKCTNFTFKGEEGLDIYTYKWEDENIKKPKAVIQIAHGMAETAQ
                     RYETFAKVLTKNGYIVYINDHRGHGKTAKIIENVGHLAEKEGFRCLVEDMYTLTNIIK
                     KENEDLPIYLFGHSMGSFASQRYIMDYSNNLSGLILCGSNGKQGIILNLAHLIINREI
                     KKYGRRSKSNKINNLIFGGEIIRRNEKTKFDWLSRDKEQVEKYINDPFCGVVCSCGFF
                     YDLVQGLKEIEDKENLKKVPLDIPIYIISGDKDPIGKNGKGVLRLRDRYIKLGVKDVT
                     CKLYKDGRHELLNEINREEVFEDIICWLNNKIEIL"
     gene            15451..16665
                     /locus_tag="KPD58_001202"
     CDS             15451..16665
                     /locus_tag="KPD58_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0264984.1"
                     /translation="MQIKIGKVIQCLRKERNLTQEQLAKFIGVSTPAVSKWESGNSYP
                     DIELLPLLADFFNVSIDKLLNYKIDLSEEEVMKIYKELESGFARIEIDLSTEEPKEEF
                     RQDLESVKKLSNMYIEKYPKSYLLKLRICSLYQMYSYKFGKSELNDRVKETTNILEDI
                     VRNTDDIQIKETALIILSNAYCMLEDYEKAELYLNMIHKSIGDTSVNLAMIYLKQNRL
                     EEAEILLQNKLFSNVFNISMDCKGIINVYKNQYKELKKKLENRNFNKNAIEREMEYIK
                     NKLLGYANLSLEIKKMLSEDKGAFFSMYMDYMELSLIFLFFNMKEEAKKALYSLKEIL
                     EKYPIHESLDVSQMRFFDKVESKNLYTFNIYTNLLIVLNDDSYNELREEPIFKDVIEK
                     ILDMEKMLKNKE"
     gene            complement(17085..17606)
                     /locus_tag="KPD58_001203"
     CDS             complement(17085..17606)
                     /locus_tag="KPD58_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0264985.1"
                     /translation="MKFEWLRTSENKLYYKAMELYKISFPFHEQRKSSLQAEILKNKE
                     YQFNLIYDKNQFVGIILCWETDDFIYVEHFCIFPEMRNNRYGQRALELLNKKGKTIIL
                     EIDLPIDEISIYRKAFYERVKYKTNNYEHIHPPYHKKFNGHNLIVMSYPEKLSKMEYD
                     KFNHYLRNNVMSF"
     gene            17962..18759
                     /locus_tag="KPD58_001204"
     CDS             17962..18759
                     /locus_tag="KPD58_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HBG0264986.1"
                     /translation="MEKREKRIIISSLLSVSILMGLVSIYSILNKEDIILTVKGQEQK
                     VSSFKKTVEELLDEQGVKYNSEDKINPSLDTELKDDMKIKVVKVTKSKKEEIEKIPFD
                     TKHVNDSNLLKGKSKVYQEGQEGEKKLVYNLTYHDGKLVKKVLSKEVISKEPTTKIIK
                     YGTKEKVLIASRGANIRGGKHMKVVATAYAGDTITSTGTTPRWGVIAVDPRVIPYGTK
                     VYIPKLGMTFVAEDCGGAIKGNRIDIFMNSEGKASNWGRKSIDIYLH"
     gene            18869..19459
                     /locus_tag="KPD58_001205"
     CDS             18869..19459
                     /locus_tag="KPD58_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBG0264987.1"
                     /translation="MKIVCLGDSLTYGFGVSRSNSWTNIVNKETRLEIVNKGINGDTT
                     SGMLARFNEDVVKNSPDIVFIMGGTNDFIAGAGNEIINSNIMAMVHQSYSKNIIPIIG
                     IPLKPDIPNVREDWSCFTDFNIVSQNLESYSYWIKKFCMTFNTNFVDFYSEYNKNMEL
                     EGYKKLYFDGLHPTKEGHRIMADIFINSINKYIEEL"
     gene            19525..21222
                     /locus_tag="KPD58_001206"
     CDS             19525..21222
                     /locus_tag="KPD58_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBG0264988.1"
                     /translation="MDYTKTYEEWIKGSYFDEDTKLELENIKNNEKEIEDRFYKDLEF
                     GTAGLRGIIEAGTNRINKYTVRRATFGLANYILENTTKEETSRGVVIAHDNRHKSRQF
                     CIESANTLAACGIKAYIFDSLRTTPELSFAVRSLNAIAGIVITASHNPPEYNGYKVYW
                     EDGAQVMPEIANAITEKVNSIHDYSTIPTLTEANKNLVVLLDESQDTKFIEAVKSQII
                     RKDLVKNVGKSFKIVYTPLCGTGNVPIRRALKEVGFENIIVVPEEENPDPNFAGLDYP
                     NPEEKKALNRGILLAKEKGADLVIATDPDCDRVGVAVKTTTGEYALLTGNQIGGMLTH
                     YIIEGLKENNKLKENPTIIKTIVTSEFGADIAKANNVDVLNVLTGFKFIGEKIKLFEQ
                     NKNRSYVFGYEESYGYLVGTHARDKDGVVSSLLISEMAAFYYSKGMSLYDGLIELYKK
                     YGFFKEQTISLTLKGIEGVEKIKEIISYFRENQIDYINSIKVVDKKDYKNGIDNLPKS
                     DVLKYFLEDESWVAIRPSGTEPKLKFYIAVKGASDIEADKKIQGLKKYIDDMVEKLK"
     gene            21436..>22104
                     /locus_tag="KPD58_001207"
     CDS             21436..>22104
                     /locus_tag="KPD58_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HBG0264989.1"
                     /translation="MFGIDKKLIEYIIAKSNYSELSSKSAEVSFFLMLSIFPFLIFTI
                     SSIAYIPILHLNKYIALFRNMMPEGAFAVLSSIIVSAIDNRNLKFLAVSFVLTMWTFS
                     RAVKALIKGMNRAYKVKETRSFFKILSISFLFTIMLLVLIFLSMIFLVYGEKIGYFIF
                     NLVGLDEIFIKIWDILRYTVGIITIIVIFTLLYKYTPNKKLTIKESAPGAIFATFAWF
                     LVSFL"
BASE COUNT         9037 a         2179 c         3806 g         7082 t
ORIGIN      
        1 taaatgagat tagaaaattt tatttgataa caaaaattag atagtatgaa tatataatgt
       61 atgtattaaa aatgtggcaa atagttgttt tgctatttat tatttgttta atatttctaa
      121 tttataaaaa aaaaattata aattttaaaa attataaatg acattttaac tagaattaat
      181 tgtaaaaaac actatataat actcaatttt caacattcta tacaattata cagaaattat
      241 acagcataat aatcaccgaa ataacaaatt ttaaaaatta agcaaaactt tttgtcgaag
      301 ttcaacgtct atatatatgt agtgtaaatt tttgggataa aatactacaa aaatataaat
      361 gtaaaaatat agtaatattg gaggtcgaac gatgagaaat aaaaagaaaa aaataaatag
      421 aaagaagttg tatttgcttt taagtgctat tgtagtgtgt ctggctttta tagtagttgg
      481 tgttcgcgtc agctctttaa atatgaagga aaatgaaatt agtagaaatg caaaactgtc
      541 aagttctact attacagata tagtatctaa tacatcaata gaagttagta aagggccaag
      601 taaaagttct ggaaaaatag catacataac tatagatgat ggtccatcta aatttactga
      661 cgagatgata aaaactttaa ataaatacaa tgttaaagca acttttttta tgatagatgg
      721 gaatatgaag gaatatccac agcaagttaa aaatataatt aaaaatggga acacagcagg
      781 ttttcatagt gtatcacatg atattcacaa gctttatgtt actagtacat cagcaaaaga
      841 ggaatttgat acaaatgatc aaacttttta taagataact ggtaaacatt caaaagtaat
      901 aagaatacct tatggaagca aaccatatac accacaagcc tcttatcagg ctttagttga
      961 tgctggatat aaaatttggg actgggactt agatacagaa gactggagat ctaattcttc
     1021 tcaaattgta caaaatgtaa aaaatcatat taagaataga aaaggtgaag ataaagacca
     1081 acttgtagtt ttaatgcatg agaaaaaaca gagtgcagaa gctttagatt cagttttaaa
     1141 attcttatca gatgaaggtt atgaatttgc gccagtagat caaaaccaaa taccaaaaaa
     1201 ttattggtta cgtaatttag agtagttaaa atataagaat attgattgat atttttagaa
     1261 attgagaata aaaatgagtg aggtgtaggt atattgaata aaaaacttta tataggaatg
     1321 gtcggaatat tgtctttgat gatggttggt tgtaataaaa gtttagcaaa agtaaatgat
     1381 gtggaaatca caaaagaaca gtacaaaaaa acaaaagcag ttttatctgc tactaataat
     1441 tatataaatg ggcagtcttt agatgaatta gaaaaaactt tggataaaaa aggtagaaat
     1501 aaattagaaa atgttataat atcgtttatg gtggataatg aacttttata tcaagaggct
     1561 aaggataaag gactaactcc aagtaaaagt gaagtagact caaaatatca agaactagaa
     1621 gataagatga atttgaatac aagctataag gaaaaaatgg acaaagcagg tgttgataaa
     1681 gattatttaa aacaagaaat atctagagat ttagcaatag ataaaaataa aaaggccttt
     1741 gaagatagaa taaacataag tgataatgat atggaagcct actatacaag tcataaaaaa
     1801 gattttaatg tagaagaagt aagtgcttct cagatactta tatcgacttt agataaaaat
     1861 aaaaaagaag taagcaaaga taaaaaagaa gctttgaaga agaaggcaga taatatattg
     1921 actaaaatca aaaatggaga aagttttgaa agtttggcta aaaaatattc ggatgataaa
     1981 gcaactggaa aaaatggagg acagctagga tattttacaa aggatgataa aaatgctgag
     2041 tttacaaagg aagtgttcaa attaaagaaa aatgaagttt caaatgtgtt tgaaactagt
     2101 tatggatatc atatagtcaa agttactgat aaaagagaaa gacaaaaaag ttttaatgag
     2161 tgtcagagtt taataagaga atctatttta aatgaaaagt atattgagca tataaaaaaa
     2221 ctgaatgaag atgctaaaat agacagatag aatttctaac agctcaatcc tagcctgtaa
     2281 tcggagggag attttacagg aatgtttaag agtttaaaga gaacgaaagt tgagaaatac
     2341 attatcgata ataaagactc tttttataga atagcatata gttatacgaa gaatgaagaa
     2401 gatgccttag atgtggttca agaagccatg tataaggcac tatactcagt ggaaaatatt
     2461 aaagaagtaa attatataaa gacatggttt tataaaattt tagttagaac gagtatagat
     2521 tttattagaa aaaaccgtaa atataataat atgacagata ttgaccttat agatgagact
     2581 ggagagtatg ataaatatac tgatttggat ttaagaagag ctttggaaga attacctatt
     2641 gaatacaaat ctattataat attaagattt tttgaagatt taaagattga agaagtagca
     2701 ataatattgg atgaaaatgt gaatactgtt aaaactaggt tatatactgc tttaaaaaaa
     2761 ctaaaattaa aaattgaaga gtagggataa aaagaatgac aagcaaagaa aggttaaata
     2821 agttaaaaga tgagtatcat aaaattccaa tacccaagaa attagatact ataataaatc
     2881 atgaaaaaat agaaaacata tatcgagaga aaaataaaaa agtaaataga ttaagattta
     2941 aagttgcaat agcctttgca tgtatattta cagtattagt aaatataagt cctgtatttg
     3001 cagacaattt ttctaagata cctgtaattg gagcaatagt agaagtaata actataaaaa
     3061 attatagttt aaaaagtgaa aattatgaag ctgagattga tattcctaaa atacgaggtt
     3121 taaaagataa aaacttagaa caaagattaa acagtagttt tatggaagat ggaaaaaggc
     3181 tttatcatca attccaagag agaatggaga agatacaatc atcaaagaat aaaggatata
     3241 aatctttaag tttgagttat agtgtaaaaa ataattctaa aaaatttcta tcaatagaga
     3301 tgacaaaaaa tgagatagag gcttcatcat atgtgagtaa ggtacattat actattgata
     3361 aaaaaagaca aatagttttg actttgccaa tgctttttaa agatgataag tatataaaag
     3421 ttataagtga taatataaaa gaacaaatga gagaacaaat gaaaaaggat agtacaaaat
     3481 cctattttat agatcaaaaa aaagatttgc ctgttgaaga ttttaaaaca attaataagt
     3541 atcaggattt ttattttaat aaaaatgaag accttgtaat ttgttttgat gaatatgaag
     3601 tagcacctgg ttatatgggt gcagttgagt ttgtaatacc atataaagta ataaaagatt
     3661 tgtaaattga atagtagttt atatataaat attttaagaa ggacaatatg actacatata
     3721 aaaagcttaa aaaatctata tttaaaattg ctatattttc aatatgctta ttagtttcat
     3781 tagtagatac aaaccctata tttgcaggta gttcctttaa tgaaacttca aatagtgata
     3841 cgataaaggt gttaaatact aagagtatca actttaaaca agacaattat gaagtgaatg
     3901 tgagtattcc taaaataaaa ggtttaaaag ataaaacttt agagaataaa ttaaacaatg
     3961 agtttttgca agatgggaaa aaaatttaca gtgaatttca aaataaaatg aaaaaggaag
     4021 ataataaggg tcatagatac ctaaatgtaa tccacaatgt caaaaataat tctaaggact
     4081 ttttatcaat agaaatgaca aaagaagaga tagaagcttc ctcatctata actaagaagc
     4141 attacactgt tgataaaaat agaggaatac tattaaattt accaatattt tttaaagata
     4201 gtagatatat tagagctata agtgataata taaaagagca gatgagaaat caaattaaga
     4261 aagatgatac aaaatcttat tttatagacc aagataaaga ctcgccagtg aaagattttg
     4321 aatctataaa taagtatcaa gatttttatt ttaataaaaa tggaaatctt gtgattagtt
     4381 ttgatgaata tgaagtagca cctggttata tgggagcagt agaatttgtg ataccaaata
     4441 aagtaataaa aagactaaga tagatatata gaagattttt agatagactg gtaaggagaa
     4501 ttatatgaat atatatgaac aagcaataag attaatagaa aataatgagg atttcgcatt
     4561 tgctacaata acgtctcatt caggttcaac accacgtgaa actggagcaa tgatgattgt
     4621 gaaaaatgat tctactatat ttggctcagt aggaggaggt agtgtagaag cagaatgtat
     4681 aaaacatgca attaatgtta taaaaaacag agaatcaatg ttgtataaat ttactctaaa
     4741 caaatctgat gtggcaaaac taggaatgat atgtggaggt actggagaaa tacaaataga
     4801 ttttatagat agtaaattaa aaagtaatat agaaaaattt aataagagac taaaggaaaa
     4861 tacaagtaaa gcatatatat ttggtgcagg tcatatttca agagatgttg ctgtgatact
     4921 atctctttta gaatttagaa cagtagtaat agatgataga gaagaatttg ctaatcatga
     4981 aaggtttcca gaatcagaag tagtagtttt agattctttt gaaaacatac cagattttcc
     5041 aactgacaaa aatagttata taattatttt aacaagaggt catctatatg attcaagtgc
     5101 tttagaatgg gctcttaaaa gagaggctgg ttatataggt atgattggaa gtagaacaaa
     5161 aattggattg acttacgaaa aactcatgaa aaaaggtttt aagaaggaag aattatcaaa
     5221 agtacatgca ccaataggca taaaattgaa tgctcaaaca ccagctgaaa tagctgtatg
     5281 tatagctgct gaacttataa attgtagagc taataaggaa aaataaacta atgaacacag
     5341 ggagtaaaga tattgatact ttaaaggtta attttagagg gattgaaata aaagcagttt
     5401 tagtatataa aaatagaaaa aatatttcta taaaaataga tccatttggc aaaataactg
     5461 taatgagtcc tcctaaaata tccaaaaaaa taataaaaga tataattatt gaaaaaggag
     5521 actggatact taaaaagtca gaaaaatgta aaggaagaga ggaagtatat aaacaaagaa
     5581 tgtttataac tggcgaaaaa tttctttatt taggtgaaga gtaccattta gttataaaag
     5641 aactgttgag agattctgtc aaaaaaagta actgtagaat aactattaac gaatatcaaa
     5701 tagttgttca gacaaatgat acaagtacag attttattaa aaaatcttta aaatcttggt
     5761 ataagatgga aagtgaaaga atagtattag agagaataga ttttcttaaa ttaaattgtg
     5821 aaataatgaa gcaattagtt ccagcgagtg ttaaggtaaa agaacaaaat aagagatggg
     5881 gaagttgtac agctcaaaaa aatatttata taaactcaaa aatatctatg gcaagattag
     5941 atgtaattga ttatgtaata atacatgagt ttagccattt agtacatatg aatcactcta
     6001 aaaaattcta tgatttagta aaatccatta tgcccgatta taaagataaa gaaaattggc
     6061 taaaaataaa tggatataaa ataataatct gaaaacgatt attccaaaat ataggcgtaa
     6121 agtataaaag aagtattatt gtgaaaaaaa taaataatct gaaaatttta aaaaaagtca
     6181 ttgacattgt aaatgatagg gaatataatc tatattaaca acaaaacaga attaaaatat
     6241 aaaattaaaa aattaaaagc aatgacagag acaacaatac taatagtgtt atccacagag
     6301 aaccaatgtt gctgagaatt ggtgttaaca gtagtataat gatcactctg gagcttttgt
     6361 actgaatatt tttagtaagt acaaacgtat gtcagcgtta catgactaag gttaaggtaa
     6421 ttaattaccc gacctgaaaa aagaggggta gatgtatttt ttatatctac aaataaggtg
     6481 gtaccgcgga atataacttt cgtccttata agtaatattt atattgctta taaagatgaa
     6541 agttttttta ttttaagcga tataaataaa tttaatccat ataaaattaa taaaaaaata
     6601 ataaaaataa ggagggattt ttatggaaaa tactgtatat gctaaattaa accaaggaat
     6661 agattctttt ttaagagtgg ctatgactct aagaagaaga gaggttgata tacaatcaat
     6721 aagtatgact gtagataata tgaataattc aggtataaaa ttaatagtaa atgaagaaaa
     6781 gacatcctta gattctgtct taaaccatat gaaaaaacta catgatataa gagagataac
     6841 tgttgaaaga atgagacatt aattaaatat attttataaa gctaaagata aatatgctta
     6901 ttttaaaata aaaaactaaa gataaatatt aaataagaaa aatcttagat taaaatataa
     6961 gtgtcattga atttattagt ttatcacagt taataaattt attagtttgt tgtagctaat
     7021 aaaaactgaa aattctaaaa ttataattgt aagattatat aaatttagaa tttaaatctg
     7081 ttgaataata ttaaaacaat gataatgagt atgaatttct aataaaaata aatatcataa
     7141 aaataagaaa aagtattggg aggaaatgaa aatggcaaga atgtattatg aaaaagatgt
     7201 ggatttagaa gttttaaaaa ataaaaaggt tgcagtttta ggttatggaa gtcaaggaca
     7261 tgcacatgca caaaatctta gagataacgg tgtacatgta gtgataggtc tttatgacgg
     7321 aagtaaatca gcacaaaaag caaaagaaga tggatttgaa gttaagagtg tagcagaagc
     7381 tacaaaagaa agtgatttga caatgatgtt aatgcctgat gaaaaacaaa agaaagttta
     7441 tgaagagagt gtaaaggata acttaaaaga aggccagaca ttagcttttg ctcatggatt
     7501 caatatacat tataatcaag tacaaccacc agaatttgtt gatgttgtaa tggtagctcc
     7561 taaaggacct ggtcacttag ttagaaatgt atttacaaag ggaagtggag ttcctgcact
     7621 atttgcagta tatcaagact acactaaaaa agctacagag acagttttag catatgcaaa
     7681 agggatagga gcaacaagag caggtgtttt agaaactact tttaaagaag aaacagaaac
     7741 agatttattt ggagaacaat cagtactttg tggtggaata agtgaactta taaaattggg
     7801 atataaaacg cttgtagatg caggttatca aaaagaagta gcatattttg aatgtttaca
     7861 tgaaatgaaa ctaatagtcg accttatata tgaaggtgga tttgaaagaa tgagatatag
     7921 tataagtgac acagcagaat atggagatta tgtatctggt aaaagggtaa taactgatgt
     7981 tgcaaaacaa ggtatgcaaa atgttttaga ggatatacaa aatggaaaat ttgcaaaagc
     8041 gtggattaaa gaaaatgaag aaggacgtga aaacttccta aaaactagag aagaagaata
     8101 taatacagaa attgccgaag ttggtagaaa tctaagaagt atgatgtcat tcttaaaata
     8161 aatatatatt aaatggatta gtttgaggga aagagtggtg tatagtagtg cgtatgaatg
     8221 gagcaaaggt aattttagaa tgtttaaaga aagagggaat tgacacaata tttggatatc
     8281 ctggtggagc cgtgatacct ctttatgatg ctctatacga ttattcagat gattttaaac
     8341 atataaggac atcccatgaa caaggtttag ttcatgctgc agatgggtac gcaagaagta
     8401 ctaatacagt aggagtttgt tttacaacat ctggtccagg agcaacaaat gccataactg
     8461 gaatagcaac agcttttatg gattcttcac ctatggttgt aatttcaggg caggtgccaa
     8521 ctagtttatt aggaaaagat tcttttcaag aaattgacat aactggagca acattatcta
     8581 tgacaaaaca taattattta gttagaaaca ctaaagaatt agttcctaca ataaaagaag
     8641 cttttagagt tgcaaattca ggtagaaaag gacctgtact tgtagatgtt ccaaaagatt
     8701 tatttttagc agaaatggat tttagtggtg aggactatga tttatgtcaa atagatgatt
     8761 atatggatta taagagtgat tttgatctag atgatgaaac taatattaag cttttaaatg
     8821 aagcaataga tatcataaaa gaatctaaaa agcctataat atatgcaggt ggaggtgtta
     8881 aatcttcaga cagtgaggaa attcttgaaa aatttgcaac taagatagat acacccgtac
     8941 taaatacact tatggggctt ggaaatatag atagaaagaa tgaattatct cttggaatgg
     9001 ttggaatgca tggaagtaga gaaagtaatt tagcactttc taattcagat ttagtaatag
     9061 cgataggtgc gagatttagt gatagagtga taagtaaaag ttctgaattt gctaaaaatg
     9121 caaaaataat acacatagac atagacccat ctgaaataag taaaaatata gaatctaatg
     9181 tatctttggt tggagatgta aagctagtcc ttagtttatt aattgaaagg gttgaaagta
     9241 agaataatag caattggaaa gaagagataa agagatttag aaagagtgag ggtgtacaaa
     9301 catatgaatt tcatccacaa aatatactta aaaaaataaa tgaaaaatat gaaacattaa
     9361 agaaacccac tgtggtagta actgatgtcg gtcaacatca aatgtgggct gctaagtact
     9421 ggaattttaa aggtaataaa agttttataa catcagcagg gttaggaact atgggatttg
     9481 gtttaggtgc agctattgga actaaagttg gtaatgtaga taaaaatgtg gttttagtca
     9541 ctggtgatgg aagttttaga atgaactgta atgaactagc tacagttgca aattataatg
     9601 tacctatgct tattttacta ctcaataata gaacattagg aatggtaagg caatggcaaa
     9661 agttattttc caatcaaaga tactctcaaa ctgacattaa tgaaaatgtg gattatgtta
     9721 aactcgtaaa tgcatataac attgatggat ataaggtatc cagtatggaa gaattaggaa
     9781 aggcgctaga tatgatagat tttaataaac cagtattttt gcaatgtgat atagataagg
     9841 attatgatgt ttatcctatt gtagcaccta atgatgcatt ggaaaatttg atatgtaact
     9901 aaaacttata ctgaatattg ataaaatttt ttattaaatt tttattagta ttcagttttt
     9961 gttgtttaga gatagattgg cattttttat ttttgcaaat aaatcaatga ctatataatt
    10021 aagaacttat taaattttaa ttatacaatt tagaatagta ttacaactta agtaaataat
    10081 aattttgaat aataatttac ggaggtattc atatgtttaa acacgataaa gcattattaa
    10141 aagaagtaaa agtagaaaga ccaaatcctc aatatgctgt tttaatgcaa gaacagttgg
    10201 gtggtgcaaa tggagagtta aaagcagcta tgcaatactt gtcccaaagt tttagaataa
    10261 aagaccctca aataaaagat ttatttcttg atatagcagc agaagagctt agccatatgg
    10321 aaatggtagc acaaactata aatttattaa atggacatga tgttgattat aatgcagtta
    10381 atacaggcga aatagaaaca catgtattaa ctggtctatc accagtctta ataaactcat
    10441 caggagctcc ttggactgct aattacgtta cagttacagg ggatttagta gctgacttac
    10501 tttcaaatat agcttctgaa caaagagcta aggtagttta tgagtacttg tatagacaaa
    10561 tagatgataa atatgtaaaa gaaacaatag atttcttact taatagagaa gaagcacata
    10621 atgctctatt tagagatgct ttaaataaag ttaaagatac aggttcaaat agagattttg
    10681 gagttacaga agattctaaa ttatatttcg atttgtctac tccagggcca aatcacgata
    10741 ctaagataga tattaatcct ccttcttttg aaaaaccttt aaaaaaataa tttgatattt
    10801 tttatgttgt tttgttttag tagttataaa aattatttac tagaagaatt aattatatga
    10861 aaaattaaat atgtatagtt aaaaatgggt atacacttat tataagtgta tatctatttg
    10921 tttattgcaa aacatataat aaaataacaa atgaggtggt attttggaca gtaatagaaa
    10981 acctaataac ctaataaatg aaaaatctcc atatctttta cagcatgctt ataatcctat
    11041 aaactggtat agttggaatg atgaggcttt taaaaaagca aaagaagagg ataaaccaat
    11101 atttttaagt gtaggatatt caacatgtca ttggtgccat gttatggaaa aagaatcttt
    11161 tgaagatgaa gaagttgctg aaataatgaa tagaaacttt gtggcaataa aagtagataa
    11221 agaggaaaga cctgatgtag atagcgtgta tatgacagtt tgccaagcta tgacaggtag
    11281 tggtggatgg ccaatgacca taataatgac accagataaa aaaccttttt ttgcgggaac
    11341 atatttccca aaatattcaa ggtataatag gcctggagtt atagatttat tagagaatgt
    11401 atctgaaaag tggaatacaa gcagggatat attaataaaa tctggagatg aaataataga
    11461 agctcttaag gatgattttg gtgtaaaaaa tactgagggt gatttatcaa aagagatgtt
    11521 gagttcatca gttagagtat ttaaggctat ttatgatgaa aagtatggtg gttttggtaa
    11581 tgctcctaaa tttccaagtc ctcagaatct tatgttttta atgaaatatt atagtataga
    11641 aaaagacaaa gatgtgttaa aaatggtgga aaaaacttta gatggaatgt atagaggagg
    11701 cttgtttgat catattggat ttgggttttc aagatactct actgataaaa agtggttggc
    11761 tcctcatttt gaaaaaatgc tatatgataa tgctatgctt actatagctt ttttagatgc
    11821 gtataaaata acaaagaaag agttgtataa agaaattgct ataaaaacta tagattatgt
    11881 agttagagag atgaaggata aagaaggtgg gttttattca gcacaggatg ctgatagtga
    11941 aggtgaagaa ggtaaatttt atacatttaa tcctcttgag ataattgaag ttttgggtga
    12001 agaagatgga actttcttta ataattattt tgatataact agtagtggaa attttgaagg
    12061 caaaagtatt cctaatctta taaaaaataa agaatatgag agacataatg aaaagatagc
    12121 tgaccttagt aaaaaggtat ttgaatatag aaaagaaaga acatctttgc ataaagatga
    12181 caagatactt acatcatgga atgcattgat gatagttgct ttaacaaagg catatagtac
    12241 tttaaaaaat gacatatatt tagagtattc aaataagtgt cttaacttta taaataataa
    12301 tcttgtaaat gagtcaggta gattattagc tagatataga gatggaagtt ccgactattt
    12361 agcatattta gatgattatg cttttttaat ctgggcgtat atagagcttt atgagtcaac
    12421 atttaatatg aaatatttag aaaaggcttt aaatttgaat gaaagctgta tcaacctttt
    12481 ttgggattat gaaaaaagtg gattttatat atatggtaaa gatagtgaaa atttaatagc
    12541 aagaccaaaa gatttgtatg atggagctat tccatcagga aattctgttc aattatacaa
    12601 tcttataaga cttgcgaaaa ttactggaga taatagactt gaagaaatgt catataaaca
    12661 attaaaactg tatgtggata atgtaaagag ttctccaact ggctacagct tttatatgct
    12721 ttcattgatg tttgaacttt attctactaa ggaaattata tgtattttta aggaagattc
    12781 tgatttaatt gcatttaaag aattgataag tgaaaatttt ataccaaatg caactttttt
    12841 agcaaagaaa tacaatgaag aaaacactat tataggtttt ttaaataact atagattgaa
    12901 ggatgataag attagttatt acgtgtgtca aagtaatagt tgtagtcaac caattaatga
    12961 tttacaaaaa ttaaaagata tgattttagg agtagaataa tcataatagc gaaaaaggag
    13021 atgctttaaa ataaaagtct aatcatctcc tttttttgta caaaaaacag aaaaagagtt
    13081 cctaagaaca cctaatctat gtaatgattg ttttgttaaa aaataaaaat gacattatag
    13141 ttatataaaa tattgtacta taaaaaagtg gtattataga tgcgatagta ttaatctttt
    13201 ttatttactc tatttcttct ttttgcaaat tcttcaagtg cttcagattt aattttatct
    13261 atttcttcat attccataaa attagcatct tcttcaaagc catcttgttc ttcaccatta
    13321 aaacgataca ttttttttag taaactccat aaggtctcta attcatcttt ggtaaattcg
    13381 tgaaatacat ctgccataaa gtttattcca gtcctactac atgtcaccat aacctttttt
    13441 cctaagtctg taactttaac attaattgct ctcttatcat gaggacttgg aattacatca
    13501 acatatccat ttttttcaag attagcaact agtctattta tattttgttt acttgtaccc
    13561 atttttcttg caatattatt taatgttgtt tcttcctctg gtaaatgaag aattgataaa
    13621 atagtcatat actgtcttga agtaagtata ccaaaatatt tatctccttc tatttgtatt
    13681 ttgtttgtaa gggtaaaaag agttgcatat gtttgattca ttaaaaagag ctcttttagt
    13741 tcatttgaat aatccatgaa atatatctcc tttattttta gatgttgttt tgtcgaaaac
    13801 agaatatcat gaattaattc taatgtcaat atggtgtcaa aatatattaa agtattactt
    13861 ttttatgtaa ctattaatta attaaattat tgtataattt tatgtaaaaa cttctatgta
    13921 ccaatgataa cttctatgag gaattaataa ttcttaaatt ttttagaaag attttgactt
    13981 gaaaatactt gtaatataat atataaatta atatactgaa taaaatcaat ctggagggat
    14041 aattatgaag tgtacaaact ttactttcaa aggagaggaa ggtttagata tatatacata
    14101 taaatgggaa gatgaaaata taaaaaaacc aaaggcagtt atccaaattg cacatggaat
    14161 ggctgaaact gcacaaaggt atgagacttt tgccaaggta cttactaaaa atggttatat
    14221 agtatatatc aatgaccata gaggtcatgg gaaaactgca aagataatag aaaatgttgg
    14281 acatttagct gaaaaagaag gatttagatg tcttgtagag gacatgtata ctttaacaaa
    14341 tattataaag aaagaaaatg aagatctgcc aatttatcta tttggacaca gcatgggttc
    14401 atttgcaagt caaagatata taatggatta cagtaataat ttatctggtc ttatactatg
    14461 tggttcaaat ggaaagcaag gaatcattct aaatcttgct catctgataa tcaatcgtga
    14521 gattaaaaag tatggaagac gttctaaaag taataaaata aataatttga tatttggcgg
    14581 cgaaataata agaagaaatg aaaagactaa gtttgactgg ttaagtagag ataaagaaca
    14641 agttgaaaaa tatataaatg acccattttg tggagtagtg tgtagttgtg gcttttttta
    14701 tgatttagtt caagggttaa aagaaattga ggataaagaa aacttaaaaa aagtgccact
    14761 tgatatacct atttacataa tatctggaga taaagacccc ataggaaaaa atggtaaagg
    14821 agtcttaaga ctaagggata gatatataaa attaggagta aaagatgtaa cttgcaaact
    14881 ttacaaagat ggaaggcatg aattgttgaa tgaaataaat agagaagaag tttttgagga
    14941 tataatttgt tggttaaata ataaaataga aatattgtaa taaaaaaata taatgatata
    15001 tttgctttaa atttatttat atataatatt tgaaaagtgg attatgtata tgcaaagtag
    15061 aaaagagagc ccagaagtga ttttttgcta taatgccatt tattgaatag gtgggtgtta
    15121 ttataaataa attattaaca ctcagtatta ttatcattgt atatggaatt tattttattt
    15181 aaaattcctt gaaagtttaa aagtttaaat atagtaagtt gtaataaaga gaaaatctaa
    15241 atatttaaat gctatttaat gtgaatctat taattaggtt atacttaaac ttaattaata
    15301 gattttatat ttcaagccaa tgagataggg ttttagatta aaatataacc aatggttaaa
    15361 ttacagacat tttgttaact ttccctcgat ttactagtaa atattttttt tatatactta
    15421 aattacaaca gatgtactag ggaggtatat atgcaaatta aaataggtaa agtcatacag
    15481 tgtcttagaa aagaacgaaa tttaactcaa gaacaattag caaagtttat aggtgtgtca
    15541 acaccagcag tatctaagtg ggagagtgga aattcttacc cagatataga attattgcct
    15601 ttattagcag acttttttaa tgtgtctatt gataagcttt taaattacaa gattgattta
    15661 agtgaagaag aagtaatgaa aatttataaa gaattagaat caggctttgc tagaattgaa
    15721 attgatttat caacagaaga accaaaagaa gaatttagac aagatttaga atctgtaaaa
    15781 aagctttcca atatgtatat agagaagtat cctaaaagct atttattgaa gctaaggata
    15841 tgttcgctgt atcagatgta ttcatataaa tttggtaaga gtgaacttaa tgacagagta
    15901 aaagaaacaa cgaatattct tgaagatata gtgagaaata cagatgatat ccaaataaaa
    15961 gaaacagctc ttattatatt atcaaacgca tattgtatgt tggaggatta tgaaaaggca
    16021 gagttgtatt taaatatgat acataagtca ataggggata caagtgttaa tttagctatg
    16081 atatatttaa agcaaaatag acttgaagaa gcagaaatat tgcttcaaaa taaattgttt
    16141 agtaatgtat ttaatataag catggattgt aagggaataa ttaatgtata caaaaatcaa
    16201 tacaaggaat taaagaaaaa attggagaat agaaatttta ataaaaatgc aattgagaga
    16261 gaaatggagt atataaaaaa taagttgctg gggtatgcaa atctttcttt agaaataaaa
    16321 aaaatgctca gtgaagataa aggtgcattt tttagtatgt atatggatta tatggaatta
    16381 tctctaattt ttttattttt taacatgaaa gaagaagcaa aaaaagcttt atatagtcta
    16441 aaagaaatat tagaaaaata tcctatacat gaaagtttgg atgtaagtca aatgagattt
    16501 tttgataagg tagagtctaa aaatttatat acttttaata tatatacaaa cttgttaatt
    16561 gtacttaatg atgacagtta taatgagctt agagaagaac caatttttaa ggatgtaatt
    16621 gaaaagattt tagatatgga aaaaatgttg aaaaataaag aataagtaaa aatttaatat
    16681 aggacttaaa gattaatcaa attaaaagaa agtgtttcaa aataaaaatt tgaatcacct
    16741 tttttgtcgg tcttaaacag ttatttagaa aaatagaggt ttcaaaatag aaatctgaat
    16801 tatctgtatt attacacaaa aatagaaacc taaatttata aaaatagaat aaagtttgtt
    16861 atataaaatg taattttgtc tataaaatct cacctgtttt cttaacttta agcgtgaaaa
    16921 agaggatgta tcatgaattt tttagttcat gatgcatcct gctttgtatg aaatccaata
    16981 tatctttatt atttaaacaa tggaaaagag tcttatttat atttggagac atctctttta
    17041 tttaattatg aagttttata cagttcaata aataggaatt atatttagaa actcatgaca
    17101 ttatttctta gatagtgatt aaatttatca tattccattt tagataactt ttctgggtaa
    17161 gacattacaa tcaggttatg cccattaaat tttttatggt atggtggatg aatgtgttca
    17221 taattatttg tcttatattt tactctttca tagaacgcct tgcgataaat tgaaatctca
    17281 tcaattggaa ggtcaatttc taagataata gtttttcctt tcttatttaa aagctctaac
    17341 gctctttgcc catatctatt attgcgcatt tctggaaaaa tgcagaaatg ttctacatag
    17401 ataaagtcat ctgtttccca acataaaatt atgccgacaa actggttttt atcataaatt
    17461 aaattgaatt gatattcttt atttttcaaa atttctgctt gcaaagaaga ttttctttgt
    17521 tcatgaaatg ggaaactaat tttatataat tccatagcct tgtaatataa tttattttca
    17581 ctggttctta accattcaaa tttcacttta ctcctcctaa tcacgaaaat tccaatttat
    17641 ttatgagttc attttaccat agtcagtatt tgcatacaat gtgttttaag ttgagtgtca
    17701 tttctttctt ttatttatgc ttatatctca tagtctaaga gataattgct taagacataa
    17761 ctaaaaacta ttatattaag ctttgataat ctaaaaacag tatatttatg gacttttcta
    17821 ggtctaaaag taatcttaat aattttagaa gttacaaaaa agttacataa atttggaaag
    17881 ctataatata catatataat ataaggttaa aactgtaatg aataatatta atttaaaatt
    17941 tgaacatgga aggagattat tatggagaaa agagaaaaga ggataataat atcatcttta
    18001 ttaagtgtat caattttaat ggggttggta agtatatatt ctatattaaa taaagaagac
    18061 ataattttaa ctgttaaggg tcaagagcaa aaagtatctt cctttaagaa aacagttgag
    18121 gaacttttgg atgaacaggg tgtaaagtat aattctgaag ataagattaa tccaagttta
    18181 gatacagaac taaaagatga tatgaaaata aaagttgtta aggtaactaa aagtaaaaaa
    18241 gaagagattg aaaaaattcc atttgataca aagcatgtaa atgatagtaa tttgttaaaa
    18301 ggaaaatcta aagtttatca agaaggtcaa gaaggagaaa aaaaactagt ctataattta
    18361 acttaccatg atggaaagtt agtcaaaaaa gtcttatcaa aagaagtaat atctaaggag
    18421 ccaactacaa aaattataaa atatggaact aaagaaaaag tactaatagc atcaagagga
    18481 gcaaatataa gaggaggcaa acatatgaaa gtggttgcca ctgcatatgc aggagataca
    18541 ataacatcta ctggtacaac tccaagatgg ggtgttattg cagttgaccc acgtgtaata
    18601 ccatatggaa caaaagtata tatacctaaa ctgggtatga cttttgtagc agaagattgt
    18661 ggaggtgcaa ttaaaggtaa tagaatagac atttttatga acagtgaagg aaaagcttca
    18721 aattggggaa ggaaaagtat agatatatat ctacattaaa ttagtgaata gaataacttt
    18781 acttgaagga caaaaaagta taaaatatga attaactaaa ctgttagatt attcttaaaa
    18841 cataaaaata tatctaaggg gggataagat gaaaatagtc tgcttaggag acagtttaac
    18901 gtatggattt ggagtatcac gaagtaattc atggacaaac attgtaaata aagagactcg
    18961 attagaaata gtaaataagg gtataaatgg tgatacaaca agtggtatgt tagctaggtt
    19021 taatgaagat gttgtaaaaa attctcctga tatagttttt ataatgggtg gcaccaatga
    19081 ttttattgct ggtgcgggaa atgaaattat taattctaat ataatggcta tggtgcatca
    19141 gtcatattca aaaaatatta tacctataat aggtatacca ttaaaaccag atattccaaa
    19201 tgttagggaa gattggagtt gttttactga ttttaatata gtatctcaaa acttagaatc
    19261 atatagttat tggataaaaa aattttgtat gacatttaat actaatttcg tagattttta
    19321 ttcagaatat aataaaaata tggaactaga aggatataaa aaattatatt ttgatgggtt
    19381 acatccaacc aaagagggtc atagaataat ggcagacatt tttattaatt caattaataa
    19441 gtatatagaa gagttataga ttacattaaa atgcttgttt atgcgtcagt tgcaataaat
    19501 acatacagta gagaggggtt tattatggat tatactaaaa cttatgaaga gtggataaag
    19561 ggttcatatt ttgatgaaga tacaaagcta gagttggaga atataaaaaa taatgaaaaa
    19621 gaaatagagg atagattcta taaggattta gaatttggga ctgcgggtct tagaggaata
    19681 attgaagcag gaacgaatag aataaataag tacacagtta gaagagctac ttttggattg
    19741 gcaaactata tattagaaaa cacaacaaaa gaagaaacaa gtagaggagt agtaatagct
    19801 catgataata gacacaagtc tagacaattc tgtatagaat ctgcaaatac tttggcagca
    19861 tgtggtataa aagcatatat atttgatagt ttgagaacta ctccagaatt atcttttgct
    19921 gttagaagct taaatgcaat cgctggtata gtcataacag caagccataa tcctccagaa
    19981 tataatggat ataaggtata ctgggaagat ggagcacaag ttatgccaga gattgcaaat
    20041 gcaataacag agaaagtaaa tagtatacat gattatagta caataccaac cttaacggaa
    20101 gctaataaaa atttggtagt tttgttggac gaatcacaag atactaaatt tatagaagca
    20161 gtaaaaagcc aaattataag aaaagattta gttaaaaatg taggtaaaag ctttaagata
    20221 gtttatacac ctctttgtgg aacagggaat gttccaataa gaagagcatt gaaagaagtt
    20281 ggttttgaaa atataattgt agttccagaa gaagaaaatc cagacccaaa ttttgcaggg
    20341 cttgattatc caaatccaga agagaaaaaa gctttaaata gaggtatatt actcgcaaaa
    20401 gaaaaaggag cagaccttgt aatagcaact gacccagatt gtgatagagt aggtgtagca
    20461 gtaaaaacta ctacaggtga gtatgcttta cttacaggaa atcaaattgg tggtatgctg
    20521 acacattata taatagaagg attaaaagaa aataataaat taaaagaaaa tcctacaata
    20581 ataaaaacca tagttacatc agaatttgga gcagatatag ctaaagcaaa taatgttgat
    20641 gtattaaatg ttcttacagg ttttaaattt attggtgaaa aaataaaatt atttgaacaa
    20701 aataaaaata gaagttatgt atttggatat gaagaaagtt atggttactt agttgggact
    20761 catgctagag ataaagatgg tgtagtttct tcattgttaa tatctgaaat ggcagcattt
    20821 tactattcaa aaggtatgag tttatatgat ggtttaatag aactttataa aaagtatgga
    20881 tttttcaaag aacagactat atctttgact ttaaaaggta tagaaggtgt agaaaagata
    20941 aaagaaataa tatcttattt tagagaaaat caaattgatt atataaatag tattaaagta
    21001 gttgataaaa aagattataa aaatggtata gataatcttc ctaaatctga tgttttaaaa
    21061 tactttttgg aagatgaatc ttgggtagct ataagaccat ctggaacaga accaaaactt
    21121 aaattttaca tagctgtaaa aggagctagt gatattgaag cagataaaaa gattcaaggt
    21181 ttgaaaaagt atatagatga catggtcgaa aaattaaaat aaaatattat tattttataa
    21241 gataattttg taataatatt tatttagctg aaaattataa tttactttca ttgagtagaa
    21301 tatggaaatg ttagtagaat tgttattttc agcttttatt tgtgttattt ttacttaaat
    21361 aattatatta tagaatgaat ctgagtataa ttaatattgt ataatgaaat taatttgata
    21421 gaaaagggga tatttatgtt tggtatagat aagaaattga ttgaatatat aattgcaaaa
    21481 tccaattaca gtgaattaag ttcaaagtca gcagaagtat ctttcttctt aatgttgtcc
    21541 atatttcctt ttttaatatt tacaataagt tctatagcct atataccgat tcttcattta
    21601 aataagtata tagccttgtt tagaaatatg atgccagaag gtgcttttgc tgtgttatct
    21661 tcaatcatag tttctgcaat agacaataga aacctgaaat ttttagcagt cagttttgta
    21721 cttactatgt ggacattttc aagagcagta aaagctctta ttaaaggaat gaatagagcc
    21781 tataaagtta aagaaactag gtcttttttt aaaattttat ctatatcatt tttatttaca
    21841 ataatgcttt tggtccttat ttttttatct atgatatttt tagtttacgg ggaaaaaata
    21901 ggttatttta tttttaatct tgtaggattg gatgagatat ttataaaaat atgggatatt
    21961 ctaagataca ctgtaggtat aataacaata atagtgatat ttacactttt atataaatac
    22021 actccaaata aaaagttaac tattaaagaa tctgcgccag gtgctatttt tgctactttt
    22081 gcttggtttt tagtatcatt ttta
//
LOCUS       DAELLV010000037        21902 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.037, whole genome shotgun sequence.
ACCESSION   DAELLV010000037 DAELLV010000000
VERSION     DAELLV010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21902
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.037"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..719
                     /locus_tag="KPD58_001208"
     CDS             <1..719
                     /locus_tag="KPD58_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG0264990.1"
                     /translation="IILFLVLIILSFYTGFKVIRLINNNNEQEYKLQKLDSKINHKDN
                     IIIKLRSQKHDYLKHLQVVYSLLNTGLDEDAKEYIMNISNSFKSKNSRYGKVSYLDAI
                     ASLKYEECLEKDISFDVYIQDFMDNLEIEPNDMSSVLLNIIDNAIESLSTIKKEKKYI
                     RLHAYEDEFQYIIAIKNNGPKIHNVESIFWNGFSTKEGQDRGYGLHLVKNILDKYDYS
                     ILVNSDDFLTEFVILIPKYY"
     gene            738..1475
                     /locus_tag="KPD58_001209"
     CDS             738..1475
                     /locus_tag="KPD58_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0264991.1"
                     /translation="MIKVLICDDDKIVRDDIANIAKESGYVDYVKKVKDGIEAIEFIK
                     NEKIDLLIIDIDMPYKNGIDSAEEILSIDKDIHIVFVTGFADYSLESFKVHPIDFVVK
                     PFKKEKILDSINIAIDHINSHKIANNNFIEDTLFVYKIRKQIHMINFDDIVMFEKNSR
                     AINLHTKDEDIIKFYENFDDLKKRLPPNFFMTHRQYIVNLRFIHKVIPINKSSLEIRF
                     INFQENAILNKSLEKDFLYRFYRVKRF"
     gene            complement(1660..6717)
                     /locus_tag="KPD58_001210"
     CDS             complement(1660..6717)
                     /locus_tag="KPD58_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HBG0264992.1"
                     /translation="MLERTRVEKNPKIIENFKNNFTKSSSKQYYDLAKSLWDCIENSS
                     YYYREPEDKFLNMIKKYYNCHYYEIKNMDELLSGPLKNALELIFTSEQVEELCQLIKR
                     KMDYTYSGGYYRKGYRSNKLVDYFRGIIRFISNYVDLIYYDFSVIDYLTLPNKVLYYD
                     SIMSDKIALEIDKGNQKVISALEEVIFGENQTALLTRQMIIGIMKSHSDKCYEMLSKL
                     LLAAKQQEGLRQQIVESLDEGTISASIYMLKTILDNNLERFSSVIRALDTWTGLGFSD
                     QKPGIIRKCLELSYKSLTDEKFRIESINSNDNLEIYFALWATATYNIYDSYSLIENLL
                     SCQEKYKKLVALYFLSHTESTYFKHSIASKNIKGDDLEILAWVITNFYVEGSVGYYYS
                     WQNRETNFDLSNTPIDKDFDARKEQFESLKHTLEILNSKTKSFSGSVFPWTWIEISSH
                     EILKCMMTLTAYDMNAQFIDVLMENCHLMSSDLRASLIVNFVNEPKTDRQREFLLECL
                     SDKSVSNRETVLSLLRKLELTDVELDKITNLLSLKTSSIRQTVIKILLEQDDSNLENI
                     ASSLIKSSNSQKCLGGLEIVNLLKDNTARQELYNKLILLIEDIDKSKKNTSQVQILID
                     NLLGKAKNDYCKENGFGIYNPENKVQIPEQFDFKDDSNSSSKLNKIISNISLSTREDS
                     VSVATKKEAKKILDTDINKIIKVYEKFDELITENADYEYEVERWSGNVDKVLLGYSTY
                     NLTPIKYKVSNDTLDNYPLAELWRETSKKLDLDYKLILDILFYSISTCGYNETEKSWF
                     SSIYSNVLDSKKQNKIYTKLEKLKFNGLTRKLLRVLLNEFDHKEIFNYCLEIYYKLRN
                     LLTIEQCKKQRVEFTKNYYRQNEYCPALNAPILNYWLDKAHSFIYDDISFTKFFYTTF
                     SNYQLLEYKFPFYTSINVFARAHELGLMTEEQVNREMISGYNASNIIRTLTNPRWAGD
                     TLEKYPWLKDNLKTVTNRIIEIESRRGDMPTEVSNLANNIQYFEGINYFVNILVALGK
                     ETFQRGYSYGSNYSKKDVLSSLLKNCYPTKEDDSKKLKSLLKNTDITELRLIESAMYA
                     PQWVNIIEKYLNWKGLKSTIWYFHAHINEGFSAQKESEVALFSPISPRSFIDGAFDVD
                     WFLDSYKTIGEERFNILYKSAKYITSGGNSHRRAQLYADATLGKLDKDMLMKEIETNR
                     NQDKLRSFSLLPMKKGDLKEATFRYEFIQKFAKESKQFGAQRRESESKACTIALENLA
                     RSAGFGDVNRMMWALETEKLNEVAHLFKPKDIDGTSIWIDVDENGKASTKVQKGNKSL
                     KSIPKAIAKHPYVLELKSVQKDLKSQYSRAKQSLEQAMKNEIVFTVKEIANIYKNPVI
                     CPLIKNLVWVCDDNIGYVYNDKSKFFICNIEGETYKLTAKNKLKLAHPVHLFESGKWS
                     EFQHDLFSKQIVQPFKQVFREYYRINSDELSEKTISRRYAGHQIQPKKTVALLRNQLW
                     TVDYEEGLQKVFYKENIVAKMFALADWFSPADIEPPTIETVEFFDRKTYKSLELASIP
                     PILFSEVMRDIDLVVSVAHAGGVDPETSHSTVEMRIAIAKELLLLLKINNVNFEGSHA
                     LIAGNLGEYSVHMGSGIVHKKGMGALNILPIHSQSRGKIFLPFADEDPKTAEIMSKIL
                     LLAEDSKIKDPSILSNL"
     gene            complement(6742..7161)
                     /locus_tag="KPD58_001211"
     CDS             complement(6742..7161)
                     /locus_tag="KPD58_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264993.1"
                     /translation="MQIYFRVKAVGKRKPMLELTPFDVSDNINCLKEAISEIVSKNVL
                     EYNEKIPEKSIINFLTNEEIESQADIGKVGFGSIYNENKQSVEKAIEVALQAFEDGIY
                     KVLINEDVIEKLDSSINLKSGDIFTFIRLTLLAGRMF"
     gene            complement(7395..7646)
                     /locus_tag="KPD58_001212"
     CDS             complement(7395..7646)
                     /locus_tag="KPD58_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264994.1"
                     /translation="MNRYTKIINMMGSYYTKDFEKEKKNVIKVREVKEDTVRKFFLQG
                     DCEVLVVFEDTGKEILIDDFSPEEDIKKYLGAKFINKKR"
     gene            7735..8466
                     /locus_tag="KPD58_001213"
     CDS             7735..8466
                     /locus_tag="KPD58_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HBG0264995.1"
                     /translation="MTSIKNKDILNCIDYSIKNKLFDKLNDVYKSLPVGNCSGCGNCC
                     MESVGINLIEFLNIFCYLEDRVDLKKSCISKILDYYFEEYTRKNPCPFKDTNNRCLIY
                     EVRPLNCRMFGHWKKEDYNKNLDNVIEKNNNYRELMKKQYGFEINDEVVNYRIDYCDR
                     FIPNKDYLSKSKRLSFFDELMILDSNIYSSGNIDIDFRDRGIVEYFIESLLYRNLAYN
                     VKIRISKEYKIRKRTIDRIKRIVLV"
     gene            8525..9436
                     /locus_tag="KPD58_001214"
     CDS             8525..9436
                     /locus_tag="KPD58_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0264996.1"
                     /translation="MNLTKVKGNTFFIKGGTNTGIYVFEDNTALMIDPGLCGSRPRRV
                     NKVLDENNIQVRYIINTHGHDDHYGACNQFKEYYKDVCIMSSEYDKLYIENPELFSKY
                     IVGGKSNVFMDNILKNKMLDDIKIDKSIEAGKIELNNEMFDIIEFQGHTPGSIAVLTK
                     DNVIFVGDLLIGDEILSKYDFLFLYDIQEQINSLEKLKNIDFEYLVLGHGKQVISKED
                     SYKLIEKHLNALKKYLYQIRELLVEPKSLEILLKNIINNNNLSNNYKEYHFFKSSLVS
                     VISYLVDLEEIGYILENGELLYYTKTK"
     gene            9487..10299
                     /locus_tag="KPD58_001215"
     CDS             9487..10299
                     /locus_tag="KPD58_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HBG0264997.1"
                     /translation="MEYEKWNEILAPYEHAVEELKIKFKNIRKEYLAKGEYSPIEFVT
                     GRTKKISSIISKLKRINAKDIETEIDDIAGIRIMCQFVEDIYAIVDLIKVRNDMTIIG
                     EKDYITNYKDSGYRSYHVIIKYPINSIAGSKEIICEIQIRTLAMNFWATIEHSLKYKY
                     DHYIPETLAERLRRASDAAFLLDQEMSEIREDIMKAQAMYQMKSIAIRDTLNRIQELY
                     DLGDTHKAMQYQRKLDRTETDKNITEILELKQEIDILLEQYKDTKVDDKVVQ"
     gene            10408..11100
                     /locus_tag="KPD58_001216"
     CDS             10408..11100
                     /locus_tag="KPD58_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG0264998.1"
                     /translation="MSLYAIGDLHFSTSVNKPMNIFGSNWDGHEKKIIDNWKEVVKEE
                     DMVLVLGDTSWGINLSEAKKDLDIISKLPGQKILIKGNHDYWWTTVTSLNKLYEDMRF
                     IQTNFYEYKDYAICGGRGWICPNDVKFDETDEKVYKREEHRLRLSLESARKSGHSKII
                     VITHYPPTNDKLEESLFTKLFEEYNVEKVIYGHLHGKESFKMGLKGIRNGVEYTLASC
                     DYTEFNLIKVHD"
     gene            11187..12128
                     /locus_tag="KPD58_001217"
     CDS             11187..12128
                     /locus_tag="KPD58_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0264999.1"
                     /translation="MRWILVFIFLYLIPLVVIFRNYKNFKRSCIYSSIYVVLATTIMI
                     SNIYMSGLNKIKESMYYHNYISDETYDEKYVSNFKEKESTDLKQDNKKNEEISNNKNL
                     NNKQDRNIQNKESNEEGSNDIKEKNNSDSNVSVNNKSEKQEDNTKENNKYTSKEKNSE
                     DIKKIDMESIGIFKKEIYNIERAALVPMRECIPYTKDIAKNITKLSSIKKDVTNARNK
                     CKEVVKEYENMQIPELSKEKYTETLSNARDDVKKAYELREKSMESALKLINTKSPKYI
                     GKITEYLDLSDKQIESFKNRLNDLKEEINNSETNNIN"
     gene            12165..13322
                     /locus_tag="KPD58_001218"
     CDS             12165..13322
                     /locus_tag="KPD58_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBG0265000.1"
                     /translation="MNKQSIGKLNMFFMGITQRNLDNRDFFIELEVIFKSGRKEFKGI
                     AIEEDNKYKFNFKGKSDLYTFNELLKNISKEAENYDGLVFKYVERGTIVVIEGDNKKV
                     NVKYLDNKEEVPKIDEFTASQIKNRDYYVKVGQANALLKEIGVLTKDGKIKNDKIRKY
                     NQIDHFVELIDSILKEIKDKDCITILDCACGKSYLSFVLNFYIKEVLKKNCYFIGIDY
                     SDVVIEASKNMAKNLGYKNMSFIKEDLTNYTPNRDVDLVISLHACDTATDMAIGLGIR
                     AKSEAIVVVPCCHKELLGQYRYEAMEPILKHGVFKARFADLITDGLRTLLLEGNGYDT
                     SVVEYISPLDTPKNLMIRAIKTKTNNDKALKEYKELKSQFGVEPTLEKLIY"
     gene            13474..14034
                     /locus_tag="KPD58_001219"
     CDS             13474..14034
                     /locus_tag="KPD58_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBG0265001.1"
                     /translation="MNKLNICIDIDGTITSPYHFLPYLNDIFNKNITEAECITHNWEE
                     LYGSGIRDVYAEFNNKYIHSYDEAKIVEGATEVISTLSKGHNLSFVTARHECLTDVTK
                     NWLSRQGFSDIDVYLLGSDYKVEKARELSCDIFIEDNPLNSVQLADDGVKVILLDTNY
                     NKDVKHQNIVRVSNWIDIEKIIKQGI"
     gene            14062..14553
                     /locus_tag="KPD58_001220"
     CDS             14062..14553
                     /locus_tag="KPD58_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265002.1"
                     /translation="MIDLKIENRKGKIHVKSSEIKDILELRPDFEYVQDISNTINQEN
                     ILVFDCQLTGNSFDMDDLDIEEILEELGEEIDESYFSVLFEDVRAYLKDATDEIEADL
                     QDNYLFDNLRCYFDIYNINQEFTDFKFVFLVSFKDIKISSLANLAKIVSKRQLIGASK
                     FYS"
     gene            14782..15333
                     /locus_tag="KPD58_001221"
     CDS             14782..15333
                     /locus_tag="KPD58_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0265003.1"
                     /translation="MQSLNEIIAENLKKIRKEKHLSLDKIAQLSGVSKSMLSQIEKCE
                     VNPTISTLKKITNGLKISFTSLMERQESDIELIQKSDIDHFIEDNGKYISYPIFPFDS
                     KRRFEIFMIEIEEGGNLDSNAELPGTQEFITVFSGEVTIKINGEDYIVSSGNSIRFKA
                     DVSHIYRNSGKGIAKMSMVVYYI"
     gene            15419..16351
                     /locus_tag="KPD58_001222"
     CDS             15419..16351
                     /locus_tag="KPD58_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HBG0265004.1"
                     /translation="MNNKKAVFFFCSDLKKDPVASNVIKCCEEIMDLNQTDIVIDDNF
                     VLEFKDKNNNLFHFVKTKDVISHNYKYYLPILNRHFRDYDFAGVVNWHEGENAPEHIL
                     TAHTIGDVPTGEFGNSNPRYFKNLINAIEDIKNENLLDEFTTLTEATHWSGTTYGEES
                     KLITEYSVPMLDIEIGSSSDSFNNSIAIQVLAKSLIRVFDCDEPLKTLLCVGGVHFEK
                     SFSDIIKNKEYNISIGHVLPNQWIVSGMYDDESGFEKLEKCINSIEGGIDCIVFHDKL
                     KGTYKEQCRKLGEKLNVPVFKHKILKNPKDLPIW"
     gene            16406..17269
                     /locus_tag="KPD58_001223"
     CDS             16406..17269
                     /locus_tag="KPD58_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBG0265005.1"
                     /translation="MILSYFALLIRVVLLAFERVVVRLLGDEEGDIYKNIASSFLFFF
                     IGGVCLLPFSIMDRVSDWSFLIPCYISSLVYSIGSVAYVTSLATGEVSLVTPINSLNS
                     LFLLLLSVIFLGEGLSLAKVAGIIIMIGGVFILKKVSSFLKNVSVIFNNLPCRLMFLY
                     IFLQSVGRVLDKAFFVQVSPILYSTILYFFVGLNLFIFLAFKKKQKIICEIFTNKKGL
                     SILSGAINGYSYLALLIALNNLELSIAEPFSQVSMIITLILAHFIFKENIKEKIPGSI
                     LILIGGWLLLL"
     gene            17516..19378
                     /locus_tag="KPD58_001224"
     CDS             17516..19378
                     /locus_tag="KPD58_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG0265006.1"
                     /translation="MNYKKTAIDIIEQVGGSKNILALEHCSTRLRFKLIDRSKVNQDA
                     LKKVQGVMSIVNGAQFQVVIGNSVVDVYDEILKVCPLDTNDSTVKTEEKINLGTRFIE
                     FIINIFQPLIFAIAGAGILKSLLMLLAMFNILKSDSTIYTLLVAISDATFYFLPLMVA
                     VTTANVLKCNRLVAIAAVGYLLLPATTTALSEGVELFGFTIPNIAYNAQVFPAILCVS
                     FLATMEKIFNKYSPKPIRTFFVPMMSLAITVPITLTILGPLGYNVGTIFTTIILFLYN
                     KMGFLVVGLLAAFLPFMVATGMHKALIPYSINTIGKLGYEALYMTSSLAHNISESGAC
                     FAVALRTKDETLKQTALSAGISALMGITEPALYGITLQHKRAMLGVVFSSAISGTFLG
                     LFAVKGFVIVGPGLASMSMFIDPNNGNNLIFASIGFVIAILGSFIITLIIWKEDTNTV
                     ESSIGDVVEEELEEEKEVNILVTPVEGKVIDLSKVNDELFASKTLGDGVAIIPTNGNL
                     YAPCDSEVVMLFETKHAIGLRTKNGAEILIHIGINTVSMNGDGFKTFVKTGDNVKEGD
                     LLIQFDLDKISHANLDSTVMIVNNNGADYAYKVLNQSYGNVKKGSILFDVKGGI"
     gene            19381..20847
                     /locus_tag="KPD58_001225"
     CDS             19381..20847
                     /locus_tag="KPD58_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBG0265007.1"
                     /translation="MRKFPEGFLWGGATAANQFEGGWNLGGKGWSVSDVAKAHFDADV
                     KDYKSNNEITTKDIEEGLAHPEDEVNYPKRHGSDFYHHYKEDIALMAEMGFKTYRMSI
                     AWSRIFPNGDDKEPNEEGLQFYDDVFDELISYGIEPLVTMSHYEPPLNIVLNYDGWYS
                     RQVINMFVRYVETICERYKNKVKYWLTFNEVDSMIRHPYTTGGLVRDRFKDKNFEEVI
                     FQAMHHQFVASAIATKICHEIIPNSKVGCMLTKLTYYPYTCRPEDVLATQQKMRSIYA
                     YSDTQVFGEYPVYLLSYFKNNNIQIVKEEHDDEIMKKYPVDFISFSYYMSSCEAADTT
                     GLDITPGNTLLAVKNPYLEMSEWGWQIDSIGLRISLIELYDRYRKPLFIVENGLGAKD
                     ILTEDKKVHDQYRINYLKEHFKCMLDAIIEDGVELWGYTSWGCIDLVSESTKQMSKRY
                     GYIYVDADDYGKGTYNRYKKDSFYWYKKVIENNSIDFK"
     gene            20871..21707
                     /locus_tag="KPD58_001226"
     CDS             20871..21707
                     /locus_tag="KPD58_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG0265008.1"
                     /translation="MNYIIKKVLNSSVVLVHDAKYNEFILLGKGIGYGKKTGEYICGS
                     NDNQMFVPVENTKSKQFLELMDDIPVDILKITQEIIVEAEKLLNSSFNKNLYLILADH
                     FNFAIERMRKGIKITNRVFWEIKNYYPNEFKVGMVAIYMVEERLGIQLPEEEAANIAF
                     HFANAMASDGNSYDTIKYAKVIGEIINIFVFSLNRTLDKKSMHYMRFITHIKFFVERF
                     FSDSMLSSGDDLLFNQMKRSNPKEMLIALKVRDFLEKKYGKKLTNEEIAFLVVHIARV
                     KQ"
BASE COUNT         8269 a         2470 c         3583 g         7580 t
ORIGIN      
        1 atattatact ttttttggtt ttaattattt taagttttta tacaggcttt aaagttataa
       61 gattgataaa taataataat gaacaggaat ataaattaca aaaactagat agtaaaataa
      121 atcataagga taatataatt atcaaattaa gaagtcaaaa acatgactat ttgaaacatt
      181 tgcaagtagt gtattctcta ttaaatactg ggctagatga agatgccaaa gaatatataa
      241 tgaatatatc aaatagcttt aaatcaaaaa attcaaggta tggaaaagta tcttatttag
      301 atgctatagc ttcattaaaa tacgaggaat gtcttgaaaa ggatatatct tttgatgtct
      361 acattcaaga ttttatggat aatttagaga ttgaacctaa tgatatgagt tcagtactat
      421 taaacataat agataatgca atagagtctt tgtctactat aaaaaaagaa aagaagtata
      481 taagattaca tgcatatgaa gatgaatttc agtatattat agctataaaa aataatggtc
      541 ctaaaataca taatgttgaa agtatatttt ggaatggatt tagtactaaa gaaggacaag
      601 ataggggata tgggctacat ttagttaaaa atatattaga taagtatgat tatagtatac
      661 ttgtaaatag tgatgatttt ttaactgaat ttgtaatctt aatcccaaaa tattattagt
      721 taggaggaga aaagtctatg ataaaagttt tgatttgtga tgatgataag attgtaagag
      781 atgatatagc aaatatagct aaagagtctg gatatgtaga ttatgttaaa aaagttaagg
      841 atggtataga ggcaattgaa tttataaaaa atgagaaaat agatttattg attattgaca
      901 ttgatatgcc atataaaaat gggattgatt ctgctgaaga aattctttct atagataagg
      961 atatacatat agtatttgtg acgggtttcg ctgattatag tcttgaaagc tttaaagtgc
     1021 atcctataga ttttgtagtt aaaccattta aaaaagaaaa aatacttgat tcaattaaca
     1081 tagcaataga ccatattaac tcccataaga ttgctaataa taattttata gaagacaccc
     1141 tttttgtata taaaataagg aagcagatac atatgattaa ttttgatgat atagttatgt
     1201 ttgagaaaaa ttcaagagca attaatctac acactaaaga tgaagatata ataaagtttt
     1261 atgagaactt tgatgattta aaaaaacgcc tacctcctaa tttctttatg acccatagac
     1321 aatacatagt aaatctaaga tttatacata aggttatacc tataaataaa tcttcattag
     1381 agataaggtt tataaatttt caagaaaatg ccatattaaa caaatctcta gaaaaagatt
     1441 ttttatatag attttatagg gttaaaagat tctagtgaag atagaagaaa aatacttttt
     1501 gtgtgatata ataagtaccg taaagataat gtataagaga agtttatata aaaattattt
     1561 gtaattatga tttaatctat ataaattaca gtatagtata tattggactt tctaattttt
     1621 ttaagaaagt ccaatatatt ttagagatag tttttatact tataaattac ttaagatact
     1681 agggtcttta atcttactat cttcagcaag taatagtatc ttagacataa tctcagcagt
     1741 tttagggtct tcatcagcga atggaaggaa tatcttacct ctactttgag aatgtatagg
     1801 aagtatattc aaagcaccca tccctttttt atgtacaata ccagaaccca tatgcacaga
     1861 atactcacct aaattacctg ctataagagc atgagagcct tcaaaattga cattgttaat
     1921 ttttaataat aataaaagtt ctttagcaat tgctatacgc atctctacag tagaatggct
     1981 agtttcaggg tcaacaccac cagcatgagc gacacttaca acaaggtcta tatcacgcat
     2041 aacttctgag aatagaattg gaggtataga agctaattct aaacttttat aagtttttct
     2101 atcaaaaaat tctactgttt caatagtagg aggctctata tctgctggtg aaaaccaatc
     2161 tgcaagtgca aacatctttg ccactatatt ttctttataa aatactttct gtaatccctc
     2221 ttcataatcg acagtccaaa gctgatttct aagaagtgct acagttttct ttggctgaat
     2281 ttggtgacca gcatatctgc gagatatagt tttttcagat aattcatcac tgtttattct
     2341 ataatactca cggaatactt gcttaaatgg ttgaacaatt tgcttactaa ataaatcatg
     2401 ttggaactca ctccacttac cagattcaaa caaatgaact ggatgtgcaa gttttaattt
     2461 attttttgcc gtcaacttat aagtttcacc ttctatattg caaataaaga acttagactt
     2521 atcattataa acatatccaa tattatcatc acatacccat actagatttt taatcaaagg
     2581 gcagataact ggatttttat agatattggc aatttcttta actgtaaata ctatttcatt
     2641 tttcatagct tgttctaaac tctgttttgc acgagaatac tgactcttta aatctttttg
     2701 gacagattta agttcaagta catatggatg tttagcaata gcttttggta tactctttaa
     2761 agacttgttg cctttttgaa ctttagtaga tgcttttccg ttttcatcaa catctatcca
     2821 aattgaagta ccatcaatat cttttggttt aaatagatga gcaacttcat ttagtttttc
     2881 agtctcaaga gcccacatca tacgatttac atctccaaat ccagcacttc tagctagatt
     2941 ttctagcgct atagtacaag cttttgattc actttcacga cgttgtgcac caaattgctt
     3001 gctttcttta gcaaactttt gaataaattc ataacggaaa gttgcttctt ttaggtctcc
     3061 ttttttcata ggaagtaaag agaaacttct caatttatct tgatttctat ttgtttcaat
     3121 ttctttcatc aacatgtctt tatcaagctt accaagtgta gcatcagcat agagttgtgc
     3181 acgtctatga gagttaccac cactagttat atatttagct gacttgtaaa gtatattgaa
     3241 tctttcttcc ccaatagttt tatatgaatc taaaaaccaa tctacatcaa atgcaccatc
     3301 aatgaaacta cgtggtgaaa taggggagaa tagagcaacc tctgattctt tttgtgcaga
     3361 gaagccttca tttatatgtg catggaagta ccaaattgta cttttcagac ccttccagtt
     3421 taaatatttt tcaattatgt ttacccattg aggggcatac atagcagatt ctatcaatct
     3481 taactcagta atgtcggtat ttttaagtaa actttttaat ttttttgaat catcttcttt
     3541 agtaggatag caatttttaa gtagagaact taaaacatct ttttttgaat aattgctacc
     3601 atatgaatat cctctctgga aagtttcttt accaagagcg accaaaatat ttacaaaata
     3661 atttatacct tcaaagtatt gtatattatt agctagatta gacacttctg ttggcatatc
     3721 acctctacgt gattcaattt ctataattcg gtttgtaaca gtttttaaat tatctttaag
     3781 ccaagggtac ttttctaatg tgtcgccagc ccatctagga tttgtcaatg ttcttattat
     3841 atttgatgcg ttgtatccac ttatcatttc tctattaact tgctcttcag tcattaatcc
     3901 taattcatgt gcacgagcaa acacatttat gctagtatag aaaggaaact tatattctag
     3961 caactgataa tttgaaaacg tagtgtagaa aaacttagta aaagaaatat catcataaat
     4021 aaacgaatgt gctttatcaa gccaatagtt taaaataggg gcatttaaag cagggcagta
     4081 ttcattttgt ctgtaatagt ttttagtaaa ttcaactctt tgttttttac attgttctat
     4141 agtcaataag tttctaagtt tatagtatat ttctaaacaa tagttaaata tttctttatg
     4201 gtcaaactca tttagtaaaa ctcttaaaag ttttcttgta agtccattaa atttaagttt
     4261 ttcaagtttt gtatatattt tattttgttt tttagagtca aggacatttg aatatataga
     4321 tgaaaaccaa cttttttctg tttcattata tccacatgta cttatactat aaaataagat
     4381 atcaagtatt aatttgtaat ctaagtcaag ttttttacta gtttctctcc aaagctctgc
     4441 taatggataa ttatcaagag tatcattaga aactttatat ttaataggag tcaagttata
     4501 agtagaataa cctaaaagta ctttgtctac attacctgac catctttcaa cttcatattc
     4561 ataatctgca ttttcagtaa taagttcatc aaatttttca taaactttta ttattttatt
     4621 tatatccgta tcgagaattt tttttgcttc tttttttgtt gctacagaaa cagaatcctc
     4681 acgagtagat aaacttatat ttgagataat tttattaagc tttgatgagc tattagaatc
     4741 atctttaaaa tcaaattgtt ctggaatttg aactttgttt tcagggttat atattccaaa
     4801 accattttct ttgcagtaat cattttttgc tttacctaac aaattatcaa ttagtatttg
     4861 aacttgagat gtattctttt tagatttatc aatatcctct ataagtaaaa taagcttatt
     4921 atatagttct tgtctagcag tattgtcttt taataagttt acaatttcaa gacctccaag
     4981 gcatttttga gaatttgaag attttatcag tgaagaagca atattttcca aatttgaatc
     5041 atcttgttca agtaaaattt ttattactgt ttgtctaata gagcttgtct ttaaagacaa
     5101 tagattggtt atcttatcta attctacatc tgttagctca agctttctaa gtagactcaa
     5161 taccgtttct ctattggaaa cactcttatc acttaaacat tccaataaaa attcacgttg
     5221 tctatctgtc tttggttcat ttacaaaatt gactataagt gaagctctta aatctgaact
     5281 cataagatga caattttcca taagcacatc tataaattga gcattcatat cgtatgctgt
     5341 taaagtcatc atacatttaa gtatttcatg agaagaaatt tcaatccaag tccatggaaa
     5401 tacacttcct gaaaatgatt ttgtctttga atttaaaatt tctaaagtat gttttaaact
     5461 ttcaaattgt tcttttctag catcaaagtc tttgtcaata ggagtgtttg acaagtcaaa
     5521 attagtttct ctgttttgcc aactatagta gtagccaaca gaaccttcta catagaaatt
     5581 tgtaattacc catgctagta tctctaaatc atcaccctta atgtttttag atgctataga
     5641 atgtttaaaa tatgtacttt ctgtatgcga taaaaagtaa agagctacaa gttttttata
     5701 tttttcctga caagaaagta agttttcaat aagagagtaa gagtcatata tgttgtaagt
     5761 tgcagtagcc cataaggcaa aataaatttc aagattatca ttgctattaa ttgattctat
     5821 acggaacttt tcatcagtca atgatttata tgaaagttct aaacatttac gtattattcc
     5881 aggtttttgg tctgaaaaac caagtcctgt ccatgtatcc aaggctctta taacagaact
     5941 aaatcgctct aaattgttgt ccaaaatagt ttttaacatg tatattgatg cagaaattgt
     6001 accctcatct aaactctcta caatttgctg tctaagtcct tcttgttgtt tggctgccaa
     6061 aagaagttta gaaagcattt catagcattt atcactatga ctcttcatga taccaattat
     6121 catttgtctt gtaagtagag cagtttgatt ttctccaaat attacttctt ctaaagcact
     6181 tattactttt tgatttcctt tgtctatttc tagtgctatt ttatcactca ttatagagtc
     6241 atagtacaag accttatttg gaagagtaag ataatctata actgaaaaat cataataaat
     6301 taaatcaaca tagtttgaaa taaaccttat aatacctcta aaataatcca caagtttatt
     6361 tgagcgatac cctttacgat aatatcctcc tgaatatgta taatccatct ttcttttaat
     6421 taattggcaa agttcttcta cttgttcaga agtaaatatt aattctaatg cattttttaa
     6481 aggtccagaa aggagctcgt ccatattttt aatttcgtag tagtggcaat tgtagtattt
     6541 ctttatcata tttaagaatt tgtcttcagg ttctctataa tagtatgaac tattttctat
     6601 gcagtcccaa agagactttg ctaagtcgta gtactgtttt gaagaagatt tagtaaaatt
     6661 atttttaaaa ttttcaatta ttttaggatt tttttctact ctagtacgtt ctaacattta
     6721 tgtacctcca aattatttat tttaaaacat tcttccagca agcaaggtaa gtcttataaa
     6781 agtaaatata tctccacttt tcagatttat agaggaatcg agtttttcaa taacatcctc
     6841 atttataagc actttgtaga ttccatcttc aaatgcctgt aacgctacct ctattgcctt
     6901 ttctacagat tgtttattct cattgtaaat agagccaaat ccaacttttc caatatcagc
     6961 ttgagattca atttcttcat tagttaagaa gtttattatt gatttttctg ggattttctc
     7021 attatattca agaacatttt tagatacaat ttcagagata gcttctttta ggcaattgat
     7081 gttgtcagat acatcaaaag gggtaagttc tagcataggt ttgcgtttgc caacagcctt
     7141 aactctaaaa taaatttgca taaaatcacc tcgcaaaata ttaattatgt atttagaaat
     7201 ttaaaaattt atctatttgt tatattatat cattttgtga attattaatg tgaattatgg
     7261 aattatttta caaaaaatag aaaaaaagct atcttaaagt ctaaaaaagc aataaaagat
     7321 aacttttaac tataattata tagaattata actgtacatt tattaaattt taattttaga
     7381 attatttttg ataactatct ttttttattt ataaattttg ctcctaaata ttttttgata
     7441 tcttcttcag gagaaaagtc atcaataagt atttctttcc cagtgtcttc aaatactaca
     7501 agaacttcac agtcaccttg taagaaaaat tttctaactg tatcttcttt aacttctcta
     7561 acttttatta cattcttttt ttctttttca aagtcttttg tgtaataact tcccatcata
     7621 ttaattattt ttgtatatct attcataaaa tacctccata tgcgattaaa tttaaaaaat
     7681 atgttataat aattgtttgt attaacttaa taattataac aaggagtaat aataatgact
     7741 agtataaaaa ataaagatat tttaaattgt atagattatt ccattaaaaa taagctcttt
     7801 gacaaactaa atgatgtata taagtccttg ccagtaggaa attgttctgg atgtggaaat
     7861 tgttgtatgg agtcagtagg tataaattta attgagttct tgaatatatt ttgttatctt
     7921 gaagatagag ttgatttaaa aaaaagctgt ataagtaaaa tattagatta ttattttgag
     7981 gagtatacaa gaaagaaccc atgtccattt aaagatacta ataatagatg tttaatatat
     8041 gaagttagac cattaaattg taggatgttt ggacattgga aaaaagagga ttataataaa
     8101 aatttagata atgttattga gaaaaataat aactatagag agttaatgaa aaagcaatat
     8161 ggatttgaaa taaatgatga agtagtaaat tacaggatag attattgtga tagatttatt
     8221 ccaaataaag attatttaag taaatctaag agattaagct tttttgatga gcttatgata
     8281 ttagactcaa atatttattc aagtggaaat atagatatag actttaggga tagaggtata
     8341 gttgaatatt ttatagagtc attactttat agaaatttag catataatgt gaaaataagg
     8401 atatctaaag aatacaaaat aagaaaaaga actatagata gaataaaaag gattgttctt
     8461 gtatagtttt atattagtaa gtaataaatt gtatcataaa tattgtcaaa atgaggtgaa
     8521 gaaggtgaat cttacaaaag taaaaggaaa tacctttttt ataaaaggtg gcacgaatac
     8581 aggaatatat gtatttgaag ataacacagc acttatgata gacccagggt tatgtggttc
     8641 aagacctagg agagtaaata aagtattaga tgaaaataat atacaggtta gatatattat
     8701 aaatactcat ggtcatgatg accactatgg agcttgtaat caatttaaag aatattataa
     8761 agatgtgtgt ataatgtcat ctgaatacga taaattatat atagaaaatc cagaattgtt
     8821 ttcaaagtat attgttgggg gaaagagcaa tgtatttatg gataatatct taaaaaataa
     8881 aatgttggat gatataaaaa tagataaatc tatagaagct ggaaaaattg aattaaacaa
     8941 tgaaatgttt gatataattg aatttcaggg tcatacacct ggtagtatag ctgttttgac
     9001 gaaggataat gtgatttttg ttggcgattt attgatagga gatgaaattc tatcaaaata
     9061 cgatttcttg tttctatatg atatacaaga acaaataaat tcattagaaa aattaaaaaa
     9121 tatagatttt gagtacttag ttttagggca tggcaagcaa gttatatcga aagaagattc
     9181 ttataaatta atagaaaaac atttgaatgc tttaaaaaaa tatttatatc aaataagaga
     9241 gttgcttgta gaaccgaaat ctttagaaat tttattaaaa aacattataa ataacaataa
     9301 tttaagcaat aattataaag agtatcattt ttttaaatct tcgttagtat ctgtcataag
     9361 ttacttagtt gatttagaag aaatagggta tatattggaa aatggagaat tgctttacta
     9421 tacaaaaaca aaataaatta tgataaaata aagaaggtca taaaaaacaa ataaagggtg
     9481 agcaatatgg aatacgaaaa atggaatgag atactagcac cttatgaaca tgcagtagag
     9541 gaattgaaaa taaaatttaa aaatataaga aaagaatatt tagcaaaagg tgaatattct
     9601 cccatagagt ttgtcacagg tagaacaaaa aaaatatctt ccataatatc taagttaaaa
     9661 aggataaatg caaaagatat agaaactgaa attgatgata ttgctggtat aagaataatg
     9721 tgccagtttg ttgaggatat atatgctata gttgatttga taaaagtaag aaatgatatg
     9781 actataatag gagaaaaaga ttatattaca aattacaaag atagtggtta tagaagctat
     9841 catgttataa taaaataccc tataaactcc attgcgggtt caaaggaaat tatatgtgaa
     9901 atacaaataa gaactcttgc aatgaatttc tgggctacaa tagaacattc attaaaatat
     9961 aaatatgacc attacatacc tgagactctt gctgaaaggc ttagaagagc atctgatgct
    10021 gcctttcttt tagaccaaga aatgagtgaa ataagagagg acataatgaa agctcaggct
    10081 atgtaccaaa tgaagtctat agctattaga gatactttaa atagaataca agagttatat
    10141 gacttgggtg atacacataa agctatgcag taccaaagaa aacttgatag aactgaaaca
    10201 gataaaaata taactgaaat tttggaatta aaacaagaaa tagatatatt attagagcaa
    10261 tataaggata ctaaggtaga tgataaagta gttcagtaaa aactacttgt agaaaatttg
    10321 ctaaatatta atggggctgc ctcttaatgg aaaataattt ctaaaaagag acagcctatt
    10381 tagtgtaatt aagaaaggaa tgatattatg agtttgtatg ctataggtga tttacatttt
    10441 tcaacatcag tcaataagcc aatgaatatc tttggaagta attgggatgg tcatgaaaag
    10501 aaaattatag ataattggaa agaagttgtt aaggaagaag atatggtctt agtactagga
    10561 gatacatctt ggggaattaa tttatccgaa gctaaaaaag atttagatat aataagcaaa
    10621 cttccaggtc agaaaatact tataaaggga aatcatgatt actggtggac aaccgttaca
    10681 agtttaaata agctatatga agacatgcgt tttattcaga caaattttta tgaatataaa
    10741 gattatgcaa tttgtggagg taggggctgg atatgtccta atgatgttaa atttgatgag
    10801 actgatgaaa aagtttataa aagagaagaa catagactta gactatcatt agaatctgct
    10861 cgtaaaagtg gtcactcaaa aataattgtg attactcatt atcctcctac gaatgataaa
    10921 ttggaagaat ctttatttac aaagttattt gaagaatata atgttgaaaa ggtgatttat
    10981 ggtcacttgc atggtaaaga atcttttaaa atgggattga aggggattcg aaatggagta
    11041 gaatatacat tagcatcttg tgactataca gaatttaatt taataaaagt tcacgattaa
    11101 aaaacataaa ttggtaaata ctataagata catgattaaa aataaatatt tgagggattt
    11161 cggttttgag aaaggagaca cgacttatga ggtggatatt agtttttata tttttatatc
    11221 ttattccttt agtagtgata ttcagaaatt acaagaattt taagaggtct tgtatctaca
    11281 gtagtattta tgtggtatta gctacaacaa taatgataag taatatttat atgagtggac
    11341 ttaataaaat aaaagagtct atgtattatc acaattatat ttctgatgag acatatgatg
    11401 aaaagtatgt atcaaatttt aaggaaaaag aaagtactga tttaaaacag gataataaaa
    11461 agaatgaaga aatatcaaat aataaaaatt taaacaacaa acaagataga aatatacaaa
    11521 ataaagaaag taatgaagaa ggaagtaatg atataaaaga aaagaataat tctgatagta
    11581 atgtttcagt gaataataaa tcagaaaaac aagaagataa tacaaaagaa aataataaat
    11641 atacaagtaa agaaaaaaat agtgaagata taaaaaaaat agatatggaa agtataggaa
    11701 tttttaagaa agaaatttat aatatagaaa gagcagcttt agttccaatg agagaatgta
    11761 taccttacac taaggatata gcaaaaaata taactaaatt atctagtata aaaaaagatg
    11821 ttacaaatgc cagaaacaag tgtaaagaag ttgtaaaaga atatgaaaat atgcagattc
    11881 cagaactttc taaggagaag tatacagaaa cattgagtaa tgctagagat gacgtaaaga
    11941 aagcatatga gcttagagaa aaatctatgg aaagtgcttt gaaattaata aatactaaaa
    12001 gccctaaata tatagggaag ataacagaat atcttgattt atcagataag cagatagaaa
    12061 gttttaaaaa tagattgaac gatttaaaag aagaaataaa taattcagaa acaaataata
    12121 ttaattaata agatttaaag atataagtta aaagggagat tatgatgaat aaacagagta
    12181 taggaaaact aaatatgttt tttatgggga ttactcaaag aaaccttgat aatagggatt
    12241 tttttattga gttagaggtg atttttaaat ctggaagaaa agagtttaaa ggaattgcaa
    12301 tagaggaaga taataaatat aaatttaatt ttaaaggaaa atcagattta tatacgttta
    12361 atgagttgct taagaatatt tctaaagagg cagaaaatta tgatggatta gtatttaaat
    12421 atgttgagcg aggaactatt gttgtaattg aaggagacaa taagaaggta aatgtaaaat
    12481 atttagataa taaggaagaa gtacctaaaa tagatgaatt tacggcatct caaataaaaa
    12541 acagagatta ttatgtaaaa gtaggacaag caaatgcact tttaaaagaa attggagtac
    12601 ttacaaaaga tggtaagata aaaaatgata aaataagaaa atacaatcag atagaccatt
    12661 ttgttgaatt gatagatagt attttaaaag aaataaagga taaagactgt ataactatct
    12721 tggattgtgc ttgtggaaaa tcttacttaa gctttgtctt aaatttttat ataaaagaag
    12781 tattgaagaa aaactgttat tttataggaa tagattattc agatgtagtt atagaagcat
    12841 caaaaaatat ggctaaaaac ttgggttata aaaatatgtc atttataaaa gaggatttga
    12901 ctaattatac acctaatagg gatgttgacc ttgtaataag tctacatgct tgtgatacag
    12961 ctacagatat ggcaataggt ctaggaatta gagcaaaatc tgaagctata gtagtagtac
    13021 catgttgtca taaggagtta ttagggcaat atagatatga agctatggaa ccaatattaa
    13081 aacatggtgt atttaaagct agatttgcag atttaataac tgatggttta agaacattgt
    13141 tattggaggg caatggatat gatacttctg tagtagaata catatctcca cttgatacac
    13201 caaaaaattt aatgataaga gcgataaaaa ctaaaacgaa taatgacaaa gcattaaaag
    13261 aatataaaga actgaaatca caatttggag tggaaccaac cttagaaaaa cttatatatt
    13321 agttaaaaat gtaaaattta cgaaaagctt gcttaaatat gcaagctttt tggtattata
    13381 taggtatagt catatccaga gttttgattt ataattaaag ctgtgctata atatttgatt
    13441 aatcaaatca ataaatgtta catggaggta aaaatgaaca aattgaatat atgtattgat
    13501 atagatggaa caataacaag cccttatcat tttcttccat acctaaatga tatctttaat
    13561 aaaaatataa ctgaagcaga gtgtataaca cataattggg aagaattgta tggttcggga
    13621 ataagagatg tgtatgcgga gtttaataat aagtatatac actcatatga tgaagcaaag
    13681 attgtagagg gtgctacaga ggtaattagc acattatcta aaggacataa tttatctttt
    13741 gtaacggcta gacatgagtg tttaactgat gtaactaaaa attggctatc gagacaagga
    13801 ttttcagata tagatgttta cttattagga agtgattata aagttgaaaa ggcaagagaa
    13861 ctgagttgtg acatatttat agaagataat cctttaaact cagtgcaact agctgatgat
    13921 ggtgttaaag taatacttct ggatactaac tataataaag atgttaaaca tcaaaatata
    13981 gttagagtta gtaattggat agatattgaa aagattataa aacaaggtat ttaaagattt
    14041 gtagattgaa agggggagca tatgatagat ttaaaaattg aaaatagaaa aggaaaaatc
    14101 catgtaaaga gcagtgaaat aaaagacatt ttagaattac gtccagactt cgaatatgta
    14161 caagatatat caaatactat aaatcaagaa aatatattgg tatttgattg ccagttgaca
    14221 ggaaattctt ttgatatgga tgatttagat atagaagaaa tattggaaga gttaggtgaa
    14281 gaaatagatg agtcttattt cagtgtttta tttgaagatg taagagcgta tttaaaagat
    14341 gctactgatg agatagaagc agacttacag gataattact tatttgataa tttgagatgt
    14401 tattttgata tatacaatat aaatcaagaa tttacagatt ttaagtttgt atttttagtt
    14461 tcttttaaag atataaaaat ttcttcatta gcaaacctag ctaagattgt ttctaagaga
    14521 cagcttatag gtgcatctaa gttttatagt taaaatgaaa tattattata ataaaagcta
    14581 gtaaccttgt aaaaatatag gttactagtt ttttatttat tcattttcac aaaatttcag
    14641 aaaactattt acattctaaa aaaagtaatg tatattaaac ataatgtaca ttataatgga
    14701 taaaaagttt ctattaaatt actaagttta tttaataatc aaaataattt atcttatatt
    14761 agtatgtatt agggggtatt tatgcaaagt ttaaatgaaa taattgctga aaatttaaaa
    14821 aagataagga aagaaaaaca tcttagttta gacaagattg cacaacttag tggtgtaagt
    14881 aagagtatgc tatctcaaat tgaaaagtgt gaggtaaatc ctactatatc tactttgaag
    14941 aaaataacta atgggctaaa aatttcattt acatcattga tggagaggca ggaaagtgac
    15001 atagaattga ttcaaaaaag tgatatagac cattttattg aagataatgg aaagtatata
    15061 agttatccaa tatttccatt tgactccaaa agaagatttg agatatttat gattgaaata
    15121 gaagaaggtg gaaatttaga ttcaaatgct gaacttccag gaacacaaga gtttataact
    15181 gtttttagtg gcgaagtaac cattaaaata aatggagaag attacattgt atctagtgga
    15241 aattctataa gatttaaagc tgatgtatca catatatata gaaactcagg caaaggtata
    15301 gctaaaatga gtatggtagt atattatata taaataattt aatggaatta gagtgatatt
    15361 tagatgattt taggtatgaa gatatattat atttaagtta taggagtgtg atttatggat
    15421 gaataataaa aaagcagttt ttttcttttg cagtgattta aagaaagacc cagtagccag
    15481 taatgtgata aaatgttgtg aagaaataat ggatttaaat caaactgata ttgttattga
    15541 tgataatttt gtattagagt ttaaggataa aaacaataac ttatttcatt ttgtaaaaac
    15601 aaaagatgta ataagtcata attataaata ttatcttcca attttaaata gacattttag
    15661 agattatgat tttgcaggag ttgtaaattg gcatgaggga gaaaatgctc cagaacatat
    15721 cctaacagct catacaattg gagatgttcc aacaggagaa tttggaaatt caaatccaag
    15781 atactttaaa aatctaatca atgctattga ggatataaaa aatgaaaatc tacttgatga
    15841 atttactaca ttaacagaag caactcactg gtctggaaca acatatggtg aagaatctaa
    15901 attaattaca gaatattctg tacctatgtt ggatattgag ataggaagta gtagtgatag
    15961 ttttaataat agtatagcaa tacaggtatt ggcaaaatca ttaattagag tatttgattg
    16021 tgatgaacct ttgaagactt tgttatgtgt tggaggggta cattttgaga aatctttctc
    16081 agatattata aaaaataaag aatataacat atcaattgga catgtattgc caaatcaatg
    16141 gattgtaagt ggaatgtatg atgatgaatc tggatttgag aaactagaaa agtgtataaa
    16201 tagcattgaa ggaggaattg attgtatagt ttttcatgat aaattaaaag gtacctataa
    16261 agagcaatgc cgtaaactag gtgaaaaact taatgtacct gtatttaaac ataaaatatt
    16321 aaaaaatcca aaagacttac ctatatggta gtcttataca gttaatataa caataagtcg
    16381 gattaatttt taagcgaggt caaaaatgat attatcttat tttgcattat taattagagt
    16441 agtgctatta gcttttgaaa gagtagttgt aagattatta ggtgatgaag aaggagatat
    16501 ttataaaaat atagcttcat catttctatt tttctttata gggggagtat gtttacttcc
    16561 atttagcata atggatagag ttagtgattg gtcgttttta ataccatgtt atataagtag
    16621 tttagtctat tcaattggtt cagtagcgta tgtaacgtct ttagcaactg gggaagtttc
    16681 acttgttact ccaataaata gtttaaattc attgttttta ctgcttttat cagttatatt
    16741 tttaggtgaa ggtttaagtt tagcaaaagt tgcaggaatc ataattatga ttggtggagt
    16801 gtttatatta aaaaaagtaa gttctttttt aaagaatgtc tctgtaattt ttaacaattt
    16861 accatgtaga ttaatgtttt tatacatatt tttgcagtca gtaggaagag tattggataa
    16921 ggcttttttt gtacaagtat ctcctatatt atattcaact atactgtatt ttttcgtagg
    16981 tttaaattta tttatctttt tagcatttaa aaagaaacaa aaaataattt gtgagatttt
    17041 tactaataaa aaggggcttt caattttaag tggtgctatt aatggatatt cttatttagc
    17101 attattaatt gctttaaata atctagaact tagtatagca gagccatttt ctcaagtatc
    17161 tatgataata actttaatac ttgctcattt tatattcaaa gaaaatataa aagaaaaaat
    17221 accaggttca attttgatac taattggtgg gtggctactg ttactttaat tgacataaaa
    17281 aatgaattat aaaggtgcta ttagttagta tcagaaaatt taaaaaagta ttgacaattc
    17341 ttttaggcaa atgttaatat aataatacaa aatagtataa atagaagaca tgttactggt
    17401 aatgcaggca ggatcgaata tattgctttg atttttcaag gtgtttatat aggttctgcc
    17461 ttttttgcat cttgaatggc cattcataaa aaattatgga gaaggagatt acaagatgaa
    17521 ttataaaaaa actgccattg acattattga acaagttggt ggttcaaaaa atattttagc
    17581 tttagaacac tgttcaacta gactacgctt taagttgatt gatagaagta aagtcaatca
    17641 agatgcttta aaaaaagtac aaggtgttat gagtatagtt aatggggcac aatttcaagt
    17701 tgtcattgga aatagtgttg ttgatgtgta tgatgaaatt ttaaaagttt gccctttaga
    17761 tacaaatgat agtactgtaa aaactgaaga aaaaataaac ttaggaacaa gatttataga
    17821 atttattatt aatatttttc aaccactgat ttttgctatt gcaggagctg gtattttaaa
    17881 atcactgttg atgttattag caatgtttaa tattttaaaa tcagattcta ctatatatac
    17941 attattagtt gctatttcag atgcaacatt ctatttccta cctcttatgg tagctgtaac
    18001 tactgccaat gtattgaaat gtaatcgttt agtagctatt gccgctgtag gatacttatt
    18061 gcttccagca acaacaactg ctttaagtga aggtgttgaa ctatttggat ttacaattcc
    18121 aaacattgcc tataatgcac aggttttccc ggctatctta tgtgtgtctt tcttggcaac
    18181 aatggagaaa atttttaaca agtattcacc aaaaccaatt cgaactttct ttgttcctat
    18241 gatgtctttg gcaattactg ttccaattac tctaactatt ttaggtcctt taggatataa
    18301 cgttggtaca atttttacaa ccattatttt attcttatat aacaaaatgg gattcttagt
    18361 tgtaggatta cttgctgctt ttcttccatt tatggttgca acaggaatgc acaaagcact
    18421 aataccttat tcaattaata caattggaaa gttgggttat gaagctttat atatgacatc
    18481 ttcattagcc cacaatattt ctgaaagtgg tgcttgtttt gcagtagcct tgcgaacaaa
    18541 agatgaaaca ttaaaacaaa cagcattatc tgcaggaatt tctgccttga tgggaattac
    18601 agagccagct ttatatggta ttaccttaca acataaaaga gcaatgttag gtgttgtatt
    18661 ctcaagtgca atttctggta cctttttagg attatttgca gtaaaaggat ttgtaattgt
    18721 tggaccaggg cttgccagta tgagtatgtt tattgaccca aacaatggaa acaatttgat
    18781 ttttgcgagt attggttttg ttattgcaat attaggctcg tttattatta cactgattat
    18841 ttggaaggaa gatacaaata cagtagagtc atcaattgga gatgttgttg aagaagagct
    18901 agaagaagaa aaagaagtaa atatcttagt aactccagta gaaggaaaag taatagattt
    18961 aagtaaggtt aatgatgaac tatttgcaag taagactttg ggagatggag ttgcaattat
    19021 accaaccaat ggaaatttat acgcaccttg tgattcagaa gttgtgatgt tatttgaaac
    19081 aaaacatgca attggtttaa gaacaaaaaa tggggctgag attttgattc atattggaat
    19141 aaatactgta agtatgaatg gagatggatt taaaacattt gttaagacag gcgataatgt
    19201 aaaagaagga gatttattaa ttcaatttga tttagacaaa atctcacatg ctaatttaga
    19261 ttcaactgtt atgattgtaa acaataatgg agcagattat gcttataaag tgttaaacca
    19321 gtcttatggc aatgtaaaaa aaggaagtat cttgtttgat gtaaagggag gaatataaat
    19381 atgagaaaat ttccagaagg atttctttgg ggaggagcta cagctgctaa tcaatttgaa
    19441 ggtggttgga atttaggtgg gaaagggtgg tctgtatcag atgtagcaaa ggcacacttt
    19501 gatgcagatg tgaaagatta taaatctaat aatgaaatca caacaaaaga tatagaagaa
    19561 ggattagcac atcctgaaga tgaagtaaat tatccaaaac gtcatggtag tgatttttat
    19621 catcactata aagaagatat tgctctaatg gcagaaatgg gatttaagac atatcgaatg
    19681 agtattgctt ggtctcgtat ttttccaaat ggagatgata aagagccaaa tgaagaaggt
    19741 ttacagtttt atgatgatgt atttgatgag ttgataagtt atggaattga accacttgtt
    19801 actatgtcac attatgaacc accattaaat atcgttttaa actatgatgg atggtattca
    19861 cgacaagtta tcaatatgtt tgttaggtat gtagaaacta tctgtgaaag atataaaaac
    19921 aaagtgaaat attggcttac atttaatgaa gttgattcta tgattcgtca tccgtacact
    19981 actggtggtt tagttcgaga ccgttttaag gataaaaact ttgaggaagt tatttttcaa
    20041 gctatgcatc atcaatttgt agctagtgcc attgcaacaa aaatttgcca tgagattatt
    20101 ccaaattcaa aagtagggtg tatgttaaca aaattaacgt attatccata tacatgtaga
    20161 ccagaagatg ttttggcaac tcaacaaaaa atgagaagta tttatgcata tagtgatacg
    20221 caggtttttg gtgagtatcc agtatattta ctgtcgtatt ttaaaaataa taacattcaa
    20281 atagtaaaag aagaacatga tgatgaaatt atgaaaaagt atccagtaga ttttatttca
    20341 ttctcttact atatgtcttc ttgtgaggca gctgatacaa ctggattaga tattacacct
    20401 gggaatacgt tattagctgt aaaaaatcct tatttagaaa tgagtgaatg gggttggcaa
    20461 attgattcaa taggtctacg tatatctttg attgaattat atgatagata cagaaagcct
    20521 ttatttatag ttgaaaatgg attgggagct aaagatattt taactgaaga taaaaaagtt
    20581 catgaccaat atagaattaa ttatttaaaa gaacacttta aatgtatgtt agatgcgatt
    20641 atcgaagatg gcgttgaact ttggggatat acttcatggg gttgtattga tttggtcagt
    20701 gaatcaacga aacaaatgtc aaaacgttat ggatatattt atgtggatgc tgatgactat
    20761 ggaaagggga catataatcg atataagaaa gatagttttt attggtataa aaaggtaatt
    20821 gaaaataata gtatagattt taaatgacca atagaaggtg agcaaataaa atgaactaca
    20881 taataaagaa agttttgaac tcgagcgttg ttttagttca tgatgcaaag tataatgaat
    20941 ttattttgct agggaaagga attggatatg gtaaaaaaac aggagaatat atttgtggtt
    21001 ccaatgataa tcaaatgttt gtccctgttg aaaatacaaa atcaaaacaa tttttagaat
    21061 taatggatga tattcctgtc gacattttaa agataacaca ggaaataata gtagaggcag
    21121 aaaagttatt gaactcatct tttaataaaa acttatactt aatattagca gaccatttta
    21181 actttgcaat tgaaaggatg cgtaaaggaa taaaaattac aaatcgagta ttttgggaaa
    21241 taaaaaatta ttatccaaat gagtttaagg ttggaatggt tgcaatttat atggtagagg
    21301 agagattggg cattcaatta ccagaggaag aagcagctaa cattgctttc cattttgcaa
    21361 atgctatggc ttcagatgga aattcatatg atacgattaa gtatgccaaa gtaattggag
    21421 aaataattaa tatttttgtt ttttctttga atagaacctt agataaaaag agtatgcatt
    21481 atatgagatt tataacacat ataaaatttt ttgtggaaag atttttttca gatagtatgt
    21541 taagcagtgg tgatgattta ttatttaatc aaatgaaaag gtcaaatcca aaagaaatgt
    21601 taattgcatt aaaagttaga gattttttag aaaagaaata tggaaaaaag ttaacaaatg
    21661 aggaaatagc tttcttggtt gtacatatcg cacgagttaa gcaatagagt gaattactaa
    21721 tattatctta tacgtctaca tggtagaaaa ttgaaagaag gtaaagtata taaagaaact
    21781 gagacatatt attttgttgt tcattatgtt tattttatga gtgtatcctg ttaaaaattt
    21841 aagttaatat ttataattaa aatatacaaa tagttaaaat aagtttatgt ggttatgaat
    21901 tt
//
LOCUS       DAELLV010000038        21818 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.038, whole genome shotgun sequence.
ACCESSION   DAELLV010000038 DAELLV010000000
VERSION     DAELLV010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21818
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.038"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            26..472
                     /locus_tag="KPD58_001227"
     CDS             26..472
                     /locus_tag="KPD58_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG0265009.1"
                     /translation="MDFKNLQYESLSYIICNLQKNFKLYCEKCLKPYKLTNGLYFYLI
                     YINKNRNCSLNDVSTEFEVDKAHTTRTISRLEQDGYIEKIQNPNDSRAFQLRVTDKGE
                     EVLGDIKNIFSKWDNHIKKEFSDTEYKELVKNLHVVKDIKTAVEEE"
     gene            474..1568
                     /locus_tag="KPD58_001228"
     CDS             474..1568
                     /locus_tag="KPD58_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HBG0265010.1"
                     /translation="MYCNLLKPIKIGNLELKNRVSFAPTSMGLKLEEKIKKFSDIAKS
                     GVALITLGDVSIRPSFHKVAISLSDEDGVIKYKKIVDEIHNSGAKVSAQLFCSDYDVN
                     LIKDTMNMGITSHDEIKKIMNDGVKDYITNMPKEEIKNIINLFKVTALNAKKAGFDMI
                     QIHGDRLVGSFSSSIFNNRNDEYGGTCDNRSRFASEIISSIRDEVKDIPIDYKFAIRQ
                     ENPHYGNAGVLLSEVEYFVKKFESLGVNSFHVTLANHSKLEDTIPTNNHPYFKDEGCF
                     LYLADEVKKHTNLPVCGVGKLSSPDFIESIISNNRVDMVSMSRQLLADSNWLQKVKDG
                     RVDEIKKCCYCNKKCADALQTRSQFGCILD"
     gene            1656..2186
                     /locus_tag="KPD58_001229"
     CDS             1656..2186
                     /locus_tag="KPD58_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG0265011.1"
                     /translation="MKSLVLYSSLTGNTKKIAYTIYDEIQEEKDIKDVNELVDYGIDY
                     ENYDIVFLGYWVDKGICDKNSKQVLENIHNKKIALFGTMGASKKGSYGASIIEKIESI
                     IPKDNEILGSFICQGKIAEGLKAKYKEMLKLSPDNEHIRQQLNNHEESQSHPDEQEIY
                     EASMFAKNMMIKASIV"
     gene            complement(3121..4497)
                     /locus_tag="KPD58_001230"
     CDS             complement(3121..4497)
                     /locus_tag="KPD58_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265012.1"
                     /translation="MKYKIGFLTTSYFMSDKFKQALKELNDTCEITFIELKGENPFNN
                     LPNIYYEQVDNFDGFVCSGIVPYSELIINIKDIKVPLNFLKLDERDFYKYLFKLLNSQ
                     KDIDFTKSFMDFLREDNNYYDIYSLIDSNKCPYTIKDFNIPQPVYDFKKLNDKLLEIH
                     LDLLRDNKINLSFTRNYIISCELNKLGYDCIYMVPSTESILNTFKSLVNEITLQNLDK
                     NKSATCIVTVNSSEYINEDLIFKNDEIQNQIYNTILNSLSRHGFYGVQVKKNDMKIEI
                     HTTKEMLNNMTNNYTDFFISEDLKEIKYSLNIGWGIGNTNIHAEQNARQANGKSASLN
                     GNCTFIVNDTNDTIGPLYSNKNSNNIEDSSIANKVASFIPLSNTNVSKIMCMINDRNS
                     NNVSAEILADYMNITLRSANRILSILYKAGIATIVNTKLDNQRGRPKKIYKVDFLSFL
                     NKMQKLNS"
     gene            4766..5938
                     /locus_tag="KPD58_001231"
     CDS             4766..5938
                     /locus_tag="KPD58_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG0265013.1"
                     /translation="MNTYLNEISKFVDENREEIVSLWKEIVNMESYTHCKESVNKLAK
                     RLKFEFEKEGLDCELVDVGDNGSTLIGTLGSNIDKKPIIFSGHMDTVFETGTFGENPF
                     KIIEGKAYGPGVLDMKGGIIISLYVIKALNKIGYKERPIKIVFSGDEEIGHKDSTGAD
                     VILREAKGGLCAFNMETGLVDNSLCVGRKGRIGCNIHVKGVETHAGNDFEGGRNAIEE
                     MANKILRIQKLTNLDVGTTVSVSVIKGGRVENSIPEDCSIKVDLRFEKVEEMENVKEQ
                     VREICKETYIEGTSTEVNFISEMMPFETTEDVMRFHTFVNEVSKENGFGELNAKRLGG
                     SSDASYLTIANVPTICSFGVRGEWNHTSREYAVVNSMFERVKLISTVILNLDKFEV"
     gene            5952..6599
                     /locus_tag="KPD58_001232"
     CDS             5952..6599
                     /locus_tag="KPD58_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265014.1"
                     /translation="MDVTAGDNKVTWKGYLSLFVLIVLFSGVFKDSQGFLKALDFANL
                     TGSFGTIADGVDFMGKGGTGAKDGFLFALTLIPTVCFAVGLIDVVESMGGMKAASKIF
                     NPLLRPLLGIPGIAGIAFVSSFTSSDVASIMTKELFESGEITDDERTIFVAYQYAGSA
                     VILNTINTQAPLLPIALLALGPIILIEIFCKILGANLVRLIIGVRNKKNKAKEAV"
     gene            6601..7110
                     /locus_tag="KPD58_001233"
     CDS             6601..7110
                     /locus_tag="KPD58_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBG0265015.1"
                     /translation="MSQQNTGNAIVNSKPKKTAVEIFMGGAKKGFYIGVEQILPAMIM
                     GYVIVQFLQLTGLVDILGNIFGPVMGIFGLPGESVVVLIAAFFAKAAGAATAANLFAQ
                     GLITAPQATILIMPCMLMGTLVGHYARIVLVANASTKYRMLLLAVPIFDAIVGMLIVR
                     VLLTVMGLM"
     gene            complement(7474..8586)
                     /locus_tag="KPD58_001234"
     CDS             complement(7474..8586)
                     /locus_tag="KPD58_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBG0265016.1"
                     /translation="MKISEVATPNFLLDLDQLEKNIDKIQQICTNNNKQLWPMLKTHK
                     STYIARLQKNAGASGFLVGTLDEGEALIKAGFEDIMLAYPYMGEVNINRIVTMASKIN
                     LICATDNIECAKAYSEAFSKSKIECKLLIIVDCGLHRFGVEPEKVVELATEISKLENI
                     KIVGISSHPGQVYGCSSPEGVPDVCVQEDSSMEKAYKALTDNGFNIEIVASGSTPTVE
                     SEAASKTITAVRPGNYVYYDAIQIGLGCATEDMCALTVVCTVISKNSSGYYLIDCGSK
                     CLGLDKGAHGNSSIVGYGRVCGHPELTIDSLSEEVGKIKVNGETDIKIGDRIRIIPNH
                     SCSTANLTSNLLGFRGDTIEKVIEVDIRENSKQIIL"
     gene            8884..9150
                     /locus_tag="KPD58_001235"
     CDS             8884..9150
                     /locus_tag="KPD58_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HBG0265017.1"
                     /translation="MNLEPKKLIEQIGKEKDKKDIWIVGGGDIVKLFMKDNLIDDYYI
                     YILPIILGRGISLFKSGIDKTNLNFKKASNIGELVKLEYSKKQK"
     gene            9395..11122
                     /locus_tag="KPD58_001236"
     CDS             9395..11122
                     /locus_tag="KPD58_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003496244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0265018.1"
                     /translation="MKLILSYLKNYKLLIVLNILAIFSFALVELGIPTIIAKIIDNGI
                     ANQNIAYIKQMGIVIVIISIIGVVGSILLGYCSAKVSTSVTRDIRNDIFEKSQEFSHT
                     EYNKFGISSMITRTTNDAFQIQQFVNILLRTALLTPVMFIISIIMTIRTSVELSIVLA
                     ISVPFIIIGVAIIAKVSQAISSKQQKGLDKLNLISRENLTGIRVIRAFGNDDYETERF
                     EKTNTYYANVSKKLFKLMSITQPAFFLLLNIAVLAVFWISSEKINIGELQVGQLVAFL
                     EYLFHAMFSIMLFSMVFIMYPRAEVSANRIKELLNEEPLIKNPENGIKDTENKGIIEF
                     DNVTFTYPDGEASVLKDISFTAKTGETVAFIGSTGSGKSTLINLIPRFYDVTEGSIKI
                     NGVDIREYDLKALRKKIGFIPQKSLLFTGSIANNIRFGKHKAGESELEYSAKVAQAYE
                     FISKKPRKFDELISEGGANVSGGQKQRLSIARAIIRRPEIYIFDDSFSALDFKTDAIL
                     RAKLKKETKDAIVLIVAQRISSIIDADKIIVLNEGQVVGMGTHKELLKNCEIYYEIAT
                     SQLKKEELE"
     gene            11122..12912
                     /locus_tag="KPD58_001237"
     CDS             11122..12912
                     /locus_tag="KPD58_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0265019.1"
                     /translation="MKKSKGFKKTIGRLLPFVKKYKFSFIIAIICIISAATMNALAPK
                     TEGLIITQLTKDVISIAKGVPGASVNFDYVTKILVILACIYFANAIFTYTSSFLLTNA
                     IQNTMRDLRNEVENKIRRLPISYFDSNSFGDVLSRISNDVDTISNALQQSFMQIVNSI
                     LVIILALSMMFTINIYMALIALFIIPISYFVSKFVVKKSQSRFSLQQNALGKLNGKVQ
                     EMYTGFNEIKLYGKEEDSIKEFKKVNQELCENGFKAQFISSMMNPMVSLVTYFGIAAV
                     AVVGSIYAVSGGITVGNLQAFVRYIWQINQPLSQMTQLSTVIQSSFAAIERVFEILDE
                     EEEIPDVENPVKIENVKGNVTFEHVNFGYGENSTLIEDLNAEVKSGQMVAIVGPTGAG
                     KTTLINLLMRFYDVKKGAIKIDGVDIRDMKRKDLRSMFGMVLQDTWLFNGTIFENIEY
                     GRFGATKEEIIQAAKVANVHHFITTLPDGYNMFLNEEASNISLGEKQLLTIARAFISD
                     PSILILDEATSSVDTRLELMLQKAMRNLMNGRTSFVIAHRLSTIRNADLILVMNNGSI
                     IEQGNHDELMEKGGFYEKLYNSQFADKESE"
     gene            complement(13074..13382)
                     /locus_tag="KPD58_001238"
     CDS             complement(13074..13382)
                     /locus_tag="KPD58_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265020.1"
                     /translation="MFNKIYEKKEFIVFQVKKGYVVYNTRKSFEEGHTHLKHFEAAKT
                     AIDLAINKKIPRSKDGYYLTSLIRISDDGYYIDKLSELLYVREQKGKKEKYCNSGYQM
                     "
     gene            complement(13644..14192)
                     /locus_tag="KPD58_001239"
     CDS             complement(13644..14192)
                     /locus_tag="KPD58_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265021.1"
                     /translation="MIFLNEDVVTNETIDKDYAIEEVRMACKHFGDLYFYFSKVLFEE
                     FGEDKTSEILRKVLFERSEERAIAMRERAHENGDELIADNIISTTDVPFLGWVPEFEE
                     LHCPYGASWLLRFEENPWFKKFASLYCDVTDTTVAEVFTGDTSHKITKNILWGDKSCE
                     RIYFHDDKVSEGKYTYGNKNCK"
     gene            complement(14295..14966)
                     /locus_tag="KPD58_001240"
     CDS             complement(14295..14966)
                     /locus_tag="KPD58_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0265022.1"
                     /translation="MATFKRLQEQAYDYLKELILSGKMIENEIYSETKLASEIGISRT
                     PIRDALQRLSQDGFIDIIPSKGFRIHQITANEIVEIFQIRSAIEGFCTFLITSQYKEA
                     RAVETISKLKHLLDKQKDILLGDKNLNSFAEYDTLFHTTIVSYAQNTEFDKMFNNYMY
                     RIKKLALDSLSHEGRLETTLKEHTDIFNNIANGCIEDIYRTTLIHMETPKYINLEDFC
                     NNKLF"
     gene            15362..16267
                     /gene="garR"
                     /locus_tag="KPD58_001241"
     CDS             15362..16267
                     /gene="garR"
                     /locus_tag="KPD58_001241"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HBG0265023.1"
                     /translation="MEGYKMKLGFIGLGIMGKPMAKNLLKDGCNLLVYDINKSAVDEL
                     ISCGAKYASVLEMGQECDIVFTILPNGTIVQDILFGIDGLAKTLKEGSIVVDMSSVTP
                     TESILCANKLKDMGLEFIDSPVSGGEPKAIDGTLAFMAGGKEEIYKKVEPFFNIMGSS
                     SILIGDNGSGSVTKLTNQVIVNLTIAAVSEAFVLAAKAGADPEKVYKAIRGGLAGSTI
                     LDAKIPMIMNRDFKPGGKISINLKDIKNVMQTAHNLDVPLPMTSQLLEIMQTLKVHGH
                     LEDDHSGIAQYFEKLAGVEIKKHDN"
     gene            16403..17785
                     /locus_tag="KPD58_001242"
     CDS             16403..17785
                     /locus_tag="KPD58_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBG0265024.1"
                     /translation="MITGYGLLIAFVISIGILLVSIIKFKVNPFLALLITSIITGFMV
                     RMPINEISTTISTGFGNTLGSIGIVIGLGIIFGNILSESRATESIAKGLLAKTGEKNS
                     ALAVTTAGFLISIPVFMDAAFVIMMPIIKYVSRVTKKSLMVFVCALGVGTIVGHALVI
                     PTPGPLAVAANVNANVGSFILYSIIVAFPAALIGGWIYGKRFEKYPAYAIDENDREKN
                     LEQGKDSIKIEDDDSSKVPGFGISMFSLLFPILLILISNVFSMFLEKGSTMSGVLAFI
                     GDKNIAILLGILVAIGFLKKYINKPMGDVVIEAADSAGLILLITGSGGAFGSVINASG
                     IGNFLVDTMSGLSISVVVLGFLLSALLRISQGSATVALVTTSSILGPTILATGMSPVL
                     VGLAICAGGVGFSLPNDSGFWVLSRFSGLSVKDTLNSWTIGGTIAGVVAFIMVLLLSV
                     INGIIPLPGL"
     gene            17856..19292
                     /locus_tag="KPD58_001243"
     CDS             17856..19292
                     /locus_tag="KPD58_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HBG0265025.1"
                     /translation="MREVKPISIDILNTFKQVDEDRLNKLLADELKHLDRKIVVLDDD
                     PTGVQTVHDISVYTDWDKDSMEQGFNEKNSMFFILTNSRGFTVAQTTKAHKEISKNIV
                     DVSKKVNKDFIIISRSDSTMRGHYPLETNLLKSEVERLSGKLFQGEIIMPFFKEGGRF
                     TIDNVHYVKEGEILVPAGMTEFAKDKSFGYKSSDIGEWCEEKTKGKYKSSDMIYISSK
                     ELRELNIEAITEKLKKAEGFNKIIVNATDYVDVKVFTIAFIRAVNSGKEFMFRSAAAI
                     TKVLGGVSDKELLTKDELVSKGNTNGGIILVGSHVNKTTQQLEELKNCKYPIEFIEFN
                     QHLVLQENGLKNEVKRVIKVVEEKISRGKTVAVYTRRERFDLDTNDKDKQLMISVEIS
                     DAVTSIIGMLNVRPNFIIAKGGITSSDVGTKALRVKKATVMGQIKPGIPVWMTGKESK
                     FPNMPYIIFPGNVGEISTLRESVEILMG"
     gene            complement(19478..20332)
                     /locus_tag="KPD58_001244"
     CDS             complement(19478..20332)
                     /locus_tag="KPD58_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265026.1"
                     /translation="MSTFYIQFLYFVIYSFIGWCCETTYCSVLQKQFVNRGFLNGPFC
                     PIYGFGALAIVATLTPFVHNIPLLFLFSIIITSIMEYCTSFILEKIFNMTWWDYSKHK
                     FNIHGRVCLENSLMFGVLSLIVMLIVHPIVVDFINSISKNVLFIFAISIEIYFVLDLV
                     ITVHTILQLNGKLKQINLIIKELKDKKEYYKLITQETIENKLESLVENRLDTLEDRFE
                     ILKEKENYRYAKNRIDELKNKLNTLLSNHKLLHRRIIKAFPNISSNKHTDILNKIKEN
                     IKSIKKDK"
     gene            complement(20600..20923)
                     /locus_tag="KPD58_001245"
     CDS             complement(20600..20923)
                     /locus_tag="KPD58_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HBG0265027.1"
                     /translation="MERINCNVINCSHNNSHICYADKIFINGEKAHNDRHTCCSSFLD
                     ESIYSNLSSNVNNRNCEELVCTVKNCKYNKDGSLCTLDNILVAPETDRVNLYIETCCS
                     SFECK"
     gene            21126..21755
                     /locus_tag="KPD58_001246"
     CDS             21126..21755
                     /locus_tag="KPD58_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265028.1"
                     /translation="MFEKKKKELFPWKISALILVGVFVLSSGVYIGTRLKHNGVQTGE
                     VVSTSANMNEKSKEAFGLNKNCEVWLQSEGENETNYKRTLTMAGIVPESLLDKTKSEI
                     VSYFKKEYPTKKIKSMDKNEIILVESKYSDNKDVQAMDVTIKGKYTIENDNGNIALYK
                     YDDSGSKTLVEKTKIRVDSLPKTVQDEIKKGVVMDTEEEAYSRLEDFAS"
BASE COUNT         8194 a         2546 c         3579 g         7499 t
ORIGIN      
        1 tataattata gaagggaaat ttaagatgga ttttaaaaat ttacagtatg aaagtttgtc
       61 atatattatt tgtaacttgc aaaaaaactt taaactttat tgtgaaaaat gtttaaaacc
      121 atataaactt acgaatggtc tatatttcta tttaatttat attaataaaa ataggaattg
      181 ttctttgaat gatgtttcta cagaatttga agttgataaa gctcatacaa caagaactat
      241 atctaggtta gaacaagatg gatacataga aaaaatccaa aaccctaatg atagtagagc
      301 ttttcagttg agagtaacag ataagggtga ggaagtatta ggggatataa aaaacatatt
      361 ttcaaagtgg gataatcaca taaaaaaaga gttctcggat acagaatata aagaactggt
      421 aaagaattta catgtggtaa aggatataaa aacagctgtg gaggaagaat aggatgtatt
      481 gtaatttatt aaaaccaatt aaaattggaa atttggaatt aaaaaataga gtatcttttg
      541 cacctacaag catgggtctt aaattggaag aaaaaattaa aaagttttct gatattgcta
      601 agtcaggagt agctctgatt actctaggag atgtatctat tagacctagt tttcataagg
      661 tagctataag cctatcggat gaagatggag tcattaaata caagaaaatt gtagatgaaa
      721 tacacaattc tggtgcaaag gtatcagcac aactattttg ttcagattat gatgttaatt
      781 taattaaaga tactatgaat atgggaatta cttcacatga tgagataaaa aaaataatga
      841 atgacggagt aaaagattac ataacaaata tgcccaaaga agaaattaaa aatattataa
      901 atttatttaa agtaacagca ttgaatgcaa aaaaagcagg ttttgacatg attcaaattc
      961 atggtgatag gcttgtagga agtttttcat caagtatatt taataataga aatgatgaat
     1021 atggtggaac gtgtgataat agaagtagat ttgcaagcga aataattagt agtatccgag
     1081 atgaagttaa ggacattcca atagactata aatttgcaat aagacaagaa aatcctcatt
     1141 atggaaatgc aggagtttta ctttcggaag ttgagtattt tgttaaaaaa tttgaatccc
     1201 taggtgtaaa cagttttcat gtaacacttg caaatcattc aaaattagaa gatactatac
     1261 caaccaataa tcatccttac tttaaagatg aaggctgttt tttatattta gctgatgagg
     1321 taaagaaaca cacaaatctt ccagtatgtg gggttggaaa attgtctagc ccagatttta
     1381 tagaaagtat tatttcaaat aatagagtag atatggtttc tatgtcaaga caattactag
     1441 cagatagtaa ttggttacag aaagtaaaag atggaagagt agatgagatt aaaaagtgct
     1501 gttattgtaa taagaagtgt gcagatgctt tacaaacacg ctctcagttt ggatgtatac
     1561 tagattagaa tatccttata aaaataaata atttgttaag gttaatatat ttttggtgat
     1621 ttagataaat atttagataa agaggaggag acatcatgaa aagtttagtt ttatattcaa
     1681 gtttaactgg aaatactaaa aagattgctt atactatata tgatgaaatt caagaagaaa
     1741 aagacattaa agatgtaaat gaattagttg attatggtat tgactatgaa aattatgata
     1801 tagtattctt aggctattgg gtggacaagg gaatttgtga taaaaattca aaacaagtgc
     1861 ttgaaaatat tcacaataaa aagatagcac tatttggaac aatgggagca agtaaaaaag
     1921 gaagttatgg agctagtata atagaaaaga tagaatcaat tatcccaaaa gataatgaaa
     1981 tactaggttc attcatatgt caaggtaaga tagcagaagg attgaaagca aaatataaag
     2041 agatgttaaa actttctcct gacaacgaac atattcgcca acaattaaat aaccatgagg
     2101 aatctcaaag tcatccagat gaacaagaaa tatatgaagc cagtatgttt gcaaaaaata
     2161 tgatgattaa agctagtatt gtgtagtagt taaacaataa gttcatataa gatatagaat
     2221 aaataattta gtaaaaaagt atagcaaata atttgctatg ctttttttat gaaatcaaaa
     2281 aaataataaa aatacttgac tgtaaacctt gtttatacat tattttatag aaaataagat
     2341 agttttaaat ataaaaaaag atatattatt gaaaggagat attattgtat gaaaacttat
     2401 aaaagataat tagtttgatt ttaagatata ctaaatatac aattttatag agtgaaaata
     2461 tttaagacta tattatagtt ataaattaat agaatagaga ggtagtgttt aaattgcaat
     2521 aacagtattt ccaaagttgc cacctcaaat tcctatccaa tggaataatg gtgtagctaa
     2581 ttatgtagat aaaaaattta tttatgccta ccctgtcatt tgtgttgtat tcagaatcct
     2641 tttacgtcca tttatatgga gatggttaca aatacatgct tttatagtga tactattgca
     2701 gattatttaa cgaattttat gtgttttgta gcgttgtcta tagaaatatt ctctatattt
     2761 tttatttaca atattgccag acatgttaca ataattattt ttatagatat acttgtattt
     2821 attgctttac tgataatagg tgtgtataaa ttatttaaaa aatacaatta atttttaaaa
     2881 ataagtaata agtgatagtg taaaaagtag ccactgcaaa ttatggattg tagtggctat
     2941 tttattattt gtatatgaaa ttattttgtt tttctaataa gttacgatat attatggttt
     3001 atttcactga taaaaggttt aggtatttta aagtaaaaag ttgtatattc taataaaatt
     3061 taaacatttt gattctttaa aattagagaa aatacattgc ttttattaaa aaattaaata
     3121 ttaagaattt aatttctgca tcttatttag aaaagataag aaatcaactt tatatatttt
     3181 ttttggcctt cctctttgat tatcgagttt tgtatttaca atagttgcaa ttcctgcttt
     3241 atataagata ctcaatattc tattagcact tctaagagtt atgttcatat agtctgctaa
     3301 tatttctgct gacacattat ttgaatttct atcattaatc atacacatga ttttagatac
     3361 atttgtatta gatagaggta taaaagaagc aactttattg gctattgagc tatcttcaat
     3421 attattagaa tttttatttg aatataaagg acctattgta tcattagtat catttactat
     3481 aaaagtacag tttccattca aagaagcaga tttaccatta gcttgacgag cattttgctc
     3541 tgcatgaata tttgtatttc caatgcccca acctatattt aagctatatt taatttcttt
     3601 taaatcttct gaaataaaaa aatctgtata gttattggtc atattgttta acatttcttt
     3661 agtagtgtgt atctctattt tcatatcatt ttttttgacc tgaactccat agaatccatg
     3721 tcttgataga gaatttaata tagtgttata tatttgattt tgtatctcat catttttaaa
     3781 tatcaaatct tcatttatgt actctgaact atttactgta actatacaag ttgcagattt
     3841 atttttgtct aagttttgta atgttatttc attcaccaaa gatttaaatg tattcaggat
     3901 tgattctgta gatggaacca tgtaaataca atcatatcca agcttattaa gttcacaact
     3961 aatgatgtaa tttctagtaa aagaaaggtt aatcttatta tctcgcaata agtctaaatg
     4021 tatttccaat aatttatcat ttaatttttt aaagtcatat acaggttgag ggatattaaa
     4081 atctttaatt gtatatggac atttattaga gtctattaat gaatatatat cataataatt
     4141 gttgtcctct cttaaaaaat ccataaatga ttttgtaaag tcaatatctt tttggctatt
     4201 taataatttg aataagtatt tataaaaatc tctttcatca agctttaaaa aatttaatgg
     4261 aacttttatg tcttttatat ttattataag ttcactatat ggaactatac cactgcatac
     4321 aaatccatca aaattatcta cttgctcata gtatatatta ggtaaattat taaaaggatt
     4381 ctccccttta agctcaataa atgtaatttc acaagtatca tttaattctt taagagcttg
     4441 tttaaactta tctgacataa aataagatgt tgtcaaaaaa ccaattttat atttcataaa
     4501 acctccttta aaatttcgag agtattgtaa ctaatcaatc aatttataac ctcattttat
     4561 aatagtagat aaaacgcgtc aatttatttt acaaaaggaa aattgaaaga aaaatcagaa
     4621 aatataaaaa aagactgaaa aatatgttgg ctgtcaagaa taattgtgct attttgtaat
     4681 taaggactag tcttttaaga tttcctaaaa taaacttaga ttttaggaaa gaaatacatt
     4741 ttatttgaaa ataaaggaga agattatgaa tacatattta aatgaaattt ctaagtttgt
     4801 tgatgagaat agagaagaaa tagtttcatt atggaaagag attgttaata tggaaagcta
     4861 tacacattgt aaagaaagcg taaataaatt ggctaagaga ttaaagtttg agtttgaaaa
     4921 agaaggttta gattgcgagt tagtagatgt tggagataat ggtagtacat tgataggtac
     4981 acttggttcc aatatagata aaaagccaat aattttttca ggtcacatgg atactgtctt
     5041 tgaaacaggt acatttggag agaatccatt taaaataata gaaggaaaag catatggccc
     5101 aggtgtttta gatatgaagg gtggtataat aatttcctta tatgttatta aagctttaaa
     5161 taaaatagga tacaaagaaa gaccaataaa aatagtattt tctggagatg aagagatagg
     5221 acataaggat tcaacaggtg cagatgttat attgagggaa gcaaaaggtg gtctttgtgc
     5281 ttttaatatg gaaactggat tagtagataa tagtttgtgt gtaggtagaa aaggaagaat
     5341 aggatgtaat atacatgtaa aaggtgtaga aacacatgca ggtaatgatt ttgaaggtgg
     5401 aagaaatgct atagaagaaa tggctaataa gatactacgc atacaaaaat tgactaattt
     5461 agatgttgga actacagtaa gtgtaagtgt gattaagggt ggaagagtgg aaaattctat
     5521 tccagaagat tgtagtataa aagtagacct tagatttgaa aaagtagaag aaatggagaa
     5581 tgttaaagaa caagttagag agatatgtaa agaaacttac atagaaggaa cttctacaga
     5641 agttaatttt ataagtgaaa tgatgccatt tgaaactaca gaagatgtta tgagatttca
     5701 tacatttgta aatgaagtgt caaaggaaaa tggatttgga gaattaaatg ctaagagact
     5761 tggaggtagt tcagatgcaa gttatttaac tattgcaaac gttcctacaa tctgttcatt
     5821 tggtgtaaga ggggaatgga accacacatc aagagaatat gcagtagtaa attctatgtt
     5881 tgagagggtg aaattaattt ctacagttat tttaaactta gataaatttg aggtataaag
     5941 ggggatttta tatggatgta acagctggag ataataaggt aacatggaaa gggtatttat
     6001 ctttatttgt attgatagta ttattttcag gagtgtttaa agatagtcaa ggcttcctta
     6061 aagcattaga ttttgctaat ctgacaggtt catttggtac tattgcagat ggtgtagact
     6121 ttatgggtaa aggaggaacg ggagcaaaag atggtttctt atttgctctg actttgattc
     6181 caactgtctg ttttgctgta ggtcttatag atgtagttga atctatggga ggaatgaaag
     6241 cggcatcaaa gatatttaat ccattgctta gacctctgct tggtatacct ggaatagcag
     6301 gaatagcatt tgtatcttca tttactagtt cagatgtagc ctcaataatg acgaaagaat
     6361 tatttgaaag tggggaaata acagatgatg aaagaactat atttgtagca tatcaatatg
     6421 ctggttcagc tgttatactg aacacaataa atactcaagc tccactttta ccaatagcat
     6481 tattggcatt aggtccaata atccttatag agatattctg taaaatactt ggtgctaatc
     6541 tagttagatt aatcataggt gtaagaaata aaaagaataa ggctaaggag gctgtgtagt
     6601 atgagtcaac aaaatactgg aaatgcaatt gtaaatagta aacctaagaa aactgctgtt
     6661 gagatattta tggggggagc aaaaaaaggt ttttacatag gtgttgaaca gatattacca
     6721 gccatgataa tgggttatgt aatagtgcag tttttacaat taactggttt agttgatatt
     6781 ttaggaaata tttttggacc agtaatgggt atttttggac tacctgggga atctgttgta
     6841 gttttgatag cagcattctt tgctaaggca gcaggagcag ctacagctgc aaacttattt
     6901 gcacaaggtc taatcactgc accacaagca accatattaa ttatgccatg tatgttaatg
     6961 ggtactctag ttggtcatta tgccagaata gtgcttgtag caaatgccag tactaagtat
     7021 agaatgttac tgttggcagt tcctatattt gatgcaatag tggggatgtt aatagtaaga
     7081 gttttactca ctgttatggg attaatgtaa ataacataaa attataacct aactataaat
     7141 gatatataag aattaatata tacaaggatt aatgagtagt aattgatata tagtaataaa
     7201 ctaagatagt aattaatgtg tatattaatt aaaaaaagag agtatctcaa agtgaacttt
     7261 ttagttcata agatactctt tttgtatgaa atcaagtata ttttcataat ttcaacaatg
     7321 aaagagaggc ttatctctat tttgagacag cctcttcttt taaatttata tttctagaat
     7381 ttatgttttt tatttatcca acattaacat tagttattag aaataatttt aagaaaaata
     7441 tactttcatg ttaatatcag aataaatgtg tttttaaagg atgatttgtt ttgaattttc
     7501 acgaatatct acttctatga ctttttctat tgtatctcct ctaaaaccta ataaattact
     7561 tgtcaagttt gcagtggaac aagaatgatt aggtataatt cttattctat caccaatttt
     7621 tatatcagtt tcaccattta ctttaatttt tccaacttct tctgaaagtg aatcaatagt
     7681 aagttctgga tgtccacata ccctaccata accaacaata ctactatttc catgtgcacc
     7741 tttatcaagt ccaaggcact tacttccaca atcaattaaa tagtatccac tgctattttt
     7801 tgagattaca gtgcacacaa cagtaagagc acacatatct tcagtagcac aacctaaacc
     7861 tatctgtata gcatcatagt atacatagtt tccaggtctt acagcagtta tagttttaga
     7921 agcagcttca gattcaactg ttggagtact accagaagcg acaatttcta tattaaatcc
     7981 attatcagtt agagctttgt atgctttctc catagaagaa tcttcttgaa cacaaacatc
     8041 tggaacacct tctggagaac tacaaccata cacttgacct ggatgcgaac ttataccaac
     8101 aatcttgatg ttttctagct tagaaatttc agtagcaagt tcaaccactt tttctggttc
     8161 aaccccaaaa cggtgtaagc cacaatctac aataataagt agcttgcact ctatttttga
     8221 tttagaaaaa gcttcactat aagccttagc acattctata ttatctgtag cacagattaa
     8281 atttattttt gatgccatag tcacaattct atttatattt acttctccca tgtaaggata
     8341 agccaacata atatcttcaa aacctgcttt tatcagagct tcaccttcat caagtgttcc
     8401 aactagaaaa ccactagcac ctgcattttt ttgaagtctc gctatgtaag tacttttatg
     8461 tgtcttaagc ataggccaaa gttgtttatt attatttgta caaatttgtt gaattttatc
     8521 tatattcttt tctaactggt ctaaatctag caaaaaattt ggagtagcga cttctgaaat
     8581 tttcatgtta tttaccacct ttcaacaaaa gtaaattaac tataagtatt ataattatta
     8641 tattagtaat atcggcttta taatattaat agcttataat tatattatag aactagcgtt
     8701 aacagagagc aatatttatt tgtaataatt agaattttca ataagttagt gtagttttaa
     8761 attatttata taatatattt ttaattttat gcaaagttta tttaaattag tttgaggatt
     8821 tattaatagg gtattattga atgagtaaaa aagtgatgtt tacatagaat aatcacacta
     8881 taaattaatt tagaacctaa aaaattaatt gagcaaattg gaaaagagaa ggataaaaaa
     8941 gatatttgga tagttggtgg aggcgatata gtaaaattat ttatgaaaga taacttaatt
     9001 gatgattatt atatatatat attgccaata atattgggga gagggatttc actatttaag
     9061 tctggtatag acaagactaa tttgaatttt aagaaagcta gtaatattgg agaacttgta
     9121 aaattggaat acagtaaaaa acaaaaataa attacatgcc aatttgttac aatgttatga
     9181 aacgaagcat ttaaaagact tttggctata tgaattgaca ttacttgtca aatatgttaa
     9241 catataagcg acttgtcgga gggtgtatta aaaagaatat gctggagaag agagtgcgtt
     9301 aactcttttc tccttttttt ataaaattaa gcatttataa atttagtatt attacatgat
     9361 tacactatct caaaaattaa gaaaggagaa aaatatgaaa ttaattttaa gttatttaaa
     9421 gaattataag ttattaatag tattaaacat actggctata tttagttttg cattagtcga
     9481 gttggggatt ccaactataa tagctaaaat aattgacaat ggtattgcaa atcagaatat
     9541 agcttacata aaacaaatgg gaatagtgat tgttattatt tctattatag gggttgtagg
     9601 tagtatattg ttgggttatt gttctgctaa ggtatcaaca agtgtaacca gagatataag
     9661 aaatgacata tttgagaaat cacaagaatt ttcacatact gaatacaata aatttggaat
     9721 ttcttcaatg attacaagaa caacgaatga tgcatttcaa attcaacaat ttgttaatat
     9781 actactaagg actgccttat taactccagt tatgttcatt ataagtataa taatgacaat
     9841 tagaaccagt gtggaacttt ctatagtatt ggctatatca gtacctttta taattattgg
     9901 agtagcaata atagcaaaag tatctcaggc aatatcttca aaacaacaaa aaggactaga
     9961 taaattaaac cttatatcga gagaaaattt aactggaata agagtaatta gagcttttgg
    10021 aaatgatgat tatgaaacag agcgatttga aaaaacaaat acatattatg ctaatgtttc
    10081 taagaaacta tttaagctta tgtcaattac tcaaccagca ttctttttac tgttaaatat
    10141 agctgttttg gctgtatttt ggatttcaag tgagaagata aatattggag agcttcaagt
    10201 tggacagttg gttgcatttt tggaatatct tttccatgcc atgttttcaa ttatgctttt
    10261 ttcaatggta tttattatgt atccaagagc agaggtatct gcaaatagaa taaaagaact
    10321 attaaatgaa gaaccattga taaaaaatcc tgaaaatgga attaaggata ctgaaaataa
    10381 gggtatcata gaatttgata atgtaacttt tacctatcca gatggagaag cttctgtact
    10441 taaggatatt tcatttactg caaaaacagg tgaaacagtc gcttttatag gaagtactgg
    10501 tagtgggaaa agtactctaa ttaacttgat acctagattt tatgatgtaa cagaaggtag
    10561 cattaagata aatggagtag atataagaga atatgattta aaagctttaa gaaaaaagat
    10621 aggattcata cctcaaaaat cactgctttt tactggtagc atagcaaata atattagatt
    10681 tgggaaacat aaggcaggag agtcagaatt agaatattcc gcaaaagttg cacaagcata
    10741 tgagtttata tcaaaaaaac ctagaaagtt tgatgaattg attagtgaag gtggagcaaa
    10801 tgtatcagga ggtcaaaaac aaagactttc tattgcaagg gctattataa gaagacctga
    10861 aatatacatt tttgatgaca gtttttctgc acttgacttt aaaactgatg caatacttcg
    10921 tgctaaattg aagaaggaaa caaaagatgc aattgtattg attgttgccc agagaattag
    10981 ttctattatt gatgctgata agattatagt actcaatgaa ggacaagttg taggaatggg
    11041 aactcataaa gaattattaa aaaactgtga aatatactat gaaattgcaa catctcagtt
    11101 aaaaaaggag gaattagaat aatgaaaaag tctaaaggtt ttaagaaaac aattggtaga
    11161 cttcttccat ttgtaaagaa atataaattt tcctttatta tagctattat atgtataatt
    11221 tcagcagcta ctatgaatgc acttgcacct aaaactgaag gattgataat aacacaattg
    11281 actaaagatg taattagtat cgcaaaagga gtaccagggg cttccgtaaa ttttgattat
    11341 gtaacaaaaa tattggtgat tttagcatgt atatattttg ccaatgcaat ttttacgtat
    11401 acttcaagtt ttctacttac gaatgctatt caaaacacaa tgagagattt aagaaatgaa
    11461 gtcgaaaata aaataagaag attgcctatt agttattttg atagtaatag ttttggagat
    11521 gtattaagtc gtatatcaaa tgatgtagat acaatatcaa atgcattgca acagagtttt
    11581 atgcaaattg taaactcaat cttagtcata attttggctc tttcaatgat gtttacaatt
    11641 aatatatata tggctcttat agctcttttt ataattccaa ttagttattt tgtatctaaa
    11701 tttgtagtaa aaaaatctca aagtagattt tcattgcaac aaaatgctct tggaaaatta
    11761 aatggaaaag tacaagaaat gtatactgga tttaatgaaa taaaactata tggtaaagaa
    11821 gaagattcta taaaagaatt taaaaaagta aatcaagaac tttgtgaaaa tggatttaag
    11881 gcacaattta tatcaagtat gatgaatcct atggtttcat tagtaactta ctttggaatt
    11941 gcagctgtgg cagttgtcgg ttctatatat gcagtaagtg gaggaataac agtaggaaat
    12001 ttacaagcat ttgtgcgtta tatatggcag ataaatcagc cactttcaca aatgactcag
    12061 ttatctacag taatacaatc ttcttttgca gctatagaac gtgtatttga aattttagac
    12121 gaagaagagg aaatacctga tgttgaaaat ccagttaaga tagaaaatgt aaaaggaaat
    12181 gttacatttg agcatgttaa ttttggatat ggagaaaatt caactttaat agaagattta
    12241 aatgcagaag taaaaagtgg tcagatggta gctattgttg gacctactgg agcaggtaaa
    12301 acaacattaa taaacttatt gatgaggttt tatgatgtaa agaaaggtgc tattaaaatt
    12361 gatggagtag atataagaga tatgaaacgt aaagatttgc gttcaatgtt tggtatggtg
    12421 cttcaagata catggttatt taatggaacc atttttgaaa atatagaata tggtagattt
    12481 ggggctacta aagaagaaat tatacaagca gcaaaagtag caaatgttca tcactttata
    12541 accactcttc cagatggata caatatgttt ttaaatgaag aagcatctaa tatatctcta
    12601 ggggaaaaac agcttttaac catagctaga gcttttatat cagacccttc aatattgata
    12661 cttgatgaag ctacgagttc tgttgatact aggctggaac ttatgcttca aaaagcaatg
    12721 agaaatctta tgaatggtag aactagtttt gttatagcac atagactttc tacaattcgc
    12781 aatgctgact tgattttagt tatgaataat ggaagtatta tagagcaggg gaatcatgat
    12841 gaattaatgg aaaagggtgg attttatgag aagctttata atagccaatt tgcagacaaa
    12901 gaatcagaat agataacata aacttttgta taaaattttg tatcaaattc aaatattaat
    12961 catataatta aaaagagtat cagtaagttg tttttttata gatttactga tactctttga
    13021 ttttaagact aaaggtattt atgattggta cttataattc acaactagtc ttgttacatc
    13081 tgataaccag aattacagta cttttctttt ttaccttttt gttctctaac atataacaat
    13141 tcgcttaact tatcaatgta ataaccatca tcacttatgc gaattaaact agtcagataa
    13201 tatccatctt tgcttctagg aatcttttta tttattgcta aatcaatagc tgtctttgct
    13261 gcttcaaaat gctttaaatg tgtatgacct tcttcaaaac tctttctagt attataaact
    13321 acataacctt ttttaacttg aaaaactata aattctttct tttcatatat tttattaaac
    13381 attcaatcag cccttctcaa gatagtttta tagcataaca ttagaagctt ctaaaaaatt
    13441 ttacaaagaa cataaatatt ttataaatta taaatctaaa actataattt ataaagataa
    13501 ctttctgatt atttatatga taattcatat agattaaaag taaattatct ttaggttaaa
    13561 caaaagtaaa ataaaagact agttataata tttatatgaa aaagtacaaa atatagtata
    13621 tgaatataga aaatttaaat tatttattta cagtttttat ttccataagt atatttacct
    13681 tcagatacct tgtcatcatg gaaatatatt ctttcacaac ttttatctcc ccatagaatg
    13741 ttttttgtta ttttatggga agtatctcct gtaaatactt cagctacagt agtatctgta
    13801 acatcacagt aaagagaagc aaatttttta aaccaaggat tttcctcaaa tcttaaaagc
    13861 caggaagctc cataggggca atgtaactct tcaaattcag gaacccatcc tagaaatgga
    13921 acatcagttg tgctaataat attgtctgcg attaattcat ctccattttc atgagctctc
    13981 tctctcatag caatagctct ttcttcagac ctttcaaata aaacttttct taatatttcg
    14041 ctagttttat cttcaccaaa ctcttcaaat aacactttgg aaaaataaaa atataaatca
    14101 ccaaaatgtt tgcatgccat acgaacttct tcaatagcat aatctttgtc aatagtttca
    14161 ttagttacaa catcttcgtt caaaaaaatc actccttaga ataagtatta gttacaatat
    14221 aattatagaa tattttttat aaattaggaa attaatttga gatagatata tttttaataa
    14281 atgaaggtat aatgctaaaa taacttattg ttacaaaaat cttctaaatt aatgtattta
    14341 ggagtttcca tatgaatgag agtagttctg taaatatctt caatacatcc atttgcaata
    14401 ttattaaaaa tatctgtgtg ctcttttaat gttgtttcaa gccttccttc atgtgacaat
    14461 gaatcgagag ctaatttttt tattctgtac atataattgt taaacatttt gtcaaattca
    14521 gtattttgag catatgaaac tatagttgtg tgaaataatg tatcatattc tgcaaatgag
    14581 tttaaatttt tatcacctaa taaaatatct ttttgtttat ctaaaagatg ttttaattta
    14641 cttatagttt ctactgctct agcttctttg tattgacttg taattaaaaa agtacaaaat
    14701 ccttctattg cagagcgtat ttggaatatc tctacaattt catttgctgt aatttgatgg
    14761 attctaaatc ctttgcttgg gattatatct ataaatccat cttgactaag tctttgaaga
    14821 gcatctctta taggtgttct agaaatacct atttcactag caagttttgt ttcagaatat
    14881 atttcatttt ctatcatttt tccagatagt atcaattctt ttaagtagtc atatgcttgt
    14941 tcttgtaatc ttttaaatgt agccatactt aataataccc ccctaaacaa tataaaaata
    15001 caaaaactta tataatttta agcatacaac atagtgaaaa taaggtcaat cttttgcaat
    15061 ttaaattgca gataagatta aaaaatataa aaaattattg aagtgtgaag catgattaac
    15121 attaaaggca gattaattaa aactaattga aattagttaa aatacaaaac tgtaaaagta
    15181 gtatattata tttgaaaaca atagtatgta ttaaaactta tataggatga ctaaatataa
    15241 taaatgactt taaaaattac tatttattgt taaaataaat acttttatat tttatattga
    15301 ccggtatact ggtatgtagt atactctaaa acataaaaaa cgttttcaaa aatgtgaaga
    15361 tatggagggt tataaaatga aattgggatt tattggatta ggaataatgg gaaagccaat
    15421 ggcaaaaaat ttattaaaag atggatgtaa tcttttggtg tatgacatta ataaatctgc
    15481 tgtggatgaa cttatatcat gtggagcaaa atacgcaagt gttttagaaa tgggtcaaga
    15541 atgtgatatt gtatttacaa tccttccaaa tggaactatt gtgcaggata tattgtttgg
    15601 gattgatgga ttggcaaaaa ctttaaaaga aggaagtata gtagtcgata tgagttcagt
    15661 tactcctaca gaatcaatat tgtgtgcaaa taaattaaaa gatatgggct tagaatttat
    15721 agactcacca gtaagtggag gggagccaaa agcgattgat ggtacacttg cttttatggc
    15781 tggtggtaag gaagaaattt ataagaaagt agagccattt tttaatataa tgggaagctc
    15841 atctatttta attggagata atggtagtgg aagtgttaca aaacttacaa accaagtaat
    15901 agttaatttg acgatagcag cagtttctga ggcatttgta cttgcagcaa aagcaggtgc
    15961 agacccagaa aaagtatata aggcaataag aggaggccta gctggtagta caatattgga
    16021 tgcgaagata ccaatgatta tgaatcgtga ttttaaacca ggtggaaaaa tatctattaa
    16081 tttgaaagat attaagaatg taatgcaaac agcacacaat ttagatgtac cgctacctat
    16141 gacaagtcaa ttattagaaa ttatgcagac attaaaagta catggacatt tggaagatga
    16201 ccatagtgga atagcacagt attttgaaaa gttggcaggt gttgaaataa aaaaacacga
    16261 taattaatat ttaaatatat caatataatt aaatacatat tattaggatt agattatata
    16321 aatacagcga ggttaagtta tcatagtaaa agaaaaataa aattgataaa taataaagta
    16381 gaatgataaa ggggtaaaga ttatgattac aggatatgga cttttgatag catttgttat
    16441 atctatagga attttacttg tatcgattat taaatttaaa gtaaatccgt ttttagcatt
    16501 actgataaca tctataatta caggctttat ggttagaatg cctataaatg aaatatcaac
    16561 cacaatatcg actggatttg gaaatacatt aggtagtata ggtattgtaa ttggacttgg
    16621 aataatattt ggaaatattc tttcagaatc aagagcaaca gaatctattg caaaggggtt
    16681 gttagctaag actggagaaa aaaacagtgc tttagcagta actacagcag gttttttaat
    16741 ttcaattcca gtttttatgg atgcagcttt tgttataatg atgcctataa ttaagtatgt
    16801 ttcgagagta actaagaaat ctctaatggt ttttgtatgt gcattaggag ttgggactat
    16861 agtaggtcat gcacttgtaa tacctacacc aggaccttta gcagttgcag caaatgtaaa
    16921 tgcaaatgta ggtagtttta tactttattc tataattgtt gcttttccag cagctcttat
    16981 aggtggatgg atttatggaa aacgttttga aaaatatcca gcttatgcta ttgatgagaa
    17041 tgatagagaa aaaaatttag agcagggaaa agatagcata aaaattgaag atgatgattc
    17101 ttctaaagta ccaggttttg gaatttctat gttttcttta ttgttcccaa tacttcttat
    17161 acttatatca aatgtattta gtatgttctt agaaaaaggt tctacaatga gtggagtact
    17221 tgcatttata ggagataaaa acatagcaat acttcttgga atattggtgg caataggatt
    17281 tttaaagaaa tacataaata aacctatggg agatgtagtt attgaagctg ctgattcagc
    17341 agggctaata ttattgataa caggttcagg tggtgcgttt ggcagtgtta taaatgcaag
    17401 tggtataggt aacttcttag ttgatactat gtctggtctt agtatatcag ttgtagtgct
    17461 tggattctta ttaagtgcct tgcttagaat ttctcaaggt tctgctactg tagccttagt
    17521 aacaacatca tcaattttag gacctacaat tttggcaact ggaatgtcac cagttttagt
    17581 agggcttgct atatgtgctg gaggagttgg tttctccctt ccaaatgatt ctggattctg
    17641 ggtactttca agatttagtg gattatcagt taaagatact ttaaactctt ggacaattgg
    17701 aggaaccatt gctggagttg tagcatttat aatggtttta ttgcttagtg ttataaatgg
    17761 aattatacca cttccaggtc tataaataac aaactattgt ttatatcata taaattacat
    17821 ttagactata caaattacat aaaggggcgt tttaaatgag agaagtaaaa ccaattagca
    17881 tagatatact taatactttt aaacaagtag atgaggacag attaaataaa cttcttgcag
    17941 atgagcttaa gcatttagat agaaaaattg ttgtattaga tgatgaccca acaggtgtac
    18001 aaacagttca tgatatttct gtttatactg attgggataa ggatagtatg gaacaaggtt
    18061 ttaatgagaa aaatagtatg ttttttatac ttaccaattc aaggggattt actgttgctc
    18121 aaactacaaa agcacataaa gaaatttcaa agaatattgt ggatgtatca aagaaagtta
    18181 ataaggattt tataatcata agtcgtagtg attctactat gagaggacat tatcctcttg
    18241 aaactaattt attaaaaagc gaagtagaga gattatcggg gaagttattt cagggtgaaa
    18301 taataatgcc attttttaaa gaaggtggta ggtttactat agataatgta cattatgtta
    18361 aagaaggaga aatacttgta ccagcaggaa tgactgaatt tgcaaaagat aaatcttttg
    18421 gatataaatc atctgatata ggtgagtggt gtgaagaaaa gacaaaagga aagtacaaat
    18481 ctagtgatat gatatatatt tcgtcaaaag aacttagaga gctaaatatt gaagctataa
    18541 cagaaaaact taaaaaagct gaaggattca ataaaattat tgtaaatgca accgactatg
    18601 tagatgtaaa ggtatttaca attgcgttta tacgagcagt aaatagtggt aaggaattta
    18661 tgtttagaag cgctgctgcc ataacaaaag tacttggcgg agtgagtgat aaggaactgc
    18721 ttactaagga tgaattagta tcaaaaggta ataccaatgg aggaattata cttgttggtt
    18781 cacatgtgaa taagactacc caacagttag aagaacttaa aaattgtaaa tatccaatag
    18841 aatttataga atttaatcaa catttagttc ttcaagaaaa tggtctaaaa aatgaggtaa
    18901 aaagagttat aaaagttgta gaggaaaaaa taagcagggg taaaactgtt gctgtttata
    18961 ctagaagaga gcgttttgac ttggatacaa atgataaaga taaacagctt atgatttctg
    19021 tagaaatatc agatgcagtt accagtataa ttggtatgct taatgtaagg ccaaatttta
    19081 ttatagctaa gggtggaatt acttctagtg atgttggtac taaagcacta agagtaaaaa
    19141 aagctacagt aatgggacag ataaaacctg gtataccagt atggatgaca ggcaaagaaa
    19201 gcaaatttcc taatatgcca tatataattt tccctggaaa tgtgggagag attagcacac
    19261 ttagagaatc agttgagata ttgatggggt agtatattag tgtattgaag tttaaacgct
    19321 taaatttact gtgttaaaag taaaataaac taaaattata aattaagatt aaaaataata
    19381 gtatattaat tagggtttag agtattttac aaaaaatatt ctaaacctta atcttatgta
    19441 ttagtatttt tattatttaa cttatgtgtt tattttatta tttatctttt ttaattgatt
    19501 taatattttc tttgatttta tttaaaatat ctgtatgctt attagagctt atgttgggga
    19561 atgctttgat tatacgtcta tgaagtaatt tgtggttaga cagcaaagta ttaagtttat
    19621 tttttagctc atcaatacga tttttagcat agcgatagtt ttctttttcc ttaagtattt
    19681 caaatctatc ttccaaagta tccagtctat tttcaactaa agattcaagt ttgttttcta
    19741 tagtttcttg tgtaataagt ttgtagtatt ctttcttatc ttttaattcc ttaattataa
    19801 gattgatttg ttttaactta ccatttaatt gtaaaattgt atggactgtg ataactaaat
    19861 ctaaaacaaa ataaatttct atagatatag caaatataaa tagcacattt ttggagatag
    19921 agttaataaa gtcaaccaca ataggatgta ctataagcat tacaatcaat gataatacac
    19981 caaacataag tgagttttcc agacaaactc ttccatgaat attaaattta tgtttagaat
    20041 aatcccacca agtcatatta aatatttttt ctaaaataaa gctcgtacaa tactccatta
    20101 tgctagttat aattatacta aacaaaaata acaaaggaat attgtgtaca aaaggagtca
    20161 aggtagcaac tattgctaat gctccaaatc catatatagg gcaaaatgga ccatttaaaa
    20221 aacctctatt tacaaattgt ttttgtaaaa ctgaacaata tgtagtttca caacaccatc
    20281 ctataaagct ataaattaca aaataaagaa attgaatata aaatgtactc ataagcacct
    20341 catttctagt tagtataaca ctatatactc gatattttat ttttttgatt aatttactaa
    20401 attcaattta catattgatt ataccaattt atagaaattt atcaaattta attacagtta
    20461 gaacattata aacatggtta taataatgaa attttattta aaataaataa gggtgtcaag
    20521 tataatcaaa ctaatagttg tagatactct acactatgtt agtttaatta catttgacac
    20581 ccttatattt atactatgac tatttacatt cgaaactaga gcaacatgtt tcaatataaa
    20641 ggtttactct atcagtttca ggagcaacca atatattatc aagtgtacat aagctaccat
    20701 ctttgttgta tttacaattt tttacagtac aaactaattc ctcacaattt ctattattaa
    20761 cgttgctaga taaattacta taaatagatt catctaaaaa tgaactacaa caagtatgtc
    20821 tgtcattgtg agctttttca ccatttataa aaattttatc tgcataacat atatggctgt
    20881 tgttatgtga acagttaatt acattacagt ttattctttc caaagttatt acctcctaaa
    20941 cgattattta ttataaatta tgtccatata attacaagaa tataaccatt aagagaaaac
    21001 taccttttag aaaatatcta ttcttagagt aatttgaaat ataaatatat tttcttctaa
    21061 aatttaccta aattgtataa aaacttacag cttggagaaa ataatagaaa aaggaggcgt
    21121 aatcaatgtt tgaaaagaag aaaaaagaat tatttccttg gaaaatatct gccttaatac
    21181 tggttggagt atttgtatta agtagcggag tttatatagg gacaagatta aaacacaatg
    21241 gagttcaaac tggtgaagtt gtaagtacta gtgcgaatat gaatgaaaag tcaaaagaag
    21301 cttttggatt aaataaaaat tgtgaagttt ggcttcaaag tgaaggcgaa aatgaaacta
    21361 actataagag aacacttaca atggcaggaa tagttccaga gtcattatta gataagacga
    21421 agtcagaaat agtatcatat tttaaaaaag aatatccaac taaaaaaata aagagtatgg
    21481 ataaaaatga aatcatattg gtagaatcta aatatagtga taataaagat gtacaagcaa
    21541 tggatgttac tataaaaggt aaatatacta ttgaaaatga taatggaaat atagcactat
    21601 ataaatatga tgatagcgga agtaaaacat tagtagaaaa aacaaaaata agagtagatt
    21661 cacttcctaa aacagttcaa gatgaaatca aaaaaggtgt agttatggat actgaagaag
    21721 aggcctattc aagattagaa gactttgcaa gttaatagta aattaatatt attttaagtt
    21781 aaatactaag taagttaaat actaagtaaa ttaataat
//
LOCUS       DAELLV010000039        21741 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.039, whole genome shotgun sequence.
ACCESSION   DAELLV010000039 DAELLV010000000
VERSION     DAELLV010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21741
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.039"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            134..1348
                     /locus_tag="KPD58_001247"
     CDS             134..1348
                     /locus_tag="KPD58_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBG0265029.1"
                     /translation="MNQEYVKGNPIFTNINKVPRQFPYLTDDIDTDVIIVGGGVTGCI
                     CAYYLAKNNIKSVILEKGRIAHGSTSVTTSLLQYELDDNLIDLTEVMTLKDALKAYNL
                     CTSALEELDTFIELYGNKCDYAKRDTLLYTANKLEVKAIKEEYNLRKENGFDVEYIDE
                     STNPFSFDLKSGLIAKNGGRELDPYKYSHHLIDVSLKNGLQVYENTEVKKVDLSNDKV
                     TAEVSYGYKVHGKKLIVATGYNTSLFTKRNFATKSNTFNIATKPLKNIASWKNNILIR
                     DNCDPYNYLRTTKDNRLIIGGEDVSFDDIENETLANEKYDILEQRLKSMFKDIKDIEV
                     EYKYCGCFASTLDNLGFIGPDNKNNNLWYCLGYGANGILFAILGGIMLSELYLGKQNK
                     NMKLFKVDRFDK"
     gene            1525..3438
                     /locus_tag="KPD58_001248"
     CDS             1525..3438
                     /locus_tag="KPD58_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0265030.1"
                     /translation="MKYGGIIMKEILVIAPTKGTYEKSIHIVKKNKYTNIDVVFGNLK
                     EGIPLAEKSINHGTRIIISRGGTYNMLKATYNIPIVEIKVDAYDIIKSYKEVKNSNEP
                     FGIIGFNNVIYGFDIIEEILNKKITMIEIEKEEEIYDAIEKYRKKGINTYIGDTTVAH
                     IVKRLNCKGILIESREENILRAIQQAEQILEATKDEQKRRLQIEAMTDFVHDGIITVD
                     KNFKITLFNKSAEKIFGIKKENALYHNVVDVIPNTVLPQVVETMEPQIGEIQNIGSTK
                     IITNRVPIIVDGQIQGAVSTFQDSKEISSFEHTIRRSLLSKGLSAKYTFDDIIHASEK
                     TKDCIELAKKYACYDTPMIITGESGVGKELFCQSIHNYSKRKNAPFVAVNCAAIPPSL
                     IESEFFGYEEGSFTGARKKGKAGVFELAHEGTIFLDEISEIPLELQGRLLRVLQEKQV
                     MRIGGDKVIPIDVKIICASNKDLKNMMRNGLFRQDLYFRINILTLYLPSLKERKEDII
                     KLSEFFIHKYSSKYNKTPLIITPNIKKALLEREYEGNIRELEGMMERAVIVESFDSIL
                     LEKLSNEYTNNIVDKDNSDFECSNLDLKTLEKKYIYKIYNENNRNTTKTCEILKITRS
                     TLWRKLKEIEDDV"
     gene            3709..4683
                     /locus_tag="KPD58_001249"
     CDS             3709..4683
                     /locus_tag="KPD58_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HBG0265031.1"
                     /translation="MHILKSVKKIPGGLMVCPLLFGALFNTVCPQALEIGGFTTSLFK
                     TGAMSILALFCLCNGAQINIRQAGKPLTKGIVLTATKFIIGAVLGIIVSKFIGPKGII
                     GITPLAIVATMTNSNGGLFAALAGEYGDSTDVGAISILSLNDGPFFTMLAFGVTGLAN
                     VPIVALFAALIPILLGFILGNLDEDIREFLAPGTTLLIPFFAFPLGAALNFNQIITAG
                     LPGVVLGLGVTIITGMGGYFVMKLIRAEHPAVGAATGTTAGNAAGTPEALAAIDPTLA
                     TIAAVSTVQVAAAIIVTAICCPMLVSYLDKREKKKKSIFKTNKNIEVN"
     gene            4704..4979
                     /locus_tag="KPD58_001250"
     CDS             4704..4979
                     /locus_tag="KPD58_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBG0265032.1"
                     /translation="MNAIIHANEKDNLVTCVRPLIKGEKVSIDGKSYIVNDNIPVFHK
                     MATEDVKKGDVVYKYGEIIGIATVDIKTGDYVHVHNIESTRGRGDKK"
     gene            4992..6155
                     /locus_tag="KPD58_001251"
     CDS             4992..6155
                     /locus_tag="KPD58_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBG0265033.1"
                     /translation="MKLMGYLRENGQFGIRNHVLIIPTSVCSSETATRIASLVPGAIA
                     IPHQHGCCQIGSDIELTAKTLIGFGKNPNVAAVLVVGLGCDGIQAKELASEIATTGKK
                     VDYVVIQECGGTLKTVSKGAEIVSKMAREVSKEVRVEFGMSEITLALECGGSDPTSGI
                     ASNPSIGVASNLLVDEGGSSILSETTEVIGAEHLLATRFEDEEMKDKFLKFVSDVEKR
                     AIAMGEDLRSGQPTPGNKAGGLSTIEEKSLGCMYKAGNKPFKGALEYADIVPPDKKGL
                     YFMDTPGQDIDSITGMVAGGAQIVIFSTGRGTPTGSPISPVIKITGNSDTYNKMPDNI
                     DINAGRIITDGAKIADIGQEIFNEIIEVCNGKHTKAESLGHREFGIYRISSTF"
     gene            6492..8771
                     /locus_tag="KPD58_001252"
     CDS             6492..8771
                     /locus_tag="KPD58_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG0265034.1"
                     /translation="MNFENTKNKKIIATLLLTLLLIVSLTCVTFIMNLNKVTHNETER
                     YLVEVSQSISTTINVKCKSIFRTLYSTGDTYFEINDSEKEVNKYLSKKLQLFEFDWLG
                     IIDSNGNFISALKDTGNVKGQDFYRKALQGKETITPVKSQSGTYGILYSVPLYKGDKI
                     VGAVAAWNTIDTMRNLLSVESFHGEGFSQIIDQDGNFIVSSANKNAPKNVVNFFDMIK
                     HQGKQVDKNKLEEMKINLKKNKTGSIYYTLNDGISKAMHYLPLQEENLYLLSVVPTKT
                     ASAQFDLLLKKAIFINILIVILFLLLIILIMFINQKNRAQLIKIAYVDSVTKGYSKAR
                     FDMEAKKIIQSSSSESYSLVSINIQKFKLINDSFGSEAGDKTLKYIHDVISNYLEPGE
                     LLCRVSNDQFNVLIKTIPQKEILKYIENITNDINSFNKNEECKYFIALSIGVYKIDDP
                     NLPLVSIQDRANVARKNNKGNIGNHPLYTCVFYSDLERLKMIKEKEMENRMEEALNNN
                     EFIVYLQPKISLKDNTIVGAEALVRWQDPNKGLIPPDEFIPFFEKNGFITKLDHYVFE
                     KVCSMIRKWIDNGETPIPVSVNFSRANLNNIDFLEKYRMLRNKYNIPAKLLEIELTET
                     LVFENLQKLINIIDQIHEEGFQCSLDDFGSGYSSLNMLKEIKVDTLKLDRAFFSSPNA
                     DNIAENHVIESIVNLAKKLNMNSISEGVETILQMDYLKKINCDMIQGYVFSKPLPQEI
                     FEKMTFGKKLEETSMDSSV"
     gene            complement(8866..10206)
                     /locus_tag="KPD58_001253"
     CDS             complement(8866..10206)
                     /locus_tag="KPD58_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG0265035.1"
                     /translation="MENQEALRHEKIWILLFRYSIPAIIAMMVTSLYNVVDRAFIGSM
                     EGIGSIAIAGLGVTMPVFTLIIAFGMLVSVGASTRLSIKLGERNREEAEKILGNALTL
                     SIIISLIITILGLVFLEEILFILGASKDSIFYAKDYMSVILVGSIFNLVAFSLNNAIR
                     AEGNPKLAARTMIVGCVLNLILDPIFIFVFNLGIKGAAIATVLCQIVVFFWVIHYFIR
                     GKSNLKLKKCNLKLNKDIVKKVFAIGMTPFAMEVAISITHMFTNNSLKVYGGDLAIGA
                     MTALTSILLMFMMPVFGLNQGMQTIISYNYGAKQYERAKKTLILSIIVSVVILSFGFL
                     VVQVFPEVFVGIFNKDSDLMDIAVRGISLNLITLPIMGISIVGPVYFQCISKVKHSMF
                     LTLLRQFILFIPLIIVLPIQFNLDGVWLAQPVADFIAMIIVLLFLKREFKTSRV"
     gene            complement(10218..11000)
                     /locus_tag="KPD58_001254"
     CDS             complement(10218..11000)
                     /locus_tag="KPD58_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0265036.1"
                     /translation="MNCINTKNLNVSYGNTDIIKNLNLNIPKGKITTIIGANGCGKST
                     ILKTIGRIISPNSGKIYINGEDIYKKKSKEIAKDMAVLPQSPQAPSGLTVSELISYGR
                     FPHKSGFGNSSKEDREIVNWSLEVTGIAEFKDRNMEDLSGGQRQRAWIAMALAQETDI
                     LLLDEPTTYLDLAHQLEILKLLDELNKKQGRTIVMVIHELNNAARFADHMIGIKKGKI
                     VCEGDAYDVMTKDNLKELFNIDAEIATDPRTNKPICITYDMV"
     gene            complement(11015..12037)
                     /locus_tag="KPD58_001255"
     CDS             complement(11015..12037)
                     /locus_tag="KPD58_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBG0265037.1"
                     /translation="MKNIIKINSKTLLVISILMVLIFITFLLSLNMGSFSIEPMDVIK
                     TFLGQGQRNHEIAIFKLRLPRIVIALLVGSALSTAGVILQGVTKNDLADSGILGINSG
                     AALFVVVYIYLMNGNVYEGMSNLTVFTMPIVALIGALFGAFLIYSLAWKGGINSSRLL
                     LIGIGVNIAFTSILTIFQLKFTTQEFNRVMAWTSGSIWGTSWKYVIAVLPFIVIFMGI
                     TIYKSKYIDVLNLGDEVSISLGVDVEKQRRRLIIYAVILSGVSTAVAGSISFLGLIAP
                     HIGRKIIGPKHKRLIPVSALIGTLLLLIADTISRNLLAPIEIPVGIVVSIIGVPYFIY
                     LMLVND"
     gene            complement(12043..13056)
                     /locus_tag="KPD58_001256"
     CDS             complement(12043..13056)
                     /locus_tag="KPD58_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBG0265038.1"
                     /translation="MTKLSKKNITYLLILVSIVVLVFGIVLSITIGAKDMNLSTVIDS
                     LIKMEDGINMRIVKDVRLPRAIAAALVGGFLAVSGAIMQGITRNPIAEPSVIGITQGA
                     TFAISISLVLQKKLPQLIHGSFSVMMFAFIGASISGLFIYFISSKSRGRVNNVKLALA
                     GVALGTLLISLASAISMYFNLSQQLSFWISGGLVGVKWEGIKLLFIAGGIGFILAIIM
                     APRITILSLGEEVAIGLGQKTNFVRFICIVLVILMTGASVSVSGNIIFIGLIVPQIAK
                     GIVGSDYKYIIPSSLVLGAVLLVYTDILSRMINPPYETPVGSITALIGVPIFIYLVRK
                     GEK"
     gene            complement(13184..14122)
                     /locus_tag="KPD58_001257"
     CDS             complement(13184..14122)
                     /locus_tag="KPD58_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0265039.1"
                     /translation="MKKIKSLAIFISIITTLVLVTACSDKNTEDKDKSETRVVQSVKG
                     EVKIPSNPKKIVDISGSSEELLLAGYKPVATANVDSYETDKLPSYIREELKGVKIVGH
                     SMMDTMDMEAILEVNPDLIIMSQRQEKIYDQLKEIAPVVMMKDYANDWRSKLTDVSKL
                     FDKEEEAKSWLQKYDEKATKLGKEVIEKNGEKTYLPVLASSGQFMVFSDGGIGTLIND
                     DMKLARPKNMPKQDGITLPMVSMEGLTDIDADHIVVIATESDKKDLENSAIWAQIRAV
                     KDGNVTILDAAPFFSQSYNPIGKELLLESVKNELTK"
     gene            14392..14856
                     /locus_tag="KPD58_001258"
     CDS             14392..14856
                     /locus_tag="KPD58_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265040.1"
                     /translation="MVHLVYCDNAGKKGEKVLDKILAGTKTMVIRGAAGRKIPHSRVF
                     EDELLYFMEKGTAKISAKATVKKVENYVKLTEDEISKILNENQPKLNLSEKQKVRWHK
                     KCLCLVEFENVEEITPLDFDHQGNMDDWLIVEKIEDVVVGTSIPYNYEKSKF"
     gene            complement(14968..15285)
                     /locus_tag="KPD58_001259"
     CDS             complement(14968..15285)
                     /locus_tag="KPD58_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG0265041.1"
                     /translation="MDEKMIEISFGIIGFAGDAKGLAFEAIKEAKSGNIDKARECIEK
                     SKESIHKAHKFQTELIQNEANGNKTEISVILIHAQDHLMTAMNFQQLAEEFIDVYERL
                     ESK"
     gene            complement(15298..16029)
                     /gene="chbG"
                     /locus_tag="KPD58_001260"
     CDS             complement(15298..16029)
                     /gene="chbG"
                     /locus_tag="KPD58_001260"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HBG0265042.1"
                     /translation="MTKIIINADDFGYCEAVNYGIISAHNNGIVRSTSMMANMPGVEH
                     GVGLLKENKTLNCGVHMTLSCGRPLLSNLKTIVDKDGFFIRRITDEIIEKMDCDEIYR
                     ELCAQIDRVKGLGIDISHLDSHHHIHTLVSLKPVIEKIVTKYNLPIRGGFEYNLEYSK
                     VVPLIDSFYKENVSEEYFIKNIDEIMKYDVVDIMSHPAFLDDYILNSTSYAIDRTKEH
                     KILTSKKVKEFLEKNGLVISSYRDI"
     gene            complement(16055..17365)
                     /locus_tag="KPD58_001261"
     CDS             complement(16055..17365)
                     /locus_tag="KPD58_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBG0265043.1"
                     /translation="MKKGLKIVTIGGGSSYTPELVEGFINRYEELPVKELWLVDIEAG
                     KEKLEIVGNLAKRMVKKAGVDMKINLTLDRREALRDADFVTTQLRVGLLDARIKDETI
                     PLSHGVMGQETNGAGGLFKALRTIPVIFDIIKDVEELCPNAWMVNFTNPTGIITEAVF
                     KYTNFRRYIGLCNVPIHLKNDVAKLFNVESDRISMDFAGLNHMVYGLNVSLDGEDVTK
                     EAIDKFVKADISMQNIKAIDFNAEFVKSLGAIPCPYHRYYYKTKEMLEDELREFKEGK
                     ARGQVVKELEEQLFELYKDEKLDVKPPQLEKRGGAYYSDAACNLISSIYNDKKDIQVV
                     NTLNKGSIRDFKDEQAVEVSSVITKNGPKPISIGYLPDSVHGLVSQIKSFEVLAAKAA
                     VYGDYESALLALCINPLIPSDDLAKTILDEMLEAHKDYLPRFNR"
     gene            complement(17462..18775)
                     /locus_tag="KPD58_001262"
     CDS             complement(17462..18775)
                     /locus_tag="KPD58_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0265044.1"
                     /translation="MEKFMSFMDKYIVPVAAKIGAQRHLVAVRDAFIVMIPITMVGAL
                     GTLINNLPLEAYKNLMASIFGENWTTFGGDLWWGAIGTMAVFLVIGVAYFLAKSYESD
                     GLQSGLIALSIFFIMAPQIGKIVPEGGTTVVEGWGMIQQTYLGTAALFSSILIGLLST
                     EIFVRLSKVKKLTIKMPDGVPPAVSRSFAKLIPGMLTIMIFTVIGIFIKMLSNGSFLT
                     DILNTYLGAPLSNVADSLGSTMLIAFIIHILWTVGLHGANIALPFTETILMKLGGENA
                     ALAQAGATEGYHVLAGAFFDAFVYLGGSGMVLGLIVALLIAGRRRKEMIVLGGPPAIF
                     NIGEPLIFGLPIVLNPIFMIPFVLAPVICSAVSYLAIDFGLVAPVILPKIPWVTPPIL
                     GGAMATGDWTGGALALFNLILSILIYIPFVIASEKMEANKLKINN"
     gene            complement(18797..19108)
                     /locus_tag="KPD58_001263"
     CDS             complement(18797..19108)
                     /locus_tag="KPD58_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0265045.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMEDAAKENGIEAKIWAISEVNLKN
                     EIENCDVLLLGPQVRYVLGKATEMAKPYNIPVEVINMMDYGRCNGKAVLDRAVELNSS
                     K"
     gene            complement(19308..20012)
                     /locus_tag="KPD58_001264"
     CDS             complement(19308..20012)
                     /locus_tag="KPD58_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0265046.1"
                     /translation="MKEPIYKVIENHVRELINSDSLKEGDLIPSEKQLSEEFNVTRMT
                     VRSALNNLVKEGYITRQRGVGSIVIANNIYDNISSVSGFTKEMESKGYKVSNILVSLE
                     IVQADENLSSKLNISLEENVWEIKRVRLANDARVSYMITYMPVKLFPNLNKTHCENSL
                     YNFVEEVCGYKIAMSEREVQAVISNEECMDNLKLEEPEPLLYISQICKLQNSEIFEYS
                     HTYHYGYTLTLNAVVE"
     gene            complement(20103..21464)
                     /locus_tag="KPD58_001265"
     CDS             complement(20103..21464)
                     /locus_tag="KPD58_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG0265047.1"
                     /translation="MKDRLNLTEGRITEKLLKLSLPIMGTSFIQMGYNMIDMIWVGKA
                     GSKAVAAVGTAGFFPWLAMAFIMISKVGGEIKVAQSIGANNISTTKSYMKSAIEINII
                     LAIIYTISLIVLNKQLIGFFRLGDLEVITMSRQYLIIVALGMVFYFINPVFTAIFNGL
                     GNSKTPFRINTVGLITNIILDPILIFGWGFAPKLGVAGAAIATVFAQIVVTTCFIYII
                     VKSKEEYFKLRLFKEIETKYYKVLYKLGLPIAIQSGMFTVFSMLLGVIVASWGPVAVA
                     VQKVGSQIEAISWMTAEGLSVALGSFVGQNYGAKKYSRINKGCKIAITVSSVLGIITT
                     LVLVFAGESIFSIFIDESEAIEKGAVYLKILGYSQFFMCLEIITTGAFKGLGRTYIPS
                     IIITILTGARVPLAYFISRPEMLGLNGVWWSITLSSILKGILMISLFISLLKLGKLYK
                     ISE"
BASE COUNT         7729 a         3516 c         2887 g         7609 t
ORIGIN      
        1 acatatgttt tatactaaaa tgttatcaca ttttatctat acatacccat ataactagat
       61 aattaaaatg tataatatta tttgctatag aaaataatac actcatacat aataaatttt
      121 aggagtgaag tatatgaatc aagaatatgt aaaaggaaat cctattttta ctaatattaa
      181 taaagttcca agacaatttc cataccttac tgatgatatt gatactgatg ttataattgt
      241 tggtggtggc gtaactggat gtatctgtgc ttactatcta gctaaaaaca atataaagtc
      301 tgtcattctt gaaaaaggaa ggattgcaca tggaagcact agcgtgacaa cttctctatt
      361 acagtatgaa ttagatgaca atttgataga tttaacagag gttatgactc taaaagatgc
      421 tctaaaagca tataaccttt gtacttcagc acttgaagaa ttagatacat ttatagagct
      481 atatggaaat aagtgtgatt atgccaaaag agatactcta ctttatactg ccaacaaact
      541 tgaagttaag gctatcaaag aagaatataa cttaagaaaa gaaaatggat ttgatgttga
      601 atatattgat gaatccacaa acccttttag ctttgattta aaatcaggat tgatagcaaa
      661 gaatggagga cgagaattag acccatataa atatagtcac catttgattg atgtaagttt
      721 aaaaaatggt cttcaagtat atgaaaatac agaagtaaaa aaagtagatt tatcaaatga
      781 taaggttact gctgaagtat cgtatggata caaagttcat ggtaaaaaat taattgtagc
      841 tacagggtac aatacaagtt tatttacaaa aagaaatttt gcaactaagt caaatacttt
      901 taatatcgca acgaaaccac ttaaaaacat agcttcttgg aaaaacaaca ttttaattag
      961 agataactgt gacccatata attatctaag aactactaaa gacaatcgat taattatagg
     1021 tggtgaagat gtaagttttg atgatataga aaatgaaact ttagccaatg aaaagtacga
     1081 tattttagaa caaagattga aaagtatgtt taaggatata aaagacattg aagttgaata
     1141 taaatattgt ggttgttttg cttcaacttt agataattta ggttttattg gtccagataa
     1201 taaaaataac aatctatggt attgcttagg ttatggagca aatggtatac tctttgcaat
     1261 attaggaggt ataatgttaa gtgaattata tcttggcaaa caaaataaaa atatgaaatt
     1321 atttaaagta gatagatttg ataaatagat attatatatt aaaagactgt aaaaagatga
     1381 aactttataa ttctaaaagt taactttgat acagtctttt ataatgttcc ttttaaaaat
     1441 actttattta taattaaata taaagtaaaa tgttaaaata taactaataa aatttagtca
     1501 taagataata tttaagttta tttgatgaaa tatggaggaa ttattatgaa ggaaatactc
     1561 gttattgctc ccactaaagg aacctatgaa aaatcaattc acatagttaa aaaaaacaaa
     1621 tatacaaata ttgatgttgt atttggcaat ctaaaagaag gtatacctct tgctgaaaaa
     1681 agtattaatc atggtactag aattatcata agccgaggtg gaacttacaa tatgttaaaa
     1741 gctacttaca atatacctat agttgaaatt aaggtagatg cttatgatat tataaaaagc
     1801 tacaaagagg taaaaaattc caacgaacca tttggaataa ttggatttaa taatgtcatc
     1861 tacggtttcg atattataga agaaatattg aataagaaaa taactatgat tgaaattgaa
     1921 aaagaagaag aaatttatga tgctatagaa aagtacagaa agaaaggtat aaacacatat
     1981 ataggagata ccactgttgc acatattgtt aagagactta attgtaaggg aatattaatc
     2041 gaatctagag aagaaaacat acttagagcc attcaacaag cagagcaaat actcgaagca
     2101 accaaggatg aacaaaaaag aagattacag atagaagcta tgacagattt tgtacatgat
     2161 ggaataataa cagttgataa aaattttaaa ataacattat ttaataagag tgctgaaaaa
     2221 atatttggta taaaaaaaga aaatgcactt tatcataatg ttgtagatgt tatcccaaac
     2281 acagttcttc cacaagtagt tgagactatg gaacctcaaa ttggtgaaat acaaaatata
     2341 gggagtacaa aaattattac aaatagagtt ccaatcattg ttgatggtca gattcaagga
     2401 gctgtttcaa catttcaaga tagcaaggag ataagttctt ttgaacatac aataagaagg
     2461 agcttattaa gcaaaggtct atctgctaaa tacacttttg atgatataat tcatgctagt
     2521 gaaaagacaa aagattgtat agaacttgca aaaaaatatg cttgttatga tactcctatg
     2581 attattactg gagaatctgg agttggtaaa gaactatttt gtcaaagtat acataactat
     2641 agtaagcgta aaaatgcacc ttttgttgct gtaaattgtg ctgccatacc tccatctcta
     2701 atagagagtg agttttttgg ttatgaggaa ggttcattta caggtgctag aaaaaaaggt
     2761 aaagcaggtg tattcgaact agcacatgaa ggcacaatct tcctagatga aataagtgaa
     2821 atccctcttg aacttcaagg tcgattattg agggtattgc aggaaaaaca agttatgaga
     2881 attggtggtg ataaagttat tcctatagat gtaaaaatta tctgtgcttc aaataaagac
     2941 ttaaaaaata tgatgcgcaa tggtttattt agacaagacc tttattttag aataaatata
     3001 ctaacattat atcttccttc attaaaagaa agaaaagaag atattataaa attatcagaa
     3061 ttttttatac ataaatattc aagcaaatac aataaaacgc ctttaattat cactccaaat
     3121 attaaaaagg cacttcttga acgagaatat gaaggtaata taagggaatt ggaaggtatg
     3181 atggaaaggg ctgttattgt ggaatcattt gactcgattt tattagaaaa gcttagcaat
     3241 gaatatacaa ataatatagt tgataaagat aatagtgatt ttgaatgcag taatttagat
     3301 ttaaaaactt tagaaaaaaa atatatctat aaaatttata acgaaaataa tagaaatact
     3361 acaaaaacct gtgaaattct aaagataact cgctccactc tatggcgtaa gttaaaagaa
     3421 atagaagatg atgtttaaaa ataatacata tctatatata tcaaaacgtt ttatgtttaa
     3481 ttttaataca taagacgttt atttgttttc aataaaaaat acaattatat gataaaaaga
     3541 atctttgtaa gtgaaattct cgtcaatcat acttttaatt ataagtaatc tacagcattt
     3601 caatcgatta ttaaaaataa tcataataac taaagataca aactctatat attggcatag
     3661 taattgcttt tataatacct atataaaaat gaaagggagt gatactagat gcacatctta
     3721 aaatcagtga aaaagatacc aggaggactt atggtctgtc ccttgttatt tggagcacta
     3781 tttaatacag tatgtccaca agctcttgaa attggaggat tcacaacatc cttatttaaa
     3841 actggtgcaa tgagtatact tgctctattt tgtctatgca atggagctca aatcaatatc
     3901 agacaagctg gtaaaccatt gactaaaggt atagtactta cagcaacaaa atttataatt
     3961 ggtgcagtat taggtattat tgttagtaaa tttattggac ctaaaggtat aattggtatt
     4021 acacctcttg ccatagttgc taccatgact aattcaaatg gaggtctatt tgctgctctt
     4081 gcaggtgaat atggagactc cacagatgtt ggagcaatat ctatcttatc tctaaatgat
     4141 ggtccttttt ttacaatgct tgcttttgga gtaactggac ttgcaaatgt acctatagta
     4201 gcactatttg cagcacttat accaatacta cttggattta tacttggtaa ccttgatgaa
     4261 gatatacgag aatttcttgc acctggtaca acattattaa ttcctttctt cgcttttcct
     4321 ttaggagcag cattaaactt taatcaaatt ataactgctg gactacctgg agttgtactt
     4381 ggtttaggag ttactataat aacaggtatg ggtgggtact ttgtaatgaa acttatacgt
     4441 gcagaacatc cagcagtagg tgcagctaca ggtacaacag ccggaaatgc agctggtact
     4501 cctgaagctt tagcagctat agaccctact ctagctacaa tagcagctgt ttcaacagta
     4561 caagttgctg ctgctattat tgttacagca atatgttgtc caatgttagt ttcctatcta
     4621 gataaaagag aaaaaaagaa aaaatctatt tttaaaacaa ataaaaatat tgaagtaaat
     4681 taatacttaa ggaggtaaaa ttaatgaatg caattattca tgcaaatgaa aaagataatc
     4741 tagttacatg tgtcagacct ttaattaaag gtgaaaaagt ttcaattgat ggaaagtctt
     4801 acatagtaaa tgataatata cctgtctttc ataagatggc tacagaagat gtaaaaaaag
     4861 gtgatgttgt atacaaatac ggtgaaatta taggaattgc aacagttgac ataaaaactg
     4921 gtgactatgt tcatgtacat aacattgaaa gtacacgtgg tcgtggagat aaaaaatagg
     4981 aggaagttaa aatgaaatta atgggttatt taagagaaaa tggtcagttt ggtattcgta
     5041 atcatgtttt aattattcca acatctgtat gttcaagtga gacagctaca agaatagcat
     5101 cacttgtgcc tggagcaata gcaatccctc atcaacatgg atgttgtcaa attggttctg
     5161 atatagaact tacagcaaag acattaatag gatttggaaa aaatcctaat gttgccgcag
     5221 ttttagtagt tggtcttgga tgtgatggta ttcaagcaaa agaattagca tctgaaatag
     5281 ctacaacagg aaaaaaggta gattacgttg ttatacaaga atgtggtgga acattaaaaa
     5341 cagtttctaa gggagctgaa atagtaagta aaatggctcg tgaagtatca aaagaagtaa
     5401 gagttgaatt tggtatgagt gaaattacat tagcacttga atgtggtggt tctgacccaa
     5461 caagtggaat agcttcaaac ccttcaatag gcgttgcatc aaaccttctt gtagatgaag
     5521 gtggaagtag catactaagt gaaactacag aagtaattgg tgcagaacat ttgttggcta
     5581 cccgttttga agatgaagaa atgaaagata agtttcttaa atttgtaagt gatgtagaaa
     5641 aaagagcaat tgctatggga gaagatttac gtagtggtca acctactcca ggcaataaag
     5701 ctggtggact ttcaacaatt gaagaaaagt cacttgggtg tatgtataag gcaggcaata
     5761 agccattcaa aggtgctctt gaatatgctg atattgtacc tcctgataaa aaaggattat
     5821 attttatgga tactccaggt caagatatag attctatcac tggtatggta gcaggtggtg
     5881 cacaaattgt aatcttttca actggtagag gtactccaac aggaagccct atttcaccag
     5941 ttattaaaat cactgggaat agtgatacat acaacaaaat gccagacaat atcgatataa
     6001 atgcaggtag aattataaca gatggtgcta aaatagcaga tataggtcaa gaaatattta
     6061 atgaaataat agaagtctgt aatggtaaac ataccaaagc tgaaagtctt ggtcatagag
     6121 aatttggcat ttacagaatt tcatctacat tttagattgt aacaaaatat cttttataaa
     6181 tataatactt atgtagatac ataagctaaa agttctttct aatcctataa aaattttcta
     6241 tactgaaaag gaactgtatc aaaattattt tgaaaatagt tccttttttc acctctttta
     6301 ctcaaaatat tataaaataa gatttaaaca ataaacgtat cttgattaat ctctaagaag
     6361 acataaataa agctaaatgt aaagtgagat aaataaaatt taatatcttt gtatgaaaag
     6421 atatttggta taatatgtct gagagaattc ttatttgtaa acaaataaag gatggaggat
     6481 gaaacaaaag aatgaacttt gaaaacacaa aaaataaaaa aataatagct actttactat
     6541 tgacactcct tttaatcgtg tcattaacat gtgtcacttt tattatgaat ctaaataaag
     6601 taacacataa tgaaacagaa cgatatcttg tagaagtttc acaaagtatt tccactacaa
     6661 ttaatgtgaa atgtaaaagt atatttagga cactatattc tacgggagat acttattttg
     6721 agattaatga tagtgaaaaa gaagtcaata aatacttatc aaaaaaatta caattatttg
     6781 aatttgattg gttaggtatt atagattcaa atggaaattt tataagtgcc cttaaagata
     6841 caggtaatgt taaaggacaa gacttttata gaaaagccct tcaaggtaaa gaaacaatta
     6901 ctcctgttaa atcacaatct ggtacatatg gaattttata ttctgtacca ctatacaaag
     6961 gtgataaaat tgttggagct gttgctgctt ggaatactat tgatactatg agaaatttat
     7021 taagtgtaga aagctttcat ggtgaaggat tttcccaaat tatcgaccaa gatggaaatt
     7081 ttattgtaag ttcagcaaac aaaaatgctc caaaaaatgt ggtgaatttt tttgacatga
     7141 ttaaacacca aggaaaacag gttgataaaa ataaacttga agaaatgaaa ataaacttaa
     7201 aaaagaacaa aactggttct atatattaca cattaaatga tggtatttct aaggcaatgc
     7261 actatcttcc attacaagaa gaaaatttat acctcctttc agttgttcca acaaaaacag
     7321 caagtgcaca gtttgattta cttctaaaaa aagcaatctt catcaatatt ttaattgtta
     7381 tactattttt attgcttatt atattgatta tgtttataaa tcaaaaaaac agggcacaac
     7441 ttataaaaat cgcctatgta gactctgtaa ctaaaggtta cagtaaagca cgttttgata
     7501 tggaagctaa aaaaattata cagtcatctt catcagaaag ctattcatta gtatctataa
     7561 atattcagaa attcaaactt attaatgatt cttttggtag tgaagcagga gataaaacat
     7621 taaagtacat acatgatgtc attagtaatt atctagaacc aggagaatta ctttgtcgag
     7681 tttctaatga ccagtttaat gtacttatta aaactattcc tcaaaaagaa attcttaagt
     7741 acatagaaaa tatcaccaat gatattaata gtttcaataa aaatgaagaa tgtaaatact
     7801 ttatagctct atctataggt gtatataaaa ttgatgaccc aaatctacct ttagtttcaa
     7861 ttcaagaccg tgcaaatgtt gcacgaaaaa ataataaagg aaacattgga aatcatccac
     7921 tttatacatg tgttttttat agtgatttag agcgtttaaa aatgattaaa gaaaaagaaa
     7981 tggaaaatag aatggaggaa gcattaaata ataacgaatt tatagtatat cttcagccta
     8041 aaataagcct caaagataat actattgtag gtgcagaagc attagtaaga tggcaagacc
     8101 caaataaagg tcttatacca ccagacgagt ttattccttt ctttgaaaaa aatggtttta
     8161 ttacaaaact cgaccactat gtttttgaaa aagtatgcag tatgataaga aaatggatag
     8221 ataatggaga aactccaata cctgtttcag ttaatttttc acgtgctaat ttaaataata
     8281 tagatttttt agaaaaatac agaatgcttc gtaataaata caacattcct gctaagctct
     8341 tagaaattga attaactgaa acacttgtat ttgaaaatct ccaaaaatta attaatataa
     8401 tagaccaaat ccatgaagaa ggttttcaat gttcactaga tgactttgga agtggatact
     8461 cttcactcaa tatgttaaaa gaaataaaag ttgacacatt aaaacttgac cgtgcttttt
     8521 ttagctcacc taatgcagat aatattgctg aaaatcatgt aatagaatct atagttaatt
     8581 tggcaaaaaa attaaatatg aatagtatat cagaaggtgt tgaaacaatc ttgcaaatgg
     8641 attatcttaa aaaaattaat tgtgatatga tacaaggata tgttttttca aaacctctac
     8701 ctcaagaaat atttgagaaa atgacttttg gaaaaaaact agaagaaacc agtatggata
     8761 gttccgttta aataaaataa aaaacttgtc tctataaaat aattttaatc attttataaa
     8821 gacaagtctt tttattttcc aatttaattt tattttctaa atttcttaca ctcggcttgt
     8881 tttaaattcc ctttttaaga ataataatac gattatcata gcaataaaat ctgcaactgg
     8941 ctgtgccaac caaacaccat ctaaattaaa ttgaattgga agtacaatga ttaatggtat
     9001 aaataaaata aattgtctta aaagagttaa aaacattgaa tgtttaactt tacttataca
     9061 ctgaaaatac acaggtccta caatagaaat ccccattata ggcaatgtaa ttagatttag
     9121 gcttatacct cttactgcta tatccatcaa atctgaatct ttattaaata tacctacaaa
     9181 tacttctggg aaaacttgta ctactaaaaa tccaaatgac aatatgacaa ctgacactat
     9241 gatagataat attaaggttt tctttgcacg ctcatattgt tttgcaccat aattgtatga
     9301 tattatagtt tgcatacctt gattcaatcc aaacacaggc atcataaaca tcaataaaat
     9361 agatgtaagt gctgtcatag ctccaatagc taaatctcca ccataaacct ttagagaatt
     9421 atttgtaaac atgtgagtta tacttatagc tacttccata gcaaaaggtg tcattcctat
     9481 tgcaaatacc ttctttacta tatctttatt caattttaag ttacattttt ttagttttaa
     9541 attggacttc cctcttataa aataatgtat tacccaaaaa aatactacta tttgacagag
     9601 tactgttgct atagctgctc cttttatacc taaattaaat acaaatataa atatagggtc
     9661 caatatcagg tttaatacac atcctacaat cattgtcctt gctgctaatt ttggattacc
     9721 ttctgcccta attgcattat taagagaaaa tgcaactaaa ttaaatatac ttccaaccaa
     9781 tatgacactc atatagtctt ttgcataaaa tattgaatct ttacttgcac ccaaaataaa
     9841 aagtatctct tctaagaaaa ctaatcctaa tatagttata attaaagaga taattataga
     9901 taatgtcaag gcattaccta atatcttctc tgcttcttct ctatttcttt cccctaactt
     9961 tatagacaat cttgtgctag cacctactga taccaacatt ccaaaagcta ttattagagt
    10021 aaatactggc atagttacac caagacctgc aatagctata gagccaatac cttccataga
    10081 accaataaaa gctctatcta ctacattata aagtgaagtt accatcattg ctattattgc
    10141 aggtatcgaa tacctaaata ataaaatcca tattttttca tgtcttaatg cttcttgatt
    10201 ctccatgttg tcaccattta caccatgtca taagttatac aaattggttt atttgttctt
    10261 gggtctgttg ctatttctgc atcaatatta aataattctt ttagattatc cttcgtcata
    10321 acatcatatg catcaccttc acaaactatt ttacctttct taatacctat catgtggtca
    10381 gcaaatcttg ctgcattatt aagttcatgt attaccatga caatagttct tccttgtttt
    10441 ttatttagtt catctaataa ttttagtatc tccaattgat gtgccaaatc taaatatgtt
    10501 gtaggctcat ccagtaataa tatatcagtt tcttgtgcca aagccatagc tatccatgcc
    10561 ctttgtcttt gtcctcctga taaatcttcc atatttctat ccttaaattc tgcaattcca
    10621 gtaacttcta aactccaatt tacaatctct ctgtcttctt tactagaatt accaaatcca
    10681 cttttatgag gaaatcttcc ataagaaatt aattctgaaa cggtaagacc actaggagct
    10741 tgagggcttt gtggaagtac agccatatct tttgctattt ctttagactt ctttttgtag
    10801 atgtcctctc catttatata tattttccca ctattagggc taataattct acctatagtt
    10861 ttaagtattg tcgattttcc acaaccatta gcccctatga tggtagttat ttttcctttt
    10921 ggtatattta aatttaaatt ttttataata tctgtgtttc catatgatac atttagattc
    10981 ttagtattta tacagttcat aacaacaccc tttcctaatc atttaccaac attaaataaa
    11041 taaagtacgg cactcctatt atagaaacta ctatgcctac tggtatttct ataggtgcta
    11101 gaagatttct tgatatagta tctgctatta aaagtaataa tgttcctatc aatgctgata
    11161 ctggtattaa tcttttatgc tttggtccta ttatttttct acctatatgt ggagctatta
    11221 aacctaaaaa tgaaatacta cctgctactg ctgtcgatac accagataaa attactgcat
    11281 atattataag tcttcttctt tgtttttcta catctacacc aagacttata gatacttcat
    11341 cacccaaatt taaaacatct atatatttag atttataaat agttataccc ataaaaatca
    11401 ctataaatgg aagcacagct attacatatt tccaactggt tccccaaatg cttcctgatg
    11461 tccatgccat aaccctatta aattcttgtg ttgtaaattt caattggaat atcgtcaaaa
    11521 ttgaagtaaa agctatgttt acacctatac caataagtag taatcttgac gaatttatac
    11581 cacctttcca tgcaagagaa tatataagaa atgctccaaa taaagctcct ataagtgcaa
    11641 caataggcat tgtaaataca gtcaagttac tcataccttc atatacatta ccattcataa
    11701 ggtatatata aactactaca aacaatgcag cacctgaatt tattcccaaa attccagaat
    11761 cagctaaatc attctttgta actccttgaa gtattacacc tgctgttgat aatgcagaac
    11821 caaccaaaag tgctattact attcttggta atcttaattt aaatattgca atttcatgat
    11881 tacgctgacc ctgccctaaa aaagtcttta taacatccat tggttctata gaaaatgagc
    11941 ccatatttaa actcaataaa aaagtgataa aaataagcac cattaaaatt gatataacaa
    12001 gtaatgtttt agaatttatt tttataatat ttttcatagt acctatttct ccccctttct
    12061 aacaaggtat ataaatattg gcactcctat aagtgctgtt atagaaccta ctggtgtctc
    12121 atatggagga tttatcatcc tacttaatat atctgtatat acaagcaata cagctcctaa
    12181 tactaaagag ctcggtatta tatacttata atctgaacct actattcctt tagctatttg
    12241 aggaactata agacctataa aaataatatt accagacact gacacagatg ctccagtcat
    12301 taatattaca agtactatac atataaatct aacaaagtta gttttttgtc ctaatcctat
    12361 tgccacttct tctccaagac ttaatatagt tattcttgga gccataataa tagctaaaat
    12421 aaatccaata cctccagcta taaatagcaa ttttatacct tcccacttca ctcctacaag
    12481 ccctcctgat atccagaaac tcaactgttg agataggtta aaatacatag atattgctga
    12541 tgccaaagaa attaacaatg taccaagagc tactcctgca agtgctagtt ttacattatt
    12601 aactcttcct cttgatttag aacttataaa atatataaat aatccactta tacttgcccc
    12661 aataaatgca aacatcatta cactaaaact tccatgtata agttgaggaa gttttttttg
    12721 aagtaccagt gatatcgaaa ttgcaaatgt agccccttgt gtaattccta taacagatgg
    12781 ttcagctata ggatttctcg ttattccttg cattattgcc ccggaaactg ctaaaaaacc
    12841 tccaacaagt gcagctgcta ttgcccttgg aagtctaaca tcctttacta ttctcatatt
    12901 tattccatct tccattttta tcaagctatc tataacagtt gatagattca tatcttttgc
    12961 cccaatagtt atggataata caattccaaa tacaagtaca actatactta ctaatatcaa
    13021 caaataagtt atattttttt tacttaactt agtcaattat cctacctcct tttccctttt
    13081 aatcgtcagt ataatatcat ctattaagta ttttaatttt aagctttatt aaaaataggt
    13141 gtgaaaaatg aatatcatta tctcacacct actaagttaa gaattacttt gttaattcat
    13201 ttttaactga ttctaataat aactcttttc caattggatt gtatgattga ctgaaaaatg
    13261 gagcagcgtc aagtattgtt acattgccat cttttactgc tcttatttga gcccagatag
    13321 cactattttc taaatctttt ttatctgatt ctgtagcaat tacaactata tggtctgcat
    13381 ctatatctgt taaaccttcc atacttacca ttggtagtgt tattccatcc tgttttggca
    13441 tattcttagg tctagctaat ttcatatcat cattgattag agtacctatt ccaccatcgc
    13501 taaataccat aaattgacct gaacttgcca acactggtaa ataagttttt tctccattct
    13561 tttctataac ttccttacct aactttgtag ctttttcatc atacttctgt agccaacttt
    13621 tagcttcttc ttctttgtca aataactttg aaacatcagt taacttgctt ctccagtcat
    13681 ttgcataatc tttcatcata actacaggag ctatttcttt taattggtca tatatttttt
    13741 cttgtctttg actcattatt attaaatcag gatttacttc taatatagct tccatatcca
    13801 tagtatccat cattgagtgc ccaactatct ttacaccttt aagctcttct cttatgtaac
    13861 ttggtaattt atcagtttca tatgagtcta catttgctgt tgcaacaggc ttataacctg
    13921 ctaataatag ttcttcacta cttcctgata tatcaactat ttttttagga tttgatggta
    13981 tcttaacctc tcctttaaca gattgtacaa ctcttgtctc tgatttatct ttatcctctg
    14041 tattcttatc tgaacatgct gtaacaagta ctaatgtagt tataatagat ataaatattg
    14101 caagtgattt tatttttttc atgattactt ctcctacttg tttattatac ttacatgata
    14161 ttgattatca taagcacata tttattatag catttttcta tttttagtca atattatttt
    14221 gtaaaaggat aaaaattcct aattaatctt aattatatat agcttagata aggttttctt
    14281 atattaaaca gtattttttt aaagaattgg acacaaatac aatgactttt aggttaaaca
    14341 atgataaaat gcaatttata gataatgaat ttagtaagga ggattcaaaa catggtacat
    14401 ttagtatatt gtgataatgc aggaaaaaaa ggagaaaagg tcttagataa aatcttggct
    14461 ggaacaaaaa caatggttat tagaggtgct gctggtagaa aaataccaca tagtagagta
    14521 tttgaagatg agttacttta ttttatggaa aaaggaactg caaaaatatc tgcaaaagcc
    14581 actgttaaaa aggtagaaaa ctatgttaaa ttaacagaag atgaaatttc taaaatttta
    14641 aatgaaaatc aaccaaaatt aaatttatct gaaaagcaaa aagttcgttg gcataaaaaa
    14701 tgtttatgct tagttgaatt tgaaaatgtt gaagaaataa cacctcttga ttttgaccat
    14761 caaggaaata tggatgattg gcttattgtt gaaaaaattg aagatgtagt tgttggtact
    14821 agtatacctt acaattatga gaagtctaaa ttttaaagta tcaataacat aatactatat
    14881 tgctaaactc tacttaaata aaaaaagagt gcatttaata attataaaaa ttatttttat
    14941 acactctttt ttaataataa ttttaaatta cttactttct aatctttcat atacatctat
    15001 aaattcttct gctaattgtt gaaaattcat agctgtcatt aaatggtctt gtgcatgaat
    15061 aagtataaca cttatttcag ttttattacc attagcttca ttttgtatca attctgtttg
    15121 gaatttatga gctttatgaa ttgattcttt cgacttttca atacattctc tagctttatc
    15181 tatattcccg ctctttgctt cttttattgc ttcaaatgct agccctttcg catcacctgc
    15241 aaatccaatt attccaaaag atatctctat catcttctca tccatttatt cgacctccta
    15301 aatatctcta tagcttgata tgactaatcc attcttttct aaaaattctt tgactttttt
    15361 agatgtaagt atcttgtgct ctttcgttct atcaatagca taagatgtag aatttaaaat
    15421 ataatcatct aaaaaagctg ggtgagacat aatatccact acatcatact tcattatttc
    15481 gtctatattt tttataaaat attcttcact tacattttcc ttataaaaac tatctattaa
    15541 aggaactact ttagaatact ctaaattata ttcaaatcca cctcttattg gaagattata
    15601 cttggttaca atcttctcta ttactggctt taaacttact aaagtatgga tatgatggtg
    15661 actatctaaa tgagatatat ctattccaag cccttttacc ctatctattt gagcacaaag
    15721 ttctctataa atctcatcac aatccatttt ttcaataatt tcgtctgtaa tcctcctaat
    15781 aaaaaaaccg tctttatcaa caatagtttt taagttagat aataatggtc taccacaact
    15841 taaagtcata tgaactccac aatttaaagt tttattttct ttaagtaagc ctactccatg
    15901 ttctacccca ggcatatttg ccatcataga agtagaccta actataccat tattatgagc
    15961 tgaaattatt ccatagttca cagcttcaca atatccaaaa tcatctgcat ttattataat
    16021 tttagtcata ccataaccta ctttctatag acaactatct attaaatcta ggaagataat
    16081 ctttatgagc ttccaacatt tcgtctaata tagtttttgc taagtcatca gatggaatca
    16141 atgggtttat acaaagtgcc aaaagagcag attcataatc gccatataca gctgctttag
    16201 ctgccaatac ttcaaatgat ttaatttgac ttactaatcc atgcacagaa tctggtaaat
    16261 atcctatact tattggtttt ggaccatttt ttgttattac actacttact tctaccgctt
    16321 gttcatcttt aaaatctctt atagaaccct tatttaatgt attaactact tggatatctt
    16381 ttttgtcgtt gtatatagaa cttattaaat tacaagcagc atcactatag taagcccctc
    16441 ctcttttttc aagttgaggt ggttttacat ctaatttttc atccttatat aattcaaata
    16501 attgttcttc caattcttta actacttgac ctctagcttt tccctcttta aactctctca
    16561 actcatcctc aagcatttct ttagttttat agtagtatct atgatatgga caaggtattg
    16621 cacccaaaga ttttacaaat tcagcattaa aatcaattgc ctttatattt tgcatactta
    16681 tatctgcttt gacaaattta tcaattgctt cttttgtaac atcttctcca tctaaagata
    16741 catttagacc ataaaccata tgattaagac ctgcaaaatc catacttatt ctgtcacttt
    16801 ctacattaaa tagtttggca acatcatttt ttaaatgaat tggcacgttg catagaccta
    16861 tatatcttct aaaattagta tatttgaata cagcctctgt tattattcct gttggatttg
    16921 taaaattcac catccatgca tttggacata attcttccac atcttttatt atatcaaata
    16981 taactggtat tgtgcgaagg gctttaaata aacctccagc accattagtt tcctgcccca
    17041 taactccatg agataaagga atagtttcat cttttattct cgcatctaaa agacctactc
    17101 taagttgtgt tgtcacaaag tctgcatctc ttagagcttc tcttctatct aaagtaagat
    17161 ttattttcat atctactcca gcttttttta ccatcctctt agctagattt ccaactatct
    17221 ctaatttttc ttttccagct tctatatcta caagccataa ttcctttaca ggtaactctt
    17281 catatctatt tataaaaccc tctacaagct caggagtata acttgaacct cctccaattg
    17341 taactatttt tagtcccttt ttcatctcat tataccatcc ttacctataa tttaatatac
    17401 tctacaaaac cccatacatt tatttaaaac tctatacatt tatttaccga aataaaacta
    17461 cctagttgtt aattttcaat ttattagctt ccattttttc agacgctatg acaaatggaa
    17521 tatatattaa tattgataat attaaattaa atagtgctaa agctccacca gtccagtcac
    17581 ctgtagccat tgctccacct aatataggag gtgttaccca aggtatttta ggtaaaatta
    17641 ctggtgctac taatccaaaa tctattgcta agtatgatac agcactacat attactggtg
    17701 caagtacaaa tggaatcata aatataggat ttaaaactat tggaagtcca aatatcaatg
    17761 gttctcctat gttaaatatt gcaggtggac cacctaatac tatcatctct ttacgtcttc
    17821 ttccagctat taataaagct actattaatc ccaataccat tccagaacca cctaaatata
    17881 caaatgcatc aaagaatgca cctgcaagta catggtatcc ttccgttgct cctgcttgag
    17941 ccaaggcagc attttctcca cctaatttca taagtatagt ttctgtaaaa ggaagtgcta
    18001 tatttgcacc atgaagtcca acagtccaaa gtatatgaat tataaatgca ataagcattg
    18061 tagaacctaa actatcagct acatttgata gtggtgctcc taaataagta tttaaaatat
    18121 ctgttaagaa acttccatta gaaagcattt ttataaatat tcctattaca gtaaatatca
    18181 ttatagttaa cattcctggt attaactttg caaatgatct tgaaactgct ggtggaactc
    18241 catctggcat ttttatagtt aatttcttta ccttagataa tcttacaaat atctcagttg
    18301 ataataatcc tattaatata gaagagaata aagctgctgt acctaagtaa gtttgttgta
    18361 tcattcccca tccttcaact actgtagttc ctccctcagg aactatttta ccaatttgtg
    18421 gagccattat aaaaaagata gataaagcta taagcccaga ttgtaaccca tcactctcat
    18481 aagattttgc tagaaaataa gcaacaccta ttactaagaa tactgccatt gtacctattg
    18541 ctccccacca aaggtcgcct ccaaatgtgg tccagttttc tccaaatata cttgccatca
    18601 agtttttgta tgcttccaat ggtaagttat ttatcaatgt tcccaatgca cctaccatag
    18661 ttataggtat cattactata aatgcatctc ttactgctac taagtgcctt tgagctccta
    18721 ttttagcagc tacaggcact atatatttat ccataaatga catgaatttt tccattttac
    18781 atcctccagt acaactttat tttgaactat ttaactctac agctctatct aacacagctt
    18841 ttccattaca tcttccataa tccatcatat ttataacttc tactggaata ttatatggtt
    18901 ttgccatttc agttgctttt cctaatacat atctaacttg aggccctaaa agtaagacat
    18961 cacaattttc tatttcattt tttaagttca cttctgatat agcccatatt tttgcttcta
    19021 taccgttttc ctttgctgca tcctccattt ttgtaactaa taaactagtt gacattcctg
    19081 ctgaacaagc taacattatt tttatcattt tttcccctcc aaattagatt cattaaatat
    19141 tttttaagtc ataagttttt taaactatac atttaaaata tgcttttcta ttaatttttt
    19201 cttaatgtat atatcttatt tttaaatgta tatacattta attcttgata ttattatata
    19261 ccaaatgtat atacatttca agaaaacttt ttcattttat ttaaatttta ttcgacaaca
    19321 gcattcaaag ttaatgtata tccatagtga tatgtgtgtg agtactcaaa aatctcacta
    19381 ttttgtaact tacaaatttg gcttatatat aaaagtggct ctggttcttc taattttaga
    19441 ttatccatac attcttcatt cgatattaca gcttgtactt ctctctctga catggctatc
    19501 ttgtatccac acacttcttc aacaaaatta taaagagaat tttcacaatg agtcttattt
    19561 aaatttggaa ataacttaac tggcatataa gtaatcatat atgaaactct tgcatcatta
    19621 gctaatctta ctctttttat ttcccaaaca ttttcttcta aacttatatt caacttactt
    19681 gataaatttt catctgcctg aactatttca agagatacca atatattaga tactttatag
    19741 cctttacttt ccatctcttt agtaaatcca cttacagacg atatattatc atatatatta
    19801 tttgcaataa ctatactacc tacacctctt tgtctagtta tatagccttc tttaactaga
    19861 ttattaagtg cagaccttac agtcattcta gttacattaa attcttcact cagctgtttt
    19921 tcagaaggga ttaaatcccc ttctttcaaa ctgtctgaat ttattaactc tctaacatga
    19981 ttttctataa ctttataaat tggctctttc atttaattca ctccttatta aatcttttat
    20041 ataataagat aattttatat acaaacaaag aaaatgtcta tacatttaag ataatttaaa
    20101 ttttattcag aaatcttgta caactttccg agttttaata aagatataaa tagacttatc
    20161 attaatatac cttttaatat acttgataag gttatactcc accatacacc atttagaccc
    20221 aacatttctg gtctagatat aaagtaagcc aatggtactc ttgcaccagt cagtatagta
    20281 attataatag aaggtatata ggttctccct aatcctttga aagctcctgt tgttattatt
    20341 tctaaacaca taaaaaattg agaatagcct aatattttaa gataaactgc tcctttttct
    20401 attgcctcac tttcatctat aaaaatagaa aatatactct ctcctgcaaa cactaatact
    20461 aaagtagtta ttataccaag tacagaagat acagttatag ctattttgca acctttattt
    20521 attctagaat acttcttggc accataattt tgtccaacaa aactgcctaa tgcaactgat
    20581 agcccttcag ccgtcatcca ggaaattgct tctatttgtg agcctacttt ttgtactgca
    20641 acagccacag gcccccatga tgctactatt actcctaata gcattgagaa tactgtaaac
    20701 ataccacttt gtattgctat aggtaatccc aatttataca gaactttata atatttagtc
    20761 tctatttctt taaatagtct taatttaaaa tattcttctt tacttttaac tattatatat
    20821 ataaaacatg tagtaactac tatctgagca aatacagttg caatagcagc tcctgcaaca
    20881 ccaagttttg gagcaaatcc ccatccaaaa attaatatag ggtcaagtat aatattagtt
    20941 attaatccta ctgtattaat acgaaaagga gttttactat ttccaagacc attgaaaatt
    21001 gcagtaaata ctggattaat aaaataaaat accataccta gagctactat tatcaaatac
    21061 tgtctagaca tggttataac ttctaagtct cctaatctaa aaaaacctat caattgttta
    21121 ttaagtacta ttaaagaaat tgtatatatt attgctagta taatatttat ttctattgct
    21181 gacttcatat atgatttagt agtgcttata ttatttgcac caatactttg tgctactttt
    21241 atctctccac ctacttttga aatcataata aatgccattg caagccatgg aaaaaaacca
    21301 gctgttccaa ctgcagctac tgctttactt ccggctttac caacccatat catatctatc
    21361 atgttatacc ccatctgtat aaatgaagtc cccattattg gaagtgatag cttaagtaat
    21421 ttttctgtaa ttcttccttc tgttaaatta agtctgtctt tcattttcca cctcttttat
    21481 attaaattta tttgcttata tagctagatt aatcatttta tttgattgag ttcactatcc
    21541 ttcaatttat attatcatat ctcaggattg catctcaatc atacttatgt ttaagataca
    21601 gtattttacc tatctatgat aaaattaata cttatgaata taaatctagc catttcatca
    21661 atacatattc taacctttaa cttaacttca attaagtata catatcgtaa ccaattagaa
    21721 tattttatac ggtatcttcc a
//
LOCUS       DAELLV010000040        21328 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.040, whole genome shotgun sequence.
ACCESSION   DAELLV010000040 DAELLV010000000
VERSION     DAELLV010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21328
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.040"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(182..376)
                     /locus_tag="KPD58_001266"
     CDS             complement(182..376)
                     /locus_tag="KPD58_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265048.1"
                     /translation="MFQVINFLCLFCVGFLVLDVMIYFCKDAMDIFEGTTNVNKVKSI
                     NVWADRVRANSYSYENKIAK"
     gene            complement(685..2214)
                     /gene="lysS"
                     /locus_tag="KPD58_001267"
     CDS             complement(685..2214)
                     /gene="lysS"
                     /locus_tag="KPD58_001267"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBG0265049.1"
                     /translation="MKNNQQSNEEAQIQEDLSEVLQVRRDKLKKLQESGRDPFKESRY
                     DRTHYSMDIKDNFDSLEGKTTKIAGRIMSKRIQGKAGFIDIQDQEGRIQSYVRLDAIG
                     EEEYSVFSTYDIGDIVGIEGEIFKTKKGEISVKAKSVVLLCKSLQVLPEKYHGLKDQE
                     LRYRQRYVDLIVNPEVKNAFLIRTKALKALRAYLDDRGFLEVETPILNTIAGGANARP
                     FITNHNTLHIPMYLRIANELYLKRLIVGGFDKVYEMGRMFRNEGMDLKHNPEYTAIEL
                     YQAYADYTDMMEITENVIAHMAEVATGSMIVNYQGTEINFTPPWKRMSMEDCVKEYSG
                     VDFSTINTDEEALEVAREKGIEIKPGMRRGEVINAFFEEFGEDKLIQPTFITHHPVEV
                     SPLSKRNVEDPRRTDRFEAFANKWELANAFSELNDPIDQKGRFIDQLRKRELGDDEAF
                     EMDEDFLKALEVGLPPTGGLGIGIDRVIMLLTNSPSIRDVLLFPTMKPIDNNPNKEEE
                     N"
     gene            complement(2229..2708)
                     /gene="greA"
                     /locus_tag="KPD58_001268"
     CDS             complement(2229..2708)
                     /gene="greA"
                     /locus_tag="KPD58_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBG0265050.1"
                     /translation="MEENKEFLLTQEGYDKLEEELENLKVVKRKEVAERIKVAISFGD
                     LSENAEYDEAKKEQAQVEERILKLENMVRKAVIIDESKIDLNVVTIGSIVKVKDLEFD
                     EEVEYTIVGSTEADPYDGKISNESPVGKALLGRAAKEVVEVQVPDGVAKFEILEIRR"
     gene            complement(2817..3785)
                     /gene="dusB"
                     /locus_tag="KPD58_001269"
     CDS             complement(2817..3785)
                     /gene="dusB"
                     /locus_tag="KPD58_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBG0265051.1"
                     /translation="MKIGNLELKNKVFLSPMAGVTDLPFRLICKEQGCGLLYTEMING
                     KALCYDDENTKKMLKIEEEEHPVAVQIFGSEPEFMGRAAEIMNDYSNEILDINMGCPA
                     PKVVKNGDGSALMKNPKLAEEVLRAVVKNSKKPVTLKIRKGWDDNSVNAVEIAKIAED
                     CGISALAIHGRTREQFYTGKADWDIIAEIKKNLSIPVIGNGDVFTIEDSINMLDKTGC
                     DAIMIGRGAQGNPWIFKRINHYMNTGEILPEPTLNEKISTAIKHLKLAVEEHGEYVAV
                     REMRKHIAWYLKGLRNSAKLRDEINKIEDYQEVVSKLEYYMEDSLT"
     gene            complement(3922..4692)
                     /locus_tag="KPD58_001270"
     CDS             complement(3922..4692)
                     /locus_tag="KPD58_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HBG0265052.1"
                     /translation="MLLVFDVGNTNMVLGIYKGDKLVNYWRIKTDREKTSDEYGILIS
                     NLFDYDNVNISDIDDVIISSVVPNVMHSLENFCIKYCKKQPLIVGPGIKTGLNIKYDN
                     PKQVGADRIVNAVAGIEKYGAPSILVDFGTATTFCAISEKGEYLGGTIAPGIKISSEA
                     LFQSASKLPRVELAKPGMTICKSTVSAMQSGIIYGYVGLVDKIISMMKKELNCDDVKV
                     IATGGLAKLIASETKSIDYVDGFLTLEGLRIIYEKNQE"
     gene            complement(4711..5301)
                     /locus_tag="KPD58_001271"
     CDS             complement(4711..5301)
                     /locus_tag="KPD58_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG0265053.1"
                     /translation="MMNTSASVRKVNVRKMTIIGVLSAISIMLSMTPLGFIPVGPTKA
                     TIMHIPVIIGAIMEGPIVGAAIGFIFGISSLLNAITNPTVTSFVFINPLVSILPRVMI
                     GVLAYYVYQLIIKATNKVYISGLITGAIGSLLNTAGVLGMIYVLYADKYLHAMGKSGS
                     AGKVIMALAAANGVPEAIVGALVVAAVATVLKKSKK"
     gene            complement(5484..6452)
                     /locus_tag="KPD58_001272"
     CDS             complement(5484..6452)
                     /locus_tag="KPD58_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HBG0265054.1"
                     /translation="MYNNILDIEGLKVGQVEDRNGLTGCTVVICEEGATCGVDVRGSA
                     PGTRETDLLDPVNMVQKVHAVVLAGGSAYGLESTCGVSRYLEEKGIGFDVGVGKVPIV
                     VGAVLFDLGVGSFKCRPDLKMGYRACEVANTDILNQGNYGAGCGATVGKIRGQEYAMK
                     GGIGSYSIKLDNGLVVSALIAVNALGDVYENGKVIAGALNDKKTETIDTYKLMKQGES
                     KGGFSVDNTTIGIIATNAKLTKAGCKKISQMGHDGYAKSIFPIHTPHDGDTIFTISTG
                     EIEADITLVGSLAVEVVEKSIINAVKKADSVENIMAYKDIVKKREI"
     gene            complement(6445..7422)
                     /locus_tag="KPD58_001273"
     CDS             complement(6445..7422)
                     /locus_tag="KPD58_001273"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HBG0265055.1"
                     /translation="MRDKIIEIILNNKSEFISGEELSKQLGVSRAAIWKHMKALKEEG
                     YNIESVNKKGYRLAENPTDLLSPQNIYYKLDTEFIGKNIEHLDTIDSTNEHAKRIAIN
                     SVDGTVILSEHQDMGKGRLGRRWDSKPHEGIWMSIILKPNMEPFKAPFITLIAGASVT
                     KALNNLGANTLIKWPNDVILNGKKICGILTELSAEIERTNYVVLGIGINVKIMDFEQE
                     IADKATSLYKEGYMLSRVDIVRNVLTEFEKLYLDYTVNNSKEKTLKICRDYSAIIDKN
                     IYVLKGDNRELVKCLDINEEGNLLVQRSDGSIKEIISGEVSIRGEKGYV"
     gene            complement(7737..9707)
                     /gene="ftsH"
                     /locus_tag="KPD58_001274"
     CDS             complement(7737..9707)
                     /gene="ftsH"
                     /locus_tag="KPD58_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBG0265056.1"
                     /translation="MNKLLKGAGFYLLVFIIIVGIVQFSGKPTEKIKDLKFSEVYREL
                     TDENISRLYFVNQTSVEGTIKDTNTKFKSYVPTEVMGNKLADEVLDQAKAGKLTFGGE
                     AKPSTPWFVEMLPTLLLIFFMVIIWFVFMNQSQGGGGKVMSFGKSKAKVHKDDEKTRV
                     TFKDVAGLDEEKEDLQEVVDFLKNPKKYIELGARIPKGMLMVGPPGTGKTYLSRAVAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKSAPAIIFIDEIDAVGRKRGAGLG
                     GGHDEREQTLNQLLVEMDGFGVNQGIIIMAATNRPDILDPALLRPGRFDRQVVVGTPD
                     VKGREAIFKVHSRNKPLSDDVKMDVLARRTPGFTPADIENLMNEAAILTARKREKKIK
                     METIEEAITKVIAGVAKKSKVISEKERRLTAYHEGGHAVCAHVLEEVSPVHQVTIVPR
                     GRAGGFTMQLPVEDKFYATKNEMKENIVVLLGGRVAEELVLKDVSTGASNDLERVTAT
                     ARSMVTKYGMSSKLGPMSFDSDDEVFLGNSFSSKRNYSEEVAFEIDQETKRIVDGAYD
                     KTRSILQENMDRLEYVAQALLIYETLDAEQFVKAFNKELPLNEIENAVTEENSSKEVE
                     EQLTIKLEKDEEERNNVIDINKNLEDKSDKDK"
     gene            complement(9741..11129)
                     /gene="tilS"
                     /locus_tag="KPD58_001275"
     CDS             complement(9741..11129)
                     /gene="tilS"
                     /locus_tag="KPD58_001275"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBG0265057.1"
                     /translation="MIFDKVLSTINKHNLIQKGDKIVLGLSGGPDSVCLLHVLNRLKK
                     DFNIEIYAAHLNHQIRGIEAQKDALYVSKLCEDMGIIFFVKSINVPKYCENEGLSLEE
                     GARKLRYEMFYEIKDKIKANKIAIGHNLNDQAETVMMRIMRGTGLKGLKGIDYIRDNC
                     IIRPILDVERNEIEEYCEAYNLNPRIDKTNLENIYTRNKIRLDLLPYMKDNFNSNVIE
                     SIVRMSNSLKSDNDYIEKEAEAKFREVSNIKEKGFVEINLDDFVCLHDAIKVRVLRNS
                     IKHILGDTNFVDQRHIEDIMSLEDNSKVNKMLTLPRNIFVYRKKDSIILTNEEIVNEE
                     IEFYYNVPSNGFIKIKELKQIIETQVMSIDRYKSMKLDNSSKGFDFNKVKGGIVIRSR
                     RQGDKIKLAMGSKKVKDLFIDLKIPREERCKIPIITDSEGIICVGDYKISENYKIDEN
                     TKEVLKINFNKL"
     gene            complement(11312..11740)
                     /locus_tag="KPD58_001276"
     CDS             complement(11312..11740)
                     /locus_tag="KPD58_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HBG0265058.1"
                     /translation="MEEKSLSVKISINTRQYDEKGNMDTIEMTAFGKIFYKNDGIYVI
                     YKEKEEDIEITNTIKILKNQVSIKKFGAINSTMLFKCGESSTTKYVTPQGTLLIDIDT
                     RELDINIQEGEHIKLKIDYNIKVQDLFLGRNKIDIYIGTK"
     gene            complement(11757..12563)
                     /locus_tag="KPD58_001277"
     CDS             complement(11757..12563)
                     /locus_tag="KPD58_001277"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBG0265059.1"
                     /translation="MNNKPIGVFDSGLGGLTVLKEIMKILPNEDIIYFGDTARIPYGS
                     RSKETIIKYTFQAINFLKTKGVKAIVIACNTATARSLKEAQEKYDIPIIGVIEAGART
                     AVSSTKNKIVGIIGTEGTISSKAYNLEISKIDESIEIVNKACPLFVPIVEEGWANTEV
                     AKLTAKIYLQELKEKNIDSLVLGCTHYPILKRTIGEEVGEHIKLVNPAKETAKDLKKI
                     LEVQNIINNTEIHGTYQYYVSDIPEKFSDIAREFLKKKIDKIQKVEIQKY"
     gene            complement(12765..13250)
                     /locus_tag="KPD58_001278"
     CDS             complement(12765..13250)
                     /locus_tag="KPD58_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HBG0265060.1"
                     /translation="MATLLALTFIFMPIKEVFAYEPTENIKKVNKQEKNIAKKEVINL
                     SDNDLMLLSKLVAGEARGESYEGQVAVAAVVINRVLDPRFPDTIEEVIYQKNAFSVVL
                     DGSIKMAPTDSARKAAQEALYGTDPTNKAVYFWNPEIATCKWINRLNPYMRIGNHVFA
                     K"
     gene            complement(13301..13960)
                     /gene="spoIIR"
                     /locus_tag="KPD58_001279"
     CDS             complement(13301..13960)
                     /gene="spoIIR"
                     /locus_tag="KPD58_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HBG0265061.1"
                     /translation="MRKIKIRLGLLILSLISVISIMTIVINGEVKKVDNISKDYKDKL
                     IRFHVIANSNTDEDQELKLKVRDEVIKYLQPKLQNSKSIKESEAIIKKEYSSLEEISK
                     NIILKNGYNYSVKVGIQYSNFPTKQYSNIVLPAGEYKALKIIIGKGEGKNWWCVMFPP
                     LCFVDESNGVIDKSTDDKLKEVLTDKEYKLIKQDTPKKTSRVKIKFKVLEVVKDLEKK
                     F"
     gene            complement(14105..14785)
                     /locus_tag="KPD58_001280"
     CDS             complement(14105..14785)
                     /locus_tag="KPD58_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0265062.1"
                     /translation="MENLTKLNLDNYKPLRDVVFENLREAILEGKLKPGQRLMEVQLA
                     EQLGVSRTPVREAIRKLELEGLVVMLPRKGAYVANMSLKDIMDVLEVRASLEGLAAYL
                     AAERISDEDIKKLKDISEEFKKSTLESDVDALLKLDVEFHECIFKATNNKKLIQLINS
                     LWEQVYRFRVTYMSAYDTSHNIVEEHKYLLDAIIKGDSELSKKYATEHIEKAEQFMID
                     KAMISKDL"
     gene            complement(14769..15659)
                     /locus_tag="KPD58_001281"
     CDS             complement(14769..15659)
                     /locus_tag="KPD58_001281"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBG0265063.1"
                     /translation="MDFIRLKSRAKINLSIDVLGKRQDGYHFVEMIMQTIDLYDIVKI
                     KELDEDEIKVKSTSLDIPLDEDNIVYKAAKILKNKFYIKKGVEIFIEKNIPVAAGMAG
                     GSSNAAAVLVGLNHLWELRLSEDELKEIGLNLGADVPFCISGRPALAQGIGEKLTKIK
                     GLPCDTNILICKPDLFVSTKEVYQGLDLNNIKKRPNNKYLIECLKSEDIKAVSESMVN
                     ILENVTIGKHKEISDIKQVMMKNNALGSMMSGSGPTVFGLFKNKEDALIGKKELLKKY
                     KQVYVVNSSQKGVEICGEFN"
     gene            complement(15777..17327)
                     /locus_tag="KPD58_001282"
     CDS             complement(15777..17327)
                     /locus_tag="KPD58_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HBG0265064.1"
                     /translation="MELIKDVIKVDNRIDFGKFQTFIEAEAVVPDKKLDVYEIVKTEG
                     YISLKKIEMADGKILCRGSFNYNVIYIADDKNTISNVDGKVDINEVIEKDNVVQDMEY
                     MLFSEVEHMDCTIMNERKIKVGALMNIRGSLFEKQRLDIVKDVAQVEGIQKHRKEICF
                     QDIVGIEKAESSIRDTITINTEEIQSIISLNPCVKVKESRVTDNKVIIGGVLEINPLA
                     CTYEGELVELDRVGIEFTQFVEVPGVSDGMTEEVLLSMSDFNHIFKQNSESNTGLLEI
                     DCMACCKVKVTDEVTREVLQDAYSPQKIIKFDHKPIQLNKTLRRSEETFMVREGIRND
                     NDDIQIKDIVSVCPTMSIENSYIEGNKSIIQGIIKVEILFVPVEGLKLVYKISEEIPF
                     EHDIEMDNLTDTASVFNTACIDKVEVDLNRDQIDLIIKIKRFTEALDKKAENFIIKGE
                     DQGVYDLSKAPSIIVYICKEGDTFWNIAKKYNTTENEIAELNDIKLDEPIKPGKCLIL
                     EKKVVLVD"
     gene            complement(17421..17687)
                     /locus_tag="KPD58_001283"
     CDS             complement(17421..17687)
                     /locus_tag="KPD58_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HBG0265065.1"
                     /translation="MATVQTLDKIRVSLERHIGKKILLKANKGRKQIITKEGILEKVY
                     PSVFVIKLDDESNGYPRVSYSYSDLLTSNVKLQVFRDQDKLQIS"
     gene            complement(17835..18695)
                     /gene="yabG"
                     /locus_tag="KPD58_001284"
     CDS             complement(17835..18695)
                     /gene="yabG"
                     /locus_tag="KPD58_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HBG0265066.1"
                     /translation="MKVGDIVARKSHNKDIVFKIVSFGVDENNEKIAILKGIAFRVIA
                     DAYIDDLELVKAPDIKDILIDKNVENLLYKSVRKAKERQKRMTRAVPKLQLNPNTYGM
                     PGKVLQIDGDKEYLKICLDVYAQLGIPAVGVAVSEQNQYKEVRALLEKYNPDILVITG
                     HDAMTIKRGDIEDMSNYRNSSNFVKTVKEARKWQPNLDNLVIFAGACQSNYEKIISAG
                     ANYASSPGRIMIHALDPVFIVEKIACSRIDVVVPIDEVIEQTITGVKGIGGSETRGKF
                     RWAMPKTILH"
     gene            complement(18779..19717)
                     /locus_tag="KPD58_001285"
     CDS             complement(18779..19717)
                     /locus_tag="KPD58_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HBG0265067.1"
                     /translation="MKFIDLHCDTIAKLMENVETSELKSNKYSVDIDRLKKGDSLAQT
                     FALFVDTEEVKHPFDYCMSMANKFHEEMKKNSDEIALATNYEEIMKNQSEGKLTALLS
                     IEEGAVLEGKLENLKKFYDLGVRMMTISWNHVNELSFPHNKVEYREKGLTDFGREVVH
                     KMNELGMLVDVSHISDGGFYEIAKISSKPIIATHSNSRAMMNHSRNLTDDMIKVLANK
                     GGVTGINFFHLFLSDKSESKLEDMVRHIKHIVNVGGIDVVSLGSDFDGIDSKVEIEDI
                     SQMGKLYEPLKKEGFSEDDIEKIYYKNALRVIKEVL"
     gene            complement(19827..19988)
                     /locus_tag="KPD58_001286"
     CDS             complement(19827..19988)
                     /locus_tag="KPD58_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265068.1"
                     /translation="MFILRFADDDDNLSVKDFTSLTDLKKYISENNIDKTWHQIEEVK
                     KVIPNLKEE"
     gene            complement(19998..20495)
                     /locus_tag="KPD58_001287"
     CDS             complement(19998..20495)
                     /locus_tag="KPD58_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HBG0265069.1"
                     /translation="MSLYTEWRNLSDNHESQEAEVKFWEEYLKVEAGIYNEILNNKMD
                     VVEGKVSELAEKFGTTNEFFMGFIDGISESLKEDIVLEEVEADKEVSIKIDWEKLYNN
                     MVTVEAHWLYNLQGWEDILPADQRKEIIKAYKKSKTIVKEAKIGRNDSCPCGSGKKYK
                     KCCGK"
     gene            complement(20610..21320)
                     /locus_tag="KPD58_001288"
     CDS             complement(20610..21320)
                     /locus_tag="KPD58_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0489 family protein"
                     /protein_id="HBG0265070.1"
                     /translation="MENYEYSGFYIEKPVGNNVFSYDKRENKSIYVPKLINGTLNDVR
                     LGNEVVFNEVDENKEIKAKGLENMVEYVLGNKKIYVFDNHNHAFYFWAKSLLKGEFTK
                     GCKLVHVDQHKDTREPENYDVDVNNLKDVFRYTNEVLNVGSFIKPALKYNIFSELIII
                     DSLYGFDLEVESEFVLDIDLDIFSSDMDYIPFELKFYKIKNLIKKAKVITIATSPYFI
                     NQEYAIKVLKELFNYDII"
BASE COUNT         6366 a         4122 c         2080 g         8760 t
ORIGIN      
        1 ttatgtttgc ccttttctta aggacaagta atactatatc atcttaaaaa tgtttcgtca
       61 atactttttt tgttttttat tttaaaaaag gttataactt ttggtttttt taataaatat
      121 aattaaaact aatagatata acccttgata ttacaaacaa tacatgcata tataatttta
      181 cctattttgc aattttattt tcataagaat atgaattagc tctcactcta tctgcccaaa
      241 catttatact cttaacttta ttaacattag ttgtaccctc aaaaatatcc attgcatcct
      301 tacaaaaata tatcataaca tcaagaacca aaaatccaac acaaaataaa cataaaaaat
      361 taattacttg aaacatcaga cattccccca ataatttttt atatatagta ttctcataaa
      421 ttattctttt taccttctca taaattattc taaatatttt cataataaat tactttcaat
      481 catattttct aaatataaag tctcaatcaa caaaaactaa tatcaatttc taacgtttgt
      541 tggtaattta taaattaaat atctaaaaga atctttgaag catataaaaa gcattccaca
      601 ctgacatttt aaataaaatt tagctattta ttgataattt aatagctaaa ctttatttaa
      661 aacttagtag aatgctaata tttcttagtt ttcttcttct ttatttggat tgttatcaat
      721 aggtttcata gtcgggaata ataatacatc tcttatagat ggagagttag ttaataacat
      781 aattactcta tctataccta ttcctagtcc tcctgtagga ggtagaccta cctctaatgc
      841 ttttaagaaa tcttcatcca tttcaaaagc ttcatcatca cctaattctc tttttctcaa
      901 ttggtctata aatcttcctt tttggtcaat agggtcatta agttcagaaa atgcattagc
      961 taactcccac ttattagcaa atgcctcaaa tctatcagtt cttcttgggt cttcaacatt
     1021 tctcttagat aatggagata cttcaactgg atggtgagtt ataaatgttg gttggattaa
     1081 tttatcttct ccaaattctt caaagaaagc atttattact tctcctcttc tcattccagg
     1141 ttttatctct ataccttttt ctctagcaac ttctagtgcc tcttcatctg tatttatagt
     1201 agaaaaatct acaccagaat attctttaac acaatcttcc atactcattc ttttccaagg
     1261 tggtgtaaag tttatttctg ttccttgata gttaactatc atacttcctg tagcaacttc
     1321 agccatatgt gcaataacat tttctgttat ttccatcata tcagtatagt ctgcatatgc
     1381 ttgatataat tctattgcag tatattcagg attatgtttt aaatccatac cttcatttct
     1441 aaacattctt cccatttcat aaactttatc aaatccacca acaataagtc tttttaaata
     1501 caattcattc gctattctta aatacattgg tatatgtaaa gtattatgat tagttataaa
     1561 tggtctagca tttgccccac ctgctatagt atttaatatt ggagtctcaa cttctaaaaa
     1621 tcctctatca tctaagtatg ctcttaatgc ttttaatgct tttgttctaa ttaaaaaagc
     1681 attttttact tctggattta ctattaagtc aacatatctt tgtctatatc ttagttcttg
     1741 gtcttttaat ccatgatatt tttctggtaa aacttgtaaa gatttacata gtaatactac
     1801 agatttagcc tttacagata tttcaccttt tttagtttta aagatttctc cttcaattcc
     1861 aactatatct ccaatatcat atgttgaaaa tacactatat tcttcctctc ctatagcatc
     1921 tagtcttaca taagattgga ttcttccctc ttggtcttga atatctataa agccagcttt
     1981 tccttgtatt ctcttgctca ttatacgtcc tgctattttt gtagttttac cttcaagaga
     2041 atcaaagtta tcttttatat ccatagaata atgagttcta tcatatctac tttctttaaa
     2101 aggatctcta cctgactctt gtaatttttt tagtttatct cttcttactt gaagtacttc
     2161 actaaggtct tcttgaattt gcgcttcttc attgctttgt tgattatttt tcattttttt
     2221 cctccaaatt atcttcttat ttctaatatt tcaaacttag caactccatc aggaacttgc
     2281 acttccacaa cttctttcgc agctctacct aaaagtgctt tccctacagg agattcgttt
     2341 gatattttac catcatatgg gtctgcttct gtcgaaccaa ctatagtata ttcaacctct
     2401 tcatcaaatt ctaaatcctt aactttaact attgatccta ttgtaacaac attaaggtct
     2461 attttgcttt catctatgat aacagctttt ctaaccatat tttctaattt taaaattctt
     2521 tcttcaactt gtgcttgttc ctttttagct tcatcatact cagcattttc agataaatca
     2581 ccaaaagata tagcaacttt tattctctct gcaacttctt ttctctttac aacctttaag
     2641 ttttctaatt cttcttctaa tttatcatat ccctcttgtg tcaataaaaa ctctttattt
     2701 tcttccatta taaatacaac tccttcacaa atttcttagg aattttccta attatatttt
     2761 aaaaatttta taatcttttt ttaatttatg cgctatatta taagtcacta ttatttttat
     2821 gtcaaggagt cttccatata gtactccaat ttcgacacaa cttcttgata gtcttctatt
     2881 ttgttaattt catcccttaa ttttgcagag tttctaagtc cttttaaata ccaagcgatg
     2941 tgttttctca tttcccttac agctacatac tctccatgtt cttctactgc aagttttaaa
     3001 tgcttaatgg cagtacttat tttttcgtta agagttggct ctggcaaaat ctcgcctgta
     3061 ttcatataat ggtttattct tttaaatatc caagggttac cttgagcacc cctacctatc
     3121 attatagcat cacaaccagt tttgtctaac atatttattg aatcttctat tgtaaataca
     3181 tcaccatttc ctataacagg tatactcaaa tttttcttaa tctctgcaat tatatcccag
     3241 tctgctttgc ctgtataaaa ctgttctcta gttcttccat gtattgctaa agcacttatt
     3301 ccacaatcct ctgctatctt tgctatttca acagcattta cactattatc atcccagcct
     3361 tttcgaatct ttaaagtcac tggctttttt gagttcttca ctactgccct caatacctct
     3421 tctgcaagct taggattttt cataagtgct gagccatctc catttttaac tacttttgga
     3481 gctggacacc ccatatttat atcgagaatc tcatttgaat aatcattcat tatctctgct
     3541 gctcttccca taaactctgg ttctgaaccg aatatctgaa ctgctacagg atgttcttcc
     3601 tcttctatct taagcatttt tttagtattt tcatcatcat agcaaagtgc cttaccattt
     3661 atcatttctg tatataaaag cccacatccc tgttctttac aaattaatct aaatggaagg
     3721 tctgttacac ctgccatagg tgatagaaaa actttatttt tcaattctaa atttcctatt
     3781 ttcattgttt ttctcctatt tttgccttta ttttgtgatt taactttaaa ttcttatttc
     3841 ctcattataa tataccacat atagctagaa atctatcata aaaagggaat tggatatata
     3901 acccaattcc ctgtatatta attattcttg gtttttttca tatattattc tcaatccttc
     3961 tagtgttaaa aaaccatcta cataatctat acttttcgtc tctgaagcaa tcagtttagc
     4021 taatccacct gtagctataa ccttaacatc atcacaattc aattctttct tcatcatact
     4081 tattattttg tcaactaaac caacatatcc ataaattatt ccagattgca tggctgatac
     4141 agtactctta caaatagtca tacctggctt agctaattct actctaggta atttagacgc
     4201 actttgaaat aacgcctcac tagatatttt tattcctggt gctattgttc cacccaaata
     4261 ttcacctttt tcagagatag cacaaaatgt agttgctgtt ccaaaatcaa caagtatact
     4321 tggtgctcca tacttttcta tccctgctac agcattaact attctatctg ccccaacttg
     4381 ttttggatta tcatatttta tatttagacc tgtttttatg cctggaccta ctattaatgg
     4441 ctgtttttta cagtacttta tacaaaagtt ttcaagagaa tgcataacat tcggaactac
     4501 agatgatatt ataacatcat caatatcact tatattcaca ttatcataat caaataggtt
     4561 acttatcagg attccatatt catcagacgt tttttcccta tctgttttaa ttctccagta
     4621 attaactaat ttatcacctt tatatatacc taaaaccata ttagtatttc caacatcaaa
     4681 tactagaagc atatgtcatt aaccttcttt ctattttttg cttttcttaa gtacagtggc
     4741 aacagcagct actactaaag cccctactat tgcttctggc accccatttg ctgcggcaag
     4801 agccattatt acttttcctg ctgaaccact tttacccatt gcatgaagat acttgtcagc
     4861 atataagaca tatatcattc ctaaaactcc agctgtattt aacagagaac ctattgcccc
     4921 tgtaattaaa cctgatatgt ataccttatt tgtagcctta attattaact gatatacata
     4981 ataagctaat acacctatca taactcttgg taatattgaa actaatggat ttataaaaac
     5041 aaaagatgtt actgttgggt ttgttatagc atttaagaga cttgatatcc caaatataaa
     5101 tcctattgct gccccaacta ttggtccttc cataatagct ccaataataa ctggtatgtg
     5161 cattatagtt gcttttgtag gtccaactgg aataaatccc aatggagtca tagatagcat
     5221 aatagaaata gctgatagta ctcctattat agtcatcttt cttacattta cttttcttac
     5281 tgatgcactt gtgttcatca taaaaaatac acctccgttc cagctcatta agatgccgga
     5341 ctacttaatt ttttaatttt ttcgtttttt tcttataatt atgagtcaag ctaccattgt
     5401 aggatgcccg ctctaacaag aataatttta tcaagatgag ttgccattgt caacaatttg
     5461 acaagaaaaa tatgctaatt tcatcatatt tctcgttttt ttactatatc tttatatgcc
     5521 attatatttt ctacactatc cgcttttttt actgcattta ttatactttt ttccacaacc
     5581 tctacagcca atgaacctac taatgttata tctgcttcta tttctcctgt tgatattgtg
     5641 aatattgtat ctccatcatg aggtgtatgg ataggaaaaa tagacttagc atatccatca
     5701 tgtcccattt gagatatctt tttacatcca gctttggtta acttagcatt tgttgcaatt
     5761 atcccaattg tagtattatc tacactaaat ccacctttac tttcaccttg tttcattaat
     5821 ttatatgtat caattgtttc tgtcttttta tcatttaaag caccagctat aaccttacca
     5881 ttttcataaa catctccaag agcatttaca gcaattaatg cagatacaac tagaccatta
     5941 tctaatttta tagaatagct acctattccg cctttcatag catattcctg acctcttatc
     6001 tttccaacag tggctccaca acctgctcca taatttcctt gatttaaaat atcagtattt
     6061 gcaacttcac aagctctata acccatcttt aaatctggtc tacatttaaa gctccctact
     6121 cctaaatcaa ataaaactgc tcctacaact ataggcactt tacctacacc tacatcaaaa
     6181 ccaattcctt tttcttctaa gtatctactt acaccacaag tggattcaag accatatgca
     6241 gaacctccag ccaaaactac tgcatgaact ttttgaacca tatttacagg gtctaataaa
     6301 tcggtttctc tagtccctgg agcagagccc cttacatcaa caccgcaagt agctccttct
     6361 tcacatatta ctactgtgca cccagtaaga ccatttctat cctcaacctg cccaaccttt
     6421 aagccttcta tatccaaaat attattatac atatcctttt tctcctctta tagaaacttc
     6481 acctgaaata atttctttta tacttccatc acttctttga actaataaat ttccctcttc
     6541 atttatgtct aaacatttca ctagttctct attatctcct tttaatacat atatattttt
     6601 atctataatt gcagaataat ctctacatat ttttaaggtt ttttctttgg aattatttac
     6661 agtataatct aaatataatt tttcaaattc agtcaataca ttcctaacaa tatctactct
     6721 ggataacata tacccttctt tatacaatga tgtggctttg tcagctattt cttgttcaaa
     6781 atccattatt ttaacattta ttccaattcc aagaacaaca taatttgttc tttcaatttc
     6841 agcagataac tctgttaata taccacatat tttttttcca tttaaaataa catcatttgg
     6901 ccattttatt aatgtatttg ctcctagatt gtttaaagct ttcgttacac ttgctcctgc
     6961 tattaaagtt ataaatggag ctttgaatgg ttccatattt ggttttaata taatactcat
     7021 ccagattcct tcatgaggtt ttgaatccca tcttcttccc aatcgtcctt tccccatgtc
     7081 ttgatgttca cttaaaatca cagtcccatc tacactattt atagctatcc ttttagcatg
     7141 ttcatttgtt gaatctatag tgtcaagatg ttcaatattt tttccaataa actctgtatc
     7201 taatttatag taaatattct gtgggcttaa taaatcagtt ggattttccg ctaatcgata
     7261 ccctttttta tttacagatt ctatattata tccctcttct tttaaggctt tcatatgttt
     7321 ccatatagct gcacgtgaca cccctaattg ttttgataat tcttctcctg aaataaattc
     7381 agatttatta tttaatataa tctctattat tttatctctc atatttcacc tctaatatga
     7441 cataatttca tgacttattt tcttctaata aataattata cattattaat atacttttaa
     7501 tactattaat tttaaatttc ctttttaatt attataatgg ttgttttata ttttatattg
     7561 atgatttata taattttaca ttttatttaa ataattttaa aataataaaa aaactgaaag
     7621 tatcaattaa attaatacat gcaaatataa tttgcttatt aatttaatat tatactttca
     7681 gttttagtat ttaatccaca tcagataata atcttaaaat gaccaaggtc ttaaaactat
     7741 ttatctttat cagatttatc ttctaaattt ttatttatat caataacatt gtttctttct
     7801 tcttcatctt tttctaattt tatagttaat tgttcttcaa cttctttaga agaattttcc
     7861 tctgtaactg cattttcaat ttcattcaga ggtaactctt tattaaatgc ttttacaaat
     7921 tgttctgcat ctaaagtttc atatataagt aaagcttgtg ctacatactc taatctatcc
     7981 atattttctt gtaatatact tcttgtctta tcatatgcac catcaactat acgctttgtt
     8041 tcttggtcta tctcaaaagc aacttcttca gaataatttc tcttacttga aaaactatta
     8101 cctaggaata cttcatcatc actgtcaaat gacattggac caagtttaga actcattcca
     8161 tactttgtta ccatacttct tgcagtagct gtaactcttt ctaagtcatt tgaagcacct
     8221 gtagatacat ccttaagtac tagctcttct gctactctac caccaagtaa aactacaata
     8281 ttttctttca tttcattttt tgtagcatag aacttatctt caacaggaag ttgcattgta
     8341 aaccctccag ctcttcctct tggaactatt gtaacttgat gaacaggact tacttcttca
     8401 agtacatggg cacaaactgc atgccctcct tcatggtatg ctgtaagtct tctttctttc
     8461 tcacttatta ctttcgattt tttagccaca cctgctataa cctttgttat agcttcttct
     8521 atagtttcca tcttaatttt cttttctctt tttctagctg ttaatattgc agcctcattc
     8581 attaagttct ctatatcagc aggagtaaat cctggtgttc ttctagccaa gacatccatt
     8641 tttacatcat cacttaatgg tttatttctt gagtgaactt taaatatagc ctctctacct
     8701 tttacatctg gtgtaccaac aacaacttgt ctgtcaaatc ttcctggtct aagtaaagct
     8761 gggtcaagta tatctggtct atttgtagct gccatgatta ttataccttg atttacccca
     8821 aatccatcca tttcaactag aagttgatta agagtttgct ctctttcatc atgacctcca
     8881 ccaagacctg cacctctttt tctaccaact gcatcaattt catctatgaa tatgatagct
     8941 ggagcacttt tcttagcctg ttcaaataaa tctcttactc ttgaagcacc aaccccaaca
     9001 aacatctcaa cgaaatcaga accacttata ctgaaaaagg gaactcctgc ttctcctgca
     9061 actgctcttg atagatatgt tttaccagtt ccaggaggtc ctaccataag cattcctttt
     9121 ggtattcttg cacccaattc aatatatttc ttaggattct ttaagaaatc tactacctcc
     9181 tgcaaatctt ctttttcctc atctagtcca gctacatctt taaatgtaac tctagttttt
     9241 tcatcatctt tatgaacctt agcttttgat ttgccaaaac tcataacctt tccgcctcca
     9301 ccttgagact gattcataaa cacgaaccaa ataatcacca tgaagaatat taacagtaat
     9361 gtaggcaaca tttcgacaaa ccaaggtgta gatggcttag cttcaccacc aaaagttagt
     9421 ttcccagcct tagcttggtc taacacctca tctgcaagtt tatttcccat cacttctgtt
     9481 ggaacatatg acttaaactt tgtatttgta tcttttatag taccttctac agaagtttga
     9541 tttacaaagt ataatcttga tatattctca tctgttaatt ctctatatac ttcagagaat
     9601 tttaaatctt ttattttttc tgtaggctta cctgagaatt gaactatacc tactataatt
     9661 ataaaaacaa gtagataaaa acctgcccct tttagcaact tattcaatat agaagccctc
     9721 ctttcataca ttgctttgcc ctataatttg ttaaaattaa tttttaatac ttcttttgtg
     9781 ttttcatcaa ttttataatt ttcactgatt ttataatctc caacacatat tattccttca
     9841 ctatctgtga ttataggaat tttacatctt tcttctcttg ggatttttaa atctatgaat
     9901 aaatctttca cttttttact tcccatagca agctttatct tatcaccttg ccttctgctt
     9961 cttattacta tacccccttt taccttatta aaatcaaacc ctttagatga attgtctaat
    10021 ttcatactct tatacctatc tatgctcatt acttgggtct caataatttg ttttaattct
    10081 tttattttaa taaacccatt actaggtaca ttgtaataaa attcaatttc ctcattaaca
    10141 atttcctcat tcgttaatat tatactgtct ttttttctat aaacaaatat atttcttgga
    10201 agagttaaca ttttatttac ttttgaatta tcttctaaag acattatatc ttctatatgt
    10261 ctttggtcaa caaaattagt atctcctagt atatgtttta tggaatttct aagaactcta
    10321 actttgatag catcatgtaa gcaaacaaaa tcgtctaaat ttatctctac aaagcctttt
    10381 tcttttatat ttgaaacttc tctaaattta gcttctgcct ctttctcaat ataatcgtta
    10441 tcacttttta aactattgct catcctcact atagattcta ttacatttga attaaaatta
    10501 tccttcatat atggtaaaag gtctaacctt atcttatttc tagtgtatat attttctaaa
    10561 tttgttttat ctatcctcgg atttaaatta taagcttcac aatactcctc tatctcattt
    10621 ctttccacgt ctaatatggg tctaattatg caattatctc taatataatc aattcccttt
    10681 aaacctttta aacctgttcc tctcatgata cgcatcataa ccgtttcggc ttggtcattg
    10741 agattatgac ctattgctat tttattggct ttaatcttat ccttaatttc ataaaacatt
    10801 tcatatctta gcttccttgc tccttcttct aatgataatc cctcattttc acaatatttt
    10861 ggtacattaa tagactttac aaagaatata attcccatat cctcacaaag tttagataca
    10921 tataatgcat ctttttgagc ttctatccct cttatttgat ggtttaaatg tgctgcatat
    10981 atttctatat taaaatcttt ttttaatcta tttagtacat gcaataaaca aactgaatca
    11041 ggtcctccag aaagacctaa aactatttta tcgccttttt gtattaaatt atgtttattt
    11101 atagtactta gtaccttatc aaatatcata tttatccctc ttacacttag ttttattcac
    11161 ttttaagtat actttctata aaaaaacgct aaacccttgt atcaatttta agaaattaaa
    11221 gtttaatata aatataataa taccaaataa attaaataaa aggtagtttt ttcactacct
    11281 ttataaaatt tctggaaaat agccatagtt attatttagt acctatgtat atatctattt
    11341 tattcctacc taaaaataaa tcttgtacct ttatattata gtctattttc aattttatgt
    11401 gttccccttc ttgtatattt atatctaatt ctcttgtatc aatatcaatt aataaagtac
    11461 cttgtggggt cacatacttt gtagtactac tttctccaca tttaaacagc attgttgagt
    11521 taatagcacc aaactttttg atactaactt gatttttgag tatctttata gtattagtta
    11581 tttctatatc ttcttctttt tccttgtaaa taacatatat tccatcattt ttataaaaaa
    11641 ttttaccaaa ggcagtcatc tctatggtgt ccatatttcc tttttcatca tattgtctag
    11701 tatttatact tatttttaca ctcaacgatt tttcctccat agattcttca tataaactag
    11761 tatttttgta tttctacttt ttgaatctta tcaatttttt tctttaagaa ctctcttgct
    11821 atatctgaaa atttttcagg tatatctgat acataatatt gataagttcc atgaatttct
    11881 gtattgttta taatattttg cacctctaaa atctttttta aatcctttgc agtctcttta
    11941 gctggattaa caagttttat atgctctcca acttcttcac ctattgttct ctttaaaata
    12001 ggataatgag tacatcctaa cactaaagaa tctatatttt tttctttcaa ttcttgtaaa
    12061 tatatctttg ctgtcaattt agccacttct gtatttgccc atccttcttc aacaataggc
    12121 acaaataaag gacatgcttt attaacaatt tctatactct catctatttt agatatttcc
    12181 aagttgtatg cttttgaact tatagttcct tcagttccta ttattcctac aattttgttt
    12241 tttgtagaac ttacagctgt tctagctcct gcttctataa ctccaattat cggtatatca
    12301 tatttttctt gagcttcttt taagcttctt gcagtagcag tgttacatgc tataactata
    12361 gcttttacac cttttgtttt taaaaagttt atagcttgaa aagtatattt aattatagtt
    12421 tctttggatc ttgaaccata tggtattctg gcagtatctc caaagtatat tatatcctca
    12481 tttggtaata tcttcattat ctcttttaga actgttaatc cacctaatcc cgaatcaaaa
    12541 actcctattg gtttattgtt catctttata caacacctac tttacatttt tgtgtttaac
    12601 aacaacttat ttatcgctaa aatttatatt tatattcata attaatatat tattacctaa
    12661 atgattatta tagtttattt tacacaaaaa ataaaggagc tatcttaaat tagaaaataa
    12721 tttctacaaa aagacaactc ctttcactac ttaaaaattt aaatttattt tgcaaacaca
    12781 tgatttccta ttctcatata aggattcaat ctatttatcc atttgcaagt ggctatttct
    12841 ggattccaaa aatatactgc tttattagtt gggtcagttc cataaagagc ttcctgagct
    12901 gctttacgtg ctgagtctgt tggtgccatc tttattgaac catcaagcac aactgagaaa
    12961 gcattttttt gatatatgac ttcttctatt gtgtctggaa atctagggtc caatactcta
    13021 tttattacta cagctgctac agcaacctgt ccctcatagc tttctcctct agcttctcct
    13081 gcaaccaact tagataataa catcaagtca ttatcagata aatttattac ttctttcttt
    13141 gctatatttt tttcttgttt gtttactttc ttaatatttt ctgttggttc atatgcaaat
    13201 acctctttaa taggcataaa tataaaagtc aaagctaata gtgtagccat cagtttactc
    13261 cacctgtcca tgattaaaac cactccttac attcttattt ttaaaatttt ttctctaaat
    13321 ccttgactac ttcaagtact ttaaacttaa tttttactct acttgttttt tttggtgtat
    13381 cttgttttat taacttatat tctttatcag ttaaaacttc cttcaactta tcatctgtag
    13441 atttatctat cactccatta gattcatcaa caaaacatag tggagggaac ataacacacc
    13501 accagttttt tccttcacct ttcccaatga taatctttaa tgctttatat tcaccagctg
    13561 gaagaactat attagaatat tgtttagtag ggaaattgct atattgtata cctaccttta
    13621 cactgtaatt atatccattt ttaagaataa tatttttact tatttcttcc agactactat
    13681 attccttttt tataatagct tcactttctt ttatactttt tgaattttga agctttggct
    13741 gtaaatattt tattacttca tctctaactt ttagtttaag ttcttggtct tcatctgtat
    13801 tgctgtttgc tataacatga aatcttatta atttatcttt ataatctttt gatatattgt
    13861 ctactttttt tacctcacca tttattacta ttgtcattat tgatatgact gatataagac
    13921 ttaatattaa tagtcccaat ctaatcttaa tttttctcat tgaaaattcc ccctcaagat
    13981 aaatttatat catcaattat gtccaattaa atttaattta ttcataatat tttttaatac
    14041 taataaaaaa agctatctta aaatgattac aatcatttta aaatagcttt ttaaacctta
    14101 tattttataa gtctttacta atcatagcct tatctatcat aaactgctct gctttttcta
    14161 tatgctctgt agcatacttc ttggatagct cactatcacc tttaattata gcatcaagta
    14221 agtatttatg ctcttcaaca atattatgtg atgtatcata agcagacata taggtaactc
    14281 taaatctata tacttgttcc cacaatgagt ttattaattg gattaatttt ttattattag
    14341 ttgctttaaa tatacattca tgaaattcta catcaagttt taataacgca tctacatcgg
    14401 attccaaagt gcttttttta aattcttctg atatatcttt aagcttttta atatcttcat
    14461 cactaattct ttcagcggca agatatgctg ctaagccttc taggcttgct cttacttcta
    14521 atacatccat aatatccttt aatgacatat ttgccacata cgcacctttt ctaggcaaca
    14581 taacgactaa accttctaat tcaagctttc ttattgcctc tctcactgga gtcctactta
    14641 ctcctaattg ttctgctagt tgcacttcca ttagcctttg accaggcttt aattttcctt
    14701 ctaagattgc ttctcttaaa ttctcaaaga ctacatctct taatggcttg taattatcca
    14761 agttcagttt agttaaattc tccacaaatt tcaacccctt tttgactgct attaacaaca
    14821 tacacctgtt tatatttttt taataattct ttttttccaa ttagagcatc ttctttattt
    14881 ttaaatagtc caaaaactgt aggaccactt ccactcatca tagaacctaa agcattattt
    14941 ttcatcatta cttgttttat atcacttatt tctttgtgtt tacctatagt aacattttca
    15001 agaatattta ccatgctttc tgatacagct tttatatctt cagattttaa acactctatc
    15061 aaatatttat tatttggtct ttttttaata ttattcaaat ctaatccttg ataaacttct
    15121 ttagtagaaa cgaataagtc tggtttgcat atcaatatat ttgtatcaca tggtaaacct
    15181 ttgatttttg ttagcttttc tccaatacct tgagctaagg caggtcttcc tgatatacaa
    15241 aaaggaacat ctgcgcccaa attaagtcct atttctttta attcatcttc agataatcta
    15301 agttcccata ggtgatttaa acctactaat actgcggctg cattagaact tcctccagcc
    15361 atccctgctg ctacaggaat atttttttct atgaatattt ctacaccttt ttttatataa
    15421 aatttatttt taagtatttt ggcagcttta taaacaatat tatcttcatc taaaggaata
    15481 tctaaactag tgctttttac ctttatttca tcttcgtcta attcttttat ctttactata
    15541 tcgtataaat ctatcgtttg cattatcatt tcaacaaaat gataaccgtc ttgccttttt
    15601 cccaagacat caatggataa atttattttt gctctactct ttagtcttat aaaatccatt
    15661 ctatcctcct tgtttaaaga ataatgcatc actaacaata atattatact acaaaaaata
    15721 aaataatagg gtattataat accctattat acttaattac tttaaatttt ttaatattaa
    15781 tctactaata cgactttttt ttctaaaata agacacttac caggcttaat tggttcatca
    15841 agttttatat cattaagttc tgcaatttca ttttctgttg tattgtattt ttttgctata
    15901 ttccagaaag tatctccttc tttgcatata tatacgatta tgctaggagc tttagataaa
    15961 tcatatacac cttggtcttc tcccttgata ataaaatttt ctgctttctt atctaatgct
    16021 tcagtaaatc tcttaatttt gataatcaag tctatctggt ccctattcaa atcaacttcg
    16081 actttatcaa tacatgctgt attaaacaca gatgctgtat cagttagatt atccatttct
    16141 atatcatgtt caaatggaat ttcctcacta attttataaa ccaattttaa accttcaact
    16201 gggacaaata aaatttcaac ttttatgatt ccttgtataa tgcttttatt tccttcaata
    16261 taactatttt ctatagacat ggttggacaa acgcttacaa tatcttttat ttgtatatca
    16321 tcattatcat ttcttatgcc ttctctaacc ataaatgttt cttcactacg tcttaaagtc
    16381 ttatttaatt gaataggttt gtggtcaaat tttattatct tttgaggcga atatgcatct
    16441 tgtaatactt ctcttgtaac ttcatctgta acttttacct tacaacaagc catacaatct
    16501 atttctaaaa gacctgtgtt gctttcacta ttttgcttga aaatatgatt aaaatctgac
    16561 atagacaaca atacttcttc agtcatacca tcactaactc cagggacttc tacaaattga
    16621 gtaaactcaa tacctactct atccaactct acaagttcac cctcatatgt acaagctaac
    16681 ggatttattt ctaatactcc accaataata actttattgt cagtcactct actttctttt
    16741 actttaacac atggatttaa acttattata gattgaattt cctctgtgtt tattgttata
    16801 gtatctctta tagaactttc tgctttttct attccaacta tatcttgaaa acaaatttcc
    16861 tttctgtgct tttgtatgcc ttccacttgc gccacatctt ttacaatgtc taacctttgc
    16921 ttttcaaaca aacttcccct tatattcata agagctccta ctttaatctt tctctcattc
    16981 attatagtac aatccatatg ctctacttct gaaaataaca tgtattccat atcttgaaca
    17041 acattgtctt tttctattac ttcgtttata tctacttttc cgtctacatt agaaatagta
    17101 tttttatcat ctgcaatata gattacatta taattaaaac tacctctgca aagtatcttc
    17161 ccatctgcca tttcaatttt tttaagtgat atgtatcctt ctgtttttac aatttcatat
    17221 acatctaatt ttttgtctgg tacaactgcc tccgcttcaa tgaatgtttg aaattttcca
    17281 aagtctattc tattgtcaac tttaattaca tctttaatta attccatagt ctacctcctt
    17341 aagtattata aaaatatcac actatattta tatttaagga gcaaaaaaaa aattacacct
    17401 atttttaaat aggtataatt ttaacttatt tgtaatttat cttgatctct aaaaacttgc
    17461 aactttacat tagatgttaa taaatctgag taactataag atactctagg atatccatta
    17521 ctttcatcat ctaactttat aacaaacacg cttggataaa ctttttctag tataccctct
    17581 tttgtaataa tctgctttct tcctttatta gctttcaaca atattttctt tcctatatgt
    17641 ctctctaagc ttactcttat cttatctaga gtttgaacag tagccacaat atcaccttct
    17701 ttaacgttta tatgtttaca taatatcaca tataaacatt tttgtcaaat gtttaaacta
    17761 attattttat atattttttg caaaaatgtc aatactattt ttgaagatat acagtataat
    17821 tatgttaatt tttgctaatg taatattgtt tttggcattg cccatctaaa ttttcctctg
    17881 gtttcagaac caccaattcc cttaacacct gtaatagtct gctctataac ctcatcaata
    17941 ggaactacta catcaattct tgaacaagca attttctcca ctataaaaac agggtctaat
    18001 gcatgtatca ttatccttcc aggcgaactt gcatagtttg ctcctgcact tattattttt
    18061 tcataatttg attggcatgc tcctgcgaat atgacaagat tatccaaatt tggttgccat
    18121 ttacgtgctt cttttactgt tttaacaaaa tttgaagagt ttctatagtt actcatatct
    18181 tctatatctc ctctttttat tgtcattgca tcatgacctg ttattactag tatatccgga
    18241 ttatattttt ctagtagtgc tcttacttct ttatactgat tttgctctga aacagctaca
    18301 cctactgcag gtattccaag ttgagcatat acatccaagc aaatttttaa atattcttta
    18361 tctccatcta tctgtagtac ttttccaggc attccatatg tgttaggatt taattgaagt
    18421 tttggaactg ctctagtcat tcttttttgt ctttctttag cttttcttac tgacttatat
    18481 aataaatttt ctacattttt atctattaat atatctttta tatcaggtgc ttttactaat
    18541 tctaggtcat caatataagc atctgctatt actctaaatg caattccctt tagtatagct
    18601 attttctcat tattttcatc tacaccaaaa gagactattt taaatactat atctttgtta
    18661 tgtgactttc tagctacaat gtctcctacc ttcatagtta aacctccaat aaatttttat
    18721 cttagtttat actatgttcg atataaaact ttgtttccta atgtcaaaaa aacatttctt
    18781 ataatacttc ttttatcact cttaaagcat ttttatagta tatcttttct atatcatctt
    18841 cactaaaccc ttcttttttt agaggttcat acagtttacc catctgagat atatcttcaa
    18901 tctcaacttt tgaatctatt ccatcaaaat ctgaccctag agatacaaca tctattcctc
    18961 caacatttac aatatgtttt atgtgtctaa ccatatcttc aagtttactt tcacttttat
    19021 cacttaaaaa taagtggaag aagtttatac ctgtaactcc acctttattt gctaatactt
    19081 taatcatatc atcagttaaa tttctagaat gattcatcat tgctcttgaa ttagaatgtg
    19141 tagcaattat tggtttagaa gaaatctttg caatttcata aaatccacca tctgaaatat
    19201 gagaaacatc aaccaacatt cccaattcat tcatcttatg taccacttct cttccaaaat
    19261 cagtaagccc tttttctcta tattctactt tattatgagg aaagctcaat tcattcacat
    19321 gattccaact tattgtcatc attctaaccc ctaagtcgta aaatttcttt aaattttcta
    19381 atttaccttc aagtactgca ccttcctcaa tagataaaag agctgtaagt ttaccttctg
    19441 attggttttt cataatttct tcatagttag tagctaaagc tatttcatca ctattttttt
    19501 tcatttcctc atgaaattta tttgccatac tcatgcaata atcaaaagga tgttttactt
    19561 cttcagtatc aacaaaaaga gcaaatgttt gagctaatga atcccccttt ttcaatctgt
    19621 ctatatcaac agaatattta ttagacttaa gttcacttgt ttctacattt tccattaatt
    19681 ttgctatagt atcacaatgt aaatcaataa atttcatatt tataccccct gtatatttta
    19741 tatttatatc taaaaataga aatagttgtc cttatagaca actattgaca actatttttt
    19801 aatattcttt ttaatattac ttaatcttat tcttctttta agttaggtat tactttttta
    19861 acttcttcaa tttgatgcca tgttttatct atattatttt cactaatata tttttttaaa
    19921 tcagttaaag atgtaaaatc tttaactgat aaattgtcat catcatctgc aaatcttaaa
    19981 ataaacatga gactccctta ctttccacaa catttcttat atttttttcc gcttccacat
    20041 gggcatgaat catttcttcc tatttttgct tctttaacta ttgttttaga cttcttataa
    20101 gcctttatta tttcttttct ttggtctgct ggtagtatat cttcccaacc ttgtaaatta
    20161 tatagccaat gagcctcaac agttaccata ttgttatata atttttccca atctattttt
    20221 atagaaactt ctttatcagc ttctacttct tctaaaacta tatcttcttt taagctttca
    20281 cttattccat ctatgaatcc catgaaaaat tcattagtag ttccaaattt ttcagccaat
    20341 tcacttactt taccttcaac aacatccatt ttattattta atatttcatt gtatattcca
    20401 gcttcaactt tcaaatattc ttcccagaat tttacttctg cttcttgact ttcatgatta
    20461 tcacttaaat ttctccattc agtatataaa ctcatacttt ttcctcctaa gtatacctat
    20521 atttttatag tttttatagt ataaaatctt ttagttagat tggaagtttc ttctttttga
    20581 ttataccata ttctatttaa agttaactct tatattatat cataattaaa caattctttt
    20641 aatactttta tagcatactc ttggtttata aaataagggc ttgtcgcaat tgttattact
    20701 ttggcttttt taattaagtt ttttatcttg taaaatttga gttcaaatgg tatatagtcc
    20761 atatcacttg aaaatatatc caaatctatg tcaagaacaa actctgattc tacttctaaa
    20821 tcaaatccat atagactatc tattattatt aattctgaaa atatgttata ttttaaagca
    20881 ggttttataa aacttcccac atttaaaact tcatttgtat atctaaatac atcttttaga
    20941 ttatttacat ctacatcata gttctcaggt tctctagtat ccttatgttg gtctacatgc
    21001 actaacttgc atccttttgt aaactcaccc tttaacaaag actttgccca aaaataaaat
    21061 gcatggttat gattgtcaaa cacatatatc tttttatttc ccaggacata ttcgaccata
    21121 ttctccaatc cttttgcttt tatttcctta ttttcatcta cttcattaaa tactacctca
    21181 ttgcctaacc ttacatcatt taatgtacca tttataagtt ttggaacgta tatgctctta
    21241 ttttctcttt tatcatatga aaatacatta tttcctactg gtttttctat ataaaatcca
    21301 ctatattcat aattttccat aaaacttt
//
LOCUS       DAELLV010000041        21300 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.041, whole genome shotgun sequence.
ACCESSION   DAELLV010000041 DAELLV010000000
VERSION     DAELLV010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21300
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.041"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(173..433)
                     /locus_tag="KPD58_001289"
     CDS             complement(173..433)
                     /locus_tag="KPD58_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich small domain-containing protein"
                     /protein_id="HBG0265071.1"
                     /translation="MGENYKFFNHKDCEFFPCHKTNKPEEFNCLFCYCPLYALGENCG
                     GNFKYTDKGIKDCSSCILPHKKDNYNYIMSKFQDLVKITSKK"
     gene            650..1933
                     /gene="hflX"
                     /locus_tag="KPD58_001290"
     CDS             650..1933
                     /gene="hflX"
                     /locus_tag="KPD58_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBG0265072.1"
                     /translation="MKEIQEKALLVGLNLTTIVKKNDDIDTNESMEELKELTKAAGAE
                     VVGSLIQNKHSVDAAYYIGKGKVEEIRAYSDSLDATLVIFNDELSGAQIRNIENVVGR
                     KVIDRTTLILDIFAQRALSKEGKLQVELAQLKYRLPRLYGMGGEMSRTGAGIGTRGPG
                     EQKLEIDKRHILNKAADIRRELKEVKKNRETQRVKRLKSNIPIVALVGYTNAGKSTLL
                     NELIKTHKDYEQEKEVFVKDMLFATLDVTLRKALLPNKKEFLVVDTVGFVSKLPHDLV
                     EAFKATLEEVQYADLILHVIDATNTSYELQKSTTEGVLKELGVNDKKHILVYNKVDKL
                     ELDIYPKSQEDIVYISAKQGINMDKLLNMIEIALMENTYSVRLMLPYERGDIFSRIKD
                     KYNVENFEYGESGITLDVDLDEEDFNIYREYILEK"
     gene            1941..2618
                     /locus_tag="KPD58_001291"
     CDS             1941..2618
                     /locus_tag="KPD58_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HBG0265073.1"
                     /translation="MDINWENIDNLEDYFITYLLYKESKTVSQISKIRNISSTEASEQ
                     LIQAKLKIKEMQKDDFEASKDILDKFLELDKIKRLDFMDSLDDEKMVYFKRKVFKRIL
                     VEKNAEDLIVLIWATGELKDDRFLKLLHQLTNHRHSDIRRITYSAIRKIESPSSREVL
                     QKGLYDKNAQTRQYCAKALSKLGDENSLKILQQLKDKNKNFEKEYVLRAYDEAIKSLE
                     NAKNLKK"
     gene            2704..3147
                     /locus_tag="KPD58_001292"
     CDS             2704..3147
                     /locus_tag="KPD58_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG0265074.1"
                     /translation="MIVRRCAGGVVFYANKVLIVKNDRGEWTLPKGKILGGGLPYESA
                     VQRVKVETGIDAKMIDVAGDTMYEFFSRSRQQEVCNAIMWYVMEACNTDCVLAPEFQE
                     GGFYKVKDALEMLSHHKEQALVEVSYKKFKELKKLVSEGDDVPAN"
     gene            3452..4096
                     /locus_tag="KPD58_001293"
     CDS             3452..4096
                     /locus_tag="KPD58_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HBG0265075.1"
                     /translation="MSNYRTLHEFGTDEITIEKSVFIGYAKPIKSEEEALEFINEIKK
                     KHKDATHNVWAYTVGKNMNIQRYSDDGEPQGTAGIPTLEVIKKEDLRDVAVVVTRYFG
                     GTKLGAGGLVRAYTKGAKLGLEAGKIIYKVMYQEVKVKIDYTQLGKVQNELMNLGYFI
                     KDTVYEDNVEIVVYSRLEDVEKLSEKMIDITSGTGKIVLGEEFYLSEQNGEILI"
     gene            complement(4182..4559)
                     /locus_tag="KPD58_001294"
     CDS             complement(4182..4559)
                     /locus_tag="KPD58_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBG0265076.1"
                     /translation="MLFALIGFFAGIIGGMGMGGGTILIPALVLLAGIDTKIAQSVNL
                     LSSIPMTLIALAIHIKNKNVIFKLVIPIAVFGVLGAVFGSLLANYLSSDILKKIFGIF
                     LLLVGLFEVKKGFCAKSDKKPLN"
     gene            complement(4559..4945)
                     /locus_tag="KPD58_001295"
     CDS             complement(4559..4945)
                     /locus_tag="KPD58_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBG0265077.1"
                     /translation="MKKTDDLKKKYFDFNLKNTIIGVFTGFINGVFGSGGGTLLVPIL
                     NDIVKVEEHKSHATALSIIIFLTTASSVLYVSKGTYDVNLTIKVAVGSILGGIIGAKL
                     LNKVTGKFLRISFGIIMIIAALRMVF"
     gene            5124..5879
                     /gene="nadE"
                     /locus_tag="KPD58_001296"
     CDS             5124..5879
                     /gene="nadE"
                     /locus_tag="KPD58_001296"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HBG0265078.1"
                     /translation="MSNIKIQIDKTVEWLINKVNEANAKGLIVGVSGGIDSAVVANLI
                     KKAFPENSMGVIMSIKSNPQDREDALKVIEGCDIEYLDLDLIEPQSAILDMVVGNLKD
                     KHLYREEYLKMTDANLRARVRMSTIYTIANNLGYLVVGTDNAAEIHTGYFTKFGDGGV
                     DILPIANLTKGEVYEWAKELGVHEDLINKAPSAGLWEGQTDEDEMGTTYNMIDAVLEG
                     RLDEVPKRDQEIIERLHRLSEHKRKTPAQPPKF"
     gene            5962..6681
                     /locus_tag="KPD58_001297"
     CDS             5962..6681
                     /locus_tag="KPD58_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG0265079.1"
                     /translation="MGRIGNIINRKGKQDAQRAKIFTKHARAIAVAAKEGGADPEYNA
                     ALKTAIEKAKADNMPNDNIDRAIAKGAGAGAGEDYETIVYEGYGPGGVAVIVETLTDN
                     KNRTAGNVRYYFDKNGGNLGTSGCVSFMFDKKGQILVGLGDGVSEEELMDVALEAGAE
                     DFITEEDGYEIITTPEDFSSVRDELKAKGYEFISADVKMIPQTTTVLTEESHLKMMNK
                     LVDMLEEDDDVQDIYHNWEVE"
     gene            complement(6848..7852)
                     /locus_tag="KPD58_001298"
     CDS             complement(6848..7852)
                     /locus_tag="KPD58_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBG0265080.1"
                     /translation="MATIKDIAKDCGLSSATVSYVLSGKGNERRIPLHTQELILNSAD
                     KLGYKRSTPIKHNNIKIAFYWQDEGFELASTNILQAINHIISLEHLNINIIIRPYKVG
                     HLCDDEYFFANNYYDGIVLTGINADDIEYLVKNKPKIPVVVVNREIPGFSSVSIDNEE
                     TGRIAALHAIQNGKNDIAVVFNPTSFTCMTLRGKSFLKTCKEHGIHLSDSIYYCSNKI
                     DSGYDIAQKMLAKNSLKKVIFCNHDVVALGMMNAFHESGIVIGEDIQIFAASNGPSRL
                     MARSIPPLTVIDLKMEETAERSLRMIIDTVTGRINGIQQQVIYPSIIYRQSSPAQNLL
                     "
     gene            8118..9014
                     /locus_tag="KPD58_001299"
     CDS             8118..9014
                     /locus_tag="KPD58_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HBG0265081.1"
                     /translation="MSKVNIFEVGPRDGFQNLKEFLETDKKLKIIDGLIASGIKQLEI
                     SSFVSPKAIPQMKDAKEVATYCVEKYPDVKLYALVPNLYGAKVAWECGIRDISYVISL
                     SETHNKKNINRTHEESIDELKKIMNTYPDMNICIGMATVFGCPFEGLPKIEDAIRFAK
                     CLWENGIREINLADTIGIADPKQVRETISALVEALPECRWQVHIHDTRNMGMVNTLVA
                     IESGITTVQSTLGGLGGCPFAPGASGNTATEDLVYMLERMGYDTGVDFVKLLELSKYQ
                     KSFVNGVYSGHHVNIKSDSCNC"
     gene            9151..10413
                     /locus_tag="KPD58_001300"
     CDS             9151..10413
                     /locus_tag="KPD58_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265082.1"
                     /translation="MDIIALLLFLGVIIIAFVRKNNAGILALAIGVIAVRIFGMDDKE
                     LYGAISNSMFTTLVGITLLFAIVNSTGALKLVAEKIVAASGKRVWLIPIAVFIAGFVV
                     AGVGPGAIPALAIIPAMAVPVAITVGYNPVMLALIGECGLMAGRMTPITPEGQIITTV
                     AEGVGIENVMPTILACQTLSTIVFSIVLFIIFKGYKLKKPINVIDSKQIEKFNIKQII
                     SLISIVVMIAFIIIFDMNVGLAAFAVSAVLIFFNIADEGECIKSIPWSTIVMVLGVGA
                     MMTIVDAAGGIDLMSNALSSLMNSKTATPIMSISAGLMSMVSSALAVVYPTMMPMCVD
                     IAKAVGGVDPLALIAAVGVGGSLAGVSPLSTGGALILAAMGSSKKDFNKEEQNKVFVQ
                     LFIMSAVGLLVIAVVSILSFNAITHLMN"
     gene            10489..11682
                     /locus_tag="KPD58_001301"
     CDS             10489..11682
                     /locus_tag="KPD58_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HBG0265083.1"
                     /translation="MKKGALSNIKVLDLTRVLAGPYCTMMLADFGADVIKIEMPRKGD
                     DTRGFGPFVNGESLYYANINRNKKGITLNLKSEEGKNIFLEMVKHADVVVENYRPGVM
                     EKLGLGYDELAKVNNQIIYGAVSGFGCYGPYSERPGYDIIAQAMGGLMSITGQEGGEP
                     TRVGNAMGDVLGGMNLTIGILTALNARKIIGKGQRVDVSLVDSVVSSLETGIQRYFDS
                     GQPELMGNRYASAYPYDSFKASDGSFVIGCGNDKLFEKLCKKVLFREDLLIDERFKTN
                     LNRCEHYKALKPEIEKWSVKHTIKEAVDLISEAGVPAAPIYNLDCVTTDKHIAKAREM
                     IVDISHPIIGNMKVNGNPVKLMTTKAKIDMPAPSLGESNEEIYNGWLGFDSDKIKDLK
                     ENGVI"
     gene            12311..13105
                     /locus_tag="KPD58_001302"
     CDS             12311..13105
                     /locus_tag="KPD58_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HBG0265084.1"
                     /translation="MESVMENLNNLKVEVKDKVCVITINRPKALNALNSDTLRELSQV
                     IDVVSENEAILGVIITGEGKVFVAGADIRQMQNYKSEEGRKYAGYAQGIFDKIEALEK
                     TVIAAVNGYALGGGCELAMSCDIRIASEKAIFGQPEVNLGVIPCFGGTQRLSRLVGTG
                     IAKELIFTGRQVNAEEAKSIGLINKVVPSDLLLEESMKMMNQIVEKAPIAIRYAKVVI
                     NKGIDMDLKNALELEKDVAGLTFATRDKQEGMNAFIEKRKPVFENK"
     gene            13200..14540
                     /locus_tag="KPD58_001303"
     CDS             13200..14540
                     /locus_tag="KPD58_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBG0265085.1"
                     /translation="MNTGVIISLVGIIIALSLLVVMVMKGINIFIIAIVCSSLVAITG
                     SLNVYDALKVDYMTGFVGFLQANFFIFLTGTLMGKIMEITGGAKSIAKMIVRWIGKDK
                     ALLSIPIACGILAYGGVSVFVVSFAVFPIALEIFKEADLPRRFIPAALTFGCSTFAMV
                     APGAPQIQNIVPASTLGTDIMAGAVNGFISCAVMFIVGSTILYKMVSKEKANGGHFIA
                     HESDIFDEAEATNSKRENGPNGVIALIPLIVSILIINVKVNGKAIVPIEVGVFIGALL
                     VYLLLNKYQDNTKIVGHVGDACKTTVVAICNTCAVVAFGSVVKSAVGFDFIVNAMTNI
                     PGPPIAGAAVGTTVIAGICGSASGGLGIAAPLLGPVFLAKGVSAAALTRTMAISSAAL
                     DSLPHNGYIVTVTNGLCKETHKDAYMPIFWVTVLTPFIGTIVAVLLFTMFPGLP"
     gene            14677..15909
                     /locus_tag="KPD58_001304"
     CDS             14677..15909
                     /locus_tag="KPD58_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HBG0265086.1"
                     /translation="MLNKPLEGVKVIDLTYFVAGPGTSKILADWGADVIKVEPSFGDP
                     GRKTGATMTMPIDDYNNPFYSTYNSNKRGLSINLKSETGIEIMDKLLSEANVFVSSYR
                     TGALKRLGLDYESLSKKHPHLIWGQINGFGDFGPAKDNAGFDTVAFWARSGAMLDIAE
                     KDTSPINPPIAFGDATTSCSLSGGICAALYQQAKTGKGQKVMASLFGQAIWNASSLLA
                     STQFSDEYPKTRKNAISPVINSYRCKDGKWIFLSILEHERYFEALCRLFGREDLIKEE
                     KFASSVESKKNATELINILDGEFAKFTQNEMVEKLTAADIAHEKIQHVNDVVTDEQAI
                     ANNYIYEHTSKDGNKTMLASTPVKFGNIEVNMTCDAPLIGEHSDEILRELGYSDSDIE
                     TLIESGIVTMNKEACEIC"
     gene            15949..17091
                     /locus_tag="KPD58_001305"
     CDS             15949..17091
                     /locus_tag="KPD58_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HBG0265087.1"
                     /translation="MDFRLTEAQLMLQRVAKEFAENEIAPIAAETDKTGIFPRELFSK
                     MAKIGFNGIGTPVEYSGSGGADIEKVIVVTEIAKKCAASAAILSIHTIYAQAILKFGT
                     EEQKKKYLPMMAEGGCVGAFALTEPNAGSDAARAATTAIIDEETDEYVLNGTKCFISG
                     GGQAESLIIFALTDPSKGIKGMSAIIVDKGTPGFSIGKIEEKMGIHGSETAELIFDNC
                     RVPKSNLLGKEGKGFNIAMTCLDGARIGVGAQAVGIAEGALEESIKYSKERVQFGKPI
                     SALQGIQWYIADMATMVESAKLLVYYAADLKARGEKHTKEAAMAKYNASRTAREVTNL
                     ALQIHGGYGYMKDYPLERMYRDAKITEIYEGTSEIHKVVISRAVLG"
     gene            17102..17896
                     /locus_tag="KPD58_001306"
     CDS             17102..17896
                     /locus_tag="KPD58_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBG0265088.1"
                     /translation="MNIVVCLKQVPDTNEVKINKETGTLIRDGVPSIINPDDRNALEE
                     ALKMKDELGAVIKVISMGPPQAKSALKEALAMGADEAYLISDRAFGGSDTWATSTIIA
                     AAIEKVGKYDVIFCGRQAIDGDTAQVGPEVAEFLGIPQVTYAKEVKVQDDKLLVTRYT
                     ETGDYLIEAKMPVLLTAIKELNNPRYPSVKGILEAYNNGDAKITVLTLADLDVDTTQI
                     GLKGSPTNVYKSFVPVKDKHNEIIEGINKKEKAEKLIEILFDLKLV"
     gene            17907..18929
                     /locus_tag="KPD58_001307"
     CDS             17907..18929
                     /locus_tag="KPD58_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBG0265089.1"
                     /translation="MMRAKVNQGINLNDYNGVWVIGEQREGKINPVTIELIGEGRKLA
                     DQLGKELAVVIAGYEVEKEVKELLHYSVDKVYYINDPLLKDFTTDGYAISIANLIERK
                     KPEVVLVGATSIGRDIAPRIAGKVGTGLTADCTKLEIDSTDNKLLQTRPAFGGNLMAT
                     IVCPKNRPQMSTVRPGVMAKAVRNESETGILEVVTPELTEKMIRTRLVEILPQEKKSV
                     NLTDARIIVSGGRGLKRAEGFELIKELADKLGAEIGASRAAVDSGWIEHSHQVGQTGT
                     TVRPELYIACGISGAIQHLAGMSDSKYIVAINKDAKAPIFSICDYGIVGDLYEIIPEM
                     IESLNR"
     gene            19028..20404
                     /locus_tag="KPD58_001308"
     CDS             19028..20404
                     /locus_tag="KPD58_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBG0265090.1"
                     /translation="MKEQWYKDIFARVLSMTDDGFIVVNTSGVIIDINDKYCDFLGKE
                     RKDIIGQNIQSIIPNTKMLDVMKNKYCEEGAIHHYSGGNTKEKSVIVSRSYVENDNGE
                     VVAGVAQVKFRLQSFDVAKKLMSEYMELQYYKEQFKDNCGFDKLIGENRDFIELKKTG
                     VKASKTNFPVLLTGETGTGKEVFARAIHNNSSRSDKPMVSINCAAIPEELLESELFGY
                     DEGAFTGAKKGGKKGKFLVANNGTIFLDEIGDMPLTMQAKLLRVLQESEIEPVGGLKT
                     IKIDVRVISATRKNLSKMVEEGLFREDLYYRLNVINIHMMELKDRQDDILLLANYILN
                     KLNVEYKELKVLSDKVKNCFINYTWPGNIRELQNVIKSAYAVSDDMVIMMCDLPSKMD
                     NISRVAQCNVDSNCSIHEMVENYEKSLIIDVLRKYNWKCSKAAEVMGIHKSLLYKKIK
                     KYEIELNN"
     gene            complement(20736..21080)
                     /locus_tag="KPD58_001309"
     CDS             complement(20736..21080)
                     /locus_tag="KPD58_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265091.1"
                     /translation="MDQIKRLHELQQKSYLTKEEFEELKNNTYIERFELRDECDNSYI
                     YTFYTNDKCNDIKFIKSQFIVTLIKIGFLCECGGVFRQKKIVDEYDYGYNAIYKCDSC
                     GKELDKEVENYD"
BASE COUNT         7995 a         2520 c         4099 g         6686 t
ORIGIN      
        1 atctttatca ataaaaatat ttcaatgtta aatagaggga tatttttatt tccaaaataa
       61 gataataaaa aaggattaga ttaaaagtta tacaataaaa atagctgtgc aataatattt
      121 agactatgaa agtagttgaa aaatatttat gcacagctat ttataataga atttactttt
      181 tactagtgat tttaactaaa tcttgaaatt tactcataat gtaattgtaa ttatcttttt
      241 tatgaggtaa tatacaacta ctgcaatctt tgataccttt atctgtatac ttaaagtttc
      301 caccacagtt ttctcctaaa gcatacaaag gacaatagca aaatagacag ttaaactctt
      361 ctggtttatt agttttatga caagggaaaa attcgcaatc tttgtgatta aaaaacttat
      421 aattttcgcc catattttcc tcctaattaa ataaaattta aattttttat actatataag
      481 attttggtga taatataatt atactaaata attttatgaa tagtcgataa tatagtatta
      541 ttaatcatag tattaatggt ataattagtc aacagaaaaa taatgtaaaa ataatgtaag
      601 tgtattttgt aattagaata tgttttataa caaaaaatga ggtgattaag tgaaagaaat
      661 tcaagaaaaa gcacttcttg taggtctgaa tttgactaca atagtgaaaa aaaatgatga
      721 tatagacact aatgaatcta tggaagaatt aaaagaacta acaaaagctg ctggggcaga
      781 agttgtaggt agcttaattc aaaataaaca ctctgtagat gcagcttatt atataggaaa
      841 aggtaaagta gaagaaataa gagcctatag tgactcttta gatgctactt tagtaatttt
      901 taatgatgaa ttatctggtg ctcaaatcag aaatatagag aatgttgttg gaagaaaagt
      961 aatagataga actactttaa ttttagatat atttgctcaa agggcactta gcaaagaagg
     1021 taaattacaa gttgaattag cacaattaaa gtatagattg ccacgacttt atggaatggg
     1081 tggtgagatg agtagaacag gtgcagggat tggtactaga ggtcctggag aacaaaaact
     1141 agaaatagat aagagacata tacttaataa agcagcagat ataagacgtg aacttaaaga
     1201 agtaaaaaag aatagagaga cacagagagt taagcgttta aagtcaaata taccgattgt
     1261 agcacttgtt gggtatacaa atgcaggaaa atctaccctg ttgaacgaat taataaagac
     1321 acacaaggat tatgaacaag aaaaagaagt ttttgtaaaa gatatgcttt ttgcaacttt
     1381 agatgtaact ttgagaaagg ctcttttgcc gaataaaaaa gaatttttag tagtagatac
     1441 tgttggattt gtaagtaaac ttccccatga tttagtagaa gcgtttaagg caacattgga
     1501 agaagtacag tatgcagatt taatcttaca tgtaattgat gcaacaaata ctagttatga
     1561 attgcaaaaa agtacgactg aaggtgtatt aaaagaactt ggtgtaaatg ataaaaaaca
     1621 tatacttgta tataataaag tggacaagct agaattggat atatatccta aaagtcaaga
     1681 agatattgtg tatatttctg caaaacaagg cataaatatg gataagttat taaatatgat
     1741 agaaattgct ttaatggaaa atacctattc agtaaggttg atgttacctt atgagagagg
     1801 tgatatattt agtagaataa aagataaata taatgtggag aattttgagt atggggaaag
     1861 tggtataacg ctagatgtag atttagatga agaagatttt aatatctata gagagtacat
     1921 actagaaaaa taggttgaat atggatataa actgggaaaa tatagataat cttgaagatt
     1981 attttataac ctatcttctt tataaagaat ctaaaacagt atctcaaatt agtaaaatta
     2041 gaaatatttc aagtactgaa gcaagtgagc aacttataca agcaaagtta aaaataaagg
     2101 aaatgcaaaa agatgatttt gaggcatcta aggatatttt ggataaattt ctagaactag
     2161 ataaaattaa gagacttgat tttatggata gcttagatga cgaaaaaatg gtatacttta
     2221 agagaaaggt tttcaaaaga atccttgtag aaaaaaatgc tgaagattta attgttttaa
     2281 tttgggcgac aggtgagcta aaagatgata ggtttttgaa attgcttcat cagctcacaa
     2341 accacagaca ctcagatatt agaagaataa cttattcagc aatcagaaag attgaatcac
     2401 caagtagtag agaagtactg caaaaaggtc tctacgacaa aaatgcacag actaggcagt
     2461 actgtgcaaa agctctttca aaattgggag atgaaaacag tttaaagatt ttacagcagt
     2521 tgaaagacaa gaataagaat tttgaaaaag aatatgtgct tagagcttat gacgaagcca
     2581 ttaagtcatt ggaaaatgca aaaaatttga aaaagtaatt ttttattaaa gcaatttgtt
     2641 tacttaatga tgtatttcta aagtttaaca aggtaataaa taaattaatg ggaggggttt
     2701 caaatgatag taagacgttg tgcaggtgga gtagtgtttt atgctaataa agtacttatt
     2761 gtaaaaaatg ataggggtga atggacactt ccaaaaggaa agatactagg agggggactt
     2821 ccatatgaga gtgctgttca aagagttaaa gtagaaactg gcatagatgc taaaatgata
     2881 gatgtggctg gcgatactat gtatgaattt ttctcaagaa gtagacaaca agaagtttgt
     2941 aatgcaataa tgtggtatgt tatggaagct tgtaacactg attgtgtact tgctcctgaa
     3001 tttcaagaag gtggatttta taaagtaaag gatgctttag aaatgttatc acatcataaa
     3061 gagcaagctt tagtagaagt atcttataaa aagtttaagg aattaaagaa attagtttca
     3121 gagggcgatg atgttccagc aaattaattg tgaatttaga ggattctgag gaagggatag
     3181 ccccttcctt ttttaattga agcagtttta agcgtaaatt atatagtctc tttaaactat
     3241 gatttatcaa tttagagatt tagtagttta tatttaaaat cagtaagata tgtttaaaat
     3301 aaaaattaaa aagtatacaa ttttattata taattgtctt atgatgttga ttaaagaatt
     3361 attaaaagtc catttatttt taaatagaat aattttataa atatatattt gatgtgtata
     3421 ttagaaaatg attaaaatag gaggaaatac aatgagcaat tacaggacat tacatgaatt
     3481 tggaacggat gaaataacga ttgaaaagtc tgtttttatt ggatatgcta agcctataaa
     3541 gagtgaagaa gaagcattag agtttataaa tgaaataaag aaaaagcata aagatgcaac
     3601 tcacaatgtg tgggcttata ctgtaggtaa aaatatgaat atacaaagat atagtgatga
     3661 tggagaacca caaggaacag ctgggatacc tacattagag gttataaaaa aagaagattt
     3721 gagagatgta gcggttgttg ttactaggta ttttggagga acaaaattag gtgcaggtgg
     3781 tctagttaga gcttatacaa aaggtgctaa actgggactt gaagcaggta agataattta
     3841 taaggtaatg tatcaagaag ttaaggttaa gatagattat actcaacttg gaaaagtgca
     3901 aaatgagtta atgaatttgg ggtattttat aaaagatact gtatatgagg ataatgttga
     3961 aatagtagta tattcaagat tagaagatgt agagaaatta tcagaaaaaa tgattgatat
     4021 aacaagtgga acaggaaaaa tagttttagg agaggaattt tatttgtctg aacaaaatgg
     4081 agaaatttta atataatcta aaaggttatt agaatagtta atacctcaaa tagccaatta
     4141 gtatttttaa gtgaaaagcc attaaattga tttaatctaa atcaatttaa tggcttttta
     4201 tcggattttg cacagaatcc tttttttact tcaaataaac cgactaaaag taaaaatatc
     4261 ccaaatattt tttttaatat atctgaagac agataattag ctaatagtga accaaataca
     4321 gctcctaaaa ccccaaacac agctataggt ataaccaatt taaaaattac atttttattt
     4381 tttatatgta tagcaagtgc tataagtgtc atagggatag atgaaagtaa atttacgctt
     4441 tgtgctattt tagtgtctat gccagcaagt aaaacgagtg caggtataag gattgttcct
     4501 ccacccattc ccattcctcc aataattcct gcaaaaaatc caattagagc aaataacatt
     4561 aaaacaccat ccttaatgct gcaattatca taattattcc aaaagaaatt ctaagaaact
     4621 ttccagtaac cttatttagt aatttagctc ctataatacc accaagaata cttccaactg
     4681 caactttaat tgttaagtta acatcataag tacctttaga tacatataaa acagaacttg
     4741 ctgtcgttaa gaatattatt atagaaagag cagttgcatg agatttatgt tcttctacct
     4801 taacgatgtc atttaatatt ggaactaaaa gagtacctcc tccagaacca aaaactccat
     4861 ttataaatcc tgtaaaaact ccaattatcg tattttttaa gttaaaatca aagtattttt
     4921 tctttaaatc atcagttttt ttcataaaaa tcaccttctg aaatagtttt accaaaatag
     4981 gcaaattata tttatataaa ataagattaa atataatctt tcatatttgt ttaattttgc
     5041 ataaaaaaaa acatattaaa acaacatatt aatatatggt ataatatatt aaaacaaagt
     5101 agcaaatttg agaggagata acaatgtcaa atataaaaat acaaatagac aaaactgtag
     5161 aatggcttat aaataaggtt aatgaggcaa atgcaaaagg attaattgtt ggtgtatctg
     5221 gaggaattga ttcggcagta gttgctaatt taattaaaaa ggcatttcca gaaaactcta
     5281 tgggagttat aatgagcata aaaagtaatc ctcaagatag agaagatgca cttaaggtta
     5341 tagagggatg tgatatagaa taccttgatt tagaccttat agaaccacaa agtgctatat
     5401 tagacatggt agtaggaaat ttaaaagata aacatttata tagagaagaa tatttaaaga
     5461 tgacagatgc aaacttaaga gcaagagtaa gaatgagtac aatttataca attgctaaca
     5521 atctaggata tctggttgtt ggtacagaca atgctgcaga aatacacaca ggatatttta
     5581 ctaaatttgg tgatggtgga gttgatattt tacctattgc aaatttaact aaaggagagg
     5641 tgtatgaatg ggcaaaagag cttggtgttc atgaagattt aataaacaaa gctccttcag
     5701 caggtctttg ggaaggtcaa acagatgaag atgaaatggg aactacttac aatatgatag
     5761 atgctgtgtt agaaggtaga ttggatgaag ttcctaaaag agaccaagag ataattgaaa
     5821 gacttcatag attaagtgaa cacaagagaa aaacgcctgc acaacctcct aagttctaat
     5881 ttactattta taagatttgt gataaaattt acaagtgatg aaaattagaa taattaattg
     5941 tacgtattaa ggagtggaaa tatgggacgt ataggtaata taattaatag aaaaggtaaa
     6001 caagatgctc aaagagcaaa gatatttact aaacatgcta gagcaatagc agttgcagct
     6061 aaagaaggtg gagcagatcc agagtacaat gcagcgttaa aaacagctat agaaaaagct
     6121 aaggcagata atatgccaaa tgataatata gatagagcta tcgctaaagg tgctggagca
     6181 ggtgctggag aagactatga aactatagtt tatgaaggat atggacctgg tggagttgct
     6241 gttatagtag aaactttaac tgacaacaaa aacagaactg ctggtaacgt aagatattat
     6301 tttgacaaaa acggaggaaa cttaggaact agtggatgtg tgtcttttat gtttgataaa
     6361 aaaggacaaa tattagttgg tttaggagat ggagtttcag aagaagaatt gatggatgtt
     6421 gcattagaag caggagctga agatttcata acagaagaag atggttatga aataataact
     6481 acaccagaag atttttctag tgttcgtgat gagttaaaag ctaaaggata tgagtttata
     6541 tctgctgatg tgaagatgat acctcaaact acaactgtat tgactgaaga atctcattta
     6601 aaaatgatga ataaattagt tgatatgctt gaagaagatg atgatgttca agatatatat
     6661 cataactggg aagtagaata aatagtataa gcatattgaa atttataagt attgaatcaa
     6721 ttttaacatg tgataaattt tatagaataa gaaacactaa aatagacata cgcacctcta
     6781 acctatattt gcataataat atgtaagcat aggtaagggg tgtttttata tttataggtt
     6841 aagaaattta aagtaaattt tgagctggac tcgattgtct ataaataatt gatgggtata
     6901 taacttgttg ctgaatacca tttatacgcc cagttactgt atcaataatc atcctaagac
     6961 tacgttctgc tgtctcttcc atttttaaat ctataactgt caaaggagga atacttcttg
     7021 ccattagtcg tgaaggtcca ttacttgctg caaatatttg aatatcctct ccaattacaa
     7081 ttcccgattc atgaaatgca ttcatcatac caagagctac cacatcatga ttacaaaaaa
     7141 ttactttttt tagtgaattt tttgcaagca ttttttgagc aatatcatat ccactatcta
     7201 ttttattaga acaataatat atagaatctg atagatgtat tccatgttct ttacatgttt
     7261 ttaaaaaaga tttacccctt aaagtcatac atgtaaagga agttgggttg aatacaacag
     7321 caatatcatt tttaccattt tgaatagcat gtaaagctgc tatacgacca gtttcctcat
     7381 tatcaataga tacacttgaa aaaccaggta tttcacgatt tacaacaact acaggaattt
     7441 taggtttatt ttttactaga tattctatat catcagcatt gatacctgtt aaaacaattc
     7501 catcataata attatttgca aaaaagtatt catcatcaca tagatgacca actttgtaag
     7561 gtcgtatgat gatatttatg tttaaatgtt ctaaagaaat tatgtggttt attgcttgaa
     7621 gtatattggt tgatgccaac tcaaatccct catcctgcca ataaaaagct attttaatat
     7681 tattatgttt aataggagta gagcgtttat atccaagctt atcagctgaa tttaatataa
     7741 gctcttgtgt atgtagcgga atcctgcgtt catttccttt accacttaag acgtacgaga
     7801 cagttgctga agaaagacca cagtcttttg caatatcttt gattgttgcc atgataaaat
     7861 cctccttaaa tagttgctta aatttgctta taccaataat cttaacatat attcaactaa
     7921 agaacaatat tctacaagtt gcatatttta caggattaga ataaataaat ttaaaaaaac
     7981 ttcttactta tgttgacata atttagttta taggattata atatgcttaa ataagcttat
     8041 aattaactta aattaagcaa agttaagcag ggacatgcaa tgctagcttg caatgtgact
     8101 gtaaaggggg aaaaggtatg agtaaggtaa atatatttga agttggtcct agagatggat
     8161 ttcaaaacct gaaagagttt ttagaaactg ataaaaagct taagattatt gatggtctta
     8221 ttgcttcagg gattaaacaa cttgaaatta gttcatttgt aagcccaaaa gcaatacctc
     8281 agatgaaaga tgcaaaagaa gtagcaactt attgtgtaga aaaatatcct gatgtgaaac
     8341 tttatgcatt agttccaaat ttatacggtg ctaaagttgc atgggagtgt ggtataagag
     8401 atatatccta tgttatatct ttgagtgaaa ctcataataa aaaaaatata aatcgtaccc
     8461 atgaagagtc tattgatgag cttaaaaaaa taatgaatac ttatccagac atgaatatat
     8521 gtattggaat ggctactgta tttggatgtc cttttgaagg tttaccaaaa attgaagatg
     8581 ccataagatt tgctaaatgc ctttgggaaa acggtatcag ggagataaat ctagcagata
     8641 caatagggat tgctgaccct aagcaagtaa gagaaactat tagtgcatta gttgaagctt
     8701 taccagagtg cagatggcaa gtacatattc atgatactag aaatatggga atggttaata
     8761 ctcttgtggc tattgaatct ggaattacta ctgttcagtc tacacttgga ggattaggag
     8821 gatgcccatt tgctcctgga gcatctggca atactgcaac tgaagattta gtatatatgc
     8881 ttgaacgtat ggggtatgat acaggagttg attttgttaa acttcttgaa ttatcaaagt
     8941 atcagaagag ttttgtaaat ggagtatata gtggtcatca tgtaaatata aagtctgata
     9001 gttgtaattg ttagttctat tgattaatcg attaaaaaat atgttgatta aaaaaatatg
     9061 ctgattggta aaatagattt attgataaaa aatataaatt ataaaataca ttatctaata
     9121 aaacaaataa aaaaattgag ggggattagt atggatatta tagctttact tttattttta
     9181 ggagttatta ttattgcatt tgtacgtaag aataatgcag gtattttggc tctagctatt
     9241 ggtgtcatag ctgttagaat ttttggaatg gatgataaag aactttatgg agctataagc
     9301 aactcaatgt ttactacact tgttggtata acactactct ttgcaatagt gaactcaaca
     9361 ggagcattaa agcttgttgc agaaaaaatt gtagctgctt ctggtaaacg tgtttggctt
     9421 atacctatag cagtttttat agcaggattt gttgttgctg gtgttggacc tggggcaatt
     9481 ccagcacttg caattatccc agcaatggct gttcctgtag ccataacagt tggttataac
     9541 cctgtaatgc ttgcacttat aggtgaatgt ggattaatgg caggtcgtat gactcctatt
     9601 actccagaag gtcaaattat aacaaccgtt gcagaagggg ttggaattga aaatgttatg
     9661 cctactatct tagcctgtca aactctttct acaattgtat tttcaatagt attgtttata
     9721 attttcaaag gatataaact taaaaaacct ataaatgtta tagatagtaa gcaaatagaa
     9781 aaattcaata taaagcagat aatttctcta ataagcatag tagttatgat tgcttttatc
     9841 ataatctttg atatgaatgt tgggcttgca gcttttgcag tttcagctgt acttattttt
     9901 ttcaatattg ctgatgaagg agaatgtatc aaatcaattc catggagcac gatagtaatg
     9961 gttcttggag taggtgcaat gatgacaatc gtagatgcag caggaggtat tgacttgatg
    10021 agtaatgcat tatcatcact gatgaatagt aaaacagcta ctccaattat gagtatttca
    10081 gcaggactta tgtctatggt tagttctgct ttagcagtag tttatccaac tatgatgcct
    10141 atgtgtgttg atatagctaa ggcagttgga ggagtagacc cactagcact tattgctgca
    10201 gttggtgttg gaggttctct tgctggtgta agcccacttt ctactggtgg ggcattgatt
    10261 ttagcagcta tgggttcaag taagaaagac tttaataagg aggaacaaaa taaggttttt
    10321 gtacagcttt tcattatgag tgcagttggc ttattggtta ttgcagtagt atcaatatta
    10381 tcatttaatg caataacaca tttaatgaac taattagaat aactgaaaat aaatcattaa
    10441 atttaacttt taatattaat caatataata taatttggag gggaagttat gaaaaaagga
    10501 gcattaagca atataaaggt acttgacctt acaagagttc ttgcaggtcc atattgtaca
    10561 atgatgttgg cggattttgg agctgatgtt ataaaaatag agatgccaag aaaaggtgat
    10621 gatacaagag gttttggacc ttttgtaaat ggagagagtt tatactatgc aaatataaat
    10681 agaaataaaa aaggtatcac tctaaacctt aaatcagaag aaggaaaaaa tatattttta
    10741 gaaatggtaa aacatgctga tgttgtagta gaaaattaca gaccaggtgt aatggaaaag
    10801 ttaggtctgg gttatgatga gttggctaaa gttaacaatc aaataattta tggagctgta
    10861 tcaggatttg gatgttatgg accatattca gaacgtccag gttatgatat tattgcacag
    10921 gcaatgggtg gtcttatgag tataacaggg caagaaggag gagaaccaac gagagttggt
    10981 aatgctatgg gtgatgtact aggtggaatg aatttaacta ttggtatact cacagcactc
    11041 aacgctagaa aaataatagg aaaaggacaa agagtagatg tttccctcgt tgattctgtt
    11101 gtatcgtctc tcgaaactgg tatacagaga tactttgatt caggacaacc tgaacttatg
    11161 ggtaatagat atgcttcagc ctatccttat gactctttta aggcaagtga tggaagtttt
    11221 gtaataggct gtggtaatga caaacttttt gaaaagttat gtaaaaaagt tctttttaga
    11281 gaagaccttt taatagatga gagatttaaa acaaatctta atcgttgtga acattacaag
    11341 gctcttaaac cagaaataga aaaatggtct gttaagcata caataaaaga agcagttgac
    11401 ttaatatcag aggctggagt tcctgctgct cctatctata atcttgattg tgttacaaca
    11461 gataaacata tagcaaaagc aagagaaatg attgtagata ttagtcatcc tattatagga
    11521 aatatgaaag tcaatggaaa tccagttaag ttgatgacaa caaaagctaa aattgatatg
    11581 ccagcaccat ctctagggga aagtaacgag gagatttata atggttggct tggttttgat
    11641 agtgataaaa taaaagattt aaaagaaaat ggagttattt gattaaaaaa tagctaaaca
    11701 cacttgtgac tttagtcatg ggaggttcag ttagaataat atcaaaaaat attaaatcaa
    11761 tactgtaaat caaatataga attgtcagaa gcatttaaga tgggaaaagc tacttagaca
    11821 ttgagtcttt gtggcttttc ctattttcat taagaatttt atattatcag aatagatatg
    11881 tactaaacta aagtattaac aatttaaatt aatagaaaat aaactaccta attatgttga
    11941 ataaagacta ctttagtgaa aacaaactta tagagatgct atttaaatta ttttaatgga
    12001 aatatcaaaa atgagactgc attctcaaaa atgagactgc gttttttaag tattttctcg
    12061 aaaatgagac aaaaatattg ttttatgggt tttattgaag aaaaattctt ctgtgaatta
    12121 caatttaatc aattttaaag ttgaaaatta ttaaaaattt agtgaaattt atgtatattt
    12181 cgtttatttt attaaaaata acaatttttt actaaaaata aagaacttca atggtgattg
    12241 gcatagtact tgctatttta tattaacaag aaatgtagaa aaaataacaa tgtataatgg
    12301 ggggaatatg atggaatctg taatggaaaa tttgaacaat cttaaggtgg aggttaagga
    12361 caaagtctgt gttattacta taaatagacc taaagcttta aatgctctta atagtgacac
    12421 tcttagggaa ctatcacaag ttattgatgt agtatcagaa aatgaggcaa tattaggagt
    12481 tattattact ggagaaggta aagtttttgt agccggtgct gatatacgcc agatgcaaaa
    12541 ctataaaagt gaggaaggca gaaaatatgc aggttatgct cagggaattt ttgataaaat
    12601 tgaagctctt gagaaaacag taatagcagc agttaatgga tatgcattag gtggtggatg
    12661 tgaacttgct atgagttgtg atattagaat tgcatcagaa aaggctatat ttggacaacc
    12721 tgaagttaat ttaggagtta ttccatgttt tggtggaact caaagattat caagacttgt
    12781 tggaactgga atagctaaag agttaatttt tacggggaga caagtaaatg cagaagaagc
    12841 taaatctatt ggacttatta ataaagtggt tccaagtgat ttacttttag aagagtctat
    12901 gaagatgatg aaccaaatag ttgaaaaagc gccaatagca ataagatatg caaaagttgt
    12961 tattaacaaa gggatagata tggatttaaa gaatgctctt gaactagaga aagatgttgc
    13021 aggtttaact tttgctacaa gagataagca agaaggaatg aatgcattta tagaaaagag
    13081 aaagccagtt tttgaaaata aatagctatt ataactaaaa ttggaacaat gtagaatata
    13141 ttcaaaatct aaatttcgtg aaaaaataca tatagtttaa ttttaaaagg agttaattta
    13201 tgaatacggg agtaattata agtttagtag ggattatcat agctctatct cttctagttg
    13261 taatggttat gaagggaata aatattttta ttatagcaat tgtatgttca tcattagttg
    13321 ctataacagg aagcctaaat gtgtatgatg cacttaaagt ggattatatg acagggtttg
    13381 ttggatttct tcaggcaaat ttctttattt tcctaacagg gacattaatg ggaaaaataa
    13441 tggagattac tggtggagca aaatctattg ctaagatgat tgtaagatgg attgggaagg
    13501 ataaagcact actttctatt ccaatagctt gtggaatatt agcctatggt ggagtaagtg
    13561 ttttcgttgt aagttttgca gtgtttccga tagctttaga aatttttaaa gaagctgatt
    13621 tgccacgaag atttatacca gcagctttga cttttggatg ctcaactttt gctatggttg
    13681 caccaggggc accacaaatt cagaatattg ttccagcatc aacattaggg acagatatta
    13741 tggctggagc agtaaatggt tttatatcat gtgcagtaat gtttatagtt ggaagtacaa
    13801 ttttgtacaa aatggtttca aaagaaaaag ccaatggtgg tcattttata gcacatgaat
    13861 cagatatatt tgatgaagca gaagcaacta attctaagag agaaaatggt cccaatggtg
    13921 taattgcctt aattccattg atagtgtcga ttttaataat taatgtaaaa gttaatggaa
    13981 aagcgatagt accaattgag gtgggagtat ttattggagc tttattagta tatttattgt
    14041 taaataaata tcaagataat actaaaatag ttggacatgt tggtgatgca tgtaaaacta
    14101 cagtagtagc aatatgtaat acttgtgctg tagttgcatt tggttcagta gtaaaatcag
    14161 cagttggttt tgacttcata gttaatgcta tgactaatat tcctggtcct cctattgcag
    14221 gggctgcagt tggaactaca gttattgcag gtatttgtgg gtcagcttca ggtggactag
    14281 gaattgcagc tccacttctt ggaccagtat ttttagccaa gggtgtttcc gctgcagcat
    14341 taactaggac tatggcaatt tcatcagcag cattagattc acttccacac aatggatata
    14401 ttgttactgt aacaaatggt ctttgtaaag aaacacataa ggatgcctat atgccaatat
    14461 tctgggtaac tgttttaacc ccatttattg gaaccatagt agcagtccta ctgtttacaa
    14521 tgttcccggg attgccatag gagaaaagat aaaaggtata aattatattt tgatattata
    14581 aaatcaattt tcaaaatata tacaataaat ttcatgtttt aatactgtaa atatgtattt
    14641 aaaataacaa attgaccata taaggaggaa ttgaaagtgt taaataaacc attagaggga
    14701 gttaaggtta ttgacctaac gtattttgta gcaggtccag ggacttctaa aatattagct
    14761 gactggggtg ctgacgttat taaggtagag ccaagctttg gagacccagg aagaaagaca
    14821 ggggcaacaa tgacaatgcc aatagatgat tataataatc ctttttacag cacatataat
    14881 tcaaataaaa gaggtttgtc tataaatctt aagagtgaaa ctggaattga aattatggat
    14941 aagcttttaa gcgaagcaaa cgtatttgtt tcaagttata gaacaggagc attaaaaaga
    15001 ctaggtcttg attatgaatc attaagtaaa aagcatccac accttatctg gggtcagata
    15061 aatggttttg gcgattttgg acctgctaag gataatgcag gatttgatac agttgccttt
    15121 tgggcaagaa gtggggctat gcttgatata gcagaaaaag atacaagtcc aataaatcca
    15181 cctatagcct ttggagatgc aacaacttct tgtagtttat ctggaggaat atgtgcagct
    15241 ttgtaccagc aagctaagac tggaaaagga caaaaagtaa tggcatcttt atttgggcaa
    15301 gcaatatgga atgcatcatc tttattagca tctacacagt ttagtgatga gtatccaaaa
    15361 actagaaaaa atgctatttc accagtaata aattcttatc gttgtaagga tggaaagtgg
    15421 atattcttaa gtattttaga gcatgaaaga tattttgagg cactttgtag attatttgga
    15481 agagaagact taataaaaga agagaaattt gcatcttcag tagaaagtaa gaaaaatgct
    15541 acagaactta ttaatatttt agatggtgaa tttgcaaagt ttactcaaaa tgagatggtt
    15601 gaaaaactta ctgctgctga tatagctcat gaaaaaattc aacatgttaa tgatgtagtg
    15661 actgatgaac aggctattgc taataactat atatatgaac atacaagtaa agatggaaat
    15721 aagacaatgc ttgcatctac tccagttaaa tttggaaata ttgaagttaa catgacttgt
    15781 gatgctccac ttataggaga acattcagat gagatattga gagaacttgg atacagtgat
    15841 tctgatatag agactctaat tgaatctgga attgtaacaa tgaataaaga agcatgtgaa
    15901 atatgttaat aaacatgtga agtatgttaa taaataggag ggaattttat ggattttaga
    15961 ttaacagaag cacaattaat gcttcaaaga gtagcaaaag aatttgcaga gaatgaaata
    16021 gcaccaatag ctgctgaaac agataaaaca ggtatttttc caagggagct ttttagcaaa
    16081 atggctaaaa taggatttaa tggaattgga acacctgtag agtatagtgg ttctggtggg
    16141 gcagatatag aaaaggtaat tgttgttact gagattgcca aaaaatgtgc agcatctgca
    16201 gcaatattat caatacacac aatatatgct caagctattt tgaaatttgg tactgaggaa
    16261 caaaagaaaa aataccttcc aatgatggct gaaggtgggt gtgtaggtgc ttttgcctta
    16321 accgaaccta atgctggttc agatgctgcg agagctgcaa caactgcaat tattgatgaa
    16381 gaaactgatg aatatgtttt aaatggaact aagtgtttta tttctggtgg aggtcaagca
    16441 gaatcattaa taatatttgc attgacagac ccatcaaagg gaataaaggg aatgtctgct
    16501 atcattgtcg acaaaggtac tccaggtttt tcaataggaa aaattgaaga aaaaatgggt
    16561 attcatggtt cagaaactgc agagttaata tttgataact gtagagttcc aaagtctaac
    16621 ttattaggaa aagaaggtaa aggatttaat atagctatga cttgtttaga tggagcaaga
    16681 attggagttg gagctcaagc tgtaggaatt gctgaagggg ctttagaaga aagtatcaaa
    16741 tattctaaag aaagagtcca gtttggcaag ccaatttcag ctttgcaagg tattcaatgg
    16801 tatatagcag atatggcgac aatggtggaa tcagcaaaat tattagtata ttatgctgca
    16861 gatttgaaag ctagaggaga aaaacacact aaagaagctg ctatggcaaa atacaatgcg
    16921 tcacgtactg caagagaggt tacaaattta gcccttcaaa tccatggtgg atatggatat
    16981 atgaaggatt atccattaga gagaatgtac agagatgcca aaataacaga aatttacgaa
    17041 ggtacttcag aaatacacaa agttgttatt tcaagagcag tactaggata ggaggaaaag
    17101 aatgaatata gttgtttgct taaagcaagt tccagatact aatgaagtta aaataaataa
    17161 agagacaggt actctaataa gagatggtgt accaagtata atcaatccag atgatagaaa
    17221 tgctttagaa gaagctctaa aaatgaaaga tgaactagga gcagtgatta aggttataag
    17281 catgggacct cctcaagcta aaagtgcttt aaaagaagct ttggcaatgg gagctgatga
    17341 agcttattta atctcagata gagcttttgg tggttcagat acttgggcta catctacaat
    17401 tattgctgct gctatagaga aggttggaaa gtatgatgta atattctgcg gaagacaagc
    17461 tatagatgga gatacagctc aggttggacc agaggttgca gaatttttag ggataccaca
    17521 agttacttat gcgaaagaag taaaggttca agatgacaaa ttactagtaa ctcgttatac
    17581 agagacaggg gattatttaa tagaagcaaa gatgccagtt ttattaactg ctataaaaga
    17641 gttaaacaat ccaagatatc caagtgtcaa gggaatctta gaagcttata ataatggaga
    17701 tgcaaagatt actgtattaa cacttgcaga tttagatgtt gatacaactc aaattggatt
    17761 aaagggttca cctacaaacg tttataagtc ttttgtacca gttaaagaca agcataatga
    17821 aataatagaa ggtataaata aaaaagaaaa agctgagaaa ctaatagaaa tactattcga
    17881 tttgaaacta gtataggagt gttaagatga tgagagcaaa agttaatcaa ggtataaatt
    17941 taaatgatta taatggtgtt tgggtaatag gagagcaaag ggaaggtaaa attaatcctg
    18001 ttacgataga acttattggt gaaggaagaa agcttgctga ccaattagga aaagagcttg
    18061 cagttgtgat agctggatat gaagtagaga aagaagtgaa ggaattactt cactatagtg
    18121 ttgataaagt atattacatc aatgaccctc ttcttaaaga tttcactact gatggatatg
    18181 caatttcaat tgcaaattta atcgaaagaa aaaagccaga ggttgtattg gttggtgcta
    18241 cttctatagg aagagatata gcgcctagaa ttgcaggtaa ggttggaaca ggtctaactg
    18301 ctgactgtac aaaacttgaa atagattcaa cagataataa gttattacaa acaagaccag
    18361 catttggtgg aaatttaatg gccactattg tttgtccaaa aaatagacca cagatgtcaa
    18421 cagttcgtcc aggagttatg gcaaaagctg taagaaacga atcagagact ggaattttag
    18481 aagttgttac tccagaactt actgaaaaaa tgattagaac tagattagta gagatacttc
    18541 cacaagaaaa gaaatctgta aatctaactg atgctagaat aatagtatca gggggaagag
    18601 gattaaaaag agcagaagga tttgagctta taaaagaatt ggctgataaa ttgggggcag
    18661 agattggtgc ttcaagagca gcagtagatt cagggtggat agaacattct catcaagtgg
    18721 gacaaacagg aactactgtt agaccagaac tatacattgc atgtggaata tctggagcaa
    18781 tacaacattt agcaggtatg agtgattcta aatatatagt tgctataaat aaagatgcaa
    18841 aagcccctat ttttagtata tgtgactatg gaatagtagg agatttatat gaaattatac
    18901 ctgaaatgat agaatcatta aataggtaac ttttagaaaa cattgaatat gtagaatgat
    18961 aagtcttata atattgtatt gtaagactta ttatttttgc tgaaggttat agtggggaga
    19021 tgaaacaatg aaagagcagt ggtataagga tatatttgca agagtattat ctatgactga
    19081 tgatggtttt attgttgtaa atacaagtgg agtaataata gatattaatg ataaatattg
    19141 tgacttttta ggaaaagaaa gaaaagatat aataggacaa aatatacaaa gtattatacc
    19201 aaatacaaaa atgctagatg ttatgaaaaa taagtattgt gaagaaggag ctatacatca
    19261 ttattcaggt ggaaatacaa aagaaaaaag tgtaatagta agtaggtctt atgtggaaaa
    19321 tgacaatgga gaagtagttg caggagtagc tcaagttaaa tttagattgc aatcatttga
    19381 tgtagctaaa aagttaatgt cagaatatat ggagctccaa tattataaag agcagtttaa
    19441 ggataactgt ggttttgaca agctcatagg tgaaaataga gacttcatag aacttaagaa
    19501 aacaggtgtg aaagcttcta aaacaaactt tccagtactt ttaacgggtg aaacaggcac
    19561 aggtaaagaa gtttttgcta gagcaattca caataatagc agtaggtcag ataaacctat
    19621 ggttagtata aattgcgctg caataccaga agaattatta gagtcagagc tatttggcta
    19681 tgacgaagga gcttttacag gagcaaaaaa gggtggtaaa aaaggtaaat ttttagttgc
    19741 gaataatggt acaatctttt tagacgagat aggtgacatg cctttgacca tgcaagcaaa
    19801 acttttaaga gttcttcagg aaagtgagat agaaccagtg ggaggtctta aaactataaa
    19861 gatagatgta agagtaatct ctgccacaag aaagaattta agtaaaatgg ttgaagaggg
    19921 attatttaga gaagacctat actatagatt aaatgtaata aacattcata tgatggaatt
    19981 gaaggacagg caagatgata ttttattgct agcaaattat atattaaaca aattaaatgt
    20041 tgaatacaag gaattgaagg ttttaagtga taaggttaaa aattgtttta ttaactatac
    20101 ttggccggga aatataagag aactacaaaa tgtaataaaa agtgcctatg ctgtaagtga
    20161 tgatatggtc attatgatgt gtgaccttcc atctaagatg gataatatca gcagagtagc
    20221 tcaatgtaat gtggatagca attgttcaat tcatgaaatg gttgagaatt atgaaaagag
    20281 cttaattatt gatgtgttaa ggaagtataa ttggaaatgt tctaaggcag cagaggttat
    20341 gggaatacac aagagtcttt tatataagaa aataaaaaag tatgaaattg aattaaataa
    20401 ttaagataac ttgaattaag gcaatgctaa aaaagcgccc ttattcaaag cgaactgtat
    20461 aaactgtttt atgcagttaa aacgtgtaaa agtagatgtg aagtactaat gtcaatcatt
    20521 agtacttttt tattatattt ataattacta tctatatttc agtatctttt gatatctaag
    20581 attattatgt ttaatttagt atataacaaa accctcttag ttatggacag ttttttatat
    20641 tttaactgtc tactaagagg ggatagttta gtttaaattt agagtaaatt ttatctagca
    20701 tttataaaaa atattttgaa tagctttttt gttatttagt cataattttc tacttcttta
    20761 tcaagttctt tgccacaaga atcacactta tatatagcat tgtatccgta atcatattca
    20821 tcaactattt tcttttgtct aaatacacca ccacactcac ataggaaacc aatttttata
    20881 agtgtaacga taaattgtga ttttataaat tttatatcat tgcatttatc gttagtgtaa
    20941 aatgtataaa tataagagtt atcgcactca tctcttaatt caaatctttc aatatatgtg
    21001 ttatttttta attcctcaaa ttcttcttta gttaaatatg atttttgttg caattcatgt
    21061 aaacgcttaa tttggtccat tatttatatc ctcccaataa ttttagtatt ttaagcttag
    21121 ataaggaatc taagttttat taaatttatc ccaaggaatt atttaaatca tatttagttt
    21181 tattaattac aacatatata aattacacga aaataaaact aattgtaaaa ataatatcct
    21241 gtgaaaacct aaactactaa ccccttacta acccgaaaac taaaaaaaga aaatatatcc
//
LOCUS       DAELLV010000042        20978 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.042, whole genome shotgun sequence.
ACCESSION   DAELLV010000042 DAELLV010000000
VERSION     DAELLV010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20978
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.042"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..1598)
                     /locus_tag="KPD58_001310"
     CDS             complement(<1..1598)
                     /locus_tag="KPD58_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0265092.1"
                     /translation="MKEKIYYNGNIITMEDSICGDAILIKDKIIKKIGTKEEVFALKN
                     KDTEIIDLQGKTLMPSFIDSHSHLIAFATTLKLVPLEDATSFKDIVKKIQDFKESNNI
                     KKGDWIIGFSYDNNFLEENKHPDKSVLDSASSENPILISHASGHMGVANTLGLKQLGV
                     TNETRDPEGGHIGRVEGSEEPNGYLEENAFFNVASKIKQPSSNEIFNSIEKAQNIYLS
                     YGITTAQEGLMEENQFNILKAMANQNKLKMDVVGYVNLKKSKSVADNNREFIKKYINR
                     FKIGGYKIFLDGSPQGKTAWLSRPYENSDDGYCGYPIYKDEEVEKFIDISLKEKMQLL
                     THCNGDAAADQLIDAFEKVLNLKEQSSENHIRPVMIHAQTVREDQIDDMKVINMIPSY
                     FVAHTYYWGDIHIKNLGEDRAFKISPLKTTIEKGLIYTLHQDTPVIAPNMLETVWCAV
                     NRITKKGIQIGENEKISPLDALKGVTINAAYQYFEEDKKGSIKEGKLANLIILDENPL
                     TIDPMKIKDIKVLQTIREGEVLYS"
     gene            1984..2910
                     /locus_tag="KPD58_001311"
     CDS             1984..2910
                     /locus_tag="KPD58_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBG0265093.1"
                     /translation="MSKENKNVRIAVVQASPVIMDLEKTVEKALGLIKEAGRKGANIV
                     VFPEAFIPAYPRGLSFGFVVGSRTMEGREDWKRYYDNSVPVPSATTDLLGKAAQEAGV
                     YLSMGITERDGNDINCTLYCTNLFFSPEGKLIGKHRKLKPTGTERCIWGEGDGSTLTV
                     VDTPYGKMGSLICWENYMPLARTALYAKGVKLYIAPTADSREEWQATMKHIALEGRCF
                     VIGCNQYVEKNMYPTDLNYYKELDAEPEIMCPGGSCIVDPFGKYVAGPIFNKEEMLIA
                     DLDLEKIVLSRLDFDSEGHYSRPDVFELIVHE"
     gene            complement(3030..4028)
                     /locus_tag="KPD58_001312"
     CDS             complement(3030..4028)
                     /locus_tag="KPD58_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HBG0265094.1"
                     /translation="MNKKFKSLYITEEEGNFRRNIVEREVDTLPEGEVLIKVKYSSLN
                     YKDALSANGNKGVTRKYPHTPGIDASGIVESSSCDDFKIGEEVLVTGYDLGMNTSGGF
                     SEYIRVPSSWVVKLPKNLSLKEAMDYGTAGFTAALSVYKFIDSVDKNMGDVLVTGGTG
                     GVAVIATKILIKLGYSVVVSTGKLEEQKEVLLNLGVKDVIHRSEVDDNSGRPLLRPRW
                     AGVIDTVGGNTLATAIKTTNYCGAVTTCGNAGGVDFTSSVYPFILKGITLYGIDSVDC
                     PMNLRLKIWDLLSNEWKLDDLDDMVNVVSLEELNDSIDSIMAGKHVGRTVVDLEKI"
     gene            4132..4569
                     /locus_tag="KPD58_001313"
     CDS             4132..4569
                     /locus_tag="KPD58_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0265095.1"
                     /translation="MEDKYIVHFISKTKASMIKFIENKLSKNGLGELIPTHGNILTAL
                     YENNGILTMKEIAAKIGKDKSTVTVLVNKLINLGYLERQKCTNDKRITYIKLTKKALL
                     IEDTFNSISTQVKETAYHNITENEKQEFLRILKKINDNFKNAD"
     gene            4655..5242
                     /locus_tag="KPD58_001314"
     CDS             4655..5242
                     /locus_tag="KPD58_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBG0265096.1"
                     /translation="MNLKDSKTKENLLRAFAGESQARNRYNISASVAKKEGLYIIESL
                     FNYTADQEKSHAKQFFSKLKEFAGENISISGAYPVDLYDKTAQHLKAAQHNELQEWDD
                     VYKNFGKTAREEGFESIAKLFENIAEIEKVHGDRFGRYATDLEEGTLFRRNEDIQWIC
                     THCGHIHIGKEAPKACPVCLHPQGYFLDFKNSLFE"
     gene            complement(5542..5952)
                     /locus_tag="KPD58_001315"
                     /pseudo
     CDS             complement(5542..5952)
                     /locus_tag="KPD58_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861712.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4004 family protein"
     gene            complement(6070..7188)
                     /locus_tag="KPD58_001316"
     CDS             complement(6070..7188)
                     /locus_tag="KPD58_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG0265097.1"
                     /translation="MAVVEKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIEVY
                     KNNKETFTYKQCSSDLTNLKKELKWLKEPDKFSLQNALKDLDNAYKKFFKEKAGFPKF
                     KSKKINRFSYKTNFTNGNIMYCGQYIKLPKLGMVKVRDKQVPQGRILNATISKEPSGR
                     YYVSLCCTNVDIEVFENTNNQIGLDLGIKEFCISSCGDFIENPKYLKKSLNKLAKLQR
                     ELSRKTIGSLNRNKARLKVARLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNM
                     IRNRKLSRLISDVSWSEFVRQLKYKANWHGRQIVKVGKFFASSQICNKCGYKNEEMKD
                     LNIREWICPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            complement(7190..7597)
                     /gene="tnpA"
                     /locus_tag="KPD58_001317"
     CDS             complement(7190..7597)
                     /gene="tnpA"
                     /locus_tag="KPD58_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBG0265098.1"
                     /translation="MEVTHGRGYVYLVQYHIVWCVKYRHPVLTNEIEEDLINILNKIA
                     EDNKFQILECNSDKDHIHLLINCSPQHYIPDIIKALKGVSARLLMKKHGEQLKKKLWG
                     GHLWNPSYFVATVSENTEEQIRKYINNQKKQVK"
     gene            complement(8166..8375)
                     /locus_tag="KPD58_001318"
                     /pseudo
     CDS             complement(8166..8375)
                     /locus_tag="KPD58_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861712.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4004 family protein"
     gene            complement(8477..9025)
                     /locus_tag="KPD58_001319"
                     /pseudo
     CDS             complement(8477..9025)
                     /locus_tag="KPD58_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426706.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9384..11294)
                     /gene="phnW"
                     /locus_tag="KPD58_001320"
     CDS             complement(9384..11294)
                     /gene="phnW"
                     /locus_tag="KPD58_001320"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HBG0265099.1"
                     /translation="MKKIYGEKIKAVVFDWAGTTVDYGCFAPLNVFIEIFKRRGIDVT
                     MEEARKPMGKLKIDHIREMCEMDRIKNIWSDKFGKVPTEDDVNELYAEFEPMLFETLE
                     EYTTPIPHVVETIEKLRKNGLKIGSTTGYTREMMNIVEPNAAKKGYSPDFLVTPSEVS
                     QGRPYPWMCYKNAEALGVSPMSSMVKVGDTISDVKEGVNAGMWSVAVIKGSSELGLTQ
                     EEVENMDKEELKAKMSIVSKKFKEAGAHFVIETMAELEDILIKIENETIKSDFVPEND
                     YILLTPGPLSTTKSVRASMLKDWCTWDVEYNNLVQDVRRRLVSLATQNTDKYTSVLMQ
                     GSGTFSVEAIIGSTISKDGKLLVIANGAYGKRMKDICNYLNIQFVDCTFKDIEAVDLN
                     VVENLLKENKDITHISMVHCETTTGRLNPIQEVGKLAKEYNKIYIVDAMSSFGGIEID
                     VEDFNIDFLVSSSNKCIQGVPGFGFIIANKEKLSKCKGIAKSLSLDVYAQWETMEKNN
                     GKWRFTSPTHVVRAFYQALLELEEEGSVEKRYARYKENQFTIASRLKSLGFDTLVNDN
                     AQSPVITTFLYPKNAKFEFMEFYTYLKDNGFVIYPGKLTDIDTFRIGSIGEVYPTDME
                     RLADVIEKFINR"
     gene            complement(11573..12358)
                     /locus_tag="KPD58_001321"
     CDS             complement(11573..12358)
                     /locus_tag="KPD58_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBG0265100.1"
                     /translation="MSLNSIDRKKEILKLLDSKGKVNTKELVKRLEVSSETIRRYLEE
                     LENENKLKKVYGGAIKINYGVIEPEHIKRNNTNLDKKQSIAKYAAKFVNDNESIIIDE
                     GTTNLQIVDYIISVNNLKIITNSYPVLSKLISYENQGLFDGEVIFIGGKVNLKHQRTA
                     CPMSIDTMKNLYVDKAFISSEGILDSFGISSIDQNKALLSKEYIKNSKESFVLCDSSK
                     IGIASMYKIADLEELDFVICDVEPPNEWKDKLNEINITWIEAK"
     gene            complement(12575..12805)
                     /gene="dltC"
                     /locus_tag="KPD58_001322"
     CDS             complement(12575..12805)
                     /gene="dltC"
                     /locus_tag="KPD58_001322"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HBG0265101.1"
                     /translation="MQETVIEIFEDVLGTDEIREDLDLNLFETELLDSLAIIEVLLEI
                     ENRLGIELQPTDLERKDMSTVNNLVKFLETRK"
     gene            complement(12824..13972)
                     /gene="dltB"
                     /locus_tag="KPD58_001323"
     CDS             complement(12824..13972)
                     /gene="dltB"
                     /locus_tag="KPD58_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HBG0265102.1"
                     /translation="MIFSQYGDYFYLYILLLTSIPAVILGLMGKNIKYYGMLASLFMI
                     FLIVGIDVQLKYLVIFIILEVIIVKGYEYVRRKTKNKYIYWGFLFASMLPIIINKISP
                     VTSFGIIGFIGISYLNFRTIQMVIEIYDGAIKEVKISKMLYFMLFFPTLSSGPIDRSR
                     RFEQDLEKQISRKEYIEEYLLPGIKNIVMGVGYKFVIAFLINTYWVSRIPKDMSFINI
                     LSYMYAYSLYLFFDFAGYSLFAIGTGYIFGIQVPINFDKPFISKDMKEFWTRWHISLS
                     RWFGDYIFSRFVMSSMRKKRFKKRTTAAHVAQMITMITMGFWHGLTWCYVAYGVYQGL
                     ALVLTDIYQRKSKFYKKHKKDKWFERVQIFITFHIVCFGLLIFSGYLA"
     gene            complement(13972..15486)
                     /locus_tag="KPD58_001324"
     CDS             complement(13972..15486)
                     /locus_tag="KPD58_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase"
                     /protein_id="HBG0265103.1"
                     /translation="MKIIEGIKKYSNTDRTALMCNGDKLSYKDLNEYSDAISVFLKDV
                     YKEEDTPIVIYGNKENMIMACMIGALKSGRAYVPLDISFPIDRVFEVTKEIKPKVLFN
                     FSDERNFGDINVIDMDKLNDIINEYQGKSLDKENWVKDDENAYILFTSGSTGKPKGVQ
                     ISSNNLDSFSDWISPYLNIDGSEKVIMNQPAYSFDLSVTTIYPGLIHGATLFSISKDV
                     LADYRELFRQFSISDIAVWVSTPSFAGVCITEKEFNSKMLPNLESMIFIGEALSKNLT
                     KELMSRFPNTRIINGYGPTEATVGVSVNDMTQKAIDDEKSLPVGYPMSNCKIKILDED
                     GNELKENEKGEIIIIGPSVSKGYFNNKEKTDEVFFYDEIDGVKWRAYKTGDMGYLLDG
                     NIYYCGRKDFQIKLNGFRIEIEDIENNLRKVHNVKNAVVLPVYKDEKIAYLKGIVELN
                     EKNDLSNIKNGMIIKKELGKYIPSYMIPRNISIISEFPTNINGKIDRKKLMEEI"
     gene            complement(15607..16797)
                     /gene="dltD"
                     /locus_tag="KPD58_001325"
     CDS             complement(15607..16797)
                     /gene="dltD"
                     /locus_tag="KPD58_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HBG0265104.1"
                     /translation="MRKLIYFITPFIIGVVFLFGLDKFLDSKTDELLREKNLLPIMDD
                     TLSDIKDKGVTANNHFLREKDIMILGSSELSNSTKQHPKYYFNTNRSKNKVFAIGRAY
                     TQTLQDAAILGSMNPNIDNKKVVLLISMQWFMEKDGVTSHHYQSRFSPIQFYRFLDNP
                     KISKQNKIEYAKKSSKLLWGSDEYKAEALYAKLYEPKTFPEKAEKVLLEPYFQGRKYC
                     IALKEKGILYKRLIKLDKKRATKRKRPINWSYERKKAIEDAKKRVGKNPLNIDKYYYK
                     EHFKDGIEQYKGRDKDVNLLTSKEFENYKLMLNVCTDLGIKPVVVLIPSMDKFYNLTG
                     ISEKERNQYYDKAQKIAESKGFEVLNLKDKGSDKYYLRDVMHLGTKGWVDVCERLFKI
                     FKEQ"
     gene            complement(17085..18104)
                     /locus_tag="KPD58_001326"
     CDS             complement(17085..18104)
                     /locus_tag="KPD58_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBG0265105.1"
                     /translation="MMGKIRFGIVGTSNIANIFLRAASRVKDFELVAVYSRNLEKARE
                     FGTLYGASIFFDDLEQMAKSKDIDAVYIASPNALHSKQTITCLKHKKHVLCEKSLASN
                     LKEVKSMIKTAKDNNVLLMEAMRITCVPNFKAVKENLYKIGKIRRFFGSYCQYSSRYD
                     KYKNGIIENAFKKELSNGALMDIGVYCIHPMVNLFGAPKTVKAVSHILQTGVDGEGSA
                     VFQYDDMDAIIQYSKVADSYIPSEIQGEEGSIIIEKLNLFEKAIIKYRDGREEDISIP
                     KEKAEEKPREIEGMYYELVEFISLINNNKIESNINSHENSIIVMEIMDEIRRQSSIVY
                     PADSI"
     gene            complement(18280..18819)
                     /locus_tag="KPD58_001327"
     CDS             complement(18280..18819)
                     /locus_tag="KPD58_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HBG0265106.1"
                     /translation="MDNVGSLLYFLFNFFLYGFIGWIIENLYSYYKQGHFQEDGFLNG
                     PFKPMYAIAMSIIIEVSRFIDPNNIFALLLLCLVVPTVVEYITGILMKVYFNKVYWDY
                     SDYKYNSRGIVCLKFSVYWTILTFIGVRYFQVYIVDSLYSLIIPYWPIVVTILLVTLF
                     VDEILTIKFLRGNKDIVER"
     gene            complement(18920..19600)
                     /locus_tag="KPD58_001328"
     CDS             complement(18920..19600)
                     /locus_tag="KPD58_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBG0265107.1"
                     /translation="MQNVEGIIFDMDGVLFDSERISLEFWMETFEKYGYTMTKEIYTS
                     VMGRNRKGIIEGLTDIYDSSVPIIDLYDEKTKNMIEFMERKGAPIKLGVNELISFLKE
                     NGYKMAVATSTKRERAVKRLAKANLKDYFDAIVCGDDVVNSKPNPEIFLKAAKKINVN
                     PKNCIVIEDSPMGVEAAYNGGIRCINVPDLKEPDEQIKSQSHKILENLLEVREYLKSL
                     NSKECHNL"
     gene            complement(19629..20918)
                     /locus_tag="KPD58_001329"
     CDS             complement(19629..20918)
                     /locus_tag="KPD58_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBG0265108.1"
                     /translation="MRELLKVTLIVMLVLINAFFVACEFAMVKLRSSRIDTMITEGNN
                     NAKRCKIIKDNLNSYLSACQLGITLCSLALGWMGESTVKELILPIVSVFNLSESIIYT
                     ISISISFLIITMMEVVIGELVPKALALYNTEKIMLSTSFLLVGFYKLTYPIIYFFNLS
                     TDLFLKPFGYSQADEVDEPHTGDEIRLLVEESYKSGLIDESEQRLVDNIFEFEEKKIR
                     EIMVPRTDMVCIYESDSEEKILAILKEEGVTRYPVCRKNKDDILGFVHIRDLYNQKIN
                     ENKIELEEILRDIIYISENLTIDKALERIRKEKLQLAIVVDEYGGTSGVVTIEDILEE
                     IVGEIQDEFDKEETHIKKIGAGSYLVDGTESINYINKYFGLDIEHDGFDSIGGWISYM
                     LGSNIKVNQSVTFENYNFTILELDKLRVVKLIIKSVI"
BASE COUNT         6821 a         3640 c         2236 g         8281 t
ORIGIN      
        1 aaagaataca atacttctcc ttctcttata gtctgcaata ctttaatatc ttttattttc
       61 atagggtcga ttgtaagtgg gttttcatct aatattatca aattagctaa tttcccttct
      121 ttaattgaac cttttttatc ttcttcaaaa tattgatatg ctgcatttat agtaacaccc
      181 tttaaagcat ctaatgggct tatcttttca ttttctccta tttgtatacc tttttttgta
      241 attctattga cagcacacca tactgtttcc agcatattag gtgctattac tggtgtatcc
      301 tgatgaagag tgtaaattaa tcccttttct atagttgttt taagtgggct aatcttaaac
      361 gctctatctt ctccaaggtt tttgatatga atatcacccc aataatatgt gtgagctaca
      421 aagtatgaag gtatcatatt tattactttc atatcatcaa tctggtcttc tcttactgtt
      481 tgagcatgta tcataactgg tcttatatga ttttcactac tttgctcttt taagtttaaa
      541 actttctcaa acgcatctat caactggtct gctgctgcat ctccattaca atgagttaag
      601 agttgcatct tttcttttag tgaaatatct ataaactttt ctacttcttc atctttgtat
      661 ataggatatc cacaataacc atcatctgaa ttttcatatg gtctactcaa ccatgctgtc
      721 ttaccttgtg gagaaccatc taagaaaatc ttataacctc ctattttaaa tctatttata
      781 tattttttta taaactccct attattatct gctacagact tacttttttt caaatttaca
      841 tatcctacaa catccatttt taatttattt tgatttgcca ttgcttttaa aatgttaaat
      901 tgattttctt ccattaaacc ttcttgagct gttgtaatac cataacttag atatatgttc
      961 tgtgcttttt caattgaatt aaaaatctca tttgatgatg gttgttttat tttagatgct
     1021 acattaaaaa atgcattttc ttctaaatat ccatttggtt cttcactgcc ttctactcta
     1081 cctatatgtc caccttctgg gtcacgtgtt tcattggtaa caccaagctg ttttaacccc
     1141 aatgtatttg caactcccat atgaccagaa gcatgtgaaa ttagtattgg attttctgaa
     1201 gatgcactat cgagtacact cttatctgga tgcttatttt cttctaaaaa attattatca
     1261 tatgaaaatc caattatcca gtctcctttt tttatattat tcgactcttt gaaatcctgt
     1321 atttttttta ctatgtcttt aaaactggtt gcatcttcta aaggaactag ctttagagta
     1381 gtagcaaaag ctatcaagtg actatgtgaa tctataaaag atggcatcaa tgtcttacct
     1441 tgtaaatcta taatttctgt atctttattt ttcaaagcaa atacttcttc ttttgttcct
     1501 atctttttta ttattttgtc ttttataaga atagcatctc cacatattga atcttccatt
     1561 gtgatgatat ttccattgta ataaattttt tctttcataa aattctcctt acgttgtcta
     1621 ctttaataaa tttttttcat tattttctgc ttaattatat tatcttcttt ataaactttt
     1681 tctttagagt ttaatcatat aaaagtttta tttttaatta ttgtttgcca agaagtaaat
     1741 actattcatt gtaaataaga caaattaata ggctttatac taattgaaaa aatattataa
     1801 gttgttaaat ttaaatatct aaactcaatt atatctgtac aattgtaatt ttttaaatca
     1861 tgatgtatta ttttaaatat acagttacat tattattcac aatataagta tcttagttct
     1921 caaataacat ctcatgtaat ctattcaatg caaaaaattt aaatattttt taggaggttt
     1981 tttatgagca aggaaaataa aaatgtaaga attgctgttg tccaggcgtc accagtcata
     2041 atggatttag aaaaaactgt tgaaaaagca ttaggattaa ttaaagaagc tggtagaaaa
     2101 ggggcaaata tcgtagtttt ccctgaagca ttcatacctg catatccaag aggtctttca
     2161 tttggatttg ttgttggaag tagaacaatg gaaggtagag aagattggaa aagatactat
     2221 gataattctg taccagttcc tagtgctaca acagatttat taggaaaagc agcacaagaa
     2281 gcaggagtat atctatcaat gggtataaca gaaagagatg gtaatgatat aaattgtacc
     2341 ctttattgca caaacttatt tttctctcca gaaggtaagc taattggtaa acacagaaaa
     2401 cttaaaccta caggaacaga aagatgtatt tggggagaag gagatggaag tactcttaca
     2461 gtagttgata ctccatatgg aaaaatgggt tcgttgattt gttgggaaaa ttatatgcca
     2521 ttagctagaa cagctcttta tgctaagggt gtaaaacttt acatagcccc tactgcagat
     2581 tcaagagaag agtggcaagc aactatgaag catattgctt tagaaggaag atgttttgta
     2641 attggatgca atcaatatgt tgaaaaaaat atgtatccaa ctgaccttaa ctactataaa
     2701 gagctagatg cagaaccaga aattatgtgt ccaggaggaa gttgtatagt tgaccctttt
     2761 ggaaaatatg tagcaggtcc tattttcaat aaagaagaaa tgctaatagc tgatttagac
     2821 ctagaaaaaa tagtacttag tagactagat tttgattcag aaggtcacta ttcaagacca
     2881 gatgtatttg aattaattgt tcatgaataa tctttttaca caaacatcta cataaatctt
     2941 tttacataaa aaaatgagat actcataagt aaatttcaag tatctcattt ttgcttaaaa
     3001 cctataatcc taattataaa tttattcacc tatatttttt ctaaatcaac tactgttctt
     3061 ccaacatgtt ttcctgccat tattgaatct atactatcat ttaactcttc caatgatact
     3121 acatttacca tatcatctaa atcatctaat ttccattcat ttgataataa atcccatatt
     3181 tttaatctta agttcattgg gcaatccaca gaatctatac catataaagt aattcccttt
     3241 aagataaatg gatatactga acttgtaaaa tcaactcctc ctgcattacc acaagttgtt
     3301 actgctccac aataattagt tgtctttatt gctgttgcta gagtatttcc tcctactgta
     3361 tctattactc cagcccatct tggtctaagc agaggtcttc ctgagttatc atccacttct
     3421 gacctatgta taacatcttt aacacctaag tttagtaata cttctttttg ttcttctaat
     3481 tttcctgttg aaactacaac tgaataacct aatttaatca atattttagt tgcaattaca
     3541 gcaactcctc cagtaccacc agtgactaat acatctccca tattcttgtc tacagaatct
     3601 ataaatttat acacagataa agctgctgta aaccctgctg taccataatc cattgcttct
     3661 tttagtgaaa gatttttagg aagtttaact acccaactag aaggaactct tatgtattct
     3721 gaaaaaccac cagaagtatt cattcccaag tcataacctg ttactaaaac ttcttctcca
     3781 attttaaaat catcacaact actagattct actataccag atgcatcaat tcctggtgta
     3841 tgaggatact ttctcgtaac acccttatta ccatttgcag aaagtgcatc cttgtaattt
     3901 aaagaagaat attttacttt tattaaaact tcgccttctg gcaatgtatc cacttctctt
     3961 tcaactatat ttcttctaaa atttccttct tcttctgtaa tatatagtga tttaaatttt
     4021 ttattcataa atttcctccc aatacattta gttttatgtt aaactaatta tagtttataa
     4081 atagttttat gtcaaactaa tttaaataga ttaaagattg gagaataatt tatggaagat
     4141 aaatatattg tacatttcat aagtaaaaca aaggctagta tgattaaatt cattgaaaat
     4201 aaactcagta aaaatggttt aggtgagtta atacctactc atggaaatat tttaactgca
     4261 ctttatgaaa ataatggaat acttacaatg aaagaaattg cagctaaaat aggaaaagat
     4321 aaatccactg ttacagtgtt ggttaataaa cttataaacc taggatattt agaaagacaa
     4381 aaatgtacaa atgacaaacg tatcacgtat ataaagctta caaaaaaggc actgcttata
     4441 gaagatactt ttaatagcat ctcaacacaa gttaaagaga cggcatacca taatattaca
     4501 gaaaatgaaa aacaagaatt tcttagaatt ttaaaaaaaa ttaatgataa ttttaaaaat
     4561 gctgactaaa ttgagcataa ttatatctcc tattggaata ataaataaaa agaatcaaat
     4621 attgtaaaaa tttattgtga tagagaggta gaatatgaat ttaaaagata gtaaaactaa
     4681 agaaaattta ttaagagctt ttgcaggtga gagtcaagct agaaatagat acaatatttc
     4741 tgcatctgta gcaaaaaaag aaggattata tataatagag tctttattta attacactgc
     4801 tgaccaagaa aaatcacatg caaaacaatt cttttctaaa ttaaaagaat ttgctggtga
     4861 aaatataagt atatcaggag cataccctgt agatttatat gacaagacag cacaacacct
     4921 taaagctgca caacataatg agcttcaaga gtgggatgat gtatataaaa actttggaaa
     4981 aacagcacgt gaagaaggat ttgaatctat agctaagtta tttgaaaata tagctgaaat
     5041 agaaaaagtt catggtgata gatttggtag atatgctact gatttagaag aaggtacttt
     5101 attcagaaga aatgaagata tacaatggat atgtacacat tgtggccata tacatatagg
     5161 taaagaagct cctaaagctt gtcctgtatg tcttcatcca caaggatatt tcttagattt
     5221 taaaaactca ttatttgaat aaactaaggt aatcattgta tttaaacaat ttactatatt
     5281 tatatcaagt atttttataa atactgtatt atcttaataa caataaaatg aaccattgat
     5341 attactataa gtagaaaatg tagtttaaat acaatttata gtagttcatc atggttcatt
     5401 ttattgttta ctaatcccta taacttactt aaatctatct ttaactttta catactcaca
     5461 taaatcctca tctctaaact aaatatttaa ataaagtgta ttttaagtag attattataa
     5521 tacattatat aagataatac actatgataa ctttatactt atatcattta tttcttttgt
     5581 caagtcaacc tcaataatct taactacatc tttttcaaac tttacttttt tataatccag
     5641 acatcctact acgaatggaa ctccaaactt tctaaacaaa taaatctttc cactttcatt
     5701 gtatattgat gtaaaatttt cttctattat attcactatc aattttaact cttcaaaagt
     5761 tattatagat ttaactaaaa acttctctat tatgctcaat ataagcagtt cttttttacc
     5821 tatgctttgt tcttttttat ataaattttg aaatatttct ttggtataat tacatataac
     5881 ttctttactg agaataaaat caatgtcaaa ttctttatta atatcctttt tcataaacat
     5941 atttgcaata tcctgaacct cccatgacta aagtcacagg gttcctgctt catagaattt
     6001 tgcatactaa aatatgtctt actaattctc cacaggctat ccccgtagtt cctacggttc
     6061 ttacataagt tatttatttt gaattgttat tagtcttaat ccttctttta atatatttat
     6121 acttgcattt atatctctat cgtgagtttc attacaacta ggacaaatcc attctcttat
     6181 atttaagtcc ttcatttcct cgtttttata cccacattta ttgcatattt gtgaacttgc
     6241 aaagaattta cctaccttta caatttgtct accatgccaa ttagccttgt attttaattg
     6301 acgaacaaat tctgaccatg acacatcaga aattaagcga gatagtttac gatttctaat
     6361 catattcttt acttgtaaat cttctatgca aataatatca ttctctttta ttaatttagt
     6421 agatagtttt tgtagaaaat catttctttg attagctata tgttcttgaa gtcttgcaac
     6481 ttttaatctt gctttatttc tatttaaact accgattgtc tttctcgata attctctttg
     6541 taatttagca agtttgttta atgacttctt aagatactta ggattttcta taaagtctcc
     6601 acaacttgag atacaaaatt ctttaattcc taaatctaaa cctatctgat tattagtatt
     6661 ttcaaatact tcaatatcta cattagtaca gcataatgat acataatatc taccacttgg
     6721 ttctttagat atagtagcat taagtattct accttgaggt acttgtttat cccttacctt
     6781 aaccatacca agtttaggta gttttatata ttgaccacaa tacataatgt ttccatttgt
     6841 aaagttagtt ttatatgaaa atctattaat tttctttgat ttaaatttag gaaatcctgc
     6901 tttttcttta aagaattttt tatatgcatt atctaaatct tttaaggcgt tttgtagtga
     6961 aaatttgtca ggttccttaa gccattttaa ttcttttttt aaattggtta aatcagaact
     7021 acactgttta taagtaaaag tttctttatt gtttttataa acttctattc ttttagcaag
     7081 atacttatta tatacaaatc tacaacatcc aaaagttttg ttaattaact cttgttgttt
     7141 tttatttgga tacattctaa acttataagc tttttccact actgccacct cactttactt
     7201 gttttttctg attattaata tattttctta tctgttcttc tgtattttct gatacagtag
     7261 caacgaaata actaggattc caaagatgtc ctccccataa tttctttttc aactgttctc
     7321 catgtttttt cattaacagc cttgctgata caccttttaa tgcttttatt atatctggta
     7381 tatagtgttg tggtgaacaa tttattagta aatgtatgtg atctttatca ctattacatt
     7441 ctaatatttg aaatttatta tcttcagcta ttttgtttaa tatattaatt aaatcttctt
     7501 caatttcatt tgttaatact ggatgtctat attttacaca ccatactata tggtattgta
     7561 ctaaatacac ataccctcta ccatgagtca cttccatttt tcatcaccca acataatatt
     7621 aacatagtaa ttacttaaaa caagtattct taaggttttt atatttattt ttaaataaca
     7681 tacgtaagaa acgcaaatct taacttttat catttttgag gttgtcgttc acataagttc
     7741 gctactacct atgcagttct cttatgaact tcttacactt tcatgcaagc acagactata
     7801 tcttatcctt cggcattatc cgttaaggtc tacccacttc cactaccgat agcttgtagt
     7861 gtacttccct caagaggaat agtcgttgaa gtttttccta ttcggagctt acctgctgat
     7921 tgcccatttt aaagtactta ggatttaacc ttataccatc taaccaattt tttctacttt
     7981 cgtaactttc acacttaggt ttatttcatc cttatgtttt agtttggtta gctttagggg
     8041 tttccagcaa ttcaagtagt attggatagg ttctatccta tctctacata taagtttccc
     8101 tatatgctga ctattttagc tactaactca tgactaaagt cacaagtgtg catagctatt
     8161 ttttaatcaa gagaagtctc ttctttcata gacaatatta actctattct ttctagtatt
     8221 ttatctcttg gaaagaaagt ctcttgacct gtaaaagctg atttttttat aaaccattcc
     8281 tctggtatta tgttttttct tttccatcta tataattgtc cataagatat attagttttg
     8341 ctcaataaat ctttcttaga tattaattct tccatagctc ttctagccca cctttatact
     8401 gttttttaca gtagaattat ctgaaactga taaattatca ctgtattcaa tatttccaac
     8461 atcataatta tctactagag atatgctttt tttctagcat acgaaatttt ttgcaataat
     8521 ctccatgccc cttttttata tttatactac ttgctccaac ttcattaaaa ttagaagttc
     8581 catttaaact aatattaact tcttcacaat tcaaaaatcc attacactcc aagattccac
     8641 ttaaattgat attttcacaa tctatattct catttgcttt taaatatcct tctccatgaa
     8701 taatatccga ttttatactt ccttctacat taataattcc actagtatca atttcaacag
     8761 aattaatatt tcctgtacat ctaagaagtc ccgatatctc aacttttgat gccagtatgt
     8821 ttccttctac tttacttaaa ccagatgtaa aaaattcatt acattctgtt gtcccaaaaa
     8881 cttttgctga accagaagaa tcaaatttac tacattttac acttccttct attttgcctg
     8941 ttcctgatat attaacatta tcaaaaattc cacctttaca ttttccaaca ccatcaaatc
     9001 ttagtgaact taattcttca ttcataatga tatcctttct ttaccgtatt ttatctttaa
     9061 acctaatata acaaaacatt gttacgttgt caagtatgat aataatactt aataaagcaa
     9121 ctatcattta ttttctataa ataggcataa aaataatagg gaaatgcttt atttaaacta
     9181 taaatctaat ttcttagatt gtggtttaaa taaaatatat ccctattact caatgtttat
     9241 atattcattg tataaacttc taaaatataa gtctttaaat tctactgtca atacttttta
     9301 tacactgttt gtgctatcat ctcacacacc taaaggagta ggctttgcgc actattctgt
     9361 aacctacaag acactatctt cttctatcta ttgataaatt tttctattac atcagctagt
     9421 ctctccatat cagttggata gacttcacct atgctaccta ttctaaatgt atctatatca
     9481 gtcaatttac ctggatagat aacaaatcca ttatctttta aataagtata aaattccata
     9541 aattcaaact ttgcattttt aggatataag aaagttgtta ttacaggtga ttgagcatta
     9601 tcattcacca aagtatcaaa gcctaaagat tttaatcttg aagctattgt aaactgattt
     9661 tctttatatc tagcatatct tttttcaaca gaaccttctt cttccaattc taataaagct
     9721 tgataaaaag ctcttacaac atgtgtaggt gaagtaaatc tccatttacc attatttttc
     9781 tccatagtct cccattgcgc atacacatca agagataatg atttagctat acctttacat
     9841 ttagataatt tctctttatt agctattata aaaccaaatc caggtactcc ttgtatacat
     9901 ttgttagaac tactaactaa aaaatctata ttaaaatctt caacatcaat ctcaattccc
     9961 ccaaaactac tcatagcatc aactatataa attttgttat attcttttgc aagtttccct
    10021 acctcttgta taggatttaa tctacctgta gtagtttcac agtgaaccat tgatatatgt
    10081 gttatatcct tgttttcttt taataaattt tctactacat ttaaatccac tgcttctata
    10141 tctttaaatg tgcagtcaac aaattgtatg ttaagataat tacatatatc tttcattctc
    10201 tttccatatg ctccatttgc aattacaaga agtttaccat cttttgaaat agtacttcct
    10261 attatagctt ctactgaaaa agtcccactt ccttgcatta atacacttgt gtatttatct
    10321 gtattttggg ttgctagaga tactaatctt cttctaacat cttgtactag gttattatat
    10381 tcaacatccc aagtacacca atcttttaac atagatgctc ttactgattt agtagtagat
    10441 agtggtcctg gtgtaagtaa tatgtaatca ttttctggta caaaatctga tttaatagtt
    10501 tcattctcta tttttattag tatatcttct aactcagcca tagtttctat tacaaaatgt
    10561 gcacctgcct ctttaaattt ctttgaaact atactcatct tagcttttaa ttcttcctta
    10621 tccatgttct ctacttcttc ttgtgtaagc cctaactcac ttgaaccttt tattacagca
    10681 acactccaca ttcctgcatt tacgccttct tttacatcac ttattgtatc tcctactttt
    10741 accatagaac tcataggaga cacccctaaa gcttcagcat ttttatagca catccaagga
    10801 tacggtcttc cttgagatac ttctgatggt gttacaagaa aatcgggtga gtatcccttc
    10861 tttgctgcat ttggttcaac tatgttcatc atctctcttg tataacccgt tgtagaacct
    10921 atttttaatc cattttttct caacttttca atagtttcaa caacatgagg aattggagtc
    10981 gtatactctt ctaatgtctc aaataacatt ggctcaaatt cagcataaag ttcatttaca
    11041 tcatcttcag ttggcacttt accaaactta tctgaccaaa tattctttat tctatccatt
    11101 tcacacattt ctcttatatg gtctattttt aattttccca taggttttct tgcttcttcc
    11161 attgttacat ctatacctct tcttttaaat atctctataa atacattaag aggtgcaaaa
    11221 catccataat ctactgttgt tccagcccaa tcgaatacta ctgcttttat tttttctcca
    11281 tatatctttt tcatatttat agcctccata gattcttttt agaatttttg tgtttttaat
    11341 tgtttttact tttaaagtat aactttcata tgttaattat acatcatatt taggttaaat
    11401 atatattaaa tttctgtttt tagttgtatt tagttgtttt tattttgtat catattccca
    11461 taaaatccta agttacaatc taaaatttac tttctaatct taatctatga actgactata
    11521 ttttatgaat ttagttgtat tgagcatagt tgtattatat aaaactaagg gtttattttg
    11581 cttctatcca tgttatattt atctcattta acttatcctt ccattcattc ggaggttcta
    11641 catcacaaat aacaaaatct aattcctcta aatctgctat cttatacata ctagcaattc
    11701 ctattttaga actatcacat aatacaaaac tttcctttga attttttata tattcttttg
    11761 acaacaatgc cttattttgg tctatactac taattccaaa actatcaagt ataccttcac
    11821 tagatataaa agctttatct acgtataagt ttttcatagt atctattgac attggacatg
    11881 ctgtcctttg gtgttttaaa tttacttttc caccaataaa tataacttct ccatcaaaca
    11941 aaccttgatt ctcataagaa atcaattttg atagaactgg ataagagttt gtaataattt
    12001 ttaaattgtt tacagatatt atataatcta caatctgcaa attggtagta ccttcatcta
    12061 tgataattga ctcattgtca tttacaaact tagctgcata tttagcaata ctttgctttt
    12121 tatctaggtt agtattgttt ctctttatat gttctggttc aatcactcca taatttattt
    12181 ttatagcacc accatatacc ttttttaatt tattttcatt ttctaattct tccaaatatc
    12241 ttcttattgt ttctgatgat acttctaatc tttttactaa ttctttagtg tttaccttcc
    12301 cttttgaatc taataattta agtatttctt tttttctatc tatcgaattt aaagacattc
    12361 attttcctcc taaatataat atatttttct cctataatat tacaatgata cctaagattt
    12421 atgaaatcat atttatgtat taaatttttt acttaattaa agttattttc aaaagacttt
    12481 gtattatcat taaaaaagtc catcaaatat tagtatcaga tggactttgt aaaattttat
    12541 tttataaatt aatatttaaa gtctaactac taatttattt tcttgtctcc aagaatttta
    12601 ctaaattatt tacagtagac atatcttttc tctctaaatc agttggttga agctctatcc
    12661 ctaatctatt ttcaatttca agtaaaactt caattatagc taatgagtct aaaagttccg
    12721 tctcaaataa attaaggtct aaatcttctc ttatctcatc agtccctaat acatcttcaa
    12781 atatttctat aactgtttct tgcataaatt ttcctcctta aaattaagct aaataacctg
    12841 agaatatcag caatccaaaa cacacaatat ggaatgtaat gaaaatctgc actctctcga
    12901 accatttatc ttttttatgt tttttataaa atttagattt tcgctgatat atatcagtta
    12961 acacaagtgc caatccttgg tatacaccat aagcaacata acaccaagtt agaccatgcc
    13021 aaaatcccat tgttatcatg gttatcatct gagccacatg agcagcagta gtccttttct
    13081 taaatctttt ctttctcatt gatgacataa caaatctaga aaaaatataa tctccaaacc
    13141 atcttgaaag acttatatgc catcttgtcc agaactcttt catatctttg ctgataaatg
    13201 gcttgtcaaa gtttataggt acttgtattc caaatatgta ccctgtacct atagcaaata
    13261 aactatatcc cgcaaaatca aagaacaggt ataaactata tgcgtacata taacttaaaa
    13321 tatttataaa actcatatct ttaggtattc ttgataccca atatgtattt atcaaaaatg
    13381 ctataacaaa cttataacct actcccatga caatattttt tattcctggc agtaagtatt
    13441 cttctatata ctcttttcta gagatttgtt tttccaagtc ctgttcaaat cttcttgacc
    13501 tatcaatagg tccagaactt aaagttggaa aaaataacat aaaataaagc atctttgaaa
    13561 ttttaacttc ttttatagct ccatcataaa tttcaataac catttgtata gttctgaaat
    13621 ttaagtatga tattccaata aatccaatta ttccaaaaga agtaactgga gatattttat
    13681 ttattattat tgggagcatt gatgcaaaca agaatcccca atatatgtat ttatttttag
    13741 tttttcttct tacatattca tatcctttaa ctattattac ttcaagaata ataaatatta
    13801 ccaagtactt tagttggaca tcaattccta ctattaagaa tatcataaac aaacttgcca
    13861 acataccata atactttata ttcttgccca ttagacctaa aattactgct ggtattgatg
    13921 ttaataataa tatataaaga taaaaataat ctccatattg tgaaaaaatc attaaatttc
    13981 ctccataagt ttctttctgt ctattttacc attgatattt gttggaaact cagagattat
    14041 tgaaatattt cttggaatca tatatgaagg tatatacttt ccaagttcct tttttattat
    14101 catcccattt ttaatattac ttaaatcatt cttttcattt aactcaacaa taccttttaa
    14161 gtaagcaatt ttttcgtcct tatatactgg taaaactact gcatttttta cattatgtac
    14221 ttttctcaaa ttattttcta tatcttctat ttcaattcta aatccattta atttaatctg
    14281 aaaatctttt cttccacaat aatatatatt tccatctaaa agatatccca tatctccagt
    14341 tttataagct ctccacttta caccatctat ttcatcatag aaaaatactt catcagtctt
    14401 ttctttatta ttgaagtaac ctttagaaac tgatggtcca attattataa tttcaccctt
    14461 ttcattttct ttaagctcat ttccatcttc atcaagtatt tttattttac aatttgacat
    14521 agggtatcca actggaagac ttttttcatc atctatagcc ttttgagtca tatcatttac
    14581 acttacacca acagtagcct cggttggtcc atatccattg ataattcttg tgtttggaaa
    14641 tctactcata agttctttag taagattttt tgaaagtgct tctcctatga atatcattga
    14701 ctcaaggtta ggaagcattt tactattaaa ctctttttca gttatacata ctcctgcaaa
    14761 agaaggagtt gagacccata cagctatatc tgagatacta aattgtctaa atagttctct
    14821 atagtctgct aatacatctt ttgatattga aaataatgta gctccatgga taagaccagg
    14881 gtatattgtt gtaactgaaa ggtcaaaaga atatgctggt tggttcatta ttactttttc
    14941 acttccatct atgtttagat atgggcttat ccaatctgaa aagctatcta aattgtttga
    15001 acttatttga accccttttg gctttcctgt acttcctgaa gtaaatagta tatatgcatt
    15061 ttcatcatct tttacccagt tttctttatc taatgatttt ccctgatatt catttattat
    15121 atcatttaat ttatccatat caattacatt gatatctcca aaatttcttt catcactaaa
    15181 gttaaataat acctttggtt ttatctcttt agtgacttca aaaactctat caattggaaa
    15241 actaatatca agaggcacat acgctctacc tgattttaaa gctcctatca tacatgccat
    15301 tatcatgttt tctttatttc cataaattac tataggagta tcttcttctt tgtatacatc
    15361 ctttagaaat actgatatag catctgaata ttcatttaaa tctttataag aaagtttatc
    15421 tccattacac attagtgctg ttctatctgt atttgaatat ttttttattc cttcaataat
    15481 tttcatataa gaatgctcct ttaaaatccg ttataaataa atggcaattc tttaaatgga
    15541 gtaaatgtta gaactcctat cgccaatatt ataaccatag ttaaacaaat actagttaac
    15601 aaatctttat tgttctttaa atattttaaa taatctctca cagacatcta cccatccttt
    15661 cgtaccaaga tgcattacat cccttaaata atacttatct gaccctttat ctttaaggtt
    15721 tagaacttca aagcctttgc tttctgctat tttttgcgct ttatcgtagt actgatttct
    15781 ttctttttca gaaataccag tcaaattata gaatttatcc atacttggga ttaatacaac
    15841 aactggctta atacctaagt cagtacatac atttagcatc agtttataat tttcaaactc
    15901 cttagatgtt aataaattta catctttgtc tcttcctttg tattgctcta taccatcttt
    15961 aaaatgctct ttataatagt acttatcaat atttagtgga tttttaccaa ctcttttttt
    16021 agcatcttca atggctttct ttctctcata tgaccaattt attggtcttt ttctttttgt
    16081 tgctctcttt ttatctagct taattaatcg tttataaaga ataccctttt ctttaagagc
    16141 tatacaatac tttcttcctt gaaaataagg ctctaaaagc accttttcag ctttttcagg
    16201 aaatgtctta ggctcatata gcttagcata taatgcttct gctttatact cgtcacttcc
    16261 ccataataac ttacttgatt tttttgcata ttcaatctta ttttgtttag atatttttgg
    16321 attatctaaa aatcgataaa attgtattgg tgaaaatcta gactgataat ggtgacttgt
    16381 gactccgtct ttttccataa accattgcat agaaattaac aagacaactt tcttattatc
    16441 aatattagga ttcatacttc ctaatattgc agcatcttgt aatgtctgag tataggctct
    16501 cccaattgca aacaccttat ttttactcct atttgtatta aaatagtact ttggatgttg
    16561 ttttgttgaa ttactaagtt ctgatgaccc caaaatcata atatcctttt ctcttaaaaa
    16621 atgattattt gctgtaaccc ctttatcttt tatatcactt aaagtatcat ccataattgg
    16681 aagtagattt ttctccctca aaagttcatc agttttagaa tcaagaaatt tgtctaaccc
    16741 aaataaaaat actacgccta ttataaaagg tgtaataaag tatatcaatt ttctcatatt
    16801 ttctctcctc taaaaatatt caaatttatg ttattattaa tcatattagt tttgatatgt
    16861 atattcaact acaaaattgt aataaaatcg tcaaaacttt ttttgatact gttacaaaat
    16921 tgtaaaaaaa aagaaaaaaa cgccaaaaat aaatttcttt ttagcacttt ttcatactta
    16981 taatttgaca ttttaacact tggttaagta tacaccttac cttaacttaa aattaaattt
    17041 atttaagaat atatattata aattcttata ttttctaata taaactatat actatcagct
    17101 ggataaacaa tactactttg tcttcttatc tcatccataa tttccattac tattattgaa
    17161 ttttcatgag aatttatatt tgattctatt ttattgttat ttataagact tataaactct
    17221 acaagttcat aatacattcc ttctatttct ctaggctttt cctctgcttt ttcttttgga
    17281 atagatatat cttcttctct tccatctctg tatttaataa tagctttttc aaatagatta
    17341 agcttttcaa ttattatact tccctcttct ccttgtattt cagatggtat ataagaatca
    17401 gctactttag aatactgaat tatcgcatcc atatcatcat attgaaatac agctgaacct
    17461 tctccatcca ctccagtttg aagtatatga gaaactgcct ttacagtttt tggagcacca
    17521 aataaattca ccataggatg tatacaatac actcctatat ccattaaagc tccatttgac
    17581 aactcttttt taaaggcatt ttctataatt ccatttttat atttatcata tcttgaagaa
    17641 tactgacaat agcttccaaa aaatcttctt attttcccta ttttatataa gttttcttta
    17701 actgctttaa agtttggaac acatgtaatt ctcatagctt ccataagcaa tacattatta
    17761 tctttggctg tttttatcat tgatttgact tcttttaaat tagatgctaa cgacttttca
    17821 caaagcacat gttttttgtg ttttaaacaa gtaatggttt gctttgaatg taatgcattt
    17881 ggagaagcta tgtatacagc atctatatcc ttcgatttag ccatttgttc taaatcatca
    17941 aaaaaaatac ttgctccata tagagttcca aattctctag ctttttctaa atttctagaa
    18001 tatacagcaa ccaattcaaa gtctttaacc ctagatgcag ctcttagaaa tatatttgct
    18061 atattgcttg tcccaacaat accaaatctt attttaccca tcataaatca ctccccattt
    18121 tctacacttt attttaattt taataaaaaa ctttacaact tatagatata ttataaaata
    18181 attttatcaa ataaaaaaga aagctaattt taaatgattt caccatttta acatagcttt
    18241 cttttaacta caatctaaat tcaacttata tttttaaagt tatctttcta ctatatcttt
    18301 atttcccctc aaaaatttta tagtaagaat ttcatccaca aacaatgtta ctaaaagtat
    18361 agttacaaca attggccaat aaggtattat aagactatat aaactatcaa ctatatacac
    18421 ttgaaaatat ctaactccaa taaatgtcaa tatagtccaa tatacagaaa actttagaca
    18481 tactattcct ctactattgt acttataatc tgaataatcc caatagacct tattaaaata
    18541 tactttcata agtattcctg taatatattc tactactgtc ggcactacta gacataataa
    18601 aagcaatgca aatatattat tggggtcaat aaatcttgag acctctatta taatcgacat
    18661 tgcaattgca tacattggtt taaatggacc atttaaaaat ccatcttctt gaaaatgccc
    18721 ttgtttataa taactatata agttttcaat tatccaacct ataaaaccat acaagaaaaa
    18781 attaaataaa aaatataata gagaacctac attatccatg aatattcctc ctagctgttt
    18841 atacttattt tttctcatat tcatggattt attctaaatc aaagaaatta tatttaattt
    18901 atttaattaa tactttatat tatagattgt gacattcttt agagttcaaa ctttttaggt
    18961 attctctaac ttccagtaaa ttttcaagta tcttatgtga ttgtgattta atttgctcat
    19021 ctggctcttt taaatctggt acatttatac atcttatacc accattgtat gcagcttcaa
    19081 ctcccatagg cgaatcctct atgacaatac aatttttggg attaacattt atctttttag
    19141 ctgcttttaa gaatatctca gggtttggtt tagagtttac aacatcatct ccacaaacta
    19201 tagcatcaaa gtaatctttt aaatttgcct tagctaatct ctttactgct ctttctctct
    19261 ttgttgatgt tgcaacagcc attttgtatc cattttcctt taaaaaagag attagttcat
    19321 tgactcctaa tttgatagga gctcccttcc tctccataaa ttcaatcata tttttagttt
    19381 tttcgtcata taaatctatt attggtacag aactatcgta tatatctgta agaccttcta
    19441 ttattccttt acgatttctt cccataactg atgtataaat ttccttagtc atagtatatc
    19501 catacttttc aaacgtctcc atccaaaatt caagagaaat tctctctgag tcaaacaaaa
    19561 ctccatccat gtcaaaaata attccttcta cattttgcat acaattcctc caatattttt
    19621 tgatataatt atattacact ttttatgatt aattttacaa ccctcaattt atccaattct
    19681 aatattgtaa aattgtaatt ttcaaaagtt acactttggt ttactttaat atttgaacca
    19741 agcatatagg atatccatcc acctatactg tcaaatccat catgctctat atcaagtcca
    19801 aaatacttat taatatagtt gatagattca gttccatcaa ctaagtaaga acctgctcct
    19861 attttcttaa tatgagtctc ttctttgtca aattcatctt gaatttctcc aactatctct
    19921 tctagtatat cttctattgt taccacacca gatgtaccac catattcatc cacaactata
    19981 gcaagttgaa gtttttcctt tcttattctc tcaagagctt tatctatagt taaattttca
    20041 gatatatata taatatctct caatatttct tctagttcaa ttttattttc atttatcttt
    20101 tgattgtata aatctcttat atgtacaaaa cctaatatat catctttatt ttttctgcat
    20161 actggatatc ttgtaacacc ttcctctttt aaaatagcta atatcttttc ttcagaatca
    20221 ctttcatata tacataccat atcagttcta ggaaccatta tctctctaat tttcttttct
    20281 tcaaattcaa aaatattatc aactaatctt tgctctgatt catctataag tccactttta
    20341 taactttctt ctactaatag tcttatttcg tcacctgtat ggggttcatc aacctcatca
    20401 gcttgtgaat atccaaatgg tttaagaaat aaatcagtac ttaagttgaa aaaatatatt
    20461 attggatatg ttaacttata aaatcctact aataaaaaag atgtactcaa cattattttt
    20521 tctgtattat aaagtgctaa agctttagga accaactctc ctattacaac ctccatcatt
    20581 gttataatta aaaaagaaat ggaaattgat atagtatata taatactttc actaagatta
    20641 aatacactta ctattggtaa aattaactct ttaactgtag attctcccat ccaacctaat
    20701 gcaagtgaac aaagtgttat accaagttga catgcagaaa gataggaatt taagttgtct
    20761 tttattattt tacatctttt tgcattatta ttaccttcag ttatcatggt atcaatccta
    20821 gaactacgaa gttttaccat agcaaattca caagctacaa aaaaagcatt tatcaaaaca
    20881 agcattacta taagtgttac ttttaataac tcccgcataa agtattggtt cttataatgt
    20941 aagaaatcta taagaactca aagttccccc cttatatt
//
LOCUS       DAELLV010000043        20757 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.043, whole genome shotgun sequence.
ACCESSION   DAELLV010000043 DAELLV010000000
VERSION     DAELLV010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20757
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.043"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            370..681
                     /gene="rpsJ"
                     /locus_tag="KPD58_001330"
     CDS             370..681
                     /gene="rpsJ"
                     /locus_tag="KPD58_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBG0265109.1"
                     /translation="MAKNEKIRIRLKSYDHKLLDFSAGKIVETAKKAGSQVSGPVPLP
                     TEKQVVTILRAVHKYKDSREQFEIRTHKRLIDIANPTPKTVDSLMRLDLPAGVDIEIK
                     L"
     gene            773..1402
                     /gene="rplC"
                     /locus_tag="KPD58_001331"
     CDS             773..1402
                     /gene="rplC"
                     /locus_tag="KPD58_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBG0265110.1"
                     /translation="MKGILGKKVGMTQIFTDKGVVIPVTAVEAGPMVVTQIKTVDKDG
                     YNAIQIGFEDAKEKALNKPKKGHLAAANVLKKHLKEFRVDSVEGYTVGQEIKADVFEA
                     GAKIDVTGISKGKGFQGPIKRHGQSRGPETHGSRYHRRPGSMGACSYPGRVFKNKKLA
                     GHMGSVKVTVQNLEVVKVDADKNLILVKGAIPGAKGSVVTIKEAIKVSK"
     gene            1432..2055
                     /gene="rplD"
                     /locus_tag="KPD58_001332"
     CDS             1432..2055
                     /gene="rplD"
                     /locus_tag="KPD58_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBG0265111.1"
                     /translation="MPKLNVLNVSGQNVGEIELSDSIFGVEVNGHVLYEVVKNQLANK
                     RQGTQSAKTRAEVRGGGRKPWKQKGTGRARQGSTRSVQWVGGGVAFAPKPRSYKYTLP
                     KKVRRLAMKSALSSKVQNSEVIVLDALNMDAPKTKEFAQILNNINAAKKALVVIADKN
                     DNVIKSARNIEGVQTALVNTMNVYDILKYDSFIITTDAVKKVEEVYA"
     gene            2055..2345
                     /gene="rplW"
                     /locus_tag="KPD58_001333"
     CDS             2055..2345
                     /gene="rplW"
                     /locus_tag="KPD58_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBG0265112.1"
                     /translation="MTNPHDVIIRPVVTEHSMAEMGEKKYTFVVAKDANKTEIKKAVE
                     KVFGVSVDKVNTLNYDGKVKRMGRTQGRTSSFKKAVVKLTADSKEIEFFQGM"
     gene            2375..3205
                     /gene="rplB"
                     /locus_tag="KPD58_001334"
     CDS             2375..3205
                     /gene="rplB"
                     /locus_tag="KPD58_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBG0265113.1"
                     /translation="MAIKKFRPTSPALRQMTVLVSDEITCNQPEKSLLVNLKKNAGRN
                     VHGRITVRHRGGGQKRKYRIIDFKRDKDGIPAKVATIEYDPNRTANIALLNYADGEKR
                     YILAPVGINVGDTILSGLGADIKPGNCLALKDMPVGTIIHNIELKPGKGAQLVRSAGV
                     SAQLMAKEGKNALLRLPSGEMRLVSINCKATIGQVGNIEHGNVVIGKAGRKRHMGIRP
                     TVRGSVMNPNDHPHGGGEGRSPIGRPSPVTPWGKPALGYKTRKKNKASNKLIVSRRTK
                     "
     gene            3240..3521
                     /gene="rpsS"
                     /locus_tag="KPD58_001335"
     CDS             3240..3521
                     /gene="rpsS"
                     /locus_tag="KPD58_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBG0265114.1"
                     /translation="MSRSTKKGPFVHARLLKKIEAMNASGNKEVIKTWSRSSTVFPQM
                     VENTIAVHDGRKHVPVYITEDMVGHKLGEFVPTRTFKGHKDDEKSNKRK"
     gene            3553..3888
                     /gene="rplV"
                     /locus_tag="KPD58_001336"
     CDS             3553..3888
                     /gene="rplV"
                     /locus_tag="KPD58_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBG0265115.1"
                     /translation="MEAKATAKYVRVSPRKAGQICDLVRGKNVDEALAILKFTPRGAA
                     SIIAKVVKSAKANAENNHEMDTEKLYIASIVANQGPTMKRFMPRAMGRATTIRKRTSH
                     IEVVVKEKK"
     gene            3911..4726
                     /gene="rpsC"
                     /locus_tag="KPD58_001337"
     CDS             3911..4726
                     /gene="rpsC"
                     /locus_tag="KPD58_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBG0265116.1"
                     /translation="MGQKVNPHGLRVGVIKDWDSRWFATDKKEFGNLLLEDHNIRKFL
                     KKRLYSAGVAKIEIERSANKIKMDLHVAKPGVVIGRAGAGIEALKAELEKMTKKTIIV
                     NIVEVRSTDKNAQLVAENIALAIERRVAFRRAMKQAIQRAMKSGAKGIKVSASGRLGG
                     AEMARTEGYSEGNVPLQTLRADIDYGFAEADTTYGKIGIKVWICNGEVLPTRDGVNPR
                     EESRKSDRRDNKRDNRRNDRRGNDRRGNDNRGNYRGQRPQGGSRPQRTENKGN"
     gene            4762..5193
                     /gene="rplP"
                     /locus_tag="KPD58_001338"
     CDS             4762..5193
                     /gene="rplP"
                     /locus_tag="KPD58_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBG0265117.1"
                     /translation="MLMPKRVKRRRVHRGSMAGQAHKGNKVTYGEFGLVALEASWITS
                     NQIEAARIAMTRYIKRGGKVWIKIFPHKPVTRKPAETRMGAGKGSPEYWVAVVKPGRV
                     MFELAGVSEDKAREAMRLAAHKLPIKCKFVKKEDLEVKGGE"
     gene            5195..5398
                     /gene="rpmC"
                     /locus_tag="KPD58_001339"
     CDS             5195..5398
                     /gene="rpmC"
                     /locus_tag="KPD58_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBG0265118.1"
                     /translation="MKAKELRDLTSEELMNKLNDFKSELFSLRFQLATGQLENTARIK
                     FVKKDIAKVKTVLAERKLYETRA"
     gene            5422..5676
                     /gene="rpsQ"
                     /locus_tag="KPD58_001340"
     CDS             5422..5676
                     /gene="rpsQ"
                     /locus_tag="KPD58_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBG0265119.1"
                     /translation="MERGRRKVRIGRVVSDKMDKTIVVAVEEFVRHPLYNKRVKRTKK
                     FKAHDEKNICNIGDRVKIMETRPLSKDKRFRLVEVVEKVK"
     gene            5702..6070
                     /gene="rplN"
                     /locus_tag="KPD58_001341"
     CDS             5702..6070
                     /gene="rplN"
                     /locus_tag="KPD58_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBG0265120.1"
                     /translation="MIQQESRLRVADNSGAKELLCIRVLGGSKRRYGNIGDVIVATVK
                     SATPGGVVKKGKVVKAVIVRSKQGVRRNDGSYISFDENAAVIIKDDKTPVGTRIFGPV
                     ARELRDNEFMKIVSLAPEVL"
     gene            6094..6399
                     /gene="rplX"
                     /locus_tag="KPD58_001342"
     CDS             6094..6399
                     /gene="rplX"
                     /locus_tag="KPD58_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBG0265121.1"
                     /translation="MRVKKGDTVVVIAGKDKGKKGSVLKVYPKTSKVLVEGVNVITKH
                     QKPSAMNQQGGIINKEAPIHISNVMPFDPETGKGVRVRYEVKDGNKVRVSAKSGKEL"
     gene            6429..6971
                     /gene="rplE"
                     /locus_tag="KPD58_001343"
     CDS             6429..6971
                     /gene="rplE"
                     /locus_tag="KPD58_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBG0265122.1"
                     /translation="MASRLQEKYMKEVAPALMEKFGYKNVMEIPKLNKIVINMGIGDA
                     RENPKGLEKGVEELEMISGQKPVITKARKSVANFKLREGMPIGTKVTLRADKMFYFMD
                     KLVSVSLPRVRDFRGVNPNAFDGRGNYALGVKEQLIFPEIEYDKIDKVRGMDIIFVTT
                     AKTDEEARELLKLLGMPFSK"
     gene            6989..7174
                     /locus_tag="KPD58_001344"
     CDS             6989..7174
                     /locus_tag="KPD58_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HBG0265123.1"
                     /translation="MARKAMVVKQQRKQKYATREYTRCTICGRPHSVLKKFGICRICF
                     RELAYKGQIPGVRKASW"
     gene            7207..7605
                     /gene="rpsH"
                     /locus_tag="KPD58_001345"
     CDS             7207..7605
                     /gene="rpsH"
                     /locus_tag="KPD58_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBG0265124.1"
                     /translation="MTMTDPIADMLTRIRNANVVKHETVDVPASNMKKELARILLEEG
                     FIRGYDVIEDGKQGIIRIQLKYGQEGERVITGLKKISKPGMRVYAANHEIPKVLNGLG
                     ISVISTSKGILTDKQARKENVGGEVICYVW"
     gene            7636..8178
                     /gene="rplF"
                     /locus_tag="KPD58_001346"
     CDS             7636..8178
                     /gene="rplF"
                     /locus_tag="KPD58_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBG0265125.1"
                     /translation="MSRIGVKPIIIPAGVEVTIAEGNLVTVKGPKGTLTKQLSAELNI
                     KKEENTIMVERPTDNKKHRSLHGLTRTLLDNMVVGVNTGFEKKLELKGVGYRAQKQGK
                     KLVMNLGFSHPVEMEDPEGITVEAPNQTELIVKGIDKQLVGNYAAKIRAWRKPEPYKG
                     KGIKYVDEVIRRKEGKTGKK"
     gene            8214..8582
                     /gene="rplR"
                     /locus_tag="KPD58_001347"
     CDS             8214..8582
                     /gene="rplR"
                     /locus_tag="KPD58_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBG0265126.1"
                     /translation="MLKKADKNANRLQRHKRVRRKISGTSQRPRLCVFRSANNIYAQI
                     IDDTKRVTLVAASSLEAEVKSAVNHTGNKEAAKKVGELVAKKAVEKGITEVVFDRGGY
                     LYHGRIQELAEGAREAGLKF"
     gene            8603..9112
                     /gene="rpsE"
                     /locus_tag="KPD58_001348"
     CDS             8603..9112
                     /gene="rpsE"
                     /locus_tag="KPD58_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBG0265127.1"
                     /translation="MLRRKPIDAGQLDLQEKVVEVRRVTKVVKGGRNFRFAALVVVGD
                     ENGHVGIGAGKAMEVPDAIKKAVEDAKKNLIVVPIVGTTIPHEVRGHFGAGNILIMPA
                     VEGTGVIAGGPARAVLELAGLKDVRAKSLGSNNPRNMVNATIEGLNSLKTVEDIAKLR
                     GKKVEELLG"
     gene            9127..9312
                     /gene="rpmD"
                     /locus_tag="KPD58_001349"
     CDS             9127..9312
                     /gene="rpmD"
                     /locus_tag="KPD58_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBG0265128.1"
                     /translation="MAKLQIKLVRSVIGTTPNQKKNVEALGLRKREQVVVKEDNAQTR
                     GMINKVSHLLEVTEIAE"
     gene            9345..9788
                     /gene="rplO"
                     /locus_tag="KPD58_001350"
     CDS             9345..9788
                     /gene="rplO"
                     /locus_tag="KPD58_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBG0265129.1"
                     /translation="MKLHELKPAEGAVRAKRRLGRGTATGQGKTAGRGQKGQWSRSGG
                     GVRVGFEGGQMPLARRLPKRGFNNIFKKVYTEVNVEVLNRFENGTEITAELLKSTKTI
                     SKIGKDGIKILGEGNLEKALTVKAAKFTASAQEKIEKAGGKAELV"
     gene            9832..11100
                     /gene="secY"
                     /locus_tag="KPD58_001351"
     CDS             9832..11100
                     /gene="secY"
                     /locus_tag="KPD58_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBG0265130.1"
                     /translation="MLSKLKQAWKIKAVRKKVMYTLMMIVIFRIGTTIPVPGIDTSII
                     QKMVGGNSLLSLYNMFTGGAFSNFSLFALGISPYITASIIIQLLTVGFESLAELQKSG
                     EEGKKKINKYTKYTALALAVVQALGITLGIVRSALISNSVFFITTVVITLVSASMLVM
                     WIGDKITEKGIGNGSSVIIFAGIISRIPTDVIKISQQVKSGEVAPWVIVILAVVILLT
                     VTGVTFIQEATRKIPVQYAKRVVGRKMYGGQSSHIPMKVNQSGVMPIIFASSLLAFPQ
                     TIAMFMGPNAQAFVQKYLSMATEQGFWTYRSIEILLIIFFSYFYTTVSFNTEDISKNM
                     KNNGGFIPGIRPGEPTMDYLNRILTRLTLAGATFLAIIAMVPALTTHYMKVNMSLAGT
                     SLLIVVGVALELKRQLESNLVMRSYQGFLK"
     gene            11117..11767
                     /locus_tag="KPD58_001352"
     CDS             11117..11767
                     /locus_tag="KPD58_001352"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBG0265131.1"
                     /translation="MRIILLGPPGAGKGTQAAGIVEKYNIPHISTGDIFRKNIKEGTE
                     LGKKAKEYMDQGLLVPDELTVGLVTDRISQEDCKNGFMLDGFPRNVAQGEHLDIFLKN
                     AGISLDKVVNIEVDKSILVSRAVGRRICKSCGATYHVEFNPPKVEGVCDVCQGELYQR
                     ADDNEETVSKRIQVYLDETKPLVDYYSKQGIIADIKGDQAIDKVFEDIVAALGSGK"
     gene            11768..12514
                     /gene="map"
                     /locus_tag="KPD58_001353"
     CDS             11768..12514
                     /gene="map"
                     /locus_tag="KPD58_001353"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBG0265132.1"
                     /translation="MIILKSKKEIELLREAGKIVADTHEVLRKAISPGISTLELDKIA
                     EENIRKYNAEPSFKGYGGFPGSICASINREVVHGIPGETILQEGDIVSLDIGAYYKGY
                     HGDSAKTHGVGMISEEDRKLIEVTRESFYEGIKFAKLGYRLSDISHAVQTHVEKHGFS
                     VVRDLVGHGVGANLHEDPQVPNYGLPGKGPRLREGMVIAIEPMVNAGRYHVKTLSDGW
                     TTVTIDGKKSAHYEHTIAITEHEPLILTKL"
     gene            12536..12805
                     /locus_tag="KPD58_001354"
     CDS             12536..12805
                     /locus_tag="KPD58_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing protein"
                     /protein_id="HBG0265133.1"
                     /translation="MLSDNLSIGQVVKVSLGRDKGNLFFVVKIINNEYVLIADGKKRK
                     LDKPKLKKVKHLKKYNFINDEVRKRVVSGQEITDSFLRAELTKLN"
     gene            12843..13061
                     /gene="infA"
                     /locus_tag="KPD58_001355"
     CDS             12843..13061
                     /gene="infA"
                     /locus_tag="KPD58_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBG0265134.1"
                     /translation="MAKKDVIELEGTVSEALPNAMFKVKLENGHEILCHISGKLRMNF
                     IRILEGDKVNVELSPYDLTRGRITWRKK"
     gene            13097..13210
                     /gene="rpmJ"
                     /locus_tag="KPD58_001356"
     CDS             13097..13210
                     /gene="rpmJ"
                     /locus_tag="KPD58_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBG0265135.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQKQG"
     gene            13363..13734
                     /gene="rpsM"
                     /locus_tag="KPD58_001357"
     CDS             13363..13734
                     /gene="rpsM"
                     /locus_tag="KPD58_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBG0265136.1"
                     /translation="MARIAGVDLPREKRAEIGLTYIYGIGKATANEILAKAEINPDTR
                     IKDLSEDQVNELRKVIDDDFLVEGDLRREIALNIKRLRDIKCYRGIRHAKGLPLRGQR
                     TKTNARTRKGPRKTVSRKKKK"
     gene            13770..14168
                     /gene="rpsK"
                     /locus_tag="KPD58_001358"
     CDS             13770..14168
                     /gene="rpsK"
                     /locus_tag="KPD58_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBG0265137.1"
                     /translation="MAKPKKKVTRIRRRERKNIERGHAHIQSTFNNTIITLTDVHGNA
                     ISWASSGQLGFKGSRKSTPFASQMAAETAAKAAMEHGLKSVEVFVKGPGSGREAAIRA
                     LQATGLEVTMIKDVTPIPHNGCRPPKRRRV"
     gene            14200..14823
                     /gene="rpsD"
                     /locus_tag="KPD58_001359"
     CDS             14200..14823
                     /gene="rpsD"
                     /locus_tag="KPD58_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBG0265138.1"
                     /translation="MARYTGASCRQCRREGMKLFLKGDRCYTDKCAIVKRNYAPGQHG
                     QGRKKVSNYGLQLREKQKVKRIYGVLETQFRNLYERAENMPGKAGENLLSLLERRLDN
                     VVYRMGLASSRKEARQLVTHGHFTLNGNKVDIPSLIVKVGDVIEVKEKSRSSAKFKNL
                     VEVNSRIAPKWLEANVEGMTAKVVGVPTREDIDLEIAEHLIIELYSK"
     gene            14901..15848
                     /locus_tag="KPD58_001360"
     CDS             14901..15848
                     /locus_tag="KPD58_001360"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBG0265139.1"
                     /translation="MIEIEKPKVDIVELSEDYRYGKFVIEPLERGYGITIGNALRRIL
                     LSSLPGVAVNAIKIDGVLHEFSTIPGVKEDVTEIILTLKELSATIDGEGSRTLKIEAQ
                     GPCSITGADIICPPDVEILSKDLAIATLDDNAKLNMEIFVDKGRGYVSAEENKTENVP
                     IGVLPVDSIYTPVEKVSYHVENTRVGQKTDYDKLVLEVWTNGSINPQEGISLAAKVLV
                     EHLNLFIDLTEHVSSVEIMVEKEEDQKEKVLEMTIEELDLSVRSYNCLKRAGINTVEE
                     LANKSEDDMMKVRNLGKKSLEEVIQKLEELGLGLKPSEE"
     gene            15869..16210
                     /gene="rplQ"
                     /locus_tag="KPD58_001361"
     CDS             15869..16210
                     /gene="rplQ"
                     /locus_tag="KPD58_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBG0265140.1"
                     /translation="MAKYRKLGRETAHRNLMLRNLVTCLLRSGRIETTVTRAKETRRM
                     AEKMITLAKRGDLHARRQVLAYVMDETVVNNLFTDLAPKYAERNGGYTRIIKIGPRKG
                     DAAEMAFIELV"
     gene            16330..17163
                     /locus_tag="KPD58_001362"
     CDS             16330..17163
                     /locus_tag="KPD58_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HBG0265141.1"
                     /translation="MDNIVKVNNISFEYITDEAKLKAIDNLSLDVKKGEFVAIIGHNG
                     SGKSTLSKNLNAILMPTEGNILIDDMDTKEEERLWDIRQTAGMVFQNPDNQIVATIVE
                     EDVAFGPENLGIEPKEIRRIVEESLKSVGMYDLRDRQPHLLSGGQKQRVAIAGIIAMR
                     PKCIIFDEATAMLDPSGRKEVMKTIKRLNKEENITVIHITHFMEEAVEADRVVVMEKG
                     KKILEGTPREVFSKIKMLKEIGLDVPCMTELSSLLIEEGINISSDILTVDEMVMELCQ
                     L"
     gene            17151..18017
                     /locus_tag="KPD58_001363"
     CDS             17151..18017
                     /locus_tag="KPD58_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HBG0265142.1"
                     /translation="MSIIVKNLTHIYNEGMPFASKALDDVSFEIKDRDFVGLIGHTGS
                     GKSTLIQHLNGLLKPSSGEIFINDFNITDKNLNLTEIRKRVGVVFQYPEYQLFEETID
                     KDIAFGPSNLGLEESEIHNRVKASMEAVGLDYEGFKDKSPFELSGGQKRRVAIAGVIA
                     MNPEVLILDEPTAGLDPGGRDEIFNLIKDLHEKKNMTIILSSHSMDDMAKLAKTLIVM
                     NHGSVEFMGTPREVFKSNASKLKDIGLDIPQVLELALKLREKGFDISEDILTLEEAKQ
                     EILKVVRGRGLC"
     gene            18011..18814
                     /locus_tag="KPD58_001364"
     CDS             18011..18814
                     /locus_tag="KPD58_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBG0265143.1"
                     /translation="MLKDITIGQYYPTSSAIHKLDPRIKLVATIVFMVSIFVVNKFWP
                     YIVVLLCLLAMIKLANIPVKYIVKGVKPLKWIILFTFLINIFFLPGDEIWSFGFLAIT
                     KQGLRQAIFMAIRLIFLVVGTSLLTLTTSPIELTDGIERLLNPFKKIGLPVHELAMMM
                     TIALRFIPTLLDETDKIMKAQMSRGADFESKNLINRAKNLVPLLVPLFVSAFRRADEL
                     AMAMEARCYRGGHNRTKMRESVISRRDYMACVFQVVYLGAIIATRFIAI"
     gene            18846..19577
                     /gene="truA"
                     /locus_tag="KPD58_001365"
     CDS             18846..19577
                     /gene="truA"
                     /locus_tag="KPD58_001365"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBG0265144.1"
                     /translation="MRNIKIKIQYNGKNYCGWQKQPDSLGIQGTIERAIYDITKEETS
                     LIGSGRTDSGVHAIGQIANFKINSGISIESIPMALNAKLPKDISVIEACEVNDDFHSR
                     YSAKGKTYKYLVYNSKFRNPILSEISYQVKYELDFDKMCSEAKSLLGTHDFKGFMSSG
                     SSVKDTVRTIYDIDISKKDDLITFEISGNGFLYNMVRIIVGTLVDMGRGRINEPFLDI
                     IQSKTRSRCGHTAPAQGLFLKKVHY"
     gene            19697..20128
                     /gene="rplM"
                     /locus_tag="KPD58_001366"
     CDS             19697..20128
                     /gene="rplM"
                     /locus_tag="KPD58_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBG0265145.1"
                     /translation="MKSYIAKPADVQRKWYLVDAEGKTLGRLATEIATVLRGKHKPTF
                     TPHVDGGDFVIVVNAEKIVLSGKKLDQKYYRYHTGYVGGLKEISYRDMMDKKPEEVIS
                     HAVSGMLPKNKLRSRMMTRLRVFAGAEHTHAAQNPEVLNFK"
     gene            20157..20549
                     /gene="rpsI"
                     /locus_tag="KPD58_001367"
     CDS             20157..20549
                     /gene="rpsI"
                     /locus_tag="KPD58_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBG0265146.1"
                     /translation="MANVQYYGTGRRKSSVARVRLVAGEGNILVNGRALENYFNYETL
                     IRDVKQPLVLTGNENKYDVIVKVEGGGFTGQAGAIRHGISRALLKADLDLRPALKKEG
                     FLTRDARMKERKKYGLKAARRAPQFSKR"
BASE COUNT         8291 a         2451 c         4320 g         5695 t
ORIGIN      
        1 ctttactaaa ctaatggatt taatataaaa aaagaggggg gtaactcctc tcttttttgt
       61 tttttgttga caagtagcgc taaagcatat atactaaact agtgccttga gacagattaa
      121 caatcaagtt taaggttatg atgttgtaat agaaaataaa attgaaaaaa acagaaaaaa
      181 aacttgaaaa aatgtaacag atgatataaa atataatagt gtgcttgaga gattaagaaa
      241 ataataatta aatcctagtg aaatacgaac tgatatcgat ttaaaagtta caacacaccc
      301 ggagcagtgt atcggtattg gttgtacgca acttgaaaat taaacacgtg ataaaggagg
      361 gaaaacaaaa tggctaagaa tgaaaaaata agaataagat taaaatcata tgatcacaaa
      421 ttattagatt tttcagcagg taaaatagtt gaaactgcta aaaaggctgg atcacaagtt
      481 tcaggacctg tgcctctacc aacagaaaaa caagttgtaa ctatattaag agctgtacat
      541 aagtacaaag actctagaga acaatttgaa ataagaactc ataaaagact aatcgacata
      601 gctaatccaa cacctaagac agttgactca ttaatgagat tagacttacc agcaggtgta
      661 gatatagaaa taaaattata agaaccccta agatattatc ctatagatga ttatcttaga
      721 atgattactc taaaagatgt aatccgctgt aagaattata ggaggtgcta atatgaaagg
      781 aatattaggc aaaaaagttg gtatgactca aatattcact gataaaggtg tagttatacc
      841 tgtaacagct gttgaagcag ggcctatggt cgttactcaa ataaaaacag ttgataaaga
      901 tggatacaat gctatacaaa taggttttga agatgctaaa gaaaaagctt taaacaaacc
      961 taaaaaagga catttagctg ctgctaatgt tttaaagaaa catttaaaag aatttagagt
     1021 agattctgta gaaggataca cagtaggaca agaaataaaa gctgatgtat ttgaagcagg
     1081 tgcaaaaata gatgttactg gaataagtaa aggtaaggga ttccaaggtc caataaaaag
     1141 acatggacaa agtagaggtc ctgaaactca cggttctaga taccacagaa gaccaggttc
     1201 aatgggagct tgttcttacc caggtagagt atttaaaaac aaaaaattag ctggacatat
     1261 gggaagcgta aaggtaacag ttcaaaactt agaggttgtt aaagtagatg ctgacaagaa
     1321 ccttatatta gttaaaggag ctataccagg agctaaaggt tcagtagtaa ctataaaaga
     1381 agctataaag gtttctaaat aatgactaac ctagagaaag gaggaataac aatgccaaaa
     1441 ttaaatgtac taaatgttag tggacaaaac gttggagaaa tagaattatc agattctata
     1501 tttggcgtag aagttaatgg acatgtttta tacgaagttg taaaaaatca attagcaaat
     1561 aagagacaag gaactcaatc tgctaaaact agagcagaag ttagaggtgg cggaagaaaa
     1621 ccttggaaac aaaaaggaac aggtagagca agacaaggtt ctacaagatc tgtacaatgg
     1681 gtaggtggag gagttgcttt tgcacctaaa ccaagaagct acaaatatac attacctaag
     1741 aaggtaagaa gattagctat gaagagtgct ttatcttcaa aagttcaaaa tagtgaagtt
     1801 atagtattag atgcattaaa catggatgct cctaagacta aagaatttgc tcaaatatta
     1861 aacaatataa atgctgctaa aaaagcttta gtagttatag ctgacaaaaa cgataacgta
     1921 ataaaatcag ctagaaatat agaaggtgtt caaactgcat tagttaatac tatgaatgta
     1981 tatgatatat taaaatacga ttcatttata ataactacag atgctgttaa aaaagtggag
     2041 gaggtgtacg cataatgact aatccacatg atgtaataat aagaccagtt gtaactgagc
     2101 acagtatggc tgaaatgggt gaaaaaaaat acacttttgt tgtggctaaa gatgcgaaca
     2161 aaactgaaat aaaaaaagca gtagaaaaag tattcggagt tagtgttgat aaagtaaata
     2221 ctttaaacta cgatggaaaa gttaaaagaa tgggtagaac tcaaggaaga acatcaagct
     2281 ttaagaaagc agtagttaaa ttaactgctg atagtaaaga aatagaattc ttccaaggaa
     2341 tgtagtatta taacaaacga aggagggaaa acagatggct ataaaaaagt ttagaccaac
     2401 ttctcctgcc ttaagacaaa tgacagtttt agtttctgat gaaataacat gtaatcaacc
     2461 agaaaaatct cttttagtta acttaaagaa aaatgctggt agaaacgtac atggtagaat
     2521 aactgttcgt catagaggtg gaggacaaaa aagaaaatat agaataatag attttaaaag
     2581 agataaagat ggtatacctg ctaaggttgc tactatagaa tatgatccaa acagaactgc
     2641 aaatatcgca ttattaaact atgctgatgg agaaaaaaga tatatattag ctccagttgg
     2701 aattaacgtt ggagatacta tattatcagg attaggtgct gatataaaac caggaaactg
     2761 tttagcatta aaagatatgc cagttggtac tatcattcat aatatagaat taaagccagg
     2821 aaaaggagct caattagtta gatctgctgg agtttctgca caattaatgg ctaaagaagg
     2881 aaaaaatgct ttattaagat taccatcagg tgagatgaga ttagtaagca taaactgtaa
     2941 agctactata ggtcaagttg gaaatataga gcatggtaac gtagttatcg gtaaagcagg
     3001 tagaaaaaga catatgggaa taagacctac tgtaagaggt tctgtaatga accctaatga
     3061 ccatccacac ggtggagggg aaggtagatc tccaataggt agaccatctc ctgttactcc
     3121 atggggtaaa ccagctcttg gatacaaaac tagaaagaaa aataaagctt caaataaact
     3181 aatagtatca agaagaacta agtagtaaat ttatttttgc tcggaaagga ggaatataaa
     3241 tgtcaagatc aactaaaaaa gggccttttg tccatgcaag acttttaaag aagatagaag
     3301 ctatgaatgc tagtggaaat aaagaagtta taaaaacttg gtcaagatct tcaactgtat
     3361 ttccacaaat ggtagaaaac actatagctg ttcatgatgg aagaaaacat gttccagtat
     3421 atataacaga agatatggtt ggacataaat taggtgagtt cgttcctact agaactttca
     3481 aaggacataa ggatgacgaa aaatctaata agagaaaata attaaattat gactaagaaa
     3541 ggaggaataa caatggaagc aaaagctact gctaaatacg tacgtgtatc accaagaaaa
     3601 gcaggccaaa tatgtgacct tgttagagga aagaatgttg atgaagcatt agcaatatta
     3661 aagtttactc caagaggagc agcttcaata atagctaagg ttgtaaaatc agctaaagct
     3721 aacgcagaaa acaatcacga aatggatact gaaaaattat atatagcatc aatagttgct
     3781 aatcaaggac caacaatgaa gagattcatg cctagagcta tgggtcgtgc aactacaata
     3841 agaaaaagaa cttctcatat agaggttgtt gttaaagaaa aaaaataatt aatagatagg
     3901 agggaaagta atgggtcaaa aggttaatcc acacggctta agggtcggtg ttataaaaga
     3961 ttgggactca agatggttcg ctactgataa aaaagagttc ggaaacttat tattagaaga
     4021 ccataatata cgtaaattct taaagaaaag attatactca gctggagttg ctaagataga
     4081 aatagaaaga tcagctaaca aaataaaaat ggacttacac gttgctaagc ctggtgtagt
     4141 tataggaaga gctggtgcag gaatcgaagc attaaaagct gaattagaaa aaatgacaaa
     4201 gaaaactata atagttaata tagtagaagt aagaagtaca gataaaaatg ctcaattagt
     4261 agctgaaaat atagctttag ctatagagag aagggttgct ttcagaagag caatgaaaca
     4321 agctatacaa agagcaatga aatctggtgc taaagggata aaagtttctg cttcaggaag
     4381 attaggtgga gctgaaatgg ctagaactga aggttatagt gaaggaaatg tgccactaca
     4441 aactttaaga gcagatatag attatggttt cgctgaagct gatacaactt atggaaaaat
     4501 cggtataaaa gtttggatat gtaatggtga agttttacca actagagacg gtgtaaatcc
     4561 aagagaagaa agcagaaaga gcgatagaag agacaacaag agagataata gaagaaacga
     4621 tagaagagga aatgacagaa gaggaaatga taacagagga aactacagag gacaaagacc
     4681 tcaaggtgga tcaagacctc aaagaactga aaacaaagga aattaataat agttaggttc
     4741 aaggaaggag gaaaaatact catgttaatg ccaaaaagag taaagcgtcg tagagttcat
     4801 agaggaagca tggctgggca agctcataag ggtaataaag ttacttatgg tgagtttgga
     4861 ttagtagcat tagaagcttc ttggataact tctaatcaaa tagaagctgc cagaattgcg
     4921 atgactagat atataaaaag agggggaaag gtttggataa aaatattccc tcataaacca
     4981 gtaacaagaa aaccagcaga gactcgtatg ggtgcaggga aaggttctcc agaatattgg
     5041 gtagctgtag ttaagccagg aagagttatg tttgaattag caggtgtttc tgaagataaa
     5101 gcaagagaag ctatgagact tgctgcacat aaattaccaa tcaaatgtaa atttgttaaa
     5161 aaagaagatt tagaagtaaa gggtggtgaa taggatgaaa gctaaagaat taagagattt
     5221 aacaagcgaa gagctaatga acaaattaaa cgacttcaaa agtgaattat ttagcttaag
     5281 attccaatta gctactggtc aattagaaaa tacagctaga ataaagtttg ttaagaagga
     5341 tatagcaaaa gttaaaactg ttcttgctga aagaaagtta tacgaaacta gagcttaatt
     5401 tggaaaggag gctttttaaa catggaaaga ggaagaagaa aagttagaat aggccgtgtt
     5461 gttagtgata agatggataa aacaatagta gtagctgttg aagaattcgt acgtcatcca
     5521 ctatataata aacgtgttaa gagaactaag aaattcaagg ctcatgatga aaagaatata
     5581 tgtaacatcg gagatagagt taaaataatg gaaactagac cattatctaa agataagaga
     5641 ttcagactag ttgaagttgt tgagaaagtt aagtagtttt tgaagaagga gggattacga
     5701 tatgatacaa caagaatcac gtctaagagt tgctgataac tcaggagcta aagaactttt
     5761 atgtatacgt gttctaggcg gaagtaaaag aagatatggt aacataggcg acgttatagt
     5821 tgctactgtt aaaagtgcaa cacctggtgg agttgtaaaa aaaggtaagg ttgttaaagc
     5881 tgttatagta agaagcaaac aaggcgtaag acgtaatgac ggaagctata tatcttttga
     5941 tgaaaatgct gctgttataa taaaggacga taaaactcca gtaggaactc gtatattcgg
     6001 gcctgttgct agagagttaa gagacaatga attcatgaaa atagtttctc ttgctccaga
     6061 agtactataa ttaaggaggt gcaataggac atgatgcgtg ttaaaaaagg tgacactgtt
     6121 gtagttatag caggtaaaga taaaggtaaa aaaggttcag ttttaaaggt atatcctaaa
     6181 actagcaaag tgttagttga aggtgtaaat gtaataacta aacatcaaaa accaagtgct
     6241 atgaaccaac aaggtggaat aataaataaa gaagccccaa tacacatatc taatgtaatg
     6301 ccatttgatc ctgaaacagg aaaaggcgtt agagtaagat atgaagtaaa agatgggaac
     6361 aaagtaagag tatcagcaaa gagcggaaaa gaattataat aaatattgct tgaaaggagg
     6421 gaccttaaat ggcttctaga ttacaagaaa aatacatgaa agaagttgct cctgctttaa
     6481 tggagaaatt tggatacaaa aacgtaatgg agatacctaa gttaaataaa atagttatta
     6541 acatgggtat aggtgacgct agagaaaatc caaaaggatt agaaaaaggc gttgaagaat
     6601 tagaaatgat atcaggacaa aagcctgtta taactaaagc tagaaaatct gttgctaact
     6661 tcaaattaag agaaggaatg ccaataggaa caaaagttac attaagagct gacaaaatgt
     6721 tctactttat ggacaaatta gtttcagttt ctttaccaag agttagagac tttagaggag
     6781 ttaaccctaa tgcttttgat ggtagaggaa attatgcttt aggagttaaa gaacaattaa
     6841 tattccctga aatagaatat gataaaatag ataaagtaag aggaatggat ataatatttg
     6901 ttactacagc aaaaactgac gaagaagctc gtgaattatt aaaattatta ggaatgccat
     6961 tttctaagta agcaaaggag ggattcccgt ggctagaaaa gcgatggttg ttaagcaaca
     7021 aagaaagcaa aagtacgcta ctagagaata cactagatgt actatatgcg gaagaccgca
     7081 ttcagttttg aaaaaatttg gtatatgccg tatatgcttt agagaattag cttataaagg
     7141 tcaaatacct ggtgttagaa aagcaagctg gtagaagctt gattagaatg gaaggagggc
     7201 aacaaaatga caatgacaga tccaattgca gatatgctta ctcgtataag aaatgctaat
     7261 gttgttaagc atgaaactgt agatgttcca gcttctaata tgaagaaaga attagctaga
     7321 atcttattag aagaaggttt cataagaggt tatgatgtta tagaagatgg aaaacaaggt
     7381 atcataagaa tacaattaaa gtacggacaa gaaggcgaaa gagttataac aggtcttaag
     7441 aaaatatcta agcctggaat gagagtttac gctgctaacc atgagatacc aaaagtatta
     7501 aacggattag gaatatcagt tatatctact tcaaaaggaa tattaactga caagcaagct
     7561 agaaaagaaa atgttggtgg agaagtaatc tgctacgttt ggtaattaat atgaacaatg
     7621 taaggaggtg cgaccatgtc aagaataggt gttaagccaa taataatacc agcaggtgtt
     7681 gaagttacta tagctgaagg aaatttagtt acagtaaaag gtccaaaagg aactttaact
     7741 aaacaattaa gtgctgagtt aaacataaaa aaagaagaaa atacaataat ggttgaaaga
     7801 ccaactgata ataaaaaaca tagatcttta cacggactaa ctagaacttt attagataat
     7861 atggttgtag gtgtaaatac tggtttcgaa aagaaattag aattaaaagg tgttggatat
     7921 agagctcaaa aacaaggaaa gaaattagtt atgaacttag gtttctctca tccagttgag
     7981 atggaagatc cagaaggaat aactgttgaa gctccaaacc aaacagagtt aatagtaaaa
     8041 ggtatagaca agcaattagt agggaattat gctgctaaga taagagcttg gagaaagcca
     8101 gagccataca aaggtaaagg tataaaatac gttgatgaag taatcagacg taaagaagga
     8161 aaaactggta aaaaataata ccaaaactta aacgctagaa aggagtgagc taggtgttaa
     8221 aaaaggctga taaaaatgct aatagacttc aaagacataa gagagttcgt agaaaaataa
     8281 gcggtacttc tcaaagacca agattatgtg tatttagaag tgctaacaat atatatgctc
     8341 aaataataga tgacacaaaa agagtaactc tagttgctgc atcttcttta gaagcagaag
     8401 ttaagagtgc tgtcaatcat acaggaaaca aggaagcagc taagaaagtt ggagaattag
     8461 ttgctaaaaa agctgttgaa aaaggaatca ctgaagttgt atttgacaga ggtggatact
     8521 tatatcatgg aagaattcaa gaattagctg aaggtgctag agaagctggt cttaagttct
     8581 aatacgaagg agggaaaaat acatgctacg tcgtaagccg atagatgcag gacaacttga
     8641 cttacaagaa aaagttgttg aagtaagacg tgttactaag gttgtaaaag gtggtagaaa
     8701 tttcagattt gcagcattag tagttgttgg agatgaaaac ggacacgttg gtataggtgc
     8761 tgggaaagca atggaagtac cagacgctat aaaaaaagca gttgaagatg ctaagaaaaa
     8821 tctaatagta gtgccaatag ttgggactac aattcctcac gaagtacgcg gacactttgg
     8881 tgctggaaat atattaataa tgcctgctgt agaaggtaca ggagtaatag ctgggggacc
     8941 tgctagagcc gtacttgagc tagctggact aaaagacgtt agagctaaat ctttagggtc
     9001 taacaatcca agaaatatgg taaatgctac aatagaagga cttaattctc taaaaacagt
     9061 tgaagatata gctaaactta gaggtaaaaa agttgaagaa cttctagggt aaggaggtag
     9121 tgtaaaatgg ctaaattaca aataaagtta gttagaagtg ttataggaac tactcctaac
     9181 caaaaaaaga atgttgaagc gttaggatta agaaaaagag aacaagttgt tgtaaaagaa
     9241 gacaatgctc aaacaagagg tatgataaat aaagtaagtc acttattaga agtaactgaa
     9301 atagctgaat aatttttata aagtagtgca aggaggtgca atccatgaag ttacatgagt
     9361 taaaacctgc tgaaggtgca gtaagagcta agagaagatt aggtagaggt actgcaactg
     9421 gacaaggtaa aactgcagga cgtggacaaa aaggtcaatg gtctcgttct ggtggtggag
     9481 taagagtagg atttgaaggt ggacaaatgc cactagctag aagacttcct aagagaggtt
     9541 tcaataacat atttaagaaa gtttacacag aagttaatgt tgaagtttta aacagatttg
     9601 aaaatggaac agaaataact gcagaattat taaaatctac taaaactata agcaaaatag
     9661 gtaaagacgg aattaaaatc ttaggtgaag gtaatttaga gaaagcttta actgttaaag
     9721 ctgctaaatt tacagcttca gctcaagaaa aaatagaaaa agctggtgga aaagcagagt
     9781 tagtataata acctgctaat ctcctagcca gtacaggcgg ggtgaattag cgtgctgtca
     9841 aaattaaaac aagcttggaa gattaaagca gtaagaaaaa aagtaatgta tacacttatg
     9901 atgatcgtga tattcagaat aggtactact ataccagttc caggcataga cactagtatt
     9961 atacaaaaga tggtaggcgg caatagtcta ctttctctgt ataatatgtt tactggtgga
    10021 gcatttagta acttttcact atttgcttta ggtataagtc catatatcac tgcatcaatc
    10081 ataatacaac ttctaacagt aggttttgaa agtttagcag aacttcaaaa atctggtgaa
    10141 gaaggaaaga aaaaaatcaa taagtacact aaatatacag cactagctct agcagttgta
    10201 caagctttgg gaattacatt aggaattgta agaagtgctt taatatcaaa tagtgtgttt
    10261 tttatcacaa ctgttgtaat aacattagtt tcagcaagta tgttagtaat gtggattggg
    10321 gacaaaatta ctgaaaaagg aatcggtaat ggtagttcag taataatatt tgcaggtata
    10381 atatcaagaa tacctacaga tgttattaaa atatcgcaac aagttaaatc tggcgaagtt
    10441 gcaccttggg ttatagtgat tctagctgta gttatattac ttacagtaac aggagttaca
    10501 tttatacaag aagcaactag aaagatacct gtacaatatg ctaaaagagt tgttggaaga
    10561 aaaatgtatg gaggacaaag ttctcatata ccaatgaaag ttaaccagtc aggagttatg
    10621 cctataattt ttgctagttc acttttagct ttccctcaaa caatagctat gtttatgggt
    10681 ccaaatgctc aggcttttgt tcaaaaatac ttaagtatgg caacagaaca aggattttgg
    10741 acatatagat caattgagat tctacttata attttcttct catattttta tacaactgta
    10801 tcatttaata cagaagacat atctaaaaat atgaaaaata atggtggatt tataccaggt
    10861 ataagaccag gtgagcctac tatggattat ttaaatagaa ttttaactag attaacttta
    10921 gctggagcta ctttcttagc aataattgct atggtgccag ctcttacaac acattatatg
    10981 aaagtaaata tgagtctagc tgggacttca ttgctaatcg ttgttggagt tgctttggag
    11041 cttaagagac agttagagtc aaacttagtt atgagaagct atcaaggttt cttaaaataa
    11101 aatggagatg gataatatga gaataatatt acttggacct ccaggtgctg gtaaaggtac
    11161 tcaggcggca ggaatagtag aaaaatacaa tatacctcat atatcaacag gagatatatt
    11221 cagaaagaat ataaaagagg gaacagaact tggaaaaaaa gctaaagaat acatggacca
    11281 aggtttatta gtaccagatg agttaactgt aggtttagtt actgatagaa tatctcaaga
    11341 agattgtaaa aatggattta tgttagatgg atttccaaga aatgtagcac aaggagaaca
    11401 tttagatatc ttcttaaaaa atgctggtat atcactagat aaagttgtca atattgaagt
    11461 tgataagagt atattagtgt ctagagcagt tggtagaaga atatgtaagt cttgtggagc
    11521 tacttaccat gttgagttta atcctcctaa agtagaaggt gtatgtgatg tatgccaagg
    11581 agaattatat caaagagctg atgataatga agaaactgta tctaagagaa tacaagttta
    11641 tctagatgaa actaagcctt tagtagatta ttatagcaaa caaggtataa tagcagatat
    11701 aaaaggtgat caagcaatag ataaagtatt tgaagatatt gtcgcagcct taggaagtgg
    11761 aaaataaatg attatcttga aatcaaaaaa agaaatagaa cttttaagag aagcaggtaa
    11821 aatagttgct gacactcatg aagttttaag aaaagctatt tctccgggaa tatctacttt
    11881 agaattagat aaaatagctg aagagaatat aagaaaatac aatgcagaac cttcttttaa
    11941 aggatatgga ggttttccag gaagtatatg tgcttctatc aatagagagg tagtacatgg
    12001 tattccagga gaaactatat tacaagaagg tgacattgta agtttggata taggagccta
    12061 ttataaagga tatcatggtg attctgcgaa aactcacgga gtaggcatga tatctgaaga
    12121 agataggaaa ttgatagaag taactagaga aagcttctat gaaggaataa aatttgctaa
    12181 attaggatat agactttcag atatctcaca cgcagtacaa acacacgttg agaaacatgg
    12241 tttctcggta gttagagatt tagtgggtca tggagttgga gcaaatcttc atgaagaccc
    12301 acaagtacct aattatggac tcccaggaaa aggaccaaga cttagagaag gaatggttat
    12361 tgctatagag ccaatggtta atgcaggtcg ttatcatgta aagactctat cagatgggtg
    12421 gacgactgtt acaatagatg gaaaaaaatc agctcattat gagcatacga tagcaattac
    12481 tgaacatgaa cctttgatat tgacaaagtt gtaatttaaa aagtaggtga aaaaagtgct
    12541 atcagataat ttaagtatag gtcaagttgt taaagtttca ttaggacgag ataaaggaaa
    12601 tttattcttt gtagtcaaaa taattaataa tgaatatgta ttaatagctg atggaaaaaa
    12661 gagaaaactt gacaaaccta agttaaagaa agtgaagcac ttaaaaaaat ataattttat
    12721 taatgatgaa gttagaaaaa gagtagtatc tggacaagag attacagatt cttttttaag
    12781 agctgaactt actaagttaa attagataat ttaacttatt gaaatggagg gctggttagt
    12841 taatggccaa aaaagatgtt atagaattag aaggtacagt ttcagaagct ttacctaatg
    12901 ctatgttcaa agttaaacta gaaaatggac atgagatatt atgccacatt tctggaaagc
    12961 taagaatgaa cttcataaga attcttgaag gtgacaaggt gaatgttgaa ctttctccat
    13021 atgaccttac aagaggaaga attacttggc gtaagaagta gactatatta atatttagtc
    13081 taaggaggga ttaataatga aggttagacc atcagtaaaa ccaatatgtg aaaaatgtaa
    13141 agtaataaaa agaaaaggaa aagtaatggt tatctgtgaa aatccaaagc ataagcaaaa
    13201 gcaaggataa gaaattatta ctagcatttt gttaaatttt atagtataat actatattgt
    13261 gatgttaaca aaagaacgtt aaaatcgttg aacaatctgg cgtaggattg gcatatgatg
    13321 tgaagtttta aacactaaaa actatgtgta ggaggtgcga tcatggcaag aatagctggg
    13381 gtagatttac ctagagaaaa aagagcggag ataggcttaa cttatatata tggtataggt
    13441 aaagcaactg ctaacgaaat attagctaag gctgagataa atccagatac tagaataaaa
    13501 gatttatcag aagatcaagt taatgagtta agaaaagtta tagatgacga tttcttagtt
    13561 gaaggtgact taagaagaga aattgcttta aacataaaga gattaagaga tataaaatgt
    13621 tatagaggaa taagacacgc taaaggtctt ccattaagag gacaaagaac taagactaat
    13681 gctagaacta gaaaaggtcc tagaaaaaca gtatctcgta aaaagaaaaa ataaataaat
    13741 aataactaga tgaaggaggg aaaaggaaaa tggctaaacc aaaaaagaaa gttacacgta
    13801 ttagaagaag agaacgtaaa aatatagaac gtggtcatgc tcatatacaa tcaactttca
    13861 ataatacaat aataacttta actgacgttc acggaaatgc tatatcttgg gcaagttctg
    13921 gacaattagg attcaaagga tcaagaaaat caactccatt tgcatctcaa atggctgctg
    13981 aaacagctgc aaaagctgcg atggaacacg gactaaaaag tgttgaggta ttcgtaaagg
    14041 ggccaggttc aggaagagaa gctgcaataa gagctttaca agcaactgga ctagaagtaa
    14101 ctatgataaa agacgttact ccaatcccac ataacggatg cagaccacca aagagaagaa
    14161 gagtgtaatt aataagagta caataggagg tgtagataaa tggcaagata tacaggtgca
    14221 tcatgtagac aatgccgtag agagggaatg aaattattcc ttaaaggtga tagatgttat
    14281 acagacaaat gtgctatagt aaaaagaaac tatgctccag gtcaacatgg ccaaggaaga
    14341 aagaaagttt ctaactatgg attacaatta agagaaaaac aaaaagttaa aagaatatat
    14401 ggagttttag agactcaatt tagaaattta tatgaacgtg ctgaaaatat gcctggtaaa
    14461 gcaggtgaga acttattaag tttattagag agaagattag ataatgtagt ttacagaatg
    14521 ggactagcat cttctagaaa agaagctaga caattagtga ctcatggtca tttcacttta
    14581 aatggaaata aagtagacat accttcatta atagttaaag ttggagatgt tatagaagtt
    14641 aaagagaaat caagatcttc tgcaaaattc aaaaatttag tagaagttaa ctcaagaata
    14701 gctcctaagt ggttagaagc taatgtagaa ggaatgacag caaaagtagt tggtgttcca
    14761 acaagagaag atatagatct tgagatagca gagcacttaa tcatagaact ttactctaaa
    14821 taataagcac acttttaaaa attattttaa aattttgttt accctcagtg gattactgaa
    14881 ttttaaggag ggttttgtcc atgatagaaa tagaaaagcc aaaagtagat atagttgagc
    14941 ttagtgaaga ctatagatat ggtaagtttg ttatagaacc tctagaaaga ggatatggaa
    15001 taactatagg taacgcatta agaagaatat tattatcatc attaccaggt gtagcagtga
    15061 atgctataaa aatagatgga gttcttcatg aattttcaac aatacctggt gttaaagaag
    15121 atgttactga gataatatta actttaaaag agctttcagc aactatagat ggtgaaggaa
    15181 gtagaacact taaaatagaa gctcaaggac catgctctat cacaggtgca gatataatct
    15241 gtcctccaga tgttgaaata ttaagtaaag atttagcaat agctacatta gatgataatg
    15301 ctaaacttaa tatggagata tttgtagata aaggtagagg ttatgtttct gctgaagaaa
    15361 ataaaacaga gaatgttcca ataggtgttt tacctgtgga ttcaatatat actcctgttg
    15421 agaaggttag ttatcatgtg gaaaacacaa gagttggtca aaaaacagat tatgataaat
    15481 tagtactaga agtttggact aatggtagta taaatcctca agagggaata tcattagctg
    15541 caaaggtatt agttgagcat ttaaacttat ttatagactt aactgaacat gtaagtagtg
    15601 ttgaaatcat ggtagaaaaa gaagaagatc aaaaagaaaa agttcttgaa atgactatag
    15661 aagaattaga tttatcagtt agatcatata actgtttaaa gagagcggga attaatacag
    15721 ttgaagaatt agctaataaa tctgaggatg atatgatgaa ggttagaaac ctaggtaaga
    15781 agtcattaga agaggtaata caaaaactag aagaacttgg attgggatta aaaccaagtg
    15841 aagaatagcg agcaaaggag ggataagaat ggctaagtac cgtaaattag gacgtgaaac
    15901 agctcacaga aaccttatgt taagaaactt agtaacttgc ttactaagaa gtggaagaat
    15961 agaaactaca gtgactagag cgaaagaaac tcgtagaatg gctgaaaaga tgataactct
    16021 tgctaagaga ggagatcttc atgctagaag acaagtttta gcttatgtta tggatgaaac
    16081 agtagttaat aacttattca cagatttagc tccaaaatat gctgagagaa atggtggata
    16141 cactagaata ataaaaatag ggccaagaaa aggcgatgct gctgaaatgg cttttataga
    16201 attagtatag taaggaaagg attaggcttg atggcttaat ctttttttta tttttttgac
    16261 taaggtatga tataataatg tgtaaaatga tttttataag ttattgctta ctaaaaggag
    16321 tatgagttaa tggataatat agtaaaggta aataatattt cgtttgagta tattacagat
    16381 gaagcaaaac ttaaagcaat agataattta agcctagatg ttaaaaaagg agaatttgtt
    16441 gcgataatag gacataatgg ttctggtaag tctactttat ctaaaaactt aaatgctatt
    16501 cttatgccca ctgaaggaaa tattcttatt gatgatatgg atactaaaga agaagaaaga
    16561 ttatgggata taagacaaac tgcaggcatg gtatttcaaa atccagataa tcaaatagtt
    16621 gctactatag tagaagaaga cgttgcattt ggacctgaga acttaggtat agaaccaaaa
    16681 gaaataagac gaattgtaga ggagtcttta aaaagtgtag gtatgtatga tttaagagat
    16741 agacagcctc atttgttatc aggaggtcaa aagcaaagag ttgcaatagc aggaattatt
    16801 gctatgaggc ctaaatgtat aatctttgat gaagcaacag caatgttaga tccatctgga
    16861 agaaaagaag ttatgaaaac aataaaaaga cttaataagg aagaaaatat aactgttata
    16921 catataacgc actttatgga agaagcagta gaagctgata gagttgtagt tatggaaaaa
    16981 ggaaagaaaa tcttagaggg aactcctaga gaagtattca gtaaaataaa gatgttaaag
    17041 gaaatcggtt tagatgtacc ttgtatgaca gagttatcta gtttattaat agaagaaggt
    17101 ataaatatta gtagtgatat attaactgtg gatgagatgg tgatggaatt atgtcaatta
    17161 tagtaaagaa tttaacacat atatataatg aagggatgcc ttttgcaagt aaggcacttg
    17221 atgatgtttc ttttgagatt aaagatagag attttgttgg tcttataggt catacaggct
    17281 caggaaaatc tacattaata caacatttaa atggattact aaagccttcc tcaggagaaa
    17341 tatttataaa tgattttaat ataacagata aaaacttgaa tttaactgag attagaaaaa
    17401 gagtaggggt tgtatttcaa tatccagaat accaattgtt tgaagaaacg attgataaag
    17461 acatagcttt tggaccctct aatttaggct tagaggagtc tgagatacat aacagggtaa
    17521 aagcatcaat ggaagctgta ggacttgatt atgagggatt taaagacaaa tctccttttg
    17581 aattatctgg gggacaaaag cgtagagtag caattgctgg agttatagcc atgaacccag
    17641 aagttctcat attggatgaa ccaactgctg gtcttgaccc tggtggaaga gatgaaattt
    17701 ttaacttaat aaaagactta catgaaaaaa agaatatgac tataatacta tcatcacata
    17761 gtatggatga tatggcaaag ttagcaaaaa ctttaattgt tatgaatcat ggtagcgtag
    17821 aatttatggg aactcctaga gaagtattta aatcaaatgc tagtaaatta aaagatatag
    17881 gattagatat tccacaagtt ttagagcttg cattgaaatt gagagaaaaa ggctttgaca
    17941 tcagtgagga tatattgact ttagaagaag caaaacagga aatattaaaa gttgtgagag
    18001 gacgaggatt atgttaaaag atataactat agggcaatac tacccaacaa gttctgctat
    18061 tcataaatta gatccaagga taaagcttgt agcgacaatt gtatttatgg tatctatatt
    18121 tgtagtaaat aaattttggc cttatatagt agttttatta tgtttattag ctatgataaa
    18181 attggctaat ataccagtca aatatattgt taagggtgtt aaacctctta aatggattat
    18241 tttgtttaca tttcttataa atattttttt cttaccaggt gatgaaatat ggtcatttgg
    18301 atttttagct attacaaaac agggtctaag acaagcaatt tttatggcaa taagattaat
    18361 atttttagta gttggtacat cattgcttac tttaacaact tcaccaatag aattaactga
    18421 tggaatagag agattgttga atccatttaa gaaaattggt ttgccagttc atgaacttgc
    18481 aatgatgatg acaattgctt tacgttttat tcctacattg ttagatgaga cagacaaaat
    18541 aatgaaggct cagatgtcta gaggtgcaga ttttgaaagt aagaatctta taaatagagc
    18601 aaagaattta gtaccacttt tggtaccttt atttgtaagt gcatttagaa gagctgatga
    18661 attggctatg gccatggagg ccagatgtta cagaggtgga cataatagga ctaagatgag
    18721 agagtctgtt atatcaagaa gggattatat ggcatgtgta tttcaggttg tttatctggg
    18781 agcaattata gctactagat ttatagcaat ttaattaata agttatagat ataatgaggt
    18841 aaagtatgag aaatataaaa attaaaatcc aatacaatgg taagaattat tgtggatggc
    18901 aaaagcaacc tgattcttta gggatacaag gtacaattga aagggctata tatgatataa
    18961 caaaagaaga aaccagtctt atagggtctg gtagaactga ttcaggagtt catgctattg
    19021 gtcagattgc aaattttaaa attaattcag gtataagtat agaaagtata ccaatggcac
    19081 taaatgccaa gttgccaaaa gacatatcag ttatagaagc atgtgaagta aatgatgatt
    19141 ttcactccag atacagtgca aaaggtaaaa catataaata tttagtatat aatagcaagt
    19201 ttagaaatcc aatacttagt gagatatcat atcaagtaaa atatgaactt gattttgata
    19261 aaatgtgttc agaagcaaaa agtttattag gaacacatga ttttaaaggt tttatgagtt
    19321 ctggttcatc tgtaaaagat acagttagaa cgatatatga tattgatata agtaaaaaag
    19381 atgacttgat tacttttgag attagtggta atggtttttt atataacatg gtgaggatta
    19441 ttgttggaac acttgttgat atgggtcgtg gcagaattaa cgaaccattt ttagatataa
    19501 tacaatcaaa aactaggtca agatgtggac atacagctcc agcacaagga ttatttctca
    19561 aaaaagttca ttattaactt gacacaccag gctacatgta ttaaaatata tgagaatgtg
    19621 ttaataagtc cacttgcccc ggacttgaca taaacggtaa aaaaatttta tgaaaagtag
    19681 ttaaggaggg acagttatga aaagttatat agctaagcca gctgatgtac aaagaaaatg
    19741 gtacttagtt gatgctgaag gaaaaacatt aggtcgttta gcaacagaaa ttgcgacagt
    19801 attgagaggt aaacataaac caacatttac tcctcacgtt gatggtggag attttgttat
    19861 tgtagtaaat gcagaaaaaa tagttttaag tggtaagaaa ttagatcaaa aatattacag
    19921 ataccatact ggttatgtag gtggattaaa agaaatatct tacagagata tgatggataa
    19981 gaaaccagaa gaagttatat cacatgctgt aagcggtatg ttacctaaaa ataaattaag
    20041 aagcagaatg atgactagat taagagtatt tgcaggtgct gagcacactc atgcagctca
    20101 aaatccagaa gttttaaact ttaaataaga ttaataggtg aaaggaggag tttataatgg
    20161 ctaacgttca atattatgga actggaagaa gaaaaagttc tgttgctaga gtaagactag
    20221 ttgcaggtga aggaaatata ttagtaaatg gaagagcatt agaaaattat tttaattatg
    20281 agacattaat aagagatgtt aaacaaccat tagttttaac tggtaatgaa aataaatatg
    20341 atgttatagt aaaagttgaa ggtggaggat tcactggaca agctggagct ataagacatg
    20401 gaatatctag agctttatta aaagctgatt tagatttaag acctgcttta aagaaagaag
    20461 gtttcttaac tagagatgca agaatgaagg aaagaaagaa atacggatta aaagcagcaa
    20521 gaagagctcc acaattctca aaaagataat tacttatttt tttggaaaag caaaatcctt
    20581 tatttactgt tattcagaaa gtattggtat tttaaaaatg ttgggttaag ttaattattg
    20641 atatataata aaataataag atatcttatt aggatgtttt tcattaagaa gaacatcctt
    20701 tttattttga ctatttaata aaaaacatat gctaataaca tttaaatttt attttta
//
LOCUS       DAELLV010000044        20351 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.044, whole genome shotgun sequence.
ACCESSION   DAELLV010000044 DAELLV010000000
VERSION     DAELLV010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20351
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.044"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            366..1559
                     /gene="nifV"
                     /locus_tag="KPD58_001368"
     CDS             366..1559
                     /gene="nifV"
                     /locus_tag="KPD58_001368"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HBG0265147.1"
                     /translation="MCVISKDRAKEIKIVDTTLRDGEQTAGVVFANREKIMIAEMLSD
                     LGVDQIEVGIPTMGGDEKNVIKHICSRNLKSDIMAWNRAVIKDVEESISCGVDAVAIS
                     ISVSDIHIENKLRTSRGWVLENMAKTVEFAKKNGLYVSVNGEDASRADIDFLTEFINV
                     GKQAGADRFRYCDTVGVMNPFSIKNAIETLYERTNFDIEMHTHNDFGMATANALAGIA
                     AGANYVGVTVNGLGERAGNAALEEVLMALKCVYKCDLNNIDTRKFRGICEYVAQASGR
                     ILPTWKPVVGDNMFIHESGIHADGALKDPHNYEPFDPSEVNLERKIVIGKHSGRAAVV
                     NKLSEYEMYISPENATKLLNAIRATSIRLKRSLMDKEILQLYCDILAHEKGTTEEEAV
                     RGSYI"
     gene            1584..3509
                     /locus_tag="KPD58_001369"
     CDS             1584..3509
                     /locus_tag="KPD58_001369"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HBG0265148.1"
                     /translation="MGDNIVYKIIKKHIVDGEAVAGSSIGIKIDQTLTQDSTGTMTYL
                     QLEAMGIDKVKTKRSVAFVDHNMLQQGFENADDHKYIQTVADKYGVYFSKPGNGICHQ
                     VFLERFSTPGDTLLGSDSHTPTAGGVGMMAIGAGGLDVALAMAGGAYYIKAPKVCKVN
                     LVGKLNNMVSSKDIILEVLRKQTVKGGVGKVYEYGGEGVKSLSVPQRATITNMGAELG
                     ATTSIFPSDEKTLEFFKSQGREDAWVELKPDADAVYDEEITINLDELKPLAAKPHSPD
                     NVDEVENIGKIKIDQVAIGSCTNSSYEDLMKVAQILKGNKVHKDVSLVIAPGSRQVME
                     MIARNGALADIISAGARILENSCGPCIGMGQSPGTDSVSLRTFNRNFYGRSGTLSAQV
                     YLVSPEVAAVSAIKGVLTDPREFDIKFTNLDVNEFLIDDSMIIKPADVGSDVEVVRGP
                     NIKPFPLNTELSQSIGGKVILKTEDNITTDHIMPSNAKLLPFRSNIPYLANYCFNTVD
                     TEFPQRAKDNNGGFIVGGDNYGQGSSREHAALAPLYLGVKGVIVKSFARIHKANLINS
                     GIIPMEFCDEKDYENISLLDNLEIPNILDNLGSGILEVKNTTKGTSFKVKVELSAKEV
                     DVLKAGGKLNYTKNQAN"
     gene            3531..4526
                     /locus_tag="KPD58_001370"
     CDS             3531..4526
                     /locus_tag="KPD58_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HBG0265149.1"
                     /translation="MYKVTLIPGDGIGPEVAKAMKKVVEATGVEIEWEEVNAGEAVIE
                     EYGTPLPEYIIDSIKKNKIAIKGPITTPVGKGFRSVNVTLRQALDLYVNLRPIKSFKG
                     IKSRYEDVDLVVVRENTEDLYAGIEHKIGDYAAESIKIITRSASERIVDFACNYVKDN
                     KRKKVTAIHKANIMKMSDGLFLDVFREVASKHGVEYDDLIVDAAAMNLVLNPENYDVM
                     VMPNLYGDILSDLGAGLVGGLGIIPSANIGKDCAIFEAVHGSAPQIAGQNKANPTALI
                     QSSVMMLRYLGEYENAQKIETALEKVFLEGSKLTVDLGGSASTTEFADEVCKYIV"
     gene            4852..5325
                     /locus_tag="KPD58_001371"
     CDS             4852..5325
                     /locus_tag="KPD58_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0265150.1"
                     /translation="MVVSVEVCLIKTLDSNILREVGTLSRAVNSINDIKYKELKLQKG
                     QFTFLTRICENPGINLVELSNMLKVDKATTTKAIQKLIKAGYVDKKQDEFDKRGYNLT
                     PTDKSLEVYELIIEEENRSIEICFDNFTDEEKQVVTKLLEKMSKNVENEWFKVKR"
     gene            5359..5853
                     /locus_tag="KPD58_001372"
     CDS             5359..5853
                     /locus_tag="KPD58_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0265151.1"
                     /translation="MIRKANMNDLESIMKIIKSTVEEMKTYNNTQWDENYPLEKDFVS
                     DIKKQDLYIYEVDGEVAGFICLNYEEPEEYLGLNWSSNKKAMVIHRMAVNPNFRKTGI
                     ASKLVDFAEKLAVENNVSYLKSDTYSINSKMNLLLTKCGFIKIGEMSFLGKEKSFYCY
                     DKIL"
     gene            5950..6471
                     /locus_tag="KPD58_001373"
     CDS             5950..6471
                     /locus_tag="KPD58_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HBG0265152.1"
                     /translation="MIGFLKKRRSIRKYKDVEVEKEKLDKILKAALLAPSSKGLRTWE
                     FIVVDDKEKLINLSQCRTKGGGFFLKNAPLAIVIIADKEKNDVWIEDASIAASYIQLQ
                     AHELGLGSCWIQVRNRMHDDNIEADKYIREELKVPSKYSVECIISIGYPDEEKKAYSD
                     SDLDYKKVHFNNF"
     gene            6660..6971
                     /locus_tag="KPD58_001374"
     CDS             6660..6971
                     /locus_tag="KPD58_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG0265153.1"
                     /translation="MGIFKTKMDEDWKVNYIKEFNEMRDSYESKLQKKQFEVDSLKSE
                     LDRLRSYKNSLKPKEKQITDDDINNIKSLRRDGLSYKEISNQTSWSKATVSRVLNGLY
                     D"
     gene            6999..7685
                     /locus_tag="KPD58_001375"
     CDS             6999..7685
                     /locus_tag="KPD58_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG0265154.1"
                     /translation="MKYSYKKCIIDSILLMFIVQILRMILNYVLLSQFEFTLENFNII
                     NLISFTLVGLSLILFLKDNSLYNKVRNRKITEAFEENKNNILIEKCKLILFVVVLSLA
                     IIVTYCTKGYVLFNVTMMTLSVLIVPIFEELFFREYIWNYLSNFIKSKSKIICITSIL
                     SGIYNIGYIDVIRNYVILYNNSSYTFEVIISKIMIGTVFGIVLGLVKYRFRDVGFCIL
                     LRSLFAIFIR"
     gene            7917..8801
                     /locus_tag="KPD58_001376"
     CDS             7917..8801
                     /locus_tag="KPD58_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBG0265155.1"
                     /translation="MKFVTFCSSNEEKIGVFNSETNSIYEINSLGLSKLYTDMNNFIE
                     NVSTGDLEKIKNNSFENAKCYKLEEVKLCSPIVRPKKDIICLGLNYKDHVNEIPDGVI
                     KNVVMPDYPIYFSKRADKIIGVDDKISLHGDLVEKLDYESELAVIIGKEGINISKEDA
                     YEYIFGYTIVNDISERALQDKHVQWFRGKSLDTHTSMGPYIVHKEEFEHPLKLDISSV
                     VNGEVRQNSNTEYFIFDIPTIINDLSRGMTLKPGDIISTGTPAGVAMGMNPQVYLKHG
                     DIVECKVEGIGVLKNIVD"
     gene            8900..10075
                     /locus_tag="KPD58_001377"
     CDS             8900..10075
                     /locus_tag="KPD58_001377"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HBG0265156.1"
                     /translation="MARELELKYGCNPNQKPSKIYMKNGELPIEVLNGKPGYINFLDA
                     FNSWQLVKELKEATGLPAATSFKHVSPAGAAVGVPLSDTLKQIYFVDDLELSPLACAY
                     AMARGADRMSSYGDFIALSDVCDKETATIIAREVSDGIIAPGYTDEALEILKGKRKGN
                     YNIVKIDENYTPEPIETKDVYGITFEQGRNEILINEDLLKDIPTDNKIFTDSAKRDLI
                     IALITLKYTQSNSVCYAKDGQVIGVGAGQQSRIHCTRLAGNKADTWYLRQHPKVLNLK
                     FKKDIGRPDRDNTIDVYLSDDYMDVLADGIWQNFFEEKPEPLTGEGKRAWLKTLTGVA
                     LGSDAFFPFGDNIERAKRSGVSFIAQPGGSIRDDNVILTCNKYNIVMAFTKNRLFHH"
     gene            10203..11057
                     /locus_tag="KPD58_001378"
     CDS             10203..11057
                     /locus_tag="KPD58_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265157.1"
                     /translation="MALNIRNYSLLCNQGTAKFNEDVVGINPFGAWVLDGATGLNGKN
                     LVSTESDARWYVQWWNRYLYKNINKEESLKSIINTGIIEVKKEYELRLNGFKVEKLDL
                     PSSSIVVVKFHENKVEYFLLGDCALFIKNGDSKVIKDRSICKFDNTVFDEMSKLSNLE
                     EMAFDEIRTSVMDTIVSNRLKKNTKEGYWILDFDEEAVENSIYGYIEIKKDFQLMLTS
                     DGFTSACDRYNLIKEEELIRIAEKFGIGYIHNKVRDFEDNDYKAVKVPRFKVKDDSSC
                     LYLDIYND"
     gene            11101..12336
                     /locus_tag="KPD58_001379"
     CDS             11101..12336
                     /locus_tag="KPD58_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBG0265158.1"
                     /translation="MKILHIITQKPNSTGSGIYLSGMIKGFEKIGHKQAVIAGIDVND
                     DVNCFPSEVSFYPVKYNCGELNFPVVGMSDSMPYESTRYKDLNIDMINRLKYQFKVNI
                     DKAMNDFKPELIICHHLYLLTAFVREMVKDIKVMSICHGTCLRQLNTIDLEKEYIIAN
                     IRKLDLIFALHENQKYDIIKTFGVSESKVVVIGSGYNDDIFYNKNYKIKDDKIKIVFA
                     GKICKSKGLIPFIKAISKLKYSKDLIEVNFAGTGSDIESYNEIVKLASKSPFKMNFLG
                     KLEQRDLAELFNRSQIFVLPSFYEGLPVVVLEALSCGTDVITTDILGVKEWIGSEINN
                     SGKIEYISLPFMEKEGIPKDEELYDFENNLYNAIDSKIQSLLNNSNKKTSVDMSKKTW
                     DGLAYRINEVILMDELCLV"
     gene            12437..13378
                     /locus_tag="KPD58_001380"
     CDS             12437..13378
                     /locus_tag="KPD58_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp25"
                     /protein_id="HBG0265159.1"
                     /translation="MRISKKVLALGISALLLSVSFPTSINALDKIENIQGVDKYETAG
                     LISDKQDFTTAVLINADSTMVDGIAASGLAGVNNAAILLTNKDDIPEATLQRLNRVTK
                     IYVIGGENSISKDVEKMLLMRRMQVIRIDGVDRVDTSYKIAGEIEKIKNSDKMFLVNG
                     FKDEADAVSVASVAYRDGAPIILTKDIPSAEEDTDLDPWFGVSPVPVYAIGGESTLSD
                     YIVSRYRATRIGGVDRYQTNKNVIEKFYNGAKEFYITSGDDLVYALVASPLAKNAPVV
                     LVSNKSDKSILSGASKVTAIGISDKSIIEQCLDAVKK"
     gene            complement(13429..13788)
                     /locus_tag="KPD58_001381"
     CDS             complement(13429..13788)
                     /locus_tag="KPD58_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265160.1"
                     /translation="MDLFEVNIVLNSGTAGGMNPKLKIFNMVISTEVCHHDVTQDTLT
                     EFHSWLKTVFFEANCELIKISEMAVKKTNQEETVFWGRILAGEAFITNKGRKKINEKE
                     AYLHLIASSHIFYTIIK"
     gene            complement(13814..14278)
                     /locus_tag="KPD58_001382"
     CDS             complement(13814..14278)
                     /locus_tag="KPD58_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0265161.1"
                     /translation="MNLGNSLFQARKKHGLSQEDVAEKLGVSRQTVSKWETDETVPDI
                     RQSKKLAILYNVSLDELIDFDIDMEEIQEAINKTSEETEEKINWTNAWGKKYPILLKY
                     QTDVNISNYAYKVNMMLDELKQEYQYNELEAMLILKDILYNVWKNRKSKRKQ"
     gene            complement(14446..14654)
                     /locus_tag="KPD58_001383"
                     /pseudo
     CDS             complement(14446..14654)
                     /locus_tag="KPD58_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004071281.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
     gene            complement(14698..15051)
                     /locus_tag="KPD58_001384"
     CDS             complement(14698..15051)
                     /locus_tag="KPD58_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBG0265162.1"
                     /translation="MDRKRELKQLYKEMKFDTGVFIIKNDITKKIYLGKSNDIKSKFN
                     SLKFQLGAGSCMIKELNNEWKKYGEKAFTFEVLELIKHDDNKTEKDYLEELDILEMVW
                     LEKLKEDNTYEIYSI"
     gene            15347..16258
                     /locus_tag="KPD58_001385"
     CDS             15347..16258
                     /locus_tag="KPD58_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HBG0265163.1"
                     /translation="MNKKIELIGNFASDWIRYSDYEYKITEEDELYIVPTEDAVFTMY
                     NPFDVADDIIVDIIRIGQEALQDISKETKEMKLKKEILEFVKKYGLLGLIYSSVYNRD
                     IVGEERVLMIEKNYITKENILSTDEYVNMFIPFASDEDVYFKKYRRGIDVSKREESPK
                     FFGKRPLILDLVFSKFYTEQLDWIVSFAKDMCKHFNQICVYKNTSNLLTESVTIMSGG
                     FNPQKIGFSISQLDKPIIAWEFDSLKTAIETIYAFAITSEPSSLNKCKYCGKIYIPTN
                     SKSQYCSPSCRNCSNVKKSRSRAKKDK"
     gene            16519..16953
                     /locus_tag="KPD58_001386"
     CDS             16519..16953
                     /locus_tag="KPD58_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265164.1"
                     /translation="MISPIILSSINQNLKEIERNELLETNIESGDYGLALSESDVKDI
                     INSRDNTLKGYGRIELDIKVTKQLIENIYTSQYTNVDNYLEAINDMQEIFYYLKNETD
                     DKICDDEIIEILGDFYEKFSGNMDNVRGEADEFAKKFKFGEV"
     gene            16976..18511
                     /locus_tag="KPD58_001387"
     CDS             16976..18511
                     /locus_tag="KPD58_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265165.1"
                     /translation="MLNNLSNININENNLIKNQYSISLLKECLNCKVIDEREVYNIQQ
                     EISLILMDLIKKYTNGQSTSVKTEVAEKLLISIWYAIDAYINKLGDIEKRVVELTAKN
                     VKKVYLGGIEILEEELVRLKDFYKLMMSRKLDTNSIAYNDTLMELSSFFKSYIVKFEA
                     QDVPVSIDYPLALDDMDVKGIYYVKNYIESIDIENRFCNLFKKKDREKLFYDYGVTYK
                     IDCDVILVNLFELIMNNCIFSTILGNEVTNLEISKYEYEFLEKQFKNMYNNITEDKVI
                     DSEESNIIELIEVDDSFNTEVDSNLDYNDFEEINREIRINKKITLLLMEAIEVLIKRL
                     SIEDEDVIRYIKKYEKIFIESVLSSIKSNTFKSMVTVSKNKNEHLEDYIVDDESKLDD
                     ESFREIFNEILDSTSIIEKIRIIKENINAKKDFIDILESECLFGEEYLMLFASLSELE
                     LAILGSVVFYEDIRMREINILEFMLNEKTETTLWKIEYIKFMKRQSEDKIYSIEEHMN
                     KIN"
     gene            18595..19962
                     /locus_tag="KPD58_001388"
     CDS             18595..19962
                     /locus_tag="KPD58_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0265166.1"
                     /translation="MKEYIINNVEEIREELIDLSKKIWENPELAFEEKYASSIQKEYL
                     KSKGFKIEEVENLPTGFIASFGEGKPVIGILGEYDALPELSQCVSAERKPLVEGKAGH
                     GCGHNLLGVAGVGAVVSIKKLIEENKFNGTIKYFGCPAEEEGGGKTVMCINGCFDDVD
                     CAFTWHPFDINAPWRGGSLANLSVKFKFKGITAHAAQAPHNGRSALDAVEIMNVGANY
                     LREHVIDSIRMHYVITNGGGRPNVVPGFAESWYFIRGKKAKDAEHVLDRLIKVAQGAA
                     MMTETEMEYKVTDGIYDYIPNQCLTDLVYDNMVFVGCPKNTPEEEEFAKKLCDTLTRE
                     ERLGVATSLQNDKSIVESYIHKEIVDGDKDKGLAGSTDVGDVSYVIPVAQFAMAAWPV
                     GIASHTWQSCSSAGSNIGFSAMINSAKVLACSAYDVFMDTKIIDEAKIEFDKSLDGQK
                     FKPLV"
     gene            19997..20221
                     /locus_tag="KPD58_001389"
     CDS             19997..20221
                     /locus_tag="KPD58_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HBG0265167.1"
                     /translation="MREKVEKVLEEKIKPVLQRDGGDVELIDVNENGVVLVRLQGACS
                     GCPGATMTIKAIIENVLVSEVPGVTQVLGV"
BASE COUNT         8010 a         2045 c         3737 g         6559 t
ORIGIN      
        1 tatagcttaa aaatatatta agatacttta aaatggaatg taacttaaat taaattttag
       61 taggaatttt ataggtttat ttttaaaggt tttttgaaaa taaatctatt taaaaattaa
      121 attaaaatgc aagaaaaaca taataacttt catttttaag aagttttcac aaaaaagaaa
      181 aaatatagtg caataaatga tacaaagtaa tataatagat tacaagatat gaaaagctaa
      241 aaaaataaat tgcaaatgaa attaaaatga ataaaaaata aaaaaataat cataagttag
      301 aggtatcaat gaaaaataag acttaatgtt tttaaaatta aaagtatact aaggggggca
      361 ttaatatgtg cgttatatca aaagatagag caaaggaaat aaaaatagta gatactactt
      421 taagagatgg agagcaaaca gctggggttg tatttgcaaa tagagaaaaa ataatgattg
      481 ctgaaatgtt aagtgaccta ggtgttgacc aaattgaagt tggtatacct actatgggtg
      541 gagatgaaaa aaatgtaata aagcacatat gtagtagaaa tttaaaatca gatataatgg
      601 cttggaatag agcagtaata aaagatgttg aagaatctat ttcttgtggt gttgatgcag
      661 tagctatatc aatatcagta tctgacattc atatagaaaa taaattgaga acttctagag
      721 gctgggtttt agaaaatatg gctaaaacag ttgaatttgc aaagaaaaat ggattatatg
      781 tttcagtaaa tggagaagat gcttcaagag cagatataga cttcttaact gaatttataa
      841 atgttggaaa acaagcaggg gcagatagat ttagatactg tgatacagta ggagttatga
      901 atcctttttc tataaagaat gcaatagaaa ctctttatga aagaactaat tttgatatag
      961 aaatgcatac acataatgac tttgggatgg caactgcaaa tgcacttgca ggaatagcag
     1021 caggagcaaa ttatgttggt gttacagtaa atggattagg tgaaagagca ggaaatgcag
     1081 cattagaaga agttttaatg gcacttaagt gtgtttataa atgtgattta aataatatag
     1141 atactagaaa atttagagga atatgtgagt atgtagcaca ggcatcagga agaatacttc
     1201 ctacatggaa accagtagtt ggggataata tgtttataca tgaatctggt atacatgcag
     1261 atggagcttt aaaagaccca cataactatg aaccatttga cccaagtgaa gtaaatcttg
     1321 aaagaaaaat agtaataggt aaacattcgg gaagagctgc agttgtaaat aagttgagtg
     1381 aatatgaaat gtatatttct ccagaaaacg ctaccaagct attaaatgct ataagagcta
     1441 catctataag attaaaaaga agtttaatgg ataaagaaat tcttcaatta tattgtgata
     1501 tactagcaca tgagaaaggc acaacagaag aagaagcagt tagaggttca tatatttaag
     1561 atataattgt ttaggaggaa aatatgggag ataatatagt ttataaaata attaaaaaac
     1621 atatagtaga tggagaagct gttgctggaa gttcaattgg tataaaaata gaccaaactt
     1681 taacacaaga ttctactgga acaatgacat atcttcaact agaagctatg ggaatagata
     1741 aagtaaagac taaaagaagt gtggcatttg tagaccataa tatgttacaa caaggatttg
     1801 aaaatgcaga cgaccataag tatatacaaa cagtggcaga taaatatgga gtttatttct
     1861 ctaagccagg aaatggtatt tgtcatcaag tatttttaga aagattttca acaccaggag
     1921 atacactttt aggttctgat agccacacac ctactgctgg aggtgtgggc atgatggcta
     1981 ttggagctgg tggattagat gttgctttag ccatggcagg aggagcatac tatataaaag
     2041 caccaaaagt ctgtaaggtg aatctagttg gaaaattaaa taatatggta tcatcaaaag
     2101 atataatatt agaagtgtta agaaaacaaa ctgtaaaagg tggagtaggt aaagtttatg
     2161 aatatggtgg agaaggtgtc aaaagcctat ctgttccaca aagagctaca ataacaaata
     2221 tgggggctga acttggagca actacttcaa tcttcccaag tgatgaaaaa acactagaat
     2281 tttttaagag tcaaggaaga gaagacgcat gggtagagct aaaaccagat gcagatgcag
     2341 tttatgatga agagattact ataaacttgg atgaattaaa accattagct gcaaaacctc
     2401 atagcccaga caatgtagat gaagttgaaa atataggcaa gattaaaata gaccaagtgg
     2461 caataggaag ttgtacaaat tcttcttatg aagacttaat gaaagtagca caaatcctga
     2521 aaggaaataa agtacataag gatgtgagtt tggtaatagc accaggttca agacaagtta
     2581 tggagatgat tgctagaaat ggggctttgg cagatataat aagtgctgga gctagaatac
     2641 tagaaaattc ttgtggtcca tgtattggta tgggtcaatc tccaggtact gactctgtgt
     2701 ctttaagaac tttcaataga aatttctatg gtagaagtgg gactttgtca gcacaagtat
     2761 atttagtaag tccagaagtg gcagcagtaa gtgcaatcaa gggagtgctt acagacccaa
     2821 gagaatttga tattaaattt acaaatttag atgtaaatga atttttgata gatgatagca
     2881 tgataattaa acctgctgat gtaggaagtg atgtagaagt agttagagga cctaatataa
     2941 aacctttccc tcttaataca gagctaagtc agtctatagg tggaaaagtt atattaaaga
     3001 cagaagataa tataactact gaccatataa tgccatcaaa tgctaagtta cttcctttta
     3061 gaagtaatat accgtatctt gcaaactatt gtttcaatac tgtagataca gaatttcctc
     3121 agagagcaaa agacaataat ggtggattta tagttggtgg agataattat ggtcaaggtt
     3181 caagtagaga acatgcagct ttagcaccat tatatctagg tgttaaaggg gtaatagtta
     3241 agagctttgc aagaatccac aaggctaact tgataaatag tggtataatt ccaatggagt
     3301 tttgtgatga gaaagattat gaaaatatat ctcttttaga taatctggag ataccaaata
     3361 ttttagacaa tctaggtagt ggaattttag aagttaagaa tacaactaaa ggaacttctt
     3421 ttaaggtcaa agtagaattg tcagcaaaag aagttgatgt attaaaagct ggtggaaaat
     3481 taaactacac taaaaatcaa gctaattaat atgaaaagag ggaatattga atgtataaag
     3541 tgacacttat accaggagat ggaataggac cagaagttgc gaaagctatg aaaaaggtag
     3601 tagaagctac tggagtagaa atagaatggg aagaagttaa tgcaggagag gcagttattg
     3661 aagagtatgg tactccactt cctgaatata ttatagatag tataaaaaag aacaaaatag
     3721 ctataaaagg cccaattact actcctgtag gtaaaggatt taggagtgta aatgtaactt
     3781 taagacaagc attagatttg tatgtcaatt taagacctat taaaagtttt aaggggataa
     3841 aatcaagata tgaagatgtt gatttagtgg ttgtaagaga aaatacagaa gatttatatg
     3901 ctggaataga acataaaata ggtgattatg cagctgaaag tataaagata ataacaagaa
     3961 gtgcaagtga aagaatcgtt gattttgcat gtaactatgt taaagataat aagagaaaaa
     4021 aagttacagc tatacataag gcaaatataa tgaaaatgtc tgatggattg tttttagatg
     4081 tgtttagaga agttgcatca aaacatggtg tagaatatga tgatttgatt gtagatgcag
     4141 cagctatgaa cttagtttta aatccagaaa attatgatgt aatggtaatg ccaaatcttt
     4201 atggagatat actatcagat ttaggtgctg ggcttgttgg aggtttaggt ataattccaa
     4261 gtgcaaatat tggtaaagat tgtgcgatat ttgaagctgt tcatggaagt gctccacaaa
     4321 tagcaggtca aaataaggct aatccaacag cacttattca atcatcagta atgatgctta
     4381 gatacttagg tgaatatgaa aatgcacaaa aaatagaaac agctttagaa aaagtatttc
     4441 ttgaaggaag taaattgact gtagatttag gtggctcagc ttctactaca gagtttgcgg
     4501 atgaggtgtg taagtatatt gtatagattt tatttaggga aaaagtaaaa tatagattga
     4561 agaaaagagg ctttctcaaa atagagataa gcctttttta ttggaatgat aaaatatatt
     4621 tgattttata caaaagagag ttcctcataa acgaaaaagt taattttggg atatccattt
     4681 taattttaaa attaattgat taatatgcaa agaaagatta aataaattta ttatttttaa
     4741 ttgaactttc aaccaaaata tggtaaaatt taaaaaagtt gaactttcaa ctaaaaatga
     4801 atatattgat tatgtgaaat tctataaaac agaaactatt tgactttagc catagtagtt
     4861 tcagtggagg tgtgtttgat taagacttta gacagtaata tattaagaga agtaggtact
     4921 ttatctagag cagtaaattc aataaatgat attaagtaca aggaattaaa gttacaaaaa
     4981 ggacaattta catttttgac aagaatctgt gaaaatcctg gtataaatct tgtggaatta
     5041 tcaaatatgt taaaggtaga caaggctaca acaaccaagg ctatacaaaa attaataaag
     5101 gcaggatatg tagataaaaa gcaggatgag tttgataaaa gaggatataa tttaactcca
     5161 actgataaat ctttggaagt ttatgagttg ataattgaag aagagaatag aagtatagag
     5221 atatgttttg ataattttac agatgaagaa aaacaagtgg taactaagtt attagaaaaa
     5281 atgagcaaaa acgtagaaaa tgaatggttt aaagtaaaga gatagtaaat aaatacatta
     5341 acttaaggag atatttttat gattagaaaa gcgaatatga atgatttaga aagcattatg
     5401 aaaattataa aatctacagt ggaagaaatg aagacttata ataatacaca atgggatgaa
     5461 aactatccat tagaaaaaga ctttgtttca gatattaaga aacaagattt atatatttat
     5521 gaagtggatg gagaagtagc agggtttata tgtcttaatt atgaagagcc agaagaatat
     5581 ttaggcttaa attggtcatc taataaaaaa gcaatggtaa tacatagaat ggctgtaaat
     5641 ccaaacttta gaaagacagg gatagcatct aagttagttg attttgctga aaaattagct
     5701 gtagaaaaca atgtatcata cttaaagagc gatacatatt caataaactc aaaaatgaat
     5761 ttattactta caaagtgtgg ttttataaaa atcggagaaa tgagtttttt aggaaaggag
     5821 aaatctttct actgttatga taaaatatta taaagagcat ttacaataca aggggtaaat
     5881 aatagatttt cagcattagt aagggcataa tataacttag tatatgctat tatgaaaggg
     5941 atgatttata tgattggatt tctaaagaaa agaagaagta ttagaaaata caaggatgtt
     6001 gaagtagaaa aagaaaaatt agataaaata ttaaaagctg ctcttttagc tccatcatca
     6061 aaggggctta gaacatggga atttatagtg gtagatgata aagaaaagct aattaattta
     6121 tctcaatgta gaactaaagg tggtggattt ttcttaaaaa atgcaccttt agctattgta
     6181 attattgcag ataaagaaaa aaatgatgtt tggatagagg atgcaagtat agctgcttca
     6241 tacatacaac ttcaagctca tgaactaggt ttaggttctt gttggataca agtaagaaat
     6301 agaatgcatg atgataatat agaagctgat aagtacatta gagaagaatt aaaagtacct
     6361 agcaagtaca gtgtagaatg tataatttct ataggatatc ctgatgaaga aaagaaagca
     6421 tacagtgact ctgatttaga ctataaaaag gttcatttta acaactttta gtatataata
     6481 tgttgtatta agacattata tttttttcta ttgttaatat atacttaaaa ttgtaaaaat
     6541 atatctgaat tttactaact tatctagggg ttatgtacaa ggattttaaa gataaatata
     6601 gaaattaatg tttataatcg atacataact tgagtaacaa aatttaagga ggttaaagta
     6661 tgggtatatt taaaacaaaa atggatgagg actggaaagt aaactacatt aaagagttta
     6721 atgaaatgag agattcttat gagagtaaac ttcaaaaaaa acagtttgag gtagatagtt
     6781 tgaaatcaga attggataga ctaagaagct ataaaaatag ccttaagcca aaagaaaaac
     6841 aaattactga tgatgatata aataatataa agagtttaag aagagatggc ttaagctata
     6901 aagaaatttc aaatcaaaca agttggagca aagcaactgt aagtagggta ttaaatggac
     6961 tttacgatta gcatatacat ggggtgattt aaattgggat gaagtatagt tacaaaaaat
     7021 gcataataga ttctatttta ttgatgttta tagttcaaat attaaggatg attttgaatt
     7081 atgtacttct aagtcaattt gaatttactc ttgaaaattt taatattata aacttaattt
     7141 cttttacatt agttggttta tcgttaattt tatttttaaa agataattct ttgtataaca
     7201 aggttagaaa tagaaaaatc acagaagctt ttgaagaaaa taaaaacaat atacttattg
     7261 aaaagtgtaa gctgatactt tttgttgtgg ttttatcttt agcaataatt gtaacttatt
     7321 gtactaaggg atatgtactt tttaatgtaa ctatgatgac tttgtctgta ttgatagttc
     7381 caatatttga agagttattt tttagagaat atatatggaa ctatctaagt aattttataa
     7441 agtcaaagag taaaattatc tgtataacat ctatactatc tggaatttat aatataggat
     7501 acatagatgt tataagaaat tatgtaatat tgtataacaa ttcgagctac acctttgaag
     7561 ttattatttc aaaaattatg ataggtactg tttttggtat tgtacttgga ttagttaagt
     7621 ataggtttag agatgtaggg ttttgtatat tattgaggag cttatttgct atatttatta
     7681 ggtagatagt ataatcggtt tataaggttg ttttaaattt aattttaaat caaccttatt
     7741 ttatttgtgt tttataaagc acttatatat taataactaa taaatttaat aaaaattatt
     7801 tgaatatttt taaaatatat aaatatgtat tgacatataa atattatgaa ttacaattta
     7861 tataaactaa taaaaattaa ataatattaa tcaacttatt gaatggagga ataaaaatga
     7921 aatttgtgac tttttgtagt agcaatgaag aaaaaatagg tgtatttaat tcagaaacaa
     7981 atagtatata tgaaattaat agtttaggat taagtaaatt atatactgat atgaataatt
     8041 ttattgaaaa cgtatctaca ggagatttag aaaaaataaa aaacaattca tttgaaaacg
     8101 caaagtgtta taagttagaa gaagttaaat tatgttcccc aattgtaaga cctaaaaaag
     8161 atattatatg tttaggtcta aactataaag accatgtcaa tgaaatccca gatggagtta
     8221 taaaaaacgt agttatgcca gattatccaa tttatttttc taagagagca gacaaaataa
     8281 ttggagtgga tgataagata agcttacatg gagatttggt agagaagttg gactatgaat
     8341 cagaacttgc agtaatcata ggtaaagaag gtataaatat atcaaaagaa gatgcttatg
     8401 aatatatatt tggatataca atagtaaatg atataagtga aagagcactt caagataaac
     8461 atgttcaatg gtttagagga aaaagcttag acacacatac atctatggga ccttatatag
     8521 tacataaaga agaatttgaa catcctttaa aattagatat aagtagtgta gtaaatggag
     8581 aagtaagaca aaattctaat acagagtact ttatatttga tatacctact ataataaatg
     8641 atttatcaag aggaatgact ctaaagccag gagatataat ttcaactgga actccagcag
     8701 gggttgctat ggggatgaat cctcaagtat atttaaagca tggcgatata gttgaatgta
     8761 aagtcgaagg aataggagtt ttaaaaaata tagttgatta atggataaat atgccatata
     8821 attttattag tacgaataat ttaattaata taggattaat caaacttatg attaatttat
     8881 tatagagagg gatgagtata tggcaagaga actagaatta aagtatggct gtaatccaaa
     8941 tcaaaaacca tcaaagattt atatgaagaa tggagaactt cctatagaag tattaaatgg
     9001 aaaacctgga tatataaatt ttttagatgc atttaatagt tggcaacttg tgaaggaatt
     9061 aaaagaagct acaggtctcc cagcagcaac ttcatttaaa catgtaagtc cagcaggagc
     9121 agcagtaggg gtacctttat cagatacttt aaagcaaata tattttgttg atgatttaga
     9181 gctttctcca ttagcttgtg cttatgcaat ggcaagagga gcagatagaa tgtcttctta
     9241 tggcgatttt atagcacttt cagatgtatg tgataaggag actgcaacta ttatagcaag
     9301 agaggtttct gatggtataa tagctccagg atatacagat gaagcccttg aaatattaaa
     9361 aggaaagcgt aaaggcaact ataatattgt aaaaatagat gaaaattata ctcctgaacc
     9421 tattgaaaca aaggatgttt atggaattac ttttgaacaa ggtagaaatg aaatattaat
     9481 aaatgaagac ctattaaaag atattccaac tgataataaa atttttacag atagtgcaaa
     9541 acgtgattta attatagctc ttattacatt aaaatataca caatcaaatt ctgtttgtta
     9601 tgctaaagat ggacaagtaa taggggtagg tgcaggtcaa caatcacgta ttcattgtac
     9661 tagacttgca ggtaacaaag ctgatacttg gtatcttcgt caacatccta aagtattaaa
     9721 cttaaaattt aaaaaggata taggtcgtcc agatagagat aacactattg atgtatatct
     9781 ttctgatgat tatatggatg tgcttgctga tggcatatgg caaaactttt ttgaagaaaa
     9841 accagagcct ctaactggag aaggaaaaag agcatggctt aaaactttaa caggagtagc
     9901 acttggttca gatgcatttt tcccatttgg agataatata gaacgtgcaa aaagaagtgg
     9961 ggtaagtttt atagcacaac caggtggttc tattcgtgat gataatgtta tattaacttg
    10021 taataagtat aacatagtca tggcatttac taaaaataga ttattccatc attaaaatac
    10081 tgaaagtata aagataatat agattaggta aaaatctaca acaagttttg ttgtagattt
    10141 ttttatttgc tgtataattt taaatagaac tttattttta gatattcaat ttggaggttg
    10201 atatggcttt aaatataaga aactatagcc tattatgtaa tcaaggaaca gctaaattta
    10261 atgaagatgt agtcggtata aatccatttg gggcatgggt tttagatggt gctacaggac
    10321 ttaatggaaa aaatttagtg tcaactgaaa gtgacgcaag gtggtatgtt cagtggtgga
    10381 ataggtattt atataaaaat ataaataaag aagaatcatt aaagagtatt attaatactg
    10441 gaataataga agtaaaaaaa gagtatgaac taagattaaa tgggtttaag gttgaaaaat
    10501 tagatttacc atctagttct atagttgttg taaaatttca tgaaaataaa gtagagtatt
    10561 ttttattagg agattgtgct ttgtttatta aaaatggaga ttccaaagtt ataaaagata
    10621 ggagtatttg taaatttgat aatactgtat ttgatgaaat gagtaaactt tctaacttgg
    10681 aagagatggc ttttgatgag attagaacta gtgttatgga tacaatagta agcaatagat
    10741 tgaagaaaaa tacaaaagag ggctattgga tattggattt tgatgaggaa gctgtggaaa
    10801 attctatata tggatacatt gaaataaaaa aagattttca attgatgcta acgagtgatg
    10861 gttttacaag tgcatgtgat agatataatc taataaaaga agaagaattg atacgtatag
    10921 cagaaaaatt tggtatagga tatatacata ataaagtaag ggattttgaa gataatgact
    10981 ataaagcggt taaagtgcca agatttaaag taaaagatga ttcatcttgt ttatatttag
    11041 atatatataa tgattagact gtataaaact atttaatttt agaaaggagg taagtgaaaa
    11101 gtgaaaattc ttcatataat aacacaaaaa ccaaatagta caggtagtgg tatatatttg
    11161 tctggtatga taaaagggtt cgaaaaaatc ggtcataagc aagctgtaat tgcaggtata
    11221 gatgtaaatg atgatgtaaa ttgttttcca agtgaagtaa gtttttatcc agtaaagtat
    11281 aattgtgggg agttaaattt tcctgttgta ggtatgagtg attctatgcc atatgaaagt
    11341 acaagatata aagatttaaa tatagacatg ataaatagat tgaaatatca atttaaagta
    11401 aatatagata aggctatgaa tgattttaaa ccagaactta taatatgtca tcatttgtac
    11461 ctcttaactg catttgttag ggaaatggta aaagatataa aggttatgtc tatttgtcat
    11521 ggaacttgtt taagacagtt aaataccatt gatttagaga aagaatacat aatagcaaat
    11581 ataaggaaat tagatttaat atttgctctt catgaaaatc agaaatatga tattataaag
    11641 acttttggag taagtgaaag taaagtagtt gtaattggta gtggttataa tgatgatata
    11701 ttttataata aaaattataa aataaaggat gataaaataa aaatagtatt tgcaggtaag
    11761 atatgtaaat caaagggact gattccattt ataaaagcta tatctaaatt aaaatatagt
    11821 aaagatttaa tagaagtcaa ttttgcaggt actggaagcg atatcgaatc atataatgag
    11881 atagttaaac tggcaagtaa gtctccattt aaaatgaatt tcctaggaaa gttagaacag
    11941 agagatttag ctgagctatt caacaggtca caaatatttg tgttaccatc attttatgag
    12001 ggattaccag ttgtagtttt agaagcttta tcttgtggca cagatgtaat cactactgat
    12061 attcttggtg ttaaagagtg gattggtagt gagattaata attctggaaa gatagaatat
    12121 atttctctac cttttatgga gaaagaaggt attccaaaag atgaagagtt atatgatttt
    12181 gaaaataatt tgtataatgc aatagattct aagatacaat ctttattgaa taactcaaat
    12241 aagaaaacat ctgtagatat gtcaaaaaaa acgtgggatg gcttagcata tagaataaat
    12301 gaagttattt taatggatga attgtgttta gtctaaaatt atttaaatta taaaaaagtg
    12361 taactatttt tacactattt ttaaatgatt ttatattata ctaatactaa atatatttgt
    12421 tttagggagg taatttatga ggattagtaa aaaggtattg gcgttaggta tttcagcatt
    12481 actgttaagt gttagttttc caacatcaat aaacgcacta gataagattg aaaatattca
    12541 aggagtagat aaatatgaaa cagctgggct tatttcagat aagcaagatt ttacaacagc
    12601 agttcttatc aatgcagatt ctacaatggt agatggaatt gctgcaagtg gtcttgcagg
    12661 agtaaataat gcagctattt tattaactaa taaggatgat ataccagaag caacgcttca
    12721 aagactgaat agagttacta agatatatgt aataggtgga gaaaattcta taagtaaaga
    12781 tgtagaaaaa atgttactta tgagaagaat gcaagttata agaattgatg gtgtagatag
    12841 agtagataca agttataaaa ttgcaggcga aatagaaaaa attaaaaaca gtgataagat
    12901 gtttttagta aatggattta aggatgaagc tgatgcagta agtgttgcat ctgttgctta
    12961 tagagatgga gctcctataa tattgacaaa agatatacct tcagcagaag aagacacaga
    13021 cttagaccca tggtttggag tttctcctgt tccagtatat gctattggag gagagtctac
    13081 attaagtgat tacatagtga gcagatatag agcaactaga attggcggtg ttgatagata
    13141 tcaaactaat aaaaatgtta ttgaaaagtt ctataatgga gcaaaagagt tctatataac
    13201 aagtggagac gatttagttt atgctttagt tgcatcacca cttgcaaaaa atgctcctgt
    13261 tgttttagtt tcaaataaaa gtgataagag tatattaagt ggagctagta aagtaacagc
    13321 aataggtata agtgataaga gtattataga gcaatgtttg gatgctgtaa agaagtaatg
    13381 gagtgttatt agtaaaatga atatacacat tgaaaattag tagattgttc attttataat
    13441 ggtataaaat atatgggaag aggctatcaa atggagataa gcctcttttt catttatttt
    13501 tttgcgtccc ttattggtaa taaaagcttc ccctgctaaa attcttcccc aaaatacggt
    13561 ttcttcttgg ttggtttttt ttacagccat ttcagatatt tttatcaatt cacagtttgc
    13621 ttcaaaaaat acggttttca accatgagtg aaattctgtc agagtatctt gtgtaacatc
    13681 atgatgacaa acttccgtag atattaccat attaaatatt ttcaattttg ggttcattcc
    13741 acctgctgtc cctgaattga gaacgatatt tacttcaaaa aggtcaatac tttagtttcc
    13801 aaaaaacggg gatttattgc tttcgtttac tttttcgatt tttccagaca ttgtataaaa
    13861 tatctttaag aattaacatt gcttccagtt cattatattg gtattcctgt tttaattcgt
    13921 ccaacatcat gttaacctta taagcataat tagaaatatt aacatctgtc tgatatttaa
    13981 gaagaatagg gtattttttt ccccaggcat ttgtccaatt gattttttct tcggtttcct
    14041 cacttgtttt attaatcgct tcctgaattt cttccatgtc aatatcaaaa tcaatcaatt
    14101 catctaatga cacattgtaa agtattgcta atttttttga ctgacgaatg tctgggactg
    14161 tttcatccgt ttcccatttt gaaacagtct gcctgcttac ccccagtttt tcagctacat
    14221 cttcctgtga aagcccatgt ttcttccttg cctgaaataa actatttcct aaattcattg
    14281 aaattcctcc ttatctgttt atgggattat tatattattt gataagaaca aactctacca
    14341 actaaaatta tcatatttgc tatttttatt aacataattc aaagacgtat ttattctgca
    14401 aattaaaatc tgctaacctc tgcgaatatc aatcattaac cacttttatc cattccctgt
    14461 ttttgtttgt acgtaaatat ctcttaattc tggtgtaaac tcctgtaaaa tcgcttcgac
    14521 aacctctttc tctttctttc aaacaacaac taaaacgtaa aataattttc tttcggatat
    14581 actgtgcata aattttttct aggtttttta aatttgatat tccagccaaa ttcccaactg
    14641 caagaactaa atatctattt tgagacacta tctccttttc ttatattata ttatttttta
    14701 tatggaataa atttcataag tattatcttc ctttaatttt tctaaccaaa ccatttctaa
    14761 aatatctagt tcttctaaat agtctttctc tgttttgtta tcatcatgtt ttataagctc
    14821 taaaacttca aatgtaaatg ctttttcacc atattttttc cattcattat ttaattcttt
    14881 tatcatacat gagccagcac caagctgaaa tttgagagag ttaaatttgc tttttatatc
    14941 attacttttt ccaaggtaaa tctttttagt aatatcattt tttattatga atacacctgt
    15001 gtcaaatttc atttctttgt ataattgttt taattcacgt tttctatcca tgattttaga
    15061 tacctctctt tataattgat atgtaatatg taagtgtaaa aatatattac atatcaatta
    15121 taaagagatt aattttatat gtaaatagtt tttataaatt taattatgaa ttagtcatca
    15181 ataaacaaga aatttagctg tagaaatcca ttataaaggc tattataaat tttaatatgc
    15241 taaaaatata aaaagaatag ttgacagtgt agaaaatatg gaataaacta aaagtgtaat
    15301 agtaatatta aattatatat tacattgaaa ggtgtgaatg attattatga ataaaaaaat
    15361 agaattaata gggaattttg caagtgattg gattaggtat agtgattatg aatataaaat
    15421 tactgaagaa gatgaattat atatagttcc aacagaagat gctgtgttta ccatgtataa
    15481 cccatttgat gtagcagatg atataatagt agatataatt cgcataggac aagaggcatt
    15541 acaagatata tcaaaagaga caaaagaaat gaaactaaag aaagaaatac ttgaatttgt
    15601 aaaaaagtat ggattattgg gtttaatata ttcaagtgta tataatagag atatagttgg
    15661 agaagaaaga gtactgatga ttgaaaagaa ttatattaca aaagagaata tattgagtac
    15721 agatgaatat gtaaacatgt ttataccatt tgcaagtgat gaagatgtgt attttaaaaa
    15781 gtatagaagg ggtatagatg tatcaaagag agaagaatct ccaaagtttt ttggaaagag
    15841 accattgatt ttagatttag tattttcaaa gttctacact gaacaactag attggattgt
    15901 aagttttgct aaagatatgt gtaagcattt taatcaaatt tgtgtatata aaaatacatc
    15961 taatctatta actgaaagtg ttactattat gtctggaggg tttaatcctc aaaagatagg
    16021 attttctata agccaattag ataaaccaat tattgcatgg gagtttgatt ctttgaaaac
    16081 agctattgag actatctatg cctttgcaat tacaagtgaa ccttcttctt taaataaatg
    16141 caagtactgt ggtaaaattt atattcctac aaattctaaa tctcaatact gttcaccttc
    16201 ttgtagaaat tgctcaaatg taaagaaaag tagaagtaga gccaaaaaag ataaataaaa
    16261 ttaataaata tatcattgtt tagattttag taaataacta ctgaattaaa agttgttata
    16321 taaaattata tatctaaatg atggtatttt ttttgttgag aaaattgaaa agtttaattg
    16381 agatagtaat tttgagttga caaaaaccac ttttaacact actattataa gtggttggta
    16441 aaataatata tactaattca aatatagcat aaaaaacaaa aaaagtcaat ggaaaaaata
    16501 aaaaaggaga atttaaaaat gatatcacct attattttga gttctataaa tcagaattta
    16561 aaggaaatag aaagaaatga actgcttgaa actaatatag aatctggaga ttatggtcta
    16621 gctctttctg aatctgatgt aaaagatata ataaattcga gagataatac tttaaagggg
    16681 tatggaagaa tagaattaga tataaaagtt acaaaacagt taatagaaaa tatatataca
    16741 tcacaatata caaatgtgga caattattta gaggctatta atgatatgca agaaattttt
    16801 tattatttaa aaaatgaaac tgatgacaag atatgtgatg atgagattat agaaatactg
    16861 ggtgattttt atgaaaaatt ttcaggcaat atggacaatg taagaggaga agcagacgaa
    16921 tttgcaaaga aatttaaatt tggagaagtt taatatatga taggagagat tttgtatgtt
    16981 aaataattta agtaatatta atataaacga aaataattta ataaagaacc aatattccat
    17041 atctctactt aaggaatgtc taaattgtaa agtgatagat gaaagagaag tatataatat
    17101 acaacaagaa atatctctaa tacttatgga tttgattaaa aaatatacaa atggtcaaag
    17161 cacatcagta aaaacagaag ttgccgaaaa actgttgatt tcaatatggt atgctattga
    17221 tgcatatata aataagcttg gagatataga aaaaagagtt gtggagttga ctgctaagaa
    17281 tgttaaaaaa gtgtaccttg gaggaattga aatattagag gaagagttag ttaggctaaa
    17341 agatttttat aaactaatga tgagtagaaa attggatact aattcaatag catataatga
    17401 taccttgatg gagttatcaa gtttctttaa aagttatatt gtaaaatttg aagctcagga
    17461 cgttccagta agtatagatt atccattggc actagatgat atggatgtta agggtattta
    17521 ttatgtgaag aattatattg agagtataga tatagaaaat agattttgta acctatttaa
    17581 gaaaaaagat agagaaaaat tattttatga ctatggggtg acttataaaa tagattgtga
    17641 tgttatactt gtaaatttat ttgaacttat tatgaataat tgtatttttt ctactatttt
    17701 aggaaatgaa gtgactaatt tagaaatttc aaaatatgag tatgaatttt tagaaaaaca
    17761 atttaaaaat atgtacaata atattactga agataaagtt atagactctg aagaaagtaa
    17821 tataattgaa ttgattgaag tagatgatag ttttaacact gaagtagata gtaatttgga
    17881 ttataatgat tttgaagaaa ttaacagaga aattagaata aataaaaaaa taactttact
    17941 acttatggaa gcaattgaag tattaataaa aagattaagc atagaagatg aagatgtaat
    18001 aagatatatt aaaaaatatg aaaagatatt tattgaatct gtgctaagct caataaaaag
    18061 taacactttt aaaagtatgg taactgtgag taaaaataaa aatgaacact tagaagatta
    18121 tattgttgat gatgaaagta aattggatga tgaatcgttt agagagatat tcaatgagat
    18181 attagatagt acatctataa ttgaaaaaat aagaataata aaggaaaata tcaatgctaa
    18241 aaaagacttt atcgatattc ttgaatctga atgtttattt ggagaagagt atttaatgtt
    18301 gtttgcatca ttaagtgaat tagaattggc aatattgggt agtgtagtat tttatgaaga
    18361 tattagaatg agagaaatta atatattaga atttatgtta aatgagaaaa ctgaaactac
    18421 tttatggaag atagagtaca ttaaatttat gaagaggcaa agtgaagata agatatactc
    18481 catagaagag catatgaata aaattaatta acaaaaaatt gtgaataaat agaatgttag
    18541 tatataatat aatctgtaaa gcatatcaaa aatacatata tttggaggaa aattatgaaa
    18601 gagtatatca taaataatgt tgaagaaata agagaagaat taattgattt atctaagaaa
    18661 atatgggaaa atccagaatt agcctttgaa gaaaagtatg caagtagcat tcaaaaagaa
    18721 tacctaaagt caaaaggatt taaaattgaa gaagtagaaa atttacctac tggatttata
    18781 gcaagttttg gagagggtaa acctgtgatt ggcatacttg gagaatatga tgctttacca
    18841 gaactatcac aatgtgtaag tgcagaaaga aagccattgg tagaaggaaa agctggacat
    18901 ggatgtgggc ataacctgtt gggtgtagca ggtgtaggag cagttgtttc tataaaaaaa
    18961 ttaatagaag aaaataaatt taatggaaca ataaaatatt ttggttgtcc tgctgaagaa
    19021 gaaggtggag gaaagacagt tatgtgtata aatggctgtt ttgatgatgt agattgtgca
    19081 tttacatggc atccttttga tataaatgca ccttggagag gaggaagtct ggcaaactta
    19141 tctgtaaaat ttaaatttaa agggataaca gcccatgcag ctcaagctcc tcataatggt
    19201 agaagcgcac ttgatgctgt tgaaattatg aatgttggag ctaattattt gagagaacat
    19261 gttattgact ctataagaat gcactatgtt ataactaatg gtggtggaag acctaatgta
    19321 gttcctggat ttgctgaaag ttggtacttt attagaggta aaaaagcaaa agatgcagaa
    19381 cacgttcttg atagattaat aaaagtagct caaggagcag caatgatgac agaaactgaa
    19441 atggagtata aggttacaga tggaatatat gactatatac caaaccaatg tttgactgat
    19501 ttagtttatg acaatatggt ttttgttggt tgccctaaaa atactcctga agaagaagaa
    19561 tttgcaaaaa agttgtgtga tactcttact agagaagaaa gattaggtgt agcaacatca
    19621 ttacaaaatg ataaatctat tgtagaaagc tatatacaca aggaaatagt tgacggtgac
    19681 aaagataaag gactagctgg ttctactgat gttggagatg taagttatgt aataccagtg
    19741 gctcaatttg caatggcagc ttggcctgtt ggaattgcaa gtcatacatg gcagtcatgt
    19801 tcatcagcag gttcaaatat aggattttct gctatgataa attcagctaa agtactggct
    19861 tgtagtgctt atgatgtatt tatggatact aaaattattg atgaagcaaa aattgaattt
    19921 gataaatcat tagatggaca aaaatttaaa ccattggtat aattttggta ataaaatatt
    19981 tcagggggga atagctatga gagaaaaagt tgaaaaagtg cttgaggaaa agataaaacc
    20041 agtattacaa agagatggtg gagatgtaga gcttatagat gtaaatgaaa atggtgtggt
    20101 tttagttaga ctacaagggg catgtagtgg gtgtccagga gcgactatga caatcaaagc
    20161 tattattgaa aatgttttgg tgagtgaagt tccaggagta actcaagtat taggtgttta
    20221 atagttagcg tttattaaat gtatgttata cataagtatt taataaagaa gtaaaaaaaa
    20281 tagtgaaaca atatatatga aaaagtatga gaaagataat aaaacattat agaaataatg
    20341 aaatatttca t
//
LOCUS       DAELLV010000045        20047 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.045, whole genome shotgun sequence.
ACCESSION   DAELLV010000045 DAELLV010000000
VERSION     DAELLV010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20047
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.045"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..661
                     /locus_tag="KPD58_001390"
     CDS             <1..661
                     /locus_tag="KPD58_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0265168.1"
                     /translation="GDIKFQFKYGFYFLYLFLSIIYICIINVFPTFMREKIAIIMIYS
                     DPAAMGLFFMGAIVLLEKSQRVLNSLAVSPVKVSEYILSKVISLGVISSIVAMFIAIT
                     LNLDNIIISTIGTFFSSIIFSLLGLIIASKASSLNQFIVLSIPIEIICFIPPILNVLL
                     DTKSYANLYPFNICISLISGDKNFIMINILILISIIIIIYFITYYFICSSWKKVGGVK
                     L"
     gene            658..1374
                     /locus_tag="KPD58_001391"
     CDS             658..1374
                     /locus_tag="KPD58_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0265169.1"
                     /translation="MIKIINAFKQEFEQIKRDAMLFIVCVSPILCGVFIKFGIPLIQN
                     ISLNKFYYQLNLEPYFLMFDLLLAFITPFMFFFASTMVILGEIDDSISRYLIVTPLGK
                     TGYLISRFGIPGILAFIITMILLIFFSLTKISLLLNLSISLLVLLQGVIISILVISLS
                     SNKLEGMVITKFSGVFMMGILAPFFILNKAQYILFFLPTFWISKAFKEDNYVYMFISI
                     VISLIWILLLFKKFSKKIVK"
     gene            1471..1734
                     /locus_tag="KPD58_001392"
     CDS             1471..1734
                     /locus_tag="KPD58_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0265170.1"
                     /translation="MKNKIKILREKLGLTQEQLGRLVGTSRQAINAIETGKNEPSIWL
                     AYDISRIFNEPIESVFLFEESERKSRAQISRGEYYGYKKDKNI"
     gene            1706..2161
                     /gene="def"
                     /locus_tag="KPD58_001393"
     CDS             1706..2161
                     /gene="def"
                     /locus_tag="KPD58_001393"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBG0265171.1"
                     /translation="MAIRKIRTFDDEILRKKSKYVENVDNKIREILNDMAETMYNTPN
                     GGGLAACQVGVLKRLVVIDLGEGLIKLVNPEIIEQEGEQIVVEGCLSFPEVWGKLKRP
                     KKVTVQALNEYGEKIEIKGSGFMAKCLCHEIDHLNGIVFTDKIIEHVKL"
     gene            2213..2674
                     /locus_tag="KPD58_001394"
     CDS             2213..2674
                     /locus_tag="KPD58_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HBG0265172.1"
                     /translation="MRLWHKDLIDVLPKNQLVSQWRELLAIKGSIDKKGTPNHLLVNK
                     VLNYSIDEFKFYTKIVHDEMLKRNYKPNELKYTSILKWKNRNFANDISNEHSLNLENL
                     YDDWHNKMYLKQCLYNLEEKATCGGIPINEWNILLCKYSKDYELWSGNIMF"
     gene            2724..3626
                     /locus_tag="KPD58_001395"
     CDS             2724..3626
                     /locus_tag="KPD58_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG0265173.1"
                     /translation="MYNKIKYIPINCEIACNKLKRSMPYKWDLNIYRGCEHGCKYCYA
                     IYSHKYINSNNYFEEIYVKTNIVEKLERQLKSSKWKKEVINIGGVTDSYQPIEADYKI
                     MPEILNLLIKYKTPAIISTKSDLILRDYDLIDKLSRITYINVASTITTVNEDTQRLIE
                     PNGVDSMRRFKMLKEFRKTNASVGLHIMPIIPYITDDFDNINSLFRHAKECNVHYVLP
                     GTLYLRGITRGVFFDFVKKEFPELFDKLSILYSAGSLNREYKNQLYKMVNELRYKYSL
                     SSSYSKIMKEKLKNSGDVQISFFD"
     gene            3996..4808
                     /locus_tag="KPD58_001396"
     CDS             3996..4808
                     /locus_tag="KPD58_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG0265174.1"
                     /translation="MYLERPELYKQSEINFWDDEYISKQLLKAHLDTNFEGASRNFNF
                     IEDSVNWIVTVANPANYPKLLDLGCGPGLYAEKFAQKGYKVTGIDFSKRSINYAQNRN
                     KETNLNINYLFQSYLNMNYNEEFDLATLIYCDYGALSTENRRLLMEKIYDSLKPGGKL
                     ILDVFTINKYNNFEEIKYWEINEDGGFWSNEKYMCLQDNCKYNDYNTLEQTLVITEKD
                     ENVFYVWNHYFSKESFLLEVENIGFKSVEFFSNVKGEAYSDDSMTMGLVLQK"
     gene            5856..6617
                     /locus_tag="KPD58_001397"
     CDS             5856..6617
                     /locus_tag="KPD58_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896901.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBG0265175.1"
                     /translation="MIGIYFSGTGNSRYCVEKFLQEYDITANSYSIEDNELLQHINNH
                     ENIIFSYPVQYSNVPKILKDFIINHSTLWNGKRVFIIATMALFSGDGSGVLSRILKKY
                     GAITVGGLHVKMPDSIGDEKVLKHSLEYNRKLVIKAEKKVTKAAKKLKHGSPPKEGLG
                     FLSHIVGLFGQRLYFINKTNMYTDKLKINTEECIVCGKCVNLCPMKNLVIKNCMIVAN
                     GQCTMCYRCVNNCAKKAITLLGKKVVEQSNIKKYM"
     gene            6682..7464
                     /locus_tag="KPD58_001398"
     CDS             6682..7464
                     /locus_tag="KPD58_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBG0265176.1"
                     /translation="MEYYIVDSFATKLFKGNPAGVCVLDRRIPLELMQKIAEENNLPE
                     TAFVVKGKGNYELRWFTPKAEIDLCGHATLAAAYVISNFIDVNVKRIDFFTQSGKLEV
                     TRNGNLYEMIFPEIMPIEIELSPQQANLIGCVPSAVYSSRDLILLLNSEQEVINYKPN
                     YAQLRKLTDWLGIIITAQGSNTDFVSRYFCPELDSEDPVTGSSHCNLIPYWSEKLGKH
                     KMVAAQLSNRGGIIQCEVLKDNTVKISGEAVLFMQGTIKIDI"
     gene            7731..8150
                     /locus_tag="KPD58_001399"
     CDS             7731..8150
                     /locus_tag="KPD58_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HBG0265177.1"
                     /translation="MDKISGKLTVLFEEPFWVGIFERQDGKKYEACRVVFGAEPKEVE
                     VYEFILERFFSLDFGSIKLEKNVTKDNIGYKRMQRKVKKEQEKETIGTKAQNALKLQY
                     EERKQDRKNLAKNRKEEEKERLFNLKQEKRKAKHKGH"
     gene            complement(8253..8519)
                     /locus_tag="KPD58_001400"
     CDS             complement(8253..8519)
                     /locus_tag="KPD58_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HBG0265178.1"
                     /translation="MKNFILIYFIVINSIAFFSMYIDKKRAIRNEWRIKEATLMSIAV
                     IGGSIGSIIGMYSFRHKTKHIKFTFGIPFILFLQFLLVYFYILK"
     gene            8746..9447
                     /locus_tag="KPD58_001401"
     CDS             8746..9447
                     /locus_tag="KPD58_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265179.1"
                     /translation="MKFIVEKEVFDKLENVCFGVVVAKGIDNTKEIERISNLLDISID
                     RVEDYFKDKKVKESEEIIPYREAFRSLGMNPNKFMSSIEAMTTRVAKNKKLPHINPIV
                     DLGNSISLKYLLPMGAHDMDFRNDDVYVRFSKKGDKFVPFGETDVELMEEGELIYSVG
                     DMVKTRRWIWRQGEEGKITNSSKNIFFPIDGFTDANLDKVMSAREELAKLLKEIFNCE
                     IKVGFVDKDNPEMEI"
     gene            9529..10137
                     /locus_tag="KPD58_001402"
     CDS             9529..10137
                     /locus_tag="KPD58_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG0265180.1"
                     /translation="MKKTIIKNVNPMIENKFIGLFEIEYKNKLDEDKVWMVASRKSSE
                     QLKNIYLKNEEDSVDAVVIVGLHKSSKKLILIRQFRVPINGYIYELPAGLVDEGESID
                     ISVERELREETGLTLLEIKKNKSIDKVYLSPGMSDESVAFVYCICDGDITDEFLEPDE
                     EIEALLISQEDAKEILQSNHKIDTKAFLILQMFVSLGAKLFE"
     gene            10461..10925
                     /locus_tag="KPD58_001403"
     CDS             10461..10925
                     /locus_tag="KPD58_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4624 domain-containing lipoprotein"
                     /protein_id="HBG0265181.1"
                     /translation="MKKLVIILISIISMVSLTACKKSNESKYNNLNNKESGQTTIEMD
                     LDKNYDTSDPFVNARLFCVSNDIDVLDTEISFKMDGDSGIVEIKDNKTDETLWSNTWH
                     GSVDNDTFTISLSNIQKEKEYAICFTGIKINHAVVKVTFESNLVKEKEKPSK"
     gene            11562..12578
                     /gene="gap"
                     /locus_tag="KPD58_001404"
     CDS             11562..12578
                     /gene="gap"
                     /locus_tag="KPD58_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBG0265182.1"
                     /translation="MKIKVGMNGFGRIGRAVLRIAQEELGDNIEIVAINARATTESLA
                     HLFTYDSCYGTFRGEVEAKDEDTLVVNSKEIKILRYNDPEELPWKELGVDIVIESTGL
                     FTQREKAEKHIKAGAKKVIITAPGKNEDITIVIGVNEKEYDNEKHNIISNASCTTNCL
                     APFAKVLDEKFGIVKGLMTTVHSYTNDQRILDKSHKDLRRARAAAESIIPTTTGAAKA
                     VSRVLPQLEGKLNGFSLRVPTPTVSLVDLVCELKENVTSEQVNSVLKDAAEGELKGVL
                     GYCDKPLVSIDYKGDSRSSIIDALSTMVIEDNMVKVVSWYDNEWGYSSRTVDLVKYVA
                     EKLK"
     gene            12973..14142
                     /locus_tag="KPD58_001405"
     CDS             12973..14142
                     /locus_tag="KPD58_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265183.1"
                     /translation="MEKLTDRRQVKAVSMETFIFLILLVVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLETVFLIMAMAVLAGALSALLSEFGVIALINKIFAVFMKPLYGLPGASIA
                     GAITTYLSDNPAIIPFAKDKTFTQYFKQYQVPALCNLGTAFGMGLILTTFMISQGTEY
                     VLPALIGNLGAIIGSVISVRIMLTFTKKFYKYNPEEDKATGTLEKKDEFREIREGNVF
                     QRALDAILEGGKMGVDMGMAIIPGVLVVCTLVMLLTFGPSTDPVTGQEVYTGAAYEGI
                     KLLPVIGDKLGFILEPLFGFTSPEAIAFPITSLGAVGAAMSLVPEFIKSGAITPNDIA
                     VFTAMGMCWSGYLSTHIGMMDALNARQLAGKAILSHTIGGLCAGAAAHFIFTLVG"
     gene            14522..18775
                     /locus_tag="KPD58_001406"
     CDS             14522..18775
                     /locus_tag="KPD58_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HBG0265184.1"
                     /translation="MDELFHIGIDVGSTTVKVVVLNNSNNIVHKEYRRHYSDVKKSVK
                     EVLNGIYEKLGDINTTIIITGSGGIGISKKLGVKFVQEVISSTKSIEYFHPETDVVIE
                     LGGEDAKITYLSGGIDQRMNGICAGGTGAFIDQMASLLKTDASGLNELAKGYNVIYPI
                     ASRCGVFAKTDIQPLINDGANQTDIAMSIFNAVVVQTVSVLSCGRKIEGNVAFLGGPL
                     YFLSELREAFKRVLDLKDENIIFPENAQLYIAIGAGLLSVDEDNIQLKSLIEKLDSIK
                     NIEDGEVNLLEPLFKDKNEYEEFSRRHEKEKINYVDINSIKSNCYLGIDAGSTTTKAA
                     LIDEDGRLVYSYYNSNEGNPLKTTIKVINEVYDILPKNIKILSSTVTGYGEGLIKKAL
                     KIDNGEIETIAHYKAAKFFNKDVDFILDIGGQDMKCLKIKDGVIDNIILNEACSSGCG
                     SFLETFASSLSMTIEEFAHEGIYSQNPVDLGSRCTVFMNSRVKQAQKEGASVGDISAG
                     LSYSVIKNALFKVIKIRDLNEIGNNIVVQGGTFYNDLVLRSFEKLIGKNVIRPNISGI
                     MGAFGSALIAKEKYESGYKTSLLSKEELNNIKLEASVTRCKGCSNHCLLTINKFSDNE
                     IFISGNRCEKGEAIYGDKKIGLEKKKPINLFKYKYNRIFRYKPLEKEEAKNGEIGIPR
                     VLNMYEDYPFWFTFFNAMGFRVVLSDRSSKQLYETGITSIASETVCYPGKLVHGHIEN
                     LIAKGIKNIFYPSVTNENKEDSNADNYYNCPVVISYSEVIKNNVENIRNKNINYINPF
                     ISLNDKEKLKKRLYDELSRHFSDIKITKEEVNHAVDKATDEQNLFKMEIQAAGERALK
                     EIKERNMKGIVLCGRPYHIDPEINHGMPELINSLDMAVLTEDSICHLANVQRPLRVVD
                     QWVYHSRLYKAASFVRDKNYLELVQLNSFGCGLDAVTTDQVQEILNEKSKIYTIIKID
                     EGNNLGAAKIRMRSLKAAMFERESKNIDVKNIKVNRIQYAKNNQITNKHTILAPQMSP
                     IHFQFLEKAVNLSGYNIEILKDTDSSVIEEGLRYVNNDACYPAIIVVGQLITALKSGK
                     YDLNNTSVTITQTGGGCRATNYIGFLRKAIYDAGFKDIPVIALSVNGIEDSGIMDNIS
                     LKLINRLFMSVVYGDLLMKVLYRVRPYEKVTGSANALYEKWVDICKSSLVKAKISVFT
                     RNIKDIVKEFDNLEILDIKKPKVGLVGEILVKFHPIANNNLVDILEREGAEAVVPDLT
                     NFFLSCAFNTIYKHTHLEGSRKSRMIGEAFIYITGIYQRVYKKALDKSERFYAPANIK
                     NVAKSTEPVVSLGNQTGEGWLLTGEMVELLNEGVENIICMQPFGCLPNHIIGKGSIKE
                     LKRLYKNANIIPIDYDPSASEVNQLNRIKLMLSKAFKNI"
     gene            18924..19418
                     /locus_tag="KPD58_001407"
     CDS             18924..19418
                     /locus_tag="KPD58_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBG0265185.1"
                     /translation="MSLVYDVYKSKLGNLYILSEDGFIVSIYIGDEKFNNLKNECIKK
                     GIDIKRSKEDLKEAISQIDDYFNGKRKVFNLNIRIEGTEFQKSVYREMLKIPYGETLC
                     YSDIARNINNPKSVRAIGQASKSNKIPIIIPCHRVVGKKDIGGYMGNHSDLKEILLNL
                     ERQS"
     gene            19534..19911
                     /locus_tag="KPD58_001408"
     CDS             19534..19911
                     /locus_tag="KPD58_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HBG0265186.1"
                     /translation="MCDKVIASKYHEKGFNCAESVIKAYNEEFNTDIPVCLGSGLGSG
                     CGVASLCGAVNASNIIIGYVKGRNHEDESTKAKVYAKDLTTTVRKEYGSELCIDLKKD
                     LVACREIMDFAYDSLKETLKKEL"
BASE COUNT         8081 a         1943 c         3441 g         6582 t
ORIGIN      
        1 aggagatata aagtttcaat ttaagtatgg tttttatttt ttatatcttt ttttaagtat
       61 tatatatatt tgtattatca atgtatttcc aacttttatg agagaaaaaa tagctataat
      121 tatgatttat tctgacccag cagcaatggg attgtttttt atgggagcta ttgtacttct
      181 tgagaaaagt caaagggttt taaattcctt agctgtatct ccagtaaaag tatctgagta
      241 tattttatca aaggtaattt cattaggagt aatatctagt attgtagcta tgtttattgc
      301 tattacatta aatttagata atattataat atcaactata ggtacctttt tttcttctat
      361 tatattttct ttgcttggat tgataattgc aagtaaagca tctagtttga atcagtttat
      421 tgttttaagt attcctattg agattatatg ctttattcca ccaatactga atgtacttct
      481 tgacacaaaa tcatatgcaa atttgtaccc ttttaatata tgtatttctc ttattagtgg
      541 agataaaaat tttataatga ttaatatttt aattttaata tcaattataa taataattta
      601 ttttataact tattatttta tttgtagcag ttggaaaaag gtaggtggag taaaactatg
      661 ataaagatta taaatgcgtt taaacaagag tttgaacaaa ttaagagaga tgctatgctt
      721 tttatagtat gtgtttctcc aatattatgt ggagtattta taaaatttgg aataccatta
      781 atacagaata tatcacttaa caagttttat tatcaattaa atttagagcc gtatttcttg
      841 atgtttgatt tacttttagc ttttattaca ccttttatgt tcttttttgc ttctacaatg
      901 gtcatattgg gagaaattga tgacagcatt tcaagatatt taatagtaac tccgcttggt
      961 aaaactggtt atcttatttc acgatttgga ataccaggta tcttagcatt tattattact
     1021 atgattctac ttatcttttt ttctctaact aaaatttctc tcttgttaaa cttatcaatt
     1081 tctttattag ttttattaca aggagttatt atatcaatat tagtgatttc tttatcatca
     1141 aataaactag aaggaatggt aattacgaaa ttctcaggtg tttttatgat gggtatttta
     1201 gcaccatttt ttattttaaa taaagcacaa tatattctgt tcttcttacc aactttttgg
     1261 atatcgaagg catttaagga agataattat gtctatatgt ttatttcaat tgttatatca
     1321 ttaatatgga ttttattatt atttaaaaag tttagtaaaa aaatagttaa ataagaaaat
     1381 ttaatatata aatgtaaggt tgacttgaca gtaatgttga aaaatgttac atttaagatg
     1441 taaagttaac cttacatgaa tggagcaaat atgaaaaata aaataaagat attaagagaa
     1501 aaattaggat tgacacaaga acaactaggg agattagttg gaacatctag acaggctata
     1561 aatgctatag aaactggtaa aaatgaacct tcaatatggt tggcatatga tatttcaaga
     1621 atttttaatg aaccaattga aagtgtattt ttatttgaag aaagtgaaag aaaatcaaga
     1681 gcacagataa gtaggggaga atattatggc tataagaaag ataagaacat ttgatgatga
     1741 aattttaaga aaaaagagta agtatgtaga aaacgtagat aataagataa gagaaatatt
     1801 aaatgacatg gcagagacca tgtacaatac tccaaatgga ggaggattag ctgcatgcca
     1861 agtaggagta ttaaagcgat tagttgttat agatttagga gaaggactta ttaagcttgt
     1921 taatcctgaa attattgaac aagaagggga acaaattgtt gttgaaggat gtttaagttt
     1981 tcctgaagta tggggtaaat taaaaagacc aaaaaaagtt acagtgcaag ctctaaacga
     2041 atacggtgag aaaatagaaa ttaaaggttc aggttttatg gcaaaatgtc tttgtcatga
     2101 gatagaccat ttaaatggta tagtttttac agataaaatt attgaacatg taaaattgta
     2161 agatagattg gttattgaat ttataaaata ttttatagta aggagttaaa ttatgagatt
     2221 atggcataag gatttaattg atgtattacc taaaaatcaa ttagtaagtc aatggagaga
     2281 gctacttgct ataaaaggtt ctatagataa aaaaggtact ccaaaccatc tattagtgaa
     2341 taaagtattg aattatagta tagatgaatt taaattttat acaaaaattg tacatgatga
     2401 aatgttaaaa agaaattata aacctaatga acttaaatat acaagtatat taaaatggaa
     2461 aaatagaaat tttgcaaatg atatatctaa tgaacatagt ttaaatttag aaaatttata
     2521 tgatgattgg cataataaaa tgtatttaaa gcaatgctta tataatttag aagaaaaagc
     2581 aacatgtgga ggaataccaa taaatgaatg gaatatatta ctttgtaaat atagtaaaga
     2641 ttatgaattg tggtctggaa atatcatgtt ttaattacta tagattaaca ttatagtatt
     2701 attgtatata aagaggtgat tatttgtata ataaaataaa atatattcca ataaattgtg
     2761 aaatagcttg taataaatta aaaaggagta tgccatataa atgggattta aatatttatc
     2821 gtggatgtga gcatggatgt aaatattgtt atgctattta ctctcataag tacatcaact
     2881 ccaataatta ttttgaagag atttatgtaa aaacaaatat agtagaaaaa cttgagagac
     2941 aacttaaaag cagtaaatgg aagaaagaag taatcaatat aggaggagtt acagatagtt
     3001 atcaaccaat agaagctgac tataaaatta tgccagaaat attaaacctg ctaataaaat
     3061 acaagacacc agctattatt tctacaaagt cagatttaat tttaagagat tacgatttaa
     3121 ttgataagct atctaggatt acatatatca atgtagcatc tacaattacc acagtaaatg
     3181 aagatacaca aaggcttatt gagccaaatg gtgtggattc aatgagacga tttaaaatgt
     3241 taaaagaatt tagaaaaact aacgcatcag ttggtctaca tattatgcca attataccat
     3301 atattacaga tgattttgat aatataaatt cgctatttag acatgcaaaa gaatgtaatg
     3361 tacattatgt tttacctggg actttatact taaggggtat aactagaggt gttttttttg
     3421 actttgttaa aaaagagttt cctgaattat ttgataaatt atcaatacta tatagtgcag
     3481 gttcacttaa tagggagtat aaaaatcaat tgtataaaat ggttaatgaa cttagatata
     3541 agtattctct atcaagcagt tactctaaaa taatgaagga aaagttaaaa aactcaggag
     3601 atgtacagat atcttttttt gattagtttt atataaaaaa atttttttta ctatgatgat
     3661 taaaatttat taaaattaaa atttattgac aaatataaaa tttaaatata taattaattc
     3721 ataaaataaa aatatgaaag gatgatgttg ttcggtgata tttgatatca taaaatcgtt
     3781 gatataagta ttgctggaaa agagtgaata aaatcaaatt taataagcaa acaaagtaaa
     3841 gctatagttt tattgttgtg cttaaaaata tttggttcta ttttactctt atttaaactc
     3901 atctttttga agttaaacca cagagtaaac tctgtggttt ttttatttta aaaataaatt
     3961 ttaaggagtg gtatgttttg ttaaacaaat tatgtatgta tttagaaaga ccagagcttt
     4021 ataaacaaag tgaaattaat ttttgggatg atgagtatat ttcaaagcaa ttattaaaag
     4081 cacacttaga cactaatttt gaaggagcaa gtagaaattt taactttatc gaggattctg
     4141 taaattggat agttacagtt gcaaatccag ctaattatcc taaattactt gatttaggtt
     4201 gtggtccagg tttatatgca gagaaatttg cacaaaaagg ttataaggta actggaattg
     4261 atttctcaaa acgttctatt aactatgcac aaaatagaaa taaggagact aatttaaaca
     4321 taaattactt gttccaaagt tatttaaata tgaactataa tgaggaattt gatttggcta
     4381 ctttaattta ttgtgattat ggagctttgt caactgaaaa tagaaggtta ttaatggaaa
     4441 aaatttatga tagtttaaaa cctggaggaa aattaatatt agatgtattt acaataaata
     4501 aatacaataa ttttgaggaa attaaatatt gggaaataaa tgaagatgga ggattttgga
     4561 gtaatgaaaa atatatgtgt ctacaagata attgtaaata caatgattat aatacattag
     4621 aacaaactct tgtaataacc gaaaaagatg aaaatgtatt ttatgtttgg aatcactatt
     4681 tttcaaaaga aagtttctta ttagaagttg aaaatattgg atttaagtct gttgagtttt
     4741 ttagtaatgt taagggtgaa gcatattctg acgatagtat gactatggga ttagttttac
     4801 aaaaataata atttttttaa aatgaataaa taaaaacttg aaacctttaa caataaaata
     4861 acataataaa agcttacata ttgaaaaatg gaaactttta tgttattttt agtacattac
     4921 aaatttgctt atataaaaat caatacttga attattattt ttatgtgaaa tttatctata
     4981 gtatattttt ataaacaagt aatgttatta aaattaagta tgtagttaaa agtgtatagc
     5041 aaaaaggctg ttatattaat gttagaggtt cagggaaaat atataaagca aagtatttac
     5101 agataatgta gattgcacag ctataagtta gataatagaa ttatgtaatc aagaatttac
     5161 aacaaagtat aagataagga taatgctaaa ttgccataca ggtgctagat gtgtaaatgg
     5221 atttaattaa tactattgat aaagtgcaac aagcacataa accaattgat gagattatta
     5281 aaactataag agatatagtt gacataatta aaatattgga acttgtttat aattttaaat
     5341 caaatcaata atgattaaga ttaaaataat attaaggcat caatgttata agagtacaat
     5401 ataaggagta ctatatagta ctccttactt atacatatta ggcaatagga aattttttca
     5461 tagttaaatt ttcaagtatt ttttaggaaa ctttataatt gagaattaag ggtatagtat
     5521 gtgatgaact tactgaattt ataattaggt tatataatgg aataatcatc aatagtttta
     5581 ctttagggaa aatatatgct taaggaagta tagcttacta atattattaa aatgtatatt
     5641 taaaatgata aaaaaagaat tagacaagta tttagtaaat tgactatgca aaattctatc
     5701 aagcaggaac cctatgactt tagtcatggg aggttcagta ggtaacttgt tgaattaagt
     5761 ttatattatt taaataaact acttaattta gataatataa aaagtcagaa ttttaagaat
     5821 agagctatat aatcttagct attaggagaa ttactatgat aggaatatat tttagtggaa
     5881 caggaaattc aagatactgt gtagaaaaat ttttacaaga atatgatata acagcaaatt
     5941 cttattctat tgaagataat gaacttttac agcacatcaa taatcatgag aatattattt
     6001 ttagttatcc ggtgcaatat agtaatgtgc caaaaatatt gaaagatttt ataataaatc
     6061 attcaacttt gtggaatggt aaacgtgttt ttattattgc aactatggct ctatttagtg
     6121 gagatggctc tggtgtgtta tctagaatat taaaaaaata cggtgctata actgttggtg
     6181 gattacatgt gaagatgcca gatagtatag gagatgaaaa ggtattaaaa cattctttag
     6241 agtataatag aaagcttgta ataaaagcag aaaagaaagt aactaaagct gctaaaaaac
     6301 tgaaacatgg gtcaccacca aaagaagggt taggtttttt gagtcatatt gtagggttat
     6361 ttggacaacg attgtatttt atcaacaaaa caaatatgta tacagataaa ctaaaaatta
     6421 atactgagga atgtattgta tgtggtaaat gtgttaattt atgtccaatg aagaatctag
     6481 taattaaaaa ttgtatgatt gttgcaaatg ggcaatgtac aatgtgttat cgttgtgtaa
     6541 ataattgtgc taaaaaagca attacattgt taggtaaaaa agttgttgaa cagagtaata
     6601 ttaaaaaata tatgtaagaa atttaaagtt ttattgaatg taaattgatg tatattttat
     6661 gttagaaaga gaggttaaat aatggaatac tacatagttg attcatttgc aactaaacta
     6721 tttaaaggaa atcctgctgg agtttgtgtt cttgacagaa gaataccttt agagttaatg
     6781 cagaaaatag cagaagaaaa caatttacca gaaacagcat ttgtagttaa aggtaaagga
     6841 aattatgaat tacgatggtt tactcctaaa gcagaaattg atttatgtgg tcatgcaact
     6901 ttagcagcag cttatgtaat atcaaatttt attgatgtta atgtaaaaag gatagatttc
     6961 tttactcaaa gtggaaagct tgaagttaca cgaaatggaa atctatatga aatgatattt
     7021 ccagaaataa tgcctattga aatagagtta tcaccacaac aagctaattt gataggctgt
     7081 gtaccaagtg ctgtttattc ttcaagagat ttaatcttat tgctaaacag tgaacaagaa
     7141 gttataaatt ataaacctaa ttacgcacaa ttgcgtaaat tgacagactg gttaggtata
     7201 ataatcacag cacaaggaag taatacagat tttgtatcaa gatatttttg tccagaatta
     7261 gattcagaag accctgttac tggttcatca cattgtaatc ttattccata ctggtctgaa
     7321 aagttgggaa aacacaaaat ggtagctgct caactttcta atcgtggtgg tataattcag
     7381 tgtgaagtgt tgaaggataa tactgtgaaa atttctggtg aagcagtctt gtttatgcag
     7441 ggaacaataa agattgatat ttaaataagt ataataacta atttattatg aaaaataatt
     7501 aattttctta gtgttaacaa taataaagta gagttaaaaa acttgatttc acttaaatca
     7561 atatgataga tgttgatact aagattgaca agcatgatta aaaaagataa tatttaatct
     7621 ataaagataa aaacacagtc tggttggtag tccagacgca gcaatagctg tcagtaacct
     7681 gcctccttgg ttgtccgttc tttagaaagt aatttaagga ggaactagca atggacaaaa
     7741 ttagtggaaa attaacagtc ttatttgaag aaccattttg ggtaggtata tttgaaagac
     7801 aagatggtaa aaaatatgaa gcatgtaggg tagtttttgg agcagaacct aaagaagtag
     7861 aagtgtatga atttatactt gaaagatttt tctcattaga ttttggtagc attaaattag
     7921 aaaaaaatgt gaccaaagac aacattggat acaaaagaat gcagagaaaa gtaaaaaagg
     7981 aacaagaaaa agaaactata gggactaagg ctcaaaatgc tcttaagctt caatatgaag
     8041 aaagaaaaca ggatagaaaa aacctagcta aaaatagaaa agaagaagaa aaagaaagac
     8101 tatttaattt gaaacaagaa aagagaaaag ctaaacataa gggtcattaa ttttaattaa
     8161 atatcttttt aataattgct agaaaaaggt gtataaaatt tttaatttta tacaccttaa
     8221 tttattgaaa agaataaggt tttatatttc aattacttta aaatataaaa atatactaat
     8281 aaaaattgta aaaatagtat aaatggtata ccaaatgtaa atttaatgtg tttagtttta
     8341 tgtctaaaag aatacattcc aattatagaa cctatactac ctccaattac tgctatagac
     8401 ataagagtag cttcttttat tctccattcg tttctaattg ctcttttctt atctatgtac
     8461 attgaaaaga aagcaataga attaatcact ataaaatata ttagaataaa atttttcaca
     8521 gtcttgcctc cataaaaata tattatacat tataaaacaa gatatttgtt ttggaaagtt
     8581 aaatttgtaa ttagtacata taaaatattt gataagaaca aatataatat taattggaaa
     8641 atttaaaaaa attaaacaat tagcaaatag ggggtatatt tataagacac aaagtgataa
     8701 aatctattta gaaaataatg taaaataaca ggaggataaa gcaaaatgaa atttatagta
     8761 gaaaaagaag tttttgataa attagaaaat gtatgctttg gtgtagtagt tgcaaaagga
     8821 atagataata ctaaagaaat agaaagaata agcaaccttt tagatataag tatagataga
     8881 gtagaagatt acttcaaaga taaaaaagta aaggaatcag aagaaattat accatataga
     8941 gaggcattta gaagtctagg catgaaccca aataaattta tgagttcgat agaagcaatg
     9001 acaacaaggg ttgctaagaa taagaaactt cctcatataa atcctattgt agatttagga
     9061 aactctattt ctttaaaata tttacttcca atgggtgctc atgatatgga ttttagaaat
     9121 gatgatgtat atgttagatt ttctaaaaaa ggggataaat ttgtaccatt tggagaaact
     9181 gatgttgaat taatggaaga aggagaatta atttattcag ttggagacat ggtaaaaact
     9241 agaagatgga tatggagaca aggagaagaa ggaaaaatta ctaatagctc aaaaaatata
     9301 ttttttccta tagatggttt tactgatgct aatttagata aggtaatgag tgcaagagaa
     9361 gaattagcaa aattgttaaa ggaaatattt aattgtgaaa ttaaagtagg ttttgtagat
     9421 aaagataatc ctgaaatgga aatataataa gttgttgaat acttgttaag atattatatt
     9481 tacttaatga tgttaaaaag gtattaaaaa agaaaggtcg tagtaaattt gaaaaaaact
     9541 ataataaaga atgtaaatcc aatgattgaa aataagttta taggactttt tgaaatagag
     9601 tataaaaaca aattagatga agataaggtt tggatggtag catcaagaaa aagtagtgag
     9661 caattaaaaa atatttattt gaagaatgaa gaagatagtg tagatgcagt tgtaatagtt
     9721 gggcttcata aaagtagtaa aaaattaatt ttaataagac aatttagagt acctattaat
     9781 ggatatatat acgagttacc agcaggtctt gttgatgaag gagagtctat agatatttcc
     9841 gttgaaagag aacttagaga agaaacagga cttactcttt tagaaattaa aaaaaataaa
     9901 agtattgata aagtttattt atctcctgga atgagtgatg aatctgttgc ctttgtttat
     9961 tgtatatgtg atggtgatat aacagatgaa tttttagaac cagatgaaga gattgaagcg
    10021 cttttaattt cgcaagaaga tgcaaaagag attttacaaa gtaatcataa gatagacaca
    10081 aaagcatttt tgattttaca aatgtttgtg agtttaggag ctaaattatt tgaatagata
    10141 tatagaaaag aggttatata gaagagtaaa ataataaagt ttattttact tgtgtaaatt
    10201 ataatctctt tttttataca ataaagtata attttgtgta tttttaaata agtgatttga
    10261 ctcttaaatt ttcataatat gttataaacc aatttaagat actacaataa ttaaaattcc
    10321 aaaattattt aacatatttg caattaaaat gagaaaatgt aaaggctgaa tgattgaaaa
    10381 atttgatgta aaatagtagc ttagtttaat actatagttt gtagggttaa aaattagaaa
    10441 ttgtaaggga gataaattct atgaaaaaat tagttattat tttaattagt atcattagta
    10501 tggttagctt gacagcttgt aaaaaaagca atgaaagtaa atataataat ttaaacaaca
    10561 aagaatccgg acaaacaacc attgaaatgg acttagataa aaattatgat acttcagacc
    10621 catttgttaa tgcacgtttg ttttgtgtgt ctaatgatat agatgtttta gacacagaga
    10681 tttcttttaa aatggatgga gatagtggta ttgtggaaat aaaggacaat aaaacagatg
    10741 aaactttatg gagtaataca tggcatggaa gcgttgataa tgatacattc acaatttctc
    10801 tttctaatat acaaaaagag aaagagtatg ctatatgctt tactggaatt aaaataaatc
    10861 atgcagttgt aaaggttact tttgaaagca atttagttaa ggagaaagaa aaaccctcaa
    10921 aataaattcc agcttttcta taacttgata ttcagtacat aaaagcagtt gatatttatg
    10981 attgactgct ttttcttatc aaaaaggata ggttgtgcat ttttacttga aatagtttat
    11041 gcacatcaaa agttggtgag catgatattg gaaatggtaa gcatgaaatc ttgataatac
    11101 aaggaaataa atttaagttt tcaattaagt atatttccat aatttagtta acaataagct
    11161 aattattatt tatattttga tatataaata ataattgtag caaattttaa aaaatcatat
    11221 attttttgaa ctaaataaaa atttattttt attattttaa attagaggag attcttgaag
    11281 atatattatt tttaaaaata agtatagttt aaaaattcaa aaaaaagatg gcaaaatatg
    11341 tcccaaggga cattgagtaa aataaatgat ttcagcttat ttacactaaa aagtacatac
    11401 taaatgaggg gtaaaataaa gaaaaatgat atactaattg tcttttgata ataaaaaaac
    11461 atggaatttg attatttaat caagaaaaag tattgaacaa atgatttatt tagtgtacaa
    11521 taatcatgta aaaacatagt aatggaggga gacatttcat aatgaaaatt aaggtaggaa
    11581 tgaacggatt tggaagaata ggaagagctg tattaagaat agctcaagaa gaattaggag
    11641 ataatataga aattgtagcc ataaatgcaa gagcaacaac agaatctttg gcacatttat
    11701 ttacatatga ttcatgctat ggaactttta gaggtgaagt agaggcaaag gatgaagaca
    11761 ctctagtagt aaactctaaa gagataaaaa tattaagata taatgaccca gaagaattac
    11821 cttggaaaga attgggggtt gatatagtaa tagaatcaac aggattattt acacaaagag
    11881 aaaaggcaga aaaacatatt aaagcaggag caaaaaaagt aattataaca gctccaggaa
    11941 aaaatgaaga tataactata gttatcggag ttaatgagaa agaatatgat aatgaaaagc
    12001 acaatataat ttcaaatgca tcttgtacta caaattgtct agctcctttt gctaaagtat
    12061 tggacgaaaa atttggtata gtaaaagggt taatgactac agtacattca tatactaacg
    12121 atcaaagaat attagataaa agccataaag atttgagaag agcaagagca gcggctgaat
    12181 ctataatacc taccactaca ggagcagcaa aagctgtgtc aagagtacta ccacaattag
    12241 aaggaaaatt aaatggtttt tcacttagag taccaacacc aacagtttca cttgttgatt
    12301 tagtgtgcga acttaaagaa aacgttacat ctgagcaagt taactctgtg ttaaaagatg
    12361 ctgctgaagg agaattaaaa ggtgttttag gatattgtga caagccactt gtttctatag
    12421 attataaagg agattcaaga tcatctatta tagacgctct atccacaatg gttatagaag
    12481 acaatatggt taaggtagtt tcttggtatg ataatgaatg gggatattct tcaagaactg
    12541 ttgacttagt aaaatatgta gctgaaaaat taaaataata tatatttctt aaagcaaatt
    12601 ttaaaaaaat tttttaaaaa aattatataa aactaaaaaa aaagattatg aaaaatattg
    12661 aaggaaaaat tttttcataa tcttttttct ttcatgcaaa aagatacaag ttacaactta
    12721 tatttgcata ttaggtcgtt agagaagtat atatatattt agtaaggaat catacttttt
    12781 ataaaatgaa agataagatg tcgaaaactt gcataaatct gaaaaatttt tattattaaa
    12841 atactgattt ccattttaaa aatgataaaa aaatgacaaa taaagttgca aatagtaata
    12901 cttataagta taattaaaaa agagaaaagg ttttgttaaa ctttataaca aaaaaagaag
    12961 ggatgattca aaatggagaa gttaacagac aggagacagg ttaaggcagt aagcatggaa
    13021 accttcattt tcctaatatt actagtagtt ggttttgggt atgttggtag tattatggga
    13081 gcaggaatga tgttcaaagt aataatgagt acagcacatg ctttactatt ggagacagtt
    13141 ttcttaatta tggctatggc agttcttgca ggcgcattaa gcgcactact ttcagagttt
    13201 ggagtaatag cgttaattaa taaaattttc gctgtattta tgaagccttt atatggacta
    13261 cctggtgcta gtatagcagg agctataaca acttatttat cagataatcc agctattata
    13321 ccttttgcta aagataaaac attcacacag tattttaaac aataccaagt tccagcatta
    13381 tgtaacttag gaacagcctt tggtatggga cttatcttaa caacatttat gatttctcaa
    13441 ggtacagaat atgtacttcc agcattaatt ggtaatttag gtgcaattat aggtagtgtc
    13501 ataagtgtta gaataatgtt gacatttact aagaagtttt ataaatacaa tccggaagaa
    13561 gataaagcaa caggaacatt agagaaaaag gatgaattta gagaaatcag agaaggaaat
    13621 gtatttcaaa gagctttaga tgcaattctt gaaggtggta aaatgggggt agatatggga
    13681 atggctataa ttcctggagt cctagtagta tgtacattgg tgatgttatt aacttttgga
    13741 ccatctacag atcctgttac aggtcaagaa gtttatacag gagctgcata tgaaggaata
    13801 aaattacttc cagttattgg agataaactt ggatttatat tagaaccatt atttggattt
    13861 acatcaccag aggcaatagc attccctata acttcattgg gagctgttgg cgcagcaatg
    13921 tctctagttc cagagtttat aaagtcagga gctataactc ctaatgatat agcagtattt
    13981 acagctatgg gtatgtgttg gagtggatat ttaagtacac atattggtat gatggatgca
    14041 ttaaatgcta gacagttggc aggtaaagct attctttctc atacaatagg tggtttatgt
    14101 gcaggagcag ctgctcactt tatatttaca ctcgtaggat aaatgtaatc agctaagata
    14161 aaaaataata tacaaaaaat ggttgttgat atgtaataag aatttacata tcaacaacca
    14221 tttttttctg actaaagata atttataata cataaacatt aagtggatat tagagttaag
    14281 ttatatttat gtaaaaagta acaaaaatct gtagaaattt atgacaaaat ttagtacact
    14341 aactatatgt tcttatttaa aatttaaggt tagaattagg aattacctag tagagtaagc
    14401 tttgattaat aaaagaatca taaaataaaa atattagtca taaagattat aaataactta
    14461 cttaaaataa agccatagaa cttacaaaaa ttaaaaaagc atataaatat ggaggggtac
    14521 aatggatgaa ttatttcata taggaattga tgtaggttct acaacggtga aggttgtggt
    14581 acttaataat agtaataata tagttcataa agaatataga agacattatt ctgatgtaaa
    14641 aaaatcagtt aaagaagtac taaatggtat atatgaaaaa ttgggagata taaatactac
    14701 cattataata actggttcag gtggaattgg aatatcaaaa aaattaggtg taaaatttgt
    14761 tcaggaagtt atatctagta caaaatcaat agagtatttt catcctgaaa ctgatgttgt
    14821 catagaactt gggggtgaag atgcaaagat aacatatcta agtggaggaa tagaccaaag
    14881 aatgaatgga atatgtgcag gtggcactgg agcattcata gaccagatgg catcactttt
    14941 aaagacagat gccagtggat taaatgaact tgctaaaggg tataatgtta tatatcccat
    15001 agcttcaaga tgtggtgttt ttgctaaaac agatattcaa cctcttataa atgatggagc
    15061 caatcagact gatatagcaa tgagtatttt caatgcagtt gtcgtacaaa ctgtgagtgt
    15121 cctttcttgt ggaagaaaaa tagaaggaaa tgttgcgttt ttgggtggtc cattgtactt
    15181 tttatctgaa cttagagaag catttaaaag agtattagat ttaaaagatg aaaacataat
    15241 cttcccagaa aatgcacaac tttatattgc tattggagca ggattattat cagtagatga
    15301 agataatata caactaaaat ctctaataga aaaattggat agtataaaga atattgaaga
    15361 tggagaagta aatttattag aacctttatt taaagataaa aatgagtatg aagaattttc
    15421 aagaagacat gaaaaagaaa aaataaatta tgtggacata aattcaataa aaagcaattg
    15481 ttatttagga attgatgcag gctcaactac aactaaagca gcacttattg atgaggatgg
    15541 aagacttgta tattcttatt ataatagcaa tgaaggaaat ccattaaaaa caaccattaa
    15601 agttataaat gaagtatatg atatattacc aaaaaatatt aaaatattga gttcaactgt
    15661 aactggatat ggagaaggtc ttattaaaaa agctcttaaa atcgataatg gagagataga
    15721 gactatagca cattacaaag ctgctaaatt tttcaacaaa gatgtagatt ttatacttga
    15781 tattggtggt caagatatga agtgcctaaa aataaaagat ggagtaattg ataatataat
    15841 attaaatgag gcttgttcat caggttgtgg ctcattttta gagacatttg caagttcttt
    15901 atctatgaca atagaagaat ttgcgcatga aggtatttat tctcaaaatc ctgtagattt
    15961 aggttcaaga tgtactgtat ttatgaattc tagagtaaaa caagctcaaa aagaaggtgc
    16021 aagtgttgga gatatatcag cagggctatc ttattcagta ataaaaaatg ctttatttaa
    16081 agtaataaag attagagatt taaatgagat tggtaataat attgttgtac aaggtggaac
    16141 cttttataat gatttggtgc ttagaagctt tgaaaaacta attggaaaaa atgtaattag
    16201 acctaatatt tcaggaatta tgggagcttt tggttcagca cttatagcta aagaaaaata
    16261 tgaaagtgga tacaaaacat ccttgttatc taaggaagag ttgaataata tcaagctaga
    16321 agcaagtgta actagatgta aaggttgttc aaatcattgt cttcttacaa taaataaatt
    16381 ttctgataat gaaattttta tttcaggtaa tagatgtgag aaaggtgaag ctatttatgg
    16441 tgataaaaaa ataggattag aaaagaaaaa accaataaat ttatttaagt acaaatacaa
    16501 tagaattttt agatacaaac cattggaaaa agaagaagca aaaaatgggg aaatagggat
    16561 accaagagta ttaaatatgt atgaagatta tccattttgg tttacttttt tcaatgctat
    16621 gggctttaga gttgtacttt cagataggtc atcaaaacag ttatatgaaa ctggtataac
    16681 tagtattgca tctgaaacag tttgttatcc aggaaaatta gttcatggac atatagaaaa
    16741 tttaattgca aaaggtataa aaaatatatt ttatccaagt gtaacaaatg aaaataaaga
    16801 ggattcaaat gcagataatt attataattg tcctgttgta atatcatatt ctgaagtaat
    16861 aaaaaacaat gtagagaata taagaaataa gaacataaat tatataaatc catttataag
    16921 tctaaatgac aaggaaaaat tgaagaaaag actctatgat gaattatcaa gacatttttc
    16981 tgatataaaa ataactaaag aagaagttaa tcatgcagtt gataaagcta ctgatgaaca
    17041 gaatttattt aaaatggaga tacaagcagc aggtgaaaga gcacttaaag aaataaaaga
    17101 aagaaatatg aaggggattg tgttatgtgg aagaccatat catatagacc cagaaataaa
    17161 tcatggaatg ccagagctta taaattcatt agatatggca gtgcttactg aagactctat
    17221 atgtcattta gccaatgtac aaagaccact tagagttgta gaccaatggg tatatcattc
    17281 aagattgtat aaagcagcta gttttgtaag agataaaaac tatttggaac ttgttcaact
    17341 taattcattt ggctgtggat tagatgcagt aactacagac caggttcaag aaatacttaa
    17401 tgaaaaatcg aagatatata ctattataaa gattgacgaa ggcaacaatt taggagcagc
    17461 taagataagg atgaggtcct taaaagcagc tatgtttgag agagaaagca aaaatataga
    17521 tgtaaaaaat ataaaggtaa acaggataca gtatgctaaa aataatcaga taacgaacaa
    17581 acatactata ttagcaccac aaatgtctcc aatacatttc caattccttg aaaaagctgt
    17641 taacttaagt ggttataata tagaaattct aaaagatact gatagtagtg taattgaaga
    17701 agggttaaga tatgtaaata atgatgcatg ttatccagct ataatagttg taggtcagct
    17761 aataacagca ttaaaatctg gcaaatatga tttaaataac acttctgtta ctataactca
    17821 gactggtgga ggttgtagag ctacaaatta tattggattc ttaagaaagg ctatatacga
    17881 tgctggattt aaagatattc ctgtcattgc acttagtgta aatggaatag aagatagtgg
    17941 tataatggat aatatatcac taaaattaat caatagactc tttatgtctg ttgtttatgg
    18001 tgatttgctg atgaaagtac tatatagagt aagaccatat gaaaaagtta ctggaagtgc
    18061 aaatgctcta tatgaaaaat gggttgatat atgcaaatct tctcttgtta aagcaaaaat
    18121 ttctgtattt actagaaata taaaagatat agtaaaagaa tttgataatt tagagatact
    18181 agatatcaaa aaaccaaaag taggtttggt tggagaaata ttggtgaaat tccatccaat
    18241 tgcaaataat aatttagtag atatattaga gcgagaaggt gctgaagcag tcgtgccaga
    18301 ccttacaaat ttcttcttaa gttgtgcctt taatactata tacaaacaca ctcatttaga
    18361 aggaagtaga aagagtagaa tgataggaga agcatttata tacataactg gaatatatca
    18421 aagagtatat aaaaaagcac ttgataagag tgagagattt tatgctccag ccaatataaa
    18481 aaatgttgct aaaagtacag aaccagtagt gtcattaggt aatcaaactg gagaaggttg
    18541 gcttttaaca ggtgagatgg ttgaattatt aaatgaaggt gtagaaaaca ttatttgtat
    18601 gcaacctttt ggatgtttac ctaatcacat aattggtaag ggttctataa aagaattaaa
    18661 gaggttgtat aaaaatgcaa atataatacc tattgattat gacccttctg caagtgaggt
    18721 aaatcaactt aacaggataa aacttatgct ttctaaggct tttaaaaata tataagattt
    18781 atattttgat atgttaaaag ttaatgaatt taaaaatata ctctatatta agtatctata
    18841 aaaaagagtt atcctatttt ttagggtaac tctttattag ttaaaaaatt atatgtatac
    18901 tgatattaaa ggggggaagt aatatgagtt tagtatatga tgtttataaa tcaaaattag
    18961 gaaatttgta catattaagt gaagatggtt ttatagttag tatatacata ggagatgaaa
    19021 aatttaataa tttaaaaaat gaatgtatta aaaaaggaat tgatataaag agaagtaagg
    19081 aagatttaaa ggaagcaata agccagatag atgattactt taatggaaaa agaaaagtct
    19141 ttaatttaaa tattcgtatt gaagggactg aatttcaaaa atcagtttat agggaaatgt
    19201 taaaaatacc ttatggagaa acattgtgtt attcagatat agcaagaaat ataaacaatc
    19261 caaaatcagt aagagcaata ggacaagcaa gtaaaagtaa taaaattcca ataataatac
    19321 cttgtcatag agtagttggc aaaaaagata ttggtggata tatggggaat cattctgatt
    19381 tgaaagagat actattaaat ttagaaagac agagttaact tagaaagaaa tgtttttatt
    19441 gttaatattt gatataataa tagtaaaaag aagtattttt tacaaaaaca atgaagaatt
    19501 tattttaatt tataaaaatg agggggaatt ttaatgtgtg ataaagttat agcatcaaaa
    19561 tatcatgaga agggatttaa ttgtgcagaa tcagttataa aagcatataa tgaggaattt
    19621 aatacagata taccagtttg tttaggaagt ggactgggtt cagggtgtgg ggttgctagt
    19681 ttatgtggtg cagtaaatgc atcaaatata ataattggat atgtaaaagg cagaaaccac
    19741 gaagatgaaa gtactaaagc taaagtatat gctaaagatt taactacaac agtaagaaaa
    19801 gagtatggtt cagagttatg tatagaccta aaaaaagatt tagttgcttg tagagaaata
    19861 atggatttcg catatgatag tctaaaggaa actttaaaaa aagaattata agaaaataag
    19921 ctatcaagta taaattattt gatagcttat ttttatagtc gatactaaat aaatcaaata
    19981 tattttagaa ttattatttg taaataaaca gcaaataatt ttaggatttt tatttaatat
    20041 agttttt
//
LOCUS       DAELLV010000046        20011 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.046, whole genome shotgun sequence.
ACCESSION   DAELLV010000046 DAELLV010000000
VERSION     DAELLV010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20011
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.046"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(71..532)
                     /locus_tag="KPD58_001409"
     CDS             complement(71..532)
                     /locus_tag="KPD58_001409"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HBG0265187.1"
                     /translation="MIYEGKLIGKDLKIGIINSRFNEFITSKLLSGAEDCLLRHDVSP
                     ENIEIVWVPGAFEIPLVAQKMAKSGKYDAIICLGCVIRGATSHYDYVCSEVSKGIAKV
                     SLDSELPVIFGIVTTENIEQAIERAGTKAGNKGYDCAMNALEMANLFKSLN"
     gene            complement(637..1830)
                     /locus_tag="KPD58_001410"
     CDS             complement(637..1830)
                     /locus_tag="KPD58_001410"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HBG0265188.1"
                     /translation="MFNTIEEAIEDIKNGKMVIVVDDESRENEGDLLMAAEMATPESI
                     NFMATYGRGLICLPATEKKFKSLNIPLMVRENTDTFQTAFTVTIDGADTLTGISAYER
                     AETVKLFCDENSTSKDFKTPGHIFPLIAKTGGVLVRDGHTEASVDLARLAGFKEIGLI
                     CEIMKDDGTMARVDDLMIFKEKHNLKIITIKDLIEYRKINETTIEKVSSAFLPTKYGN
                     FEIIGYRDTYSNEEHIALTYGNINIENTLVRLHSECLTGDVFHSLKCDCGLQLESSMK
                     KIVENGSGVLIYMKQEGRGIGLLNKIKAYKLQEEGYDTVEANLMLGFEEDMRDFYMAA
                     QILKNLNIKSINLLSNNPDKINQLEKYGIKIENRIPINEEINDFNKLYLKTKKDKMGH
                     LLDII"
     gene            complement(1853..2518)
                     /locus_tag="KPD58_001411"
     CDS             complement(1853..2518)
                     /locus_tag="KPD58_001411"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HBG0265189.1"
                     /translation="MFTGIVEEVGILRKITANGKSGKVTILSNKILDGTNLGDSIAVN
                     GVCLTVSNLGKNEFTADVMMETIRSTNLGLLNANDKVNLERAMSLSSRFGGHIVTGHV
                     DGKGTICKFEKDENAVLVSIRPDKKLLSSMILKGSVAIDGVSLTISYLDDEIFKVSII
                     PHTKINTILLTKNVGDFVNLESDVIGKYVNNFMANNYKELNSNSNNHKSNIDKDFLFK
                     NGF"
     gene            complement(2552..3664)
                     /gene="ribD"
                     /locus_tag="KPD58_001412"
     CDS             complement(2552..3664)
                     /gene="ribD"
                     /locus_tag="KPD58_001412"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HBG0265190.1"
                     /translation="MNFVNQKEKDIYYMKKAIELAKNGEGFVNPNPLVGCVIVKASNI
                     IGKGYHEKFGSNHAEVNAINSAKQSLKDSTLYVNLEPCSHYGKTPPCVDKIIQNKIKR
                     VVISTLDPNPLVCGNGVKKLKDNNIDVTVGILEDEARDLNEAFFYHIKNKRPLCIVKS
                     AVSLDGKIATKSLESKWISNESSRYLTHKYRNKYQSIMVGINTVLNDNPLLTCRLNQE
                     KVSHPTRIVIDTHLKLPLNSNLVKDKTSKTIVFTCCKESIKLSMLKENNVETIISPSK
                     NNLVDLEFVMYKLGELNIDSVLVEGGATLNDSLFRNKLVDKVKLFLSPKIIGGKDAPT
                     FVSGEGINHLSDSTQLTINNVTLIDGDILIESDVLN"
     regulatory      complement(3796..3917)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            4106..6589
                     /gene="recQ"
                     /locus_tag="KPD58_001413"
     CDS             4106..6589
                     /gene="recQ"
                     /locus_tag="KPD58_001413"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HBG0265191.1"
                     /translation="MNKKLLEILSKYYGYTSFRKGQESIINSILSEKDVLAIMPTGGG
                     KSICYQIPALILDGMTIVISPLISLMKDQVDALKTMGISAAFINSSLSSKEFNEILNN
                     IRKNNYKILYIAPERLDAQEFIELINNNNVSQVAIDEAHCVSQWGHDFRLSYRRISDF
                     IKNLPKRPIVTAFTATASEEVRTDIINLLCLENPDYYITGFDRENLSINIVKSSSKNK
                     YILDYIQNHKNESGIIYVATRKEVENIYNGLSKRNISVSKYHAGLSKNERSKNQEDFI
                     NDNVDIMVATNAFGMGIDKPNIRWVIHYNMPQSIENYYQEIGRAGRDGEKSECVLLFS
                     PGDVHIQKYLIDIGVENPERKLFQHKKLQYMIDLVYSNSCYRKTILSYFGENYIDNCN
                     NCSNCLVEGEIVDKTIDAQKVISCVARMKRSYGVTTIVDVLRGSKNKKILQLGLNTLS
                     TYNIMRDYSSEDLKNFVNTLVSHGFLDLVETLGNGNRGSFPTIRLNEMSMKVLKGEVK
                     VEFKEIVMTKSLEVEDELYNALRELRHSIASEERIAPYMVFGDGTLRAMSSSYPINKE
                     EMLDISGVGEIKYQKYGRDFEAVIKEYVEKNNINKKIDEKNVDSKNVNSDFLEVNTDK
                     VLYEKLLVIREEYATKEKLPAYMILANKALKEISGRYPLDEEQLKDISGIGGVKFEKY
                     GASILAEVNKYVKENNINVIWEKKGKRKLILDGESRKNNEIALDLLNQGKDIQSISQE
                     IEVSLSTIIGYVHDYVKDGHSISFELGLENFYNEDKKKMILEVCEKVGYDNLNNIKRK
                     LPDSIKYENIRAVILDSYLENLKNNIIAE"
     regulatory      6678..6789
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6931..8232
                     /gene="thiC"
                     /locus_tag="KPD58_001414"
     CDS             6931..8232
                     /gene="thiC"
                     /locus_tag="KPD58_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HBG0265192.1"
                     /translation="MNYTTQMDAARKGIITKEMEIVSQKEQVDVNELRELIANGQVVI
                     PANKNHKSLSAEGVGKNLRTKINVNLGISRDCKDIEKELEKVRVAIDMKAEAIMDLSN
                     YGKTREFREKVVEMSPAMIGSVPMYDAVGYLEKELKDITEEEFLNVIRQHAIDGVDFI
                     TIHAGLTRSVCQKIKNHERLTHIVSRGGSLLFAWMELNNKENPIYTNFDKILDICEEY
                     DVTLSLGDACRPGCIKDSTDGVQIQELVVLGELTKRAWERNVQVMIEGPGHMAIDEIE
                     ANVVLEKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGALACAKGVDFLCYVTPAEHL
                     RLPNLDDMKEGIIAAKIAAHAGDIAKNVKGAREWDNKMSKARADLDWCEMFRLAIDPE
                     KAKRYRDESTPTHEDSCTMCGKMCSMRTVKKILNNEELNLI"
     gene            8286..8480
                     /gene="thiS"
                     /locus_tag="KPD58_001415"
     CDS             8286..8480
                     /gene="thiS"
                     /locus_tag="KPD58_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HBG0265193.1"
                     /translation="MKVNGKEIEFEKDLTVIDLLNKYNLKSDRVVVEVNLEIIEESNY
                     NTYVLKDEDIVELISFIGGG"
     gene            8473..9276
                     /gene="thiF"
                     /locus_tag="KPD58_001416"
     CDS             8473..9276
                     /gene="thiF"
                     /locus_tag="KPD58_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HBG0265194.1"
                     /translation="MVKIKIFVNELLTDVEENTTAYKVRDSYNNKCDVLVLNGYPIKT
                     DMTLCENDKLTLIQKGVKPSLDELESLLIARHTPNIHNKLKKGKVAILGLGGLGSNIA
                     ISLARIGVGELLLIDYDVVEPSNLNRQQYFIDDIGKLKTDAMIENIKKINPFIKLNKR
                     DIFLNKNNMDTIKDSDLIIEAFDDASCKAQVCNYVLINLKDKYLIASSGMAGYYDSNI
                     ITTKKIKDRFYICGDFVNEAKFGEGLMAPRVAICANHMANLATQILIDM"
     gene            9339..10109
                     /locus_tag="KPD58_001417"
     CDS             9339..10109
                     /locus_tag="KPD58_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HBG0265195.1"
                     /translation="MDKLVLGGHEFNSRLLVGTGKYGSNNILPEVIKESGSEIITMAL
                     RRVDLDNKQENILTYIPKEMTILPNTSGATNAEEAVRIARISRKMGCGDFIKIEVISD
                     TRYLLPDNEETIKATKILADEGFIVLPYMTPDLYAGRRLIEANAAAVMPLGAPIGSNR
                     GLQMKEMIRIMIDELDIPIIVDAGIGKPSQAMEAMEMGADAVLVNTAIASAGDPVQMA
                     RAFKLAVEGGREAYIAKTGNVSEFANASSPLTGFLGNL"
     gene            10126..11229
                     /gene="thiH"
                     /locus_tag="KPD58_001418"
     CDS             10126..11229
                     /gene="thiH"
                     /locus_tag="KPD58_001418"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HBG0265196.1"
                     /translation="MSFYDVIEKYRDFDFDGYLNNVTDNDVLRSLSKDKLEDFDILNL
                     LSKTAVKHLEDMAQKAHKLSVQYFGKTVCLYTPMYIANYCVNQCVYCSYNIKSGIKRK
                     KLTMDEIREEGEVISKEGFKHLLVLTGESSFHSSVEYIGEAIEILREKFPSIGIEVYP
                     MEVEEYKYIVDKGVEGLTVYQETYDEEIYKRVHIKGPKSNYKFRLDAPERGAKAGMRT
                     LSIGALLGLNDFRKETFFTILHGKYLKTKYPHIELSYSTPRMRPFKGCFEELVDISDT
                     DLVQAMVCMRLFDPHAAINISTRENLEMRSHIIPLGVTKLSAGVSTDVGGHSQDEHDT
                     AQFKINDESTVKDVEKMLNSIGYQHVFKDWERF"
     gene            11230..11919
                     /locus_tag="KPD58_001419"
     CDS             11230..11919
                     /locus_tag="KPD58_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBG0265197.1"
                     /translation="MYLITNRKLCSEERYLEVIKESILSGVENIIIREKDLEYQELRK
                     LYMKIKTKINCIDFQEQISDESLKTNINQKEFRNKFKVNFIINSNIEFFEKVDCQGIH
                     LPFKLFLNLIENKYNFNENKILGLSLHKVEEVDYLEKLIRNQNIKIDYITLSHIYETK
                     CKEGLNPKGIELLKEAKKITDIKIIALGGILPSNVKETLKYCDDFAIMSTIMRSKDIK
                     KTISNYNEKLN"
     gene            12107..13501
                     /gene="yfcC"
                     /locus_tag="KPD58_001420"
     CDS             12107..13501
                     /gene="yfcC"
                     /locus_tag="KPD58_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBG0265198.1"
                     /translation="MTDSKKKRFIIPHTFTIIFLLIVLMAILTWIVPSGEFSREDVEG
                     RMVVVPGTYQSVESNPQGFADVFKAPIEGFIDSAEVVGFVLIVGGAFGIVNRTGAIEA
                     GIASVISKFKGKELLIIPISMILFGLGGTTFGMAEETLPFYMIFVPLMTSLGYDSLTA
                     IAIVFIGAAAGAAASTINPFSVGIAQALAQLAPGSGVTYRVVIFIAYILISIAFVMYY
                     AKKVKENPESSIVYEIDKNNKSILNQTSTEIKEFTFKEVSVLAIFGIGMAIMIWGVLS
                     QGWYIPEISMCFAAIGVLSGLVGRLSQIEISESFIDGAKDLASAALAIALARGIVIIA
                     QNGFIIDTILNSAAAFLGGLPKVIFVYLSFFLEAALAFLIPSSSGLASLTVPVLAPLG
                     DLVNISAQVIVTAYQFGVGLTNFITPTSGVLMGALAVAHIPFSKWVRFIMPILLILTA
                     VSLVFLTIGIYIGF"
     gene            14018..15385
                     /locus_tag="KPD58_001421"
     CDS             14018..15385
                     /locus_tag="KPD58_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HBG0265199.1"
                     /translation="MGLKEFDFIEESIDMLRTMSPTLETISDEIEEYFENILDEKNQE
                     YINVTSRIKSESSLREKIIRNRYLKKYGEASNLIHNVSDLIGLRIECRFIEDENKIYR
                     LLRRYFNKTDDKINYYNKENKNIKLKLSERQPNKQKNGFEIYKIDGVFAYLDREVKFE
                     LQIKSLVNVFWSEIEHKIIYKNNTYLLADKFIKDMMDSIKNNLTMIDNQLLSIYKNFH
                     SGKSFNMKVSKKEIEKLFAKLVYDAFSEKMNKSIGFVVDFKKPCETILNYSFNKQDIS
                     DEVLGNFMLDEFARLNEIVNKDIDFNEQIEFEREPTFDDKFCKDLGNHFRSRLNTEFP
                     WNLFFRILFEIEPYNNTKDFENFVEFIKENVLLEENREQLLCQFEEDSKLIIEDIYDC
                     IFNSISEIDSVEILYNYNLEKINFTSSEIINCICREYECYGEYLEEKEKLMNTFKEKI
                     IQIFE"
     gene            15410..16018
                     /locus_tag="KPD58_001422"
     CDS             15410..16018
                     /locus_tag="KPD58_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HBG0265200.1"
                     /translation="MRHFSNIYELSKIILEEAICIEKTGVILAGGKNSRMGRDKAFLE
                     LHDKLFIEIAIEAFKNFDELIIISNNEELYSKYDIKVYNDIVKDVGPIGGIYTALEYA
                     KYDIVTIACDMPYLNNQIVERIANKMDDKSVISVTNGKLQPLCSGYKKSIINKVSLCI
                     RENDLKLRSFIDKIDKSYIYFDEEDLFLNVNTVDEYKKLTKV"
     gene            16066..16437
                     /locus_tag="KPD58_001423"
     CDS             16066..16437
                     /locus_tag="KPD58_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBG0265201.1"
                     /translation="MNLLKCDSWAVILNNSDKESKAYKILDELKRNMYKVVAIDEEKK
                     PIEGIDVYECLKDVPHNIDVVAIIDKQSKMDVILEEVELLDIQNMWFEKGSFSEMMIK
                     KTKDLKLNIEYNLSLYDELTR"
     gene            16616..16954
                     /locus_tag="KPD58_001424"
     CDS             16616..16954
                     /locus_tag="KPD58_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBG0265202.1"
                     /translation="MLIVTTEKVEGKKISKVLGLVRGSTIRAKHVGKDIGASFKNLVG
                     GELTGYNEMLTEARQIAIGRMVEDAEAKGANAVIAFRLSSASVMQGAAEMLAYGTAVV
                     LEDDNSILEK"
     gene            17420..17923
                     /locus_tag="KPD58_001425"
     CDS             17420..17923
                     /locus_tag="KPD58_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HBG0265203.1"
                     /translation="MFNVAIITLSDKGYEGKREDITGKKLTEFVENTGVYKVTEYVLI
                     KDDKEMLKENIIRLCNSNKIDLILTNGGTGFSKRDITPEATKEVLEKEIPGLSEYMRM
                     KSTEITKKAILSRGVSGIRNNSIIINLPGSPKGAVENLSFIIDVLDHGIEVLRGEATE
                     CATKKSE"
     gene            17970..18929
                     /gene="moaA"
                     /locus_tag="KPD58_001426"
     CDS             17970..18929
                     /gene="moaA"
                     /locus_tag="KPD58_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HBG0265204.1"
                     /translation="MIDKYGRKIDYLRISLTDKCNLRCVYCMPPDVKFDKNYINENLS
                     FEDYKFIIKAMAEQGITKIRFTGGEPLLYPKLNELIKFTKEECGINNIGMTTNAIGLS
                     DRIFELEKCGLNKVNISLDSLKEYKYKSITRGGNLKDVLKSIESCLNLGIKVKLNCVA
                     ISGFNDDELKDFMFLTVKSPIDVRFIELMPLGEANRVYKKGYFNVKEMIEKIYGLYKV
                     KNDEKSTAEYYMFKGAKGRIGIITPLSCSFCNTCNRIRLTSSGAIKLCLHSKEEIDIK
                     PFLHKPLLFRESMKDIIKQKPEKHHLIENKCSDTNRRMYEIGG"
     gene            18933..19403
                     /gene="moaC"
                     /locus_tag="KPD58_001427"
     CDS             18933..19403
                     /gene="moaC"
                     /locus_tag="KPD58_001427"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HBG0265205.1"
                     /translation="MLTHINEKGYAKMVDISEKDDTKRTAVATATITLSKEALEKVKN
                     GQIKKGDVLSVAQVAGIMGAKNTSSNIPMCHQIKLVGCDLEFDVDDSMSAIKIYATCK
                     SLGKTGVEMEALNACSLAALTIYDMCKAVDKKMVISNIHLVEKTGGKSGDFKFE"
     gene            19439..19870
                     /locus_tag="KPD58_001428"
     CDS             19439..19870
                     /locus_tag="KPD58_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HBG0265206.1"
                     /translation="MAKVVSINISEKKGVIKVPVQCAELKINHGIVGDAHAGNWHRQI
                     SLLGKESIDKMKMKGFDQLKYGDFAENVTTEGIEVFSLPVGTKLRIGQCEVEVTQIGK
                     KCHNGCEIKKLTGDCVMPREGIFVKVLKEGIINVNDSIDVI"
BASE COUNT         7791 a         2175 c         3423 g         6622 t
ORIGIN      
        1 tattattaaa aatagcttat ttatcgtaaa gtgaatgata ttgggtttta attattatga
       61 atgttagtat ttaatttaaa gacttaaata aatttgccat ttctagcgca ttcatagcac
      121 aatcataacc tttatttcca gcttttgtac ctgctctttc aatagcttgt tcaatatttt
      181 ctgtagttac gattccaaat ataacaggta attcactgtc taaagatact ttggctatcc
      241 ctttagagac ttcactacat acataatcat aatgagaagt agcccctctt ataacacagc
      301 ctaaacagat tatagcatca tacttaccac ttttagccat tttttgagca acaagaggaa
      361 tctcaaaagc tccaggtacc caaactattt ctatattttc tggagaaacg tcatgtctta
      421 aaagacagtc ttcagctcct gataaaagtt ttgatgttat aaattcatta aaacgagaat
      481 ttattatacc tatttttaaa tcttttccaa taagttttcc ttcatatatc atttttactt
      541 cctccaattt tatttatgtt atagatttat atttataaat atctttaatt taattataaa
      601 aaactactta gtagaaaaat atcatcatat aatcatttat ataatatcta aaagatgtcc
      661 cattttatct tttttagttt ttaaatatag cttattaaag tcattaattt cttcattaat
      721 agggattcta ttttcgattt ttattccata tttctctaat tgatttattt tatctgggtt
      781 attacttaaa agatttatac tttttatgtt taaatttttt aatatttgag cagccatata
      841 gaaatctctc atatcttctt caaatccaag cattaaattt gcttcaacag tatcgtaacc
      901 ttcttcttga agtttataag ccttaatttt atttaaaaga ccaatacctc taccttcttg
      961 cttcatataa attaaaactc cacttccatt ttccacaatt ttcttcatag aagattctag
     1021 ctgtaatcca caatcacatt ttagagagtg gaacacatct cctgttaaac attcagaatg
     1081 aagtctaact agagtatttt ctatatttat atttccataa gtaagggcaa tgtgttcttc
     1141 atttgaataa gtatctctat atcctatgat ttcaaaattt ccatattttg tcggaaggaa
     1201 agcactagat actttttcta tagtagtttc atttattttg cggtattcta taaggtcttt
     1261 tatagtgatg atttttaaat tatgcttttc tttgaaaatc attaaatcat caactcttgc
     1321 cattgtacca tcatctttca ttatttcaca gataagacca atctctttaa aacctgcaag
     1381 tctagcaagg tctactgagg cttctgtatg tccatccctg acaagaaccc caccagtttt
     1441 tgctattaaa ggaaagatgt gaccaggcgt tttgaaatct ttacttgtag aattttcatc
     1501 acagaataat tttacagttt cagctctttc atatgcagat ataccagtta gagtatctgc
     1561 tccatctata gtaacagtaa aggctgtttg aaatgtatct gtattttctc taaccataag
     1621 aggtatatta agacttttaa attttttttc agtggcaggt agacaaatta gacctctacc
     1681 atatgtagcc ataaaattta ttgattcagg agtagccatt tcagctgcca tcaataaatc
     1741 tccttcattc tctctacttt catcatcaac cacgattacc attttcccat tttttatatc
     1801 ttcaatagct tcttcaatag tattaaacat gtaattcact cctttattat ttttaaaaac
     1861 catttttaaa taaaaaatct ttatctatat ttgatttatg gttattacta ttagaattta
     1921 attccttgta gttattagcc ataaaattat taacatactt tccaattaca tcgctttcta
     1981 aattaacgaa atctccaaca ttctttgtca agagaatagt gttaatttta gtatgaggta
     2041 taatagaaac cttaaatatt tcatcatcta aatatgatat agttaaactt acaccatcaa
     2101 tagcaacaga acctttgagt atcattgagg ataaaagttt tttatcaggt cgtatactta
     2161 ctaaaactgc attttcatct ttttcaaatt tgcagatagt tcctttacca tcaacatgac
     2221 ctgttactat atgaccacca aatcttgaag aaagactcat agctctttca agatttacct
     2281 tgtcatttgc atttaataaa cctaaatttg tggaacgaat tgtctccatc ataacatcgg
     2341 ctgtaaattc attttttccg aggtttgaaa cagttagaca aactccattt acggctatac
     2401 tatctccaag atttgtgcca tctaatattt tatttgatag tatggttact tttccactct
     2461 taccattagc agtaattttt ctaagtattc caacttcttc tactatgcca gtaaacatcc
     2521 taacctccta tatgaatgta ttaacaaaaa tttaatttaa aacatctgat tctataagaa
     2581 tatcgccatc tatcaaagtt acattattga ttgttaactg tgttgaatct gacaaatgat
     2641 ttattccttc tccagaaacg aatgtaggtg catctttacc acctataatc ttgggtgata
     2701 gaaataattt gaccttatca actaacttat ttctaaagag actgtcattg agagtagcac
     2761 caccttcaac tagaacagaa tcaatgttta actctcctaa tttatacatt acaaactcca
     2821 aatctactaa gttgtttttt gaaggagaaa ttattgtttc aacattattt tcttttaaca
     2881 tagataattt tatactttct ttgcaacatg tgaaaactat agttttactg gttttatctt
     2941 taaccaaatt actattaagt ggcaacttta ggtgtgtatc tattactatt cttgtaggat
     3001 gagacacttt ttcttgattg agcctacaag ttagtaaagg attatcgttc aaaactgtat
     3061 ttatgccaac cattatactt tggtatttat ttctgtattt atgagttaga taccttgatg
     3121 attcattaga tatccatttt gattctagtg attttgtagc aattttacca tctagactta
     3181 ctgctgattt tacaatacaa agaggtcttt tgttttttat atgataaaaa aatgcctcat
     3241 ttaaatctct tgcttcatct tctaatattc ccacagtaac atcaatattg ttatctttta
     3301 atttttttac tccattacca caaacaagtg gatttgggtc taatgtagat attacaactc
     3361 tcttaatctt attttgtata attttatcta cacaaggagg cgttttccca taatgagaac
     3421 atggttctaa gtttacataa agagtagagt cttttagact ttgtttagca ctgtttatag
     3481 catttacttc agcatgatta gagccaaatt tctcatgata tcctttgcct attatattac
     3541 tagctttaac aattacacac ccaactaatg gatttggatt tacaaagcct tctccatttt
     3601 tagccaattc aatagctttt ttcatataat aaatatcttt ttctttttga ttcacaaaat
     3661 tcacaatatg tacacctcct gtttaattgg ataattttta aacaaaaaac cctgagaatt
     3721 aactcagggt ttagaataat ctacaataaa tgtttaataa atgtagaaca aaattgctct
     3781 acaaaaacac tcattaaata tcttcttcca tccagacttt actgtcggct ttggaattta
     3841 accaaatcaa ccaagtttta ccttggctcg cgggctatac cgccgattgg gaatttcacc
     3901 ctaccccgaa gattaagtat taaatttata tttagaatgt atcataaatt attttacttt
     3961 gcaaactatt tttttaaaaa atatatgtta atatataata tagaatgtct ataaactttg
     4021 aaataccacg attgtgaata taaaaataaa aatttgaaca taatcaggaa agaagtctaa
     4081 ttaatttaaa ttaggagagt aatatatgaa taaaaaactt ttagaaatac tatcaaaata
     4141 ctatggatat acaagtttta gaaaaggtca agaatctata ataaactcta tcttaagcga
     4201 aaaagatgtg ttagctatta tgccaacagg agggggaaaa tcaatatgtt atcaaattcc
     4261 agctttgatt ttagatggaa tgacaatagt aatttctcca cttatatcac ttatgaaaga
     4321 ccaagtagat gccttaaaga ctatgggaat tagtgctgct tttataaata gttcattaag
     4381 tagcaaagaa tttaatgaaa tattaaataa tattagaaaa aataattata aaatactcta
     4441 tattgctcca gagagattag atgcacaaga gtttattgag ctaataaata ataacaatgt
     4501 aagtcaagtt gcgatagatg aagcacattg tgtatctcaa tgggggcatg attttaggtt
     4561 gagttataga aggatttctg attttataaa aaatttgcct aaaagaccta tagttacagc
     4621 atttactgca acagcttcag aagaggtaag aactgatata ataaatttac tgtgtttaga
     4681 aaatccagat tattatataa ctggatttga tagagagaat ttatcaataa atatagtaaa
     4741 atcatctagt aaaaataagt atattttaga ttatattcaa aatcataaaa atgagtctgg
     4801 aattatatat gttgcaacta gaaaggaagt tgaaaatata tataatggtt tatctaagag
     4861 aaatatatct gtgtcaaaat atcatgcagg tttatctaaa aatgaaagaa gtaaaaatca
     4921 agaagatttt ataaatgata atgtggatat aatggtagca acaaatgcat ttggaatggg
     4981 tattgataaa cctaatataa gatgggttat acattacaat atgcctcaaa gtatagaaaa
     5041 ctactatcaa gaaataggta gagctggtag ggatggagaa aaaagtgagt gtgtactttt
     5101 attttctcca ggagatgtac atatacaaaa atatttaatt gatattggag ttgaaaatcc
     5161 tgaaagaaaa ttatttcagc ataagaagct tcaatacatg attgacttag tttacagtaa
     5221 ttcatgctat agaaagacaa tattgagtta ttttggagaa aattatatag acaattgcaa
     5281 taattgtagc aattgtttag tagagggtga aatagtagat aaaactatag atgcacaaaa
     5341 agtcatttca tgtgtagcta gaatgaaaag aagttatggt gttactacta ttgtagatgt
     5401 gcttagaggt tctaagaata aaaagatttt acagcttgga ttaaatactt tgtctacata
     5461 taacattatg agagattatt ctagtgaaga cttaaagaat tttgtaaata cattggtttc
     5521 tcatggtttt ttagatttag ttgagactct aggcaatgga aatagaggta gttttcctac
     5581 tataagatta aatgagatgt ctatgaaagt tttaaaaggt gaagtgaaag tagagtttaa
     5641 agaaattgtt atgacaaaat cattggaagt agaggatgag ttatataatg ctttaagaga
     5701 acttagacat tcaattgcaa gtgaagaaag gatagctcca tatatggtat ttggagatgg
     5761 tacacttaga gccatgtcta gtagttatcc aattaataaa gaagaaatgc tagatatctc
     5821 tggagtagga gaaattaaat atcaaaaata tggtagagat tttgaagcag taattaaaga
     5881 atatgtcgaa aaaaataata taaataaaaa aatagatgaa aaaaatgtcg attcaaagaa
     5941 cgttaatagt gatttcttag aggttaatac agataaagtt ttatatgaaa aactgttagt
     6001 cattagagaa gaatatgcaa caaaggaaaa attacctgca tatatgattt tagccaataa
     6061 ggctttgaaa gaaataagtg gaagatatcc acttgatgaa gaacaattaa aagatatttc
     6121 tggcataggt ggagtaaaat ttgaaaaata tggagctagt attttggcag aggttaataa
     6181 atatgtaaaa gaaaataata ttaatgttat ttgggagaaa aagggcaaaa ggaagttaat
     6241 attagatgga gaaagtagaa aaaataatga aatagcatta gatttgctaa atcaaggaaa
     6301 agatatacaa agtatcagtc aagaaataga agtttcactg tcaaccatta ttggatatgt
     6361 gcatgactat gtaaaggatg gacatagtat atcatttgaa ttaggactag aaaattttta
     6421 taatgaagat aaaaagaaga tgattttaga agtttgcgaa aaagttggct atgataattt
     6481 aaataacata aaaagaaaac ttccagattc aataaagtat gaaaatataa gagctgtgat
     6541 acttgactct tatcttgaaa acttaaaaaa taatattata gcagagtaat ttttaactta
     6601 ataaaaaata aaatttgtat tattgtgttg aactaataat tttgctgtgt tatactgaat
     6661 ttgtaaaaat aatgaaaaat tagagctagg ggcgctagaa ttggctagct gagagataaa
     6721 gccgtaactt acaaacccta ataacctgat caagataatg cttgcgaagg gaagcttttt
     6781 aatataattt tattatataa ataggcaaat tattcaatgt taggagaaat ttgtctaagt
     6841 gtatatacga ttataagagt gctttccaaa cgaggaaaag cacttttttt attttcaaaa
     6901 actataactt taatcttgga ggattgtaat atgaactata caacacaaat ggatgctgct
     6961 agaaaaggca taataactaa agaaatggaa atcgtttctc aaaaagaaca ggtagatgta
     7021 aatgaattaa gagaattaat agcaaatgga caagttgtta taccagctaa taaaaatcat
     7081 aaatctttaa gtgcagaagg tgtaggtaaa aatttaagaa caaaaataaa tgtaaatcta
     7141 ggaatttcaa gagattgtaa ggatatagaa aaagaacttg aaaaagttag agtagcaata
     7201 gacatgaaag ctgaagctat aatggattta agtaattatg gcaaaactag agaatttaga
     7261 gaaaaggttg ttgaaatgtc acctgcaatg ataggttcag tacctatgta tgatgcagta
     7321 ggatatttgg aaaaagaatt aaaagatata acagaagaag agtttttaaa tgtaataaga
     7381 caacatgcta ttgatggagt agactttatt actatacatg caggtcttac aagaagtgtt
     7441 tgccaaaaaa taaaaaatca tgagagattg actcatatag tatctcgtgg aggttcttta
     7501 ttgtttgcat ggatggaatt aaataataag gaaaatccta tttatactaa ttttgataaa
     7561 atattggata tatgtgaaga atatgatgtt acattgagtt taggagatgc ttgtagacca
     7621 ggttgtataa aagattctac tgatggtgtt caaattcaag aacttgtagt attaggtgaa
     7681 cttactaaaa gagcatggga aagaaatgta caagtaatga tagaaggtcc tggacacatg
     7741 gctattgatg aaatagaagc aaatgtagta ttagaaaaga gattatgcca tggagcacct
     7801 ttttatgttc ttggaccatt ggttacagat atagcaccag gttatgacca tataacaagt
     7861 gcaataggag gagctttagc atgtgccaaa ggagtagatt ttttatgtta tgtaactcca
     7921 gcagaacatt taagattgcc taatttagat gatatgaaag agggtataat agctgcaaag
     7981 atagctgctc atgcaggaga tatagctaaa aatgttaaag gtgctcgtga atgggataat
     8041 aagatgagta aagctagagc tgatttagac tggtgtgaga tgtttagact tgcaatagac
     8101 cctgaaaaag ctaagagata cagagatgaa tcaactccta ctcatgagga tagttgtact
     8161 atgtgtggaa aaatgtgctc aatgagaaca gttaagaaaa tattaaacaa tgaggaactt
     8221 aatctaatat aatttttggc tagtatgact ggaataaaaa taagctgaaa agaggaggtg
     8281 atttagtgaa ggttaatggg aaggaaattg aatttgaaaa agatttgact gttatagatt
     8341 tattaaataa atataattta aaaagtgata gagttgtagt tgaagtgaat ttagagataa
     8401 ttgaagaatc taactacaat acatatgtat taaaagacga ggacattgta gagcttatta
     8461 gctttattgg aggtggttaa aataaaaata tttgtaaatg aattattaac agatgttgag
     8521 gaaaatacaa ctgcttataa agttagagat agctataaca ataaatgtga tgtattggtg
     8581 ttaaatggat atccaattaa aacagatatg accctttgtg aaaatgataa attaacactt
     8641 atacaaaaag gagttaagcc atcacttgat gaattggaaa gcttgttgat tgctagacat
     8701 actccaaata tacataataa attaaaaaaa gggaaagttg ctatattggg attgggggga
     8761 cttggttcca atatagctat ctctttagct agaataggag tgggagaact tttacttata
     8821 gattatgatg ttgtagaacc atctaattta aatagacagc agtattttat agatgatata
     8881 ggaaaactaa aaactgatgc catgatagaa aatataaaaa aaataaatcc atttataaaa
     8941 ttaaataaac gagatatttt tttaaacaag aacaatatgg atactattaa ggattcagat
     9001 ttaataattg aggcctttga tgatgcttca tgtaaagcac aggtatgtaa ttatgtatta
     9061 ataaatttaa aagataaata tttaatagca tcatctggta tggcaggtta ctatgattca
     9121 aatataataa ctactaagaa gataaaagac aggttttata tatgtgggga ttttgtgaat
     9181 gaagctaaat ttggtgaagg gcttatggct ccaagggttg ccatctgcgc aaatcatatg
     9241 gcaaatttgg caacgcaaat acttatagat atgtaaatat taaaattaga tataaaaaag
     9301 attgaatatt aaaaaaattt aacgtggagg gaaataatat ggataaattg gtacttggtg
     9361 gacatgaatt taatagcaga ttattggttg gaacaggtaa atatggttca aataatatat
     9421 tgcctgaggt aataaaagaa agtggaagtg aaataataac aatggcactt agaagagtgg
     9481 atttagacaa taaacaagaa aacatattaa cgtatattcc aaaagagatg acaatacttc
     9541 caaatacatc aggagctact aatgcagaag aagctgtaag aatagctaga atatctagaa
     9601 aaatgggatg tggagatttt ataaaaatag aagtaatatc agatactaga tatttacttc
     9661 ctgataatga agaaactata aaagctacaa agatacttgc tgatgagggg tttatagttc
     9721 ttccatatat gacaccagac ctttatgctg gaagaagact tatagaagct aatgcagcgg
     9781 cagttatgcc acttggagct ccaattggtt caaatagagg tcttcaaatg aaagaaatga
     9841 taagaataat gatagatgag ttggatatac caataatagt agatgcaggt attggaaaac
     9901 catctcaagc tatggaggct atggaaatgg gagctgatgc agtacttgtc aacactgcta
     9961 tagcttcagc aggagaccct gtacaaatgg ctagagcatt taaattagct gtagaaggtg
    10021 gaagagaagc atatattgca aaaacaggaa atgtgtcaga atttgcaaat gcatcttctc
    10081 ctttaacagg ttttttaggc aatttataga aagtggtgat tggtgatgag tttttatgat
    10141 gtaatagaaa aatataggga ttttgatttt gatggatatt taaataatgt tactgataat
    10201 gatgttttaa gaagtttatc aaaagacaag cttgaagatt ttgatatatt aaatttgctt
    10261 tctaaaactg cagtaaaaca tttagaagat atggcacaaa aagcacataa attaagtgtt
    10321 caatactttg gtaaaacagt atgtttatat acaccaatgt atatagcaaa ttattgtgtt
    10381 aatcaatgtg tttattgtag ctataatata aagagtggaa taaaaagaaa aaaattaact
    10441 atggatgaaa ttagagaaga gggagaagta atttcaaaag aaggtttcaa acatctactt
    10501 gttttgactg gagaaagtag ttttcactca agtgtagaat atataggtga ggctatagaa
    10561 atattaagag agaaatttcc ctctataggt atagaagttt atccaatgga agtagaagaa
    10621 tataagtaca ttgtagataa aggtgttgaa ggattaacag tatatcaaga aacttatgac
    10681 gaggaaatat ataagagagt acatataaaa ggcccaaaat caaattataa atttagacta
    10741 gatgctcctg aaagaggggc aaaagcaggt atgaggactt tatcaatagg tgcactttta
    10801 gggctaaatg attttagaaa agaaactttt tttacaatat tgcatgggaa atacttaaaa
    10861 acgaaatatc ctcatattga gctttcatat tcaacaccaa gaatgagacc ttttaaaggg
    10921 tgttttgagg aattggtaga tataagtgat actgatttag ttcaagctat ggtatgtatg
    10981 agattatttg accctcatgc agctataaat atatccacta gagaaaattt agaaatgaga
    11041 agtcatataa tacctttagg agtaactaaa ttgagtgcag gtgtatctac tgatgtagga
    11101 ggtcattctc aagatgaaca tgatactgct caatttaaga taaatgatga aagtactgtt
    11161 aaagatgtgg agaaaatgct aaactcaatt ggttatcagc atgtgtttaa agattgggaa
    11221 agattttaaa tgtatctgat aacaaacaga aaattatgta gtgaggaacg atatttagaa
    11281 gttattaaag agtctatatt gagtggtgta gaaaatataa taataagaga aaaggattta
    11341 gaatatcaag agctaagaaa attatatatg aaaataaaaa ctaaaatcaa ttgtatagat
    11401 tttcaagagc aaatatcaga tgaaagtttg aaaactaata taaatcaaaa agagtttaga
    11461 aataaattca aggttaattt tataataaat agtaacattg aattttttga aaaagtggat
    11521 tgtcaaggta tccatcttcc ttttaaatta ttcttgaatt taattgaaaa taagtataat
    11581 tttaatgaaa ataaaatact aggattatct ttacataaag tagaggaagt agattattta
    11641 gaaaaattaa taagaaatca aaatataaaa atagattaca taacattatc acatatatat
    11701 gaaactaaat gtaaagaagg attaaatcca aaaggaattg agcttttaaa agaagctaaa
    11761 aagattactg atattaaaat aattgcactt gggggaattt taccatctaa tgtcaaggaa
    11821 acattaaaat actgtgatga ttttgctata atgtcaacta taatgaggtc taaagatatt
    11881 aaaaaaacca tatcaaatta taatgaaaag ttgaattagt aaatatagta tattgaattt
    11941 ttaacttaaa taaaaataaa aaagttatct tttaaatttt aaaaggtagc ttttttattt
    12001 tatatctttg ctaataaaaa attgtaaaaa gggaaaactt tagtcagaga gattaaacaa
    12061 tatcctagaa agatttaaaa tttagtaaag tatggaggac attataatga ctgacagtaa
    12121 aaagaaacga tttattatac cacacacttt tacaataata tttttactaa tagtcttgat
    12181 ggcaatattg acttggatag taccaagtgg tgaatttagt agagaagatg tagaaggtag
    12241 aatggtagtt gtaccaggga cttatcaaag tgtagaaagt aatcctcaag gatttgcaga
    12301 tgtttttaaa gcaccaatag aaggctttat cgattcagct gaagttgtag gttttgtttt
    12361 aatagttgga ggtgcttttg gtattgtaaa taggactgga gctatagaag caggaatagc
    12421 ttcggttatc tctaagttta agggaaaaga acttcttata attccaatat caatgatttt
    12481 atttggactt ggaggtacaa catttgggat ggcagaagag acattaccat tttatatgat
    12541 atttgttcca ctcatgacat ccttgggata tgattctcta acagcaatag caattgtatt
    12601 tataggagca gcagcaggag cagccgcttc aacaataaat ccattttcag ttggtattgc
    12661 acaggcacta gctcaacttg ctcctggttc aggagtaact tacagagtag ttatttttat
    12721 agcatatata ctaataagta tagcatttgt tatgtactat gctaagaagg taaaggaaaa
    12781 tcctgaaagt tcaatagtat atgaaataga taaaaataat aaatcaatac ttaatcaaac
    12841 ttcaacagaa ataaaagaat ttacttttaa agaagtatct gttcttgcta tatttggaat
    12901 agggatggca ataatgattt ggggtgtttt aagtcaagga tggtacattc cagaaatatc
    12961 aatgtgtttt gctgcaatag gtgtgctttc tggattggtt ggtagattat ctcaaattga
    13021 aatctcagaa agttttatag atggagcaaa agatttagca tctgcagctc ttgctatagc
    13081 tttggctcgt ggaatcgtaa taattgctca aaatggattt ataatagata ctatattaaa
    13141 ttccgcagca gcctttctag gtgggcttcc aaaggttata tttgtatatt tatcattttt
    13201 cttggaagct gcattggcat ttttaattcc atcatcttca ggtcttgctt cacttacagt
    13261 acctgtactt gctcctcttg gtgatttggt taatatatcg gctcaagtga tagtaaccgc
    13321 ttatcaattt ggtgtgggtc ttacaaactt tataactcca acttcaggag tactgatggg
    13381 agcacttgca gtagcccata ttccattttc aaaatgggta agatttataa tgcctatctt
    13441 gttgattttg acagctgtat ctctagtctt tttaacaata ggtatttata tagggtttta
    13501 attaagtgta gttttattta agatatttag taatggtata aaatgataat atgttttcaa
    13561 taaattgcca ctaatttaat tagtggtaat ttgttttttg tatttaaaaa taaatgtatt
    13621 tacttgtatt ttgaattaat atctacttag acattgtttt aattgttata tttttattta
    13681 attaagcatt gatttatcat tttttatatt tcatataaca atgcaaaaaa taatttttat
    13741 atagtaaatg acaaaagttt acaataaatg tatattttct tactcattcc ttaaaattac
    13801 tcatttagtt taataactct catttcatat gtaaagttgg taaaaaaatt aaaattaaaa
    13861 gtaaaaaact aaataaatta atgttaattt gaaatatata tgaaggtttt tggagaatac
    13921 ttacttatga taggaaaaaa tgttaaaata atagtgtaat caaatataaa ggactgaaat
    13981 aattaacagt tattaaatat taggagtgta aaaatttatg ggattaaaag aatttgattt
    14041 tattgaagag tctattgata tgttgaggac aatgtcacca acattagaaa ctatatcaga
    14101 tgaaatagaa gaatattttg aaaatatctt ggatgaaaag aatcaagaat atataaatgt
    14161 aacctctaga ataaaatcag aatcaagttt aagagaaaag ataattagaa atagatattt
    14221 aaaaaaatat ggagaagcaa gtaatcttat tcataatgtt tctgacttaa taggcctaag
    14281 aattgagtgt agatttattg aggatgaaaa caaaatatat agacttttga gaagatattt
    14341 taataaaaca gatgacaaaa taaactatta taataaagag aataagaata ttaaattaaa
    14401 actatctgag aggcaaccca ataagcaaaa aaatggattt gaaatatata aaatagatgg
    14461 agtttttgcg tatttagata gagaagtaaa atttgaactt caaattaaat ctcttgtaaa
    14521 tgtgttttgg agtgaaatag aacataaaat aatttataaa aacaatacat atttacttgc
    14581 tgataaattt ataaaggata tgatggactc tataaagaat aatcttacta tgatagataa
    14641 tcagctttta agtatttata aaaactttca ttcagggaag tcttttaata tgaaagtaag
    14701 taaaaaagaa attgaaaaat tatttgcgaa actagtctat gacgcttttt ctgaaaagat
    14761 gaataaaagt ataggttttg ttgtagattt taaaaaacca tgtgagacta tattgaacta
    14821 ttcttttaat aaacaagata taagtgatga agttttaggt aattttatgc ttgatgagtt
    14881 tgcaagatta aatgaaattg taaacaagga tatagatttt aatgagcaaa tagaatttga
    14941 aagagaacca acatttgatg ataagttttg taaagaccta ggaaatcatt ttagaagtag
    15001 attaaacaca gaatttccat ggaacctatt ttttagaata ttatttgaaa tagagccata
    15061 taacaatacc aaagactttg aaaactttgt agagtttata aaagaaaatg tgctactaga
    15121 agaaaatagg gagcaattat tatgccaatt tgaagaggat agtaagctta taattgaaga
    15181 tatatatgat tgtatattta atagtataag tgagattgat agtgttgaaa ttttatataa
    15241 ctataatctt gaaaaaataa actttacatc aagtgaaatc ataaattgta tatgtagaga
    15301 atatgagtgt tatggtgaat acttagaaga aaaagaaaag cttatgaata catttaaaga
    15361 aaagattatt caaatctttg agtaataggt caatgctgaa gtggcgctaa tgcgccattt
    15421 ttcaaatata tatgaattaa gtaaaattat tttggaggag gcgatttgta tagagaaaac
    15481 aggtgtaatt ttggcagggg gaaaaaattc tagaatgggt agagataaag cctttttaga
    15541 gttacatgat aagcttttta ttgaaattgc aatagaagca tttaaaaact ttgatgagtt
    15601 gataataata tctaacaatg aagaattata ttctaaatac gatattaaag tttataatga
    15661 tatagtaaaa gatgttggtc caattggagg aatatatact gctcttgaat atgcaaagta
    15721 tgatatagtt acaattgcat gtgatatgcc atatctaaat aatcaaatag tcgaaagaat
    15781 agctaataaa atggatgata aaagtgtgat ttcagtgaca aatggaaagt tacaaccatt
    15841 gtgttctgga tataaaaaga gtataataaa taaagtatct ttatgtataa gggaaaatga
    15901 tttaaagctc agaagcttta tagataaaat agataaaagt tatatatact ttgatgaaga
    15961 agatttattt ttaaatgtaa acactgttga tgagtataaa aaattaacta aagtttaatt
    16021 agctatggtt gtaaaataga gtaggattta tatggaggag agtttatgaa tttacttaaa
    16081 tgcgattctt gggcagtaat attaaataat agcgataaag aaagtaaagc atataaaata
    16141 ttagatgaac ttaaaaggaa tatgtacaaa gttgttgcta tagatgaaga gaaaaaacct
    16201 attgaaggaa tagatgtgta tgaatgttta aaagatgttc cacacaatat tgatgttgta
    16261 gctattattg ataaacaatc taagatggat gtgattttag aagaagtaga gcttttggat
    16321 atacaaaata tgtggtttga aaaaggaagc tttagtgaaa tgatgattaa aaagactaaa
    16381 gatttaaaat taaatataga atataattta agtttatatg atgagttgac tagataaaat
    16441 tatatttctt aataataaaa atataattag ttaataaaac atattatttt atagtataga
    16501 ttggttaata aaagtacaga ttggttaatg aaacattaaa ttacaatatt tgttaaaaaa
    16561 attgtgagtt acatgatata atgtattaaa taggatacta aggagttgat atattatgtt
    16621 aatagtaact acagaaaaag tagaaggtaa aaagatatca aaggttttag gattagtgag
    16681 aggaagtaca ataagagcaa aacatgttgg aaaagatata ggagcaagtt ttaagaatct
    16741 tgttggagga gaacttactg gatataatga aatgctcact gaagcaagac aaattgccat
    16801 aggtagaatg gttgaagatg cagaagctaa aggtgcaaac gcagtaatag catttagact
    16861 gtcttcagct tcagttatgc aaggagcagc agaaatgctt gcttatggaa cagcagttgt
    16921 tttagaagat gataatagta ttcttgaaaa ataaaatata aatagttaaa aagaggcatc
    16981 ttttaaaaat tttatggatg ccttttttta tttgaaaaaa ctaatttgag aagaaatttt
    17041 aactattatg acaataaaat tagggtgata ttatataaat aagtaaaatt atattatata
    17101 atgtatggta tagactttaa aaattttatt tgtagagaaa aatatggaca atgttataat
    17161 aattatagaa attaaaagta cttacattca ttatgtatgt gacatgtata attagtttta
    17221 tatttaatac agttttgaag taatatcttc attttgagaa cgttggaata ataaaaaatg
    17281 tattattata ggtgatataa ataaaaaata aatataatat gttatattgc tgatagaaaa
    17341 gagaataaaa gaaaataggt tttattaaaa gagattttta tttataggac ttaatcaatg
    17401 tatagagggg tgagaacata tgtttaatgt tgcaattata acacttagtg ataaaggata
    17461 tgaaggaaaa agagaagata taacaggaaa aaagttaact gaatttgttg aaaatacagg
    17521 agtttataaa gttactgaat atgtacttat aaaagatgat aaggaaatgc taaaggaaaa
    17581 tattataaga ctttgtaata gtaataaaat agatttaatt cttacaaatg gaggtactgg
    17641 tttttcaaaa agagatataa ccccagaagc aactaaggaa gtgttggaaa aagaaatacc
    17701 aggtctgagc gaatatatga gaatgaaatc cacagaaatt actaaaaagg ccatactaag
    17761 taggggtgtg agtggaataa gaaataattc tataataata aatttacctg gaagtcctaa
    17821 aggtgcagtt gaaaatctaa gttttataat tgatgtttta gaccatggca tagaagtttt
    17881 aagaggagaa gctacagaat gtgccactaa aaaaagtgaa tagtatataa tctagataga
    17941 taaaaaatag acaaagaggt agataaagta tgattgataa gtatggaaga aaaatagact
    18001 accttcgtat ttcacttaca gataagtgta atttaaggtg tgtctattgt atgccaccag
    18061 atgtaaaatt tgataaaaat tatataaatg aaaatctaag ctttgaagac tacaagttta
    18121 ttataaaggc tatggctgaa caaggtataa caaaaattag atttactgga ggagaacctc
    18181 ttttatatcc taaattaaat gagcttataa aatttacaaa agaagaatgt ggaataaata
    18241 acataggaat gaccactaat gctattggtc tttctgatag aatttttgag cttgaaaaat
    18301 gtggcttaaa taaagttaat ataagtttag attctttgaa ggaatataag tataaatcca
    18361 taacaagagg aggaaactta aaagatgtct taaaatcgat tgaatcttgt ttaaatttag
    18421 gtataaaagt aaagttaaat tgcgttgcta taagtgggtt taatgatgat gagcttaaag
    18481 attttatgtt tttaacagtt aaatcaccaa ttgatgtaag atttatagag cttatgccat
    18541 taggtgaggc taatagagtt tataagaagg gttattttaa tgtaaaagaa atgattgaaa
    18601 aaatatatgg tttatataaa gtcaaaaatg atgaaaaaag tacagcagaa tattatatgt
    18661 ttaaaggagc taaaggaaga ataggaatta taactccatt aagctgttca ttttgtaaca
    18721 cttgtaatcg tattagactt acttctagtg gtgcaataaa attatgtcta cattcaaaag
    18781 aagaaataga tataaaacca tttttacata aaccattgtt atttagagaa tctatgaaag
    18841 atataataaa acaaaaacca gaaaaacatc atttaataga aaataaatgt agtgatacaa
    18901 ataggcgtat gtatgaaatt ggaggataag taatgttaac acatataaat gaaaaagggt
    18961 atgccaagat ggtggacata agtgaaaaag atgatactaa aagaacggcc gtagctacag
    19021 ctacaattac tcttagtaag gaagcattag aaaaagtaaa aaatggtcag ataaaaaaag
    19081 gagacgtatt gtctgtagct caagttgcag gtataatggg agctaaaaat acatcttcaa
    19141 acataccaat gtgccatcag attaaattag ttggatgtga tttagaattt gatgtagatg
    19201 attctatgtc tgctattaag atatacgcaa cctgtaaatc tcttgggaaa actggagtag
    19261 aaatggaagc tttaaatgca tgttctttag cagcacttac aatttatgat atgtgtaaag
    19321 cagttgataa aaaaatggtt ataagtaata tccatcttgt ggagaagact ggtggaaaat
    19381 caggagattt taagttcgag taaattaata atatattggg ttaaaatttg gaggaaatat
    19441 ggcgaaggta gtgtcaataa atataagtga gaaaaagggt gtaattaaag ttcctgtaca
    19501 atgtgcagag ctaaaaataa atcatgggat agttggagat gcacatgcag gaaactggca
    19561 tcgtcagata agcttacttg gaaaagaaag catagataaa atgaaaatga aaggctttga
    19621 ccaattaaaa tatggagatt ttgctgaaaa tgtaactaca gaagggatag aagttttttc
    19681 cctgcctgtt ggtacaaagt taagaatagg gcaatgtgaa gttgaagtaa ctcaaatagg
    19741 gaaaaaatgt cacaatggat gtgaaattaa gaaattgaca ggtgactgtg tgatgccacg
    19801 agaaggaatt tttgtaaagg ttctaaaaga aggaattata aatgtgaatg attcaataga
    19861 tgtaatctaa tgaagtcact attaaaatat aattataagt aaatactatt aagatttaaa
    19921 tatatatttt aagagagaat taattttaat aaaatatttt agttataaat tggaatattg
    19981 gttatataaa gctatgaatg taaaatcata g
//
LOCUS       DAELLV010000047        19657 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.047, whole genome shotgun sequence.
ACCESSION   DAELLV010000047 DAELLV010000000
VERSION     DAELLV010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19657
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.047"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            70..720
                     /locus_tag="KPD58_001429"
     CDS             70..720
                     /locus_tag="KPD58_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0265207.1"
                     /translation="MTNKELQKKRILMYFIEAAQNIMENEGIENITLRKVADMAGYNS
                     STLYKYFKNLDHLISFASIKYFKDYNLNISRCIENVNDEYKKYIIMWKLFCKHSFDNA
                     QAFYQVFFNLSSDELSYITKQYYDMFPEDLGIHDSDISLMITGESIKERNKILLNNLV
                     DTGYIPAKDLDIINDIIISFYQNLLFLKKNTGKNSDDDELTMKMISSIEYIIKHAK"
     gene            complement(877..1896)
                     /locus_tag="KPD58_001430"
     CDS             complement(877..1896)
                     /locus_tag="KPD58_001430"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBG0265208.1"
                     /translation="MEKVCVLGTGSWGSALALGLAKKGNDVSMWTRKEEQAKKINRTK
                     ENTDYLPGVLFPNNITISTDIEKTIKDCKVIVLAVPSQAVRSTCQKIKPFIKEGQVIV
                     NVAKGLEKGTGLRLSQVCEEELPQNPYVILSGPSHAEEVARDIPTTVVVASKDLKIAQ
                     MIQDLFMSPKLRVYTNPDIVGVELGGALKNIIAFGAGICDGLGYGDNAKAALMTRGIS
                     EMSRLGIAMGANMSTFAGLSGIGDLIVTCTSMHSRNRRAGILIGKGMSLEDTLKEVKM
                     VVEGITATEVAHDVAEKLDIDMPITNAIYSVIKKGSNPKEVGIELMMRSKKHEMEEVV
                     LGDDI"
     gene            complement(1927..2541)
                     /gene="plsY"
                     /locus_tag="KPD58_001431"
     CDS             complement(1927..2541)
                     /gene="plsY"
                     /locus_tag="KPD58_001431"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBG0265209.1"
                     /translation="MEIFSYIIIAVVAYLLGNISTSYIVAKRIAGVDIRTQGSGNAGS
                     TNVLRTLGKRAGAMTFLGDVLKGVMAVLISEFAARLVGIDTLLAGYLAVICVVAGHNW
                     PAVLGFRGGKGVATSLGAMLAVNPVITLMCLAVFILVVAITKYVSLGSVVGIGCSPIF
                     MIMVKNKAGLIVALFLTASVIYNHRANIKRLLNGTERKIGQKKE"
     gene            complement(2576..3895)
                     /gene="der"
                     /locus_tag="KPD58_001432"
     CDS             complement(2576..3895)
                     /gene="der"
                     /locus_tag="KPD58_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBG0265210.1"
                     /translation="MSRPVVAVVGRPNVGKSTIFNKFAGKRISIVENTPGVTRDRIFA
                     EVEWLDKYFTLVDTGGIEPDSEDIILSQMRNQAMLAMDMSHVILFIVDGKAGITAADK
                     EIAQLLRKTKKPVILVVNKIDSQSQFDNIYDFYELGFGTPFAVSGANSMGFGDLLDEI
                     VENFPAGLDTEYEEDIIRVAITGKPNAGKSSILNKILGEERVIVSPIAGTTRDAIDTY
                     FEKNGQKFLLIDTAGLRRKSKIYETIEKYSVIRAMSAVDRADVVLIVIDALEGVTEQD
                     TKVAGIAHDEGKGCIFVINKWDLIEKDNKTMSNYTKDIKEKFPFMMYAPIVFVSAKTN
                     QRMNKILDTVEYVSNEHSKRISTSALNEVIGEAVMLNQPPSDKGRRLKIYYGTQTDIR
                     PPKITLFINDKDLTHFSYQRYLENKIRENFGFEGTSIKFEYRQKNKK"
     gene            complement(3929..5257)
                     /locus_tag="KPD58_001433"
     CDS             complement(3929..5257)
                     /locus_tag="KPD58_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HBG0265211.1"
                     /translation="MEVNVKKVNNIISKVYKDSIAEEMGIEVGDLLISVNEQPIHDII
                     EYRFLLSDEYLDVEIQKKDGEVYIYEIEKDYDEDLGVEFTNPIIDQAKSCRNKCMFCF
                     IDQLPEGMRETLYFKDDDSRLSFLQGNFVTLTNMSEEDINNIIKYRISPINISVHTTN
                     PELRQKMISNKFAGKLYGIMKRLADAHIEMNCQIVLCPGINDGKELDRTIKELAQLYP
                     YVNSVAIVPVGITKHRENLVELNIFNDKSASKTIEQIHQIQQKYLEKLGTRFAFLSDE
                     FYILSNSELPGYEEYEGFLQFEDGVGMIRKLKTEIEEYLNILPEKILKREKKVSIATG
                     HSAYEFIQSMADAMMDKFKNLQVNVYEIKNKFFGETITVSGLLTAKDLKEQLEDKELG
                     EALYITRSMLKADEEIFLDNIELNQLEELMRIKIIPCLNEGKDFVDKILK"
     gene            complement(5495..5695)
                     /locus_tag="KPD58_001434"
     CDS             complement(5495..5695)
                     /locus_tag="KPD58_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265212.1"
                     /translation="MGMDIGLNEYTLAIVTLDKNMKNGGGCPIFYANDEKDLQNKALL
                     MSKCVGGMVHDMTNGTLIIVKH"
     gene            complement(5697..6629)
                     /locus_tag="KPD58_001435"
     CDS             complement(5697..6629)
                     /locus_tag="KPD58_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HBG0265213.1"
                     /translation="MDRVFFVAVIIGITSRIIMLNLDQKQYPTQPNVLLSQLILAFVA
                     SSLGALLVPALINRSYTSITFLSLAAEQFRQVRANRRNTLQNLEEVQLVKRGAAFIEE
                     IARTYEVRNYMCIVTSFLTIGLYYVILAETKISEGMSLVISSICGLVLAFILKKLLTR
                     KSIGDIADVMPAEISFVDESILKVGDLKGITNIGLKSDREVYINRGVGIQIIPKNKDY
                     ANAGILFDSGQRQAIVYNIYSRLGLYGDYGEPSFVPLPRRNKEDESIMIAYLPVDKNV
                     EKVMEAVKSCPILSSSKGKNLSLKNYKIGKKGSA"
     gene            complement(6674..7606)
                     /locus_tag="KPD58_001436"
     CDS             complement(6674..7606)
                     /locus_tag="KPD58_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HBG0265214.1"
                     /translation="MEESLLNANLFRHSFIVALVVGILCRGLVLRVTDKQYPSRPQDY
                     LEQIIISGLSASLGAIALPALIDKEFAALTFFAVAIQQFQGLAEQERITLKNIDNEEL
                     VPKGDAYVEEIASTYESRSYISLFSALVSSIVYIVFARKYGLSFFYCTILAIVSGAIV
                     GLIFRRFLRRNSIADIADIVPAKISFEGPILMVNGVIITNIGLESTREKYRNECLAIE
                     VIPRDLGAFGIVNDIGQRQAIIHNLFIHMGIDRDVDEVDIVAISKTNLEKSTVVIPYM
                     PILKDIDVMIDVVKSTPIIETSKGKQSDFSRKTL"
     gene            complement(7661..8950)
                     /locus_tag="KPD58_001437"
     CDS             complement(7661..8950)
                     /locus_tag="KPD58_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0265215.1"
                     /translation="MEDLNSIYFFIVMLSIAIVILAVRYTITLRKYLKEFIYVSKKVS
                     NNEYHARLNISAKGELGELARNFNHMIKNMDKTLEEVQYKHLQLRSILKSISHGILAI
                     DVNGNILLINDEAKEMIKSEPNQLVEGHNINVVIKEEEILKQILQFIGSKDNETANIT
                     TKDDIVYKIKVDPVYLQNTNNVIIGSIVNIEDITKKVKLENMRSDFVANVSHELKTPL
                     TSISGFVETLKSNENIDVNTRNRFLEIIESESDRLKRLIDDILLLSFVENKDTLLMEN
                     VVVYDVFKEVYEITQHSASEKNIEVLYKCDDKSISILSNKDYIKQVFLNLVDNAIKYT
                     QDNGIVQVVVLKDEEKIVIKVIDNGVGIPKEDVERIFERFYRVDKARSRDVGGTGLGL
                     AITKHIVKSLNGCIKVKSELGIGSEFIVTILQKNFLK"
     gene            complement(8979..9671)
                     /locus_tag="KPD58_001438"
     CDS             complement(8979..9671)
                     /locus_tag="KPD58_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0265216.1"
                     /translation="MNTKVLVIDDEMHIVELLKFNLEVSNYEVSYSYDGFDGFIKAKE
                     IKPDLILLDWMLPNISGIEVLRKIRSDKDLKNIPVIMLTAKNMENDKVEGLEIGADDY
                     ITKPFSIKELLARISSVLRRYNLTSLGEENNILTTGNLKLDLSKHEVTKGSEKIELTL
                     KEFELLKLLIQNKGKVLSRNYLLDKIWGYEYYGETRTVDVHIRYLRKKIEDEDKSEKY
                     IETIRGVGYKID"
     gene            complement(9686..10447)
                     /gene="pgeF"
                     /locus_tag="KPD58_001439"
     CDS             complement(9686..10447)
                     /gene="pgeF"
                     /locus_tag="KPD58_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HBG0265217.1"
                     /translation="MMKDYINIKNVQDIKEYVNLSGNCEELDEKLNSVNLVITCKNID
                     AKNREDMLKVCKECDLTFENLTSNSQIHSDIVTIVNKDTIGKRRDGDALITNLEKVPL
                     LLFTADCVPISIIDAKNKAIGLAHAGWRGTFSNIGHKTIKLMSECYKTVPEDLVCIIG
                     PSIGPCCYEVSEDLVEKFNTILTNNDEKFYIIKEGSYYLDLWKINEYMLRCSGVKKEN
                     IINLNLCTSCRADKFHSYRKHNKASERIGTVLQIK"
     gene            complement(10444..10905)
                     /gene="nrdR"
                     /locus_tag="KPD58_001440"
     CDS             complement(10444..10905)
                     /gene="nrdR"
                     /locus_tag="KPD58_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HBG0265218.1"
                     /translation="MQCPYCNYKESKVIDSRHTDLKSIRRRRECESCKKRFTTYEKIE
                     TTPLMVIKKDNSREYFDREKIKYGLLKACEKRPVSIDEIESIVVHIENEINKCFIEEI
                     ETKKIGEMVMDKLKELDEVAYVRFASVYRQFKDINTFVNELKSILIEKGDK"
     gene            complement(10927..11190)
                     /locus_tag="KPD58_001441"
     CDS             complement(10927..11190)
                     /locus_tag="KPD58_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HBG0265219.1"
                     /translation="MIRVSDIMEKEIINVKNGKRMGFIIDIDMDIHEGKVVSFTIFGD
                     GSRSFFSRGGDEEVIFWNDILKIGCDTIIVNIGSELSLDEFEI"
     gene            complement(11294..12067)
                     /gene="sigG"
                     /locus_tag="KPD58_001442"
     CDS             complement(11294..12067)
                     /gene="sigG"
                     /locus_tag="KPD58_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HBG0265220.1"
                     /translation="MQVNKVEICGVNTSELPVLKNKQMKELLLQIKNGDEEARQQFVR
                     GNLRLVLSVIKKFNNRGENIDDLFQIGCIGLIKAIDNFDLSQNVRFSTYAVPMIIGEI
                     RRYLRDNNPIRVSRSLKDIAYKALQVRERLIRTNSKEPTVSEIAKELELEVESVVMAL
                     DAIQDPISLFDPVYQDNGDAIFVMDQVQDKKDTDENWLQEISLKEAIKKLNSREKLVL
                     DLRFYKGRTQIEVADEIGISQAQVSRIEKNALKNMRKYV"
     gene            complement(12159..12881)
                     /gene="sigE"
                     /locus_tag="KPD58_001443"
     CDS             complement(12159..12881)
                     /gene="sigE"
                     /locus_tag="KPD58_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HBG0265221.1"
                     /translation="MLRLKERIISFITMLGIKLKIIKPKGIYYMGGANILPPPLKPEE
                     EMELLQKLETDESVKSILIERNLRLVVYISRKFENTGIDVEDLISIGTIGLIKAVNTF
                     KLNKNIKLATYASRCIENEILMYLRKNNKKKTEVSFDEPLNIDLDGNELLLSDVLGTE
                     NDEIYKIIEEEIDRDLLVMALDRLSDREKQIMELRFGLIDKGIEKTQKEVAGILGISQ
                     SYISRLEKKIISRLQKEMKKFV"
     gene            complement(12906..13778)
                     /locus_tag="KPD58_001444"
     CDS             complement(12906..13778)
                     /locus_tag="KPD58_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HBG0265222.1"
                     /translation="MFKGFKGGYMVYIEYYVIENLVINYIIISCTSILTKRYNPKKNK
                     LLGASLGGAYSVAYLYPSMNILFTVPFKIIIMSIITLISFRSKSKKDYVHVALVFYLV
                     NIFISGSTYFIIYFTGISHLKISFLIACTYVSCELLKYIYKDLKMLKYIKELTKTINI
                     NLLEKSFCCKALVDSGNLLKDPISQSDVIIVKSSVLEGFIPEKLLNINYEDIDMKKAQ
                     EIIGSLDDKTSGRVRIIPYKHAGSNKTSIIIGLKADYIEVDEQKIGNIILGISNFNDR
                     EYGAIMNPNILPGF"
     gene            complement(14068..15342)
                     /locus_tag="KPD58_001445"
     CDS             complement(14068..15342)
                     /locus_tag="KPD58_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBG0265223.1"
                     /translation="MIKLKKVLSLVMVGILSVSILAGCSKKEDSKGKEGKEKLVVWSA
                     PLTDHDADAWKPIFEKFEKENNCEIEFQIVPWDNYAEKYATAISAGEGPDIGYMYAEM
                     FPQFIEMGAVEDLTPYLEKSGTSDNYLYLDDAKMMGGIYGLPIEAANPGVLYYNKDIL
                     EKLGEKPPKTWDDFKRICEKATKDTDGDGKIDQWGLAQGWGSKVFGSLNWNWYPYLWQ
                     AGGDIYNDDLKSVKFNNAEGLKAANFINDMKKYIPEDALSKDSNEMIESVFGPGKAAF
                     TIMLSSAATSTFDKSFPDLNWGYVTSLEDKTVGSFASVDDLTLMSACKNKELGFKLLE
                     YMLSKESMEAFHKEIPRAPVAKGEKYQGDPRFEEMVTRDKHMYRPLTVGPHGVEIYEY
                     LWKQLQMMIAGDITPEQALNESAKYANGLLAQ"
     gene            complement(15626..16783)
                     /gene="ftsZ"
                     /locus_tag="KPD58_001446"
     CDS             complement(15626..16783)
                     /gene="ftsZ"
                     /locus_tag="KPD58_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBG0265224.1"
                     /translation="MLNFDVELEECAQIKVIGVGGGGNNAVNRMVEAQLKGVEFISVN
                     TDKQALYTSKAEYKVQIGEKLTRGLGAGANPEVGKRAAEESKDEIVKLLQGADMVFVT
                     AGMGGGTGTGAAPVVAGLAKEMGILTVGVVTKPFAFEGKIRMKNAEGGIAELKSKVDT
                     LITIPNDRLLQIVQKNTSMLDAFAVADDVLKQGIQSISDLIAVEGLINLDFADVTTIM
                     KDKGLAHMGIGSASGETRAIDAARQAIQSPLLETSIQGAKGVLLNVTGGPNLGLFEVN
                     EASTLVMESCDPEANVIFGASIKEDLGDEIMITVIATGFEGLQNGALDLDTKPKSSIR
                     SSLNTTVKQAVKEIEEEVIAEEKIEPPKKASIIEEDDDESMEIPTFLRRRR"
     gene            complement(16973..17326)
                     /locus_tag="KPD58_001447"
     CDS             complement(16973..17326)
                     /locus_tag="KPD58_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HBG0265225.1"
                     /translation="MLWVLGGLAFGVVVGYYIPFTYSMDYSIYMSVAILAIMDSIFGA
                     IKSNFEDNYDNVIFITGFIGNAILAILLTYIGEKLGVSLYYVAVLVFGMRLFNNLSII
                     RRYIISNIMNKKRKN"
     gene            complement(17425..18099)
                     /locus_tag="KPD58_001448"
     CDS             complement(17425..18099)
                     /locus_tag="KPD58_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBG0265226.1"
                     /translation="MKKKIVILGAFILGVFISTYIKSLDPNRVYITLGQTESIESEVE
                     NTKKEIKNLKETLKKNKKTLNEYKEAKKNGNESIQTLMQKELEEVKELSGYSTVTGPG
                     ITITMRDSERELKDGQNPNDLIIHDIDILRVLNDLKKAGARAISINGERVLATSKIKC
                     SGATITVNDTTYGQPFIIKAIGDIDLLSAAINSPESYAKSLKDIYGINIKVQENNGNK
                     VNLFAK"
     gene            complement(18104..18802)
                     /locus_tag="KPD58_001449"
     CDS             complement(18104..18802)
                     /locus_tag="KPD58_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBG0265227.1"
                     /translation="MKKRFINKSIIIGSIVLGVLVSLQLKIINIENRGMTTFKRGEQL
                     TEELKSLKKEKENLQSEVNSVKKDIETYRGKNNEGEQDILKSEIEKYETLAGYTDVEG
                     TGIKIEINSTKDLGNSDKNDSILYNYDLLLSMINKLNSAQASAISINDQRIVSNTYMY
                     LKEDKLYMNDTVIKEPIIIKAIGDSDTLASALNIKYGIVWEIETYYNAKVKVTKEDNI
                     EINGHSDINKAEGK"
     gene            complement(18825..19565)
                     /locus_tag="KPD58_001450"
     CDS             complement(18825..19565)
                     /locus_tag="KPD58_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HBG0265228.1"
                     /translation="MKKRRKLNTNNVMIVLVFILMLSFCICIIVKSDLFDVKKIDVIG
                     NKRVTKSNIMKELNVNLNENIFAYNFKDMKNKLIKNPYIENVEIKRKLPNKIIISLKE
                     KEIFAVLKDEDNYCYIDKKGNLLEELRGSNESKKDLIVDVDYSIDDNKSIKFKNYKTK
                     ENVFKTLNYLKEEGIYRKINYVNLKKESNIEMLTRSNIKILLSNDDNLDYNISRVSKI
                     LIDLQNKNTNGGTINLNYGKLAVYSPEG"
BASE COUNT         6028 a         3625 c         1994 g         8010 t
ORIGIN      
        1 gatttatggt aagtttaatg ataaaatcac gattatataa actatattga ttatttgtag
       61 gtgaataaaa tgacaaataa agaattacaa aagaaaagga tattgatgta tttcatagaa
      121 gctgctcaaa atattatgga aaacgaaggt atagaaaata ttacacttcg taaggttgca
      181 gatatggctg gctataatag ttcaactcta tataaatact ttaaaaattt agaccattta
      241 atatcatttg catctataaa atattttaaa gattataact tgaatatttc aagatgtata
      301 gaaaatgtca atgatgaata taagaaatat ataattatgt ggaaattatt ttgtaagcat
      361 tcttttgata atgctcaagc tttttaccag gtatttttta acttgtctag tgatgaactt
      421 agctatataa caaaacaata ttatgatatg tttccagaag atttaggtat tcatgatagt
      481 gacatatctt taatgattac aggagaaagt ataaaggaaa gaaataagat actattaaac
      541 aatcttgtag atactggata tataccagct aaagatttag acataatcaa tgatataatt
      601 atttcttttt atcaaaatct attattctta aaaaagaaca caggaaaaaa ttctgatgat
      661 gatgaattaa ctatgaaaat gatttcttct attgaataca taataaaaca tgctaaataa
      721 aattaaaata tggctgtctc aaaatagaga taagcctttt ttattgttga aataataaaa
      781 atatatttaa tttcatataa aaaagagtgc ctcgtaaact aaaaagttca ttttgagaca
      841 cccctaaaat aaatttatta tttattatac aatagtttat atatcatcac ctaaaactac
      901 ttcttccatt tcatgctttt tacttctcat cattagttct attccaactt ctttaggatt
      961 tgaacctttc tttattacag aatatatagc attagttata ggcatatcta tatctaattt
     1021 ttcagcaaca tcatgtgcta cttcagtagc agttatgcct tctacaacca tctttacttc
     1081 ttttaaagta tcttctaaac tcatcccttt acctatgagt ataccagctc ttctatttct
     1141 actatgcata cttgtacaag taactattaa atctccaata cctgaaagac ctgcaaaggt
     1201 agacatatta gctcccatag caattccaag tctgctcatt tcactaatac ctcttgtcat
     1261 aagggcagct tttgcattat caccataccc taatccgtca catatcccag ctccaaatgc
     1321 tataatattt tttaatgctc ctccaagttc tactccaact atgtctggat tcgtataaac
     1381 tctaagttta gggctcataa ataaatcttg tatcatctgt gctattttta aatctttaga
     1441 agcaactact acggtagtag gaatatccct tgctacttct tcagcatgag atggcccaga
     1501 tagtattaca tatggatttt gtggtaattc ttcttcacag acttgagata atctaagccc
     1561 agtccctttt tcaagacctt tagctacatt aacaataact tgtccttctt ttataaatgg
     1621 ttttattttt tggcaagtac ttcttacagc ctgtgatgga actgctaaaa ctattacctt
     1681 acaatctttt atagtttttt ctatatcagt acttatagtt atattatttg gaaataatac
     1741 tcctggtaaa tagtctgtat tttctttagt tctgtttatt tttttagctt gttcctcttt
     1801 tcgagtccac atgctcacat cattaccttt ttttgcaagc cctaaagcta atgcacttcc
     1861 ccagcttcca gtacctaata cacatacttt ctccatgttt aacaccacct gagtataata
     1921 tatttactat tctttctttt gaccaatttt tctctctgtc ccatttaata atcgctttat
     1981 atttgctctg tgattatata ttactgatgc agttaagaac agagctacta ttaaaccagc
     2041 tttatttttt accataatca taaatattgg tgaacatcct attcctacaa cagaacccaa
     2101 tgaaacatac tttgttatag ctaccaccaa tataaatact gctagacaca ttagtgttat
     2161 tactggattt acagctagca ttgcccctag tgaagttgca acacctttgc ctcctctaaa
     2221 tcctaaaacc gctggccaat tatgacctgc tacaacacat ataactgcca gatatccagc
     2281 taaaagagta tctattccaa ccaatcttgc tgcaaattct gatattaaaa cagccattac
     2341 acctttaaga acatcaccta aaaatgtcat tgctcctgct ctttttccaa gtgtcctaag
     2401 tacatttgtc gaacctgcat taccagaacc ttgagtcctt atatcaaccc cagcaattcg
     2461 ctttgcaact atgtatgagg tagatatatt tcctaaaaga tatgcaacta cagcaataat
     2521 tatataacta aatatctcca ctgaatttcc cccctttagt tatattttaa gatatttatt
     2581 ttttattttt ttgtctatac tcaaacttta tggaagttcc ttcaaatcca aaattttctc
     2641 ttatcttatt ctcaaggtat ctttgatatg agaagtgagt taaatcctta tcatttataa
     2701 acaatgtaat tttaggaggt ctaatgtcag tttgagtccc atagtaaatt tttaatcttc
     2761 tacctttgtc agatggtggt tgatttaaca ttacagcttc tccaattact tcattcagtg
     2821 ctgaagttga tattctctta gaatgttcat ttgacacata ttcaactgta tctagtatct
     2881 tgttcattct ttgattagtt tttgcagaaa caaatacgat tggagcatac atcataaatg
     2941 gaaatttttc ttttatatct tttgtgtaat tgctcatagt cttattatct ttctctatta
     3001 aatcccattt attgattaca aatatgcatc cttttccttc atcatgtgct atacctgcaa
     3061 cctttgtatc ttgttctgtt acaccttcta aggcatctat tactatcaat actacatctg
     3121 ctctatctac agcactcata gcccttatta cactgtattt ttctattgtc tcatatattt
     3181 tactttttct tctaagccct gctgtatcta ttagaagaaa tttctgtcca tttttttcaa
     3241 aataagtatc aattgcgtct ctagtagttc ctgcaattgg acttacaatt actctttctt
     3301 ctccaagtat cttattgagt atagaacttt ttcctgcatt tggtttacct gttatagcta
     3361 ctcttattat atcttcttca tactctgtat ctaatcctgc tggaaaattt tcaactatct
     3421 catctaataa atctccaaat cccatactat tggctcctga aactgcaaaa ggcgttccaa
     3481 atcccaactc ataaaaatcg tatatattat caaattgact ttgactatca atcttattaa
     3541 ccactaagat tactggtttt tttgtctttc taagcaattg tgcaatttct ttatcagcag
     3601 ctgttattcc agctttacca tcaactataa aaagtattac atgtgacatg tccattgcaa
     3661 gcatggcttg atttctcatt tgagataata ttatatcttc gctatcaggt tcaattcctc
     3721 ctgtatccac taatgtaaaa tatttatcta accattctac ttcagcaaat attctatctc
     3781 ttgtaactcc aggtgtattt tcaactattg atatcctttt tcctgcaaat ttattaaata
     3841 ttgtagactt accaacgttt ggtctcccaa ctacagctac aactggccta cttatactca
     3901 ttttatttct ccttccatct tttgttttct attttaaaat tttatccaca aaatcttttc
     3961 cttcattcaa acaagggata atttttattc tcataagttc ttctaattga tttaattcaa
     4021 tattatctaa gaaaatttct tcatctgctt ttagcatact tcttgttata taaagagctt
     4081 ctcctaattc tttatcttca agttgttctt ttaaatcctt agctgttaaa agacctgata
     4141 cagttattgt ctctccaaaa aacttgtttt tgatttcata tacatttact tgaagatttt
     4201 taaacttgtc catcatagca tctgccatac tctgtataaa ttcatatgca gaatgaccag
     4261 tagctattga tactttcttc tctcttttta gaatcttctc aggcaatata tttagatact
     4321 cttcaatctc tgtttttaac ttacgtatca tacctacacc atcttcaaac tgtaaaaaac
     4381 cttcatattc ttcatatcca ggcaattcac tattagatag tatataaaat tcatctgata
     4441 aaaatgcaaa tcgagtacct aatttttcaa gatatttctg ttgtatttga tgtatttgct
     4501 ctatagtttt acttgcgctc ttatcattaa atatatttaa ttctactaga ttttctctat
     4561 gctttgtaat cccaactgga actatcgcaa cactgtttac atacggataa agttgagcta
     4621 gttcttttat tgttctatcc aattcctttc catcatttat acctggacat aaaacaattt
     4681 gacagttcat ttctatatga gcatctgcca atctcttcat tataccatat aattttcctg
     4741 caaatttatt gcttatcatt ttttgtctta actctggatt agttgtatgc acagatatat
     4801 ttataggact tattctatat tttataatat tatttatatc ttcttcactc atatttgtaa
     4861 gtgtgacaaa atttccttgt aaaaaagata atctcgaatc atcatcttta aaataaagag
     4921 tttctctcat accttctggc aattggtcta taaaacaaaa catacactta tttctacagc
     4981 ttttagcttg gtcaattatc ggatttgtaa attcaacacc caaatcttca tcatagtctt
     5041 tttctatctc gtatatgtag acttcgccat cttttttttg tatttctaca tctaaatatt
     5101 catcacttag taagaatcta tactctatta tatcatgtat tggctgttca ttcacagata
     5161 taagtaaatc tcctacctct atacccattt cttctgcaat actatcttta taaaccttac
     5221 ttataatatt atttactttt tttacattta cttccaatat tttcaccact cttttgtatt
     5281 catcaatgct tattataatg tattttaata tgcaatttat ttatttatta tattttatga
     5341 tagcattttt aagcatttca tgtcataata acacatttta attctaatag tcaatataag
     5401 taaatagcta taaaatagat tagaacaaat aatattcaaa tttaagtttc ttaaaaattt
     5461 aaatattatt tgttcttaaa aaaattttat aaatttaatg ttttactatg attaacgtcc
     5521 catttgtcat atcatgaacc attccaccta cacactttga catcaacaat gccttatttt
     5581 gtaagtcttt ctcatcattt gcataaaata ttggacaacc tccaccattt ttcatattct
     5641 tatcaagtgt aactatagct agggtatatt catttaaacc tatatccatt cccatctcat
     5701 gcacttcctt tctttcctat tttataattt tttagtgata agttttttcc tttagagctt
     5761 gaaagtattg ggcatgactt tacagcttcc attacttttt ccacattctt atctactggt
     5821 aaataagcaa tcattatact ttcatcctct ttatttcttc taggtaatgg tacgaatgaa
     5881 ggttctccat aatctccata taaacctaac cttgaatata tattgtatac tattgcttgt
     5941 ctttgaccgg agtcaaataa tattcctgca ttagcataat ccttattttt aggaattatt
     6001 tgtattccta cacctctatt aatatatact tctctatcgc tttttagacc tatatttgtt
     6061 atacctttta aatctcccac ctttagtata gattcgtcca caaaactaat ttcagcaggc
     6121 attacatcag ctatgtctcc aatacttttt ctagtaagaa gtttttttaa tataaaggct
     6181 aacactaatc cacaaattga acttattacc aaactcatcc cttcacttat tttcgtttct
     6241 gcaagtatta cataatacaa tcctattgtt aaaaaagatg taactataca catataattt
     6301 cttacttcat aggttctagc tatctcttct atgaatgcag ctcctctctt tacaagttga
     6361 acttcctcta aattttgcaa tgtatttctt ctatttgctc tcacttgtct aaattgttct
     6421 gctgctagag ataaaaaagt tatagatgta taagaacgat ttataagagc tggaaccaat
     6481 aatgctccta atgaagaagc gacgaatgct aatataagtt gagatagtaa aacatttggt
     6541 tgcgttggat actgtttttg gtctaaattc aacattataa ttctagatgt tataccaata
     6601 ataacagcaa caaaaaatac tctatccatt aaatcactcc taaaatattt aatttgaaat
     6661 tttacatgta aatctacaaa gtctttctag aaaaatcact ctgctttcct tttgatgttt
     6721 ctatgatagg tgtacttttc actacatcta tcatgacatc tatatctttg agtattggca
     6781 tatatggtat aaccacagta cttttttcta aattggtctt agatatagca acaatatcaa
     6841 cctcatctac atctctatct atccccatat gaataaatag attatgtatt atagcttgtc
     6901 tttgccctat atcatttact attccaaatg cacctaaatc tcttggtata acttctatgg
     6961 ccagacattc atttctatac ttttctcttg tactttctag acctatgtta gttattatta
     7021 caccatttac cattaaaata ggtccttcaa aacttatttt tgcaggaact atatctgcta
     7081 tatctgctat actgttcctt cttaaaaatc ttctaaaaat caaaccaaca atagctcctg
     7141 acacaatagc taggattgtg caataaaaaa agctaaggcc atactttctt gcaaaaacga
     7201 tataaactat cgaagatacc agtgcagaaa aaagacttat ataacttcta gattcatacg
     7261 tagatgctat ttcttcaacg taagcgtctc cttttggtac taattcttca ttatcgatat
     7321 tctttagtgt tattctttct tgttctgcaa gaccttgaaa ctgttgtatt gctactgcaa
     7381 aaaatgtcaa ggctgcaaac tccttatcta taagtgcagg taatgctata gcacctaacg
     7441 aagctgaaag cccagatata attatctgtt ctaaataatc ttgtggtctt gatgggtatt
     7501 gtttgtctgt aactcttaaa actaatcctc tacataatat tcctacaact aatgcaacta
     7561 taaaagagtg tctaaataaa tttgcattca ataaactttc ttccattttc tcagctcctt
     7621 ctcttatata gtttgtgcaa aaaacaaaaa aaattccctt ctatttaagg aaattttttt
     7681 gtaatattgt aactataaat tcgctaccaa tgccaagttc actttttact tttatacatc
     7741 catttagtga tttgactata tgctttgtta tagccaatcc taaacccgtt cctcctacat
     7801 ccctacttct tgctttatct accctataaa atcgttcaaa tatgcgttct acatcttctt
     7861 ttggtatccc tacaccattg tctataactt taattacgat tttttcttca tcttttaaca
     7921 ctacaacttg tacaatccca ttatcttggg tatactttat agcattatca actaggttta
     7981 agaacacttg ctttatatag tctttattgg atagtatact tattgattta tcatcacatt
     8041 tatataaaac ttctatattt ttttcactag ctgaatgttg agttatttca tatacctctt
     8101 taaatacatc atatactaca acattttcca taagaagagt atctttattt tctacaaatg
     8161 ataacagcaa tatatcatca atcaaccttt ttaatctatc tgattcgctt tctattattt
     8221 ctaagaatct atttctagta ttcacatcta tattttcatt tgactttagt gtttcaacaa
     8281 acccacttat agatgtaagt ggtgttttaa gttcatgact tacatttgca acaaaatcac
     8341 ttctcatatt ttcaagttta acttttttag taatatcttc tatatttact atcgaaccta
     8401 ttattacgtt gttagtattc tgaagataaa ctgggtctac ttttatctta tatacgatat
     8461 catctttagt agtaatatta gcagtttcat tatctttaga accaataaat tgaagtattt
     8521 gttttaatat ttcttcttct tttattacaa cattaatatt atgaccttca actaattgat
     8581 ttggttcact ctttatcatt tcttttgctt catcatttat cagaagtata tttccattta
     8641 catctatagc taaaattcca tgagatatac tctttaatat agaccttaac tgtaaatgtt
     8701 tatactgtac ttcttctaag gttttatcca tattctttat catatgatta aaatttctag
     8761 ctaattcgcc aagttctcct ttggctgata tgttaagcct agcatgatat tcattattac
     8821 ttactttttt tgagacgtat ataaactctt ttaaatattt tcttaatgtt attgtgtatc
     8881 ttacagctaa tataacaatg gctatggaca acataacaat aaaaaaatat atactattta
     8941 aatcttccac aagataacac tcctaaaata taaccttttt aatctatttt gtatcctaca
     9001 cctcttatag tctctatata tttttccgat ttatcttcat cttctatttt ttttcttaaa
     9061 tatcttatat gaacatctac tgttctagtt tctccatagt attcataccc ccatatttta
     9121 tccaaaagat aatttcttga aagtactttt cctttatttt gaattaataa ttttaacaat
     9181 tcaaattcct taagagttaa ctctatcttt tcagagcctt tggtaacttc atgttttgat
     9241 aaatctagtt ttaaattacc tgtagtaagt atattgtttt cttcacctaa gctagtcaaa
     9301 ttatatctcc ttaaaacaga actaatccta gctaacaact cttttatact aaatggctta
     9361 gttatatagt catcagcacc tatttcaagc ccttcaacct tgtcattttc catattttta
     9421 gcagtaagca ttataacagg aatatttttt aagtccttat cacttcttat ttttctcaaa
     9481 acttctattc cacttatatt tggtaacatc caatctaaaa gtattaaatc tggtttaatt
     9541 tccttagcct ttataaatcc gtcaaatcca tcatatgaat aactaacttc gtaatttgac
     9601 acctccaaat tgaattttaa tagctcaact atatgcattt catcgtctat aacaagcact
     9661 ttagtattca ttaaaaagct cctctctatt ttatctgtag taccgttcct attcgttctg
     9721 atgccttatt atgttttcta taagagtgga atttatctgc tctacaacta gtgcataaat
     9781 ttaaatttat tatattttct tttttaactc cactacatct aagcatatac tcattaattt
     9841 tccataaatc taaatagtaa cttccctctt ttattatata gaacttttca tcattatttg
     9901 taagaattgt gttaaatttt tctactaaat cttcagacac ttcataacaa catggtccaa
     9961 ttgatggtcc aattatacat actaaatctt caggaactgt tttatagcat tcagacatta
    10021 attttattgt tttatgacct atattactaa aagtccctct ccaacctgca tgagccagtc
    10081 ctatggcttt atttttagca tctataattg atataggtac acagtctgct gtaaacagta
    10141 aaagtggtac tttttccaag tttgttataa gtgcatctcc atctcttctt ttccctatag
    10201 tatctttatt gactatagta actatatctg aatgtatttg agaattacta gttagatttt
    10261 caaatgtcaa atcacattct ttacatactt ttaacatatc ttctctattt tttgcatcta
    10321 tattcttaca tgttataact agatttacag aatttaattt ttcatcaagt tcttcacaat
    10381 taccacttaa attaacatac tctttaatat cttgtacatt ttttatattt atataatctt
    10441 tcatcatttg tctccttttt ctatcagtat acttttaagt tcatttacaa aagtatttat
    10501 atctttaaat tgtctataca cagaagcaaa tcttacatag gcaacctcat ctaactcttt
    10561 caatttatcc attaccatct caccgatttt tttggtttca atttcttcta taaaacattt
    10621 atttatttca ttttcaatat gtacaacaat actttcaatc tcgtcaatag aaacaggtct
    10681 tttttcacat gcttttagca atccatattt tatcttttct ctatcaaaat attctctact
    10741 attatccttt ttaatgacca ttaaaggagt tgtttctatt ttctcatacg tagtaaacct
    10801 ctttttacat gattcacact cacgtcttct tctgatagat tttaaatctg tatgtctaga
    10861 atctattacc tttgattctt tataattaca ataaggacat tgcatctata ctcctccttt
    10921 gtttaactat atctcaaact catctaagct taattctgaa cctatattta ctattattgt
    10981 atcacaacct attttaagta tatcattcca aaatataact tcttcatccc ctcctcttga
    11041 aaaaaaactt ctacttccat ctccaaaaat ggtaaatgag actaccttac cttcgtgaat
    11101 atccatgtct atgtctatta taaaccccat cctttttcca ttttttacat ttataatttc
    11161 tttttccatt atatcagaaa ctctaatcat attgtactca ccctttcttt aaaaataaat
    11221 atgtatcgaa gtaaaaaaaa atgacctata taggtcattt aaattaaaat aatgtttaca
    11281 gtccatggat tacttataca tattttctca tattttttaa agcatttttt tcaattcttg
    11341 atacttgtgc ttgagatata ccgatttcat cagcaacttc tatctgagtt cttcctttgt
    11401 aaaaccttaa atctaatacc aacttttctc tactatttaa tttcttaata gcttctttaa
    11461 gtgatatttc ttgcaaccaa ttttcatcag tatctttttt atcttgaacc tgatccataa
    11521 cgaatattgc atctccattg tcttgatata ctgggtcaaa cagagatatt gggtcttgta
    11581 tggcatcaag tgccattaca actgattcta cttctaattc taattccttt gctatttctg
    11641 atactgtagg ttctttagaa tttgttctaa ttaatctttc tctaacttgt agtgccttat
    11701 atgctatatc ttttaatgac ctacttactc taattgggtt attgtctctt agataccttc
    11761 taatttctcc tataatcata ggaacagcat aagttgaaaa ccttacattt tggcttaaat
    11821 caaagttatc tatagctttt ataagaccaa tacaacctat ttgaaataaa tcgtctatat
    11881 tttctcctct gttgttaaac ttttttatta cactcaagac taatcttaaa ttccctctta
    11941 caaactgttg tctggcttct tcatcaccat tttttatttg taataaaagt tccttcattt
    12001 gcttattttt aagaactgga agttctgatg tattaacacc acatatttca accttattaa
    12061 cttgcatata ttttcagtcc tctctcaaaa gtattcatct attgaaatta tttacatttc
    12121 caattttttt atacttttta agaagaatta atttagcatt atacaaattt tttcatttct
    12181 ttttgcaacc ttgagattat tttcttttca agtctagata tgtaggactg tgatattcca
    12241 agtattccag caacttcttt ttgtgttttt tcaatacctt tatcaataag accaaatctt
    12301 aattccataa tttgcttttc tctgtcactt aatctgtcaa gtgccataac caacaaatct
    12361 ctatctattt cttcttcaat tattttgtat atttcatcat tttctgtacc caatacatca
    12421 gataataaaa gttcgtttcc atctaaatct atatttaaag gttcatcaaa tgatacttct
    12481 gttttctttt tattattttt tcttaagtac attaatattt cattttctat acatctcgaa
    12541 gcataagttg ccaattttat atttttattc agtttaaaag tattaactgc ttttatcaaa
    12601 cctatagttc ctattgaaat caaatcttct acatctatac ctgtattttc aaattttctc
    12661 gatatgtaga ctactaatct aagatttctt tctattaaaa ttgactttac actttcatct
    12721 gtttctagct tttgtaaaag ctccatttct tcttctggtt tcaatggagg aggaagtata
    12781 tttgcccctc ccatatagta tattcccttt ggctttatta tttttaattt aatacctaac
    12841 atagtaataa agcttattat tctttctttc aatcgtaaca ttccttgtcc cccttaaatt
    12901 ttcacctaaa atccaggcaa tatatttgga ttcattattg ctccatattc tctatcatta
    12961 aaatttgaaa taccaagtat tatatttcct atcttttgct catctacttc tatatagtca
    13021 gcttttaagc ctattattat gcttgtctta ttacttccag catgtttata aggtattatt
    13081 ctaactctac ctgaagtttt atcatctaaa cttcctataa tttcttgtgc tttcttcata
    13141 tctatatctt cataattaat atttaatagt ttttctggaa taaatccttc taacacacta
    13201 gacttaacta ttattacatc actttgactt attgggtctt ttaacaaatt accactatct
    13261 actaaagcct tacagcaaaa acttttttct agtaagttta tatttatagt ctttgtaagc
    13321 tccttaatgt acttaagcat tttcaaatct ttataaatgt attttaataa ttcacaactc
    13381 acataagtac atgcaattaa gaaagatatt tttaagtgac ttattcctgt aaaataaatt
    13441 ataaaataag tgcttccaga aataaaaatg tttactagat aaaaaactaa cgctacatga
    13501 acataatctt ttttgctctt acttctaaat gaaattaaag ttattatact catgattatt
    13561 attttaaatg gtacagtaaa tagtatattc attgatggat acaaataagc aacagaatag
    13621 gcacctccta aactagcccc caataactta ttttttttag gattatatct tttagtaaga
    13681 atagaagtac aacttataat aatataattt attactaggt tttctataac atagtactca
    13741 atatacacca tgtatccccc cttaaaacct ttaaacatat tataagatag tatgtacaaa
    13801 attttatgtc acttttgatg gtcgaatcca aaacaaattc tatttaaaaa gaacaatgat
    13861 atatgtctta tactttatgt cgtcaaaaga tattttttta atgccaaaca aatagacttt
    13921 tttgtagaat ccaatatatc taggtagaag ttattcgctc tacttttggt atatttacaa
    13981 aaaaagacta cataatttaa taatatatca agtaaatctt ttacttatat aaaatcaaat
    14041 tatatagtct gtaaattcaa atataaatta ttgtgctagt aatccatttg catattttgc
    14101 agattcattt agtgcttgtt ctggagttat gtctccagct atcatcattt gaagttgttt
    14161 ccataaatat tcatatatct ctactccatg aggtccaact gtcaatggtc tatacatatg
    14221 tttatctctt gtcaccattt cttcaaatct tgggtctcct tggtattttt cacctttagc
    14281 aactggagct ctaggtattt ctttgtggaa agcttccatt gattctttgc ttagcatata
    14341 ctcaagaagt ttaaatccaa gttctttatt tttacatgct gacattaatg ttaaatcatc
    14401 tactgaagca aaacttccaa ctgtcttatc ttctaatgat gttacatatc cccagtttaa
    14461 atccgggaat gatttatcaa aagtacttgt agcagcagag gatagcatta tagtaaatgc
    14521 agcttttcct ggtccaaata cactttcaat catttcatta ctatcttttg ataaagcatc
    14581 ttctggaata tactttttca tatcatttat aaagtttgca gctttcaagc cttctgcatt
    14641 attaaattta actgatttta aatcatcatt atatatatct ccaccagctt gccataaata
    14701 cggataccag ttccagttta agcttccaaa tactttagaa ccccaacctt gtgcaagacc
    14761 ccactggtcg atttttccat ctccatcagt atcttttgtt gctttttcac agattctttt
    14821 aaaatcatcc catgtttttg gaggtttttc tcctaatttt tctagtatat ctttattgta
    14881 gtacaatacg cctggattag cagcttctat aggtaaacca taaataccac ccatcatttt
    14941 tgcatcatct aagtatagat aattatctga agttcctgat ttttctaaat aaggtgttaa
    15001 atcttctacc gcacccattt ctataaattg aggaaacatc tctgcataca tgtatcctat
    15061 atctggacct tcacctgcac ttattgctgt tgcatatttt tctgcgtagt tatcccatgg
    15121 tactatctga aattcaattt cacagttatt ttctttttca aatttttcaa atatcggctt
    15181 ccatgcatct gcatcatggt ctgttaatgg tgcagaccat acaactaatt tttctttacc
    15241 ctctttgcct ttagaatctt ctttttttga acatcctgca agaattgata ctgatagaat
    15301 ccctaccata actaaagaaa gaaccttttt tagtttaatc atgcttaatc ccctttcaga
    15361 ctttaatttg tctttttcct caaatgttta catttaattc agatttaata ttaacaaata
    15421 agattactct tgtctaatca taaaaaacta tactaactcc tataactata ttttttttct
    15481 atacctatta gatttaattc atactaaaaa cttacttttt aaattttatc aatatttaaa
    15541 aatattttta agtctagcca caaactaact atctaaattg aacaaaataa aaagaagtct
    15601 attgacttct ttataagctt acatattatc ttcttcttct taggaatgta ggtatctcca
    15661 tgctctcatc atcatcttct tcaattatac ttgccttttt aggaggctct attttttcct
    15721 cagcaattac ttcttcttca atttctttta cagcttgttt tactgttgta tttaatgaag
    15781 aacgtatgct tgatttaggt ttagtatcta aatctaaggc accattttgt aatccttcaa
    15841 atcctgtcgc gattactgta atcattattt catcacctaa atcttctttt atagaagctc
    15901 caaatataac atttgcttct gggtcacagg attccataac taaagttgat gcctcattaa
    15961 cttcaaataa tcctaagtta ggaccacctg taacattaag aagtactcct ttagctcctt
    16021 gtattgatgt ttctagaagt ggagattgaa tagcttgtct tgcagcatca attgctctag
    16081 tttcaccact agcactacct atacccatat gagcaagtcc cttgtctttc attatagtag
    16141 taacatctgc aaagtccaag tttattaacc cttcaactgc tataaggtct gaaattgatt
    16201 gtataccttg ttttaataca tcatctgcaa ctgcaaaagc atctaacatt gatgtatttt
    16261 tttgtactat ctgtaaaagt ctatcatttg gtatcgttat aagagtatct acttttgatt
    16321 taagttctgc tattccacct tcagcgttct tcattcttat cttaccttca aatgcaaaag
    16381 gtttggtaac tacacctact gtaagtatcc ccatttcttt tgccaagcct gcaaccactg
    16441 gagcagctcc tgtaccagta ccaccgccca ttccggctgt tacaaagacc atatcagctc
    16501 cttgaagtaa ctttactatt tcatctttac tctcctcagc agctcttttc cctacttctg
    16561 gatttgctcc tgctccaagt cctctagtta atttttctcc tatttgaact ttatattcag
    16621 cttttgatgt atataaagct tgtttatctg tatttactga tataaattca acgcctttta
    16681 gttgagcttc taccattcta ttgacagcat tgtttccacc gccacctaca cctattactt
    16741 taatttgtgc acattcttct aattctacgt caaagtttag catcataaac ccccttctct
    16801 catgattcaa agttatcgta tgtattaatt atatcatttt cttcttttat tttgttaacc
    16861 tttaagtaac atattccaca aaaacgtaaa ttttcctttt taaatttctt acttatattg
    16921 tcaaatatta ctctttaagt ataaaataat ttacgtatat attaaatgct ctttaatttt
    16981 tacgtttttt gttcataata ttactaataa tatatcgtct aattatagat aagttattaa
    17041 acaatctcat accaaaaact aatacagcaa catagtaaag agaaactcct aacttttctc
    17101 ctatataagt caataatata gccaatatag cattacctat gaaaccagta ataaaaatta
    17161 cattatcata gttatcttca aaattggatt ttatcgctcc aaaaatagaa tccattattg
    17221 caagaatagc tactgacata tatatggaat aatccataga atatgtaaat ggaatatagt
    17281 aacctactac tacaccaaat gctaatcctc ctaaaaccca taacataaat cttcacctct
    17341 ttattttact ttgaaacaca actacattac aatccttttt attaattttt tttacttttt
    17401 ctttattttt ttgtattttt cgttttattt tgcaaataaa ttcactttat ttccattatt
    17461 ttcctgaact tttatattaa ttccatatat atccttaaga gatttagcat atgattctgg
    17521 agagtttata gctgcactca ataaatctat atctcctata gcttttatta taaatggttg
    17581 tccatatgta gtatcattta ctgtaatagt agctcctgaa cattttatct tacttgttgc
    17641 tagtactctt tctccattta tagaaattgc tcttgctcca gcctttttta aatcatttaa
    17701 aaccctcaaa atatctatat catgtataat caaatcgttt ggattctgtc cgtcttttaa
    17761 ctctctctca ctatccctca tagtaatagt tatgcctggc cctgtaacag ttgaataacc
    17821 acttaactct ttaacttctt caagctcttt ttgcataagt gtttggatag attcatttcc
    17881 attctttttt gcttctttat attcattcaa agtttttttg ttctttttaa gagtttcttt
    17941 taaatttttt atttcttttt ttgtattttc tacctcactt tcaatacttt cagtttgacc
    18001 taatgttatg tatactctat ttgggtctag tgattttata taagttgata tgaaaacgcc
    18061 taatatgaat gctcctaata taactatttt ctttttcata ctattatttc ccctctgctt
    18121 tgtttatatc actatgacca tttatttcta tattgtcttc tttggtcact tttactttgg
    18181 cattataata tgtttctatt tcccagacaa taccatactt tatattaagt gccgaagcca
    18241 atgtatcaga atcaccaatt gcttttatta taattggttc ttttatgaca gtgtcattca
    18301 tataaagctt gtcttctttt aaatacatat atgtattaga aactattctc tggtcgttta
    18361 tagatatggc acttgcttgt gctgaattta atttatttat cattgacaat aataaatcat
    18421 aattatatag tatactatca tttttatcag agtttcctaa gtcttttgta gaatttatct
    18481 caatttttat gcctgttcct tcaacatctg tatatccagc taatgtttca tatttttcta
    18541 tttctgactt aagtatgtct tgttctcctt cattgttttt gcctctataa gtctctatat
    18601 ctttcttcac actattaact tctgattgta gattctcttt ttcttttttt aaagatttaa
    18661 gttcttctgt aagttgctct cctcttttaa aagttgtcat ccctctattt tctatattaa
    18721 taattttaag ttgtaaacta acaagaacac ccaatacaat acttcctatg attatacttt
    18781 tatttataaa tctttttttc aatatatcac ttccttacaa ttgtctatcc ttctggacta
    18841 tacactgcca actttccata gttaagattt atagtccctc cattagtatt tttattttgc
    18901 aggtcaatca gaattttact taccctagat atattataat ctaagttatc atcattagaa
    18961 agtaagattt ttatattact tcttgtaagc atttctatat tgctttcttt ttttaagttt
    19021 acataattta tttttctata aattccttct tcttttaaat aatttaaagt tttaaataca
    19081 ttctccttag ttttataatt tttaaatttt atagatttat tatcatcaat gctataatct
    19141 acatctacta tcaagtcttt tttactttca ttagaacctc ttagttcttc caataaattt
    19201 ccttttttat ctatataaca ataattatct tcatctttta atactgcaaa tatttctttt
    19261 tcttttaaac ttataattat cttattggga agttttcttt ttatttccac attttctata
    19321 tatggatttt ttataagttt atttttcata tctttaaaat tgtatgcaaa aatattttca
    19381 tttagattta catttagttc tttcattata tttgatttgg tcaccctctt atttccaatt
    19441 acatctattt tctttacatc aaataaatca ctttttacaa ttatacatat acaaaaagat
    19501 aacataagta taaaaactaa aactatcatg acattattcg tatttaactt tcttcttttt
    19561 ttcaaatata atcaacccct ttggttaata aatacacctt tagagcttgt ttttcctaca
    19621 aaaaaattat aatatttaaa gtcaaatttt tgcattt
//
LOCUS       DAELLV010000048        19439 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.048, whole genome shotgun sequence.
ACCESSION   DAELLV010000048 DAELLV010000000
VERSION     DAELLV010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19439
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.048"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(64..1254)
                     /locus_tag="KPD58_001451"
     CDS             complement(64..1254)
                     /locus_tag="KPD58_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBG0265229.1"
                     /translation="MNFNQKRIAASIMATAIIMPTMGNLAYANESEVESVSIESRTIT
                     GNAVNFRKGPGTNHESMGKLYKGDKVEYVGKEGSWVKVKYNGNTGYVHGNYVAINSLG
                     SSNESSDTSVKSTKVVTAKGLNFRTGPSTSSSKISTLGYGTEVGYISESNGWSKISSN
                     GRVGYVSSKYLGTSVNDSTNENSENSSNDLVKGTKVVTAKSLNVRTGPGTSHSKIATL
                     SYGTEVGSISESGGWTKISYGNQTGYVSSQYLAEKGSVDTSIPSYSTNSPSQGADSVI
                     SFAKTLLGKPYVWGAEGPNSFDCSGFTQYVMKKSVGVSIPRVSRDQSKYGTYVNRGDL
                     RSGDLVFFDTQGSNNGSVSHVGIYIGNGDMIHASSGSSKKVTISNINSSYYSSRYVNA
                     RRVL"
     gene            complement(1514..1945)
                     /gene="dut"
                     /locus_tag="KPD58_001452"
     CDS             complement(1514..1945)
                     /gene="dut"
                     /locus_tag="KPD58_001452"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBG0265230.1"
                     /translation="MYNLKVKKLNDDAIIPNFAHKGDAGMDLYSIEEVVIPPGETKLI
                     KTGICIELPTMTEAQVRPRSGLALKHSVTVLNTPGTIDEGYRGELKIILINHGKNDFK
                     VEKHMKIAQMIVKPIYDINIEEVKELSDSERGKGGFGSTGF"
     gene            2058..4286
                     /locus_tag="KPD58_001453"
     CDS             2058..4286
                     /locus_tag="KPD58_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HBG0265231.1"
                     /translation="MKLVLAEKPSVAKTIASFLGAKTRQDGYFEGNDYIITYAVGHLV
                     SLYDMKDYDKDKYSGSWKMNNFPFVPEDKFKFKIDSSKTKQFNTVKKLLNRKDVEYVI
                     NATDNDREGELIAFLIFLLAKNKKPVKRILVNEWTPEDITRGIKNLKDEDEMRNLQAA
                     GYTRLITDWLIGINFTSVATLKYGNGKLLNIGRVILPTVKLVYDRDMEILNFVPKTYY
                     EIEGHFKAEAGEYKGKYVKGKESKFDTLEDANKIIASITSETGKILDKKVTMSKEYAP
                     KLFSLTSLQGYITSKYSNFTSDKVLNVCQSLYEGNGKGGYITYPRTDSIFLEESLASK
                     ASQTLDKLKVGLEYENKIKFSKTKRVFDSSKVDSHSAIIPTYIIPKSLSKDEQLVYDA
                     IKDRFVANFMPPAEYENTEIKTEVDNCTFLTKGKVLKSKGYLEVYNKEEKNDLLPLVN
                     KDDVVDVLEIKPLTKQTTPPKPYTEDTLLKAMKNCGKNVPEEDTTVLSGYSIGTSATR
                     ADVLKKISQVGYVKKKGKSYSITDLGKNLVEIFPVKDLFDVDYTGKLEKSLSDIQKGK
                     YTRKEYLTNIISFIYQNVNIIKQDASKNINTEAYTFDSKTKKFTKTKEKKQKKDVTNT
                     EKEVAISKDSNNKKEVKNGDENQSLGKCPICQGDVLEFDKGFACKNHKECKFVIWKND
                     KYLSLYKKKVNKTMVKNILKKGETKVKSLTAKNGNKFDATLKYDKNPDTGYFNWKIEF
                     DN"
     gene            complement(4397..4882)
                     /locus_tag="KPD58_001454"
     CDS             complement(4397..4882)
                     /locus_tag="KPD58_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0265232.1"
                     /translation="MDNNNILKIADSFLDFLFVLENNIFKESDLLKKFQNNSDIMKAY
                     FEECPMAPSHAKVILYLMTSNSSSISQIASNLGILKSNMTPIIDRLVEHGLVNKFPDP
                     KDRRILRVELTDKAFKLFDAVKAILKESLVKKLSNLSEEELTLLDEHTLKLSEIVKKL
                     G"
     gene            5134..6357
                     /locus_tag="KPD58_001455"
     CDS             5134..6357
                     /locus_tag="KPD58_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBG0265233.1"
                     /translation="MKRYLIVFLMCSSLFLVGCGKTEESEPEKPIAVSVQKAIGGEIE
                     NTNSFSGTTKVKDETAVTAQTVGTVQEVYVKLGQNVRKGDELLSISSPELENSVKQSK
                     ASLDLAKASYSSATGGSLEAQVNQAKTALDNAKIQYEESQRNYDNNKILYEQEVISLD
                     QFKKIEFSLEQTKQQLDSAQRAYDTATSKSIPQAKALAKKQLDQAQVSYNLAMSNLDK
                     LTLTSPVDGTITAKNFDSKEMITQSQPAFIISNPNILEVDLNVAESDIGKFKKDGNVD
                     VIIEDQRILGKIDYVPSVVDPQTSLYPVKVLVNNTNNKFKAGMSAQVNLSVEKENGAV
                     TVPKKAIFEENGKKYVYIATKDNIAKKHLVQTGIVTEDKIEIKSGVSDKDTVIIGGIS
                     LISDGTKIFPVEKEK"
     gene            6361..9495
                     /locus_tag="KPD58_001456"
     CDS             6361..9495
                     /locus_tag="KPD58_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HBG0265234.1"
                     /translation="MNLTQTSVKRPLTIIMVFLVLIVFGGIGYKKMSINLMPDIEIPV
                     VMVMTTWTGAGPQDVDEQVSQKVDESLSAVSNVKSTISSSQESVSMVVAQFEFGTNLD
                     EIMNDVRSKVDALQTSLPDDAAKPTVLKLDMNAQAIGQLVISGGNENSSQALRKYAED
                     VIQPKIESIDGVTSADLKGGEKAQVNVIADPAVLSNYGVSLSTIKGVLSSSNKTFPYG
                     SITQGEDKIVLRAIDKLESLDDIKQIQIPVKGGNTVRLDNLCSVDYGYADKDSIYRYN
                     GKDSLVIDISKQQDANTVKVMQSAYKYVDELNKENPQFKIKIANDTSSYISESINSVM
                     SNLLISAVIAFIVIFAFLKSVRASLVVAVAIPISIIGAIAILYFTGESLNLITASSLV
                     ISVGMVVDNATVVIENIFKYRKSGRLSLDDCAIEGTRTVTNAILASTLTTIAIFLPIM
                     FTEGIAKITFGALGKTLIASLAFSFIGAITLVPSVFAKLSRGKNSQKMQEKDSPIFDK
                     VSEAYKKLLSVCLRHQRLVVISSLLIFVASLFGMGFIGMDFMPAADKGELSISIELPK
                     GLSLESNDYYVSMTEKKVSDIPEIKTLITTLTSDSNNSKASIAIELESQKNRKRTTDE
                     IEKEIIDRVATVPDCKINVSQNSSVMGGAGGADFTLEMKGPDLDVLKILGKEAEKEFS
                     SIKGFRNVETSLTDTTQEAQFTIDKHKAQEYGVNTAEIAGMLRTAINGDSVTTATIDD
                     YKVDVNLKFKADTIDSLEDIKQLKVNSATGKEVPLGAFSDIKMADGLQSISRTDGDFS
                     VNITATLDNLDAGTATKLALEKTNKMVIPKDYSIGVGGETEMMNESMASLGFSMVIAI
                     ALVYMVMVAQFESFSKPFIIMFCVPFAFVGVVLSLLVSGQSLSTVGMLGIILLIGIVV
                     NNGIVLIDYIEQLRKTNRNNDLIGLVSIGCAARLRPVLMTTATTILGMLPTALSLGEG
                     GETMQPLAIVIMGGLTVSTLITLVLIPTIYLIFDKWENKFSDKMPKIFGKITDKIGSI
                     KRKIIKKPKLKAKV"
     gene            9529..10827
                     /locus_tag="KPD58_001457"
     CDS             9529..10827
                     /locus_tag="KPD58_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HBG0265235.1"
                     /translation="MSKIVAIGCGVGIITANLIPVYADNNTSSINKVDNSILSDSNKR
                     TSEKEILTAREAVSAALKNSEKLKMKSEEIKMLKEKLEVQDEFDSFTGSDNSFPYDQI
                     GLLKNQSEQAKGFMEDQIANDITNKFNDLVSRENELDKIKNNLEIKTKEIKDMKLKKD
                     LGLVTSLETESAELELQTLQNTQKAKLQELKNNQDYFKLLTNIDLNNYQLDKEYRFES
                     FRVGGSVDSYMEGKVNEYLKYDQLILERTEESFNDKDENKADLPDRPNLTRPVAPTKP
                     EQGNLTDDEYKVLMDKYEKDYDRYKKEVEAYNLERQTYAAGLTTYANYLQQKFNTENG
                     LVTLEDSKKALKKGLIDSYAQLLALEDTIQITKKQLDLSEKQLKNTKLRYDLGLITLT
                     DYKKQVVSNEDAKNSYDSLIVNYNSLKNGIEKPWILNTGK"
     gene            complement(11116..11361)
                     /locus_tag="KPD58_001458"
     CDS             complement(11116..11361)
                     /locus_tag="KPD58_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG0265236.1"
                     /translation="MTKCPKCGKEVSSRPGSICPRCGFKVADESLKIRCPEPSCRALV
                     SRKLDYCPKCGCKLRGYNISEFVNMVRCKIDETFDKK"
     gene            complement(11575..14202)
                     /locus_tag="KPD58_001459"
     CDS             complement(11575..14202)
                     /locus_tag="KPD58_001459"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HBG0265237.1"
                     /translation="METKYVYSFGEGSKDMKSLLGGKGANLAEMTKIGLPVPPGFTIT
                     TEACNDYYVNNESIRAEIIKEIEEHLATLEKDLNKTLGCNKNPLLVSVRSGAVFSMPG
                     MMDTILNLGLNDNSVVGLAEATQNERFAYDSYRRFIQMFSDVAMEVPKYKFENVLDRV
                     KEAKGYTVDTELTTDDLKEIVKEFKAIYKKEIKNDFPQDPKEQLMLAIEAVFRSWNNP
                     RAIVYRKLNDIAHNLGTAVNIQSMVFGNMGETSGTGVAFTRNPATGENKLFGEFLMNA
                     QGEDVVAGIRTPQNISTLADVMPAVFDEFVKITHILEGHYKDMQDIEFTIENERLYIL
                     QTRNGKRTAAAAINVAVDLVEAGIIDEKEAIMRIEPNQLDQLLHPKFEDKALKEAKVI
                     AKGLPASPGAASGKVYFNADDVVKANEKGEKVVLVRLETSPEDIEGMVKAEGILTARG
                     GMTSHAAVVARGMGKCCVAGCGEIKVDEFNKEIRALDDVVIKEGEYISIDGSTGNVYL
                     GDVKKTEVSLTGNFEKLMNWVDKHKCMMVRTNADNPRDARAAIEFGAEGIGLCRTEHM
                     FFDEARLPAVREMILSNTVEQREKALEKILPMQREDFVELFKVMDGKPVNIRLLDPPL
                     HEFLPHDDETIEELSKSMGIKVSDIKKRIVDLDEFNPMLGHRGCRLAITYPEICVMQS
                     KAIIQGAIEAIKAGVKVSPEIMVPLVGEVNELKIIRKMIVETVDAIIKEEGIEVPYTV
                     GTMIEIPRACLTADEIAQEADFFSFGTNDLTQMAFGYSRDDAGKFLGQYVDEEILEKD
                     PFQVLDQNGVGKLVKMGAKLGREVKPELKLGICGEHGGEPSSVEFCYSVGLNYVSCSP
                     FRVPIARLAAAQASIKNPR"
     gene            complement(14263..15105)
                     /locus_tag="KPD58_001460"
     CDS             complement(14263..15105)
                     /locus_tag="KPD58_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HBG0265238.1"
                     /translation="MLVKNLIIYAVSDSVGETAQQVAKACMSQFYVNETYEIKRFPYM
                     INKGVLLETLENAKAENALIVYTLVDEELCSIVERYCEREGLSCIDLMTDILREISKR
                     TGRKPKREAGIIRKLDESYFKRVEAIEFAVKYDDGKDPRGVLQADIILVGISRTSKTP
                     LSMYLANKNIKVANVPLVPEIPIPKEVFEIDTKKIIGLTNSPEKLNEIRTQRLKALGL
                     SSKANYANLERILQELDYSEEIMKRIGCPVINVSNKAIEETAGIILDIMKENGLKIYK
                     EIEI"
     gene            complement(15112..15750)
                     /locus_tag="KPD58_001461"
     CDS             complement(15112..15750)
                     /locus_tag="KPD58_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0265239.1"
                     /translation="MIIIQLNERQLKIIDIVKENEPITSESIASSLNVTRATLRSDLA
                     ILTMTGILDARPKVGYFYSGVSEINLIGKSVKEKTVEDIMSMPVLAKKDESIYDVIVT
                     MFLSDVGSIVIIDENEELCGVVSRKDLLKATIGGSDINKMPIGMIMTRTPNVVTLTKG
                     ASVLLASRKIIEHEVDSIPIVEYKGEDKNHMRVVGRISKTNITKLFLEIVDN"
     gene            complement(16186..17478)
                     /locus_tag="KPD58_001462"
     CDS             complement(16186..17478)
                     /locus_tag="KPD58_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBG0265240.1"
                     /translation="MLDLNRKLAKLEEEGKKIKVALIGAGHMGNGMVSQMANMKGIEA
                     SIVVDINLELAHKAFTDAGIDEEIIDNVTNEKDAEIKLQEGKVLTCNDFLVACKTKSI
                     DVVIDATGGIAIGAEIALNSILNKKHIVMLNAETDCVVGPILKKLADDAGVIFTGSAG
                     DEPGAVMELFDFADAMGFEVRVIGKGKNNKLDLDCNPDTVREEAERKGASPHMIASFK
                     EGTKTMVEMALMCNATGFVPDVRGGHGIEATVNEVPKKYALKSEGGVLDNYGVVDFVN
                     GIAPGVFVVVAHKLKAVNDELKYLSMGDGPNYILYRPYHLCSLETPLSAAMAVLDHKA
                     TIVPKAGLVAEVMTIAKKDLKKGEFMDGYGAYTCYGTIEKYDVAKAMNAVPIGLISKK
                     TKVVKDIKKGEVITYDMVEIEKDTTLYHLRQLQEKIFG"
     gene            complement(17515..17871)
                     /locus_tag="KPD58_001463"
     CDS             complement(17515..17871)
                     /locus_tag="KPD58_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBG0265241.1"
                     /translation="MKYEVKISGIGSLVKELMDESNCLIIYDETINDDDLKDISVIHS
                     IATLKSDVEIGDTLTIGNRDYCIVSVGDIAQKTLREIGHCTIKFDGKCEVNLPGEIHV
                     EIGNPDITIGDLITIS"
     gene            complement(17906..18265)
                     /locus_tag="KPD58_001464"
     CDS             complement(17906..18265)
                     /locus_tag="KPD58_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265242.1"
                     /translation="MKLLVILFVAYILNTIFIMKQNKLYFKTLNRAKKKGDIVSTGKK
                     KSYFSKGSIAIISSDSDGFIKFGEILKGRTVMAKFKEIEEIEGLDIYSAEQKFKDEES
                     IVQAIKFIKEKINVSFN"
     gene            complement(18334..>19439)
                     /locus_tag="KPD58_001465"
     CDS             complement(18334..>19439)
                     /locus_tag="KPD58_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG0265243.1"
                     /translation="ASECALNSSRISKNIDELMENIFNQIYVDMGIFINEDELRVGLG
                     YHLSFTLNRLLFNIKLKNVLLEEIKQNCILPFKLAQIAGKVIEKKYNLEVSEDEIGYI
                     AIHFSGYLERNSSRFYSIKKIAIICSTGLGTAKLLKIRVEKLIGNNPKIDTLSSFNLR
                     NINLDEYDIVFTTIDLDTSNINTIVLKINTIFDENKLREQLKTVLCLREGNIDTTNST
                     NLLINNLLDEDKFMILNEKTILASLEKMMDHLMDLGCIDEKFRKNIVNREEKSPTVFD
                     KGLLFPHSINEKSDKFLMAVGILEEPIEYANRSIKIILMTMFPCENKIDSDLLVKIYE
                     EVLKIGQDVKLTNKISKCRTFLEFKKVLLKNLI"
BASE COUNT         6700 a         3184 c         2597 g         6958 t
ORIGIN      
        1 acctgctttc atattataag caataaagaa aaatctattt attaggtttt ataataaaaa
       61 caattataaa actcttcttg catttacata tctacttgaa tagtagctac tatttatatt
      121 agatattgtt actttttttg atgaacctga agatgcatgt atcatatctc catttcctat
      181 gtatattcca acatgactta cagaaccatt atttgaacct tgtgtatcaa agaatactaa
      241 atctccactt ctaaggtctc ctctatttac atatgttcca tacttacttt ggtctctaga
      301 tactcttggt atactaactc ctacagattt tttcataacg tactgcgtga atccagaaca
      361 gtcaaaacta tttggacctt ctgctcccca tacatatggt tttccaagta gtgtctttgc
      421 aaaacttatt acactatcag ctccttgact tggagaatta gtcgaataag atggaattga
      481 agtatcaaca gaaccttttt cagcaaggta ttgacttgac acatatccag tttgattgcc
      541 ataacttatt tttgtccatc ctccactttc agaaatactt ccaacctcag taccataact
      601 taatgtagct attttagaat gacttgtacc tggtcctgtt ctaacgttca atgactttgc
      661 tgttacaacc tttgtaccct ttacaaggtc atttgaactg ttttcagaat tttcatttgt
      721 agagtcattt acactagttc ctaaatattt acttgaaaca taacctactc taccattaga
      781 acttattttt gaccatccat ttgactcaga tatgtatccg acttcagtcc catatcctag
      841 tgttgatatt ttagaactac ttgtacttgg tcctgttcta aaatttaatc cttttgctgt
      901 tacaactttt gtcgacttaa ctgaagtatc actactttca ttgctacttc caaggctatt
      961 tattgctaca taatttccat gcacataacc tgtgtttcca ttatatttaa cttttaccca
     1021 agaaccttct tttcctacgt actcaacttt atcacctttg taaagtttcc ccattgattc
     1081 atggtttgta cctggtcctt ttctaaaatt taccgcattc cctgttattg ttctgctttc
     1141 tatactcaca ctctctactt cagactcatt agcatatgct aaattcccca tagtaggcat
     1201 tattatcgca gttgccatta tactggctgc tattctcttt tgattaaaat tcataacttt
     1261 ccactcatct cctcttttct actatcttta tttcacttca aaatataaaa gaaataattg
     1321 acactatcaa aatatcaatt atttctttta tattaatatt attacaaatt tgtaactatt
     1381 tgtctatagt aatatttgct taccataaat taccatgtat aaattttaat ctttaatagc
     1441 ctgtaattta gtaatatagc catcttttac cttttttaaa aatggctata ttttttaact
     1501 tttttgtaac tttttaaaat ccagttgagc caaacccacc ttttcctctt tcggagtcac
     1561 ttaattcttt tacttcttct atgttaatat catagatagg ttttactatc atttgagcaa
     1621 ttttcatatg cttttcaacc ttaaaatcat ttttaccatg atttattaat ataattttaa
     1681 gctctcctct gtatccttcg tcaatagttc ctggtgtatt taatacagta acagaatgct
     1741 ttaaggcaag cccacttcta ggtcttacct gcgcttctgt catagtagga agctctatac
     1801 atatcccagt tttgataagt ttagtctctc caggaggtat tacaacctct tctatagaat
     1861 acaaatccat accagcatca cctttgtgag caaaattagg tattatagca tcatcattta
     1921 atttttttac ttttaaattg tacatcttat atctcctttc aactttctat aaatattata
     1981 acttaaattt cgacctcttg aagtttttta tttttttgtg gtaatataat tattagtgtt
     2041 ttaaaggagg attttttatg aaacttgtac ttgctgagaa accatctgtg gcaaaaacta
     2101 tagcatcatt tttaggtgca aagaccagac aagatggtta ctttgaagga aatgattata
     2161 taattactta tgcagttgga cacttagtat cattatatga tatgaaggat tatgataaag
     2221 ataaatactc tggctcttgg aaaatgaaca atttcccatt tgttcctgag gataagttta
     2281 aatttaagat tgatagttct aaaactaaac aattcaacac tgtaaaaaag cttttgaata
     2341 gaaaagatgt agaatatgta ataaatgcca ctgataatga ccgagaagga gaacttatag
     2401 catttttaat atttttactg gcaaaaaata aaaaaccagt aaaaagaatc ttggtaaatg
     2461 aatggacacc agaagatatt acaagaggta taaaaaacct aaaagatgaa gatgaaatga
     2521 gaaatcttca agctgctgga tatactaggc ttattactga ctggttgatt ggaataaatt
     2581 tcacttctgt tgctacattg aaatacggaa atgggaaact attaaacata ggtagggtaa
     2641 ttcttccaac agttaaatta gtctatgata gagatatgga aatactaaac tttgttccta
     2701 aaacttatta tgaaatagaa gggcatttta aagctgaagc tggtgaatat aaaggtaaat
     2761 atgttaaggg taaagaaagt aaatttgata cacttgaaga tgcaaataaa attatagctt
     2821 caattacatc tgaaactggt aaaatactag ataaaaaagt tactatgtca aaggaatatg
     2881 caccaaaact ttttagtttg acatcacttc aaggatatat aacttctaag tattccaatt
     2941 ttacatctga taaggtttta aatgtctgtc aatctctcta tgagggtaat ggtaagggtg
     3001 gatacataac ttaccctaga actgattcca ttttcttaga agaaagtttg gcttcaaaag
     3061 cttctcaaac tttagataaa ttaaaggttg gtcttgaata tgaaaataaa attaaatttt
     3121 ctaaaactaa aagagttttt gattcttcta aagtagactc ccatagtgct ataattccaa
     3181 cttatataat tccaaaaagt ttatctaagg atgaacaatt agtttatgat gccataaaag
     3241 atagatttgt agcaaacttt atgcctcctg ctgaatatga gaatacagaa attaagactg
     3301 aagtagataa ttgtactttc ttaacaaaag gtaaggtgct taaatcaaaa ggataccttg
     3361 aggtatataa taaggaagaa aaaaacgatt tacttccact tgtaaataaa gatgatgttg
     3421 tagatgtgtt agaaataaaa ccattaacta agcaaactac tccacctaaa ccgtatactg
     3481 aggacacact tttaaaggct atgaaaaatt gtggtaaaaa tgtgcctgaa gaagatacaa
     3541 cagtgctatc tggatattcg attggaacat cagccacacg tgctgatgtg ttaaagaaga
     3601 taagtcaagt gggctatgta aagaaaaaag gtaaatctta ttctataaca gaccttggga
     3661 aaaatttagt tgaaattttt cctgttaaag acttatttga tgtagattat actggaaaac
     3721 ttgaaaaaag tttaagcgat attcaaaaag gaaagtatac aagaaaagaa tatcttacta
     3781 atatcattag ttttatatat caaaatgtca atattataaa acaagatgct tcaaaaaata
     3841 taaacacaga agcatatact ttcgattcaa aaactaaaaa atttactaaa acaaaagaaa
     3901 aaaaacagaa aaaagatgta actaatactg aaaaagaagt tgctatatca aaggattcta
     3961 ataacaaaaa agaagttaaa aatggtgatg aaaatcaatc tcttggcaaa tgccccattt
     4021 gtcaaggtga tgtactagaa tttgacaaag gctttgcttg taaaaatcat aaagaatgta
     4081 aatttgttat ttggaaaaat gataagtatt taagtcttta taaaaagaaa gttaacaaaa
     4141 caatggtcaa aaatatttta aaaaaaggtg aaactaaagt aaaatcttta actgctaaaa
     4201 acggtaataa atttgatgct acattaaaat atgataaaaa tccagatact ggctatttca
     4261 attggaaaat agagtttgac aactaaatat tcataagaaa agaaatagca gtagtataat
     4321 aatctatatt atattactgc tatttctttt cttatgaagt ttcaactttt ttaatagtga
     4381 cttttacaat cacactctaa cctaatttct ttactatttc tgataacttt aatgtatgtt
     4441 catctaataa agttagttcc tcttcagaaa gattagataa tttttttact agagattctt
     4501 tgagaatagc ttttactgca tcaaataatt taaatgcttt atctgttaat tcaaccctaa
     4561 gaatccttct atcttttggg tcaggaaatt tattgactaa accatgttcc actaatctgt
     4621 caataatcgg agtcatattt gattttaaaa ttcctaaatt agaagctatt tgagaaattg
     4681 atgatgaatt tgatgtcata agataaagaa ttaccttagc atgtgatgga gccattggac
     4741 attcttcaaa ataagctttc attatatcag agttattttg aaactttttt aacaaatcgc
     4801 tttctttaaa tatattattt tcaagcacaa ataaaaaatc taaaaaagag tccgctattt
     4861 taagtatatt gttgttatcc atatttgtaa catcccttca atagataata taataaaaca
     4921 ttgttgttta atatagtata aattataaac tattaataaa tttaaaacaa gaaaaagtta
     4981 ttgactcata ataattataa atttataatc aaattagaga attataaatt gccaataaag
     5041 ggaagttttt taattagtct aaaactaaac ttaaattata agacacttta aattctacaa
     5101 caagctagtg tgcaatatat aataggagga gatatgaaaa gatatttaat agttttctta
     5161 atgtgtagtt cactgttttt agttggatgt ggaaaaacag aagaatctga acctgagaaa
     5221 ccaatagcag tttctgttca aaaagcaata ggtggagaaa ttgaaaacac aaattctttc
     5281 tcaggaacta caaaggttaa agatgaaaca gctgttactg cacagactgt tggcacagtt
     5341 caagaagtgt atgtaaaact tgggcaaaat gtaagaaaag gagatgaatt attgagtata
     5401 agcagtccag agctagaaaa tagtgtaaag caaagtaaag cctcactaga tttagcaaaa
     5461 gcaagttatt caagtgccac tggtggttca ttagaggcac aagtaaatca ggcaaaaaca
     5521 gctcttgaca atgcaaagat tcaatatgag gagtctcaaa gaaattatga caataataag
     5581 attctttatg aacaggaagt cattagttta gaccaattta aaaaaataga attttcttta
     5641 gagcaaacaa aacaacagtt agattctgct caaagagcat acgatacagc aacttcaaaa
     5701 agtatacctc aagctaaagc acttgctaaa aaacagcttg accaagctca agtatcatat
     5761 aatttagcaa tgagtaattt agacaaatta acattaacat ctccagtaga tggtacaata
     5821 acagctaaaa actttgattc aaaggaaatg ataacacaat cacaaccagc ttttattatt
     5881 tctaatccta atatactaga agttgattta aatgtagcag aatcagatat aggtaaattt
     5941 aagaaagatg gaaatgtaga tgttataatt gaagaccaaa ggattttagg aaaaatagat
     6001 tatgtacctt cagtagttga ccctcagaca tctttatatc ctgttaaagt acttgtaaat
     6061 aatacaaaca acaaatttaa ggcaggtatg tctgctcaag taaacttaag tgttgaaaaa
     6121 gaaaatggtg ctgttacagt acctaaaaaa gcaatatttg aagaaaatgg taaaaaatat
     6181 gtatacatag caacaaaaga caatatagca aagaaacact tggttcaaac tggaattgta
     6241 acagaggata aaatagaaat taaaagtggt gtaagtgata aagatacagt gattatcgga
     6301 gggataagtc ttatttctga cggaactaag atatttcctg tagaaaagga gaagtagact
     6361 atgaatttaa cacagacttc cgttaaacgt cctctcacaa tcataatggt ctttttagta
     6421 ttaatagttt ttggtggtat tggatataaa aaaatgtcaa taaatctaat gcctgatata
     6481 gaaataccag ttgtaatggt catgactact tggacaggtg ctggtcctca agacgttgat
     6541 gaacaagtta gtcaaaaagt tgatgaaagt ttatctgccg tttcaaatgt aaaatctact
     6601 atatcaagtt cacaagaaag tgtttctatg gtagttgctc agtttgaatt tggaactaat
     6661 ttggatgaaa taatgaatga tgttaggtct aaagttgatg cattacagac cagcctccca
     6721 gatgatgcag ctaaaccaac agttttaaag ctggatatga atgcacaggc tataggtcaa
     6781 cttgtcatat ctggaggaaa tgaaaatagt tcacaagcct taagaaaata cgctgaagat
     6841 gtaattcagc ctaaaataga atccatagat ggtgttactt cagcagacct taaaggtgga
     6901 gaaaaagcac aggttaatgt aatagctgac ccagctgttc tttcaaatta tggagttagt
     6961 ttatccacta taaaaggtgt cttatcctct agtaataaaa cattccctta tggttcaatt
     7021 actcaaggtg aggataaaat agttttaaga gcgattgata aattagaatc cctagatgat
     7081 ataaaacaga ttcaaattcc tgtaaaagga ggaaatacag ttagattaga taatctttgt
     7141 agtgtagatt atggatatgc agacaaagat agtatttata gatacaatgg aaaagacagt
     7201 ttggtaatag atatttctaa acaacaagat gccaacactg taaaagttat gcagagtgca
     7261 tacaaatacg tagatgaatt gaataaggaa aatcctcaat ttaaaattaa aattgctaat
     7321 gatacaagtt catacataag tgaatcaata aacagtgtaa tgagcaactt gcttataagt
     7381 gctgttatcg catttattgt aatatttgct ttcctaaaaa gtgtaagagc ttcactagtt
     7441 gttgcagttg caataccaat ttctataata ggtgctatag caattctcta ctttactggt
     7501 gaaagtttaa atcttattac tgcaagttca ttggtaattt cagttggtat ggttgttgat
     7561 aatgctacag ttgtaattga aaatatattt aaatatagga aaagtggcag attgagtctt
     7621 gatgattgtg ctattgaggg aacaagaact gtaacaaatg caatacttgc atcaacattg
     7681 accacaatag caatattctt acctatcatg tttacagaag gtatagctaa aataactttt
     7741 ggggcattag gtaaaacact aatagcttca ctagcattct cctttatagg tgcaattaca
     7801 ctcgttccta gtgtatttgc aaaattaagc agaggaaaaa atagtcaaaa gatgcaagaa
     7861 aaagatagtc ctatatttga taaggtaagt gaagcatata aaaaattatt aagtgtatgt
     7921 ttgagacatc aacgcttagt tgtaatatca agtttactta tttttgtagc ttccttattt
     7981 ggaatgggat ttataggaat ggactttatg cctgcagcag acaagggtga attatctatt
     8041 tctatagaac ttccaaaagg tcttagccta gaatccaatg attactatgt atctatgact
     8101 gagaaaaaag tctcagatat tccagaaatt aaaactttaa taacaacttt gacatcagat
     8161 tcaaataata gtaaagcaag tatagcaatt gaattagaat ctcaaaaaaa tagaaaaaga
     8221 actactgatg aaatagaaaa agagataatt gatagagtgg ctactgtacc agattgtaaa
     8281 ataaatgtaa gtcaaaacag tagtgttatg ggtggagctg gaggagcaga cttcacatta
     8341 gaaatgaaag gtcctgattt agatgtatta aaaatactgg gaaaagaagc tgaaaaagaa
     8401 tttagctcta taaaaggatt tagaaatgtt gaaacttctc ttacagatac aacacaagaa
     8461 gctcaattta ctatagataa acataaggct caagaatatg gtgtaaatac tgctgaaatt
     8521 gcaggcatgt tacgtacagc aataaatggt gactctgtaa ccactgctac tattgatgat
     8581 tacaaagtgg atgtaaacct aaaatttaaa gcagatacta ttgatagctt agaagatata
     8641 aagcaactaa aagtaaactc tgcaactgga aaagaagttc ctcttggtgc tttttcagat
     8701 ataaaaatgg cagatggtct acaatctata tctaggactg atggagattt ttctgtaaat
     8761 ataactgcta cacttgacaa tttagatgct ggtactgcta caaaactagc actagaaaaa
     8821 acgaataaaa tggtaattcc taaagattac tctataggtg ttggtggtga aacagaaatg
     8881 atgaatgaat ctatggcaag tcttggattt tcaatggtca tagctatagc attagtttat
     8941 atggtaatgg tagctcagtt tgaatcattt agtaaaccat ttataatcat gttctgtgtt
     9001 ccatttgcat tcgttggagt tgtgctttcg cttctagtta gtggtcaaag tttaagtact
     9061 gtcggtatgc taggtataat cttacttatc ggtattgttg ttaataatgg tatagttctt
     9121 attgactaca tagagcaact tagaaaaaca aatcgtaaca atgatttaat tggtttggtt
     9181 tcaattggtt gtgcagcaag attaagacca gtcttaatga ctacagcaac aacaatttta
     9241 ggtatgttgc caacagcact atctcttggt gaaggtggag aaactatgca accactagcc
     9301 atagttataa tgggtggtct aactgtatct acacttataa ctttggtgct tatacctact
     9361 atatacttaa tatttgataa atgggaaaat aagttttcag acaaaatgcc taaaatattt
     9421 ggtaagataa cagataaaat aggaagtata aaacgtaaaa taataaagaa accaaaattg
     9481 aaagctaaag tttaaataga atggagggtg attgtattga ataaaaaaat gagcaaaata
     9541 gttgcaattg gctgtggtgt tggtataata actgccaatt taatcccagt ttacgcagat
     9601 aataatactt cttctataaa caaagttgat aattctattt taagtgactc aaataaaaga
     9661 acatctgaaa aggaaatttt gactgctaga gaagctgtta gtgctgcact taaaaatagt
     9721 gaaaaattaa aaatgaaatc agaagaaata aaaatgttaa aagaaaagtt agaagtacaa
     9781 gatgagtttg atagttttac tggaagtgac aactcgtttc catacgacca aattggactt
     9841 cttaaaaatc aaagtgaaca ggctaaaggc tttatggagg accaaatagc aaatgatatt
     9901 actaataaat ttaacgattt agtttcaaga gaaaatgagc tagataagat aaaaaacaat
     9961 cttgaaataa aaactaaaga aattaaagat atgaaattaa aaaaagattt agggttagta
    10021 acatctttag aaactgaatc agctgaactt gaacttcaaa cactacaaaa tactcaaaaa
    10081 gctaaacttc aagaactaaa aaacaatcaa gactatttta agttattgac taatatagat
    10141 ttaaataatt atcaattgga taaagaatat agatttgaat cttttagagt aggtggctca
    10201 gttgattcat atatggaagg taaggttaat gaatatttaa aatacgacca attgatttta
    10261 gaacgtacag aagaaagctt taatgataaa gatgagaaca aagcagattt accagataga
    10321 cctaacctta caagaccagt tgctcctaca aaaccagagc aaggtaacct aactgatgat
    10381 gaatacaagg ttcttatgga taagtatgaa aaagattatg atagatataa aaaagaagtt
    10441 gaagcataca atcttgaaag acagacttat gctgctggtc ttactactta tgcaaattat
    10501 cttcaacaaa aatttaatac tgaaaatggt ctagtaacat tagaagacag taaaaaagct
    10561 cttaaaaaag ggttaattga tagctatgct caattacttg ctttagaaga tacaattcag
    10621 attactaaaa aacaacttga tttatcagaa aaacaattaa aaaatactaa attaagatat
    10681 gatttaggat taattactct aactgattat aaaaaacaag tggtaagtaa tgaagatgct
    10741 aaaaatagct atgactctct tattgtgaat tataactcac taaaaaatgg tatagaaaaa
    10801 ccttggatac ttaatactgg aaaataattc tggtaaatag aaagattact ttaacatacg
    10861 tttgattaaa aaatagttat gcacactcgt ggctttagtc atggaatatt cagaaggcaa
    10921 ataatagaaa tagattagta aaaatatatt tacttataaa actgtatact tttatagagg
    10981 ctatctcaaa atagagataa gcctcttttt tattgtttaa attataaaaa tatatttgat
    11041 tccatacaaa aagagtgcct tatgaactaa aaagttcatt ttgagacacc ctcaaaagct
    11101 taactctaaa tttaattatt ttttatcaaa tgtctcatct attttacatc tcaccatgtt
    11161 aacaaattca gaaatattat atcctctaag tttgcatcca catttaggac aataatccag
    11221 tttcctagat accaaagccc tacaactagg ttctggacat cttattttta gactctcatc
    11281 tgctacttta aatccacatc gtgggcatat actacctgga cgtgatgata cttcctttcc
    11341 acatttagga cactttgtca actttatccc ctccagtaaa ctcaatttaa ttgatagttg
    11401 aaatttctct ctacaataat atatgcaata taattaaata cgtttcactt acaactatat
    11461 aagttaaatt tttataataa aaatccccta tattcaattt acaaatactt aatttgatac
    11521 attgaacaat aggggatttt atttttattt agttacatat tttgcagtct attattatct
    11581 aggatttttt atagatgctt gagctgctgc aagtctagca attggcactc taaatggtga
    11641 acaagataca tagttaagtc ctacactata acagaactct actgaagatg gttctcctcc
    11701 atgctctcca catattccta gttttaattc tggtttaact tctctaccta atttagctcc
    11761 catcttaact aacttaccaa caccattttg gtctagtact tggaatgggt ctttttctaa
    11821 tatttcttca tctacatatt gacctaagaa tttaccagca tcatctcttg aatatccaaa
    11881 tgccatttga gttaagtcat tagtaccaaa actgaagaag tctgcttctt gtgctatttc
    11941 atcagcagtt aaacaagctc taggtatttc tatcatagta cctacagtat aaggaacttc
    12001 tataccttct tcttttatta tagcgtcaac agtttctact atcatttttc ttattatttt
    12061 taactcattt acttctccaa ctaatggaac cattatctct ggagaaactt taacacctgc
    12121 tttgattgct tctatagcac cttgtattat agctttagat tgcattacac atatttcagg
    12181 ataagttatt gcaagtctac aacctctgtg tccaagcatt gggttaaact catctaaatc
    12241 aactattctc ttcttaatat cacttacttt tattcccata gattttgaaa gttcttctat
    12301 agtctcatca tcatgaggca aaaattcatg tagtggtggg tctagtaatc ttatattaac
    12361 tggttttcca tccataactt taaataactc tacaaaatcc tctctttgca ttggtaatat
    12421 tttttcaaga gctttttctc tttgttctac tgtattagat aatatcattt ctctaactgc
    12481 tggcaatcta gcttcatcaa agaacatatg ctcagttcta catagaccta taccttctgc
    12541 accaaactct atagctgcac gagcatctct aggattatct gcattagttc taaccatcat
    12601 acatttgtgt ttgtctaccc agttcattaa tttttcaaaa tttccagtta aagatacttc
    12661 tgttttctta acatctccta ggtacacatt tcctgtagaa ccatctatag aaatgtattc
    12721 tccttctttt ataactacat catcaagtgc tcttatttct ttattaaact catcaacttt
    12781 tatttctcca catccagcaa cacaacattt acccatacct ctagcaacaa ctgctgcatg
    12841 tgatgtcatt ccacctctag ctgtaaggat tccttctgct ttaaccattc cttctatatc
    12901 ttctggagat gtctctagtc taactaaaac tactttttct cctttttcat ttgcttttac
    12961 tacatcatca gcattgaaat aaactttacc acttgctgca cctggtgatg ctggtaaacc
    13021 ttttgctata accttagctt cttttaatgc cttatcttca aatttgggat gtaataattg
    13081 gtctaattga ttaggctcaa ttctcatgat agcttctttt tcatcaatta ttccagcttc
    13141 aactaaatca accgctacat ttattgctgc tgctgctgtt ctcttaccat ttctagtttg
    13201 tagtatatat aatctttcat tttctatagt aaactctata tcttgcatat ctttataatg
    13261 accttcaagt atatgagtaa tttttacaaa ttcatcgaat acagctggca ttacatctgc
    13321 aagagttgat atattttgag gagttcttat acctgcaaca acgtcttcac cttgtgcatt
    13381 cattaaaaat tcaccaaata atttattttc tcctgtagct ggatttctag taaatgcaac
    13441 acctgtacca ctagtttctc ccatattacc aaataccatt gattgaatat ttactgctgt
    13501 tcctaagtta tgagctatat cattcaattt acggtatacg attgcacgag gattattcca
    13561 tgatctaaat acagcctcta ttgcaagcat taattgctct tttgggtctt gtgggaaatc
    13621 attttttatt tcttttttat aaattgcttt aaattctttt actatttctt ttaaatcatc
    13681 agttgtaagt tctgtatcaa ctgtgtatcc ctttgcttct tttactctgt ctaatacgtt
    13741 ttcaaattta tattttggaa cttccatagc aacatcagaa aacatttgta tgaatcttct
    13801 gtaactatca tatgcaaatc tttcattttg tgtagcctct gctaagccaa caacactatt
    13861 gtcattaaga cctaggttaa gaattgtatc catcattcct ggcatagaga atactgcacc
    13921 agaacgaact gatactaata atggattctt attgcatcct aatgttttat ttaaatcttt
    13981 ttctaaagta gctaaatgct cttctatttc ttttattatc tctgctctta tactttcatt
    14041 gttaacataa taatcattac acgcttctgt tgttatagtg aatccaggag ggacaggtaa
    14101 gcctatctta gtcatttctg ctaaattggc acctttacct cctagtaaag atttcatgtc
    14161 tttgcttcct tcaccaaaac tataaacata cttagtttcc attgttaaat cccccattaa
    14221 tctttttatt ttaatttata tcatttttat taatagacat tattaaattt ctatttcttt
    14281 gtatatcttt agaccatttt ctttcattat gtctaaaatt atacctgctg tttcttctat
    14341 tgccttattt gaaacgttta ttactgggca tccaattctt ttcattatct cttctgaata
    14401 atctaactcc tgaagtattc tttctaaatt agcgtagttt gctttactgg acaatcctaa
    14461 agcttttaat ctttgggttc ttatctcatt cagcttttca ggtgaattag taagacctat
    14521 tatcttttta gtatctatct caaacacttc tttaggaatt ggtatttctg gaactaatgg
    14581 aacattagct acttttatat ttttattagc taaatacata ctcaaaggag ttttagatgt
    14641 tcttgatatt cccactaaaa ttatgtctgc ttgaagaaca cctctagggt ctttcccatc
    14701 atcatatttc actgcaaatt ctatagcttc aactctctta aagtaagatt catctaattt
    14761 tctaattata ccagcttctc ttttaggttt tcttcctgtt ctcttgctta tttctcttag
    14821 tatatctgtc attaaatcta tacaactaag accttctctt tcgcaatatc tttcaacaat
    14881 actgcataat tcttcatcaa ctaatgtata tacaattaaa gcgttttcag cttttgcatt
    14941 ctccaaagtt tcaagtagta ctcctttatt aatcatgtat ggaaatcttt ttatttcata
    15001 agtttcattc acataaaact gagacataca tgcctttgca acttgttgtg cagtctcccc
    15061 tactgaatct gatactgcat atataattag gtttttcacc agcatacctc cttaattgtc
    15121 aactatttct aagaatagct tagttatgtt ggtttttgat attctcccaa caactctcat
    15181 atgatttttg tcttctccct tatattcaac aatcggaatc gaatctactt catgttctat
    15241 tatttttctt gaagctaata aaacacttgc tcctttagtt aaagtgacta catttggagt
    15301 tcttgtcatt atcataccta ttggcatttt atttatatca gaacctccta tagttgcttt
    15361 tagcaagtct tttctggaaa caactccaca taactcttca ttctcatcta taataactat
    15421 actccctaca tcagataaaa acatagtgac tattacatca tatatacttt catccttctt
    15481 agctagtaca ggcatgctca ttatatcctc tactgttttt tcttttacac ttttaccaat
    15541 taaatttatt tcactaactc ctgaataaaa atatccaact tttggccttg cgtctaatat
    15601 tcctgtcatt gttaatatag ctaaatcaga tctaagtgta gcacgagtca cattcaagct
    15661 ggaggcaata ctttcactcg ttattggttc attttcctta actatatcta taatttttag
    15721 ttgtctttca ttaagctgaa taataatcac cttctttcat tgtgctatac tttttatctt
    15781 ttcgcatctt atatagtata ttatataact atatatacca ttctgtaaac agatttttta
    15841 ttgaatacga tatatttttt actaagttct ctcgcttaaa gatatttatt atatattaat
    15901 gggggatatt tctttaatta atagaactat attagtatta cagttaatat tataatacat
    15961 aattggtcat tttaattcat tttatattaa aatttttaat aatattgttt caatgtgaat
    16021 ataaaattgc agaactaatt caaaataatt tttacgacta caaataacct ataatatact
    16081 tttaaagaaa gtatcctaac aagaatatat aaaaatttaa aagggttgat ttaaaaataa
    16141 ccatcaaccc tttttactaa tatctattta tattaccatc aaatcttacc caaatatttt
    16201 ttcttgcaat tgtcttaaat gatataaagt agtatctttt tctatctcta ccatatcgta
    16261 agttataact tcaccttttt taatatcttt tacaacttta gtctttttac ttatcaaacc
    16321 aataggtaca gcattcatag cctttgctac atcgtatttt tctatagtac catagcatgt
    16381 atatgctcca tagccatcca taaactcacc ttttttcaaa tctttcttag ctatagtcat
    16441 aacttctgct actagtccag ctttaggtac tattgtagcc ttatggtcta aaactgccat
    16501 agctgctgat aaaggagtct ctaaactaca taaatgatat ggtctatata atatgtaatt
    16561 aggtccatca cccatactta aatattttag ttcatcatta actgctttta atttatgggc
    16621 aactactacg aaaactccag gtgctatacc atttacaaaa tcaactacac cataattatc
    16681 taatactcca ccttcgcttt ttaatgcata tttcttaggt acttcattaa ctgtagcttc
    16741 aataccatga ccacctctta catcaggcac aaatccagtt gcattacaca ttaaagccat
    16801 ttctaccatt gttttagttc cttctttaaa agaagctatc atatgaggtg aagctccttt
    16861 tctttctgct tcttctctaa ctgtatcagg attacaatct aaatctaatt tattattttt
    16921 acctttacct atcactctaa cttcaaaccc cattgcatct gcaaaatcaa ataattccat
    16981 tactgcacct ggctcatctc ctgcagaacc tgtaaatatt actccagcat catctgctaa
    17041 ttttttaagt attggtccaa caacacaatc agtttcagca tttaacataa ctatatgttt
    17101 tttatttaaa atagagttta atgctatctc tgcaccaatt gcaatacccc cagttgcatc
    17161 tataactaca tctattgact tagttttaca agccacaaga aagtcattac atgtcaatac
    17221 ttttccttct tgtaatttta tttctgcatc cttttcattt gttacattat ctataatttc
    17281 ttcatctatt cctgcatcag taaaagcttt atgagccaac tccaaattta tatctacaac
    17341 aatagaagct tcaatacctt tcatatttgc catttggctt accattccat ttcccatatg
    17401 cccagctcct ataagagcta cttttatttt tttaccttcc tcttccaatt ttgcaagttt
    17461 tctgtttaaa tctaacatac caattcctcc tgttataata attttatttt caaattaaga
    17521 aatagttatt aaatctccaa tagttatatc tggatttcct atttccacat gtatttctcc
    17581 tggtaaattt acttcacatt ttccatcaaa tttgattgta caatgcccaa tttctctaag
    17641 agtcttctga gcaatatcac caactgaaac tatacaataa tctctattcc ctatagtaag
    17701 tgtatctcct atttcaacat cactttttaa tgttgctatt gaatgaatta cagatatatc
    17761 ctttaagtcg tcatcattaa tggtttcatc atatataata agacaattgc tttcatccat
    17821 taactcttta actaaactgc ctatcccact tatcttaact tcatatttca tgacattcct
    17881 cctgataatt tatatttata ttgagttaat taaaagaaac atttattttt tcttttataa
    17941 attttatagc ttgtacaata ctctcttcat ctttaaattt ttgctctgca ctatatatat
    18001 ctaatccttc tatttcttca atctctttaa atttagccat tactgtacgc ccttttaaaa
    18061 tttctccaaa ctttataaac ccatctgaat ctgaagatat aatggctata cttcctttag
    18121 aaaaatagct tttctttttg cctgtagaca ctatatcacc ctttttttta gctctattta
    18181 aagttttaaa atacaattta ttctgcttca ttataaatat agtatttaaa atataagcaa
    18241 caaataaaat tactaataat ttcatattta tacaccttcc aaaaatcaca ttatttttaa
    18301 atctgtataa caactataaa tgctgtacaa tagttatata aggtttttaa gtaacacctt
    18361 tttaaattct aaaaaagttc tacatttact tatcttattc gttaatttta catcttggcc
    18421 tatctttaaa acttcttcat aaatttttac taataagtct gagtctatct tattctcaca
    18481 tggaaacatt gtcataagaa ttatttttat acttctattt gcatattcta ttggttcttc
    18541 taaaattcca actgccatta aaaacttatc tgatttttca tttatagaat gaggaaataa
    18601 taaaccttta tcaaatactg tgggagattt ttcttctcta ttgactatat tttttctaaa
    18661 cttctcatca atacatccca aatccataag atggtccatc attttttcta atgaggcaag
    18721 tatagtcttt tcatttaata tcataaattt atcttcatct aacaaattat ttattagtaa
    18781 atttgtagaa ttagtagtgt caatattccc ttctcttagg cacaatactg tttttaattg
    18841 ttctcttagt ttgttttcat caaatattgt atttattttt aatactattg tatttatatt
    18901 tgatgtgtct aaatcaatag tcgtaaaaac tatatcgtat tcatctaagt taatatttct
    18961 caaattaaaa cttgaaagag tatcaatttt aggattattt cctatcagtt tttctactct
    19021 tattttcaat agttttgctg ttcctagacc tgtactacat ataattgcta ttttttttat
    19081 tgaatagaat ctactgctat ttctttctaa atatccactg aaatggattg ctatatatcc
    19141 aatctcatct tctgaaactt ctaagttata ctttttctct attacttttc ctgctatttg
    19201 tgctaattta aatggaagta tacaattttg cttaatctct tctaacaaaa cattttttaa
    19261 ctttatatta aataatagcc tgtttaaagt aaaactaaga tgatatccta gacctactct
    19321 taattcatcc tcatttataa aaatacccat atcgacatat atttgattaa atatattttc
    19381 catcaactca tcaatgtttt tactaattct actactattt aaagcacatt ctgatgctg
//
LOCUS       DAELLV010000049        19433 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.049, whole genome shotgun sequence.
ACCESSION   DAELLV010000049 DAELLV010000000
VERSION     DAELLV010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19433
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.049"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            29..880
                     /locus_tag="KPD58_001466"
     CDS             29..880
                     /locus_tag="KPD58_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HBG0265244.1"
                     /translation="MKNIISAKELISKLEKNDNLVVIDCRFDLINRTYGIDSYKKGHI
                     KGSFILDIDKDLSSPTKEHGGKNPLQDPLILKEKLEKMGVDNDTTIVTYDDGDLNGAC
                     RLFFQLKHLGLKNVYVLDGGITSFVKEGGELEEKVNIPSCTGKEIRPNINNDLVVPME
                     YVKSKLYKKDTVIIDCRANERYQGLVEPAYSKAGHIPSAKNYFCKDLIKSDFENGSLK
                     DIEFLKKFFKDLNNYDEVILSCGSGISACVNSLALRELDIPHKIYIGSFSDWISYDDN
                     EIKTGQE"
     gene            complement(1013..2626)
                     /gene="ggt"
                     /locus_tag="KPD58_001467"
     CDS             complement(1013..2626)
                     /gene="ggt"
                     /locus_tag="KPD58_001467"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HBG0265245.1"
                     /translation="MKFNAYQHKYSSIRNVVYAKNGAVATSTPLASQAGLEILKKGGN
                     AVDAAVATAATLAVVEPTSNGIGGDAYALVWIEEEKRLYGLNSSGFAPENMELKNYNN
                     MKEMPKYGFGAVTVPGIPAAWSELNKKYGKLSLMECLSPAINYAREGYVVSPNVAKVW
                     KKSYELYEKELIGEEFKPWFDTFSKDGKAPEAGDIFICEEQASTLEEIANTQAESFYR
                     GRLADKIDEYSKKFNGAIRKSDLECFYPTWVEPISTEYKGYKIFEIPPNGHGITVLMA
                     LNILKELELEGNIENVEDIHKIIESLKLAFADSKTYVTDIEHMKVKIQELLSQEYAKK
                     RSLLIDNKEALYPTAGEPYCGGTVYLCTADKDGNMVSYIQSNYINFGSGIVIPRTGIA
                     LHSRGNNFNLDPKHHNVVKPFKKPYHTIIPGFLGKEDKAIGPFGVMGAFMQPQGHIQV
                     LTNMIDFGLNPQEALDAPRWQWIKGKEIEVEPEMPKHIIDSLIEKGHEIKVIHDTVDM
                     GRGQIIFKTEQESYICGTESRCDGHIAVY"
     gene            complement(2851..4787)
                     /locus_tag="KPD58_001468"
                     /pseudo
     CDS             complement(2851..4787)
                     /locus_tag="KPD58_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(4774..5541)
                     /locus_tag="KPD58_001469"
     CDS             complement(4774..5541)
                     /locus_tag="KPD58_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003722224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0265246.1"
                     /translation="MEEILSVENIKKEYGRKGSKHEALRGITFKVYKGEFVGIMGSSG
                     AGKSTLLNIISTIDLPSSGDIYINGKNTIKMKQNELADFRRDNLGFVFQDSNLLDTLT
                     IKENIMLPLSLKNERVSVIENRIKEISKELNIESILDKYPGEVSGGQKQRGAVCRAIA
                     TKPSLVLADEPTGALDSKSARDLLNCLLKLNKDSNKTILMVTHDAISASFCNRILFIK
                     DGIIFTEIVKGESNREFYNKIVNTVSLIGGVNKNDFI"
     gene            5829..6503
                     /locus_tag="KPD58_001470"
     CDS             5829..6503
                     /locus_tag="KPD58_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0265247.1"
                     /translation="MFKIMVVEDDVSLKNIIAKCLTKWGHDVHQIENLENIIEEFKNY
                     NPELVLLDINLPFYDGFHWCNEIRKISKVPIIFISSRNSNMDVIMGVNLGADDYIQKP
                     FSVDVLVAKVNALLRRTYNFVDNNSNQIIHNGVTLDLSTATINYEDNTIELTKNEIKI
                     LHELMKYKGQIVSRNKLMKKLWDNDWFVDDNTLTVNVNRIRSKLNEIGLEDFIETKRG
                     LGYIIS"
     gene            6527..7531
                     /locus_tag="KPD58_001471"
     CDS             6527..7531
                     /locus_tag="KPD58_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0265248.1"
                     /translation="MSFKDFLKDKLGFLVYNFTFLIFTVSVIIFSPVDVFLTDTILYI
                     LVVNVVFLFLYLFISYIKKNKFLDKIKNDTFKINMNDIELARCKNEEKIYLNIIKNHQ
                     YQCNNIINNNIEKFEENKEILNMWVHDVKIPIAIIKLILEQNENLIDEKISDDIDSEI
                     FRIENSVEKILYLSRLEDFHKDFLIQEVNIEKIIRNIIKKYSKYFISKKIILDLNNLN
                     FKVLSDEKWLYFIFEQLLSNSLKYSSLGDNISIYCKRTKNYLQLRVEDTGCGIKKEDL
                     NRIFNKGFTGNNGRNNSKSTGLGLYLVKELCDKLGHKIDVDSEYNQYTKFTITFKCDL
                     "
     gene            7643..8872
                     /locus_tag="KPD58_001472"
     CDS             7643..8872
                     /locus_tag="KPD58_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBG0265249.1"
                     /translation="MSFLKKFKNKRFRNNSFKSNNFRNSSFGSSGFKSKNKGFKNNPF
                     KNKRFKIYAGIAVIILLILGVLAYVDSKNTELQANENFIETYTIPENEKIFINGMVVP
                     KQTKDFNISGDYELSDVNVTNGQKVNQGDLLFTAKNPTIIAEIDSLKSQLSQYKKQKI
                     SLSDIAENKDAIASINAQITALNSQIASLDKKAYDRVTAPFDGTVYLNDQTGNPDQPV
                     SFMTIQGLEFYMKGQASEQDLPKMKIDQMVNILVFSTDQKLTGRISFISDKPSTPNTE
                     MGAQQNTLSYYDINIAFDNQEGLVNGFHLQASLEVANSSFKIPASCVLKDNKHSYVFK
                     DLDGILKKQIVDVASQNDDFAVVRGGLEQGDIIIKHPTKEMKEGDPVQGDGVTAGSNN
                     GDTTPNKKVEEVTMDVN"
     gene            8880..9554
                     /locus_tag="KPD58_001473"
     CDS             8880..9554
                     /locus_tag="KPD58_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0265250.1"
                     /translation="MLIKLENIQKYYKVGKDELHVLKSLNLEIESGEFVMIMGKSGSG
                     KTTLLNILGFLDVFDEGRYIFDGTDVTNLSENERSVFRNINIGFVFQQFNLIETLNVY
                     QNVELPLIYNKALKKSNREEIVKDKLSSVGLLDKLKQKPLQLSGGQQQRVAIARCLAN
                     DPQIIFADEPTGALDSETSREIMELLTRLNKQGKTIIMVTHDQDLTKYATKVIRLKDG
                     VFTSEV"
     gene            9557..10789
                     /locus_tag="KPD58_001474"
     CDS             9557..10789
                     /locus_tag="KPD58_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0265251.1"
                     /translation="MSLLKNSLANLKGHKLRVFVALLWIIIGITSVILVSSIGNGFQK
                     EIKKSVNNVNPNKTTISFESADNTGLTDDMSIFLKPFNAKDLEELSFVEGVERIAPSR
                     DGFNLDSVYSSQASFDKKTTYVDVGPVKKDSKINLICGRDFSLDDEKRKVILLTLQST
                     SEIFENPEDALGHGININGTIFEIIGVLDDSQQNQAGGFFGGYQDMQFTTSLVPKKAF
                     DTLMSQNSYSNEIYQLDLVSSKGYNVNEVANNVIAKLYEMHPGINGSYTTPDPTEQTA
                     YLESINSNVNKYVSIITVVAMFVGGIGVMNIMYVSVMERQREIGIRRAIGAKPRSILF
                     QFLVEAVFITVCGGILGTIVGFAATNYVSKYIGFEAIPSLNSLFYAIVATILTGVVFG
                     LIPAFKASKLDPIKAIYK"
     gene            complement(11075..12340)
                     /locus_tag="KPD58_001475"
     CDS             complement(11075..12340)
                     /locus_tag="KPD58_001475"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBG0265252.1"
                     /translation="MILIKNGRVIDPKSQRDENIDLIIKENKIYKIGKFDESDEYEKI
                     IDASGNVVAPGLVDVHVHFRDPGFTYKEDIESGAKSAARGGFTTVICMANTNPIVDNE
                     DTFNYVKEKSKNACINVLQAAAITKGFEGKELVDMEALKKAGVPGFTDDGLPLMDSNL
                     IMEAMIKAKELDVPLSFHEEDPSLVGNPGVNAGKVASELGLKGASSVAEDVMVARDCM
                     LALKTGAKVDIQHISSGVSVDMVRFAKYLGANVVAEASPHHFTLTEEDVLEFGTNAKM
                     NPPLRSKWDRDKIIEGLNDGTIEIIATDHAPHSKEEKDREFIKAPSGIIGLETSLALG
                     ITNLVHKNHLSMMQLIEKMSINPAKLYNLNIGFIEEGAVADIVIFNPEEEWTVESFVS
                     KADNSPFKGKSLYGKVNYTICNGEIVYNA"
     gene            complement(12393..13541)
                     /locus_tag="KPD58_001476"
     CDS             complement(12393..13541)
                     /locus_tag="KPD58_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HBG0265253.1"
                     /translation="MKSVSVRSCKTYNYEEVKLSIEKNLEDIGGIDKFVKKGSKVLIK
                     PNLLMKKKPEEATTTHPMVVKVVCEKLLELNCDITIADSPGGPYNQSSLKSIYKASGI
                     EEVANELGIKLNYDVSDVKIHSEKAHVLKYMDIIKPIVDSDYIINLCKLKTHIMATFT
                     GGTKNLYGCIAGLKKAEIHYRFPTEELFCENVLLDICDYIKPTLTIMDGIVGMEGDGP
                     SAGTTREIGVLLASQNPYAIDVVACKIISLLPNRVATIRGAIKRGYIQEDFSDIEILG
                     EDIKDLIIKNYKIPNVSKDLRLFSVKLPKFLNEPISKLITPKPVVRFQDCIKCGKCKE
                     ACPASTIEIGTKGAIIDLSKCIRCYCCHELCPKKAIDVKRNFVFKYVK"
     gene            complement(13641..14186)
                     /locus_tag="KPD58_001477"
     CDS             complement(13641..14186)
                     /locus_tag="KPD58_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HBG0265254.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEMVWADE
                     HRIGVAQGVDAEIIEGLRANFTGECTEVGMYLAMSRQADREGYPEVAEAYKRIAFEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKRAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRHFGK"
     gene            complement(14409..15353)
                     /locus_tag="KPD58_001478"
     CDS             complement(14409..15353)
                     /locus_tag="KPD58_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBG0265255.1"
                     /translation="MQKNKKEGALLKKYFGEIGLIFIAIIWGSGFVATQFALDGGLTP
                     LQIITLRFFLAAIIMNLLFFKQIRANMGKKLLKAGGILGIFLFLAFTVQTIGLMYTTP
                     SKNAFITAANVVIVPFIGFILYRRKLDKIGIISSLVALIGIGILSLEADFSINFGDFL
                     TLICSFGFAFHIFFTSEFAKDNNPMALTAIQFTVAFLMSVVVQTFAGQLKMEAELSGY
                     MGTMYLAVFSTTIGFLFQTICQKRVDGTRTAIILSTEAVFGTIFSIIILKELITAKLV
                     IGSILIFVAIITAETKLSFLKSKKVKLKDSEESSLESI"
     gene            15739..16620
                     /locus_tag="KPD58_001479"
     CDS             15739..16620
                     /locus_tag="KPD58_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HBG0265256.1"
                     /translation="MVKKRKKKTVALTILVVIVIGIFSMAWADMSRKKEMKETQERLK
                     AERLEKERIEKEKNEPIIGAKKEAVKEYGYDAKIVWDKLNKYDYSNNGEKIVFLTFDD
                     GPSTTNTPQVLDILKRHGVRGTFFIKGDSLERKGANEILKRTFDEGNAIAHHSYTHDY
                     KKLYPNRSLNLDTFVNELNKTDEAMKKVLGKNFSSNVVRCPGGYMSWKNMEPLGNYLK
                     EKNMASIDWNALNADAEGKKKNAQELFEHAKKSSEGKEMVVLLMHDTYGKQETVNALD
                     QIITYFKDNGYQFKILI"
     gene            complement(16681..17889)
                     /locus_tag="KPD58_001480"
     CDS             complement(16681..17889)
                     /locus_tag="KPD58_001480"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBG0265257.1"
                     /translation="MKSIDEQMRIIMKGVDDLIDEKELREKLIKSEKEGKPMIVKLGL
                     DPSAPDIHLGHTVVLRKMKQLQDLGHQIVIIIGDFTGKIGDPTGKSKARKALTTEQVL
                     ANAKTYEEQIFKVLDKEKTIVRFNSEWLAKLNFEDVIKLAATITVARMLEREDFKKRY
                     EGQMPISVHEFFYPLMQAYDSIALEADIELGGTDQRFNLLMGRSLQREFGMESQIVIM
                     MPLIEGLDGKEKMSKSLGNYIGIDEEAGIMYQKSMEIPDELIIKYYNLVTDVHPDEVN
                     KIESQLKEGSVNPRDIKMDLAREIVTLYHGEESAKEAEERFKSVFQKGQIPEDIQTIQ
                     VKEDGFDLIEVLVSNEIVKSKSEVRRLASQGGVKVNGEKVEDLSTIVKESELVVQIGK
                     KKFVKIELVK"
     misc_binding    complement(17962..18214)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(18366..19355)
                     /locus_tag="KPD58_001481"
     CDS             complement(18366..19355)
                     /locus_tag="KPD58_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HBG0265258.1"
                     /translation="MIYGGSVLNTTKKLTEAALLSSLFIVVTIIAVSTGFGYAIYLDF
                     IVPVFFCIICLKCDLKYTILSGITSLLIISLVLGNLGTAIWASQSVLLGIMCGYLINK
                     PTMVMDDLVYGSVFGVIVMVFIDIYASTLIGYSFMKEFQGYSKWIYFNGYTNFIYYLM
                     IALFPFGMVFCIYLLSLILGNKLHILDSNSLKKFLIFKNFRNLNQFLCCSKKAFYICV
                     SYLAIFEVLKLLNVKVDSVYLKTIFISITYISVYFIIRDSCMSLQNFIIAKFRKLLYA
                     RILFLIIILLLFFIFDVTVIGLIIINAFLNKKINIRLSQSNIVNKQINLLIEK"
BASE COUNT         6822 a         3046 c         2379 g         7186 t
ORIGIN      
        1 gataatataa acacaggagg tacattcttt gaaaaacatt atttcagcaa aagaacttat
       61 atcaaaacta gaaaaaaatg ataatctagt tgtaatagat tgtagatttg atcttatcaa
      121 tagaacttat ggtattgatt cttataaaaa aggacacatc aaaggcagtt ttatcttaga
      181 tatagacaag gatttatcat ctcctacaaa agaacatggt ggtaaaaatc ctttacaaga
      241 ccctttaata ctaaaagaaa aattagaaaa aatgggtgtt gataacgaca ctacgattgt
      301 aacttatgat gatggagatt taaatggagc ttgcagatta tttttccaat taaaacattt
      361 ggggcttaaa aatgtctatg tactagatgg gggaataaca tctttcgtaa aggaaggtgg
      421 agaattagaa gagaaagtga atataccaag ttgtacaggt aaggaaatta gaccaaatat
      481 aaacaatgat ttagttgttc caatggaata tgttaagtct aaattataca aaaaagatac
      541 tgtaataata gattgtcgtg ctaatgagag ataccagggt ttagttgaac ctgcttactc
      601 aaaagctggt catataccaa gtgcaaaaaa ttatttttgt aaggatttaa taaaaagtga
      661 ctttgaaaat ggctcactta aagatataga atttttaaaa aaattcttta aagatttaaa
      721 taattatgat gaagtaattt tatcttgtgg ttcagggata tctgcttgtg ttaacagtct
      781 tgctcttaga gaactggata tacctcacaa gatttatatt ggtagcttta gcgactggat
      841 tagttatgat gacaatgaaa tcaaaactgg tcaagaataa ggctcataat ctatataaaa
      901 tcagcatatg atagcttaaa ttttgtaaag gcgtcagtca ttgtagactt tcgcctttac
      961 ttattatttc atatactctt taaatttatt atatatattt tagttgataa tattaataaa
     1021 ctgcaatatg accatcacat cttgattccg tcccacaaat ataactttcc tgttctgttt
     1081 taaatattat ttgacctcgt cccatgtcga cagtatcatg aattactttt atttcatgac
     1141 ccttttcaat taatgaatct attatatgct ttggcatctc tggttcaact tctatttctt
     1201 ttccttttat ccactgccat cttggagcat ctagcgcctc ttgtggattt aatccaaaat
     1261 caatcatatt tgttaacacc tgaatatgtc cttgtggttg cataaaagct cccattacac
     1321 caaatggacc tattgcttta tcttctttac ctaaaaatcc aggtattata gtatgataag
     1381 gttttttaaa tggttttact acattgtggt gtttagggtc taaattaaaa ttatttcctc
     1441 tactatgtaa agcaatccca gttcttggta tcacaattcc tgaaccaaaa tttatgtaat
     1501 tgctctgtat atatgaaacc atatttccat ccttatctgc agtacaaagg tatactgtac
     1561 caccacaata tggctctcct gcagttggat acaaagcctc tttattgtca attaataaac
     1621 ttcttttttt ggcatactct tggcttagta attcctgaat ctttaccttc atatgctcta
     1681 tatcagttac atatgtttta gaatctgcaa aagctaattt taaactctct ataattttat
     1741 gtatgtcttc tacattttct atatttcctt ctaattccaa ttcttttaat atgttaagtg
     1801 ccattaaaac tgttattcca tgaccatttg gtgggatttc aaatatttta tatcccttat
     1861 attctgtact tataggttct acccatgttg gataaaaaca ctctaagtca ctctttctta
     1921 tagccccatt aaatttttta gaatattcat ctattttatc agcaagtctt cctctataaa
     1981 agctttcagc ctgagtattt gctatctctt ctaatgtact tgcctgttct tcacatataa
     2041 atatatctcc agcctctggg gctttaccat ctttagaaaa tgtatcaaac catggtttga
     2101 actcttctcc aataagttct ttttcataga gttcatatga ttttttccat accttagcta
     2161 cattaggaga tactacgtat ccctctcttg catagtttat cgctggagat aagcattcca
     2221 ttaagctaag tttaccatat tttttattta attctgacca agctgctggt attccaggta
     2281 cagttactgc tccaaaccca tattttggca tttctttcat attattataa ttttttaatt
     2341 ccatattttc tggagcaaag ccactagaat ttaaaccata caatcgtttt tcttcctcta
     2401 tccaaacaag ggcatatgca tctcctccaa tcccattgct tgtaggttct actactgcta
     2461 atgtggcggc tgttgcaact gctgcatcaa cagcatttcc accctttttt agtatttcaa
     2521 gtccagcttg agatgctaat ggtgttgatg ttgcaactgc tccattttta gcatacacga
     2581 catttcttat tgatgagtat ttatgttggt aggcattaaa cttcataaat ttcagctcct
     2641 cgtcattttt tatttgatat tctgcttaga tttattatat aacatcaagt aatgtaactg
     2701 atacaaaatg atataaaata tagacttttt cctaacttta attttatttt taaataaata
     2761 tcttaaactc aattctagta ttttctatct gcacttaaat aagttatttt attcaaaaca
     2821 tatatctaat tcttttaact taaaaataat ttattctttt atcatgttta gagcatattt
     2881 taaagttaaa aaataatata taaaatatcc tataaaataa atgactagag ttatgataat
     2941 tggtacaagt aatgaccttg gtataaactt ttgtgctata gacatagcaa ataaattatg
     3001 aactgtacct acaactaatg gcattaaaaa tataactttt aactgttttg atattatttt
     3061 ctcaatatct ttattatttg ctccaagttt tattaacata atatatcttt ccttatcatc
     3121 atatatattt gataattgtt taaataagat tatactacct gtacataaaa gaaatactac
     3181 agacaaaaat atgcctataa acagcattag accacttaat tttatcaagt tattataact
     3241 ttcaatactt gatgtaaaat taaaaggata agtaaatttc atattttcac ttactatatt
     3301 ttttaatttt aaagtcaaat ccagttcttt ttttcatttt ttatatcaat taatcttatt
     3361 aatctagaat ttccatgtaa ttttaactga ttaaatacgt catcttttac tacaattaaa
     3421 tcaaacgtag cttgtgaatt tatttctggc tccaagatgc ttttttgtac tttaaacaga
     3481 tttatatcat actcatttgc atctttaata tgcaagttta ttttttcaat ctcaaagtct
     3541 ttaaaaaata tttttttgta tgaatcagaa acatagtaca cagattcctt tgaagttaat
     3601 tcttcataat tgttattctg atattgtctt aattttttaa aatcactttc tctaataata
     3661 tctacagacg ttctatcttc tatataacta tttccatttt tataatacat ttcaatattt
     3721 atatctttgt ctataagttc tattgtttta tcaaagatta tcttattatc ttttttatat
     3781 ttatataata atttgtcaat ttcgttattt acataagaat ttcctaaatt tatggtataa
     3841 ctgtatttat aattttcact tatgttccta tctatatcgt aataaagtga tatggtaaac
     3901 cctagcatag ttacacttgt agctattaat attgctatta cagccaatac actactattg
     3961 ctctttaatt taaatccaag ttcagaaaat gcaattaaat tctgtccctt ataataaaaa
     4021 ctttttctct tcttaacagc atcaataatc atatacatag ttgatgaaaa aaataaaaaa
     4081 gttccaggta ttattgtaac taatataaaa actgaaagca taatattttc agcaataaaa
     4141 taactaattg acattaaata agaaatagct attaataaaa caccaagtac acattttaac
     4201 tttataaatt ttttaaactt atacttttct ggatatttat taaataactc tataattctc
     4261 ttatttctga taactatagt atttcttatt ccaataacta taaatattat taaaaataca
     4321 gctaatgtct gaactaaggc ctttatactt aagttcatat ttactactat catttcattc
     4381 attaatttta caagcatcat agtcataagc tttgaaaata acagtcctaa aaatatacct
     4441 attagtattg caactatacc catcaaaaaa gtttcatata aaaatatttt tcctatctga
     4501 tttttcctca ttccaagtaa actataagtt cctacttctc gcctacgtct attgaaaaaa
     4561 aatgagtttg aataatatat aaataaaaat acaaatacca ctattacaat tgaacttgta
     4621 ttaatactca tcctggtttt ctctccaagt gaactcaacg cttcattata ctctatagat
     4681 ttaaaactaa aaaatacaaa tacactaaat acaattgaaa taaagtacat taaataatta
     4741 aaaaaactat tctttacatt ttgcattgca attctaaata aagtcatttt tattaactcc
     4801 tccaattaag gaaactgtat ttactatttt attatagaac tctctgttac tttctccctt
     4861 tacaatttct gtgaatatta ttccatcctt tataaagagt attctattgc aaaaactcgc
     4921 acttatagca tcatgagtta ccatcaatat tgttttattg ctgtctttgt ttaattttaa
     4981 tagacaattc aacaaatccc tagcagattt agaatctaat gctcctgtag gctcatctgc
     5041 aagtactaat gatggttttg ttgctatagc tctacataca gctcctcttt gcttttgtcc
     5101 accagaaact tcgccagggt atttatctaa tatagattct atatttaatt cttttgaaat
     5161 ctcttttatt ctattttcta ttactgatac tctttcattt tttaaagaaa gaggcaacat
     5221 tatattttct tttattgtaa gtgtatccaa aagattagaa tcttgaaata caaagcctag
     5281 attatcacgt ctaaaatctg ccaattcatt ttgcttcatt ttaatagtat tctttccatt
     5341 tatataaata tctcctgatg aaggtaaatc tattgtagaa ataatattta aaagtgttga
     5401 tttaccagca ccggaagacc ccataatacc aacaaattct cctttataaa ccttgaaagt
     5461 tattcctctt agagcttcat gttttgaacc ttttcttcca tactcctttt ttatattttc
     5521 aacagataat atttcttcca tagttaataa tcacctttca tcacaattag ttatttatca
     5581 attctcacaa atatattata tatattaaaa tggcttttaa acatttaatt atattaattt
     5641 aaggttacat tttcgtaagt attaattata tttcttcaaa tttattgcaa ttatgtttaa
     5701 aatataattt atcaagtaaa atatagttat attaatataa ttattttagg taataaggta
     5761 gaaatatttt aatttatcaa gtagaatata gttatgttaa tataactatt ttgaattagg
     5821 aggtaagaat gtttaaaata atggtagttg aagatgatgt ctcattgaaa aatatcatag
     5881 caaagtgtct tactaaatgg ggacatgatg ttcatcaaat agaaaatctt gaaaatataa
     5941 tagaagagtt caaaaactat aacccagaat tagttttatt ggacataaac cttccatttt
     6001 atgatggttt tcattggtgt aatgaaataa gaaaaatttc aaaagttcct ataattttta
     6061 tttcttcaag aaattctaat atggatgtaa taatgggtgt aaatttaggt gcagatgatt
     6121 atatacaaaa acctttttct gtagatgtct tagtagctaa agttaatgcc cttttaagaa
     6181 gaacttataa ttttgttgat aataactcaa atcagattat tcacaatggt gttactttag
     6241 atttatcaac tgcaacaata aactatgaag ataatactat tgagctaaca aaaaatgaaa
     6301 tcaaaattct ccatgagctt atgaaatata aggggcagat agtaagtagg aataaactca
     6361 tgaaaaaatt atgggataat gattggtttg tagatgataa tacattaact gtcaatgtaa
     6421 atagaatacg ctcaaaatta aatgaaattg gtttagaaga ttttatagaa acaaaaagag
     6481 gattgggtta tataatcagt taaaacaatt ctaagtaggt gaatttatga gctttaaaga
     6541 ttttctcaag gataaactag gttttttagt atacaatttt actttcttaa tttttacagt
     6601 atcagttatt atattttctc ctgtagatgt ttttctgact gatactattt tatatatatt
     6661 agttgtaaat gttgtgtttt tatttttata tctatttatt agctacatca agaagaacaa
     6721 atttttagat aagataaaaa atgatacatt caaaattaat atgaatgata tagaattagc
     6781 tagatgtaaa aatgaagaaa aaatatactt gaacattatt aaaaatcatc aatatcaatg
     6841 taataacata ataaataata atatcgagaa atttgaagaa aacaaagaga ttttaaatat
     6901 gtgggtacat gatgtaaaga tacctatagc tataataaaa ctcattcttg aacaaaatga
     6961 aaatttaatt gatgaaaaaa tttcagatga catagatagt gaaatattta gaattgaaaa
     7021 ctccgttgaa aaaattttat atttatcaag actcgaagat tttcataagg attttttaat
     7081 tcaagaggta aatattgaaa aaatcattag aaatattata aaaaaatact caaaatattt
     7141 tatttcaaaa aaaattattt tagacttaaa taatttgaat tttaaggttt tatctgatga
     7201 aaaatggcta tacttcatat tcgagcaatt attgagtaat tccctaaaat actcctcatt
     7261 aggagataat atatctatct attgcaaaag aaccaaaaat tatttacaat taagagtgga
     7321 agatactggt tgtggtataa aaaaggaaga tttaaatcga atattcaata agggatttac
     7381 aggtaataat ggaagaaaca attctaaatc tacaggtctt gggctttatt tagtaaaaga
     7441 actttgtgat aagcttggtc ataaaattga tgtagattca gagtacaacc aatatacaaa
     7501 atttactatt acattcaaat gtgatttata gtatcttatt attgatagtt tattttcatt
     7561 aacttatatt caacttctta tagtaattct atatgtacat cccagtatat aattaagtag
     7621 tttttataaa aaggaggaag acatgtcttt tctaaaaaaa tttaaaaata aaagatttag
     7681 aaataacagt tttaaaagta acaactttag aaatagcagt tttggaagta gtggcttcaa
     7741 gtctaaaaat aaaggtttta aaaacaatcc ctttaaaaat aagagattca aaatatatgc
     7801 aggaatagca gtaatcatac ttttaattct aggagttctt gcttatgttg attcgaaaaa
     7861 cactgaatta caagctaatg aaaattttat cgaaacatat actattcctg aaaatgaaaa
     7921 aatatttata aatgggatgg ttgttcctaa gcaaactaag gacttcaata tatctggaga
     7981 ttatgagtta agtgatgtta atgttacaaa tggtcaaaaa gtaaatcaag gagaccttct
     8041 ttttactgct aaaaatccaa ctataatagc agaaatagat agtttaaaat cacaacttag
     8101 tcaatacaaa aaacaaaaaa tatcactttc tgatattgct gaaaataaag atgctattgc
     8161 ttctataaat gcacagataa ctgctttaaa ctctcagata gcttctctgg ataaaaaagc
     8221 ttatgacaga gttactgctc cttttgatgg cacagtatac ttgaatgacc aaactggaaa
     8281 tccagaccaa ccagtttcat ttatgactat tcaaggtcta gagttttata tgaaaggaca
     8341 agcaagtgaa caagatttac ctaaaatgaa gattgaccaa atggtcaata tactagtttt
     8401 ttcaactgac caaaagctta ctggtagaat aagttttata tctgataaac catctactcc
     8461 aaatactgaa atgggagctc agcagaatac actatcttat tatgatataa acatagcatt
     8521 tgataatcaa gaaggattag taaatggatt ccatcttcaa gcatcacttg aagtagctaa
     8581 ttcaagcttt aaaataccag catcttgtgt acttaaagac aacaaacact catatgtatt
     8641 taaggattta gatggtatat tgaaaaagca aattgtagat gtagcaagtc aaaatgatga
     8701 ttttgctgta gttagaggcg gtcttgaaca aggcgatata ataataaaac accctactaa
     8761 agaaatgaaa gaaggagacc cagttcaagg cgatggcgta actgctggtt ctaataatgg
     8821 agatactact ccaaacaaaa aagtagaaga agttactatg gatgtgaatt aatttacata
     8881 tgttaattaa attagaaaat attcaaaaat attacaaagt agggaaagat gaattacatg
     8941 ttttaaaatc cttaaattta gaaattgaat ctggagaatt tgtcatgata atgggtaagt
     9001 ctggtagtgg taaaactact ttacttaata tcttaggttt tctagatgta tttgatgaag
     9061 gaagatatat atttgatgga acagatgtaa ctaatttgag tgaaaatgaa cgttctgttt
     9121 ttagaaatat taatattgga tttgttttcc aacaatttaa tttaatagaa actctaaatg
     9181 tatatcaaaa tgtagagttg cctctaattt acaataaagc actaaaaaaa tctaatagag
     9241 aagaaattgt taaagataaa ttaagttctg ttgggctttt ggacaagctt aagcagaaac
     9301 cacttcaatt atctggtggt caacaacagc gtgtagccat tgcacgttgc ctagcaaatg
     9361 accctcagat aatctttgct gatgaaccga ctggggcatt agatagtgaa actagcagag
     9421 aaattatgga gcttttaaca aggctcaaca aacaaggtaa gactataata atggttacac
     9481 atgatcaaga tttaactaag tatgcaacta aggttatacg acttaaagat ggtgtgttta
     9541 cttcggaggt gtaatcatga gtttattaaa aaattctttg gccaatttaa aaggtcacaa
     9601 gcttcgtgta tttgtagctt tactctggat tataatagga ataacttcag taatacttgt
     9661 tagttctata ggtaatggat ttcaaaaaga aattaagaaa tctgtaaaca atgtaaaccc
     9721 taataaaact actatttcgt ttgaatcagc cgataacaca ggtcttactg atgatatgag
     9781 tattttttta aaacccttta atgcaaaaga tttagaagaa ttatcatttg ttgaaggagt
     9841 agaaagaata gctccttcaa gagatgggtt taaccttgat tcagtttact cttcacaagc
     9901 atcttttgat aaaaaaacta cgtatgttga tgttggacca gtaaaaaaag attctaaaat
     9961 aaatttaatt tgtggtagag atttttcttt agatgatgaa aaaagaaaag taattttact
    10021 tacattacaa agtacaagtg aaatttttga aaatccagaa gatgccttag gtcatggtat
    10081 caacataaat ggaactatat ttgaaataat aggggtatta gatgattctc agcaaaacca
    10141 agctggagga ttctttggag gttaccaaga tatgcaattt actacttccc tagtacctaa
    10201 aaaggctttt gacactttaa tgagtcaaaa ttcttattca aatgaaattt accagttaga
    10261 cttagtttct tctaaaggct ataatgtaaa tgaagttgca aacaatgtta tagctaaatt
    10321 atatgagatg caccctggta tcaatggttc ttatactaca ccagacccaa ctgaacagac
    10381 agcttatctt gaaagcatta attccaatgt aaacaaatat gtatctataa taactgtagt
    10441 tgcaatgttt gttggtggta ttggtgtaat gaatataatg tatgtatctg ttatggaaag
    10501 acaaagagaa attggtataa gacgtgctat tggagcaaaa cctaggtcta tattatttca
    10561 atttttagtt gaagctgttt ttataacagt atgtggtgga attttaggaa ctatagttgg
    10621 atttgctgct actaactatg tttctaaata tataggtttt gaagctatac ctagcttaaa
    10681 tagcttattt tatgcaattg tagctactat attgactgga gttgtatttg gtctgattcc
    10741 agcctttaaa gcctctaaac tagaccctat aaaggcaatt tataagtaga aattgtataa
    10801 agattttcga tttttagatt agtttatata aaacatactt aaatatataa cgcactatta
    10861 tatatgatag attaggaact taatctttcc aaatataata gtgcgttctt tcataaaaag
    10921 gattctcttc cttactatat ctaaattacc aagatgttat gttctaattg ttcaaactaa
    10981 tacttccaat tttgattaat caatctcatt ctcctaatat acaatctttt actttcaatc
    11041 attgtacact ccgccttaaa aaaataatct taaattatgc gttatataca atctccccat
    11101 tacatattgt ataattaacc tttccatata aagatttacc tttaaatggt gaattatcag
    11161 ctttagaaac aaagctttca actgtccatt cttcttctgg attgaatata acaatgtctg
    11221 ctacagcccc ttcttctata aatccaatat ttaaattgta caactttgca ggatttatag
    11281 acattttttc tattaactgc atcatactaa gatgattttt atgcaccaaa tttgttattc
    11341 caagagctaa agaagtttca agcccaataa ttccacttgg agcttttata aactctctat
    11401 ctttttcttc tttactatga ggtgcatggt ccgttgctat tatttcaata gtaccatcat
    11461 ttaatccctc tataatctta tctctgtccc atttacttct tagaggtgga ttcatttttg
    11521 catttgttcc aaactccaat acatcttcct cagtcaatgt aaagtgatgt ggtgaagctt
    11581 ctgcaactac atttgcacct aagtattttg caaatctaac catatcaaca gatacacctg
    11641 aacttatatg ttgtatatca actttagcac cagtctttag tgccaacata caatctctgg
    11701 ctaccataac atcttctgct acacttgatg caccttttaa tcctagttct gaagctacct
    11761 ttcctgcatt tacacctgga tttccaacta aagaagggtc ttcttcatga aaactaagtg
    11821 gtacatccaa ctcttttgcc ttaatcattg cctccataat tagattgcta tccattaaag
    11881 gtaatccatc atcagtaaac cctggtacac cagctttttt aagagcttcc atatcaacta
    11941 actctttacc ttcaaaacct ttagttatag ctgctgcttg taaaacattt atacatgcat
    12001 tttttgactt ttctttaaca taattaaaag tatcttcatt atctacaata ggattagtat
    12061 ttgccataca tataacagtt gtaaatccac ctctagcagc agatttagct ccactttcta
    12121 tatcttcttt atatgtaaac ccagggtctc taaagtgtac atgtacatcc actaaacctg
    12181 gagcaactac attaccacta gcatctatta ttttttcata ttcatcactt tcatcaaact
    12241 tgcctatttt ataaatttta ttttctttta taattaagtc aatattctca tctctttgac
    12301 ttttagggtc tattactcta ccatttttaa ttaaaatcat attatttctc ctttatattt
    12361 ttaatttata tagactcata taatattata cattatttta catacttaaa tacaaaatta
    12421 cgtttgacat caatagcctt tttaggacaa agttcatggc aacagtaaca tcttatacat
    12481 ttacttaagt ctataatagc acccttagtc cctatctcta tagtggatgc aggacaagct
    12541 tccttgcatt ttccacattt tatgcaatct tgaaatctta ccacaggctt tggtgttata
    12601 agctttgaaa taggttcatt taaaaatttt ggtagcttta cagaaaacaa tcttaaatcc
    12661 ttacttacat ttggaatctt gtaatttttt ataattaaat cttttatatc ttcacctaaa
    12721 atttcaatat ctgaaaaatc ttcttgtata tatcctctct taatagcacc tctaattgta
    12781 gcaactctat ttggaagtaa acttataatc ttacatgcaa caacatctat agcatatgga
    12841 ttttgtgatg ctaataaaac tcctatttct cgagtagtac cagctgaagg tccatcacct
    12901 tccataccta ctataccatc cataattgtc aatgttggtt ttatataatc acaaatatct
    12961 aaaagtacat tttcgcaaaa caactcttct gtaggaaatc tataatgtat ctctgctttt
    13021 ttcaatccag ctatgcatcc atataagttt tttgtaccac cagtaaaagt tgccattata
    13081 tgagtcttta atttacaaag atttattata taatctgaat ctactattgg tttaataata
    13141 tccatatatt ttagaacatg tgctttttca ctatgtatct taacatctga aacatcataa
    13201 tttaacttaa tgcccagttc atttgctact tcttcaattc cagatgcctt ataaatgctc
    13261 ttaagagaac tttgattata tggaccacca gggctatctg ctattgtaat atcacaattt
    13321 aactctaaaa gtttttcaca tacaactttt acaaccatag gatgtgtagt tgtagcttct
    13381 tcaggctttt ttttcattaa taaatttggt tttataagta ctttgcttcc ttttttaaca
    13441 aacttatcta taccaccaat atcctctaaa tttttttcaa tagataactt tacttcttca
    13501 taattgtaag ttttacaact cctaacagat accgacttca tattatctcc atccttctat
    13561 cacaacagtt tatttgtata atttaaaaaa ggagaaacca aagtttctcc ttttaatcta
    13621 tgttttaatt gttttaatta ttattttcca aaatgtctgt ttaataatcc taagaatgct
    13681 ttaccatgtc tagcttcgtc tttacacatt tcatgtactg tatcatgtat agcatctaat
    13741 cctaattctt tagctctctt agctaatttt aattttccat cagttgcacc atactcagca
    13801 tcaactctaa ctcttaagtt ttcttttgta tctgcaacta caacttctcc aagaagttct
    13861 gcaaatttag cagcatgttc agcctcttca aaagctattc tcttatacgc ttcagctact
    13921 tctggataac cttctctatc agcttgtcta ctcattgcta aatacattcc tacttctgta
    13981 cactcaccag taaagttagc tcttaatcct tcgattattt ctgcatctac accttgagct
    14041 actcctattc tatgttcatc agcccaaacc atttcgcctt tcatttcttc aaatttatca
    14101 gctccaactt tacatactgg acattgtgct ggtgcagcat ctccttcatg tatatatcca
    14161 catactgtac aaacaaattt tttcataatt aatttcctcc caattttata taattaaatt
    14221 tttattttta agtttaataa cttgctacaa gaatatatat accccagact ttatagctta
    14281 aacactaaaa ataaaattta tttcctaatt aattttatca ctttgaaaca ccagtatcaa
    14341 ccaagttttg atgtcaaatt tctacataat acataattta tttgattaat taggaaatag
    14401 ttgtcatatt aaatactttc taatgaagac tcctctgaat ctttaagttt cacttttttg
    14461 ctctttaaaa aagataattt agtttctgca gttatgattg caacaaatat caagatacta
    14521 ccaataacca attttgctgt aataagttcc tttagaatta ttatagaaaa tattgtacca
    14581 aatacagcct ctgtggataa tattatagct gttctagttc catcaactct tttttgacat
    14641 atagtctgaa ataaaaatcc aattgtagta ctaaatacag ctagatacat agttcccatg
    14701 taaccagata attcagcttc catctttaat tgacctgcaa atgtttgaac tacaacagac
    14761 attaaaaacg ctactgtaaa ttgaatagct gtcaatgcca ttgggttgtt atcttttgca
    14821 aactcacttg taaagaatat atgaaatgca aatccgaatg aacatataag tgttagaaaa
    14881 tctccaaaat taatagagaa atcagcttct aaagaaagta tcccaattcc tataagagct
    14941 accaaactac ttattattcc tattttatca agttttcttc tataaagtat aaatcctatg
    15001 aatggaacta ttacaacatt agctgctgtt ataaacgcat ttttagatgg tgtagtgtac
    15061 ataagtccaa tagtttgtac tgtgaatgct agaaataaaa atattcctaa aatccctcct
    15121 gcttttaata attttttacc catattggca cgaatctgtt taaaaaacag taaattcata
    15181 attatagctg ccaagaaaaa tcttaaagtt atgatttgta gaggtgtaag cccaccgtct
    15241 aatgcaaatt gggttgctac aaagccactt ccccatataa ttgctataaa tattagtcct
    15301 atttctccaa agtatttttt caataaagcc ccctcctttt tatttttttg caaaattata
    15361 aaccccctaa acagcaagag agtttttcta taagcattac tataaattaa tatagtaaat
    15421 tttttcagta ttttaaatct aaatttacat acattttgag ctcaaattta tatccactat
    15481 ttaacagtaa tatattaatc attttttgta aacacctttt taataaattt ttatattttt
    15541 tatatttttt tacctatact aaattttttt atagatattt ttaatatata tgatacttta
    15601 atattatgca aataatgtca tataagttac actttagtta tcttagttac aactttgtcg
    15661 gtttactttt tctactttct tcgatataat tataatatat ttttgataca attcgacaaa
    15721 agggggaatg ctgtaattat ggtaaaaaaa agaaaaaaga aaactgttgc acttactata
    15781 cttgttgtaa tagttattgg tattttttct atggcatggg cggacatgtc tagaaaaaaa
    15841 gaaatgaaag aaactcaaga aagacttaaa gctgaaagat tagaaaaaga aagaattgaa
    15901 aaagaaaaaa atgaacctat tattggtgca aaaaaagaag ctgtaaaaga atatggatat
    15961 gacgcaaaaa tagtttggga caaacttaac aaatacgact attcaaacaa tggagaaaag
    16021 atagtctttc ttacatttga tgatggacca tctactacta atactccaca agttctagat
    16081 atcttgaaaa gacatggtgt tagaggaact ttctttatta agggtgattc tcttgaaaga
    16141 aaaggagcta atgaaatttt aaaaagaaca tttgacgaag gaaatgcaat agcccatcac
    16201 tcttatactc atgactataa aaaactatat cctaacagaa gcctaaatct cgatacattc
    16261 gtaaatgaac ttaacaaaac tgatgaagct atgaaaaaag tcttaggaaa gaatttttcc
    16321 tcaaatgtag ttagatgccc aggcgggtat atgtcatgga aaaatatgga acctctagga
    16381 aactacttaa aagagaaaaa catggcatca attgattgga acgctttaaa tgctgatgca
    16441 gaaggtaaaa agaaaaatgc acaagaatta tttgaacatg caaagaaatc ttctgaaggc
    16501 aaagaaatgg ttgttctttt aatgcatgat acttatggaa aacaagaaac agtaaatgca
    16561 ttggaccaaa ttataacata ttttaaagac aatggttatc aatttaaaat tttaatataa
    16621 taaaaaagca agtttattga atgtacattt tatactcaat agacttgctt ttactttact
    16681 ctattttact agctcgattt ttacaaattt tttctttcct atttgaacaa ctaattcact
    16741 ctcttttaca atagtgctta aatcctctac tttttcacca tttaccttta ctccaccttg
    16801 agaagctaat cttctaactt cactcttact cttaacaatc tcattagaaa ctagtacttc
    16861 gattaagtca aatccatctt ccttaacttg aattgtttgt atatcttcag gtatttgacc
    16921 tttttggaat acagatttaa atctttcttc agcttcttta gcagattctt ctccatggta
    16981 taatgttact atttctcttg ctaagtccat ctttatatct cttggattta ctgaaccctc
    17041 tttcaactga gattctattt tattaacttc atcaggatga acatctgtca ctaaattgta
    17101 gtatttaatt ataagttcat caggaatttc catagatttt tggtacatta ttcccgcttc
    17161 ttcatctatt ccaatatagt tacctaaact cttactcatt ttttctttac catctaatcc
    17221 ttctattaaa ggcatcatta ttactatttg agactccata ccaaattctc tttgtaatga
    17281 tcttcccatt agtaaattga atctttggtc agttccacca agttctatat cagcttctaa
    17341 tgctatagag tcatatgctt gcattaatgg gtagaagaac tcatgtactg atattggcat
    17401 ttgtccttca tatctctttt taaaatcttc tctttctaac attctcgcaa cagtaatagt
    17461 tgctgctaat tttattacat cttcaaaatt taacttagct aaccactcgc tattaaatct
    17521 tacaatagtt ttttctttat ctaaaacttt gaaaatttgt tcttcataag tttttgcatt
    17581 tgcaagaact tgctctgttg ttaaagcttt tctagcttta gattttccag ttgggtctcc
    17641 aatttttcct gtaaaatctc caattattat tactatttga tgacctaaat cttgtaattg
    17701 tttcatcttt ctaagaacta ctgtatgccc taaatgtata tctggagcac ttgggtctag
    17761 acccaatttt actatcatag gttttccttc tttttctgat ttaataagct tttcccttaa
    17821 ctctttctca tctattaaat catctacacc tttcataatt attcgcattt gctcgtctat
    17881 cgatttcatt tttcattcct cctaaaatat ttatataatc tataattttt atttttataa
    17941 taaaaaaagc tttcatcctc ttaaaaggac gaaagcattg cttacgtgtt accaccttag
    18001 ttcatcaata tctcacgata ttaatctctt agagtacaat tacatatact cgtgtactat
    18061 aacgtgtact aatccgatat ctcttaatgg ttgttatctt tcagagatag tgctctgaga
    18121 tgtattcaaa ctaaatcaat ttattccatt tcaccaaact ggaactctct gttaaataaa
    18181 atctagccct acttcttctc ttcatagcat ttatatattc agattatttc atcaattaca
    18241 agaaattttt cttaacttac tcataatata ttattatttt tatattttgt caaccttttt
    18301 tattattata tacatgtttt tttacattat actacttttt tatatttttt accaataccc
    18361 aaaatttatt tttcaatcaa aagatttatt tgtttattga ctatattact ctgactcaat
    18421 cttatattta ttttcttatt taaaaatgcg tttataatta ttaaaccaat aactgttaca
    18481 tcaaatataa aaaacagcaa taaaattatt atcaaaaata aaattctagc atataataat
    18541 tttctaaact tagctattat aaagttttgt agtgacatac atgagtctct aatgataaaa
    18601 tagacactta tataggttat tgatataaag attgttttta aataaacaga atcaacttta
    18661 acattcaaca gttttagaac ttcaaaaatg gctagataag aaacacatat gtaaaaagct
    18721 ttttttgagc aacataaaaa ttggtttaaa tttctaaaat tcttaaatat taaaaatttt
    18781 tttaaagaat tagaatctaa aatatgcaat ttattcccta aaattaaact taataaatat
    18841 atgcaaaaca ccataccaaa aggaaacaat gctatcatta aataataaat aaaatttgta
    18901 tacccattaa aataaatcca tttgctatat ccttgaaatt ctttcataaa actataacct
    18961 attaatgttg aagcatatat atcaatgaaa accattacaa taaccccaaa tactgaacca
    19021 tatactaaat catccataac cattgtaggc ttatttatta gatagccaca cattatacca
    19081 agtaatacac tctgacttgc ccaaatagca gttcctaaat ttccaagtac cagagaaatt
    19141 attaaaagcg aagtaattcc agataaaatg gtatacttta aatcacattt caagcagatt
    19201 atacaaaaaa atactggcac tataaaatct aagtaaatag catatccaaa tccagtactc
    19261 acagctataa ttgttactac tatgaataaa gatgacaata aagctgcttc agttaatttt
    19321 ttagtagtat tcaaaacact gcctccataa atcataaatt ttctaactaa agttttgttt
    19381 ttaatatagt atatcacttt ttaaaaatat ttttaataaa tataaatttt aat
//
LOCUS       DAELLV010000050        19125 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.050, whole genome shotgun sequence.
ACCESSION   DAELLV010000050 DAELLV010000000
VERSION     DAELLV010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19125
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.050"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            32..1144
                     /locus_tag="KPD58_001482"
     CDS             32..1144
                     /locus_tag="KPD58_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HBG0265259.1"
                     /translation="MNSRIKSIDIIRGLSIALMIVCNNPGTWMRMYPQLRHAVWHGVT
                     LADFAFPFFVISLGVTIPISINSKLKNNKSTLSIILSIFKRSILLILFGFFLNYLGNP
                     DLDTVRILGVLQRMGLVYFVTSLAYLLLKKLNVGSTATIITFLYIATFIIVGYYILAK
                     PYGFELEGSLAQLVDLHFFKGHLYKPEFEPDGFLTSIVAISSGMLGCTMGCVLLKENL
                     GEYKKFFKILVMSIILLIGAFIFNQYFPFNKRLWSSSFVLLMAGSYGILLSIFYFICD
                     IKNKSKIFTPIIALGSSPIFTYMCLEILSHVFWNVPKLTNKVDYPTTLVEWTTYELIT
                     PWAGTTWDSLIFSLLYVLFWVIVMSIMYKKKIFIKI"
     gene            1398..3422
                     /locus_tag="KPD58_001483"
     CDS             1398..3422
                     /locus_tag="KPD58_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG0265260.1"
                     /translation="MKKTTKLLATGMLSVAMVAPNVALAAENTTANTESNSDININLQ
                     RKSVVLGSKSNASVKFKEKLNADSITLNFMCYDMPLEATLNYNEKTDSYEGVINYNKD
                     PEYLNVWELQSIKINGKDEQKVLNKEDLESMGLNLKDYDVTQEFIISDANSTKAVNEY
                     MRKTSAPVKKLAGATRFETAVEISKQGWKDGSSKVVIVNGELAADGITATPLASTYDA
                     PILLANKDDIPESTKAELKRLNPSDVIIIGDDGSVSQKAVSQIKSAVNVNVTRIGGVD
                     RHETSLLIAKEIDKYHDVNKIYIANGYAGEYDALNISSKAGEDQQPIILANKDSVPQG
                     TYNWLSSQGLEEAYYIGGSQSLSSKIIDQISKIAKNGTSKNRVSGADRHETNANVIKT
                     FYPDKELSAMLVAKSDIIVDSITAGPLAAKLKAPILITPKTYVSAYHNTNLSEKTAET
                     VYQIGDGMKDSVINSIASSLSKHNAPTEPDNSGSAAGKTVVIDPGHGGSDSGATSGLN
                     GGAQEKKYTLNTALATTEYLRSKGINVVMTRDTDKTMALGERTALSNTIKPDLFTSIH
                     YNASNGAGNGVEIYYKVKDKNGGTTKTAASNILKRILEKFNMKNRGIKTRTLDNGKDY
                     LYVLRNNNYPAILVECAFIDNKSDMDKLNTAEKVKTMGTQIGIGIEDTVK"
     gene            3642..4328
                     /locus_tag="KPD58_001484"
     CDS             3642..4328
                     /locus_tag="KPD58_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBG0265261.1"
                     /translation="MSGYTNDECEIPKIKSYPGADKEIASEIDYSIVKGTVLFDLYQR
                     IMDLVLSIIGLVIGLPLIAIFGILIKIEDKGPITYKQERLGKCGRRFYIYKLRSMRTD
                     AEKFGAQWAEKDDPRITKVGKFIRKTRIDEIPQLFNILKGDMGLIGPRPERPNFTVQF
                     NEEIPGFINRLAIKPGLTGWAQVNGGYEITPEEKLKEDIYYIKNRSILLDFKILFKTV
                     KVVLTGDGAR"
     gene            4423..5478
                     /locus_tag="KPD58_001485"
     CDS             4423..5478
                     /locus_tag="KPD58_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp7"
                     /protein_id="HBG0265262.1"
                     /translation="MIKKISTILSLVLLISISSTIGVFADANPKRELIEGSIPEISTE
                     LNKRAFKDSKEVILVNEESIVDSISATPLAYAKNAPIVVTKSKNLGRVTRNYLKELGP
                     EKVTIVGGLKAVSKDAERNIEKMGMKVERIRGKDRYDTSLKIAREMYRTVGFDEAFLL
                     SSTTGLENAISVYSYAAKSGMPIIWAKDEGFEEQIDFLKGKNLKKIYALGDSKEFIAE
                     IDSNLKNIEGIKQINKSSTNVDLIKKFYDEKDIKKIYTARLDFGSRSDVNEYISLGVV
                     SAKENMPILICSDNLSRAQDKFLKDSNINDVVEVGYTVGDYSLFKSIFNLTFLSCIVL
                     ILLLLLITFRALRYESK"
     gene            5593..7149
                     /gene="murJ"
                     /locus_tag="KPD58_001486"
     CDS             5593..7149
                     /gene="murJ"
                     /locus_tag="KPD58_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBG0265263.1"
                     /translation="MSKTAKAALWIMAATMFSKVLGFLRELVLANFYGTGMYADVFVL
                     TLNIPGLIIAVIGSAVATTYIPMYFETKKRLGDEGALKFTNNVLNICYIMAIVIAIIG
                     LLFTEQFVTVFAAGFRNDPAKFQAAILFTKIMISGVLFLSGSKIFSSFLQVNDSFVIP
                     GLIGIPYNIIIIAAIALSAGKNVWILPAGALLAMASQLLFQLPFAFKKSYKYKPYINL
                     KDESIKELVNLVLPMLVGVAVGQLNIFVDRLLATTLGDGKLSALNYANRLNEFVMALF
                     VTSIITVIYPKLAKMSGKDNKEGFISTIVKSSNCIILVVLPISIGAIILAEPLVRILF
                     QRGKFDALSTDLTSIALRLYSLGLLACGVRDVLYRAFYSLSDTKTPMINGSIALIINI
                     VLNLILIRPLGHAGIAISTSTSNIITVILLFISLKKKNGYFGGDKIIKTGLKSLVASG
                     VMAVATLLIYNNLYAFMGSGTIKEIISVGAGVLGGASVYTVLIVLFKVEEMDLAFEFL
                     RKGKQKLLRR"
     gene            7195..8901
                     /locus_tag="KPD58_001487"
     CDS             7195..8901
                     /locus_tag="KPD58_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBG0265264.1"
                     /translation="MDYKNNYEMWLNSPYFDEQTKNELLSIKDDEKEIQDRFYKNLEF
                     GTGGLRGIIGAGTNRINIYTVRRATLGVLNYIMKTQGEEGKQKGIVIAHDSRYMSREF
                     CIEVAKTLSAYGVKAYIFEELKPTPELSFAVRYLKCAMGIVITASHNPKEYNGYKVYD
                     SDGGQICIDMANDIIAEVNKIDDYSTIKSIDFEEALSKNLITILDNEVDDEFIKAVKK
                     QVLRQNIIDEYGKKLKIIYTPIHGTGNKPVRKVLNECGFENVMVVKEQELPDSNFSTV
                     KYPNPEEKSVFNIAIEMAKNNGTDLIIGTDPDCDRVGIVVKDSSGEYVVLNGNQVGSL
                     LVRYILESLVEENKLPKNNPTIIKTIVTSELGAKIAKAYNVDCLNTLTGFKFIGEKIK
                     AFEESNDRSFIMGYEESYGYLIGTHARDKDGVVSSLMICEMAAYYSSKGMNLYEALID
                     TYNKFGYYKEDLKSVTLKGIDGIKKIKEMMLYFRSVKIDNVADVKVDKILDYKDGVDD
                     LPKSDVLKFLLEDGSWIAIRPSGTEPKIKFYFGANSDNQEDVEFKLNNLISYILNVVD
                     SI"
     gene            8923..9996
                     /locus_tag="KPD58_001488"
     CDS             8923..9996
                     /locus_tag="KPD58_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HBG0265265.1"
                     /translation="MKVYNVIMAGGGGTRFWPLSRQEVPKQLINLSGEDALINETINR
                     IDSLAKKDDLFIVTNEKQLEALKDIVKDKCLDSNILPEPCARNTAAAIGFAAFNIMKK
                     YGDGVMCVYPADHYIKDEKEFKSILEKAIYIAENNDKLVTIGITPTFPSTGYGYINFN
                     RENTIEDVAYEVVEFVEKPNYEIAKEYVNSKKYVWNSGMFVWKVSKILEDFKRYLPKV
                     YEKLEDISKYLGTKEEMEKIKEIYPTIQSISIDYGIMERSNDVIVVPGDFGWNDVGSW
                     DSLGAIYPTDDEGNIKRGENITIDTKNSIIYSDDKLISTIGISDLIVVSTNDAVMVCR
                     KDKAQDVKKIVEQLKEEDRQEYM"
     gene            10008..10730
                     /locus_tag="KPD58_001489"
     CDS             10008..10730
                     /locus_tag="KPD58_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG0265266.1"
                     /translation="MIPKKIHYVWFGGPKGNIENICINSWKEKLPEYEIVEWNEKNFD
                     IEKEIKGNKFLEECYKRKLWAFISDYTRIKVLYEQGGVYMDTDMQILKDITPLLENNR
                     LVCGYEDDREYINGAIIGVEKGHPFLKDLLEYYEKEVLTSSLFTIPKIMTHLMEKNYK
                     KIDPNNYEEGIRVYDKEYFYPFGFKEDFTPECITENTFGIHWWGKSWAKKRNYFLESK
                     HLTGVNKIWKCCKIFASNTLRS"
     gene            10746..11903
                     /locus_tag="KPD58_001490"
     CDS             10746..11903
                     /locus_tag="KPD58_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide polymerase"
                     /protein_id="HBG0265267.1"
                     /translation="MKTLNDDRNIMIRKYLYIFVLFIILLRKYMISFIDPNMDIGMIK
                     SALFYSSIGILMLLFLFDKRKSITEMVLVGVCVLLYLLNREGAILLIVLLAVSAKQID
                     DKFIVKNYLIISACFLMVAILLFNLFPSLIFNQEVHYRYIEKIDMLVTRMDFGLGNPN
                     SVFYHMVTIYAAYIFLRYKDYNKWDRILLFGSAFFVYQTTYSRTGFFTILAGLIFVEI
                     IRWIDIKKIKGLPMLLKTLPVILTLFSVIIGTVFDKSTLLNRLLASRPKFWNVYLAEK
                     GNFLKPFGNSYSPAIKATNPLDSSYVYIISVLGVVACILFMYLMYKGIESFIEKDKKA
                     YLVALFIFLLYSFAENLLLEASFSFAVVLLIKEVILNDKREIDLWKMKSRR"
     gene            11906..12745
                     /locus_tag="KPD58_001491"
     CDS             11906..12745
                     /locus_tag="KPD58_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG0265268.1"
                     /translation="MLISLIMPTLNRYDDIYLLMDSLENQTYKNFELIVVDQNDNSKV
                     KEIVDKYIDKLDIKYIKSSKKGLSYNRNVGIDNAVGQIIGFPDDDCVYENDTLEKVIN
                     FFNKNKDYKIYSCKTMDSNKVDAFKKMYDGTCDITSSNVLDTITSITFFIDFEGKDYT
                     RFDEKLGVGGEFGAGEEIDYVLNLLSLGFKGKYFGNDIIYHPAKKHSKSKEKYQKDYN
                     YGRGFGALCKKEIVYRKNYKFAKVMVSKLVRNIGGLILSSNRDYHSATIKGRINGFRQ
                     YKL"
     gene            12760..13935
                     /locus_tag="KPD58_001492"
     CDS             12760..13935
                     /locus_tag="KPD58_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HBG0265269.1"
                     /translation="MDKVKFLINMLLAFFIYPFNKHKFNGRDIWLIGGHSGDIYNDNS
                     KFFYEYMLKEHNDVETYWVVNKDSKVFDKIPGKKLIRGSVENYLYYYNSKAIVFSHAP
                     SADIAPYNFAVPVLNYFHKKTIKVFLNHGTISFKKRKPMNKKFKNIIDNLYKSYNIVT
                     ASSEFERNVMVNDWGMLDDSVYIIGNARYDNLPTNEVAQTRDILYTPTWRDWIKFSSG
                     KFTDTDYFKNIMNFLNDDKLNKILDEKDINVKIYMHHLMHEFIDDIKENITGKRIVFL
                     DKGVTLANEIRKSAANITDYSSVAIDFLYMNRPILFYQFDLDEYMEKVDSYIDLKSEM
                     FGSLAYNNDEAVNKLIDIIENNFEVMDNQKNERNKFFRYNDNKNCKRIYDCVLSKIK"
     gene            13964..14713
                     /locus_tag="KPD58_001493"
     CDS             13964..14713
                     /locus_tag="KPD58_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG0265270.1"
                     /translation="MKKNLVSIITPMYNSEKFIEATIKSVLNQTYQEWEMLIIDDCST
                     DNSPNIVKSYMQQDSRIKCIKTETNKGVSNARNLALSKATGQFIAFLDSDDQWNSSKL
                     EKQVNFMLENDYVISFTSYELMDENDKKLNKVIKVPPNVDYRRLLKGNILGCLTVVID
                     KSKLDFEIRMSGVRHEDYVLWLSILKKGHIAHGINEVLALYRKSSNSLSGNKIKAAMW
                     TWNIYRNIEKIPLYKAIYYFINYGINGIKKS"
     gene            14735..15643
                     /locus_tag="KPD58_001494"
     CDS             14735..15643
                     /locus_tag="KPD58_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG0265271.1"
                     /translation="MYKYKFTVFTPTYNRAHLLENLYNDLKAQTYDFNDFEWLIVDDG
                     SSDSTKELVEKFISENKLNIRYIYKENGGKHTAINLGVKNADGELFFIVDSDDGLIKD
                     SLEIANDEWDSLENKEGFSGVVGLCVYPSGDLIGTEMPEDKKICHFADLYFKYGVKGD
                     KSIVFVTDELIKFPFPERKEVRFLPESVVWNEMSKYYKVICVNKPMIIRDYLDDGLTK
                     NILSKNALRGRALEFLYLINQNTYPLSRYPYMWIKNYINLARYSLLSDSHYFGELRKV
                     SDKLLYLALFPLGYYKYIGQRKLVSK"
     gene            15691..16443
                     /locus_tag="KPD58_001495"
     CDS             15691..16443
                     /locus_tag="KPD58_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG0265272.1"
                     /translation="MNEPLVSIITPVYNSEEFLSETIKSIQNQTYKNWQLLLVDDCSK
                     DNSSEIIKSFRKEDARIKYIKLEKNSGAAVSRNVGIKNAEGRFIAFVDSDDLWDSRKL
                     EIQIEYMLKENVGFSFTSYRYMRQDGSKTNKVARAPKKIDYEGLLRNTIIGCSTVVID
                     KEIVGEFSMPLVRRGQDTATWLQLLKKEKYAYGIQEDLVNYRLVGNSISSNKIKALKR
                     TWNTYRNVENLSLPKSLYVFCFYVFNAIKKRV"
     gene            16397..17764
                     /locus_tag="KPD58_001496"
     CDS             16397..17764
                     /locus_tag="KPD58_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HBG0265273.1"
                     /translation="MYFVSMFLMPLKKEYKFYILGGKKMKIAIAGTGYVGLVTGVCLA
                     EVGNENVVCVDVDEQKVKSMKLGIAPIYEEGLEELMVKNYNRGRIDYTTDYKKAYEDV
                     DVILIAVGTPERSDGSANLDYIKAVAKQIAETVTKDTLVVIKSTVPIGTNEEIEDYIN
                     RNLKNDVKVELASNPEFLSQGTAVKDTLHASRIVIGVESEWAREILEEIYKPFNQPII
                     VTNRNSAEMIKYASNNFLALKISFMNDIANLCEIAGANIEDVALGMSYDDRIGSKFLK
                     AGIGYGGSCFPKDTKALHWLSEHNGYELKTVKAAIEVNESQKLKLVRKASKLVESFQD
                     MKVAVLGLTFKPDTDDIREAPSIPNIKYLLERGARVTVYDPIGVNNTKKIFGDSILYA
                     DSVDDAIEDAEICFIMTEWKDVLNYDIHKYKEKMKNPLVLDGRNCYKIKDMEELGIEY
                     YSIGR"
     gene            17770..18861
                     /locus_tag="KPD58_001497"
     CDS             17770..18861
                     /locus_tag="KPD58_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG0265274.1"
                     /translation="MKTVCYLADGSNPHTLKWCSFFKNKGYDIHVISLNGGHMEGIKI
                     YNFGSNVEELKNENISKKTGYLGSIKQIKKLVNEIKPDILHAQYASSYGFIGSLLGYH
                     PYIISVWGTDIYDFPNNGFIQKQIIKHNFRKADYIFSNSRDMAKEANKYTAKHVDVTF
                     FGVDMDRFKPMEVEKEDAFVIGIIKSLEKKYGIEYLIQAFKMLKDEYKDKKIILKIGG
                     SGSQMDNLINLAKELGIENDVQFLGRISPENVSKTFNSFDVTVFPSLREGFGVAAIES
                     EACEVPVIVTNVGGHPESVWENETGLIVEPKQPEEIKNAIIKLMENDELRLNMGKKGR
                     QFVRENYEVNLNFNDIEKIYDSIFDKYKK"
     gene            18945..>19125
                     /locus_tag="KPD58_001498"
     CDS             18945..>19125
                     /locus_tag="KPD58_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265275.1"
                     /translation="MRESIDLEKESFFNRLFKKYKFRHLTLLILDICSVLVAFRMSLS
                     LTGNLNAFTTNDIIIS"
BASE COUNT         7599 a         1990 c         3509 g         6027 t
ORIGIN      
        1 aatttcaaat aatgctgtag attctaaact tatgaatagt aggattaaat caatagatat
       61 aataagaggg ctatcaatag ccctcatgat agtctgcaat aatcctggga cttggatgag
      121 aatgtatcct caattaagac atgctgtatg gcatggtgta acacttgcag attttgcatt
      181 tccttttttt gttatatctt taggtgttac aattccaata tcaataaata gtaaactaaa
      241 aaataataaa tccacactga gtataatact tagtatattc aagagaagta tcttattgat
      301 attatttgga tttttcttaa attacctagg aaatcctgat ttagatactg taagaatact
      361 tggagtactt caacgtatgg gattagtcta ttttgtgact agtttagcat atttactgct
      421 aaaaaaatta aatgttggaa gtactgctac aataattact tttctatata tagctacatt
      481 tataatagtt gggtactaca tcttagcaaa accttatgga tttgagctag aaggttctct
      541 tgcacagtta gtggatttac atttcttcaa aggacatttg tacaagccgg aatttgaacc
      601 agatggtttt ttaactagta tagtagcaat ttcttcaggt atgttgggat gcactatggg
      661 atgtgtactt ttaaaagaaa acctaggaga atataaaaaa ttctttaaaa tactggttat
      721 gagcataatc ctgctaatag gtgcttttat ctttaatcag tattttccat ttaataaaag
      781 attatggtct agctcatttg tacttttaat ggctggcagt tatggtatat tactgtcaat
      841 attttatttt atatgcgaca tcaaaaataa aagtaaaata tttaccccaa taatagcact
      901 tggaagtagc cccattttta catatatgtg ccttgagata ttgagtcatg ttttttggaa
      961 tgttccaaaa cttacgaaca aagtcgatta tccaactacc ttggtagagt ggactacata
     1021 tgaacttata acaccttggg caggtacaac ttgggattct cttatatttt ctttattgta
     1081 tgtcctattc tgggttatag taatgtctat aatgtacaag aaaaaaatat ttattaaaat
     1141 ttagtgcaat atttttagat gataaagtct ttgatttgtt caaatataca gaaactatca
     1201 aatataatca aaatttctcc tcgataaaat ttaaaaacca tgccataaaa gattacaaaa
     1261 agtttacaat ttgtaatttt ttgtgctata atgaaaaaag atttacataa tattgaattt
     1321 atgtaatata aaacaagctt aagatgtatt aaaaaagatt taaagattta aagaaccaaa
     1381 aaattagggg ggatatgatg aaaaaaacaa caaaattatt ggcaacagga atgttgtctg
     1441 tagcaatggt ggcaccaaat gtagctttag cggctgaaaa tactactgca aatacagaat
     1501 ctaattcaga tattaatata aatttacaaa gaaaaagtgt agtgttagga agtaaatcta
     1561 atgcaagtgt aaaatttaaa gaaaaattaa atgctgattc aataacatta aattttatgt
     1621 gttatgatat gccacttgaa gctactttaa attacaatga aaagacagat tcatatgaag
     1681 gtgtaataaa ctataacaaa gacccagaat acttaaatgt atgggaactt caaagcataa
     1741 aaataaatgg taaagatgaa cagaaagttt taaacaaaga agacttagag tctatgggat
     1801 taaatcttaa ggattatgat gttacacaag agtttataat aagtgatgca aattcaacaa
     1861 aagcagtaaa tgaatatatg agaaaaactt ctgcaccagt aaaaaaactt gctggagcta
     1921 ctagatttga aactgctgtt gagataagta aacaaggttg gaaagatggg tctagtaagg
     1981 ttgtaatagt taatggagaa ttagcagcag atggaattac agctactcca cttgcatcta
     2041 catatgacgc accaatatta cttgcaaata aagatgatat accagaaagt acaaaagcag
     2101 agttaaaacg tctaaatcca agtgatgtaa taattatagg tgatgatggt tcagtttcac
     2161 aaaaggcagt gtctcaaatt aaatctgctg taaatgtaaa tgtgactcgt attggtggag
     2221 ttgatagaca tgaaacatct ttacttatag caaaagaaat agataagtat catgatgtta
     2281 ataaaatata catagcaaat ggatatgctg gtgaatatga tgctcttaat atttcatcaa
     2341 aagctggtga agaccaacaa cctataatat tagctaataa agattctgta ccacaaggta
     2401 catataattg gttatcttca caaggtttag aagaagctta ttacataggt ggtagtcaaa
     2461 gtttatcatc aaaaattata gaccaaatat ctaaaatagc aaaaaatggt acttcaaaaa
     2521 atagagtttc tggagcagat agacatgaaa caaatgcaaa tgtaattaag actttctatc
     2581 cagataaaga gttgagtgca atgttagttg caaaatctga cataatagtt gattcaataa
     2641 cagcaggacc tttagcagct aaattaaaag ctccaatact tataactcca aaaacttatg
     2701 tttctgcata ccataataca aatttatcag aaaaaacagc agaaacagta tatcaaattg
     2761 gtgatggaat gaaagatagt gttataaatt ctatagcatc tagtttatca aaacacaatg
     2821 caccaacaga accagataat tcaggttcag cagcaggaaa aactgtagtg atagacccag
     2881 gtcatggggg ttctgattca ggggcaacaa gtggattaaa tggtggagca caagaaaaga
     2941 aatatactct taatacagca ttagcaacta ctgaatattt acgttcaaaa ggtataaatg
     3001 ttgttatgac tagagataca gataaaacaa tggctttagg agaaagaaca gcattatcaa
     3061 atactataaa gccagattta tttacaagta tacattacaa tgcgtctaat ggagctggta
     3121 atggtgtgga aatttattac aaagtaaaag ataaaaatgg tggaacaact aagactgcag
     3181 catcaaatat cttaaaaaga atactagaaa aatttaacat gaaaaataga ggaatcaaga
     3241 caagaacact tgataatgga aaagattacc tatatgtgtt aagaaataac aattatccag
     3301 caatacttgt tgaatgtgca tttattgaca ataagagcga tatggataag ttaaatactg
     3361 cagaaaaagt aaaaacaatg ggaactcaaa ttggaatagg aatagaagat acagtaaaat
     3421 aaaatagaaa atattaaagc aaaacatatc atctcctgtt ataatgtaaa atataacaag
     3481 agatgatata tttttgtaaa gaatcaaatt gtttacaatg atgtttcttg tattgataat
     3541 ctgctcatta ttgagtataa tatagaaata agtcaaaaat aagatagaaa taagttgaaa
     3601 ttaagaatta aaaaaatttt tattccaata tggaggtaac tatgtctgga tatactaatg
     3661 atgagtgtga aatacctaaa attaaaagct atccaggggc agataaagaa attgcaagtg
     3721 agattgacta tagcatagta aaaggaacag ttttattcga cttgtaccaa agaattatgg
     3781 atttagtttt atctataata ggacttgtga taggtttacc tttaatagca atatttggaa
     3841 ttcttattaa aatagaggac aaaggaccaa taacttataa gcaagaaaga ctaggaaaat
     3901 gcgggagaag attctatata tataaattga gatctatgag aacagacgca gaaaaatttg
     3961 gcgcacaatg ggctgaaaaa gatgatccta gaataactaa ggttggaaag tttattagga
     4021 aaactagaat tgatgagatt ccacagctgt ttaatatatt aaagggggac atgggcttaa
     4081 taggtcctag accagagaga ccgaacttta ctgttcaatt taatgaggaa atacctggat
     4141 ttatcaacag gctcgctata aaacctgggc ttacaggttg ggcacaagta aatggtggat
     4201 acgaaatcac tcctgaagaa aaacttaaag aagatattta ctatataaaa aataggagta
     4261 tattacttga ctttaagata ctctttaaaa ctgttaaagt agtattgaca ggagacggag
     4321 ctagataatt ttaaaatatt agattactaa tcaatgaaaa agctctttaa aaagagcttt
     4381 tttattgtca aaaaatacaa aaaagaaaag gaatgagaac ttatgattaa aaaaatttct
     4441 actatattat cgttagtttt gttaatctct atttctagta cgataggagt atttgcagat
     4501 gcaaatccca aaagagaatt gatagaaggg agtataccag agatttcgac agaattaaat
     4561 aaaagagcat ttaaggattc taaagaagta attcttgtaa atgaagagtc aattgtagat
     4621 agtataagtg caacaccttt agcatatgca aaaaatgctc caatagttgt aactaagagt
     4681 aaaaatcttg gtagagtaac aagaaattat ctaaaagaac taggtccaga gaaggttacg
     4741 atagtaggtg gtttaaaggc agtatctaaa gatgctgaac gtaatataga aaaaatggga
     4801 atgaaagtag aaagaataag agggaaagac agatatgata catctttaaa gatagctaga
     4861 gaaatgtata gaacagtagg atttgatgaa gcatttttac ttagctcaac aacaggatta
     4921 gagaacgcca tatctgtata ttcttatgcc gctaaaagtg gaatgccaat aatatgggct
     4981 aaagatgaag ggtttgagga acaaattgat ttcttaaaag gcaaaaatct taaaaaaata
     5041 tatgcattag gtgattcaaa agagtttatt gcagagattg attctaactt aaaaaatata
     5101 gaaggaataa agcaaataaa taagtcaagt acaaatgttg atttaataaa gaagttttat
     5161 gatgaaaaag atataaagaa aatctatact gctagattag actttggtag tagatctgat
     5221 gtaaacgaat atatttcttt gggagtagta tcagcaaaag aaaatatgcc aatactgatt
     5281 tgtagtgata acttaagtcg tgcacaagat aaattcttga aagatagcaa tataaatgat
     5341 gtagttgaag ttgggtatac agtaggtgat tatagtctat ttaagagcat atttaatcta
     5401 acattcttat cttgtatagt attaatacta ttattactat tgattacatt tagggcactt
     5461 agatatgagt cgaaatagtt tttgatttat aaggaagctt ttcataggtt aaatttactt
     5521 aattagaata atgtttataa gcagttttaa aagttctact tttagagttt aaattagata
     5581 aggagaaaga agatgtcaaa aacagctaaa gcagcattgt ggattatggc agccactatg
     5641 ttttcaaaag tattaggttt ccttagagaa cttgtacttg caaatttcta tgggacaggt
     5701 atgtatgcag acgtatttgt attaacatta aatatacctg gtttaatcat agcagtcata
     5761 ggctctgcag ttgctacaac ttatatacct atgtactttg agacaaagaa aagactcgga
     5821 gatgaaggtg ccttaaaatt tactaataat gttttaaata tatgctatat aatggctata
     5881 gtaatagcta ttattggtct tttatttaca gagcaatttg ttacagtatt tgcagcagga
     5941 tttagaaacg accctgctaa gttccaagca gcaatattat ttactaaaat aatgatttca
     6001 ggagttttat tcctgagtgg aagtaagata tttagttcgt ttttacaggt gaatgacagt
     6061 tttgtaattc ctgggcttat aggaatccca tataatatta taataatagc agcaatagca
     6121 ctaagtgcag gtaaaaatgt atggatactg ccagcaggag cattattggc tatggcgagt
     6181 cagctattgt ttcaactgcc atttgcgttt aagaagtcct acaagtataa gccatatata
     6241 aatttaaagg atgagtctat aaaagagtta gtcaacctag tattgcctat gttggtagga
     6301 gtagctgttg gacagttaaa tatatttgtt gaccgattat tggcaaccac tttgggtgat
     6361 ggaaagttgt ctgctcttaa ctatgcaaat aggctaaatg agtttgttat ggcattattt
     6421 gttacatcta taataacagt tatatatcct aaactagcaa aaatgtctgg taaagataat
     6481 aaagaaggat ttataagcac tattgtaaaa tcttcaaatt gtattatatt ggtggtgctt
     6541 cctatatcta taggagctat tatattagca gagcctcttg ttagaatatt attccaaagg
     6601 ggtaaatttg atgcgttatc aacagacctt acatctatag cgcttagatt atattcacta
     6661 ggtttattgg cttgtggagt aagagatgtc ttatataggg cattttactc tctttcagat
     6721 acaaaaacac caatgataaa tggaagtata gctttaataa taaatatagt tcttaattta
     6781 atattgataa gaccattggg tcatgcaggt attgctatat caactagtac atcaaacata
     6841 attacagtta tattattatt tatatctctt aaaaagaaaa atggatattt tggaggggat
     6901 aaaattataa agactggtct aaaaagtttg gtagcatctg gtgtgatggc agtagctact
     6961 ttattgatat ataataatct atatgcattt atgggttcag gaacaattaa agaaattata
     7021 tcagttggag ctggagtatt aggtggagct tctgtatata ctgtattgat agttttattt
     7081 aaggtagaag agatggattt agcttttgaa tttttaagaa aaggaaaaca aaaactactt
     7141 agaagataga taattacctt gaaattttaa attagagtca gaaaggatga aaatatggat
     7201 tataagaata attatgagat gtggttaaat tctccttatt ttgatgaaca aacgaaaaat
     7261 gagctattaa gtataaaaga tgatgaaaaa gaaatacaag atagatttta taaaaattta
     7321 gagtttggaa caggtggttt aagaggtata ataggagcgg gaactaatag aatcaatatt
     7381 tatactgtta gacgtgcgac actgggcgtt ttaaattata tcatgaaaac tcaaggagaa
     7441 gaaggtaagc aaaagggaat agttatagca catgatagta gatacatgtc tagggagttt
     7501 tgtatagaag tagcaaagac tttaagtgct tatggtgtaa aggcttatat ttttgaagaa
     7561 ttaaagccta caccagaact ttcttttgct gttagatatt taaagtgtgc tatgggaata
     7621 gtaataacag caagtcataa tcctaaagaa tacaatggat ataaagtata tgactcagat
     7681 ggtggtcaaa tctgtataga tatggcaaat gatataatag cagaagttaa taagattgat
     7741 gattacagta ctattaaaag tatagatttt gaagaagctt tatctaaaaa tttaataact
     7801 atattagata atgaagtaga tgatgagttt ataaaagctg ttaagaaaca agttttaaga
     7861 caaaatataa tagatgaata cgggaaaaaa ttaaaaataa tatatacacc tattcatggt
     7921 acaggaaata agccagtaag aaaagtctta aatgaatgtg gttttgaaaa tgtaatggta
     7981 gttaaggagc aagaattgcc agattctaat ttttcaacag taaagtatcc taatcctgaa
     8041 gaaaagtcag tgtttaatat agctattgaa atggcaaaga ataatggcac tgatttgata
     8101 ataggaacag accctgattg tgatagagta ggtatcgttg taaaagattc aagtggtgaa
     8161 tatgtagtct taaatggaaa tcaagtcggt tcacttttag ttagatatat attagagagt
     8221 ttagttgaag aaaataaact tcctaaaaac aatcctacaa taataaaaac tatagttaca
     8281 tcagaactag gtgcaaaaat agcaaaggct tataatgtag attgtttaaa tactttaaca
     8341 ggatttaaat ttataggaga aaaaataaaa gcgtttgaag aaagcaatga tagaagtttt
     8401 ataatgggat atgaggaaag ctatggttac ttaattggaa ctcatgctag agataaggat
     8461 ggtgttgtat cttcacttat gatatgtgaa atggcagcat actattcttc aaaaggtatg
     8521 aacttgtatg aagctttgat agatacatat aataaatttg gatactacaa agaggactta
     8581 aagtcagtaa ctttaaaagg catagatgga ataaaaaaaa taaaagagat gatgctttac
     8641 tttagaagtg ttaaaataga taatgtggct gatgtaaaag tagataaaat tttagactat
     8701 aaagatggtg ttgatgattt acctaagtca gatgtactta aatttttact tgaagatggc
     8761 tcttggatag ctataagacc ttcaggtaca gaacctaaaa ttaaatttta ctttggagca
     8821 aattcagaca atcaagaaga tgtagaattt aagttgaaca atttaatatc ttatatatta
     8881 aatgtagtgg attctatata aaaagctaag gggagataaa caatgaaagt atacaatgtc
     8941 ataatggctg gaggaggagg aactcgtttc tggccactta gtagacaaga agtacctaaa
     9001 cagcttataa atttaagtgg agaagatgct ttaataaacg aaactataaa cagaatagat
     9061 tctctagcaa aaaaagatga tttatttata gtaaccaatg agaagcaatt ggaagcttta
     9121 aaggatatag taaaagataa atgtttggat agtaatatat tgccagagcc atgtgccaga
     9181 aatacagcag cagctatagg ctttgcagca tttaacataa tgaaaaaata tggtgatggt
     9241 gttatgtgtg tatatccagc tgaccattat ataaaagatg aaaaagaatt taaatctatt
     9301 ttggaaaaag ctatctatat agcagaaaat aatgataaac tagtaactat tgggataact
     9361 cctacatttc catcaacagg atatggatac ataaatttta atagagaaaa tactatagaa
     9421 gatgttgcat atgaggtagt ggagtttgta gaaaaaccaa actatgaaat tgctaaagaa
     9481 tatgtaaact ctaaaaagta tgtatggaat agtggaatgt tcgtatggaa ggtatctaaa
     9541 atactagaag attttaagag atacttgcca aaagtatatg aaaaattaga agatataagt
     9601 aaatacttag gaacaaaaga agaaatggaa aaaataaaag aaatataccc aactatacaa
     9661 tcaatctcta tagattatgg tattatggaa agaagtaatg atgttatagt agttcctggt
     9721 gattttggat ggaatgatgt tggttcttgg gattctctag gtgcaatata tccaactgat
     9781 gatgaaggta atatcaaaag aggtgaaaat attactatag atactaaaaa ttcaattata
     9841 tattcagatg acaaattaat atcaacaata ggtattagtg atttgatagt agtatctaca
     9901 aatgatgcag ttatggtttg tagaaaagat aaagctcaag atgtcaaaaa aatagtagag
     9961 cagttaaaag aagaagatag acaagaatat atgtaggagg tgttcatttg attcctaaaa
    10021 aaatacatta tgtatggttt ggtggtccaa aaggaaatat agaaaatata tgtataaatt
    10081 cttggaaaga aaagcttcca gaatatgaga tagtagagtg gaatgaaaaa aactttgata
    10141 tagagaaaga aataaaagga aataagtttt tggaagaatg ttataaaaga aaactttggg
    10201 cattcatatc tgattataca agaataaaag tactctatga acaaggtgga gtttatatgg
    10261 atacagatat gcaaatatta aaggatataa ctcctctttt agaaaataat aggctagtct
    10321 gtgggtatga agatgataga gagtatataa atggtgctat aataggtgta gaaaaaggac
    10381 atcctttttt aaaagactta ttggagtatt atgaaaaaga agtacttact tcttcattgt
    10441 ttacaatacc aaagataatg actcatctta tggaaaagaa ttataagaag attgatccaa
    10501 ataattatga agaaggaata cgtgtttacg acaaagaata tttttatcct tttggattta
    10561 aagaagactt tacaccagaa tgtataacag aaaacacttt tgggatacat tggtggggaa
    10621 aaagttgggc taaaaagaga aattatttct tagaaagcaa acacttaaca ggtgtaaata
    10681 agatatggaa gtgttgtaaa atatttgcaa gtaatacttt acgaagctaa aagggagaaa
    10741 gtaaaatgaa gactttaaat gatgatagaa atattatgat tagaaaatat ctttatatat
    10801 ttgttttgtt tatcatatta cttagaaagt atatgatatc atttattgac ccaaatatgg
    10861 atatagggat gattaaatca gcgttatttt attcttcaat aggaatacta atgctacttt
    10921 ttttatttga taagagaaaa agtataacag aaatggtact agttggagtt tgtgtcttac
    10981 tttatttatt aaatagggaa ggagctatat tattaatcgt tttattagct gtatcagcta
    11041 agcaaataga tgataaattt atagttaaaa attatttaat aatatcggca tgttttttaa
    11101 tggtagctat attattattt aatttatttc cttcattaat atttaatcag gaagtccact
    11161 atagatatat agaaaaaata gatatgcttg ttacaagaat ggattttgga cttggtaatc
    11221 caaatagtgt attttatcat atggttacta tatatgcagc atatatattc ttaagatata
    11281 aagattataa taaatgggat agaatacttt tatttggttc tgcatttttt gtttatcaaa
    11341 ccacatatag tagaactgga tttttcacta tattagcagg attaatattt gtagaaataa
    11401 taagatggat agatataaaa aaaataaaag gactaccaat gctcctaaag acattaccgg
    11461 taatacttac tttatttagt gtaattattg gtacagtatt tgataaaagc actttattaa
    11521 atagattatt agcaagtaga cctaagttct ggaatgtata cttggctgaa aaggggaact
    11581 ttctaaaacc ttttggaaat tcatatagtc ctgctataaa agcaactaat ccattagata
    11641 gttcttatgt atatataatt agtgtactag gagtagttgc ttgtatatta tttatgtatt
    11701 tgatgtataa aggaatagaa agctttatag aaaaagataa aaaagcatat ttagtagctt
    11761 tatttatatt tttactatac tcttttgcag aaaatttatt attagaagct tcatttagtt
    11821 ttgcagtagt attactaata aaagaagtga tattaaatga taaaagggaa atagatttgt
    11881 ggaagatgaa aagtaggagg tagtaatgtt aatatcatta ataatgccaa cattaaatag
    11941 atatgatgat atatacttac ttatggacag cttagaaaat caaacttaca aaaactttga
    12001 acttatagta gtagaccaaa atgataatag caaagttaaa gaaatagttg ataaatacat
    12061 tgataagtta gatataaagt atataaaaag ctctaaaaaa ggattgagtt ataacagaaa
    12121 tgtaggcata gacaatgctg tagggcaaat tataggtttc cctgatgatg attgtgtcta
    12181 cgaaaatgat acattagaaa aagttataaa tttctttaat aaaaataaag attataaaat
    12241 atacagttgc aaaactatgg attcaaataa agttgatgca tttaagaaaa tgtatgatgg
    12301 aacttgtgat atcacaagtt ccaacgtttt ggatactata acatccataa ctttttttat
    12361 tgattttgaa ggtaaagact atacaagatt tgatgaaaaa ttgggtgttg gtggagagtt
    12421 tggtgctggt gaagagatag attatgtttt aaacttattg agtttaggtt ttaaaggaaa
    12481 atactttgga aatgatataa tttaccatcc tgcaaaaaaa cattctaaat ctaaagaaaa
    12541 gtatcaaaaa gattataact atggaagagg ctttggagcg ctttgcaaaa aggaaatagt
    12601 ctatagaaaa aattataagt ttgcaaaagt tatggtatca aaacttgtaa gaaatatagg
    12661 aggtcttata ttgagttcca atagagatta tcatagtgcc actataaaag gaagaattaa
    12721 tggatttaga cagtacaagt tataatgagg atggtgttta tggacaaggt taaattttta
    12781 ataaatatgc ttttggcttt ttttatatat ccatttaata agcataagtt taatggtaga
    12841 gatatctggc ttataggtgg acattcaggg gatatataca atgataattc aaagtttttt
    12901 tatgagtata tgctaaaaga acataatgat gtagaaactt attgggtagt aaataaagac
    12961 agtaaagtat ttgataaaat tccaggcaaa aaattaataa gaggaagtgt agaaaactat
    13021 ctgtattatt acaattcaaa agctatagta ttttcacatg caccatcagc agatatagca
    13081 ccatacaatt ttgctgtacc tgttcttaat tattttcata aaaaaacgat aaaagtgttt
    13141 ttaaatcatg ggactataag ttttaaaaaa agaaaaccta tgaacaaaaa gttcaaaaat
    13201 attatagata acctatataa aagctataat atagttacag caagttctga atttgaaaga
    13261 aatgttatgg taaatgattg gggaatgtta gatgactcag tttatataat tggaaatgca
    13321 agatatgaca atttaccaac aaatgaagtt gcacaaactc gagatatatt atatactcct
    13381 acatggagag attggataaa atttagttca ggaaaattta cggatacaga ttattttaaa
    13441 aatataatga attttttaaa tgatgacaaa ttaaataaaa tattagatga gaaagatata
    13501 aatgtaaaaa tttatatgca tcatcttatg catgagttta tagatgatat aaaagaaaat
    13561 ataacaggaa aacgaattgt gtttttagat aaaggggtaa ctctagcaaa tgaaatcaga
    13621 aaatcagctg caaatataac tgattattca agtgtagcca tagacttttt gtatatgaat
    13681 agacctatct tgttttacca attcgatttg gatgaatata tggaaaaagt tgattcatat
    13741 atagatttaa aaagtgaaat gtttgggtct ttggcttata ataatgatga agccgtgaat
    13801 aaacttattg atattattga aaataatttt gaggttatgg ataaccaaaa aaatgaaaga
    13861 aataagtttt tcagatataa tgataataag aactgcaaga ggatatatga ttgtgtatta
    13921 agtaaaatta aataaatatt ttataaatga tgaggaaggt aatatgaaga agaatttagt
    13981 atctataatt actcccatgt ataattctga aaaatttatt gaagcaacca taaaatcagt
    14041 attaaaccaa acttatcaag aatgggaaat gttaattatt gatgattgct caacagataa
    14101 tagtcctaat atagtcaaat cttatatgca acaggatagt agaataaaat gtataaagac
    14161 tgagactaat aagggtgtct ctaatgctag aaatttagca ctaagtaagg caacaggaca
    14221 atttatagct tttttagata gtgatgacca atggaatagt agtaagttag aaaaacaagt
    14281 aaattttatg ttagaaaatg actatgtaat ttcatttact tcatatgaac tgatggatga
    14341 aaatgataaa aaattaaaca aagtaataaa agttccacct aatgtagatt atagaagatt
    14401 attaaaagga aatatcttag gatgtcttac tgttgtgatt gataagtcta aattggattt
    14461 tgaaataaga atgagtggtg taagacatga agattatgtt ttatggttat cgatattgaa
    14521 aaaagggcat attgctcatg gaataaatga agttctagca ttatatagaa aatctagcaa
    14581 ttccctaagt ggtaataaaa taaaagctgc tatgtggact tggaatatat atagaaatat
    14641 agaaaaaata cctttataca aagctatata ctactttatt aattatggaa taaacggaat
    14701 caaaaaaagt tagacagaga ggaagattta attaatgtat aaatataaat ttactgtatt
    14761 tactccgacg tataacagag ctcatttact tgaaaatttg tacaatgatt taaaagctca
    14821 aacttatgat ttcaatgatt ttgaatggtt gattgttgat gatggaagta gcgacagtac
    14881 taaagagtta gttgaaaaat ttatatcaga aaataaatta aatataagat atatatacaa
    14941 agaaaatgga ggaaaacata ctgcaataaa tctaggtgta aaaaatgcag atggagagct
    15001 atttttcatt gtagatagtg atgatggttt aattaaagat tctcttgaaa ttgctaatga
    15061 tgagtgggat tctttagaga ataaagaagg atttagtgga gttgttggac tttgtgtata
    15121 tccaagtgga gatttgatag ggacagaaat gcctgaagat aagaaaatat gtcactttgc
    15181 agacctatat tttaagtatg gcgttaaggg agataaaagc atagtatttg taactgatga
    15241 gcttataaaa tttccttttc cagaaagaaa agaagtgaga tttttaccag aaagtgtagt
    15301 atggaatgaa atgtctaaat actataaagt tatatgtgta aataaaccta tgataataag
    15361 agattatttg gatgatggtt tgactaagaa tatattaagt aaaaatgcat taagaggtag
    15421 agcattagag tttttatatt taataaatca aaatacatat ccattaagta gatatccata
    15481 tatgtggata aaaaattaca taaatttagc cagatactca ttgttgtctg atagtcatta
    15541 ttttggtgaa ttaagaaaag tatctgataa attgttgtat ttagcattat tcccattagg
    15601 atattacaaa tatattggac aaagaaaatt agtatctaag taaacttgtt ggtatagtac
    15661 tgctagttgc tatataagga gagtaaaaat atgaatgagc ctttagtatc gataattact
    15721 cctgtttata attcagaaga gtttttatca gagacgataa agtcaatcca aaatcaaact
    15781 tataaaaatt ggcagctttt attagtggat gattgttcta aggacaattc aagtgaaatt
    15841 ataaaaagtt ttcgaaaaga agatgctaga ataaaatata taaagcttga aaaaaattct
    15901 ggagctgcag tatctagaaa tgttggtatt aagaatgcgg aaggtcgatt tattgcattt
    15961 gtagatagtg atgatttatg ggatagtaga aaattagaga ttcagataga atatatgtta
    16021 aaagaaaatg taggtttttc ttttacatct tatagatata tgagacaaga tgggtcaaaa
    16081 acgaataagg ttgcaagagc tcctaagaaa attgactatg aaggattact tagaaatact
    16141 ataataggat gttctacagt ggtcattgat aaggagattg taggagagtt cagcatgcct
    16201 ttggtaagaa gaggacaaga tactgcaacc tggttacagt tacttaaaaa ggaaaaatat
    16261 gcatatggga ttcaagaaga tttagtgaat tacagattag ttggaaattc tatatcaagt
    16321 aacaagataa aagcattaaa acgaacttgg aatacttata gaaatgttga aaatttaagt
    16381 ttaccaaaaa gtctatatgt attttgtttc tatgttttta atgccattaa aaaaagagta
    16441 taagttttat atacttggag gtaaaaaaat gaaaatagct atagctggga cgggatatgt
    16501 aggtttggtt acaggtgttt gtctagcaga ggtaggcaat gaaaatgtag tctgtgttga
    16561 tgtagatgaa caaaaagtta aatcaatgaa acttggaata gcacctatat atgaagaagg
    16621 cctagaagaa cttatggtta aaaattacaa cagaggaaga atagattaca ctacagacta
    16681 taaaaaagct tatgaagatg tagatgtaat attaatagct gtaggtacac ctgagagaag
    16741 tgatggttca gccaacctag attatataaa ggcagttgca aagcaaatag ctgaaactgt
    16801 gacaaaagat actctagtag taataaaatc tacagttcca attggtacaa atgaagagat
    16861 agaagattat ataaatagaa acctaaaaaa tgatgtaaaa gtagaacttg catctaatcc
    16921 agagttttta tcacaaggaa cagcagtaaa agatactcta catgcttcaa gaatcgttat
    16981 aggtgtagaa agtgaatggg ctagagaaat attagaggaa atctataagc cttttaatca
    17041 acctataata gttacaaata gaaatagtgc tgagatgata aaatacgcat caaataactt
    17101 cttagctctt aaaatatcat ttatgaatga tattgcaaat ctttgtgaaa tagcaggagc
    17161 taacatagaa gatgtagctt taggaatgag ttatgatgat agaataggaa gtaagttttt
    17221 aaaggcggga ataggatatg gtggttcttg ctttccaaaa gacacaaaag ctcttcactg
    17281 gttatctgaa cataatggtt atgaattaaa aacagtaaaa gctgcaatag aagttaatga
    17341 aagtcaaaaa cttaaattag ttagaaaggc atctaagtta gtagagagct ttcaagatat
    17401 gaaggttgct gttttaggtt tgacttttaa gccagacact gatgatataa gagaagcacc
    17461 atctatacca aatataaaat atttgctcga aagaggggca agagttactg tatacgaccc
    17521 aattggtgta aataatacta agaaaatatt tggtgactct atactttatg ctgattctgt
    17581 agatgatgcg atagaagatg cagagatatg ttttataatg acagaatgga aagatgtatt
    17641 aaattatgat atacataagt ataaggaaaa gatgaaaaat cctttagtcc ttgatggaag
    17701 aaattgttat aaaattaaag atatggaaga gttaggaatt gagtattatt cgataggaag
    17761 gtaaatatta tgaagactgt ttgttattta gctgatgggt caaatcctca tactttaaaa
    17821 tggtgtagtt tttttaaaaa taaaggatat gacattcatg taatctcttt aaatggaggt
    17881 cacatggaag gaataaaaat ttataatttt ggttctaatg tagaagaact aaaaaatgaa
    17941 aatatttcta aaaaaacggg atacttaggt tcaataaagc agataaagaa gctagtaaat
    18001 gaaataaagc cagatatatt gcatgcacag tatgcaagta gttatggatt tataggaagt
    18061 ctacttgggt atcatccata tattatttct gtttggggta ctgatattta tgatttccct
    18121 aataatggtt ttatacaaaa acaaattata aaacataact ttagaaaagc agactatatc
    18181 ttttcaaata gtagagatat ggctaaagag gctaataaat atacagctaa acatgtagat
    18241 gtaacctttt ttggagtaga tatggatagg tttaagccta tggaagtaga aaaagaagac
    18301 gcttttgtaa taggaattat aaagagttta gaaaaaaaat atggaataga atatttaata
    18361 caagcattta agatgcttaa agatgaatat aaagataaga aaataattct taaaataggt
    18421 ggctctggaa gtcagatgga caacctaata aatttagcta aagaattggg tatagaaaat
    18481 gatgttcagt ttttaggaag aatatctcca gaaaatgtaa gcaagacttt taattctttt
    18541 gatgtaactg tatttccttc tttaagagag ggatttggag tggcagctat agaatcagaa
    18601 gcatgtgaag tacctgttat agttacaaat gtaggaggac atccagaatc tgtatgggaa
    18661 aatgaaactg gcttaattgt agaaccaaaa caaccagaag aaataaaaaa tgctataata
    18721 aaactaatgg aaaatgatga acttagactt aatatgggta agaaagggcg tcaatttgtt
    18781 agagaaaatt atgaggttaa tttaaacttt aatgatatag aaaaaattta tgattctata
    18841 tttgataaat ataaaaaata aaatgaaaaa atacagcaaa agatagatgt tgttggctat
    18901 gcttaggtgc gtagcttttg ctgtttaaag gagggcttct agttatgcga gaaagtatag
    18961 atttagagaa agaaagcttt tttaataggc tttttaaaaa atataaattt agacatttaa
    19021 cactattaat tctagatatt tgtagcgtac tagtagcgtt tcgaatgtct ttaagcttga
    19081 caggtaattt aaatgcattt accactaatg atataataat ttcag
//
LOCUS       DAELLV010000051        18957 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.051, whole genome shotgun sequence.
ACCESSION   DAELLV010000051 DAELLV010000000
VERSION     DAELLV010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18957
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.051"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(93..515)
                     /locus_tag="KPD58_001499"
     CDS             complement(93..515)
                     /locus_tag="KPD58_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HBG0265276.1"
                     /translation="MNTFKTEVLLKYSAKDIFNIFTKSARLNFPNFNEKKAVGTFVQQ
                     KNNKRFKVEITNFEKNRVYEIKTSNKRESTITRYELVHIDLENTKLIFIESESKRNIF
                     GKINSALVRVLFGKRQPEEFNKFIKKLEMELENNSVNL"
     gene            993..3896
                     /locus_tag="KPD58_001500"
     CDS             993..3896
                     /locus_tag="KPD58_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0265277.1"
                     /translation="MELRDKLVNIINTENKKDPLTDVQIAKFLSTTRENITNLRKELN
                     ISNSRQRRYPYLKSAISAILQKNKNISISEITRELMTEGFNISRRVVEELLPKESLEM
                     DVVEEDEEESNDPFETLIGNKGSLRNAVEQAKSAILYPPKGLPTLIIGESGVGKSLFS
                     RHMYEFAKQKKIVKESSNFVVFNCADYSDNPQLLLSLLFGYKKGAFTGAEYDTPGLVE
                     EADEGVLFLDEIHRLPPKGQEILFSILDRGKFRRLGETNAERKVSIMLIGATTENVET
                     NLLLTFRRRIPMLITLPPLHDRLLKEKIDLIYNIFQQECNRINAKIFVDKNVIEILAL
                     KKFSGNIGQLQNMIQVLCARAFMKFINSKDEDSESIVVVDINEVLKLKDSFKDAAFQE
                     IEYTEIRKYLKNAIFIPFNLEESSVKGSLLSDEYILPEDIYKTIEKKYYDLKSLDIED
                     IDIENILWTFILNRFADLKFNLDSNEEIFSMSDLNSFVSDSLVKLVKELMNDIVEKNM
                     NLEVNKNMFKYLAIHLEEAIKRIKLNQKIINVNLEKIKIDFSKEYEISKSFAIKIEES
                     IGIKISDDEIGFITLYIKSALKNEVKKDKVGLIIISHGRIATETVNVVKELLGVKFPV
                     AIDMPLDEKPINIYNKAVELSKIIDQGKGILFLVDIGSLTNIGQIVNKRTNISTKTID
                     RVDLLMALEATRKVSMGEEELDEIFFSLLKDRMGYNYELEKHIDKSNAIITLCLTGEG
                     TAKYISKTLEEKYDNTKCYQMSALDESLFCKIENLKETNNILAIVGTINPKIPGINFI
                     PYNKELFKNLDIYLSKSKQQDDRPIKSYERMLDEDLVIFEPDIYFKKDLLEYVCSILI
                     NKEYVEKDYLDSVLHREEMLPTYSKGAIGVPHGDSSTVNSTRFVFVKLKNPIDWGVGN
                     VNFIFMPVFQANDKEIVKNVLEILKDAEFMNNANKCFDKDAFRRIIFDKFKHL"
     gene            3929..4375
                     /locus_tag="KPD58_001501"
     CDS             3929..4375
                     /locus_tag="KPD58_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0265278.1"
                     /translation="MNVDLISLGLESNDYESVIEELGSIMCKKEYVKETYINAVLERE
                     RTLPTGLDIGDMCVAIPHTDSKHVNESNVAVGILKNPVKFNSMIDPKDRLDVELVFLL
                     AVKNPDSQVKLLKDLMSVFQNIKLLKNIKNASTKEEVAKLLDFIEI"
     gene            4498..4800
                     /locus_tag="KPD58_001502"
     CDS             4498..4800
                     /locus_tag="KPD58_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0265279.1"
                     /translation="MKQFNVLSVCGSGTVTSSMVAGKLKEVLGNKGISITTTEARPTE
                     ALNLAQSGRFDFITFTSPLQTGDYGIPTINAFACLTGIGEEAFFEEVLTVIEGIDK"
     gene            4981..6387
                     /locus_tag="KPD58_001503"
     CDS             4981..6387
                     /locus_tag="KPD58_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBG0265280.1"
                     /translation="MLEFMKSIIDTFGSAIIVPIIIFIIAKIFKVTTKKSFLSAVYAG
                     VALQGFTLILNSFTPVITPVINRMVESTGVNLPVFDVGWQATSLVAFSTSAGMIYLGL
                     GILLQTILFLIKWTDVFQPSDLWNNYSYMVWGAMVIGVTGNFPLGIACMVLLNLYSLL
                     ISELVAKRWSSYYRYPNCTIIAMHNVEASVFAVFADPIYNKLGLNKMKLNPKELEKKL
                     GFLGEPITLGLFLGMFIGILGNMTRINTMEAWGEIMKVGISTSAVMAIFPKVASMFAQ
                     AFAPITEAARKIMQKAGNREWYIAVNDAVGYGEPATLISGLILIPIMLVIAMVLPGNK
                     VLPVVDLLAIPYMVQGLVAIHNGNIPKVLVSGIIWFGLGLYVCTSTAPLFTDMATNIG
                     VAIPAGAMLITSFNILGKPLMGLVFFAFLSANPIYIGLSVVIYFVLWALFRKNKTSIL
                     DYLEKQALKNVEEEPVAV"
     gene            6731..7789
                     /locus_tag="KPD58_001504"
     CDS             6731..7789
                     /locus_tag="KPD58_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBG0265281.1"
                     /translation="MGDKMRASVLYNVGDVRYEMVDIPEITDTQVLVNVKYVGICGSD
                     LPRSMVSGLSGNTKYPLILGHEFSGEVVKIGEKVKHINVGDRVAVAPLVPCGKCDYCN
                     EGNFGLCDDYNIIGTRVNGAFAEYVRVPEEHILKLPDTLDYETAAGIEPATIAYHGIS
                     KSNIRVGDSVVVLGCGPIGQFVIQWAKVFGASKIIAVDIFDEKLELSKLLGANYILNS
                     KEVNVIKEIKKITNGGADVVIETAGSRFTQEQSLFVAKKRGNIVFVGISHTELPLSAD
                     ATECILRGELTLKGSWNSYTSPYPGRAWTATLDFMGKGDIIFKPMISHKIGLNEVGDF
                     LSKMSKREINFNKILVEI"
     gene            7888..8574
                     /gene="araD"
                     /locus_tag="KPD58_001505"
     CDS             7888..8574
                     /gene="araD"
                     /locus_tag="KPD58_001505"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBG0265282.1"
                     /translation="MLENLKEEVLKANLELPKKKLITYTWGNVSGIDREKGLVVIKPS
                     GVEYSNMTVEDMVVVDLEGNIVDGKLRPSSDTPTHLVLYKEFEELGGIVHTHSSWATI
                     WAQIGKSIPSCGTTHADYFYGSIPCTRKMTREEIRNEYEKETGNVIIEEFMGRNPIHC
                     PGIIVNDHGPFTWGKDANEAVHNAVVLEEVAKMAYYTELMSPDNIMDKVLMNKHFSRK
                     HGKNAYYGQK"
     gene            8592..9239
                     /locus_tag="KPD58_001506"
     CDS             8592..9239
                     /locus_tag="KPD58_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBG0265283.1"
                     /translation="MLDSLKGKVVEIAIKAEHYGLCKEKTGNFSIRDKDTGYILITPS
                     GVGREELKVEHICVLDLDGNVIEVEKGIKPSSEVLMHIEIYKTRENINAISHTHSLYA
                     TAFSVAKKPIIPIVYESVNYGGYVDIAPYERPGTLKLAKSVSKLLIDTDACLLERHGL
                     TTVGNDLDEALLKSRYVEEVAEIYYRSLVLNQFKELEVVDVDEFKSWKYPEEIKF"
     gene            complement(9412..10779)
                     /locus_tag="KPD58_001507"
     CDS             complement(9412..10779)
                     /locus_tag="KPD58_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HBG0265284.1"
                     /translation="MNLIDILNKVDAFIWGPPLLVLLVGTGILLTVKLGVVQITKLPR
                     ALKLIFSAENKGSGDVSSFAALCTALAATVGTGNIVGVATAIKAGGPGALFWMWIAAF
                     FGMATKYSEGVLAIKYRTKDKNGQVSGGPMYYIVNGMGEKWRPLAIFFAISGILVALL
                     GIGTFTQVNSITDAINNSFGIDPRITGVVLAVFVALVVFGGLKSISNVATKIVPFMAV
                     IYVVICGIILISFWNKIPETFMLIIKSAFTPTAATGGFLGATMSLAIRNGIARGVFSN
                     ESGLGSAPIAAAAAKTEWPAEQGLISMTGTFIDTIIICTLTGFSLVISGVWCSDLNGA
                     VMTQAAFNGAIPTFGPILLTVSLTLFAFTTILGWSYYGERCFEFLFGVKGMNGYRTVF
                     VAMVLLGAFLKLEVVWIIADIVNGLMAIPNLIALLALSPIIVSETKKYFEHINSPENQ
                     IKKNA"
     regulatory      complement(10969..11057)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            11485..13605
                     /locus_tag="KPD58_001508"
     CDS             11485..13605
                     /locus_tag="KPD58_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBG0265285.1"
                     /translation="MGKILVLAEKPSVGRDIAKVLGSKNEKNGYIEGPKYVITWALGH
                     LVTLADPESYGERYKSWSLEDLPILPKHLKTVVIKKSGKQFNTVKSQMNRNDIDEIVI
                     ATDAGREGELVARWIIEKSQVKKPIKRLWISSSTDKAIKDGFAKLKSGKEYENLYYSA
                     IARAEADWIIGINATRALTTKYNAQLSCGRVQTPTLAMLLKREEEIRNFKPKEYYGLE
                     LIATKGNSDIKFIWNDKNNNSSTFSKEKIESTLKKVKGVDIKISEVNKSYKKKYSPAL
                     YDLTELQRDANKMFGFSAKETLSIMQKLYEHHKVLTYPRTDSRYLTSDIVGTLKDRIK
                     AVNISDYSKICNRLLKSNIIANKSFVDNSKVTDHHAIIPTEQKVFMSDFSDKERKIYN
                     LVVKRFLAVLSPPFEYEQTTISATVEGEIFKAKGNKVINLGWKESYKDIEDEDEYESI
                     PNVLEKDVLKVKGLKITSGKTNPPPYLNEGTLLTAMEKNGLGTVATRADIIEKLFNSF
                     LMEKKNKDIYITSKGRQLLDLVPVDLKTPELTASWEKKLLDISKGKLSKNTFINDMKD
                     YSKDIVLEVKNSDNKFKHDNLTKNRCPECGKFMLEVNGKKGKMLICEDRECNTRKTIS
                     QVTNSRCPNCHKKLELRGEGEGRIFTCSCGYREKLSSFNKRKQEEKQKASKKDVNQYL
                     KNQNKNQETFNNPFAEALAKLKNK"
     gene            13709..14962
                     /locus_tag="KPD58_001509"
     CDS             13709..14962
                     /locus_tag="KPD58_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HBG0265286.1"
                     /translation="MDLSGIFKYYCKECENTWNNSSVELFENIETYSKDSQKKREKEL
                     DKLLNTISVHLERYPSDAVLRKMWVKKGEVFLQKTLEKENIFKLEKMDVEDRKKFLDI
                     TKQFIRDARKFDDDLPIGDIMQAMRNVWISNALQLLFGKEVYYSKANFAYSMLYPYTD
                     NYLDNTNIDKNDKILFNNWLEKRLLGEHIKSKDYHESKVSQMIDYIESVYPREKFTEV
                     YESLLLIFKSQVNSLKQHGKENHLCKEDLLSISIEKGGSSVLVDGYLISGLMTKEEIE
                     FCIGYGFLLQISDDLQDIKEDLKYNHKTIITEMSKEGTLDKVVNKLINFTIELIDSFK
                     INNKNKSVITMIKNDCLMLILFSVVYNAEFFSVGYIKEVEKFIPYTIDYSLEIEEKIK
                     EKFKNIDVLNNENEYKEMIDIICAE"
     gene            complement(15116..16177)
                     /gene="ytvI"
                     /locus_tag="KPD58_001510"
     CDS             complement(15116..16177)
                     /gene="ytvI"
                     /locus_tag="KPD58_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HBG0265287.1"
                     /translation="MDIQKQKNFIIKFTYVVLITSIVYIVLKFLLPLLMPFVISFIIA
                     SILRPIIRLITNNTNLNRTLISIVILLIFYGLCIFLLVSFGVKIFASVSDVFFRLPEI
                     YKSSIQPTLNTLFSKIDASTPNVNLALILGWDNISQSMMSLVASISTNALNAIASIAS
                     KTPAFMLKLIITLIASFFFTFDYQKIVNFILKQFPEKSQFMIINIKNSSINALLKLLK
                     AYAILLSVTFIELLIGLTILKVENAFTISVIIALVDILPVLGTGSILTPWMIISLING
                     NINLAIGLLILYIIITVVRQILEPKVVGHQIGLYPLITLMCMFVGAQLFGIAGLFGFP
                     IAATIIKNLHDNGIITAFK"
     gene            16451..17191
                     /locus_tag="KPD58_001511"
     CDS             16451..17191
                     /locus_tag="KPD58_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HBG0265288.1"
                     /translation="MKNFRDLGGNKTEDGRTVKKGLFYRSAKLSNLSENDIKILRELN
                     IKYIFDYRSDEEARKHPSTIISNIKNIRIPAMRELEESGGSFGSIEDMIDGLFEKDGA
                     FNMLNNSYYNLPINNPSYKKLVELIRDYSNLPILNHCTAGKDRTGVGSAIILMILGVS
                     RENIMKDYLKSNDFADKEIERFIEYKPKFKDIPKENLKYIFGVNEEYMKTAFRRIDEE
                     YISVEAYLYGEFNLNKEEIRKLRNQYLE"
     gene            17203..>18957
                     /locus_tag="KPD58_001512"
     CDS             17203..>18957
                     /locus_tag="KPD58_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG0265289.1"
                     /translation="MSLNITARQINIIKILLNSKDTISGIALSQEIGCSSKTIQNEIK
                     DINKQLRNGKILSIRGVGYKLEGSFDEVNLNSNLYDDVDRVEYIIRKILTLSNEEKNT
                     IKLEDLADSMYVSVSTVKNDLKEVKRMLEKYSISVKSKHKQGICVLESEDKILNCIVD
                     LSNKRDNQLSLNDFLNDDIKERKLLIKKLLLDILNENGLVLTDIEFKSVLNNILVMLS
                     RHKYNEKEFIENYIENYRSKRSSILENEQNKEIIINSIKTFCKNLKLATSIDISNDKV
                     FEEFLYKHISSLCKKIKLGINQSPIMAQDIKIKYPFAFELANIAKKTIEKDLKIEINE
                     DEVANIALHIGGAVERASYNNKDKVFKTIIVCTSGIGTSMLIKAKLENIFKEKLEILK
                     IIPSYLVEYIKVIDVDFVISTVPIEVGDVPVINISPILSEKEIRLIEKYIETGNIYLD
                     LDIKSLFDSELFFTDLVFKRKEEVIDFMASKLVQKGYIDEDMKKSYFEREKIATTEIG
                     NMVTIPHGAIGKIFDNKIAIGILKKPISWEISDVRLIIMLALDKDKILDYEVIFSKIY
                     KRVDSIAKVISICENSSFE"
BASE COUNT         7636 a         2042 c         3180 g         6099 t
ORIGIN      
        1 ttttcattaa ttattgtaac gaatagtagg actcataaaa tacttagaaa tatgttttta
       61 gatagatttt aaatatataa tctaacttat atctataaat taacactatt attctccaat
      121 tccatttcta gtttttttat aaatttatta aattcttctg gttgcctttt tccaaacaat
      181 actcttacaa gagctgaatt gatttttcca aatatattcc tcttgctctc actttcaata
      241 aaaataagtt ttgtattttc taagtcaata tgtactaatt catatctagt aatagtactt
      301 tctcttttat tgcttgtttt tatctcatat actctatttt tttcgaaatt agttatttca
      361 accttaaatc ttttattatt tttttgctgt acaaatgttc ctactgcttt tttctcatta
      421 aaattaggaa aatttagtct tgcagatttt gtaaatatat taaatatgtc ttttgcagaa
      481 tattttaata aaacttcagt tttaaatgta ttcatatcgt tttcccttct gtatataaat
      541 ttaatattta taagttaatt tttaagttaa aatagtataa tatgccatat taaatcatgt
      601 taaaactatt tatatagcta aaatcttacc tatataagta tgtatactat catataaaaa
      661 taaaatctta aaatagaaag ggttaaaaac acagtttatt ataattttta ttgttgagta
      721 gatacgtact attatatatg attgagcaag tttttcaatc ataaatttat aaagtatttt
      781 gcattaaaat ttaaacaaag aattaaatta tttaagatta tgaataacta atacatttag
      841 taaattacta aatatacaaa atgtaaatac ttatcaaaat gtataaatac ttatgacatc
      901 tagttactta cagggtttaa gtagaaaaaa atatcaaagt aagtatggca tgatagttgc
      961 ttatatattt atcaagaaag gagttattaa gcatggagtt gagagataaa cttgtaaaca
     1021 tcataaatac agaaaataaa aaagatccat taacagatgt tcaaatagca aagtttttaa
     1081 gtacaactag agagaacata actaatttga gaaaagaact taatataagt aattcaaggc
     1141 aaagaagata tccttattta aaatcagcaa tttcggcaat attacaaaag aataagaata
     1201 tttctatatc agaaattaca agggaattga tgacagaagg ctttaatata tcgagaagag
     1261 ttgtggagga acttttacct aaagaaagtt tagaaatgga tgtagtagag gaagatgaag
     1321 aagaatcgaa tgacccattt gaaactctga taggtaataa agggagtctt agaaatgctg
     1381 tagagcaggc taaatctgct atattgtatc caccaaaagg gttaccaacg ttgattattg
     1441 gggaaagtgg agttggtaag agtttatttt ctagacatat gtatgagttt gcaaagcaaa
     1501 aaaagatagt aaaagagagc tccaattttg ttgtatttaa ctgtgctgat tatagtgaca
     1561 atccacaatt attattatct ttgttatttg gatataaaaa aggtgcattt acaggtgctg
     1621 aatatgatac accaggtctt gtagaagaag ctgatgaagg agttttattt ttggatgaaa
     1681 tacatagatt gcctccaaaa ggtcaagaaa tactattttc tatcttagat aggggaaagt
     1741 ttaggagatt aggtgaaact aatgctgaaa gaaaagtaag tattatgttg attggggcaa
     1801 ctactgaaaa tgtagagact aatttattat taacattcag aagaaggata ccaatgctaa
     1861 taactttgcc accattacat gatagattac taaaagagaa aatagacctt atatacaata
     1921 tctttcaaca agagtgtaat agaataaatg caaaaatatt tgtagataaa aatgtgattg
     1981 aaatacttgc actaaaaaag tttagtggaa atataggaca attacaaaat atgattcagg
     2041 ttctttgtgc aagagctttt atgaagttta taaattccaa agatgaagat agtgaatcta
     2101 tagtagtagt agatataaat gaagttttaa agcttaaaga ttcgtttaaa gatgctgcct
     2161 ttcaagagat tgaatataca gagataagaa aatatttgaa aaatgctata tttataccat
     2221 ttaatttaga agaatcatca gtaaaaggaa gtcttttaag tgatgaatat atattaccag
     2281 aggacatata caaaacaatt gaaaagaaat attatgacct aaaatcccta gacattgaag
     2341 atatagatat agagaatatt ttatggactt ttattttaaa tagatttgct gacttaaagt
     2401 ttaatcttga tagtaatgaa gaaatttttt caatgagtga tttaaatagt tttgtaagtg
     2461 atagtttagt taaactggta aaagaactta tgaatgatat agttgaaaaa aatatgaatt
     2521 tagaagtaaa taaaaatatg ttcaaatatc tagcaataca cttagaagaa gctataaaaa
     2581 gaattaagct aaatcaaaag ataataaatg taaatttaga aaaaataaag attgactttt
     2641 caaaagaata tgaaattagt aaaagttttg caataaaaat tgaggaatca attggtatta
     2701 agatttcaga tgatgaaatt ggatttataa cgctctacat aaagtctgct ctaaaaaatg
     2761 aagtgaaaaa agataaagta gggttaataa taatttctca tggacgtata gctacagaga
     2821 ctgtaaatgt agttaaagaa cttttaggtg taaagtttcc tgtggctata gatatgcctt
     2881 tagatgaaaa accaataaat atatacaata aggcagtaga attgtcaaaa ataatagacc
     2941 aagggaaagg catattgttt ttggtggata ttggttcact tacaaacata ggtcaaatag
     3001 taaataagag aaccaatata agtactaaaa ctattgatag agtagacctc ctaatggcac
     3061 tagaagcaac tagaaaagtt tctatgggag aggaagaact tgatgaaata tttttttctt
     3121 tgctaaaaga taggatggga tataactatg aattagaaaa acacatagat aaatcaaatg
     3181 caattataac attatgtctt actggagaag gaactgctaa gtatataagt aaaacgttgg
     3241 aagaaaagta tgataacact aaatgttatc aaatgagtgc attggatgaa agtttatttt
     3301 gtaaaattga aaatctaaaa gagacaaaca atatattagc tattgttggg acaataaacc
     3361 ctaagatacc aggtataaat tttataccct ataataaaga acttttcaaa aatttggata
     3421 tctatctatc aaaatcaaaa caacaagatg acagaccaat taaatcgtat gaaagaatgc
     3481 ttgatgagga tttagtgatt tttgaaccag atatatattt taaaaaggat ttattagaat
     3541 atgtatgttc aattcttata aataaagaat atgtagaaaa agattactta gattctgttt
     3601 tacatagaga agaaatgctt ccaacatatt ctaaaggagc tataggtgtg cctcatggag
     3661 attcttctac tgtaaatagt acaaggtttg tttttgttaa actaaagaat cctattgatt
     3721 ggggtgtagg taatgtaaat ttcatattta tgccagtttt tcaggctaat gataaagaga
     3781 ttgttaaaaa tgttcttgag atactaaaag atgcagaatt tatgaacaat gccaataagt
     3841 gttttgataa agatgctttt agaaggatta tatttgataa atttaaacat ctgtagccat
     3901 agcaaaaaaa ttgagaggag ttagagtaat gaatgtagat ttaatatcat tgggtttaga
     3961 atctaatgat tatgaaagtg ttattgaaga attaggaagt ataatgtgta aaaaagaata
     4021 tgtaaaagaa acatacataa atgctgtctt ggaaagagaa aggacattac caacaggtct
     4081 agatattgga gacatgtgtg ttgctattcc acatacagat tcaaagcatg taaatgagtc
     4141 taatgtagct gtagggatat tgaaaaatcc tgtaaagttt aattcaatga tagacccaaa
     4201 agatagactt gatgtagaac ttgtattttt attagcagta aagaacccag actcacaagt
     4261 aaaactgtta aaagatttaa tgtcagtttt tcaaaatata aagttattaa aaaatataaa
     4321 aaacgcatca acaaaagaag aagtagctaa attattggat tttatagaaa tttaatgtag
     4381 gagaatttta taaataaaaa attaaaattt aaacaatcaa agtaagtatt atttttttaa
     4441 taggtttaat cttatataaa ataaaaattt agagtgaaaa attaggagga aattattatg
     4501 aaacaattta atgtattatc agtttgtggt tcagggacag ttacatcatc tatggtagca
     4561 ggtaaattaa aagaagtact aggtaataag gggatttcta ttactacaac agaagctaga
     4621 ccaacagaag cactaaattt agcacaatca ggaagatttg actttataac atttacgagt
     4681 ccacttcaaa caggcgatta tggaatacca actataaatg catttgcttg cctaactggt
     4741 ataggggaag aggcattctt tgaagaagtt ttgactgtta ttgaagggat agataagtaa
     4801 gttttggaga cgtgaattta tagtatttaa atcaatgatt tagatactat aataagctaa
     4861 tattaactaa atatttgtgg aaaaaacatt ttatatgttg tcataaatta aaaaattata
     4921 aaaatagtca cttaagtatt aaattaagct tataaatatt tataagtagg agggaaaaag
     4981 atgttagaat ttatgaaatc tataatcgat acatttggtt ctgcaattat agttccaatt
     5041 ataatattca taatcgcaaa gatatttaaa gtaactacta agaaatcgtt tctttctgct
     5101 gtgtatgcag gagttgcttt acaaggtttt acactgattt taaattcatt tacaccagta
     5161 ataacaccag ttatcaatag gatggttgag agtacaggag taaacctacc agtttttgat
     5221 gttggttggc aagcaacatc attggttgca ttttcaacaa gtgcaggtat gatatattta
     5281 ggtcttggta tattattaca aacaatttta tttttgataa aatggacaga tgtgtttcaa
     5341 ccatcagatt tatggaataa ctattcttat atggtatggg gagcaatggt tataggagta
     5401 acaggaaatt ttcctttagg tatagcatgt atggttttat taaacctata tagtttatta
     5461 atatcagagt tagttgcaaa gagatggtct agttactata gatatcctaa ttgtacaata
     5521 atagctatgc ataatgttga agcttctgtg tttgcagtat ttgcagaccc tatatacaat
     5581 aaattgggat taaataaaat gaaattaaat ccaaaagaat tagagaagaa attagggttt
     5641 ttaggtgaac caattacttt aggtcttttt ttaggtatgt ttataggtat attaggtaat
     5701 atgacgagaa taaatactat ggaagcttgg ggagaaataa tgaaagtagg tatatctaca
     5761 agtgcagtga tggcaatttt ccctaaagta gcaagtatgt ttgctcaagc atttgcacct
     5821 ataacagaag cagctagaaa gattatgcaa aaagctggaa accgtgagtg gtacatagca
     5881 gtaaatgatg ctgttggata tggtgaacct gcaactttaa tatcaggtct tatattaatt
     5941 ccaataatgc ttgtaatagc tatggtactt cctggtaata aggtattacc agttgtggat
     6001 ttattggcaa ttccatatat ggttcaaggt ttagttgcca tacataatgg taatatacca
     6061 aaagtattag tttcaggtat aatttggttt ggattaggat tatatgtttg tacatctaca
     6121 gcaccattat ttacagatat ggctacaaat ataggtgtag ctatacctgc aggagcaatg
     6181 ttaataacaa gttttaatat actaggaaaa cctttgatgg gattagtttt ctttgcattt
     6241 ctaagtgcaa atccaattta tataggtctt tcagttgtaa tttactttgt tctatgggca
     6301 ttgtttagaa agaacaaaac aagtattctt gattatttag aaaaacaagc acttaaaaat
     6361 gtagaagaag agcctgtagc agtttaagtt tataaaaatg aaataaaaat aattaaagga
     6421 gtttataatt gattttatat aagtatcaga taatacctga gaaatattta aatgaaatat
     6481 agacttcttt ttatttgata atatttgtaa ttataatgca ttgtatcttt atagttgagt
     6541 tttctgtata acctgctatt taaaacatac tcttttatat ttatacagta atgtggttta
     6601 attttataaa atgtaatctc tactggtaaa agactttgta tataaggcat ttgcctgaat
     6661 aaaccattta tcttttgtat ggcatagata ttgctatata gaagtatata gtatgaaagg
     6721 agatatttat atgggagata aaatgagagc atcagtattg tataatgtag gtgatgtcag
     6781 atatgaaatg gtagatatac cagaaataac agatacgcaa gtgcttgtaa atgtaaaata
     6841 tgttgggata tgtggttcag atttacctag gtctatggta agtggtttga gtggaaatac
     6901 aaaatatcca ttgattttag ggcatgaatt ttctggtgaa gttgttaaaa ttggagaaaa
     6961 agtaaagcat attaatgtag gagatagagt agctgttgca ccattagtac cttgtggaaa
     7021 atgtgattat tgtaatgaag gtaattttgg cttgtgtgat gattacaata taattggaac
     7081 aagagtaaat ggagcgtttg cagaatatgt aagagttcca gaagaacata ttttaaaact
     7141 accagataca ttggattatg aaactgctgc cggaattgaa ccagcaacta tagcttatca
     7201 tgggatttca aagtctaata ttagagtagg agatagtgta gttgtgttag gatgtggtcc
     7261 aataggtcag tttgttatac aatgggcaaa ggtgtttggt gcatctaaga ttatagcagt
     7321 tgatattttt gatgaaaaat tagaactttc taaacttcta ggagcaaact atatacttaa
     7381 ctcaaaagaa gtaaatgtaa tcaaagaaat aaaaaaaata acaaatggtg gtgctgatgt
     7441 tgtaatagaa actgctggta gccgatttac acaagaacaa tctctatttg tagcaaaaaa
     7501 gagaggaaat atagtgtttg taggtatatc acacacagaa ttaccactta gtgcagacgc
     7561 tactgagtgt atattacgtg gagaacttac cttgaaaggt tcttggaatt catatacaag
     7621 cccttatcct ggaagagcat ggactgcaac attggatttt atgggaaaag gtgacattat
     7681 ttttaaaccc atgatttcac ataaaatagg tcttaatgaa gtgggtgact ttttaagtaa
     7741 aatgagcaag agagaaatta atttcaataa aatcctagta gaaatataaa aatttaataa
     7801 aaaattctaa aagcaattaa attttataac tatgacatta aataaaataa aaataatttt
     7861 cttaaataaa atataggagg aagaaaagtg ttagaaaatt taaaagaaga agtattaaaa
     7921 gcaaacttag aattacctaa gaaaaagcta attacttata cttggggaaa tgtaagtggg
     7981 atagatagag aaaaaggatt agtggtgata aaaccaagtg gtgtagagta tagtaatatg
     8041 actgtagaag atatggttgt agttgattta gagggaaata tagttgatgg caaattaaga
     8101 ccttcttcag atactccgac tcatctagtt ttatataaag agtttgaaga attgggggga
     8161 atagtacata cacattcaag ttgggcaaca atttgggctc aaataggaaa atctatacca
     8221 tcttgtggta ctactcatgc agattatttt tatggaagta taccatgtac cagaaaaatg
     8281 actagagaag aaattcgcaa tgaatacgaa aaggaaactg gaaatgtaat cattgaagaa
     8341 tttatgggaa gaaatccaat tcattgtcca ggaataattg taaatgacca cggacctttt
     8401 acatggggaa aagatgcaaa tgaggctgta cacaatgcag tagttttaga agaagtcgca
     8461 aagatggctt attatacaga gttaatgagt cctgataata ttatggataa ggttcttatg
     8521 aataagcatt tttcaagaaa acatggaaaa aatgcatact atggtcaaaa atgattatct
     8581 ggaggtgaat tatgctagac tctttaaaag gtaaagttgt ggaaatagca attaaagcag
     8641 agcattatgg tttatgtaaa gaaaagactg gtaattttag tattagagat aaggatactg
     8701 gatatatact tataacccca tcaggtgtag gaagagaaga attaaaagtt gaacatattt
     8761 gtgtattgga tttggatgga aatgtaatag aagttgagaa aggtattaag ccaagtagtg
     8821 aggtattgat gcatattgaa atatacaaaa caagagaaaa tataaatgcc atatcacata
     8881 ctcattcttt atatgcaaca gcattttcag tagcaaagaa accaattata ccaattgtat
     8941 atgaatcagt taattatggc ggttatgtag atattgctcc atacgaaaga ccaggaactt
     9001 taaaattagc taagagtgtt tcaaaattac tgatagatac agatgcatgc ttattagaaa
     9061 gacatgggtt gacaactgta ggtaatgatt tagacgaagc attattaaaa tcaagatatg
     9121 tggaagaggt agcagaaata tactataggt ctttagtatt aaatcaattt aaagaacttg
     9181 aagtagtaga tgttgatgag tttaaatctt ggaaataccc agaagaaata aagttttaat
     9241 ctaaataatt ttcattgaag tggttaatta aaatatatgt attatttata tcaattacta
     9301 tgttttatta tgttaattac cgtattttgc aaatatattt attacatttt ttcaaaaaaa
     9361 tatagaatcc gaaagccata taaaattgac ttgggattct atacttttta tttatgcatt
     9421 tttctttatc tgattttctg gagaatttat atgttcaaaa tattttttag tttcagatac
     9481 tattataggt gataatgcaa gtaaagctat aaggttaggt attgccataa gcccatttac
     9541 aatatcagca attatccaaa caacttctaa ctttaagaaa gcacctaata acaccatagc
     9601 cacaaataca gttctatacc cattcatgcc ttttacacca aacagaaatt caaaacatct
     9661 ttctccataa taactccatc ccaaaattgt agtaaatgca aataatgtta aactaactgt
     9721 aagtaatatt ggtccaaatg taggaattgc tccgttaaaa gctgcttgtg tcataacagc
     9781 tccatttagg tctgaacacc acactcctga aataacaaga gagaatccag taagagtaca
     9841 gattataata gtatctataa aagtacctgt catagatata agaccttgtt ccgctggcca
     9901 ttcagtttta gcagcagcag cagctattgg agcactacct agaccagact catttgaaaa
     9961 tacaccacga gcgataccat ttctaattgc tagtgacata gttgctccta aaaaaccacc
    10021 agtagcagca gtaggtgtaa atgcactttt aattattaac ataaaagttt cagggatttt
    10081 attccaaaaa gatattaaaa tgattccaca gattacaaca tatataactg ccataaaagg
    10141 tactatttta gtagctacat tagatatact tttaagtcct ccaaatacaa ctaaagctac
    10201 aaatactgct aatacaaccc ctgtaattct agggtctatc ccgaaactat tatttattgc
    10261 atctgttatt gaatttactt gagtaaaagt accgattcct aaaagtgcaa ctagtatacc
    10321 acttatagcg aaaaatattg caagaggtct ccatttttct cccataccat ttactatgta
    10381 atacattggc ccaccagaca cttgaccatt tttatcttta gttctgtatt ttattgcaag
    10441 aacaccttct gaatacttag tagccattcc aaagaatgct gctatccaca tccaaaaaag
    10501 tgcaccaggt ccaccagctt tgatagctgt agcaacacca acaatattac cagtaccaac
    10561 tgtagcagct agagcagtac ataaggctgc aaaacttgaa acatcaccac tacctttgtt
    10621 ttcagcagag aatattaact taagagctct tggcagttta gttatttgaa caactccaag
    10681 cttaacagta agtagtatac cagtaccaac taatagtacc agtaggggtg gtccccaaat
    10741 aaaagcatca actttgttta aaatatcaat tagattcata aaaacactcc ctcatttttt
    10801 ttgctttaca taatcaatag aatttttaat gaagtaattg acaggaaata agtataattt
    10861 attaataatt tctagtgtaa taaaaaaagc taaatctaaa aaagattcag ctctggatgt
    10921 acactaatta gcaaccaaaa taaaagtatt agtaaatact gtttttggta gataatccct
    10981 gtccttttgc ctgagagatt gagcataaaa gccttgcccc ttcggcgccc ataatgggtc
    11041 tctccagagt tccgtaaatt ttacagtcct acttttaaaa gtacctgaga gtgttactcc
    11101 ttcggtgtgc tatatagcaa ctcttctata aaatcttaac cggatattga tttgtcatag
    11161 cttattgtat taataatatt aatataagtt tttatgtttt gtcaactaaa aaattaaaaa
    11221 acaaagaaaa aatataaaaa tgtaagccta tttacgaatt ataaacttag aaagatgagt
    11281 aatatacgtg aaaatgaatt ttgtaataca catacaaatg aagtgtaaaa taagttttaa
    11341 ataaaaaaat aataaaatct taaataatta attagatatg attaaattat ggtaatatta
    11401 tagttacgat attaagaaaa gttctattta gttagatttt aagcgtaata tgagattatg
    11461 tatagagaaa ggatgtattt ttagatggga aaaatattag tgctagcaga aaaaccttct
    11521 gttggaagag atatagcaaa agttttagga tcaaaaaatg aaaaaaatgg ttatatagag
    11581 gggccaaagt atgtgataac ttgggcacta ggtcatttgg ttacacttgc tgaccccgaa
    11641 agttatggag aaagatataa atcatggagc ttagaagacc tgccaatact tcccaagcac
    11701 cttaagactg ttgtaataaa aaagagtgga aagcagttta atacagttaa atcgcaaatg
    11761 aatagaaatg atatagatga aatagttata gcaacagatg caggtcgtga aggagaactt
    11821 gtagcaaggt ggataataga aaaatcacag gtaaaaaaac ctataaaacg tctttggatt
    11881 tcttcaagta cagacaaagc aataaaagac ggatttgcaa aattaaaaag tggaaaagaa
    11941 tatgaaaatt tgtattactc agcaatagca cgagcagaag ctgattggat tattggaata
    12001 aatgcaacca gagctttgac aacaaagtac aatgcacagc tttcttgtgg aagagtacaa
    12061 actcctactc ttgcaatgtt gcttaaaaga gaagaagaaa taagaaattt caaacctaaa
    12121 gagtattatg gattagaatt aattgctaca aagggaaatt cagatataaa atttatttgg
    12181 aatgataaaa acaataattc ttcaactttt agtaaagaaa agatagaatc cacattaaaa
    12241 aaagtaaaag gtgtagacat aaaaatatct gaagtaaaca agtcatataa gaaaaaatat
    12301 tcacctgcac tctatgattt aacagaatta caaagagatg caaataaaat gtttggattt
    12361 tcagcaaaag aaactttatc tataatgcaa aaactgtatg aacatcataa agtacttact
    12421 tatcctagaa ctgattctag atatttaaca tcagatattg ttggaacatt aaaagataga
    12481 ataaaagctg taaatattag tgattattca aagatttgta atagattatt aaaaagcaat
    12541 attatagcta ataaatcgtt tgttgacaat tcaaaggtaa cagaccacca tgcaataata
    12601 ccaacagaac agaaggtatt catgagtgat tttagtgata aagagcgaaa aatatataat
    12661 cttgtagtaa aaagattttt agcagtacta tctcctcctt ttgaatatga gcagacaact
    12721 ataagtgcaa ctgtagaagg agaaatattt aaggctaagg gaaacaaagt aataaattta
    12781 ggttggaaag aaagttataa agatattgaa gatgaagatg agtatgaaag tataccaaat
    12841 gttttagaaa aagatgtact taaggtcaag ggattaaaga taacttcagg aaaaacaaat
    12901 ccaccaccat acttaaatga gggaacatta ttgactgcta tggaaaagaa tggtctaggg
    12961 acagttgcta caagagcaga tataatagaa aaattattca attcattttt aatggaaaag
    13021 aaaaataaag atatatatat aacatcaaaa ggaagacaat tattagattt agttccagta
    13081 gacttaaaga caccagaact tacagcttca tgggaaaaga aattattgga tatttcaaag
    13141 ggaaaactta gtaaaaatac attcataaat gacatgaaag attattcaaa agatattgta
    13201 ctagaagtaa aaaatagtga taataaattt aaacatgata acttaactaa aaatagatgt
    13261 ccagaatgtg gaaaatttat gttagaggta aatggtaaaa aaggtaagat gttgatttgt
    13321 gaagatagag aatgtaatac tagaaaaact atatcacaag taaccaattc aagatgtcct
    13381 aactgtcata aaaaattaga acttcgtgga gaaggtgagg ggagaatctt cacttgtagt
    13441 tgcggataca gagaaaagtt gtcttctttc aacaagagaa agcaagaaga aaaacagaag
    13501 gcatcaaaaa aagatgttaa tcaatattta aaaaatcaaa ataaaaatca agagacattt
    13561 aataatccat ttgcagaagc attagctaag ctaaaaaaca aataatttta aagttattat
    13621 tatacacaat tataacttta actgtatatt agttatatgt actgatataa tatagtgtat
    13681 agtttaggca tgaaagtagg gctgttaaat ggatttaagt ggcatattta aatactattg
    13741 taaagagtgt gaaaatacat ggaataattc gagtgttgaa ttatttgaga atatagaaac
    13801 gtatagtaaa gattcacaaa aaaagaggga aaaagaatta gataaattgc taaatacaat
    13861 atcagttcat ttagagaggt atccaagtga tgctgtattg agaaaaatgt gggtaaaaaa
    13921 gggcgaggtt ttcttacaaa agacattgga aaaagaaaat atttttaagt tagaaaaaat
    13981 ggatgtagag gatagaaaaa aatttttaga tataacaaaa cagtttatta gagatgctag
    14041 aaaatttgat gatgatttac ctataggtga tattatgcaa gctatgagga atgtatggat
    14101 ttcaaatgca ttacaattat tatttggtaa agaagtatat tattcaaaag ctaactttgc
    14161 atatagtatg ttatatccat atactgataa ttatttagac aatacaaata tagataaaaa
    14221 tgataagatt ttatttaata actggttaga aaaaaggctc ctgggagaac acattaaatc
    14281 taaggattat catgaaagta aagtatctca aatgatagat tatattgaaa gtgtataccc
    14341 tagagaaaag tttacagaag tttatgaatc gttattatta atatttaaaa gtcaagtaaa
    14401 tagtttaaaa caacatggta aggaaaatca tttgtgtaaa gaagatttat tatccatttc
    14461 tattgaaaaa ggaggttcat ccgttttagt agatggatat ttaataagtg gattgatgac
    14521 aaaggaagaa atagagtttt gtataggata tggattttta ttacaaatat ctgatgattt
    14581 acaagatata aaagaagatt taaaatacaa ccataaaact attattacag agatgtcaaa
    14641 agagggtact ttagataaag ttgtaaataa actaataaat tttactattg agttaataga
    14701 tagttttaaa attaataata aaaataaatc tgtaataact atgataaaga atgattgctt
    14761 aatgttaatt ttattttctg tagtttataa tgctgaattt ttttctgtag gatatataaa
    14821 agaagtagag aaatttattc catatacaat agattattca ttagagattg aagaaaaaat
    14881 aaaagaaaaa tttaaaaata tagatgtttt aaataatgaa aatgaatata aagaaatgat
    14941 tgatattatt tgtgcagagt agaaaaaaag ggagtgtctt agaatagact ttttagttta
    15001 tgaggcactc tttttgtata aaatcaaatg tattttcatc attttaacaa ttaaaaagaa
    15061 cttatcccta tttgagaaag ccctttttaa tttaaataaa aatgtcaagg ttaaactatt
    15121 taaatgcagt aataatccca ttatcatgta agttttttat gattgtagct gcgataggga
    15181 atccaaataa tccagctatt ccaaatagtt gagccccaac aaacatacac attaatgtta
    15241 ttaaaggata taggccaatt tggtgtccta ctacctttgg ttctaatatt tgtctaacaa
    15301 cggttataat tatgtatagt attaaaagtc ctattgctaa attaatattt ccattaatga
    15361 gtgagataat catccaaggt gtcaaaatac ttcctgtacc caagactggc aaaatatcaa
    15421 caagtgctat gatgacagat atagtaaatg cattttcaac ctttaggata gtaagtccaa
    15481 taagtagttc tatgaatgtc actgatagta gtattgcata ggcttttagt agttttaaaa
    15541 gagcattaat actgctattt tttatattta ttatcataaa ttgacttttt tctggaaatt
    15601 gttttaagat aaagtttact attttttgat agtcaaaagt aaagaaaaaa gaagcaatta
    15661 aagtaataat aagttttaac ataaatgcag gtgttttact tgcaatacta gcaatagcat
    15721 tgagtgcatt tgttgaaata gaagctacta aagacatcat agattgactt atattatccc
    15781 aaccaagtat taatgctagg ttaacattag gtgtactagc atctatcttt gaaaataaag
    15841 tatttagcgt tggttgtata cttgatttat atatttcagg aagtctaaaa aatacatcac
    15901 ttacacttgc aaatatcttc acaccaaagc taactaataa aaatatacac aacccataaa
    15961 aaattaaaag tattactata gaaataagtg tacgatttaa atttgtatta tttgtaataa
    16021 gtcttataat aggtcttaag atggatgcaa tgataaaact gattacaaaa ggcattaata
    16081 atggcaataa gaattttaat acaatatata caatacttgt tattaaaact acatatgtaa
    16141 atttaattat aaaattcttt tgcttttgaa tatccataat ataaaacctc caatgtattt
    16201 gatattattc taaatatggt cttttttaca gaaaatatac tactgtatat tgattataaa
    16261 ctataaagct tatgtaaagt aaataaatta tgtaaaattt aggttaattt tgtataaaga
    16321 gatactttta tattttacaa ttaataacca aatactaaaa aatattttaa ataaagaact
    16381 taaaattgtg ataaaattaa tttatagata ttaaaaaaag tataatatat ggtttttaag
    16441 aggtgctaag atgaaaaatt ttagagattt aggtgggaac aaaactgagg atggcagaac
    16501 tgtaaagaag ggattatttt ataggtctgc aaaattatca aatctaagtg aaaatgatat
    16561 aaaaatttta agagagttaa atattaaata tatatttgat tatagaagtg atgaagaagc
    16621 tcgtaaacat ccatcaacaa taatatcaaa tataaaaaat ataagaatac cagcaatgag
    16681 agagctagaa gaatcaggag gaagttttgg ttctattgaa gatatgattg atggattgtt
    16741 tgaaaaagat ggtgcattta atatgttaaa taatagttac tataatttgc ctataaataa
    16801 tccgtcatat aaaaaattag tagaacttat aagagactat tctaatttac caatattaaa
    16861 tcattgtaca gctggtaagg atagaactgg tgtggggagt gctataattc ttatgatttt
    16921 aggggtatcg agagagaata taatgaaaga ttatctaaaa agtaatgatt ttgcagataa
    16981 agagatagaa agatttatag aatataagcc taaatttaag gacattccaa aagaaaattt
    17041 gaaatatata tttggtgtaa atgaagagta tatgaaaact gcatttagaa gaattgatga
    17101 agaatacatt agtgtagaag catatttata tggagaattt aatttaaata aagaagaaat
    17161 aagaaaatta agaaatcaat atctagagta aaaggtgagt ttatgagttt aaatataaca
    17221 gcaagacaga taaatataat aaaaatattg ttaaattcaa aagatacaat tagtggtatt
    17281 gccttaagtc aagagatagg atgttcttca aaaactatac aaaatgaaat aaaggatatt
    17341 aataaacaac ttagaaatgg taaaattttg tcaataagag gagttggtta taaactagaa
    17401 ggaagttttg atgaggtaaa tctaaatagc aatctctatg atgatgtaga tagagtagaa
    17461 tatataatca gaaaaatatt aaccttaagt aatgaagaaa aaaatactat aaaacttgaa
    17521 gatttagccg attctatgta tgtaagtgtt tcaacagtaa aaaatgattt aaaagaagta
    17581 aaaagaatgt tggaaaagta ttcaatatca gttaaatcta agcataaaca aggtatatgt
    17641 gttttggaaa gtgaagataa gatattaaat tgtatagttg acttaagcaa taagagagat
    17701 aatcaattga gtttaaatga ttttttaaat gatgatataa aggaaagaaa actattaata
    17761 aaaaaattat tattagacat attaaatgaa aatggattag tattaactga tatagaattt
    17821 aagagtgtgt taaataatat attagttatg ttaagcagac ataagtataa tgaaaaagaa
    17881 tttatagaga attatataga aaattatagg agcaaaagaa gctcaatttt agaaaatgaa
    17941 caaaacaaag aaattataat aaattcaata aaaacatttt gtaaaaattt aaaattagct
    18001 acatcaatag atataagtaa tgataaggta tttgaagagt ttttatataa gcatattagt
    18061 agtttatgta aaaaaataaa gttaggtata aatcaatcac ctataatggc tcaagatata
    18121 aaaataaaat atccatttgc ttttgaacta gctaatatag ctaaaaagac aatagaaaaa
    18181 gatttgaaga tagaaattaa tgaagatgaa gttgcaaaca tagctctaca tataggagga
    18241 gcagttgaaa gagcatctta taataataaa gataaggttt tcaaaactat aatagtttgt
    18301 acttcaggca taggcacatc aatgttaata aaagctaaat tagagaatat atttaaggaa
    18361 aaattagaaa tactaaagat tattccttca tacttagttg aatatataaa agttatagat
    18421 gttgattttg taatatctac tgttcctatt gaagtaggag atgtgccagt aataaatata
    18481 tctccaattt taagtgagaa agaaattaga ttaattgaaa aatatataga aacaggaaat
    18541 atatacctag atttagatat aaaaagcctg tttgatagtg aattgttttt tactgatttg
    18601 gtgtttaaaa ggaaagaaga agttatagat tttatggcaa gtaaacttgt acaaaagggc
    18661 tatatagatg aggatatgaa aaaatcttac tttgagagag aaaaaatagc aacaacagaa
    18721 atagggaata tggtaactat accacatgga gctattggaa aaatttttga taataaaata
    18781 gcaattggta tattaaagaa gcctatttct tgggaaatat cagatgttcg tttaataata
    18841 atgttagccc ttgataaaga taaaatatta gattatgaag taatattttc aaaaatatat
    18901 aaaagagtag attctatagc aaaagttata agtatatgtg aaaatagtag ttttgaa
//
LOCUS       DAELLV010000052        18924 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.052, whole genome shotgun sequence.
ACCESSION   DAELLV010000052 DAELLV010000000
VERSION     DAELLV010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18924
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.052"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            167..823
                     /locus_tag="KPD58_001513"
     CDS             167..823
                     /locus_tag="KPD58_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265290.1"
                     /translation="MKKGDFIWSGVLICIIAVLAIPSSREVFMEATGAHPYIGGFLKF
                     AVLATMGDLLGARILYKDWKIPVGVFYRAIVWGIIGVVTTYGMSIYSSGVGQAQVDGM
                     LPFEGIAFANALFKSATMNILMSPTVFLFHKIMDCFIDKKYEVGKGIKVKLKDVVNIV
                     DWNAYLGFSVLKTIPFFWIPCHTIVFLFPAEYRVIASAFASIALGLILALANKSKVAV
                     "
     gene            complement(1259..2530)
                     /gene="sleC"
                     /locus_tag="KPD58_001514"
     CDS             complement(1259..2530)
                     /gene="sleC"
                     /locus_tag="KPD58_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic protein SleC"
                     /protein_id="HBG0265291.1"
                     /translation="MQDGFLTVSIIDATNNRPIQNAVVNIYSMSNGSQSSSTLYQNLR
                     SNESGQVTGLVLPAPDVDYSLQPSDVRPYSQYIVEAIADGYETVVIEGTQLLATIEAR
                     QGVPMSPRTRSKRSFSRQSELIFDIGEHTLYGTYPPKIPESNLKPLPPPTGFVVLDNP
                     VVPEFIVVHDGLPEDSSAPNYWIPFKEYIKNIASSEIYSTWPEQTIYANVIAIISFTL
                     NRVFTEWYRNKGYNFTITSTTAYDHKFINNRNLFEPINVVVDAIFNTFIKRPPTSRQP
                     LLAQYCDGQKSQCPDQMTQWGSKDLGDQGYDYESILRYFYGDEIVFERAPIVSGVPVS
                     FPGTTLQVGSSGQYVRTIQNQLNAISNSYPAVPKVIEDGIYGTDTENAVKIFQGIFGL
                     PQSGVVDFKTWYEISRVYVATTRIASLNPLI"
     gene            2775..3491
                     /locus_tag="KPD58_001515"
     CDS             2775..3491
                     /locus_tag="KPD58_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HBG0265292.1"
                     /translation="MKLDLFLLAIVPILIGMFWIRSKDRYCREPLIHLIKFFLIGAFL
                     SVIIILLENLLMKFNVFEGYSELIYVSFVVAGLVEEGVKALILIPALIKEKHFTEKLD
                     GIIYSVFLALGFATIENMVYIFSESRNLALQVGINRAVISIPAHVMFAITMGYYISKY
                     KFEGNKNKRREYLFMAVLIPILLHGVFDFILMIEYRWAIILLIVYVIILWKINLDKLE
                     KYMNHSKKVFFGNLRKKKKK"
     gene            3570..4880
                     /locus_tag="KPD58_001516"
     CDS             3570..4880
                     /locus_tag="KPD58_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HBG0265293.1"
                     /translation="MTELPKEFLEDIEEILKDEYDDFIKSYEESKTTGLRINTLKIDK
                     KKLFDLELFDLNQIPWAEEGFYYDDKIDRPGRNPLHEAGAYYLQEPSAMSVIPKADIK
                     EGEKVLDMCAAPGGKSTYILSKLNDTGLLVSNEINASRIKALGENLERFGARNCIITN
                     TDSNSLRKTFNGYFDKVIIDAPCSGQGMFRKDEVAISDWSYAKVVECQSIQKEIIRDG
                     YKMLKKDGILIYSTCTFSREENEHVINEFISEYPHAELIEMERLWPHKIKGEGHFVAK
                     IKKLEEEECNVKELKIKNLDKEIKDYREFEKKFLKVDFYSNKKNKFYLRGENLYLLPE
                     IYPDTKKLKVLRYGLHLGVLKKNRFEPSHALSHYLKVEDVKNVENFSVQSESILKYLK
                     GDVVNSNDSRGWVLVSVEGIPLGWGKESSGVIKNHYPKGLRKVF"
     gene            4980..5516
                     /gene="lepB"
                     /locus_tag="KPD58_001517"
     CDS             4980..5516
                     /gene="lepB"
                     /locus_tag="KPD58_001517"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBG0265294.1"
                     /translation="MGEAVKKEVVEWIKVIVIALVLAFAITRFIVPTIVKGESMYPTL
                     VERDYLIVNRIAYKVGEPKYKDIIVFKTDLTEENGKKKDLVKRVIGVPGDHVKIQDSK
                     VYVNDKLLDETSYIHNNRTDGDIDIVVPEGKLFAMGDNREKSLDSRYDEVGLVDEHTI
                     LGKVLVRLYPFSKIGTID"
     gene            5682..6218
                     /gene="lepB"
                     /locus_tag="KPD58_001518"
     CDS             5682..6218
                     /gene="lepB"
                     /locus_tag="KPD58_001518"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBG0265295.1"
                     /translation="MNETIKEEIVEWIKIIITALFFAFIITRFIKPTLVNGESMYPTL
                     KSHDYLVANRMTYKLSEPKCGDIMIFKTDLLQENGRKKELVKRVIGVPGDHLKIKDSK
                     VYINGKLLNEVSYIHDNYTEGDIDMVIPKGKVFAMGDNREVSLDSRYKEVGLVDEENI
                     KGKVILRVFPFTDIGIFE"
     gene            6228..6983
                     /locus_tag="KPD58_001519"
     CDS             6228..6983
                     /locus_tag="KPD58_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HBG0265296.1"
                     /translation="MAVGIASLLFNIEEALNICESIKQITHLEIGIDNISECSELCKY
                     KERISKLGLSIGIHLPMELNTCENIEYIRNSWINFIEKIEFELKGFDLRYFNLHLGYV
                     MTNRVRKNRDKYLGNSVDFLDKLNTNSYVCIENTYSKGGDFSNIGNISYDFEYIFKRI
                     KNSKICFCYDTGHCLIDEDAYVKNLKDKIRLIHLSDNDGINDTHVGIGRGILSEEGIK
                     EVLTLDAEYLVLEINYEDIEDTISKLNNIVGEG"
     gene            7057..8724
                     /locus_tag="KPD58_001520"
     CDS             7057..8724
                     /locus_tag="KPD58_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HBG0265297.1"
                     /translation="MKKINLKIEGKDKEYSLEENSPGIRLGDIAKEFCDEHKGYITLA
                     VVDNKLKELNCRVKKDCEINFLDTTNEDGERVYFRVMSFIFVMACREIFWDSRVTIEH
                     SLSDGLYCEVHIDRKLKEADVENIKNKMKEIVNNDYVIEKIEVTRNEAISIFENNEMY
                     EKAELLKYKEYDSAKVYKCRNYIDHFYGYMLPSTGYIKSFDIKLYNGGVIILGPSEED
                     KTLPMKFVPQPKLSSVYYEAEEWSRLMGIDKVVSLNKIIENNEYGDIIRTFEALHEKK
                     LSQIADMIKDGNKRVVLIAAPSSSGKTSFAHRLSIHLRVNNLNPISISLDDYFIDRKH
                     TPLDEYGNYDFESIYAIDLEKFNLDLKKLLAGEEIDDIRFNFKEGKREYTGKKIKIDS
                     NQPIILEGIHGLNPILTSSIPDEDKFKIYISALTQINLDDHNRIPTADLRMIRRIVRD
                     YNFRGYSADNTILQWASVRRGEKKNIFPYQEEADAIFNSACVYELAVLKRFAKPLLEE
                     IKEDNPAHIEATRLLKFLQYFVELDDTSDIPGISILREFIGGSKIVD"
     gene            9627..10556
                     /gene="glsA"
                     /locus_tag="KPD58_001521"
     CDS             9627..10556
                     /gene="glsA"
                     /locus_tag="KPD58_001521"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBG0265298.1"
                     /translation="MNTLDETLLKEIISSNKKYTNYGQVASYIPELKNARRNDLGICI
                     IDSENNLYSAGNCSTKFTIQSISKPIVLAMALMDNDWEDVFSNVGMEPSGDPFNSIMK
                     LEINDTKKPCNPMINAGAIVTTSLINGSCLEEKEERMLSFFRKLAKNDNIGINYDVYK
                     SEKMTGDRNRAMAYLLKSDGFIRGNVEDVLDLYFKQCSIEIDSVDLARIGINLANYGV
                     DIENGEHLMSEMVSRIVKTFMMTCGMYDASGEFAIKVGIPAKSGVGGGIMASVPGRMG
                     IGVYGPALDKKGNSVAGVKVLEELSNKLKLNIF"
     gene            10654..11217
                     /locus_tag="KPD58_001522"
     CDS             10654..11217
                     /locus_tag="KPD58_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0265299.1"
                     /translation="MKEVLVLRDLECIKAIAHPRRIDILKAFDKSPLSAKQLSQLLEE
                     PHAKINYHIKMLYKVGILELVEEKIKSGIVEKYYYPSAKNVVIGNRILNFSLDNGEEK
                     EELYISKFENMSEVFYKAAEEDVLENENIVDYHDISLTHDELVELSDTMKSKIDEILN
                     KRQHNVEGSKYDIAMVIVPTLEEECPS"
     gene            11705..12541
                     /locus_tag="KPD58_001523"
     CDS             11705..12541
                     /locus_tag="KPD58_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBG0265300.1"
                     /translation="MIKLGTHLSIAKGFANAAETAVYIGANTFQFFSRNPRGGNAKEF
                     DEKDIIKFQEIRKENNFAPLLAHAPYTMNLGGTKDDVYEFATRVIDEDIRRMDSLGIE
                     YMCFHPGSHVGGGVDFGIDRIVKGLNNAIKGDENITILLETMSGKGTEIGFKFEQLKS
                     IIDKIEHKEKIGVCLDTCHIFSAGYDIVNDLDGVLEEFDKVIGIERLKTVHLNDSMMP
                     FGDKKDRHAPIGEGKIGLKALIDFMEHPSLKHLPFFLETPFDDEGHKRELKMIKEILS
                     SK"
     gene            12825..13820
                     /locus_tag="KPD58_001524"
     CDS             12825..13820
                     /locus_tag="KPD58_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBG0265301.1"
                     /translation="MRNLGNTNMKIKRVGFGGIPIQRITQDDTNLVINELEKQGINFI
                     DSARGYTISEEAIGIAIEGKRDKFFLATKSMSRDYDSMKRDVEISLNNFKTDFIDLYQ
                     FHNVKEEEYDNLFKDKMAYSALLEAKEQGKIKHIGITSHNLNTIEKAIEDGKFDTIQF
                     PYNIVEGQADEVFKKAHEKGIGIIVMKPLAGGALDNATLAIKYILSKEYIDVVIPGME
                     SVEQVRQNVAVLENLVLDEKDNKEIEEIRNSLGKKFCRRCEYCMPCAVGINIPLSFLC
                     EGYYTRYGLKEWAKEKYEVMDVKPTECIDCGLCESRCPYELPIREMLKTVVEKLG"
     gene            complement(14077..14481)
                     /locus_tag="KPD58_001525"
     CDS             complement(14077..14481)
                     /locus_tag="KPD58_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBG0265302.1"
                     /translation="MIKITIPGRPISKSNFKLHNINGQAWMPSKGKYSKYLAYENMIA
                     GFINQQYQGETVEENLITVLKLFFPNKRMGDLHNYPKSICDGIEKSGIIKNDKQLKPV
                     LLFDFIDKDNPRVEIELYPVSKYDISYNIFEK"
     gene            15578..16492
                     /gene="queG"
                     /locus_tag="KPD58_001526"
     CDS             15578..16492
                     /gene="queG"
                     /locus_tag="KPD58_001526"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBG0265303.1"
                     /translation="MDNKELKAFCNLIGLNCVGVAGVDKYDNLEKILKDRQQKGYLTG
                     MEEPIIENRINPRHIMKDANSIIVCAFPYHINRQEKNKNSNLSKYCHGKDYHIVVKDF
                     LQQICDYISKTVTDFKYKLFADNGPLVDRYLAYLSGIGYFGINNNIITDEYGSYVFIG
                     YIVNNYKFEEDKPLEKSCFKCGKCVKYCPGNAILGNYDMNPKKCLSYITQKKGDLSEK
                     EKVVLKNGKKVFGCDICQEVCPHNVEIPTTHILEFKENIIDYLDYDEIQNISNKEFKR
                     RYGDRAFSWRGRNIIKRNMEIILDKYDD"
     gene            16598..18835
                     /locus_tag="KPD58_001527"
     CDS             16598..18835
                     /locus_tag="KPD58_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0265304.1"
                     /translation="MKYNNTSEINPLDYILGQERAVEAMELGLKINNPAYNIYIAGEP
                     GTGKSTYALKVLNEYASKKNTHKDWCYIYNFENPREPIIVELEKGFGRELKKDMEKLI
                     ESLLEDFKNAFESENFEIEKNKLLDEYEIEKDMLLKQIKKYGEEKGFKLKQSKMGIVF
                     IPLKEEEDESDESDEEFYKAKRELENMAIQVVYKIRNLEEIAEKAVLELEEEIAKLVV
                     EPHIKRLCEKYENYDKIQTYLENIKKDIIEYMYLFYLDEEELKDKYDKEHFIKYKINL
                     FVDNGSSKNKESAPVVVEINPSPANLFGKAEYDYANGNIKTDFTKLLSGAFHRANGGY
                     LVLYADQLLKYTMSWEILKKTLQTKKVILETQTAIKPENMPLDVKLVLIGSHYIYDIL
                     YRYDEDFEKYFKVFVDFDSEMDKNEDNEEGIARFIAYQCDKNNLRHFTKSAVEEVIKF
                     STRLSGDLEKLSTKFNKIMEVVIEGSAYAEFRNSEYTEQCDVKKAISEKRKRINRVET
                     HMDESIENGFTLIETEGRKVGVINGLSVLSTGEYSFGRASRITATTSPGSKGIVNIER
                     EVNMSGSIHNKGVLILGGYLSENFAQDLLLSLNAYVCFEQNYGGIDGDSASSAELYVL
                     LSSLSGVSIKQNIAVTGSINQKGDIQVVGGISEKIEGFYSICKTKGLNGEQGVIIPRK
                     NQRNLVLSDEVNDAVRDGKFKIYTVERVEEAIEILTDVKFEEIKKLVKEKLISFSKIQ
                     TVSKE"
BASE COUNT         7409 a         1802 c         3512 g         6201 t
ORIGIN      
        1 gatagagtag taagaaaata aatagataaa gggatagaat aaatttaaat atggaaaatg
       61 aatatctaat aataagtgat taataaataa ttgtagatag attgtaaatt aaataaatct
      121 ttaatagaaa ggagaactat ttatatatta aatagtattt ataaatatga aaaaaggcga
      181 ttttatatgg agtggagttc ttatttgtat tattgctgtc ttagcaatac caagtagtag
      241 agaagtattt atggaagcaa caggtgcaca tccatatatt ggaggttttt taaagtttgc
      301 tgtattagct acaatgggag atttacttgg tgctagaatt ttgtataagg attggaaaat
      361 accagtagga gtattttata gagcaatagt ttggggaata ataggtgtag ttaccacata
      421 tggaatgtca atctactcat caggtgtagg tcaagcacaa gtggatggta tgcttccatt
      481 tgagggaata gcttttgcaa atgctctatt taagagtgca actatgaata tactaatgtc
      541 accaactgta tttttattcc ataagattat ggactgtttt atagataaaa aatatgaggt
      601 tggaaagggg attaaggtaa aacttaaaga tgtggtaaat attgtggact ggaatgctta
      661 ccttggtttt agtgttttaa aaactatacc atttttctgg ataccatgtc atacaattgt
      721 atttttattc cctgctgagt atagagtaat tgcttctgca tttgcatcta tagctttagg
      781 tctaatattg gctttagcta ataagtctaa agttgcagtt taggttcact gatgaagata
      841 atagtattat ttaaattagg aataactttt taaattaata ttatttgtat ttataataaa
      901 taataattta tataagaagt aatattctaa gtgatggata gtgttttaaa taataaggag
      961 taatactcta aatgagaagt atcattttaa ataataagtg agattttaaa taataagtag
     1021 gtttttcgtt agaatagcat tttattaaac ttaaaaataa atataatatt ttaaagaaat
     1081 ctaagttgct aagcactgtt tttaagattc taaaaaaaca agttataact aaaaaatggc
     1141 tgttaagact acaatactta taagtaatac ttatggtata tattagttca taacagccat
     1201 ttttatttta aattaataat tttattcaat aatttgagtt tatgttctaa atcaagcttt
     1261 aaattaaagg atttaaagaa gctattctag ttgtagctac ataaactctg gatatttcat
     1321 accatgtttt aaaatctact actccgcttt gaggtaaacc aaagattcct tggaatatct
     1381 taacagcgtt ttctgtatct gttccataga taccatcttc tataactttt ggtacagctg
     1441 gataagaatt agatatagca tttaactgat tttgtatggt tctaacatac tgcccagaag
     1501 aaccaacttg taatgtagta ccagggaatg acactggtac accacttact ataggcgctc
     1561 tttcaaatac tatctcatct ccatagaaat atcttagtat actctcatag tcatatcctt
     1621 ggtcacctaa gtctttactg ccccattgag tcatttggtc aggacattgg gatttttgtc
     1681 catcacaata ttgtgctaat agtggctgtc ttgatgtagg aggtcttttt ataaaagtat
     1741 taaaaatagc atctacaact acgttaattg gttcaaataa gtttctatta tttataaact
     1801 tatggtcgta tgctgttgtt gaagttattg taaagttata tcctttattt ctataccatt
     1861 cagtaaagac tctatttaaa gtaaatgata ttatagctat tacatttgca taaattgtct
     1921 gttcaggcca agttgaatat atttcagatg atgctatgtt tttaatgtac tctttaaatg
     1981 gaatccaata gtttggagct gatgaatctt ctggcaaacc atcatgaact actataaatt
     2041 caggcactac agggttatct aatacaacga aaccagtagg aggtggtaat ggttttaagt
     2101 tactttctgg aatcttagga ggataagtac catatagagt atgttctcct atgtcaaaaa
     2161 tcaactcact ttgtcttgag aaagaacgct tacttcttgt tctaggagac atgggaactc
     2221 cttgtcttgc ttcaattgta gctaacaact gtgttccttc tatgacaact gtctcatatc
     2281 catctgctat ggcttccact atatactggc tataaggtct aacatcagat ggttgtagag
     2341 agtaatctac atctggtgct ggtaatacta aaccagtcac ttgaccagat tcattacttc
     2401 ttaggttttg gtataaagtt gatgagcttt ggctaccatt agacattgaa tatatgttta
     2461 ctactgcatt ttgaataggt ctattgttag tagcatcaat tatgcttact gttaagaaac
     2521 catcttgcat caaatcaccc tttctttaaa tgaatttagt tcccttatat ttcaagatat
     2581 gaatatagct taaaatttgt taaataatat agatataaat ttaaattttt ttgagtattt
     2641 gagtatattt gtatttaaat aatttaaaat aatttaatgt atattttagt atgtatttaa
     2701 aacacgaata agttaaaaat aagtagatga tataaatcat aaataaatca aaaattttga
     2761 aaggaaaata atctatgaag ctagatttat ttttattagc aatagttcct atattgatag
     2821 gaatgttttg gataaggtct aaagatagat attgtagaga acccctaatc catctaatta
     2881 aatttttttt aataggtgct tttctaagtg ttataattat acttttagaa aatttactga
     2941 tgaagtttaa tgtatttgaa gggtattcag aactaatata tgtatcattt gtagttgcag
     3001 gacttgtaga agaaggtgta aaagctctga tactgattcc tgccctaatt aaagaaaaac
     3061 actttactga aaagttagat ggtattattt actctgtatt tttagcttta ggatttgcaa
     3121 caattgaaaa tatggtttat attttttctg aaagtagaaa tctagcactg caagttggta
     3181 ttaatagggc agttatctct ataccagctc atgtaatgtt tgcaattact atgggctact
     3241 atatttctaa gtataaattt gagggaaata aaaataaaag acgtgagtat ttgtttatgg
     3301 cagtgttaat tccaatttta ttacatggag tttttgattt catattgatg atagaatata
     3361 gatgggctat tatattactt atagtttatg taataatttt gtggaagata aatcttgata
     3421 aacttgagaa atatatgaat cactctaaaa aagtgttttt cggtaatctt aggaaaaaga
     3481 agaagaaata aaatttaaag gaaagacata aataattaaa gtataataaa tgacacacaa
     3541 gtaaagcaga ttgaaggggg attatagatt tgacagaatt accaaaggaa tttttagaag
     3601 atattgaaga aatactaaaa gatgaatatg atgattttat aaaaagttat gaggaaagta
     3661 agactactgg gcttagaata aatactttaa aaattgacaa gaaaaaatta tttgatttgg
     3721 agttgtttga tttgaatcaa ataccatggg cagaagaagg tttttattat gatgataaaa
     3781 ttgacagacc tggtagaaat ccactgcatg aggctggagc atactatttg caagagcctt
     3841 ctgcaatgag tgtaatacca aaggcagata ttaaagaggg tgaaaaagtt ctcgatatgt
     3901 gtgctgctcc tggtggaaaa tctacatata tactatctaa gctaaacgat acaggtttat
     3961 tggtttctaa tgaaattaat gctagtagaa ttaaggctct aggggaaaat ttagagcgat
     4021 ttggagcgag aaattgtatt ataacaaaca ccgattctaa tagcttaaga aagacgttta
     4081 atgggtattt tgataaggtt attatagatg caccttgttc tgggcaaggt atgtttagga
     4141 aggatgaggt tgccatatca gattggagtt atgcaaaggt agtagaatgt caatccatcc
     4201 aaaaagagat tataagagat ggatataaaa tgttaaagaa ggatggcatt ttaatttact
     4261 caacttgtac tttttcaagg gaggaaaatg agcatgttat aaatgagttt atcagtgaat
     4321 atccacatgc agaacttatt gaaatggaaa gattatggcc tcataagata aagggagaag
     4381 gtcattttgt tgccaaaata aaaaaattag aagaagaaga atgtaatgtc aaagaattaa
     4441 aaataaaaaa tttagataaa gaaataaagg attataggga gtttgaaaag aaatttttaa
     4501 aggttgattt ttatagtaat aaaaaaaata aattttattt aagaggagaa aatttgtacc
     4561 tacttcctga aatatatcca gatacgaaaa aactaaaagt attgagatat ggtttgcatt
     4621 taggggtttt aaagaagaat agatttgagc cttctcatgc actatctcat tacttaaaag
     4681 ttgaagatgt aaaaaatgtt gaaaacttca gtgtacagag tgaatcaatt cttaaatatt
     4741 taaaagggga tgttgtaaat tctaatgata gtagaggatg ggttttggtt tcggttgaag
     4801 gaataccttt aggttgggga aaagaatctt ctggtgtaat taagaatcat tatcctaaag
     4861 gtcttagaaa agtattttaa actattagaa aaaagaagta taatatttat attgtaggtt
     4921 tatggtagat tattttgctg tagattgact taatatttaa cacagaggag acgattttag
     4981 tgggtgaagc agttaaaaaa gaagttgtag aatggataaa agtgattgtc atagctcttg
     5041 ttttggcatt tgcaataact cgttttatag tgccaacaat agtcaaagga gaatcaatgt
     5101 atcctacatt agttgaacgt gattatttga tagttaacag aattgcgtac aaggtaggag
     5161 agccaaaata caaagatata atagtattca aaaccgactt aacagaggaa aatggaaaga
     5221 aaaaagattt agtaaaaaga gttatcgggg ttcctggtga ccatgtaaaa atacaagact
     5281 ccaaggtata tgtaaatgat aagttgttag atgagacttc ctatatacat aataatcgta
     5341 ctgatggaga tattgatatc gtagttccag aaggaaaatt atttgcaatg ggagataata
     5401 gagaaaaaag tttagatagt agatacgatg aggttggatt ggtcgacgag cataccattt
     5461 taggaaaggt tctagtcaga ttgtatccat tttctaagat aggaactatt gactaaacat
     5521 ataaaatttg gagcaatcgc tgattagctg aaatcaccag accatacaca atagttgtct
     5581 atataaaaaa gacaacacct tatttagtag acttatctaa aagatagtgt ttaagagaca
     5641 ttatcaataa agataatata actataaagg agaatttgtg aatgaatgaa actattaaag
     5701 aagagattgt agagtggata aaaataatta ttactgcact tttttttgca tttattataa
     5761 ctcgttttat aaaaccaaca ttagtaaatg gagaatcaat gtacccaaca cttaaatcac
     5821 atgattattt ggtagcaaac aggatgacat ataagttatc agaaccaaaa tgtggagata
     5881 taatgatatt taagactgat ttattacaag agaatggaag gaaaaaagag cttgtaaaaa
     5941 gggttatagg tgttcctggt gaccatctaa aaattaagga ttctaaggtt tatataaatg
     6001 gtaagttatt aaatgaagtt tcatatatac atgataatta tactgaaggc gatattgata
     6061 tggtgattcc taagggaaaa gtatttgcga tgggagacaa tagagaagtt agtttagaca
     6121 gtagatataa agaagtggga ttagtagatg aagaaaatat taaaggaaaa gttattttaa
     6181 gagtatttcc ttttacagat ataggtattt ttgagtaggt gatttgtatg gcagttggta
     6241 tagcgtcctt attgtttaat attgaggagg ctttaaacat ttgtgagagt ataaagcaaa
     6301 taactcattt agagattgga attgacaata tatctgaatg tagtgagtta tgtaaatata
     6361 aagagagaat ttctaagtta ggattatcta tagggataca tttgcctatg gaattaaata
     6421 cttgcgaaaa catagagtat atacgaaatt catggataaa ttttattgag aagatagaat
     6481 ttgaactaaa aggatttgat ttaaggtatt ttaaccttca tttgggatat gttatgacaa
     6541 atagagttag aaagaataga gacaaatatt taggaaatag tgtggatttt ttagataaat
     6601 taaatacaaa ttcatatgta tgtatagaga atacttattc aaaaggtggg gatttttcaa
     6661 atattggaaa tatatcttat gattttgaat atatatttaa gaggattaaa aattctaaga
     6721 tttgtttttg ttacgatact ggacactgtt taattgatga ggatgcttat gtaaagaact
     6781 taaaggataa aataagactg atacacttaa gtgataatga tggtatcaat gatacacatg
     6841 ttggtatagg aagaggaatt ttatctgaag aggggattaa agaggtctta acattagatg
     6901 cagaatattt agtattagaa ataaattacg aagacattga agatactata agtaaattga
     6961 ataatattgt aggagagggt taaatacctc tctttatctt gttgatatgc ctcattaatt
     7021 aaaatttata ttcatcaaaa ggaaggagat gttgaagtga aaaaaataaa cttaaaaata
     7081 gaaggaaaag ataaggaata ttcattagaa gaaaattccc ctggaatcag attgggagat
     7141 atagcaaaag aattttgtga tgagcataag ggttatataa cacttgcagt tgtagacaat
     7201 aaactaaaag agctaaattg tagagttaag aaggattgtg agataaattt tttagatact
     7261 actaatgaag atggagagag agtttacttt agagtcatgt catttatatt tgtaatggca
     7321 tgtagagaaa tcttttggga tagcagagtt acaatagaac attcactatc agatgggcta
     7381 tattgtgaag ttcatataga tagaaagctt aaagaagcag atgttgaaaa tataaaaaac
     7441 aagatgaaag agatagtaaa caacgattat gttatagaaa aaattgaagt tacaagaaat
     7501 gaagctattt ctatctttga aaataatgaa atgtatgaaa aggcagagct tttgaaatac
     7561 aaagagtatg atagtgctaa agtatataag tgtagaaact atatagacca tttttatggt
     7621 tatatgttgc catcaactgg ttatataaaa tcatttgata ttaagcttta taatggaggc
     7681 gttataattt taggacctag tgaagaagat aagactcttc ctatgaaatt tgtacctcaa
     7741 cctaagctat caagtgtata ttatgaagca gaagaatggt caagacttat gggaattgac
     7801 aaggtagtat ctcttaataa aataatagaa aacaatgaat atggagatat aataaggaca
     7861 tttgaagcat tgcatgaaaa gaagttatcc caaatagcgg atatgataaa ggatggcaat
     7921 aagagagtag ttttaatagc agccccatct tcatctggaa aaacaagttt tgctcacaga
     7981 ttatcaatac atcttagagt aaataattta aatcctattt caatttcttt agatgattat
     8041 tttatagata gaaaacatac tcctttagat gaatatggaa attatgattt tgaatcaatt
     8101 tatgcaatag atttagagaa gtttaactta gacttaaaga aacttttagc tggtgaagaa
     8161 attgatgata taagattcaa ttttaaagaa ggtaagagag aatacactgg taaaaagata
     8221 aagatagatt caaatcagcc aattatatta gaaggtatac atgggctaaa tcctatttta
     8281 acatcatcta taccagatga agataaattt aagatataca taagtgccct tactcaaata
     8341 aatttggatg accataatcg aatccctact gctgatttga gaatgataag aagaattgtt
     8401 agagactata attttagagg atatagtgct gataatacta ttttacaatg ggcatcagtt
     8461 agaagaggag aaaagaagaa tatattcccg tatcaagagg aggcagatgc tatatttaac
     8521 tctgcttgtg tatatgaatt ggcagtttta aaacgatttg cgaaaccttt gttggaagaa
     8581 ataaaggaag ataatcctgc acatattgaa gctactagat tactaaagtt tttgcagtat
     8641 tttgttgaat tggacgatac ttctgacata ccaggcattt caattcttag ggaatttatt
     8701 ggaggaagta aaatagttga ttaatgtgtg tattatagat tgttttatga agattaaatg
     8761 gcatagcaag tagtgtgatt ggtttatgtt aagtatatta agtgtaaagt ggagaattaa
     8821 attgatacag attttaaagt ttgagttgat atataaaaag gcttgtttta tacttttgta
     8881 tgaagcaagc tttttatttt aaattttact tttttgttta atattttgtt agtttatgtg
     8941 attatttttt aaaagagtaa aagagtaaaa gagtaaaaga gtaaaagagt aaaagagtaa
     9001 aagagtaaaa gagtaaaaga gtaaaaggtg ttgggttaga aatttttggt gttataaaaa
     9061 tttaaattac tggattatta gattgctgga ttgctaaatt actaggttgt taaattattg
     9121 aattattagg ttgttgaatt actaaattac taggttgtta agttactgga ttatttagat
     9181 agttagatta ctaggttgtt aaattattga attattaggt tgttgaatta ctaaattact
     9241 aggttgttaa gttactggat tatttagata gttagattac taggttgtta aattattgaa
     9301 ttattagatt gttgaattac taaattacta ggttgttaag ttactggatt attagatagt
     9361 tagattacta ggttgttaaa ttattgaatt attagattgc taaattatta ggttgttaaa
     9421 ttactggatt attagattac taaattgcta ggttgttaaa ttattgaatt actaaattat
     9481 taagctcttt aaactattta aattgtttga aaaatgaatt gattcttgta gaaaataatt
     9541 ttaaagtata atttttttta acattgtttt attatagtat aaacttgtgc taaaatagaa
     9601 tatataacat aatataagag gtgcatatga atacgttaga tgagacatta ttaaaagaaa
     9661 ttataagttc taataagaag tatactaatt atggacaagt cgctagttac ataccagaat
     9721 taaaaaacgc tagaagaaat gatttaggaa tttgtatcat agatagcgaa aataatcttt
     9781 atagtgctgg aaactgtagc acaaagttta ctatacaaag tatttcaaaa ccaattgtgc
     9841 ttgctatggc acttatggat aatgattggg aagacgtttt ttcaaacgtt ggaatggagc
     9901 caagtgggga cccatttaat tcaataatga aacttgaaat taatgacaca aaaaaaccct
     9961 gtaatccaat gataaatgca ggggctatag taaccacttc ccttataaat gggagttgtt
    10021 tagaagaaaa agaagaaaga atgcttagtt tctttagaaa gttagcaaaa aatgataata
    10081 taggaataaa ctatgatgtc tataagtctg aaaaaatgac tggagataga aacagagcta
    10141 tggcttactt attgaaaagt gatggattta tcagagggaa tgtagaagat gttctagacc
    10201 tttattttaa acaatgctca attgagatag attctgtaga tttagcgaga attggaataa
    10261 atcttgcaaa ttatggagta gatatagaaa atggtgagca tttaatgagt gaaatggttt
    10321 ctaggattgt caagacattt atgatgactt gtggaatgta tgatgcttct ggggaatttg
    10381 ctattaaagt tggcattcct gcaaaatctg gagttggggg tggtataatg gcttctgtgc
    10441 cgggacgcat ggggattggt gtttatggac ctgcgcttga caaaaaaggt aacagcgtcg
    10501 caggagttaa agttttagag gagttgtcaa ataaactcaa attaaatatt ttttaaaata
    10561 ttaagaaagg atttttttga caacaacata taatttaata tataattaaa ttgtaaagaa
    10621 atatgaatta aataaatttt ttaggagggg tcaatgaaag aggtattggt attacgagat
    10681 ttggagtgca taaaggctat tgcgcatcca cgtagaatag atattctaaa agcatttgac
    10741 aaatcgccat tgtcagcaaa acaattatct caattattgg aagaacctca tgcaaaaata
    10801 aactaccata taaaaatgtt atacaaagtt ggtattttag aattagttga ggaaaaaata
    10861 aagtcgggga ttgtggaaaa gtactattat ccaagtgcaa aaaatgtggt tatagggaat
    10921 aggatactta atttttcttt agacaatggg gaagaaaaag aagaattgta catatcaaaa
    10981 tttgaaaata tgagtgaagt tttttataag gcagcagagg aagatgtttt agaaaatgaa
    11041 aatatagttg attatcatga tatttctctt actcatgatg aattagttga acttagtgac
    11101 actatgaagt caaagataga tgaaatttta aataaaagac aacacaatgt tgaaggttct
    11161 aagtatgata tagctatggt tatagttcct actcttgagg aggaatgtcc tagttagaaa
    11221 attaattatg gattaattaa agatataata ttagagattg aacatcaaaa tttacttaat
    11281 aatcaaaaaa tagtttttgg tatgggtaaa acaatgattt ttaatctcta tttttttgtt
    11341 ttcttatacc tattgtattt gtaaataatg tgtatagtgt aagatgtata cactatctaa
    11401 gaagtgctta gataatatac aaagtatgta agattataat atttgaaatt gattaaaatg
    11461 ggtataatta aagtgtaaat attaaaatta aaaattaata taaattaaat caatgctaga
    11521 aatatgttaa aagaggaatg agattagttg ggatagaaca tcccttagag cttatgaatt
    11581 tttagtgaaa aagtactatc aaataggaat ctccatcttc atatgttggc taaatgtagt
    11641 taaggtggag tagttcaaaa ttaaaattga gtacaagtcg tagtacaaat aaaaggagat
    11701 taatatgatt aaattaggaa cacatctttc aatagcaaaa ggatttgcta atgcggctga
    11761 gacagctgta tatattggtg caaatacatt tcaatttttt agtagaaatc caagaggagg
    11821 aaatgctaaa gaatttgatg aaaaagatat aattaaattt caagaaatta gaaaagaaaa
    11881 taattttgca ccactgcttg cacatgcacc atatactatg aatctgggtg gtacaaagga
    11941 tgatgtatac gaatttgcaa caagggtgat agatgaggat ataagaagga tggactcatt
    12001 aggaattgaa tacatgtgtt ttcatcctgg aagtcatgta ggtggtggtg tagattttgg
    12061 tatagataga atagtaaaag gattgaacaa tgccataaag ggtgatgaaa atataactat
    12121 acttcttgaa actatgtcgg gaaagggaac tgaaataggt tttaaatttg agcaattaaa
    12181 aagtataata gacaaaattg agcataaaga aaaaataggt gtgtgtcttg atacatgtca
    12241 tattttttca gctggttatg atatagtgaa tgatttagat ggggttttgg aagaatttga
    12301 taaggttata ggcatagaaa gattgaaaac tgtccatcta aatgatagta tgatgccatt
    12361 tggagacaaa aaagacagac atgctcctat aggagaagga aagataggat taaaagcatt
    12421 aattgatttt atggaacatc ctagtctaaa acatttacca tttttcttag aaactccttt
    12481 tgatgatgaa ggtcacaaga gagagcttaa aatgataaaa gaaatactat ctagtaaata
    12541 aacctttatt aaaaatatgt gaaatactcc gccctcaaca taattagggt ggagatataa
    12601 tcatgaaatg tgataattta aattaatgga taggaataac aaaaattgta tacaaattaa
    12661 ctgggtataa agtatgatta tattatgaat agactgatat aaaatataat tatattatga
    12721 atagtatgta aatatattgc aaagattctc ataatttgtt gtataattaa agatgacata
    12781 tttatattat ataaaaaatt attaaaagtt aggtggggaa agatatgaga aatttaggca
    12841 atactaacat gaaaataaag agagttggct ttgggggcat tccaatacaa aggattactc
    12901 aagatgatac taacttagtt ataaatgagt tagaaaaaca aggaataaac tttattgaca
    12961 gtgcaagagg gtatacaata agtgaggaag caatcggaat tgccattgaa ggaaaaagag
    13021 ataaattttt cttagctact aaaagtatgt ccagagatta tgattcaatg aagagagatg
    13081 ttgaaataag tttaaataat tttaaaactg attttataga tttatatcaa ttccataatg
    13141 ttaaagaaga ggagtatgat aatcttttta aagataaaat ggcttatagt gcccttttag
    13201 aggcaaagga gcaaggtaag ataaaacata ttggtataac tagtcacaat ttaaatacta
    13261 tagaaaaagc tatagaagat ggaaaatttg acactataca attcccttac aatatagttg
    13321 aaggacaagc agatgaagtg tttaaaaaag ctcatgaaaa aggaattggg ataatagtaa
    13381 tgaaaccttt agctggaggt gctttagaca atgcaacact ggctattaaa tatatactat
    13441 ctaaagagta catagatgta gttataccag gtatggagag tgttgaacag gttagacaaa
    13501 atgtcgctgt acttgaaaat cttgtgctag atgaaaaaga taataaggaa attgaagaaa
    13561 taagaaattc tttaggtaaa aaattctgta gaagatgcga atattgtatg ccttgtgctg
    13621 taggaataaa tataccattg agttttttat gtgaaggtta ttatactaga tatggattaa
    13681 aagagtgggc taaagaaaaa tatgaggtta tggatgttaa acctactgag tgtattgact
    13741 gtgggctttg cgagagtaga tgtccatatg aacttcctat aagagaaatg cttaagactg
    13801 ttgttgaaaa gctaggatag attttattta ttgaaatcgg tgtattaaat ttactccgat
    13861 ttctttttat tagatgtggt cttatataca aatattaaat ttacatagtt atagctatta
    13921 tataaattta atatttgttt aatacttatt aaatatttac ttaatgcttt ttaaaaacat
    13981 ttgtttgagt atagacataa cacaattatt attgttgtat tagtatagtt tcaaatactg
    14041 gagtatcaat ataatgtcga ataacacata aaatctttat ttttcaaaaa tattatagga
    14101 tatatcgtat ttagatactg gataaagttc aatttcaacc ctagggttat ctttatctat
    14161 aaaatcaaat aaaagtactg gcttaagttg tttatcattt tttattattc cacttttttc
    14221 aataccatca caaatacttt ttggataatt atgtaaatcc cccattctct tatttgggaa
    14281 gaataatttc aagactgtta ttagattttc ttcaacagtt tccccttgat actgttggtt
    14341 tataaaacct gcaatcatgt tttcgtatgc aagatactta gaatattttc cctttgaagg
    14401 catccatgct tgaccattta tgttatgaag cttaaaatta gatttactaa taggtctacc
    14461 tggaattgtt attttaatca aatttttcac catccttata cagtatttat tataatctgt
    14521 atatgattta aaagcaaatt gttatttaaa gtgttttaca gagctttttt gtaaaatttt
    14581 ccactttaga tgtagtaata taagataagt attttgctta caaattttaa aatttgatat
    14641 atgatggaca tattcatatt agactattat aagaccttta taatattaat aagtatagtt
    14701 tagtaaagag agtaaaaaga aatccatata agtcaagtaa agacttaata aatggcaatg
    14761 taaatgggat attaattata agaaaaaatc aatctttttg cttaactata gaaaaaatgt
    14821 caaaatataa ctaatgatat ttgaagtttt agagtttata ttaaaaggta agaaggttaa
    14881 gtgggtaaaa aaggttgaat agattaaaaa ggctaagtat ctaaaagagt taagtaggta
    14941 aaaaagttaa ataagtaaat gaaaaaggct aagtatctaa aagagttagt aggtaaaaaa
    15001 gttaaataag taaatgaaaa aggctaagta tataaaggag ttagtagata aaaaaattaa
    15061 gtatctaaaa gcgttagtag ataaaaaaat taaataaata aaaaagacta agtattcaaa
    15121 agagttagta ggtaaaataa ttaaatagat aaaaagacta attatttaaa ggagttaagt
    15181 agataaaaaa gttaaataag taaatgaaaa agacgaagta tctaaaagag ttagtaaata
    15241 aaaaagatga agtatctaaa agagttagta aataaaaaag atgaagtatc taaaagagtt
    15301 agtaggtaaa aaactaaata ggtaaaaaga tgaagtatct aaaagagtta gtaggtaaaa
    15361 aattaaatag gtaaaaagac gaagtaccta aaagagttag taggtaaaaa aattaaatag
    15421 ataaaaagac gaagtatcta aaagagttaa gcaggtaaga aaattgaata gctaaaaaga
    15481 ttaagtattt aaaagagtta gtagctagaa aaggttaaaa agactaaaaa aattaaataa
    15541 atttaaaaag tcaattagat taaaaaggaa taattttatg gataataagg aattaaaagc
    15601 attttgtaat ttaataggat taaactgtgt aggagtagca ggggtagata agtatgataa
    15661 tttagaaaag atacttaagg acagacaaca aaaaggttat ttaactggta tggaagaacc
    15721 aataattgag aacagaatta acccaagaca tataatgaag gatgctaact ctataatagt
    15781 ctgtgcattt ccatatcata taaatagaca agaaaagaat aaaaattcaa atttatcaaa
    15841 atattgtcat ggaaaggatt atcacattgt agtaaaggac tttttacaac aaatttgtga
    15901 ttatatttct aaaacagtaa cagattttaa atacaagtta tttgctgata atggaccatt
    15961 agttgacagg tatttagctt atttatcagg aataggatat tttggaataa ataataatat
    16021 cataactgat gaatatggtt cttacgtatt tatagggtat atagtaaata attataaatt
    16081 tgaagaagat aaaccactag aaaaaagttg ttttaaatgt ggtaagtgtg taaaatattg
    16141 tccgggaaat gctatattgg gaaattatga tatgaacccc aaaaaatgtt tatcatatat
    16201 aactcaaaaa aaaggagatt tgtcagagaa agaaaaagtt gttttaaaga atggcaaaaa
    16261 ggtatttgga tgcgatattt gtcaagaagt atgtccacat aatgttgaaa taccaacaac
    16321 tcatattttg gaatttaaag aaaatataat agactatttg gattatgatg agatacaaaa
    16381 tatttcaaat aaagaattta aaaggagata tggagataga gcttttagct ggagagggag
    16441 aaatataata aagagaaata tggaaataat tttagataag tatgatgact aataataaga
    16501 gtctgtttca tatactttaa tgtgtattta ctttaacact tgttgtgtaa aagtcatatt
    16561 atatcattaa agttaaatat ggggaggtgg gggaatattg aaatataata atacatctga
    16621 aataaatcca ctagattata tattgggtca agaaagagct gtagaagcta tggaattagg
    16681 gttgaaaata aataatccag cgtacaacat atatatagct ggtgaacctg gaactggtaa
    16741 aagtacttat gccttaaagg ttttaaatga gtatgcatcc aaaaaaaata cgcataaaga
    16801 ttggtgttat atatataatt ttgaaaaccc tagagagcct attatagttg aattagaaaa
    16861 ggggtttgga agagaactta aaaaagatat ggagaaatta attgaaagtc ttttagaaga
    16921 ttttaaaaat gcgtttgaaa gtgaaaattt tgaaatagaa aaaaataaac ttctggatga
    16981 atatgaaata gaaaaagata tgcttttgaa gcaaattaag aaatatggag aagaaaaagg
    17041 atttaaatta aaacagagta aaatgggaat agtttttatc cctttgaaag aagaggaaga
    17101 tgaaagtgat gagtctgatg aagagtttta caaagctaaa agagaactag aaaatatggc
    17161 gatacaggtc gtatataaaa ttagaaactt agaagaaata gctgaaaaag cagtgttaga
    17221 gcttgaagaa gagatagcta aacttgtagt agaacctcat atcaagagat tgtgtgaaaa
    17281 atatgaaaat tatgataaga ttcaaactta tctggaaaat ataaagaaag atataataga
    17341 atatatgtat ttattctatt tagatgaaga ggagttaaaa gataagtatg ataaagaaca
    17401 ttttattaaa tataaaatta acctatttgt agacaatgga agctcaaaga ataaagaatc
    17461 agcacctgtt gtagtagaga taaacccaag tccagcaaat ctatttggaa aggcagagta
    17521 tgattatgca aatggaaata taaagactga tttcacaaaa ctattatcag gagcatttca
    17581 tagggctaat ggtggttatt tagtacttta tgcagaccaa ttactaaaat atacaatgtc
    17641 ttgggaaatt cttaaaaaga ctttgcagac taaaaaagta atattggaaa cacaaacagc
    17701 aattaaacca gaaaatatgc cattggatgt taaattagta ctgattggaa gccattatat
    17761 atatgatatt ttatatagat atgatgaaga ttttgaaaaa tattttaaag tatttgtaga
    17821 ctttgatagt gaaatggata aaaatgaaga taatgaagaa ggaatagcaa gatttatagc
    17881 ataccaatgt gataagaata atttaaggca ttttactaaa tcagcagttg aagaggttat
    17941 taaatttagc actagattat ctggtgattt agaaaaacta tcaactaagt ttaataaaat
    18001 aatggaagtt gtaatagaag gaagtgctta tgcagagttt agaaattcgg agtatacaga
    18061 gcagtgtgat gttaaaaaag caataagtga aaagagaaaa agaatcaata gagtggaaac
    18121 acatatggat gaatcaattg aaaatggatt tactctaata gaaacagagg gaagaaaagt
    18181 aggcgtgata aatggtctgt cagtcttaag tacaggagaa tactcttttg gtagagcttc
    18241 aagaattaca gcgaccacta gcccaggaag taaaggcata gtaaatatag aaagagaagt
    18301 aaatatgagt ggctctatac ataacaaagg agtacttata ttaggtggct atctttctga
    18361 gaattttgca caggatttac tattgtcact aaatgcatat gtttgttttg aacaaaatta
    18421 tggtggaata gatggagata gtgcatcttc tgcagaatta tatgtactat tatcatctct
    18481 tagtggagta tcaattaaac aaaatattgc tgttacaggt tctataaatc aaaagggtga
    18541 tatacaagta gttggtggta taagtgaaaa gatagaagga ttttattcta tatgtaaaac
    18601 taaagggtta aatggtgagc aaggtgtcat aattcctaga aaaaatcaaa gaaatctcgt
    18661 tttatcagat gaagttaatg atgcagtgcg agatggaaaa tttaagatat atactgttga
    18721 aagagtagaa gaggctatag agatattgac agatgttaaa tttgaagaaa taaagaaatt
    18781 ggtaaaagaa aaattaatat ctttcagcaa gatacaaact gtgtcaaaag aataagttta
    18841 ttatagaatt taatgggtat ttatgtataa taaaaagcaa gaactttaat aggtttttgc
    18901 tttttttgta aatatatata aatt
//
LOCUS       DAELLV010000053        18541 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.053, whole genome shotgun sequence.
ACCESSION   DAELLV010000053 DAELLV010000000
VERSION     DAELLV010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18541
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.053"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..321
                     /locus_tag="KPD58_001528"
     CDS             <1..321
                     /locus_tag="KPD58_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBG0265305.1"
                     /translation="VEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDS
                     EITQYSFCTNGSHYAGEAGIRTIGFGPSKENLAHTIDEYIELDQLFKGTEGYYGILKS
                     VYKK"
     gene            337..1920
                     /locus_tag="KPD58_001529"
     CDS             337..1920
                     /locus_tag="KPD58_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HBG0265306.1"
                     /translation="MRTLIKHGLIVDGNKTPAYEGDILIENEKILKISQDLNEEADKV
                     IDAKGRVICPGFIDTHSHSDLVILVNPYNEVKIRQGITTEVLGQDGISMAPLPQEHIS
                     SWRKNLAGLDGESDEIDWKYETTENYLKMMDYNGVGLNETYLVPHGNVRMEAMGLEDR
                     PATKEEIQKMCEITERELKAGAIGLSTGLIYIPCAYSLTEEIIEMCKVVAKYDGVFVV
                     HQRSEADTILTSMEEIIEIGKQSGVKVHFSHFKVCGKANWKYIPQVIELLEKAEKEGI
                     RVSFDQYPYAAGSTMLGVVLPPWAHSGGTDKLIERLSDENERAKMKKDIANGIEGWDN
                     FIEFAGIDQIFVTSVKTEKNKDTIGKSLLEIGKMRGKDPLDATFDLLKEEENAVGMVD
                     FYGLEEHIIGFMKRDEQNVCTDGLLAGKPHPRAYGSFPKILGRYVRELNVLTIEEAVY
                     KMTKKAARSFSIKDRGELKEGYFADIVIFDKDTVSGCDDFINSMQYPTGIDYVIINGN
                     CVVEEGKYNKIKAGKVLKN"
     gene            1948..3276
                     /gene="ssnA"
                     /locus_tag="KPD58_001530"
     CDS             1948..3276
                     /gene="ssnA"
                     /locus_tag="KPD58_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HBG0265307.1"
                     /translation="MILVGNGRLISQDSLNPYMEDGCVVINDNLIEDIGTTEEMKVKY
                     PVHEFIDAGKKIIMPGLINSHMHIYSSFARGMAVPGKPSENFLEILNNLWWRLDKQLT
                     LEDTKYSAYATYIECIKNGVTTVYDHHASPNAIEGSLFTISDVAKDLGIRTCLCYEVS
                     DRDGGNTIQQGINENVNFIKYAQKDDTDMIKGMFGLHAAFTLSDDTLNKCASEMAGLD
                     TGYHVHVAEGLADLQYNVDKYGKRVLERFNDFGVLKEKTIAVHCIYISAEEISLLNKT
                     NTFVVHNPESNMGNAVGCSPAIELLKRGVTVGLGTDGYTSDMFESLKVANIIHKHHLK
                     DPRVGFEESQTMLFDNNRKIAKAYYKNDLGVLKKGAYADVIVVDYIPHTPLTENTIGG
                     HTIFGMSGRSVDTTIINGKVLMKDRKLINIDEESILAKSRELSQNLWNRI"
     gene            3421..4773
                     /locus_tag="KPD58_001531"
     CDS             3421..4773
                     /locus_tag="KPD58_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBG0265308.1"
                     /translation="MSRDKSPKIAPVDERLPVSKSWVFSLQHVLAMCAGAVAVPMMIG
                     SAARLDHASIVFLINACLFMAGIGTLIQSVGIGNIVGAKIPVIEGTSFAAVAGMTAIA
                     SSYSDSQVAIQTVFGSVIVAGLFCILLAPVFEKLIRFFPSVVTGTVVMVIGISLFPVG
                     IKWITDNKLEAVEPNSLALALAVLAITLLSFKFLKGIWNSTAILVGIVLGTLLSMAVG
                     MADFSAVNGVGWLNLNVPLKFGMPRFEPSAILSLCLIMLVLMTESVGNMIAIHEMVDK
                     EVTGKNIKRGLFGDGISTALSGIFNSFPITPFGQNTGIVGLTGIKSRFIAVYAGVILL
                     VLGFLPKFAAIIGAIPKPVLGGVGFAMFGMVMVGGVKTLSKVKFDGTKNGVIVAVSVG
                     LSMIPLTNPDFYHNFPTWVQTIFHSGITTGSLSAVLLNIFFNELGSKKEKNNKVEEAK
                     "
     gene            4965..6074
                     /locus_tag="KPD58_001532"
     CDS             4965..6074
                     /locus_tag="KPD58_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG0265309.1"
                     /translation="MANLKVNLYGMEFLNPIMTAAGPGAKDGALCQEAAKGGAGAICT
                     KTISVLPADVPRPCMAKTNSGFLNTELWSELPKEQWIEEEYKKVKETGLPVIVSMGYS
                     AEQIAEVAPLVKPYADAVELSTHYVGTDVAPIVNALKAAKAVLDVPVFMKMSPHTDIQ
                     TIAKAVEEAGADGLVMINSFGPCMAIDVNTGYPIMGSKAGYGWLSGAPIRPLAIRCIY
                     EASQVVNIPIIGVGGITCGKDVAEMLMAGASAVQVCTEAILKGPSIYGKIAKELNEFL
                     DENGYADVNEIKGLTHKKMAARGFRTHSVAPVVDDNKCIKCKQCVTSCVYEALEVTDK
                     LNIDADKCFGCGLCVTRCPKGALSICYNQVEAMNE"
     gene            6357..7748
                     /gene="hydA"
                     /locus_tag="KPD58_001533"
     CDS             6357..7748
                     /gene="hydA"
                     /locus_tag="KPD58_001533"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HBG0265310.1"
                     /translation="MGIVLKGGKVVTSEKTYCADVRIEDEKIVDIGLDISKENDEIVD
                     VSDSYVIPGGIDTHTHFDLDTGVTKTADNFETGTKAAIVGGTTTILDFATQAKGCSLK
                     DGLEEWHEKAKDKSYSDYGFHMAITDWNDSVCNEMEDMVKEGVSSFKLYMAYKGSLQV
                     DDGVIFEALRKAEEIGGIIGFHCENGDIICELVDKAKSENNLSPKYHQLTRPAIMEAE
                     ATSRLMKIAEVANSKVYVVHLSCKESLEEVLKAKQRGVDVIVETCPQYLLLNDELYDL
                     DGFESAKYVMSPPLRNKENNEILWNAILDNQIDTIGTDHCSFNYKGQKELGINDFSKI
                     PNGSPGVEHRMSLLYTYGVCKNKISINKMVEVTSTNPAKLFGMYPNKGTIEIGSDADI
                     LVLNPNKKSKIEAKKQLQNVDYTPYEGYEVDCQIEHVYLRGKEVVRDGELICNTPSGR
                     YINRRLTKGAREW"
     gene            7742..10303
                     /gene="xdh"
                     /locus_tag="KPD58_001534"
     CDS             7742..10303
                     /gene="xdh"
                     /locus_tag="KPD58_001534"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HBG0265311.1"
                     /translation="MVEFILNGEKVVAKEGKKLLDFLREDMNITSVKNGCSEGACGTC
                     MVIIDGKATKACVFKTDKLAGKNITTIEGLSERERDVFAYSFASQGAVQCGFCIPGMV
                     ISSKALIDVNNNPTEDDIKKALKGNICRCTGYVKIIKAVDLAAKIFRENIDVPKVNCK
                     GLVGENLPRVDAVGKALGYSEYVDDMRIEGMIYGSAVRSKYPRALVKKIDIEEAKAYP
                     GVVAVLTAKDIPGTIKVGHLKKDWDTLIPEGEITRYLGDGIVLVAAETREALEEAKKL
                     VKIDYEELTPLSTTADALREDAPKIHETGNILVKEHLVRGNAKEKIENSKYVVTKRYT
                     TPATEHAFLEPECAVAGPYEDDGVIIYSTDQGVFATQHECADMLGLPYEKVRVINKMV
                     GGGFGGKEDMSVQHHAALLAYHTKRFVKVQLTRKESIIVHPKRHATEMEFTTACDENG
                     YLTGMKATIISDTGAYASLGGPVLQRLCTHAAGPYNYQDIDITGTAVYTNNPPGGAFR
                     GFGVTQSCFATECNLNLLAEMVGISPWEIRYKNAIRPGQVLPNGQFADEGTAMAETLE
                     AVKEEYEKHPRAGIACAMKNSGVGVGIPDVGRCRLVIKDGKVHIRTSASCIGQGLGTV
                     MTQVVCETANLLPENIVHEVPDTHLTPDSGNTTASRQTVFTGEATKVASNKLKEALDS
                     GKTLLDLEGEEFYGEYESITDKMGCDKENPVSHVAYGYATQVVILDENGKLEKVVAAH
                     DVGRAINPISLEGQIEGGVVMGLGYALTEDYPVVNSVPTAKFGTLGLFKSTDIPEIQA
                     LIVEKNKDLLAYGAKGVGEICTVPTAPAVQNAYYVFDKEFRTELPLQNTPYSKKK"
     gene            10455..11801
                     /gene="rpoN"
                     /locus_tag="KPD58_001535"
     CDS             10455..11801
                     /gene="rpoN"
                     /locus_tag="KPD58_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBG0265312.1"
                     /translation="MNFNNSLELTQSQKLIMTTQLKQSLSILNMSKLELEEEIKREAE
                     NNPLVEVEKSGEINWEEYIKDMDRSRRLDRTEISYNPDNEVNLENLVKYSSNLYEDLK
                     FQISLYKLTDKELEVCEYIIDSLDEDGYLRTEEKVIVDTLKIDEELFEKCLISVQQLE
                     PSGVGARSLSECLMIQMASLGIYNEILEEIVTKDLNLIANNKYKEISKKYNMSLQKCV
                     ELINIIKTLDPKPGRTCSVEKSVYIQPDVTVEKIDDEFIVYLNEKDSYQIRINSYYKD
                     ILKNSQSDESAKEFIKERLNSATGLMKNIESRKTTVLKIAEEIVKAQDEFLRKGTKYI
                     KPLKMKDIAEKLEFHESTISRGVNGKYMLTPFGVYEFRYFFSSAIETENNEMASSTSI
                     KKIIKETIKDENKKKPLSDDHISKLLKEKGINVARRTVAKYREELGIPSSSKRKEY"
     gene            12599..13621
                     /locus_tag="KPD58_001536"
     CDS             12599..13621
                     /locus_tag="KPD58_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG0265313.1"
                     /translation="MKNLLEIQKKLIPQAIELMERRYSILRQISLSEPIGRRTLSNVL
                     EISERIIRSETEFLKEQGLIDVAVSGMTITKEGSDLLDKLKDIMSDIMGLSKLQDRVR
                     EKLGIKRVILVPGSCDENGSLLKDVTRYGCEYFLGILKDGDIVSITGGSTMLEFSNVI
                     KTDKRYPNSTIVPARGSMGTDVEMQSNNIVASISKKLHSNCKLLHIPDELDESAMKTL
                     SQVPEIKTTLEYIKRTDILVFSIGRADVMAKRRKLPEDKVDEILSKNAVGEAFGYYFN
                     KDGEIVYKLNTVGIDLETVKQVKETIAIFAGTEKVEALLAISNMHKNMVLVTDEESAY
                     KILSLL"
     gene            13681..14688
                     /gene="gap"
                     /locus_tag="KPD58_001537"
     CDS             13681..14688
                     /gene="gap"
                     /locus_tag="KPD58_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBG0265314.1"
                     /translation="MVKVAINGFGRIGRLALRKMMEQQDKFEVVAINDLTDAKMLAHL
                     FKYDTAQGRFNGEIEVKEGAFVVNGKEIKVTAERNPADLPWAELGVDIVLECTGFFTS
                     KDKAEAHIQAGAKKVVISAPATGDLKTIVFNTNSDILDGSETVISGASCTTNCLAPMA
                     KVLNDKYGIEKGLMTTIHAYTNDQNTLDGPHPKGDLRRARAAAGNIVPNTTGAAKAIG
                     LVIPSLKGKLDGAAQRVPVVTGSITELVCTLGKNVTVEEINAAMKEASNESFGYTEEM
                     LVSSDIIGISYGSLFDATQTKVMEVDGKQLVKVVSWYDNEMSYTSQLIRTLGYFAQLA
                     K"
     gene            14910..16112
                     /locus_tag="KPD58_001538"
     CDS             14910..16112
                     /locus_tag="KPD58_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBG0265315.1"
                     /translation="MSMLNKKTIEDIDVCGKKVLVRCDFNVPLQDGVITDENRLNGAL
                     PTIQYLISKGAKVILCSHLGKPKGEAKPELSLAPVAKRLSEMLGKEVVFAADDNVVGE
                     NAKKATEKMENGDVVLLENTRYRKEETKNEENFSKELASLAEIFVNDAFGTAHRAHCS
                     TVGAGEFLQERVCGYLIQKELKFLGEAVANPVRPFTAILGGAKVSDKLAVINELLEKV
                     DNLIIGGGMAYTFLKAQGYEVGTSLLEIDKVEYAKEMMEKAKNKGVNLLLPVDVVMAD
                     HFAPDATPIVTEDANVKEDYMGLDMGPKTIANFVKTIKESKTVVWNGPMGVFEFENFA
                     NGTLSVARAMAELTDATTVIGGGDSAAAVNQLGFGDKMTHVSTGGGASLEFLEGKELP
                     GIAALDNK"
     gene            16138..16881
                     /locus_tag="KPD58_001539"
     CDS             16138..16881
                     /locus_tag="KPD58_001539"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBG0265316.1"
                     /translation="MRKPIIAGNWKMHKTIKEALEFVNEVKDKVNSDKVEAVICAPFT
                     LLKDLKEATKGTNIKIGAQNMHFEEKGAFTGEVSPLMLKEIDMDYVVIGHSERRQYFN
                     ETDETVNKKVLKALEVGIDPILCVGETLEQREAGKTKDVCKVQVEKALENVLKDDLAK
                     VVVAYEPIWAIGTGKTATAEDANDVISYIREVIKGLYGELANEVRIQYGGSVKPSNVA
                     EIMGQSDIDGALVGGASLASNDYLDLVNF"
     gene            16890..18422
                     /locus_tag="KPD58_001540"
     CDS             16890..18422
                     /locus_tag="KPD58_001540"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003228330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HBG0265317.1"
                     /translation="MMKKPVALIIMDGFGYNKDVKGNAIAESKTPNLDRIKKEYPNTL
                     INASGLDVGLPDGQMGNSEVGHTNIGAGRIVYQDLTRITKSIKDGDFFTNKVLCEAMD
                     NAKENSLHVMGLLSDGGVHSHIDHLKAIIKMAKDKGVQKVYVHAFTDGRDTDPQSALE
                     YAKEVQASMDEIGVGEFATVSGRYYAMDRDKRWERVELAYNAMVRGIGEKANSIEEAI
                     QNSYDDGKNDEFIMPTVIMKDDKPAGSIKENDSIIFFNFRPDRARQITRALVCEEFDG
                     FKREDIKNFFVCLTEYDITIENVHIAFGPQSLANTLGEYLAKNGKTQLRAAETEKYAH
                     VTFFFNGGVEEPNKGEERLLIPSPKVATYDLKPEMSAYELTDKALDKLGEDKFDFIVL
                     NFANPDMVGHTGSIEAAIKAVETVDTCVGKLIDKIVELGGSAIITADHGNAEYMLDPE
                     TGKTVTAHSINPVPFIVVGQEYESAKLLDGGRLSDIAPTVLDMMKLEKPEEMTGHSLI
                     SK"
BASE COUNT         7428 a         2146 c         3731 g         5236 t
ORIGIN      
        1 gttgaaaaag ctgattgcta tacaggaaat actatagaaa gtgaaagatt cttcccaggt
       61 tggttatatg atgaagaaga tgaatttgtt caagcagcgt ataagggatt aaaagaagct
      121 ggaatagatt cagaaataac tcaatactca ttctgtacga atggaagtca ctacgcaggt
      181 gaggctggaa taagaactat aggatttggt ccttctaagg aaaatcttgc tcatacaata
      241 gatgagtata tagaattaga ccaattattc aaaggtacag aaggatatta tggtatatta
      301 aaatcagttt ataaaaaata attaggagat ggttttgtga gaactttaat aaaacatgga
      361 cttatagtag atggaaataa aacaccagca tatgaaggag atattttaat agaaaacgag
      421 aaaatattaa aaatatcaca agatttaaac gaagaagctg acaaagttat agatgcaaaa
      481 ggtagagtta tatgtccagg tttcatagat actcatagcc attcagacct agttatttta
      541 gttaatccat ataatgaggt aaagattaga cagggaatta caacagaagt gttgggccaa
      601 gatggtatat ctatggcccc tctacctcaa gagcatatat cttcttggag aaaaaattta
      661 gcaggtttag atggtgaaag tgatgaaatt gattggaaat atgaaacaac agagaattat
      721 ttaaaaatga tggattacaa tggggtaggt ttaaatgaaa cttatttagt acctcatgga
      781 aacgttagaa tggaagctat gggacttgaa gatagacctg ctactaaaga agaaattcaa
      841 aaaatgtgtg aaatcacaga gagagaatta aaagcaggag ctataggatt gtctactgga
      901 cttatatata taccttgtgc atactcattg actgaagaaa ttatagagat gtgtaaggta
      961 gtagcaaaat atgacggagt atttgtagtt caccaaagaa gtgaagcaga tacaatttta
     1021 acttcaatgg aagaaatcat agaaattgga aaacaatcag gagtaaaagt tcatttttca
     1081 cattttaagg tttgtggtaa agcaaactgg aagtatatac cacaagttat agagctttta
     1141 gaaaaagcag aaaaggaagg aataagagta tcttttgacc aatatccata tgcagcagga
     1201 agtacaatgc ttggagtagt tttacctcct tgggcacatt ctggaggaac tgataagtta
     1261 atagaaagat taagtgatga aaacgaaaga gcaaaaatga aaaaagatat agcaaatgga
     1321 atagaaggct gggataattt tatagaattt gctggaatag accaaatatt tgtaacaagc
     1381 gtaaaaacag aaaagaataa agatacaatt ggtaagagct tattagaaat tggaaaaatg
     1441 agaggaaaag acccattaga tgcaacgttt gatttattaa aagaagaaga aaatgcagtt
     1501 gggatggtag atttctatgg attagaagaa catataatag gattcatgaa aagagacgag
     1561 caaaatgtgt gtacagatgg tcttttagca ggtaaaccac atccaagagc ttatggttct
     1621 ttccctaaaa tactaggaag atatgttaga gagttgaatg tgttaacaat cgaagaagct
     1681 gtttataaaa tgactaaaaa ggcagctaga agctttagta taaaagatag aggcgaatta
     1741 aaagaaggat attttgcaga tatagttata tttgacaagg atacagttag tggttgtgat
     1801 gattttatca actctatgca gtaccctaca ggtatagatt acgtaattat aaatggaaat
     1861 tgtgttgttg aggaaggaaa atacaataaa attaaagcag gaaaagtact taaaaattaa
     1921 atcttagctt taaggaggaa taaatttatg attttagtgg ggaatgggag gctaataagc
     1981 caagattctt taaatcccta tatggaagat ggatgtgtag taataaatga taatctaatt
     2041 gaagatatag gtactacaga agaaatgaaa gttaaatatc cagtacatga atttatagat
     2101 gcaggcaaga aaataatcat gccaggtcta ataaattcac atatgcatat atacagctca
     2161 tttgctagag gaatggcagt tccaggaaaa ccttctgaga actttttaga gatactaaac
     2221 aatttatggt ggagacttga caaacagctt acactggaag atacaaaata cagtgcatat
     2281 gcaacgtata tagaatgtat aaaaaatggt gtaacaacag tttatgacca tcatgcaagt
     2341 ccaaatgcaa ttgaaggcag tttgtttaca atatcagatg ttgcaaagga tttgggaatt
     2401 agaacgtgtc tttgttatga agtgtctgat agagatggtg gaaatacaat ccaacaaggt
     2461 atcaatgaaa acgtaaactt tataaaatac gctcaaaaag atgatacaga catgataaaa
     2521 ggaatgtttg ggcttcatgc tgcatttaca ctatctgatg atacattaaa taaatgtgct
     2581 tcagagatgg ctggtcttga tacaggttac catgtacatg ttgctgaagg actagcagac
     2641 ttacaatata atgtagataa atatggaaaa agggttttag aaagattcaa tgattttgga
     2701 gtactaaaag aaaaaacaat agcagttcat tgtatatata taagtgcaga agaaatcagt
     2761 cttttaaata aaacaaatac atttgtagtt cataatccag aatctaatat gggaaatgca
     2821 gttggatgtt ctcctgcaat agagttatta aaaaggggag ttacagtagg attaggaact
     2881 gatggatata ctagcgatat gtttgaatca ttaaaagttg ccaatataat tcacaaacat
     2941 cacttaaaag acccaagagt tggatttgaa gaatcacaaa caatgttatt tgataacaat
     3001 agaaaaatag ctaaagctta ttataaaaat gacctaggag tacttaaaaa aggtgcttat
     3061 gcagatgtta tagtagttga ttatatacca catactccat tgacagaaaa tacaataggg
     3121 ggacacacta tttttggaat gagtggaaga agcgtagata caactattat aaatggaaaa
     3181 gtacttatga aagatagaaa gttaataaac atagatgaag aaagtattct agcaaaaagt
     3241 agagaattaa gtcaaaatct atggaataga atttaaataa ttgatattaa tatacaagta
     3301 agaaacaaga atttagatgt taaaaacaga gcttaaacaa aaataggaat ttaaatgaag
     3361 tatataaata aaaaatatac ggaatagaat attaaattaa atatagaagg gagacccaaa
     3421 atgtctcgag ataaaagccc taaaatagct ccagtagatg aaaggttacc tgtatccaaa
     3481 tcttgggtat tttcactaca gcacgtttta gcaatgtgtg caggagcagt agcagttcct
     3541 atgatgatag gaagtgctgc acgactagat catgctagta ttgttttttt aataaatgct
     3601 tgcttgttta tggcaggtat aggaacactc atacaaagtg ttggtatagg aaatattgta
     3661 ggtgctaaaa taccagtaat agaaggaact agttttgccg ctgtggcagg tatgacagca
     3721 atagcctcat catatagtga ttcacaagtt gcaatacaga cagtatttgg ctctgtaata
     3781 gtagctggat tgttttgtat attacttgct ccagtatttg aaaaattaat aagatttttc
     3841 cctagtgtag ttactggaac agttgttatg gtaataggta tttcactatt tccagttggg
     3901 ataaaatgga taactgacaa caaattagaa gcggttgaac caaactcatt agcattagct
     3961 ttagcagtat tggcaataac tttactttca tttaaattct taaaaggaat ttggaatagc
     4021 acagctatat tggtaggtat agtacttggt acactacttt ccatggcagt tggaatggca
     4081 gattttagtg cagtaaatgg agttggttgg cttaacttaa atgtaccttt aaaatttgga
     4141 atgcctagat ttgaaccatc tgcaatacta tcattgtgtt tgattatgtt ggtacttatg
     4201 acggaatcag ttggtaatat gatagctatt catgaaatgg tagacaaaga agtaacaggt
     4261 aaaaatatta aaagaggtct atttggagat ggaatatcta cagcattatc tggaatattc
     4321 aattcatttc caataactcc atttggtcaa aatacaggaa tagtaggtct tacaggtata
     4381 aaatcaagat ttatagcagt ttacgcaggg gttatattgc tagtacttgg attcttacca
     4441 aaatttgcag caataatagg agcaatacct aagcctgttc ttggaggcgt aggattcgca
     4501 atgtttggaa tggtaatggt tggaggagta aaaactttaa gcaaagtaaa atttgatgga
     4561 actaaaaatg gggttatagt agcagttagt gttggtttgt ctatgatacc actaacaaat
     4621 cctgatttct atcataattt cccaacttgg gtacaaacaa tattccatag tggaataaca
     4681 acaggtagtt tatcagcagt gcttttaaac atattcttca atgaactagg ttctaaaaaa
     4741 gaaaaaaata ataaagttga agaagcaaaa taaaaataaa acaagtaaaa atctatattg
     4801 acttaaagtc attaagtatt aataaaaata attcagagat attaataatt caacaaaact
     4861 atcttatatt ttaaataata taaaaaagta aataaataac gaatacaaat aaaagaatac
     4921 aatctaaata ttaaaaatat ttaatttaag ggagagataa taatatggct aatttaaaag
     4981 ttaatttata tggaatggaa tttttaaacc caattatgac agcggcagga cctggagcaa
     5041 aagatggtgc tctctgtcaa gaggcagcaa aaggtggagc aggagctata tgtacaaaaa
     5101 ctatatctgt attaccagct gatgtaccaa gaccatgtat ggcaaaaact aatagtggtt
     5161 ttttaaacac agaattatgg tcagagcttc caaaagagca atggatagag gaagaataca
     5221 aaaaggtaaa agaaacaggg cttccagtaa tagtaagtat gggatattca gcagagcaaa
     5281 tagctgaggt tgctccttta gtaaagcctt atgcagatgc agttgaatta tctactcatt
     5341 atgtaggtac agacgttgca ccaatagtaa atgctttaaa agctgcaaaa gctgttttag
     5401 atgtaccagt atttatgaaa atgagtcctc atactgacat tcaaactatt gcaaaagctg
     5461 ttgaagaagc aggtgcagat ggacttgtaa tgataaactc atttggacca tgtatggcaa
     5521 tagatgtaaa tacaggatac cctataatgg gaagtaaagc tggatacgga tggttatcag
     5581 gtgcaccaat aagacctcta gcaataagat gtatatatga agcttctcaa gttgtaaata
     5641 taccaataat cggtgttggt ggaatcactt gtggaaaaga cgtggctgaa atgttgatgg
     5701 ctggagcaag tgctgttcaa gtatgtacag aagcaatcct taaagggcct tctatctatg
     5761 gaaaaattgc taaagaatta aatgaatttt tagatgagaa tggatatgct gatgttaatg
     5821 aaataaaagg attaactcat aagaaaatgg cagctagagg gtttagaact cattcagtag
     5881 ctccagtagt ggatgataac aagtgtatca aatgtaagca atgtgtaaca agctgtgtat
     5941 acgaagcttt agaagttaca gataagttga acatagatgc agacaagtgt tttggatgtg
     6001 gattatgtgt aactagatgt ccaaaaggag ctttaagcat ctgctacaat caagtagaag
     6061 ctatgaatga gtaataatta tatagaattt tggtaattaa cttagagtaa taggtattag
     6121 gataataaaa tatatcagat aaacaaacac atattatgca ttaataatac agtctaaagt
     6181 taataatttt attatattga tttaactaaa tattagttta gctgaatatt aatttaatta
     6241 ggtattggtt taatcttaat atagatttac tacgtataag tgaactaact agattacttt
     6301 aagttaattt tttcatataa aataaattga tacattacaa gtaaggtgga gtctatatgg
     6361 gaattgtttt aaaaggtggg aaagtagtaa caagtgaaaa aacttactgt gcagatgtaa
     6421 gaatagaaga tgaaaaaata gtcgatatag gtcttgatat atcaaaagaa aatgatgaaa
     6481 tagttgatgt tagtgattct tatgttatac caggaggtat agatacacac acacactttg
     6541 acttagatac aggcgttact aagacagcag ataactttga gacaggaaca aaagctgcta
     6601 tagtaggagg aactactact atattggatt ttgctacaca agctaaagga tgttctttga
     6661 aagacggatt agaagaatgg catgaaaaag ctaaggataa atcctattca gattatggct
     6721 ttcatatggc tataacagat tggaatgact cggtttgtaa tgaaatggag gatatggtga
     6781 aagagggagt atcttcattt aaactgtata tggcgtataa aggaagcctt caagttgatg
     6841 atggtgtaat ctttgaagca ttgagaaaag cagaagagat aggaggaatt attggtttcc
     6901 attgtgaaaa tggggacata atatgtgaac ttgtggataa agcaaagtca gaaaataatt
     6961 tatctccaaa atatcatcaa ttaactagac cagctataat ggaggcagaa gctacatcaa
     7021 gacttatgaa gatagctgaa gttgcaaatt ctaaagtata tgtagttcac ttaagctgta
     7081 aagaatcctt agaggaggtt ttaaaagcta agcaaagagg tgtggatgta atagttgaaa
     7141 cttgtcctca ataccttcta ttaaatgatg aattatatga tttagatgga tttgaaagtg
     7201 ctaagtatgt aatgtcacca cctttaagaa ataaagaaaa taatgaaata ttatggaatg
     7261 ctattttaga taatcaaata gacactatag gaacagacca ttgttctttt aattataagg
     7321 gacagaagga acttgggata aacgatttca gcaagatacc aaatggttct ccaggtgtag
     7381 aacataggat gagtctttta tacacatatg gagtctgtaa aaataaaata tctatcaaca
     7441 agatggtaga agtaacatca actaatccag caaagttgtt tggaatgtat cctaataaag
     7501 gaactataga aataggcagt gatgcagata tattagtctt aaatccaaac aaaaaatcca
     7561 aaatagaagc aaaaaaacaa ttacaaaatg tagattatac accttatgaa gggtatgaag
     7621 tggattgtca aatagaacat gtttatttaa gaggaaaaga agtagttaga gatggagaat
     7681 taatttgtaa tactccaagt gggagatata taaataggcg tctaacaaag ggggctagag
     7741 aatggtagaa tttatattaa atggagagaa agttgttgca aaagaaggca aaaaactttt
     7801 agacttcctt agagaagata tgaatataac ttcagttaaa aatggatgtt cagaaggtgc
     7861 ttgtggaaca tgtatggtta taatagatgg taaagctact aaagcatgtg tatttaaaac
     7921 tgataaatta gctgggaaaa acataacaac tatagaggga ttatcagaga gagaaagaga
     7981 tgtttttgca tattcttttg ctagtcaagg agctgtacaa tgtggatttt gtattccagg
     8041 tatggtaatt agttcaaaag cacttataga cgttaataat aatcctactg aagatgatat
     8101 aaagaaagct ttaaaaggca atatatgtag atgtacaggg tatgtaaaaa taattaaagc
     8161 tgtcgattta gcggcaaaga tttttagaga aaatatagat gtgccaaaag taaactgtaa
     8221 gggtttagta ggtgaaaatc ttcctagagt tgatgcagtt ggcaaagcac ttggatattc
     8281 tgaatatgta gatgatatga gaattgaggg tatgatttat ggttcagcag ttagatctaa
     8341 gtatcctaga gctttagtta aaaaaataga tatagaagaa gcaaaagctt atccaggagt
     8401 tgttgcagtt ctaacagcta aagatattcc aggaactata aaggtaggac acttaaagaa
     8461 ggattgggat actttaatac cagaaggtga aataactaga tacttaggag atggaatagt
     8521 tttagtagca gcagaaacta gagaagcgtt agaagaggcc aaaaagctag ttaagataga
     8581 ttatgaggaa ttaacaccat taagtactac agcagatgca cttagagaag atgctccaaa
     8641 gatacatgag acagggaata tacttgttaa ggaacattta gtaagaggaa acgctaaaga
     8701 aaagatagaa aattcaaaat atgttgtaac taaaagatat actacacctg ctacagaaca
     8761 cgcattttta gagcctgaat gtgcagtagc tggtccttat gaagatgatg gagtaataat
     8821 ctattcaaca gaccaagggg tgtttgccac tcaacatgaa tgtgcagata tgttaggatt
     8881 accatatgag aaggtaagag ttataaacaa aatggtagga ggagggtttg gtggaaaaga
     8941 agacatgagt gttcaacacc atgcagcctt acttgcatac catacaaaga gatttgtaaa
     9001 agtacagtta acaagaaaag aaagtattat tgttcatcca aaacgacatg ccacagaaat
     9061 ggaatttacg acagcatgtg atgaaaatgg atacttaacg ggtatgaagg caacgataat
     9121 atctgacaca ggtgcatatg cctctcttgg aggaccagtg ctacaaagac tgtgtactca
     9181 tgcagcagga ccatacaatt atcaagatat tgatataaca ggaacagcag tatatactaa
     9241 taaccctcct ggaggagcat ttagaggatt tggagttaca cagagttgtt ttgcaactga
     9301 atgtaatcta aatttacttg ctgaaatggt tggaatatct ccttgggaga taagatataa
     9361 aaatgcaata agacctgggc aagttttacc aaatggtcaa tttgcagatg aaggaacagc
     9421 aatggcagag acactagaag ctgtaaaaga agagtatgaa aagcatccac gtgcaggtat
     9481 agcttgtgct atgaaaaata gtggggttgg agttggtata cctgatgttg gaagatgtag
     9541 attggtgata aaagatggca aagttcacat aagaacaagt gcttcttgta taggtcaagg
     9601 gcttggtact gttatgactc aagtagtttg tgaaactgct aacctacttc ctgaaaatat
     9661 agttcatgaa gttccagata ctcatttaac accagactct ggtaatacta cggcttcaag
     9721 acagacagta tttacgggag aagctactaa ggttgcatca aataagttaa aagaagcttt
     9781 agactctggc aaaactttac ttgatttaga gggagaagag ttttatggag aatatgaaag
     9841 tattacagat aaaatgggtt gtgacaaaga aaatccagta agtcatgttg cttatggata
     9901 tgcaacacaa gttgtaattc tggatgaaaa tggaaaacta gaaaaagtag tagcagcaca
     9961 tgatgttgga agagctataa atccaatcag tttagaaggt caaatagaag gtggagtagt
    10021 tatgggtctt ggatatgctc taaccgaaga ttacccagta gtcaactctg ttcctacagc
    10081 taagtttggt acattaggtt tatttaaatc tacagatata ccagaaatac aagctttaat
    10141 agttgaaaag aacaaagatt tattggctta tggggcaaaa ggtgtaggag aaatttgtac
    10201 agttcctact gcaccagcag tacaaaatgc ctattatgta tttgataaag aatttagaac
    10261 tgagttacca ctacaaaata ctccatatag taaaaaaaag taacaaaaaa aagaaatatt
    10321 aaataaaaaa atagatgaaa ttgtaaataa aaaaaaccct tttgcataag gtatatttta
    10381 aatgatagaa tatacatata caggagggtt tttcttttat aaaataagtt taataaaatt
    10441 gagaggtgag cagaatgaat tttaataata gtctagaatt aactcagtct cagaagctaa
    10501 taatgactac acaacttaag caatcattat caatattaaa tatgagtaag ttagagttag
    10561 aagaagagat aaaaagagaa gcagagaata acccattagt tgaagtcgag aaaagtgggg
    10621 agataaattg ggaagagtat ataaaagaca tggataggtc aagacgttta gatagaacag
    10681 agataagcta taatcctgat aatgaagtta atctagaaaa tttagttaaa tattcgtcaa
    10741 atttatatga agatttaaaa tttcagataa gcctgtataa acttacagac aaagaattgg
    10801 aagtctgtga gtatataatt gatagtttag atgaggatgg atatttaaga actgaggaaa
    10861 aagtaattgt tgatacctta aagatagatg aagaactttt tgaaaaatgt ttaattagtg
    10921 ttcagcagtt ggaacctagt ggagtagggg caagaagtct ttcagaatgt ctgatgatac
    10981 aaatggctag tttaggaata tataatgaaa ttttagagga aatcgttaca aaagacttaa
    11041 atttaattgc aaataataaa tataaagaga taagtaaaaa gtacaatatg tcattacaaa
    11101 aatgtgttga actaataaac ataattaaga cattagaccc caaaccagga agaacttgtt
    11161 cagtagagaa aagtgtatac attcaacctg atgtaacagt agaaaaaatt gatgatgaat
    11221 ttattgtata tttaaatgag aaagattctt atcaaataag aatcaatagt tattataaag
    11281 acatattaaa aaattcacag tctgatgaaa gtgcaaaaga atttataaaa gaaagactaa
    11341 attcagcaac aggacttatg aaaaatatag agagtaggaa aactacagta cttaagattg
    11401 cagaagagat agtaaaagct caagatgaat ttttgagaaa agggacaaaa tatataaaac
    11461 ctttgaaaat gaaagacata gcagaaaaat tagaatttca tgaatctact ataagtagag
    11521 gtgtaaatgg taaatatatg ctaacacctt ttggcgtata tgaatttagg tactttttca
    11581 gtagcgccat tgagactgag aataatgaaa tggcatctag tacaagcata aaaaaaatta
    11641 taaaggaaac aataaaagac gaaaataaaa agaaaccttt aagtgatgac catatatcta
    11701 agttactaaa agaaaaagga atcaatgttg caagaaggac agttgccaaa tatcgtgaag
    11761 aattaggaat cccatcttca agtaagagaa aagaatacta ataagtaaaa gacaaatatc
    11821 agtatggtaa ggggaaaagg ctaattaaga gatgaatgta gattgggcta aaaattttat
    11881 aaagtaaaat aatataatat gaaaaagtat taaaatgcta taaggtatta aaaaactggg
    11941 gaacattaaa acattaagat agcaaagaac ataaaaaact aaagaagatt aaaacattaa
    12001 agaacattag agctttgagg aatattagaa ctttaaagaa tattaaaatt tataaaatat
    12061 aaaaatacta aaaagaattg aaaaactggg gaacattaaa atattgtaaa atgctaaaaa
    12121 actagaaaac ataattaaat aagtgcttat aaagaatgat taaattaaat aagtgtttgt
    12181 aaagaataat tagatattag aaaaagaaga atacctagat aaataaacta ttattaaatt
    12241 taataatcac aaaaggaaaa cacgtagggt ataaataaaa atcttaacta gataaaagta
    12301 aacttagttg tagataaaaa taactagaaa taaaagtaac tagaaataaa attatttata
    12361 gtaataaata ttagaatgaa aatataaaaa aatatatata aaaagcctta tataaaaaga
    12421 aaaaatactg tgatttgaaa atggtggaga aatattttat aaaataaaaa aataaaaaag
    12481 tttttgaaaa actattgaaa tctatacgca aatcctataa aataaaaata tcttgtggga
    12541 cttaaataaa cactagggac aaaaaaagtc ccgaaaggaa agcaataagg agtatgttat
    12601 gaagaactta ttagaaattc aaaagaaatt gattccacag gcaattgaac ttatggaaag
    12661 aagatactct attttaagac aaatatcttt aagtgaacca ataggtagga gaacattatc
    12721 aaatgtatta gagattagtg aaagaataat caggtcagaa actgagtttt taaaagaaca
    12781 aggacttata gacgtagctg tatctgggat gactatcact aaagaaggtt ctgatttgtt
    12841 agataaactt aaggacatca tgagtgacat catgggattg tctaagttac aagatagagt
    12901 cagagaaaag cttggaataa aaagggtaat attagttcct ggaagctgtg atgaaaatgg
    12961 aagtttacta aaagatgtaa ctagatatgg atgcgaatat ttcttaggca ttttaaaaga
    13021 tggagatatc gtttcaataa caggaggaag cactatgttg gagttttcaa acgttattaa
    13081 aactgataag agatatccaa actctacaat agttccagct agaggaagta tgggaactga
    13141 tgtagagatg caatctaaca atatagttgc ttcaataagt aagaaattac attctaattg
    13201 caaactttta catataccag atgagttgga tgaaagtgca atgaaaactc ttagccaagt
    13261 gccagagatt aagacaactt tggagtatat taaaagaaca gacattttag tttttagtat
    13321 aggtagagca gatgtcatgg caaagagaag aaaacttcca gaagacaagg ttgatgagat
    13381 attatctaaa aatgcagttg gagaagcatt tggatattat ttcaacaaag atggagaaat
    13441 agtttacaaa ctgaacactg ttggaattga tttagaaaca gttaaacaag taaaagaaac
    13501 tattgcaatc tttgcaggga ctgaaaaagt agaagcctta ttagctatat caaatatgca
    13561 taaaaacatg gttcttgtaa cagatgaaga aagtgcttat aagatattgt cattattata
    13621 aaagtcaaga cctagcacag agctagaaaa catacattta ctttatagga ggtagcgaaa
    13681 atggttaaag tagctataaa tggatttgga agaataggaa gattagcatt aagaaaaatg
    13741 atggagcaac aagataagtt cgaagttgtt gcaataaacg acttaacaga cgctaaaatg
    13801 ttagctcatt tatttaaata tgatacagct caaggaagat ttaacggaga aatagaagtt
    13861 aaagaaggtg cttttgtagt aaacggaaaa gaaataaaag taacagcaga aagaaatcct
    13921 gctgacctac catgggctga attaggagta gatatagtat tagaatgtac aggattcttt
    13981 acatctaaag ataaagctga agctcatata caagcagggg ctaagaaagt tgttatatct
    14041 gcaccagcta ctggagattt aaaaacaata gtattcaaca caaacagtga catattagat
    14101 ggttctgaaa cagttatatc aggagcttct tgtactacta actgtttagc tccaatggct
    14161 aaagtattaa atgataaata cggtatagaa aaaggattaa tgactactat acatgcatac
    14221 actaatgacc aaaatacttt agatggtcct catccaaaag gtgatttaag aagagctaga
    14281 gctgctgctg gaaacatagt tccaaacact acaggagctg caaaagcaat aggcttagta
    14341 ataccttcat taaaaggaaa attagatggg gcagcacaaa gagtaccagt tgtaactggt
    14401 tcaataactg aattagtttg tacattagga aaaaatgtta cagttgaaga aataaatgca
    14461 gctatgaaag aagcttcaaa cgaatctttc ggatatactg aagaaatgtt agtatcttct
    14521 gacataatcg gaataagcta cggttcatta tttgacgcta ctcaaacaaa agttatggaa
    14581 gtagatggaa aacaattagt taaagttgtt tcttggtatg ataatgaaat gtcttatact
    14641 tctcaattaa taagaacttt aggatacttt gcacaattag caaaatagtc agttttgaga
    14701 aaatagaaaa gtagattgac aaagtccgag tttgtagttt aatactacgg actcaggact
    14761 ttttttaaga aaaaatatat aaaatacacc taaataatgt tacatactat atataaaaat
    14821 atgataatat gatatattta atagggtaaa agtatgtact atattatact tggcaataaa
    14881 aaattacttt attaaaagga gagatattta tgtcaatgct taacaaaaaa actatagaag
    14941 atattgatgt gtgcggaaaa aaagtattag taagatgtga ctttaatgtg ccattacaag
    15001 atggtgtaat tactgatgaa aatagattga acggagcttt accaactatt caatatttaa
    15061 tttctaaagg ggcaaaagtt atattatgct ctcacttagg aaagccaaaa ggagaagcta
    15121 aaccagaatt atctttagca ccagtagcaa agagattatc tgagatgtta ggaaaagaag
    15181 ttgtatttgc agcagacgat aatgttgtag gagaaaatgc aaagaaagca actgaaaaaa
    15241 tggaaaacgg agatgtagta ttattagaaa atactagata tagaaaagaa gaaactaaaa
    15301 acgaagagaa tttctctaaa gaactagcat cacttgctga aatatttgta aatgatgcat
    15361 ttggaactgc tcatagagca cattgctcaa cagttggtgc aggcgagttt ttacaagaga
    15421 gagtttgtgg atacttaatt caaaaagaat taaaattctt aggagaagct gttgcaaacc
    15481 ctgtaagacc atttacagct atacttggag gagctaaagt ttctgataaa ttagctgtta
    15541 taaacgaatt attagaaaaa gttgataatc taataatagg tggaggaatg gcttatacat
    15601 tcttaaaagc tcagggttat gaagtaggaa cttctttact tgaaatagat aaagttgaat
    15661 atgctaaaga aatgatggaa aaagctaaga ataagggtgt taacttatta ttaccagtag
    15721 acgttgttat ggcagaccac tttgcaccag atgctactcc aatagtaaca gaagatgcta
    15781 atgtaaaaga agattacatg ggactagata tgggacctaa gactatagca aactttgtaa
    15841 aaactataaa agaatcaaaa actgtagtat ggaatgggcc aatgggagta tttgaatttg
    15901 aaaactttgc aaatggtaca ttatcagtag ctagagctat ggcagagttg actgatgcaa
    15961 ctactgtaat tggcggtgga gatagtgcag cagcagttaa tcaattagga tttggtgaca
    16021 agatgactca cgtatcaaca ggtggaggag catcactaga atttttagaa ggtaaagaat
    16081 taccaggtat tgcagcatta gataataaat aaactaatat atagaggaga ttgtaaaatg
    16141 agaaaaccta taattgcagg aaactggaaa atgcataaaa caataaaaga agctttagaa
    16201 tttgtaaatg aagttaaaga taaagtaaat tctgataaag tagaagctgt tatatgtgca
    16261 ccatttactt tattaaagga tttaaaagaa gctactaagg gtacaaatat aaagataggt
    16321 gctcaaaata tgcactttga agaaaaagga gcatttacag gagaagtttc acctcttatg
    16381 ttaaaagaaa tagatatgga ctatgttgta ataggacatt cagaaagaag acaatacttc
    16441 aatgaaactg atgaaactgt aaacaagaaa gttttaaaag cattagaagt aggaatagac
    16501 ccaatattat gtgttggaga aactttagaa caaagagaag ctggaaaaac taaagatgtt
    16561 tgtaaagttc aagttgaaaa agctttagaa aatgttctta aagatgattt agcaaaagta
    16621 gttgttgcat atgagccaat ctgggctatt ggaactggta aaactgcaac tgctgaagat
    16681 gctaatgacg ttatatctta tataagagaa gttataaaag gattatatgg agaattagct
    16741 aatgaagtta gaatacaata cggtggaagt gttaaacctt caaatgtagc cgaaataatg
    16801 ggtcaaagtg atatagatgg agctttagta ggtggagcta gtttagctag taatgattac
    16861 ctagaccttg ttaatttcta aggagtgaaa tgatgaaaaa accagttgct ttaattatta
    16921 tggacgggtt tggatacaat aaggatgtta agggaaatgc aattgctgag tccaaaactc
    16981 ctaatttaga tagaataaaa aaagagtatc caaacacatt gataaatgct agtggtcttg
    17041 atgttggtct acctgatggt caaatgggaa attcagaagt tggacacact aacataggtg
    17101 caggtagaat agtatatcaa gatttaacta gaataacaaa atctataaaa gatggtgact
    17161 tttttactaa taaagtttta tgtgaagcaa tggataatgc aaaagaaaat tctcttcatg
    17221 taatgggact tttgtctgat ggaggagtac attcacatat agaccatctt aaggctataa
    17281 tcaaaatggc aaaagataaa ggcgtacaaa aggtgtatgt tcacgcattt actgatggta
    17341 gagatactga ccctcaaagt gcattagaat atgccaaaga agtacaagct agtatggatg
    17401 aaattggagt aggtgaattt gctacagttt ctggtagata ctacgctatg gatagagata
    17461 agagatggga aagagtagaa cttgcataca atgccatggt tagaggtatt ggtgaaaaag
    17521 caaattctat tgaggaagct attcaaaact cttatgatga tggaaaaaat gatgagttta
    17581 taatgccaac agttattatg aaagatgata aaccagctgg aagtataaag gaaaatgatt
    17641 caattatatt cttcaacttt agaccagata gagctagaca aataactaga gctttagttt
    17701 gtgaagagtt tgatggattt aaaagagaag atattaaaaa cttctttgta tgcttaacag
    17761 agtacgatat aactatagag aatgtgcata tagcatttgg acctcaatct ttagccaaca
    17821 ctctaggtga gtatctagct aaaaatggaa aaactcaact tagagctgct gaaactgaaa
    17881 agtatgcaca tgtaactttc ttcttcaatg gtggagtaga agagccaaat aaaggtgaag
    17941 aaagactatt gataccttct ccaaaggttg caacttatga tttaaaacca gaaatgtcag
    18001 catatgaatt gacagataaa gctttagata aattaggaga agataagttt gactttatag
    18061 tattaaactt tgcaaatcct gatatggtag gtcatacagg aagtatagaa gcggctataa
    18121 aggctgttga aactgtagat acttgtgtag gaaaattgat agataaaata gtagagttgg
    18181 gtggaagtgc aataataact gctgaccacg gaaatgctga atatatgtta gacccagaaa
    18241 caggaaaaac tgtaacggct cactctatta atccagtacc atttattgta gttggtcaag
    18301 aatatgaaag tgcaaaatta ttggatggag gaagattatc tgatatagca ccgactgttt
    18361 tagatatgat gaagttagaa aagccagaag aaatgactgg tcattcactt atttcaaaat
    18421 agtcgatttt gataagtaga gaataatata taaactacaa tataaattta aaaataatgc
    18481 agtttaggtt tattttgtaa agtttagact ttatgacata agatttaaat tttgtaagtt
    18541 c
//
LOCUS       DAELLV010000054        18276 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.054, whole genome shotgun sequence.
ACCESSION   DAELLV010000054 DAELLV010000000
VERSION     DAELLV010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18276
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.054"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            235..1665
                     /locus_tag="KPD58_001541"
     CDS             235..1665
                     /locus_tag="KPD58_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBG0265318.1"
                     /translation="MDIKNKIKSIFLPEKNDYLDDEEFEYDIENNEEDLKEDNKVHLN
                     DLSRVKYDYRELKDIENQTEEICLLAVKQDGTIIEFVKDKTYKVCMEAVKQTYKSLKY
                     ITNQNEDICIEAVKQNYRALYYINNKTENVLIEAIKNASTYDVMEVFKFVEEQTEDVC
                     LAFIERASKNDVAEILKGIKEQTPAICLEAVKKDGKSLAYVKEQSNSICLEAVKENYS
                     ALSCVKEQTEEICIEAVKQNDFALYYVNEQTEKICMEAVKRSYMALQYVNKQTEEICL
                     EAVRIDGRALQYVKEQTEEICLESVRQNGKVLQYVKKQTENICIEAVRGSFEELEIKE
                     ILSYVKIPTERIFVEAVKQNGKILKYVENQTELICLEAVRENYNALAYVKEQTEKICL
                     EAVNQSYEALKYVKEQTEEVCLKAVKQDYRMLKYVNNQTEKICLEAVKQNYRALEFVD
                     NQTEKVCLEAVKQNRKALQYVKQKQS"
     gene            2032..2610
                     /locus_tag="KPD58_001542"
     CDS             2032..2610
                     /locus_tag="KPD58_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBG0265319.1"
                     /translation="MFKRYAEKMTSVLICNNMIDNNESKVYSYGFEILIAFIVNITTM
                     LFIGFLFGKFTYVLFFLMCYCPIRQFSGGYHADNYFRCLLTFIFIILSTILIIENINI
                     DLFKNIIMIIASVSWVGICVLCPIEHRSNPISDREKLVYKKTAIFISTVVLLITILSL
                     SLSIFVDYFTYSAFAMFWIFVMLVLGKLKAKV"
     gene            2640..2786
                     /locus_tag="KPD58_001543"
     CDS             2640..2786
                     /locus_tag="KPD58_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HBG0265320.1"
                     /translation="MKKFIVRFMKFASSLALSTAILSANSTCPWIIHQPKVPKEISNL
                     KKTN"
     gene            3091..4470
                     /gene="scfB"
                     /locus_tag="KPD58_001544"
     CDS             3091..4470
                     /gene="scfB"
                     /locus_tag="KPD58_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HBG0265321.1"
                     /translation="MSMIHKFSMNGYNIVLDVNGGAVHVLDDVAYDLLDFYKEKSKEE
                     ILEILKSKYQEEKINEAYEEILNLEKEGLLYTEDTYQYHPSFVHREPVVKALCLNVAH
                     DCNLKCKYCFAAQGDFGGEKELMSFEVGKAAIDYLIANSGSRKNLEIDFFGGEPLMNF
                     EVVKQLVDYGRSVEKDYNKNIRFTITTNGVLLNDEIIDYINENMHNVVLSLDGRKEVN
                     DNMRPTLNDKGSYDITLPRFKKLVEKRAKDKYYYIRGTFTRDNLDFSKDVMHFADLGF
                     KLTSVEPVVGDESNPYALREEDLPKIFEEYEKFAVEYADRQLQGDGFKFFHFMIDLNQ
                     GPCVIKRITGCGAGNEYLSVTPNGDIYPCHQFVGNEEFKMANIFDEEIVLPENLKNMF
                     REAHVYTKEECKQCWNKFYCSGGCHANAINFNNDISKPYELGCEMQRKRTECSIMIQA
                     KLMLEGATN"
     gene            4499..4996
                     /locus_tag="KPD58_001545"
     CDS             4499..4996
                     /locus_tag="KPD58_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBG0265322.1"
                     /translation="MIRDYLEDKPLIDESVFVAKSADVIGNVKIGKDSSIWYNAVVRG
                     DEGPITIGENTNIQDCSIVHGDTETIIGNNVTVGHRSIVHGCKISDNVLIGMGSIILD
                     NAEIGEYTLIGAGTLITSNKKFPPGVLIMGSPGKVVRELTEEDKKYIDESYEWYLEAA
                     QNQKY"
     gene            5294..6958
                     /locus_tag="KPD58_001546"
     CDS             5294..6958
                     /locus_tag="KPD58_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HBG0265323.1"
                     /translation="MRISYRNLKRYREIGYVLIKYGFSFIVERLNIEGIAYKIPLFDP
                     PEEIKNMTTGERMKRVLEELGPTYIKIGQILSTRKDLLDQDIIDEISKLRDDVEKFDS
                     NIAIDIFKEEVGLSIEEIFLEFKEEPIAAASIGQVYEGVLKTGEEVIVKIQRPNIEKI
                     IKSDLEILRTIANTLKDLKKDFNLDLVQMIEEFQTQLMRELDYTFEAINATKFSRIFK
                     NSDEVYIPKVYSEYNTKKILVMEKVNGTKLSDVEKIRRLGYNTKTIVEIGVRSFFTQV
                     LSHGFFHADPHPGNIFVIAKNKIAYIDFGMIGIIDNKTLNQLNEIALAGVEKNVDKII
                     YLLIEMDALNGEADIKGLRQDLLYLIHYYYDISIEKINVTDILNELFRFFRQYKIVMP
                     AQFVTLAKTVITLEGTSRTLNTDFSFGSMGKEFMKHHYKSKFNPKNVVLSSRQNVEEI
                     LLDIKTIPKQLKAILKNIERNNIKMQIEDVKMTRLENCIIELTSQISLSLVLASIIVG
                     SSLIIASPNIENNIWIKFTAIAGFFISFIIGLCLVIRSIRSKYKKD"
     gene            6971..9421
                     /gene="recJ"
                     /locus_tag="KPD58_001547"
     CDS             6971..9421
                     /gene="recJ"
                     /locus_tag="KPD58_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBG0265324.1"
                     /translation="MKKWILKYKGKIEESEFSKKLNISPEICQILKNRGICTEKDSEI
                     FMNPSLDYLRDPFLMKDMQKAVDRIKLAVEKNEKIWIYGDYDVDGVSSTSILCLYFKS
                     IGYNVDYYIPNRLEEGYGINEDAIKLISSRGCDLIISVDCGITSVSEVNTANDLGIDV
                     IITDHHECQSEIPSAFAVINPKQEDCNYPFDSLCGCGVAFKMIQALTPKEEFKTSMYN
                     YLEIVTLATICDIVPLIDENRIIVKNGLKSMKEGKNIGLRELIKVCGVESDKIGSSHI
                     GFAIGPRINASGRLGYSYLGVELFTTQSQEEAVEIASILEEKNNERQMIEAKMYHEAE
                     EMLKSNSRYNDDKVLVLAKEGWQHGIIGIVASKLTEKYYKPTILLGIENGEATGSARS
                     IKGFNIFEALIKCKDLMTKFGGHEQAAGLSLDSDNVEILANEINKFADYNLTEDDMIE
                     NVNVEFELQENVINLNLVEELHKLEPFGLNNPNPRFIVRNYILKDLKVIGKNQQHLKL
                     SIEKEKSYECIGFNMSHLKSMYKVGDKVDVLFQLDENNYMGNRKVQFLLKDIRLARPK
                     SASNDKLSLKLMSKIIPKDTQSLYNISVSDFELFDGNTDINIFDYFEKDTLIISNSIN
                     GFYRAMSDISLIDLDFNINYNIIEDVSKNTDKLELIFSPNIDKIDLKRYNNIILYDYL
                     YNKGEYSYIYENKREESEIIKYYNKTDLLYLKNVVSNIVPSRDEFITIYKQALIKKEI
                     DLDMVNIRETFNVIPLKFFTILNVFRELNLLDFNLNYEKNSVLIRILPKPQKKLDLNE
                     SLILNNLKNLEKQYNSSY"
     gene            9434..9946
                     /locus_tag="KPD58_001548"
     CDS             9434..9946
                     /locus_tag="KPD58_001548"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBG0265325.1"
                     /translation="MDLKNFIRNIDDFPKPGIDFKDVTTLFKDGDAFKYAVDSIVEEL
                     KDKDVDLVIGPEARGFLMGTPVAYALGVGFVPIRKPGKLPGEVESYEYGLEYGTDTLE
                     IHKDAIKKGQKVAIVDDLLATGGTMEAAAKLVEKLGGEVVSMQFLIELKFLNGREKLS
                     NYDVNSLIKY"
     gene            10020..12227
                     /locus_tag="KPD58_001549"
     CDS             10020..12227
                     /locus_tag="KPD58_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003229747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HBG0265326.1"
                     /translation="MHDKELQEIIEKIKVYAPNANFEMVEKAYNLAKKAHEGQYRKSG
                     EPYFIHPLAVANILIEMELDIETITAGLLHDVVEDTEYTYEDIKKEFGKEVADLVDGV
                     TKLGQIKYRSKEETQSENLRKMFLAMAKDIRVILIKLADRLHNMRTLKYMPPEKAKYK
                     AKETLEIYGGIAHRLGISKIKWELEDRALRFMDPEGYYDLVSRVSMKRSQREDYIQGI
                     VELLKDKFKDLSIPCDVYGRPKHFYSIYRKMQKKHKTFEEIFDLTAVRILVDNVKDCY
                     AVLGIVHTLWRPIPGRFKDYIAMPKPNMYQSLHTTVVGPDGEPLEIQIRTHEMHNIAE
                     NGIAAHWKYKEGISSKESKVEEKLQWLRQMMEWEKDLKDPQEFMDALKEDVFNSQVYV
                     FTPKGDVIELPAESTPIDFAYRVHTNVGNKCVGAKIDGRIVPIDYKLQNGNIVEVLTS
                     SNSNGPSRDWLNIVKTPNAKSRIRQFFKKERREENVDRGNTILEREFKKHDIPQKDPL
                     VEKYMALVAKKFNQPSVEDLIATVGYGGIMSSQVVSKVKDLYLKEVKKAKKEQQIQEE
                     DLNKHNISETEYSKKRKKNASQGIIVKGLDNILVRFAKCCNPLPGDEIVGYITKGRGV
                     AIHRADCPNANNNGDFFQNRLVEVSWSSAGKGKFEAEIQIKAIDRRGIVNEITHIVAM
                     DKLSLNGINARKGKDSIVSINLLVEVNDTEELKILMKKLKAIPGVESIYRMTN"
     gene            12252..12701
                     /locus_tag="KPD58_001550"
     CDS             12252..12701
                     /locus_tag="KPD58_001550"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBG0265327.1"
                     /translation="MRAVVQRVSSSKVTVDENTIGQINKGLLVLLGVTHDDKSSDVDY
                     MIDKILNLRIFEDENDKMNLSLMDIGGELLVVSQFTLYGDCRKGRRPGFSNAARPELA
                     NNLYEEFVKKAKDKGVTVGTGQFAAHMMVELTNDGPVTILLDSSKSF"
     gene            12716..13330
                     /locus_tag="KPD58_001551"
     CDS             12716..13330
                     /locus_tag="KPD58_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0265328.1"
                     /translation="MIIKKFVDSYFGVNTYVLGDEKTKKCAVIDPGGSLVETLSFVKE
                     NELNIEYIILTHGHGDHIGYVKDIKEKTGAKVVAHVDEKELLNDKNKNLSYTMRCGAQ
                     EFDADIYVNDKDKLELGELKMTFLHTPGHTQGCMCIRVEDEMFTGDTLFAGSIGRTDL
                     YSGDFNQMEKSLKKLCKYEDAVRVYPGHGPTSTLGVEKKTNPYM"
     gene            13421..14977
                     /locus_tag="KPD58_001552"
     CDS             13421..14977
                     /locus_tag="KPD58_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HBG0265329.1"
                     /translation="MLYVFLKGHDYKYEVAELIKLFTSEFKFKDSNANNNIESKHLIN
                     RLIYENGVLFSSTEYYENGNLKYESIQNIENMNLEFLDGFDSSKTIEEFLNSLDSESK
                     RNFKKLCKENIKKSMFVVLKQVFNSYVPWGILTGIRPVKIVHNLMDKKLDDNSIRTIL
                     KDNYFIIDEKIDLALEIAKRERLFMYPIDKNKISLYVSIPFCPTRCVYCSFPSNSLKQ
                     FGHLKREYVYKLIEEIKGFAKVIKETKKEVETLYIGGGTPTTLDEEELDLLINSLFNE
                     LDLSKIKEFTVEAGRPDTITEQKLRVLKKHNVSRISINPQTMNDDTLVKIGREHRVSD
                     LVDCFNMARRMGFDNINMDIILGLIDEDLNMVKNTLEEIKKLSPESLTVHTLAIKRAS
                     NLNINMDRYKEHLTQYEEMVKMIDLSMKYAKDMGLNPYYMYRQKHMLGNLENIGYAKE
                     GYECIYNIQIMEEKQSNYALGAGAISKFVYVDEDRIERVENVKNVEQYIERVDEMIKR
                     KKEEVYKNVD"
     gene            14967..16229
                     /locus_tag="KPD58_001553"
     CDS             14967..16229
                     /locus_tag="KPD58_001553"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBG0265330.1"
                     /translation="MLTKAPRGTKDITPKEAYKWRYVENKFREICALYGYEEMVTPIF
                     EHTELFKRSVGDTTDIVQKEMYSFKDKGDREITLKPEGTAGVVRAFIENKLYADTQPT
                     KLFYVTPCFRYERPQAGRQRQFHQFGIEALGSDTPSMDAEIIALAVQFFNEVGLNDLV
                     VSINSVGCPVCRKEYNALLKEYLDSKADILCDTCNERREKNPMRVIDCKNPTCKENIK
                     DIPFIADHLCDDCKSHFDKLQEYLKEMNINFVIDKTIVRGLDYYRKTAFEIISNDIGA
                     QSTVCGGGRYDGLVEQLGGPKGISGIGFGLGIERLLLTLEGNGIEIENPQSTDIFIVT
                     IGEKANTRSFKLLKDLRQNHISADKDHIERSVKAQFKYSDKINSKFTIVIGDDELKND
                     TATLKNMKTSEQTTVKLSTLVEELKQKL"
     gene            16303..18090
                     /gene="aspS"
                     /locus_tag="KPD58_001554"
     CDS             16303..18090
                     /gene="aspS"
                     /locus_tag="KPD58_001554"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBG0265331.1"
                     /translation="METLKGLKRTHYCGELREKNINEEVVLMGWVQKKRNLGGLVFVD
                     LRDTSGLCQIVFDTDVDKEAFEKAEKLGAEFVIAVKGKVCERQSKNPNMPTGDIEIFA
                     TELRLLNKSETPPIYIKDDDDVSEALRLKYRYLDLRKPSMQRNLKLRHKVMNITRNYL
                     SNNRFCEIETPFLIAPTPEGARDYLVPSRVNPGKFYALPQSPQLYKQLLMVSGMDRYF
                     QIVKCFRDEDLRADRQPEFTQIDCEMSFVEQEDVMSMIEGLLEAIFKEVLDVELALPL
                     PKMTYAEAMSKYGSDKPDTRFGYELTDISDVVCNCGFKVFADATQPGKSVRGINVKGK
                     ADDFTRKQISSLEEHAKTYRAKGLAWMKVGQEGVTSPIAKFFNEEEMNAILTRMNAEV
                     GDLLLFVADKNSIVFDALGQVRLEVANRLNLLDKNVYNLLWVTEFPVFEEDEETGTFS
                     AMHHPFTSPMDEDLDKLEEGDKSSLRAKAYDIVLNGYEIGGGSVRISNSDVQSRMFKA
                     LGFTEERANEKFGYLLEAFKYGTPPHAGLAFGLDRLVMLLAGTDNIREVIAFPKNQNA
                     VCPMTNAPTLAEDEQLEELSIKVDIKDNE"
BASE COUNT         7448 a         1816 c         3386 g         5626 t
ORIGIN      
        1 tttgaaaaaa atcactataa atgagtaata aatataaata gtgtatacta atgggatgga
       61 aaatgtccac tattaacttt attaccccac atatataaga taaaattatt aatataaaat
      121 ataatctatc gacaatgaat agacttttaa ataaacttaa tataaaatta attaaataag
      181 tgcttaaaaa cgatttactt taatgataga atcatattaa aggaggataa ttttatggat
      241 ataaagaata aaattaaatc tatattttta ccagaaaaaa atgactattt agatgatgaa
      301 gaatttgagt atgatataga aaataatgaa gaagatttaa aggaagataa caaagtacat
      361 ttaaatgatt taagtagagt aaaatatgat tacagagagt taaaagacat agaaaaccaa
      421 acagaagaga tatgtctgct agcagtaaag caggatggta ctataattga gtttgtaaag
      481 gacaagactt ataaagtatg tatggaagca gtaaaacaga catataaatc tcttaagtat
      541 ataacaaatc aaaatgagga tatttgtata gaagcagtaa aacagaacta cagagctttg
      601 tactacataa ataataagac agaaaatgta ctaattgagg ctataaaaaa tgcatcaact
      661 tatgatgtca tggaagtatt taaatttgtg gaagagcaaa cagaagatgt atgtctagca
      721 tttatagaaa gggcatctaa aaatgatgtt gcagagatat taaaaggaat aaaagagcaa
      781 actccagcca tatgtttaga agctgtaaaa aaagatggaa aatctttagc ttatgtaaag
      841 gaacaaagta atagtatatg tttagaagct gtaaaagaaa actacagtgc actatcttgt
      901 gtaaaagagc aaactgaaga aatatgtata gaagcagtaa aacagaatga ctttgcattg
      961 tattatgtaa atgaacagac agaaaaaata tgtatggaag cagtaaaaag aagttatatg
     1021 gctctacagt atgtaaataa gcaaacagaa gagatttgtt tggaagcagt aagaattgat
     1081 ggaagagcat tacagtacgt aaaagagcaa acagaagaaa tttgcttgga gtcagttaga
     1141 caaaatggaa aagtactgca atatgtaaaa aaacagactg aaaatatatg tatagaagca
     1201 gtaagaggtt catttgaaga acttgaaata aaagaaatat taagttatgt aaagatacca
     1261 acagaaagaa tatttgtaga agcagtaaag caaaatggaa aaattcttaa atatgtagaa
     1321 aatcagacag aattaatatg tttagaggca gtaagagaaa actataatgc tttagcttat
     1381 gtaaaggaac aaacagaaaa gatttgttta gaagcagtta atcaaagtta tgaagcgctt
     1441 aaatatgtga aagaacaaac agaagaagtt tgtctaaaag cagtaaaaca agattataga
     1501 atgttgaaat atgtaaataa tcaaacagaa aaaatatgtt tagaagcagt aaagcaaaat
     1561 tatagagctt tagaatttgt tgataatcaa acagaaaaag tttgtcttga agcagtaaaa
     1621 cagaatagaa aagcactaca gtatgtaaaa caaaaacaaa gttaaaaggc taaaatatat
     1681 agaaaatata tgcaaataaa taaaatttga tattttgtaa taaataaaat gggtatctca
     1741 aaaggaactt tttagtttgt gaggtactct ttttatatga aataaaatat atttttagac
     1801 ttttaacaat ggggaaaagg tttatttcta ttttgacata ggctttttat gcatataaag
     1861 cttgataaaa ttgttttatt ataattgttt tgttatataa ataataagaa gaaaattata
     1921 taggtctttt tttgaccagt tttacatttt taacaactag tttgtaaagt atattgaatt
     1981 ttctcaaaaa agaatgtact ctatatatgt aaaatttttt tggaggtaaa aatgtttaaa
     2041 agatatgctg aaaaaatgac atctgtttta atttgtaata atatgataga taataatgaa
     2101 tctaaagtct attcttatgg atttgaaata ttaatagctt tcatagttaa tataaccacc
     2161 atgctgttta taggtttttt atttggtaaa tttacatatg tattattttt tttaatgtgt
     2221 tattgtccaa taagacaatt ttcaggaggg tatcatgcag acaattattt tagatgctta
     2281 ttaaccttta tttttataat tttatccaca atcttaatta tagaaaatat aaatatagac
     2341 ttatttaaaa atattattat gattattgca agtgttagtt gggttggaat ttgtgttctg
     2401 tgcccaatag aacataggag taaccctata agtgataggg aaaagttggt ttataagaaa
     2461 actgctatat ttatatctac agtagtgtta ttgataacaa ttttaagtct ttctttgagt
     2521 atatttgtag attatttcac atatagcgca tttgccatgt tttggatttt tgtgatgtta
     2581 gtattaggta agttaaaagc taaagtataa gtaaaatatg tatgagggag agttaagtta
     2641 tgaaaaagtt tattgtaaga tttatgaagt ttgctagctc attggcactt agcactgcta
     2701 ttttgtcagc taatagtacc tgtccttgga ttattcatca gcctaaagta cccaaagaaa
     2761 tcagcaatct aaagaagaca aattaaatac aagcacttat ttatacaaaa ttatcaggaa
     2821 aaaagaaatt attatatttt aataagatta aaacaatata aaatcagata caaaatttat
     2881 tttatttatt ttataggagc ctaataccta tttggtaata tattagtgcg ccaatttagg
     2941 gggatatagt gtatttaatg aaatgactta cggagagtaa gtcatttttg tattgtatgt
     3001 attttatgta aacttatgat ataatgaagg gtaaagttta attaaaataa taaaaaactt
     3061 tagtatagat agtaatttag gaggtagagt atgagtatga tacataagtt ttctatgaat
     3121 ggatataata tcgttttaga tgtgaatggt ggagctgttc atgtgttaga tgatgttgca
     3181 tacgatttgt tagattttta taaagaaaaa agtaaagaag aaatattaga gatattaaaa
     3241 tcaaaatatc aagaagaaaa aataaatgaa gcatatgaag aaatattaaa tttggaaaaa
     3301 gagggtcttt tatatacaga agatacatat caatatcatc caagctttgt acatagagag
     3361 cctgtagtaa aagctttatg tttaaatgta gctcatgatt gtaacttaaa atgtaaatac
     3421 tgttttgcag ctcaaggtga ttttggagga gaaaaagaac ttatgagttt tgaagttggt
     3481 aaggcagcaa ttgactattt gattgctaat tcaggaagta gaaagaacct agagatagat
     3541 ttctttggtg gagagccttt gatgaacttt gaagttgtaa aacaactggt tgactatgga
     3601 agaagtgtag aaaaagatta taataaaaat ataagattta caataactac aaatggtgta
     3661 ctgttaaatg atgagattat agattatata aatgaaaata tgcataatgt agttttaagt
     3721 ttagatggaa gaaaagaagt taatgataat atgagaccaa ctttaaatga taaaggaagt
     3781 tatgatatta cattaccacg atttaagaaa cttgtggaaa aaagagcaaa agataagtat
     3841 tattatataa gaggtacttt tacaagggat aatttagatt tttcaaaaga tgttatgcat
     3901 tttgcagatt taggatttaa attaacctct gtagagccag tcgttggaga tgagagtaat
     3961 ccatatgcac ttagagaaga agatttacct aagatatttg aagagtacga aaaatttgct
     4021 gtagagtatg cagatagaca attacaagga gatggattta aatttttcca ttttatgata
     4081 gacctaaatc aaggtccatg tgtaataaag agaataactg gatgtggagc tggaaatgag
     4141 tatctttctg taactccaaa tggagatatc tatccatgtc atcaatttgt tggaaatgaa
     4201 gaatttaaaa tggcaaatat atttgatgaa gaaatagtac ttccagaaaa tttaaaaaat
     4261 atgtttagag aagctcatgt atatactaaa gaagagtgta aacaatgttg gaataagttt
     4321 tactgttcag gtggatgtca tgcaaatgcg ataaacttta ataatgacat atcaaaacca
     4381 tatgaattag gctgtgaaat gcaaagaaaa cgtacagagt gctctataat gatacaagct
     4441 aagttaatgt tagaaggagc aaccaactaa atttatattt taaaggagtt gtttatatat
     4501 gattagagat tatttagaag ataaaccttt aattgacgaa agtgtatttg tggcaaaatc
     4561 agcagatgta attggaaatg ttaaaatagg caaagattca agtatatggt acaatgcagt
     4621 agttaggggt gatgaaggac caataactat tggagaaaac actaatatac aagattgttc
     4681 tatagtgcat ggagatacag aaactataat tggtaacaat gtaacagttg ggcataggtc
     4741 tatagttcat ggttgtaaaa ttagtgataa tgtgttaatt ggtatgggtt caataatatt
     4801 ggataatgct gaaataggag aatatactct gataggagca ggtacactaa ttacatctaa
     4861 taagaagttt ccaccaggtg ttttgataat gggttctcct ggtaaggttg ttagagagct
     4921 tacagaagaa gataaaaaat atattgatga atcttatgaa tggtatttag aggcagcaca
     4981 aaatcaaaaa tattagttta aagagtggtt gtttcaaaac atatttaaat ctgttttgag
     5041 gcaaccacta atttatttta ttagattttg ttgatatttt agttttcaaa aagtatttga
     5101 ttaaaccaga tataaaatgt caaaaataaa aattaactga gttaaataat tttaataaaa
     5161 ttggtagtat atgtgataaa attaaatatt aaagaggata taggatatta gaatatcaat
     5221 tggtgaaata atgtcatata cgataaatta aggtgtaagt ttttgggtat acctatctta
     5281 ggagtgaaaa gatttgagaa taagttatag aaatttaaag cgatatagag aaattggata
     5341 tgttttaata aagtatggtt ttagttttat tgtagaaaga ctaaatatag aaggaattgc
     5401 ctataaaata ccactatttg acccaccaga agaaataaaa aatatgacaa ctggtgagag
     5461 aatgaaaaga gttttagaag aactaggacc tacatatata aaaataggac agatattgag
     5521 tacaagaaaa gacttgttag accaggatat aattgatgaa atatcaaaac tcagagatga
     5581 tgtagagaaa tttgattcaa atatagctat tgatatattt aaagaagaag tgggtttaag
     5641 tatagaagaa atattcttag aatttaaaga agaaccaata gcagcagctt caattggtca
     5701 agtttatgaa ggtgtactta aaacgggaga agaagtaatc gtaaaaattc aaagaccaaa
     5761 tatagagaaa ataataaaat ctgatttaga gattcttaga actattgcaa atactttaaa
     5821 agatttaaaa aaggatttta atttagattt agtacagatg attgaagaat ttcaaactca
     5881 attaatgagg gaacttgatt atacatttga agctataaat gcaactaaat tctctaggat
     5941 atttaaaaat agtgatgaag tatatatacc taaagtatat tcagaatata atactaagaa
     6001 aattttagta atggaaaaag ttaatggtac aaagttaagt gatgtagaaa aaataaggag
     6061 actaggttat aacacaaaaa ctattgttga aataggcgtt aggtcatttt ttacacaggt
     6121 attatctcat ggattttttc atgcagaccc acatccaggt aatatattcg taattgctaa
     6181 aaataagata gcatatatag attttggaat gattggaatt attgataata aaactttaaa
     6241 ccaattaaat gaaattgcat tagcaggtgt agaaaagaat gttgacaaga taatatatct
     6301 attgatagaa atggatgcat taaatggaga ggctgatatt aagggactta gacaggattt
     6361 actttatcta atacattatt actatgatat ttctatagaa aaaataaatg taacagatat
     6421 attaaatgaa ctatttaggt tttttagaca gtataaaata gtcatgcctg cacaatttgt
     6481 aactcttgca aagacagtta taacattaga aggaacaagt agaactttaa acacagattt
     6541 ttcttttggt tctatgggaa aagagtttat gaaacatcat tataagagta aatttaaccc
     6601 taaaaatgtt gttttaagtt caagacaaaa tgtagaagaa atattactgg acataaaaac
     6661 tattccaaag caattaaaag caattttaaa aaatatagaa agaaataata taaaaatgca
     6721 aattgaagat gttaaaatga cgaggttaga aaattgtata atagagctaa catctcaaat
     6781 atctttgagt ttagtgttgg catctattat agtaggttct tctcttataa ttgcatctcc
     6841 aaatatagaa aataatatat ggataaaatt tactgcaata gcgggtttct ttatatcctt
     6901 tataataggc ctttgcttag ttataagaag tattaggtca aaatacaaaa aagattaggg
     6961 ggaattaaat ttgaaaaaat ggatattgaa gtataaaggt aagatagagg aaagtgaatt
     7021 tagcaaaaaa cttaatattt ccccggaaat ctgccaaatt ttaaaaaata gagggatatg
     7081 tactgaaaaa gattcagaga tatttatgaa tccatcttta gattatttaa gagacccttt
     7141 tttaatgaaa gatatgcaaa aagctgttga tagaataaag ttagcagttg agaagaacga
     7201 aaaaatttgg atttacggag attatgatgt tgatggtgta tcatctacat ctatcttgtg
     7261 cttatatttt aagagtatag gttacaatgt tgattactat attcctaata gattagaaga
     7321 agggtatggt ataaatgaag atgctattaa gcttataagc tctaggggat gtgacttgat
     7381 tataagtgtt gactgtggaa ttacatcagt gtctgaggtc aatacagcga atgatttggg
     7441 catagatgtt ataattacag accatcatga atgtcaaagt gaaattccat cagcctttgc
     7501 agtaataaat ccaaaacaag aagattgtaa ttatccattt gattcactat gtggatgtgg
     7561 tgtagcattt aaaatgattc aagccttgac tccaaaagaa gaatttaaaa ctagtatgta
     7621 taattatttg gaaatagtaa cactagcaac tatttgtgat attgttccac ttatagatga
     7681 aaatagaata attgtaaaaa atggtttaaa atcaatgaaa gaaggcaaaa atattggatt
     7741 aagggagctt ataaaagttt gtggtgtaga aagtgataag ataggttctt ctcatatagg
     7801 ttttgcaata ggtcctagga taaatgcttc aggtcgattg ggatattctt acttaggagt
     7861 agagctattt actactcaat cacaagaaga ggcagtagaa atagctagta tattggaaga
     7921 aaaaaataat gaaagacaga tgatagaagc taagatgtat catgaagctg aggaaatgct
     7981 aaagtctaat agcagataca atgatgacaa ggtgttggtt ttagctaaag agggttggca
     8041 acatggaata ataggaattg tagcatcaaa gttaactgaa aaatactaca aaccaacaat
     8101 tcttctaggt atagaaaatg gggaggctac tggttcagcg aggtcaataa aaggatttaa
     8161 tatatttgaa gctctcataa agtgcaaaga cttgatgact aagtttggtg gtcatgaaca
     8221 agcagcaggg ttatccttgg attcagacaa tgtagaaatt ttagcaaatg aaataaataa
     8281 atttgcagat tataatttaa ctgaagatga tatgatagag aatgtaaatg tcgagtttga
     8341 attacaagaa aatgtaataa atttaaatct tgtagaagaa ttacataagt tagaaccctt
     8401 tggattaaat aatcctaatc ctagatttat agttagaaat tatattttaa aagatttgaa
     8461 agttataggt aagaatcaac aacatctgaa attaagtata gaaaaagaga aaagctatga
     8521 atgtatagga tttaatatga gtcatttaaa atcaatgtat aaagtaggag acaaagtaga
     8581 tgtactattc caattagatg aaaataacta tatgggaaat aggaaagtac aatttttact
     8641 taaagatata cgattagctc gacctaaaag tgcaagtaat gacaaacttt cattgaaatt
     8701 aatgtcaaaa ataataccaa aagatactca aagcctgtat aatatatctg tgtctgattt
     8761 tgaattattt gatggaaata cagatataaa tatatttgat tattttgaaa aagatacact
     8821 aataatatct aattctataa atggatttta tagagcaatg tcagatatat cacttataga
     8881 tttggatttt aacataaatt acaatattat tgaagatgtt agcaaaaata ctgataaatt
     8941 agagcttata ttttctccta atattgataa aatagactta aaaagatata ataatattat
     9001 tctttatgat tatttataca ataaaggcga atattcgtat atatatgaga ataaaagaga
     9061 agaaagtgaa attataaaat attataataa aacagaccta ttatatttaa agaatgtagt
     9121 tagtaatata gttccatcaa gagatgaatt tataacaatt tataaacaag cactcataaa
     9181 aaaagaaatt gatttggata tggtaaatat aagggaaact tttaatgtaa ttccattaaa
     9241 gttttttacg atattaaatg tatttagaga attaaattta cttgacttta atttaaatta
     9301 tgaaaaaaat tctgtactca taagaatatt gccaaaacca cagaaaaaat tagacttaaa
     9361 tgagagttta atactcaata atctaaaaaa tttagaaaaa caatacaata gcagttatta
     9421 ggaggcgttt cccatggatt taaaaaactt tataaggaat attgatgatt tcccaaagcc
     9481 aggcatagat tttaaagatg tgacaactct atttaaagat ggagatgcat ttaaatatgc
     9541 agtggattct atagttgaag aattgaaaga taaagatgta gatttagtaa taggaccaga
     9601 agcaagagga tttttgatgg gtacacctgt agcatatgct ttaggagtag gatttgttcc
     9661 aattagaaaa cctggaaagt taccaggtga agttgagagt tatgagtatg gattagagta
     9721 tggtacagat actttagaga tacataagga tgcaattaaa aagggtcaaa aagtagcaat
     9781 agtagatgat ttattggcta ctggtggaac tatggaagcc gctgccaagc ttgtagaaaa
     9841 attaggtgga gaagtagttt ctatgcaatt tttgattgag ctaaaattct taaatgggag
     9901 agaaaagttg tctaactatg atgttaattc tcttataaaa tactagtact tttataatag
     9961 ttggtataac accaactatt ttcatatata ttaaaatttt atacaaaata ggtggttata
    10021 tgcatgataa agaattacaa gaaataattg aaaaaataaa agtttatgca cctaatgcaa
    10081 attttgaaat ggttgaaaaa gcctataatc ttgcaaaaaa agcgcatgaa ggacaatata
    10141 gaaaatctgg ggaaccatac tttattcatc cattagcagt tgcaaatatt ttaatagaga
    10201 tggaattaga tattgaaact ataacagcag gtttacttca tgatgttgta gaagatacag
    10261 aatataccta tgaagacata aaaaaagaat ttggcaaaga agtagcggat ttggttgatg
    10321 gagtaactaa acttggtcaa ataaaatatc gttcgaaaga agaaactcaa tctgaaaatt
    10381 taagaaaaat gtttttggca atggcgaagg atataagggt tatacttata aaacttgctg
    10441 atagattgca caacatgaga acattaaaat acatgcctcc agaaaaagca aagtataaag
    10501 caaaagagac acttgaaatt tatggtggta tagctcatag attaggaatt tccaagataa
    10561 agtgggaact agaagacaga gcattaagat ttatggaccc agaagggtat tatgatttag
    10621 ttagtagagt atcaatgaag agaagtcaaa gagaggatta tattcaagga attgttgaac
    10681 ttcttaaaga taagtttaaa gatttaagta taccatgtga tgtatatggt agaccaaagc
    10741 atttttacag tatatataga aagatgcaaa aaaaacataa gacttttgaa gagatttttg
    10801 atttaacagc agtaaggata ttggttgata atgtaaagga ttgttatgca gtacttggta
    10861 tagttcatac attatggaga cctattccag gaagatttaa agattatata gcaatgccaa
    10921 aacccaatat gtatcaatca ttacacacca ctgttgtagg tcctgatgga gaacctttag
    10981 agatacaaat aagaactcat gagatgcata atatagctga aaatggtata gcagcccatt
    11041 ggaaatataa agaaggtatc tctagcaaag aatcaaaggt tgaagaaaaa cttcaatggt
    11101 tgagacagat gatggagtgg gaaaaagatt taaaagaccc acaagagttt atggatgcac
    11161 ttaaagaaga tgtatttaat agtcaagttt atgtatttac gccaaaagga gacgtaattg
    11221 aattaccagc agaatcaaca cctatagatt ttgcctatag ggttcataca aatgtaggta
    11281 ataaatgtgt aggagctaaa attgatggaa gaatagttcc tattgattat aagcttcaaa
    11341 atggtaatat tgtcgaggta cttacatcat ctaattctaa tggaccaagt agggattggc
    11401 ttaatatagt gaagacccca aatgcaaaaa gtagaattag acaatttttc aagaaggaaa
    11461 gaagagaaga aaatgttgac cgtggaaata caatattaga aagagaattt aagaagcatg
    11521 atataccaca aaaagaccct cttgttgaaa aatatatggc attagttgct aagaaattta
    11581 atcagccaag tgttgaggac ttaattgcca ctgtaggtta tggtggtata atgtcatctc
    11641 aagtggtatc taaggtaaaa gacctatatt taaaagaggt taaaaaagct aaaaaggaac
    11701 aacagataca agaagaagat ttaaataaac acaatataag tgaaacagaa tacagtaaaa
    11761 agagaaagaa aaacgcatca caaggaatta tagtaaaagg gcttgacaat atcttagtaa
    11821 gatttgctaa gtgttgtaat cctcttccag gagatgaaat tgtaggatat attactaagg
    11881 gtaggggagt agccatacac agagctgatt gcccaaatgc aaacaataat ggtgatttct
    11941 tccaaaatag attggtggag gtttcatgga gttcagcagg aaaaggtaag tttgaagcgg
    12001 aaattcaaat aaaagctatt gacagaagag gaattgttaa tgaaataact catattgttg
    12061 caatggataa acttagttta aacggaataa atgcaagaaa aggtaaggat agtattgtaa
    12121 gtataaactt acttgtagag gttaatgaca cagaggaatt aaaaattcta atgaaaaaat
    12181 taaaagcaat tcctggtgta gaaagtattt atagaatgac aaattagagt taaaataata
    12241 gggaggaaac tatgagagca gtagtgcaaa gagtatcttc atctaaagtt acagtagatg
    12301 aaaacactat aggtcaaata aataaggggt tactagtatt gttaggagtg actcatgatg
    12361 ataaatcaag tgatgtagat tatatgattg ataaaatatt gaatctgaga atttttgaag
    12421 atgaaaatga caagatgaat ttatctctaa tggatatagg tggagagctt ttagtagtat
    12481 ctcaatttac tttatatgga gattgcagaa aaggaagaag acctggattt agtaatgcag
    12541 ctagaccaga acttgcaaat aacctttatg aagaatttgt aaaaaaagca aaagataaag
    12601 gtgtgacagt tggtacaggt caatttgcag ctcatatgat ggtagagtta actaatgatg
    12661 gtccagtaac aatactatta gactcaagta aatcttttta agggggaaaa ctttaatgat
    12721 tataaaaaaa tttgtagata gttattttgg agtaaatact tatgtattag gtgatgaaaa
    12781 gactaaaaaa tgtgcagtta tagacccagg tggaagttta gtagagacat taagttttgt
    12841 aaaagaaaat gaattaaata ttgaatatat tatccttact catgggcatg gagaccatat
    12901 aggatatgtt aaggatatta aagaaaaaac tggtgctaaa gtagttgcac atgttgatga
    12961 aaaagaatta ttaaatgaca aaaataaaaa tctaagttat acaatgagat gtggagcaca
    13021 agagtttgat gcagatatct atgtaaatga taaagacaag cttgaacttg gtgaattaaa
    13081 aatgactttc ttgcatactc caggacatac tcaaggatgt atgtgtataa gagttgaaga
    13141 tgaaatgttc acaggagata ctttgtttgc tggaagtatt ggtagaactg atttatatag
    13201 tggagacttt aaccaaatgg aaaaatcact taagaaatta tgtaaatatg aagatgctgt
    13261 tagagtatat cctggacatg gaccaaccag tactctaggg gttgaaaaga agacaaatcc
    13321 ttacatgtaa ttttatatta gtatttaaaa aagtataaaa tatttaataa ctatattagt
    13381 gagggggttc ttctactgtt aaatagaagg agttaagttg atgttgtatg tttttttaaa
    13441 aggacatgat tataagtatg aagtagcaga acttataaaa ttgtttacta gtgaatttaa
    13501 gtttaaagac tctaatgcta acaataatat agagtcaaag catttaataa atagattgat
    13561 ttatgagaat ggtgtattat tctcaagtac tgaatactat gaaaatggaa acttaaagta
    13621 cgagtcaata cagaatatag aaaatatgaa tcttgaattt ttagatggat ttgatagcag
    13681 caaaacgatt gaagaatttc ttaatagcct agatagtgaa agtaagagga actttaaaaa
    13741 gctgtgtaaa gaaaatatca aaaaaagcat gtttgtagtg cttaaacaag tatttaattc
    13801 ttatgtacca tggggaattt tgactggtat tagaccagtc aaaattgtcc acaatttaat
    13861 ggataaaaag ctagatgata atagcataag aaccattcta aaagataatt attttataat
    13921 agatgagaaa atagatttag ctcttgaaat agctaaaaga gagcgattat ttatgtatcc
    13981 tatagataaa aataaaattt ctctttatgt aagtattcca ttttgtccaa ctagatgtgt
    14041 atattgttca tttccttcaa attcattaaa acaatttgga catttaaaaa gagaatatgt
    14101 ttataaattg attgaagaaa ttaaggggtt tgccaaagtt attaaggaga ctaagaaaga
    14161 agttgaaact ctatatattg gtggtggaac tccaactaca cttgatgagg aagaattaga
    14221 tttgcttata aattcattgt tcaatgaatt agatttaagt aaaattaaag aatttactgt
    14281 agaagcaggt agacctgaca ctattacaga acaaaaacta agagtgttaa aaaaacacaa
    14341 tgttagtaga attagtataa atcctcaaac tatgaatgat gataccttgg taaagattgg
    14401 aagagagcat agagtatctg atttagtaga ttgctttaat atggctcgta gaatgggatt
    14461 tgataatata aatatggata taattttagg tcttatagat gaagatttaa atatggtaaa
    14521 gaatacattg gaggagataa aaaaactttc tcctgaaagc cttacagttc atacattagc
    14581 cataaagaga gcatccaact taaatataaa tatggatagg tataaagaac atctaacaca
    14641 atatgaagaa atggtaaaaa tgatagattt atcaatgaaa tatgcaaaag atatgggatt
    14701 aaatccatat tatatgtata gacaaaaaca tatgttggga aatttagaaa atataggtta
    14761 tgctaaagaa ggatatgagt gcatttataa tatacagatt atggaagaaa aacaaagtaa
    14821 ctatgcatta ggtgcaggtg ctatatcgaa atttgtttat gtagatgaag atagaattga
    14881 aagagtagaa aatgtaaaaa atgttgaaca atacattgaa agagtagatg aaatgataaa
    14941 aaggaagaaa gaagaggtat ataaaaatgt tgactaaagc tcctagagga actaaagaca
    15001 taactccaaa agaggcttac aaatggcgtt atgtagaaaa taaatttaga gaaatatgtg
    15061 ctttatatgg atatgaagaa atggtaacac ctatatttga acatacagaa cttttcaaga
    15121 gaagtgttgg agatactaca gatatagttc aaaaagaaat gtattctttt aaagataaag
    15181 gtgatagaga aattacccta aagccagaag gtacagcagg tgtagtaaga gcatttatag
    15241 aaaataaatt atatgcagat actcaaccaa caaaattatt ttatgtaaca ccttgtttta
    15301 gatatgaaag accacaagct ggtagacaaa gacaatttca ccaatttgga atagaagcac
    15361 ttggaagtga tactccttca atggatgctg aaataatcgc actagctgtt caatttttta
    15421 atgaagttgg attaaatgat ttggtagtga gtataaattc agttggctgt ccagtttgta
    15481 gaaaagagta caatgcttta ttaaaagagt atcttgactc aaaagctgat atattgtgtg
    15541 atacttgcaa tgagagaaga gaaaaaaatc caatgagggt tatagactgt aaaaatccta
    15601 catgtaaaga aaatatcaaa gatataccat ttattgcaga ccatttgtgt gatgattgta
    15661 aatctcactt tgataaactt caagaatact taaaggaaat gaatataaac tttgtaattg
    15721 ataaaacaat agttagaggc ttagattact atagaaaaac tgcttttgaa attatatcta
    15781 atgatatagg agctcaaagt actgtatgtg gtggaggaag atatgatggt cttgttgaac
    15841 aattaggagg accaaaaggt ataagtggaa ttgggtttgg actaggaatt gaaagacttt
    15901 tacttacact tgaaggtaat ggaattgaaa tagaaaaccc tcaatctacg gatatattta
    15961 tagtaactat aggtgaaaaa gcaaatacaa gaagctttaa attgttaaaa gacttgagac
    16021 aaaatcatat aagtgcagat aaagaccata tagagagaag tgttaaagca caatttaaat
    16081 attcagataa aataaattca aaatttacaa tagtaattgg agatgatgag cttaaaaatg
    16141 acactgcgac attaaaaaat atgaaaacat cagagcaaac tactgtaaaa ttaagtacat
    16201 tagttgaaga attaaagcaa aagctgtaaa ataagaaagt atgatgtttt gtgtattaga
    16261 taaatatata gcattaaaat taaataaaat ggaggtagta tcttggaaac tttaaagggt
    16321 ttaaagagaa ctcactactg tggagagttg agagaaaaaa acataaatga agaagtcgta
    16381 cttatgggat gggtacagaa aaaaagaaat ttaggtggtc ttgtatttgt tgatttaaga
    16441 gatacaagtg gattatgtca aatagttttt gatacagatg tagataaaga agcttttgaa
    16501 aaagctgaaa aattaggggc tgaatttgta atagctgtaa aaggaaaagt atgcgaaaga
    16561 caatctaaaa accctaatat gccaactggt gatatagaaa tatttgcaac tgaacttaga
    16621 cttttaaaca agtcagaaac tccgcctatt tatataaaag atgatgatga tgtttctgaa
    16681 gcactaagat taaaatatag atacttagat ttaagaaaac catctatgca aagaaattta
    16741 aaattaagac ataaagtaat gaatattaca agaaattact tatcaaacaa tagattttgc
    16801 gaaatagaaa caccattttt aatagctcca acaccagaag gagcaagaga ttatctagta
    16861 ccaagtagag ttaatccagg aaaattttac gcattaccac aatcaccaca attatataaa
    16921 caattattga tggtaagtgg tatggataga tatttccaaa tagttaaatg ctttagagat
    16981 gaagatttaa gagcagatag gcaaccagag tttacacaaa tagactgtga aatgtctttt
    17041 gttgagcaag aagatgttat gagtatgatt gaaggtttgc ttgaagcgat atttaaagaa
    17101 gttttagatg tagaattggc acttccactt cctaagatga cttatgcaga agctatgtct
    17161 aaatatggtt cagataaacc agatactaga tttggatatg agttaacaga tatatctgat
    17221 gttgtttgta actgtggatt taaagtgttt gctgatgcta cacaaccagg aaaaagtgtt
    17281 agaggtataa acgtaaaagg caaagctgac gactttacta gaaaacaaat atcatcacta
    17341 gaagaacatg ctaagacata tagagcaaaa ggtcttgctt ggatgaaagt aggacaagaa
    17401 ggagtaactt ctccaatagc aaaattcttt aatgaagaag aaatgaacgc tatacttaca
    17461 agaatgaatg ctgaagtagg agatttactt ttatttgtag ctgataaaaa ctcaatagta
    17521 tttgatgctt taggacaagt tagacttgaa gtagcaaata ggcttaatct attggataaa
    17581 aatgtatata acttactatg ggtaactgag ttcccagttt ttgaagaaga tgaagaaaca
    17641 ggcacattct cagcaatgca tcacccattt acatcaccta tggatgaaga tttagacaag
    17701 ttagaagaag gtgataagtc atctttaaga gcgaaagctt atgatattgt attgaatggt
    17761 tatgaaatag gtggaggaag tgttagaata tcaaattctg atgttcaatc aagaatgttt
    17821 aaagcacttg gttttacaga ggaaagagct aatgaaaagt ttggttattt attagaagca
    17881 tttaaatatg gtactcctcc tcatgctggt ctagcttttg gtcttgatag acttgttatg
    17941 ttacttgcag gaactgataa tataagagaa gttattgcat tccctaaaaa tcaaaatgct
    18001 gtttgcccta tgacaaatgc tcctacattg gcagaagatg agcaattaga agaattaagt
    18061 ataaaagttg atataaaaga taatgaataa tatgtagttg caagaaacta aaatgtaaaa
    18121 agtctggata taaagtatat ttatattcag acttttttta ttagaattat tttatatttt
    18181 ttctctatat attttcataa attatctttg tattctcagc caatataatt aaaatactat
    18241 gtttaaaaat tttacataaa cttgattgtt ttagaa
//
LOCUS       DAELLV010000055        17970 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.055, whole genome shotgun sequence.
ACCESSION   DAELLV010000055 DAELLV010000000
VERSION     DAELLV010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17970
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.055"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            22..1302
                     /locus_tag="KPD58_001555"
     CDS             22..1302
                     /locus_tag="KPD58_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBG0265332.1"
                     /translation="MNILGLIVEYNPFHNGHIYHLSKSIEKTNATHTVAIMSGNFLQR
                     GEPALFDKYTRAKIAVENGVDLVIELPTLFACQSAEIFSHGAITILNSLNCINSICFG
                     SEEGNTEILYNLAEILANEPNEFKLLLKKYLDDGLLFPTARANALFDYIHQDKKNNFI
                     QNYSKEQLSKTLNSSNNILGIEYIKNLIHLNSSIKPFTISRVQSEYNSEEIESDICSA
                     TAIRTSLKENTDLFHLKNVIPEKTYNILNEKIDNGFLPMFDDFFFDILKTIILRDMDT
                     LKEYFEVNEGIENRIYKDIFTSEFLHELQLAIKSKRYTLTKIKRTLNNILLGIKKSDV
                     QLIKNIDTIPYIRILAFNDKGREILKEIKNKSEIKIINKFSNISFSLDDTIFKTLISY
                     DIKATNIYNSIYYKNNKDLVKGPMDFYTHPIYVK"
     gene            complement(1565..2956)
                     /locus_tag="KPD58_001556"
     CDS             complement(1565..2956)
                     /locus_tag="KPD58_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBG0265333.1"
                     /translation="MYKLIDKKDIDFLIDTCGEENVLVGSDINEDFSHDELGGIEKYP
                     EVLVNVLETEQVSKIMKYAYKNNIPVTPRGQGTGLVGAAVAINGGIMINLCKMNKILE
                     VDYENLTLTVEPGVLLMTIGQYVQDRDLFYPPDPGEKSATIAGNINTNAGGMRAVKYG
                     VTRDYVRGLEVVLPNGEIINVGGKVVKNSSGYSIKDLLVGSEGTLGIVTKAILKLLPL
                     PKKSISLLIPFPDLSMAIETVPKIIKSKSIPTAIEFMERDVILAAEEFLGKKFPDNTS
                     DAYLLLTFDGNSTEDIEKEYEKVANLCLENGALDVFISDTQERNDSIWSARGAFLEAI
                     KASTTQMDECDVVVPRDKIAEFIRYTHELQDKLKIRIKSFGHAGDGNLHIYILKDGMD
                     DNTWKIRLKETFDYMYKKSRELSGQVSGEHGIGYAKKEYLHESNSDAYMMLIKNIKLA
                     FDPKNILNPGKIY"
     gene            complement(2967..4187)
                     /locus_tag="KPD58_001557"
     CDS             complement(2967..4187)
                     /locus_tag="KPD58_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HBG0265334.1"
                     /translation="MSKIVVNQDKITDLKRILEICPFGAIEEKSGIVEISAGCKMCKL
                     CVKSGPKGAFEFIESSKVQINKDEWRGIAVYVEHHNGNIHPVTYELIGKAREMASKIK
                     QPVYCVFVGKDIKDKCSNLISYGVDEVFVYDEDEFKDFRIEPYSKAIENFIDKIKPTI
                     VLVGGTTLGRSLAPRLAARFRTGLTADCTILDIQSNTDLDQIRPAFGGNIMAHINTPN
                     NRPQFATVRYKIFSAPEKIENTTGKITLCKVEKKELKSKIKVLSVKEKNKEVGLEEAE
                     VIVVASRAIKKQEDMEMIYKLADKLNAQVAGTRPVIEAGWIDAKKQIGLSGRTVKPKL
                     IITCGVSGAVQFVAGMQGADYIVAINKDDKAPILDVAHLALIGDIYDIIPKLIEKIEN
                     NKDNNQKYMASAAK"
     gene            complement(4201..4989)
                     /locus_tag="KPD58_001558"
     CDS             complement(4201..4989)
                     /locus_tag="KPD58_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBG0265335.1"
                     /translation="MEILTCIKQVPGTTSVEVDETTGVLKRDGVDSKMNPYDLYALET
                     ALRIKEDKKANLKVLSMGPPQAKKVIEESFMMGADEGALISDRRFGGADVLATSYTIS
                     QGIKKMGKVDLIICGKQTTDGDTAQVGPEVAEFLDIPHVTNVTKLIEVKDESIVVEID
                     MPNDLQVCEIEYPCLITVEKDIFQPRLPSFKLKLNTKDREIPVYSLDDFEDKDENNYG
                     LNGSPTQVVRIFPPKPNTDKNIVRGNADELSFALVNKLEELKLV"
     gene            complement(5006..6280)
                     /gene="larA"
                     /locus_tag="KPD58_001559"
     CDS             complement(5006..6280)
                     /gene="larA"
                     /locus_tag="KPD58_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HBG0265336.1"
                     /translation="MVKLKVPYSKQGMILEIPDERFLGVLESKSDNYIEHKTQEEIVE
                     ESMNNPIGSNSLEELVKGKKNVVIITSDHTRPVPSRITMPIILRRIRNVNPEIDVKII
                     VATGFHRPSTREELIYKMGEEIVDNEDIVMHISTDDKSMCKVGILPSGGDLYINKLAY
                     EAELLIAEGFIEPHFFAGFSGGRKSVLPGIASAKTIMYNHCSDFIDSDNSRTGKLNNN
                     PIHEDMVYAAKVAKLAFILNVVIDKDKKIIASFAGDVEKAHTKGCRFVTELSKVNSIK
                     SDIVVTTNGGYPLDQNIYQSVKGMTAAEATCKDGGVIIMISACNDGHGGQSFYENVAN
                     ADSPKQLLDKIRSVDRVDTIPDQWEFQILARILSKYTVIMVTDKCNPEMIKKMHMKHA
                     FNFKEALEMANSIVGKESKVVVIPDGVSVIVK"
     gene            complement(6398..7111)
                     /locus_tag="KPD58_001560"
     CDS             complement(6398..7111)
                     /locus_tag="KPD58_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBG0265337.1"
                     /translation="MFNSIASKKVYEQVIEQIQYKILNGELKKGDKLLSERELSEQMN
                     VSRTSIREAIRVLETMGVIESRQGEGNFICTNIEKTLIEPLSMIFKLNNGTLEDILEL
                     RIILEIEIAKLASKRITSSEVIELKHIIDEMRVETNKKDNNRVLVLLDQKFHSKLATL
                     SKNYLIQSLFMTASKLFDGFIEDAREKIIAEPFNENILLKQHEAIYNAVVENDVELAC
                     EKAKEHMDFISKNYRKNEN"
     gene            7236..8495
                     /locus_tag="KPD58_001561"
     CDS             7236..8495
                     /locus_tag="KPD58_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HBG0265338.1"
                     /translation="MKKNKIIAFFAPSIIVGVLVLGIILYPNEAIKSAREGFSIWSNV
                     LVPSLLPFIIGANLIVDLKIVDIIGFIINPITRFVFNVSGKSALAFAISTVSGYPVGV
                     SLASEFRSNGQISKYEAQRLVSFCSTSGPLFIIGSVGTGMFQNSNLGYLMILCHYLGT
                     ILVGLFFRNYGNENLPKTKLDLKTNIKNVINIRNSSGNGFFVLFGNAVFNGVNTLLTV
                     GGFVIVFSVVFKILSLFNVISLIASVIYLPLSFLGVSKELCQAFVSGLFEITIGCNKV
                     SSITSSPEILRASLASFLIGFSGLSILAQCCTFLSKTDIDLKLYILSKFAHGVLAAIF
                     IFILYPIANSAVLVSSFADTYNVIYNNLIWFYYLSYYDTILQIVVIIYLISAVFIAKK
                     HMKTLSTNGKLMRYKNTFFTKKFSRKK"
     gene            8775..9341
                     /locus_tag="KPD58_001562"
     CDS             8775..9341
                     /locus_tag="KPD58_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG0265339.1"
                     /translation="MATRPIEIDEYKKIMELLHTGFTYSENGVEKRFRKNPKVALALM
                     LEANLGLRISDILRLKIGNFKGNMLEINEKKTGKLQYRPVNKNIIESINEHARKYNLK
                     SNDYLVNIKTKAIQKQLRIICKYLNLYNISTHSFRKLYATTQFEKSNNNLELVKELLN
                     HSSVATTQRYIRVTQQAINEASENFFIG"
     gene            complement(9542..10747)
                     /locus_tag="KPD58_001563"
     CDS             complement(9542..10747)
                     /locus_tag="KPD58_001563"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HBG0265340.1"
                     /translation="MKIAILGYGTVGSGLIDLIENNTSKRDIEVVGILVKNKEKHKYK
                     KYFDKITTDIEDIFDKDIDILVEVIGGLNPAFDYVTRALNKKIHVVTANKDLLAEKGS
                     DLIELANLNNVSIKFEASVAGGIPVLKPLIESLEGNNIKSINAILNGTCNFILSKMYD
                     ENLPYDVALKQAQELGFAEVNPDADVLGYDSARKLSILSTLSYGKIVYWKDLLLEGIT
                     SIDEKDIEYAKKLNCKIKLVARSKYESGEVSGFVRPALVDNNNMLSKIDNEFNVVILV
                     GDSVGELSFVGKGAGRGATGSAVYADIIDIIDNRSSNIKSFSKGKLDLSGLIEDECSA
                     VVRFGGYNNKDGILECLDKYVDSYDIIDDEELAIFVSAKSEHEIDRVLEIVKRNNYSE
                     SAKKLLKID"
     regulatory      complement(10850..10956)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(11048..11416)
                     /locus_tag="KPD58_001564"
     CDS             complement(11048..11416)
                     /locus_tag="KPD58_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HBG0265341.1"
                     /translation="MLKGKQRAYLRSLANTLKPTTQIGKEGVTESFLEQLDGMLRTRE
                     LVKVTILENAGLDTKETANAVCEALRAEFVQAIGFKFTIYKRNIEEPKILFPGHEQAK
                     AKTKNNNVTKKGKPTKRAVR"
     gene            complement(11468..12745)
                     /gene="obgE"
                     /locus_tag="KPD58_001565"
     CDS             complement(11468..12745)
                     /gene="obgE"
                     /locus_tag="KPD58_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HBG0265342.1"
                     /translation="MFIDKARIFVKAGNGGNGSVAFRREKYVPAGGPDGGDGGRGASI
                     IFEVDLGLRTLMDFKYQKKYQAQNGGDGSKGKRAGKNGENLVLKVPAGTVIRDEATGL
                     VLADLKKEGDTAIVAKGGIGGKGNQHFANAVRQAPAFAKSGTDGEERWITLELKMIAD
                     VGLLGFPNVGKSTFLSVVTKAKPKIANYHFTTLTPNLGVVQTKFGDSFVLADIPGIIE
                     GASEGIGLGHEFLRHVERTKVLIHIVDISGLEGRDPIEDFDKINDELKLYNEKLSKRP
                     QVVVANKFDILEDESKFEKFKSELEGRGYTVFKMSAATRQGIDEVIAYVSKMLKEVED
                     VELVSEEEMYRPELDIGTEEELSIDIEDGVYVVTGKALRRIMYSVNFDDMESLQYFQK
                     AMESQGVFDRLREMGIEDGDVVKIYELEFEFYN"
     gene            complement(12856..13146)
                     /gene="rpmA"
                     /locus_tag="KPD58_001566"
     CDS             complement(12856..13146)
                     /gene="rpmA"
                     /locus_tag="KPD58_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBG0265343.1"
                     /translation="MLNMNLQLLASKKGVGSSKNGRDSISKRLGVKRFDGQLVTAGSI
                     IVRQRGTKIHPGTNVGKGSDDTLFALVDGTVKFERKDKKRKKVSIYPVAIAE"
     gene            complement(13160..13477)
                     /locus_tag="KPD58_001567"
     CDS             complement(13160..13477)
                     /locus_tag="KPD58_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HBG0265344.1"
                     /translation="MIKVKYYYNDDFLLQGFCLKGHADFAEIGYDIVCAAVTSNAIAV
                     INSLDKLQKIEFEKVVGEEGHIECIVKDTHVKDAQLLLNHFQLAVKEIKREYPKNIKI
                     LKK"
     gene            complement(13492..13803)
                     /gene="rplU"
                     /locus_tag="KPD58_001568"
     CDS             complement(13492..13803)
                     /gene="rplU"
                     /locus_tag="KPD58_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBG0265345.1"
                     /translation="MYAIVKTGGKQYKVSEGDVLFVEKLEANAGDVVTLNEVLACSKD
                     GELKLGSPVVEGASVQAKVVEQGKAKKVIVFKYKAKKDYRRKQGHRQSYTKIVVEKIN
                     A"
     gene            complement(13904..15325)
                     /locus_tag="KPD58_001569"
     CDS             complement(13904..15325)
                     /locus_tag="KPD58_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HBG0265346.1"
                     /translation="MKKIVIESLIGSQKTAVLEDERLTELFVEDNLNKKTVSNIYRGI
                     VKKVIPGIEACFVDIGFKKLAYLQLKKGSTIKSGQDILVQINKEEIGTKGAKLNTEIS
                     ISGRYIVYIPSNDRTTISNKITDEKERFRLKKITKAVNKENLGLIIRTEAQGCTHDEI
                     KKDIEELKLKYENILKEYKLGIGPKLLYKSLDFATKYVKDNVNDDIESIITNSYDKYS
                     ELKSILRGIDKTYVDKLCLEENRDVFDLYRIESKIEKLLNKKVWLKSGGYLIIEKTEA
                     LTVIDVNTGKFIGTGKLDETVYKTNLEAAKEIVRQLRIRDIAGIIIIDFIDMHKKKHQ
                     KEILHILEEEFNKDKRKAEVLGMTKLGLVEVARRREKESIDKYYLMSCPCCDGEQTIK
                     SVHYILDSIEKEIMRISEHTVYKNIMVEFNDFIFEQIKEYYMDIIDKIGEKYNIKISL
                     NANSTLKHNKTNVIFDKIVDNKM"
     gene            complement(15343..16044)
                     /locus_tag="KPD58_001570"
     CDS             complement(15343..16044)
                     /locus_tag="KPD58_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2344 domain-containing protein"
                     /protein_id="HBG0265347.1"
                     /translation="MSKIIRVKFKKEGDMIYISHLDLQRLLQRAFRRAEINLSHSQGF
                     NPHPKMSYGNALALGTESQGEYVDIEIEEDDLSVEEFLNKVSIQLPDGIDFIKAKEID
                     RQTPSLSSVIDYGEYLFNIDLKRPMTKEFVKRKVIDFMNNKEIIITKKNKKGKMVEVD
                     IRPMIRTFDVLNLEDEHITLTATIATGSKTNLNTNILIPKILEMFELDIDPLDVDILR
                     RDLYVLEDGELVTPM"
     gene            complement(16046..17890)
                     /locus_tag="KPD58_001571"
     CDS             complement(16046..17890)
                     /locus_tag="KPD58_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HBG0265348.1"
                     /translation="MNKVDLKKILKKVEKPARYLGNEINSIHKDTSNSELIRYAHCFP
                     DLYEVGMSHLGSHILYDVINKDEDVFCERVYSPAVDMENIMREKSIPLFALESREPIT
                     NFDFVTFTLQYELSYTNILNILDLANIPILKEERTLEDPFIMVGGPCAYNSEPLADFV
                     DVVILGEGEEVNLEVVNEYKEWKKNKTTREDFLYKISSIEGVYIPSFYDVKYNEDGTV
                     QSVTPNREGITKNPTKRIIKDVETVDYPEKLIVPYIDTVHDRIVLELFRGCTRGCRFC
                     QAGMIYRPIREKSVKRLKEILDKLVKNTGYDEISLSSLSTSDYSKLSELTDYLVDEYA
                     SNNIGISLPSLRLDNFSMEIADKIQQVRKSGLTFAPEAGTQRLRDVINKGVTEEDLEN
                     ATERAFEMGWNSVKLYFMIGLPTETYEDLDGIAKLAYKVIDIYRKVNGGKLKRSFSVT
                     VSTSTFVPKPFTPFQWHGQDTTEEVINKQRHLVNKLRNNNIKYNYHDSKTSLMEAVVA
                     RGDRRIGKVIYDAFRLGAKFDGWAEHFNLDIWKEAMEKNNLSIDFYAHRNRNYEEVFP
                     WDHIDVGISKKFLIREDENAKKEKITSDCRHNCNGCGINIHDIGRGLC"
BASE COUNT         5708 a         3286 c         1923 g         7053 t
ORIGIN      
        1 taaagataaa aggagtattt tatgaacata cttggattaa tagttgaata taacccattt
       61 cataatggac acatatatca tttatctaaa tctatagaaa aaacaaatgc aacacatact
      121 gtagcaataa tgagtggaaa ttttctacaa agaggcgaac cagctttatt tgataaatac
      181 actagagcaa aaattgctgt cgaaaatggt gtagacttag ttatagaatt accaacttta
      241 tttgcttgtc aaagtgctga aattttttct catggtgcta taacaatttt aaattcacta
      301 aactgcataa attctatatg ttttggtagt gaagaaggaa atacagaaat tctttataat
      361 ctagctgaaa ttttagcaaa tgaaccaaat gaatttaagt tacttttaaa aaaatattta
      421 gatgatggtc tgttatttcc aacagctaga gctaatgcac tttttgatta tatacatcaa
      481 gacaaaaaaa ataattttat acaaaattat tcaaaggaac aattatcaaa aacattaaat
      541 tcatcaaaca acatactggg tatagaatac ataaaaaacc tcatccattt aaacagtagt
      601 ataaaaccat ttactatctc cagggtccaa tcagaatata attccgaaga aattgagagt
      661 gatatttgtt ctgctactgc tataagaact tcacttaaag aaaatacaga cctttttcat
      721 ttaaaaaatg ttattccaga aaaaacttat aacatcctaa atgaaaaaat agataatggt
      781 tttttaccta tgtttgatga cttcttcttt gatattttaa aaacaattat acttagagat
      841 atggacactt taaaagagta ttttgaagta aatgaaggca tagaaaacag aatctataaa
      901 gatatcttta catctgaatt tctacatgaa ctacaattag caattaaatc caagcgatac
      961 actttaacca aaatcaaacg aactctaaac aatatattgt taggtattaa aaaaagcgac
     1021 gtgcaactta taaaaaacat agatactata ccttatataa gaatacttgc ttttaatgat
     1081 aaaggtagag aaattctaaa agagataaaa aataaatctg aaataaaaat aataaacaag
     1141 ttttcaaata tatcattttc attagatgat acgattttta aaacactcat aagttatgat
     1201 ataaaagcta caaatatata taattcaata tattataaaa acaataaaga tttagtaaaa
     1261 ggccctatgg atttttatac tcatccaata tatgtaaagt agttttttaa aataactata
     1321 tttataaaaa tcattttatt aactctttat tatataagat acaatagtct tactttattt
     1381 atgtacacat gcttttagga tgacaacatc aacttaaata ttatgttact ttctaaattt
     1441 aagtaaatat tgccatccta aaaattttac aatttagctt cagatttaaa agtaaagttt
     1501 aaaatattat atttaaatct aatttaatat tttttgtttt ataaatatta ttaaattaat
     1561 atttctaata tattttccct ggatttaaaa tatttttagg gtcaaaagct aactttatat
     1621 ttttaattaa catcatataa gcatctgaat ttgattcatg taagtactct tttttagcat
     1681 atcctatgcc atgttcacca gaaacttgac cactaagttc tctagatttt ttatacatat
     1741 agtcaaaagt ttcttttaat cttattttcc atgtattatc atccatacca tcttttaaaa
     1801 tatatatgtg tagatttcca tctcctgcat gaccaaaact ttttattctt atttttaatt
     1861 tgtcttgaag ctcgtgagtg tatcttataa actctgctat cttatctctt ggaacaacga
     1921 catcacattc atccatttgt gtcgtagatg cctttatagc ttctaaaaat gcacctctgg
     1981 ctgaccatat agaatcgttt ctttcttgtg tatctgatat aaatacatct aatgcaccat
     2041 tttctaaaca taaattagct actttttcat attctttttc tatatcttca gtgctatttc
     2101 catcaaatgt aagcaaaaga taagcatctg atgtattatc tggaaacttt ttacctaaaa
     2161 attcttctgc tgccaatatc acatctcttt ccataaattc tatagccgtt ggtattgatt
     2221 ttgattttat tattttagga acagtttcta ttgccattga taaatctgga aatggtataa
     2281 gcaaacttat acttttcttt ggtaaaggaa gcaattttaa tatagcttta gtaactattc
     2341 ctaaagtacc ttctgagccc actaataaat cctttatact atatccagaa ctatttttaa
     2401 ccacctttcc acctacattt ataatttctc catttggtag tacaacttca agtcctctta
     2461 cataatccct tgtaacgcca tattttactg ctctcatacc acctgcatta gtgtttatat
     2521 tacctgcaat tgtagcactt ttttcacctg ggtcaggtgg gtaaaataaa tctctatctt
     2581 gaacatattg acctattgtc atcaataaaa cacctggttc tactgtcaat gttaaatttt
     2641 cataatcaac ttcaagtatt ttattcattt tacatagatt aatcattatt ccaccattta
     2701 tggcaacagc agcacctaca agaccagtac cttgacctct tggtgtgact ggtatattat
     2761 ttttatatgc gtatttcatt attttagata cttgttcagt ttcaagtaca tttactaaaa
     2821 cttcagggta cttctcaatc cctccaagtt catcatgtga aaaatcttca tttatatcac
     2881 ttccaactaa tacattttct tctccacaag tatctattaa aaaatctata tcttttttat
     2941 cgattaattt atacatttct atccccctat ttagctgctg atgccatata tttttgatta
     3001 ttatctttgt tattttctat tttttcaatc aatttaggaa taatatcata aatatcacct
     3061 attaaagcta aatgtgctac atcaagtata ggcgctttat catctttatt tattgctact
     3121 atatagtcag caccctgcat ccctgcaaca aattgaactg ctccagatac accacaagtt
     3181 attatcaatt taggttttac agttctacca ctaagaccta tttgtttttt agcatctatc
     3241 caacctgctt ctattacagg tcttgttcct gccacttgag cgtttaactt atcagctaat
     3301 ttatatatca tctccatatc ttcttgtttt tttattgctc tacttgccac aactatcact
     3361 tcagcttctt ccagaccaac ttctttattt ttttctttta cactcagtac cttaatttta
     3421 gattttagtt cttttttttc tactttacat aaagttatct ttcctgtagt attctctatt
     3481 ttttctggtg ctgaaaatat tttatatctt acagtggcaa attgtggtct attattaggt
     3541 gtgtttatat gagccattat atttccacca aatgctggtc ttatttggtc taaatcagta
     3601 tttgattgaa tatcaagtat tgtacaatct gctgtaagac ctgttctaaa tctagcggca
     3661 agtcttggag ctaatgacct acctaatgta gttccaccaa ctaatactat cgttggtttt
     3721 attttatcta taaagttttc tattgctttt gaatatggtt ctatcctaaa atctttaaat
     3781 tcatcttcat catatacaaa tacttcatcc accccataag aaatcaaatt actgcactta
     3841 tcttttatgt cttttcctac aaatacacaa tacacaggtt gttttatttt tgatgccatc
     3901 tcacgagctt tacctataag ctcgtacgtt acaggatgta tatttccatt atgatgttca
     3961 acatatactg caattcccct ccattcatct ttattaattt gtactttact actctctata
     4021 aattcaaatg cgccttttgg tccacttttg acacacaact tgcacatctt acagccagca
     4081 cttatttcta ctatacctga cttttcttct atagccccaa aaggacatat ttctaaaatt
     4141 ctctttaagt cagttatttt atcttgattt acaactatct ttgacattta ttactccccc
     4201 ttacaccaac tttaattcct ctaatttatt tacaagtgca aaacttagtt catctgcatt
     4261 tcctcttaca atatttttat ctgtatttgg ctttggagga aatattctta caacttgagt
     4321 aggagaacca ttaagtccat agttattttc atctttatct tcaaagtcat ctaagctata
     4381 tacaggtatt tctctatctt tagtattaag ttttaattta aatgaaggta gtcttggttg
     4441 aaaaatgtct ttttctacag ttattaaaca tggatattca atctcacata cttgtaaatc
     4501 atttggcata tctatttcaa caactatact ttcatcttta acttcaatta attttgtaac
     4561 gttagtaaca tgaggtatat ctaaaaattc agctacttca ggtcccacct gtgctgtatc
     4621 accatcagta gtttgttttc cacaaattat taggtctacc tttcccatct tcttaatacc
     4681 ttgtgatatg gtataggaag tagccaatac atctgctcct ccaaatctcc tatcacttat
     4741 taatgctcct tcatcagctc ccatcataaa actttcttca attaccttct ttgcctgagg
     4801 aggacccatg ctaagtactt ttagattcgc tttcttatct tcctttattc taagagctgt
     4861 ttctaaagca tataggtcat agggattcat ttttgaatct actccatctc tttttagtac
     4921 tcctgtagtt tcatcaactt ctactgaagt cgttccagga acttgtttaa tacatgtcaa
     4981 aatttccata aagtacactc cttttttatt taactataac tgatactcca tcaggtataa
     5041 ctactacttt gctctcctta ccaactatac tattcgccat ttctaaagct tctttaaagt
     5101 taaatgcatg tttcatatgc atttttttta tcatttcagg attacactta tctgttacca
     5161 ttatcacagt atatttagat aatattctcg ctagtatctg aaattcccat tggtctggaa
     5221 tcgtatctac tctatcgaca cttctaattt tatctaaaag ttgttttgga gaatctgcat
     5281 ttgctacatt ttcataaaaa gattgacctc catgaccatc attacatgct gaaatcatta
     5341 ttattactcc accgtcttta caagtagctt cagctgctgt cattccttta acagattgat
     5401 aaatattttg gtctagtgga tatccaccat ttgtagttac aacaatatca gattttatag
     5461 aatttacttt tgataactct gttacaaatc tacatccttt agtatgtgct ttttcgacat
     5521 caccagcaaa ggaagcaatt atttttttat ctttatctat tacaacattc aatatgaatg
     5581 ctaattttgc aactttagct gcatatacca tatcctcatg aataggatta ttattcaatt
     5641 ttccagttct tgaattatca ctatctataa agtcactaca atgattgtac attatagtct
     5701 ttgcagatgc gattcctgga agtacacttt ttctgccacc tgaaaaacct gcaaagaaat
     5761 gaggttctat aaaaccttct gctatcaaaa gttctgcctc atatgctagt ttgtttatat
     5821 ataaatctcc acctgaaggc aatatcccta ctttacacat agatttatca tcagtagata
     5881 tatgcataac tatatcttca ttatctacta tttcttctcc cattttataa attagctctt
     5941 cccttgtact tggcctatga aatccagttg ccactataat cttcacatca atttctggat
     6001 taacattcct aatccttctt aatataattg gcatagttat tctacttggt acaggtcttg
     6061 tatggtcact tgtaattatg actacattct ttttcccctt aaccagttct tccagagaat
     6121 tagagcctat aggattattc atagattctt caactatctc ttcttgagtc ttatgttcta
     6181 tataattatc cgattttgac tccaaaacac cgagaaaacg ttcatcaggt atttctaaaa
     6241 tcattccttg ttttgaatac ggtactttta actttaccat ttttaatctc ccattataat
     6301 aaactataaa attggtatgt ggtcatacca gttttacatt tatatgatag taccattatc
     6361 taccatttgc aagaataatt tattttataa caaactttta attttcattt tttctataat
     6421 ttttacttat aaaatccata tgttcctttg ctttctcaca tgcaagttct acatcatttt
     6481 ctacaactgc attgtatata gcttcatgct gtttaagcaa tatattttca ttaaaaggtt
     6541 ctgcgattat tttttctctt gcatcttcta taaatccatc aaataatttt gatgctgtca
     6601 taaaaagact ttgaattaaa taatttttac ttaatgttgc tagtttactg tgaaattttt
     6661 ggtctaataa caccagtact cgattattat cttttttatt agtttctacc ctcatttcat
     6721 ctattatatg tttcaattct ataacttcag aagaagttat tctctttgaa gcaagttttg
     6781 caatctcaat ttctaggatt attctaagct ctaatatatc ttcaagtgta ccattattaa
     6841 gtttaaaaat catagataaa ggttctatta aagttttttc tatatttgta catataaaat
     6901 ttccttcacc ctgtctgctt tctatcactc ccatagtctc taatacccta atagcttccc
     6961 ttatggaagt tctactcaca ttcatttgtt cagacagttc tctttctgat agtaatttat
     7021 cccccttttt taactctcca tttagtattt tatattgtat ttgctcaatt acttgttcat
     7081 aaactttttt actagctata ctattaaaca ttttttcctc caatatgata atcgttgttg
     7141 taatatatat ctattatata tcttaagcca ttacattcat atatttttat tacttttaat
     7201 ataatatctt ataactcaaa tttacaggtg atattatgaa aaaaaataaa attattgctt
     7261 tttttgctcc atctatcata gttggagttc tagttttagg gattatccta tacccaaatg
     7321 aagctataaa atctgcaaga gaaggctttt ctatatggtc taatgtttta gttccatcac
     7381 tactaccatt tataattgga gcaaacttaa ttgttgattt aaaaatagtg gatattatag
     7441 ggtttatcat caatcccata actagatttg tatttaatgt ttcaggaaag agcgctttag
     7501 cttttgctat atctacagta tctggttatc cagtaggagt aagtttagct tctgaattta
     7561 ggagtaatgg acaaatttct aagtatgaag cacaaagact tgtgtctttt tgttctacat
     7621 caggaccttt attcataata ggttcggttg gtacaggtat gttccaaaat tcaaacttag
     7681 gttatttaat gattctctgc cattatctag gaactatatt ggttggtctt ttctttagaa
     7741 attatggtaa tgaaaatctt ccaaagacaa aattggattt aaaaacaaat ataaaaaatg
     7801 taatcaacat tagaaattca agtgggaatg gtttttttgt tttatttgga aatgctgtat
     7861 ttaatggagt aaatacatta cttacagttg gtggatttgt aattgttttt tcagtagtat
     7921 ttaaaatact atctcttttt aatgtaatct cattaatagc atcagttata tatttgcctc
     7981 tttcattttt aggtgttagt aaagaactct gtcaagcatt tgtaagtggt ctatttgaaa
     8041 ttactatagg atgcaataaa gtatctagta taacaagttc tccagaaatt ttaagagcat
     8101 ctttagctag ttttttgata ggatttagtg gattatccat cctagctcaa tgttgtacat
     8161 ttctatcaaa aacggacata gatttaaaat tatacatact aagtaaattt gcgcatggag
     8221 ttttagctgc tatctttata tttatacttt atccaattgc aaactctgct gtgttggttt
     8281 ctagctttgc tgatacatac aacgttatat ataataattt aatctggttt tattatctta
     8341 gctattatga tacaattctt caaattgtag taataattta tctaatctca gccgtattta
     8401 ttgcaaaaaa acatatgaaa actttatcta caaatgggaa actaatgcga tataaaaata
     8461 cttttttcac taagaaattt tcacgaaaaa aataaaacta aaaataaaac taataaaact
     8521 taactaaata cttccatttt tttattatat ttgtcataat tttaataacc tttttaatta
     8581 gtttaacagc atagttattt catcacaatt taataatttt attagaaata tttttcaaaa
     8641 aattctcctt aatcacatat atcacacttg tataagttta ctgggaaatt tttccattat
     8701 ataatatttc atttatatta tataataaat accgacctaa acttaccaaa tcaaactaag
     8761 aggagtggtt gaaaatggca acaagaccta tagaaataga cgaatataaa aagataatgg
     8821 aactattaca tactggcttt acttatagtg aaaatggcgt tgaaaaaaga tttagaaaaa
     8881 atcctaaagt tgcattggca ctaatgttag aagctaattt aggtctaaga atatcagata
     8941 ttttaagatt aaagataggt aattttaaag gtaatatgtt ggaaataaat gaaaaaaaga
     9001 cgggcaaatt acagtataga ccagttaata aaaatataat tgagtccata aatgaacatg
     9061 caagaaagta taatcttaaa tcaaatgact acttagtaaa tataaagaca aaggctattc
     9121 aaaaacagtt gaggataatt tgtaaatact taaatctgta taatataagt acacatagct
     9181 ttagaaagct ctatgctact actcaatttg aaaagagtaa caataattta gagttggtta
     9241 aagaactttt aaatcacagc tctgtagcca ctacacagcg atatataagg gtaactcagc
     9301 aagctattaa tgaagctagt gaaaatttct ttattggtta attataacgt atacattttt
     9361 ctattatctt attaattcac cagatattaa aaaagagtgt ctcaaaatga actttttagt
     9421 tcatgagaca ctcttttttt ataaaatcaa atatattttt attatttcaa cactggaaaa
     9481 aaggcttatc tctctatttt gagacaccct attaaatttt cataattctt gcataaacat
     9541 attaatctat cttcaataat tttttagctg attcactgta attatttctt tttacaattt
     9601 ccaaaactct atcaatctca tgttctgact ttgcacttac aaaaatcgcc aattcttcat
     9661 catctataat atcataacta tctacatatt tatctaaaca ttctaaaatt ccatctttat
     9721 tattataccc accaaatcta actactgcac tacactcatc ttctataagt ccacttaaat
     9781 ccaatttccc ttttgaaaaa gactttatat ttgaacttct attatctatt atatctataa
     9841 tatctgcata aacagcactt cctgttgcac ctcttccagc tcccttacct acaaaagaaa
     9901 gttcacctac tgaatcacct actaaaataa ctacgttgaa ctcattatct atctttgaaa
     9961 gcatattatt attgtccact aatgctggtc taacaaatcc acttacttct cctgattcat
    10021 acttacttct agcaaccaat tttatcttac aatttaattt ttttgcatat tctatatctt
    10081 tttcatctat tgaagttatt ccttctaata ataaatcttt ccaatatact atttttccat
    10141 aagataaagt agacaatata gataactttc tagcactatc atatcctaaa acatctgcat
    10201 ctggattaac ttctgcaaat cccaactctt gtgcctgttt taaagcgaca tcatacggta
    10261 aattttcatc atacatcttt gataatataa aattacaagt cccatttaaa atagcgttta
    10321 tgctctttat attgttgcct tccagtgatt ctatcaatgg ttttaaaact ggaataccac
    10381 ctgccacaga tgcttcaaac ttgatgctaa cattatttaa gttagctaat tctataaggt
    10441 cactaccttt ttcagccagt aaatctttat tagctgtcac tacatgtatt tttttattta
    10501 acgctcttgt tacataatca aaagctggat ttaatcctcc aattacttca actagtatat
    10561 caatatcctt gtcaaatata tcttctatat cagtagttat cttatcaaag tacttcttat
    10621 acttgtgttt ttctttattt ttaactaaaa ttccaacaac ttctatatct ctttttgaag
    10681 tattattttc tattaaatca attaatccac ttcctactgt tccataccct aatattgcta
    10741 tcttcactgt aacatacccc catcattttg attatattaa attatttata tatataaata
    10801 taccaataaa aaaagcttga gtataaactc aagccatatg acgggtttat actcatcttt
    10861 cagcataaat gctgcaggat ttagcaccaa tgtataatta aatatactgg ttgccgggct
    10921 tcacagggcc agtccctctg ccactcttga taagattgct aatttaaatt tatgtttaat
    10981 agtataatac aatttcttcc aattgtcact atttttttaa cttatttccc taaaaggata
    11041 taaactatta tctaacagct cttttagttg gtttaccctt ttttgtaaca ttattatttt
    11101 tagtctttgc tttcgcctgt tcatgtcctg gaaaaagtat tttaggctct tcaatattcc
    11161 tcttgtaaat agtaaattta aaaccaatag cctgcacaaa ttcagctctt aaagcttcac
    11221 atactgcatt agcagtttct ttagtatcaa gacctgcatt ttctaatata gtaactttta
    11281 ctaattctct agttcttaac ataccatcta attgctctaa gaaactctct gtaacacctt
    11341 ctttaccaat ttgtgtagta ggctttaaag tatttgccaa agatcttaaa tacgcccttt
    11401 gttttccttt taacatattc tcacctctat aattctatac ttaaattatc tataatctat
    11461 atttaaatta attgtaaaac tcaaattcta attcataaat tttaaccaca tctccatctt
    11521 ctattcccat ttctctaagc ctatcaaaaa caccttgact ctccatagct ttttggaaat
    11581 attgaaggga ttccatatca tcaaaattta cagaatacat tattcttcta agtgctttac
    11641 ctgtaactac atacacacca tcttcaatat ctatacttaa ctcttcttca gtaccaatat
    11701 caagttctgg tctatacatt tcttcttctg aaactagctc cacatcttct acttctttta
    11761 gcattttaga aacataggct attacttcat ctatgccttg acgagttgct gctgacattt
    11821 taaatactgt atatcctctg ccttctaatt cactcttaaa cttctcaaac ttactctcat
    11881 cttctaatat atcaaattta tttgcaacta caacttgtgg tctctttgaa agcttttcat
    11941 tgtacaattt aagctcatca tttatcttgt caaaatcttc aatagggtct cttccctcta
    12001 atccagatat atctacaata tgtatcaata cctttgttct ctcaacatgt cttaagaact
    12061 catgacctaa tcctatgcct tcagaagcac cctctataat tccaggtata tctgcaagta
    12121 caaaactatc tccaaactta gtttgaacaa cacctagatt tggtgtaagt gttgtaaaat
    12181 gatagtttgc aattttgggt ttagctttag ttacaactga tagaaatgtt gatttaccta
    12241 catttggaaa tcctaaaaga ccaacatctg ctatcatttt aagttcaaga gttatccatc
    12301 tttcttctcc atctgtacca gattttgcaa aagctggagc ttgtcttact gcatttgcaa
    12361 agtgttggtt acctttacca cctataccac cttttgcaac tatagctgta tctccttctt
    12421 tttttaaatc tgctaataca agacctgtag cctcatctct tattactgta cctgctggta
    12481 cttttaatac taaattttct ccatttttac cagctctttt tcctttgctt ccgtcgccac
    12541 cattttgagc ttgatatttt ttctggtact taaagtccat tagagttcta agccccaaat
    12601 caacttcaaa tatgatgctt gctccacgac caccatctcc accgtctgga ccaccagctg
    12661 gaacgtattt ttctcttcta aatgctaccg aaccatttcc accatttcca gccttaacaa
    12721 atattcttgc cttatctata aacaaattct tcacctactt aattaaaaac ttttaattag
    12781 cattaccaaa aataccatta taaattcatc agtattataa gaaaaaggag tgcctcaaca
    12841 ctccttttat gtatattact cagctatagc tactggatat atactaactt tttttctctt
    12901 cttgtctttt ctttcgaatt taacagtacc atcaactaat gcaaataaag tatcatcact
    12961 accttttcca acattagttc ctggatgtat tttagttcct ctttgtctaa ctattatact
    13021 tccagcagtt actaattgac catcgaatct cttaacacct aatcttttag atatagaatc
    13081 tctaccattt ttagaagaac ctaccccttt tttagatgct aataattgta aattcatgtt
    13141 taacatatgg agtcaccccc tactttttta aaatttttat attttttggg tattcccgtt
    13201 taatttcttt aaccgctaat tgaaagtgat ttagtaataa ttgtgcgtct tttacatgtg
    13261 tatctttgac tatacactct atatgacctt cttctcctac tactttttca aattcaatct
    13321 tttgtaattt gtctaaagaa tttataactg caatagcatt agatgtaact gcagcacata
    13381 ctatatcata gcctatttca gcaaaatcag catgtccctt taaacaaaaa ccttgcaaca
    13441 agaaatcatc attataataa tatttaactt ttatcataac ttaaccaata actaagcgtt
    13501 tattttttct acaactatct tagtgtatga ttgacgatgt ccttgttttc ttctgtaatc
    13561 tttttttgct ttatacttga aaactataac tttcttagct ttaccttgtt caacaacttt
    13621 agcttgaaca gatgctcctt ctactactgg agaacctaat tttaattctc catctttaga
    13681 gcaagctaat acttcgttta aagtaacaac atcacctgcg ttagcttcta atttttcaac
    13741 gaataataca tcaccttcag aaactttata ttgctttcca ccagtcttta ctatagcgta
    13801 cattaaatac acctcctcgg cctcaaactc gccagtacaa ggtacttcat ttgaagattt
    13861 aaacctttac tgtgcggcat tacaattatt aattataaaa tatttacatt ttattgtcaa
    13921 ctattttatc gaaaataaca ttggttttat tgtgctttaa tgtactattt gcgtttaaag
    13981 aaatctttat attatatttt tcaccaattt tatcaattat atccatataa tattctttta
    14041 tttgttcaaa aataaaatca ttaaattcta ccattatgtt tttgtaaaca gtatgttcac
    14101 ttattctcat aatttctttt tcaatactat ctagtatata atgtacagat tttattgttt
    14161 gttcaccatc acaacatgga caagacatta aataatactt atcaatggat tctttttctc
    14221 tacgcctagc aacttctact aaaccaagtt ttgtcatacc taatacttca gcttttcttt
    14281 tatctttatt aaattcttct tctaggatgt gtaaaatctc cttttgatgc ttctttttat
    14341 gcatatcaat aaaatcgatt attattatcc cagctatgtc tctaattcta agctgtctta
    14401 caatttcttt tgcagcttca agattagttt tataaacggt ttcatcaagt ttaccagttc
    14461 caataaactt acccgtattt acatctataa ccgtaagagc ttctgttttt tcaattatta
    14521 aataacctcc actctttaac catacttttt tatttaataa tttttctatt ttagattcta
    14581 ttctgtataa atcaaataca tctctatttt cctcaagaca cagtttatca acataagtct
    14641 tatctatacc tcttaaaata gacttaagtt cagagtactt atcataacta ttggtaatta
    14701 tagattcaat atcatcattc acattatctt ttacatattt agttgcaaaa tctaaagact
    14761 tatataataa tttcggacct ataccaagtt tatattcttt taaaatgttt tcatacttta
    14821 atttcaactc ttctatatct ttctttattt catcatgagt gcaaccttga gcctcagtcc
    14881 ttattattaa tcctagattt tccttattta ctgccttagt aatttttttt aatctaaatc
    14941 gttctttttc atcagtaatt ttatttgata ttgtagttct atcattggaa ggtatataga
    15001 caatatacct tccagaaata ctaatctcag tgtttaattt tgcgccttta gttcctattt
    15061 cttctttatt tatctgaacc aaaatatcct gaccagattt tatagtactt ccctttttta
    15121 attgtaaata agcaagtttc ttaaatccaa tatctacaaa acaagcttct attcctggta
    15181 taactttttt tacgattcca cgatatatat tggatacagt cttcttattt aaattgtctt
    15241 caacaaaaag ttctgttaat ctttcatctt caagtactgc tgttttctgg gaccctatta
    15301 gggactcaat aactatcttt ttcacaactt cacctctcga gcttacatag gagtcactaa
    15361 ttccccatct tctaatacat ataaatctct tctcaatata tcaacatcta atgggtcaat
    15421 atcaagttca aacatctcta atatctttgg tataagtata ttagtattta aattagtttt
    15481 tgagcctgta gctatagtag cagtaagagt aatatgttca tcttctaaat ttaaaacatc
    15541 aaaggttctt atcataggtc ttatatctac ttcaaccatc ttaccttttt tattcttttt
    15601 tgttatgatt atttctttat tattcataaa gtcaattact tttcttttta caaactcttt
    15661 cgtcataggt cttttcaaat ctatattaaa caaatattct ccatagtcta tcacagaaga
    15721 aagagaaggt gtttgtctat caatttcttt tgctttgata aaatctatcc catctggaag
    15781 ttgaattgat actttattta aaaattcctc tactgataaa tcatcttctt ctatttcaat
    15841 atctacatac tcaccttgac tttctgtccc taaagccaaa gcattgccat aactcatttt
    15901 agggtgagga ttaaatcctt gagaatgtga taagtttatt tcagctcttc taaacgctct
    15961 ttgtaacaat ctctgaaggt caagatgtga tatatatatc atgtcgcctt cctttttaaa
    16021 ttttactctt attatcttac tcatattagc aaagccccct tcctatatca tgtatattta
    16081 ttccacaacc attgcagtta tgtctacaat cagaagtgat tttttctttt ttagcatttt
    16141 cgtcttctct aattaagaat tttttactaa tacctacatc tatatggtcc caaggaaata
    16201 cctcttcata gtttctattt ctatgtgcat aaaaatctat agaaagatta ttcttttcca
    16261 tagcttcttt ccatatatct aaattaaaat gttctgccca tccatcaaat ttagcaccta
    16321 gtctaaatgc atcatatata acttttccta ttcttctatc gcctctagct actactgctt
    16381 ccatcaaact agtctttgaa tcatgataat tgtattttat attgttattt cttaatttat
    16441 ttactaaatg tctctgctta ttaataactt cttctgtagt atcttgacca tgccattgga
    16501 aaggtgtaaa tggctttggt acaaatgtag aagtactcac tgtaacacta aaacttctct
    16561 ttagctttcc tccatttact tttctataaa tatcaataac tttataagct aatttagcta
    16621 taccatctaa atcttcataa gtctcagttg gtaaaccaat cataaaatac aacttaacac
    16681 tattccaccc catctcaaaa gctctttctg ttgcattttc caaatcttct tctgtaacac
    16741 ctttatttat aacatctcta agtctttgag ttcctgcttc tggagcaaaa gtaagtccag
    16801 attttcttac ttgttgtatt ttatcagcaa tttccattga aaaattatct aatctaaggg
    16861 atggaagcga aatcccaata ttattagatg catattcatc taccaaataa tcagtcaact
    16921 ctgataactt actataatca ctcgtactaa gtgatgataa tgatatttca tcatatccag
    16981 tattcttaac taatttatct aatatttctt taagtctttt aacactcttt tctcttattg
    17041 gtctgtatat catacctgct tgacagaatc tacatcctct agtacatcct ctaaataatt
    17101 ctagtactat tctatcatga acagtatcta tataaggtac tattagtttt tctggataat
    17161 ctacagtctc aacatctttt attatccttt tagtaggatt tttagtaatc ccttctctat
    17221 taggagttac actttgaact gtaccatctt cattgtactt gacatcataa aaacttggta
    17281 tgtatactcc ttctatgcta gatattttat ataaaaaatc ttctctagtc gttttatttt
    17341 tcttccattc tttatactca tttactactt ctaaattaac ctcttcacct tcacctaata
    17401 ttactacatc tacaaaatct gctaatggtt cggaattata agcacaagga ccaccaacca
    17461 ttataaatgg gtcttccaaa gttctttctt cttttaatat tggtatattg gctaaatcta
    17521 atatatttaa gatattagtg tatgaaagtt catattgaag tgtaaaagtg acaaaatcaa
    17581 agtttgttat aggctctctt gactctaatg caaataatgg tatacttttt tctctcatta
    17641 tattttccat atcaactgca ggagaataca ctctctcaca aaatacatct tcatccttat
    17701 ttattacatc atataatata tgactcccta agtgactcat accaacttca tataaatctg
    17761 gaaagcaatg tgcatatctt atcaattcac tattactagt atctttatga attgaattta
    17821 tttcatttcc aagatatcta gcaggctttt ctactttttt taagattttt ttcaagtcta
    17881 ctttattcat ttattaaaat tcctccaaac aagctcttta tttttattct tatttataag
    17941 taatattata cacgataaaa tattttctat
//
LOCUS       DAELLV010000056        17695 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.056, whole genome shotgun sequence.
ACCESSION   DAELLV010000056 DAELLV010000000
VERSION     DAELLV010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17695
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.056"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            170..1774
                     /gene="rho"
                     /locus_tag="KPD58_001572"
     CDS             170..1774
                     /gene="rho"
                     /locus_tag="KPD58_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBG0265349.1"
                     /translation="MRRIFLILDDKVLEGLGSKTLVELREIAKELKIKSITTYKKNEL
                     IEIINSKSKNEEKVNEETKEKNEDIKEDIKNRKENIERESIEKEVEYKSPTKSYNKKE
                     IDTQNISQNQEHRNQRNDYNKTTSVNDSNKSSNISNRMVRNNNKNYYMPKQVDESKIV
                     DEFNTSKEDEVVGVLEILPDGFGFLRGSNYLSTEGDVYVSPSQIRRFNMKTGDKIKGI
                     TRHPKSGEKFRALLYVQKINDENPDTAIQRNAFETLTPIFPEERLTLETNRNEIATRI
                     IDLISPIGKGQRGLIVAPPKAGKTVLLKSVANSIAKNHPNVELIVLLIDERPEEVTDM
                     KESIEGDVIYSTFDQVSSHHVKVAEMVLNRAQRLVEHGKDVVILLDSITRLARAYNLT
                     ISPTGRTLSGGIDPGALHGPKKFFGAARNIRQGGSLTILGTALVETGSRMDDVIFEEF
                     KGTGNMELHLDRKLAEKRIFPAIDIYKSGTRRDDLLLDDEEKTALWRLRREMSNNSVM
                     EITDKVIELIKRTKDNKEFVKSIKNL"
     gene            1927..2127
                     /gene="rpmE"
                     /locus_tag="KPD58_001573"
     CDS             1927..2127
                     /gene="rpmE"
                     /locus_tag="KPD58_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HBG0265350.1"
                     /translation="MQKEIQPKYNPVEVRCACGNTFVAGSTKDEIKVEICSECHPFYT
                     GKQKNIEKGGRIDKFKKRFKMD"
     gene            2272..3165
                     /locus_tag="KPD58_001574"
     CDS             2272..3165
                     /locus_tag="KPD58_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HBG0265351.1"
                     /translation="MAKQAVGGQAVIEGVMMQSKDKRAVAVRKSDGEIALKEDRIKSW
                     VRDKNIDKIPFVRGSFVMIDTMIQGIKSLNFSSEFFMEEAEEDKFDLFIKKIFKDKAN
                     DIIIIFSLVIAMLLSAGLFIFIPTLVGGAFSKVMPNDFMLNLIEGIIRIAILFAYIVL
                     ISRSKDIERVFQYHGAEHKSIYCYENDLELTVENARKFKRLHPRCGTNFLFIVMAVSI
                     ILFAFFGWPNPILRIFMRIICVPIVAGLSYEVIRVLGKYDNGFTKIIAYPGMMLQYFT
                     TKEPDDEQLEVALEALKAVVD"
     gene            3183..4031
                     /gene="prmC"
                     /locus_tag="KPD58_001575"
     CDS             3183..4031
                     /gene="prmC"
                     /locus_tag="KPD58_001575"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBG0265352.1"
                     /translation="MTIKDIIIKYSDKLKDISDTPRLDTELLLQKTLGVDRLYIHLNL
                     NKELTEEQKTKFMGFAEERLNGRPIAYIVENREFMGLDFFVKEGVLIPRPDTETLVEE
                     IIEICREKKDVSILDIGTGSGAITISLAKYIENSKIMSFDISETALEIAKKNAIINEV
                     GEKIKYINSDLFTAISDSNIKFDIIVSNPPYIKKQDIETLHKQVKDYEPYNALEGGED
                     GLDFYRRITEQGKKYLNKCGILAYEVGHNQAEDVINIMKSNGYKKIYTKKDIQGIDRV
                     VIGYNI"
     gene            4075..5139
                     /gene="prfA"
                     /locus_tag="KPD58_001576"
     CDS             4075..5139
                     /gene="prfA"
                     /locus_tag="KPD58_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBG0265353.1"
                     /translation="MLKKLEVLEDTYKDLSEKIGDPDVINDQKVWQKYIKEHADLEPI
                     VMKYREYKSVLDSIKESKEILQEESDEELRELAKMELAEMEEKVAPLEEEIKILLLPK
                     DPNDDKNVIVEIRGGAGGDEAALFAGDLFRMYSRYAERRRWKIELLSASDTGVGGYKE
                     VSFMIKGKGAYSRLKYESGVHRVQRIPSTESGGRIHTSTSTVAVLPEVEDVEVEINPN
                     DLRIDVFRSSGNGGQSVNTTDSAVRVTHIPTGEVVSCQDGKSQLKNKEQALKILKARL
                     YDKALAEQHKDIAAERKSQVGTGDRSERIRTYNFPQGRISDHRINLTLYKLDAFLDGD
                     IDEMIDALITVDQTEKMTAI"
     gene            5268..5948
                     /locus_tag="KPD58_001577"
     CDS             5268..5948
                     /locus_tag="KPD58_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HBG0265354.1"
                     /translation="MILKVTIIGLLAGVIGTGLGGVISAIFKREVDKYLSFFMGLSGG
                     IMLAVVVFDLMKESMDKMGIINTVIFTFVGALITMYIKTKLDVSGNMASGYLIFISIL
                     LHNLPEGLAIGSSFMSTESLGITLAIVIGLHNIPEGLAMALGLVCNKMKLSKVILFTV
                     IAGLPMGLGSFLGVYFGGVFTSLIGVFLATAGGTMMYVVLEEIFPHSKSVYCIIGFLL
                     GTMIVNYI"
     gene            5977..7017
                     /locus_tag="KPD58_001578"
     CDS             5977..7017
                     /locus_tag="KPD58_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBG0265355.1"
                     /translation="MKTIISNIDINNIDYEEIKIQAKLLREGKTVIFPTETVYGLGAN
                     ALDENAVKKIYEAKGRPSDNPLIVHIYEKEEVYDLAKDISDKAKLVIEKLWPGPITII
                     LNKKDIIPYKTSGGLDTVAIRMPSNVIARAIIKEAGIPIAAPSANISGRPSPTKAKHV
                     YEEMNGRVDGIVLGGDSNFGLESTVLDLTEETPMILRPGSITKEVLEELLGEVKLDPS
                     LSKKEDNQKAKAPGMKYKHYSPNADVYIISGKRENVATKINDMIKSNRDKGLKTGVMC
                     ISRNREFYDGEVIDLGNSLEEVASNLFDALIEMDKKGVDVIYSEEFPKTGVGQAIMNR
                     LLKSAGYKVIKA"
     gene            7034..7483
                     /locus_tag="KPD58_001579"
     CDS             7034..7483
                     /locus_tag="KPD58_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HBG0265356.1"
                     /translation="MNILIVCTGNTCRSPMAEAILRKAIKESGRSIEEYSISSAGIST
                     ANGMGASENSIEVLKEIGIDLSNHRSKVITKKLIDESDIILTMTKSHKEILVQAVPKC
                     KEKVYTFKGFANKNEEDISDPFGGNLDIYRSTMREIMYSVNEIVKKI"
     gene            7500..7952
                     /gene="rpiB"
                     /locus_tag="KPD58_001580"
     CDS             7500..7952
                     /gene="rpiB"
                     /locus_tag="KPD58_001580"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HBG0265357.1"
                     /translation="MKIGLGCDHGGYNLKKEIISYLESKGIECVDYGTNNATDSVDYP
                     VYGEIVANSVINKEVDYGILCCGTGIGISLAANKVPGIRCAVVSDVFSAKMSKAHNDA
                     NMLSLGERVLGKGLALEIVEAWINTDFEGDRHARRVNMIKSIEEKHNK"
     gene            7988..8617
                     /gene="upp"
                     /locus_tag="KPD58_001581"
     CDS             7988..8617
                     /gene="upp"
                     /locus_tag="KPD58_001581"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBG0265358.1"
                     /translation="MSKVVETNHPLIQHKLTLMRDKNTGSKDFRELLTEIAMLMGYEI
                     TKDIPLKDVEIETPIQKTSSKVVAGKKLAIIPILRAGLGMVDGLVSLMPAAKVGHVGL
                     YRDPETLKPVEYYCKLPQDIGERDIIVVDPMLATGGSAVAAIDLLKSKGAKSIKLANL
                     VAAPEGIAEVQKYHDDVDIYVASVDERLNEHGYIIPGLGDAGDRLFGTK"
     gene            8918..9733
                     /locus_tag="KPD58_001582"
     CDS             8918..9733
                     /locus_tag="KPD58_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HBG0265359.1"
                     /translation="MKNCIVVRGAGDLATAVIHKLHNSGFKVVALEIEEPLAIRRQIS
                     FCEAVYEKKVTVEGVECIFCNTEKEIEEVLSQHKVALIIDPKGEKLSKLNPIVLVDGI
                     LAKKNLGTTKDMAPLTIALGPGFCAGEDVDVVIETMRGHNLGRIITNGRAEKNTGVPG
                     AIKGFSKERVMHSQYDGIMENVCKIGDIVEKGDLLCYINNYEKKHEVRATISGVLRGL
                     LKDGAKVNKNLKILDIDPRKEEVKNAFTISDKARCIAGGVLEAILNNGIYPQN"
     gene            9959..10396
                     /locus_tag="KPD58_001583"
     CDS             9959..10396
                     /locus_tag="KPD58_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HBG0265360.1"
                     /translation="MRPSWDEYFMEIAEVVKKRSTCIRRQVGAVIVRDKQILTTGYNG
                     SPRNLEHCENIGCKRQELNIPSGERHELCRALHAEQNAIIQAAHNGISVDGATLYVTT
                     RPCVLCAKMCINAGILKIVYEGDYPDDMSTELLKEAGIELIKF"
     gene            10581..10808
                     /locus_tag="KPD58_001584"
     CDS             10581..10808
                     /locus_tag="KPD58_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HBG0265361.1"
                     /translation="MSKRNTHTEIAKLFSLISQIGLMIVISILGCTFLGKFLDSKLNT
                     TPVLTIIFLLLGVGGAFSGVYKTLIVYTRRK"
     gene            10813..11199
                     /locus_tag="KPD58_001585"
     CDS             10813..11199
                     /locus_tag="KPD58_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HBG0265362.1"
                     /translation="MDIKLLNQVRSVTKGIVIFDVVLIVLMLITSTLSKPVLLGLIFG
                     SIIAMLNFRLLAISLEKSVTFPAGKAQAYASAQYAVRLFIMAVVLIASATVPHLNIIG
                     TALGLLSTKFVILSKNFINKLKRKEA"
     gene            11202..11906
                     /gene="atpB"
                     /locus_tag="KPD58_001586"
     CDS             11202..11906
                     /gene="atpB"
                     /locus_tag="KPD58_001586"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBG0265363.1"
                     /translation="MNQAHYYLIGNFKISETILVSWGIMAFLAIASYFLTRNLKKVPT
                     SKVQIFLEFAVGGLAKMVTDVMGKNTVKRMPYMIPYIGSLFLFFACSNLSPLLGLRSP
                     TTDLDTTAAWAMITFFMIYFAGVKFKGLSYFKELAQPLAIMLPINIIGELARPISLSF
                     RPFGNILGGTIIMGLFYQLLGFISSLIPGLSIPIGEILIPVPLHLYFDLFAGLLQAYI
                     FIMLTMVFISSAGELD"
     gene            11975..12235
                     /gene="atpE"
                     /locus_tag="KPD58_001587"
     CDS             11975..12235
                     /gene="atpE"
                     /locus_tag="KPD58_001587"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HBG0265364.1"
                     /translation="METAIVAAASAIGAGIAVATGIGAGIGQGIAAAKAAEAVGNQPE
                     AKGDITSTLLLGVAIAESSAIYGLVISIILLFVNPFFKYLGM"
     gene            12358..12855
                     /gene="atpF"
                     /locus_tag="KPD58_001588"
     CDS             12358..12855
                     /gene="atpF"
                     /locus_tag="KPD58_001588"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBG0265365.1"
                     /translation="MEKALVGITWEFVFQIVNTFIIFLLLRKLLFKPVLNIIESREND
                     IKSDLAEGEKAKNEGLALKKEYESKINFAKDEGQEIIKQATIRAEQKSDDIVNTAKKD
                     ALDIKEKANKDIEQERQKVINEIKNDISNIALLAASKVIEKDLDKSKHEELIENFIKE
                     VGEAK"
     gene            12852..13397
                     /locus_tag="KPD58_001589"
     CDS             12852..13397
                     /locus_tag="KPD58_001589"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBG0265366.1"
                     /translation="MINVIANRYAEALFQLGEEENSTDVLFKELEKVVDMMTKVSKDF
                     YKVLKSPLVSKSEKKNLVEIIFSKEVSSNIKNFLKVLVDKDRISYLEDIELAYKELLN
                     KKNNVIDGVAISAIPMSETDIKELEVKLSNKYNKNVTIENVVDKTILGGVLVRIGNEQ
                     IDGTVKTRLDKMKEKLSEVIS"
     gene            13414..14916
                     /locus_tag="KPD58_001590"
     CDS             13414..14916
                     /locus_tag="KPD58_001590"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003405060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBG0265367.1"
                     /translation="MNLKPEEISSIIKQQIKNYENKVELTDTGSVLTVGDGIASVYGL
                     EKAMSGELLEFPGEIYGMALNLEEEVVGAVILGDDSEIKEGDIVKRTGRIVEVPVGEA
                     LIGRVVNSLGQPIDGKGPIAYTKTRPVESEAPGIIDRRSVYEPLQTGIKSIDSMIPIG
                     RGQRELIIGDRQTGKTSIVIDTILNQKGKDVICIYVAIGQKRSTIAQLVSSLEKGGAL
                     DYTIVVSATASESAPLQYIAPYAGAAMGEEFMYNGKHVLIVYDDLSKQAVAYREMSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDELGGGSMTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLQPELFYSGVRPAVDPGISVSRVGGSAQIKAMKKVAGTLKLAYSQYREL
                     AAFSQFGSDLDEDTKKRLAQGERIVEILKQGEHQPIKVENQVMIIYAVINNHLEDIPI
                     DNIARFESELYAFVDNNYPEISRKILGGEDFTHDLTDAINEFKEKFVVEV"
     gene            14944..15822
                     /gene="atpG"
                     /locus_tag="KPD58_001591"
     CDS             14944..15822
                     /gene="atpG"
                     /locus_tag="KPD58_001591"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HBG0265368.1"
                     /translation="MAGAGIKAIKTRMGTVESTKQITKAMELVASSKLRKAKEKAEDS
                     REYFNTLYNTVKDIAQTTRGIRNEFLKEREVKNKCYVVVAGDRGLAGGYNSNVLKESV
                     SHMKGKKETVITVGKKAHEFFSKRNYNIAKDINSVEACGYEDATEISQTIIDLYKKGE
                     VDEVYIAYTKFKSALSQEVEILKLLPLVFEKDTDEQGMAVKEEKQNRSKVQYIPSAEA
                     VLSYIVPQFISGTVYGGILESFASEQGARRTAMESATDNANEMLSNLELSYNRARQSA
                     VTQEITEIVGGVEALK"
     gene            15835..17229
                     /gene="atpD"
                     /locus_tag="KPD58_001592"
     CDS             15835..17229
                     /gene="atpD"
                     /locus_tag="KPD58_001592"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBG0265369.1"
                     /translation="MANVGKVVQIVGAVLDVKFDGEQSLPNLLNALVIKLGDKEIVAE
                     VAQHIGDDTVRCIAMSATDGLVRGMEVVDTGGPISVPVGDETLGRIFNVLGKPVDGKP
                     APKSAPKLPIHRPAPAYDELETTAEILETGIKVVDLLAPYLKGGKIGLFGGAGVGKTV
                     LIQELINNIAKQHGGISVFSGVGERTREGNDLYGEMSESGVINKTALVFGQMNEPPGA
                     RMRVALTGLTMAEHFRDEQGQDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLA
                     TEMGALQERITSTKKGSITSVQAVYVPADDLTDPAPATTFSHLDAKTVLSRQISSLGI
                     YPAVDPLESTSRILDPSIVGKEHYEVARGVQSILQRYKELQDIIAILGMDELSDEDKL
                     IVARARKIQRFLSQSFTVAEQFTGNPGQYVPVKETVRGFKEILEGKHDDLPESAFLFV
                     GTIEDAVRKAKGSM"
     gene            17232..17492
                     /gene="atpC"
                     /locus_tag="KPD58_001593"
     CDS             17232..17492
                     /gene="atpC"
                     /locus_tag="KPD58_001593"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HBG0265370.1"
                     /translation="MASEFSLKVVTPSNTFYDGDVEMIVVRTTKGDKGVLKGHIPFVS
                     GIPVGTLKIKKEGSFKEAKIAEGFINVTQDKTVILTESAEWI"
BASE COUNT         7028 a         1981 c         3460 g         5226 t
ORIGIN      
        1 taagattgga cttgaaatac agtaatatag taggtaataa tgttttaaat tttatttatt
       61 gaccataata tataaataat gatattatta gacttaactg tttaaataaa acttttcttt
      121 ttcatgcctt caatttccaa aataagtatg tatataaatg aatatataga tgaggaggat
      181 ttttttgatt ttagatgata aagtattaga aggattaggt agtaagacct tagttgagct
      241 acgagagata gctaaagaat taaaaataaa atcaattact acatataaga aaaatgagtt
      301 aattgagatt ataaattcca aaagtaaaaa tgaggaaaaa gtaaacgaag agactaaaga
      361 aaaaaatgaa gatattaaag aagacataaa gaatagaaaa gaaaatatag agcgtgaaag
      421 tatagaaaaa gaagttgaat ataagagccc taccaaatca tacaacaaga aagaaataga
      481 tacccaaaat atttcccaaa accaagaaca tagaaatcaa agaaatgact ataataagac
      541 aacgagtgta aatgatagca ataagtcatc aaatatatct aatagaatgg tcagaaataa
      601 taataaaaac tattatatgc ctaaacaagt tgatgaatct aagatagtag atgaatttaa
      661 cacatcaaaa gaggatgaag tagtaggtgt acttgaaatt ctgccagatg gatttggttt
      721 tttaagaggt tcaaattatt tgtctactga aggagatgtt tacgtttcac catctcaaat
      781 aagaagattt aatatgaaaa caggagacaa gattaaggga ataactagac atccaaaaag
      841 tggagagaaa tttagagcac ttttatatgt tcaaaaaata aatgatgaaa accctgacac
      901 agctatacaa agaaatgcgt ttgagacatt aacaccaata tttccagaag aacgacttac
      961 actggaaact aatagaaatg aaatagctac aagaattata gatttaattt cacctatagg
     1021 aaaaggacaa agaggattga tagtagcacc tccaaaagct gggaaaacag ttcttttaaa
     1081 aagcgttgca aatagcatag ctaaaaatca tccaaatgtt gaacttatag tactccttat
     1141 agatgaaaga ccagaggaag ttactgatat gaaagaatct attgaaggag acgttatata
     1201 ttccactttt gaccaagtat caagtcatca tgtcaaggtt gcagagatgg tgttaaatag
     1261 agctcaaaga ttagttgaac atggtaaaga tgtagtaatt ttactagata gtataactag
     1321 attggcaagg gcatataacc ttacaatatc tcctacaggt agaactttat ctggaggtat
     1381 tgaccctggt gcacttcatg gacctaaaaa gttctttggt gcagcaagga atattagaca
     1441 gggtggttct ttgacaatat taggaacagc attggttgaa actggttcta ggatggatga
     1501 tgtaatattt gaggaattta aaggtacagg aaatatggag ctacatttag atagaaaatt
     1561 agcagaaaaa agaatattcc ctgcaataga tatttacaag tctggtacaa gaagagatga
     1621 tttattgctt gatgatgaag aaaagactgc tctttggaga ttaagaagag aaatgagtaa
     1681 taattcagta atggaaatta cagataaagt cattgaactt ataaagagaa ctaaggacaa
     1741 taaagagttt gtaaaatcaa taaagaattt gtaaaatcaa taatacaaat gcaaatatgg
     1801 attatataaa ctaatagaaa aatcacttga atggcataat aagttgtgct ataataatgt
     1861 agttgataat gtaaaaagaa agttaatata attaattttt ttaaataaag agaagaggtg
     1921 actataatgc aaaaggaaat acaaccaaaa tacaatccag ttgaagtgcg ttgtgcttgt
     1981 gggaacacat ttgttgctgg ttcaactaag gacgaaataa aagttgaaat atgttcagag
     2041 tgccatccat tctatacagg aaaacaaaag aacatagaaa aaggtggaag aatcgacaaa
     2101 ttcaagaaaa gattcaaaat ggactaattt ttataaaagt ggagttggag gaagccaact
     2161 cctattttat tacaaaaaaa cattcatatt ctactttaaa ttgtcttttt gtattataat
     2221 aataaatatg taaaattgag tattctaaat acaataaaat gaggtgagtg gatggcaaaa
     2281 caagctgtag gtgggcaagc tgttattgaa ggtgtaatga tgcagtcaaa agacaaaaga
     2341 gcggtagctg ttagaaaaag tgacggagaa atagcactaa aagaagatag aataaaaagt
     2401 tgggtaagag ataagaatat agataagata ccttttgtta gaggttcttt tgttatgata
     2461 gatacaatga ttcaaggcat aaaaagttta aacttttctt cagagttttt tatggaagaa
     2521 gctgaagagg ataaatttga tttatttata aagaaaattt ttaaagataa ggcaaatgat
     2581 ataattataa tattttcatt ggtcatagca atgttacttt ctgctggatt attcattttt
     2641 atacctacat tggtaggagg agctttttct aaagtaatgc ctaatgattt tatgcttaat
     2701 ttaatagaag gaatcataag gatagctata ttatttgcat atatagtatt gatttcacga
     2761 agtaaggata tagaaagagt ctttcaatat catggagcag aacataaatc tatatattgc
     2821 tatgaaaatg atttagaact tacagtagag aatgctagaa aatttaaaag attacatcct
     2881 agatgtggta caaatttcct atttatagtt atggctgtat caataatatt gtttgcattt
     2941 tttggttggc caaatccaat tcttagaata tttatgagaa ttatatgtgt gcctatagta
     3001 gcaggtttat cttatgaggt aattagagtt cttgggaaat atgataatgg atttactaaa
     3061 attattgcat atccaggaat gatgttgcaa tattttacaa caaaagagcc tgatgatgag
     3121 caattagagg ttgctttgga agcattgaaa gcagtagtag attagagaaa aggatatttg
     3181 ttatgacgat aaaagatata attattaaat attcagataa actaaaagat ataagtgata
     3241 ctccaagatt agatacagag ttattgttac aaaaaacatt gggtgttgat agattatata
     3301 ttcacttaaa tctgaataaa gagctaactg aagaacaaaa aacaaaattt atgggttttg
     3361 cagaagaaag attaaatgga agaccaatag catatatagt tgaaaatagg gagtttatgg
     3421 gattagattt ttttgttaag gaaggcgtat tgattccaag accagataca gaaactttag
     3481 tagaagaaat aattgaaata tgtagagaaa aaaaagatgt aagtatatta gatataggaa
     3541 ctggttctgg agctattaca ataagtttag caaagtacat tgaaaattct aaaatcatgt
     3601 cttttgatat atcagagaca gcattagaaa tagctaaaaa aaatgctatt ataaatgaag
     3661 ttggtgaaaa aataaagtat attaattctg atttatttac agcaataagt gatagtaaca
     3721 taaaattcga tatcatagtt tcaaatccac catatattaa aaaacaagat atagaaactc
     3781 tgcacaaaca agttaaagat tatgaaccat ataatgctct tgaaggtgga gaagatggat
     3841 tagactttta tagaagaata acagaacaag gtaagaaata tttaaataag tgtggaatat
     3901 tagcatatga agttggtcat aatcaagctg aagatgtaat aaatattatg aaaagtaatg
     3961 gatataaaaa aatatacaca aagaaggata ttcaaggcat tgatagagtt gttataggtt
     4021 acaatatatg atataatcaa caattgatat gttacattta atcgaggtga aagtatgtta
     4081 aaaaaattag aggtattgga agatacatat aaagacttaa gtgaaaaaat aggtgatccg
     4141 gatgtaataa atgatcaaaa agtttggcag aaatatataa aagaacatgc tgacttggaa
     4201 cctattgtta tgaaatacag agagtataaa agcgttttag atagtataaa agaatcaaaa
     4261 gaaattttac aagaagagtc agatgaagaa ttaagagaat tagctaaaat ggagttagct
     4321 gagatggaag aaaaagtagc acctctggag gaagaaataa aaatactatt attgcctaaa
     4381 gaccctaatg atgataagaa cgttatagtt gaaattagag gtggagctgg aggagatgaa
     4441 gcagctctat ttgctggtga tttatttaga atgtactcaa ggtatgctga aagaagaaga
     4501 tggaaaatag aattattgag tgcaagtgat actggagttg gtggatataa agaagtatct
     4561 tttatgataa aaggtaaagg tgcttattca agattaaagt atgaatctgg agttcataga
     4621 gttcaaagaa taccatcaac tgaatctggt ggaagaatac acacatcaac ttctactgtt
     4681 gcagtattac cagaagttga agatgtagag gttgaaataa atccaaatga tttaagaata
     4741 gatgttttcc gttcttcggg taatggagga caaagtgtca atactactga ctctgctgtt
     4801 agggtaacac atatacctac aggtgaagta gtatcttgtc aagatggaaa atcacaatta
     4861 aaaaataaag agcaagcttt aaaaatatta aaagctagac tttacgataa agcattggca
     4921 gaacaacaca aagatatagc agcagaaaga aaaagccaag tcggaacagg agacaggtca
     4981 gaaagaataa gaacatataa tttccctcaa ggtagaataa gtgaccatag aataaattta
     5041 actttatata agttagatgc attcttagat ggagatatag atgaaatgat agatgcttta
     5101 attactgttg accaaacaga aaaaatgact gcaatataag taatgtttta aagaagctgt
     5161 ttcttcctag aaattatttt ctattttgaa acagctattt tgcattttag gtcatgttat
     5221 catattctat tgtatataat tgttatatac aagtatagga gggttatatg atactaaaag
     5281 taacaataat tgggcttttg gcaggtgtta tagggacagg attaggagga gtaatatcag
     5341 ccatttttaa aagagaggtc gataaatatc taagtttttt tatgggatta tctggtggaa
     5401 taatgttggc tgtagtggtg tttgatttaa tgaaagaatc catggacaaa atggggataa
     5461 tcaatacagt tatatttacg tttgttggag ctctcataac tatgtacata aaaacaaaat
     5521 tagatgtgtc tggaaatatg gcatctggat atcttatatt cataagtata ttactacata
     5581 atttaccaga aggactagcc attggttctt cttttatgtc aactgaaagt ttaggtatta
     5641 cacttgctat agttataggt ctacataata taccagaagg gttagctatg gcattaggct
     5701 tagtttgcaa taaaatgaaa ctaagtaaag ttattttgtt tactgtaata gctggattac
     5761 ctatgggtct aggaagtttt ttaggtgttt attttggagg agtatttact tctcttatag
     5821 gagtatttct tgctacagca ggaggtacta tgatgtatgt tgtattggaa gaaatttttc
     5881 cacattcaaa aagtgtgtat tgtataattg gtttcttgtt aggtacaatg attgtaaatt
     5941 atatataaaa ttaaaatgaa agcaggcata caaaagatga aaactattat aagtaatatt
     6001 gatataaata atattgatta tgaggaaata aaaatacaag caaaacttct tagagaaggt
     6061 aaaacagtta tttttccaac tgagacagta tatgggttgg gtgcaaatgc attagatgaa
     6121 aatgcagtaa agaagattta tgaagctaaa ggaagaccta gtgacaatcc attgatagtc
     6181 catatatatg aaaaagaaga agtatatgac ttagccaaag atataagtga taaagctaaa
     6241 cttgttatag aaaagctttg gccaggacct ataacaatta tattaaataa aaaagatata
     6301 attccataca aaactagtgg agggcttgat acagttgcaa taaggatgcc ttctaatgtt
     6361 attgccaggg caataataaa agaagctggt atacctattg cagctccttc tgcaaatatc
     6421 tcaggtcgcc catctccaac aaaagcaaaa catgtatatg aagagatgaa tggaagagtg
     6481 gatggaatag ttttaggagg agacagtaat tttggattgg agtcaactgt attggattta
     6541 acagaagaga ctcctatgat actgagacca ggaagtataa ctaaagaagt tttagaggaa
     6601 cttcttggag aagtaaagct tgacccatca ttaagtaaaa aagaagataa tcaaaaagct
     6661 aaagctcctg gaatgaaata taaacattat tctccaaatg cagatgtata tataatatct
     6721 ggaaaaagag aaaatgtagc aacaaagatt aatgatatga taaaatctaa tagagacaaa
     6781 ggtctaaaga caggtgtaat gtgtattagt agaaaccgag agttttatga tggagaagta
     6841 atcgatttag gaaactctct tgaagaagtt gcaagtaatc tgtttgatgc tcttatagaa
     6901 atggacaaaa agggtgttga tgtcatatat tcagaagagt tccctaagac tggagttgga
     6961 caagcaatta tgaatagact actgaagtca gcaggataca aagtgataaa ggcttaagag
     7021 ggagttgtaa aatatgaata tacttattgt gtgtacaggt aatacttgta gaagtcctat
     7081 ggctgaggct atactaagga aagcaattaa agaatctgga agaagtattg aagaatattc
     7141 tatatcatca gcaggtatat ctacagcaaa tggaatgggt gcttctgaaa attctataga
     7201 agtactaaaa gaaatcggta tagacttatc aaatcataga agtaaagtaa ttactaaaaa
     7261 attaattgat gaatcagata taatactaac aatgactaaa tctcataaag aaattttagt
     7321 acaagcagtt ccaaaatgca aagaaaaagt gtatactttt aaagggtttg caaataaaaa
     7381 tgaggaagat atatctgacc catttggtgg aaatttggat atttatagaa gcactatgag
     7441 agaaataatg tattctgtca acgaaatagt aaaaaaaata tgaaacggag gataaacaaa
     7501 tgaagatagg attaggctgt gaccatggag gatataattt aaaaaaagaa ataataagct
     7561 atttagaaag taaaggtata gaatgtgtag attacggaac taataatgct acagattctg
     7621 ttgattatcc tgtttatgga gaaatagttg caaattcagt tataaataaa gaagtagatt
     7681 atggaatatt atgttgtggt acagggatag gaatatcttt agctgctaat aaggttcctg
     7741 ggataagatg tgcagttgta tcagatgtat tttctgcaaa aatgtcaaaa gcacataatg
     7801 atgcaaatat gttatcatta ggcgaaagag ttttaggaaa aggtctagca cttgagatag
     7861 ttgaagcatg gattaatacg gattttgaag gtgatagaca tgctagaaga gtaaacatga
     7921 ttaaatcaat agaagaaaaa cataataaat aataaaaatt acagttttat caggaggaat
     7981 tatcaaaatg agcaaagtgg tagagacaaa ccatccatta atacaacata aattaacttt
     8041 aatgagagat aaaaatactg gttctaaaga ctttagagag cttttaactg aaattgcaat
     8101 gttaatgggg tatgaaataa caaaagatat accattaaaa gatgtagaaa tagaaactcc
     8161 tattcaaaaa acatcatcta aagtagttgc aggtaaaaaa ttagcaataa ttccaatttt
     8221 aagagcaggt cttggaatgg tagatggatt agtgagctta atgccagcag caaaagttgg
     8281 acatgtagga ctatatagag acccagaaac attaaaacca gttgaatatt attgtaagct
     8341 tcctcaagac ataggagaaa gagatataat agtagttgac cctatgcttg caacaggggg
     8401 ttctgctgta gcagctatag atttacttaa gtctaaagga gctaaaagta taaaattagc
     8461 taatttagta gctgcaccag aaggtatagc agaagtgcaa aaatatcatg atgatgttga
     8521 tatatatgtt gcatcagttg acgaaagatt aaatgaacat ggatatataa ttcctggtct
     8581 tggtgatgct ggagatagat tgtttggaac taaataaaaa taaataacta taaatattta
     8641 ttagggactt tcttatatag tgtatataat tttatgtaag aaggttcttt tttatatatt
     8701 ttttctcaaa ataggaatta ttgaattgat aaatttctcg aattgagaat aaaaagtttt
     8761 taccaattaa aaaagattgc ttattttaca aggttttata gaatagtagt ctattttaat
     8821 gtggcatgtt agttgcttac gattaagatt ataggtatta agtgtattag ttaaattgag
     8881 aattgaatat taataatatt attgggagtg gtaatacatg aagaattgta tagtagtaag
     8941 aggggctgga gatttagcaa cagcagttat tcataaatta cacaacagtg gttttaaagt
     9001 agttgcgtta gaaatagaag agccattagc tataagaagg caaatttctt tttgtgaggc
     9061 tgtttatgaa aaaaaagtaa ctgttgaagg tgtagaatgt atattttgca atactgaaaa
     9121 agaaatagaa gaagttttat ctcaacataa agtagccttg attatagacc caaaaggaga
     9181 aaaattatct aaattaaatc caatcgtttt ggttgatgga atactcgcta agaaaaactt
     9241 aggtacaact aaagatatgg caccacttac aatagcttta ggtcctggat tttgtgcagg
     9301 agaagatgta gatgttgtta tagagacaat gagaggtcat aatctaggaa gaataataac
     9361 taatggtaga gcagaaaaaa atacaggagt accaggagca ataaaaggtt tttctaaaga
     9421 gagagttatg cattcacagt atgatggcat aatggaaaat gtatgtaaga taggtgatat
     9481 agttgaaaag ggagacctac tttgctatat taataattat gaaaaaaaac atgaagttag
     9541 agcaacaata tctggagtcc ttagaggttt attaaaggat ggagcaaagg taaacaaaaa
     9601 tttaaaaata ctagacatag accctagaaa agaagaagtt aagaatgctt ttacaatatc
     9661 agataaagct agatgcatag ctggaggggt tttagaagcc atactaaaca atgggattta
     9721 tccccaaaat taatttatat tagataaagc tattaatatt ttattaagat gatataataa
     9781 atttaataga ttaaagagag catatgacgg gtatttaaaa tttaaatggt aaaaatgctc
     9841 tttttttatt ggacattcaa ttaacatgtg tatataatat aaaaataagt atttatttag
     9901 gttgaaatct aataaatata taataccagt acataagcaa agaggagaaa agtaaattat
     9961 gagaccatca tgggatgaat acttcatgga aattgcagaa gtagtgaaaa agcgttcaac
    10021 ttgtattaga agacaagtag gagctgtaat tgtaagagac aaacaaatat taacaactgg
    10081 atataatggt tctccgagaa atcttgaaca ctgtgaaaat attggatgta agaggcagga
    10141 attaaatatt ccctcaggag aaaggcatga attatgcaga gcattacatg ctgaacaaaa
    10201 tgcgataata caagctgctc ataatggtat tagtgtagat ggggcaactt tgtatgttac
    10261 tacaagacct tgtgttttgt gtgcaaaaat gtgcataaat gcaggtatat tgaaaattgt
    10321 ttatgaaggt gattatccag atgatatgtc tacagaatta ttaaaagaag caggaataga
    10381 actcataaaa ttttagtgga taaaatttat aagacaataa atgtcaaatt atgacactga
    10441 aatacaatgg tatcaaatga ttaaaagaaa gtatttgaaa aatcatcaga ttagtagtaa
    10501 gatatatttt ataattaaat aaatttatga gatagattaa ttacatgtta tttattttat
    10561 aataatgagg gatactattt atgagtaaaa gaaatacaca tacagaaatt gcaaagttgt
    10621 ttagtttaat aagtcaaata ggattgatga ttgtaatatc aatattagga tgtactttct
    10681 taggaaaatt tttagatagt aaattgaata ctactccagt tctgacaata atatttttat
    10741 tgttgggtgt tggaggtgct tttagtggtg tttataaaac tctaatagta tatacacgaa
    10801 ggaagtgata tattggatat aaaattactt aaccaggtaa ggtcagtaac aaaaggtata
    10861 gtaatatttg atgtagtttt aattgtgcta atgttgataa catcaacatt aagtaaacca
    10921 gtattattag gacttatatt tggctctata atagctatgt taaattttag attattggcg
    10981 atatcactgg aaaaatcagt gacatttcct gctgggaagg cacaagctta tgcatcagca
    11041 caatatgctg ttagattatt tataatggca gttgttctaa ttgcatcagc tacagtacca
    11101 catttaaata taatcggaac agcattggga ttattgagca caaagtttgt catattatca
    11161 aaaaacttta taaataaatt aaagaggaag gaggcataag tatgaatcaa gcgcattatt
    11221 atctcatagg aaactttaag attagtgaaa caatactagt aagttgggga ataatggcct
    11281 ttttagcgat agcatcttat tttttgacta gaaacttaaa aaaagttcca actagtaaag
    11341 ttcaaatatt cttagaattt gcagtaggtg gtcttgctaa aatggttaca gatgttatgg
    11401 gtaaaaatac agtaaaaaga atgccatata tgataccata cataggaagc ttgtttttat
    11461 ttttcgcatg ttcaaattta agcccattac ttggattaag gtcaccaaca accgacttag
    11521 acacaactgc tgcatgggca atgattacat tttttatgat ttattttgca ggagttaaat
    11581 ttaaggggtt atcatacttt aaagaattgg ctcaaccact tgccataatg ttaccaatca
    11641 atattatagg tgagttagct agaccaatat cattaagttt ccgtccattt gggaatattt
    11701 taggtggaac aataataatg ggtctgtttt atcaactttt aggttttata tctagtttaa
    11761 taccaggatt atcaatacca attggagaaa tattaatacc agtcccatta cacttgtact
    11821 ttgacttatt tgcaggattg ttacaagcat atatatttat aatgttaaca atggtattta
    11881 tatcaagtgc aggagagtta gattaagaaa tcaacttata ttaagataaa aataaaataa
    11941 tttaaaaaat taaagtttta aggaggaaat aattatggaa acagcaatag tagcggcagc
    12001 atcagcaata ggagcaggga tagcagttgc aacaggtata ggtgcaggta taggtcaagg
    12061 tatagcagca gctaaagctg cagaagcagt aggaaaccag ccagaagcta aaggtgatat
    12121 aacatcaact ttattactag gagttgctat agcagagtct tcagcaatat atggtttggt
    12181 tatatcaata atacttctat tcgtaaatcc attctttaaa tacttaggaa tgtagattta
    12241 gtccttaaat attttgagat aggtttatag gtaaatcatc tcaaacttat aatcatgaga
    12301 tgaactttat catctcgtga ttataagtag ttatgattta tggaaaggag gataattatg
    12361 gaaaaagcac tagtaggtat aacgtgggaa tttgtgttcc aaattgttaa tacttttata
    12421 atatttttac ttctgagaaa gcttttattc aagccagtat taaatataat tgaatcaaga
    12481 gaaaatgata ttaaaagtga tttggcagaa ggcgaaaaag caaaaaatga aggtttagct
    12541 ctgaaaaaag aatatgagtc aaaaatcaat tttgccaaag atgaaggtca agaaatcata
    12601 aaacaagcaa ctataagagc agagcagaaa tcagatgata tagtaaatac tgcaaagaaa
    12661 gatgctcttg atataaaaga aaaagcaaac aaagatatag aacaagaaag acaaaaggtt
    12721 ataaatgaaa tcaaaaatga tatatctaac atagcactac ttgcagcatc taaagttatt
    12781 gaaaaagatt tagataaatc taaacatgaa gaattgatag aaaactttat aaaagaggta
    12841 ggggaagcta aatgataaat gtaatagcaa atagatatgc cgaagcccta tttcaattag
    12901 gagaagaaga aaattcaact gatgtcttat tcaaagaact agaaaaagta gtagatatga
    12961 tgactaaagt tagtaaagat ttctacaaag ttttaaaatc accattagtt tcaaaatcag
    13021 aaaagaaaaa cttggtagaa attattttta gtaaggaagt aagttctaac ataaaaaact
    13081 ttttaaaagt tttagtagat aaagatagaa tatcatattt agaagatatc gaattagctt
    13141 ataaagaatt attaaataag aaaaacaatg taattgatgg agtagcaata agtgctattc
    13201 caatgagtga aacagatatt aaagaacttg aagtcaaact ttcaaataaa tacaacaaaa
    13261 atgttactat agaaaatgtt gttgacaaga ctatactagg gggagttcta gttagaattg
    13321 gaaatgaaca aatagatggc actgtgaaaa ctcgtcttga taagatgaaa gaaaagctat
    13381 ctgaagtaat atcttaagaa tggcggtgaa cccatgaact taaaacctga agaaataagt
    13441 tctataatta aacagcaaat aaaaaattat gagaataaag ttgagttgac agatacaggt
    13501 agtgttttaa cagttgggga tggtatagcg agtgtatatg gattagaaaa agctatgtct
    13561 ggtgagttgc tagagttccc aggtgaaata tatggtatgg cacttaactt ggaagaagaa
    13621 gtagttggag ctgttatcct tggagatgac agtgaaataa aagaaggcga tatagttaag
    13681 agaactggaa gaatagttga agttccagtt ggagaagctt taataggtag agttgtaaac
    13741 tcattaggtc agccaataga tggaaaagga cctatagcgt acacaaaaac tagaccagtt
    13801 gaatctgagg cacctggtat aatagataga aggtctgttt atgaaccatt acaaacagga
    13861 atcaaatcaa tagattcaat gataccaatt ggtagaggtc aaagagagtt aataataggt
    13921 gacagacaaa ctggtaaaac atctatagtt attgacacta tactaaatca aaaaggaaaa
    13981 gatgttatct gtatatatgt agcaattgga caaaaacgtt ctactatagc tcaattagtt
    14041 agctctttag aaaaaggtgg agctttggat tatacaatag tagtttcagc aacagcatct
    14101 gaatcagcac cacttcaata tatagcacca tatgctggag cagctatggg tgaagaattt
    14161 atgtacaatg ggaaacatgt acttatagtg tatgatgatt taagtaaaca agctgttgca
    14221 tatagagaaa tgtcattgtt acttagaaga ccaccaggac gtgaggcata tcctggagat
    14281 gtattctatt tacattcaag attacttgaa agagcagcta agttatctga tgaattgggt
    14341 ggaggttcaa tgactgcctt gcctataata gaaactcaag ctggtgacgt ttctgcatat
    14401 ataccaacaa atgttatatc tataacagat ggtcaaatat acttacaacc agagttattc
    14461 tattcaggtg taagaccagc agttgaccct ggtatatcag tatcaagggt tggtggttct
    14521 gcgcaaatta aagcaatgaa aaaagttgca ggaacattaa aacttgcata ttcacaatat
    14581 agagaacttg cagcattctc acagtttggt tctgacttag atgaagatac taaaaagaga
    14641 cttgctcaag gtgaaagaat cgttgagata ttaaaacaag gtgagcatca accaataaaa
    14701 gttgaaaatc aagttatgat aatatatgct gttataaata atcatttaga ggatattccg
    14761 atagataata tagctagatt tgaatcagaa ttgtatgcat ttgtagataa taattatccg
    14821 gaaatatcaa gaaaaatatt aggtggagaa gacttcactc atgatttaac agatgcaata
    14881 aatgaattta aagaaaagtt tgttgttgaa gtataatcct ttttaaaaaa ggaggtaaat
    14941 taattggcag gagctggaat caaagcaatc aaaacacgta tgggaactgt tgaaagtaca
    15001 aagcaaataa ctaaagcaat ggaattagtt gcatcttcta agcttagaaa agcaaaagaa
    15061 aaggctgaag attcaagaga atattttaat actttataca atacagtaaa agatattgcc
    15121 caaacaacac gtggaattag aaatgaattt ttaaaagaaa gagaagtaaa aaataaatgc
    15181 tatgtagtag tagctggaga cagaggactt gcaggtggat acaattctaa tgtattaaag
    15241 gaatctgtct ctcatatgaa aggtaaaaag gaaacagtga ttactgttgg aaagaaagca
    15301 catgaatttt tttctaaaag aaactacaat atagcaaaag atataaatag tgttgaagct
    15361 tgtggatatg aagatgcgac agaaatttca caaactatta ttgacctgta taaaaaaggt
    15421 gaagtagatg aagtttatat tgcttataca aaatttaaat ctgctttatc acaagaagtt
    15481 gaaattttga aattgcttcc attagtcttt gaaaaagata cagacgaaca aggaatggca
    15541 gtaaaagaag aaaaacaaaa tagatcaaaa gtacaatata taccatcagc agaagcagtt
    15601 ttaagttata ttgtacctca gtttatatca ggtacagtat atggtggaat tttggaatct
    15661 tttgcatcag agcaaggtgc tagaagaact gcaatggaat cagccacaga caatgcgaat
    15721 gaaatgctat ctaatttaga gttaagttat aacagagcaa gacagtcagc tgtaactcaa
    15781 gaaataacag agatagttgg tggagttgaa gctctaaaat agggaggtta ggaaatggca
    15841 aatgtaggta aagtagtaca gattgtagga gctgtactag atgttaagtt tgatggtgaa
    15901 caaagcctac ctaacttatt aaatgcgtta gtaataaagt taggagataa agaaatagtt
    15961 gcagaagttg ctcaacatat aggtgatgat actgtaagat gtatagctat gagtgcaaca
    16021 gacggacttg taagaggtat ggaagttgta gatacaggag ggccaataag tgttcctgtt
    16081 ggagatgaga ctttaggtag aatattcaat gttcttggaa aaccagttga tgggaaacca
    16141 gctccaaaat ctgcacctaa attacctata catagaccag caccggcgta tgatgaacta
    16201 gaaactacag cagagatact agaaactggt attaaggtag ttgacttact agctccatat
    16261 ctaaaaggtg gaaaaatagg tctatttgga ggagcaggag taggaaagac agttctaata
    16321 caagagctta taaacaatat tgctaagcaa catggtggta tttctgtatt ttcaggagta
    16381 ggagaaagaa caagagaagg taacgacctt tatggcgaaa tgagtgagtc tggagttata
    16441 aataaaacag ctctagtatt tggtcaaatg aatgaaccac ctggagcgag aatgagagtt
    16501 gctttaactg gacttacaat ggcagaacat tttagagatg agcaaggaca agacgtttta
    16561 cttttcgttg ataatatatt ccgtttcaca caagctggtt cagaagtttc agcacttcta
    16621 ggacgtatgc catcagctgt tggttatcag ccaacattag ctactgaaat gggtgcactt
    16681 caagagagaa taacatcaac taagaaaggt tcaataacat ctgttcaagc agtatatgta
    16741 ccagcagatg acttaactga cccagcacca gctacaacat tctctcactt agatgcaaaa
    16801 acagttttat caagacaaat atcatcactg ggtatatatc cagcagttga ccctcttgaa
    16861 tctacatcaa ggattcttga ccctagtata gtaggaaaag aacattatga agtagctaga
    16921 ggtgtacaat caatacttca aagatataaa gaacttcaag atataattgc tatacttggt
    16981 atggatgaat tatctgatga agataagtta atagttgcac gtgctagaaa gatacaaaga
    17041 ttcttatctc aatcattcac agttgctgag caatttacag gaaatccagg tcaatatgta
    17101 cctgttaaag agactgtaag aggatttaaa gaaattcttg aagggaaaca tgatgactta
    17161 ccagagtcag cattcttatt tgtaggaaca atagaagacg ctgttagaaa agcgaaggga
    17221 agtatgtagt catggccagt gaattttcat tgaaagttgt aactccaagt aatacttttt
    17281 atgatggaga tgttgaaatg atagtagtta gaactacaaa aggagacaaa ggggtgttga
    17341 aaggtcatat accatttgtt tcaggtatac ctgtaggaac tttaaagata aaaaaagagg
    17401 gaagttttaa agaggcgaaa atagcagaag gattcattaa tgtaactcaa gataaaactg
    17461 tcatattaac agaatctgct gagtggattt aataaattaa tttagagtat aaaaagtcat
    17521 tttgaccaaa aaaacttttg ggtaaaatgg cttttttgtg ttttggtgtt aataaaataa
    17581 cgataaaatt gtaagttttt agtgataata aattcacaaa atgtttaaat ttactgccaa
    17641 attatgttgt aattaataag ataaagaaga tttagatagc ttttatgtaa gtgaa
//
LOCUS       DAELLV010000057        17666 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.057, whole genome shotgun sequence.
ACCESSION   DAELLV010000057 DAELLV010000000
VERSION     DAELLV010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17666
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.057"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            75..629
                     /gene="tcdR"
                     /locus_tag="KPD58_001594"
     CDS             75..629
                     /gene="tcdR"
                     /locus_tag="KPD58_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin sigma factor TcdR"
                     /protein_id="HBG0265371.1"
                     /translation="MQKSFYELIVLARNNSVDDLQEILFMFKPLVKKLSRVLHYEEGE
                     TDLIIFFIELIKNIKLSSFSEKSDAIIVKYIHKSLLNKTFELSRRYSKMKFNFVEFDE
                     NILNMKNNYQSKSVFEEDICFFEYILKELSGIQRKVIFYKYLKGYSDREISVKLKISR
                     QAVNKAKNRAFKKIKKDYENYFNL"
     gene            947..8047
                     /gene="tcdB"
                     /locus_tag="KPD58_001595"
                     /gene_synonym="toxB"
     CDS             947..8047
                     /gene="tcdB"
                     /locus_tag="KPD58_001595"
                     /gene_synonym="toxB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_074049122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdB"
                     /protein_id="HBG0265372.1"
                     /translation="MSLVNRKQLEKMANVRFRTQEDEYVAILDALEEYHNMSENTVVE
                     KYLKLKDINSLTDIYIDTYKKSGRNKALKKFKEYLVTEVLELKNNNLTPVEKNLHFVW
                     IGGQINDTAINYINQWKDVNSDYNVNVFYDSNAFLINTLKKTVVESAINDTLESFREN
                     LNDPRFDYNKFFRKRMEIIYDKQKNFINYYKAQREENPELIIDDIVKTYLSNEYSKEI
                     DELNTYIEESLNKITQNSGNDVRNFEEFKNGESFNLYEQELVERWNLAAASDILRISA
                     LKEIGGMYLDVDMLPGIQPDLFESIEKPSSVTVDFWEMTKLEAIMKYKEYIPEYTSEH
                     FDMLDEEVQSSFESVLASKSDKSEIFSSLGDMEASPLEVKIAFNSKGIINQGLISVKD
                     SYCSNLIVKQIENRYKILNNSLNPAISEDNDFNTTTNTFIDSIMAEANADNGRFMMEL
                     GKYLRVGFFPDVKTTINLSGPEAYAAAYQDLLMFKEGSMNIHLIEADLRNFEISKTNI
                     SQSTEQEMASLWSFDDARAKAQFEEYKRNYFEGSLGEDDNLDFSQNIVVDKEYLLEKI
                     SSLARSSERGYIHYIVQLQGDKISYEAACNLFAKTPYDSVLFQKNIEDSEIAYYYNPG
                     DGEIQEIDKYKIPSIISDRPKIKLTFIGHGKDEFNTDIFAGFDVDSLSTEIEAAIDLA
                     KEDISPKSIEINLLGCNMFSYSINVEETYPGKLLLKVKDKISELMPSISQDSIIVSAN
                     QYEVRINSEGRRELLDHSGEWINKEESIIKDISSKEYISFNPKENKITVKSKNLPELS
                     TLLQEIRNNSNSSDIELEEKVMLTECEINVISNIDTQIVEERIEEAKNLTSDSINYIK
                     DEFKLIESISDALCDLKQQNELEDSHFISFEDISETDEGFSIRFINKETGESIFVETE
                     KTIFSEYANHITEEISKIKGTIFDTVNGKLVKKVNLDTTHEVNTLNAAFFIQSLIEYN
                     SSKESLSNLSVAMKVQVYAQLFSTGLNTITDAAKVVELVSTALDETIDLLPTLSEGLP
                     IIATIIDGVSLGAAIKELSETSDPLLRQEIEAKIGIMAVNLTTATTAIITSSLGIASG
                     FSILLVPLAGISAGIPSLVNNELVLRDKATKVVDYFKHVSLVETEGVFTLLDDKIMMP
                     QDDLVISEIDFNNNSIVLGKCEIWRMEGGSGHTVTDDIDHFFSAPSITYREPHLSIYD
                     VLEVQKEELDLSKDLMVLPNAPNRVFAWETGWTPGLRSLENDGTKLLDRIRDNYEGEF
                     YWRYFAFIADALITTLKPRYEDTNIRINLDSNTRSFIVPIITTEYIREKLSYSFYGSG
                     GTYALSLSQYNMGINIELSESDVWIIDVDNVVRDVTIESDKIKKGDLIEGILSTLSIE
                     ENKIILNSHEINFSGEVNGSNGFVSLTFSILEGINAIIEVDLLSKSYKLLISGELKIL
                     MLNSNHIQQKIDYIGFNSELQKNIPYSFVDSEGKENGFINGSTKEGLFVSELPDVVLI
                     SKVYMDDSKPSFGYYSNNLKDVKVITKDNVNILTGYYLKDDIKISLSLTLQDEKTIKL
                     NSVHLDESGVAEILKFMNRKGNTNTSDSLMSFLESMNIKSIFVNFLQSNIKFILDANF
                     IISGTTSIGQFEFICDENDNIQPYFIKFNTLETNYTLYVGNRQNMIVEPNYDLDDSGD
                     ISSTVINFSQKYLYGIDSCVNKVVISPNIYTDEINITPVYETNNTYPEVIVLDANYIN
                     EKINVNINDLSIRYVWSNDGNDFILMSTSEENKVSQVKIRFVNVFKDKTLANKLSFNF
                     SDKQDVPVSEIILSFTPSYYEDGLIGYDLGLVSLYNEKFYINNFGMMLSGLIYINDSL
                     YYFKPPVNNLITGFVTVGDDKYYFNPINGGAASIGETIIDDKNYYFNQSGVLQTGVFS
                     TEDGFKYFAPANTLDENLEGEAIDFTGKLIIDENIYYFDDNYRGAVEWKELDGEMHYF
                     SPETGKAFKGLNQIGDDKYYFNSDGVMQKGFVSINDNKHYFDDSGVMKVGYTEIDGKH
                     FYFAENGEMQIGVFNTEDGFKYFAHHNEDLGNEEGEEISYSGILNFNNKIYYFDDSFT
                     AVVGWKDLEDGSKYYFDEDTAEAYIGLSLINDGQYYFNDDGIMQVGFVTINDKVFYFS
                     DSGIIESGVQNIDDNYFYIDDNGIVQIGVFDTSDGYKYFAPANTVNDNIYGQAVEYSG
                     LVRVGEDVYYFGETYTIETGWIYDMENESDKYYFNPETKKACKGINLIDDIKYYFDEK
                     GIMRTGLISFENNNYYFNENGEMQFGYINIEDKMFYFGEDGVMQIGVFNTPDGFKYFA
                     HQNTLDENFEGESINYTGWLDLDEKRYYFTDEYIAATGSVIIDGEEYYFDPDTAQLVI
                     SE"
     gene            8169..8669
                     /gene="tcdE"
                     /locus_tag="KPD58_001596"
     CDS             8169..8669
                     /gene="tcdE"
                     /locus_tag="KPD58_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like glycosylating toxin export protein
                     TcdE"
                     /protein_id="HBG0265373.1"
                     /translation="MHSSSPFYISNGNKIFFYINLGGVMNMTISFLSEHIFIKLVILT
                     ISFDTLLGCLSAIKSRKFNSSFGIDGGIRKVAMIACIFFLSVVDILTKFNFLFMLPQD
                     CINFLRLKHLGISEFFSILFILYESVSILKNMCLCGLPVPKRLKEKIAILLDAMTDEM
                     NAKDEK"
     gene            8748..8909
                     /locus_tag="KPD58_001597"
     CDS             8748..8909
                     /locus_tag="KPD58_001597"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265374.1"
                     /translation="MKLIMKTREQMPRDTQVLNTYNFEASVHYYIDDKVVYQTLVHKD
                     GAWSVGKIY"
     gene            8998..9180
                     /locus_tag="KPD58_001598"
     CDS             8998..9180
                     /locus_tag="KPD58_001598"
                     /inference="COORDINATES: protein motif:HMM:NF022413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265375.1"
                     /translation="MRDEGQNDIENKYQTLINDLIDSLKVIGKIYKEIKTLKKYKICL
                     QITIRQSPYLIEESIN"
     gene            9397..17529
                     /gene="tcdA"
                     /locus_tag="KPD58_001599"
                     /gene_synonym="toxA"
     CDS             9397..17529
                     /gene="tcdA"
                     /locus_tag="KPD58_001599"
                     /gene_synonym="toxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_105161403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdA"
                     /protein_id="HBG0265376.1"
                     /translation="MSLISKEELIKLAYSIRPRENEYKTILTNLDEYNKLTTNNNENK
                     YLQLKKLNESIDVFMNKYKTSSRNRALSNLKKDILKEVILIKNSNTSPVEKNLHFVWI
                     GGEVSDIALEYIKQWADINAEYNIKLWYDSEAFLVNTLKKAIVESSTTEALQLLEEEI
                     QNPQFDNMKFYKKRMEFIYDRQKRFINYYKSQINKPTVPTIDDIIKSHLVSEYNRDET
                     VLESYRTNSLRKINSNHGIDIRANSLFTEQELLNIYSQELLNRGNLAAASDIVRLLAL
                     KNFGGVYLDVDMLPGIHSDLFKTISRPSSIGLDRWEMIKLEAIMKYKKYINNYTSENF
                     DKLDQQLKDNFKLIIESKSEKSEIFSKLENLNVSDLEIKIAFALGSVINQALISKQGS
                     YLTNLVIEQVKNRYQFLNQHLNPAIESDNNFTDTTKIFHDSLFNSATAENSMFLTKIA
                     PYLQVGFMPEARSTISLSGPGAYASAYYDFINLQENTIEKTLKASDLIEFKFPENNLS
                     QLTEQEINSLWSFDQASAKYQFEKYVRDYTGGSLSEDNGVDFNKNTALDKNYLLNNKI
                     PSNNVEEAGSKNYVHYIIQLQGDDISYEATCNLFSKNPKNSIIIQRNMNESAKSYFLS
                     DDGESILELNKYRIPERLKNKEKVKVTFIGHGKDEFNTSEFARLSVDSLSNEISSFLD
                     TIKLDISPKNVEVNLLGCNMFSYDFNVEETYPGKLLLSIMDKITSTLPDVNKNSITIG
                     ANQYEVRINSEGRKELLAHSGKWINKEEAIMSDLSSKEYIFFDSIDNKLKAKSKNIPG
                     LASISEDIKTLLLDASVSPDTKFILNNLKLNIESSIGDYIYYEKLEPVKNIIHNSIDD
                     LIDEFNLLENVSDELYELKKLNNLDEKYLISFEDISKNNSTYSVRFINKSNGESVYVE
                     TEKEIFSKYSEHITKEISTIKNSIITDVNGNLLDNIQLDHTSQVNTLNAAFFIQSLID
                     YSSNKDVLNDLSTSVKVQLYAQLFSTGLNTIYDSIQLVNLISNAVNDTINVLPTITEG
                     IPIVSTILDGINLGAAIKELLDEHDPLLKKELEAKVGVLAINMSLSIAATVASIVGIG
                     AEVTIFLLPIAGISAGIPSLVNNELILHDKATSVVNYFNHLSESKKYGPLKTEDDKIL
                     VPIDDLVISEIDFNNNSIKLGTCNILAMEGGSGHTVTGNIDHFFSSPSISSHIPSLSI
                     YSAIGIETENLDFSKKIMMLPNAPSRVFWWETGAVPGLRSLENDGTRLLDSIRDLYPG
                     KFYWRFYAFFDYAITTLKPVYEDTNIKIKLDKDTRNFIMPTITTNEIRNKLSYSFDGA
                     GGTYSLLLSSYPISTNINLSKDDLWIFNIDNEVREISIENGTIKKGKLIKDVLSKIDI
                     NKNKLIIGNQTIDFSGDIDNKDRYIFLTCELDDKISLIIEINLVAKSYSLLLSGDKNY
                     LISNLSNTIEKINTLGLDSKNIAYNYTDESNNKYFGAISKTSQKSIIHYKKDSKNILE
                     FYNDSTLEFNSKDFIAEDINVFMKDDINTITGKYYVDNNTDKSIDFSISLVSKNQVKV
                     NGLYLNESVYSSYLDFVKNSDGHHNTSNFMNLFLDNISFWKLFGFENINFVIDKYFTL
                     VGKTNLGYVEFICDNNKNIDIYFGEWKTSSSKSTIFSGNGRNVVVEPIYNPDTGEDIS
                     TSLDFSYEPLYGIDRYINKVLIAPDLYTSLININTNYYSNEYYPEIIVLNPNTFHKKV
                     NINLDSSSFEYKWSTEGSDFILVRYLEESNKKILQKIRIKGILSNTQSFNKMSIDFKD
                     IKKLSLGYIMSNFKSFNSENELDRDHLGFKIIDNKTYYYDEDSKLVKGLININNSLFY
                     FDPIEFNLVTGWQTINGKKYYFDINTGAALTSYKIINGKHFYFNNDGVMQLGVFKGPD
                     GFEYFAPANTQNNNIEGQAIVYQSKFLTLNGKKYYFDNDSKAVTGWRIINNEKYYFNP
                     NNAIAAVGLQVIDNNKYYFNPDTAIISKGWQTVNGSRYYFDTDTAIAFNGYKTIDGKH
                     FYFDSDCVVKIGVFSTSNGFEYFAPANTYNNNIEGQAIVYQSKFLTLNGKKYYFDNNS
                     KAVTGWQTIDSKKYYFNTNTAEAATGWQTIDGKKYYFNTNTAEAATGWQTIDGKKYYF
                     NTNTAIASTGYTIINGKHFYFNTDGIMQIGVFKGPNGFEYFAPANTDANNIEGQAILY
                     QNEFLTLNGKKYYFGSDSKAVTGWRIINNKKYYFNPNNAIAAIHLCTINNDKYYFSYD
                     GILQNGYITIERNNFYFDANNESKMVTGVFKGPNGFEYFAPANTHNNNIEGQAIVYQN
                     KFLTLNGKKYYFDNDSKAVTGWQTIDGKKYYFNLNTAEAATGWQTIDGKKYYFNLNTA
                     EAATGWQTIDGKKYYFNTNTFIASTGYTSINGKHFYFNTDGIMQIGVFKGPNGFEYFA
                     PANTDANNIEGQAILYQNKFLTLNGKKYYFGSDSKAVTGLRTIDGKKYYFNTNTAVAV
                     TGWQTINGKKYYFNTNTSIASTGYTIISGKHFYFNTDGIMQIGVFKGPDGFEYFAPAN
                     TDANNIEGQAIRYQNRFLYLHDNIYYFGNNSKAATGWVTIDGNRYYFEPNTAMGANGY
                     KTIDNKNFYFRNGLPQIGVFKGSNGFEYFAPANTDANNIEGQAIRYQNRFLHLLGKIY
                     YFGNNSKAVTGWQTINGKVYYFMPDTAMAAAGGLFEIDGVIYFFGVDGVKAPGIYG"
BASE COUNT         7050 a         1896 c         2798 g         5922 t
ORIGIN      
        1 atgactgatt taattccaat gttgtcaaaa ttttcgaata aatcatcatt ataaatataa
       61 gagaggatga ttttatgcaa aagtcttttt atgaattaat tgttttagca agaaataact
      121 cagtagatga tttgcaagaa attttattta tgtttaagcc attagtaaaa aaacttagta
      181 gagttttaca ttatgaagag ggagaaacag atttaataat attttttatt gaattaataa
      241 aaaatattaa attaagtagc ttttcagaaa aaagcgatgc tattatagtc aaatatattc
      301 ataaatcatt actgaataag acttttgagt tgtctagaag atattctaaa atgaagttta
      361 attttgtaga atttgatgaa aatatcttaa atatgaaaaa taattatcaa agtaagtctg
      421 tttttgagga agatatttgt tttttcgaat atattttgaa agaattatct ggtattcaaa
      481 gaaaagttat tttttataaa tatttaaaag gatattctga tagagaaata tcagtgaaat
      541 taaaaatatc tagacaagct gttaataagg ctaaaaatag agcatttaaa aaaataaaaa
      601 aagactatga aaattatttt aacttgtaat taatgagctt aaagaaatat ttacaataga
      661 aatcaaattt tagaattaac tttattgtaa aatcaataac ttaatctaag aatatcttaa
      721 tttttatatt ttatatagaa caaagtttac atatttattt cagacaacgt ctttattcaa
      781 tcgaagagca aattaatcaa ctgagtgtct tcaatttaaa atgttaggaa gtgaatgtat
      841 atgaaaacct aagtagatat tagtatattt tataaataga aaggaggata tataaaagag
      901 ttttatcatt tagatgtaaa aatattatag taaaggagaa aattttatga gtttagttaa
      961 tagaaaacag ttagaaaaaa tggcaaatgt aagatttcgt actcaagaag atgaatatgt
     1021 tgcaatattg gatgctttag aagaatatca taatatgtca gagaatactg tagtcgaaaa
     1081 atatttaaaa ttaaaagata taaatagttt aacagatatt tatatagata catataaaaa
     1141 atctggtaga aataaagcct taaaaaaatt taaggaatat ctagttacag aagtattaga
     1201 gctaaagaat aataatttaa ctccagttga gaaaaattta cattttgttt ggattggagg
     1261 tcaaataaat gacactgcta ttaattatat aaatcaatgg aaagatgtaa atagtgatta
     1321 taatgttaat gttttttatg atagtaatgc atttttgata aacacattga aaaaaactgt
     1381 agtagaatca gcaataaatg atacacttga atcatttaga gaaaacttaa atgaccctag
     1441 atttgactat aataaattct tcagaaaacg tatggaaata atttatgata aacagaaaaa
     1501 tttcataaac tactataaag ctcaaagaga agaaaatcct gaacttataa ttgatgatat
     1561 tgtaaagaca tatctttcaa atgagtattc aaaggagata gatgaactta atacctatat
     1621 tgaagaatcc ttaaataaaa ttacacagaa tagtggaaat gatgttagaa actttgaaga
     1681 atttaaaaat ggagagtcat tcaacttata tgaacaagag ttggtagaaa ggtggaattt
     1741 agctgctgct tctgacatat taagaatatc tgcattaaaa gaaattggtg gtatgtattt
     1801 agatgttgat atgttaccag gaatacaacc agacttattt gagtctatag agaaacctag
     1861 ttcagtaaca gtggattttt gggaaatgac aaagttagaa gctataatga aatacaaaga
     1921 atatatacca gaatatacct cagaacattt tgacatgtta gacgaagaag ttcaaagtag
     1981 ttttgaatct gttctagctt ctaagtcaga taaatcagaa atattctcat cacttggtga
     2041 tatggaggca tcaccactag aagttaaaat tgcatttaat agtaagggta ttataaatca
     2101 agggctaatt tctgtgaaag actcatattg tagcaattta atagtaaaac aaatcgagaa
     2161 tagatataaa atattgaata atagtttaaa tccagctatt agcgaggata atgattttaa
     2221 tactacaacg aataccttta ttgatagtat aatggctgaa gctaatgcag ataatggtag
     2281 atttatgatg gaactaggaa agtatttaag agttggtttc ttcccagatg ttaaaactac
     2341 tattaactta agtggccctg aagcatatgc ggcagcttat caagatttat taatgtttaa
     2401 agaaggcagt atgaatatcc atttgataga agctgattta agaaactttg aaatctctaa
     2461 aactaatatt tctcaatcaa ctgaacaaga aatggctagc ttatggtcat ttgacgatgc
     2521 aagagctaaa gctcaatttg aagaatataa aaggaattat tttgaaggtt ctcttggtga
     2581 agatgataat cttgattttt ctcaaaatat agtagttgac aaggagtatc ttttagaaaa
     2641 aatatcttca ttagcaagaa gttcagagag aggatatata cactatattg ttcagttaca
     2701 aggagataaa attagttatg aagcagcatg taacttattt gcaaagactc cttatgatag
     2761 tgtactgttt cagaaaaata tagaagattc agaaattgca tattattata atcctggaga
     2821 tggtgaaata caagaaatag acaagtataa aattccaagt ataatttctg atagacctaa
     2881 gattaaatta acatttattg gtcatggtaa agatgaattt aatactgata tatttgcagg
     2941 ttttgatgta gattcattat ccacagaaat agaagcagca atagatttag ctaaagagga
     3001 tatttctcct aagtcaatag aaataaattt attaggatgt aatatgttta gctactctat
     3061 caacgtagag gagacttatc ctggaaaatt attacttaaa gttaaagata aaatatcaga
     3121 attaatgcca tctataagtc aagactctat tatagtaagt gcaaatcaat atgaagttag
     3181 aataaatagt gaaggaagaa gagaattatt agaccattct ggtgaatgga taaacaaaga
     3241 agaaagtatt ataaaggata tttcatcaaa agaatatata tcatttaatc ctaaagaaaa
     3301 taaaattaca gtaaaatcta aaaatttacc tgagctatct acattattac aagaaattag
     3361 aaataattct aattcaagtg atattgaact agaagaaaaa gtaatgttaa cagaatgtga
     3421 gataaatgtt atttcaaata tagatacgca aattgttgag gaaaggattg aagaagctaa
     3481 gaatttaact tctgactcta ttaattatat aaaagatgaa tttaaactaa tagaatctat
     3541 ttctgatgca ctatgtgact taaaacaaca gaatgaatta gaagattctc attttatatc
     3601 ttttgaggac atatcagaga ctgatgaggg atttagtata agatttatta ataaagaaac
     3661 tggagaatct atatttgtag aaactgaaaa aacaatattc tctgaatatg ctaatcatat
     3721 aactgaagag atttctaaga taaaaggtac tatatttgat actgtaaatg gtaagttagt
     3781 aaaaaaagta aatttagata ctacacacga agtaaatact ttaaatgctg cattttttat
     3841 acaatcatta atagaatata atagttctaa agaatctctt agtaatttaa gtgtagcaat
     3901 gaaagtccaa gtttacgctc aattatttag tactggttta aatactatta cagatgcagc
     3961 caaagttgtt gaattagtat caactgcatt agatgaaact atagacttac ttcctacatt
     4021 atctgaagga ttacctataa ttgcaactat tatagatggt gtaagtttag gtgcagcaat
     4081 caaagagcta agtgaaacga gtgacccatt attaagacaa gaaatagaag ctaagatagg
     4141 tataatggca gtaaatttaa caacagctac aactgcaatc attacttcat ctttggggat
     4201 agctagtgga tttagtatac ttttagttcc tttagcagga atttcagcag gtataccaag
     4261 cttagtaaac aatgaacttg tacttcgaga taaggcaaca aaggttgtag attattttaa
     4321 acatgtttca ttagttgaaa ctgaaggagt atttacttta ttagatgata aaataatgat
     4381 gccacaagat gatttagtga tatcagaaat agattttaat aataattcaa tagttttagg
     4441 taaatgtgaa atctggagaa tggaaggtgg ttcaggtcat actgtaactg atgatataga
     4501 tcacttcttt tcagcaccat caataacata tagagagcca cacttatcta tatatgacgt
     4561 attggaagta caaaaagaag aacttgattt gtcaaaagat ttaatggtat tacctaatgc
     4621 tccaaataga gtatttgctt gggaaacagg atggacacca ggtttaagaa gcttagaaaa
     4681 tgatggcaca aaactgttag accgtataag agataactat gaaggtgagt tttattggag
     4741 atattttgct tttatagctg atgctttaat aacaacatta aaaccaagat atgaagatac
     4801 taatataaga ataaatttag atagtaatac tagaagtttt atagttccaa taataactac
     4861 agaatatata agagaaaaat tatcatattc tttctatggt tcaggaggaa cttatgcatt
     4921 gtctctttct caatataata tgggtataaa tatagaatta agtgaaagtg atgtttggat
     4981 tatagatgtt gataatgttg tgagagatgt aactatagaa tctgataaaa ttaaaaaagg
     5041 tgatttaata gaaggtattt tatctacact aagtattgaa gagaataaaa ttatcttaaa
     5101 tagccatgag attaattttt ctggtgaggt aaatggaagt aatggatttg tttctttaac
     5161 attttcaatt ttagaaggaa taaatgcaat tatagaagtt gatttattat ctaaatcata
     5221 taaattactt atttctggcg aattaaaaat attgatgtta aattcaaatc atattcaaca
     5281 gaaaatagat tatataggat tcaatagcga attacagaaa aatataccat atagctttgt
     5341 agatagtgaa ggaaaagaga atggttttat taatggttca acaaaagaag gtttatttgt
     5401 atctgaatta cctgatgtag ttcttataag taaggtttat atggatgata gtaagccttc
     5461 atttggatat tatagtaata atttgaaaga tgtcaaagtt ataactaaag ataatgttaa
     5521 tatattaaca ggttattatc ttaaggatga tataaaaatc tctctttctt tgactctaca
     5581 agatgaaaaa actataaagt taaatagtgt gcatttagat gaaagtggag tagctgagat
     5641 tttgaagttc atgaatagaa aaggtaatac aaatacttca gattctttaa tgagcttttt
     5701 agaaagtatg aatataaaaa gtattttcgt taatttctta caatctaata ttaagtttat
     5761 attagatgct aattttataa taagtggtac tacttctatt ggccaatttg agtttatttg
     5821 tgatgaaaat gataatatac aaccatattt cattaagttt aatacactag aaactaatta
     5881 tactttatat gtaggaaata gacaaaatat gatagtggaa ccaaattatg atttagatga
     5941 ttctggagat atatcttcaa ctgttatcaa tttctctcaa aagtatcttt atggaataga
     6001 cagttgtgtt aataaagttg taatttcacc aaatatttat acagatgaaa taaatataac
     6061 gcctgtatat gaaacaaata atacttatcc agaagttatt gtattagatg caaattatat
     6121 aaatgaaaaa ataaatgtta atatcaatga tctatctata cgatatgtat ggagtaatga
     6181 tggtaatgat tttattctta tgtcaactag tgaagaaaat aaggtgtcac aagttaaaat
     6241 aagattcgtt aatgttttta aagataagac tttggcaaat aagctatctt ttaactttag
     6301 tgataaacaa gatgtacctg taagtgaaat aatcttatca tttacacctt catattatga
     6361 ggatggattg attggctatg atttgggtct agtttcttta tataatgaga aattttatat
     6421 taataacttt ggaatgatgc tatctggatt aatatatatt aatgattcat tatattattt
     6481 taaaccacca gtaaataatt tgataactgg atttgtgact gtaggcgatg ataaatacta
     6541 ctttaatcca attaatggtg gagctgcttc aattggagag acaataattg atgacaaaaa
     6601 ttattatttc aaccaaagtg gagtgttaca aacaggtgta tttagtacag aagatggatt
     6661 taaatatttt gccccagcta atacacttga tgaaaaccta gaaggagaag caattgattt
     6721 tactggaaaa ttaattattg acgaaaatat ttattatttt gatgataatt atagaggagc
     6781 tgtagaatgg aaagaattag atggtgaaat gcactatttt agcccagaaa caggtaaagc
     6841 ttttaaaggt ctaaatcaaa taggtgatga taaatactat ttcaattctg atggagttat
     6901 gcaaaaagga tttgttagta taaatgataa taaacactat tttgatgatt ctggtgttat
     6961 gaaagtaggt tacactgaaa tagatggcaa gcatttctac tttgctgaaa acggagaaat
     7021 gcaaatagga gtatttaata cagaagatgg atttaaatat tttgctcatc ataatgaaga
     7081 tttaggaaat gaagaaggtg aagaaatctc atattctggt atattaaatt tcaataataa
     7141 aatttactat tttgatgatt catttacagc tgtagttgga tggaaagatt tagaggatgg
     7201 ttcaaagtat tattttgatg aagatacagc agaagcatat ataggtttgt cattaataaa
     7261 tgatggtcaa tattatttta atgatgatgg aattatgcaa gttggatttg tcactataaa
     7321 tgataaagtc ttctacttct ctgactctgg aattatagaa tctggagtac aaaacataga
     7381 tgacaattat ttctatatag atgataatgg tatagttcaa attggtgtat ttgatacttc
     7441 agatggatat aaatattttg cacctgctaa tactgtaaat gataatattt acggacaagc
     7501 agttgaatat agtggtttag ttagagttgg ggaagatgta tattattttg gagaaacata
     7561 tacaattgag actggatgga tatatgatat ggaaaatgaa agtgataaat attatttcaa
     7621 tccagaaact aaaaaagcat gcaaaggtat taatttaatt gatgatataa aatattattt
     7681 tgatgagaag ggcataatga gaacgggtct tatatcattt gaaaataata attattactt
     7741 taatgagaat ggtgaaatgc aatttggtta tataaatata gaagataaga tgttctattt
     7801 tggtgaagat ggtgtcatgc agattggagt atttaataca ccagatggat ttaaatactt
     7861 tgcacatcaa aatactttgg atgagaattt tgagggagaa tcaataaact atactggttg
     7921 gttagattta gatgaaaaga gatattattt tacagatgaa tatattgcag caactggttc
     7981 agttattatt gatggtgagg agtattattt tgatcctgat acagctcaat tagtgattag
     8041 tgaatagata aaaatatgtt aaatatatcc tcttatactt aaatatataa aaataaacaa
     8101 aatgatacac tacataaagt gttctatcta atatgaagat ttaccaataa aaaggtggac
     8161 tatgatgaat gcacagtagt tcaccttttt atatttctaa tggtaacaaa atattttttt
     8221 atataaacct aggaggcgtt atgaatatga caatatcttt tttatcagag catatattta
     8281 taaagttagt aattttaact atatcatttg atacattatt aggatgttta agtgcaataa
     8341 aaagtcgtaa atttaattct agttttggaa tagatggagg aatcagaaaa gtagcaatga
     8401 tagcatgtat atttttttta tcagtagttg acattcttac aaagtttaac tttttattta
     8461 tgttaccaca agattgtatc aattttttaa gactaaaaca tcttggaata tctgaatttt
     8521 tctctatttt atttatttta tatgaaagtg taagtatatt aaaaaatatg tgcttatgtg
     8581 gattaccagt acctaagaga ttaaaggaaa aaatagcaat tttactagat gcaatgacag
     8641 atgaaatgaa tgctaaggat gaaaagtaag taatggtaga tataataaag atattaacaa
     8701 ataaaaagtg ttatccaaat aagaatagct gaaagttatc ataattcatg aaactaataa
     8761 tgaaaacgag ggagcagatg ccaagagaca cacaagtatt aaatacatat aatttcgaag
     8821 caagtgttca ttactatata gatgacaagg tagtatatca aacattggtt cacaaagatg
     8881 gtgcatggtc agttggtaaa atctattaag ctacattagt tacagatatc acaaactata
     8941 atagttaaac atagaaatat gtgtaaattg tgatggaaat tattcaaaaa cacaaaaata
     9001 cgtgatgaag gacaaaatga tatagaaaat aagtatcaaa ccttaataaa tgatttaatt
     9061 gatagtttaa aagttatagg aaaaatatat aaagaaataa aaacattaaa aaaatataag
     9121 atatgtttac aaattactat cagacaatct ccttatctaa tagaagagtc aattaactaa
     9181 ttgagtatct ttaaattgaa atgttaggaa gtgatttaaa tatgaaaact taaattataa
     9241 aaaatcaata ttaatttatt tttaaaaaat agaaaggagt gtataagatt tattttcaaa
     9301 gtttaaaaac aagaaaatca atttaaattt cagaaggaat aaatgtggtt atagaagtgg
     9361 atttattatc aaaaataata atactaggag gtttttatgt ctttaatatc taaagaagag
     9421 ttaataaaac tcgcatatag cattagacca agagaaaatg agtataaaac tatactaact
     9481 aatttagacg aatataataa gttaactaca aacaataatg aaaataaata tttacaatta
     9541 aaaaaactaa atgaatcaat tgatgttttt atgaataaat ataaaacttc aagcagaaat
     9601 agagcactct ctaatctaaa aaaagatata ttaaaagaag taattcttat taaaaattcc
     9661 aatacaagcc ctgtagaaaa aaatttacat tttgtatgga taggtggaga agtcagtgat
     9721 attgctcttg aatacataaa acaatgggct gatattaatg cagaatataa tattaaactg
     9781 tggtatgata gtgaagcatt cttagtaaat acactaaaaa aggctatagt tgaatcttct
     9841 accactgaag cattacagct actagaggaa gagattcaaa atcctcaatt tgataatatg
     9901 aaattttaca aaaaaaggat ggaatttata tatgatagac aaaaaaggtt tataaattat
     9961 tataaatctc aaatcaataa acctacagta cctacaatag atgatattat aaagtctcat
    10021 ctagtatctg aatataatag agatgaaact gtattagaat catatagaac aaattctttg
    10081 agaaaaataa atagtaatca tgggatagat atcagggcta atagtttgtt tacagaacaa
    10141 gagttattaa atatttatag tcaggagttg ttaaatcgtg gaaatttagc tgcagcatct
    10201 gacatagtaa gattattagc cctaaaaaat tttggcggag tatatttaga tgttgatatg
    10261 cttccaggta ttcactctga tttatttaaa acaatatcta gacctagctc tattggacta
    10321 gaccgttggg aaatgataaa attagaggct attatgaagt ataaaaaata tataaataat
    10381 tatacatcag aaaactttga taaacttgat caacaattaa aagataattt taaactcatt
    10441 atagaaagta aaagtgaaaa atctgagata ttttctaaat tagaaaattt aaatgtatct
    10501 gatcttgaaa ttaaaatagc tttcgcttta ggcagtgtta taaatcaagc cttgatatca
    10561 aaacaaggtt catatcttac taacctagta atagaacaag taaaaaatag atatcaattt
    10621 ttaaaccaac accttaaccc agccatagag tctgataata acttcacaga tactactaaa
    10681 atttttcatg attcattatt taattcagct accgcagaaa actctatgtt tttaacaaaa
    10741 atagcaccat acttacaagt aggttttatg ccagaagctc gctccacaat aagtttaagt
    10801 ggtccaggag cttatgcgtc agcttactat gatttcataa atttacaaga aaatactata
    10861 gaaaaaactt taaaagcatc agatttaata gaatttaaat tcccagaaaa taatctatct
    10921 caattgacag aacaagaaat aaatagtcta tggagctttg atcaagcaag tgcaaaatat
    10981 caatttgaga aatatgtaag agattatact ggtggatctc tttctgaaga caatggggta
    11041 gactttaata aaaatactgc cctcgacaaa aactatttat taaataataa aattccatca
    11101 aacaatgtag aagaagctgg aagtaaaaat tatgttcatt atatcataca gttacaagga
    11161 gatgatataa gttatgaagc aacatgcaat ttattttcta aaaatcctaa aaatagtatt
    11221 attatacaac gaaatatgaa tgaaagtgca aaaagctact ttttaagtga tgatggagaa
    11281 tctattttag aattaaataa atataggata cctgaaagat taaaaaataa ggaaaaagta
    11341 aaagtaacct ttattggaca tggtaaagat gaattcaaca caagcgaatt tgctagatta
    11401 agtgtagatt cactttccaa tgagataagt tcatttttag ataccataaa attagatata
    11461 tcacctaaaa atgtagaagt aaacttactt ggatgtaata tgtttagtta tgattttaat
    11521 gttgaagaaa cttatcctgg gaagttgcta ttaagtatta tggacaaaat tacttccact
    11581 ttacctgatg taaataaaaa ttctattact ataggagcaa atcaatatga agtaagaatt
    11641 aatagtgagg gaagaaaaga acttctggct cactcaggta aatggataaa taaagaagaa
    11701 gctattatga gcgatttatc tagtaaagaa tacatttttt ttgattctat agataataag
    11761 ctaaaagcaa agtccaagaa tattccagga ttagcatcaa tatcagaaga tataaaaaca
    11821 ttattacttg atgcaagtgt tagtcctgat acaaaattta ttttaaataa tcttaagctt
    11881 aatattgaat cttctattgg tgattacatt tattatgaaa aattagagcc tgttaaaaat
    11941 ataattcaca attctataga tgatttaata gatgagttca atctacttga aaatgtatct
    12001 gatgaattat atgaattaaa aaaattaaat aatctagatg agaagtattt aatatctttt
    12061 gaagatatct caaaaaataa ttcaacttac tctgtaagat ttattaacaa aagtaatggt
    12121 gagtcagttt atgtagaaac agaaaaagaa attttttcaa aatatagcga acatattaca
    12181 aaagaaataa gtactataaa gaatagtata attacagatg ttaatggtaa tttattggat
    12241 aatatacagt tagatcatac ttctcaagtt aatacattaa acgcagcatt ctttattcaa
    12301 tcattaatag attatagtag caataaagat gtactgaatg atttaagtac ctcagttaag
    12361 gttcaacttt atgctcaact atttagtaca ggtttaaata ctatatatga ctctatccaa
    12421 ttagtaaatt taatatcaaa tgcagtaaat gatactataa atgtactacc tacaataaca
    12481 gaggggatac ctattgtatc tactatatta gacggaataa acttaggtgc agcaattaag
    12541 gaattactag acgaacatga cccattacta aaaaaagaat tagaagctaa ggtgggtgtt
    12601 ttagcaataa atatgtcatt atctatagct gcaactgtag cttcaattgt tggaataggt
    12661 gctgaagtta ctattttctt attacctata gctggtatat ctgcaggaat accttcatta
    12721 gttaataatg aattaatatt gcatgataag gcaacttcag tggtaaacta ttttaatcat
    12781 ttgtctgaat ctaaaaaata tggccctctt aaaacagaag atgataaaat tttagttcct
    12841 attgatgatt tagtaatatc agaaatagat tttaataata attcgataaa actaggaaca
    12901 tgtaatatat tagcaatgga ggggggatca ggacacacag tgactggtaa tatagatcac
    12961 tttttctcat ctccatctat aagttctcat attccttcat tatcaattta ttctgcaata
    13021 ggtatagaaa cagaaaatct agatttttca aaaaaaataa tgatgttacc taatgctcct
    13081 tcaagagtgt tttggtggga aactggagca gttccaggtt taagatcatt ggaaaatgac
    13141 ggaactagat tacttgattc aataagagat ttatacccag gtaaatttta ctggagattc
    13201 tatgcttttt tcgattatgc aataactaca ttaaaaccag tttatgaaga cactaatatt
    13261 aaaattaaac tagataaaga tactagaaac ttcataatgc caactataac tactaacgaa
    13321 attagaaaca aattatctta ttcatttgat ggagcaggag gaacttactc tttattatta
    13381 tcttcatatc caatatcaac gaatataaat ttatctaaag atgatttatg gatatttaat
    13441 attgataatg aagtaagaga aatatctata gaaaatggta ctattaaaaa aggaaagtta
    13501 ataaaagatg ttttaagtaa aattgatata aataaaaata aacttattat aggcaatcaa
    13561 acaatagatt tttcaggcga tatagataat aaagatagat atatattctt gacttgtgag
    13621 ttagatgata aaattagttt aataatagaa ataaatcttg ttgcaaaatc ttatagtttg
    13681 ttattgtctg gggataaaaa ttatttgata tccaatttat ctaatactat tgagaaaatc
    13741 aatactttag gcctagatag taaaaatata gcgtacaatt acactgatga atctaataat
    13801 aaatattttg gagctatatc taaaacaagt caaaaaagca taatacatta taaaaaagac
    13861 agtaaaaata tattagaatt ttataatgac agtacattag aatttaacag taaagatttt
    13921 attgctgaag atataaatgt atttatgaaa gatgatatta atactataac aggaaaatac
    13981 tatgttgata ataatactga taaaagtata gatttctcta tttctttagt tagtaaaaat
    14041 caagtaaaag taaatggatt atatttaaat gaatccgtat actcatctta ccttgatttt
    14101 gtgaaaaatt cagatggaca ccataatact tctaatttta tgaatttatt tttggacaat
    14161 ataagtttct ggaaattgtt tgggtttgaa aatataaatt ttgtaatcga taaatacttt
    14221 acccttgttg gtaaaactaa tcttggatat gtagaattta tttgtgacaa taataaaaat
    14281 atagatatat attttggtga atggaaaaca tcgtcatcta aaagcactat atttagcgga
    14341 aatggtagaa atgttgtagt agagcctata tataatcctg atacgggtga agatatatct
    14401 acttcactag atttttccta tgaacctctc tatggaatag atagatatat caataaagta
    14461 ttgatagcac ctgatttata tacaagttta ataaatatta ataccaatta ttattcaaat
    14521 gagtactacc ctgagattat agttcttaac ccaaatacat tccacaaaaa agtaaatata
    14581 aatttagata gttcttcttt tgagtataaa tggtctacag aaggaagtga ctttatttta
    14641 gttagatact tagaagaaag taataaaaaa atattacaaa aaataagaat caaaggtatc
    14701 ttatctaata ctcaatcatt taataaaatg agtatagatt ttaaagatat taaaaaacta
    14761 tcattaggat atataatgag taattttaaa tcatttaatt ctgaaaatga attagataga
    14821 gatcatttag gatttaaaat aatagataat aaaacttatt actatgatga agatagtaaa
    14881 ttagttaaag gattaatcaa tataaataat tcattattct attttgatcc tatagaattt
    14941 aacttagtaa ctggatggca aactatcaat ggtaaaaaat attattttga tataaatact
    15001 ggagcagctt taactagtta taaaattatt aatggtaaac acttttattt taataatgat
    15061 ggtgtgatgc agttgggagt atttaaagga cctgatggat ttgaatattt tgcacctgcc
    15121 aatactcaaa ataataacat agaaggtcag gctatagttt atcaaagtaa attcttaact
    15181 ttgaatggca aaaaatatta ttttgataat gactcaaaag cagtcactgg atggagaatt
    15241 attaacaatg agaaatatta ctttaatcct aataatgcta ttgctgcagt cggattgcaa
    15301 gtaattgaca ataataagta ttatttcaat cctgacactg ctatcatctc aaaaggttgg
    15361 cagactgtta atggtagtag atactacttt gatactgata ccgctattgc ctttaatggt
    15421 tataaaacta ttgatggtaa acacttttat tttgatagtg attgtgtagt gaaaataggt
    15481 gtgtttagta cctctaatgg atttgaatat tttgcacctg ctaatactta taataataac
    15541 atagaaggtc aggctatagt ttatcaaagt aaattcttaa ctttgaatgg taaaaaatat
    15601 tactttgata ataactcaaa agcagttacc ggatggcaaa ctattgatag taaaaaatat
    15661 tactttaata ctaacactgc tgaagcagct actggatggc aaactattga tggtaaaaaa
    15721 tattacttta atactaacac tgctgaagca gctactggat ggcaaactat tgatggtaaa
    15781 aaatattact ttaatactaa cactgctata gcttcaactg gttatacaat tattaatggt
    15841 aaacattttt attttaatac tgatggtatt atgcagatag gagtgtttaa aggacctaat
    15901 ggatttgaat attttgcacc tgctaatacg gatgctaaca acatagaagg tcaagctata
    15961 ctttaccaaa atgaattctt aactttgaat ggtaaaaaat attactttgg tagtgactca
    16021 aaagcagtta ctggatggag aattattaac aataagaaat attactttaa tcctaataat
    16081 gctattgctg caattcatct atgcactata aataatgaca agtattactt tagttatgat
    16141 ggaattcttc aaaatggata tattactatt gaaagaaata atttctattt tgatgctaat
    16201 aatgaatcta aaatggtaac aggagtattt aaaggaccta atggatttga gtattttgca
    16261 cctgctaata ctcacaataa taacatagaa ggtcaggcta tagtttacca gaacaaattc
    16321 ttaactttga atggcaaaaa atattatttt gataatgact caaaagcagt tactggatgg
    16381 caaaccattg atggtaaaaa atattacttt aatcttaaca ctgctgaagc agctactgga
    16441 tggcaaacta ttgatggtaa aaaatattac tttaatctta acactgctga agcagctact
    16501 ggatggcaaa ctattgatgg taaaaaatat tactttaata ctaacacttt catagcctca
    16561 actggttata caagtattaa tggtaaacat ttttatttta atactgatgg tattatgcag
    16621 ataggagtgt ttaaaggacc taatggattt gaatactttg cacctgctaa tacggatgct
    16681 aacaacatag aaggtcaagc tatactttac caaaataaat tcttaacttt gaatggtaaa
    16741 aaatattact ttggtagtga ctcaaaagca gttaccggat tgcgaactat tgatggtaaa
    16801 aaatattact ttaatactaa cactgctgtt gcagttactg gatggcaaac tattaatggt
    16861 aaaaaatact actttaatac taacacttct atagcttcaa ctggttatac aattattagt
    16921 ggtaaacatt tttattttaa tactgatggt attatgcaga taggagtgtt taaaggacct
    16981 gatggatttg aatactttgc acctgctaat acagatgcta acaatataga aggtcaagct
    17041 atacgttatc aaaatagatt cctatattta catgacaata tatattattt tggtaataat
    17101 tcaaaagcgg ctactggttg ggtaactatt gatggtaata gatattactt cgagcctaat
    17161 acagctatgg gtgcgaatgg ttataaaact attgataata aaaattttta ctttagaaat
    17221 ggtttacctc agataggagt gtttaaaggg tctaatggat ttgaatactt tgcacctgct
    17281 aatacggatg ctaacaatat agaaggtcaa gctatacgtt atcaaaatag attcctacat
    17341 ttacttggaa aaatatatta ctttggtaat aattcaaaag cagttactgg atggcaaact
    17401 attaatggta aagtatatta ctttatgcct gatactgcta tggctgcagc tggtggactt
    17461 ttcgagattg atggtgttat atatttcttt ggtgttgatg gagtaaaagc ccctgggata
    17521 tatggctaaa atatatgttt gataaaaaat tattcctgtg ctactaagaa attattttta
    17581 tataataaat attgagattt aattaaaagt catgtgttat tgtaatacat gacttttagt
    17641 taaaattttt ctatcattta ataatc
//
LOCUS       DAELLV010000058        17476 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.058, whole genome shotgun sequence.
ACCESSION   DAELLV010000058 DAELLV010000000
VERSION     DAELLV010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17476
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.058"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1189
                     /locus_tag="KPD58_001600"
     CDS             <1..1189
                     /locus_tag="KPD58_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0265377.1"
                     /translation="EVIKFNNKYLNVNKYNIESFGRVTGYYFCIQEITYIRKLEQNLS
                     KKLREKGQVARYTFDDIKTVSTKMERTKKLGMKIAESDYTILITGESGTGKELMAQSI
                     HNASPRHSQPFIAINCAAMPENLLESELFGYEEGAFTGALKGGKKGLFEQANNGTIFL
                     DEIGDMPIYLQSKLLRVLQENQVMRVGGENVIDIDVRVIAATNKNLLEMIKGDRFRAD
                     LYYRINVLPINIPPLRERKEDIEVMLKHFMKRKRAISQDVQDIINAYEWPGNIRELKN
                     TAMYINIMSSEDVILINDLPHNLLNITEDFSKEISILKDRTSIEKVKLVMECLKNANN
                     LNKNIGRNGIVNSINELGYNITEGEVRGILNMLKELDLIICESGRKGSELNRRGKNIL
                     NSI"
     gene            1433..2179
                     /locus_tag="KPD58_001601"
     CDS             1433..2179
                     /locus_tag="KPD58_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBG0265378.1"
                     /translation="MKKLAKKKYPIIGISGNLLIDEGGMFPGYERAYVNNDYIQSVVM
                     CKAIPYIVPIVYDDEIIKEQVSNIDALILSGGQDVNPLIWKEEPHNKLGAISPKRDSF
                     DMKLLKHALDMKKPVLGICRGEQIINVTEGGSLYQDLSLIEGAYIKHNQQHLSNVPTH
                     TVQIKEGTKLYEILGEKEVLVNSFHHLVVNKVAPGYIVSATSKDGLIEAIEKEGSEFV
                     IGIQWHPEMMTRDYDNMKKIFMAIVKEASK"
     gene            2220..3539
                     /locus_tag="KPD58_001602"
     CDS             2220..3539
                     /locus_tag="KPD58_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG0265379.1"
                     /translation="MEKGIKLGFWSIVLLGINAIIGSGIFGLPGDAYTDIGPASILVL
                     VFCMLLAVSIALCFAEAGSWFDTDGGPYLYAKEAFGDFVGFEVGFMKWIVSMIAWATM
                     ANFFAVTLSSVWPQVAEPLIKNIIIGILVVGLGIINFMGMKQSKHLNNIMTIGKLLPI
                     VLFIAVGLFFIKGSNFTPFVIIQKGQSASSAFVAVSITLFYAFTGFESLAVAAKDMEN
                     PKKNVPKALVMVMFVVSVIYMLILGISIGVLGNGLAGSATPVADAAIKMLGPIGGYII
                     TIGTIVSVGGINIASSIFTPRSAAALVEQGLMPKSIRKTNKNGSPYIAIIVSVIGTLL
                     IAWSGSFTTLSQISVVSRFIQYIPTCLAVLILRKKYAGKDVNFRIPGGAIIPIFAVII
                     SILLLIKAGIDEPMKIVWGLGGMIIVVPIYFYMTKVYSKKYNDVEVK"
     gene            complement(3635..5302)
                     /locus_tag="KPD58_001603"
     CDS             complement(3635..5302)
                     /locus_tag="KPD58_001603"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBG0265380.1"
                     /translation="MKNWWKKATVYQIYPKSFKDSNNDGIGDINGIIEKLDYLYSLGV
                     DLLWLTPMYVSPQRDNGYDIEDYYNIDPKYGTMSDFEKLLKEAHKRDIKIMMDMVLNH
                     TSTEHKWFKESKKSKDNPYRDYYFWKDAKPDGSVPNNWISRFSGTAWKYDETTNQYYL
                     HLFEETQADLNWENEKVREECYKILEFWADKGIDGFRLDVVNLLSKTPGLPDDPITGP
                     KGDGRTHYADGPRIHEYLHNMNQKVFKPKNIVTVGEMSSTTPEECINYTRENREELSM
                     VFSFHHMKTDYKGGAKWTNEMFSLDKLKKAQSDFQYKMYEGKGWNALFYSNHDQPRAL
                     SRFGDDRFFRQESAKMLAITLFGLQGTTYIYQGEEIGMTNAYFTKIDEYDDAESKNAY
                     YHMIKSGIDEKEALLILQQKSRDNARTPMQWDNTIYKGFSNHKPWLKVNNDNISVENE
                     LNDSRSVFYTYQRLIKYRKQYDIFTEGTYRLLDENHKNLFVYEREYKNQNLLVISNFT
                     HDNVVYKLPETYLNKLNYTILETNYTRDIIENKMEIKPYESIMIYYK"
     gene            complement(5499..6221)
                     /locus_tag="KPD58_001604"
     CDS             complement(5499..6221)
                     /locus_tag="KPD58_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0265381.1"
                     /translation="MAKNKFSEIYEVLKEEILDGKYTSNMMLPTELQLIERFSCSRNT
                     VRRAISQLNTEGYVQSIKGKGVVVLENSCSNDFFLNMHNFKGVESIVEDKKVNTATSV
                     LHFSKILIDNKLSKKTGFKVGSEVYYLHRLRYIDNIPKILDINYFLCSIVKDLDVSIA
                     QGSIYKYIEECLGTKIVSSRKIFKIEKATELELKTLPLNDYNCVGVIKNSVYTDDGKL
                     FEYTESKHTPETFVFMDVTQRY"
     gene            6612..8039
                     /locus_tag="KPD58_001605"
     CDS             6612..8039
                     /locus_tag="KPD58_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG0265382.1"
                     /translation="MMQQLSLLGKTMLVPIAVMPAAGILGLLFGPNMLNVPAISNISN
                     IVFSNVDYLFLLGAASAYSKSKDKTSVMVAAVVAYMIFKSNLELLNDTLNAGVFGGII
                     VGACIAWTYNKTYKVKVPAFLSFFAGEKCVITLSPVVALILSYIFSLLWIYPQAAMNQ
                     FGFWLGGAGAIGIFLFLFCNRALIPTGLHQVLNAYILFEMGEYTTSTGEVIRGEIPRF
                     MAGDPNAGFFWTGFYIIMMFSIPAISYAIYKTAQDKKKQEVKGAMTAGALTSFMATVT
                     EPIEFSFLFASPKLYIIHSFYSGLGGVVLYLLGVRLGSFNGASIIDYVLSFSYGDKAW
                     LVIPVGVVFFVLYYVTFKYIIIKDNVQTPGREVEIEIGDKVSEKEKNLKLSHGNFSYM
                     AKQIIKNCGGYENIVTLNNCMTRLRLEVKDATILNDDNIKKTGAKGVIKLSNTSVQII
                     IGTDVVKVKDEMEMQLDELRKQADA"
     gene            8041..8811
                     /locus_tag="KPD58_001606"
     CDS             8041..8811
                     /locus_tag="KPD58_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HBG0265383.1"
                     /translation="MKKLVVRADDIGYSEGVTLGIISSYKNGIVTSAGIMVNMPFSRQ
                     AIEMVKNDDVCFGLHVNLVIGEPCCKEHIKIKNLLDEGGKFVSSKIRRTQLLTGKEVF
                     NYEEVYEEVCAQIETYKLYMSKLPEYIDIHGIEEDVSISAMCDAAKEYGIPSCPYYLD
                     SCISMPSKYPQNDFYKLNKSFVKLFEEKYINLDKDITLMVTHPGFIDYSLIKSSSLIQ
                     ERVYEHAMLCDKEVKMWLKRNNIELVDFKDIFKSNNYI"
     gene            9074..9799
                     /locus_tag="KPD58_001607"
     CDS             9074..9799
                     /locus_tag="KPD58_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG0265384.1"
                     /translation="MEFYERAKKYEYKFTDTEDMIIEYMRKNKEEIVKLSIQSLADKF
                     FTVPNTIVRLCKKMEYDGFSQLKNNLKEELKNEDKEPKNGFESDIKKTLELIDYEKIN
                     KVADIIQKSRSIIVYGIGQNIPLCEILVKEIRGGHDNITYYSQRHEVLHDIGKLGEQD
                     MFFALSLSGETKEVIEAAKTAVNKGCKVVALTHICENQLQQIADISLYCYSTIKQVNG
                     YNISDKVPIMMIMRAIIEQYWLR"
     gene            9929..11854
                     /gene="mngA"
                     /locus_tag="KPD58_001608"
     CDS             9929..11854
                     /gene="mngA"
                     /locus_tag="KPD58_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HBG0265385.1"
                     /translation="MEFKKITNENLVFLKQKIKTKEDAIKFLARKLYYEGKLTSYEEY
                     LEAVMYRETLSPTGFERGLAIPHGKSKSVKEASFAAMTLETPIENWESVDPNNKVELI
                     FLIAVPENEGGNTHIQILSELVTRLSNEDYMNMLLNSKTKKELFNNLDIKEKKEQIKE
                     EIRIQNESKKIILAITACPAGIAHTYMSAEALIKAGKEIGVDVYVEKQGANGMVDPHT
                     PDIIKRADAIIYATDVAPKNTERFEHLPNIKTSVAAPLKRAKEIIYEALEVAQKQGKG
                     DYIEKSSDISYCEKSSWKKDVKSAILTGVSHIIPLIVAGGMILAFAVLASQAFGLQEL
                     YNQEGSWLWQLRKLSGGMLGTLMVPVLSAYMSYSLAEKPGLGPGFAAGLAADLIGSGF
                     LGGMLGGLLTGYIVKFLKKNIPAKGTLAGFVSFWVYPVVGSLVVGLLMLFVVGKPVAA
                     LNNGLINWLNGMTGVNAILLGAIIGAMVSFDLGGPINKAAYTFAIGAIASGNFIPYAI
                     FASVKMVSAFGVTIATVLGRDLFSESEKEIGKQTWILGLAGITEGAIPFMIEDPIRVI
                     PSLVAGSAITGSIVAFFNIGLQVPGAGIFSLALLEGSMSGFIIASIWFFAAVLGAIIS
                     AILLIITRKQKNQRKIH"
     gene            11998..14613
                     /gene="mngB"
                     /locus_tag="KPD58_001609"
     CDS             11998..14613
                     /gene="mngB"
                     /locus_tag="KPD58_001609"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBG0265386.1"
                     /translation="MKKNVHIVPHMHWDREWYFSTEESRILLVNNMEEIMDMLENNHN
                     YPYYVMDGQTAILEDYLAVKPECKERIKKLVQEGRLIIGPWYTQTDEMVVGGESILRN
                     LLYGIKDCDEFGEYMKIGYLPDSFGQSAQMPQILNGFDIKYSMFWRGCSERKGTNKTE
                     FNWKSDDGSSVLVQILPLGYAIGKYLPMNEDELRTRMDKYLPVLDKGATTDHIILPNG
                     HDQMPIQKNIFEVIYKLKECYPERKFFLSRYENIFKELEKNEDIDTIKGEFLDGKYMR
                     VHRSIFSTRMDIKSANARIESKITNILEPLASIAYSLGFEYHHGLIELIWKEIMKNHA
                     HDSIGCCCSDKVHQEIMNRFFLAEEKVDQLITFYKRRIVDAMSCEMALDKLVAFNLMP
                     YDRNEIVSTQVITKMKAFEIFDKDNNKLDFEVVHKEVIDAGLIDRQIVHYGNYDSFMV
                     YTINFKDKIPAMGYKAYMIKEVEHMLEKAYEVCDMVDNDFYTIQVNENGTLKIFDKKL
                     NKTFENVLLMENGGDEGDEYDFSPLPNEKLIFNDNIVANSTIKKNRFNNEIKINYRLK
                     VPKDIQARKNNRADSFIDFDIVINVPNDKAIIDVVFNINNQARDCRVRTYIPTNIASK
                     FSVSDNQFGHIKRSVYDDAMEVWQQEEWSERPDSIYPMLTFVGLSDKEHGVAVLTNST
                     REFEIVGEKFDTIAITLFRSVGFLGKEEMARRPGRPSGIKLPTPDSQMIGDIKIDLAI
                     VTHEKSTLEANVANMAKEYLTKIQTYNKMPYNAMKLNQSEIEVDYSYSLLKETNKDLV
                     LSVVKKAEKEEALVVRFYNSKEETKSANIVFNKNIKTAKWINLNEKELESISIDNNSV
                     NVECLMNQVKTILVK"
     gene            14892..16046
                     /locus_tag="KPD58_001610"
     CDS             14892..16046
                     /locus_tag="KPD58_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBG0265387.1"
                     /translation="MKILISIDSLKGSLSSIEAGNAIKKGILKVKEDAQVKILPLADG
                     GEGTVDALVQGMNGKKETIEVTGPIAKKVDATYGLLKNTSTAIIEIAQASGLTLVPTE
                     LRNPLYTTTYGVGEIIKEAINKGYRNFIVGIGGSATNDAGIGMLQALGFEFYDKNNKL
                     VGLGGKVLNEIRHIKIENRLKELDECKFKIACDVNNPLFGKNGAAYIYGSQKGATSEI
                     IEELDNGLRNFSKVVKNYLSKDVANVEGAGAAGGLGFAFLAFLNSKLESGIKIILEEI
                     KLEEELKDADFVITGEGRLDNQTAMGKAPIGVAKLAKKYEVKVIGLAGATTEDAVKCN
                     EEGIDAYFSIVNRAMTIEEAMDKATASENMTATTTQIFNLITSIQQSKNM"
     gene            complement(16169..>17476)
                     /locus_tag="KPD58_001611"
     CDS             complement(16169..>17476)
                     /locus_tag="KPD58_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0265388.1"
                     /translation="LLHLMITIDRIKKNNYIESVEVVNYIDNNNDISISRDISNFLES
                     EYNITLTSAELYYLVFQLTNKTTVLNYHEMDTKSLPNYIDEHFVKLTKKIIKNVYDLY
                     LIDLSDEEFVVKFTLHVKNLISRAKNNQVLRNQIPQKLKDSYPLIYDISVYICNQIQT
                     LENVDIDEDEISYISLHVGSFFDRQKLLEDKVLCALITPNYYDLQFKIVRDLEKRFNE
                     SIEIIQIFSDTHNLDFDNKVDMVITTLPINNRCPIPFVYVNPYLNRKDYDNIQSKFTQ
                     IKDRKNILTVQNHLEMYFSESLFMKNIYLDSAKDYIKFMGNILYENKYVKPNYIDDVL
                     IREKMSSTAFNNNVAIPHSMKMDALKTGVCLIVNDKPVKWGEEKVQIIAMIPINEKEK
                     EKFNYIFESFIEILSEWNNVKELTKADNYSSFMNRIAYLIQNI"
BASE COUNT         6675 a         2019 c         3045 g         5737 t
ORIGIN      
        1 tgaagttata aaatttaata ataagtactt aaacgtaaat aagtataata tagagagttt
       61 tggaagggtt actggatatt atttttgtat tcaagaaata acttacataa gaaaattgga
      121 gcaaaaccta tcaaagaagc ttagagaaaa gggacaagtt gctagatata cttttgatga
      181 tataaaaaca gtttcaacga agatggaaag aactaagaaa ttaggtatga aaatagctga
      241 atctgattat acaattctta taacgggaga aagtggaaca ggaaaagaac tcatggctca
      301 gtctattcat aatgcatcac caagacatag tcagccattt atagcaataa actgtgcagc
      361 tatgccagaa aatttacttg aaagtgaact ttttggatat gaagaaggtg cttttacagg
      421 agctttaaaa ggaggtaaaa aaggattatt tgaacaggct aacaatggaa ctatattttt
      481 ggatgaaata ggagacatgc ctatatactt acaaagcaaa ttacttagag tattacaaga
      541 gaatcaagta atgagggttg gaggagaaaa tgtaatagat atagatgtta gagtaattgc
      601 ggctacaaat aaaaatttat tagaaatgat aaaaggagat agatttagag cagatttata
      661 ctataggata aatgtattgc ccataaatat accaccttta agagagcgaa aagaagatat
      721 agaagttatg ttaaagcatt ttatgaaaag gaagagagca attagtcaag atgttcaaga
      781 cattattaat gcatatgaat ggccaggtaa tataagagaa ttaaaaaata ctgctatgta
      841 tattaacatt atgagttctg aggatgttat tttaataaat gatttacctc ataacttatt
      901 aaatataact gaagatttta gtaaagaaat aagcatatta aaggatagaa catctataga
      961 gaaagtaaag ttagtaatgg agtgtttaaa aaatgccaat aatcttaata aaaatatagg
     1021 tagaaatggt attgttaatt caataaatga attaggctac aatataacag aaggtgaggt
     1081 aagaggaata cttaatatgt taaaagagct ggatttgata atatgtgaat ctggtagaaa
     1141 gggaagtgaa ttaaatagaa ggggaaaaaa tatattaaac agtatttaat aggttgataa
     1201 aaaggaaaat ctaaatgcct attttaccta ttagttgtac ttaaaacaat ttaaaaaagt
     1261 caaactaaac aggattaaaa taaaatataa gtaaataaag ttatttatat tactcgtatt
     1321 caaaaaaagt atttctaaaa taaattataa tgttcaaata aataaaatct tggtatgcta
     1381 cttgctcttt aagagaagta gcatttttta ttgttcacaa ataagttaag gggtgaagaa
     1441 attggctaaa aagaaatatc caataatagg aatatcaggt aatttactaa ttgatgaagg
     1501 aggaatgttt cctggctatg aaagggctta tgtaaataat gattatatac aatcagtagt
     1561 tatgtgtaaa gctataccat atattgtacc gattgtatat gatgatgaga ttataaagga
     1621 gcaggtatct aatatagatg cgcttatact atctggggga caagatgtaa atccactcat
     1681 atggaaagaa gaacctcata ataagttggg ggcaatatca ccaaagagag atagttttga
     1741 tatgaaatta ttaaaacatg ctttagatat gaaaaaacca gttttaggta tatgtagagg
     1801 agaacaaatt ataaatgtaa ctgaaggagg tagtttgtat caagatttat ctttaattga
     1861 aggtgcttat attaaacaca atcagcaaca tctatctaat gtacctacac atacagtaca
     1921 gataaaggag gggacaaaat tatatgaaat actaggagaa aaagaagttt tagttaatag
     1981 ttttcatcac ttagttgtca acaaagtagc accgggatat attgtttcag ctacatctaa
     2041 agacggttta attgaagcta tagaaaagga aggaagtgag tttgtaatag ggatacaatg
     2101 gcatccagaa atgatgacta gagattatga taatatgaaa aaaatattta tggcaatagt
     2161 taaagaagca tctaaataaa aaatataaat atattaagtt ggctttgggg ggaataacaa
     2221 tggaaaaagg cattaagtta ggtttttgga gtattgtact attaggaatc aatgcaataa
     2281 tagggtcagg tatattcggg ttacctggtg atgcttatac agatataggt ccagcaagta
     2341 tattagtatt agttttttgt atgcttttag ctgtttctat tgctttatgt tttgctgaag
     2401 caggtagttg gtttgatact gatggagggc cctatcttta tgcaaaagag gcatttggag
     2461 attttgttgg atttgaagtt ggatttatga agtggatagt tagtatgatt gcttgggcta
     2521 ctatggcaaa tttctttgct gtaacactat catctgtatg gccacaagta gcagaaccac
     2581 ttattaaaaa tataataatt ggtatattag ttgtagggct tgggattata aattttatgg
     2641 gaatgaaaca atctaagcac ttaaataata tcatgactat aggaaaatta ttaccaatag
     2701 ttttatttat agctgtagga ttattcttta taaagggttc taattttacc ccatttgtaa
     2761 taatacaaaa aggtcaaagt gcgtcatcag cttttgtagc agtatctata actttatttt
     2821 atgcttttac aggatttgaa tctttagctg ttgcagcaaa agatatggag aaccctaaga
     2881 aaaatgtacc taaagcattg gtaatggtaa tgtttgtagt atcagtaata tatatgctta
     2941 tactgggaat aagtataggg gtgttgggta atggcttagc agggtcagct actcctgttg
     3001 cagatgctgc cattaagatg ttaggtccta taggtgggta tataattaca ataggaacta
     3061 tagtatctgt tggaggtata aatattgcat catccatatt tacaccaagg tctgctgctg
     3121 ctttggttga acaaggatta atgcctaagt ctataagaaa aactaataaa aatggatctc
     3181 cttatatagc aattatagta tctgtcatag gaactttact aatagcatgg tcgggttcat
     3241 tcacaacatt atctcaaata agtgttgtat ctagatttat tcaatatatt cctacatgtt
     3301 tagctgtact aatattgaga aagaagtatg ctggtaaaga tgtaaatttt agaatacctg
     3361 gaggagcaat tataccaata tttgcagtta ttataagtat attattactg ataaaagcag
     3421 gtattgatga acctatgaag atagtttggg gattaggtgg gatgataatt gtagttccta
     3481 tatatttcta tatgactaaa gtatattcaa agaaatataa tgatgtagaa gtcaaataat
     3541 tattgtaatc tatagatatt ctataaatat tactttgaat ttagtaaaat tggtataaca
     3601 ccagggtgtt ataccgattt ttacataaat aactttattt ataatatatc attatacttt
     3661 cgtaaggttt tatttccatt ttattttcaa ttatatctct agtgtaattg gtttcaagta
     3721 ttgtgtagtt taatttattc aaataagttt caggtaattt atatactaca ttatcatgag
     3781 taaaattact tataacaaga agattttggt ttttatactc tctttcataa acaaatagat
     3841 ttttatgatt ttcatctaac aatctataag taccttctgt aaaaatatca tattgtttac
     3901 gatatttgat taatctttga taagtataaa atactgaacg agaatcatta agctcatttt
     3961 ctacactaat attgtcatta ttaactttta accacggttt atgattagaa aaccccttat
     4021 aaatagtatt atcccattgc ataggagtac gggcattatc tctggatttt tgttgaagta
     4081 taagcaatgc ctccttttca tcaataccag atttaatcat atgatagtat gcattcttac
     4141 tttcagcatc atcatattca tctattttag taaagtaggc atttgtcata cctatttctt
     4201 caccttgata tatgtatgta gtaccctgta gaccaaataa tgttatggct aacatcttag
     4261 cactttcttg acgaaaaaat ctatcatctc caaatctaga taaagctcta ggttggtcgt
     4321 gattagagta aaatagagca ttccatcctt ttccttcata cattttatat tgaaagtcag
     4381 attgtgcttt ttttaattta tccagagaga acatttcatt agtccatttt gcgcctcctt
     4441 tataatctgt tttcatatga tggaagctaa aaaccataga tagttcttct cgattttctc
     4501 tagtatagtt gatacactct tcaggtgtag tagatgacat ttcacctaca gtaactatat
     4561 ttttaggttt gaaaactttt tgattcatat tatgtaaata ttcgtgtata cgaggaccat
     4621 cagcgtagtg agttcttcca tctccttttg gtcctgttat agggtcatca ggtaatccag
     4681 gtgttttgga gagtaggttg actacatcta aacgaaaacc atcaatacct ttatcagccc
     4741 aaaattctag tattttgtaa cattcttcac gaaccttttc attttcccaa tttaaatcag
     4801 cttgagtttc ttcaaataaa tgtaaataat attggttagt ggtttcatca tatttccaag
     4861 cagttccact aaatctggat atccagttat taggaacaga accatcaggt ttagcatctt
     4921 tccagaaata ataatcacga tatggattgt ctttgctttt tttagattct ttaaaccatt
     4981 tatgttcagt agatgtatgg tttaggacca tatccatcat tatttttatg tctcttttat
     5041 gagcttcttt taagagtttt tcaaagtcac tcattgtccc atattttggg tctatattat
     5101 aataatcttc tatatcatat ccattatctc tttgaggaga aacgtacata ggagtcaacc
     5161 ataataaatc aacacctaaa gaatacagat aatctaattt ttctataata ccatttatat
     5221 cacctatacc atcattgttg ctatctttga aactttttgg atatatttga tagacagttg
     5281 cttttttcca ccaatttttc atacttgata cctcctgtaa tttataagtt aattataata
     5341 tgtttaaaca aattgtcaag cgttaaacta atattaaagc taaaatatac aataaataat
     5401 tatttgtata aacatataat tgtatataga atagtgaggt tagtatggga taaagtgtct
     5461 aaacgtaaaa aaattatgtt taattaaaca taattttttt aatatctttg agtaacatcc
     5521 atgaatacaa atgtttcagg tgtatgcttt gattcagtat attcaaaaag ctttccatca
     5581 tcagtatata cactattttt aataacacca acacaattat aatcatttag aggtagcgtt
     5641 tttaactcaa gctctgtagc tttttcaatt ttaaatatct ttctggatga aacaattttt
     5701 gtacctagac actcttctat atatttataa atagagcctt gtgctataga aacatcaagg
     5761 tctttaacta tactacatag aaaatagtta atgtcgagaa ttttaggtat gttatcaata
     5821 tatcttagtc tgtgaaggta atatacttca ctaccaactt taaaacctgt ctttttagat
     5881 aacttattat caattaagat tttgctgaag tggagaacac tagtagcagt attaaccttt
     5941 ttatcttcta caatcgattc tacacctttg aaattgtgca tatttagaaa aaaatcatta
     6001 gaacaactat tttctaacac tacaacaccc ttgcctttta tactttgaac atatccttct
     6061 gtgtttaatt gagaaattgc tctacgtaca gtgtttctgc tacatgaaaa cctttcaata
     6121 agttgaagtt cagtaggcag catcatatta cttgtatatt taccatcaag tatttcctct
     6181 ttcaatactt catatatctc tgaaaattta tttttagcca tataccccta tccctcctta
     6241 aaggtcttta gtaatattat tgaactgata tgaacaaatt atatcaaatg tgtttagaca
     6301 tgtatagtga aaatagaatc atatcaacca taaaaaggac aaaatagaat tattttgtgg
     6361 gttgtttcaa atacatggtt aaacatattt ttaaaataaa aaaaatgaat taaaaaaata
     6421 gactagatat agcaatacac ttaaaaggat ttatatgtta aattaaatga taaaaataaa
     6481 atgaataaaa aatattgaca atttgtttaa acaaatataa tatttaaaat ataaaatgtg
     6541 aacaaatcgt tttcacaaaa aattaaaccg aaatagaaag gggattggat atgaagaggt
     6601 tcttaaacaa aatgatgcaa caactgtcac ttttaggaaa gactatgcta gtgccaatag
     6661 ctgtaatgcc agcagcaggt atattaggat tattatttgg tccaaatatg ctcaatgtgc
     6721 cagctatatc taatatatct aatatagtat tttcaaatgt ggactactta tttttattag
     6781 gagcagctag tgcctactca aagtctaaag acaagacaag tgtaatggtt gcagccgtag
     6841 ttgcatatat gatatttaag tcaaatttag agttattgaa tgatacatta aatgcaggtg
     6901 tatttggtgg gataattgtt ggagcttgta tagcatggac ttacaataaa acatataaag
     6961 ttaaagttcc agcattttta agcttctttg caggagaaaa atgtgtaatt acactatcac
     7021 cagtggttgc tttaatttta tcatatatat ttagtttatt atggatatat ccacaagctg
     7081 caatgaatca atttggtttt tggttgggag gagctggtgc aataggaata ttcttgtttt
     7141 tattctgtaa ccgtgcacta ataccaactg ggttacatca agttttaaat gcttacatat
     7201 tatttgaaat gggtgaatat acaacatcaa caggagaagt tataagagga gaaattccta
     7261 gatttatggc aggagaccca aatgcaggat tcttctggac aggattttac ataattatga
     7321 tgttttcaat acctgctatt tcttatgcta tatataaaac agcacaagat aaaaagaagc
     7381 aagaagtaaa aggtgcaatg accgctggag cattgacttc ttttatggca acagtaactg
     7441 aaccaataga attttcgttt ttatttgcat ctccaaagtt atatataatt cattcttttt
     7501 attctggtct aggtggagtt gtactatatt tactgggtgt tcgcttgggt tcatttaatg
     7561 gagcaagtat aattgactat gtcttaagtt ttagttatgg agataaagcg tggttagtta
     7621 tacctgtagg agttgtattc tttgtattat attatgtaac atttaaatat ataataataa
     7681 aagataatgt gcaaacacca ggacgtgaag ttgagataga gataggtgat aaagttagtg
     7741 aaaaagaaaa gaatttaaaa ctttctcatg gtaatttttc atatatggct aaacagataa
     7801 ttaaaaattg tggtggttat gaaaatatag tgactcttaa taattgtatg acgagattac
     7861 gtttagaagt gaaggatgct actattttga atgatgataa tattaaaaaa acaggagcca
     7921 aaggggtaat taaattgagt aatacatcag ttcaaatcat cataggaacg gatgttgtta
     7981 aagtaaagga tgagatggaa atgcaacttg atgagttgag aaagcaggca gacgcataaa
     8041 atgaagaaac tagtagttag ggcagatgat ataggatatt cagaaggtgt tacactaggg
     8101 ataatatctt cttataagaa cggtattgtt acaagtgctg gaatcatggt gaatatgcct
     8161 ttttcaagac aagctataga aatggtaaaa aatgatgatg tatgttttgg tttacatgtt
     8221 aatttagtaa taggagaacc ttgttgcaaa gaacatatta agataaagaa tttgttagat
     8281 gaaggtggaa aatttgtttc tagcaaaata agaagaacgc aactactgac aggtaaagag
     8341 gtttttaatt atgaagaagt atatgaagaa gtttgtgctc aaattgaaac ttacaagttg
     8401 tatatgagta aattgcctga gtatattgat atacatggta tagaagaaga tgtatcaata
     8461 tcagcaatgt gtgatgctgc aaaagaatac ggtataccaa gttgtcccta ttatttggat
     8521 tcatgtatta gcatgccaag taaatatcct cagaatgatt tttacaagtt aaataaatct
     8581 tttgttaaat tatttgaaga aaaatatata aatttagata aagatataac attgatggtt
     8641 actcatccag gatttataga ttatagtctt ataaaatcat cttctcttat tcaagagaga
     8701 gtatatgaac atgcaatgct atgtgataag gaagttaaaa tgtggttaaa aagaaataat
     8761 attgaattgg ttgattttaa agatattttc aagtctaata attatatata aagaatagat
     8821 tacctaaagt tttaattaaa aataagtatt tgaaaaaaat gtgtattttc taaaaaaata
     8881 ttgatagaga tgtatcaaaa taaaaacttg aatcatctct atcaatatag gaagtacaca
     8941 ttttttatgt ataataaaaa gagtagtatg cattaataaa ttaaaaatat taaattataa
     9001 ttatattaac gaaaatttta gctaatattt ttgaatttta cttatgaata ataaaataga
     9061 tggaggagaa actatggaat tttatgaaag agcaaaaaaa tatgagtata aatttacaga
     9121 tacagaagac atgataattg aatatatgag aaaaaacaaa gaagaaatag taaaattatc
     9181 aatacaatct ttagcagata aattttttac tgtcccaaat accatagtta ggctatgtaa
     9241 aaaaatggag tatgatggtt tctctcaact aaaaaataat ttaaaagaag agttaaaaaa
     9301 tgaggataaa gagccaaaaa atggttttga atcagatata aaaaagacat tagagctaat
     9361 agattatgaa aaaataaata aagtagcaga tataattcaa aaatctagga gtattatagt
     9421 ttatggaata ggtcaaaaca ttcctttatg tgagatatta gtaaaagaaa ttagaggtgg
     9481 gcatgataat ataacttact atagtcaaag acatgaagta cttcatgata tagggaaatt
     9541 aggtgaacaa gatatgtttt ttgcacttag tttaagtgga gaaacgaaag aagttataga
     9601 agcagcaaag acagcagtta ataagggttg taaggtagta gctctaacac atatatgtga
     9661 aaatcaattg caacaaattg cagatataag tttatattgc tattcaacaa ttaaacaagt
     9721 aaatgggtac aatatatcag ataaggttcc aattatgatg attatgagag caataataga
     9781 gcaatactgg ttaagataga gatttgtgta taaatacaca aatctttatt ttttaagaag
     9841 aaattgtgtg tataatttca aaaaaacatt ttcataattt attttatgct atattcatag
     9901 cattaaatga tttaaggagg gcgtaatttt ggaatttaag aaaatcacaa atgaaaacct
     9961 tgttttttta aaacaaaaaa taaaaacaaa ggaagacgca ataaagttct tagcaagaaa
    10021 gctatattat gaaggcaagt taacatctta tgaggaatat ttagaagctg taatgtacag
    10081 agaaacttta tctccaacag gttttgaaag agggcttgca attcctcatg gaaaatctaa
    10141 gtcagttaaa gaagcttcat ttgcagctat gaccttagag acaccaatag aaaattggga
    10201 aagcgtagac ccaaataata aagtagagct aatattttta atagcagtac cagaaaatga
    10261 aggtggaaat acacacatac aaatactttc tgagttagtt actagactgt caaatgaaga
    10321 ttatatgaat atgttgttaa attcaaaaac taagaaagag ttatttaata acttagatat
    10381 aaaagaaaag aaagaacaaa taaaagaaga gattaggatt caaaatgaat caaaaaaaat
    10441 tatattagca ataacagcat gccctgcagg tatagcacat acatatatgt cagcagaagc
    10501 attaattaaa gctggtaaag aaatcggtgt ggatgtgtat gtagaaaagc aaggagctaa
    10561 tggtatggtg gaccctcata caccagatat aataaaaagg gcagatgcaa taatatatgc
    10621 aacagatgta gcacctaaaa atacagaaag atttgaacat ttgccaaaca taaaaacgtc
    10681 agtagcagct ccactaaaaa gagcaaaaga aataatatac gaagctttag aagtagcaca
    10741 aaaacaaggt aaaggtgatt atatagaaaa atcatctgat atatcatatt gtgaaaaatc
    10801 atcatggaaa aaagatgtaa aatcagcaat acttacagga gtatctcata taataccact
    10861 tattgtggca ggaggtatga tacttgcatt tgcagtatta gcatctcaag catttggtct
    10921 acaagaacta tacaaccaag aaggttcttg gttatggcaa ttaagaaagc tttctggagg
    10981 tatgttaggt acattgatgg ttccagtatt atcagcatat atgtcttact cactagctga
    11041 aaaaccaggt cttggaccag gatttgcagc aggtctagca gctgatttaa taggtagtgg
    11101 atttcttgga ggtatgttag gaggtttact tactggctat atagtaaaat ttttaaagaa
    11161 gaatatacca gccaaaggca cattagctgg attcgtaagt ttttgggtat atccagtagt
    11221 tggttctttg gtagtaggtt tattgatgtt atttgtagta ggaaagcctg tagcagcact
    11281 taacaatggt cttataaatt ggttaaatgg gatgactgga gtaaatgcaa tactactagg
    11341 tgcaataata ggagctatgg tttcatttga cttaggtgga ccaataaata aagcagccta
    11401 tacatttgca ataggagcaa tagcaagtgg aaattttata ccttatgcaa tatttgcatc
    11461 agttaagatg gtttcagctt ttggagttac aatagctact gttttaggca gagatttatt
    11521 ttcagaatca gaaaaggaaa taggaaagca aacttggata ttaggattgg ctggaataac
    11581 tgaaggtgca ataccattta tgatagagga cccaatccgt gttataccat cattggtagc
    11641 tggttcagcc ataactgggt ctatagtagc attcttcaat ataggtcttc aagtgcctgg
    11701 agcggggata ttctcattgg cgcttttaga gggaagtatg tcaggattta ttatagcatc
    11761 aatatggttc tttgcggcag tacttggagc tataatatca gcaatattat taataataac
    11821 taggaaacaa aaaaatcaaa gaaagataca ttaaaagata cgttattaga tatttaaatc
    11881 aaaggtttaa ttatagtgat aaaataaata ttaaaaaatt ttgtaaactc agtagtattt
    11941 ttattggatt tttaattaga cagaaaatat tatcagataa gttttggagg ataagcaatg
    12001 aaaaaaaatg tacacatagt tcctcatatg cattgggata gagaatggta cttctcaact
    12061 gaggaatcta gaatattact tgtaaataat atggaagaaa taatggatat gttagaaaat
    12121 aatcataact atccatatta tgtgatggat ggtcaaactg ctatattaga agattattta
    12181 gcagttaagc ctgaatgtaa ggagagaata aaaaaattag ttcaagaagg aaggctaata
    12241 ataggtcctt ggtatactca aactgatgaa atggtagttg gtggagaatc catacttaga
    12301 aatttactat atggaataaa agattgtgat gaatttggag aatatatgaa aataggatat
    12361 ttaccagatt catttggtca aagtgctcaa atgccacaaa tactaaatgg gtttgatata
    12421 aaatactcaa tgttttggag aggatgttct gaaagaaaag gaactaataa aacagagttt
    12481 aattggaaga gtgatgatgg gtcttctgtt ttggttcaaa tacttccatt aggttatgca
    12541 ataggaaaat atctaccaat gaatgaagat gagctaagaa caagaatgga taaatattta
    12601 ccggttcttg ataagggtgc aactactgac catataatac ttccaaatgg acatgaccaa
    12661 atgccaattc aaaagaatat atttgaagtt atatataaat taaaagaatg ttatccagag
    12721 agaaaatttt tcttaagtag atatgaaaac atatttaaag agctagaaaa aaatgaggat
    12781 atagatacaa taaaagggga atttttagat gggaaatata tgagggttca tagaagtata
    12841 ttctcaacaa gaatggatat aaaatcagca aatgctagaa tagaatcaaa gataactaac
    12901 atattggagc cactagcttc tattgcatat tctctaggat ttgaatatca tcatggatta
    12961 atagagttaa tttggaaaga aataatgaaa aatcatgctc atgacagcat tggatgttgt
    13021 tgttcagata aagttcacca ggaaataatg aatagatttt tcttagctga ggaaaaagta
    13081 gaccaattaa taacatttta caaaagaaga atagttgatg caatgagttg tgaaatggct
    13141 ttagataagt tagtagcatt taacttaatg ccttacgata ggaatgaaat agtgagcacc
    13201 caagttataa ctaaaatgaa agcatttgaa atatttgata aagataataa taagttggat
    13261 tttgaagtgg tacataaaga agtaatagat gcaggattaa tagaccgtca aattgttcat
    13321 tatggaaact atgattcatt tatggtatat acaataaact ttaaagataa aatacctgca
    13381 atggggtata aggcgtatat gataaaagaa gttgaacata tgttagaaaa agcttatgaa
    13441 gtatgtgaca tggtagataa tgacttttat actattcaag ttaatgaaaa cgggacatta
    13501 aagatatttg ataaaaaatt aaataaaact tttgaaaatg tattattgat ggaaaatgga
    13561 ggagatgaag gagatgagta tgatttttca ccacttccaa atgagaaatt aatatttaac
    13621 gataatatag tagcaaattc cactataaag aaaaatagat ttaataatga gataaagata
    13681 aattatagat taaaagtacc aaaagatata caagctagaa aaaataatag agcagattca
    13741 tttattgact ttgatatagt tataaatgtt ccaaatgata aagctataat agatgtagta
    13801 ttcaacataa ataaccaagc aagagattgt agggttagaa cttatatacc aacaaatata
    13861 gcttcaaagt tttctgtgtc tgataatcag tttggacata taaagagaag tgtgtatgat
    13921 gatgctatgg aagtttggca acaggaagaa tggagtgaaa gaccagattc tatatatcca
    13981 atgctaacat tcgttggatt gtctgacaaa gagcatggag tagctgttct tacaaacagt
    14041 acaagagagt ttgaaatagt aggggaaaaa tttgacacaa tagctataac attatttaga
    14101 agtgtaggat tcctaggtaa agaagaaatg gcaagacgcc caggtcgtcc atcaggaata
    14161 aagttgccta ctccagattc tcaaatgata ggagatataa aaatagactt agcaatagtt
    14221 actcatgaaa aatctacact agaagcaaat gttgcaaata tggctaaaga gtatttaaca
    14281 aaaatacaaa cttataataa aatgccatat aatgctatga aattgaatca atcagaaata
    14341 gaagtagatt attcatatag tttacttaag gaaactaata aggaccttgt gttaagtgtt
    14401 gtaaaaaagg ctgaaaaaga agaggcatta gtagttagat tttataattc taaagaagaa
    14461 acaaaatcag ctaacatagt atttaacaaa aatattaaaa cagctaaatg gattaaccta
    14521 aatgaaaaag aattagaatc tatatcaata gataataact ctgtaaatgt agagtgctta
    14581 atgaaccaag ttaaaactat actggttaaa tagtgctaaa aatgattaga tagtgctaaa
    14641 aaggaattat cttaaaatag agataatcct ccttatcatt gttgaaataa taaacatata
    14701 tttgatttta tacaaaagag tgtctcatga actaagaaat ttattttgag acactccctt
    14761 tttataagat aaaagaatat taatggtata agttgtatac aaagagagat gtagagtata
    14821 aaataaaaga taaattaagc ttataaaatt atatgtttaa aataaggagt aataaaaagg
    14881 agtagataag tatgaaaata ttaatttcta tagattcttt gaaaggaagc ttatcttcaa
    14941 tagaggcagg aaatgcaata aaaaagggga tattgaaggt taaagaagat gcgcaagtaa
    15001 agatattgcc acttgctgat ggtggtgaag ggacagtaga tgctttagtt caagggatga
    15061 atggtaaaaa agagactata gaagttactg gaccaatagc taaaaaagtg gatgcaacat
    15121 atggattatt aaaaaataca agtacagcaa taatagaaat agctcaagca tcaggattaa
    15181 cattggttcc gactgaactt agaaatcctt tatacactac aacttatggt gttggggaaa
    15241 taataaagga agcaataaat aaaggctata gaaattttat agttgggata ggtggaagtg
    15301 ctactaatga tgctgggata ggtatgcttc aagccttagg gtttgagttt tatgataaaa
    15361 ataacaagtt agtaggatta ggtggaaaag ttttaaatga aataagacat ataaaaatag
    15421 aaaatagatt aaaggaatta gatgaatgta agtttaaaat agcctgtgat gtaaataatc
    15481 cgctgtttgg gaaaaatgga gctgcataca tatacgggtc tcaaaaaggt gcaacatcag
    15541 aaataattga ggagttagat aatgggctta gaaatttttc caaagtagtt aaaaattact
    15601 taagtaaaga tgtagctaat gtagaaggag caggagcagc aggaggatta ggttttgctt
    15661 ttttagcatt tttaaattca aaattagaat ctggaataaa aataatatta gaagaaatta
    15721 aattagagga agagttaaaa gatgcagatt ttgtcataac tggtgaaggt agattggaca
    15781 atcaaactgc tatgggcaag gctccaatag gagttgcaaa actggcaaaa aaatatgagg
    15841 taaaagtaat aggtttggct ggagccacaa cagaagatgc agttaaatgt aatgaagagg
    15901 gtatagatgc ttatttttct atagtaaata gagctatgac aatagaagaa gcaatggata
    15961 aagcaactgc tagtgagaat atgacagcta caacaacaca aatattcaat cttataacta
    16021 gtatacaaca gtcaaaaaat atgtagtatt aaaacaaggt atattaggta aaaagctgtc
    16081 acatattaat ttagactttt tagtgtgaca gcttatctat tttatgttaa taacttaaga
    16141 aacatatatt ggaaataaat taattgattt agatattttg aataagataa gctattctat
    16201 tcataaaact actatagtta tctgccttag taagttcttt aacattattc cactcagata
    16261 atatttcaat aaagctttca aatatgtaat tgaatttttc tttttctttt tcatttatag
    16321 gtatcatagc tataatctga actttttcct caccccattt aactggttta tcgttaacaa
    16381 ttaaacatac tccagttttt aaagcatcca ttttcatgct atgaggaatt gctacattgt
    16441 tattaaatgc agttgaagac attttttctc ttataagaac atcatctata taattgggtt
    16501 tgacatattt attttcgtac agaatatttc ccataaattt tatataatct ttagcactat
    16561 ctaaatatat atttttcata aacaaagatt cactaaagta catttctaaa tgattttgta
    16621 cagtaagtat attttttcta tctttaattt gagtaaattt actttgaata ttatcataat
    16681 cctttctatt taggtaagga tttacataaa caaaaggtat agggcatcta ttgttaattg
    16741 gtaaagtagt aataaccatg tctaccttat tgtcaaaatc aagattatga gtatctgaaa
    16801 aaatctgaat aatttctatg gattcattaa atctcttctc gaggtctctt actattttga
    16861 attgtaaatc atagtaattt ggagtaatta atgcacatag aactttatct tctaataatt
    16921 tttgtctatc aaagaatgaa cctacatgaa gagatatata agatatttca tcttcgtcta
    16981 tatctacatt ttctaaagtt tgaatctgat tgcaaatata aacagatata tcatatatca
    17041 aagggtagga atcttttaac ttttgaggta tttgatttct taaaacttga ttatttttgg
    17101 ctctactgat taaatttttt acatgaagag taaattttac aacaaattcc tcatcagata
    17161 aatctataag atataagtca tatacatttt taattatttt tttagttaat ttaacaaaat
    17221 gttcatctat ataatttggc aatgacttag tatccatttc atgataattt aagactgtgg
    17281 ttttgtttgt taactgaaat actaagtagt ataattctgc actagttaaa gttatattgt
    17341 attcagactc taaaaaattt gatatatctc ttgaaatact tatatcatta ttattatcta
    17401 tgtagtttac tacttcgaca gattctatgt agttattctt ttttattctg tctatagtta
    17461 tcattaaatg aagaag
//
LOCUS       DAELLV010000059        17365 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.059, whole genome shotgun sequence.
ACCESSION   DAELLV010000059 DAELLV010000000
VERSION     DAELLV010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17365
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.059"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            76..2139
                     /locus_tag="KPD58_001612"
     CDS             76..2139
                     /locus_tag="KPD58_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0265389.1"
                     /translation="MTKIILSNYRCFGEEPTTIEINNLTGFIGHNSSGKTALLSALMK
                     LFGEKSSDRIIERSDFHIPESIDPDSISKNELYIEAQFEFPELERGKDSISIPVFFKN
                     MVVSEPGGKPYLRIRLEANWEKSSNPEGSIDSNVYYITSTTEEINPEDKRNIKRYELD
                     QIKMIYVPAIRRPDEQLKNVSGTIISRLLNGISWSEQTKARIKTKASETEEIFFEEKG
                     VESLKKSLETQWNSYHKDIRYSNPKINFNSTDLETILKKVEVTFSPTGTHRDYRVEEL
                     GDGLRSLFYLSLVNTFLEIEEEILKSINDGKEEDIFTLIPPVLTIVAVEEPENHISPH
                     LLGRVVEKLNFISTKENSQTILTSHSPAIVKRIEPTDIRYFRICKEYECTKVKSIILP
                     NEEKEAEQFKFVKESVLAYPELYFAKLVVLGEGDSEEIIIKQMIELKAESVDSSEISI
                     VPLGGRHVNHFWRLLNDLEIPFITLLDLDRERDGGGWGRIKYAIQQLINIGHDKNVLL
                     YTCEGISQDNEFKEMHTWDVTDTENMAKWIEMLKGYDVYFSAPLDIDFAMLQSFKDKY
                     ISALTSAEGPRIKDHGKIQDIVINEDAEDESLKAAYKDRIDSDIKSTLKQEGGDGSTY
                     TEGEKELMIWYSYFFLGRGKPTTHLSLFSNNTDIDFESFPYCLKEFVNSIIEKMIDDS
                     GGGRK"
     gene            2136..4001
                     /locus_tag="KPD58_001613"
     CDS             2136..4001
                     /locus_tag="KPD58_001613"
                     /inference="COORDINATES: protein
                     motif:HMM:NF024642.1,HMM:NF024754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBG0265390.1"
                     /translation="MKRNWVSKEDWSPAAGFRLEVEAMNTVINDKNTLVVAGPGAGKT
                     ELLAQRACFLLETNTCRYPKKILAISFKKDAAENLKERLELRCGKELASRFESKTYDA
                     FAKEIVDRFRNSLDEAYRPLNNYNIAENRDVRRAFEIAGLNFRGNQTEFNKVYGNKLS
                     INKLPLHTDFVDKIFIDAWNVLLKGNKDTNFVSSLTFGMISRLAEYLIRTNRYIKKAI
                     EMTYSHVFLDEFQDTKSIQYDLVKACFFKTETIITAVGDGKQRIMLWAGARKSIFEDF
                     QNDFLSQKRTLIMNHRSAPRLVEIQKAMYNSLNEEAIDLKTNEKWNYEDGEVYLRLFE
                     DYIKEADILSEEIVELVETGIEINEICILVKQSVDIYSKEIINKLSERNIKARNEAMY
                     QEILKEDIIKTLVSLFRLSLSDRNPDDWDYIYDILGELYGIDEGYNPKILNDFITKVE
                     LMINDLKVNLNITDEKQFSDLVDSKVEYISYEKFASKFQKYKSKDYFDDLVDKFKKLV
                     WIEYNETKDWIKAIDNFEGKNSIPIMTIHKSKGLEYDTIFFVGLEDSAFWNFKNQPDE
                     DRCAFFVALSRAKRRIDFTFSLDRPAGFSNSQKHYCINEFYELFDNTEIVKKIEY"
     gene            4125..4939
                     /locus_tag="KPD58_001614"
                     /pseudo
     CDS             4125..4939
                     /locus_tag="KPD58_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728762.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4964..5452
                     /locus_tag="KPD58_001615"
     CDS             4964..5452
                     /locus_tag="KPD58_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBG0265391.1"
                     /translation="MYEKDYKQYIKLDIPIDEAKIKNRFEIRLKNERAYHAAVDLLAN
                     RDEEKTAFSIINRYIRFVDKDDEKIRSEWKTNDQWSYFIGEGRAKLKLTTNPEPYNIQ
                     KTLNWLSRQVAPTLKTIKRLDSLNNSTLIEGLIRYARLTDKHKKIIEQNSVNVEDIIY
                     DE"
     gene            5565..5726
                     /locus_tag="KPD58_001616"
     CDS             5565..5726
                     /locus_tag="KPD58_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265392.1"
                     /translation="MAIAIIGVYLGFKREFSKLIGFLVVALIAVGLVFNTAGVKDILL
                     ELFNKIIGV"
     gene            5966..6193
                     /locus_tag="KPD58_001617"
     CDS             5966..6193
                     /locus_tag="KPD58_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0265393.1"
                     /translation="MDIVKVFANNVRKYRNKKGLSQEAFAEKAGLHRTYICAIERKKR
                     SIALDNVQKIADALEIETYLLFIEGEEKECL"
     gene            6190..9129
                     /locus_tag="KPD58_001618"
     CDS             6190..9129
                     /locus_tag="KPD58_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HBG0265394.1"
                     /translation="MKKLPIEKLIENLGFSGSKNLVNIDMLTKLDISKHDVKTLKVIN
                     PYAIYLVNSKPFILFIESSFEEKKLQQISKLIWNAQIPIAFICDSNSVKIFNGKALDL
                     KSYLIEEIYNQSIEDFSEESEFSYLKISDPLFWDQYTKQYTKTHLNDYLLDNITSLTN
                     KLKKKYKVKFATKLVLRLIFIRYLIDRGVDIAYKNFNHDIKASQKELLKITKNKVELY
                     ELFKYLKEKFNGNLFELNDEINNTCLTDEVFILLSDFLSGKISIDNGQMSLFSMYDFN
                     IIPVELISNIYEILLGKEIRNKDNAFYTPNYLAEYILDKTTLEFLKNNRQYKILDPSC
                     GSGVFLVNSFRRIIDMNLGDKLFCEDDKLLKELLLSNIYGIDINEDAIDVTIFSLYLT
                     ILDYKDPKILSEFKLPNLKGCNLIASDFFDDNKLESLKKINFDFIIGNPPWGSVSDGL
                     HLDYCNKNGYNDMQQNKEISRSFIFRAKDFSSRNTVCSFVVHSKILYTQKKPSKKFRE
                     FLLKKSQIFSVVEMSSVRKLVFKNADAPAAVISFKYNESEENLNNRIIYTSLKPNVFF
                     KLFNIIVIEKYDVKYISQKLLLENDWAWKTIVYGFSKDIDIIMNIKKKYQTVNEIILK
                     NSFVHGTGIQINGNESHDATHLKGKWYVDSKKGIDSFYVDINKGKELTQDKMRRPKAN
                     QQEIFIPPYVLLKKGVDTKTYRYRAAFSNDAFVYSDAVTGICGKKDNLTTLYCLTGML
                     NSSFYSYLNIMLGTSIGIEREQGFSTEIFTYPAIESDEIANLVVKIQEATKLEKTTMF
                     LNEKNSCKYIKQLDKLILKSFNLNDDKFIDYAINIQIPELTNSKSTNIYRKITKEDLI
                     NYSECFKEQFSSIYRRVGKSISISVYPKILNRFTVFELEVTDEKTDREIMVKAEADNN
                     MELFTKIIRYDYNDVFHQIRDIIHFGENSFYIIKPNCYKYWHPAIAEMDLADVLDQIL
                     SDTGGEE"
     gene            9129..9803
                     /locus_tag="KPD58_001619"
     CDS             9129..9803
                     /locus_tag="KPD58_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265395.1"
                     /translation="MGGFKNSSLNFYLKQGLYEIIIDGLIDCCLLMSKSCIEQSRKIL
                     NHEEKIRTHLVENYLENDIERRKTSLSKQHLRFEVEAQENYDNKNESYEGRVDIKVIS
                     KDTLADRRAYYIIECKRIDGKETLNKKYITEGVQRFITEKYSSHYGKNIMLGFIVKDI
                     NIQSNIQKINSLNQNILYTYIDTDMLTVRSDSENNYAVCESKYKVKSKLLLLNHIFYN
                     FSSIIR"
     gene            9964..10380
                     /locus_tag="KPD58_001620"
                     /pseudo
     CDS             9964..10380
                     /locus_tag="KPD58_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455547.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            10340..12787
                     /locus_tag="KPD58_001621"
                     /pseudo
     CDS             10340..12787
                     /locus_tag="KPD58_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005423199.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            12927..13349
                     /locus_tag="KPD58_001622"
     CDS             12927..13349
                     /locus_tag="KPD58_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265396.1"
                     /translation="MRLEDIIDKESYEEKYDDLTVEIKKLSKEKKTLKASLINEKSIK
                     ARLREFRKILERNELLEKFDRYVFASIVDKVIVGVINEDGIVDPYKITFVYKTSFDDK
                     QDGHKFINKSPLETYSKDNNDNKKKCSYTNNETLRDCS"
     gene            13887..14921
                     /locus_tag="KPD58_001623"
     CDS             13887..14921
                     /locus_tag="KPD58_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH domain-containing protein"
                     /protein_id="HBG0265397.1"
                     /translation="MFKKKLIAVAMSITMISVGSFSYAHSGRTDSSGGHRDNKNKSGL
                     GSYHYHCGGYPAHLHNNGGCPYTGGGSSSGTTTSVNNEEKQKRSVGEKGYNQGYEDGY
                     KGNYSSSNYSGDYSDTYESKYSEGYEKGKAKLEEEEKVAKETGYNLGLTGAKSNNTYE
                     KEVLKNAYDTGYSTGYNEYKTKKIEEYKAKGIEDSNKDKEKMTFEENIDSEFIDAYNN
                     AYDERQEQLKNDYTTQGFESAIKGERFDTSTIGNVKYANWFKEGYDNGKVKLPKAKES
                     VYNQGYNEEDFSIPDEMKSIETKLKGVYDEGLEKREEEKSRNATYGVGAGTAAVVAGV
                     VYKKKKRKKI"
     gene            15008..15211
                     /locus_tag="KPD58_001624"
     CDS             15008..15211
                     /locus_tag="KPD58_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HBG0265398.1"
                     /translation="MGFNFRKSINLGGGLKLNLGKKSVGISAGVKGARVSVNSKGRKS
                     ATLSIPGTGISYTKTSTKKRKPK"
     gene            15382..16542
                     /locus_tag="KPD58_001625"
     CDS             15382..16542
                     /locus_tag="KPD58_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp27"
                     /protein_id="HBG0265399.1"
                     /translation="MKLSKILLAIGMSVSLLIASCPTSNALSSINSIKGTNKYETAGL
                     IADKQNYDIAILINSENGLSDGLSASGLSGAKNAPILLTKKDTIPSETLKRLEKVKEV
                     YLIGGKNSIGASVQKILSDKKISINRISGTDRIDTSHKVAKEIGRVSTVKKVILTNAF
                     KGEPDAMSVASIAVRDKSPIVLTDGNTTSFATDGLETYAIGGKVSMSDKLINETKATR
                     IGGVDRYDTNKQIINKFYNGSKEFYIASGTDLVYSLVGSTIAQKTPIVLVGNGSNKSI
                     LKNASKITAIGNIDDVVIKQCLNITNNVGDSNTGIVKDTNSNNTNKPSNEPSSNNPGL
                     TDKSTVYVTPNGKSYHLTKNCSTLKRSKTINKVTLKQAKSQGKIDPCNVCVK"
     gene            16801..16941
                     /locus_tag="KPD58_001626"
     CDS             16801..16941
                     /locus_tag="KPD58_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265400.1"
                     /translation="MENLMMSIITGVIASYIYDKLKSHSERPTKSGWELNIKFQKSKS
                     SK"
BASE COUNT         7115 a         1682 c         3006 g         5562 t
ORIGIN      
        1 taataaagga aaaaatacag aattaaaaaa cataagatag ataagtggac ttaggggaga
       61 gaaattatta tgcaattgac aaaaataatt cttagtaact ataggtgttt tggagaagaa
      121 cctacaacta ttgaaattaa taatttaact ggatttatag gtcataatag cagtggtaaa
      181 actgctttat taagtgcact tatgaaactt tttggagaaa agtctagtga tagaattatt
      241 gaaagatcag atttccatat acctgaaagt attgatccag atagtatttc taagaatgaa
      301 ttatatattg aagcacaatt tgagtttcct gaattggaga gaggtaaaga cagcatatct
      361 ataccagtat tttttaaaaa tatggttgtt agtgaaccag gtgggaaacc ttatttaaga
      421 attagacttg aagcaaattg ggagaaaagc tcaaatcctg aaggaagtat tgattcaaat
      481 gtatactata taacatctac aacagaggag ataaatcctg aagataagcg taatatcaaa
      541 agatatgagc ttgatcaaat aaagatgatt tatgtacctg ccattaggag accagatgaa
      601 cagttaaaaa atgtctcagg aacaataatt tcaagattat taaatggtat aagttggtca
      661 gaacaaacaa aggcaagaat taaaacgaaa gcaagtgaaa cggaggaaat tttctttgaa
      721 gaaaaagggg ttgaatcatt aaagaaatca ctcgaaactc aatggaatag ttatcataag
      781 gatattagat attctaatcc aaagataaac ttcaatagta ctgatttaga gacaatttta
      841 aaaaaagttg aagtaacttt ttcacctaca gggacacatc gagattatcg ggttgaagag
      901 cttggagatg ggttacgttc tttattttat ctttcacttg taaatacatt tttagaaatt
      961 gaagaagaaa ttctaaaatc tattaatgat ggtaaagaag aagatatatt tacattaata
     1021 ccgcctgtat taacaattgt tgcggttgaa gaaccagaaa atcatatttc acctcatctt
     1081 ttaggaaggg tagttgaaaa attaaatttt atttctacaa aagaaaattc acagacaata
     1141 ttgacatcac attcaccagc aatagtaaaa agaattgaac caacagacat aaggtatttt
     1201 aggatttgta aggaatatga atgtacaaaa gtgaagtcaa ttatattacc aaatgaagaa
     1261 aaggaagcag aacaatttaa atttgtaaag gaatcagtat tagcatatcc tgaactttat
     1321 tttgcaaaac ttgttgtatt gggcgaaggt gatagtgaag agatcattat taagcagatg
     1381 attgaattaa aagcagaatc tgtagattca tcagaaattt caatagtacc attaggtggt
     1441 aggcatgtta atcatttttg gcgtttatta aatgatttag aaattccatt tattactttg
     1501 ttagatttag atagagaacg agatggaggt ggttggggac gtataaagta tgcaatacaa
     1561 caattaataa atattggtca tgataagaat gtattacttt atacatgtga aggaatttca
     1621 caagataatg aatttaaaga gatgcatact tgggatgtaa ctgatactga aaatatggct
     1681 aaatggattg aaatgctaaa aggttatgat gtttattttt cagctccgtt agatatagat
     1741 tttgcaatgt tacaaagttt taaagataaa tatatttcag cattaacaag tgcggaaggt
     1801 cctaggataa aagatcatgg aaaaatacaa gatattgtga tcaatgaaga tgctgaagat
     1861 gaatcattga aagctgctta taaagataga atagatagtg atattaaatc aaccttaaaa
     1921 caagaaggtg gtgatggatc tacttatact gagggagaga aagaactaat gatatggtat
     1981 tcatatttct ttttaggaag aggtaaacca actactcatt tatctttatt ttctaataat
     2041 actgatatag attttgaaag ttttccatac tgtctaaagg agtttgtaaa tagtattatt
     2101 gaaaagatga tagatgattc agggggtgga agaaaatgaa gaggaattgg gttagtaaag
     2161 aagattggag tccagcggct ggattcagac tagaagttga agcgatgaat acagttatta
     2221 atgataagaa tacacttgtt gtagctggtc ctggcgctgg aaaaacagag ttattagctc
     2281 agagagcctg ctttttacta gagactaata cctgtagata tcctaaaaaa atccttgcta
     2341 ttagttttaa aaaggatgct gctgaaaatt taaaagagcg tttagaattg agatgtggga
     2401 aagaattagc ttcaagattt gaatcaaaga cctatgatgc ttttgcaaaa gaaatcgtag
     2461 atagatttag aaatagttta gatgaagcat atagaccttt aaataattac aatatagctg
     2521 aaaatcgtga tgttagaaga gcatttgaga tagcaggatt aaatttcaga ggaaatcaaa
     2581 ctgaatttaa taaagtatat ggaaataaac tatcgataaa taaattgcct ttacatacag
     2641 attttgtaga taagattttt atagatgctt ggaatgtact acttaaaggc aacaaagata
     2701 ctaattttgt aagttcactt acttttggta tgatatcacg tttagccgaa tatttgataa
     2761 gaaccaatag gtatattaag aaagcaattg aaatgactta tagtcatgtg tttttagatg
     2821 agtttcaaga tactaaatct attcaatatg atcttgttaa agcttgtttt tttaaaacag
     2881 aaacaataat tactgctgta ggggatggca aacaacgaat tatgttatgg gctggtgcta
     2941 gaaagtcaat atttgaagat tttcaaaatg attttttaag tcaaaaaaga acattaatta
     3001 tgaatcatcg ttcagcgcca agacttgtag agattcaaaa agcaatgtat aacagtttga
     3061 atgaagaggc aattgattta aaaactaatg aaaagtggaa ttatgaagat ggagaagtgt
     3121 atttaagact ttttgaggat tatataaaag aagctgatat acttagtgag gaaatagtcg
     3181 aattggtgga aacaggtata gaaattaatg aaatatgtat attagtaaaa cagagcgtag
     3241 atatttattc aaaagagatt ataaataaac tttctgaacg taatattaag gcgcgtaatg
     3301 aagcaatgta tcaggagata ttaaaagaag atataataaa gacactggtt tcgcttttta
     3361 ggttgtcgtt aagtgataga aatcccgatg attgggatta tatttacgat attctaggtg
     3421 aattatatgg aatagacgaa gggtataacc ctaaaatatt aaatgatttc attaccaaag
     3481 tagaattaat gattaatgat ctcaaggtaa atttaaatat tactgatgaa aaacaatttt
     3541 cagatttagt tgattctaag gttgaatata tttcatatga aaaatttgca tcaaagtttc
     3601 aaaaatataa atctaaagat tattttgatg atttggtaga taaatttaaa aagctggtat
     3661 ggattgaata taatgaaact aaagattgga ttaaagcgat tgataacttt gaaggtaaaa
     3721 attcaattcc aataatgaca attcataaaa gtaaaggact tgaatatgat actatctttt
     3781 ttgttgggtt agaagattca gcattttgga attttaaaaa tcaaccagat gaagatagat
     3841 gtgcattttt tgttgcatta tcaagagcta aaagaagaat tgattttaca tttagcttag
     3901 atagaccagc aggattttcg aattcacaga agcattattg tattaatgaa ttctacgaat
     3961 tgtttgataa tactgagatt gtaaaaaaga tagagtattg attaagatga taaaaacttt
     4021 agtatattat attttaagat attagttata taagtagaca ttgaatttat gtatttgatg
     4081 tctatttttt atataaaaat atattaaaga aaggatgaat ttgcatgaag aaaaaaacaa
     4141 aaatatctaa aaagattgta agttttatta tggtttttat aactatagtt actactatac
     4201 ttacatcatc agtttcagct tatgctagcg agttggatat gagtgaacag acaggcttta
     4261 attatttagg tattagtcca attacaggaa aaaaattaat cataacattt atcgtatgaa
     4321 gatggataat aaaattgtat tttgtgtaga gccaggaatt aaaacttcaa gtggaggagg
     4381 atatatatcg gaggagttag gagataaatt tatatctaca acacttccaa attatcataa
     4441 aagaaaagct gaaatacttg agaaggtaaa tagacataat atattacctt catggaataa
     4501 tgaaaaagtt gcagttaaaa gtggagaaac aattgagtta gaagataaga atggtattat
     4561 tagagaaatg caattagaat caaataatac tggaacagat atattacttg aagaaaatac
     4621 tttgaaaatt acagcaaata aaaattctaa ttcaggagtt attagttata taaagattcc
     4681 taaaaatgag gttggaatat caatagttta tcgtaagcat aactatcaaa gcttatcaga
     4741 atttcatatg gaaagttcga tgagtgcaaa tttaaatata aatgtaatca agttaggaat
     4801 tgttaaagta aaaaagattg atgaaggcac aggaaaggaa ttagcaaatg caaaacttag
     4861 atttgagtat gatggtgata gtaaagaaat tataacgggt tctaatgaaa ttgctagtat
     4921 agaagatatt attggttgat taaaaagtga agtatacttt tgtatctatg aaaaagacta
     4981 taagcaatat ataaaattag atatacctat tgatgaagca aaaataaaaa atagatttga
     5041 aataaggtta aaaaatgaaa gagcatatca tgcagcagtt gatttgttag caaatagaga
     5101 tgaagaaaag acagcatttt caattattaa tcgttatata cgttttgttg ataaagatga
     5161 tgaaaaaatt agaagtgaat ggaaaactaa tgatcagtgg agttatttta ttggtgaagg
     5221 tagagcgaag ttaaaactaa caacaaatcc agaaccatat aatattcaaa aaactttaaa
     5281 ttggttatca agacaagttg caccaacgtt aaaaacaatt aaaaggttag atagtttgaa
     5341 caactcaaca ttaattgagg gcttaataag gtatgcaaga ctaacagata aacataaaaa
     5401 aataattgaa caaaattcag taaatgttga agacattatt tatgatgaat aaaaaaatgt
     5461 aattagtatt gcagatttta atataaaaga agggagatat acgaatgaat tttggtcaaa
     5521 atttatataa ttagttttta tcaaatgcac aatcattggt attaatggct atagctataa
     5581 ttggtgtata tttaggattt aagagagagt tttcaaaact tataggattt ttagtagttg
     5641 cattaattgc agtagggctt gtgtttaata cagcaggagt taaagatata ttattggagc
     5701 tatttaataa aataataggt gtgtaaggat taaaagagaa tagaaaatta tgagtatatg
     5761 atgaagggtt ctaataagtt ttattaaaat atatattgaa agacaagtag agctatttcg
     5821 agttattaca tgtaatgcta atatatataa ctgcattttg gaaattaaat acataattag
     5881 aaatagcaaa ttttataggt gacttattaa tatgtttact tattgtatac tatttgatat
     5941 atttaatatg aggtgattct tgaaaatgga catagtgaaa gtttttgcta acaatgtgag
     6001 aaaatataga aataaaaaag gtctatcaca agaagctttt gctgaaaaag ctggattgca
     6061 tagaacttat atttgtgcta ttgaaagaaa aaagagaagt attgctttag ataatgtgca
     6121 gaaaattgct gatgcgcttg agattgagac ttacttacta tttatagaag gtgaggagaa
     6181 agaatgtcta tgaaaaaatt accaatagaa aagcttatag aaaatttagg tttttcaggg
     6241 tcaaagaatc ttgtgaatat agatatgctt acaaaattag atatatctaa acatgatgta
     6301 aaaactttaa aagttataaa tccttatgca atttatttag taaatagtaa gccatttata
     6361 ttatttatag agtcttcttt tgaagaaaaa aaattacaac aaattagtaa gcttatatgg
     6421 aatgctcaaa taccaattgc ttttatatgc gattctaatt ctgttaaaat atttaatgga
     6481 aaagctttgg atttaaagtc atatttaatt gaggaaatat ataatcaatc tattgaggat
     6541 ttttcggaag aatctgagtt ttcttatttg aaaatatctg atccattatt ttgggatcaa
     6601 tacactaaac aatataccaa aacgcattta aatgattatt tgctagataa tattacttca
     6661 ttgacaaata aattaaagaa aaaatataag gtgaaatttg caacgaagct tgttctaaga
     6721 ttaatattta ttagatattt aatcgataga ggtgtcgata tagcttataa aaattttaat
     6781 catgatatta aagcttcaca aaaagaactg ctaaagatta ctaaaaacaa agttgaatta
     6841 tatgaactgt ttaaatactt aaaagaaaag tttaatggta atttatttga gttaaatgac
     6901 gaaattaata atacttgttt aacagatgaa gtgtttattt tactatctga ttttttatca
     6961 ggtaaaataa gtattgacaa tggacaaatg tctttatttt caatgtatga ctttaatatc
     7021 ataccagttg aactaattag taatatatac gaaattcttt taggaaaaga aattcgtaat
     7081 aaagataacg cattctatac tccgaattac ttagcagaat atattttaga taaaacaaca
     7141 cttgaatttc tgaaaaataa taggcaatac aaaattctag atccttcttg tggatcagga
     7201 gtatttttag taaatagttt tagacgtatt attgatatga atttgggtga taaacttttt
     7261 tgtgaagatg ataaactttt aaaagaactt ttattatcta atatttatgg aattgatatc
     7321 aatgaagatg ctatcgatgt tactattttc tcgttatatc taactatttt agattacaaa
     7381 gaccctaaga tattatcaga atttaaactg cctaatttaa aagggtgtaa tttaatagcg
     7441 agtgattttt ttgatgataa taaattggag agtttaaaga aaattaactt tgattttatc
     7501 attggaaatc cgccttgggg aagtgtctca gatggattac atttagatta ttgcaataaa
     7561 aatggctata atgatatgca acaaaacaaa gaaatcagta gaagttttat ttttagagca
     7621 aaagatttta gctctagaaa tacagtatgt tcttttgtag tgcattctaa aatactatat
     7681 actcaaaaaa agccatcaaa aaaattccgt gaatttttgc ttaaaaaaag tcaaatattt
     7741 agtgtagttg aaatgtcatc tgtaagaaaa ttagtgttta aaaatgcaga cgctcctgca
     7801 gcagttatat catttaagta taacgaaagt gaagaaaatt taaataatag aatcatctat
     7861 acatctttaa aaccaaatgt attttttaaa ttatttaaca ttattgtaat tgaaaaatat
     7921 gatgttaaat atatttcaca aaaactatta ttggagaatg attgggcttg gaaaactatt
     7981 gtgtatggat tcagtaaaga tatagatata attatgaata tcaaaaaaaa atatcaaact
     8041 gtaaatgaaa ttattttgaa aaacagtttt gttcatggaa cagggataca aatcaatggt
     8101 aatgaaagcc atgatgcgac acatttaaaa ggaaagtggt atgttgattc aaagaaagga
     8161 atagattcat tctatgttga tataaataaa gggaaagaac ttactcaaga taaaatgaga
     8221 agacctaagg ctaatcagca agaaatattt attcctccat atgtattatt aaaaaaggga
     8281 gttgacacga aaacatatag atatagagca gcattttcaa atgatgcatt tgtatattca
     8341 gatgcagtta caggaatatg tggaaaaaaa gataatttaa ctacacttta ttgcttgact
     8401 ggtatgctta actcttcttt ttactcatac ttaaatataa tgctaggtac atcaattgga
     8461 atagaaagag aacaaggatt ttcaacggaa atttttacgt atccagcaat agaaagtgat
     8521 gaaattgcta atctggtagt aaaaatacaa gaagcgacta aattagaaaa aacaactatg
     8581 ttcttgaatg aaaaaaatag ttgtaagtat atcaaacaat tagataagtt aattttgaag
     8641 tcttttaatt taaatgatga taaatttatt gactatgcaa taaatattca aattcctgag
     8701 cttacaaatt ctaagtcaac taacatatat agaaaaataa ctaaggaaga tttaattaat
     8761 tatagtgaat gtttcaaaga acagttttca tcgatatatc gtagggttgg gaaaagtatt
     8821 tcaatttcgg tatatccaaa aatattaaat cgttttacag tatttgaact agaagtaaca
     8881 gatgaaaaaa cagatagaga gattatggtt aaagcagaag ctgataataa tatggagttg
     8941 tttacgaaaa taattagata tgattataat gatgtttttc atcaaattag agatattatt
     9001 cattttggag aaaattcatt ttatatcata aagcctaatt gctataaata ttggcatcct
     9061 gctatcgcag aaatggatct tgctgatgtt ttggatcaga ttttatctga tacagggggt
     9121 gaagaataat gggaggattt aaaaattctt cactaaactt ttatttaaag caagggcttt
     9181 atgaaataat tatagatgga ttaatagact gttgtttact tatgagcaaa tcatgtattg
     9241 aacaatctag aaaaatatta aatcacgaag aaaagataag aacgcattta gttgaaaatt
     9301 atttagaaaa tgatattgaa agaagaaaaa catctttatc taaacaacat ctaagatttg
     9361 aagtagaagc acaagaaaat tatgacaaca aaaatgaatc ttatgagggt agggttgata
     9421 taaaagttat atctaaagac actttagcgg atagaagggc ttattatatc attgaatgta
     9481 aaaggataga tggtaaggaa actttaaata aaaaatatat aaccgaaggt gtacaacgat
     9541 ttattactga gaaatattct tcgcattatg gcaaaaatat aatgcttgga tttatagtaa
     9601 aagatataaa tatccagagc aatatacaaa aaattaattc actgaatcag aacatattat
     9661 atacatatat agatacagat atgttgacag ttagaagtga ttctgaaaat aattatgctg
     9721 tttgtgaaag taaatataaa gtaaaatcta aactactatt gttaaatcat attttttata
     9781 acttttcgag tataataaga taatcttatg aatataaaat attactatgt taaaattata
     9841 gtttggtttt gataatatta taaaaataag tttaagaata aaatataaag gaaaaaaggg
     9901 gcgtagcact attttttagt gtgacagccc ctttaatttt aaaatcggta aggaggagtg
     9961 tatttgaaaa aaattaagtc atatacaggg attttgaatg tggagaaggt tatatatgca
    10021 ataaatgatt ttcagcttcc atttccagtt acttttacgc agatgacatg gtttgtatta
    10081 acacttttta taattatatt atttggtaag ttacctccat tatttatgat agaaggagca
    10141 tttttaaagt atcttggcat accaattttt ataacttagt ttatgtctca aaagactttt
    10201 gatggaaaga aaccatatgg atttttaaag tcaataatac tatattcatt aagacctaaa
    10261 ataacatacg caggaaaaaa cattaaattt aaaaataaaa ttattgatga aaatataact
    10321 atagtaagga gtgaattata tgttcccgat aaaatatata gagaacaatc ttgtatttaa
    10381 taaggaagga gaatgttttg catattatga attaatacca tataattatt cattcttata
    10441 agctgaacaa aaatttattg tacatgataa ttttaggcaa ttaatatcac aaagtagaaa
    10501 tggtaaaatt catgcacttc aaatagccac tgaaagttct ataagagtta cacaagaaag
    10561 gttaaagaaa aatattacag gaaaattaaa agaaattgct tatgaaaaaa tagacgagca
    10621 aacagaggca cttgtttcta tgattggcga taatcaaatt gattatcgtt tttttattgg
    10681 gtttaagctt ttagttacag agaaagaaat gaatataaag agtataaaaa agtcaatatt
    10741 agctaatcta tcagatttta tatatgatgt taataataaa ttgatggatg actttgtcag
    10801 tataaataat gatgaaatta tgagattttt aaaaatggaa aagttattag aaaataaaat
    10861 ctcaagaaga tttaaagtta gaaggttaga taaaaatgat tttggatatt taattgaaca
    10921 taattatgga aagaaaggca cagtttttga agattatcaa taccatttac ctacaaagaa
    10981 acttaaaaga gaaacattag taaagaaata caatcttata aagccaacta gatgtcttat
    11041 agaagagaag caaagatact taaaaattat acatgaggat tcagaaagtt ttgtagctta
    11101 ttttactatt gaaattatta ttggagaaat ggattttcca tctagtgaga tattctatta
    11161 tcagcaacaa cagtttgcat ttcctattga tactagtatg aatgtggaaa ttgtaacaaa
    11221 taaaaaggct ttaactacag ttagaaataa gaaaaaagaa ttaaaagatt tagacaatca
    11281 tgcttggcag tcagggaatg aaacagtaac aaatgtagta gaagcattag aggctgtaaa
    11341 tgagcttgta aataatttaa atcaaacaaa agaatcaatg tataagttaa gctatgtaat
    11401 tagggtatca gcagatagca ttgatgaatt aaaacgccgt tgtgatgaag ttaaagattt
    11461 ttatgacgat ttaaatataa aattagtacg cctaatggga gatatgatag gatttcacaa
    11521 tgaatttata ccttcaagca aaaggtatat aaatgattat attcaatatg taactagtga
    11581 ttttctagca ggattagggt ttggagcaac tcaatagctt ggagagaacg aaggaatata
    11641 tattggatat agtttagata caggaagaaa tgtctatctt caaccatctc ttgcaagtca
    11701 aggagtaaaa gaatctgtaa ctaatgcgtt ggtagcagca ttctttggat ttttaggagg
    11761 tgggaaatca tttgctaata acttacttgt ttactattca gtattgtttg gagggcaagc
    11821 tgttttggtg gatccaaaag ctgaacgtgg caaatggaaa gaaaatttac cagatatata
    11881 tcatgaagta aatattgtta atttaacaag tgatatagaa aataagggaa tgctagatcc
    11941 ttatgtaatt atgaaagaag taaaagatag tggaagtcta gctattgata tattaacttt
    12001 tcttacagga ataagtagtc atgatggaga gcagtttcct atacttagaa aagcaatcat
    12061 aaatgttact caagacaaat gcagaggact tttgaaggta atagatgaat taaggaaaga
    12121 taattctcaa atatcaaata atattgcaga tcatattgaa agttttacag attatgattt
    12181 tgcacaatta cttttctcag atggaaatat taaaggctca attaatctag aaaaacaaat
    12241 gaatataata caagttgcag atcttgtttt accgaataaa gatactttat ttgaagaata
    12301 tacaacaatg gaaagtcttt cagtagctat gttgatagta attagtactt ttgcattaga
    12361 ttttatccat acagatagaa gtacttttaa aatagtagat ttagatgaag cttggacatt
    12421 tcttaatgta gcacaaggaa aaactttatc taataaatta gttagagcag gatgtgctat
    12481 gaatgcagga gtgtattttg taactcaaaa tgcaggtgat gtatcagatg aaaatttaaa
    12541 aaacaatatt ggattaaaat ttgcattcag aagcacagat atcaatgaaa ttaagagaat
    12601 acttgaattt tttggagttg ataaggaaga tgagaataat caaaaaaggt taagagagtt
    12661 agagaatgga cagtgtttaa tacaagattt atatggacga gttggggtaa tacaaataca
    12721 tctagttttt caagaactcc ttactgcatt tgatacaaga ccacctataa gaaaagaggt
    12781 ggattaatga aatcgaaagt atttagaaat ttaagctgga taactatttt aatagtagta
    12841 cttttactat tttttattta tataaattta tagatttaga gaaacaaata gatacattag
    12901 ataagaaaag aaaaaaactc atagatatga gattagaaga tataattgat aaagaatcat
    12961 atgaagaaaa gtatgatgat ttaacagttg aaataaagaa acttagtaaa gagaaaaaaa
    13021 ctcttaaagc atcattaata aatgaaaaga gtataaaagc aagacttaga gaatttagaa
    13081 agatacttga aagaaatgaa ttgttagaaa aatttgatag atatgtattt gcaagtatag
    13141 tagataaggt tatagttgga gttattaatg aagatggtat agttgatcct tataaaataa
    13201 cttttgttta taaaactagc tttgatgata aacaagatgg acataaattt ataaataaga
    13261 gtccgttaga aacgtattca aaagataaca acgataataa aaagaagtgt tcatatacta
    13321 ataatgagac attgcgagac tgtagttaaa cttattagag aataacaagg tttcagactt
    13381 ttatattgtg tcttaaaagt agatttgcca cctatttgcc accataatta tttttgggtg
    13441 gcaaatttat tctacaatga tttcaaaaat atttaccgtc tggtttctca tgttgttagt
    13501 tacatgtaaa tatgtatcca tagttgttga agttttagca tgtccgagta tatgctggat
    13561 atcttttata ttagtaccat tttctaataa catagtatca gggtttttaa aataaagtgt
    13621 gtattcttct aaagtagtga taattaaaat atcaaatatc actatacaaa ggaaagtgca
    13681 cgctttttat gtttaacaaa aacgaaatat taaaaaactt attgaagaat atgatgtcaa
    13741 aacaactaca gatattcaag atatgctaaa agatttattt acaaatacaa ttcatcaaat
    13801 atttacattg tcgaatttta gcggtttaaa cataatatat tgactgatat aataaaatca
    13861 tagaatattt tagggggaaa tagattatgt ttaagaaaaa gttaatagct gttgcaatga
    13921 gtataactat gattagtgta gggagctttt cgtatgctca ttcaggaaga actgattcat
    13981 caggaggtca tcgtgataat aaaaataaat ctggattagg aagttatcac tatcattgtg
    14041 gtggttatcc agctcattta cataataatg gtggttgtcc ttatactgga ggaggaagca
    14101 gttctggaac aacaacttcg gttaataatg aagaaaagca aaagaggtct gttggagaaa
    14161 aaggatataa tcaagggtat gaagatgggt ataaaggaaa ttatagttca tcaaattata
    14221 gtggagatta tagtgataca tatgaatcaa agtactcaga aggatatgaa aaaggtaagg
    14281 ctaaattaga agaggaagaa aaggtggcta aagagactgg atataattta gggcttacag
    14341 gtgctaaatc taataataca tatgaaaaag aagttttaaa aaatgcttat gataccggat
    14401 attcaactgg atacaatgaa tataagacta aaaagataga agaatataaa gctaaaggga
    14461 tagaggatag caataaagat aaggaaaaaa tgacatttga agaaaacata gattctgaat
    14521 ttatagatgc ttataataat gcttatgacg aaagacaaga acaattaaaa aatgattaca
    14581 ctactcaagg atttgagagt gctatcaaag gagaaagatt tgacacatca actataggta
    14641 atgttaaata tgctaattgg tttaaagaag gatatgataa tggtaaggtt aagttaccta
    14701 aagcaaaaga aagtgtatat aatcaaggtt acaatgaaga agattttagt ataccagatg
    14761 aaatgaagtc tattgaaact aagttaaaag gtgtatatga tgaaggttta gaaaaaagag
    14821 aagaagagaa aagtagaaat gctacttatg gggttggagc tggaacagca gctgtagttg
    14881 ctggagtagt atataagaag aaaaaacgta aaaagatata gatgctagat ataccaatga
    14941 tgacaagtgt ataacaaata taattttaag tataaatatt taaaatatag aagggaaggt
    15001 tatatctatg ggatttaact ttaggaaaag cataaatcta ggaggaggac tcaaattaaa
    15061 tttaggtaag aagagtgtag gtataagtgc tggtgttaaa ggtgctagag tgtcagttaa
    15121 ttcaaaagga agaaaatcag ctacattgag tatacctgga actggaatat catatacaaa
    15181 aactagtaca aagaaacgta aacctaaata aatatttgat atactaagaa atagactgtt
    15241 acaaatagtc tattttttag tatatagatt taaaaagtga caaaattttg aaatataaac
    15301 taatttcata aaggaaaata ttattttatg tagaattata atatttaagt aatattataa
    15361 aatttatagg aggatttcat tatgaaatta agcaaaatac tattggcaat agggatgtct
    15421 gtttcattat tgattgctag ttgtccaaca tcaaacgctc ttagttctat taattcaata
    15481 aaaggtacaa ataagtatga aacagcaggt ttaatagcag ataaacaaaa ttatgatatt
    15541 gcaatactta ttaattctga aaatggatta tctgatggat tgtcagcaag tggattgtca
    15601 ggagctaaaa atgccccaat attgttaact aagaaagata caatcccttc agaaacatta
    15661 aaaagattag aaaaggtaaa agaagtatat ctaattggag gaaaaaattc gataggagca
    15721 tcagttcaga aaatattaag tgataaaaaa ataagcataa atagaataag tggaactgat
    15781 agaatagata caagtcataa agttgcaaaa gaaataggaa gagtaagtac ggttaaaaaa
    15841 gtaatattaa ctaatgcttt taaaggcgaa ccagatgcta tgagtgttgc ttctatagca
    15901 gttagagata aatctccaat agtattaact gatggaaata ctacaagttt tgctacagat
    15961 ggattagaaa cttatgcaat tggtggaaaa gtcagtatga gtgataagtt aataaatgaa
    16021 actaaagcaa caagaatagg tggagtagat agatacgata caaataaaca aataataaat
    16081 aaattttata atggctcaaa agaattttac atagcaagtg ggactgattt agtttattct
    16141 ttagttggtt ctacaattgc acaaaaaaca cctatagtgt tagttggaaa tggaagtaat
    16201 aagagtatat taaaaaatgc aagtaaaata actgctattg gaaatattga tgatgttgtt
    16261 ataaaacaat gtttaaatat aactaataat gttggggatt caaatacagg tattgtaaaa
    16321 gatactaatt caaataatac gaacaaacca agtaatgagc ctagctctaa taatcctgga
    16381 ttaactgata aaagtacagt atatgtaact cctaatggaa aatcatatca tctaacaaaa
    16441 aattgttcta cactaaaaag aagtaaaaca ataaataaag taacattaaa acaagctaag
    16501 tcacaaggta aaatagatcc ttgtaatgtt tgtgtaaaat aatgtattag ccaaatatat
    16561 attaaaaaag acaattaatt aagattgtct tttttattga agaaatataa atttatatta
    16621 tgtattatta aaaaaaaatt attaattata aaaaataata ataaagacga taagtattaa
    16681 aattttatgt ttccaatatt tatgatataa ttaatatggc aaggaataaa tatttgaaaa
    16741 agctatgagt ggtgtttcca tggaagtttc ttcactttct tatgagagga ggtgaaaact
    16801 atggaaaatc taatgatgag tattataact ggtgttatag ccagttacat ttacgataaa
    16861 ttaaagagcc actctgagcg accaactaag agtggctggg aacttaatat aaagttccag
    16921 aaaagtaaat cttcaaaata atttgttgaa ggaaacatca ctcaaagtta agtaaaatat
    16981 gtttccttgc ttttattata tcacaaatta aaaatattaa cacaaaattt tgtgaatata
    17041 tacttattat tgttctaaaa aaatatgtaa ataattgatt tgattgcatg gaattattga
    17101 tgtaatattt aagttaaata tacacaatct caattaaaga agataagtac tataaattca
    17161 tatcaaaaaa atacataaac ttttaagtta taattttata aaaagtcttt tagtatcatt
    17221 atttcaagag gaattatcat atgaaaaaat aacttcatta gaaaagtgga taaaaaactg
    17281 gtaagaagat gactaaagat tacatatact attacttaaa ttaaaaattt aatataattt
    17341 ataatgtata caatttatta taaaa
//
LOCUS       DAELLV010000060        17208 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.060, whole genome shotgun sequence.
ACCESSION   DAELLV010000060 DAELLV010000000
VERSION     DAELLV010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17208
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.060"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(142..666)
                     /locus_tag="KPD58_001627"
     CDS             complement(142..666)
                     /locus_tag="KPD58_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG0265401.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQVMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(731..1483)
                     /locus_tag="KPD58_001628"
     CDS             complement(731..1483)
                     /locus_tag="KPD58_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBG0265402.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDVSKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(1514..2812)
                     /locus_tag="KPD58_001629"
     CDS             complement(1514..2812)
                     /locus_tag="KPD58_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HBG0265403.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVESTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEGIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMEDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQHGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREANVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(2829..5093)
                     /locus_tag="KPD58_001630"
     CDS             complement(2829..5093)
                     /locus_tag="KPD58_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HBG0265404.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(5348..6166)
                     /locus_tag="KPD58_001631"
     CDS             complement(5348..6166)
                     /locus_tag="KPD58_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBG0265405.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGMDEKTIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIVL
                     LDEPTAGLDPVSTRSIVDIIKGLNKNNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFINENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(6159..6857)
                     /gene="cbiQ"
                     /locus_tag="KPD58_001632"
     CDS             complement(6159..6857)
                     /gene="cbiQ"
                     /locus_tag="KPD58_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HBG0265406.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIQNIFILSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEFFSFN
                     VGISKASIDMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKLHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(6860..7138)
                     /locus_tag="KPD58_001633"
     CDS             complement(6860..7138)
                     /locus_tag="KPD58_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG0265407.1"
                     /translation="MSAKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEITK
                     INPNYKPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGLQKGKRS"
     gene            complement(7128..7880)
                     /locus_tag="KPD58_001634"
     CDS             complement(7128..7880)
                     /locus_tag="KPD58_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HBG0265408.1"
                     /translation="MKQNIKLGVIAALMLIILTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLFGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIVSFLIFKALKKKDG
                     NNAIPVFLAAAIGDLATYTITSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(8062..8254)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(8386..10158)
                     /locus_tag="KPD58_001635"
     CDS             complement(8386..10158)
                     /locus_tag="KPD58_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HBG0265409.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDIILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYSMDKHPALDSMTRRIVNDIRNIDTN
                     QDIMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(10172..11101)
                     /locus_tag="KPD58_001636"
     CDS             complement(10172..11101)
                     /locus_tag="KPD58_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0265410.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            11329..11517
                     /locus_tag="KPD58_001637"
     CDS             11329..11517
                     /locus_tag="KPD58_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0265411.1"
                     /translation="MEYMSCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKN
                     AEKPVDGRTKRRKEVQYE"
     gene            11510..12211
                     /locus_tag="KPD58_001638"
     CDS             11510..12211
                     /locus_tag="KPD58_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0265412.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAIIAHCGAEGLHLVDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDCIIINGMIIDKTNRIVSVNNIPVQLTS
                     KEFDLLAFLASNKGQVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            12213..13136
                     /locus_tag="KPD58_001639"
     CDS             12213..13136
                     /locus_tag="KPD58_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0265413.1"
                     /translation="MNTSVYLLLILVLSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            13293..14216
                     /locus_tag="KPD58_001640"
     CDS             13293..14216
                     /locus_tag="KPD58_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0265414.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHVKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKEREKQVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSKYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDYTLHLYNTNLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            14209..14946
                     /locus_tag="KPD58_001641"
     CDS             14209..14946
                     /locus_tag="KPD58_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0265415.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPIKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFANIALGRRK"
     gene            14957..15730
                     /locus_tag="KPD58_001642"
     CDS             14957..15730
                     /locus_tag="KPD58_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0265416.1"
                     /translation="MWKLLWAECQKIRRSKIVWITVFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETNLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIISLLLCACLSAFILKGLKHNEKN"
     gene            complement(16257..16865)
                     /locus_tag="KPD58_001643"
     CDS             complement(16257..16865)
                     /locus_tag="KPD58_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBG0265417.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
     gene            complement(17043..17159)
                     /gene="rrf"
                     /locus_tag="KPD58_001644"
     rRNA            complement(17043..17159)
                     /gene="rrf"
                     /locus_tag="KPD58_001644"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         5539 a         2989 c         2173 g         6507 t
ORIGIN      
        1 tatagatttc cacaatacaa ataaattaaa aagtgaaatg tatttgtcta aaaacataat
       61 aacatttcac tttatcctca tttttaagta atatatattt ttaattttta tttataacta
      121 aatctagttt ttatataaat tttagttttt ttctacatct gagtaatcta ttccaaaagt
      181 ttctacagta acagatttca taacttgtgg agtttgtggc ttatcagctg aatctgttct
      241 aactgatgct atttcatcaa ctgtatccat accttctatc actctaccaa aacctgcata
      301 ctgaccatct aagtgtggtg agtttttatg cattatgaaa aattgagaac cagctgaatt
      361 aggtgccata gttctagcca ttgatatgac tcctctttca tgttttaaat tgttagtaaa
      421 tccattacca ctaaattcac cttttattga gtgacctgga ccacccattc ctgtaccatt
      481 tgggcatcct ccttgtatca taaatcctgg tattactcta tgaaatatta ttccattgta
      541 atatccttca tttactaatg atacaaagtt tttaactgta tttggagcta tatttggata
      601 taactcaatt ttgatttctt ttccattttc catttctata gttactatag gatttttatt
      661 ttccataatt tattaccttc cttttcatta gttaaattac ataattacat ttattatatt
      721 atactctaaa ttaaagtcta tcaagtatat tttttcctat ttcatatcct tcttgataaa
      781 tagttttaca aaactcatca tcaaagttaa gcgtgtcttt aggagcaaat tgagtattag
      841 gtctaactac tattatttta tcttcatcat atactttttt aatatcttct ggcaactcat
      901 aattatattt tgtggatatt aaaataattt tatccacatt atccccaatt aatggttcaa
      961 ttaatgcact cctaattaaa cctccatcta atttgaaacc atcataaatt ccttcttgca
     1021 catctttcac aaattgttct ttaaatccaa gagttgcgtt tggatttgat ggtaacaacc
     1081 cactgtatga aatgctgata atggcttcat tacgaggcat ttttgatacg tcaacaatct
     1141 tttctgatac tactttatta tcaacttcaa cataattaac ataaaagttc atttcttcta
     1201 tattactatc atctagttcc cttaggttgt ctatagcagg agaattatcc acagtattat
     1261 ttactatttt tacatttcca ttctggatat cttttatatt tgtccacatt ttctccaagt
     1321 tatcaacatt ccctgtgtac aaataatatc catttattgc tcctattgat gtccctgaaa
     1381 tcgaataaaa tttatttatt cctccatcat acaaggcttt aactactcct gcttgataag
     1441 ccccttttgc tcctccacct tctaaacata atcctaattt catagtctca tctccaaatt
     1501 gtttatctta tatttatatt tcaaaaaact tactaaatgt tttatatata ctatagtggt
     1561 cctcaacatt tcctaattct cttatagaat gcattgctaa aattggactt cccacatcca
     1621 cagatggtat atctatatga gtagaagata ttggacctat agtacttcct cctctttcat
     1681 ctgacctatt aacaaattct tgatactcaa cattagcttc tctacatata tttttataaa
     1741 tagctataga atatccatca ctagtatatg cctgactagc atttatcttt ataaccggac
     1801 cttttcccat tattggttta ttagttgggt catgcttttc tggaatgttt ggatgaatag
     1861 catgagctaa atctgatgat atcataaaag atgaataaat agactcaaag aatccctctc
     1921 tatccttacc cattgatatg caaattctct ccaatatatt taacaacatg tttgaatctg
     1981 caccttgttt agtagagcta ccaacttctt cattgtcaaa tacagccatt acatttacac
     2041 catgttgacc tttagtatta acaagagcat tcaaactagc atgtaccatt gcaagattat
     2101 cttgtctccc aatagatata aattcatcat tagcccctat taaacttcct ttttcaaatt
     2161 catataaaaa taaatcaaaa tctataatat cttccatatt tacatctaag ttagatgcta
     2221 tttgctttat aagaaaatta tctttttcta atgtatcatt taacatccca actaaaggta
     2281 acatatcttt ttgcttatta tatgaatgac catcatttat acttctattt agatgtattg
     2341 ctagatttgg tattatacaa attggctttt taaagtctat aagcctttca tttggtttta
     2401 atatactatc accttttaaa actactctac cagctatccc aagaggtcta tccaaccatg
     2461 tacttaatat tgctcctcca tagcattctg tatttaactt caaataagat ttttcagctt
     2521 ctatttctgc atttggttta atactaaatg taggagaatc tgaatgagag cctattattc
     2581 taaatccatc tctttctata ccttctgaat ttactacaaa agctactata gctgataaat
     2641 tcttagtaac ataatacttt ccaccaactc ttagattcca tctttgattc aaaactaatt
     2701 cttcaaatcc atttttatct aagatttcct tagtactctc gactgcatga aaagacgacg
     2761 gactatcata tataaagtct attaagtttt tagcaaagtt tatactatcc attcctcatt
     2821 tacctccatt ataaaatatt tagtgttgta tattctttat ttattttttt aattccctgt
     2881 tggtcaaaag ctatagtaac atctgtacca attatagaaa ccactgtccc aaccccaaat
     2941 ttaggatgat gtaccttaga accaagtttt atatcatctg gattagtttc tttattagaa
     3001 tcttttattg tagcattaac tttatctgca actttagatt tgcttatagt actcatatac
     3061 tttttagtat atttatctaa catattataa cttgctttag aatagcttag ttcttttttt
     3121 actttgtaca atctttctat acattcttct ggcagttctt ccataaatct tgaagctatc
     3181 gcaacatttg ttttaccata taatgttctc ttctctgtca atgacatata tagctcttct
     3241 ttagctctag ttataccaac ataacaaagt cttctctctt cttctatttg tgaatcactc
     3301 atagatttta tagctcttga tataggaaat aacccttctt ccatacctgt taaaaacaca
     3361 acagggaact ctaaaccttt ggatgtatga attgtcataa gcgaaaccct atcattgtct
     3421 tcttcatcat ttgattcaga tgtaagtgct atacttgtta ggaatgtctc taagtcttta
     3481 ttttcataag tatcatcatt actactttca aactccaaag ctatagaaat aaattctctc
     3541 aaattatcaa ttctgtcttg agcttcttca cctttgcctg ttaaatcttt ttcattttta
     3601 tttcttatct ctacaagctc gtccatatat cctgtagtat ccaagacttt ttctataagt
     3661 ttgctaacag gataaacttc ttttatagtt ctaagagtcc ctataatatc aacaaatcca
     3721 cttatacttg cccttgcctt agtagatata tctgaatttg tttctatatc aattaatact
     3781 gagtatatac tttcctgttt taagttagct ctatcttcaa ttttttctat agttctaagc
     3841 cctatacctc ttctaggaac atttataatc ctttttatag atatatcatc ttgtggattt
     3901 tgaataactc ttaaatacgc caacaaatcc tttatttctt ttctctcata gaattttgta
     3961 ccaccatata tattgtatgg aatttgactt ctatttaaag cgtcctctac aggtcttgcc
     4021 tgtgcatttg ctctataaag tactgcaaaa tccttataag gtctattttc ttttctagct
     4081 atttgtgcaa ttgaatcagc aatgaaatca gcctcttcta tttcgctacc agttaattgt
     4141 atctttataa gttctccttc ttttttttca ctccaaagct tttttctttt tctttctata
     4201 ttattagata taactttatt agctgcatct aatatcactt gtgtagacct ataattttgt
     4261 tctaacttta caacatgtac attgtcataa tctttctcaa attcaagtat gtttcttata
     4321 tctgcacctc tccatccata aatactttgg tcatcatctc caacaacaca aatattttga
     4381 tgttctcttg ctaataattt tatcaattca tattgagctt tacttgtatc ttgatactca
     4441 tctaccatta tgtatctaaa tctacttctg taatatgcta atacttcatc attagccttg
     4501 aaaagttcta cagttttaag tattaaatca tcaaagtcaa gtgcactatt tctttttaaa
     4561 cggtcttgat atagcgcata aatatctgca atcttaacca ttctattatc tgccatattg
     4621 atatctttaa actgctttgg agtataaagt ttgtcttttg ctcctgaaat tgcacttata
     4681 actgcttttg gttcaaacac tttatcactt aaatttaatt cttttagaca gtctttaacc
     4741 aatgtaactt ggtctgctga atcataaatt acaaaacttc tattgtaacc aatcttattt
     4801 atatcttttc ttagtatacg aacacagcaa gaatggaatg tacttatcca catatcctta
     4861 gtttcaggac ctatattttg ttcaactctc tctctcattt catttgcagc tttgtttgta
     4921 aatgttatag ctaatatatt tggtgcttgt acacctttat cttctataag atatgctatt
     4981 cttgttgtta ataccttagt tttacctgaa ccagcaccag ccagtataag tactggacct
     5041 tcagtctttt ctactgcttc tctttgagca ggatttaatg tatctaaatt catatttctc
     5101 ctcctgttaa cagccataaa tatattttaa tctaaaatat gaattacttc aaattataat
     5161 tatgtattat attaatagat tatttccaat atattacaat taaataacat ttttccatat
     5221 aatttatttc attgtttaat aaaaaaaata accttatgat ttggggaatc ataaggcacc
     5281 tttaaggggt ctaggttaag ggttattata tataaatcta tttttttaaa ataggtttag
     5341 ggcttaatta tttatttgta ttaagttctc tttccttcca atacttatac aaatcttctt
     5401 ctttcttaaa taagggtaaa ttcatatttc tatgtacttt tactaaccat ggggattcta
     5461 ggccagcttg tataatatta ttttcattta taaacacatt ttctgctttt ccttcatcaa
     5521 tcaaaatccc cttatccaat acatatatgt aatcacatat ttcatacatt aaattcatat
     5581 catgactgga tattactatt tttatattat ttttatttaa tccctttatt atatctacaa
     5641 tagacctagt acttactggg tctaaaccag ctgtaggttc atcaagtaat acaatttcat
     5701 tttccattgc tataactgat gctattgcca ctcttttttt ctgtccatag ctaagaaaat
     5761 gtactggcct gtctataaaa tctattccat taacagctac tagagctttg tttattctct
     5821 ctttaattgt tttttcatcc ataccaatgt ttctcattgc aaaggctata tcatcatata
     5881 cttttgaata aaaaatttgt ttttctgggt cttggaagac aatacctaca ttttttctaa
     5941 ggttgtataa acttctctta tcatatttta acttttcttc tttaaaaaga atttctccag
     6001 atgtaggctt taatataccc attaaattca taaataatgt tgattttcct gaaccatttg
     6061 aacctattat acctataaca tttccctttg aaaaatccat attaatgttt aatagtgcat
     6121 tggtattttt ttcatattga tacgttaaat tatttatttt aaacatcatt atcccctact
     6181 atgtgaaatt taccatcata caattttatg tctaatgata tacacatatc atcatacctt
     6241 gtcataagtc ttttgtacag catacttcca agaataccta aggatttata tgaagttttt
     6301 aaatttatat atccaaatct tagctcttgt gacttgtata tttctgaaaa ttcttctaaa
     6361 aatatgaata taaacctata tattaacatt gataactcga caaaagtgtc aggtaaatga
     6421 agttttttaa aaaagaatat taattgatta aaaggtgttg taagtataca aaaatacaca
     6481 caagtcaaac aagacatcgc tctaaataac acatgaatag acatgtcaat agacgccttt
     6541 gatattccta cattaaaact aaaaaattca aaactatata ataaatctgc tttattaaaa
     6601 gatatattta caagagttat tcctatacta agaaataaaa agtacattgg tattctaagt
     6661 aattttaaat aattttttaa atctattcca gccacacaaa caaccaaaat actcattaaa
     6721 atcataattc cacttaaaat aaatatattt tgtattacca tagaagcaat cagaaaaatt
     6781 actcctatgg ctactttttt gtttggattt actttactca atctatttgt atatgcatat
     6841 ttatcaatta taagcatgat taacttctct ttcctttttg aagacctaat atgtatccta
     6901 ttacaccagc ccctaaagct gcttgtgcag agaataataa actttctatt tctccacttg
     6961 gtggctcata tggactgcta aaccatggtt tgtaatttgg atttatctta gtaatttcac
     7021 tttcagcttg accatctgct cctccatatt cagcacctga gttgacaagt aatgggaata
     7081 taataagagc tactactaat aaaagtaata atatgttagt ttttgtttta gcactcatta
     7141 gctttaactc ctttatcatt attttctagt atgctttttt ctccgttttc tgtaattaaa
     7201 ttatatacaa ttacagtaag tataccttct gctattgcta ttggtatttg agtcataaag
     7261 aaaattccta aaaatttaat tgctgaagcc attaccccac ctgatgggtc tgggaaagcc
     7321 agtgctaatt gtattgaagt tattgtatat gtggctaagt ctcctattgc tgctgcaaga
     7381 aatactggaa tagcattatt tccatctttt ttctttaaag ccttaaatat taggaatgat
     7441 acgattggcc ctattatcgc cattgaaaaa gcattagctc ctaaagtagt tattcctcca
     7501 tgtgctaata acaatgcttg gaatattaaa actatagttc caagaacaaa cattacgctt
     7561 ggtccaaaca ttattgctcc aagacctaca ccagtaggat gtgaacaact tcctgttact
     7621 gaaggtattt ttagtgctga taatacaaac acaaatgctc cacataaagc taataatact
     7681 ttctttttag ggtcttgctt tactatttta cctattgatt ttaatccgaa taagaaaaat
     7741 ggtataaata taactcccca tgctatagac cattttactg gtaagtaacc ttccattata
     7801 tgcattgcat ttgatgtaac tggagttaaa atgattaaca taagtgcagc tataacccct
     7861 aatttaatat tctgtttcat cttagtatcc ctctctccct ttctaataat taaaacataa
     7921 taatacatgt ctgatttatt taataaaata aaaaagccta actcgatggt taggcttcta
     7981 attttccaca atctaaatac aaacacataa aacaaacata tctgtataat tattataaga
     8041 aactttctcc atccccgaag aaattacttt aatattttag gcaggtctcc tgacttagct
     8101 tcatcctact cctcttcctt cccatgagat ataaaaaata tcatcatagt ggctaacaaa
     8161 agtttcgtct gcatcacagt agcgggggct gtaaaggaat ttaacctttt tccctattaa
     8221 tcttgcttaa agaacctata atattatata tttaattttt aaattcttct taaattattt
     8281 tctatataaa tcattgtata ccttttctgt gaaaatttct acatttttta taaattttat
     8341 tcatttttat atttttatgt atataaattc ttttttgtaa gtttattaat ttctaattat
     8401 cttctttaaa tacattgaaa ctacatcaga aaatctagtt atatctcttg aatatataaa
     8461 atcatttcca tatattaatc tttcagcttc aagttctgat tcttttcctg taaatactcc
     8521 taatactaat atattttgct ttctagcttt tctcacttca ttggctgtat ctctcaaacc
     8581 aactataccc ctataagcct tttctcctct taatgttctc tcactacttt ttcctatctt
     8641 tacatcattt ggtcttccat cgctgagaac tatcaggatt ttattttctt catctctatt
     8701 tattagacca ctacatatag attttattgc caaaccatct ctattattac cagcacaaaa
     8761 atattcaaat atattttcac tattttttat actatcatca taatctttaa atctttttaa
     8821 tattgtatag tctaaaaaac tagaaaatcc catcactcta tttggtatgc caacttctgt
     8881 tagagcattt gcaattatgt aagcttgaat tgccacattt ccctgatttc tactttgaga
     8941 gccacttgca tccaataata tatcaataac gtatttccct ttttcatttt ctatatcttt
     9001 ataaaatatt ttattattat tacttctacc aactctccaa gctctatttg ctaatatagt
     9061 accacaatca gaatatactc tacttttagc attttcttct attaaaattc ttgatagact
     9121 ttcttttaat ctctttattt gctgtctata cattctagca tgttctctaa attttcctaa
     9181 attattttct ttttgtcttg ttacatattt tagttgaaat acattattac actcacttct
     9241 aagaactcca tctgtaaagt gtattctaca accttcatga acatttctac aattttgtgt
     9301 ctcaattttt ttaatttcac ctttactcaa ataagttttt ccataataat gttctatttt
     9361 tgaatatatc ttatttgcag tgtcttcatc aacatatata actcggtctg ctgaattgct
     9421 acatttagaa tcatcaaaat cccctacacc ttctacaagc atggatgaag atattgaatc
     9481 tatatcactt tcaataatat tttcttcttc atcactataa agttcttcat acatgaaatc
     9541 agcaaatgta tcaaaattta cttctacact ttctacatta ttttcatcaa taagactatt
     9601 ttcttctcca tttaatatgt attcaaaata agttaaatat ataacatcta atgcttttaa
     9661 aatctccata atatcttgat tggtatctat atttcttata tcattaacta ttcttctagt
     9721 catactatct aatgctgggt gcttgtccat actataaagt acaaatgtgt atttaatttt
     9781 atccataata ttatcattgt gtattttggt aaaacgtgaa acaatatctt tataagcatt
     9841 tcttcttatg tcttctatcc ctggcctatc tttaatgacc ttttcttcaa ctattgtatt
     9901 taatataact tgaattagac ccagtataat atctttgttg tatccttttc ttattctatt
     9961 tgtaagatat tttttaacta tatcccaatc tacatacttt cttctagcac ctgcaattat
    10021 tgcaaaatat agacctattt cttttgaaga gtaatctttt tctgatatat ctatactttc
    10081 attgtaatca ccagctattg tccatgataa gttattagct ctattttcaa attcataatc
    10141 atcatatatc catttcataa tctcacctat attaaatatg acttattgga aacacatcta
    10201 tactctccca tttattaggt attctagttt taattacatc tccaaccatt tctttctcat
    10261 atacatcaaa agtctttcct gtaagtccca tattaattgc aagagttggt tttaatcctc
    10321 ttcttatagt cttaagagat gccataagac cacgtaaatc aatagcttta ctagatatct
    10381 ctccattttg agacttcaat tgtaaatcta aaaatattcc tgcaaagtgt attagttttt
    10441 cttcatcagc atctgaaaat tcatttttta aaatcatcat taacttatct tcttctatag
    10501 gaggaatatc tattaccata aatctagata ccagtgcttc attaagttct tttgttcctg
    10561 catattcata attcatagtc ccaataaatc ttgttgctgg atgtagattg actctctcat
    10621 acccaggtac atcaattatt cttctatagt ctaaagctga atgtaccaca actatagcat
    10681 cattttttgc catattaatt tcatcaaaca ccccaaaacc tccattaata gcacactcat
    10741 aaactgagcc tcttctaagt ttaacttcat tgtctataaa tgtatctgta cctataagtg
    10801 ttgaactgtc tgtatttata tgaaatgatg tattccactg aggtctatta aatatttccc
    10861 ctagattatc agcaagtaag ttttttcccg ttgcctttgg tcccgataat aatatatttt
    10921 cttcttctaa aatagctgct atacacatag acaagatatc tttaccataa aaataagttt
    10981 tagactttgt tactctgtat tctaaatctt tttctaatcc ataataattt ctaaaataca
    11041 gtacatcttc aataagtttt tcatctaccc cttgcaccct tagattatct attatatcca
    11101 tatttttacc ccttttatat aaatagtatt tgtttcattt cttatcacca ttttagcata
    11161 aaaggatata agctaaaatt gattttttaa tctccccatt ttaagtaaaa ccatgcacca
    11221 ttttatcact acaaaattaa gagtactact tactgatatt tcttgatttt gttcgtctat
    11281 acgtatataa tatatgctga taaattatga ggaaaggatg attgaaatat ggaatatatg
    11341 tcttgcccag aagtagcaaa aaaatggggt atttctgaaa gaagagtaca aaaactatgt
    11401 gaagaaagtc gcataccagg agtttcaaaa ctcgggtata tgtggctgat accaaagaac
    11461 gcagaaaagc ctgttgatgg tagaacaaaa agaagaaagg aagtacaata tgaatagcgt
    11521 tttgattata gatgatgaca aagaactttg tgctcttatg aaaaaatgtg ttgagcaaga
    11581 aaatttatct gctattattg cacattgtgg tgcagaaggt ctacatttgg tcgataaaaa
    11641 tagacacaca ctctctcttg taattttgga cataatgatg ccagatatag acggttttca
    11701 agttctgaaa aaaattagag aaacaagtaa tattccagta attatgctta ctgctaaaaa
    11761 tgacgaggat gataaagttt ccggactacg tttaggtgct gacgattacc taacaaaacc
    11821 gttcagcata aacgaactta tggctcgtgt caattctctt attcgtcggt atactatgtt
    11881 aaaccctgct tttataacag acacagattg cataataata aatggcatga ttattgacaa
    11941 gacaaaccga attgtttctg tcaataatat tcctgttcaa cttacaagca aagaatttga
    12001 tttacttgct tttctggctt ctaataaggg acaggtgttt accaaaaaac agatttatac
    12061 acaagtatgg gaagaagaat atgcttttga cgatagtaac atcatgtctt tcatcagtaa
    12121 attgagaaaa aagattgaac cagaccccaa ccatccattt tatattttga cagttcgtgg
    12181 tgtcggttat cgttttaata aggaggctta acatgaatac aagtgtctat ctgcttttga
    12241 tattggttct tagcctgatt attatagcgt accttgtttc tgtcctgcta cgtgtccgaa
    12301 tacaacttac acttataaaa gatgctttag aggatataaa aaatggaaac cttaatcgac
    12361 gtattttaac agatgaaaac gacattacaa aacaaatttg ctatgatatt aacgaaatag
    12421 caatcaatag ccaatttcaa cttattcagc aaaaacaatc agaacaagcc tacaaacagc
    12481 taatgacaag tctctcccat gatgtaaaaa ctccacttgc ttctttggtc ggttatttgg
    12541 aagcagtaga aagtaaaatt gttgtaggag aggaaaaaga cgagtatatc catgttgcgt
    12601 ctaataaggc tcattactta aagaattttg tagaaaatct atttgaatgg gtaaaattgg
    12661 attctaagga acaagttttt cattttgata tttttgactt gaatgaatta tcccgtaata
    12721 ttatatctga ttggatacct gttttagaaa gcagtcattt tgaatatgaa tttgatatac
    12781 cagaaataga gtacttctta cgtatagacg caaatgcata tacccgtatc attaataacc
    12841 tattacaaaa tattattacc catagtagtg gcaacaaaat gacattgaga atttttgaaa
    12901 acaaagagca agctcaaatt ataataacag ataatgggaa aggtatatct tcagacaatc
    12961 tcccacatat ttttgaaagg ttgtaccaat gcgaccattc acgagcttca aagggaaatg
    13021 gtttaggctt ggcgattgca aaggaactta ttaacgctca taagggaaat attaccgcca
    13081 acagcactcc cggtatggga acagaattta caatattgct tcctaaagcc ctgtaaaagt
    13141 acgaggcttt cttactctaa aacaagataa aagcaagatt atggcaaggt tttggcaagg
    13201 ttcatgtttt ataattctaa ttgtagttga ttttgacgtt gtatcaacta tcttttgtag
    13261 aaacaaattt attaaaagtg aggaaattat atatgaatga ttttgtgatt gaaacaaagc
    13321 aacttacaaa aatttatggg aatcaaacag tagttgacaa agtcaatctc catgtaaaaa
    13381 aaggccgaat atatggtctg ttaggacgca acggagctgg gaaaaccaca attatgaaaa
    13441 tgattttagg attgacttca atcacgtatg gcgaggtaga tgtatttgga gagaatatta
    13501 aggaacgtga aaaacaagta tatccccgta tcggtgcaat tatcgaaacg ccaggatttt
    13561 atcctaatct gacaggaacg gagaacttgg agatttttgc aaaactgcgt ggtacagccg
    13621 cccctaattc tgtaaaaaga gcattggaaa ttgtaggctt accttataaa aataaaaagc
    13681 tgtttagtaa atattcactt ggcatgaaac agcgacttgg tattgccaac gctatcctgc
    13741 atgacccaga actgttagtt ttagatgagc caaccaatgg tcttgaccct attggtattg
    13801 cggagatgag aaagtttatt agggacttga gcgttgagca tggaaagaca attcttattt
    13861 ccagtcatat tctttcagaa atctcattgt tggctgatga tattggaatt atcgaccacg
    13921 gtgttctttt ggaagaaagt agtatgagtg aactagaaaa gaaaaacagt aaatatatcc
    13981 tgctacaagt ttcagatatt ccgaagacat cacttatttt agagcgtcaa tttaacacga
    14041 aggattattc cgttcaagat gactacacgt tacaccttta taacactaat ctggatatgg
    14101 gtgaaatcaa taaagcactt gtaatgcaga atgtaactgt tattagttct caactttgta
    14161 atgatacttt agaggactac tttaaaaaaa tcacaggagg agagggcatt gcttaaactt
    14221 attcaaacag aatttcttaa actgcgtcgt aggaaactta tttggctgat gttgttgtct
    14281 gctctcatta tgccgttttt cgcactacta tacttcaatt attttggtaa aacaggtgtt
    14341 gagccaataa agttttataa atggtctgca tttggttata cactttggat tattttgcct
    14401 gttgttttgg gtatattttg caccatgctt atacacgaag aaaaccagta tgatatgctc
    14461 aaacagcttt ggattgtacc tattagtaaa atgggatact tttttagtaa atttttcgtt
    14521 gttttgattt actctatttg ttttatgctg attacagccg ttgcttctgt gttatttagt
    14581 gtactttctg gatatgtcgt atttagctgg gcaagcattt tatatttgtt gaaaaaatgt
    14641 ttagaaattg gtgtaatcac tgcttttgta atgttgccga ttttggcaat cgctacatct
    14701 caaaaaggat acattctccc aatttgtatc acattggtat atgccttttc cggttttgtt
    14761 ataatgacaa taaatatgta tttacaccca ttatccagta tgggagttat tatcatgaga
    14821 aataaagaca ttccaggtct tatattttca caggaaatta atgtcccatt agcatttctt
    14881 tgtatttgta tttggagtat tgtatcagta ttgtttgcaa atattgcact aggaagaaga
    14941 aagtgaggtg tagataatgt ggaaattact ttgggctgaa tgtcaaaaaa ttcgtcgttc
    15001 aaaaattgtt tggattacag tttttgcatc cattatgata gcagtcatag tttttgcagg
    15061 ggggcaggat gtatataatg acccagattt acattatggc ttaaagagcg tttatgacgg
    15121 ttcacggtat atagacaatg caggttggta tatggacgca gtacaacctt ggtcaacttt
    15181 ttttgttctt cctgctgtca ttgccctatt aggaagctat attatttgtc gtgaagaaga
    15241 ggaggacact attaaatccc tgcgtcttat tcccataaat gaaacaaatt taaccatagc
    15301 aaaaatgatt attactttta tattcagtat acttctttat ttgttactct ttactataac
    15361 atttttgact gaatccgttt tacatttttc cgatttatcg acaaaattgg ttttaagttg
    15421 catgaaagag tattttcttg atggcatagg tgtattcttt gcaatttctc ccattattgc
    15481 cttggtatcg cgcatgaaaa aaggatattg gcttgcatta gtattcacag aagtttattc
    15541 tattgctgga ctatttgcgg gtatgtcaaa tgtcttacaa accttttttc ctattgcggc
    15601 aatatttaat atttctggat actatataac tactggagaa aaaattatgg ggagtatcat
    15661 aagtttatta ctttgtgcct gcctttctgc atttatactt aaagggctaa aacacaatga
    15721 aaaaaattga aataggtata tcaaaatgaa aaagataaaa aaactgatta ttctaattgt
    15781 attcttgttt ctttttggca tgttacactg ttatactgaa tggaaattca acaataaccc
    15841 acctaaaggg gtaatagaaa gaataaatga gtggactgga ttagtaactt atgttaatta
    15901 tagtgaatgt aatattagca agttattgtt tcgtatttta atactataac tttaaatatt
    15961 tggttttaat cttccaaacg aatataaaaa aactgtcaca gaggcaacat atttttgaat
    16021 atttataaag tggtagtttt gattttcaaa gctaccgcct tttcttctat ataagcaaac
    16081 ttatcctcaa caaagtgtaa aacatggtac attttaagaa aaaagtacca cagacacaag
    16141 caataaaaga aacgccgaga acctacaaaa acgtagtgct tatgcagatt tacgacgcct
    16201 tctattttct caaccgtctt aatgaccttg tacaaaagaa atttttctaa tattttttat
    16261 aatcttatct tgtgatgttt tttatgtata aatgacttaa taacaaatcc aataaaaatt
    16321 atggatattg gtataattag aacgaggaat ttataatcat tataatgtga cattattctt
    16381 acccaactct cagaaaaaat atatcctata ctgattaatg aagtattcca tatggttatt
    16441 cccacgatag atgatattac aaaagttata atattttgcc ctgctatacc tgctataaaa
    16501 gatatgtatg ttctacataa agggattaat ctacatacac aaacagaaat agaagagtat
    16561 ttattaaaat aatcttgaga tgcaaatatt cctttctttg cctttggaca tatctctatt
    16621 attttattta tgatagattt acctccccaa tttcccactg tataacaaaa tatcgaacca
    16681 agtatacctg caattatact taaaattaaa attgtcagaa atccaaaatg atttcttgca
    16741 gcaattgctc cacataaagg taaaacaact tcgctaggta atggaaaaca agcatactca
    16801 atcataatta atacaaatac agagattaat ccatattgta aaataaaact attgattaca
    16861 gacacttact tatctatctc ctttcttatt taaaggtttt aagggtacat gttctaagga
    16921 ttctcaccct atatttaaag aatcaatctc tttaaactat tatatgtatt tacacaaagc
    16981 atttataaca tactttaaaa atagtacctt aaaataagaa acttaataaa taaaaaaaga
    17041 ttacgtggct acgtcctact ctcccaggca gtttcccacc aagtaccatc agcgctagag
    17101 agcttaactt ctgtgttcgg aatgggaaca ggtgtatcct ctctgctatt gtaaccacat
    17161 aatctttatg cttaatattt ccattaagtt tttaataagt ttatagaa
//
LOCUS       DAELLV010000061        16987 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.061, whole genome shotgun sequence.
ACCESSION   DAELLV010000061 DAELLV010000000
VERSION     DAELLV010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16987
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.061"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            26..337
                     /locus_tag="KPD58_001645"
     CDS             26..337
                     /locus_tag="KPD58_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBG0265418.1"
                     /translation="MYFTYIIRCKDDSLYTGYTSNIVRRMNEHKLGINSKYTRAKGFE
                     KLEVYFVTNTKSNAMKLEYYIKKLTRNKKLSIIKNPSILINLIDNKEDYIIGKEIEQL
                     T"
     regulatory      430..522
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            622..1416
                     /gene="thiD"
                     /locus_tag="KPD58_001646"
     CDS             622..1416
                     /gene="thiD"
                     /locus_tag="KPD58_001646"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBG0265419.1"
                     /translation="MSNYKIPTLTIAGSDSSGGAGIQADLKTFSAIGTYGMSVITAIT
                     AQNTKGVFAVEDLNKKIIKKQIEAVFEDIPPRAVKIGMVSSPEIILEIVENLKKYNPK
                     YLVVDPVMISKSGYYLLKPEAKENLIKYLIPLAYIITPNIPEAEEITGIKIHNVDDMK
                     RVGEEILQLGPKFVLMKGGHLDGEAVDILVGKNIFKVYKSERIDKKNTHGTGCTLSSA
                     ITSYLALGYEITEAVNLSKIYITEAIKRSFDIGHGVGPVHHFYKFE"
     gene            1440..2229
                     /gene="thiM"
                     /locus_tag="KPD58_001647"
                     /pseudo
     CDS             1440..2229
                     /gene="thiM"
                     /locus_tag="KPD58_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112777.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
     gene            2252..2884
                     /gene="thiE"
                     /locus_tag="KPD58_001648"
     CDS             2252..2884
                     /gene="thiE"
                     /locus_tag="KPD58_001648"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBG0265420.1"
                     /translation="MIDKESLKKCLKLYLVTDSEMLKGRDFYKCLEDAISSGITTVQL
                     REKNASGREFLRKAMKLREITKRYGVKFIINDRVDIALICDADGVHVGQSDIDVREVR
                     KLIGNNKILGVSARTLEEAICAKNDGADYLGVGSIFTTSTKLDAKSASFETVKEIKEK
                     VDMPFVLIGGINLDNIDKLKCLESDGYAIISAILKAEDISKEVEKWTLKI"
     gene            3023..3325
                     /locus_tag="KPD58_001649"
     CDS             3023..3325
                     /locus_tag="KPD58_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0265421.1"
                     /translation="MIRILLVCVGGMSSTLLVNKMEKDAKKRNIDCKIWAVGEGDIKS
                     ELDNFDILLLGPQLRFMLDDVKSIVGDRAPVSIIDMVNYGTCNGHAVLNSVLEILK"
     gene            complement(3391..4146)
                     /locus_tag="KPD58_001650"
     CDS             complement(3391..4146)
                     /locus_tag="KPD58_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0265422.1"
                     /translation="MGMLNILWRSMKWRFKNPISFVVTILQPFLWLVLYSSIANQTMN
                     NININNYTAFILPGIIVLVVFSSCSSGGIINFIMKNSGSFYRVLIAPISRYSIVLGQL
                     LEAILVSFIEVTILCIVSIFFSVRIESGIGGILLMIVLIFMTAFFLSSLAYSISLLLP
                     NEIVYETIMTAIVLPIFFLSSALFPIESLSGGLKVAVMLNPFTHVINALRSLIFGETI
                     LIGDIMPVILLFLILCCSSFSLAMWRLKKEMVS"
     gene            complement(4134..5096)
                     /locus_tag="KPD58_001651"
     CDS             complement(4134..5096)
                     /locus_tag="KPD58_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0265423.1"
                     /translation="MLAIEIDNLVKEYKNGVKALNALSFNVNAGEIFSLLGPNGAGKS
                     SLINILTTFYKPTSGNVTMFGKDLVDNPSWIRTQIACVAQQISIDEHLSLMENMVFQS
                     KMYKVEPQIAKQRIDSLIDKFDLSSYLKYPTSSYSGGVKRRLDIAMNMVSSPKILFLD
                     EPTVGMDVDSRKSMWDMLLKIRDEYGTTIFLTTHYLEEAEQLSDNICIMKNGKDLAQG
                     TPSSLRSYIRQNILRITFHNTEDIKKYKDAIKSTGLVKFMSVRENSIFISVNDSRTAF
                     TLINKWLLEHDIEFDAIEIVEPSLEDVFLALTSSKKSLKEEWEC"
     gene            complement(5100..5558)
                     /locus_tag="KPD58_001652"
     CDS             complement(5100..5558)
                     /locus_tag="KPD58_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBG0265424.1"
                     /translation="MNCKIEIKNIESVTVATMRYNGPMTEATKYFPNVFKAIKGKSNG
                     APFFCYYDVDQKTGVAEMELCVPTAETPNRMGITTKEFPKTKALFFTHTGSYSNFPKT
                     YEMIFKYIHENNIKIQTPWREVFIKGPGMLIKGNPDNYITEIIFPLKEEE"
     gene            5913..6269
                     /locus_tag="KPD58_001653"
     CDS             5913..6269
                     /locus_tag="KPD58_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0265425.1"
                     /translation="MNFELDNTTPIYLQIVKYIKRQIVTGELKPGETIPSRREMALNL
                     KVNLNTVQRAYKEMGDMNIINTFKNYQSSVTVDENILKNLKLELINESLSVFIEDMKA
                     INVSKEEVLKIIEDKY"
     gene            6341..7057
                     /locus_tag="KPD58_001654"
     CDS             6341..7057
                     /locus_tag="KPD58_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0265426.1"
                     /translation="MIEIKNVSKTYKRMQGLKIRKIEALKNVSFNIEKGKITALLGIN
                     GVGKSTMLKAIAGLINIDSGEIRIDGEKINEKVYNKLAFVPDVQSHFSNTTIKETFEF
                     MEIFYSKWNKEKSKEMMDIFKLDEDEIIDNLSKGNIARVKLILGFCQDPEYILLDEPF
                     TGIDLFKREEFIGVIAQYMEENQAIIITTHEIVEIESLVDEVVILDEGQIITSFNAEE
                     LREREGKSILDKMREVYKNE"
     gene            7050..7874
                     /locus_tag="KPD58_001655"
     CDS             7050..7874
                     /locus_tag="KPD58_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0265427.1"
                     /translation="MNKLLYLINVDLRRSNKFYIAYISFFSLITLSLNLFEINKFKNS
                     KFIMNLAIEDFGGIFYGLGILSNLGFIQSILFFGILGLFIYYIFMWKREFFSNNQSIY
                     TLMMLPQNKFKICISKSIALITMIYGFLISQTATLFICKYIFNFIFRNMPIINMNFAK
                     DLYYLNLKSIPIDFISFMAIYVFLLLIAISIVNCCILFIFSYINKFYVILITLLIIAG
                     LYFSTIYINIITLFRKYYSLIGFQFNNLVAGLLSFLIIMFILNGISYLLIKKKVSL"
     gene            7886..9085
                     /locus_tag="KPD58_001656"
     CDS             7886..9085
                     /locus_tag="KPD58_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265428.1"
                     /translation="MKLKSKISILIIFLTLAIYFSIGLLNNEDNLKIKTIKGDSNELD
                     HININFGYALNGYINSYYTINGSNKTKKTELIKLQKDSKNTNNLIDVSSGSDLIAHVG
                     FEMNEESKDINFVIDEKKEDLEKGVFPSEGSDKEIEYEKLDMNNFTRTRYKVDKSTDD
                     YYANILASKRYKKYLYVSVLYTTSDSSENNKEYSSTIDIIKINPENKKIKTIKSINLS
                     NYFSEKYQNIETIGVSYASKYEDKMYILVNVLNTNIGDNSNSKFHLLEYNLEENKESI
                     MELKTDSKMALAKAKLEANKLYMVMSNDYDNSSITELNLLTYNILDKSLDEDKYVLDN
                     REKRGIRSIEIDSNKIVAYTSRLNSILSWDNKFMLYIINKNSKEIVYEGELQFNSSNS
                     MLEFEIK"
     gene            9094..9906
                     /locus_tag="KPD58_001657"
     CDS             9094..9906
                     /locus_tag="KPD58_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265429.1"
                     /translation="MKDLIRLFEFELKRNIKNYIFIIICCCSFVILNIVKNLNDYNYI
                     IENAVKSEQLTKIGNTIETVKVTGFSSFRNIMGSTESWFMFGIIACICYAFFIWYRDF
                     NGRSKSIYTLIMLPKNRINIYISKLLNILFLVYSYTVVLTISLFIASKLLPRHMLGNV
                     TNYGFVQETIYELKMLLPYSFEILFVEYIFLLIGFVSVVFTSILINKSIYKVSTLISF
                     LFLVIEILVFIISIEFIIPYEYSDVFTVLYSSINIFVCSLISNKLLKQKIDF"
     gene            9917..11185
                     /locus_tag="KPD58_001658"
     CDS             9917..11185
                     /locus_tag="KPD58_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265430.1"
                     /translation="MKAKNIGKYTICFIIVCFLMITMTLILSYFNKDKLSVKTLKGNV
                     KDIGRVSVVYTPNISKFSYEEVIISKNGVRNNTNPKSRIRNDYLNIDKKYSDFVNDKG
                     KEFIYKDDLNIGEAYTSVKSDLVQGMNALNDCIKVSNKNLKSGVEENFDINLNKSDFK
                     ENSWKTLFNKIYKGNLYMVISETNISGSKVNNICILKIDIKNKSYKLLNRFNLGLQNG
                     EQQNINEIKFSIGSKLYLEIVNNKSKNGYIEGHDSYSNFIIYDIEDNTFNISNKFMDN
                     LNKEKNANPKEIGLLSKTKLDYMVKNNKLNLIVTDMSNSITNLVYDINSNEIKLEDYE
                     ILDLKVDYNEASSGVRKVKLLDDKIYSISGIKNSISGELKGFKGIVDVVGTKPVKFQV
                     FDMKLKKNIYEAELINGDISLQEKLFFVAN"
     gene            11551..12732
                     /locus_tag="KPD58_001659"
     CDS             11551..12732
                     /locus_tag="KPD58_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG0265431.1"
                     /translation="MNKKYIKAGNLIDGTGNSIMQNKGIIIDGDTIVEIEDIRDDLNG
                     YDVYDYLDKTVMPGIMNCHVHLTMEPVGNPKIYYDNVSDVELVVNTIKQLDAYLDSGV
                     TYIRSLGCPKYVDVQLKNLIDKGSIDGPGIVTSGPVICMTGGHGYYFGIESDGVDECR
                     KSARTVLKNGVDCVKIMATGGVTTDGVEPGSPQLTLEEMKAAVDEAIKAGKITATHAQ
                     GRTGIKNAVLAGITSIEHGVYLDDEIIDLMLERGTYLVPTVAAPYFILNAGKESGITE
                     ATLRKCEIVAIPHKESFLKAYKKGIKIAVGSDAGTSYNEHGKTYYEMKLMSDYGMDNM
                     DIIVAATKTASELLRIDKNYGTLEKGKKADVIVVNGNPVENIDVLADVLAVFKLGKKV
                     R"
     gene            complement(12874..13791)
                     /locus_tag="KPD58_001660"
     CDS             complement(12874..13791)
                     /locus_tag="KPD58_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265432.1"
                     /translation="MENNKCREDFRFTQEYEEDYPNTNERYYENYQVADRYYNYPNKY
                     KEPKIKQCCCKKSMREALELLRYDALRPFVNFNQFAFISDFFIVGANLVGIDLSAPPK
                     DNLSGLDGTFERFSACNCDLIDIAGRVSYPIPVPLTLEGLINTIGTIPGVAELIALID
                     AVIPPTIDLGAILDAILAAIIDFILAASTPLANVDLASLCNLKAVAFDITPADYEDFI
                     ASLGYYLDKKHYKECNCNCDCDDCCCNKGILDNLYMSNINNQVTVVAGSLVLTGVEVL
                     GKKNDVIVLGNSNDSRIYFVCVDSIDYIA"
     gene            14044..14838
                     /locus_tag="KPD58_001661"
     CDS             14044..14838
                     /locus_tag="KPD58_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0265433.1"
                     /translation="MIEVDKLCKSFTRVVKDDKNKSSIRKLKKVKTKKEEFFAVNNVS
                     FKVMEGEIVGILGPNGAGKTTLLRMLGGILTPTSGSINISGYDYSIDRNSAKKEIGYL
                     SGNTKLYGRLSPRELLTTFASLYEMSKEDIEESIENIVKIMDMSEFIDNRIENLSTGQ
                     TQRTSIARCLIHSPKVYIFDEPTLGLDVLSSASIIDFMKNEKLRGKTVLYSTHYMEEA
                     ETLCDKIFMIHNGKIIASGTPQSLKEESGVNNLRDVFIKLARKEDI"
     gene            14839..16899
                     /locus_tag="KPD58_001662"
     CDS             14839..16899
                     /locus_tag="KPD58_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG0265434.1"
                     /translation="MRSKIVKYIFKKEMLDILRDKKTLFMMIVVPLLLYPLLMLLMIQ
                     VMNMSASSMNSKDINIAFNNKPNKVLVSMIEDDNLEKDTKNTSNDKGKIRVLDVDNYK
                     KSLEENKIDAYIKMEEKKSSINYQIYINSSQENSSNALKRIETVLDKYKRFTIEKTLS
                     EKGLDVQATLEPITYSTVDVAKTEEVAGYFLGQILPFILIIGVLLGSIYPAIDVMAGE
                     KERGTLETLFTLPISNLELVMGKYMAVSLSAIVTAILNILSMGLTMAFILVSGGISSQ
                     FGFGNFNYGDLVLPIITTLICICLFSMVVSAISMCVCSLAKSFKDAQNYITPLMLIIM
                     IPSYVSMIPNIVLDRVTAVIPVVNISLLIKSVLSFKSDLNLIALVLASNVAFVILAII
                     LLSKMFNSEEILFGNSKSFSFLEKRSNIKKGSIPTVSDGVILYAVGLVLLIYVGSLVQ
                     IKFGMAGIIMTQFMIISLPLLFAYYIKADFKEVFSLKVPKLKHVFGSIVLWIGGYILI
                     MIITQLILFLFPQNMEVAESLNKALFIKDSVLLNLLAIALLPALCEEMFFRGFIFSSF
                     SKSKDKNKSIKLAIICSGVLFGIMHMDFIRIIPTSILGIIFAYSVYKSGSIFVSMLLH
                     FLNNSVAVVLNHYTTGNITNLYKFVEVDFSNLNVLKFALILLISLILIMLGLRVLDRK
                     SLRN"
BASE COUNT         6707 a         1816 c         2722 g         5742 t
ORIGIN      
        1 atttaatgtt aggatgtgat ttaaggtgta ttttacatat attataagat gtaaagatga
       61 ttctctatac actggatata ccagtaatat agttagaaga atgaatgaac ataaattagg
      121 aattaattca aaatacacaa gagcaaaggg atttgaaaaa ctagaagttt attttgtgac
      181 caacacaaaa agcaatgcta tgaagttaga atactatata aaaaaattaa ctagaaataa
      241 aaaattgtct ataataaaaa acccaagtat acttataaat ttaattgata ataaagaaga
      301 ttatattatt ggtaaggaaa tagaacaatt aacataaata tttatagtaa cattataatg
      361 ggtgcatatg gtgtaaaaac ttgacaataa atactattgt aatataatga agttataaat
      421 atagtaaatt aaaatagcta ggggagccaa gagctgagag gtataccgac ccttacacct
      481 gatctggata ataccagcgt aggaaagctt agtaaaaatt caagtatgta ttatttgtga
      541 tatatgctat ttctaagcta tacctattgt aggtatagct tttttaatta agttatttaa
      601 attaaacttt aggaggcaac aatgagtaat tataaaatac caacacttac aatagccggc
      661 tctgattcat ctggaggagc tgggattcag gctgatttga aaacgtttag tgcaattggt
      721 acttatggta tgagtgtaat aacagctata acagcacaga atactaaagg agtttttgct
      781 gtagaggatt taaataagaa aataataaaa aaacaaatag aggcagtctt tgaagatata
      841 cctccaagag ctgtaaaaat aggaatggtt tcaagtcctg agattatact tgaaatagtt
      901 gagaatctaa agaaatacaa tccaaaatat ttggttgtgg acccagtaat gatatctaaa
      961 agtggatatt atctattaaa gccagaggca aaagaaaact taattaagta tttgatacca
     1021 cttgcataca taattacacc aaatatacca gaggcagaag aaataacagg aataaaaata
     1081 cataatgtag atgatatgaa gagagtagga gaagaaatac tacaacttgg acctaaattt
     1141 gtacttatga aaggaggtca tttagatgga gaagcagtgg atattttagt tggtaaaaat
     1201 atatttaaag tttataaaag tgaaagaatt gataaaaaaa atacacatgg aacagggtgc
     1261 acattatctt ctgcaataac atcttatcta gcattaggat atgaaataac agaagcagta
     1321 aatctaagta aaatctatat aacagaagca ataaaaagaa gttttgatat aggtcatgga
     1381 gtaggaccag tacatcattt ttataaattt gagtaataaa aaaatattgg aggtagaata
     1441 tgtataattt aattaaagat gtaaaaaaat taaatccatt ggtaattcat tatactaata
     1501 atgtaactat aaatgattgt gctaatgtaa ctttagctgt aggagcaagt cctcttatga
     1561 gtttttcata tgaggaagta gaagaaatgg taagtgtagc aaactctgta gtaataaata
     1621 taggaactta tgaactcaaa tatgttggat ttattcctat tagctggaaa agcagcaaat
     1681 aagtacaata aacctgttgt tttggaccca gttggagtat ttgcaagcaa agcgagagca
     1741 gaacttacaa gtagactttt aaatgaagtc aaatttagtg ttgttaaagg gaatgtatca
     1801 gaaataaagt ttataggtgg ctttaatgta aagggtaaag gtgtagattc atttgatgaa
     1861 gaagaagatt ctactgagat aataagaaag attgctgaaa agcttgaatg tgtagttgta
     1921 gcaactggaa aaatagatat aataactaat ggtaaaggta catataaaat aaacaatgga
     1981 actgataaat taaaaggaat tacaggaact ggatgtatga ctgctagctt gattgcaagt
     2041 tttatggctg ttactgagaa tatattagaa gcagcaacaa tgggtgttct tacaatgtct
     2101 ttaagtgggg aattagctaa tttaaataat ccaccaatag gtacatttaa agaaaatctt
     2161 atgaatgcta tttatcaaat ggatatagat actttaagta aaaattctaa tattgaattt
     2221 ttaaattaat aaagagtaag ggagacttaa aatgattgat aaagaatctt taaaaaaatg
     2281 tttaaagttg tatttagtaa ctgactctga aatgcttaaa ggtagagact tttataaatg
     2341 tcttgaagat gcaatatctt ctggaataac aactgtacaa cttagagaaa aaaatgcttc
     2401 tggaagagag tttttaagaa aagctatgaa acttagagaa ataactaaaa gatatggtgt
     2461 taagtttata ataaatgata gagtggatat agcacttatt tgtgatgcag atggagtaca
     2521 tgtaggacag agtgacattg atgttagaga ggttagaaag cttataggaa acaataaaat
     2581 tctaggagta tcagcaagaa ctttagaaga agctatttgt gcaaaaaatg atggcgcaga
     2641 ctatttaggt gtagggtcta tattcacaac atctactaaa ttagatgcaa aatcagcttc
     2701 gtttgaaact gttaaagaaa ttaaagaaaa agtagacatg ccatttgtac taattggagg
     2761 tataaattta gacaatatag ataaacttaa atgtttagaa agtgacgggt atgctataat
     2821 atcagctata ttaaaagctg aagatatctc taaagaagtg gaaaagtgga cattaaaaat
     2881 ataaaattgg ctctggtagt gaaaaaaatg gaaaaatttg atataatcta agagtaaaat
     2941 ttaagcaatt ataaatgata aacttttcta catatacttt ttaatcaaga tatatgtaga
     3001 tttggttaaa aaggagctat aaatgattag gatattgtta gtgtgtgttg gaggcatgtc
     3061 ttctacccta ttggtaaata aaatggagaa ggatgcaaaa aaaagaaata tagactgtaa
     3121 aatatgggca gttggagaag gagatataaa atcggagctt gataactttg atattttact
     3181 tcttggacca caacttagat ttatgcttga tgacgtaaag tctatagttg gagatagagc
     3241 tccagtatct ataatagata tggtaaacta tggcacttgt aatggtcatg ctgtgttaaa
     3301 ttcggtttta gaaatattaa aataaataat aaaaatggat tctatataat agagtccatt
     3361 tttattattt gttattaata ggcttaaaac ttagcttacc atttcttttt ttaatctcca
     3421 catagcaagt gaaaaacttg aacagcataa tattaaaaat agtaggataa caggcattat
     3481 atctccaata agaatagtct caccaaatat taagctacgt aaagcgttaa taacatgggt
     3541 aaatggattg agcatcactg ctacttttaa tcctccagat aaactttcta tagggaaaag
     3601 agctgaactt aaaaagaaaa taggaagtac aattgcagtc ataattgttt cataaactat
     3661 ttcatttggc aataataaac ttatagaata agctagactt gataaaaaga aggctgtcat
     3721 aaatatcagt actatcataa gtaggattcc accaatgcca gattctatcc ttactgagaa
     3781 gaatatactt actatacaca aaatagttac ttctatgaaa gaaactaaga tagcttctaa
     3841 taattgacca aggacgattg aatacctact gataggtgca attaagactc tatagaaact
     3901 tccactgttc ttcattataa agtttataat accaccacta ctacaagaag aaaacacaac
     3961 cagtacaatg atacctggta gtataaaagc agtataatta ttgatattta tattattcat
     4021 agtttggttt gcaatagaac tataaagcac aagccaaaga aaaggttgta atatagtaac
     4081 tacaaatgag attggatttt taaaacgcca cttcatacta cgccataaga tatttaacat
     4141 tcccattcct cctttaagct ttttttagaa cttgtaagtg ctaaaaatac atcttcgaga
     4201 cttggctcta caatttctat tgcatcaaat tctatatcat gttctaaaag ccatttattt
     4261 attaaagtaa aagcagttct actatcgttt acacttataa aaattgaatt ttctcgtacg
     4321 ctcataaact ttactaaacc agtactttta attgcatctt tatatttttt tatatcttca
     4381 gtattatgga aagttatacg tagtatattt tgtctaatgt aactacgtaa acttgatggt
     4441 gttccttgtg ctaaatcctt tccattcttc ataatacaaa tattatcact caattgttca
     4501 gcttcttcaa gataatgagt agttagaaat atagttgtgc catactcatc tctaattttt
     4561 aataacatat cccacataga ttttcttgaa tcgacatcca taccaacagt tggttcatct
     4621 aaaaatagga ttttaggaga tgataccata ttcatagcta tgtccaatct acgttttaca
     4681 ccccctgaat atgatgaagt aggatatttt aagtaactag ataagtcaaa cttatcaatt
     4741 aaagagtcta ttctttgttt tgcaatctgt ggttctactt tatacatttt actttgaaat
     4801 accatatttt ccataaggga taaatgttca tcaatagaga tttgctgtgc tacacaagca
     4861 atttgagtac gtatccaaga aggattatct actaaatctt ttccaaacat tgttacattt
     4921 ccagatgtag gtttgtaaaa ggtagtcaaa atatttataa gagaagattt accagcacca
     4981 ttaggaccaa gtaaagaaaa aatttctcca gcatttacat taaagcttag agcatttaaa
     5041 gctttcacac catttttata ttcttttact aaattgtcta tttcaatcgc taacatatat
     5101 tattcctcct ctttaagtgg aaatattatc tctgtaatat agttatctgg gtttcctttt
     5161 atcaacatac ctggtccttt aataaatact tctctccaag gagtttgtat ttttatatta
     5221 ttttcatgaa tatatttaaa tatcatttca tacgtttttg gaaaattact ataagagcct
     5281 gtgtgagtaa aaaataaagc ttttgtttta ggaaattctt ttgtagttat acccatacga
     5341 tttggagttt cagcagttgg aacacataat tccatttctg caacaccagt tttttggtca
     5401 acatcatagt agcaaaagaa tggtgcaccg ttagactttc cttttatagc tttaaataca
     5461 tttggaaaat actttgttgc ctcagtcata ggtccattat aacgcatggt agccactgtg
     5521 actgattcga tatttttgat ttcaattttg caattcattg tttacctcct aatgtgtgtt
     5581 catattctta ctagattttt aagcattaat acatataaga ttttgattag ttgaaaacat
     5641 ataatcttat ctcgtttgct tgttttcaag tctaacataa cataaaatat atatcctctc
     5701 atttgatgct ccattttgta aagttattat ttaagcttta ctttagattt tttagcaaat
     5761 taaaataaag caatttataa aataagtatt tacaaattaa gtgtattaat cgtataatac
     5821 acttaatagg atactagata aagtctgaaa taaaggcgta tttttttaaa gtaagtgtac
     5881 tatcgtatta atacactaga ataggaggaa tcatgaattt tgaattagat aatacaactc
     5941 ccatttattt gcaaatagta aagtatataa agagacaaat agtaacagga gagttgaaac
     6001 caggagagac aataccatca agaagagaaa tggcacttaa tctaaaagta aacttaaata
     6061 cagttcaaag agcatataag gagatgggtg atatgaatat aataaatact tttaaaaatt
     6121 atcaaagcag tgtaacagtt gatgaaaata tattaaaaaa tttaaaatta gagcttataa
     6181 atgaatcact atctgttttt atagaagata tgaaagcaat aaatgttagc aaagaagaag
     6241 ttttaaaaat aatagaagat aagtattagt tttttaacaa atagaaaact tagtttagaa
     6301 agtttattta taagcatatt ggatgtggag gatagtaaat atgatagaaa taaagaatgt
     6361 cagcaaaaca tataaaagga tgcaagggct taaaataaga aagatagaag ctttaaaaaa
     6421 tgtaagtttt aatatagaaa agggaaaaat aactgcatta cttggtataa atggtgttgg
     6481 taaatcaact atgttaaaag caatagcagg acttataaac attgatagtg gagaaattcg
     6541 tatagatgga gaaaaaataa atgaaaaggt atacaataaa ttggcttttg ttccagatgt
     6601 acaatctcac tttagcaata caacaataaa agaaacattt gaatttatgg aaatttttta
     6661 ttcaaagtgg aataaagaaa aatcaaaaga aatgatggac atatttaaat tggatgaaga
     6721 tgaaataatt gataacttgt caaaaggaaa cattgcaaga gtaaaattaa tacttggatt
     6781 ttgccaagac ccagagtaca tacttcttga tgaaccattt acaggaattg atttgtttaa
     6841 gagggaagag tttataggag taatagcgca atatatggag gaaaatcaag ccattatcat
     6901 aactactcat gagatagtag agatagaaag tttagttgat gaggttgtaa ttcttgatga
     6961 agggcagatt ataacttcat ttaatgcaga agaattaaga gaaagagaag gtaaatctat
     7021 attagataag atgagggagg tatataagaa tgaataaact tctatatcta ataaatgtag
     7081 atttgagaag aagcaataag ttttatatag cttatatatc atttttttca ctaattacac
     7141 ttagtttaaa tttattcgaa attaataaat ttaaaaattc taaatttatt atgaacttag
     7201 caatagaaga ttttggaggt atattttatg gactagggat acttagtaat cttggtttta
     7261 tccaaagtat attgttcttt gggatattgg gactttttat atactatatt tttatgtgga
     7321 aaagagagtt cttttcaaat aatcaaagta tttatacact tatgatgtta cctcaaaata
     7381 agtttaagat ttgtatatct aaatcaatag ctttgataac aatgatatat ggttttttga
     7441 taagtcagac agcaacatta tttatatgta aatatatatt taatttcatt tttagaaata
     7501 tgcctataat caatatgaac tttgccaaag atttatatta tttaaacttg aagagtatac
     7561 caattgattt tatctctttt atggctatct atgtattttt actacttata gctatttcta
     7621 ttgtaaattg ctgtatatta tttatctttt cctatataaa taaattttat gtaattttaa
     7681 tcactttgct gattatagca ggcttatatt tttcaacaat atatataaat ataatcactt
     7741 tatttagaaa atattatagt cttataggat ttcaatttaa caatttagta gcagggttat
     7801 taagtttttt aattataatg tttattctaa atggaatatc ttatttgcta attaaaaaga
     7861 aagtatctct ttaaggaggg gtggtatgaa attaaaatct aaaatatcta tacttattat
     7921 atttttgact ttagcaatat attttagtat aggtttatta aataatgaag ataatttaaa
     7981 gataaaaact ataaaaggtg actctaatga attagaccat ataaacataa attttggata
     8041 tgctttgaat ggatatataa attcatatta tacaataaat ggaagtaaca aaaccaaaaa
     8101 gactgaatta ataaaactac aaaaggatag taagaacaca aataatttaa ttgatgtatc
     8161 tagtggttca gatttaattg cacatgttgg atttgaaatg aatgaggagt caaaagatat
     8221 aaactttgta atagatgaga aaaaagaaga tttagaaaaa ggtgtatttc catcagaagg
     8281 aagtgataaa gaaattgaat atgaaaaatt agatatgaat aattttacaa ggacaagata
     8341 taaagtagat aaaagtacag atgattatta tgcaaatata ctagcatcaa aaagatataa
     8401 aaaatactta tatgtatctg tactttatac tacaagtgat tcatcagaaa ataacaaaga
     8461 atattcaagt actatagata taataaaaat taatccagaa aataaaaaaa tcaagactat
     8521 aaaaagtata aatttaagta attatttttc cgaaaaatac caaaacatag agaccatagg
     8581 tgtaagttat gctagcaaat atgaggataa gatgtatatt ttagtaaatg tactaaatac
     8641 aaacataggt gataatagta attctaaatt tcatttactt gagtataatc ttgaagaaaa
     8701 taaggaaagc ataatggaat taaaaactga tagcaaaatg gctttagcaa aagcaaagtt
     8761 agaagctaat aaattatata tggtaatgtc gaatgattat gataatagtt caattacaga
     8821 gttaaattta cttacctata atatattaga taaaagctta gatgaagata aatatgtttt
     8881 agataataga gaaaaaagag gaattagaag tattgaaatt gattcaaata aaatcgttgc
     8941 atatacatca aggctaaact ctatattatc ttgggataat aaatttatgc tatatataat
     9001 aaataaaaat tctaaggaaa ttgtatatga aggagaatta caatttaaca gttcaaatag
     9061 tatgttagaa tttgaaataa agtaggtgag ttgatgaaag atttaataag attattcgaa
     9121 tttgaactaa aaagaaatat aaagaattat atatttataa taatttgttg ttgttctttt
     9181 gtaattttaa acatagtaaa aaatttaaat gactataatt acataataga aaatgcagtt
     9241 aaaagtgaac agttaacaaa aatcggaaat acgatagaaa cggtaaaggt tacaggtttc
     9301 tcaagcttta gaaatataat gggttctaca gaatcgtggt ttatgtttgg tataattgca
     9361 tgtatttgct atgctttttt tatttggtat agagacttta atggtagaag taaatctata
     9421 tatacactta taatgctacc taaaaataga atcaatattt atatatctaa attattaaat
     9481 atattatttc tagtgtatag ctatacagta gtacttacaa tatctttgtt tattgcaagt
     9541 aagttgttgc ctagacatat gcttggtaat gtcacaaact atggttttgt acaagaaaca
     9601 atatatgagt taaaaatgtt acttccatat agctttgaaa tactatttgt agagtatata
     9661 tttttattga taggatttgt atctgtagta tttacatcaa ttcttataaa taaatctatt
     9721 tataaagtaa gcactttaat aagttttcta tttttagtta tagaaatttt agtgtttata
     9781 atttctatag aatttattat accctacgaa tacagtgatg tatttactgt tctatattca
     9841 agtataaaca tatttgtatg tagtcttata tctaataaac ttttgaagca aaagattgac
     9901 ttttaggagg gatattatga aagcaaaaaa tataggaaaa tatacaatat gttttataat
     9961 agtatgtttt ttaatgatta ctatgacctt aatattgagt tattttaata aggataaact
    10021 atctgtaaaa actttaaaag gcaatgtaaa agatataggg agagtaagtg tagtatatac
    10081 acctaatata agcaaatttt cttatgagga ggtaattata agtaaaaatg gagtaagaaa
    10141 taatacaaac ccaaaatcaa gaattagaaa tgattattta aatattgaca aaaaatacag
    10201 tgactttgtt aatgataaag gaaaagaatt tatatataaa gatgatttga atataggaga
    10261 agcgtatacc tctgttaagt ctgatttagt acaaggaatg aatgctttaa atgattgtat
    10321 aaaggtttca aataaaaatt taaaaagtgg ggtagaagaa aattttgata ttaatttaaa
    10381 taagtctgat tttaaagaaa atagttggaa aaccttattt aacaaaatat ataaaggtaa
    10441 tttatatatg gtaattagtg aaactaatat ttcaggttct aaagtcaata atatttgtat
    10501 actaaagata gatataaaaa ataagtctta taaattatta aatagattta atttaggatt
    10561 acagaatgga gaacaacaaa atatcaatga aattaaattt tctattggaa gtaaattata
    10621 cttagaaata gtaaataata aaagtaaaaa tggatatatt gaaggacatg atagttactc
    10681 taactttatt atatatgata tagaagataa cacttttaat ataagtaata aatttatgga
    10741 taatctaaac aaagaaaaga atgcaaatcc aaaggagatt ggcttattaa gtaaaactaa
    10801 gctagattat atggtaaaaa ataataaatt gaatcttata gttacggata tgagtaattc
    10861 cataactaat cttgtttatg atattaattc taatgagatt aaattagaag attatgagat
    10921 acttgattta aaagtagatt ataatgaagc atcatctgga gttaggaaag ttaagctact
    10981 ggatgataag atatattcta tttcaggtat taagaacagt atatcaggag aattaaaagg
    11041 atttaaaggg attgttgatg tagtcggaac taagccagta aaatttcaag tatttgatat
    11101 gaaacttaaa aagaatattt atgaagctga gctaataaat ggagatataa gtttacaaga
    11161 aaagttattt tttgttgcaa attaagtgtt agaattaatt ttagaacttt aaacttttat
    11221 atgtatcaca gataagtata agtaagggag tgtctcaaaa taaacttttt agctcatgag
    11281 acactttttt tatatgaaat caaatatatt ttcattgttt agatttattt tgataatgaa
    11341 aaaaagatta tctttatttt aagaaagaca tttatttata attatataca aaaatatttt
    11401 ttaatgtttt tgtgtataat tataacaatt taatttatat taatgcactt aaatttgaaa
    11461 tagaatgctt atagtggtaa aattaattat tagaatatta gaaataataa ataaataata
    11521 cattgtactt aaaaggggga gaaatttaag atgaacaaaa aatatattaa agcagggaat
    11581 ttaattgatg gaaccggaaa tagtattatg caaaataagg gtataattat tgatggagat
    11641 actatagttg aaattgaaga tataagagat gatttaaatg gatatgatgt gtacgattac
    11701 ttagataaaa ctgttatgcc aggcattatg aattgtcatg ttcacttgac aatggagcca
    11761 gttggaaatc caaagatata ttatgataat gtatcagatg tagagcttgt agtaaatacg
    11821 ataaaacaac ttgatgctta tttggattca ggagtaacat atattagaag cttaggttgt
    11881 cccaaatatg tggatgtaca acttaaaaat cttatagaca aagggtctat agatggtcct
    11941 ggtatagtta cttcaggtcc agttatatgt atgacaggag ggcatggata ttattttggt
    12001 attgaaagtg atggagtaga tgaatgtaga aaatcagcca gaacagtttt aaaaaatgga
    12061 gtagattgtg taaagataat ggcaactgga ggtgtaacaa ctgatggagt tgagccaggg
    12121 tcaccacaat taactttgga ggaaatgaag gctgctgtag atgaagctat aaaagcaggt
    12181 aaaataactg ccacacatgc acaaggaaga actggtataa aaaatgcagt gcttgcagga
    12241 attacttcta tagaacatgg tgtttattta gatgatgaaa taattgattt gatgcttgaa
    12301 agaggaactt acttagttcc aactgtggct gcaccatatt ttattctaaa tgcaggaaaa
    12361 gaaagtggaa taactgaagc tactcttaga aagtgtgaga ttgttgccat tccacataaa
    12421 gagtcattcc taaaagcata taaaaaagga attaaaattg cagttggttc tgatgcagga
    12481 acttcttata atgaacatgg aaaaacatat tatgaaatga aactaatgtc agattatggt
    12541 atggataata tggatataat agttgctgct acaaaaacag catctgaact tttaagaatt
    12601 gataaaaatt atggaactct agaaaaagga aagaaagcag atgttatagt tgtaaatgga
    12661 aatccagtag agaatataga cgtacttgca gatgtgttag cagtatttaa acttggtaaa
    12721 aaagttagat aaatagtata aaaataaaaa agagtgtcta aagaaatttt taaataatta
    12781 tagacactct tttttattag taataattaa atcttttata ctttagaaaa tagattctat
    12841 tttatattta gatataactc tttaaaatca ttcttatgca atataatcta tagaatctac
    12901 acatacaaag tatattcttg aatcattaga atttccaagt actataacat catttttctt
    12961 acctagaact tcaacaccag ttagaaccaa actaccagct actacagtaa cttgattatt
    13021 tatatttgac atataaagat tatctaggat acctttatta caacagcaat catcacaatc
    13081 gcagttgcaa ttacattctt tgtaatgttt tttatcaaga tagtaaccta aagatgctat
    13141 gaaatcttca taatctgcag gtgtaatatc aaatgcaaca gctttaagat tacacaatga
    13201 tgctaaatct acgtttgcta atggagtaga tgcagcaagt ataaaatcaa ttatagcagc
    13261 aagtattgca tctaatatag ccccaaggtc tatcgtagga ggaataactg catcaataag
    13321 tgcaattaat tcagctactc ctggtatagt tcctatagta ttaattaatc cctcaagagt
    13381 taaagggact ggaataggat aagatactct accagctata tctattaaat cacagttaca
    13441 agcagaaaat ctttcaaaag taccatcaag tccagataaa ttatcttttg gaggagctga
    13501 aagatctata cctaccaaat tagcacctac tataaagaaa tctgagataa aagcaaattg
    13561 attaaagttt acaaaaggtc ttagagcatc atatcttaga agttctaatg cttctctcat
    13621 acttttttta caacaacatt gttttatttt aggttcttta tatttatttg gataattata
    13681 gtatctatca gctacttgat aattttcata gtatctttca tttgtatttg gataatcttc
    13741 ctcatattct tgtgtaaatc taaagtcctc tctacattta ttattttcca ctaaatacac
    13801 tccttttata ttttaattta aatgtataga taattaagct tatctatata gtaatattat
    13861 atttataatg taaaaaaatg ttacaaaaga tattataaac gaataaaaaa taataatttg
    13921 ccagataaac ttgtaaattt ttatgaataa tccattctaa aacaaaaaac tgtgtataaa
    13981 aaaggtttat atgttatatt tatataaata taacatatga acgagaaagg agtggtttga
    14041 taaatgattg aggtagataa attgtgtaag tcttttacaa gggttgtaaa agatgataag
    14101 aataaaagtt caataagaaa gctaaagaaa gtaaaaacta aaaaagaaga attttttgct
    14161 gtaaataatg tttcatttaa agttatggaa ggtgaaattg ttggaatatt aggtccaaat
    14221 ggtgcaggaa agactacatt acttagaatg ttaggtggca tattaacacc aacatcagga
    14281 agtataaata tttctggata tgattattca atagatagaa attcagcaaa aaaagaaata
    14341 ggttatctct ctggaaatac aaaactatat ggaaggcttt ctccgagaga acttctaaca
    14401 acttttgcaa gtctatatga gatgtcaaaa gaagatatag aagaatcaat tgaaaatatt
    14461 gtaaaaataa tggatatgag tgaatttata gataatagaa ttgaaaatct atcaactggt
    14521 cagactcaaa gaacatctat agctcgttgt ttaatacatt ctccaaaagt atatattttt
    14581 gatgagccaa cactaggtct ggatgtttta agtagtgcat ctattataga ttttatgaag
    14641 aatgaaaaac taaggggaaa aactgttctt tactcaactc attatatgga agaagcagaa
    14701 actttatgtg acaagatttt tatgattcac aatgggaaaa taattgcatc aggaacacca
    14761 cagagtttaa aagaggaatc tggagtaaat aatttgagag atgtattcat aaaacttgcc
    14821 agaaaggagg acatttaaat gagaagtaag attgtaaaat acatatttaa aaaagaaatg
    14881 ttagacatat tgagagataa aaagacttta tttatgatga ttgttgtgcc acttctttta
    14941 tatcctttac ttatgttatt aatgattcaa gtaatgaata tgtctgcaag tagtatgaat
    15001 agtaaggaca ttaatatagc ttttaataat aaacctaata aggttttagt atccatgatt
    15061 gaagatgata atttagaaaa agatactaaa aatacttcaa atgataaagg caaaataaga
    15121 gtgttagatg tagataacta taaaaagtca ttagaagaaa ataaaattga tgcttatata
    15181 aagatggaag aaaaaaaatc atctataaat tatcaaatat atataaattc ttctcaagaa
    15241 aattcttcta atgcactaaa aagaattgaa actgttttgg ataaatataa aagatttacc
    15301 atagaaaaaa ctttatctga aaaaggatta gatgtacaag ctactttaga accaataaca
    15361 tatagcactg tagatgttgc aaagacggaa gaagtggcag gatacttttt aggtcagata
    15421 cttccattta tattaataat aggagtttta ctaggttcaa tatatccagc aatagatgtt
    15481 atggctggag aaaaggaaag aggtacatta gaaacattat ttacactgcc aatatcaaac
    15541 ttagaactgg ttatgggaaa atatatggca gtttctctta gtgcaatagt aacagcaatt
    15601 ttaaacattt tatctatggg actgactatg gcatttattt tagttagtgg aggtattagt
    15661 agccaatttg gctttggaaa ctttaattat ggagacttag tcttaccaat tataactact
    15721 ttaatttgca tatgtctttt ttcaatggta gtttcagcaa taagtatgtg tgtatgttca
    15781 ttagcaaaaa gttttaaaga tgctcaaaac tacataacac ctcttatgtt aattattatg
    15841 ataccatcat atgtttctat gataccaaat atagtattag atagagtgac agctgtaata
    15901 cctgtagtaa atatatcttt acttataaaa agtgtattat cttttaaaag cgatttaaat
    15961 ttaatagcct tagttttagc atcaaatgta gcctttgtta tattggcaat catactactg
    16021 tctaagatgt tcaattctga ggaaatactt tttggtaata gtaagagttt ctctttctta
    16081 gagaaaagaa gtaatataaa aaaagggtct ataccaactg taagtgatgg tgttatttta
    16141 tatgcagtag gattagttct attgatttat gttggttctt tagttcaaat taaatttggt
    16201 atggcaggta ttataatgac acaattcatg ataatttctt taccgttatt atttgcatac
    16261 tatattaaag ctgattttaa agaggtattt agcttgaaag ttcccaaatt aaagcatgtg
    16321 tttgggagca ttgttctttg gattggtgga tacatactta ttatgattat aacacagcta
    16381 attttattct tatttcctca aaatatggaa gttgctgaaa gtttaaataa ggctcttttt
    16441 atcaaagata gtgttttact gaatttacta gcaatagctt tattaccagc tttatgtgaa
    16501 gaaatgtttt ttaggggatt tatattttct tcatttagta aatcaaaaga taaaaataag
    16561 tctatcaagc tagctataat atgcagtggg gtactatttg gtattatgca tatggatttt
    16621 attagaataa ttccaacatc aatattaggt attatatttg catatagtgt gtataaatct
    16681 ggttcaatat ttgtatcaat gttattgcat tttttaaata atagtgtagc agttgtgtta
    16741 aatcattata caactggaaa tataaccaac ttatataaat ttgttgaagt tgatttttcg
    16801 aacttaaatg tacttaaatt tgccttaatt cttctaataa gtcttatatt aattatgtta
    16861 ggattaagag ttttagatag aaagtcttta agaaattaag tataaagtta ttatatagat
    16921 taaaatatta ataagcacta aagtatatgg atatatcttg tatataaaaa tactttagtg
    16981 ctatttt
//
LOCUS       DAELLV010000062        16883 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.062, whole genome shotgun sequence.
ACCESSION   DAELLV010000062 DAELLV010000000
VERSION     DAELLV010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16883
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.062"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(209..1828)
                     /locus_tag="KPD58_001663"
     CDS             complement(209..1828)
                     /locus_tag="KPD58_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBG0265435.1"
                     /translation="MNIVISLIGGLGLFLYGMSLMGEGLQKSAGDKLKKIIELLTSNV
                     VMGVLVGTVVTGIIQSSSATTVMVVGFVNAGIMNLSQAIGVIMGANIGTTVTAQLVSF
                     NLEGIAPIALGIGILFYLFTSNQKTKHLSEILIGFGILFTGMEFMKDAVAPLAEYKAF
                     TDALLYFSKNPVLGILAGFAITGIVQSSSASMGMLIALASQGILPLSSALPILYGDNI
                     GTCVTSLLSSVGASRNARRAAVMHLSFNVIGTIIFMLVLNKPISAIVTHFDPTDTARQ
                     IANAHTLFNLTNVIILLPFSKYIVKLANRLIPIKETESEIVNNTKYLDERMFSTPSIA
                     LGNTVQEVVRMGHKATNSLEHSIAGFLNKSNEDINKAFESEKVVNKLQKDILNYLLKL
                     SKEPLRDDERFRTDLLFNTVNDIERVSDHAENIAELAMSVKEMNISFSDSAIREIYEI
                     YNKTITNFKDALIVLDVKDFELANKVLEVENEVNYLEKTFRNSHMIRLNNGSCTIDAG
                     VLYLDLLTNLERISDHSTNIVKQVLKLKQKI"
     gene            complement(2137..2376)
                     /locus_tag="KPD58_001664"
     CDS             complement(2137..2376)
                     /locus_tag="KPD58_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0265436.1"
                     /translation="MNYNLIKVIFSLFKKKSGYTFTNFLNKVRVEKSKKYILKKDLSI
                     LDVAVLIGFNSQNYYSIIFKKFNNFTPIEYKNIYN"
     gene            2647..2838
                     /locus_tag="KPD58_001665"
     CDS             2647..2838
                     /locus_tag="KPD58_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265437.1"
                     /translation="MERLMELIERKFLKDVELLELEDIENVIECKMTQPYNAHYNEYL
                     VKVKNGDEVEEYFVYLKHR"
     gene            complement(2927..3739)
                     /locus_tag="KPD58_001666"
     CDS             complement(2927..3739)
                     /locus_tag="KPD58_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HBG0265438.1"
                     /translation="MADCNSCPSKGNCNSQSNCSIENNPNNKFGKIIGVMSGKGGVGK
                     STVTALLANKLNKMGYKVGILDSDITGPSIPRLMGVKNVKAYSDGSYIYPVENSNNIK
                     VMSINLMIDDENEPVVWRGPLLGGVVKQFYTDVLWEELDYLLIDMPPGTGDVALTVMQ
                     SIPISGIVMVSVPQDLVSMIVSKAVNMAKKMNINVLGVIENMSYIQCPDCSKKIKLFE
                     GESTEKFLDDLDLELLGELPMTKEIIDITHNGVTEISDDLDSILTNVVEKIK"
     gene            4161..5312
                     /locus_tag="KPD58_001667"
     CDS             4161..5312
                     /locus_tag="KPD58_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004452985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0265439.1"
                     /translation="MELRPRSAKLEEFDKVIELINYVFRISRNHKPTMMEEFPLLLSK
                     NNIENMIIISEDDKVVSDVNYLIQDVSIQGNRLKVAAIGGVCTHPDYEKRGYSSKILD
                     KVEEKMFYDGVDIVIISGTRSLYSRRNCSLVKSFYKYTIKPEDVKIAYEIVEFDETNF
                     EKDNDLDKMIELYNQNSTRFIRTRDEFQKLLHAATIAWGPIGYKKVFIKENNNIIGYL
                     IIRTIKKEDSTVGEVAEIGLNSVNVENILKYVANKFGLESLNYKVHVKNLKDQLKCNG
                     TKSLDYQQGTMKIINFTKLCDSLRSYFSQYVDFELLKYMEFKQVENKYIIKYKEEELV
                     IENLDKLNKLFFEKNEEQYNEFKHLKNIYEFATKAFPVDFPWTANLNYQ"
     gene            5430..6068
                     /locus_tag="KPD58_001668"
     CDS             5430..6068
                     /locus_tag="KPD58_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423639.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HBG0265440.1"
                     /translation="MDILDDIAECILNMGVDNIENLVKIAIDKNIDVEDIYEYGLNKG
                     MIGALDKFENKEYYLSEVIVCTDALNKGINLLKGTGKVKKKSKGVILMSVVEGDTHEI
                     GKNIVKVMVEATGYKVIDLGVNRKSEDIIEEAIKNNVDIIGLSSMMTTTMENMKSVID
                     ELNMIEIDKRPKVIIGGGPVSMEFAEEIGADGYSSNAPKAVKLINKLIGGEV"
     gene            6069..7091
                     /locus_tag="KPD58_001669"
     CDS             6069..7091
                     /locus_tag="KPD58_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HBG0265441.1"
                     /translation="MFSDMTLLQYINSEDRGFFLPDMGTNGLFLINKKAYEVYDSVDL
                     QLELAKTMDKYFESDFIYSFCDGITFCETLGLDTLRPDYDFPSVLTHTITDRDKLRKL
                     DVPDPYTSGRMPLNIESLSVISKSIKKPLYESIQGPFTLAGQLAGATQLLRCIITDKK
                     FVEELLEFTTELVRRYAVAANKAGAKYISIAEPTSVTLSKDRFDEFIVKNLNKIYNEL
                     DCWKGMHICGDTRELLDNMLSCNIDAVSLDQILDYEEIAPMIPRDIVLIGNLDPIKLL
                     GRSKPDKIRRETLKLLKKMRRYDNFLCDFGCNCLNTTPVENLQAAIKAGRISYKELDR
                     IDIDEL"
     gene            7081..7701
                     /locus_tag="KPD58_001670"
     CDS             7081..7701
                     /locus_tag="KPD58_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265442.1"
                     /translation="MNYNSNKNKYLLKDINLDDIFIDKNIVLKFLGYGNRKAPDTISK
                     IVEREIRNINDILDIKIYISEVDINKTPKECKKAFAVLYTIGDKIDFKMNDYMENCNM
                     MAGLALDKVGIVCLDCINEKIKMYLKGKYDSLKISHEIYPGDKDFEVERQKDIYNYVK
                     NKYNSIEIEINDYHQLSPIKSVAMLILMGDEENLESRCSKCPRKCF"
     gene            complement(7825..8511)
                     /locus_tag="KPD58_001671"
     CDS             complement(7825..8511)
                     /locus_tag="KPD58_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HBG0265443.1"
                     /translation="MKTYKFIFFDADDTLFDFKKSESHAFKKLLSEFDLEFNFENYIE
                     SYRNISDKLWLDLEKNIITLNELKLLRFELFANKISLDVDSETLSKMYLNFLGECTFL
                     IPGAIDILQYLKKKYTIVIITNGISAVQKKRLENSKIKEYIDGMVVSEELKISKPNPE
                     IFKYALKKFNCHDKSSALIIGDSLTSDILGGINSGIDTCWLNSNNSINYTNHIPTYEI
                     NTLIELKKLL"
     gene            complement(8525..9070)
                     /locus_tag="KPD58_001672"
     CDS             complement(8525..9070)
                     /locus_tag="KPD58_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0265444.1"
                     /translation="MNDLNLGEKIAEVRKKQNLSIRDLAKLADVTPSLLSQIERGLAN
                     PSVNSLKSIASSLNVPLFTFFVSEVDKKNLIVRHDNRKKVILPGSKEVIYEILIPDSS
                     GNLEFAIMDLAPNTSSCVDRITHNGDEIAYVLEGEVKLFMDDDEFTLSKGDSVKVPLG
                     TKHKWQNDSNSESKVIFAVIL"
     gene            9298..10545
                     /locus_tag="KPD58_001673"
     CDS             9298..10545
                     /locus_tag="KPD58_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBG0265445.1"
                     /translation="MQITTFGAIFGLLIAIILIIKKFQAVYSLMLGAFIGGLVGGANI
                     TQTVDFMANGAMNISPSILRALASGVLAGSLIKTGAVDKISEQIVKIFGEKRALFSIA
                     ISTMVLAGVGVNLDVSIITVAPIGLYIGRKLNYSKLSILLAMLGGGKAGNIISPNPNT
                     IAVADNFSVNLSSVMMANIIPAIIGVVITVILASILINKGNKVQSYEILEQREDLPSL
                     FKSLCGPIIAIFLLFLGNVSPIVIDPMIALPIGGIVTLIVTGNLNNSREYLAFGLSKM
                     QGVCILLLGTGTIAGIIQMSELQQSTIGALQFLNMPQFLLAPVSGILMSLATASSTAG
                     ATIASSTFHDAIINGGLSPISGASIVNAGSSVFEQLPHGSLFHTSAGSINMDIGERFK
                     LIPYEALIGIVMTIISTSIQLVL"
     gene            10632..12626
                     /locus_tag="KPD58_001674"
     CDS             10632..12626
                     /locus_tag="KPD58_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBG0265446.1"
                     /translation="MNKKKIVIIGIIYSFLVVFSLTNMYVNMEYNLNVFEYIKKSLPF
                     TEEEKKWLEKHKNLIYSSDQSSPPLRYKGKEDGQYKGIIVDLINSLSIQIGRDFYFKP
                     NNWWKESFVNSIDDSIKFFDLIPSKERANKFIFTDPIYTLSANILKDKKSQDINSYMD
                     LKGKTVAIPEGDYSINFLKQKIQDINILLTPDIKTGVNHLMSGKVDAVVGDEPVLRYY
                     INNYGLSNKYSVLSNPIYTKKAVLAVPKQYEELVSILNKGIFKLQKNGVYKDLKKKWY
                     STYNEVDDILYERGIVPSIYLFIGIILISIYVFYSYTYLLKIEIKRKTEQVIENKKTL
                     EATFNSITDIIMLVDENNNIVESNKVLYDFMGEMSYKIADLISMIKGVIENTFSENTN
                     KTSEIEIHNKILKINTFPVEYKKNNTEYIVVLIKDITNDKIVEAKLLRENKMISIGQL
                     ASGVAHEIRNPLGIIRNNCYLLKDNVTMEEVNDCVKSIESNVDRASNIITNLLNFARI
                     SDDNLEHINIRNFIENIVKLQYKMLQLKNVEIKIDCEHNLVCYINGESLKHVFINLIS
                     NSIDAIHQDGKIIIYCYEKNYCLFIDFKDNGEGIKEDALKDIFNPFYTTKPIGEGTGL
                     GLYITYNEIKKNNGDISVESKLGVGTCFHIKIPLNKEVTI"
     gene            12626..13948
                     /locus_tag="KPD58_001675"
     CDS             12626..13948
                     /locus_tag="KPD58_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBG0265447.1"
                     /translation="MKILLVDDELEYGVVMKKILQKKGYLVDIALSGEEAINIIKKDK
                     NYDLVLSDVMMKNMDGVQLLDRIKSINRDIEVILVTGYGSIENAVDAMKRGALSYFIK
                     SNPIENLLEEVEKVKASKISVSVQKNNLEFTLESKNRDFNNIIKIAKKAACKDVNILI
                     LGESGVGKDILARYIHSISPRKNEIFVPVNCCSFSENLLESELFGHEKGSFTGAVDSR
                     KGRFELSNKGTLFLDEIGDIPLNVQVKLLRTLEDKSIERIGSNKSIKVDFRLICAMNK
                     EPKVEISNGNIREDFFYRISTITITIPPLRKRREDLTTLIEFFLNKYQIEHDKKIHSI
                     DKEVKDFLLNYNYPGNIRELKNIINRLVVLSEEGNLSKDNLNLISNNVYIDDKISIRP
                     LREIRKEFECEYIEKVLSLCGNNISNTAKKLEISRRQLTNKISEYNIK"
     gene            14227..15513
                     /locus_tag="KPD58_001676"
     CDS             14227..15513
                     /locus_tag="KPD58_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HBG0265448.1"
                     /translation="MKLELGNIFIKNVEFGEKTEVKDGVLYISSEEIEKIVLEDERIV
                     SVNVELARPGESIRIAPVKDVIEPRVKIGDESKIFPGIINKVKTVGSGRTHVLLGACV
                     VTCGNIVGFQEGVIDMSGPTAKYTPFSKTNNICIVIKAKDGIDTHDYEEAARIAGLKI
                     GAYVGEAGREVEPDEVIVYETKPLLKQVKEYPDLPTVAYVHMLQSQGLLHDTYYYGVD
                     AKQIVPTFMYPTEIMDGAIISGNCVAPCDKVTTFHHLNNPVIHDLYKRHGKDLNFIGV
                     ILTNENVFLVDKERSSDMVAKLIEFLGVDGVLVTEEGYGNPDTDLMMNCRKCSEVGAN
                     VVLITDEFPGKDGKSQSIADATKEADAVVSCGQGNLVVHFPAMEKIIGTLDYVEMMIG
                     GYKGCLNEDGSMDAELQIIIASTIANGYNHLTARYY"
     gene            15599..>16883
                     /gene="grdB"
                     /locus_tag="KPD58_001677"
     CDS             15599..>16883
                     /gene="grdB"
                     /locus_tag="KPD58_001677"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:16646..16648,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HBG0265449.1"
                     /translation="MSKIRVVHYINNFFAGIGGEEKADIPPEKRAGVVGPGIAFQSQF
                     NDDAEIISTVICGDTYFGENIESATQELLKMIKEESPDLFIAGPAFNAGRYGVACGTI
                     CKAVEEELNIPVITGMYKENPGVDMFKLDLHIVSTGNSAASLRKVVPIMTKLGLKLVR
                     GEEIGPPEEEGYIMRGIRKNFFHELRGSERAIDMLVKKMKGESFETEYPMPEFDRVTP
                     MKPVKDLSKIKLALVTSGGIVPIDNPDKIESSSATKYGIYDLTDMDSMSNKEFTTIHG
                     GYDRAYVLENPNLVVPLDVVRELEKEGVIGELVDYFITTTGTGTSVGNSKRFGEEFSK
                     KLLEDDVDAVILTSTUGTCTRCGATMVKEIERAGIAVVHICTVVPISLTIGANRIVPA
                     VGIPYPLGNPMLGEEESKKIRKNIVLKALNALTEDI"
BASE COUNT         6614 a         1873 c         2753 g         5643 t
ORIGIN      
        1 tgtatacttg tgactttatt tatgggcagt tcaagtttta tgaaagaagt catatatata
       61 tggaatattc taggcagaaa ctgtttaata ataagagaat actataaaaa tttgagtatt
      121 acataaaaaa taagaagcca taaatgatat aagcattatc tatagcttct tacttctcat
      181 tgattaagat tatatataaa aataatcact atattttttg tttaagtttt agtacttgtt
      241 ttactatatt tgttgagtgg tcagaaattc tttctagatt agtaagtaag tcaagataaa
      301 gtacaccagc atcaattgta caacttccat tatttagtct aatcatatgt gaatttctaa
      361 aagttttttc taaataattt acttcatttt caacttctaa aactttattt gctaactcaa
      421 aatctttaac atctaaaact attaaggcat ccttaaaatt agtaattgtc ttattgtata
      481 tttcgtatat ttctcttatc gcactatcag aaaaactaat gttcatttcc ttaacactca
      541 tggccaattc agctatattt tctgcatggt cagagactct ttcaatgtca tttacagtat
      601 taaaaagcaa atctgttcta aagcgttcat catctctaag aggttcttta gaaagtttta
      661 acaaatagtt caatatatct ttctgtaact tattgacaac tttttctgac tcaaaagctt
      721 tattaatatc ttcattgctc ttatttaaaa atcctgcaat tgaatgttcc aaagagtttg
      781 tagctttatg acccattctt acaacttctt gtacagtatt accaagtgct atagatggag
      841 tgctaaacat cctttcatct aagtacttag tattatttac tatttctgat tcagtttctt
      901 taataggtat aagtctattt gctaatttaa ctatgtattt agaaaatggt aataatatta
      961 taacatttgt aagattaaat aaagtatgtg catttgctat ttgtctagct gtatcagttg
     1021 ggtcaaaatg agtaactatg gctgatatag gtttatttaa aacaagcata aatattattg
     1081 taccaattac attaaaactt aaatgcatta cagctgcacg tcgagcattt ctacttgcac
     1141 caacacttga taaaagtgaa gtaacacaag tacctatatt atctccataa agaataggta
     1201 aagcagatga tagaggaagt attccttgag aagctagtgc tattaacata cccatagatg
     1261 cacttgaact ttgaacaata ccagttatgg caaatcctgc caatatacct aatacaggat
     1321 ttttactaaa atataaaagt gcatcagtga atgctttata ttcagcaagt ggagctactg
     1381 catctttcat aaactccatt cctgtaaata atataccgaa acctataagt atctcagata
     1441 aatgctttgt tttttgattt gatgtaaata aatagaataa aatacctata cctaaagcaa
     1501 ttggtgcaat tccttcaaga ttaaatgaaa ctaattgagc agtaactgta gtacctatat
     1561 ttgcccccat tattacacca atagcttgac ttagattcat tatacctgca tttacaaatc
     1621 ctacaaccat aacagtagta gcacttgaac tttgtataat acctgtaacg actgtaccta
     1681 ccagtacacc cataacaaca ttacttgtta agagttcaat aatttttttt aatttgtctc
     1741 ctgctgattt ttgtaaacct tctcccatta aactcattcc gtaaagaaaa agtccaagtc
     1801 caccgataag gctaattact atattcacga tatttcctcc cttaagaaat taataatttt
     1861 tttatctgaa ttataaactt tattaactaa aatttattgt ttccataata tagaataaca
     1921 aattttgtca ataaaaatat taatgttttg ttaagtatac gttaagagaa tgttaaattt
     1981 ttaagttgtt gttaatttta gaaaatttat gatatatagc aatttaaata cttgaactta
     2041 gatttttagt attttatgta caaacaattt aacaaagaat taactataat tgtaaaaaat
     2101 atgtaaaaaa ataacatcct tttagaatga tattttttag ttataaatat ttttatattc
     2161 aattggtgta aagttattaa actttttaaa aattatacta tagtaatttt gactattgaa
     2221 tccaattagt acagcaacgt ctaaaataga caagtctttt tttaatatat actttttgct
     2281 tttttcaact ctaactttat ttaaaaaatt agtaaaagta tatccacttt ttttcttaaa
     2341 caaagaaaaa ataactttta ttaagttgta attcattaca tatattactt actgttatat
     2401 cttctgagta atttttgtga atataattta tagttttctt taagttttct ttacataaaa
     2461 ctaagagttg gtctatgttt cattttatca aggcatattt attaaaaaat caatctctat
     2521 ttgttaaaat aatttatgaa tgatgttaga aactgtaata tattggatat aatataataa
     2581 acagataaac tctgcattaa gaggatatat agtttatctt aattataggg gaagtgagga
     2641 tatattatgg aaagattaat ggaactcata gaaagaaaat ttttaaagga tgtagaactt
     2701 ttagaattag aagacataga aaatgtaata gaatgcaaaa tgacacagcc atataatgct
     2761 cattataatg aatatttagt aaaagtaaaa aatggagatg aagtagaaga atattttgtg
     2821 tatttaaaac atagataaac ataaaagagg tttatattta tttgtatgat tataaaataa
     2881 atacaagcct ctttttttga taaatataaa attataaaat ttttatttat ttaatttttt
     2941 caacaacatt agtcaatatt gaatctaaat catcactaat ttctgtaact ccattatgag
     3001 taatatctat aatttcttta gtcataggaa gttctcctaa aagctctaag tctaaatcat
     3061 ctaaaaactt ttcagtagat tctccttcaa ataacttgat ttttttagaa caatcaggac
     3121 attggatata actcatattt tcaataactc caaggacatt tatgttcatt tttttagcca
     3181 tattaacagc ttttgataca atcatagata ccaaatcttg aggtactgaa accataacta
     3241 ttccacttat aggaatagat tgcataactg ttaaggcaac atctccagta ccaggaggca
     3301 tatctattaa taaatagtct aattcttccc aaagtacatc agtataaaat tgctttacaa
     3361 cccctccaag taaaggtcct ctccaaacaa caggttcatt ttcatcatct atcataaggt
     3421 ttatagacat tacttttatg ttatttgagt tttctacagg gtaaatataa gagccatcag
     3481 aataagcttt tacatttttt acacccataa gtctaggaat acttggtcca gttatatctg
     3541 agtctaatat accaacctta tatcccattt tgtttagctt attagcaagt aaagcagtta
     3601 cagttgattt tccaactcca cccttcccac tcataacacc aattattttt ccaaatttat
     3661 tgttagggtt attttcaata gaacaatttg attgactatt acaatttccc tttgatggac
     3721 atgaattaca atctgccata tctattacct ccaaacttta atatttatta gattatttgt
     3781 aacctacata tactatttta gtacaattga accataaagt cattatttaa aagcaactta
     3841 gaagattttc tattgattta ttcatagact atagcaaaaa gaatacaata aaaaaattat
     3901 aacattcttg aaaaagtgat tatttcaatt tagttataga acttagtcat ataaatatat
     3961 agaaaatatt ttaatatatt atacataacg ggttatttta taagtttaac tataaattag
     4021 gtttatattt tataaaaatt tgctaaaaaa aacaattaaa aattgtaaag aaaaaagata
     4081 taattagaac tgtagtaata aatgttttat tatagataat agagttttaa gtatcttaaa
     4141 taatatttgg aggaatatga atggaattaa gaccaagaag tgcaaaatta gaagaatttg
     4201 ataaagtaat agaattaata aactatgtat ttagaatatc aagaaatcat aaaccaacta
     4261 tgatggaaga atttcctctt ttactaagta aaaataatat agaaaatatg attataatat
     4321 cagaagatga taaagtggtt tctgatgtaa actatctaat tcaggatgta tctattcaag
     4381 gaaacagatt gaaggttgca gctataggtg gagtatgtac tcatccagat tatgaaaaga
     4441 gaggctattc atctaagata ttggataaag tagaagaaaa aatgttttat gatggtgtag
     4501 atattgtaat tatatcagga acaagaagtc tatacagtag aagaaattgt agtttggtaa
     4561 agagttttta taaatataca ataaaacctg aagatgtaaa aatagcatat gaaatagttg
     4621 aatttgatga aactaatttt gaaaaagata atgatttaga taaaatgatt gaactatata
     4681 atcaaaatag tactagattt ataagaacga gagatgagtt tcaaaaacta ttgcatgcag
     4741 caactattgc ctggggacca attggttaca agaaggtatt tataaaggaa aataataata
     4801 taataggata tttgataata agaacaataa aaaaagaaga ttctactgta ggagaagttg
     4861 ctgaaatagg gttaaactct gtaaatgttg aaaatatatt gaaatatgtt gcaaataaat
     4921 ttggattaga atctttaaat tataaagtac atgtgaaaaa tcttaaagac cagttgaaat
     4981 gtaatgggac aaaatctttg gattatcaac aaggaactat gaaaataata aattttacta
     5041 agttgtgtga tagtttaaga agttatttta gtcaatatgt agattttgaa ttactaaaat
     5101 acatggaatt taaacaagtt gaaaataaat atattattaa atacaaagaa gaagaacttg
     5161 taatagaaaa tttggataag cttaataaac tgttttttga aaaaaatgag gaacagtaca
     5221 atgaatttaa acatctaaaa aatatatatg aatttgctac aaaggcattt cctgtagatt
     5281 ttccatggac tgcaaattta aattatcaat agttttagat gatattaaat aaaattgatt
     5341 gatacctaga gttagtcctt gttaaataag agaaattatt ggatattgaa aaagcaattt
     5401 tgtaagtaat tagaaaggat gagtattata tggacatact tgatgatata gcagaatgta
     5461 tattaaatat gggtgtagat aatatagaga atttagtaaa aattgctata gataaaaata
     5521 ttgatgtgga agatatttat gaatatggtt taaacaaagg tatgatagga gctttagata
     5581 agtttgagaa taaggaatac tatctttcag aagttatagt atgcacagat gcattaaata
     5641 aaggtattaa tcttttgaag ggaactggaa aagtaaagaa aaaatcaaag ggagtaattc
     5701 tgatgtctgt agtagaagga gatactcatg agataggaaa aaatattgta aaagtaatgg
     5761 tagaagccac tggatataag gttattgact taggtgtaaa tagaaaaagt gaagatataa
     5821 tagaagaagc tataaaaaac aatgtagata taataggctt atcttcaatg atgacaacta
     5881 caatggaaaa tatgaaatcg gttatagacg aacttaatat gattgagata gataaaagac
     5941 caaaagtaat aattggtgga ggacctgtaa gtatggagtt tgcagaagaa ataggagcag
     6001 atggatacag ttcaaatgct ccaaaagcag ttaaattaat aaataaactt ataggaggtg
     6061 aagtttaaat gttttcagat atgactttac ttcagtatat aaatagtgaa gatagaggtt
     6121 tttttctgcc tgatatgggt acaaatggat tgttcttaat aaataaaaag gcatatgaag
     6181 tatatgatag tgtagatttg caattagaat tagcaaagac tatggacaag tattttgaat
     6241 cagattttat atactcattc tgtgatggga ttacattttg tgaaactctg ggtttagata
     6301 cattaagacc agattatgat tttccaagtg tattgacaca tactataact gacagagata
     6361 aattaagaaa attagatgtt ccagaccctt atacgagtgg acgtatgcca ctcaatatag
     6421 agagcttatc agttatatca aaaagtatta aaaaaccact atatgaatcg atacaaggtc
     6481 catttacact tgcaggtcaa ttagctggag ctactcaact tttaagatgt ataataactg
     6541 ataaaaaatt tgtagaagaa ttattggaat ttacaactga attggtaaga agatatgctg
     6601 tagctgcaaa taaagcagga gcaaaatata tttcaatagc agagccaaca tcagtaaccc
     6661 taagtaaaga tagatttgat gaatttatag taaaaaattt aaataaaatc tacaatgaac
     6721 ttgattgttg gaaaggtatg catatatgtg gagacaccag agaattatta gataatatgc
     6781 taagttgtaa tatagatgca gtaagtttag accaaatttt agactatgaa gaaattgctc
     6841 caatgattcc tagagatata gttttgattg gtaatttaga cccaataaaa cttcttggac
     6901 gttctaagcc agataagatt agaagagaga cactaaaatt gttaaagaag atgagaagat
     6961 atgataactt tttatgtgat tttggctgta actgcttaaa taccactcca gtagaaaatt
     7021 tacaagcagc tataaaggca ggaagaatta gttataaaga attagatagg attgatattg
     7081 atgaattata atagtaataa aaataaatat cttttaaagg atataaattt agatgatatt
     7141 tttatagata agaatattgt gctaaagttt ttggggtatg gaaatagaaa agctccagat
     7201 acaattagta aaatcgttga aagagaaata agaaatataa atgatatttt agatatcaag
     7261 atatatataa gtgaagttga tattaataaa acacctaaag aatgtaaaaa agcatttgct
     7321 gtactatata caatagggga taaaattgat tttaagatga atgactatat ggaaaattgc
     7381 aatatgatgg cagggctggc attggataag gtaggtatag tatgtttaga ttgtattaat
     7441 gagaaaataa aaatgtattt aaaaggaaag tatgatagtc tcaaaatatc gcatgaaata
     7501 tatccaggag ataaagattt tgaagtagag agacaaaagg atatttataa ctatgtaaaa
     7561 aataaatata atagtataga aatagaaatt aatgattatc atcaactatc ccccataaaa
     7621 agtgttgcaa tgcttatttt aatgggggat gaagaaaatt tagaatcaag atgtagtaag
     7681 tgtcctagaa aatgctttta gttaattttc aagactaaga tttgttttgt gtataaattg
     7741 caataatttt tttggtcatt ttatgtataa actaaaaaaa ttattgcaaa gtaatattct
     7801 atttaatttg aacagactta caaactatag tagtttcttt aattcaataa gtgtatttat
     7861 ttcataagtt ggtatatgat tagtataatt tatactgtta ttggaattta accaacaagt
     7921 atctattcct gaatttattc cacctaagat atcagaggtt aagctatccc caattattaa
     7981 ggcagaagat ttatcatgac aattaaactt tttgagagcg tatttaaata tttcaggatt
     8041 tggttttgat attttcaact cttcagatac aaccattcca tcaatatatt cttttatttt
     8101 tgaattttca agtcttttct tttgaactgc agaaattcca tttgttatta taacaatagt
     8161 atactttttt ttaagatatt gtaatatatc gattgcacct ggaattaaaa atgtacattc
     8221 acctaaaaaa tttaagtaca ttttgctaag agtttcagaa tcaacatcca aagatatttt
     8281 atttgcaaaa agttcaaatc taagtaattt aagttcattc aaagttataa tatttttttc
     8341 aaggtcgagc caaagcttat cacttatatt tctatatgac tctatataat tctcaaaatt
     8401 aaattctaaa tcaaactcag atagaagttt tttaaaagca tgtgattcac ttttcttaaa
     8461 atcaaataaa gtatcatctg catcaaaaaa tataaattta tatgtcttca tggtttttac
     8521 tcctttacaa gatgactgca aaaattactt tactttcact atttgagtca ttttgccatt
     8581 tatgttttgt tcctaaagga acttttacac tatctccttt ggacaaagta aattcatcat
     8641 catccataaa aagtttaact tctccctcca aaacatatgc aatttcatct ccattatgag
     8701 taatcctatc aacacaagaa gaagtgtttg gagctaaatc cataattgca aattctaaat
     8761 taccactaga gtctggaatt aaaatctcat atataacttc tttgcttcca ggtaaaataa
     8821 ctttttttct attatcatgt cttactatca agtttttttt atctacctca gatacaaaaa
     8881 atgtaaataa aggaacgttt agagaagagg ctatagattt taatgaattt acagatggat
     8941 tagcaagacc tcgttctatt tggctcaata atgatggtgt aacatcagct agttttgcta
     9001 aatctcgaat acttaagttt tgtttttttc gaacttcagc tattttttcc cctaaattta
     9061 aatcattcat ataaatatcc tctcttttta atttaaaaaa tattaactta aaataatcaa
     9121 gtaaaatgat tatgtagtat gaaattatta taacatatag aattaggttt tgaaaaaact
     9181 atgttcaata taattaaata aattaaaata taattgacaa agtggataca gaatacttaa
     9241 aatatattta aataaaatta agcaaaagat agttaattta tgataggagg ggatattatg
     9301 caaataacga cttttggagc tatatttggt ttattaatag caattatttt aattataaaa
     9361 aaatttcaag ctgtatatag tttaatgcta ggagcattta ttggaggatt agttggagga
     9421 gcaaatataa cacaaactgt agattttatg gcaaatggag ctatgaatat atcaccatca
     9481 attcttagag ctttggcatc aggtgtatta gctggaagtt taataaaaac aggggcagta
     9541 gataaaatat cagaacagat tgtaaaaata tttggtgaaa agagggcttt attttcgatt
     9601 gccatttcaa ctatggtact tgctggagta ggcgtaaatt tggatgtatc tataataact
     9661 gtagcaccta taggattata tataggtaga aaattaaatt attctaaatt gtcaatacta
     9721 ttagctatgt tgggaggggg taaagcaggt aatataatat caccaaatcc aaatactata
     9781 gcagtagcag ataatttttc tgtcaatctc tctagtgtta tgatggcaaa tataattcca
     9841 gcaattattg gagttgttat tacagttatt ttagcaagta tattaataaa taaaggaaat
     9901 aaagtacaat catatgaaat tttagagcaa agagaagatt taccaagttt atttaaatca
     9961 ttatgtggtc caattatagc tatatttcta ttgtttttag gaaatgtatc tcctatagtt
    10021 atagacccaa tgatagccct tccaatagga ggaatcgtaa ctctaattgt cacaggaaac
    10081 ttgaacaatt caagagaata tttagctttt ggattatcta agatgcaagg ggtatgcata
    10141 ttattacttg gaacagggac tatcgcagga attatacaga tgtctgaatt acaacagagt
    10201 acaataggtg ctttacaatt tttaaatatg ccacaatttt tattggcacc agtttcgggt
    10261 atacttatgt cattagcaac agcatcatca acagctggtg ctactatagc atcatctact
    10321 tttcatgatg ctataataaa tggtgggcta tcaccgattt caggtgcctc aatagtaaat
    10381 gctgggtcta gtgtattcga gcaattacca catggctcac tatttcatac tagtgcaggt
    10441 agtataaaca tggatatagg tgaaagattt aagttaattc catatgaagc attgatagga
    10501 atagtaatga ctataatttc aacatctatt caattagttt tataataaat ataaatattt
    10561 aatgaaaaaa gtaggatttt gttattaaat attgaattta ttatagtaaa aaagaagatt
    10621 ggagttttaa agtgaataaa aaaaagattg ttattatagg gattatttat tcatttttag
    10681 tagtattttc acttacaaat atgtatgtaa atatggagta taatctaaat gtatttgaat
    10741 atattaagaa gtcacttcca tttacagaag aagaaaaaaa gtggttagaa aaacacaaga
    10801 atttgattta cagctctgac caaagttcac ctcctttgag atataaaggt aaggaagatg
    10861 gacagtataa gggaattatt gttgatttaa taaattctct gtcaattcaa attggtagag
    10921 atttttattt taaaccaaat aattggtgga aagaatcttt cgtaaattca atagatgata
    10981 gtataaagtt ttttgacttg attccatcaa aagaaagggc aaataaattt atatttactg
    11041 acccaatata cactttgagt gcaaatatat tgaaagataa aaagtcccaa gatataaata
    11101 gttacatgga tttaaagggg aaaacagtag caattccaga aggtgattat tctataaatt
    11161 ttttaaagca aaaaattcaa gatataaaca ttttgttgac tccagatata aagactggag
    11221 tcaaccattt aatgtctgga aaagtagacg cagttgttgg tgatgaacca gtattgcgat
    11281 actatataaa taattatgga ctttcaaata aatacagtgt tttaagtaat cctatatata
    11341 ctaaaaaagc agttcttgct gtacctaaac aatatgaaga gctggttagt attttaaata
    11401 aaggcatttt taaacttcag aaaaatggag tgtataaaga tttaaagaaa aaatggtatt
    11461 ctacgtataa tgaagttgat gatatattgt atgagcgagg aattgtgcct agtatttatc
    11521 tttttatagg tataatttta atatcaattt atgtttttta tagctataca tatttgttaa
    11581 agatagaaat caaaaggaaa acagaacagg taatagaaaa taaaaaaact ttagaggcaa
    11641 cttttaatag tattacagat ataataatgc ttgtagatga aaataacaat atagtagaat
    11701 cgaacaaagt actatatgac tttatgggtg aaatgagtta taagatagca gatttgatta
    11761 gcatgataaa aggtgttatt gaaaatacct tctctgaaaa tactaataaa actagtgaaa
    11821 tagaaataca caataaaatt ttaaagataa acacttttcc tgtagagtat aaaaaaaata
    11881 atactgagta tatagtagtt cttattaaag atataactaa tgataaaata gtagaggcga
    11941 aattacttag agaaaacaaa atgatatcta taggacaatt agcttctggg gtagcacatg
    12001 agataagaaa tcctttgggt ataataagaa ataattgcta tctcttaaaa gataacgtta
    12061 caatggaaga agtaaatgat tgtgttaaat caatagaaag taatgtagat agggcaagta
    12121 atataataac caacttgtta aatttcgcaa gaatatcaga tgataattta gaacatataa
    12181 atataagaaa ttttattgaa aatatagtga aacttcaata taaaatgtta cagttaaaga
    12241 atgttgaaat aaaaattgac tgtgaacata atttagtatg ctatataaat ggagaatcgt
    12301 taaaacatgt atttataaat ttaatttcta attcaataga tgcaatacat caagatggaa
    12361 aaattattat atactgttat gaaaaaaatt attgtttatt tatagatttt aaagataatg
    12421 gtgaaggaat aaaagaagat gccctaaagg acatatttaa tcccttctat acaacaaaac
    12481 caattggtga gggaactggt ttaggtttat acattactta taatgagatt aaaaagaata
    12541 atggagatat aagtgttgaa agtaaattgg gggttggaac ttgttttcat ataaaaattc
    12601 cattaaataa agaggtgact atataatgaa aatactatta gttgatgatg agctagaata
    12661 tggagtggta atgaaaaaaa ttctacaaaa gaagggctat cttgtagata tagcactaag
    12721 tggtgaagaa gctataaata taattaaaaa agataaaaat tatgatttgg ttttgtctga
    12781 tgttatgatg aaaaatatgg atggagtaca gctattagat agaattaaat ctattaatag
    12841 agatatagag gttatattag ttacaggata tggaagtatt gaaaatgcag ttgatgctat
    12901 gaaaagagga gctttatctt attttataaa aagtaatccc attgaaaatt tattagaaga
    12961 agttgaaaag gtaaaggcaa gtaaaatatc tgtttctgta caaaaaaata atctggaatt
    13021 tactttggaa tctaaaaata gagattttaa taatatcata aaaatagcaa aaaaagctgc
    13081 ttgtaaagat gttaatatat taatcttggg ggaatctggt gtaggaaaag atatattagc
    13141 tagatatata catagcataa gtccaagaaa gaatgagata tttgtacctg taaattgttg
    13201 ttctttttca gaaaatctat tggagtctga gttgtttggt catgaaaaag gttcttttac
    13261 aggagcagta gatagtagaa aaggtagatt tgaactttcg aataaaggaa ctctattttt
    13321 ggatgaaata ggagatatac ctctaaatgt tcaggtaaaa cttttgagga cacttgagga
    13381 caaatctata gagaggatag gaagtaataa atctattaaa gttgacttta ggttaatttg
    13441 tgctatgaat aaagaaccaa aagtggaaat atcaaatgga aatataagag aagatttttt
    13501 ttatagaata agtacaataa caataactat accaccgcta agaaagagaa gagaagactt
    13561 aactactctt atagaatttt tcttaaataa gtaccaaatt gaacatgata aaaagataca
    13621 ctcgattgat aaggaagtaa aggatttttt attaaattac aattatccag gaaatataag
    13681 agaattaaaa aatataataa atagacttgt agttttaagt gaagaaggca atttatcaaa
    13741 agacaattta aatttaatat caaataatgt atacatagat gataaaataa gtattagacc
    13801 tttaagagaa ataagaaaag agtttgaatg tgagtatatt gaaaaagttt tatctttgtg
    13861 tggaaataat atttcaaata cagctaaaaa attggagatt agtagaagac agttaaccaa
    13921 taaaatatca gagtataata ttaagtgaga aaaaaacttc tcattaagtg agaaaaaaat
    13981 ttctcaaact gtattaaaag ataaacagaa aaaatctgtt tatctttttt tattataatt
    14041 ttaaaaatta atatttacaa taattattgc taaattcaat attggtgtaa attaaaaatg
    14101 aaaatttcta tgacattaat acatagaata tatattttaa ataattggca tagaaaatgc
    14161 tttatatatt aataaacgta ataaatactt ttaattgcga ttaaatatat taagggggat
    14221 tttcatatga agctagagct agggaatata tttataaaaa atgttgaatt tggagaaaag
    14281 acagaagtaa aagatggagt actatatatt agtagcgaag aaatagaaaa gatagtatta
    14341 gaagatgaaa ggattgtaag tgtaaatgtt gaacttgcaa gacctggcga atcaataaga
    14401 atagctccag ttaaagatgt aatagagcct agagttaaaa ttggagatga aagcaaaata
    14461 tttcctggaa ttataaataa agtgaagact gtaggaagtg gaagaaccca tgtactttta
    14521 ggagcttgtg ttgtaacttg tggaaatata gttggatttc aagaaggcgt aattgatatg
    14581 agtggtccta ctgctaaata tacacctttt tcaaaaacaa ataatatatg tattgttata
    14641 aaagcaaagg atggtataga tactcatgat tatgaagaag cagctagaat tgcaggatta
    14701 aaaataggtg catatgtagg tgaggcagga agagaagttg agccagatga agtcattgta
    14761 tatgagacaa agccattatt aaaacaagta aaagaatatc cagacctgcc aacagtagca
    14821 tatgttcaca tgttacaatc acaaggactt ttacatgaca cttattacta tggggtggat
    14881 gctaaacaga tagtgcctac atttatgtat ccaactgaaa taatggatgg agctataata
    14941 tctggaaact gtgtagcacc atgtgataag gttacaacat tccatcattt aaataatcca
    15001 gttatacatg acttatataa aagacatgga aaagatttaa actttatagg agttattttg
    15061 actaatgaga acgtattctt agtcgataaa gagagaagct ctgatatggt agctaaatta
    15121 atagaatttt taggagttga tggagttcta gtgacagaag aaggttatgg gaatccagat
    15181 actgacctaa tgatgaattg tagaaaatgt agtgaagttg gagcaaatgt agttttgata
    15241 acggatgaat ttcctggaaa agatggcaag agtcaatcta tagcagacgc tactaaggaa
    15301 gcagatgcag ttgtttcttg tggtcaagga aatctagtgg ttcatttccc agcaatggaa
    15361 aaaattatag gaactttaga ttatgttgaa atgatgatag gtgggtataa aggatgctta
    15421 aatgaagacg gaagcatgga tgcagagcta caaattataa tagcatcaac tatagcaaat
    15481 ggctataatc atttgacagc aagatattat taaataagta tttgaatata aagttctgtt
    15541 gatataatca ttggtctaat tgatgtaaca actataaagt gatttggagg attaagagat
    15601 gagtaaaatc agagtggttc attatataaa caatttcttt gctggtatag gtggagagga
    15661 aaaagcagat attccgccag aaaaaagggc tggtgtagta ggacctggaa tagcatttca
    15721 gagtcaattt aatgatgatg cagagataat ctcaactgta atttgcggag atacgtattt
    15781 tggtgaaaat attgagagtg caactcaaga acttttaaaa atgataaaag aagaaagccc
    15841 tgatttattt attgcaggcc cagcatttaa tgcaggtaga tatggagtag cttgtgggac
    15901 tatttgtaag gcagttgaag aagagttaaa tattcctgta attacaggca tgtataaaga
    15961 aaatccaggt gtagatatgt ttaaattaga tttgcatata gtgtctacag gaaattcagc
    16021 agcaagtctt agaaaggtag ttccaataat gacaaaacta ggtcttaaat tggttagagg
    16081 tgaggaaata ggacctcctg aagaggaagg atatataatg agaggaataa gaaaaaattt
    16141 cttccatgaa cttagagggt ctgaaagagc aattgatatg ttggttaaaa agatgaaagg
    16201 ggaaagcttt gaaactgagt atccaatgcc tgaatttgat agagttacac ctatgaaacc
    16261 tgttaaagat ttatctaaga taaaacttgc cctagtaact tctggaggga tagttccaat
    16321 tgataatcca gacaaaattg aatcttcaag tgctactaaa tatggaattt acgatttaac
    16381 agatatggat tctatgtcaa ataaagaatt tacaactata catggggggt atgacagagc
    16441 atatgtcctt gaaaatccaa atttagttgt accactagat gtagttagag agcttgaaaa
    16501 agaaggtgta attggagaat tagtagatta ttttataact actacaggaa caggaacgag
    16561 tgttggcaat tctaagagat ttggagaaga gttttctaaa aaattgttag aagatgatgt
    16621 agatgctgta attttaactt ctacatgagg tacttgtact cgttgtggag caacgatggt
    16681 aaaagagatt gagagagctg gtatagcagt tgttcatata tgtacagtag taccaatatc
    16741 acttacaata ggagctaata gaattgttcc agcagtaggg ataccatatc cacttggaaa
    16801 tcctatgtta ggagaagagg aaagtaaaaa aattagaaag aatatagttt taaaagcttt
    16861 aaatgcacta acagaagata tag
//
LOCUS       DAELLV010000063        16860 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.063, whole genome shotgun sequence.
ACCESSION   DAELLV010000063 DAELLV010000000
VERSION     DAELLV010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16860
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.063"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            90..251
                     /locus_tag="KPD58_001678"
     CDS             90..251
                     /locus_tag="KPD58_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265450.1"
                     /translation="MIIPSRRIQDITLTNLKNGEVTLIELDEIYKKMGFLFVVSEGKL
                     KKIKKENHH"
     gene            444..1073
                     /locus_tag="KPD58_001679"
     CDS             444..1073
                     /locus_tag="KPD58_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBG0265451.1"
                     /translation="MKIVTYNIHKGMDSNNRLTLTKMGLYLKQLDCDVICLQEVLYPQ
                     FLALKAVLNMDGVFATNVKKVNMIYGICTFTTSKMLNSNHFFLTSKKEQRGALCITID
                     AYGRIINVINTHLGLDRQERAKQLDEIIDYRNRLVGIVILCGDFNEKNVFLSMFNDMA
                     ISLNKSYLPTFEKSNSRIDYIFVNKNTELKGYTVEKIYLSDHYPVIGYI"
     gene            1312..2571
                     /locus_tag="KPD58_001680"
     CDS             1312..2571
                     /locus_tag="KPD58_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBG0265452.1"
                     /translation="MGRRTTRVSRLGKEKKRSVLIVLAALVLIMGGFTYFFNSKFLYN
                     GKIAKNVYIEGVNVSDMTKAEALKAITDKYTPEDLNLAYDGKKYTISPKDIDLKYDTE
                     DVVKDAYESTKKGSYFQNLKKYIDIRVNKANMKIKAEYDEAKLSSKVSSIADSINVKM
                     KNASISVGSGGLSYTDSVVGREFDLAANKESIYNMIKNKEHKTLELKVNLQKPDITTE
                     QVKTVNSVIGQYSTTYSQAVEGRSYNVGLSARKTSDVLLMPGEEFSYNKLTGPSNKAN
                     GYKDAPVIVYGKLEQSAGGGVCQTSSTVYNAALLSGMEITQVTNHSSASTYVPKGRDA
                     TVSDGGLNLKFKNPYKHPVYIKNYAGGGSVSSVIYGNSGDKPNISIEVKQTGQNKYST
                     YRIFKDSSGKVIKKEHISNSSYKELKK"
     gene            2830..3825
                     /locus_tag="KPD58_001681"
     CDS             2830..3825
                     /locus_tag="KPD58_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HBG0265453.1"
                     /translation="MYEEVTWLNFIGNRYEVVNSDDVLEINKIYKARDVFYRKNVLIK
                     VIKHNNYICEDFVSNLIDESTALDEINSPYILKIIDVGIHCTEETTLYYVVSEDFNGI
                     GLDELILGNYLHLEAIVNIMIQVLKALEMIHRNHSYHGSLKSSSIIVDTEYNIKICDF
                     GITKANNGVNTRSYGNRRYLCPHQLCINYTDKESDFFATGLILFESIFKKLPFGESNS
                     EEKMLQSIDKGLDWNSIKAINGNTELINVIKRLLGRNNKYTRANDIIIDLSKVMYEKA
                     YIEVEDEKEEEQKQDKVIQVKKEYKSKMRKKLHKKLVVAGLAIVMISMIIISSII"
     gene            4071..5138
                     /locus_tag="KPD58_001682"
     CDS             4071..5138
                     /locus_tag="KPD58_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HBG0265454.1"
                     /translation="MSNIKIIASNKCWIEDIARKQLEDISKLNGILRVVGLPDLHAGK
                     IPVGLAVETKNIIYPHIIGNDIGCGMTLFKTGVLKKKFKKDKWIKSLSKIKDLSDIEI
                     KNTYKEECPILNLGTIGSGNHFVEIQCISEIYNKEQFEQLKFSSDDIMMLVHCGSRNY
                     GEDILKKFYDKDGLEVESEKAIDYIKKHDNALMWAERNREAISKKIMQSIGTSGDVET
                     IFSINHNFVEKREDKFIHRKGAVSSERGAVIIPGSRGSLSYIVMPTENTEISLYSLSH
                     GAGRKWSRSVCKSRLKSKYSKDTIKQTKFKSQIICNDVNLLFQEAPEAYKNIEQIIES
                     LIEYELIQVVATMKPLITYKG"
     gene            5672..7582
                     /locus_tag="KPD58_001683"
     CDS             5672..7582
                     /locus_tag="KPD58_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBG0265455.1"
                     /translation="MNSNTFERLQLNEVKELIKIHCVSSLGKNLIDKLTPSGNIGVVR
                     RKLAENKEARKIIENSNHIPLEGLFNAGSTIDKIEKGMIIEPVELVNIEDFLRGCRKM
                     KAFMLEKEFYSPTLSSYALNITECKSIEDEINYCIKSNKVDSNASKELKKIRRNIEIT
                     EGKIKDRLNKFITSTVNKKYIQEFIISKRNDRYVIPIKSSYKNEVNGTILDTSSKGNT
                     VFIEPISVSNLSTELTMLKADETIEEYKILSYLTELIFEKISQIKLNIEILSEYDMLF
                     AKAKYSQKIKGITPKINNNGYIKIIKGKHPLLTGDAVPLDFEIGKNYRSLIITGPNAG
                     GKTVTLKTVGLLTLMVQCGFDISAKEGSEFSVFEKVFVDIGDNQSIENALSTFSSHIK
                     NIAEIMSLSNNSTLVLFDEIGSGTEPNEGAGLAISLLEEFYKMGCITIASTHYGEIKK
                     FATLHPEFENAGMMFDKETLEPLYKLTIGKSEDSNALFISKKMGIKNRVLDRAKEYVI
                     DRNYNLDLVKRSKLQSNIEDKNSSEVILNYTDYNVGDKVKLLDEDDFGIVYKPMDKFN
                     NVEVLFNDNFVSVNIKRLQLSIKASELYPEGYDLDVLFTSFKERKLEKDIQRGSKKAL
                     KKIQKEIRENRK"
     gene            complement(7645..8268)
                     /gene="ytaF"
                     /locus_tag="KPD58_001684"
     CDS             complement(7645..8268)
                     /gene="ytaF"
                     /locus_tag="KPD58_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation membrane protein YtaF"
                     /protein_id="HBG0265456.1"
                     /translation="MIESILLVTSLCIDAFVTSFGYGVNKVKVPLYSNIIISVVCSAI
                     LAISLFAGTIVRDFLPQNITMLICFFILFLLGSARLIESFFKSYFKKKSIHSKNYNFN
                     MLDLKLNFNVSIEPDVLDTSKSKVLKSAEAFTLALALSLDGLAIGFGSGLVGVNYIQV
                     ILFSLISNIIAVLLGCLLGNMFVKNVNLNLSWLSGLILLVIGFMKIC"
     gene            8456..9100
                     /locus_tag="KPD58_001685"
     CDS             8456..9100
                     /locus_tag="KPD58_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0265457.1"
                     /translation="MEQNNKVGNFGDLLKILLDEKELSMGELSKKSGIDKSTISRIAN
                     NKQKPNINHLEKMAIHLNINLEELLKASGYEFRNSNNQIFNADSDFSNFDDILGFANL
                     INDKNFNGNIEKELSKCKLYVQTDEGKKLLFDNFNKKIDSIEKQGQFTDRLRQMYADF
                     CTDGLTIKKYLLIGSMLLYFVISTDVIPDFVFPIGFMDDLVALNIVTKLLKNDK"
     gene            9314..10372
                     /locus_tag="KPD58_001686"
     CDS             9314..10372
                     /locus_tag="KPD58_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HBG0265458.1"
                     /translation="MNCKWISKIDERKKCHREADSSGYCIFHKENKSDEEIQLMMDTL
                     HKEEISEFNGFVFENEFNAEEILTYNYKILDFSESIFKQKANFKKYIFKKNIIFNYTE
                     FRDKVLFNGCVFLENCDFNRTIFSKHYINDRIFEKVKFKGPDLVVNKVENFPRMDGII
                     FSMCTKFVLKNVEYGKSEYEHGKINYRIARNQATKIGEYEMIGFYYYKERIYSSKIMK
                     RSNYPTFSDYLVEKFFDQIARYTTGYGEKPWNILLVIIAIISVFALLYLFVGIESSNS
                     TLVALDINNIGDYSLSEIFKMYMDLWYFSMATFSTVGYGDMVATSLIGKALAGIEVFF
                     GVTIGAIWASVIIKRMIR"
     gene            10476..11660
                     /locus_tag="KPD58_001687"
     CDS             10476..11660
                     /locus_tag="KPD58_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBG0265459.1"
                     /translation="MKNALKKIISLAMILVFIIPNYSYADDKSVDKYSKTSILIDQET
                     GRVLYSKEPDMKVPLASLSKMMTFLLAIEAIENKEVKETDKVKIDKSIASVKGSSYKL
                     KDGEEVPLIELMRGLMIVSGNDAAIAIAKHVCKTKEAFVDRMNKKAKEIGMSNTHFLN
                     PNGLPIYDLSNPKKPAKENISTAKDIAILGKYMFDHYEKQVTAITDMETYTNSDRSFE
                     KSNTNALLRIIPEVDGIKTGYTGNAGYCLSFSMVVNKNDKNEKKRRVIGVVLGTNHKN
                     KRTSASLALLKYGKENFNMKKVIDKDSFIGKKYIKGTKELEVTLKAKDELYTILKDDE
                     SIKSEVKIKNIEYPVKKGDTMGIIKYYTNSGDLLGSVDIVSNNSIDNVSLKTKLKMKF
                     AN"
     gene            11867..12580
                     /gene="rpsB"
                     /locus_tag="KPD58_001688"
     CDS             11867..12580
                     /gene="rpsB"
                     /locus_tag="KPD58_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBG0265460.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIYIIDLQ
                     KTVKKVEEAYKFTKEVAETGKPILFVGTKKQAQDAIKDEAERCGMYFVNERWLGGMLT
                     NHKTIKTRINKLRELEKMEEEGVFNVLPKKEVIKLRAEKEKLEKYLGGIKDMPELPGA
                     MFVVDPRKENIAIQEAHRLGIPVVGIVDTNCDPEQLDFAIPGNDDAIRAVKLITGAMA
                     TAVIEGRQGAEEEVAEDQE"
     gene            12659..13570
                     /locus_tag="KPD58_001689"
     CDS             12659..13570
                     /locus_tag="KPD58_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBG0265461.1"
                     /translation="MANITAQMVKELRESTGAGMMDCKKALQEAEGNMEKAVDLLREK
                     GLSKAAKKAGRVAAEGLVAIEMNDDNTVASMVEVNSETDFVAKNEDFKVFVKDAACMA
                     LATDKEDIASLLGETHKEGITLQEVLNNRVAKIGEKLDFRRFAKVVTNGQVAGYIHGG
                     GKIGVLVEMETEARDAKVLELGKDVAMQVAAMNPKYVSRDEVDAEYIAHETEVLTQQA
                     LNEGKPANIVEKMVKGRLEKELKEVCLLEQTFVKNPDITVKQLVADVAKAVGSDIKVV
                     KVVRFEVGEGIQKREENFAEEVAKQLK"
     gene            13655..14359
                     /locus_tag="KPD58_001690"
     CDS             13655..14359
                     /locus_tag="KPD58_001690"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBG0265462.1"
                     /translation="MDKPMYKRVLLKLSGEALAGERGFGINNDVVNDIAIAIKKIQEI
                     GVEVAVVVGGGNFWRGRTSEGMDRTTADYIGMLATVMNAMALQDALENIDVATRVQTA
                     IDMRQIAEPYIRRRAVRHLEKERVVIFGAGTGNPYFTTDTTAALRAAEMEAEVILLAK
                     NVDAVYDKDPKVHADAKKFTELSYMEVIQKELKVMDSTATSLCMDNKIPIKVFELTTE
                     NIIRAVKGENIGTTVK"
     gene            14385..14942
                     /gene="frr"
                     /locus_tag="KPD58_001691"
     CDS             14385..14942
                     /gene="frr"
                     /locus_tag="KPD58_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBG0265463.1"
                     /translation="MKLEIHKQLEEKMNGTIDALKFEFGTIRAGRANAQMLDKIRVDY
                     YGTPTPINQIGAISVPEPRILMISPWDKSAMHEIEKAIANSDLGLNPSNDGEVIRLSV
                     PALTEERRKELAKKASKAAEEFKVRIRNERRDANEKIKKMEKGGELTEDELKKAQDEV
                     QKMTDKFIKEIDTLLSKKEKDIMEV"
     gene            14945..15148
                     /locus_tag="KPD58_001692"
     CDS             14945..15148
                     /locus_tag="KPD58_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265464.1"
                     /translation="MKKYYNLLGLHVDEVKQYFDEQNINYTVKSIEGKKDKEKLVVPR
                     VIKISEIGDSVELIITYFSDSLV"
     gene            15177..15911
                     /locus_tag="KPD58_001693"
     CDS             15177..15911
                     /locus_tag="KPD58_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HBG0265465.1"
                     /translation="MNNNIIYDIDLNNIPTHIAIIMDGNGRWAKARFLPRTAGHKAGV
                     ETIRDIVKECSKLGVKHLTLYAFSTENWKRPKLEVDTLMNLLSTYLRNEIAELHQNNV
                     KVTAIGDISALPKTCIRELNSAKELTKNNTGVNLNLALNYGSRADIKNALIDIVKNCE
                     SGKIDINNIDEDIIKNYLSTKSIPDPDLVIRTSGEQRLSNFLLWEVAYSEFYFTDIHW
                     PDFKKEELQKAIYVYQRRDRRFGGLK"
     gene            15924..16703
                     /locus_tag="KPD58_001694"
     CDS             15924..16703
                     /locus_tag="KPD58_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBG0265466.1"
                     /translation="MLTRIIASLALVPLFLFVVYGGIPLYIAETAIVYIALHEFYKAF
                     KIKDVHPIFIIGYLFSIYLAVKNIFNLPLEYTYAVIFILFLASIIYMLMGKNNVIDVS
                     ITFLGVFYIGVFLDFIIITINGFEKGSIYVWLIFVISFMTDIFAYFSGYLLGKHKLIP
                     KVSPKKTIEGAIGGIIGSTLCCILFGYLFGIDLLQLAIIGSIGSVIAQLGDLFASSIK
                     RYVGIKDYGKIIPGHGGILDRFDSVILVAPFVYSAIKFFIR"
BASE COUNT         7059 a         1677 c         2920 g         5204 t
ORIGIN      
        1 tattttaata atttatgaat atatatcaga atgcataaat atatttaaat ataaagatta
       61 aaaagaattt aaaggagtgt tgataactta tgataattcc atcaagaaga atacaagata
      121 taacattaac aaatttaaaa aatggtgaag taactttaat tgaattggat gaaatataca
      181 agaagatggg atttttattt gttgttagtg aaggcaaatt gaaaaagata aaaaaggaga
      241 atcatcatta gaaatgaatg aaataataaa tattataata gtttataaaa cctaacttat
      301 gtagaatata aatacttaag ttaggttttt ttgtgataaa gtttataagt actcaatttt
      361 ataagataaa taatggtctt aagtgaaaag taaataagtt taaattttag taagtattat
      421 attgcataag aggtgatatt attatgaaaa tagtaaccta caatattcat aaaggtatgg
      481 attcaaataa tagattaact ctaactaaaa tggggttata tttaaaacaa ttggattgtg
      541 atgtaatttg tttgcaagaa gtattatatc ctcaattttt agctttaaaa gctgttttaa
      601 atatggatgg tgtctttgcg acaaatgtaa aaaaagtaaa tatgatatat ggtatttgta
      661 catttacaac atctaaaatg ttaaacagta atcatttttt cttaactagc aaaaaagaac
      721 aacggggagc tctttgtata actatagatg catatggaag aattataaat gtaataaata
      781 ctcacttggg acttgatagg caggagagag caaaacaatt agacgaaata atagactata
      841 ggaacagatt agtggggata gttattttat gtggtgattt taatgagaaa aatgtctttt
      901 taagtatgtt taatgatatg gctatttcac taaacaagtc ttacttacca acatttgaaa
      961 aatcaaactc tagaatagat tacatttttg ttaataaaaa tactgaacta aaaggataca
     1021 cagtagaaaa aatttacctg tcagaccatt atcctgtaat aggttacatt taaatttcaa
     1081 tataaatgaa attagttgtt ataaaataaa caattttttg ttaagaaata gtaaaagaaa
     1141 tataataaaa aatatcaaaa ataaaactaa ttataagaca taaatttaca ataaagttac
     1201 atttgattaa attccttgtt taacaaaagc tattcaaata taatctaagg tagttttgaa
     1261 atgggaactc tattatgcaa atttaagcta gtgttaaaat gaggtgaagt tatgggcaga
     1321 agaacaacaa gagtttcaag attaggtaaa gaaaaaaaac gaagtgtttt aattgtattg
     1381 gcagctttag ttctaataat gggtggattt acttattttt ttaacagtaa atttctgtac
     1441 aatgggaaaa tcgctaaaaa tgtatatata gaaggagtaa atgtatctga tatgactaaa
     1501 gcagaggcat taaaagctat aacagataag tatactccag aagatttaaa tcttgcttat
     1561 gatgggaaaa aatatacaat atcaccaaaa gatattgatt taaaatatga tacagaagat
     1621 gttgtaaaag atgcttatga aagtacaaaa aaagggtcct attttcaaaa cttaaaaaaa
     1681 tatattgata taagagtgaa taaggcaaat atgaagataa aagctgaata tgatgaggca
     1741 aaacttagtt ccaaagtgtc ttctatagca gatagtataa atgttaagat gaaaaatgct
     1801 tctattagtg ttggaagtgg tggactaagt tatacagact ctgttgtagg tagagaattt
     1861 gatttggcag caaataaaga gtctatatat aatatgataa aaaataaaga gcataaaact
     1921 ttagaactta aagtaaatct tcaaaaacca gatataacaa ctgaacaagt aaaaactgta
     1981 aattctgtta taggtcaata ttcaactact tattcacaag ctgtagaggg taggtcatat
     2041 aatgtaggat tatcagcaag aaaaactagt gatgtattat taatgccagg tgaagaattt
     2101 tcatacaata aacttacagg acctagtaat aaagcaaatg gttacaaaga tgcacctgtt
     2161 atagtttatg gtaagctaga acaatcagca ggaggaggtg tttgtcaaac atcttcaaca
     2221 gtttataatg cggctcttct ttcgggtatg gagataactc aagttacaaa tcattcatct
     2281 gcatctactt atgtgcctaa agggagagat gctactgtga gcgatggagg attaaactta
     2341 aagtttaaaa atccatataa gcatcctgta tatattaaaa attatgcagg tggaggaagt
     2401 gtatcatcag taatctatgg taattctggt gacaaaccaa atatatctat agaggtaaaa
     2461 caaactggtc agaataaata ttctacatat cgaatattta aagattcaag tggaaaggtc
     2521 ataaaaaaag aacatatatc aaatagttct tataaagaat taaaaaaata gataattttt
     2581 tagagaaata tttgtaatat aaaagggagt gtctcaaaat gaacttttta gtttatgagg
     2641 cactcttttt ctgtgaaatc aaatatattt tcatcatttc aacaatacaa aagaagcgta
     2701 tctctatttt gagacagcct tttttgtaaa aattaacact attagttctt gttttaatat
     2761 tacaatcatt aatatataga aaatattcct atatattggt ataattgtaa atagatttag
     2821 ttatataaaa tgtatgagga ggtgacatgg ttgaatttta taggcaatag atatgaggta
     2881 gtaaattctg acgacgtttt agaaattaac aaaatctata aagctagaga tgttttttac
     2941 aggaaaaatg tgttaataaa agttataaag cacaataatt atatctgtga agattttgtt
     3001 tcaaatttaa ttgatgaaag tactgcttta gatgagataa actctcctta tatactaaaa
     3061 ataatagatg ttgggattca ctgtacagaa gaaacaacat tatattatgt tgtaagtgaa
     3121 gactttaatg gtatcggact agatgaatta attctaggta attatcttca tttagaagca
     3181 attgtaaaca taatgataca agttttaaaa gctttagaaa tgatacatag gaatcattca
     3241 tatcatggta gtttaaaatc gagtagtata atagtagata cagaatacaa tataaaaatt
     3301 tgtgattttg gaattacaaa agcaaataat ggggttaata caagaagcta tggaaacagg
     3361 agatatttat gcccacatca actatgtata aattatactg ataaagaaag tgattttttt
     3421 gcaacaggtc ttattctatt tgaatctata tttaaaaaat tgccttttgg agaatcaaat
     3481 tcagaagaaa aaatgctaca atctattgat aagggattag attggaatag tataaaagca
     3541 attaatggaa atactgaact tataaatgtt ataaaaaggc ttcttggaag aaataataag
     3601 tataccagag ccaatgatat tattattgat ttaagtaaag taatgtatga aaaagcatat
     3661 atagaagttg aagatgagaa agaagaagag cagaaacaag acaaagttat acaagttaaa
     3721 aaagaatata aaagtaaaat gagaaaaaaa ttacataaaa aacttgttgt tgccggtcta
     3781 gctatagtca tgatttcaat gattattatt agttccataa tataaaaatt gtatattaag
     3841 tattttaaaa tcaatgtcta taaaggatat acatagaaat tttagtgtag tcttaaatta
     3901 tgaaaggaag tggttattat gttaacaaaa aataatgagg tattaaacgt ctaggagtat
     3961 tttaacaaat agccacctgc ttttacagtt tagaataaaa aagctgtggc taaaggtcac
     4021 agctttttgc tactttttaa aggcaggaga atattaaaaa ggagcaaatt atgagcaata
     4081 taaaaataat agcaagtaat aaatgttgga ttgaagatat agcaaggaaa caacttgaag
     4141 atatttcaaa gttaaatgga attttaagag tagtaggatt acctgactta catgcaggta
     4201 aaatacctgt aggtttagca gttgagacta aaaatattat ttatccacat ataattggaa
     4261 atgatatagg atgcggaatg actttattta aaactggagt tctaaaaaag aaatttaaaa
     4321 aagacaaatg gattaagtca cttagtaaga ttaaagattt gtctgatata gaaataaaaa
     4381 acacatataa agaagaatgt ccaattttaa atttaggaac tatagggagt ggaaatcatt
     4441 ttgtagaaat tcaatgtata agtgaaattt ataataaaga acaatttgaa caattaaaat
     4501 tttcatctga tgatattatg atgttagttc actgtgggtc aagaaattat ggagaagata
     4561 ttttaaaaaa attttatgat aaagatggac ttgaagttga aagtgagaaa gctatagatt
     4621 atattaaaaa acatgataat gctttaatgt gggcagagcg taatcgtgaa gctatcagta
     4681 aaaaaattat gcagtcaata ggtacatctg gagatgttga aacaatattc tctataaatc
     4741 ataactttgt agagaaaagg gaagataaat ttatacatag aaaaggtgct gtttcaagtg
     4801 aaagaggagc tgtgattata ccaggttcaa gaggcagtct atcatatata gttatgccta
     4861 ctgaaaatac agagatatct ttatattctc tatcacatgg agcaggtagg aaatggtctc
     4921 gttcagtttg taaatctcgc cttaaaagta agtatagtaa agatactata aaacaaacta
     4981 aatttaaaag tcagattata tgtaatgatg taaatttatt atttcaagaa gctccagagg
     5041 catataagaa tatagagcag attattgaaa gtttgataga atatgaactt attcaagtag
     5101 tggcaactat gaagccattg attacttata aaggataatt tttaaagtta aaaaagatta
     5161 aaattatatt ataaatagct aattttatgg aacatttaaa agtgttttat tattagctat
     5221 ttttttattt tgtagagacg ataatttata gagctaaatt ctttgatata taatgattac
     5281 aaagttagac tgtattggaa actgcacata catgaattga tgacaatata agtgattcca
     5341 aaatataaat gaaagtataa gcttattttc actataaaaa ttttcactgt acaaaaacat
     5401 taatgacatt atttttattg acataaatat ataatttatg gaataatatt agaagcactg
     5461 atataagtgt ttaaataagg atagaaaagg agaagtgttt tatgaaaaaa aatactaaca
     5521 ttaatgagtc cggagttaaa cttcttaata aataaggagt ttgatatata ataactcctt
     5581 atttattaag ttgttcgcaa aataactatt atcaaagtaa atgatagggt gatttaaaag
     5641 acgataactt ataaataaag gagaaaaaat aatgaatagt aatacatttg aaagattaca
     5701 actaaatgaa gttaaagaat taataaaaat acattgtgta agttcactag gaaaaaattt
     5761 aatagataaa ttaactccta gtgggaatat tggagtagta agaaggaaat tagcagaaaa
     5821 taaagaagct agaaagataa ttgaaaatag taatcacata ccacttgaag gattgtttaa
     5881 tgcaggttct acaatagata aaattgaaaa aggaatgata atagaacctg ttgaacttgt
     5941 aaatattgaa gattttttaa gaggttgtag gaagatgaaa gcttttatgt tagaaaaaga
     6001 attttattca cctacattaa gttcttatgc cttaaatata acagaatgta aaagtataga
     6061 agatgagata aattactgta taaaatctaa taaagttgat tctaatgcta gtaaagaatt
     6121 aaaaaagata agacgaaata tagaaattac agaaggaaag ataaaagata gattaaataa
     6181 atttataaca tcaactgtaa ataaaaagta tatacaagag tttataataa gtaaaagaaa
     6241 tgatagatat gtaataccaa ttaaatcttc ttataaaaat gaagttaatg gtactatatt
     6301 agatacatca tcaaaaggaa atactgtatt tattgaacct attagtgttt caaatttaag
     6361 tacagagtta actatgctaa aagctgatga aactattgaa gaatacaaaa tattatctta
     6421 tttaacagaa cttatatttg agaagataag tcagataaaa ttaaatatag agatactttc
     6481 agaatacgat atgttatttg caaaagctaa gtatagtcaa aaaataaaag gaattactcc
     6541 aaaaataaat aataatggct atataaaaat aattaaaggt aaacatccac ttctaacagg
     6601 agatgcagta cctttagatt ttgaaatagg caaaaattat agaagtctta taataacagg
     6661 tcctaatgca ggaggaaaaa ctgtaaccct taagactgtt ggtttactaa ctttaatggt
     6721 tcaatgtgga tttgatattt ctgcaaaaga aggtagtgaa tttagtgtat ttgaaaaagt
     6781 ttttgtagat ataggggata atcaaagtat tgaaaatgcc ttaagtactt tttcatcaca
     6841 tataaaaaat attgcagaga ttatgagttt atcaaataat tctactcttg ttctgtttga
     6901 tgaaatagga agtggaactg aacctaatga aggagctggt ttagcaatat cattattaga
     6961 agagttttac aaaatgggat gtataactat agcatcaact cattatggag agataaaaaa
     7021 gtttgctaca ttgcatccag aatttgaaaa tgctggtatg atgtttgata aagaaacgtt
     7081 agaaccattg tataagttaa ctattggaaa atctgaagat agtaatgctc tttttatttc
     7141 taaaaaaatg ggaataaaaa atagagtttt agatagagct aaagaatatg taattgatag
     7201 aaactacaat ttagatttag ttaaaagaag taagctacaa tctaatattg aagataaaaa
     7261 tagttctgaa gtcattttaa actatacaga ttataatgta ggggataagg taaaattact
     7321 agatgaagat gattttggaa ttgtatataa gcctatggac aagtttaata atgtagaagt
     7381 attgtttaat gataattttg tttcagtaaa tataaaaaga ttacaactta gcataaaagc
     7441 atcagaattg tatccagaag ggtatgattt agatgtatta tttactagtt ttaaagaaag
     7501 aaaattagaa aaagatattc aaagaggttc aaaaaaagct cttaaaaaga ttcagaaaga
     7561 aattagagag aatagaaaat aaatataaaa agcacaaaag aaagcaatct aaagagaaaa
     7621 gtttatcttt agattgcttt cttattaaca tatcttcata aatccaatga ctaacaagat
     7681 taaaccactt aaccaagata agtttaaatt tacgttttta acaaacatat ttcctagtaa
     7741 acaaccaagt aatactgcaa ttatatttga tattaaagaa aatagaataa cttgtatata
     7801 attcacacct acaaggccac taccaaaacc gatagctaat ccatctaaac ttaaagcaag
     7861 tgcaagtgta aatgcttcag cagatttaag cactttagat ttacttgtat ctaagacatc
     7921 aggttcgata cttacattaa aatttaattt aaggtctaac atattaaaat tataattctt
     7981 tgagtgtata gattttttct taaaataaga tttaaaaaaa ctttcaatta gtctagcact
     8041 tcctaataaa aataaaataa agaaacatat taacattgtt atattttgag gtaaaaaatc
     8101 tctaactatt gtacctgcaa aaagcgatat tgctaaaata gctgaacaaa ctacgctgat
     8161 aattatattt gaatataagg gaactttaac tttatttaca ccatatccaa agcttgtcac
     8221 aaaggcatct atacaaagag aagtaactaa taaaattgat tcaatcacag tagattaaca
     8281 tcctttctct taaattttgt acttatatta gttatagtat tgtaaaaatg gataaattgt
     8341 tacagataat ccaataaatt gttgataaaa ttccaacatc atacaacacc taaataacaa
     8401 aagaatcata tcatataata atataaaacc aatcataata aagcgggtga aaataatgga
     8461 acaaaataat aaggttggga atttcggtga tttgttgaag attttattag atgaaaaaga
     8521 attatcaatg ggagaactaa gtaaaaaaag tggaattgat aaatctacta tatctcgtat
     8581 agctaataat aaacaaaagc caaatataaa tcatttagaa aagatggcaa tacatttaaa
     8641 tattaaccta gaagaactgt tgaaagcttc aggatatgaa tttagaaaca gcaataatca
     8701 aatatttaat gcagatagtg attttagtaa ctttgatgat attcttggat ttgcaaattt
     8761 aatcaatgat aagaacttta atggaaatat tgaaaaagag ttaagtaagt gtaagttata
     8821 tgtccaaaca gatgaaggta aaaaattact ttttgataat tttaataaaa aaatagattc
     8881 tattgaaaaa caaggtcaat ttacagatag attaagacaa atgtatgctg atttttgtac
     8941 agatggactt acaataaaga aatatttatt gattggaagt atgctacttt actttgttat
     9001 atcaacagat gtgattccag acttcgtgtt cccaatagga tttatggatg atttagtagc
     9061 attaaatata gttacaaaat tacttaaaaa tgataagtaa gttttagaat catatttaat
     9121 atataaataa atcaaagtat ataaaattta ttatttttta tatgaaacaa attaaaagca
     9181 caatgaagta gattaaaaat ttaaaactaa gaatctaata tatattttgt aactatttaa
     9241 gtatataaaa taaaataata tccaaactaa tacaaaatag taataattgt tttgtactgg
     9301 aggatatata tttatgaatt gtaaatggat atctaaaata gatgagagaa aaaaatgtca
     9361 tagagaagca gattcttctg gatattgtat ctttcataaa gaaaataagt ctgatgaaga
     9421 gatacagttg atgatggata ctcttcataa agaagagata agtgaattta atggatttgt
     9481 ttttgaaaat gaatttaatg cggaagaaat attaacttac aattataaaa tactggattt
     9541 ttctgaatcc atatttaaac aaaaagcaaa ttttaaaaaa tatattttta aaaagaatat
     9601 tatttttaac tatacagaat ttagagataa agttttattt aatggttgtg tattcttaga
     9661 aaactgtgat tttaatagaa ccatatttag taaacactat ataaatgacc gtatatttga
     9721 aaaagtgaaa tttaaaggac ctgatttggt agttaataaa gtagaaaatt ttccacgaat
     9781 ggatggaata atttttagca tgtgtacaaa atttgtgtta aagaatgttg aatatggaaa
     9841 aagtgagtat gagcatggca agataaatta tagaatagct agaaatcaag caacaaaaat
     9901 tggagaatat gagatgatag gattttacta ttataaggaa agaatttata gtagtaaaat
     9961 aatgaaacgt tctaattatc ctaccttcag tgattattta gttgaaaaat tttttgacca
    10021 aatagcacgt tacacaacag gttatggaga aaagccatgg aatatattat tagttattat
    10081 agcaataata agtgtttttg ctttattata cttatttgta ggcatagaaa gttcaaatag
    10141 cacacttgta gctttagata taaataatat aggtgattac tcactaagtg agattttcaa
    10201 aatgtatatg gatttatggt attttagcat ggctacattt agtacagtag gatatggaga
    10261 tatggttgct acaagtttga ttgggaaagc attggcaggt atagaagtat tttttggagt
    10321 tacaatagga gcaatttggg catctgtgat tattaaaaga atgataaggt aatatttgtg
    10381 taaatatgga taggtattaa ttactagata taaattggac taaataggga aaatttaaat
    10441 ataaatatac ttaataatcc aagaaggtga aaaatttgaa aaatgcacta aaaaaaatta
    10501 tatcattagc catgattcta gtttttataa taccaaatta ttcatatgca gatgataaat
    10561 ctgtagacaa gtattcaaaa acatctatac ttatagacca agaaacaggt agagttttat
    10621 atagtaaaga acctgatatg aaggtaccat tagctagttt aagtaagatg atgacttttt
    10681 tacttgcaat agaagctatt gaaaataaag aagtaaaaga aactgataag gtaaaaatag
    10741 acaaatctat tgcatcagta aaagggtcaa gttataaact taaagatggt gaagaagtgc
    10801 cacttataga gcttatgaga ggtcttatga ttgtatcagg aaatgatgcg gcaattgcta
    10861 tagcaaaaca tgtttgtaaa acgaaagaag catttgtaga tagaatgaat aaaaaagcaa
    10921 aagaaatagg aatgagtaat acacattttc taaatccaaa tggtcttcca atatacgatt
    10981 taagcaaccc caaaaaacca gcaaaagaaa atatctctac tgcaaaagat atagctattc
    11041 ttggaaaata catgtttgac cattatgaaa aacaagtaac agctataact gatatggaga
    11101 catatacaaa ttcagataga agttttgaaa aaagcaatac aaatgcatta ttaagaatta
    11161 taccagaagt agatggtata aaaactggat atactggcaa tgctggatat tgcttatcat
    11221 tttctatggt tgtaaataaa aatgataaaa atgagaaaaa acgtagagta ataggggttg
    11281 tattaggtac taaccataaa aataaaagaa cctctgcttc attagctcta ctgaaatatg
    11341 gaaaagaaaa tttcaatatg aaaaaagtaa ttgataaaga tagctttatt ggtaagaaat
    11401 atataaaagg aacgaaagag ttagaggtta cattaaaagc aaaagatgaa ctttatacaa
    11461 tattaaaaga tgatgaaagt ataaagtcag aagttaagat taaaaatata gagtaccctg
    11521 ttaaaaaagg tgatactatg ggtattataa aatattatac taattcaggt gacttacttg
    11581 gaagtgtaga tatagtaagt aataatagta tagataacgt atcattaaaa acaaaattaa
    11641 aaatgaaatt tgcaaattaa attcaaaaaa tggtataaaa tacacaaaaa aatataaaaa
    11701 tagaaaatat ggaaaaaata ttgttatttc ttcacctctg tgatatacta ctagaggtga
    11761 aaaaataaaa taaatcacac acagcattgg gatccttaaa aggtgcctat tacatggttt
    11821 tttagggaga agatcaagct ggaggtataa aaccaggagg taagatatgt cagtaatatc
    11881 aatgaaacaa ttattagaag caggtgttca ctttggtcac caaacaagaa gatggaaccc
    11941 taaaatggct aagtatatat tcacagagag aaatggaata tatataatcg acttacaaaa
    12001 aacagttaaa aaagttgaag aagcttacaa gttcactaaa gaagtagctg aaacaggaaa
    12061 gccaatctta ttcgtaggaa ctaaaaaaca agctcaagat gcgataaaag atgaagctga
    12121 aagatgtgga atgtactttg ttaatgaaag atggttaggt ggaatgttaa caaaccacaa
    12181 aactataaaa acaagaataa ataaattaag agaattagaa aaaatggaag aagaaggcgt
    12241 atttaatgtt cttcctaaaa aagaagttat aaaattaaga gctgaaaaag aaaaactaga
    12301 aaaatactta ggtggaataa aagacatgcc tgaattacca ggtgcaatgt tcgtagttga
    12361 cccaagaaaa gaaaacatag ctattcaaga agctcataga ttaggtatac cagtagttgg
    12421 tatagttgat actaactgtg acccagaaca attagacttt gctataccag gaaatgatga
    12481 cgctataaga gccgtaaaat taataactgg tgctatggca acagctgtaa ttgaaggtag
    12541 acaaggtgct gaggaagaag tagctgaaga ccaagaataa taatttgaca attaaatggt
    12601 aagggtcgcc cttaccattt ataatatgga atagaataag taggaggaaa cttaaaaaat
    12661 ggctaatata actgctcaaa tggtaaaaga gttaagagaa agtacaggcg caggaatgat
    12721 ggactgcaag aaagctttac aagaagctga aggaaacatg gaaaaagcag tagatttatt
    12781 aagagaaaaa ggattatcaa aagctgctaa aaaagctggt agagttgctg ctgaaggtct
    12841 agttgcaata gaaatgaatg atgataatac agttgcttct atggtagaag ttaactcaga
    12901 gacagatttc gttgctaaaa acgaagactt caaagtgttc gttaaagatg ctgcttgtat
    12961 ggcattagct actgataaag aagatatagc ttctttatta ggtgaaactc ataaagaagg
    13021 aataacttta caagaagttt taaataatag agttgcaaaa ataggtgaga aattagactt
    13081 tagaagattt gctaaagttg taactaatgg acaagttgct ggatacattc acggtggtgg
    13141 aaaaataggt gttcttgttg aaatggaaac agaagctaga gatgctaaag ttttagaatt
    13201 aggtaaagat gtagctatgc aagttgctgc tatgaatcct aagtatgttt caagagatga
    13261 agtagatgct gaatacatag cacatgaaac agaagtatta actcaacaag ctttaaatga
    13321 aggaaaacca gctaatatag ttgaaaaaat ggttaaagga agattagaaa aagaattaaa
    13381 agaggtttgt ttattagagc aaactttcgt taaaaatcca gatataactg ttaaacaatt
    13441 agttgctgat gttgctaagg cagtaggttc tgatataaaa gttgttaaag ttgtgagatt
    13501 cgaagttggt gaaggtatac aaaagagaga agaaaacttt gcagaagaag ttgctaagca
    13561 acttaagtaa taaccaaaga gagaacacgc aggtgttctc ttttttaaaa aatagaaaaa
    13621 attggatatg aaggattgaa ataggaggtt tctgatggat aaaccaatgt acaaaagggt
    13681 attgttaaaa ttaagtggag aagcattagc aggagaaaga ggttttggta taaataatga
    13741 tgtagttaat gatattgcta ttgctataaa aaaaatacaa gaaataggtg ttgaagtagc
    13801 tgtagtagta ggtggaggaa acttctggag aggcagaacc agtgaaggaa tggacagaac
    13861 tactgctgac tatataggaa tgttagctac agttatgaat gcaatggcgc tacaggatgc
    13921 actagaaaat atagatgttg ctacaagagt tcaaactgct atagacatga gacaaattgc
    13981 agaaccatat ataagaagaa gagcagttag acacttagaa aaggaaagag ttgttatatt
    14041 tggtgcagga acaggaaacc cttactttac aacagataca actgctgctt tacgtgctgc
    14101 tgagatggaa gcagaagtta tattgttagc taaaaatgtt gatgcagtat atgacaagga
    14161 tccaaaagta catgcagatg ctaagaaatt tacagaatta agttacatgg aagttataca
    14221 gaaagaatta aaagtaatgg attctacagc tacatcttta tgtatggata ataaaattcc
    14281 aataaaggta tttgagctta caacagagaa tataataaga gcagtaaaag gcgaaaatat
    14341 aggaactact gtaaaataat gaagaatgaa ggaggagtaa aattatgaaa ctggaaatac
    14401 ataagcaatt agaagagaaa atgaatggga caattgatgc tttaaagttt gaatttggaa
    14461 ctataagagc aggaagagct aatgcacaaa tgctagacaa aataagagtt gattattatg
    14521 gaactccaac tccaataaat caaataggtg ctatatctgt accagaacca agaatattaa
    14581 tgatatctcc ttgggataaa tctgctatgc atgaaataga aaaagcgata gctaactcag
    14641 acttaggatt aaatccatca aatgatggtg aggttataag actttctgta cctgctttaa
    14701 cagaagaaag aagaaaagaa ttagctaaaa aagctagtaa agctgcagaa gaatttaaag
    14761 ttagaataag aaatgaaaga agagacgcca atgagaaaat taaaaagatg gaaaaaggcg
    14821 gagaattaac tgaagatgaa ttaaaaaaag cacaagatga agttcaaaaa atgactgata
    14881 aatttataaa agaaatagat acattattat ctaaaaaaga aaaggacata atggaggtat
    14941 aatattgaaa aaatattata accttttagg tcttcatgta gatgaggtaa aacaatattt
    15001 tgatgagcaa aatataaatt ataccgtaaa atctattgaa ggtaaaaagg ataaagaaaa
    15061 acttgtagtt cctagagtta taaaaatatc tgagataggt gatagtgtag aactgataat
    15121 cacatacttt tctgactcct tagtttaagg agtcagaaca atattggagg caaaatatga
    15181 ataacaacat tatatatgat atagacttaa ataacatacc tactcatatt gctattataa
    15241 tggatggaaa tggaagatgg gcaaaagcta gatttcttcc aagaactgca ggacataagg
    15301 ctggagtaga aactataaga gatatagtaa aagaatgctc aaaattaggt gttaagcact
    15361 taacattata tgcattttca acagaaaact ggaaaagacc gaaacttgaa gttgacacac
    15421 ttatgaactt attatctaca tacttaagaa atgaaatagc tgaacttcac caaaacaatg
    15481 taaaagtaac tgctatagga gatataagtg ctttaccaaa aacttgtata agagaattaa
    15541 actcagctaa ggaactgaca aaaaacaata caggagttaa cttaaattta gcattaaact
    15601 acggaagtag agcagatata aaaaatgctc ttatagatat agtaaaaaat tgtgaaagtg
    15661 gtaaaataga tataaacaat attgatgaag atattataaa aaactattta agcacaaagt
    15721 ctataccaga tccagacttg gtaataagaa caagtggtga acaaagattg agtaactttt
    15781 tactttggga agttgcttat tctgagtttt actttactga tatacattgg cctgatttta
    15841 aaaaagaaga actacagaag gctatatacg tatatcaaag aagggataga agatttggag
    15901 gacttaagta aggagattat tttatgctta caagaattat tgcttcacta gctctggttc
    15961 cattatttct atttgttgta tatggaggta ttcctctata tatagctgag acagcaatag
    16021 tttatattgc attgcatgag ttttataagg catttaaaat aaaagatgta catccaatat
    16081 tcattatagg atatttattt tctatttatt tagctgtaaa aaatattttt aatttaccat
    16141 tagaatatac ttatgcagta atttttattt tatttttagc aagtataatt tatatgttga
    16201 tgggcaaaaa caatgttata gatgtttcga ttacgttttt aggagttttt tatataggag
    16261 tatttttaga ttttataatt ataactataa atggatttga aaaaggtagt atatatgtat
    16321 ggcttatatt tgtgatatct tttatgacag atatatttgc atattttagt ggttatttac
    16381 ttggaaaaca taagctaatt cctaaagtta gtcctaaaaa gactatagaa ggagctattg
    16441 gaggaataat aggaagtaca ctttgttgta ttttatttgg atatttattt ggcattgatt
    16501 tgcttcaatt agcgataata ggaagtatag gaagtgtaat agctcaatta ggagatttat
    16561 ttgcatcttc tataaagaga tatgtaggga taaaggatta tggaaagata atacctggtc
    16621 atgggggtat attagataga tttgatagtg ttatattagt tgcacctttt gtatatagtg
    16681 cgattaaatt ttttattaga taaagattgg ttggatttaa aagagcaatc tataattgct
    16741 agtaatgaaa atagctatgt aacttttaaa tgcagagata ttctgcagtt attagttaca
    16801 tggctatttt cattgttcaa aaatttagaa gaccttgggg aattgtataa gaataggtat
//
LOCUS       DAELLV010000064        16531 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.064, whole genome shotgun sequence.
ACCESSION   DAELLV010000064 DAELLV010000000
VERSION     DAELLV010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16531
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.064"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            110..778
                     /locus_tag="KPD58_001695"
     CDS             110..778
                     /locus_tag="KPD58_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265467.1"
                     /translation="MSKLESTTEKVEVKWYGYVALILGALFFSGIFKDAPGPLKVLDF
                     NNVLGSFGTLGTINDGVGTLAANFRGDGGTGPRDGWLYALTLIPSVMFALGVVRVIDH
                     LDGMKAAQKLLSPLLKPLLGLPGFAGLTLIASLQSTDAAASMTKELKDDGYIDEKQKA
                     VFCAFQFSGASAITNFFASGAALFPFIGDVPIFIPLALILIMKFVGANLLRLYLNKFD
                     KGEA"
     gene            780..1265
                     /locus_tag="KPD58_001696"
     CDS             780..1265
                     /locus_tag="KPD58_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBG0265468.1"
                     /translation="MSEVAKKEVKVKKGNVIDAFIGGARNGFQISTNSMAPNVIFGFA
                     IISVFNLTGLLDVIGTIFTPVMSIFGLPGVAATAIMTIFISMGGASGVIAGLFTAGQI
                     SQAHVAILLPMLLLSGAQIQFMGRLLGTAELKTKYYPHMFVIATLNGCIAMLIMNYFV
                     V"
     gene            1326..2447
                     /locus_tag="KPD58_001697"
     CDS             1326..2447
                     /locus_tag="KPD58_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HBG0265469.1"
                     /translation="METNKDYIEYVHKVYDYLHTIPEKGFEEYKTSEFVLKELEKMGF
                     ENINKGIAGTGIIVTIDSKKEGPVFAIRADMDALEFIIDGEKKMIHACGHDGHTSMLL
                     TAAKLIKERNLVKRGKLKILFQPGEEKLYGALRVIDSGLLDDVEEMVGMHLRPGQDCR
                     LGKAIPAMYHCASCIMEFTIKGITCHGSKPHLGVNAVDAVCLAVNAINAIRVNPAIPN
                     SIKVTNISVKGETYNNIPDEAFMALDIRAESNDIIEELKEKAIHAVKTAVSSINAEAV
                     LISKDGVPAAEFDEELVNLAEETIKDVLGADASIGIFKNSGGEDFHYYTQKLKCKTTY
                     IGLGADATPGFHNPNVTFDTKALEYGVDIWCRLVEKRLG"
     gene            2728..3918
                     /locus_tag="KPD58_001698"
     CDS             2728..3918
                     /locus_tag="KPD58_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG0265470.1"
                     /translation="MSKYNFDRVIDRVGTDCVKWDFRTNCSTKAQKDGLPFWIADMDF
                     ECAEPIIEALRKKVEHKIFGYSSNDSDKYFNAVCDWYKRRFNWEINRKDIIFSPGVVP
                     AVAILVRILTNSNEGVIVQKPVYYPFEAKIKSNNRDVVNNPLIYENGTYRMDYDDLEE
                     KAKCSNNKVLILCSPHNPVGRVWREDELKKVVEICKKYDLWIIADEIHSDLIRKGFKH
                     TPLQSLCPEYKDKIVTCIAPSKTFNLAGMQLSNIIVNNDELKKKYQEEVTAVGVATSP
                     NPFAIVATIAAYNESEDWLNELNDYLDNNIQFIDEYLKENLPKVKLVYPEGTYLAWLD
                     FTAYGLNEVELEDLMFKKANVLFDEGYIFGKEGIGFERINVACPQSLLKECMDRLKTI
                     FENL"
     misc_binding    4052..4307
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4366..5367
                     /locus_tag="KPD58_001699"
     CDS             4366..5367
                     /locus_tag="KPD58_001699"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HBG0265471.1"
                     /translation="MSTIIPKDYKSSLNVIDTQIAIKKLKDFFEMRLSNELNLIRVSS
                     PLFVLPETGANDNLNGEKAVSFDIPFMNKNAEIVQSLAKWKRLALKKYGFEVGSGLYT
                     DMNAIRKNEELDNIHSLYVDQWDWELVIDKSSRNEKTLKDVVKRLYGVFKDTEIFVCS
                     MYEGIKEILPEEITFITSQELEDMYPELSPKEREDKIVKEKKAVFITQIGKTLISGEK
                     HDGRSPDYDDWELNGDILFYNPVLDSALELSSMGIRVDEESLDRQLKLAGCEERKEFD
                     YHKMLLNGELPYTIGGGIGQSRICMYFLQKAHIGEVQVGVWPQDMIQNCSSAGIELL"
     gene            5471..5860
                     /locus_tag="KPD58_001700"
     CDS             5471..5860
                     /locus_tag="KPD58_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HBG0265472.1"
                     /translation="MEFLKIIINIVLDILKKILVRFKNAKFGLVFVFDLLKLPDFMTD
                     KRINIVDKIKVISVLIFTISYFVSGVDIIPEMIAGAFGFIDDAIVLIWSIGIVNEEIN
                     KYRVIAKKDKHSNIIENVEFSIKDEEE"
     gene            6021..7229
                     /locus_tag="KPD58_001701"
     CDS             6021..7229
                     /locus_tag="KPD58_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HBG0265473.1"
                     /translation="MKKKIGLVPKLIIGIIVGILIGSYAPEIIVQILVTVSTLFSAFL
                     KFVIPFIIIGFVTAGIADLATGAGKLLGITTGIAYGSTLIAGLLSFAVSTLIFPNFID
                     ASVASQIGDPEAGMLAPIFTIPLEPMVDVTAAIVFAFVMGLGISALRNHGKGETLFNF
                     FQEFQGIVTKTLSTVIIPLLPLYIAGTFANITIAGEVWTILGVFWKVYLVVIPLHFVY
                     MACQFTAAGVFSGKNPVRMLKNQVPGYLTAIGTQSSAATIPVNVGCAEKNGVSKQIRE
                     FVVPLCATIHLSGSMISITGFTTAVLMMNGMPHGIGVLFPYIAMLGIAMVAAPGAPGG
                     AIMSALPFLPMVGIPSDGGLASLMIALYLTQDSFGTACNVSGDNAIAVVVDKINDGMS
                     KKGNKEEQSA"
     gene            complement(7315..8391)
                     /locus_tag="KPD58_001702"
     CDS             complement(7315..8391)
                     /locus_tag="KPD58_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG0265474.1"
                     /translation="MSTNHGANLYSLSSKYGFSKEEFMDFSSNINPFGTSSLAKQYIV
                     NNIDMVSMYPDPDYIDLKTSISNYCKCSIDNIVLGSGATELISSFIHTINPKQALLLS
                     PAYSEYEKELSKINCSIEKYFAKEEDNFHINLENLIKTINAKDYDLVVICNPSNPTGF
                     AFTKVEVREILKNTDSFLMIDETYVEFTDTDTYSCTQLVDDYSNLFVIRGTSKFFSTP
                     GIRLGYGLISNTNVKNEINKNLDLWNINIIASKMGEIMFSDLDFISNTISLMNTERDY
                     LLKELKNIKSLDIYNTKGNFILCKIKTKELTAKSLREQLLPQKIIIRDCCSFEGLDEY
                     FFRVCILKPNENKLLISSLKAIFK"
     gene            8620..9147
                     /gene="hpt"
                     /locus_tag="KPD58_001703"
     CDS             8620..9147
                     /gene="hpt"
                     /locus_tag="KPD58_001703"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBG0265475.1"
                     /translation="MYKVTGKMLTEEQIKEKVYELGKKIEEDFKGEDLLVVGILKGAS
                     VFVSDLIRCIDLDVNIDFMSVTSYGNSTESSGTVKILKDLDVDIEGKNVLIVEDIIDS
                     GLTLSNLVAALKTRNPKSLKLCTLLDKPQRRKANIPVDYVGFVIEDKFIVGYGIDYAE
                     KYRNLPYIGIVEDVE"
     gene            9236..10168
                     /locus_tag="KPD58_001704"
     CDS             9236..10168
                     /locus_tag="KPD58_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBG0265476.1"
                     /translation="MDIQLNILLFFQNLRNPILNFIFLVFTISTEAPLLILITTIIYW
                     CINKKCGQKILFAIIGNFVVNLGIKEFVKAPRPIGIKGLESLRVSTAGGYSFPSAHTQ
                     TATTFWVSIMTIFKKRYLHLIGAVMVLGVGLSRLYLAVHWPIDVIAGWILGIFFTVVF
                     VKIFDYIDDNKNYILLVVLLIPFIIVAIFLNSPEYFEKFGIIVGFVFGYMVEDRFVKF
                     NTDNNNKKINFSNKNKGSKNIIFSRICRFLVGIVTIGMLYIGIKLFISMLIVTLNISD
                     STMSIIFMNFIKNTVVVFYGIAGAPALFKLLKLN"
     gene            10230..11456
                     /locus_tag="KPD58_001705"
     CDS             10230..11456
                     /locus_tag="KPD58_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG0265477.1"
                     /translation="MKKVVIIGGGPAGMIAASTACEKGYDVTLIEKNHKLGKKLAITG
                     KGRCNITNACEIEELIENVPTNGKFLYSAFYTFTNDDVISMFNNLGVKTKTERGKRVF
                     PESDKAFDIVNALERQLKSKKVNILLNSKVEKIISKNNKIEKVILNDKKEIKCDSVVV
                     ATGGLSYPLTGSTGDGYKFAISQGHTIIDTKPSLIGIEVQESFTKDLEKLSLRNVEIR
                     VFNSKQKKVYSDFGELEFTKFGLDGPIIKSASCRMKDTRKENYTILLDLKPALDEEKL
                     DKRVQKDFQKYTNKKFEKALDDLLPKKLIPIIINLSEINPNTVVHQISREQRKNLVHL
                     LKNLKFTVKRYRPIEEAIITSGGVKVNEINSSTMESKLVEGLFFAGEVIDIDAYTGGF
                     NLQIAFSTGYLAGFNC"
     gene            11540..11761
                     /locus_tag="KPD58_001706"
     CDS             11540..11761
                     /locus_tag="KPD58_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HBG0265478.1"
                     /translation="MASNNNNNRTVVPGAKEALNQMKLEIANELGMSNYQQVDKGNLT
                     ARENGYVGGYMTKKLVEMAEQQMAGKSNR"
     gene            11950..12120
                     /locus_tag="KPD58_001707"
     CDS             11950..12120
                     /locus_tag="KPD58_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265479.1"
                     /translation="MSSVNFEDLKNKFINSDLDEKIRIYTTTEGLSVEQFKELLKYYP
                     IQHLSKLEKALG"
     gene            complement(12213..12674)
                     /locus_tag="KPD58_001708"
     CDS             complement(12213..12674)
                     /locus_tag="KPD58_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265480.1"
                     /translation="MQIEQLDLETRNKIYCYTKKILRKYQKGITSGKLTADKFADNIL
                     SQDFISSILNEKIVNETNFKISYRNYIETLINIQNENLSNLRKKSTKTAKCFNISQIT
                     QLKNLLSNTGYNLLIPYKYLTARDIEGIVTLINTGSIELGNERIYNYISKA"
     gene            12857..13324
                     /locus_tag="KPD58_001709"
     CDS             12857..13324
                     /locus_tag="KPD58_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265481.1"
                     /translation="MKMLNGIIKLFYSEIVKTKINNLDSSGYSLRDECKEIRNITLKI
                     DSVFFKLLKLAFGSILLMVAFNLNMVLGIGVFLVGVVYILYKRNMEEQVKEHINNVKN
                     NIDISKITFMDEKGRTGLNALITLLLIGLLTGFNWILVASFVVVFVFTIKNVC"
     gene            13367..15130
                     /locus_tag="KPD58_001710"
     CDS             13367..15130
                     /locus_tag="KPD58_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HBG0265482.1"
                     /translation="MTNGSYKMKNLVEISAMVTESTDFFNIKDDIIEKMLEVVHPTKA
                     CVNLFYKNDSKYAYLVCSQTLEYVPQIFPINSLKGAKIDFDTYPEYIHEAVNEKKIIY
                     VENVFEDSRAEGERDLAKAEGYIGRIVFPFIINNVVVGFMTCFLKEDDYITEQDIDFI
                     SSVASLLSLSIEITNKNNNVQILIDKLRGSISAINEATKKLYLNKSIDGFLENLSKQA
                     CNITNSKEALIIINSHHNKDAIVSCYNVEQKKKTNLYPMIDMFLKKESLGGYLNNLKP
                     CSKKGINLESYIYYKLQDKNDTIGCIVCANSKNYTSDDLNILSILSKQVSVGMQLYEH
                     NEKEVKHKVLENELNILNKQQQLIMDKGKMDCNDEKELYFYHKPARVVGGDFYYATKV
                     DDEHIVYIVADVMGHGMVSNYVVAMIKGAFKVLCNQYNTPREIMTNLNRMLYDEFDKM
                     GVFTTCLIGIIDTKENMITVSNAGHYSPVVVKKDGTIENNLNCKKGIPIGIMEDATYE
                     NNTLSMEDYAMVCMYTDGILEIKNSSKEEYGINRLENFLKENFRLNQQLIVENLKLDL
                     KNFSSKDNYDDDILIVMLKDR"
     gene            15138..15746
                     /locus_tag="KPD58_001711"
     CDS             15138..15746
                     /locus_tag="KPD58_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG0265483.1"
                     /translation="MDSNDKKGLKSPEIIIINGSPRINKNCSFIANEVKAMLEECGLS
                     CKIFNIYNMDIEYCTACGFCEKTGYCKFNDDMKPLYKMFDDSKGTIVISPVHFDSISA
                     KLKTVVDRTQAIYASKYVLNKPSIDRSKKRIGMYIAVGGSKSYDSQFKGGQIVMDFFF
                     KSINTKLLHNIYLNNTDDVSYIDNQEFKDIIYKNLKIYIDSI"
     gene            15818..16387
                     /locus_tag="KPD58_001712"
     CDS             15818..16387
                     /locus_tag="KPD58_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HBG0265484.1"
                     /translation="MKLPIALSNKHVHLSQADIDVLFGAGHELTKFKPLSQPGQYACE
                     EKVDLVGPKGTLKGVRVLGPARAHTQVEISLADGFPLGVKAPIKESGDIAGTPGVKLV
                     GPAGEVEMKEGVIVASRHIHMSNEDAAKFGVTDKQIVKVKTSGPRALVFENVLVRASD
                     SFNLEMHVDMEEGNAAGVKNGDLVELIVD"
BASE COUNT         6463 a         1678 c         2920 g         5470 t
ORIGIN      
        1 tacaataaaa aaataaatac aaaggcttta tcattaaggt attggcataa gttttgctta
       61 ttaaacaagt atactaataa aattcattta aacaggaggg agaatttata tgagtaaatt
      121 agaatcaaca acagaaaaag ttgaggtaaa atggtatggg tatgtagcat taatattggg
      181 agcattattt ttttcaggaa tatttaaaga tgcaccagga ccattaaaag tattggattt
      241 taacaatgta ttaggttctt ttggaacatt gggaacaatt aatgatggtg taggtacatt
      301 agctgcaaac tttagaggtg atggaggtac tggccctcga gatggatggc tatatgcatt
      361 gacattaata ccatcagtta tgtttgcact tggagtagtt agagttatag accacttaga
      421 tggaatgaag gcagctcaaa agcttcttag tccactatta aagccattat tagggttacc
      481 tggatttgca ggactaactt tgatagctag tttacagtct acagatgcag ctgcatctat
      541 gactaaagaa cttaaagatg atgggtatat agatgaaaaa caaaaagctg tattttgtgc
      601 atttcaattt tcaggtgcgt ctgcaattac gaatttcttt gcttctggag cagcactatt
      661 tccatttata ggagatgtac caatatttat accacttgct ttaatattaa ttatgaagtt
      721 tgttggggcg aacttactta gattatattt aaataaattt gacaaggggg aagcttaaca
      781 tgagtgaggt agcaaaaaaa gaagtaaaag ttaaaaaagg aaatgtgata gatgcgttta
      841 ttggaggagc tagaaatgga ttccaaataa gtacaaattc aatggctcca aatgtcatct
      901 tcggttttgc tataatttct gtgttcaatt taacaggtct tttggatgta atagggacaa
      961 tatttacacc tgtaatgagt atctttggac ttccaggtgt tgcagcaaca gctataatga
     1021 caatatttat atcaatggga ggagcatctg gagtaatcgc aggtttattt acagcaggtc
     1081 aaataagtca agcacatgtt gcaattctat tgccaatgtt attgctttca ggagcgcaga
     1141 tacaatttat ggggagactt ttaggaacag ctgagttaaa aactaaatat tatccacata
     1201 tgtttgttat agcaacttta aatggttgta ttgcaatgct tataatgaat tatttcgtag
     1261 tataggtaat tataataaag atttaatata aatggaaagt ttaaatttta aggaggagct
     1321 agattatgga aactaacaaa gactatattg agtatgtaca taaagtgtat gattatctac
     1381 acactatacc agaaaaagga tttgaagaat ataagacatc agagtttgtt ttaaaagagc
     1441 ttgaaaaaat ggggtttgaa aatattaata aaggaattgc aggaactggt attatagtaa
     1501 ctatagattc taaaaaagaa ggaccagtat ttgctataag agcagatatg gatgcattgg
     1561 aatttatcat agatggagaa aagaaaatga tacatgcttg tggtcatgat ggtcatactt
     1621 ctatgctttt aacagctgca aagttaataa aagagagaaa tttagtaaaa agaggtaagt
     1681 taaaaatact ttttcaacca ggtgaagaaa aattatatgg agcattaaga gtaattgatt
     1741 ctggtttgtt ggatgatgta gaagaaatgg tagggatgca tttaagacct ggacaagatt
     1801 gtaggttggg aaaggcgata cctgcaatgt accattgtgc tagctgtata atggaattta
     1861 cgattaaagg tatcacttgt catggttcta aaccacattt aggagtaaat gcagtagatg
     1921 ctgtctgttt agcagtgaat gcaataaatg caataagagt aaatccagct ataccaaatt
     1981 ctataaaagt aacaaatata tcagttaagg gagaaactta taataatata ccagatgaag
     2041 catttatggc tttagatata agggcagaaa gtaatgatat aatagaagaa ttaaaagaaa
     2101 aagcaataca tgctgtaaaa actgctgtaa gttctataaa tgcagaagct gtactaattt
     2161 ccaaagatgg agtaccagca gcagaatttg atgaagaact tgtaaacttg gctgaagaaa
     2221 ctataaaaga tgtattggga gctgatgcat ctataggtat atttaaaaat tctggtggag
     2281 aagatttcca ttattataca caaaaactaa aatgtaaaac tacatatatt ggattaggag
     2341 ctgatgctac accaggattt cataatccaa atgtaacatt tgatacgaaa gctttagaat
     2401 atggtgtaga tatatggtgt agattggttg aaaagagatt gggataacat ccaaactaat
     2461 aattctacat ttaaaattat aattatagaa gttatagaaa aagattgctt atgttaatta
     2521 gaaaataatt aatatgagca atcttatttt gatataatga aactgatttt ggaatattga
     2581 aaaaatatga tggaaaagta attatatttt aaatctattg gacaaaaaag tgatattatt
     2641 ataatattaa gaattgtaaa ataataccaa agtgcatcta gtaatagact taacaattat
     2701 aaaaatttta ttcttaggag ggaaaaaatg agtaagtata attttgacag agtaatagat
     2761 agagtaggaa cagattgtgt aaaatgggat tttagaacta attgttcaac aaaagcacaa
     2821 aaagatggtt tgcctttttg gattgctgat atggattttg aatgtgcaga accaataata
     2881 gaagcattac gtaaaaaagt tgaacataaa atatttggat atagttcaaa tgatagtgat
     2941 aaatacttta atgcagtttg tgattggtat aaaaggagat ttaactggga aataaataga
     3001 aaagatatta tttttagtcc tggtgttgta cctgctgttg ctattttagt aagaatatta
     3061 actaattcta atgaaggtgt aatagtccaa aaaccagtgt attacccatt tgaagctaag
     3121 ataaagagca ataataggga tgttgtaaac aatcctctga tatatgagaa tggtacttat
     3181 aggatggatt atgatgattt agaagaaaaa gctaagtgta gcaacaataa agtactgata
     3241 ctttgtagtc ctcacaaccc tgttggaaga gtttggagag aagatgaatt aaaaaaggtt
     3301 gtagaaatat gtaaaaaata tgacctatgg ataatagctg atgagataca ttctgattta
     3361 attagaaaag gatttaaaca tactccttta cagtcattat gcccagagta caaggataaa
     3421 atagttacat gtatagctcc aagtaaaaca tttaatctgg caggaatgca gttgtctaat
     3481 ataatagtga ataatgatga actaaaaaag aaatatcaag aagaggttac agctgttgga
     3541 gttgcaactt caccaaatcc ctttgctatt gtagctacaa tagcagcata taatgaaagt
     3601 gaagactggc ttaatgaatt aaatgattac ttagataata atatacaatt tattgatgaa
     3661 tacttaaaag aaaacctacc aaaagtaaaa cttgtttatc cagaaggaac atatctagca
     3721 tggttagatt ttactgctta tggattaaat gaagtagaac ttgaagattt aatgtttaaa
     3781 aaagctaatg tattatttga tgaaggttat atatttggaa aagaaggaat aggatttgaa
     3841 agaataaatg tagcttgtcc acagtcttta ttaaaagaat gtatggacag attaaagact
     3901 atatttgaaa atttataaag tataggtaaa aatttataaa gtgtttaaaa ataaaaaaat
     3961 aataaacttg acaaatttta actaatcact tatcatacta ttatgaataa atagaaaaca
     4021 taagagagct aaattttatt gaattaaact cttaaagact ttgaaaggaa gagtagttat
     4081 agaatgtaat taaagcgagt tggtgatggt gtgagaccaa tatgaagtct ataatgaaga
     4141 acatcctgga gttgctaacc gaaattttaa aagagtaggc ttagtcggaa ccaaaccgtt
     4201 atcatattgg ggtgtatcga gtaatatctg tacattgtga gctgggtttg aatttatttc
     4261 aaactaatca gggtggtacc gcggaaaagc aacctttcgt cccttttata tagggatgaa
     4321 gggttttttt aatggaaaaa tatataaata tagggagtga atgatatgag cacaataata
     4381 ccaaaggatt ataaaagtag tttgaacgta attgatacac aaatagctat aaaaaaatta
     4441 aaagattttt ttgaaatgag actaagtaat gaattaaatc ttataagagt ttcttctccg
     4501 ttatttgtat tgccagaaac aggtgcaaat gataatttaa atggagaaaa ggctgtaagc
     4561 tttgatatac catttatgaa taaaaatgca gaaattgttc aatcacttgc aaaatggaaa
     4621 agattagccc ttaaaaaata tgggtttgaa gttggtagtg gtttatatac agatatgaat
     4681 gcaataagaa aaaatgaaga attagacaat atacattctc tttatgtaga ccaatgggat
     4741 tgggaattgg ttatagataa aagtagtaga aatgaaaaga ctctaaaaga tgttgtaaaa
     4801 agattgtatg gagtatttaa agatactgaa atttttgtat gtagtatgta tgaaggtata
     4861 aaagaaatat taccagaaga aataactttt ataacatcac aagagttaga agatatgtat
     4921 ccagaattat caccaaagga aagagaagat aaaattgtaa aagagaagaa agcagtattt
     4981 ataactcaaa taggaaaaac attaatatca ggagaaaaac atgatggaag aagccctgat
     5041 tatgatgatt gggaattgaa tggggatata ttattttaca atcctgtttt agattcagca
     5101 ttagaattat cttcaatggg aattagagtt gatgaagaat ctcttgatag acaattgaaa
     5161 cttgctggat gtgaagagag aaaagagttt gactatcata agatgctttt aaatggcgaa
     5221 cttccgtata caattggtgg aggaattggt cagtcaagaa tatgtatgta tttcttacaa
     5281 aaagctcata taggagaagt acaagttgga gtatggccac aggatatgat tcaaaattgt
     5341 agtagtgcag gaattgaatt attataattt aaatataaac taattgataa aatttatatc
     5401 taatatgttc atcaaattaa ttatatcggt tataatgtag aataacctat aattataaga
     5461 ggtgataaat atggagtttt taaaaataat cattaatata gttttagata ttttaaaaaa
     5521 aattttggtc agatttaaaa atgctaaatt tggtttggtt tttgtatttg atttacttaa
     5581 actacctgat tttatgacag ataaaagaat caatatagtt gataaaataa aggtaatttc
     5641 tgtattaatt tttactatat cctactttgt atctggggtt gatataattc cagagatgat
     5701 tgcaggggca tttggtttta tagatgatgc tatagtttta atttggagta taggtatagt
     5761 gaatgaagaa attaataagt atagagttat cgcaaaaaaa gataaacatt caaatataat
     5821 tgaaaatgta gaattttcta taaaagatga agaagaataa caatttatat atgttattct
     5881 tttttttatt aacaaaaaat ggttaaattg ttgacggaga ggtaaaactc tattatcata
     5941 ataagtggat atccaatatt taactaagta ttcaatgtgc aagaatgctt aatttaaaaa
     6001 agttatggag ggtaaatatt atgaagaaaa aaataggttt agtgcccaaa ctaatcatcg
     6061 gtatcatagt cggtatttta atcggaagtt atgcaccaga aataatagta caaatattag
     6121 taacagttag tactttattc tcagcattcc taaaatttgt tataccattt ataattatag
     6181 gttttgtaac agcaggtata gctgatttag caacaggtgc aggaaagtta ttggggataa
     6241 ctacgggaat agcatatggt tctaccttaa tagcaggatt attatcattt gcagtatcta
     6301 cattaatctt cccaaatttt attgatgcaa gtgttgcaag tcaaattgga gacccagagg
     6361 caggaatgtt agctccaata ttcacaattc cattagagcc tatggtagat gtaacagctg
     6421 caatcgtatt tgcatttgta atgggacttg gaatatcagc tttaagaaat catggaaaag
     6481 gagagacttt atttaatttc ttccaagaat ttcaaggaat cgtaacgaaa acgttgtcaa
     6541 cagtgattat acctttatta cctttataca tagcaggaac ttttgcaaat ataactatag
     6601 caggggaagt atggacaata ttaggggtat tctggaaagt atacttagta gtaatacctt
     6661 tacattttgt atatatggca tgtcaattca cagcagcagg tgtattttca ggaaaaaatc
     6721 cagtaagaat gttaaaaaat caagtacctg gatatttaac agctatagga actcaatctt
     6781 cagcagcaac tataccagtt aatgttggtt gtgcagaaaa aaatggagta agtaagcaaa
     6841 taagagaatt tgttgtacct ctttgtgcaa caatacattt atcaggtagt atgatatcaa
     6901 taacaggatt tacaactgca gttttaatga tgaatggtat gccacatgga ataggtgtat
     6961 tattccctta tattgcaatg ttaggtatag caatggttgc agcaccagga gcaccaggtg
     7021 gggctataat gagtgcactt cctttcctac caatggttgg aataccatca gatggtgggc
     7081 ttgcaagttt aatgatagct ttatacttaa ctcaagacag ttttggtaca gcatgtaatg
     7141 tatcaggaga taatgcaata gcagtagtag tagataaaat aaatgatgga atgagtaaaa
     7201 aaggaaataa agaagagcaa agtgcttagt tttttaataa tcaaaaatga gttatctcaa
     7261 atagagatag ctcatttttt atggtataaa aaattataaa ataaagtgat ttttttattt
     7321 aaaaattgct ttaagagatg atattaataa tttattttca ttaggtttta atatacatac
     7381 tctaaagaaa tactcatcta gaccttcaaa tgaacaacaa tctcttatta ttattttttg
     7441 tggaagcaat tgttctcgaa gagatttagc agtcaattcc tttgtcttga ttttacacaa
     7501 tatgaaattg cctttagtat tgtatatatc taagcttttt atattcttaa gctctttaag
     7561 caggtaatct ctttcagtgt tcattaagga aatagtattt gatataaaat ctaaatcact
     7621 aaacatgatt tcacccattt tagaagctat tatgtttata ttccataaat ctagattttt
     7681 atttatttca tttttgacat tagtgttaga tataagacca tatccaagcc ttattccagg
     7741 cgttgaaaaa aattttgatg tacctcttat aacgaataaa ttagaataat catcaactaa
     7801 ttgagtacaa gagtatgtat ctgtatcagt aaactctaca taagtctcat caatcattaa
     7861 aaatgagtct gtatttttta aaatttctct cacttctact ttagtaaatg caaatccagt
     7921 tggattacta ggattacaga ttactaccaa atcataatct ttagcattga tagtttttat
     7981 aagattttct aaatttatat gaaaattatc ttcttctttt gcaaagtatt tttctataga
     8041 acaattaatt ttagataact ctttttcata ctctgaatag gctggagaga gtaaaagagc
     8101 ctgtttaggg ttaattgtat gtataaatga tgaaattagt tctgtagctc cactgccaag
     8161 tactatatta tctatagaac atttgcagta attggatata gaagttttta agtctatgta
     8221 atcagggtca ggatacatag ataccatatc tatattgttt acaatgtatt gtttggctaa
     8281 acttgatgta ccaaaaggat ttatatttga gctaaaatcc ataaattctt cctttgaaaa
     8341 gccatactta ctagataaac tatataaatt agctccatgg tttgtgctca taaaagtacc
     8401 tccaaaaata aaattatata atcgataaaa ataactgatt aatatatgta catatagatt
     8461 ataatacaaa ggaatcgtta ttccaaattt ttaatggaaa aagcaaaaaa gtatggataa
     8521 gctgttaatt taggtttaaa aatgatgaaa aagattttaa ttgatatttt ggtatgctat
     8581 aatatataag ttaatagaag gaaagcggga ggatacaaaa tgtacaaagt tacaggaaaa
     8641 atgttgacag aagaacaaat aaaagaaaaa gtttatgaat taggaaaaaa gatagaagaa
     8701 gactttaaag gcgaagattt actagtggta ggtatactta aaggagctag tgtatttgtt
     8761 tcagatttaa taagatgtat agatttggat gtaaatatag attttatgag tgttacaagt
     8821 tatggaaact caacagagtc ttctggaaca gttaaaatat taaaagattt agatgtagat
     8881 attgaaggaa agaatgtgtt gattgtagag gatataatag attctggttt aacattaagt
     8941 aatctagttg cagctttaaa aactagaaat ccaaaatctt taaaactatg tactctactt
     9001 gacaaacctc agagaagaaa agctaatata cctgtagact atgttggatt tgttatagaa
     9061 gacaaattta ttgttggtta tggaatagac tatgctgaaa agtatagaaa tttaccttat
     9121 ataggtatag ttgaggatgt agaatagatt taaatgtgaa tcaaaagcac taatttctaa
     9181 gggagttagt gctttttagt taattatata aaattttagt aaaggagaca tctatatgga
     9241 tattcagtta aatatattat tatttttcca aaatttaaga aatccaattt tgaactttat
     9301 atttcttgtc tttacaataa gcacagaagc accgttatta atattaatta caacaattat
     9361 atactggtgt ataaataaaa aatgtggaca gaaaatactg tttgctataa ttggaaattt
     9421 tgttgtaaat ttaggcatca aagagtttgt aaaagctccc agaccaatag gaataaaagg
     9481 tcttgaatca ttgagagttt ctactgctgg tggttattct tttccaagtg ctcatactca
     9541 aactgcaaca acattttggg ttagtattat gactatattt aaaaagagat atcttcattt
     9601 aataggtgca gttatggttt taggagtagg attgtctagg ctgtatttag ctgttcattg
     9661 gccaatagat gtgatagcgg gttggatatt aggaatattt tttacagttg tatttgtaaa
     9721 aatatttgac tatattgatg ataataaaaa ttatatttta cttgtagttt tacttatacc
     9781 atttataata gtggctatat ttctaaatag ccctgaatat tttgaaaaat ttggtataat
     9841 agtaggattt gtatttggat atatggttga ggatagattt gttaaattta atacagataa
     9901 taataacaaa aaaataaatt ttagtaataa aaataagggg agtaaaaata taattttttc
     9961 aagaatatgt aggtttttgg ttgggatagt aacaatagga atgttatata ttggcattaa
    10021 gttgtttatt tcaatgttaa tagtcacttt aaatataagt gactctacta tgagtataat
    10081 ttttatgaat tttattaaga atacagtagt tgttttttat ggaatagcag gagctccagc
    10141 attatttaaa cttttaaaat taaattgatt ttaaggtata gagtcaatag tttaaattaa
    10201 aaaaattaag ggtagaaaag agtgattatt tgaagaaggt agtaataata ggtggtggac
    10261 cagcagggat gatagccgca tcaactgcat gtgagaaagg ctatgatgta acattaattg
    10321 agaaaaatca taaattagga aaaaaacttg caataacagg aaaaggtaga tgtaatatta
    10381 ctaacgcttg tgaaatagag gaattaatag aaaatgtacc tacaaatgga aaatttcttt
    10441 atagtgcttt ttatactttt acaaatgatg atgtaataag tatgtttaat aatcttggag
    10501 taaagactaa aacagaaaga ggaaaaaggg tttttccaga gagtgataaa gcatttgata
    10561 ttgtaaatgc tcttgaaaga caacttaaaa gtaagaaagt aaatatatta ttaaattcga
    10621 aagtagaaaa aataatatct aaaaataata aaattgaaaa agtaatttta aacgataaaa
    10681 aagaaattaa gtgtgattca gttgtagtag caactggtgg attaagttat ccacttactg
    10741 gttctactgg tgatggatat aaatttgcaa tttcacaggg gcacacaatt atagatacaa
    10801 aaccatcttt aattggtata gaagtacaag aaagttttac aaaagattta gaaaaattat
    10861 cattgagaaa tgttgaaata agggtgttta atagcaaaca aaagaaagtt tatagtgatt
    10921 ttggagaact tgaatttact aaatttggtt tagatggacc tataatcaag tctgcaagtt
    10981 gtagaatgaa agatacaagg aaagaaaatt acactatatt attagattta aaacctgcat
    11041 tagatgagga aaaattggat aaaagagttc aaaaagattt tcagaagtat acaaataaaa
    11101 aatttgaaaa agcattagat gatttacttc caaaaaaact aatacctata ataataaact
    11161 taagtgaaat taatccaaat acggttgttc accaaatttc tagagagcag agaaaaaact
    11221 tagtccattt attaaaaaat ttaaagttta cagtaaagag atacagacct attgaagaag
    11281 ctataataac ttctggtggt gtaaaagtaa acgaaataaa ttcaagtaca atggaatcaa
    11341 aattagtaga aggtttattt tttgctggag aagtaataga tatagatgca tatacaggtg
    11401 gattcaattt gcaaatagct ttttctactg gatatttggc aggttttaat tgctaaaaat
    11461 tacctgaatt aaaaatgttt aaaatcacat aatattatca gcaacacaaa ataaattaaa
    11521 cagaaggaag gtaatcaaca tggcaagtaa caataacaac aacagaacag tagtaccagg
    11581 agcaaaagag gctttaaacc aaatgaaatt agaaatagct aatgaacttg gtatgagtaa
    11641 ttatcaacaa gttgataaag gaaaccttac tgcaagagaa aatggttatg taggtggata
    11701 catgactaaa aaattagttg aaatggctga acaacaaatg gctggaaaaa gcaacagata
    11761 gttataaata attatttata actattggct attagtttag ctagagctat ctcaaatata
    11821 gaaagtaatt tctaaattga gatagctttt ttttattaaa ttattaaata gatatatttt
    11881 tatgtaataa attttatgta tttggataaa ataatacaaa tgaatatgtt acataaggag
    11941 tgatgtaaaa tgagtagtgt aaattttgag gatttaaaaa acaaatttat taatagtgac
    12001 ttagatgaaa aaataagaat atatacaaca acagaaggtt tatcagtaga acaatttaaa
    12061 gagctactaa aatattatcc aatacaacat ttatctaaac tagaaaaagc acttggatag
    12121 cagtatttaa tccaaaatta aaaaggcaat ttatttaata ttaaaaatat tttttattaa
    12181 ataaattgcc tttttataat aggtttaaat tattatgctt ttgatatata attatagatt
    12241 ctctcatttc caagttcaat agacccagta ttaataagag taactattcc ttctatatct
    12301 ctagcagtta agtatttata tggaattaat aaattataac cagtattact tagtaagttt
    12361 tttaattggg ttatttgaga gatattgaaa cattttgcag ttttagtaga tttttttctt
    12421 aagttagata aattttcatt ttgaatattt attaaagttt ctatatagtt tctataagat
    12481 atcttaaagt ttgtctcatt gactattttt tcattaagta tactactgat aaaatcttga
    12541 gacaatatat tatctgcaaa tttatctgct gttaattttc cagatgttat gcctttttga
    12601 tattttctaa gaattttttt agtatagcag tatattttat ttctcgtttc taaatcgagt
    12661 tgttctattt gcacaaaacc acttccttct aattataaaa atatttatta aaaatctata
    12721 cttaactaat aaagcttcaa taacttattt acaataaata ctaaatcata aaatatttgt
    12781 tattttttat tattaccaag tataaaaaat attataattg taaaacttac aaatatatgt
    12841 aaaatattta ggagtgatga aaatgttaaa tgggataata aagctttttt attctgaaat
    12901 tgtaaaaaca aagattaata acttagattc aagtggatat tctttaagag atgaatgtaa
    12961 agaaatcaga aatataactc taaagataga tagtgtattt tttaagcttt taaagttagc
    13021 ttttggttca atattgctta tggtagcttt taatttaaac atggttttag gtataggagt
    13081 gtttttagtt ggtgtagttt atatactata taaaagaaat atggaagaac aagtaaaaga
    13141 acatataaat aatgtgaaaa ataatatcga catctctaaa ataactttta tggatgaaaa
    13201 agggagaaca ggtctaaatg cattaattac attacttctt ataggattat tgacaggatt
    13261 taactggata ttagttgcta gttttgtcgt tgtttttgtt tttactataa agaatgtatg
    13321 ttaaaatact aataaatagt ttaatatata gggggagaaa ttgagtatga ctaatggcag
    13381 ttataaaatg aagaatttag tggaaatatc cgctatggtg actgaatcta cagatttttt
    13441 taatataaaa gatgacataa tagaaaaaat gttagaagtt gttcacccta caaaggcatg
    13501 tgtgaatcta ttttataaaa atgattctaa atatgcctac ttagtatgtt cgcaaacctt
    13561 agaatatgta cctcaaattt ttccaataaa tagtcttaaa ggtgcaaaaa ttgattttga
    13621 cacatatcca gagtatatac atgaggctgt aaatgaaaaa aagataatat atgtggaaaa
    13681 tgtatttgaa gattctagag cagaaggtga acgagattta gcaaaagctg aaggttatat
    13741 tgggagaata gtttttcctt ttataataaa taacgtagtt gttggattta tgacttgttt
    13801 tttaaaagag gatgattata taacagaaca agatatagat tttatatcat ctgttgcatc
    13861 actcttaagt ttatcaatag aaataactaa taaaaataat aatgtacaaa ttttaattga
    13921 taaactaaga ggctcaataa gtgccataaa tgaagctact aaaaaactat accttaataa
    13981 gagtattgat ggctttttgg aaaatttaag taaacaagcg tgtaatatta ctaatagtaa
    14041 agaagcttta ataattataa atagtcatca taataaggat gctatagtta gttgttataa
    14101 tgttgagcaa aaaaagaaaa caaatttata tcctatgatt gatatgtttt taaagaaaga
    14161 gtcattgggg gggtatctaa ataatttaaa accatgctca aaaaagggaa taaatttgga
    14221 aagttatata tattacaaac ttcaggataa aaatgatact ataggatgta tagtttgtgc
    14281 aaatagcaaa aactatacta gtgatgattt aaatattctt agtatattgt ccaaacaagt
    14341 ttctgttggt atgcaattat atgaacataa tgaaaaggaa gtaaaacaca aagttcttga
    14401 aaacgagttg aacatattaa ataaacaaca acagcttatt atggataaag gtaaaatgga
    14461 ttgtaatgat gagaaagaac tttattttta ccataaacca gcaagggtag taggtggaga
    14521 tttttattat gctacaaaag tggatgatga acatatagtt tatattgttg ctgatgtaat
    14581 gggacatgga atggtatcaa attatgttgt tgcaatgata aaaggtgctt ttaaagtatt
    14641 atgtaatcaa tacaatacac caagagaaat aatgacaaat ttaaatagga tgttatatga
    14701 tgaatttgac aagatgggag tatttacaac ttgtttgata ggtattatag atactaaaga
    14761 aaatatgatt actgtgtcta atgcaggtca ctatagtcct gtagttgtaa agaaagatgg
    14821 cacaatagaa aataatttaa attgtaaaaa gggcatacct attggaataa tggaagatgc
    14881 tacttatgaa aataatactc tttcaatgga agattatgca atggtatgta tgtacactga
    14941 tggaatttta gagataaaaa attcatcaaa agaagagtat ggtataaacc gattagaaaa
    15001 ctttctaaaa gaaaatttta gattaaatca acagttaata gtagaaaatc taaaattgga
    15061 tttaaagaac ttctcatcaa aagataatta tgatgatgat attttaatag ttatgttaaa
    15121 ggataggtag attgattatg gattcaaacg ataaaaaagg acttaaaagt ccagagatta
    15181 taataataaa tggaagtcca agaattaata aaaattgcag ttttatagca aatgaagtaa
    15241 aagctatgct agaagagtgt ggattaagtt gtaaaatatt taatatctac aatatggata
    15301 ttgaatattg cacagcatgt ggattttgtg aaaaaacagg gtattgcaag tttaatgatg
    15361 atatgaagcc attatataaa atgtttgatg attcaaaagg aactatagta ataagtcctg
    15421 tacattttga ttcaatatca gctaaattaa agactgttgt tgatagaact caagctatat
    15481 atgcaagtaa atatgtatta aataaacctt caattgatag aagtaagaaa cggataggaa
    15541 tgtatattgc agtgggaggg agtaaatcat atgattcaca gtttaaaggt ggacagattg
    15601 ttatggattt tttctttaag agtataaata ctaaactttt acacaatata tatttaaaca
    15661 atacagatga tgtatcttat atagataatc aagaatttaa agatataata tataaaaatt
    15721 taaagatata tattgattca atataatttg attttgtagg taatattatg tataattgta
    15781 ttgcaataat ttaaataatt aaaaggaggg aatttcaatg aaattaccaa tagcattatc
    15841 taataaacat gttcatttga gtcaagctga tatagacgta ttatttggag caggtcatga
    15901 gttaactaaa tttaaaccat tatctcaacc agggcaatat gcctgtgaag aaaaagtgga
    15961 tttagtagga ccaaaaggaa ctttaaaagg agttagagta ctaggaccag ctagagctca
    16021 tactcaagtt gaaatatctt tagcagatgg tttcccatta ggagtaaaag caccaataaa
    16081 agaatctggt gatatagctg gaactccagg tgttaaatta gtaggacctg ctggagaagt
    16141 tgaaatgaaa gaaggagtta tagttgcttc tagacatata catatgagca atgaagatgc
    16201 tgctaaattt ggtgtaactg ataagcaaat agtaaaagtt aaaacatctg gaccaagagc
    16261 attagtattt gaaaatgtat tagtaagagc tagtgacagc tttaatctag aaatgcatgt
    16321 tgatatggaa gagggaaatg cagcaggcgt taaaaatgga gatttagtag aattaatagt
    16381 tgattaatat tataaataga ctttatgcta gtagatattt tatttgctag catattttta
    16441 aatgtaataa atttctatag gataaattta atcgattaga ttaatttata aaataaaggg
    16501 gtattataaa aaatgtaata aaaacttaca a
//
LOCUS       DAELLV010000065        16515 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.065, whole genome shotgun sequence.
ACCESSION   DAELLV010000065 DAELLV010000000
VERSION     DAELLV010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16515
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.065"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            223..2082
                     /locus_tag="KPD58_001713"
     CDS             223..2082
                     /locus_tag="KPD58_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG0265485.1"
                     /translation="MKRFLRRIILVLFILLIFISFISIKLIHNVGDYGKLINYVGIVR
                     GASQRLTKLEMNHQPNDELIEYIDEILQELITGHGDYGLVITDCNEYNEDLLLLEKKW
                     EDLNIEIKKVRMKEQNNQLLSISEEFFSLANDTVFKIENFSKEKSNYLMTLIIIISII
                     GILACIILILQYSKKMVRLEKLNVDLKNIAYKDELTGVNTIEKFKLDANQNICIHKDK
                     KFAVFYIDFENFKYINDIFGYDYGDMILKRYANLMMNDIGKYEIFAREIADRFVALRC
                     YVDKEDLVVRQRDVDNELINITNEIKNKHSITVVSGICCIEDVNEKLSIDGLINRANF
                     AQKTVKNKPGTNYAFYNDSIRKKMIEENTIKSRIHEAIEKREFIVYLQPKVNLHSQKI
                     NCAEALVRWLTPDKGIISPAIFIPVLEKNFFIALVDKYVFEEVCKWIRKRLDENKPFV
                     QISVNVSRIQFYNTKFVETYSNIQNKYRIPKNTIEIEFTESVAFENQKHLLEIIHDLH
                     ENGFTCSLDDFGKGYSSLSVLKDLPFDVLKLDSMFFKASLDKDKEKIVIKNIVHMLKE
                     LNITTVAEGIECKEQVEFLRDIGCDLVQGFVFYKPMPILEFEKILDKEFVYNS"
     gene            complement(2374..2973)
                     /locus_tag="KPD58_001714"
     CDS             complement(2374..2973)
                     /locus_tag="KPD58_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG0265486.1"
                     /translation="MAIELKKGQKINLTKKENSDLGEILVNLNWNQKVQKKGFFGSLR
                     SSNIDLDLGCLFEMKNGVKGAVQALGNAFGSLDNPPFAQLDGDDRTGSNTQGENLRIN
                     GNKIKDIKRILIYAFIYEGVANWSEADGIVTIKQKQDSDLVVKLDEHKNGYNMCSIAL
                     IENVNDETFSVEKVVKYFKGHREMDTEFHWGLKWVAGRK"
     gene            complement(3076..3873)
                     /locus_tag="KPD58_001715"
     CDS             complement(3076..3873)
                     /locus_tag="KPD58_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG0265487.1"
                     /translation="MKFKKLLCLLLCLVLTLAVVGCSKAKDDKKIVVGATLVPGGELL
                     EELKPLIKEKGYTLEVKNFDDYILPNEALNNGEIDANLFQHEPYLKEAVKAKGYKIMA
                     GKKLYVCPAILYSYKIKSVDEFKKGDTIAISNNPSSCSKNLRYLESIGLLTLPKGDGL
                     VSPKDIIENPKGIQFKELDIAQIPSSLPDVTAAFIDTTYAVPAGLDAKKNGIYTAPIN
                     DEYANLLAFRTEDKDSEKIKVLQDVLTSDKARSLIEEKYKGIVIPTF"
     regulatory      complement(3986..4092)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(4184..5170)
                     /locus_tag="KPD58_001716"
     CDS             complement(4184..5170)
                     /locus_tag="KPD58_001716"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBG0265488.1"
                     /translation="MLLIYNEKTNPYFNLAMEEYLLKNFDEDLFILWRNEPSIIVGKN
                     QNTLSEINLEYIKEYSIPVVRRQSGGGAVFHDLGNINFTFIACNNNNFSDFRRFTQPI
                     IDLLKTLDVNAEFSGRNDLLIDGKKFSGNAQYNYKNKVMHHGTLLFSSEINDLSKALK
                     VKPIKFEGKGIKSVKSRVTNISEHLHSKMDILEFKDAIMDYLSKTNTDNTNYCLSEHD
                     IKQIEKLTKSKYETWDWNFGNSPKYTLSNELKYSGGNVEFNLNVDKGIITSIKFFGDF
                     FGKCDVAFVENKLIGIRHEENALRNTLDSIEINDYFLGADTDILVSGILGVK"
     gene            5654..7384
                     /locus_tag="KPD58_001717"
     CDS             5654..7384
                     /locus_tag="KPD58_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HBG0265489.1"
                     /translation="MRNKKINIIFLTTIMFIMSTVMVFAEEDIDTIALANAEKFGILT
                     LIPPLVAIILAFITKNVIISLLIGILSGSFIIKASGINVFATFIQAFLDLVDRALVSL
                     ADPWNAGIILQVLAIGGVINLVAKMGGAKAIAEALAKRAKSAKGTQLITWFLGLLVFF
                     DDYANSLIVGPMMRPVADKMKISREKLAFIIDATAAPVAGLAIISTWIGLEVGLIHDA
                     FESISIDVDAFGIFLNTIPFRFYNILILAFIVISALLLKEFGPMRKAEIKSRSRKISI
                     DLDEGVEELDDLAPKNGVKLSVWNAIIPIGTLIIVALASFYYSGYTSIMGGDDKALIQ
                     LFTNSPYSFEAIKEAFSASDASRALFQSALVASLVAIIMAVVKKIFTISEAIDVWIDG
                     MKSLVITGVILILAWSLSSVIKELGTAKFLIHLLSGSLPPFLLPSLIFGLGAIISFAT
                     GTAYGTMGILMPLAIPLAYSLNPDMSYVIVSTSAVLTGAIFGDHCSPISDTTILSSMG
                     AGCNHIDHVNTQMPYAIFTAVITIVFGYIPAGLGLPIYIVLPVAIAAIFVGIQIIGKK
                     VDEAEIELVE"
     gene            7494..7574
                     /locus_tag="KPD58_001718"
     tRNA            7494..7574
                     /locus_tag="KPD58_001718"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7528..7530,aa:Leu,seq:tag)
     gene            7909..8424
                     /locus_tag="KPD58_001719"
     CDS             7909..8424
                     /locus_tag="KPD58_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HBG0265490.1"
                     /translation="MEKYIWLFPLLFIFHDMEEIIGFGIWLKKNKSMLDKKYPFISKV
                     YENYSTEGMAFAVFEEFILCIIFCILTVITENQYVYLLWLGSFIAYTLHLVIHIGQSI
                     IIRKYIPSLITSIICLPISIWCISKSIYIVDCEMSTTILYSIIGIIIVALNLKFAQSL
                     IGKFTKWMSNI"
     regulatory      8916..9020
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            9230..11704
                     /gene="gcvPA"
                     /locus_tag="KPD58_001720"
     CDS             9230..11704
                     /gene="gcvPA"
                     /locus_tag="KPD58_001720"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HBG0265491.1"
                     /translation="MNELKRVSLYNIHKELGAKLVEFAGWEMPLEYEGINKEHEKVRK
                     SAGIFDVSHMGEVQIKGAESEKFIQNLVTNDISTLKINDIIYTPMCYENGGVVDDLLI
                     YKFGEEDYLLVINAGNIDKDVAWIIKQSEGYNVDIKNISSEVSQLAIQGPKAEEILQK
                     ITDIDLNSIKFYKSIPSTKVCGCPCLVSRTGYTGEDGFEIYCKNKYVEIIWNEVLKVG
                     GEDICPAGLGCRDTLRFEAALPLYGHEINEHISPIEGGLSIFVKTNKESFIGKSILSK
                     EKESGAKRKLVGFEMQGKGMPRNGYDIRIGDKTVGFVTTGCASPTTGKILGMGIIDSE
                     YAKVGNEIGIAIRKKVVPAVIVKKPFYKKQYKKDNIILNKENKFSYIPATSEDKSKML
                     KVVGLNSVDELFSDIPEEVKLKRDLNLEIGKSELEVSKIVKRLSEENLSLEDLTCFLG
                     AGAYDHYIPSIIKHITSRSEFYTAYTPYQAEISQGTLQVVFEFQSMIAEITGMEIANA
                     SMYDGATAAIEACIMAMNQTRKSKIVVSKTIHPETLSVLRTYLQYKDCEIVEIDFCNE
                     YGTTDIEKLKASVDKDTACVLIQTPNFFGIIEEMEEIEKITHENKAMLIMSVDPISLG
                     VLKTPGEIGADIVVGEAQSLGNPLNFGGPYVGFLASKSKYTRKMPGRIVGQSLDVEGK
                     IAYVLTLQTREQHVRREKATSNICSNQALNALVASIYMATMGKEGFKEVGMQSMKKAH
                     YTYNKLVQTGKYKPIFKGKFFKEFAVQGNLNIETINDKLLEENILGGYNLEYNYPELK
                     NSTLLCVTEKRSKEEIDKLVGIMEGL"
     gene            11704..13161
                     /gene="gcvPB"
                     /locus_tag="KPD58_001721"
     CDS             11704..13161
                     /gene="gcvPB"
                     /locus_tag="KPD58_001721"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HBG0265492.1"
                     /translation="MKEYNSLLIDISKKGRKAYSLPKLDIDDIKIEDMIDPDMARQSE
                     LNLPEVGELELVRHYTLLSNKNFGVDTGFYPLGSCTMKYNPKINEDMAALPNFTGMHP
                     YQSSDTAQGSLSLMYDLSRRLAEITGMDEVTLQPSAGSHGEFTGLMIIKAYHENRGDH
                     KRTKVIIPDSAHGTNPASAAMANFDVIQIASDKNGAVDINVLKEVLNDEVAALMLTNP
                     STLGLFEKNIKEIATLVHEAGGLLYYDGANMNAIMGITRPGDMGFDVVHLNLHKTFST
                     PHGGGGPGAGPVGVKKELVPFLPIPVIERKEDKYVLNYDREKSIGKIKNFYGNFGVLV
                     RAYTYILTMGRDGLKEASEMAVLNANYIKESIKDDYILPIDTLCKHEFVLGGLPRGEA
                     NIKTIDIAKRLLDYGYHPPTMYFPLIINEALMIEPTESESIETLDSFIEAMKSVAKEA
                     KEEPELLKTAPHNTLVKRVDDARAVKKPILTWSQR"
     gene            13584..15941
                     /locus_tag="KPD58_001722"
     CDS             13584..15941
                     /locus_tag="KPD58_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HBG0265493.1"
                     /translation="MDKVNLERYSCDINVGLNKNQLQSRIDDNLINAFDNGKTKTYKQ
                     IFKDNIFTLFNLINIVLVCLLVSVGSFKNTLFIFIAVINTIIGVIQEIRAKRLLDNLS
                     VIVSNKVSVIREKEKKEIDVQELVLDDIMILKTGNQICADSKVLNGKLEVNESLVTGE
                     SDIIIKNKGDFLYSGSFVVSGEAFVRVENIGKDNYANKIANDAKISKKHNSQLRNSLN
                     AILKIVGIIIIPLGIILFAKQHFGNGDSIATSVVGTVAAVNGMIPEGLTLLTSIALAV
                     GTIKLASHKTLVQELYCVETLARVDTLCLDKTGTITEGKMQVDDIVMLEEVDINEIMG
                     NICNSLKDDNATLNAIRDKYNVLDTYKAKNLIPFSSERKYSGVTFENKGTYLLGAFEF
                     IFKEKNKKLRIEVERYSKKGNRVIVLAHSDSYMEDDNIPEDIKPLAFILILDKIRDEA
                     KETLEFFYNEGVDIKIISGDNPITVSEVARKAGLKTASNFIDATTLKNDSDIYKAVDK
                     YSVFGRVSPQQKKQMILALKKQNHTVAMTGDGVNDVLALKEADCSIAMASGSDVTKNV
                     SNLVLLDSNFASMPKVVMEGRKVINNIQRASSLFLVKTIFSFFLSLLTLFFFSRQYPF
                     VPIQLSLIGAVTVGIPSFFLALEANKSRISGNFLLNILKNALPGALCVTVNVIIVYNI
                     VEYLGYSSSVFSTMCAILTGVCGLLILRQQCLPFNKERLFLIVSMTIAFVIGILFLGG
                     LFSFVALSRELILITMALAILMPIMIKVMLFVLDEILETIVPDLN"
     gene            complement(16027..16344)
                     /locus_tag="KPD58_001723"
     CDS             complement(16027..16344)
                     /locus_tag="KPD58_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HBG0265494.1"
                     /translation="MSEFKNVTAVKKANVYFDGKVSSRVIILPNGERKTLGLMLPGEY
                     TFSTREEEIMEMLAGSMDVKLPGSNEFVTYKEGQKFNVPSDSSFDLKVNEVVDYCCSY
                     IAD"
BASE COUNT         6233 a         1923 c         2697 g         5662 t
ORIGIN      
        1 aaagtttata aattatatta atagtaataa tctataagaa cttatatatt agaaaaaaat
       61 ttttgtatat ataaaggact ctaaagatga actatgtaaa acaaggttta tatattctaa
      121 ttgtatagaa aaatttattg taaacgcatg tatattatta gaaaaaatag tttggaggta
      181 ttcaataaac ataaaaaaca atatagggga taaaggtgtt ttatgaagag atttttgagg
      241 agaattatct tagttttatt tattttgttg atttttataa gttttatttc tattaaatta
      301 attcataatg ttggagatta tggaaaattg attaattatg ttgggattgt tcgtggagct
      361 agccaacgtc taactaaact agagatgaat catcaaccaa atgatgaatt aatagaatat
      421 attgatgaaa tcctacagga gttgattaca gggcatggag attatggttt agtaatcact
      481 gattgtaatg aatacaatga ggacttactt ttattagaaa aaaagtggga agatttaaat
      541 attgaaatta aaaaagtaag aatgaaagaa cagaataacc agttattaag tataagtgaa
      601 gagttttttt ctcttgcaaa tgatacagtg tttaaaatag aaaacttttc taaagaaaaa
      661 agtaattact taatgacttt aataataatt atatctatta taggtatatt agcatgtatc
      721 atattgattt tacaatatag taaaaaaatg gttagactgg aaaaattaaa tgtagattta
      781 aaaaatattg catataaaga tgaactaaca ggagtaaata ctatagaaaa atttaaattg
      841 gatgcaaatc aaaatatttg tatacataag gataagaaat ttgctgtatt ctatattgat
      901 tttgaaaact ttaaatacat taatgatata tttggatatg attatggtga catgatactg
      961 aaaagatatg ctaatcttat gatgaatgac atagggaaat atgaaatatt tgcaagagaa
     1021 attgcagata gatttgttgc gttaagatgc tatgtagata aggaagattt agtggtaaga
     1081 caacgagatg tagacaatga actaattaat ataacaaatg aaattaaaaa taaacatagt
     1141 attactgttg ttagtggaat ttgttgtatt gaagatgtaa atgaaaaatt aagtatagat
     1201 ggtttaataa atagagcaaa ttttgcacaa aaaactgtta agaataaacc aggtactaat
     1261 tatgcttttt ataatgacag tattcgtaaa aaaatgattg aagaaaatac tatcaaaagt
     1321 agaatacatg aagctattga aaaaagagaa tttatagttt atttacagcc taaagttaat
     1381 ttacatagtc aaaaaataaa ttgtgcagaa gctttagttc gttggcttac tccggataaa
     1441 ggtattatat ctccagcaat atttattcca gtacttgaga agaatttttt tattgcctta
     1501 gtagataagt atgtttttga ggaggtttgt aaatggatta gaaagaggtt agatgaaaat
     1561 aaaccttttg ttcagatatc agttaatgtt tcaagaatac aattttataa cactaaattt
     1621 gtagaaactt attccaatat acaaaataaa tacaggatac ctaaaaatac tatagaaatt
     1681 gaatttacag agagtgttgc ttttgagaat caaaaacatc ttttggaaat tatacatgat
     1741 ttgcatgaaa atggatttac ttgttcttta gatgattttg gaaaaggtta ttcgtcttta
     1801 agtgttttaa aagatttacc atttgatgtt ttaaagcttg attctatgtt tttcaaggct
     1861 agtttagata aagataaaga gaaaatagta ataaaaaata ttgtacatat gttaaaagaa
     1921 cttaatatca caactgttgc tgaaggcatt gaatgcaagg aacaagttga atttcttagg
     1981 gatattgggt gtgatttagt tcagggattt gtattttata aaccaatgcc aatattagaa
     2041 tttgaaaaaa tattggataa agagtttgtt tataatagct aaaatgtact ttataaaata
     2101 tgaactaatt aagtaaaatt attttgtact gaataaattt ttactttatt tacagaataa
     2161 tgattattta cagaaaagcg cgaaaagtgt attcctttaa gtgtgggatg gatagcacaa
     2221 ataggggaga aaaaatattg gccatataag aatgcataat aatataaata tttgaaaggt
     2281 cagcattata taaaaattaa aaagaaaata aataattttt ctataaaaaa gagactatga
     2341 gaaaatttac tcatagtctc ctaaataatg tatttatttt cttcctgcaa cccattttaa
     2401 tccccaatga aattcagtat ccatttctct atgacctttg aagtatttaa ctactttttc
     2461 aacactgaat gtctcatcat ttacattttc tatcaatgct attgaacaca tattatagcc
     2521 atttttatgt tcatcaagtt tgacaactaa gtctgaatct tgtttttgtt tgattgttac
     2581 aattccatca gcttcagacc agttagctac accttcgtat ataaaagcat aaataagtat
     2641 tctttttata tcttttattt tattaccatt tattcttaaa ttttctcctt gtgtattact
     2701 tcctgttctg tcatctccat ctaattgagc aaaaggtgga ttgtctaaac taccaaaggc
     2761 atttcctaat gcttgaacag caccttttac accatttttc atctcaaata ggcaacccaa
     2821 atctaaatct atattactgc ttcttaaaga gccaaaaaaa cccttttttt gtactttttg
     2881 attccaattt aaattaacta agatttcacc taaatcgcta ttttcctttt ttgtaaggtt
     2941 tattttttgt ccctttttta attctatagc catagtaatc ttcctccaca aacatagatt
     3001 tatttaaatg gtagcactta ataagtcaat attcaacaag attagaataa taatattaaa
     3061 attatattaa atatactaaa aagtaggaat cacaatacct ttgtattttt cttcaattaa
     3121 gcttcttgct ttatctgatg taagaacatc ttgtagaact ttaatctttt cactgtcttt
     3181 atcttcagtt ctaaatgcta aaagatttgc atactcgtca tttataggag ctgtataaat
     3241 tccgtttttc ttggcatcaa gaccagcagg tacagcataa gtagtatcta taaatgcagc
     3301 agtcacatct ggaagagaag atggaatttg agcaatatct aactctttga attgtattcc
     3361 ctttggattt tctattatgt ccttaggact aactaatcca tcccctttag gtaacgttaa
     3421 aagtccaata ctttcaaggt atctaaggtt cttagaacaa gaagatgggt tattgcttat
     3481 tgcaattgta tctccttttt taaattcatc aacagatttt atcttataag agtaaagtat
     3541 tgctggacat acatatagtt ttttaccagc cattattttg taacctttgg ccttaacagc
     3601 ttcttttaga taaggttcat gttgaaatag atttgcatca atttcaccat tatttaaagc
     3661 ttcatttgga agtatgtaat catcaaagtt tttaacttct aaagtatatc ctttttcttt
     3721 aataagtggt tttaattctt ctaataactc tcctcctgga actaaagtag caccaacaac
     3781 aattttttta tcatctttag ctttagaaca tccaaccaca gctaaagtta atactagaca
     3841 taataacaaa cataataatt ttttgaattt catataaatt ccctcctgaa tataattgat
     3901 tagttaaaaa taaaatacca ttctttatca cactgcaaaa taaaaataag cctgagttta
     3961 aactcaggct ttaaaagcta atttagactc atctttcagt ataatactgc aggatttagc
     4021 accaatgtat atagaaaata tactggttgc tgggtttcat agggccagtc ccttcaccac
     4081 tctggataag aagtatcaat ttttaattat gttataaata ttatcatgat tttttacgtc
     4141 tgtaaacata attttataaa tattttaaaa tctttaatgt attttattta acacctaaaa
     4201 ttccagaaac tagaatatca gtatcagcac ctaagaaata gtcatttatt tcaatagaat
     4261 ccagagtatt tctcaaagca ttttcttcat gccttatacc aataagttta ttttctacaa
     4321 atgcaacatc acattttcca aaaaaatctc caaaaaattt tatactagtt attatgcctt
     4381 tatcaacatt taaattaaac tcaacatttc caccagaata ttttaattca tttgaaagag
     4441 tatattttgg tgaattacca aaattccaat cccaagtttc gtattttgat ttagtaagct
     4501 tttctatttg ttttatatca tgctcactca aacaataatt agtattatca gtatttgttt
     4561 tagaaagata atccattata gcatctttaa attctaatat atccattttt gaatgtaaat
     4621 gttcacttat attggttaca cgagatttta cagattttat tccttttcct tcaaatttta
     4681 taggtttaac ctttaatgct tttgagaggt catttatttc agatgaaaat aaaagagtcc
     4741 catgatgcat tactttgttt ttgtagttgt attgagcatt tccagaaaat ttcttgccat
     4801 ctataagaag gtcatttctt ccagaaaatt cggcattgac atctagtgtt ttaagtaaat
     4861 ctattatggg ctgagtgaat cttctgaaat cactaaaatt attattgtta caagctataa
     4921 atgtaaaatt tatatttccc aaatcatgaa atacagctcc accaccagac tgtctcctaa
     4981 cgacaggtat agaatattct tttatatatt ctaaatttat ttcagaaagt gtgttttggt
     5041 tttttccaac aattatagaa ggttcattcc tccataaaat aaataagtct tcatcaaaat
     5101 tttttagaag atattcttcc attgctaaat taaaataagg gtttgttttt tcattataaa
     5161 ttaataacat taaagtagtt cctccataga attgataaaa tttttattat gaatacaatt
     5221 aattttataa ttcgacaata atataaaaac tcctttaaat tagaaatttt aaaaactacc
     5281 ttgcaaagtt taaataaata atatatactc aaaatatgtt taataaaagt gaatagaagg
     5341 gtagaggcgc aataatctat cagtactaat aaggagttaa gcacagagat ttattaggaa
     5401 aggagatatt gccgaagttt tataatcgat gctttaagat tatattgctg ggggtacata
     5461 gaaaatatgt acaactgtca caggtttatt tgtggtgagc tatcatttaa ggaattatat
     5521 tatatttatt tttactattg ttgatatatt gtaatgttta agccttgagt gtttaccact
     5581 caaggctttt ttagttttat tgtaacataa taccctgtac agttcacaaa attaaaagga
     5641 gagggagatt tatatgagga acaaaaaaat taatattatt tttctgacga caatcatgtt
     5701 cataatgtca acagtaatgg tttttgcaga agaagatata gacactattg cactagccaa
     5761 tgcagaaaaa tttgggattt taactttaat accaccatta gttgctataa tacttgcatt
     5821 tataactaaa aacgtaataa tttctttact tataggtatt ctttctggta gttttataat
     5881 taaagctagt ggtattaatg tttttgcgac atttatacag gcatttttag atttagtaga
     5941 cagagcttta gtatctttag cagacccttg gaatgcaggg ataatactac aggttttggc
     6001 aattggagga gtaataaatc ttgttgctaa aatgggagga gctaaagcta tagctgaagc
     6061 acttgctaaa agagcaaagt cagcaaaagg gacacagctt ataacatggt ttttggggct
     6121 tttagtattc tttgatgatt atgccaattc actaatagta ggtcctatga tgagacctgt
     6181 tgcagataaa atgaaaatat cgagagaaaa gctggcattt ataatagatg ccacagcagc
     6241 accagttgct ggtttagcta taatatctac atggataggt cttgaggttg gtttaataca
     6301 tgatgctttt gaaagtataa gtatagatgt agatgcattt ggtatatttt taaatacaat
     6361 accatttaga ttttacaata tattaatatt agctttcatt gtaatatcag cattattatt
     6421 aaaagaattt ggtccaatga gaaaagctga gataaaatct agaagtagaa aaattagtat
     6481 agaccttgat gaaggcgtag aagagttgga tgatttagct ccaaaaaatg gagtgaaatt
     6541 gagtgtatgg aatgctataa tcccaatagg aacattgata atagttgcat tagcatcatt
     6601 ttattatagt ggatatacat ctataatggg aggagacgat aaggctctga tacaactatt
     6661 tacaaattct ccatattctt ttgaagcaat aaaagaagca tttagtgcat ctgatgcatc
     6721 tagagcatta ttccagtctg cacttgttgc aagtttagtt gctattataa tggctgtagt
     6781 aaaaaagatt tttacaatat cagaagcaat tgatgtttgg atagatggaa tgaaaagttt
     6841 agtaatcact ggtgtaatat taatacttgc atggtcatta agttcagtaa taaaagagtt
     6901 aggtacagct aaatttttaa tacacctatt atcaggttca ctaccaccat tcttattacc
     6961 aagtttaata tttggtttag gagcaataat atcttttgct actggaacag cttatggaac
     7021 aatgggtata cttatgccac ttgcaattcc attagcatac tcattaaatc ctgatatgtc
     7081 ttatgtaata gtaagtacta gtgcagtttt aacaggtgca atatttggag accattgttc
     7141 tccaatttca gatacaacaa tactttcttc aatgggagct ggctgtaatc atatagacca
     7201 tgttaacact caaatgccat atgctatatt tacagcagta atcactattg tatttgggta
     7261 tataccagct ggacttggat tgccaatata tatagtatta ccagtggcaa tagctgctat
     7321 atttgtaggt attcaaataa ttggtaagaa agttgatgaa gcggaaatag aacttgtaga
     7381 ataaagatat aaataagttt caccttttat tatcactatt ttgttagttt ttagataatt
     7441 ttaaaaagtt attgaaaata atggcgaaat atagtaaaat aaaagagtga taagcggatg
     7501 tggcggaatt ggcagacgca ctagacttag gatctagcgc cattggcgtg ggggttcgac
     7561 tcccttcatc cgcactttac agtacttaat tgtactgaaa aaaactgggg ttgctggctt
     7621 tcggagtggg ctatcccgtt tttatttttt tgtatatata atagattggc actttagtat
     7681 aaggtgcttt ttttataggc aaaaacagta catttatttc tgttttatta tcattcatct
     7741 attagaatat actttagtta agaatgtgtt aataagcagt gttcacattc ttgataaaat
     7801 gtggaataat atgtattttt actaagacgg aaatgaaata attaaaccat taaataatat
     7861 tataagatga tgaataagta gataaatcaa attttgtagg aggatattat ggaaaaatat
     7921 atatggttgt ttccattact ttttattttt catgatatgg aagagataat aggttttgga
     7981 atatggctta aaaaaaataa atcaatgctt gataagaagt atccatttat cagtaaagtt
     8041 tatgaaaatt atagtactga aggcatggca tttgctgtgt ttgaagaatt tattttatgc
     8101 attattttct gtatacttac agttataaca gagaatcagt atgtatatct actatggtta
     8161 ggtagtttta tagcatatac cttacatttg gtaattcata tagggcagtc aatcataata
     8221 agaaaatata taccttcact tattaccagt atcatatgct taccaataag catttggtgt
     8281 ataagtaaga gtatttatat agttgattgt gaaatgagta caacaattct gtacagtata
     8341 attggcatta taattgtagc attgaattta aagtttgcac agtcgctgat tggaaaattt
     8401 acaaagtgga tgtcaaacat ataaatttta gtttattgga cagttcaagg aatcagaaat
     8461 tggaatttta tagtattaat attttatagt attaatataa tgtataaaat tttatagcaa
     8521 tgtatttcat aagtataaag tgactagaat tttaagaaat atacaaattc tataaaaact
     8581 aaataatttc aagtatattt taaaaatctt atttttcaca agtttaaaat tgtaaacgat
     8641 ttcatcaagt ctatgaatat acttttaaca tgttatagta aatctcaata taaatttaca
     8701 tttggttctt tgtgtgttat tttaataata aattaagtga aaatgttatt ttacattaat
     8761 atataatatc tttgcataag agtgaataat ataaaagaga aataattgat aataaaaact
     8821 taacaaaaaa attaatacat aaaaaaaaat aaatataatg tgttgacatt taattgaaat
     8881 attcatacaa taaaattgaa aataaatatt tccaaatgag attagcgaga gagtccttta
     8941 tacgaaaagg cgccgaagaa gaaatttaag aaatgccaat ttttagagaa gctttcaggc
     9001 aaaaatatcg ctaattgatg ggactctgga gagacttaag ttaagatact ataagtcacc
     9061 gaagaagcaa aataatatta aattattaaa tctttcaggt atacaaacag ggatatatag
     9121 gctaggatta ttttccatgc tgatatatct tttttttatg aaaaagtaca ggaaataata
     9181 tcaaagttac ttaaatacag taggtctaat tttctaggga gggttatata tgaatgaatt
     9241 gaaaagagta tctttgtaca atatacacaa agagcttgga gcaaaacttg ttgaatttgc
     9301 tggttgggaa atgcctttag aatatgaggg tataaataaa gagcatgaga aggtcagaaa
     9361 aagtgcaggt atatttgatg tatctcatat gggagaggta cagataaagg gagcagagtc
     9421 agaaaaattt atacaaaacc tagttacaaa tgatataagc acattaaaga taaatgacat
     9481 tatatataca cctatgtgtt atgaaaatgg tggagtagtt gatgatttat taatatataa
     9541 atttggtgaa gaagattatt tattagtaat aaatgcaggt aatatagata aagatgtagc
     9601 atggataata aaacaaagtg aaggatacaa tgtagatata aaaaatatat ctagtgaagt
     9661 gagtcaatta gcaattcaag gacctaaagc agaagaaatt cttcaaaaaa tcacagatat
     9721 agacttaaac tcaataaaat tttataagtc aattccatca acaaaagtat gtggttgtcc
     9781 ttgtttagtt tcaagaacag gatatactgg ggaagatggg tttgagatat actgtaaaaa
     9841 taaatatgta gaaataattt ggaatgaagt tctaaaggtt ggaggagaag atatatgtcc
     9901 agctggtcta ggttgtaggg atactttgag atttgaagca gctctacctc tatatggaca
     9961 tgaaataaat gaacacatat ctccaattga aggtggttta tctatctttg tgaaaacaaa
    10021 taaagaatca tttattggca aatcaatttt atcaaaagaa aaagaaagtg gagctaaaag
    10081 aaagttagta ggatttgaaa tgcaaggtaa aggaatgcct agaaatggat atgatataag
    10141 gattggtgat aaaacagttg gatttgtaac aacaggatgt gcatctccaa cgacaggaaa
    10201 aattttaggt atgggaatta tagattctga atatgcaaaa gtaggaaatg aaataggtat
    10261 agcaatcaga aaaaaagttg ttcctgctgt gatagttaag aaaccttttt ataaaaaaca
    10321 atataaaaaa gataatatta tattaaacaa agaaaataaa ttttcatata tacctgctac
    10381 aagtgaagat aaatcaaaaa tgttaaaagt agttggatta aattcagttg atgaattatt
    10441 ttcagatata ccagaagaag taaaattaaa aagagattta aatttggaaa ttggaaaatc
    10501 tgaattagag gtaagcaaaa tagtaaaaag attgtcagaa gaaaatttaa gtttagaaga
    10561 cttgacttgt tttcttggtg ctggtgcata tgaccattat ataccatcaa ttataaaaca
    10621 tataacttca agatctgaat tttatacagc atatactcca tatcaagctg aaataagtca
    10681 gggaacttta caagtagttt ttgaatttca atctatgatt gcagaaatta caggtatgga
    10741 aattgcaaat gcatcaatgt atgatggagc aactgctgca atagaagcat gtataatggc
    10801 aatgaatcag actagaaaaa gtaaaatagt agtgtcaaaa actattcatc ctgaaactct
    10861 gtcagtatta agaacttact tacaatataa agattgtgag atagtagaaa ttgacttttg
    10921 taatgaatat gggactacag atatagaaaa attaaaagct tctgtagaca aagatacagc
    10981 atgtgttctt atacaaactc ctaatttctt tggaattatt gaagaaatgg aagaaataga
    11041 aaagataact catgaaaata aggctatgtt aattatgagt gtagacccaa tatcattagg
    11101 agttttaaaa acaccaggtg agataggtgc agatattgtt gttggagaag ctcaatcact
    11161 tggaaaccct cttaattttg gaggtcctta tgtaggattt ttagctagca aatctaaata
    11221 tactagaaaa atgccaggaa gaatagttgg gcaaagttta gatgtagaag gtaaaattgc
    11281 atatgtatta actttacaaa ctagagaaca gcatgtaaga agagaaaaag caacttctaa
    11341 tatatgttca aatcaagctt taaatgctct tgtagcttca atatatatgg ctactatggg
    11401 taaagaagga tttaaagagg ttggtatgca gtctatgaaa aaagcacatt atacctacaa
    11461 taaacttgta caaactggaa aatataagcc aatatttaaa ggtaagtttt tcaaagaatt
    11521 tgctgtacaa ggaaatctta atatagaaac tataaatgat aaactcttag aagaaaatat
    11581 attaggtggt tataatttag aatacaatta tccagaatta aaaaattcaa ctttgctttg
    11641 tgtgactgag aaaagaagca aagaggaaat agataagtta gttggaataa tggagggatt
    11701 ataatgaaag aatacaatag tttgttgata gatatatcta aaaaaggtag gaaagcatac
    11761 tcacttccaa agttagatat agatgatata aaaatagaag atatgattga cccagatatg
    11821 gcaagacaaa gtgagttgaa tttgccagaa gttggtgaat tagaattagt tagacattat
    11881 acattattat caaataaaaa ctttggagtt gatacaggtt tttatccttt gggttcttgt
    11941 actatgaaat ataatcctaa aataaatgaa gatatggcag cacttccaaa ctttactgga
    12001 atgcatccat accaatcttc ggatacagct caaggttctc tttctttgat gtatgattta
    12061 tcaagaagac ttgcagaaat tacaggtatg gatgaagtta cactacaacc ttcagcaggt
    12121 tctcatggtg agtttacagg gcttatgatt ataaaagcat atcatgaaaa tagaggtgac
    12181 cataagagaa ccaaagtgat aattccagat tctgcacatg gaacaaatcc agcaagtgca
    12241 gctatggcaa actttgatgt gattcaaata gcatctgata aaaatggagc tgtagacata
    12301 aatgtattaa aagaggtttt gaatgatgaa gtagcagctc ttatgcttac taatccaagt
    12361 actcttggtt tatttgaaaa aaatataaaa gaaatagcta ctcttgtaca tgaagcagga
    12421 ggacttttgt actatgatgg tgcaaacatg aacgctatta tggggataac aagacctgga
    12481 gatatggggt ttgatgtggt tcatcttaat cttcataaga ctttttcaac tcctcatgga
    12541 ggtggaggtc caggagctgg tcctgttgga gttaaaaaag aattagtacc ttttttacca
    12601 ataccagtaa ttgaaagaaa agaagataag tatgtactaa attatgatag agaaaaatct
    12661 attgggaaaa ttaaaaattt ctatggtaat tttggagttc ttgtaagagc ttacacatat
    12721 atactaacta tgggaaggga tggacttaaa gaggctagtg aaatggcagt tttaaatgct
    12781 aattatataa aggaaagtat aaaagatgat tatattttgc caatagatac cttgtgtaag
    12841 catgagtttg tattaggtgg attgccaaga ggagaggcca atataaaaac tatcgatatt
    12901 gcaaaaagac tactagatta tggataccat cctccaacaa tgtactttcc tcttattata
    12961 aatgaggcac ttatgataga acctacagaa agtgagagta tagagacatt agatagtttt
    13021 atagaagcta tgaaaagtgt tgctaaagag gcaaaggaag aaccagaact attgaaaact
    13081 gctccacata atactttggt caaaagagta gatgatgcaa gggcagtaaa gaaacctata
    13141 ctaacatggt cacaaagata aatagttaaa aagtagagat ttaaagtata aagagaatta
    13201 aatttataag aaaaagctat ctaacttaat ctagtttaga tagctttttt gtatattagt
    13261 atatatggta tttcctatat aaagttttaa attatattag tgcttatata gaatattgat
    13321 atgatgatta tgtatagata ttaataagaa ttgttgaatt tatcttgcct taactttatt
    13381 tactctttta tattaagaaa aacttaacta aaatatagcg aaattcataa aaatttagga
    13441 aatcttaaga attataatat ttaaatcttt agatttatat tttattatat aaactataga
    13501 ataataaaat aaaaaaattg agaattttat aatatatttg tagatttgag tataaaaaag
    13561 tgctaagttg aaaggagtaa aatatggata aggttaattt agaaaggtat agttgtgata
    13621 taaatgttgg tttaaataaa aatcaacttc aaagtagaat agatgataat ttaattaatg
    13681 catttgataa tggaaaaacc aaaacatata aacaaatttt taaagacaat atatttacac
    13741 tttttaatct cataaatatt gttttagtct gtttattagt ttcagttggt tcttttaaaa
    13801 ataccttatt tatatttatt gctgtaatta atactattat aggagtaata caagagataa
    13861 gagctaaaag attattggac aatctatctg ttatagtttc aaataaggta tctgtcataa
    13921 gagaaaaaga gaaaaaagag attgatgtac aagaattagt attagatgat attatgattt
    13981 taaaaacagg taatcaaatt tgtgcagatt caaaagtttt aaatggaaaa ttagaggtaa
    14041 atgagtcctt ggttacaggt gaatctgata ttattataaa aaataaaggc gattttttat
    14101 attctggaag ttttgtagtt tctggagaag cttttgtaag agttgaaaat ataggaaaag
    14161 ataactatgc aaataagata gctaatgatg caaagatttc taaaaaacat aattctcaac
    14221 ttagaaattc attaaatgca atattaaaaa ttgtaggtat aataataatt cctctaggaa
    14281 ttatattgtt tgcaaaacaa cattttggaa atggagattc tatagctaca tctgtagtag
    14341 gtacagtggc agctgtaaat ggtatgatac cagagggatt aacattactt acgagcattg
    14401 ctctagctgt aggaactata aaacttgcta gtcataaaac tttagtgcaa gaactgtact
    14461 gtgtagagac tcttgctaga gttgatacac tatgtcttga taagacaggt acaattacag
    14521 aaggtaaaat gcaagttgat gatatagtaa tgttggaaga agtggatatt aatgaaataa
    14581 tggggaatat atgtaattct ctcaaagatg ataatgcaac actaaatgca ataagagata
    14641 agtataatgt tttagatacg tataaagcta agaatttaat accattttct tcagaaagaa
    14701 aatacagtgg ggttaccttt gaaaataaag gtacatattt attaggtgca tttgagttta
    14761 tatttaagga aaaaaataaa aaacttagga tagaagttga aagatattct aaaaaaggaa
    14821 acagagtaat agttttagct catagtgatt cttatatgga agatgataat attcctgaag
    14881 atataaagcc actagctttt atattgatat tggataaaat tagagatgaa gctaaggaaa
    14941 ctttagaatt tttttacaat gaaggtgtag atataaaaat tatatctggt gacaatccta
    15001 taacagtaag tgaggtagct agaaaagcag gtcttaaaac agctagtaat tttattgatg
    15061 caacaacact taaaaatgat agtgatatat acaaagcagt tgataaatac agtgtttttg
    15121 gaagggtaag cccacaacag aaaaaacaaa tgattttagc attaaaaaaa caaaatcata
    15181 cagtagctat gactggagat ggtgtaaatg atgtgctagc acttaaagaa gcggattgta
    15241 gcattgccat ggcatctgga agtgatgtta ctaagaatgt gtctaattta gtcttattag
    15301 attcaaactt tgcatcaatg ccaaaggttg ttatggaggg aagaaaagta attaataaca
    15361 ttcaaagagc atcttctttg tttttagtaa agacaatttt ttcattcttc ttgtcgttat
    15421 taacattatt cttcttcagt agacagtatc catttgttcc tatacaatta tcactcattg
    15481 gggctgtaac agtgggtata ccatcattct tcttagctct tgaagcaaac aaaagtagaa
    15541 tttcaggaaa ttttttactc aatatattaa aaaatgcttt accaggagca ctatgtgtga
    15601 cagtaaatgt aattattgtt tacaatatag ttgaatattt gggttatagt tcaagtgtat
    15661 tttccacaat gtgtgctata ttaactggtg tatgtggtct tttaatatta agacaacaat
    15721 gtttgccatt taataaagaa agattatttt taatagtaag tatgactatt gcatttgtta
    15781 ttgggattct atttttaggt ggattattct catttgtagc attgagtaga gaattaattc
    15841 tcataactat ggcattagca atattaatgc caattatgat aaaagttatg ttatttgtct
    15901 tagatgagat attagagaca attgttccag atttaaatta attttaaaat gatataaata
    15961 agagcagttt tagttgaact gctcttttta gatgtgttta attttatgta agaatatgta
    16021 tttttattaa tcagctatat atgagcaaca gtagtcaaca acttcattaa cctttaagtc
    16081 aaaacttgaa tcactaggaa cattaaattt ttgtccttct ttgtaagtta caaactcatt
    16141 gcttccaggc agttttacat ccatacttcc agcaagcatc tccattattt cttcttctct
    16201 agtagaaaaa gtgtattctc ctggcaacat taatcctaaa gtttttcttt ctccattagg
    16261 cagtattatt actctgctac ttaccttacc atcaaaataa acgtttgcct tctttactgc
    16321 agtaacattc ttaaattcac tcattagata aaacctccaa aattatttgt acatatatat
    16381 tatataaaaa taatgtaaaa aatgctatat atttacaaaa aaatttagta tttattaaaa
    16441 aaattaataa gaataaaaaa gagatggaat tgcttaaatt gataaaaaat ataaaataaa
    16501 ttgcaaagta agcaa
//
LOCUS       DAELLV010000066        16473 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.066, whole genome shotgun sequence.
ACCESSION   DAELLV010000066 DAELLV010000000
VERSION     DAELLV010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16473
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.066"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            51..713
                     /locus_tag="KPD58_001724"
     CDS             51..713
                     /locus_tag="KPD58_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HBG0265495.1"
                     /translation="MIDISIDDKLKERWPNAKLGCIQAKVAVNKSSEKLINEINEFCD
                     TINQSLKVEDITKLDKIKDARNAYKELGKNPSRYRTSSEALVRRIIQGKGLYTINNIV
                     EINNLISIKSLYPVGTYNVSKLHSPVCFTVGDEGEQYKGIGKELINIENLPILSDSVG
                     KFGSPTSDSERAMITEDANEILICIFSFSQESDIEEYFGYAKELLKEYADGKDIETKI
                     IK"
     gene            1084..2493
                     /locus_tag="KPD58_001725"
     CDS             1084..2493
                     /locus_tag="KPD58_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HBG0265496.1"
                     /translation="MIDGKEVKQESGQKNLKFKIAIVVGVLFVAYIGMAIFFRNHFYF
                     GTTINGVKASGKTVEQVNDLLTSSTNSYTLNLEERNNKKEQIKAKDIDLKFTPDDKVQ
                     KIKDSQSAIGWIGGIFKSKEYNNMITLSYNKDLLEKRFNALSCFDSKNVVEPKSAYPK
                     YDSTKNAYVIVPEVLGNKVKKDDFYKLVQNSINSGNTSISLDESKVYEAPNNTKDSEK
                     LKQAIKTLDKYVGVVVTYDFSDRKETLDGSEINKWLKVDSEKNYDITFDNDAMVEYTR
                     GLSKKYSTFGDSRPFKTATGKDITISGGIYGWLIDKKKEAEALVDVVKAGKNVTREPI
                     YIQKAVSRNKDDIGNTYVEISLSGQHMWFFKNGEMLVSTDVVTGDLARGFATPSGIYP
                     LNYKARNVSLTGQGYSSPVSYWLPFNGNIGIHDATWRNSFGGSIYKSSGSHGCVNTPF
                     SKAKKIYENIEPGTPIILY"
     gene            2608..2883
                     /locus_tag="KPD58_001726"
     CDS             2608..2883
                     /locus_tag="KPD58_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HBG0265497.1"
                     /translation="MNEKLTKILEEAKNDDEFRKELIKTREQRDVMDSFCKVVTSRGY
                     DMTVGELFSMGEEYTSNLLKSVNGGAAYPIEDWSDWYEDFFIMLSNI"
     gene            3513..3824
                     /locus_tag="KPD58_001727"
     CDS             3513..3824
                     /locus_tag="KPD58_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265498.1"
                     /translation="MAFEIIKSIVEAEQTADSIKVKAVTDAESIRADAVNKCESIFAD
                     VKKQAKLMEETLIEKAVTDSRAEVDKILANAKSECLKIEKTAEERKSKAIEAVIGKVV
                     R"
     gene            3826..5751
                     /locus_tag="KPD58_001728"
     CDS             3826..5751
                     /locus_tag="KPD58_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HBG0265499.1"
                     /translation="MAIVNMNKISIVGLEAQKSQILKLLMNRGFVQIDDSAFLTEEEE
                     LKNYLVKDSEESEVIMLEQKMSHVNQAINIVSSLVKQKKSLLAPKREFNKIDSEKEGQ
                     VYAEALELNNLHKEIGTIRSNINLLNNNKSMLEPWVNFDIPLENMETKYTKTILGTVP
                     ISANIDALRVKLEDEIPESVIGIVNSDKLMNYVYLITLKETFDDVLEVLKEFNFSVVS
                     LPNEEGTPLQAIKKYNEMIIRQENVSKDKTEEIKRHADSIHMLENLYDFYTIERDQKK
                     IIERLVKTKTTFCLNGWLPSKRADELIKEITEKFNCCVQTEEGSKEEGFPILLENNKL
                     VTPFESVTNMYSYPSTKDIDPNTILTFFFVVFFGMMLSDAAYGLIIAVVCGFVVYKLK
                     IQKGEGNLIKLIGICGVSTTVWGLIFGGILGDLIPIKALINPLEDVMTLMGMSLLFGI
                     IHIYVGLGIKAYTLIRSGKVIDAIFDIGFWYLCITGVCLLIIPFVAGDIGVFSEVGKY
                     LAIIGAIGLVLTQGRSFKGIPVKLFKGFSSLYGITSYFADILSYTRIMALCLTTGVIA
                     QVINLLGAIAGPILAVVIGVVGHTINLLINALGAYVHTSRLQYVEFFNKFYEGGGVPF
                     VPFKYKTKYTSINKKEM"
     gene            5754..6245
                     /locus_tag="KPD58_001729"
     CDS             5754..6245
                     /locus_tag="KPD58_001729"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HBG0265500.1"
                     /translation="MEFLNALGNGQFLAISGAAIAALFAGMGSAKGTSIAGQAAAGVV
                     TEDPSKFGQLLVLQLLPGTQGLYGFIIAFLILSKAGIIGGDAIPTSAQGLQLLMAGLP
                     IGLVGLVSGIAQGKAAAAGVGIVAKRPEELGKAITFAAIVETYALLGLLVSFLAYNGI
                     AIG"
     gene            6297..6860
                     /locus_tag="KPD58_001730"
     CDS             6297..6860
                     /locus_tag="KPD58_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HBG0265501.1"
                     /translation="MGNEQKMIDRIIADAKQEAQEILDKAKSEADLKVNSANEKAEKE
                     MASYTKLAEAEAEKAASKEISGAYMEAKKQILSKKQEILEEVILEAKNKLLNLKDNEY
                     EEIILNMIEKSNCTDDSEIVLSKKDKKTLKDVLSKKGIKVSDETRDITGGFIVKKGDI
                     EYNYSFEAIIAVEHEYIEQIAAEILFN"
     gene            6875..7852
                     /locus_tag="KPD58_001731"
     CDS             6875..7852
                     /locus_tag="KPD58_001731"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HBG0265502.1"
                     /translation="MAEEKTYPYAVARIRVLEKQLLNRQMFIQMAEAKSPEDSLRIIA
                     EAGYADTSNNNIHNFENILTQELSKTYEILKSLAPEEKFVDVFLYKNDYHNLKVLIKE
                     EISGVDGEKYLIDGGTIPLIKLRESLANRSFSDLPKIMSSAILEAFDVYNKTQNGQMV
                     DIVLDKATFTSMKETAKESQNKFVIDYVMKVCDLTNLKSFIRVKNMKKDFDMFMNVFV
                     SGGSLDKEKFLEAFSSDTPASCFKSTSYSDVCKNGMDSGFTVFEKLCDDYLMEYVRGA
                     KFKPLTLEPLIAYLYAKESEIKTIRIILTSKLNNIDADTIKERLRDAYV"
     gene            7839..8162
                     /locus_tag="KPD58_001732"
     CDS             7839..8162
                     /locus_tag="KPD58_001732"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HBG0265503.1"
                     /translation="MLMYKVGVVGDKDSIMGFLALGIDIFPAYDSDEIKKSIHKLVED
                     EYAIIYITEQASLLAKESIAKYKDYQLPAIIVIPGIGGSMGLGMNEVRESSKRAIGAD
                     ILFKE"
     gene            8279..10057
                     /locus_tag="KPD58_001733"
     CDS             8279..10057
                     /locus_tag="KPD58_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HBG0265504.1"
                     /translation="MKTGTIVKVSGPLVVAEGMRDANMFDVVRVSDKHLIGEIIEMHG
                     DKASIQVYEETSGLGPGEEVVSLGMPMSVELGPGLISTIYDGIQRPLEKMYDISGTNI
                     TKGVEVSSLDRTTKWEFKPKKSVGDKVVAGDIIGGVQETAVVECKIMVPYGVKGTIKE
                     IYSGEFTVEDTVCVITDEKGNDIPVTMMQKWPVRKERPYREKKTPDSLLVTGQRVIDT
                     FFPITKGGVAAIPGPFGSGKTVTQHQLAKWADADIVVYIGCGERGNEMTDVLLEFPEL
                     KDPRTGESLMQRTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYSVALMADSTSRW
                     AEALREMSGRLEEMPGEEGYPAYLGSRLAQFYERAGKVNCLGMDEREGTLTAIGAVSP
                     PGGDTSEPVSQATLRIVKVFWGLDASLAYKRHFPAINWLTSYSLYQEKVDVWADKNVN
                     DNFSAQRAQAMKLLQVESELQEIVQLVGVDSLSDGDRLILEVTRSIREDFLHQNSFHE
                     VDTYTSLHKQYRMMGLILKFYKSAQDAIKQNVTINDIIKLSVLEKIGRAKYVEEGDID
                     AAYDSIEDEIDNELADLIKKRGAEEL"
     gene            10054..11427
                     /locus_tag="KPD58_001734"
     CDS             10054..11427
                     /locus_tag="KPD58_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HBG0265505.1"
                     /translation="MIKEYKSIQEVAGPLMLINGVEGVKFDELGEIELSNGEIRRCKV
                     LEVNGDTALVQLFESSTGINLAESKVRFLGKSLDFGVSPDILGRVFSGMGRPIDGGPE
                     IIPDKRLDINGAPINPAARDYPAEFIQTGVSAIDGLNTLVRGQKLPIFSGSGLPHANL
                     AVQIARQAKVRGTDSKFAVVFAAVGITFEDAEFFISDFKATGAIDRSVVFVNLANDPA
                     VERVSTPRMALTAAEYLAFEQDMHVLVIITDITNYAEALREVSAAKKEVPGRRGYPGY
                     LYTDLATMYERAGKMKGHEGSITMIPILTMPEDDKTHPIPDLTGYITEGQIILSRELY
                     KKDIQPPIDVLPSLSRLKDKGIGKGKTREDHADTMNQLFAAYSRGKDAKELMAILGES
                     ALSEIDLMYAKFADEFEKEYVSQGFRTDRTIEQTLEIGWKLLSMLPKSELKRIRDEYF
                     EKYMPKE"
     gene            11433..12101
                     /locus_tag="KPD58_001735"
     CDS             11433..12101
                     /locus_tag="KPD58_001735"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HBG0265506.1"
                     /translation="MARLNINPTRMEMTRLKKLLKTATRGHKLLKDKLDELMKQFLEI
                     VRENKRLREEAENALDTAYKNFIIARAVMSQEYLGSALMMPKQSVSVDVSTRNIMSVD
                     VPVFDFKTENNQSDIYPYGLAFTSGELDSAMEAFSDAMQPLLRLAESEKSAQLLAQEI
                     EKTRRRVNALENVMIPNYIETIKYIAMKLEENERASTTRLMKVKDMVLKKALEEKKKN
                     DLVV"
     gene            12378..13427
                     /locus_tag="KPD58_001736"
     CDS             12378..13427
                     /locus_tag="KPD58_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG0265507.1"
                     /translation="MKEFIFTCLCIVVITFMTSCISTTPQTTQGKGNNKLTIYAALEE
                     EQIAYYMEGFREEYPNIDVEIVMDSHGVIASKLLAEKDNPQADIIWGLSAMNMIELKK
                     DGALEAYKPKNYDKVDKKFKDTEDDVSWVGMSVPETAIVVNYKELKKLGIDIPKSYED
                     LIKPEYKGLITMPNPASSGTGYLTVSGLIQMMGEKDAYNYMDKLHKNIATYTHSGSKP
                     AKLAGSGEYPIGITLGYRATQQLSSGEPIEVVFPKEGSGWDLEANALVKKDNIKKEAK
                     LFLDWAISDSAMDRYAHEVAVTSIKTDNPVPKGYSKQPFEQLIKEVDLNSAAKNRSTV
                     LKTWESKYGSKSESK"
     gene            13523..14512
                     /locus_tag="KPD58_001737"
     CDS             13523..14512
                     /locus_tag="KPD58_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0265508.1"
                     /translation="MSYLKINNVFKSYDQKRVLNNISLDIEEGEFLCLLGPSGCGKTT
                     LLRIIAGLEDVNSGTIILQDKDITNLEPSKRGFGIVFQSYALFPNMTAYNNIAFPLKE
                     RKVSKGKIDNKVKEVLETVGLTNEAHKYPKALSGGQQQRIAIARALALEPKFLLLDEP
                     MSALDAKVRHKLRMDIKRLQKELNITTIMVTHDQEEAITMADKIAILNGGDIMQIGTP
                     EEIYQNPQNLFTAQFIGDTNCFDNGDSILTVRPEYVQIEKSTKENYQGIISNIEFRGN
                     LLRVEIKDKLNENFIISDASIKEWVNLNLVEGDLVKISIDEKYYLKYPKVKGA"
     gene            14517..16235
                     /locus_tag="KPD58_001738"
     CDS             14517..16235
                     /locus_tag="KPD58_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HBG0265509.1"
                     /translation="MNNNAISSKSISLKSKMNLDIKKCMLTVLVMSLVIFLLAPIIYL
                     FVQAFLDKDGVFVGLQNFQEYLASESLLNSLKNTVFVSVISTVISLTLALVYAYALTR
                     TKIKFKGFFKSLAMFPMFAPTMMHAIGLVYLFGNQGLVTNLLGVHVPIYGAVGIILAQ
                     IIYVFPQSFQILYISLSSTDYRLYEASNTMGISKIREFISITIPSIKYSLISSAFVSF
                     TLCFTDFGAPKVIGGEFNVLSIDVYKQIIGQQNMPMGATVSILLLIPAIISFIVTKKI
                     EKNQNSYITSKSIPYKVKTNKLRDSILGGVVGIIAFGIIAVMLTVILSSLISVWPYDL
                     RLTLEHFSMKNSIDGIKPFINSIKMSLLTAILGTSFVFLFAYLNEKTEKAPYLKSFGY
                     FLSTLPMALPGLVLGIAYVLFFNKLEFNFMDTIYIKNLFNGLYGTLLIMVICNIVHFF
                     SVAFITATTAIKKVDKELDMVAKSMGINSASILKNVTIPLCLPAVLENFMYFFLNSMV
                     TISALVFIYTASSKVAAISIVQLDDKGSTAQAASLAVLILVTNILVKVIYEFIKNRLE
                     KRTNFN"
     gene            complement(16317..>16473)
                     /locus_tag="KPD58_001739"
     CDS             complement(16317..>16473)
                     /locus_tag="KPD58_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG0265510.1"
                     /translation="VEENSTNELKLSMSIGYAIFDEKVDVSLFDTYRRADSQMYDNKR
                     KINKQYQ"
BASE COUNT         6338 a         2006 c         3077 g         5052 t
ORIGIN      
        1 tagatgataa aaactttaat aaaaatttag tgtataggag ttgtttaagt atgatagata
       61 taagtattga tgacaaactt aaggaaagat ggcctaatgc taagctgggt tgcattcaag
      121 caaaagtagc agtaaataaa agctcagaaa aattaattaa tgaaataaat gagttttgtg
      181 atactataaa tcaaagtcta aaagttgaag acattacgaa actagataaa attaaagatg
      241 caagaaatgc ttataaagag cttggaaaaa atccaagtag atatagaaca tcttcagaag
      301 cattggttag aagaattatt caagggaagg gtctttatac aataaataat atagtagaaa
      361 taaacaattt aatatcaata aaatcattat atccagtagg tacatataat gtaagcaaat
      421 tacattcacc agtatgtttt actgtaggag atgaaggaga acagtataag ggtattggga
      481 aagaattaat aaatatagaa aatcttccta tattatctga ttcagtgggt aaatttggga
      541 gtccaactag tgattctgaa agagcaatga taacagaaga tgcaaatgaa atattaattt
      601 gtatattttc ttttagtcaa gaatctgata ttgaagaata ttttggatat gctaaggaat
      661 tacttaaaga atatgcagat ggaaaagata tagaaactaa aataattaaa tagaatctat
      721 ataacaacgt tatgttaagc taaaattcaa tataaaaata agttaagata aagaaactaa
      781 cacaaaatta gtttcttttt taaaatagta gtttagtgtt acaaaaatag atagatagtc
      841 ccacgattat taaaagtatg gtaatatatt aatatagcag agattgtgtt taagggattt
      901 tgaaaggggg gatttataat aatcatttta tctaaaagat aaaatggttt tatagaaata
      961 actatatatt taaaaatata aataatgata cagtaatttg ggtatataaa ataaggcata
     1021 catggttttg tttaaaaacc aataaggtac ataggtaatt acaatgtaag gaaggattta
     1081 attatgattg atggaaaaga ggtaaaacaa gaaagtggac aaaaaaatct aaaatttaag
     1141 atagctatag tagtaggtgt tttgtttgta gcttatatag gaatggcaat cttttttaga
     1201 aatcattttt actttggaac gactattaat ggcgtaaagg cttcaggaaa aacagttgag
     1261 caagtaaatg atttgttaac atctagtact aattcctata ctttaaactt agaggaaaga
     1321 aacaataaga aagaacaaat aaaagcaaaa gatattgatt taaaatttac accagatgat
     1381 aaagttcaaa agataaaaga tagtcagagc gctattggat ggattggtgg aatatttaag
     1441 tctaaagagt ataataatat gataactctt tcatataata aagatttact agaaaaaagg
     1501 tttaatgctt tatcttgttt tgatagtaaa aatgttgtag aacctaagag tgcttatcct
     1561 aagtatgata gcactaaaaa cgcttatgtt atagtaccag aggtacttgg aaacaaagtg
     1621 aaaaaagatg atttttataa gttagtacaa aactcaatta attctgggaa tacttctatt
     1681 tctctggatg aaagtaaagt ttatgaagca ccaaacaaca caaaggattc agaaaagttg
     1741 aaacaagcta ttaagacatt agataagtat gttggtgtag tcgttacata tgattttagt
     1801 gaccgtaagg aaactttaga tggttctgaa attaataaat ggttaaaagt agactcagaa
     1861 aagaattatg acataacttt tgataatgat gctatggtag aatatacaag aggtttaagt
     1921 aaaaaataca gtacttttgg tgactcaaga ccatttaaaa ctgccacagg taaagatatt
     1981 acaatttcag gtggaattta tggttggttg atagataaaa agaaagaagc tgaagcatta
     2041 gttgatgttg taaaagctgg taaaaatgtt acaagagaac ctatttacat acaaaaagct
     2101 gtatctcgta ataaagatga tattggaaat acatatgtag agattagtct ttctggccaa
     2161 catatgtggt tctttaaaaa tggagaaatg cttgtatcaa cagacgttgt aacaggtgat
     2221 ttagctagag gatttgctac accatcaggt atatatcctc ttaattataa ggctagaaat
     2281 gtttcattaa ctggtcaagg ttacagttca cctgtaagtt attggcttcc gtttaatggg
     2341 aacataggca ttcatgatgc tacttggaga aattcttttg gtggaagtat atataagtct
     2401 tcaggttcac atggatgtgt aaatacacct ttttctaagg caaagaaaat atatgaaaat
     2461 atagaaccag gtacaccaat tattttatac tagtaaatgt gttgctaaca gttaagcgaa
     2521 gtcatcttag tttatttagg atggcttcct ttttatataa agcttattga agtttacaga
     2581 aagactactt aaaaggagaa atacataatg aatgaaaaat taactaaaat tttagaagaa
     2641 gctaagaatg atgatgaatt tagaaaagaa cttataaaaa caagagaaca acgtgatgtt
     2701 atggattcat tttgtaaggt tgttacatct agaggttatg atatgacagt aggggaactt
     2761 ttttctatgg gagaagaata tacatccaat ctactgaaaa gtgtaaatgg aggagcagca
     2821 tatcctattg aagattggag tgattggtac gaggattttt ttataatgtt gtcgaatata
     2881 tagagtaact aatgatatta tctacttaag attaatacat tgccttaaaa taaaactatt
     2941 agaactaggt gtaagtttta tcaatcttta aaaatattga tttatttcta actagatata
     3001 aaaaaattag attaaaaata aggtgacaaa aatcgatata gcttgatgat ttataaaagt
     3061 tagataaaat agtgcgagat atttctaaaa attcaaaaaa aacaataaac ttcaatggtt
     3121 agaggttgaa aaagaatata aaaaactttt atatgtaata aattaaatta aaattataaa
     3181 aaaaataaaa taaaaattta aaatgtcgac ataacgttaa ttttaaaaca aaataacgct
     3241 attgacaaac tatatcgctt taaattataa ttattgaaaa taatggtatt taaaaaccat
     3301 actcatgtca tagtctaaaa gtaagtcaaa aaaggatgta aatctaggca agtactattc
     3361 taaaatatgt tttattaaga aataaacgtt gaaaaatatt aattattata taaaaatgct
     3421 gtttatactt taacaatgag gtagtaatct aattagaata aaaaaatcta attggttata
     3481 tacatagtaa aaaggtaaga gaggtgattt ggatggcttt tgaaataata aaaagcattg
     3541 ttgaggcaga gcagacagca gacagtatca aagtaaaagc tgttactgat gcagagtcta
     3601 tcagagctga tgctgtaaac aaatgtgaaa gcatatttgc tgatgtaaaa aaacaagcaa
     3661 agcttatgga agaaactctt attgagaagg cagtcaccga cagtagagca gaggttgata
     3721 aaatcttagc taatgctaaa agtgaatgtc tgaaaattga aaaaactgct gaagaaagaa
     3781 aaagtaaggc tattgaagcg gttattggaa aggtagtgag ataatatggc aatagttaat
     3841 atgaacaaaa tatctattgt tggcttagaa gctcaaaaaa gccagatttt aaagctactt
     3901 atgaaccgtg ggtttgttca gattgatgat agtgcctttc ttactgagga ggaggaatta
     3961 aaaaattatc ttgtaaaaga tagtgaagaa tctgaagtta taatgctaga gcagaaaatg
     4021 tctcatgtaa accaagcaat taatattgtt tcttcactag tgaaacaaaa aaaatcccta
     4081 cttgcaccaa aaagagagtt taacaaaatt gatagtgaaa aagaagggca agtttatgct
     4141 gaagctctgg agttaaacaa tttgcacaag gagataggta caataaggtc taacataaac
     4201 ttacttaaca acaataaaag tatgttagaa ccatgggtga actttgatat tcctcttgaa
     4261 aatatggaaa ctaagtatac aaaaactata cttggaactg tgcccataag cgcaaacatt
     4321 gatgctttaa gagtaaaatt agaagatgaa ataccagaaa gtgttattgg gattgtcaat
     4381 tctgacaagt taatgaacta tgtttattta attactttaa aagaaacttt tgatgatgtt
     4441 ttagaagttt taaaagaatt taatttttca gtggtttcat taccaaatga agaaggtaca
     4501 ccgttacaag ctatcaagaa atacaatgaa atgataatta gacaggaaaa cgtttctaag
     4561 gacaaaacag aggaaattaa aagacatgca gatagtatac acatgcttga aaacctatat
     4621 gatttttaca ctatagaaag agaccaaaaa aagattatag agagacttgt caaaacaaaa
     4681 acaacattct gtcttaatgg ttggttacca agcaaaaggg cagatgaatt aattaaagaa
     4741 attacagaaa agtttaattg ttgtgttcag actgaagaag gaagtaaaga agaaggcttc
     4801 ccaattctgc ttgaaaacaa taaattagta acaccttttg aaagtgtaac taacatgtac
     4861 agctatccaa gtacaaaaga tattgaccca aatacaattt taactttctt ctttgttgta
     4921 ttctttggaa tgatgctaag tgacgccgca tatgggttaa taattgcagt agtctgtgga
     4981 tttgttgtat ataaattaaa gatacaaaaa ggtgaaggaa atttaattaa acttattggt
     5041 atctgtggag tttcaactac tgtatggggt cttatattcg ggggtatttt gggggattta
     5101 attcctataa aagcattaat taatccttta gaggatgtta tgacacttat gggaatgtcg
     5161 ttactgttcg gtattatcca tatatatgta ggtttgggaa taaaagcata tacattaata
     5221 agaagtggaa aggtaataga tgcaatcttt gacataggtt tttggtattt gtgtataaca
     5281 ggtgtatgtc tacttataat accttttgtt gcaggggata taggtgtatt ttcagaagta
     5341 ggaaaatatt tagcaattat aggtgctata ggtcttgtac ttactcaagg aagaagcttt
     5401 aaaggtattc ctgttaagct ttttaaagga ttttcaagtc tctatggtat aacaagttat
     5461 tttgctgata tattatctta tacaagaatt atggcgcttt gtttaacaac aggagttata
     5521 gcacaagtta tcaacctttt aggagctata gctggtccga tacttgcagt tgtaattggt
     5581 gttgtaggac acacaataaa ccttcttata aatgcactag gtgcatatgt tcatacaagt
     5641 aggttacagt atgtagaatt tttcaataag ttttatgaag gcggaggagt tccttttgtt
     5701 ccgtttaagt ataaaacaaa atatacaagc attaataaaa aggagatgta aatatggaat
     5761 ttttaaacgc gttaggaaat ggtcaatttt tggcaatatc aggagctgct atagcagcat
     5821 tatttgcagg tatgggttct gctaaaggta cttcaatagc aggtcaagcc gcagcaggtg
     5881 ttgtaacaga agaccctagt aaattcggtc aacttctagt tttacaactt cttcctggta
     5941 cacaaggtct gtatggattt atcatagctt tccttatttt atcaaaagca ggaattattg
     6001 gtggagatgc aataccaaca tcagctcaag gtttacaact tcttatggca ggacttccaa
     6061 ttggtttagt tggtttagta tcaggtatag cacaaggtaa agcagcagca gcaggagttg
     6121 gaatagttgc aaaaagacct gaagaacttg gtaaagctat aacatttgca gcaatagttg
     6181 aaacatatgc tcttttagga ctacttgttt ctttcttagc ttacaatgga attgcaattg
     6241 gttaattata aattataatt cataagtaaa aaaaatgaaa aggaggaagc actcctatgg
     6301 gtaatgaaca aaaaatgatt gacagaatta tagctgatgc caaacaagaa gcacaagaaa
     6361 tattggataa agctaaaagt gaagcagatt taaaagtaaa ttcagctaat gaaaaagctg
     6421 aaaaggaaat ggcttcctat acaaagcttg cagaggcaga agcagaaaaa gcggcttcta
     6481 aggaaatatc aggagcttat atggaggcta aaaagcaaat attatcaaaa aaacaagaga
     6541 ttttagaaga ggtaatttta gaagctaaaa acaaactttt aaatcttaaa gataatgaat
     6601 atgaagagat tatactaaac atgattgaaa aatcaaattg tacagatgat tcagagatag
     6661 tattatctaa aaaagataaa aaaacattaa aagatgtgct atcaaaaaaa ggcattaaag
     6721 tgtctgatga aactagagat attactggag gtttcatagt taaaaaaggt gacattgagt
     6781 acaattattc atttgaagcc ataattgcag tagagcatga atatattgaa caaattgcag
     6841 cagaaatttt gtttaattaa ggaggggtta aaaaatggca gaagaaaaaa cttatcccta
     6901 tgccgttgca cgaataagag tgcttgaaaa acagcttctt aacagacaaa tgtttattca
     6961 aatggcagaa gccaaaagcc ctgaagactc tttaagaatt atagctgagg caggttatgc
     7021 tgatacatca aataataata tacataattt tgagaatatt ttgactcaag aattatcaaa
     7081 gacatatgaa attcttaaaa gtctagctcc tgaagaaaaa tttgttgatg tatttcttta
     7141 taaaaatgac tatcacaatt taaaagtgct gattaaagaa gaaatttctg gtgtagatgg
     7201 tgagaaatat ttaattgatg gaggtacaat tcctcttatt aagcttagag aatctttagc
     7261 aaatcgtagt tttagtgatt tacctaagat aatgtcatca gctatattag aggcatttga
     7321 tgtttataat aagacacaaa atggccagat ggttgatatt gttctagata aagcaacctt
     7381 taccagtatg aaagaaactg ctaaagaaag ccaaaacaaa tttgtaatag attatgttat
     7441 gaaagtttgt gatttaacaa atctaaagag ttttataaga gtcaaaaata tgaagaaaga
     7501 ttttgatatg tttatgaatg tgtttgtttc aggtggctca cttgataagg aaaaattctt
     7561 agaagctttt tcctcagata ctccagcatc atgttttaaa tctacttctt actctgatgt
     7621 ctgtaaaaat ggcatggata gtggttttac tgtctttgaa aaactttgtg atgactatct
     7681 tatggaatat gttagaggag caaaatttaa gcctcttaca ttagaaccac taattgcata
     7741 tttatatgct aaagaatcag agataaaaac aattagaatt attttgacta gtaagcttaa
     7801 taatatagac gcagatacta taaaagaaag gctaagggat gcttatgtat aaggtaggag
     7861 tagttggaga taaagatagc atcatgggat ttttagctct tggaatagat atcttcccag
     7921 cctatgatag tgacgaaatt aaaaaaagta tacataagtt ggttgaagat gaatatgcga
     7981 taatttatat tacagaacag gcatcattgc ttgcaaaaga atcaatagca aaatacaaag
     8041 attaccaact tcctgccatt atagttattc caggtattgg aggaagtatg ggattaggta
     8101 tgaatgaagt aagagaatct agtaaacgtg ctataggtgc agatatatta tttaaagaat
     8161 agatgaaagt tgaataaaag acaatctcaa attttattgg tgctaaatat atgtgctaat
     8221 aaaatatagg agggaatatt taaattttga taaagtatat gattgcaggt gagagaatat
     8281 gaaaacaggc acaattgtaa aggtgtcagg accgcttgtt gtagcggagg gcatgagaga
     8341 tgcgaatatg tttgacgtcg taagagtttc agataaacat cttataggcg agattataga
     8401 aatgcatgga gataaagctt ctatacaggt ttatgaggaa acttccggtc taggaccagg
     8461 ggaagaagta gtttctcttg gaatgcctat gagtgttgaa cttggaccag gtcttatttc
     8521 aactatttat gatggtattc aacgtccact tgaaaaaatg tatgatattt caggtactaa
     8581 tataactaaa ggtgttgaag tatcttcact tgatagaaca actaagtggg aatttaaacc
     8641 taaaaaatct gttggtgata aggttgtagc aggagatata attggtggcg ttcaagaaac
     8701 agcagtagta gaatgtaaaa ttatggttcc ttatggagtt aagggtacta taaaagaaat
     8761 atattctggt gaatttactg ttgaagatac agtttgtgtt attacagatg aaaaaggtaa
     8821 tgatatacct gtaactatga tgcaaaaatg gccagttaga aaagaaagac catatagaga
     8881 aaagaaaaca ccagattcac ttcttgttac aggtcaaaga gttatagata catttttccc
     8941 aatcacaaaa ggtggggtag cagctatacc gggacctttt ggaagtggaa aaactgttac
     9001 acagcatcag cttgctaaat gggcagatgc agatatagtt gtatatatag gctgtggcga
     9061 gcgtggaaat gaaatgacag atgttcttct tgaatttcct gaattaaaag acccaagaac
     9121 aggcgagtca cttatgcaaa gaactgtgct tatagcaaat acatcagata tgccggttgc
     9181 tgcacgtgaa gcttctatat acactggtat tacaatagct gaatatttta gagatatggg
     9241 atatagtgtt gcacttatgg cagactctac atcaagatgg gctgaggctc ttagagagat
     9301 gagtggtcgt ttagaggaga tgcctggtga agaaggttat cctgcatact taggttcacg
     9361 tcttgctcaa ttctatgaga gagcaggaaa ggtaaattgt ctaggtatgg atgaaagaga
     9421 aggaacactt acagcaattg gtgcagtttc tcctcctggt ggtgatacat cagaacctgt
     9481 cagccaagca actcttcgta tagtaaaagt attctggggt cttgatgcat ctttagcata
     9541 taaacgtcat ttcccagcaa ttaactggtt gactagttat tcactctatc aggaaaaagt
     9601 tgatgtatgg gcagataaaa atgtaaatga taatttctca gctcaaagag ctcaagctat
     9661 gaaactttta caagttgaat ctgaacttca agaaatagtt caattggttg gggttgattc
     9721 actttcagat ggagatagat tgatactaga ggttactcgt tctataagag aagacttcct
     9781 tcatcaaaac tctttccatg aagttgatac ttatacatca cttcataaac aatatagaat
     9841 gatgggactt atattaaaat tctataagtc agcacaagat gctatcaagc aaaatgttac
     9901 tataaacgat attatcaagc tttcagttct tgaaaagatt ggtcgtgcta agtatgtaga
     9961 agaaggcgat atagatgcag cttacgattc aattgaagat gaaatagaca acgagcttgc
    10021 agaccttatt aaaaagaggg gggctgaaga actatgataa aggaatataa gtcaatacaa
    10081 gaggtagccg gtcctcttat gttaataaac ggcgtagaag gtgtaaaatt tgatgaactt
    10141 ggagaaattg aactttcaaa tggagaaatt cgccgttgca aagttctaga ggtaaatgga
    10201 gatactgcac ttgttcagct tttcgaaagc tcaacaggta taaaccttgc tgaaagtaaa
    10261 gttcgttttt taggaaagag tcttgacttt ggagtttcac cagatatatt aggtcgtgtt
    10321 tttagtggaa tgggtagacc tattgatgga ggtccagaaa ttataccaga taaaagactt
    10381 gatataaatg gagctccaat aaatccagca gcacgtgatt atccggccga gtttatacaa
    10441 actggagtct ctgctataga tggacttaat acattggtta gagggcaaaa acttcctata
    10501 ttctcagggt ctggtttgcc acatgccaat cttgcagttc agattgcaag acaggcaaaa
    10561 gtacgtggaa ctgattctaa gttcgccgtt gtatttgctg cagttggtat aacttttgaa
    10621 gatgctgaat tttttataag cgattttaaa gcaactggtg caatcgaccg ttctgtagtg
    10681 tttgtaaacc ttgcaaatga cccagctgta gagcgtgtat ctacacctag aatggcactt
    10741 acagcagcag aataccttgc tttcgagcaa gatatgcatg tacttgttat aataacggat
    10801 attacaaact atgcagaggc gttgcgtgaa gtttcagcag ctaaaaaaga agttccagga
    10861 cgtcgtggat atccaggata tctttatact gaccttgcta ctatgtatga aagagcagga
    10921 aaaatgaaag ggcatgaagg gtctataact atgattccaa tacttacaat gcctgaggat
    10981 gataagacac atccaattcc tgaccttaca ggatacatta cagaagggca aattatactt
    11041 agccgtgaac tttataaaaa agatatacag cctcctatag atgttttacc atcactttct
    11101 cgtctaaaag ataaaggtat tggtaaaggt aagactcgtg aagaccatgc agatacaatg
    11161 aaccagcttt ttgcagctta ttcaagaggt aaggatgcaa aagaacttat ggctatactt
    11221 ggtgaatctg cactttctga gattgattta atgtatgcta agtttgcaga tgaatttgag
    11281 aaagaatatg tttcacaagg atttagaact gatagaacta ttgagcagac attagaaatc
    11341 ggatggaagc ttcttagtat gttaccaaaa agtgaactta aacgtatccg tgatgaatat
    11401 tttgaaaaat atatgcctaa ggagtgatat ctgtggctag attaaatata aatccaacac
    11461 gtatggaaat gactagactt aagaaactcc ttaaaactgc tacaagaggt cataagcttc
    11521 ttaaagataa acttgatgaa cttatgaaac aatttcttga gattgtaaga gaaaataagc
    11581 gattgcgtga agaagcagaa aatgctcttg atactgctta taaaaacttt ataatcgcaa
    11641 gagcagttat gagtcaggag tatctgggtt cagcacttat gatgccaaag caatcagttt
    11701 cagtagatgt ttctacgaga aatataatga gtgttgatgt tcctgtgttt gactttaaaa
    11761 ctgaaaacaa tcaaagtgat atttatcctt atggtcttgc ttttacatca ggtgaattag
    11821 attctgctat ggaagctttc tcagatgcta tgcaaccact tttaagactt gcagaaagtg
    11881 aaaaaagtgc acagcttctt gcacaagaga tagaaaagac acgtagacgt gttaatgctc
    11941 ttgaaaatgt aatgatacca aattacattg agactattaa gtatatagct atgaagcttg
    12001 aagaaaatga gagagcaagt actactagac ttatgaaggt taaggatatg gtgcttaaaa
    12061 aagcacttga agaaaagaaa aaaaatgatt tggttgtata attaaatact tataaaaata
    12121 agtctaacat tctactttat agaggttaga cttatttttt atgtttaaat taaaatgtag
    12181 atactataaa aattaatata ttgtttaaaa acagactgta tttaactaaa aataatatat
    12241 ttaacaaaaa tttctaaaaa ttaacgaact gttaatttac acttaacttt atattaacaa
    12301 attaggtgta ttatataaac ataagcaaaa acaataaaaa acaaataaaa aaaggaaaaa
    12361 acacaaaggg ggaagatatg aaagaattta tatttacttg tctgtgcatt gtagtgatta
    12421 catttatgac aagttgcata tcaacaacac cacaaactac tcaaggaaaa ggaaataata
    12481 aacttacaat atatgcagca ctagaggaag agcagatagc ttattatatg gaaggtttta
    12541 gagaagagta tcccaatata gatgtagaga tagttatgga ttctcatggt gtaatagctt
    12601 caaaactatt agcagaaaaa gataatccac aagcagatat tatatgggga ttatcagcaa
    12661 tgaacatgat agagctaaaa aaagatggtg cacttgaagc atataagcct aaaaattatg
    12721 ataaagttga taaaaaattt aaagatactg aagatgatgt atcttgggta ggaatgagtg
    12781 tcccagagac agcaatagta gttaattata aggaacttaa gaaactaggt atagatattc
    12841 caaaaagtta tgaagattta ataaaaccag aatataaagg acttataaca atgccaaatc
    12901 cagcatcatc tggaacgggg tatcttacag tatcagggct tattcaaatg atgggtgaaa
    12961 aagatgctta caattacatg gataaattac ataaaaatat agccacatat acacactcgg
    13021 gttcaaaacc tgcgaagtta gctggctctg gagagtatcc aataggtatt acacttggat
    13081 atagagctac acaacaatta tctagtggag aacctataga ggttgtattt ccaaaggaag
    13141 gttcaggctg ggacttagaa gcaaatgcct tagttaaaaa agataatata aaaaaagaag
    13201 caaagctatt tttagattgg gcaataagtg attcagctat ggatagatat gcacatgaag
    13261 tagcagttac ttccataaag acagataatc cagttccaaa aggttattct aaacaaccat
    13321 ttgaacagct tataaaagaa gttgatttaa actctgctgc caaaaacaga agtacagtac
    13381 ttaaaacttg ggaatcaaaa tacggttcaa aatctgaaag taagtaaagt ttaaattaat
    13441 aatttagatg aaagaatttt agaagaaaaa gtaggatata aaatttaaaa taaaaaaata
    13501 actatataag gagagataag atatgagtta cttaaaaata aataatgtgt ttaaaagtta
    13561 tgaccaaaaa agggttttaa ataatattag tttggatatt gaagaaggag aatttctatg
    13621 tttactcgga cctagtggat gtggtaaaac tacattactt agaataatag caggtcttga
    13681 agatgtaaat agcggaacta taattctcca ggataaagat attacaaatt tagagccatc
    13741 aaagagaggt tttggtatag tatttcaatc atatgcatta tttccgaata tgactgctta
    13801 taacaatata gcttttccac taaaagagag aaaagtttca aaaggaaaaa tagataataa
    13861 agttaaggaa gtacttgaaa cagttggatt gactaatgaa gctcataaat atcctaaagc
    13921 attatcaggt gggcaacaac agaggattgc aatagcaaga gcattagctc ttgaaccaaa
    13981 gtttttactt ttagatgaac caatgtccgc attagatgca aaggttagac acaagcttag
    14041 aatggacatt aaaagattac aaaaagaact taatataacg acaataatgg taacacatga
    14101 ccaagaagaa gctataacta tggcagataa aatagctatt ctaaatggag gagatataat
    14161 gcaaataggg actccagaag aaatatacca aaatcctcaa aacctattta cagctcaatt
    14221 tataggagat acaaactgtt ttgataatgg agattctata ttaactgtaa gaccagaata
    14281 tgttcaaata gagaaatcga caaaagaaaa ctaccaagga ataatatcaa atatagagtt
    14341 tagagggaat ttattgagag ttgaaataaa agataaatta aatgaaaatt tcataataag
    14401 tgatgcttct ataaaagagt gggttaattt aaatttagta gaaggtgacc ttgtaaaaat
    14461 aagtatagac gaaaaatact atttaaagta tccaaaagtt aaaggagcat aagtgtatga
    14521 ataataatgc tataagttct aaaagtataa gtcttaaatc aaaaatgaat ttagatataa
    14581 aaaaatgtat gcttacagta ttagttatgt ctttagtaat atttttatta gcacctataa
    14641 tatatctatt tgtacaggct tttttagata aagatggagt ctttgtagga ttacaaaact
    14701 ttcaagaata cttggcttct gaatctctat taaattctct aaaaaataca gtatttgtat
    14761 cagttatatc aactgtaata tcactaacac ttgcattagt atatgcatat gcattaacta
    14821 gaacaaagat taaatttaaa ggttttttta aatcattagc tatgtttcct atgtttgctc
    14881 caacaatgat gcatgcaata ggtctagtat atttatttgg aaatcaaggt ttggtaacta
    14941 atttacttgg agtgcatgtt ccaatatatg gagcagtggg aataatattg gcacaaatta
    15001 tatatgtatt tcctcaatca tttcaaatac tttatatatc tctaagttca accgattata
    15061 gactatatga agctagtaat actatgggga taagcaagat aagagaattt ataagcataa
    15121 caataccaag tataaaatac tctttaataa gttctgcatt cgtatcgttt acattatgtt
    15181 ttacagattt tggagctcca aaggtgatag gaggagagtt taatgtatta tcaatagatg
    15241 tatataagca aattataggg caacagaata tgccaatggg agcaactgta tcaatattac
    15301 ttttaatacc tgcaataata tcgtttatag taactaaaaa aattgaaaaa aatcaaaatt
    15361 catatataac ttcaaagtca attccatata aggttaaaac taataagtta agagatagta
    15421 ttttaggagg ggttgtagga attattgcat ttggtataat agctgtaatg ttaactgtaa
    15481 tcttatcttc attaatatca gtttggccat atgatttaag actgacactg gaacattttt
    15541 ctatgaaaaa cagtattgat gggattaaac catttataaa ttcaataaaa atgtcactat
    15601 tgacagctat tctagggacg agctttgttt tcttatttgc atatttgaat gaaaaaacag
    15661 aaaaagctcc atatttaaaa tcatttgggt attttttaag tacattgcct atggcattgc
    15721 caggattagt tttaggtata gcttatgtat tattttttaa taaattagag tttaatttta
    15781 tggatacaat atacattaag aatttgttta atggattata tggaacatta cttataatgg
    15841 ttatatgtaa tatagttcat ttcttttcag tagcatttat aacagctact actgctatta
    15901 agaaagtaga taaagaattg gatatggtag ctaaatcaat gggaataaac agcgcatcaa
    15961 ttcttaagaa cgtaactata ccactttgtt tgccagcagt tttagagaac tttatgtact
    16021 ttttcctaaa ctccatggtg acaatatcag cattagtttt tatatatact gcaagttcaa
    16081 aagtagctgc aatatcaatt gtacaactag atgataaggg gagtactgca caggcagcat
    16141 cactagctgt gttaatatta gtcacaaata tattagtgaa agtaatatat gaatttataa
    16201 agaatcgttt agaaaaaaga acaaacttta attaaataat ttatcttata aaaacacaaa
    16261 tttaaaaaag tctattcttg tgatttatta tttaaagaat agacttttta aatttattat
    16321 tgatattgtt tattaatttt ccttttgtta tcatacattt gagaatctgc acgacgatat
    16381 gtatcaaata aagaaacatc tactttttca tcaaaaatag catatccaat agacattgat
    16441 aattttaatt cattagttga gttttcttca act
//
LOCUS       DAELLV010000067        16418 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.067, whole genome shotgun sequence.
ACCESSION   DAELLV010000067 DAELLV010000000
VERSION     DAELLV010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16418
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.067"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..474
                     /locus_tag="KPD58_001740"
     CDS             <1..474
                     /locus_tag="KPD58_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG0265511.1"
                     /translation="LQRELSRKTIGSLNRNKARLKVARLQEHIANQRNDFLQKLSTKL
                     IKENDIICIEDLQVKNMIRNRKLSRSISDVSWSEFIRQLEYKANWHGRQIVKVGKFFA
                     SSQICNKCGYKNEEVKDLNIREWIYPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            906..1160
                     /locus_tag="KPD58_001741"
     CDS             906..1160
                     /locus_tag="KPD58_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265512.1"
                     /translation="MENKDIKLTICIFAFVIGIIVLFCCIGLGQFNMDCIIKQNGGSI
                     ATEQYNIYLEQSISQYRNFGSILVLLGGIGILFDKSVRKY"
     gene            1196..1519
                     /locus_tag="KPD58_001742"
     CDS             1196..1519
                     /locus_tag="KPD58_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265513.1"
                     /translation="MARDVFSVKMTPLEAQKMVEKNLEADLVSVDIYNLENNKSIIVT
                     IFEKYYARSNSDAGLVVICENTTGKTLVKITSTGSAIGLFQIDWGTGNNLIKRVKKIL
                     IDYII"
     gene            1793..1945
                     /locus_tag="KPD58_001743"
     CDS             1793..1945
                     /locus_tag="KPD58_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265514.1"
                     /translation="MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINF
                     KYHRKK"
     gene            2139..3062
                     /locus_tag="KPD58_001744"
     CDS             2139..3062
                     /locus_tag="KPD58_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HBG0265515.1"
                     /translation="MVGAFVLPNYAIILSEMSNLWGNFIMWILVPIITIVLLIIAVSS
                     MQYILVMIAFLLIIYSFIEKKIVMGLVSVLFFTYSIYLCATWEDKSLIADNKVETVRA
                     QRETVEREKEMERRRIQEEVDKERYIEKHGMEISENDLKVKLEALVPQEYKGKKYELK
                     VGKFKRYSMYFDLTVQNEKFSNSEECKKFVKEIANDLKKIKISKAYFKFHSKDDGGIY
                     NSVYIDYFRNIQNNVDNVENLEFNEFELKTEEEEKREQEKIEQEKNSYNNYIQNRVVD
                     PLDRIKKLKELLDSGAITQEEYNKKKKELLE"
     repeat_region   3329..4408
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3329..3357
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaat"
     gene            4599..4751
                     /locus_tag="KPD58_001745"
     CDS             4599..4751
                     /locus_tag="KPD58_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265516.1"
                     /translation="MKNRRKDKRGFSVLLDKEKLDKFDEVLEEKNLTKKEWLEEKIDE
                     ELEQKE"
     gene            4872..5685
                     /locus_tag="KPD58_001746"
                     /pseudo
     CDS             4872..5685
                     /locus_tag="KPD58_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888845.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin Bro"
     gene            5737..5898
                     /locus_tag="KPD58_001747"
     CDS             5737..5898
                     /locus_tag="KPD58_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265517.1"
                     /translation="MCENLLDNMYIEKRKEEYRIKLLKIRETDIDIYNKLESIVYKLS
                     EKKLEKNNK"
     gene            complement(6227..6691)
                     /locus_tag="KPD58_001748"
     CDS             complement(6227..6691)
                     /locus_tag="KPD58_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265518.1"
                     /translation="MDFGEVKSELSDKDLKDNEEALNKYKKYYLKVYKYYSEMDSIKS
                     IQDIEIEQSRLKGQLQKNSNPIFALYISIMAVVFSTTINLTFDLSDITNYFIRMIMTL
                     LFIVLLIFLVNKGILPIAIPYRNNIQFYTTALDVLEDMKKKILRERKYKKRR"
     gene            complement(6827..7144)
                     /locus_tag="KPD58_001749"
     CDS             complement(6827..7144)
                     /locus_tag="KPD58_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265519.1"
                     /translation="MDFGNINLILIGIIVIIGTTIIYLIKPKTAFCSKKYFNKLESIY
                     GNIDKKKTVKLEVLYRYVTGLEYIAIGLFTRRLDITILAIILVAIITTALYYLIRKKY
                     ITI"
     gene            complement(7522..7722)
                     /locus_tag="KPD58_001750"
     CDS             complement(7522..7722)
                     /locus_tag="KPD58_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265520.1"
                     /translation="MYFKTYILKREVNNKQLEITRSFDLDNLICTATEILEDIEIDLI
                     IIDEKNNVVWRNEKNTPAHENA"
     gene            complement(7741..8154)
                     /locus_tag="KPD58_001751"
     CDS             complement(7741..8154)
                     /locus_tag="KPD58_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265521.1"
                     /translation="MKVKSKRSFIVGIIVCMLCCASLVIYCILKDKRFLISSFLLIVI
                     AIFNFCNAFSRKGIVEELHDSADERDLYLTMKTSHILVKIMNYTLFTFTFLFIIAYSA
                     WKNQSLLVIAITLCVIEIFLFVAYLLINIFLEKKE"
     gene            complement(8138..8371)
                     /locus_tag="KPD58_001752"
     CDS             complement(8138..8371)
                     /locus_tag="KPD58_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0265522.1"
                     /translation="MKTKVREFRTNMGLTQQQLADLVHVSNRTIISIEKEQYSPSLML
                     AYRMAQVFGTTIEELCCLKENKEMEDKKHESKE"
     gene            8590..8964
                     /locus_tag="KPD58_001753"
     CDS             8590..8964
                     /locus_tag="KPD58_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0265523.1"
                     /translation="MKISKLPEAELKVMRYICEANKVLTSRELVAAMEEKYEWNESTT
                     FTVLKRLERREFLSTEKIGKLTHYNILVKEKKYLRFETKEFLKNIHKNSISSLMSALH
                     GEDDEVDEDKLSELEEYFKNLK"
     gene            9576..10268
                     /locus_tag="KPD58_001754"
     CDS             9576..10268
                     /locus_tag="KPD58_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0265524.1"
                     /translation="MYNILVVDDDKEIVDSIEIYLKSEGFKIYKAYDGMKALEIITEN
                     DIHLILMDIMMPKLDGIKATVKIREERNIPIILISAKSEDTDKIIGLNIGADDYITKP
                     FNLLELIARVKSNLRRYVTLGNYEDTCKEILKSGALELNTLTKEVKIDGENIKMTPIE
                     YKIIELLLENKGRVFSIDEIYEKVWNEESFNSENTVAVHIRRIREKIEINPKEPRFLK
                     VVWGVGYKIEKF"
     gene            10464..12524
                     /locus_tag="KPD58_001755"
     CDS             10464..12524
                     /locus_tag="KPD58_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG0265525.1"
                     /translation="MEKYRVLKLVSLIIISISFPLTILLVTIGLAEPKYNIQKTSTKS
                     GIFDISDDFYDSNIFDEGTMASLLPKFKLSVGGKLTSKEKGKFQQEGKSIDLSSSDSE
                     YDKIMINEQASEEYLSYLKNIKVYVINTYTGVFYTNTSYASLYDFKQKTKSYCNVEII
                     NKIGKSSYIKTINGEKFELNNLSLELNEVDEGFEAYVSFPEEPTIEDGIVYTNFQIFK
                     QATEKVRLFFVASLSLAIICLLSIILYIKIKGEVLKRNNVFLLIYSKIPMEFNVILVS
                     ISIAILTIYALAVSYHIDVIALVSLSLLIVSNNIFFINVQLSYLENKMCIFKTSIIVR
                     VILWLIKTVREVSNAIKKVSLARKVIVIVIISLALIMVISTRSIGMGLLFSICIFLGF
                     TIYITKRLSYLSYVIEGTERIKKGELDYKIKITGNDNFTSLAENINNIGEGLDRAIEE
                     QVKSERMKSDLITNVSHDLKTPLTSIINYVDLIKKEESIQPEYINDYINVLESKSKRL
                     KLLIEDLFEASRVSSGNIELQISKIDLVQLLRQSIGELEEKLSKNNLYLKLNVPNDKV
                     YIWADGRRMYRVFENLLSNIAKYSLEETRVYIDVYDYGENVKVTMKNISSYELNFDPS
                     EIMERFKRADESRNTEGSGLGLAIARDLVALQGGKFYIEIDGDLFKANLEFQKYEEEI
                     TNME"
     gene            complement(12730..13053)
                     /locus_tag="KPD58_001756"
     CDS             complement(12730..13053)
                     /locus_tag="KPD58_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBG0265526.1"
                     /translation="MNNNYIFFIIIGMYIVTYLPRVLPMLIFSKKEMPDFLKKIMKFI
                     PVAILTSLTAKDVFFNGDSLYLSIANPKIISFMIVLLVAYKFKSIGISIITGVISIYL
                     FGIIL"
     gene            complement(13046..13747)
                     /locus_tag="KPD58_001757"
     CDS             complement(13046..13747)
                     /locus_tag="KPD58_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBG0265527.1"
                     /translation="MERTQQLKESFIASIPIFLGYIPIGIVGGILLQKSGLSPFQIAL
                     MVTLVFGGSSQFIAASMISTGASVTSIVLTTFIVNLRHFLMSSNLNMYIKNKSPKFIL
                     PFCHTITDETFAVNYEKFTAGNWSDRNAIYLNFFCLISSVLANFIGAFLGESISIDSS
                     ISGFILTSMFIALIVSQIKNKIYIVVFISSIIISVILYALFKSNLVIIVASILASLIG
                     FFIEESYCKKEESYE"
     gene            complement(14025..15410)
                     /locus_tag="KPD58_001758"
     CDS             complement(14025..15410)
                     /locus_tag="KPD58_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG0265528.1"
                     /translation="MPINSFENYPMNWKPKRPSKGQILYKALAEQLEQDINNGFLLPG
                     TKLPPQRELADFLDVNVSTISRAFKICEKKGLISGVTGSGTFVSYDTRSNLFLMSSNN
                     KITFIEMGTMNPDFTLEEMNTLFKHIIKEIGFKTIFQYGQRDGAKWQKEAIAKLIYKA
                     GLETTSDSLLPASGGQNAIVAILAGLFQHGDRIGVDPLTYPGIKTAAKMLGIQLIPIK
                     QEDNEISEEGLLYACKNENIKGLYIIPDYQNPTTHIMSQNGRKMIANIASKYNLIVIE
                     DAIHSLLNETHLNPVASYLPNQTIYITSLSKIIAPSLRLAYISTPKQYREALSSALYN
                     INLSQSYFLTEIAYRMITSGEADKLINARRKSARRRNKIINQYLSGYNLLGNEECIFR
                     WLILPDGIMAEKFEIQALKEGVQVYASERFAVGKEKPISAIRIAVCATESTEELKAGL
                     SVLKRLLEEKK"
     gene            complement(15518..>16418)
                     /locus_tag="KPD58_001759"
     CDS             complement(15518..>16418)
                     /locus_tag="KPD58_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBG0265529.1"
                     /translation="ENFKKEILEIDITSILDEQAKELSINKKLQIYKVKGIYSPAIIG
                     LTRCKIIIPEKKYNNKELRFIFKHELIHYKRKDNLFKLLISIICIIYWFNPILYLLKK
                     YFHELCELSCDEIVIDKCTTSEIKEYAYIILDTIKYQKSLKYSSFVSQFLNKKNIILK
                     KRLNSMFNQDKKKINLGLKFLLTFIIIGSIFSINSNFKQNEDVEYTIGKINYNKNGET
                     YGTHIIDSNGNSIEPELIQTIGRGKKVGYVKKRDLYDCDNQPKTPEEAVLYTRKRNFN
                     IFKKTIPLYDKEGIKIIGRFEIN"
BASE COUNT         6468 a         1786 c         2487 g         5677 t
ORIGIN      
        1 ttacaaagag aattatcaag aaaaacaatc ggtagtttaa atagaaataa agcaagatta
       61 aaagttgcaa gacttcaaga acatatagct aatcaaagaa atgattttct acaaaaacta
      121 tctactaaat taataaaaga gaacgatatt atttgcatag aagatttaca agtaaagaat
      181 atgattagga atcgtaaact atctcgctca atttctgatg tgtcatggtc agagtttatt
      241 cgtcaattag aatacaaggc taattggcat ggtagacaaa ttgtaaaggt gggtaaattc
      301 tttgcaagtt cacaaatatg caataaatgt gggtataaaa acgaggaagt gaaggactta
      361 aatataagag aatggattta tcctagttgt aatgaaactc acgatagaga tataaatgca
      421 agtataaata tattaaaaga aggattaaga ctaataacaa ttcaaaataa ataacgtatg
      481 taagaaccgt aggaactacg gggatagcct gtggagaatt agtaagacat atgttagtat
      541 gcaaaattct gtgaagcagg aaacctgtga ctttagtcat gggaggttca ggctagtatt
      601 actcattatt gtattcaaaa gctaaagtgg aaacctagtg aatatatgaa tttagaagtt
      661 aatgagagag cattagcagc cgactcaata cttattaaaa ttgaagatga agaagaaaca
      721 atgaaagaag ctgaaagaga gaaaaagaga ggtagaagaa ggtagaattt aaagttaaaa
      781 tagttacatt gctaatatat gaaaatgtta caaatttgga aatagatgga tatatatgtt
      841 gtatagtata atgtagataa tatatcacca agtaaaaaac aacaaataaa tctaaattat
      901 gaggggtgga aaataaagat attaaactta caatatgtat atttgctttt gtaattggaa
      961 taatagtgtt attttgctgt ataggtctag gtcaatttaa tatggattgt attataaaac
     1021 aaaatggtgg gtcaatagct accgaacaat acaacatata tttagaacaa tctatttcac
     1081 aatatagaaa ttttggttct atactggttt tactaggagg aataggaata ctgtttgata
     1141 aaagtgtcag aaaatactaa aaaagtatat ttttatttag gatgtgatga atgatatggc
     1201 aagagatgta tttagtgtaa agatgacacc actagaagca cagaaaatgg tagaaaaaaa
     1261 tttggaagca gatttagttt ctgttgatat ttataaccta gaaaataata agtctataat
     1321 agttacaatc tttgaaaaat attatgcaag aagcaatagt gatgcaggtt tggtagtgat
     1381 ttgtgaaaat actacaggaa aaacacttgt taaaattacc tcaactggta gtgcaattgg
     1441 actatttcag attgattggg gaacaggtaa taatttgatt aaaagagtaa aaaaaatact
     1501 tattgattat attatttaat tattttatgt agatattctt aggttggtat ggaatttgta
     1561 ctaacctatt aagtaatcaa taaaactaaa taaacataga aagcacttac aaatatgtag
     1621 gtgcttttta ttgaaaaaat agaaaataaa ataaaaagta agatatagga aaaatatgta
     1681 agaattatat gttataataa ttgtagcaag aagatgtaat ctacaattta tagagtggag
     1741 ttcatgtatt aaaaaattat cctcccaacg ataagaaggg aggtgaatat acatggataa
     1801 ttttttattt aatgtgttag ctagtgtaac agctagttac atagtttatt tgattagcaa
     1861 gttaatcaaa aaagtaaaaa gccactctac cgcaaagagt gacttaaaga ttgatataaa
     1921 ttttaaatat catcgtaaaa aataactttc agttatagaa cttcactcta ccgcaaatag
     1981 attgtagttc ttcttgtttt tattatacca caaattagaa aaaatattgc atataatatt
     2041 ttttatagtc aataaaaata tgaaattttt tatacacaat gatgatgaat ctaactagat
     2101 gaataaattt aattgtaaat aagaaagtac ttacttaaat ggtaggtgct tttgttttgc
     2161 caaattatgc tataatattg agtgagatga gtaatttgtg ggggaatttt ataatgtgga
     2221 tattagtacc aataataaca atagtattac ttataatagc tgtttcttct atgcaatata
     2281 ttttagttat gatagctttt ttgttgatta tatattcttt tatagaaaag aaaatagtta
     2341 tgggtcttgt gtcagttctt tttttcactt attcaattta tctttgtgca acttgggaag
     2401 ataaatcctt gatagcagat aataaagttg agactgttag ggcacaaaga gaaactgtag
     2461 aaagagaaaa agaaatggaa agaagaagaa tacaagaaga agtagacaaa gaaagatata
     2521 ttgaaaaaca tggaatggaa atatcagaaa atgatttaaa agttaagcta gaggctttag
     2581 tacctcaaga atataagggg aagaaatatg aattaaaagt aggaaaattt aaaagatata
     2641 gtatgtattt tgatttaact gtacaaaatg aaaaatttag taattctgaa gaatgtaaaa
     2701 aatttgtaaa agaaattgct aatgatctta aaaaaattaa aataagcaag gcgtacttta
     2761 agtttcattc aaaagatgac ggcggtatat ataattctgt ttatattgat tattttagaa
     2821 acatacaaaa taatgtagat aatgttgaaa atttagaatt caatgagttt gaacttaaaa
     2881 ccgaagaaga agagaagcga gaacaagaga aaatagaaca agaaaaaaac agttataata
     2941 actacattca aaacagagtg gttgacccac tagatagaat aaagaaacta aaagagttgc
     3001 tagattcagg agcaattaca caggaagaat ataataaaaa gaaaaaagaa ttattagaat
     3061 agataatata gtaagcactt acaagtatgt aggtgctttt aaatttacaa agtattccat
     3121 tttaatttta tagtttagat tttatgatat aataaaaata tagaagtttt gcagtgtgcg
     3181 atatttgtta caaagtaggg cttaatactt gaaatctaag atgttgaggg tgcgtgataa
     3241 gtgttatcaa ttgcactatt gcccgctcac tgcaatttta agagtattgt atatatgtag
     3301 gtattggaaa tgctaagttt attttggggt tttagattaa ctatatggaa tgtaaatatt
     3361 acatgggtaa aaattttgat gatttttatg acagttttag attaactata tggaatgtaa
     3421 attgtataga attaagaatt tttttaatac taattatagt tttagattaa ctatatggaa
     3481 tgtaaatatt acatttaatt tgcattgctt tgatgacttc tttgttttag attaactata
     3541 tggaatgtaa atatttttca atatcaatat agaactttca tctttcccgt tttagattaa
     3601 ctatatggaa tgtaaatcaa gatattttta ataaaatttc tgagcgaact gtttgtttta
     3661 gattaactat atggaatgta aatgaggaga ttcagacagc gactaaaaag actgatataa
     3721 agttttagat taactatatg gaatgtaaat aactttatat ctccatttgc atatccatct
     3781 gatgaacgtt ttagattaac tatatggaat gtaaatgcgt acaaggttga gaagctccgc
     3841 aatctcaacg tcgtgtttta gattaactat atggaatgta aatatttgaa aggaataaat
     3901 tttaaatgaa tgaaaggata ggttttagat taactatatg gaatgtaaat cagccaaaaa
     3961 cttcgaattt gaactaaaat gcgtgggttt tagattaact atatggaatg taaattctca
     4021 tgactattga ataaatataa taaacaagca tacgttttag attaactata tggaatgtaa
     4081 atttagaatc aaaagcactt aatcctagtt ctttcatagt tttagattaa ctatatggaa
     4141 tgtaaatagc tttttagctt cgtctactac tttataagca aagcgtttta gattaactat
     4201 atggaatgta aatgatgaac aataaaacaa tcatctaaag acgaagaaag ttttagatta
     4261 actatatgga atgtaaatgt tcctgttgtg ttttttacta atcccatttt atatgtttta
     4321 gattaactat atggaatgta aattgctgtg atttcactaa taaaacaatt aataattgtg
     4381 ttttagatta attatgtgga ttcaaaatta aataaacaaa gaaagcactt acaaatatgt
     4441 aggtgctttt attctgttca aaattggtcg gttgggtaaa ataattagaa aaaattagta
     4501 aaaacctctt ttctgtaact cgttacaata ttattattaa tgtaacgagt tacagaaaag
     4561 aggtgaataa aatagcaact aaaagtaggg cagagtatat gaaaaatcgt cgaaaagata
     4621 aaagaggttt tagtgtactt ttagacaaag aaaagttaga taaatttgat gaagtattag
     4681 aggaaaagaa tctaaccaag aaagaatggc tagaagaaaa aatcgacgag gaactggaac
     4741 aaaaggaata aaaaataagg gtcactccca ccgaccaaag tttgagtaac ccctatgacg
     4801 tatactatca tatatcaatt atagtatatg tcattcctta aaaaaaatca attattaagg
     4861 agtgtaatat tatgaaaaat gaattaatga tgtttgaagg aaaagagatt gaagtatttg
     4921 aatttgaagg aagaatttta ttcaactcaa aagatgtagc aaactgttta gacataaaaa
     4981 atgttaatga gaatataaga cttatgaata aaaagcaagt tgtaaagtta agaaattcgg
     5041 atatcagtaa tactgacatc cgaaaactta ataatgctgg agaaaatttt cttactgaaa
     5101 gtggagtgta taaattaatc tttaaatcaa gaaaagaaga agctgagaga ttccaagact
     5161 ggataagtga tgaagtttta ccaagcataa gacaaactgg tgcatacata actaataatg
     5221 caatcccgaa aagttgagag aaaaagcaag tgagattgaa aaattacagt tgcttataac
     5281 agtacatcta tgttaaaaga attgttagat gatgcaggct ttgacaataa atcaaaacta
     5341 ttaacagcta aaacattata taagaaagca ggaattgatt taccaataga gataaacgaa
     5401 gaagaacatt attttgatac aaaacaaata gcatctaaac tgaaaatata ttctaagagt
     5461 aataaaccag ctcagatggc tgtttgtgag attattaaaa agattgattt agaagatggt
     5521 gaagttaaag gtgtttggta aactaatggt tcttggactg gtactgtaaa taaatataca
     5581 aagagtgtaa tagataaggt gagaacttgg atagaggaaa ataatagacc taccaagatt
     5641 gcaggtgaga agaagaatta tcatgtatct tacaaaattg agtaaattta tcagttgtat
     5701 taaataattt agtttagttt tggggggatt aatacaatgt gtgagaattt acttgataat
     5761 atgtatattg aaaagagaaa agaagaatat agaattaaac ttttgaaaat aagagaaaca
     5821 gatatagata tatataataa gttagaaagt atagtatata aactttctga gaaaaaatta
     5881 gagaaaaata ataaatagac atataaagca cttggatatt ctactgtttc aagtgctttg
     5941 tttggtataa aatggtataa tggagataga gaattatatt aacccaaaaa tggagaaaat
     6001 agagaagatg atttagttga cgctttatca gtaggagtac ttgcaggtaa gacagaatct
     6061 ctagtactat tagttggaaa tggaataagt gatactcaaa aaagtttaat aaataaaaga
     6121 gaatttagaa atataactca aattggtgga aatggaaatg agaaagcatt tagtgaagta
     6181 gaaaacttag ttaaatagat tagaaggtag caactagagt tggttgttac cttctttttt
     6241 tatactttct ctctctaagt attttctttt tcatatcttc taatacatct aatgcagtag
     6301 tataaaattg tatattattt ctataaggta tagctattgg aagaatgccc ttgttaacta
     6361 aaaaaataag aagtacaata aataaaagtg tcataatcat tctaataaaa taattagtga
     6421 tatctgataa atcaaatgtt aagttaattg ttgtagaaaa tacaacagcc ataatagata
     6481 tatacaaagc aaaaatgggg ttactatttt tttgtaactg tcctttaagc ctagattgct
     6541 ctatttctat atcttgaata ctttttatgg aatccatttc actataatac ttataaactt
     6601 ttaaataata ttttttatac ttgtttaaag cttcttcatt atctttaagg tctttgtcac
     6661 ttaactctga tttaacttcc ccaaaatcca taaaatctct cctacaatgt tatatagtat
     6721 actttaattt atagcatatt taagaactta tatcagcaat ttgtcgaaca attttagcta
     6781 aaaatagagt ataatgatag gcttattatt tataagatat tattttttat atagttatat
     6841 atttctttct aattagataa tataatgctg ttgttataat ggctactaat attattgcta
     6901 gtattgtaat atctaatctt cttgtaaata agcctattgc tatatactca agacctgtta
     6961 cataacgata aagtacttct agtttaacag tttttttctt atctatattt ccatatattg
     7021 attctaattt attaaaatat tttttactac aaaaagcagt tttaggttta attaaatata
     7081 ttattgtagt tcctattata actattattc caataagtat aagattgata tttccaaaat
     7141 ccatagcaga tacctcctat taaatttgct tttcttagtc aaagtatatc agtaacacaa
     7201 gtacaaagag tatataaaat attacaaata ttaacaggat agataaaact atagtaaaaa
     7261 ttgtaataat aaagcgaacg aaaattatca tactaaaaca taagacatgt tataattgta
     7321 ttagatagaa gattaacata caccaaagca ctccttataa taaagagtgc ttattttttt
     7381 gaaattcatc aatatataaa ctatcaagaa tcttattcaa cacaccttaa aatcgattta
     7441 aatagctctt tttgatagca taatttatat gatataataa aaaaatagaa gtgtattcgc
     7501 agtacacttc tatagttata actaagcatt ctcatgagcg ggagtgtttt tttcatttct
     7561 ccaaacaaca ttattttttt catcaataat tatcaaatca atttcaatat cttctaaaat
     7621 ttctgttgca gtacaaatta gattatctaa gtcaaaactt cgagttatct ctagttgttt
     7681 gttatttact tctcttttta gtatataagt tttaaaatac atttatatca ccccttgtat
     7741 ttactctttt ttctctaaaa atatattgat aagtaaatat gcaacaaata aaaaaatctc
     7801 aatgacacat agtgttatag ctataactag aagtgattga tttttccatg cactataagc
     7861 tatgatgaaa agaaatgtaa aagtaaataa tgtataattc ataattttaa ctaagatatg
     7921 acttgttttc attgttaagt acaagtctct ttcatctgca ctatcgtgta attcttcaac
     7981 aatgcctttt cttgaaaaag cattacaaaa attaaagatt gctattacaa ttaaaagaaa
     8041 tgatgaaatt aggaatcttt tatcttttaa aatgcaataa ataactaaac tagcacaaca
     8101 taacatacaa actataatac ccactataaa acttcgttta ctctttactt tcatgttttt
     8161 tatcctccat ttctttattt tcttttaaac aacatagttc ttcaatcgtt gtaccaaaca
     8221 cttgtgccat acgataggca agcataagag aagggctata ttgctctttt tcaatagaaa
     8281 taattgttct gtttgataca tggactaaat cagcgagttg ttgctgtgtt agacccatat
     8341 ttgttcgaaa ttctcgtact tttgttttca ttccataacc tctttctata tgaagctaac
     8401 ttcatgtgaa gttaacttca taatatccta aaatgagtaa aatgtcaatc tcattagata
     8461 agtattataa aacctaaaag aataatatgg taaaaattgg atggaattat ttaccttaat
     8521 tctcttgttt gtaaaacttt attgatatag aatttaacat atacataaat cggcaaaggg
     8581 gtgcgaatta tgaaaattag taaattacca gaagcggaat taaaagttat gagatatatt
     8641 tgtgaagcta acaaggtatt aacttctagg gaattagttg ctgcaatgga agaaaaatat
     8701 gagtggaatg aatcaactac atttacagtt ttgaaaagat tagagcgaag ggagttttta
     8761 agtacagaga aaataggcaa acttacacat tataatatac tagtaaaaga gaaaaaatat
     8821 ctaaggtttg aaacaaagga atttttgaag aacatacata aaaattcaat ttcaagttta
     8881 atgtcagcgt tacatggtga agacgacgaa gtggatgaag ataaattaag tgaactcgaa
     8941 gaatatttta agaatttgaa ataagaaaat gacaaattga tacataataa ataaaatgaa
     9001 ggtatgaaat tataactttt tctaccttca ttttgtttat tattctgaac atgttaagaa
     9061 aatgaaataa gtttgtattt tagttatata aattttaata gaatatgtac agtatatagt
     9121 agatagaatg tataaaaata gaatttaaat aagtagctat tattgcagtg aaatgaatgt
     9181 ttctaagtat atttgggtgg aagaatggtg gaagaaagtt agtgaaatgt aattcttcca
     9241 ccattcttcc acccaaaaaa taaaaactgt tcaataagaa ttaatataca tcaattcagc
     9301 tgaatgcaat caaaatactt aaatgaaagt aatctgaaaa caacataatt caaatcaatt
     9361 tattcaaata aacctcaatt tatcttaaat cttaagaaat atttaagaat taggataata
     9421 ttttcttaag atttatattt tattatatag ttataaggga aagttacaaa aatgtaagcc
     9481 aaagtaatat aacaatttag tcattgttga gaaagggagt gaaagatatt gtataatata
     9541 gataagtatt attatacaat agaggaagtg aaaatatgta caatatatta gtagtagacg
     9601 atgataaaga aattgttgat tcgatagaaa tatatcttaa atcagaagga tttaaaatat
     9661 ataaagcata tgatggaatg aaagctttgg aaataataac agagaatgac attcatctaa
     9721 tactaatgga tataatgatg cctaaattag atggaatcaa agcaactgta aaaatacgag
     9781 aagaaagaaa tattccaata attttaattt cagcaaaaag tgaagataca gataaaataa
     9841 taggattaaa tattggagct gatgattata taacaaagcc atttaatcta ttagagctta
     9901 tagctagggt taaatcaaac ttaagacgat atgtaacttt aggtaattat gaagatacat
     9961 gtaaagaaat tttaaaaagt ggagctttgg agttaaatac actaacaaaa gaagttaaaa
    10021 ttgatggtga aaacataaaa atgactccaa ttgagtataa aataattgaa cttcttttag
    10081 aaaataaagg acgagttttc tcaatagatg agatatacga aaaagtatgg aatgaggaaa
    10141 gttttaattc agaaaataca gtagcagtac atatcagacg aataagagaa aagattgaaa
    10201 taaatccaaa agaaccaaga tttttaaagg tggtgtgggg agttggttat aagattgaaa
    10261 aattttagtg gaaaaattat aaaagaaaag agtttaatat ttaattgcat tttgatttta
    10321 gtcattttta catttacaca aatgttagga attttaatat gttttaattt tttctctgag
    10381 agtttaattt actctatggg gattatgctt ttcttaaact ttattttata taaaatactg
    10441 acaatgtaga tgaggtgggc aacttggaga aatatagagt attaaagtta gtttcattaa
    10501 tcataatatc tatctcattt ccattaacta tattgttggt gacaatagga cttgctgagc
    10561 ctaaatacaa tatacaaaaa acttctacaa agagtgggat ttttgatata tctgatgatt
    10621 tttatgatag taatatattt gacgaaggaa caatggcaag tctattaccc aagtttaagc
    10681 tatctgtagg tggtaaatta actagtaaag aaaaaggtaa attccaacaa gaaggtaaaa
    10741 gtatagattt aagtagtagt gattctgagt atgataaaat catgattaat gaacaagctt
    10801 ctgaagaata tttaagttat ctaaaaaata taaaagtgta tgttattaat acatacacag
    10861 gagtatttta tacaaatacg tcatatgcta gtttatatga ttttaaacaa aaaactaaga
    10921 gctattgtaa tgtagagata ataaataaga taggaaagag ctcatatatt aaaacaataa
    10981 atggagaaaa atttgaactg aataatctga gtctggaact aaatgaagta gatgaaggtt
    11041 ttgaggcata tgtatctttt ccagaagagc caactataga agatggaata gtatatacta
    11101 attttcaaat atttaaacaa gctacagaaa aagtaagatt attttttgta gctagtttaa
    11161 gtttagcaat tatatgttta ttatctataa ttttatatat caaaataaaa ggtgaagttt
    11221 taaaaagaaa taatgtattt ttactaatat acagtaagat acctatggaa tttaatgtaa
    11281 tattagtatc tatttcaatt gcaattttaa ctatatatgc tcttgctgta tcttatcata
    11341 ttgatgtaat tgcattagtt tctttatctt tactgatagt atcaaataat atatttttta
    11401 taaatgtgca attgagttat ttagaaaata aaatgtgtat atttaagaca agtattatag
    11461 taagagtgat tttatggtta ataaaaactg taagggaggt cagtaacgct ataaagaaag
    11521 tatctttagc aaggaaagtt attgttatag tgataatatc tttagcttta atcatggtaa
    11581 ttagcactcg aagtataggt atgggattac tgttttctat atgtattttt ttaggattta
    11641 caatatatat aactaaaaga ctttcatatc ttagttacgt aatagaaggt acagaaagaa
    11701 taaaaaaggg agaattagat tataagataa aaattactgg gaatgataat tttacgtctt
    11761 tagctgaaaa tataaataat attggagaag gtcttgatag agctatagaa gagcaagtaa
    11821 aaagtgaaag aatgaagtct gaccttataa caaatgtatc tcatgatttg aaaactccac
    11881 ttacttctat aataaattat gtagatttaa ttaaaaaaga agaatcaata caaccagagt
    11941 atataaatga ttatataaat gtacttgagt caaagtcaaa aagactaaaa ttattgatag
    12001 aagatttatt tgaagcgagt agagtaagta gtggcaatat agaattacaa attagcaaga
    12061 ttgatttagt gcaactatta agacaaagta taggtgaact tgaagaaaaa ttatctaaaa
    12121 ataatttata tttaaaatta aatgttccaa atgataaggt gtatatatgg gcagatggaa
    12181 gaagaatgta tagagtattt gaaaatcttt tatctaatat agcaaaatat tccttagaag
    12241 aaactagagt ctatattgat gtatatgact atggtgaaaa tgtaaaggta actatgaaaa
    12301 atatatcttc ttatgaatta aattttgacc caagtgagat tatggaaaga tttaaaagag
    12361 ctgatgaatc aaggaatact gaaggaagtg gattagggct tgctattgca agagatttag
    12421 tagctttaca aggtggaaaa ttttatatag aaatagatgg agatttattt aaagcaaatt
    12481 tagagtttca aaaatatgaa gaagaaataa caaatatgga atgagatagt atttaagtat
    12541 atatattttt tttactgtcg aaagctttta ttataatata ttagaatatt tacagtagag
    12601 aatgaagagt aaaaacagag taactttaag tcactctgtt ttttatttaa aatcaataat
    12661 ataacaactt aaattaaaat tgtacacata gttttaaaat aacttataca ataaaacatt
    12721 atccaaatat cacaagataa ttccaaataa atatatagat atcactcctg taataataga
    12781 aataccaata gatttaaact tatatgcaac aagaagtaca atcataaaag atataatttt
    12841 agggttagct atagataaat atagactatc tccattaaaa aatacatctt tagcagttaa
    12901 ggaagtaagt attgctacag gtataaattt catgattttt ttcaaaaaat caggcatctc
    12961 tttttttgaa aaaattaaca taggaagcac tcttggtaga tatgtaacaa tgtacattcc
    13021 aattataata aaaaatatat aattattatt cataagattc ctccttttta cagtaagatt
    13081 cttctataaa gaaaccaatt aaagatgcta aaattgatgc aactattatt actaaattgc
    13141 ttttgaataa tgcatataaa ataacagaaa taatgattga tgatataaat actacaatat
    13201 aaatcttatt ttttatttga gaaactatta aagctataaa catagaagtc aatatgaagc
    13261 cagaaataga actgtctata gatatacttt ctcctaaaaa tgctccaata aaattagcta
    13321 aaactgatga aattaagcag aagaaattaa gataaatagc atttctatca gaccaatttc
    13381 cagcagtaaa tttttcataa ttaactgcaa aagtttcatc tgttatagta tgacaaaatg
    13441 gcaatataaa ttttggactt ttatttttta tgtacatgtt caaattagaa ctcattagaa
    13501 aatgtcttaa gttaactata aatgtagtca aaacaataga tgttacactt gcaccagtag
    13561 aaatcattga agctgcaatg aattgagaac ttcctccaaa tactaaagtt accattagtg
    13621 ctatttgaaa aggtgaaaga ccagattttt gtaatagtat tcctcctact atacctatag
    13681 gtatataacc taagaatata ggaattgaag caataaagct ttcttttaat tgttgagttc
    13741 tttccacaat aaatcactcc ttttgtatta aatgtattaa agtattcgta aaacagaaaa
    13801 atagattaat tgtataattg caattataat taaaaataaa tttggattaa tatataaaat
    13861 actatacaac aaatagataa tcttaaacgg ttattaaatt ttatctattt gttattatga
    13921 tgtaataata catattaatc tctataagtt aattactatc tggtgtaatt ttattttaac
    13981 aacaaaaaaa aatacacaca attaaaatat tgtgtgcaat acaattattt tttctcttct
    14041 aatagtcttt ttaatacgct caaaccagct ttaagttctt ctgtactttc tgtagcacat
    14101 acagctattc ttattgcaga aattggtttt tcttttccaa cagcaaaacg ttcagaagca
    14161 tagacctgta caccttcttt aagtgcttgt atctcaaatt tttctgccat tattccatca
    14221 ggtagaatca accatctaaa tatacattct tcatttccca aaagattata tccagataaa
    14281 tattgattaa taatcttgtt tcttcttctt gcagatttac ggcgtgcatt aattaattta
    14341 tcggcttcac ctgatgtaat catgcgataa gcaatttctg ttaaaaaata agattgagat
    14401 agattaatgt tgtataatgc gcttgataat gcctctcgat actgtttagg tgtagagata
    14461 tatgctagcc gtaaactagg tgcaataatt tttgataaac tcgtaatata aattgtttga
    14521 ttgggcagat aggaagccac tggatttaaa tgtgtttcat taagtaaact atgaatagca
    14581 tcctcaataa caattaaatt gtatttagat gcaatatttg caatcatttt tcgtccattt
    14641 tgtgacatta tatgtgtagt tggattttga taatctggta ttatgtatag acctttaata
    14701 ttttcatttt tacaagcata taatagtcct tcttcactaa tctcattatc ttcttgttta
    14761 attggaatta gttgaatccc aagcatttta gctgctgtct ttattccagg ataagttaat
    14821 gggtctacac ctattctatc tccatgttga aacaaaccag ctaaaatagc aacaattgca
    14881 ttttgtccac cacttgcagg aagtaagcta tctgatgtag tttcaagccc agctttataa
    14941 attaatttgg caatagcttc tttttgccat ttagcaccat ctctttgtcc atattggaat
    15001 atggttttaa aacctatttc cttaattatg tgtttaaaca aagtattcat ttcttctagc
    15061 gtgaaatctg gattcattgt gcccatttca ataaatgtaa ttttattatt actggacatc
    15121 aagaatagat ttgaacgtgt atcataagat acaaaagttc cacttcctgt aactccactt
    15181 attagacctt ttttttcaca aattttaaaa gctctagaga tagtactcac attaacatct
    15241 aagaaatctg ccaattcacg ttggggtggt aatttagtac ctggtaataa aaatccattg
    15301 ttgatatctt gttctagttg ttctgcaagc gccttatata atatttgccc ttttgaaggt
    15361 ctctttggtt tccagttcat aggataattt tcaaaagaat taattggcat aagttttcac
    15421 ctcacattat taatttgatt ttttctttat ctaaaacaat aaattctaaa gatacaaata
    15481 aaaactgata ggaaataatt ctatcagttt ttgtctttta attgatttca aatcttccta
    15541 taatttttat accttcttta tcataaagag ggattgtttt tttaaatata ttaaaatttc
    15601 tttttcttgt atataaaaca gcttcttctg gtgtctttgg ttgattatca caatcgtaca
    15661 aatctctttt ttttacgtag ccgacttttt tacctctacc tattgtttgt ataagttctg
    15721 gttcaatgga atttccattt gagtctatta tatgtgtacc ataggtttct ccatttttat
    15781 tataattaat cttaccaata gtatattcta catcttcatt ttgtttaaaa ttggagttga
    15841 tagaaaatat tgaccctatt attataaagg ttaataaaaa cttaagacct aaattaattt
    15901 tctttttatc ttgattaaac atagaattta acctcttttt taaaataata ttttttttat
    15961 ttagaaactg agatacgaat gatgaatact tcaaagattt ttgatacttt attgtatcta
    16021 atattatata tgcatactct ttaatttcac tcgtggtaca cttgtcaatc acaatttcat
    16081 cacaagataa ttcacataat tcatgaaaat atttttttaa taagtataaa attggattga
    16141 accaatatat gatacaaatt atactaatta gaagcttaaa taagttatct tttcttttat
    16201 aatgaattaa ttcatgttta aagatgaatc ttaactcttt attattgtac tttttttctg
    16261 gaattattat tttacagcgt gttaaaccta taatggcagg agaataaata cctttaacct
    16321 tatatatctg aagtttttta tttatactta gttcttttgc ttgttcatct aagatactag
    16381 taatatctat ttctaaaatt tcttttttaa aattttct
//
LOCUS       DAELLV010000068        16304 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.068, whole genome shotgun sequence.
ACCESSION   DAELLV010000068 DAELLV010000000
VERSION     DAELLV010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16304
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.068"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..674)
                     /locus_tag="KPD58_001760"
     CDS             complement(<1..674)
                     /locus_tag="KPD58_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG0265530.1"
                     /translation="MIIEKILNNNVIITTDENHKEIVVMGRGLAYKKRTGEHISKDKI
                     DKIFKLSDPNISDKFKELIADIPIRYMELSDEIILYAKEKLGKRLNDSIYISLTDHMY
                     TAIERAREGVSVKNVLLWDIKRFYKSEFKIGLEALDYIEKKFEIRLSEDEAGFIALHI
                     VNAQMDQSIKTIYEITQIIQEISNMVKYHYRIVFDEDSVYYHRFITHLKYFAERIVSN
                     NLHENN"
     gene            complement(1025..2212)
                     /locus_tag="KPD58_001761"
     CDS             complement(1025..2212)
                     /locus_tag="KPD58_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG0265531.1"
                     /translation="MIESIKKRAYEIENGLGKEIEEVCNFIFNNPELGEEEYISSKYL
                     VEKMKEYGFDTVYPYCNMETAFRAELGDNDGPTIAFLAEYDALPGYGENKEPAHACGH
                     NWIAASTLGACIVLSKLKENFKGKIVLIGTPAEETTGGKCDLVKAGAFDDVDVSYQMH
                     IEAFNNINCKALAIDSLEFSFEGVSAHAASHPHMGVNALDAVQLTFAGINALRQHVKS
                     DVRIHGIVSNGGEAPNIVPEKAACKFFVRAAERSYLDEVTKKVINCAKGAELMTGAKL
                     SYRYFENSFDNIINNKVLQKITKNNLIEAGITDILEGKDGPVGSTDIGNVSQVCPTMY
                     TEIALDISPMVYVHEKEFLNYANSEEAYDKLHKAVKAMVGCALEIYLEDGLLDEIKKN
                     HLN"
     gene            complement(2226..3617)
                     /locus_tag="KPD58_001762"
     CDS             complement(2226..3617)
                     /locus_tag="KPD58_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBG0265532.1"
                     /translation="MSSLKKKKFTMPQTLTIIFLLIVLMAILTWIVPSGNFERVDIDG
                     RSVVVAGTYEKAPSNPQGITDVFTAPINGFIDAAEVVGFVLIVGGAFGIVNKTGAIEA
                     VIAHTVNKMKKFQFLIIPISMILFGLGGTTFGMSEETLPFYMIFIPLMTSMGYDSLTA
                     VATVFIGATAGFGAATTNPFSVGIAQALSQIVPGSGIEFRVVMFIIYMAISIGFVMMY
                     ANKVKKDPKKSLVHDISLNQELMVNSDTNIKEFTKREAMVIAIFTIGMAIMIYGVLRL
                     EWYITEIAMIFTAIGIISGIASGLKQDEIVNSFISGAGDLITAGLCIAFARGIVIIAE
                     NGFIIDTILNSAANLLNGLPKTIFINLTFLIEGLIAFLIPSASGLASLTIPVLAPLGD
                     LVDVSRQMIVTAYQFGIGVTNLITPTSGVLMGALAVANIPWSKWVRFVIPLMVVLTIL
                     VMAFLTIGLYLGF"
     gene            complement(4212..5912)
                     /locus_tag="KPD58_001763"
     CDS             complement(4212..5912)
                     /locus_tag="KPD58_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG0265533.1"
                     /translation="MHYIAWHFNKNGGNNTMKDKLQVLSGAMMIPVILLVVAGVFIGL
                     GSAFANMENVSALGLSGLIKEGSFINVFFKVINDLGFMVMRYLPIFFVTGIAFGLSKK
                     EKGWGALGGIVLFIGMHTVISTLLAANGINSDTVTAEAFMASGLGEAEAYSRSSLYGS
                     FLGIFSYDCSIFGAIIAGFVASAVHNKFVDIELPSALSFFSGPRFSMIMMFIASIVLG
                     TAMYFIWPPVGLALSKLGNWIGSSGLLGTFTFGAMDKALLPFGIHHLIAFPIEYTRVG
                     GTMEVGGAIYEGVNNIRLAQMGDPDTLSYITRNFTTGRLLIHFAILPAAALAMYKLAD
                     NANKKKAISILIPAIVTAMLVGVTEPIEYTFLFVAPLLYFVAYVPLAGLTYVFTEMFN
                     VSIMGESFRNMFPNLLQPQKVDALPLLFLIPIFFIVTYIIFTWAIKKFDIKTPGRSSE
                     EVKLYSKKEYRERQSVDAGKTAYNNAVEEEVDDKDTKLVHSIIEGLGGSNNIKNVTNC
                     ATRLRVELNSIDGFYEDGFWVNELGASGVVKKKNSVQVIFGPRVITIASKLKAVLGVD
                     "
     gene            complement(5884..7287)
                     /locus_tag="KPD58_001764"
     CDS             complement(5884..7287)
                     /locus_tag="KPD58_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG0265534.1"
                     /translation="MIVNKELIIDYLKSNQENIINDIRSLVEIPSIRDESTTDINQPF
                     GIEIRNAFDKLIQIAKDKDFVVKDFDGYAIHIEYGEGEEVVGVLNHIDVVPIYNKELW
                     KSKPFKVCQKDNYLYGRGVNDNKGPLIGILYALLFLRELNEKPKRKIRLIVGGAEETT
                     WECMEHYFSVNEQPKFAFSPDGNFPIVNGEKGILYFNLRKKIDKDKFRNHNLVDIKSN
                     KEDGFVCNKIEAVFKTNDKKDLVESLAYYTEIEELEEGKVLVRYTGERALSRNPHRSY
                     NCAFSLAKDLEKIKKLNDKGVIIRDILNSYFTDDNHGKKLGIYKEDVDMGVSTICIMS
                     IFLEKNELNMKIDFRYPKGISWEFITNRINEIGKKENLIVDIYKDLKLLYVKPDSELI
                     NKLSNAYKQGFGKEAELFTKGAASYARVLKNGVAFGPTIEGDNPNSHQANENISIDTL
                     YKAIEVYIYALYSLAFQ"
     gene            complement(7551..8042)
                     /locus_tag="KPD58_001765"
     CDS             complement(7551..8042)
                     /locus_tag="KPD58_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG0265535.1"
                     /translation="MFNIFKKKENKIEKIYSPFKGNLVNLEDVPDAVFAQKMMGDGVA
                     IIPSEGKLYSPVDGEIVNVFDTKHAIMIKSNDNTNILIHVGLETMSLEGKPFDVKVKA
                     GDKVKAKDLIMEVDLDFITSNDLNIITPVVIMDDADSVKRTVKDRIEPGNVDINSIIM
                     EVE"
     gene            complement(8068..8880)
                     /locus_tag="KPD58_001766"
     CDS             complement(8068..8880)
                     /locus_tag="KPD58_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG0265536.1"
                     /translation="MRYKLICTDMDGTLMGKGFEVSEENIKALKEVMEKGIKVALVTG
                     RPYNAMKYFTSVLGDDIYIISTNGTYFKLLGYEYKKVLSKETLKKIYTIGEKYNLNKH
                     FKGCKIVISNNEIGEEHPYRLINSKNKEEDRIEIIENASCETLLEKADNEILKCILFS
                     ENVDSLREAKEEFKKQEDLEVVSSGKINFEVMSKGTSKGIAVKKFCDILGIDSEEVIC
                     IGDNENDISMIKFAGLGIAMGNATDEVKSMADFVTDTNVNDGVAKALRKILS"
     gene            complement(8893..10044)
                     /locus_tag="KPD58_001767"
     CDS             complement(8893..10044)
                     /locus_tag="KPD58_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBG0265537.1"
                     /translation="MINFYQPSRINFGQGTLSELPRIIGKYGKKCLLVTTPNVEPLDK
                     LYKRVTLNLIEKGIEVVHFDRVEPNPTVELINEGFELAKKESVDVVLAVGGGSSIDTA
                     KVLALTFGLEKIDWDYMFSTYTNPFKIYDKVSEKELPLIAVSTTSGTGSQVTQAAVIS
                     RGSEKNTIFHQNCFSKECIVDPELMLTLPERITASTAFDAFTHAFESYINPNANDFTE
                     IMSEKSMELVINYLPKVMEDKNNIEYRTKLAVADTLGGSSLANAGAAAPHPLSEIIGG
                     VTHIPHGEALAIVFPKFAEKFYKDNIEKFAKVARMLNQELEKVEDDEAAKTLGNEIES
                     FLKRVGLKTKMSEFNVTKEQLEQITSCPVLGFLPFGKKEDLVEILEKSY"
     gene            10314..10955
                     /locus_tag="KPD58_001768"
     CDS             10314..10955
                     /locus_tag="KPD58_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HBG0265538.1"
                     /translation="MKYLIDSANLDEIQKCLKYGFKGITANPSMYLKEKVNFHEFIET
                     CANLNPEILTAEVIGNNLYELINSCDKILNINKNTIIKINFSEVGLELINILNSKGVK
                     TACTLIFNVNQASLAINAGADYLFPFIGRNDENGNDGIKNLTEICNLISINGYNTKVV
                     AASIKNVFHLTQASLCGCDYAAVTYDLFKKASFNQLTIDGANTFERDWSKVNK"
     gene            10981..12525
                     /locus_tag="KPD58_001769"
     CDS             10981..12525
                     /locus_tag="KPD58_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG0265539.1"
                     /translation="MNKRLLDIAELLLNSSDYITVDTIAEELKVSNKTIRNDLVILDE
                     WLLEFNLSLDKKTGSGVIILGNEDIKLKVIRDINDKSNCIYAYSPEDRKRYILSKLFI
                     KNSKFRIRDICNELHVSRATVHKDLVVIQNFLQNFKVTLVRKTNNGVYLEGKEKDIRK
                     AIFELATVNKSYTELKEILFSKSNECKDTPSTKIFKELFNYDFEKLSQITLHTLQVEK
                     SNLSDEYYINFLIHIAICIKRISIKRYINLSEGFFRELLEHEYFEKSKILCANLSEAF
                     DVEFIDEEVCYILLHIEGLLKSIKKTTTLEPLKNEEITEYNLSEAIAVHWGSILNLNL
                     KDDLILIKSLANHLKSVLHRINYGFTITNPILNDIKRTFPYTYKAAKESNTVIKQLMN
                     YEVNDDEIGYLALYLISAIDRSKSPLNTILICHCSDGVSNLLVQKLSFEFNQINIVKS
                     IPLSSISFTNFDDVDLILTTAPVDFEHNAELININALLSKNDISRLSTIIKKLYSEKN
                     KILSYK"
     gene            complement(12725..13351)
                     /locus_tag="KPD58_001770"
     CDS             complement(12725..13351)
                     /locus_tag="KPD58_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG0265540.1"
                     /translation="MKKEMIKDYLLITISAFLMALAVNFFFAEHTLAPGGITGLSVVL
                     STFLSMPVENISLGISMPLLVMGLLFLGKGFGIKTLYITLMGPLFLKVIPQTHITDNL
                     LVAGVIGGLLVGTAIGIAIIRGCSTGGTDLVAMLINKVIKFLKLPVILFMLDGFIIVA
                     SGIISKNFMISIYSLISLLLIIKTIGFVTTKFGKNTEEINNINNACAQ"
     gene            complement(13486..14202)
                     /locus_tag="KPD58_001771"
     CDS             complement(13486..14202)
                     /locus_tag="KPD58_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentose-5-phosphate 3-epimerase"
                     /protein_id="HBG0265541.1"
                     /translation="MKHIKIAAGLAHVNYGHISAVVKEAMDAGVDYVHSDAADMHDLK
                     NMQLMGGHQIIEAIRPVTDKPIECHIYTRDCDRLFIEKIAAAGCNLLIIPAEHFLGAP
                     LAYIINYCREFGMKVGLTIGCYTPLCFVDEAIYDIDRLQIVVHGVDETDGKDNWGWRK
                     SAVDLVKRARKMIDEKNPKCELAIDGGLRSDNMEPLIECNPDVVILSSAIFKDKDGIT
                     AGVKNCRKAIDEAATKFGLE"
     gene            14628..15842
                     /locus_tag="KPD58_001772"
     CDS             14628..15842
                     /locus_tag="KPD58_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBG0265542.1"
                     /translation="MRNCIIAQSGGPTAVINASAVGIYEKNLETKYFDKVYFGINGIE
                     GILNKNIIDSDSLNKDNVLNLKQTPSSGLGSCRYKLADYKINSYDYDRVINILDEYKI
                     DTLFYIGGNDSMDTVMKFSQYCKNTTCKIKFIGIPKTIDNDLLGTDNCPGFGSAAKLI
                     STLIMETYLDFSIYKNNGIFIVETMGRDTGWLAASSSLARLNDTPLVDLIYLPEIDFY
                     EDKFIEDVRKIFKEKNKAYIVASEGIRDKYGNFICEQKTSAHDKFGHSQLGGVSNYLK
                     NLIVSSSITTRVKTLELGVIQRCAMHLASDTDLKQAAEVGAYGIELSKKGYTGVMAII
                     NRTCNNPYKYEVSHTDISKIANKTKYFPKEWINEECNYITNEALNYLYPLIQGKPDIK
                     YKNGLPYFSIIY"
BASE COUNT         5412 a         2789 c         1860 g         6243 t
ORIGIN      
        1 tcattatttt catgtaaatt attactaaca attcgttctg caaaatactt taagtgagta
       61 ataaatctat gataataaac tgaatcttca tcaaatacta ttctgtaatg gtatttaacc
      121 atatttgaaa tttcttgtat tatttgtgta atctcatata tagtttttat actttgatcc
      181 atttgtgcat taactatatg cagtgctata aatccagctt catcttctga tagtctaatt
      241 tcaaattttt tttctatata atccaatgcc tctaacccta ttttaaattc actcttataa
      301 aatcttttta tatcccaaag taatacattt tttacactta ctccttcacg agctctttct
      361 attgcagtat acatatggtc tgttaaagaa atataaatac tatcatttaa tcttttccct
      421 agtttttctt tagcatataa aattatttca tctgataatt ccatatatct tataggaata
      481 tctgctatta gttctttaaa tttatcagat atatttgggt ctgaaagttt gaatatctta
      541 tcaattttat cctttgatat atgctctcct gtacgttttt tataagctaa acctctcccc
      601 ataactacta tttccttatg attttcatca gttgtaataa ttacattatt attgagtatt
      661 ttttcaataa tcattgtgat gcccccttta ttagcattaa gttaaaataa atctttttta
      721 attatttaga atatttaaaa tgtaatttat tacataaaaa aaaacctatt tattacacat
      781 taaatagact cccatgagtc ctgagtaata aataggttta gcttgtattt aaacaatcac
      841 tatccgtttg tattttattt taattattat ttattatatg ttagcaaatt tattttttag
      901 tgtcaacttt ttctcaatat tttaatttta aataaattat ataaattgaa tcttcaaagt
      961 aatattttta tcaataaaca aaataaaaaa ggtattactt tctttaagta atacccattt
     1021 atttttaatt taagtggttt ttcttaatct catcaagtag tccatcttct aaataaattt
     1081 ctaatgcaca tcctaccatt gcctttactg ctttgtgaag tttatcataa gcttcctcag
     1141 aatttgcata atttagaaat tctttttcat gaacatatac cattggactt atatctaagg
     1201 ctatttctgt atacatagta gggcaaactt gacttacatt acctatatct gtagaaccta
     1261 ctggaccatc tttaccttct aatatatctg ttatacctgc ctcaatcaaa ttattctttg
     1321 ttatcttttg aagtactttg ttatttatta tgttgtcaaa agaattttca aagtatctat
     1381 aagaaagctt agctcctgtc attaactctg caccttttgc acagtttata acttttttag
     1441 ttacttcatc taaataactt ctttcagcag ctcttacgaa aaacttacag gctgcttttt
     1501 ctggaactat atttggagcc tctcctccat ttgaaactat accatgtatt ctaacatctg
     1561 attttacatg ttgtcttagt gcatttatcc cagcaaaagt aagttgtact gcatctagtg
     1621 cattaactcc catatgaggg tgactagctg catgtgctga taccccttca aaactaaact
     1681 ctaatgaatc tatcgccaaa gccttacagt ttatattatt aaaagcttct atatgcattt
     1741 gataacttac atcaacatca tcaaaagctc ctgccttaac taaatcacat tttcctcctg
     1801 ttgtttcttc agctggtgtt cctattagta caatttttcc tttaaaattt tcttttaact
     1861 tacttaatac tatacaagct cctagtgtag atgctgcaat ccagttatgt ccacaagcat
     1921 gagctggttc tttattttct ccatatccag gaagtgcatc atactcagct aaaaatgcta
     1981 tggtaggtcc atcattatct cctaattcag ctctaaaagc agtctccata ttacaatatg
     2041 gataaactgt atcaaatcca tattccttca ttttttctac taagtatttt gatgatatgt
     2101 attcttcttc ccctaattca ggattgttaa atataaaatt acaaacttct tcaatttctt
     2161 ttccaagtcc attctctatc tcataagctc tcttctttat actctctatc ataatttacc
     2221 tcctactaga atcctagata taatcctatt gtcaaaaatg ccattacaag aattgttaat
     2281 actaccatta aaggaattac gaatctaacc cactttgacc aaggaatatt ggctactgct
     2341 agtgctccca ttaaaacacc tgatgtaggt gttatcaagt tagttacacc tataccaaat
     2401 tgataagctg taactatcat ttgtcttgat acatctacta agtcgccaag tggtgcaagt
     2461 acaggtattg taagtgatgc aagccctgat gctgatggta ttaaaaatgc tattaatcct
     2521 tctattaaga aagttaaatt tataaatata gtttttggta atccatttaa taaatttgct
     2581 gcagaattta aaattgtatc tattataaat ccattttctg ctattattac gattccacgt
     2641 gcaaatgcta tacacaatcc tgctgtaatt aaatctcctg ctccacttat aaatgaatta
     2701 actatctcat cttgtttaag ccctgatgct attcctgata ttatacctat agctgtaaat
     2761 atcattgcaa tttcagttat gtaccattct agtcttaaaa ctccatatat cataatcgcc
     2821 atacctattg tgaaaatggc tattaccatg gcttctcttt ttgtaaattc ctttatattt
     2881 gtatcactat taaccattaa ttcttgattt agtgatatat catgtactaa agatttttta
     2941 gggtcttttt taactttatt ggcatacatc ataacaaatc ctatagatat agccatatat
     3001 attataaaca taacaactct aaactctatt ccagaacctg gtactatttg tgataatgct
     3061 tgtgctattc ctacagagaa aggattcgta gtagctgctc caaatccagc tgttgctcct
     3121 ataaatactg tggccactgc tgtcaatgaa tcatatccca tactggtcat aagtggaata
     3181 aatatcatat aaaaaggtag tgtctcttca ctcattccaa acgtcgtccc acctagtcca
     3241 aatagaatca ttgaaatcgg tattattaaa aattgaaact tcttcatttt atttacagta
     3301 tgagctataa cagcttctat agctcctgtt ttattaacta tcccaaaagc tcctccaact
     3361 attaaaacaa atccaactac ttcagctgca tctataaacc cgtttatagg tgctgtaaat
     3421 acatcagtaa ttccttgtgg atttgatggt gctttttcat aagttccagc tacaacaact
     3481 gaccttccgt ctatatctac tctttcaaaa tttccacttg gcactatcca agtaagaatt
     3541 gccattaaca ctattagcaa aaatattatt gtaagtgtct gtggcatagt aaactttttc
     3601 ttttttaaac tgctcatttt taatctcccc actttttaaa ttaaatattt tattattgca
     3661 tccctttatc ctattttgta tagatgtaaa aaagctcaac atggatacat gttgagctaa
     3721 atgtgctaaa tacaacatac ctgcatcttc cctttatctt gtttaagata gctctccatc
     3781 aaaattcggg ataattttga tgacagtact gtacctatta aatacagtcc cagcactagc
     3841 ttcggacact agcacttcgg cacttactcc ttttatattg cctcattggt ttccaacctc
     3901 ttcaatatta atcttagtaa atgcaacctc tacctcaatc tgtttgttag atgaggtaat
     3961 tcttattaat ttttattaat tcaaagtaat tgtagcataa tatgtcgaga ttgtaaatat
     4021 tttcagtata ataattctta ctttatgatt tcattccatt tttcacaaat aattttttct
     4081 ccatataatt taaatactgt agtatatttt aaatacacac aaactttcca caaattttaa
     4141 attattataa tacctttata ataaaaaata aaagctatgt aaacttatca atttacatag
     4201 ctttatatac attaatctac tccaagtaca gctttaagct ttgatgctat tgtaataact
     4261 cgtggaccaa atataacttg aactgagttt ttctttttaa caactccact tgcacccaat
     4321 tcatttaccc agaagccatc ttcatagaat ccatcaattg aatttagctc taccctaagt
     4381 ctagttgcac agtttgtaac attttttata ttattacttc ctcctaaacc ttctattata
     4441 gaatgtacaa gctttgtatc tttatcatct acttcctctt ctactgcatt gttgtatgct
     4501 gttttaccag catctacaga ttgtctttct ctatattctt tcttactata aagcttaact
     4561 tcttctgagc ttcttcctgg tgtttttata tcaaacttct tgattgccca tgtaaatatt
     4621 atataagtaa ctataaaaaa tattggtatc aaaaataata atggtaacgc atccactttt
     4681 tgtggttgta ataaattagg aaacatattt ctaaatgatt ctcccattat agaaacatta
     4741 aacatttctg taaatacata cgtcaatcct gccagtggta catatgctac aaaatataat
     4801 aatggtgcaa caaataggaa tgtatactct attggttccg ttacccctac caacatggct
     4861 gttactattg ctggtatcaa tatacttatt gcttttttct tattagcatt atcagccaac
     4921 ttatacatag ctaaagctgc tgctggtaat attgcaaaat gtattaataa acgacctgtt
     4981 gtgaagttac gagttatata agatagtgta tctgggtctc ccatttgagc taatcttata
     5041 ttattaacac cctcgtaaat tgctccacct acttccattg ttccacctac tcttgtatat
     5101 tctataggga atgcaattaa gtgatgtata ccaaatggta ataatgcttt atccattgct
     5161 ccaaaagtaa atgttcctaa taatccacta gaacctatcc aattaccaag tttagataat
     5221 gccaacccta ctggtggcca tataaagtac attgctgtac ctaatactat tgatgctata
     5281 aacatcataa tcattgaaaa tcttggacct gagaaaaaag ataatgcact aggaagttct
     5341 atgtcaacaa atttgttatg tactgcacta gctacaaatc cagctattat agctccaaat
     5401 atactacaat catatgaaaa gattcctaag aaagaaccat ataaagaact tcgactatat
     5461 gcttctgcct ctcctaatcc agatgccata aacgcttctg ctgtaactgt atctgaattt
     5521 ataccatttg ctgctaacaa tgtacttata actgtgtgca ttcctataaa taaaacaatt
     5581 cctcctaatg caccccatcc tttttccttt ttagataatc caaaggctat acctgtaaca
     5641 aagaatattg gtaagtatct cattaccata aatcctaaat cgtttattac tttaaagaat
     5701 acatttataa atgacccttc ttttattagt ccgctaagcc ctaatgcaga aacgttttcc
     5761 atatttgcaa aagctgaccc taatcctata aatactcctg ctacaacaag taatataaca
     5821 ggtatcatca ttgctccaga aagtacttgt aatttatcct tcatagtatt atttcctcca
     5881 tttttattga aatgccaggc tatataatgc atatatatat acttctattg ctttgtataa
     5941 tgtatctata cttatatttt catttgcctg atgtgaattt ggattatccc cctctattgt
     6001 gggtccaaat gcaactccat tttttaacac tcttgcataa gatgcagctc cttttgtaaa
     6061 aagttcagct tcttttccaa aaccttgttt atatgcattt gataatttat ttattaattc
     6121 actatctggt tttacataca gtaattttaa atccttatat atatcaacta ttaaattttc
     6181 tttttttcca atttcattaa ttctattagt tataaactcc caacttatac ccttaggata
     6241 tctaaagtct attttcatat tcaattcatt tttctctaag aagatactca ttatacatat
     6301 tgtagaaacc cccatatcta catcttcttt atatatccct aatttttttc catgattatc
     6361 atctgtaaag tatgaattta aaatatccct tattataacg cctttatcat ttagtttttt
     6421 aattttctct aaatcttttg ctaaactaaa tgcacagtta tatgacctgt gtggatttct
     6481 cgataaagcc ctttctcctg tatatcttac taatacttta ccttcttcta gttcttctat
     6541 ctctgtataa taagctaatg attctactaa atctttctta tcattagttt taaaaacagc
     6601 ctcaattttg ttacatacaa atccatcttc tttgtttgac tttatatcaa ctaaattgtg
     6661 atttctaaat ttatctttat ctattttctt tcttagattg aagtataaaa tacctttttc
     6721 tccatttact attgggaagt ttccatctgg agaaaatgca aattttggct gttcattgac
     6781 actaaagtaa tgctccatac attcccaagt cgtctcttca gctccaccca ctatcaatct
     6841 aatctttctc ttcggctttt cattaagttc tcttaagaaa agtaatgcat ataaaatccc
     6901 tatcaaaggt cctttgttgt cattaactcc tctaccataa agatagttat ctttttgaca
     6961 tactttaaaa ggtttactct tccaaagttc tttattatat attggcacca catctatatg
     7021 attaagcacc cctactactt cttctccttc tccatattct atatgaattg catatccatc
     7081 aaagtctttt acaacaaaat ctttgtcttt tgctatttga ataagcttat cgaaggcatt
     7141 tcttatttct atcccaaatg gttgatttat atctgttgta ctttcatctc taattgaagg
     7201 tatttcaact aaacttctaa tatcatttat gatattttct tgattggatt ttaaataatc
     7261 aataattagt tctttgttta ctatcatctt aattatttat caccccttta tattaatttt
     7321 acaaagcatt tcagttcata tctacattat aaattttgaa tattattttt tcaatatttc
     7381 aaatacttct ctaagttacc ataatgatac ggtaaacaat caacagatta tgacaaaatc
     7441 atactatacc tattactaat attttaactt atctattacg tcttttttaa tgtaagttta
     7501 ttccaaaaat acataaaaaa agatgtagcc ttagctacat cttcttatat ctattcaact
     7561 tccattatta tgctattaat atctacattt ccaggttcta tcctatcttt tactgttctc
     7621 ttaacagaat cagcatcatc cattataact acaggagtta ttatatttaa atcatttgac
     7681 gttataaaat ctaaatctac ttccattatt aaatctttag cttttacctt atctcctgcc
     7741 tttaccttaa catcaaacgg ctttccttct agtgacattg tctctaaacc tacatgtatt
     7801 aatatatttg tattatcatt actcttaatc attattgcat gctttgtatc aaatacattg
     7861 actatttcac catcaacagg agaatataac ttaccttcac ttggtattat tgctactcca
     7921 tctcccatca tcttttgggc aaaaactgca tctggaacat cttctaagtt cactaaattt
     7981 cccttaaaag ggctatatat tttctcaatc ttattttctt ttttcttaaa tatattaaac
     8041 ataacacact ctccacatta taaaatttta tgataatatt tttcttaaag cttttgctac
     8101 accatcattt acattagtat cagttacaaa atctgccata gattttactt catctgtagc
     8161 atttcccata gcaataccaa gacctgcaaa tttaatcatg gatatatcat tttcattatc
     8221 acctatacaa ataacttctt cgctatctat acctaatata tcacaaaatt ttttaactgc
     8281 aatacctttt gaagtacctt tgctcataac ttcaaaattt atctttccag aacttactac
     8341 ttctaaatct tcttgttttt taaattcttc ctttgcttct ctcaaagaat ctacgttttc
     8401 tgagaaaagt atacatttta aaatttcatt atcagctttt tctaataaag tttcacaact
     8461 tgcattttct attatttcaa ttctgtcttc ttctttattt ttactattta ttaatctata
     8521 tggatgttct tcacctattt cattgttgga tataactatt ttacagcctt taaaatgttt
     8581 gtttaaatta tacttttcac ctattgtata gattttcttt agagtttctt tgctcaaaac
     8641 cttcttatac tcatatccca gtaatttaaa atatgtccca ttagttgata taatatatat
     8701 atcatcccct aaaacacttg taaaatattt catagcatta taaggtcttc ccgtaactaa
     8761 ggctaccttg ataccctttt ccatgacttc ttttagtgct ttaatatttt cttctgatac
     8821 ttcaaatcct ttacccatca aagtaccatc catatcagta caaattaatt tatatcgcat
     8881 ttgtatcaca tcctagtaag atttttctaa tatttcaaca aggtcttctt tctttccaaa
     8941 aggtaaaaat cctaaaactg gacagcttgt tatttgttct aattgttcct ttgttacatt
     9001 aaactcagac atttttgtct taagtcccac tctctttaaa aaactttcta tttcatttcc
     9061 taaagtttta gctgcttcat catcttccac tttttctaat tcttggttta gcattctcgc
     9121 tactttggca aatttctcta tattatcctt atagaacttt tctgcaaatt ttgggaacac
     9181 tatagccaaa gcctcaccat gaggaatatg tgtaactcct cctattattt cacttagtgg
     9241 atgtggtgct gctgctccag catttgccag tgaacttcct cctaatgtat ctgctactgc
     9301 taactttgtt ctgtattcta tattattttt atcttccatt actttaggta gatagttaat
     9361 gactaattcc atagattttt cagacattat ctctgtaaaa tcattagcat ttggatttat
     9421 ataactttca aatgcatggg taaaagcatc aaatgctgtt gatgcagtaa ttctctctgg
     9481 cagagttaac ataagctctg ggtcaactat acattctttt gagaaacaat tttgatgaaa
     9541 tattgtattt ttctcgcttc ctctcgatat tactgctgct tgtgtaactt ggcttcctgt
     9601 tccagaagta gttgatacag ctattaacgg taattctttt tcacttacct tatcgtatat
     9661 tttaaatgga tttgtatatg tactaaacat ataatcccaa tctatctttt ctaaaccaaa
     9721 agtcaatgct aatacttttg cagtatctat acttgaacct cctccaacag ctaaaacaac
     9781 atctacagat tcttttttag ctaattcaaa cccttcattt attaattcta cagttgggtt
     9841 tggctctact ctatcaaagt gtacaacttc tatacctttt tctattaaat tcaatgtaac
     9901 tcttttatat aacttgtcta atggctctac atttggagta gtaactaaca aacatttctt
     9961 tccatatttt cctattattc taggtaattc acttaaagtt ccttgtccaa aatttattct
    10021 tgatggttga taaaagttaa tcatattttt cctcccttaa attattgcct tcatattcaa
    10081 attatacata tcataggatt tttaaggcaa taccttcact gttaactctt atttactaaa
    10141 gcttatggca attattagac aataaatgtt ttattattac catatcatta tgtaaatttt
    10201 attaagtacc catgttataa ttttataatc aatcttattg tataattgac ttaataataa
    10261 aaatagagta tatacacttt tttatgacaa atagcttaaa ggagaattaa attatgaaat
    10321 atttgataga cagtgctaat cttgatgaaa ttcaaaagtg tttaaagtat ggatttaagg
    10381 gaataacagc taatccatct atgtatctta aagagaaagt aaacttccat gaatttattg
    10441 agacatgtgc taatttaaat ccagaaatat taactgctga agtcattgga aataatttat
    10501 acgaattaat taattcatgt gataagatat taaatataaa taaaaacact ataataaaaa
    10561 taaatttctc tgaagttggc ttagaactta taaacatatt aaatagtaaa ggtgtaaaaa
    10621 ctgcatgtac cttgattttc aatgtaaatc aagcatcatt agctataaat gcaggtgctg
    10681 attacctatt cccttttata ggtagaaatg atgaaaatgg gaatgatggt ataaaaaacc
    10741 ttacagaaat atgtaacctt atatcaataa atggatataa tactaaagtt gttgcagctt
    10801 ctattaaaaa tgtattccac ttaactcaag catctctttg tggatgtgat tatgcagctg
    10861 taacatatga tttatttaaa aaagcttctt ttaaccagct tacaatagat ggtgccaata
    10921 cttttgaaag agattggtcc aaggttaata aatagtaact aagatttagg agggtttgca
    10981 ttgaacaaaa gattattaga tattgctgaa ttattactca acagctcaga ctatattact
    11041 gtagatacta ttgccgaaga attaaaagtt agtaataaaa ctattcgtaa tgaccttgtt
    11101 attttagatg aatggctttt agagtttaat ttatccctag ataaaaaaac tggttcaggg
    11161 gttattattc taggtaatga agatataaaa cttaaagtta ttagagatat caatgataaa
    11221 tcaaattgta tatatgcata ctctcctgaa gatagaaaaa ggtatattct aagtaaatta
    11281 tttattaaaa attctaagtt taggattcgt gatatttgca atgaactaca tgttagtaga
    11341 gctactgtgc ataaagactt ggtagttatt caaaattttt tgcaaaactt taaagtgact
    11401 ctagttagaa aaaccaataa tggagtatat ttagagggaa aagagaaaga tattcgaaaa
    11461 gcaatttttg aacttgctac tgttaataaa agttatactg aacttaaaga aatattattc
    11521 tcgaaatcta atgaatgcaa agatacccca tcaacaaaaa tatttaagga gctatttaac
    11581 tatgattttg agaaattatc tcaaatcact ctacacacct tacaagtaga aaaaagtaac
    11641 cttagtgatg agtattatat aaacttctta atacatatag ctatatgtat taaaagaatt
    11701 tcaattaaaa gatatattaa cttatctgaa ggttttttta gagaattatt agaacatgaa
    11761 tactttgaaa aatcaaaaat tttatgtgct aacctaagtg aagcttttga cgtagaattt
    11821 attgatgaag aggtctgtta catccttctt catatagaag gattacttaa atcaataaag
    11881 aaaactacta ctcttgaacc tcttaaaaat gaagaaatta cagagtataa tctatctgaa
    11941 gcaatagctg ttcattgggg aagtatacta aatttaaact taaaggacga tttaatatta
    12001 ataaagtctc ttgctaatca ccttaaatca gttctacaca gaataaacta tgggtttact
    12061 attacaaatc ccatattaaa tgatataaag agaacatttc cttatactta taaggctgct
    12121 aaagagtcta atactgttat caaacagttg atgaattatg aagtcaatga tgacgaaata
    12181 ggatatcttg ctttatattt aatatcagca atagatagaa gcaaatctcc cctaaatact
    12241 attttaatat gtcactgtag tgatggtgta tctaatttat tagtccaaaa gttaagtttt
    12301 gaatttaatc agattaatat agttaaatct atacctctat cttctatttc atttacgaac
    12361 tttgatgatg tggacttaat actaactact gctcctgtag actttgaaca taatgctgaa
    12421 ctgataaata taaatgctct tttaagtaag aatgatattt ctagacttag tacaattata
    12481 aaaaaactat attctgaaaa aaataaaata ttatcttata aataaacaaa aataggcttg
    12541 tctcaacata gagataaacc tatttttcat tgtccaaatg ataaaaatat atttgatttc
    12601 atagaaaaag tgcctcatga actaaaaaat tcattttgag acaccttcat tttttatatt
    12661 taatcttttt atttaagctt ttatattttt taaactttta tatttaaagt aaattttatt
    12721 atttttattg agcacaagca ttatttatat tattaatttc ttcagtattc ttaccaaact
    12781 ttgtagttac aaatcctatt gtttttatta tgagtaataa ggatatcaat gaatatatag
    12841 atatcataaa attcttactt attattccag acgctactat tataaatcca tctagcataa
    12901 ataatataac tggcaacttc agaaacttaa ttaccttatt aataagcatt gcaactaaat
    12961 cagttccacc agtactacag ccccttatta ttgctatccc tatagcagtt cctaccaata
    13021 acccacctat cactcctgct actaatagat tgtcagttat gtgagtttgt ggtattactt
    13081 ttaagaataa tgggcccata agagttatat ataatgtttt tattccaaaa cctttcccta
    13141 aaaataataa tcccattact aaaagtggca tagatattcc taatgatata ttctcaacag
    13201 gcattgataa gaatgtgcta agtacgactg ataatcctgt tatacctcct ggagctaatg
    13261 tatgttctgc aaagaaaaaa ttaactgcta atgccattaa aaatgctgat attgttatca
    13321 gtaaataatc ttttatcatt tcttttttca tttaataacc tcctatgatt atattgtttc
    13381 agtataaaaa atcaattaaa ttagtctagc ttcctgtagt ttttcataaa attaatgctt
    13441 ccttaaaaca ttaagaaagc atctctttta tcatagttta ttatcttatt ctaaaccaaa
    13501 ctttgtagct gcttcatcta tagcttttct acaattctta acacctgctg ttataccatc
    13561 tttatccttg aatattgctg aagataatat tacaacgtct gggttacatt ctattaatgg
    13621 ctccatattg tctgatctta gtccaccatc tattgctaat tcacacttag gattcttttc
    13681 gtctatcatt tttctagctc tttttactaa atcaacagca ctctttctcc atccccagtt
    13741 atcttttcca tcagtttcat caacaccatg aacgactatt tgcaatctgt ctatatcata
    13801 tatagcttca tcaacaaaac ataatggtgt gtaacatcca attgttaatc ctactttcat
    13861 accaaattct ctacagtaat ttattatgta tgcaagagga gcacctaaaa aatgttctgc
    13921 tggtattatc aacaagttac atcctgctgc tgctattttt tctataaata atctatcaca
    13981 atctcttgtg tatatatggc attctattgg tttatctgtc actggtctta ttgcttctat
    14041 tatttgatga ccacccataa gttgcatatt ttttaagtca tgcatatctg ctgcatctga
    14101 atgtacgtag tctacacctg catccattgc ttctttaact acggctgata tatgaccgta
    14161 atttacatgt gctaatcctg ctgctatttt tatgtgtttc atatttgtat acctccatat
    14221 tttactttaa ttagtattaa atattagttt ttgatatttt tttaatattt tttaatccag
    14281 tattactttt atttagcatt acttttattg ctgtatatat ttcttttatg aacttattct
    14341 gcttttgtta actgtgaact tattttgctt tgtaaattaa tattacactg atatattttt
    14401 tttcacaaca ctttgactac tccttaaaag tacctttata ttgtggtaaa ttatagacat
    14461 taattgtata taaaaagtta gttttttacc acattcttat ggcatttatt gtctatacta
    14521 actcaattga tatagctagt tcatttaata tataatcaga ctataaatat aaatttactc
    14581 gataaaaagt accattcata acaatattaa tgaaagggag atgtaaaatg agaaactgta
    14641 ttattgccca atctggaggc cctacagctg ttatcaatgc ttctgcagtt ggtatttatg
    14701 aaaaaaattt agaaactaaa tactttgata aagtatattt tggtatcaat gggattgaag
    14761 gtatattaaa caaaaatata atagattcag actccttaaa caaagataat gtattaaatt
    14821 taaagcaaac accttcgtct ggtcttggtt cttgtagata caaactggct gattataaaa
    14881 taaattctta tgattatgat agagttataa atatactaga tgaatataaa atagatacat
    14941 tattttatat aggcggaaat gattctatgg acacagtgat gaaattttct caatactgca
    15001 aaaacactac ttgtaaaata aaatttatag gtattcctaa aacaatagat aatgatttat
    15061 taggaactga caactgtcct ggatttggtt ctgctgcaaa gcttatttct acactaataa
    15121 tggaaacata cttagatttt tctatataca aaaataatgg tatatttata gtagaaacta
    15181 tgggtagaga tactggatgg ttagcagctt cttcttcact agctagactt aatgatactc
    15241 cacttgttga tttaatatac cttccagaaa ttgatttcta tgaagataag tttatagaag
    15301 atgttagaaa aatatttaaa gaaaaaaata aggcttatat agttgcatca gaaggtataa
    15361 gggataaata cggcaacttt atatgcgaac aaaaaacttc tgctcatgat aaatttggtc
    15421 attcccaatt aggtggagta agcaactatc taaagaatct tatagtatca tcatcaatta
    15481 caacaagagt aaaaacctta gaacttggtg taatacaaag atgtgctatg catttagctt
    15541 cagatactga tttaaaacaa gcagctgaag ttggtgcata tgggatagaa ctatctaaaa
    15601 aaggatatac aggagttatg gctatcatta atagaacatg caataatcct tataagtatg
    15661 aagttagtca tactgatatt tctaaaatag ctaacaagac aaaatatttt cctaaagagt
    15721 ggataaatga agaatgtaat tatataacta atgaagccct aaattatcta tatccactta
    15781 ttcaaggtaa accagatatc aaatataaaa atggactacc ttatttttca attatttact
    15841 aggaaattat tataatttga tatacactca attctatcgc tcttaaatac ctaagtggct
    15901 gttaaataaa attataaatt gttatatgac agtcacttaa tcgtttaatt atttaactgt
    15961 ttagtttttt aattttttaa tctttttaat actaatacaa tttgttgtaa taatatctca
    16021 cacccatttt cattttggat agattataat tagttagaca ataaatattt tgtcatgata
    16081 ttattatata tagtgataca aatagaaata cttttatcat ggctagaaaa ataaagatta
    16141 cacaaaggat ttaaaagtca aacaacttca attaaaataa atggttaaaa taacttctaa
    16201 atcaactaca taaacaataa aagaacataa agtagttaaa tctacaatta taaaattagt
    16261 aagactataa taacttaaat aaaattaaag ttaacaaaga ctat
//
LOCUS       DAELLV010000069        16270 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.069, whole genome shotgun sequence.
ACCESSION   DAELLV010000069 DAELLV010000000
VERSION     DAELLV010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16270
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.069"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            61..1434
                     /locus_tag="KPD58_001773"
     CDS             61..1434
                     /locus_tag="KPD58_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0265543.1"
                     /translation="MNEILENIDEGVHFVNKDGKTIIYNNSVQKMEKMNRHDILTNSF
                     FDIVNKMKINRSTLLEVLEKREVIKDNIQKYLDKNGKEITAINTTIPIDIKGEFVGAL
                     EISKNMTTIENLLNRNYKKNLVNKVSSRQKKGYEFDDILGESNLIKEAIAKSKKACKS
                     DASVFIYGETGTGKELFSQSIHYGSNRKNYPFIAQNCAALPESLFEGILFGTSKGGFT
                     GAIDRPGLFEQANKGTLLLDEINSMPIMLQAKLLRVLQEGYVRRVGGTKDIPIDVRII
                     ATTNESPKVILNEHKMRKDLYYRLNIIHIKIPPLRDRKEDIPLLCKKFISKYNKKLNK
                     KVVGISDEAIKILKNYDWQGNIRELENIIYCTMSMMEDEVQIETSMINLNDYYNLKEN
                     KESNNYSLNDLEDKTLSKIISEIEEKYIYESLEKASYNITKAASILGMNRQNLQYKIK
                     KYNIKIL"
     gene            1690..2757
                     /locus_tag="KPD58_001774"
     CDS             1690..2757
                     /locus_tag="KPD58_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-binding protein"
                     /protein_id="HBG0265544.1"
                     /translation="MRKVRVGIWGFGAMGIGMANMILKKEGIEIVSVCSRSTSGKSMY
                     NVLGIERGERPEVIINKNYEEVFREKSVDVVLLATDSFTKKAFDKIIFLLNRKINVIS
                     TAEQMAYPQADDADLAKKMDEVAKENGVSILGTGINPGFVLDLLVLALSGTCEEVTSI
                     KAKRVNDLSPFGKSVMVEQGVGVTREEFIKGVEDKTIAGHVGFVESINMIADGLGWKL
                     DKIEQTKEPIMTTVDRKSKYGEALAGNVAGCRQCGYGYVNGEVLIEMEHPQQIIPEAE
                     GIKTGDYVSIKGIPNIDLQINPEIPGGVGTYAMIVNSIPLIINARPGLKTMLNIPVPR
                     AIMGDIRNQIEVELEEESKAN"
     gene            2878..3186
                     /locus_tag="KPD58_001775"
     CDS             2878..3186
                     /locus_tag="KPD58_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-ketopentanoate thiolase"
                     /protein_id="HBG0265545.1"
                     /translation="MDAKINDWVIIHNIVLTPEERAPQVPEDTKKVSLEMWVKGFIQS
                     DASIGDLVEVKTITGRLVKGNLLKINPYYTHDYGKCIPELLQIGIQAKEILFGGVYNE
                     "
     gene            3179..4609
                     /locus_tag="KPD58_001776"
     CDS             3179..4609
                     /locus_tag="KPD58_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent lyase/thiolase"
                     /protein_id="HBG0265546.1"
                     /translation="MNNSTSMKDMSYQAVMGRNNEIMKNAIGLDYSSFEQEGIGFDYE
                     KMMSETGYTLQDIEAIQSQYAVGNTPLIELKNLTKLARKCAKEGKGARIFVKDEAMNA
                     SGSFKARRAATAVYHAKQMGYKGVIAATSGNYGAAVASQAAMQGLKCIIVQECYDSNG
                     VGQPEIIEKARKCEAYGAEVVQLTVGPELFYTFLVLLEETGYFNASLYSPFGIAGVET
                     LGYELAIQFREKYKKDPDIVVCTNAGGGNLTGTARGLIKAGSINTKVVGASVDLKGLH
                     MASDNQFNKKSFTTGHTGFGIPYCTWPDRSDVPRSAARPLRYMDRYVLVKQGEVFYTT
                     ELLAQLEGIERGPAGNTSLAAAFSLAQELDEDKAIVVQETEYTGAGKHICPQLTFARE
                     NGIDIKFGNPREEIAGVNLILPERPELLKCVDIDMNKIRKSFIKNCISNNHIDNIDKL
                     SNRDIEFLMEEVKSSRDFVVDVLNNL"
     gene            4687..5061
                     /locus_tag="KPD58_001777"
     CDS             4687..5061
                     /locus_tag="KPD58_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HBG0265547.1"
                     /translation="MKKREDDFEVRRKHLQELSDDELKERFWSLATQIVEPMIELGKK
                     NTTPSIERSVLLRMGFSSLEVKPILEGVMERGLIGKGAGHVVYKLAKSKNITVREAGL
                     LLVKGEYWDEVTCLFREGVESC"
     gene            5055..7259
                     /locus_tag="KPD58_001778"
     CDS             5055..7259
                     /locus_tag="KPD58_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892628.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HBG0265548.1"
                     /translation="MLKENKKLDIDYILKDLDKYKPKRRGWVWREHLENLEMGPFKYK
                     DCTKPLKKSVGLPSSKYFNNIDPQPSPVITTEIASGRFEDDIRRMRMAAHHGADHLMV
                     IRTAGQSHFDGLIEGTPQGIGGVPITRKQVRAQRKALDFIEEEVGRPINYHSYVSGVA
                     GPEVAVMFAEEGVNGAHQDPQYNVLYRNINMVRSFVDACEAKKIMAFANIAQIDGAHN
                     ANATAREAWKVMPELMVQHALNSIFSEKIGIDKSNICLSTVPPTAPPAPCLKIDLPYA
                     VALRELFSDYKMRAQMNTKYMESSTREATVTHVLNLLTSVLTRADIQSTITPDEGRNV
                     PWHIYNIEACDTAKQALVGMDGLMDMIELKDVGELRDKARELKERAVLYMEEISEVGG
                     YFESVEQGFFVDSGNYPERNGDGISRKIKGGVGEGTVYEREEDYLAPVTAHFGYNNVA
                     QYDENAIDNPSMLIDGCTFENPDKIIYIDELDDFDNVENRLKESYEYRNGTKIKPEME
                     WCADGIVMITMMLPTDKRTAEFAALEFVKKMNLQEIEVISREVMHESEGTRIEVKGRV
                     PFDIDLNNLVIPEEPKVLTDEEIRADIEEKPMKIVSATVGEDEHSVGLREIIDIKHGG
                     IEKYGIECHYLGTSVPVEKLVDAAIELNADAILASTIISHDDIHYKNMKKLHDYCVEK
                     GIRDKVMIACGGTQVTPEIAVEQGIDAGFGRNSKGIHVATFLVEKRREMSNK"
     gene            7259..8668
                     /locus_tag="KPD58_001779"
     CDS             7259..8668
                     /locus_tag="KPD58_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HBG0265549.1"
                     /translation="MENSKVSLKIDVLVAEIGSTTTVVNAFHDINTNNPIFLGQGQAP
                     TTVFEGGDVRNGLSGAIKDLANKLSVDDIKYNDMFATSSAAGGLKMTVHGLVYDMTVK
                     AAKEAALGAGAVIRQITSGRIKRTDLNKIKEINPNIILIAGGVDYGERDTAIYNAEMI
                     ASMNLGIPVIYAGNVENQEEIRLIFEDTNYKLYITENVYPKIDLLNIEPTRRIIQSVF
                     EEHITTAPGMKYIKEMVNENITPTPGAVMEASKLLYKNIGDLLTLDVGGATTDVHSVT
                     DGSDYINKILVNPEPTAKRSVEGDLGVYVNMKNIVEVIGKENLQSELSIDIDAVIENY
                     PPIPKSKEEILFVERLTKEAVVKAMLRHSGKIRNIYGTTGKVKIAEGKDLTEVRYIVG
                     TGGALTRLPSRIEILDNMLKYNKNNELLFPKEKTKILIDNDYIMASMGVLSKKYEEAS
                     LKLLLKSLNFEEERLCILG"
     gene            8653..9717
                     /locus_tag="KPD58_001780"
     CDS             8653..9717
                     /locus_tag="KPD58_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HBG0265550.1"
                     /translation="MYPRLEIDIEKLKHNAKLISQMCHERNIKISFVTKSFCAQKEIV
                     EEICSEGIDHIADSRIQNLKNLQDINLPKILIRIPMLSELEEVINYCDISFNSELGTI
                     KKLNELCESKNIIHKIVLMFDLGDLREGYFYEEDFFSNVREIVRLKNIEIIGIATNLT
                     CYGAIIPSRENIGRLVSIAKRMEEKFGINLEIVSGGNSSSIHLLINNNMPEGITNLRI
                     GESILLGRETAYGENINGTYQDAFKLICQVVECKEKPSVPIGEIGVDAFRNKPVYEDK
                     GILKRAIIAIGKQDINIDSLIPIDTDIKILGASSDHMILDVSNTKYDYKLGDNLEFLL
                     TYGGIMSSSTSKYVSKKIIV"
     gene            9747..11168
                     /gene="nhaC"
                     /locus_tag="KPD58_001781"
     CDS             9747..11168
                     /gene="nhaC"
                     /locus_tag="KPD58_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBG0265551.1"
                     /translation="MKERECRKASLFDAMIPITCLILFLSLGVLVYGSSPHVPLIGAA
                     AVAGLVAVYRLGFKWKELELSMFNSIKMAMQAILIIIIIGVLIGTWIVSGVVPCMIYW
                     GLKILSPNIFLVASTLVCAIVSLATGSSWSTMGTVGVALLGIGQSLGMPIGLIVGSII
                     SGAYFGDKLSPLSETTNLAPAMAGTDLFTHIKYMLYSTIPSLLICLVIYGVIGMKYSG
                     QALDIKQIELIRSTLDSTFNTLSPVLLLAPAIVIGLVVFKVPAIPGLIVGAVLGVLFA
                     VVFQGESMNTILDAAQNGYVSSTQIQEVDALLSKGGIMNMMSTVSLTICALSLGGILE
                     KTGMLEVVASSLLRLAKGVFGTVLCTMVTCTITNIIAGEQYLSIVIPGRMYNKEYKKR
                     GIHPKMLSRALEDSGTLTSPLVPWNTCGAYITATLGVSALTYGPYALLNIINPIVSLV
                     LIACKFKIARIEDEPETCLNFDV"
     gene            11597..12061
                     /locus_tag="KPD58_001782"
     CDS             11597..12061
                     /locus_tag="KPD58_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBG0265552.1"
                     /translation="MTRISNITIAEQSEYCFLAIRKTIDFMVEFSEFSKQSFEKISKY
                     LEERGILSSGAPIVCFHNMDLAKLDVEVGFPVATYIDGKNEILQRIVPAQKIITAIDL
                     GPYELQDPTLEDLFSWANSNGYKLHGDIYYQYLNDINRPASEYLTKMMLPII"
     gene            12295..12657
                     /locus_tag="KPD58_001783"
     CDS             12295..12657
                     /locus_tag="KPD58_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG0265553.1"
                     /translation="MKIYDILTRFYIQDIEKAIPFYENLLKEKCSLRFSYKEVGLELA
                     QIGNVLLLSGSDDALKPFIETKSTFMVDSVDEWRSYLLDNGAVVVRDKKKVPTGYNMT
                     LRHPDGTIIEYVQHTKQD"
     gene            13252..14088
                     /locus_tag="KPD58_001784"
     CDS             13252..14088
                     /locus_tag="KPD58_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0265554.1"
                     /translation="MSYKVINVITAIDYIEEHLSEKLDLDIVANAVHYSKYHLHRTFT
                     TSVGLTMHDYIKRRKLTEAAKLLAFSKKPIIEIALIAGYESQQAFTSIFKAMYKKSPN
                     KYRKEQEFYPLQLRFVLKKDNLFSKNVLELEKEIKLASMSDIPLWMNLVRLVIDGFPN
                     LQEEEYIYQLEQYILEERALILKLNNVAIANMVFNRETRSIDFFGIHPQYRNSDIAQV
                     FLKKVIEDFLIDTDISITTFREGDKADTGHRDMIKKLGFAEAELLVEFGYPTQKFILP
                     LS"
     gene            14172..>16270
                     /locus_tag="KPD58_001785"
     CDS             14172..>16270
                     /locus_tag="KPD58_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HBG0265555.1"
                     /translation="MSENKQVVNCDIIASNSKHNMCEYDAELLTFFPDEIAHLIEINN
                     KLDNAFKKAENLVDKLDKDYMDAKMYMVKNRGEIDPHEMFQNEQGLKQIDNYGAFMVK
                     VRDKINKIKDSPYFARIDFRLKDMDDESKYYIGRFAFDYEDELIILDWRSPIASMFYD
                     YEIGKAGYDAPIGWVDGEITRKRQFKIKNGKLEYALESSINIQDDILQKELSHTSDEK
                     MKSIISTIQKEQNQIIRNDKADTLIIQGVAGSGKTSIALHRIAFLLYRFKDKISANNV
                     IILSPNKVFGDYISNVLPELGEEPLCELSFENIAEVQLDRVINFESEKDPLEINDAKW
                     SERVRFKSTLDFVKLIDDYIKQMPNKIFIPKDYTFGSFTAKSDWIQSRFEAYNRYPVK
                     KRLEKVAEDIHYKFESDNIMEEDLPKVKSILKSLNGMLTIKNTLTLYKDFFKQMNISN
                     MFVMATKKTLEWSDVYPFIYIHAAYEGIQEDKIIRHVVIDEMQDYTPIQYAVINLLFK
                     CKKTILGDFGQLVNPNHTHTLDDMKQLYNDGELVMLNKSYRSTFEIINFAKKIQDISS
                     LEPIERHGEEPALLKCNNEQDEINKIKIEIEEFKKSDNATLGIILKTDNDAKAVYDAL
                     REKYSVHLISSESSSFTKGVSITSIKMSKGLEFDEVIVPSVNNKTYYSDYDRSLLYIA
                     CTRAMHKLKLTYTGELT"
BASE COUNT         6430 a         1811 c         3080 g         4949 t
ORIGIN      
        1 gtttttatat atttaggtac tggggtgata tagttgaacc aagagatatt ttttaaaata
       61 atgaatgaaa tattggaaaa tattgatgaa ggggtacatt ttgttaataa agacgggaaa
      121 actataatct acaataattc tgtacaaaaa atggaaaaaa tgaacagaca cgatatttta
      181 accaattcat tttttgatat agtcaacaaa atgaaaataa acaggagtac actattagaa
      241 gttttggaaa aaagggaagt tatcaaagac aatattcaaa aatatctgga taaaaatgga
      301 aaagagataa cagcaattaa tactacaata ccaattgata ttaaaggcga gtttgtaggg
      361 gcacttgaga tatcaaagaa tatgacaacg atagaaaatt tactaaatag aaattataaa
      421 aaaaatttag tcaataaagt tagtagtaga caaaaaaagg gttatgaatt tgatgatatt
      481 ttaggggaaa gtaatttgat taaagaagca atagctaaat ctaagaaagc ttgcaagtct
      541 gatgcttcag tatttatata cggtgaaact ggaacgggta aagagttatt tagccagtcc
      601 attcactatg gaagtaatag gaaaaactat ccatttatag cacaaaattg tgcagctttg
      661 ccggaatcac tattcgaagg tattcttttt ggaactagca agggaggatt tacaggagca
      721 atagataggc caggcttatt tgaacaagct aacaaaggga cattgttatt ggatgagata
      781 aattccatgc ctattatgtt acaggctaaa ctattaaggg ttttacaaga aggttatgta
      841 agaagggttg gaggaactaa ggatatacct atagatgtaa gaattattgc aacgacaaat
      901 gaaagtccaa aggtaatttt aaacgagcat aaaatgcgaa aagatttata ttataggctt
      961 aacataattc atataaaaat tcctcctctt agagatagaa aagaggacat acctctattg
     1021 tgcaaaaaat ttatttcaaa gtataataaa aagttaaata aaaaagtggt aggaatttca
     1081 gatgaagcta ttaaaatttt aaaaaattac gattggcaag gtaacataag agagctagaa
     1141 aacataattt attgtactat gagtatgatg gaagatgagg tacaaataga aacaagtatg
     1201 attaatctaa acgactatta caatcttaaa gagaataaag agagtaacaa ttattcttta
     1261 aacgatttgg aggacaaaac acttagtaag attatcagtg agatagaaga aaagtatata
     1321 tatgaatcat tagaaaaggc atcttacaat ataactaaag ctgcatcaat tttaggtatg
     1381 aataggcaaa atttacagta taaaataaaa aaatataata tcaaaatatt ataaataagc
     1441 aagataaaaa aacaaaatca aagtaaaata aaaaagcaaa atagaggagc aaaattatta
     1501 taaaagagca aaatattttg ctcttttttt tatttctcat aagaaatatt attttttatt
     1561 tatgaaaata cataataaaa taattatagt ttcaacgttc caataaatat ttactttaat
     1621 tttaaagttg gcacgattta tgctttaact aattattgag aatatataaa tttttaggag
     1681 ggtgtatata tgagaaaagt aagagtagga atatggggat ttggagctat gggaataggt
     1741 atggctaata tgattctaaa gaaagaaggt atagaaatag tatctgtttg tagtagaagt
     1801 acttcaggaa aaagtatgta taatgtttta ggaattgaaa gaggagaaag accagaagtt
     1861 attatcaata aaaattatga agaagttttt agagaaaaaa gtgtagatgt tgttttgtta
     1921 gcaactgatt catttactaa aaaggctttt gataaaataa ttttcttatt aaatagaaaa
     1981 ataaatgtta tatcaacagc agaacaaatg gcatatccac aagctgatga tgcagattta
     2041 gctaaaaaaa tggatgaagt tgcaaaggaa aatggagtaa gtatacttgg aacaggaata
     2101 aatccaggat ttgtattaga tttactagta ttggcacttt caggtacttg tgaagaagtt
     2161 actagtataa aagctaagag agtaaatgat ttatctccat ttggaaagtc tgttatggtg
     2221 gaacaaggtg ttggagtaac aagggaagaa tttataaaag gtgtagaaga taagactata
     2281 gcaggtcatg taggttttgt agaatcaata aatatgatag ctgatggatt aggttggaaa
     2341 ttagataaga tagaacaaac aaaagagcca ataatgacta cagtagatag aaaatctaag
     2401 tacggagagg cattggctgg aaatgttgct ggttgtagac aatgtggata tggatatgta
     2461 aatggagaag ttttaattga aatggagcat ccacaacaga taataccaga agcagaaggt
     2521 attaaaacag gagactatgt atcaataaaa ggtataccaa acattgattt acaaataaat
     2581 cctgaaatac ctggaggagt aggtacttat gcaatgatag taaactctat tccattaata
     2641 ataaatgcta gaccaggtct taaaactatg ttgaatatac cagtacctag agcaataatg
     2701 ggagatataa gaaatcagat agaagttgaa ttggaagaag aatcaaaggc taattagtaa
     2761 gatagcaact agctaattag taagataaca gttagctaat tggtaaaata atgtttaatt
     2821 taaaataaca gtaaattaat tagtaaggca atagtcaatt aataagggga tgaaaaaatg
     2881 gatgctaaaa ttaatgattg ggttattata cacaatatag tattgactcc agaagaaaga
     2941 gctccacaag ttccagagga tactaagaaa gtatccttag aaatgtgggt taaaggtttt
     3001 atacaaagtg atgcttctat aggcgactta gtagaagtta aaacaattac aggcagactt
     3061 gtaaaaggta atttacttaa aataaatcca tattatactc atgattatgg taaatgtata
     3121 ccagagttac tccaaatagg aatccaagct aaagaaatat tatttggagg tgtatataat
     3181 gaataattca acatcaatga aagatatgag ttatcaagct gttatgggaa gaaataacga
     3241 aataatgaaa aatgcaatcg gattggatta ttcttctttt gaacaagagg gaatagggtt
     3301 tgattatgag aaaatgatga gtgaaacagg atatacatta caagatattg aagcaattca
     3361 atcacagtat gctgttggaa atactccttt aatagagctt aaaaatttga ctaaattagc
     3421 tagaaaatgt gctaaagaag gtaaaggagc tagaatattt gtaaaagatg aagcaatgaa
     3481 tgcatctggt agttttaaag caagaagagc agctactgca gtatatcatg ccaaacaaat
     3541 gggatataaa ggtgtaatag cagctactag tggtaactat ggtgcagctg tagcatcaca
     3601 agcggcaatg cagggattaa aatgtataat agttcaagaa tgttatgatt ctaatggtgt
     3661 aggtcaacct gaaatcatag aaaaagcgcg aaaatgtgag gcatatggag cagaagttgt
     3721 tcagttaact gttggaccag agttatttta tacgttctta gttttattag aagaaacagg
     3781 ctattttaac gcatcattat attcaccatt tggaattgca ggtgtagaaa ctttaggtta
     3841 tgagttagct atccaattta gagaaaagta taaaaaagac cctgatatag ttgtttgtac
     3901 aaatgcaggt ggtggtaatc ttactggaac tgcaagagga ttgataaaag ctggttctat
     3961 taatactaaa gtagtaggag caagtgtaga tttaaaagga ttacacatgg ctagtgataa
     4021 tcaatttaat aaaaaatcat ttacaacagg acatacaggt tttgggatac catactgtac
     4081 atggcctgat aggtcagatg tacctaggtc tgcagctaga ccacttagat atatggatag
     4141 atatgtatta gtaaaacaag gagaagtttt ttatacaact gaattattgg cacagctaga
     4201 aggtatagaa agaggtccag caggaaatac atctttagca gcagcattct ctttagctca
     4261 agagctagat gaggataaag ctatagtggt acaagaaaca gaatatacgg gtgcaggtaa
     4321 acatatatgt ccacaactta cctttgcaag agaaaatgga atagatataa aatttggaaa
     4381 tccaagagaa gaaattgcag gagtaaactt aatattacca gaaagaccag aattattgaa
     4441 atgtgttgat attgatatga ataaaataag aaagtcattt ataaaaaatt gtatatctaa
     4501 taatcacatt gataatattg ataagctaag taatcgagat atagaatttt taatggaaga
     4561 agtaaaaagt tcaagagatt ttgttgttga tgtattaaat aatttatagt aattattgtt
     4621 ttttaattta atataaaata aaaattcaga aaattcgatt aaatcaatac gaatggagga
     4681 atgttcatga aaaaaagaga ggatgatttt gaagttagaa gaaaacatct tcaagaactg
     4741 agtgatgatg agttaaaaga aaggttttgg agtttagcta cccagatagt agaaccaatg
     4801 atagaacttg gtaaaaagaa tacaacacca tcaatagaaa ggtctgtctt gttgagaatg
     4861 ggattttctt ctcttgaagt aaaacctatt ttagaagggg ttatggaaag aggtcttata
     4921 ggtaaaggag cagggcatgt agtttataaa ttggctaaat ctaaaaatat aacggttaga
     4981 gaagcaggtt tgttattagt taagggtgaa tattgggatg aagttacatg tttatttaga
     5041 gaaggggtag aatcatgtta aaggaaaata aaaaattgga tatagactat atattaaaag
     5101 atttggataa atataaacca aaacgaagag gttgggtttg gagagaacac ttggaaaatc
     5161 ttgaaatggg accttttaaa tataaagact gtacaaaacc tttaaagaag agtgtagggc
     5221 taccatcatc taagtatttc aataatatag accctcaacc aagtccagtt attactactg
     5281 aaatagcttc tggtagattt gaggatgata taagaagaat gagaatggca gctcatcatg
     5341 gagctgacca tctaatggtt atcagaactg ctggacaatc acattttgat gggcttatag
     5401 aaggaactcc acaaggtata ggtggagttc caataacaag aaaacaagta agagctcaaa
     5461 ggaaggctct tgattttata gaagaagaag ttggtagacc tattaattac cattcatatg
     5521 taagtggtgt tgctggtcct gaagtagcag taatgtttgc tgaggaagga gtcaatggag
     5581 cacatcaaga cccacaatat aatgttctgt ataggaatat aaacatggta aggtcttttg
     5641 tagatgcttg tgaggcaaag aaaataatgg cttttgccaa tatagctcaa attgatggag
     5701 cacataatgc taatgctact gctagagaag catggaaggt aatgcctgaa cttatggttc
     5761 aacacgcttt aaactctata ttctcagaaa aaataggtat agataaaagt aatatatgct
     5821 tatcaacagt tccaccaaca gcacctccag caccatgttt aaaaatagac ttaccttacg
     5881 cggttgcact tagagagtta tttagtgatt ataagatgag agctcaaatg aatactaaat
     5941 atatggaatc atcaactaga gaagcaacag ttactcatgt attaaactta ttaacatcag
     6001 tattaactag agctgacata caatcaacta taacacctga tgaaggtaga aatgtacctt
     6061 ggcatatata caatatagaa gcatgtgata cagcaaagca agctttagtt ggtatggatg
     6121 ggcttatgga tatgatagaa cttaaagatg tcggtgaatt aagagacaaa gcaagagaac
     6181 ttaaagaaag agctgtatta tatatggaag aaatatctga agttggtgga tattttgaat
     6241 ctgtggaaca aggatttttt gttgattctg gaaattatcc agaaagaaat ggtgatggaa
     6301 tatctagaaa aataaaaggt ggagttggtg aaggtactgt ttatgaaaga gaagaagatt
     6361 atttagcacc tgtaactgct cactttggat acaataatgt agctcaatat gatgaaaatg
     6421 ctatagataa tccatcaatg ttaatagatg gatgtacatt tgaaaatcca gacaaaataa
     6481 tatatattga tgagcttgac gattttgata atgtagaaaa tagactaaaa gaatcatatg
     6541 agtacagaaa tggaactaaa attaagcctg aaatggaatg gtgtgcagat ggaattgtaa
     6601 tgataacaat gatgcttcct actgataaaa gaacagcaga gtttgcagct ttggaatttg
     6661 tcaaaaaaat gaacttacaa gaaattgagg tcataagtag agaagttatg catgaatctg
     6721 aagggactag aatagaagtg aaaggaagag ttccgtttga tatagattta aacaatttag
     6781 taataccaga agaacctaag gtactaactg atgaagaaat aagagcagat atagaagaaa
     6841 aacctatgaa aatagtttca gctacggttg gagaagatga acattcagtt ggtttaagag
     6901 aaataataga tataaaacat ggaggtattg aaaaatatgg catagaatgc cactatctag
     6961 ggacttctgt acctgtagaa aaattggtag atgcagcgat tgaactgaat gcagatgcaa
     7021 tacttgcatc tacaataata agtcatgatg atattcacta taaaaacatg aaaaaattac
     7081 acgattattg tgtagaaaaa ggtataagag ataaagtaat gattgcgtgt ggaggaacac
     7141 aagtaacacc tgaaatagct gttgaacaag gcatagatgc tggatttgga agaaacagca
     7201 aaggcataca tgtagccact ttcttagttg aaaaaagaag agaaatgagt aataaataat
     7261 ggaaaatagc aaggtttctt taaaaataga tgtgttggta gctgaaattg gaagtactac
     7321 tacagtggta aacgctttcc acgatattaa tactaacaat cctatatttt tgggtcaagg
     7381 tcaagctcca acaacagtgt ttgaaggtgg cgatgtaaga aatggcttat caggtgctat
     7441 taaagattta gctaataaat tatctgtaga tgatataaag tataatgata tgtttgcaac
     7501 ttctagtgca gctggtggat taaaaatgac tgttcatgga ttagtctatg atatgacagt
     7561 aaaagcagct aaagaagcag ctttaggggc tggagctgtt attcgtcaaa taacatcagg
     7621 tagaattaaa agaactgatt taaataaaat aaaagaaata aacccaaaca taattttaat
     7681 agctggtgga gtcgattatg gagaaagaga tacagctatt tataatgcag aaatgatagc
     7741 ttctatgaat ctaggcatac cagttatata tgctggtaat gttgaaaatc aagaggaaat
     7801 aaggttaata tttgaagata ctaattataa gctttatata actgaaaatg tataccctaa
     7861 aatagattta ttgaatatag aacctacaag aagaatcatt caaagtgtat ttgaagaaca
     7921 cataactaca gcaccaggaa tgaaatacat taaagaaatg gttaatgaaa atattactcc
     7981 aaccccagga gctgtaatgg aagcttctaa acttttatat aaaaatattg gagatttatt
     8041 aactctggat gtaggtggag caactactga tgtacattct gttacagatg gaagtgacta
     8101 tattaataaa attttggtta atcctgaacc tactgcaaag agaagtgttg agggggattt
     8161 aggtgtatat gtaaatatga agaatatagt agaagttata ggtaaagaaa acttacaaag
     8221 tgaactatct atagatattg atgcagtaat agagaattat cctccaatac caaaatcaaa
     8281 agaagaaata ttatttgttg aaagattgac aaaagaagct gttgttaaag ctatgctaag
     8341 acattcagga aaaataagaa atatctatgg aacaactgga aaagtcaaaa ttgcagaagg
     8401 gaaagattta acagaagtaa gatatatagt tggtacaggg ggagcactta ctaggctccc
     8461 tagtagaatt gaaatattag ataatatgct caaatacaat aaaaataatg aattactatt
     8521 tccaaaggaa aaaacaaaga tattaataga taatgactat attatggcat ctatgggtgt
     8581 tttatctaaa aaatatgaag aagcatctct taagctcctg ttaaaaagtt tgaattttga
     8641 ggaggaaaga ttatgtatcc taggttagaa atagatatag aaaagttaaa gcataatgca
     8701 aaattaattt cgcagatgtg tcatgaaaga aatattaaga tatcttttgt tactaaatca
     8761 ttttgtgcac aaaaagaaat agtagaagaa atatgtagtg aaggaataga ccatatagca
     8821 gattcaagaa tacaaaactt aaaaaattta caagatataa atctacctaa gatattgatt
     8881 agaatcccaa tgttaagtga attagaagaa gttatcaatt attgcgatat tagttttaac
     8941 tcagaattag gaacaataaa gaaattaaat gaattatgtg aaagtaaaaa tattattcat
     9001 aagatagttc ttatgtttga cttaggcgat ttacgagaag gatactttta tgaagaagac
     9061 ttttttagta atgtaagaga gatagtaaga ttaaaaaata ttgaaataat tggtatagct
     9121 acaaacttaa cctgttatgg agctattata ccatctaggg aaaatatagg cagactggta
     9181 agtatagcaa aaagaatgga agaaaaattt ggaataaatc tagaaatagt atctggaggt
     9241 aattcaagtt ctattcatct gttgataaat aataatatgc cagaaggtat tactaatttg
     9301 agaataggcg agtccattct acttggtaga gaaacggcat atggtgaaaa tataaatgga
     9361 acatatcaag atgcatttaa attaatatgc caagtagttg aatgtaaaga aaaaccatca
     9421 gttcctattg gagaaatagg agttgatgcc tttagaaata aacctgttta tgaagataag
     9481 ggaatcttaa aaagggcaat catagctata ggtaaacaag atataaatat tgactcttta
     9541 attccaatag acactgatat aaaaatatta ggggcaagtt cagaccacat gatactagat
     9601 gtaagtaaca caaaatatga ttataaatta ggtgacaatt tagaattttt attaacttat
     9661 gggggtatca tgtcatctag tacgagcaaa tatgtttcaa aaaaaattat agtgtagtgt
     9721 ttaaataatt aggaggaaac tattttatga aagagcgaga atgtagaaaa gcaagtctgt
     9781 ttgatgcaat gataccaatt acttgtttga tattattttt aagtctagga gtattagtgt
     9841 atggttcatc acctcatgta ccactaatag gagcagcagc agtagcagga cttgtggctg
     9901 tatatagact tggatttaaa tggaaagagt tagaattaag tatgtttaat agtataaaga
     9961 tggcaatgca agcaatctta atcataatta taataggcgt acttatagga acttggatag
    10021 taagtggagt agttccatgt atgatttact ggggactaaa aattttatca ccaaatattt
    10081 ttttagtagc atcaacatta gtctgtgcaa tagtatctct tgcgacaggt tcatcttgga
    10141 gcacaatggg tacagtagga gtagctcttt taggcattgg tcaaagttta ggtatgccaa
    10201 ttggattaat cgtaggttct ataatatctg gagcttactt tggagataaa ctatcacctt
    10261 tatctgaaac gactaactta gctccagcaa tggcaggaac agatttgttt acacatataa
    10321 aatatatgct atattcgact ataccatcat tattaatttg tttagtaatt tatggtgtaa
    10381 ttggaatgaa gtattcaggt caagcactag atataaaaca aatagaatta attaggtcta
    10441 cattagattc tacatttaat actttatcac cagtattatt attagctcca gcaatagtaa
    10501 ttggattggt tgtatttaaa gttcctgcta taccagggct tatagtaggt gctgtattgg
    10561 gagtattgtt tgcagtagtt tttcaaggag aatctatgaa tacgatatta gatgcagcac
    10621 aaaatggata tgtttcgtca acacaaatac aggaggtaga tgctttatta tctaaaggtg
    10681 ggattatgaa tatgatgtcc actgtatcct taacaatatg cgctttatct cttggaggta
    10741 ttttagaaaa aacaggtatg cttgaagtag tagcatcttc tttactaaga ttagcaaagg
    10801 gtgtatttgg aacagtatta tgtactatgg taacttgtac aattaccaat ataatagcag
    10861 gtgaacaata tctatctata gttataccag gaagaatgta taataaggaa tataaaaaga
    10921 gaggtataca tcctaagatg ttgtcgagag ctttagaaga tagtggaact ttaacctctc
    10981 cattagtacc atggaataca tgcggggcgt atataacagc aacattaggt gtatctgcac
    11041 taacatatgg tccatacgct ttattaaata ttataaatcc aatagtatct ttagtattaa
    11101 ttgcatgtaa gtttaaaata gcaagaatag aagatgaacc tgaaacttgt ttaaattttg
    11161 atgtttgata atagataact tatgtaagaa cgtaggaact aggggggtag cctgtgggga
    11221 attagtaaga catattttag tgtgcgaaat tctataaatc agtaatcttg tgactttagc
    11281 catgggaggt tcagattagt aaatttatag ttttatatta gtctatattt tgatctttta
    11341 attgtttgta taaatttttt atagaaaaag atttctttta gacttgccta aaaattaggc
    11401 aagtctttta agtatgttct gattataaga gcactgtgtg acaagaatga tgagattatt
    11461 tttgctatac tttcgatata agaaatggaa ggaggttatg ttttatattc atatgaaata
    11521 attagttaca cagtaaattt tcgtataatt tggtataaaa agattagaaa tgaaaataaa
    11581 gtaatggagg aataaaatga ctagaatatc caatataaca atagctgaac aatctgagta
    11641 ctgttttttg gcaatacgaa aaacaattga ctttatggta gagttttcag agttttcaaa
    11701 acaaagtttt gaaaaaattt ctaaatatct ggaggagaga ggaattttat cttcaggagc
    11761 accgattgta tgttttcata acatggattt agcaaaattg gatgttgaag tagggtttcc
    11821 tgtagccact tatatagatg ggaaaaatga gattctacaa aggattgttc ctgcacagaa
    11881 aataataact gcaattgatt taggacctta tgaattacaa gaccctactt tagaagactt
    11941 attttcatgg gcaaatagca atggatataa acttcatggc gatatatatt accagtattt
    12001 aaatgatatc aatcgtccag ctagtgaata tcttacgaaa atgatgttac ccattatata
    12061 gaaagttaaa gattatcgtt tgttagttga taattagaag aacatttact gctactacat
    12121 aaattttatc ctaaataata tcaaggtgat ttaatttatg tattctaatg taaccccaat
    12181 atattgttaa ggtctactta agatgctcaa aaggctatgc ttaatttatt gttatataat
    12241 ttgtagagtg gtataattta gaggtaatat tatatagata tataggggga aaatatgaaa
    12301 atatatgata tattaacacg tttttatatt caagatatag aaaaagccat acctttttat
    12361 gaaaatcttt taaaagaaaa atgttctttg cgtttttcct ataaagaagt tgggttggaa
    12421 ttagcgcaaa taggaaatgt tttactatta tctggttctg atgatgctct taaaccattt
    12481 atagaaacaa aatctacttt tatggttgat tctgttgatg aatggagaag ttatctattg
    12541 gataatggag cagttgtagt aagagataag aaaaaagttc ctacaggata taatatgaca
    12601 ttgagacatc cagatggtac aattatagaa tatgttcaac atacaaagca ggattagtta
    12661 aaaacagatt ttactactga gcaactaatt agaatctatt cttagacgta caatttatta
    12721 tttttggtat taacagatta taagtcaaat tataatattt caaaatagaa tagaaattta
    12781 tttacataaa agagagtgtt taaaaatgaa ctttttagtt catgaagcac tcttttttgt
    12841 atgaaatcaa atacatcaat atataaaata aactgatgaa gagcttaaat taaaataaaa
    12901 agattcataa caggacccca ttaaacttta agatagggta agtttgtaat catattaaat
    12961 aattgtaaaa agctgttttt agtcgtataa tataaatatt tatcatttaa ttaaggaata
    13021 taaaaggaat ctaataagaa gaaaataata aataactttt tagatttact aaatgaaaaa
    13081 ttattgaaaa ttaaaggatt agagtcttta atcagaaagt aaaaagtaat atattaaatt
    13141 tatgaattga aaacttaaac aacaaatttg tttataaaat agcaagattc attaaatata
    13201 actaagtaat aattgatata atgttgcatg gataaaagga gggaagctgc aatgtcttac
    13261 aaagtaatta atgtcataac tgctattgac tatatagagg agcatttatc agaaaagctg
    13321 gatttagata tagttgctaa tgctgttcat tactcaaaat atcacctgca tagaactttc
    13381 acaacttctg ttggcctaac aatgcatgat tacataaagc ggagaaagtt gacagaagca
    13441 gcaaaattat tggcattttc aaagaaacca attattgaaa ttgcattgat agctggatat
    13501 gaaagtcaac aggcattcac ttctatattt aaagctatgt ataaaaaatc tccaaataaa
    13561 tataggaaag aacaagaatt ttatcctttg cagctaagat ttgttttgaa aaaagataac
    13621 ctattttcaa agaatgtact agagttggaa aaagaaatta aacttgcaag tatgtctgac
    13681 ataccattat ggatgaattt agtgagatta gtcatagatg gatttccaaa tttacaagag
    13741 gaagaatata tatatcaatt agaacagtat attttagaag aaagagcttt aatattaaaa
    13801 cttaataatg tcgctattgc aaatatggtg tttaatagag aaactagaag tattgatttt
    13861 tttggaatac atccacagta tcgtaattca gacattgcac aagtgttttt gaagaaagtt
    13921 attgaagatt tcttgataga tacagatatt tcaattacta cctttcgaga gggtgacaaa
    13981 gctgatacag gacatagaga tatgataaag aagttgggct ttgcagaagc agagttgctt
    14041 gtggaatttg gatatcctac tcaaaaattt attcttcctt taagttaaat agcaagaatg
    14101 attaaaaact ccaaaacaca attaagtata atgattcagt gtaagtactt aattaacaaa
    14161 ggaggattta aatgtctgaa aataaacaag tagtaaattg cgatattatt gcatctaatt
    14221 ctaaacataa tatgtgtgaa tatgatgcag agttattaac tttttttcca gatgagattg
    14281 cacatctaat agagataaat aacaagctag acaatgcttt taagaaagct gaaaatcttg
    14341 ttgacaagct tgataaggat tatatggacg ctaaaatgta tatggtaaaa aatcgtggtg
    14401 aaatagaccc acatgaaatg tttcaaaatg aacaaggact aaaacaaata gataactatg
    14461 gggcttttat ggtaaaagtt cgagataaga ttaataaaat caaagattct ccatactttg
    14521 ctagaataga ttttaggctg aaagatatgg atgatgaatc taagtattat attggtagat
    14581 ttgcatttga ttatgaagat gagcttataa tacttgactg gcgttctcca attgccagta
    14641 tgttttatga ttatgaaatc ggaaaagctg gatatgatgc tccaatagga tgggtggatg
    14701 gagaaataac aagaaaacga caatttaaaa ttaaaaatgg aaaattagaa tatgcactag
    14761 aaagttccat taatatacaa gatgatattc ttcaaaaaga acttagtcac acttcagatg
    14821 aaaaaatgaa gtcaatcatt tcaacaatac aaaaggaaca aaatcaaatt attagaaatg
    14881 ataaagctga tacactgatt attcaagggg tagctggttc tgggaaaaca tctatagcat
    14941 tacacagaat tgcttttctt ctttatcgat ttaaagataa aatatcagca aataatgtaa
    15001 ttatcctatc acctaataaa gtctttggag actatatttc caatgtcctt cctgagcttg
    15061 gagaagagcc attatgtgaa ttaagttttg aaaatatagc agaggtacaa ttagatagag
    15121 ttattaattt tgaaagtgag aaagacccat tagaaataaa tgatgctaaa tggtctgaaa
    15181 gagtgcgttt taaatctaca cttgattttg taaaacttat tgatgattat ataaaacaaa
    15241 tgccaaataa gatttttatt ccaaaagact acacttttgg aagttttaca gctaaaagtg
    15301 actggataca gagtagattt gaggcatata acagatatcc agttaagaag agattagaga
    15361 aggtagctga agacattcat tataaatttg aatcagataa tattatggaa gaagatttac
    15421 caaaggtaaa aagtatactt aaaagtttga atggaatgtt gacaataaaa aatactctta
    15481 cactatataa ggatttcttt aaacaaatga atatatcaaa catgtttgtg atggcaacaa
    15541 aaaaaactct tgaatggtca gatgtatacc catttatata tattcatgct gcatatgaag
    15601 gtatacaaga agataagatt ataagacatg ttgttattga tgaaatgcag gattacacac
    15661 ctattcaata tgctgtaata aacttacttt ttaagtgtaa aaagactatt cttggagatt
    15721 ttggacaatt ggttaatcct aatcacactc atacactaga tgatatgaaa cagctatata
    15781 atgatgggga actagttatg ttgaataaga gttaccgttc tacttttgag attattaatt
    15841 tcgccaaaaa gattcaagac atatcttcat tagagcctat tgaacgacat ggtgaagaac
    15901 cagctttatt aaaatgtaat aacgagcaag atgaaataaa taagataaaa atagaaattg
    15961 aagaatttaa aaagagtgat aatgcgactc ttggtattat attaaagaca gataatgacg
    16021 ctaaagctgt atatgatgcg ttaagagaaa aatatagtgt tcacttaata tcttctgaaa
    16081 gttctagctt tacaaaagga gtatccataa cttctattaa aatgtcaaaa ggtttagagt
    16141 ttgatgaagt tattgtacct tcagtaaata ataaaacata ttatagtgat tatgaccgca
    16201 gtttgttata tattgcttgt acacgagcta tgcataaact taagttgaca tatactggtg
    16261 aattgactca
//
LOCUS       DAELLV010000070        16009 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.070, whole genome shotgun sequence.
ACCESSION   DAELLV010000070 DAELLV010000000
VERSION     DAELLV010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16009
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.070"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            157..1605
                     /locus_tag="KPD58_001786"
     CDS             157..1605
                     /locus_tag="KPD58_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBG0265556.1"
                     /translation="MKLGKDFLWGGAISANQSEGFYLDGGKGLSQFDKLPLNDKRLKD
                     VCLDEDNILNENNQYYPSHIGIKFCENYKEDIAMLAEIGFKCFRFSISWSRIFPTGEE
                     SEPNEKGLQLYDNIIKELKKYNIEPLITISHFDIPYSLVEKYSGWADRRVVNLYVKYA
                     EILMKRYKDDVKYWIPFNEMNMIMHIPYIGAGLTFIADENQLQKKYQAAHHQLLANAK
                     TIEIGRYINKEFKFGCMLAAGKTYPYTCKPEDVFATLEHERYNLFFSDVQVRGVYPSF
                     MNYYLKSNQIKLIIKEEDLSTLKDNTVDFVSFSYYSSACSSARAEGLEKSKANGPETI
                     KNPYLPKSNSVWQNDPVGLRITMNQLYERYQLPLFIVENGLGTQDIVNEDGKIIDDYR
                     IDYMKEHIKNMILGVNEDGIELIGYLMWGCIDVTSVSEGKMTKRYGIIHVDSDDYGRG
                     SFKRTKKQSAFWYKKVIETDGEILFNDMETHA"
     gene            1631..4021
                     /locus_tag="KPD58_001787"
     CDS             1631..4021
                     /locus_tag="KPD58_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBG0265557.1"
                     /translation="MSLIFTTKDQLIEQGFSKDTIKSNETLFTLANGHLGVRGNIEES
                     DFNNEYVDNMGTYVNGFYEESPITYGESAYGYAKQNQTICKLPNAHIVNFSIEGDYFD
                     LSKGITSEHSRILDLNEGILKRSFIWENSCGRKVKVSIERLVSQDIEELMMISYTITP
                     LNFNGKIKFKNKMENSMEKFNCEKVDDPRVASINKKQFKINVKKYNNKYFMNLVTERS
                     RLGLWCGFDSYITCGNITNETYNVSDECIEREIEVYAKKDERLNLEIVVGYSRVYSDL
                     NLNEERLKSLECLESLKCLESEAHLKSLELVLREACNIGFDKVKNIQRKKMNEFWTTS
                     DVEIEGDKTLQLGIKTNLFHIYQSAGRDGKTNISAKGLTGDGYEGHYFWDTEMYILPF
                     FIYTQPSIARALLDYRYSILDASRERARELGINKGCLYSWRTINGNECSAYYPTGTAQ
                     FHINADIAYGIRTYFEATNDEEFMMQNGLEILIETARFWVEFGDYIESKNNKFCINGV
                     TGPDEYTAIVNNNYYTNLMAKYNLIYAVQMVDKFKHNLFSNEVFRKVKLQEYEVKNWI
                     NAAQNMYLPYDEEKKISKQDDSFLEKSVWDFKNTPKEKYPLLLHYHPLTIYRYQVNKQ
                     ADTVLSALLFPDEFSLEQKKRDFEYYEKITTHDSSLSRSIFGMMASEIGNHEKAYNYF
                     MDTALMDINNLQKNTSDGIHAANMGGTWMSLVYGFAGMKIKNDKLSFEPRIPKHWKNI
                     KFNILFRDNLVSVNIEEYKSYYKLIKGRNITILHDNKEIKLSKNIIEVNNCFKGSN"
     gene            4023..4694
                     /gene="pgmB"
                     /locus_tag="KPD58_001788"
     CDS             4023..4694
                     /gene="pgmB"
                     /locus_tag="KPD58_001788"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBG0265558.1"
                     /translation="MKAVLFDLDGVITDTAKYHFEAWHEIAKSLDIEIDETFNEKLKG
                     ISREESLDKILLSANKVDSYTKEEKLKVCEEKNNIYIKLINSLTYKDILPGINELIKE
                     LKKNNVKLGIASASKNAPRILESLGIKDEFDCIVNPSLLKRGKPYPDIFIEGSKILNL
                     KQYDCVGIEDSYSGIKAINAANMTSIGVGKKEVLGKCDHIVEDTSLLNCNTLLQAWSK
                     KINLD"
     gene            4733..6097
                     /locus_tag="KPD58_001789"
     CDS             4733..6097
                     /locus_tag="KPD58_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG0265559.1"
                     /translation="MKNKYEEVAQLIIDGLGGKDNIIDLTHCMTRLRFILKDEKKVNA
                     DKLKSIDKVVGVNSTSTQYQVIIGNEVGVVFNAIINKGINTTGDIKSDIKNKKNSILN
                     NIIDTITGCMTPMIPALTASGMIKVILSLCTTFNWLSSNSSTYRVLDIIGDVAFYFMP
                     IILAINAAKKFNVNTSIAVIVVGVFLHPNFSAWVSSGDPISFIGVPIQGVIYVASVIP
                     ALLTVWMMSYIEKFIDKLTPSMLKTILNPTLVLLISAPIALIVIGPIGNFLGEGLASI
                     INLLQGRLGFIMVCLLAAAMPFIVMTGMHHALTPIFISAFATTGQESLILISQVCANL
                     AQGGATLAVALRSKDSKMKQLASAAWISAIMGITEPALYGVTLKLKKPAMLASIGAGI
                     AGLFAGITHVTLYVPQNSIMAVLGLSGEKGISNIVNGLIVIFIALVAPFIMTYVFGVE
                     KNVK"
     gene            6444..7286
                     /locus_tag="KPD58_001790"
     CDS             6444..7286
                     /locus_tag="KPD58_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG0265560.1"
                     /translation="MIINKILNNNVVITLDDNDEEVIVMGKGIGYQKSKGNLIDKTKV
                     NKVFRISNKEISNKLQELLNNIPIEHMKLSSEIIEYAQIKLNKKLNESIYISLSDHTY
                     SAIQRMKEGINVKNAILWEIKRFYKEEFEIGMKALDIIENKTGIKLPEDEAGFIAFHI
                     VNAQLSEGHTLASDITKLIQEVLSIVRYHFRIEFHEESVFYYRFIMHLKFFAQRLLLD
                     SAHEGETDKELLSIIKSKYNKEFECVVKIKNFIKKQYNYILTDDELIYLTIHLAKVVK
                     DSNM"
     gene            7431..9359
                     /locus_tag="KPD58_001791"
     CDS             7431..9359
                     /locus_tag="KPD58_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG0265561.1"
                     /translation="MGKYKQLAQEIVNNVGGKENISGLVHCITRLRFTLKDESIANDN
                     VLKNMEGIVTVMKSGGQYQVVIGNHVEAVYKDVVEIIGLDDSSTSSETKKSGNILDKG
                     IDIISGIFQPVLGIMAACGMLKGFNALFVAMGLYSDVSGGYMVINAAGDALFTFLPLF
                     LGYTSAKKFGLKPMLGLALGAAVCYPAIQGSSISAGADVMYTLFKGTMFASPVYIDFF
                     GLPIVSIDYTGTVVPIIFIVYFASRCEKLFNKCVPNLVKFFFVPMLTLLITVPVALIV
                     IGPIATFGSTLISQIVISIRDFSPLLAGAIVGFTWQILVIFGMHWGFIPVYINNVMTL
                     GYDNVMMPFFACTFASSAVVLAIFFKTKDKKLKEMAIPNFISGIFGVTEPAIYGILLP
                     LKKPFIISCIASGIGGAFYGFFNLRKFITGGMGIFELPAMIEPDGGMGNLIVALSGIA
                     ISMVVAFVLTMILYKDEKVEESKKVRNKGKEEIKEVKSTKLEREIVTSPIKGEVLKLS
                     DIEDAAFASGVLGQGVAIIPSDGKVVAPVDGVVTTLFPSLHAIGILSDTGVEVLIHIG
                     LNTIQLEGRGFEACIKQGDRITKGQTILNFDVYAIKELGYSTVTPIVITNSSQFLDVV
                     ETESKNIELEDNLITVLF"
     gene            9420..10847
                     /gene="ascB"
                     /locus_tag="KPD58_001792"
     CDS             9420..10847
                     /gene="ascB"
                     /locus_tag="KPD58_001792"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBG0265562.1"
                     /translation="MSFKNDFLWGGATAANQCEGGYNEDERGLANVDVCPTGKDRTAV
                     ITGKLKMFDFDDEHYYPAKTGIDMYHNYKEDIKLFAEMGFKVYRMSIAWSRIFPKGDE
                     ETPNEKGLQFYENIFKECKKYKIEPLVTITHFDCPMHLVKKYGAWRNRKMIGFYEKLC
                     NVIFRRYKGLVKYWLTFNEINMILHAPFMGAGICFEEGEDVEKIKYQAAHHELVASAI
                     ATKIAHEVDSNNLIGCMFAAGSVYPYSCNPNDVWEATKLDRENYFFVDVQSKGKYPNY
                     ALKYMEQKGITPEMEPGDIELLNKYTVDFISFSYYNSRCVRTDENADDMAEGNIFTSA
                     KNPYLSYSQWGWPIDPLGLRITLNHVYDRYEKPLFIVENGLGAKDIADENGYVEDDYR
                     IDYLREHIKAMHDAVSIDGVDLLGYTTWAPIDLVSAGTGEMEKRYGFIYVDRDNSGNG
                     SLRRMKKKSFEWYKKVIASNGEDLY"
     gene            11294..12070
                     /locus_tag="KPD58_001793"
     CDS             11294..12070
                     /locus_tag="KPD58_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG0265563.1"
                     /translation="MKYQHIIEVDKELCIGCGLCKNDCPVNNIIIENKKSVIKKQDCL
                     MCGHCAAICPTKAITLTGFDEPPIELTNKPKLDSDELLMAIKSRRSIRKFKDKEVSSE
                     IIKQIIEAGRYTPSAKNSQDVSYIVLDNKKSIYENEAVKFFRKIKPIANIAIKYSKEV
                     EIDDNFFFKHAPIAIMIITKDKISGSLAASNMALMAESYGLGVLFSGYFSDVANNSPK
                     LKKLLGLKRSNHVLTTLVIGYPDVKYRRTAQKEVATVRYL"
     gene            12086..12451
                     /locus_tag="KPD58_001794"
     CDS             12086..12451
                     /locus_tag="KPD58_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG0265564.1"
                     /translation="MEKECQQRMQEITSKFKECRKAISAMGDETRQLILIALLESDFN
                     GIRVGEITKKTHLSRPAVSHHLKILKEAEIVNLRREGTKNYYYLDSKKSQWKSLTELI
                     NLIFVGIQHISENDIRKGD"
     gene            complement(12617..13366)
                     /locus_tag="KPD58_001795"
     CDS             complement(12617..13366)
                     /locus_tag="KPD58_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440038.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBG0265565.1"
                     /translation="MILYFSGTGNSRYVARKIAQELNDELISLNQLIKEEKTDELISA
                     DKPFIFVCPTYAWRLPIVVTDFIKETKFLGSNRVYFVMTCGGDTAKSINYIQKLCKYK
                     EWQLKGMAEIKMPENYIALFSTTDKETSKQMIKEADKQIYRIISDIRNENEFETITPS
                     GLGGTIKSGIINTVFYKTIISAKGFHYTDKCIGCGKCVELCPLNNINLKNKKPVWKNN
                     CTHCMACICGCPTEAIEYKNKTQNRERYYLE"
     gene            13544..13696
                     /locus_tag="KPD58_001796"
     CDS             13544..13696
                     /locus_tag="KPD58_001796"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265566.1"
                     /translation="MRRDNNLLMKDYKYKGYFKGVEIFDNVNDLNLNTLTTVSRFVLV
                     FLFTIT"
     gene            13824..14249
                     /locus_tag="KPD58_001797"
     CDS             13824..14249
                     /locus_tag="KPD58_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0265567.1"
                     /translation="MINENLKSLRKINQYTQEELAEKLNVSRQSIAKWESGESIPDIC
                     SCIKLAKLYNVKLDDLVNHSEEKTGIIVPPKGKFFFGAVVVGERGQIVIPKEAREVFN
                     INAGDKLLVLGDEERGLGIVHQRDLINFIGHVGVVRKEE"
     gene            14281..15468
                     /locus_tag="KPD58_001798"
     CDS             14281..15468
                     /locus_tag="KPD58_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HBG0265568.1"
                     /translation="MSKIVFFSIPAYGHTNPTIEVVRELVDRGNEVLYYSFNEFKDKI
                     EGAGAKFICCDKYLPELLPGDEKKIGKDFPGLIEMIVDTTISLDEKVCRELKDFNPDC
                     IVSDSLSFWGKLFAQKLNIPYVCSTTTFAFNKHTAKLMKQNFIEIIRMIFGVRRINKK
                     IKLLQRKGYEVKNFISIVSNDNDTDTIVYTSKEFQPMVETFSSKYSFVGPSVSKLIIE
                     PKERKRKLIYISLGTINNKNVSFYKNCISAFKDSDVDVIMSVGRSTDIKSLGNIPNNF
                     EVKNSVEQIAILQKTDVFVTHSGMNSVNESLYYGVPMVLFPQHSEQRMVAKRVVHLGA
                     GIMLKEDKSESIKKAVFQVINDNEYKENATKLSKSFYNAGGSKKAADVILQIICNSRQ
                     VDI"
     gene            15660..>16009
                     /locus_tag="KPD58_001799"
     CDS             15660..>16009
                     /locus_tag="KPD58_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HBG0265569.1"
                     /translation="MKKYKVIFIVLIIGIISVTGFVVYKNCIKPWKDAETYIDKYMVK
                     QDISKDNIKSITKEKAKKASYEGILYKVYYKDDPRYEYQYFYSDNYYALYVNKVMLQI
                     YDMENNNKLLRSNE"
BASE COUNT         6288 a         1682 c         2848 g         5191 t
ORIGIN      
        1 tgaaagtatt gaaaatagac aatattaatt atagtgagta aaaaatataa ttggattttt
       61 aaattgaatt atttaaaagg tatagtatga aaaaaatctt ataactaaat atgagataga
      121 aattataaaa ctttatattg tgaaagaggt agatgcatga aattagggaa agatttttta
      181 tggggagggg caatttcagc caatcaaagt gaaggatttt atttagatgg aggaaaaggt
      241 ttatctcagt ttgataaact cccattaaat gataagagat taaaagatgt atgtttagat
      301 gaggataata ttttaaatga aaataatcaa tactatccaa gtcatatagg gattaaattt
      361 tgtgaaaatt acaaagaaga tatagcaatg ttagcagaaa taggttttaa atgttttaga
      421 ttttctatat cttggtcaag aatattccca acaggagaag aaagtgaacc aaatgaaaag
      481 ggattacaac tttatgataa tataataaaa gaattaaaaa aatataatat agagccatta
      541 ataacgataa gccactttga tataccttat tctctagttg aaaaatattc tgggtgggct
      601 gatagaagag tggttaattt atatgtaaaa tatgctgaaa ttttgatgaa aagatataaa
      661 gatgatgtca aatactggat tccttttaac gaaatgaata tgataatgca tattccatat
      721 ataggtgctg ggttaacttt tatagctgat gaaaatcaac ttcagaaaaa atatcaagca
      781 gcacaccatc aattattagc taatgcaaaa actatagaaa taggaaggta tataaataaa
      841 gaatttaagt ttgggtgtat gttagctgct ggaaaaacat atccatacac atgtaaacca
      901 gaagatgtat ttgcaacttt agagcatgaa aggtataatt tgtttttttc tgatgttcaa
      961 gttaggggtg tgtatccaag ctttatgaat tattatttaa aatcaaatca aataaaatta
     1021 ataataaaag aggaagattt atcaacactt aaggataata ctgtagattt tgtatctttt
     1081 agctattatt ctagtgcttg tagttcagct agagctgaag gtttagaaaa atcaaaggca
     1141 aacggaccag aaaccataaa aaatccatat cttccaaaat caaatagtgt atggcaaaac
     1201 gaccctgtag ggttaagaat aactatgaat cagttatatg aaaggtatca gttaccttta
     1261 tttatagtag aaaatggact gggtactcaa gatattgtga atgaagatgg aaaaataatt
     1321 gatgattatc gtatagatta catgaaagaa catattaaaa atatgattct tggagtaaat
     1381 gaagatggaa tagagcttat aggatatctt atgtggggat gtatagatgt aaccagtgta
     1441 agtgaaggaa agatgacaaa acgatatggc ataattcatg ttgattctga tgactatgga
     1501 agaggctcat ttaaaagaac taaaaaacaa agtgcttttt ggtataaaaa agtaatagaa
     1561 acagatggag aaattttatt taatgacatg gaaactcatg cataattcat tttaagaaag
     1621 gagtgttatt atgagtttaa tatttactac taaggaccaa cttatagagc aagggtttag
     1681 taaggataca ataaaatcca atgaaacgct gtttacacta gctaatggac atttaggagt
     1741 tagagggaat attgaagagt ctgattttaa taatgagtat gtagataata tgggtacata
     1801 tgtaaatgga ttttatgaag aatctccaat tacatatgga gaaagcgctt atggctatgc
     1861 aaaacaaaat cagactatct gtaagttacc taatgctcat atagtaaatt tctctattga
     1921 aggagattat tttgatttat caaaaggtat tacaagtgaa cattctagga tactagattt
     1981 aaatgaaggg attcttaaac gttcatttat atgggaaaac tcttgtggta gaaaagttaa
     2041 agtatctata gaaagattag tatcacaaga tatagaagaa ttaatgatga tttcatatac
     2101 aataacacca ttgaatttta atggaaaaat aaaatttaaa aataaaatgg aaaatagtat
     2161 ggagaagttt aattgtgaaa aagtagatga cccaagagtg gcatctataa ataagaagca
     2221 atttaaaatt aatgtaaaaa aatataacaa taagtatttt atgaatttag ttacagaaag
     2281 aagtagatta gggttatggt gtgggtttga ttcatatatt acttgtggaa atattacaaa
     2341 tgagacctat aatgtttcag atgaatgtat tgagcgagaa atagaagtct atgccaaaaa
     2401 agatgaaagg ttaaatttag aaattgttgt aggctatagt agagtatatt ctgatttaaa
     2461 tctaaatgaa gaacgtttaa aatcattaga atgtctggag tcgttaaaat gtttagaatc
     2521 agaagcacat ttaaaatcat tagaattggt tttaagagaa gcatgtaata ttggatttga
     2581 taaagtcaaa aatattcaga gaaagaaaat gaatgagttt tggacaacct ctgatgtaga
     2641 gattgaaggc gataaaactc ttcaactggg gataaaaacc aatttatttc atatttatca
     2701 atcagcagga agagatggaa agactaatat atcagcaaaa ggtcttacag gtgatggcta
     2761 tgaggggcat tatttctggg atacagaaat gtatatatta ccatttttta tatatactca
     2821 accatccatt gctagagctt tattagatta tagatactca atcttagatg catcaagaga
     2881 aagagctaga gaacttggaa taaataaagg ttgcttatat tcatggagaa ctataaatgg
     2941 taatgaatgc agtgcatatt atccaacagg aacagcacag tttcatataa atgcagatat
     3001 agcttatggt attagaacgt attttgaagc aactaatgat gaagaattta tgatgcaaaa
     3061 tggcttagaa attcttatag aaacagcaag attttgggtt gaatttggag attatataga
     3121 atcaaaaaat aataaattct gtataaatgg agtaacagga ccagatgaat atacagcaat
     3181 agtaaacaat aattactaca ctaatttaat ggctaagtat aatttaatat atgcagtaca
     3241 gatggtagat aaatttaagc ataatttatt ttcaaatgaa gtatttagaa aagttaaatt
     3301 acaagaatat gaagtgaaaa actggataaa tgccgctcaa aatatgtatt taccatatga
     3361 tgaagagaag aaaataagca aacaagacga ttcattttta gaaaaatcag tatgggattt
     3421 taaaaataca ccaaaagaaa aatatccatt acttctccat tatcatccat taacaatata
     3481 tagatatcaa gtaaataaac aagcagatac tgtactttca gcgttgcttt tccctgatga
     3541 gtttagctta gaacaaaaga agagagattt tgaatactat gaaaaaataa ctactcatga
     3601 ttcatctttg tcaaggtcaa tatttggaat gatggcaagt gaaattggaa atcatgaaaa
     3661 agcttataac tattttatgg acacagcatt aatggatatt aataacctac aaaaaaatac
     3721 aagtgatgga atacatgctg ctaatatggg tggaacatgg atgagtttag tatatggatt
     3781 tgcaggaatg aaaataaaaa atgataaatt aagttttgaa ccaaggatac ctaaacactg
     3841 gaaaaatatt aaatttaata tattatttag agataattta gtttctgtaa atatagaaga
     3901 atataaatct tattataagt taatcaaagg tagaaatata accatattac atgataataa
     3961 agaaataaaa ttatcaaaga acataataga agttaataat tgttttaaag ggagtaatta
     4021 atatgaaagc agtcttattt gatcttgatg gagttataac agatactgct aagtatcatt
     4081 ttgaagcttg gcatgagata gcaaaatcgc tagatataga aatagatgaa acttttaatg
     4141 aaaaattaaa aggcataagc agggaagaat cattagataa aatattgtta tctgctaata
     4201 aagtagatag ttatactaaa gaggaaaagt taaaggtgtg tgaagaaaaa aataacattt
     4261 atataaagtt aataaactca ttaacatata aagatatatt accagggata aatgagctta
     4321 taaaagaact taaaaaaaat aatgtgaaat tgggaatagc atctgctagt aagaatgcac
     4381 ctaggatatt agaatctttg ggaataaaag atgaatttga ttgtatagta aatccatctt
     4441 tattaaaaag gggaaaacca tatcctgata tttttattga agggagcaag attctaaatt
     4501 taaaacaata tgattgtgta ggaatagaag attcttattc aggtattaaa gctataaatg
     4561 ctgctaatat gacatcaatt ggtgtgggga aaaaagaagt actaggaaaa tgtgaccata
     4621 ttgtagaaga tactagctta ttaaattgca atacactatt acaagcttgg agtaaaaaga
     4681 ttaatttgga ttaaaattat attaaaaatt attttaaaag agaggataag gtatgaagaa
     4741 taaatatgaa gaagtagctc aattgataat tgatggcctt ggaggaaagg ataatataat
     4801 tgacttaact cattgtatga cacgattaag gttcatctta aaagatgaaa agaaagtcaa
     4861 tgcagataaa ttaaaatcta tagataaggt agttggagta aactcaactt caactcaata
     4921 tcaggttata ataggtaatg aggtaggtgt tgtatttaat gcaataataa ataaggggat
     4981 aaataccaca ggagatatta aaagcgatat aaaaaataaa aaaaatagta ttttaaataa
     5041 tataatagat acaataactg gttgtatgac acctatgata ccagccctta cagcatctgg
     5101 gatgataaaa gttatattat ctctttgcac cacttttaat tggctatcat caaattcaag
     5161 tacatataga gtattagata taataggaga tgtagcattt tactttatgc ctataatact
     5221 tgcaataaat gcagctaaaa aatttaatgt taatacatca atagcagtta tagtagtagg
     5281 tgtgttttta catccaaatt tctctgcatg ggtatcaagt ggggacccta tatcatttat
     5341 aggagtacca attcaaggtg ttatatatgt agcatctgtt atccctgcac ttttaacagt
     5401 atggatgatg tcatatatag aaaaattcat tgataaatta acacctagca tgttaaaaac
     5461 aattttaaat ccaactttag tattattaat aagtgcgcca atagcattaa tagtaattgg
     5521 accaatagga aatttcttag gagagggatt agctagtata ataaatttac tacaaggaag
     5581 gttaggattt ataatggttt gtttattagc agctgcaatg ccatttatcg ttatgacagg
     5641 gatgcatcat gcattgactc ctatattcat atcagcattt gcaactacag gacaagaatc
     5701 gctaatatta atatctcaag tttgtgctaa tttagcgcaa ggtggcgcaa cattagctgt
     5761 agcattaaga agcaaagata gtaagatgaa acagttagca tcagctgcat ggatatcagc
     5821 tatcatgggt ataactgaac cagctcttta tggagttaca ttgaaactta aaaaacctgc
     5881 tatgttagca tctataggag caggaatcgc agggttattt gcaggaatta ctcatgtgac
     5941 tttatatgtt ccacaaaata gtataatggc agtacttgga ttatcagggg aaaaggggat
     6001 ttcaaatata gtaaatggac ttatagttat atttatagca ttggttgctc catttattat
     6061 gacatatgtt tttggagttg aaaaaaatgt taaataaagt acagataatg tagtagagga
     6121 agtataatat taactattta cattaataga atttttatta ttattgacta taacttgttt
     6181 tgtatttata ttgatgcaaa gaaataagtt aatattaata agaataatta ttagaactga
     6241 caaaaagcaa cataaaagat tgatgaagta tatggaaata aaaagttgac aaaatatgtc
     6301 tgatgtttta tcatattaat tggaattagg tagtaattgt ttaagtacaa gctaagctta
     6361 ttcattaata atagcagtat cctaggaagg agtatgccca ttattatgga taagcttttt
     6421 ttatgcttaa aaggaggata atcatgataa ttaataagat attaaacaat aatgttgtaa
     6481 tcacattaga tgataatgat gaagaagtta tcgttatggg taaaggaatt ggttatcaaa
     6541 aatcaaaagg caatctaata gataaaacta aagtaaataa agtatttaga atatcaaata
     6601 aagaaatatc aaacaaacta caagaattac tcaataatat tcctatagaa cacatgaaat
     6661 tatcaagtga aattatagaa tacgcacaaa taaagttaaa caagaagcta aatgaaagta
     6721 tttacatatc cttaagtgac catacatatt ctgcaattca aaggatgaaa gaaggaataa
     6781 atgtaaaaaa tgcaatcctt tgggaaatta aaagatttta taaagaagag tttgaaattg
     6841 gaatgaaagc actagatatt attgaaaaca aaacaggtat aaaattacca gaagatgagg
     6901 ctggatttat agcctttcat atagtgaatg ctcagttaag tgaaggtcat actttggcat
     6961 ctgatattac gaagttaata caagaagtac ttagcatagt taggtatcat tttaggatag
     7021 aatttcatga agaatcagtg ttttattata gatttataat gcatttaaag ttttttgcgc
     7081 aaagattgct tttagatagt gcacatgaag gtgagacaga caaagaatta ctaagcatta
     7141 ttaaatctaa atataataaa gagtttgaat gtgtagttaa aattaaaaat ttcattaaaa
     7201 aacaatataa ctacatatta acagatgatg agcttatata tttaacaata catttagcta
     7261 aagtagttaa agactcaaat atgtaagtga aataattttt cagtagtata ttaagaatga
     7321 ttgaaaggag tgatactaac aaaattatac ttatggttgg atggtaacat tgagttggcc
     7381 aaaccttcaa atttcaaaaa attaatttaa acgaaatatg gaggagaaaa atgggaaaat
     7441 acaaacaatt agcacaagag atagtaaaca atgttggtgg aaaagaaaat attagtgggt
     7501 tagtacactg tattacaaga ttacgtttta ctttaaaaga tgaaagtata gcaaatgaca
     7561 atgtattaaa aaacatggaa ggtattgtta ctgtaatgaa aagtggagga caatatcaag
     7621 tagttatagg aaatcatgtt gaagctgtct ataaagatgt agttgaaata attggtttag
     7681 atgattcaag tacaagttca gaaactaaaa aatcaggtaa tatactagat aaaggtatag
     7741 atataatatc aggaattttt cagccagtgt taggaataat ggcagcttgt ggtatgttga
     7801 aaggatttaa tgcattattt gtagcaatgg gattatatag tgatgtatct ggtggttata
     7861 tggtaataaa tgcagcagga gatgcattat ttacattcct tcctttgttc ttaggatata
     7921 cttcagctaa gaagtttgga ttaaaaccaa tgttgggatt agcactagga gctgcagtat
     7981 gttatcctgc tatacaaggt agcagtatat cagcaggagc agatgtgatg tacactttat
     8041 ttaaaggaac aatgtttgca tcaccagtat atattgattt ctttggacta ccaattgtat
     8101 caattgatta tactggaaca gtcgtaccaa ttatatttat agtgtatttt gcttctaggt
     8161 gtgaaaaatt atttaataag tgtgttccaa atttagttaa gtttttcttt gtgccaatgc
     8221 ttacattatt aataacagtg cctgttgcac ttatagttat aggccctata gcaacatttg
     8281 gttctacact tatttcacaa attgtaattt caataagaga ttttagtcca ctattggcag
     8341 gtgctatagt aggatttact tggcaaattc ttgttatttt tggtatgcac tggggattta
     8401 ttcctgtata tataaacaat gttatgactt taggatatga taatgttatg atgccattct
     8461 ttgcctgtac atttgcaagt tcggcagttg tgttggcaat atttttcaag actaaagata
     8521 aaaaactaaa agagatggca ataccaaact ttatatctgg tatatttgga gttacagagc
     8581 cagcaattta tggaatatta ttgcctttaa agaaaccatt tataataagt tgtattgcta
     8641 gtggtattgg aggcgctttc tatggattct ttaatcttag aaaatttata actggaggaa
     8701 tgggaatatt tgaacttcca gcaatgattg aaccagatgg gggaatgggt aacttaatag
     8761 tagctctttc tggtatagct atatcaatgg tcgtagcttt tgtattaact atgattttat
     8821 ataaagatga gaaagtagaa gagtctaaaa aagtaagaaa taagggtaaa gaagaaataa
     8881 aagaagtcaa gtctactaaa ttagaaagag aaatagtaac aagtcctata aaaggtgaag
     8941 tattaaaatt aagtgatata gaggatgcag catttgcatc tggtgtacta ggtcaagggg
     9001 tggcaataat acctagtgat ggaaaagtag ttgctccagt agatggggta gttacaacct
     9061 tatttcctag tctacatgct atagggattc tttctgatac aggtgttgaa gtgcttattc
     9121 atattggatt aaatactatt caattagagg gaagaggttt tgaggcatgt ataaaacaag
     9181 gtgatagaat tacaaaaggt caaactatat taaactttga tgtttatgca ataaaagagt
     9241 tggggtatag tactgtaact ccaattgtta ttacaaattc atcacaattt ttagatgtag
     9301 tagaaacaga aagtaaaaat attgaattag aagacaatct aattacagtc ttattttagt
     9361 aaagattgag gtattagcat aatctagtgc tttggttata attttaggag gctttgacta
     9421 tgagttttaa gaatgatttt ttatggggag gagctactgc ggccaatcaa tgtgaaggtg
     9481 gatacaatga agacgaaaga ggattggcaa atgtagatgt atgtccaaca ggaaaagata
     9541 gaacagcagt tattacaggg aaactaaaaa tgtttgattt tgatgatgag cattattatc
     9601 cagctaaaac tggtattgat atgtatcata attataaaga agatataaag ttatttgctg
     9661 aaatgggatt taaagtatat agaatgagca ttgcatggag tcgaatattt ccaaaaggtg
     9721 atgaagaaac accaaatgaa aagggacttc aattttatga aaatatattt aaggagtgta
     9781 aaaaatataa aatagagcct ttagtcacta ttacacactt tgattgtcca atgcatttag
     9841 ttaaaaagta tggtgcatgg cgtaatcgaa aaatgatagg tttctatgag aaattatgca
     9901 atgttatctt tagaagatat aaaggattag tgaagtactg gctaactttc aatgaaataa
     9961 atatgatttt acatgctcca tttatgggtg ctggtatttg ctttgaagaa ggtgaagatg
    10021 ttgaaaagat aaaatatcaa gctgcccatc atgagcttgt agctagtgca attgcaacta
    10081 aaatagcaca tgaagttgat tcaaataact taattggatg tatgtttgca gctggaagtg
    10141 tatatcctta tagttgtaat cctaatgatg tatgggaagc tacaaagtta gatagagaaa
    10201 attacttctt tgtggatgta caatctaaag gtaaatatcc taactatgcg ttaaagtata
    10261 tggagcaaaa aggtattaca ccagaaatgg aacctggtga tattgaatta ttgaataaat
    10321 atacagtaga ttttatttca ttttcatatt acaatagccg ttgtgttaga accgatgaaa
    10381 atgcagatga tatggctgaa ggaaacatat ttacatctgc taaaaatcca tatctttcat
    10441 acagtcaatg gggatggcca attgacccat taggtttgag aattacacta aatcatgtat
    10501 atgatagata tgaaaagcca ctatttattg tagaaaatgg attaggtgct aaagatatag
    10561 ctgatgaaaa tggttatgta gaagatgatt acagaattga ttatctaaga gaacatataa
    10621 aagctatgca tgatgcagtt tctatagatg gggtcgattt attaggttat actacatggg
    10681 cacctattga tttagtaagt gctggtacag gtgaaatgga aaagcgttat ggatttatat
    10741 atgtagatag agacaatagt ggtaatggaa gtctaagaag aatgaaaaag aaaagttttg
    10801 aatggtataa gaaagtaatt gcttcaaatg gtgaagattt atattagaca agaaatctat
    10861 gttaaataag aaatctatat tagacaagaa atctatgtta aataagaact ttgtattaaa
    10921 taagaagagt ataaaaatta ataataagta taatttttaa cttttttaac tatagaaaat
    10981 caatatagaa ttattttaca ttgtattgat gcaatagtat gattcacaaa taaaatttta
    11041 gaaaataaaa ttgttaagaa gtatgggaag taagtgcaat ttactttgta ataaaagatg
    11101 aaaaaggcaa gcttaggttt taagcttgtc ttttttatta tgtataaact atgatatttt
    11161 aaaattctat gaaaaattta tttaatagag aaattaaaaa tttaatggta atcaaattct
    11221 ttaccaatga atataaaaaa tgtctacgtg ttataatagt ttaaactaat aaacgaaagg
    11281 tagatgaatg aatatgaaat accaacatat aatagaagtt gacaaagaac tttgcattgg
    11341 atgtgggctt tgtaaaaatg attgtccagt aaacaatata attatagaaa ataaaaagtc
    11401 agtaattaaa aaacaagatt gtctaatgtg tggacattgt gcagcaatat gtcccacaaa
    11461 agctattacc ttgacaggat ttgatgaacc tcctatagaa cttacaaata agccaaagct
    11521 agattcagat gaattactaa tggcaattaa atctagaaga agtatacgta aatttaaaga
    11581 taaagaagtt tcttcagaaa ttataaagca aattattgag gcaggtaggt atacccctag
    11641 tgctaaaaat tcacaagatg tatcatatat tgttttagac aataaaaagt caatttatga
    11701 aaacgaagca gtgaaatttt ttagaaaaat aaaacctatt gcaaatatag ctattaaata
    11761 ttcaaaagaa gttgagattg acgataactt tttctttaag catgctccta ttgcaataat
    11821 gataattaca aaagataaga ttagtggttc acttgcagca tcaaatatgg cattaatggc
    11881 agagtcttat ggtctaggtg ttttatttag tggatatttt tcagatgtag caaataattc
    11941 gccaaaatta aaaaagcttt taggtctaaa acgtagtaat catgttctta caacattagt
    12001 aattggatac ccagatgtta aatatagacg tactgcacag aaagaagtag caactgttag
    12061 atacttataa gagagggtgg attttatgga aaaagaatgt caacaaagaa tgcaagaaat
    12121 aacgtctaaa tttaaagaat gtcgcaaagc tatctctgct atgggtgatg agactagaca
    12181 attgatttta attgctcttt tagagagtga ttttaatggt atacgagtgg gagaaatcac
    12241 aaaaaaaaca catctttctc gtcctgctgt atctcatcat cttaagatat taaaagaagc
    12301 agagattgtt aatttgcgta gagaaggaac gaaaaattat tattatcttg attcaaaaaa
    12361 atcacaatgg aagtcactaa cagagctaat aaatcttata tttgttggaa tacaacatat
    12421 tagtgaaaat gatataagaa aaggtgatta aattatactt gatttaatac aaaaagattg
    12481 atttaggaac taaaaaattc actttgagac actatctttt gaactatacc cattttagga
    12541 gacggttaaa atatgtaaaa ctgtataaaa caatgggtat agttcatttt ataatggttt
    12601 aaatattaag aatatattat tctaaatagt atctttctct gttttgagtt ttatttttat
    12661 actcaatagc ttcagtgggg caaccacaaa tacatgccat acaatgagta cagttatttt
    12721 tccaaactgg ttttttgttt tttaaattaa tattatttaa aggacatagc tctacacatt
    12781 ttccacagcc aatgcattta tcagtataat gaaatccttt ggcactaata attgtcttat
    12841 aaaaaactgt atttattata ccacttttaa ttgtacctcc taagccacta ggagtaatag
    12901 tttcaaattc attttcattg cgtatatcag aaataatcct atatatttgt ttatctgctt
    12961 ctttaatcat ttgttttgat gtttctttgt ctgttgtaga aaataaagct atataattct
    13021 ctggcatttt aatttctgcc atacctttta attgccattc cttgtattta catagttttt
    13081 gaatatagtt tatggatttt gctgtatctc caccacaagt cattacaaag taaactctgt
    13141 tgcttcctag aaattttgtt tcttttataa aatccgtaac tactattggc aatctccatg
    13201 cataagtagg acatacaaat atgaatggtt tatctgctga tattaattca tctgtttttt
    13261 cctcttttat aagttgattt agtgatatca gttcatcatt taattcttgt gcaattttac
    13321 gagccacata tcggctatta ccagttccgc taaaatataa aatcataata atatctccct
    13381 aactattttt tatttattat agtaatttat tgatatattc tattcaaatg tatgtaaaat
    13441 ttagtagtta agaaaattat aacacattag taatttttat acttattggt aaagaatttg
    13501 attaccatta aatttagcaa caagacatca actggacttg tatatgagaa gggataataa
    13561 tttgcttatg aaagattata aatataaagg ttattttaaa ggtgtagaaa tatttgataa
    13621 tgtaaatgac cttaacttaa atactttaac aacagttagt agatttgtgc ttgttttttt
    13681 gtttacgata acttaaggaa atatgttacc tgaataataa atttatataa taaaagtata
    13741 taaatatata tatgaatgtt aagattagtt gatagaatct aaataataaa tccaatatta
    13801 taaacatagg aggtatgaaa aatatgatta atgagaattt aaaaagtcta agaaaaatca
    13861 accagtatac acaagaagaa ttagctgaaa aacttaatgt ttctagacaa tcaatagcaa
    13921 aatgggaaag tggagaatct attcctgata tatgtagttg cattaaactt gcaaaattgt
    13981 acaatgtcaa actagatgac ttagttaatc attcagaaga aaaaacaggt attatagtac
    14041 ctcctaaagg aaagtttttc tttggagcag ttgtagttgg tgaacgtggt cagattgtca
    14101 ttccaaaaga agcaagagaa gtatttaata taaatgcagg agataaatta ttggtacttg
    14161 gagatgagga aagaggatta ggaattgttc atcaacgtga tttgataaat tttataggtc
    14221 atgtgggagt agtacgtaaa gaagaatgat ttaaaaaaat tataaaagga ggtatgaaat
    14281 atgagtaaaa tagtattttt tagcattcct gcatatggtc atactaatcc tactattgaa
    14341 gtagttcgtg aattagtgga tagaggaaat gaggtattgt attattcttt taatgaattt
    14401 aaagataaaa ttgaaggtgc tggagcaaaa tttatttgtt gtgataaata ccttccagaa
    14461 ttactccctg gagatgagaa aaaaattggt aaggattttc ctggattaat tgagatgata
    14521 gtagatacta ctatttcttt agatgaaaaa gtttgtagag aacttaaaga ttttaatcca
    14581 gattgcattg tatcagattc attaagtttt tggggaaagt tatttgcaca aaaacttaat
    14641 attccatatg tatgctctac aacaactttt gcatttaata aacatacagc aaagttgatg
    14701 aaacaaaact ttatagaaat tatacgtatg atttttggtg taagacgcat taataaaaaa
    14761 ataaagcttt tacaaagaaa agggtatgaa gtaaaaaatt ttatttctat agtttctaat
    14821 gataatgata cggataccat tgtatataca tcaaaagaat ttcaaccaat ggtagaaacc
    14881 ttctctagta agtattcatt tgtaggtcca tcagtatcaa agttgattat tgaacctaaa
    14941 gagagaaaaa gaaagctaat atatatttcg cttggtacaa ttaataataa aaatgttagc
    15001 ttttataaaa actgtataag tgcatttaaa gattctgatg ttgatgtgat tatgtctgtt
    15061 ggtagaagta ctgatattaa aagtttagga aatataccaa ataattttga ggtcaaaaat
    15121 agtgttgaac agattgctat acttcaaaaa actgatgtgt ttgttactca ttctgggatg
    15181 aatagtgtta atgaaagttt atattatgga gtacctatgg tattatttcc tcaacattcg
    15241 gagcagagaa tggtagctaa gagagttgtt catttgggag caggaattat gttaaaagag
    15301 gataagtcag aaagtattaa aaaagcagta tttcaagtta taaatgacaa tgaatataaa
    15361 gaaaatgcaa ctaaactatc aaaaagcttt tataatgcag gaggctcaaa aaaagcagca
    15421 gatgtaatat tacagattat atgtaatagt agacaagttg acatataata gatttgttaa
    15481 aaatatatat acatacatgt aatttaatca aagtactgac agtggggtct tttaacctac
    15541 cagttggggc atatatattg atgctaaata tgagggatgt tatattttaa taaaaaagat
    15601 aacatccttt ttttagtgca ttatatcgag ttttaaataa taaatgaagg aggatcaata
    15661 tgaaaaaata taaagttatt tttatagtat taattatagg tattataagt gtaacagggt
    15721 ttgttgtata taaaaactgc attaaaccat ggaaagatgc tgaaacatat attgataagt
    15781 atatggtaaa acaagatatt tcaaaagata atataaaaag tattacaaaa gaaaaagcca
    15841 agaaggcaag ttacgaagga atactataca aagtgtatta caaagatgac cctcgatatg
    15901 agtatcaata tttttatagt gataattatt atgctctata tgtaaataaa gtaatgttac
    15961 aaatttatga tatggaaaat aataataaac ttttaagaag taatgagga
//
LOCUS       DAELLV010000071        16000 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.071, whole genome shotgun sequence.
ACCESSION   DAELLV010000071 DAELLV010000000
VERSION     DAELLV010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16000
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.071"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            324..1541
                     /locus_tag="KPD58_001800"
     CDS             324..1541
                     /locus_tag="KPD58_001800"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HBG0265570.1"
                     /translation="MMNVTLTDVKEARETIKNIVKRTDLLESVKLSEKTGANVFYKCE
                     NLQKTGSFKLRGACNKIASLTDEEKANGVIASSAGNHAQGVALGAKMTGIKSTIVMPA
                     TAPLAKVSATKGYGAEVVLNGAVYDDAYAKAVEIQKETRATFLHPFNDKYVIAGQGTI
                     SLEIFEQLDNKVDTILCPVGGGGIISGVAVAAKALNPNVKIVGVQTANIPSMKESIKN
                     GKVTTAFNDTTIADGIAVKTPGDLTFEIINELVDEIVVVEETEIAESILFMMESQKIV
                     SEGAGAVCTAAILSGKYVPAKDENVVCIISGGNIDINTLYRIIGVALAKEGRRYSFST
                     IMEDKPGNFAELTRIISENGGNILSANQGKLSAGEALGKQSAEFILETIDYDHIARIK
                     KAIEEKGFKIIEL"
     gene            1636..2016
                     /locus_tag="KPD58_001801"
     CDS             1636..2016
                     /locus_tag="KPD58_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBG0265571.1"
                     /translation="MKHEVIHTNDAPAALGPYSQAIKAGNLLFVSGQVPLVPETMEVV
                     EGDVQAQTAQSLKNLKAILAESGADFSNVVKTTVFIKDMNEFGAINEVYAEYFGENKP
                     ARACVEVARLPKDVKVEIEVIAVL"
     gene            2300..2782
                     /locus_tag="KPD58_001802"
     CDS             2300..2782
                     /locus_tag="KPD58_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG0265572.1"
                     /translation="MLNFFKKNKSYKLHAVVSGNSINIEKVNDSVFSKKLMGDGVAII
                     PNSNVVVAPCNGKVTVLTESKHAFGMVSDEGVEILVHIGIDTVSLQGEGFKNEVSQGD
                     TVKKGSPIISFEREKINSQGIDCTTIIIVLNHSEFSEINCMVENEVVAGQDTVIEIMK
                     "
     gene            2803..3618
                     /locus_tag="KPD58_001803"
     CDS             2803..3618
                     /locus_tag="KPD58_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG0265573.1"
                     /translation="MRVVKIFNNNALSTVTNDRKEAILLGLGIGFNKRPGDKVNEDKI
                     EKIYYVQDHMQTKFLELLKNVSPEVMDASQQIISLIPGNEGKFNNKGILSLVEHISFA
                     IDRMKNNVFLPNLMLLDIKMMYSKEFELGVKALEIIYRVCHIHLPEDEAGYIALHFVN
                     LQSNDNLAYDTLKFVKGSIDLIKECYGLELDESSLSTLRFRTHLKFLAQRIFKNEICQ
                     DDKMIEMYDYLINNHPKNKEYLEKLNVYIEKEFRYKLDKPEKIYLLLHLTKIL"
     gene            3669..5219
                     /locus_tag="KPD58_001804"
     CDS             3669..5219
                     /locus_tag="KPD58_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG0265574.1"
                     /translation="MKEKLLGGLETFAKAMVQPLMYLSSAGILMVVGVLLTNDMLLGV
                     LPFFKWEPIQIFGSLIYNCIMAIINNLAVIFAIGIPAALAKKEKHKAALIGFMSYIIY
                     LTASNTMLTSLGQLAKESKMLGLIGTGQATILGIQVVDTSVFGGIILGCIAGYVFNRT
                     SNKTFKGALQIYSGANFSFICMFFVSILIGVVSNFIWPLAQQGIASLANVIKDTGTFG
                     LFLYGFLERILVPTGLHHLIYTPFQFSDLGGVLKVGADTVSGAYAIVMTEMNLPVERF
                     SDSIYYMATGFTKMFGYIGIGAAFIYTAYKQNKKKVKATIIPLIITAVLASVTEPLDF
                     MFVFCAPFLFFVHAVIAGLFIALLKIFDVTALCGGNLLVSIIMNTTVGVEKTHWPVMM
                     ILGLAQIIVYFVVFSFLIKKFNYKTPGREDESTATGEEQSKQLNLDAGIENIIDGLGG
                     KENINTVENCITRLRVNVKDESKINEDIINLTPNSGIVRKGKDIQIIFGLHVHEVRRA
                     VEDFLEAY"
     gene            5316..6668
                     /locus_tag="KPD58_001805"
     CDS             5316..6668
                     /locus_tag="KPD58_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBG0265575.1"
                     /translation="MIITIVGGGSTFTPGIVKSIALREKELELEEIRLFDIDKERQDK
                     VAVVVNWILKEDVKTDVKLTVTNCEKEAYTDATFIFAQMRVGKYEMREQDEKIPLKYG
                     CVGQETCGCGGMAYGLRTIQPMIKVIDDVEKYAKKDYWILNYSNPAAIVSEACRKLRP
                     NARIINICDMPIAIIDMIAGSLNINDVHNIRYDYFGLNHFGWFTSIDYKHRDLMEEIK
                     EYIKENKILLPKSYIENLNKSRSTGNRHAVSSWIHVWESVYTMLESFPKYLPNTYLTY
                     YLLSKETVEHSNINRTRANEVMESREKDLFEGVRHYLETGEISEKAFYAGSHGDWISD
                     LAVSIKNDTRSRFLVITENRGAIPNMPYDAMVEIPAYIGKDGPEVIARGPIPLFQQGL
                     MMQQLNSEKLIVEAYIENSYEKALQAFTLNKTIPSMNVAKEILDEMIEVNKEYWPELR
                     "
     gene            6799..7557
                     /locus_tag="KPD58_001806"
     CDS             6799..7557
                     /locus_tag="KPD58_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0265576.1"
                     /translation="MNIINKIFWEEDRIGMITNNLEADTHSHCMLQLFLGIEDSIEIT
                     VNEKLVKCNCIIVDKNISHSFSARKKVYYSAIIEPTSIYAEQLTSKMNDFGYWICDND
                     GLEKLRQQGTFLIDNSSKEQYLRFMEMLNNYLNIPITLKHYDDRITELLNLLRTCNCD
                     NHTISSFADKVSLSASRLSHLFKEQIGIPLKSYILFHQMECAFRELLSGKNVTEASMT
                     AGFDTPSHFAGTVKRMMGMPVSLSLKDSEFLKVY"
     gene            7604..8323
                     /locus_tag="KPD58_001807"
     CDS             7604..8323
                     /locus_tag="KPD58_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBG0265577.1"
                     /translation="MNKEYLEETKMLNYNEPQLKLLVSSKNWLELDDFHKIKSIYEFV
                     QNDILFGYNASDMLSATQVLNDGMGQCNTKATLLMALLRAVNIPCRLHAFDVTKDFQR
                     GATSKLISLLAPKYILHTWVEVFYQDRWIALEGVITDKKYLEAIQKKFFNHGGTFKKY
                     AIATNDLKNTSIDWDGKDTFIQKEAIVYDYGIFPSPDVFFSTHSQHMSKLKNFIYVHL
                     IRKIMTKNVCKARNNYIDKNE"
     gene            complement(8447..9847)
                     /locus_tag="KPD58_001808"
     CDS             complement(8447..9847)
                     /locus_tag="KPD58_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBG0265578.1"
                     /translation="METQSNNKKGNLIIAIVMTGAFISSLSQTLLSTALPNIMSDFKI
                     TADIGQWLTTIYLLIAGIIVPTTAYLINRFSTRKLFITSMSIFSIGCIIALFSNNFST
                     MLIARVLQAMGSGSLMPLLQVIILYLCPEEKRGAAMSLVGITVGFAPAIGPTLSGWLV
                     DSFGWHSLFLFLSPIAILDVILSFILLRNVGETQKLKLDIPSIVLSSLGFGGLLIGFT
                     NQGNYGWTNIATYLPILIGIMSLILFTLRQLKSKEPFLELRVFKNKPFLISTILIMIV
                     YASMMSATLMIPLYVQSVRGFSALSSGSLMLPGAILMVVLNPIAGRHLDKYGPHALSI
                     LGTGCLFLGTLSFAFLGRDTSLIHVSLMYCIRMIGISMVLMPLTTWGIKTLDRELISH
                     ATAINNTLRQISGAIGSAILITIMTSATKKAHMSSNMLSNIHGIDVAFSIAATLAFTG
                     LIVSICFIKRHQIIRS"
     gene            complement(9851..10759)
                     /locus_tag="KPD58_001809"
     CDS             complement(9851..10759)
                     /locus_tag="KPD58_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0265579.1"
                     /translation="MKGDNKVTEKQRLIITIAQKIFDQKGFQNTSISDVVKECKMSKA
                     TFYKHFETKESFICEIINYYDEKFLEIIHSINENNDIPSSEKLNRKIIAVWKNIFSRT
                     TINTYIRENFSEEQRKATSKLQTKSRANLLNEYKLSLFDNYGDKIEKIIFDLVFLLDA
                     LIHQFIYIIHVQKREINVYFIAKFTIQILDLVVENMDNLNPLIEKSMFLHKQEDEVYF
                     SLHNKSLFFKTIQDIEELIKTDTSLLENPKLLEAIKKLYVEGKNQQYDSLIMDAMIAY
                     LEKEDTLRPKVLLLNSIKNQLKKETL"
     gene            11020..11667
                     /locus_tag="KPD58_001810"
     CDS             11020..11667
                     /locus_tag="KPD58_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG0265580.1"
                     /translation="MKKKICVAIIFALFIVFVEMFIDSSSEGSKIMTAELSKYRKGVN
                     VLSINNIIVANKKYSLPKDYSPQESSEARDAFYKMNKDAQKSGLNLKAFSTYRSYEYQ
                     DRLFKSYVKEHGEKEANRFSAKPGESEHQTGLAFDIGGDDQSCWANKKFNNTKEAKWL
                     YENAYKYGFILRYPEGKENITGYMYESWHYRYVGTEHSKNFAMNNLTLEEYLHIN"
     gene            11992..14631
                     /locus_tag="KPD58_001811"
     CDS             11992..14631
                     /locus_tag="KPD58_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBG0265581.1"
                     /translation="MSYKSTYQEVIKNLDSSLSGLSKERAEELLEKNGANELKEADKV
                     PTYKLFLESFKDPLVIILLIAALVQIFLGETVESIIIFAVIIINSVLGVVQTKKAESS
                     LESLKNLSAPNAKVIRDNKKMTIPAKNLVVGDIVFLEAGDYIPADGRLIEAQSLKVVE
                     GMLTGESEPVLKHIEKISEDVSLGDQKNMVFSGAVVVYGRGSFVVTGTGMNTEMGKIA
                     GLLESAENKLTPLQQKIEDFSKKLGVGIVILALLIFIIQVARNYFMAGNGEMTQIILD
                     SFMFSVAVAVAAIPEALSSIVTIVLAVGTNSMAKKQAIIRKLPAVETLGSTSVICTDK
                     TGTLTQNKMTVVDYYMYGTNNDELDKEKVNYSYHAKLLTMVSTLCNDSHITSDGKEVG
                     DPTEVALINYSSKNDLDYDKLRSKYIRINEIPFDSDRKLMSTVNSIYGDYIMFTKGAP
                     DIVFSRCKYALKNGSKVDITDEIIEEYKHKNEEFSNRALRVLAFAIKDVPEEDFIPSI
                     DDEHDMTLVGIMAMIDPPREEVMDAVKEAKSAGIKTVMITGDHKTTAAAIAKEIGIME
                     EGDLALTGKELDALSEEQLYEKLENISVYARVSPENKIRIVKAWQHKNNITAMTGDGV
                     NDAPALKQADIGIGMGSGTDVAKDASAMVLVDDNFASIVNAVEVGRTVYSNIIKSITY
                     LFAGNLGAIVSIVFAVFAGWSNPFTALQLLFINLVNDSLPAIALGFEKSEKNIMNKPP
                     RHPDESILAGGTLKAVAIRGCIIGVVTIIAQYIGLQVSPELGVAMAFSTLILARIMQT
                     LPARSNNQTVIKLGFFSNKYVIGAVLVCLGLYSLTLIPVLRGIFSIPTTFGLYQLGIC
                     LGLALISSILMELNKLINRSEVK"
     gene            complement(14689..15792)
                     /locus_tag="KPD58_001812"
     CDS             complement(14689..15792)
                     /locus_tag="KPD58_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HBG0265582.1"
                     /translation="MKLKINKAIEMQLKKSYAIESGHSEDVFEIDCGEGINTVSYSNK
                     AVEAFNALKFDMIRGYPHSIALKDNIVDYWKDFIALDTDRICLADGSIHVIYLLNRLF
                     IEKGDKVLGYSPQFSEYETDIKMHGATYDYVLLKKEDNFKFNEKEFIEKINPEYKVIY
                     IDNPNNPTGQIIPLSSIENIVKEAAKYDIAVMVDEAYGEYMPKENSAVKLLNNYDNVI
                     ALKTFSKGFGLAGLRAGYAVLPEQLVSPIKKISTPYEVSEISRSIAANLLDDVQFIEE
                     LKEKTKDIKNQLLIPWKNLNIAETSDTVSIMTVEHKNKDIDLQQEFAKLKIRVISGSD
                     FTGLDKNFIRFRMPEEKELPEVIKAFQIIDNIE"
BASE COUNT         6057 a         1911 c         2768 g         5264 t
ORIGIN      
        1 tattaatctt aataatgtta aataaaatca aaaaaataga ttttgggtaa ataaaataat
       61 ataaaatata aattttaata aaaataataa agtatatatt attgctttaa taagaatgta
      121 aaaaatattt ttaaattatc atttaatgta atttaaatga attgaagata tatgtttatt
      181 gtatgcaatt gttgtatttt taggtttttt gtgattaatt aaaaaattat aaaaatcgtt
      241 tacatgttga tttttaaaaa aaaaagatat agaattatat agaaagtaat aaacaaaaat
      301 tggatttacg ggagtggaat gaaatgatga atgttacctt aacagatgtg aaagaggcta
      361 gagagaccat taagaacata gtaaaaagaa ctgacttact tgaaagtgtt aaacttagcg
      421 aaaaaacagg agcaaatgtg ttctataaat gtgagaatct tcaaaaaaca ggttctttta
      481 aattaagagg agcttgtaac aaaatagcga gtttaacaga tgaagaaaag gcaaatggag
      541 ttatagcttc aagtgcaggg aatcatgctc aaggagttgc attgggagca aaaatgactg
      601 gtataaaatc tacaatagta atgccagcaa ctgcgccttt agcaaaagtt agtgcaacta
      661 aaggttatgg agcagaagta gttttaaatg gtgcggttta tgatgatgca tatgcaaaag
      721 ctgttgaaat acaaaaagaa acaagagcaa ctttcttaca tccattcaat gataagtatg
      781 ttatagcagg acaaggaact atatctcttg aaattttcga acaattagac aataaagttg
      841 atactatact atgcccagtt ggtggaggag gaatcatctc tggtgttgct gttgcagcaa
      901 aagcattaaa tcctaatgta aaaatagtcg gtgttcaaac tgcaaatata ccttcaatga
      961 aagagtcaat aaaaaatgga aaagtaacaa cagcatttaa tgatactaca atagctgatg
     1021 gtatagctgt taaaactcca ggagatttaa cttttgaaat aataaatgaa ttggttgatg
     1081 aaatagtagt ggttgaagag acagaaattg cagaatcaat attatttatg atggaaagtc
     1141 aaaaaatagt ttcagaaggt gctggtgctg tatgtacagc tgctatatta agtggtaagt
     1201 atgtaccagc aaaagatgaa aatgtagttt gtataatatc tggtggtaat atagacataa
     1261 atacgttata tagaataata ggtgttgctt tagctaaaga aggaagaaga tattcattta
     1321 gtacaattat ggaagataag ccaggaaact ttgctgagtt aactagaata ataagtgaga
     1381 atggtggaaa cattttaagt gctaatcaag gtaaattatc agcaggggaa gctctaggaa
     1441 aacaaagtgc ggaatttata ttagagacaa tagattatga ccatatagct agaataaaga
     1501 aagctataga agaaaaaggt tttaaaatta tagaattata ataaaaagtg tatataatat
     1561 acaagaaaaa tagtataata gtgtatgaag atacatttta tgtgtattag attaacgaat
     1621 taggaggatt ttaaaatgaa acatgaagta atacatacta atgatgcacc agcagcttta
     1681 ggaccatatt ctcaagctat aaaagctgga aacttgttat ttgtatctgg acaagttcct
     1741 cttgtaccag agactatgga agtagttgaa ggagatgtac aagctcaaac tgctcaatct
     1801 ttaaaaaatc ttaaagctat attagctgaa agtggagctg atttttcaaa tgtagttaag
     1861 actacagtat ttataaaaga tatgaatgaa tttggggcta taaacgaagt ttatgctgaa
     1921 tattttggag aaaataaacc agcaagagct tgtgtagaag ttgctagatt accaaaagac
     1981 gttaaagttg aaatagaagt aatagctgta ttataaaaat acaatatttt taatttgaaa
     2041 aggctgaaaa actatattct agtgattcag cctttttata aaaaaataaa tcacaataaa
     2101 gaacatgata aatattgtcg aattaaaaaa taaaattaaa ataaatattg caatattttt
     2161 gagaaggttt tataatgagt atataatttg aaacgtgtaa ctattaaatt aggcatgagt
     2221 tcttggatat ttttatcctg aggattcatg ccttttttgt attgaaattc aaaaatctat
     2281 ttaataagga ggaagtactg tgttaaattt tttcaagaaa aataaaagct ataagttaca
     2341 tgcagtagtg agtggtaaca gcattaatat tgagaaagta aacgattctg tgttttcaaa
     2401 aaagttaatg ggagatggag ttgcaattat tcctaatagt aatgttgttg tagccccttg
     2461 taatggtaaa gtaactgtgc ttacagaatc aaagcatgcc tttggtatgg tctcagatga
     2521 gggtgttgaa atattagtcc acataggaat tgatacagtt agtttacagg gtgaggggtt
     2581 taaaaatgaa gtgtctcaag gagatactgt taaaaaaggt agtccaataa taagttttga
     2641 aagagaaaaa ataaattctc agggaattga ttgtacaaca ataataattg tattaaatca
     2701 tagcgaattt tcagaaataa attgcatggt agaaaatgaa gttgttgcag ggcaagatac
     2761 tgttattgaa attatgaaat aataggaagg gattaattca atatgagagt agtaaaaatc
     2821 tttaataata atgcattatc tacagtaact aatgatagga aagaagctat tttactaggt
     2881 cttggtatag gttttaataa acgtccagga gataaagtta atgaagataa aatagaaaag
     2941 atttattatg ttcaagacca catgcaaaca aaatttttag aattattaaa aaatgttagt
     3001 ccagaagtga tggatgcttc acaacaaatt ataagtctta taccaggaaa tgagggaaaa
     3061 tttaataata aagggatatt atctttagtg gaacatatta gttttgcaat agatagaatg
     3121 aaaaataatg tttttttacc taatttaatg cttttggata taaagatgat gtattcaaaa
     3181 gagtttgaat taggagtaaa agctttagaa attatatata gagtatgtca tatccatttg
     3241 cctgaagatg aagcaggtta catagcttta cattttgtca atttacaatc aaatgataat
     3301 ttggcatatg atactttaaa atttgtaaaa ggtagtattg atttgattaa agaatgttat
     3361 ggattagaat tagacgaatc gagtttaagt acattacgtt ttagaactca tcttaaattt
     3421 cttgctcaaa gaatttttaa gaatgaaatt tgtcaggatg ataaaatgat tgaaatgtat
     3481 gattatttaa ttaacaatca tcctaaaaat aaagaatatt tagaaaagtt aaatgtatat
     3541 attgaaaagg agtttaggta taaattagat aaacctgaaa aaatttatct actactacac
     3601 ctaacaaaaa tcttataaga atgagatgaa ttagtaatag aaatatataa ttttaggggg
     3661 ggataagatt gaaagaaaaa ctacttggag gactggaaac atttgcaaag gcaatggttc
     3721 agccattaat gtatttatcg agtgcaggaa tattaatggt ggtaggtgta ctacttacaa
     3781 atgatatgtt attaggagta cttccatttt ttaaatggga gccaattcaa atatttggaa
     3841 gcctaattta taactgtata atggctatta ttaataatct tgctgttata tttgctatag
     3901 gtattcctgc tgcactagca aaaaaagaga agcacaaagc agcgttaata ggatttatga
     3961 gttatataat ataccttaca gcttccaaca ctatgttaac atcactaggt caactagcaa
     4021 aagaaagcaa gatgcttgga ctgataggga ctggacaagc tactatttta ggtattcaag
     4081 ttgtagatac aagtgtattt ggtggaatta ttttagggtg tatagctgga tatgtattta
     4141 atcgtacaag caacaaaaca tttaaaggag ctttacagat atactcggga gcaaatttta
     4201 gttttatatg catgttcttt gtatccattt taattggagt tgtgagtaac tttatatggc
     4261 cattagctca acaaggaata gcttctcttg caaatgtcat caaagataca ggtacatttg
     4321 gtttattctt atatggtttt ttagagagaa tattagtacc aactggactt catcatttaa
     4381 tctatacacc atttcagttt tctgatttag gaggagtgct taaagttggt gcagatacag
     4441 tttcaggagc ctatgctata gttatgacag aaatgaattt accagttgag cgtttctcag
     4501 atagtatata ctacatggca actggattta caaagatgtt tggttatata ggtataggag
     4561 ctgcctttat ttatactgca tataagcaaa ataagaagaa agttaaggca acgattattc
     4621 cactaataat tacggcagta ttagcctctg taacagaacc attagacttt atgtttgtat
     4681 tctgtgcacc atttttattc tttgtacatg cagttatagc tggtcttttt atagctttat
     4741 taaaaatatt tgatgttact gcattatgtg gagggaactt attagtatca attataatga
     4801 atactacagt aggcgtagaa aaaacacact ggccagtaat gatgatatta ggacttgcac
     4861 aaattattgt atattttgta gtatttagtt ttttaattaa gaaatttaat tataaaactc
     4921 caggtcgtga agatgagagt acagctacag gcgaagaaca atcaaaacaa cttaatttag
     4981 atgcagggat agaaaatata attgatgggt taggcggaaa agaaaatatt aatacagtag
     5041 aaaattgcat cactagatta cgtgttaatg taaaagatga aagtaagatt aatgaagata
     5101 taattaattt aacgccaaat agcgggattg taaggaaagg aaaagatata caaataattt
     5161 ttggacttca tgtgcatgag gtaagacgtg cagtagaaga ttttttagaa gcttattaaa
     5221 gatatatatt tatatagcaa gggtaaataa cagctttaag tgctgtttac tatttattaa
     5281 agatacatat aatttataga ggaggaataa ttattatgat tattactatt gttggtggag
     5341 gaagcacatt tactccaggt attgttaagt caattgcatt aagagaaaaa gagttagagc
     5401 ttgaggaaat tcgcttattt gacattgata aagaacgcca agataaagta gctgtagttg
     5461 tcaactggat actaaaagaa gatgttaaaa cagatgttaa actaactgta actaattgtg
     5521 aaaaagaagc gtatacagat gctacattta tatttgcaca aatgcgtgtt ggtaaatatg
     5581 aaatgcgtga gcaggatgaa aaaattccat tgaaatatgg ttgtgtaggt caagaaactt
     5641 gtggttgtgg agggatggca tacggactgc gtacaataca accaatgata aaggttattg
     5701 atgatgtaga gaaatatgct aaaaaagatt attggatatt aaattactct aatcctgctg
     5761 caatcgtttc tgaagcttgt cgtaagttaa gaccaaatgc aaggattata aatatctgtg
     5821 atatgccaat agctattata gatatgatag cggggtcatt aaatatcaat gacgttcata
     5881 atatacgcta tgattatttt ggtctaaatc attttggctg gtttacttca atagattata
     5941 aacatagaga cttaatggaa gagattaagg aatacataaa agaaaataaa atattattac
     6001 caaaatctta tatagaaaac ttaaataaaa gtcgtagtac aggaaatcgc catgctgtaa
     6061 gtagctggat acatgtttgg gaatctgttt atacaatgtt agaaagcttc ccaaaatatc
     6121 tgccaaacac ttatttaaca tactatcttt taagtaaaga aacagtggaa cattcaaaca
     6181 taaatcgtac tcgtgcaaat gaggttatgg aaagccgtga aaaagactta tttgaaggtg
     6241 tgagacatta cttagaaact ggagaaattt ctgaaaaagc attttatgca ggttcacatg
     6301 gagattggat tagtgatttg gcagtttcaa ttaaaaatga tacacgtagt cgttttttag
     6361 ttataactga aaatcgtgga gctataccaa acatgccata tgatgcaatg gtagaaattc
     6421 cagcatatat tggtaaagat ggtccagaag ttattgctcg aggtccaata cctctatttc
     6481 aacaaggatt aatgatgcaa caacttaaca gtgaaaaatt gatagtagaa gcttatatag
     6541 aaaatagtta tgaaaaagct ttacaagcat tcacattaaa taaaactatt ccaagtatga
     6601 atgttgctaa agaaattttg gatgaaatga tagaagttaa taaggaatac tggccagaat
     6661 taagatagta ggataaagga aagggttttt atttaataat tagatatgtt ttcagagtct
     6721 tttatcaaaa tgtattcaca aacatatggc aatatattaa aataaagagt gatattttta
     6781 gaagaatggg gtgatgcaat gaacattata aataaaattt tttgggaaga agataggatt
     6841 ggcatgatta caaataattt agaggcagat acacatagcc actgtatgtt acaactgttc
     6901 ttagggatag aagacagcat tgaaataaca gtcaatgaaa aattagttaa atgtaattgt
     6961 atcatagtag ataaaaatat ctctcattca ttttcagcaa gaaaaaaagt ttattactct
     7021 gctattattg aacctacatc tatatatgca gaacagctta cctcaaaaat gaatgatttt
     7081 ggctactgga tatgtgacaa tgatggatta gagaaattac gtcaacaagg aacttttttg
     7141 atagataact ctagtaaaga acaatattta agatttatgg aaatgcttaa taactatttg
     7201 aatataccta taactctcaa acattatgat gacagaatta ctgaattgct caatttatta
     7261 cgtacttgta attgtgataa tcatactatt tcaagttttg cagataaagt gtccctatca
     7321 gcaagtagat tatcacatct gtttaaagaa caaataggga tacctttaaa aagctatatc
     7381 ttatttcatc aaatggagtg tgcttttaga gaattacttt ctgggaaaaa tgttaccgaa
     7441 gcttctatga ctgctggatt cgatactcca tcacactttg cagggacagt taagcgaatg
     7501 atgggaatgc ctgtttcttt atctttgaaa gatagcgaat ttttgaaagt atattaaata
     7561 ttctcgttat ataatgaagt tgtactaaga aaggggcttt gttatgaata aagaatattt
     7621 agaggaaaca aaaatgttaa attataatga accacaactt aagcttttgg tatcatcaaa
     7681 gaattggttg gaattagatg attttcataa aataaaatct atttatgagt ttgtacagaa
     7741 tgatatttta tttggatata atgcttctga tatgttaagc gcaacacagg tattaaatga
     7801 tggtatggga caatgcaata caaaagctac tcttttaatg gcattattac gtgcagtgaa
     7861 tatcccttgt aggcttcatg cttttgatgt aacaaaagat tttcaacgag gagctacatc
     7921 caaattgatt tcactattag caccaaaata tattcttcat acttgggtag aggtatttta
     7981 tcaagatagg tggattgctc ttgagggtgt cataactgat aaaaaatatt tagaagcaat
     8041 tcagaagaaa ttttttaatc atggtggaac atttaaaaag tatgcaatag ctacaaatga
     8101 tttgaaaaat acatctattg attgggatgg taaagatact tttatccaaa aagaagctat
     8161 cgtgtatgat tatggtattt ttccatcacc agacgtattt ttttctactc attcacaaca
     8221 tatgtcaaaa ttaaaaaatt tcatatatgt acatcttatc agaaaaataa tgacaaaaaa
     8281 tgtatgtaag gctagaaaca attatattga taaaaacgaa tgagtgttta agaggtaact
     8341 atataggaaa tatatagaat aattaaaact gtactggtta aaatgaataa aaaggagtta
     8401 tcacagagca aacttaaatt gttcatagga taactccttt tctgtattaa cttctaataa
     8461 tttgatgtct ttttataaaa catatagata caattaaacc agtaaatgca agagtagctg
     8521 ctattgaaaa tgcaacatct attccatgaa tgttagacag catgtttgaa ctcatatgag
     8581 ctttcttagt agcacttgtc attatagtaa taagtatagc agaacctata gctccagata
     8641 tctgacggag tgtgttatta attgctgtgg catgagatat aagttctcta tctaatgttt
     8701 taattcccca tgtagttaat ggcattaaaa ccattgaaat accaatcata cgaatacaat
     8761 acatcaatga cacatgaatt aaagatgtat ctctgcctaa aaaagcgaag gataaagttc
     8821 ctaaaaacaa acatcctgtt cctaatattg atagagcatg aggtccatat ttatctaaat
     8881 gacgtcctgc aattggattg agtacaacca tcaatatagc accaggtaac attaatgaac
     8941 ctgaagataa tgcagaaaat cctcttacag attgaacgta cagaggaatc attaatgtag
     9001 cagacatcat tgaggcatat acaatcataa ttaatattgt agaaataaga aatggtttat
     9061 ttttaaaaac cctcaattct aaaaaaggtt ctttagattt taattgacgc aatgtaaata
     9121 aaattaatga cataattcct attaatattg gtaaatatgt ggcaatatta gtccacccat
     9181 aattaccttg attagtaaat ccaattaata aaccaccaaa acctagagat gatagtacta
     9241 ttgaaggtat atctaatttt agtttttgag tttccccaac atttcttaat agaataaatg
     9301 atagtataac atctaatata gcaatagggg aaagaaacag aaataaacta tgccaaccaa
     9361 atgaatctac taaccatcct gataaagttg gacctattgc tggagcaaat cctactgtaa
     9421 taccaactaa actcatagct gcaccacgtt tttcctcagg gcataaatag agtataataa
     9481 cttgtaaaag tggcattaga gaaccagaac ccattgcctg caacacacga gcaatcaaca
     9541 tagttgaaaa attattagaa aaaagtgcaa taatacatcc tatagaaaag atagacattg
     9601 atgtaataaa taactttcta gtactaaatc gatttattaa ataagctgtt gtgggtacaa
     9661 taatacctgc aataagtaga taaattgttg ttaaccactg accaatatca gcagtaattt
     9721 taaaatctga cattatatta ggtaatgctg ttgacaatag tgtttgactg agagaactta
     9781 taaaagcacc agtcattaca attgctatta ttaaatttcc ttttttattg ttactttggg
     9841 tttccataat ttataacgtc tcctttttta attgattttt aatgctgttc aataaaagta
     9901 cttttggtct taaagtgtct tctttttcta aatatgcaat cattgcatcc ataattaaag
     9961 aatcatattg ttgatttttt ccttcgacgt ataatttttt tattgcttct agtaatttag
    10021 gattttcaag gagagaagta tcagttttga ttagttcttc tatatcttgt atcgttttaa
    10081 aaaatagcga tttattatga agggaaaagt aaacttcatc ttcttgttta tgtaaaaaca
    10141 ttgatttttc aattaaagga tttagattat ccatattttc tacaactaaa tctagaattt
    10201 gtatagtaaa tttggcaata aagtatacat ttatttctct tttttgtaca tgaataatgt
    10261 atataaactg atgaattaga gcatccaata agaaaactaa atcaaatata attttttcta
    10321 ttttatcacc atagttatca aataagctta gtttgtattc attcaataag ttagctcttg
    10381 attttgtttg caatttactt gtagccttac gttgctcttc agaaaagttt tctcttatat
    10441 aggtatttat ggtagttcta gaaaaaatat ttttccatac agcaattatc tttctgttta
    10501 acttttctga tgatggaatg tcattatttt catttatgga atgtataatt tctaaaaact
    10561 tttcatcata ataattgatt atttcgcaga taaaactttc cttagtttca aaatgcttgt
    10621 aaaaagtagc tttagacatt ttacactctt ttacaacatc cgatattgaa gtattttgaa
    10681 aacctttttg gtcaaaaatt ttttgtgcaa tagttataat gagcctttgt ttttctgtca
    10741 ctttattatc tcctttcacg caaaaacgtt tagtactata tagtacctac aagttttgat
    10801 aaatatatta tacatcattt ggaaaaaaaa tcaatatata gactgtaata tgaaattttt
    10861 gaaaaatttg ttgttatttt tagtcgtata aaaaagaaac ttaagagttt cttaagaatt
    10921 gtttaaagaa gttgcaataa attagtggta tgattatagt atgaaagaga atgtcttgaa
    10981 atttttctgt atcaaattcc aattatggag gtgtagtata tgaaaaagaa aatttgtgta
    11041 gcaataattt ttgctctatt tatagttttt gtagaaatgt ttattgatag ctctagcgaa
    11101 ggaagtaaaa ttatgacagc tgaattaagt aaatacagga aaggcgtaaa tgtattatca
    11161 ataaacaata ttatagtagc gaataaaaaa tatagtttgc ccaaagatta ttctcctcag
    11221 gaaagcagtg aagcgagaga tgctttttat aaaatgaata aagatgctca aaagtcagga
    11281 ttaaatttaa aagcttttag tacatataga agttatgaat atcaagatag attatttaag
    11341 tcatatgtaa aagaacatgg agaaaaagaa gcaaatagat tttcagctaa acctggagag
    11401 agtgagcatc aaactggact tgcttttgat attggaggag atgaccagtc ttgttgggca
    11461 aataaaaagt tcaacaatac aaaagaagct aagtggttgt atgaaaatgc atataaatat
    11521 ggttttatac ttagatatcc agaaggtaaa gagaatataa caggatatat gtatgaatct
    11581 tggcactata gatatgttgg aactgaacat agcaaaaact ttgcaatgaa taatttgact
    11641 ttagaagaat atttacatat aaattaagag atggtttgtt aaaattaggt taaaataaaa
    11701 aataacctag actattagct gtgtaaagtg atataatgta aatacaattt aagataacta
    11761 ctcgaagggg agtagcttac acgaaagtgg taattaatcg tcaagacagt agaaatactc
    11821 ggttaattag caagggattc cttgatagca agaccttcaa aataagcatt aggtatgttt
    11881 gttttggggt ctttttttat tcaaggaatt tttatttaaa aaaatttaaa tttcaagtat
    11941 acataagagc ccccaaacat aaaataaatt tattgaagag gagggcaaca aatgtcttac
    12001 aaaagtactt atcaagaagt aattaaaaat ttagactcaa gtttatctgg tttgagtaaa
    12061 gaaagagcag aagagttgtt agaaaaaaat ggagctaatg aattaaaaga agcagataag
    12121 gttcctactt ataagctttt cctagaaagt tttaaagacc cattagtaat aattctattg
    12181 atagctgctt tagtacaaat atttttaggt gaaacagtgg agtctataat aatttttgca
    12241 gttattatta taaactctgt tctgggtgtt gtacaaacaa aaaaagcaga aagttcatta
    12301 gaaagtttga aaaatttatc tgcaccaaac gcaaaggtaa taagagacaa taaaaaaatg
    12361 acaattccag caaaaaatct agtagttgga gatattgtat tcttagaagc tggcgattac
    12421 ataccagcag atggaaggct tatagaagca caatctctca aggttgtaga agggatgtta
    12481 actggagaat cagaaccagt tttaaagcat atagaaaaaa taagtgaaga tgtaagttta
    12541 ggtgaccaga aaaatatggt atttagtggt gctgtagttg tttatggtag aggttctttt
    12601 gtagtgactg gaacaggtat gaatactgag atgggtaaaa tagctgggct tttagaaagt
    12661 gctgaaaata aacttacacc attgcaacaa aaaatagagg attttagtaa aaaacttggt
    12721 gtaggtattg taatacttgc tcttttaata tttataattc aagtagcaag aaattacttt
    12781 atggctggaa atggagaaat gacacaaatt attttagatt cattcatgtt ctctgtagca
    12841 gtagcagtag cagctatacc agaagctcta tcatcaatag tcactatagt attggctgtt
    12901 ggaacaaata gtatggcaaa aaaacaagcc ataataagaa aattaccagc agtagaaact
    12961 ttaggttcaa caagtgtaat atgtactgat aaaacaggta ctcttacaca aaataaaatg
    13021 acagtagttg attattatat gtatggaact aataatgatg agttagataa agaaaaagta
    13081 aactattctt atcatgctaa attattgact atggtttcta cattgtgtaa tgactctcat
    13141 ataactagtg atggtaaaga agttggtgac ccaacagaag tggctttaat aaattattca
    13201 agtaaaaatg atttagatta tgataaatta agaagtaagt atatcagaat aaatgaaata
    13261 ccatttgact cagacagaaa gcttatgtct actgtaaata gtatatatgg agattatata
    13321 atgtttacaa aaggtgctcc agatattgtg tttagtagat gtaaatatgc tttgaaaaat
    13381 ggttcaaaag tagatataac agatgagata atagaagaat ataaacataa aaatgaagag
    13441 ttctcaaata gagcacttag agtattagct tttgcaataa aagatgtccc agaagaagat
    13501 tttatacctt caattgatga cgaacatgac atgacattgg ttggaataat ggcaatgata
    13561 gacccaccaa gagaagaagt aatggatgca gtaaaagagg caaagagtgc tggtataaaa
    13621 actgttatga taactggtga ccataaaaca acagcagcag caattgcaaa agagattggt
    13681 ataatggaag aaggagactt ggctcttact ggaaaagagc ttgatgcttt gagtgaagaa
    13741 caactatatg aaaaactaga aaatataagt gtatatgcca gagtctcgcc agaaaataaa
    13801 attcgtatag taaaagcatg gcaacataag aataatataa cagcaatgac tggtgatggt
    13861 gttaatgatg cacctgcatt aaaacaagca gatataggaa tcggtatggg aagtggtact
    13921 gatgtagcaa aagatgcttc tgctatggta ttggttgatg ataactttgc aagtatagta
    13981 aatgctgttg aagttggtag aacagtatac agtaatataa taaaatcaat tacttatcta
    14041 tttgcaggaa acttaggagc tatagtatcc atagtatttg cagtgtttgc aggttggtca
    14101 aatccattta cagctttaca acttttgttt ataaacttag taaatgactc tttaccagca
    14161 attgcattag ggtttgaaaa gtcagaaaaa aatataatga ataaacctcc tagacacccg
    14221 gatgaaagta tactagcagg aggaacattg aaggcagttg ctataagagg ttgtataata
    14281 ggtgtagtta caataattgc acaatatata ggattacaag tatctccaga acttggagta
    14341 gcaatggcat tttcaacatt gatattagct agaataatgc aaacattacc tgcacgttct
    14401 aataatcaaa cagtaataaa attaggtttt ttcagcaata aatacgttat aggagcagtt
    14461 ttagtctgtc ttgggcttta ctctttaaca ctaataccag tgctaagagg aatattctct
    14521 ataccaacaa cgtttggatt gtaccagttg gggatttgcc tagggttagc attaatttca
    14581 tcaatattaa tggaacttaa caaattaata aatagaagtg aagtaaaata atcaaaaaat
    14641 taaagggcat attgatttta acaatatgcc ttttataaat agtctaattt attctatatt
    14701 atcgattatt tggaatgctt ttataacttc aggtaattct ttttcttcag gcattctaaa
    14761 tcttatgaaa tttttatcaa gtccagtaaa atcacttcca gatataactc ttatttttaa
    14821 tttagcaaac tcttgttgta aatctatgtc cttgttttta tgttcaactg tcataattga
    14881 tacagtatca ctagtttcag ctatattaag gtttttccat ggaattaata attgattttt
    14941 tatatctttt gttttttctt ttaattcttc tataaattga acatcatcta ataaatttgc
    15001 tgcaatacta cgagatattt cagacacttc gtaaggagta gaaatctttt taatagggct
    15061 aacaagttgt tctggaagaa ctgcatatcc tgctcttagt cctgctaaac caaatccttt
    15121 tgaaaatgtt tttaaagcaa ttacattatc ataattattt agaagcttga cagctgaatt
    15181 ttcttttggc atgtattcac cataagcttc atctaccata actgctatat catatttagc
    15241 tgcttccttt actatatttt caattgatga taaaggtata atttgtccag taggattgtt
    15301 tggattgtct atgtaaatta ctttatattc tggatttatt ttttcaataa attctttttc
    15361 attaaacttg aaattatcct ctttctttaa aagaacataa tcataagttg caccatgcat
    15421 ttttatatct gtctcatatt ctgagaactg aggtgaatat cctaaaactt tatctccctt
    15481 ttcaataaat agtctattta aaaggtaaat aacatgtata gaaccatctg ccaaacaaat
    15541 tctgtcagta tctaatgcta taaaatcttt ccaataatca actatattat cttttaaagc
    15601 aattgaatga ggatatcctc ttatcatatc aaattttaaa gcattaaaag cttctacagc
    15661 tttgtttgag taagacacgg tatttatacc ttcaccacag tctatctcga atacatcttc
    15721 tgaatgtcca gattctattg cgtaactttt tttcaactgc atttcaatag ctttgttaat
    15781 ttttagtttc atactttatc actccttatt tagtcaataa taaatgttat aaaagttgct
    15841 agatttaaaa tattatgaaa atatataata gtaaaaaatg aatataaata ttataatagc
    15901 tcactttatt taatatgatt atatatcata tattgtaaaa tttctagcat tataagacta
    15961 taaattgaaa atattcagaa aaatactaca agaaacgatt
//
LOCUS       DAELLV010000072        15643 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.072, whole genome shotgun sequence.
ACCESSION   DAELLV010000072 DAELLV010000000
VERSION     DAELLV010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15643
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.072"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..775)
                     /locus_tag="KPD58_001813"
     CDS             complement(<1..775)
                     /locus_tag="KPD58_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0265583.1"
                     /translation="MRNKIFLSILIIISLLIGCDKKSLLSIHMIDVGQGDSILVQTPT
                     NKNILIDGGDEDSENIIISYLRQKRIKTIDIIIATHPDSDHIGSLDNIIKKFNVNSIY
                     MPEQSTDSKAYQNLINSCTDKNLSIQHLYKNDVLNIDNNINIYVLSPSYIQEESNLNS
                     IVFKLTFNDNSFLFMGDAEEENEKEILHSFKLNNINFIKIGHHGSNSSSSLEFIKKIS
                     PDIAAISCGYKNQYGHPHREVINNLKQNHVSIYRTDRIGD"
     gene            963..1868
                     /locus_tag="KPD58_001814"
     CDS             963..1868
                     /locus_tag="KPD58_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HBG0265584.1"
                     /translation="MFFKDIELNSKKELDPYFDLVDYEACEYCFSTLYMWQHVYKTGY
                     YIGEDFAVLVGEYEGDSFSILPLAKKDKLPEVVDFVLEYFSKNNKKIYLRGITTEVVE
                     FLKEKYPGRFEYIEERDLFDYIYDAESLRTLAGKKNQKKRNHINYFLKEYAGRYEAKL
                     LDKENFDECLVLMKEWESNKEENNEFDESMDDELIGIKKIFNHYDILKDKVKVFGVYV
                     DGKLEAFSIGELLNPNMALIHIEKANPDIRGLYPFINQQFLVSEFKDVEFVNREEDLG
                     IEGLRKAKLSYHPCRFVEKYSVREA"
     gene            1877..3061
                     /locus_tag="KPD58_001815"
     CDS             1877..3061
                     /locus_tag="KPD58_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0265585.1"
                     /translation="MEIRYAKEEEIESIKDIWSYCFNDTESFMKYYFNDKYKSENTVV
                     ALDEGKIISSLQLNQYKLLLNSKVYNTSYVVGVSTLPEGRGAGYMNKVMKFTLNELYK
                     KGQLVSILMPIDYRLYRRFGYEHCYDQIEYTINTDDLKNFKSSGKMIKSNLSQIDDLI
                     QIDRTFLNEVNGNVLKDEHYYENLFKEIQSEDGFLYIHEGNEKDGYIVYFLQEDKMFV
                     RELFYKNIDALKSMLKFIYNHNTQCKIVTISTPTIDKIRFILDNPKDSDIKIKPFMMG
                     RVINVKKFIEDIDIEKDINSSFNLLIEDKFIDENNGLFKISIQNKKVSVEQLDKKGAE
                     KPQEDFDIKLDINTLTQLSFSYIDVNEAIFLNDIKDVSEETLETLNCIFSKKNNYINE
                     YI"
     gene            3176..4477
                     /locus_tag="KPD58_001816"
     CDS             3176..4477
                     /locus_tag="KPD58_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HBG0265586.1"
                     /translation="MGVIIIQIRRPLLIILMFVVAISFIITNNTKNDEELNDKIITIQ
                     GVVKGKIEKKRYNQYKVGVFLINDYTKNKNLKIGQKVNITGKFKSLDKMKYDDFDYGR
                     YIKSTGYKGIVYINNYKIIDKNKLYSLIGEIKFYISKTYRYLYKKNSDFINSILLAEV
                     ENLTEEQKEIFTRTGTSHVISISGLHTGILCVIISFLLRGINKLYKLLILSIFITLYC
                     IMVGASPSIIRSIAFVMIFYLSIFIDRKKDGISALSLIGIILIMNNPYVIYNVSFQLS
                     FLATLSILIFYNKINSIIKLSMVSLTISSNILTLPIIYYTFKGIPLLSIIGNLIIVPF
                     VGVIMYLSIASLIVFKVSVVIAKIISFFNSTFIESIFFLLEKISNLSFAYINIENPKF
                     YIVVIYYIGVFFYIFYIEGKEIKEQENELQGYYKECKREKL"
     gene            4431..5474
                     /gene="holA"
                     /locus_tag="KPD58_001817"
     CDS             4431..5474
                     /gene="holA"
                     /locus_tag="KPD58_001817"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBG0265587.1"
                     /translation="MNYKDIIRNVKEKNFEKMYLFYGREYYLIENAIKAFKDSLNEGM
                     LDFNLDIIDGKEIVLNQLISSIETLPFMDDRKIVIIKDFELLKGKKKNFTDSDEKYLI
                     EHLDNIPDTTTIVFVVYGDVDKRKSLVKKIGNNGIVFDCDKLSDMDLFKWIKKSFALN
                     DVIIDNSQIMYFIEQEGYRDKSSEKTLSDLENEINKISSFVAKGNNVTNDVIDKLSQK
                     KVENDIFKLIDYIGEQNASNAMKILNDMIQEGESVLGIFSMIARQFKIIMQVRQLQLD
                     GYSTKLIADKLKMHQFVVGKALKQTKNFSDDIIVEILNYILESDYKIKTGLIRDTLAV
                     EMLVSRYCKREAI"
     gene            complement(5661..5927)
                     /gene="rpsT"
                     /locus_tag="KPD58_001818"
     CDS             complement(5661..5927)
                     /gene="rpsT"
                     /locus_tag="KPD58_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBG0265588.1"
                     /translation="MANIKSAKKRISVIEKKTALNRVRKSQIKTAIRRFEDAVAAGNR
                     EDAVAKFQYAQKRIYQVASKGTIHKNAAARKVAKLAQKLNGMNA"
     gene            6523..7044
                     /locus_tag="KPD58_001819"
     CDS             6523..7044
                     /locus_tag="KPD58_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0265589.1"
                     /translation="MDIAKLILNPARLRILQYIRLYGSVRTSDIVKYLNDIPRATVYH
                     HVKILEENNMIEVIKENRVRGTIEKVYALKEYTTSMEGETFVALSTAFYVGLMQEMNE
                     YFSRKEQDHKKDNVFFSSALLYMSDNEYENLLKSIADLLKPYIEQKPKSNLKLRKLSI
                     ISSPPVADNDKTK"
     gene            7078..7854
                     /locus_tag="KPD58_001820"
     CDS             7078..7854
                     /locus_tag="KPD58_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0265590.1"
                     /translation="MKFHEFGNKNRPHIMLIHGGGNAWWNYLRQAEVLSNKYHVILPT
                     LDGHGEEYETTYISTEDTADKLISYIDENCSGHLFALCGVSLGGQIVMELLSRKPNIT
                     KKAIIDGSICYPRPLMARFCIAGIRFFGGLLFSEKACRFQIAMMPKLLPENMQYPDEI
                     KAYYMTDMPHVRKETFYNMYRTYMMNYTLKENVKKTTAQVMYWYGEKEMKCVKKSAKL
                     FQSYVPSCEIYEAKSYNHGYLSLYLPNEWLEIAEPFFQKE"
     gene            8196..9149
                     /locus_tag="KPD58_001821"
     CDS             8196..9149
                     /locus_tag="KPD58_001821"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HBG0265591.1"
                     /translation="MISVRTDLALEASEMCEKSQEGSSIPGVKIETKELENCIVTKVE
                     VIDRQGAEIMNKDIGKYITLESNLMKFDDDESREEMISYLKEELVDIFGQDKNKKTLV
                     IGLGNRNITSDALGPKSVSKTLVTRHLFKNYNKDYDDDFTEVSALSPGVMGVTGIETS
                     EIVKSLVEKVKPDRVVAIDALASRKMERVNSTIQISTAGISPGGGVGNTRKSLTKETL
                     GVDVIAIGVPTVVDAATLTIDVLDMAIDNLIAQSEETESFYEMLKKLKEEEKYHLIKD
                     SLDPYDKNLIVTPKDIDDTIENLSIIISEGLNRSLHPGRLV"
     gene            9167..10186
                     /locus_tag="KPD58_001822"
     CDS             9167..10186
                     /locus_tag="KPD58_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HBG0265592.1"
                     /translation="MFKKCIKVVTLTFILVCILPGKSLALNQDDFLKFLVNSSYPEAK
                     VEGNDTENKKNNKNKETSKENKEESKEGNTKSKDASKVDNKKESEKEYIKLYVGKENV
                     PDIESKNSDTTETNTTSSSDYKDDLRVTKENPRILIYHTHGCETYSNSPDGNYHSRDK
                     KNSVMEVGSALTSALDSKGWGVVHTTKYHDYPSYNNSYASSLKTIQSILPKYNSVDIA
                     IDLHRDARDLTNPATKEKDHLKYTTMINGERVSKFFFVVGGKNTNRKQLRALAEDITA
                     FAEKKYPGLVSPIVEKDYARFNQFAVKNHMLVEIGNNATSVEESKATTKYLAEILDEY
                     FKQKN"
     gene            10217..10468
                     /locus_tag="KPD58_001823"
     CDS             10217..10468
                     /locus_tag="KPD58_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265593.1"
                     /translation="MSRLEKNIESKSKLRKYKYIMRILFLITMIICTSVCVFIVDSNA
                     KSMLGMKQYDFPSIKFDITKFDFDIPNFDIHEIIKKFNK"
     gene            10750..12555
                     /gene="lepA"
                     /locus_tag="KPD58_001824"
     CDS             10750..12555
                     /gene="lepA"
                     /locus_tag="KPD58_001824"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBG0265594.1"
                     /translation="MDNKQSRTRNFSIIAHIDHGKSTLADRLIQQTGLVSERDMKSQL
                     LDNMDLERERGITIKLQNIRLMYKAKDGNEYYLNLIDTPGHVDFNYEVSRSLAACEGA
                     LLVVDAAQGVEAQTLANVYLAIDQDLEILPIINKIDLPSARPEEVKNEIEDLIGLDSS
                     EAPLISAKTGLNIEDVLEDIVKNVPPPKGDNEAPLKALIFDSYYDAYKGVVAYVRVFE
                     GTVKKGMTIKMMNTNKKFEVTEVGVMAPGQTELSELSAGDVGYIAASIKDIRSCRVGD
                     TITDSNNPTEEPLPGYKKATPMVYCGIYPGEGEKYENVRDALEKLQVNDAALEYEAET
                     SAALGFGFRCGFLGLLHMEIMQERLEREFNLDIITTAPSVIYRVTKMDGEVVMIQNPA
                     NLPEPSEIKMIEEPIVKGDIIVPKDYVGVVMELCQERRGNMLNMEYIDDRRVMLHYDL
                     PLNEVVYDFFDALKSRTRGYGSLDYEVKGYVASTLVKLDILINKEQVDALSFIVHETR
                     AFPRGKAMCEKLKGEIPRHQFAIPIQAAVGNKVIARETISALRKDVLAKCYGGDISRK
                     KKLLEKQKEGKKRMRQIGSVEVPQKAFMSVLKLDE"
     gene            12704..14041
                     /locus_tag="KPD58_001825"
     CDS             12704..14041
                     /locus_tag="KPD58_001825"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HBG0265595.1"
                     /translation="MINIEIPKKVDYIIKELEKNGYEAYIVGGCVRDCLLERVPNDWD
                     ITTSARPEVVVELFEKTIPTGIQHGTVTVMIEHEPFEVTTYRIDGNYSDGRHPDSIEF
                     TNNIVKDLSRRDFTINSIAYNSKTGLVDPFNGYEDIQNKYIRCVGNPVDRFEEDALRM
                     LRAVRFSAQLNFKIAEGTKQSIHKKADLIKNVSIERIQTEFNKILVSDSSKLNLLKST
                     GLLKFIIPEICELEDVTQHNPYHIYDVQKHTLIATEAIEDELYLKLTMLFHDLGKKVT
                     KTTDKNGVDHFYTHSRESVKIAKKILKRLKYDNYTINKVLILIQYHDYRIEPKRKIIK
                     KLLNKLEDVELFEDLIKVNWADTLAKNPKYAKQKILNLIECEKEFKHIINQKECFNLK
                     DLAINGKDLISIGIKPGKDIGHILNKMLEIVINNPELNEKEILKEKALNIYTF"
     gene            14274..15587
                     /locus_tag="KPD58_001826"
     CDS             14274..15587
                     /locus_tag="KPD58_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG0265596.1"
                     /translation="MHTQLQKTIGLSAALSTVVGMVIGSGVFFKPQAIYTTTNGAPGL
                     GIIAWLLGGFITITAGLTATEISAAIPKTGGMMIYIEEIYGEKLGFLTGWMQTVLFFP
                     GTSAALGVIFAQQASELLGMSPNNMANVLPIAVGVILFLALLNIIGSSLGGKVQTVAT
                     IGKMIPLILIIVFGFIKGQSSEVLNPFVGDGVNASNALGQVLIATLFAYDGWINVGAI
                     SGEMKSPEKDLPRAIVGGLSLVMAVYIIINIAYLWVVPASELATVTSPATLVATRLFG
                     NIGGKVITVGILISVFGTLNGYLLTGSRIPYTLAEMGTLPASKTLLKVNSGGSPVNSI
                     LLITVLACVYALSGQFNLLTDLTIFSIWVFYVLTFIGVMRLRRQKPDLHRPYKVPLYP
                     IIPIIAILGGLFVIINQILTSTVISLGGIFITLLGLPVYYYMKKR"
BASE COUNT         6156 a         1657 c         2604 g         5226 t
ORIGIN      
        1 tatctcctat tctatctgtt ctataaattg atacatggtt ttgttttaga ttattaataa
       61 cctccctatg tggatgacca tattgatttt tatatccaca tgatattgca gctatatctg
      121 gactaatttt ttttataaat tctaaagatg aagaagaatt acttccatga tgacctattt
      181 ttataaaatt aatattattt aatttaaaag aatgtaaaat ttctttttca ttttcttctt
      241 cagcatcacc cataaataaa aaagaattat cattaaaagt taatttgaaa actatagaat
      301 ttaaattaga ttcttcttgt atatatgatg gacttaatac atatatattt atattgttat
      361 ctatgtttaa aacatcattt ttatagagat gctgaataga taagttttta tccgtacaag
      421 aatttattag attttgataa gccttactat cagttgattg ttcaggcata tatatggagt
      481 ttacattaaa tttttttata atattatcaa gacttcctat gtggtctgaa tctggatgag
      541 ttgctattat aatatcaata gtttttattc ttttttgtct aagataacta ataattatat
      601 tttcgctatc ttcatcacct ccatcaatta atatattctt atttgtgggt gtttgaacta
      661 aaatactatc accttgtcca acatcaatca tatgtataga tagtagagat tttttgtcac
      721 agccgattaa aagtgatatt ataataagta ttgataaaaa aattttattt ctcaataaaa
      781 aatcactcca ttcattacat gtttatataa atttatgatg ggtaatataa gatatatatg
      841 acttattata ttttatattt taaacaatat aaatgaaatt aataccataa taaaattgga
      901 gttttttata tatacaaaat aaaattttta aaattaagca ataaaaaagg taggaggatt
      961 ttatgttttt caaagatatt gaactaaatt caaaaaaaga gttagaccca tattttgatt
     1021 tagtagatta tgaagcttgt gagtactgtt ttagtacttt atatatgtgg caacatgttt
     1081 ataagacagg atattatata ggtgaagatt ttgccgtttt agttggggaa tacgaaggag
     1141 atagcttctc aatattacca cttgctaaaa aagataaact tccagaagtt gtagattttg
     1201 tccttgaata tttttctaaa aataataaaa aaatatactt aagaggtatt actactgaag
     1261 tagtagagtt tttaaaagaa aaatatccag gtagatttga gtatatagaa gaaagagatt
     1321 tatttgacta tatttatgat gcagaaagtt taagaactct agctggtaaa aagaatcaaa
     1381 agaagagaaa ccatataaac tatttcttaa aagagtatgc aggtagatat gaagctaaat
     1441 tattagataa agaaaacttt gatgagtgtt tagtactaat gaaggaatgg gaaagtaata
     1501 aggaagaaaa taatgaattt gatgaaagta tggatgatga attaataggt atcaaaaaga
     1561 tatttaacca ttatgacata ttaaaagata aggtaaaagt gtttggtgtt tatgtagatg
     1621 gaaaattaga agcttttagt ataggagagc ttttaaatcc aaatatggca ttgattcata
     1681 tagaaaaagc taatcccgat ataagaggtc tgtatccatt tataaatcaa caatttttgg
     1741 taagtgaatt taaggacgtt gagtttgtaa atagagaaga agatttaggt attgaaggat
     1801 taagaaaagc taaactttct tatcatcctt gcagatttgt tgaaaagtac agtgttaggg
     1861 aggcttaata gactatatgg agattaggta tgctaaagaa gaagagatag aaagtataaa
     1921 agatatttgg agttattgtt ttaacgatac ggagagtttt atgaagtact attttaatga
     1981 taaatataaa tctgaaaata ctgtagtagc tttagatgaa ggaaaaatta tttcttctct
     2041 tcagctaaat cagtataagt tattactaaa tagcaaggta tataatacat catatgtggt
     2101 aggagtttct acacttccag agggaagagg agctggatat atgaacaaag taatgaaatt
     2161 tactttaaac gaattgtata aaaaagggca attagtttct atattgatgc ctattgacta
     2221 tagactttat agaagatttg gatatgagca ttgttacgac cagattgaat atactattaa
     2281 cacagatgat ttaaaaaatt ttaagtcaag tggaaaaatg ataaagtcta atttatcaca
     2341 aattgatgat ttgatacaaa tagatagaac ttttttaaat gaggttaatg gtaatgtgtt
     2401 aaaagatgaa cattattatg aaaacttatt taaagagatt caaagtgaag atggattttt
     2461 atacattcat gaaggaaatg aaaaagatgg atatatagtt tattttcttc aggaagataa
     2521 aatgtttgtc agagagttat tttataaaaa tatagatgcc cttaaatcaa tgcttaaatt
     2581 tatatataat cacaatactc aatgtaagat agttactata tctactccta caatagataa
     2641 gataaggttt atattagaca accctaaaga tagtgatata aaaataaaac cttttatgat
     2701 gggaagagtt ataaatgtaa agaagtttat tgaagatata gatattgaga aggatattaa
     2761 tagctcattt aatttattaa tagaagacaa gtttatagat gaaaataatg gtctatttaa
     2821 aatttctata cagaataaga aggtatctgt agagcagtta gataaaaaag gtgcagaaaa
     2881 gcctcaagaa gattttgata ttaaattaga tataaatacc ttaacacagt tatcattttc
     2941 ttatatagat gtaaatgaag ctattttctt aaatgatata aaagatgttt cagaagaaac
     3001 actagaaaca cttaattgta ttttttctaa aaaaaataac tatataaatg aatatatata
     3061 ggtagagtac gttaaaagat tttaaaaatc ttttgacgta tttttttgtt tagataacaa
     3121 tttatcttgt ttaaaaattt tctatttgaa aataatctaa ataatatact tttatatggg
     3181 agtgattata attcaaataa gaagaccatt attaattata ttaatgtttg tggtagctat
     3241 atcatttatc attacaaata acacaaaaaa cgacgaagaa cttaatgata agataataac
     3301 tatacaagga gttgtcaaag gaaaaattga aaagaaaaga tacaatcagt acaaggtagg
     3361 ggtttttcta ataaatgatt atacaaaaaa taaaaatttg aagattggtc agaaagttaa
     3421 tattacaggt aaattcaaaa gtttagataa aatgaagtat gatgattttg attatggaag
     3481 gtatataaaa agtactggat ataaaggtat agtgtatata aataactata aaattattga
     3541 taaaaataaa ctttactctt taatagggga aataaagttt tatattagta aaacttatag
     3601 gtacttatat aagaaaaact cagattttat aaattctata ctattagcag aagttgaaaa
     3661 tttgactgag gaacaaaaag aaatttttac cagaactgga actagtcatg taatatctat
     3721 atcaggtctt catacaggaa tattatgtgt aattatatcc tttctattaa gaggtataaa
     3781 taaattgtat aaattattaa tactgtcgat ttttataact ttatattgta taatggtagg
     3841 tgcgtctcca tccataatta ggtctatagc atttgttatg attttttatt tatcaatatt
     3901 tattgataga aagaaagatg gaatatcggc attgtctcta attgggataa ttttaattat
     3961 gaataacccc tatgttatat ataatgtaag ttttcaattg tcttttttag ctacactttc
     4021 tatattaata ttctataata aaataaatag tattataaaa ttaagtatgg tatcattgac
     4081 aatatcatca aatatattaa ctttacctat aatttattat acatttaaag gtataccatt
     4141 gctttcaatt ataggaaatt taattatagt gccatttgtg ggagtaataa tgtatttaag
     4201 tattgcaagt ttaatcgtat ttaaagtaag tgtagtaata gctaaaatta tatctttttt
     4261 taatagtaca tttatagaaa gcatattttt tttactagaa aaaatcagca atttaagttt
     4321 tgcatacatt aatatagaaa atcctaaatt ttatattgtt gttatttatt acataggagt
     4381 gtttttttac atattttaca tagaaggaaa agagataaag gagcaagaga atgaattaca
     4441 aggatattat aaggaatgta aaagagaaaa actttgaaaa aatgtatctt ttttatggaa
     4501 gagagtatta tttaattgag aatgcaataa aagcatttaa agatagtcta aacgagggaa
     4561 tgcttgattt taatttagat ataatagatg ggaaggaaat agtcttaaat cagcttataa
     4621 gctctataga aacacttcca tttatggatg ataggaagat agtaataata aaagactttg
     4681 aactattaaa aggaaagaaa aagaatttta cagatagtga tgagaaatac ttaatagagc
     4741 atttagacaa tattccagac accacaacta tagtttttgt tgtatatgga gatgtagaca
     4801 aaagaaaatc attagttaag aaaataggaa acaatggaat tgtttttgat tgtgataagc
     4861 tttctgatat ggatttgttt aaatggataa aaaaatcttt tgctttaaat gatgttataa
     4921 tagataattc tcaaatcatg tattttattg aacaagaagg atacagagat aagagtagtg
     4981 aaaagacatt atctgattta gaaaatgaaa taaataaaat aagttcattt gttgctaaag
     5041 ggaataatgt aaccaatgat gtaattgata aattatctca aaaaaaagtt gagaatgata
     5101 tattcaaatt aatcgattat attggagaac aaaatgcttc taatgcaatg aaaatattaa
     5161 atgatatgat acaagaaggc gaatctgtat taggtatatt ttctatgata gcaagacaat
     5221 ttaaaataat tatgcaagta agacagttac aacttgatgg atattcaaca aaacttatag
     5281 ctgataaatt aaaaatgcat caatttgttg taggaaaagc attaaagcaa actaaaaatt
     5341 tttctgatga tataattgtg gaaatattaa actatatatt agaaagtgat tacaaaataa
     5401 agacaggtct tataagagat actttagcag ttgaaatgtt ggttagtaga tactgtaaaa
     5461 gagaagcaat ttaaaaaatc tatatattaa taaaaaataa atagcaagat atgttatagt
     5521 tcagagtaga tgtaagctaa attaagtttg atatacaatc aactataata tgtctaacta
     5581 tttatttttt attgtataaa tttacaaaat aaaaaacccc tacatagggg ttattatata
     5641 taagaaatat aaactggtaa ttaagcgttc ataccgttta atttttgagc taatttagct
     5701 acttttctag ctgcagcatt cttatgtata gttcctttag aagctacttg gtatattctt
     5761 ttttgagcgt attggaactt agcaacagca tcctctctgt ttccagctgc aacagcatct
     5821 tcgaatcttc ttattgcagt ttttatttga gattttctaa ctctatttaa agcagttttc
     5881 ttttcgataa cacttattct tttcttagct gattttatat ttgccaccgt gttcacctcc
     5941 ctgtattttt ttatcatatt catcgtcaac atattagatt ttaacagaaa taaatctaaa
     6001 aatcaaggat tattatttat atattttaat aatgtttttt attttgattt tttagactgt
     6061 aaattgtgta taaataattt gcaatatctt gtttttaaat tggtatattt tgtgttttaa
     6121 atattttgac atatataatt aatatttcat tttgtaacaa atatttgaga taatacaaac
     6181 tattaacata cattttgata gtatttccaa taattataag ataatacaag aaattactca
     6241 tttttgtaaa tttaatgaaa aattataaaa atataaattt atatgcttaa ttaagataat
     6301 ttttccaata atcaagataa tttttttcat tagtatgtat gatagatgca tcaaacaata
     6361 taactaatat gttagaaggt tatatgtaat taatgtcagt gtttatatag aatagcattt
     6421 gtagcattta ttatagttgt aattactatt tatatcatag acttgaatag tctaaaaaaa
     6481 tagactataa tatttttata gactaaagaa agggctgata agatggacat agcaaaacta
     6541 attttaaatc ctgcaaggct tcgtatttta caatatattc gactttatgg cagtgttcgt
     6601 acatctgata ttgtaaagta tttaaatgat attcctcgtg caacagttta tcaccatgta
     6661 aaaattttag aagagaacaa tatgattgaa gtaattaaag aaaatcgtgt gcgtggtact
     6721 attgaaaagg tttatgcact aaaggaatat acaacatcta tggaaggaga gaccttcgtt
     6781 gcactttcta cagcatttta tgtaggtctg atgcaggaaa tgaatgaata ctttagcaga
     6841 aaagagcaag accataaaaa ggataatgta tttttctctt cagcattact atatatgagt
     6901 gataatgaat atgaaaatct tcttaaaagt atagctgatt tattgaaacc atatatagaa
     6961 caaaaaccta aatcaaatct aaaacttaga aaactttcaa ttatatcttc tccacctgta
     7021 gctgataatg ataaaacaaa ataacatgca acccattttt ataaaaggag gttatatatg
     7081 aaatttcatg agtttggaaa taaaaacaga ccacatatta tgttgattca tgggggaggg
     7141 aatgcatggt ggaactattt gcgacaagca gaagttttgt ctaacaaata tcatgtgata
     7201 cttcctacat tggatggtca tggagaagaa tatgaaacaa catacatttc tacagaggat
     7261 acagcagata agttgatatc atatatagat gaaaattgca gtggacatct tttcgctttg
     7321 tgtggggtat cacttggtgg tcaaattgtc atggaacttt tatctcgaaa acctaatatt
     7381 actaaaaaag caattattga tggtagtatt tgctacccaa gacctcttat ggcaaggttt
     7441 tgtattgctg gtattcgatt ttttggagga cttttgttta gtgagaaggc ttgtcgcttt
     7501 caaattgcta tgatgccaaa attgttacct gaaaatatgc aatatccaga tgaaataaaa
     7561 gcgtattata tgacagatat gccacatgta agaaaagaaa cattctataa tatgtatcgt
     7621 acttacatga tgaactatac acttaaagag aatgttaaaa aaaccacagc acaagtaatg
     7681 tactggtatg gagaaaaaga aatgaagtgt gtcaaaaaat ctgcaaaact gtttcagtct
     7741 tatgtgccct cttgtgagat ttatgaagct aagagctata atcatgggta tttatcactt
     7801 tatcttccaa atgaatggtt ggaaattgca gaaccatttt tccaaaagga ataaaggtgt
     7861 taggtgatta ttgtataatt attgctgttt ttttatatag taaataaatt aggtataaat
     7921 ataaaaaatt attttagtta atatattagt gaaagcttaa taggaaaatt ataaatagat
     7981 taaaggtagc acctatataa atagttgcta cctttttctg tttgtaaata aatgatagat
     8041 aagaaggttt actgacaaat cactgactaa acccaatttt cttacattga aagccttaat
     8101 tttacttgtt ttaaataaat ttacaatttc ttgttataaa atactaaatt ttgttaacaa
     8161 ttataattag catttaattt atactggagg tttatatgat tagtgtaaga acagatttag
     8221 ctttagaagc tagtgaaatg tgtgaaaagt ctcaagaagg aagtagtatt cctggtgtaa
     8281 aaatagaaac taaagaatta gaaaattgca ttgtaactaa agttgaagta atagacaggc
     8341 aaggcgcaga gataatgaat aaagatatag gaaagtatat aactctggaa agtaatctta
     8401 tgaagtttga tgatgatgag tcaagagaag aaatgataag ttatttaaaa gaggagttag
     8461 tagatatatt tggccaagac aaaaataaaa aaaccctggt aattggtctt ggaaatagaa
     8521 atattacttc tgatgcgtta ggaccaaaga gtgtatcaaa aacacttgta actagacatc
     8581 tttttaaaaa ttataataaa gactatgatg atgatttcac tgaggtatca gctttaagcc
     8641 caggagttat gggtgttact ggaatagaaa ctagtgaaat agtaaaatca ttggttgaga
     8701 aggttaagcc agatagagta gtagcaatag atgcattggc ttcaagaaaa atggagagag
     8761 taaactctac tatacagata tcaacagcag gaatatcacc tggtggaggt gttggcaata
     8821 ctagaaaatc tctaactaaa gaaactttag gcgtggatgt tattgcaata ggcgttccaa
     8881 cggtggttga tgcagcaact cttacaatag atgtcttaga tatggcaata gacaatttaa
     8941 tagctcaatc agaagaaaca gaaagttttt atgaaatgtt gaaaaaactt aaagaagaag
     9001 aaaaatatca tctaataaaa gattcgttag acccatatga caagaactta atagtaacac
     9061 caaaggatat agatgataca atcgaaaatc tatctataat aataagtgaa ggcttaaata
     9121 ggtctttaca tccaggtaga ttggtataaa ataaggagga gcaattatgt ttaaaaaatg
     9181 cattaaagta gtaaccttga cttttatatt agtatgcatt ttaccaggaa aatcattagc
     9241 tttgaatcaa gatgattttt taaagttttt agtaaattca tcttatccag aagctaaagt
     9301 cgaaggaaat gatacagaaa ataaaaagaa taataaaaat aaagaaacaa gtaaggaaaa
     9361 taaggaagaa agtaaagaag gaaacactaa atctaaagat gcaagtaaag ttgataataa
     9421 aaaagagagt gaaaaagaat atataaaact ttatgttggt aaagaaaatg ttccagatat
     9481 tgagtcaaaa aattcagaca caactgaaac taacacaaca tctagttcag attataagga
     9541 tgatttaaga gtaaccaaag aaaatccaag aattttgata tatcatactc atggatgtga
     9601 gacttattca aattcacctg atggaaatta tcattcaaga gataagaaaa attcagtaat
     9661 ggaagtagga agtgcattaa ctagtgcttt agatagcaaa ggatggggtg tagtccatac
     9721 tactaaatac catgactatc catcttataa taactcttat gcaagtagct taaaaacaat
     9781 acaaagtata ttacctaaat ataactcagt ggatatagcg atagacttac atagagatgc
     9841 tagggattta actaatccag ccactaagga aaaagaccat ttaaaatata caactatgat
     9901 aaatggagag agagtatcaa agttcttctt tgttgtagga ggtaaaaata caaatagaaa
     9961 acaacttaga gctttagcag aagatataac tgcttttgct gagaaaaagt atcctggtct
    10021 agtatctcca atagtagaaa aagactatgc tagattcaat cagtttgcag ttaagaatca
    10081 tatgttagta gaaataggga ataacgcaac aagtgtagaa gaatctaagg ctactacaaa
    10141 gtatttagcc gagattttgg atgaatattt taaacaaaaa aattagagct ttaagttata
    10201 attaggagca tgctaaatga gccgattaga aaaaaatata gaaagtaaaa gtaaactaag
    10261 aaaatacaag tatattatga gaatcttatt tttgattact atgattatat gtacatcagt
    10321 ttgtgtattt atagtggaca gtaatgcaaa aagtatgcta ggaatgaaac aatatgattt
    10381 tccaagtatt aaatttgata taacaaaatt tgattttgac ataccaaatt ttgatataca
    10441 tgaaatcata aaaaaattta ataagtagta ataagtgaat aaggtttatt gattaaaata
    10501 agccttattt tagttatata tttttaatgt ataaaataaa aattcattaa ctgtatttta
    10561 taggatttac tttaagttta tgatattata taatatttag tgttagactt gataatccac
    10621 atataatgtg gataattttg tttagtgcca attagtttat attcaaaaat tttaaatttg
    10681 gtatataaag agtttttaga taatgtaaaa taataactat aagaataatc aagaggaggg
    10741 aattttaagg tggacaataa acaaagtaga actagaaatt ttagtataat tgcacatata
    10801 gaccatggaa agtctacctt agctgataga ttaatacaac aaactgggct tgttagtgaa
    10861 agagacatga aaagtcaatt acttgacaat atggaccttg aaagagaaag aggaatcact
    10921 ataaagcttc aaaacataag attaatgtat aaagcgaagg atggaaatga atattattta
    10981 aatcttatag atacacctgg acatgtagac tttaactatg aggtatcaag aagtttagct
    11041 gcatgtgaag gagcactatt agtagttgat gcagctcaag gtgttgaggc acaaacttta
    11101 gccaatgttt atcttgctat agaccaagat ttagaaatat taccaataat aaataaaata
    11161 gatttaccaa gtgcaagacc agaggaagta aaaaatgaaa tagaagattt aatagggctt
    11221 gattcaagtg aagctcctct tatttcagct aaaactggtt taaacataga agatgtttta
    11281 gaagatattg taaaaaatgt tcctccacct aaaggagata atgaagctcc attaaaggca
    11341 ttaatatttg attcttacta tgatgcttac aaaggtgttg tagcttatgt tagagtattt
    11401 gaaggaactg ttaaaaaggg tatgacaata aaaatgatga ataccaataa aaagtttgag
    11461 gtaactgaag ttggtgttat ggctccaggt caaactgaac ttagtgaatt atcagcaggt
    11521 gatgttggtt atattgctgc tagtataaaa gatataagaa gttgtcgtgt tggtgataca
    11581 ataacagatt caaataatcc tacagaagaa cctttaccag gatacaaaaa agctactcca
    11641 atggtatatt gtggtatata tccaggagaa ggagaaaagt atgagaatgt tagagatgct
    11701 cttgaaaagc ttcaagtaaa tgatgctgca cttgaatatg aagctgagac ttcagcagca
    11761 ctaggatttg gttttagatg tggtttctta ggtcttttac atatggagat aatgcaagaa
    11821 agacttgaga gagaatttaa ccttgatata attactacag ctccatctgt tatatataga
    11881 gttacaaaaa tggatggaga ggttgtaatg atacaaaatc ctgcaaactt accagaacca
    11941 tctgagataa agatgataga agagcctata gtaaaaggag atataatagt tcctaaagac
    12001 tatgtgggtg ttgttatgga gctttgccag gaaagacgtg gaaatatgtt aaatatggaa
    12061 tatatagatg atagaagagt aatgcttcac tatgaccttc ctctcaatga agttgtttac
    12121 gatttctttg atgctttaaa atcaagaact agaggttatg gttctcttga ttatgaggtt
    12181 aaaggttatg tagcatctac acttgtaaaa cttgatatat taatcaataa agagcaagtg
    12241 gatgcactta gttttattgt ccatgagaca cgtgctttcc ctagaggaaa ggcaatgtgt
    12301 gagaaattaa aaggagaaat tccaagacat cagtttgcta taccaataca agctgctgtt
    12361 ggtaataagg ttattgcaag agaaacaata agcgctctaa gaaaagatgt tcttgctaaa
    12421 tgttatggtg gagatatatc tcgtaagaag aaacttcttg aaaaacaaaa agaaggtaag
    12481 aagcgtatga gacaaattgg ttctgttgaa gttcctcaaa aagcatttat gtctgtgctt
    12541 aaattagatg agtaaatcct tataaattta gataaagata tttaaaagct agagtgattt
    12601 tagaattatt tctaaattag tctagctttt ttatggtata ttttaagtaa actcatagag
    12661 aatataagta agaatattaa agaagaataa aaaggtgata tttatgatta atatagaaat
    12721 tcctaaaaaa gtagactata taattaaaga attagaaaag aatggttatg aagcttatat
    12781 agttggagga tgtgttagag attgtttatt agaaagagtt ccaaacgatt gggatataac
    12841 aactagtgct agaccagaag tggtagtaga attatttgaa aaaacaattc caactggtat
    12901 acaacatggg actgtaacag ttatgattga acatgaacca tttgaagtga cgacttatag
    12961 aattgatgga aattatagtg atggtagaca tccagattca atagaattta caaataacat
    13021 agttaaggac ttgtcaagaa gagattttac tatcaattca attgcataca attcaaagac
    13081 aggattagtc gacccattta atggctatga agatatacaa aataagtata tacgttgtgt
    13141 tggaaatcca gtagatagat ttgaagaaga tgcacttaga atgcttagag cagttagatt
    13201 ttcagcacaa ctcaatttta aaatagctga aggtactaag caaagtattc ataaaaaagc
    13261 agatttgata aaaaatgttt ctattgaaag aatccagaca gagtttaata aaatattagt
    13321 atctgattca tctaaattaa acttattaaa atcaacaggt ttgttgaaat ttataattcc
    13381 tgaaatatgt gaattagaag atgttactca acacaatcca tatcacatct atgatgtaca
    13441 aaaacatact ttaatagcta cagaagcaat tgaagatgaa ctgtatttaa aacttacaat
    13501 gttgtttcat gatttaggta aaaaagtaac taaaacaact gataaaaatg gtgttgacca
    13561 tttttatact catagtagag aatctgtaaa gattgcaaag aagattttga aaagacttaa
    13621 gtatgataat tatactatta ataaagtttt aatattaata caatatcatg attatagaat
    13681 agaacctaaa aggaagataa ttaaaaagtt attaaataaa cttgaagatg ttgaattatt
    13741 tgaagattta ataaaagtta attgggctga tacacttgcc aaaaatccaa agtatgcaaa
    13801 acaaaagata ttaaatctta ttgagtgtga aaaagaattt aaacatataa taaatcaaaa
    13861 ggagtgcttt aatcttaaag atttggctat taatggaaaa gatttgatat ctataggtat
    13921 aaaaccaggt aaagatatag gacatatttt aaataagatg cttgaaatag tcataaataa
    13981 tccagagtta aatgaaaaag aaatactaaa agaaaaagca ttaaatatat atacatttta
    14041 gattcaaata taaaaaattt aattacttta aaaaacatag aataaaatac ttatttagat
    14101 aaaataaata atttttagag aatattttag aagttacgat tgtatgattt ttaaatgcat
    14161 aggcatttac tctaggacta tagactaata taaaaaaaaa ctttgggaat actaaaacca
    14221 taaatttata aaattaatat tttatatact gtaaaacagt gaggagggac agtatgcata
    14281 ctcaactaca aaagactatt ggtctttcag cagctctttc tactgttgta ggtatggtta
    14341 taggttcggg agtttttttt aagccacaag caatatacac tacaactaat ggagctccag
    14401 gtctaggtat tatagcgtgg cttttaggtg gttttataac aataactgct gggcttacag
    14461 ctacagaaat ttcagctgct ataccaaaaa ctggtggtat gatgatttat atcgaagaaa
    14521 tatatggaga aaagttaggt tttttaactg gatggatgca aactgtatta tttttcccag
    14581 gtacatcagc agcattagga gtaatatttg ctcaacaagc atctgaactt ttaggaatga
    14641 gcccaaataa tatggcaaat gtacttccta tagcagttgg agttatacta tttttagcat
    14701 tattaaatat aataggttca tccttaggag gaaaagttca gacagtagcc actattggaa
    14761 agatgatacc tttaatttta ataatagtct ttggatttat taaaggtcaa agtagtgaag
    14821 tattaaatcc atttgtagga gatggtgtaa atgcatcaaa tgcacttgga caagtattaa
    14881 tagctacttt gtttgcatat gatggttgga taaatgttgg agccatatca ggagaaatga
    14941 agtctccaga gaaagattta ccaagagcaa tagttggtgg cttatcatta gttatggcag
    15001 tttatattat aataaatata gcttatcttt gggtagtacc agcaagtgaa ttagcaacag
    15061 ttacttcccc agctacatta gtagcaacaa gattgtttgg aaatataggt ggaaaagtaa
    15121 taactgtggg tatattaata tcagtatttg ggacattgaa tggatattta ttaacaggtt
    15181 caaggatacc ttatacatta gctgaaatgg gaactttacc agcatctaaa actcttttaa
    15241 aagtaaattc tggaggttct cctgtaaatt caatactact tataactgtg ttagcctgtg
    15301 tatatgcatt atctggtcaa tttaatcttt tgacagactt aacaatattt tcaatatggg
    15361 tattttatgt attgactttt ataggtgtta tgaggcttag aagacaaaag ccagatttac
    15421 acagaccata taaagtccct ttatatccaa taataccaat tatagctata ttaggtggac
    15481 tgtttgtaat aataaatcaa atattgactt caacagtcat atctttaggt gggatattta
    15541 taacactatt aggtttacca gtttattatt atatgaaaaa aagataacta cttaatttaa
    15601 gttagataaa tttgggtaaa ggtgaaaatt aacttttatc caa
//
LOCUS       DAELLV010000073        15621 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.073, whole genome shotgun sequence.
ACCESSION   DAELLV010000073 DAELLV010000000
VERSION     DAELLV010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15621
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.073"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     regulatory      37..138
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            321..893
                     /locus_tag="KPD58_001827"
     CDS             321..893
                     /locus_tag="KPD58_001827"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBG0265597.1"
                     /translation="MKALQEKILREGSVSGNDILKVDSFLNHQIDVAFLNEIGREFKE
                     RFKGEKVDKIFTIEASGIAIASIVSQYFDNAPVVFAKKSESKNLDKDVYETNVYSFTK
                     AREYSVKVSKKYINKGENILIVDDFLANGRAALGLKDLIEQAEANLVGVGIVIEKGFQ
                     AGGALLKANDVRLESLAVVESIDNGTVKFR"
     gene            957..1607
                     /gene="fsa"
                     /locus_tag="KPD58_001828"
     CDS             957..1607
                     /gene="fsa"
                     /locus_tag="KPD58_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBG0265598.1"
                     /translation="MRFFIDTANIEEIKEANDLGVICGVTTNPSLIAKEGRDFIEVVK
                     EISEIVDGPISAEVISLEHKGMIEEAEKLSKIHKNIVIKIPMTAEGLKAVKVLSSKGI
                     KTNVTLIFSAGQALLAARAGATYVSPFVGRLDDISQNGLDLIEEIVDIFSVNSIEAQI
                     IVASVRNPIHVLQAARMGADIATVPLKVINQMIKHPLTDKGIDSFMKDWEGASLKL"
     gene            1759..4683
                     /locus_tag="KPD58_001829"
     CDS             1759..4683
                     /locus_tag="KPD58_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0265599.1"
                     /translation="MDKILREIQREDKKNPYTDQELAEMLNVARSEVISVRKKNNILD
                     SRERRKKILIKDISKIVSENPQMSERKITEILIENGYNISRSAVSKLLKEENLNQSKI
                     GIKDKDPASSGKYVKVSKVVEEDGFEELIGIKGSLKEKVNLAKSAIMYPPNGLHTIIY
                     GETGVGKSELATCMYKYAIKNNIKEENSPFIVFNCADYAENPNLLIAQLFGVVKGAYT
                     GADANREGLVEQANNGILFLDEIHRLPAAGQEILFSLIDRGEFRRLGESSSNRKANVL
                     IISATTENPDSNLLQTFRRRIPMVINLPNMSERPKSERYEIIIKFFEREAKRVNKNFI
                     ITKEVMCALMNYKCTGNIGQLKSDIQVTCARAFSKSIFSSDKVIIDLDSLRDYIKSGY
                     YDCNCKNEKYSEFSVEDIYIDISELVSGKNRILSDNEISEIYNYAEKELKVLESKCFS
                     EEELKNAFIEKLDKKFNAIKNNKNLSDRRSRIDWGGQLKESTLEIMDKVIVFIKEQFK
                     HVNQGLYLALAIHIEHAIGRIKDGKTIINPSLDKIKASMPMEYELSRYIMGIVEDLTQ
                     VTFPEDELGYLAYYINKFCYNEESIKDKVKVVIVTHGKVGIEMSKVVNHILGIECTLG
                     IEIALTDSPSEGIEHVLEELQKIEARKGILVLIDMGSLVILGDEVEKRLGIRCKTVNR
                     VDTLLAMEAGKLATIEGKSLDGIIADLKKNKNYAMINTNKFSYRKNEYGKKNVIITLC
                     LSGVGTALNLKEHIEKQIKEYDTLIEVKPVAFLNNNLEEELNDIEAEYNIIAICGTID
                     IDYKNVPFISYNEVLGKNGINKVLEHLNSKQPALNIENQLNNLIHEDLILYNFEGISK
                     EYIIDTLVSKLEEGGYVDSKYILSVYKREAMGSVVMASKVAVPHGLPENVIKPAIAIA
                     RLNKPIVWDNKFMVDLVVLLALKENNKKEIRSLFSKINDQHTLEVLLNTEDKNEIKEL
                     LS"
     gene            5275..5733
                     /locus_tag="KPD58_001830"
     CDS             5275..5733
                     /locus_tag="KPD58_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0265600.1"
                     /translation="MEFINFVSDKVIDLKMDCKTKEDFFKEIHNKVFDLGFVKEEFGE
                     KILARENVFPTGLNLGDYGVAIPHTDAEYIKEQFISVCTFNEPVVFSSMEDQDEKVPV
                     NLAFVLGLNQPHSQLSVLTELMGIMQNKELVDKLMSSTDKEEVLKTLKSL"
     gene            5794..6069
                     /locus_tag="KPD58_001831"
     CDS             5794..6069
                     /locus_tag="KPD58_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0265601.1"
                     /translation="MKKILVACGAGIATSTVVCDKVERLVKENNIQAEVIQCKIAECA
                     TKQEGADLIVSTTILPTTYNIPAIKATAYITGVNTAALDKKILDALK"
     gene            6128..7402
                     /locus_tag="KPD58_001832"
     CDS             6128..7402
                     /locus_tag="KPD58_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012951111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBG0265602.1"
                     /translation="MDKLLAVVQYILNMGPTVILPITIVVLGMIFRIKFTKALKSGLT
                     IGIGFVGINLVVNLLTSTLGPAAQGMVERFGLNLTVIDVGWPTTASATWASPVAPILI
                     PVCLLVNLLLLYFNKTKILNVDIWNYWHFIVAGAVGYIVTGSYVWAIFCAILMELLVL
                     FIAEKTAKNVQEFYDLEGIALPTGSTASFAPLGYLVGKFVEKIPGINKIQADPDSIQK
                     RFGIFGEPMMMGIILGIILGALAGYDVAGIFNVGISMGAVMLLMPRMVRILMEGLIPI
                     SESIRDFLQAKYGDRELYIGLDAAVATGHPAVISTALILVPITLFLAVILPGNKVLPF
                     GDLATIPFYMAFIVCFRKGNIVQSVITGTIVIAISLYMATNFADVHTQLLQQANMMPE
                     GTTMVTSIDTGGNLLKWGILKLSQLVSSFGIF"
     gene            7532..8584
                     /locus_tag="KPD58_001833"
     CDS             7532..8584
                     /locus_tag="KPD58_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBG0265603.1"
                     /translation="MKAAVLHGTNDMRFEDIEIKPCESDEVKIKVMAAGICGSDPPRV
                     LKHWKYPVPAIPGHEFSGVIAEVGKDVKNVKVGDRVVAIPFIPCNECEYCKRGLFSLC
                     DDHGMLGAKSFGAFAEYVNIKATNVLPIGDMDFEDAAMIEPLAVAMHGVLNIGVQVGD
                     TVAVMGSGTMGQLVIQGLKIAGAGTIIAVDISDNKLRESKELGADIIINAKDINPVEK
                     IKELTGGKGVDIALECAGSKITQEQCLLITKKKSKIGFLGIAYSDITLSEEAFENIFR
                     KELELKGFWNSYSAPFPGQEWTKGINLVNEGKIKLKEMVSHRFSLEDTYKAFEMIRDR
                     KEEFNKILILPQGVEK"
     gene            8584..9615
                     /locus_tag="KPD58_001834"
     CDS             8584..9615
                     /locus_tag="KPD58_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBG0265604.1"
                     /translation="MKAVVKTKPGYDNVEVLEVEEPKATGDKVKIKVEYSGICGSDIH
                     SFKGEYANIKAPVTLGHEFSGIVVEVGEDVKNIKVGDRVTSETTFATCEKCIYCETKD
                     YNLCPTRKGIGTQVNGSFAEYVLSREESIHVLPENVSLLSAALTEPLACCVHAALEKT
                     TIESSDTVLIIGPGPIGLLLAQVVKSQGATVIMSGVTKDKERLEIAKNLGVDRTVNVM
                     EESLADVVNEMTDGNGVNKGFDCSGFMPAVNEALRLLRKKGTFVQVGIFAKKLNEMDQ
                     EAIIQRELQYIGSRSQKPSSWITALDLMASGKVNTEALVTRTVGLDEFKEGITALMNG
                     EEIKVAVKS"
     gene            9707..10519
                     /locus_tag="KPD58_001835"
     CDS             9707..10519
                     /locus_tag="KPD58_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBG0265605.1"
                     /translation="MSTLRDHSNNIRKNIIKMVAEAKSGHPGGSLSIADMLTVLYFDK
                     MNVCAENPKMADRDRFVLSKGHAAPALYATLAEKGFFPEEELITLRKFGSKLQGHPDM
                     KKVAGIDMSTGSLGQGLSAANGMALAGKLDNKDYTVYTILGDGEIQEGQIWEAAMTAS
                     HYKLDNLIAFVDLNGLQIDGSNEEVMNVNPVDDKFKSFGWNVIVINGHSFEEIGNAVD
                     EAKKVTGKPTVIIAKTVKGKGVSFMENNAAWHGTAPNAEQAEQALKDLEGGL"
     gene            10522..11442
                     /locus_tag="KPD58_001836"
     CDS             10522..11442
                     /locus_tag="KPD58_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HBG0265606.1"
                     /translation="MGIATREAYGQVLKELAENKDIVVLDADLGKATKSISFKEVAPD
                     RFFDMGIAEGDMIGTAAGLATCGKIPFASTFAIFAAGRGYEQIRNSVAYPNLNVKIAA
                     THAGVTVGEDGGSHQAIEDISLMRGIPNMVVLNPADALEARQAILASIDYNGPVYIRL
                     GRAATPDVNSENYKFEIGKGTVLREGSDITVIATGIMVAKALEAAEELAKEGINVEVV
                     NISTIKPLDEALIKETAKKTGKVVTAEEHSIIGGLGSAVCEALAETKDVVVRRIGVKD
                     VFGQSGTPADLLKHYGLTTEDIVKNIKELL"
     gene            11617..12063
                     /gene="rpiB"
                     /locus_tag="KPD58_001837"
     CDS             11617..12063
                     /gene="rpiB"
                     /locus_tag="KPD58_001837"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HBG0265607.1"
                     /translation="MKISIGCDHGGYELKEYIKENLINKGIEVEDVGTNSKESVDFPI
                     YAKKVSQNVQSKKSDLGILCCGTGIGMSIAANKFKGIRAAVVSDCFSAKATREHNNTN
                     VLCLGERVIGKGLAMRIVEEWLNSSYNGERHARRLQMIDDIEKENL"
     gene            12093..12758
                     /locus_tag="KPD58_001838"
     CDS             12093..12758
                     /locus_tag="KPD58_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBG0265608.1"
                     /translation="MSIICASIMCADQMKLKEELEALEEAGVKLLHCDVMDGIFVKNL
                     AMGPELLKSISENTTIPLDIHLATETPDKYIDMMSYIKPKYISFHVESSTNVKADIQK
                     LRNYGIGPVLAISPQTSVDKIEEYISLVEGILVMTVNPGFAGQKFNLSVLDKLDKLTE
                     ILKDYDNPPFIEVDGNINKDTIKLMNGKKVDIYVVGTSALFNDKPPISYKDKIEELKE
                     SIK"
     gene            12868..14370
                     /locus_tag="KPD58_001839"
     CDS             12868..14370
                     /locus_tag="KPD58_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HBG0265609.1"
                     /translation="MGDLYKLQNGTDIRGIAYENDSKEVNLTVEEVKKIAKAFHIWLK
                     EKTKKDKVTVAIGTDSRITGSQFRSTVIETLTNDDCNVIDCEIATTPAMFMTTIMDGY
                     NSDGAIMITASHLPYYYNGLKFFTESGGLEKTDIKEMLDIAVKNDSVDYEEVNKKGEV
                     IVKNLIEDYSNLLIDKIRKGVNSSKNYEKPFSGLKILVDAGNGAGGFFAEKVLHILGA
                     DTTGSQFLNPDGMFPNHIPNPENKEAMESICKAVLDNKSDLGIIFDTDVDRAAIVGKN
                     GKPINKNALIAVISSIVLEEHPKTAIVTDSITSEGLAKFINELQGRHHRFKRGYKNVI
                     NEAIRLNSEGEECHLAIETSGHAALKENYFLDDGAYLIAKILIKVAKLSLEGKTIEEL
                     IENLEEAQEEKEVRIGINKEDFRPYAEDILKDLDSHVKSIDGWSVAPNNFEGIRVNCD
                     ESNGDGWFLLRISLHEPLLALNIESNKIGGAEQIYSKLKLFLEKYDLKGL"
     gene            complement(14682..>15621)
                     /locus_tag="KPD58_001840"
     CDS             complement(14682..>15621)
                     /locus_tag="KPD58_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG0265610.1"
                     /translation="MYIYYAEAIAIVILFTLVNTVFHFYIYNFLSVKFNYINNISKNI
                     KNIENGNLKYKLKVIGDDEISTLAKSINNITGGLETAVDANIKNERMKAELITNVSHD
                     LKTPLTSILSYVDMLKNNDFDRETINDYINILDKKSQRLKLLIDDIFEASKLSSGDVE
                     FNITKTDIRELLIQSIVEFEDKIQNSSLDFIIETPEYAVFTMIDGKKTWRVFENLICN
                     ILKYSMPNTRVYIDMFIKEENIILTFKNISNDKLNLKPEELIERFRRGDISRKTDGSG
                     LGLSIAQNIIELENAHMEIIIDADLFKVMLTFRRVN"
BASE COUNT         6264 a         1652 c         2912 g         4793 t
ORIGIN      
        1 gttgaataat agagaattat aatatataat taaaaggaat aaattaatat atttacctgt
       61 atatgtttga taatatggtt caaatgtttc tactaagcta ccattaatag cttgactaca
      121 ggagtgaaga agatatagtt tatacttatt ttaaattata tattaagtta attactttga
      181 attttaagct cctgtctatg atatagacag gagctttttt agtattttaa tataaaatac
      241 ttacaagaat agatagcaaa attagaacaa atataatttt tttattaata taattttaga
      301 aagggctagg tggaagttca atgaaggctt tacaagaaaa gatattgaga gaaggtagtg
      361 tttcaggaaa tgatatacta aaagttgata gttttttaaa ccatcaaatt gatgtagctt
      421 ttttaaatga aataggaaga gaatttaaag agagatttaa aggagaaaag gtggataaga
      481 tatttacaat agaggcttca ggaatagcta tagcatcaat tgtttcacag tattttgata
      541 atgcacctgt agtatttgca aaaaaatcag aatctaaaaa tttggataaa gatgtatatg
      601 aaactaatgt atattcattt actaaggcaa gagagtattc agttaaagtt tctaaaaagt
      661 acataaacaa aggcgaaaat atattgatag tggatgattt tttggcaaat ggaagagctg
      721 ctcttggact taaagattta attgagcaag cagaagctaa cttagttgga gtaggaattg
      781 tcattgaaaa aggatttcaa gctggaggag cacttttaaa ggctaatgat gtaagattag
      841 agtctttggc agtagttgaa tctatagata atggaactgt aaaatttagg taatttatga
      901 tattatatat ttgtgtttag cacatatttt aactatgtaa ttatggagga ttttttatga
      961 gattttttat agacacagcc aatattgaag aaataaaaga agcaaatgat ttaggtgtaa
     1021 tctgtggtgt aactactaat ccgtctctaa tagctaaaga aggtagagat tttatagagg
     1081 tagttaaaga aataagtgaa atagtagatg gaccaataag tgcagaagta ataagtttag
     1141 aacataaagg tatgatagag gaagcagaaa aattatctaa gatacataaa aatatagtta
     1201 taaagatacc tatgacagca gaaggtctaa aagcggtgaa agtactatca agtaaaggta
     1261 taaagactaa tgtaacgctt atattctctg ctggacaagc tcttttagct gctagagctg
     1321 gagctacata tgtaagtcct tttgttggta gattggatga tatatcacaa aatggtcttg
     1381 atttaattga agaaatcgtt gatatattta gtgtaaatag tattgaggct caaataatag
     1441 ttgctagtgt tagaaatcca atacatgttt tacaagctgc tagaatggga gcagatatag
     1501 caacagttcc tcttaaagtt ataaaccaaa tgattaagca tcctttgaca gacaaaggaa
     1561 tagatagctt tatgaaggat tgggaaggtg cttctttaaa attataatta taaactacgc
     1621 tttataagta tattaaagag gttattatgg aaattctaag taataaggct aggctatttt
     1681 gcctagtctt attttggtgt ataatatatt gaaacatatt attgaaatag attaattttt
     1741 taagtgagga ggcgaaaaat ggataaaata ttaagagaga tacaaagaga agataaaaaa
     1801 aatccatata ctgaccaaga acttgctgaa atgttaaatg tagctaggtc tgaagtgata
     1861 tcagttagga aaaaaaataa tattttagat tcgagagaaa gaagaaaaaa aatattaata
     1921 aaagatatat caaaaattgt aagtgaaaat cctcaaatgt ctgaaagaaa aataactgaa
     1981 atattaatag aaaatggata taatatatcg agaagtgcag tttcaaaatt attaaaagag
     2041 gaaaatttaa atcagtcaaa gattgggatt aaggataaag acccagcatc atcaggaaag
     2101 tatgtgaagg tgagtaaagt tgtagaagaa gatggatttg aagaattgat aggtataaag
     2161 ggaagtttga aagaaaaggt aaatttagct aagtcagcaa taatgtatcc tcctaatggc
     2221 cttcatacta taatatatgg agaaacagga gtaggtaaaa gtgaattagc tacatgtatg
     2281 tataaatatg ctataaaaaa caacattaaa gaagagaaca gcccatttat agtgtttaac
     2341 tgtgcagact atgcagaaaa tcctaatcta ttgatagctc aactctttgg ggtagtaaaa
     2401 ggtgcatata caggagcaga tgcaaataga gaaggtcttg tagaacaagc taataatgga
     2461 atattgtttt tagatgaaat tcatagattg cctgctgctg ggcaagaaat cttattttca
     2521 ttaatagata gaggagaatt tagaaggtta ggagaaagta gttctaatag aaaggcaaat
     2581 gttctcataa taagtgctac aacagaaaat ccagattcta atttacttca aacatttaga
     2641 agacgtatac caatggtaat aaatttacct aatatgagtg aaagaccaaa atctgaaaga
     2701 tatgaaataa taataaagtt ttttgaaaga gaagcaaagc gagtaaacaa gaattttata
     2761 ataactaaag aagttatgtg tgcacttatg aattataagt gtactggaaa tataggtcag
     2821 ttaaagagtg atatacaagt aacttgtgct agagctttta gtaaatcgat attttctagt
     2881 gataaagtaa ttatagattt agatagttta agggattata taaaaagtgg atattatgat
     2941 tgtaattgta aaaatgaaaa gtattctgag ttttcagttg aggatatata tatagatatt
     3001 agtgaattgg tgagtggaaa aaatagaatt ttatctgata atgagataag cgagatatat
     3061 aattatgcag aaaaagagct taaggttttg gaaagtaagt gcttttcaga agaagagtta
     3121 aaaaatgcat ttatagaaaa gttagataag aagtttaatg ctattaaaaa caataaaaat
     3181 ttatctgata gaagaagtcg aatagactgg ggaggacaat taaaagagtc tacactggag
     3241 ataatggata aggtaattgt atttattaag gagcagttta agcatgtaaa tcaagggctg
     3301 tacttggctc tagcaattca tattgagcat gctataggta ggataaaaga tggaaaaact
     3361 ataataaatc cgagtttaga taaaataaaa gcatcaatgc caatggaata tgagttgtca
     3421 agatatataa tgggtatagt agaagatttg acacaagtaa catttccaga agatgagctt
     3481 ggatatttgg cttattatat aaataaattt tgttataatg aggaaagtat aaaagataaa
     3541 gttaaagtag taatagttac gcatggtaaa gttggaattg aaatgtctaa agttgtaaat
     3601 catattttag gtatagaatg tacactaggt atagaaatag cacttacaga ttcaccatca
     3661 gaggggatag agcatgtatt agaagagctt caaaagatag aagctagaaa aggtattcta
     3721 gttctaattg atatgggttc tttggtaatt cttggtgatg aggttgaaaa aaggcttgga
     3781 attagatgta aaactgtaaa tagagtagat acattattgg caatggaagc aggaaaactt
     3841 gcaaccattg aagggaaaag tttagatggt ataatagctg atttaaagaa aaataagaat
     3901 tatgctatga taaatacaaa taaatttagt tacagaaaaa acgagtatgg aaagaaaaat
     3961 gtaatcatta ctttatgttt gagtggtgta ggtacagctt taaatttaaa agagcatata
     4021 gaaaaacaaa ttaaggaata tgacacttta attgaagtaa agccagttgc ttttttaaat
     4081 aataatttag aagaagagtt aaatgatatt gaagctgaat ataatattat tgctatctgt
     4141 ggaactattg atatagatta taaaaacgtg ccttttattt cttataatga ggtacttggt
     4201 aaaaatggta ttaataaagt attagagcat ttaaatagta aacaaccagc tttaaatatt
     4261 gaaaaccagt tgaataattt aatacatgaa gatttgattt tatacaattt tgaaggaatt
     4321 tcaaaggagt atataattga tactcttgtt tcaaaacttg aagaaggtgg atatgtcgac
     4381 tcaaagtata tattaagtgt atataaaaga gaagcaatgg gaagtgttgt aatggcctct
     4441 aaggtagccg tgccacatgg acttccagaa aacgttataa agcctgcaat agcaattgcg
     4501 aggttgaata aacctatagt atgggacaat aaatttatgg tggatttggt ggtactactg
     4561 gcacttaaag aaaataataa gaaagaaata cgaagcttat tttcaaagat taatgaccag
     4621 catactttag aagttttatt aaatactgaa gataaaaatg aaataaaaga acttttaagc
     4681 taattgttag taccgagaag aaagacaaaa aaatttgctt tagtagtata aaatttaaaa
     4741 ctactcaatt gctaagtagt taattactct atataaggct agttctatga aatttattta
     4801 tatgatttta tagaattagc ttttttcttg gtaaataaaa tatatgaatt tatttatgta
     4861 aaaagtaaat tttattggac aattctataa aaatttttcc tatatttctt ataaaatata
     4921 gaatatataa aaaatatata aaaataaatt aaattcataa taaataattt tataaaatag
     4981 ttgttaaaca taaaaaacca tttgtaaaaa acaaaaaaat catttctcta acaaaaaaaa
     5041 ccacaaaaaa tataaaaaag tatggctagt taagtgatta acaagtaaaa attaaaataa
     5101 aaatcaaaaa aaatttatca atgaaagtca gctaaaatcc tggaagagag cattattttt
     5161 gggaaaacat tttcttttgt ttcacaaaaa aagccacttt ttggcacaca acatgcttta
     5221 tataaagtca agaaaaacta aggtataaga caaaggcaat taggaggtgt aaatatggag
     5281 tttataaatt ttgtaagtga taaagttata gaccttaaaa tggattgtaa aacgaaagaa
     5341 gatttcttta aagaaataca taataaagtt tttgatttag gctttgtaaa agaagaattt
     5401 ggagaaaaaa tattagcaag agaaaatgta tttccaacag gtttaaattt aggtgattat
     5461 ggagtagcaa taccacatac agatgcggaa tatattaaag agcaatttat atcagtctgt
     5521 acttttaatg aaccagtagt atttagttct atggaagacc aagatgaaaa agtacctgta
     5581 aatttggctt tcgtattagg acttaatcaa ccacacagtc aattatctgt attaacagaa
     5641 ttaatgggaa ttatgcaaaa taaagagtta gttgataagt tgatgagttc tactgataaa
     5701 gaagaagttt taaagacact aaagtcttta taaaaataaa aaagtactaa aaataaaaaa
     5761 tataaaaata aaaattatta ggaggaaata aaaatgaaaa aaatattagt agcatgtgga
     5821 gcaggaatag caacatcaac agtagtttgt gacaaggtag agagattagt taaggaaaat
     5881 aatatacaag cagaagttat acaatgcaaa atagctgaat gtgcaacaaa gcaagaagga
     5941 gcagatttaa tagtatcaac aactatacta ccaacaactt ataatatacc agcaataaaa
     6001 gcaacagcat atataactgg agtaaataca gcagcattag ataaaaaaat attagatgct
     6061 ttaaaataat cctatcatag atacattgaa ataatatttt ataaaacaga aaaaggagga
     6121 tttataaatg gataaattat tagcagtagt acaatacatt ttaaatatgg gaccgacagt
     6181 aatactacca ataacaattg tagtactagg aatgatattt agaattaagt ttacaaaagc
     6241 tttaaagtca ggtcttacaa ttggtatagg atttgtgggt ataaacttag ttgtaaacct
     6301 attaacatca acactagggc cagcggctca aggaatggtt gaaagattcg gacttaattt
     6361 aacagtaata gatgttggtt ggccaacaac agcatcagca acatgggctt caccagttgc
     6421 accaatatta ataccagttt gtttattagt aaacttacta ttattatact tcaataaaac
     6481 aaagatatta aacgttgata tatggaatta ttggcacttt atagtagcag gtgcagttgg
     6541 atatatagta acaggaagtt atgtttgggc tatattctgt gcaatactaa tggagttatt
     6601 agtactgttt attgcagaaa agacagcaaa aaatgttcag gagttttatg atttagaagg
     6661 tatagcatta ccaactggtt caacagcttc atttgctcca cttggatatt tagtaggtaa
     6721 atttgttgaa aaaataccag gaataaataa aatacaagca gacccagact ctatacaaaa
     6781 aagatttggt atatttggag aaccaatgat gatggggatt atactaggta taatacttgg
     6841 agcacttgcg ggatatgatg tagcaggaat attcaatgta ggtatatcaa tgggtgcagt
     6901 aatgttactt atgcctagaa tggttagaat attaatggaa ggtttaattc caatttcaga
     6961 atcaataaga gatttcttac aagcaaaata tggagacaga gaactttata taggtctgga
     7021 tgcagcagta gcaactggac atccagcagt tatatcaaca gctcttatac ttgttcctat
     7081 aactttgttc ttagcagtta tactaccagg aaacaaagtt ttaccatttg gagacttagc
     7141 gacaatacca ttttatatgg catttatagt gtgttttaga aaaggtaata tagttcaatc
     7201 agttataaca ggaacaatag taatagctat atcactttat atggctacaa actttgcaga
     7261 tgttcataca caattattac aacaagcaaa tatgatgcca gaaggaacaa caatggttac
     7321 aagtatagat acaggtggaa acttgctaaa atggggaata ttgaaattat ctcaattagt
     7381 ttcttcattt ggaatattct agtatgtagt taatagagat tactcttttt tagtaaagta
     7441 tgcgaaaatt taacatttag cagctactaa aaaagagtaa tattttttat aaatttttaa
     7501 aaatacaaaa tacaagagga ggagatttat cgtgaaagca gcagtattac atggcacaaa
     7561 tgatatgaga tttgaagata tagaaataaa accatgtgaa agtgatgaag taaaaataaa
     7621 agttatggca gcaggaatat gtgggtcaga tcctccaaga gtattaaaac attggaaata
     7681 tccagttcca gctataccag gacatgagtt ttctggagta attgctgaag ttggtaagga
     7741 tgtaaaaaat gtaaaagttg gagatagagt agttgcaatt ccttttatac cttgtaatga
     7801 atgtgaatat tgtaaaagag gattattttc attgtgtgat gaccatggaa tgttaggagc
     7861 taaaagtttt ggagcttttg cagagtatgt aaatataaaa gctacaaatg tacttccaat
     7921 aggagatatg gactttgaag atgctgcaat gatagagcca ctagcagttg ctatgcatgg
     7981 agtacttaat ataggtgttc aagttggaga cactgtagcc gtaatgggta gtggaacaat
     8041 ggggcagttg gtaatacaag gacttaaaat agcaggggca ggaacaatta tagcagttga
     8101 tatatctgat aataaattaa gagaatcaaa ggaattaggg gctgatataa taattaatgc
     8161 taaagatatt aacccagtag aaaaaataaa agaacttact ggtggaaaag gtgttgacat
     8221 agctcttgaa tgtgcaggtt caaaaataac tcaagaacaa tgtttactta taactaaaaa
     8281 gaaaagtaag ataggatttt tagggatagc atattcagat ataactttat cagaagaagc
     8341 atttgaaaat atatttagaa aagaactaga attaaaaggt ttttggaact cttattctgc
     8401 accatttcca ggacaagaat ggactaaggg aatcaatcta gttaatgaag gaaagataaa
     8461 attaaaagaa atggtatctc atagattctc acttgaagat acttacaaag catttgaaat
     8521 gataagagat agaaaagaag aatttaacaa aatattaatt ttaccacaag gggttgaaaa
     8581 ataatgaaag cagtagttaa aactaaacca ggctatgata atgtagaagt tttagaagta
     8641 gaagaaccga aagctacagg agacaaggtt aaaataaaag tagaatatag tggtatatgt
     8701 ggttcagata tacattcatt taaaggtgaa tatgcaaata taaaagcccc agtaacatta
     8761 ggtcatgaat tttctggaat tgttgtagaa gtaggagaag atgttaaaaa tataaaagta
     8821 ggagatagag ttacatcaga gactactttt gcaacttgtg agaaatgtat atattgtgaa
     8881 actaaagatt ataacttatg cccaactaga aaaggtattg gtactcaagt aaatggaagt
     8941 tttgctgagt atgtattatc aagagaagaa agcatacatg tacttcctga aaatgtatct
     9001 ttattatcag cagcccttac agagccacta gcttgttgtg ttcatgcagc tcttgaaaag
     9061 actactatag aaagttctga tacagtttta ataataggac caggaccaat tggtttatta
     9121 ttagctcaag ttgttaaatc tcaaggtgct acagttataa tgtctggtgt tacaaaagat
     9181 aaggaaagat tagaaatagc taaaaatctt ggagtagata gaactgtaaa tgtaatggaa
     9241 gaaagtttgg cagatgtagt gaatgaaatg actgatggaa atggagtaaa caaaggattt
     9301 gattgttctg ggtttatgcc agcagttaac gaagccttaa gactgcttag aaaaaaaggt
     9361 actttcgtac aagttggaat ctttgctaaa aaattaaatg aaatggacca agaagcaata
     9421 atccaaagag agttacaata tataggaagt agatctcaaa agccaagttc ttggattaca
     9481 gcattagatt taatggcaag tgggaaagta aatacagaag ctttagttac tagaactgtt
     9541 ggtttagacg aatttaaaga aggtattaca gctttaatga acggggaaga aataaaagta
     9601 gcagtaaaat cttaaaaaaa catttgtatt attttaaaaa aaattgtaaa atctactgta
     9661 ggataaaaaa atgtaaggtc ttaaaaaata acaggaggaa gaaagtatga gcactcttag
     9721 agatcattct aataatatta gaaaaaacat aataaaaatg gttgcagaag ctaaaagcgg
     9781 acatccaggt ggttcattat caatagctga tatgttgaca gtattatatt ttgataagat
     9841 gaacgtatgt gcagaaaatc caaaaatggc tgatagagat agatttgttc tatcaaaagg
     9901 tcatgcagca ccagctttat atgctacatt agcagaaaaa ggatttttcc cagaagaaga
     9961 attaataaca ctaagaaaat ttggttctaa gttacaaggt cacccagaca tgaaaaaagt
    10021 agcaggaatc gatatgtcta ctggttctct tggtcaaggg ctatctgcag caaatggtat
    10081 ggcacttgca ggaaaattag ataacaaaga ctatacagta tatactatat taggtgatgg
    10141 agaaatccaa gaaggtcaaa tatgggaagc tgcaatgaca gcatcacatt acaaattaga
    10201 taatttaatt gcatttgttg acttaaatgg attacaaata gatggttcta atgaagaagt
    10261 aatgaatgta aatccagtag atgacaaatt caaatctttt ggatggaatg taatagtaat
    10321 aaatggacat tcttttgaag aaataggaaa tgcagttgat gaagctaaaa aagttacagg
    10381 aaaaccaact gtaataatag ctaaaacagt aaaaggtaaa ggtgtttcat ttatggaaaa
    10441 taatgctgca tggcatggaa cagctccaaa tgcagaacaa gcagagcaag cattgaaaga
    10501 tttagaagga ggtctatagg aatgggaata gcaactagag aagcttacgg acaagtgtta
    10561 aaagaacttg cagaaaataa agatatagta gtattagatg cagatttagg aaaagctaca
    10621 aaaagtatat catttaaaga agttgcccca gatagatttt ttgacatggg gatagcagaa
    10681 ggtgacatga ttggtacagc agcaggtctt gctacttgtg gaaaaatacc ttttgcgagt
    10741 acttttgcaa tatttgcagc aggaagaggc tatgagcaaa taagaaactc tgtagcatat
    10801 ccaaatttaa atgtaaaaat agcagctact catgcaggtg taacagtagg tgaagatggt
    10861 ggtagccatc aagctataga ggatatatct ctaatgagag gtataccaaa tatggttgta
    10921 ttaaatccag cagatgcttt agaagcaaga caagctattc ttgcatctat tgattataat
    10981 ggacctgttt atataagatt aggtagagct gcaactcctg atgtaaacag tgaaaattat
    11041 aaatttgaaa taggaaaagg aactgtttta agagaaggtt ctgacataac agtaatagct
    11101 acaggaataa tggtagcaaa agccttagaa gcagcagaag aacttgctaa agaaggtata
    11161 aatgtagaag ttgttaatat atctacaata aaaccattag atgaagcact aataaaagaa
    11221 actgctaaga aaactggaaa agtagttaca gcagaggagc acagtataat aggtggttta
    11281 ggttcagctg tatgtgaagc tttagcagaa actaaagatg tagtagttag aagaattggt
    11341 gttaaagatg tatttggtca atcaggtaca ccagctgatt tattaaaaca ctatggttta
    11401 actacagaag atatagtaaa aaatataaaa gagttgttat agatataaat atatctaaat
    11461 aacaaattga tttgacataa gtcaattaaa gctgtcttaa aaatagaaag aaatttctac
    11521 aatgggacag ctttattttt acaatacttt atttttatga taattataaa atagtctgaa
    11581 agtaatcaaa aaaattataa aaagaggtgc tatttaatga aaatatctat tggatgtgac
    11641 catggtggtt atgaattaaa ggaatatata aaggaaaatt taataaataa aggtatagaa
    11701 gttgaagatg tgggtacaaa ttcaaaagaa agtgtagatt ttccaatata tgcaaaaaag
    11761 gtaagtcaaa atgttcaaag taaaaaatct gatttaggca tactttgttg tggaacagga
    11821 ataggtatgt ctattgcagc aaataagttt aaaggaatca gagcagctgt tgtttctgac
    11881 tgtttttcag ctaaggcaac tagagaacac aataatacaa atgtactttg tttaggggaa
    11941 agagttattg ggaaaggatt agcaatgaga attgttgaag aatggttaaa ttcaagttat
    12001 aatggagaaa gacatgcaag aagattacaa atgattgatg atatagaaaa agaaaattta
    12061 taaatatata atttataaca agggggagcg ttatgtctat tatttgtgct tcaataatgt
    12121 gtgcagacca aatgaagtta aaagaggaat tagaagctct tgaagaagca ggtgtaaaat
    12181 tacttcattg tgatgttatg gatggaatat ttgttaaaaa cttggctatg ggaccagagt
    12241 tattaaaatc aataagtgaa aatacaacaa tacctttaga catacactta gccacagaaa
    12301 ctccagataa atatattgat atgatgtctt atataaagcc aaaatatatt tcttttcatg
    12361 tagaatcaag tacaaatgta aaagctgata tacaaaagtt aagaaattat ggtatagggc
    12421 cagtgttagc aataagccct caaacaagtg tggataaaat agaagaatat ataagtttag
    12481 ttgagggtat cttagtaatg acagtaaatc cagggtttgc aggtcaaaag tttaatcttt
    12541 ctgtattgga taagttggat aaacttacag agatactaaa agattatgat aatcctccat
    12601 ttatagaggt tgatgggaat ataaataagg atactattaa acttatgaat ggtaaaaaag
    12661 tagatatata tgttgttggt acttcagctt tatttaatga taagcctcca atttcatata
    12721 aagataaaat agaagaacta aaagaaagta taaagtaacc atgtaataaa attataatat
    12781 gtttctgttt aatagtaaaa atttaggtgg aaacaagaaa ttttataata tttaataaaa
    12841 attttggttg agtaaggaga attttttatg ggagacctat ataaactaca aaatggtact
    12901 gatattagag gaatagcgta tgaaaatgat tctaaagaag ttaacctaac tgtagaagaa
    12961 gtaaaaaaaa tagctaaagc gtttcatatt tggttaaaag aaaagactaa gaaagataaa
    13021 gtaactgttg ccataggaac tgattctagg ataacaggaa gccaatttag aagtactgtt
    13081 attgaaactt taactaatga cgattgtaat gtaattgatt gtgagattgc cacaactcca
    13141 gctatgttta tgacaactat tatggatgga tacaattctg atggggcgat tatgattaca
    13201 gcaagtcact taccttatta ctataatggt ttaaagtttt tcacagaaag tggaggttta
    13261 gaaaaaactg atataaaaga gatgttggat atagctgtta aaaatgatag tgtagactat
    13321 gaggaagtaa ataaaaaagg tgaagttata gtaaaaaatt taatagaaga ttattctaat
    13381 ttattgatag ataaaattag aaaaggtgta aattcaagta aaaattatga aaagcctttt
    13441 tctgggctta agatattggt tgatgcagga aatggggctg gtggattctt tgctgaaaaa
    13501 gttttacaca tactgggagc agatacgaca ggtagtcagt ttttgaaccc agatggtatg
    13561 ttcccaaatc atatacctaa tcccgaaaac aaagaagcaa tggaaagcat atgtaaagct
    13621 gtgttggata ataagagcga tctaggaata atctttgata cagatgtaga cagagctgct
    13681 attgtaggta aaaatggcaa accaataaat aaaaatgctc ttatagctgt tatatcatca
    13741 atagtattag aagaacatcc aaaaacagct atagtaacag attcaattac ttctgaagga
    13801 cttgctaaat ttataaatga attacaagga agacatcata gatttaaaag aggatataaa
    13861 aatgtaataa atgaagctat aagattgaat agtgaaggtg aagagtgcca tttagctata
    13921 gaaacgtctg gacatgctgc tttaaaagaa aattatttct tagatgatgg ggcatattta
    13981 atagctaaaa ttttgataaa agtagcaaag ctatccttgg aaggaaagac tatagaagaa
    14041 ttgatagaaa accttgaaga agctcaagaa gagaaagaag ttagaatagg cattaataaa
    14101 gaagatttta gaccatatgc agaggatata ttaaaagatt tagattctca tgttaagagt
    14161 attgatggat ggtctgtagc accaaataat tttgaaggca taagagttaa ttgtgatgaa
    14221 tcaaatggag atggatggtt tttacttaga atatcactac atgaaccatt attagcttta
    14281 aatattgaat caaataaaat tggtggagca gaacaaattt attctaagtt aaaacttttc
    14341 ttagaaaaat atgatttaaa aggtctttag ggtaaataga aagaatatat ttatattgaa
    14401 gaataaatgt aaatttgtac ctaagattac aaatattaca aaattgtaat cttaggtaac
    14461 agagatgtaa ctgattatgg aaacactatg tgatattata gatacagaga gatgaaatat
    14521 atgatgaatt atagagaaat ataatttaaa atgtattaat atcacaccaa aaaggtataa
    14581 tcaaaaaata tgtaaatata aaccccaaca tttagaaaaa gacctctaag gaggtttttt
    14641 tcttgtttaa ataacttaaa atatattttt atattaatat atcaattaac tcttctaaaa
    14701 gtaagcatta ccttaaataa atcagcatct ataattattt ccatgtgtgc attttctaat
    14761 tcaattatat tttgagcaat tgataaacca agaccagagc catctgtttt tctagatata
    14821 tcaccccttc taaatctttc tattaactct tctggcttta gatttagttt atcatttgaa
    14881 atatttttaa acgttaatat tatattttct tcctttataa acatatctat gtagacccta
    14941 gtgttaggca tagagtattt tagtatattg caaattagat tctcaaaaac tctccaagtt
    15001 tttttaccat ctatcatggt aaaaacagca tattcaggtg tttctattat aaaatctaaa
    15061 ctgctatttt gaattttgtc ttcaaactct acaatacttt gaattaaaag ttctcttata
    15121 tcggtttttg ttatattaaa ttctacatct ccactactta gcttagatgc ttcaaatatg
    15181 tcatctatta ataattttaa tctttgtgat tttttatcta aaatatttat atagtcattt
    15241 atagtctctc tgtcaaagtc attatttttt aacatatcaa catagcttaa tatagatgta
    15301 agtggagttt ttaagtcatg agatacgttt gtgataagct ctgccttcat cctttcattt
    15361 tttatattag catctacagc agtttcaaga ccgcctgtta tattatttat acttttggct
    15421 aaagtagata tttcatcatc tcctataact tttaatttat actttaaatt gccattttct
    15481 atatttttta tattttttga tatgttattt atgtaattaa acttaacaga taaaaagttg
    15541 tatatataaa aatgaaaaac tgtgtttaca agtgtaaata aaattactat agctattgcc
    15601 tcagcataat atatatacat a
//
LOCUS       DAELLV010000074        15619 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.074, whole genome shotgun sequence.
ACCESSION   DAELLV010000074 DAELLV010000000
VERSION     DAELLV010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15619
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.074"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     regulatory      123..206
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            786..1463
                     /locus_tag="KPD58_001841"
     CDS             786..1463
                     /locus_tag="KPD58_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0265611.1"
                     /translation="MYNLLVVDDDVDILNINQIYFSNIGFNVYTSETAKDAMDIVESI
                     VLDCIILDISLPDIDGYKMCNDIKEKVNIPIIFLSNYEHEEEKVRGFLAGGDDYITKP
                     YSLKELELRIYARMRQYKNITSVPNVLKFSALVINVNSRKVTFDGESIDLTTMEFEIL
                     LFLAENKEQVFSKIEIYNQVWKMPDIGDQHTVQVHIAQMRKKINSLSREHQYIQTVWG
                     KGYKFVP"
     gene            1535..3550
                     /locus_tag="KPD58_001842"
     CDS             1535..3550
                     /locus_tag="KPD58_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBG0265612.1"
                     /translation="MKKYNILKGTLIVLIVSLLAITGLNLLYKYDNKYTYKSIRASNG
                     ILTIDNDTFTKGQLVFLIDDWEFYNHKIFKPNDFTEQDIKPEYVFIGQYPDFSMRKGN
                     QSPYGNATYRMRIKNEGKDKLLSLELPEIFSASEVWINGEMVSKLGDVGTEKYRPKIK
                     NSVVSFIAKKNTEIIINVSNFSHYYSGLYYPPALGETKDISNMMFYRLLFYSIICFTT
                     FAIAIFSLSVWFLSNKSKIYLYFGCMCVFFAIHVSYPFVHLLGLPLVSFTYAVEDLSY
                     FMVVLCIICINGSISKIEENKVYRFLILPLGITMSIISIVIPIILLPYDMYFVNIYGK
                     LIDIYKYIVFIYILMSSLVSIYKKTCSEHISSYILVSVNTVFGISILFDAVTSNRFEP
                     IFTGWQTEYCGFVIVILFSVMMINRNRIIIKENEKLTRNLEHEVEVRTNELTTLLNER
                     KRFLADVAHDLKAPVSAIQAFIDLIKVGNIHVDEETRTYLYAINQKSNEVQNRVRSLQ
                     EFTSQDKSIGISEKICLNNFIKEIYENNLPDTQACGVNFEAYVLEKKIYAKINKEMLT
                     RAFENLIYNALTFTPFDGKIILSMDKEDGFAIIKISDNGKGIPPENIPKVFDRFFTER
                     DNNEPKGQGLGLYIVKSIIREHGGEIFVDSELGKGTVFTIKLSLCFL"
     gene            3600..4280
                     /locus_tag="KPD58_001843"
     CDS             3600..4280
                     /locus_tag="KPD58_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0265613.1"
                     /translation="MQTKILIIDGDKDNCQKLKGFLEEKGISIDLAYNCEEAIGKIFS
                     NKYDLIFLEIILSDGDGWTLCKKIRNVTTCPIVYMTYINEDQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRMNSYVNNNENNTKLEEYNRTSGVIKLEDKIIKLTPTENKLL
                     NFFIENPEKTLTTKEIYEHVWMNEYLEDNYSVVVAVNGLRKKIEKDYKNPQKIITIRG
                     AGYYFNKE"
     gene            4797..5243
                     /locus_tag="KPD58_001844"
     CDS             4797..5243
                     /locus_tag="KPD58_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265614.1"
                     /translation="MAISDLAFGTECLKTSFQMLFSVISLVNINLLQAQILIAIGGLI
                     SILAMVSLTLFLSAKCKDSLTSMQIAFSICLIPMGIGVSWIGYLLPPGGIGFKISLLY
                     QMTSFNFLHLGGMSFWTPHIILFFSVIEIPIFIFLAIRTYCKHQVS"
     gene            5624..6541
                     /gene="pstC"
                     /locus_tag="KPD58_001845"
     CDS             5624..6541
                     /gene="pstC"
                     /locus_tag="KPD58_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HBG0265615.1"
                     /translation="MIVKSRLVGLEKNDNNGNKTKYVIEKVAKNIFLLSALVAVASLL
                     LIIGFVFYKGLRPFIFEGYSFIDFLTGADWVPSANKFGISSMIVASIFATLGALVIGV
                     PVGILTSVFIAEVAPKRLAKIMSPAVELLAGIPSVLYGVFGLAVIVPTIQSIFNLPKG
                     QSLLAIVIVLAVMMLPTIISVSETAIRAVPKAYKEGSLALGASKIETIFKVVLPAAKS
                     GVLAAVVLGVGRALGETMAVILVAGNSPVMPTAITDSVRPLTTNIALEMGYAFGTHQE
                     MLFATGVVLFTFILILNLVLNKLSNKAVN"
     gene            6543..7367
                     /gene="pstA"
                     /locus_tag="KPD58_001846"
     CDS             6543..7367
                     /gene="pstA"
                     /locus_tag="KPD58_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBG0265616.1"
                     /translation="MRKFKENLLKSLVYLSALFTIVSLVVIVGFIFIKGIGNMNFNFL
                     FSNYSASGDGGILPMIITTLYTVVVSIVVATPIGILAAIYLQEYAKKGKAVTAIRFAT
                     ESLSGIPSIVYGLFGGIFFVVTLKMGYSIVAGSLTVAIIILPVIIRTTEEALKTVPQA
                     YRESSLALGATKFQTLYKVVLPSAIPGILSGVILSVGRIIGESAAILLTAGTVAKMPG
                     GIFDSARTLTVHSYLLTKESGDIATAASIGIVLIVIVLALNMLARFVAKKLNKANY"
     gene            7386..8150
                     /gene="pstB"
                     /locus_tag="KPD58_001847"
     CDS             7386..8150
                     /gene="pstB"
                     /locus_tag="KPD58_001847"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBG0265617.1"
                     /translation="MELIDKIKMSVKDLDLFYGDKQALKKINMDIKENKVTALIGPSG
                     CGKSTFIRTLNRMNDLIEDVTIKGNISVDGEDIYTSDDVINLRTKVGMVFQKPNPFPM
                     SIYDNVAYGPRTHGLRDKKQLDKIVEESLKGAAIWDEVKDRLKSSALGLSGGQQQRIC
                     IARAIAMRPEVILMDEPTSALDPISTLKVEELIEDLKKDYTIVIVTHNMQQAARISDE
                     TAFFLNGEVIEFSDTKTMFTTPVDKRTEDYITGRFG"
     gene            8178..8831
                     /gene="phoU"
                     /locus_tag="KPD58_001848"
     CDS             8178..8831
                     /gene="phoU"
                     /locus_tag="KPD58_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBG0265618.1"
                     /translation="MVNNSLDISIDTLKEQTVLMMEKCEEAIEKSVTCMIEKDLEGAR
                     KVVEDDEEINKLREEIRDKSIELIVLKQPMARDLRFIYSLGNISIELERIGDYAANIA
                     REMLKIGGEAYIKELIDIPKMAKECKSMMSQTREALILFDASIAYDVGTKDDIVDGLY
                     EQVQIDCLKIMHENSNTIDQGVKLLLIGRYLERIGDHITNVCEKIIFAIEGKMVEIG"
     gene            8989..9858
                     /locus_tag="KPD58_001849"
     CDS             8989..9858
                     /locus_tag="KPD58_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBG0265619.1"
                     /translation="MKLQKMGMRTIKTGISVTLCMILGDYLVENMFYSLVACIISVQD
                     TVKGSLKTGINRVEGTILGGIIGFIFAIIKAGDPFLCGLGVMCSIYLCNLFKIKSVVV
                     ACVTFLAIHLGVGNSDPVYYSLHRVLDTSVGVLFGVLVNYYVARPNYLNSVIDEFKKI
                     EELSMLLIEDKILRKENLNVEKFEEEVEKLEEIYSKLIDELDYSLSETNIEKIENALR
                     ICREIHFHMQSIELLENKLYLKEHTYKYLKKTYNVDKLDWNIDEEKSPVFNYHLMKIM
                     IEIELLRKLNKSY"
     gene            9945..11381
                     /locus_tag="KPD58_001850"
     CDS             9945..11381
                     /locus_tag="KPD58_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase"
                     /protein_id="HBG0265620.1"
                     /translation="MNTRNDNSKILEIYKMKLFGELVKAVWNDDIEDIENLPNKILNE
                     DLKDNLNNYFSGKFDKDTMVNLIRVTMGLDVTEDTDKNLKESLHEAVNLDEVKRPIIS
                     VISDACKYCDENNKEDESCKIRSKHINCNEEDVCSACGDCVSRCKLGAISDKIEFIPM
                     IKLLKDIKSPVYAIVAPAFVGQFGKDVSPGKLRTALKKIGFEDMIEVALAADMLTAKE
                     AYDYYEHMKKDEEGCFITSCCCPIWVSLIQNSFPQILENVSPSVSPMIACGRAVKILN
                     PDAKVVFIGPCMAKKKEAMLEDIKDAVDFVLTFKELQEIFDALELNPAIMDESNRVEA
                     SLSGRVYARTGGVSKAVKLSVRRIDKNIKFEEKAFEGTKECMDGLKKVLNKEIEATFI
                     EGMGCVGGCVGGPKRILSVEEGTKNVDEYSESTNMETPFDNLNVLQFLTSLGIKRVES
                     LGEKEEEKVKKIFSRDIKSNKKENKYKK"
     gene            11429..12256
                     /locus_tag="KPD58_001851"
     CDS             11429..12256
                     /locus_tag="KPD58_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG0265621.1"
                     /translation="MKIKDNKAVFHVFIRIFLIAVLILTGTLLEEKINNVPVENISSK
                     GENTLELLSQSNTLGEIIYKASQEETKGPGATSEKVAYITIDDGPSKYTNALLDILKE
                     NDVKATFFMLNQNMKNHKEEVRRVLEEENSIGFHSVSHDIHKLYKSPEVTVGEFNTCN
                     NTLQEITGQSSKLIRLPYGSKPYMPEGSYNKLMANDYLIWDWNLDTQDWKSSTSQIVS
                     NILYYGRKRDELVVLIHEKEQSLNALNNIIRILKERGYTILPITENITPKNFWQENL"
     misc_binding    12547..12809
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            12885..15551
                     /locus_tag="KPD58_001852"
     CDS             12885..15551
                     /locus_tag="KPD58_001852"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBG0265622.1"
                     /translation="MENTNLSKTYNPKDFEARLYKKWMDEGYFKSKPNPDKKPFTIMM
                     PPPNITGQLHMGHALDHTLQDILIRWKRMDGYEAFWLPGTDHASIATEVKVVERIKKQ
                     EGKTKYEIGREEFLKRAWEWKDEFGGKISNQLKQLGDSCDWDKERFTMDEGCNEAVIE
                     FFVSLYEKGHIYRGNRIINWCPDCKTTLSDAEVEHEEHDGNFYHIKYPLKDSEDFLEI
                     ATTRPETMIGDTGIAVNPEDDRYKHLIGKTAILPLVGRELPIVADSYVDLEFGTGAVK
                     MTPAHDPNDFEVGLRHNLEQLNTMNEDGTMNEVCGKYEGMDRFECRKAIVADLKEQGY
                     LIKIKEHNHNVGTCYRCHTVVEPRLSEQWFVKMEELAKPAIDILKKGELEFVPDKFDK
                     TYLQWLENIRDWCISRQLWWGHQIPAYYCQECGEIVVARKMPDKCPKCGSTHFKQDED
                     ALDTWFSSALWPFSTLGWPNKTEALDYYYPTSVLVTGYDIIFFWVVRMAFAGMFCMNE
                     KPFDHVLVHGLVRDSQGRKMSKSLGNGIDPLEIIEQYGADALRFTLTTGNSPGNDMRF
                     YMERVEFARNFANKLWNASRFVFMNIDEDIIKNMTRESVKEDLTLADKWIISRANNIV
                     KEATNNMDKFDLGIALQKIYDFTWSEYCDWYIEMVKPRLYGEDANAKSAALYTLTYVL
                     EKILKLLHPYMPFITEEIYTHLPTVEGCIIVSEWPKYNEEDNMAEEEDMMNLLMEGIR
                     SIRNVRAEMNVPPSKKAKLIIIPSEEKIEAIELGKDYFITLASASNVEIAKDKSNVPE
                     DAVGVVIDGVEIFIPLNELVDFEKEIERLSKEKKKLEGEIKRVNGKLANQGFLAKAPE
                     SLIEEEKAKKEKFEEMIKSVEERLTNLESKIK"
BASE COUNT         6199 a         1624 c         2815 g         4981 t
ORIGIN      
        1 ctttagataa aataggtatt ttagtatctt atatctaaga atatggaaat attgacaaaa
       61 taatattaca atgttagaat aaaaaagaaa tttatgaaaa tatataaaag atttttaata
      121 aaaatatttt agaaactgag aagtatatct tattattggg catctggaga tatatggagt
      181 tagtggtgca accggctatg aatataattt ttatattgca tagtcttttt ttgttttaaa
      241 attaacaaaa atgttgcaaa atttgaaaat aagagtatta gtcgttaaga tttttattga
      301 taggtaaaat tttggctttt aaagtagcca tttgttggaa aagggaaatt tttttaaaag
      361 aataatagta ttataataag aaaataaagt ttttataaac attgagatgc agaatataaa
      421 ccttttgtaa caatatattt accaaaataa tacaatttat tttatattat aaccatataa
      481 atcatataaa tcaatactca aactagccaa tagacaagtt tctagaaaaa taataaattt
      541 atacgtgaaa atgactttaa gctccattct ccaacaatcg gagactttaa ttagtattta
      601 ataatgaatc ttaatgttta atacaatatt caagaattat tcaaaaatac ttaagtatta
      661 tagtctagtt tgtaaagata ttataaaata ataataccag ttatatatga aaataaaaca
      721 ttataatttg taataactat aaaacttgga attataatta aaataccttt gaatggagtg
      781 gtgtaatgta taatttactg gttgtagatg atgatgttga tattttaaac ataaatcaaa
      841 tttatttttc taatataggt tttaatgtat atacatcaga gactgctaaa gatgctatgg
      901 atatagtaga gagtatagtt ttagactgta taattttgga tatttcattg ccagatatag
      961 atggatataa aatgtgcaat gatataaaag aaaaggttaa catacctata atatttttat
     1021 ctaattatga gcatgaagaa gaaaaagttc ggggattttt agctgggggg gatgattata
     1081 taacaaaacc ttatagttta aaagaacttg aacttagaat atatgctcgt atgaggcagt
     1141 acaaaaatat tacatcagtt cctaatgtgt taaagttttc agcgttagta ataaatgtaa
     1201 attcaagaaa agtaactttt gatggagaaa gtatagattt aactactatg gaatttgaaa
     1261 tattattgtt tctagctgag aacaaagaac aagtattttc aaagatagag atatataacc
     1321 aagtttggaa aatgcctgat attggagatc aacatacagt gcaggttcat attgcacaga
     1381 tgagaaaaaa aattaattct ttaagcaggg agcatcaata cattcaaacc gtatggggaa
     1441 aaggatataa gtttgtacca taagtgagat actgtaatta ataaatagtt cttgttttat
     1501 ttaatacgac tatgaaagag atgtggttga tgcaatgaaa aagtataata tactaaaagg
     1561 aactctcatt gtattgatag tctctctgtt ggcaataaca ggattaaatc ttctgtataa
     1621 atatgataat aaatatacat ataagtctat tagagcaagt aatggaattt taactataga
     1681 taatgatact ttcactaaag ggcaacttgt ctttttgata gatgactggg agttttataa
     1741 tcataaaata tttaaaccga atgattttac agagcaagat ataaaaccag aatatgtttt
     1801 tataggtcaa tatccagatt tttctatgag aaaagggaat cagtcaccat atgggaatgc
     1861 tacttaccgt atgagaataa aaaatgaagg caaggataag ctactaagtt tagagcttcc
     1921 tgagattttt tccgcaagtg aagtatggat aaatggagaa atggtttcta aattaggaga
     1981 tgtaggaaca gaaaaatata gacccaaaat taaaaattca gtagtaagtt ttattgcaaa
     2041 gaaaaataca gaaattataa tcaatgtatc gaatttttca cattattata gtggtttgta
     2101 ttatccgcct gcattaggag aaacaaagga cataagtaat atgatgtttt atcgattact
     2161 tttttatagt ataatttgct tcacaacttt tgcaatagct atattttcat tgtctgtatg
     2221 gtttctttca aacaaaagta aaatttactt atattttgga tgtatgtgtg ttttctttgc
     2281 catccatgta tcctatccct ttgtacattt gttaggactg cctttagtaa gctttacata
     2341 tgcggttgaa gatttgtcat attttatggt agttttatgt attatttgta taaatggaag
     2401 tatatccaaa atagaagaaa ataaagtgta tagattttta attttaccat taggaattac
     2461 aatgagtatc atatctatag tgataccaat tattcttctt ccatatgata tgtactttgt
     2521 caacatatat gggaagttaa tagacatata taaatatatt gtatttatat atatattaat
     2581 gtcttcgctg gtttcaatat ataaaaagac atgctctgag cacataagtt cttatatttt
     2641 agtttcagta aacactgttt ttggaattag catattattt gatgcagtta cgagtaatag
     2701 atttgagcct atatttacag ggtggcaaac agaatattgt gggtttgtaa ttgttatttt
     2761 attttctgtt atgatgataa acaggaatag aataataata aaagagaatg aaaaacttac
     2821 aaggaatctt gaacatgaag ttgaagtgag aactaatgaa cttacaacat tgttaaatga
     2881 acgtaaaaga ttcttggcag atgtagcaca tgatttaaag gcacctgttt cggctataca
     2941 agcatttata gatttgataa aggttggtaa tattcatgta gatgaagaga caagaaccta
     3001 tctttatgct ataaatcaaa agtcaaatga agtacaaaat agagtacgta gtttgcaaga
     3061 atttacatca caagataaaa gcattggtat tagtgaaaaa atatgtctga ataattttat
     3121 taaagagata tatgaaaata atttgccaga tacacaagca tgtggtgtaa actttgaagc
     3181 ttacgtattg gagaaaaaaa tatatgcaaa gataaataaa gagatgctta caagagcatt
     3241 cgagaattta atttataatg ctttgacctt tactccattt gatggaaaga taattttatc
     3301 aatggataaa gaagatggat ttgcaattat taaaatttct gataatggaa aaggtatacc
     3361 tccagaaaat ataccaaagg tatttgacag attctttaca gaaagagaca ataatgagcc
     3421 aaaagggcaa gggttagggc tatatattgt caaatccatt ataagagagc atggtggaga
     3481 aatatttgta gattcagaac taggaaaagg aactgttttt acaataaaat taagtctatg
     3541 ttttttataa taatagctta tttaatataa aagaataatg atatttggga gtgattataa
     3601 tgcaaactaa gatattgata atagatggag acaaggataa ttgccagaag ctaaaaggat
     3661 ttctggagga aaaaggaata agtatagatt tagcatataa ttgtgaagaa gctattggaa
     3721 aaatatttag caataaatat gatttaatat ttttagaaat aattctaagt gatggtgatg
     3781 gatggactct ttgtaaaaaa attagaaatg taactacttg tccaattgtt tatatgactt
     3841 atataaatga agaccaaagc attttaaatg ctttaaattc tggtggagac gattacttaa
     3901 taaagcctct aaatttagaa atattatatg caaaagtaaa agctatttta agaagaatga
     3961 actcatatgt aaataataat gaaaataata caaaattaga ggaatataat agaactagtg
     4021 gagtaataaa gttagaagat aaaataataa aattgactcc aacagaaaat aagttgttaa
     4081 acttttttat agagaatcct gaaaagactt tgactactaa agagatatat gaacatgtat
     4141 ggatgaatga atacttagag gataactata gtgtggtagt tgcagtgaat ggattaagaa
     4201 agaaaataga gaaagattat aaaaatcctc agaaaattat aacaataaga ggagcaggat
     4261 attattttaa taaggaataa taaatatatt aatatcatta ataattgtta taaatgtgaa
     4321 atgctaacga tatctaaact aaattattat aaaatagctg actgtaattt aattgataaa
     4381 gcctattctt ctagactttg ccagttaaat tacagtcagt tttattatat aaaaaaatgc
     4441 gtaacggaac tattatttta tagattatat ttgaaaagat aaataaattg tatatctaaa
     4501 tttatatatt attttttacc tttaatatat attaaataat aaaatagtta aataataaga
     4561 ttaagaattt cttaataaat ccagaagatg ctttcgatta tataatattt tatattttta
     4621 tattattaat tatatatgtt gcaattgctg cacctacttt ttctggtgag tatcagacac
     4681 aatcagatag catccttcgt tgtacgaaac atggacatat tcgtctagcg tttactaaaa
     4741 tattagctct attttctata tttataataa tgtttgttat atgtatttct atccatttgg
     4801 caatttctga tttagctttt ggtactgaat gtttgaaaac ctcatttcaa atgttgtttt
     4861 ctgtcattag tctagtaaat ataaatttac tacaagcaca gattcttata gctattggtg
     4921 gtctaatttc aatacttgca atggttagtt taacattatt tttatctgca aaatgtaagg
     4981 attctcttac ttctatgcag attgcttttt ctatttgctt aattcctatg ggaataggtg
     5041 ttagttggat tggataccta ttaccaccag ggggaattgg ttttaagatt agtttattat
     5101 atcaaatgac atcatttaat ttcttacact taggaggtat gagtttttgg acaccacaca
     5161 ttatactgtt tttctcagtg atagaaatac ctatttttat atttttggct attcgtacct
     5221 attgcaaaca tcaagtatcg taacaatttg atggaaataa tagtgatata aactagagga
     5281 tacaataatt atatatgtag tataaaagtt aaatttagtt aacaaatctt aatagcagat
     5341 ataggtaata aattatacat aaactgtatt attatctata tctgctattt taatatagag
     5401 aaacaacagt atattgtaaa gtaaatgtca aatatagttt taaaagtaaa attaatgtta
     5461 aaaagtaaat ataattttac acttgcttaa cttaatccta atatagattt aacttaaaaa
     5521 aagtagaata aaagtatagg aaatttgatg aagacgaatt ataaaaaatt aaattttgaa
     5581 tacagaacga aaatcgataa gtatatttta atgggagggt attatgatag taaaatctag
     5641 attagtaggt ttagaaaaaa atgacaataa tggaaataag acaaaatacg taattgaaaa
     5701 agtagcaaaa aatatttttc tacttagtgc tttagtagca gttgcaagtt tacttttgat
     5761 aataggattc gtattttata aaggattaag accatttata tttgaaggat attcatttat
     5821 agacttttta actggtgcag attgggttcc ttctgccaat aaatttggaa tatcatctat
     5881 gatagtagca tcaatatttg caacacttgg agcacttgtt ataggagtac ctgttggtat
     5941 attaacctct gtatttatag cagaagtagc acctaaaaga ttagcaaaaa taatgtcacc
     6001 agcagttgaa cttttagcag gtataccttc agtattatat ggtgtatttg gtcttgcagt
     6061 gatagttcca actatacaaa gtatatttaa tcttccaaaa ggtcaaagtt tgttagcaat
     6121 agtaatagta ttagcagtaa tgatgttacc aacaataata tctgtatcag aaacagcaat
     6181 aagagcagta ccaaaagcat acaaagaagg ttcattagca cttggtgcat caaaaataga
     6241 aacaatattc aaagttgtat tacctgctgc aaaatctgga gtattagccg cagtagtgtt
     6301 aggagtagga agagctttag gtgaaacaat ggcagtaata cttgttgcag gcaactcacc
     6361 agttatgcca actgcaataa cagatagtgt aagaccactt acaactaata tagcacttga
     6421 aatgggatac gcatttggta cacatcaaga aatgctattt gcaactggag tagtattgtt
     6481 tacatttata ttaattttaa acttagtttt aaataaactt tcaaataagg cggtaaatta
     6541 agatgagaaa gttcaaagag aatctattga agtcattagt atatctatca gcactattta
     6601 caatagtatc attagtagtt atagtgggat ttatatttat aaagggaatt ggcaacatga
     6661 attttaattt cttatttagc aattattcag catcaggtga tggtggtata cttccaatga
     6721 taattacaac attatataca gtagtagttt ctatagttgt agcaactcct ataggaatat
     6781 tggctgctat atatttacaa gaatatgcta aaaaaggaaa agcagttaca gctataagat
     6841 ttgcaactga aagtttatca gggataccat ctatagtata tggattattt ggaggaattt
     6901 tctttgtagt tacattaaaa atgggatatt ccatagttgc aggttcactt acagttgcaa
     6961 taataatatt accagtaata attcgtacaa cagaagaagc tttaaaaaca gttcctcaag
     7021 cttatagaga atcatcattg gcattaggag ctacaaaatt tcaaacttta tataaggtag
     7081 tacttccaag tgcaatacca ggaattttat ctggagtaat cttatcagtt ggtcgtataa
     7141 ttggagaatc agctgcaata ttacttacag caggtactgt agccaagatg ccaggtggaa
     7201 tatttgatag tgcaagaaca ttaactgtac actcatactt actgactaaa gagagtggag
     7261 atatagcaac agcagccagt ataggaattg ttttaatagt aattgtgtta gcactaaaca
     7321 tgctagcaag atttgttgct aagaaattaa ataaagctaa ttattaaatt taagaggtgt
     7381 gaataatgga attaatcgat aagataaaaa tgagtgtaaa agatttggat ttattttatg
     7441 gtgataagca agcattaaag aaaataaata tggatataaa agagaataag gttactgctt
     7501 taataggtcc atctggatgc ggaaaatcaa cttttataag aacgttaaat agaatgaatg
     7561 acttaatcga agatgtaaca attaaaggaa acatttctgt tgatggagag gacatatata
     7621 cctcagatga tgttataaat cttcgtacta aagtaggaat ggtatttcaa aagccaaatc
     7681 catttcctat gagtatatat gataatgtgg catatggtcc tcgtacacat ggactaagag
     7741 ataagaagca attagataaa attgtagaag aaagcttaaa aggtgcagct atttgggatg
     7801 aggttaaaga caggcttaaa tcatctgcac taggactttc tggtggtcaa caacagcgta
     7861 tatgtatagc aagagcaatt gctatgagac cagaagttat tcttatggat gaacctacat
     7921 ctgctttaga cccaatttca acattaaaag tagaggaact tatagaggat ttaaaaaaag
     7981 actacacaat agtcatagtt acacataata tgcaacaagc tgctcgtata tcagatgaaa
     8041 cagctttttt cttaaatgga gaggttatag aatttagcga tacaaaaact atgtttacca
     8101 ctcctgtaga taaaagaaca gaagactata taacaggtag atttggttaa aatatataac
     8161 tataaaggag ttagacaatg gtcaataata gcttagatat aagtattgat actttaaaag
     8221 aacaaacagt gcttatgatg gaaaaatgtg aagaagcaat agaaaagtct gtgacttgta
     8281 tgatagagaa ggatttagag ggtgcaagaa aagtagtaga agatgatgaa gaaataaata
     8341 aattaagaga agaaataaga gataagagta tagagctaat agtattaaag caaccaatgg
     8401 caagggactt aagatttatt tattcattag gtaatatatc tatagagctg gaaagaatag
     8461 gagattatgc agcaaatata gctagagaaa tgttgaagat aggtggagag gcttatataa
     8521 aagagctaat agatattcct aagatggcaa aagaatgtaa gtctatgatg tctcagacta
     8581 gagaagcttt aatcttattt gatgcaagta tagcttatga tgttggaact aaagatgata
     8641 ttgtagatgg attgtatgaa caagtacaaa ttgattgttt aaaaataatg catgaaaact
     8701 caaataccat agaccaaggg gtaaaacttc tacttatagg aagatacctt gaaagaattg
     8761 gagaccatat aacaaatgta tgtgagaaga taatttttgc aattgagggt aaaatggttg
     8821 agataggata aaaaactgct ttaaagcagt ttttttattg tctaaattaa gaaagaattg
     8881 ataaaaaaat atgattaatg ggtaaattta aaaaattgtg atatactagg aacatattaa
     8941 atatgaggat gcgtatttta tataaggtgc tttttaggag gtggaacttt gaaacttcaa
     9001 aagatgggta tgaggactat aaaaacagga attagtgtca ctttatgtat gattcttggt
     9061 gactatttag tagagaatat gttttattct ctggtagcat gtataatttc tgttcaagat
     9121 acagtaaaag gttctttaaa gacgggaatc aatagagtgg aaggtactat tttaggtggt
     9181 ataataggat tcatatttgc catcattaaa gctggagacc catttttatg tggtctagga
     9241 gtaatgtgct caatatattt atgcaatctg tttaaaataa agtcagtagt agttgcctgt
     9301 gtaacctttc tggctataca tctaggtgta ggaaatagtg accctgtata ttattcttta
     9361 catagggtgt tagatacatc tgtgggtgtt ttgtttggag tacttgttaa ttattatgtt
     9421 gctagaccaa attatctaaa tagtgttata gatgagttta aaaagataga agaactttca
     9481 atgttgctta ttgaagataa aattcttaga aaagaaaatt taaatgtaga gaaatttgaa
     9541 gaagaagtag aaaaattaga agaaatatat tcaaagctta ttgatgagtt ggattatagt
     9601 ttgagtgaaa ctaatataga aaaaattgag aatgccttaa ggatatgtag agaaatacat
     9661 tttcatatgc agtctataga gttacttgaa aataaacttt atttgaaaga gcatacatat
     9721 aaatacctta agaaaacata taatgtggat aagctagact ggaatataga tgaagaaaaa
     9781 agccctgtat tcaactatca tctaatgaaa ataatgattg aaatagagtt gttgagaaag
     9841 ctaaataaat catattaata atagtagtta aataataaat aaaaaatagt atctttgtaa
     9901 atattaaaca tatataagtt taatttatgg aggtactatt ttttatgaat actagaaatg
     9961 acaattctaa aatactagaa atatataaaa tgaagctttt tggcgaactt gtaaaagcag
    10021 tgtggaatga tgatatagag gatattgaaa acttacctaa taaaatttta aatgaagatt
    10081 taaaggataa tttaaataat tattttagtg gaaaatttga taaagatact atggtaaatt
    10141 taattagggt aactatggga ttggatgtga cagaagatac agataaaaat ttaaaagagt
    10201 cactacatga agctgtaaat ttagatgaag tcaaaagacc aataatatct gtaatatcag
    10261 atgcttgcaa atattgtgat gaaaataata aggaagatga gtcatgtaag ataagaagta
    10321 agcatattaa ttgtaatgag gaagatgtat gtagtgcttg tggtgattgt gtatcaagat
    10381 gtaagttggg tgcaatttca gataaaatag aatttatacc aatgataaaa ttgttaaaag
    10441 atataaagag ccctgtatat gcaatagttg caccagcttt tgtaggacaa tttgggaaag
    10501 atgtaagccc tggtaaatta agaactgcat taaaaaagat aggatttgaa gatatgatag
    10561 aagttgcttt ggcagcagat atgcttactg caaaagaagc atatgattat tatgagcata
    10621 tgaaaaagga cgaagaagga tgctttataa ctagttgttg ctgtcctatt tgggttagtt
    10681 tgatacaaaa tagttttcct cagatattgg aaaatgtatc accatccgtt tctcctatga
    10741 ttgcttgtgg gagagctgta aaaattttaa atccagatgc taaagttgta ttcataggtc
    10801 cttgtatggc aaaaaagaaa gaggcaatgt tagaagatat aaaagatgca gttgattttg
    10861 tgcttacatt taaagagttg caagaaatat ttgatgcatt agagttaaat ccagcaataa
    10921 tggatgagag taatagagta gaggcgtccc tttcaggtag agtttatgct agaacaggag
    10981 gtgtaagtaa agcagttaag ttatcagtta gaagaattga caaaaatata aaatttgaag
    11041 aaaaggcatt tgaaggaaca aaagagtgca tggacggtct aaagaaagtt ctaaataaag
    11101 aaatagaagc tacatttata gaaggaatgg ggtgtgtagg aggctgtgta ggaggtccga
    11161 agcgtatttt aagtgtggaa gaaggtacta agaatgttga tgaatatagt gaaagtacga
    11221 atatggaaac tccatttgat aatttaaatg tattacaatt tttaacttct ttgggaatca
    11281 aaagagtaga atctttagga gaaaaagaag aagaaaaggt taaaaaaata tttagtagag
    11341 atataaaaag taataagaaa gagaataaat ataaaaaata actttttaag tcttaaactt
    11401 atgcttttat aaaagggaga tatgggggat gaaaattaaa gataataagg ctgtttttca
    11461 tgtatttata aggatatttc ttatagctgt tttaatttta actggaacat tattggagga
    11521 aaaaattaat aatgttccag ttgaaaatat aagttcaaaa ggtgaaaata cactggaact
    11581 gctttctcaa agtaatactt taggagaaat catatacaaa gcatctcaag aagaaacaaa
    11641 aggtccaggg gctacttctg aaaaagtagc atacataaca atagatgatg gtccatcaaa
    11701 atatacaaat gcactacttg atattttaaa agaaaatgat gtcaaagcga catttttcat
    11761 gcttaatcag aatatgaaaa accacaagga agaagtaagg agagtcttgg aagaagaaaa
    11821 ctcaataggt tttcatagtg taagtcatga tatacataag ttatataaaa gcccagaagt
    11881 aacagttggg gaatttaata catgcaataa tactctacaa gaaataactg gtcagagttc
    11941 aaaattaata agattaccat acggaagtaa accatatatg ccagaaggtt catataataa
    12001 acttatggca aatgattatt taatttggga ctggaatttg gatacacagg attggaagtc
    12061 ttctacaagt cagatagtaa gtaatatatt atattatgga agaaaaagag atgaattagt
    12121 agtattaatt catgagaaag agcaaagttt aaatgcttta aataatatta taagaatctt
    12181 aaaagagaga ggatatacaa tattgccaat tacagaaaat ataacaccta aaaatttctg
    12241 gcaagaaaac ttataataaa tttaaatata gtaaaagata aagtaaatta tttataaaat
    12301 aaaacatata atataaaaaa tacgaataag aggtatactg taaggataat ataaaattaa
    12361 tgagaaagca ttaaaattaa aataatcttg caattattga cattataaga cagaataact
    12421 gtaaaaacat attttaaaat ataacaacta tataaaagag aatagacata attaagaaaa
    12481 ttcagaaaaa ataaaataaa aataattgac aaagagaata atatttgtat ataataatat
    12541 gtaatattaa agactgagaa gaagaggagt aagtaaatac tactaaaaga gaggaaaatt
    12601 cactggctgt aagattttct aagtataaga tttactgaat gtagctttgg agtttctaaa
    12661 ctgaactata agtaagttta gacggttaga accgttaaat tctttgagag tcaaagttga
    12721 taaaataaca ttaaccaata atttaattat atgttaatgt attttaagct ttggaattaa
    12781 ggtggtaacg cgagcttttc gtccttttta aagaggatga agagctcttt tttatttcta
    12841 aagatattta aaaaatttat ttataaaata caggaggcag gacaatggaa aatacgaatt
    12901 tatcaaaaac ttataaccca aaagattttg aggctaggct atataaaaaa tggatggatg
    12961 aagggtattt taaatctaaa ccaaatccag ataaaaaacc atttacaata atgatgccac
    13021 caccaaatat aacagggcaa ttgcatatgg gacacgctct tgaccataca cttcaagata
    13081 tattaatcag atggaaacgt atggatggat atgaagcttt ttggcttcca ggaactgacc
    13141 atgcttctat agcaacagaa gttaaagttg ttgaaagaat aaaaaaacaa gaaggcaaga
    13201 caaagtatga aataggtaga gaagaatttt taaagagagc ctgggaatgg aaagatgaat
    13261 ttggaggaaa aatatctaat cagcttaaac aactaggtga ttcttgtgat tgggataaag
    13321 aaagatttac tatggatgaa gggtgtaatg aagcagttat agaatttttt gtaagcctat
    13381 atgaaaaagg tcacatatac agaggaaata gaataataaa ctggtgccca gattgcaaaa
    13441 ctactttatc agatgcagaa gttgaacatg aagaacacga cggaaacttt tatcatataa
    13501 agtacccact aaaagatagt gaagactttt tagaaatagc tacaactaga cctgagacaa
    13561 tgataggaga tactggtata gcagtaaatc cagaagatga tagatataaa catttaatag
    13621 gaaaaacagc aatacttcca ttagtaggaa gagaacttcc aatagttgca gatagttatg
    13681 ttgatttaga gtttggaact ggtgcagtta agatgactcc agcacatgac cctaatgatt
    13741 ttgaagtagg attaagacat aatcttgagc agttaaatac tatgaatgaa gatggaacta
    13801 tgaatgaagt ttgtggaaaa tatgaaggta tggatagatt tgagtgtaga aaagctatcg
    13861 tagcagactt aaaagaacaa ggttacctaa ttaagataaa agagcataat cataatgtag
    13921 gaacttgtta tagatgccat acagtagttg agcctagatt atcagagcaa tggtttgtta
    13981 agatggaaga acttgcaaaa ccagctatag atattcttaa aaaaggagaa ttagaatttg
    14041 ttccagataa atttgataaa acttatttac aatggctaga aaatataaga gattggtgta
    14101 tatcaagaca attatggtgg ggtcatcaaa taccagctta ttattgtcaa gagtgtggag
    14161 aaatagtagt tgctagaaaa atgccagata agtgtcctaa atgtggaagc actcatttta
    14221 aacaagatga agatgcctta gatacttggt tcagttctgc attatggcct ttctctactc
    14281 taggatggcc aaataagact gaagctttag attattatta tccaacaagt gtacttgtta
    14341 caggatacga tataatattc ttctgggtag ttagaatggc atttgcaggt atgttctgta
    14401 tgaatgaaaa gccatttgac catgttttgg ttcatggatt agtaagagat tctcaaggta
    14461 gaaaaatgag taaatcttta ggaaatggta tcgacccatt ggagataatc gagcaatatg
    14521 gagctgatgc cctaagattt acattaacaa ctggaaattc cccaggaaat gatatgagat
    14581 tctatatgga aagagtagaa tttgctagaa actttgcaaa taaattgtgg aatgcatcaa
    14641 gatttgtatt tatgaatatt gatgaagata taattaaaaa catgactaga gaaagtgtta
    14701 aagaggactt aactctagct gataaatgga taatatcaag agcaaataat attgtaaaag
    14761 aagcaactaa taacatggat aagtttgatt taggaatagc acttcaaaag atatacgatt
    14821 ttacttggtc agaatactgt gactggtata ttgaaatggt taaacctaga ttatatgggg
    14881 aagatgcaaa tgctaaatct gcagcattat atacattaac ttatgttctt gaaaaaatat
    14941 taaaattatt acatccatac atgccattta taacagagga aatatatact catcttccta
    15001 ctgttgaagg atgtataata gtatctgaat ggccaaaata taatgaagaa gataacatgg
    15061 ctgaagaaga agatatgatg aacttattga tggaaggtat aagaagtata agaaatgtga
    15121 gagcagaaat gaatgtacca ccttctaaga aagctaaatt gataataatc ccaagtgaag
    15181 aaaaaataga agctattgag cttggtaaag actattttat aactttagca tctgcttcaa
    15241 atgtagagat agcaaaagat aaatctaatg ttccagaaga tgcagttgga gtagttattg
    15301 atggtgttga aatatttata cctcttaatg aattagttga ttttgaaaaa gaaattgaga
    15361 gattatctaa agaaaagaaa aagttagaag gcgaaataaa gagagttaat ggaaagcttg
    15421 caaatcaagg tttcttagca aaagctcctg aaagtttaat tgaagaagaa aaagcaaaaa
    15481 aagaaaaatt tgaagagatg attaaatctg ttgaggaaag attaacaaat ttagaatcta
    15541 aaataaaata aatataagaa ttagtaaaaa catacaaaaa gtatatattc tatacttttt
    15601 gtatgttttt ctttatata
//
LOCUS       DAELLV010000075        15578 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.075, whole genome shotgun sequence.
ACCESSION   DAELLV010000075 DAELLV010000000
VERSION     DAELLV010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15578
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.075"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            204..1280
                     /locus_tag="KPD58_001853"
     CDS             204..1280
                     /locus_tag="KPD58_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG0265623.1"
                     /translation="MKAQRLNAVLEQMKKDDISQMLVSDPTSIFYLTGVLIHPGERLL
                     ALYLNLNGNNKLFINELFPVSEDLGVEMVWFNDTQNPVEIITEHIDKNATMGVDKNWP
                     ARFLLNLIELGGGSKFVNSSYIIDTLRMCKDEEEKELMRIASKLNDKAMEQLKATVSG
                     ELTEKQLVGKLSKIYEDLGTDGFSFDPIIGFGPNGANPHGEPGNALVKPGDAIILDIG
                     CIKDNYCADMTRTVFYKEIPEKGREIFEIVLEANKRAEAIVKPGVRFCDIDAAARDYI
                     TEKGYGQYFTHRTGHSIGLEVHDKGDVSSINTDTVQPGMIFSIEPGIYLPGEFGVRIE
                     DLVLVTEDGCEILNKHDKEICVVG"
     gene            complement(1397..2239)
                     /locus_tag="KPD58_001854"
     CDS             complement(1397..2239)
                     /locus_tag="KPD58_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBG0265624.1"
                     /translation="MDKIKLIVDSACDLPDDIIEKYNIEVVGLNVSFGEESYISGKEI
                     DNETFYRKMSESKVLPKTSCPSPDKFLEAYHCQEESVLVINISSGLSGTYNSALLAKD
                     MFEKEGNNKKVEVIDSLSGSIGIGQLVLKAAILIKEGKEIEEIVSIINKYKDNPPFFG
                     TLETLENAIKGGRINPIAGKIINTLNFKAIIQVADGVVTPIDKARGEGNSLKKLITLV
                     ESKIKDKEEKVLFIGHANCPEKAQKVREVMEKNVKYKDVVICEIGSVMGTYTSKGAIL
                     ITAI"
     gene            2423..2995
                     /locus_tag="KPD58_001855"
     CDS             2423..2995
                     /locus_tag="KPD58_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0265625.1"
                     /translation="MKTNNMKQAILSSATNLIAKNGVQNTSLADIAKDVNISKGTLYY
                     HYASKDDIIYDIADTHLEVITSAILNCVKNVKSKNSQIEMVNLILEKISTIESRGRVH
                     MYLICEAITSNNDLKERIRLKYIEWRTTLKSEIVESLEKYNSSENEQDAESFSFLLMS
                     IVDGLVVQSLLKTEKIPYENIASFLVNHWI"
     gene            complement(3158..3430)
                     /locus_tag="KPD58_001856"
     CDS             complement(3158..3430)
                     /locus_tag="KPD58_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265626.1"
                     /translation="MLRERFVVRNHHNPHTSGCCGTGGEGCCGGDNHEHSHQHSHSHG
                     HEHEHVNGVGHDHNHDDGFSGTHEHSHEHTHTHGHEHSDVHTHEHK"
     gene            complement(3557..4429)
                     /locus_tag="KPD58_001857"
     CDS             complement(3557..4429)
                     /locus_tag="KPD58_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG0265627.1"
                     /translation="MYGESKKLSIDNNIFLQYCLIILGMILSTLGINLYLAPAKLLSG
                     GVAGICVILYKLFGINQGISSFLMNIPIFIIARKYFDNKFLLISFVNMLLFSLALGLT
                     QDIAKYFPIDDTMLQCIYGGALTGIGMGLTFKARATAGGLDIIAAIMKRKYDIPMKNT
                     FLFINFFVVCAGAFLFGAKLVMYTLITMYIISFTMDIGKDCFDRKKSILLISNKYDEI
                     SKVIMNKMGRGVTFLEAEGAYTQNKKKMIYCIISANEIGKFKDLIYDMDSEAFISVNN
                     VEEVKGGGFKDKFL"
     gene            complement(4725..7832)
                     /locus_tag="KPD58_001858"
     CDS             complement(4725..7832)
                     /locus_tag="KPD58_001858"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBG0265628.1"
                     /translation="MAKFKPLVDSSVKQAEAQVFDYWKDINILEKTLEKGKDDPSFVF
                     YEGPPTANGNPGVHHVLSRTLKDSVCRYKTMSGYQVKRKAGWDTHGLPVEIQVEKELG
                     LTSKQQIEEYGIAEFNQKCRESVFSFEKQWRIMTERMAYEVDLDNPYITLDNNYIESV
                     WWILNKFNKEGYIYEGHKILPYCPRCGTGLASHEVAQGYKEVKTNTVIAKFKKKDADE
                     YFLAWTTTPWTLPSNVSLTVNADVDYLKVKKGDEVYYVSKPLADKVLGEDYEVLEEMK
                     GKDLEGLEYEQLMPFVEVDKKAFFITVGDYVTTEDGTGIVHTAPAFGEDDYNTGKRYD
                     LPVIQPVDETGKFTSTPWEGRFVMEDGLDVEIIKWLASENKLYSKEKVVHNYPHCWRC
                     QTPLVYYAKPSWYIEMTKLKDKLIENNNGVKWFPSFVGEKRFGNWLENLNDWAISRTR
                     YWGTPLPIWRCECGHTDSVGSRAELAEKAIEDVNPETVELHRPYVDDIHLKCEKCGKP
                     MTRVTEVIDCWFDSGAMPFAQHHYPFENKENFDQLFPADYISEGIDQTRGWFYSLLAV
                     STFVLGKAPYKSVLVPDLVLDKDGKKMSKSRGNTVNPMELFDQYGADALRWYLLYVSP
                     PWTPTKFDMDGLKEIQSKFIGTMKNVYNFFTLYANTDDINPTEFFVEYKDRPELDRWI
                     LSKFNNLMKDVEENLAIFELNKTLRMIQDFINDDLSNWYIRRSRRRFWATELTEDKKA
                     VYNTTYELLHGLCRAIAPFAPYMSEEMYRNLTGEVSVHLAEYPKCNEELVDTKLEEKM
                     DLAKNLVTLGRASREVERIKVRQPLQKVLVDGKFEDTISDVVDLIKEELNVKEVIFAK
                     DLDEYMNFSLKPNFKEAGPLLGSKMNLFVGALSKLNAHETANKLEKGETLTVDLDGEA
                     FEFNKDLVLIGITAKEGFNVSVENNLFVILDTKLTEELINEGYAREFISKVQQLRKSN
                     NFEVLDNIVIEYCGDDEIAKAVDHFNEYIKSETLALEINRVDDKSLEEQNLNDHMTGI
                     KVIKK"
     misc_binding    complement(7925..8157)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8332..8859)
                     /locus_tag="KPD58_001859"
     CDS             complement(8332..8859)
                     /locus_tag="KPD58_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HBG0265629.1"
                     /translation="MLTPIEIENKEFKKGLRGYRDEEVDEFLDIIKEDYESLCRENTA
                     LKEKLGLYQDQVNKYENIEETLKATLITAQSAAEDTCSAANKKAKIIVEEADLKARQI
                     IEQANNRVIEIRKEYDALVKEFKIFRNKFKSLLEDEIRSVDEIFYDVDEKCVNAFEGT
                     TVYNYSNDEAATTLE"
     gene            complement(8894..9679)
                     /locus_tag="KPD58_001860"
     CDS             complement(8894..9679)
                     /locus_tag="KPD58_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBG0265630.1"
                     /translation="MDKLKLTNHIKDIDLKNKMFKVIDKANSCIKNYDVKSTEFLNPY
                     EVKNAVAILNSTNEIKYSVDGGYEQAERSTVFIYPFYMEYEDIEDTLRFLQIEGNFKF
                     KNISHKDYLGSILGLGIKREKIGDIIIHDSFCQVVVSSDICDFIIVNLEKVSRNNVIV
                     KEISRENIVNSSSKYKEVSFTVSSDRLDCVISGIYNISRQDSAKYINGEKVHVNYEKI
                     TSTSKIVKNDDLISIRGKGRAKVTQIGDITKKGKIKVQARLIV"
     gene            complement(9689..9946)
                     /locus_tag="KPD58_001861"
     CDS             complement(9689..9946)
                     /locus_tag="KPD58_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HBG0265631.1"
                     /translation="MGTIRIALYYLLDIIAWMIVIKSLMTWFPNGRQSKIFDILENLT
                     EPIEGPIRSIMYKYTNGPIDFSPLIAIVLLMFLRQLVLVIF"
     gene            complement(9965..10423)
                     /locus_tag="KPD58_001862"
     CDS             complement(9965..10423)
                     /locus_tag="KPD58_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HBG0265632.1"
                     /translation="MSNGIISKFKNWIVDEEEDYIEDEYESGMDDIVQEEEMNSGFST
                     AKANKIVNLHTTSQMKVVIVEPKVYDEAATIADHLKQRRAVIVNLEGLTNSEVRKSIF
                     NFMNGAVYVLDGSIQKVSKSIFILAPNNVDIDANMKKELESKAFFPWQNK"
     gene            complement(10463..11167)
                     /locus_tag="KPD58_001863"
     CDS             complement(10463..11167)
                     /locus_tag="KPD58_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HBG0265633.1"
                     /translation="MGSIKDNLSEIRNKIDKAAKKVDRDSKEITLLAVTKTVDVDLVN
                     EAMREGITSVGENKPQELARKYDIIGDKLNWHLIGTLQTNKVKYIIDKVCMIHSLDRI
                     ALCEEIQKRAEQINREIDCLVQVNISKEESKHGLDREEVVDFVKEVSEKYKNIKIKGL
                     MTMAPFIEDEDEIRGVFKGLKDLSKQIKELNIPNVGMDVLSMGMSHDYEIAIEEGATI
                     VRVGTSIFGERNYNKK"
     gene            complement(11177..11812)
                     /locus_tag="KPD58_001864"
     CDS             complement(11177..11812)
                     /locus_tag="KPD58_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265634.1"
                     /translation="MKKVKYTRLVILGILIYLVFNILVMIISKNVDTLVLKNEKVDAK
                     IHVKGSIEKDANDQSNYLSTYISKEDSNNFEKNQNVIIEYHEQKLDAKVYKIYENNNK
                     IMVKLKISNQIVANQDTSVEEFDIIYNQMECLKIPKTSIKTKDNKRGVYVIDEQSQSV
                     KFVILEGITYENESSVFVDYYKNDINGVTSVNLYDKIILRPNSINTNIRIK"
     gene            12193..13233
                     /locus_tag="KPD58_001865"
     CDS             12193..13233
                     /locus_tag="KPD58_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0265635.1"
                     /translation="MSEILLGTGLLLLVLCCFTIFSYKAPYGMKAMGALANAACATFL
                     VEAFNLSLLGDVLGMEFFRSVGAANGSLGGVAAAILVPLALGVSPVYAVLIGLSCLDF
                     SILSGFVAGYFVSFLIKFIEKKVPPGVDLVVVILLCAPLSRLIASNVDPLVNSTLLQI
                     GQVLIEATNISPIIMGIILGGLITVVGTSPLSSMALTSIIGLTGLPMAIGALGAFGSA
                     FMNSTLFAKLKLGSKKDIISVAIEPLTQSDIISANPIPIYSTNFFGGAFSGIIVALMG
                     LVNMTPGTATPVAGFAIMFAYNPVQQVLITAGLCALFNVIAGFLGSKIFSTFKVLTAG
                     EIRGTDVEEEIA"
     gene            complement(13696..15051)
                     /locus_tag="KPD58_001866"
     CDS             complement(13696..15051)
                     /locus_tag="KPD58_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HBG0265636.1"
                     /translation="MNVGNILETIKMIDEEQLDIRTITMGISLLDCIDPDGDKAREKI
                     YNKIMSSAKDLVKVGKDIEREFGIPIVNKRVSVTPISIIAGATDENDYVKFAQTLDKA
                     AEDLGIDFIGGFSALVQKGYTKGDKILIKSIPKALASTNKVCASVNVGSTRCGINMDA
                     VKEMGEIIKETAELTKDAKGFGCAKLVVFCNAVEDNPFMAGAFHGVGEADRIINVGVS
                     GPGVVKRALEKVKGEPFDVVSETVKKTAFKITRVGQLVAKEASSRLDVPFGIIDLSLA
                     PTPAIGDSVANILEEMGLEVVGTHGTTAALALLNDAVKKGGVMACSHVGGLSGAFIPV
                     SEDAGMIDAVIKGALSIDKLEAMTAICSVGLDMIAVPGNTTAGTLGAMIADEAAIGMI
                     NNKTTAVRIIPAPGCDVGDMVEFGGLLGRAPVMPINTNSSELFTQRGGRIPAPIHSFK
                     N"
     gene            complement(15077..15346)
                     /locus_tag="KPD58_001867"
     CDS             complement(15077..15346)
                     /locus_tag="KPD58_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBG0265637.1"
                     /translation="MKAILTVIGKDKPGIVAGISDELYKQNINIVDISQKILQDYFTM
                     IMIVDLEKSLNSFEKTVEDLVERGKKLSVDVKIQREEIFNSMHNL"
BASE COUNT         4951 a         2703 c         1801 g         6123 t
ORIGIN      
        1 tatagactta atttattact tttatagaat tttcacaata aaaagtcatt ttacttccgt
       61 atttaatgca aaagtagaga ttaaacttaa tataaaaaat atataacaaa ttttttttat
      121 atttctagaa gaaaagtata ctcttttttg tttaataatg ctaaaattga tttgtattga
      181 attttgatta ggaggaaatt attatgaaag ctcaaagatt aaatgctgta cttgaacaaa
      241 tgaaaaaaga tgatatttct caaatgttag taagtgaccc tacttctata ttttatctta
      301 caggagtact aatccatcca ggcgaaagac tattagcatt atacttaaat ttaaatggca
      361 ataataaatt atttataaat gaattattcc cagtttcaga agatttaggt gttgaaatgg
      421 tttggtttaa tgatactcaa aatccagtag aaataataac tgaacatata gacaaaaatg
      481 ctactatggg tgttgataaa aactggccag ctagattctt attaaactta atagaattag
      541 gtggaggaag taagtttgta aattcatctt atataataga tactttaaga atgtgtaaag
      601 atgaagaaga aaaagagtta atgagaatag cttcaaaatt aaatgacaag gctatggaac
      661 aacttaaagc tactgtttct ggtgaactta cagaaaaaca acttgttggt aaattatcta
      721 aaatatatga agacctaggt actgatggat tttcatttga ccctattata ggttttggac
      781 ctaatggagc taatccacat ggtgaacctg gaaatgcact tgtgaaacca ggagatgcaa
      841 taatcttaga tataggttgt ataaaagata actattgtgc tgatatgaca agaactgtat
      901 tctataaaga aatacctgaa aaaggtagag aaatatttga aatagttctt gaagctaata
      961 agagagctga agctattgta aaaccaggtg ttagattctg tgatatagat gctgctgctc
     1021 gtgactacat aactgaaaag ggatatggac agtacttcac tcatagaact ggtcactcta
     1081 taggacttga agttcatgat aaaggagatg tatcttctat aaatacagat actgtacaac
     1141 caggaatgat attctctata gagccaggta tatatttacc aggtgagttc ggagttagaa
     1201 tagaagattt agtacttgtt actgaagatg gttgtgagat attaaacaag catgacaaag
     1261 aaatatgtgt agttggttaa ttctaaaatc aatttgataa aattaaaaag ccatataaaa
     1321 atgttttaaa catttttata tggctttaat ttctcaatct attatttatt tcaatccatt
     1381 atttatattg cagtatttat attgcagtta ttaagattgc accctttgaa gtatatgtcc
     1441 ccatcactga accaatttca cagattacaa catctttgta ttttacattc ttttccataa
     1501 cttcccttac cttttgagcc ttttctgggc aattagcatg tcctataaat aaaacctttt
     1561 cttctttatc tttaattttt gactctacta aagtaatcag ctttttcaaa ctgtttcctt
     1621 cacctcttgc cttatctata ggagttacaa cgccatctgc aacttgtatt atcgccttaa
     1681 aatttaaggt atttataatt tttcctgcta ttggatttat tctaccaccc ttaatagcat
     1741 tttctaaagt ttcaagtgtt ccaaaaaatg gaggattatc tttatattta tttattattg
     1801 aaacaatctc ttcaatttct tttccttctt ttattaatat tgctgctttt aacactaatt
     1861 gtcctattcc aattgaccca ctaagtgaat ctataacctc aactttcttg ttatttcctt
     1921 ccttttcaaa catatctttt gctaaaagtg cactattata agtcccagat aatcctgaac
     1981 ttatattaat cactaataca ctttcttcct gacaatggta tgcttctaaa aatttatcag
     2041 gagaaggaca tgatgttttt ggtagtactt tactctcact catttttcta tagaaagttt
     2101 cattatctat ttcctttcca gatatataac tttcttctcc aaaagataca tttaaaccaa
     2161 caacttctat gttgtatttt tctattatat catcaggtaa gtcacatgca ctgtccacta
     2221 tcaattttat tttatccata ttaacctcac tataagatat tattttattt attcagtcgg
     2281 tcgtacgacc gatttaatta ttattttata tattgaatga aaaaaagtca atagatatag
     2341 attctcgctt tattgacaat aaatatccat aagcttataa tgttattaat ttgttataaa
     2401 tatacatgga aaggtaggtt ttatgaaaac aaataatatg aaacaggcta ttttatcatc
     2461 tgcaaccaat ttaatagcca aaaatggagt tcaaaatact agccttgctg atattgcaaa
     2521 agatgtaaat ataagtaaag gtactttgta ttatcactac gcatctaaag atgatattat
     2581 atatgatatt gcggatactc atttagaagt tataactagt gctatactaa actgtgtaaa
     2641 aaatgtcaaa tcaaaaaatt ctcaaataga aatggttaac ttaatcttag agaaaatatc
     2701 tactatagaa agtcgtggta gggtacatat gtatcttatt tgtgaggcaa ttacttcaaa
     2761 taatgattta aaggaaagaa taagattaaa atatattgaa tggagaacta ctcttaaatc
     2821 tgaaattgtt gaatcactag aaaaatataa tagtagcgaa aatgaacagg atgctgaatc
     2881 attttctttt ttactcatgt caatagttga tggtttagtt gtccaaagtt tattaaaaac
     2941 agaaaaaata ccttatgaaa atatagcttc ttttttagtt aatcattgga tttaaactta
     3001 atacttagat aattaataaa agtgaactat ttaagttatc tactttaaca gttcactttc
     3061 ttatttattc tcaataattt ttacttaaaa cattcaattt ttatttaaac cttcatggaa
     3121 tcacaaaata aaaatctctt ataaatttaa aatgttttta tttatgttca tgtgtatgca
     3181 catctgaatg ttcatgacca tgtgtatgtg tatgctcatg gctatgctca tgagtacctg
     3241 aaaaaccatc atcatgatta tggtcgtgac ctacaccatt tacatgctca tgttcatgcc
     3301 catgactgtg agaatgttga tgtgaatgtt catggttatc tcctccacag catccttctc
     3361 ctccagtccc acaacaccca cttgtatgtg gattatgatg atttcttact acaaatctct
     3421 ctcttaacat aattcttata cctcctagta tattattgag tacttcatgt accccattaa
     3481 ctacagtata catctaacta ggtataatct caatacatat ggcatttttt aacatatata
     3541 attaacatat ataatcttaa agaaatttat ccttaaaacc tccacctttt acttcttcaa
     3601 cgttattgac agaaataaaa gcttcactat ccatatcata aattaaatct ttaaatttac
     3661 ctatttcatt tgcagatatt atgcaataaa tcatcttttt cttattttgt gtatatgcac
     3721 cttctgcctc caaaaaagtg actcctctac ccattttatt cattatcact ttggatattt
     3781 catcatattt atttgatatt aaaagtatag atttttttct atcaaagcaa tcttttccta
     3841 tatccatagt aaaagaaatt atatacattg taattaaagt gtacatcacc aatttagctc
     3901 caaataaaaa agctcctgca catactacaa aaaaattaat aaataaaaat gtatttttca
     3961 taggtatatc atactttctt ttcattatgg ctgctattat atcaagtcct ccagcagtag
     4021 ctcttgcttt aaaagttaac cccataccta taccagttaa tgcaccacca taaatacact
     4081 gtagcatagt atcatctatt gggaaatatt tagcaatatc ttgtgttagc cccagtgcta
     4141 gagaaaatag taacatattg acaaaactta ttagcaaaaa tttattatca aaatactttc
     4201 tagctataat aaatattggt atgttcatca agaaacttga tattccctga ttaataccaa
     4261 ataatttgta taatataaca caaattccag ctactcctcc acttaataac ttggctggtg
     4321 ctagatataa atttattcct aaggttgata gaatcatacc caatataata agacaatatt
     4381 gtaaaaatat attattatca atacttagct ttttactttc accatacatc aaaacgcacc
     4441 cctttgcata ataaataaac caccctgcat aacacacgat aaatttacaa ataaatttat
     4501 cagaagtagt ttcctaaaaa tattattcat cctcacttaa acgtttattc attaatattc
     4561 tatcacatga cataaataat ttatatgatt tatatagatt tatttattgt aaattttgga
     4621 atattaaaac attttattga atagataatt atattacata aaaaatccct atagaaatct
     4681 ataaactaat ttctataggg attttggtta actaactatt atttttattt ttttataact
     4741 tttatacctg tcatatggtc atttaagttt tgttcctcta aagatttatc atctactctg
     4801 tttatttcta aagctaaagt ttcagacttg atatattcat taaaatggtc aacagcctta
     4861 gcaatttcat catcaccaca gtattctatt actatattat ctaatacttc aaagttatta
     4921 gattttctta attgctgtac tttagatata aattctctag cataaccttc attaatcaac
     4981 tcttcagtta gcttagtatc tagtataaca aaaagattgt tttcaacaga tacattaaat
     5041 ccttccttag cagttatacc tattaatact aaatccttat taaattcaaa agcttcacca
     5101 tctaaatcaa ctgttaaagt ctcacctttt tcaagtttat tagcagtttc atgagcattt
     5161 aatttactta atgctccaac gaataaattc atcttagaac ctaataatgg ccctgcttcc
     5221 ttaaagtttg gttttaaact aaagttcata tactcatcta aatccttagc aaatattacc
     5281 tctttaacat taagctcttc ttttattaaa tcaactacat cacttattgt atcttcaaac
     5341 ttaccatcta ctaacacttt ttgtaatggt tgacgaactt ttattctttc tacttctctt
     5401 gaagctctac ctaaagtaac taagttttta gctaaatcca ttttttcttc taatttagta
     5461 tctactaact cttcattaca tttaggatat tcagctaaat gaacagatac ttctcctgtt
     5521 aagtttctat acatttcttc tgacatatat ggtgcaaatg gagctatagc tctacaaaga
     5581 ccatgtaaca attcataagt tgtattgtac actgcttttt tatcttctgt taattcagtt
     5641 gcccagaatc ttcttcttga acgtcttata taccagttag ataaatcatc atttataaaa
     5701 tcttgtatca ttcttaaagt tttatttaac tcaaatatag ctaaattttc ttctacatct
     5761 ttcattaaat tattgaactt agataatatc catctatcaa gttctggtct atctttatat
     5821 tctacaaaaa attcagttgg atttatatca tctgtgtttg catataaagt aaagaagtta
     5881 tatacattct tcatagtacc tataaattta ctttggattt cttttaatcc atccatatca
     5941 aatttagttg gagtccatgg tggagaaaca tatagtaaat accatcttaa tgcatctgct
     6001 ccatattggt caaataattc cattggattt actgtatttc ctcttgattt actcattttc
     6061 ttaccatctt tatcaagtac aaggtctgga actaaaacac tcttatatgg tgcttttcct
     6121 aatacaaatg ttgatactgc aagtaatgag tagaaccaac ctctagtctg gtctatacct
     6181 tctgatatat agtcagctgg gaacaattgg tcaaagtttt ccttattttc aaatggatag
     6241 tggtgttgag caaatggcat agccccacta tcaaaccaac agtctataac ctctgttact
     6301 cttgtcattg gttttccaca tttttcacat tttaagtgta tatcatctac atatggtcta
     6361 tgaagttcaa ctgtttctgg atttacatct tctatagctt tttcagctaa ttcagctctt
     6421 gaaccaactg aatctgtatg accacattca catctccata taggaagtgg agttccccaa
     6481 tatctagttc ttgatattgc ccagtcattt agattttcta accagtttcc aaatctcttc
     6541 tctcctacaa aacttgggaa ccattttaca ccattattat tttctattaa tttatcttta
     6601 agtttagtca tctctatata ccagcttggt tttgcatagt atactaatgg agtttgacat
     6661 ctccagcagt gtggataatt atgaactact ttttctttgc tatataattt attttcagaa
     6721 gctagccatt ttattatttc aacatctagc ccatcttcca ttacaaatct accttcccaa
     6781 ggagtagatg taaatttacc agtttcatct actggttgaa taactggtaa atcatatctt
     6841 ttacctgtgt tgtagtcatc ttcaccaaat gctggtgctg tatgaactat accagttcca
     6901 tcttcagtag ttacataatc tcctactgta ataaaaaatg cttttttgtc aacttctaca
     6961 aaaggcatta attgttcata ttctaaacct tctaggtctt tacctttcat ttcttctaaa
     7021 acttcataat cttcacctag tactttatca gctaaaggtt tagatacata atatacctca
     7081 tcaccttttt taacttttaa ataatctaca tcagcattta ctgttaatga aacatttgat
     7141 ggtaaagtcc atggagttgt tgtccatgct aagaagtatt catcagcatc ttttttctta
     7201 aatttagcta taactgtatt agtttttact tctttatatc cttgtgcaac ctcatgacta
     7261 gcaagaccag ttccacatct tgggcaataa ggaagtattt tatgcccttc atatatatat
     7321 ccttctttgt tgaatttgtt tagtatccac catacagact ctatatagtt attatctaaa
     7381 gttatatatg gattatctaa atcaacttca taagccattc tctcagtcat tattctccat
     7441 tgtttttcaa atgagaaaac tgactctcta catttttggt taaattcagc tattccatat
     7501 tcttctattt gttgcttact agtaagtcct aactctttct ctacttgtat ttcaactggt
     7561 aatccatgtg tatcccaacc agcttttctc tttacttgat aaccagacat tgttttatat
     7621 ctacaaactg aatctttaag cgttctagat aaaacatggt gtactccagg atttccatta
     7681 gctgttggag gtccttcata gaatacgaaa cttgggtcat ctttcccttt ttctaaagtt
     7741 ttttcaagta tatttatgtc tttccaataa tcaaaaactt gtgcttctgc ttgttttaca
     7801 gaactgtcta ccaatggctt aaatttcgcc atgtaaatca ccctttccta ctaaatatta
     7861 taattccaaa attttttatt ctttcacata aatttaaata ctaaaaaact cgcccccttt
     7921 aaaccaaagg gacgagttat aaatcgcgtt accaccctaa ttctatatgt aaactaaata
     7981 ctagctacat ataacactct aatagtataa cggtactaac cggcacagct tagtagaatt
     8041 taatcgttca gcctgcagct caagagtgat ttttatcttt ataaattcat tggttctcag
     8101 catacccaat tctctgtaaa attatctaaa gataaatgtc tctatcaacg cttttaaaaa
     8161 tttatctgtg tttatatgaa attctataac aaataatttg aatagtcaat atatttttga
     8221 aaaatataat aatttctatt tttcttacat ttttatccta tttttaattt ttgttataga
     8281 tttctcaaac tcacacaatt attctacata aaaaataaac aaattacaag cttattctaa
     8341 agttgtagca gcttcatcat tactataatt atacactgtt gttccttcaa atgcgtttac
     8401 acatttttca tctacatcat aaaaaatctc atcaacactt cttatttcat cttctaacag
     8461 tgatttaaat ttatttctaa atatcttaaa ttctttgact aaagcatcat actcttttct
     8521 tatctctata acacggttat tagcttgttc tataatttgt ctagctttta aatcagcttc
     8581 ttcaacaatt atcttagcct ttttattagc cgcgctacaa gtgtcttctg ctgcactttg
     8641 agcagttatt aaagtagctt ttagtgtttc ttcaatattt tcatacttgt ttacttggtc
     8701 ttgatataac cctaattttt ctttaagagc tgtattttcc ctacatagag attcataatc
     8761 ttccttgatg atatctaaaa attcatcaac ctcttcatct ctataccctc ttaaaccttt
     8821 tttaaactct ttattttcta tctcaattgg agttagcatt acatttcctc ccagctataa
     8881 tttaaattag tccttataca attaatcttg cttgaacttt tatttttcct tttttagtaa
     8941 tatctcctat ttgtgttacc ttagctcttc ctttgcctct aatagaaatc aaatcatcgt
     9001 tttttactat tttagaagtt gaagttatct tttcataatt gacatggact ttttctccat
     9061 ttatgtattt tgcactatct tgtctagata tattgtaaat accacttatc acacaatcta
     9121 acctatctga agatactgta aatgatactt ctttatattt tgaagaacta ttaacaatat
     9181 tttctctgga aatttccttt acaattacat tatttcttga aaccttttct aagtttacaa
     9241 ttataaaatc gcaaatatca ctacttacaa ctacttgaca aaagctatca tgtataatta
     9301 tatctccaat tttttctctt tttataccta gacccagtat tgaccctaag taatctttat
     9361 gagatatatt tttaaatttg aaattccctt ctatttgtaa aaatctaaga gtatcttcta
     9421 tatcttcata ttccatgtaa aatggataaa taaatacagt gcttctttca gcttgttcat
     9481 aacctccatc cacactgtat tttatttcat ttgtagaatt taatattgct acagcatttt
     9541 tcacttcata tggattaaga aactccgtag acttaacatc atagtttttt atacaactat
     9601 tagccttgtc tatcacttta aacattttat ttttcaagtc tatatccttt atgtgatttg
     9661 ttagttttaa tttatccatc ctatcacctt agaatataac caatactaat tgtcttagga
     9721 acatcaataa tactattgca attaaaggag aaaaatctat aggtccattt gtatatttat
     9781 acattataga ccttattggc ccttctattg gctctgtaag attttccaaa atatcaaaaa
     9841 ttttactttg tcttccattt ggaaaccaag tcatcaagct ttttataact atcatccatg
     9901 ctattatatc taaaagataa taaagtgcaa ttcttattgt tcccatatat taaccgcctt
     9961 tttactattt attttgccaa gggaagaatg ctttactttc taattctttt ttcatatttg
    10021 catctatatc cacattatta ggagctagta taaatattga tttagaaacc ttttgtatac
    10081 ttccatctaa gacataaact gccccattca taaagttaaa tatagatttg cgaacttcag
    10141 aattagtcaa tccttccaaa ttcactataa ctgcccttct ttgttttaga tggtctgcta
    10201 tagttgcagc ttcatcataa accttaggtt caactattac aactttcatt tggctagttg
    10261 tatgtaaatt tacaatttta tttgcttttg cagtactaaa gccactgttc atttcttctt
    10321 cttgaactat atcgtccatg ccactttcat attcatcttc aatgtaatct tcttcttcat
    10381 caactatcca attcttaaat ttagatatta ttccatttga cattattaac ttcctcctta
    10441 aaatttattc aaatttgagt aattattttt tattgtagtt tctttctcca aagattgatg
    10501 ttccaactct aacaatggta gccccttcct ctatagcaat ttcataatcg tgactcattc
    10561 ccattgacaa aacatccatc cctacattag ggatgtttag ttcttttatt tgttttgata
    10621 aatctttaag acctttaaat acacctctta tctcatcttc atcttctata aaaggtgcca
    10681 ttgtcattag accctttatt tttatatttt tatatttttc tgacacttct ttgacaaagt
    10741 ctacaacttc ttctctatca agtccatgct tagactcttc tttagatata ttcacttgta
    10801 ctaagcaatc tatttctctg tttatttgtt ctgcacgttt ttgaatttct tcgcataatg
    10861 caattctatc taaagagtgt atcatacaaa ctttatcgat tatgtactta actttattgg
    10921 tttgcaaagt tcctatcagg tgccagttta atttatcacc tataatatca tactttctag
    10981 ctaattcttg gggtttattt tcaccaacag aagtgattcc ttccctcatt gcctcattaa
    11041 ctaagtctac atcaacagtc tttgtgactg ccaaaagggt aatctctttg ctgtctctat
    11101 ctactttttt tgcagcttta tcaattttgt ttcttatctc agacaaatta tctttaatag
    11161 aacccattat tatcacctac tttatcctta tatttgtgtt tatactgtta ggtcttaaaa
    11221 ttattttgtc atatagatta acacttgtaa ctccatttat gtcattttta taataatcca
    11281 caaatacaga agattcattc tcataagtta ttccttctaa tatgacaaat tttacacttt
    11341 gtgattgttc atcaattaca tatacaccac gtttattgtc ttttgttttt atagaagttt
    11401 taggtatttt taaacattcc atctgattat atattatatc aaattcttcg acacttgtat
    11461 cctgatttgc aacaatttgg ttacttattt ttaattttac cattatttta ttattatttt
    11521 catatatttt gtaaactttt gcatctaatt tttgctcatg atattcaatt attacatttt
    11581 gatttttttc aaagttatta gaatcttctt tactgatgta tgtagataga taattactct
    11641 ggtcattggc atctttttct atacttcctt ttacatgaat ctttgcatca actttttcat
    11701 tttttaacac caacgtatct acatttttgc taattatcat tactaatata ttaaatacta
    11761 aatatataag aataccaagt atcactaatc tggtatattt tacctttttc aaagactctc
    11821 cccctataaa attactttca ttataagtac ttcttctaaa aaatgtttat tcctttttta
    11881 taagctaata ttttacgttc taataattat tttttccata ttaaatttat tttttatcaa
    11941 tcttcctata caagactgca ttatttatat ataattgtat tataaagtat aagtttaatt
    12001 tacattttat acgttaagct tactttatat gtttttatag ttatttttat ttttagatgc
    12061 aatataaaac caattaagat ttaaaataac ttttgttatg ttgacattta tttaggtaat
    12121 catttatgaa ttaggataac gttatcataa caaaagttta tgtcctaaaa aaaatacaag
    12181 gaggattttc ttatgtcaga aattttatta ggaacaggtt tacttttatt ggttctatgt
    12241 tgtttcacaa tttttagtta caaggcccca tatggtatga aggctatggg agctctagca
    12301 aacgctgcat gtgctacttt cttagtagag gcattcaacc tttctttact tggtgatgtt
    12361 ttaggtatgg aattttttag aagtgttgga gctgctaatg gtagtcttgg tggagttgct
    12421 gctgctatac ttgtgccttt agccttaggt gtatctccag tttatgcagt tttaatagga
    12481 ttatcttgtt tagatttttc tatattatct ggattcgttg ctggatactt tgtttctttc
    12541 ttaattaagt ttatagagaa aaaagtgcca cctggagtgg atttagtggt tgtaattctt
    12601 ctatgtgcac cgctatctcg tcttatagca tcaaatgtag acccattagt taatagtaca
    12661 ctactacaaa ttggacaagt tttgatagaa gctaccaaca ttagccctat aataatggga
    12721 attatactag gaggtctaat aacagtagtt ggaacgtctc cacttagttc aatggctctt
    12781 acatctataa ttggtcttac tggtcttcct atggcaatag gagcattagg tgcttttggt
    12841 tcagctttta tgaactcaac actatttgca aaattgaaat taggttctaa aaaagatatt
    12901 atatctgttg ctattgaacc attaacccaa tcagacataa tttcagctaa tccaatacct
    12961 atatattcta ctaatttctt tggaggagcc ttttctggta tcattgtagc cttgatgggt
    13021 cttgtaaaca tgacaccagg tacagctaca cctgtggctg gttttgctat aatgtttgct
    13081 tataatccag ttcaacaagt tcttataaca gctggtttat gtgctttatt caatgtaatt
    13141 gcaggatttt taggttcaaa aatattcagt acctttaaag ttcttactgc tggtgaaata
    13201 cgtggtactg atgtagaaga agaaattgct taaaaaacaa taagatttat aaatttatat
    13261 ctcaagtagt agagttttat attgtaggca attttattaa ttgcaaacgt tttatttttt
    13321 atttatgttt atgattatta tgtgtgttga atatttagat aaatttttat ttattatttt
    13381 tatttagatt tttgtttagt tttttatttg gttgatatat taggaataaa aattagtata
    13441 gtttattact agttaagtag cataactttt tagatacata aaaaatttta tattaattag
    13501 atattattaa aataaaagcg atacatgaat ttttaaatgt ccatgtatcg ctttattttt
    13561 gccataatct taacaactga tttttaaagt ttattatctt aacaaagttc attatcttat
    13621 ataatattta aatgcatagg cttctttaat ttaaccatct aaatatttaa ttaaatatta
    13681 ttatatgtaa tataactaat ttttaaatga atgtatagga gctggaatcc ttcctcctct
    13741 ttgagtaaat aattcagatg aatttgtatt tataggcatt actggtgctc gtcctaaaag
    13801 tcctccaaat tctaccatat cacctacatc acatcctggg gctggtataa ttctaacggc
    13861 tgtagtctta ttattaatca tacctattgc tgcctcatca gctatcatgg ctccaagcgt
    13921 acctgctgta gtatttcctg gcacagctat catatcaaga cctacagaac aaatagcagt
    13981 cattgcttca agcttatcta tacttaaagc acctttaata acagcatcaa tcatacctgc
    14041 atcttcagat actggtataa acgctccact aagtcctcca acatgtgaac atgccattac
    14101 tccacctttt ttaacagcat catttaaaag tgctaatgct gctgtagtcc catgagtacc
    14161 aacaacttca agacccattt cctctaatat atttgctacc gaatcaccaa tagcaggtgt
    14221 aggagctagt gataagtcta ttatcccaaa tggaacatcc aatctactag aagcttcctt
    14281 tgccacaagt tgtccaactc ttgtaatttt aaatgcagtc tttttaactg tttctgaaac
    14341 aacatcaaaa ggctctccct ttactttctc taaagctctc ttaacaacac cagggccact
    14401 tacaccaaca tttataattc tatcagcctc accaacacca tggaaagctc ctgccataaa
    14461 tggattatct tctactgcat tacaaaatac tactaatttt gcacaaccaa aaccttttgc
    14521 atcttttgtt aattctgcag tttcttttat gatttctccc atttctttca ctgcatccat
    14581 atttatacca catcttgtag aacctacatt tactgaagca catactttat tagtacttgc
    14641 tagagctttt ggaattgact taattagtat cttatcacct tttgtatatc ccttttgaac
    14701 taatgctgag aatccaccaa taaaatctat acctaaatcc tctgcagcct tatccaatgt
    14761 ctgtgcaaac tttacatagt cattttcatc tgttgcacct gcaataatgg aaattggagt
    14821 tactgatacc cttttattta caattgggat accaaattca cgctctatat cttttccaac
    14881 cttcaccaaa tctttagcag aactcataat cttattgtat attttttctc tagctttatc
    14941 tccatcaggg tctatacaat ctaataaaga aatccccata gttatagttc ttatatccaa
    15001 ctgttcttca tctatcatct ttattgtttc aagtatattt cctacattca ctattatcac
    15061 ctccccttac tgtctactac agattatgca ttgaattaaa tatttcttct cgctgaattt
    15121 ttacatccac tgataatttc ttacctcttt caaccaagtc ctcaaccgtt ttctcaaaag
    15181 aatttaatga tttctctaaa tcaactatca taatcatagt aaaataatcc tgtaatattt
    15241 tttgtgatat atctactatg ttgatatttt gcttgtataa ttcatcacta attcctgcca
    15301 ctatacctgg tttatccttt ccaattactg ttaaaattgc tttcataata atatcctccc
    15361 ttattttttt ataaatctta tatttttaca aattgaacta tgtgttaaca tatatttcat
    15421 ttatttgtta gtaaaccact ttttatgtat atatttaata tattttaata cctatacatt
    15481 catttgtcaa ttttttttca atattctatt atttttatac tatttagaaa attcatatat
    15541 tatatatgtt ttctcaacaa taaatacagt tttcataa
//
LOCUS       DAELLV010000076        15514 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.076, whole genome shotgun sequence.
ACCESSION   DAELLV010000076 DAELLV010000000
VERSION     DAELLV010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15514
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.076"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            260..2494
                     /locus_tag="KPD58_001868"
     CDS             260..2494
                     /locus_tag="KPD58_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG0265638.1"
                     /translation="MQNHNYIKMLNKKVEVLSKELDSRNFKLKTLNEIDFFSKIPNRE
                     FMYQHIDDCISKNIHIGIMLLNIDNFKLFNDIYGYLVGENLLITLTQNLLHFIPESKG
                     IVAYLKGEGFLIVLPNVSNKELNNMGATIIESARELKIKLPNEKEIYSNVTFSIGSTI
                     WNGEPNMTTLMLFNQVYSALKKAKSEGKNRYILFDFKDNSFYNKLENDKEFLSEISHV
                     LQTKQIEVFYQPLYNIKNNTIVGCEALARWRHPIKGLLTPDKFIPLFEQFGLITELDL
                     HLFEENCKNIRRWIDNNDVFVPICCNFSRLHFFNPNFPSMLKGITEKYSVSTSNFSIE
                     ITENILIENTNTIIQQLAELRSYGFSVSMDDFGSGYSSFGMLQNLPFDIIKIDRIFLS
                     HNLNDFKNTTILYSIINIARALGMLTVCEGVETEQHVEFIKSINCDIAQGYYYAKPME
                     CKEFEKLLSKENNSEKMFIKYSDIGKSLIEKIFDGFFIMQDMKKLRANVTEDIQWFDF
                     YNKTEITSFPKVIEHFEKHIKGKKFSILYKSITPHNTESDSLLISGEAVLSDEASEHQ
                     KYSNFYFSANCIISENKLLLAKLHLDLIKTAGYINEFESYSIPKMTNTHLDESNILSQ
                     FYSMLPVGIIRYDLFGDMIITYMNEEMFDIIGYTKEQFFGEMGGNLRAIVHPDDLDFV
                     YKKSLDMIDTNKIEPFLYRFIKRDGQIVTVMYTQCNLSAIDGRSIAQGMYVDVNKIKN
                     LTKI"
     gene            2525..3358
                     /locus_tag="KPD58_001869"
     CDS             2525..3358
                     /locus_tag="KPD58_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG0265639.1"
                     /translation="MFKEIFLRTFPGFLIIRDSNYRIIFINDNLKNLIKSYMQDNPLG
                     MTNIEIAKKLPDNIAKFFTDSHNIQLDWEKNYPYDKISNWILEFKKDTTSYWNVLEYK
                     VDVDEKTYIITMANDITKLYEENKRNLHYSITDPLTGAYNRKYLNDRFDIFIGDYIVL
                     IDLDNFKMINDYEGHNVGDKILCDFVSLLNKELINSTSIIRLGGDEFIVIFSSDVDKN
                     YVYSQLEALRENFLKVFSKYKYLSFSYGVDTVKRNLKLTIVELDKKMYKNKKKFDKND
                     Y"
     gene            3598..5331
                     /locus_tag="KPD58_001870"
     CDS             3598..5331
                     /locus_tag="KPD58_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG0265640.1"
                     /translation="MSILLKKAPKLAKHIITSFYINRDIDEVLKYLCENVTWIGPGEQ
                     EFLTSFNEIKNYFYAGQDEIPSCDINNDIFETVSEDENRCMVLGRYTVRTKENTQMIL
                     EVNQRCTFEIIEDRGKLLVKHMHISNPYGEMQLDEYFPTKIGTQSYDYLQRLLKEKTE
                     VIEMITNNINGGLKGSNDDNTYSFFYVNEGLPKILGYTYDEFMEMSGGSAVGAVYPPD
                     LPKALEDCERCFAKGPTYSSEYRIRKKDGTLMWVLDSGMKSLNSDGIVKINSIITDIT
                     QLKNIESELKLERERYRIALQNITDIMFEYDIENDTLIKYQRVEIDKKIELENFETKN
                     YSKLLESGKIIHLDDIGKLLEVLHGNLRETIEIRQVNSLTKNEWRWIRVQCSVIYDSD
                     HNPIKTIGVLKDITEDKSKLESLINQAQRDPLTQLYNQRVSQNLIEEYLCSSDSKNND
                     ALLVIDIDDFKTVNDTFGHLEGNEVLVAVSKILLHNTHDKDIVARIGGDEFTIFIKSL
                     TKDLIIKITNDILNDASKIKVKDNHKITLSIGIAFTDDSTKLYKDLFSKADKALYLSK
                     ADGKNCYSTYE"
     repeat_region   5723..6017
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=5792..5820
                     /rpt_unit_seq="atttacataccacttagttaatataaaac"
     gene            complement(6547..6651)
                     /locus_tag="KPD58_001871"
     CDS             complement(6547..6651)
                     /locus_tag="KPD58_001871"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBG0265641.1"
                     /translation="MDDYIYYYNNFRYQNRLNSMSPFEFRKYLSTERA"
     gene            7276..7521
                     /locus_tag="KPD58_001872"
     CDS             7276..7521
                     /locus_tag="KPD58_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265642.1"
                     /translation="MNQKKIKISIKMVIIGIFAVIIAFVLSRLFVEYIDELLRSNWQY
                     QLFESESSYIVFANCIMFSNILLEIFLIYICRKFRKI"
     gene            complement(8274..8420)
                     /locus_tag="KPD58_001873"
     CDS             complement(8274..8420)
                     /locus_tag="KPD58_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265643.1"
                     /translation="MVFETFDKTIELYPNAKPIFHSDIGFRLDDESFEEYLGRVFRKR
                     CKGI"
     gene            8516..9165
                     /locus_tag="KPD58_001874"
                     /pseudo
     CDS             8516..9165
                     /locus_tag="KPD58_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429361.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase family
                     protein"
     gene            9706..11061
                     /locus_tag="KPD58_001875"
     CDS             9706..11061
                     /locus_tag="KPD58_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG0265644.1"
                     /translation="MDTKISINENMSLGKRFFKYLAPSVVAMWVFSLYTMVDGIFVSK
                     GVGELALAAVNISMPFINFIFAVSLLFSTGASTIIAIYLGKKDIKSANEVFSFNLVSI
                     IILSIIILAITFFNLDRLALFLGATESTIGMVKDYLGIIIFFNGFFIVSYSLEVIIKT
                     DGFPILATVGVIISALTNIILDYLFVIEFGWGVKGAGIATGLSQVFSTIFFLIHFLRK
                     NSTLNFSKFRIDFKTLRKIVFIGFPDSTTELSCGIVVLLFNLSLTKYIGENALIYYSV
                     INYINTLVLMTMMGITQGMQPLTSFYYGAGNIDNVKKLLKMGIKATIIASVAVFAICM
                     AFSGPIVSLFIHPEETMLFNEGVRVFKIFSISFLLVGINVIISGFFVSVEKPSISTVI
                     SLGRGLVIVVLSLISMILIFGGQGIWMTTIVSEFICLILSLVFLKKNFSTLDSNLNKV
                     A"
     gene            complement(11179..11847)
                     /locus_tag="KPD58_001876"
     CDS             complement(11179..11847)
                     /locus_tag="KPD58_001876"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HBG0265645.1"
                     /translation="MKEVLKLADEKFVMSFSGGKDSILALNRMLKKGYKPVALLTTIS
                     EEHGKSWTHNLEYNMLKQVSSNIGLPLLVAECGVEGYEESFERALIKAKNMGATICAY
                     GDIDIESHRKWDTDRCEAVGMKVDLPLWQESREDLVYEFIDSGFCSVVTKVNLKHLGE
                     EFLGKKLTRELVEKIKNAGADPCGEHGEYHTFVVDGPIFKKPVEYEVKGTLIKDGYGY
                     LEIK"
     gene            complement(11914..12342)
                     /locus_tag="KPD58_001877"
     CDS             complement(11914..12342)
                     /locus_tag="KPD58_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBG0265646.1"
                     /translation="MTKLWAIIFALLAGGSTALEAFINGELGKSTTALVATFISLTVG
                     AIFFLASIIVTGDLKCLVSINGFNYKLLLGGVFGGLIIYFTVKAIPNLGVSNTLILTL
                     VSQVLVGFFIDSVILGQETMHLYKYIGVILLILGTFFIVN"
     gene            12449..13042
                     /locus_tag="KPD58_001878"
     CDS             12449..13042
                     /locus_tag="KPD58_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBG0265647.1"
                     /translation="MNFCYNCSIDFGGDFMYTNFNIDFNNFNKKENAIKFMLMGVLLI
                     ILGLLCLTFKTLGIKLISWTFGIALLFFAYLNLKNINELKRYATKEEIKPSINIQWVL
                     IIACILLFVFPQKIQSIFSLFLGFYLIFNQLVALVNSKNNPYSKFTTWNIVKILFGIC
                     LILSPLFLSRFIVSIMSFFIILFGLVLFFSGNTARKY"
     gene            13161..14096
                     /locus_tag="KPD58_001879"
     CDS             13161..14096
                     /locus_tag="KPD58_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893027.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBG0265648.1"
                     /translation="MDKFKYAFDNKRYHTWNYYLRNTFGEKVFKVSINAGFTCPNIDG
                     SLGYGGCTYCSKEGSGDFAGNPKDNLISQFYNIKEMMLKKWPHAKYIGYFQAYTNTYA
                     PLEVLKEKYETILELEDVIGLSISTRPDCLPDDVVEYLSELNKRTNLWVELGLQTIHD
                     STSKIINRGHDYKTFVEGVEKLKSKNIKVVVHIINGLPGEDYNMMMETAKAVGKMGVD
                     GIKIHLLHVIKDTPMEKMLQNGMLTLMEQDEYINLVCDQLEILPETMIIHRLTGDGKR
                     DELVGPIWSLKKWEILNQIDDTLKARNSYQGCKFV"
     gene            complement(14368..15312)
                     /locus_tag="KPD58_001880"
     CDS             complement(14368..15312)
                     /locus_tag="KPD58_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBG0265649.1"
                     /translation="MGEKSKGYVFIAIAGLLWATLGLFGKFLMGNGLTSEQVAFTRLF
                     FGFIVLGVYSSIRTPQILKISKKGIIYSVIIGIICQAMFNLCYFKAIDIAGVSIAAVL
                     LYTSPLFLAIFSKICYKENITRSKLFSLILCFIGAIMAVTGGRLDFQGLNAFGLLLGV
                     LSAIAYALMPTISKNALKEFSSSTILVYSFLFGAIFMIPSSRPWEILNYAKDLDVLSC
                     MLMLGIVPAALAYIFYAAGISKGVELSVAGVVASVELVGSVIIGCTILGESFSLGKLF
                     GVMLMLISAVVALNLSYDEIRIFYKSNKLKQIEKTESI"
BASE COUNT         5891 a         2030 c         1965 g         5628 t
ORIGIN      
        1 gaaaaaatat gatataattg cattcaaaaa ggaaatcttt caatatacta gaatttaagc
       61 attaactaat tttatctaat attattacta aactaaaaaa tatttgtatt tgtagcattg
      121 gagcaagtaa cttaatgtat attcatttta aatacttatt taaaatttag catttaaatt
      181 atttacaaat taacatttaa atacttctag cataaataca gttgtcatta aaatttaaag
      241 tatcttatgg aggaatacca tgcaaaatca taattatata aaaatgttaa ataaaaaggt
      301 agaagtatta tctaaggaat tagatagtag aaattttaaa ttaaaaacct taaatgaaat
      361 agattttttt tctaaaatac caaacagaga atttatgtac caacatattg atgactgtat
      421 ttcgaaaaat atacatatag gaatcatgtt attaaatatc gataacttta aattatttaa
      481 tgatatatat ggatacctag tcggagaaaa tttattaatt actctaactc aaaacctatt
      541 gcactttatt ccagaatcaa agggaatagt cgcttattta aaaggagaag gttttttaat
      601 tgtacttcca aatgtatcaa ataaagagtt aaataatatg ggtgcaacta ttatagagag
      661 cgcccgagaa ttaaaaataa aacttcctaa tgaaaaagaa atatactcca atgttacatt
      721 tagtatcggc agcactatct ggaatggtga accaaacatg actactttga tgcttttcaa
      781 ccaagtatat agtgctttaa aaaaggctaa aagtgaaggt aaaaatagat acattctatt
      841 tgattttaaa gataatagtt tttacaataa attagaaaat gataaggagt tcttatcaga
      901 aatatcacat gttctacaaa ctaaacaaat tgaagtattt taccaaccac tttataatat
      961 aaaaaataat actatagttg gatgtgaggc acttgcaaga tggagacacc ctattaaagg
     1021 acttcttact ccagataaat ttattccttt atttgaacag tttggtctga taacggaatt
     1081 agatttgcat ttatttgaag agaactgtaa aaatataaga agatggattg ataacaatga
     1141 tgtttttgtt ccaatttgtt gtaacttttc tcgtcttcat ttttttaacc ctaattttcc
     1201 aagtatgcta aaaggtatta ctgaaaagta cagcgtctct acatcaaatt ttagcattga
     1261 aataacagaa aatatattaa tagaaaatac aaatacaatt attcaacaac tagctgaact
     1321 tcgttcttat ggtttttctg tatcaatgga cgattttggt tctggatatt catcttttgg
     1381 tatgttgcaa aatctacctt ttgatataat caaaattgat aggatatttt tatctcataa
     1441 tttaaatgat tttaaaaata caaccatttt gtactctata ataaacattg caagagctct
     1501 tgggatgctt acagtatgtg aaggtgttga aacagaacaa catgtagaat ttattaaaag
     1561 tataaattgt gatatcgccc aaggttatta ttatgcaaaa cccatggagt gcaaagaatt
     1621 tgaaaaatta ctatctaaag agaataattc agagaaaatg tttataaaat attctgatat
     1681 aggaaaatct ttaatagaga agatatttga tggatttttt attatgcaag atatgaaaaa
     1741 actaagagct aatgtcactg aagacattca atggtttgat ttttacaaca aaactgaaat
     1801 tacatctttt cctaaagtaa tagagcattt tgaaaaacat attaaaggta aaaaattttc
     1861 tattctttac aaaagtataa ccccacataa tactgaatca gattcattat taattagtgg
     1921 agaagccgtt ttatcagatg aagcttctga acatcaaaaa tatagcaatt tctactttag
     1981 tgcaaattgt ataatttcag aaaataaatt actcttagca aaattacatt tggatttaat
     2041 taaaactgct ggctatataa atgaatttga atcatattca atacctaaaa tgacaaatac
     2101 acatcttgat gaaagtaata ttttatcaca attttattca atgttgcctg taggtataat
     2161 tcgctacgat ttatttggtg atatgattat tacttatatg aatgaagaaa tgtttgatat
     2221 tataggttat acaaaagaac agttttttgg tgagatgggc ggaaatttaa gagcaattgt
     2281 tcatccagat gatttagact ttgtttataa aaaaagtttg gatatgattg acacaaataa
     2341 aattgaacca tttttatata gattcataaa acgagatggt caaattgtca cagttatgta
     2401 tacacaatgt aacctttctg caattgatgg aaggtcgatt gcccagggta tgtatgtaga
     2461 tgtaaataaa ataaaaaatt taacaaaaat ctagtacaaa gtattttatt ttggagaaat
     2521 taatatgttt aaagaaattt ttttaagaac ttttccagga tttctaatta taagagattc
     2581 taactacaga attatattta taaatgataa tttaaaaaat ttaattaaaa gttatatgca
     2641 agataatcca cttggaatga ctaatataga aatagctaag aaactccctg ataatattgc
     2701 taaatttttt acggattctc acaatattca attagattgg gaaaaaaatt atccttatga
     2761 taaaatttca aattggattt tagagtttaa aaaagatact acaagctatt ggaatgtttt
     2821 agaatataaa gtagatgttg atgaaaaaac ctacataatt actatggcca atgatataac
     2881 aaaactctat gaagaaaata agagaaatct tcactactca attactgacc cattaactgg
     2941 agcatataat agaaaatatt taaatgatag atttgacata tttataggag attatattgt
     3001 actaattgac ttagataatt tcaaaatgat aaatgactat gaaggtcata atgtaggaga
     3061 caagatttta tgtgattttg tttcactttt aaataaagaa ttaataaatt ctacttctat
     3121 tataagatta ggaggagatg aatttattgt tatattctct agtgatgttg acaaaaatta
     3181 tgtttattca caactagaag ccttaagaga aaatttttta aaagtattct ctaagtataa
     3241 gtatctttca tttagttatg gtgttgatac agtaaaaaga aatttaaaat tgacaatagt
     3301 tgaattggat aaaaaaatgt ataaaaacaa gaaaaaattt gataaaaatg attattaata
     3361 ttgttatttt aaaataaatt aaaattatct ttcttttttt aaacttgtac attagtttat
     3421 aaatatgtat ctatacaaga tattgacatt aagtactgaa gcatatattg tttcagttct
     3481 tttttgttaa aattaactta tttaacaaag atatcatagt ttaacaaagc ttttaattta
     3541 aatatattga tgtataatat aattaaaata taatagcaaa aagaaaggag gaatatcatg
     3601 agcatactcc taaaaaaagc acccaaatta gcaaaacata ttataacaag tttttatata
     3661 aatcgggata ttgatgaagt attaaaatac ctttgtgaaa atgttacatg gatcggacca
     3721 ggagaacagg aatttctcac ttcttttaat gaaataaaaa attattttta tgcaggtcaa
     3781 gatgaaattc cttcttgtga tataaataat gatatatttg aaactgtaag tgaagatgag
     3841 aatagatgta tggtattagg aagatatact gttcgaacta aagaaaatac tcaaatgatt
     3901 ttagaggtaa atcaacgttg tacatttgag ataattgagg atagaggaaa attattagta
     3961 aagcacatgc acatatcaaa cccttatggt gaaatgcaac ttgatgaata ctttcccact
     4021 aaaattggaa ctcaaagtta tgattatttg caaagattac taaaggaaaa aacagaagtt
     4081 attgaaatga ttacaaataa tatcaatggt ggcttaaaag gtagcaatga tgataataca
     4141 tactcatttt tctatgtcaa tgaaggattg cccaaaatat taggatacac atatgatgag
     4201 ttcatggaaa tgagtggtgg ttcagcagtg ggtgctgtat atccaccaga cttacctaaa
     4261 gcactagaag attgtgaacg atgttttgca aaaggaccta catattcatc tgaatataga
     4321 atccgaaaaa aagatggaac attaatgtgg gtattagatt ctggaatgaa atcattaaat
     4381 agtgatggca tagtaaaaat taatagtatt attactgaca taacccaact aaaaaacata
     4441 gaatctgaac taaaattaga aagagagcga tatcgtattg ctttgcaaaa tattacagat
     4501 atcatgtttg agtatgatat tgaaaacgat acccttataa aatatcaacg tgtagaaatt
     4561 gacaaaaaaa ttgaattaga aaattttgaa acaaaaaatt actccaaact attagaatct
     4621 ggtaaaatta ttcaccttga tgatattgga aaactactag aagtacttca tggaaatctt
     4681 cgtgaaacaa ttgagataag acaagtaaat tcattaacta aaaatgaatg gagatggatt
     4741 cgtgttcaat gttcagttat atatgatagt gaccataacc ctatcaaaac aataggcgtt
     4801 ttaaaagaca taactgaaga taaatctaaa ttagaaagct taattaatca ggctcaacgt
     4861 gacccactaa cacaattata taatcaaaga gtatcacaaa atcttattga agagtatcta
     4921 tgtagttctg attccaaaaa taatgatgct ctattagtta ttgatattga tgattttaaa
     4981 actgtaaatg atacttttgg gcatttagag ggaaatgaag ttttagttgc tgtatcaaaa
     5041 atactattac ataacacaca tgataaagac atagttgctc gaattggtgg agatgaattt
     5101 acaatattta taaaatcatt aacaaaggat ttaataataa aaattactaa tgatatttta
     5161 aatgatgcaa gtaaaataaa agtaaaggac aatcataaaa taacattaag tattggtatt
     5221 gccttcactg atgattcaac taaattatat aaagatttat tttctaaagc tgataaagct
     5281 ctttatctat caaaagcaga tggcaaaaat tgttatagta catacgaata ataaagctat
     5341 ttatacgacc aagaatgaac ctttaaattt aatttcatag tctaacaatc aaagagttga
     5401 agctaatctt atagctagtc aactagttcc tagaaaaaat ttaaacaata tatttattga
     5461 tactgctcat ttttctaaag agcaaattta tataaaaaat taaactttta tgtacataca
     5521 gatactaatg ataaacagtt atcattagta tcttctatct taaaacaaaa tgataattta
     5581 tctatataaa aaagtgcttt gttgacagag ttcacttttt tatataggta cagaatttaa
     5641 gtataaatac atgttttatt ctatacattt tagttaatgt aaaatttctt gattgtactc
     5701 tctaactttt caacattata ctatttatat accacttagt taatataaac cccttagctg
     5761 gttctagtgc tttttctatg attttagtca tatttacata ccacttagtt aatataaaac
     5821 caatttagat gatgtatatg tgcaaaagga agaaataatt tacataccac ttagttaata
     5881 taaaacggtc actacaactt atacttccag tctaacgatt tgaatttaca taccacttag
     5941 ttaatataaa acatattttg gatttattaa tataaacata attaaaaaat ttacatacca
     6001 cttagttaat ataaaacccc aaaataaact gggcattttc aatacttaca catatgcaac
     6061 tctcttaaat ttgcagtgaa ccatgagtag tgcaaatgat aaagcttatc atataccctc
     6121 aacaccttat atttcaagcg ttaagctcta ctttgtaaca aatatcgctc actgcaaaac
     6181 tatttacatt tttattatat cataaatata ttatttttga atatctatac caatttgtgg
     6241 tataataaaa gcaagaagaa ctacaatcta tttagacgta gagtgaagtt caaatactaa
     6301 tgtttattct ttttgaactt gatttttagt tcaaaactaa agtcactcct gcctgtgtga
     6361 actatacccg ctgttttata cagttaaaac atgtaaaaat agttataaag tactagtgta
     6421 actcattagt actcttttta ttatattcat ataattaata tctatatttt aaagtttact
     6481 atctaaaaaa gagcacgtca aataaaaccc tagatattta atttttttag agatacttaa
     6541 aataaattat gctctctctg tacttaaata tttcctaaat tcaaatggag acatagaatt
     6601 taatctattt tgatatctaa aattattata gtagtatata taatcatcta ttgctttaac
     6661 taattattca tatgtatcaa atttccttaa atagtacatt tctaccttta aaattcccca
     6721 aaatccttcc ataggagcat tgtcaaggca acgaccaact cttgtataat atatgtatat
     6781 ctaacaccat taagaaatat atattttata atatatattt cttcatttaa tgcacttaga
     6841 aaagcaaaga aaatacagat tagtacactt tttagagaaa aataaaatta atacatctaa
     6901 aatattaaaa aatactttag ataatctaaa gacacatctc aaagttttta actaaattat
     6961 aaaactaatt acaaatgcta tgtaacgata ataaataaaa aatttacttc gcttaagcga
     7021 cgtgtcgttg aaatatttca tgttgccaac tggttcgtaa gttgataaga aaagaacact
     7081 aattatttag agttcttttc ttagctacaa gtggattata atacttttaa atctgtgaga
     7141 aaatcatata taataatgat atgaacaagt aaagaaaata caaaaatgac aaaattataa
     7201 ataaaatatt ataaaatcat aaggtcataa ttgttatatt gtagaaacaa agtaaagaga
     7261 gagggtggag aataaatgaa tcaaaaaaaa ataaagatta gtattaaaat ggtaattata
     7321 ggtatatttg ctgtaattat tgcttttgta ttatccagat tattcgttga gtatattgat
     7381 gaattattaa ggtctaattg gcaatatcag ctatttgaaa gtgagagttc atatatagta
     7441 tttgccaatt gtattatgtt ctcgaatata ttattagaaa tatttttaat atatatttgt
     7501 agaaaattta gaaaaatcta atatggtcaa cgtatttcga atcgaatact tcatgattat
     7561 ttagtctatt gtgctagtta aagtttaatt cccatataaa gtaaattgat tttagtaaaa
     7621 caaatcaccc ctaagggtta gtttatggat aagtcatcca aatattaact ctttaatttt
     7681 tgctatagat tcatctttgc tcataacttt acttataaaa aatgaaatat tataagctag
     7741 aacttttatt ctaattctag tcataaatcc tagcatagag ttactttttt tatattataa
     7801 ataaaactat acaacaaaga taatatttag taaaaataac tttcatttta actaaactta
     7861 aaattcaatt actctctaaa ttgacgttta ggaattagtt caaaataaaa aagagtgtat
     7921 atattgaaat tactacattt caatatatac actcttttta aatttctcag ttttccatgg
     7981 aactttactt tatttatttc tgtattagtt gtatttttta tgcatttttc tttaaaatca
     8041 tattttctga ttcaactctt atattataaa atttctttac taaattattc tctttttcta
     8101 tatcttcata tatatttttt aatcgccttt tacctaatct tttatctaac attactaatg
     8161 atttaactaa tatattatat gattttatcg catcttctat tgagatattt aaacatatat
     8221 ttactgcttc ataaaaatca aattcattaa aaaatccatt tttatttaca atcttatatc
     8281 cctttgcatc tctttctaaa tactcttcct aaatactctt caaaactttc atcatctaat
     8341 ctaaatccta tatcactatg aaaaataggt tttgcatttg gatatagttc tattgtttta
     8401 tcaaaagttt cgaataccaa gttattatta ttcgattttc caacagtcca ctataaacaa
     8461 taataagttt gttgctccaa ataatgtaac acttgtagaa agatgttaca gatatataaa
     8521 agatagagaa tgtagttttt ctcctaatac tattagaaat agaaaaaata tgattaaaaa
     8581 tcaaattgaa gttttcttta aagatatgaa attaatagat ataactcctt ctattttaca
     8641 gtcttatata aataacatat acaatgagca catgttaaat tcaacaaaaa accaagtcga
     8701 ttttataaaa tcagtattga aagaatctta tagattaaaa gaaatatcag aaaatatttg
     8761 tgattttgtt acaaccccaa tcaaaaaaaa tagttcaact tctaagcttt atacaaaaca
     8821 aaaagctcaa ttattattag aaaaatcaaa aaatattcct ataggtatac caattttttt
     8881 gatgctaact cttggattac gttttggtga agctgtatca cttatatggt cagatgtaga
     8941 ccttgataaa aaatctcaca tgtaaatcaa acattagttt atgtatacaa taaaataatc
     9001 ttcaaaggcc ctaaaacccc tagattcaaa agaagattat ttgttcctga tgaacttata
     9061 agtttgttta aaggggaaaa attaaaacaa aataagttaa aattacaggg tatattgaaa
     9121 aatgaatttg atttgatatg cttaaataaa agattcaacc cgtgagttca accaactttt
     9181 gttgttcatt ttaaaaaact attaagagat aataatccta gagatataaa agtacatgaa
     9241 ttaatacatg caaatgcgac tttgatatta ctttcaggta ctaacataaa agctatttat
     9301 aaaatattag tacatgcaga tataaaaata gctatgaata tatattccat attctcactt
     9361 attagatgaa atgaataaag aaacatctga aaatataagc aaaatactat tcaaataatg
     9421 ttcagtttta tgtcaattaa atgtcactca aagtgctttt tttccatttg tcagttaaat
     9481 gtcacttaaa gtaccttttt cctatttgtc agttaaatgt cagtaatata taaataagat
     9541 atatttataa gacccttaaa atcactatta tttaataaaa tttataatta tatactattt
     9601 gctgttgaca ttggtattat accatagttt atcatttata ttagaataaa attattaatt
     9661 aatagtttcg taattttata atagatttac agggagtgat aataaatgga tacaaaaata
     9721 tctataaacg aaaacatgag tctaggtaaa agatttttta aatacctagc accatctgta
     9781 gttgcaatgt gggttttctc actatataca atggttgatg gaatatttgt aagtaaagga
     9841 gttggagaat tagctttagc tgctgtcaat atatcaatgc cttttattaa ttttatattt
     9901 gcagtttctc tattattttc aactggtgca tctaccataa ttgcaatcta tctggggaaa
     9961 aaagatatta aatctgctaa cgaagtcttt tcatttaact tagtatcaat aattatatta
    10021 tcaataatta tattagctat aacttttttt aatttagata gattagcact atttttagga
    10081 gctacagaat ctacaattgg tatggttaaa gattatttag gaataataat attttttaac
    10141 ggattcttta tagtgtctta ttctttagaa gttataataa aaactgatgg ctttccaata
    10201 ttagctactg ttggtgtaat aatatctgct cttacaaaca tcatacttga ttacttgttt
    10261 gtaattgaat ttggatgggg tgtaaaaggt gctggtattg caactggttt gtctcaagtc
    10321 ttttctacta ttttcttctt aatacatttt ttaagaaaaa actctacttt gaatttttca
    10381 aaatttagaa ttgattttaa gacacttaga aaaattgtat ttataggttt tcctgattca
    10441 actactgaat taagttgtgg tatagttgtt ttacttttta acttaagtct tactaaatat
    10501 attggagaaa atgctcttat ttattatagt gtcataaatt atataaatac attagtactt
    10561 atgactatga tgggtataac tcaaggtatg caaccactta ctagctttta ttatggtgca
    10621 ggaaatatag ataatgtaaa aaaattatta aaaatgggga taaaagccac aataatagca
    10681 tcagttgctg tatttgcaat ttgtatggct ttttcaggac caatagtctc tttatttata
    10741 catccagaag aaacaatgct attcaatgaa ggagttcgtg tatttaaaat attctctata
    10801 tctttcttat tggtaggaat taatgtaata atttctggat tctttgtatc agtagaaaaa
    10861 ccatcaattt ctactgttat atctcttggt agagggcttg taatagttgt tttaagtcta
    10921 atttcaatga tactaatatt tggtggacaa ggaatatgga tgactactat tgtttctgag
    10981 tttatatgtc ttattttatc attagttttt ctaaaaaaga atttttctac attagactct
    11041 aatctaaata aggttgctta gatgataaga gtgacaatat atgaaaatat ctaaataaaa
    11101 aatacctatt atacaatctt gcaaatatta aacctttgca aaaattagga ttatataata
    11161 ggtattttat ttatgtaatt attttatttc taaatatcca tagccatctt ttattaaagt
    11221 tccttttact tcatattcta caggtttttt aaatattgga ccatctacga caaatgtatg
    11281 atactctcca tgttctccac atgggtctgc accagcattt tttatcttct ccacaagttc
    11341 tctagttaat ttttttccta aaaattcctc tcctaagtgc tttaagttta cttttgttac
    11401 aacactacaa aatccactgt ctataaattc atatactaaa tcctctctac tctcttgcca
    11461 taatggtaaa tctactttca taccaactgc ttcacatcta tcagtatccc attttctatg
    11521 actttctata tctatatctc catatgcaca tatcgttgct cccatgtttt tagcttttat
    11581 taaagccctc tcaaagctct cttcatatcc ttctacgcca cactcagcaa ctaataaagg
    11641 taatcctatg ttgctactta cttgcttaag catattatat tctaaattat gtgtccatga
    11701 ctttccatgt tcttcactta tagttgtcaa gagtgccaca ggcttatatc cttttttcaa
    11761 cattcgattt aatgctaata tactatcctt accaccacta aatgacatta caaatttttc
    11821 atcagccaat tttaacacct ctttcatcta actttgtttt attaacataa ttataccaaa
    11881 tttttgagtt gcatagcaaa ttatattaaa ttgttaattc actataaaga atgtccctaa
    11941 tattaaaagt ataaccccta tgtatttgta taaatgcatt gtttcttgtc ccaaaattac
    12001 gctatcaata aaaaatccaa ctaaaacttg ggatacaaga gttaatataa gtgtatttga
    12061 tactcctaaa tttggaattg cctttactgt aaaatatatt attagacctc caaatactcc
    12121 tcctaataac aacttataat taaaaccatt tatagatact agacacttta agtcacctgt
    12181 aactattata cttgccaaaa aaaatattgc tcctacagtt aagcttataa aggtggccac
    12241 taatgctgtt gtactttttc ctagctctcc atttataaaa gcttcaagag ctgtagaacc
    12301 acctgctaat aatgcaaata ttattgccca taattttgtc atggacaaac ctccttctat
    12361 ataattctat taatagaagt ttatgataca acctattaat taattactaa gtatagttta
    12421 atttttatac actaggcttt atttggatat gaatttctgt tataattgtt ctatcgattt
    12481 tggaggtgat tttatgtaca caaattttaa tatcgatttt aataacttta ataaaaaaga
    12541 aaatgctatt aaatttatgt taatgggagt attactcatt atattaggat tattatgcct
    12601 tacatttaag acattaggta taaaattaat ttcttggact tttggtattg cacttttatt
    12661 ctttgcatat ttaaatttaa aaaatattaa tgagttaaaa agatatgcta ctaaagaaga
    12721 aattaaaccc tctattaata tccaatgggt tctaataatt gcatgtatat tactttttgt
    12781 atttcctcaa aaaatacaaa gcatattttc attattttta ggattttatt taatctttaa
    12841 tcaactagta gcacttgtaa atagtaagaa taatccttat tctaaattta caacttggaa
    12901 tatagttaag attctatttg gtatatgcct tatattatct ccactttttt tatctagatt
    12961 tatagtatcg ataatgtctt ttttcataat attatttgga ctagttctat ttttctctgg
    13021 aaatacagcg agaaaatatt aattattttc tcgctgtatt tttatattac ttaaaataga
    13081 atttgtttaa gttatctatt tgtggtaata tgttttttgt acaagctcag cgtacaattt
    13141 tattagaaat aggtgatttt ttggataaat ttaaatatgc ttttgataat aaaagatatc
    13201 atacttggaa ttactatctt agaaatactt ttggagaaaa agtttttaag gtatctataa
    13261 atgcaggatt tacttgccca aacattgatg gaagtcttgg ttatggcgga tgtacctatt
    13321 gtagcaaaga aggctcaggt gattttgctg gaaaccctaa agataatctt atcagtcaat
    13381 tttataatat aaaagaaatg atgttaaaaa aatggcctca tgcaaagtat attggctact
    13441 ttcaagccta tacaaatacg tatgctcctc ttgaagtttt aaaagaaaaa tatgagacta
    13501 tattagaact tgaagatgtc ataggtcttt caatatccac aagacctgac tgtttgccag
    13561 atgatgttgt agaatactta tctgaactaa ataaaagaac caatttatgg gttgaacttg
    13621 gacttcaaac aattcatgat tctacatcta aaattataaa tagaggtcat gattataaaa
    13681 catttgttga aggtgttgaa aaactaaagt ctaaaaatat aaaggttgtc gtacacatta
    13741 taaatggtct tcctggtgaa gattataata tgatgatgga aacagcaaag gcagttggta
    13801 aaatgggtgt agacggtata aaaatccatc tattgcatgt tataaaagat actcctatgg
    13861 aaaagatgtt acaaaatggt atgttaactc ttatggaaca agatgaatat atcaatcttg
    13921 tctgtgacca attagaaata cttcctgaaa ctatgataat ccatagactc actggtgatg
    13981 gtaagaggga tgaactagta ggtcctatat ggagcctaaa aaaatgggaa attttaaatc
    14041 aaattgatga tactttaaag gctagaaatt cttatcaggg ctgtaagttt gtttaataca
    14101 aaataataac tatagaaatg gttatataaa tatagtcctt tctatagtta tttagtattt
    14161 acaaaaacaa aatattaatt tgtacattta tacaatatat aataaaaaat agatagtcta
    14221 aactgtatct acttatattt tatttgtact gtccacttta ttctataaaa tttataaata
    14281 gttatttttt aataccaaat ataatttggt ataatttttg ttggactcta aaatgtatac
    14341 caataaatcc tttttaagta tataattcta aatactttca gttttttcaa tctgctttaa
    14401 cttgttggac ttataaaata ttcttatttc atcataagaa agattaagtg ctactactgc
    14461 agatattaac atcaacatca ctccaaataa cttccctaag gaaaaacttt ctcctaatat
    14521 agtgcaacca attattactg aaccaactaa ttctactgat gccacaacac ctgcaactga
    14581 caactctaca cctttagata tacctgctgc ataaaaaata tatgcaagtg cagctggaac
    14641 aatgcctaac attagcatac aagataaaac atctaaatcc ttagcataat ttaagatttc
    14701 ccaaggtctt gaagaaggaa tcataaatat agctccaaat aagaaactgt atactaatat
    14761 tgttgaactt gaaaattcct ttaaagcatt tttactaata gttggcataa gtgcatatgc
    14821 tattgctgat aataccccaa gtaaaagtcc aaaagcgttt aaaccttgaa aatccaaacg
    14881 tccaccagtc actgccataa ttgcaccaat aaaacaaaga ataagtgaaa ataatttgct
    14941 tctagtaata ttttctttat aacatatttt tgaaaatatt gctaaaaaca gtggtgatgt
    15001 atatagtaaa actgctgcaa ttgatacacc cgcaatgtca attgctttaa aataacataa
    15061 attaaacatt gcctgacata taatacctat tattactgaa tatataattc cttttttgct
    15121 tatctttaag atttgtggtg tccttataga tgaatatacg cctaaaacta taaatccaaa
    15181 aaacaatctt gtaaatgcta cttgttctga tgtcagtcca tttcccatta aaaactttcc
    15241 aaataatcca agtgtagccc ataataaccc tgctattgct ataaatacat accctttact
    15301 tttttctccc attttaaatt cccttctatt tttatttagt caatttacac tcttcttttt
    15361 aagcaaaaac tatgccatct taaatcaata tatatcgact ctaaaaccct agtctttcca
    15421 ataatactaa taaattttca atttttttat atttattttt cacttacttt aagttgattt
    15481 agacttattt taattgtaaa atgagttata tata
//
LOCUS       DAELLV010000077        15429 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.077, whole genome shotgun sequence.
ACCESSION   DAELLV010000077 DAELLV010000000
VERSION     DAELLV010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15429
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.077"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            193..750
                     /gene="efp"
                     /locus_tag="KPD58_001881"
     CDS             193..750
                     /gene="efp"
                     /locus_tag="KPD58_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBG0265650.1"
                     /translation="MVSAGDFRKGVTFEKDGQPCLVVDFQHVKPGKGAAFVRTKYKNL
                     KTGAIREESFNPSEKFPKAVIDTRQMQYLYNDGELYYFMDQENFEQIPLNYEQVEDAI
                     KFLKENEVATIRFYQGQPFQVEAPNFAELEVTDTEPGIKGDTASNVTKAATVETGAVV
                     QVPLFINTGDKVKIDTRTGEYLSRV"
     gene            842..1297
                     /locus_tag="KPD58_001882"
     CDS             842..1297
                     /locus_tag="KPD58_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG0265651.1"
                     /translation="MKHLYEEVAYLKGLAEGLEISAESKEGKMIHKIVDALEVFADAI
                     VTLDEEQEELQDFVESIDEDLADLEEDIADMEEDLYEEDDDEDDEDFSYIEMECPNCG
                     ELVEIDEDLLYDDEVDVVCPDCKAVILSSEDDCDDDCTCGGCSSCDDRE"
     gene            1460..2170
                     /locus_tag="KPD58_001883"
     CDS             1460..2170
                     /locus_tag="KPD58_001883"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBG0265652.1"
                     /translation="MKISKKLLDNIQRFENVINYKFKNKEYILEALTHSSYSNENKKY
                     NFNERLEFLGDSVLGIVISDYLFNEEANLPEGELTKLRANIVCEDSLSEVANDINLGI
                     HMLLGRGEEATGGRHRTSILADAFEAVIAAIYLDGGFESARQFILHHMENIIYDSRKG
                     NIFRDYKTHLQEVLQGNGENNIWYRLIEEKGPDHNKRFVMEVGINDDVLGIGEGKSKK
                     EAEQLAAKIALKKKLWEK"
     gene            2224..3294
                     /locus_tag="KPD58_001884"
     CDS             2224..3294
                     /locus_tag="KPD58_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG0265653.1"
                     /translation="MKKRIIPIFVPHKGCPHDCIFCNQKKITGVSTDVTSEDARNIIE
                     ECLETIDKDADVEIAFFGGSFTAIDIDIQKSLLSVAKEYVEKGLVKDIRMSTRPDCID
                     EDILSMLKEYKTSIIELGVQSLDEKVLLDSIRGHQSEIVYKSSKMIKNSGIKLGLQMM
                     IGLPADTEEKCIFTAKKFVELKPDCVRVYPTLVVKDTGLEKLMEQNKYNPFTLEESVN
                     IVKKVLVLFYVNNINVIRVGLQATDDIQIGKAVLAGPYHPAFRELVEADMIKDYLEFV
                     ILQNKNIKQMLVKSNKKNISKIIGNKKTNVKYMEEKFGVLLKTQESDLDINQLEIVLD
                     GKSLISANMRDIHRKLYDIYDL"
     gene            3322..6876
                     /gene="smc"
                     /locus_tag="KPD58_001885"
     CDS             3322..6876
                     /gene="smc"
                     /locus_tag="KPD58_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HBG0265654.1"
                     /translation="MYLKRLELKGFKSFPVKTDIIFKEGITAIVGPNGSGKSNISDAV
                     RWVLGEQSIKSLRGDKLEDVIFAGTDTKKPMNYCEVALTIDNSENQLELDFTEVTIRR
                     RAYRNGESEFFLNNKSCRLKDIKEVFLDTGIGKDGYSIIEQGKVDEILSNNPLSRRKV
                     FDEACGISKYRYKKQEAERNLSNTKENLERIDDVYIEIENQLKPLFNQQTKAKKYLEI
                     SEKLKTLEVNSFIREIEGIEKELSEVNEHRKVIEKELNEKEEQKNVVEKKQEDINKEV
                     EVLQDVIEKSVDYINSIKGVISKKESQINLIKERIRNFTNEISRKNLEIKDIKEKLNE
                     NKQYIKELESNKLSGSEELSTLQENIKVLEGSKDKQKIKLESLNNEIELLKESIIDIL
                     NKKQEFSNKLSTLNANKENMNIRDENINSEITELNKNIEIKSSELDTINKEFNMQNEN
                     LKNVNNRHKELSINLQDSISEHNKLEDEIQKSKYNLNGYNSKLNVYIDMENHYEGFNR
                     GVKEVLKNKNLKGVHGALGQIINVPEKYEKSIEAALGAYMQNIITDNEFSAKFAINYL
                     KQNNLGRVTFLPLNIIKSNKISLGNLKANTKFIGIASDLITFDEKYRNIIENILGRTI
                     LINNIDEGIKFAKETGHRFKIVTLDGEILNPGGSLTGGSLKTNGNILSRKRYINEYTE
                     KISNIKNEISHLELKRESLDKDVKNIKNEIDSHESKIKDLEKSIIIKSTSIKNVESEI
                     ESLKGSITKLENEKNDLNSNLNYTLEKSDDVRKDMEELDDLYNKNKEKIDALNEEIKR
                     YNDLYDKEKSEFDELNLSLVKKTEVYNSIVRDIKRISGENCELEEKNKQLEESLNYEE
                     HEIIKLQDSILTEEKEKENLTKQLGDSNRNLETRKIAKDDLKNSFDEINKELKTIDRQ
                     HIELKESLFKVGGRLERLKTSQDTYINKLFEQYDMTLVQALEIKDEDLDIDRKFLESL
                     KREIRSLGNINIDSIKEYEEIKERYDFYSEQKQDLEESMEEIEKLIHTLEENMKSEFE
                     IKFEEISKNFKYVYKRLFGGGCGELTILDKENLLESDILITAQPPGKKMKNLNLLSGG
                     EKALTAISILFAILITKPTPFCILDEIEAPLDDANIFRFGEFLKDLSKETQFISVTHR
                     RGTMEAADYIYGVTMQEKAISKVISLKLKEAQEITDII"
     gene            6903..8183
                     /gene="ftsY"
                     /locus_tag="KPD58_001886"
     CDS             6903..8183
                     /gene="ftsY"
                     /locus_tag="KPD58_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBG0265655.1"
                     /translation="MLKKLFGFGKDKEKEIEKKDAEEEIEVEDSVDNLENLEETIFSG
                     LEEEVIDKVEDIEEKNEESVNNEGTEEIENFEKIEVEMDSNGKEVENISNNDSQEEII
                     EELENYDEIESKEIEDKEDKKVNLFERLKQGLTKAKQGITDRIDEVLKSYTKIDEELL
                     EDLEEILITADVGVNTTMDIIERLRDKIKQKGITEPIKVREELKSIVEDILTNENSTL
                     DIEPAPCIILMVGVNGVGKTTTIGKLANRYKKDGKKVLLAAADTFRAAATEQLEIWAN
                     RTNVDIIKHQEGADPGAVVFDAIKAAKARKTDVLICDTAGRLHNKANLMNELGKVFKI
                     VDREFPEAKREVLLVVDATTGQNAVVQAKTFKEVADITGIVLTKLDGTAKGGVVLAVK
                     SEVDVPVKLIGVGESVEDLQDFNAKSFSDALFGN"
     gene            8286..8654
                     /locus_tag="KPD58_001887"
     CDS             8286..8654
                     /locus_tag="KPD58_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HBG0265656.1"
                     /translation="MNIEKMVEIGLLFEQYKELLTDKQKEIVALYYEEDYSLGEISEN
                     LNVSRQGVYDTLKRSEKILRDYEEKLHLVSKIQEQEKNIKIIKDKIIDIKEDLLHNRD
                     CANLIPKLENIEDVCREMIK"
     gene            8651..10003
                     /gene="ffh"
                     /locus_tag="KPD58_001888"
     CDS             8651..10003
                     /gene="ffh"
                     /locus_tag="KPD58_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBG0265657.1"
                     /translation="MIFEGLSDKLQGAFSKLKSKGKLTEVDVKNAMREVKLALLEADV
                     NFKVVKDFIKKVQERCVGQEVMQSLTPAQHVIKIVNEELTSLMGDVQSKVMISSKPPT
                     ILMMVGLQGAGKTTTAGKLGGYFKKQGKKPLLVACDIYRPAAIKQLQVVGEKLDIPVF
                     NMGDKENPINIAKAGLSHAIKNANDLVIIDTAGRLHIDEVLMDELKSIKSEVKPHEIL
                     LVVDSMTGQDAVNVAESFNEALGVDGVVLTKLDGDTRGGAALSIRAVTQKPIKFMGMG
                     EKLDDIEPFHPDRMASRILGMGDVLSLIEKAQENIDLEKAKELEQKIKKQELDFEDFL
                     QQMEQIQNMGPLDKILGMIPGMGNVKDQLGDVDLNGKEMKRTKAIIQSMTTYERRNPG
                     VLNASRKKRIAKGSGTSVQDVNRLIKQLNEMKKMMKMFAGSQKSMKKRGGLGGLPFFK
                     "
     gene            10041..10313
                     /gene="rpsP"
                     /locus_tag="KPD58_001889"
     CDS             10041..10313
                     /gene="rpsP"
                     /locus_tag="KPD58_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBG0265658.1"
                     /translation="MAVKIRLKRMGANKKPFYRIVVADSRAPRDGKFIEEIGYYNPIS
                     EPKQVRINDEKAIKWLATGAQPTEVVKKLLVKNGVIEKFEASKQAK"
     gene            10378..10605
                     /locus_tag="KPD58_001890"
     CDS             10378..10605
                     /locus_tag="KPD58_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HBG0265659.1"
                     /translation="MKELVVDIAKALVDNPDSVVVEEFEDNDGIVLKLTVAQEDMGKV
                     IGKQGRIAKAIRTVVRSVANRENIKVSLEIV"
     gene            10713..11228
                     /gene="rimM"
                     /locus_tag="KPD58_001891"
     CDS             10713..11228
                     /gene="rimM"
                     /locus_tag="KPD58_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HBG0265660.1"
                     /translation="MKEKLTHFKVGQIVNTQGLKGEVRVYPLTDDIERFDELKDFYLG
                     KDLNNKLAVEKVRYKGSMVIMKIKGIDSIEKAEKLKNKFMYVAREDSRELEEGEFFIA
                     DMIGMEVLTVDGKYVGILEDVLQYSANDVYVVKGEEDKEFMIPAIKKFVPTIDIDERK
                     MIIDPIKGMID"
     gene            11237..11932
                     /gene="trmD"
                     /locus_tag="KPD58_001892"
     CDS             11237..11932
                     /gene="trmD"
                     /locus_tag="KPD58_001892"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBG0265661.1"
                     /translation="MRFHIMTLFPEIFNSYMDESIMKRAVEKGIIEVHIYNIRDFSNN
                     KHKKVDDYPFGGGAGMVMTPQPIYDTYKHIITTHNINNPSVIYLTPKGKVYNQSMAKQ
                     MSLKEDIILLCGHYEGIDERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGV
                     LNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITK
                     ERRFDLYKKSNEK"
     gene            12117..12467
                     /gene="rplS"
                     /locus_tag="KPD58_001893"
     CDS             12117..12467
                     /gene="rplS"
                     /locus_tag="KPD58_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBG0265662.1"
                     /translation="MNEMLRAIEQEQLKNEVPNFGPGDTVKVHVRIIEGKRERIQVFE
                     GVVLKRQGGGARETFTVRKMSFNVGVERTFPVHSPKIEKIEVTRKGKVRRAKLNYLRG
                     RVGKAAKIKEARNK"
     gene            12788..13684
                     /gene="ylqF"
                     /locus_tag="KPD58_001894"
     CDS             12788..13684
                     /gene="ylqF"
                     /locus_tag="KPD58_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HBG0265663.1"
                     /translation="MSNEYEEYLMNDNLHINWYPGHMKKTKELVKNNLKLIDVVIELL
                     DARIPFSSKNPDIDRLVGDKPRVVVLNKSDMADRDKLNQWIEYYKKINIKAIPVDTIK
                     GVGINKIIEECKNVTREKMSSLKDKGRKERAIRIMIVGVPNVGKSSLINKLTGRKSTQ
                     TGDKPGVTKGKQWVRLKGNLELLDTPGILWPKFEDQEVALNLAFSRAIKDEILDTETL
                     ALRLIEKLMKIEPEKLKARYKLDCLGETPIETMDMIGHKRGFITGKKELDYTRIATTV
                     LNEFRDGKIGNITLEVPENVKR"
     gene            14073..15365
                     /gene="brnQ"
                     /locus_tag="KPD58_001895"
     CDS             14073..15365
                     /gene="brnQ"
                     /locus_tag="KPD58_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBG0265664.1"
                     /translation="MGKTKDVIVFGFALFAMFFGAGNLIFPPYLGIITGPEWLIAFLG
                     FTFADAGLALLAVMATAKFDGNVVEMFKRCGMKLGILIGCADILCIGPFLAIPRTGAT
                     TYEMGIMPLFGTSIPVLLFCILFFAISYVLTIRPSKVVDIVGQFLTPALLIALAFIII
                     KGIISPLGDIVDKPMIPNVFAEGIGQGYQTMDAFAAIALASVLIVSLNDKGYSTISDK
                     LKMIGKAGVLACGGLALVYGGLCFLGATVSTMYGTDAVQSQVIVNITEGLLGNVGKAI
                     LAVVVSLACLTTSIGLTSATGQYFSRLTKGKLSYEKIVLAVSVFSAVVASFGVGTIIK
                     IASPILSIVYPPSIVLIILAFFNEKIKNDNVYKGAVYMSLLVSILTVISSYGVAVPVV
                     NSLPLNSLGFNWVVPVIIAGIIGNFIPSKSQSNTLGTN"
BASE COUNT         6536 a         1476 c         2944 g         4473 t
ORIGIN      
        1 taataataaa aatatagaat aagtaatttg ccaaatactt gtttatattg tataacttat
       61 gatattatga tattagtaaa aatttgtaat cctgacttga cagaattcaa gtaagagata
      121 taattttaaa ggcatagtaa tgtaaaattt actaaaaaat aagaaattta ttttaatatg
      181 gaggaacaaa aaatggtatc agcaggtgat tttagaaaag gtgttacatt tgaaaaagat
      241 ggacaaccat gtttagtagt tgattttcaa cacgttaagc caggtaaagg agctgctttc
      301 gttagaacta aatacaaaaa cttaaaaaca ggagctataa gagaagaaag tttcaatcca
      361 agtgaaaaat tccctaaagc tgttatagat acaagacaaa tgcaatatct atacaatgat
      421 ggtgagttat attattttat ggatcaagaa aattttgaac aaataccatt aaactatgag
      481 caagttgaag atgcaattaa gttcttaaaa gaaaatgaag ttgctacaat aagattttac
      541 caaggacaac cattccaagt ggaagcgcca aactttgcag agctagaagt tacagataca
      601 gagccaggta taaaaggtga tacagcaagt aatgtaacta aagcagctac agttgaaact
      661 ggagcagttg ttcaagttcc attatttata aatactggag ataaagtaaa gatagatact
      721 agaacaggtg aatatttatc aagagtataa aaatttatgt atataaaagc ttatatgtct
      781 taataatata agatatatga gcttttagtt tatatataaa aaagaaagcg aggggtgtta
      841 catgaaacat ttatatgaag aagttgcata cttaaaaggc ctagcagaag gactagaaat
      901 aagtgctgaa agtaaagaag ggaaaatgat acataaaata gttgatgcat tagaagtatt
      961 tgcagatgct attgtaacat tggatgaaga acaagaagaa cttcaagatt ttgttgaatc
     1021 tatagatgaa gatttagcag atttagaaga agatattgct gacatggaag aagatcttta
     1081 tgaagaagat gacgatgagg atgatgagga cttcagctat atagaaatgg aatgcccaaa
     1141 ttgtggagag ttagtagaaa tagacgaaga tttgttatat gatgatgaag tagatgttgt
     1201 ttgtcctgat tgtaaggctg tgatattatc atcagaagat gattgtgatg atgattgtac
     1261 atgtggtggt tgttcaagtt gtgatgatag agagtaaaaa ttcataagaa ttaaaactgc
     1321 ctatgatgcc tatgagatat tttgcttcat aggtagtttt atttttatga ggaagggtat
     1381 tatgtatttt ggttttgcaa tacttggaca aaaatagtac actagaattt atagaatagg
     1441 attttggagg gatgtaaata tgaaaataag taaaaaatta ctagataata tacaaagatt
     1501 tgaaaatgtt ataaattata aatttaaaaa taaagaatat atactagaag ctcttactca
     1561 tagttcatat tctaatgaaa ataaaaaata taattttaac gaaagattgg aatttttagg
     1621 ggattcggtt ttaggcatag tgataagtga ttatttattt aatgaagaag ctaatctgcc
     1681 cgaaggggaa ctaactaaac ttagagcaaa tatagtttgt gaagattcat taagtgaagt
     1741 agctaatgat ataaatttag gtattcacat gttattaggt agaggagaag aagcaacagg
     1801 aggaagacat aggacttcaa tattagcaga cgcatttgaa gctgtaatag ctgctatata
     1861 tcttgatgga ggttttgaaa gtgcaagaca gtttatactt catcatatgg aaaatataat
     1921 ttatgattca agaaaaggaa atatatttag agactacaaa acgcatcttc aagaggtgtt
     1981 acaagggaat ggagaaaata atatctggta caggcttata gaagaaaaag gaccagacca
     2041 taataaaaga tttgtaatgg aagttggaat taatgacgat gtacttggta taggagaagg
     2101 caagagtaaa aaagaagctg aacagttagc tgctaaaata gctttaaaga aaaaattatg
     2161 ggaaaaataa atataatatg taaaaatata aaaagaaata tttatattag aaggatgttg
     2221 tagatgaaaa agagaattat acctatattt gttcctcaca aaggatgtcc tcatgattgc
     2281 atattttgta atcaaaaaaa aataacaggt gtgagtactg atgttactag tgaagatgca
     2341 agaaatataa ttgaggaatg tttagagact atagataaag atgcagatgt agaaatagca
     2401 ttttttggtg gtagctttac tgctatagat attgatatac aaaaaagttt attatcagtt
     2461 gctaaagaat atgtagaaaa aggtcttgta aaagatatta gaatgtctac aagaccagat
     2521 tgtattgatg aagatatatt aagtatgtta aaagaatata agacaagtat aattgaacta
     2581 ggagtacaat ccttagatga aaaagtatta ttagatagta taagaggtca tcaatctgaa
     2641 attgtataca agagttcaaa gatgataaaa aatagtggaa tcaaacttgg attacagatg
     2701 atgattggtc ttccagctga cacagaagaa aagtgtatat ttacagcaaa aaaatttgta
     2761 gagctaaaac cagattgtgt tagagtctat ccaactttgg tagtaaaaga tacaggtctt
     2821 gaaaagttaa tggaacaaaa taaatataat ccatttactc ttgaagagag tgtaaatata
     2881 gtaaaaaaag ttcttgtact gttttatgtt aataatatca atgtaataag agttgggctt
     2941 caagctactg atgatataca aattggaaaa gctgttttag caggaccata tcatcctgca
     3001 tttagagagt tggtagaagc agatatgata aaagattatt tagaatttgt tattttgcaa
     3061 aataaaaata taaagcaaat gttggtcaaa tctaataaaa agaatatttc taaaatcata
     3121 ggaaataaaa aaacaaatgt taaatatatg gaagaaaaat ttggtgtgct tttaaaaaca
     3181 caagaatcag atttagatat aaatcaatta gaaattgtgt tagatgggaa aagtttaatt
     3241 agtgctaata tgagagacat tcatagaaaa ttgtatgaca tttatgacct ttagttttaa
     3301 taatgaaggg agagaaaaga tttgtattta aaaagactag agttaaaggg atttaaatcc
     3361 tttccagtaa aaacagatat tatttttaaa gaaggaataa cagccatagt aggaccaaat
     3421 gggagtggaa aaagtaatat atctgatgct gtaagatggg tattaggaga acagagtatt
     3481 aagagtctta gaggtgataa actcgaagat gtaatatttg caggtacaga tacaaaaaaa
     3541 cctatgaatt attgtgaagt ggctcttact attgataatt ctgaaaatca attggaatta
     3601 gattttacag aagtaactat aagaagaagg gcttatagaa atggtgaaag tgaatttttt
     3661 ctaaataata aaagttgtag attaaaagat ataaaagaag tctttttaga tactggtatt
     3721 ggaaaagatg gttattcaat catagaacaa ggaaaagtag atgaaatatt aagtaataat
     3781 cctttaagca gaagaaaagt ttttgatgaa gcttgtggca tatcaaaata tagatataaa
     3841 aaacaggaag cagaaaggaa tttaagtaat acaaaggaaa atttagaaag aatagatgat
     3901 gtatatatag aaattgaaaa tcaattaaag cctcttttta atcaacagac aaaggcaaaa
     3961 aagtacttgg aaataagtga aaaattaaag acacttgaag taaatagctt tataagagaa
     4021 attgaaggaa tagaaaaaga attaagtgag gttaatgaac atcgtaaggt tattgaaaag
     4081 gagctcaatg aaaaagaaga acaaaaaaat gttgtagaga aaaagcaaga agatattaat
     4141 aaagaggtag aggtattaca agatgtaatt gaaaaatctg tagattatat aaattcaatt
     4201 aaaggagtta tatcaaaaaa agagtcccaa ataaatttaa ttaaggaaag aataagaaat
     4261 tttacgaatg aaattagtag aaaaaattta gaaataaagg atataaaaga gaagctaaat
     4321 gaaaataagc aatacataaa agaattagaa agcaataaat tatcaggtag tgaagaacta
     4381 tctaccttgc aggaaaatat aaaagtattg gaaggaagta aagataaaca aaaaattaag
     4441 ttagaatccc taaataatga gatagaatta ttgaaagaaa gtataattga tatattaaat
     4501 aagaaacaag agtttagtaa taagctgtct actttaaacg caaataaaga aaatatgaat
     4561 atcagagatg aaaatataaa ttcagagata acagagttaa acaaaaatat tgaaataaaa
     4621 tcatctgaat tagatactat aaataaagaa tttaatatgc aaaatgaaaa tttaaagaat
     4681 gtaaataata gacataaaga attatcaatt aatttgcagg attcaattag tgaacacaat
     4741 aaactagaag atgaaataca gaaaagtaaa tataatttaa atggatataa ttcaaagtta
     4801 aatgtctata tagatatgga aaatcattat gaaggattta atagaggagt aaaagaagta
     4861 ctaaaaaata aaaatttaaa aggagttcat ggagcattag gacagattat aaatgtacct
     4921 gaaaagtatg agaaatctat agaagcagct cttggtgctt atatgcaaaa tataataact
     4981 gataatgagt ttagtgctaa gtttgcaata aactatctaa aacagaataa tttaggtaga
     5041 gtaacatttc ttccattaaa tataattaaa tcaaataaaa taagtctagg aaatttaaaa
     5101 gcaaatacta agttcatagg aatagcaagt gatttaatta catttgatga aaagtataga
     5161 aatataattg aaaatatact tggaagaaca atacttataa ataatataga tgagggcata
     5221 aaatttgcaa aagaaactgg tcataggttt aaaatagtaa ctttagatgg tgaaatctta
     5281 aatccaggag gttctttgac tgggggaagt ctaaaaacta atgggaatat actatcgaga
     5341 aaaagataca ttaatgaata tacagaaaag ataagtaata tcaagaatga aatttcacat
     5401 ctagaattaa aaagagaatc tttagacaaa gatgtgaaaa atatcaaaaa tgaaattgat
     5461 tcacatgaaa gtaaaataaa agatttagaa aaaagtataa taataaaaag tacaagtata
     5521 aagaatgttg agtctgaaat tgaatcgtta aaaggtagta ttactaaatt agaaaatgag
     5581 aagaatgatt taaattctaa cctaaattat acacttgaaa aaagtgatga tgttagaaaa
     5641 gatatggaag agttagatga tttatacaat aaaaataaag aaaaaattga tgcattgaat
     5701 gaggaaataa aaagatacaa tgatttatat gataaagaaa aatctgaatt tgatgaattg
     5761 aacttaagtt tagtgaagaa aacagaagtt tacaatagta tagttagaga tataaaaaga
     5821 atatctggtg aaaattgtga gctagaagaa aaaaataaac aattagaaga gtcattaaat
     5881 tatgaagaac atgaaataat taaattacaa gattctatac tcactgaaga aaaagaaaaa
     5941 gaaaatttaa caaaacaact aggggatagt aatagaaatc ttgaaactag aaaaatagca
     6001 aaagatgatt taaaaaatag ttttgatgaa attaataaag aattaaaaac tatagataga
     6061 cagcatatag aacttaaaga aagtttattt aaagttggtg gaagattaga aagattaaaa
     6121 actagtcaag acacatatat aaataagttg tttgagcaat atgatatgac cttagtacaa
     6181 gcattggaaa ttaaagatga agatttggat atagatagaa aattcttaga aagtctaaag
     6241 agagaaataa gaagtcttgg aaatataaat atagactcta taaaggaata tgaggaaata
     6301 aaagaaagat acgattttta tagcgaacaa aagcaagatt tagaagagtc tatggaagag
     6361 atagaaaaac ttatacatac tttagaagaa aatatgaaat ctgaatttga aattaagttt
     6421 gaagaaataa gtaaaaattt taaatatgta tataagagat tatttggtgg aggttgtgga
     6481 gaattaacta ttttagataa agaaaattta ttagaaagtg atatactaat aacagctcaa
     6541 cccccaggta aaaaaatgaa aaatttaaac ctattatctg gtggagagaa agctttaacg
     6601 gcaataagta tattatttgc tatattgatt acaaagccaa caccattttg tatattagat
     6661 gagattgaag cgccacttga tgacgcaaat atatttagat ttggagaatt tttgaaggat
     6721 ttatctaaag agactcaatt tatatcagta actcatagga gaggaacaat ggaagctgct
     6781 gattatatct acggagttac aatgcaagaa aaagccattt caaaagttat tagtttaaaa
     6841 ttaaaagaag ctcaggaaat aacagatatt atataactta gattgacata ggaggaataa
     6901 cagtgttaaa aaaattattt ggttttggaa aagacaagga aaaggaaata gaaaaaaaag
     6961 atgctgaaga agaaatagaa gttgaagaca gtgttgataa tttagaaaac ttggaagaaa
     7021 ctattttttc aggacttgaa gaagaagtga tagataaagt ggaagatatt gaagaaaaaa
     7081 atgaggaaag tgtcaataat gaaggcactg aagaaatcga aaactttgaa aaaatagaag
     7141 ttgagatgga tagtaatgga aaagaagtag aaaatatttc aaataatgat tctcaagaag
     7201 agataatcga agaattagaa aattatgatg aaattgagtc taaagagata gaagataaag
     7261 aagataagaa agttaacttg tttgaaagac taaaacaagg attgacaaaa gcaaagcaag
     7321 gaataactga tagaatagat gaagttttaa aatcatatac aaaaatagat gaagaattac
     7381 ttgaagattt agaggaaatt ttaataacag cagatgttgg tgtaaataca actatggata
     7441 taattgaaag actaagagat aaaataaagc aaaaaggtat tactgaacca ataaaggtaa
     7501 gagaagaact taaatctata gttgaagata ttcttacaaa tgaaaactct acactagata
     7561 tagaaccagc accatgtata atattaatgg tgggtgtaaa tggggtcggt aagactacta
     7621 caataggtaa actagcaaat agatacaaaa aagatggtaa aaaagtttta ttagcagcag
     7681 cagatacatt tagagcagca gcgacagagc aattagaaat ttgggcaaat agaaccaatg
     7741 tagacataat aaaacatcaa gagggagcag accctggagc tgtagtattt gatgctataa
     7801 aagctgcaaa agctagaaaa acagatgtat taatatgtga tacagcagga agattacaca
     7861 ataaggctaa tcttatgaat gaacttggaa aagtatttaa aatagtagat agagaatttc
     7921 cagaagcaaa acgtgaagta cttcttgtgg tggatgctac aacaggtcaa aatgcagttg
     7981 ttcaagcaaa gacatttaaa gaagttgcag atataacagg tatagtactt acaaaacttg
     8041 atggaactgc aaaaggtgga gtagtccttg cagtaaaatc agaagtagat gtacctgtaa
     8101 aacttattgg agttggagaa agtgtagaag acctacaaga tttcaatgca aaatcatttt
     8161 cagatgcatt atttggtaac taatgttctt ttaaaaataa tggttgacaa gagtgttgtt
     8221 atgatataaa atacaatagt gtaaagtaaa taacctttac agtatactgc taggaagtgt
     8281 aagatatgaa tatagaaaaa atggtcgaaa ttggattgtt atttgaacag tataaagaat
     8341 tattaactga caagcagaaa gaaatagttg ctctatacta tgaagaagat tattctttag
     8401 gtgaaattag tgaaaatctg aatgtatcaa gacaaggtgt ttatgatacc ttaaagcgtt
     8461 cagaaaaaat actaagagat tatgaagaaa agttacactt agtatcaaag attcaagagc
     8521 aggaaaaaaa tattaagatt ataaaagata aaattattga cattaaagaa gatttattgc
     8581 acaatagaga ttgtgctaat ttaatcccta agctagaaaa tatagaagat gtatgtaggg
     8641 agatgataaa atgatatttg aaggattgtc tgataaacta caaggggcat ttagtaagtt
     8701 aaaatctaaa ggaaaactta cagaagtaga tgtaaaaaat gctatgagag aagttaaact
     8761 tgctcttctt gaggcagatg ttaactttaa agtagttaaa gatttcataa aaaaagttca
     8821 agagagatgt gttgggcaag aagttatgca aagcttaact ccagctcagc atgtaatcaa
     8881 aattgtaaat gaagagctta caagtttaat gggagatgta caaagtaaag taatgatttc
     8941 ctcaaagcct cctacaattt taatgatggt tggattgcaa ggtgcaggta agactactac
     9001 agcaggaaaa cttggaggat attttaaaaa gcaaggtaaa aaacctctat tagtagcttg
     9061 tgatatttat agaccagcag ctataaagca acttcaagtt gttggagaaa aattggatat
     9121 acctgtattc aatatgggag ataaagaaaa tccaataaat attgcgaaag caggtttaag
     9181 ccatgcgatt aaaaatgcta atgatttagt tattatagat acagctggta gacttcatat
     9241 agatgaagta cttatggatg agttaaagtc tattaaatca gaagttaagc cacatgagat
     9301 acttttggtt gtagattcta tgacaggtca agatgctgta aatgttgcag aaagcttcaa
     9361 tgaggcactt ggtgttgatg gagtagtatt aactaaattg gatggagata ctagaggtgg
     9421 tgctgcactt tcaataagag cagttacaca aaagcctata aaatttatgg ggatgggaga
     9481 aaagctagat gatatagaac ctttccaccc agatagaatg gcatcaagaa tattaggtat
     9541 gggagatgtc ttaagtttaa tagaaaaagc acaagaaaat atagacttag agaaagctaa
     9601 agaattagag caaaaaatta aaaaacaaga gcttgatttt gaagatttct tacaacaaat
     9661 ggaacaaata caaaatatgg gacctcttga caagatactt ggtatgatac ctggtatggg
     9721 aaatgtaaaa gaccaacttg gagatgtaga tttgaatggc aaagaaatga agagaacaaa
     9781 agccataatt cagtcaatga ctacatatga gagaagaaac ccaggagtat taaatgcttc
     9841 aagaaagaaa agaatagcta aaggcagtgg aactagtgta caagatgtaa atagacttat
     9901 aaaacaactt aatgaaatga aaaaaatgat gaagatgttt gctggttcac aaaagagtat
     9961 gaagaaaagg ggcggcttag gcggtttacc tttttttaaa taaaatataa aaaagtaaaa
    10021 attattcgga ggtgactgta atggcagtta aaataagatt aaaaagaatg ggagctaaca
    10081 aaaaaccttt ctacagaata gtagtagctg attcaagagc tccaagagat ggaaaattta
    10141 tagaagaaat aggatactat aatccaattt ctgaacctaa acaagtaaga ataaatgatg
    10201 aaaaagctat aaaatggtta gctactggtg ctcagccaac agaagtagtt aaaaaattac
    10261 ttgtaaaaaa cggagtaata gaaaaattcg aagcttctaa acaagcaaaa taattttttc
    10321 ttggcaatta gtttaagtaa gggctcattg ctaaggttac tttaggagat gaaatatatg
    10381 aaagagctag ttgtagatat agctaaggct ctagttgata atcctgactc agttgttgtt
    10441 gaggaatttg aagataatga tggtatcgtc ttaaagctta cagttgctca agaggatatg
    10501 ggtaaggtta ttgggaagca gggaagaata gctaaagcta taagaactgt cgtaagatct
    10561 gttgcaaata gagaaaatat aaaagtttct cttgagatag tataaaatag gaggattagg
    10621 tataatgcct agtccttttt atataaatgc gtagtttaag attttatgat gatatgacca
    10681 cataagatta aattttttaa gaaggtgata acatgaaaga aaaattaact cattttaagg
    10741 taggtcaaat agtaaataca caaggtttaa aaggtgaagt aagagtttat ccgcttactg
    10801 atgatataga gaggtttgat gaattaaaag atttctatct gggaaaagat ttaaataata
    10861 aattggctgt tgaaaaagta agatacaaag gttctatggt tataatgaag ataaaaggca
    10921 tagacagcat agagaaagca gaaaaactaa aaaataaatt tatgtatgta gctagagaag
    10981 atagcagaga gcttgaagaa ggcgaatttt ttatagcaga tatgattgga atggaagttt
    11041 taactgtaga tggaaaatat gtaggaatac tagaagatgt tcttcaatac tcagcaaatg
    11101 atgtctatgt agtaaaaggt gaggaagata aagaatttat gataccagct ataaaaaaat
    11161 ttgtacctac aatagatatt gatgaaagaa aaatgattat agaccctatt aagggaatga
    11221 tagattaggt ggtaatatga gatttcatat aatgacactt tttccagaaa tatttaattc
    11281 gtacatggat gaaagtataa tgaagagagc agttgaaaaa ggtataattg aggttcatat
    11341 atataatata agggattttt ctaataataa acataaaaaa gtagatgatt atccatttgg
    11401 aggaggagcg gggatggtaa tgactcctca acctatatat gacacatata agcatataat
    11461 aacaactcat aatataaata acccaagtgt aatttatctt actccaaaag gtaaggtata
    11521 caatcaatct atggcaaagc agatgtcttt aaaagaagat ataatactac tatgtggtca
    11581 ttatgaagga atagatgaga gaatcattga tttaatagtt actgatgaaa tttcaattgg
    11641 agattatgta ttaactgggg gagagctccc agcacttata atgatagatt ctatatcgag
    11701 attaatacca ggtgtattaa accaagagga atcatttgaa gaagaatcat ttaaagataa
    11761 cttattggaa tatcctcatt acactagacc tagagatttt gaaggattaa aagtaccaga
    11821 agtgctttta tcagggaatc ataagaagat agatgagtgg agaagagaag aatctataag
    11881 gataactaaa gaaagacgat ttgatttata taaaaagtct aatgaaaaat aaaaatataa
    11941 tgaaaaatta atatatatgt atacaataaa gtacctataa atggaattaa aaacataaaa
    12001 gtgcttgtaa taactattga agtatagtat aatataaaat gttgaaaata cgggcgttcc
    12061 tctgtttaca ctggtcgggt agttaagaac gtctatgata tctaggagga agaaagatga
    12121 atgaaatgct aagagcaatt gagcaagaac aattaaaaaa tgaagttcct aattttggac
    12181 ctggggacac agttaaagta cacgttagaa ttatagaagg aaaaagagaa agaatacaag
    12241 tattcgaagg tgtagtatta aagagacaag gtggaggagc aagagaaact ttcacagtta
    12301 gaaaaatgtc tttcaatgtt ggtgtagaaa gaactttccc agttcactct ccaaaaatag
    12361 aaaaaataga agttactaga aaaggtaaag taagaagagc taaactaaac tacttaagag
    12421 gtagagtagg taaagctgct aagataaaag aagctagaaa taaataatta aaaactttac
    12481 ttataaatgg ggggctgatt tatcagtcct ctttctattt taatgaaaat tacagcaaat
    12541 aattatacta gaaataaata gaaatatttg ttgaaaatat acattattta tctatgaatt
    12601 tatagccttt aaaatgaaaa ataaaattgg taaatattgc attatatttt gtaacaatat
    12661 taaaaataag agaacagtat tatgtaaagt atgacgttaa aaagatgaca atattatagt
    12721 ttgttaagat agatttttta agtggaatta ggatataata atataatatt aataaggagt
    12781 gatacatgtg tcaaatgaat acgaagagta tcttatgaat gacaatctac atataaactg
    12841 gtatccaggt catatgaaaa aaactaagga gttagtaaaa aataacttaa aattgattga
    12901 tgtagtaatt gaacttttgg atgctagaat cccgtttagt agtaaaaatc cagacataga
    12961 caggcttgta ggagataagc caagagttgt cgtacttaat aagagtgata tggcagatag
    13021 ggataaattg aatcaatgga ttgaatacta taagaaaatt aatatcaaag ctataccagt
    13081 agatacaata aaaggcgttg gtataaataa gataatcgaa gaatgtaaaa atgtaactag
    13141 agaaaaaatg agttctttaa aagacaaagg gagaaaagaa agagctatac gaataatgat
    13201 agttggagtt ccaaacgttg gtaaatcgtc tcttataaac aaattaacag gtagaaaaag
    13261 cactcaaact ggagataagc caggagtaac gaaagggaaa caatgggtta gattaaaggg
    13321 aaatcttgaa cttttagata cacctggaat actttggcct aagtttgaag accaagaagt
    13381 tgcattaaat ttagcattta gtagagctat aaaggatgag attttagata ctgaaacttt
    13441 ggctttaaga cttatagaga aacttatgaa aatagaacct gagaagttga aagccagata
    13501 taagttagat tgtttaggag aaactccaat tgaaactatg gatatgattg gccataaaag
    13561 agggtttata acaggtaaaa aagaactaga ttatacacgc atagccacaa ctgtattaaa
    13621 tgaatttaga gatggaaaaa taggaaatat aacattggaa gttccagaaa acgttaaaag
    13681 atagatttta aaacaaaatc aaaaatttaa tgttaaaaat ttaaaaaagc cttttagcaa
    13741 aggctttttt atttttttga aatatagaca aatatctcca tataaaagta taaattaggt
    13801 tagaaaacct ttaaaaatat taatttaaga caaatatacc taatattttg aaaattaaaa
    13861 tgagtgtgat tatttaaaat tttagcatat tgacttgctt gtttcgtata atatataata
    13921 agcacaaata aatacaatat attaaaaatt taatttattt aattttatta atttaataat
    13981 aagttcttat tgtttaattc aacttactta tgcaagcgta aacaatcgag acattttatt
    14041 aatttaataa ggttaaaaag ggggatttta taatgggtaa gaccaaggat gttatagtat
    14101 ttggatttgc gttatttgca atgttctttg gagcaggtaa cttaatattt ccaccttact
    14161 tgggaataat tacaggtcca gaatggttaa ttgccttctt aggatttacg tttgctgatg
    14221 ctggattggc acttctagca gtaatggcta cagctaaatt cgatggaaac gtagttgaaa
    14281 tgtttaaaag atgtggtatg aagttaggga ttcttatagg ttgtgcagat attttatgta
    14341 taggcccatt cttagctata ccaagaacag gtgcaacaac ttatgaaatg ggtataatgc
    14401 ctttgtttgg aacttcaatt ccagtgttat tattctgtat actttttttt gcgatttcat
    14461 atgtactaac aataagacct tctaaggtcg tagatatagt tggacagttt ttaactccag
    14521 ctcttctaat agctttagca tttataatta taaaaggtat tatatcacct cttggagata
    14581 tagtagataa acctatgata cctaatgtat ttgcagaagg tataggtcaa ggatatcaga
    14641 caatggatgc ttttgcagca atagcacttg catcagtgct aatagtatct ttaaatgata
    14701 aaggatattc tactattagt gacaagttga aaatgattgg taaggcgggt gttcttgctt
    14761 gtggaggatt agctctagtt tatggaggat tatgtttcct tggggcaact gtatcaacaa
    14821 tgtatggaac agatgcagta caatcacaag taatagtaaa tataactgaa ggattgcttg
    14881 gaaatgttgg gaaagcgatt ttagcagtag tagtatcatt agcatgttta actacatcta
    14941 taggtcttac atctgctaca ggtcaatatt tctctagact tacaaaaggt aaattgagtt
    15001 atgaaaaaat agtattagct gtatctgtat tcagtgcagt agtagcatct tttggtgttg
    15061 gaactataat aaagatagca tctcctatac taagtatagt ttatccacca tcaattgttt
    15121 taataatttt agctttcttc aatgaaaaaa ttaagaatga taatgtatac aaaggagcag
    15181 tatatatgtc attgcttgta agtatactaa cagttatatc tagttatgga gtagctgttc
    15241 cagtagtaaa ttctttacct cttaattcat taggatttaa ctgggtggtg ccagttataa
    15301 tagctggaat aattggtaat tttataccat caaagagtca atctaataca cttggaacaa
    15361 actaagtttt gtttaattaa aatataatca attgtaaata aatgagttaa ctaaaagtaa
    15421 aaggagtta
//
LOCUS       DAELLV010000078        15214 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.078, whole genome shotgun sequence.
ACCESSION   DAELLV010000078 DAELLV010000000
VERSION     DAELLV010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15214
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.078"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            406..1443
                     /locus_tag="KPD58_001896"
     CDS             406..1443
                     /locus_tag="KPD58_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265665.1"
                     /translation="MLKKWFGIVKKRQKSESVKEEGEVILKNEKILSEEKLIDEEGVV
                     VNIDNQILTKVVNDDNEIEEKIIEEDLLISENTIKLDDKEAIINDKNIELCKKEVQVE
                     SEKIGLNKFEGLDQNENYNLEKNVIEEKKVSECLTEEDLEYIKEIKIKRGKSIKAINL
                     YTKEEWVFDTHIQCSKKLKVPLGYIRENLKYGYMDYFGDAINYLSEVLNIDEYCKSEW
                     SYLDNSKSPSEIFNILNNKIFSIRLSNEKRNEILTNDKIEALKMNYRFECIDEEYDEY
                     FKKYKSIIKRGGKKKVELVNKKGDILEIFKSLEECAIYLQKEKNEVIQMLKYGDTKVG
                     RNFIRYSLRSI"
     gene            1554..2696
                     /locus_tag="KPD58_001897"
     CDS             1554..2696
                     /locus_tag="KPD58_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG0265666.1"
                     /translation="MSEEKNPKENTLDESKNSDNVNSKKDDVSDEKNTDNKVKKREIK
                     LNNKTLKAIVAIVAVGAICLGGGYVFGKEAGRKLPATSKHYNSSEVVATIGDKKITGE
                     MLRKRMEPLFYLNAKTTMTDDEIRSYESNMIETMITSDILSSEAAKEKISITNDQLDA
                     QYNNLMSSIESAMGMTKEEFLKQFDLTEKYIKDDMKKELVASKYLNEKSKVTDKEAEN
                     YYNKNKSNYLQVRASHILIKTVDDKGKQVSSSKKAELKKEAEEILKKAQAGEDFATLA
                     KKYSEDSSAESGGDLGFFGKGQMVESFEKAAFALKKGEVSNKLVESDYGYHIIKKTDE
                     KYQPFEEVKSDLVSSLTSEKQNLLIQNLKEKYNVKITDKYQMKAKE"
     gene            3150..4541
                     /locus_tag="KPD58_001898"
     CDS             3150..4541
                     /locus_tag="KPD58_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBG0265667.1"
                     /translation="MSSTESVSKKSGHPKSFWLICMTILWERFSYYGLTALLVLFFTA
                     SVAQGGLGLSTQEATSMFGLFTGLIYLTPLVGGWLADKVLGQQKCISIGALLIAIGDF
                     VLFASSTSIAMVWVGLSIIILGNGFFKSSCSNIVGDIYPKTDVSRKDAAYSLFYMAVN
                     VGAFIAPIICGLLSDNVFAVRGASGDIVSYGYKMAFLICGIGMMLGMIIFTLLAPKLL
                     NEVGKYPVTKGNKGEKIEYGPLTKLEKNRIIAMVILFLFVVLFWTAYYQTQSSFMIYV
                     RDNVNRTLFGFEMPVAWLTSLNAILCVALCPVLATLWVKLSHTKRGDFSIPVKMGLGM
                     IIMGVGFIVMVFATLANGPDGMVKASILFIVLTYIFTTVGELFLSPIGLAMFNKLAPA
                     KYATLSMGAWYLTSFFASLLSGKIAGFTATLGFLQIFGGIATVLIIFGLILLGIRNVL
                     TRLMAMDLLTKEK"
     gene            4794..6587
                     /locus_tag="KPD58_001899"
     CDS             4794..6587
                     /locus_tag="KPD58_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG0265668.1"
                     /translation="MNIKDRLSGLRKFMEEKNIDAYMIPSSDNHQSEYVGDYFKSREF
                     ISGFNGSAGTVIVTKDEAGLWTDGRYFIQAESQLEGSTIKLFKMGQEGCPTTDEYLYK
                     NIPEGGTLGFDGRVISAREGATLAEKLSKKGIKIEYQYDLIDGIWPDRPALSDSKAFL
                     LDVKYCGESFSSKLARLREKMSEKGTSTHVITTLDDIAWLFNIRGGDVKYNPVVLSYA
                     VITLKEVYLFVDESKLNEEILNELAKENVQIKPYNDVYEFVKNIDKTEKVLLDGTKLS
                     YTIYNNIPCEVEKVDEFNPVMFFKAQKNEVELENIRNSHVKDGVAFTKFMYWLKKNVG
                     KMEITEISATQKLEDLRREQEGFFEPSFNTIAAYKEHAAMMHYSATPESNYKLEAEGL
                     FLVDSGGQYYDGTTDITRTTVLGPISDELKLHFTSVARGMINLSKAKFLHGCRGYNLD
                     ILSRSCMWNMGIDYQCGTGHGIGFVLNVHEAPNGFRWRVVPERFDSAVLEEGMVTTNE
                     PGIYIEGSHGIRTENEIVVRKAEKNFYGQFMEFEVVTLAPIDLDGIVPELMNKDEKDY
                     LNWYHKLVYDKISPFLTDEEREWLKVYTRAI"
     gene            complement(6668..8050)
                     /locus_tag="KPD58_001900"
     CDS             complement(6668..8050)
                     /locus_tag="KPD58_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBG0265669.1"
                     /translation="MSVLKTQEKLKQPRDLYFCCSIFTFVGLAYYGMKLVLLLFLAEQ
                     VSKGGLGLTPAESASMLASFLAWTYFSPVIGGWVSDRFLGPKNCIILGMLMVSIGYFL
                     GYIANSKMDINIMIFLSGLGIGFYKGNLHTMVGNLYTNDDPRKDGAFSITYMATNLGT
                     LFGPLICGLVANEWFAMKSGLSISMYGYRYVFLFSSITVLIGLFFFIFGNRKFYKPLN
                     NDPINGRESIRKSLEYKKMLASRPLTLIEKKRIIVILVLAFFTVFFWIAYNQASMSIA
                     LYTKEHINLTVGNFQIPTSWIDSYNGLLCVILGPISALVWVKLSQTKKGDLSVPKKMS
                     LGFILLAIAFLFMIVAVIQTGTDPNSIHKASVFWVVGFLTFQSIGEICFSPVGYGMVN
                     KLSPEKYISFLMGVWFLGKFAANKLSGYTQAIIDQLGMLQVFIVIPSFLLIFGIILLI
                     MNKKLVELSN"
     gene            8308..8655
                     /locus_tag="KPD58_001901"
     CDS             8308..8655
                     /locus_tag="KPD58_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBG0265670.1"
                     /translation="MIRKKSELQVEVKENLNGGIGKVKLENIIQNEELKGKGRLFKRV
                     ALPVGSSIGVHDHTTDFEVYYILKGKGKVFDNGEFVEVNEGDVVYTADGEKHSIENIG
                     EEDLEFVALVLYM"
     gene            8673..9707
                     /locus_tag="KPD58_001902"
     CDS             8673..9707
                     /locus_tag="KPD58_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265671.1"
                     /translation="MTGKIEEIKDKINRFCNSNLNQEYKDISFKILQNLLDNNKEIIH
                     SSRADIWSSAILNIVLEQNLLYNKKHPLHITKKEFSKQIGVSLNTINNKSSLIKDVCS
                     IDFLNQDTIAISNVEFWVKETNSKSKSVDLDLEKKKILYKRYIKLSQDANSDIESIRY
                     MEEAVNIGKSMITKEDKELGFWKGLSTRAYMIALESLARKLESINNLKESKKVLEYLI
                     EINPSDEQGIRYKLLNVLIRLNDRTSINKLFELYKEEKSATWMYSKALYYFKNKSMFL
                     ANDSIKVARSKNKYVGLYLLDWRNAFGREFVTEEEKAEAVYYYDENIVIWNEVKGSMD
                     WLLKKMLEFS"
     gene            complement(9756..10805)
                     /locus_tag="KPD58_001903"
     CDS             complement(9756..10805)
                     /locus_tag="KPD58_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0265672.1"
                     /translation="MEINYKKRNFKSFAKRKNIEISVQRYLIDALSYMALGLFSSLLI
                     GTIFNTLGDNFNISLFTEVISPLAKQVTGPAIAVAIAYGLQAPPLVLFSCALVGACGN
                     ELGGPVGAFVATLFAVEVGKLVSKETKIDILVTPAVTILIGVLVAELIGPAVSAFMTS
                     FGNIIMQATTMQPIIMGALVSALVGIALTLPISSAAICMMLSLGGLAGGAATVGCCCQ
                     MVGFAVMSFRENGVGGLVAQGLGTSMLQVPNIIKNWKIWIPPTVASILLGPLSTTIFK
                     MENIPIGSGMGTCGLVGQFGTVTAMQSAGKGGISMWVGILLLNFILPAIVTLLISEFM
                     RKKGLIKPEDLKLDV"
     gene            11080..12615
                     /locus_tag="KPD58_001904"
     CDS             11080..12615
                     /locus_tag="KPD58_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265673.1"
                     /translation="MIFVKNKKYRKIAIIISIPILLVAQYYLFKFGILRPMVKGVEIE
                     IVDGEYVKDIDKYVVKLNETVTLSTGEYIKVPSYAKNPNIWFNVLDNSGTLKIDGNKM
                     TALKVGYSSVGIMKNSRVLKKATIKVVDPEVESLDMEINGDLKHVGDSATIESTIEVD
                     YDKFKKSYKPVYKSSNENILKIKGNKVSAVGVGKATIYAKCGNKEIERTFRIQARVAE
                     INMDDIKIEVDQNEKLKPDIITSPPGLEPPKIYYELVDSKLPVERAISLSSNGNVVGL
                     REGKDRVRITCGDKSKVITVTVVKETITNDYIQNLVGSHKIVNNKVIIKLEWDYMKDV
                     FDYDIYLKDKLSGESFKKIKSITINESDLGKSNKVYATIEVDMKGNQNIDFDIYVIGK
                     KDSDTTKPSNVINITNGNGNSNNDNNIKDMRVENISANVDRDNNVIRVNWSSISHKDC
                     TYSIYVKNNTNGDGEFTLYQSDIQGNEHTISIPEGDLNLDIYVVANYNGNSSQNSDVI
                     NVR"
     gene            complement(12800..13486)
                     /locus_tag="KPD58_001905"
     CDS             complement(12800..13486)
                     /locus_tag="KPD58_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HBG0265674.1"
                     /translation="MKTIGLIGGMSWESTITYYQVINTVIKERLGGLHSSKCILYSVD
                     FQEIEECQSSGNWEKSAKILADAAIKLQEAGADFIVICTNTMHKVSDKIQESIHIPLL
                     HIADVTATVLREKEIKKVALLGTKYTMEQDFYKNVIINNGIEVLIPNEEDRIIVNDTI
                     FNELCLGIISESSKKAFLSIIDKLGKQGAEAVILGCTEIGLLIKQNDTSIPLFDTTVI
                     HAIEAALSSI"
     gene            13611..14993
                     /locus_tag="KPD58_001906"
     CDS             13611..14993
                     /locus_tag="KPD58_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG0265675.1"
                     /translation="MPINSFENYPMTWKPKLDNRKPPIYKTLAMLLEEDIKRGNLNPG
                     DKLPPQRELADFLDLNLSTITRAFKLCEEKGLICAKVGKGTFISSDVNVSNTLLYQTE
                     SKDIIELGTVHPPYEQNTYIIDFIKNVLKQPEMDRFLQYMSPSGTYMQKKSIAKWIER
                     NNVYTSEENILLSTGGQNAICATLLGLFKAGDRIATDSLSFSGIKSIAKMIGIQLVPI
                     PQENNEISIEYLESYCKNENIKGIYLIPDYHNPTTHTMSDLSRKKIAKIAKQYNIIII
                     EDAINSIFRDGIQTPIFSLASDNTIYIFSTSKFLCAGLRVAFVVAPKRYIENLENALY
                     NMNLMVSPFTAEIVHRLLYSPIIDKIIEEKRDAIIERNEVADKVLSDYNLIGDKNCSF
                     RWLLLPDELDGKSFEISAKNLGVQVYCAERFSVGNSTVPKAVRICVTAPKDVEELEKG
                     LNIIKSLLKS"
BASE COUNT         6024 a         1722 c         2537 g         4931 t
ORIGIN      
        1 catataagat ataaggtgat agatagaaat tgttggatta tgtaaataat taatatataa
       61 taccattatg ggcaataaat acaaatcaaa tgataatata gtctatttat gtaaatgtta
      121 tattgtatcc taagtataat agtaagtttt ttgatagtgg tgtagaaagt aaaaatctac
      181 tagcctaaat tgatgtatgg attagtttag gaacagtaat tgcatatctt aagtttgatg
      241 tcatgttggg ctactagaaa taggtcatct ttttcacttg aatatctttt ctttaaacat
      301 ggggagtatc atatcaaaaa catatgaaaa ttaagtgatt tatgagaaca gtaatatttt
      361 atatgatagt atagaatagt aataaagtat taggaggaac attgagtgtt aaaaaagtgg
      421 tttggtattg tgaaaaaaag acaaaaaagt gagtcagtaa aagaagaagg tgaagtaata
      481 ttaaaaaacg aaaaaatatt atctgaagaa aagttgatag atgaagaagg agttgtagtt
      541 aatattgata atcaaatatt aactaaagta gtaaatgatg acaatgaaat agaagaaaaa
      601 ataatagaag aagatttgtt aattagtgaa aataccataa aattagatga taaagaagca
      661 attattaatg ataaaaacat agaattatgt aaaaaagaag ttcaagttga aagtgaaaaa
      721 ataggtttaa ataagtttga aggactggac caaaatgaaa attataatct agaaaaaaat
      781 gttattgaag aaaaaaaagt aagtgaatgt ttgacagaag aagatttaga gtacataaaa
      841 gaaattaaaa taaaaagagg aaaaagtata aaagctataa acttgtatac taaagaagag
      901 tgggtttttg acactcatat acagtgtagt aaaaaactca aagttccatt agggtacata
      961 agagaaaatt taaaatatgg atatatggat tactttggag atgcaataaa ttatttaagt
     1021 gaagtattaa atatagatga atactgcaaa agtgaatgga gctatctcga taatagcaaa
     1081 tctccatctg agatatttaa tattctaaac aataaaatat ttagtataag gctttctaat
     1141 gaaaaaagaa atgaaatttt gacaaatgat aaaattgaag cattaaaaat gaattataga
     1201 tttgaatgta ttgatgaaga atatgatgaa tattttaaaa aatataagtc tataatcaaa
     1261 agaggtggaa agaaaaaagt tgaattagta aataaaaaag gtgacatttt agaaatattt
     1321 aagtctttag aagaatgtgc catttatttg caaaaggaaa agaatgaagt tatacaaatg
     1381 ttaaaatatg gagatacaaa agtaggaaga aactttataa ggtatagttt gagaagtatt
     1441 taatttcatt ttaaattcat aagagtttca tttctgattc acacatttaa tattatttta
     1501 atgtaaaata ataaaggtga agtattgcac aattaaaatt aaggagggta ttaatgtctg
     1561 aagaaaaaaa tccaaaagaa aatacattag atgaatctaa aaattcagat aatgtaaatt
     1621 ctaaaaaaga tgatgtgtca gatgaaaaaa atactgataa caaagttaaa aaaagggaaa
     1681 taaagctaaa caacaaaaca cttaaagcta ttgtagcaat tgttgcagta ggagctatat
     1741 gtttaggtgg aggctatgtt tttggcaaag aagctggaag aaaattgcct gctacttcaa
     1801 aacactataa tagcagtgag gtagtagcta ctattgggga taaaaaaata acaggtgaaa
     1861 tgttaagaaa gagaatggag ccattatttt atttaaatgc aaaaactact atgactgatg
     1921 acgaaatacg ttcatatgaa tcaaatatga ttgaaactat gattacatct gatatattat
     1981 cctctgaagc agcaaaagag aaaataagta taactaatga ccaactagat gctcagtata
     2041 ataatttaat gagtagtata gaatcagcaa tgggaatgac taaagaagag tttttaaaac
     2101 agtttgattt gactgaaaaa tatataaaag atgatatgaa aaaagagcta gtagcatcaa
     2161 aatatttaaa tgaaaaatca aaagttactg ataaagaagc agagaactat tacaataaaa
     2221 acaagagcaa ttatttacaa gttagagctt cacatatttt aataaaaact gttgatgata
     2281 aaggtaagca agttagtagt agcaaaaaag ctgaacttaa gaaagaagct gaagaaatac
     2341 ttaaaaaagc ccaagctggg gaagattttg ctactctagc taaaaaatac tctgaagata
     2401 gtagtgcaga atcaggtgga gatttaggtt tctttggtaa aggtcagatg gtggaatctt
     2461 ttgaaaaagc agcctttgca ttgaagaagg gagaggtatc taacaaactt gtagaaagtg
     2521 attatggata tcatataatt aagaaaactg atgaaaaata tcagccattt gaagaggtaa
     2581 aatcagattt agtaagcagt ttaacatcag aaaaacaaaa cctacttata caaaatctaa
     2641 aagaaaaata taatgttaaa attacagata aatatcaaat gaaagctaaa gaatagataa
     2701 agtcaattta ttaaaaaaaa gaaaaattaa ctgtatggat aaaagctatt tttactctca
     2761 atatagagag tataaaatag cttttattat tttgtaataa aatctattta tatatatgaa
     2821 gtgataaaga aataacatgc aaaaaggtat taaaaagaaa tatttttaat ttgaattttt
     2881 tcaataaaaa tttacaaaaa tgaaaaaata aacaaaaaag aattgaagat ttaagttgtt
     2941 atttaaatat atatatatta agattttctg ctgaagatac taaaaaaata atatttttca
     3001 taattttaaa aataataata aaaaaatgaa aatacaggct ataatctatt gagtaaacat
     3061 agaaaagatg atattataag tatgtgctaa aatggttaaa gcaatattaa tattaaattt
     3121 aactataaaa caaaaggggg cgttttttta tgtcaagtac agaaagcgta tctaaaaaaa
     3181 gtggtcatcc gaagagtttt tggcttattt gcatgacaat tttgtgggaa agatttagtt
     3241 attatggttt aacagcttta ttggtattat tttttacagc aagtgtagct caaggtggtt
     3301 taggtctttc tactcaagaa gcaacatcta tgtttggctt atttacaggt cttatatatc
     3361 ttacaccatt agtcggtggc tggttagcag ataaagtttt aggtcaacaa aaatgtattt
     3421 ctataggtgc acttttaata gcaataggag atttcgtact atttgcttca tctacatcta
     3481 ttgcaatggt ttgggtaggt ctatcaataa ttattttagg aaatggtttt tttaaatcaa
     3541 gttgctcaaa tatagttggg gatatatatc ctaaaactga tgtgtcaaga aaagatgcag
     3601 catacagttt attctatatg gcagtaaatg taggagcatt tatagctcca ataatatgtg
     3661 gattgctttc tgataatgta tttgcagtaa gaggagcaag tggagatata gtatcttatg
     3721 gatataagat ggcattttta atttgtggta taggcatgat gttaggtatg attatattta
     3781 cacttcttgc accaaaatta ttaaatgaag tcggaaaata tcctgtgact aaaggtaata
     3841 aaggtgaaaa aattgagtat ggaccactta ctaagttgga gaaaaatagg ataatagcaa
     3901 tggttatttt atttttattt gtggtcttat tctggacagc atactatcaa acacaaagtt
     3961 cttttatgat ttatgtaagg gataatgtaa atagaactct atttggattt gaaatgccag
     4021 tagcatggct tacatcacta aatgctatat tatgtgtggc tttatgccca gtattagcta
     4081 ccctatgggt taaattatct cataccaaaa gaggtgattt ctctattcca gttaaaatgg
     4141 gattaggaat gataataatg ggtgttgggt ttatagttat ggtatttgct acattagcta
     4201 atggtccaga tggaatggta aaggctagta tattgtttat agtattgact tatatattta
     4261 caacagtagg tgaattattc ttatctccta taggacttgc aatgttcaat aaattagctc
     4321 cagctaagta tgctacgttg tctatgggag cctggtatct aacaagtttt tttgcaagtt
     4381 tattatcagg aaagattgct ggttttacag ccacacttgg ttttttacag atatttggag
     4441 gaatagcaac agttttaata atatttggtt taatattatt gggaataaga aatgtattaa
     4501 caagattaat ggctatggat ttactaacaa aagaaaaata attcatgacg aattattgta
     4561 aataaatgaa ttactttaaa taaaggaagt tacatttaaa tataaaatta aatgtaattt
     4621 cctctttgat tatgaaaaaa agctttacac aaatagatat tataattaaa attataattg
     4681 tgataattag agtgtataag atttatataa attatacaaa aaatatttat gtaataaact
     4741 ttacatactt atatatttag ttattcaaac tattatttcg aggaggtctt gttgtgaata
     4801 taaaagatag attaagtgga ttaagaaaat ttatggaaga aaaaaatata gatgcctata
     4861 tgattccatc ttctgataat caccaaagtg aatatgtagg agattacttc aaatcaagag
     4921 aatttatatc agggtttaat ggttcagcag ggacggttat tgtgactaaa gacgaagctg
     4981 ggttatggac agatggaaga tattttatac aggcagaatc tcaattagaa ggaagtacaa
     5041 taaaattatt caaaatggga caagaaggtt gcccaactac agatgaatat ttatataaaa
     5101 atattcctga aggaggaact ttaggatttg atggtagagt tatatctgct agagaaggag
     5161 ctacattagc tgaaaaatta tctaaaaaag gtataaagat agaatatcag tatgatttaa
     5221 tagatggtat atggccagat agacctgctt tatcagatag taaagcattt ttattagatg
     5281 ttaaatattg tggagaaagt tttagctcaa aattagctag attaagagaa aaaatgagtg
     5341 aaaaaggtac ttctactcat gtaataacta ctcttgatga tatagcatgg ttattcaata
     5401 taagaggtgg agatgttaaa tataatccag ttgtactttc ttatgcagta ataactttaa
     5461 aagaagttta tctatttgtt gatgaaagta agttaaatga agaaatacta aatgaactag
     5521 ctaaggaaaa tgttcaaata aagccatata atgatgtgta tgaatttgta aaaaatatag
     5581 acaaaactga aaaagtttta ttagatggaa ctaagcttag ttatacaatt tacaataata
     5641 taccttgtga agttgagaaa gttgatgagt tcaatccagt aatgtttttt aaagctcaaa
     5701 aaaatgaggt agaattagaa aatataagaa attcacatgt aaaagatggt gtagctttca
     5761 ctaaatttat gtactggtta aagaaaaatg taggtaagat ggaaatcact gaaataagtg
     5821 ctactcaaaa attagaggac ttaagaagag agcaagaagg tttctttgag ccaagtttca
     5881 atacaattgc tgcatataaa gagcatgctg caatgatgca ctatagtgca actccagagt
     5941 caaattataa attagaggca gaaggattat ttttagtaga ttcaggtgga caatattatg
     6001 atggaactac tgatataact aggactacag ttttaggacc tataagtgat gaattaaaac
     6061 tacactttac ttctgtagca agaggaatga taaatctttc taaggctaag ttcttgcatg
     6121 gttgtagagg atataactta gacatactat ctagaagttg catgtggaat atgggaattg
     6181 attatcaatg tggaactgga catggtatag gatttgtgct aaatgttcac gaagctccaa
     6241 atggatttag atggagagta gtacctgaaa gatttgatag tgcagttctt gaagaaggaa
     6301 tggtaactac taatgaacca ggtatatata tagaaggttc tcatggtata agaacagaga
     6361 atgaaattgt agttagaaaa gctgaaaaga atttctatgg tcaatttatg gaatttgaag
     6421 ttgttacgtt agctcctatt gacttagatg gtatagtacc agaattaatg aataaggatg
     6481 aaaaagatta tttaaactgg tatcataagt tagtttatga taaaatatca cctttcttaa
     6541 ctgatgaaga aagagaatgg ttaaaagttt acactagagc tatatagttt gtactagata
     6601 caataaaaat cgaggtatta taatgcctcg attttttatt gtatatttta ttaactttaa
     6661 aaaattttta atttgataat tctactaatt ttttattcat tataagtagt ataattccaa
     6721 atatcaataa aaatgatggt atcactatga agacttgtaa cataccaagt tggtctataa
     6781 tagcttgcgt atagccagat aatttatttg ctgcaaattt gcctaaaaac caaacaccca
     6841 ttaggaatga aatatacttt tcaggcgaaa gtttatttac cataccatac ccgactggag
     6901 aaaagcaaat ttctcctata ctttggaatg ttaaaaatcc aactacccaa aatacacttg
     6961 ctttgtgaat tgaattaggg tctgttcctg tttgtataac tgctactatc ataaacaaaa
     7021 atgctatggc taatagtata aaacctaagc tcattttttt tggcacactt aaatcgcctt
     7081 ttttagtttg agataatttt acccatacta aagcagagat aggtccaaga ataacacaaa
     7141 ggagaccatt gtatgaatct atccatgaag taggaatttg aaaattgcca acagtaagat
     7201 ttatgtgttc ctttgtatag agtgcaatag acatagaggc ttggttatat gctatccaaa
     7261 aaaatactgt gaaaaaagct aacaccaata ttactattat tctttttttc tcaatcaaag
     7321 ttaaaggtct tgaagcgagc attttcttat attctagtga ttttcttata gactctctcc
     7381 cgttaatagg gtcattattc aaaggtttat aaaactttct gtttccaaat ataaagaaga
     7441 ataaacctat tagaaccgta attgatgaaa ataaaaatac atatctatat ccatacatac
     7501 ttatagataa tccgcttttc atagcaaacc attcattagc aactaaaccg catattaaag
     7561 ggccaaataa agtacctaga ttagtagcca tatacgtaat agagaaagct ccatcttttc
     7621 ttgggtcatc attggtatat aagtttccaa ccatagtatg taagtttcct ttataaaaac
     7681 ctattcctaa tccagataaa aatatcatga tgtttatatc catcttacta tttgctatgt
     7741 atcctagaaa ataaccaatt gaaaccatta gcattccaag tataatacag tttttgggac
     7801 ctaaaaatct atcactgacc catccaccaa taactggaga gaaataagtc catgctaaaa
     7861 aagatgcaag cattgatgcg gattctgctg gtgttaaccc taaaccacct ttggagactt
     7921 gttcagctaa aaacaataat aataccaatt tcataccata gtatgcaagt cctacaaagg
     7981 taaatattga acagcagaaa tataaatctc taggttgttt aagtttttct tgcgttttta
     8041 aaactgacat atgtcgagaa cctccagata aatagtttaa caaaatttta ccatactata
     8101 tgagtatatg tttttttcat atagacgtca acttgtaaga taatttaatt aagtcgaaat
     8161 atatgtaaaa atagtatcat ttttgtgtat tcagtaatat ttttttatga taatatatgt
     8221 tattattata ttaaaataca aacatattta tggaaaggat atgccaattt agtagttaga
     8281 ttgattgttt ggcaggtggg atgagctatg ataagaaaga aatctgaatt acaagtagaa
     8341 gtaaaagaaa atttaaatgg tggtatagga aaggttaagc ttgaaaacat aatacaaaat
     8401 gaagagctta aaggaaaagg aagactattt aaaagagtag ctttaccagt aggaagttca
     8461 attggtgtgc atgaccatac aacagatttt gaagtatatt atatactaaa gggaaaaggt
     8521 aaagtatttg ataatgggga atttgtagaa gtaaatgagg gtgatgtagt gtatacagct
     8581 gatggcgaaa aacattctat agaaaatata ggagaagaag atcttgagtt tgtagcacta
     8641 gttttatata tgtaaaatat ggaggaggag tcatgacagg caagatagaa gaaattaagg
     8701 acaagataaa tagattttgc aactccaatt taaatcaaga atacaaagat ataagcttta
     8761 aaattttgca aaacctttta gataataata aagagataat tcattcttct agagctgata
     8821 tatggtcttc agctatacta aatatagttt tagaacaaaa tcttttatac aataagaagc
     8881 atccactaca tataacaaaa aaagagtttt caaaacaaat aggtgtttct ttaaatacta
     8941 taaataataa atcgtcttta ataaaagatg tttgtagtat tgacttttta aatcaagata
     9001 ctatagctat ttctaatgta gaattttggg taaaagagac taattccaaa tcaaagtctg
     9061 tagatttaga tttagaaaag aagaaaatac tatataaaag atatattaag ctatcacaag
     9121 atgcgaatag tgatattgaa tcaattagat atatggagga agctgtaaat ataggtaaaa
     9181 gtatgattac taaagaagat aaagaacttg gattttggaa aggtttatct accagagcct
     9241 atatgatagc acttgaatcc cttgctagaa agttagaaag tattaataat ctaaaggagt
     9301 ccaaaaaagt attagaatat ttaatagaaa ttaatcctag tgatgagcag ggaataagat
     9361 acaaattatt aaatgtatta attagattaa atgatagaac ttcaataaat aaattatttg
     9421 aactttataa agaagaaaaa tctgctacat ggatgtacag taaagctctt tattacttta
     9481 aaaataaaag tatgtttttg gcaaatgatt ctattaaggt tgctagaagt aaaaataagt
     9541 acgttggatt atatttgtta gattggcgaa atgcttttgg tagagaattt gttacagagg
     9601 aagaaaaggc agaagctgtc tattactatg atgaaaacat agtgatttgg aatgaagtta
     9661 aaggttctat ggattggcta ttaaaaaaaa tgttggaatt ttcataaaaa aaactactcc
     9721 ttacttttat ataagtaaaa ggagtagttt ttaatttata catctagttt taaatcttca
     9781 ggttttatta gacctttttt tctcataaat tctgaaatca acaaagttac aatagcagga
     9841 agtataaaat taagtaaaag tataccaacc cacatagata tacctccttt tccagcactt
     9901 tgcatagcag ttactgttcc aaattgtcca accaatccac aagtacccat accagaacca
     9961 ataggaatat tctccatttt gaatattgtt gtagataatg gtccaagtaa tattgaagct
    10021 acagtaggtg gtatccaaat tttccaattt tttattatat taggtacttg caacatagat
    10081 gtcccaagac cttgtgccac aaggccacca actccatttt ctctaaagct cataacagca
    10141 aagcctacca tttggcaaca acaacctaca gttgctgcac caccagcaag accacctaaa
    10201 cttaacatca tacaaatagc tgcactactt ataggtaatg taagagctat tccaacaagt
    10261 gcagagacta aagctcccat tattataggt tgcatagttg tagcttgcat tattatattt
    10321 ccaaatgaag tcataaaagc agaaacagct ggaccaataa gttcagctac aagtactcca
    10381 attagtattg tcacagcagg tgtaaccaat atatctattt ttgtttcttt tgaaactaat
    10441 tttccaacct ctacagcaaa aagtgtagcg acaaatgctc caacaggtcc tcctaattca
    10501 ttaccacaag ctccaacaag tgcacaagaa aatagtacaa gtggaggtgc ttgaagtcca
    10561 tacgcaatag ccactgctat agctggacca gtaacctgtt tagcaagagg gcttataact
    10621 tcagtaaaaa gagaaatatt gaaattatct cctaatgtat tgaaaattgt tccaattaaa
    10681 agggacgaaa aaagaccgag tgccatataa cttagtgcgt caataagata cctttgaaca
    10741 gaaatttcaa tattttttct tttagcaaat gatttgaaat ttcttttttt ataattgatt
    10801 tccaatttaa aatcctcctc atataatata agtataattt ctttttaatt aattatataa
    10861 cttttcagct gacaagacaa atgttatttc actaaaatta gactattgta atagtaagaa
    10921 attaacatta attttagttt taaattttaa atttatggtt tataattagt atagggatat
    10981 tctgaaatat aataattttc cagtttatcc caatatttaa cttaggtaga ttgcttacaa
    11041 aacaaatgat tatatattag gctaaaagga agtggataga tgatatttgt aaaaaataag
    11101 aaatacagaa aaattgccat aattatatct attcctatat tgcttgttgc gcagtactac
    11161 ctttttaaat ttggaatact tagaccaatg gtaaagggag tagaaattga aattgtagat
    11221 ggggaatatg ttaaagatat agataaatat gtagtaaaat taaatgaaac agttactttg
    11281 tctacaggtg aatatattaa ggttccttcg tatgctaaaa atccaaatat ttggtttaat
    11341 gtattggata actcagggac tttaaaaata gatggaaata aaatgacagc attaaaggtt
    11401 gggtattctt ctgtgggtat catgaaaaat tcaagagtat taaaaaaggc tactataaaa
    11461 gtagtagacc cagaggtaga atcactagat atggagatta atggggattt aaaacatgtt
    11521 ggtgatagtg ctactataga aagtacaata gaagttgatt atgataagtt taaaaaatca
    11581 tataagccag tttataagag tagcaatgaa aatattttaa aaatcaaagg aaataaagta
    11641 agtgcagttg gagttggtaa agctacaata tatgctaagt gtggaaataa agaaattgag
    11701 agaacattta ggattcaagc tagagttgct gaaataaata tggatgatat aaaaatagaa
    11761 gttgaccaga atgaaaaatt gaaaccagat ataataactt ctccacctgg tttagaacca
    11821 ccaaaaatat attatgagtt agtagattca aaattacctg tagaaagagc aataagttta
    11881 agtagtaatg gaaatgttgt agggcttaga gaaggtaaag atagggtgag aataacttgt
    11941 ggagataaat ctaaagtaat aacagtaaca gtagtcaaag aaactataac gaatgactat
    12001 atacaaaact tggttggttc gcataaaata gtaaacaata aagttattat taaacttgaa
    12061 tgggattata tgaaagatgt ctttgattat gatatatatc ttaaagataa gttgtcagga
    12121 gaatcattta agaaaattaa atctataact ataaatgaaa gtgatttagg taagtctaac
    12181 aaggtatatg cgactataga agtagatatg aagggtaatc aaaatataga ttttgatatt
    12241 tatgtcatag gtaaaaagga ttcagataca actaagccaa gtaatgtaat aaatattacc
    12301 aatggaaatg gaaattcaaa taatgataat aatattaaag atatgagggt agaaaatata
    12361 tcagctaatg ttgatagaga taataatgtc attagagtta actggagtag tatatctcat
    12421 aaggactgta cttacagtat atatgtaaaa aataatacta atggagatgg agaatttact
    12481 ctatatcaaa gtgatataca gggaaatgag catactataa gtattcctga aggagattta
    12541 aatctagata tttatgttgt tgcaaattat aatggaaata gctctcaaaa tagtgatgtt
    12601 ataaatgtaa gatagatttg tatacaaatt gaaagtgttg agtttattta aatattaaat
    12661 taagaaaaag gatagtatag ggtaattata ctatcctttt ttgttacaat atataggttt
    12721 aaaatctcta gtaatataaa ttataaaaag tcttatttac acaaatctat agatattttt
    12781 tttattaatc aatattgtct taaattgaac ttagtgcagc ttctattgca tgtattactg
    12841 tcgtatcaaa tagtggaata gaagtgtcat tttgtttaat taaaagacct atttctgtac
    12901 atcctaatat aacagcctct gcaccttgtt tgccaagctt atctataata gataagaaag
    12961 ctttttttga agattcagat ataattccta agcaaagttc attaaatatt gtgtcattta
    13021 ctattatacg gtcttcttca tttggaatta agacttcaat tccattatta ataattacat
    13081 ttttataaaa atcttgttcc attgtatatt tagtacctaa taaggccact ttttttattt
    13141 ctttttccct taataccgta gcagttacat ctgctatgtg aagtaatgga atatgtatac
    13201 tttcttgtat tttatctgat accttatgca tagtattagt acagataaca ataaaatctg
    13261 cccctgcctc ttgtaattta attgcagcat cagctaaaat tttggcgctt ttttcccaat
    13321 ttccactaga ttggcattct tcaatttctt gaaaatctac actgtaaagt atacatttag
    13381 aagagtgtag tccacctaat ctttctttta ttactgtatt aataacttga taatatgtaa
    13441 tggtactttc ccaactcata ccaccaatta agcctatagt cttcatatta tacctctctt
    13501 atttatttat tttgaaatag ctatttacaa atacaagtat agcatctaaa atagaagcat
    13561 acaataataa aattgtatgg agtacaatta taattaagag gagtaattat atgccaataa
    13621 attcttttga aaactatcct atgacatgga aaccaaagtt agataaccga aaaccaccaa
    13681 tatataaaac tttagcaatg ttattagaag aagatattaa aagaggaaat ttaaatcctg
    13741 gagataaact gcctccacaa cgggaattag cagattttct agatttaaac ttaagtacaa
    13801 taacacgagc atttaaactt tgcgaagaaa agggactaat atgtgcaaaa gtgggaaagg
    13861 gaacattcat ttcttctgat gtaaatgtaa gtaatacatt attatatcaa acggaatcaa
    13921 aagacataat tgagttgggg acagttcatc ctccgtatga gcaaaataca tatattattg
    13981 actttattaa aaatgtacta aaacaaccag aaatggatag gtttttacaa tacatgtctc
    14041 catctggcac atatatgcaa aaaaagtcaa ttgcaaaatg gatagaaaga aataatgtat
    14101 acaccagtga agaaaatata ttattatcta caggagggca aaatgctata tgtgcaactt
    14161 tactaggatt atttaaagct ggtgatagaa ttgctacaga ttctttaagt ttttctggaa
    14221 ttaagtcaat agctaaaatg ataggtattc aattagtccc aattcctcaa gaaaataatg
    14281 aaatatcgat tgaatatctt gaaagttatt gtaaaaatga aaatataaaa ggcatatatc
    14341 ttataccaga ttatcataat ccaacgacac atactatgtc tgatttatct cgtaaaaaga
    14401 tagccaagat agcaaagcaa tacaatataa taattattga agatgctata aatagcattt
    14461 ttagagacgg tatacaaact cccatatttt ctttagctag tgataatact atatatattt
    14521 ttagtacatc taaattttta tgtgctggtc ttagagtagc atttgtagtt gctccaaaac
    14581 gatatataga aaatttagaa aatgcactat ataatatgaa tctaatggtt tctccattta
    14641 cagcagaaat agtacaccgt ttattatatt ctcctattat agataaaatt atagaagaga
    14701 aaagagatgc aattatagaa agaaatgaag ttgctgataa agttttgtca gattataatt
    14761 tgattggtga taagaattgc agttttcgtt ggttacttct tcctgatgaa ttggatggaa
    14821 aatcatttga aatttctgca aaaaatctag gagttcaagt atattgtgct gaacgatttt
    14881 cagtaggaaa ctctacagtt ccaaaagcag ttcgtatttg tgtaacagct ccaaaagatg
    14941 tagaagaatt agaaaaaggg cttaatataa taaagtcact tcttaagagt taaatttatt
    15001 ttactattat ttaattaaat aaataaaaag gcggagaaat ttattataat aaacctccgc
    15061 ttttattatt taatgaaata ctttactttt tattaaaaaa tttaatttta ataatcttga
    15121 aaatataatc gttttatggt aaaataattc ttaaaaatag aaaaaattaa tcatttgtta
    15181 aaaaaaatgc ataatttatt gtaatttatt gacg
//
LOCUS       DAELLV010000079        15134 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.079, whole genome shotgun sequence.
ACCESSION   DAELLV010000079 DAELLV010000000
VERSION     DAELLV010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15134
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.079"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            46..612
                     /locus_tag="KPD58_001907"
     CDS             46..612
                     /locus_tag="KPD58_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HBG0265676.1"
                     /translation="MRKLEEILEYNKSFVENKEYEQYVTSKHPNKKIVVLSCMDTRLT
                     ELLPKAMNLKNGDVKLIKNAGATIMHPFGSIMRSVLVAIYEFDVDEVMVVGHHGCGMC
                     NVDTDKLLGKILDRGISNDVILTLRSAGIDTKQWLHGFDSAEESVKDSIDLIKGHPLI
                     PDGIIVHGLIISPETGKLDVVVNGYEDK"
     gene            917..1378
                     /locus_tag="KPD58_001908"
     CDS             917..1378
                     /locus_tag="KPD58_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0265677.1"
                     /translation="MNSNSEEKLNDVFLQLMRIYFIRTHFYFDKLGLYHGQPQILFSL
                     KMKDGQSQKELADKRKVKASTTTVMIKRMEKAGLVERKQDEKDQRVSRIFLTDKGLEI
                     CNKVEEFNKELEREFFLGFSEEEKIVLRRLLMQVRDNLKAGCHKHEDEKYF"
     gene            1446..3692
                     /locus_tag="KPD58_001909"
     CDS             1446..3692
                     /locus_tag="KPD58_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0265678.1"
                     /translation="MFKLIKYLKKSALSIVIIVCLLVIQAVCDLSLPEYTSNIVNVGI
                     QQGGVENSVPSVIRESELNKITLFMDKSSKDKVLDNYTLLNKKDYVKYKDKYPGLKDE
                     SLYELNTKDKDTIDDLNVIFGKAILIVSGLEGDSKEVKAMKAQLMSKLPPQATQSGDV
                     DIFKLLSAMPKEQLDTLTKEMSKGFESMPESMITQSSVSYVRSEYEKIGIDTEKTQNN
                     YILFTGAKMLGIAMISMVATITVGFLAARVAASVGRNLRSGVFRKVMSFSNTEMNEFS
                     TASLITRSTNDIQQIQMLMVMLLRIVFYAPIMAIGGVIKVLNTNTSMAWIIAVAVVAI
                     LSLIVVLFSVVMPKFKLVQKLVDKLNLVSREIITGIPVIRAFSTEKHEEERFDKANKN
                     LTKTNLFVNRVMTCMMPAMMLIMNLITVLIVWRGAYSVDSGAMQVGDMMAFIQYTMQI
                     IMSFLMISMVSIMLPRASVSATRIDEVLVSDISIKDPEKSEAQEFKEDKKGLVEFKNV
                     SFMYPDAEEPILTNISFTAKPGETTAFIGSTGSGKSTLINLIPRFFDVTEGEILLGGV
                     NIKNVSQHDLREKIGYVPQKGVLFSGTIESNLKYGRKDATDEEMRTAAEIAQATEFID
                     SKPEAFKTEISQGGTNVSGGQKQRLSIARAIAKNPEVYIFDDSFSALDLKTDAALRKA
                     LKSKTAESTVLIVAQRISTILNAEQIIVLNEGEIVGIGTHKELLKNCEVYEQIALSQL
                     SKEELANE"
     gene            3685..5517
                     /locus_tag="KPD58_001910"
     CDS             3685..5517
                     /locus_tag="KPD58_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0265679.1"
                     /translation="MSRMGRGPMGKSMGAGQKANDFKGTMRKLIAYLSKFKISIILVI
                     VFAIGSASFSIVGPKILGKATTKIFEGLVSKVSGGNVGIDFNAIGKILTFLLFLYLIS
                     ALFSFIQGFIMSGISQKVSYNLRKEISAKLDRLPMKYFDTKTHGEILSRITNDIDTLN
                     QSLNQSMTQLITSVTTMIGVLIMMLSISGIMTLVAVLILPISMFVISRIIKKSQKYFR
                     YQQEYLGNVNGQVEETYSGQTIVKAFNREDEVIEEFDKLNDSLYNSAWKSQFLSGIMQ
                     PLMMFIGNLGYVMVSILGGWLAIKKTIEVGDIQSFIQYVRNFTQPMTQIAQVANLLQS
                     TAAASERVFEFLEEEEEVQIVENAVSIDGLEGKIDFENVNFGYNPNKTIINDFSVNVK
                     PGQKVAIVGPTGAGKTTIVKLLMRFYDVNSGSILIDGHNIKDFNRSELREMFGMVLQD
                     TWLFSGSIMENIRYGKLNATDEEVIEAAKSAHVHRFIKTLPDGYKMKLNEEASNVSQG
                     QKQLLTIARAILADPKILILDEATSSVDTRTEVLIQKAMDNLMEGRTSFVIAHRLSTI
                     RDADMILVMNEGDIVEQGNHEELLKKGGFYANLYNSQFEEDEAM"
     gene            6236..7513
                     /gene="hflX"
                     /locus_tag="KPD58_001911"
     CDS             6236..7513
                     /gene="hflX"
                     /locus_tag="KPD58_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBG0265680.1"
                     /translation="MDMAKKGITVGLNINNKSEDFNELMIELENLCSACDIDVVGSIT
                     QNAKQVNRAFYIGTGKVEEILNLIKKENIEIVIFYNELSTSQLKNLEEKLNCEIIDRT
                     ALILDIFAQRAKTREAKLQVELASLKYMLPRLIGSNENLGRQSGGVGTKNRGSGEKKL
                     ELDRRRIEEKITSLNRELDDLKFQRETQRSMRRKSNLPNVALVGYTNAGKSSIMNKLV
                     DIFKNSEEKKVFEKNMLFATLETSVRNIVLANNKEFLLSDTVGFVSNLPHDLVKAFRS
                     TLEEACEADVLLHVIDISNPSYKSHIKVTEDTLKQIGADGIPMIHVYNKIDLIDVEVL
                     DRILDSIDKEGIFVSVKKDINIDKMIKCICDSIFKDYVRYKFLIPYDKGHVVSYFNEN
                     TSIINTEYREDGAILDVECSNIEYNKYKKYALE"
     gene            7746..8243
                     /locus_tag="KPD58_001912"
     CDS             7746..8243
                     /locus_tag="KPD58_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0265681.1"
                     /translation="MDAESLMKKYMVDDDSRKQAIFASIFVMQNKLQTTCDKLDPELT
                     MKQWLLLAIADSVDETLTLTKLGELMGCSRQNVKKLAIALEKKGFIEIEQNEKDSRAI
                     CLVTKDRVQEYSKKVRGMQEKVLQLLFEDFTEKEAEQLFFGIMKLSIGIENVKNYVEN
                     NDVGE"
     gene            8292..8816
                     /locus_tag="KPD58_001913"
     CDS             8292..8816
                     /locus_tag="KPD58_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBG0265682.1"
                     /translation="MKSVVVIYKSKYGSTKKYAKWLSELLSCDIFETKNINIEKVMKY
                     DTIILGGGLYACNIAGISFLKKNYKELKKKNLVVFGVGAAPYDDEVIKKLREVNFKDE
                     LNKIPCFYCRGAWDETKMSFIDYTLCSFFKKSIAKKERQGLKPAEAPLLSGEAFDWCN
                     KENLTPILDYIREL"
     gene            9128..10492
                     /locus_tag="KPD58_001914"
     CDS             9128..10492
                     /locus_tag="KPD58_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HBG0265683.1"
                     /translation="MDIKELVKMQRKYYNTGKTRDISFRIEQLKKLKLVVSQNEEKIL
                     LALKKDLNKSDFEGFMTEVGMFYSELNFAIKNIRKWSKIKRVKSSMVNFPSISKVVPQ
                     PYGVTLIMSPWNYPFQLALIPLVWSLAAGNCVILKPSEYSVSTSSVVKDIVEDTFSKE
                     YVAVVQGSQEESEKLLLERFDYIFFTGSTNVGKIIMKSASEHLTPITLELGGKSPCII
                     LKDANIDLTAKRLTWGKLINAGQTCVAPDYVLVHEDRKNELIEKIKYYTNKYFGDNPC
                     NNEQFPKIINQRHFNRILSLIDKDKIVYGGNYNKETLKIEPTIVDNVNWDDNIMKEEI
                     FGPIFPILTYKDLDEVIQKIIQMPNPLALYIFTKNKYLENKLLEMISAGGCCINDTVT
                     HIATNYLPFGGIGESGMGSYHGKAGFDTFTHYKSVLKKLNLDVPIRYAPYDNKLIKVL
                     KKIM"
     gene            10631..11797
                     /locus_tag="KPD58_001915"
     CDS             10631..11797
                     /locus_tag="KPD58_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG0265684.1"
                     /translation="MKINWNTKYTTIAIYTFIIAASSIIFYLVSSQIDVFSNNLDAIF
                     TTLQPFIIGFAIAYLLNFILKFYEDRIFIKSEKLKKLKQSSKRGLGLLLTYATATLIL
                     YLFMHFVLPQVIESIVGLANDIPMYVNNATKLIDKLMTDLNLDEQYFNLAVDKWNEFV
                     TYIIKFVTDLIPILGSMLKNVASSIWNIVLGLIVSVYLLIDKEKFYGLSKKITYAVFT
                     EKQAARILELTHRSNYTFGRFLGGKILDSFIIGILTFVILTLVKMPYTLLISVIIGIT
                     NIIPFFGPLFGAIPSTLIVMFVSPIKAFWLLLIILIIQQIDGNIIGPKILGDSIGISA
                     FWILFSLLVAGKLLGFIGMVIGVPMFAVIYSIIKDIVESKLDKKGLPTDTSDYM"
     gene            11914..12696
                     /locus_tag="KPD58_001916"
     CDS             11914..12696
                     /locus_tag="KPD58_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBG0265685.1"
                     /translation="MAFIECKFKSKYTGGETDIIVILPVANGKELFSGKEIYDFEGKF
                     KTLYLFHGLEDDQSSWIRYTNVEHYAKNKNIALVIPRVETSFYTDMAYGHQYFTFVSE
                     ELPKFVRAIFPLSDKREDNFVAGMSMGGYGALKLALSKPKEFSAVASFSGSPDMIHEL
                     ENQQIETNADILFHDFGRPEDARNSENDLLYILKNLKERNEDIPKIYQFCGDRDFLYK
                     NNQMFKKYADSLGVDVEYEECVGGHEWRLWNLWIKKFIDIIG"
     gene            complement(12815..13468)
                     /locus_tag="KPD58_001917"
     CDS             complement(12815..13468)
                     /locus_tag="KPD58_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0265686.1"
                     /translation="MSKSEKGKETKASIISAARELFFEQGYHKTTTRQIAERANINLG
                     LISYYFSSKSEIGAIIYEDIRNEMQSLIYNYHEEGTMMMFFLAMNADLCFLLKNEAYR
                     NFYLDIAYEEAILSYQRSVTEMVMKRYVNDKESIEHDELILSCIGILAMKPEIIRVYT
                     SHKYDISSQAVIKHFTKQFLINLGHSTDLCEEILEELSKYYIDIVDNFTPIITRVRT"
     gene            complement(13492..14856)
                     /locus_tag="KPD58_001918"
     CDS             complement(13492..14856)
                     /locus_tag="KPD58_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HBG0265687.1"
                     /translation="MLLLIVTCAIYIWIGFYFSKKVKTASDFLIAGRNLPLPILAATI
                     AATSFGGGCLVGGVQWGAERGLWVGMYSTIGAALACFINAFFAGKLRSLSCDITPADY
                     IETRYGHSVFLRAYQSLVTPISILAIMGSQLISFGSVSTAFGIPYDIAVIIGVIVIII
                     YTYTSGMWGVAVSAFIQLAICIVFLPIVAYISLKLVGDNPLLMLQSMVKEPFFPGNKS
                     ISDFMYTVLPLVLGSIFSYESFLRYQCADNAKNAVKSSVIAGFILLFLAFPIGIIGAI
                     GGSLFPNISSVQVLPQMISTTLPPLISVLFLAAILAAIMSTADSLMTSMSAIISRDIY
                     NKLLHPNIEFNDLKNTLKYSQIASALTAIIGAIIALKFDSLLELLFWPAPLCTGVIFA
                     PFVIGLCWKGATKKGAIYAVIVSVILALLNMIGILTVFDRILVPIIGGSITIFIVSKF
                     SKKD"
BASE COUNT         5877 a         1663 c         2556 g         5038 t
ORIGIN      
        1 agtgatataa taataaaaaa atttttaaag agggtgatat tacatatgag aaaacttgaa
       61 gagatattag aatataataa atcatttgta gagaacaaag aatatgaaca atatgtgaca
      121 tcaaaacatc caaataaaaa aatagtagtc ttatcttgta tggatacaag attaactgag
      181 cttttgccta aggctatgaa tttgaaaaat ggagatgtga aactaataaa aaatgcagga
      241 gctactataa tgcatccttt tggaagtatt atgagaagtg ttttagttgc aatttacgaa
      301 tttgatgtag atgaggttat ggttgtaggg catcatggat gtggtatgtg taatgttgat
      361 acggataagt tacttggtaa aattttagat agaggaatat ctaatgatgt aatacttaca
      421 cttcgtagtg ctggaattga tacaaaacaa tggttacatg gattcgattc tgcagaagaa
      481 tctgtaaaag atagtattga tttaataaaa gggcatcctt taattccaga tggtattatt
      541 gtacatggtc taattatatc acctgaaact gggaagttag acgttgttgt aaatggatat
      601 gaagataagt agagtatcat aatgttatat aatcaagata ctgttgaata tgatgttgtt
      661 tcaaaattta ttttgggaca acttttttat ttgaaaatat atgaaaaggt ttattttaag
      721 ctttgcaata aaaaatataa gtagctgact aattaaaaat cttactatta tttattaaaa
      781 ttataactat atgatataaa aatatttaat aaatgaaatt tatatgaatt ttttataaat
      841 atattgaaaa aatgcatgca gtatagtaaa ataatactta gctgactaag taatataaaa
      901 aataaggagg gcaagtatga atagcaattc tgaagaaaaa ttaaatgatg ttttcttgca
      961 attaatgcga atatatttta taagaactca tttttatttt gataaattag gcttatatca
     1021 tggtcaaccc caaatacttt tttctttaaa gatgaaagat gggcaaagtc aaaaagaatt
     1081 agcagataaa aggaaagtaa aagcatctac aactacagtt atgataaaga ggatggaaaa
     1141 agctggactt gtggaaagaa aacaagatga aaaggaccaa agagtatcta ggattttttt
     1201 gacagacaaa ggtttagaaa tatgtaataa agttgaagaa tttaacaagg aattggaaag
     1261 agagtttttt ttaggattta gtgaagaaga aaaaatagta cttagaagac ttctaatgca
     1321 agtacgagat aatttaaaag caggatgtca taagcatgaa gatgaaaaat atttttaaag
     1381 tttaaaataa tgtttttcaa tttaatgtta ttagactaat taatattaca gaagggaaga
     1441 aatgtatgtt taaattaatt aaatatttaa agaaatcggc attatcaata gtaataatag
     1501 tctgtctatt ggtaattcaa gcggtatgtg acctttcttt accagaatac acatccaata
     1561 tagtaaatgt aggtatacaa caaggtggag tagaaaactc tgttccatct gtgattagag
     1621 agagtgaatt aaataaaatt actttattta tggataaaag tagtaaggat aaagtactag
     1681 ataattatac tttgctgaat aaaaaggatt atgttaagta taaagataag tacccaggtc
     1741 taaaagatga atctctttat gagttaaata ctaaagacaa agacactata gatgatttaa
     1801 atgttatttt tggtaaagct attttaatag tatcagggct tgagggcgat agtaaagagg
     1861 taaaagctat gaaagctcag ttaatgagca aacttccacc acaagctact caaagtggag
     1921 atgttgatat atttaaattg ttatcagcaa tgccaaagga acagttagat acattgacta
     1981 aagagatgag caaaggcttt gaaagtatgc cagagagtat gataacacaa agctctgttt
     2041 catatgtaag gtcagaatat gagaaaatag gtattgatac agaaaaaact caaaataatt
     2101 atatattatt tactggtgct aaaatgcttg gtatagcaat gattagtatg gtagctacaa
     2161 taacagtagg atttttagca gcaagagtag cagcaagtgt aggtagaaat ttaagaagtg
     2221 gagtatttag aaaagttatg tcattttcaa atactgaaat gaatgaattt tctacagctt
     2281 cattaattac tcgtagtaca aatgatattc aacagataca aatgttgatg gttatgttgc
     2341 ttagaatagt attctatgca cctataatgg ctataggtgg agtgataaag gttttaaaca
     2401 caaatacttc aatggcctgg attatagcag tagcagtagt tgctatctta tcattaattg
     2461 tagtgttatt ctcagtggtt atgcctaagt ttaaactggt tcaaaagctt gttgataagt
     2521 taaacttagt aagccgtgaa ataattactg gtattccagt tatcagagct tttagtactg
     2581 aaaaacatga agaagaaaga tttgataaag caaataaaaa tttaactaaa acaaatcttt
     2641 ttgtaaatag agttatgaca tgtatgatgc ctgctatgat gcttataatg aaccttatca
     2701 cagttcttat agtttggaga ggtgcataca gtgttgattc tggagcaatg caagttggag
     2761 atatgatggc atttatacaa tatacaatgc aaataataat gtcattctta atgatatcaa
     2821 tggtatctat tatgcttcca agagcatccg tttcagctac acgtatagat gaagtattag
     2881 tgtcagatat aagcataaaa gacccagaaa aatcagaggc acaagagttt aaggaagata
     2941 aaaagggtct tgttgaattt aaaaatgttt cgttcatgta tccagacgca gaagaaccta
     3001 tattaacaaa tatatctttt acagcaaagc caggagaaac aactgccttt ataggaagta
     3061 ctggtagtgg taagtcaact cttataaatt taattcctcg tttctttgat gttactgaag
     3121 gagaaatatt acttggtgga gttaatataa aaaatgtttc tcaacatgat ttaagggaaa
     3181 aaattggata tgtacctcaa aagggagtat tgttttctgg tactatagaa tccaacttaa
     3241 aatatggtag aaaagacgca acagatgaag aaatgagaac agcagctgaa atagctcaag
     3301 ctacagaatt tatagattct aaaccggaag cttttaaaac tgaaatttct caaggtggaa
     3361 caaatgtatc tggaggtcaa aaacaaagac tttctattgc aagagcaata gcaaaaaatc
     3421 cagaagttta tatatttgat gatagttttt cagcacttga cttaaaaact gatgcagctc
     3481 ttcgtaaggc tttaaaatca aaaactgctg aaagtacagt tctaatagtt gcacaaagaa
     3541 ttagtactat cttaaatgca gaacagataa ttgttttaaa tgagggcgaa atagttggaa
     3601 taggaacaca taaagagctt ctaaaaaatt gcgaagttta tgagcaaata gctttatcac
     3661 aactttcaaa ggaggagcta gctaatgagt agaatgggaa gaggtcctat gggaaaatct
     3721 atgggagcag gacaaaaagc aaatgacttt aaagggacta tgcgaaaact aattgcatat
     3781 ttatctaaat ttaaaatatc aataattcta gtaattgtat ttgctattgg tagtgcgtcg
     3841 ttttctatag taggtccaaa aattttagga aaggcaacta caaagatatt tgaagggcta
     3901 gtgagtaaag tttctggagg aaacgtaggt atagatttta atgcaattgg aaaaatctta
     3961 acctttttac ttttccttta tttgataagt gcactgttct cttttataca aggatttata
     4021 atgagtggaa tttctcaaaa agtatcctat aatttaagaa aagagatttc agcaaaattg
     4081 gatagattgc caatgaagta ttttgatact aagacgcatg gtgaaatatt atcgagaatt
     4141 acgaatgata tagatacttt aaatcaaagt ttaaatcaaa gtatgacaca gcttataaca
     4201 tcagtaacaa ctatgatagg tgttttgata atgatgttat ctataagtgg aattatgaca
     4261 ttagtggcag ttttaatatt accaatatca atgtttgtaa tttcaagaat aataaagaaa
     4321 tcacaaaaat actttagata tcaacaagaa tatttaggaa atgtaaatgg tcaagttgag
     4381 gaaacttaca gtggccaaac aatagtaaaa gcatttaatc gtgaggatga agtaattgaa
     4441 gagtttgata aactaaatga tagcctgtat aattcagcgt ggaaatctca atttttatca
     4501 ggtataatgc aaccacttat gatgtttatt ggaaacttgg gatatgtaat ggtatcaatc
     4561 ttaggaggtt ggcttgcaat taagaaaaca attgaggttg gtgacataca gtcatttata
     4621 caatatgtaa gaaactttac acagccaatg actcagattg cacaggttgc caatttattg
     4681 caatcaacag cagcagcttc tgaaagagta tttgagtttt tagaagagga agaagaagtc
     4741 caaattgttg aaaatgcagt ttcaatagat ggtttagaag gtaaaattga ttttgaaaat
     4801 gtcaactttg gatataaccc aaacaagact ataataaatg atttctcagt aaatgttaaa
     4861 cctggtcaaa aagttgctat tgttggacct acaggagcag gtaaaactac aatagttaaa
     4921 ctgttaatga gattttatga tgttaatagc ggttcaatac tgattgatgg tcataatatt
     4981 aaagatttta atcgtagtga gcttagagaa atgtttggta tggttcttca agatacatgg
     5041 ttatttagtg gttcaattat ggaaaatata agatatggta agttgaatgc tacagatgaa
     5101 gaagttatag aggctgctaa atctgcacat gttcatagat ttattaagac attaccagat
     5161 ggatataaga tgaaattaaa tgaagaagca agtaatgttt ctcaaggtca aaaacaatta
     5221 ttgactatag ctcgtgctat tttagctgat cctaaaattt taatattaga tgaagctaca
     5281 agttctgtcg atactagaac agaagtattg attcaaaaag ctatggataa tcttatggaa
     5341 ggtagaacaa gttttgttat agcccacaga ttatcaacta tacgtgatgc tgatatgatt
     5401 cttgtaatga atgaaggaga tatagttgaa caaggaaatc atgaggaact tctaaagaaa
     5461 ggcggattct atgccaatct ttacaattct caatttgaag aagatgaagc aatgtaacaa
     5521 aaaactatct tagcatagac atgtaattaa taagataaaa taaatgaggg tgccttaaaa
     5581 tgaacttttt aattcataag gcactctttt gtgtatgaaa taaagtgtat ttttatcatt
     5641 ttaacaatga aaaagaggat tctctatatt ttgagatagc cacataattt tatgtcttta
     5701 caaagtgttt attttattat aatatgtagt aaggaaaatg ttggaggtga ttatcaaaat
     5761 gtatatttaa ttttattctt taaattttat tataaataga atttaatttt aatagttgat
     5821 gattatattg aagtatatgt ttttattact tttattaatc atgcaaataa ctacttaaat
     5881 ttaagaatag ggttaaatat aaaatgataa ttacatataa gttaattaaa ctaatgtatt
     5941 tttaatatat atttagaatt ttaatccttc ttatgatata agtagcttgt tttgtattac
     6001 ttagatttgt aaggagtttt tttattgaaa aaactgcata atatatttgg gaggaggtgt
     6061 aagaaagtga ttttttatat aagattgaga aaataatgca cgattatggt tattataata
     6121 gtaaataaga agtgtcattt aaggataaga taaaaagcaa aattcttgat gaatttaatt
     6181 atagagccat aatctcgtgc tatagattaa ataaaaaata gcagataagg agaatatgga
     6241 tatggcgaaa aaaggaataa cagtaggatt aaacataaat aataaatctg aggactttaa
     6301 tgaactaatg attgaacttg aaaacttatg ttcagcatgt gatattgatg ttgtagggag
     6361 tataactcaa aatgcaaagc aggttaatag agcattttac attgggacag gtaaagttga
     6421 ggaaatatta aacttaataa agaaagaaaa tatagagata gttatatttt acaatgaact
     6481 ttctacatca caactaaaaa atcttgaaga aaagcttaat tgtgaaatta tagatagaac
     6541 ggcattgata cttgatatat ttgcacaaag ggcaaaaact agagaagcta aacttcaagt
     6601 agaattagca agtttaaaat atatgttacc aagacttata ggttcaaatg aaaatttagg
     6661 acgtcaaagt ggtggagttg gtactaaaaa tagaggttct ggtgagaaaa aattagaact
     6721 tgaccgtaga agaatagaag aaaagataac atctttaaat agggagttgg atgatttaaa
     6781 attccaaaga gaaactcaaa gaagtatgag aagaaaatca aatcttccaa atgtagctct
     6841 agttgggtat acaaatgctg gtaaatcttc aataatgaat aaactagtag atatatttaa
     6901 aaactctgaa gaaaagaaag tgtttgagaa aaacatgtta tttgctacac ttgaaacttc
     6961 tgtcagaaat atagttcttg caaataacaa agaattttta ttatctgata ctgttggttt
     7021 tgtaagtaat ctaccacatg atttagtaaa agcatttagg tcaacattag aagaagcatg
     7081 tgaggcagac gtactgttac atgttattga tatttcaaat ccaagctata aaagtcatat
     7141 taaagtgaca gaagatactt taaaacaaat aggtgcagat ggtataccta tgatacatgt
     7201 gtataataaa attgatttaa tagatgtaga agtattggat agaattttag atagtatcga
     7261 taaagaaggt atatttgttt cagttaaaaa agatataaat atagataaaa tgataaaatg
     7321 tatttgtgat agcatattta aagattatgt aagatataag ttcttaattc catatgataa
     7381 aggtcatgta gtttcttatt ttaacgaaaa tacgagcatt ataaatacag aatacagaga
     7441 agatggagct attttagatg tagaatgtag caatatagaa tataataagt ataaaaagta
     7501 tgctttagag taaattttga aaaacttatt atagcctaaa atatatttaa tttataattt
     7561 attataaatt gattgtaaat atgattttat gaaacggtat agccataatg ctatgccgtt
     7621 ttgccattta taataaaaat aaatttattg acataatagt aactaattac tataatataa
     7681 agtaattagt tactatttaa aattatcaaa aaattaaaat ctaagataaa gagaagtgta
     7741 taataatgga tgcagaaagt ttaatgaaaa aatatatggt agatgatgat agtagaaaac
     7801 aagctatatt tgcaagtata tttgtaatgc aaaataaact tcaaactaca tgtgataaat
     7861 tagacccaga attaacaatg aaacaatggt tacttttagc tattgcggac tctgtagatg
     7921 aaactcttac attaaccaaa cttggagaat taatggggtg ttcaagacaa aatgtaaaaa
     7981 aactagctat tgctttagaa aagaaaggtt ttatagaaat tgagcaaaat gaaaaagatt
     8041 caagagctat ctgtcttgtg acaaaagata gagtgcaaga gtattctaaa aaagtgagag
     8101 gtatgcagga aaaagtatta cagcttttat ttgaagattt tacagagaaa gaagcggaac
     8161 agttattttt tggaattatg aaattaagta taggaattga aaatgtgaaa aattatgtag
     8221 aaaataatga cgttggagaa taagcaagta tcaaatcaac tttgtatgac atcagagagg
     8281 ggagaattta tatgaaaagt gtagtagtga tttataagag taagtatggt tcaacaaaaa
     8341 aatatgctaa atggctttca gaattattgt cttgtgatat ttttgaaact aagaatatta
     8401 atatagaaaa ggttatgaag tatgatacga ttattttagg aggaggtctt tatgcttgta
     8461 atatagcggg aatttctttc ttaaaaaaaa attataaaga attgaagaaa aaaaatttag
     8521 tagtatttgg cgtaggagct gctccatatg atgatgaagt aataaagaaa ttaagagaag
     8581 taaactttaa ggatgaatta aacaaaattc cttgctttta ttgtcgtggg gcatgggatg
     8641 agacaaaaat gtcttttata gattatactc tttgtagttt ttttaaaaaa tctatagcta
     8701 aaaaagaacg tcaaggtctt aaaccagcag aagcaccttt attgtctgga gaagcctttg
     8761 attggtgtaa taaagaaaat ttaactccaa tacttgatta tataagagaa ctttaagagg
     8821 tatatatttg taattattta ttatctaaag ttagatttca tagaaattta ctagacatta
     8881 tttataaaat aatattatta aagatggata atttaattgt ttcaataagg ttataatttt
     8941 aaaaaatgtt tattttaaaa taaattattt tataagctta cttatactta ataaggggct
     9001 ttttttattg gaagatttat gttatattga gatgaattta ttatgatata aaaaataatg
     9061 ttaggataaa tttaaatctt tttctaaaaa tgtattaata taattcttaa agaaaggatt
     9121 tttaataatg gatataaaag agttagttaa aatgcaaaga aaatattata atacaggtaa
     9181 aacaagagat atatcgtttc gtatagaaca gttgaagaaa cttaagttag ttgtatcaca
     9241 aaatgaagaa aaaatacttt tagcactaaa gaaagattta aataaatcgg attttgaagg
     9301 ttttatgaca gaagtaggca tgttttatag tgaattaaac tttgctataa agaatataag
     9361 aaaatggagc aaaataaaaa gagttaaatc aagtatggtt aattttccat ctatatccaa
     9421 agttgttcca caaccttatg gtgttactct cataatgtca ccttggaatt atccatttca
     9481 attagcactt ataccattag tttggtcatt agcagcaggg aattgtgtta tattaaaacc
     9541 ttctgaatat tctgtgtcaa catctagtgt agttaaagac atagtggaag atactttttc
     9601 aaaagaatat gttgcagttg ttcaaggaag tcaagaagaa agtgaaaagt tactattgga
     9661 aagatttgat tatatatttt ttacaggaag tactaatgtt ggtaaaataa tcatgaaatc
     9721 agcatcagag catttaactc ctattacttt agaacttgga ggaaaaagtc cttgtatcat
     9781 tttaaaagat gctaatatag accttactgc caaaagactt acttggggaa aactaataaa
     9841 tgcaggacaa acctgcgtgg ctccagatta tgttttagtg catgaagata gaaaaaatga
     9901 gttaatagag aaaataaaat attatactaa taagtatttt ggagataatc catgtaataa
     9961 tgagcaattt cctaaaatca taaatcagag acattttaat cgaatattat ctcttattga
    10021 taaagataag atagtctatg gtggaaatta taataaggag acattaaaga tagagccaac
    10081 tatagtagac aatgttaatt gggatgataa tataatgaag gaagaaatat ttgggcctat
    10141 atttcccatt ttaacatata aagatttgga tgaagtaata caaaaaatta tacaaatgcc
    10201 aaatccattg gcactttata tttttacaaa aaataagtac ctagaaaata aacttttaga
    10261 aatgatttca gcaggaggat gttgtatcaa tgatacagtt actcatatag ctacgaatta
    10321 cttacctttt ggtggtattg gtgaaagtgg tatgggctca tatcatggaa aagctggatt
    10381 cgatacattt actcactata agagtgtgtt aaaaaaatta aatttggatg tccctattag
    10441 atatgctcca tacgataata aattaattaa ggttcttaaa aagataatgt aggttatatt
    10501 gtagaataat aaatataaat aaaattgagt atttgtattc tgaatatatt ttatttttaa
    10561 tttggtatat ttgttataaa agtggtgatt ttgtgataaa atttaattat atagaaataa
    10621 ggaggaaaaa atgaaaatta attggaatac aaaatacaca acaatagcaa tttatacatt
    10681 tataatagct gctagtagta ttatatttta tctagtatca tcacaaattg atgtattttc
    10741 taataattta gatgctatat ttacaacatt acaacctttt ataataggat ttgctattgc
    10801 atatcttctt aattttattt taaagtttta tgaagacaga atttttataa aaagtgagaa
    10861 attaaaaaaa ctaaaacaaa gttcaaaaag aggcttgggt cttttattaa catatgcaac
    10921 agctacattg atactttact tatttatgca tttcgtattg ccacaagtaa ttgagagtat
    10981 tgttggacta gcaaatgata tacctatgta tgtaaacaat gcaactaagc ttattgataa
    11041 attaatgact gatttaaatc ttgatgagca atactttaat ttagcagttg ataagtggaa
    11101 tgagtttgtt acatatatta taaagtttgt aacagattta atcccaatat taggaagtat
    11161 gttaaagaat gtagcctcta gtatatggaa tattgtacta ggattaattg tttcagtata
    11221 cttattaatt gataaagaaa aattctatgg attaagcaaa aaaataactt atgcagtatt
    11281 tacagaaaaa caagctgcta gaattttaga attaactcat agaagtaact atacatttgg
    11341 aagattttta ggtggcaaaa tattagattc attcattata ggtattctaa catttgtaat
    11401 acttacatta gttaaaatgc catatacatt attaatatca gttataattg gtattacaaa
    11461 tattatccct ttctttggac ctttatttgg agctatacca tccacgctaa tagtgatgtt
    11521 tgtatctcct ataaaagctt tctggttatt gttaattata ttaataatac aacagataga
    11581 tggaaacatt ataggaccta aaatacttgg agattcaatt ggtatatctg cattttggat
    11641 tttgttctcc ttattggttg caggaaaatt attaggtttt attggaatgg ttataggagt
    11701 tccaatgttt gcagtaattt attctataat taaggatatt gttgaaagta aattggacaa
    11761 aaagggatta ccgacagata cttccgatta tatgtagcta aaaaatatta tgtataatta
    11821 aaaatatata aattaagcaa tatttaaagc actgagaaag ttaaactctt ggtgcttttt
    11881 agatataaaa ttattataga agaggtgttt tttgtggcat ttatagaatg taaatttaaa
    11941 tctaaatata ctggaggaga aactgatata attgtaattt tgcctgtagc aaatggaaaa
    12001 gaacttttta gtggtaaaga gatatatgat tttgaaggta aatttaaaac attatactta
    12061 tttcatggtt tagaagatga ccaaagctca tggattagat atactaatgt agagcattat
    12121 gctaaaaata aaaatatagc cctagtaata ccaagagtag aaactagttt ttataccgat
    12181 atggcgtatg gacaccaata ttttactttt gtaagcgagg aattacctaa atttgttaga
    12241 gctatatttc ctttatcaga taaaagggaa gataactttg tagctggtat gtctatgggg
    12301 ggatatggag ctttaaaatt agcgctttca aaaccaaaag agttttcggc agttgcaagt
    12361 ttttctggtt cgcctgatat gattcatgag ttagaaaatc aacaaataga aaccaatgca
    12421 gacattctat ttcacgattt tggtagacca gaagacgcta gaaatagtga gaatgattta
    12481 ctttacatac ttaagaacct taaagaaaga aatgaagata ttcctaaaat ttatcaattt
    12541 tgtggtgata gagatttttt atataaaaat aatcagatgt ttaaaaaata tgcagattct
    12601 ctgggtgtag atgttgaata tgaagagtgt gtaggggggc atgaatggcg tctttggaat
    12661 ttatggataa agaaatttat agatataatt ggatagtaca cttatggtat taacaggtat
    12721 actataaaat atctttaaat atgataaaga ctgaaatcct aaaatcaaat tatgatttta
    12781 aaatttcagt ctctaagaat ttaataaaat tttattaagt tcttacacga gttattattg
    12841 gagtaaaatt gtcaactata tctatatagt atttactcaa ttcctctaaa atctcttcgc
    12901 ataaatccgt actatgacca agattaataa gaaactgttt tgtaaaatgc tttattacag
    12961 cttgagaaga tatatcgtac ttgtgtgaag tataaactct aattatttca ggtttcatag
    13021 caagtatacc aatacaagaa agtattaatt catcatgttc tatagattct ttatcattaa
    13081 catatctttt catgaccatt tcggttacac ttctttgata gcttaaaata gcttcttcat
    13141 aagctatatc caaataaaaa tttctatatg cttcgttttt tagaaggaaa caaaggtcag
    13201 cattcatagc aagaaagaac atcatcatag taccttcttc atggtagtta tagattaaag
    13261 actgcatttc atttcttata tcttcatata ttatagctcc tatttcgctt ttacttgaga
    13321 agtaatagct tattagacct aaatttatat tagctctttc tgcaatttgc ctagttgttg
    13381 tcttatgata accttgctca aaaaataatt ctctagcagc acttataata ctagcttttg
    13441 tttctttgcc cttttctgat tttgacatat ttactgtgta tacctctctt ttcaatcttt
    13501 ttttgaaaat ttgctaacta taaatattgt aatagaacca cctattatag gaactaaaat
    13561 tctgtcaaat acagtaagaa taccaatcat atttaataat gccaatataa cacttactat
    13621 aacagcatag atagctcctt tttttgtagc acctttccag caaagaccaa ttacaaatgg
    13681 tgcaaatatt acacctgtac ataatggagc tggccaaaac aataattcta ataaactatc
    13741 aaatttaagt gctatgattg cacctattat agctgtcaaa gctgatgcaa tttgagaata
    13801 ctttagagta ttttttaaat cattaaattc tatattagga tgtaataatt tgttataaat
    13861 atctcttgat ataatagcac tcattgaggt cattaatgaa tcggcagtag acattatggc
    13921 agccaatata gcagctaaga ataatacaga tattaaaggt ggcaaagtag tcgatatcat
    13981 ttgaggtaaa acctgaacag atgatatatt tggaaataaa ctaccaccaa tagctccaat
    14041 tattcctata gggaaagcaa gaaataatag tataaatcct gcaattacac tacttttaac
    14101 tgcattttta gcattatcag cacattgata tcttaaaaaa ctttcataag aaaatattga
    14161 gcctaaaact aatggtaaaa ctgtatacat aaaatcacta atcgatttat ttcctggaaa
    14221 gaatggttct tttaccattg attgtaacat aagtaaaggg ttgtcgccaa ctaattttaa
    14281 gcttatataa gctactattg gcaaaaacac tatacaaata gcaagttgaa taaatgctga
    14341 tactgcaact ccccacattc ctgatgtata ggtatatata attataacta taactcctat
    14401 aattacagct atatcataag gaataccaaa tgcagtagat acagaaccaa aagaaattaa
    14461 ttgactaccc ataatagcta gtatggatat tggtgttaca agactttgat aagctcttaa
    14521 aaatacacta tgaccatatc tagtttcaat ataatcagca ggtgttatat cacaagataa
    14581 gcttcttaat tttcctgcga aaaatgcatt tataaagcaa gctaaagctg cacctatagt
    14641 agaatacatt ccaacccaaa gtcctctttc agctccccat tgaactccac caactaagca
    14701 ccctcctcca aaagatgtag cagcaatggt tgcagctagt ataggcaaag gcagatttct
    14761 acctgcgatt aggaaatcgg atgctgtttt aacttttttt gaaaaataaa aaccaatcca
    14821 tatgtatatt gcacacgtaa caattaaaag taacatagtc ttttctcctt tataaaatat
    14881 tttaataaaa tatacaaaat atatttaaaa tatattttat ataaaagtag tacgatattt
    14941 gttttattgg ttttagatta atatagctat aataatttat gatattagat ggaaattacg
    15001 taaaaataga aaattgaaaa ttctgaaatt tttgcatatt gatatttatc tcatagattc
    15061 ttttttgtaa ttgtaacacg cttttatttt taaatcaata gtacatgtat aaaaataaaa
    15121 ggaatttaaa taag
//
LOCUS       DAELLV010000080        14998 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.080, whole genome shotgun sequence.
ACCESSION   DAELLV010000080 DAELLV010000000
VERSION     DAELLV010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14998
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.080"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            156..1784
                     /gene="aspD"
                     /locus_tag="KPD58_001919"
     CDS             156..1784
                     /gene="aspD"
                     /locus_tag="KPD58_001919"
                     /EC_number="4.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 4-decarboxylase"
                     /protein_id="HBG0265688.1"
                     /translation="MKNHTYKEIKNIYGKISPFEFKDKLIDIAKYTAKENNRELLDAG
                     RGNPNWTCSTAREAFFTFGHFAITETRSNWDLGHLAGMPQKKGIKERFFKFINENIDM
                     PGAYLARDIINFGINELGFDGDEFVHELADGIIGDNYPLPDRMLPHMEKIVHDYLVQE
                     MKYDISGKYGDVEIFAVEGATAAMCYIFDSLMANELLKKGDTIALMTPIFTPYLEIPN
                     LPRYDFKVVNINANEVDEKGAHTWQYTKKELEKLRDKSIKALFVVNPNNPASIAMDET
                     SCNNLIDVIENYNKDLMIISDDVYGTFVEDFSSLMSKLPYNTVGVYSYSKYFGVTGWR
                     LGTFALHKKNVFDKKINDLTGELKKSVDKRYSDMSLNPSSLSFMERVVADSRLVALNH
                     TAGLSTPQQVQMAFFSAFALIDKVDAYKKLNMSICHRRQKLLFEALELPINENKNNAA
                     YYTQFDIEEWAKLNYGEDLFKFISKTASPVDVLYKLANDYSVVLLSGNGFYGPEWSIR
                     ISLANLYDEAYTKIGKAIREILNGYIVEWQKIKK"
     gene            1905..2900
                     /locus_tag="KPD58_001920"
     CDS             1905..2900
                     /locus_tag="KPD58_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBG0265689.1"
                     /translation="MSIKKKVAIVFTGGTISMTVDDKVGAAIPTLSGEQILSMVTNID
                     KVADVEVFNFDEIPGPHITPYRMMELKNYVNDLLARKDITGVVITHGTDSLEETAYFL
                     DLTIDNIKPVIVTGAMRSSSELGYDGSSNLSASVCTAVSKDAMGKGVLVVLNNEVLLA
                     SEATKTNTLSLNTFKSLTSGPLGIIDCNELVLTRDIVNRTIIDTDKVESKVALFKAYV
                     GENADFIRFAVDSGYKGIVIEAMGRGNIPPQMLSGVEYAREKGLPVVIVSRCHSGRVF
                     DSYGYFGSGRDLKNLGCIFGGDLPGQKARIKLILALGKTDNLDEIKDFFEKGIYC"
     gene            complement(3078..3461)
                     /locus_tag="KPD58_001921"
                     /pseudo
     CDS             complement(3078..3461)
                     /locus_tag="KPD58_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     regulatory      3688..3785
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     repeat_region   3924..4087
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3925..3955
                     /rpt_unit_seq="caatttacattccatatagttaatataaaac"
     gene            complement(4373..4531)
                     /locus_tag="KPD58_001922"
     CDS             complement(4373..4531)
                     /locus_tag="KPD58_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265690.1"
                     /translation="MDNFLQGILASLSASLIVYIASKLFRKRKKPLKAATKSGWEFDL
                     KIRFHKTK"
     gene            complement(4825..6195)
                     /locus_tag="KPD58_001923"
     CDS             complement(4825..6195)
                     /locus_tag="KPD58_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp29"
                     /protein_id="HBG0265691.1"
                     /translation="MKLKKLLSLGLVSMFVAGFPISANALDKIDKIQGADKYETAGII
                     ADKQSYTTAILINADSTMADGLSASGLAGATNAPILLTKKNNIPNATLKRVEKAKKVY
                     IIGGESSIDKATETFLKDKGIETRRLQGNDRIKTSYNVAKEINSINKVNKVILTNAFK
                     GEPDAMSAAPVAVRDKAAIVLTDGKSVPFNTTGIESYAIGGTSSMSDSLVNDTNSTRL
                     GGVDRYDTNKKIVNKFYNGAKEFYIASGTDLVYALVASPMAKNNPIVLVDIGSNKDIL
                     KNAAKVTGIGNLSDKIMLECENTINNISLPSSNKNIICGLKIDFDSNHIIWQDKGSYL
                     ECYDDNLEYYTEKYKDPTVPINIENTSTQNLDVTKLKVYGEANSTLYMGQYAKIKMDF
                     NSFGVLKPGEKCTIYLDIISLDPSRSMTLTFSYVGDKENYKYEQGLASRILFYNPDGE
                     DFFEYK"
     gene            6411..7528
                     /locus_tag="KPD58_001924"
                     /pseudo
     CDS             6411..7528
                     /locus_tag="KPD58_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861579.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(7891..7971)
                     /locus_tag="KPD58_001925"
                     /pseudo
     CDS             complement(7891..7971)
                     /locus_tag="KPD58_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005541856.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(8011..8679)
                     /locus_tag="KPD58_001926"
     CDS             complement(8011..8679)
                     /locus_tag="KPD58_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBG0265692.1"
                     /translation="METVTNNQSGVEIQLSLFEAIKDLLLINPIFLLGIILIGIFLVV
                     IFKKNSDIPKVKTSILSLAMYYYLCLTLSHIVGIPTFSEYIRLSQLGETFFNPNINLI
                     PFSDGFSLSFILNILLFVPLGFLCPLISKTFERLRNTFLIGFGLSLFIETIQLFTLHR
                     ATDIDDLLTNVIGTIIGYFCFKLIAKLRIVKLHSDDDSMEQDYTVCIPIAIIIVALIL
                     GFFS"
     gene            complement(9532..11952)
                     /locus_tag="KPD58_001927"
     CDS             complement(9532..11952)
                     /locus_tag="KPD58_001927"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBG0265693.1"
                     /translation="MSVYNFKEVESKWQKIWKDNNQYKMDTAQTEKPNYYTLEMFPYP
                     SGKIHMGHVRNYSIGDVVARFKKMEGYNVLHPMGWDSFGLPAENAAIKHGIHPHKWTM
                     ENIEEMKEQLNLLGISYDWDKEVATSTPEYYRFTQEIFLKFLEHGLAYKKKSYVNWCP
                     SCETVLANEQVVQGACERCKATVLKKDLEQWYFKTTEFAEELLNDLDILDGWPEKVKT
                     MQKNWIGKSTGADLVFDIDGTDKSMTVFTTRPDTTYGVTYMVLAPEHELVKELVAGTE
                     YEADVEAFVQKMHTMTEIERTAADVEKEGMFIGRYVINPLNGKKVPLWIANYVLVEYG
                     TGAIMAVPAHDERDRDFAEKYNLDIIDVITEDNKMINSEEFNGLDASEGFEGIIDKLE
                     KEGRGKRTINYRLRDWLVSRQRYWGCPIPVVYCDECGIVPVKKEDLPVLLPTDVEFTG
                     KGESPLTTSKQFMSTTCPHCGKPARREVDTMDTFVDSSWYFLRYVDSNNENEPFSKEL
                     VNRWHPVDQYIGGVEHAIMHLLYARWFVKAFKSMGMVDFNEPFKNLLTQGMVLMDGSK
                     MSKSKGNTVSPMDIIDEYGADTARLFVLFAAPPERDLDWSEQGVDGCFRFLNRVYRLV
                     DELADVVKKDVEFGELNSQDKDMRYTIHSTLKKVTADLSEKFGFNTAISALMELINDM
                     YKYKELDNINEAVIKEGVQTIVTIIAPFAPHLGEELWTMIGKEGSVFDIDWPKYDEKA
                     LVKDEIEVVVQVNGKVRGKLTVNSNISKDEMEKVALEDEKIKGLVEGKTIVKVVAVPK
                     KLVNIVVK"
     gene            complement(12306..12656)
                     /gene="rsfS"
                     /locus_tag="KPD58_001928"
     CDS             complement(12306..12656)
                     /gene="rsfS"
                     /locus_tag="KPD58_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBG0265694.1"
                     /translation="MTVEQMTKIAYDAIEDKLGQDTVIINIGKVSSLCDYFIITTASS
                     QRQVKAIADNVEDELAKLGLEPRGKEGQGTQTWVLLDYGDIMVHVFNEENRGFYNLEK
                     LWKDAPYIDIDTLA"
     gene            complement(12773..13345)
                     /gene="yqeK"
                     /locus_tag="KPD58_001929"
     CDS             complement(12773..13345)
                     /gene="yqeK"
                     /locus_tag="KPD58_001929"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HBG0265695.1"
                     /translation="MNLENINQRLNQMLPVGRLSHSKNVAKCAEKLCEIYGCDKEKAY
                     LAGMIHDCAKYLSDKEIEDYVNKYEIYLDPIEDGNRSLSHSVIGAYICEYEFEVEDED
                     IINAIKYHTTGREDMSLLEKIIYIADLIEEGRKFPVVDTLRELAYGGKLDEALLTSFN
                     NTLMFVINKKEEIHPRTVMARNYLIKEKLL"
     gene            complement(13346..14035)
                     /locus_tag="KPD58_001930"
     CDS             complement(13346..14035)
                     /locus_tag="KPD58_001930"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBG0265696.1"
                     /translation="MRSENLVKMAELKNTEKLEKFNRHKGKIKIGILGGTFDPIHYAH
                     LATAEFIRDKYDIDKIIFIPSGNPPHKLCITTDKYDRYNMTLLATESNEDFLVSKVEI
                     ERKKRTYTIDTLKYLKKKYKNADIYFITGADAICSVEEWKDVKKNFELATFIAATRPG
                     ISLLRSQETIEKLTKKYNADIITVYVPSLDISSTYIREQLNEGKSIRYLVPENVENYL
                     YENKLYQYGDD"
     gene            14311..14949
                     /gene="recX"
                     /locus_tag="KPD58_001931"
     CDS             14311..14949
                     /gene="recX"
                     /locus_tag="KPD58_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBG0265697.1"
                     /translation="MGIITKIEQQKRNDDRVNIYVDDKFFIAIFKELVYTFNLKKGNE
                     INEEELKPILDDEMYIKAKNKALNILSHADQSEKKLKEKLSSEFDENIIERVIDFLKS
                     YNLVNDSVLAQKIVNTNVNLNKCGKNRIKQNLYNKGINRSTINEAVSELDKDTEFENA
                     MYLAKKRYERVKKEDKKKIYQKISQHLSYKGFDYDIIKRVLNKLLNFDEYDI"
BASE COUNT         5137 a         2383 c         1911 g         5567 t
ORIGIN      
        1 tttaacatat atttaataag gctaaataga gttattattt taatttcata ctttactttt
       61 tctataaacc tattataatt aaagtataat catttttgca acttgtttat tttattataa
      121 aaatgtgaaa cttattacat aaggaggaat ttaacatgaa aaatcatact tataaagaaa
      181 tcaaaaacat atatggaaaa ataagccctt ttgaatttaa ggacaaatta atagatattg
      241 caaaatacac tgcaaaagaa aataacagag aattacttga tgcaggtcgt ggaaatccaa
      301 actggacttg ttcaactgct agagaagcct tttttacttt tggtcatttc gctattactg
      361 aaactcgttc taattgggat ttaggtcatt tggctggaat gcctcaaaaa aagggtatta
      421 aagaaagatt ttttaaattt ataaatgaaa atatagatat gcctggagca tatttagcta
      481 gagatattat aaactttggt ataaatgaac ttggatttga tggagatgaa tttgttcatg
      541 aactagctga tggaattatt ggtgataact atccacttcc agatagaatg ttacctcata
      601 tggaaaaaat agtacacgat tacctagttc aagaaatgaa atatgatata tcaggtaaat
      661 atggtgatgt agaaattttt gctgttgaag gtgctacagc tgctatgtgc tatatatttg
      721 attcattaat ggcaaatgaa cttctaaaaa aaggagatac catagcctta atgactccaa
      781 tatttacacc ttatcttgaa atacctaacc ttcctcgtta cgactttaaa gtggtaaata
      841 taaatgctaa tgaagttgat gaaaaaggtg ctcatacatg gcaatataca aaaaaagaat
      901 tagagaaact tcgtgacaaa tctattaaag ctttattcgt tgttaatcct aataatcctg
      961 cttctatagc tatggatgaa acatcttgta ataatctaat agatgtaatt gaaaattata
     1021 ataaagattt aatgataata tcagatgatg tatatggaac ttttgtagaa gatttttcat
     1081 ccttaatgtc aaaactacct tacaacactg ttggagtata ttcttattca aaatattttg
     1141 gagttacagg atggagactt ggtacatttg cccttcataa gaaaaatgtt tttgataaga
     1201 aaataaatga tttaactgga gaattaaaaa aatcagtaga taagcgttac agtgatatga
     1261 gtcttaaccc ttctagcctt tcatttatgg aaagagttgt tgctgatagc agacttgttg
     1321 ctctcaacca tactgctggt ctttctactc cacagcaagt acaaatggcg tttttctctg
     1381 cttttgcatt gattgacaaa gtagatgcct acaaaaaact caatatgagt atctgccata
     1441 gacgtcaaaa actattattt gaagctttag agcttccaat taatgaaaac aaaaacaatg
     1501 ctgcatatta cactcaattt gatattgaag aatgggctaa attaaactac ggagaagact
     1561 tatttaaatt tataagtaaa actgcatctc cagtagatgt tctttataaa ttagctaatg
     1621 actattcagt agttttactt agtggaaatg gattctatgg tcctgaatgg tcaattagaa
     1681 tatctctagc aaatctatat gatgaagctt atactaagat aggaaaagcc ataagagaga
     1741 tattaaatgg ttatatcgtt gaatggcaaa aaattaagaa ataattatat tacactgaaa
     1801 tatctaaatt atatgaataa aagagtttat gtagacctta actacttttt ttgatataat
     1861 cctatcggaa ttataaattt tatttatgaa aggcagagaa tagtatgagt attaagaaaa
     1921 aagttgccat agtttttact ggtggaacta tatctatgac agtagatgat aaagtcggag
     1981 cagctatacc aactttatca ggtgaacaaa tactatcaat ggttaccaac attgataaag
     2041 ttgcagatgt tgaagttttt aattttgatg aaattccagg acctcacata acaccttata
     2101 gaatgatgga attgaaaaat tatgtaaatg atttattagc aagaaaagat attacaggtg
     2161 ttgtaataac acatggtaca gatagtttag aggaaacagc ttattttcta gaccttacaa
     2221 ttgacaacat caaacctgta atagtcacag gtgctatgag aagtagttct gaacttggat
     2281 atgatggttc aagtaattta tctgcatcag tttgtactgc agtatccaaa gatgctatgg
     2341 gaaaaggagt ccttgtagtt ttaaacaatg aagtattatt agcttctgaa gctactaaaa
     2401 caaatactct ttctctaaat acatttaaat ctctaactag tggcccatta ggaataattg
     2461 actgcaacga acttgtcctt acaagagata ttgtaaatag aacaattata gatactgata
     2521 aggtagaatc taaagttgcc ttatttaaag catatgtagg agaaaatgct gactttataa
     2581 gatttgctgt tgatagtggt tataaaggta tagttattga ggcaatgggt agaggtaata
     2641 ttcctcctca aatgctttct ggagttgaat atgctagaga aaaaggactt cctgtagtta
     2701 tagtttctag atgtcattca ggtagagtat tcgatagtta tggttacttt ggttctggta
     2761 gagatttaaa aaatcttggt tgtatatttg gtggggactt accaggtcaa aaagctagaa
     2821 taaaacttat cttagcactt ggaaaaactg ataaccttga tgaaatcaaa gatttctttg
     2881 aaaaaggaat ctattgttaa ttataataac ttctatttaa ttatttattg atatttcaac
     2941 atttttaaat caaaatctaa tacttatgtc agttaaatgt cagcaaaata ttttatccac
     3001 aagttttcca caacttatcc ccaatattat ccacaagaaa aaaggtagca cttatattag
     3061 tagctacctt ttatttatta atcttcatct ttccagcttt ttatccaatt tcctatttct
     3121 attatttttt cttcagttat ttcttctcca tcatgcaatt ttgaaattaa ttctgataat
     3181 ggattgttat gtagacctcc aaaaattctt tttccctcta ttttgagata ttctttctct
     3241 tttattaata ctctatagtg tacatgtttc ccaattctct gcgatatgaa aaaacctttt
     3301 ttagttaatc tagctaaaag tgtaattgta gttgtatgct tccaagcata tgttttttct
     3361 atttcatctg caacatcttt tgatattaca gttttataac cagtattcta tatgtatttc
     3421 ataattttta attctgcatc tggtaatttt ttattcaaca taatatattc cttctttcaa
     3481 tttatgtcac ttcctcaaac ttatctagct taaattaata ataggagctt tttatcacaa
     3541 aaactacagg taaatttttc tatatattct acgtatttag tctgtttttt aagtgtgaaa
     3601 ttacgttcat acctataaat ttattcaatt ttcaaacaat cttattgaat aataactaat
     3661 tctattgtat tatttagtta tagaataaaa ctaaatcggc aaaactagag aaatctagtg
     3721 acgcaaagct atagagacta agacttatat aaatatctta tgagttatgt cagccagttg
     3781 ccaaagagat attgttcttt ttgtttttat gaaagttttt tagggggata ataatttata
     3841 tatttagaga taaaatagat aaatgtacac atatattgac tgcttatatt agcagttcgt
     3901 atgattattg taattttttg gatacaattt acattccata tagttaatat aaaacaagtg
     3961 tttcgaattt cttttctgaa actataccaa caatttacat tccatatagt taatataaaa
     4021 cgttatatac tgaaagcggt gaaactggtt acgtagcaat ttacattcca tatagttaat
     4081 ataaaacccc aaaataaatt gagtatttcc aatgcctaca catatacaaa tctctcaaat
     4141 ttgcagtgaa ccatgagtag tgcaattgat aacacttatc acaagtcctc aatgccttat
     4201 atttcaactg ttaagcctta atttcttcaa aaaatcgaac actgcaaaat ttctatgact
     4261 ttattatatc ataaaaatat tatttttgaa tatctgtacc aatttgtgat ataataaaag
     4321 caagaagaac tacaatctat tttgctgtag agtggagttc ataatttaga aattacttag
     4381 ttttatggaa tctgattttt aaatcaaatt cccaaccact cttagttgcc gctttgagtg
     4441 gttttttacg ctttctaaat agtttgctag ctatataaac tattaagcta gcagatagac
     4501 ttgctagtat gccttgtaaa aaattatcca tgtatattca cctcccttct taacgtcggg
     4561 aggataattt tttgatacat gaactccact ctataaattg tagattacat cttcttgcta
     4621 aaaatattat aacatataat tcttacatat tttacctatt tattactttt tacattataa
     4681 taatattctt ttaatcaaaa aaacacctac tattaaagta aatgttttta agtattttta
     4741 attttaaaat ccatatagtt catatgaaac taaatataat aactcaacat atgagcaata
     4801 tcgagacatt tacataccca ctagttattt atattcaaaa aaatcttctc catccgggtt
     4861 ataaaataat attctacttg ccaatccttg ttcatattta taattttctt tatcacctac
     4921 ataagaaaat gttaatgtca tgcttcttga tggatctaat gaaattatat ctaaataaat
     4981 agtacatttt tctcctggct ttaatacacc gaaactatta aaatccattt ttattttagc
     5041 gtattgcccc atatataatg ttgaattagc ttctccataa acttttaatt ttgttacatc
     5101 taaattttgt gtacttgtat tttctatatt tataggaact gttgggtctt tatatttttc
     5161 agtataatat tcaaggttat catcataaca ttcaagataa cttcctttat cttgccaaat
     5221 tatgtgattt gaatcaaaat caatctttaa tccacatatt atatttttat ttgaagatgg
     5281 aagactaata ttattgatgg tattttcaca ctctagcata attttatcac ttagatttcc
     5341 tatcccggtt acttttgcag catttttaag tatatcttta ttacttccaa tatcaactag
     5401 tacaatagga ttatttttag ccattggtga agcaactaaa gcataaacta aatcagtacc
     5461 actagctata taaaattctt ttgcgccatt atagaactta tttactattt ttttattagt
     5521 atcatatcta tctactccac ccaatcgagt tgaattagta tcatttacta aactatcact
     5581 cattgatgaa gtgccaccta ttgcataact ttctatacca gttgtattaa atggaacact
     5641 ttttccatca gttaatacta tagctgcttt atctctaact gctacaggtg cagcactcat
     5701 agcatctggc tcacctttga aagcgttagt taatattact ttatttactt tattaatgga
     5761 atttatttct tttgctacat tataacttgt cttaatccta tcattacctt gaagccttct
     5821 agtctctatt cctttatctt ttagaaaagt ttctgttgct ttatcaatgg aactttctcc
     5881 tcctattatg tatacctttt tagctttttc tactctttta agagttgcat taggtatatt
     5941 attcttttta gtcaaaagga ttggtgcatt tgttgctcct gcaagtccac tcgcacttag
     6001 cccatcagcc attgtactat ctgcatttat aagtattgca gtagtataac tttgtttgtc
     6061 agctatgata ccagcagttt cgtatttatc tgctccttga attttatcaa ttttatcaag
     6121 tgcattagct gatataggaa aaccagcaac aaacattgac actaacccta gtgataataa
     6181 tttctttaat ttcacaatat ctcccccttt tatatatgaa atactatata tcttaataat
     6241 tctacattaa attttctttt cctcctttaa cgatatttat cttcctgtta atttaagtgt
     6301 atacaaatac gtgttataat gtaactgtaa agaattaaaa agataatgac ataattagag
     6361 tagtacaact gttcttttat aaaactaact tataaaggga gcgtacatat atgaatatca
     6421 aatcagcttt tataagaaaa agaggggaaa aattctatgt atatgtggag tacatagagg
     6481 aaacaactgg caagataaaa cagaaaagtt atggaagtta tgaaaagaaa aaggatgctg
     6541 aaaaacattt aattgaaata aaatctacta taaacaataa taagtttgtt gctccaaaag
     6601 atatgacatt tgtagaaaga tgttataaat acatggacat gaataaaaat aattggtctc
     6661 catatactgt tacaaataca aaatcttggg ttaaaaatta catagaacct ctttttaaga
     6721 atctaaaact cacagaagta aatcctagtt cagtacaagc ttttgtaaat tataacttta
     6781 ataattctac ctttactagt gctaaagtaa ggtatagctt tctaagttct gtaataaggg
     6841 aagcatatag attaagagaa atacaagaaa attcatgtga ttttataaaa cttccttcta
     6901 aagaaaaata ttctacaaat gaaatataca atagaaaaga agtattattg ctaatagaaa
     6961 agttacttag taataatata gaaataccta ttcttttaat gttacttcta ggtttaagaa
     7021 tgggagaagc tactgggctt aggtggtcag atgttgactt agataacaat ctgataaatg
     7081 ttaatcaaat tcttgtctat tctaatggca aaatatcttt taaagagcca aaaacaccaa
     7141 aatcaaaaag agctttatca gctccaaaag aattaattga aaaattgaaa attaaaaaac
     7201 taagacaaag caaattaaaa ttgtaaggta tcctggaaaa cagaaataat ttagtttgtc
     7261 taaacaccaa tttaaatccg tttctacctt cttcactaag aaaaacgttt cacaatttta
     7321 caaaaaaaat aatttaaaaa agattagaat acatgattta agatatacaa gtgccactct
     7381 gctgctttta gggggaacta atatgaaagt tgtttctgaa agattgggtc atacagatat
     7441 aaaaataact atgaatagat attctcatgt acttaaagaa atggacaaag aagcttccga
     7501 caatctaagc gaacttctat ttaaatagta aaaactgact gtcagttaaa tgtcagttaa
     7561 acctaaaaaa ataggttttg tcagttgatt atcagtcaat tattttataa tagtttcact
     7621 taaaaacgtc tatatctaca cattttacta tttgtttatg cttattaata tctatctgtt
     7681 attattaatt ctaatagctt ctatttaatt atttagttca gtttacagga aactacacac
     7741 aaaccataaa ataggatgtt gacaatataa cagatagtat gctcctgtaa aaggtaacgt
     7801 actatctgtt atgttgtctg gatttccaaa tggttaccct ttggcacaca actttgctat
     7861 ctccttggta aactaaaagt ttgtaatcta aaatcctaat ccatctctat aaaatttaat
     7921 tgacttctcc atacttttta cacctaaaca tataaaagta attttattca cttaatgacc
     7981 tccataattc ccaaattagc tatttggaac ttaactaaaa aatcctaata taagggcaac
     8041 tattataatg gcaatcggaa tacatactgt gtaatcctgt tccatagagt catcatctga
     8101 atgtagtttt acaattcgca atttagcaat cagtttgaag cagaaatagc ctattattgt
     8161 tccaattaca ttcgtgagta agtcgtctat atcggttgct ctgtgtaacg taaaaagctg
     8221 aatagtttcg ataaaaagag ataatccaaa accaatcaag aatgtatttc tcaaacgttc
     8281 aaaggttttg ctgataagcg gacataaaaa acctaatggg acaaaaagta aaatattcaa
     8341 aataaaactc aaactaaatc catcactaaa aggaataaga tttatgtttg gattaaagaa
     8401 cgtttcccct aactgtgaca gtcttatata ttcactgaat gtagggatac caacaatatg
     8461 cgatagtgtt aaacataagt agtaatacat tgccagtgat aaaatagatg tttttacctt
     8521 tggaatatca ctatttttct tgaatattac aactaagaaa ataccaatca agataattcc
     8581 caacaagaaa ataggattaa ttaacaataa atctttgata gcttcaaaca gactgagttg
     8641 aatttctaca cctgattgat tatttgttac agtctccatt tgtacatacc tcctcataac
     8701 atttcaatta tttctaaaac acaaaataga agataaatct taaaatcatc ctatcaaaat
     8761 ttttaaattt gcctttatca gtttatacca ccagttaatt cctgtcttac ttctgtatgg
     8821 tattcttggg attctttcat caaattagac aaaaaatggt taccatatca ataaactggt
     8881 tacgctccat atctcccgaa gcaaccatcc cctgagaata aatccgtatc aggaatctcc
     8941 acatctactg cacctatcac aagttatttg ataacatcct cagacaatcc caacttggat
     9001 atatcgacaa cacccattag aataccactg ctgaccatac ttgttttcct gctgataatt
     9061 ttaatcaact gggaagttga aacgctatct tttttacaag ttccttttta gaaacatggt
     9121 agctattata tgaatctgaa tttcgttgtc tgggtgtttc atataaagca tagatacgca
     9181 ccttttttgc ctgttttcat tatatagaaa aaaatttcaa aatacaataa ttatattttt
     9241 ttcgtttaag cttagagcag ttccttgatg aacaacactt tggaaactga atgatttaca
     9301 taccacttaa tacattaact cgatttaaca ttaattataa taatatttaa aacaacaact
     9361 agggtatata agaatgaact ttttagttct ttaatcattt ttttgtataa aatcaaatct
     9421 atttttataa tttcaacaaa tttataattt caacaatgaa aaaaggctta tctctatttt
     9481 gagacagcct cgtttatttt ataaatttat atttttgttc atatttttga gttatttaac
     9541 aactatattt actagttttt taggtacagc tacaacttta actatagttt taccttcaac
     9601 taatcctttt attttttcat cttctaaagc taccttttcc atctcatctt tagatatatt
     9661 tgagttaaca gttaattttc ctctaacttt tccattaact tgtacaacaa cttctatttc
     9721 atcttttact aaagcttttt cgtcatattt aggccagtct atgtcaaata cacttccttc
     9781 tttacctatc atagtccata gttcttcacc taaatgaggg gcaaatggtg caattatagt
     9841 tactatagtt tgtacacctt cttttataac tgcttcgttt atattatcta actctttata
     9901 tttatacata tcatttatta attccataag agctgatata gcagtattaa atccgaattt
     9961 ttcacttaaa tcagcagtaa cttttttaag tgtagagtgt atagtatatc tcatatcctt
    10021 atcttgggaa tttaattctc caaactctac atccttttta accacatctg ctaactcgtc
    10081 aaccaatcta taaaccctat ttaagaatct aaagcatcca tcaacacctt gctctgacca
    10141 gtccaaatct ctttctggtg gtgctgcaaa taatacgaaa agtcttgcag tatctgctcc
    10201 atactcatct attatatcca taggagatac tgtattccct ttagacttac tcattttaga
    10261 accatccata agtaccattc cttgagtaag taagttttta aatggctcat tgaagtctac
    10321 cattcccata ctcttaaatg cctttacaaa ccatcttgca taaagtaaat gcattatagc
    10381 atgttctact ccacctatat attggtctac tggatgccat ctattaacta attccttact
    10441 aaatggttca ttttcattat tgctatccac atatcttaag aaataccaag aagaatcaac
    10501 gaatgtatcc atagtatcaa cttcccttct agctggtttt ccacagtgag gacaagttgt
    10561 tgacataaat tgctttgaag tcgtaagtgg agattcccct tttccagtaa attcaacatc
    10621 tgttggtaaa agaactggta aatcttcctt tttaactgga actattccac attcatcaca
    10681 ataaaccaca ggtataggac aaccccagta tctttgtctt gaaactaacc agtctctaag
    10741 tctataatta attgttctct ttcctctacc ttctttttct aacttatcga ttattccttc
    10801 aaatccttct gaagcatcta atccattaaa ttcttctgaa tttatcattt tgttatcttc
    10861 tgttattaca tcaattatgt ctaaattata tttttcagca aaatctctat ctctttcatc
    10921 atgtgctgga actgccatta tagcacctgt tccatactca actaatacat agttagctat
    10981 ccatagagga actttcttgc catttaaagg atttataaca tatctaccta taaacattcc
    11041 ttctttttct acatcagctg ctgttctttc tatttctgtc atagtatgca ttttttgaac
    11101 aaaagcttca acatctgctt catattcagt ccctgcaacc aattctttaa ctaattcatg
    11161 ctctggagca agtaccatat aagtaactcc ataagttgtg tctggtctag ttgtaaatac
    11221 tgtcatagat ttatcagttc catctatatc aaaaactaaa tctgctcctg tacttttacc
    11281 tatccaattt ttttgcattg ttttaacttt ttctggccat ccatctaata tatctaagtc
    11341 attaagtaat tcttctgcaa attcagtagt cttaaaatac cattgttcta aatctttctt
    11401 taatactgtt gctttacatc tctcacatgc tccttgaaca acttgctcat ttgcaagaac
    11461 agtctcacaa gaaggacacc agttaacata tgattttttc ttatatgcaa gcccatgctc
    11521 taaaaacttc aaaaatattt cttgtgtaaa tctatagtat tctggcgttg aagttgcaac
    11581 ttctttatcc caatcataac taatacctaa taaatttaat tgctctttca tttcttctat
    11641 attttccatt gtccatttat gtggatgtat tccatgtttt atagctgcat tttcagctgg
    11701 taatccaaaa gaatcccatc ccattggatg aagtacatta tatccttcca tttttttgaa
    11761 tcttgcgaca acatcaccta tagaataatt tctaacatgt cccatgtgta tttttccaga
    11821 tggataaggg aacatttcta atgtgtaata attaggtttt tcagtttgtg cagtatccat
    11881 tttatactga ttattatctt tccagatttt ttgccatttc gattctactt ctttaaaatt
    11941 atatacgctc atattttgcc tcctattttt aattgtgtat aactacctta tttagtatta
    12001 tttacttata atttccaaat aattctttga aaataaaaaa tcctcgtcac taaacttacg
    12061 tttagggacg aggataaatc ttcgcggtac caccctaatt agcctactac atatatagac
    12121 tctcttagat agcttttaat ccagccatgg agaatagatt ttaatctagt aagttcaaag
    12181 ttatataata ttagttctca ccttccacta actctctgaa aaaatataat cttttactgc
    12241 tctagtctat tcaatttaat ataatttttg aaattttcta attataataa catatattat
    12301 catgtttatg ctaatgtgtc aatatctata taaggtgcat ctttccataa tttttctaaa
    12361 ttataaaacc ctctattttc ctcattaaat acatgaacca ttatgtctcc ataatcaagg
    12421 agtacccaag tttgagttcc ttgtccttct ttacctcttg gctctaatcc tagttttgct
    12481 aattcatctt ccacattgtc agcaatagct tttacctgtc tctgagaaga tgctgtagta
    12541 ataataaaat aatcacacaa actagaaact tttcctatat ttattataac tgtatcttga
    12601 cccagtttat cttctatggc atcatatgct atttttgtca tttgttccac tgtcatataa
    12661 aaattcctca ctttcctaat taaaatacac ttagttattg taacatatta atacgatttt
    12721 gtaacatagt caaatttaaa tttattaata tgtttgcctt tatatagctt ttttataata
    12781 acttttcttt aattagatag tttcttgcca taactgttct tggatgtatc tcttcttttt
    12841 tattgatgac aaacattaaa gtattattaa aagaagttaa aagtgcttca tcaagcttcc
    12901 ctccatatgc taattctctt aatgtatcta caactgggaa ttttcttcct tcttcaatta
    12961 aatcagctat gtaaattatt ttttctaaaa gagacatgtc ctctctccct gttgtatgat
    13021 atttgattgc atttataata tcttcatctt ccacttcaaa ttcatactcg catatgtacg
    13081 ccccaattac actatgagac aaggaacgat ttccatcctc tattgggtct aaatatattt
    13141 catacttatt tacataatcc tctatttcct tgtcacttaa atactttgca caatcatgaa
    13201 tcattcctgc taagtaagct ttttctttgt cacatccata aatctcacat aatttttcag
    13261 cacattttgc aacatttttt gaatggctaa gcctccccac tggtagcatt tgatttagtc
    13321 tttggttaat attttctaag ttcatttaat catctccata ttgatatagt ttattttcat
    13381 ataaataatt ttctacattt tcgggtacta agtaacgtat tgacttgcct tcatttagct
    13441 gttcccttat atatgtggac gatatatcca gtgatggaac ataaactgtt ataatgtcag
    13501 cattatactt cttagttagt ttttctatag tctcttgtga ccttaacaaa ctaattcctg
    13561 gtctagttgc ggctataaaa gtagctaact caaagttttt ctttacatct ttccactctt
    13621 caacactaca aattgcatct gcacctgtta taaaataaat gtctgcattt ttgtattttt
    13681 tctttaagta ttttaatgta tctattgtat aagtcctttt ctttctttcg atttctactt
    13741 ttgaaactaa aaaatcttcg ttgctttcag ttgctagaag tgtcatatta tatctatcat
    13801 acttatctgt agtgatacat aatttatgtg gtggatttcc tgatggtata aagattattt
    13861 tgtcaatgtc atacttgtct ctaataaatt ctgctgttgc taaatgagca tagtgtatag
    13921 ggtcaaaagt acctcccaat atacctattt tgattttgcc tttatggcga ttaaatttct
    13981 ctagtttttc agtatttttc aactctgcca tcttaactag attttcactt ctcatatttt
    14041 gtctcctttt aatgttataa tactattgta ccattatttt ttatttctta aagcttataa
    14101 tttgtttttt tgggtatata attatattta tatgctaaaa aacactttaa aaaataatta
    14161 tttaacatga ttctgcttta ctttttaata aaaataactt tttactattt tgtcttaatt
    14221 ttgataaaat ataacataca aatataagtt ttttgttaaa ttttattagt tgcttttgtt
    14281 aatataatta catataaaaa aggaggactt atgggtataa ttacaaaaat agaacaacaa
    14341 aaaagaaatg atgatagagt taatatttat gtagatgata aattttttat tgctatattt
    14401 aaagaactgg tttatacatt taatttaaaa aaagggaatg aaataaatga agaagaatta
    14461 aaacctattt tagatgatga aatgtatata aaagctaaaa ataaagcatt aaatatttta
    14521 tctcatgctg accaatctga aaaaaaatta aaagaaaaac tatcatcaga atttgatgaa
    14581 aatataattg agagggtcat agattttctt aaaagttata atctcgtaaa tgacagtgta
    14641 ttagctcaaa aaattgttaa cacaaatgtt aacctaaata aatgtggcaa aaacagaatt
    14701 aagcagaatt tatataataa aggtatcaat agaagtacaa tcaatgaagc agtatctgaa
    14761 ttagacaagg acactgaatt tgaaaatgct atgtatctag caaaaaaaag gtatgaaaga
    14821 gtaaagaaag aagataaaaa gaaaatatat caaaaaattt ctcaacatct ttcctataaa
    14881 ggctttgatt atgatattat taaaagagtt ttaaacaaac ttttaaattt tgatgaatac
    14941 gatatataag tataaatact agggaggtaa attatgaaat taatcattgg tggcttaa
//
LOCUS       DAELLV010000081        14768 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.081, whole genome shotgun sequence.
ACCESSION   DAELLV010000081 DAELLV010000000
VERSION     DAELLV010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14768
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.081"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            114..671
                     /locus_tag="KPD58_001932"
     CDS             114..671
                     /locus_tag="KPD58_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0265698.1"
                     /translation="MRVSREIVIQATSDIVNKDGLNKVSLKVIAEKLGIRTPSLYNHI
                     ESLDDLLLEVAHKGMREMNSQMIKAAIGNFGDTAIKCISIAYFNFIISNPGVYETIQW
                     ATWHGNNETLEIFDEYKSLLEKLILSCNLNTKNTGEVLNLIMSVLHGYSTLQLGKSLL
                     SKEEAIKGLVDSIDTVLLGIHKKYD"
     gene            complement(1021..1383)
                     /locus_tag="KPD58_001933"
     CDS             complement(1021..1383)
                     /locus_tag="KPD58_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0265699.1"
                     /translation="MEIDYKAIGQRIKIARIKKGITQESVANIINMTPSHMSNVETGK
                     TKVSLPTLIAIANALSVSVDTLLCDNVLSSKVIFEKEAKDIFSDCDEYEIRSLVELLK
                     SAKTVIRKDREIRKQFEK"
     gene            complement(1817..2626)
                     /locus_tag="KPD58_001934"
     CDS             complement(1817..2626)
                     /locus_tag="KPD58_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0265700.1"
                     /translation="MIKKLYKTSEFAELCGVKKHTLYHYDEIELLKPSITHENGYRYY
                     TIDQFEKFNIISVLKKAGTPLEEIKQYLNDLDTNTFLNVLHRKLMDLQEEVKKIEKMC
                     SMLRNVISTMEIHLNVNTEKIEIVNCEEEYLIATKIPEAVLTDEKTMLQGIGNHLQYC
                     LLHDYNFHVGEIVLEKNIKQGIFRESYYYSLIKNKVDDERLFIKPKGNYAVMYHQGSY
                     DSLYKTYQNMKNQLEEQGYHIVGNIYEEDLIDYLSEHNAENYIQKISMQVI"
     gene            2718..3341
                     /locus_tag="KPD58_001935"
     CDS             2718..3341
                     /locus_tag="KPD58_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HBG0265701.1"
                     /translation="MKIITISREFGSGGRELGKQLADILNFDYYDKEIITAIARKKKI
                     DENYVSYILENHGWRNVPLTMCTSFTNILSMQSMKTDLLIEQSNVINQIASTGKDCVI
                     VGRNADILLKKYQPFNVFVCANMNFKIQRCIEKKLNNEHISKKELERKIREIDKNRAR
                     SREFFTSSLWGNQTSYHIIVNTSEWNIKHLSYAIANFSEYWFRRYEQ"
     gene            3338..4663
                     /locus_tag="KPD58_001936"
     CDS             3338..4663
                     /locus_tag="KPD58_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009295908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG0265702.1"
                     /translation="MIQLSDNFNYKRLLKFTFPTIIMLVISSIYGVVDGYFVSNFVGK
                     TAFTAVNFIMPFLLILGCVGFLFGTGGGALIAKTMGEGKKEEANEQFSLLIVTSAGCG
                     IILAVLGIVTMPWIASAMGADGQLLTDSILYGCVVIIAIPAYILQCEFQCLFATAEKP
                     KLGLYVAIMAGITNIVLDALFITVFKWGLVGAALATAIGQYVGGIIPLLYFSRQNNSL
                     LKFKKPKFDCDVLKKTVANGSSELMTNVSTSVVSMLYNAQLLKYAGEDGVASYGVLLY
                     VALLFQAVFIGYSVGTAPIISYHYGAKNYDELKNLRKRSLIVIICFAVFMFIIGEFLS
                     KPIAFIFVGYDNNLMEMTLRAFMIYSFSFLFSGFGVFGSSFFTALNDGLTSALIAFMR
                     ILVFQVVSVLIFPIFWGVNGIWFSIVGAEILATIMTAFFIIRKQKKYHY"
     gene            4684..5223
                     /locus_tag="KPD58_001937"
     CDS             4684..5223
                     /locus_tag="KPD58_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0265703.1"
                     /translation="MSLTIQKVTSYFKDLEKFEELNNEAFPDEERMSIQEMLNLVQSK
                     KIEVSALYDKQQFIGFYVLMIENHIGYILFLAISPEQRSKGYGGKALSLMKKQYPNCQ
                     IVLDMETIEDSAPNLQQRILRKQFYLQNGFYETEYLMEYKGLEMEVLCNQKTFQKNEF
                     ETLLKNLKIREIPFHLWKK"
     gene            5242..6633
                     /locus_tag="KPD58_001938"
     CDS             5242..6633
                     /locus_tag="KPD58_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HBG0265704.1"
                     /translation="MKRRLAGEWEPIKGVMLAWPPFLPHKYIVDLTQNYSVYMCVPNK
                     EEIEIAKYYLSKWGGNLKNLIFCIAPQGFDTPWVRDWGMHPIFDENGKLFLAGAEYKM
                     STPFVSINKPEIMFDCEHNLLTTNSYEQKEDQSQELIAKQIGLPFMKLPYALTGGNLM
                     SDGFNKLMSMHILQTENRAKGISDEEFFSMVARDTGMNEYYIFSNFANYGLQHIDCYL
                     KIIDEETLLVSRTPKEHYLYERYEKILEEVKKVESRYGRPFKIFRIDIVPFQKDSDEL
                     TAYVNSVILNSRVYVPMYGIEQDKIAIKQWQNALPGYEIKGYEFDFEKEPDIIKNRTG
                     YVRTGWGNEDVIHCRTRAIWDENMLYISVKRLEEIVSENDALEVTIQIVDYSKAGLDY
                     ENCRLFYRYYGFQTWESIRLEETTEAEIFFANMIGKSGDMIEYFVQAKSCSGMCETMP
                     PVAPKGTYKVTIK"
     gene            6648..7739
                     /gene="ytvI"
                     /locus_tag="KPD58_001939"
     CDS             6648..7739
                     /gene="ytvI"
                     /locus_tag="KPD58_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HBG0265705.1"
                     /translation="MNEQDHTSYQNKQRFLINFCYLVVILGLLMLLGKYAFSVLLPFV
                     FAFLIASLLNKPVLYISEKLLIKRGIVATISVFLFFLIAGIIVSVIGTYLIYGIKEIF
                     HFLPTMFEELVIPLTETVIKEVELFSGSFNFSFIELIEANMPVLMNEMSVFIVSLSNN
                     IVSGITDIVSSVPLLFMKTIITIVASIFIAIDYPTIKRFIVLQIPKNKSYLLAEAKAF
                     TIDTIIKCGFSYLLIFAITFSELYVGFVIINISYAGIIALFIALLDILPVLGTGSILI
                     PWCIISLFLGDYSMALGIALLYIVITIIRNIIEPKLVGKHMELHPVLALASMLTGLHF
                     FGFIGLFGIPLLIAFLKKLNDKEIIHILH"
     misc_binding    8042..8255
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8509..9543
                     /locus_tag="KPD58_001940"
     CDS             8509..9543
                     /locus_tag="KPD58_001940"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HBG0265706.1"
                     /translation="MNKIKVGIIGATGYVGAELIRLLMNHDKVEVTAIGSNSYVGKDI
                     VDIYPSIGYKNNMICIENEKVIDMCDVVFTALPHGVSEKFVIKAIKSKKKVIDLGADF
                     RIKDEEVYSKWYGVSFIDKILHKKAVYGLSEIYKEDIKDADIIANPGCYPTSISLPLM
                     PLLSSKLIKNNNIIIDSKSGLTGAGRELSISSHFTEVNENITAYKIGKHRHTPEIEQN
                     LSESCKEKVSVVFTPNLIPVNRGILSTIYCTKEDNISINDIHRKLTDYYEFKEFIEIL
                     PLDKVASLKNVRFSNKCVISLHENGDTLIICSAIDNMIKGSAGQAIQNMNIIFGIEEN
                     TGLKNIAPSF"
     gene            9584..10801
                     /gene="argJ"
                     /locus_tag="KPD58_001941"
     CDS             9584..10801
                     /gene="argJ"
                     /locus_tag="KPD58_001941"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HBG0265707.1"
                     /translation="MEIIKGGVTVSEGFFASGIHCGLRKNKEKRDLALVYSDVLCDAA
                     AVVTQNKVKGNPVYVTQEHIKNGKAQAIIVNSANANTFNGKEGLINAYKMAKFTSDKL
                     NIKESDVLVASTGVIGKPLNIDLIEENMDELVNNLSKQGHIGAREAIMTTDIIKKEIA
                     VAIMYGDKKITIGGMAKGSGMIHPNMATTLGFITTDANIDGVLLKEALKIAVDKSFNR
                     VSVDGDTSTNDMVMILANGKAKNDRINKKDEHYQVFLSALTYVCIELAKLVAKDGEGC
                     TKLIECYINGALSEEHAVKLAKTVISSSLVKTAVFGADANWGRILCALGYAGEEIDME
                     KVDIIFESMKGYIEVCKNGNGLDFNEEKAKKILEDDEISILVDLNMGNARGNAWGCDL
                     SYDYVRINGSYRN"
     gene            10816..11676
                     /gene="argB"
                     /locus_tag="KPD58_001942"
     CDS             10816..11676
                     /gene="argB"
                     /locus_tag="KPD58_001942"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HBG0265708.1"
                     /translation="MINIEKANTLIEALPYIEKHQGKTIVVKYGGSAMKKDGLKESVM
                     EDLVLMSYVGINIVLVHGGGAEINKMLAKVDIESKFVNGLRYTDEETMEIVKMVLAGK
                     VNKDLVNKIHTKGGKAVGLCGIDNNMILCDPYKNYELGFVGEIKKVNVELIESCLKSG
                     YISVIATIGVGDDGETYNINGDTAASAIAKELNADKLILLTDVPGLLREPDEEKSLIT
                     EVILEDVDKLFEEGIITGGMIPKIEGCVDALNNGVNRVHILDGRVPHSIITELFTDSG
                     IGTLIRKENE"
     gene            11706..12893
                     /locus_tag="KPD58_001943"
     CDS             11706..12893
                     /locus_tag="KPD58_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HBG0265709.1"
                     /translation="MNNDNTISKWNEYFIDTYNQPNFVIDYGEGSCFFDTNGNKYIDF
                     TSGYGVSSLGYSNSNLKNALKEQVDKLLHTSNLYFNEPVLCSGEKIINSSGMAKVYFC
                     NSGTEANETAFKIARKYSSDKYGNGRGTIISLKDSFHGRTMMSLMATGMDKYHKYFYP
                     LPEGFKYIERNNIEDLKNNLDSTVCAIILEAIQGEGGVNVLEKDYVLEIVKICQEKDI
                     VVIFDEVQCGIGRTGKLFGYEYFDVKPDIVTVAKGLGAGIPVGGVLVNKKLSKVLGKG
                     DQGTTFGGNLLAMVAASVVLDEISKDGFYNEVLEKGNYIRKSIESFNNKVVLKTKGIG
                     LMIGIETNIESSIIEEKARKKGLLILTAGKNVLRFLPPLTISYKEIDEALEILKDILL
                     EIN"
     gene            12997..14019
                     /gene="argF"
                     /locus_tag="KPD58_001944"
     CDS             12997..14019
                     /gene="argF"
                     /locus_tag="KPD58_001944"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBG0265710.1"
                     /translation="MNNISLKGKSFLTLKDFTKEEIRYLLTLSSYLKTKKKVGIKGDL
                     LQGKNIALLFEKTSTRTRCSFEVAAHDEGAHVTYLGPNDCHMGKKESVSDTAKVLGRF
                     YDGIEFRGFKQETVESLAKYSGVPVWNGLTDEYHPTQILADFLTVIENVDKDLNKVKF
                     VYVGDARNNMGNSLMIGCAKMGIHFVALAPKELWPNEELVKEMKEIAKKSKGDIMLTE
                     NIDDVKGADVIYTDVWVSMGEEEQYEARINQLKDYQVNMDMIKKTENENVIFLHCLPA
                     FHDLETKVAQEIYEKFGLSELEVTNEVFNSKYSKVFEEAENRMHTIKAVMVSTMTDVN
                     MNNNLV"
     gene            14146..14763
                     /locus_tag="KPD58_001945"
     CDS             14146..14763
                     /locus_tag="KPD58_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HBG0265711.1"
                     /translation="MKKIILALTTFLLAISLTACSSAKPEDTIESFFNSAKKFDFEGM
                     NKVMENNDEKYKDILKELDTKDPNAQYVLDYLKQNASKITYTIKDSEVKGDKATIKVE
                     CKFIDSTPLLQEIVAEAFTKMIGMSFSGQDLTDEKTTEMLVSIMKEKQKSVKETYVTK
                     NVEFECSKKDNKWIISSVNDAVADVLLSNLVTAGQEFSNSMNTLE"
BASE COUNT         5516 a         1635 c         2674 g         4943 t
ORIGIN      
        1 ctgtcaagat tttataatag tataaagaat tttataaatt acaataattg acgaaactaa
       61 ttagattagt taaactaata aagtaattaa ttttaaatga aagagggaaa atcatgcgtg
      121 ttagtagaga aatagttata caagctacat cagatatagt taataaagat gggcttaaca
      181 aagtttcttt aaaagttatt gcagaaaagc taggaatacg tacaccatct ttgtacaatc
      241 atatagaaag tctagatgat ttattacttg aagtagctca taaaggaatg agagagatga
      301 atagtcaaat gataaaggcg gctattggaa attttggaga tacagctata aagtgtataa
      361 gtatagcata ctttaatttt ataatttcta atcctggtgt ttatgaaaca attcagtggg
      421 ctacttggca tggaaataat gaaacattag aaatctttga tgaatataaa tccttattag
      481 aaaaacttat tctttcttgt aatctaaata caaagaatac aggtgaagta ttaaacttaa
      541 taatgagtgt acttcatggt tattcgactt tgcaacttgg gaaatcactt ttgagtaaag
      601 aagaagctat aaaaggtcta gtcgattcta ttgatactgt gttattaggt attcataaaa
      661 aatatgatta aggaggtaaa taaattattt taacttattt attgaacact tcattacgtg
      721 ggatgtaaaa taaaatttta tcatttttat gaaaggaaaa tagtatattt caagaatatt
      781 tactttctga attggtaaac cctattttta ctgttgtcgt tgataatgaa aaggtagctg
      841 taaaattcct tgataatcag acaaaagcga cacaggtatc atagtataaa ttgatatttc
      901 ataaggacag taattggaaa attattggat aggttatttg attatataaa aatgattata
      961 taaaaaaaga aaagacctta gctaacaaca catatcaatt tataccttga agctgtggtt
     1021 ttatttttca aattgtttgc ggatttctct gtctttacgg attacagttt ttgctgattt
     1081 tagcagttca actaaagaac ggatttcata ttcatcacaa tctgagaaaa tatctttggc
     1141 ttccttttca aaaattactt tggaagaaag tacattatca cataagagag tatcgactga
     1201 tacagaaagt gcattggcaa tcgcaatcaa ggttggcagg ctgactttcg tttttcctgt
     1261 ttccacgttg ctcatatggg atggtgtcat gttgataatg tttgcaacgg attcttgtgt
     1321 tatacctttt ttaattcttg caattttaat tcgctgtccg attgctttat aatcaatttc
     1381 catagttttt gttctccttt cattctttta aatattgtaa ctatttacag agcataatat
     1441 aatactctat ataaatgaat aatttatata atatatattt aagaaaaaaa tttgcactac
     1501 ttttaagaca actacaaaag aaatggaggc ataaagcctc catgttaaga ttttataaaa
     1561 agatagtaaa aaactgatta aatggttaac gaaaataaaa agctatttct gtatctgaaa
     1621 ttcatgagga aatgcagttt gcattgagga aacaaataat agcaatgaac ctgaatttct
     1681 acaaaaattt ggaaataaca taccaacttt agaagataaa aaaggacatt ttaagcaaat
     1741 tattatagac aatacgagat attaaaaaag gcgtgtattt ctcatgtgat ttgagatata
     1801 cacgcttttt atattttcaa ataacctgca tagaaatttt ttgaatgtag ttttcggcgt
     1861 tatgttctga taaataatca attaaatctt cttcataaat atttccaaca atatgatacc
     1921 cttgttcttc taactgattt ttcatatttt gataagtttt atataaactg tcataactcc
     1981 cctggtgata cataactgca taatttccct ttggtttaat aaaaaggcgt tcatcatcta
     2041 ctttattttt aataaggcta taataatagc tctctctaaa aattccctgt tttatatttt
     2101 tttcaagaac aatttctcct acatgaaaat tataatcatg taataaacaa tattgtaagt
     2161 gatttccaat accttgtaac attgtttttt catctgttag tacagcttca ggaattttag
     2221 ttgcaatcag atattcttcc tcacaattta caatttcgat tttttctgtg tttacattga
     2281 gatgtatttc cattgttgaa atgacatttc tcaacataga acacattttt tcaatcttct
     2341 tcacttcttc ttgaagatcc attagctttc tgtgaagtac attaagaaag gtattggtat
     2401 ctaaatcatt gagatattgt tttatttctt ctaaaggtgt acctgctttt tttagcacag
     2461 aaataatatt aaatttttca aattggtcaa ttgtataata ccgatagcca ttttcatggg
     2521 taatactcgg ttttaataat tcaatttcat cataatggta aagtgtatgt ttttttaccc
     2581 cacacaattc tgcaaattca cttgtcttat ataatttttt aatcattttt aaatctccta
     2641 ttgactttag ggttaccaga tactttagga taacatagaa agcttttata ttcaatgaaa
     2701 tgtgaggaaa agatattatg aaaattatta caattagccg agagtttgga agtggtggac
     2761 gagaacttgg aaaacaactt gctgatattt taaattttga ctactacgat aaagaaatta
     2821 tcactgctat agctagaaag aaaaaaattg acgaaaacta tgtttcctac atattagaaa
     2881 atcatggttg gagaaacgtt cctttaacaa tgtgtacttc ttttactaat attttgtcta
     2941 tgcaatccat gaagacagat ttgttaatag aacaatcaaa tgttatcaat cagattgcca
     3001 gtacaggtaa ggattgtgtt attgttggta gaaatgcaga tatattatta aagaagtacc
     3061 agccatttaa cgtttttgtt tgtgcaaata tgaactttaa aatacaacgc tgtattgaaa
     3121 aaaagttaaa caatgaacat atttcaaaaa aagaattaga aagaaagata agagagattg
     3181 ataaaaatcg agcaagatca agagaatttt ttacgagctc cttatgggga aatcaaactt
     3241 cctatcatat tatagttaat acatctgaat ggaatataaa acatctttct tatgcaattg
     3301 ctaacttttc agagtattgg tttaggaggt atgaacaatg atacagttgt cagataattt
     3361 taattataaa cgcttattaa aatttacatt tcctacaatt attatgctgg taataagttc
     3421 tatttatggg gttgttgatg gatattttgt ttcaaatttt gtagggaaaa ctgcttttac
     3481 tgctgtcaat tttattatgc catttttgct aatcttaggg tgtgttggct ttctttttgg
     3541 aacaggtggt ggagctttaa ttgctaaaac aatgggtgaa ggaaaaaaag aagaagcaaa
     3601 cgaacagttt tcgttactca ttgttacttc tgctggttgt ggtattattc tagctgtttt
     3661 aggtatagta actatgccat ggattgcttc tgctatggga gcagatggtc aattattgac
     3721 agatagtatt ctttatggat gtgtggtaat tatcgctatt cctgcctata ttttgcagtg
     3781 cgaatttcag tgtttatttg caacagcaga aaagccaaaa cttggattat atgttgcaat
     3841 tatggctgga attacaaata ttgttttaga cgcattattt atcactgttt ttaaatgggg
     3901 attggtaggt gcagcactgg caactgcaat cggtcaatat gttggtggta tcattccatt
     3961 actttatttt agccgacaaa acaacagttt gcttaaattt aagaaaccta aatttgattg
     4021 tgatgttttg aaaaagactg ttgcaaatgg ttcttcagaa ctaatgacta atgtttccac
     4081 atctgttgtg agtatgctgt ataatgctca attactaaaa tatgctggcg aagatggcgt
     4141 tgcatcctat ggtgttcttc tttatgtagc gttactattt caagctgttt ttattgggta
     4201 ttctgttggt actgccccta taatcagtta tcattatgga gcaaaaaatt atgacgaatt
     4261 aaaaaattta agaaaaagaa gtttgattgt aattatttgt tttgctgtat ttatgtttat
     4321 aataggcgaa tttttatcaa aaccgattgc ttttattttt gtgggttacg ataataattt
     4381 aatggaaatg acattaagag catttatgat ttactctttt tcctttttat tttctggttt
     4441 tggtgttttt ggttctagtt tctttactgc attaaatgac ggtttaactt ctgcgcttat
     4501 tgcttttatg cgtattttag tatttcaagt tgtatcagta cttatattcc ctatattctg
     4561 gggagtaaat gggatatggt tttctattgt tggtgctgaa atattggcaa ctattatgac
     4621 ggctttcttt ataataagaa aacagaaaaa atatcattat taaatcaatt tggaggtatc
     4681 tatatgagct taacaataca aaaagtaact tcatatttta aagatttaga aaaatttgaa
     4741 gaacttaata acgaggcatt tccagatgaa gaacgtatgt ctatacaaga aatgcttaat
     4801 ttagttcaaa gtaaaaaaat agaagtatct gctttatatg ataagcaaca atttattggt
     4861 ttttatgttt tgatgattga aaatcatata ggatatatat tatttcttgc aatttcccca
     4921 gaacaacgct ctaaaggata tggtggaaaa gcactgtcac ttatgaaaaa acaatatccc
     4981 aattgtcaaa ttgttcttga tatggaaact atagaggaca gtgctcctaa tttacagcaa
     5041 agaattttaa ggaagcaatt ttatcttcag aatgggtttt atgaaacaga atatctcatg
     5101 gagtataagg gactagaaat ggaagtttta tgtaatcaga aaacctttca aaaaaatgaa
     5161 tttgaaacat tgttgaaaaa tcttaaaatt agagaaattc catttcattt gtggaaaaag
     5221 taagaaagtg aggtattaga aatgaaaaga cgtttagctg gggaatggga accgattaaa
     5281 ggtgtcatgc ttgcatggcc accttttctt ccccataagt atattgtaga tttaacgcaa
     5341 aattattctg tatacatgtg tgtaccaaat aaagaggaaa ttgaaattgc aaaatattat
     5401 ttaagtaagt ggggtggaaa tttaaaaaat ttgattttct gcattgctcc acagggattt
     5461 gatacaccat gggttcgtga ttggggtatg caccctattt ttgatgaaaa cggaaaactc
     5521 tttttggctg gtgcagaata taagatgtca acaccatttg tatccataaa taaaccagaa
     5581 atcatgtttg attgcgaaca taacctatta actactaact cttatgaaca aaaagaagat
     5641 caatcacaag agttgattgc gaaacagatt ggtttgccat ttatgaaatt accttatgca
     5701 ctaacaggcg gaaatcttat gtcagatggt ttcaataaat tgatgtctat gcacatttta
     5761 cagacagaaa atcgtgcgaa aggaatttct gacgaagaat ttttttctat ggtggcaaga
     5821 gatactggaa tgaatgagta ttatatattt tctaattttg ctaattatgg tttacaacat
     5881 attgactgtt atttaaaaat aattgatgaa gaaacattac ttgtgagcag aacgccaaaa
     5941 gaacactatt tgtatgaaag atatgaaaaa attttagaag aagtaaaaaa ggtagaaagc
     6001 agatatggac gtccattcaa aatctttcgt atagatattg ttccgtttca aaaggacagt
     6061 gatgaactta ctgcttatgt taattctgtg attttaaata gtcgtgtcta tgttccaatg
     6121 tatggaattg aacaggataa aattgcaata aaacaatggc agaacgcttt gcctggatat
     6181 gaaattaaag gatatgagtt tgattttgaa aaagagccag atattataaa aaatcgtaca
     6241 ggatatgtga gaactggctg gggaaatgaa gatgttattc attgcaggac aagagcaatt
     6301 tgggatgaga atatgctcta tatttccgtg aaaagattag aagaaatcgt ttcagagaat
     6361 gatgctttag aagttactat tcaaatagtt gattatagta aagctggctt agattatgaa
     6421 aattgtagac tgttttatcg atactatggt tttcaaacat gggaaagtat taggttagaa
     6481 gaaactacag aagcagaaat attttttgcc aatatgatag gaaagtcagg tgatatgatt
     6541 gaatattttg tgcaagcaaa aagttgttct ggaatgtgtg aaacaatgcc ccctgttgct
     6601 ccaaaaggca catataaagt tactataaag taggaggtgc attttatttg aatgaacaag
     6661 atcatacatc ataccaaaat aagcagagat ttttaataaa tttctgttat ttggtagtga
     6721 tattaggctt actaatgtta ttagggaaat atgcgttttc tgtattactt ccttttgttt
     6781 ttgcattttt aatagcaagt ttattgaata aaccagtctt atacataagt gaaaaattac
     6841 taataaagcg aggtattgta gcaaccatat ctgttttttt attcttttta attgcaggaa
     6901 taatagtaag tgtaataggt acatatctaa tatatggaat aaaggaaatt tttcatttcc
     6961 taccaaccat gtttgaggaa ttggtaatac ctttgactga aacagttata aaagaagtcg
     7021 aactattttc tggttcattc aatttttcgt ttattgaatt aatagaagca aatatgcctg
     7081 tattgatgaa tgaaatgagt gttttcatag tttctctttc aaataatatc gtatctggta
     7141 ttactgatat tgtttcgtct gttccattac tatttatgaa aacaattatt acaatcgttg
     7201 cttctatttt tatagcaatt gattatccaa cgataaagag atttatagtg ctacagatac
     7261 ctaaaaataa aagttatctt ttagcagaag caaaagcgtt tactattgat accattatta
     7321 aatgtggttt ttcttatctt ttaatttttg ctatcacttt ttctgaatta tatgtcggtt
     7381 ttgttattat taatatttct tatgctggaa taattgcttt attcattgct cttttagata
     7441 tactacctgt tttaggaact ggaagtattc taataccttg gtgtattatc agtttatttt
     7501 taggggatta ttcaatggca ttaggaattg cactactata tattgtgatt actattatca
     7561 ggaatattat tgaaccaaaa ttagttggta aacatatgga gttacatcct gtattggcct
     7621 tagccagtat gctaacagga ttacatttct ttggatttat tgggttgttc ggtattccat
     7681 tgttaattgc gtttttgaaa aagttaaatg acaaagaaat catacatata ttacattagg
     7741 aaagaatagt tgtatgtatg tctcttttat ggagaaaatt gtgttttttc taaaaaacgc
     7801 tggatgaaaa ataattttga caaaggtata gcttttgaat gacttgattc aagagggaga
     7861 acttttttaa aatatattac agcaggaaat tcatggtgtc ctattactgt agatatttat
     7921 aactcaattt agatacaaaa tgctggaaat taattatata gaaaatattt ccaaaaactt
     7981 aaaaattata ttgacaaagt taaaaaaata aactatcata taaaacaata agaaaataaa
     8041 ttgaaatcct gtgaaaaaga gagtagcata aagctagttg atagagagct gggaatggtg
     8101 gaagcctagt aacgaatttt attgtgaata gagctttgga ggagattacc agaagtaata
     8161 gtatggtaat tggtgaatct cgccttaaga gattagagtg ggtaagatta tcttatcaat
     8221 tagagtggta acgcgggtgt atattcgtct cttggtcttt gaccaagagg cttttttaat
     8281 tttaaatgta cattttgtag atacttttta atttgagttt taatgattat tgtaatttta
     8341 ccaataagag tggtaccgcg gaaaatcgcc tcttagtatt tataatgcta aggggttttt
     8401 tgttttaatt agagtatttt aatctacgta ttatcaaatg attatcatat agtaaagtgg
     8461 aaagatagga ttttaagact atttgaatat aaaaatggag ggaacaaaat gaataaaata
     8521 aaagttggta ttattggtgc aactggatat gtcggggcag agttgattag attattaatg
     8581 aatcatgata aagtcgaagt gacagcgata ggttcaaatt cgtatgttgg aaaagatata
     8641 gttgatatat atccaagtat aggatataaa aataacatga tatgtataga aaatgaaaaa
     8701 gttatagata tgtgtgatgt tgtgtttaca gcccttccac atggagttag tgaaaagttt
     8761 gtaataaagg caattaaatc gaaaaagaaa gttatagatt taggtgctga ctttagaata
     8821 aaagatgaag aagtatatag taaatggtat ggagtatctt ttatagataa aatcttacat
     8881 aaaaaagctg tatatggatt aagtgaaata tacaaagaag atataaaaga tgcagatata
     8941 attgcaaatc ctggatgtta tccaactagt atttctttac cattgatgcc attactaagt
     9001 tctaaattga taaaaaataa taatataata attgattcta aatcaggtct tacaggtgct
     9061 ggtagagaat tatcgattag tagtcacttt acggaggtta atgaaaatat cactgcatat
     9121 aaaataggga aacacagaca tactccagaa atagaacaaa atttaagtga atcctgcaaa
     9181 gaaaaagtaa gtgtagtttt tacaccaaac ttaatcccag taaatagggg aatcttatca
     9241 actatatatt gtacaaaaga agataatatt tctataaatg atatacatag gaaattaact
     9301 gattattatg aatttaaaga gtttatagaa attcttccat tagataaggt agcttcttta
     9361 aagaatgtta gattctcaaa taaatgtgta atatcattac atgaaaatgg agatacattg
     9421 ataatctgtt cagctataga taatatgatt aagggatcag caggtcaagc tatacaaaac
     9481 atgaatataa tatttggtat tgaagaaaat acaggtttaa aaaatattgc accatcattt
     9541 tagaaataaa aaatactaaa ctcatataat taggaggata aatatggaga ttataaaggg
     9601 tggagttaca gtttcagaag gattttttgc ttcagggatt cactgtggct tgagaaaaaa
     9661 taaagaaaaa agagatttag ctttggttta ttcagatgtt ttatgtgatg cagcagcagt
     9721 agtaactcaa aataaagtaa agggcaatcc tgtttatgtt acacaggaac acattaaaaa
     9781 tggaaaggca caagctatca tagtaaatag tgctaatgct aataccttta atggaaaaga
     9841 aggacttata aatgcttata aaatggctaa attcaccagt gataaattaa atattaaaga
     9901 aagtgatgtt ttggttgcat ctacaggtgt tataggaaag ccattaaata tagatttaat
     9961 agaagaaaat atggatgaat tagtgaataa tctaagtaaa caaggtcata ttggagcaag
    10021 agaagctatt atgacaactg atattataaa aaaagaaata gcagtagcaa taatgtatgg
    10081 agataaaaaa ataactattg gaggtatggc aaaaggttct ggcatgatac atcctaatat
    10141 ggcaacaacg ttaggattta taactacaga tgcaaatatt gatggagttt tgctaaaaga
    10201 agctttaaaa attgctgttg acaaaagttt taatagagtt agtgtagatg gagatacaag
    10261 tactaatgat atggtaatga ttctagcaaa tggaaaagca aaaaatgata gaataaacaa
    10321 aaaagatgaa cattatcaag tatttttgag tgcacttaca tatgtctgta tagaacttgc
    10381 taaacttgtt gcaaaagatg gagaaggatg tacaaaacta attgaatgct atataaatgg
    10441 agcattaagt gaagaacatg cagtaaaatt agcaaaaaca gttatatctt cttcattagt
    10501 aaagacagct gtatttggag ctgacgcaaa ttggggtaga atactgtgtg ctttaggtta
    10561 tgcaggtgaa gaaatagata tggaaaaagt tgatattatt tttgagagta tgaaaggata
    10621 tattgaagta tgtaaaaatg gaaatggatt agattttaat gaggaaaaag caaaaaaaat
    10681 ccttgaagat gacgaaataa gtatattagt agatttaaat atgggcaatg ccagaggaaa
    10741 tgcatgggga tgtgatttaa gctatgatta tgtaagaata aatggaagtt atagaaatta
    10801 ggaggatata ctttaatgat taatatagaa aaagcaaata cacttataga agctttacca
    10861 tatatagaaa aacatcaggg aaagactatt gtagttaagt atggtggaag tgcaatgaaa
    10921 aaagatggtc taaaagaatc tgttatggaa gaccttgtgt taatgagtta tgttggaatc
    10981 aatatagttt tagtacatgg tggaggggca gaaatcaata agatgctagc aaaagtggat
    11041 atagaaagta aatttgtaaa tggtcttaga tatacagatg aagagaccat ggaaattgta
    11101 aaaatggtac ttgctggaaa ggttaataaa gacctagtaa ataaaataca tacaaaaggt
    11161 ggaaaagcag tagggctttg tggaatagat aataatatga ttttatgtga cccatataaa
    11221 aattatgaac ttggatttgt aggagaaatc aaaaaagtaa atgttgagct tatagaatct
    11281 tgtctaaagt caggttatat ttcagtaatt gcaactatag gtgttggaga tgatggagag
    11341 acttataata taaatggtga cacagcagct tctgctatag ctaaagagtt aaatgcagat
    11401 aaattgatac ttcttacaga tgtccctggt ttgttaagag agccagatga agaaaaatca
    11461 ttaataacag aagttatatt agaggatgtt gataaattat ttgaagaagg tataattact
    11521 ggaggtatga tacctaaaat agagggatgc gtagatgcct taaataacgg agtaaatagg
    11581 gtacatattt tggatggaag agttccacat tctataataa cagagctatt tactgatagt
    11641 ggaataggta cattaataag aaaagaaaat gaataaaatt ataagtttaa gaggggagaa
    11701 taagaatgaa taacgacaat acaatttcaa aatggaatga atattttata gatacatata
    11761 accaacccaa ttttgtcata gattatggtg aaggaagttg tttttttgat actaatggca
    11821 ataaatacat agattttaca agtggatatg gagtatcttc tcttgggtat agtaatagta
    11881 atttaaagaa tgctctaaaa gagcaagtag ataaattatt acatacttca aatttatatt
    11941 ttaatgagcc agtgttgtgt tcaggagaga aaataattaa ttcaagtgga atggctaaag
    12001 tatatttttg taattctgga acagaagcaa atgaaacagc attcaaaata gcaagaaagt
    12061 atagtagtga caaatatggt aatggtagag gaactatcat aagtttaaaa gactcttttc
    12121 atggtagaac aatgatgtct ttaatggcaa ctggtatgga caaataccat aagtattttt
    12181 atcctcttcc cgaaggtttt aaatatatag aaagaaataa tattgaagat ttaaaaaata
    12241 atcttgattc cacagtttgt gcaataattc ttgaagcgat tcaaggcgaa ggtggagtta
    12301 atgttcttga aaaagattat gttttggaaa tagtaaaaat atgtcaagaa aaggatattg
    12361 tagtgatttt tgatgaagtc caatgcggta taggaagaac tggtaaactt tttggctatg
    12421 aatattttga tgtaaaacca gatatagtta ccgtagcaaa agggttggga gcaggaatac
    12481 cagtaggagg agtattggta aataaaaaac tttcaaaagt actaggaaaa ggagaccaag
    12541 gtacaacttt tggaggtaat ttattagcta tggtggcagc ttcagttgta ttagatgaga
    12601 tatctaaaga tggtttttat aatgaagtat tagaaaaagg aaattatata agaaaatcta
    12661 ttgaaagttt taataacaaa gttgtattaa agacaaaagg tattggtcta atgataggaa
    12721 ttgaaactaa tattgaatct agtattatag aagagaaagc tagaaaaaaa ggattgctta
    12781 ttttgactgc tggaaaaaat gtattgagat ttttaccacc attaacaatc agttataagg
    12841 aaatagatga ggctcttgaa atcctaaaag atatattact agaaattaac taaatattca
    12901 aaaaatagtt aattaaaaaa ttagaagtaa ataaaaaagt aaagtgctaa caagtttaat
    12961 tgaatttaaa aagtttataa gaaggggcga attaatatga ataatataag tttaaaaggt
    13021 aagagttttt tgacattaaa ggattttaca aaagaagaga ttagatattt attaacttta
    13081 tcaagttatt taaaaactaa gaaaaaagtt ggtataaaag gcgatttgct acaaggaaaa
    13141 aatatagctt tattatttga gaaaacatca acaagaacta gatgttcatt tgaagtagca
    13201 gctcatgatg aaggagcaca tgtaacttat ctaggtccaa atgattgtca tatgggtaaa
    13261 aaagagtctg tatcagatac agcaaaagtt ctaggaagat tctatgatgg aatagaattt
    13321 agaggcttta aacaagaaac agttgaaagt ctagcaaaat attctggtgt acctgtttgg
    13381 aatggactta cagatgaata tcatccaaca caaatactgg cagatttttt aacagtaatt
    13441 gaaaatgtag ataaagattt gaataaagtt aaatttgtat atgttggtga tgctagaaat
    13501 aatatgggaa actcactgat gataggatgt gctaaaatgg gaatacattt cgtagcacta
    13561 gcacctaagg aactttggcc aaatgaagaa ttggttaagg aaatgaagga aatagccaag
    13621 aaatccaaag gagatataat gttgactgaa aatattgatg atgttaaagg tgcagatgtt
    13681 atatatacag atgtatgggt ttctatggga gaagaggagc aatatgaagc aagaataaat
    13741 caacttaaag attatcaagt aaatatggat atgataaaga aaactgaaaa tgaaaatgtt
    13801 atatttttac actgtcttcc agcatttcat gatttagaaa ctaaggttgc tcaggagatt
    13861 tatgaaaagt tcggattatc agaattagag gttacaaacg aggtatttaa ttcaaaatat
    13921 tcaaaggtat ttgaagaagc tgaaaataga atgcatacaa taaaagctgt tatggtttct
    13981 actatgaccg atgtaaatat gaacaataat ttagtataga tatgttttaa tatagacttt
    14041 actttaatga aaatttataa aatattttaa atatattatt caaaaactct cattatatgt
    14101 taaaataact tatgtatcaa caaattaaga gggaggattt gagatatgaa aaaaatcata
    14161 ttagctttaa caactttttt attagcaata tctttaacag catgttcaag tgcaaaacca
    14221 gaagatacaa ttgagagttt ttttaacagt gcgaaaaagt ttgactttga aggcatgaat
    14281 aaggtaatgg aaaataatga cgaaaaatat aaggatatat taaaggaatt agatacaaaa
    14341 gacccaaatg cccaatatgt tttagattat ctaaaacaga atgcatctaa aatcacatat
    14401 acaataaaag acagcgaggt taaaggtgat aaggctacaa tcaaagtaga atgtaaattt
    14461 atagattcaa caccattact tcaagaaata gtagctgagg catttacaaa gatgattggt
    14521 atgagttttt caggacaaga cttaactgat gagaaaacaa ctgaaatgtt agtatcaatt
    14581 atgaaagaaa aacaaaaaag cgttaaagag acttatgtta caaaaaatgt agagtttgaa
    14641 tgttctaaaa aggataataa atggataata agttcagtaa atgatgcagt agctgatgta
    14701 ttgttatcta atcttgttac agcaggacaa gaattttcta actctatgaa tacacttgaa
    14761 taatattg
//
LOCUS       DAELLV010000082        14581 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.082, whole genome shotgun sequence.
ACCESSION   DAELLV010000082 DAELLV010000000
VERSION     DAELLV010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14581
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.082"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            114..611
                     /locus_tag="KPD58_001946"
     CDS             114..611
                     /locus_tag="KPD58_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBG0265712.1"
                     /translation="MNYEIVEINEKIVIGISKETTNKDGQAVNDIGELWKKFMGKGIY
                     NAIKGKKNDKTIGLYTDYQGDFTSPYSFVACCEVNSNSDKEKNLEELNTNNTVGESII
                     SKVIPAGKYAKFVIVGGQKEVGDFWFEFWQMDFDRTYISDFEEYQCNTFDTKKQEIHI
                     YIGIK"
     gene            1355..1717
                     /locus_tag="KPD58_001947"
     CDS             1355..1717
                     /locus_tag="KPD58_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBG0265713.1"
                     /translation="MENKILIVDDAIFMRMIIKEALSKSGYNNLIEANDGEEACNIFK
                     YEKPDLMLLDITMPKKDGLEVLREIKKLDSSAKVVMCSAIGQENMIVEAIKLGALDFI
                     VKPFKTETLVKVVDNALK"
     gene            1782..2399
                     /locus_tag="KPD58_001948"
     CDS             1782..2399
                     /locus_tag="KPD58_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HBG0265714.1"
                     /translation="MINYLELDDVHIDALREIGNIGSGNAITALASMINSNVEVLIPM
                     VKILEYNEATNLLGGPENKVVCILLDMKGDINGMFMFLLDESITQLMLSSLFNKEEAF
                     LDEIEAIEISAIKEIGNIMASSYVNAIASMLNMTISVSIPDICIDMVGAVLNVPMIRF
                     SDVGDKVLFIENKFKMSDNYFTSHILMIPEMSSLKEILVRLGLEV"
     gene            2396..2881
                     /locus_tag="KPD58_001949"
     CDS             2396..2881
                     /locus_tag="KPD58_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HBG0265715.1"
                     /translation="MNREIVVNIADMKIAYRPNVLVTYALGSCVGVCLIDKVAGIGGM
                     LHVMLPYSKDAINIENKCKFADTGINELIKSMENIGANRIYIKAKIAGGAQMFLGSSN
                     SVIASIGHRNVEAVKKTLLGLNIPILAEDTGMNYGRTIRFYTETGELLVDSINKGKKK
                     L"
     gene            2924..3442
                     /locus_tag="KPD58_001950"
     CDS             2924..3442
                     /locus_tag="KPD58_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-binding chemotaxis protein CheW"
                     /protein_id="HBG0265716.1"
                     /translation="MRFPDDPYLDYGLIDEGIKYLKFKVDEQDFGVELEKIIEITGNQ
                     EIIFIPELPTYSKGIINLRGKIIPIIDMRLRLKKDELMTTNCMYIVVTEIKDLVVGFV
                     VDRVDDIVSLEKSKISPPPRVTIEYSDYFVSGVGELEGKIITLLDLEKLLTDKDEVLI
                     DELINGFDCTIM"
     gene            3578..4537
                     /locus_tag="KPD58_001951"
     CDS             3578..4537
                     /locus_tag="KPD58_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG0265717.1"
                     /translation="MKNKQQGTILVVDDSVLMCDLIDKTLTKDNFKVKKAFNAIEAKE
                     FIEEFIPDIILLDIILPDNSGFEFCKEVKSNARTADIPIIFITSKNTDTDIVKGFGVG
                     AIDYMVKPFSMTELKARVIAHLEVKKSQDKLKKINNELESSLEKLNRLVVRDYLTGLY
                     NRRHIINQLMEQRRINKYSETSISLILGDIDDFKVINDTYGHEAGDFVLTVISSIIKK
                     NCRQIDTVSRWGGEEFLIMLTNTPIEGAKILSERIRKEIKEFDFNYKEHKIKCTITLG
                     VVEVNSNISLEENISLADKAMYEGKNLGKDCSVVSTMKGLKKI"
     gene            4563..6266
                     /locus_tag="KPD58_001952"
     CDS             4563..6266
                     /locus_tag="KPD58_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HBG0265718.1"
                     /translation="MKREVKLSKLAIVLLIIIVLAVLAGIFSSISSYKAFEKVEKSIN
                     DMNNLRTQSQYIKDTTREISDVLRRYVFTGEKKNKDIYEKELYGKTRESALNKLEKIG
                     LTKSESNKILEAKEISDSLVPTEQSAIKAVESNDKSKAQSIIFGSDYINSQEKMDSLI
                     NEFQEDIVTRSNSKIVEVKKELQSLILRTIFILSLLIILTIIEYTVIKLKIVIPINKV
                     EKHFSRIASGDLRTSIDVEESKSEIGLLVSSVKKMQSMLLNYIDLIDSTLTSIAKGDL
                     RVEIDQEFIGDFKSIKISLESIINSFNGDFSEINLSAEQVASASEQVAAGAQALSQGA
                     TEQASSVEELASTINEISDGIKKCADSASLACEVSDQSASQVGFGSRCMDDMMTSMGE
                     ISEKSSEISKIIKTIDEIAFQTNILALNAAVEAARAGQYGRGFAVVADEVRSLAGKSA
                     KAAQNTAILIEEAIKTVENGVRIAKETANALDLVVNGVEKTTNYINGISSAVHSQEVA
                     ITQILSGIEQISIVVQMNSATAEESAAASEEMSAQAQILKELVEKFHLKESEVRESSW
                     V"
     gene            6479..8581
                     /locus_tag="KPD58_001953"
     CDS             6479..8581
                     /locus_tag="KPD58_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HBG0265719.1"
                     /translation="MRQMLDMFIFETSLLLEQLDEIMIQIEKEKVFTHNNINETFRIM
                     HTIKGSASMMGLDNISALAHSVEDLFYAIREGKIEVLDNDLLFDLVFQSLDFIRRDID
                     ELQSRGCISSDYSNFIEGIEEYVSTLELSSSMKSKDNANLESTDEKEVIDSNFEYKEG
                     SDVVKIIFEQGCMMENVRAFMLVFELRDYCEFIDFYPKDVETNAESTEYIKSYGFYIQ
                     FVAKEDKAIVYKTIEDNLYVKEYINISKKEIEMKNTSNNDAVENKLKDNNKVSKSYQN
                     NYTEDEIQEDVLNEKTDMEKDSLSEENEHLPISKQSIMSVNLEKLDLLQNIVGEIVIM
                     ESMVVNSVNSTGVDIDNFNKATRQLHKLTDELQDIVMSMRMIQIAGVFQKMHRIVRDM
                     NKKLDKNVELITIGAETEVDKSVIDNLAEPFMHLIRNAMDHAIEPIEERIAKGKEEVG
                     KIVLSAENLGGEAVISISDDGRGIMRDKILAKAKEQGLLKHQSNEYTDDEVNSLIMTP
                     GFSTKESITEFSGRGVGMDIVRKSIEKVGGSIEVKSKQDEGTTFTIKIPLTLSIVEGM
                     KFKVGDSLFTLPISSIRRTFKLTDLNQIITEGNKTELITIYGACIPIIRLHKIYNIQT
                     KVTNLMNGNMIIIENEHKAVCIFVDEIIGNQQVVVKPFPVYFNRFNIKNKGLSGCTIL
                     GDGSISLIIDADSIIEIY"
     gene            8594..9034
                     /locus_tag="KPD58_001954"
     CDS             8594..9034
                     /locus_tag="KPD58_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HBG0265720.1"
                     /translation="MAKDEIIKQVLTFYVNDVIYGIELENVIETIRFQSITYVPCLPT
                     YISGVINLRGRIIPVINMHIKYNLPKADYNERTCIIITKVDEYQVGIIVDKVVDVIHI
                     ENLNLLETTNSNNTNINKDIKEIAKVEDNSILILDIRKFLINSI"
     gene            9070..9873
                     /locus_tag="KPD58_001955"
     CDS             9070..9873
                     /locus_tag="KPD58_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HBG0265721.1"
                     /translation="MIKLTDEEFIVLVNHVKKEYGIDLSKKRALIEGRLYNTMIEKKL
                     SSFSQYMNLLFKDKTGNEAINLINRLSTNHTFFMREPQHFEFIQNSILPFWEENNKIR
                     NINIWSAGCSSGEEAYSIAMTLDDYFGYNKELWNIKIIATDISTNSLEKAKRGIYIES
                     NVNNVPELWKKKYFIDNKDGTFKICDKIRKSVIFKPFNLMNDFSYQHFDLIFCRNVMI
                     YFDLKTREELINKFYNATKKDSFLFVGHAEVINRSSTKYKYVKPAIYKR"
     gene            9913..10509
                     /locus_tag="KPD58_001956"
     CDS             9913..10509
                     /locus_tag="KPD58_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HBG0265722.1"
                     /translation="MERFNNMVIAIGASVGGTEAILEIIKDLPKSTPGIVVVQHMPAI
                     FTYMYAQRLDKQCIMNVREAKNNDRVEQGNVLIAPGGYQMKLCTDKQGYYVTCEKGER
                     ISGHCPSVDVLFDSVAEVAGKNSIGIILTGMGYDGASGLLKMKENGSFTIGQDENSCI
                     VYGMPMVAFDRGSVMLQLPLGEISNCLIRHINILKQKD"
     gene            10615..11478
                     /locus_tag="KPD58_001957"
     CDS             10615..11478
                     /locus_tag="KPD58_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HBG0265723.1"
                     /translation="MKFFKVDTKAKVLRYVFVLTLTIMAVLAIKHDYKYNNLRKDASI
                     NLNSNKEAVTVMSNQSLPKGNTNSKQTLSVSTIGSKTIVKNDEYLESTINMPVITNSN
                     KIVERSTNDKIKNDIFEFYNKSYKEAKQYLKDNPDEKNKFVANVDFELKKNTDSALSI
                     KVRYYTYSGGAHGFYQDIAYNVDMRTGKFLELMDLFKDNTKYKEVIDEEIKRQIAELE
                     KKDEENIGIYNFKGIKENQNFYLQDENLVVYFDLYDITPYAAGIPEFSINKALISSML
                     KPEYVDLFDLK"
     gene            11545..12534
                     /locus_tag="KPD58_001958"
     CDS             11545..12534
                     /locus_tag="KPD58_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBG0265724.1"
                     /translation="MLLLKKDDIKKVFTMRDAIEADKEAFRIYCEGKSVNPLRTNISV
                     PSQDASMLFMPGYVEELGCGGIKIVSVFPKNAQKGKPVIPATVLLLDGETGEVSAVLD
                     GTYVTQIRTGAASGAAIDVLAKKDSKIGALIGLGGQGEPQLEAMIEARNLDEVRVFSR
                     NKESREKFAEEMNLKLSKYNTKIVAVESSDEAIDNADVIVLATPSKQPVLNGNLVKKG
                     ALISAVGSYMPDMQELCPNCLTRASKIFFESTDAVLSESGDILIPLKEGKISKDDFSG
                     DLGNVINGTIPGREDDDEIIVFKTVGIGVQDVVTAKRIYDKAKENGIGIDWKW"
     gene            12626..13237
                     /locus_tag="KPD58_001959"
     CDS             12626..13237
                     /locus_tag="KPD58_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG0265725.1"
                     /translation="MKMVKKLILSTIMALVLVLTIGCSNAEEKVKSNFEANLAALEMY
                     KYDEKGISDALTEAEYKQIQTYNSKDKVSMIAQLTPLYGKENAEKIYETIVKLIQGVE
                     KTVTVESSSKDKITLKVVSKALDTSKVMSEANTLVKSYVTDEKNYDKKEKEVQDDMTK
                     MIIDLYNKKPTKEVTTMITYTKKDGKWQPPSNIQKLCEGIYTI"
     gene            13698..14477
                     /locus_tag="KPD58_001960"
     CDS             13698..14477
                     /locus_tag="KPD58_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBG0265726.1"
                     /translation="MVSRQELKRVSKAQLSGNWGICAISMAVYIALIVLISIVGLPFE
                     PLTTIVVAVIAFCVSAGFTCMFLKITKDEKVKIGDIFVSGRIYLRSFGFYLFESIIVY
                     IYSFIITIIGAFLSFGVLLTSGAIEYLSSDNVGIGTIISTGITLVLIILVLMLPVYIL
                     LLYVSQATFLICEDKDNMGVFKSMGASLDMMKGNVLKLFVLNFSFIGWYILSIATLGI
                     GLLWLIPYVGVTNCNFYRQLVKENPDVEDILLDKEKSYTMY"
BASE COUNT         5854 a         1385 c         2670 g         4672 t
ORIGIN      
        1 tcactgagtt taattatatt aaaaatatga cataaaggtg tcgtatatta taaattataa
       61 ttgaattagt tcaaaataaa atagattaat aaaaacaaag tgaggtataa agtatgaatt
      121 atgaaatagt agagataaat gagaaaatag ttataggaat atcgaaggag actacaaata
      181 aagatggaca agcggtaaat gatataggtg aattgtggaa aaaatttatg ggcaaaggga
      241 tatataatgc tataaagggt aaaaagaatg acaagacaat aggtctatat acagattacc
      301 agggtgattt tacaagtccg tatagctttg ttgcatgttg tgaagtaaat agtaattctg
      361 ataaagagaa gaatttagaa gaattaaaca ctaataatac tgttggagaa agtattatta
      421 gtaaagttat accagcaggt aagtatgcaa agtttgttat agtaggaggt caaaaagaag
      481 taggagattt ttggtttgag ttttggcaaa tggattttga taggacttat ataagtgatt
      541 ttgaagagta tcaatgtaat acttttgata caaagaaaca agaaattcat atttacatag
      601 gaataaagta gatttgttat tactatattg aatgaaatat tgagtatagt acatttatta
      661 tagatagttt tattatttat aactataagc agtaaatctg taaaagattt actgcttttt
      721 tagtaattaa tagtatcagt atcactttga atcttcagac atattcttca tttaatattg
      781 tttattgata aatgatactt gtaaatataa tgatttgtta tttaatttaa aatatgaaaa
      841 aaagtattat aaacacatta gaatacttct ggatttatga gtatacaatt tgaaaaatat
      901 aaaaaataat ttataaagct aaatctaatt taatcgataa tacatataga gaaatataat
      961 taagtaaata aacaactatc tattagatat tgagaaagtc aaatcctata tgaatctaca
     1021 gaaatgattt taatgaatct gaaaattttt tttatacaaa cataaaaaag gtataaagtt
     1081 gttttattat aatttaatta cttttgataa ctttttatgt tttatacaat tatattttta
     1141 ataactcgtc aaaacgccaa acatatatta caatagatat ataagttttt ttgttgacaa
     1201 attatgttaa gagaattaag tataatatgg cataaataat tacttttatt tatcaatgaa
     1261 atgtatttat tgtgtagcga aaaatagtca gatagtatag acaataactg aagataaaag
     1321 cgtttattgt tgtacatatg gaggagttag ttttatggaa aataaaattt tgatagtaga
     1381 tgatgctatt tttatgagaa tgataattaa agaagctctg agtaaaagtg gctataataa
     1441 tttaatagaa gcaaatgatg gagaagaagc gtgtaatata tttaaatatg aaaagcctga
     1501 tttgatgttg cttgatatta caatgccaaa aaaagatggt ttagaggtgc taagagaaat
     1561 aaaaaagttg gattcaagtg ctaaggttgt aatgtgctct gcaataggac aagagaatat
     1621 gattgtagaa gccataaaac ttggagcgtt agattttata gtgaagccct tcaaaacaga
     1681 aacattagtt aaagttgtag ataatgcttt aaaataaaag tgtaaatata cactaataca
     1741 atgaaaagca aataaatgca ataataaagg gaggggttta tattataaat tatttagaac
     1801 ttgatgatgt tcatatagat gctcttagag aaattggtaa tataggctct gggaatgcaa
     1861 ttactgcact tgcatccatg ataaatagca atgtagaagt attaatacct atggtcaaga
     1921 tacttgaata taatgaagcg acaaatttgc ttggtggtcc agaaaacaaa gtagtatgta
     1981 ttttactcga tatgaaaggt gatatcaatg gaatgtttat gtttttgttg gatgaatcaa
     2041 taactcaact tatgctaagt tctcttttta ataaagaaga agctttttta gatgagattg
     2101 aggcaattga aatttcagca attaaggaaa taggaaatat tatggcaagt tcatatgtaa
     2161 atgctattgc atctatgtta aacatgacta taagtgtttc aattccagat atatgtattg
     2221 atatggttgg agctgttcta aatgtaccta tgataagatt ttcagatgtt ggtgacaaag
     2281 tcttatttat agaaaataaa tttaaaatga gtgataatta ttttactagt cacattttga
     2341 tgattccaga aatgagctca cttaaagaga tactcgtgag gttgggatta gaggtatgaa
     2401 tagagagata gtagtgaaca ttgcagatat gaagatagcc tatagaccaa atgttttagt
     2461 tacttatgca cttggttctt gtgtaggagt atgtttgata gataaagtag ctggaattgg
     2521 tggaatgtta cacgttatgt tgccatatag taaagatgct attaacattg aaaataagtg
     2581 taaatttgct gacacaggaa taaatgagct cattaaatct atggagaata taggtgcaaa
     2641 tagaatatat ataaaagcaa aaattgctgg aggtgctcag atgtttttag gaagtagcaa
     2701 ttcagtaatt gcaagtatag gacataggaa tgttgaggca gtaaaaaaaa ctcttttagg
     2761 attaaatatt ccaatattag ctgaggatac aggtatgaat tatggtagaa caatacgttt
     2821 ttacactgag acgggtgaat tacttgtaga ttcaataaat aaaggtaaga agaaattata
     2881 atatatattt catagaaagt gtattttagg agggaggtat taaatgagat ttccagatga
     2941 cccatattta gattatggac taattgatga aggaattaag tatctaaagt ttaaagtaga
     3001 tgagcaagat tttggggttg aacttgaaaa gataatagaa attactggaa atcaagaaat
     3061 tatatttatt ccagaactac ctacatattc taaaggcata ataaatctaa gaggaaaaat
     3121 cattccgata atagatatgc gtttgagact taaaaaagat gaattaatga ctacaaattg
     3181 catgtatata gtagtaactg aaataaagga cttggtagtt ggatttgtgg ttgatagagt
     3241 tgatgatata gtaagcttag aaaaaagtaa aatatcacct ccaccaagag ttactataga
     3301 atattcggat tattttgtaa gtggagttgg agaattagaa ggtaaaataa ttactttact
     3361 agatttagag aaacttttga cagataaaga tgaagtttta atagatgaat taatcaatgg
     3421 gtttgattgc actatcatgt aatataggga tgtagttgga gtgttattat aaaaagattg
     3481 tgatgagaac atttaagaat atagtggtat aattattaag tataaaaatt ttaaatcatt
     3541 aaaatttgaa tatattatgt ttaagagagg tttgtatatg aaaaataagc aacagggaac
     3601 aattttagtc gttgatgata gtgttctgat gtgtgactta atagataaaa cacttacaaa
     3661 agataatttt aaggttaaaa aagcatttaa tgcaatagag gcaaaagaat ttattgaaga
     3721 atttatacca gacattattt tgcttgatat aattttacca gataattctg gttttgagtt
     3781 ttgtaaggaa gtaaaatcta atgctcgtac agcagatata cctataattt ttataacttc
     3841 aaaaaataca gatacagaca tagtaaaagg atttggtgta ggagcaattg actatatggt
     3901 gaaacctttt agtatgacag aattaaaagc tcgtgttata gctcatcttg aagtaaagaa
     3961 atctcaagat aaactaaaaa agataaataa tgaactggaa tcttctcttg aaaaattgaa
     4021 tagactcgtt gtaagagatt atcttacagg tctttacaat aggcgacata taattaatca
     4081 gcttatggag caaagaagaa ttaataaata tagtgaaaca tcaatatccc ttatccttgg
     4141 tgatattgat gactttaagg ttatcaatga tacttatggg catgaagcag gggatttcgt
     4201 gttaacagta atatcaagta taataaaaaa gaattgtaga caaattgata ctgtatcaag
     4261 atggggtgga gaagagtttt tgataatgct cacaaatact ccaatagaag gtgctaagat
     4321 actttcagaa agaatccgta aagagataaa ggagtttgat ttcaactata aagaacataa
     4381 aataaagtgc actattacat taggagtagt agaagtaaac tcaaatatat cattagaaga
     4441 aaacatatcc ttagcagata aagcaatgta tgagggtaaa aacttaggaa aagattgtag
     4501 cgtagtgagt acaatgaaag gattaaaaaa gatataaact tactttatga aggagtacag
     4561 atatgaaaag agaagtaaag ctttctaaat tagcaattgt attgctgatt attatagtat
     4621 tggctgttct agcagggatt ttttcttcta tttcatctta taaagcattt gaaaaagtag
     4681 aaaaaagtat taatgatatg aataatttga ggactcaaag tcagtatata aaagatacaa
     4741 ctagagagat aagtgatgta ttaagaagat atgtatttac aggagaaaaa aagaataaag
     4801 atatttatga aaaagaatta tacggaaaaa caagagaatc agctttaaac aaattagaaa
     4861 aaattggtct tacaaagagc gagtcaaata aaatcttaga agcaaaagaa atatcagatt
     4921 ctttagtacc aacagaacaa agtgcaataa aggcagtcga aagtaatgat aagagtaaag
     4981 ctcaaagtat aatttttggt agtgactata taaactccca agagaaaatg gattctttaa
     5041 taaatgaatt tcaagaggat atagttaccc gttcaaattc caaaattgta gaagtaaaaa
     5101 aagaattaca aagtctaata ttaagaacaa tatttatact ttccctttta ataatattga
     5161 caataataga atatacagta ataaaattaa aaatagtgat tcctattaat aaagtagaaa
     5221 aacacttttc aagaattgca tctggggact taagaacaag tatagacgtt gaagaaagta
     5281 aatctgaaat aggactatta gtgagttctg tcaaaaagat gcaaagtatg cttttaaatt
     5341 acatagacct tatagacagt acattaacta gtattgcaaa aggagattta agagtagaga
     5401 tagaccaaga atttataggt gattttaaat caattaaaat atctttagaa agtataataa
     5461 actcttttaa tggtgatttt tctgaaataa atttatcagc agagcaagtt gcaagtgctt
     5521 cagaacaagt tgcagcaggt gcacaagctc tttcacaggg tgctactgaa caagctagtt
     5581 cagtagaaga attagcttcc acgattaatg agatatcaga tggaattaag aagtgtgcag
     5641 atagtgctag tcttgcatgt gaggtatctg accaatctgc atcacaagtt ggtttcggaa
     5701 gtagatgtat ggatgatatg atgacatcaa tgggtgaaat aagtgaaaaa tcgagtgaaa
     5761 tatcaaaaat aatcaaaaca atagatgaga tagcattcca aactaatatt cttgcattaa
     5821 atgcagctgt tgaagcagca agagcaggtc aatatggtag aggatttgca gttgtagcag
     5881 atgaagttag gtctttggct ggtaaaagtg caaaagcagc tcaaaataca gctattctca
     5941 tagaagaagc tattaaaaca gtagaaaatg gtgtcagaat tgctaaagag actgcaaatg
     6001 cattggactt ggtagtaaat ggtgtagaaa aaactactaa ttatattaat ggtatttctt
     6061 cggctgtaca tagtcaagaa gtagcaatta cacagatttt atctggaata gagcaaattt
     6121 ccatagtagt ccaaatgaac tcagcaacag cagaggaaag tgcagcagcc tcagaagaga
     6181 tgtctgctca agctcaaatt ttaaaagaat tagtagaaaa atttcatcta aaggaaagtg
     6241 aagtaagaga atcatcatgg gtttaaatta tgaggtgaat attgaagtaa gagaattatc
     6301 atgtatttaa attatgagat gtatatatat tgaagtaaga gaataattgt gtatttaaat
     6361 tatgaggtac atatagtgag ataagagaat catcaaggat ttaaattatg agatgcatat
     6421 ataatagaaa attatttgta ggtcgtaggt gataaatgtg aattatattg aaccgagtat
     6481 gagacaaatg ttagatatgt ttatatttga gacaagttta ttactcgaac aattagatga
     6541 aattatgatt caaatagaaa aagagaaagt atttactcat aacaacatta atgaaacttt
     6601 tagaataatg catacgataa aaggttcagc atctatgatg gggttagaca atatatctgc
     6661 tttagcacat agtgtagaag acttgtttta tgctatacgt gaaggtaaaa tagaagtttt
     6721 agacaatgac ctattatttg atttagtctt tcaatcatta gattttataa gaagagatat
     6781 agatgagtta caatcaagag gttgtatatc ttctgattac tctaatttta ttgaaggaat
     6841 tgaagagtat gtgagtactt tggaattatc aagttctatg aaatcaaagg ataatgcaaa
     6901 tttagaaagt acagacgaaa aagaagtaat agattcaaat tttgaataca aagaaggctc
     6961 tgatgtagta aaaataatct ttgaacaagg ttgcatgatg gaaaatgtga gagctttcat
     7021 gcttgtattt gaacttagag attactgtga atttatagat ttttatccaa aagatgtaga
     7081 aacaaatgca gagtctacag agtatattaa aagttatggg ttttatatac aatttgtagc
     7141 taaagaggat aaggcaatag tttataaaac tatagaagat aatctctatg taaaagaata
     7201 cataaatatt agtaaaaaag aaatagaaat gaaaaataca tcaaacaatg atgcagtaga
     7261 aaataaatta aaagataata ataaagtaag taaaagttat caaaataact atacagaaga
     7321 tgagatacaa gaagatgttt taaatgaaaa aactgatatg gaaaaagata gtctaagtga
     7381 agaaaatgaa catttaccaa taagtaagca gagtattatg agtgtaaatt tagaaaaatt
     7441 ggacttactt caaaatatag ttggagaaat tgtaattatg gaatcaatgg ttgtaaatag
     7501 cgtaaattct acaggtgttg atatagataa tttcaataag gcaacaagac aattacacaa
     7561 gcttacagat gaattgcaag atatagtaat gtctatgcgt atgattcaaa ttgctggtgt
     7621 atttcaaaaa atgcatagaa ttgtacgaga tatgaataaa aaattagata aaaatgtaga
     7681 gttaataact ataggagcag agacagaggt agataagagt gtaattgata atttagcaga
     7741 accttttatg caccttataa gaaatgctat ggaccatgca atagagccta ttgaggagcg
     7801 tatagcgaaa ggtaaagaag aagtgggaaa aatagtttta tctgctgaaa atctaggcgg
     7861 agaagctgtg ataagtattt cagatgatgg tagaggcatt atgagagata agattttggc
     7921 aaaagctaag gaacaaggtc ttcttaaaca tcaaagtaat gagtatactg atgacgaggt
     7981 caattctttg attatgactc caggcttttc tacaaaagaa tctataactg aattttctgg
     8041 aagaggagta ggcatggaca ttgttcgtaa aagtattgaa aaagttggag gttcaattga
     8101 agtaaaaagt aaacaggatg aagggacaac ctttactata aaaataccat taactctttc
     8161 tatagtagag ggtatgaagt ttaaagttgg agattcacta tttactttac caatatcatc
     8221 cataagaaga acatttaaac ttacagactt aaatcaaata attacagaag gaaataagac
     8281 agagttaatt acaatatatg gagcatgtat tcccattata aggttacata aaatatataa
     8341 tattcaaaca aaagtaacca atctgatgaa tggtaatatg ataattattg aaaatgagca
     8401 taaagcagta tgcatttttg ttgatgaaat aataggtaat cagcaagttg ttgtgaaacc
     8461 atttccagtt tattttaacc gatttaatat aaaaaataag ggtttatcgg gatgtacaat
     8521 acttggggat ggaagtataa gtttaattat agatgcagat agcattatag aaatatatta
     8581 ggggatttaa agtatggcta aagatgaaat tattaaacaa gtattaacat tttatgtcaa
     8641 tgatgttatt tatggaatag aattagaaaa tgtgattgaa accataagat ttcaatcaat
     8701 aacgtatgta ccatgtcttc caacatatat aagtggagtt attaatttga gaggaagaat
     8761 aataccagtt attaatatgc atataaagta caatcttcct aaagctgatt ataatgagag
     8821 aacttgtata ataattacaa aggttgatga gtatcaagtt ggaattattg tagataaagt
     8881 tgttgatgtt attcatatag aaaatctaaa tttactagag acgactaatt ctaacaacac
     8941 taatattaat aaagatataa aagagattgc gaaagttgaa gataattcta tattaatttt
     9001 agatattcga aagtttttga ttaatagcat atagttagtt ctgataaatt agcaggagga
     9061 agtttttgca tgataaaact cacagatgaa gaatttatag ttcttgtaaa ccatgtaaaa
     9121 aaagaatatg gtattgattt aagtaaaaaa agagcgttaa ttgaaggtcg cttatacaat
     9181 acaatgatag agaaaaaact tagttctttt agtcaatata tgaatctatt atttaaagat
     9241 aagacaggaa atgaagctat aaacctaata aatagattga gtactaacca cacatttttt
     9301 atgagagaac ctcaacattt tgagtttata cagaatagta tacttccttt ttgggaagaa
     9361 aataataaaa taagaaacat aaatatatgg agtgcaggat gttctagtgg agaagaagcg
     9421 tattcaatag ctatgacttt ggatgattat tttggatata ataaagagtt gtggaacatt
     9481 aaaatcatag ccacagatat ttcaactaac tcacttgaaa aagctaaaag aggtatatac
     9541 atagaatcaa atgttaacaa tgttcctgag ttatggaaga agaagtattt tatagacaac
     9601 aaggatggta catttaaaat ttgtgataag attagaaaaa gtgtaatttt taagccgttt
     9661 aatttaatga atgatttctc atatcaacat tttgatttga ttttttgtag aaatgttatg
     9721 atttattttg atttaaaaac aagagaagaa cttataaata aattttataa tgctaccaaa
     9781 aaagatagtt ttttgtttgt aggacatgca gaagtaatca acagaagtag tactaaatac
     9841 aaatatgtaa aacctgctat atataaacga taaaaaggta tttttgatat atgatttttt
     9901 ggaggcggta atgtggaacg ttttaataat atggttattg ctataggcgc atctgtaggt
     9961 ggtacagagg caatacttga aattataaaa gacctaccaa aatcaacacc agggattgtt
    10021 gtagttcaac atatgccagc tatatttact tatatgtatg cacaacggct tgacaagcag
    10081 tgtattatga atgtaagaga agctaagaat aatgatagag tagaacaagg aaatgtgtta
    10141 attgcacctg ggggttacca gatgaaattg tgtacggata aacaaggata ttatgtaact
    10201 tgtgaaaaag gagaaagaat aagtggtcac tgtccatcag tagacgtttt gtttgattca
    10261 gtagcagaag tagcaggtaa aaattcaata ggaataatac ttacaggtat gggttatgat
    10321 ggagcaagtg gacttttaaa aatgaaagaa aatggttcat ttacaattgg acaggatgaa
    10381 aattcttgta tagtatatgg aatgccaatg gtagcttttg atagaggttc tgttatgtta
    10441 caattacctc ttggtgaaat ttcaaactgt ttgataagac atattaatat tttaaaacaa
    10501 aaagattaat tttttataaa taaaaattaa tcttttatta aattatatgg ataaaaaacc
    10561 ctagaaactc caaatataat aagagagatt tatatttagg aggaaaatga taatatgaaa
    10621 ttttttaaag tcgatacaaa agctaaagtt ttaagatatg tatttgtttt aacattgact
    10681 ataatggctg ttttggcaat taaacatgac tataaatata ataatttaag gaaagatgct
    10741 tcaattaatt taaatagtaa taaggaagca gtgactgtta tgagtaacca gtcattacca
    10801 aagggtaata caaatagtaa acaaacttta tcagtaagta caataggaag taaaacaata
    10861 gtgaaaaatg atgagtattt agaaagtact ataaacatgc ctgtaataac aaattcaaat
    10921 aaaattgttg aaaggtctac aaatgataag ataaaaaatg acatatttga attttataat
    10981 aaatcatata aagaagcaaa gcagtatctt aaggataatc ctgacgagaa aaataagttt
    11041 gttgcaaatg tagactttga acttaaaaag aatactgatt ctgctcttag tataaaagtg
    11101 agatattata cttatagtgg tggagcacat ggattttatc aagatatagc atataatgtg
    11161 gatatgagaa ctggaaaatt tttagaatta atggacttgt ttaaggataa tactaaatat
    11221 aaagaagtta tagatgaaga aattaaaaga caaatagctg aacttgaaaa aaaggatgaa
    11281 gaaaatatcg gaatatataa ttttaaaggt ataaaagaga atcaaaactt ttacttgcaa
    11341 gatgagaatc tagttgtata ttttgattta tatgatataa ctccatatgc agcaggaata
    11401 ccagaatttt caataaataa agcgttgata agttctatgc taaaaccaga gtatgtagat
    11461 ttatttgatt tgaaatagaa gattaaatta aatgttatgc tataatattt agcatagaga
    11521 cttattaaaa ttgggggtat aaaaatgcta cttttaaaaa aagacgacat aaaaaaagta
    11581 tttacaatga gagatgcaat tgaagctgat aaggaagctt ttagaattta ttgtgaagga
    11641 aagagtgtaa atcctttgag aaccaatata tctgttccaa gtcaagatgc tagtatgtta
    11701 tttatgccag gatatgtaga agaattaggc tgtgggggaa taaaaattgt atctgtattt
    11761 cctaaaaatg ctcaaaaagg caaaccagta attccagcaa cagtactatt attagatgga
    11821 gaaactggag aagttagtgc agtattggat ggtacttatg taactcagat tcgtactgga
    11881 gcagcctctg gagcagctat agatgtattg gctaaaaaag attctaagat tggagctttg
    11941 ataggtctag gaggacaagg tgagccacaa cttgaagcca tgatagaagc tagaaatttg
    12001 gatgaggtaa gagtttttag tagaaacaag gaaagtagag aaaaatttgc agaagaaatg
    12061 aacttaaaat taagtaaata taatactaaa atagttgcag ttgaatcatc tgatgaagcc
    12121 atagataacg cagatgtgat agttttagct actccatcaa aacaaccagt acttaatgga
    12181 aacttagtga aaaaaggagc tttaataagt gcagtaggtt catatatgcc agatatgcaa
    12241 gagttgtgcc caaactgttt aactagagcg tctaagatat tttttgaatc aacagatgca
    12301 gttttatctg agtcaggaga tatattaatt ccattaaaag aaggtaagat aagtaaagat
    12361 gattttagtg gtgatttagg aaatgttata aacggaacta ttcctggaag agaagatgat
    12421 gatgagataa ttgtatttaa aactgttggg ataggtgtac aagatgtcgt aacagcaaaa
    12481 agaatatacg ataaagcaaa agaaaacggt attggaatag actggaagtg gtaaaatatt
    12541 caaaataatt ctatagttag acaaatttat gttataatgt tgataaataa attttttatt
    12601 attttaattg agggaggatg tttgtatgaa aatggtaaaa aagttaatat tatctacgat
    12661 tatggctcta gttttagttc taactatagg gtgttctaat gcagaagaaa aagtaaagtc
    12721 gaattttgaa gctaatttag ctgcattaga aatgtataag tatgatgaaa aaggaatttc
    12781 tgatgcatta acagaagctg aatataaaca gattcaaact tataacagca aggacaaggt
    12841 ttcaatgatt gctcaattga cacctcttta tggtaaagaa aatgctgaaa agatatacga
    12901 aacaattgtt aaattaattc aaggtgttga aaagacagta acagtggaat cttctagtaa
    12961 ggataagata actttgaaag ttgtaagtaa agctttagat actagtaaag taatgagtga
    13021 agcgaacact ttagtaaaat cttatgtaac tgatgaaaag aattacgata aaaaagaaaa
    13081 agaagttcaa gatgatatga caaaaatgat tatagacctt tacaataaga aaccaactaa
    13141 ggaagttaca actatgatta catatactaa aaaagatggt aaatggcaac cacctagtaa
    13201 tatacaaaag ttatgtgaag gaatatatac aatttaattt attttaaaga cacaaaacta
    13261 tcttttaagc atataataaa atgtttggaa gatagtttat tttattttca aagtataaat
    13321 tatattttat aaatgattta ttttataaat aatttatctt gataaatagt aaataattta
    13381 ctttactaat aataaataat ttatcttacc aattaattta ctgtattagg taattttttt
    13441 attaaataat ttgtttacta aataaagtat aattaataga aattatttta ttacttgttg
    13501 aataattaat caaatttatt tgatgtattt ggtaatgttg actatataaa actagtaata
    13561 caattatggc atatcttgat aaaacatggt gaataattgt atcttatata gtcgaaaata
    13621 tgatatatta atattatacg ttaaaagatt atttatattt ttaaaagttt ttttataaaa
    13681 tgaaaggggt gttttttatg gtatcaaggc aagaattaaa acgagtatct aaagctcagt
    13741 tatctggaaa ctgggggata tgtgcaattt ctatggcagt atatattgca ttgatagtat
    13801 taatatcaat tgtaggatta ccttttgaac cattgactac tattgtagtg gctgtaatag
    13861 ctttttgcgt ttcagcagga tttacatgta tgttcttgaa aattactaaa gatgaaaaag
    13921 tcaagatagg agatatcttt gtaagtggga gaatttattt aagaagtttt ggtttttatt
    13981 tatttgaatc aattattgtt tatatttatt cttttataat cacaattata ggagcgtttt
    14041 tatcatttgg agttttactt actagtggag caattgaata tttatcaagt gataatgttg
    14101 gaatagggac tattatttct actggaataa ctctagttct tataatactt gttcttatgt
    14161 taccagttta tatattgcta ttatatgttt ctcaagctac tttcttaatt tgtgaagaca
    14221 aggataatat gggtgtattt aaatcaatgg gtgctagttt agatatgatg aaaggaaatg
    14281 tacttaaatt atttgtacta aactttagtt ttataggatg gtatatactt tcaatagcta
    14341 ctcttggaat aggtctacta tggttaattc catatgtggg tgttactaat tgtaattttt
    14401 atagacaatt agtaaaagaa aacccagatg tagaggatat actattagat aaagaaaaat
    14461 cttatactat gtactaagat agatagttgt atatttaaat aaaatatggc tttaattaaa
    14521 cattaaagta taacattgat aaaactgtct tttataagaa ttttttaaaa agacagtttt
    14581 t
//
LOCUS       DAELLV010000083        14498 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.083, whole genome shotgun sequence.
ACCESSION   DAELLV010000083 DAELLV010000000
VERSION     DAELLV010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14498
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.083"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1501
                     /locus_tag="KPD58_001961"
     CDS             <1..1501
                     /locus_tag="KPD58_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265727.1"
                     /translation="QNPIQEYYEFLEDIWKNNIKIDELINKYLITATRMDQIKFPLIY
                     IDEDSFCPYCLGNKFNIINNIKQQDIKVECEKCGEAISYFIHKEEVDNIIEEEKRKKE
                     LFIRTLENVYNKINKIKCHRCQSDLYVCINDKIMEYNIRCDRCNSQYESVEIAQEQYR
                     LWQQRAAMMANIKNKENELLLKTLSIKDEKDIKFKLEDIITESESIDVISDLVDMQDR
                     GENIFKQLFNNYVRLGRLDRRLLVTVCKDCKQNENTYTWERGKEHLINMRGITYSEAV
                     VYELMEKTKLLPIRAIIRRLMEKKLLYCDERSNNLQVHPVLIENLAKLESINKSQNIN
                     NQVAYLIFNKQKYICYHCGESGRPLKIGYLTIDKNINDLSSLVGICDSCYEDITEDEI
                     IIDNIFCVVDERNNELGEAWKFLIQYDSSFRNNTIAEVEHARLYDMYDEDNLIRAYAN
                     TLYKQTKDGFANEKVFFAYARAILKNSKGQVSVLQIAKEKFKLEKWLNG"
     gene            1733..3205
                     /locus_tag="KPD58_001962"
     CDS             1733..3205
                     /locus_tag="KPD58_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265728.1"
                     /translation="MSHSKLSDHKLKKGKFIAPFNEFMTPLNSNESWCLGRLPEYIWI
                     GLLINKYGRKKGMEIIYKIIVKLHSIESDIVTPRLSEILSMEDVKQDEFFSYILTIVE
                     KEALSPLTLIFTYSNYPIFVKYFAEPSIKIEDRVTYLDETLRKAMHHQSEFATDIRFI
                     VLYYFCMSGKLVLQKEQINNLLEYPNLEHTDEKMRMLRPFIRAAELMVLNMEKKSDWY
                     LDIFWGRISVMTECKLYATKFEDETANTDLYMEQLHEIFVYLSDLLCKVNPLDEKMLV
                     LLGIAIYSYKRLREVVEHKLFNSISGRGSVRVLIENYIMMKYLVKIEAQHENIWSEYQ
                     FYGIGQYKLIVARYREQEQDLKNSHVDYKYIEALVNEFVIEETIDMDTKYFDKQNIRS
                     KAEIVGEKELYGLYYDYDSAYEHGLWGSIRESSMLKCDNPAHHYHCIPDVDNNQNLKS
                     VWHDCVNIMEKTLLFLDTVYGIPQHLLLEVIKNGKEFFTK"
     gene            3180..4244
                     /locus_tag="KPD58_001963"
     CDS             3180..4244
                     /locus_tag="KPD58_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265729.1"
                     /translation="MEKNFLLNKINSIQKEYLELLVKLKDNIDTIDYIYIIDEINIFW
                     YSYRDVIKLYLGNITQKDNAYIFTGATYLDVDDLEHYPFVMMGDIHIVDDSLCKYSKT
                     VLVLSNQEFAKVLKDQIISCIDDNIKILSNYSFAMLILPMNLLYGNDMKIIHEYSNNI
                     FLNFFSRKDLNLDTYFEEFNSIEDIALALNPGIESYIVFDEGEDLTKNLEERFKSFID
                     KGKVPLPAHKSDAFKFWLIIYGFITQALDIILSCKGFNLTPYLRYRVTLQYVLLLGDN
                     VFDIEDLSNMLLKCKVAHVLYAIFDKSEIKNISFVQFYKKLSEYKFSYKVENDIKENI
                     NINQIAEILNKNITLFLNYI"
     gene            4384..5556
                     /locus_tag="KPD58_001964"
     CDS             4384..5556
                     /locus_tag="KPD58_001964"
                     /inference="COORDINATES: protein motif:HMM:NF013752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG0265730.1"
                     /translation="MLKQNTIQIDYTTYGNNYQFKLPLNIDYMIPKDAIFVDGTKIES
                     SANRYTFVWKKTINKNMVRVMNDISNFILKCGIDFGIKIIYRNKIKIYHLKKLLKKLK
                     KISKESNITFVHGKGKKKSIVQKSIAKLNNYIDRLKKYTKDLHIMGELNSYSKTDNDA
                     TFMRMKKDHMKNGQLKPAYNIQFGVDSEYIVWVSSEHKPTDTTTSIPFLKSIEEHTKF
                     KYPKIVADSGYESEENYTFLECNNQLSFIKPANYEISKTRKYKNDIGRIDNMDYDEKK
                     DYYICKNGKKLFANKIINRKSKTGYKSKITCYTCKECCDCQYKNGCIKGNNSKIPLEN
                     RTKNLQVSKLFHEKRKENLKRIISQEGCQLRMNRIIQAEGTFAQVKQNMKFRRFLS"
     gene            5998..6309
                     /locus_tag="KPD58_001965"
     CDS             5998..6309
                     /locus_tag="KPD58_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0265731.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMESAAKENGIESQIWAIPESTIQN
                     EIEKCDVLLLGPQVRYVLPKAQEIARPYNIPVEVINMMHYGTVNGEAVLNRAIELNNT
                     K"
     gene            6357..6674
                     /locus_tag="KPD58_001966"
     CDS             6357..6674
                     /locus_tag="KPD58_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG0265732.1"
                     /translation="MDEKIIEISFNIIGYAGEGKGLAFEAIKEAKNGNIEKAKELLKE
                     SREVINKAHRYQTELIQNEASGNRTEISVILIHAQDHLMNGMNFQQLAEEIVDLHLKL
                     QDK"
     gene            6887..7588
                     /locus_tag="KPD58_001967"
     CDS             6887..7588
                     /locus_tag="KPD58_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0265733.1"
                     /translation="MLRNQPLYIQVVDILKQKILNNEYKIDENIPTERELEQEFGVSK
                     ITIRKAIEILESEGYVEKKSGKGTKVISNSLFNKLSKAESFSSILLSKGHKLSKKTIS
                     IEKIINDTNSQVFSIFGEKCSKITRMYYLDSKPYIFFTHYIPFDENLTVESFNNESSL
                     YMYLYRRNINITRFEDEFFIEESELEVSKALNLAKDILLGRKRKTYDENDNIVELSFA
                     RYNTEVQNYVIKYEI"
     gene            7813..9114
                     /locus_tag="KPD58_001968"
     CDS             7813..9114
                     /locus_tag="KPD58_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0265734.1"
                     /translation="MEKFASFLERKIMPVAAKISNQRHMRAVRAGIIATLPLTIVGSF
                     FTILLNIPIDAYMDFIAPFKAILDIPFRYTVGFLSLYATFGIASSLAKSYDLDTTGVG
                     MLAVMAFLVSTIVPTQVVEPVGGVIEAGRWMDMAKLNSPSLFGAIVTSLITVEIYRFM
                     KEKNITIKMPAGVPVEVSNSFTALLPTAVILMLFWVIRHILGFDISVALSAMLSPLKG
                     FLSGNSLGGGLLTVFLITLFWVLGIHGPAIMGPVIRPIWDMSIAENITAFTEGVSAHA
                     MPNIFTEQFLQWYVWIGGAGATLALVVMFMFSKSQYLKSLGRLGFLPGLFNINEPIIF
                     GAPIVMNPILGIPFIIGPIIMTILSYVLTITGVIPMMVARLPFAMPAPIAAVMSTNWS
                     ILAGILVLINFVISFAVYYPFFKVFEKQQLQREQEEMSENL"
     gene            9251..10345
                     /locus_tag="KPD58_001969"
     CDS             9251..10345
                     /locus_tag="KPD58_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBG0265735.1"
                     /translation="MERRLGISVYPEHSNMEDDKAYIKMASKYGFKRIFMCMLSITRP
                     KEEVKKHFKEIITYARELGFEVILDIAPNIFDILQISYDDLTFFSELNASGIRLDYGY
                     DGSKEAMISFNPYDLKVELNMSNDVAYLDNIITYKPNVNNIYGCHNFYPQRGTGLPYD
                     FFVRCSNRFKKYGIRTAAFVNSKYATIGPWNINDGLCTLEMHRNLDIATATKHLFATG
                     LIDDVIVGNAYASEDELKRMSEVNEYQVVFNVVAEEKTSEVEKIIMVNEQHFRRGDIT
                     NQVARSTEVRKKYAKENFPKHDNKNLFKRGDVVIGNNDFGKYKGELQLILQDCTDERK
                     NKVGRIKEDEIILLDFIEPWSKFKIILEGE"
     gene            10347..11480
                     /locus_tag="KPD58_001970"
     CDS             10347..11480
                     /locus_tag="KPD58_001970"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HBG0265736.1"
                     /translation="MKIDILIKCGKTVDKSIIDIAILDDKIIEVKNQIDDSKYQAKQI
                     INLDGEKFISAGWIDIHTHCYERLDLYKDYPDEVGIKSGVTTVVDAGTTGALDIGKFY
                     EDTKKYKTNVYALINIAKQGITSQDELSSMMNIDEYELKRAVKKYKDFVVGIKARMSK
                     SVVISNDVEPLKVAKRIKNELNLPMMVHFGSSPPTIEDIFDYMEKGDILTHIYNGKPN
                     GILRGNEVKKEIIEARERGIILDVGHGTESFSMDIAMKSKDAGIFPDTISTDIYIKNR
                     INGPVYNLSTTMEKFIYMGYSLEDIINKVTKNAADAISLKNKGLIKEGYDADLTIFDV
                     VNEEKELQDSLNKSVITSTSIKPRAVVVNGEYLNIGKSIEENE"
     gene            11498..12607
                     /locus_tag="KPD58_001971"
     CDS             11498..12607
                     /locus_tag="KPD58_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HBG0265737.1"
                     /translation="MSSIFEKYSVRRVINASGKMTILGVSTVDKEVIEAIGEGCKNFF
                     IIEELINKTGEYIANLLGCESALIVSSASAGIAQSVGAMIAKDDMSLVYNVNNPKKQV
                     KREIIIPKGHNVDYGVPVEVMIRLGGGEVVEAGYANMCNKEHIIREINENTAAIMYIK
                     SHHCVQKSMLSVLDVVQVAKEYNLPLIVDAAAEEDLNVYYELGADLVIYSGAKAIEGP
                     SSGLVIGKKKYIDNVKLQSKGIGRAMKIGKENIVGLTCAIERYISNDKVTLKEMEDKL
                     NPFIDKINTIKGVSASITRDSAGREILRGEIDFNEDIINKSTQQIINELRSGEIAIYT
                     RDYKANEGKIEIDIRSVTGSDLDTIYNRIKNIVES"
     gene            12672..13412
                     /locus_tag="KPD58_001972"
     CDS             12672..13412
                     /locus_tag="KPD58_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HBG0265738.1"
                     /translation="MNCIPQYYKNRVCLNVLAGSIKNAKEVYDACDGHVLIGVLSKNY
                     STVEAAIEDMKKYSKEIDNAISVGLGAGDPNQSNMVSQISKELQPQHINQVFTGVATS
                     RALLGQNDSIINGLVSPTGKPGFVKISTGPLSSKEKDGIVPIETAIAMMKDMGGSSIK
                     FFPMGGLKTKDEYIEVAKACAKHNFYLEPTGGIDLDNFKEIVQIALDAGVEKVIPHVY
                     TSIIDKETGETKVEDIGVLYKIMKELLG"
     gene            13417..14409
                     /locus_tag="KPD58_001973"
     CDS             13417..14409
                     /locus_tag="KPD58_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBG0265739.1"
                     /translation="MNILAFGEIMMRLSVPDYKFLTQTNELNYIITGTGLNILSGLKN
                     FGYNTYMLTKLPNNNVGKALSANIRKLGVKDDFITYGGNHIGVYFLENGYGERPSEVT
                     YLNRLNSSFCESKLQDYDIDKCLEGMDIVHICGIAMQLTDSVKEIALELARKSYEKGI
                     KVFFDFNFRQSLNPERTYDDLVNDYKKILPYCHGLFASYRDINKILNIDSLNIKSSSE
                     LIKKEYDIGIVAGTMRRFDDMNNRFIKGYISNDDGYFESREYKLEIYDRVGAGDGYVA
                     GIIYSYLENMKSEDIVEFGVASSVLAHTTYGDNSLVSTGHVLRLMNNEKIDILR"
BASE COUNT         5948 a         1403 c         2586 g         4561 t
ORIGIN      
        1 gcaaaatcct atccaagagt attatgagtt tcttgaagat atttggaaaa ataacataaa
       61 aatagatgaa ttaattaata agtatttaat tacagcaaca agaatggatc aaattaaatt
      121 cccattaata tatattgatg aagattcttt ttgtccttat tgtttaggta ataaattcaa
      181 tattattaat aatattaagc aacaagatat aaaagttgaa tgtgaaaagt gtggtgaggc
      241 aattagttat tttattcata aggaagaagt agataatata atagaagaag aaaaaagaaa
      301 aaaagaacta tttataagaa cattggagaa tgtatataat aaaataaaca agattaaatg
      361 tcatagatgc caatcagatt tatatgtgtg tattaatgat aaaataatgg agtataatat
      421 taggtgtgat agatgtaatt cacagtatga atcggttgaa attgcacagg aacaatatag
      481 attatggcaa caaagagctg caatgatggc taacataaaa aataaagaaa atgaattgtt
      541 gctaaagaca ctatcaatta aggacgaaaa agatattaaa tttaaactcg aagatataat
      601 tacggagagt gaaagtatag atgtaattag tgatttagta gatatgcaag atagaggaga
      661 gaatattttc aagcaattat ttaataatta tgttagacta ggaagattag acagaaggtt
      721 attggtaact gtgtgtaaag attgcaagca aaatgaaaat acttatacat gggaaagagg
      781 caaagaacat ttaataaata tgagggggat tacatacagt gaagcagttg tatatgagtt
      841 aatggaaaaa acaaagctat tgccaataag agcaattatt agaagactta tggagaaaaa
      901 actattgtat tgtgatgaaa ggagcaataa tttacaagta caccctgttt tgattgaaaa
      961 tttagctaaa ctagaatcaa taaataaatc tcaaaatata aataatcaag tagcttatct
     1021 aatattcaat aagcaaaaat atatatgtta tcattgtggt gaatcaggaa gacccttaaa
     1081 gataggatat ctaacaatag ataaaaatat aaatgattta tcaagtttag tgggaatatg
     1141 tgatagttgc tatgaagata ttactgaaga tgaaattatt atagataata ttttttgtgt
     1201 ggtagatgaa agaaacaatg aattagggga agcttggaaa tttttaatac aatatgatag
     1261 tagttttaga aataatacaa ttgcagaagt agagcatgca agattatatg atatgtatga
     1321 tgaagataat ttaattcgtg cgtatgcaaa tacattgtat aaacaaacga aagatgggtt
     1381 tgctaatgaa aaagtatttt ttgcatatgc aagagcaata ttgaaaaata gcaaagggca
     1441 agtaagtgta ctacaaatag caaaagaaaa atttaaatta gaaaagtggt taaatggata
     1501 aagggataat acatattaaa attataaatt tatataaaac tgtattgcaa ttaagcaata
     1561 cggtttttat atataagata atgatacaat taaccaaaat gtggtagtac ataagtgtta
     1621 tgagatttaa atattagcga ttaaaataat gattaagtaa aattgtaatt gatgaggagt
     1681 attaacgtat tagtttattt ttatatataa ttaaaatata ggagggaaga atgtgagtca
     1741 tagtaaacta tctgatcaca aattaaaaaa ggggaaattt atagcacctt ttaatgaatt
     1801 tatgacacca ttgaatagta atgagtcatg gtgccttggt cgattgccag agtatatatg
     1861 gataggatta ttaataaata aatatggtag aaaaaaaggg atggaaataa tttataaaat
     1921 aattgtaaaa cttcattcaa ttgaatcaga tattgtaaca cctaggttgt ctgagatttt
     1981 atctatggag gatgtaaagc aggatgaatt ctttagttat attctaacta ttgttgaaaa
     2041 ggaagcttta tctccattga cattaatatt tacatatagt aattatccta tttttgttaa
     2101 atattttgca gagccaagta taaaaattga agatagggtt acctatttag atgagacttt
     2161 aagaaaagct atgcatcatc aaagtgaatt tgctacagac ataagattta tagttttata
     2221 ttatttttgt atgtcaggaa aactcgtatt gcaaaaagaa cagattaata atttgctgga
     2281 atatcctaat ttagaacaca ctgatgaaaa aatgagaatg ttacgtccgt ttattcgtgc
     2341 agcagaatta atggttttaa atatggaaaa aaaatctgat tggtatttag atattttttg
     2401 ggggaggatt agtgttatga ctgaatgtaa attatacgca actaaatttg aagatgaaac
     2461 tgctaataca gacttgtata tggaacaatt acatgagatt tttgtttatt tatcagattt
     2521 actatgtaaa gtaaatccat tagacgagaa aatgttagta ttacttggaa tagcaatata
     2581 ttcttataaa cgattacgag aagtagttga gcataaatta tttaattcca tttcaggtag
     2641 aggctcagta agggtattga tagaaaatta tattatgatg aagtatttag taaaaataga
     2701 agctcaacat gaaaatatat ggtcagaata tcaattttat ggaataggtc aatataaatt
     2761 aattgtggct agatatagag aacaagaaca agatttaaaa aatagtcatg ttgattataa
     2821 atatattgaa gctttagtta atgaatttgt aattgaagaa acaatagata tggatactaa
     2881 atattttgat aagcaaaata tacgtagtaa ggctgaaata gttggagaaa aagagttata
     2941 tgggttgtac tatgattatg attccgcata tgaacatggt ttatggggat caataagaga
     3001 aagttcaatg ttaaagtgtg ataatccagc tcatcactat cattgtatac cagatgtaga
     3061 caataatcaa aatttaaaaa gtgtttggca tgattgtgta aacataatgg aaaaaacttt
     3121 acttttctta gatactgtat atggtatacc acaacatttg ctattagagg tgataaaaaa
     3181 tggaaaagaa ttttttacta aataaaataa atagtattca gaaggaatat ttagaattat
     3241 tagtaaagtt aaaagataat atagatacta tagattatat ttatataata gatgaaataa
     3301 atatattttg gtattcatat agagatgtta taaagcttta cttagggaat attacacaaa
     3361 aagacaatgc atatattttt acaggtgcaa catatctaga tgttgatgat ttagaacatt
     3421 atccatttgt tatgatggga gatatacata tagtagatga ttcgctatgt aaatattcaa
     3481 aaacagtact agtattatca aatcaggaat ttgctaaagt attaaaagac caaattattt
     3541 cttgtataga tgataatatc aaaatattgt ctaattattc ttttgcgatg ttaatattac
     3601 caatgaattt actatatggt aatgacatga aaataataca tgaatattca aataatatat
     3661 ttctgaactt tttttcaaga aaagacttaa acttagatac ttactttgaa gaatttaact
     3721 caatcgaaga tatagctcta gcattgaatc caggtataga gagttatatt gtttttgacg
     3781 aaggtgaaga tttaactaaa aatctggaag aaagatttaa aagctttatt gataagggga
     3841 aggtacccct accagcacat aaaagtgatg cttttaaatt ttggctgata atttatggat
     3901 ttataactca ggcattagat attatattgt catgtaaagg atttaaccta actccttatt
     3961 taagatatag agttacatta caatatgttt tattactagg cgataatgtt tttgatattg
     4021 aagatctcag caatatgttg ttaaaatgta aagtagctca tgtactttat gcaatatttg
     4081 ataaaagcga aataaaaaat atttcctttg ttcaatttta taagaaatta agtgaatata
     4141 aatttagcta taaagtagaa aatgatatca aagaaaatat aaatataaat caaattgctg
     4201 aaattttaaa taaaaatatt acattgtttt taaattatat ataaatttat ttatataatt
     4261 acattcctta gggtacataa cagaaaaaag gctgtcgcac taaaaaatag tgctacgccc
     4321 ttttttatac aaaaaaataa agctcaagtt caaaagaacc tgggattcct gtataataat
     4381 tgtatgctaa aacaaaatac tatacaaatt gattatacta cttatggtaa taattatcaa
     4441 tttaaattac cactaaatat tgattatatg attccaaaag atgctatatt tgttgatggt
     4501 acaaaaatag agtcatctgc caatagatat acttttgttt ggaaaaaaac tataaacaaa
     4561 aatatggtta gagtgatgaa tgatatatct aatttcatat taaaatgtgg gattgatttt
     4621 ggtattaaaa taatttatag aaataaaatc aaaatatatc atttaaaaaa acttttaaag
     4681 aagcttaaaa aaatatcaaa agaatcaaat ataacatttg ttcatggaaa aggaaagaaa
     4741 aaatctattg ttcaaaaatc tatagcgaaa ttaaataatt atatcgatag attgaaaaaa
     4801 tatactaaag accttcatat aatgggtgaa ctaaatagtt attctaaaac agataatgac
     4861 gcaactttca tgagaatgaa gaaagaccat atgaaaaatg ggcaattgaa accagcatat
     4921 aatatacagt ttggcgtaga ttcagaatac atagtttggg taagttcaga gcataaacct
     4981 actgatacta caacatcaat accattttta aaatctattg aagagcatac aaaatttaaa
     5041 tatcctaaaa tagtagctga ttctgggtat gaaagtgagg aaaactatac atttttagag
     5101 tgtaataatc aattatcttt tataaagcct gcaaattatg aaattagtaa aactagaaaa
     5161 tataaaaatg atattgggcg tattgataat atggattatg atgaaaaaaa agattattat
     5221 atttgtaaaa atggcaaaaa actatttgct aataaaataa tcaatagaaa atcaaaaaca
     5281 ggatataaaa gtaaaataac ttgttatact tgtaaagaat gctgtgattg tcaatataaa
     5341 aacggttgta ttaagggaaa taatagtaag attcctcttg aaaatagaac taaaaattta
     5401 caagtatcta agttatttca tgaaaaaaga aaagaaaatt taaagagaat tataagccaa
     5461 gaaggttgtc aacttagaat gaatagaatt atacaagcag aaggaacttt cgctcaagta
     5521 aaacaaaata tgaaatttag aagattttta agctgaggca aagaaaatgt tatatcagaa
     5581 tgtattattg tagcgatagc tcataacatt tctagactac acaacaaaat aataaaaaat
     5641 aaatgtggaa tatatcttca tcatttaaaa gaagtattct aaatttatta tttatagtta
     5701 gatattatct gaaaaaatgg tatttttata cctttatttg ttatgcataa ttttacttag
     5761 aacataagct ttatataaaa caattaaaaa tatatctaat taagataagt aaaaaaagct
     5821 atcacagaac aaatttaatt tgctaatgcg acagcccatt tttgttaatt tgacacttaa
     5881 taatattaaa ataacaaaaa agtattgaaa gtatattgca tttaaggtag aattaaataa
     5941 aatattataa cattataatg tttatatagt tgttaataaa aatttggagg aaaatatatg
     6001 ataaaaataa tgttggcctg ttcagcaggg atgtctacaa gtttattagt tactaaaatg
     6061 gaaagtgcag ctaaagaaaa tggtatagag tcgcaaattt gggcaatacc agaatcaact
     6121 atacaaaatg aaatagaaaa atgtgatgta ttattactag gaccacaagt tagatatgta
     6181 ttacctaagg ctcaagaaat tgctaggcca tataatatac cagtagaagt tataaatatg
     6241 atgcactatg gaacagttaa tggagaagct gttttaaata gagcgataga acttaacaac
     6301 actaagtaat ataaattata atgtattttt aatatataaa ttgggagaat caaaggatgg
     6361 atgaaaagat tatagaaata tcttttaata taataggtta tgcaggtgaa ggtaaaggat
     6421 tagcttttga agcaataaaa gaggctaaga atggaaatat agaaaaagca aaagaattat
     6481 taaaagagtc tagggaagtt ataaataaag cacatagata tcaaacagaa ttaatacaaa
     6541 acgaagctag tggaaacagg actgaaataa gtgtcatact tatacatgca caggaccact
     6601 taatgaatgg catgaatttt caacagttag ctgaggaaat cgttgattta catttgaaat
     6661 tacaagataa ataaaaatta aagataataa attaagagag ttcttaaaag atacattatg
     6721 tgtagcacac taattgttag attattgatt caatatttag tgagtaatac attaggatta
     6781 ttttgaggac tctttttata tttagtttaa agttaataaa tatttattat taataatata
     6841 agattatatg ttataatatt ataacatata atttaagagg tgaatcatgt taagaaatca
     6901 accattatat atacaagttg tagatatatt aaagcaaaaa atattaaata acgaatataa
     6961 aattgatgaa aatattccaa cagaaaggga gttagagcaa gagtttggag tcagtaaaat
     7021 tactattaga aaagctattg agatattaga atcagaaggg tatgtagaga aaaaaagtgg
     7081 aaaagggaca aaagtaataa gtaatagtct ttttaataag ctatcaaaag cagagtcttt
     7141 ttcaagtata cttttatcta aaggtcataa gttatctaaa aagactattt caattgaaaa
     7201 aataataaat gatacaaatt cacaagtatt tagtatattt ggagaaaaat gtagcaagat
     7261 tactagaatg tattacttag atagtaagcc atatatattt tttactcatt atattccttt
     7321 tgatgaaaat ttaactgttg aaagttttaa taatgaaagt tctttatata tgtacttata
     7381 taggagaaat ataaatataa ctagatttga agatgaattt tttatagaag aatcagaact
     7441 agaggtatca aaggctctaa atctagctaa agacatatta ttaggaagaa aaagaaagac
     7501 ctatgatgag aatgataata tagttgagct atcatttgca agatataata cagaagtaca
     7561 aaactatgta attaaatacg aaatataata agaatcctga gtttattgaa atgataatat
     7621 gctcaggatt tttgtttagt tgtaaaaaca aaaaaatatt ataacattat aatgtttaca
     7681 accaaacatt ataatgttat aatattttta ataaataagt gatattatca attacataaa
     7741 aattagttta tgttatgaaa tcgttattgc attataaata aatgataata tcaataatac
     7801 agggggataa ctatggaaaa attcgcaagt tttttagagc gcaagattat gcctgtagca
     7861 gcgaagataa gtaatcagag acatatgaga gctgtaagag caggtataat agcaacatta
     7921 ccactaacaa tagtggggtc atttttcaca atattattaa acatacctat tgatgcttat
     7981 atggatttta tagctccatt taaagcaata ttagatatac catttagata tactgtagga
     8041 tttttatctc tatatgctac ttttgggata gcatcatcac ttgctaaaag ctacgattta
     8101 gatacaacag gtgtcggtat gcttgcagtt atggcatttt tagtttctac aatagttcca
     8161 actcaagtag ttgaaccagt aggtggagtt atagaagctg gtagatggat ggatatggca
     8221 aaacttaatt cgccatcatt atttggagct atcgtaacat cgttaattac agttgaaata
     8281 tatcgtttta tgaaggaaaa gaatattaca attaagatgc cagcaggagt tccagtagag
     8341 gtttcaaatt catttactgc attattaccg acagcagtaa ttttaatgtt attctgggta
     8401 ataagacata ttttaggatt tgatataagt gttgcactaa gcgctatgtt atcaccttta
     8461 aaaggattct tatcaggaaa tagtttaggt ggtgggttac tgacagtatt cctaataact
     8521 ttattctggg tacttggaat acatggtcca gctataatgg ggccagtaat aagacctata
     8581 tgggacatgt ctatagcaga aaatataact gcattcacag aaggagtatc agctcatgca
     8641 atgccaaaca tatttacaga acaattttta cagtggtatg tatggatagg gggagcaggg
     8701 gctactcttg cattagttgt aatgtttatg ttttctaagt cacaatatct aaaaagttta
     8761 ggtagattag gatttttacc agggttattc aacataaatg aacctataat atttggagca
     8821 ccaatagtta tgaatccaat actagggata ccatttataa ttggacctat aataatgaca
     8881 attctatcat atgttcttac tattacaggg gttataccta tgatggtagc aagattacca
     8941 tttgcaatgc cagcaccaat agcagcagta atgagtacta actggagtat actagcaggt
     9001 atcttagtat taatcaactt tgtgatatca tttgctgttt attatccatt cttcaaagtt
     9061 tttgaaaaac aacaattaca aagagaacaa gaagaaatga gtgaaaattt ataaaaatac
     9121 atagatatag aggttttgta ctatataaaa tatagaaaaa gcttataagt tattaagggt
     9181 gaaggtagga ggaatgacct ttgcctttaa ttttatatat tttaaataat gtaatttgag
     9241 gaggagaaag atggaaagaa gattaggaat atcagtatat ccagagcata gtaacatgga
     9301 agatgataag gcatatataa aaatggcttc aaaatacgga tttaaaagaa tatttatgtg
     9361 catgctttca attacaagac ctaaggaaga agttaaaaaa cattttaaag aaattataac
     9421 ctatgcaaga gaattaggat ttgaagtaat ccttgatata gctccaaaca tatttgatat
     9481 attacaaata tcttatgatg atttaacatt ttttagtgag ttaaatgcta gtggaataag
     9541 gcttgactat ggatatgatg gaagtaaaga agctatgatt tctttcaatc catatgattt
     9601 aaaagtggaa ctaaatatga gtaatgatgt agcatacttg gataatataa ttacttacaa
     9661 gccaaatgta aataatatat atggttgtca caatttttat cctcaaaggg gtacaggctt
     9721 gccatatgat ttttttgtaa gatgttcaaa tagatttaaa aagtatggaa taagaactgc
     9781 tgcttttgtt aattcaaaat acgcaacaat agggccttgg aatataaatg atgggctgtg
     9841 tacgcttgaa atgcatagaa acttagatat tgctacagca actaagcact tattcgcaac
     9901 aggattgatt gatgatgtaa ttgtagggaa tgcatatgca agtgaagatg aattaaaaag
     9961 aatgtctgaa gtaaatgaat accaagttgt atttaatgta gtagctgaag aaaaaacttc
    10021 tgaagtagaa aagataataa tggtaaatga gcaacacttt agaagaggag atataactaa
    10081 tcaggtagca agatctacag aagtaagaaa aaaatatgct aaagaaaatt tccctaaaca
    10141 tgataataag aacctcttta aaagaggaga tgtagtgatt ggtaataatg actttggaaa
    10201 gtataaaggg gaattacagt tgatacttca agattgtaca gatgaaagaa aaaataaggt
    10261 gggtagaata aaggaagacg aaataatact tttagacttt attgaacctt ggagtaaatt
    10321 taagattatt ttagagggag agtaaagtga agatagacat attaataaaa tgtggaaaaa
    10381 cagtagataa aagtataatt gatatagcta tattagacga taaaataata gaagtaaaaa
    10441 atcagataga tgattctaag tatcaggcaa aacaaattat aaacttagat ggagagaaat
    10501 ttattagtgc tggttggata gatatacata cgcattgcta cgaaagacta gacctttata
    10561 aggattatcc agatgaggtt ggaataaaaa gtggagttac tactgtagta gatgcaggaa
    10621 caacaggagc tttagatata ggtaaatttt atgaagatac taaaaaatat aaaacaaatg
    10681 tatatgcttt aataaatatt gctaagcagg gaataacatc tcaagatgaa ctttcaagta
    10741 tgatgaatat tgatgaatat gaacttaaaa gagcagttaa aaaatacaaa gattttgttg
    10801 ttggaatcaa agctagaatg agtaaaagtg ttgtaatttc aaatgatgtt gaacctttaa
    10861 aagtagctaa aagaataaaa aatgaactta atctacctat gatggttcac tttgggagtt
    10921 ctcctccaac gatagaggat atatttgact atatggaaaa aggagatata ttaacccata
    10981 tatataatgg aaagccaaat ggaatactta gaggcaatga ggttaaaaaa gaaatcatag
    11041 aagctagaga aagaggtata atccttgatg taggtcatgg aaccgaaagt tttagtatgg
    11101 atattgcaat gaaatctaag gatgcaggaa tttttccaga cactataagt acagatatat
    11161 atattaaaaa tagaataaat ggtccagtgt ataatctatc tacaacaatg gaaaagttta
    11221 tatatatggg ctattcactt gaagatataa taaataaagt tactaaaaat gcagcagatg
    11281 caataagttt aaaaaataaa ggtttaataa aagaaggata tgatgctgat ttaactatat
    11341 ttgatgtggt taatgaagaa aaagaacttc aagattcttt gaataagtca gtaatcacaa
    11401 gtacgtctat aaagccaaga gcagtagtag tcaatggaga atatttaaat ataggtaagt
    11461 ctatagaaga gaatgagtag aagggagaaa ctattgtatg agtagtatat ttgaaaaata
    11521 ctctgtaaga cgtgttataa atgcttcagg caaaatgact atactaggag tatcaacagt
    11581 agataaggaa gttattgaag caataggtga agggtgtaaa aatttcttta taatagaaga
    11641 acttataaat aaaacaggtg aatatatagc aaatttatta gggtgtgaaa gtgcactaat
    11701 agtttctagt gcatctgctg ggattgcaca gagtgtggga gcaatgatag caaaagatga
    11761 tatgagtcta gtttacaatg taaataatcc taagaaacaa gttaaaaggg aaataataat
    11821 tccaaaggga cataatgttg attatggagt tccagtagaa gtaatgataa gacttggagg
    11881 aggagaagtt gttgaggccg gttatgctaa tatgtgtaac aaggaacata taataagaga
    11941 gataaatgaa aatacagcag caattatgta tattaaatct catcattgtg ttcaaaagag
    12001 tatgctaagt gtattagatg tagtacaagt tgctaaagaa tataatttac ctttgatagt
    12061 agatgcagca gcagaagaag atttaaatgt atattatgaa ttgggagcag acctagtgat
    12121 atacagtggt gctaaggcta tagagggtcc tagctcaggg ttagtaatag gtaagaaaaa
    12181 atacatagat aatgtaaagc ttcaatcaaa gggtatagga agagctatga aaataggaaa
    12241 agaaaatatt gtaggtctaa catgtgcaat tgagagatat ataagtaatg ataaagtaac
    12301 tttaaaagaa atggaggaca agctaaatcc atttatagac aagataaata ctataaaggg
    12361 agtgagtgct agtataacta gggattcagc aggtagagaa atacttcgtg gagaaattga
    12421 ctttaatgaa gatataataa ataagtcaac acaacaaatt ataaatgaac ttagaagtgg
    12481 agagatagct atatacacta gagactataa ggctaatgaa ggcaagattg agattgatat
    12541 aagaagtgta actggaagtg atttagatac tatttataat aggataaaaa atatagtaga
    12601 aagttagagt gtagagtaag tgacattata atgatttaat aaattaaatt ttaccaatgg
    12661 aggggataaa tatgaattgt ataccacaat attataaaaa tagagtttgt ttaaatgttc
    12721 ttgcaggaag cataaaaaat gctaaagaag tatatgatgc ttgtgacggg catgtattga
    12781 taggagtact atcaaagaat tacagtactg tagaagcagc tattgaagat atgaaaaaat
    12841 actcaaagga aatagataat gcaatatctg tggggcttgg agctggagac ccaaatcagt
    12901 caaatatggt atctcagatt tctaaagaac ttcagccaca acatataaat caagtgttta
    12961 caggtgtggc tacgtctaga gcattacttg gtcaaaatga tagtataata aatggattag
    13021 tttctccaac tggtaaacca ggatttgtaa aaatatccac aggtccatta agttcaaaag
    13081 aaaaagatgg tatagttcct atagaaacag caatagctat gatgaaagat atgggaggaa
    13141 gctcaattaa gttttttcca atgggaggac ttaaaacaaa ggatgaatat atagaagttg
    13201 caaaagcttg tgcaaagcat aatttttatt tagaaccaac aggaggtata gacttagata
    13261 acttcaaaga gatagttcaa attgcacttg atgctggtgt agaaaaagtt atacctcatg
    13321 tttatacatc aataattgat aaagaaacag gtgaaacgaa agtggaagat ataggagtac
    13381 tttataaaat aatgaaagaa ttattgggat agatttatga atatattagc ttttggtgaa
    13441 ataatgatga gactttcagt acctgactac aagttcttaa cacaaaccaa tgaactaaat
    13501 tatataatta caggcacggg attaaatata ttatctggac ttaaaaactt tggatacaat
    13561 acctatatgt tgacaaagtt gcctaataat aatgtaggta aagcattaag tgcaaatata
    13621 agaaagcttg gagtaaaaga tgactttatc acatatggag ggaatcatat aggagtgtat
    13681 tttcttgaaa atgggtatgg agaaagacct tctgaagtga cttatttgaa taggttaaat
    13741 agttcatttt gtgagagtaa attacaagat tatgatattg ataaatgttt agaaggaatg
    13801 gatatagtcc atatttgtgg tattgctatg caacttacag attcagttaa agagattgct
    13861 ttagaactag ctagaaaatc ttatgaaaag gggataaaag tattctttga ttttaatttt
    13921 agacaatcat taaatccaga aaggacttat gatgatttag taaatgatta taagaaaata
    13981 ctaccatatt gtcatgggct gtttgctagt tatagagata taaataagat acttaatata
    14041 gatagtctaa atataaaatc tagttcagaa ttgattaaaa aagagtatga tataggtatt
    14101 gtagctggaa ctatgagaag atttgatgat atgaataata gatttataaa gggatatata
    14161 tcaaatgatg atggttattt tgaaagtagg gagtataagt tagaaatata tgacagggtt
    14221 ggtgccggag atggatatgt agcaggaatt atatattcat atttggaaaa tatgaagagt
    14281 gaagatatag ttgagtttgg ggttgctagt tcggtgttag ctcatacaac ttatggggat
    14341 aattctttag taagcacagg tcatgtactt agactgatga acaatgaaaa aatagatata
    14401 ttgagataat acatttgtat ataataaaat ttttatcttt tttagacaca tttttgactt
    14461 gcttatattt gttaaacaga gttattttta atgattat
//
LOCUS       DAELLV010000084        14477 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.084, whole genome shotgun sequence.
ACCESSION   DAELLV010000084 DAELLV010000000
VERSION     DAELLV010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14477
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.084"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            673..2343
                     /locus_tag="KPD58_001974"
     CDS             673..2343
                     /locus_tag="KPD58_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF and EAL domain-containing protein"
                     /protein_id="HBG0265740.1"
                     /translation="MREESNSLSLDHIEIFFELLSQSTEDYIFFWDINRNKFKISSAI
                     FDEFNLSKEIESDLVNCWSKIVYPDDVQIWKDDIQELLHGKKGEHNLEYRLINKYKEI
                     VWISCRGKVYVSDDPKTIFLVGRIKNIGEKNKFDSITGTWNREQFEHRMNYLIKEKIY
                     KNGAMFIMDIDNFKNINEKYGHSYGDKVLRAIATEVLEYLPKDVRLYRLDGDEFAFFY
                     PMCTKETIEKIYEKIQMYTNTQHEIESNKYYCTVTAGVAMYPEDGDNYLDLFKHADIA
                     LDIAKISGKNRIKFFSQELYENKLKVISMQQKLRECVENNFNDFELFFQPQVNAVTKE
                     VIGAEVLLRWHSSTYGEVSPVEFIPILEQSNLIIPVGKWIIKEAVKQCKEWHKINPDF
                     KISVNVSYIQLKEDFFRDFIVECLVEYQLRPEFLILELTENCWIPDINLLNDKFISLK
                     GIGVYIAIDDFGTGYSSLNYLKELSVNIIKIERSFVKNITYNSYEYTFLEYIIKLAHI
                     INLKVCVEGIESYEEYDIVKSLGVDIIQGFLFGRPVSASEFYKFQLSK"
     gene            complement(2465..3199)
                     /gene="pflA"
                     /locus_tag="KPD58_001975"
     CDS             complement(2465..3199)
                     /gene="pflA"
                     /locus_tag="KPD58_001975"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HBG0265741.1"
                     /translation="MIKGKIHSIETFGTVDGPGIRYILFFQGCPLRCKYCHNRDTWDI
                     KSGKEYTVDEIITDALKYTSFMKFSGGGITASGGESTLQPEFLSELFKKAKENDIHTC
                     LDTSGFVDIETIDPVLDNTDLVLLDLKHMVEEKSIDLTGVGMDKALKLARHLESRNIP
                     VWIRHVLVPGITDDIDNLEKLGQFVATLKNVERFELLPYHSMGIHKWESLGIDYELKD
                     VPDATKEDVQKASEIISKFGVKVYNS"
     gene            complement(3359..5590)
                     /gene="pflB"
                     /locus_tag="KPD58_001976"
     CDS             complement(3359..5590)
                     /gene="pflB"
                     /locus_tag="KPD58_001976"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBG0265742.1"
                     /translation="MNAWQGFKTGRWTKEINVREFIQLNYSPYEGNDSFLAGATENTK
                     KLWDEAMVLFKKERENGGTLDVDTKTVSGIAAYAPGYLDKELETIVGLQTDAPLKRAV
                     MPYGGIKMVENSCEAFGYELDPEIKDIFTKYRKTHNQGVFDVYTPEMRAARKSGIITG
                     LPDAYGRGRIIGDYRRVALYGVDALIEDKNEQKKSLEVSCMDEEVIRLREEITEQISA
                     LNELKKMAESYGFDISKPATNSKEAVQWLYFGYLGAVKDQNGAAMSLGRTSTFLDIYF
                     ERDLKAGIVTEEELQEYMDHFVMKLRMVKFLRTPDYNNLFSGDPTWVTECIGGMGIDG
                     RTLVTKNSFRMLNTLYTLGPSPEPNLTVLWSTKLPQGFKDFCSKVSIDTSSVQYENDD
                     LMRAYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKTLLYAINGGVDEKSGVQVGPRF
                     EPITSEYLDYDEVMSKFEPFTDWLATLYVNTLNVIHYMHDKYSYEALEMALHDRDIFR
                     TMACGMAGLSVCADSLSAIKHAKVKTIRNEQGIAVDFEIEGDYPKYGNNDDRVDSIAV
                     ELVESFMNKIRKNKTYRNSYPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPM
                     HGRDTNGALASLSSVAKLPYEHAQDGISNTFSIVPAALGKDMTERINNLSAMMDGYFA
                     QNAHHLNVNVFDRATLEDAMEHPEEYPQLTIRVSGYAVNFIKLTKEQQLDVINRTFHG
                     KMA"
     gene            5883..6944
                     /locus_tag="KPD58_001977"
     CDS             5883..6944
                     /locus_tag="KPD58_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HBG0265743.1"
                     /translation="MEKRKSIHLHKLNIYLIGGGDRLFITVVLFLVGFLLITKGADIF
                     INCTVEIGKKTNISEIILGATIVSFATTLPEFTVSLLASIDGHTTMSLGNAVGSIICN
                     TGLALGLVVFISPFNVDKKMFFSKSLLLIVSVIVLILLSLDGVITRGDSLLLIIILIF
                     YMVNNYRSVVGKSDTKNRNIKNNTIKDISTKKGKNYRGFSVLEIVKILLLFATGLIMM
                     IIGSQILIESGVIIASFLNIPQGIVSLTIIALGTSLPEIVSSITAIRKNHHEISVGNI
                     LGANILNIVSVIAVSAIPNNIPILSQNRQLDIPFMILLLLIVIIPTLKSNKLSRIQGI
                     LMLFTYFLYISILYFMYII"
     gene            7035..8648
                     /locus_tag="KPD58_001978"
     CDS             7035..8648
                     /locus_tag="KPD58_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG0265744.1"
                     /translation="MNITKFEDLPISEGIKKAIAEMGFEEPSPIQAQSIPAILSGKDV
                     IGQAQTGTGKTAAFSIPILETIDPNNRSLQAVVLCPTRELAIQVSTEIRKLAKYSHGI
                     KTLPIYGGQPIDRQIKSLKSGVQVVIGTPGRTIDHINRKTLKMDNVKMIILDEADEML
                     DMGFREDIEMILSKIPEERQTTFFSATMPRGILELTKRYQKDPEHIKVVRKELTVSNT
                     KQYYIETRSSNKLEVLCRLVDVYDPKLSVVFCNTKRKADELVGDLQARGYFADALHGD
                     LKQTQRDIVMDKFRNGTIDILVATDVAARGIDVDDVECVFNYDLPQDEEYYVHRIGRT
                     GRAGREGMSFTFVFGKEMRKMKDIERYTKSKLIKHNIPTITDVEEKKVGTFFAQVKQT
                     IEEGHLTKQLQWLEGFCNDEDYAMVDIAAALVKLSLGEEMKEEIIEEKPRRERGDRKG
                     GTGAKDGMIRLFINIGRNQRVQAKDIVGAIAGEVGIPGKVVGTIDIYDKYTFVEIPKK
                     DAKTVIEKMKDIKIKGNKINIEKANKKKK"
     gene            8923..10908
                     /locus_tag="KPD58_001979"
     CDS             8923..10908
                     /locus_tag="KPD58_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBG0265745.1"
                     /translation="MSMTYMDPRVFLILEMVLEEDTNTLEQFSNVLGVSTRTIRNYIN
                     QINRELKGGIAKIVKNEKGTYSIRIEDESKLIEIVNFNRGKLSNFINLNSPDERINYV
                     LDILTMTNNPITIDDLAEEICIGRTTLIKDLKKADRILAEYDLELKRKPNIGMILVGE
                     EMDIRLLILDRLYENYIDVLENVNQINLVKDYDIECLRDELKKLFKKEELYITEQVLR
                     DVERYIIMSVLRNLNGYKFEKIDKRFEVIRCSDEYTLGLKLKALLEKIFNIVLNEKET
                     IFLTMPLIGRKAPSTKLALSSIKINDSVKEVVDEVTSFLLETSGIDFKEDKKLIENLE
                     YHLYFALNRMRFNIRVKNPLLQEIKERYTFPYSVAKIAAMIIEEKFDLKICDHEIGYI
                     ALHFGSYFEKNSRKVLNVNRVAVVCGTGLGTAQLIRIKIGKILGESVEVNTFSDIEAK
                     ANLDLLKYYDLVFTTVDFKTDLDVPIFRVNALFNEESIKKEIENAILLKNSINLKSIK
                     KIEKNIPFIGKLIEEDKFFILNKSTFMENLEDMLEKLVHKGIIDIEFKKKVLKREKKS
                     STALDNYIALPHSVNTNGENLYLAFGILEKPVIWDTKEIRIIILMIIPDKNVDSTDLI
                     IKSYEEVLEVGRDKKMVEELAKVKSFDEFYKIITKRR"
     gene            10913..11347
                     /locus_tag="KPD58_001980"
     CDS             10913..11347
                     /locus_tag="KPD58_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HBG0265746.1"
                     /translation="MDPVIFIIASLITVYLLNIFLGYLQLKDFNKNYIELKRKGRVAI
                     GRKKGRISSGTIVLILINDDGVIVETRKMQGVTVLARVKSFEGLVGKSLEYIEKSDLT
                     VYNKLLKKAILDAVKQYITFEKNKCEDTNNLSTDEECRMEVV"
     gene            11376..11936
                     /gene="srlA"
                     /locus_tag="KPD58_001981"
     CDS             11376..11936
                     /gene="srlA"
                     /locus_tag="KPD58_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418408.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBG0265747.1"
                     /translation="MTQFFQVLASGADSFMNLFRAGGQQFVGFVTDIVPLLVALLVTM
                     NAIINFIGTDRVERLAKKCSANIFTRYLILPVLGTFVFANPMTLSLGRFLPEKFKPSY
                     FAAASFSCHTMNGLFPHINPGELFIYLGIANGITTLGFSTADLAVRYLLVGIVANFIK
                     GVVTDYTTKFVEKQQGIALNSDIKIG"
     gene            12000..12368
                     /locus_tag="KPD58_001982"
     CDS             12000..12368
                     /locus_tag="KPD58_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIB"
                     /protein_id="HBG0265748.1"
                     /translation="MAFNKVKIVKGSGGWGGPLIIEPTEKKNKVVYVTGGAKPETAVK
                     IAELTGCELIDGFSQGVKDDEIACVIINCGGTLRCGIYPQKKIPTINIMKTGKSGPLA
                     MFIKEDIYVSGVKPTNVEQL"
     gene            12393..12959
                     /locus_tag="KPD58_001983"
     CDS             12393..12959
                     /locus_tag="KPD58_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBG0265749.1"
                     /translation="MAYDTKKKISEQSQGIVAKIGIALGNVTSTLFQAGRDTIDTLIH
                     TILPFMAFVAVLIGIINQSGIGNWVANLLTPLIGNVGGLVVLSIICSFPLLSPFLGPG
                     AVIAQIVGVLVGTEIGKGNIPPSLALPALFAINSQAACDFIPVGLGLAEAEPDTVQVG
                     VPSVLYSRFLTGAPTVLLAWLASFGLYK"
     gene            13000..13365
                     /locus_tag="KPD58_001984"
     CDS             13000..13365
                     /locus_tag="KPD58_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBG0265750.1"
                     /translation="MELIYSTQINKIGDNANEFLQHNMFVTFKDNAPQELENYCYIHS
                     ENNLVKEILTDDVLSINNVDYKITSVGELVNQNLSELGHITFKFTGEKDGAIGGTLYL
                     EEKEIAPIGVGTILKIIRK"
     gene            13466..14239
                     /gene="srlD"
                     /locus_tag="KPD58_001985"
     CDS             13466..14239
                     /gene="srlD"
                     /locus_tag="KPD58_001985"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HBG0265751.1"
                     /translation="MRKVALVVGGGRSLGEYLSKHLAKVGYDVAIADLNEDNAKKVAE
                     EIKTEFGCKSVGYSCNAIDERQVISTVKAIENDFDRIDLLVYNAGVAVSKKITDFGYK
                     DFKFIVDVNLNGYFLFAREVSKVMIAKGIEGRIIQINSKSGRVGSKHNSGYSAGKFAG
                     VGLTQSLALDLAEHKITVNALMLGNLLDSDMFESLIPQYAVKLGIPESEVKQVYIDKV
                     PLKRGCRFIDVANMLTFYASKEAEYCTGQSINITGGQVM"
BASE COUNT         5554 a         1709 c         2437 g         4777 t
ORIGIN      
        1 taaattatgt aagtaaaaga ggggttgaat cagttgaaaa tgtaatatta gtaaatagta
       61 catcaatcct atataccctg taagcaactc tattttcatg tactaaaaat gtacctattt
      121 tattaactga aaagaataaa ttaaatgtaa gtacataaga agatagaaaa aattaaaaac
      181 aaaaaatatt tatataaata aatataaaaa atgatataat ccctattaaa aaatttatag
      241 atgatgaaga aatatcaaaa tcttatatta taggaagaga cacttctttg ccaaaatcta
      301 tagaaaagca ataccaaatc caaagagaat aggcgaagaa aaaaataaaa taaattcaag
      361 agtaatagaa aagttttata gaataaagaa ctgataactc tttatataac gaaaaataga
      421 attttaagag aaaatgattt aataaatgat ttaacatggt gttttagcag gtaaaaataa
      481 atcaccagta atgattatag aaaaattact ttatgaatct caaaaaactt agttaagagt
      541 aagagatttg ataagataac taaagttggg gaaatgtgtt tgatgagaca aaaaattagt
      601 aaaatagtat aatctaaata aggatatttt gttgattaaa ctacactaat agtaatattg
      661 tagggggaaa agatgagaga agaatcgaac tctttaagtt tagaccatat agaaattttc
      721 tttgaacttt tatctcaaag tacagaagat tatatatttt tttgggatat aaataggaat
      781 aaatttaaaa tttcttcagc aatttttgat gaatttaact taagtaaaga gatagaatct
      841 gatttggtca attgttggag taaaattgtt tatccggatg atgttcaaat atggaaagat
      901 gatatacaag aattactaca tggaaaaaaa ggtgagcaca atttagaata taggttgatt
      961 aataaatata aagaaatagt atggatttct tgtagaggta aagtttatgt gtcggatgac
     1021 ccaaaaacta tatttctagt cgggagaata aagaatattg gtgaaaaaaa taaatttgat
     1081 tctatcacag gaacgtggaa tagagaacaa tttgaacata gaatgaatta tcttataaaa
     1141 gagaaaattt ataaaaatgg tgctatgttt attatggata ttgataattt taaaaatata
     1201 aatgaaaaat atggacattc ttatggtgac aaagtattgc gtgcaattgc tactgaagta
     1261 ttagaatatc ttccaaaaga tgtaagattg tataggttag atggagatga atttgcattt
     1321 ttttacccta tgtgtactaa agaaactata gagaaaatat atgaaaagat acagatgtat
     1381 acaaatactc agcatgaaat tgaatcaaat aagtactatt gcacagtgac tgcaggcgtt
     1441 gctatgtatc cagaagatgg agataattat ttagatttat ttaaacatgc tgatattgca
     1501 ttggatatag caaaaattag tggtaaaaat agaattaagt ttttttcaca agagctctat
     1561 gaaaataaac taaaggtaat ttcaatgcaa caaaaactaa gagagtgcgt tgaaaataat
     1621 tttaatgact ttgagctgtt ttttcaaccc caggtaaatg ctgttacaaa agaggtaata
     1681 ggggcagagg tacttttaag atggcattcg agtacttatg gagaagtatc tcctgttgaa
     1741 tttataccaa tactagaaca gagtaattta attataccag ttgggaagtg gattataaaa
     1801 gaagctgtaa aacagtgtaa agaatggcat aaaataaatc cagactttaa aatatctgta
     1861 aatgtatcgt atattcagtt aaaggaagat ttttttagag attttatagt agaatgttta
     1921 gtagagtatc aattaagacc tgaattttta attttggaat taacagagaa ttgttggata
     1981 ccagatataa atttactaaa tgataaattt attagtttaa agggtatagg agtatatatt
     2041 gcaatagatg actttggtac aggatattca tctttaaatt accttaaaga actgtctgtt
     2101 aatataataa aaattgaaag aagttttgtt aagaatatta catataatag ttatgaatat
     2161 acttttcttg aatatattat taaattagct catattataa atttaaaagt ttgtgttgag
     2221 ggaatagagt cttatgaaga gtatgatata gtaaaaagct taggggtaga tattattcag
     2281 ggttttttat ttggaagacc tgtaagtgcc tctgaatttt ataaatttca actatcaaag
     2341 tgatgtatat aaaatgagat atatagtata taaaaatagg gcaaagtgga agttttgaag
     2401 tttattaaaa atttacactt taccctattt tgttatgtct tctataaagg attaatatac
     2461 tacactaact attatatact ttcactccaa atttagagat tatttcacta gctttttgga
     2521 catcttcctt agtggcatca ggaacatctt ttagttcata atcgatacct aaactttccc
     2581 atttgtgtat tcccatagaa tggtatggta gcaattcaaa tctttctaca tttttcaatg
     2641 tagctacaaa ttgacctagt ttttctaaat tatctatatc atcagtaatg ccaggaacta
     2701 aaacatgtct tatccataca ggaatatttc ttgattctaa gtgtctagca agttttaatg
     2761 ccttatccat accaacacca gtcaaatcaa tagatttttc ctcaaccata tgttttaaat
     2821 caagtaaaac caaatcagta ttatctaaaa ctgggtctat agtttctata tctacaaatc
     2881 cagatgtatc caagcaagtg tgtatatcat tttcttttgc ttttttaaat aattcactta
     2941 aaaattctgg ttgaagagtg gattcaccac cagaggcagt tatacctcca ccagaaaact
     3001 tcataaaaga agtatactta agagcatccg ttattatttc atcaactgtg tattcttttc
     3061 ctgatttaat atcccatgta tccctgttat gacagtattt gcatctaaga ggacacccct
     3121 gaaaaaataa tatatatctt attcctggtc catctacagt accaaacgtt tctatagaat
     3181 gtatctttcc cttaatcata gagttcacct cacaatatat tttaataacc ttatcaatag
     3241 ttataatatt tctctaaaaa aggccatctt ataccagaaa taattcaaat ataagatagc
     3301 cttttatttt tattttttac acagtagtaa tcttagttaa agtataaaaa tttaaagact
     3361 aagccatttt accgtggaat gttctgttta taacatctaa ttgttgctct ttagttaatt
     3421 ttatgaagtt aactgcatat ccagatactc ttatagttaa ttgtggatat tcttctggat
     3481 gttccatagc atcttctaaa gtagctctat caaatacatt tacatttaag tgatgagcat
     3541 tttgagcgaa gtatccatcc atcattgcag aaagattgtt tattctttca gtcatatctt
     3601 ttcctaaagc agcaggtact atagagaaag tattagatat accatcttga gcatgttcat
     3661 atggtaattt agcaactgaa gataatgaag ctaaagcacc attagtatct ctaccatgca
     3721 ttgggttagc accaggagca aatggagcac cagctcttct accatctggt gtattaccag
     3781 ttttcttacc atagacaaca tttgaagtta ttgtaagtat agattgagta ggataagaat
     3841 ttctataagt tttattcttt cttattttat tcataaagct ttcaactaac tctacagcta
     3901 tactatcaac tctatcatca ttatttccgt attttggata atctccttct atttcaaaat
     3961 caacagctat tccttgttca tttctaatag ttttaacctt agcatgtttt atagcagata
     4021 atgagtctgc acaaactgat aatccagcca taccacaagc catagttcta aatatatctc
     4081 tatcatgtaa tgccatttct aatgcttcat aagaatattt atcatgcata taatgtatta
     4141 catttaaagt atttacatat aatgttgcta accaatcagt gaatggttca aatttactca
     4201 taacttcatc ataatctaag tactcagaag ttataggttc aaatcttggt ccaacttgaa
     4261 caccagattt ctcatctaca ccaccattta tagcgtataa taaagtttta gctaagttaa
     4321 ctctagctcc aaagaactgc atttgtttac caattctcat tgcagataca caacaagcta
     4381 taccatagtc atctccccag tatgctctca ttaaatcatc attttcatac tgtactgaac
     4441 ttgtatctat agatacttta gaacagaaat ctttgaatcc ttgaggtaat ttagttgacc
     4501 atagaactgt taagtttggt tctggagaag gccctaatgt atatagagta tttaacattc
     4561 taaatgagtt tttagtaact aatgttctac catctattcc catacctcct atacactcag
     4621 ttacccaagt tgggtctcca gagaataagt tattgtaatc aggagttctt aggaacttaa
     4681 ccattcttaa tttcataaca aaatggtcca tatactcttg taattcttct tctgttacga
     4741 ttccagcttt taaatctctt tcaaagtata tatctaagaa agtagatgtt ctacctaaag
     4801 acattgcagc accattttgg tctttaacag cacctaaata accaaagtat aaccattgta
     4861 cagcttcttt tgagttagtt gctggttttg atatatcaaa tccataagat tcagccattt
     4921 ttttaagttc atttaaagct gatatttgct cagttatttc ttctcttaat ctgataactt
     4981 cctcatccat gcaagaaact tctaatgatt tcttttgctc atttttatct tctattaaag
     5041 catcaacacc atatagagca actcttctgt agtcaccaat tattctacct ctaccataag
     5101 catctggaag acctgttatt ataccagatt ttctagcagc tctcatttct ggagtataaa
     5161 catcaaaaac tccttggttg tgtgtttttc tgtatttagt aaatatatct tttatttctg
     5221 ggtctaattc atatccaaaa gcttcgcatg aattttcaac catctttata ccaccatatg
     5281 gcatgacagc tctttttaaa ggggcatccg tttgtaaccc aactattgtt tctaattctt
     5341 tatctaaata tcctggagca tatgcagcta ttccagaaac agtttttgta tcaacatcta
     5401 aagtaccacc attttctctt tcttttttaa ataggaccat tgcttcgtcc cataattttt
     5461 tagtattttc agtggcacca gctaagaaag agtcatttcc ctcatatgga gaatagttta
     5521 attgtataaa ctctctaaca tttatttctt tagtccatct tcctgtttta aatccttgcc
     5581 atgcattcat taagtatttc ctccttagat ttaaatgata aataatacta ttaaaattaa
     5641 tttttatata cttaatttat tttatatatt tttttaaaaa tatattttct aatataataa
     5701 tagcatatta ttcgtatcct gtatacataa aaaataaaaa attgtcgctt taaagtataa
     5761 aacagattat atgttaataa tgtggtgaaa ttatacccat atataaataa aataaaacaa
     5821 ataaatagct aattaaggag aaaatatttt ttattttttg tttaggataa ataaatttta
     5881 ttatggaaaa aagaaaaagc atacatcttc acaaactgaa tatttattta atagggggag
     5941 gtgatagatt gtttatcaca gtggtattat ttttagtagg tttcctatta attactaaag
     6001 gcgcagatat ctttataaat tgtactgtag agataggtaa aaaaacgaat atatcagaaa
     6061 ttatacttgg agctactata gttagttttg caacaacatt acctgaattt acagtgtctc
     6121 tacttgcttc tattgatggt catactacta tgagtttagg aaatgcagtt ggttctataa
     6181 tatgtaatac agggttagca ttagggttgg ttgtttttat aagtccattt aatgtagata
     6241 aaaaaatgtt tttttctaag tctttactat taatagtatc cgttatagtt ttaattttat
     6301 tgagtttaga tggagttata acaagaggtg attcattatt acttataata atattaatct
     6361 tttatatggt taataattat agaagtgtcg ttggaaaatc tgatactaaa aatagaaata
     6421 ttaaaaataa cactattaaa gacatatcta ctaaaaaagg gaaaaattat agaggttttt
     6481 ccgtattaga aattgtaaaa atactattac tttttgctac aggattgata atgatgataa
     6541 taggttcaca aatccttata gagagtggag taataatagc tagtttttta aatatacctc
     6601 aaggtatagt aagtttaact ataatagcac ttggaacttc tctgccagag atagtttcgt
     6661 cgattactgc tataagaaaa aatcatcatg aaatatcagt aggaaatatt ttgggtgcaa
     6721 acatattgaa tatagtatca gtaatagctg tatcagcaat accaaataac ataccaatac
     6781 tatctcaaaa tagacaattg gatataccat tcatgatatt attattatta atagtaataa
     6841 taccaacact taaatctaat aaacttagta gaatacaagg tatattgatg ttattcacat
     6901 attttctgta catatcaatt ttgtatttca tgtatattat ttaactatac tgtttaaaat
     6961 attaattgca gaatatccta aaaaagtata taatggatag gttaagatta attgataacg
     7021 gagaggaata atatatgaat ataacaaagt ttgaagattt accaataagt gagggaataa
     7081 aaaaagctat agctgaaatg gggtttgaag aaccatctcc tatacaagca cagtctatac
     7141 ctgcgatttt gtcaggtaaa gacgttatag gacaagctca aactggtacg ggaaaaacag
     7201 ctgcttttag tatccctata ctagaaacaa tagacccaaa taatagaagt ttacaagctg
     7261 tagtgctttg tccaacaaga gaactggcaa tacaagtatc aacagaaata agaaagttag
     7321 ctaagtattc gcacggaata aagacattac ctatatatgg tggtcagcca atagatagac
     7381 aaataaaatc tctaaagagt ggtgtgcaag ttgtaatagg tacaccagga cgtacaatag
     7441 accatatcaa tcgtaaaaca ttaaagatgg ataatgtaaa aatgattata ttagatgaag
     7501 cagatgaaat gctagatatg ggatttagag aagatataga aatgatttta agtaagattc
     7561 cagaagaaag acaaacaact ttcttttcag ctactatgcc aagaggaatt ttggaattaa
     7621 ctaagagata tcaaaaagac ccagagcata taaaagttgt tagaaaagaa cttacagttt
     7681 caaatacaaa gcaatactat atagaaacta ggtcatcaaa taaattagaa gtattatgta
     7741 gattagtaga tgtatatgac cctaaactat ctgttgtttt ctgtaatact aagagaaaag
     7801 cagacgaatt ggtaggagat ttacaagcaa gaggatattt tgcagatgca ttacatggtg
     7861 acttaaaaca gactcaaaga gatatagtaa tggataagtt tagaaatggt acaatagata
     7921 tacttgttgc tactgatgtt gcagctagag gtatagatgt tgatgatgtt gaatgtgtat
     7981 tcaactatga cttaccacaa gatgaagagt attacgttca tagaattggt agaactggtc
     8041 gtgctggtag agaaggtatg tcatttactt ttgtatttgg aaaagaaatg agaaagatga
     8101 aagatataga aagatatact aaatctaaac taataaagca taatatacca acaataactg
     8161 atgttgaaga gaaaaaagta ggtactttct ttgcacaagt taaacaaact atagaagaag
     8221 gtcacttaac taaacaatta caatggttag aaggtttctg taatgatgaa gattatgcaa
     8281 tggttgatat agctgctgca cttgtaaaac tatctctagg tgaagaaatg aaagaagaaa
     8341 taatagaaga aaaacctaga agagagcgtg gagatagaaa aggtggtact ggagctaaag
     8401 atggtatgat tagattattt atcaatattg gtagaaatca aagagtacaa gcaaaagata
     8461 tagttggtgc catagctgga gaagttggaa taccaggtaa agtagtaggt actatagata
     8521 tatatgataa gtatactttt gttgaaatac ctaaaaaaga tgcaaaaaca gtaattgaaa
     8581 agatgaaaga tattaagata aaaggtaata agattaatat agaaaaagca aataagaaga
     8641 aaaaataacg tatagtatac aaaactataa ttagaaaata tataaaataa tatattggat
     8701 atttaaaaag cctgatgtgt tttcaaaaca gatagtagat aatctgtaag agttaacagc
     8761 ataaggcttt tcttgtttta tatgatgtta taattttcca gattgttgtg aaaaatgtat
     8821 attagttcct ttttttcaaa tctgatttga aaaagagaat attaattatc tatatagttt
     8881 agattataat aaaaaatgta agataacaaa ggggagaaaa agatgtcaat gacttatatg
     8941 gaccctaggg tttttttgat tttagaaatg gtgttagaag aagacacaaa tactctagaa
     9001 caattttcaa atgttttggg tgttagtact aggactatca gaaactatat aaaccagatt
     9061 aatcgagaat taaaaggtgg tattgctaaa atagttaaaa atgaaaaagg tacatatagt
     9121 ataagaattg aggatgaaag taaattaatt gagattgtaa attttaatag aggaaagcta
     9181 tcaaatttta taaatttaaa ttcacctgat gaaagaataa attatgttct tgatatttta
     9241 actatgacca acaatccaat taccatagat gatttggctg aagagatatg tataggaaga
     9301 acaacattaa taaaggattt aaaaaaagca gacagaattt tagctgaata tgatttagaa
     9361 ttaaaaagga agccaaatat aggtatgatt ttagttggtg aagaaatgga tataagatta
     9421 ttaatccttg atagattgta tgaaaattat attgatgtct tggaaaacgt taatcagata
     9481 aatttagtta aagattatga tattgaatgt cttagagatg aattaaaaaa gttatttaaa
     9541 aaagaagaac tctacataac agaacaggtc ttgagagatg ttgaaagata tataatcatg
     9601 tctgttttaa gaaatctaaa tgggtacaaa tttgaaaaga tagataagag atttgaagtg
     9661 ataagatgtt cagatgaata tactttagga ttaaagctca aagctttatt ggaaaagata
     9721 tttaatatag ttttaaatga aaaagaaacc atatttttga caatgccatt aataggtaga
     9781 aaagctccta gtacaaaatt agcacttagc agtataaaga taaatgatag cgtaaaggaa
     9841 gttgtagatg aagtaactag ttttttatta gagacctcag ggattgattt taaagaagat
     9901 aaaaagctta tagaaaattt ggaatatcac ttgtattttg cacttaatag aatgagattc
     9961 aacataaggg taaaaaatcc tcttttacaa gaaataaagg aaagatatac atttccatat
    10021 agcgttgcaa agatagctgc aatgataatt gaggaaaagt ttgacttgaa aatatgtgac
    10081 cacgaaatag gatatatagc attacatttt ggaagttatt ttgaaaaaaa ttcaagaaaa
    10141 gttctaaatg taaatagggt agctgttgtt tgtggaacag gtcttgggac tgcacaactt
    10201 attagaataa agataggaaa aatacttgga gaaagtgtag aagtaaatac tttttctgat
    10261 atagaagcta aagctaattt agatttatta aagtattatg atttagtatt tacaacagta
    10321 gatttcaaaa cagacttaga tgttccaatt tttagagtaa atgcgttatt taatgaagaa
    10381 agtataaaga aagagataga aaatgcaata cttttgaaaa atagtataaa tcttaagtct
    10441 ataaaaaaga ttgaaaaaaa tatccctttt ataggtaaat taattgaaga ggataagttc
    10501 tttatattga ataagagtac gtttatggaa aatttagaag atatgttaga aaaattagtt
    10561 cataaaggaa ttattgatat agagttcaaa aaaaaggtat taaaaagaga aaagaaatct
    10621 tcaacagcat tagataatta tatagcattg cctcactcag ttaatactaa tggggaaaat
    10681 ctttatctgg catttggaat attagaaaaa ccagtgattt gggatacaaa ggaaattaga
    10741 ataataatat tgatgataat tccagataag aatgtagata gtacagacct gataataaaa
    10801 agttatgaag aagtacttga agtagggaga gataaaaaga tggtagaaga acttgctaag
    10861 gtaaaatcat ttgatgaatt ttataaaata attaccaaga ggaggtaata atatggaccc
    10921 agtaattttt attatagcat cacttattac agtgtattta ttaaatattt ttcttggata
    10981 cttacagctt aaagatttta ataaaaatta tattgaacta aaaagaaaag gaagagtggc
    11041 aattggcaga aaaaaaggaa gaatttcatc gggtacaatt gtgttgatac ttataaatga
    11101 tgatggtgtg attgtcgaga ctagaaaaat gcaaggtgtt acagtattgg caagagtaaa
    11161 gtcatttgaa ggtttggtag gaaagagctt agagtatata gaaaaatcag atttaactgt
    11221 ttacaataaa ctacttaaaa aagcaatact agatgcagtc aaacagtaca taacatttga
    11281 gaaaaataaa tgtgaagata caaataatct gagtactgat gaagagtgta ggatggaagt
    11341 agtttaaaaa aatatattta taaggaggag ttaatatgac gcaatttttt caagtattag
    11401 caagtggtgc agatagtttt atgaatttat ttagggcagg tgggcaacaa tttgtaggtt
    11461 ttgtaacaga tatagtacca cttttagtag cattattagt tacaatgaat gcaattatca
    11521 actttattgg tacagataga gtcgagagac ttgcaaaaaa atgttctgca aatatattta
    11581 ctagatattt gatattacca gtccttggta catttgtatt tgcaaaccca atgacactat
    11641 cattaggaag attcttgcca gaaaagttta aacctagtta ctttgcagca gcaagctttt
    11701 catgccatac aatgaatgga ttattcccac atatcaatcc aggtgaacta tttatatatc
    11761 taggaattgc aaatggtata acaactttgg gattctcaac agcagattta gcagttagat
    11821 atttgttagt gggtatagtt gcaaacttta ttaaaggtgt agttactgac tatacaacaa
    11881 aatttgtaga gaaacaacaa ggaatagcat taaattcaga tataaagatt ggataattac
    11941 tttaaggtat aaataaattt ttcaaataaa aagatagtgt atagaggagg ataaattata
    12001 tggcatttaa caaagttaaa atagtaaaag gttctggagg atggggtgga ccattgataa
    12061 tagaaccaac agaaaagaag aataaggtag tatatgtgac tgggggagca aagccagaaa
    12121 ctgcagtaaa aatagctgaa cttacaggat gtgagttaat agatggattt tcacagggtg
    12181 ttaaagatga tgaaatcgca tgtgtaataa taaattgtgg tggaacattg agatgtggta
    12241 tatatcctca aaagaaaata ccgactataa atataatgaa aacaggaaaa agtggaccat
    12301 tagcaatgtt tataaaagaa gatatatatg tttcaggggt aaaaccaaca aatgtagagc
    12361 agttataatt gaggtagtag gagggaagaa atatggctta tgatactaaa aagaagataa
    12421 gtgagcagag tcaaggaatt gttgcaaaaa taggtatagc actaggaaat gtgacaagta
    12481 ctctttttca agctggtagg gatactatag atacattaat acatactata ctgcctttta
    12541 tggctttcgt tgcagtccta ataggtataa taaatcaatc gggtatagga aattgggtag
    12601 ccaatttact tactccatta atcggaaatg ttggaggttt agttgtctta tcaataatat
    12661 gttcatttcc gttgttgtct ccgtttcttg gaccaggagc tgttatagct cagatagtgg
    12721 gagtattggt tggtacagaa ataggaaaag gaaatattcc accaagttta gcattaccag
    12781 cattgtttgc aataaactca caagcagctt gtgactttat accagtaggt cttggtcttg
    12841 cagaagctga acctgataca gttcaagtag gagttccgtc agttttatat tctagatttt
    12901 taacaggtgc accaactgtt ctattagcat ggttagcaag ctttgggtta tataaataga
    12961 agaatacttt ataaataatt agagagaggt tcagttgata tggaattaat atacagtact
    13021 caaataaata aaataggaga taatgcaaat gaatttttac aacataatat gtttgtaact
    13081 ttcaaagata atgctcctca agagttagaa aattactgtt atatacattc tgaaaataat
    13141 ttagtaaaag aaatacttac ggatgatgtg ctatcaataa ataatgttga ttacaaaatt
    13201 acttctgttg gtgagttagt aaaccaaaat ctttcagaat taggtcatat aacatttaag
    13261 tttacaggtg aaaaagatgg agcaattgga ggaaccttat atcttgaaga aaaagaaata
    13321 gcacctattg gcgtaggtac tatactaaaa ataattagaa aataaaattt aaaacgaatc
    13381 taatcatatt ataagtaaat tatagataaa agtcaattta actaatcaag ttaactttta
    13441 atgtgataaa aaaggggaaa tgaaaatgag aaaagtggct ttagtcgttg gaggaggaag
    13501 aagtttaggt gaatatttat ctaaacattt agctaaagta ggttatgatg ttgctattgc
    13561 agatttaaat gaagacaacg caaaaaaagt agctgaagaa ataaaaactg aatttggttg
    13621 taaatctgta ggatatagtt gtaatgccat agatgaaaga caggttataa gtactgtcaa
    13681 agctattgaa aatgattttg atagaataga tttattagtg tacaatgcag gagtggctgt
    13741 aagtaaaaaa ataactgatt ttggatacaa agattttaaa tttattgtag acgtaaattt
    13801 aaatgggtac tttctatttg caagagaagt atctaaagtc atgatagcaa aaggtattga
    13861 gggtagaata atacagataa attcaaaatc aggtagagtg ggaagtaagc ataattcagg
    13921 atattcagca ggtaaatttg ctggagtagg acttactcaa agtttagcgc tagatttggc
    13981 agaacataag ataactgtaa atgcactgat gctcggtaat ttacttgatt ctgatatgtt
    14041 tgaaagttta attcctcaat atgcagtaaa attgggaata cctgaatcag aagtcaagca
    14101 agtttatata gataaagtac ctttaaaaag agggtgtaga tttatagatg ttgcaaatat
    14161 gcttacattt tatgcttcaa aggaagctga atactgcact ggtcaatcta taaatattac
    14221 tggtggacaa gttatgtaag gttttatgga gtgtctcaag atgaactctt tagttcatga
    14281 ggcacttttt tgtataaaat taaatatatt ttcagcattt aaacaatgaa aaagaggttt
    14341 atctttattt tgagatagcc tcatttttgt aattaaaaaa catagtgttt aagtaaacat
    14401 ataagattgt tagtttttta aaagagaatt ttttaataat attatattta tgaagtattt
    14461 ttaaacttaa tttgata
//
LOCUS       DAELLV010000085        14394 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.085, whole genome shotgun sequence.
ACCESSION   DAELLV010000085 DAELLV010000000
VERSION     DAELLV010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14394
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.085"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            294..1712
                     /locus_tag="KPD58_001986"
     CDS             294..1712
                     /locus_tag="KPD58_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HBG0265752.1"
                     /translation="MTELMIISGLVLLICITSSKVLYKFGVPILLIFILLGMLFGSDG
                     MVGIYFDNYELTKQLCSIGLIFIMFFGGFGTNWNMAKPVAIPSILMSSLGVIITAGVT
                     GMFCHMVIGTSLLEGLLIGAIAASTDAASVFAVLRSQKLNLKGSLASLLEVESGSNDP
                     IAYMLTLILLTLMNNSGVELIAPMVIKQIAFGLAIGFILAKLSVYILRHSNFEIEGFY
                     TIFITAIAILSYAFSEYLGGNGYLSVYISGIIIGNSRIPLKKSLVHFFDGISWIMQIM
                     LFFMLGLLSFPSKLPNVFGIAMAISIFMIVIARPLATFIVLAKFKFSNKEKLFISWVG
                     LRGAASVVFAIYAVTQGVAIENDIFHIIFFIALLSVGIQGSLIPTVARKLDLVDDNTP
                     VLKTFNDYDGEISSKLIEVNVDEGNKWANKSIIDSNIPDDILIVMIKRKEQVLIPKGS
                     TIIKTGDILVLSGDRIEELIQL"
     gene            1788..2600
                     /locus_tag="KPD58_001987"
     CDS             1788..2600
                     /locus_tag="KPD58_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0265753.1"
                     /translation="MFSIGMFSKINKITTKTLRYYEDIGLLKPEYVDEFTKYRYYTTE
                     QLPKLHQIITLKQMGLSLAEIKKAVENPEEVENILMNKEKEMLDVIKSEEYKIIKLRN
                     YLMNLKGEVNMNDVIIKSLPKVKVASMRKVLKGYDELFNLFPNVMAEEMMKTGCTCAV
                     PDYCFNIYHDGEYRETDMDVEMCQAINDLKEDTDILKFKELDAVENAVCILHNGPYST
                     LGKTYADAFKWIEDNKYKVVGNPRESYIDGIWNKESEEDWLTEVQIPVKRML"
     gene            3023..3640
                     /locus_tag="KPD58_001988"
     CDS             3023..3640
                     /locus_tag="KPD58_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HBG0265754.1"
                     /translation="MEIFAIGHSNYPYDKLINMIKKYDINCVVDIRETPYSKYNIQYN
                     KEAFNESLRNSGFIYIYMGKEFGAKRTNKDVYTQEGYADFEKVAKEDIFLNGIERLKK
                     GCQMGYRIVLLGAMQEPIRCHRSILVGKVLNKEGFDVKYIMHEGNLAYQEDIEESLLD
                     KYFSDRKQLSIDNLLGSALTREEMIQEGYKLANKEIGYRTEKLGK"
     gene            complement(3797..4249)
                     /locus_tag="KPD58_001989"
     CDS             complement(3797..4249)
                     /locus_tag="KPD58_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staygreen family protein"
                     /protein_id="HBG0265755.1"
                     /translation="MVNLNANNLNVNMVYPISSTAPIRFRRYTVSYLENMENIYLTIA
                     TYFDTLGLGTMNIDKVYGQWAWFTDNIYDLNLFVFVGNYPYQIAKNRYDTFVDILPVA
                     ITSIVNGDRVFLISNPCLLNSKITVRFISSHPTLNRTVDYGNIRDFIF"
     gene            4697..6055
                     /locus_tag="KPD58_001990"
     CDS             4697..6055
                     /locus_tag="KPD58_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG0265756.1"
                     /translation="MFIRKNVIKLAIPIMIEQTFVMLLGVFNTMMAGHIGEEAVSAVG
                     MVDSINNIFISVFAALSVGATVVVAQQIGKKDSEKVNETIKQALVSGVILSFTITILM
                     WVFRTGVINLFYGSAEELVKSNAKLYIEFTLFTYPFIATQQIANGILRGCGDVKKPMY
                     VTMFMNIVNVTLGYILIYGIDLNPLGINLQTPSYGITGAAISIAIARIIGCIVIIRAL
                     FKGNEFISMGKIFPFKIDVETQKSIFNIGIPAGVEQLLFNAGKLIVQVFIVTMGTSAI
                     ASNAIGMSIASIINVIGNALALSATTLVGQYIGRGDIKGAKSTLLYLTKFATICLFIV
                     GAIFIPIAPWISSMYTQNASVIDISTQLIRSDCLAMVIWPISFVLSAGLKGAGDTRYT
                     MMTAVIGMWIFRIFTGYVLGVVLEFGVLGIWIGMYTDWLVRGALYCLRLRGTKWLKHK
                     VA"
     gene            6328..6708
                     /locus_tag="KPD58_001991"
     CDS             6328..6708
                     /locus_tag="KPD58_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG0265757.1"
                     /translation="MINKIGKITLYVNNQEDAKLFWTEKLNFVVTYEKEMGPNMKWVE
                     VAPSKNEFTTFVLYDKNLMMKQNKEINVSHPSIILSTRDINGTYEQMANNEVEVGELM
                     EMPYGRMFSFKDQDGNDYLVREDK"
     gene            6833..7033
                     /locus_tag="KPD58_001992"
     CDS             6833..7033
                     /locus_tag="KPD58_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265758.1"
                     /translation="MFKKMAVLKDIATKIGRKKAYELLEMVEGNDAFVAEVKIKKNGI
                     ESKKEEIMLKDNQKIILEYIEG"
     gene            7229..7957
                     /locus_tag="KPD58_001993"
     CDS             7229..7957
                     /locus_tag="KPD58_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0265759.1"
                     /translation="MLEIKNLSFNVESNNEELGIINDVSLSFERGKLIVITGPNGGGK
                     STIAKLIMGIEKATSGQIILDGEDITNLSITERAKKGIGYAFQQPPRIKGMTVENLLT
                     LAHGKPLSTDVCCQYLTDVGLCSKDYLNREVDNSLSGGEMKRIEIATLFARDLKVSIF
                     DEPEAGIDLWSFGKLNESFKKIHEESNQTIIIISHQERILELADEIIVLQDGSVKSHG
                     TKEAILPEIMCQVNSSCELMKDMN"
     gene            7979..8896
                     /locus_tag="KPD58_001994"
     CDS             7979..8896
                     /locus_tag="KPD58_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HBG0265760.1"
                     /translation="MNKIDLNLLEKVAELRGTPLGAYNIRKNGQGVERHSTENVTIIP
                     KTDKPGIDIIVKPNTKGENVHIPVIVTEAGVQDMVYNDFEIGENADVVIIAGCGIHNC
                     GCDDSEHNGIHRFFLRKGARLKYVEKHYGEGDGEGKRILNPVTIVNMDEDSYCEMETI
                     QIKGVDSTKREMDANLGKGAKIVVFEKLLTHGNQVAESNMKVILEGENSSAQVVSRTV
                     GQDNSKQKFNPCVIGDSLCSAHVQCDSIIMGNSQISAIPEIAANHPDALLVHEAAIGK
                     IAGDQILKMMTLGLTEEEAEQQILNCFLK"
     gene            9038..10222
                     /locus_tag="KPD58_001995"
     CDS             9038..10222
                     /locus_tag="KPD58_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG0265761.1"
                     /translation="MKNLLIRNGTIISPTDGIKFEKRDILVLHGKIYAIGLNLKEKIR
                     KEKLLTNKEFEIINADNMYISPGFIDVHTHCYKRKLPTGMDSDSIGIEKGSTVIFDAG
                     TAGPSNYYDFKKKYMDECKTEVYSFLNVTNEGLNILNESTSLDVINFDKVESIVEKNI
                     EKIKGIKVKASKFQSNESGIDIIKKSKQVAKKINLPLVVYIGEYPSYIDEVMNILGKG
                     DVVTPVYGNSTNGILKESGTLRKSVINAKNRGVLFDVCHGVDQFDFKVFKKAIKQGLE
                     PDLISTDMHIKNMKDVNYSLLNVINKIMELGISLEKCIEKVTEYPTKIFDLHGNKGRI
                     KIGGEGDFTIFTMEECNDIIKDYNNNELVLNKKLRLQYTVKSWMKSSEVYRHGYENTI
                     IN"
     gene            10329..10517
                     /locus_tag="KPD58_001996"
     CDS             10329..10517
                     /locus_tag="KPD58_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBG0265762.1"
                     /translation="MKAFVDKDVCVACGACIGTCPDVFSMSDEGHSVAIEGEIPEDLQ
                     DLCKEAEEGCPVSAITVK"
     gene            10666..11316
                     /locus_tag="KPD58_001997"
     CDS             10666..11316
                     /locus_tag="KPD58_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBG0265763.1"
                     /translation="MKKNYEIVLFDLDGTLTDPKEGITKSIQYSLNSFGIKEDLENLD
                     QFIGPPLHDTFKEYYKFEDKKAKEAVEKYREYFADKGIFENKIYENMKEILEMLYKNG
                     KILLVATSKPTVFAETILRYFDIDRYFKYIAGSNLDGTRVNKNEVIQYVLDLCNVKDK
                     DKVIMVGDRKYDIIGAKKIGIDSIGVLYGYGSFEEISESEPTYIVENVESIKDILL"
     gene            11419..12357
                     /locus_tag="KPD58_001998"
     CDS             11419..12357
                     /locus_tag="KPD58_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBG0265764.1"
                     /translation="MKSILIMGGSDFIGSALAKRLIKCGYQIDILTNGKKEIDYNGFK
                     EHLICDRKVRKDMENIITGRKYDYIYDMTAYTKEDVSNLIDFISMDNLKKYIVLSAGA
                     VYKDSGRNIKEENEKGENENWGKYGLNKKEAEDFIINSPIPYIIIRPTYIYGENNNLY
                     REYYFFEKIEKNEKIPVPKGKQVSNQFIYIGDLVKVLESIMKNPHVREAYNVTNPQLI
                     SWDDLIYTCGEIIGKEPIIKYVDMEKVEFRERTYFPFRNIDFNLDINKLIEHGLYIPN
                     VLLKEGLTATYKWFSANKPKMHDRKMNKVESVMQIV"
     gene            12479..13234
                     /locus_tag="KPD58_001999"
     CDS             12479..13234
                     /locus_tag="KPD58_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG0265765.1"
                     /translation="MKKYNISENGALIKNDTIESKSESVKVVQISDTQIGSFYSTKNL
                     KKVANKINTLNPDIIVFTGDLIDYSNKNPSVDEITTILSSMNARLGKFSVFGNHDYMY
                     KLPRYYRHIMKNSNFNLLVNENKKIKLKDDKYINILGVDEILNGNPNIKYLESQIDNK
                     SFNLLLAHEPDLVDMLSKDTMNLVLSGHSHGGQIRLPIKGALVTPPYGRKYTKGFYDI
                     NGNHLYVSSGLGSTKLPFRFFNIPEIIEFNIII"
     gene            complement(13519..14253)
                     /locus_tag="KPD58_002000"
     CDS             complement(13519..14253)
                     /locus_tag="KPD58_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBG0265766.1"
                     /translation="MSIQKLLSKARKAIQDFDMIQENDKIAVGLSGGKDSLTLLHILK
                     SYQRFSPQNFELIAITLNTGGVDNSPLDKLCKEINVPFYEFQTDIKEIVFDIRQEKNP
                     CALCANLRRGALNDNAKKLGCNKVALGHHKDDAIETFLMSMFYEGRVNCFSPKTYLDR
                     QDLTVIRPMVYIEEYMTKKISKDSNYPIITNPCPANGHTRREYIKNLIANLNKEMPDF
                     KRNVFGALNNSEKLFIWDKEKIKNFK"
BASE COUNT         5731 a         1426 c         2455 g         4782 t
ORIGIN      
        1 ctttatattt cgtagttata tataaaatta ctataaaaaa tgaaatatat taaacaatca
       61 actaaaaaat gaaatattaa taaaaaaaaa ttcaaattat aaatattaaa tgaatataat
      121 taaaacaaat atataaattt aagtataaca ctcacatttt aatgtattgt gaaatttttg
      181 ttgaacttta tgtaccataa cttataaaat ttatgtcatg ttatatttga ataagtaata
      241 aaattgtaga tgtaaattaa tctacaaaat ttatatgaaa tggagttgat gctatgacag
      301 aattaatgat tataagtggg ttagtactat tgatttgtat aacttctagc aaggtattgt
      361 ataaatttgg agtaccaata ttattgattt ttatcttact agggatgtta tttgggtctg
      421 atgggatggt aggaatctat tttgataact atgaactgac aaaacaatta tgttctattg
      481 gactaatatt tataatgttt tttggagggt ttggaacaaa ctggaatatg gctaaacctg
      541 ttgctatacc ttctatatta atgtcttcac tgggtgtaat aattacggct ggcgttacag
      601 gtatgttttg ccacatggta ataggaacta gtttacttga aggtttatta attggggcaa
      661 ttgcagcttc aacagatgct gcatctgtgt ttgctgtgtt gcgttctcaa aaactaaact
      721 taaagggttc acttgcatca ttattagaag ttgaaagtgg aagtaatgac ccaatagcgt
      781 atatgttgac actaattctt ttgacattga tgaataattc tggggtagaa cttattgcac
      841 caatggtaat aaaacaaata gcttttgggt tagctatagg atttatacta gcaaaattat
      901 ctgtttatat tttaagacat tcaaacttcg agatagaagg attttatact atatttataa
      961 cagctattgc aatactttct tatgcattta gcgagtatct tggaggaaat ggatatttaa
     1021 gtgtttacat ttcaggtatt attataggaa atagtaggat acctcttaaa aaaagtttgg
     1081 ttcatttctt tgatggaatt tcatggatta tgcaaataat gttattcttt atgttaggat
     1141 tgttatcttt cccttctaag ttaccaaatg tatttggtat agcaatggcc atatctatat
     1201 tcatgatagt tattgcaaga ccattagcaa catttatagt tctagcaaaa tttaaattct
     1261 caaataaaga aaaattattc atatcgtggg taggtcttag aggtgcggct tcagtagttt
     1321 ttgcaattta tgctgttact caaggagtag ccatagagaa tgacatattc catataatat
     1381 tttttatagc gttgctttca gtaggaatac aaggttcatt gataccaact gtagccagaa
     1441 agttggattt agttgatgat aatacacctg tattaaaaac atttaatgat tatgatggag
     1501 aaattagctc taaacttatc gaagtaaatg ttgatgaggg taataaatgg gctaataaga
     1561 gtataataga ttctaatata ccagacgata tattaatagt tatgataaaa agaaaggaac
     1621 aggttttaat acctaaaggt tcaaccatta taaaaacagg ggatattttg gtattgagtg
     1681 gagatagaat tgaagaatta atacaattat aatttatttt gtattgactt taacctaagg
     1741 ggaaagttta aacttaaatt aatcaaatct aatgcgcagg tgatgagatg ttttctatag
     1801 gaatgttttc taaaataaat aaaataacta caaagacatt aaggtattat gaagatatag
     1861 ggttgttaaa accagaatat gtagatgaat ttacaaaata taggtattat acaacagagc
     1921 aattacctaa attacatcag ataataactt taaaacaaat gggtttatca ttagctgaga
     1981 taaaaaaagc tgtagaaaat cctgaagaag ttgaaaatat tttaatgaat aaagaaaaag
     2041 aaatgttgga tgtaattaaa agtgaagaat ataaaataat taaactacgt aattacctta
     2101 tgaatttgaa aggagaagtc aatatgaatg atgtaattat taagtcttta ccaaaagtta
     2161 aagttgcttc tatgagaaaa gtgttaaagg ggtatgatga actttttaat ctatttccaa
     2221 atgtaatggc tgaagaaatg atgaaaacag ggtgtacatg tgcagttcct gattattgtt
     2281 ttaatatata tcatgatggt gaatatagag agactgatat ggatgttgaa atgtgtcaag
     2341 ctataaatga tttaaaggaa gatacggata tattaaaatt taaagaatta gatgcagttg
     2401 aaaatgcagt ttgtatatta cacaatggtc catattcaac attaggaaaa acttacgcag
     2461 atgcatttaa atggatagag gacaataaat ataaagtggt tggaaatcct agagaatctt
     2521 atatagatgg tatatggaat aaagagagtg aagaagactg gttgacagaa gttcagatac
     2581 cagtgaaacg catgttataa tatgtctaca aagtataaat aagaataaaa aaggtggtgt
     2641 ctcaaagtaa acattttagt ttatgagaca ctctttttca tatgaaatca agtatatttt
     2701 tataattacg attatgaaaa aaggcttatc tatattttta gacagcctct tattttttta
     2761 ttataatagt tttctaaaga tgctctattt ataaattttt attaaagact ttataaataa
     2821 ttgtatttaa tatacaaaat atatataaat attgatgttt tatatataaa gttgtatatt
     2881 tttttaagga tataaagtaa aaaaatatac aatatcaaga aaaaaagata ttgggtattt
     2941 aaagtaatac tttatttaat tttaagtaaa tcgggtatct acatatatag atactaattt
     3001 caaagaatag gagggtgttt atatggaaat attcgcaata ggacattcaa attatccata
     3061 tgataagctt attaatatga taaaaaaata tgacataaat tgtgtagttg acattagaga
     3121 aactccatat tctaaatata atattcaata caataaagag gcatttaacg aatctcttag
     3181 aaattccgga tttatatata tatacatggg aaaagaattt ggagctaaaa gaactaataa
     3241 agatgtatat acgcaagaag gttatgcaga ttttgagaaa gtagcaaaag aagatatttt
     3301 tttaaacgga atagaaagat taaaaaaggg ttgtcaaatg ggttatagga tagttttatt
     3361 aggagctatg caagaaccta taagatgtca tagaagtatc ttggtgggaa aagtattaaa
     3421 taaagaaggt tttgatgtaa aatatataat gcatgaagga aatttggcat atcaggaaga
     3481 tatagaggaa tcattattgg ataagtattt tagtgataga aagcaactga gtattgacaa
     3541 tttattaggg agtgcattaa cgagagaaga aatgatacaa gaaggttata agctagctaa
     3601 taaagaaata ggatatagaa ctgagaaact aggtaaataa aaatatacaa gtagataaaa
     3661 tacagtactt atcttaagta aaataatatt atgaatttat aatagctagg catgatgtgc
     3721 caattaaaaa tattctagtt atttaataat atcattaaat ttagggtatt ttttaatagc
     3781 acatcatgtt gcagttttaa aaaataaaat ctctaatatt gccataatca actgttctat
     3841 taagagtagg atgactagat ataaatctaa ctgtaatttt tgaatttaac aaacaaggat
     3901 ttgaaattaa aaatactcta tctccattga caatagaagt aatagctact ggcaaaatat
     3961 ctacaaatgt atcatatctg tttttagcaa tttgataagg ataatttcct acaaaaacaa
     4021 atagatttaa atcatatata ttatcagtaa accaagccca ttgaccataa actttgtcga
     4081 tattcattgt gcctaaacca agtgtatcaa agtatgttgc tatagttaaa taaatatttt
     4141 ccatgttttc taaatatgaa acagtatatc ttctgaaacg tatgggtgct gtggaagaaa
     4201 taggataaac catatttaca tttagattat ttgcatttaa attaaccaaa gtatcacatc
     4261 ctaaaattag tctataaaat acaatattta aatggatgcg attttgttac atctaaagtg
     4321 taatatttta tatattgaaa aagtggtaaa agtttggatg attaaataat attataaaaa
     4381 atatataaaa tgtacttatt gttattgcta cacgatagtg atacaatatt aacgaaatat
     4441 agctactttt aataataacg gaaaatattt atattaaatt aataaatatt ttaaatgtgg
     4501 tatccataaa aaataataaa cataagataa ttataaagat atactgtgtt caaatgatat
     4561 taaacaagat ttatattatt tgaaagcagt atattataat gcgaacgata caattctaaa
     4621 ctttgcattg tcaatttaca gttcgataaa ctaattaaaa attaaattta tataaagagg
     4681 gaaaagagga atagggatgt ttataaggaa aaatgttata aagttagcaa ttcctataat
     4741 gatagagcag acgtttgtca tgcttttagg agtatttaat acaatgatgg caggtcatat
     4801 tggggaagaa gctgtatctg cagttggaat ggttgattca ataaacaata tatttatatc
     4861 tgtatttgct gcactatctg ttggagctac tgttgttgta gctcaacaaa taggaaaaaa
     4921 agatagtgaa aaggtgaatg aaactattaa gcaggctcta gtgtctggag taatattatc
     4981 ctttacaata acaatattga tgtgggtttt tagaactggt gtaattaatt tattttatgg
     5041 ttcagcagaa gaattggtaa aatcaaatgc aaaattatac atagagttta cattatttac
     5101 gtatccattt attgcgacac aacaaatagc aaatggtatt ttaagagggt gtggagatgt
     5161 aaagaaacct atgtatgtga ctatgtttat gaatatagta aatgtaacat tgggatatat
     5221 acttatatat ggtattgatt taaatcctct aggaataaat ttgcaaacac catcctatgg
     5281 cataacaggt gcagctatat ctattgctat agctagaata attggatgta tagtaattat
     5341 aagagcttta tttaaaggaa acgaatttat atctatgggt aaaatatttc catttaagat
     5401 tgatgttgaa actcaaaagt ctatatttaa tataggaata ccagctggag ttgaacaact
     5461 actttttaat gcaggtaaat tgattgttca agtatttata gttactatgg gaacatcagc
     5521 aattgcatcc aatgcaatag gaatgtcaat tgcatccata ataaatgtaa ttgggaatgc
     5581 tttagctttg tctgctacaa ccttggtggg acagtatata ggacgtggag atattaaagg
     5641 agctaagagt actttattat atttaactaa atttgcaaca atttgtttat ttatagtagg
     5701 agctatattt ataccaattg caccctggat ttcaagtatg tatactcaaa atgctagtgt
     5761 aattgacatc tcaacacaat taattcgaag tgattgttta gctatggtaa tatggcctat
     5821 atcctttgta ttatcagcag ggttaaaggg agccggtgat actagatata caatgatgac
     5881 tgcagtaata ggtatgtgga tttttagaat ttttacaggt tacgtattgg gggttgtatt
     5941 agaatttgga gtgttaggaa tttggatagg tatgtatact gattggcttg ttagaggggc
     6001 tttatattgt ttaagattaa gaggaacaaa gtggctaaaa cacaaagttg catagaatat
     6061 atgaaatcac ttttagatgg ataactctat ttagaagtga ttttttatgt agttattatc
     6121 ataattataa gtatgatata taaggtgatt aaattacata aataaaggct ataatagtat
     6181 ggatattata aattatatat tgagcagaat tacataattt ggcgataaat attagaaata
     6241 tatatctata gaaaataata tatatgggta tattattaaa tataaaaaat attaggtatt
     6301 tatttaataa taaaggtggg tattgttatg ataaataaaa taggaaaaat aactctctat
     6361 gtaaataatc aagaggatgc aaagttattt tggacagaaa aattgaactt tgtagtaact
     6421 tatgaaaagg aaatgggtcc aaatatgaaa tgggtggaag tagcaccaag taaaaatgaa
     6481 tttacaacat ttgtgttgta tgataaaaat ctaatgatga agcaaaacaa agagataaat
     6541 gtatctcatc catcaataat tttaagtaca agagatatta atggaactta tgaacaaatg
     6601 gcaaataacg aggttgaggt tggggaatta atggagatgc catatggaag gatgttttct
     6661 tttaaagacc aagatggaaa tgattatttg gtcagagagg ataaataatt ttacaaagta
     6721 ttaaatttac tcaatatctg atataattag agacaataat tattataaac tagtctagga
     6781 ttagaattat ataattaaaa attaagtgta aattttgagt agaggtgata aaatgtttaa
     6841 gaaaatggca gtactaaaag atatagcaac taaaataggt cgtaaaaaag cgtatgaact
     6901 attagaaatg gttgaaggta atgatgcctt tgtagctgag gtaaagataa aaaagaatgg
     6961 aatagaatct aaaaaagaag aaattatgtt gaaagataat caaaaaataa tattagagta
     7021 tatagaaggt taaattttaa atacatataa gttgatatac taaatttctg agatgagttt
     7081 acataaaact catctttttt acttttcaat aactttgttt tattatttaa tatacatttt
     7141 atctccttag agttgctgat atataaaaat tactgatata ataaaagaat ggatattaat
     7201 gtaatatttt atagaaaaga ggggtattat gcttgaaata aaaaatcttt cctttaatgt
     7261 tgagtctaac aatgaggaac taggaataat aaatgatgtt agcctttcat ttgagagggg
     7321 aaaacttatt gttatcacag gtccaaatgg tggaggaaaa tctacaattg ctaagcttat
     7381 tatgggaatt gaaaaagcta cgagtggtca gataatttta gatggagaag atataacaaa
     7441 tcttagtatt acagaaagag caaaaaaagg aataggatac gcatttcaac aaccaccaag
     7501 aataaaaggt atgacagttg aaaatttact tacacttgct catggaaaac cattgtctac
     7561 tgatgtatgt tgccaatacc ttacagatgt tggattgtgt tcaaaagact atttaaatcg
     7621 tgaagttgat aactctcttt ctggagggga aatgaaaaga attgagatag caactctttt
     7681 tgctagagat ttaaaggttt ctatatttga tgaacctgaa gcagggatag acctttggag
     7741 ctttggaaaa ctcaatgaaa gttttaagaa aatacatgaa gagtctaatc agactataat
     7801 cataatttct caccaagaaa gaatattaga gttagctgat gaaataattg ttctacaaga
     7861 tggttcagta aaaagccatg gaacaaaaga ggctatatta cctgaaatca tgtgtcaagt
     7921 taattcaagt tgtgaattaa tgaaagacat gaattaatta gaaaggaagg caaattaaat
     7981 gaacaaaata gatttaaatc ttttagaaaa agttgctgaa cttcgtggaa ctccattagg
     8041 agcatacaat atacgtaaaa atgggcaagg agtagagaga cattcaactg aaaatgtaac
     8101 tataattcct aaaactgata aacctggaat agatattata gtaaagccaa atacaaaagg
     8161 agaaaatgta catataccag taatagtaac agaggctggt gtacaggata tggtatataa
     8221 tgattttgaa attggagaaa atgcagatgt tgttataatt gctgggtgtg gtatacataa
     8281 ctgtggttgt gatgatagtg aacataatgg tatacataga tttttcttac gtaaaggagc
     8341 tcgcctaaaa tatgtagaaa aacattatgg tgaaggtgat ggagaaggaa agagaatttt
     8401 aaatccagtt actattgtca atatggatga ggattcatat tgtgaaatgg aaacaattca
     8461 aattaagggt gtagattcaa ctaaaagaga aatggatgca aacctaggta aaggagctaa
     8521 aatagttgta tttgagaaat tattaactca tggtaatcaa gttgcagaat ctaatatgaa
     8581 ggttatctta gagggtgaaa actcttctgc tcaagtagtt tctcgtacag ttgggcaaga
     8641 taattctaag caaaagttta atccttgtgt tattggggat tccctttgtt ctgcacatgt
     8701 tcaatgtgat tcaataatta tgggaaattc tcaaattagt gctatacctg aaattgctgc
     8761 aaatcatcca gatgctcttt tagtacatga agcagctatt ggaaagattg caggagacca
     8821 aatactaaag atgatgacat taggtcttac tgaagaagaa gccgagcaac aaatacttaa
     8881 ttgcttctta aagtaaatta aagattttat ttaatttaat ataattaata tagtataata
     8941 tagtgaataa gtctaaaata tttttatttt taggcttatt ttctttatgt aagaaaagct
     9001 attagagaaa ttatattgta attaggatgt ggaataaatg aaaaatttac ttataagaaa
     9061 tgggacaata atatctccaa cagatggtat caaatttgaa aaaagagata tcttggtttt
     9121 acatgggaaa atatatgcta ttggattgaa ccttaaagag aaaataagaa aagaaaaatt
     9181 acttacaaat aaagagttcg agataataaa tgccgataat atgtatattt ccccaggatt
     9241 tatagatgtt cacacccatt gttataagag aaaattacct acaggtatgg attctgattc
     9301 aattggaatt gaaaaaggtt ctacagttat atttgatgca ggtacagctg gtccttcaaa
     9361 ctactatgat tttaaaaaaa agtatatgga tgagtgtaaa acagaagtat attctttttt
     9421 aaatgttaca aatgagggac taaatatatt gaatgaatca acttctttag atgttataaa
     9481 ttttgacaag gttgagtcaa ttgtagaaaa aaatatagaa aagataaagg gtataaaagt
     9541 aaaagcgagt aaatttcaat ctaatgaaag tggtatagat ataattaaga aatcaaagca
     9601 agttgctaaa aaaataaatt tacccttagt ggtatatata ggagagtatc catcatatat
     9661 agatgaagta atgaatattt tgggaaaagg agatgtggtt acaccagtat atggaaatag
     9721 tacaaatgga atccttaaag aatctggcac attaagaaaa tcagtaataa atgcaaagaa
     9781 tagaggagta ttgtttgatg tatgtcatgg agtggaccaa tttgatttta aagtttttaa
     9841 aaaagcaata aagcaaggtt tagagcctga cctaataagt actgatatgc atataaaaaa
     9901 catgaaagac gtaaactatt ctcttttaaa tgttataaat aaaattatgg aacttgggat
     9961 atccctagaa aagtgtatag aaaaagtaac tgaataccca acaaagattt tcgatttaca
    10021 tggaaataaa ggcagaataa agattggtgg agaaggtgat tttacaatat ttacaatgga
    10081 ggagtgtaat gatattataa aagactacaa taataatgaa cttgttttaa ataaaaaatt
    10141 aagattgcaa tatactgtaa aatcatggat gaaaagcagt gaagtttata gacatggata
    10201 cgaaaatact ataataaact aaacaatagt gataaaataa taaatatatg tttaaattgg
    10261 tatatattgg ataaatatct aaggaaacaa caattaaaaa ataataattt taggggaggt
    10321 attttagtat gaaagcattt gttgataagg atgtatgtgt agcttgtgga gcatgtatag
    10381 gaacatgtcc agacgttttt agtatgagcg atgaagggca ttcagtagca attgaaggag
    10441 aaataccaga agatttacaa gatttatgta aagaagccga agaaggatgt ccagtttcag
    10501 ctataacagt taaatagagc tgtttaaatt tatttttaaa ataaatatat aagatttaat
    10561 tggtacttaa gctatagtat tagaaatttc tattactata gctttttaaa atagataaac
    10621 ttaatatata agatattact ttttacaaat atggaggact gaaatatgaa aaaaaattat
    10681 gaaatagtat tattcgattt agatggaaca cttacagacc ctaaagaagg aataacaaag
    10741 tctattcagt attctttaaa tagctttggc ataaaagaag atttggaaaa cttagaccaa
    10801 tttataggac caccattaca tgatactttc aaggagtatt ataaatttga agataaaaaa
    10861 gctaaagaag ctgttgaaaa gtatagagaa tattttgctg ataaagggat atttgaaaat
    10921 aaaatttatg agaatatgaa agagatatta gaaatgctct ataaaaatgg taaaatatta
    10981 ttagtagcaa cttctaagcc aacagttttt gcagaaacta tacttagata ttttgatata
    11041 gatagatatt ttaaatatat agcaggtagc aatcttgatg gtacaagagt gaataaaaat
    11101 gaagttatac aatatgtact agacctatgt aatgtaaaag ataaagataa agttattatg
    11161 gttggagata gaaagtatga cataattgga gctaaaaaaa taggtataga ttctattgga
    11221 gtattgtatg ggtatggttc ttttgaagag attagtgaaa gtgaacctac ttatattgtc
    11281 gaaaatgtag agtctataaa agatattttg ttataaatat cttttataga caaaaatatt
    11341 acaaaatggt gtcgcatatt taaaaatatg atattatagt taaggaagtc atttaaaaaa
    11401 gaggaggatg cacttaaaat gaaatctatt ttaattatgg gtggaagcga cttcataggt
    11461 tctgcacttg ctaagcgctt gattaaatgt gggtatcaaa ttgatatact tacaaatggt
    11521 aaaaaggaaa tagattataa tggttttaaa gaacatctaa tttgtgatag aaaagttcga
    11581 aaagatatgg aaaacattat cacaggaaga aaatatgatt acatatatga tatgacagca
    11641 tatacaaaag aagatgtaag taatctgatt gattttataa gcatggataa cttaaaaaag
    11701 tatatagtat tgtcagcagg agcagtatat aaagatagtg gaagaaatat aaaagaagaa
    11761 aatgagaaag gtgaaaatga aaactgggga aaatatgggt taaataaaaa agaggctgaa
    11821 gattttatta taaatagccc tataccatat ataattatta gaccaactta catatatggt
    11881 gaaaataata atttatatag agagtattat ttctttgaaa aaatagaaaa aaatgaaaag
    11941 ataccagtac caaaaggaaa acaagttagt aatcaattta tatatattgg ggatttggta
    12001 aaggtattgg aaagcataat gaaaaatcct catgtaagag aggcctataa tgtgacaaat
    12061 ccacagttaa tttcttggga tgaccttata tatacatgtg gagaaattat tggcaaagaa
    12121 cctataataa aatatgtaga tatggaaaaa gtagagttta gagaaagaac gtatttccca
    12181 tttagaaata tagactttaa tcttgatata aataagctta ttgaacatgg gctttatata
    12241 ccaaacgtgt tactaaaaga aggtcttaca gcaacatata aatggttttc agcaaataag
    12301 ccaaaaatgc atgacagaaa aatgaacaaa gttgagtctg taatgcaaat agtttaaaaa
    12361 aataaataag taggtagata ttttgaactc aaaaattaag tattttttaa aattttttat
    12421 cttaatattt attttgattt ttatatactc agttgcgata gagccgagtc tattaattgt
    12481 gaaaaaatat aatataagtg aaaatggagc attgataaaa aatgatacaa ttgagagtaa
    12541 atctgaaagt gtaaaagtag ttcaaatttc agatactcaa ataggtagtt tttattcaac
    12601 taagaatctc aaaaaagtag ctaataagat taacactttg aatccagata taatagtatt
    12661 tactggtgat ttaattgatt actccaataa aaatccaagt gtagatgaaa ttacaactat
    12721 tttatcaagt atgaatgcta ggttgggaaa attttcagta tttggcaatc atgattatat
    12781 gtataaactt cctaggtatt ataggcacat tatgaaaaat tccaacttca atcttttagt
    12841 aaatgaaaat aaaaaaataa aattaaaaga tgataaatat attaatatat taggtgttga
    12901 tgaaatatta aatggaaatc ctaatataaa atatctggag agtcaaatag ataataaaag
    12961 ttttaattta ttgttagcac atgaaccaga tttagtagat atgcttagta aagatacaat
    13021 gaatttagtt ctatctgggc atagtcatgg tggtcaaata agattaccta ttaagggagc
    13081 attagttact cctccatatg gaaggaaata tactaaaggg ttttatgata taaatggaaa
    13141 tcatttatat gtgagttcgg gtcttggaag tacaaagtta ccatttagat tttttaatat
    13201 accagagata atagaattta atattataat ctaacataaa atatttagaa aatataattt
    13261 tgctttttat aatatgtaga tgtatatact gtaaaaatgg attaaaccag tagattgaat
    13321 gaattaatag attaaattaa tatataaaat atttaagtta taattaatgt attagacaaa
    13381 ataaatacta ttaagaacta ttaaaatata gattaaagat aataacatat aataaataaa
    13441 aaagtaagat tgtgtagatt atataaagat tatttacaat taatacaatc ttgctttttt
    13501 atttaaaaat tttaattatt atttaaaatt ttttatcttc tctttatccc atataaacaa
    13561 tttctcagaa ttatttaaag caccaaatac atttctttta aaatcaggca tctctttatt
    13621 taaattagca ataagatttt ttatgtactc tctcctagta tgaccatttg cagggcaggg
    13681 atttgtgata ataggatagt tagaatcttt tgaaattttc ttagtcatat actcttctat
    13741 gtaaaccata ggtcttataa ctgttaaatc ctgtctgtct aggtatgtct ttggggaaaa
    13801 acaatttact ctaccctcat aaaacataga cattaaaaat gtctcaattg catcatcttt
    13861 atgatgacca agagctactt tattacatcc aagtttttta gcattatcgt tcaaagcacc
    13921 tcttcttaaa ttagcacata atgcacaagg attcttttct tgtcttatat caaatacaat
    13981 ttcttttatg tcagtttgaa attcataaaa aggaacattt atttctttac acaatttatc
    14041 tagtggagaa ttatcaacac ctccagtatt taatgttatg gctatcaatt caaagttttg
    14101 tggagaaaat ctttgataac ttttaagtat atgaagtaaa gttaaactat cttttccacc
    14161 agaaagtcca accgcaattt tatcattttc ttgtatcata tcaaaatctt gtatagcctt
    14221 acgagcttta cttagaagtt tttgtataga cattttatca ccatccttta aaaatattaa
    14281 atttaagatt tatttaagat ttacttataa aatacttaag tcttatactc taaaattaac
    14341 attatagcaa acataacaaa aaggagaaga tttaattatg aaaaaattta aatt
//
LOCUS       DAELLV010000086        14061 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.086, whole genome shotgun sequence.
ACCESSION   DAELLV010000086 DAELLV010000000
VERSION     DAELLV010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14061
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.086"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..250
                     /locus_tag="KPD58_002001"
     CDS             <1..250
                     /locus_tag="KPD58_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265767.1"
                     /translation="PTTDKTREKFSKIKLNEIKKIYETIGINSITKISIDKNKLEMEG
                     VCSDLDVLNNLRKIKYIKYYYIDSIVRDGEYYRFRIKS"
     gene            265..849
                     /locus_tag="KPD58_002002"
     CDS             265..849
                     /locus_tag="KPD58_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265768.1"
                     /translation="MRLNNREKNLLKILLILVIILASDIAVSYLEENLTSLKNEKQNI
                     INKINNQDNYQIKDNKHIDDKYIESVESNKNEELKNNFNNSFLDKKTNKDIVLLVENE
                     LRDIVNVEYINQESSLDNHNKKQVNLEIKVNGELANVLKLEGVMKKLRLNNKLEKIEI
                     NKLKVNNFDVQNIPNEYKQEKDNIVECKMTVRVI"
     gene            1102..2139
                     /locus_tag="KPD58_002003"
     CDS             1102..2139
                     /locus_tag="KPD58_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HBG0265769.1"
                     /translation="MLGRNELCPCGSGKKYKKCCLNKDIVSERACRKTELSQKQYTDL
                     YSKLYDYSRQDKFKEECEKAKEMFYIMQNDSVNEKFERFFNTYLIQDHIMENKKVMTV
                     GFLEENGTNLSQSEVSILKSMFESYVSIYEVKEVSAGKIVLKDCLSGNELCTEDVKLL
                     RSFKIGSCMIARIVDIEGTNILIDITISISNEVKDIILKDIMNLFNQYQDVYKDMKSF
                     LIYHTHILYKYIQQLLDPSIAEYLKRERDKNDTKVNSENELAEEECKVLDTLKENVEK
                     ENIDACIEFWNEYKKNHENIKGAENGWAAAVEYYVKKEAGQPITQVQISKKYDISSST
                     LGKRYKDLKIS"
     gene            2220..2585
                     /locus_tag="KPD58_002004"
     CDS             2220..2585
                     /locus_tag="KPD58_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265770.1"
                     /translation="MSLSLGNAIAVSTNFIKSNPAVPVYNGEEIYAVIYHIEIGYNNG
                     VSPDTKQYIIIDYKEFDLSMDKKNELINIAKEYCKSKGIVENENLEINLLDNEEAENF
                     LEKVFNNMKVIRGLITKKG"
     gene            2590..2907
                     /locus_tag="KPD58_002005"
     CDS             2590..2907
                     /locus_tag="KPD58_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265771.1"
                     /translation="MDYIGIENITPYENTYEFSVYEYDDEITLGSEKLYVCELRVVLI
                     KVNSLYVERLHKSVEAMVLVKNLKKDLDKTLVVNKIKNFVLDEIWVENLVKENIEVIF
                     VES"
     gene            2955..4559
                     /locus_tag="KPD58_002006"
     CDS             2955..4559
                     /locus_tag="KPD58_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265772.1"
                     /translation="MLRVSNVKLGIDKDISLLKDVILKKLKIKEKNLVKYSIYKESID
                     ARKKGKMEFVYSVDVEVKDENKILNSKIKDVTKIKEIRYVNVPVGDKKLKNNPLVIGS
                     GPAGLFAGLLLAQMGYEPIILERGLDVDSRTKDISDFWTTRKLKNNSNVQFGEGGAGT
                     FSDGKLTTRIKDIRCRKVLEELVQFGAPDEILYSHKPHVGTDILKNVVKNIRNEIISL
                     GGQVRFNSKVTDIVVSNDSIQSVIINDTEKIDTDTIILAVGHSARDTYEMLYSRGIKI
                     VQKPFAIGARVEHPQDLINKAQYKDFYNHPRLGAADYRLIEHVSNGRTAYTFCMCPGG
                     SVIASASNDFEIVTNGMSEHARDKINANSAFLVNVTPEDFKSDNSLAGVHFQQKYEKL
                     AFELGGKNYNAPVQLVGDFINNKTSLNLGSVEPSYRPGYTLTNLSECLPSFVTETMRE
                     AFISLDKKLNGFAMYDAVLTGVETRSSSPIRIVRDENTLESVSIKNLYPCGEGAGYAG
                     GIVTAAVDGIKCAEKIIQKYTNNTKI"
     gene            4683..6311
                     /locus_tag="KPD58_002007"
     CDS             4683..6311
                     /locus_tag="KPD58_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBG0265773.1"
                     /translation="MNIIISLMGGLGLFLYGMNLMGEGLQKSAGTKLKKIIKLLTSNL
                     FMGVLVGTGVTAVIQSSSATTVMVVGFVNAGIMTLKQAIGVIMGANIGTTVTAQLVSF
                     DLTGMAPVALGVGIILYLFGNKPRIKNIAEILIGFGILFTGMDFMKMAVEPLRDYQGF
                     TDLLVTFGRYPLLGLLLGFGITAIIQSSSASMGMLVALAAEGLVPLSAALPILYGQNI
                     GTCVTSLLSSIGANKNARRAAMMHLIFNVLGTVIFLIFLNKPVVSMVTSWDPSNVARQ
                     IANTHTLFNIISVLILLPFTNLIIKLAIKLVPDRAGDIDEDETKTIKYIDDRMIETPS
                     IALANTIKEALRMGEKAKESLNASMEALVEHSTEKIDKTYRRERLINDLQKAILNYLL
                     KLSKAPLDDDSREVVDTLFNTVNDIERIGDHAKNIAELSQVAIDSNISFSEEGQSELD
                     VMYNRVVSAYTYALESMRTDNVDLACKVIKIEEQVDIMEKSCRANHMYRLNNNLCSIE
                     NGVIYLDVISNLERISDHAVNIAQQVIAKRLGND"
     gene            6576..7925
                     /locus_tag="KPD58_002008"
     CDS             6576..7925
                     /locus_tag="KPD58_002008"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBG0265774.1"
                     /translation="MGKINFDYSKATEFFCQNEIDVMQPYVDVAHDMLHNKTGLGNTF
                     LGWIDLPKNYDKEEFDRIKKSAEKIKSDSDVLLVIGIGGSYLGSRAAIDMVSHSFRNG
                     LKKEQRKAPEVYFVGHNISSTYIMDLLDIIEGKDISVNVISKSGTTTEPALAFRIFKD
                     YLEKKYGKEEARKRIYATTDASKGALRQLATEEGYETFVIPDDVGGRFSVLTAVGLLP
                     IAAAGLDIDAMMKGANDAREAFQNPDLKSNDSYRYAVARTILHRKGKDVELLVNYEPQ
                     LHYVSEWWKQLYGESEGKENKGLFPASVDFSTDLHSMGQYIQDGKRLLFETVLNVENC
                     KRNITISSEEVDLDGLNYLAGKTVDFVNHKAFEGTLLAHTDGKVPNLVINIPQLDEYN
                     FGYLVYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLGKPGYEKEKEELEKRLK"
     gene            8081..9160
                     /locus_tag="KPD58_002009"
     CDS             8081..9160
                     /locus_tag="KPD58_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBG0265775.1"
                     /translation="MSRRKKGISLVILVAIISSILSSFLTIILVKDNLVSKSTGSSTP
                     IVVNDDGKSQNIYQAVAEKATPSVVGITTTSVDTSNMFAIPTETQGVGTGIIVDSNGY
                     ILTNSHVISDGQATSVNVLFNDGSTTSGKVVWFDQQLDLAIVKVDKTGLTPAEFADSD
                     KVKVGDISIAIGNPLGLDFQKTVTQGIISGLDRTIQTEKTNMTGLLQTDASINAGNSG
                     GPLLNQKGQVIGINTAKASQAEGLGFAIPINTAKSIVEEVIKNGKYEKVTLGIKGTDV
                     SNYEAATGTKLSTDKGVYVAEVISGSSAEKAGVKVGDIITKVGDTDITGMNDLNKKLY
                     TFSKGASTKITVNRGGKAVTINVNF"
     gene            9505..10413
                     /locus_tag="KPD58_002010"
     CDS             9505..10413
                     /locus_tag="KPD58_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBG0265776.1"
                     /translation="MNPLVINLQKCSIHDGPGIRSTVFFKGCPLECVWCHNPESQTYT
                     KQVLYNEERCSKCEACINICPHKAIYKGETKICLDQDKCEFCETCLDYCVNNAREIVG
                     QEYSVRDLVKEIEKDRIFYEESGGGVTLSGGEVMAQDMDFICGVINMCKSKGIHVAID
                     TCGYAKSENYERVAKCADLFLYDIKLIDEDKHIKFTGKSNDLILKNVKILSELGANIN
                     IRIPLIVGVNVDDENLEVKKMIEFLKPLNIQAVSLLPYHNIGKHKYDKIYKKYEGEEL
                     QRPSEEKLEEIKRLFEASNFNTKIGG"
     gene            10418..12787
                     /locus_tag="KPD58_002011"
     CDS             10418..12787
                     /locus_tag="KPD58_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HBG0265777.1"
                     /translation="MARGTFERTKKLREESINAEPHISIERAVLMTEAYKKYEGSVEI
                     PVLRALSFKHYIENRTLSINDGELIVGEKGDSPNGAPTYPEICCHTMEDLEVMHNRDI
                     INFSVSEEARKIHKEEIIPFWKKRQTRDKIINAMTPEWLAAYEAGMFTEFMEQRAPGH
                     TVCGDTIYKKGFLDLKKDIEARLKELDFLNDLDAYNKKADLEAMAIACDAMVILGKRY
                     AEKARQMAEEETDEAKKKDLLLIAETCDVVPAHKPETYHQAIQMYWFVHIGVTTELNI
                     WDAFTPGRLDQHLNPFYERDVENGILDRDRAQELLECLWVKFNNQPAPPKVGITLKES
                     STYTDFANINTGGINPDGQDGVNEVSYIILDVMDEMKLIQPSSNVQISKKTPQKFLKR
                     ACEISRKGWGQPAFYNTEAIVQELMEAGKTIEDARLGGTSGCVETGCFGKEAYVLTGY
                     MNIPKILELTLNNGYDPISKKQIGIETGDPRNFQSYEELFEAFKKQLHYMIDIKIEGN
                     AVIENICAKHMPCPLMSTIVDDCIEKGKDYQRGGARYNTRYIQGVGIGTITDSLTAIK
                     YNVFDKKKFDMDTLLKALDANFEGYEAILNLVSNKTPKYGNDDDYADEIMQEIFNAYY
                     NEVTGRPTVCGGEYRVDMLPTTCHIYFGEIMGASPNGRLCAKPVSEGISPEKGGDTNG
                     PTAVIKSCAKMDHIKTGGTLLNQRFAPSVVQGEKGLDNMANLVRAYFNMDGHHIQFNV
                     FDKNVLLEAQKNPQDYKDLIVRVAGYSDHFNNLSRTLQDEIIGRTEQTF"
     gene            13112..13915
                     /gene="proC"
                     /locus_tag="KPD58_002012"
     CDS             13112..13915
                     /gene="proC"
                     /locus_tag="KPD58_002012"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBG0265778.1"
                     /translation="MKTLGFIGSGNMGSAMIGGIVQSELVKAEDITVSDLSQVALDKV
                     KEEFGVNTTTDSKVAVTNSDVVIVALKPNICEKALTPLKELIDANKIIVSIAAGKTIE
                     SLESFIGSDKKIVRVMPNTPALVGEGMSALCKNSNVTDEELNMIKALFESFGEAEIVS
                     EYLMDTVTGISGSSPAYVFMFIEAMADAAVLAGMPRQQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPAGTTIEAVKVLEEEGFRAAVIKSIVACIDKSKDMSK"
BASE COUNT         5753 a         1455 c         2601 g         4252 t
ORIGIN      
        1 accaacaaca gataaaacta gagagaaatt ttctaaaata aaacttaatg aaataaaaaa
       61 aatatatgaa acaattggaa taaatagtat tactaaaata tcgatagata aaaataagct
      121 agagatggaa ggtgtgtgta gtgatttaga tgttttaaac aacctaagga aaataaagta
      181 cataaaatat tattacatag acagtattgt aagagatgga gaatattata gatttagaat
      241 aaaaagttag atgggtgatt atttttgaga ttaaataata gagaaaaaaa tctacttaaa
      301 atattgttaa tattagttat tatattggca agtgatatag cagtaagtta tttggaagaa
      361 aacctaacta gtttgaaaaa tgaaaagcaa aatataataa ataaaataaa taatcaagat
      421 aattatcaaa taaaggataa taaacatata gatgataaat atatagaaag tgttgaatca
      481 aataaaaatg aagaattgaa aaataatttt aataactcat ttttagataa aaaaacaaat
      541 aaagatatag tgttattagt agaaaatgaa ctgagagata ttgtgaatgt agaatatatt
      601 aatcaagaaa gtagtttaga taatcacaat aaaaaacaag ttaatttaga aataaaagtt
      661 aatggagaac ttgcaaatgt gttgaaactt gaaggtgtaa tgaagaagtt aagactaaat
      721 aataaacttg aaaagataga aattaataaa ttaaaagtca ataattttga tgttcaaaat
      781 attccaaatg aatataaaca agaaaaagac aatattgttg aatgtaaaat gacagttaga
      841 gtcatataac aaaaaagaat agataaagta aaaaactatt atcaattaat aaaattttat
      901 agaggaatta gttttacgat aattcatttc tacaagaggg attgttgatg attttatcga
      961 aatcctaatt aaagttaata tattgtacca aaatataagt gaatttaata gaatatttac
     1021 ttatactata gattgattaa taaaaaaatg gtataataac aaattgtact ttaatttaat
     1081 aataagggag tggaagtcat attgttaggg agaaatgagc tatgcccttg tggaagtggc
     1141 aaaaaataca agaaatgttg tttaaataaa gatattgttt cagaaagagc atgcagaaaa
     1201 actgaacttt cacaaaaaca atatacagat ttatattcaa agttatatga ttattcaaga
     1261 caggataagt ttaaagaaga atgtgaaaaa gctaaagaaa tgttttatat aatgcaaaat
     1321 gatagtgtaa atgaaaaatt tgaaagattt tttaatactt atttaataca agaccacata
     1381 atggaaaata aaaaggttat gacagttggt tttcttgaag aaaatggtac aaacctaagt
     1441 caatcagaag tatcaatatt aaaaagtatg tttgaatcat acgtaagtat ttatgaggta
     1501 aaagaagttt ctgctggaaa aatagtatta aaggactgtt taagtggaaa tgaactttgt
     1561 acagaagatg taaaacttct gagaagtttt aaaatcggaa gttgtatgat agctagaata
     1621 gttgatattg aaggaactaa catattgata gatataacta taagtatatc aaatgaggtt
     1681 aaagatataa ttttaaagga tattatgaat ctattcaatc aatatcaaga tgtatacaaa
     1741 gatatgaagt cgttcttaat ttatcataca catatactat acaagtatat acaacagctt
     1801 ttagacccaa gtatagctga atatttaaag agagaaagag ataaaaacga tacaaaagtt
     1861 aattcagaaa atgaattagc tgaagaagaa tgtaaagttt tagatactct taaagaaaat
     1921 gtagaaaaag aaaatataga tgcatgtata gagttttgga atgaatataa gaaaaatcat
     1981 gaaaatataa aaggagctga aaatggttgg gcagctgctg ttgaatacta tgttaaaaaa
     2041 gaagcaggac aacctataac tcaagttcaa atatcaaaga aatacgatat aagctctagt
     2101 acactaggaa agagatacaa ggatttaaaa atatcttaat taattataaa aagctttaag
     2161 tcatagataa agaatctata gtataatata tgtataatgt ttagaaagag gtattaataa
     2221 tgagtcttag tttaggaaat gcaattgctg tatcaacgaa ctttataaaa tctaacccag
     2281 ctgtacctgt gtacaacgga gaagaaatat atgcagttat ttatcatata gaaataggat
     2341 ataataatgg tgtatcacct gataccaaac aatatataat aatagactat aaagagtttg
     2401 atttatctat ggataaaaaa aatgaactaa taaatattgc taaagagtac tgtaaatcta
     2461 aaggtattgt agaaaacgaa aaccttgaaa taaacctttt agacaatgaa gaagcagaga
     2521 acttcttaga aaaggtgttt aataatatga aggttataag aggtcttata actaaaaaag
     2581 gataaataaa tggactatat aggaatagaa aatataacac cttatgaaaa tacatatgaa
     2641 tttagtgtat atgaatatga tgatgaaatc accttaggta gtgaaaagtt atatgtatgt
     2701 gaattgaggg ttgtattgat taaagttaat tctctgtatg ttgaaagatt gcataaatca
     2761 gttgaagcaa tggtcttagt aaaaaacttg aaaaaagatt tagataaaac acttgttgta
     2821 aacaaaataa agaattttgt gctagatgag atttgggtag aaaatctagt aaaagagaat
     2881 atagaagtta tatttgtaga aagctaggaa taaccctagc ttttttatat tacaatctaa
     2941 gagaggaaaa caatatgtta agagtatcta atgtaaaact tggaattgat aaagatatat
     3001 ctttattaaa agatgttata cttaagaagc taaaaataaa agaaaaaaac ttagtcaaat
     3061 atagtatata taaagaatct atagatgcta gaaaaaaagg aaaaatggaa tttgtttatt
     3121 ctgtagatgt agaagttaaa gatgaaaata aaatacttaa ctcaaaaata aaagatgtaa
     3181 caaaaataaa ggaaatcaga tacgtaaatg tacctgtggg tgataaaaaa ttaaagaata
     3241 atcctttggt aattggtagt ggaccagctg gtttgtttgc aggtctgctt ttagctcaaa
     3301 tgggttatga accaatcata cttgaaagag gtcttgatgt agacagtaga accaaagata
     3361 taagtgattt ttggacaact agaaaattaa aaaataattc aaacgttcaa tttggagaag
     3421 gtggagcagg aactttttcg gatggtaaac ttacaactag aataaaagat atacgatgta
     3481 gaaaagtatt agaagagtta gttcagtttg gagcaccaga tgaaatatta tactcacata
     3541 agccacatgt tggaacagat atattaaaaa atgtagttaa aaatataaga aatgaaataa
     3601 taagtttagg tggtcaagtt agatttaatt caaaagtaac agatatagtt gtatcaaatg
     3661 attctataca atctgtaata ataaatgata ctgaaaaaat agatacagat acaattatat
     3721 tagctgttgg acatagtgca agagatactt atgaaatgtt gtattcaaga ggtataaaaa
     3781 tagttcaaaa accttttgca ataggagcta gagttgaaca tccacaagat ttaataaata
     3841 aagcacagta caaggatttt tataatcatc ctaggttagg agctgcagat tatagactta
     3901 tagagcatgt ttcaaatgga agaacagcat atactttttg tatgtgtcca ggaggtagtg
     3961 tcatagcatc tgcatcaaat gattttgaaa tagttacaaa tggtatgagt gaacatgcaa
     4021 gagataaaat aaatgcaaat agtgcatttt tagtaaatgt aacgccagaa gattttaaga
     4081 gtgataattc actagcaggt gttcatttcc agcagaagta tgaaaaatta gcttttgaat
     4141 taggtggaaa aaattataat gcaccagttc agcttgttgg agattttata aacaataaga
     4201 cttctttgaa tttaggaagt gttgagccat catatagacc aggatataca cttacaaatt
     4261 taagtgaatg tttgcctagc ttcgtaacag agactatgag agaagcgttt atatctcttg
     4321 ataaaaaatt gaatgggttt gcaatgtatg atgctgtttt gacgggagta gaaactaggt
     4381 catcatcacc aataagaata gtaagagatg aaaatacttt agaatcagtg agtattaaga
     4441 atctatatcc atgtggtgaa ggtgctggat atgcaggtgg tatagttaca gcagcagttg
     4501 atggtataaa atgtgcagaa aaaataatac aaaaatacac caataataca aaaatttaac
     4561 ataaacttaa tgatttttta atatttaaca tgaatttaac attatcactc ctataatttg
     4621 ataaaataca ataggttata aaatttttag atacagtaat ctctttacaa ggaggaaata
     4681 atttgaacat tataataagt ctaatgggtg gattaggtct gttcttatat ggtatgaacc
     4741 ttatgggaga aggtcttcaa aaatctgctg ggacaaagct aaaaaagata ataaaactac
     4801 ttactagtaa cctatttatg ggagttttgg taggtacagg agttacagca gtaatacaaa
     4861 gttcaagtgc tactactgta atggttgttg gtttcgtaaa tgcaggaata atgactttaa
     4921 aacaggcaat aggagttata atgggtgcta atatagggac tactgttaca gcccaattgg
     4981 tatctttcga cttaactggt atggccccag tagcattagg tgtaggtatt atactttatt
     5041 tatttggaaa taagcctaga ataaaaaata tagccgaaat acttataggt tttggtatat
     5101 tatttacagg tatggatttt atgaagatgg cagtggagcc actaagagac tatcaaggat
     5161 ttactgactt acttgttaca tttggtagat atccactact tggattatta ttgggatttg
     5221 ggattacagc aataatacaa agttcaagtg catctatggg tatgttggtt gcactggcag
     5281 cagaaggtct agtgccatta tcagcagcac tgccaattct ttatggccaa aatattggta
     5341 catgtgtaac ttcactgtta tcaagtattg gtgctaacaa aaatgcacga agagcagcta
     5401 tgatgcatct aatcttcaat gttttaggaa cagtgatatt cttaatattc ttgaataaac
     5461 cagtagtaag catggttaca agttgggacc caagcaatgt tgcaagacaa atagccaata
     5521 cacatacatt gtttaatata atatctgtat tgatactttt accatttaca aatcttatta
     5581 taaaactagc tataaagctt gttcctgata gagcaggtga tatagatgaa gatgaaacta
     5641 aaactattaa atatatagat gatagaatga tagaaactcc ttctatagct cttgcaaata
     5701 cgataaaaga agcacttcgt atgggtgaga aggcgaagga aagcctaaat gcatctatgg
     5761 aagctttagt agaacattca actgaaaaaa tagataagac atataggcga gagagattaa
     5821 tcaatgattt acagaaagct attttaaatt atttacttaa actttcaaaa gcacctttag
     5881 atgatgattc aagagaagtt gtggatactt tgtttaatac agtcaatgat atagaaagaa
     5941 ttggtgacca cgctaaaaat atagctgaat tatcgcaagt tgcaatagat tcaaatattt
     6001 ctttctcaga agaaggacaa tctgaacttg atgttatgta taatagagtt gtttcagcat
     6061 atacttatgc attagaatca atgagaactg ataatgttga tttagcttgt aaggttataa
     6121 aaattgaaga acaagttgat ataatggaga aatcttgtag agctaatcac atgtatagat
     6181 tgaacaataa tctatgtagt atagaaaatg gagttattta tttagacgta ataagtaatt
     6241 tagagagaat atctgaccat gctgtcaata tagcacaaca agttatagca aagagattag
     6301 gaaatgatta aaaataatta atttaaaata atataaaatt taaaatagag gataaataaa
     6361 agagaaactt taaatgatac gtttagagtt tctttttttg taaatttatt tgactaaaat
     6421 ttttaaattt atacatagtt atagcattta gaagaatcga tttcctgata ataaatgatg
     6481 agatatgtat gattttgtgg caaactttta aaaaaaacac ctatattttg tgttataatc
     6541 tctttaaaac gaatatgctg aaaagaggga tttaaatggg aaaaataaat tttgattata
     6601 gtaaagctac agagtttttt tgtcaaaacg aaatagatgt catgcaacca tatgtagatg
     6661 ttgcgcacga catgttacat aataaaactg gtttaggaaa tactttttta ggatggatag
     6721 accttcctaa aaattatgat aaagaagagt ttgacagaat aaaaaaatct gctgagaaga
     6781 taaaatcaga ctcagatgtt cttttggtaa taggtatagg aggctcttat ttaggttcga
     6841 gagctgctat agatatggta agtcattctt ttaggaatgg acttaaaaaa gagcaaagaa
     6901 aggctccaga agtatatttt gtagggcata atataagctc aacatatatt atggatttat
     6961 tagatataat tgaaggaaag gatatttctg taaatgtaat atctaaatca ggaactacta
     7021 cagaaccagc tctggctttt agaatattta aagattactt agagaaaaag tatggtaagg
     7081 aagaagcaag aaagagaatt tatgcaacaa cagatgcaag taaaggtgca cttagacagt
     7141 tagctacaga agaaggatat gagacttttg taataccaga tgatgtaggt ggtcgttttt
     7201 ctgtattgac agcagttggg ttacttccta ttgctgctgc tggacttgat attgatgcta
     7261 tgatgaaagg cgcaaatgat gctagagaag catttcaaaa tcctgactta aaaagtaatg
     7321 atagttatag atatgctgtt gctagaacaa ttttacatag aaaaggaaaa gatgtagagt
     7381 tattagtgaa ctatgaacct caactacatt atgtaagtga gtggtggaaa cagctttatg
     7441 gagaaagtga aggaaaagaa aataaaggtt tattccctgc ttctgtagat ttctcaacag
     7501 atttacattc tatgggtcaa tacatacaag atggaaaaag gcttttattt gagacagtac
     7561 ttaatgttga aaactgtaag agaaatataa ctataagttc agaggaagta gacttagatg
     7621 gtttaaacta tttagcaggt aagactgtag attttgtaaa tcataaagct tttgaaggaa
     7681 ctctattggc tcatacagat ggaaaagtac ctaatttagt aataaatata cctcaactag
     7741 atgagtataa ttttggatat ttagtttatt tctttgagaa ggcttgtgga ataagtggat
     7801 atttattggg agttaatcca tttgaccaac caggagtaga agcttacaag aaaaatatgt
     7861 ttgctttact aggcaaacca ggttatgaaa aagaaaagga agagctagaa aaaagactta
     7921 aataatttta aatagttaag gtttaattaa gattaaaata atataatgaa atcatcaaaa
     7981 aggagtttac tcaaaataga tgatttcatt ttttttatga aaaattggga aaaatatatt
     8041 ttgataattt atattatatt aagataggga gtggtatgag atgtcaagaa gaaagaaagg
     8101 gataagttta gtaatattag ttgctataat aagttcaata cttagtagtt ttctaacaat
     8161 aattttagtt aaggacaatt tagtaagcaa atctacagga agttctacgc cgatagttgt
     8221 aaatgatgat ggtaagagtc aaaatattta tcaagcagtt gcagaaaaag ctacgccatc
     8281 tgttgtaggt attacaacaa ctagtgttga tactagtaat atgtttgcaa taccaactga
     8341 aacacaaggt gtaggaactg gaatcatagt tgattcaaat ggatatatac ttactaattc
     8401 tcatgttata tcagatggtc aagctacgag tgtaaatgta ttattcaatg atggttcaac
     8461 aacttcaggt aaagtagtat ggtttgacca acaacttgat ttagctatag ttaaggttga
     8521 taaaactgga ctaacaccag cagaatttgc tgatagtgac aaagtaaaag ttggagatat
     8581 atcaatagca ataggtaatc ctcttggatt agattttcaa aaaacagtta ctcaagggat
     8641 aataagtgga ttagatagaa ctatacaaac agaaaaaact aatatgactg gtttattaca
     8701 aactgatgca agtataaatg caggaaatag tggaggacca ttgttaaatc aaaaaggtca
     8761 agtaattggt ataaacacag caaaagcttc tcaagcagaa ggattaggat ttgcaatacc
     8821 aatcaataca gctaagagta tagttgaaga agtaatcaaa aatggaaaat atgaaaaagt
     8881 aactttaggc ataaaaggta cagatgtatc aaattatgaa gcagcaacag gtacaaaatt
     8941 aagtactgat aaaggtgttt atgttgcaga agtaatatct ggttcatcag cagaaaaagc
     9001 tggagtaaag gtaggggata ttataactaa agtaggagat acagatataa caggtatgaa
     9061 tgatttaaat aaaaagttgt atactttctc aaaaggtgct agtacaaaaa taacagtaaa
     9121 tagaggcgga aaagcagtaa caataaatgt gaatttctaa atatgaccag aaagatagag
     9181 actacatggt aaagtgtagt ctctattttt atttaatttt tatagtatat acatctaaat
     9241 taattaaaat gatgtattgt ttatagctta ttgctatatt ttcttataaa aataaattta
     9301 ataaatattt aaatttaatt tacaaaaaca atataaatat cataataaaa taaaattttt
     9361 taatattact aaaaatatat agacaaaata ataacaaata tagtattatg taattgtaga
     9421 aatgcttttc ataggtatat tctatgaaga tgaattgcat aaaaatgaaa aaatggaatg
     9481 gatattattt aggggtgaaa aatcatgaat ccattagtta taaacttaca aaaatgtagc
     9541 atacatgatg gaccagggat aagaagtaca gtatttttta aagggtgtcc acttgaatgt
     9601 gtttggtgtc ataatccaga gagtcaaaca tatacaaaac aagttttgta taatgaagaa
     9661 agatgttcaa aatgtgaagc atgtataaat atatgccctc ataaggcaat ttacaaaggt
     9721 gaaacaaaaa tatgcttaga ccaagataag tgtgagtttt gtgaaacttg cttagactat
     9781 tgtgtaaaca atgcaagaga gattgttggt caagagtatt ctgtaagaga tttggttaaa
     9841 gagatagaga aggatagaat tttctatgaa gaatcaggtg gtggagtaac tctatcaggt
     9901 ggagaagtaa tggctcaaga tatggacttc atttgtggag taataaacat gtgcaaatca
     9961 aagggaatcc atgtagcaat tgatacatgt ggctatgcaa agtcagaaaa ttatgaaaga
    10021 gttgctaaat gtgcagattt gtttttatat gatattaagc ttattgatga agataagcac
    10081 ataaaattta caggaaaatc aaatgactta atactgaaaa atgttaagat tttaagtgaa
    10141 ttaggggcta acataaatat aagaatacca ttaattgtag gagtaaatgt agatgatgaa
    10201 aatttagagg ttaaaaaaat gatagagttt ttaaagcctt tgaatataca agcagtaagt
    10261 cttcttccat atcataatat tggtaaacac aagtatgata aaatttataa aaagtatgaa
    10321 ggagaagaac tacaaagacc atcagaagaa aaattagagg aaataaaaag actgtttgaa
    10381 gcaagtaatt tcaatactaa gattggaggg taataaaatg gcaagaggaa cttttgagag
    10441 aactaaaaaa ttaagagaag aaagtattaa tgcagaacca cacatatcta ttgaaagagc
    10501 agtacttatg acagaagctt ataaaaaata tgagggaagt gtagagattc ctgtacttcg
    10561 tgctttatca tttaagcatt atattgagaa tagaactctt agcataaatg atggagaatt
    10621 aatagtaggt gaaaaaggtg attctcctaa tggagcacca acatatccag aaatttgttg
    10681 tcatacaatg gaagatttag aagtaatgca caatagagat ataataaatt ttagcgtatc
    10741 agaagaagct agaaaaatac ataaagaaga aataattcct ttttggaaaa agagacaaac
    10801 aagagataag ataataaatg caatgacacc tgagtggtta gcagcatatg aagcaggtat
    10861 gtttacagag tttatggaac aaagagcccc aggacataca gtttgtggag atacaatata
    10921 taaaaaagga tttttagatt taaagaaaga catagaagca agattaaaag agctagattt
    10981 tcttaatgat ttagatgcat ataataaaaa agcagattta gaagctatgg caatagcttg
    11041 tgatgcaatg gttatattag gtaaaagata tgctgagaaa gcaagacaaa tggcagaaga
    11101 agaaacagat gaagctaaga aaaaagattt attactaatt gctgaaactt gtgatgttgt
    11161 accagctcac aaaccagaaa cttatcatca agctatacaa atgtattggt ttgttcatat
    11221 aggggttact actgaactta atatatggga tgcttttact ccaggaagac ttgaccaaca
    11281 tctaaatcct ttctatgaaa gagatgtaga aaatggaata ctagatagag atagagcaca
    11341 agaattactt gaatgtttat gggttaaatt taacaatcag ccagcaccac caaaagttgg
    11401 tataacatta aaagaaagta gcacatatac agattttgca aatataaaca ctggtggaat
    11461 aaatccagat ggtcaagatg gtgttaatga agttagttat ataatacttg atgttatgga
    11521 tgaaatgaaa ttgatacaac ctagttcaaa tgttcaaata agtaaaaaga ctcctcaaaa
    11581 attcttaaag agagcttgtg aaatatcaag aaaaggatgg ggtcaaccag cattttataa
    11641 tactgaagca atagttcaag agcttatgga agctggaaaa actatagaag atgcaagact
    11701 tggtggaacc agtggttgtg ttgaaactgg atgttttggt aaagaagcat atgttctaac
    11761 tggatatatg aacattccaa aaatacttga attaacatta aataatggat atgacccaat
    11821 tagtaaaaaa caaataggta ttgaaactgg agaccctaga aatttccaat cttatgagga
    11881 actatttgaa gcatttaaaa agcaattaca ttatatgatt gacataaaaa tagaaggaaa
    11941 tgctgtgata gaaaatatat gtgcaaaaca catgccttgt ccacttatgt ctacaatagt
    12001 tgatgattgt atagaaaaag gcaaggatta ccaaagaggt ggagcaagat acaacactag
    12061 atatatacaa ggtgttggta taggaactat aacagatagt ttaacagcaa taaaatataa
    12121 tgtatttgat aagaaaaaat tcgatatgga tacattgctt aaagcacttg atgctaattt
    12181 tgaaggatat gaagctatac ttaatttagt ttcaaataag actcctaaat atggaaatga
    12241 tgatgattat gctgatgaaa taatgcaaga gatattcaat gcatattaca atgaggtaac
    12301 tggtagacca actgtttgtg gtggagaata cagagtagat atgttaccaa caacttgtca
    12361 tatatacttt ggagaaatta tgggagcaag tccaaatggt agactttgtg caaaaccagt
    12421 ttcagaggga atttcacctg aaaaaggtgg agatactaat ggacctacag cagttataaa
    12481 atcttgtgct aagatggacc acataaaaac tggtggaaca ttattaaatc aaagatttgc
    12541 accatcagta gttcaaggag aaaaagggtt agataatatg gctaatctag taagagcata
    12601 ctttaatatg gatggtcatc atatacaatt taatgtattt gataaaaatg tactgttaga
    12661 ggctcaaaag aatcctcagg attataaaga cttaatagtt agagttgcag gatatagtga
    12721 ccatttcaat aatttaagta gaacactaca agatgagata ataggaagaa ctgagcaaac
    12781 attctaggtt tgcttgtaaa taaaacttct tactatataa aatcgtaact gatataataa
    12841 agtataaatg gtttgattcc atttttaaaa ttttagatta attatgagct aactttctaa
    12901 ataggaagtt agctcgttaa gtttaatgaa aaaatcgtta aatttatatt tagtaaatca
    12961 tacacatatt agtaagtaat acatacaaaa gaataattta ttatagttat aataaggtta
    13021 tattttataa aaataagata attttaagca gttaaaataa agtcaattta gctactttaa
    13081 aataaaacta atagaaaaaa ggagatttga tatgaaaact ttaggattta ttggttcagg
    13141 gaatatggga agtgcaatga taggtggcat agtacagtca gaattagtaa aggcagaaga
    13201 tataactgta tcagatttaa gccaagttgc cttagataaa gtaaaagaag aatttggagt
    13261 aaatactaca actgattcaa aggtagctgt tacaaattct gatgtggtaa tagtagcact
    13321 taaacctaat atatgcgaaa aggctttaac tccacttaaa gaattaatag atgcaaataa
    13381 aataatagta tctatagcag caggtaaaac tatagaaagt ctggaaagtt ttataggttc
    13441 agataaaaaa atagtaagag taatgcctaa tactccagcc cttgttggcg aaggaatgtc
    13501 agctctttgt aaaaatagta atgtaacaga tgaagagtta aatatgatta aggctctgtt
    13561 tgaatctttt ggtgaggcag aaatagtatc agaatattta atggatacag ttacaggtat
    13621 tagtggttct tctcctgcat atgtatttat gtttatagaa gctatggcag atgcagcagt
    13681 attagctgga atgccaagac aacaagctta taaatttgca tctcaagcag taatggggtc
    13741 agctaagatg gttcttgaaa ctggaaaaca tccaggtgaa ttaaaagata tggtttgttc
    13801 tccagcagga actactatag aagctgttaa ggtattagaa gaagaaggct ttagagcagc
    13861 agttataaaa tctattgttg cttgtataga taagtctaaa gatatgagca aataacagat
    13921 gtaattaagt ttactatatg gtttcttgaa ttgttttttt agtgcttatt tatatctata
    13981 cataaatgta tttggtataa gtaaaataag agtaatttac tataaaaagg aatttaatga
    14041 ttgagtgaga atttattact t
//
LOCUS       DAELLV010000087        13999 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.087, whole genome shotgun sequence.
ACCESSION   DAELLV010000087 DAELLV010000000
VERSION     DAELLV010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13999
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.087"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            444..1514
                     /locus_tag="KPD58_002013"
     CDS             444..1514
                     /locus_tag="KPD58_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0265779.1"
                     /translation="MADIRLENVTKSYNKKNTVIENLNLTIEDGSFTVLVGPSGCGKS
                     TTLRMIAGLEDITSGRLKIEENDVTKTDASKRDIAMVFQNYALYPHMTVRENIEFGLK
                     NKKVEKEKREKLITEVIDVVGLREYLNVKPGNLSGGQRQRVALARAMVKNPKVFLMDE
                     PLSNLDAKLRNQMRTELIQLHQKLKSTFVYVTHDQVEAMSMADKIVIMNKGKIMQIGS
                     PKKIYQNPKNLFVAQFIGSPCMNILDIKNDIKFGFRPEKATLESINIDGKNDDVFSLF
                     GKVVTKEVLGNETIYCLSVLNREIRVKTENDIFDIGRTLRISVLEKDLYYFDKNGERI
                     EDVKVCKSMYSKIKGDIYEKAI"
     gene            1498..2367
                     /locus_tag="KPD58_002014"
     CDS             1498..2367
                     /locus_tag="KPD58_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBG0265780.1"
                     /translation="MKKQFNKAYLYITPAIIGTLLFIVYPIIKTISLSFKSGSLLSST
                     FENVGLNNYTELLKNPEFIQTITNTAIYTFFMVTISISLAIVLAVWLNKNSIIHNLTQ
                     SIIFTPHVISLVSVAVLWLWIMEPQYGFLNWILSTLHLPTSKWLSSESSALMSLILIG
                     IWKTLGYNILIVLSGLKSIPNYIYEASKLDNANKITNFFKVTLPLLSPTIFFLMITTT
                     TASFQVFDEISIMTQGGPVGSTNMLSYYIYESGFIYYDIGQASAASMFLLAIVMIATY
                     ANFRLLSKKVHYQ"
     gene            2411..3271
                     /locus_tag="KPD58_002015"
     CDS             2411..3271
                     /locus_tag="KPD58_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBG0265781.1"
                     /translation="MDIIQPIKKDKVDIKKIASKSIEILILLLLSAIFIFPFIWMLST
                     SFKNPQEMMQLPPTIIPNDIVLENYSTAWHSGPFFRYLLNSVFVTGSVLIVQFLVMVP
                     AAYAFSKIKFKGEKMLFGLLLVGLMIPPQVTFLPVYMMMSKFGFINTYVPLIIPFMTS
                     SFGIFLLRQNFMQISDEIIEAAKLDNASDLKIMFRIMVPMAKPAVITFILFNFIYHWN
                     NYFWPLVMTNTDAIRTLPIGVALLKSSEGITAWNVIMAGNIILILPIILVYIFANKKV
                     KEAFMYSGIK"
     gene            3302..4606
                     /locus_tag="KPD58_002016"
     CDS             3302..4606
                     /locus_tag="KPD58_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0265782.1"
                     /translation="MKLKRKLATGLAITLLLGSLVGCSNSNDANASKGEKTDSNITKI
                     DFWAPLGGTNGETAQKMVDQFNSEHKDVQVNMLKQKDYYENATKLQAALTSKDQPDVT
                     LLEITQTGTFASAGALVDMSKYFDKTYQERFFSGLLTNSYYEDKFVGMPFNRSTPILY
                     INKDMATKAGLDPSGPKSWEELKDYASKMTNKSEDTYGFETPIDIWFYEAMVMQSGGE
                     ITNGKKVAFNNEAGQAPVKFWQDMMKAGIMKMPPGEDYNAWDVAKQDFVNGKVGMIFT
                     STADLAGLMQQTEGKFEISTAFLPKNQKYATPTGGANLVMLEGGTDKEKEASAEFIEW
                     MTQTDKIVQFSSSTGYLPTTEDATNSEKLQTLYKEKPQYKVATDQLQYAVALPMLNGY
                     KEATDKLMDEIKKGLTDLNANPKDVVSKGTSAMQAVIDKTNK"
     gene            4669..5931
                     /locus_tag="KPD58_002017"
     CDS             4669..5931
                     /locus_tag="KPD58_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HBG0265783.1"
                     /translation="MTKVIVLSVDSLFEKDLEFVKNLPNFKSILENCSIVKNINCVYP
                     TLTYPCHTSMVTGVYPKKHGIYHNEKFDPNKENKDWYWYSKDIKAKTIIDVAKENNLT
                     TSSVLWPVMGANPNIDYNIAEIWAPSREDDPRETFERSSSKVIMDGIYNRHCHYIDWK
                     LEPNMDLFGVNCSVDIIKEYKPDLMLIHLATLDHTRHNYGLFNDEVDKALKMNDKWLG
                     DIIQATKDAGTYKDTNFIILGDHGHLRVDKVINPNVLLKSNGFIKVENGEVKDFDAYV
                     NSSGISAQIIVNNLDRLTELRELLEEFKEELFIENIFTKEEASNLGLEGDFEIVIEGL
                     EGISFGNDFEGSIIKDSDIKDYKFAVATHGHLPTKGNRPPFIAFGPNIKGGVVIEEGD
                     LRAHAATILKMFNLKLDGVEKEAFDFIM"
     gene            6118..8088
                     /gene="uvrB"
                     /locus_tag="KPD58_002018"
     CDS             6118..8088
                     /gene="uvrB"
                     /locus_tag="KPD58_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HBG0265784.1"
                     /translation="MDFKIKSDFKPTGDQPEAIKSIVDSINRNEKFSTLLGVTGSGKT
                     FTMANIIQQVKKPTLIMAHNKTLAAQLYSEFKEFFPDNAVEYFVSYYDYYQPEAYVAH
                     SDTYIEKDASINDEIDKLRHSATASILERRDTIIISSVSCIYGLGDPKDYKELMLSIR
                     RGMQRDRDDVIKRLIEIQYERNDINFTRGTFRVRGDILEIFPASNDEKAIRIEFFGDE
                     VDRITEIDYVTGKIVGTRNHVVIFPASHYVTTPERIEKAIVEIENELQEQIKFFKEND
                     RLLEAQRIEQRTKYDIEMLKEIGFCQGIENYSRHITGRSEGERPYTLMDFFPDDYLII
                     IDEAHVTIPQVRGMYAGDRSRKTSLIENGFRLPSALDNRPLNFQEFEGNINQMLFVSA
                     TPGPYEIQHSETIAEQIIRPTGLLDPIVEVRPINNQIDDLVGEITKTIEKNERVLITT
                     LTKKMSEDLTNYLKEIGIKVKYLHSDIVTLERTEIIRDLRLGKFDVLVGINLLREGLD
                     IPEVSLIAILDADKEGFLRSETALIQTIGRAARNENGRVIMYADRITDSMQNAIDETK
                     RRRDIQNLYNEEHNIIPKTIQKNIRDSIEATKVAEEEVVYGISDTDDKDEIRANIDKL
                     KSEMMEAAQNLQFERAAELRDKVKQLEEKLEK"
     gene            8107..10932
                     /gene="uvrA"
                     /locus_tag="KPD58_002019"
     CDS             8107..10932
                     /gene="uvrA"
                     /locus_tag="KPD58_002019"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG0265785.1"
                     /translation="MEDKIIIRGAKEHNLKNIDLELPRDKFIVFTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMEKPNVEYIEGLSPAISIDQKTTSKNPRSTVGTV
                     TEIYDYLRLLFARVGDVYCPTCGEKISQMTIQEIVDKMLDFPDRTKLQILSPIVRGQK
                     GTHKKVFDNIKKEGFVRVRVNGENYEVSDDIILNKNQKHNIEVVVDRIVIKDGIESRL
                     ADSIETAVKLSDGLVIAQIIDGEEILFSTKFACPEHGIGIEELSPRMFSFNAPFGACD
                     VCNGLGESKEVDPELVVPNKDLSIKQGAVAAWGSTGVNDDTYYSKMIKSLAEHFNVSL
                     TTPFKDLPDDFVEELLYGKDNIIVEFTYESKFGGTRNYKSYFEGVIVNLERRYRETNS
                     DYMRDKIEEYMAERPCPKCKGMRLKKEVLSVLVDGKNIMEVTNLSVNELIDFMENINL
                     TEKQRFIAHEIIKEIKGRAMFLRDVGLDYLNLSRKAGTLSGGEAQRIRLATQIGSALV
                     GVLYVLDEPSIGLHQRDNDRLITTLRHLTDLGNTLIVVEHDEDTMREADYVIDIGPGA
                     GIHGGEIVAQGTLDEIIENENSITGQYLSGKKQILLPETTREGNGSFIEIVKASENNL
                     KNIDVKFPLGKFTCITGVSGSGKSTLINDILYKGVASKVNRLKQRAGKHKEIKGIESI
                     DKVINIDQSPIGRTPRSNPATYTGVFDFIRDIFASTNEAKARGYKKGRFSFNVKGGRC
                     EACKGDGIIKIEMHFLPDVYVPCEICKGERYNRETLQVKYKDKTISDILDMNVEEAVE
                     FFENIPNIKRKLETLMDVGLSYIKLGQPSTQLSGGEAQRIKLAAELSKRPTGKTLYIL
                     DEPTTGLHMADVDKLIQVLQRLADTGNTIIVIEHNLDVIKTCDYVIDLGPEGGDKGGT
                     IVATGTPKEVSKVDGSYTGQFLKKYFS"
     gene            11191..13008
                     /gene="uvrC"
                     /locus_tag="KPD58_002020"
     CDS             11191..13008
                     /gene="uvrC"
                     /locus_tag="KPD58_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBG0265786.1"
                     /translation="MFDIQEHLKKLPSEPGVYLMKDKYDHIIYVGKAISLKNRVRQYF
                     QSSKNHTSKVKSMVKNIYKFEYIITDSELEALILECNLIKRYRPKYNVVLRDDKTYPY
                     IKVTTNEDYPRILKVRRVLKDKAKYFGPYTNITAVNDTLELISSTYPIRSCKIDIDKA
                     IKNKTRPCLNLHINKCLGPCTGNVSKEEYGKMIEEIIMCLSGKEEKLMELLKEKMNES
                     SMNFRFEEAAVYRDKIKSLEEMIQKQKIDATVSDLNQDVVAMARAHNEACVQVFFIRN
                     GKIVGREHFILEGVMDSPRASILSSFVKQFYNEQEYIPKELIIEDEIEDSSILEEWLS
                     SKKGQKVTIRVPQKGEKKSLVEMVRKNAVEYLEKFSDMNKRKYEKSIGALEELKQILN
                     LEKLPIRIEAYDISNIQGVDSIGSMVVYTNAKKDKKEYRRYKIKTVIGPNDYDSMAEI
                     VDRRLKHGNLPDLILLDGGKGQVSAVKKVLELNDVDIPLWGMYKDDKHRTKGLICKEK
                     EIELDKTTNLYRFIASIQEEVHNYAITYHRSLRNKALTKSILDDIQGIGEKRKKSLLN
                     HFKDVDAIKKATMEELLEVDGMNKSIADNVYNFFRKEEN"
     gene            13008..13937
                     /locus_tag="KPD58_002021"
     CDS             13008..13937
                     /locus_tag="KPD58_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HBG0265787.1"
                     /translation="METSNKVSIREIIKELKLKVVYMPDDVDYYVYSQDINRPGLQFA
                     GYFDYFAYERIQIVGKTEYNFFGLMDSHIREEVLDRFFSYEIPALIISRDLEIKPDVI
                     EKAKKYKRILLSTNRNTTRLINRLSNYLDNKLAPHTTIHGVLVDIYGIGVLIKGESSI
                     GKSETALELIQRGNRLVADDAVEIRKLDESMLVGQSPELIRHFLEIRGIGIIDVRSLY
                     GVGAIKNSKMIDLVVHLEAWDETKYYDRLGLDKEYEEILGVNVEKLVVPVKPGRNTAM
                     ILEVAAMNFRQRGMGYDAAQEFTKKLSKLIDTK"
BASE COUNT         5695 a         1610 c         2545 g         4149 t
ORIGIN      
        1 ggtaaattta taaaaattaa aaaagtttta ttttttatgt ttaaaccttg taaatttggg
       61 acataatata attgtaaaac ttaatatagc aaaaataaat tactcaatct cccccactta
      121 aagacctcta tcccccatag aggtcttttt tattttcaaa ccaaattata taaaaaataa
      181 atagttttat atgactcttc ataaattaaa atcacattgt gatagcaaac catgaatatg
      241 aaaaatatag aaaattatag ctaacataag ctgtatatca tcttaacttc agtatattta
      301 acaaaaaatg tttataattt aacctttatt ttaataagtt tactatatat tcactttgtc
      361 ttaatatatc ctttacaaat taagagtatt attaatctat aagtaaatcg tagaattaaa
      421 ttaaatattt tgggaggata aaaatggcag atataagact agaaaatgta acaaaatctt
      481 ataataaaaa aaatacagtt atagaaaatt taaatcttac tatagaagat ggttctttta
      541 cagtattggt aggcccaagt ggatgtggta aatctactac gttaagaatg attgcaggtc
      601 ttgaagatat aacttcaggt aggttgaaga ttgaagaaaa tgatgttaca aaaacggatg
      661 ctagtaaaag ggatatagct atggtatttc aaaattatgc attatatcca catatgactg
      721 ttagagaaaa catagaattt ggtcttaaaa acaaaaaagt agaaaaagaa aaaagagaaa
      781 aacttataac agaagttatt gatgtagtag gattgagaga atatttaaat gtgaaaccag
      841 gcaacttatc tggaggtcaa agacaaagag tggcacttgc tagagctatg gttaaaaatc
      901 caaaggtatt tttaatggat gaaccattgt caaatttaga tgctaaatta agaaatcaaa
      961 tgagaacaga attaatacaa ttacatcaaa aactaaagtc tacatttgtt tatgtaactc
     1021 atgaccaggt tgaggcaatg tcgatggcag ataagatagt tataatgaac aaaggaaaga
     1081 taatgcaaat aggttctcca aagaagatat atcaaaatcc taaaaattta tttgtagctc
     1141 aatttatagg gagtccttgt atgaacatac tagatataaa aaatgatatt aaatttggat
     1201 tcagaccaga aaaagcaaca ctagagagta taaacataga tgggaaaaat gatgatgtat
     1261 ttagtctatt tggaaaagtt gtaactaaag aagtgttggg aaatgaaact atatattgtt
     1321 tatctgttct taatagagag ataagagtca aaacggaaaa tgatatattt gatattggaa
     1381 gaactttaag aataagtgta ttagaaaaag atttatatta ttttgataaa aatggagaaa
     1441 gaatagaaga tgtaaaagta tgtaaaagta tgtattctaa aattaaagga gatatttatg
     1501 aaaaagcaat ttaataaagc atatttatat ataacacctg ctattatagg aactttattg
     1561 tttatagtct atccaataat taagacgata tctctaagct tcaaatctgg tagtcttctt
     1621 agttcaacct ttgaaaatgt aggcttaaac aattatacag aacttttaaa gaatccagag
     1681 tttatacaaa ctataacgaa cactgcaatt tatacttttt ttatggtgac tatatctata
     1741 tcactagcca tagtacttgc tgtttggcta aataaaaatt ctataataca caatttaacg
     1801 caaagtataa tctttacacc acatgtaatt tcactagtat ctgtagctgt attgtggctt
     1861 tggattatgg agccacaata tggcttttta aactggatac tatcgacatt acatttacca
     1921 acatcaaagt ggctttcaag tgaaagttca gctttaatgt cactaattct aataggaata
     1981 tggaagactc ttggatataa tatcttaata gtgttatcag gtcttaaatc cataccaaac
     2041 tatatatatg aagcatctaa attagataat gcaaacaaaa taacgaattt ctttaaagtg
     2101 acattgccac tattatcacc aacaatattt tttcttatga taacaacaac aactgcatct
     2161 tttcaagtat ttgatgagat aagtataatg acacaaggtg ggccagtggg ttcaactaac
     2221 atgttatctt attatatata tgagtctgga tttatatact atgacatagg acaagcgagt
     2281 gcagcgagta tgtttttgtt agctatagtt atgattgcta catatgctaa ctttagactc
     2341 ttatcaaaga aagttcatta tcaataaaga aaaagtagat attataaggt ttatccaaaa
     2401 ggaggagaca atggatatta tacaacctat caaaaaagac aaagttgata taaaaaaaat
     2461 agcatcaaaa tcaatagaaa tattaattct attactttta tctgcaatat ttatattccc
     2521 ttttatatgg atgctatcta catcctttaa aaatccacaa gaaatgatgc aattaccgcc
     2581 cactattata ccaaatgata tagtgttaga aaattatagt acagcatggc attcaggtcc
     2641 attttttaga tatttgctaa acagtgtatt tgtaacagga agtgttttaa ttgtacaatt
     2701 tttagttatg gttccagcag catatgcatt ttctaagatt aaatttaaag gtgaaaaaat
     2761 gctatttggg cttttgcttg taggacttat gattccacca caagtaactt ttctaccagt
     2821 ctatatgatg atgagtaagt ttggatttat aaatacttat gttccactta taattccatt
     2881 tatgacatct tcatttggga tatttctgct tagacagaat tttatgcaga tttcagatga
     2941 aattattgaa gcagctaaac tagataatgc ttctgactta aaaataatgt ttagaataat
     3001 ggttcctatg gcaaaaccag cagtaataac atttatatta tttaatttta tataccattg
     3061 gaataattat ttctggccct tagtaatgac aaatacagat gcaataagaa ctttaccaat
     3121 aggagttgcg ctccttaaat cttcagaagg aattactgca tggaatgtaa taatggcagg
     3181 aaatataatt ctaattttac caataattct tgtttatata tttgcgaata aaaaagtaaa
     3241 agaagctttt atgtattcag gaataaaata aaaaattaaa gactaaattt ggaggatgaa
     3301 aatgaagctt aaaagaaaat tagcaacagg attagcaata acattattat taggttcttt
     3361 agttgggtgt tcaaattcta atgatgcaaa tgcatctaaa ggagaaaaga ctgatagcaa
     3421 tattacgaag atagatttct gggctccttt aggtggaact aatggagaaa ctgctcaaaa
     3481 aatggttgac caatttaata gtgaacataa ggatgtacaa gtaaatatgt taaaacagaa
     3541 agattactat gaaaatgcaa caaaactaca ggcagcctta acatctaaag accaaccaga
     3601 tgtaacatta ttagaaataa ctcaaacagg tacattcgca tcagcaggtg ctttagtaga
     3661 tatgagtaaa tattttgata aaacatatca agaaagattc ttttctggtc ttttaacaaa
     3721 ttcatattat gaggataagt ttgttggtat gccatttaat agaagtacac caatattata
     3781 cataaataaa gatatggcaa ctaaagcagg tcttgaccct tcaggtccaa aatcatggga
     3841 agagcttaag gattatgcta gtaaaatgac aaataaatca gaagatactt atggttttga
     3901 aacgccaata gatatttggt tctatgaagc tatggtaatg caaagtggag gagaaataac
     3961 taatggtaaa aaagtagcat ttaataatga ggcaggacaa gcaccagtta agttttggca
     4021 agatatgatg aaagctggaa taatgaaaat gcctcctgga gaagattata atgcctggga
     4081 cgtagcaaaa caagattttg taaatggtaa agtaggtatg atatttacat caacagcaga
     4141 tttagcagga cttatgcaac aaacagaagg taagtttgaa atatcaacag cattcttacc
     4201 aaaaaatcaa aaatacgcaa ctcctacagg tggagctaat ctagttatgc ttgaaggtgg
     4261 aacagataaa gaaaaagaag caagtgcaga atttatagaa tggatgacac aaacagataa
     4321 aatcgttcag tttagttcat caacaggata cttaccaaca actgaagatg caacaaactc
     4381 agaaaagtta caaacattat ataaagaaaa acctcaatat aaggttgcaa ctgaccaatt
     4441 acaatatgct gttgcacttc caatgttaaa tggatacaag gaagcaactg ataagctaat
     4501 ggatgagatt aaaaaggggc ttactgattt aaatgcaaat ccaaaagatg tagtaagcaa
     4561 aggaacttct gcaatgcaag cagtaataga taaaacaaat aaataattat atagacttgt
     4621 aagttgatta aaagtattca ataatatata aatgaagagg taatgtaaat gacaaaggtt
     4681 atagtattat cagtagattc tttatttgaa aaagacttgg aatttgtaaa aaatctacca
     4741 aactttaaat ctatactaga aaattgctcc atagtaaaaa atataaattg tgtttatccc
     4801 actctgacat atccgtgtca tactagtatg gttacaggag tatatcctaa aaaacatggt
     4861 atatatcata atgaaaaatt tgacccaaat aaagaaaata aagactggta ctggtactca
     4921 aaggacataa aagcaaaaac tattatagat gtagcaaaag aaaataatct aactacctct
     4981 agtgtattat ggcctgtaat gggagcaaat ccaaatatag attataatat tgcagaaata
     5041 tgggctccta gtagagaaga tgacccaaga gaaacttttg aacgaagttc cagtaaggtt
     5101 ataatggacg gtatttacaa tagacattgt cattatatcg attggaaatt agaacccaac
     5161 atggatttat ttggagtaaa ctgttcagta gatataataa aggaatataa accagattta
     5221 atgctaatac atttagctac attggaccat acacgacata attatggatt atttaatgac
     5281 gaagtagata aagcattaaa aatgaatgat aaatggctag gagatattat tcaggctaca
     5341 aaggatgcag gtacatacaa agatacaaat tttataatac ttggggacca tggtcatctt
     5401 agagttgata aagttataaa tccaaatgtc ttgcttaaat caaatggatt tataaaagtt
     5461 gaaaatggag aagttaagga ttttgatgcc tatgtaaatt cttctggaat atcagctcaa
     5521 ataatagtaa ataacttaga taggttaaca gaattaagag aactgcttga agaatttaaa
     5581 gaagaattat tcatagagaa tatttttacc aaagaggaag catctaacct tggtcttgaa
     5641 ggtgattttg aaatagtgat tgaaggtctt gaaggaattt catttggaaa tgatttcgaa
     5701 ggaagtatta taaaagattc agatataaaa gattacaagt ttgctgttgc gacacatggt
     5761 catttaccta ctaaaggtaa tagacctcca tttatagctt ttggaccaaa tattaaaggt
     5821 ggtgtggtta tagaagaagg agacctgaga gcacatgcag caactatatt aaaaatgttt
     5881 aatttaaaat tagatggagt tgaaaaagaa gcatttgatt ttattatgta agttgaataa
     5941 agtatttatt ttccatgaat tatagacttc tattgcaaag atagaagtct ttttttatgt
     6001 tttagattta agtaaaattt aaatgggtaa aatatttaaa ataacttaaa caattttata
     6061 aaggtatgat ataataatac atgtcaggaa ctaatgttcg aaaaaaggag tgatactatg
     6121 gattttaaaa taaaatcaga cttcaaacct acaggtgacc aaccagaagc tattaaatca
     6181 atagtggatt ctataaatag aaatgagaaa ttttcgacat tattaggtgt aacgggttca
     6241 ggaaaaacat tcacaatggc aaatatcatt caacaagtta agaaacctac cctaataatg
     6301 gctcataata aaactctagc agcacaactt tacagtgagt ttaaagaatt tttcccagac
     6361 aatgcagttg aatactttgt aagttactat gattattatc aaccagaagc atatgtagct
     6421 cacagtgaca cttatataga aaaagatgca agcataaatg atgaaataga taaacttcga
     6481 cattctgcta cagcatctat tttagagaga agagatacta taataatatc atctgtatct
     6541 tgtatatatg gacttggtga cccaaaagac tataaagaac ttatgttatc tataagacga
     6601 ggtatgcaaa gagacagaga tgatgtaata aaaaggttaa tagagataca atacgaaaga
     6661 aatgacataa actttacacg aggtaccttt agagttagag gagatatact agaaatattc
     6721 ccagctagta atgatgaaaa agcaataaga atagaatttt ttggtgatga ggtagataga
     6781 ataacagaaa ttgattatgt aacagggaag attgttggta ctagaaatca tgttgttata
     6841 ttcccagcat ctcactatgt aacaacgcca gaaagaattg aaaaggcaat tgtagaaata
     6901 gaaaacgagc ttcaagaaca aattaaattt ttcaaggaaa atgataggct attagaagca
     6961 caaagaattg aacaaagaac taagtatgat atagaaatgt taaaggaaat aggattttgt
     7021 caggggatag agaactattc tagacatatt acaggaaggt cagaaggaga aagaccatat
     7081 actttaatgg atttcttccc agatgattat ttgataatta tagatgaggc acatgtcact
     7141 ataccacaag taagaggtat gtatgcagga gacaggtcta gaaaaacatc acttatagag
     7201 aatggtttta gattaccttc tgcacttgac aatagaccat taaattttca agaatttgaa
     7261 ggaaatataa atcaaatgtt gtttgtaagt gctacaccag gaccttatga aattcaacac
     7321 tcagagacaa tagcagaaca gataataaga ccaactggcc ttttagaccc aattgtagaa
     7381 gtaagaccta taaataatca aatagatgat ttggtaggag aaataactaa aactatagaa
     7441 aaaaatgaaa gagtgcttat aaccacttta actaaaaaaa tgagtgagga cttgactaac
     7501 tatcttaaag aaatagggat aaaagttaaa tatttacact cagatatagt tacattagaa
     7561 agaacagaaa taataagaga tttaagatta gggaagtttg atgtccttgt aggaataaac
     7621 ttacttagag aaggattgga tataccagaa gtttcactta tagcaatttt agatgcagac
     7681 aaagaaggtt tcttacgttc cgaaacagct ttaatacaga ctattggacg tgcagctaga
     7741 aatgaaaatg gaagagtaat tatgtatgca gacaggataa ctgactcaat gcaaaatgct
     7801 attgatgaaa caaagagaag aagagatata caaaatttat ataatgaaga gcacaatatc
     7861 attcctaaaa ctatacagaa aaatataaga gatagtatag aagctactaa ggttgcagag
     7921 gaagaagttg tatatggaat atctgataca gatgataaag acgaaataag agctaatata
     7981 gataaattaa aatcagagat gatggaagct gctcaaaact tacaatttga aagagcagca
     8041 gaacttagag ataaagtgaa acaattagaa gaaaaattgg aaaaatagaa aggtaaaaac
     8101 aacattatgg aagataaaat tataataaga ggagcaaaag aacataattt aaaaaatata
     8161 gatttagaat taccaagaga taaatttatt gtttttacag gtttgtcagg gtctgggaag
     8221 tcttctttgg catttgatac aatatatgca gaaggacaga gaagatatgt agaaagtcta
     8281 tctgcttatg caagacaatt tttaggtcag atggaaaagc caaatgtaga atatatagag
     8341 ggtttatcac cagctatctc aattgaccag aagactacct caaaaaaccc acgttctacg
     8401 gttggaacag taacagaaat atatgattat ttgagactat tatttgcaag agtaggagat
     8461 gtgtattgcc ctacatgtgg tgaaaaaata agtcagatga ctattcaaga aatagttgat
     8521 aaaatgctag attttccaga tagaacgaaa ttacaaattc tttcaccaat tgttagagga
     8581 caaaaaggta cacataaaaa agtctttgat aatatcaaaa aggaaggatt tgtaagagtt
     8641 agagttaatg gtgaaaacta tgaagttagt gatgatataa tcttaaataa aaatcaaaaa
     8701 cataatatag aagttgtagt ggacagaata gtaattaaag atggaataga atcaagacta
     8761 gcagattcaa ttgaaactgc agttaagcta tctgatggtc ttgtaatagc ccaaataata
     8821 gatggagaag aaattctttt ttcaactaaa tttgcatgcc cagaacatgg aattggcata
     8881 gaagaattat ctccaagaat gttttcattt aatgctccct ttggtgcttg tgatgtttgt
     8941 aatggtcttg gggaaagtaa agaagttgac ccagaattag tagtaccaaa caaagattta
     9001 tcaataaaac aaggtgcagt ggcagcatgg ggttcaacag gtgttaatga tgacacttat
     9061 tacagcaaaa tgataaaaag tttagcagag cattttaatg tatctttgac aacaccgttt
     9121 aaagatttac cagatgattt tgtggaggaa ttactttatg gtaaagataa tataattgta
     9181 gagtttactt atgagtcaaa atttggagga actagaaatt ataaatcata ctttgaaggt
     9241 gtaatagtaa acttagagag aagatataga gaaactaatt cagattacat gagagataag
     9301 attgaagaat acatggcaga gagaccatgt ccaaagtgta aagggatgag acttaaaaag
     9361 gaagttttat ctgttttagt tgatggaaaa aatataatgg aagttacaaa cttatctgta
     9421 aatgaattga ttgactttat ggaaaatata aatcttacag aaaaacagag atttatagct
     9481 catgagatta taaaagagat aaaaggaaga gctatgttct taagagatgt aggtcttgat
     9541 tatttaaatt tatcaagaaa agctggaact ttatcaggag gagaagcgca acgtataaga
     9601 ttagctactc aaataggttc tgcattggtt ggagttttat atgtacttga tgagccaagt
     9661 atagggcttc accaaagaga taatgatagg cttataacta ctcttagaca tctaacagat
     9721 ttaggaaata ccttaatcgt tgtagagcat gatgaagaca caatgagaga agctgattat
     9781 gttatagaca ttggtccagg tgcaggtata catggtggtg aaatagttgc acagggtact
     9841 ttagatgaaa taattgaaaa tgagaactca ataacgggtc aatatttaag tggtaaaaaa
     9901 caaatattgc ttcctgagac tactagagaa ggaaatggaa gttttataga aatagttaaa
     9961 gcatcagaaa ataatctgaa aaatattgat gtaaagttcc cattaggtaa gtttacatgt
    10021 ataacaggtg tttcaggttc cggaaaaagt acattaataa acgatatact ttacaaaggt
    10081 gttgcaagta aagtgaatag acttaagcaa agagctggta agcataagga gataaaagga
    10141 attgaaagta tagataaagt tataaatata gaccaaagtc ctattggaag aacacctcgt
    10201 tcaaatcctg ctacttatac aggtgtattt gattttataa gagatatttt tgcatctact
    10261 aatgaagcta aagcaagagg ttataaaaaa ggtagattta gctttaatgt aaaaggtggt
    10321 agatgtgaag cttgtaaggg tgatggaata ataaaaatag aaatgcattt cttaccagat
    10381 gtatatgttc cttgtgaaat ttgtaaaggt gaaagataca atagagaaac attgcaagta
    10441 aaatacaaag ataaaactat atctgatata ttagatatga atgtggaaga agctgttgag
    10501 tttttcgaaa atataccaaa tataaaaaga aaattagaaa ctttaatgga tgtaggactt
    10561 tcatatatta aattaggaca gccttcaact caattatcag gaggagaagc acaacgtata
    10621 aaattagctg ctgaacttag taaaagacca acaggaaaga ctttgtatat attagatgaa
    10681 cctactactg gacttcatat ggcagatgta gacaagctaa tccaagtatt acaaagacta
    10741 gcagatacag gaaatacaat tatagtcata gaacataatt tagatgttat aaagacttgt
    10801 gattatgtaa tagacttagg accagaaggc ggagataagg gtggaactat agttgcaact
    10861 ggaacaccaa aagaagtatc taaagttgat ggttcttata caggtcaatt cttaaagaaa
    10921 tatttttctt aagttatata aattgtgaaa aattttagag cagatatacg ataaactgtt
    10981 ttgttataag taaattagtc aaatataagt tgtaaatagt aatacaaagt aaagtccata
    11041 ttactcttca tggttaaaat tgttttcaaa tcaattatag aagggaatat gggctttaac
    11101 ttgtttaaag cactggaaat tgtataactt tttaaaattc atattttagg gtataatttt
    11161 agtataaaat ttaatgtttt ggagggcgag atgtttgata tacaagagca tttaaaaaaa
    11221 ttaccttctg agcccggagt atatttaatg aaagacaaat acgaccatat aatatatgtg
    11281 ggaaaagcca tatctctaaa aaatagggta aggcagtatt ttcaatcttc aaaaaaccat
    11341 acatctaaag taaaatctat ggttaagaat atatataagt ttgagtatat tataacggat
    11401 tcagagttag aggcattaat acttgaatgt aatttaataa aaagatacag accaaagtat
    11461 aatgtagtgc ttagagatga taaaacttat ccatatataa aagtaactac gaatgaagat
    11521 tatccaagaa tcttaaaagt tagaagagtt cttaaagata aggcaaagta ttttgggccc
    11581 tataccaata taactgctgt gaatgatacc ttggagctta taagtagtac gtatccaata
    11641 agaagctgta aaatagatat agataaagca ataaagaata agacaagacc ttgtttaaat
    11701 ctacacataa ataaatgttt aggaccttgt acaggaaatg tttctaaaga agagtatgga
    11761 aaaatgatag aagagattat aatgtgttta tctggtaaag aagaaaaatt gatggaactt
    11821 ttaaaggaaa aaatgaatga gagttctatg aactttagat ttgaagaggc tgctgtatat
    11881 agggataaga taaagagcct tgaagagatg atacagaaac aaaaaataga tgctactgta
    11941 agtgacttaa accaagatgt ggttgcaatg gcaagagctc ataatgaagc ttgtgtccag
    12001 gtatttttta taagaaatgg taagattgta ggtagggaac attttatatt agaaggggta
    12061 atggatagtc caagagcttc tatattaagt tcatttgtaa aacaatttta taatgaacaa
    12121 gagtatatac ctaaagaatt aattatagaa gatgagatag aagatagttc tatattagaa
    12181 gaatggctgt catctaaaaa ggggcaaaaa gtaacaataa gagttcctca aaagggagag
    12241 aagaaaagct tagttgaaat ggttaggaaa aatgctgtag aatatcttga aaaattttct
    12301 gatatgaata aaagaaaata tgaaaaaagt attggagcat tggaagaatt aaagcaaata
    12361 ttaaatctag aaaagttgcc aataagaata gaggcttatg atatatcaaa tatacaaggt
    12421 gtagattcga ttggttctat ggtagtgtat acgaatgcta aaaaagataa gaaggaatat
    12481 aggagatata aaataaagac agttattgga cctaatgatt atgattctat ggctgaaatt
    12541 gtagatagaa gattaaaaca tggaaattta cctgacttga tattgttgga tggtggaaaa
    12601 ggacaagtta gtgcagtaaa aaaagtacta gaactcaatg atgtagatat acctctttgg
    12661 ggaatgtaca aagatgataa gcatagaact aaaggattga tatgcaaaga aaaagaaata
    12721 gaactagata aaacaactaa tttatataga tttattgcta gtatacaaga agaagttcat
    12781 aattatgcaa taacatatca tagaagtcta agaaataaag ctttgacaaa atctatctta
    12841 gatgatatac aaggaatagg tgaaaaaaga aagaaatcat tattaaatca ctttaaagat
    12901 gtagatgcta taaaaaaagc tactatggaa gaacttttag aagtagatgg aatgaataaa
    12961 agcattgcag ataatgttta taattttttt agaaaggaag aaaattaatg gaaactagca
    13021 ataaagtatc aataagagag ataataaaag agttgaagtt aaaagtagta tacatgcctg
    13081 atgatgtaga ttattatgtc tactctcaag atataaatag accaggatta cagtttgcag
    13141 gatattttga ttattttgca tatgaaagaa tacaaatagt tggaaagaca gaatataatt
    13201 tttttggatt aatggatagt catattcgag aagaagtact agatagattt ttctcatatg
    13261 aaataccagc gttaataatt tctagagact tggaaatcaa gccagatgta attgaaaagg
    13321 ctaaaaaata taaaagaata cttttgagca caaatagaaa taccactaga cttataaata
    13381 gactgtcaaa ctatttggat aataagttag caccacacac aacaattcac ggggtgctag
    13441 tagatattta tggtataggt gtactgataa aaggtgaaag tagtatagga aagtcggaaa
    13501 cagcattaga actgattcaa aggggtaata gattagttgc agatgatgct gtagaaataa
    13561 gaaaattaga tgaaagtatg cttgtgggtc agtcacctga actaataaga cattttcttg
    13621 agataagagg aataggaata atagatgtta gaagtttata tggagtaggg gcaatcaaaa
    13681 attctaaaat gatagattta gtagttcatt tagaagcttg ggatgaaact aagtattatg
    13741 atagactagg actggataaa gaatatgaag aaatacttgg tgttaatgta gagaaattag
    13801 ttgtacctgt taaacctgga agaaatactg ctatgatatt agaagtagca gcaatgaatt
    13861 ttagacaaag aggaatggga tacgatgcag ctcaagaatt tactaaaaaa ttatcaaaat
    13921 taatcgatac taagtagtac tgaataatag tgaactacac atcatttaat taaatattga
    13981 ttaggattat atcctacac
//
LOCUS       DAELLV010000088        13977 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.088, whole genome shotgun sequence.
ACCESSION   DAELLV010000088 DAELLV010000000
VERSION     DAELLV010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13977
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.088"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            417..1616
                     /gene="hadA"
                     /locus_tag="KPD58_002022"
     CDS             417..1616
                     /gene="hadA"
                     /locus_tag="KPD58_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocaprenoyl-CoA:2-hydroxyisocaproate
                     CoA-transferase HadA"
                     /protein_id="HBG0265788.1"
                     /translation="MLLEGVKVVELSSFIAAPCCAKMLGDWGAEVIKIEPIEGDGIRV
                     MGGTFKSPASDDENPMFELENGNKKGVSINVKSKEGVEILHKLLSEADIFVTNVRVQA
                     LEKMGIAYDQIKDKYPGLIFSQILGYGEKGPLKDKPGFDYTAYFARGGVSQSVMEKGT
                     SPANTAAGFGDHYAGLALAAGSLAALHKKAQTGKGERVTVSLFHTAIYGMGTMITTAQ
                     YGNEMPLSRENPNSPLMTTYKCKDGRWIQLALIQYNKWLGKFCKVINREYILEDDRYN
                     NIDSMVNHVEDLVKIVGEAMLEKTLDEWSALLEEADLPFEKIQSCEDLLDDEQAWAND
                     FLFKKTYDSGNTGVLVNTPVMFRNEGIKEYTPAPKVGQHTVEVLKSLGYDEEKINNFK
                     DSKVVRY"
     gene            1655..2455
                     /gene="hadI"
                     /locus_tag="KPD58_002023"
     CDS             1655..2455
                     /gene="hadI"
                     /locus_tag="KPD58_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyisocaproyl-CoA dehydratase activator
                     HadI"
                     /protein_id="HBG0265789.1"
                     /translation="MYTMGLDIGSTASKGVILKNGEDIVASETISSGTGTTGPSRVLE
                     KLYGKTGLAREDIKKVVVTGYGRMNYSDADKQISELSCHARGVNFIIPETRTIIDIGG
                     QDAKVLKLDNNGRLLNFLMNDKCAAGTGRFLDVMAKIIEVDVSELGSISMNSQNEVSI
                     SSTCTVFAESEVISHLSENAKIEDIVAGIHTSVAKRVSSLVKRIGVQRNVVMVGGVAR
                     NSGIVRAMAREINTEIIVPDIPQLTGALGAALYAFDEAKESQKEVKNI"
     gene            2470..3696
                     /gene="hadB"
                     /locus_tag="KPD58_002024"
     CDS             2470..3696
                     /gene="hadB"
                     /locus_tag="KPD58_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     HadB"
                     /protein_id="HBG0265790.1"
                     /translation="MSEKKEARVVINDLLAEQYANAFKAKEEGRPVGWSTSVFPQELA
                     EVFDLNVLYPENQAAGVAAKKGSLELCEIAESKGYSIDLCAYARTNFGLLENGGCEAL
                     DMPAPDFLLCCNNICNQVIKWYENISRELDIPLIMIDTTFNNEDEVTQSRIDYIKAQF
                     EEAIKQLEIISGKKFDPKKFEEVMKISAENGRLWKYSMSLPADSSPSPMNGFDLFTYM
                     AVIVCARGKKETTEAFKLLIEELEDNMKTGKSSFRGEEKYRIMMEGIPCWPYIGYKMK
                     TLAKFGVNMTGSVYPHAWALQYEVNDLDGMAVAYSTMFNNVNLDRMTKYRVDSLVEGK
                     CDGAFYHMNRSCKLMSLIQYEMQRRAAEETGLPYAGFDGDQADPRAFTNAQFETRIQG
                     LVEVMEERKKLNRGEI"
     gene            3696..4823
                     /gene="hadC"
                     /locus_tag="KPD58_002025"
     CDS             3696..4823
                     /gene="hadC"
                     /locus_tag="KPD58_002025"
                     /EC_number="4.2.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     beta"
                     /protein_id="HBG0265791.1"
                     /translation="MEAILSKMKEVVENPNAAVKKYKSETGKKAIGCFPVYCPEEIIH
                     AAGMLPVGIWGGQTELDLAKQYFPAFACSIMQSCLEYGLKGAYDELSGVIIPGMCDTL
                     ICLGQNWKSAVPHIKYISLVHPQNRKLEAGVKYLISEYKGVKRELEEICGYEIEEAKI
                     HESIEVYNEHRKTMRDFVEVAYKHSNTIKPSIRSLVIKSGFFMRKEEHTELVKDLIAK
                     LNAMPEEVCSGKKVLLTGILADSKDILDILEDNNISVVADDLAQETRQFRTDVPAGDD
                     ALERLARQWSNIEGCSLAYDPKKKRGSLIVDEVKKKDIDGVIFCMMKFCDPEEYDYPL
                     VRKDIEDSGIPTLYVEIDQQTQNNEQARTRIQTFAEMMSLA"
     gene            4871..6004
                     /locus_tag="KPD58_002026"
     CDS             4871..6004
                     /locus_tag="KPD58_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HBG0265792.1"
                     /translation="MLYNKEQELLRKAVRDFVSKELDTLPAEMDKTGVMPKELIKKLA
                     DAKFISSNIPEEYGGGGAGYVSYAIVMEEIARRCASTATFVTAGSSLASLPILYNGTE
                     EQKQKYLKGIATGELIGAFGLTEPGAGSDAGGQQTTAELVGDHYILNGRKTFITNGPF
                     CDVAIVIAVTDRSKGLRGTSAFIVESKWDGFSTGAHEDKMGIRGTETSDLIFENVKVP
                     KENLLGKEGQGFKIAMGTLEVGRIGVAALALGIAQGALDEAVKYTKQRVQFGKPIAKF
                     QNTQFTIADMETKVCAARGLVYDAAQKRDAGMRVAQESAMAKYYASEIANEVAYKALQ
                     LHGGYGFIKDYEIERMYRDARIVSIYEGTSEVQKMVISSNVLK"
     gene            6037..6822
                     /locus_tag="KPD58_002027"
     CDS             6037..6822
                     /locus_tag="KPD58_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBG0265793.1"
                     /translation="MKILVCVKQVPDTNEVRINKETGTLIRDGVPSILNPDDANALEE
                     ALKIKDEHDDVTITVITMGPPQADFMLRECLAMGADDAILLSDRAFGGADTWATSNTI
                     AAGISKVGDYDIIFAGRQAIDGDTAQVGPQIAEKLDIPQVTYVQDFKIEGKDIIVQRQ
                     LEDGYELIKVSTPVLLTAVKELNTPRYMSVDKIVKAYKHDVKVWTIDDLDVNKEEVGL
                     KASPTKVFRSFTPEPRGKGEILEGKADEIASKIIIGLKQKHII"
     gene            6841..7878
                     /locus_tag="KPD58_002028"
     CDS             6841..7878
                     /locus_tag="KPD58_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBG0265794.1"
                     /translation="MNDIKDLSSYKNVWIFAEQREGKIAPVVIELLGEGRKLAKEVDA
                     ELCAILLGKDVDGLAKELITFGADKVYVADDALLEKYTTDAYTKVIKDAIDEIKPEIM
                     LFGATHIGRDLAPRIASRVGTGLTADCTKLEIDPEDKKIKQTRPAFGGNIMATIICPN
                     HRPQMSTVRPGVMDKAEKDETRTGEVIALDYKITQDDIRTTVLETVKTKKDLVSLTDA
                     NVIVSGGLGLGGPEGFEMLKKLADKLGGVVGSSRAAVDAGWIDHSHQVGQTGTTVKPN
                     LYIACGISGAIQHLAGMQSSDFIIAINKNPAAPILEIADYGVVGDLHEIVPMLIEKLD
                     SVDDLLEAIKA"
     gene            8361..9992
                     /locus_tag="KPD58_002029"
     CDS             8361..9992
                     /locus_tag="KPD58_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0265795.1"
                     /translation="MDNNLIGLIEHVENPAILCKESGEIIYCNHLIDSIFSFLDIKKP
                     RNINELDSNFDKTEILTDSKKKIAFRELRMTAHIYNMKDNNNENNIVYLFEKSLISDK
                     VIEDIIEHIDEVVVVFNKDGVIEKMNTVSDEILPFKRTEVLGRNITDLVRQGLVEEPI
                     ILNMLKVKKKIYRNIVYPDGKLIAYTAVPRWDSKGKLTGGVLTGRDISRVIKLESQIK
                     YSDISEDTEYISQSKIMDNIKKVVKRAAASDSSIFINGESGVGKEIIARTIYKYSSRR
                     DKPFIAINCGAIPNELLESEFFGYEEGSFTGAKKKGKKGLFEEANGGTIFLDEIGELP
                     MQMQKKLLRVIQENTITRIGGSKPIKIDVRYISATNISHEDLRNNLKFRQDLYYRLSV
                     IPVKIPPLRERKEDIVPLVNYFLKLYNEKYNREVEVSPKVIELLEEYSWPGNIRELKN
                     IIERFVVLSAKNVIGEDEFNMLINLDMIDNETDDLSPIVVNGIMNLNDAYKIVDQIMI
                     SKAINKYGSITKAAEVIGIYPSTIHRKIKSGHIHV"
     gene            complement(10127..11053)
                     /gene="fba"
                     /locus_tag="KPD58_002030"
     CDS             complement(10127..11053)
                     /gene="fba"
                     /locus_tag="KPD58_002030"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HBG0265796.1"
                     /translation="MALITTKEMFKKAYEGGFAIGAFNISDLEQLQGVLKAAKAKNSY
                     VMIQASMSAVKYAGPHTLVEMVKAASDEIGVDVALHLDHGPNMDAIKTCIDAGFSSVM
                     IDGSHFDFEENVRITKEAVEYAHSKGVVVEAELGVLAGTEDDVTSDVHKYTQPAEAVE
                     FVERTGVDSLAIAIGTSHGAFKFKGEAKLRFDILEEIQSKLPGFPIVLHGASAVDQNA
                     VATCNEFGGNIAGAKGVPVDMLRKASSMAVCKINMDTDLRLAMTAAIRKFLAENPKEF
                     DPRKYIGAGRDAIQAVVESKIDDVLGSANSIN"
     gene            11383..11712
                     /locus_tag="KPD58_002031"
     CDS             11383..11712
                     /locus_tag="KPD58_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1904 domain-containing protein"
                     /protein_id="HBG0265797.1"
                     /translation="MPQIKIRGINENDICKISEKMINDLVEAVKCPRDYFEIECIKSV
                     AIRDGKIADVYPFVEVAWFDRGQEVQDIVARIITDSIRNNLDVESIDLAFTVFEKEKY
                     YENGEHF"
     gene            11712..12434
                     /locus_tag="KPD58_002032"
     CDS             11712..12434
                     /locus_tag="KPD58_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBG0265798.1"
                     /translation="MAKKQRIDKILSNLGYGSRSEIKKYCKQGSVVVNGSEVSNPGTQ
                     VDTENDEILFNGEEVIYREYIYLMMNKPDGYISATTDKYDPTVLDLIDLSYLAFEPFP
                     VGRLDKDTEGLLVLTNDGKLSHRVLSPKKHVPKTYYAKIDGVVTEEDVEAFLEGVVLD
                     DGYKTMPSQLNILKSDDESEIELTIHEGKFHQVKRMFESVGKKVVYLKRLSMGNLKLD
                     ESLELGEYRELTDEEVKLIEER"
     gene            12461..13822
                     /gene="rlmD"
                     /locus_tag="KPD58_002033"
     CDS             12461..13822
                     /gene="rlmD"
                     /locus_tag="KPD58_002033"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBG0265799.1"
                     /translation="MSVKKKDVIEFEVDKMEFGGTSLSQVGDRVIHMKGGISGQKVRA
                     GVKKVRSKKAEVKMIELLENSPLETEETCKHFRECGGCTLLSVPYEKQLEIKEKQVMD
                     LFLKQDLFGFQFLGIEGSPENKYYRNKMEYTFGDEVKNGPLTLGLHKKGKHIDIQTVE
                     ECMLVDEDFSKILVSSVEFFNEKKLPYYRTMNHKGYLRHLVVRKGIHTNEIMVNIVTS
                     SQEDFDMYEFKDMLLGIDLKAELVGVLHTINDGLADAVQCDELRVLYGRDYIQEEILG
                     LKFKISPFSFFQTNTKGAEVLYSIARDFIGDYNDKVVFDLYSGTGSIGQVMAGAAKKV
                     YGIEIVEEAVVAANENAKLNGLTNCEFIAGDVAKVVKDLKDKPDLIIVDPPRPGIHKD
                     AIRDICGFGAKEIVYISCNPKSLVVDLVDFKGYGYEIKMVKCMDMFPNTPHCETVVLL
                     RRK"
BASE COUNT         5496 a         1631 c         2711 g         4139 t
ORIGIN      
        1 tgctgattaa ttacaatgat aacatgtgtt tttgagttta gcaatgaaaa ctttttttga
       61 aatgtcaagc tttttcataa aaaaatatat aaaaaaaact tttataagca tagaaagaat
      121 agcaaaatgc aatggatagt ggcaagatgc cacgatatga aaaagattat tcaattgcat
      181 attgttaaaa aacattcaaa tcaagcgata ttttgattaa atataaagtt ggcacgataa
      241 atgcttaaca atataggtgt aagacaataa aacaataaaa aataatttta ataattttta
      301 agttatttta gataagatta ggaactttga catcagacag ttaatatcag aaaaaagtat
      361 tttatgagtg atttaatcga aattttataa ttgatactat taaggaggaa aacgttatgc
      421 ttttagaagg agttaaagta gtagaacttt caagtttcat cgcagcacca tgttgtgcaa
      481 aaatgttagg tgactggggt gcagaggtta ttaagattga acctatagaa ggtgatggaa
      541 taagagttat gggtggaaca tttaaatctc cagcatcaga tgatgaaaac cctatgtttg
      601 aattagaaaa tggaaataaa aagggtgtaa gtattaatgt aaaatcaaaa gaaggagtag
      661 aaatattaca taaattatta tcagaagcag acatatttgt aactaatgtt agagttcaag
      721 cattagaaaa aatgggtata gcttatgacc aaataaaaga taagtatcca ggattaatat
      781 tctctcaaat attaggatat ggtgaaaaag gacctttaaa agataaacca ggatttgact
      841 atactgcata cttcgcaaga ggaggagtta gccaatctgt tatggaaaaa ggaacatctc
      901 cagcaaatac agcagcagga tttggtgacc actatgcagg tctagcacta gcagcaggaa
      961 gtttagcagc attacataaa aaagctcaaa ctggtaaagg tgagagagta acagtaagtc
     1021 ttttccatac agctatatat ggaatgggaa caatgataac aacagcacaa tacggaaatg
     1081 aaatgccttt atcaagagaa aatccaaaca gcccattaat gactacatat aaatgtaaag
     1141 atggaagatg gattcaatta gctttaatac aatacaacaa gtggttaggc aaattctgta
     1201 aggttataaa tagagaatat atattagaag atgatagata taataacata gattcaatgg
     1261 ttaatcatgt tgaagattta gttaagatag ttggagaagc tatgttagaa aaaacattag
     1321 acgagtggtc agctttatta gaagaagcag acttaccatt tgaaaaaatt caaagctgtg
     1381 aagatttatt agatgacgaa caagcttggg caaatgactt cttatttaag aaaacatacg
     1441 atagcggaaa tacaggtgtc ttagttaata ctccagttat gtttagaaat gaaggaatta
     1501 aagaatatac accagcacca aaagtaggtc aacatactgt agaagtatta aaatctttag
     1561 gctacgatga agagaaaata aataacttta aagatagtaa agttgtaaga tattaattag
     1621 taattctaga ttttttaaaa ggagaatgct aaagatgtac acaatgggat tagatatagg
     1681 ttcaactgca tcaaagggag taatcttaaa gaatggggaa gatattgtag cttctgaaac
     1741 aatatcctct ggtactggga ctactggacc atcaagagtt ttagaaaaat tatatggcaa
     1801 gacaggtctt gcaagagaag atattaaaaa agttgtagtt acaggatatg gaagaatgaa
     1861 ctattcagat gctgataagc aaataagtga attaagctgt catgctagag gggtaaattt
     1921 cataattcca gagacaagaa ccattattga cataggtggt caagatgcaa aggtattaaa
     1981 attagataat aatggaagac tattaaactt tcttatgaat gacaaatgtg ctgcaggtac
     2041 aggaagattt ttagatgtaa tggcaaaaat aatagaggtt gatgtatctg aactcggaag
     2101 tatatctatg aattctcaaa atgaagtatc aataagcagt acatgtacag tatttgcaga
     2161 gtctgaggtt atatcacatt tatctgaaaa tgcaaaaatt gaagatatag tggcaggtat
     2221 tcatacttca gtagcaaaga gagtttctag cctagtaaaa agaataggag tacaaagaaa
     2281 tgtagttatg gttggtggag ttgctagaaa tagtggtatt gtaagagcta tggcaagaga
     2341 aatcaacaca gaaattattg tacctgatat acctcaatta actggtgctt taggagcagc
     2401 gttatatgct tttgatgaag caaaagaatc acaaaaagaa gtgaaaaata tataaaaaga
     2461 gggatgaaaa tgtctgaaaa aaaagaagct agagtagtaa ttaatgattt attagctgaa
     2521 caatatgcaa atgcatttaa agctaaagaa gaaggaagac ctgtaggttg gtcaacatca
     2581 gtatttcctc aagagttagc agaagtattt gacttaaacg tattatatcc agaaaaccaa
     2641 gcagctggag tagcagctaa aaaaggttct ttagaattat gtgaaatagc tgaatctaaa
     2701 ggatattcta ttgacctatg tgcatatgca agaacaaatt ttggtctttt agaaaatggt
     2761 ggatgtgaag ctttggatat gccagctcca gatttcctac tttgctgtaa caatatatgt
     2821 aaccaagtta taaaatggta tgaaaatatt tcaagagaat tagatatacc tttaataatg
     2881 attgatacaa ctttcaataa tgaagacgaa gttactcaat caagaataga ttatattaaa
     2941 gctcaatttg aagaagctat aaaacaacta gaaattatat caggaaagaa atttgaccct
     3001 aagaagtttg aagaagtaat gaaaatatca gctgaaaacg gaagactatg gaagtattct
     3061 atgagtttac cagcagattc ttctccttct ccaatgaatg gatttgactt atttacttac
     3121 atggctgtaa tagtttgtgc tagaggtaaa aaagaaacta cagaagcatt taagttactt
     3181 atagaagaat tagaggacaa catgaaaact ggtaaatctt ctttcagagg ggaagaaaaa
     3241 tacagaataa tgatggaagg tataccttgt tggccatata taggatacaa gatgaaaaca
     3301 ttagctaaat ttggagttaa catgacaggt agtgtttacc cacatgcttg ggcattacaa
     3361 tatgaagtta atgatttaga tggaatggca gtagcatata gtactatgtt taacaatgta
     3421 aacctagacc gtatgacaaa atatagagtt gattctttag tagagggtaa atgtgatgga
     3481 gcattctatc atatgaacag aagctgtaaa cttatgagtt taatacaata tgaaatgcaa
     3541 agaagagcag ctgaagaaac tggattacca tatgctggat ttgatggtga ccaagcagac
     3601 cctagagctt tcactaatgc tcaatttgaa acaagaattc aaggtttagt tgaagtaatg
     3661 gaagaaagaa aaaaacttaa tagaggtgag atataatgga agctatttta tctaaaatga
     3721 aagaagtagt tgaaaatcca aatgcggctg taaaaaaata taaaagtgaa actggtaaaa
     3781 aagctatagg ttgtttccca gtttattgcc cagaagaaat tatacatgca gctggaatgc
     3841 ttccagttgg tatatgggga gggcaaacag aattagattt agctaaacaa tatttccctg
     3901 catttgcatg ttcaataatg caatcatgtt tagaatatgg attaaaaggt gcttatgatg
     3961 aattatctgg agttattata ccaggtatgt gtgatacact aatttgttta ggacaaaact
     4021 ggaaatcagc agtacctcat ataaaatata tatcattagt acacccacaa aatagaaaac
     4081 ttgaagctgg tgtaaaatac ttaatcagtg agtacaaagg cgtaaaaaga gaacttgaag
     4141 aaatttgtgg atatgaaata gaagaagcaa aaattcatga aagtatagaa gtttacaatg
     4201 aacatagaaa aactatgaga gactttgttg aagtagctta taaacattct aatactataa
     4261 aaccatcaat aagaagctta gtaattaaga gtgggttctt tatgagaaaa gaagaacata
     4321 ctgagctagt gaaagattta atagcaaaat taaatgctat gccagaagaa gtctgttctg
     4381 gaaagaaagt tttattaaca ggtatattag ctgattctaa agatatatta gacattttag
     4441 aagacaacaa tatatcagtt gtagctgacg acttagcaca agaaacaaga caattcagaa
     4501 cagatgtacc agcaggtgat gatgcgttag aaagattagc aagacaatgg tcaaacatag
     4561 aaggatgttc attagcttat gaccctaaga aaaaacgtgg gtcacttata gtagatgaag
     4621 ttaaaaagaa agatatagat ggtgttatct tctgtatgat gaaattctgt gacccagaag
     4681 aatacgatta tcctttagtt agaaaagata tagaagatag tggaatacct actttatatg
     4741 ttgaaatcga ccaacaaact cagaataatg aacaagccag aactcgtatt caaacttttg
     4801 ctgagatgat gagtttagcg taagttcatt aaaataaatt tactataaaa tatgaaaatt
     4861 ggaggaagct atgttatata acaaagaaca agaactttta agaaaagcag taagagattt
     4921 tgttagtaaa gaattagata ctttaccagc agaaatggat aaaacaggtg taatgcctaa
     4981 ggaattaatc aaaaaattag ctgatgcaaa atttataagc agtaatatcc cagaagaata
     5041 tggtggtggg ggagcaggat atgtttctta cgctatagta atggaagaaa tagctagaag
     5101 atgtgcttca acagctactt ttgttacagc tggttcttct cttgcttcat taccaatact
     5161 ttacaatggt actgaagagc aaaaacaaaa atacttaaaa ggtatagcaa caggtgaatt
     5221 aataggagca tttggtttaa ctgagccagg agctggttct gatgcaggtg gacaacaaac
     5281 aacagcagaa ttagttggag accactatat attaaatggt agaaaaactt tcataactaa
     5341 tggaccattc tgtgatgtag ctatagtaat agcagttaca gatagaagta aaggacttag
     5401 aggaacatca gcgtttatag tagaaagtaa atgggatggt ttctcaacag gagctcatga
     5461 agataagatg ggtataagag gaactgaaac ttctgactta atatttgaaa acgttaaagt
     5521 tccaaaagaa aacttacttg gaaaagaagg tcaaggattt aagatagcaa tgggtactct
     5581 agaagttggt agaataggtg ttgctgcctt agctctagga atagctcaag gtgctttaga
     5641 tgaagctgtt aaatatacaa aacaaagagt tcaatttggt aagcctatag ctaaattcca
     5701 aaatactcaa tttactatag ctgacatgga aacaaaagtt tgtgcagcta gaggattagt
     5761 ttatgatgca gcacaaaaga gagatgcagg aatgagagtt gctcaagaat ctgctatggc
     5821 taaatactat gcatcagaaa ttgcaaatga agttgcttat aaagcattac aacttcacgg
     5881 tggatatgga tttataaaag attatgaaat cgaaagaatg tacagagatg ctagaatcgt
     5941 atcaatatac gaaggaacat cagaagttca aaaaatggta atttcatcaa acgtattaaa
     6001 ataataaata atctaatgaa attggaggta tagactttga aaatattagt ttgcgtaaaa
     6061 caagttccag atactaacga agtaagaata aataaagaaa caggtactct tataagagat
     6121 ggagttccaa gtatattaaa cccagatgat gcaaatgcat tagaggaagc tttaaaaatt
     6181 aaagatgagc atgatgatgt aactataaca gttataacaa tgggacctcc tcaagcagac
     6241 tttatgctaa gagagtgttt agctatgggt gctgatgatg caatattatt aagtgataga
     6301 gcatttggag gagctgatac ttgggctaca tcaaatacta ttgcagcggg aatatctaaa
     6361 gtaggtgact atgacataat atttgcagga agacaagcaa tagatggaga tactgcacaa
     6421 gttggaccac aaattgcaga gaaattagac attcctcaag ttacatatgt acaagatttc
     6481 aaaatagaag gaaaagatat aatagttcaa agacaattag aagatggata cgaattaata
     6541 aaagtaagta cacctgtact tttaacagca gtaaaagagt taaatacacc tagatacatg
     6601 tctgttgata aaatagtaaa agcatataaa catgatgtaa aagtttggac aattgatgat
     6661 ttagatgtaa ataaagaaga agttggttta aaagcatcac caactaaagt atttagatca
     6721 tttactcctg aaccaagagg aaaaggagaa atcttagaag gtaaagctga tgaaatagct
     6781 agtaaaataa taatcggtct aaaacaaaag cacattatat aagttggagg agtacaaact
     6841 atgaatgata taaaagattt aagttcttat aaaaacgtat ggatatttgc agaacaaaga
     6901 gaaggaaaaa tagctccagt agttatagaa ttattaggag aaggaagaaa attagctaaa
     6961 gaagtagatg cagaactttg tgcaatatta ttaggaaaag atgttgatgg attagctaaa
     7021 gaattaatca cttttggagc tgacaaagtt tatgttgcag atgatgctct tttagaaaaa
     7081 tatacaactg atgcatatac aaaagtaata aaagatgcaa tagatgaaat aaaaccagaa
     7141 ataatgcttt tcggagcaac tcatataggt agagacttag cacctagaat agcttcaaga
     7201 gttggaactg gattaacagc tgactgtact aagttagaaa tagacccaga agataagaaa
     7261 ataaaacaaa ctcgtccagc atttggtgga aacataatgg ctacaatcat ttgtccaaac
     7321 catagacctc aaatgtctac agttagacct ggtgttatgg ataaagctga aaaagacgaa
     7381 acaagaactg gtgaagttat agcattagac tacaaaataa ctcaagatga tataagaact
     7441 actgttttag aaacagttaa aactaagaaa gatttagtat ctcttacaga tgcaaatgtt
     7501 atagtatcag gtggtctagg attaggtgga ccagaaggat ttgaaatgct taagaaatta
     7561 gctgacaaat taggtggagt agttggttct tctcgtgcgg ctgttgatgc tggatggata
     7621 gaccattctc accaagtagg tcaaacagga actactgtta aaccaaacct atatatagct
     7681 tgtggtatat caggagcaat acaacattta gcaggtatgc aatcatcaga tttcataatt
     7741 gctataaaca aaaacccagc agctccaatt ttagaaatcg ctgactatgg agtagttgga
     7801 gacttacatg aaatagttcc aatgcttata gaaaaattag atagcgttga tgatttatta
     7861 gaagctataa aagcttaata gatacaaact atataaatat tactgattga ataagtctta
     7921 ttagttagta acataattat taaccctttg ataagttatt gctagtgtgt atttgtcagt
     7981 tattaaaaag tctgtttgaa taaaatttta taatacaaga taatctggat aatccttata
     8041 tgtatattgc ttacaaattg tgagttatat tctgtaagga ttatctttgt ttttttaaat
     8101 aaatcttaaa tttaattaaa ttgtattaaa tagacaaata attactaaat atgtgataaa
     8161 atacaattaa aagcaaaatg aaatttttaa caaactataa tcattaaaat tatatcttag
     8221 ttacgtttta atgaaataaa ctacttgaaa agacgaatat tataaggagt tgattatatg
     8281 tgctgatggt atttattgta agcaaacaat aaattttgta tgtagttcaa taaattactt
     8341 aatgaaaggt gcgaatatta atggataata atttaatagg gcttatagaa catgtagaaa
     8401 atccagccat tttatgtaaa gaatcaggcg aaataatata ctgtaatcat ctaatagata
     8461 gtatttttag ttttctagat ataaaaaagc ctagaaatat aaatgaatta gattcaaatt
     8521 ttgacaaaac agaaatactg acagatagta aaaagaaaat agctttcaga gagttaagga
     8581 tgactgcaca tatatacaat atgaaagata ataataatga aaataatata gtttatttat
     8641 ttgagaagtc tctaatatct gataaagtaa ttgaagatat aatagagcat atagatgaag
     8701 ttgttgttgt gtttaacaaa gatggggtaa ttgaaaagat gaacactgtc agtgatgaga
     8761 tattaccttt taaaagaact gaggtacttg gtagaaatat aacagacctt gtgagacaag
     8821 gattagttga agaaccaata atattaaaca tgcttaaagt gaagaaaaag atttatagaa
     8881 atatcgttta cccagatggg aagcttattg catatacggc agttccaaga tgggattcta
     8941 agggaaagct tacaggagga gtactaacag ggagagatat ttcaagggtt attaagcttg
     9001 aatctcagat aaaatatagt gatatatcag aagacacaga gtatataagt caaagtaaga
     9061 ttatggacaa tataaaaaaa gttgttaaga gagctgcagc ctcagattct tctatattta
     9121 taaatggaga atcaggtgta ggaaaagaaa taatagcaag aacaatatat aagtatagtt
     9181 caaggagaga caaacctttt atagctataa actgtggagc tataccaaat gaattattag
     9241 agtcagagtt ttttggatat gaagaaggtt cttttactgg agctaagaaa aaaggtaaaa
     9301 agggactttt tgaagaggcg aatggtggaa ctattttctt ggatgagata ggtgagttac
     9361 caatgcagat gcaaaaaaaa ttacttaggg ttatacaaga aaatactatt actagaatag
     9421 gtggtagtaa gcctataaaa atagatgtta gatatataag tgctactaat atttctcatg
     9481 aagaccttag aaataacctt aaatttagac aagatttgta ctatagatta agtgttattc
     9541 cagtaaaaat acctcctctt agagaaagaa aagaggatat agtaccatta gtaaattatt
     9601 tcttaaaact ttacaatgaa aagtacaata gagaagtaga agtttcgcct aaagttattg
     9661 agttattaga agaatattct tggccaggaa acataagaga attaaaaaac atcattgaaa
     9721 gatttgtagt attatcagct aaaaatgtaa ttggagaaga tgaatttaac atgctgataa
     9781 atttagatat gattgataat gaaacagatg atttatcacc aatagtggta aatggaatta
     9841 tgaatcttaa tgatgcatat aaaatagtgg accaaataat gataagtaag gctataaata
     9901 agtatggttc aataacaaag gctgctgaag ttataggtat atatccttct accatccata
     9961 gaaagataaa gagtggacat attcacgttt agctgtcagt ttaaaaagac aatataatta
    10021 aaatattata agatacataa tttatacaac ttaaaagaga tggcttaaaa ataaatttaa
    10081 ttactttaag tcatctcttt tcttttataa atatttaaaa gttatattag tttatagagt
    10141 tagcagaacc taaaacatca tctatcttgc tttcaactac agcttgtata gcatctctac
    10201 cagcaccaat gtattttctt gggtcaaatt cttttggatt ttcagctaag aattttctga
    10261 tagcagcagt catagctagt cttaagtcag tatccatgtt tattttacaa acagccatag
    10321 aagatgcttt tcttaacata tctacaggta cacctttagc accagctata tttccaccaa
    10381 attcattaca tgtagcaaca gcgttttggt caacagcaga tgcaccatga agaactattg
    10441 ggaaacctgg taatttgctt tggatttctt ctagtatatc gaatcttaat ttagcttctc
    10501 ctttgaattt gaaagcacca tgagaagttc caatagctat tgctaatgaa tcaacacctg
    10561 ttctttcaac gaactcaact gcctcagctg gttgagtata cttgtgaaca tcagaagtaa
    10621 catcatcttc tgttccagct agaacaccaa gttctgcttc aacaacaaca cctttagagt
    10681 gtgcatattc aacagcttct ttagttattc taacattttc ttcaaagtca aaatgagaac
    10741 catctatcat aactgaagag aaaccagcat caatacaagt tttaatagca tccatatttg
    10801 gaccatggtc taagtgaaga gctacatcta cacctatttc atcagaagca gccttaacca
    10861 tttcaactaa tgtatgaggt ccagcatatt taacagctga catagaagct tgtatcataa
    10921 cataagaatt tttagcttta gcagctttta aaacaccttg taattgttct aagtcactta
    10981 tgttaaatgc acctatagca aatccacctt cgtaagcttt tttaaacatt tcttttgtag
    11041 taattaatgc cattttaaac tcctcctatt aacttttaaa atttacccat cacttatatt
    11101 ataacgttaa atcaaaaaaa gtatatagta aattgtaata actataaatt acaagtataa
    11161 tgttaaatag aagacaatag gattaaataa aataataaag aatataaaca atttaggaaa
    11221 actagctata taatgctaac tataagggta ggaactacct tattagtttg gagatactga
    11281 taacaatagt tgccttgacc aagaagttac cacttcaaac gttatcgaaa ggaattaagt
    11341 ggatgataat ttactaaaaa tttaaaataa aggagataaa ctatgccaca gataaaaata
    11401 agaggaatta atgaaaatga tatatgtaaa ataagtgaaa aaatgataaa tgatttagtt
    11461 gaagctgtaa aatgtccaag agactacttt gaaatagaat gtataaagtc agttgcaata
    11521 agagatggta aaatagcaga tgtatatcct tttgttgaag tagcttggtt tgatagagga
    11581 caagaagtac aagatatagt agcaagaata ataactgata gtataagaaa taatttagat
    11641 gtagaaagta tagatttagc atttacagta tttgaaaaag aaaaatatta tgagaatgga
    11701 gagcattttt aatggctaaa aagcaacgta tagataaaat tctttcaaat ttaggttatg
    11761 gaagtagaag tgaaataaag aagtattgta aacaagggtc tgtagtagta aatggaagtg
    11821 aagtttcaaa tccaggaact caagtagaca ctgaaaatga tgagatatta tttaatggag
    11881 aagaagttat atatagagaa tatatatatt tgatgatgaa taaacctgat ggatatatat
    11941 cagcaactac agataaatat gacccaacag tattagattt aattgattta tcatacttag
    12001 cttttgaacc atttcctgtt ggaaggttag ataaagacac agagggatta ttagttttaa
    12061 ctaatgatgg aaagctttct catagagttt tatctcctaa gaagcatgtg cctaagactt
    12121 attatgcaaa aattgatgga gtagttactg aagaagatgt agaagctttt ttagaaggtg
    12181 tagtgcttga tgacggatat aaaactatgc catcacagtt aaatatatta aaaagtgatg
    12241 atgaatcaga aatagagctt acaatacatg aaggtaagtt tcaccaagta aaaagaatgt
    12301 ttgaaagcgt aggcaaaaaa gtagtatact taaaaagatt gtctatgggc aatttaaaat
    12361 tggatgaaag tttagagtta ggtgagtata gagagttaac agatgaagaa gtaaaattga
    12421 ttgaagaaag ataataaaga tactatagta aattggagga atgtctgtga agaaaaaaga
    12481 tgttatagaa tttgaagtag ataaaatgga atttggtggt acatctttaa gccaagttgg
    12541 agatagagtt atccatatga agggtggaat aagtggtcaa aaggtgagag ctggagttaa
    12601 aaaagttaga agtaagaaag ctgaagttaa gatgatagaa ttattagaga actcaccact
    12661 tgaaactgaa gaaacttgca agcactttag agagtgtgga ggatgtacac tattgtctgt
    12721 gccatatgaa aaacaattag aaataaaaga aaaacaagtt atggatttat ttttaaagca
    12781 agatttattt ggatttcagt ttttagggat agaaggaagt ccagaaaaca aatattatag
    12841 aaataagatg gaatatactt ttggagatga agtaaaaaat ggacctttga cattaggtct
    12901 tcataaaaaa ggaaagcata tagatataca aactgttgag gagtgtatgt tggttgatga
    12961 agacttctca aagatattag tttctagtgt agagtttttc aatgaaaaaa agcttcctta
    13021 ttatagaaca atgaatcaca aaggatattt gagacatctt gtagttagaa aagggattca
    13081 tactaatgag attatggtta atatagtaac ttcttctcaa gaagattttg atatgtatga
    13141 gtttaaagat atgcttttag ggattgattt aaaagctgag ttagtaggtg tacttcatac
    13201 tattaatgat ggtttagcag atgcagtcca atgtgatgag ttaagagtat tgtatggaag
    13261 agattatata caagaagaaa ttttaggtct taagtttaag atttcaccat tttcgttttt
    13321 ccaaaccaat actaagggag cagaggttct ttatagtatt gctagggatt ttattggaga
    13381 ttacaatgat aaagttgtat ttgacttgta tagtggtact gggtctattg gacaggttat
    13441 ggctggtgct gctaagaaag tgtatggtat tgagatagtt gaggaagctg ttgttgctgc
    13501 taatgagaat gctaagttga atggacttac taattgtgag tttattgctg gggatgtggc
    13561 taaggttgtt aaggatttaa aggacaagcc tgatttgatt attgttgacc ctcctagacc
    13621 aggcattcat aaggatgcta ttagggatat ttgtggattt ggtgctaagg agattgttta
    13681 tatttcttgt aatcctaagt ctttggttgt tgatttggtt gattttaagg ggtatggtta
    13741 tgagattaag atggttaagt gtatggatat gttcccaaat acgccacatt gtgagactgt
    13801 agtgttgcta cgtcgaaaat aatgctagaa atcttgagct ttagacagtt tcatagacat
    13861 ataatattta gattagaggg taataaaatt aggaataaag aattgaaaag gtgttttgat
    13921 gtgtctgtag ttatatatat ggagtgtggg aatacgaaat aaaaaaagtt ttaatta
//
LOCUS       DAELLV010000089        13951 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.089, whole genome shotgun sequence.
ACCESSION   DAELLV010000089 DAELLV010000000
VERSION     DAELLV010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13951
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.089"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            144..845
                     /locus_tag="KPD58_002034"
     CDS             144..845
                     /locus_tag="KPD58_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HBG0265800.1"
                     /translation="MKFQEMINSPGLWIVSSFLVIISVVQAIVFMKEALKEASNLGIE
                     RKGIKAAIRSASVTAIGPSLSPVITLLSLVAVIGAPTTWMRLCDVGAARTELGVISLT
                     SNLSGVEVGSAAFGAEAFSYALWGMALNNLGWLFVVFILGHRMRGIVEKMNMKYNPTW
                     VKKLLAGATVGLFAYLLSNQIKTFEIPKLTPAIISAIVMLVLTTLFKKNQRLQELSLG
                     IAMLIGMFGTQIILL"
     gene            871..1527
                     /locus_tag="KPD58_002035"
     CDS             871..1527
                     /locus_tag="KPD58_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265801.1"
                     /translation="MYDKKMIKIGRISIGLAIIANFIPAIYVGIRFGQMPMLSVIFQI
                     WGLVAATYGVSWIVQPIAYYPTLGAPGSYIGWLAGSVGDIRMPAASMAQKIAGVEAGT
                     HEGEVVGTIGTCCSIFVSASMITIFTIVGYKVIPLLPEFVTNSFNYILPALFGAIYVD
                     IARKDIRAGGCTIAVALAIMYFGEKIGIPGGLLTLFIVLGGILITRVFFVIDNNKTKE
                     "
     gene            1632..3272
                     /locus_tag="KPD58_002036"
     CDS             1632..3272
                     /locus_tag="KPD58_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HBG0265802.1"
                     /translation="MFDVKIVNGIIVDGTGNSRYKSDVGIVGDKIMAIGDLSQKEAKE
                     TIDATGKFVSPGFIDFHTHSDLSLVYDKYTRSRIHTGVTTDVIANCGIGVAPIREEKK
                     QELIDYLGTRIIGTIPTKLELHWNTMQEYFDYLTENSPAVNVVAYVAQGPVRINEMGF
                     SKEPATLEQLKNMKLEVRKAMEAGCVALSSGLVYLPGAYTKKEEMAELCKELIPYNGY
                     YISHIRDEGDEEMEALDEAIYIAKTAGVPLHVSHLKVMGHKNFGTIDEVFKKLDEAEA
                     DGLEVTFDCYPYTAGMTSLGALLPPWAFEGGVENMVKRLEVQENRDRIIKELEEGIPG
                     WQCFYQLAGGWNGVVLASVMTEANKYVEGKTLMEVAKINGENPFDTFFRLLIEEKSKI
                     QVVVHTQGQEDTDKVVCHPKSCIGSDSMDLSTEGLLSLGKPHPRAFGTFGRIFSYYVR
                     EKGMLTFEEAVKKITYLSAKRLGIYKERGLLKENYFADIVVFDPDTIEDKATYSNPKQ
                     YTVGVEYVLVNGKIALAGGKQTDVCAGRVIKNPLSIAK"
     gene            3630..4952
                     /locus_tag="KPD58_002037"
     CDS             3630..4952
                     /locus_tag="KPD58_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265803.1"
                     /translation="MKILYVGPGMVIDKILAVSKKNFPNIEADFLRYDRYIEVPDLLE
                     KYRGRVDAVLFTGKSPFKFFEKRQKDFVLSDYIPRHETTLYRVLLEITYLLKLDISRL
                     SIDTYDKAMMKRLYKEIGVEFKDEHMFFAEQKYLEEDYIKFVLEFHKYNYQNNDVCCC
                     ITGLEEAYHIMKKDNIPVVLALPSEDVMVQSIKNIQMRYIAKKNNENQIVVLAIKLNM
                     PSEYSLMKEDEYAYLSQRIKILDKLYHFNSRIDGVLVEQSRSEFMIFTTKKILELETK
                     KYRDIYLLDMIREVSVINTYIGIGYGKTANEAKFNAYESIKMAQSQKNDAAYVVFENG
                     EIMGPLKPNPTVKEKNSFDERFYQIATETGLSANTVYKVFNKIINSGKTEFTSKELAT
                     ICGVSIRTMDRIILRLCDSGYCEVVSEQLMSKYGRPSRILRFKPFLLF"
     gene            5149..6156
                     /locus_tag="KPD58_002038"
     CDS             5149..6156
                     /locus_tag="KPD58_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HBG0265804.1"
                     /translation="MGKYQYSKKVVRNYFEALENATGDEVENVLKQHMKSDYNWKGVY
                     PFREQEGTENVADVFWKPLKKSLSNMQRRQDIFIAGTNEIDNSEWVMSMGHFMGLFDN
                     DWLGIKHTRKMISLRYAEFNCVQDGKIAKTGLFVDIIGFMIQAGVNPLPPQTGSYFVY
                     PGPIDHNGLLFEDADEVEGVKTLALVNKMVDDLSELNESGAMGCPPEILEKSWAKDMI
                     WYGPGGIGASYTIPRYQEQHQLPFRNNLKGKTFNGHVCRFAEGNFACFFGWPNLTNVP
                     CGGFLGLPGGEVKADMQVVDVYYRKGDKLQENWVLIDIPYWLKQQGLDILERTKSIFN
                     A"
     gene            6194..7606
                     /locus_tag="KPD58_002039"
     CDS             6194..7606
                     /locus_tag="KPD58_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HBG0265805.1"
                     /translation="MASTNKNNATSKEEEKEVSTSVLESCTVKEKKAKMNQTNFSNIN
                     ENQTNENKTNTYKGNIDKGNIDKTNTNKSDINKVNIDTVDTDKLNTNTLTEKNKKVIF
                     KGEDNMSSTKIYYGDSLEVTPVGVMDYNDFSKQTKREQEIPGFDSKYRDFVDYIMKIT
                     HNIWEEKGIGVIYDTYHNNVTMHCGSSNLVGIKDVISNTLQTLHAFPDRRLIGQNVIW
                     SNFGADGFLSSHRVLSTATNLGDSNFGPATGRKINFRTVIDCATTNNRIHEEWLVRDN
                     LWIVTQLGLNPQEVAKSMAKASESKVLSLQSTYGICESMDGQFMPTKYQAKDDSVGEM
                     MLEMTSRIYNYKYINEVKKYYHDNAVVHFICDKDLNGYDEIQGMIVSLLASIPNGSYE
                     VERVTCNQREKNEGYDVSVRWRLRGINEGIGFFGQPSGKHIEVMGINHYHILQGKVKE
                     EWITFDGMDVLKQMYMGVEE"
     gene            7750..9015
                     /locus_tag="KPD58_002040"
     CDS             7750..9015
                     /locus_tag="KPD58_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0265806.1"
                     /translation="MKTTVEIENDIILGKDYSNLKRGIAFFSIALIYFFYCYNFMVGT
                     FIKPTMIYALADGGFGFSLKQTEEIFAVMSFGTIPGTFIFGVISTKIGKKRTLISVAL
                     LIGLTTFIPMLSPTNIMLWKIARLCTGVVLGGVFGTAMPLVADMFPSKYRGKLAAILT
                     ALFSLAMIFGGKVYGVLGDANWQILMYTAIIPPIVGAILAIFFVPDDLEYTKELVKKG
                     KETGEKISYISMYKGKYLWIGLGTILLSGANFTAYSAFSNNATTYLVTGIGMSAAVAG
                     SIYSLQGIGQLVGYLFWGSIADKFGRKIPFIGMALAAVFVFIYTKLGGDNTTTFYMIS
                     VLLGVAVGYSGAWGAYYTELFPSKYRSLSSGMSFNGGRIISTFAIPAIAGTASGTLGM
                     MTIFYISIAVFVAGAIVWLFLPETLNNKK"
     gene            9057..10655
                     /locus_tag="KPD58_002041"
     CDS             9057..10655
                     /locus_tag="KPD58_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HBG0265807.1"
                     /translation="MISSIDVCIIIGYLLLMLVIGYYSGKDNKNQEDYFLAGRSMPWI
                     PIALSVAATMISANGFIGGPGWAYVDGMYPVMVNITVPLAIFFALSITTPVIYKLKIT
                     SIYEYMGLRLGNYSRGLTIIQFFINSLIQASSMVYIPVLIIQMITGWSLYALVPIVVL
                     VSIIYTLLGGIKAVIWTDSIQMIVVISAVFIVIIVALKGMNLSFFDTLQIAQQSGKLN
                     TFDFSRDISVTNTFWATLIGGTVMWIRYFCFDQSQVQRVLTSKSLKCAKNSFVVSAFV
                     MNLVYYFMLLVGVILFVFYGGRTFETSNEIMITFILNELPVGAIGIIIAGVFAAAMSS
                     IDSILNSMTTVFTKDIYEFYFKKDNKESSLKVTMIITVVIGVVMTGFIIMGFGGSIKS
                     ILDLVGNYISYFAGPATGAFVLAMFTYKANDKGVSIGFIVGLVLGFFISNKYNVSWLW
                     NPAIGATITLIFGYIFSVILKKNHNADDIKKYTAFGMRNHLISQGMDKVDGVSIVPFS
                     LGKQELIVLAFFIIQYIILFLIQF"
     gene            10690..11712
                     /locus_tag="KPD58_002042"
     CDS             10690..11712
                     /locus_tag="KPD58_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG0265808.1"
                     /translation="MNQKKVHYNKSATSKDVARLAGVSQSSVSRAFGSANGKGVKPEV
                     RERIFRVANEIGYVPNLVARGMISGKTNVIGLIVGDSLGPFYNRIINLFVEKIQEIGK
                     QCLVFKVPRQQSIDSIISKVIQFQVEAVIITASAMNKVMAETCEENNIPVILFNRFIP
                     GIKISTVYVDPIEGGGMAAEYLLKKGHKNIGYIQFTRETSEEMEKKIGFYSKLRQSGI
                     HNLKEESANYDYDSGYEAGKRMLALSNPPTAIFCTSDLIAIGVMDAARFEYKLRVPED
                     LSVIGYDDIQMASWKSYNLTTIRQPLDLLIEKTINILKSLLNEENPESVVEMLKPELI
                     ERGSTL"
     gene            complement(12189..13592)
                     /locus_tag="KPD58_002043"
     CDS             complement(12189..13592)
                     /locus_tag="KPD58_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0265809.1"
                     /translation="MKMLNNFFKKINNPAPVFAQTKMERDNQTDDSLSTKPKTTFRDV
                     AGLDEVKEELFEIVDFMKSPQKYQKMGAKIPKGVLFYGPPGTGKTLLASAVAGETNSS
                     FFNVTGSEFVEKYVGVGAKRVRTLFEKARKEAPSIIFIDEIDAVGAKRHLESNNEKDQ
                     TLNQLLVEMDGFNKDSNVLIIGATNRLDLLDEALLRPGRFDRHIHIGAPNYHTRFEIL
                     KVHTDDKPIDKSVNLELLAKKTHGFNGAHLSNIANEAAIFAVRDDSECITSEHFDKAL
                     ERVIAGLESKNSALVEKEKKIVAYHEAGHALVSDIVGICPIQKISIVPRGQALGYVLQ
                     LPDEDRYIYTKDELIGKIKILLAGKASEELIFNHKSTGAKDDLKKVTEIANQMVCEYG
                     MSNLGFMTIDGNDKTFLCDKVQKEANRIVEICYKETLEMLKDNLEDLHSVSKFLFEKE
                     TMTHEELKDLIGKEAVN"
BASE COUNT         4917 a         1668 c         2633 g         4733 t
ORIGIN      
        1 tgagacttta agacagataa tctcctatta aagatactat atcttagata aaataaatta
       61 aaacaatata aaaacataac aagattacag aaagaaagct cacaataaaa tgctgaaaaa
      121 tttattttaa ggaggaataa agtatgaagt ttcaagaaat gattaatagc ccaggtcttt
      181 ggattgtttc tagtttttta gtaattattt cagttgttca agcaatcgta tttatgaaag
      241 aggcactaaa agaggcttca aatcttggta ttgaaaggaa aggaattaaa gcagcaattc
      301 gctcagctag tgttacagcc attggacctt ctctatctcc tgttattaca ttattatcac
      361 tggtagctgt gattggcgct cctacgactt ggatgaggct ctgtgatgtc ggagcagcta
      421 gaacagagct tggagtaata tctcttacat ccaatttatc tggggtagaa gtaggtagtg
      481 ctgcatttgg agcagaagct ttttcgtatg cactatgggg gatggcttta aataacttag
      541 gttggctatt tgttgtattt attttgggtc atagaatgcg tggaattgtt gaaaagatga
      601 acatgaagta taatcctact tgggttaaaa aactacttgc aggagctaca gttggattat
      661 ttgcttacct tctttcaaat caaataaaaa catttgaaat accaaaactt acaccagcaa
      721 ttatttcagc tatagttatg ctagtattga caactttatt taaaaagaat caacgattac
      781 aagaattatc cttaggtatt gccatgttaa ttggtatgtt tggtacacag attatattat
      841 tgtgattaaa acggataaga ggtgataatt atgtatgata aaaagatgat aaaaataggg
      901 agaatttcta taggtcttgc aattattgca aactttattc ccgctatcta tgtaggtatt
      961 cgctttggtc agatgccaat gctttctgtt atatttcaaa tttgggggtt agtcgctgcg
     1021 acttatgggg ttagttggat tgttcaacct atagcttatt atcctacact aggagctcca
     1081 ggttcttata taggttggct tgcaggttct gttggagata tacgtatgcc tgcagcatca
     1141 atggcacaga aaattgcagg tgtagaagca ggaactcatg agggtgaagt tgttggaaca
     1201 attggtacat gttgttcaat ctttgtatca gcttctatga ttaccatttt taccattgta
     1261 ggctataaag taattccttt gttacctgaa tttgttacta attcatttaa ttatatactt
     1321 ccagcattat ttggagcaat ttatgttgat attgcacgta aggatattcg agctggtgga
     1381 tgtacgattg ctgtagcgtt agcaattatg tactttggtg aaaaaattgg tataccaggt
     1441 ggattattaa cattgtttat tgtgttaggt gggatactta tcacaagggt cttctttgta
     1501 attgataaca ataaaacaaa agaataggat tgcttattaa ggagaaagac tagaattatt
     1561 tgttgagaaa aaagaatagg attgtttatt aagaaatatg aatataaata agtcttgaaa
     1621 ggagaaaaag catgtttgat gttaaaattg tcaatggaat aatcgttgat ggtacaggaa
     1681 attcaagata taaaagtgat gtagggattg taggagacaa aatcatggct attggagatt
     1741 taagtcaaaa agaggctaaa gagactatag acgcaactgg aaaatttgta tcacctggtt
     1801 ttatagattt ccatactcat tctgatttat ctcttgtgta tgataaatac acaagaagcc
     1861 gtatccatac aggtgtaaca acagatgtta ttgcaaactg tggtatagga gttgctccga
     1921 taagagaaga gaagaagcaa gagttaattg attatcttgg aactaggata attggtacaa
     1981 ttccaacaaa attagagctt cattggaata caatgcaaga atactttgat tacttaactg
     2041 aaaattctcc agcagtcaat gttgtagctt atgtagcaca aggacctgta cgtatcaatg
     2101 aaatgggatt ttcaaaggaa ccagctactt tagagcaact taaaaatatg aagttggaag
     2161 ttagaaaagc tatggaagct ggatgtgtag ctctttcatc aggattagtt tatttaccag
     2221 gagcatacac aaagaaagaa gaaatggctg aattatgtaa ggaattgata ccatacaatg
     2281 gatactatat atctcatatt agagatgaag gtgatgaaga aatggaagct cttgatgaag
     2341 caatttatat agctaagact gcaggagttc ctctacatgt ttctcattta aaagttatgg
     2401 gtcataagaa ttttggaact attgatgagg tatttaaaaa gctagatgaa gctgaggcag
     2461 atggtttgga agtaactttt gattgttacc catatacagc tggaatgaca tctttgggtg
     2521 cacttttacc tccttgggct tttgaaggcg gtgtagaaaa tatggtaaaa cgattagaag
     2581 tacaagaaaa tagagacaga attataaaag agttggagga agggattcct ggatggcaat
     2641 gtttctacca attagcaggt gggtggaacg gtgttgtact agcctctgta atgactgaag
     2701 caaataaata tgtagagggt aaaactttaa tggaggtagc caaaatcaat ggtgaaaatc
     2761 catttgatac gttctttagg cttttgattg aagaaaaaag taaaattcaa gtagttgttc
     2821 atacacaagg tcaagaggat acagataagg ttgtttgcca tccaaagtca tgtataggtt
     2881 ctgatagtat ggacctttct acagaaggtt tactttctct tggaaagcct catcctcgtg
     2941 catttggtac ttttggacga attttctctt actatgttag agaaaaaggt atgttgactt
     3001 ttgaggaagc agttaaaaag ataacttatc tttcagcaaa acgcttaggc atttataaag
     3061 aaaggggtct acttaaagaa aattattttg ctgacattgt ggtatttgac ccagatacaa
     3121 tagaagataa agcgacttat agtaatccta aacagtatac agtgggagtg gaatatgtgt
     3181 tggtgaatgg taaaattgca ttggcaggag gaaagcaaac tgatgtatgt gctggacgtg
     3241 taattaaaaa tcctcttagc atagcaaaat agaatacatt aagaactttt ataaaaatag
     3301 tctttctttg aatattaagg aaggctattt ttgttaaatt aataaagatt actctacttg
     3361 tgttttctaa agaatttaac tttaagtaga aaaaacaata tattaattgt aaaatgtaat
     3421 taatattaat gaataagatt taaatacaaa aatttaaata caaaaatgtt tcaacagata
     3481 gtcgtaatta aaaaacttaa agtaactttt tatattaggt ataattgatt attcaaaatt
     3541 ctatgaatca ggagtcctgt ggctttagtc atggtaggtt tagaaaatgt tttaagagat
     3601 aatagtatca ttaggaaagg agagacgaaa tgaaaatact ttatgtagga ccaggtatgg
     3661 taatcgataa aatactagca gtaagtaaaa agaattttcc taatattgaa gcagattttc
     3721 ttagatatga tagatatatt gaagtacctg atttattgga aaaatataga ggtagagtgg
     3781 atgcagttct ttttacaggt aagtcgccat ttaaattttt tgaaaaaaga caaaaagatt
     3841 ttgtattgtc tgattatatt cctagacatg aaacaacatt gtacagagtg ttgcttgaaa
     3901 ttacatattt gcttaaatta gatattagta gacttagtat agatacttat gataaggcta
     3961 tgatgaagag attgtataaa gaaattggag ttgaatttaa ggatgaacat atgttttttg
     4021 cagagcaaaa gtatttagaa gaggactata taaagtttgt gcttgaattt cataaatata
     4081 attatcaaaa taatgatgta tgttgttgta ttacaggtct tgaagaggcc tatcatataa
     4141 tgaaaaaaga taatattcca gtagttttag ccttgccttc tgaggacgta atggtgcaaa
     4201 gtataaaaaa cattcagatg cgttatattg caaagaaaaa caatgagaat cagattgttg
     4261 ttcttgctat taaactgaac atgccttcag aatattcact aatgaaggaa gatgagtacg
     4321 cttatctttc acaaaggatt aagattcttg ataaactata ccatttcaat agccgtatag
     4381 atggtgtttt ggtagagcag agtagaagtg aatttatgat ttttacaact aaaaaaatac
     4441 ttgagttaga aacaaagaaa tacagagata tttatttgtt ggatatgatt cgtgaagttt
     4501 cagttataaa cacttatata ggaattggtt atggaaaaac agcaaatgaa gctaaattta
     4561 acgcttatga gagtattaag atggctcaaa gtcaaaaaaa tgatgcagca tatgtagttt
     4621 ttgaaaatgg agaaattatg ggaccactaa agccaaatcc tactgtcaaa gagaaaaata
     4681 gttttgatga acgtttttat caaattgcta cagagacagg gctaagtgct aatactgtat
     4741 ataaagtatt caataaaata ataaatagtg gaaaaactga atttacatca aaggaattag
     4801 ctactatatg tggtgtaagt attagaacta tggacaggat tatacttaga ttgtgtgatt
     4861 ctggttattg tgaggtagtg agtgaacagc ttatgagcaa gtatggtaga cccagtagaa
     4921 tattacgttt taaacctttt ctgttgtttt aaatagatat aagtcaaaaa ttaaattttt
     4981 atgataacat tttctttaca attttttaga tatatgttaa tatttagaaa aatgcatatg
     5041 tattcataaa ataaacttaa aacagctgaa aaatcattaa aaaggaaatc gtatgcatga
     5101 aatgagaaag ttaatttata acttaaataa atctgggagg agataattat gggaaaatat
     5161 cagtattcaa aaaaagttgt aagaaattat tttgaagcat tagaaaatgc tactggggat
     5221 gaggttgaaa atgtcttaaa gcaacatatg aaatcagatt ataactggaa aggtgtgtat
     5281 ccatttcgtg aacaagaagg cactgaaaat gtagcagatg tattttggaa gcctttaaaa
     5341 aaatcattga gtaatatgca aagacgacaa gatattttca ttgcaggaac aaatgagatt
     5401 gacaatagtg aatgggtaat gagtatgggg cattttatgg gactttttga taacgattgg
     5461 cttggcatta aacatactcg taaaatgatt agtttaagat atgcagaatt taactgtgtt
     5521 caagatggta aaattgctaa aactggatta tttgtagata taattgggtt tatgattcaa
     5581 gcaggagtta atccattgcc accacaaaca ggttcatatt ttgtatatcc agggccaatt
     5641 gaccataatg gattactttt tgaagatgca gatgaagttg aaggagtaaa aactttagca
     5701 ttagtaaata aaatggtaga tgatttatct gaattaaatg aaagtggtgc tatgggatgc
     5761 ccaccagaaa tacttgaaaa atcttgggct aaagatatga tttggtatgg tccaggtgga
     5821 attggtgctt cttatactat acctagatat caagagcaac accagctacc atttagaaat
     5881 aatttaaaag gaaagacttt caatgggcat gtatgtagat ttgcagaagg taattttgct
     5941 tgtttctttg gatggcctaa tctgactaat gtaccatgtg gaggtttttt aggattacca
     6001 ggtggagagg ttaaagcaga tatgcaagtt gttgatgttt attatcgtaa gggcgataaa
     6061 cttcaagaaa actgggtgtt aattgatatt ccttattggt tgaaacaaca agggcttgat
     6121 attcttgaga gaactaaaag tatttttaat gcttagttat gcatacgtat tctaaaacaa
     6181 agggggatat aaagtggcat ctaccaacaa aaataatgca acaagtaaag aggaagaaaa
     6241 agaagtttct acaagtgtgc ttgaatcatg tacggttaaa gaaaaaaaag ctaaaatgaa
     6301 tcaaactaat tttagtaata taaatgaaaa tcaaacaaat gagaataaaa cgaacacata
     6361 taaaggaaat atagataaag gaaatataga taaaacaaac acaaataaat ccgacataaa
     6421 taaagttaac atagatacag tggatacaga taaattaaat acaaatactc ttactgaaaa
     6481 aaataaaaaa gttatattta agggggaaga taatatgtca tctacaaaaa tttactatgg
     6541 agattcatta gaagtaacgc ctgttggagt aatggattac aatgattttt caaaacagac
     6601 aaaaagggaa caggaaatac ctggatttga ttctaagtat agagattttg tagattatat
     6661 tatgaagatt acacataata tctgggaaga aaaaggtata ggtgttattt atgatacata
     6721 tcacaataat gttacaatgc attgtggctc ttcaaattta gttggtatta aagatgttat
     6781 ttccaatact cttcaaacat tacatgcgtt tcctgataga agattaattg gtcaaaatgt
     6841 aatatggtct aactttggtg ctgatggttt tttatcttct caccgtgttt tgtctacagc
     6901 aactaactta ggtgatagta attttggacc agcaacaggt agaaaaataa actttagaac
     6961 tgtaattgat tgtgccacaa ctaacaatag aatccacgaa gaatggttgg ttagagataa
     7021 tttatggatt gttactcaat taggattaaa ccctcaggaa gtagcaaaga gtatggcaaa
     7081 ggcaagtgaa tctaaagtgc ttagtttaca atcaacttat ggtatctgtg aatctatgga
     7141 tggtcaattt atgccaacta agtatcaagc aaaagatgat tctgttggcg aaatgatgct
     7201 tgaaatgaca agccgtattt ataattataa atatataaat gaagttaaaa aatactatca
     7261 tgataatgca gtagtacatt ttatatgtga taaagattta aatggctatg atgagattca
     7321 aggaatgatt gtaagtttac ttgcatcaat tccaaatgga agttatgaag tggaaagagt
     7381 aacttgtaat caaagagaaa aaaatgaagg ttatgatgta tctgtaagat ggagattacg
     7441 tggaataaat gaaggtatag gattttttgg acagccttct ggaaagcata tagaagtaat
     7501 gggaatcaat cattatcata tactacaagg taaagtaaag gaggagtgga taacttttga
     7561 tggtatggat gtattaaaac aaatgtatat gggagtagaa gaataaagct gtttagagta
     7621 taaatagttt tgaaatgtat atagggaagt attgctttat aaaatttaag tggggaatgt
     7681 atatgaaaaa tagatgttta aacttttagc aaaattatga ctaaatttaa aagggggaat
     7741 tataaaaata tgaaaacaac ggttgaaata gaaaatgata tcatattagg aaaagactat
     7801 tcaaacctaa aaagaggaat tgcatttttt tcaattgctt taatatactt tttttattgc
     7861 tacaatttta tggtgggtac ttttattaaa ccaacaatga tttatgcttt agcagatggg
     7921 ggatttggtt tttcattaaa acaaacagaa gaaatatttg cagttatgtc ttttggtaca
     7981 attccaggta catttatttt tggggtgatt tctacaaaaa ttgggaagaa gaggacttta
     8041 attagcgttg ctttactgat tggattgaca acatttatac caatgttatc tcctaccaat
     8101 attatgttat ggaaaatagc acgtttgtgt acaggagtag tgttgggagg cgtatttgga
     8161 acagctatgc ctttagttgc agatatgttt ccaagtaaat atcgtggtaa gttagctgct
     8221 atattaactg ctttattttc attagcaatg atttttggtg ggaaagtata tggcgtttta
     8281 ggtgatgcaa attggcaaat attgatgtat actgcgatta taccacctat agttggagct
     8341 atcttagcaa tcttctttgt gccagatgat ttggagtata caaaggaatt agttaaaaag
     8401 ggaaaagaaa ctggtgaaaa gattagctat ataagtatgt ataagggaaa atacttatgg
     8461 attggattgg gaacgattct tttatcaggt gcaaatttta cagcatattc agcattttca
     8521 aataatgcaa ctacatacct agtgacagga ataggaatgt cagcagcagt ggctgggtct
     8581 atatatagtt tacaaggaat tggtcaatta gtaggatatt tattctgggg ttcaattgca
     8641 gataaatttg gaagaaaaat accttttata ggtatggcat tagcagcagt atttgttttt
     8701 atatatacaa aattaggtgg agataataca acaacattct atatgatatc agtattacta
     8761 ggtgttgctg ttggatattc tggagcatgg ggagcatact acacagagtt gttcccaagt
     8821 aaatatcgtt cgttgtcatc tggtatgtca tttaatggag gaaggattat atctacgttt
     8881 gcaatacctg ctatagctgg tacagcttct ggtactttag gtatgatgac tatcttttac
     8941 atatcaatcg cagtctttgt agcaggtgct attgtatggt tattcttacc ggagacgtta
     9001 aataataaaa aataaaatac taggaattta ttttgttact tagaaagggg tggcttatga
     9061 tttcatcaat tgatgtatgt attataatag gatatttgct attaatgtta gttattggtt
     9121 attattcagg aaaagataat aaaaatcaag aagattattt cttggcaggt aggtcaatgc
     9181 catggattcc aattgcttta tcagttgcag ctactatgat tagtgcaaat ggctttatag
     9241 gtggtcctgg atgggcatat gtagatggaa tgtatccagt aatggttaat attacagtac
     9301 ctttagcaat attttttgca ctgagcatta ccaccccagt tatctataaa ttaaaaataa
     9361 cctctatata tgaatatatg ggacttcgtt taggaaatta ttcaagagga ttaactatta
     9421 ttcaattttt tattaattca ttgattcaag ctagttctat ggtatatatt ccagtgctta
     9481 ttattcaaat gattactggc tggtcacttt atgctttagt cccaatagtt gtacttgtat
     9541 caattatata cactctatta ggtgggatta aggcagttat atggactgat agtattcaaa
     9601 tgatagtagt aataagtgct gtatttatag taattattgt agccttaaaa ggaatgaatc
     9661 taagtttctt tgatacatta caaattgcac aacagagtgg aaaattaaat acatttgatt
     9721 tcagtaggga tatttcagtg acaaatactt tttgggctac tttaattggt ggaactgtaa
     9781 tgtggataag atacttttgt tttgaccaat ctcaagtaca aagagtttta acatctaaat
     9841 ctttaaaatg tgcaaaaaat tcttttgtag ttagtgcatt tgttatgaat ttagtttact
     9901 actttatgct attggtagga gtaattttat ttgtttttta tgggggaagg acatttgaaa
     9961 cttcaaatga aataatgatt acttttattt taaatgaact tccagtagga gccattggaa
    10021 ttattattgc aggtgtgttt gcagcagcaa tgtctagtat cgactctata ttaaacagta
    10081 tgacaactgt ttttacgaag gatatttatg agttttattt caaaaaagat aacaaagaat
    10141 catcattaaa agttacaatg attataactg tagttatagg agttgttatg acaggtttta
    10201 ttattatggg atttggagga agcatcaaat caattcttga ccttgttggt aactatatct
    10261 catactttgc tggaccagct acaggtgcat ttgtactagc aatgtttaca tataaagcta
    10321 atgataaagg agtatctatt ggttttattg tagggttagt tttaggattt tttatttcta
    10381 ataaatataa tgtgagttgg ttgtggaatc cagcaatagg tgctacaata acattgatat
    10441 ttggatatat ttttagtgtc attttaaaga aaaatcataa tgcagatgat attaaaaaat
    10501 acactgcatt tggtatgaga aatcacctta tttctcaagg aatggataaa gtagatggag
    10561 tatctatagt gccattttct ttaggtaaac aagaattaat tgtattagct ttttttatta
    10621 tccaatatat tatattattt ttgatacaat tttaagaaga aatactagat atttaaggag
    10681 aaatactaaa tgaatcaaaa gaaagttcat tataataaat cggctacctc aaaagatgtt
    10741 gcaagattgg caggagtatc tcaatcatct gtttcaagag catttggtag tgcaaatgga
    10801 aagggcgtaa aacctgaagt aagagaaaga atttttcgtg tagcaaatga aataggatat
    10861 gttcctaatt tggtagcacg gggaatgatt agtggaaaaa ctaatgttat aggattgata
    10921 gttggtgata gtctaggtcc attttacaat agaattatca atttatttgt agaaaaaatt
    10981 caagagatag gtaaacagtg tctcgttttt aaagttccta gacaacaaag tatagatagt
    11041 ataatttcaa aagtaattca gtttcaagta gaagctgtga ttattacagc ttcggccatg
    11101 aataaggtga tggctgagac ttgtgaagaa aacaatattc ctgtgattct ttttaatcgt
    11161 tttatacctg gtataaaaat aagtacagtc tatgtagacc caattgaagg tgggggaatg
    11221 gctgctgaat atctgcttaa gaaaggtcat aagaatatag gatatattca atttacgaga
    11281 gaaacaagtg aagaaatgga aaagaaaatt ggattttact ctaagttaag acaaagtggg
    11341 attcataacc ttaaagaaga atctgcaaac tatgattatg attctggata tgaagcaggt
    11401 aagagaatgt tagcattatc aaatcctcca acagcaattt tttgcacaag tgacttgata
    11461 gctattggtg taatggatgc tgcaaggttt gagtacaagt tgagggtgcc agaggattta
    11521 tcagtgatag gatatgatga tattcaaatg gcttcatgga agagttataa tttaacgacg
    11581 attagacagc cactagattt gttgattgaa aaaacaatta acatacttaa gtccttatta
    11641 aatgaagaaa atccagagtc agtcgttgaa atgttaaaac ctgaattgat tgaaagaggt
    11701 tctacacttt aagtggtaga tatttaatta agtggtagat atttaattaa atggcagata
    11761 tttaatcaag tgatagatac ctaattaagt gatagatatt taatcaagtg atagatacct
    11821 aattaagttg tagatatctg ataagaaatt tttaagtttt caatcgttct aaattgtgag
    11881 tataaaaatc tactaaattt agttatgtaa aatataaatt ataagtgtag aaactaggct
    11941 actagctttt ataccacttc aaacattact ggaggtagtt aagtttacag cagtttagtt
    12001 ttatgtagat ataatattct gtaaatagac aaaattaaaa tatgtaatta aaatatgtaa
    12061 gtgtctcaaa atgaaatttt tagtttatga ggcacttttt tgtataaaat caaatgtatt
    12121 tttataactt taacaataaa aagaggctta tctctatttt gagacagcct caaattttta
    12181 aaactatatt agtttacagc ttcttttcct atcaaatctt ttaactcttc atgagtcata
    12241 gtttcttttt caaataagaa tttagaaaca gaatgtaaat cttcaagatt atcctttaac
    12301 atctctaaag tctctttata acaaatttca actatacgat ttgcttcctt ttgaacctta
    12361 tcacataaga aagtcttatc atttccatca atagtcataa atcctaagtt actcatacca
    12421 tattcacaaa ccatttggtt agctatttca gtgactttct ttaagtcgtc ctttgcacca
    12481 gtagatttat gattaaatat taattcttct gaagcctttc cagcaagtaa tatttttatt
    12541 tttccaatca actcatcctt agtataaata tatctatctt catcaggtaa ttgaagaaca
    12601 taacctaatg cttgtcctct aggaactata gatatttttt gtataggaca aattcctact
    12661 atatcactta caagagcatg tccagcttca tgatatgcta ctattttttt ctctttttca
    12721 accaatgcag agtttttaga ttctagccct gcaataactc tttcaagtgc cttgtcaaaa
    12781 tgttctgatg taatacactc actgtcatct ctaactgcaa atatagctgc ttcatttgct
    12841 atatttgaaa gatgagcccc attgaaacca tgagtttttt tagctaaaag ttctagattt
    12901 acagatttgt ctataggttt gtcatctgta tgtaccttta gaatttcaaa tctagtgtga
    12961 tagtttggtg cacctatatg aatgtgtctg tcaaatcttc ctggtctaag taaagcttca
    13021 tctaataagt caagtctatt ggtagcacct attattaata cattggaatc tttattaaat
    13081 ccatccattt caactaaaag ttggtttagt gtttggtctt tttcgttgtt actttcaaga
    13141 tgtctctttg ctcctacagc atctatttcg tctataaata ttatacttgg agcttctttt
    13201 ctagcttttt caaatagagt tctaactctt tttgctccca cacctacata tttttcaacg
    13261 aactctgaac cagtaacatt gaaaaatgat gagtttgttt caccagctac agcagatgca
    13321 agtagggttt tacctgttcc tggaggacca taaaataata ctcctttagg tatttttgca
    13381 cccatttttt gatatttttg tggtgacttc ataaaatcga ctatttcaaa tagttcttct
    13441 tttacttcgt ctaatccagc aacgtctcta aatgtagtct ttggttttgt agaaagtgaa
    13501 tcatctgttt gattatctct ttccattttt gtctgtgcaa agacaggagc tggattgttg
    13561 attttcttaa aaaagttgtt tagcattttc atattcacac ctcaggtaat taattattgt
    13621 taatcaattt tatattttcc atatttacta ttttttatta tttatttttc atggaaaaaa
    13681 tatacatgtt atctaaataa aaataaattt gtaagatgta tgaaaaaatg aatttatgta
    13741 tatagagatt tctacttatg tattgaataa agtattacaa attataaatt atgtattgat
    13801 tatatattga gtaaagtatt ataaagcatg tattgattaa gtatttatta agtattgaat
    13861 aaaatattac aaatttagtt gaaatgaaaa tgataaaaaa tgttgtacta tattttacaa
    13921 atcatataaa tagagaaatt gttgtagcgc a
//
LOCUS       DAELLV010000090        13834 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.090, whole genome shotgun sequence.
ACCESSION   DAELLV010000090 DAELLV010000000
VERSION     DAELLV010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13834
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.090"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            120..1034
                     /gene="murB"
                     /locus_tag="KPD58_002044"
     CDS             120..1034
                     /gene="murB"
                     /locus_tag="KPD58_002044"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBG0265810.1"
                     /translation="MNNQDIYINLLNILSKEDIKVDEPMKKHISFRVGGPADILVRPR
                     TEEQLKNVLKLVKEESIPYLIIGNGSNILIKDGGIRGVVIELADNFNSYEINDTRMTA
                     QSGALLSVLGKALQKQELKGFEFASGIPGTLGGALAMNAGAYGGEMKDIVKSVRLMDM
                     EGNIFELSNEQMEFGYRKSIISKNGYIALSAELELQEGNYDEIKSLMDDLATRRITKQ
                     PLNFASAGSTFKRPTGYFAGKLIEETGLRGLTLRGAQVSEKHCGFVVNQGEASAKDIL
                     DLIYVIKSAVYAKFGVMLEEEVKILGED"
     gene            1038..1751
                     /locus_tag="KPD58_002045"
     CDS             1038..1751
                     /locus_tag="KPD58_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBG0265811.1"
                     /translation="MKILADYHTHTLYSHGKGTIEDNVKVAISKGIKTIGISDHSYKH
                     IAYGVKKKDILKMREEVDYLNDKYTDINILLGMECNILDDKGNIDMDDKFIRDFDYIM
                     AGYHFGSTPTSLRSLLNHCNNYIFKTEKAKEYNTKAVINAISNNDIFIITHPGDKGDV
                     YIEEVAKTAKKTNTRLEINSSHSFLNVNQLNKIKGLENKFIIGSDAHCPERVGDFELA
                     MKTISDSNLDISLVENIII"
     gene            1782..2639
                     /gene="rapZ"
                     /locus_tag="KPD58_002046"
     CDS             1782..2639
                     /gene="rapZ"
                     /locus_tag="KPD58_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBG0265812.1"
                     /translation="MKFVIVTGLSGSGKSETMRALEDMGFYCVDNLPPALITKFAELC
                     YQPNSSIDKVALGIDIRGRKFFEALHESLNYLEKENYEYEMVYLDCNDDVLLKRYKMT
                     RRNHPLAKDMQIPEGIKMERKIMEPLKELSTCIIDTTNMKPKDLKEEIKKIYSSGEDN
                     PNLTISVVSFGFKHGILADADLVFDVRFLPNPYYVEELRAKTGDDKEVRDYVMNSKIS
                     EEFYVKLLDMIHFLVPQYIEEGKQHLVIGVGCTGGRHRSVTITNLIAEDLSNKGYRVV
                     KKHRDSMLR"
     gene            2651..3757
                     /locus_tag="KPD58_002047"
     CDS             2651..3757
                     /locus_tag="KPD58_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HBG0265813.1"
                     /translation="MAKLLIIVGILGWIALIVSLFIYKKAKNEKVKLFQRSIVENREP
                     NVVVIGGGTGQSVFLRGLKHTTQNITAIVTVADDGGGSGVLREDLGMLPPGDIRNCLL
                     ALANIEPTMNEVMQYRFTEGLLKGQSFGNLFLAAMNGLYGNFEKAVYKLSEIFAITGR
                     VLPVTLEDVNLVAKLENGNIINGESSIPEESKIQKSSIDKIFLNPKDVKPLKDVIASI
                     YDADIIIMGPGSLYTSIIPNLLVEGIVDAIKSSVAPKVYIANIMTQPGETEGYNVLEH
                     VNAIVKHTDENLIDYVIANNEILPEEMLDLYKQDGAEQVLLDKKQKDKLKEMGIKTVE
                     KNLIEIKNNYIRHDAKYISNIVIELALNHNYNKS"
     gene            3826..4251
                     /locus_tag="KPD58_002048"
     CDS             3826..4251
                     /locus_tag="KPD58_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG0265814.1"
                     /translation="MREEVSAGGVVLFGNTILLLRKFNGDWVLPKGKVEEGENNQEAA
                     LREVSEETGVKADILKYLGEIHYTFKENWDENRAVHKTVFWYLMQAKNMDTIPQKEEG
                     FIDAKFIHLDRVVDLARYDDEKEIIKVALQEIKKRLKKN"
     gene            4268..5215
                     /gene="whiA"
                     /locus_tag="KPD58_002049"
     CDS             4268..5215
                     /gene="whiA"
                     /locus_tag="KPD58_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HBG0265815.1"
                     /translation="MSFSTETKNELARVISGNECCNIAELSALVKSGGSIQIVGYKKL
                     NLKITTELNSIARKVFKLLKKNFNINTTISVNKNQMLKRNNSYVLMVTSEMGSEMLLK
                     KLGILEDKEGFFTINKISENLIKHEGCKRAFIRGAFLGGGSISDPEKNYHMEFVTNNE
                     EFADSLKELINSLGFNSKIVARKNNHVVYIKESEQISDLLSIIGGHHALLSLQNTKIV
                     KEMRNNVNRIVNCETANLSKTVNAAVRQVENIKLIQETIGISSLPENLQEIAKIRIEY
                     EDMTLKELGEMLEPPIGKSGVNHRLRKIEEIATDLKKKS"
     gene            5372..8941
                     /locus_tag="KPD58_002050"
     CDS             5372..8941
                     /locus_tag="KPD58_002050"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBG0265816.1"
                     /translation="MNKDFAHLHVHTEYSLLDGFSRVKKLIKRAKELNMSSIAITDHG
                     CMFGVIDFYKTAIKEGIKPIIGCEVYTAARGLRDKDPNYDKYQGHLVLLAKNMEGYKN
                     LIKIVSTSYVEGFYYKPRVDMEELKKHNKGIIALSACLAGDVARALMNRNYEKAKQFA
                     LDYRDIFGEENFFLEIQDHNLPEQKEVNSGLVKLSKETGIPLVATNDVHYVNKEDSKI
                     HDVLMCIQMGKTLNDPNRMRFGSDEFYLKSREEMEELFPYALEAIDNTVKIADMCNVE
                     FDFNTIHLPKYDVPEGYTPETYLRELCFNGLKERYDNPSNEILERLNYELDVIEKMGY
                     VEYFLIVWDFINFSKENNIIVGPGRGSAAGSIVAYTLKITDIDPIKYSLLFERFLNPE
                     RISMPDIDIDFCYERREEVIDYVKRKYGDDHVAQIITFGTMGAKAAIRDVGRVLDIGY
                     NKVDKVAKEIPFALGMTIDKALDTNPNLKELYDQDPETKEIINISKQIEGMLRHASTH
                     AAGVVISKNPVDEYVPLYKHQDAITTQFTMTTLEELGLLKMDFLGLRTLTVIRDTLDL
                     IEKNRVIKNYTEKIDFSKMDYDDPKVYEMLSSGNTLGVFQLESAGMRSFMKQLKPDNF
                     EDIVAGISLFRPGPMDSIPAYIKNKSNPKDVTYLHEKLKPIMEVTYGCLVYQEQVMQV
                     VRDLGGYSYGRSDLVRRAMSKKKMDVMEEERQYFIHGKFDEDGNIEIAGCIRNGVEEE
                     IANKIFDDMIDFARYAFNKSHAAAYGVLAYETAYLKTYYPVEFMAALITSVMGNTDKV
                     VEYIRECKALEIDVLPPDINKSFSKFSVEGDNIRFGLAAVKNVGVNIINNIIAERESN
                     GLFVDLVDLVKRLDQKDTNKRVIESLIKCGAFDNISENRASLMAGYESLLESVSMDRK
                     KNVQGQISLFDAFGDSEEDSDFQQIYSLPKRQEYEERERLNLEKEVLGMYVSGHPLSQ
                     FEKELQEKTSMDNGKLNSLKEEEESYLQMNETDVIMGGMIVTKTIKTTKRNEIMAFIE
                     LEDLYGAIEIIVFPQLLQKYNVILNEDNIIYVKGTLSIKEGENAKLIAREIKDINDDT
                     DFADRNYNSGRTRNVENIKPESGKKLYLKIDSMSDTDTTNNIVEIAKQYPGNDSIYLY
                     PMDENINGKRKAYKMTGALTCICDEFINNLEELLPKDNIKVKA"
     gene            9150..10109
                     /gene="pfkA"
                     /locus_tag="KPD58_002051"
     CDS             9150..10109
                     /gene="pfkA"
                     /locus_tag="KPD58_002051"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBG0265817.1"
                     /translation="MKTIGLLTSGGDAPGMNAAIRAVVRSAIYYGCKVYGINRGYKGL
                     LEEDLTEMNLSSVGDIIHRGGTILKSSRCEEFKTEEGRLKAVKILKKYKIDCLVVIGG
                     DGSFAGAQKLSDLGFPAIGIPGTIDNDLAYTDYTIGFDTAMNTIIDAIGKIRDTSSSH
                     ERVNIVEVMGRHCGDLALYAGLAGGAETIIVPEVEITVDEVALRLKTTQKRGKRHSII
                     VLAEGVGSASDLEKELKKESGADLRVTVLGHVQRGGSPTVSDRILASRLGVRAVELLL
                     DGKSARVVGIKENKIIDLEISEALAQKKVFDKEAYEMAKILSI"
     gene            10161..11921
                     /gene="pyk"
                     /locus_tag="KPD58_002052"
     CDS             10161..11921
                     /gene="pyk"
                     /locus_tag="KPD58_002052"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBG0265818.1"
                     /translation="MLNNVKKTKIVCTLGPASQSEEVLTQLMQNGLNVCRFNFSHGSH
                     EEHKERIDMAKKVREKLNKPVAILLDTKGPEIRTGNFEDPEVFLEEGQKFTITMKDIM
                     GTKEMCTVSYKGLAEDVKSGDSILIDDGLVGLRVKEINGEDIVCVVENSGIVKNHKGV
                     NVPGVKINLPAITPKDISDIEFGISQGIDYIAASFVRKASDVLAIREVLENNNATDIQ
                     IISKIENQEGVENLDEILKVSDGIMVARGDLGVEIPTEEMPIVQKMMIKKCNELAKPV
                     VTATQMLDSMIRNPRPTRAEVTDVANAIYDGTDAIMLSGETAAGKYPVEAVKMMATIA
                     KRTEETLDYDRLLKENGTNNVTVTDAISHATCTTAVDLNASAIITSTSSGYTARMVSK
                     FRPKSPIIATTNNEKTMNKLALTWGVYPVKSSVAGNTDEVIEKAIEAARQADYIDNGE
                     LVVITAGVPVGVSGTTNLIKVHVISEEIVQGIGVGTQTVEGKVRIIKNGNACVDFNEG
                     DILVSTATDAEMNNYIEKAGAVVTENGGMTSHTAIVGINLDIPVIVSAADITNLVKDG
                     EVVTVDASRGVVYRGSTRVL"
     gene            12096..13454
                     /gene="rlmD"
                     /locus_tag="KPD58_002053"
     CDS             12096..13454
                     /gene="rlmD"
                     /locus_tag="KPD58_002053"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBG0265819.1"
                     /translation="MLSKNEMYIVDIVDIGQGGVGVGKYNGITVFVEGGLIHDKVKVR
                     INKVKKNYAVGDIVEIIEKSPFRVKRVCSDNLRDCGGCQIQDLDYNKQLEMKTNEVKQ
                     VLSRIGKLEDVEVHDTIGMDNPYRYRNKAQFPIQKNNGIPIIGFYKKKTHDVISTEKC
                     VIQHEINDKIVKIIKTYIRAYKVSIYDEKTHTGLIRHLVTKIGFTTKEVMVVLVANGN
                     KLPYLKELASVLKENIPGFKTLVVNVNQKDTNAILGRENIVIYGDGKINDYIGDLVFE
                     ISPLSFFQVNPVQTKVLYDKALEYANLNDTDTVFDIYCGIGTISLFLAQKAKKVYGIE
                     IIDDAIKDAKINAKLNNIHNAEFYVGKAEEIVPKMYREGKTANVVVVDPPRKGCDEKV
                     LETIVSMNPERIVYVSCNPSTLARDLDYLNVHGFKCVEAQPVDMFPHSTHVETVAKII
                     KQ"
BASE COUNT         5553 a         1480 c         2728 g         4073 t
ORIGIN      
        1 aatataaaat ccatatgata agcaaaatca tatattattg aatatagtta atactatata
       61 ctaatgtaat ttaaataata atgacagcta aagttcagta taatttggga ggaaatacaa
      121 tgaataatca agatatatat ataaatttat taaatatatt aagtaaagag gatataaaag
      181 tcgatgaacc tatgaaaaaa catatttcat ttagggtagg aggaccagca gatatattag
      241 ttagacctag aactgaagaa caattaaaaa atgtacttaa gttggtaaaa gaagaatcta
      301 ttccgtattt aataattgga aatggttcaa acattttaat taaagatggt ggaataagag
      361 gagtggtaat agaactagct gataatttca attcttatga gataaatgat actaggatga
      421 ctgctcagtc aggagcttta ttatcagttt tagggaaagc tctccaaaag caagaattaa
      481 aagggtttga gtttgcatca ggaatacctg gcacattggg aggagcatta gccatgaatg
      541 ctggagcata tggtggtgaa atgaaggaca tagtgaagtc agtaagactg atggatatgg
      601 aaggcaatat atttgaatta tcaaatgaac agatggaatt tgggtataga aaaagtataa
      661 tatcgaaaaa cggatatata gctttatcag cagaattaga attacaagaa ggaaattatg
      721 atgaaataaa aagtttaatg gatgatttag cgacgagaag aataactaaa caaccattaa
      781 attttgctag tgcaggaagt acatttaaga gaccaacagg atattttgca ggtaaattaa
      841 tagaagaaac tggattgaga ggtcttactt taagaggagc acaagtatct gagaagcatt
      901 gtggatttgt agtcaatcaa ggagaagcaa gtgctaaaga tattttggat ttaatatatg
      961 ttataaaaag tgctgtatat gccaagtttg gggttatgtt ggaagaagaa gtcaaaatac
     1021 ttggagagga ttaacaaatg aaaatactgg ctgattatca tacacataca ttatatagtc
     1081 atggcaaagg gactattgag gataatgtaa aggtagccat ttctaaagga ataaaaacaa
     1141 taggaatatc tgaccatagt tataaacata ttgcatatgg tgtaaagaaa aaagatattc
     1201 tcaaaatgag agaagaagta gattatttaa atgataagta cactgatata aatatacttt
     1261 taggtatgga gtgtaacata ttagatgata aaggaaatat agatatggat gataaattta
     1321 ttagagattt tgattatatt atggctggat atcattttgg ttctactcca acatcattaa
     1381 gaagcttatt aaatcattgt aataactata tttttaaaac tgaaaaagca aaagaatata
     1441 atacaaaagc tgtaataaat gcgataagta ataatgatat ttttataatt actcatccag
     1501 gagataaagg cgatgtgtat atagaagaag tagcaaagac agcaaaaaag acaaatacta
     1561 gacttgagat aaatagtagc cattcttttt tgaatgtaaa tcaattaaat aaaataaaag
     1621 ggctagaaaa taaatttata ataggctcag atgcacattg tccagaaaga gttggtgatt
     1681 ttgaactagc tatgaagact atatctgact ctaatctgga tatatcgtta gtagaaaaca
     1741 ttataatata ataaacataa gataaaaggg aggagttagc aatgaaattt gttatagtta
     1801 caggtctttc aggatctgga aaaagtgaga caatgagagc tttagaggat atgggatttt
     1861 attgtgtaga taatctacct cctgctttga taactaaatt tgcagaatta tgttatcaac
     1921 caaattcaag tattgataaa gttgctctag ggatagatat aagagggaga aagttttttg
     1981 aagcgcttca tgaaagctta aattatttag aaaaagaaaa ttatgaatat gaaatggtat
     2041 atttggattg taatgatgat gtattgttaa agagatataa aatgacaaga agaaatcatc
     2101 cattggcaaa agatatgcag attccagaag gtataaaaat ggaaagaaag ataatggaac
     2161 ctttaaagga gttatctaca tgtataattg atacaactaa catgaaacct aaagacctta
     2221 aggaagaaat aaagaaaata tattcttctg gtgaagataa tccaaaccta actatttctg
     2281 tagtatcttt tggatttaaa catggtatac ttgctgatgc agatttagta tttgatgtaa
     2341 gatttcttcc aaatccctat tatgttgaag agttaagagc taaaactggt gatgataaag
     2401 aagttagaga ttatgttatg aattctaaaa tcagtgaaga attttatgtg aaacttttag
     2461 atatgattca ttttttagta cctcaatata tagaggaagg aaaacaacat ctagtaattg
     2521 gtgttggatg cacaggtgga agacataggt cagttactat aacaaatctt atagcggagg
     2581 atttatctaa taagggatat agagttgtaa agaaacatag agactctatg ttgcgataat
     2641 ggaggcaaac atggctaaat tacttattat tgtaggaatt ttaggttgga ttgcattaat
     2701 tgtatcgtta tttatatata aaaaagcaaa aaatgaaaaa gtaaaactat ttcaaaggtc
     2761 tattgttgaa aatagagaac caaatgtggt tgtgataggt ggaggtactg gtcaatcggt
     2821 atttttaaga ggtcttaaac acactacgca aaatataact gcaattgtaa ctgttgctga
     2881 tgatggtgga ggttcaggtg tattgagaga agacttagga atgctcccac caggagacat
     2941 aagaaactgt ttattagcac ttgcaaatat agagccaaca atgaatgaag ttatgcaata
     3001 taggtttaca gaagggcttt taaaaggtca gagttttgga aatttgtttt tggctgctat
     3061 gaatgggttg tatggaaact ttgaaaaagc tgtatataaa ttaagtgaaa tttttgctat
     3121 aacaggaaga gttttaccag taactttgga agatgtaaat ttagttgcca aattagaaaa
     3181 tggcaatata ataaatggag aatctagtat acctgaagag tcaaaaattc aaaaaagctc
     3241 tattgataaa atttttttaa atccaaaaga tgtaaaacca ttaaaggatg ttattgcatc
     3301 tatttatgat gctgacatta taattatggg tcctggaagt ttatatacaa gtataatacc
     3361 caatttactt gtagagggga ttgtagacgc tattaaatcg tctgtagctc caaaagtata
     3421 tatagcaaat ataatgactc aaccaggtga gacagaaggg tataatgtat tagagcatgt
     3481 taatgctata gtaaaacaca ctgatgagaa tttaatagat tatgtaatcg caaataatga
     3541 gatattgcca gaagaaatgc ttgacttata taaacaagat ggagcagagc aagtcttgtt
     3601 agataaaaag caaaaagata aattaaaaga gatgggtata aaaactgtag agaagaattt
     3661 aattgaaata aaaaataatt atataagaca tgatgcaaag tatatatcta acatagttat
     3721 tgagttagcc ctaaatcata attacaacaa aagttagtaa aaaaggattt taaaaaattg
     3781 tgtagaaatc cttatcatta tgttagtttt aaggagtgaa catcgatgag agaagaggtt
     3841 agtgctggtg gtgtagtcct gtttggcaat acgattcttt tactaaggaa gttcaatgga
     3901 gattgggtgt tacccaaggg aaaagtagaa gaaggggaaa ataaccaaga ggcagcatta
     3961 cgtgaagtaa gtgaagaaac tggagtaaaa gcagatatat tgaaatattt aggtgaaata
     4021 cactatacat tcaaagaaaa ctgggatgaa aatagagcag ttcataaaac agttttttgg
     4081 tatttgatgc aagcaaagaa tatggatact attccacaaa aagaggaagg ctttattgat
     4141 gctaaattta tccacctaga tagagtagta gacttagcta ggtatgatga tgaaaaggag
     4201 ataataaagg ttgccttaca agaaataaaa aaaaggttaa agaaaaatta gttaataggt
     4261 gatagatatg tctttttcaa ctgaaaccaa aaatgaacta gcaagagtta tatctgggaa
     4321 tgaatgttgt aatatagctg aattgtcagc tctagttaaa tcagggggaa gtatacaaat
     4381 tgttggatat aagaagctaa atctaaagat aacaacagag ttaaattcaa tagcccgtaa
     4441 agtatttaaa ttattaaaaa agaattttaa tatcaatact acaatctcag taaataagaa
     4501 tcaaatgcta aagagaaata acagttatgt attaatggta acaagcgaaa tgggttcaga
     4561 aatgttatta aaaaagttag gtatacttga agataaagaa ggatttttta ctataaacaa
     4621 aatttcagag aacttaatta agcatgaagg atgtaagaga gctttcataa gaggagcttt
     4681 tcttggtgga ggttctataa gtgacccaga aaagaactat catatggaat ttgtaactaa
     4741 taatgaggag tttgcagatt ctctaaaaga acttataaac tctttaggtt ttaatagtaa
     4801 aattgtggct agaaaaaata atcacgttgt ttatatcaaa gagagtgaac aaatatcaga
     4861 tttgttgagt attataggag gacaccacgc tcttttaagt cttcaaaata ctaaaatagt
     4921 aaaagaaatg agaaataatg taaacaggat agtaaattgt gagacagcta acctttctaa
     4981 aacagtaaat gcagcagtaa gacaagtaga aaatattaag ctcatacaag agactatagg
     5041 aattagcagt ttgccagaaa atttacaaga gatagctaag attagaatcg agtatgagga
     5101 tatgactctc aaagaacttg gagagatgtt agaaccacca ataggaaaat ctggagtcaa
     5161 tcatagactt agaaaaatag aagaaattgc aacagatttg aaaaaaaaat catagcaata
     5221 aattgagggg acaatctcag aataagcatt aatttataac atataatggt atacgttgaa
     5281 gttaattcct attttgagat aaaagtctct gtttttgttt tagtacatat atttgtggta
     5341 aaattaaaat tataaagcag gaggtgattt aatgaacaaa gattttgctc atcttcatgt
     5401 acatactgag tatagtttac ttgatgggtt ttcgagggtg aaaaaactta taaaaagggc
     5461 taaggaacta aacatgagct caatagctat aactgaccat ggttgtatgt ttggtgttat
     5521 agatttctac aaaacagcta taaaagaagg aataaagcca attatagggt gcgaagtcta
     5581 tacagctgca cgtggattga gagataaaga cccaaactat gacaaatatc aaggacattt
     5641 agttctgtta gctaagaata tggaaggata taaaaattta ataaaaatag tttcaacttc
     5701 atacgtagaa ggtttttact ataagcctag agttgacatg gaagaactta aaaagcataa
     5761 caaagggatt atagcacttt ctgcatgcct tgcaggagat gtagcacgtg ctttaatgaa
     5821 tagaaattat gaaaaagcaa aacaatttgc tcttgactat agagacatat ttggagaaga
     5881 aaatttcttt ttggagattc aagatcacaa tttacctgag caaaaagaag taaactctgg
     5941 tttagtaaaa ttatcaaaag agactggtat accactagtt gctactaatg atgttcacta
     6001 tgtaaataaa gaagattcga aaatacatga tgtacttatg tgtattcaaa tgggaaaaac
     6061 attaaatgac ccaaatagaa tgagatttgg tagtgatgag ttttatttaa aatcaagaga
     6121 agaaatggaa gaattatttc catatgcatt agaggctatt gataatactg tgaaaatagc
     6181 tgatatgtgt aatgtggagt ttgattttaa cactatacat cttcctaagt atgatgttcc
     6241 agaaggatat acaccagaaa cttatttaag ggagttgtgt tttaatggat taaaagagag
     6301 atatgataat cctagcaatg agatactaga gagattaaat tatgaattag atgtaattga
     6361 aaaaatgggt tatgtagaat attttttaat tgtttgggat tttataaatt tttctaaaga
     6421 aaataatatt atagtaggac caggtagagg tagtgcggct ggttcgatag tagcatatac
     6481 acttaaaata actgatatag acccaataaa gtactctcta ttatttgagc gttttttaaa
     6541 tccagaacgt atatccatgc cagatataga tatagatttt tgttatgaga gaagagaaga
     6601 agttatagat tatgtaaaga gaaaatatgg tgatgaccat gtagcccaga taataacttt
     6661 tggaacaatg ggtgcaaaag ctgcaataag agatgttgga agggtattgg atataggcta
     6721 caataaagtt gacaaggtag ccaaggaaat accatttgct ttaggaatga caatagataa
     6781 ggccctagat acaaatccta atcttaaaga attgtatgac caagacccgg aaacaaaaga
     6841 aataataaat atatcaaaac agatagaggg aatgctaaga catgcttcta ctcatgcagc
     6901 aggagttgtt atatcaaaaa atcccgtaga tgaatatgta cctttgtaca aacatcaaga
     6961 tgcaatcaca acacagttta ctatgacaac acttgaagaa cttgggcttt taaagatgga
     7021 ttttcttggg ctaagaactc ttacagttat aagagatact cttgatttaa tagaaaaaaa
     7081 tagagttata aaaaactaca cagaaaaaat agacttttca aagatggatt atgacgaccc
     7141 taaagtatat gaaatgcttt catcaggaaa tacactaggg gtgtttcaat tggagagtgc
     7201 tggcatgaga agttttatga aacagctaaa accagacaat tttgaagata ttgtagcggg
     7261 aatatcccta tttagaccag gtccaatgga ttctatacct gcatatataa aaaataaaag
     7321 caacccaaaa gatgtaacat atctacatga aaaattaaag cctataatgg aagttacata
     7381 tggttgtttg gtatatcaag agcaggttat gcaggtcgtt agagatttag gtggttatag
     7441 ttacggacga agtgacctag ttagaagagc catgagtaag aaaaagatgg acgtgatgga
     7501 agaagaaaga caatatttta ttcatggtaa atttgatgaa gatggtaata tagaaattgc
     7561 agggtgtatc agaaatggtg tagaagagga aatagctaat aaaatatttg atgatatgat
     7621 agacttcgct agatatgcat ttaataaatc acatgcagct gcatatgggg ttttagcata
     7681 tgagacagct tatctaaaaa catattatcc agttgaattt atggcagcat tgataaccag
     7741 tgtaatggga aatactgata aggtagttga gtatataaga gaatgtaaag cactagaaat
     7801 agatgtatta cctccagata taaacaaaag tttttcaaag ttttcagttg aaggcgacaa
     7861 tataagattt ggtctagcag ccgtaaaaaa tgttggcgta aatataataa acaatataat
     7921 cgctgaaaga gaatctaatg gattatttgt agatttagta gatttagtca aaagattaga
     7981 ccaaaaagat acaaataaga gagtaataga aagtctaata aaatgtggag cttttgataa
     8041 tataagtgaa aatagagcta gtctgatggc tggttatgaa agtttattag aaagtgtatc
     8101 tatggataga aagaaaaatg ttcaaggtca aatttcttta tttgatgctt ttggagatag
     8161 tgaggaagat agtgattttc aacaaatata cagtttgcct aaaaggcaag agtacgaaga
     8221 aagagagaga cttaaccttg aaaaagaagt cttgggaatg tatgtaagtg gacatccttt
     8281 atcacaattt gaaaaagaac tacaagaaaa aacatctatg gataatggaa aattaaattc
     8341 attaaaagag gaagaagagt catacctaca aatgaatgaa acagatgtta taatgggtgg
     8401 aatgattgta actaaaacta ttaaaacaac aaaacgaaat gaaattatgg catttattga
     8461 attagaagat ttgtatggtg ctatagaaat aatagttttt cctcaattat tacaaaagta
     8521 taatgtcatt ttgaatgaag ataatataat atatgtaaaa ggaacattaa gtataaaaga
     8581 aggcgaaaat gcaaaactta tagctagaga gataaaagat ataaatgatg atactgactt
     8641 tgcagataga aactacaatt caggaagaac tagaaatgta gaaaatatta agccagaaag
     8701 tggtaaaaaa ttgtatttga aaatagatag tatgtctgat actgatacaa ctaataatat
     8761 tgtagaaata gccaaacaat atccaggtaa tgacagtata tacttgtacc ctatggatga
     8821 aaatataaat ggtaaacgta aagcatataa aatgaccggg gcattgactt gtatatgtga
     8881 tgaatttata aataacttag aagagttact tcctaaagat aacataaaag taaaagcttg
     8941 attttttata taaaaatcta aatatattat aataatatga aaaatattgt ccgttttgtg
     9001 atataatatc acaaaatgga catttaagga catttaattt ttttagtctt gatgggatta
     9061 aaaagtgata tagggacaaa aaaagtccat tagaatacaa taacaatgat tataataaaa
     9121 aattgacata aaatccaagg aggtaaaaaa tgaaaactat aggattacta acaagtggag
     9181 gagatgctcc aggaatgaat gctgcgatta gagcggttgt aagatcagca atatattatg
     9241 gatgcaaagt atatggaatc aatagaggat ataagggatt actagaagaa gatttaactg
     9301 aaatgaactt atcttctgtt ggagatatta tacacagggg aggaactata ttaaaatctt
     9361 ccagatgtga agaatttaaa acagaagaag gtagattaaa agcagtaaaa attcttaaaa
     9421 agtataaaat tgattgctta gttgtaattg gtggagatgg ttcatttgca ggagcccaaa
     9481 aacttagtga cttaggattc ccagctatag gcataccagg aactatagat aacgatttag
     9541 catatacaga ttatactata ggatttgata ctgcaatgaa tactattata gatgctatag
     9601 gtaagataag agatacatca tcatctcatg aaagagttaa catagtagaa gttatgggaa
     9661 gacactgtgg agatttagcc ttatatgcag gtcttgcagg aggagcggaa actataatag
     9721 taccagaagt agaaattaca gtagatgaag ttgctctaag attaaagact actcaaaaaa
     9781 gaggtaagag acatagtata atcgttttag cagaaggagt cggaagtgct agtgatttag
     9841 aaaaggaact taaaaaagag tcaggagcag atttaagagt cactgtattg gggcatgtcc
     9901 aaagaggagg aagcccaact gtatctgata gaatactagc aagtagactt ggagtaagag
     9961 cagtagaatt attattagat ggaaaatcag ctagagttgt aggaataaaa gaaaacaaaa
    10021 taattgattt agaaataagt gaagctttag ctcaaaagaa agtatttgat aaagaagctt
    10081 atgagatggc aaaaatatta tctatataag ttttagtatt aaaaataaat ctgtggaata
    10141 atatctcagg aggagcaaat atgttaaata atgtaaagaa gacaaaaatt gtttgtacac
    10201 taggtccagc atcacaaagt gaggaagttt taactcaact tatgcaaaat ggtctaaatg
    10261 tgtgtagatt taacttttct cacggttcgc atgaagaaca taaagaaaga attgatatgg
    10321 caaagaaagt tagagaaaaa ttaaataagc cagttgctat attattagat actaaaggtc
    10381 cagaaattag aacaggtaac tttgaagacc cagaggtatt tttagaagaa gggcaaaagt
    10441 tcactataac tatgaaagat attatgggga ctaaagaaat gtgtacagta agctataaag
    10501 gtcttgctga agacgttaaa tcaggagatt ctatactaat agatgatggt ttagttggct
    10561 taagagtaaa agaaataaat ggagaagata tagtttgtgt tgttgaaaat tcaggaatag
    10621 ttaaaaatca taaaggagta aatgtacctg gtgttaagat aaatttacca gcaataactc
    10681 ctaaagatat aagtgatata gagtttggaa taagtcaagg aatagattat atagctgctt
    10741 cttttgtaag aaaggcatct gatgtactag caataagaga agtattagag aacaataatg
    10801 caactgatat acaaataata tctaaaatag aaaaccaaga aggtgtagag aacctagatg
    10861 aaatattaaa agtttctgat ggtataatgg tggctagagg tgacttaggt gttgagatac
    10921 caactgaaga gatgccaata gttcaaaaaa tgatgataaa aaaatgtaat gaattagcaa
    10981 agccagttgt tactgccact caaatgcttg attctatgat aagaaatcca agaccaacaa
    11041 gagcagaagt tacagacgtt gccaatgcta tatatgatgg aacagatgcg ataatgttat
    11101 ctggagaaac tgctgctgga aaatatcctg tagaggcagt aaaaatgatg gctacaatag
    11161 caaaaagaac agaagagact ttagattatg atagattact aaaggaaaat ggtactaaca
    11221 atgttactgt aacagatgca ataagccatg caacttgtac aacagcagta gatttaaatg
    11281 catcagctat aataacatct acttcttcag gatatactgc tagaatggta tctaaattta
    11341 gaccaaaatc tccaataata gctactacaa ataatgaaaa gactatgaat aaattagctc
    11401 ttacatgggg agtatatcca gttaagtctt ctgttgcagg aaatacagat gaagttatag
    11461 aaaaggcaat tgaggcagct agacaagctg attatataga taatggggaa ttagtagtta
    11521 taactgctgg agtaccagta ggagtaagtg gaactactaa tttaataaag gttcatgtaa
    11581 taagcgaaga aatcgtgcaa ggtataggtg taggtactca aacggtagaa ggtaaagttc
    11641 gtataataaa gaatggaaat gcttgtgtag actttaatga aggcgacata cttgtttcta
    11701 ctgctactga tgctgaaatg aacaattata tagaaaaagc aggagctgta gtaactgaga
    11761 atggtggaat gacatcacat acagctattg taggtataaa tttagatata ccagttattg
    11821 tgtctgcagc tgatataaca aatctagtaa aagatggaga agttgtaact gtggatgcat
    11881 caagaggcgt ggtatataga ggttcaacta gagttttata agaaaaataa taaatttatt
    11941 tactaagggg ctttataagc tccttttttt ttggtaaaat aaaatattgt agcttagtaa
    12001 aaaatatagt ttaattaata gcaatatttt aatataaatt taaagaaaaa agtttgtaaa
    12061 gatttaaata taatagattg attggagggt gatttatgct ttcgaaaaac gagatgtata
    12121 ttgtagatat agtagacatt ggtcaaggcg gagtaggagt aggaaagtac aatggtatta
    12181 cagtttttgt agagggcgga ttaatacatg ataaagttaa agttaggatt aataaagtaa
    12241 agaaaaatta tgctgtggga gatattgttg agataataga aaagtctcca ttcagggtga
    12301 aaagagtgtg tagtgacaat cttagggatt gtggagggtg tcagatacaa gacttagatt
    12361 ataataaaca acttgaaatg aaaacaaatg aagttaaaca agtactttcg agaataggta
    12421 agttagagga tgtagaagtt catgacacta taggaatgga taatccatat agatatagaa
    12481 ataaagcaca atttccaata caaaagaata atggtattcc aattattggc ttctataaaa
    12541 agaagactca tgatgtaata tcgacagaaa aatgcgtaat acaacatgag ataaatgata
    12601 aaattgttaa aataataaaa acatatataa gggcctataa ggtaagtata tatgatgaaa
    12661 agactcacac ggggttgatt aggcatttag taacaaaaat aggttttaca actaaagaag
    12721 taatggttgt attagtagca aatggaaaca aacttccata tcttaaagaa ttagcttcag
    12781 tgcttaagga aaatatacct ggatttaaga ctttggttgt aaatgtaaat caaaaagata
    12841 cgaatgctat tctaggaaga gaaaatatag ttatatatgg agatggaaaa ataaacgatt
    12901 atataggtga cttagtgttt gagatttcac cattatcatt tttccaagta aaccctgtcc
    12961 aaactaaagt attgtatgat aaggctcttg aatatgcaaa tttaaatgat actgatactg
    13021 tttttgatat ttactgtggg ataggtacaa tatctttatt tttggcacaa aaggctaaaa
    13081 aggtttatgg aatagaaatt atagatgatg ccataaaaga tgctaaaata aatgctaaat
    13141 taaacaatat acataatgct gaattttatg ttggaaaggc agaggaaatt gtacctaaga
    13201 tgtatagaga gggtaagact gccaatgtag tagttgtaga cccacctaga aagggatgtg
    13261 acgaaaaggt attagagact attgtttcaa tgaatccgga aagaattgtc tatgtttctt
    13321 gtaatccttc tactttagca agggatttag attatctaaa tgttcacgga tttaaatgtg
    13381 ttgaggcaca acctgtagat atgttccctc attctacaca tgtggaaact gtagctaaga
    13441 taatcaaaca atagtaatgt ttatatagat tatatactaa taatatattg tatttgccac
    13501 cgatttgcca ctgtaaagcg attttggtgg caaatttagt ctacaatgtt atcaaaaatc
    13561 tctaaagttt tatttttaat tctataaaac ttattataat tgtaatttta aaagaaatcg
    13621 tcaatctaca gtagccagat tttatacaac ttattttttt catagagtaa tttacataat
    13681 ctatagttga atattattta atatttttat attataattc attagcaaat ataggcatta
    13741 caaatgttga taaacatgtg aaagcacata tccacttata attatttttt ttaaaaccat
    13801 aaaatcacct cttcatttgt tgtatgtttt tata
//
LOCUS       DAELLV010000091        13653 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.091, whole genome shotgun sequence.
ACCESSION   DAELLV010000091 DAELLV010000000
VERSION     DAELLV010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13653
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.091"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(669..1298)
                     /locus_tag="KPD58_002054"
     CDS             complement(669..1298)
                     /locus_tag="KPD58_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HBG0265820.1"
                     /translation="MKNRKVYMYVFDTMSDWEVAYLTAELNTGRYFKKGLKPLKVITV
                     GIDKNPIITMGGLKVLPEITVDEFNMESKDLLILPGGNTWLSMNHEIILEKVKEALRQ
                     DSIVAAICGATLGLAKKGLLDFRNHTSNDLEFLKMVIPNYSGEKYYKTESVVSDENLV
                     TASGIAPLEFTFQVLKLLDVFESEALNSWLNLYKTHDSKYFFELMNCNK"
     gene            complement(1377..2339)
                     /locus_tag="KPD58_002055"
     CDS             complement(1377..2339)
                     /locus_tag="KPD58_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017561613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBG0265821.1"
                     /translation="MAKIDNMMSILWMLNSDKKITAKQIAEKLEINIRTVYRYIDVLS
                     ASGVPIISDTGHNGGYSLLNNFTKAPLFFDIEEQKSLLHAALFAKKEGYFLDEALDKS
                     ILKLKMYSNQKQEDILTNHLRALEVIKPIGKVSIESVLKKLENSILKELSVEINYHAG
                     RKVKSKYRIINPYGIVHWNNNWYVVAFCNMRNEVRSFRVDRISEIIETSNTFNRPTEF
                     SASEFFIKGIIPEIKDEQNIIQLVIEGSIDSLDSLSQHWFLGHYLKERTPNKIIFMLE
                     EEVICKYIPNILLPYRKTIQVIEPLSLRKKIIEDLLELIDYYQI"
     gene            complement(3101..6697)
                     /gene="cbpA"
                     /locus_tag="KPD58_002056"
     CDS             complement(3101..6697)
                     /gene="cbpA"
                     /locus_tag="KPD58_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding adhesin CbpA"
                     /protein_id="HBG0265822.1"
                     /translation="MQQKCKKKALSMILSISMGLGSVASFNASNTFADTIEESTNAGN
                     AEGDTNAGNAGGDANAGNAEGDANAGNAGGDTNVGNPEGDANAGNAGGDANAGNAEED
                     ANAGNAEEDANAGNAEGDANAGNAEEDSNAGNAEEDSNAGNAEGDMNALVVEEATAED
                     PTSVGDFKISGGEINVDFKFASGVLTILSGKQMTIQNNNPNTPTTHRIVIPKDVKAEL
                     IFDGVNIKTASNSPFTLTPDKDGGAYAHVILQDGSENKLESGDVYYPGMRAGKGTTLI
                     IDDSVVNRDIEGEFIVSEGGKIPRDVTLENGDKLKKGDRLTLLDSNNRGSLKVVGNYY
                     SAGIGGGNTESGGNIIINGGAITAIGQESSRNTGYGAGIGGGGNGSGGDITINGGTIT
                     AKGAYHGAGIGGGYYTTTKNALSPSVVDPSTGSGYSGNITINGGLSYSYGGAHGSAFG
                     DGCVNPGTNRGYRILVTGGTILPYMTGVGDWIFDLSATTGDVIVTGGSLKATTFKSLG
                     GAVAYGDLEKKTKVFMNKISLTYLGQDKVSTTLVDDFKMLIDGKIYPYGAPSYTDAQG
                     ILYLWFSDKFTGSEVSVNLVVIDKITGEVLKPDDFYIKDIGSGSQFLKQYEEFTIDDT
                     PPYLIIKRYDGLPFEEDVEKSFLDWLLKDGGIPITTPKGEKLTDKKFMTISSQRLNEE
                     TMEPDINLGSDDLTKQVDAGKYQLSITSTQFSSIYPFKDAYWGHRAYYKAEIIPADTE
                     TSLVVEESKAKTDVFKPTDTFTLNVTISPDKKEGPDCASPRGYVQFYINGKEYKDPVE
                     LKTHNKGSESHNYSTASIEWRPLDSNHHIFTTEQTITVKYIGDDINYIEGSEDEKKLK
                     LDMTNVDVDGDGIPDINIDTDGDGRPDINIDTSGDWKPDINIDTDNTGEWKPSTEGGN
                     GDGIWKPDKNIDTNGDGNPDTDYNRPAIDTDNDGVDDYWKPDKNVDTGSGGYDTGNPN
                     LNKPDPDNGGNSKPDSGNGGNSKPDSGNGGNSKPDSGNGGNSKPDSGNGGNSKPDPDN
                     GGNSKPDPDNGGNGKPDPDNGGNSKPDPDNGGNSKPDPDNGGNSKPDIDTDGDGKPNI
                     NIDTDGDGKPDINIDTDGDGKPDINIDTDGDGKPDINIDIDGDGKPDINIDTDGNGKP
                     DINIDTDESLDSNVGQNVQTGDQSNIMLDLALMFISLFFLIKNLTNKYLRRK"
     gene            6966..7538
                     /locus_tag="KPD58_002057"
     CDS             6966..7538
                     /locus_tag="KPD58_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HBG0265823.1"
                     /translation="MKKLVKILINIICIITLIFSSLSIYIKLSEYKKADEVYTELREN
                     TINNSKYQELYDKNNDYRFWLKINNVNIDYPVVQGYNNDFYLTHDFYKNYLPLGSIFM
                     DYRNNFENDKSLIVYGHYMKNKTMFGQLENYTDEVFFKENNLVEINYKVQTYTYEIFS
                     VYTADLINRDYLSIHFNNNDEFKYSLNYIT"
     gene            complement(8551..9213)
                     /locus_tag="KPD58_002058"
     CDS             complement(8551..9213)
                     /locus_tag="KPD58_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0265824.1"
                     /translation="MKMLKVIDIKKIYGKGENEIKAVDGITLEIEPHKFSAIIGQSGS
                     GKSTLLHCMAGLDKPTLGNVFMDNLDLYTLSDEKLSNLRSKEFGFIFQNYNLIPVINV
                     YDNIVLPILIAKGKIDNNYINDLITKLGLESQINKFPNELSGGQQQRVAIARSLANKP
                     SIIFADEPTGNLDSKTTNEVMNLLKYCVNEYKQTLVMITHNDEIAKMADCIITISDGK
                     VI"
     gene            complement(9230..11785)
                     /locus_tag="KPD58_002059"
     CDS             complement(9230..11785)
                     /locus_tag="KPD58_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG0265825.1"
                     /translation="MGIYLKMSLAYLKKNKLRTLLLIIGVMLGVMLIFGPSVIKDSQN
                     ENNVVAIHELYSGYHVEFSDLTLDDAKKLETDEKVSKGNSVQNLGRISDKKGNSFILT
                     SSNEEYLKSRYNKIIEGRLPKNNNEIVLEKKALEAMNLSEGVGSTLNLTIKKNYKDTN
                     GSNRLFSINKEFKIVGVMEKPKGYYDVVWQYEAVTYGNDETNNIIPHDAISYNSILSF
                     KSGWRNIEGECDYLMKKYKLGQKSFLPNVPLVRELRYVEDDKNDPNVFKRNVLITTVS
                     IIFIFNIFNITLNQSVKEMGLFRLMGAKKRNVRCMIIYQALIITIIGILLGLVAGVTY
                     SYIGISNYNEVLYSEAGLNPKLYINNEIVIKAILVGIISVFISCIIPTFKIGKISPIE
                     SIINTDKIKNHRSRLGIDRLVSKIFGFYGFMGLKNIGRNKVRAAISMLSIALGGYIFI
                     TTFSSMQEEVNSKIEDMQNRYNITMQFGVNADPQISKYTDSDVENIEKIKGVKSIHKI
                     QITNGMFTFNNKDINKEFTTYNGVEKKEIMEDKLVLKLYDDDYIDEKLNDFVEEGSLD
                     DIKKDTDGYPNVAVYNYFYDIVDDHTIKPIFKELNIGDIITVKIPVEENNTIVYKEMK
                     VRVAVILKDEWIAMGDGDFMPDLEIITSNIHSKNITGEQKYTQLGINLENPYDEIVNN
                     EIQKISRSIPGSLFNSRLSYHEMSESIYKSYIKSQIAIIALVLIIASINIFCTIRTNL
                     LIRKKEISTLRALGVSLKNMKKMLIYEALAYAVFSFIIAMIPSFINLVKFTNWNNDAY
                     INYGIENFMSFTFPIKESLLFFAISVFVCLIAVVVSNRDFESMNIIEGIKENE"
     gene            complement(12018..12212)
                     /locus_tag="KPD58_002060"
     CDS             complement(12018..12212)
                     /locus_tag="KPD58_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HBG0265826.1"
                     /translation="MRKYKAILLTLIISIIAIIGFIIYNNYFRKWNEAEKYIDKYMTY
                     QGVSGDDIERITKEKYTKEN"
     gene            complement(12224..12403)
                     /locus_tag="KPD58_002061"
     CDS             complement(12224..12403)
                     /locus_tag="KPD58_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265827.1"
                     /translation="MIHVEISEKEFFRKIEEVFDKGEGNIEVDNLGDIKIQVKKFDDK
                     TKSLELKLIKEFKLK"
     gene            complement(12408..12665)
                     /locus_tag="KPD58_002062"
     CDS             complement(12408..12665)
                     /locus_tag="KPD58_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265828.1"
                     /translation="MSKKISESIENSGLRVAENKSDGSFVLSLGNLEYTKEQPSQVLH
                     YSMTKDKKGTKEILSIQCVKDFSYNEKLSEDDKFIMAIYNI"
     gene            complement(12638..12778)
                     /locus_tag="KPD58_002063"
     CDS             complement(12638..12778)
                     /locus_tag="KPD58_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265829.1"
                     /translation="MVIAISLTLGLITGCTNKVSDTNEKIYTIEPISSIKKTCQRKYQ
                     NQ"
     gene            complement(12925..13653)
                     /locus_tag="KPD58_002064"
                     /pseudo
     CDS             complement(12925..13653)
                     /locus_tag="KPD58_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
BASE COUNT         4389 a         2342 c         1434 g         5488 t
ORIGIN      
        1 gtttatttca tctatcatct caatcatatc atttactaac tattattcta atgagcatat
       61 aacattgaaa cttataatta tacaacctaa atgcctccca atgtaccctc aaaacatcat
      121 ttcctgtaac tgaagtataa ttggtatatt ttatcactga aataaaaaaa cactagtcaa
      181 taaagactag tattttcaag tattttatca ttcttttttt aattattgga gacgaatcat
      241 cagaataatg cccataattt atctatatgc aaattctaaa tttaaaataa agtttcagat
      301 aaatttacta ttctatgcta tgatacaaca gaaaaaactg aaatctatga gtggtagctt
      361 catataatcc tagttcctac agtaatcaaa actgaggttt attctatatg taactaatga
      421 taaattttat tataggagtt attagatcta tttgtgttac tataagtagt aattatatat
      481 atgataattt tttaacactt ttctgttggc tatatagaaa gtggtttaga atttaaattt
      541 aaataaaatt taaattcacc aaaaagaaat ctatattaga aaccactact ctattccacc
      601 agattgtaat tttttgttct ttatatcaca atgctatacc atattaaatt gaaaaataag
      661 ttttaaattt atttgttaca attcattaat tcaaaaaaat attttgagtc atgagttttg
      721 taaagattta gccatgaatt taatgcttct gattcaaaaa catctaaaag tttcaatact
      781 tgaaacgtaa attccaacgg agcaattcct gaagccgtaa ctaaattttc atcactaact
      841 acagattccg ttttataata tttttctcca gagtaatttg gtattaccat tttaagaaac
      901 tctaagtcat tacttgtatg atttctaaaa tctaatagcc cttttttagc taggcctaat
      961 gtagctccac atatggctgc aactatagaa tcttgtctta aagcttcttt aactttttct
     1021 aatattatct catgattcat agataaccaa gtatttcccc ctggcaaaat taataaatcc
     1081 ttactttcca tattaaactc atcaactgta atttcaggta aaacttttaa cccacccatt
     1141 gttataatag gatttttatc aattcctaca gtaattactt ttagtggctt taatcctttt
     1201 ttaaaatatc ttcctgtatt taattcagca gttaaatatg ccacttccca atctgacata
     1261 gtatcaaata catacatata tacttttcta tttttcatct tttttactcc ttatgtaatt
     1321 aatttatata tctaattata aaataactta actgacatat tcagtcagtt aagctattaa
     1381 atttgataat aatcaattaa ttctagtaaa tcttcaatta ttttcttcct aaggcttagt
     1441 ggttcaatta cttgtattgt ctttctataa ggtaaaagta tatttggtat gtacttgcat
     1501 attacttcct cttcaagcat aaatattatt ttattaggtg ttctttcttt caagtagtgc
     1561 cctaaaaacc aatgttggct taaactatct aaagaatcta tactcccttc tataactaat
     1621 tgaattatat tttgttcatc ttttatttca ggtataattc cttttataaa aaattcactt
     1681 gctgaaaatt ctgtcggtct gttaaatgta tttgatgttt ctatgatttc actaatccta
     1741 tccactctga aactacgaac ctcatttctc atattacaaa atgcaactac gtaccaatta
     1801 ttattccagt gaacaattcc atatggattt ataattctat attttgattt tactttacga
     1861 cctgcatgat agttgatttc tacagatagc tcctttaata tactattctc caatttttta
     1921 agtactgatt ctatagacac tttcccaatt ggttttatta cttcaagggc tcttaagtgg
     1981 tttgttaaaa tatcttcttg tttttgattt gaatacattt ttaactttaa tatcgattta
     2041 tctaatgctt cgtctaagaa gtatccttct tttttagcaa atagtgctgc atgaagtaga
     2101 gatttttgct cttctatatc aaaaaaaaga ggtgctttag taaaattatt taaaaggcta
     2161 tacccaccat tatgacctgt atctgaaatt ataggtactc cactcgcaga aagtacatca
     2221 atatatcggt acactgttct tatatttatt tctaattttt cagctatctg ttttgctgta
     2281 atttttttgt cagagtttaa catccacagt atagacatca tattgtcaat tttagccatg
     2341 tagtttcacc tctttctatt tgttatataa agttattata taactttttg attgattttt
     2401 tacaaaaaaa cattagtaaa ctgctaataa tttatactaa gatttatttc aaatatttat
     2461 agatagtctt tttctatagt taagttatcc attaaattac atagtagtat atctatttta
     2521 gttgatatat tttcagctac ctcccttgaa tattattaca ataagtctaa atcttctttc
     2581 ttccataaat atattatgag gaaagactat catctttaat ggtttaatag tataaaagat
     2641 attaatttaa aaaaatctat ttttatatca gatgggtatt gttttttata aaactgaaaa
     2701 aatcacctaa tagaatctat gattttttaa cataacctcc ataaattagt tccaattatc
     2761 acttcaaatt caaattttta tctgaagaat atacaatcat atttccatac tcaacataat
     2821 attgagtgtt tggcttgtat ttacaaatta ttattctaac aaaaacaact atacatagat
     2881 taaaatcatc ttctaaacat aactactaaa attgaattaa tgatatcaat tccattaatc
     2941 tcttttaata aaaaattctc ttttctatat gaaaattatt tcaaaacttt taagttaaat
     3001 acaaaaatga ctataaatag atttttattt acacaaatct atttatagtc actatagttt
     3061 atccaataaa ataataaaat aaatatgtac acaataaatg ttatttacgt cttaagtatt
     3121 tatttgttag atttttaatt aaaaaaaaca agctaatgaa cattaatgct aaatctagca
     3181 ttatattgct ttgatctcct gtttgtacat tttgacctac attactatct aaactttcat
     3241 ctgtatctat atttatatct ggttttccat tcccatctgt atctatattt atatccggtt
     3301 ttccatctcc atctatatct atatttatat ctggttttcc atctccatct gtatctatat
     3361 ttatatctgg ttttccatct ccatctgtgt ctatatttat atctggtttt ccatctccat
     3421 ctgtgtctat atttatattc ggttttccat ccccatctgt atctatatct ggtttgctat
     3481 ttcctccatt atctgggtct ggtttactat ttccaccgtt atctggatcc ggtttgctat
     3541 ttcctccatt atctggatct ggtttaccat tgccaccgtt atctggatct ggtttgctat
     3601 ttcctccgtt atctggatcc ggtttgctat ttcctccatt acctgaatcc ggtttgctat
     3661 ttcctccatt acctgaatcc ggtttgctat ttcctccatt acctgaatct ggtttgctat
     3721 ttcctccatt acctgaatct ggtttgctat ttcctccatt atctggatct ggcttattta
     3781 gatttggatt acctgtatca taacctcctg agccagtatc tacattttta tcaggcttcc
     3841 aataatcatc tactccatca ttatctgtat ctattgctgg acgattataa tctgtatcag
     3901 ggtttccatc tccattggta tctatatttt tatcaggttt ccaaatacca tctccatttc
     3961 caccttctgt actaggcttc cattcacctg tattatcagt atctatgtta atatctggct
     4021 tccaatcacc actcgtatct atattgatat ctggccttcc atcaccatca gtgtctatat
     4081 ttatatccgg tattccatcc ccatctacat ctacatttgt catatctaac tttaacttct
     4141 tttcatcctc actaccttca atgtaattta tatcatctcc aatatatttt acagtaatag
     4201 tttgttcagt agtaaaaata tgatgattgc tatctaaagg tctccattca atagatgcag
     4261 tactataatt atgactctca ctacccttat tatgagtttt taattctact ggatctttat
     4321 attcctttcc attaatatag aattgtacgt aacctcttgg agaggcacaa tctggtcctt
     4381 ctttcttatc tggtgaaata gtaacattta atgtaaacgt atctgttggt ttaaaaacat
     4441 cagttttagc tttagactct tctacaacta atgaagtttc tgtatcagca ggaataattt
     4501 ctgccttata atatgctcta tgcccccaat aagcatcctt aaaaggatat atactcgaaa
     4561 actgtgttga tgtaatgctt aattgatatt tcccagcatc aacttgtttt gtaagatcat
     4621 cacttcctaa atttatatct ggttccatcg tttcctcatt taaacgttga gaactaatag
     4681 tcataaattt tttatctgtg agtttttcac ctttaggagt agttattgga ataccaccat
     4741 cttttaatag ccaatctaaa aatgattttt ctacgtcttc ttcaaatggt aatccatcat
     4801 atcttttaat aatcaaatat ggtggagtat catcaatagt aaattcttca tattgcttta
     4861 aaaattgaga acctgaacct atatctttaa tataaaaatc atctggtttt agaacttctc
     4921 cagtaatttt atctataaca actaaattaa cagatacttc agatccagta aatttatcag
     4981 agaaccataa gtaaagaatc ccttgagcat ctgtataact aggtgctcca taaggataaa
     5041 tttttccatc aattagcatt ttaaaatcat caactagtgt agtactaact ttatcttgcc
     5101 ctaaatacgt tagagatatt ttattcataa aaacttttgt tttcttttct aaatcaccgt
     5161 atgcaactgc tccacctaaa cttttgaatg ttgttgcttt taatgaacca cctgttacta
     5221 taacatctcc cgttgtagca cttaggtcaa aaatccaatc tcccactcct gtcatataag
     5281 gtaaaatcgt tcctccagtt acaagtatac gatatcctct atttgttcct ggattaacac
     5341 atccatcccc aaatgcacta ccatgtgcac ctccatatga ataggaaagt ccaccattaa
     5401 ttgtaatatt cccactataa ccagaacctg tgctaggatc tactactgag ggacttaatg
     5461 cattctttgt tgttgtatag tatcctccac ctattccagc tccatggtat gcacctttag
     5521 cagttatagt accaccattg attgtgatat ctccaccact accattaccg cctccaccta
     5581 ttccagctcc atatcctgta ttacgactcg attcttgacc tatagcagtt atagcaccac
     5641 cattaataat aatatttcct ccactttcag tatttccacc gccaatacct gcagaataat
     5701 aatttccaac aacttttaaa cttcctctat tattactatc tagtagtgta aggcgatctc
     5761 ctttttttaa cttgtctcca ttttctagag ttacatctct aggaattttt ccaccctcag
     5821 aaactataaa ctcgccttca atatctctat ttacaacaga atcatcaata attagagtag
     5881 taccttttcc tgctctcata cctggataat atacatcacc tgactctagc ttgttttcac
     5941 taccatcttg taatataaca tgtgcatatg caccaccatc tttatctggt gttaaagtaa
     6001 atgggctatt agatgcagtt ttaatattta ctccatcaaa tattaattca gcttttacat
     6061 ctttaggtat aacaatccta tgtgttgttg gagtatttgg attattattt tgaattgtca
     6121 tttgttttcc actaagaata gttaaaacgc cacttgcaaa tttaaaatcg acatttattt
     6181 ctccaccact aattttaaag tctcctacag atgtaggatc ttctgcagtg gcttcttcca
     6241 caactaatgc attcatatct ccttctgcat ttcctgcatt agaatcttct tctgcatttc
     6301 ctgcattaga atcttcttct gcatttcctg cattagcatc tccttctgca tttcctgcat
     6361 tagcatcttc ttctgcattt cctgcattag catcttcttc tgcatttcct gcattagcat
     6421 ctcctcctgc atttcctgca ttagcatctc cttctggatt tcctacatta gtatctcctc
     6481 ctgcatttcc tgcattagca tccccttctg catttcctgc attagcatcc cctcctgcat
     6541 ttcctgcatt agtatctcct tctgcatttc ctgcattagt actttcttct atagtatctg
     6601 cgaaagtatt tgacgcatta aatgatgcta ctgatccaag tcccattgag atacttaaaa
     6661 tcattgatag tgctttcttt ttacattttt gttgcataaa ttccccctcc ccttaataat
     6721 ttttaatata cactgtatac atccttatat acactctaat aattcttaat atacactgta
     6781 tacatcctta tatacactct aataattttt aatatatact taaaacttat atttgtcaat
     6841 gaatatacag tttttttcaa ccttatatat tataagtatt cattatttgc taaataacta
     6901 caataaaaat tttaattata taaaatttat ttaatctttt atataataat acaaggggta
     6961 attaattgaa aaaactagta aaaatattaa taaatattat ttgcattatt acgctaattt
     7021 tctctagcct ttcgatttac ataaaattat ctgaatataa aaaagctgat gaagtatata
     7081 cggaactcag agaaaacact atcaataata gcaaatatca agaactttat gataaaaata
     7141 atgattatag attctggctt aaaataaaca acgtaaatat agactatcct gtagtacaag
     7201 gatataacaa tgatttctac ctaactcatg atttttataa aaattatctt cctttaggta
     7261 gtattttcat ggattataga aataattttg aaaatgataa gagtttaata gtctatggtc
     7321 attatatgaa aaataaaact atgtttggtc agctagaaaa ctatactgat gaagtatttt
     7381 tcaaagaaaa taatttagta gaaataaatt ataaagtaca aacttatact tatgaaattt
     7441 tttcagtata tactgctgac ttaattaaca gagattattt aagtatacac tttaataata
     7501 atgatgagtt taaatattct ttgaattata taacctaaaa atcagtacac aaatctgata
     7561 taaaggtaga tatttcagat aaaattataa ctttgtatac ttgtaaatat gaatttaaag
     7621 atgctcttac tatagttcat gctaaattaa tttcaaaaaa gagacctata agtaggtttg
     7681 tgtaaatgca gacttatcta cagtcttttt tgaacgtaaa attggtaagt atagaataat
     7741 attcatatta aacttttgta ggcaaagaaa tgaaaaatca aagagaaata attattacag
     7801 atttagatta ccaagctgaa ttaagaaagt gtaaaactat ggaggatgta gttagtgaag
     7861 ataatatata atagtttaac ttataaatta tcttggtgat attaatgaag tttcaaagta
     7921 tatattattt attgatataa ataaaattaa atctgttgct aatagatgta cctttgtttg
     7981 gagacaaact attgaagaaa tataactaaa atgttgaagt agctacctga ctttattttg
     8041 aaatgcgaac aagattttgg cattaagata atttagaaag aatcactggt acacaaggat
     8101 gtgaacttag aatgaataga agtattcagg cataaggagc tttcacccaa ataaaacata
     8161 gtatgaactt taaaagaatt ctaatcagag gcaatcaaga tattctttca gagtttatac
     8221 ctgtagtgtt agcctatagt atttctaagt ttcacaaata agataatcat taataaatgt
     8281 agtatgtata ttcatcctct caaaaatatt ttttaatctt acactttgta aattattcta
     8341 ttttaaaatg gtatttttat acctttattt cttatgaccg ttttcaagct caaattttgc
     8401 ataaatataa attaatgaaa agcaagtaaa aaaagaactg tcgcacagca atttaaaatt
     8461 actcattcga cagtccttat ttcgtggatt taaatagtgg agaagaatca caagaataat
     8521 acccacgatt aaacctttat acaatacatt ttatatgact ttaccatcac taatagttat
     8581 tatacaatca gccatcttag ctatttcatc attatgagtt atcataacca acgtctgctt
     8641 gtattcattt acacaatact ttagtaaatt catcacctca ttagttgttt tactgtctaa
     8701 atttccagta ggttcatctg caaaaattat tgatggctta ttggcaagtg atcttgctat
     8761 tgcaactctt tgttgttgac cacctgataa ttcattagga aatttattaa tctgactttc
     8821 taatcctaac ttagttatta aatcattaat ataattatta tctatctttc ctttagcaat
     8881 taaaatagga agaactatat tatcataaac attgataaca ggtattagat tatagttttg
     8941 gaatataaac ccaaattctt tacttctaag attagatagc ttttcatcac ttaaagtata
     9001 taaatctaaa ttatccataa atacattacc taaagttggt ttatcaagcc ctgccataca
     9061 gtgaagtaat gtgctttttc cagaaccact ttgtccaatt attgcactaa atttatgggg
     9121 ttctatctct aaagtaattc catcaacagc ctttatttca ttttctcctt ttccatatat
     9181 ttttttaata tctataactt ttaacatctt catacattta cctcccaaat tattcattct
     9241 cttttatacc ttcaataata ttcatacttt caaaatctct attacttaca actactgcta
     9301 ttaaacatac aaatacagat atagcaaaga atagtaaaga ttccttaatt gggaatgtaa
     9361 aagacataaa attctctatt ccatagttta tatacgcatc attattccaa tttgtaaatt
     9421 taactagatt tataaatgat ggaatcatag ctattataaa gctaaaaact gcatatgcta
     9481 atgcttcata tataagcatt tttttcatat ttttaagact aacacctaat gctctaagcg
     9541 ttgaaatttc tttttttcta attagtagat ttgttcttat agtacagaaa atattaattg
     9601 atgctattat taaaactagt gcaattattg ctatttgtga tttaatgtaa cttttatata
     9661 tactttctga catttcatga taacttaatc tactattaaa tagagaccct ggtatacttc
     9721 ttgaaatttt ctgtatctca ttattaacaa tctcatcata tggattttct aaatttatgc
     9781 ctaattgagt gtatttttgt tctcctgtta tgtttttaga gtgaatatta cttgttataa
     9841 tttctagatc tggcataaaa tcgccatctc ccatagcaat ccattcatcc tttaaaatta
     9901 cagccactct taccttcatc tctttataaa caattgtatt attttcttca acaggaattt
     9961 ttacagttat tatgtctcct atatttaatt ctttgaatat aggctttata gtatgatcat
    10021 caactatatc ataaaaataa ttatatactg ctacatttgg atatccatca gtgtcctttt
    10081 taatatcatc taagctacct tcttcaacaa agtcatttaa cttttcatct atataatcat
    10141 catcataaag ctttaaaact aatttatctt ccattatctc ttttttttct actccattat
    10201 aggttgtaaa ttctttgttt atatccttat tattaaaagt aaacattcca tttgttattt
    10261 gaattttatg tatactttta acacccttaa ttttttctat attctctaca tcactatccg
    10321 tgtattttga tatttgcggg tctgcattta ctccaaattg cattgttata ttatatctat
    10381 tttgcatatc ttcaatttta ctgttgacct cttcttgcat acttgagaat gtagtaatga
    10441 atatatatcc tcctaaagca atagacaaca tagatatagc agctctaacc ttatttcttc
    10501 caatattttt aagtcccata aatccataaa atccaaatat tttgcttact agtctgtcta
    10561 ttccaagtct acttctgtga tttttaattt tgtctgtatt tattattgat tcaataggtg
    10621 atatcttgcc gattttaaaa gttggtataa tacaagatat aaaaacagaa attattccaa
    10681 ccaatatagc ttttataact atttcattat ttatatataa tttagggtta agaccagctt
    10741 cactgtataa aacttcgtta taattactaa ttcctatata tgaatatgta accccagcta
    10801 ctaacccaag tagtatacct attattgtta taattaatgc ttgatagatt atcatacacc
    10861 ttacgtttct tttctttgca cccatcagtc taaacagtcc catttctttt actgattgat
    10921 ttagtgtaat attgaaaata ttaaatatga aaattataga tactgtagtt attaaaacat
    10981 ttcttttaaa aacattcgga tcattcttat catcttctac ataacgaagt tcccttacaa
    11041 gaggtacatt tggtagaaaa cttttttgtc ctaacttata ttttttcata agataatcac
    11101 attcaccttc aatatttctc catccacttt taaaacttaa tatactatta taactaatcg
    11161 catcatgagg tataatgtta tttgtttcat cattgccata agttactgct tcgtattgcc
    11221 aaaccacatc atagtaccct tttggttttt ccataactcc tactatttta aattctttat
    11281 ttatagaaaa taatcgatta cttccgttag tatctttata gttctttttt attgttaaat
    11341 ttaatgtaga tcctactcct tcacttaaat tcattgcttc tagtgctttt ttctctaata
    11401 ctatttcatt gttgttttta ggtagtctac cttctattat tttgttgtat ctacttttta
    11461 gatactcttc gtttgatgaa gttaatataa atgagtttcc ttttttatct gaaatacgtc
    11521 ctaaattttg taccgaatta ccctttgata ctttttcatc agtttctaat tttttagcat
    11581 catcaagtgt caaatcacta aactctacat ggtatccact gtaaagttca tgtattgcta
    11641 ctacattgtt ttcattttgt gaatctttta taacactagg tccaaatatc aacataacac
    11701 ctaacataac tcctattatt aaaagtagtg ttctaagttt atttttcttt aaatatgcta
    11761 gtgacatttt taagtaaatc cccatataat cctcctattt tactataaat tatttatcca
    11821 actcatctgt taagtagatt ggtggatatt ttaaatcttt taattcttca ttattttttg
    11881 aagcttacta ccatcttcct catgataaac ttgcagtatc accttattta catgtagtgc
    11941 ataataatca ccactataaa aatattggta ttcataacct ggataatttt tgtaaaatga
    12001 tttataaagt acccatccta attctctttt gtatactttt cttttgtaat acgctctata
    12061 tcatcacctg aaactccttg atatgtcata tatttatcta tatatttttc agcttcattc
    12121 cattttctaa aatagttgtt gtatataata aatcctatta tggctataat gcttataatt
    12181 aaagttaata aaatagcttt gtattttctc atagtgaatc ttactacttc aatttaaatt
    12241 ctttaataag ttttaattct aaagacttag ttttatcatc aaatttctta acttgaattt
    12301 ttatatctcc caaattatca acctctatat ttccttcacc tttatcaaat acttcttcaa
    12361 tttttctaaa aaattctttc tcacttattt ctacatgaat caatgatcta tatgttataa
    12421 atagccatta tgaatttatc atcttctgac agtttttcat tataagaaaa atctttaaca
    12481 cattgaattg ataatatttc ctttgtcccc ttcttgtcct tagtcattga ataatgtaaa
    12541 acttggctag gttgttcttt tgtatactcc aagtttccca aagacaaaac aaaacttcca
    12601 tctgatttat tttcagctac cctaagacca ctattttcta ttgattctga tattttcttt
    12661 gacaagtctt cttgatacta gaaatcggtt caatagtata tattttttca tttgtatctg
    12721 aaaccttatt tgtgcatcct gttattaaac ctagcgttaa gcttatagct ataaccatag
    12781 ttattgtaat ttttttatct tcatataatc ccccttatat attgaaaact cgttatttct
    12841 cataaaaaaa agacgctatc tttttctatt aaaagataac atccctatat gtttagtatc
    12901 aatatatgtt ccccaactgg taggttaatc gacccaacta tcaaaatttt gatttaatgg
    12961 tatataaata ttcaatataa atttgtcttt tttatcttca aatgataaat ctcctctata
    13021 tttttttata gaagatttga tgctttaaat ccctattcca tgtacaagtt tatctttttt
    13081 atctgttaat aataaacttt tattctttct aattgaattt attttactat tctcacattt
    13141 aattacgaaa taggactttg agatagttcc ttttatttgt atatatttat caatactttt
    13201 atcatgtatc ttatcgcaag cttctattgc attatctaag tatttgcaaa tatattagat
    13261 acatctattg gttttataaa attcactttg gaaaaattga tatcacaaat gaatttaatt
    13321 tttctttttt cacttacatc acttttttca tttattatta tatccaatat aacattacct
    13381 gtattgtaaa ttgatttaaa ttctttaatt taatctttta aactatttac atacccatca
    13441 attttccttt tttatatttg agattttcta tacaatatat atgattattt atatcatgat
    13501 aaagcttctt aactttagcg tgtgattctt gaatacttag ataatgagaa tactgcatat
    13561 ctaacttatt ttttattact tcattttgca attttaactt attttcctta attattttta
    13621 taaacactat aataatgact aagctactag ata
//
LOCUS       DAELLV010000092        13631 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.092, whole genome shotgun sequence.
ACCESSION   DAELLV010000092 DAELLV010000000
VERSION     DAELLV010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13631
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.092"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(1011..1262)
                     /locus_tag="KPD58_002065"
     CDS             complement(1011..1262)
                     /locus_tag="KPD58_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265830.1"
                     /translation="MSSYFITRGDVMKILQQFKPYKYDKEKNIEYDEKIIANQNTEVY
                     IVYPRISDEENRRRWEEFNEVAKEVAINIAMKKVETVES"
     gene            complement(1508..1663)
                     /locus_tag="KPD58_002066"
     CDS             complement(1508..1663)
                     /locus_tag="KPD58_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HBG0265831.1"
                     /translation="MALDVKIEVKIIKDVVILNITHWKKETSLLTVKIARSQGDLSYV
                     KYFANES"
     gene            2374..2598
                     /locus_tag="KPD58_002067"
     CDS             2374..2598
                     /locus_tag="KPD58_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0265832.1"
                     /translation="MDVNQRIKYIRENMNSSQKELLEKTNINTSVMNRIESGERPTRD
                     DGLIIFAKIFGLYTDYILGLSDTEKLNIGN"
     repeat_region   2885..3243
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=2950..2978
                     /rpt_unit_seq="ctttacataccacttagttaatataaaac"
     gene            complement(3897..4049)
                     /locus_tag="KPD58_002068"
     CDS             complement(3897..4049)
                     /locus_tag="KPD58_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265833.1"
                     /translation="MDNFLFNVLASLTASVVVYLISKLFKKAKSHSRTKSDLRVEFKF
                     IFKSKK"
     gene            complement(4199..5626)
                     /locus_tag="KPD58_002069"
     CDS             complement(4199..5626)
                     /locus_tag="KPD58_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp26"
                     /protein_id="HBG0265834.1"
                     /translation="MKIKKLLTIGLSLSIFIASCPISANALDKIESIKGADKYETAGI
                     IADKQNYTTAILINADSTMSDGLSASGLAGAINAPILLTKKNNIPNATLKRLEKAKKV
                     YIIGGENSIDKYTETVLKGKGIEIKRLQGSDRIKTSYNVAKEINSINKVNKVILTNAF
                     KGEPDAMSVAPVAVRDKAAIVLTDGKSVPFNTTGVESYVIGGTSSMNDKIVKDTNSTR
                     LGGTDRYDTNKKIINKFYSGVKEFYIASGTDLVYALVGSTAAKNNAIVLVDNDSNKSV
                     LKSTTKLTAIGNLSDSILQQCLNVTKNIGDSNTGREYTVEDAANAVKKLLGVKKFYYK
                     PNYSEDEDLIFLLDKNFDFSGKEYYVFAYIKQGAEGDVRYCVAKNDMSKVYTYDVNGN
                     MKVCNPDDYKNKGSITEEQAKQIALKECAKRHKVSINNLVVDMIDFRDDIYSGNVYAV
                     KINSKNSNGTFGWFFIDIETGKVYN"
     gene            5817..6638
                     /locus_tag="KPD58_002070"
     CDS             5817..6638
                     /locus_tag="KPD58_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG0265835.1"
                     /translation="MIISKKITAILVSFLIMISMVGCAPKESATEVAKKYLDSIKIKD
                     YEVAYNLLSKDSKESVSLEKFEEYQDTLFKLQTLKDYKLGEENKVEKYKYSNKEYTNL
                     VKIKETDTIKYITENKEDTANFDRYFIIEDNEYKLLWHKDFKRALSSNYVKIARSETE
                     KDEDKDYNKAAIDIKKAIEIDDKNPLAYYTQSYIYIELQRYDEALKSIDKYLEYLDKN
                     NEAYKIDASDAYNLRGVIFMSKSEFNKARESYNKALELNPDNEYAKTNLNDIKGL"
     gene            6763..8280
                     /locus_tag="KPD58_002071"
     CDS             6763..8280
                     /locus_tag="KPD58_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG0265836.1"
                     /translation="MSVAIYLRKSRADEEAEKQGEFETLSRHKSTLLKLAKEQNLDVI
                     EIKEELVSGESIIHRPKMLELLKEVEENKYDAVLVMDLDRLGRGDMKDQGIILETFKE
                     SKTKIITPRKTYDLTDEFDEEYSEFEAFMARKELKLISRRMQRGRIKSVEEGNFIGTS
                     APFGYDAVTTGRKERILVPNKDADVVRTIFDLYINEDMGCSKISKYLNNLGIKTATGA
                     NWYNSAITNIIKNKVYCGYIQWQKKDYKKSKNPNKIKTVKLRPKDEWIEAKGKHEPLI
                     SEITWKKAQNILKKNGHVSYGNQIKNPLAGIVICKNCGRPLVYRPYADHDYIICYHPG
                     CNKSSRFEFIEAAILKSLEDTVKKYQLKASDIDLDKNNKDSNIEFQKRVLKGLETELK
                     ELGKQKNKLYDLLERGIYDEDTFIERSNNISSRTEEIKDSIKTVKNRLSTVKKDNSKI
                     IEDIKTVLSLYHDSDSLGKNKLLKSVIDKAVYYKSKEQKLDSFELMVHLKLHEDQ"
     gene            8303..8587
                     /locus_tag="KPD58_002072"
     CDS             8303..8587
                     /locus_tag="KPD58_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG0265837.1"
                     /translation="MCNEISLLDILGTEANYVLDEVELKVQVGKLYEQLNKILTPRER
                     EIVQLRYGLTPYGYKTQREIAQKLDISRSYVSRIEKKALKKLEKELVQES"
     gene            complement(8684..10348)
                     /locus_tag="KPD58_002073"
     CDS             complement(8684..10348)
                     /locus_tag="KPD58_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HBG0265838.1"
                     /translation="MSKKKTPFLKKVGKRSWCIFTIILIIYSVLIYRLVDIQVLKGDK
                     YKQSVESQSVEKVELNSGRGIIYDRNNKKLTDTSKSQVLIVEKEKLNNNYKILELIKK
                     ATKMNDLDIYKAVQEQLTRPIIQIQTKNIDKSMKKELEKNGIMVEEKTMRYAKDGLLS
                     HTIGYIKEDDKSGQSGIEKSMDSVLRNSNEKYISAFKAGDAGNEKSLNILKGSVKTVD
                     NKDKDRHLKTTIDYNIQKKLEQILNKEENPTAAIISEASTGEILAMCSRPNFDQNDIS
                     KSLKGKNGEFENRVIKATYPPGSVFKMVVLFSALENGVIDENYTYNCTGKTKVGNTNE
                     ILRCNKRDGHGFQNLRQAFSNSCNPAFLDIAMKLGKEKILKSAEKLHLFEKVDIGLDE
                     EKIREAPKNISIRNLAIGQENIEFTPLQINQMTQIIANNGTFKPLYLYKSLVDNNMNT
                     IKTYKSSKKEELISPYVCTQVKEYMKSVSRIGTAKDLKDIEGGCGVKTGTAQSSLNKK
                     AIDHGWITGFYPEERPKYVITVLVEGTQKGNKSATPIFKEICESIK"
     gene            complement(10420..11667)
                     /locus_tag="KPD58_002074"
     CDS             complement(10420..11667)
                     /locus_tag="KPD58_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBG0265839.1"
                     /translation="MELNKVELLAPAGDLERLKIAITYGADAVYIGGEIFGMRSAAKN
                     FSKEDMAEGVAFAHERGKKVFVTVNIIPHNEDFLQLEDYLLELEEIGIDAVIIADPGV
                     LSVIKKVIPNMEIHLSTQANTTNYLSANFWYEHGIKRVVVARELSFDEISEIRAKTPL
                     DMDIEAFMHGAMCISYSGRCLISNYMTGRDANKGSCAQSCRWQYHLVEEKRPGEYFPI
                     YEDERGTFFFNSKDLCMIEYIPELIKSGITSLKIEGRMKTAYYVATVVRAYRMAIDEF
                     YRDPENWKFNPMWMEELKKGSHRHFTSGFYLNKPTTEDQNYQSASYVRNYDFIGIVRE
                     TEDEDGLIVVEQRNKMCVGDEIEVMGPYKETMFTKIEEMYNEEGEAIESAPHPRQIVK
                     LKLSVKVGKDYMLRKVIEEKVEE"
     gene            complement(11654..12328)
                     /locus_tag="KPD58_002075"
     CDS             complement(11654..12328)
                     /locus_tag="KPD58_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HBG0265840.1"
                     /translation="MSNIVNDLVEDYIRVTLKEKEGFLKDLEIYAEENSVPIIHKEVS
                     DLLKVLLKVQKPKRILEVGCAIGYSSIFFATIIGKDADIITVERNEKMIEKAKENIKL
                     AGFDNNITILEGDAEEKLSQVQGEFDLIFIDAAKGQYKLFFDLVIDKLKDGGLLVSDN
                     ILYKGMVAHDDFVVRRKKTIVKRMRNYLDYICNCDYLSTSLVPIGDGVALSYKESKRG
                     DVDGIK"
     gene            complement(12500..13579)
                     /gene="mltG"
                     /locus_tag="KPD58_002076"
     CDS             complement(12500..13579)
                     /gene="mltG"
                     /locus_tag="KPD58_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HBG0265841.1"
                     /translation="MNFKENRLKIAVLIIVILIILAGIFVFIQIGPYDKNNKKDVIID
                     VPSGASVGKISDILYENKLIKNELLFKLLVKVSNKAPSIKSGTYLLNQSYSNNDIISL
                     LVSGKIYQDGIKVTIPEGATSKEIIAMLVSKNLGDKATFENLIKKPQEFYDKFPYLKE
                     DGITSLEGFLYPETYYFNSKKQSEEDILSEMLKVFDSKYTDKFKKKQKELNMTLQEVM
                     EMASIIEKEAVLDKDRPIIASVFYNRLKVGMPLQSDATIQYIFEERKKIVTYDDLKID
                     SPYNSYKNKGLPPTPISNPGIKSIEAALYPEKTDYLYFVAKIDGGNNYSTNYQDHLKY
                     VKEYKEARDKQSKDTKATNKENTKK"
BASE COUNT         4694 a         2106 c         1533 g         5298 t
ORIGIN      
        1 agttttaaaa ttggaaacca aacaataagg gcgaaaaata cttcaaggaa aatatataaa
       61 gttctataaa ttatcaaaaa tatttcgcaa aaattgataa ttattacaaa taaattgata
      121 atattagaaa tatcgaaatt gaacaaacta ggattaaagc tcaattagaa aaatccacta
      181 atcctgaaaa tactttgtta ttctcattta ttactgttgt tttttcatta tttgctaatt
      241 tatttttcag ttcattaaat gttaaatata actctataaa cttaatttta cagttaatat
      301 gtattatagt tcttatattt atatatatta atacattttt atatcgcctc ttcaatttga
      361 gccccatcat tatataaaat taacctactt gatatcttaa tttaatatat gtacactatt
      421 catatcttaa tagtcaatat tattcatttt taatagctct ttttagatgt cagaattatt
      481 atattttgaa atttattaaa tttttgcatt aaaaaaacta gaaattaatt taagtcttga
      541 attacttata actttctacg tatctacctt tatattctta ctctgatttt atatctaata
      601 ttaattcttc atcaaagcta ttaaatatac ctctaattga gttaattatt tcacttactt
      661 cacctttaat ttttatatta acttcgacca tttcaaatct catcttttta tcttataatt
      721 tctataagtt tttttattta ttgtgttgct actttgacca gcactttttt atttaatagt
      781 ctaaatgata ttttttacca gttttaatat ccttaaatac tatatttact aaaaacttat
      841 aatttctttt aatacatagt taatatattc ttattactaa taacaatact caacaatttt
      901 atcgcctttt ttcaaatatg atttaacttt tttattccta attatttaaa aatagttgtc
      961 caattttctt ctatgagaaa gtgctaagta ttagcaactt ctctattctc ttaactttct
     1021 actgtctcaa ctttcttcat agctatatta atagctactt ctttagctac ttcattgaac
     1081 tcttcccatc ttctcctatt ttcttcatca gatatacgtg gatatactat ataaacttct
     1141 gtgttttgat tagctataat tttttcatca tattctatat ttttttcttt atcatatttg
     1201 tatggtttaa attgttgtag tattttcata acatcacccc ttgttataaa atatgaactc
     1261 ataaacttgt cttatactta tttaattgtt acaattttta cttaaaatca ctttatattc
     1321 tatattttaa agctaaatcc tttacaaaag taacatattc ttctataagt ttcttatcct
     1381 cttacatagt atctagtttg tttaacttat ctcttttaaa cttacatacg ccttcttctg
     1441 ccattcgtct tttattaatt gttaaccttg tttcaagaga cacaccaaaa ctcttgttaa
     1501 gaagctctta gctctcatta gcaaaatatt ttacataact taaatcacct tgacttctag
     1561 ctattttaac tgttaataga cttgtttctt ttttccaatg tgttatattt aatataacaa
     1621 catcttttat tattttaact tctattttta catctaatgc catattattg ctttattaat
     1681 ttgtatcctt aaatcttaaa ttttctatta atacatcttc tatacagctt agtttctctc
     1741 gcttaatctt gaaatatatt ttctctaaat tgtcaaattg ttcccaagtt ttatcaatat
     1801 ctaatatttt gcagtgtcta tttgcacatc tttcgatcca aaggtataat tgactagaat
     1861 gttttgaaat caactgacta ttaatagatt agttcttaaa ttctttcaaa tactcgaatt
     1921 gtaataaaac ataatttaca ttctctttat ttctattgaa gttagtttat atattgttta
     1981 catcattttc atatatatct gctagttgct atgcatttaa aattctctta ttatttcttt
     2041 atattacttg taaattattc atcctcatca tcctcttaat taaatttcta tcttagaaat
     2101 ttaatttgta aaaaaatttt ctatatcata atcaagtatc ttagatagtt tataggtaac
     2161 atatcaactc taattgaata ttcccctttc ctgtacttaa aataaattga tgcattttta
     2221 aaacctaact catttgcaac actttgtaag tataaatttt atgtatgtct tcttttttat
     2281 aaaatcatag tgtatctttt tcatatattc acattaattg ctatatttca ataacaatat
     2341 ttccaaaaca gaaacttttc gagataatta tgtatggatg taaatcaaag gattaaatat
     2401 attagggaaa atatgaatag ttctcaaaaa gaattgcttg aaaaaacaaa tataaataca
     2461 agtgttatga atagaataga gtctggtgaa agaccaacaa gagacgatgg gttaataata
     2521 tttgctaaaa tttttggttt atatactgat tatattcttg gtttatcaga tacagaaaaa
     2581 ctaaatattg gcaattaaag ctgaattaat tgtattatat gtgtgatgaa tataaataga
     2641 gaattttttg aaaagcttga aataacttag aaaaaataag aggttcactc aagaacaagt
     2701 tgctaaagat taaaaaacaa caaaggtatc tactaaaaga tatgaaaata gtttaagaga
     2761 gttgagtctg gaaatattta tgtgtattct ttaaataaaa atttattttt taaatgataa
     2821 ttaaattttt actgatatgg ctatgctttt aaaagtccta accttatttt gatattatat
     2881 ctgtctttac ataccactta attgatataa aacagtataa aaattagtta cagatgtatc
     2941 ttttttgttc tttacatacc acttagttaa tataaaacta ttaattttcc tatgcttgta
     3001 ctcatactca aaaagcttta cataccactt agttaatata aaacgattgt tattttacga
     3061 aaggtacggt cgtaatttac gactttacat accacttagt taatataaaa caagctagcg
     3121 gggatagaca atataaatat caacacttct ttacatacca cttagttaat ataaaacaaa
     3181 cggaatatat atatacagag cttagagggg aacactttac ataccactta gttaatataa
     3241 aaccccaaaa taaattgagc atttccaaga cttacatcta tacaattctc tcaaatttgc
     3301 agtgaaccac gagtagtgca aatgataata tttatcatat accctcaatg ccttatgttt
     3361 caactgttaa gccctatttt atcaaaaata tcgctcactg caaaactatt tataatatta
     3421 tgaatatatt atttttaaat atctgtacca atttgtagta taataaaatc aagaaaataa
     3481 tttactccta taaagagtga ttatttttaa gtgaaaatat tatttttttg aatcattctt
     3541 attggcgttt gggagatttt tattttatca taaatataaa ctgatataac accagctgac
     3601 atgcttgctg ctttcgatta ttattcttgc tacaactatt ataacatata aaacttactt
     3661 atttttacca aatattttat gtaaacaata aatattaact tatgaaatat ctgctcattt
     3721 acagatattt aataagttaa tagctctata ataaaatttt tatcattgtt tataaaaaat
     3781 tttactttgc atttttttat aaacaacagt tttatttata atgttttgtg gtataataaa
     3841 agcaagaaga actacaatct attttgcgtt agagtggagt tcatcataaa cagagtttat
     3901 tttttggatt taaatataaa tttaaattca actcgtaagt cactctttgt gcgagagtgg
     3961 ctttttgctt ttttgaatag tttactgatt aagtaaacta ccacactagc tgttaaacta
     4021 gctaaaacat taaacaaaaa gttatccatg aactcacctc ccttcgaatc gttgggagga
     4081 taatcttttg tacatgaact ccactctata gattgtaagt taaatcttct tgctaaaaat
     4141 attataacat attatggtaa attatataat taaaaacact tgtttaaaag ttctaatttt
     4201 aattataaac tttcccagtt tctatatcta taaaaaacca accaaaagta ccattactat
     4261 tttttgaatt gattttaaca gcatagacat ttccactata tatatcatct ctaaaatcta
     4321 tcatatcaac tactaagtta ttgatactga ctttatgtct cttagcacat tctttcaatg
     4381 ctatctgttt tgcttgttcc tctgttatac ttcctttatt tttataatca tctggattac
     4441 atactttcat attaccatta acatcatatg tataaacttt gctcatatca tttttagcta
     4501 cacagtatct aacatcaccc tctgcaccct gtttaatgta agcaaataca tagtactctt
     4561 ttcctgaaaa atcaaaattt ttatctagta gaaatattaa atcttcatct tctgaataat
     4621 taggtttgta ataaaatttc ttcacaccta atagtttttt cactgcatta gcagcatctt
     4681 ctactgtata ttctcttcca gtattagaat cgcctatatt ttttgttaca tttaaacact
     4741 gctgtaatat actatcactc aaatttccta tagcagttaa tttagttgta cttttcaata
     4801 cacttttatt actatcatta tcaactaaca caatagcatt atttttagca gcagtagaac
     4861 caacaagagc atacactaaa tcagtaccac ttgctatata aaattctttt actccactat
     4921 aaaatttatt tattatcttt ttgttagtat catatctatc tgtcccacct aatctagttg
     4981 aattagtatc cttaactatt ttgtcattca ttgatgaagt accacctatt acataacttt
     5041 ctacaccagt tgtattaaat ggaacacttt ttccatcagt taatactata gctgctttat
     5101 ctctaactgc tacaggtgca acactcattg catcaggctc tcctttaaat gcattagtca
     5161 atatgacttt atttactttg ttaattgagt ttatttcttt tgcgacatta tagcttgtct
     5221 taattctatc actaccttga agtcttttaa tctctattcc tttgcctttt agaacagttt
     5281 cagtatattt atcaatagag ttttctcctc caattatata tacttttttt gccttttcta
     5341 atcttttaag tgtagcattt ggtatgttat ttttcttagt caaaagaatt ggagcgttta
     5401 tagctcctgc aagtccactt gcacttaatc catcagacat tgtactgtct gcattaatta
     5461 atatagctgt agtatagttt tgtttgtctg caattatacc agcagtttcg tatttatctg
     5521 cacctttaat tgattcaatt ttatcaagag catttgcaga tatagggcaa ctagcaataa
     5581 atatagataa agataaccct attgttaata attttttaat tttcataata tctccttctc
     5641 ttaaatattt tcacctaaat tatataatac ttttcaggaa aatgttacca taaatttaaa
     5701 aagttattat tacttaatat aatattaatg aaaaatatca ctcctaaatt aatgactttg
     5761 gtagaatatg gtgataaaat aaaattaatg agagacaata taagggggat tatattatga
     5821 taatcagtaa aaaaataaca gcaatattag tatcattttt aattatgata tcaatggttg
     5881 gatgtgctcc aaaagaaagt gctacagaag tagctaaaaa gtaccttgat agtataaaaa
     5941 tcaaagacta cgaagtagca tataatttat taagtaaaga cagtaaggaa agcgtcagtc
     6001 tagaaaaatt tgaagaatat caagatactt tatttaaact tcaaacccta aaagattata
     6061 aacttggtga agaaaataaa gtagagaaat acaaatattc taataaagag tacacaaatc
     6121 ttgttaaaat aaaagaaact gatactataa agtatatcac tgaaaataaa gaagatactg
     6181 caaattttga tagatatttt ataatcgaag ataatgaata taaattatta tggcataaag
     6241 actttaaaag agctcttagc tctaattatg tcaaaatcgc taggtcagaa acagaaaaag
     6301 atgaagataa ggattataat aaagctgcta ttgatatcaa aaaagctata gaaattgatg
     6361 ataaaaatcc acttgcatat tatacacaat catatattta tatagagtta caacgatatg
     6421 atgaggcttt aaagtcaatt gataaatact tagaatatct agataaaaat aatgaagctt
     6481 ataaaataga cgctagtgat gcttacaatt taagaggtgt tatttttatg agtaaatcag
     6541 aatttaataa agctagagaa tcttacaata aggcgcttga acttaacccg gataatgaat
     6601 atgcaaaaac taatctaaat gatattaaag gattataagg cattaatatt atagaaaata
     6661 agtatgttga aagttaaatt aacttttgac atgcttattt tctatatttt aaactctatt
     6721 gatttactat ataatagtgc ataaggagtg tgattactta ttatgagtgt tgccatatac
     6781 ttaagaaaat cccgtgctga tgaagaggca gaaaaacaag gcgaatttga aactttaagt
     6841 agacataaat caaccctact aaaactagca aaagaacaaa acttagatgt aattgagata
     6901 aaagaagaat tagtttctgg tgaaagtata atacatagac ccaaaatgct tgaacttcta
     6961 aaagaagttg aagaaaataa atatgatgca gtcttagtaa tggacttaga cagacttggt
     7021 cgtggagaca tgaaagacca aggtatcata ttagaaacat tcaaagaaag taagaccaaa
     7081 attatcacac ctagaaaaac atacgaccta acagatgaat ttgatgagga gtattcagaa
     7141 tttgaagcat ttatggcaag aaaagaatta aaacttatat caagaaggat gcaaagaggt
     7201 agaataaaat cagttgaaga agggaatttc ataggaacct ctgctccatt tggatatgat
     7261 gctgtaacaa ctggtagaaa agaaagaata cttgtaccaa ataaggatgc tgatgtagtt
     7321 aggactatct ttgacttata tattaatgaa gatatgggtt gtagtaaaat atcaaaatac
     7381 ctaaataact taggcattaa aactgctaca ggtgctaatt ggtacaattc tgcaattaca
     7441 aatataataa aaaataaggt atattgtggc tacattcagt ggcaaaaaaa ggactacaaa
     7501 aaatctaaaa atccaaataa aataaaaaca gttaaactaa gacctaaaga tgaatggatt
     7561 gaggcaaaag gaaagcatga acctttaata agtgaaataa catggaaaaa agctcaaaat
     7621 atactcaaaa aaaatggtca tgtcagctat ggtaatcaaa taaaaaatcc acttgcagga
     7681 atagtgattt gcaaaaattg tggtagacca ttagtatata gaccatatgc agaccacgac
     7741 tatataattt gttatcatcc tggttgcaat aaaagttctc gatttgaatt tattgaagct
     7801 gccattttaa aatctttaga agacactgta aaaaaatatc aattaaaagc atctgatata
     7861 gacttagata aaaataataa agatagtaat atagaatttc aaaaacgagt cttaaagggc
     7921 ttagaaactg aattaaaaga attaggcaag caaaaaaata agttatatga tttactagaa
     7981 cgaggaatat atgatgaaga tacatttatt gaaaggtcca acaatataag ttcaagaact
     8041 gaagaaatta aagattcaat taagacagta aaaaatagat taagtactgt taaaaaagac
     8101 aattctaaaa taatagaaga tattaaaact gtgttatcac tttaccatga ttcagattcc
     8161 cttggaaaaa ataaactcct aaaatctgta attgataaag cagtttatta taagtctaaa
     8221 gaacaaaaac tagattcttt tgaattaatg gtccatttga aattacatga agaccagtaa
     8281 taccaataac atataaagag tagtgtgtaa tgaaataagt cttttagata tacttggtac
     8341 agaggctaac tatgttcttg atgaagttga actaaaagtt caagttggaa aactttacga
     8401 acaactaaac aaaattctta ctcctagaga aagagaaatt gtccagttaa gatatgggtt
     8461 aactccttat ggctataaga cacaaagaga aattgcacaa aaactcgata tatcaaggtc
     8521 atatgtatct agaatcgaaa aaaaagctct taaaaaacta gaaaaagagc ttgttcaaga
     8581 aagttaatac tatattttaa aaggatatat cgtacatcat tagacaaaat tttctatcta
     8641 aaatcaaagt tacgatatat ccttaaattt tatagtacat aatttatttt atactttcac
     8701 atatttcctt aaatataggt gttgcagatt tatttccttt ttgagttcct tctactaaca
     8761 cagttattac atacttaggt ctttcttcag gataaaatcc tgttatccat ccatggtcta
     8821 tagctttttt atttaaacta ctttgagctg taccagtttt aactccacaa ccaccttcaa
     8881 tatcttttag gtctttagct gtaccaatcc tagatacaga cttcatatat tctttaactt
     8941 gtgtacacac ataaggagaa ataagttctt ctttttttga agacttataa gtttttattg
     9001 tattcatatt attgtccaca agacttttat ataagtatag tggtttgaat gttccattat
     9061 tagctattat ttgagtcatt tgatttattt gaagtggtgt aaattctata ttttcttgac
     9121 ctatagctaa atttcttatt gatatgtttt ttggagcttc tctaattttt tcttcatcaa
     9181 gaccaatatc aaccttttca aataaatgta atttttcagc agatttgagt atcttttctt
     9241 ttccaagttt catagctata tctaaaaatg caggattgca tgaattagaa aaagcttgtc
     9301 ttaagttttg gaaaccatga ccatctcttt tattacatcg cagtatttca ttagtattcc
     9361 caactttagt cttacctgta cagttgtaag tatagttctc atctataaca ccattttcaa
     9421 gtgcagaaaa taaaactacc atcttaaata cagaccctgg tggatatgtc gcttttataa
     9481 ctctattttc aaattctcca ttttttcctt ttaaactttt tgatatgtca ttttggtcaa
     9541 aattaggtct agagcacatt gcaagaattt cacctgtaga tgcttccgag attattgctg
     9601 ctgtaggatt ttcttcttta tttagtattt gttctaattt tttttgaatg ttataatcaa
     9661 tagtagtttt tagatgtcta tctttatcct tattatcaac agtttttaca cttcccttta
     9721 gtatgtttag agatttttca tttcctgcat ctccagcttt aaaagcactt atatactttt
     9781 catttgaatt tctaagtaca ctatccatac tcttttcaat tcctgattga cctgatttat
     9841 catcttcttt tatatatcct attgtatgtg ataataaacc atcttttgcg tacctcatag
     9901 ttttttcttc taccattatt ccatttttct caagttcttt tttcatactc ttatcgatat
     9961 ttttagtctg aatctgtatt atagggcgtg tcaattgctc ttgtacagct ttatatatat
    10021 ctaaatcatt catcttagta gctttcttaa taagctccaa aattttatag ttattattta
    10081 atttttcttt ttcaacaatt aatacctgag atttacttgt atctgtaagc ttcttgttgt
    10141 ttctatcata gattattcct cttccactat ttaactcgac tttctcaacg ctttgtgatt
    10201 caacactttg tttgtattta tcacctttta atacttgtat atcaactagc ctataaatta
    10261 aaacactata aattattaaa attatagtaa atatacacca gcttctcttt cccacttttt
    10321 tcaaaaatgg agtcttcttt tttgacatga aatcacccct tagataattt cattattgac
    10381 aatttctaag gggctaatac tatttttatc aattttttat tattcttcaa ccttttcttc
    10441 tataactttt ctaagcatat aatctttacc aacttttact gatagcttta acttcactat
    10501 ttgtctagga tgaggagctg attctatagc ttctccttct tcattgtaca tttcttcaat
    10561 tttagtaaac atagtttctt tatatggtcc cataacttct atttcatcac caacacacat
    10621 tttatttctt tgctcaacaa caataagacc atcttcatct tctgtttctc taactatacc
    10681 tataaaatca tagtttctaa catatgatgc tgattgataa ttttggtctt ctgttgttgg
    10741 tttattcaag taaaaacctg atgtaaaatg tctatgacta ccttttttta gttcttccat
    10801 ccacattgga ttgaacttcc agttttctgg gtctctgtaa aattcatcta ttgccattct
    10861 gtatgctctt acaacagtag ctacatagta tgcagtcttc attctacctt ctatttttaa
    10921 acttgttata ccagatttga ttaactctgg tatatactct atcatacata aatcttttga
    10981 gttaaagaag aatgtacccc tttcatcttc gtatattgga aaatactcac caggtctttt
    11041 ttcttccacc aaatgatatt gccatctaca agattgagca caagaacctt tgtttgcatc
    11101 tcttccagtc atatagttgc ttattaaaca tctgccagaa taagaaatac acatagcacc
    11161 atgcataaac gcttctatat ccatatctaa aggtgttttt gctcttatct cagatatttc
    11221 atcaaaagat aattctcttg ccactacaac tctttttatt ccatgctcgt accagaagtt
    11281 tgcacttaga taattggttg tatttgcttg tgtacttaaa tgtatttcca tattcggaat
    11341 tactttttta atgacactaa gcactcctgg gtcagctatt ataactgcat ctattcctat
    11401 ctcttctagt tccaacaaat agtcttctaa ttgtagaaaa tcttcattat gaggtattat
    11461 atttactgtt acgaatactt tcttgcctct ttcatgtgca aaagctactc cctctgccat
    11521 atcttcctta ctaaaattct tcgctgcaga cctcattcca aaaatttctc cacctatata
    11581 aacggcatct gctccatatg ttatagctat tttcaatctc tccaaatcac cagctggagc
    11641 taaaagttct actttattta attccatcta catcacctct tttactttct ttataactta
    11701 gtgctacacc atcacctatt ggtaccaatg atgtgcttaa atagtcacaa ttacatatat
    11761 aatctaaata atttctcatt cttttaacta tagtcttttt tcttcttaca acaaaatcat
    11821 catgagcaac cataccttta tacaaaatat tatcagatac taaaagtccc ccatctttta
    11881 atttatctat aactaaatca aaaaatagct tatattgacc tttggctgca tctataaata
    11941 taaggtcaaa ttcaccttgg acttgactaa gtttttcttc tgcatctcct tctagtatag
    12001 ttatgttatt gtcaaatcca gctaatttta tgttttcttt tgctttttct atcatttttt
    12061 catttctctc aacagttatt atatctgcat cttttcctat aatagttgca aaaaaaatag
    12121 aagaataacc aattgcacat cctacttcta aaattctctt tggtttttga acctttaaca
    12181 agacttttaa taaatctgaa acttctttat gtattatagg aacactattt tcctctgcat
    12241 aaatttctaa atcttttaaa aatccttctt tttcttttaa tgtcactctt atataatctt
    12301 caaccaaatc attaactata ttactcataa aaacactccc taaaataata aatattcaag
    12361 ttttatatta atttattatt ttttaaattc atattgttat atatatttac actttcttta
    12421 ttataaatct aagaataaca attttcaata aaaaagccca acattattct taccctgtta
    12481 gactttttat aatcatggtt tatttttttg tattttcttt atttgttgct tttgtatctt
    12541 ttgattgttt atcccttgct tctttatatt ctttaacgta ttttaaatgg tcttgatagt
    12601 ttgtactata attatttcca ccatcaatct tagctacaaa atataaatag tctgttttct
    12661 ctggatataa tgctgcttca atagatttta taccaggatt tgatattggt gtaggaggta
    12721 atcccttatt tttataacta ttatatggtg aatcaatttt taaatcatca tatgttacaa
    12781 ttttctttct ctcttcaaat atatattgta ttgtagcatc tgattgaaga ggcataccta
    12841 ccttaagtct attataaaac acacttgcta ttataggtct gtctttgtca agaacagctt
    12901 ctttctcaat tattgatgcc atttccatta cttcttgaag agtcatattt aattcttttt
    12961 gtttcttttt aaacttgtcc gtgtatttac tgtcaaatac ttttaacatt tcgcttaaaa
    13021 tatcttcttc tgattgtttc tttgaattaa aataataagt ttctggatac aagaaccctt
    13081 ctaaagaggt tattccatct tccttcaaat atggaaattt atcataaaat tcttgaggtt
    13141 tttttattaa attttcaaaa gtagctttat ctcctaaatt tttacttact aacattgcta
    13201 taatttcttt agatgtagct ccctctggaa tagtcacttt tataccatcc tgatatattt
    13261 tacctgaaac taaaagactt attatatcat tgtttgaata cgattgatta agtaagtatg
    13321 ttcctgattt tatagacgga gctttattgc taacctttac caaaagttta aataatagtt
    13381 cattctttat taatttattt tcatataata tgtcagatat ttttccaaca gatgctccac
    13441 ttggaacgtc gataattaca tcttttttgt tatttttatc ataaggacct atttgtataa
    13501 acacaaatat acctgctaag ataatcaaaa taacaattat taatactgct attttcaatc
    13561 tattttcttt aaagttcata atataatcta ctccttatgt atatttatcc attttctatt
    13621 ataaaactag a
//
LOCUS       DAELLV010000093        13364 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.093, whole genome shotgun sequence.
ACCESSION   DAELLV010000093 DAELLV010000000
VERSION     DAELLV010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13364
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.093"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(178..1059)
                     /locus_tag="KPD58_002077"
     CDS             complement(178..1059)
                     /locus_tag="KPD58_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HBG0265842.1"
                     /translation="MKITEGYMPFKGFKTYYRIVGENTEGKKPLVLLHGGPGSTHNYF
                     EVLDKIAESGRQVIMYDQIGCGNSFVEGHPELFNADTWIEELIELRKHLGLDEIHLLG
                     QSWGGMQAIWYAIEYKPKGIKSYILSSTLSSAKLWEKEQKRRISYMSEVDQKALLDAV
                     NTGDYSSKEYNDALERFMEMYCAGEVTEDSPECLRRPKKSGSEAYIVGWGQNEFSPTG
                     TLSGYEFTDRLHEIKEPCLVTSGAIDLCSPYIAKTMYDRIPNSKWELFEYSRHMPFVE
                     ENEKYIKVLTEWLNAND"
     gene            1515..2489
                     /locus_tag="KPD58_002078"
     CDS             1515..2489
                     /locus_tag="KPD58_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265843.1"
                     /translation="MNNINSLKVIKLSLGCSLAIFIAWLLKLEYSMVAGVIVLLTVKD
                     TKRETLKGSIGKIYGFLLCTIFSYLCFNILGYNLTSFSIFIFIIIPLCFLLNIQDVIA
                     MCVVIASHYYLQGETSIKWILNEAGIFAVGTGIGVLINMYIPTNIHKIHEVQKKLQEE
                     VSIVLIDIADIIVNPKKENGYSRDLYTLNSLIDSSISETYDNINNTLLSDTRFFLEHM
                     DIIKSQRDILENLYSYVSQLNSTPPQAHILSAFIHKIGYTEFEAETGNLLLEELKRLM
                     ISMKNQPLPVDRTEFENRAILFLCLTELKQFLVNRKHAQMLRDNNFYK"
     gene            complement(2605..3666)
                     /locus_tag="KPD58_002079"
     CDS             complement(2605..3666)
                     /locus_tag="KPD58_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBG0265844.1"
                     /translation="MKKIRKLATKLLITTIVLISGMSMTVYGMTAKEVTAKTPKSYVT
                     GTNSVYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKILVAYNHIKNNTTYVDWNA
                     VEGANTAYTLVTKKGACSGMARSMKALCDAMGIESYYVHSTSNDHQWNLIRFGDGRLY
                     HVDIDANKSAGKDIIYKSLSHPLPFDKTAYPKVGAIVQTVKPEQTQKPAPPGKHTMLP
                     HQEGAKAVANFGKKGEDINEKHVMYEGVRCSVVNSSVNTITFTGSESNLDPSNLTIAV
                     IGFMNTPYGMFALAPEGKPVEYPFKYDKPFSLDFSGKNRSGAELAKYCKKNDVTIKIE
                     ITDEKANIGLGQIMYVRYE"
     gene            complement(4277..5128)
                     /locus_tag="KPD58_002080"
     CDS             complement(4277..5128)
                     /locus_tag="KPD58_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG0265845.1"
                     /translation="MSCILKLKQIYEDLTEVDKKIADYILNNTEAISKLSVSELASNS
                     KTSTASIVRFSRKMGYSGFGDLKIEIAKDLMGKENEYTYVDENYDCNIDSVINKITNK
                     NIETINQTRLLNEKDIIKEAVEQIIKAKNVYIFGVGGSALVALDLQMKLLRINKQAFT
                     SLDSHTQLMVSSNVDKEDIAIAISYSGESKEVIKSIENAKLKGCKVICITKYSDNHLS
                     KISDLKLVVPNIEKRLREGAISSRIAMLTLIDIIYISIIQENLNEAEQKLQETKRILD
                     YLNINNA"
     gene            complement(5145..6065)
                     /locus_tag="KPD58_002081"
     CDS             complement(5145..6065)
                     /locus_tag="KPD58_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HBG0265846.1"
                     /translation="MYFLGVDGGGTKTTFTLVDEELNIVGTITKGTCHYNQIGFDNLT
                     KLLITGLEEVCKDAKINVEEITYAFVGLAGYGKIKEVLYALEVATKNAYSHINYTLGN
                     DVEIALAGSLNGEKGINIIAGTGSIAQALDKDGNLHRCGGWGYVLGDEGSAYYIGMAT
                     LKMFTMQSDGRCSKTKLYDLIKRHLNIENDYDIIKYVNDEIQGDRIEIAKFATICLKA
                     VIEGDNTASKIFDDAAYELSRLIIGLEHHFEQGTKIKVSYSGGVFKSGDLILEPLKKY
                     LNKTRFGIVRPVLTPDLGACLLAKKKYYSK"
     gene            complement(6175..7089)
                     /gene="murQ"
                     /locus_tag="KPD58_002082"
     CDS             complement(6175..7089)
                     /gene="murQ"
                     /locus_tag="KPD58_002082"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBG0265847.1"
                     /translation="MLIKTLENLVTEGRNKNTLQIDKEDTLGIIELINNEDKTVAYAV
                     EEQKESIAKAVNIIVDRMKQGGRLFYIGAGTSGRIGILDATECPPTYGVDFELVQAII
                     AGGNQAIFKAIEGAEDDKELGKQDIIDRGVTSKDVICGIAASGRTPYVIGAMEYAKEL
                     GCAVLSITMNPNSEMSKKADLPINIIVGAEVIMGSTRMKSGTAQKMVCNMLTTASMVK
                     MGKVYSNLMVDVKTSNEKLVERAKRIIMIATNVKYDVAEKFLEEADNSVKLAIFMIKS
                     GLDKDSAKSILDRQEGYISEALKSIEKL"
     gene            complement(7188..8285)
                     /locus_tag="KPD58_002083"
     CDS             complement(7188..8285)
                     /locus_tag="KPD58_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBG0265848.1"
                     /translation="MGRLGISIYPEKTTSKEIYDYIDKAAENGFTRIFSCLLSVKDTK
                     ENIIKKFKSINEYAKFKGFEVILDVNPRVFGELEISYKDLNFFKKTKADGIRLDMGFT
                     GSEEALMTFNPQNLKIEINMSNNTNYIDTIMDYQPNKENLIGCHNFYPHRYSGLNLEH
                     FIKCTERFNKYGLRTAVFVTSQNETTFGPWPVTDGLPTIEMHRNLPIDIQVKHIIAMN
                     NINDIIISNCYPTYEELKVLGSMRKDMVTFDVAIEENIREVEKKILFEEIHFNRGDIS
                     DSLIRSTQSRVKYKGYKFELFNTPKIIKRGDIVIESSEYGHYAGELQIALTDMKNSGK
                     SNVVGYIRKEELFILDYIKPWQKFNFKPSTK"
     gene            complement(8518..9864)
                     /locus_tag="KPD58_002084"
     CDS             complement(8518..9864)
                     /locus_tag="KPD58_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0265849.1"
                     /translation="MLDKFTEFLDTKLSTSMARLAEQRHLRAVRDGIVASLPLIIVGS
                     FFLILAFPPLPDSWGVTQYLKENAVTILLPYRMTFFIISVYSTFGIGYSLAKSYKLDG
                     LTGGLLATATFLLTIVPTAIEGLTPDLLKIVEKSPELLAWYNSIPQGWQMPMANLGGG
                     SMFVAIVTSIIGVEIYRFTDKSKFKITMPEAVPASVTRSFEALTPAALIIILMAIVTY
                     FLGFDWHGFIAKLVTPLVSATDTLPSVIILIFLITFFWSFGIHGVSIIGSLARPLWIQ
                     LLDANTASYAAGQAVPGIAAEPFFQWFIWIGGSGATIGLALLLAFRAKSAYGKSLGKA
                     TVIPAIFNINEPIIFGAPIVLNPILIIPFVVAPIVMATVSWFAMSANLVSRVVVTAPW
                     TLPGPVGAYLATGGDWRASVLNLLCIAISIIIYYPFVKMYDKRLLHEEKVEEQEAY"
     gene            complement(9953..10261)
                     /locus_tag="KPD58_002085"
     CDS             complement(9953..10261)
                     /locus_tag="KPD58_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0265850.1"
                     /translation="MRVLFVCSSGMSSAIAVNALQKEGAKNGIDIDVLAVGTQEFENE
                     VKNGWDIAMVAPQVRHRFDYLKAFADEVSVPCALIQAQAYSPLGGPKLLKQVQELLSK
                     "
     gene            complement(10523..10921)
                     /locus_tag="KPD58_002086"
     CDS             complement(10523..10921)
                     /locus_tag="KPD58_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265851.1"
                     /translation="MSNNKKKKRKKNNNYKNAKSTKSNKNTKKQMEKNQRTDYNIPTI
                     SFKSGLILIVSSLVSTVVFPLLLSIFGIDNRFGVVIGNSLITSFAVAYTRYFVETKRG
                     FSLGFFRLYILFALSFAIIGYFWIYKGIHI"
     gene            complement(10923..12083)
                     /locus_tag="KPD58_002087"
     CDS             complement(10923..12083)
                     /locus_tag="KPD58_002087"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HBG0265852.1"
                     /translation="MITMYSVGLMSGTSLDGVDAVLAEISGNGRNTKVKQIEFITLEI
                     PKDIKDEIRKCCIEEESSVDLICSLNFKLGYLFSKAVKSVCHKANFHIANLDFIASHG
                     QTIFHIPRSYNNFVSSTLQIGEPAVIAYETNTKVISNFRVMDIAAGGEGAPLVPYSEF
                     LLYSDNNKNLALQNIGGIGNITIIPKSCNIDDVFAFDTGPGNMIIDGVCQRLFNRKYD
                     KNGYFASKGKINEEMLKDLMSHKYLSQAPPKSTGREVFGQVYLDNMLNKYKHVDKYDL
                     IATVTMFTAKAIYYNYKNFILPKVNVDTLLIGGGGAHNLTLIGYIKELLLEVEVLTQD
                     EYGYSSDAKEALAFVILGNETLNNSFSNVISATGAKNKVILGNITPKPFGGK"
     gene            complement(12514..>13364)
                     /locus_tag="KPD58_002088"
     CDS             complement(12514..>13364)
                     /locus_tag="KPD58_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0265853.1"
                     /translation="DYINFRNSYTNLYNCIENSGEIDSYLVDGQSFEENLIKDIIENL
                     KIKNNSDIETYKQSLKELDEYIAKWVHEIKIPISSLSIITDRLSSIEDSLDIKNQVAK
                     INFLVNSILYSSRSNTMFEDVFINKFNLEKLVKMSIKNNSFLLIKNNVEVSLNELEND
                     VYTDSKCMSYVLDQIINNAIKYSKEVGKIEFNSKKLENGVVLSIKDFGIGINEEDISR
                     VFDKGFTGKNGRNQLYKSTGMGMYFVKKMIDSLGHEIEVCSENGSYTIFNIYFYDISD
                     YLSLDS"
BASE COUNT         4282 a         2349 c         1467 g         5266 t
ORIGIN      
        1 tacaattaga ttagaatcta acgctagtaa taaaaaatta ctttcccaaa atagatataa
       61 gcctcatttt atcgttcaaa taatgaaaat atatttgatt tcatgcaaaa agagtgccta
      121 atgaactaaa aagttcattt tgagacactc atttttttat ataaatttat atagttttta
      181 atcgttcgca ttcaaccact cagttaagac ttttatatat ttctcatttt cttcaacgaa
      241 tggcatatgt cttgaatact caaataattc ccacttacta ttaggtattc tatcatacat
      301 agtctttgca atatatggtg aacataaatc tattgctcca ctagttacta gacaaggctc
      361 ttttatttca tgtaatctat ctgtaaattc atatccagaa agagttcctg taggagaaaa
      421 ttcattttgt ccccatccta caatatatgc ttcagaacct gattttttag gtcttctcag
      481 gcactctgga gaatcttctg tcacttcacc tgcacaatac atttccataa atctttctaa
      541 tgcatcatta tattctttgc tagagtaatc tccagtattt actgcatcaa gtaatgcttt
      601 ttggtcaacc tcactcatat atgatattct tcttttttgt tctttctccc aaagttttgc
      661 agaagaaaga gtagatgaaa ggatgtaaga cttaatacct tttggtttat attcaatagc
      721 ataccaaata gcctgcatac ctccccaaga ttgacctaac agatgaattt catcaagtcc
      781 tagatgtttt cttagttcaa taagctcctc tatccaagta tctgcattaa atagctcagg
      841 atgcccttct acaaatgaat ttccacaacc aatttggtca tacattatta cttgtctacc
      901 actttcagct atcttatcca atacctcaaa atagttgtgt gtagaacctg gacctccatg
      961 aagtaatact aatggcttct tcccttccgt attttctcct actatacggt aatacgtttt
     1021 aaaaccttta aaaggcatat atccttcagt aattttcatg tttatctcct ccaatttgta
     1081 attataaaca ataatcaagt tcattaacaa tactatttaa aacctcttat acatatttga
     1141 ttatgtattt taatctttat acctactttt ataaatagta taacataaaa gttaaaaatc
     1201 tctatactaa accaattata aaattatatt aactatataa ttttattttt taataatagt
     1261 atatacatat ttgattattt tcatttgcta tttaaaatca attttaagtt ttcttcttat
     1321 aataaacttc ctctttgtgg tctcatgtta gtctctaaat ttaaaatttt acaaaaaatt
     1381 ttcataaaat aaaattatat tgattgtatc taaacaaata gggcaataaa ataattaagt
     1441 atagttactt acctttcaag caatattagt tttatgttag aattttatta tattgattta
     1501 caggaggttt gcatttgaat aatattaatt cacttaaagt aataaaatta tctcttggat
     1561 gttctcttgc tatatttatt gcttggctat taaaattaga atactcaatg gttgctggag
     1621 ttatagtact acttacagta aaggacacta aaagagaaac tcttaaaggc tccattggaa
     1681 agatttatgg ttttctttta tgtactattt tttcatattt atgttttaat attttaggat
     1741 ataatttgac ttcattttca atatttatat ttataattat accactttgt tttttgttaa
     1801 atattcaaga tgttattgct atgtgtgtag tcatagcatc ccattactac cttcaaggag
     1861 aaacttctat caaatggatt ttaaatgaag ctggaatatt tgcagtagga actggaatag
     1921 gtgtcttaat aaatatgtat attccaacaa atatacataa aatccatgaa gttcaaaaaa
     1981 agttacagga agaagtgagt attgtactta tagacattgc agatattatt gtaaatccta
     2041 aaaaagaaaa tggatatagt agagatttat atacattaaa ttcacttatt gacagcagta
     2101 tttctgaaac ttacgataat attaataata ctttattaag tgatactaga ttcttcttag
     2161 agcatatgga tattataaaa agccaacggg atatcttaga aaatttgtat agttatgtat
     2221 ctcaactaaa ttctacacca ccacaagctc atattctatc tgcttttata cataagatag
     2281 gttatactga atttgaagct gaaacaggca atcttcttct tgaagaatta aagagactaa
     2341 tgattagtat gaaaaaccag cctctacctg ttgacagaac agagtttgaa aatagagcta
     2401 ttttattcct atgcttgact gagttaaaac agtttttagt taatcgtaaa catgctcaga
     2461 tgcttagaga taataatttt tataaataaa aaggggagaa atctagcagt tgacctgact
     2521 acaaaatatc agatataata ttaaatgcgc ccctagtaag agggcgcaat gtttataact
     2581 tattctactg tcatgattaa atcattattc atatcgaaca tacattattt gaccaagtcc
     2641 aatattagct ttttcatcag tgatttctat tttaatagtc acatcatttt ttttacagta
     2701 cttcgctaat tctgcaccag aacgattttt accactaaaa tctaaagaaa atggcttatc
     2761 atatttaaaa ggatattcaa caggtttacc ctctggtgct aatgcaaaca tcccatatgg
     2821 tgtgttcata aatcctatta cagcaatcgt aagatttgat gggtctaaat ttgattcact
     2881 acctgtaaat gtaattgtat ttactgatga attaactaca ctacatctaa caccttcata
     2941 cattacatgt ttttcattta tatcttctcc tttttttcca aaatttgcaa cagcctttgc
     3001 cccttcttga tgtggtaaca tagtgtgctt cccaggtggt gctggtttct gtgtctgttc
     3061 tggttttacc gtctgtacta ttgcaccaac ttttggatat gctgtcttat caaatggtaa
     3121 tgggtggcta agactcttgt atatgatatc ctttcctgct gatttattag catcaatatc
     3181 tacatgatac aaacgcccat caccaaaacg gataagattc cactggtggt catttgatgt
     3241 gctgtgtaca taataagatt caattcccat agcatcacac aaagctttca tgctcctagc
     3301 cattcctgag catgcacctt ttttagtaac caatgtatag gcagtatttg ctccctctac
     3361 tgcattccag tcaacatatg ttgtgttatt tttaatgtga ttgtaggcaa ctaaaatctt
     3421 agcatctgtg gtcatattct ttgtgatttt cttgttcata aaatctgctg ttgcttgtgc
     3481 tactgagttt agttgggctt gtgacagctt tggaccatat acgctattgg ttccagtgac
     3541 atagcttttt ggtgtttttg ctgttacctc tttggctgtc ataccataaa ctgtcatact
     3601 catacctgag attaaaacta ttgttgtaat caataacttg gtagctaact tcctaatttt
     3661 cttcatactt aatattctcc tttataaaac attgttactt cctattctct accattaata
     3721 aaattaattt aggatttaaa aaggtggatt gtcaacatgt atatttctcc attcatacat
     3781 tcgtgaaata tttaaatttc atggaaaatc taatagatat tttctagata atttgtatac
     3841 aatacaactc gcaaatcagg gtgtttgctg ttagtttgag aaaatgatga tgattaatac
     3901 attcggaatc tttgacctat aataaatacc attgtcttca atcattaata atggtaagag
     3961 taaagagaat tatcaataat acagttaaaa tcaactttgt atacatttca tacattctct
     4021 cccaacaaca ttatacttct tataatttgt atattcaata cgttttctgg gaaaatatag
     4081 atatatctat aaaaatataa aaaaggagtt gaacttaaaa tttgattaaa aaaaatcaat
     4141 tttaagacaa ctcatttgta ttataaaaat gctatatatt attattaagt aagtcaatat
     4201 tttagatatt tatctatttc ttattgactt acgcttatag tctaacttat ttgtttagtt
     4261 gatatataac ctagttttaa gcattattaa tatttaaata atcaagtatc ctttttgttt
     4321 cttgtagttt ctgttctgcc tcatttaagt tttcttgaat tatagaaata tatattatat
     4381 ctatcaacgt aagcattgct attcttgaag aaatagctcc ttctctaagt cttttttcta
     4441 tatttggtac tactagcttt aaatcactaa tcttactaag atgattgtca ctatatttag
     4501 ttatacaaat gaccttacat ccttttaatt ttgcattttc tatagacttt attacttctt
     4561 ttgattctcc agagtaagat attgctatag ctatatcttc tttatctaca tttgaagata
     4621 ccataagttg tgtatgacta tctaaagagg taaatgcctg tttatttatc cttaaaagtt
     4681 tcatctgtaa atctagtgca actaaagccg aaccacctac tccaaatata tatacatttt
     4741 tagcctttat tatctgttca actgcttctt ttattatatc tttttcattc aacaaccttg
     4801 tttggttgat agtctctatg tttttgtttg taattttgtt tataacacta tctatattac
     4861 aatcatagtt ttcatctaca tatgtgtatt cattttcttt gcccatcaaa tctttagcta
     4921 tctcaatttt taaatcccca aaacccgaat atcccatttt tctagaaaac ctaactatac
     4981 ttgctgtact tgtttttgag tttgacgcca attctgatac agaaagctta cttatagcct
     5041 ctgtattatt aagtatgtaa tcagctattt ttttatctac ttccgttaaa tcttcatata
     5101 tctgttttaa ttttaaaatg caactcatac tctaccacct ctacctattt actataatat
     5161 ttcttcttag caagtaaaca tgctcctaaa tctggagtca acactggtct cactatacca
     5221 aatctcgttt tattcaaata ttttttaagt ggctctaata ttaaatctcc tgacttaaac
     5281 actccacctg aataagatac ctttatcttt gtaccttgtt caaaatgatg ctctaatcct
     5341 attataagcc tacttaattc ataagctgca tcatcaaata ttttactagc agtattatca
     5401 ccttcaataa ctgcttttaa acatatcgta gcaaactttg ctatttctat tctatctcct
     5461 tgtatttcat catttacata ttttattatg tcataatcat tctctatatt aagatgtctt
     5521 tttattaaat catataattt tgtttttgaa caccttccat cagactgcat tgtgaacatt
     5581 tttaaggtag ccattcctat ataatacgct gaaccttcat ctcctaatac atagccccat
     5641 cctccacatc tgtgaagatt cccatcttta tctaaagcct gtgctattga accagtacct
     5701 gcaattatat ttataccttt ttccccattt aaagaacctg ctaatgctat ttctacatca
     5761 ttacctagag tataatttat atgactatat gcattttttg ttgctacttc taatgcatac
     5821 aatacttctt ttatttttcc atatccagca agtccaacaa atgcatacgt tatttcttct
     5881 acatttatct tagcatcttt acaaacttct tccaacccag ttattaataa tttcgttaga
     5941 ttatcaaatc ctatttgatt ataatgacag gttcctttgg ttatggttcc tactatattt
     6001 aattcttcat ctactaatgt aaatgttgtt ttcgttcctc caccatctac acctaaaaaa
     6061 tacatactat cctcctacaa tatctttaat aattctttca atacttaata tctaaaaata
     6121 accttccaat gctgtttttt taatattgaa aggttatcag tactattatt tattttacaa
     6181 tttttctatt gattttaaag cttcacttat ataaccttct tgtctatcta ggatactctt
     6241 tgcactatcc ttatctaaac cactttttat cataaatata gccaatttaa cgctattatc
     6301 tgcttcttct aagaattttt ctgcaacatc atattttaca tttgtagcta tcattattat
     6361 tctttttgct ctttcaacca acttttcatt tgatgtctta acatctacca tcaagtttga
     6421 gtatactttt cccatcttaa ccatagaagc tgttgttaac atattacata ccattttttg
     6481 agctgtacca cttttcattc tagttgagcc cattattact tctgccccta ctataatgtt
     6541 tattggtaaa tccgcttttt tactcatttc actattcgga ttcatagtta ttgatagtac
     6601 tgcacacccc aattccttag catactccat tgcacctata acataaggag tccttcctga
     6661 tgcagctata ccacatataa catccttaga tgtaactcct ctatctatta tatcttgctt
     6721 tccaagttct ttatcatctt cagcaccttc tattgcttta aatattgctt gatttccacc
     6781 agctattata gcttgaacaa gctcaaaatc cactccgtaa gttggaggac attctgtggc
     6841 atctagtatt cctatcctac cactggtacc tgctcctata tagaataatc tacctccctg
     6901 tttcattcta tctactatta tattaacagc ttttgctata ctctcctttt gttcttcaac
     6961 agcataggct acagttttat cttcgttatt tattaattct attatgccta atgtatcttc
     7021 tttatctatt tgcaatgtat tcttatttct accttctgta actaaatttt ctaaagtttt
     7081 tattaacata tcatttcctc attttcttaa tattaaattt ttaaacacat tttaattcca
     7141 actttcataa ttatattttt ataataaatt aatattttca taataaatta ttttgttgag
     7201 ggtttaaaat taaacttttg ccatggttta atataatcta atataaataa ttcttctttt
     7261 cttatatatc ctactacatt cgatttacca ctgtttttca tatcagtcaa ggctatttgt
     7321 aactcccctg cataatgacc atattcacta ctttcaatta ctatatctcc tctttttatt
     7381 attttaggag tattaaatag ttcaaatttg tatcctttat atttaacacg agattgagta
     7441 gaccttatca gactgtcaga tatgtcccct ctattaaaat gtatttcttc aaagagtatc
     7501 ttcttctcaa cttctcgtat attttcctct attgctacat caaatgtaac catatctttt
     7561 ctcatagaac ctaatacctt tagctcctca tatgttggat aacaatttga tattattata
     7621 tcatttatat tattcatagc tattatatgc tttacttgaa tatctatagg tagatttctg
     7681 tgcatctcaa ttgttggaag cccatcagtt acaggccatg gtccaaatgt agtttcattt
     7741 tgacttgtta caaaaactgc tgttcttaat ccatacttat taaatctttc tgtacatttt
     7801 ataaagtgtt ctaaatttaa tccactatat ctatgtggat aaaagttatg acatcctatc
     7861 aaattttctt tatttggttg gtagtccatt attgtatcta tataattagt attattactc
     7921 atattgattt ctattttaag attttgagga ttaaaagtca ttaatgcctc ttcagaacct
     7981 gtaaatccca tatcaagcct tattccgtct gccttcgtct ttttaaaaaa attcaaatct
     8041 ttgtagctta tttctaattc accaaatact ctgggattta catcaagtat tacctcaaaa
     8101 cctttaaact tagcgtattc atttatactt ttaaattttt taattatatt ttctttagta
     8161 tctttaactg ataataaaca tgaaaatata cgtgtaaatc cattttcagc agctttatct
     8221 atataatcat atatttcctt agatgttgtt ttttctggat aaattgatat tcctaatctt
     8281 cccatctgtg tcatctcctt atatcctata tgtgtttaaa tttatttcga attacacgaa
     8341 acttttttaa aaatttgatg aaaaaaagtt tcatatatat ttataaaaaa agaaaagttt
     8401 accttttctt tttttataaa caatcaacaa aatatcttcg ttacttattt cataactgtt
     8461 atactttgaa atttatacta atctaatata gtttttaatt tccaatatag tttctaatta
     8521 ataagcttct tgctcttcaa ctttttcttc atgaagcaac ctcttatcgt acatcttgac
     8581 aaatggatag taaattatta ttgatatagc tatacataat aagttaagaa ctgaagctct
     8641 ccaatctcca ccagtggcta aatatgcccc tactggtcct ggtaatgtcc aaggagcagt
     8701 aactactact ctggatacta aattagcact catagcaaac catgaaactg ttgccattac
     8761 tattggtgcc acaacaaatg gtattattaa tattgggttt aatactattg gtgctccaaa
     8821 tattattggt tcatttatat tgaatattgc tggtattaca gttgcttttc ctaaagactt
     8881 tccataagct gattttgctc taaatgcaag aagtaaagct aagcctattg tagctccaga
     8941 acctccaatc catataaacc attgaaaaaa gggttcagca gcaatacctg gaactgcttg
     9001 acccgcagca tatgaagctg tattagcatc taataattgt atccataaag gtcttgccaa
     9061 tgaacctatt attgatacac catgtattcc aaatgaccaa aaaaacgtta ttaaaaatat
     9121 tagtataatt actgatggta atgtgtcagt ggctgaaact aatggagtta ctaatttagc
     9181 tatgaagcca tgccaatcaa accctaggaa ataagtaaca atagccatta aaattattat
     9241 taatgcggct ggtgtcagag cttcaaaaga ccttgtgact gatgctggaa ctgcttctgg
     9301 catggttatc ttgaatttag atttgtcagt aaatctataa atctcaacac ctattataga
     9361 tgttacaata gcaacaaaca ttgaacctcc accaagatta gccattggca tttgccatcc
     9421 ttgaggaatt gaattatacc aagctaaaag ttctggactc ttttcaacta tctttaataa
     9481 atcaggggta agaccctcta ttgcagtagg aactattgtc aaaaggaatg tagctgtagc
     9541 taataaacct cctgtcaatc catctaactt atatgacttt gccaaactat aacctatgcc
     9601 aaatgtagaa tatactgata taataaaaaa tgtcattcta tatggtagta atattgtaac
     9661 tgcattttcc tttagatact gagttacccc ccatgaatct ggtaatggag gaaatgctaa
     9721 tatcaagaaa aatgacccaa ctattataag tggtaatgaa gctacgattc catctcttac
     9781 agctcttaag tgacgttgtt ctgccaatct ggccatagaa gttgataatt tagtgtctaa
     9841 aaattctgta aatttatcta gcatcttttc tctccttaat taatatttat ttttattatc
     9901 attaaaattt ttataaaatc aactttcttc taatctaaca acactgcaac tactatttac
     9961 tcaacagttc ttgaacttgt tttaataact ttggtcctcc taatggacta taagcttgtg
    10021 cttgtattaa tgcacatggt acactaacct catcagcaaa agcttttaaa tagtcaaatc
    10081 tatgtctgac ttgtggagct accatagcaa tatcccatcc atttttaact tcattctcaa
    10141 actcttgagt accaacagcc aaaacatcta tatctattcc attttttgct ccttcttttt
    10201 gtaatgcatt aactgctata gcacttgaca tccctgatga acaaacaaat aatactctca
    10261 tattgattcc ccctgttttt ttatttcttt tcaatcatta tagcatgtat ttttgattac
    10321 aggaatacgt ttgtatatat tttttcatat tttacatttt aaattgtaat tttatttcat
    10381 gtatttatat cttgctttaa ttgctttatt tatctaatca ttcttttgtc taaaattaca
    10441 cactacttaa aatactcata tcttttctta aaaaagaaaa aagactatct tattattaac
    10501 ataaaatagt ctcaatactt ttttaaatat gtattccttt atatatccag aagtaaccta
    10561 ttattgcaaa tgataaagca aataaaatat acagtctaaa aaatcccaaa gaaaaacctc
    10621 tcttagtttc aacaaagtat cttgtatatg caactgcaaa agatgtaatt aatgaatttc
    10681 ctataacaac tccaaatcta ttatctattc caaatataga taataacaaa ggaaaaacta
    10741 cagtacttac tagagatgaa actattaata ttaatccact tttaaaactt atagttggaa
    10801 tattataatc tgttctttga tttttctcca tttgtttctt agtattttta ttactttttg
    10861 tactttttgc atttttataa ttattatttt tctttctttt ctttttttta ttattagaca
    10921 tgtcatttac ctccaaaagg tttaggtgtt atattcccta aaataacttt atttttagcc
    10981 cctgttgcag atattacatt agaaaaagaa ttatttaatg tttcattgcc caagattaca
    11041 aatgccaaag cctcctttgc atcagaagaa tacccatatt catcttgagt taatacttca
    11101 acctctaata ataactcttt tatatatcct attaaagtta gattatgagc tcctccacct
    11161 ccaattaata aagtatctac attaaccttt ggaagtatga agtttttata attataatat
    11221 atagcttttg ccgtaaacat agttactgtt gctatcaaat catatttatc tacatgttta
    11281 tatttgttaa gcatgttatc caaataaact tgtccaaata cctctcttcc agtgctttta
    11341 ggtggcgctt gactaagata cttatgactc atcaaatctt ttaacatttc ttcattaatt
    11401 ttaccttttg atgcaaagta tccattttta tcatactttc tattaaataa tctttgacat
    11461 actccatcta ttatcatatt tcctggtcct gtatcaaatg caaatacatc atctatgtta
    11521 catgatttag gtattatagt aatatttcct attccaccaa tattttgaag tgctaaattt
    11581 ttattgttat cactatataa taaaaattca ctatatggta ctaatggagc accttctcca
    11641 cctgctgcta tatccattac tctaaaatta gatattacct ttgtatttgt ttcatatgct
    11701 ataactgctg gttcacctat ttgaagcgtt gaagatacaa aattattata acttcttggt
    11761 atatgaaaga ttgtttgacc atgagatgcg ataaaatcca aatttgctat atgaaagttt
    11821 gctttatgac acactgattt tacagcttta ctaaataaat atccaagctt aaaatttaag
    11881 ctacagatta aatcaacact cgattcctct tctatacagc actttcttat ttcgtctttt
    11941 atatctttag gaatttctag agttataaat tctatttgct taactttagt atttctacca
    12001 ttcccactaa tttcagctag tactgcatca actccatcta aggaagttcc tgacattaaa
    12061 cctacactat acatagttat catcctttta agtttatagt tattcctccc caaatcaagg
    12121 tattaagttt aataatttta gaattaatct tatttcatct ttaattacga taatttactc
    12181 ttatgaaatt aattgagtta atcttataca cgttttttac cttagctaaa tcttacatta
    12241 atttattttc agtgtcaatg taatactatg taattttttt tcacaaacaa aacatattct
    12301 ctctccaaaa accatattaa aatagttact acaaataaaa aacataccag gtacatatct
    12361 gatatgtttt tcatatataa taattattat tttataatat agatagaaca tcatttaagg
    12421 cactaattaa atcttcacta ttttcaagaa ctaaatttat tcttattgta tatgtcgatt
    12481 tgtatatgat gatagatttt atagtctaat aacctaacta tctaggctta aataatctga
    12541 tatatcataa aaatagatat taaaaattgt atatgaacca ttttccgaac atacctcaat
    12601 ctcatgccct aaactatcta tcattttctt tacaaaatac atccccattc cagtagactt
    12661 atacaattga tttcttccat ttttccccgt aaatcccttg tcaaaaaccc tagatatatc
    12721 ttcctcattt atacctatac caaaatcttt tatagataat accactccat tttctaactt
    12781 ttttgaatta aattcaatct taccaacttc ctttgaatac tttatagcat tattaataat
    12841 ttggtctaaa acatatgaca tgcatttaga atctgtgtaa acatcatttt ctaattcatt
    12901 caaactaact tctacattat tttttataag caaaaatgaa ttatttttta tagacatttt
    12961 taccaatttt tctaaattaa atttatttat aaatacatct tcaaacatag tgttgcttct
    13021 actactatac aaaatagagt ttactaaaaa atttatcttt gcaacctgat tcttaatatc
    13081 caaactatct tctatgctac ttaacctatc tgtaattata cttaaagaag atattggaat
    13141 ttttatttca tgaacccatt tagctatgta ctcatccaat tcttttaatg attgtttata
    13201 agtctctatg tcactattat tttttatttt taagttttct ataatatctt ttattagatt
    13261 ttcttcaaaa gattgtccat caactaaata actgtctatt tcaccactat tttctataca
    13321 attatataaa tttgtgtaac tatttctaaa atttatataa tcta
//
LOCUS       DAELLV010000094        13256 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.094, whole genome shotgun sequence.
ACCESSION   DAELLV010000094 DAELLV010000000
VERSION     DAELLV010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13256
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.094"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(29..691)
                     /gene="zmp1"
                     /locus_tag="KPD58_002089"
     CDS             complement(29..691)
                     /gene="zmp1"
                     /locus_tag="KPD58_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease Zmp1"
                     /protein_id="HBG0265854.1"
                     /translation="MRPSKKLLIAIISIFLISSVPVSAHADSTTIQQNKDTLSQIVVF
                     PTGNYDKNEANAMVNRLANIDGKYLNALKQNNLKIKLLSGKLTDEKEYAYLKGVVPKG
                     WEGTGKTWDDVPGLGGSTVALRIGFSNKGKGHDSINLELHETAHAIDHIVLNDISKSA
                     QFKQIFAKEGRSLGNVNYLGVYPEEFFAESFAYYYLNQDTNSKLKSACPQTYSFLQNL
                     AK"
     regulatory      complement(791..876)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(1061..3979)
                     /locus_tag="KPD58_002090"
     CDS             complement(1061..3979)
                     /locus_tag="KPD58_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021362860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HBG0265855.1"
                     /translation="MKKGNRKALLISLIMILSMVVSTIYPTVSYASELGENSQIQSGS
                     TNSSTGEEKESDNKKPEQTPEKDKATDNKKPEQTPEEEKPTDNKKPEQTPEEDKSTDN
                     KKPEQTPEGEKPIDNKKPEQIQEEDKSTDNKKSEQALEDEKPLDNKNTEKTPEEDNLL
                     EDENLLKVLEEELNEENEDYGFVVKINNNTIETESMKKISFNLTYTPTSKGIQAGDSI
                     TFKVPDVFNKVNLDYTSECFDKTESNGEYTLTFRELPNGQSVMQGKIGLEAYVKKVDE
                     DTNAKIHIETTGKIESGSGDIDVEIKPGDKTDVPDAKGTLKKLVEGKKSTTVFMPVKN
                     KDINYSIQVNEKQEELKDIILYDELPEGLTLINGSVSVVTSDGKEVSDFNIEQSKNSI
                     SVNFGNIDKSYTVKYKARISDKNAKHGNKYKNVARIESDGKKIQEDDATVSIFDRGDD
                     YLLTKGHSGATNITQVGQVINYQISINDDKSPISNVVITDNIPEGMRLTTSGEAGHDF
                     RVVEIPMNGSWTPWSKEKIANNISYKVEEKRNESGQVDKVITGFTINLSKEEVESKFF
                     IAYTLKVISIEDSYINRAVLDANNSEIDKNDEINFKKNSGLISAKKEVDKKVLNSSDN
                     QIVKYKINMSTYGVYDAGQVNLLDEVNSVLEISNIKYSDNLELKKEAGDGKNTIRLVN
                     KYEFKQIKEGEPVQSWVTFDANFTNVKVGETIRNVAQINGSSPPGVETTKQGYAFEAK
                     KVDALDKNVLSGAKFNLEDAFGNIVVKDLVSDEDGIIQSSVKNPGTYYLVEIMAPRGY
                     EKLKDKVKVEIGNEDIGKIVDIGNIENLKQENPPVNPPIPPDTDEPIVNPPVPPSTDK
                     PRKPSSSSDTENTIVINPPVPPSEDIINPPIPEVLNPPVPPSEEMIETPVKQIIPIPE
                     VVKPSVSEEKNNKVKDDTLVNPPVPPKTGDSTTIIGEILLVIGAIVGLIVLRRNKNTN
                     "
     regulatory      complement(4097..4182)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(4309..5487)
                     /locus_tag="KPD58_002091"
     CDS             complement(4309..5487)
                     /locus_tag="KPD58_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBG0265856.1"
                     /translation="MFSKILVKTFIRDSENVQNTDVRNKYGYVAGVVGILSNLLLFVI
                     KVFIGMLTSSIAIMADAFNNLSDMASSAITMIGFKLASKPADKEHPFGHGRIEYLSAL
                     IVAFMVMLVGLQFVKSSIERIVNPIPVKFEVIPLILLIASIMIKIWLSRFNKFMGNKI
                     DSSALKAVSLDALGDVFTSSCVVISFIVARFTNFPIDGYVGIVVSLVILYAGFSLVKD
                     TINPLLGEAPDEEMVNSIIELLLSYKYIIGTHDLIIHNYGVGRCIASIHAEIPSNIDI
                     MEIHEIIDTAEREISEKLDIYLVIHMDPICLEDKEVMSAKKELEEILKKNSLVKSMHD
                     FRIVGKGSKKNLIFDIVVNPSEFSKNMSEDDLKEDITKLVKEINPEYNCVIVVDKDFI
                     "
     gene            5862..6011
                     /locus_tag="KPD58_002092"
     CDS             5862..6011
                     /locus_tag="KPD58_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBG0265857.1"
                     /translation="MFFISYIYTKSPIEFDTPLQKEAYKILQKLDIDFECVDTDEAIT
                     MEDCV"
     gene            complement(6112..8886)
                     /locus_tag="KPD58_002093"
     CDS             complement(6112..8886)
                     /locus_tag="KPD58_002093"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HBG0265858.1"
                     /translation="MRYYNKPTKEVLKYLKTNPEIGLDDNEVEERKLRYGLNEFTIKE
                     GRTFWDELGESLTEPMILILIGAAVISSFVGELHDALGILGAIFIGISIGIITEGKSK
                     KAAHALSKLTENIEVKVLRNGKIIKISKNDLVPGDIVYIETGDMIPADGRLIQSINLK
                     LREDMLTGESDDVAKNADAVLDMEVVYSKTEIIEQDAIPAKQVNMVFGGTLVAYGRGI
                     MVVTHTGDKTEMGKIAQNLSNEDQQTPLQIKLGKLGAKIAGISGVIATLLCMFMIIQM
                     QRKGMLILDTSSVLSFLQSLEPAKNAYMVCIALIVATVPEGLPTMINITLAITMQKMA
                     KINALVTKKEACETIGSVSVICSDKTGTLTQNKMMVEVAYVDGKYISGGEYQSNSYFE
                     QNCIVNSTADIEKEDNSFKYIGSATECALLLYHNDKNYNEMRKQTYLISQIPFSSEEK
                     KMSTLIRQEDSDILLSKGAPEVLLKKCSYVQQGKNIVPITPKVEKSILDEIKKLQIKS
                     MRTLGFAYKKMSNSKTEVAVTSEGELNLIGNSRSYMKEDNLVFSGFVGIVDPLREGVK
                     DSIDKAFNAGVDVKMLTGDNINTATAIGNELGLLNDGKKAVEATYIDVLTDKELREEI
                     KGISIVARSKPDTKMRIVSALQKSGEVVAVTGDGINDAPALSQADVGIAMGISGTEVS
                     KNAADIILTDDSFSTIVEGIKWGRGIYENFQRFIQFQLTVNIVAFIIAIISQLTGKDM
                     PFTTIQLLWVNIIMDGPPALALGLEPVRDYVLKRKPINRHSGIIARSMFVNIIINAIL
                     IITIVFTQSAFNILGATSEEQGTVIFSLFAFSALFNALNCREFGLNSTIPNFFKNKLA
                     LQIIVVTGIIQIIFTQVFQSFFNSVSLDFDMWIKIILFASTILLSNEFVKLILRTMKN
                     SRTMNFNSKN"
     gene            complement(9058..10314)
                     /locus_tag="KPD58_002094"
     CDS             complement(9058..10314)
                     /locus_tag="KPD58_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBG0265859.1"
                     /translation="MLKTTKISDPELYKIVADELVRQEHNIEMIASESTAPTEVLELS
                     GCVFTNKTEEGYPGARFQAGSEEADKLETLAIKRAKEVFGAEHVNVQPYSGSTANYCV
                     YSSILKPNDTVLSMRLDQGGHLTHGSAVNFLHDIYKYEFYGVDPNTGRIDYDALEAKA
                     KECRPKLIIAGASSYPRLIDYERISKVAKEVGAYFMVDMAHVAGLVAAKVIPSPVPYA
                     DFVSSSTTKTFCGPRSGIVLCKAEHAKKLDKGVFPGTLGSIHLNTVAAKAFSLLYLST
                     DKFKKIMEQVVVNAQTLASELISHGFSIVSGGTDNHIVMVDLRSKNLTGKQFEKALEY
                     VGITVNKNVIPDDPQSPFVTSGVRIGLTSISQRGLKEKEVIQIAGIMNKVAENIDNKE
                     VLDECKAEAQELISKFPLYPEGYFED"
     gene            complement(10386..11888)
                     /locus_tag="KPD58_002095"
     CDS             complement(10386..11888)
                     /locus_tag="KPD58_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBG0265860.1"
                     /translation="MRALNVVERAGNKLPDPVTIFLLLCIIVVILSAVISNLGVEEIH
                     PSTKEVVKVVNLLEKEQIQSYLGSIVTNFQSFAPLGLVLVTMLGAGVAEKSGFMEVLM
                     KKGISKVPQKLVTVAIVFAGMLSHTAADVGFIILPPLAALVFLGIGRHPLVGMFAAFA
                     GVAGGFAANVMLSTTDVLLAGFTIPAAQMMDPSYQGNPAMNLYFAFISAIVLTFAGAF
                     VTEKFIAPRFTKYNDSTNDKDIQELSELENKGIKYALLSLLVVVVVIVALCIGDNAFM
                     KDPETGSILSSNAPLMKGIVPIITIIFLTPGLVYGKVSKKIKSDKDLVSMMGSSMSDM
                     GGYIVLAFIASQFINLFNLSNLGTILSITGAKLLAESGIPSYGLIIGFILLSGFINLF
                     VGSASAKWAILAPIFVPMFMLLDFNPALTQIAYRIGDASTNPISPLFPYFPVILAFAR
                     RYDKDIGIGTVISNMIPYSVVFTLIEIIILLLFMGIGIPLGPGGGISYVL"
     gene            complement(11979..13169)
                     /locus_tag="KPD58_002096"
     CDS             complement(11979..13169)
                     /locus_tag="KPD58_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0265861.1"
                     /translation="MHELILISEKYKEDILDFFTDIHMHPELSFKEFRTTKAIKDLLV
                     SLDIEILDLGMETGVVGLLKGKYDGPTVALRGDIDALPIYEEVDIKYKSRYDGIMHAC
                     GHDIHTSCLVGCAYVLSHIRDSLHGNVKFIFQPAEEVNKGAKMLVERGVMENPKVDAI
                     FGLHNHPDIPCGKIGVKLGGLMAAVDTIKIEVNGFGGHGGIPNRTIDPIVASSAIIMG
                     IQTIVSRNISPLESAVISIGTINGGTANNVISEKVDMTGTCRSFSNEVRKKISENLEN
                     IVCEIARGYQATAKLDYLFDLPAVINSKEMYAIACKSVCDLYSEDAIVDPIPSTGGED
                     FSIFMEKAPGFFYWLGVGNKEQDCIYQWHNPKFKADKNSILVGTNVLCQSVINYMDKL
                     KNKI"
BASE COUNT         4045 a         2422 c         1559 g         5230 t
ORIGIN      
        1 ttatatttat tttaaattta ttaaaactct atttagctaa attttgcaaa aagctataag
       61 tttgtggaca agctgatttt aacttactat ttgtatcttg atttaaatag taatatgcaa
      121 atgattctgc aaaaaattct tctggatata cacctaagta gtttacatta cctaaagaac
      181 gaccttcttt agcaaatatt tgtttgaatt gtgctgattt agatatatca tttaatacta
      241 tatggtctat tgcatgtgct gtttcatgta attctaaatt tattgaatca tgccctttac
      301 ctttgttact aaatcctatt ctaagagcta ctgtacttcc acctaaacct ggaacatcat
      361 cccaagtttt tccagtacct tcccatccct taggaactac accttttaaa taagcatatt
      421 ctttttcatc tgttaacttt ccacttaata attttatttt taagttattt tgttttaatg
      481 catttaaata ctttccatct atattagcta atctatttac catagcatta gcttcgtttt
      541 tatcgtaatt tccagttgga aaaactacta tttgactaag tgtgtcttta ttttgttgta
      601 tagtagtact atctgcatgt gcacttactg gaactgaact tattaagaat attgatatta
      661 tagctattaa taatttttta cttggtctca tattattccc tcctttgagt taggccggga
      721 gtaataaatt tcaaaataaa aaagataccc aagagtatct ttgtatatac aaaaagaaat
      781 ttttactctt ttcggcaact ggctggcatg gtttactaaa ctttagcccc tatagctttg
      841 cgtgactagg tttccctagt tttgcctcat ttaattttat atttatatta tagcttatat
      901 ctttttaatt gtctatatcc ctttggaact tttttctaaa aagttattat ttttttacat
      961 aaatcttcaa aaaaaaatat cttctaacaa tattttcatt attaaatatt gttttagaag
     1021 atatttttta atataactta tatttttata atgttagggt ctaatttgta tttttatttc
     1081 ttcttaatac gataagtcct acaatagctc ctataaccaa aagtatttct ccaataattg
     1141 tagtactatc ccctgttttt ggtggtactg gtggatttac caaagtatca tctttaactt
     1201 tattattttt ttcttctgac actgatggct ttactacttc tggtattgga ataatctgtt
     1261 taacaggagt ttctatcatc tcttctgatg gtggtactgg cggattcaat acctctggta
     1321 ttggcggatt tattatatct tctgatggtg gtactggtgg atttataact atagtattct
     1381 ctgtatcaga tgaacttgat ggtttccttg gtttatctgt gcttggtggt actggtggat
     1441 ttactattgg ttcatctgta tctggaggta ttggtggatt tactggtgga ttttcttgtt
     1501 ttagattttc tatattccca atatctacta tcttaccaat atcttcatta ccaatttcaa
     1561 ccttaacttt atccttcagt ttttcatatc cacgtggagc cattatctcc actaaataat
     1621 atgttcctgg gttttttaca ctagattgaa ttataccatc ttcatcacta actaaatcct
     1681 taactactat atttccaaat gcatcttcta aattgaactt agcacctgaa agtacatttt
     1741 tatctaaagc atccactttt ttagcttcaa aagcataacc ttgcttagta gtctctactc
     1801 ctggaggact tgaaccatta atctgagcca catttcgtat agtctcccca acttttacat
     1861 ttgtgaagtt tgcatcaaat gtaacccaac tttgcacagg ttccccttct tttatctgtt
     1921 taaattcgta tttatttact aatcttattg tattttttcc atctccagct tcttttttaa
     1981 gttcaagatt atcagaatat tttatattag atatttctag aactgagttt acttcatcta
     2041 aaagatttac ttgtcctgca tcataaactc cataagttga catattgatt ttgtatttaa
     2101 caatctggtt atcactactg tttaaaactt ttttatcaac ttcttttttt gcgcttatta
     2161 atccagaatt tttcttaaag tttatctcat catttttatc aatttcacta ttatttgcat
     2221 caagaacagc tctatttata tagctatctt caatactaat tacctttaat gtataagcta
     2281 taaaaaactt tgattccact tcttctttac tgagattaat tgtaaatcca gttataactt
     2341 tatctacttg cccactttca tttctttttt cttctacttt atatgaaata ttattggcaa
     2401 ttttttcttt actccaagga gtccagctcc cattcattgg aatttctaca actctaaaat
     2461 catgcccagc ttctcctgaa gtagttaatc tcatgccttc tggtatatta tctgttataa
     2521 caacattact tattggtgat ttatcatcat ttattgaaat ctgatagttt attacttgac
     2581 caacttgagt aatatttgtt gctcctgaat gtcctttagt aagaagataa tcatctcctc
     2641 tatcaaatat acttacagta gcatcatctt cttgtatctt cttaccatca gattcaatcc
     2701 tagctacatt cttatattta ttaccatgtt ttgcattttt gtcactgatt ctagccttat
     2761 acttaacagt atacgattta tctatatttc caaaattaac tgaaatagaa tttttactct
     2821 gttcaatatt aaaatcacta acttcttttc catcacttgt tactacactt acagaaccat
     2881 ttatcaatgt caatccttca ggaagctcat catacaaaat aatgtctttt aactcttcct
     2941 gcttttcatt tacttgtata ctgtaattta tatctttatt tttaactggc ataaatacag
     3001 tagttgattt ttttccttca acaagctttt taagtgttcc tttagcatct ggaacatccg
     3061 ttttatctcc aggttttatt tctacatcta tatctccact tccactttct atttttccag
     3121 tagtttcaat atgtattttt gcattagtat cttcatcaac ctttttaaca tatgcttcca
     3181 aacctatttt accttgcatt acactttggc cattaggtaa ttctctaaat gtaagtgtat
     3241 actctccatt actttctgtt ttatcaaagc attcacttgt ataatctaaa ttaactttat
     3301 tgaatacatc tggcacctta aaagttattg aatctcctgc ttgtattcct tttgatgttg
     3361 gagtataagt taaattaaat gatatttttt tcattgactc cgtttctatt gtattgttat
     3421 taatttttac aacaaatcca tagtcttcat tttcttcatt aagttcttct tctaaaactt
     3481 tcaataaatt ttcatcttct aatagattat cttcctctgg agttttttct gtatttttat
     3541 tgtctagtgg tttttcatct tctaaagctt gttctgactt tttattatct gttgacttat
     3601 cttcttcttg tatctgctct ggctttttat tatctattgg cttttctcct tctggagttt
     3661 gctctggctt tttattatct gttgacttat cttcttctgg ggtctgttct ggttttttat
     3721 tatctgttgg tttttcttcc tctggggtct gttctggttt tttattatct gttgccttat
     3781 ctttctctgg agtctgttct ggctttttat tgtcgctttc cttctcctct ccagttgatg
     3841 aattagttga accactttga atctgactat tctctcctaa ttctgaagca taagatacag
     3901 ttggatatat tgtagacacc accatactta aaatcataat taaactaatt aataatgcct
     3961 ttctatttcc tttcttcatg taaatctcac ccctttaggt tacctataat ttataatcta
     4021 atcttccatt aatattgtat ataaagtaat ttttactaaa aaattataca aaaaaagcta
     4081 tgaaaataaa taaatttata ttcatagccg gttgcaccac tgactcagta tatccataag
     4141 gtgcccaata atagaatata cttcaacatc tctataatat ttcataaaaa caattaaaat
     4201 tgctttattt atattaaaaa tacaattata tacttctatt atattatgcc tctatactat
     4261 tgtctatcct ttttacattt tttaccttat gatgagatta attaataatt aaataaaatc
     4321 cttatctaca acaattacac aattatattc aggatttatc tccttaacta attttgttat
     4381 atcttctttt aagtcatctt ctgacatgtt tttggaaaat tcggaaggat taactactat
     4441 atcaaatatt aaattcttct tgcttccttt tccaactatt ctaaaatcat gcatagattt
     4501 tacaagacta tttttcttaa gtatttcttc aagttctttt ttggctgaca taacttcttt
     4561 atcttcaaga catattgggt ccatgtgaat tactagatat atatctaatt tttcagatat
     4621 ttctctctct gcagtatcta taatttcatg tatttccatt atatctatgt tggatggaat
     4681 ttctgcatgt atagatgcaa tacatctacc aactccatag ttatgaataa ttaaatcatg
     4741 tgtacctatt atatatttat aagataacaa tagctctata atagaattta ccatttcttc
     4801 atccggtgct tcccctaaaa gaggatttat agtatctttt acaagagaga aaccagcata
     4861 aagtatcact aatgatacta cgataccaac atatccatct atgggaaaat tagtaaatct
     4921 agctacaata aatgatataa ctacacaact tgaagtaaat acatctccta aagcatcaag
     4981 tgaaactgct tttaaggctg aagaatcaat tttatttccc ataaatttat taaaacgact
     5041 caaccagatt ttaatcatta tagatgcaat taaaagtata agtggtatta cttcaaattt
     5101 tacaggaatt ggatttacta tcctttctat agatgacttt acaaattgaa gtccaaccaa
     5161 catcaccata aatgctacta ttaatgctga taaatactct attcttccat gaccaaaagg
     5221 gtgttcttta tcagctggct tacttgctaa tttaaaacct atcatagtta ttgctgaaga
     5281 tgccatatca gaaagattat taaatgcatc agccattata gctatactag atgtgagcat
     5341 tcctatgaat acctttatca caaataaaag taaattggat aatataccta ctacaccagc
     5401 tacataacca tacttatttc taacatctgt attttgaacg ttttcgctat ctctgataaa
     5461 tgttttgact aagattttgg aaaacatgaa acttacctcc ctgcccttta ttttattaac
     5521 aaattttata atctatatct ttatttatga taacataatt tatatataaa attcttaaaa
     5581 atcactctac ttcagtaaaa tattattcta tatatctatt tagatacgaa ttttatattt
     5641 agtaaagttt ttatggctta gtgtctgttt aatagattct atataagttt ttacaaagat
     5701 tatttattat gataatatca tttttcccaa gtattactta gaatatgatt tttttgtttt
     5761 gtctgtacat attttaaatt tatactatat ttatctatat tttataacct tatgatacac
     5821 tataactata agtttttaaa tggaaaggaa tgaaataaat catgtttttt atcagttata
     5881 tttacacaaa atcacctatt gaatttgata ctcctttgca aaaagaggca tacaaaatat
     5941 tacaaaaatt agatattgac tttgaatgtg ttgacacaga tgaagcaatt acaatggaag
     6001 attgtgttta gattaataaa aaattaaata tgaaaatggt aatataaaac aacattaaac
     6061 aaacaaaaac cgcaaagtta caatatatag tactttgcgg tttcatagga attaattttt
     6121 actattaaag ttcatagttc tactattttt cattgttcta agaattagct ttacaaattc
     6181 atttgacaat aggatagtgg atgcaaatag tattatcttt atccacatat caaagtccaa
     6241 agaaacagaa ttaaagaaac tctggaacac ttgtgtaaat attatttgaa ttatccctgt
     6301 aactactatt atttgcaatg ccagtttatt tttaaagaaa tttggtattg tactatttaa
     6361 accaaactct ctacaattta atgcattaaa aagggcactg aatgcaaata aagaaaatat
     6421 aacagtacct tgttcttctg atgtagctcc caatatatta aaagctgatt gagtaaatac
     6481 aattgtaatt ataagaatag catttataat aatatttaca aacatagacc tagctattat
     6541 tccagaatgt ctatttattg gttttctttt caatacataa tctctaacag gttctaatcc
     6601 taatgctagg gctggaggac catccattat tatatttacc cataataatt gtattgtagt
     6661 aaatggcata tccttaccag taagttgaga tattatagca attataaatg ctactatatt
     6721 tacagtaagc tgaaactgta taaatctttg aaagttctca tatatacctc ttccccattt
     6781 tattccctct actatagtac taaagctatc atcagtcaat attatatctg ctgcattttt
     6841 agatacttct gttcctgaaa tacccattgc aattccaaca tctgcttgac taagtgctgg
     6901 ggcatcattt ataccatcac ctgttacagc tacaacttcg ccactttttt gaagcgctga
     6961 aactattctc atttttgtat ctggtttact tcttgcaaca atagatattc ctttgatttc
     7021 ttctctcaat tctttatctg tcaatacatc tatatatgtt gcttcaactg cctttttccc
     7081 atcatttaat aatcctaatt catttccaat agctgttgct gtatttatat tatctcctgt
     7141 aagcattttt acatctacgc ctgcattaaa tgctttatca atggaatctt tgaccccttc
     7201 tctcaatggg tccactattc ctacaaatcc actaaaaact aaattatctt ccttcatata
     7261 acttcttgaa tttcctatta aattcaattc accttctgaa gtcactgcaa cttctgtttt
     7321 agaattactc atttttttat aagcaaatcc taatgttctc atagatttaa tttgtaactt
     7381 cttaatctca tctaaaatac ttttttctac ttttggagtt attggaacaa tattttttcc
     7441 ttgttgtaca taagaacatt tttttaataa tacttctgga gcaccttttg aaagtaatat
     7501 atctgaatcc tcttgacgaa ttaatgttga catcttcttt tcttctgaac taaatggtat
     7561 ttgtgatatc aaatacgttt gttttctcat ttcattatag ttcttatcat tatgatataa
     7621 aagtaatgca cactctgttg cgcttcctat atatttaaat gaattatctt ctttttctat
     7681 atctgcagtt gaatttacta tacagttttg ttcaaaatag ctattacttt gatactctcc
     7741 tccacttata tatttaccat caacatatgc tacctctacc atcattttgt tttgtgttaa
     7801 agtccctgtt ttgtcggaac atattacaga aacagaacct atagtttcac aggcttcttt
     7861 ctttgttact aatgcattta tctttgccat cttttgcatt gttatagcta aggtaatatt
     7921 tatcatagtt ggcaaacctt ctggaacagt tgcaactatt aatgcaatac aaaccatata
     7981 tgcattttta gcaggttcta atgattgtaa gaaagataat acactgcttg tatctaaaat
     8041 taacatacct tttctctgca tctgtataat cataaacata cataataaag tagctattac
     8101 gcctgatatt cctgctattt tagcacccaa cttccctaat ttaatttgaa gtggagtttg
     8161 ctggtcttca tttgataaat tctgtgctat cttacccatc tctgtcttat caccagtatg
     8221 agtgacaacc attatgcccc taccatatgc tacaagagtc cctccaaata ccatattgac
     8281 ttgctttgca ggtattgcat cctgttcgat tatttctgtt ttagaataaa ctacttccat
     8341 atctaataca gcatcagcat tcttagctac atcatcagat tctcctgtta gcatatcttc
     8401 tctaagtttc aaatttatag actgtataag tcttccatct gctggtatca tatccccagt
     8461 ttctatatat acaatatctc ctggtactaa atcatttttt gatattttaa ttatcttccc
     8521 atttctaagc acctttactt ctatattttc tgttaatttt gacagggcat gagctgcttt
     8581 tttagatttt ccttcagtta ttatgcctat agaaattcca ataaaaatag cccctaaaat
     8641 acctaatgcg tcatgtaatt ctcccacaaa agaacttatt actgctgctc ctatgagaat
     8701 taatatcata ggttctgtca aactttctcc caactcgtcc caaaatgttc taccttcttt
     8761 tatagtaaat tcatttaatc catatctaag cttcctttct tctacctcat tatcatcaag
     8821 tccaatttct ggatttgttt ttaagtattt taatacttct ttggtaggtt tattataata
     8881 cctcataact ttatccccct tgttaattat ctataaggaa ttatatgctt atgatagctt
     8941 gtatattact tttcaaaaaa aataaggtgt tgattaatca acaccttata gtggttatat
     9001 attattaaat tttatgttac aggctttata aattgtgagc tatacaaatc aaaatattta
     9061 atcttcaaaa tacccttctg gatataatgg aaacttagat ataagttctt gtgcctctgc
     9121 tttacattca tctaatactt ctttattatc aatattttct gctaccttat tcattatccc
     9181 tgctatctgt ataacttctt tttctttcag acctctttga gatattgatg taagacctat
     9241 acgaacacca ctagttacaa atggtgattg tgggtcgtca ggtataacat ttttattaac
     9301 tgttattcct acatattcaa gagctttttc aaattgtttt cctgtaagat tcttacttct
     9361 taagtcaacc attactatat gattatcagt acctccactt acaatactaa aaccatgtga
     9421 aatcagttca gaagctagag tctgagcatt tactacaact tgctccatta tctttttaaa
     9481 tttatctgta cttaaatata atagtgaaaa ggcttttgct gcaactgtat taagatgtat
     9541 agaccctaat gttcctggaa aaacaccttt atcgagtttc tttgcatgtt ctgctttaca
     9601 aagtacaata ccacttcttg gtccacagaa agtctttgtc gtagatgaag aaacaaaatc
     9661 tgcataaggt acaggactag gtattacttt cgctgcgact aatcctgcaa catgtgccat
     9721 atctaccata aagtaagctc ctacttcttt tgcaaccttt gatattcttt cataatctat
     9781 taatcttgga tatgaacttg cccctgcaat tataagtttt ggtctacatt cttttgcctt
     9841 tgcttcaaga gcatcataat ctatacgtcc agtatttggg tctaccccat aaaattcata
     9901 tttataaatg tcatgtaaaa aattaactgc gctaccatgt gtcaagtgtc caccttggtc
     9961 aagacgcata cttaaaacag tgtcatttgg ttttaatatt gatgaatata cacaatagtt
    10021 agctgtagaa cctgaatacg gttgtacgtt tacatgttct gctccaaata cttcttttgc
    10081 acgttttatt gctagggttt caagtttatc tgcttcttca gaacctgctt gaaaacgagc
    10141 ccctggatag ccttcttctg ttttatttgt aaatacacat ccacttaatt caagtacttc
    10201 tgttggtgct gtactttcag aagctatcat ctcaatattg tgttcttgtc taaccaactc
    10261 atcagctaca atcttgtata actctgggtc tgaaattttt gttgttttta acataataat
    10321 cctcctctta attaaagaat atattttatt agtttaatct tcattgataa atttattata
    10381 atggtctata aaacgtaaga tattcctcca ccaggtccta atggtatgcc aatacccatg
    10441 aatagtaaca atattattat ttctattaat gtaaatacca ctgagtatgg aatcatattc
    10501 gatatgacag tacctatacc tatatcttta tcatatcttc tggcaaaggc caatataact
    10561 ggaaaatacg gaaaaagtgg acttattgga tttgttgaag catctccaat acgataagct
    10621 atttgagtta atgctggatt aaagtctagt agcataaaca tgggaacaaa tataggtgct
    10681 aaaatcgccc attttgcaga agcacttcct acaaataaat tgataaatcc agatagcaat
    10741 ataaatccta ttatcaatcc atagctaggt attcccgact ctgctagtaa ttttgctcct
    10801 gttattgata aaattgttcc taagttacta agattaaata aattaataaa ctgggatgct
    10861 ataaatgcta ggactatata tccacccata tcactcatac tactacccat catagatact
    10921 aaatctttgt cactttttat cttcttactt acttttccat acactaatcc tggagttaaa
    10981 aatataatag tgattatagg aactatacct ttcattaaag gtgcattact agatagtata
    11041 gaaccagtct ctggatcttt cataaatgca ttgtcaccta tacataaggc aactatcact
    11101 actactacaa ctaacaatga taataaagcg tattttatac ccttattttc aagttcactc
    11161 aattcctgta tatccttatc atttgttgaa tcattatatt ttgtaaaacg aggagctata
    11221 aatttttcag ttacaaatgc tcctgcaaaa gttaaaacta tcgctgatat aaaagcaaaa
    11281 taaagattca ttgctggatt gccttggtaa gatgggtcca tcatttgagc tgctggtatt
    11341 gtaaatccag ctaacaatac atctgtagta ctaagcataa catttgctgc aaatccccct
    11401 gcaacacctg caaaagctgc aaacatacct acaagaggat gtcttccaat tcctaaaaac
    11461 acaagtgctg caagtggtgg taatattata aatcctacat ctgctgctgt atgtgataac
    11521 atacctgcaa atacgattgc tacagttaca agtttttgtg gaactttact tatacccttt
    11581 ttcattaaca cttccataaa tccagatttc tcagcaacac ctgctccaag cattgtaacc
    11641 agtactaatc ctaaaggagc aaatgactgg aagtttgtaa ctatgctacc taaatatgat
    11701 tgaatctgct ctttctctag tagatttact acctttacaa cttcttttgt agaaggatgt
    11761 atttcttcta ctcctaaatt agatataact gctgacaata ttacaacaat tatacatagt
    11821 aataaaaata tggttactgg gtctggaagt ttatttcctg ctctctctac aacatttagt
    11881 gccctcatca ataatccact atctttgttt ttttcctgtg aattaatttt cctcaaacta
    11941 ataccccctc ataaacttta tctccagaat gctttatctt aaattttatt ttttaattta
    12001 tccatataat ttataacaga ctgacatagt acatttgtac ctaccaatat agaattttta
    12061 tcagctttaa atttaggatt atgccactga tatatacaat cttgttcttt gttgcctacc
    12121 cctaaccagt aaaaaaatcc tggggctttt tccatgaata tagaaaaatc ttctcctcct
    12181 gtagaaggta ttggatcaac tatagcatct tctgaataca aatcacacac agacttacaa
    12241 gctattgcgt acatctcttt actatttata acagctggta aatcaaataa ataatctagc
    12301 tttgcagttg cttgatatcc acgagctatc tcacaaacta tattttctaa attttctgat
    12361 attttctttc gtacttcatt actaaagctt ctacatgtgc ctgtcatatc taccttttct
    12421 gaaattacat tatttgcagt ccctccattt atagtaccta ttgatataac agctgattct
    12481 aatggactta tgtttctgct tactatggtt tgaataccca ttataattgc acttgatgct
    12541 actatagggt ctattgttct gtttggtata cctccgtgac ctccaaatcc atttacttct
    12601 atcttaatag tgtctactgc tgccattaac ccacctaact taacacctat ttttccacaa
    12661 ggtatatctg gatgattatg aagtccaaat atagcatcaa cttttggatt ttccattact
    12721 cctcgttcca ctagcatctt cgcaccttta ttgacttctt cggctggttg aaatataaac
    12781 tttacatttc catgaagact atctctaata tgagacaata catatgcaca tcctacaagg
    12841 caagacgtgt gtatatcatg cccacatgca tgcattatac catcatatct ggatttatac
    12901 ttaatatcaa cctcttcata tattggcaat gcatcaatat ctcctcttag tgctactgtg
    12961 ggaccatcat acttcccttt taataatcca acaactcctg tttccattcc taaatctaat
    13021 atttctatgt ctaaacttac taataaatct tttatagctt ttgtagtcct aaattcttta
    13081 aaacttaatt cggggtgcat atgaatatct gtaaaaaaat ctaaaatatc ttctttatac
    13141 ttttctgaga ttaaaataag ttcatgcata ctaacacctc tccaaaattt tattttttta
    13201 ttttttataa tttaactaca aattatataa gttaataatc tttataaaga tatttt
//
LOCUS       DAELLV010000095        13216 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.095, whole genome shotgun sequence.
ACCESSION   DAELLV010000095 DAELLV010000000
VERSION     DAELLV010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13216
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.095"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            150..2237
                     /locus_tag="KPD58_002097"
     CDS             150..2237
                     /locus_tag="KPD58_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG0265862.1"
                     /translation="MVVDKEIVSILQILLNSTLITTNGLQEESNSSKRQITYRINKIN
                     DMLKVKNVPLICLRADKDIIVRKETKKAIKEILEKNYSKNTYYFSKTERLLYMYLMLF
                     INMEDISINHFINSIKVSRSTVNLDFKDLIPKLEKKNIKVKNNRINGYYIVGNEMEIR
                     RVLIKNIIETLSNEKSSRVFDIFIKEYNLDSFEEAKKIILKLIKKYEITFVEDRLIEF
                     IYIFIFLKARMYSTRIVTQENVDIPNISVMSSMKEYKFAKELLEVYGSEDKIKPHNIM
                     YISAWILGISVGNYEEDTEDKDVISKIVNKMMTKFGYLSGVYYISQEKIFKQLYSHFR
                     PAYYRLLFKLPIYNPICEKVKEEYKLVYRIVSNAMKEFCGLFGEEIQEEELAYLTMHF
                     ATLFSNKKEFDGFRKRIALIVCSNGVGSSAILHAELTNLFPDLHFIPMSESVGLENIS
                     EPVDIVFTTNYNAEGLKTDVPVIKVSPVMTTKEKYKITREVYIQLGDIFLRQPKIDEV
                     MDIIKKYANVTSESILSSELLAYFTQIENFTSKEGEGPMLSEITNETLVKIKVKAKNW
                     EEAIRQSASVLVENNKVTEGYVDAMINSAKASGPYIVITKHVALPHARPEAGSKEIAI
                     GIATLDSPIEFGNNENDPVKYVFCLSAIDNNSHLRAMSELVELLEEDEFFKVLDSAKE
                     AKEIIDFIKTHEL"
     gene            2252..2539
                     /locus_tag="KPD58_002098"
     CDS             2252..2539
                     /locus_tag="KPD58_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0265863.1"
                     /translation="MYKILIACRAGVGSSLMLKIKTQQIIKENNFPIEVEHGSLDSLN
                     GFNGDAVETLIDVAEELKNKNLKFDIIGIHNIVDRNEIKTALEKFLASKEG"
     gene            2566..3837
                     /locus_tag="KPD58_002099"
     CDS             2566..3837
                     /locus_tag="KPD58_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HBG0265864.1"
                     /translation="MEFIISLLSTPAVLLGVVACIGLILQKKSAVEVFTGSSKTLIGF
                     LIFGIGASAMTGALQNFNKLFQHGFGITGVIASPEAATALAQGTYGFAVSCTLILGFI
                     LNLVFARITKFKNIFFTTGHSLFFSCVLVLIMKAHGFDNTITILIGGTILGFMSAALP
                     QFCQPFMRELTGGDEQAIGHFNMIGYGLSGYIGRLFSKHKDDTTETIEFPKWLSIFRD
                     FLMGLSIVMLILFYIATLKAGQAFTQEIAGSTHWLVFPLIEAFTFVAGMSILMSGVRM
                     FLAEITAAFVIISEKYIPGSRPALDVPTVFPYAPNAVIIGFISAYAAGLLAIAIMASF
                     PSIFPVVIIPAAHICFFSGGTAAIFGNTSGGWRGAIAGSFVVGLLLAFLPVILYPVYG
                     TLGIEGATFPNIDYNVVGSILHNILQFIKPI"
     gene            3895..4494
                     /locus_tag="KPD58_002100"
     CDS             3895..4494
                     /locus_tag="KPD58_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG0265865.1"
                     /translation="MENTLSEFLDNISNELSEFIASIDETSINKACELILEAEKNHGR
                     VHVTGIGKPGHVSGYISSLLSSTGTSAYILHGTEAVHGSSGQVVEGDVVIAISNSGET
                     QELKATLKTLKVNGAKIIGVSGNESSFLKNISDIFLFAGVKQEGDCLNKAPRASILAE
                     TIILQSLSVVLQYAKGLNTQQYLKWHPAGSLGKSIREGI"
     gene            4499..5404
                     /gene="dapA"
                     /locus_tag="KPD58_002101"
     CDS             4499..5404
                     /gene="dapA"
                     /locus_tag="KPD58_002101"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBG0265866.1"
                     /translation="MKYKGIICAMITPFDENQNINSQATCQLIDYLIEKGIYGLFILG
                     TNGECHVLTDDEKVEFAKIVINHTNNRVPVFVGTGGNSTREVVDLSKRMEAIGASALS
                     IITPYFVTPTQQELILHYKTIANSVNLPIIMYNMPGKTGINIEPESVRELSKIKNIVG
                     IKDSSGKLDNMKAYIEATKNENFSVFSGSDSLILDSLKAGSQGAVAATTNFLTEIGIS
                     IYENFMKGDLEEAQKAQSSIEELRRILKLGTIPSVMKKTIVLNGINVGTARLPVTEPT
                     GEVLEEIKRVVENYRTILNKSSENR"
     gene            5510..6142
                     /locus_tag="KPD58_002102"
     CDS             5510..6142
                     /locus_tag="KPD58_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketohydroxyglutarate aldolase"
                     /protein_id="HBG0265867.1"
                     /translation="MKKMNITKRMSECGALAIVREENLNRACEIAEGCIKGGITVIEM
                     SYTLNNAGEIIQGLNKKYGETLCVGAGTVLDSETARHAILHGAQFIIAPNYNEGVAKI
                     CNRYQIPYAPGCTSLTEAVDALSLGATFIKAFPISDFYGPKLVKVFKTPIPDMPVLAS
                     GGINIENLHIWLENGVDVCGFGGLLTKGSVEDIAKNAKKIREIIKNVKKI"
     gene            6620..9127
                     /locus_tag="KPD58_002103"
     CDS             6620..9127
                     /locus_tag="KPD58_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG0265868.1"
                     /translation="MKEDIGLHRMCKKEQATKNEVISEDILKSMSIGVSKHFLDDSFT
                     IIWGNTSFYQLLGYTEEEFLVKFSSLKDYYEQNLYHFESMKNHFLEAYHHGEKSIEYQ
                     ICISANNGDPVWVIMTGILIESQDNEKSMVYILYSKINELKIKQEELSDMEKESTENF
                     KWMMSEYAGNVYISDMDTYELLYLNKHSCNTLQASANELIGRKCYEVIQGRTSPCPFC
                     TNSYLKKDDTYEWEFYNKKLKRTFIIKDRMLNWQGRRVRIELSYDMYSAEYKLAKKDQ
                     ERESILKTIPAGMVRLDARDYLTILWCNDIFLNMIEYTKEQFAEELNNQCGYLFSEDY
                     KRSKKLAQDLKESGDNIVFEAKIYTRSREERIWTVTLCYISGEDSWDGIPSFYSIGID
                     ITKERKQIEKLQHISEKDALTGIYNRAETERQIKKYFEKNLNVMGALFMIDTDNFKQI
                     NDTEGHMIGDIVLTEMASGMKKIMRDSDVVGRIGGDEFTIFMKNISSVKDAEKKAEEL
                     LYMFRHLFQKEKSFLKVTCSIGIAIYPKDGTTFKEIYARADKALYQAKNMGKNNYVIY
                     NQDYFKELEEFDCSSLGTVIDSEKQYAEYPDNLTRYIFRMLYQTDDIDQAINMILEVV
                     GKQFDVSRVYIFENTEDGRYTSNTYEWCNEGISSQMAYLQNSNYQDYEDYEKLFEDEL
                     VFYCRDIHTLSPKLEELFSSQGIHSTLQCAYKEDRVFSGFVGFDECTGLRLWSQEEIS
                     TLSLISQIISMFLQRKKNNKLNQQIQQYQNILDNIDQCICVINKENNFLLYVNKKFKN
                     TYSKFQIGQPYYLDSIDIHKISIIWNKKEAYLCTSMI"
     gene            9469..11631
                     /locus_tag="KPD58_002104"
     CDS             9469..11631
                     /locus_tag="KPD58_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBG0265869.1"
                     /translation="MKKIIYVLFTIELLIVSLLGLNIVKDNEINNILYNDTTSISVMF
                     KDYKRLNKNYSKWIKDIADENNVTISKYVFNNNEKLSIYTTDTSLNNNIKLKSGKFPN
                     TNSNEFISNINTNNSNQVGKFSTMSKDISILIRDFGKISEVGESGILYISTQNEDTIN
                     KILKELNVDRSIFIARLHDRYVNSNLYLNPSIIRDLILIILCFLATIIHYSISVSREA
                     SILRLNGYSKLDIIARVIKNILRIMILSSVTALLIYIIYILKLNIGVRACLYFAMFSI
                     ICILINILISILIVGFNSRSSKYMLSLNGKKSYGFVNIMHFTLKIVFISFLILSINNC
                     ILNYNMLQTQLGNLSEWDKAKNVYNLTLKDTGEQSLGKEEVIKNAKIKGFYKNLVEEK
                     DAFLIDSTNFEKLEDGSYMYEINSKGKNPEVSQYGKNIKINKNYLKVNPIYSNGKEIF
                     NLIDYDDKTINLLVPKKLQVHENEIKKEFRELFYFEKIEVENMYNKELNRELNKTKKS
                     DLNVNIIYVDNNQSYFTYNSLVMDDNRNLIDDPIAIVDIDNIDDSFYLSYISRCVYFN
                     SKKLDALADISNIIEAQGVEAHIQSLHAVYNEYGLEINKLEKYLNSEIFTIIIIAISN
                     LMITYNIVASYYERNKYKLYIKKIFGYSATARSMLLIVSLILTNIIPIGIISTRVDLS
                     NNIILFGLLILVIEVIVSIVLDKIISNSSFNKIIKGEH"
     gene            11636..12253
                     /locus_tag="KPD58_002105"
     CDS             11636..12253
                     /locus_tag="KPD58_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0265870.1"
                     /translation="MISLINISKKFKDKSIIDKFNLEISKGEFVAITGNSGSGKTTLL
                     NIMGLLEKPNSGDIIINNIKNPNSKQIRILQRDFYGYLFQNYALIENENVENNLKIAL
                     KYQKNINHNEKINYALESVGLRKYNKKKIYELSGGEQQRVALARVILKECEVIFADEP
                     TGNLDKKNRDIVFDILKSLNKNGKTIVFVTHDLELSEQADRVIKL"
     gene            12442..12786
                     /locus_tag="KPD58_002106"
     CDS             12442..12786
                     /locus_tag="KPD58_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HBG0265871.1"
                     /translation="MENLPNCPKCNSEYTYEDGTLLVCPECSHEWALGLGAEEDSNII
                     KDVNGNVLNDGDSVTVIRDLKVKGSSSSIKIGTKVKNIRLIHDSSDGHDIECKIDGFG
                     AMKLKSSVVKKA"
BASE COUNT         5180 a         1416 c         2241 g         4379 t
ORIGIN      
        1 aagtttaatt tttgtatcaa tctaaaacaa aagttatact taaatgaaat tgaaaacgat
       61 aaaattctca aataaaataa gtattgtgga ggtgatgaaa gaatccaatg ataatatgaa
      121 gactaatctt aataagaaag taggggttaa tggtagtgga caaagaaatt gtatcaattc
      181 tccagatttt attaaatagc acattgatta caactaatgg attacaagag gagtctaatt
      241 catcaaagag acaaattact tatagaataa ataaaatcaa tgacatgctt aaagtaaaaa
      301 atgtaccact tatatgttta agagctgata aagatattat cgttagaaaa gaaacaaaaa
      361 aagcaattaa agaaatttta gaaaaaaact attctaagaa tacttattat tttagtaaga
      421 cagaaagact tttatatatg tatttaatgc tttttattaa catggaggat atttcaatta
      481 atcattttat aaattctata aaagtaagta ggtctactgt aaatcttgat ttcaaggatt
      541 tgataccaaa gcttgaaaag aagaacatta aagttaagaa caatcgtata aatggatatt
      601 acatagttgg caatgagatg gaaattagga gagtgcttat taaaaatatt atagaaactt
      661 tgtctaatga gaaaagttca agggtatttg atatttttat taaagaatat aatttagata
      721 gttttgaaga ggctaaaaaa attatattaa agctaataaa aaaatatgaa attacttttg
      781 ttgaagatag attaatagaa tttatatata tatttatctt tctaaaggca aggatgtatt
      841 caactagaat agttactcag gaaaatgtag atattcctaa tatttcagtt atgagttcaa
      901 tgaaggagta taaatttgct aaggaattat tagaagtata tgggagtgaa gataaaataa
      961 agcctcacaa tataatgtat attagtgctt ggattttagg tatatcagta ggaaattatg
     1021 aagaagatac agaagataag gatgtaattt caaaaattgt aaataaaatg atgacaaagt
     1081 ttggatattt aagtggagtt tactatataa gccaagaaaa aatatttaaa caattatatt
     1141 ctcattttag acctgcctac tatagacttt tatttaaatt acctatctat aatccgattt
     1201 gtgaaaaagt aaaagaagaa tataaattag tttatagaat tgtaagtaat gcaatgaaag
     1261 agttttgcgg attatttgga gaagaaattc aggaagaaga gttagcatat cttacaatgc
     1321 actttgcaac acttttttca aataaaaaag aatttgatgg ttttagaaaa agaatagcat
     1381 taatagtttg ttcaaatggc gttggtagtt cagctatttt acatgcagaa cttacaaact
     1441 tatttcctga cttacacttt ataccaatgt cggaatcagt tggattagaa aatataagtg
     1501 aacctgtaga tattgtattt actactaatt ataacgcaga gggtttaaaa acagatgtac
     1561 cagtaattaa agtaagccct gttatgacca caaaagaaaa atataagatt acacgtgaag
     1621 tgtatattca attaggtgat atttttttaa gacaacctaa aatagatgag gttatggata
     1681 taataaaaaa atatgcaaat gtaacctctg aaagtattct ttcaagtgag ctattagctt
     1741 attttacaca gatagagaat tttacatcta aggaaggaga ggggcctatg ctaagtgaga
     1801 ttactaatga aacactagta aaaataaagg taaaagctaa gaattgggag gaagctatta
     1861 gacagtcagc ttctgtatta gttgaaaata acaaagtaac tgaagggtat gtagatgcta
     1921 tgattaactc agcaaaagca tctggaccat acatagtaat tacaaaacat gttgcacttc
     1981 cacatgctag accagaagct gggtcaaaag agattgcaat aggcattgct actttagata
     2041 gtcctattga gtttgggaat aacgaaaacg accctgtaaa atatgtattt tgtctaagtg
     2101 caatagacaa taatagccat ttacgtgcta tgtctgagct agttgaacta ttggaagaag
     2161 atgagttttt taaggtttta gatagtgcta aagaagctaa agaaattatt gattttataa
     2221 aaacacacga attataaagg aggatttaat tatgtataaa attttaatcg catgtagagc
     2281 aggagttggc tcaagtttaa tgttaaaaat aaagactcaa caaattatta aggaaaataa
     2341 ctttccaatt gaagtagaac atggttcatt agattcacta aatggattca atggagatgc
     2401 tgtagaaaca ttaattgatg ttgctgaaga attaaaaaat aaaaatttga agtttgatat
     2461 aattgggatt cacaatattg ttgaccgtaa tgaaataaag acagcactag aaaaattcct
     2521 tgcttcaaaa gagggataaa tttaagatga taaggaggaa tatttatgga gtttattatt
     2581 agtttgctga gtacacctgc tgttttgctt ggcgtagttg catgtatagg attgattctt
     2641 caaaagaaat cagctgttga agtatttaca ggctcttcaa aaacacttat cggttttttg
     2701 atatttggta ttggtgcctc tgctatgaca ggagcacttc aaaatttcaa taaactgttc
     2761 caacatggat ttggaattac tggtgttata gcttcaccag aagcagctac agcacttgca
     2821 caaggtacat atggttttgc agtttcatgt acattaattc ttggattcat attaaattta
     2881 gtatttgcac gtattacaaa gtttaagaat attttcttta caacaggtca tagtttgttc
     2941 ttttcttgtg tattagtttt aattatgaaa gcacatggat ttgataatac aatcactatt
     3001 ttaataggag gaactatctt agggtttatg tctgctgcac tacctcaatt ctgtcaacca
     3061 tttatgcgtg aattaacagg cggcgacgaa caagctattg gtcactttaa catgattggt
     3121 tatggtttat caggttatat tgggcgttta ttttcaaaac ataaagatga tacaacagag
     3181 actatagaat ttcctaagtg gttatcaata ttcagagact tcttaatggg tctatccata
     3241 gttatgttaa tcttatttta tattgcaaca ttaaaagctg gtcaagcatt tactcaagaa
     3301 attgctggtt caacacattg gttggttttt ccgcttatag aggcatttac atttgtggca
     3361 ggtatgtcaa tattgatgtc aggtgttcgt atgttcttag ctgaaataac tgcagcattt
     3421 gtaatcattt cagaaaagta tattccaggt tcacgtcctg cgctagatgt tccaaccgta
     3481 tttccatatg caccaaatgc agttattatt gggtttatat cagcatatgc tgctggtcta
     3541 ttagcaattg ctattatggc atcatttcct agtatatttc ctgtagtaat tattccagct
     3601 gctcacatat gttttttctc aggaggtaca gctgcaatat ttggcaatac ttcaggagga
     3661 tggagagggg ctattgctgg gtcatttgtt gtggggttat tacttgcatt tttgccagtt
     3721 atactatatc cagtttatgg gactcttggt atagaaggag caactttccc taatattgat
     3781 tacaacgtag ttggttcgat attacataat attttacaat ttataaaacc tatttaaact
     3841 aaattatata aaagtatagt catagttact atgatttcaa ggaggataca tattatggaa
     3901 aatacattat ctgaattttt agataatata tctaatgaat tgtcagaatt tattgcctca
     3961 atagatgaga catctataaa taaagcatgc gaattaattc ttgaagctga aaaaaatcac
     4021 ggacgtgtac atgtgactgg tattggcaaa ccaggacatg tatcgggata tatttcatca
     4081 ttgctttcat ctactggaac gtcagcatat atattgcatg gtacagaagc agtacatggg
     4141 tcaagtggtc aagtagttga aggagatgtt gttattgcaa tatcaaatag tggtgaaact
     4201 caagagttaa aagcaactct taagactcta aaagtaaatg gagcaaagat aattggtgta
     4261 agtggtaatg agagttcatt tttaaaaaat atttctgata tattcctgtt tgcaggtgtt
     4321 aaacaggaag gagattgtct caataaagct cctcgtgctt caattttagc tgaaacaata
     4381 atattacaat cattaagtgt tgtattacaa tatgctaaag gtcttaatac acagcagtat
     4441 ttgaagtggc atccagcagg aagtcttgga aagtctatta gagagggaat ataattttat
     4501 gaaatataaa gggattatat gtgcaatgat aacaccattt gatgaaaatc aaaatattaa
     4561 ttcacaagca acatgtcaat taattgatta tttgattgaa aagggaattt atggattatt
     4621 tattttgggc actaatggtg aatgtcatgt attaacagat gatgaaaaag ttgaatttgc
     4681 aaagattgtt attaatcata caaataatcg tgtacctgtt tttgttggaa cagggggtaa
     4741 ttcaactcgt gaagttgtag atttatctaa gagaatggaa gcgattggag caagtgcatt
     4801 atctattatt acaccatact ttgttacacc aacacaacag gaattaatct tacattacaa
     4861 aactattgct aattcagtta atttacctat tataatgtat aatatgccag gtaagactgg
     4921 aattaatatt gaaccagagt cagtacgtga attatcaaag ataaagaata ttgttggaat
     4981 taaggatagt agtggtaaac ttgacaacat gaaagcttat attgaagcaa cgaagaatga
     5041 gaattttagt gtgttttcag gctcagattc acttattctg gatagtctaa aagctggtag
     5101 tcaaggagct gttgcagcaa caacaaactt cctcacagag attggtatct ctatctatga
     5161 aaactttatg aaaggggatt tggaagaagc tcaaaaagct caaagtagta tagaagaact
     5221 tcgacgtata ctgaaacttg gaacaattcc atctgttatg aagaaaacta ttgtcttgaa
     5281 tggaattaat gttggaactg cacgacttcc tgtaactgaa ccaactgggg aagtacttga
     5341 agaaattaaa cgagtagttg aaaattatag gacaatactt aataaaagta gtgagaatag
     5401 ataatataaa tagggaaatt acactggtat ataattaata tatatattat aaaacatgac
     5461 taaaattaat gaataagtaa agtggtttga tgagtataga ggtgataata tgaaaaagat
     5521 gaatataacc aaaagaatgt cagaatgtgg ggctcttgca attgtaagag aagaaaatct
     5581 taaccgtgca tgtgaaattg ctgaaggatg tataaaaggt ggtataacag ttatagaaat
     5641 gagttatacc ctaaataatg ctggagaaat aattcaaggg cttaataaaa agtatggtga
     5701 aacactttgt gtgggagcag gaacagtttt ggatagtgaa acagctcgtc atgctatatt
     5761 acatggagct caatttatta ttgcaccaaa ttacaatgaa ggtgtagcga agatttgcaa
     5821 tagatatcag attccttatg ctccaggatg tacatcattg actgaggcag tagatgcact
     5881 aagtttagga gcaactttta taaaagcttt cccaatatca gatttctatg gacctaaact
     5941 agtaaaggta tttaagacac caatacctga tatgccagtt ttagcaagtg gagggattaa
     6001 tattgaaaac ctacatatat ggttagaaaa tggagttgat gtatgtggat ttggtggttt
     6061 attaactaaa ggttctgttg aagatattgc taaaaatgct aaaaagattc gtgaaatcat
     6121 taaaaatgta aaaaaaattt aacatagggc tgtatcatag tatcttataa ttactatgat
     6181 acagcctttt taatattatt caaatttaaa acagtttaca tttctattat attacatata
     6241 atttcagtaa ttttatatta atttttctat ctggaaaaaa atgatttaaa acttaagtta
     6301 tgatataatt tcattaaaag aaggcgattt ttagtaaata tgagaaaata atgtgttttt
     6361 aggagaaaag ttaaatacag atttaagtga acaagaatcg ataattaaga tattaaatgg
     6421 tgaatagggc atcaaaaaaa ttactaaatt aaaagtttta gagggagaat cttagcaagt
     6481 gtgtgtgaaa attcaatttc aaacaaaaaa taattgtaaa tgaaatcaat ttatacacgt
     6541 tgaagattca cttttatcat tatattaaaa agcagagtga attaaaagtg agtctaaaga
     6601 taggaggata attaaaacaa tgaaagaaga cattgggtta catcgaatgt gtaaaaaaga
     6661 acaagcaaca aagaatgaag ttatatcaga ggatattcta aaatctatga gtattggggt
     6721 cagtaaacat tttttagatg attcatttac aataatatgg ggaaatacat cattctatca
     6781 attattagga tatacagaag aagaattttt agttaaattt tctagtttaa aagattatta
     6841 tgaacagaat ctttatcatt ttgaatctat gaaaaatcat tttttagagg cttatcatca
     6901 tggcgaaaag agtatagaat atcaaatttg catttcagca aataatggag accctgtttg
     6961 ggtaataatg actggtatat taattgaatc acaagataat gagaaatcaa tggtctatat
     7021 attgtattca aaaattaatg agttaaaaat aaagcaagaa gaactatctg atatggaaaa
     7081 agagagtaca gagaatttta aatggatgat gtcagagtat gctgggaatg tttatattag
     7141 cgatatggat acatatgaat tactatactt aaataagcac tcatgcaata ctttacaggc
     7201 atcagcaaat gaacttattg gaagaaaatg ttatgaggta attcaaggta gaacttcgcc
     7261 atgtccgttt tgtacaaatt cgtatttaaa aaaagatgac acatatgaat gggaatttta
     7321 taataaaaaa ctgaaacgaa catttataat taaagaccgt atgttgaatt ggcaagggcg
     7381 gagagttcgt attgaattat cttatgatat gtatagtgct gaatataaat tagcaaaaaa
     7441 agatcaggaa agagaatcaa ttttgaaaac tattccagca ggaatggtta gattagatgc
     7501 aagagattat cttactatac tttggtgtaa tgatatattt ttgaatatga ttgaatatac
     7561 aaaggaacag tttgcagagg aacttaataa tcaatgtgga tatttatttt ctgaagatta
     7621 taaacgtagt aagaaactgg cacaagattt gaaggagtct ggggataata ttgtatttga
     7681 agcaaaaata tatacacgtt caagggaaga acgtatatgg actgttacat tgtgttatat
     7741 cagtggggaa gacagttggg atggaatacc atctttttat agtataggta ttgacataac
     7801 aaaggaaaga aaacagattg agaaattaca gcatatatca gagaaagatg cattaacagg
     7861 aatttataat cgtgctgaga ctgaaaggca gattaagaaa tattttgaaa aaaatttaaa
     7921 tgttatgggt gcactgttta tgattgatac agataatttt aaacagatta atgatacaga
     7981 agggcatatg attggagata tagtacttac tgagatggca tcaggtatga aaaaaataat
     8041 gcgtgacagt gatgttgttg gaagaatagg tggagatgag tttacaattt ttatgaagaa
     8101 tatttcctca gttaaagatg cagaaaagaa agcagaagaa ctattgtata tgttccgtca
     8161 tctatttcag aaagaaaaaa gttttctaaa agtaacttgt agcataggaa tagctatata
     8221 tccaaaggat ggtactacat tcaaggaaat atatgcaaga gctgataaag ccctgtatca
     8281 agcaaaaaat atgggaaaaa ataattatgt aatctacaac caagattatt ttaaagaatt
     8341 agaagaattt gattgttctt ctcttggtac agttattgat tcagaaaaac aatacgctga
     8401 atatcctgat aatcttacac gttatatatt ccgtatgctt tatcagactg atgatataga
     8461 ccaggcgatt aatatgatac ttgaggttgt tgggaaacag tttgatgtaa gtcgtgttta
     8521 tatttttgaa aatacagaag atggtagata tacttctaat acttatgaat ggtgtaatga
     8581 aggaatctct tctcaaatgg cttatttgca aaacagcaat tatcaagact atgaggatta
     8641 tgaaaagctt tttgaggatg aattagtttt ttattgtcga gatatccata cattaagtcc
     8701 taaactagaa gaattgtttt ctagtcaagg tatccactct acacttcaat gtgcatataa
     8761 agaagataga gttttcagtg gatttgttgg atttgatgaa tgcacaggac tacgtctatg
     8821 gtcacaagag gaaataagta cattatcatt gatttctcaa attatatcaa tgtttttaca
     8881 acgtaaaaaa aataataaac ttaatcaaca gattcaacaa taccagaata ttttagataa
     8941 tattgaccag tgtatttgtg tgattaataa agaaaataat tttttattat atgtaaataa
     9001 aaaatttaaa aatacatatt ctaaattcca aattggacaa ccttattatc ttgattctat
     9061 agatattcat aagatatcca ttatatggaa taaaaaagaa gcatacctat gtactagcat
     9121 gatttaatgg taaagtaaag tttatattgg acttaaatta aattttgttt taaataagat
     9181 attattttag ttgattactg aaaagactag aatctatatt aatgaattat actaattttg
     9241 taccactatt aaaatattgt ttgctaaaaa taaaaaatag ccttaaaaca gttgattata
     9301 aaaaattaac tgttttaagg ctatttttat ctttgtgacg aaatagacac ttgccattta
     9361 gaatgtgaca atatagtagt tgtttagaaa aggcacaaag cattatactt taactaaagt
     9421 aaaatgtatt ttgcttttag aataaaaaca attaggaggg agctataatt gaaaaagata
     9481 atatatgtat tatttacaat tgaactatta atagtatctt tattaggtct aaatatagtt
     9541 aaagataatg aaattaataa tattttatat aacgatacaa caagtatatc tgtaatgttt
     9601 aaagattata aaaggttaaa taaaaattat agtaagtgga ttaaagatat tgcagatgaa
     9661 aataatgtga ctatatcaaa gtatgttttt aataataatg aaaaattatc tatatatacg
     9721 actgatacaa gtttgaataa caatataaag ttaaaatcag gaaaatttcc taatactaat
     9781 tcaaatgaat ttattagtaa tataaacact aataatagta atcaagtagg taaattctca
     9841 actatgagta aagacatatc aattttaatt agggattttg gtaaaataag tgaagttgga
     9901 gaatcaggaa tcttatatat ttcaactcaa aatgaagata caataaataa aatattaaaa
     9961 gagttaaatg tggacagaag tatttttata gcaagattac atgatagata tgttaactca
    10021 aatttatact taaatccttc aataattaga gatttaatac taattatcct ttgtttcttg
    10081 gctacaataa tacattattc aattagtgtg agtagagagg cttctatttt aagattaaat
    10141 ggatactcta agctagatat aattgctaga gttataaaaa atatacttag aattatgatt
    10201 ttatcatctg tcacagcatt attaatatat ataatctata ttttaaaatt aaatataggt
    10261 gttagagcat gcttatactt tgctatgttt tcgattatat gtattttgat aaatattttg
    10321 atatctatat taatagttgg ttttaatagt agaagttcta aatatatgct atctctaaat
    10381 ggaaaaaaat cttatggttt tgtaaatata atgcatttca cattgaaaat tgtatttata
    10441 tcatttctta tattatccat aaataactgt attttaaatt ataatatgct acaaactcag
    10501 ttaggaaatc tatctgagtg ggataaagca aaaaatgttt ataatcttac tcttaaagat
    10561 acaggtgaac aatctttagg aaaggaagaa gtaattaaaa atgccaagat taaaggtttc
    10621 tataaaaatt tggtagaaga aaaagatgct ttcttaatag attcaactaa ttttgaaaaa
    10681 cttgaagatg gaagttacat gtatgaaata aattcaaagg gtaaaaaccc agaagtaagt
    10741 caatatggta aaaatataaa aataaataaa aactatctta aagttaaccc tatttactca
    10801 aatggcaaag aaatatttaa tctaatcgat tatgacgata agactataaa tttattagtt
    10861 cctaaaaagt tacaagtaca tgaaaatgag attaaaaaag agtttagaga attattttat
    10921 tttgaaaaaa tagaagtaga aaatatgtac aataaagagt taaatagaga gttaaataaa
    10981 acaaagaagt cagatttaaa tgttaatata atatatgtag ataataatca aagctatttt
    11041 acttataact cccttgttat ggatgacaat aggaatctca ttgatgaccc aatagcaata
    11101 gttgacatag ataatattga tgattctttt tatctttctt atatatctag atgtgtatat
    11161 tttaattcta agaaattaga tgcccttgca gatataagta acattattga agctcaaggt
    11221 gtagaagcgc atatccaaag tttacatgct gtttataatg agtatggttt agaaataaat
    11281 aaactagaaa aatatttgaa tagtgaaatt tttactataa taataatagc aatatcaaac
    11341 ttaatgatta cctacaatat agtagctagc tactatgaaa gaaataaata taagttatat
    11401 attaaaaaaa tatttggtta ttcagctaca gctagaagta tgttgttaat agtctcatta
    11461 atattaacaa acattattcc aataggcatt atatcaacaa gagtagattt aagtaataat
    11521 attatcttat ttggacttct aatattagtt atagaagtaa ttgtatctat tgtattagac
    11581 aagattatct caaatagttc atttaataaa attataaaag gagaacatta aaaagatgat
    11641 ttcattaata aatatatcta aaaaatttaa agataaatct ataatagaca aatttaacct
    11701 agaaatttct aagggcgagt ttgtagcgat aacaggaaat agtggttcag gtaagacaac
    11761 tttacttaat ataatgggat tattagaaaa acctaattct ggtgatatta taataaataa
    11821 tataaaaaat ccaaatagta aacagataag aattttacaa agggattttt atggctatct
    11881 atttcaaaat tatgctttaa ttgagaatga aaatgttgaa aataacttga aaattgcttt
    11941 aaagtatcaa aaaaatatca accataatga aaaaataaat tacgcacttg agtctgtagg
    12001 tcttagaaaa tacaataaaa aaaagatata cgaattgagt ggaggagaac aacaaagggt
    12061 agcattagca agagtaatat taaaagaatg tgaagtaata tttgcagatg aacctacagg
    12121 taatttagat aaaaaaaata gagacatagt atttgatata cttaaaagtc taaataagaa
    12181 tggtaaaact atcgtatttg taactcatga tttagagtta tcggagcagg cagatagagt
    12241 tataaaattg taataatatc aactataata aatataagaa agagattaca gctaaatcaa
    12301 gtttaataat aatacgaata gaatttaaac attaagtaaa ttagtagttt gtaaatataa
    12361 gtataatatg atatcataaa ggctcaaaag aaattttata taataagaaa tctaaaaata
    12421 acaaatggag gacatataaa gatggaaaat ttaccaaatt gcccgaaatg taattcagaa
    12481 tatacttatg aagatggaac tcttttagtt tgtccagaat gctctcatga gtgggcatta
    12541 ggattgggag ctgaagaaga ttcaaatatt atcaaagatg taaatggaaa tgtattaaat
    12601 gatggagatt ctgttacagt aattagagac cttaaagtaa aaggaagttc atcatctata
    12661 aagattggaa caaaagtgaa aaatatccgt ttaatccatg actcatcaga tggacatgat
    12721 atagaatgta agatagatgg ttttggagct atgaagctaa agtcttctgt tgttaaaaaa
    12781 gcgtaatatt ataaataagc tgtagtgttt attgtttttc agtacagtat aaaaccataa
    12841 gaatctatta agtaataaaa gtttgtttat ttatctttat attttcttat ggttttatta
    12901 aatataaatt ttattgaaca ccaaaatatt ctctcctcta caaataagcc aatcttattt
    12961 aaactaatat actttgtact atttatactg aaattgtaaa tttaaatgct atgttattta
    13021 ggtttgtata gttaatttat atatttctga ttttataaat tgtatttatt attatatctt
    13081 gattctaaaa tcattaaaaa tttcaacttc tataaagatt tacaatttat caaatacatt
    13141 atgagtatat aaacaattta ttattacagt gctacattta aaataagtct tagtataaag
    13201 gtattgttac aagtat
//
LOCUS       DAELLV010000096        13210 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.096, whole genome shotgun sequence.
ACCESSION   DAELLV010000096 DAELLV010000000
VERSION     DAELLV010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13210
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.096"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..591)
                     /locus_tag="KPD58_002107"
     CDS             complement(<1..591)
                     /locus_tag="KPD58_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBG0265872.1"
                     /translation="MLNETRKKQLTEVCQDLIRNASYSGQEENVVKAIEENFALLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEARIVDGKIYGRG
                     TSDMKGQVSAMMAAVSYFAEDTNKDFEGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKG"
     gene            complement(856..2070)
                     /gene="dpaL"
                     /locus_tag="KPD58_002108"
     CDS             complement(856..2070)
                     /gene="dpaL"
                     /locus_tag="KPD58_002108"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBG0265873.1"
                     /translation="MREIKWKLNNLPKSEEKEKGIEFLKDEEIAKAKAFHESFPQYEK
                     TPLVKLDNLAKSLGVSGIYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLNTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAKEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAK
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNYSSAFVSAPDWVSAKGMRILGNP
                     LRGDEKVISGESGAVTTGALVSILESEDLKDLREALKLDENSKVLLISTEGDTDPDKY
                     RDIVWNGECQSK"
     gene            complement(2684..3222)
                     /locus_tag="KPD58_002109"
                     /pseudo
     CDS             complement(2684..3222)
                     /locus_tag="KPD58_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427977.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
     gene            complement(3299..5104)
                     /locus_tag="KPD58_002110"
     CDS             complement(3299..5104)
                     /locus_tag="KPD58_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0265874.1"
                     /translation="MLSKLKEFQQEMIKYTETVASVLDVDIEIVDDRLIRISGTGLYK
                     SKINESVVTEGFIYDNVIQTGQELVVLDICDNQLCIECSHYMKCLNKVIIAVPIKYNN
                     RTIGVIGAISTDKTKKVEISAKIDNYLKFVNHICDLISMKIEEHEASKNSSRKMDMMI
                     EIIENVEKGVIILDINSKISYINNIALKKLDIDKNIIENIVNIVSVESSSNGHELLEI
                     DIDNKIYNINAKIIPVYPYINQYDKIIIFDKTYINHKGHGKVNSGWGNSDIESIIGNS
                     EAMLKVKERTKKLAKSNSTVLITGESGTGKELIARAIHAEGSRWNKPFIAINCAAIPE
                     NLLESELFGYIKGAFSGASSGGKVGKFELANEGVIFLDEIGDLSMPLQAKLLRVLQER
                     KFARIGSNKLIDLDIRVIAATNKNLLKLVNEGKFRDDLYYRLNVIPINLPPLRERKDD
                     IEAIMMKFASKYSLELGIQLNKIEENVMNMLINYNWPGNIRELENAVEYMMNLVGDDG
                     IIYKDMLPLDILNYYNINGNIYKNKDVNIIFEDDIVGGIVENQERILSIKELELTYIN
                     KLLNKYGRDTKTKKKIAKDLGIGLATLYRKLEEEQ"
     gene            complement(5500..5877)
                     /locus_tag="KPD58_002111"
     CDS             complement(5500..5877)
                     /locus_tag="KPD58_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBG0265875.1"
                     /translation="MSKVVIATEKAPGAIGPYSQAIQVGNIVSTSGQLPIDMKTGELV
                     SDPKEATKASLDNVKAILEQAGTSMDKIFKTTVFVKDLKDFNDINEVYATYFKENPPA
                     RSCVQVAQLPKDAVLEIEAMAVL"
     gene            6782..7267
                     /locus_tag="KPD58_002112"
     CDS             6782..7267
                     /locus_tag="KPD58_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265876.1"
                     /translation="MSKSELTAETTEEMLEVLSGKDYDIACHLHELGKSLDCKIEPKT
                     GARSYKIVYSTKKPKRSLFTIECNEKKWRVKANLFHLNTYKDAVEECSKTIKDSITKT
                     RTCKKCNSKCIGGSSFELDGKSYLTCIGSGHYFANMEEMDWKNLEKLITKENNIMQES
                     V"
     gene            complement(7467..8006)
                     /locus_tag="KPD58_002113"
     CDS             complement(7467..8006)
                     /locus_tag="KPD58_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG0265877.1"
                     /translation="MSKNVLIVSASPRKGGNSDLLCDQFIQGVLESKNQVEKIFLKDK
                     DINYCTGCGACYEKEANCSQTDDMGEILEKMIGADVIVLATPVYFYTMNAQMKTMIDR
                     TCSRYTEICDKDFYFIVTAADENVADMERTIEGFRGFTSCLNGSKEKGIVYGVGAWNV
                     GDIKGSKAMKQAYEMGKLA"
     gene            complement(7999..8412)
                     /locus_tag="KPD58_002114"
     CDS             complement(7999..8412)
                     /locus_tag="KPD58_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0265878.1"
                     /translation="MTISEVSKKYELSADTLRYYERIGLIPPVNRNKSGIRSFTEEDC
                     EWVNFIKCMRGAGLSIETLIEYVAMFQQGSSTIKARKELLIEQRNQLAKRIEEMQKTL
                     ERLNFKIDRYEEGIIEKEKVLKSSRNKKMEAVSYE"
     gene            complement(8448..9569)
                     /locus_tag="KPD58_002115"
     CDS             complement(8448..9569)
                     /locus_tag="KPD58_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBG0265879.1"
                     /translation="MEAKKLGFGLMRLPIKDENDVTTIDMEHLNKMVDTFLERGFTYF
                     DTAYVYHMGKSEIALRESLVKRHKRESFTIATKLPLMALKKKEEQETIFNEQLEKCGV
                     DYFDYYLLHNIGVSHYEVAKKFDSFKFIEEKKKEGKIKNIGFSFHDSAELLDKVLSEH
                     PEVDFVQLQINYLDWDNESIQSRKCYEVAEKHNKPVIVMEPVKGGTLAKIPEKAEKLL
                     NDYNPDMSISSWAIRFAASKDNVMMVLSGMSNMEQLLDNTGYMQNFKPFVQEEYNIID
                     EAVEIINESILIPCTACQYCVEGCPKNIAIPNYFALYNAEKQALNTGFSTQMVYYNNY
                     TKTYGKASDCIECKQCEESCPQHIKIIDALKNVAETFEK"
     gene            10168..11820
                     /locus_tag="KPD58_002116"
     CDS             10168..11820
                     /locus_tag="KPD58_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp21"
                     /protein_id="HBG0265880.1"
                     /translation="MNKKNLAILISGMMFFSSSSAIFADNTTKQERLIGKTRYETAVE
                     VSKLGWVQSKTAIIVNGNSIQSALCANPFAKLKNAPILLVNNNSIENSTKAELKRLGV
                     DNVYIVDSGNSISSKVENEIKSLNIKINKIVGNNIYEMSTNVLKEIDKIKKVENVAVV
                     NGLKGLSDAVSIASPAAIHNMPIILVSDENSNDSSINFLNNRSIKKSYIVGGNKILSD
                     NTVKKYPNRERISGSDRNNTNAKIIDKFYKDKKINNVFVAKDGINQENALVDSLSIGV
                     LAAKENSPIMISNGSLVNSQKEFIKNKDIDEITQVGGGYNQKAFEDILKLKNVNIRNK
                     TKKISLIQAKEILKQALPGSNILVCYYDEDDNEYDCKVIKDNILYEIEIDAYSGKIIE
                     IEKDDDLNDNNLGTQNISLDKAKSIALDAIPNGNLIYCKYDAEDNEYEAKVIKNKKTY
                     ELEISAFDGRIVDIDMDNDSDDDFDDNDKNNSNTNDNSSIISAEKAKSIMLDKVPGGK
                     IVKFKYDEDDKEYEGEIIKGKTEYEITINAITGKILEFEIDD"
     gene            complement(12179..13105)
                     /locus_tag="KPD58_002117"
     CDS             complement(12179..13105)
                     /locus_tag="KPD58_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HBG0265881.1"
                     /translation="MSNSFYQLNSAVLYFNEKRDVNRIRFNHYVMRKENIQRLKGTLP
                     RGQFMMTVRKAASDLDVSISTISRLVNEFIDLGILRLISRGVKGNCCSVYSYVCSESS
                     NAEIVTKNGDIFKNIEKYIDATINNNQKTMKYKHRNELFKNSNTNIYSRINQESIDKD
                     YDLIIHTGYETENETKKKELLKNNLNKKNLNKNTFENSDEDLTEDSTYKNPESIHEII
                     IRKLNSESGKNFKADLSITKALIDKRLKEGYSLEDFYKVIEAKVRRWRYTDMEIYIEP
                     TILFGYEFESYLNEESSLSACNYGDMCYEDMY"
BASE COUNT         4396 a         2217 c         1475 g         5122 t
ORIGIN      
        1 acctttttct ggattagcag agtgagctgg tttaccaaaa gtttctacta ctatttctcc
       61 tctacctctt tgacctatct ttaggtttaa ttctgaagat tcacctatta caacataatc
      121 aggttgaaca gccttactta tctctctagc tgaaacacct tcaaatattt cttcatgaac
      181 aacacctgct acatacaatt caccttcaaa gtctttgtta gtatcttctg caaagtatga
      241 aacagcagcc atcatagcac ttacttgccc cttcatatca gaagttcctc taccatatat
      301 tttcccatct actattctcg cttcaaaagg aggaacactc catttacttg catctggaac
      361 tggaactgta tctatatgtc cgtcaaataa tattttttta cctggtttat ttccttttat
      421 acatcctact atatttccat atctatctct tgaccaacta tcaaaaccta gtaaagcaaa
      481 attttcttct atagctttta ctacgttttc ttcttgacct gaataactag catttcttat
      541 taagtcttgg catacttcag ttaattgttt ttttcttgtt tcatttaaca ttttgatatt
      601 cccccatata atttttaatt tgttgacgtt ttattttgat atttcacttt tttaatttat
      661 tagcatttca cttaatctat tgatttttta tttctttata ttttatttat taaattttta
      721 tttagacttt attttaattt tatttggttt tatttaattt tatttagttt ttattttgaa
      781 tttttcattt gctttacttt tttatttgct ttactttttc atttatttat taagtttttc
      841 ttttccttaa gtcttttatt tactttggca ttcaccattc caaactatat ctctatattt
      901 atcagggtca gtatctcctt ctgtacttat taataatact ttagagttct catctagttt
      961 caaagcttct cttaagtctt ttaaatcttc actttcaagg attgaaacta atgctccagt
     1021 tgtaactgct ccagattctc cagagattac tttctcatct cctcttagag ggtttcctaa
     1081 tattctcata cctttagcag atacccagtc tggtgctgaa acgaatgctg atgaataatt
     1141 ttttaacact tcaaaaccta tagtgtttgg ttctccacaa gcaagccctg ccattatagt
     1201 tggcatatct cctccaacaa atcttggttt tccatctcca gcttttgctg atttatagta
     1261 acaatcagcc tcatcagcct ctactactac tgttattggg cattcatcat atattgaagc
     1321 aacataccct tgaactgcac cagcaagtga accaactcct gcttgaacaa atacgtgtgt
     1381 tggtctatct actccatatt cttttaattg ttctatagct tctttagcca tagttccata
     1441 tccttgcatt atccatgctg gtatttcttc atatccgtcc catgctgtat cttgaaccat
     1501 tactccatgg tcatcagctt ctgcataatc attagctaat cttactgcat catcatagtt
     1561 taagtctgtt atactagcat ctgcaccttc tcctttgatt ttatttaatc taaattcaga
     1621 agaacctttt ggcattaata caacagattt ttgacctaat ttattagctg tccaagcaac
     1681 tcctctacca tgatttccat ctgtggctgt aaagaaagtt atttctccta atttttcttt
     1741 tatttctttt gaagttaatt tatcataccc taactcagat atatctgtat ttaatctttg
     1801 agctaagtat ctacccatag agtaagaacc acctaaaact ttaaatgcat ttaaaccaaa
     1861 tctataagat tcgtctttaa catatattcc gcttacgcct agtgattttg ctaagttatc
     1921 taatttaact aaaggagtct tttcatattg tggaaagctt tcatgaaaag ctttagcctt
     1981 tgcaatctct tcatctttta aaaattcaat tcctttttct ttttcctctg atttaggtaa
     2041 gttatttaat ttccatttta tttctctcat ttattttata ttcccctttc aaatttttcg
     2101 gtgtcatttt aattttttat ctttgtgcct tatagcaaag caattaccgt gccaatatta
     2161 tttattctaa aaacaatttt tttatgttgg aattacatac ttttataaaa tttctttaaa
     2221 gtttataaat tttgagtgtg aaaaataaga tttataaaag aaaaaattct catattggga
     2281 aatttttatc aatacgagaa ttatcaattc gagaaatcgt ttatttgtct taattcttcc
     2341 tttattatgc tcttattttt tatatgtcta ttgttttgtc agatatatta ctacttttat
     2401 tttctataaa tatatggcta taaccttctt acgcataaac tctataaatt tatatacttt
     2461 atatacttta tattctttat tacccaaagc ctccaaaatg gcttaaatct tttcatagct
     2521 tcttacagaa gtttacatgg tcatcccccc aataagcaaa agtgcaatat gtgtaccatt
     2581 gtgccctact tgcgactatt gagctgtcga aattcgcaac ttcgcattta tctgatgaaa
     2641 tgcactatac aaaatattca taacatccac aacagagtta aagttaccta ttcatattca
     2701 ctgtaaaaat ttcttccaca ggaaatgtaa ttttaaccaa tcctattgag acaaactcac
     2761 cacttatttt tttgaactca cgcagtaata catcacttgc ccgaaaaaaa gcctcacttt
     2821 catcatctcc agtaagtgct attgtgcaat ggggtaccca acgattgggg tgtaccattc
     2881 ccaaccattt gaatcaaaat cttgtagtac ctcatataat tctctctgaa actgatacat
     2941 actgctattc attataggtg atacaaacac cgttttagta tcattaaaca tgcctactga
     3001 accaatgtaa gcaggtatta ccttatggtt ttgtgcaaac ccctttagct tgtctatgca
     3061 acaagcctca tccacatcat tgaaacaagc caaagtaaga tgaggtcttg ttttccattc
     3121 taagtacttc gtactaattt tttcatcagc gattttttta gataaatcaa aaagttgctt
     3181 ttccgttttt ttatcataat atagctctat cgcatattgc ataataacac ctccaccata
     3241 aagtaaaaat acctatctat aaaaacacac aaatacctat cattttaata ttatattact
     3301 actgttcctc ttccaatttt ctatatagtg tagccaaacc tatgcctaag tcttttgcta
     3361 tcttcttctt cgtctttgta tctcttccat acttattcaa caacttgttt atgtaagtca
     3421 actctaattc tttaattgaa agtatccttt cttgattctc tactatccct ccaactatat
     3481 catcttcaaa tattatattt acatccttat ttttataaat atttccatta atattataat
     3541 agtttaaaat atctaatgga agcatatctt tatatattat tccatcatct ccaaccaaat
     3601 tcatcatata ttcaactgca ttttccagtt cccttatatt tcccggccaa ttatagttaa
     3661 tcaacatatt cattacattt tcttctattt tatttaactg aatacctaat tctaaagaat
     3721 atttagaagc aaacttcatc attattgcct caatatcatc ctttctttcc ctaagaggtg
     3781 gcaaatttat aggaattaca ttcaatctat agtataaatc atccctaaac ttaccttcat
     3841 ttactaattt caataagttt ttatttgtag cagcaatcac tcttatatct aaatctataa
     3901 gtttatttga cccaattctc gcaaattttc tctcttgtaa aactctaagt aactttgctt
     3961 gtaaaggcat tgacaaatct cctatttcat caagaaatat aacaccttca tttgcaagtt
     4021 caaatttacc tactttacct ccactactag ctccactaaa tgcacctttt atatagccaa
     4081 atagctcact ttcaaggaga ttttctggaa tagcagcaca gtttatcgca ataaatggct
     4141 tgttccatct gctaccttca gcatgtatag ccctagcaat tagctcttta cccgtaccac
     4201 tttctccagt tataagtaca gttgaatttg attttgctaa tttcttagtt ctctctttta
     4261 cttttaacat tgcttctgaa tttccaatta tagactctat atcactattt ccccagccac
     4321 tattcacttt tccatgtcct ttatgattga tatatgtctt gtcaaatatt attattttgt
     4381 cgtattgatt tatatatgga tataccggaa ttattttggc atttatatta taaattttgt
     4441 tatcaatatc aatttctagt aattcatgtc catttgagga agattcaaca gatactatat
     4501 ttactatatt ttctattata tttttatcta tatctaattt ttttagagct atattgttta
     4561 tataagatat tttgctattt atgtctagta ttataacgcc tttttcaaca ttttcaatga
     4621 tttctatcat catatccatt tttctactgc tattttttga cgcttcatgt tcttctattt
     4681 tcatagaaat taaatcgcat atatgattga caaattttaa ataattatct atttttgcac
     4741 ttatttctac ttttttagtt ttatctgtac taatagcacc aattactcct atagttcgat
     4801 tattatattt tattggaact gctattatta ctttatttag gcatttcatg taatgggaac
     4861 attctataca aagctgatta tcacatatat ccaatacgac taattcctga cctgtctgta
     4921 taacattgtc ataaataaat ccttctgtaa caacgctttc atttatcttt gatttataca
     4981 gacctgtacc actaattctt attaatctat cgtcaactat ttcaatatct acatctaaca
     5041 cactagctac tgtttcagtg tattttatca tttcttgttg aaattctttt aatttactta
     5101 acatagtttc ccccattaga atttaattta ccaaattatc agtttagcta tattttaaca
     5161 tatagtcact ctttaaaata tatataaaac attacttatt tatatatttg cgttaaaaca
     5221 gacaatatta atttataaaa agtttgtttt atatagtaac cttaattata atatgcagac
     5281 aataaaataa atcacaatac aataataaaa aaaagctttc ctcatataac aaatatattt
     5341 ttgacataaa aatatacatt atatgagaaa agctcaatag ttaaatattt agatagattt
     5401 ttatatagac ttttttaata taaacttttt taattgtaga ctttttttat tagtttttaa
     5461 tttttattag ttttgttatt ggttttattg ttataatact tataatactg ccattgcttc
     5521 aatttctaaa actgcatctt ttggtaattg agcaacttgt acgcatgacc ttgctggtgg
     5581 attttcttta aaatatgtag catatacttc attaatatca ttaaaatcct ttaaatcttt
     5641 tacgaatact gtagtcttaa atattttatc catacttgta ccagcttgtt ctaatattgc
     5701 ttttacatta tctaaagaag cttttgttgc ttcttttggg tcagatacta actcgccagt
     5761 cttcatatct attggaagtt gacctgatgt agaaactatg tttccaactt gaattgcttg
     5821 agaatatggt ccaattgctc ctggagcttt ctctgtagct attactactt ttgacatgac
     5881 ttcacctcct attattaagt tatatttaca ggaaaataaa atttcctaaa caccaatatt
     5941 aatacgaacc tttagattaa atttcaaatc taaaacttca aataatcaat acttagatga
     6001 ttatgtttat ttgtgtcttg ttaactttta ttaaagcaat taacgtgcca aactttattt
     6061 atatataaaa ttttgtgaag tcattcaaaa tagttacttc tgaatttttt cgtaaaaaaa
     6121 cgctctaaaa ttactttttt atgatgtcaa tttttttctc atatctagaa atttaccaac
     6181 atgagaattc ttgttttgag aatttacaga ttaaagttat tttttaacct taaattctga
     6241 acctcccatg actaaagtca cagggttcct gcttcataga attttgcata atcaagttat
     6301 tacatatgaa agaactatag ttgacaatcc cactattata aataataaaa cctttaccca
     6361 taccttattt accctagtta tcatagagcc tattgaaaat ataacagcta aatataataa
     6421 taaccaaaaa attatcaata tctaccactg ttgctgaaaa tccaaaaaac taccttttga
     6481 tagtcttact taacaatcta attaatctca taataaattt acaacttata aataaattgt
     6541 tatattggcc gtatctcaac aactttctcc tttcctagtt ttaaatcttt tatagaattg
     6601 taccacgtct aagtaaattt tcatactatt aagtcttatt tctaataaat cattagctct
     6661 cattctatgc ccaataatgt aagattgcag taattttatt gattttcata gttttattta
     6721 atatacttac attaattatt ttcatatgaa attattattt atataggggg atttttttaa
     6781 tatgagcaaa agtgaattaa cagcagaaac aacagaagaa atgttagaag tactaagtgg
     6841 taaagattat gatattgcat gtcatttaca tgaacttggt aaatcattag attgtaaaat
     6901 tgaaccaaaa acaggtgctc gttcttacaa aatagtatat tcaactaaga aaccaaaacg
     6961 tagcttattt actattgaat gtaatgaaaa aaaatggaga gttaaagcaa atctttttca
     7021 tctaaataca tataaagatg ctgtggagga atgctctaaa actattaaag atagtataac
     7081 taaaactcgt acttgtaaga aatgtaattc aaagtgtatt ggaggttctt cgtttgaatt
     7141 agatggaaag tcttacctga cttgcatagg aagtggtcat tattttgcaa atatggaaga
     7201 aatggattgg aaaaacctag aaaaattaat tactaaagaa aataatatta tgcaggaatc
     7261 tgtatagttt tttaagggta attattaaaa tatatgagta acaacagata cttaaatagg
     7321 cataaaaaaa taaagggtat aataaggata tagaataaaa ataaatgata tagcacaaag
     7381 aggctgtctc aaaatacaga taagcctctt tttattgttg aaatgataag aatatattca
     7441 attttataca attttataca aacatactac gccaacttac ccatttcata tgcctgtttc
     7501 attgccttac ttcccttaat atcacctaca ttccaagcac ctacaccata tactatacct
     7561 ttttccttag aaccatttag acatgatgta aatcctctaa atccttctat tgttctttcc
     7621 atatcagcca cattttcatc tgctgctgta acgataaaat agaaatcttt atcacaaatt
     7681 tcagtatatc tagagcaagt tctatcaatc atagttttca tttgtgcatt catagtataa
     7741 aaataaactg gagtcgctaa aacaatcaca tctgccccaa tcattttttc aagaatttct
     7801 cccatgtcat ctgtctgtga acaatttgct tctttttcat aacaagcacc acatccagta
     7861 caatagttaa tatctttatc ttttaaaaat atcttctcca cttgattttt agattctaat
     7921 accccttgta taaactggtc acacaataaa tcagaatttc caccttttct aggactagct
     7981 gatactatta atacattttt actcataaga tactgcctcc attttcttat ttctactgct
     8041 ctttaatact ttttctttct caataatacc ttcttcatat ctatcaatct taaaatttaa
     8101 tcgttccaaa gttttttgca tttcttctat tctttttgct agttggtttc tctgctctat
     8161 taaaagttct tttctagctt tgatagtact gctcccttgt tgaaacatag ctacatactc
     8221 aattaatgtc tcaatagaaa gacctgcacc tctcatacac tttatgaaat taacccactc
     8281 acaatcttct tctgtaaaag acctaattcc acttttattt ctatttacag gtggtattaa
     8341 accaattcgt tcataatatc gtaaagtatc cgctgaaagc tcgtattttt tactaacttc
     8401 tgaaatcgtc atacatatct tctcctcttg atattgtata gattgaatta tttctcaaag
     8461 gtttctgcta cattctttaa agcatctata attttaatgt gttgaggaca gctttcttca
     8521 cattgtttac attcaataca atctgaggcc tttccataag ttttagtata attattatag
     8581 tagaccatct gtgttgaaaa tccagtatta agagcttgtt tttctgcatt gtacaacgca
     8641 aaatagtttg gaatagcgat attttttgga cagccttcta cacaatactg acaggcagta
     8701 caaggaatta aaattgattc gttgataatc tctactgctt catctataat attatattcc
     8761 tcttgtacga atggtttgaa attttgcata tatcctgtat tgtctaataa ttgttccata
     8821 tttgacatcc cacttaagac catcataaca ttatctttac ttgctgcaaa acgtattgcc
     8881 caagatgaaa ttgacatatc tggattgtag tcattcaata atttctctgc tttttctggt
     8941 atctttgcaa gtgtacctcc ttttactggc tccataacaa ttacaggctt attatgcttc
     9001 tctgccacct cgtagcattt tctagattgt atgctttcat tatcccaatc taaataatta
     9061 atctgtaatt gcacgaagtc tacttctgga tgctcactca agactttatc caataattct
     9121 gcactatcat gaaaagaaaa accaatattc tttatttttc cttctttttt cttttcttca
     9181 ataaacttaa agctatcaaa ttttttagct acttcgtaat gtgacacgcc tatattgtgt
     9241 aaaagatagt agtcaaaata gtctacacca catttttcta attgctcatt aaaaatggtt
     9301 tcttgctctt cttttttctt taaagccatt aatggaagtt ttgtagctat agtaaaactc
     9361 tctcttttgt gtctttttac tagagattct ctaagtgcta tctcactttt tcccatgtga
     9421 tacacatagg ccgtatcaaa atatgtaaat cccctttcta gaaaagtgtc taccatttta
     9481 tttaaatgtt ccatatcaat agttgtaaca tcattttcat cttttattgg taaacgcatt
     9541 aatccaaatc ctaatttttt tgcctccatt gctttacttc cccctttaaa acttgtttgt
     9601 tttttctctc tactttaact attcttacaa gtatataatt cttgtataat tatactttac
     9661 tacttggagt taacttcaag tcaatagatt tttttatttt taagacaaat attataaaaa
     9721 tacagttctt taatattaat ccattacatg tttctttcac cctccaattc tcaaacttca
     9781 caaactccaa catgtaaaga aatatatact ttaagaaatt cagtccatta atattactac
     9841 aaatactttt tagatatgtt taaaaagaaa tttcacctaa aagatttaga aaagatttag
     9901 aaataggtct tgtgtctatt ttaaaatata taaaacagtg gtaaagactt atttataaga
     9961 taaaaaatga atagtgtata ccctttttaa aaagtttttt cctttttata tttgtgatac
    10021 aatgttaaat ataaaaagga aaattttgta ttaaagaaat cttatttgtt gattttgtaa
    10081 cttcaaactc agcatcaatt tgattttaag attatatttt attaagtatg ctaaataaca
    10141 aatttttaaa gattgtgagg taaaaatatg aataagaaaa atttagctat attaatatca
    10201 ggtatgatgt ttttttctag ttcttcagca atttttgcag acaatactac aaagcaagaa
    10261 agattaatag gtaaaacaag atatgaaaca gcagtagaag taagtaaatt gggatgggtt
    10321 caatcaaaaa cagccattat agttaatggt aattctattc aaagtgcgct ttgtgcaaat
    10381 ccatttgcta agctaaagaa tgcacctata cttttagtaa acaacaacag tattgaaaat
    10441 tcaactaagg ctgagcttaa aaggcttggt gtagataatg tgtacattgt agatagtgga
    10501 aatagcattt cttctaaagt agaaaatgaa ataaaatcac ttaacataaa aataaataaa
    10561 atagtaggaa acaatatata tgaaatgtct acaaatgtac ttaaagaaat agataagata
    10621 aaaaaggtag aaaatgttgc agttgtaaat ggactaaagg gactatctga tgcagttagt
    10681 atagcttctc cagcagcaat acataatatg cctataatat tagtatcaga tgaaaacagc
    10741 aatgatagta gtataaattt cttaaacaat aggtctataa aaaaatctta catagttgga
    10801 ggaaataaga ttttatcaga caatacagta aagaaatatc caaacagaga aagaatcagt
    10861 ggttcagata gaaacaatac taatgcaaaa attattgata aattttataa agataaaaaa
    10921 ataaataatg tatttgtagc taaagatggt attaaccaag aaaatgcatt agtagattca
    10981 ttatcaatag gagtactagc tgctaaagaa aattctccta taatgatatc taatggtagc
    11041 cttgtaaata gtcaaaaaga atttattaaa aataaagata tagatgaaat aacacaagta
    11101 ggaggaggat ataatcaaaa agcatttgaa gatattttaa aactaaaaaa tgtgaacata
    11161 agaaataaaa ctaaaaaaat atctttaata caggctaaag aaatattaaa acaagctctt
    11221 ccaggaagca atattttagt ttgttattat gatgaagatg ataatgaata tgactgcaaa
    11281 gttataaagg ataatatcct atatgagata gaaattgatg cctactctgg taaaataata
    11341 gaaattgaaa aagatgatga cttaaatgat aataatttag gtacacaaaa tatatcattg
    11401 gataaagcta aatctatagc attagatgcc attcctaatg gtaatttaat ctattgtaaa
    11461 tatgatgctg aagataacga atatgaggct aaagtaataa aaaataagaa aacatatgaa
    11521 ttagagataa gtgcatttga tggaagaatt gtagatattg acatggacaa tgattctgat
    11581 gatgactttg atgataatga taagaataac tctaatacta atgacaacag ttctattatt
    11641 agtgctgaaa aagctaaatc aataatgcta gataaagtgc ctggtgggaa aatagtaaaa
    11701 tttaagtatg atgaagatga caaagaatat gaaggtgaaa taataaaagg aaaaactgaa
    11761 tatgagataa caattaatgc aattactggt aaaatattag aatttgaaat agatgattaa
    11821 taaaaaatat atattctatt aattattaca agcaataaag ccataaatat tgaaattttc
    11881 aatatttatg gctttatttt atttttttaa tttaaatcca cattatgcca ttatatatat
    11941 ctatgcactc ttgtaaaatg ggttatattt ctccagtata ttaaaaaaag tgagtgtctc
    12001 aaaataaatc ttctaactca taaagcactc tcctttgtat taaatcaaat atattttcat
    12061 catttaaaca attaagaaga gacttctctc tattttgaga caactccatt ttaatacact
    12121 atttatacat aaacatattt taaacaacta aaaagcaact aatatcttaa taaatatctc
    12181 aatacatatc ttcataacac atatcaccat aattacaagc agataaagaa ctctcctcat
    12241 ttaaatacga ctcaaattca tacccaaaca gaattgttgg ttctatatat atttccatat
    12301 ctgtatatct ccatcttctt acctttgcct ctataacctt gtaaaaatct tctaaactat
    12361 acccttcttt taatctttta tctataagtg ccttagttat actcaaatca gctttaaaat
    12421 tttttcctga ttcactattt aattttctta taataatttc atgtatgctt tctggatttt
    12481 tgtaagtaga atcttcagtc aaatcttcat ccgaattttc aaaggtattt ttatttaaat
    12541 tctttttatt taaattattt tttaataatt cttttttctt tgtctcattt tctgtttcat
    12601 acccagtatg aattatcaaa tcataatctt tgtctatact ctcttgattt atacggctgt
    12661 atatgttggt attactgttc ttaaacaact catttctatg tttatatttc attgtttttt
    12721 ggttattgtt aatagttgca tcaatgtatt tctctatatt tttgaaaata tcaccattct
    12781 ttgttacaat ttctgcatta ctactttcag aacaaacata actatataca gaacaacaat
    12841 ttccctttac tcccctactt attaatctca atatacctaa atctataaat tcatttacaa
    12901 gtctacttat tgtagatata gatacatcaa ggtcagatgc agctttcctt actgtcatca
    12961 taaattgtcc tcttggcaga gttcctttta atctttgtat attttctttt ctcattacat
    13021 agtgattaaa ccttattcta tttacatctc ttttttcatt aaaatataat actgctgaat
    13081 ttaattgata aaaactatta ctcatattcc cactcctcca atttaaatag ataatttgtt
    13141 gtaaagtttt tagatttttt aataacttat taaatgatta tttttattaa aaagtattct
    13201 ttgatatttt
//
LOCUS       DAELLV010000097        13168 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.097, whole genome shotgun sequence.
ACCESSION   DAELLV010000097 DAELLV010000000
VERSION     DAELLV010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13168
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.097"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..681
                     /locus_tag="KPD58_002118"
     CDS             <1..681
                     /locus_tag="KPD58_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG0265882.1"
                     /translation="RILNATISKEPSGRYYVSLCCTDVDIEAFENTNNHIGLDLGIKE
                     FCISSCGEFIENPKYLKKSLNKLAKLQRELSRKTIGSLNRNKARLEVAGLQEHIANQR
                     KDFLQKLSTKLIKENDIICIEDLQVKNMIRNRKLSRLISDVSWSEFIRQLEYKANWHG
                     RQIVKVGKFFASSQICNKCGYKNEEVKDLNIREWICPSCNETHDRDINASINILKEGL
                     RLITTQNK"
     gene            1236..2642
                     /locus_tag="KPD58_002119"
     CDS             1236..2642
                     /locus_tag="KPD58_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBG0265883.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLDFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNIVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEVLDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            2670..3518
                     /locus_tag="KPD58_002120"
     CDS             2670..3518
                     /locus_tag="KPD58_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG0265884.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            4179..4871
                     /locus_tag="KPD58_002121"
     CDS             4179..4871
                     /locus_tag="KPD58_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0265885.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLAELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCAKHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            4868..5803
                     /locus_tag="KPD58_002122"
     CDS             4868..5803
                     /locus_tag="KPD58_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0265886.1"
                     /translation="MKMDEQTIILFLLLLSIGLLFLSIFAFSKLGHIYKRLKDIEEIL
                     ADVQIGDENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIEQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVEDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQISVESEIGKYTIFTVSLPLI"
     gene            5951..6874
                     /locus_tag="KPD58_002123"
     CDS             5951..6874
                     /locus_tag="KPD58_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0265887.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRTFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            6858..7628
                     /locus_tag="KPD58_002124"
     CDS             6858..7628
                     /locus_tag="KPD58_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0265888.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVQKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPILSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            7638..8420
                     /locus_tag="KPD58_002125"
     CDS             7638..8420
                     /locus_tag="KPD58_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0265889.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVTLVVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            8796..9332
                     /locus_tag="KPD58_002126"
     CDS             8796..9332
                     /locus_tag="KPD58_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBG0265890.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            9364..9753
                     /locus_tag="KPD58_002127"
     CDS             9364..9753
                     /locus_tag="KPD58_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBG0265891.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            9790..10176
                     /locus_tag="KPD58_002128"
     CDS             9790..10176
                     /locus_tag="KPD58_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HBG0265892.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            10303..11745
                     /locus_tag="KPD58_002129"
     CDS             10303..11745
                     /locus_tag="KPD58_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBG0265893.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVEDESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFART
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            12042..12857
                     /locus_tag="KPD58_002130"
     CDS             12042..12857
                     /locus_tag="KPD58_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0265894.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTIELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINDFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIITGCSHSGICNIVEYAKKVCRDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEIRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
BASE COUNT         4815 a         1601 c         2463 g         4289 t
ORIGIN      
        1 agaatactta atgctactat atctaaagag ccaagtggta gatattatgt atcattatgc
       61 tgtactgatg tagatattga agcatttgaa aatactaata atcatatagg tttagattta
      121 ggaattaaag aattttgtat ctcaagttgt ggagaattta tagaaaatcc taagtatctt
      181 aagaagtcat taaacaagct tgctaaatta caaagagaat tatcgagaaa aacaatcggt
      241 agtttaaata gaaataaagc aagattagaa gttgcaggac ttcaagaaca tatagctaat
      301 caaagaaagg attttttaca aaaactatct actaaattaa taaaagagaa tgatattatt
      361 tgcatagaag atttacaagt aaagaatatg attagaaatc gtaaactatc tcgcttaatt
      421 tctgatgtgt catggtcaga gtttattcgt caattagaat acaaggctaa ttggcatggt
      481 agacaaattg taaaggtggg taaattcttt gcaagttcac aaatatgcaa taaatgtggg
      541 tataaaaacg aggaagttaa ggatttaaat ataagagaat ggatttgtcc tagttgtaat
      601 gaaactcacg atagagatat aaatgcaagt ataaatatat taaaagaagg attaagacta
      661 ataacaactc aaaataaata acttatgtaa gaaccgtagg aactacgggg atagcctgtg
      721 gagaattagt aagacatatt ttagtatgca aaattctgtg aagcaggaac cctgtgactt
      781 tagtcatggg aggttcagtt tgggcaagta gtttcttgta aatctagtta tgtttcaacc
      841 aatgataata taatttcatt agtattagct ggtaattaag ttaatttgac tagagataag
      901 taaattgatt taaaaggaaa taagtaaatc aacaattaaa tacatctaaa agtaagttta
      961 ttgcaatgaa ttgttaaaga aaataattaa tatggagtaa atctattgca atgaagtaac
     1021 aaagaaaata attaatatag agtaagttta ttgtaatgaa ttgttaaaga aaagagttaa
     1081 tatagagtaa gtttattgta atgaattgtt aaagaaaata attaatatag ggtaaatcta
     1141 tctcaatgaa ataacaaaga aaagagttaa tatagagtga acttattgca atgaatcaac
     1201 aaagaaaata atcaatataa aaaggagaaa tatttatgaa tacaggattt ccaaaagact
     1261 ttttatgggg agcatcatct tctgcatttc aagtggaagg agcttgggat aaagacaata
     1321 aaggaaaaac cgtagccgac tataattctt ttaaaaagtc tcatttacag gcagatacta
     1381 aagtcgcatc agacttttac cataattatg aagaagatat tgaattaatg aaagaattag
     1441 ggatgaaaac atatcgtttt tcaatttcat gggcaagaat aataccagat ggtgaaggag
     1501 agataaacca gaaaggttta gacttttata ataagataat agacaaacta atagagtgtg
     1561 atattgagcc attcgtaact ttataccatt ttgaccttcc atttaaatta gtagagaaat
     1621 ataatggttg ggaaagtaga gaaactgtct atgcttttga aagatttgct aaaatatgct
     1681 ttaagcattt tggagatagg gtaaagtatt ggcaacctca taatgaacag aatttaattg
     1741 ttagagtgga ggagagaatt aatatatatg atgaaacaga ttcatggaag atagataaaa
     1801 tgcgtgctca gatggattat aatctttgct tagcacatgc attagcagtc aatgcatgtc
     1861 atgagatgat aaaagaaagt aaaattggag cagcagtatc atcatctgta acatatcctc
     1921 ttacttctaa gccagaagat gtatatgcag ctagaatgaa tgataatttt aaagtgtact
     1981 atatgcttga tatgcatcat tatggagaat atccagggta ttatatgaag tatcttgaaa
     2041 aaagaaatat agttcctcac atggaagatg gggataaaga gatacttaaa aaagctaaaa
     2101 tggattttat agctgtaaat tattatagga caaattgtgc agaagcttta cctgaagatt
     2161 cacaacatcc atttggatta agagaaggaa ctgttgattt tagtatgtat ggactattta
     2221 aaatgtcaat gaatcctaac ttagaggcct ctgagtatgg agcagcaata gacccatcag
     2281 gtcttagagt tgccttaaat gaatattggc aaagatatca cttacctgtt ataataacag
     2341 aaaatggtct aggagctaaa gatatattag aagatggaaa aattcatgat gactatcgta
     2401 tagactattt aagaagtcat ataaatgcat gtaaactagc tattgaagat ggtgtagaga
     2461 tgattggata ttgtccatgg tcatttacag accttttaag ttcttctcaa ggttttaata
     2521 agcgttatgg acttgtttat ataaatagaa ctgaccatga agttttagat ttaaaaagaa
     2581 tcaagaaaga tagtttttac tggtataaag aagttataga gaacaatgga atagtaaaat
     2641 aaacataaat atatattgag ggttttagta tgtacattac aaaggtatta aataattcgt
     2701 tgctacttgc aaaggatgat aatgatgaag aaataatatt gatggggaaa ggcattggcc
     2761 ataattataa ggccgggtct gagttaaata aaaaagatat agaaaagatt tttgttcttc
     2821 atgatgaaaa tataaaaaag agcattatac aattggcaac agaaatagat gcagaatatt
     2881 ttggtatagc tcaaatgata attgcatatg gaatagaaaa atataactta aaacttatga
     2941 accatatata tttggcctta actgaccaca ttgcctttgc agtgagacgt tttaaagaag
     3001 gtataatgat tgaaaatcac tatttgttcg aaatcaagga atttaatcca aaagagtatg
     3061 atataggaaa atatgctatt actgttttta aggaggttct tggtttagac ctaccagaag
     3121 aagagattgg taatatagca ggccatttta taaatgcaca acaagataat ccttatagtg
     3181 atagaaataa gagaagtgct aaaattgtaa atgctatttt gcaaatagtt cattatcact
     3241 tttctattgt atataataaa gagtcatttt attataggag atttgtaatg catctaaagg
     3301 ccttttcaca aagatttctg gctaatgaac catcaagaga aaaaatagat tttatatatg
     3361 agcaagttca aaaaaattgt aaggaagagt atgaatgtgt gaaaaaggta ggagcttata
     3421 tgtataagga atactccaga gaattaccaa gacaagagga gttatatttg atgattcata
     3481 ttcataagat tttaggagaa ttatgcgaaa atagttagaa tatcttaata tgctagtaat
     3541 attataaaaa attaaaaatt aactacactt taaatatgaa taattgcatt acttaagggg
     3601 gttgtcttaa aattgatttt tttaattaaa tttgaagctc aactcctttt ttaaaccttt
     3661 atatcattac aactttaatg tttatacgcc tgtacttcag atgtatatct actttttaca
     3721 tttctcaaca tgcaaatcta ccattttttg cagtatgtta attgaaataa accattgagt
     3781 taaccctaaa tttattctgg acttatagga atagttccaa agatacttta aaatattttc
     3841 tggaataatt gcttatttca catatgtttt attgtgatgt tttgatttaa taaattcttc
     3901 aactctgtat atagtaaatg tattatcatg tttgttttat tcaagcacac ttataatacg
     3961 cttgattttt tcacaatgat gaaatatact aatgggtgtt ataagtataa gaatagcatt
     4021 acacttgaag aagcaagtga taaatgaggt ataaatcctc atatgataaa ctactatttc
     4081 attgaggagc atattctaaa tgatgtaaag atactacaat atgactaaat tcctaatgat
     4141 aaaaaagtct acgaatgagt gtagaaaggg gaactaagat gaataaagtt ttaattatag
     4201 atgatgataa agatctttgt attttgttaa aaaagagtat ctcaattgaa cagattgaag
     4261 caaactattg ccattctggg agagatggac tggctgaact ttcaaaacaa gattatcagt
     4321 tagtagttct agacgtgatg atgccaggta tggatggttt tgaaactatg gaacaaatac
     4381 gaaaatatag cagtattccc atcttaatgc tgacatcaaa aagcgataat tacagcaaag
     4441 tgcatggatt gcgagctggt gcagatgact atctaaccaa accatttgaa attacagaat
     4501 ttactgctcg tgtactgtct ttaatccgac gttacacccg ttttaatcca aaaaatgagg
     4561 agccacagca actttctttc aaaggtttgt tgattgacct tgaccagcat acagtgacta
     4621 ccaatggcga acctatgttg cttccaccca aggaatttgg tgtgcttctt tactgtgcaa
     4681 agcatcaggg taagattttg acaaaacagc agatttatga agccgtgtgg ggtgagccat
     4741 atgtatatga tgacagcaat atcatggcta ttatcagtcg cctaagaaaa aaaattgaac
     4801 tagacccatc atctcccatc tatttgcaaa caataaaagg aattgggtat caattcaaca
     4861 cggaggtatg aaaatggacg aacaaacgat aattcttttc ttactcttgc tgtcgattgg
     4921 tctacttttt ctttccatat ttgctttttc caagcttggg catatttaca agcgcctaaa
     4981 agatatagag gagatactgg ctgatgtaca aattggagac gaaaatcgta aaatactaat
     5041 taagccatgt gatgttatgg ctccattggt ttatcagctt aatgagattg tctacgatta
     5101 tgaaaataag ttactttctc tgaaaaagtc ggataaagct agcaaacaac ttatgacaag
     5161 tttatctcat gatgttcgta caccattaac aactctgatt ggttatctgg atgctgtaca
     5221 cagtggtata gttatcgaac aagaacgaga ggaatacctt gaaatcgcta ggcgtagagc
     5281 ctacgacctc aaggattaca ttgatgttct ttttgactgg tttcggttga actctgatga
     5341 atttacgctg tcaatagaat ctgtagaaat tgcagagttg tcacgcaata ttttgaaaga
     5401 ctggattcct atattccatg aaaagaaact gaattttgaa attgatattc cagaaaatcg
     5461 actaatggta aacctcgacc cagatggata ttcccgtgtg gtcaataatc tggttcaaaa
     5521 tgttcttgct cacagtaagg ctcggcaaat caagattacc atgagtgagg attccagaat
     5581 ggtattactc cgtgttgagg atgatggtgt tgggattgcc agagaaaatt taccacatat
     5641 ttttgagcga ctgtataagt gtgataaagg acgttcagaa aaaggaagtg gtcttggtct
     5701 atctattgtc agtcaaatgg tggagcgaat gggtggtcag atttccgtag aaagtgagat
     5761 tggaaagtat accatattta cagtgagctt accattaata taaaattgtt tttcccccat
     5821 gtgacatgta tacacagggg gattttttgt aagttataaa gttgcaaggt taatacaagg
     5881 ttgttgcaag gttaatgtaa gattgaggtg gtatgataac tttaataaac aaaggaggct
     5941 tttattattt atgaataatt taattattga aacaaaaaat ctcactaagc aatatggtgc
     6001 tcaaaagagc gtttctaatt tgaatattca cgttcagaaa ggtcgcattt acggattgct
     6061 aggaagaaac ggtgcgggta agacgacaac tatgaaaatg ttattgggtc taacaaaacc
     6121 aactactgga gaaattaaaa tatttggtca ggacattcac aataacgaga agaagcttct
     6181 tcctcgaatt ggatgtttga ttgaatcacc agggttttat cctaatctga caggaacaga
     6241 aaatctcaaa atctttgcaa ggcttagagg aataccaaaa aaaaatgcag tagaatcagc
     6301 actggatgta gtcggtttgc caaacaatga caaaaagtta ttttctcaat actctcttgg
     6361 tatgaaacag aggttggcga ttgctcttgc cattatgcat gacccacaac tgttgatttt
     6421 ggacgagcct atcaatgggc ttgatcctat tggtattgca gaagtaagaa catttattcg
     6481 tgatctctgc acagagcgag gtaaaactat tttgatttct agtcacattc tttctgaaat
     6541 tgccttgttg gcagatgaca ttggcattat tgaccatggt attttgttgg aggaagaaag
     6601 tcttgcccaa ttagaagaaa aaaacagtaa atacatacat tttgctgtat ctgatgcagc
     6661 acaggcagca aggcttctgg aaacccaact taacctgaaa aatttcaaag tagcagataa
     6721 ttacaatatt atgctatatg atacagatgt ttctgttgca gaaataaatc gtgctttcat
     6781 tctaaacaac attgaggtat cagaatctca tttatgcgaa gatactcttg aagattactt
     6841 caagaaagta acagggggtg aaggaattgc ttgatataat ttcatgtgaa ttttcaaaac
     6901 tcaagcggtt aaaatttata ctgatttcta ttttaggtgc ttgcttattt ccaattcccg
     6961 caacaatttt gattgcaaag gataacttgc cctttgagca attgttcaaa ttagttgtca
     7021 attttggata ttttttgcta ttaccaattg tattaagtat tgttgctagc caactatttt
     7081 ttatagaaag agataatgat gtattaaaaa atcttgcaac agtgccagtg caaaaaggaa
     7141 aactggctct ggcaaaattg gctgtgttgc tgtttatatc attgttctac tctgttgcag
     7201 ggttaggagc tactatcatt ggaggtttca ttgttggaat agtagagggg gttgctgtaa
     7261 aattaggaat gagcattgct tttggaatta tgttttttgt tactgtactt cctgttgtgg
     7321 tactcattgt atatttcaat cgcagctata ttttttctat tatactttct tttgtatatg
     7381 ctattttcaa ttttagtata tccttaaata ttatcaattt tgaaccaaat aacccaatat
     7441 taagtgtatt acctgctcct gttataatgc gttggtggat ggcattttgg ggagatccca
     7501 ctggcgaata cacggcactt agacagccat atttgctctc aactcctgct tgtgtaggta
     7561 ttttatgttt gataaccatt atagcagtcc tgctgatttg tatgatatac aaaaagcagg
     7621 agaattagga ggtatctatg cttgatataa ttactacaga atttcaaaaa ataaaaagat
     7681 ataatattct atggattggt gttgttgctg ttctgttttc agcactattg gcagtttttc
     7741 aggttgtaag ttcacacggc agtgaccctc taacgtatga gagctttgca aacggagtta
     7801 tctggaataa tttcagtctt agttttccat ttgggattac acttattgga ggatatctta
     7861 ttaaccgtga atatacagac caaaccttaa aaaacatcct aactgttcct atttcattac
     7921 gcaaattgct agtgggaaaa ttaattgcgg ttggaggaat tactgtattg ttaagtcttt
     7981 tcagcttctt atgcacattt attttggggt tgttattttg caatattaat atatctgtta
     8041 cactcgttgt aaaatcctta gttcagattt tgtctgttaa tctgtactgt tatgttgctg
     8101 ttttgccgat tattgcttat tttggtcgta aacaaaatgc atttctgacg ggtgtaggga
     8161 ttgcatttgt atacggtttt tgtggtgtgt ttgttgctgg aagaaatctg acagactttt
     8221 atccaatcac agcaggtctt ggtctagttg gatacaacaa tggtgctggt actgtttatc
     8281 agccacttat tggtgctatg actttgattg taattctaat cttgacaact attctacttg
     8341 tatttacacc taattatgat aaagttatga ccatatctaa gaaaaaaggg ataaagcata
     8401 aaaagtattc tcataaataa cataaatatt gtttctatta tttaagttgc tagacagtga
     8461 aaagaaaaaa tctgtcgtac agtggctata tgctcatgta ttcaagaagc tacagttttg
     8521 atttttcata actgtagctt ctttttgtat ttattaaatc acgactatca taatacttgc
     8581 aataggtata gcgacaagag agagtactgt catatgtgtt ttatatatta cattaagtat
     8641 attctaaaat atatgtttta aataatatat ttgaaaatag gaatatttaa ataaaaaaaa
     8701 taatattata tatagttgac aaaaattggc aaatgatata atatataaat atagaaatag
     8761 taatcattac tattattaaa agggaggaat taattatgaa cttaaaagga actaaaacag
     8821 aaaagaattt aatggcagga tttgcaggag agtcagaagc aagaaataaa tatacatact
     8881 acgcttcaaa agcaagaaaa gaaggttata atcaaatagc tgctatattt gaagaaactg
     8941 ctaacaatga aaaggaacat gctaaattat ggtttaaact tttacatgat ggtatgcctt
     9001 caacagagga aaatttaaag gatgcagcag caggtgaaaa ctatgaatgg actgatatgt
     9061 atgcaaaatt tgctaaagaa gctagagaag aaggatttga caagatagct tatttatttg
     9121 aagctgttgg aaaaatagag aaagagcatg aagaaagata cttaaagctt ttagaaaact
     9181 taaatgaagg aaaaatattt aaaagagatg aagaagttgt ttggcaatgt caaaactgtg
     9241 gtcatgtgta tgtaggtact gaagcaccag aaaaatgccc agtatgtgac catccaaaag
     9301 catattttaa tataaaagct gaaaattatt aaaaattatc attattaaaa aggaatgtta
     9361 actatgaaat tttctaaaca acgagaactg attttaaatg aaatattaaa taatccggtt
     9421 catcctactg cggattacct atatgaaaac ttaaaaaaag ataatccaaa tttaagctta
     9481 ggaactgtgt atagaaattt agctcagcta acggaacatg gctttataag aaaggttagt
     9541 attccaggat atccagatag atttgatggt cgaatagata atcactatca tatcatatgt
     9601 gaagtatgtg gagaggtata cgatttagaa tccgaagttc ttaataattt acaagaatta
     9661 atatctgagg agacagacat aaaaataaca tcttataaca taagttttaa agggatttgt
     9721 aataattgta aaaggtgtag ccaagtaggt tagttttcaa attctaaaaa taattaatga
     9781 ggtgagttta tgtgtagtga acaaaaattt tttatatgtg agacttgtgg aaaccttgta
     9841 ggaatgatac aaagtggtgg tgtacctatt ttctgttgtg gaaaacctat gaaggaactt
     9901 gttcctaata caacagatgc agcagttgaa aaacatgtac ctgtaattga agtggatgga
     9961 aataatgtta ctgttaaagt aagtagcaca actcatccaa tgactaaaga acatcatata
    10021 gcatgggtgt atcttatgac tgaacaaggt ggtcaacgca aatgtctagc agtagatgga
    10081 gaacctgtag ttaaatttgc tttgaatgat gatgataaag tgatttctgc ctatgcttac
    10141 tgtaatttac atggattatg gaaggctgaa ctgtaatatt taaataaatt gagtcaaaaa
    10201 ggaggctata aatgtatttt catacaaata gcctcctata aatttatata aattcaagtt
    10261 ttaattttgt ttaatgtcaa tataatataa ggagaaaata atatgtcaat ttataaatgt
    10321 agtgtttgtg gatatattta tgatgaatct aagaatgata aaacatggga tgaattaagt
    10381 gaagattggg aatgccctgt atgtacaaaa ggtcgtagtt attttggaaa gataagtact
    10441 gtttactatg aagaagatga aaagatagca gaagacatag ttgaagatga atctaaatta
    10501 aatactgaaa aagagggaga tttaaattac ttaagtacat atttaagaag agatgatgaa
    10561 gttgagaaac atatggatat aattcacgaa atggctgtta caggaaaatc tataatagaa
    10621 cctatgagaa ctaaactacc agtaatttct tgggatgata ttttaattat gggtgctcaa
    10681 ttaaatccat tacctttaaa tgagcatgat gaagtaaata caacaacaat aataggaaaa
    10741 aaagcaaaga aacctatgat tatagaaaat ccagtttata tttcccacat gtcatttgga
    10801 gctttgtcta aagaactaaa aatagcttta gcaaagggtg cagcccaaaa taagacagct
    10861 atgtgtagtg gtgaaggagg tatattacca gaagagaagg aagcttcata caagtatatt
    10921 tttgagtatg taccaaataa atatagtgta actgaagaaa atcttaaaaa ttctgatgct
    10981 atagagataa aaattggtca aggaacaaaa cctggtatgg gaggtcatct tcctggcgaa
    11041 aaagtaactg aagaaatagc aaaagtaaga aataagcctg taggtcaaga tgtaataagt
    11101 ccatcttgtt ttgaggaaat acaatccaaa gaggatttaa agaaacttgt agatgaactt
    11161 agagaagttt cagaagggcg tccaataggt gttaaaattt cagcaggtca tattgaaaaa
    11221 gatatggaat ttatagctta tgctaaacct gattttgtaa ctattgatgg tcgtggtgga
    11281 gctactggag caagtccaaa gttgcttaaa gatgcaactt caattccaac aatatttgct
    11341 ctttatcgtg ctagaaaata catagataca catggattag atatagattt ggttataaca
    11401 ggtgggcttc gtatatctac tgattttgct aaggcaattg ctatgggggc tgatgcagtt
    11461 gctattgcaa gttctgctct tatggcagcg gcgtgtcaac aatatcgtat atgtggttct
    11521 ggtaagtgtc ctgtaggagt agctactcag gatgaggaat tgagaaaaag attacatata
    11581 gaaaacagtg ctaatagagt ggcaaacttt ttaaatgtaa gtttagaaga acttaagact
    11641 tttgctagaa cttctggtca taaagatata catgatttaa gtgttgatga tttatatact
    11701 gttaactctg aaatatcaaa ttatacaaac atacagcatg tatagattga agtttgaaac
    11761 tattagattt tagatagcta taaaagtaaa tgataaatat attttataaa agagagtgtc
    11821 ttaaaatgaa ctttttagtt cataagtcac tctttttttt atgaagccaa atatatttat
    11881 cattttagca atgaaaaaga atcttatctc tttattttga gatagattcc ttctatttat
    11941 gaaaattaaa gatattaaat tgtaaaatat tctgtgatgt cgtactatta aactaaagat
    12001 tctgtacttt aaagtaaata aaaatttaag gagtgattat tatggaaaga ttgaagatac
    12061 aagttttagt cgacaataat acttatattg atagatactt tgttggagaa ccagcagtaa
    12121 gctattatat tgagattgat ggaaatagga ttttgtttga ctcagggtat tcagatgtat
    12181 tcatatcaaa cgctgaaaaa ttaaatatag atttaggaaa tttaactcat gttgtttttt
    12241 ctcatgggca taatgaccat acaagaggta tccagttttt agaaaatagg tatgatttat
    12301 ctacaattga acttatttca catcctaact gttttatacc tagaaaacat ggagagaaaa
    12361 gcataggagc tccattttct gctgaggaaa ttaaaaatat ttttatgtat aatcctaagg
    12421 ataagccatt taatctaagc aagaattgtg tgtttttagg tgaaatacct tctataaatg
    12481 attttgaaaa aagagctaaa attggtaaat gtaaaatagg agatttgtgg gaagatgatt
    12541 atgttttaga tgattctgct atagtttgta aaactgataa aggtattttt attattacag
    12601 gatgttctca tagtggaatt tgtaatatag tagagtatgc taaaaaggta tgtagagatg
    12661 atagagttat tggtatacta ggtggttttc atctttttga attgaataat aggttagata
    12721 gtacaataca atatttggat aaagaagaaa taagaatgtt gtacccttgt cactgtgttt
    12781 cttttaaggt aaaagcgaag atgaatgaaa tattgaatat aaatgaagtt ggagtagggc
    12841 tgaccataag tatatagtat ttgaaagtat ataagattta agttgaaggt taaaaagata
    12901 ttttctatag aaaatatctt ttttgttacg tttattttaa gagaaatcaa tatactttta
    12961 tatgaaatat tgtaaaaaaa tttattttta atgagaatat attttcatta ataaacaatt
    13021 ttattatgta ttaataatgt tacaataaat ataaataaat caaagttact tacattacag
    13081 tatatttttt cacaaaatat attgatttat tcttttaaag tttgataatt ccaatgaatg
    13141 taataaaaga agggatttta tactatat
//
LOCUS       DAELLV010000098        12880 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.098, whole genome shotgun sequence.
ACCESSION   DAELLV010000098 DAELLV010000000
VERSION     DAELLV010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12880
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.098"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            125..265
                     /locus_tag="KPD58_002131"
     CDS             125..265
                     /locus_tag="KPD58_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265895.1"
                     /translation="MEKSNTTVTKKEKIEDIGMKLEHLLSEDITFYELLLEILYKEKE
                     EN"
     gene            complement(262..633)
                     /locus_tag="KPD58_002132"
     CDS             complement(262..633)
                     /locus_tag="KPD58_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0265896.1"
                     /translation="MESLGDRIANLRKELDINQKELATKVGITEASLSRYENNLREPK
                     SEIIVRLAKALETSTDYLLGVNDNTKISKEDKLIIENLSVSEKTKKLLEKIYSLEKED
                     REAIEKMIDNAYLKRFLKEEN"
     gene            1182..1439
                     /locus_tag="KPD58_002133"
     CDS             1182..1439
                     /locus_tag="KPD58_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265897.1"
                     /translation="MIIIRSQDKTNLMQINQIKIDGSQVYAVFESKIDTVKIGDYENN
                     TRAIQVLDNIQNFIENGTKYDYITSNKVRYNVNKIFQMPAK"
     gene            complement(1549..2229)
                     /locus_tag="KPD58_002134"
     CDS             complement(1549..2229)
                     /locus_tag="KPD58_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBG0265898.1"
                     /translation="MIWTFYSFICVILPCLIYQIVVIKRKNLKVEKNVMIHLVWVYIF
                     LLYIYLALSKAGIGSIWDIGRYSGLFRIDEINLIPFDSVGILTYILNIIMFMPLGFLL
                     PLIWKNFRNIINVSLTGLGFSLAIELCQLFNLRATDIDDLMMDTLGAVLGYLIWVGVN
                     NLFNLIKKKDIFPSIYKNIIQDEIAITIDDDISEKITSLYKNEAIIYLILAVLGEFLL
                     FNWSIIPY"
     gene            2489..2788
                     /locus_tag="KPD58_002135"
     CDS             2489..2788
                     /locus_tag="KPD58_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265899.1"
                     /translation="MKWIKWYSITCICIFIVVAFYMFIFPNKIETIDTSSAYSFVEKK
                     VPNSAVYQGYKKNPVDGTTTIYYSYDNSTHIVRLSHPEDYSRKINWDKVSNIRFD"
     gene            2851..3921
                     /locus_tag="KPD58_002136"
     CDS             2851..3921
                     /locus_tag="KPD58_002136"
                     /inference="COORDINATES: protein
                     motif:HMM:NF015983.1,HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG0265900.1"
                     /translation="MNKNQIFKSNNLNFDMLSNGVAIYFCLTNRCNLFCKHCCVESSS
                     NKKDLLSTYDLKNIVDIISVLNTPHIIITGGEPLIREDFFEIIKYIKQKLPDTKLILS
                     TNGTLIDEHNVDFIITNFEKIDISIDGVDENTCSETRGKGVFNKVISSVNLLKDNGFS
                     NIKLSMVFGQKNSHLNKQFKDLNKKLGTEFVERYFIPTGRGLENKESYLIEDTVLPIT
                     IPYILDNELKMCKDKKNKKMGCCACNAGNSQIFINYDGDVYPCPSLIKDDYKMGNIFD
                     NEFVKVIKENKTRDLKSLIKLESIYPYNFEKCKDCDVNIFCWHCPAILDSVKDNELEL
                     DRWCSMMKHNLNTAVWGDEKVN"
     gene            3918..4934
                     /locus_tag="KPD58_002137"
     CDS             3918..4934
                     /locus_tag="KPD58_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG0265901.1"
                     /translation="MKFTIFVTEDCNLRCTYCYEGNDKKKENMSYETADKTIDFICNK
                     ELESNSDYTTIVLHGGEPFINFDLIKYIHRDLVDRLSDKKILFDITTNGTIINDEIIE
                     FIAKNVKNISISIDGKKNIHDINRIDANGVGTYEKVMENGKRLIDKGIYVRARMTYNS
                     NTVKYLYESVYDIAKQGFKNIAPISDFMDKNWDNSHIKILNEQISKIMNIKKDYPEID
                     ISILDKQFINKKKGDCFGGINSYAIDTRGDVYPCTVCVGDKDILLGNIYDGLDDKKIK
                     NLIKIYISKNEECDGCTRLDYCMSTRCKLINKKVTGKFQSAVPIYCGLERVYVETTKK
                     LCSL"
     gene            4990..5151
                     /locus_tag="KPD58_002138"
     CDS             4990..5151
                     /locus_tag="KPD58_002138"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265902.1"
                     /translation="MIHIKKIEEKIELQDVEVQADPCKWYYDKHGIISQIDCMVDCAG
                     STPKGTVAY"
     gene            5359..7905
                     /locus_tag="KPD58_002139"
     CDS             5359..7905
                     /locus_tag="KPD58_002139"
                     /inference="COORDINATES: protein motif:HMM:NF014717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0265903.1"
                     /translation="MINIKLAVSYLKRQKGKTLSLILSIGIAVMLVFSLSVINESQGQ
                     SDINHAYKNSGDYHAFYEDINSDIVKELNGEEDIKEFNDVLKFGEIISKDNGASLNLC
                     SYNKDFLDSTRYKLVKGREPRTSNELVIEQKVLDKMELDVKLNQTIDFQIVNEHINKN
                     NENSIYSKDKQFKIVGILSKSDEYYENLNKYKVRAFTYSNNGKDIVPYELITHMGTLK
                     LNTKDLDSQKVVNLGRKYKLTSNQFYPNTEVSMSMYMKNISKKTTYSIKQSLLPLLCA
                     TLVIYNMFNIIILDMTKQIGLLRAVGASKRNIRQVFFIQSIVVLSVGIVIGLLGGIVF
                     SYLGLMIVYDKSAELTINTNSIIESIIMSIIAVILASVVPIYKAGNLSIIDAIKKTDK
                     LNYIPRLLLLKSHKKVVGIINEIAFKNIFRNKVTSIIIIITISLCGVLFIGRLTSTKF
                     IYGKESDSANITSKSYGNFDIYLGYSPINANYTFSKYDNSLLNEIKAIEDVTDVEPNI
                     YLKGYLRNADLEEDYLDELKRTEINNNNESTVLIRGYNKELLNNIDKYIDKGENVYSY
                     TDRDYKNVLLVNNFYSRIKLSNNTQIIKSPKIGDLIDIKIPVYKDGEYKYINTKVRIA
                     GIMKNSYISEQDGESQSGGIQIIFNEKDLKELTGISDYNKVFVKIKKETDKKVIKEIN
                     NIIEKSGFSDIEGRYVRNHFGDHYNKSSYKLAMICVFGVLLISSINIIFIVRSNIIVR
                     LSEICTFRAIGMSKKNVKRMLIKENMIYGVLSMIVAGIISSFNYYKIVSETNKLNQQV
                     YGINNSIKFEIPIYEILIFGSISVFVCVIAVYFSNKMINKLSIVSGIKEN"
     gene            7947..9191
                     /locus_tag="KPD58_002140"
     CDS             7947..9191
                     /locus_tag="KPD58_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG0265904.1"
                     /translation="MNSSNKYFLDCICEKIINNNQVILSNKVNGRWVKIPIECYEVLK
                     YSVDNALQLGNLYDVFQENEDKKYYERVIDKLEHIGLLSDSILSEFRLERINKISFSP
                     TNRCNLSCDYCCVDSKIDNNDYLDTSQVRKVIDNIVKLNPRILTITGGEPLMRNDFLE
                     LLDYIKEKFKGKIILSTNATLIKEKQIDDIINGLYAIEISLDGYDELSCSEVRGKGVF
                     CKVLNVIRLIKLKKFENVGLSMVVGKNNEKDIEKFNKLNEDLGTTPIIRNFMNIGRGN
                     ENYSKYLNDKLDIDYICKDDYVDESSLCSNMCKAGVNQISIDHKGDVYPCPNLEYKDL
                     KIFNILEFNENILERILNREMDIFNIFDKLKPVNIESCKECKVNLFCTTCPSKIYTLK
                     NNKKMFDANCKKMKNILEPILW"
     gene            9239..10228
                     /locus_tag="KPD58_002141"
     CDS             9239..10228
                     /locus_tag="KPD58_002141"
                     /inference="COORDINATES: protein motif:HMM:NF012311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBG0265905.1"
                     /translation="MTKVRIAVLDTGLDVNDDDINGNYVYDSNFQLKDLGGKEINDIN
                     GHGTFVSKTILNISKNTQIYPINIFNDSGKTSSLNLLSTLSKLTMSDIQIINISAASI
                     NSMYEKELEKVCNILNSQGRFIICSKHNKYEDKESIPTIFESVIGVVGNENIYKNNQY
                     IYEPENKIQMYANSKDNFIKFKDKVTHFGKNSKACAVATGIVANILKNNPYIKFNELE
                     EILIQKSKSKEDLKIENLNTPSYKSNLIRLDIARKIINVINRNFSKIKIDFEFVSEYS
                     LFNNFTCIGNYNAYNFLTILNKEFNISIDYKEVYLYQLENLDMAVDLIYENLI"
     gene            10265..10939
                     /locus_tag="KPD58_002142"
     CDS             10265..10939
                     /locus_tag="KPD58_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0265906.1"
                     /translation="MKILKVKDLKKIYGKYENEVRALDGIDLDIEPHKFTAIVGASGS
                     GKSTLLHCVAGLDKPTLGNVYLDDVDLYTLNDDKLSEIRRQEFGFIFQSYNLIPVINV
                     YDNIVLPVLLDGKKEDKEYVMRLINKLGIEKQIKKYPNELSGGQQQRVAIARALANRP
                     SVIFADEPTGNLDSKTTNEVMNMLEWCVKEYKQTLVMITHNDNIAKTADRIITIKDGK
                     IDEKNY"
     gene            10923..12665
                     /locus_tag="KPD58_002143"
     CDS             10923..12665
                     /locus_tag="KPD58_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0265907.1"
                     /translation="MKKTINNNLFRILNIIVYKNKTKLIVSFIMVFIIAGVNIVIPQL
                     IRCIIDDGIGNKNIFLLINLILIYIFIRILSATFDVILSYLYTSIKNKVTINLKVKLL
                     NHISKLSGRYYSSKKTGNILSIMESDIFMIENFGAEIIFDIIVNVFTAIISLIFLINL
                     QFDLFLVVLFLQLILTFSQNKMTKLISKKISEIRSDSGSLSNLIQEYVSNIMDIVILN
                     SKKNFFNKYLKRERDLIKKDLNLNIIMSSNIAISQILTGLITILIYGIGGYKIINNKM
                     TMGELIAFQQYTGMLIGPCIAIVRANTRIAQSKVSINRVFSVIDEKIDIKVDNNGYRI
                     DNSEIKKIDFKNVSFRYGEDLNLVLNDINLQFEKGEVTGIVGGSGCGKSTITNLIYRL
                     WDINEGDILIDGVNIKDINLKSLRNNIDIVTQDQFVFDDTIKNNIVLNNKISDKELDE
                     ILEVTCLDDFIKNLDKGLDEVVGEKGVKISGGQKQRISIARALVSNKKVLILDEATSA
                     LDNISQKEILNNLKEYIQDKIVIIIAHRLSTIKSADNIYVLDKGRVIESGNDAELLHK
                     DGLYNKLVETADFA"
BASE COUNT         5401 a         1148 c         1956 g         4375 t
ORIGIN      
        1 ggaaattttg gataatcaaa atatatttta taaaatcaaa atacaattac ataaacttta
       61 ttaaaaattt gttgggaatt tctgactaac attaagaacg taagtttgtt gggggatggt
      121 atatgtggaa aagtcaaaca ctactgttac aaaaaaagaa aaaattgaag atataggaat
      181 gaaattagaa catttattga gtgaagatat tactttttat gaattattac ttgagatttt
      241 gtataaagaa aaagaagaga attaattctc ttctttaagg aatcttttta aataggcatt
      301 atcaatcatt ttttctatag cttctctatc ttctttttca agagaatata ttttttctaa
      361 gagctttttt gttttttcac ttactgataa gttctctatg attaatttat cttctttgct
      421 tatttttgta ttatcattta cgcctaataa ataatctgtt gatgtttcta atgcttttgc
      481 taatctaact attatttcac ttttaggttc tcttaaatta ttttcataac gtgacaagct
      541 agcctcagtt ataccgactt tagtagcaag ttctttttga tttatatcta attcttttcg
      601 tagatttgct attctatctc ctaaactttc catatgtaca tcactcctta ccgaaattat
      661 aagttatttt gataaaaagt aaaataaaat taccaaaaat ataaaaaaaa aaggttaaaa
      721 taataaaata tattgaaaat taccatttgt atatgtataa tatgaattat acaaatggta
      781 atttgatgag aggtggtatg ttgaaataag aaactgaatt aattaataaa tacatagaaa
      841 gttttgaaaa cctttaattg agatataaac aatatgtaca aaattttttg caagtaaaat
      901 taccaaatgt ataaaataaa tcagtagcat agataatttt atgctttaat acattagatt
      961 atttaattta gttataatgg tcaaaaatgc aatctacaaa taaaacaaag tattatccaa
     1021 aaagtattct ttaagaaaat tttttattag agatgtaatt gatataaata atcgtattaa
     1081 aaaataaaat actaagttta aaaatttaaa taatctataa atcaattata aaatattatt
     1141 tcttagaaat taaaatgaat taagtaaatg gaggaattga tatgataata atcagaagtc
     1201 aagataaaac taatttaatg cagataaacc aaattaaaat agatggtagt caagtttatg
     1261 cagtatttga aagtaaaatt gatactgtaa aaataggaga ttatgaaaat aatacaagag
     1321 ctatacaagt gttggacaat atacaaaact tcattgaaaa tggaacaaaa tatgattata
     1381 taacatcaaa taaagtcaga tacaatgtta ataaaatatt tcagatgcca gctaaatagt
     1441 gagtgtgcat tttaatttaa taactaaagc ccactttctt ttgtagtatg aaagagggct
     1501 ttacatattt gtaaatatcg aagtacactt gttttatcaa taaattattt aataaggtat
     1561 aatggaccaa ttaaacaata aaaattctcc taaaacagca agtattaaat atataatagc
     1621 ttcatttttg taaagagagg taattttttc gcttatgtca tcatcaattg taatagcaat
     1681 ttcatcttga ataatatttt tatatataga tgggaaaata tcttttttct ttataagatt
     1741 aaataaatta tttacaccta cccatatcaa atatcctaaa acagcaccta atgtgtccat
     1801 cattaaatca tcaatatctg tagctcttaa gttaaacaat tgacaaagtt caattgctaa
     1861 tgaaaaacct aatccagtta atgaaacatt gataatattt ctaaaatttt tccaaattaa
     1921 aggtagtaaa aatcctaaag gcataaacat aattatattt aaaatatatg ttaagatgcc
     1981 aactgagtca aaaggtatta gatttatttc atcaattcta aaaagcccac tataccttcc
     2041 tatatcccat atgctaccaa ttccagcttt actaagtgct agatagatat ataataaaaa
     2101 aatatatacc catactaaat gtatcattac attcttttca actttcaaat tttttcgttt
     2161 tataacgact atttggtata ttaagcatgg taatataacg caaataaatg aataaaatgt
     2221 ccatatcatt ttattttgtc ccctttcaaa tttatgtttt tagttttata agtactatct
     2281 ttatattaaa ctgtaagttt taaaaatctt ttaataaaat cttaaaaatt tattaattta
     2341 taaaaatata ttattaagat taaaaaatat atataatcaa agtttctaca attatatagt
     2401 tagggatata aaaattttat cgtatgtata aatattataa atatatggaa ataataatag
     2461 tatattttgg taaagtagga ggaattttat gaaatggatt aaatggtata gtattacatg
     2521 catatgtatt tttatagtag tagcttttta tatgtttata tttcctaata aaatagaaac
     2581 tattgatact tcatctgcat atagctttgt tgaaaagaaa gtaccaaata gtgctgtgta
     2641 tcaaggatac aaaaagaatc cagttgatgg tactactaca atttattaca gctatgataa
     2701 ttcaactcat atagttagat tatcacatcc agaagactat tcaaggaaga ttaattggga
     2761 taaagtttca aacatcagat ttgattaatc tttaatattt ataaaagtct atatatctaa
     2821 ggcaaatata ttattaatct tatagatatt atgaacaaaa atcaaatttt taaaagtaac
     2881 aatttaaact ttgatatgtt aagcaatggt gttgcaattt atttttgttt gacaaataga
     2941 tgcaatttat tttgtaaaca ttgttgtgtt gaatcttctt ctaataagaa agatttactt
     3001 agtacatatg atttaaaaaa tatcgtagat ataatatcag ttttaaatac tcctcatata
     3061 attataactg gtggagaacc attgataaga gaagattttt ttgaaataat taaatatata
     3121 aaacaaaaat tacctgatac caaattaata ttatccacaa atggaacttt aatagatgaa
     3181 cataatgttg attttattat aactaatttt gaaaaaatag atattagtat tgatggagta
     3241 gatgaaaata cttgtagtga aacacgagga aaaggtgtat ttaataaagt aataagctct
     3301 gttaatctgc taaaagacaa tggattcagt aatataaagc tgtctatggt atttggacaa
     3361 aaaaattctc atttaaataa acaatttaaa gatttaaata aaaagcttgg aacagaattt
     3421 gttgaaagat attttattcc aacaggaaga ggcctagaaa ataaagaaag ctatttaatt
     3481 gaagatacag ttcttccaat tacaattccg tatattttag ataatgaatt aaaaatgtgt
     3541 aaagataaaa aaaataaaaa aatgggatgt tgtgcatgta atgcaggaaa ctctcaaata
     3601 tttataaatt atgatggaga tgtatatcct tgtccttcac tgataaagga tgactataaa
     3661 atgggaaata tatttgacaa tgaatttgta aaagttataa aagaaaataa aacaagagat
     3721 ctaaagtctt tgattaaatt agaaagtatt tatccatata actttgaaaa atgtaaagat
     3781 tgtgatgtaa atattttttg ttggcattgc ccagcgatat tagattcagt taaggataat
     3841 gaattggaat tggatagatg gtgtagtatg atgaaacaca atttaaatac agctgtatgg
     3901 ggagatgaaa aagttaattg aaatttacta tatttgtaac tgaagactgc aatttgagat
     3961 gtacatattg ttatgaaggt aatgataaaa aaaaagaaaa tatgagttat gaaactgctg
     4021 ataaaacaat agattttatt tgtaataaag aattagaaag taatagtgat tatacaacaa
     4081 ttgttttaca tggaggagaa ccatttataa attttgattt gattaaatat atacataggg
     4141 atttagtaga tagattgagt gacaaaaaga tattatttga tataactaca aatggaacta
     4201 ttataaatga tgaaataata gagtttatag caaagaatgt aaaaaacata tcgataagta
     4261 ttgatggaaa aaagaatata catgatataa atagaataga tgctaacgga gtaggtactt
     4321 atgaaaaagt aatggaaaat ggaaaaagac ttatagataa aggtatatat gttagggcaa
     4381 gaatgacata taactcaaat actgttaagt atctttatga gagtgtatat gatatagcaa
     4441 aacaagggtt taaaaatatc gcaccaattt cagattttat ggataaaaat tgggataatt
     4501 ctcatattaa aatattaaat gaacaaatta gtaaaattat gaatattaaa aaggattatc
     4561 ctgagataga tatatctatt ttagataaac aatttataaa taagaaaaaa ggagattgtt
     4621 ttggagggat aaatagttat gcaatagata ctagaggaga tgtatatcca tgtacagtat
     4681 gtgtaggaga taaagatatt ttactaggaa atatatatga tggattagat gataaaaaaa
     4741 ttaagaacct tataaaaatt tatatatcta agaatgaaga gtgtgatgga tgcactagac
     4801 ttgattattg tatgtcaacc agatgtaaac ttatcaataa aaaggtaaca ggtaaattcc
     4861 aaagtgcagt accaatttat tgtggattag aaagagttta tgtggaaacg acaaaaaaac
     4921 tatgtagttt ataatctagt ttaactagtt ataataaata taaaacaaaa gtaaaggggg
     4981 atatgtatta tgatacatat aaaaaaaatc gaagaaaaaa tagaacttca agatgttgag
     5041 gtgcaagcag atccatgtaa atggtattat gataagcatg gaataatttc acaaatagac
     5101 tgtatggtgg attgtgcagg atcaactcca aaaggaacag tagcttatta aaaaatacta
     5161 atcagtctat taaatatgtt gaaagtacta gatgtagttt tttacaaatg aaatgtgatt
     5221 ttctcaactc tagtattttt aataattaaa tttaactagc acaacgagtt agttagtatt
     5281 taatgaatag tgctctataa cctactattt taggttatag agtttttctt gtaaatatga
     5341 ataaggagga ttctgattat gataaatatt aaacttgcgg tgtcatattt aaagagacaa
     5401 aaaggaaaaa ctttatcatt aatacttagt atagggattg ctgttatgct agttttttca
     5461 ttaagtgtta ttaatgaatc tcaaggacaa tcagatataa atcatgcata caaaaactcg
     5521 ggagattatc atgcttttta tgaagatata aatagtgata tagtaaaaga acttaatggt
     5581 gaagaagata taaaagaatt caatgatgta cttaaatttg gagaaattat aagtaaagat
     5641 aatggagcat ctctaaattt atgctcttac aataaagatt ttttagattc tactagatat
     5701 aagttagtta aaggtagaga gcctagaact tctaatgaat tagtaataga acaaaaagtt
     5761 ttagacaaaa tggaattaga tgtcaaatta aatcaaacta ttgattttca gattgttaat
     5821 gagcatataa ataaaaataa tgaaaattct atttatagta aagataagca atttaaaata
     5881 gttggtattt taagtaaatc tgatgaatac tatgaaaatt taaataaata taaggtaaga
     5941 gcttttacat attcaaacaa tggcaaagat attgttccat atgaattaat aactcatatg
     6001 ggtacattaa aattgaatac aaaagattta gattctcaaa aagtagtgaa tctagggaga
     6061 aaatataagt taacaagtaa tcaattttat ccaaatacag aagtaagtat gtctatgtat
     6121 atgaaaaata tttcaaaaaa aacaacttat tcaataaagc aaagcttact ccctttatta
     6181 tgtgcaacat tagtaatata caatatgttt aacataataa tactagatat gaccaagcaa
     6241 attggtttac ttagagctgt tggagctagc aagagaaata ttagacaggt attttttatt
     6301 cagagtattg tagttttatc tgtaggtata gtaattggtt tacttggagg tatagttttt
     6361 tcttacttag gtctcatgat agtatatgat aaaagtgcag aattaactat aaatacaaac
     6421 tctataattg aatctatcat catgtcaatt atagcagtta tattagcctc tgtagtacca
     6481 atatataaag ctggaaattt atctataatt gatgctataa aaaagacaga taagttaaat
     6541 tatattccta ggcttttgtt gttaaaatca cataaaaaag ttgttggaat tataaatgag
     6601 atagctttta aaaatatatt taggaataag gttacatcaa taataataat aattacgata
     6661 tcactatgtg gggttttatt tataggcaga ctaacttcta caaaattcat atatggaaaa
     6721 gagtcagatt cagctaatat aacaagtaaa tcatatggca attttgatat ttatttggga
     6781 tattcaccaa taaatgcaaa ttatacattt tctaaatatg ataattcttt attaaatgaa
     6841 ataaaagcta ttgaagatgt aacagatgtt gaacccaata tttatttaaa aggatatcta
     6901 agaaatgctg acttagaaga agattattta gatgagctaa aaagaactga aataaataat
     6961 aataatgagt ctacagtttt aattcgaggt tacaataaag agctattaaa taatatagat
     7021 aaatatatag ataaaggcga aaatgtttat agttatacag acagggatta taaaaatgtt
     7081 ttattggtaa ataattttta ttctagaatt aaattgtcaa ataatactca gattataaaa
     7141 tcacctaaaa taggagactt aatagatata aaaattcctg tatataaaga tggagagtat
     7201 aaatatataa atacaaaagt aagaatcgct ggaataatga aaaatagcta tatatcagag
     7261 caagatggag aatctcaatc aggtggaatt caaattatat ttaatgaaaa ggatttaaaa
     7321 gaattaacag ggataagtga ttataataaa gtttttgtga agataaaaaa agaaacagat
     7381 aaaaaagtaa taaaagaaat aaataatata atagaaaaaa gtggattttc agatatagag
     7441 ggaagatatg tacgtaatca ttttggagac cattataata aatcctcata taaattagca
     7501 atgatttgtg tatttggagt tttattaata tcatctataa acattatttt tatagttaga
     7561 tcaaatataa ttgtgaggtt gagtgagata tgtacattta gagcaatagg gatgtccaaa
     7621 aaaaatgtaa aaagaatgtt aataaaagaa aatatgatat atggggtatt aagtatgatt
     7681 gtagctggaa ttatatctag tttcaattat tataaaatag tttctgaaac taacaaactt
     7741 aatcaacagg tatatggtat aaataactct attaaatttg aaattccaat atatgaaatt
     7801 ctaatatttg gatctatatc tgtttttgtt tgtgtcattg cagtttattt ttctaataaa
     7861 atgataaata aacttagtat agtaagtggt ataaaagaaa attaatgaaa tttctaattt
     7921 aaatatatag gaaggaaata aaataaatga atagctcaaa caaatatttt ttagattgta
     7981 tatgtgaaaa aataataaat aataatcaag ttattcttag taataaagta aatggaagat
     8041 gggttaaaat tccaatagaa tgttatgaag ttctaaagta ttcagtagat aatgcattac
     8101 aacttggtaa tttgtatgat gtattccaag aaaatgaaga taaaaaatat tatgaacgag
     8161 ttatagataa gttagagcat ataggacttc taagtgattc tattctaagt gaatttagac
     8221 tagagagaat aaacaaaatt tcattttctc caactaatag atgtaatcta agttgtgatt
     8281 attgctgtgt agattctaaa atagataata acgattattt agatacatct caagtaagaa
     8341 aagtaataga taatatagtt aaattaaatc caaggatatt aaccattaca ggtggagagc
     8401 cattgatgag aaatgatttc ttagagctgt tggattatat aaaagaaaaa tttaaaggta
     8461 aaataattct gtctacaaat gcaactttaa ttaaagaaaa acagattgat gatataatta
     8521 atggtttata tgcaatagaa attagtttag atggttatga tgaattaagt tgtagtgaag
     8581 taagaggtaa aggagtattc tgtaaagtat taaatgtgat tagacttata aaactaaaaa
     8641 aattcgagaa cgtaggtttg tctatggtgg taggaaagaa taatgagaaa gacatagaga
     8701 aattcaataa attaaatgaa gatttaggta caacgcctat aataagaaat tttatgaata
     8761 ttggtagagg taatgagaac tatagcaaat atcttaatga taaacttgat attgattaca
     8821 tttgcaaaga tgattatgtg gatgaaagta gtttatgttc aaatatgtgt aaagcaggtg
     8881 ttaatcagat aagtatagat cataaaggag atgtatatcc gtgtccaaac ttagagtata
     8941 aggatttaaa gatattcaat atattagaat ttaatgaaaa catattagag aggattctta
     9001 atagagaaat ggatatattt aatatatttg ataaattaaa accagtaaat attgagagtt
     9061 gtaaagaatg taaagtaaat ttattttgta caacttgtcc atcgaaaata tatactttaa
     9121 aaaataacaa aaaaatgttt gatgcaaatt gtaaaaaaat gaaaaacatc ttggaaccta
     9181 tattatggta aggattagtt ttatattcaa ggtaatttat tttagaggtg attattaatt
     9241 gactaaagta agaatagcag tcttagacac aggattagat gtaaatgatg atgatattaa
     9301 tggaaattat gtttatgatt caaattttca attgaaagat ttggggggga aggaaataaa
     9361 tgacataaat ggacatggaa cttttgtatc gaaaaccata ttaaatataa gtaaaaatac
     9421 ccaaatttat cctattaata tatttaatga ttctggaaaa acaagttctt taaatcttct
     9481 tagtacactg agcaagttga ctatgtcgga tatacagata ataaacattt cagctgcatc
     9541 tattaatagt atgtatgaaa aagaattaga aaaggtttgt aatattttaa atagtcaagg
     9601 tagatttatt atttgttcaa aacataataa atatgaagat aaagaaagta ttcctactat
     9661 atttgaaagt gttattggtg tagtaggtaa tgaaaatata tataaaaata atcaatatat
     9721 atacgaacca gaaaataaga ttcaaatgta tgcaaattca aaagataatt ttattaaatt
     9781 taaagataaa gtaactcatt ttggtaaaaa tagtaaggct tgtgcagtgg caactggcat
     9841 tgttgcaaat atattaaaaa acaatccata tattaagttt aatgaattgg aagaaatact
     9901 tattcaaaaa agcaaaagta aagaagattt aaaaatagaa aacttgaaca ctccaagtta
     9961 taaatctaat ttgataagat tggatatagc aaggaaaata ataaatgtga taaatagaaa
    10021 cttttctaaa ataaaaatag actttgaatt tgtaagtgaa tattctttat ttaacaattt
    10081 tacatgtatt ggaaattata atgcatataa ttttttaact attttaaata aagagtttaa
    10141 tattagtatt gactacaaag aagtatactt atatcaatta gagaatttag atatggcagt
    10201 agatcttatt tacgaaaatt taatttaaaa atatatgttt tttaaatggg aatggagata
    10261 cgttatgaaa atacttaagg taaaagattt aaaaaaaata tatggaaaat atgagaatga
    10321 agttagagct cttgatggta ttgatttaga tatagagcca cataaattta cagctattgt
    10381 aggtgcaagt ggttctggta aaagtacact acttcactgt gttgcaggct tagacaagcc
    10441 aactttaggg aatgtgtact tagatgatgt agatttatat acattaaatg atgataagct
    10501 gtctgaaata agaagacagg aatttggctt catatttcaa agttacaatt taataccagt
    10561 tataaatgta tatgataata tagtacttcc tgtgttgctt gatgggaaaa aagaagataa
    10621 agaatatgtt atgagattaa ttaataagtt ggggattgaa aaacaaataa agaaatatcc
    10681 gaatgaacta tcaggaggtc aacaacaaag agttgcaata gctagagctt tagccaatag
    10741 accgtcagta atatttgcag atgaacctac tggcaactta gatagtaaga ctacaaatga
    10801 agttatgaat atgcttgaat ggtgtgttaa agagtacaaa caaacactag ttatgataac
    10861 tcataatgat aatattgcaa agacagctga tagaattata actataaaag atggaaaaat
    10921 agatgaaaaa aactattaat aataatttat ttagaatact aaatataata gtatataaga
    10981 ataagactaa gttgatagta tcatttataa tggtttttat aattgctggc gtaaatattg
    11041 taatacctca actaattaga tgtataatag atgatggaat aggaaataaa aatatttttc
    11101 tgcttataaa tttaatattg atatatattt ttataaggat attatcagct acatttgatg
    11161 ttatattatc atatttatat acttcaataa aaaataaagt tactataaat ctaaaagtaa
    11221 agttgcttaa ccatatatca aaactttctg gtagatatta ttcaagtaag aaaacaggta
    11281 atattttatc cattatggaa agtgatatat ttatgataga aaactttgga gcagagatta
    11341 tatttgatat tattgtaaat gtgtttacag ctataatatc gttaattttc ttaattaatt
    11401 tgcagtttga tttattttta gtagttttat ttttacagtt gatattaaca ttttctcaaa
    11461 ataaaatgac aaaacttata tctaagaaaa tttctgaaat aagaagtgat tcaggcagtt
    11521 tatcaaattt gattcaagag tacgtatcta atattatgga tattgttata ttaaattcaa
    11581 aaaagaattt ctttaataag tatttaaaga gagagagaga tttgataaaa aaagatttga
    11641 atttgaacat cattatgagt agtaatatag caatatctca aatactaaca ggattgataa
    11701 caatattaat atatggaatt gggggatata aaataataaa taataagatg acgatggggg
    11761 agttaatagc atttcaacaa tatacaggaa tgttaattgg accatgtata gctatagtaa
    11821 gggcgaatac tagaatagca caatcaaaag tgtctatcaa tagagtattt tcagtaatag
    11881 atgaaaagat agatattaaa gttgataata atggatatag aatagataac agtgaaatta
    11941 agaaaataga ttttaaaaat gtatctttta gatatggtga ggatttaaac cttgttttaa
    12001 atgatataaa tttacaattt gagaaagggg aagtaacagg aattgttggt ggaagtggat
    12061 gtggcaaatc aacaataaca aatctaatat accgactttg ggatataaat gaaggtgata
    12121 tattaataga tggtgttaat ataaaggata ttaatttaaa atcactaaga aataatattg
    12181 atatagtaac tcaagatcaa tttgtatttg atgacacaat aaaaaataat atagtattaa
    12241 ataataaaat aagtgataaa gaattagatg agatattaga agttacttgt ttagatgatt
    12301 ttataaaaaa tctagataag ggattggacg aagtagtagg agaaaaaggt gtcaaaatat
    12361 caggaggtca aaaacaacgt atatcaatag ctagagcttt agtaagtaat aaaaaggtac
    12421 ttattctgga cgaagcaact tcagctctag ataatatttc acaaaaagag atattgaaca
    12481 acttgaaaga gtacatacaa gataagatag ttattataat agctcataga ctttcaacaa
    12541 ttaagagtgc tgataatata tatgtattag ataaaggtag agtaatagaa tctggaaatg
    12601 atgctgaact tctacataaa gatggtttat ataataagtt agttgaaact gcagattttg
    12661 cataagcatg actttgtatg tacatatatg attattaggg tatttaatgg aatctataag
    12721 ttatataact aatttagtta tataaaagtt tgcaaatcaa tctcagataa tgttaaacaa
    12781 atgattagta tatccagtag aaaaattata atagtagtag aatatattac aaataaatca
    12841 aaacttcaca taattttcat atgaattatc aattaaattc
//
LOCUS       DAELLV010000099        12713 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.099, whole genome shotgun sequence.
ACCESSION   DAELLV010000099 DAELLV010000000
VERSION     DAELLV010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12713
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.099"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            267..1328
                     /locus_tag="KPD58_002144"
     CDS             267..1328
                     /locus_tag="KPD58_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HBG0265908.1"
                     /translation="MRDFLKNYKSSIILLACTIVGAIVGLVLGPKASILKPFGDLFIN
                     ALLMIVVPLVFLSVSSGVASIKNMERFGKIMKGTAIVFMGTTLVIAFLGITSALLFNP
                     SKGLDVSSVKNLMVAQDAETLESKGTLLDQVVSSITVKNFGDLFSSSNLLQLVIFAVL
                     IGTAIAMMGDRGNTIRKLLSEGTEAILKVVSIFMLYAPIGLGCYTATIIGELGSKIIQ
                     GYVRVFVIYSLTAAMYFLLFYSLYAFLSAGKQGFRQFWRNAIKPIATALATSSSSACI
                     PSNLEASKNIGVPDDISETVIPLGINIHKDGAVLAVIIKVAFLSGILGSGMANPATIG
                     QLFITVFVVGVFMAPVAGG"
     gene            1341..1538
                     /locus_tag="KPD58_002145"
     CDS             1341..1538
                     /locus_tag="KPD58_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HBG0265909.1"
                     /translation="MLVVSIFGFPVEAIPLLTVITTITDIPNTILNTTGNTVSSMLVS
                     RLVEGKDWLIDKTAITTKKIS"
     gene            complement(1702..2430)
                     /locus_tag="KPD58_002146"
     CDS             complement(1702..2430)
                     /locus_tag="KPD58_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG0265910.1"
                     /translation="MKTNYRLHMQQGSDYLKKFLEKHIDLLKKIPLFEGIKSDELEEM
                     LECLGVVDKTYPKDSIIFSVGSEVTSIGIMLKGSAHIIKEDIEGNRNIVAELLPGDLF
                     GEVFACTRLHKSNVTVTTTSSCEVLFIKFKSVTGICSSACVFHNRLVENMLQLIAEKN
                     ILLHNKIELLSRKTTREKLLMYFSKQIEQTGSHQFTIPFSRNEMADFLCVDRSSMSRE
                     LGKMRDEGLLSFNKNKFEIFYFQK"
     gene            2571..3296
                     /locus_tag="KPD58_002147"
     CDS             2571..3296
                     /locus_tag="KPD58_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG0265911.1"
                     /translation="MMRKIIKIDEEKCNGCGLCVEACHEEAIGMVNGKAKLLRDDYCD
                     GLGDCLPTCPTNAISFEYREAAEYDEAAVKANMEAKKAQKKTLACGCPGSQSKSINRE
                     VSNSTSISNDIVEIKGSQLNQWPVQIKLVPTNASYLKNASLLIAADCTAYAYGNFHNK
                     FMKNKVTLIGCPKLDEVDYAEKLTEILKENDIKNIVVTRMEVPCCGGIVNAVKTALQN
                     SGKMIPWQIVTISVDGKIVDGEI"
     gene            3356..4948
                     /gene="hcp"
                     /locus_tag="KPD58_002148"
                     /gene_synonym="priS"
     CDS             3356..4948
                     /gene="hcp"
                     /locus_tag="KPD58_002148"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HBG0265912.1"
                     /translation="MEKMFCFQCEQTAGGKGCTINGVCGKKGGTANLQDELTGVLIGL
                     SRATIGNKNRPTSETDKIMIEGLFTTITNVSFDDEAIKRQIEKTEVEKSKLVPRCSDC
                     STTCNRNDNYDMKDLWNDNEDIRSLKSLILFGLRGMAAYAYHAMVLGYTDKDVNEFFY
                     EGLIAVGGDLSIDELLGLVMKTGEINLKCMELLDRANTETYGTPEPTQVSMKIEKGPF
                     IVVTGHDLYDLHKLLEQTKDKGINIYTHGELLPAHAYPKLKEYKHLKGNFGTAWQNQQ
                     KEFDNIPAPILFTTNCLMPVKDSYRDRVFTTEVVAYPGMVHIDEDKDFSSVIEKALEL
                     GGYREDVHMTGINGGDTLTTGFARGTVSSVQDKVVDAVKSGAIRHFLLVGGCDGAKPG
                     RNYYTEFVKQAPKDTVILTLACGKYRFNDLDLGDIGGLPRLMDMGQCNDAYSAIKVAV
                     SLANAFECGVNDLPLSMVLSWYEQKAVCILLTLLHLGIKNIYIGPSLPAFLSKNVLDI
                     LVDKFALTPISTPEEDMKKILG"
     gene            5319..6224
                     /locus_tag="KPD58_002149"
     CDS             5319..6224
                     /locus_tag="KPD58_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBG0265913.1"
                     /translation="MKNSNFNILAFDGGGLKGALSISILERIVKEYPNLLNNINMFGG
                     TSTGSLIALGLAYGVSPKEIKELYSIENSKYIFNKSYAEILRPKYENKNLKEVLLSIF
                     PEELELKDLNKLVMIPSFYIGNEENAWKPVFYNNMPNSFTKTSKVVDVAMASSAAPVF
                     FPTYNRHVDGGIIATDPSLACIIHAIDSGFKLKNTRLFSIGTGYVYNSIKADTTEWGA
                     IDWIINKEPDLPIISITLEGNSQMSQLFSQKLLGDNYYRLNPKMEKDVAMDDCDALEY
                     LMSLGENCDIKDAFGWIKKKSFYRN"
     gene            6348..7301
                     /gene="msrA"
                     /locus_tag="KPD58_002150"
     CDS             6348..7301
                     /gene="msrA"
                     /locus_tag="KPD58_002150"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBG0265914.1"
                     /translation="MIKLATFAGGCFWCMVKPFDKYDGVIKVVSGYTGGHVENPTYQE
                     VCSGTTGHLEAIQITYDDDIISYDELLNIFWKQINPTDDGGQFGDRGTQYKTAIFYHD
                     EEQKNIALESREHLENSKIFDSKIVTDIIPATKFYEAEENHQDYYKKRPLNYNMYYKG
                     SGRYNFVKSHWDGNNINREELKKTLTPIQFEVTQNDMTEVPFENEYYDNNKEGIYVDI
                     VSGEVLFSSKDKFDSGCGWPSFSKPVIDKSIIERSDFSQGMYRVEVRSAKGNSHLGHV
                     FDDGPKELGGLRYCINSASLKFIPRENMEALGYGEYLKLFE"
     gene            7454..8584
                     /locus_tag="KPD58_002151"
     CDS             7454..8584
                     /locus_tag="KPD58_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0265915.1"
                     /translation="MNLKDFNGKRLKSARIFRAKTIEQLSKETKINKKDLKAFEENKY
                     VPNIENTLKLSNILNFPKEYFYKNENINIVVEDSHFNPQSKLPRVEEISYREKIIIIH
                     KIYSFMENYIKFPKENLPNRKVMSDININDIESLAYKTREFWDLNSTPIVNMVSLLES
                     KGIIISGMNVDRKGATIFTQKQRISKESKYLISLGNDKKSASIRNYTLACELGYIISS
                     ELRIPSKQFSEDEYACAFLLPKESFLKDLTHPEDLDYYVELKKKWIVPISAMIFRAYN
                     LEKINYKKYNYLMNEMDKKGWLIEEPLDKMKGSSPTYLKRAVELLIENKIMSVNSIVS
                     SLEEFRINLYPEDLELLMGLKKGLLSQEVNKKSKVIKFDGKK"
     gene            complement(8701..9669)
                     /locus_tag="KPD58_002152"
     CDS             complement(8701..9669)
                     /locus_tag="KPD58_002152"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBG0265916.1"
                     /translation="MAIKPRKISIVGSGHVGSHCGFSLITQGVCDELFMIDIDESKSK
                     AQALDLADAVSYLPHKVHIEKGTFSDCKDSDIVVISVADSSEGPLRRQNTTRLDLLRP
                     TIGMIKSIVKPIVDSGFDGIFVVISNPVDVVTNYIWEKSGFPKNKVIGTGTALDSTRL
                     RRILSEETGIAQQSIQAYSMGEHGDSQMVPWSHVSIGGKPILDMIKDNPSTYSNLDLP
                     SIVEKNKKTGISIINGKDCTEFGIGTALVEIVKAILHNEKKVLPVSTLLEGQYNERNV
                     FAGVPCVIGKDGIEEIIEINMTEYEQNEFNKSCSVLREYIALSKTL"
     gene            10003..10191
                     /locus_tag="KPD58_002153"
     CDS             10003..10191
                     /locus_tag="KPD58_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265917.1"
                     /translation="MNYHTKEELVEALRVVSSSIINCGKGQKKFSEETSHHTRFKNII
                     EVMYISKSLIMDEISKRD"
     gene            10451..11650
                     /locus_tag="KPD58_002154"
     CDS             10451..11650
                     /locus_tag="KPD58_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein AlaX-L"
                     /protein_id="HBG0265918.1"
                     /translation="MEKLYYTNQYIRDFTAEIIEIKEVENKYHVLLDKTAFFPGGGGQ
                     FGDLGKIENDDVINVYEDGKKIYHVLDKKPIKIHKVKCSIDWDRREDGMHQHFGQHVL
                     SGCFFKTFNANTTGFHLGNEYSTVDIEGNLEEDKIREIELFANEIIRQNIQVETLLPT
                     KKELKKIWLRRDLPNTDEEIRVIKIGDLDTNACCGVHPKSTGELRMIKIKKWEKHKSS
                     TRIEFLAGKRAVDEVLKRDIYLTKICRYLSCSEEDAINGIVNLNSKVEEALIKKRRLE
                     EVVSTYQVKEMIEKANKVGNISVIKKIYDDEDLKYVNKIANKIAEYENSIALLAVKFN
                     DKINLVFACSKNLKDINMGVLLKDAISLIDGKGGGSKVLAQGGGKNNGNLEATLDYAF
                     MKIEKTL"
     gene            11702..12205
                     /locus_tag="KPD58_002155"
     CDS             11702..12205
                     /locus_tag="KPD58_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0265919.1"
                     /translation="MEQQKDYIELYENEELIEIREARMDDLDTIAKFNYNLAKETEGK
                     ELDMDVLTKGVKALLLDERKGKYHVYTVFDKVVAQIMYTYEWSDWRNGNFLWIQSVYV
                     DKEYRRKGIFNYLFNYIKNICDKDENIVGMRLYVEKENINAKATYESLNMYECDYNMY
                     EYEVVHS"
     gene            12312..12629
                     /locus_tag="KPD58_002156"
     CDS             12312..12629
                     /locus_tag="KPD58_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265920.1"
                     /translation="MGVLKGMFYVFLFLDLVSIFCFFFNKGKIASNKIVFNAIGVLTF
                     VLCFMLFSYYPNNNLIGKFIASLFFIFGVAGVMLKEKNFLYARLLLTVVIVFSTLRLF
                     VIQ"
BASE COUNT         4934 a         1348 c         2196 g         4235 t
ORIGIN      
        1 ttttttatat tgtttgtttt attaaaatta atctagaaaa aaagttttaa aagcagaatt
       61 ttattaatat ttatatatag atatgaacta ttaataaaac gttaaaaata caaaaaaact
      121 atagaaattg acatatattc cagtatttgc taaagtagct gttggggaaa attacaaaaa
      181 ttattaatgc gcattagata atttgcttta atttttttag catttggggt aatttataat
      241 ataaagcatt agaaaggaaa atataaatga gggacttttt aaaaaattat aagtcatcaa
      301 ttatactttt agcatgtact atagtagggg cgatagtagg attagtactt ggtccaaaag
      361 cttcaatact aaaaccattt ggagatttat tcataaatgc tctattaatg atagtagtac
      421 cattagtttt tcttagtgtt tcttcaggtg ttgctagtat aaaaaatatg gaacgatttg
      481 gaaaaataat gaagggtaca gctatagtat ttatgggaac aacacttgta attgcatttt
      541 taggtattac atctgcatta ttatttaatc cgtctaaagg attagatgta tcaagtgtaa
      601 aaaatttaat ggtggctcaa gatgcagaaa ctttagaatc aaaaggaacg ttattagacc
      661 aagtagtatc aagtattaca gttaaaaatt ttggagattt attttccagt agtaatttac
      721 tacaattagt tatatttgca gttttaattg gaacagcaat agctatgatg ggagataggg
      781 gaaatacaat aagaaagctt ttatcagaag gaacggaagc aatattaaaa gtagtaagta
      841 tattcatgct ttatgctcct atagggctag gctgttatac agctacaata attggggaac
      901 ttggaagtaa aataattcaa ggatatgtaa gggtgtttgt aatttattca ctaacagcag
      961 ccatgtattt cttattattt tacagtctgt atgctttttt atctgcagga aaacaagggt
     1021 ttagacaatt ctggagaaat gctataaaac caatagcaac ggcactagct acttcttcaa
     1081 gttcagcatg tataccatca aatttagaag ctagtaaaaa tataggagtt cctgatgata
     1141 tatcagaaac agttatacct cttggaataa atatacataa agatggggca gttttagcag
     1201 taataataaa agtggcattt ttatcaggta ttttaggtag tggaatggca aatccagcaa
     1261 cgataggtca attatttata actgtatttg ttgttggagt atttatggct cctgtagcag
     1321 gtggctgact tattggtgag atgctcgtgg ttagcatctt cggttttcca gtggaggcaa
     1381 ttccactgtt aacagttata actactataa ctgatattcc taatactata ttaaatacaa
     1441 ctggaaatac agtatcatca atgttagttt caagattagt agaaggaaaa gattggctta
     1501 tagataaaac agcaattact acaaagaaaa taagctaatt gtacacttaa ttaataaagt
     1561 taataggtat cttaatttta ataaattaga tacctattaa ctttttaagt aaaaatataa
     1621 atttaagatt caaatatgat atttataatt tgaatcaact aatatagtat atattgaaat
     1681 aaatatgttt aaatatctat tttatttttg aaaataaaag atttcaaatt tatttttgtt
     1741 aaaagataac aagccttcat ctcgcatctt gccaagctct cttgacatag aacttctgtc
     1801 aacacataaa aaatcagcca tttcatttct tgaaaaggga attgtaaact ggtggctacc
     1861 tgtttgttct atttgttttg aaaaatacat aagcaatttc tcgcgagttg ttttacgaga
     1921 aagtaattct attttattgt gaagtaaaat gtttttttca gctattaatt gtagcatatt
     1981 ttcaacaagc ctattatgaa atacacatgc agatgaacaa ataccagtta cacttttaaa
     2041 ctttataaat aaaacttcgc aagaagatgt tgtggttact gtaacattac ttttatgtag
     2101 tctagtacat gcgaatacct ctccaaataa atctccaggc aagagttcag ctacaatatt
     2161 cctattccct tcgatatctt cctttattat atgagcactt cctttaagca taattccaat
     2221 agaagtaact tcacttccaa cagagaatat tatactatct tttgggtaag tcttatctac
     2281 aacacctaaa cattccaaca tttcttcaag ttcatcactt ttaattcctt caaatagagg
     2341 aattttttta agtaggtcta tatgcttttc taaaaatttt ttcaaataat cacttccttg
     2401 ttgcatatgc aaccgataat ttgttttcat tgtactatac taagtttata aaagcaaggg
     2461 gatataaaaa tcctactaca agaaatttga aataaagaaa tataggtatt aaaaataaat
     2521 atataagaaa tcaatacttt aaattaaaaa aattttatga ggtgatataa atgatgagaa
     2581 aaataattaa aattgatgag gaaaagtgta atggttgtgg actatgtgtt gaagcttgtc
     2641 atgaagaagc tataggaatg gtaaatggaa aagcaaaact tttaagagat gactattgtg
     2701 atgggctggg agattgtctg ccaacttgtc caacaaatgc gatttcattt gagtatagag
     2761 aagcagcaga atatgatgaa gctgcagtaa aagcaaatat ggaagcaaag aaagcacaaa
     2821 agaaaacttt agcatgtgga tgtccaggtt ctcagtctaa atcaataaat agagaggttt
     2881 caaatagtac gtctataagc aatgatatag tagagataaa aggttctcaa cttaatcaat
     2941 ggccagtaca aataaaatta gtaccaacta atgcatcata tttaaagaat gcaagtcttt
     3001 taatagcagc agactgtaca gcgtatgcat atggaaattt tcataataaa tttatgaaaa
     3061 ataaggttac attaatcgga tgccctaaac ttgatgaagt tgactatgct gaaaaactta
     3121 cagagatttt aaaagaaaat gatattaaaa atatagtagt aacacgtatg gaagttcctt
     3181 gctgtggtgg tatagtaaat gctgttaaga cagctctaca aaatagtgga aaaatgatac
     3241 catggcaaat agttactata tctgtagatg gaaaaattgt agatggagaa atttaattaa
     3301 gtgaattata gaaaaatata ataaatttta ataaataaaa agggaggaat aaaaaatgga
     3361 aaaaatgttt tgttttcaat gtgaacaaac agcaggaggt aaaggttgta ctataaatgg
     3421 tgtctgtggt aaaaaggggg gtacagctaa cttacaagac gaacttacag gagtgttgat
     3481 tggtctttca agagcaacta ttggaaataa gaatagacca acaagtgaaa cagataaaat
     3541 tatgatagag gggctattca ctactataac gaatgtaagt tttgatgatg aagctataaa
     3601 aagacaaatt gaaaaaactg aggtagaaaa aagtaaactt gtacctagat gttcagattg
     3661 ttcaacaact tgtaatagaa atgataacta tgatatgaag gatttatgga atgataatga
     3721 ggacatacgt tcattaaaat cattaatctt atttggatta agaggtatgg ctgcctatgc
     3781 atatcatgca atggtacttg gttatacaga caaagacgta aatgaatttt tctatgaagg
     3841 attaatagca gttggaggag acttgtctat tgacgaactt ttaggtctag ttatgaaaac
     3901 tggagaaata aacttaaaat gtatggaatt gcttgataga gcaaatactg aaacttatgg
     3961 aacaccagaa ccgactcagg tatctatgaa aatcgagaaa gggccattta ttgtagttac
     4021 aggacatgac ctttatgatt tacacaaatt attagaacaa acgaaagata agggtataaa
     4081 tatatatact catggtgagt tattaccagc acatgcatat ccaaaactaa aagaatataa
     4141 acatctaaaa ggcaattttg gtacagcatg gcaaaaccag caaaaagaat ttgataatat
     4201 tcctgctcca atattattta caacaaactg tttaatgcct gttaaagata gttatagaga
     4261 tagagtattt actacagaag tagttgctta tccaggtatg gttcatattg atgaagataa
     4321 agatttttct agtgtaatag aaaaagctct tgaacttgga ggatatagag aagatgttca
     4381 tatgactggt attaatggag gagatacatt aactactggt tttgcaagag gtacagtttc
     4441 atctgtacaa gataaagtag tagatgctgt aaagagtgga gctataagac actttttatt
     4501 ggttggaggt tgtgatggtg caaaacctgg aagaaattat tacactgaat ttgtaaaaca
     4561 agctcctaaa gacactgtta tattgacact tgcatgtgga aaatatagat ttaatgattt
     4621 agaccttggg gatataggtg gcttacctcg tttaatggat atgggacaat gtaatgatgc
     4681 ttatagtgca attaaagtgg cagtttcatt ggcaaatgct tttgaatgtg gagtaaatga
     4741 tttgcctctt agcatggtac tgtcatggta tgagcagaaa gctgtttgta tacttcttac
     4801 actacttcat ttaggtatca aaaatatcta tatagggcct tctttaccag cattcttatc
     4861 taagaatgta ttagatatat tggttgataa atttgcattg acacctatat ctactcctga
     4921 agaagatatg aagaagattt taggataaat aaatatatct atacttaaag atatttatag
     4981 ttaaaatatt tttaagtatg ccacaattaa gataaggggt taaacttaaa atttgattaa
     5041 aaaatcaatt ttgagatgac cctttattta ttttttgtaa taattgaaaa ataaacctgc
     5101 tttcagtata ttcttttaag aatttttgta ttaaatttat aatacttaga tatataatat
     5161 cttatatatt ttattttaag tattaaaggt ttgaatgtaa cttaaatatt aaaagaggaa
     5221 cattgtaata tgtaacttgc ataaaatata tatactatag tgaaattatt ttgattaaaa
     5281 tttaaaatat aaggtgtttt atgtgggggt atatgtatat gaaaaattct aattttaata
     5341 tattggcatt tgatggtggt ggattaaaag gggcattgag tatatccata cttgagagga
     5401 tagtaaaaga gtatccaaat ctattaaaca atataaatat gtttggaggt acatctacag
     5461 ggagtctaat tgcactaggg cttgcgtatg gtgtaagccc taaagagata aaagaacttt
     5521 actcaattga aaattcaaag tatattttta ataaaagtta tgcagaaata ctcagaccaa
     5581 agtatgaaaa caaaaactta aaagaagttt tattaagtat atttccagag gaactagaat
     5641 taaaagattt aaataaatta gtaatgatac caagttttta tataggaaat gaggaaaatg
     5701 catggaaacc agtgttttat aacaatatgc caaattcttt tacaaagact agcaaagttg
     5761 tagatgtagc aatggcaagt agtgctgctc cggtattttt tccaacatat aatcgtcatg
     5821 ttgatggagg aataatagca actgacccga gtttagcgtg tataattcat gccattgaca
     5881 gtggatttaa acttaagaat acaagacttt tttcaatagg gactggatat gtatataaca
     5941 gtataaaggc agatactact gaatggggag ctattgactg gataattaat aaagaacccg
     6001 atttaccaat tatatctatt accttagaag gaaattctca aatgagtcag ttattttctc
     6061 aaaaacttct tggagataat tactatagat taaatcctaa gatggaaaaa gatgtagcta
     6121 tggatgattg tgatgcatta gaatatctaa tgtctttagg agaaaattgt gatataaaag
     6181 atgcttttgg atggataaag aaaaagagct tttatagaaa ttagattcaa tttgcaattg
     6241 tatttttaca catgatttca ttgagtaggt aaaaataata aaatgcagat tattaagtta
     6301 cttcttaggg aataaataaa gtataattta tttaaggagt gatgaaaatg ataaaacttg
     6361 ctacttttgc aggtggatgt ttttggtgta tggtaaaacc ttttgataag tatgatggag
     6421 ttattaaagt agtttctgga tatacaggtg gtcatgttga aaaccctaca tatcaagagg
     6481 tatgctcagg aactactggt catttagaag caattcaaat aacatatgat gatgatataa
     6541 taagttatga tgaacttcta aatatcttct ggaaacagat taatcctaca gatgatggtg
     6601 gacaatttgg tgatagggga actcaatata agactgctat attttatcat gatgaagaac
     6661 aaaaaaatat agctttagag tcaagagaac atctggaaaa tagtaaaata tttgatagta
     6721 aaatagtgac tgatataatt ccagcaacta aattctacga agcagaagaa aatcatcagg
     6781 attattataa gaaaagacca ttaaactaca atatgtatta taaagggtcc ggaagatata
     6841 attttgtaaa aagtcattgg gatggaaaca atataaatag ggaagagtta aaaaagactt
     6901 taactcctat acaatttgaa gttactcaaa atgatatgac agaagtacct tttgaaaatg
     6961 aatattatga caataacaaa gagggaattt atgttgatat tgtaagtgga gaagtgttat
     7021 tttcttcaaa agataaattt gattctggat gtgggtggcc aagtttttca aagccagtta
     7081 tagataaatc tatcattgag agaagtgatt tctcacaagg tatgtacaga gttgaagtaa
     7141 gaagtgctaa aggaaattct catttaggtc atgtatttga tgatggtcca aaagaacttg
     7201 gaggtttaag atattgtata aattctgctt ctcttaaatt tataccaaga gaaaatatgg
     7261 aagctttagg atatggagaa tatttaaaac tttttgagta attaggaata tttattataa
     7321 acttatctaa aaggtgaaaa tctaaaaaaa tattttcacc ttttgttttt ttgtcaatag
     7381 tatgataaat tattaattgt aatcttattt ataccaatag attaagaatt taattttgaa
     7441 agagagaaaa caaatgaatt taaaagactt taatggaaag agattaaaat cagctagaat
     7501 ttttagagca aagacgatag aacagttgtc taaagaaact aaaataaata aaaaagattt
     7561 aaaagctttt gaggaaaata agtatgtacc aaatatagaa aatacattaa aattatctaa
     7621 tatattaaat tttccaaaag agtattttta taaaaatgaa aatataaata tcgttgtaga
     7681 ggatagccac tttaaccctc aatctaaact acctagagta gaagagattt cttatagaga
     7741 aaaaataatt ataatacata aaatatattc atttatggag aattatatta agtttccaaa
     7801 agaaaacttg ccaaatagaa aagtaatgtc agatattaat ataaatgata tagaatctct
     7861 tgcatataag actagagagt tttgggattt aaatagtact ccaatagtaa atatggtaag
     7921 tttgttagag tctaaaggga ttataatatc aggtatgaat gtagatagaa aaggggcaac
     7981 tatctttact caaaaacaga gaattagtaa agaatctaaa tatctaatat ctctgggtaa
     8041 tgacaaaaag tcagcatcaa ttagaaatta tactttggca tgtgaacttg gatatataat
     8101 atcaagtgaa ttaagaatac caagtaagca attttcagaa gatgagtatg cctgtgcatt
     8161 tttattgcca aaagagagtt ttttaaaaga tttgactcat ccagaagatt tagattatta
     8221 tgtagaacta aagaaaaaat ggattgtacc tatatctgct atgattttta gagcatataa
     8281 tctagaaaag ataaattaca aaaaatacaa ttatcttatg aatgaaatgg ataaaaaagg
     8341 ctggttgata gaagaaccac tagacaaaat gaaaggttca agcccaacgt atttaaaaag
     8401 agcagttgaa ttattgatag aaaataaaat aatgagtgta aatagtatag tatcaagttt
     8461 agaggaattt cgaataaatc tttatccaga agacttagaa ttattgatgg gattgaaaaa
     8521 aggattgtta tctcaagagg taaataaaaa aagtaaagtt ataaaatttg atggtaaaaa
     8581 ataggtttat attttagtat taaaattaaa agcaggtaca aatgatttaa acattaactt
     8641 agttaaaagt tttttcaata agtcatttac ctgcttttag tgtaagaaag aatattgaat
     8701 ctaaagtgtt tttgaaagtg caatatattc ccttaaaaca gaacaagatt tattaaattc
     8761 gttttgctca tattcagtca tattgatttc aataatttct tcaataccat cttttcctat
     8821 aacacatgga acacctgcaa atacatttct ttcgttatat tgaccttcta gtaatgtaga
     8881 aactggaaga acttttttct cattgtgtag tatagctttt acaatttcta ctaaagcagt
     8941 acctatacca aattctgtac aatctttacc atttattata cttattcctg tttttttatt
     9001 tttttcaact attgatggta agtctaaatt gctataagta cttgggttgt cttttatcat
     9061 gtctaagatt ggtttaccac caattgaaac atgagaccaa ggaaccattt gagaatctcc
     9121 atgttcaccc atagaataag cttgaattga ttgttgagcg attccagttt cttcagataa
     9181 aatccttcta agtcttgttg aatctaaagc tgttccagtt ccaatgactt tgtttttagg
     9241 aaagccagat ttttcccaaa tataatttgt aactacatca acaggattag atatgactac
     9301 aaatatacca tcaaagccag aatcaactat gggttttact atagatttta tcattccaat
     9361 agttggtctt agtaaatcta atctagttgt gttttgtcta cgtaaaggcc cttctgaaga
     9421 gtctgcaaca cttatcacta ctatgtcaga atctttacag tcagaaaaag ttcctttttc
     9481 tatatgtact ttatgtggca gatatgaaac tgcatcagct aaatctaaag cttgagcttt
     9541 tgatttagat tcatctatat caatcataaa gagttcatca caaactcctt gagttattaa
     9601 actaaatcca caatgagaac caacatgccc tgaacctaca atagatattt ttcttggctt
     9661 tattgccatt ttaaatcact ccccctaaaa taacgataca ttgaaaatta tagatgttat
     9721 taaaagctgt ttttggatat tttaatattc tattatttca gaaaatttat ttaatatgat
     9781 tttatactaa aatttttaaa aaagcaatgt taatatactc tctattagtg aatattaaag
     9841 tattatatgt atattaataa ttattaaaat atgtaatgtc actgatagtt agaatattta
     9901 agtgctaaaa gatgtgtata aataataatg atgtcagttt ataatgatat taaattgaga
     9961 tatactgttt ctctaaatta aaaaataaag gagtaaatgg ttatgaatta tcatacaaaa
    10021 gaagaattgg tggaagcact gagagtagta tcttcaagta ttattaattg tggaaaagga
    10081 cagaagaaat tttctgagga gacatctcat catacacgtt ttaagaatat tatagaagta
    10141 atgtatattt caaaatcatt aataatggat gaaattagta aaagagattg aagcttattt
    10201 tgcaatttaa catgttttaa tatacatttt attatataag aaaataagta aacatacatg
    10261 ttataaaata ttgaaataat atacaattat aaatttgtta acattaatat ctatatgtta
    10321 aaatatagta gaagatgtta gtaaataata ggatgagttc tattttaata caattaaatt
    10381 aagcttagtt tgaaatttgt atctaaaaag taaagtaatc tagaattaag aaaatggaga
    10441 tagaattata atggaaaaat tatattatac aaatcagtat ataagagatt ttacagcaga
    10501 aataattgaa ataaaagaag tagaaaataa gtatcatgtt ttattagata agacagcttt
    10561 ttttcctggt ggaggaggtc agtttggaga cctgggaaaa atagagaatg atgatgtaat
    10621 aaatgtgtat gaagatggaa aaaaaatata ccatgtatta gataaaaaac ctataaagat
    10681 acataaggta aagtgtagta ttgattggga tagaagagaa gatggaatgc atcaacattt
    10741 tggacaacat gtattgtctg gatgtttttt taaaactttt aatgcaaata cgacaggttt
    10801 tcatttggga aatgagtata gtacagtaga tatagaaggt aatttagaag aggataaaat
    10861 tagagagata gagttgtttg ccaatgaaat tattcgtcaa aatatacaag tagagacatt
    10921 acttccaacg aaaaaagaac taaaaaagat ttggttaaga cgtgatttgc caaatactga
    10981 tgaagagata agagtaataa aaataggtga tttagatacg aatgcatgtt gtggagtgca
    11041 tccaaaatca actggtgaat tgagaatgat aaaaataaag aaatgggaaa aacataagtc
    11101 aagtacaaga atagagtttc tagcaggtaa aagggcagtg gatgaagtat tgaagaggga
    11161 tatatatttg actaaaatat gtagatattt aagctgtagt gaggaagatg ctataaatgg
    11221 tattgtaaat ctaaatagta aagtggaaga ggctttaatt aaaaaaagaa ggcttgaaga
    11281 agtagtatct acatatcagg tcaaagaaat gattgaaaaa gcaaataagg ttggcaatat
    11341 ttcggtcatt aaaaaaattt atgatgatga agatttgaaa tatgtaaata agatagcaaa
    11401 taaaattgca gaatatgaaa atagtattgc attattagct gttaaattta atgataaaat
    11461 aaatttagtt tttgcatgtt ctaaaaattt aaaagatatt aatatgggag ttttattaaa
    11521 ggatgcaata agtcttatag atggcaaggg tggtggaagt aaagtattag ctcaaggagg
    11581 aggtaagaat aatggaaatt tagaggctac acttgattat gcatttatga agatagaaaa
    11641 aactctataa atatattata taaaaaacct tagtagataa aatagatatg gaggaataag
    11701 tatggagcaa caaaaggact acatagagtt atatgaaaat gaagaactaa tagagataag
    11761 agaagcacgt atggatgatt tggatacaat agcaaaattt aattataatt tggctaaaga
    11821 aactgaagga aaagaacttg atatggatgt tttaactaaa ggtgtaaaag cattgttatt
    11881 agatgagaga aaaggaaaat atcatgtata tacagtattt gataaagttg ttgcacagat
    11941 tatgtataca tatgagtgga gtgattggag aaatggaaat ttcctttgga ttcagagtgt
    12001 atatgtagat aaggaatata gaagaaaagg tatctttaat tatttattta attatataaa
    12061 aaatatatgt gataaagatg aaaatatagt tggaatgaga ttatatgtag aaaaagaaaa
    12121 cataaatgca aaagcaacat atgagtcttt aaatatgtat gagtgtgatt ataatatgta
    12181 tgaatacgaa gtagtacatt cgtaatataa atttgtaata ctgttatact tatgttataa
    12241 agtattgatt atattgacga ttatatgaaa taaactggat attataatta gaatttttag
    12301 ggggtataaa aatgggcgtt ttaaaaggaa tgttttatgt atttttattt ttagatttag
    12361 tgtcaatatt ctgtttcttt ttcaataaag gaaaaatagc atctaataaa attgtgttta
    12421 atgcaatagg agtacttaca tttgtacttt gttttatgct ttttagttat tatcccaata
    12481 ataatcttat tggaaaattt attgcttctc tattttttat atttggagtt gcaggagtta
    12541 tgttgaaaga aaaaaatttt ttatatgcta gacttctttt aactgtagtt atagtttttt
    12601 ctacattaag attgttcgtt attcaataaa atgtatttgt atgaaaattt attaaagtct
    12661 acacatttaa gtgtaggctt ttttgatttt ttgtttgaaa attaaggaaa ttt
//
LOCUS       DAELLV010000100        12667 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.100, whole genome shotgun sequence.
ACCESSION   DAELLV010000100 DAELLV010000000
VERSION     DAELLV010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12667
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.100"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            122..835
                     /gene="ispD"
                     /locus_tag="KPD58_002157"
     CDS             122..835
                     /gene="ispD"
                     /locus_tag="KPD58_002157"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HBG0265921.1"
                     /translation="MYSVIIVAAGSGRRMNLDINKQFIKLREKEIIAHTIQVFYENIN
                     IDEIVVCIKKEEEDFFKENIINKYNFKNIKIAYGGKERQDSIYNGLKKLDKNCDIVLI
                     HDGARPFVDHRIINESIKVAKEKKAVVVGVPVSDTIKIVSDGTVKETPERNLLWAAQT
                     PQTFEYNLIIDAYEQAYKNNYYGTDDSMLVENIGQSVTMVMGSYENIKITSPEDLNIA
                     EQILNMEKRDEVNSRRRII"
     gene            832..1317
                     /locus_tag="KPD58_002158"
     CDS             832..1317
                     /locus_tag="KPD58_002158"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HBG0265922.1"
                     /translation="MRIGLGYDVHKLTEDRKLIIGGVEIPHDKGLLGHSDADVLIHAI
                     MDSILGALALGDIGKHFPDTDEEYKGADSMKLLEHVYNLITSKGYKIGNIDSTIIAQS
                     PKMAPYIESMRSNISKVLNTDIDNINIKATTEEGLGFTGAKQGIASQSICLLLLTSQN
                     N"
     misc_binding    1412..1620
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1684..3399
                     /locus_tag="KPD58_002159"
     CDS             1684..3399
                     /locus_tag="KPD58_002159"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBG0265923.1"
                     /translation="MKMSKMFMPTLKEIPADAEITSHQLMVRSGMIKKMTSGVYNQLP
                     MGLRVFKKIEQIIREELNKKDCQEILCAALLPSELWKESGRWTAMGEEMFRLKDRTER
                     EYCLGPTHEEAFTDIIRQEITSYKQLPLNLYQIQVKYRDERRPRFGVMRTKTFTMKDA
                     YSFDVDDKGLDKSYQDMFDAYVSIFNRCGLENSPVQADSGAIGGSTSAEFMVKSEVGE
                     DEVVFCSGCDYAANVERAESCNLASQKEEMKELEEVHTPGAATIKELEEFLKTSPDKF
                     AKTLVYEADGKTVVVVVRGDREVNEIKVSNAIGSVIEFALATDDVVRKVTNAEVGFAG
                     PIGINADYVFIDKEIVEQRNIVVGANKTEYHIKNANYGRDFEGIVGDFRNVQEGDKCI
                     VCGKPLEIARGVEVGHIFKLGTKYSESMNANFIDKDGKSKPIVMGCYGIGVERTAAAI
                     IEQHNDEKGIIWPLSVAPYHVVIVPANMKNEEQISIAENIYNDLQAMGVEVLLDDRDE
                     RIGVKFNDSELIGIPMRITVGKNINEGKVEFKLRHKEDKEIIDIEEINEKVKAEFIRN
                     NVRLG"
     gene            3521..4966
                     /locus_tag="KPD58_002160"
     CDS             3521..4966
                     /locus_tag="KPD58_002160"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBG0265924.1"
                     /translation="MAKNEKQFVEEITKMEDDFPQWYTDVITKTDLVDYAPVKGFMVV
                     KPYGYALWEKMQEFMDKKFKETGHKNCYFPLLIPESLLNKEAEHVEGFAPEVAWVTHG
                     GNKKLEERLCVRPTSETIICTMYAKWLKSYRELPYLYNQWCSVVRWEKSTRPFLRTSE
                     FLWQEGHTLHETAEEAQEETIQQLEVYKALCEELLAMPVVAGQKSESEKFAGGERTYT
                     IEAMMHDGKALQSGTSHFLGQHFTKAFDITFADREGNLANPYHTSWGASTRLIGGLIM
                     THSDNRGLVLPPRVAPIQVVIVPIAAKKGNVMETVDKIYADLKAKGVAVEVDDRDNYT
                     TGWKFNEWEMKGVPVRVEIGPKDIENNQAMVFRRDTLEKDSMPLEGLADAICDLFDVI
                     HNDMFEKARKHREDNTSIVENMDEFRKALEEKPGFIKTMWCGDAECEAKIKEETGATI
                     RCLPFEQENLGHKCVYCGKEADSMVVMAKAY"
     gene            5405..6886
                     /locus_tag="KPD58_002161"
     CDS             5405..6886
                     /locus_tag="KPD58_002161"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBG0265925.1"
                     /translation="MNVRVRFAPSPTGFVHIGSLRTALYNYLFAKKMGGEYILRVEDT
                     DQSRLVEGAIENMLNAMKWAGVNHSEGVILDDSGKVVQKGEYGPYIQSQRLDIYQEYI
                     KQLLDSGKAYYCFCTKERLDEVRDAQRAAGETAKYDGHCKNLSKEEVEANIKAGIPYV
                     IRLRLPENHTIKFTDLVRGDMEFNTNDLDDQVLMKTDGFPTYHFAVVVDDYLMKITHV
                     IRGEEWVSSTPKHVYLYEAFGWEAPVFVHLPNILNKEKKKLSKRQGDVAVEDFKKKGY
                     LPEGLVNYVALVGWSPEDNQELFTMEELEKAFSVERVSKSGGVFDTEKLNWVNQHYIK
                     DGDDAYLTDLAIPFLIEDGFITEEEATNKYDFLKSMISVLKEKLQYVKEVTEHASIFF
                     GDKIEVETEEGREFLRLEHIPTLIDALREKIEKTEVLNAEFVQAMLKEIQKEYKIKGK
                     NLFMGSRIILTGQMHGPDLPKVMEVLGKETCLNRIAYVKNNIL"
     gene            7107..7262
                     /locus_tag="KPD58_002162"
     CDS             7107..7262
                     /locus_tag="KPD58_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265926.1"
                     /translation="MYSNIDDVKKELKELCLEYVTILEKLKDEKMITEETFEKCSSQK
                     KIFLEEQ"
     gene            7410..8807
                     /locus_tag="KPD58_002163"
     CDS             7410..8807
                     /locus_tag="KPD58_002163"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBG0265927.1"
                     /translation="MKVYNTLTRTKEEFVPLEEGKVKMYVCGPTVYNYIHIGNARPFI
                     IFDTLRRYLEYRGYDVTYVQNFTDVDDKIINRSHEEGISPEEVAAKYIKEYFVDCDGL
                     GIKRATVHPQVTDNIQQIIEFIKELEDKGYAYAVNGDVYFDTNKFEGYGKLSGQKQED
                     LEAGARIEVNDQKRHPMDFVLWKAKKEGEPGWDSPWGEGRPGWHIECSVMSKRYLGET
                     IDIHAGGQDLTFPHHENEIAQSEARSGKTFSKYWMHNGYININDEKMSKSKGNFFTVR
                     DISKLYDLEIVRFFMLSAHYRNPVNFSDEMLNQAKAGLERLYNTKEKLEFTLSNLVES
                     PLTEKEVELVKELDDFRQKFIDAMDDDVNTADAVSVIFELAKLINSNVDENSSLEFAK
                     KCLDEFNELTGVLNIVNKKKDTVLDKDIEELIQKRTDAKKNKEFQLADDIRQQLLDMG
                     IVLEDTRQGVKWKRI"
     gene            8792..9205
                     /locus_tag="KPD58_002164"
     CDS             8792..9205
                     /locus_tag="KPD58_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HBG0265928.1"
                     /translation="MEKDIKMEKTELVTMSPLVLAYLGDTVYETYIREYLIRQNTQRK
                     VNDLHKLAIKYVKAKAQATIIHEIEIELTEEESKIYKRGRNQKSNTSPKNADIIDYKH
                     ATGFEALVGYLYLNNEIERLQYIINKGIKIIERDM"
     gene            9311..10069
                     /locus_tag="KPD58_002165"
     CDS             9311..10069
                     /locus_tag="KPD58_002165"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HBG0265929.1"
                     /translation="MKVKLISHTPEPEKVIAMAAKLCYSPVGTDEIEKDLTDESIEKF
                     LNMLLSIGHGSILEHASFTFSIEGISRACSHQIVRHRIASFSQQSQRYVKLEQFEYII
                     PPEIEKIEKAKELFIDSMKKDQENYDKLVEILFENHYNDLIKNGKNEKTAKRQAEKKA
                     IEDARYVFPNACETKMVFTINARSLFNFFEHRCCERAQWEIRNLAVEMLREVKKVAPI
                     LFKKTGPSCVNGSCPEGTMTCGDIVQVREKFKAL"
     gene            10082..10819
                     /gene="rlmB"
                     /locus_tag="KPD58_002166"
     CDS             10082..10819
                     /gene="rlmB"
                     /locus_tag="KPD58_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBG0265930.1"
                     /translation="MASIEGRNPVIEAIKSDREIDKILIANSAKEGSIKKIIGMAKDK
                     NIIIQYVDKHKLDEVSTSHSHQGVIAYASEYKYYELDELIDLAKNKDEDPFFIILDEI
                     TDPHNLGSIIRTADAVGAHGVIIPKRRSVHITPVVAKASAGAVEYMPVCKVTNIVNTI
                     KRLKEEGLWIAAADMDGETFYKQNLTGPLGVVIGSEGFGISRLVKQNCDFIVKMPMIG
                     NVTSLNASVAGGILLYEIFRQRLDKSK"
     gene            10822..11349
                     /locus_tag="KPD58_002167"
     CDS             10822..11349
                     /locus_tag="KPD58_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HBG0265931.1"
                     /translation="MKKNINHYLIIDGYNIINAWDNLKELAKEDLEDSREKLIDDIIE
                     FSEFMGYKTIIVFDAYNVKNSREKVEKRKHITIVYTREHQTADSYIEKFITSLSKYDD
                     VKVATNDYAEQQMILGKGATRMSARELKLELDRSKNKMKEKNIGLRKKIQRNWLEERL
                     DKETLSKLENIRRKR"
     gene            11414..12061
                     /gene="sigH"
                     /locus_tag="KPD58_002168"
     CDS             11414..12061
                     /gene="sigH"
                     /locus_tag="KPD58_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HBG0265932.1"
                     /translation="MLVAKEKSYELVDNCQQDEYNIVLRASEGDKIALEYIITKYRNF
                     VKAKAKSYFLIGADKEDIIQEGMIGLYKAVRDFDGSKTNSFKCFAEICITRQIITAIK
                     TATRQKHIPLNSYVSLNKPIYDEESDRTLLDIIATSIVTDPEELIISKEELKNIESKM
                     NELLSDLELEVLELYLNGKSYQFIADKLKRDVKSIDNALQRVKRKLEKHLENRND"
     gene            12135..>12667
                     /locus_tag="KPD58_002169"
     CDS             12135..>12667
                     /locus_tag="KPD58_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBG0265933.1"
                     /translation="MAKAKYERTKPHVNIGTIGHVDHGKTTLTAAITKTLYDRYQLGE
                     AVDFANIDKAPEERERGITISTAHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVCSATDGPMPQTREHILLSRQVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLT
                     EYDFPGDDTPIVRGSAL"
BASE COUNT         5268 a         1307 c         2486 g         3606 t
ORIGIN      
        1 gcaaatagac attaactatt tatacaactg aaaatttaat atataattaa tcagatggtt
       61 aaaataaaaa tattaaaagt tttaataaat gatggttaaa aataaaatac gaggtgatac
      121 catgtatagt gttataattg ttgcagcggg aagcggaagg agaatgaact tagatataaa
      181 caaacagttc ataaaattaa gagaaaaaga aattattgct catacaattc aagtttttta
      241 tgaaaatata aatatagatg aaatagtggt atgtataaaa aaagaagaag aagatttttt
      301 taaagaaaat ataataaata agtataactt taaaaatata aaaatagcat atggcggaaa
      361 agaaagacag gactcaatat ataatggttt aaaaaaatta gataaaaatt gtgatatagt
      421 acttatccat gatggagcaa gaccatttgt cgaccataga ataatcaacg aatctataaa
      481 ggtagctaaa gaaaaaaagg ctgtggttgt aggtgttcca gtaagtgata ctataaagat
      541 agtgtcagat ggaacagtaa aagaaactcc agaaagaaat ttgctgtggg cagcacaaac
      601 ccctcaaacc tttgaatata atctcataat agatgcttat gaacaagcat ataagaacaa
      661 ttattatggt actgatgatt caatgttggt agaaaatata ggtcaaagtg tcactatggt
      721 aatgggttct tatgaaaaca taaaaataac tagtccagaa gatttaaata tagcagaaca
      781 aatattaaat atggaaaaaa gagatgaagt taacagtaga aggagaatta tatgagaata
      841 ggattaggat atgatgtaca caaattaaca gaggatagaa aacttataat cggtggtgta
      901 gagataccac acgataaagg tttgttgggt cattctgatg ctgatgtatt gatacatgca
      961 ataatggatt ctattttagg tgctctagca ttaggagata tagggaagca tttcccagat
     1021 acagatgaag aatataaggg agcagatagt atgaaattat tagaacatgt gtataatcta
     1081 ataacaagta aaggatataa aattggtaat atagacagta ctataattgc tcaaagccct
     1141 aagatggctc catatattga gagtatgaga agtaatatat caaaagtttt aaacacagac
     1201 attgataata taaacataaa ggcaacaaca gaagaaggtc ttggttttac aggagcaaaa
     1261 cagggaatag cttcacaaag tatttgttta ttattgttga caagtcagaa taattaatat
     1321 attattagta ataagtttaa tggatatcaa gtaataggcc tatatagaat atctataatg
     1381 tatgaattta agtgaaatta atataaatat aaaattagct atgataaaga aatagtaaaa
     1441 tgatgaaatc ttcacagaga gaaaacggtg gtgagagttt tcaagaggaa gtctttgaac
     1501 ctgtctttta gcttaaaggt ataaaaatct ttacggtagg aaccgttata ttcccaaaag
     1561 agagctattc atatagctaa ctagagtggt accgcggaaa ttaaaccttt cgtctctata
     1621 cttgtataga gatgagaggt tttttatatt ttcaggaagt taatattcag gaggaatcta
     1681 ttaatgaaaa tgtcaaaaat gtttatgccg acattaaaag aaatcccagc agatgctgag
     1741 attacaagcc atcaattaat ggttaggtca ggcatgataa aaaaaatgac ttcaggtgtt
     1801 tataaccaat taccaatggg gttaagagtg ttcaaaaaaa ttgaacaaat aattagagaa
     1861 gaattaaata aaaaagattg tcaagaaata ctttgtgcag ccttacttcc ttctgaatta
     1921 tggaaagagt ctggaaggtg gactgctatg ggtgaagaaa tgtttagact aaaagacaga
     1981 acagaaagag agtactgtct tgggccaaca catgaagaag cattcactga tataataaga
     2041 caagaaatta cttcatataa acaattgcca ctgaacttgt accaaataca agtaaaatat
     2101 agagacgaga gaaggccaag atttggtgtt atgagaacta agacttttac aatgaaggat
     2161 gcctatagtt ttgatgtaga tgataaaggt ttagataagt catatcaaga tatgtttgat
     2221 gcatatgtaa gtatatttaa tagatgtgga ttggagaata gccctgtaca ggcagattca
     2281 ggagctatag gaggttctac atcagccgaa tttatggtta agtcagaagt tggtgaagat
     2341 gaagttgtat tttgtagtgg atgtgattat gcagccaatg tagaaagagc agaatcttgt
     2401 aatctagcat cacaaaaaga agaaatgaaa gaattagaag aggttcatac accaggagca
     2461 gctacaataa aagagttaga agaattttta aaaacttctc cagataagtt tgctaaaact
     2521 ttagtatatg aggcagatgg aaaaacagtt gtagtcgttg taagaggaga tagagaagtt
     2581 aatgaaatca aagtatctaa tgccataggt tctgttatag aatttgctct tgctacagat
     2641 gatgtagtga gaaaagtaac taatgcagaa gtaggatttg caggtccaat aggtataaat
     2701 gctgactatg tatttataga taaagaaatt gttgaacaaa gaaacatagt tgttggggct
     2761 aataaaactg agtaccatat aaaaaatgct aactatggaa gagattttga aggaatagtt
     2821 ggagacttta gaaatgttca agaaggcgac aagtgtatag tatgtggtaa gccacttgaa
     2881 atagctagag gtgttgaagt aggacatata tttaaactag gaactaagta ctcagaatct
     2941 atgaatgcaa acttcataga caaagatgga aaatcaaagc ctatagtaat gggatgttat
     3001 ggaataggtg ttgaaagaac agcagcagct ataattgaac aacataatga tgagaaaggt
     3061 ataatatggc ctttgtcagt agctccatac catgttgtga tagttccagc aaatatgaaa
     3121 aatgaagaac aaatatcaat agctgaaaat atatataatg atttgcaagc tatgggtgta
     3181 gaagttttat tagatgacag agatgaaaga ataggtgtca aattcaatga ttcagaatta
     3241 ataggaatac ctatgaggat tacagtaggt aaaaatatta atgaaggtaa agtagaattt
     3301 aaacttagac ataaagaaga taaagagata attgacatag aagaaataaa tgaaaaagta
     3361 aaggcagagt ttatcaggaa taatgttagg ttaggataat aacttaaata agatattgta
     3421 tagataatca aagttgtact ttatagacac cattttatct atatataagg atataaaatg
     3481 gtgttttttt ataaaacaaa taaatgagga ggtaattaaa atggcaaaaa atgaaaaaca
     3541 atttgttgaa gaaataacaa agatggaaga tgattttcca caatggtaca cagatgtaat
     3601 aactaagact gacttagttg attatgcacc agtaaaaggt tttatggttg taaagccata
     3661 tggatatgca ttatgggaaa agatgcaaga atttatggat aaaaaattta aagaaactgg
     3721 acataaaaac tgttattttc cattattaat accagagagt ttattaaaca aagaagcaga
     3781 acacgttgaa ggttttgctc cagaagttgc atgggtaaca catggaggaa ataaaaaatt
     3841 agaagaaaga ttatgtgtaa gacctacttc agagactata atatgtacaa tgtatgcgaa
     3901 atggttaaaa tcatatagag aattaccata tctttataat caatggtgct cagttgttag
     3961 atgggaaaaa tcaactagac ctttcttaag aacatctgag tttttatggc aagaaggtca
     4021 tacattacat gaaacagcag aagaagcaca agaagaaaca atacaacaat tagaagtata
     4081 taaagcgcta tgtgaagaat tacttgcaat gccagttgta gctggtcaaa aaagtgaaag
     4141 tgagaaattt gcaggtggtg aaagaacata tactatagaa gctatgatgc atgatggtaa
     4201 agcattacaa tcaggtacaa gtcacttcct aggtcaacac tttactaagg cttttgatat
     4261 tacttttgca gatagggaag gaaatttagc taatccatac catacatctt ggggagcatc
     4321 tacaagactt ataggtggac ttataatgac tcatagtgat aacagaggtt tagtattacc
     4381 accaagagta gctccaattc aagttgttat agttcctata gctgctaaaa aaggaaatgt
     4441 aatggagact gttgataaaa tatatgctga tttaaaagct aagggagtag ctgtagaagt
     4501 tgatgataga gataactata caactggatg gaaatttaat gagtgggaaa tgaaaggtgt
     4561 tccagtaaga gttgaaattg gaccaaaaga tatagaaaat aatcaagcta tggtatttag
     4621 aagagatact ttagaaaagg attctatgcc tttagaagga ttagctgatg ctatatgtga
     4681 tttatttgat gttatacaca atgatatgtt tgaaaaagca agaaaacata gagaagacaa
     4741 tacatctata gttgaaaata tggatgagtt tagaaaagct ttagaagaaa agccaggatt
     4801 cataaagact atgtggtgtg gagatgcaga atgtgaagct aagattaagg aagaaacagg
     4861 agcaactata agatgcttac cttttgaaca agaaaactta ggtcacaagt gtgtgtactg
     4921 tggaaaagaa gcagatagta tggtagtaat ggcaaaagct tactaaaata catttaaata
     4981 aaaactgggg tagaaagtat ttaatacttt atcccagttt ttttgttttt tatgagtaat
     5041 aaaatgtatt tgtaactatg tcataagata agtcatagat acttatatta aaaagaatat
     5101 aatagagaac tatttttaat aattacatat taatgaaagc aaaatatttt gttatataga
     5161 ttttcaataa agcaaacaat aaatataaat gcatattaat aaggagtgct tttaattttt
     5221 agaaaataaa tgacttaaaa gaaagattta tgaaattaaa actaggattt tctgatataa
     5281 taaacatgtg tatttttaaa taaaaaggat attattatta gatttgatta aaaataaact
     5341 tgcaaaacaa tgtactagtt taatattata gttatattga ataaaatatc aggaggaagt
     5401 aaaaatgaat gtaagagtaa ggtttgcacc aagtccaaca ggatttgtac atataggtag
     5461 tttgagaact gctttatata actatttatt tgcaaaaaaa atgggcggag aatatatatt
     5521 aagagtggaa gatacagacc aaagtagatt agtagaaggt gctatagaga atatgcttaa
     5581 tgctatgaaa tgggctgggg tcaaccatag tgaaggtgtt atattagatg atagtggaaa
     5641 agtagtacaa aaaggagaat atggtccata tattcaatct caaagattag atatatacca
     5701 agaatatata aagcaacttt tagatagtgg aaaagcttat tactgtttct gtacaaaaga
     5761 aagactggat gaagtaagag atgctcaaag agcagcagga gaaacagcta aatatgatgg
     5821 tcattgtaag aatctttcta aagaagaagt agaagcgaat ataaaagcag gaataccata
     5881 tgttataaga ttaagattgc cagagaatca tacaattaag tttacagact tagttagagg
     5941 agatatggaa tttaatacta atgacttaga tgaccaagta ttaatgaaaa cagatggatt
     6001 tccaacttat cattttgcag tagttgttga tgactatttg atgaaaatta cacatgttat
     6061 aagaggagaa gaatgggtat catctactcc aaaacatgtt tatttatatg aagcttttgg
     6121 atgggaagca cctgtatttg tgcatttacc aaatatactt aataaagaaa agaaaaaatt
     6181 aagtaaaaga caaggtgatg tagctgttga agactttaag aaaaaaggat atttaccaga
     6241 gggattagta aactatgtag cattagttgg ttggtctcct gaagataatc aagaattgtt
     6301 tacaatggag gaattagaaa aagccttctc tgtagaaaga gtatctaaaa gtggtggagt
     6361 atttgatact gaaaaattaa attgggttaa tcagcactat ataaaagatg gtgatgatgc
     6421 ttatctaaca gacttagcaa taccattttt aatagaagat ggatttataa cagaagaaga
     6481 agctactaat aaatatgatt tcttaaagag tatgatatca gtacttaaag agaaattaca
     6541 atatgtaaaa gaagttactg aacatgcaag tatatttttt ggagataaaa tagaggtaga
     6601 aactgaagag ggaagagagt tcttaagatt agaacatata cctactttaa tagatgcttt
     6661 gagagaaaaa atagaaaaaa cagaggtatt aaatgctgag tttgttcaag ctatgcttaa
     6721 agaaatacaa aaagagtaca aaataaaagg gaaaaattta tttatgggct caagaataat
     6781 tttgacaggg caaatgcatg gtccagattt accaaaagta atggaggttt taggaaaaga
     6841 aacttgctta aatagaatag cttatgtaaa aaataatata ttatagtcat aaaagtccgc
     6901 taatttagaa gcggactttt tttgtgttag tatttggtga aaatatctgc ttaagaaaat
     6961 gaaggtattt aaggtaataa atttaatata taaaaagcaa agcaaaaata taaataaaat
     7021 atgaataata aactaataaa atattaagca tagaagaagg gaatctatta ttttagtttg
     7081 atttaattat taagggggta attactatgt atagtaatat tgatgatgtt aaaaaggaat
     7141 taaaggagct atgcttagaa tatgttacaa ttttagaaaa attaaaagat gaaaaaatga
     7201 ttacagaaga aacttttgaa aagtgctctt ctcaaaaaaa aatattttta gaagaacaat
     7261 agaggatggt aatagtatta ttattattat gtagtttgaa agatagtacg aaaaatgata
     7321 gtaatactat tatttattgc aattttcaac tataatataa tatataatgt taagaagata
     7381 atttataaaa atacagtgag gtgatgagtg tgaaagttta taatacattg actagaacaa
     7441 aagaagaatt tgttccatta gaagaaggaa aggtaaaaat gtatgtgtgt ggtccaacag
     7501 tttataacta tatacacata ggtaatgcaa gaccatttat aatatttgat actctaagaa
     7561 ggtatttaga gtatagagga tatgatgtaa cttatgtaca aaactttaca gatgttgatg
     7621 ataagattat aaatagaagt catgaggagg gaatatctcc tgaagaagtt gcggctaagt
     7681 atataaaaga atattttgta gactgtgatg ggctaggaat aaaaagagct actgttcatc
     7741 ctcaggttac agataatata caacagatta tagagtttat aaaagagttg gaagataagg
     7801 gatatgcata tgcagtaaat ggtgatgtat attttgatac aaataagttt gaaggttatg
     7861 gaaaactttc aggtcaaaaa caagaagatt tagaggcagg ggctagaatt gaggttaatg
     7921 accaaaaaag acatcctatg gactttgttc tttggaaggc aaaaaaagaa ggtgagccag
     7981 gatgggatag cccttgggga gaaggtagac caggatggca tatagaatgt tctgttatgt
     8041 ctaagagata tttaggtgaa acaattgata tacatgcagg tgggcaagat ttaacattcc
     8101 cacatcatga aaatgaaata gctcaaagtg aagctagaag tggaaaaaca ttctcaaaat
     8161 actggatgca taatggatat ataaatataa atgatgaaaa gatgagtaaa tcaaaaggta
     8221 atttctttac agtaagagat atatcaaaat tatatgattt agagatagta agatttttca
     8281 tgttatcagc acattataga aatccagtaa actttagtga tgagatgtta aatcaagcta
     8341 aagcaggtct tgaaagacta tataatacta aagaaaaatt agaatttacg ctaagtaatt
     8401 tagtagaatc tcctttaaca gaaaaagaag tagagcttgt taaagaatta gatgacttta
     8461 gacaaaaatt catagatgct atggatgatg atgttaatac agcagacgca gtaagtgtaa
     8521 tatttgaatt agctaagtta ataaattcta atgtagatga aaattcttct ttggagtttg
     8581 ctaaaaaatg cttagatgaa tttaatgaac ttacaggagt attaaatatt gtaaataaga
     8641 aaaaggatac tgtattagat aaagacattg aagaattgat acaaaaaaga actgatgcaa
     8701 agaaaaataa agagtttcaa ttagctgatg atattagaca acaattacta gatatgggaa
     8761 ttgtattaga agacacaaga caaggtgtta agtggaaaag gatataaaaa tggaaaaaac
     8821 tgaattagtt actatgtcac cattagtttt agcatactta ggtgatacag tgtatgagac
     8881 atatataaga gaatatttaa ttagacagaa tacacaaaga aaagttaatg atttgcataa
     8941 actagctata aagtatgtaa aagctaaagc tcaggcaact ataattcatg aaattgaaat
     9001 tgagttaact gaagaagaga gtaaaattta taaaagggga agaaatcaaa agtcaaatac
     9061 ttctcctaag aatgcagata ttatagacta caaacatgca actgggtttg aggcattagt
     9121 tggatatttg tatttaaata atgaaataga gagacttcaa tatattatca ataaaggaat
     9181 caaaattatt gaaagagata tgtaaatatt aaagagtgtt gtcttaataa taagtaataa
     9241 ggcaacactt tttttataac taatgtataa taaatatata gaaattagta atgagaaagg
     9301 ataaaaagtt atgaaagtaa aattaatatc acatacacct gaaccagaaa aagtaatagc
     9361 tatggcagct aagctatgtt attcaccagt tgggacagac gaaatagaaa aagacttaac
     9421 agatgaaagt atagagaaat ttttaaatat gttattaagt ataggtcatg ggtcaatatt
     9481 agaacatgct tcatttacat tttcaataga aggtatttca agggcttgtt cacatcaaat
     9541 tgttagacat cgtatagcta gtttttcaca acaaagtcaa agatatgtta aattagaaca
     9601 atttgagtac ataattcctc cagaaataga aaaaatagaa aaggcaaaag agttatttat
     9661 agactccatg aaaaaagacc aagagaatta tgataagtta gttgaaatat tgtttgaaaa
     9721 tcattataat gacctaataa aaaacggtaa aaatgagaaa acagctaaaa gacaagctga
     9781 aaagaaagca atagaagatg ctagatacgt atttccaaat gcatgtgaaa caaaaatggt
     9841 atttactata aatgctagaa gtttgtttaa tttctttgaa catagatgct gtgaaagagc
     9901 acagtgggaa ataagaaatt tagctgtgga gatgttaaga gaggttaaaa aggttgctcc
     9961 tatattattt aaaaagactg gtccaagttg tgtaaatgga agttgtccag aaggaactat
    10021 gacttgtgga gatatagttc aagttagaga aaaatttaag gccttgtagg aggtgaataa
    10081 tttggcgagt atagaaggaa gaaatccggt aatagaagca ataaaaagcg acagagaaat
    10141 agataaaata ttaattgcaa attcagctaa agaaggttca attaagaaaa taataggaat
    10201 ggctaaagat aagaatataa ttattcagta tgttgataaa cataagttag atgaagtaag
    10261 tacaagtcat tcacatcaag gtgtaatagc ttatgcaagt gagtataagt attatgaatt
    10321 agatgagcta atagatttag caaagaataa agatgaagac ccatttttca ttatattaga
    10381 tgagataaca gacccacata atttgggttc aataataagg acagcagatg cagtaggagc
    10441 acatggagtt atcattccaa aaagaaggtc tgtacacata actcctgtag ttgccaaagc
    10501 atctgctgga gcagtagaat atatgccagt ttgcaaggta accaatatag ttaatacaat
    10561 aaaaagactc aaagaagaag ggctgtggat tgctgcagct gatatggatg gagaaacttt
    10621 ttataaacaa aatcttactg gtcctcttgg tgtagtaatt ggaagtgaag gttttggaat
    10681 atctagattg gttaaacaaa actgtgactt tatagtaaaa atgccaatga taggtaatgt
    10741 tacatcttta aatgcatctg tagcaggtgg aatactttta tatgagatat tcagacaaag
    10801 actggataaa agtaaatagg catgaaaaaa aatataaatc attacttaat aatagatggc
    10861 tacaatatta taaatgcgtg ggacaatttg aaagaactgg ctaaagagga cttagaagat
    10921 tctcgagaaa agcttattga tgacattata gaattttctg agtttatggg ttataagact
    10981 ataatagttt ttgatgctta taatgttaaa aattctcgag aaaaagttga aaaaagaaaa
    11041 cacataacaa tagtttatac aagagaacac caaacagcag atagttatat agagaaattt
    11101 ataacttctt tatcaaagta tgatgatgta aaagtagcca ctaatgacta tgcagaacag
    11161 caaatgattt taggaaaagg tgctactaga atgtctgcaa gagaattaaa gttagagtta
    11221 gatcgttcca agaataaaat gaaggaaaaa aatatcggtt tgaggaaaaa aattcagcga
    11281 aattggttgg aggaaaggtt agataaagaa acattgtcga aacttgagaa cattcgtaga
    11341 aagcgttgaa attactaggt tctttagact ataatatacc tataaatttg ttttatatat
    11401 gggggagatt tacatgttgg tagcaaaaga aaaaagttat gagttagtgg acaattgtca
    11461 gcaggatgag tataacattg tattaagagc aagtgaaggg gataaaatag cactagagta
    11521 cattattact aaatacagaa attttgtaaa ggcaaaagca aagtcatatt ttttaattgg
    11581 agcagataaa gaagatataa tacaagaagg aatgataggc ctttataagg cagttagaga
    11641 ttttgatgga agtaagacca attcatttaa atgttttgcg gagatatgta taacaagaca
    11701 aataataact gctataaaaa ctgcaacaag acaaaaacat atacctttaa attcatatgt
    11761 ttctttgaat aaaccaatat atgatgaaga gtcagacaga acacttttag atattatagc
    11821 tacaagtata gtaacagatc cagaagagct tataattagt aaagaagagt taaaaaatat
    11881 agaatcaaaa atgaacgaat tgttaagtga cttggaatta gaagtactag aattatattt
    11941 aaatggaaag tcataccagt ttatagcgga caagcttaaa agagatgtaa agtcaataga
    12001 taacgcttta caaagggtta agagaaaatt agaaaaacat cttgaaaaca gaaatgatta
    12061 atagtaaaat acttaccata agcttattgt gagcaaaatt aatttatatc gaaagataag
    12121 ggaggaaatt caaaatggct aaagctaaat acgaaagaac aaaacctcat gttaatatag
    12181 ggacaatagg acacgtagac cacggtaaaa ctacattaac agcagcaata acaaaaacat
    12241 tatatgacag atatcaatta ggagaagcag tagatttcgc aaacatagat aaagctccag
    12301 aagaaagaga aagaggaatc acaatatcaa cagcacacgt tgagtatgaa acaccaaata
    12361 gacactatgc acacgttgac tgcccaggac atgctgacta cgttaagaac atgataacag
    12421 gagcagcaca aatggacgga gcaatattag tttgttcagc aacagatgga ccaatgccac
    12481 aaacaagaga gcatatacta ttatcaagac aagttggagt accatatata gtagtattct
    12541 taaacaaatg tgacatggta gatgatgaag agttattaga gttagtagag atggaagtaa
    12601 gagatttatt aacagaatat gatttcccag gagatgacac tccaatagta agaggttcag
    12661 cattaat
//
LOCUS       DAELLV010000101        12612 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.101, whole genome shotgun sequence.
ACCESSION   DAELLV010000101 DAELLV010000000
VERSION     DAELLV010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12612
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.101"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            88..175
                     /locus_tag="KPD58_002170"
     tRNA            88..175
                     /locus_tag="KPD58_002170"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:122..124,aa:Leu,seq:caa)
     gene            complement(362..805)
                     /locus_tag="KPD58_002171"
     CDS             complement(362..805)
                     /locus_tag="KPD58_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265934.1"
                     /translation="MKKNMLYVGIIYFICGILLVLLATFTEFSFEAFIWGLSGAALGP
                     GVCMILQYMYWSKPERAVDYEEKIKNQRIEMNDERRIMLRDKAGRITNQIMCYVLVIL
                     IFIVSILSVFSVMVISKWVLVVLGLLILFQFICSVVVYNKLDKRL"
     gene            complement(808..1005)
                     /locus_tag="KPD58_002172"
     CDS             complement(808..1005)
                     /locus_tag="KPD58_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0265935.1"
                     /translation="MKNRLEEIRKERGIKQEELASILQVSRQTIGSLENGRYNPSIIL
                     AFKIARYFDMSIEEIFIYEEE"
     gene            complement(1197..2063)
                     /locus_tag="KPD58_002173"
     CDS             complement(1197..2063)
                     /locus_tag="KPD58_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG0265936.1"
                     /translation="MEWINKLNQAITYIEENLESGVDYAEAAKIACCSTFHFQRMFSY
                     IAEVPLSEYIRRRRMTKAAFELQNSNIKILELSEKYGYDSPTSFNRAFQNIHNISPSA
                     ARAKGVVLKAYPKMTLSIYVKGNIEMQYRIVEKKGFRIVGIKESMNMIVEECFEKVPK
                     LWAKCIKNGTIDKLSELINNEPCGLLGVSVCTNSKGLDYYIAAPTDKPVLEDTHEYLI
                     PSGTWAVFECVSPMPNALAIQELQKRIITEWLPSSGYVYANLPDIELYPNGDINAPDY
                     TTEVWIPIQKPS"
     gene            complement(2289..3158)
                     /locus_tag="KPD58_002174"
     CDS             complement(2289..3158)
                     /locus_tag="KPD58_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG0265937.1"
                     /translation="MDYKTIILNRKSVREYKNTDIKEEYLSEIKKYSESCKKLVPEID
                     VDVRIMNRSEVFNNLDKVAGYKGNMIDSPSYIIILSDIKDNYIENSGYIGESIIFKVT
                     ELGIGSCWVTFEDGKKVKEKLGINSDKEVTGIIAIGYGENVSNAKVLNATKTGQNYSK
                     SDMQVVTDKGSTRLGVEDVVYMNEWGNVASIEILEERALLDAFHYARLAPSTLNRQPW
                     RFIVDGGTVVLAVRKDGHTNLYEEKIDIGIVMLYFATIISATMFDLKWNLGTPDKDYK
                     VPEDYKIVGYCNI"
     gene            complement(3664..3885)
                     /locus_tag="KPD58_002175"
     CDS             complement(3664..3885)
                     /locus_tag="KPD58_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265938.1"
                     /translation="MNVNNHKCPECGEHSKKFEIVEENEDCVIFECYTCGTISILKYC
                     ENCKEFVFVNIPNNVETYEYCCGKCNNKI"
     gene            complement(4387..5367)
                     /locus_tag="KPD58_002176"
     CDS             complement(4387..5367)
                     /locus_tag="KPD58_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265939.1"
                     /translation="MGDTSRFYESVKNKYPKLKDGTRVHLWPKENIIIGPIYDEEDGY
                     KKSIGNVDSLSWKQIVILAECDGKNTTDDIINILSNRYRNIHNMKEKVMEFFMFYENV
                     YLTFEDDIQTLSSVFEITGNKSYITPLYFTIEIDGDNNTNDYFSEITSLLNCMYEKGC
                     RFIEVIGEDILKNKSMREIFQYMLDHFDLIVVTKDSFTIDRSLIKELDNYRHKVIWKV
                     YSKNDDTRIKLKEDIKITSLIKRGHTVVRGDREKAIKIEDMRFKDTKERFSKYGAEWS
                     HLYISSDGSVKSYSFQKDKRIYLGNIFNNSVEEIFLEMQNKIDKLEIVYQ"
     gene            complement(5531..6403)
                     /locus_tag="KPD58_002177"
     CDS             complement(5531..6403)
                     /locus_tag="KPD58_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG0265940.1"
                     /translation="MIIKVSMTVLVLILLYILYKYETQHIETTEYTIFNKKIPKEFDN
                     FKIVQVSDLHNKVFDKNNKVLIDKIESLNPDVIFITGDLVDGENKNFQVALDLIDNLV
                     EKYEVYHIIGNHEQKSLIKKYKHLYKDYFKELYSKKIINMENESVRIYRGNSHLNLYG
                     LIIPLECYPYLFANNKKLELSDNFVEDNLGKVNENEYNILLAHTPFFFEKYAKWGADL
                     VLAGHVHGGIIRIPFVGGVLSPNREFFPKYDWGKYEKDNSTMILNKGLGGSKVLIRLN
                     CKPEIVKITFKYEK"
     gene            complement(6567..7118)
                     /locus_tag="KPD58_002178"
     CDS             complement(6567..7118)
                     /locus_tag="KPD58_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0265941.1"
                     /translation="MDNKFPNVGENLRLLRQEMGISLDKASKMTGVSKAMLGQIERGE
                     SSPTVSTLWKISSGFKINFTTLLNENTNTYEVIKKEEVEPIVEQKGNMKLYPIYPFSP
                     QRRFELFIIELEENCTHVSSTHSHVLEEYVLVIEGKLDLNVDGKTYILESGHSIRFDG
                     TLEHIYKNLNKGKTIFHNIEVYR"
     gene            complement(7138..8040)
                     /locus_tag="KPD58_002179"
     CDS             complement(7138..8040)
                     /locus_tag="KPD58_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBG0265942.1"
                     /translation="MSKKGRVVKVQHFSVNDGDGIRTTIFLEGCKLKCKWCSNPDSWS
                     NIVKLGVMKDKCVSCNRCIDVCPQNISSLFDRAQINNKCDLCGECIKVCLKDAICIMT
                     EEMSVEEIVEEVEKDFIFFFESNGGITFSGGEPTLQIDFLRELVDIFYDKGINIAIET
                     CGYFDWNKVNDVFEKIDHIFVDIKSMDDNIHKEYTGVSNKIILDNICRLSKLNKSMVI
                     RVPIIYGVNDSEENIRNTALFVKQNVPGGKMELLPYHKFGIDKYKALGLEDYIYEFDE
                     ICNNHMLKLKEIVELTGVKIIEYK"
     gene            complement(8055..10427)
                     /locus_tag="KPD58_002180"
     CDS             complement(8055..10427)
                     /locus_tag="KPD58_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HBG0265943.1"
                     /translation="MSQTNRIEAFRQEYINSKPMICCERARIFTESHKKTEGEAICIR
                     RAKAFLETCKELPIKIFENELIVGTAGKFRRTGILTPEFSWQWVDKEMDTFDKRTQDP
                     YVISKEQIEFIRKEIFPYWKGKSLEEVFLARIPEDTAKILVDTGIIDNDSKWRQAVGE
                     VTPDYQDILFVKGYKGIKEDADKKIKELDISVSENIEKIDFYKSVSIVAQGIMTLAQR
                     YSNLAKEMSKQETDEKRKLELIKISEICMNVPANPPTNFYEAIQFVWFVQLGGILSEN
                     PLALNLGRFDQYMYPYYENDAREGKITESEAQELIEALWIKLSEWVWTISANTANYFA
                     GYNQFQNLTVGGKKRNGTDGTNDISYMCLKATESVKTHQPGLSVRVSQGAPDNFVMAV
                     AKLVKQGTGFPAIHSDSAGAQMLLQDGYDAEDARDWSNCGCVVPHFRKTGQWTSAVNI
                     NFAAALEYAMNEGKSRLTGEKMGLDTKNITEFTSFEELKDEFLKQLAYLVKSSVIGTT
                     VAQQIHKEMVPRPFLSTCVDGCLDKGVDLSKGGAKYNIGPVLTGIGLGVVSNSLAAIK
                     KLVFEDKVTTLEELTKALNNDWEGYEELRKLALDVPKYGNDNDYVDSLAIEVSDFYYT
                     ETRKYKDIFGSKFNSAFMGISNYVPTGKIVGATPCGRKATKPLTEGVSPFVGTDTTSP
                     LAAMKSASKINHDVHTGGTLLNLRLNQDLVETERGLRNLTSMIKSYFALGGFHVQFNT
                     ISNDTLLKAQENPEEYKDLLVRVAGYSTQFVNLSREMQDAIIARNSHSNF"
     gene            complement(10648..11337)
                     /locus_tag="KPD58_002181"
     CDS             complement(10648..11337)
                     /locus_tag="KPD58_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG0265944.1"
                     /translation="MKVKRNINRVAIIVALNLSLALMVGCMSPVSDKNLSSSEQISKD
                     SLKEQVEDKKDESIELFNNYSQKINDAKGREEVKKDLSQRFSKAIIDSGYSITNSKSD
                     GSFVVSINGAEKPPEKNIRSLFYSMSEDDKEGKVTIDILCAKEYPEDDKLSESDKYVK
                     FIYNLFKSLTDTNLTEKEIFSMVEEDFNKGVGVVELPYMKDIHVEVNKVKQSTKVLKL
                     SLVYKFDISNH"
     gene            complement(11578..>12612)
                     /locus_tag="KPD58_002182"
     CDS             complement(11578..>12612)
                     /locus_tag="KPD58_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG0265945.1"
                     /translation="FAITGNVISSKESLLVPYLESIKIQIYSNLIIILIYLIFSFFRK
                     SIKLISQDKRNYVYLLFALLVNTMNMLVNYVLKKLERFGSLHSEGYYFDNFVNPKLVG
                     ASSIFLILLFKEIIKENRLKSQAELIKNKLDMQYAHYLSIQESHMRVKKLYHDINNHI
                     YCIDNLRNNSKEINEYVNNLKDEIKTFKYIYNTGNMILDIIINEKSEVCLKKGIKFTC
                     SINFSKVNFVKPIDVSSIFSNILDNAIEACDKIVDENINKYIRIKGTITRSFFVLKCE
                     NSKLNQLTFNKNILLTNKMDKFVHGIGIQSIKSSLQKYNGELLFENSIDKFILNIYIP
                     LDQDTDSWGD"
BASE COUNT         4152 a         2198 c         1214 g         5048 t
ORIGIN      
        1 tttaatattc ccataataat aatagtatat taaaattaaa tatgttgcaa acaatatatt
       61 tttttgctat aataatatac atgacatgcc cgagtggtgg aattggtaga cgcagtggac
      121 tcaaaatcca ccggactaat actccgtgcc ggttcgagtc cggcctcggg caccattaaa
      181 aactcctaaa gctatttatt caatagatta ggagtttttt tatttttgat ttaaatttta
      241 agttattttt tgaaattact gtgtcttttt aaaatttata taattatata aatttatctt
      301 ctagcattaa tttaaaaact aatttacttt tatataattt tctattttat atcataactt
      361 attacaacct tttatctaat ttattgtata caacaacact acaaataaat tgaaataaaa
      421 taagcaatcc aagaacaact aaaacccact tgcttattac cattacagaa aatacactca
      481 atatagaaac aataaatata agaattacaa gaacatagca catgatttga ttagtaatac
      541 gtccagcttt atcacgtaac ataattcttc tttcatcatt catctctatc ctttgatttt
      601 ttatcttttc ttcataatct actgctcttt caggtttact ccaatacata tattgaagaa
      661 tcatacatac acctggacct aaagcagctc cagataatcc ccaaataaaa gcctcaaatg
      721 aaaactcagt aaatgtagcc aataaaacaa gtaaaattcc acaaataaaa tatataattc
      781 caacatataa catattcttt ttcattttta ctcctcctca taaataaata tttcttcaat
      841 actcatatca aagtatctag ctatcttaaa ggctaagatg atagaaggat tatacctacc
      901 attttctaat gaacctatag tttgtcttga tacttgcaaa atagaagcaa gttcttcttg
      961 ttttatgcct ctttcttttc ttatttcttc taatctattt ttcacatcta tcccccttta
     1021 atatctcaac tacggaaagt ctactttcca taattataca actatattcc atttatgtaa
     1081 agcttacttt ccatatttac aaaaaaataa cagactattt tcagcctgta attttagaat
     1141 ttcatgttgt ttattaattt taatgttatt tattaattcc atattattta ttaattttaa
     1201 cttggtttct gaattggaat ccaaacttct gtagtataat caggagcatt gatatcgcca
     1261 ttgggataaa gttcaatatc tggtaaatta gcatacacat acccagaact tggtaaccac
     1321 tcagtaataa ttcttttttg caattcctgt attgccagag catttggcat tggagaaaca
     1381 cattcaaata ctgcccatgt gcctgatggt attaagtatt catgagtatc ttctaataca
     1441 ggcttatctg ttggagcagc aatataataa tctaaaccct tactatttgt acacacacta
     1501 actccaagca atccacaagg ttcattattt attaactctg aaagtttatc aattgttcca
     1561 ttttttatgc atttagccca caacttagga actttctcaa agcactcttc tacaatcatg
     1621 ttcatacttt cttttatacc aacaatacga aaccctttct tttctacaat acgatattgc
     1681 atttctatat ttccttttac ataaatgctt aatgtcatct taggatatgc ttttaaaaca
     1741 acaccctttg ctctagctgc agatggagaa atgttatgta tattttgaaa agctctatta
     1801 aaagatgttg gtgaatcgta accatacttc tcagatagtt ctaaaatttt gatgttactg
     1861 ttctgaagtt caaatgcagc cttagtcata cgtcttcgtc taatatattc agataatggt
     1921 acttctgcaa tatatgaaaa cattctttga aaatgaaagg ttgaacaaca tgcaattttt
     1981 gctgcttctg cataatccac tccactttcg agattttctt caatataagt aatagcttga
     2041 tttaatttat ttatccattc catttgatat tacctccaag caaattatac agtttaataa
     2101 aataatatac ctctctattc atgcacaaaa ttgtaagcat ttaaatttat aagcattttt
     2161 caatcaatac aataaatata aaaattgtat tatacaataa aaaagttcaa taacattata
     2221 ttgtaaaatg ttattgaact ttttataaaa attaatttaa gtgtagttaa attaagtatt
     2281 acactatctt atatattaca atatcctact atcttatagt cttctggcac tttataatct
     2341 ttgtctggag tacctaaatt ccattttaaa tcaaacatag ttgcacttat tattgttgca
     2401 aaataaagca taactatacc aatgtctatt ttttcttcat ataaattagt atgaccatct
     2461 tttctaacag ctaaaactac agtgcctcca tcaactataa atctccatgg ttgtctattt
     2521 aatgtagatg gagctaatct tgcatagtga aatgcatcaa gaagtgctct ttcttctaag
     2581 atttctatac ttgcaacatt tccccattcg ttcatataaa caacatcttc tacacctagt
     2641 ctagtagacc ccttatctgt tacaacttgc atatcagatt ttgaataatt ttgcccagtt
     2701 tttgtagcat ttaatacttt agcattactt acattttctc catatccaat agctataatt
     2761 cctgttacct ctttatctga attaattcca agtttttctt ttaccttttt tccatcttca
     2821 aaagtaaccc aacaagaacc aattccaagt tctgttactt taaagattat actttctcct
     2881 atgtaccctg aattttcaat gtaattatct tttatgtcag ataatattat aatataactt
     2941 ggagaatcta tcatatttcc tttgtaccct gctactttat ctaaattatt aaaaacttca
     3001 cttctattca taattcttac atcaacatct atttctggaa ctagtttctt acaagattct
     3061 gagtatttct taatttcact aagatattct tctttaatat ctgtgttctt atattctcta
     3121 acagactttc tatttaatat gattgtttta taatccataa ttttatcctc cccaaactag
     3181 tattgtctta attatatacc cattaatata ttattttact catttattta ataaataaag
     3241 attacaattt tgtaattatt atttattaat aatattccac ttttacatta ataatgacca
     3301 gttgtgcaaa atccattgcc aaccaccaaa taaaaacata ttataaaggt aatataaaat
     3361 aacaaacaaa tcttaacaaa aaattaaact ttttttacac gaaagaaggc ttcccgttgc
     3421 cttctttatt tttataaaaa aataagctgt ttctaaaatg atttcacatt tttgaaacag
     3481 ccaagttatt ctataatgac aactataatt tgaggatgtg agggagagtt gtcattataa
     3541 atttagggta attatattat gttttaagct agttctttct atatttatat aatacatttt
     3601 atatatgaat tttatatgag attggtataa atgatatatg aaattacata tgtttatatt
     3661 atattaaatt ttattattac attttccaca acaatactca tatgtctcca cattatttgg
     3721 aatatttaca aatacaaatt ctttacagtt ctcacaatac tttaatatag atatagttcc
     3781 acaagtgtaa cattcaaata ttacacaatc ctcattttcc tctactattt caaacttttt
     3841 actatgttca ccacactctg ggcacttgtg attatttaca ttcataaata ctcctcctta
     3901 ttagtatagt tacatgtcaa aaacttaatt aatgtattct atattactta ttatatattc
     3961 tataataaat catattctaa tataataaaa caaattaaat aaaaaaaaaa ccataaattt
     4021 taaatttatg gattaattct tgtaatgata actctatttg aggatgtgag ggagggttat
     4081 cattacaata gggtattata ttatggtttt taagctagtc tttctacacc tcatatgata
     4141 catatgctat atgaatttta tatgattttt acataaatat tttatgaaat ttaaattttt
     4201 tagtcatttt gaaatttgta aaaagttctc taaaaatcca cagcatcata aacagccaaa
     4261 atttaagtaa aacatcataa aaaaatttgt ccaataattt taacaagtaa tctcacctaa
     4321 ttataattat tgacacaaac ttaaataaac aatctataaa tcttctataa atcttaaact
     4381 ttccccctat tgatacacaa tttctagttt gtcaatttta ttttgcattt ctaagaaaat
     4441 ttcttcaaca gaattgttaa atatattgcc taaatagatt cttttatctt tttgaaatga
     4501 ataagatttt acactaccat cagaagaaat atataaatga ctccactctg ctccatattt
     4561 tgaaaacctc tcctttgtat ctttaaacct catatcttct atttttattg ctttttctct
     4621 gtctcctctt actacagtgt gacccctttt tattaaactg gttattttta tatcttcttt
     4681 taatttaata cgtgtatcat cattttttga atatactttc catatcactt tatgacgata
     4741 attatctaat tcctttatta agcttctatc aatagtaaaa ctatctttag ttaccacaat
     4801 taaatcaaaa tggtctaaca tatattgaaa tatttctctc atacttttat tttttaaaat
     4861 atcctcacca ataacctcta taaatctaca gcctttctca tacatgcagt tcaataaact
     4921 tgtgatttca gaaaaataat catttgtatt attatctcca tctatttcta tagtaaagta
     4981 caaaggagta atgtatgatt tatttcctgt tatctcaaat acacttgata gtgtctgtat
     5041 atcatcttca aacgttaagt acacattctc ataaaacata aaaaattcca taactttttc
     5101 tttcatatta tggatatttc tatatctatt ggataatata tttattatat cgtcagtagt
     5161 atttttccca tcacattcag ccagtataac aatttgcttc caacttaagc tatccacatt
     5221 tccgatactt tttttatatc catcttcttc gtcataaata ggaccaatta taatgttttc
     5281 ttttggccac agatgaaccc tagttccatc ttttaatttc ggatatttat tttttacaga
     5341 ctcataaaat ctacttgtat cacccatgat tctttactac cccccatagt ttaaatattc
     5401 tatgctaaaa atagcacttt atttttatta attcaacaat tattcaaaat tggtcattat
     5461 atatgtttaa atggatttaa catcaacctt atttatatgt tattgatgtt tatatatgtt
     5521 atataaaatc ttatttctca tacttaaatg taatcttaac tatctctggt ttacaattaa
     5581 gcctaattaa aacttttgag cctcctaagc ctttatttaa aatcatagtg ctattatctt
     5641 tttcatattt tccccagtca tacttaggaa agaactctct atttggagat aatacacctc
     5701 caacaaaagg tattcttatt atacctccat gaacatgtcc tgctaaaacc aaatctgctc
     5761 cccattttgc atatttttca aagaaaaatg gtgtatgtgc taaaagaatg ttatattcat
     5821 tttcattaac tttacctaga ttgtcttcta caaaattgtc acttagttct aattttttgt
     5881 tattagcaaa taaatatgga tagcattcca atggtataat taaaccatat aaattaagat
     5941 ggctattacc tctatatatt cttacagatt cattttccat atttattatt ttctttgaat
     6001 atagttcctt aaaataatcc ttatatagat gtttatactt ttttattaaa gatttttgtt
     6061 catgatttcc aattatatga tatacttcat atttttcaac caaattatct attaaatcta
     6121 atgcaacttg aaaattttta ttttctccat ctactaaatc tcctgttata aatataacat
     6181 ctgggtttaa tgattcaatt ttatctatca ataccttatt atttttatca aacaccttat
     6241 tatgtaaatc actcacttga actattttaa agttatcaaa ttcctttgga atttttttgt
     6301 tgaaaatagt atattctgtc gtttctatat gctgagtttc atacttatac agaatataca
     6361 ataatattaa tactaatact gtcattgaaa cctttataat catattctct tcctctttat
     6421 tctattttgt taatattatt ataatgcttt tatgtttttt ttaaaacaat aacatcatca
     6481 catacatata aatcttttcg acattataaa aataagttat ccttttctaa aattttcatt
     6541 tattttagaa aaggataact ataaaattat ctataaacct ctatattgtg aaatatagtt
     6601 tttcctttat ttaaattttt atatatatgc tctaaagttc catcaaacct aatagaatgt
     6661 ccagactcta aaatataagt tttcccatcc acattaaggt caagtttacc ttctattacg
     6721 agtacatact cttctaaaac atgactgtgt gtagatgaaa catgagtaca gttttcctca
     6781 agctcaatta taaaaagttc aaaccttctt tgagggctaa aagggtatat tggatataac
     6841 ttcatgttcc ctttttgctc aacaataggc tctacttctt ctttctttat aacctcatag
     6901 gtatttgtat tttcatttaa tagtgtagta aaatttattt taaatccaga tgaaattttc
     6961 cataaagtag atactgtcgg acttgattca cctctctcaa tctgtccaag cattgcttta
     7021 ctgacacctg tcatctttga agctttgtct aaacttatgc ccatttcttg tctcaataac
     7081 cttaaatttt ctcccacatt aggaaattta ttatccatgt taaaatcctc ctgatagcta
     7141 tttgtattct atgattttta ctcctgtaag ttctactatc tcttttaatt ttaacatatg
     7201 attgttacaa atttcatcaa attcatatat ataatcttca agccctaatg ccttatattt
     7261 atcgattcca aatttatgat atggaagtaa ctccatcttg cctcctggaa cattttgttt
     7321 gacaaataat gcagtatttc ttatattttc ttcactatca tttactccat aaataattgg
     7381 cactcttata accatagatt tattcaactt tgaaagccta catatattat ctaatataat
     7441 tttattactt actcctgtat attctttatg aatattatca tccatagatt ttatatctac
     7501 aaatatgtgg tctattttct caaatacatc atttacctta ttccaatcaa aatatccaca
     7561 agtttcaatt gcaatattta tgcctttatc ataaaaaata tcaactaatt ctcttaaaaa
     7621 atcaatctga agtgtaggtt cacctccaga aaaagttatc cctccattag attcaaagaa
     7681 aaatataaaa tctttttcaa cttcctctac tatttcttca acactcattt cttcggtcat
     7741 tatacaaatg gcatctttaa gacatacctt gatacactct ccacataaat cacatttatt
     7801 atttatttgc gctctatcaa atagtgaact tatattttga ggacaaacat ctatacatct
     7861 attacaggat acacatttat ctttcataac acctaatttt actatgttac tccaggaatc
     7921 tggattagaa caccacttac attttaattt acatccttct aaaaatatag tagtccttat
     7981 tccatctcca tcatttactg aaaaatgttg tacttttaca actctacctt ttttactcat
     8041 aatctacctc ataattaaaa attgctatgt gaatttctag ctattatagc atcttgcatt
     8101 tctcttgata aattaacaaa ttgagtacta tatccagcaa ctctcactaa taaatcttta
     8161 tattcttctg gattttcttg agcttttaat agagtatcat ttgatatcgt attaaattgt
     8221 acatggaatc ctcctaaagc aaaatatgat ttaatcatag atgttagatt tctaagtcct
     8281 ctttcagttt ccaccaaatc ttgattcagt cttaaattta gaagtgttcc accagtatga
     8341 acatcatgat ttatttttga tgcagatttc attgctgcta gtggacttgt tgtatcagta
     8401 ccaacaaatg gagaaactcc ctctgtcaaa ggttttgtcg cttttcttcc acaaggagtt
     8461 gcgcctacga tttttccagt tggtacatag tttgatatac ccataaatgc actattaaat
     8521 ttagagccaa aaatatcctt gtattttcta gtttcagtat aataaaaatc agaaacttca
     8581 attgctaatg aatctacata atcattatca tttccatact taggaacatc caacgcaagt
     8641 tttcttaatt cttcataacc ttcccaatca ttatttagtg cttttgttaa ttcttctaac
     8701 gtagtcacct tatcctcaaa tactaatttt tttatagctg ccaatgaatt tgaaactaca
     8761 cctaacccta taccagttaa tacaggacct atattatatt ttgctcctcc tttacttaaa
     8821 tcaactccct tatccaaaca tccatctaca caagtagata aaaatggtct tggtaccatt
     8881 tctttatgaa tttgttgagc aacagtagtt cctataacag aactttttac aagatatgct
     8941 aattgcttta aaaactcatc cttaagttct tcaaaagaag taaattctgt gatatttttt
     9001 gtatctaacc ccattttttc tccagttaat ctactcttac cttcattcat agcatattct
     9061 aaagctgctg caaaattgat atttacggct gaagtccact gtcctgtttt tctaaaatga
     9121 ggaactacac aaccacagtt actccaatct ctagcatctt ctgcatcata tccatcttgt
     9181 aataacattt gtgcccctgc actatcgctg tgtatagcag ggaaaccagt tccttgtttt
     9241 accaatttag caactgccat tacgaaattg tctggtgctc cttgactcac tctaacactt
     9301 aatcctggtt ggtgagtttt tacactttct gtagccttta aacacatata tgagatatca
     9361 ttcgttccat cagtaccatt tctcttttta ccaccaacag taagattttg gaattgatta
     9421 tatcctgcaa agtaatttgc tgtatttgct gatatagtcc atacccattc tgataactta
     9481 atccaaagtg cttctatcaa ttcttgagct tctgattcag ttattttacc ttctctagca
     9541 tcattttcat aatatggata catatattga tcaaatctac ctaaatttaa tgccaatgga
     9601 ttttctgata gaataccacc taattgaaca aaccatacaa attgtattgc ttcataaaaa
     9661 ttagttggag ggtttgctgg tacattcata catatctctg aaatttttat aagttctaac
     9721 tttctctttt catctgtttc ttgcttactc atctcttttg caagatttga atatctttgt
     9781 gccaatgtca taataccctg tgcaactata gaaacagatt tgtaaaaatc tatcttttca
     9841 atattttctg aaactgatat atctaattct ttaatttttt tatctgcatc ttccttaatc
     9901 cctttatatc cttttacaaa caatatatct tgataatctg gtgttacttc accaactgcc
     9961 tgtctccact tagaatcatt atctattatt cctgtatcta ctaatatctt agctgtatct
    10021 tctgggattc ttgctaagaa aacttcttcc aaagattttc ccttccaata tggaaatatt
    10081 tcttttctta taaattctat ttgttctttt gatattacat atgggtcttg agttctttta
    10141 tcaaaagtat ccatttcttt gtctacccat tgccaagaaa actctggtgt caatatccct
    10201 gttcttctaa attttcctgc tgtacctact attaattcat tttcaaagat ttttatagga
    10261 agttctttac atgtctccaa aaaagcttta gctcttctta tacagatagc ttctccctca
    10321 gtttttttat gtgattctgt aaaaattcta gccctttcac aacatatcat aggtttagaa
    10381 ttaatatatt cttgtctaaa tgcttctatt ctattagttt gactcattta aaatccccct
    10441 ttggataata tatactacat ttttgtatgt tataacatac tttaattata ttatactgtc
    10501 ctttagcata aaatggaagc aaaataactt attttttttc atctaccttg aacacctata
    10561 tattaatact ttttttgtta aatttataaa atctaaaaaa attttctatg atatattttc
    10621 tatattgtta tagttatgtc taatatacta atgatttgaa atatcaaatt tgtatactag
    10681 acttagtttt aaaaccttag ttgattgttt aactttgtta acttcaacat gtatatcctt
    10741 catataagga agctctacaa cacctactcc cttattaaag tcttcttcaa ccatagaaaa
    10801 aatttctttc tcagttaagt tcgtatcagt caatgattta aataaattgt atataaattt
    10861 aacatattta tcactctcag ataacttatc atcttctgga tactctttag cacagagaat
    10921 atctattgtt acttttcctt ctttatcgtc ttcagacata gaatagaata aacttcgaat
    10981 atttttttca ggtggttttt cagcaccatt tatagatact acaaagctac catctgattt
    11041 tgagttagta atactataac cactgtctat tatagctttc gaaaatcttt gagataaatc
    11101 tttttttact tcttctctac ctttcgcatc attaatcttt tgtgagtaat tattaaatag
    11161 ctcaatagat tcatcttttt tatcttcaac ttgttctttc aatgaatctt tactaatttg
    11221 ttctgagcta cttaagttct tatcagatac aggagacata caccctacca tcaatgctaa
    11281 gctaagattt aaagccacaa ttattgccac tctatttata ttccttttta ctttcataca
    11341 caatcctccc atcacactaa ccgctctaaa gtattattca ttgcactaga atatatttat
    11401 attttcttta tattaaagat ttattattca atacatatca ccttcctatc caattcttat
    11461 aacttactat tagtaaaatt ataagctcct taatacagaa ataaggatgt tatcttttgt
    11521 tattaaaaga taacatcctt atttttttgt atcaatacat atgtcccaac tggtaactta
    11581 atcgccccaa ctgtcagtat cttggtccaa tggtatatat atatttagta taaatttatc
    11641 gatactattt tcaaataata attctccatt atatttttgt aaagaagatt taatactctg
    11701 aatacctatt ccatgtacga acttatccat tttgtttgtt aacaatatat ttttattaaa
    11761 agtaagctga tttagtttac tattttcaca tttaagtaca aaaaatgacc ttgttatagt
    11821 tcctttaatt ctaatatatt tattaatatt ttcatccact attttatcac atgcttctat
    11881 tgcattatcc aaaatattag aaaatatgct tgacacatct ataggtttta caaaatttac
    11941 ttttgaaaaa tttatagagc aagtaaattt aattcctttt tttaaacata cttcactctt
    12001 ttcgttgatt ataatgtcta atatcatatt tcctgtatta tagatatatt taaatgtttt
    12061 tatttcatct tttaaattgt taacatattc attaatttct ttgctattgt ttctaaggtt
    12121 gtctatacaa taaatgtgat tatttatatc gtgatatagt ttcttaactc gcatatgaga
    12181 ttcttgaata cttaaataat gcgcatattg catatctaac ttatttttta ttaattcagc
    12241 ttgagatttt aatctattct cttttattat ttctttaaac aatagtataa gaaatatact
    12301 actagctcct accaattttg gattaacaaa attatcaaaa tagtatcctt ctgaatgtaa
    12361 tgacccaaac cgttctaatt tttttaatac ataatttaca agcatattca ttgtattaac
    12421 taataaggca aataataaat atacataatt tctcttatct tggctaatta attttataga
    12481 ctttctaaaa aagctaaata taagatatat aagaataatt attaaattac tatatatctg
    12541 aatttttata gattctaagt aaggtactaa taaagattct ttagatgaaa taacattacc
    12601 tgtaattgca aa
//
LOCUS       DAELLV010000102        12457 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.102, whole genome shotgun sequence.
ACCESSION   DAELLV010000102 DAELLV010000000
VERSION     DAELLV010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12457
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.102"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            316..1251
                     /locus_tag="KPD58_002183"
     CDS             316..1251
                     /locus_tag="KPD58_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBG0265946.1"
                     /translation="MIRYYKTIDSKLEKLSFFEDGCWINLVEPNHSEINEISNLLNID
                     VESIESALDEEERSRIDVEDNHTLILIDIPVDESDSNSSHYTTIPLGIILTEEAIVTV
                     CDAQTKILNDFIVGHIKDFFTFKKTRFLLQILHKNAAYYLHYLRKINKMTIIIEREIY
                     KSMKNKELVQLLELEKSLVYFSTSLKSNELVLNKMVRTAGIKKYPDDEDLLEDVIVEN
                     RQALDMAKIYGDILSRIMDAFSAIISNNQNNVMQILTVVTLIFSIPTIISGFFGMNVI
                     NMPFSNNPNGFWIILLITSIICIVITFFMSRNKLL"
     gene            complement(1343..3214)
                     /locus_tag="KPD58_002184"
     CDS             complement(1343..3214)
                     /locus_tag="KPD58_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBG0265947.1"
                     /translation="MKNTKFSIKKIKNISNDPKVVHTKGALLEKMGKMESAIELYKSA
                     ALDNYVKSQYTLGNIYESKKQFKEAEKWYSMAYKSGSEDAAFDLGNMYYKLDGYEYAI
                     YWYEKIANLGYLQAQNNLGVCHFKMKDFVRCEKWLKTAADKNLGKACFNLGVLYTFLE
                     KDDEAYEYYKKGSVLLDDDAKYNLAILNGKKKDNKSTVSLYKQLYKSGHMKGCFNLGM
                     IMEINDNLEDAEKYYKKSADRDDVKSEYRLAYVYDRKEELGDAIYYYEKAIEKNHTLS
                     KYRLANLYNRENEIEKAKTYYKMAAEDDITEAKNNLAGIYFEEKDYENAIKYYEDAIA
                     VGCKSSLENLGDLYYQNQDIEKAISYYSRIPNNVSCQIKLGNIYEDLNNIEEAISWYK
                     KASENGDTRSSYRLGCIYESLGNTKNARKYFEMASSKNHMNARIHLGRIYFREGKLEE
                     SKMMFDTPANENNVYAQHMVGLIYDMFYKDYVNSKFWYEKARAQGCVESIYNLGQIYL
                     KLNDDAEAEKYYKEGIKYGSKKCEYMLAGLYYKKSLDMYISLANEEYDNCEEIVEGFP
                     NLNIDFDEVLIPPFKLQEEVIDEEEYVPIYILNIKESLDSMLEGLETEMIIDEEHDS"
     gene            3404..4330
                     /locus_tag="KPD58_002185"
     CDS             3404..4330
                     /locus_tag="KPD58_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBG0265948.1"
                     /translation="MNFSNIFIQVAVLFIIILVGYFVRKFNLLDDHCTSKLSTLTMTV
                     FLPSMIISSMQINFDSKMIQKILLLLFTSLIMYIVSIIIAFLLKYILKCNDKKDLGIY
                     QYIVVFSNVAFMGYPVIEAVLGHEAIFYTAIFNLPFNLFSFTLGIYLLSKGSTSRGFS
                     IKSLISPATIAIVIGLFLFVTGLRLPQFINEPLEMLGSITTPISMIIIGSLLANSSAL
                     DCFVNKKLYIVTFIRLLVLPVIVYFILKGWINDKMILAIPVVISSMPAAANTAIMANQ
                     YDSNITLASQCVFFTTLFSVISIPFISILLLS"
     gene            4469..4615
                     /locus_tag="KPD58_002186"
     CDS             4469..4615
                     /locus_tag="KPD58_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265949.1"
                     /translation="MDMDLLFKIVFAIVAIGVSVKIIKTVASLAFKIALILLIILFVY
                     RMFL"
     gene            complement(4720..5244)
                     /locus_tag="KPD58_002187"
     CDS             complement(4720..5244)
                     /locus_tag="KPD58_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0265950.1"
                     /translation="MSKPIDITIRPIRREDAQAFNEIRRTYSVMRNTLALMSDRVDKT
                     EAMLASLGENDYMFVAETNNNGEKDVIGFAGLHVNSSPRLRHSAEIGISVAENYQGRG
                     VGKKLMEQLLDIADNWIMLTRVGLEVIVDNEKGLNLYKKLGFEIEGTKKYAVIRDGKF
                     EDVYIMGRYNKNLI"
     gene            complement(5297..5521)
                     /locus_tag="KPD58_002188"
     CDS             complement(5297..5521)
                     /locus_tag="KPD58_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265951.1"
                     /translation="MIIKLTKDNFNFRFKQYEGMFFHAEVVEGMESIRYKITKDKEGT
                     ELKEFGSTHIKDNMIYVPQVEAYIDLNSIK"
     gene            5718..6842
                     /locus_tag="KPD58_002189"
     CDS             5718..6842
                     /locus_tag="KPD58_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HBG0265952.1"
                     /translation="MSKKTKYFSLILFPIALLLNFIASKVPNIVEKYYSQFIDKIIVQ
                     ILSKVSGIFPFSLYEITMYIIVISIFLFLCCTLSTIFNKKKKLNIYLKNSILNILSIV
                     SIVYFLFVVLWGINYNRIPLETTLINNYNLKNNTSIQSKQYSVKELTKLYKFLVIKAN
                     ETRKLTLQDKNGIVKSNTDYNGVINRAQLGYDNILDILPGVSGSYSKPKYIISSNLMC
                     YTGITGIYFPFTGEANVNIAIPDLYIPCTVEHEMAHQRGFASEDEANFIAYLTSIKHP
                     NIDFNYSGYILALNYTASALSKVDYNAYVDISAGISDSVRRDLKNESEFWQKYEGKIN
                     EISNEFNNSYLKANSVTEGTQSYGKMVDLLLTYYELYPYN"
     gene            complement(7316..8140)
                     /locus_tag="KPD58_002190"
     CDS             complement(7316..8140)
                     /locus_tag="KPD58_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265953.1"
                     /translation="MRYKKISSLPILVVALLITGTTTTFSLSTTNSEKTNVYLANTVS
                     TVTKKVSDIQSSKGLVYNENIPLFINNSKIQYDDTPYHWPSNVISLTNSSEKAIMDYE
                     ITCLAYDKNGKPLELYWDAQNVAADGEVGSVGFSPAGVDYGIVTGISPVSPKSYSHTY
                     RKMQQSPPQDIISMFEKQQGKAWVENWLKEWKQMEKEYAKQNAIAPGKNQNDAFLLFD
                     KWKQSTGEHGVKYIISCVKQVTFNDGSVWKNSAYENWLKSFQGKEVSNSVLENYYK"
     gene            complement(8488..9543)
                     /gene="ispG"
                     /locus_tag="KPD58_002191"
                     /gene_synonym="gcpE"
     CDS             complement(8488..9543)
                     /gene="ispG"
                     /locus_tag="KPD58_002191"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HBG0265954.1"
                     /translation="MSYERRKTREVSVGTVKIGGENPISIQSMTNTDTRDADATIAQI
                     KRLEEAGCDIVRVAVPDIKAAKNIAKIKSSVNIPIIADIHFDYKLALEAIEQGVDGIR
                     INPGNIGSIERVKMVVEKCKERNLKIRIGVNGGSLEKELLKKYGSATAEALVESAMGH
                     IKILEDLDFHNIVISLKSSDIYKTVDAYELISKKVDYPLHIGITESGSVHKGTIKSSI
                     GVGALLLKGIGDTVRISLTGDPVEEVIVGKQILRSLGLLNDKIKVISCPTCGRCNIDL
                     ISVVNEVEEKIGSMEKNITVAIMGCAVNGPGEAREADIGIAGGKGEGLLFKKGEIVRM
                     IDGNKLVDELLEEIEKL"
     gene            complement(9725..10729)
                     /gene="rseP"
                     /locus_tag="KPD58_002192"
     CDS             complement(9725..10729)
                     /gene="rseP"
                     /locus_tag="KPD58_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HBG0265955.1"
                     /translation="MTIIAALILFSIIVLIHELGHFIFAKRSGIKVNEFSIGMGPKIY
                     SVKKDTEYSIRALPIGGYVSMEGEDEEQISPNSFGNKSILQRFSTIVAGPIFNIILAA
                     ILLVPVFLYIGSPTTKLGKIMPDTPAQAVGLQVGDKINKINGNSVKTWDEVANIINTS
                     SGGELKLSITRDGSDKVVNVTPKNNNGKYEIGIQPQREKDFLGSIVNACKTTVDMTKQ
                     MLTFLGQMITGRVPGGIGNAVAGPVGVIGMVSDAARTGIINVVYLAAVISLNLGIVNL
                     LPIPALDGWRILMLLLEAVRGGKKLDPNKEGMINVVGFGALMLFMLFITYKDILRLFQ
                     "
     gene            complement(10742..11896)
                     /locus_tag="KPD58_002193"
     CDS             complement(10742..11896)
                     /locus_tag="KPD58_002193"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HBG0265956.1"
                     /translation="MKKISILGSTGSIGKQTLDVVRDNRDKFEIVAISANSNIELLLE
                     QIVEFKPKYVTVFEENKALKLKEMLPKNIEIEVLAGMEGLKIISSLDEVDVLLTAVVG
                     MIGLVPTLCAIKKGIDIALANKETLVTAGELVMKEAEKYNVNILPVDSEHSAIFQCLN
                     GENKKNIEKIILTASGGPFRGKKKGELANITKNEALKHPNWSMGRKISIDSSTLMNKG
                     LEVIEARWLFGVEQENIDVVVHPQSIIHSMVQYTDSSIIAQLGCPDMRLPIQYALTYP
                     DRMESSFERMNFSKFSTLTFEEPDLETFPCLKLAYECLKMGGTYSSVLNSANEVLVSE
                     FLEDKIGFYDIPYYIEKTLEVHSSISEPTLEEILETDRWSRAYVANLIKK"
     gene            complement(12060..12263)
                     /locus_tag="KPD58_002194"
     CDS             complement(12060..12263)
                     /locus_tag="KPD58_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0265957.1"
                     /translation="MISYAPLWKLLIDRKIKKMEFVNISGISISVLGRLGNDKSVSMD
                     TMEKICLALDCKIEDVVEIKKSI"
BASE COUNT         4156 a         2092 c         1294 g         4915 t
ORIGIN      
        1 tatatacttt atgtgtaaat tatggtaaaa atattattta atcaaatcaa aacttgattt
       61 atatacctgg aaaaggtatt atattaaaga gtgcttaaaa tacgcatttc ataaatagct
      121 atatataaaa taataaataa ggagagtgat gggcaatgga tactagtccg ttgcggagag
      181 aattttttac attttcatca cttaataata tctaaaatca aaactaatac atatattcag
      241 ttatataaat tagttaatta atagatacta ttaaatgatg agatttatat aaaactgaat
      301 aaaggatgtg ttgccatgat aagatattac aaaactatag attcaaaatt agagaaactt
      361 agtttttttg aagatggctg ttggataaat cttgttgaac ctaatcatag tgaaatcaac
      421 gaaatttcta atttattaaa tatagatgta gaaagcatag aatctgcact agatgaagaa
      481 gaacgctcaa gaatagatgt tgaagacaat catactctta tacttataga tataccagta
      541 gacgaaagtg actctaattc ttctcattat actacaatac ccttaggtat tatactaact
      601 gaagaagcta ttgtaacagt ttgtgatgca caaactaaga ttttaaatga ctttatagtt
      661 ggtcatatta aagatttttt tacatttaaa aagactcgct ttttgcttca aattcttcac
      721 aaaaatgcag cttattactt gcattactta agaaaaataa ataaaatgac aataataata
      781 gagcgagaaa tttacaaatc aatgaaaaac aaagaattgg ttcaattatt ggaattagaa
      841 aaatctttgg tatacttctc tacctcacta aaatctaatg aacttgtttt aaacaaaatg
      901 gttagaactg ctggtataaa gaagtatcca gatgatgaag acctattaga agatgttata
      961 gttgaaaata gacaggctct tgatatggct aaaatatatg gagatatatt gagtagaatt
     1021 atggatgctt tcagtgcaat tataagtaat aatcagaata atgttatgca aattttaaca
     1081 gtagttacac taattttctc aatacctaca ataatatcag gattctttgg tatgaatgtc
     1141 atcaacatgc cattttctaa taacccaaac ggattttgga taatacttct tattacctct
     1201 ataatatgta tagtgattac atttttcatg tcaagaaata agctattata aaatattaat
     1261 aaaaaagcca ttagaattaa ttgatttaca tttatcctaa tggcttttat atatttaaat
     1321 ctatataatc ttataatact ttttaactat catgttcttc atctataatc atttcagttt
     1381 caagcccttc tagcattgaa tctaaacttt cttttatatt tagtatataa attggtacat
     1441 actcttcttc atctattacc tcttcttgca acttaaatgg aggaattaat acttcatcaa
     1501 agtctatatt taaatttgga aagccttcta ctatctcttc acaattatcg tactcttcat
     1561 ttgctaaaga aatgtacata tctaggctct ttttataata aagacctgca agcatatact
     1621 cacacttctt acttccatat ttaatacctt ctttataata tttctcagct tctgcgtcat
     1681 catttaattt taaataaatt tgacctaaat tatatattga ttcaacacaa ccttgtgctc
     1741 tagccttttc ataccaaaat ttagaattta cataatcttt atagaacata tcataaatta
     1801 atcctaccat atgttgagca tacacattat tttcattggc aggtgtatca aacatcattt
     1861 tagactcttc caatttacct tctctaaaat aaattctacc tagatgtata cgagcattca
     1921 tatggttttt gctacttgcc atttcaaaat actttctagc attttttgta tttcctaagc
     1981 tctcatatat acaccccaat ctataactag acctcgtatc tccattttca gatgctttct
     2041 tataccaact tattgcttct tcaatattat ttaaatcttc atagatattt cctaatttga
     2101 tttgacaaga tacattattt ggaatcctcg aataatacga aatcgctttt tctatatctt
     2161 gattctgata atataaatcc cctagatttt caagggaact tttgcatcca acagctatag
     2221 catcctcata atatttaata gcattttcat aatctttttc ttcaaaatat atacctgcca
     2281 gattattttt tgcttctgtt atatcatctt cagcagccat tttataataa gtttttgcct
     2341 tctctatctc attttctcta ttatataaat tagcaagtct atacttagaa agtgtatgat
     2401 ttttttctat tgctttctca taataataaa ttgcatctcc cagctcttct tttctatcat
     2461 atacatatgc aagcctatac tcagacttca catcatctct atcagcactc tttttataat
     2521 acttctctgc atcttctaaa ttatcattta tttccataat catacccaag ttaaagcaac
     2581 ctttcatatg acctgacttg tacaactgtt tatataaact tactgttgat ttattatctt
     2641 tcttttttcc atttaatatt gctaaattat attttgcatc atcatctaaa agcacagaac
     2701 cttttttata gtattcatat gcttcatcat ctttctccaa aaaagtatat aataccccta
     2761 aattaaaaca agctttacct aaattcttgt ctgctgctgt ttttaaccat ttttcacacc
     2821 ttacaaaatc tttcatttta aaatggcata cacctaaatt attttgtgct tgtaaataac
     2881 ctaaatttgc tatcttttca taccaatata tagcatattc atagccatct aacttatagt
     2941 acatattacc taaatcaaaa gcagcatctt cacttccact tttatatgcc attgaatacc
     3001 acttttctgc ttctttaaat tgttttttac tttcatatat atttcctaaa gtatattgag
     3061 attttacgta attatccaat gcagcactct tatacaactc tattgcgctt tccatcttac
     3121 ccatcttttc aaggagggca cccttagtat gtacgacttt cgggtcatta gatatatttt
     3181 ttattttttt tatagaaaat tttgtatttt tcattattcg ctacctaccc attttataaa
     3241 atatattctc tcaaacatta ttatacatta taatctacaa ttaatgctat aatattgaat
     3301 ttttaccttt tgtcactata ttgtaacatt aaattttaaa ttttataatc attagatttt
     3361 attatgtaat tggtatataa tgtatatata ggggggacat tttatgaatt tcagcaatat
     3421 ttttatccaa gttgcagttt tatttataat tattttagtt ggatattttg tcagaaaatt
     3481 caacttatta gatgaccact gcacatctaa gctttcaact ttgacaatga cagttttttt
     3541 accatcaatg ataattagct ccatgcaaat taactttgat agtaaaatga tacaaaaaat
     3601 actattgtta ttatttacat ctttgattat gtatatagtt tcaattataa ttgcattttt
     3661 attgaaatac attttaaaat gtaatgataa aaaagatttg ggaatatacc agtatatagt
     3721 tgttttttca aatgttgctt ttatgggtta tcctgtaatt gaagcagttt taggtcatga
     3781 agcaatattc tatactgcaa tattcaattt gccttttaac ttattttctt tcactttagg
     3841 aatttatctt ttatctaaag gcagtacaag tagaggtttt tcaataaaat cacttataag
     3901 ccctgctaca atagctattg ttattggact ttttttattt gtaacaggac ttaggttacc
     3961 ccaatttata aacgagccac ttgagatgtt gggaagtata actacaccaa tatccatgat
     4021 tatcattgga agcttacttg ctaattcttc agctctcgat tgttttgtca acaaaaaatt
     4081 atatatagtc acttttataa gattattggt acttcctgtg attgtgtatt ttattctaaa
     4141 aggatggata aatgataaga tgattttagc tataccagta gtaatttctt caatgcctgc
     4201 tgctgcaaat actgctataa tggctaatca atatgactcc aatataacac tagcttcaca
     4261 atgtgtgttt tttacaacac tattttcagt tatatctata ccttttataa gtattttact
     4321 cttatcttga tttatttttt tatgtattat ataataacat attcaataaa ttacaaatat
     4381 aaacatttta ttgtagtata ttgatggaat tttttcctag atatttgtca ataatatcta
     4441 tatgtattat agaataggag gcttaaatat ggatatggat ttactcttta aaatagtatt
     4501 tgctatagtt gctataggtg tttctgtaaa aatcattaaa actgttgcta gtttagcttt
     4561 taaaatagcg ttgatattgc taataatttt atttgtatat agaatgtttt tgtaaacatt
     4621 taaaaataag ttttttaaca caatagagaa gtaaaataga ttatctttaa tgtaaatttt
     4681 ttattaaaga cactctattt tacttctcta ttagcttatt taaattaaat ttttattata
     4741 tcttcccata atataaacat cttcaaattt tccatctctg attacagcat attttttagt
     4801 tccctctatt tcaaaaccta gctttttata taaatttaat cccttttcat tatccacaat
     4861 tacttctaat cctactctag ttaacataat ccaattatct gctatatcta aaagttgttc
     4921 cataagcttc ttacctactc ctcttccctg ataattctct gcaacactta ttcctatttc
     4981 tgcgctatgt ctcaatcgtg gagaagaatt tacatgtaac ccagcaaaac ctattacatc
     5041 tttttctcca ttattattag tttcagctac aaacatataa tcattttctc ccaaagaagc
     5101 taacatagct tcagttttat cgactctgtc actcattaat gccaaagtat ttctcataac
     5161 actgtatgtt cttctaattt cattaaaagc ttgtgcatct tctcttctta taggtcttat
     5221 tgttatatct ataggcttac tcatattgga ttccccctca aacataatct ttatttttaa
     5281 ttaaaatttt acatctttat tttatactgt tcaaatctat gtatgcttct acctgaggta
     5341 catatatcat attatctttt atatgtgtac ttccaaattc ttttaattct gtaccttctt
     5401 tatccttagt tattttatat ctaatggatt ccataccttc aactacttca gcatgaaaaa
     5461 acataccttc atattgttta aatctaaaat taaaattgtc tttagtaagt tttattatca
     5521 tatttttatc tactccttat ataattgata tttttcttat tattagttgt atatatactg
     5581 cttattcata ttaatatatt ataacttatc atttatttag tataaagtta taaatagata
     5641 tatttatact tattttacaa tctttcttca attgatatat aatatgttta tataaactaa
     5701 tgtatgtgag gtgaaatatg agcaaaaaaa caaaatattt ttctttaatt ctctttccta
     5761 ttgctttgct tttaaatttt atagccagca aagttccaaa tattgttgaa aaatattact
     5821 cccaattcat agataaaata attgtacaaa tattgagtaa agtttctgga attttcccat
     5881 tttctctcta tgagattaca atgtacataa tagttatatc tatcttctta tttttatgtt
     5941 gtactttatc tacaattttt aataagaaaa aaaaattaaa catatattta aaaaattcca
     6001 ttttaaatat attatccatt gtatcaatag tctactttct ttttgtagtg ctttggggaa
     6061 taaactataa taggattcca ctagaaacca cacttataaa taactataat ttaaaaaaca
     6121 atacctctat ccaatcaaaa caatatagtg ttaaagagct tactaaatta tataaattcc
     6181 ttgtaattaa agcaaatgaa actagaaaac tcacattaca agataaaaat ggtattgtaa
     6241 aatctaatac agattataat ggtgtcataa atagagctca actaggatac gataatatct
     6301 tggatatatt acctggtgtt agtggtagtt attctaagcc taagtatatt atttcatcaa
     6361 atctaatgtg ctatacagga ataactggta tatactttcc ttttacaggt gaggcaaatg
     6421 taaatatagc aatacctgac ttatatattc cttgtacagt tgaacatgaa atggcacatc
     6481 aaaggggatt tgctagtgaa gatgaggcta attttatagc gtatcttact tctataaaac
     6541 accccaatat tgattttaac tattctggat atattttagc tttaaattat actgcttctg
     6601 ctttaagtaa ggttgattat aatgcatatg ttgatatttc tgctggcatt tcagattctg
     6661 tccgtagaga tttaaaaaat gaaagtgaat tttggcaaaa atatgaaggt aaaattaatg
     6721 aaatttctaa tgagtttaac aattcttact taaaagctaa tagcgttact gaaggtactc
     6781 agagttatgg taaaatggtt gatttattgc taacttatta tgaattatat ccatataact
     6841 aaacactaca attcaaaatt ttattcatat acagctgata ttaaatcagc tgttttctat
     6901 ttaaataaat tatttaactt caagcaatct tatataattt gatagtactt atactatctg
     6961 tataacattt caattctaac tattttagtt aaaacatttc tattatcttt tttaataaat
     7021 gttttaacta aaatttttaa aacataaaac ttccacatta tttcactatc tatataaaaa
     7081 tctaaaaatt cacatataaa attacataac taaaaaatca taaatttata gaaaacatat
     7141 attcaaaata aaaaagcatg tatcaaaact tgacacatgc ttataatctt atgtatatat
     7201 atcattttgt cacaataaaa atttacttgt aacactgata tttttaaaca aaatagtcct
     7261 tatttaatta aattaacatt aattaaattc gtactcattt tgctatcatt aaacattact
     7321 tatagtaatt ttctaataca ctatttgaaa cttccttccc ttgaaaactt ttcaaccaat
     7381 tctcatatgc tgagtttttc catacactac catcattaaa tgttacttgt tttacacaag
     7441 atataatgta ctttacacca tgctctccag ttgattgttt ccatttatca aacaatagaa
     7501 aggcatcatt ttgattctta cctggtgcta ttgcattttg ctttgcatat tctttctcca
     7561 tttgtttcca ttctttcaac caattttcta cccatgcctt gccttgttgt ttttcaaaca
     7621 tacttattat atcctgtggt ggtgattgtt gcatttttct ataagtatgt gaatatgatt
     7681 ttggagacac aggagatatg cctgtaacaa taccatagtc tacaccagct gggctaaatc
     7741 caacacttcc tacttcgcca tctgctgcca cattttgagc atcccaatat agctcaagtg
     7801 gttttccatt tttatcatat gctaagcatg taatttcata atccataatt gccttttcac
     7861 tgctatttgt taagcttata acattgctag gccaatgata tggagtatca tcatactgta
     7921 ttttgctatt atttataaat aatggaatat tctcattata aacaagacct ttactacttt
     7981 gaatatctga taccttcttt gtgacagtac ttaccgtatt tgctaagtat acatttgttt
     8041 tttctgaatt ggtcgtacta agagaaaatg ttgttgtcgt tccagtaatc agcaaagcaa
     8101 caactaaaat aggtaagcta gatatttttt tatatctcac aattcttcac ctctttttct
     8161 attaacttat aataaaacaa tttaccaaaa cattaaaatc tatgttaaca taatttacca
     8221 actgttctag taactatatc ctatcatagt tactatcaag tttcaatact attgatggaa
     8281 attttcacta tattaaatct ttttttaatt ttataaattt acaatatgat ttaactcata
     8341 catatatagt caaattagaa tataagtttc ttacatttaa tgaagtttat aaaataaaaa
     8401 atgactagat aattaaatac tgtaaataaa aacttattac aaaagtattt aaaaatctag
     8461 tcaattaata tatatatgaa ttgtatacta taatttttca atctcttcta gcaactcatc
     8521 aactaatttg ttaccatcaa tcattctaac tatttcaccc tttttaaata aaagaccttc
     8581 acctttaccc ccagcaatac ctatatctgc ctctctggct tcaccaggtc catttacagc
     8641 acagcccata attgcaacag tgatattttt ttccatacta ccaatctttt cttcaacctc
     8701 atttacaaca ctaattaagt ctatattaca tctaccacat gtagggcatg atataacttt
     8761 aatcttatca tttaaaagtc ccaaacttct taaaatctgt ttaccaacaa taacctcttc
     8821 aacagggtcg cctgttaaag atattctaac agtatctcca attcctttaa gaagaagtgc
     8881 tcctacccct atagaagact ttattgttcc tttgtgaaca cttccagatt ctgtaattcc
     8941 aatatgaaga ggataatcaa ctttttttga tattaattca tatgcatcaa cagttttata
     9001 aatatcggat gattttagag atataactat attatgaaaa tctaagtctt caagaatctt
     9061 aatatgtccc attgcacttt caactaatgc ttcagcagtt gcagagccat acttttttaa
     9121 aagttctttc tctaaagaac ctccattaac tccaattcta atttttaaat ttctttcctt
     9181 gcatttctca actaccatct taactctctc tatacttcca atattaccag gatttattct
     9241 tattccatcc acaccttgtt ctatagcttc aagggctagc ttataatcga aatgtatatc
     9301 tgcaataatt ggtatattta cactagactt tatctttgct atatttttag cagctttaat
     9361 atcaggaaca gcaactctaa ctatatcaca accagcttcc tctaatctct ttatttgagc
     9421 tattgtagca tctgcatctc ttgtatcagt atttgtcata gattgtatac ttataggatt
     9481 ttctcctcct atttttacag ttcctacact tacttctctc gtctttcttc tctcgtaact
     9541 catattccac ctctatatat tattttatat tgaatctctt taaatattaa aatattataa
     9601 cctacatgta tattataaat taaaaagcta tctagttaaa taagaataaa tttcatatat
     9661 aaaaattaat ttattcttat ttaaaactac atagcctatt ttcttaaaat ctctaaaaac
     9721 taaatcattg aaataatctt agtatatctt tatatgtgat aaaaagcata aataacatta
     9781 aagctccaaa accaacaaca tttatcatgc cttctttatt tgggtcgagt tttttaccac
     9841 ctctaacagc ttctagtaac agcataagta ttctccatcc atcaagtgct ggtattggta
     9901 atagatttac tatacctaaa tttaagctta tgacagctgc taaatacact acatttatga
     9961 tgcctgtacg agcagcatct gataccatac caataacacc tactggtcct gctacagcat
    10021 tgccaatccc tcctggtaca cgtcctgtaa tcatttgacc tagaaatgtt agcatctgtt
    10081 ttgtcatatc tacagtagtt ttacatgcat ttactattga ccccaaaaag tctttttctc
    10141 tttgtggttg aatccctatt tcatactttc cattgttatt tttaggtgtt acatttacaa
    10201 ctttatcact tccatctcta gttatactaa gttttaattc tccaccagaa gaagtattta
    10261 ttatatttgc aacttcatcc caagtcttaa cagaattacc attaatttta tttatcttat
    10321 ctcctacttg aagccctaca gcctgtgcag gagtatctgg cattatcttt cctaatttag
    10381 tcgttggaga acctatatac aaaaatactg gaactaaaag tattgctgct aatattatat
    10441 tgaatattgg tcctgctaca atagtactaa atctttgtaa tatagacttg tttccaaaag
    10501 aatttggact tatttgttct tcatcttcac cttccatgct gacatatcca ccaattggaa
    10561 gtgctcttat agaatactct gtatcttttt ttacactata tatcttaggc cccataccta
    10621 tagaaaactc atttactttg ataccacttc tttttgcaaa tataaaatgt cctaactcat
    10681 gtattaatac aattatacta aatagtatta atgcggctat tatagtcata aattcacctt
    10741 cctatttttt tatcagattt gcaacataag ctctactcca tctatctgtt tctaagattt
    10801 cttctaatgt tggttcactt atgctactat gaacttctaa agtcttctct atgtaatatg
    10861 gtatgtcata gaaaccaatt ttatcctcta aaaattcact tactaaaact tcatttgctg
    10921 aatttaaaac agaggaataa gttcctccca tttttaaaca ctcatatgcc aattttaaac
    10981 atggaaaagt ttctaaatct ggttcttcaa aagttaaagt actaaatttt gaaaaattca
    11041 ttctttcaaa gctactttcc attctatctg gatatgtaag agcatactgt attggtagtc
    11101 tcatatctgg acatcctaat tgagcaatta ttgagctatc tgtatactgc accattgaat
    11161 gaattatgct ttgtgggtga actactacat ctatattttc ttgttctact ccaaataacc
    11221 atctagcttc tatcacttca agtcctttat tcataagcgt tgaagaatca atacttattt
    11281 ttcttcccat actccaattt ggatgcttca aagcttcatt tttagttata tttgcaagtt
    11341 ctcccttttt ctttcctcta aatggaccac cagatgctgt aagtattatc ttttctatat
    11401 tttttttatt ttctccattt agacattgaa atatagcact atgttcacta tcaacaggaa
    11461 gaatatttac attgtatttt tctgcttctt tcattacaag ttcaccagca gttaccaacg
    11521 tttctttatt cgctaatgct atgtctattc ccttctttat agcacaaaga gttgggacta
    11581 atcctatcat acctactaca gcagttaaaa ggacatccac ttcatcaagt gatgatatta
    11641 tttttaatcc ttccattcct gctaaaactt ctatttctat atttttaggc aacatttctt
    11701 ttaatttcaa tgctttattt tcctcaaaaa ctgttacata tttaggttta aactctacta
    11761 tctgttctag caataattca atattactgt tagctgatat tgcaactatc tcaaacttat
    11821 ctctattatc tctaactaca tccaaagttt gtttacctat agagcctgta gaacctaaaa
    11881 ttgatatctt tttcatataa taatcctttc taataataaa tataccctgt caaaacatta
    11941 taacactact aaaacaatta tttttactat tcatagaaat ttcatatatt attccacaga
    12001 ttaaaattaa tctattattt tttgtatttt gtggaatatt ataaaatatt ctagtattat
    12061 taaatgctct tttttatttc cacaacatct tctattttac agtctagtgc taaacatatc
    12121 ttctccattg tatccataga aacagattta tcatttccta gtctgcctaa aacagaaata
    12181 cttatacctg atatatttac aaattccatt ttctttattt ttctgtcaat taacaatttc
    12241 cataatggag cgtatgaaat catgttatca cctcgcaaaa tatattctat cacattttat
    12301 ctattttgga aaattttcat ctatttagat aaataattat ttatctaaat agatttcaat
    12361 tatagtttat taaatataaa tatgttaaaa taaaacaaaa gcataagttc aaaaattaaa
    12421 ctttatatat atttatctca ttattttact aaaacat
//
LOCUS       DAELLV010000103        12346 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.103, whole genome shotgun sequence.
ACCESSION   DAELLV010000103 DAELLV010000000
VERSION     DAELLV010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12346
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.103"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..278)
                     /gene="srtB"
                     /locus_tag="KPD58_002195"
     CDS             complement(<1..278)
                     /gene="srtB"
                     /locus_tag="KPD58_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtB"
                     /protein_id="HBG0265958.1"
                     /translation="MKKLYRIVINIILVLVILYSGFNIYSKLTKYNHDTKISSELQKK
                     EYKKEDLSNINSDFKFWLSVENTNINYPVVQSKDNSYYLDKDFYKKDS"
     gene            410..744
                     /gene="rnpB"
                     /locus_tag="KPD58_002196"
     ncRNA           410..744
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="KPD58_002196"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            1022..3565
                     /locus_tag="KPD58_002197"
     CDS             1022..3565
                     /locus_tag="KPD58_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HBG0265959.1"
                     /translation="MKVLSENNIIRNTVLNVTASFLKQESKINEKLDGVLEKKFEKVE
                     FNEAKYAELLKFNILFYKTLARNTEPLIGKWIVDKYIPEIDELEKDLELTTAKCRKYV
                     NKAMKEGLDSLKANDLNSFLAYDKMELSERRRRLEKDYKVLNLYKDLLNISLRKISLE
                     KKECGDLFLKNQADAKRELKREIIFCVNKILASNKDVVPNNVEENNEQDKVEIKATEI
                     EDLQLGKDNSENDMLLNEIKNSKVKHESKSNDIESKFIDKLNSLNNESVSQTIHNEYK
                     KLCGLEELHSVEGYGFGKEIIKDFACATVVLEFLKRRNRDLIEGAMRLTIIGEFGPEN
                     FKEFVDYVIKNKTEISEDTWNKAQNLIKDNYSELENHEIASKRTRRNKDIDIEEYIYM
                     IKNADKDICFRSSISIEDDAEEGVKEEIDSNNQDIGDVVEDKDTTDKEYDSNKEDIIE
                     PENKKSKKKAKLFGFIKKDNEEVEQEEENLNDISPDIILDKPVENHQVKSEEIEQNEL
                     KEIKQEEPSQHIEEERNVKIEKPINNNLDEKVSSNNESKLEKESKNLEDKKAKEIKEE
                     KLENEKSVVIPIKKKENSNKKSKNSSKDKYRENKKEMKNYVSDKEDSLDDEEVVSKKS
                     RLKETIIAVVIVVIVGVGYFITVGNNKKNDKENIPKSSTQQQANNKLTEEEKKAQAEK
                     EKKEAEEKAKAEKEAQEKAQAEKKAKEMEAYKDGKGVYYTVYAGSLKVEKTAKETAKE
                     YEAKGISSTIIQENGYYKIKIGDYSQYGEAQEKCNELAKKSIDTYIAMYDKYYDYKLE
                     ELKESAPSLSAEELKQKYEDLRSELKNKSGYREYVKHLDKLYEEIVEGA"
     gene            3688..5679
                     /locus_tag="KPD58_002198"
     CDS             3688..5679
                     /locus_tag="KPD58_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265960.1"
                     /translation="MLKLGEKIIYELSDGFELSGISRYSKEYKKLHITNLGNIIISKS
                     DEVNKDTGVKYIYSFSDNKEKLEAVLEDSQIIIYGNNLPFYIKSENSSKNVTANLTMK
                     ITLDEYDLICKSQREFIFYLKDNMTILSLDDNKFYMGGINKDNEKFIFISGKNRFEIN
                     FDDIERYILEDKRVSLKGYFHMEREGIIVRSVSIFNNNIDSVVPSDLNERVKDNQKIG
                     NLPKDCEIVFCKISGNIDGFDYKNTNMLLVKYQDQLIFINKKSKKTIVKSAKDNCSKL
                     NLGEDIILYDNKNVFNLHINDKNREIMQIDDLKDIENEIVGYTLKHAPFFIQEDFDSL
                     TILKSFQKEIISIKNSDIKDIVINKELENENSNFVETEIKFNNQKVLLNLSKSMVQKL
                     MQDVFIYAKQPLLKENSIEVIYKNWSKAMNDMIIFNFFGNIYYMKSEFDKILEKELND
                     EIRIEVINSLYKQIQEQRNNLDLLSAYMPRILENQEIDLFEKYNTKLDVQVFKQIKNL
                     LSDLSYNISSYLNEVEKSLDNIIFVISGEDKKKYNYRMLKESESASLDVFLKQAISRL
                     NHLVENMYPYYVDETSREMFKLFELLWKNYRNIDDDSIKEILFERITNTYVFKQLTLN
                     NSTKERRKDIIEKIYNSVDYGTNKLDENMFFTGGIKYVK"
     gene            5669..5989
                     /locus_tag="KPD58_002199"
     CDS             5669..5989
                     /locus_tag="KPD58_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0265961.1"
                     /translation="MSSKYNVWTFNYEFLGLDSGDEKNNEVYYEIDLDEILNENYIDE
                     DIDSGLDSDGNSILGELISEMDIDEDVSVDMTYQELEVDLEDLDSYLDEDIDSDIKGI
                     LDEI"
     gene            6187..7056
                     /gene="galU"
                     /locus_tag="KPD58_002200"
     CDS             6187..7056
                     /gene="galU"
                     /locus_tag="KPD58_002200"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBG0265962.1"
                     /translation="MTVKKAIIPAAGLGTRFLPATKSQPKEMLPIVDKPTLQYIIEEA
                     IESGIEEILIITGRNKKSIEDHFDKSVELELELEQKGKTEMLEMVRDISNMVNIHYIR
                     QKEPKGLGHAIYCAKSFIGDEPFAVLLGDDIVDSDVPCLKQLIDTYNEYKTTVLGVQK
                     IAKEDTNKYGILDVKHIEDRVYKVKDMVEKPAIEEAPSDIAILGRYIITPAIFSILEK
                     QTPGKGGEIQLTDALQTLGKQEAIYAYNFEGRRYDVGDKIGFLEATIDFALKRENLKD
                     DLMDYMRKKVSEK"
     gene            7080..8093
                     /gene="galE"
                     /locus_tag="KPD58_002201"
     CDS             7080..8093
                     /gene="galE"
                     /locus_tag="KPD58_002201"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBG0265963.1"
                     /translation="MAVLVAGGAGYIGSHTAIELLESGYEVVIVDNLSNSNSIVVDRI
                     KELSKKPVKFYNIDIRNKDEMHVVFKENNIESIIHFAALKAVGESVEKPIEYYSNNLI
                     STLNLFELMREYGVKKFVFSSSATVYGDPHTCPILEDFPLSVTNPYGRTKLMIEQMLV
                     DISKADKSLDIALLRYFNPVGAHKSGRIGEEPNGVPSNLMPYITKIAVGKLKELSVYG
                     NDYPTHDGTGVRDYIHVLDLAAGHVKALQKLEENPGLVVYNLGTGKGYSVLDLVKAFS
                     KASGKEIPYKIVGRRAGDVAMCYADSSKAEKELGWKAKYELEEMCEDSWRWQSMNPNG
                     YEE"
     gene            8205..10139
                     /locus_tag="KPD58_002202"
     CDS             8205..10139
                     /locus_tag="KPD58_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp22"
                     /protein_id="HBG0265964.1"
                     /translation="MKKSLKKYLVLALTLVLVFACGVPESSAASKHVIIVNSRKNTLG
                     YFVNNKLVKEFRVATGKKGSETPTGKTKVVNKIKNRPYYKGNIPGGSPRNPLGDRWMG
                     LALKGTYGDTYGIHGNNNESSIGKHISGGCIRMHNKDVRWLFDQVPVGSDVIIDYSND
                     SYVKIAAKYKINLNQTGWKTENGKKYYVKSDGTYQKNSWLKVNGKMYYFDASGVMQTG
                     WKTINNKKYYLGTDGARVSGWKVIDGKTYYFNSDGVMQTGWQEKNGKKYYLGSDGLAV
                     TGWQEIDGNKYYFDKTGIMQTGWQQIDGKSYYLDKDGKMLTGSQKIDGKDYTFNEDGT
                     INPTWDTIIGANRFDTAKKISSVGNWNADSSDTVILVNGNAIADGITATPLASSYDST
                     ILLTNTANLPTETVEEMKLLAPKTVILIGGENAISSKLEQEIKTTFNTETKRIAGQDR
                     YQTATRIAEELGNREEIKTAYMVSGNGEADALSVASKAGEEKQPIILVNKDGITEESY
                     KWLTERKLENAYFIGGPSAINDSVIAKMNDITTEDISGNRIYGDSRVDTNAKVIEKFY
                     GDTDLQAVLVSKSDALVDALSAGPLAVKLHSPIVLMDNSGLSSEQQRVFANKKVETPY
                     QIGGGVSYIVMDKLMDILAK"
     gene            10294..11157
                     /locus_tag="KPD58_002203"
     CDS             10294..11157
                     /locus_tag="KPD58_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HBG0265965.1"
                     /translation="MLSSYVKRVSKIIKKKYIMFIDESGRADINHEDPFTLTGVIFEY
                     KYAVSQGDYVCSLRKEMDAFKEYCFGTSNIGIHLTDISRKSRAFEKFDDSQIKLFYDE
                     LPLFLSRLECTIISVTVDKARLKKYYAPSKEPYVVAFIHVLQNFYSFVNNETVESARI
                     VIESRDDVSNLKVQKAFFDVFNNGTIYLDIDEELRDKVKGFIIAKKEDADYREGLEIA
                     DILCNPLSRARRGLIEANPKCMQYGEKNKIFKSVRNKIYTPTSVNDIRNWGFKEVPIV
                     EPVGPWLEKMR"
     gene            complement(11365..12246)
                     /locus_tag="KPD58_002204"
     CDS             complement(11365..12246)
                     /locus_tag="KPD58_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG0265966.1"
                     /translation="MNLYHLRYFVTLAHLEHYTKAAENLSITQPSLSHAISLLENELG
                     VALFEKEGRNIVLTKYGKIFLKDVEKSLEILDSSVKSLKITGTGEGQIDLAFLRTLGT
                     DFIPDIVHKFLKSNPAKSIDFKFHTGVTTDIIQGLKERKYDIAFCSKLEKEKGIEFIP
                     VAKQDLVLIVPYSHPLAAKDTIDLKETIPYPQIVFNQRSGLRYIIDDMFKKINQQPNI
                     VYEVEEDQVIAGLVAKNFGIAVVPNMNMLSFTKVKVIQIIHPSWERNFYLAFIKDRYL
                     PPAIKNFKNFVIKNAQL"
BASE COUNT         5180 a         1245 c         2195 g         3726 t
ORIGIN      
        1 atagaatctt ttttataaaa atctttgtca agatagtaag aattatcttt agattgtaca
       61 actggataat ttatatttgt attctcaaca gaaagccaaa atttaaaatc actgttaata
      121 tttgataaat cctctttttt atattctttt ttttgaagtt ctgaggatat tttagtatca
      181 tgattgtatt tggtaagttt tgagtatata ttaaatccac tgtaaagtat tactaatact
      241 agtataatgt taattactat tctatataat tttttcaata tatcacctcg agttaaatgt
      301 agtttatata tctatattac actatttttg aataaattta aatttttagt ataatattac
      361 tttgacatgt aaaagaattt gtgttaacat aactaagtaa aataaatatt aagtaagcca
      421 gataatcgca tgtaatctta catgaggaaa gtcggagctc catagagcag ggtgctaggt
      481 aacgcctagt gggagcgatc ctaaggatag tgcaacagaa ataaaccgcc actattgtgg
      541 taaggatgga aaggcgaggt aagagctcac cagcagctag gcgactagtt ggctatgtaa
      601 accccacctg gagcaagacc aagataggaa taaagagtgc tgctcgtact ctccggtagg
      661 taggtcgctt gagcctattg gtgacaatag gcctagaaag atgattatct aatacagaac
      721 tccgcttata gacttgctta attatttcga tagtcaaaaa catcagttta aactgatgtt
      781 tttttatttg taaatttgtc taatctactt ttaaaatttt gaggaatatt gtaatataag
      841 ttagtacaga tattaaaaat ggtataatat aagatagata taaaaagaaa aagtaaaatg
      901 atgtaaataa tatgatatga tataagatgt agcaaattta gtaaataatt atttagatat
      961 cataatcaaa ttagcaactt tgcacaatgt aaatattaca ataaaataat tgatttaagg
     1021 agtgaaggtt ttgagtgaaa ataatataat aagaaacact gttttaaatg tcacagcaag
     1081 ttttttaaaa caagagtcta aaataaatga aaagctagat ggtgttttgg aaaagaaatt
     1141 tgaaaaagta gaatttaatg aagcaaaata tgcagagctt ttaaaattta atatattgtt
     1201 ttataagaca ttagcaagaa atacagaacc acttattggc aaatggatag tagataagta
     1261 catacctgaa atagatgaat tagaaaaaga tttagaatta actacagcaa aatgtagaaa
     1321 gtatgtaaat aaggctatga aagagggatt agactctcta aaagccaatg atttaaatag
     1381 ttttttagct tatgataaaa tggaactatc agagagaaga agacgtttag aaaaggatta
     1441 taaggtttta aacctatata aagatcttct taatatatct cttagaaaaa taagtttaga
     1501 aaagaaagaa tgtggagatt tatttcttaa aaatcaagca gatgcgaaaa gagaattaaa
     1561 aagagaaatt atattctgtg taaacaaaat attagctagc aataaagatg tggtacctaa
     1621 taatgtggaa gagaataacg aacaagacaa agtagaaata aaagcaactg aaattgagga
     1681 tttgcaatta ggtaaagata attcagaaaa tgacatgcta ttaaatgaaa ttaaaaattc
     1741 aaaagtaaaa catgaatcaa aatcaaatga tatagaatcg aagtttatag ataagttaaa
     1801 tagcttaaat aatgaaagtg tatctcaaac aatacacaat gaatataaga agttatgtgg
     1861 attagaagaa cttcattctg ttgaaggata tggttttggc aaagaaatta ttaaagattt
     1921 tgcttgtgca acggttgtat tagaattttt aaagagaaga aatagagacc ttatagaagg
     1981 tgcaatgaga ctgactataa ttggagaatt tggtcctgaa aattttaagg aatttgtgga
     2041 ctatgtaatt aaaaataaaa cagaaattag tgaagataca tggaataaag cacagaatct
     2101 aataaaagat aactatagtg aattagaaaa tcatgaaatt gcatcaaaaa gaactagaag
     2161 aaataaagat atcgatattg aagaatacat ttacatgata aaaaatgcag ataaagatat
     2221 ttgctttaga agtagtattt caatagaaga tgatgctgaa gaaggggtta aagaagaaat
     2281 agatagtaat aatcaagata taggtgacgt tgtagaagat aaagatacta cagataaaga
     2341 gtacgacagc aataaagaag atataattga accagaaaat aaaaagagta aaaagaaggc
     2401 taaattattt ggatttatta aaaaagacaa tgaagaagtt gaacaagaag aagagaattt
     2461 aaatgatatt tctcctgata taattttaga taaaccagtt gaaaatcatc aagtaaaatc
     2521 agaagaaata gagcaaaatg aattaaaaga aataaaacaa gaagaaccaa gtcaacatat
     2581 agaagaagaa cgtaatgtaa aaatagaaaa accaataaat aataacttag atgaaaaagt
     2641 atcttcaaat aatgaatcga agcttgaaaa agaaagtaaa aacttagaag ataaaaaagc
     2701 aaaagaaata aaagaagaaa aattagaaaa tgaaaaatca gtagtaatac ctattaaaaa
     2761 gaaagaaaat agtaataaaa aatctaaaaa ttcttctaag gacaaatata gagaaaacaa
     2821 gaaagaaatg aaaaattatg tatcagataa agaagattct ttagatgatg aagaggttgt
     2881 ttctaagaag agtagattaa aagaaactat tatagcagta gttatagtgg ttattgttgg
     2941 tgtaggatat tttataacag taggcaacaa taagaaaaat gataaagaga atattccaaa
     3001 gtcatcaact caacaacaag ctaacaataa attaactgaa gaagagaaaa aagcacaagc
     3061 tgaaaaagag aaaaaagaag cagaagaaaa ggcaaaagca gaaaaagagg ctcaagaaaa
     3121 agcacaagct gaaaagaaag caaaagaaat ggaagcatat aaagatggta aaggtgtata
     3181 ttacactgtt tatgcaggtt cacttaaagt tgaaaaaaca gcaaaagaaa cagcaaaaga
     3241 atatgaggct aaaggtattt cttcaacaat aattcaagaa aatggatact ataaaataaa
     3301 aataggtgat tattctcaat atggagaagc acaagaaaaa tgtaatgaac tagctaagaa
     3361 atctattgat acatatatag ctatgtatga taagtactat gattataagt tagaagaatt
     3421 aaaagaatca gctccttctt tgagtgctga agaacttaaa caaaaatatg aagatttaag
     3481 aagtgagtta aagaacaaat ctggatatcg tgaatatgtt aaacatttag ataaacttta
     3541 tgaagaaata gttgaaggtg cttagagcat tatagaaaag gagaaactat gtctcagaat
     3601 aaaaagtatt aaaaaaaata ttatttattg tgagacattt tgttcacctt tataaaatac
     3661 ttataattct gtttaggtgg tgaaggtatg ttgaaattag gagaaaaaat catatatgag
     3721 ctttcagatg ggtttgagct tagtggcata agtagatact caaaagaata taaaaagctt
     3781 catataacaa atctagggaa tataataatc agcaaatcag atgaagttaa taaggataca
     3841 ggtgtaaaat atatatatag tttttcagac aataaagaaa aattagaggc agttttagaa
     3901 gattctcaaa taattatcta tggaaataac ttgccttttt acataaaaag tgaaaattca
     3961 agtaaaaatg ttacagcaaa tttgactatg aaaataactt tggatgaata tgatttaatc
     4021 tgcaaaagtc aaagagaatt tatattctat ctaaaggata atatgactat attaagttta
     4081 gatgacaata agttttatat gggtggaatt aataaagata atgagaagtt tatatttatc
     4141 agtgggaaaa atagatttga aataaatttt gatgatatag aaagatacat tttagaagat
     4201 aaaagagtgt cattgaaggg atactttcat atggaaagag aggggattat agtaaggtct
     4261 gtatctatat ttaataacaa tatagatagt gtagtcccat ctgatttaaa tgagagagtt
     4321 aaggataacc aaaaaatagg aaatcttcct aaagattgtg aaatagtatt ctgcaagata
     4381 tcaggaaata tagatggatt tgattataaa aacacaaaca tgcttttagt aaaatatcaa
     4441 gaccaattaa tttttataaa taagaaatct aagaagacta ttgtaaagtc agcgaaagat
     4501 aattgttcta agttaaattt aggagaagat ataatattat atgataataa aaatgtattt
     4561 aacctacata taaatgataa aaatagagaa attatgcaaa tagatgacct aaaagatata
     4621 gaaaatgaaa tagtgggata tacattaaaa catgctccat tttttattca agaagatttc
     4681 gatagcttga ctattctaaa gtcttttcaa aaagaaatca tatctataaa aaatagtgat
     4741 ataaaagaca ttgtaattaa taaagaactt gaaaacgaaa attctaattt tgtagaaacg
     4801 gaaataaaat ttaataatca aaaagtactt ttaaacttaa gtaaaagtat ggttcaaaaa
     4861 cttatgcaag atgtatttat atatgcaaag caacctctac ttaaagaaaa ttctattgaa
     4921 gtgatatata agaattggtc taaagctatg aatgatatga taatatttaa tttctttgga
     4981 aatatttact atatgaaatc tgaatttgat aagattttag aaaaagagtt aaatgatgaa
     5041 ataagaatag aagttataaa ttcactttat aagcaaatac aagaacaaag aaacaattta
     5101 gatttattgt cagcatatat gccaagaata ttagaaaatc aagaaataga tttgtttgaa
     5161 aagtataata ctaaacttga tgtacaagtg tttaagcaga ttaaaaatct attgtctgat
     5221 ttatcatata atataagcag ttatttaaat gaagttgaaa aaagtttaga caatatcatc
     5281 tttgtaattt caggagaaga taagaagaaa tacaattata gaatgttaaa agaaagtgaa
     5341 agtgcaagtc ttgatgtatt tttaaaacaa gccatatcaa gattaaacca tttagtagaa
     5401 aatatgtatc cttattatgt agatgaaact agtagggaaa tgtttaagtt atttgagtta
     5461 ttgtggaaaa actatagaaa tattgatgat gatagtataa aagaaatatt attcgagaga
     5521 ataacaaata catatgtatt taaacaattg acattaaata atagtacaaa agaaagaaga
     5581 aaagacataa ttgaaaaaat atataactct gttgattatg gaaccaataa actagatgaa
     5641 aatatgtttt tcacaggagg aataaagtat gtcaagtaaa tacaatgttt ggacttttaa
     5701 ctatgagttc ttaggattgg actcagggga tgaaaaaaat aatgaagttt attacgagat
     5761 tgatttagat gaaattttaa atgaaaacta tatagatgaa gatatagata gtggtttaga
     5821 ttctgatgga aatagtattt taggagaatt aatatcagaa atggatatag atgaggatgt
     5881 cagtgttgat atgacatatc aagaactaga agttgactta gaagatttag attcttatct
     5941 agatgaagat atagatagtg atataaaagg catcttagat gaaatttagt acctgtataa
     6001 gttttggata aaatataaac aatacaatat taacttataa aactaaaggt agatgaaatt
     6061 tcatctacct tatattatct tatagcatta aatattggga taatatagag tttgaagaaa
     6121 aagtataaat atggtatatt aatttggtac aatagaaaat ttacgaagaa gggtgaaaaa
     6181 atgcagatga cagttaaaaa agcaataata ccagcagcag ggctaggaac tagattttta
     6241 ccagcaacaa aatcacagcc caaagaaatg cttcctatag tagataaacc aactttacag
     6301 tatataatag aagaagctat agaatcggga attgaagaaa ttcttataat aacaggtaga
     6361 aataaaaaat ctatagaaga tcattttgat aaatcagttg aattagagtt agaactagag
     6421 caaaaaggta agacagagat gttagaaatg gtaagagaca tatcaaatat ggtaaatatt
     6481 cattatataa gacaaaaaga gccaaaaggt cttggacatg caatttactg tgcaaaaagt
     6541 tttataggtg atgaaccttt tgccgtatta ttaggtgatg atatagtaga ttcagatgtt
     6601 ccgtgcttaa aacaattaat agatacttat aatgagtata aaacaactgt attaggtgtt
     6661 caaaagatag ctaaagaaga tactaataaa tatggtatat tggatgtaaa acatatcgaa
     6721 gatagagtat ataaagttaa agatatggtt gaaaaacctg caattgaaga agctccatct
     6781 gatatagcta tacttggaag atatataata actcctgcaa tatttagcat attagagaaa
     6841 cagacacctg gtaaaggtgg agagatacaa ctgacagatg cacttcaaac tcttggaaaa
     6901 caagaggcta tttatgctta taactttgaa ggtagaagat atgatgtggg agataagatt
     6961 ggttttttag aggcaacaat agactttgct ctaaagagag aaaatttaaa agatgattta
     7021 atggactata tgagaaaaaa agtaagtgaa aaataaatat tagaaaagag gtaattacaa
     7081 tggcagtttt agtagctgga ggagcaggat atataggaag ccatacagca atagaacttt
     7141 tagaatcagg ttatgaagtt gttatagttg ataacttaag taatagcaac tcaatagtgg
     7201 ttgatagaat caaggaatta tctaaaaaac cagtaaaatt ttataatatt gatatcagaa
     7261 ataaagatga aatgcatgtt gtatttaaag aaaataatat tgaatcaatt atacactttg
     7321 cagctttaaa agcagttggt gaatcagtag aaaaaccaat tgaatattac agcaataatc
     7381 taataagtac cttaaattta tttgaattaa tgagagaata tggtgttaaa aagtttgtat
     7441 ttagttcttc agctactgta tatggagacc cacatacttg tcctatatta gaggattttc
     7501 cactttctgt aaccaatcca tatggaagaa ctaaacttat gatagagcag atgttagtag
     7561 atatatcaaa agcagataag tcattggata tagctctttt aagatacttt aatccagtcg
     7621 gtgctcataa aagtggtaga ataggtgaag aaccaaatgg agttccgagc aatctaatgc
     7681 cttatataac aaaaatagca gtaggtaaat tgaaggaatt gagtgtttat ggtaatgact
     7741 atccaacaca tgatggaact ggagttagag actatataca tgtattagat ttagctgctg
     7801 gtcatgtaaa agcattacaa aaattagaag aaaatccagg acttgttgtt tataatttag
     7861 gaactggaaa ggggtatagt gttcttgatt tagtaaaagc attttctaaa gcaagtggaa
     7921 aagaaatacc atataagatt gtaggaagaa gggctggaga tgtagccatg tgttatgcag
     7981 attcttcaaa ggcagaaaaa gagcttggat ggaaagctaa gtatgaactt gaagagatgt
     8041 gtgaagattc atggagatgg caaagtatga atcctaatgg gtatgaagaa tagaatatat
     8101 acttatatgt ataaatgtaa caaatttgta aatataaggt attggtaatt ttatgttata
     8161 attagacaca tgtagtaatg tttctaaaag agggggaagt tatagtgaaa aaaagtctaa
     8221 aaaagtattt agtactagca ctgactttag ttttagtatt tgcatgtggc gtaccagaat
     8281 caagcgcagc cagtaaacat gtgattatag taaattcaag aaaaaatact ctaggatatt
     8341 ttgtaaataa taaacttgta aaagaattta gagttgcaac aggtaaaaag gggtcagaaa
     8401 caccaacagg gaaaacaaaa gtagttaaca agataaaaaa tagaccatat tataaaggta
     8461 atatacctgg aggaagtcca agaaatccat taggagatag atggatggga ttagcattaa
     8521 aaggaactta tggagataca tatggaatac atggaaataa taatgagagt tctataggaa
     8581 agcatatttc tggtggatgt ataagaatgc acaataaaga tgttagatgg ttatttgacc
     8641 aagttcctgt aggttcagat gtaattatag attatagtaa tgacagctat gtaaagatag
     8701 cggcaaaata taagataaat ctaaatcaaa caggatggaa aacagaaaat ggtaagaagt
     8761 attatgtaaa atctgatgga acataccaaa agaatagttg gctaaaagta aatggaaaaa
     8821 tgtattactt tgatgcttca ggagtaatgc aaacaggatg gaaaacaatt aataacaaaa
     8881 aatattattt aggaactgat ggagcaagag tatctggatg gaaagtaata gacggaaaaa
     8941 cttattactt taactcagat ggagtaatgc aaacaggatg gcaagaaaag aatggaaaga
     9001 aatactatct aggatctgat ggtttagcag taacaggatg gcaagaaata gatggtaata
     9061 agtattactt tgacaaaaca ggaataatgc agacaggatg gcaacaaata gatggtaagt
     9121 cttactattt agacaaagat ggaaagatgc taactggttc tcaaaaaata gatgggaaag
     9181 attatacttt taatgaagat ggaactataa atcctacatg ggatacaatt ataggtgcaa
     9241 atagatttga tacagctaaa aagataagtt cagttggaaa ctggaatgct gatagctcag
     9301 atactgtaat attagtaaat ggtaatgcaa tagctgatgg tataacagca actcctttag
     9361 caagtagcta tgactcaaca atactactta ctaatacagc taatttacca actgaaactg
     9421 ttgaagaaat gaagttatta gcacctaaaa cagtaatctt gataggtgga gaaaatgcaa
     9481 taagttctaa attagagcaa gaaataaaaa ctactttcaa cacagaaaca aagagaatag
     9541 caggtcaaga tagatatcaa actgctacaa gaatagctga agaacttgga aatcgagaag
     9601 aaataaaaac tgcatatatg gtttctggaa atggggaggc agatgcttta tctgtagctt
     9661 ctaaagctgg ggaagaaaaa caacctataa tattagtcaa taaagatgga attacagaag
     9721 aatcttataa atggctaaca gaaagaaaat tagaaaatgc atactttata ggtggaccaa
     9781 gtgcaattaa cgattcagta atagcaaaaa tgaatgatat aactactgaa gatatttcag
     9841 gaaatagaat atatggagat agtagagtag atactaatgc caaagtaata gaaaaattct
     9901 atggagatac tgatttacag gctgtattag ttagtaaatc agatgcttta gtagatgctt
     9961 tatcagctgg accactagct gtaaaattac attcaccaat agttttaatg gataattcag
    10021 gattatcatc agagcaacaa agagtatttg caaataaaaa agttgaaact ccttatcaaa
    10081 taggaggcgg agtatcttat atagtaatgg ataaattaat ggatattctt gcaaaatagt
    10141 aaaaatatat attgaaaaat aattataatt gagatgtgaa attcaatcaa taaaagcttg
    10201 attgttttac atctcaattt tttagtataa aattaagttt aagttatata aaaatcttgg
    10261 aatattttta aaactcatat aaaatgaata tagatgctat catcatatgt taagagggtg
    10321 agtaagatta taaagaaaaa atatataatg tttattgatg aaagtggaag agctgatata
    10381 aatcatgaag acccatttac attgacagga gtaatttttg aatataaata tgcagtaagt
    10441 caaggcgatt atgtatgttc tttgaggaaa gaaatggatg catttaaaga gtattgtttt
    10501 ggaactagta acataggaat acatttgaca gatatatcaa gaaaatcaag agcatttgag
    10561 aagtttgatg attcacagat taagttattc tatgatgagt tgcctttatt tctatctaga
    10621 ctagagtgta ctataatatc agtaacagta gataaagcta gattaaagaa atattatgca
    10681 ccttcaaagg aaccgtatgt tgtggctttt atacatgttc ttcagaattt ttattcattt
    10741 gtaaataatg aaactgtaga atctgcacga atagttatag agtcaaggga tgatgtctca
    10801 aacttaaaag tacaaaaggc gttttttgat gtatttaata atggaacaat atacttagat
    10861 atagatgaag aattaagaga taaagttaag ggttttataa ttgccaaaaa agaagatgct
    10921 gactatagag aaggtttaga aatagcagat attttatgta atcctcttag cagagcaaga
    10981 agagggttaa tagaagctaa tccaaagtgt atgcaatatg gagaaaaaaa caaaatattc
    11041 aaatctgtca gaaacaaaat atatactcca acgagtgtga atgatataag aaattgggga
    11101 tttaaagaag ttcctattgt agagcctgtt ggaccatggc ttgaaaaaat gcgttagata
    11161 caattgacaa attttaaaaa atctgatata atgtaagtaa cctagttatt caggaactga
    11221 atccagttgc tgttgtataa cgataattac tgagactcgg atgccgactg tcagttagga
    11281 atctatactt ttaaaagtat agattttttt attttataaa aagaataaaa gaggatattt
    11341 aaaaataacc cctttattaa taaattataa ttgagcgttt ttaatgacaa agtttttaaa
    11401 gtttttaatt gcaggaggca aatatctatc ttttataaat gctaagtaaa agtttctttc
    11461 ccaacttggg tgaataatct gaattacttt tacttttgta aagctaagca tattcatatt
    11521 tgggactaca gcaattccga aattttttgc aactaatcca gcaattactt ggtcttcctc
    11581 tacttcatag actatattag gctgttgatt tattttttta aacatatcat caattatata
    11641 tctaagaccg cttctctgat taaatactat ttgaggataa ggtatggttt cttttaaatc
    11701 tatagtatcc tttgcagcta gtggatgact atatggtaca attagtacta aatcttgttt
    11761 ggcaacaggt ataaattcaa ttcccttttc tttttcaagt ttcgaacaaa atgcaatatc
    11821 atattttctt tctttaagac cttgaataat gtcagtagtt acaccagtat gaaatttaaa
    11881 atctattgat ttagcaggat tagattttaa aaatttgtgc actatgtctg ggataaaatc
    11941 agtaccaagt gttctaagaa aagctaagtc tatttgtcct tcaccagtac cagttatttt
    12001 taatgatttt acacttgaat ctaaaatttc taatgatttt tccacatctt ttaaaaatat
    12061 ttttccatac ttagtaagga ctatatttct accttctttt tcaaatagag ccaccccaag
    12121 ttcattttct agtaaagaaa tagcatgact taagcttggt tgtgttatag ataaattttc
    12181 tgccgctttt gtatagtgtt ctaaatgtgc caatgtaaca aaatagcgta agtgatataa
    12241 attcaatttt tgttcatcct ttcataggta tttttattta tatatttgta gaagttaacc
    12301 tttaagatat ttttacattt ttattatata tgattataga gtaaga
//
LOCUS       DAELLV010000104        12312 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.104, whole genome shotgun sequence.
ACCESSION   DAELLV010000104 DAELLV010000000
VERSION     DAELLV010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12312
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.104"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            56..874
                     /gene="larE"
                     /locus_tag="KPD58_002205"
     CDS             56..874
                     /gene="larE"
                     /locus_tag="KPD58_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HBG0265967.1"
                     /translation="MEVNFVKEKEKLDKLKKMLLELGSVVVAYSGGVDSNFLLKVAKD
                     TLGENVIAVTIHAMMHSSREIEEAKQYTQNFGVKHIILNIENFDLKEFKENGVDRCYH
                     CKKYIFSKIKEVAKEHNIKYIVDGTNIDDLGDYRPGLKALSELGVISPLKDSSLKKEE
                     IRSLSKTLGLNTFNKPSFACLASRIPYGVEITDENLRIVEKSEEYLSNLGFSQFRVRM
                     HGDIARIEVGQEELGKFFENNNFNKVDTKLKIFGFKYVTLDMSGYKMGSMNLNV"
     gene            929..2077
                     /locus_tag="KPD58_002206"
     CDS             929..2077
                     /locus_tag="KPD58_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HBG0265968.1"
                     /translation="MKNYTLISPCFFGMEKMLAREITNLGYEIIKTEDGRITYKTDEF
                     GIAKSNMWLRCAERVHLKIAEFEAKSFDELFENTKRINWSRYIPYGAQFPISKASSIK
                     SKLYSTPDVQAIVKKAIVESLKKSYLEDGLLKEDKEKYPIFVFIHKDKVTISIDTTGD
                     ALHKRGYREKANKAPIRETLAAGLIYLTPWKAGRVLVDPMCGSGTILIEAAMIGINMA
                     PGLNREFISEKWRTLDKKIWWDVRKDAFNKIDNESKFKIYGYDIDEESIDIARENAEI
                     AGVDEYIEFNVGDATQFKSEDEFGFIITNPPYGERLEDKDSVKQLYKELGYAFRKLKN
                     WSYYLITSYEDFEYEFGQKADKKRKLYNGMLKTNFFQYPGPKPPRNNK"
     gene            2213..3601
                     /locus_tag="KPD58_002207"
     CDS             2213..3601
                     /locus_tag="KPD58_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG0265969.1"
                     /translation="MKVLKLITVVALMMLFVTGCNIKTESPEQLAKIPDYDDTKKVLY
                     DGIDQLLKPDSSMILPSNTKEVGKINKVDLNSDGIDELVVFEQKEDLSNNVSQVGFVT
                     LSYNGEKYVLGDHFLENGESIEYANFYDLDSDGYKEVILLVKSKDKTNMHIYKVVDNE
                     ITKIYDLDASWLQNKEDFNDMKVKIGYIDGDDKLDILMLHLNNKTNEMFASVANFDGK
                     MKIKDSVKFENVKNLSELYITLGNVASSVGNSSAAIKGIVLDIPILKDNNYITQILYM
                     KDGKINKAFSDYDKTITKSYYIPVDDIDKDKIIEIPIVAGSTGNNKNTYSSKSSATIS
                     WYRWNGKEGADSSLIFTSQIYYNYKYNFKLFIPNNLFDKILVEQEFVGEKAVFKFYYF
                     DYSDRKNLFNIVVESKNKLEDRKNTGTQNSIVLQESDEYTFLLVVNNANEMDKLDMTI
                     DALKEYFSLIYG"
     gene            3630..4328
                     /locus_tag="KPD58_002208"
     CDS             3630..4328
                     /locus_tag="KPD58_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0265970.1"
                     /translation="MKEKILILEDEIGIRSFVSINLKREGYEIVEAGTGREAIEKMTT
                     EKDITIALLDVMLPDISGIEVCKFIRENFDQVGIIMLTAKAQEDDKIEGFISGADDYI
                     IKPFSIKELLVRVSALLRRVAKDDSSVKSSEIVSPPFILDIDKRKLFKNGKEIELTPT
                     EFSIVKYLISNAKQSLSRDQILDEVWGTNYLYDFKIVDVNIRRIRNKIEDDPSKPKYI
                     QTIWGYGYCFRKEE"
     gene            4331..5764
                     /locus_tag="KPD58_002209"
     CDS             4331..5764
                     /locus_tag="KPD58_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0265971.1"
                     /translation="MLHFEGLRKKVIKNYFIIIIIMVTLFEGLFMFYIQNYYYDSVKQ
                     LLESEIKYADEYNAITMETTSFEKKVKNIFDKQPLTKNSEFGISIIDKDKNIILDQYG
                     FKSKEKANYEDVNNALKDIKTKNLTPYTYRIPDTGEHVMSISLPLKVNNIIEGVVRYT
                     VSLDAIDNAILKQATWLILAGIFILIIAILISLKFAETLIKPLRELKKFANELAVGNY
                     NIKLEKMKIVDDEIGDLAQTFEHMAHEIDKSEKLKEEFISSVSHELRTPLTSIKGWSE
                     TLGYESITREELDLGLGIIQDETERLIKLVEELLDFSRLSSDRIKLHVDIVDVEGLIV
                     GVVNQLKVKAAEKDISLLFEFENEFIENIQGDKNRLRQVLINLIQNSFKFTSQGGYIK
                     VVASQDEEITTISVEDNGSGIEKQNLNKVLDKFFQEDYNKAGSGLGLAISNEIVKLHG
                     GRMKIESEKNVGTKITFNIKNKFAKQA"
     gene            5786..6523
                     /gene="truA"
                     /locus_tag="KPD58_002210"
     CDS             5786..6523
                     /gene="truA"
                     /locus_tag="KPD58_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBG0265972.1"
                     /translation="MRNIKMIVAYDGSRYKGYQKLGDNNMTIQEKLENVLSKMTNETV
                     EIIGSGRTDMGAHARGQVVNFRTNCMDSLDKIQKYLYEYLPEDIVVKTVEEVDERFHS
                     RYNVKSKTYMYKIDNNKYHNPFIRKYATHVSKKLDLDRMRKASEYLVGEHDFTSFASS
                     KSKKKSNVREIYSINIKEDDNVIEIYVEGNGFLYNMVRIIVGALIDVGLKRRAPQDIK
                     YMLESKDRCQSSDTAPAKGLCLWKVTY"
     misc_binding    6634..6841
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            7094..8293
                     /locus_tag="KPD58_002211"
     CDS             7094..8293
                     /locus_tag="KPD58_002211"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HBG0265973.1"
                     /translation="MKEKVVLAYSGGLDTSIIIPWLKENYEDIDVIAVCGNVGQEDKM
                     EDVYEKALQSGASKAYVDDISEEFVTETIFKAVKAEAKYEGKYLLGTSLARPIIAKKL
                     VEVAHKEGAKYICHGCTGKGNDQVRFEATIAALDPTIKVIAPWRIWDIKSREDAIDYA
                     EKHNIKVTATKAKIYSVDANLWHVSTEGGDIEHLENEHKKDVYKQCVDPEDACDVAEY
                     VEVYFEKGVPKKVNGEELSPVALIHKLNELGCKHGIGVIDIVENRLVGMKSRGIYETP
                     GGTILYEAHNILESATLDKDTLHFKQMVSYKYGELIYNGLWYCKLRESIDAFMEQTQD
                     NVTGTVKVKLYKGNIKPAGIFTENALYDEGISSFGNSELYDHKDAEGFINLFTLPLKI
                     RAMKAGK"
     gene            8540..9586
                     /locus_tag="KPD58_002212"
     CDS             8540..9586
                     /locus_tag="KPD58_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4872 domain-containing protein"
                     /protein_id="HBG0265974.1"
                     /translation="MRKIIEIKHHSHDYECMWNGIEDLYINKTGEYLPNDFFFLLSGF
                     GSFCYMKTNKADLKRMVALGDGRTKQMYKFLAPIVGFEYKHHEFKKYEQALKKAKSEI
                     DDGYPVILGALDMYYLPYYKKLYHKEHIPFHYILMVGYDDEGIYLYDCGRTELLHLSY
                     DKLRESMNCNSPGLSKENTICTIRMNTSKNKYNIAREAIAIKRDMFLNPPKGFLGYKG
                     FEKFIKELPQWNTELDKDDYDKILTNMVMFFGTVPTIPNAIKGIKEPDTVQFKGGFDK
                     MSRMLNSIGEEYKNNSFIQVADIFDKTAIIIEKISNIIIDYLTQTCDDTEQLPLLFSE
                     VLEHMKTGYLILDV"
     gene            9694..10857
                     /locus_tag="KPD58_002213"
     CDS             9694..10857
                     /locus_tag="KPD58_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0265975.1"
                     /translation="MRTVKQVSDLTGISVRALHYYDEIGLLKPNKITDAGYRLYDDES
                     IKTLQQILFFKEIDIPLREVKEIMSSQYFDKVEALKNQKKLLILKRKRLDELIELINQ
                     TLRGEGNIDFKEFDMSEYFNVLEEFKREHRNKVIKIYGSVEKYNEYIERVKSNEEKIA
                     KMAIKQYGTIEKFAKAIKTNFSSDILNLGEKFDRYKNDCLKEKHPKLKELYRKLVEDL
                     SRNPSSTDLQEIAKAITDISKKDYEIFSMDTGDDNWYYMVQNYLVNPMWIEEVDKKYG
                     SGAGKFIGQVLKTYLRDRKPKINTLYEKLVEDLSRDCSSREVQSIVEEIDNEMKRSNE
                     FYKIDNGERYFDYMSELYLQDSNYIKVTDKNYGDGASKFIGEAFKIYFDNNNC"
     gene            complement(10946..11326)
                     /locus_tag="KPD58_002214"
     CDS             complement(10946..11326)
                     /locus_tag="KPD58_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HBG0265976.1"
                     /translation="MKSTTFVILWTILFVLFGFYVNNKLYDFTEGYKDNISVLEKSIE
                     NEDWEKAQKEADSISTSWNKEKNHWYKVLNHEYFDEIGLRFNILDKAIYTENKLKSLE
                     EVESIKTYLGNIIESVKFDVNYIF"
     gene            complement(11329..11988)
                     /locus_tag="KPD58_002215"
     CDS             complement(11329..11988)
                     /locus_tag="KPD58_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBG0265977.1"
                     /translation="MTVVLIRSIILYITVLIALRVMGKGEIAEMNCFDLVITLLIAEV
                     ASVPMENNNIPIINGVAAISGLVIMQTLISFLSLKSRKLSSFLSGKPSVLIDKGKIVY
                     KELKKERISIDELLEQLRIQGYFNLKDVQYAILETDGNLSVVPASSYNSTPPRAFNHL
                     PIPLILDGRIINKNLDIAQKDTNWLMGILKSNHIETFKDVLICVLDENDKIFIQNKKG
                     D"
BASE COUNT         4944 a         1238 c         2127 g         4003 t
ORIGIN      
        1 tcattaagaa tgtaaaaaaa taatatatag ttttatagtt catatttttc cagatatgga
       61 ggtaaatttt gtgaaggaaa aagaaaaatt agataaatta aaaaaaatgt tacttgaatt
      121 aggcagtgtt gtagttgctt attctggtgg agtagatagt aattttttgc ttaaggtcgc
      181 aaaagataca ttaggtgaaa atgttatagc tgtgactatt catgcaatga tgcattcaag
      241 tagggaaatt gaagaggcta aacaatatac tcaaaatttt ggtgttaaac atatcatatt
      301 aaatatagaa aactttgatt taaaagaatt taaagaaaat ggtgttgata gatgttatca
      361 ttgtaaaaaa tatatattct ctaaaataaa agaggtagca aaagaacata atataaagta
      421 catagtagat ggaacaaata tagatgattt aggagactat agacctgggc ttaaggcatt
      481 gagtgaatta ggagtaatta gtccattaaa agatagtagt ttaaaaaaag aagaaattag
      541 gtcgctttca aaaacattag gattaaatac ttttaataaa ccatcatttg catgtcttgc
      601 gagtaggata ccttatggag ttgaaataac agatgaaaat ttaagaatag tagaaaagag
      661 tgaagaatat ctatccaatt taggtttttc tcaatttaga gtgagaatgc atggagatat
      721 agctagaata gaagttggac aggaagagct aggtaaattt tttgaaaata acaattttaa
      781 taaagttgat actaaattaa aaatatttgg ctttaaatat gttacactag atatgtcagg
      841 atataaaatg ggaagcatga atttaaatgt gtagattatt ttaaaaataa ataagtttta
      901 tagttaatat aaggaaacag gaggttttat gaaaaattat acgttaatat cgccatgttt
      961 ttttggaatg gaaaaaatgc tggctagaga gattactaat ttaggatatg aaataataaa
     1021 aacagaagat ggaagaataa catataaaac tgatgagttt ggaatagcaa agtcaaacat
     1081 gtggttgaga tgtgctgaaa gagtacatct taaaattgct gaatttgagg caaaaagctt
     1141 tgatgaatta tttgaaaata caaaaagaat aaactggtct agatatatac cttatggagc
     1201 tcaatttcct atatcaaaag cttcctctat aaaatcaaaa ttatacagta caccagatgt
     1261 acaagccata gtaaaaaagg ctatagtaga aagcttaaag aaaagttatt tggaggatgg
     1321 tttgcttaaa gaagataagg agaaataccc tatttttgta tttatacaca aagataaagt
     1381 aaccatttct atagatacaa caggagatgc tttgcataag agaggatata gagaaaaggc
     1441 aaataaagct ccaataagag agactttagc tgctgggctt atttatttaa ctccctggaa
     1501 agcaggtaga gttttagtag accctatgtg tggttctggt actatattga ttgaagctgc
     1561 tatgattggt ataaatatgg cacctggctt aaatagagag tttatatcag aaaagtggag
     1621 aactcttgat aagaaaatat ggtgggatgt aagaaaagat gcttttaata agatagataa
     1681 tgaatctaaa tttaagattt atggatatga tatagatgaa gaatctatag atatagctag
     1741 agaaaatgct gaaatagcag gtgtagatga atatatagag tttaatgtag gagatgcaac
     1801 tcaatttaaa agtgaagatg agtttggatt tataatcact aatcctcctt acggagaaag
     1861 attagaagat aaagacagtg ttaaacagtt gtataaggag cttggttatg catttagaaa
     1921 attaaaaaat tggtcgtatt atttgataac ttcttatgaa gactttgaat atgagtttgg
     1981 gcaaaaagca gataaaaaaa gaaaattgta taatggtatg ttaaaaacta actttttcca
     2041 atatccgggt cctaagcctc ctagaaataa taaataagtt ttacatgatt tatttgtaaa
     2101 atataaaatt ttattaaaat aagtaaaaca ttatgatgcc ctgaaacgtc taattattgt
     2161 gaggagattt ttgtgaacaa atttaaagat ttataggatt aggaggagtt aaatgaaagt
     2221 gttaaagctt attactgtag tagccctgat gatgttattt gttactggat gtaatataaa
     2281 aactgaatct ccagaacaat tagcgaagat acctgactat gatgatacaa aaaaagtatt
     2341 gtatgatggt attgaccagt tactaaaacc agattcatct atgatattgc cttctaatac
     2401 aaaagaagta ggaaaaataa ataaggtaga tttaaacagc gatggaattg atgaattagt
     2461 agtatttgaa caaaaagaag atttaagtaa taatgtgagt caggtagggt ttgtaacctt
     2521 gagttacaat ggagaaaaat acgtacttgg agaccatttt ttagaaaatg gcgaatcaat
     2581 agaatatgct aatttttatg atttggactc agatggatat aaggaagtta tcttattggt
     2641 taaatccaaa gacaaaacga atatgcatat atataaagta gtagataatg aaataacaaa
     2701 aatatatgac ttggatgcat catggcttca aaataaagaa gattttaatg acatgaaagt
     2761 aaaaattgga tatatagatg gagatgacaa gttagacata ttgatgcttc atttgaataa
     2821 taagactaat gaaatgtttg caagtgttgc aaattttgat gggaaaatga aaatcaaaga
     2881 ttctgttaag tttgaaaatg taaaaaactt aagtgaatta tatataacat taggtaatgt
     2941 tgcttcatct gttggaaatt cttctgcagc tataaaaggt attgtacttg atattcctat
     3001 attgaaagat aataactata ttacgcagat actttatatg aaagatggga aaattaacaa
     3061 agcttttagt gattatgata aaactattac taaatcttat tatatacctg tagatgatat
     3121 tgacaaagat aagattatag aaatacctat agttgcaggc agtacaggta ataataaaaa
     3181 tacttatagt tctaaatcat ctgcaactat aagttggtat agatggaatg gaaaagaagg
     3241 tgctgactca agtttaatat ttacaagtca gatatattat aattataaat ataattttaa
     3301 attatttatt ccaaataatt tatttgataa aatacttgtt gaacaggaat ttgttggaga
     3361 aaaagcagta tttaaatttt actattttga ctattcagac cgtaagaatt tatttaatat
     3421 agtagtagag agtaaaaata aacttgaaga tagaaaaaat acaggtactc aaaattctat
     3481 tgtacttcaa gagagcgatg aatatacttt cttactagtt gtaaacaatg caaatgaaat
     3541 ggataagcta gatatgacta tagacgcatt aaaagaatat ttctcattaa tatatggtta
     3601 atattatact caattggagg ttttatttta tgaaggaaaa gatacttata ctagaagatg
     3661 aaattggtat tagaagtttt gttagtataa acttaaaaag ggaaggctat gaaatagttg
     3721 aggcaggtac tggaagagaa gctatagaaa aaatgacaac agaaaaggat attacaatag
     3781 ctcttttaga tgtaatgctt ccagatataa gtggtataga agtatgtaaa tttataaggg
     3841 agaattttga ccaagtaggt ataataatgc ttactgctaa agctcaagaa gatgataaaa
     3901 tagaaggatt tatctcagga gcagatgatt acatcataaa accatttagt ataaaagagc
     3961 ttttagtcag agtttctgct cttcttagaa gagtagcaaa ggatgattca agtgttaagt
     4021 cgagtgagat agtttctcca ccatttatac ttgatataga caaaagaaaa ttatttaaaa
     4081 atggaaagga aattgagctt acaccaactg aattttcaat agtgaaatat ctaatatcaa
     4141 atgctaagca gtcgttaagt agagaccaga tattagacga agtatgggga actaactatt
     4201 tatatgattt taaaatagta gatgtaaata taagaaggat aagaaataaa atagaagatg
     4261 acccatcaaa accaaaatat atacaaacta tttggggtta tggatactgt tttagaaagg
     4321 aagaataata gtgttacatt ttgaaggtct gagaaaaaaa gtaataaaaa actattttat
     4381 aataattatt ataatggtta ctctgtttga aggattgttc atgttttaca tacaaaacta
     4441 ctattatgat tctgtcaaac agctacttga atctgaaata aaatatgctg atgaatataa
     4501 tgctattaca atggaaacta caagttttga gaaaaaagta aaaaatatat ttgataaaca
     4561 acccttaact aaaaattcag aatttggaat ttcaataata gataaagata aaaatattat
     4621 attagaccaa tatggtttta aaagtaaaga aaaagcaaac tatgaagatg ttaacaatgc
     4681 attaaaagat ataaaaacta aaaatttaac accttatact tatagaatac cagatacagg
     4741 ggagcatgtt atgagtatct ccttaccact taaggtaaat aatataatag aaggtgttgt
     4801 tagatataca gtatcattag atgctattga taatgcaata ttaaagcaag ctacatggct
     4861 tatattagca ggaatattta tacttattat agctatacta ataagtctta aatttgctga
     4921 gactcttata aaaccattac gagagctaaa gaaatttgca aatgaattgg ctgtaggaaa
     4981 ttataatata aaattagaaa agatgaagat tgtagatgat gaaattggtg atttagcaca
     5041 gacatttgaa cacatggcac atgagattga caaaagtgaa aaattaaaag aggaatttat
     5101 atcatctgta tctcatgaac taaggactcc tcttacatct ataaaggggt ggagtgaaac
     5161 cttaggttat gaaagtataa caagagaaga actagactta ggtttaggca ttatacaaga
     5221 tgaaacagaa agacttataa aactagttga agaattatta gacttttcaa ggctttcttc
     5281 agacaggata aaacttcatg ttgatatagt tgatgttgaa ggtttaattg taggtgttgt
     5341 aaatcaactt aaagttaaag cagctgaaaa agatatatca cttttatttg aatttgaaaa
     5401 tgaatttata gagaacattc aaggagataa aaatagatta agacaggttc tcataaattt
     5461 aatacaaaat tcatttaaat ttacgagtca aggtggttac ataaaagttg tagcttctca
     5521 agatgaagag ataacaacca tatcagtaga ggataatggt tcaggtatag aaaagcaaaa
     5581 tttaaataaa gtgttagata aatttttcca agaagattat aataaggctg gtagtggatt
     5641 aggactagca attagtaatg aaattgtaaa acttcatggt ggaagaatga aaatagaaag
     5701 tgaaaagaat gttggaacaa aaataacttt taatataaaa aataaatttg ctaaacaagc
     5761 ataaattctt agaaagagga ttaagatgag aaacataaaa atgatagtag catacgatgg
     5821 ttcaagatat aaaggatatc aaaaattagg tgacaataat atgactattc aagaaaaact
     5881 tgaaaatgtt ttaagtaaaa tgactaatga aacagtggag ataataggtt ctggaagaac
     5941 tgatatggga gctcatgcaa gaggacaagt ggtaaatttt agaactaatt gtatggattc
     6001 tttggataaa attcaaaaat atttatatga atatttacca gaagatatag ttgtaaaaac
     6061 tgttgaagaa gttgatgaaa gatttcattc aagatacaat gtaaaatcaa agacttatat
     6121 gtataaaatt gacaataata agtatcataa tccttttata agaaaatatg ctactcatgt
     6181 aagtaaaaaa ttagatttag atagaatgag aaaagcaagt gagtatttag taggagaaca
     6241 tgattttact agttttgctt cttctaaatc taagaaaaaa tcaaatgtaa gagaaattta
     6301 ttctataaat ataaaagaag atgataatgt tatagaaata tatgtagaag gaaatggatt
     6361 tttgtataac atggtaagga taatagtagg tgctttgata gatgttggtc taaaaagaag
     6421 agctccacaa gatattaagt atatgcttga atcaaaagat agatgtcaat catcagatac
     6481 tgcaccagct aaaggactat gtctttggaa agtaacatat taatcaatta tataaaactc
     6541 tctttaaaat aaaaataaaa taaattatat agttgacata aatggaaaaa taaactatta
     6601 tatattttat aagagagata taaaacttta tatttaaatc ctgtgagaaa gagagtaact
     6661 tctatgtgtt ttatagagag ctgaggatgg tggaagcttg gcaaataagt attagtgaaa
     6721 agaactttgg aggatactaa ttgaatttag tatgttagta ggtaatctcg ccttaagaga
     6781 aagagtggat aagtttatct tatcaattag agtggtaacg cggatatatt tcgtctcttg
     6841 gtttttccaa gaggcgtttt ttaatatatt taattattag tgtttatctg gtatgttgaa
     6901 catttactaa ttgattagct aagtttatca ataagagtgg taccgcggat atttcgcctc
     6961 ttagtatttt aaaatactaa ggggtttttt tagtattaaa atgattttat gtgagtctaa
     7021 attctcagag tcattcaata ttaagaattt ctataaaata aaaattataa tttaagattg
     7081 ggaggaatta aaaatgaagg aaaaagtagt attagcatat tcaggaggac ttgacacatc
     7141 aatcataata ccttggttaa aggaaaatta tgaagatata gatgttatag ctgtttgtgg
     7201 aaatgtaggt caggaagata aaatggaaga tgtatatgaa aaagctttac aaagtggagc
     7261 atcaaaggca tatgtggatg atataagtga agaatttgta actgaaacta tatttaaagc
     7321 cgtaaaagca gaggctaaat atgaaggaaa atacttatta ggaacttctt tagcaagacc
     7381 tataatagct aaaaaattag ttgaagttgc tcataaagaa ggtgcaaaat atatatgcca
     7441 tggatgtaca ggaaaaggta atgaccaagt tagatttgaa gctacaattg cagctcttga
     7501 cccaactata aaagttattg caccttggag aatttgggat ataaaatcaa gagaagatgc
     7561 tatagactat gctgaaaaac ataatataaa ggtaacagca actaaagcta aaatatattc
     7621 agtagatgcc aacctttggc atgtttcaac agaaggtgga gatatagagc acctagaaaa
     7681 tgaacataaa aaagatgttt ataaacaatg tgtagaccca gaagatgcat gtgatgtagc
     7741 tgaatatgta gaagtttact ttgaaaaagg tgtacctaaa aaggtaaatg gcgaagaatt
     7801 atctccagtt gcactgattc ataagttaaa tgagttgggt tgtaaacatg gaattggtgt
     7861 tatagatata gttgagaata gacttgttgg tatgaaatca agaggtatat atgagactcc
     7921 aggaggaaca atcctttatg aagcacataa tattttagaa agtgctactt tagacaaaga
     7981 tacattacat ttcaaacaaa tggtatctta taaatacgga gaacttattt acaatggact
     8041 atggtattgt aaattaagag aatctataga tgcatttatg gaacaaactc aagataatgt
     8101 tacaggaaca gtaaaagtta aactttacaa aggaaatata aaacctgctg gaatatttac
     8161 tgaaaatgct ctttatgatg aaggaatatc ttcttttgga aatagtgaac tttatgacca
     8221 caaagatgca gaaggattta ttaatttatt cactttacca ttaaaaatca gagctatgaa
     8281 agctggaaaa taataaaata aattatttaa gagtgtataa aaacgatttt taggttttta
     8341 tatgctcttt ttttatttga atttattaaa ttatacagta aatagtataa aaaaatatat
     8401 tatagttcca aatggcttaa taatgtgaaa atataagacc taaaatataa attttataaa
     8461 tacaatagtt attgactctc tcattgtaag agggtttata ctgaatctaa tgaaaattta
     8521 tagtgaggag atagattata tgagaaaaat aattgaaatt aaacatcatt cacatgatta
     8581 tgagtgtatg tggaatggta tagaagattt atatataaat aaaaccggtg aataccttcc
     8641 aaacgatttt ttctttttac tgtcaggttt tggttcattt tgttatatga aaacaaataa
     8701 agctgatttg aaaagaatgg tagctttggg agatggaagg acaaaacaga tgtataaatt
     8761 tttagcacca attgttggat ttgaatacaa acatcatgag tttaaaaagt atgagcaagc
     8821 attaaagaaa gcaaaatctg aaattgatga tggatatcca gttattttag gagctttaga
     8881 tatgtattat ctaccatatt ataagaagct atatcataaa gaacatattc cttttcatta
     8941 tatattaatg gtaggatatg atgatgaagg aatatatctg tatgattgtg gtagaacaga
     9001 attattacat ctttcttatg ataaattacg tgaaagcatg aattgcaatt cccctggatt
     9061 aagcaaagaa aatactattt gcacaataag aatgaatact tcaaagaata aatataacat
     9121 tgctagagaa gctattgcta taaagcgtga tatgttttta aatccaccta aaggattttt
     9181 gggatataaa ggttttgaaa agtttattaa agagttgcca cagtggaaca ctgaattgga
     9241 taaggatgat tatgataaga ttttgaccaa tatggttatg ttttttggaa cagtaccaac
     9301 aattccaaat gcaatcaaag gaattaaaga accagatact gttcaattta aaggtggatt
     9361 tgataaaatg agtagaatgc tcaacagtat tggggaggaa tacaaaaaca actcttttat
     9421 acaagtagct gatatttttg ataaaactgc tattataatt gagaaaatat ccaatattat
     9481 aatagattac ttaactcaaa catgtgatga tacagaacaa cttccgttat tgttctctga
     9541 ggtcttagag catatgaaaa caggatattt aatcttggat gtataaaaaa tatattgttg
     9601 accctaacat tacgtaatag tgtaatatga aaatatagct taaatatatt agtaggtatt
     9661 gatggctgta aaattaaaat ttgaggtgtg gatatgagaa cagtgaaaca agtttctgat
     9721 ttaactggaa taagtgtgag ggcattacat tattatgatg aaataggtct tttaaaacca
     9781 aataaaatta cagatgcagg atatagactt tatgatgatg aatctattaa aactcttcaa
     9841 caaattttat tttttaagga gattgatatt cctttaagag aggttaagga aataatgtca
     9901 agccaatatt ttgataaggt tgaagctcta aaaaatcaaa aaaaattact tattttaaaa
     9961 cgaaagagac tggatgaatt gatagagctt ataaatcaaa ctctaagagg agaaggaaat
    10021 attgatttta aagagtttga tatgagcgag tattttaatg ttttagaaga atttaaaaga
    10081 gagcatagga ataaggtaat taaaatttat ggtagtgtag aaaaatacaa tgaatatata
    10141 gaaagagtta agtctaatga agagaaaatt gctaaaatgg ctataaaaca atatggaact
    10201 attgagaagt ttgctaaagc tataaaaaca aattttagca gtgatatatt gaatttagga
    10261 gaaaaatttg atagatataa aaatgattgt ttaaaagaaa aacatccaaa attaaaagaa
    10321 ttatatagaa agcttgtaga agatttaagt aggaatcctt cttcaacaga ccttcaagaa
    10381 atagcaaaag cgataacaga tatatctaaa aaagattatg aaattttctc tatggatact
    10441 ggtgatgaca actggtatta tatggttcaa aattatctag taaatcctat gtggatagaa
    10501 gaagtagata aaaagtatgg aagtggagca ggaaagttta ttggacaagt actaaagact
    10561 tatctaagag atagaaaacc aaagataaat acattgtatg aaaaacttgt agaagatttg
    10621 agtagagatt gttcttcaag ggaagttcaa agcattgttg aagaaataga caatgaaatg
    10681 aaaagaagta atgaatttta taaaatagac aatggtgaaa gatactttga ttatatgtca
    10741 gagctttatt tacaagattc taactatata aaagtaactg ataagaatta tggtgatggt
    10801 gcatctaagt ttattggaga agcttttaag atttattttg ataataataa ttgttgaaaa
    10861 tttggaagta aaaaatatgt aagttttata aaagcacaaa aataaggatg tagattagct
    10921 ccatctatat ccttatttaa agttattaaa atatatagtt tacatcaaac ttaacacttt
    10981 caataatatt tcccaaatat gtttttatac tttcaacttc ttctaaagat tttaatttat
    11041 tttcagtata aattgctttg tccagaatat taaaccttaa tcctatttca tcaaagtact
    11101 catgatttaa aactttgtac caatggtttt tttctttatt ccaactcgta gatattgaat
    11161 cagcttcttt ttgagctttt tcccaatctt cattttctat agatttttct aaaacagata
    11221 tattgtcttt atatccttct gtaaaatcat atagtttatt gttaacgtaa aatccaaata
    11281 atacaaataa gatagtccat agtataacaa atgttgtaga tttcaatatt aatcaccctt
    11341 tttattttga ataaatattt tgtcattttc atctaaaaca caaataagaa catctttaaa
    11401 agtttctatg tgattagatt ttaaaatacc cattaaccag ttagtatctt tttgagctat
    11461 gtctagattt ttatttatta ttctaccatc cagtataagt gggataggta aatgattaaa
    11521 agctcttgga ggagtactat tgtagcttga tgctggtaca acacttagat taccatcagt
    11581 ctccaaaata gcatattgta catcttttag gttaaagtat ccttgaatcc taagttgttc
    11641 taataattca tctatactaa ttctttcttt tttaagctct ttatatacaa tttttccttt
    11701 atctattaat acagaaggtt tacctgataa aaatgaactt aattttctgc tttttaatga
    11761 taagaatgaa attagagtct gcatgattac aagtccagat attgctgcaa caccatttat
    11821 gattggtata ttattatttt ccattgggac agatgcaact tctgcaatta atagtgttat
    11881 aactaaatca aaacagttca tttcagctat ttcacctttt cccatgactc taagtgcaat
    11941 tagcactgtt atgtataata taatacttct tattaatact actgtcatat ctatcaatta
    12001 ctcctttaaa aaatatttac acttagttag tatatcctat ataaaaaatt taattcaaag
    12061 tataaaaaac cttaaataat tggattcaac tatttaaggt ttttttgata attttaatat
    12121 taaatttttt atggattttc tgttactttt atttatttta ataacagatt caacaaaact
    12181 ctttttatct cctaatttta agataaaatc aggatattct tcaaattcat tgagttttat
    12241 taatttgaaa gaagaagata gatttttaaa gttaataatt ttagagttat tcactaatac
    12301 acttcttttt tt
//
LOCUS       DAELLV010000105        12296 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.105, whole genome shotgun sequence.
ACCESSION   DAELLV010000105 DAELLV010000000
VERSION     DAELLV010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12296
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.105"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            53..928
                     /locus_tag="KPD58_002216"
     CDS             53..928
                     /locus_tag="KPD58_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HBG0265978.1"
                     /translation="MITFDVNGAIIRFDDTKNNYNKIRKIFKLYALEISKNFERDCLN
                     SFQNLKQISDRGLSLGEKYIEESFKKGIETIVSFGIITIDIDTFKSVYCEKYLDFKRL
                     FNNLNKTFIIPNKNKKNGHASFLEIESTIKKMSNYLYNDCFKIHYAVVDALIENRITA
                     VHSYIDEESIKKSNALFNNYKDGFIGKADECKVVKQIITLNPYREDVYEFLVKEDGDF
                     SKEIERLTEFLGYDIREYKASLMDIYINELMESDENDVENAKEKIEKYARYIGCSDEN
                     LYVARVNAIYTFANA"
     gene            1287..2585
                     /locus_tag="KPD58_002217"
     CDS             1287..2585
                     /locus_tag="KPD58_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase/BAAT N-terminal
                     domain-containing protein"
                     /protein_id="HBG0265979.1"
                     /translation="MKVNVYNVESFVDERLKIMVSDLIPNTKLKISVKMEFPWCKGEE
                     FSSYGVFCSNEKGEVDLDLVEPIEGTYKADNCMGLIYSLEKSKTEGKNFAENISIDKP
                     IIMNMIFETSIERKEVELKRIFKSEDITTKYISDEFIGKLFYKQNSNNKTILMLGGSD
                     GNLDALDLLAAPLASRGFNVLTVAYFALEGLPDKLEEVPLEYFEKVFEWISENEITST
                     KEIFVHGTSKGGELAFLLASRYEQIKKVVVSQPHIYCFQALNGLMSGNDTSSWSYKGE
                     PFPYIKVDNDIFFEEQKKNISKDIPFGFNSTYKKSLKRAENRKEARIKIENSKADILM
                     IAGKEDNIWNSYEACLEALEIFEENNYPYNVELLTYNNMGHPLPIPYIMPLKETLCMS
                     MSGGIFSSGGTVEGNSKGQFESWNRTIEFYKKPLSSDLNN"
     gene            2775..4166
                     /locus_tag="KPD58_002218"
     CDS             2775..4166
                     /locus_tag="KPD58_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HBG0265980.1"
                     /translation="MERLARREQPHIKFGMFKIRIPFIHYRFEKPEAIQGIISSMTSL
                     GTIGLSTQILGLPYEVAWSMAIINSILYCLHVFMGDPVVPGWITASITLTTAYLLKFS
                     MGIERIQALTALQIDLGIIFILMGITGVAGKLVSKIPNSIKGGILMGVGISTIISEFN
                     PKGRFDSYPISITVGILVACFVMFSERFDTLKVKNKFLFHLGEYGVVSAILVSLIVGI
                     FSREIEIPRFSFDNLVYIVDFKNLINTVSPFGIGFPSVMLFIQGIPMAFMIYIIAFGD
                     FITGENLVLSESENRKDEYIDFNSNRSNVISGIRNIFMAIFSPYIPMCGPLAATLTGS
                     VAQRYKIGKEAMQSVFSGMGTLVWVSAIFICFYPIAQIATPLIPLALSVTLLVQGYLC
                     TKLSMELCNTGVEKGLIGLMGGVIAAKGGTWGLAVGFILYFILIDSKKRKEKDINSIE
                     DCVYEILDKKKSI"
     gene            4342..4929
                     /locus_tag="KPD58_002219"
     CDS             4342..4929
                     /locus_tag="KPD58_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0265981.1"
                     /translation="MPPKVKITKEMILNTVLDITREVGFEAVNARSIASKLQCSTRPI
                     FTCYENMNELKNEFLAFAYEYYNQYVVNYRNSKSVSPYLLLPLSYIEFAQEETYLFKL
                     LFINDLDLMMKEANDFYKEIDNEKKARLFSESIGIELECAKVIFLDLFLYTHGIAVLT
                     ATKKLTLDRYSAEKMLKNILTAFIRQEKPDWNLSI"
     gene            4962..5753
                     /locus_tag="KPD58_002220"
     CDS             4962..5753
                     /locus_tag="KPD58_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG0265982.1"
                     /translation="METKQYLIDFYNTYDEDSRLALKHGMVEFLTTMHYIDKYIKSGD
                     CVLEIGAATGRYSHTLARQGYDVDAVELVEHNIEVFHKNTQSNENISITQGNAMDLSV
                     FPDNKYDITLLLGPLYHLYNKEDKQQALHEAIRVTKPGGVVFAAYVISDGCLIDEGFH
                     RGNINVSEYIEKGLIDPQTFAAKSEPKDLFELVRKENIDDLMSAFNVTRLHYVASDGL
                     ALYMREAVDSMDDDAFALYLKYHLATCEREDLVGVTSHAIDIFRK"
     gene            complement(5934..8351)
                     /locus_tag="KPD58_002221"
     CDS             complement(5934..8351)
                     /locus_tag="KPD58_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0265983.1"
                     /translation="MFRNNNRTFIRKIALNDLKINKLKTYLSGIIIMISTCLLLTVTL
                     VSYNASVDLVNASPYHAIYKSVDEKAKNILYKNKNFSGVGTYKLIGSNKKTDCIMSIV
                     YADDTTIKLMNFQPLKGKLPTNKNEIAISEKYLQEFGLDKDIGDSIKLNYYDDITNKE
                     VQCDFIIVGFLENYYQDNAKQYYTVVSNDYFKNIASTPLQNKSNTFDESRPDTVDVLV
                     KLSEENTEKDSASIKTQLKKIGLSLGIKKYNIYLNDNYIESNLIDGEQIITVLVVGIV
                     VLFSSVFVIYSIFYISVVNLVQMYAKLKSLGMTSFQLKKIIFLQGNILSIIFIPLGVI
                     ASCIIAYIIQPLAWQMIADLFIILILSLVMFLTVRISLFKPARIISKISAIEAMQYTE
                     TKFRKKVKNFSYINIKNLALKNIESNRKKNLIALISLSISGVLFISIANLANSIDFKK
                     QLSQQFVYNEDYIISIKSDNLYEHITEIQQNNPLTDSLKNEIKSIHGVKKLIESKLIK
                     SNIIEPYIKDRDEKQFITLIKGITPELSKSLEEYIVSGKIDYTHLDSDSLIINKYRTK
                     YYGLNLKVGDTVTFNIFSGNDIIKKKMKIIGIIDNSNTEGMFFASDKTLESLTPYNTN
                     LDFSILIDKKYSKEVEEKLLSIIQKNSNLMLYSYEDDYRMITRAFQYIIIASYIFVGL
                     ISCFGILNMTNTLINSVLIRKKEFALLQAVGMTRKQLRNMLYREGLNISIKAICTSSI
                     LGYFGSNLLCTFIKDVIRLDFINFKFSIFTILIFSFVLIGIQVLVTELLVRNIEKKSL
                     TERLRSQ"
     gene            complement(8364..9029)
                     /locus_tag="KPD58_002222"
     CDS             complement(8364..9029)
                     /locus_tag="KPD58_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0265984.1"
                     /translation="MSILQVINLKKYYGQSSNITKALDGVTLSIEKGEFISIVGTSGS
                     GKSTLLNMMGGLDNPTSGKVIIKGKELSTMTDEQLTIFRRRNIGFIFQNYNLVPLLNV
                     YENIILPIGLDGETIDKDFINEIINMLSLEEKLYDMPSNLSGGQQQRVAIARALVTKP
                     AIILADEPTGNLDSRTSSDVLSLLKVTSKKFNQTIVMITHNNEIAQLTDRIIRIEDGK
                     IVN"
     gene            complement(9111..10142)
                     /locus_tag="KPD58_002223"
     CDS             complement(9111..10142)
                     /locus_tag="KPD58_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0265985.1"
                     /translation="MNFKKFSFSSIYLIIAVGIIFTAILFSISIYLITADLKLIALLL
                     IYTIVLCGFIALIIFLLHKKIVIFCSKICHTLDSMMNSEDNIEIELEEETILSRINHR
                     LIRMYEVIQENRNSIAIEKADLQELVSDISHQIKTPIANLKMINTTLLNQTFKTEIQK
                     DFLMDMENQLDKLDFLMQSMVKTSRLETGIITLSKKKNSIYETIATALSGILFDAEKK
                     NIEVTVDCDPNLYIIHDKKWTSEAIFNILDNAVKYTNSNGKIRVATECWVMYTKIDII
                     DNGKGISESHQAEIFKRFYREEDVHDIEGIGIGLYLSRKIISLQGGYIKVTSDIGKGS
                     TFSIFLPNK"
     gene            complement(10132..10818)
                     /locus_tag="KPD58_002224"
     CDS             complement(10132..10818)
                     /locus_tag="KPD58_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0265986.1"
                     /translation="MEKILVVEDDSILNKTLSYNLIEDGYIITSKFTAKSALKSIFEC
                     EFDLIILDINLPDKSGFELCNEIKGNYNIPIIFLTANDMECDMIKGYELGALDYITKP
                     FNINIFKQKVKAFLNHLTIKTKQDYYRDGYLEINFSELSANINGNQIIFTPLEYRTLK
                     LLTENPKSILTRKVLLEKLWDIDANFVDEHTLTSVISRIRSKIEKDNLKYIKTVYGMG
                     YMWLGERNEF"
     gene            complement(10976..12040)
                     /locus_tag="KPD58_002225"
     CDS             complement(10976..12040)
                     /locus_tag="KPD58_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HBG0265987.1"
                     /translation="MKRVDERNTMFARANYKKDSTAYNDYYKKNPDKKEIDDSIRNRP
                     NLCSEGTMTYNELNSPMASSAFDFLSDIKSLCEGKVSDTKVDVDAKSMTKKIKGLAKQ
                     YGASVVGITKLKDYHIYTNRGRHEENYGEEINLTHKYAIVFGCEMDKEMINRAPMICE
                     VIETSKCYVDASIVGMILSYYIRNLGYDARNHMDANYLVMPVFIARDAGLGDIGRNAI
                     LTNKDYGSRLRLGVVTTDIPLLEDEYVDFGLEDFCKVCKKCSFNCPSHSLSNDIKIGD
                     DGKYNWVIEHETCYIKWRYLGTDCGMCISACPFSQNLETIKNTTSFKGNNELIQKALD
                     EYTSKFGKRIFIPGNPSWLK"
BASE COUNT         4484 a         1553 c         1748 g         4511 t
ORIGIN      
        1 gtttatatta aatttatttt attgatgaaa ctaatgggga ggttaaaact caatgattac
       61 ttttgatgta aatggagcta ttatcagatt tgatgataca aaaaataatt ataataagat
      121 aaggaaaatc tttaaattat atgctttaga aatatctaaa aactttgaaa gagattgttt
      181 aaatagtttt caaaatctaa agcaaatatc tgatagaggt ttaagtttag gagaaaaata
      241 tatagaagag tcttttaaga aaggaattga gactatagta agttttggaa ttataactat
      301 agatatcgat acctttaaaa gtgtttattg tgaaaaatat ttagatttta agagactttt
      361 taataatttg aataaaactt ttattatacc aaataaaaat aaaaaaaatg gccacgcgag
      421 cttcttagaa atagaatcga caataaagaa aatgagcaat tatttataca atgattgttt
      481 taaaattcat tatgctgttg tagatgccct tatagaaaat cgtataactg cggtacattc
      541 ttatattgat gaagaatcta taaaaaaatc taatgcctta tttaataatt ataaagatgg
      601 ttttattggg aaggctgacg aatgcaaagt agtaaaacaa ataataactt taaatccata
      661 tagggaagat gtatacgaat ttttggtaaa agaagatgga gattttagta aggaaattga
      721 gagattgact gaatttttag gctatgatat aagagaatat aaagcgtcat taatggatat
      781 ttatataaat gagttaatgg agagcgatga aaatgatgtt gaaaatgcaa aagaaaagat
      841 tgaaaaatat gctagatata ttggatgtag tgatgagaat ttatatgtag caagagttaa
      901 tgccatatat acttttgcaa atgcataatt ttattaaaca ttaaatatta gatgttcgat
      961 tatataaatt taaataaaaa aatatcctta ttaataacaa atagtttata ttaaaaacca
     1021 ttcttaaaat gagtggtttt ttattttgaa tagatatatt ttaaacctaa tagcattcct
     1081 atctttaatt agccaaaaat caactggatt tcatatagac tacataattt taaattacta
     1141 aaaaagacaa tatattgaca aagatggaat atgttgataa aatgaaatag tcaatatggt
     1201 gtcttaattt aaaaatttat ttatgttgaa ttagataaag ctgttttata taaaacataa
     1261 ataattatta taaggagaaa tagattatga aagtaaatgt atacaatgta gaatcatttg
     1321 tggatgaaag acttaagatt atggtttcgg acttaattcc aaatactaaa cttaaaataa
     1381 gtgtgaaaat ggaatttcca tggtgcaagg gagaagaatt ttcatcttat ggagtgtttt
     1441 gttcaaatga aaaaggagaa gtagatttag atttagttga acctatagaa ggtacttata
     1501 aggctgataa ttgtatgggg ttaatttatt cgctggaaaa atctaaaact gaaggtaaaa
     1561 attttgcaga aaatatatct attgataaac caataattat gaatatgata tttgaaactt
     1621 ctattgaaag aaaagaagtt gaattaaaga gaatatttaa atcagaagat attaccacaa
     1681 aatacatatc tgatgagttt ataggaaaat tattttataa acaaaattct aataataaaa
     1741 caattttaat gctaggtggt tctgatggaa atttagacgc tctagacctt ttagcagctc
     1801 cacttgcatc tagaggattc aatgtactaa cagttgcata ctttgcttta gaagggttac
     1861 cagataaatt agaagaggtt ccattagaat attttgaaaa agtatttgag tggattagtg
     1921 aaaatgaaat taccagtact aaagaaatat ttgtacatgg gacatcaaag ggtggagaat
     1981 tggccttttt acttgcttca agatatgaac aaattaaaaa ggttgtagta tcacaacctc
     2041 acatttattg tttccaagct cttaatggat tgatgagtgg aaatgatact tcatcatggt
     2101 catataaagg agagccattt ccatatatca aagtagacaa tgatatattc tttgaagaac
     2161 agaaaaaaaa tatatcaaaa gatattccat ttggatttaa tagtacttat aagaaaagtc
     2221 ttaaaagagc agagaataga aaagaggcta gaataaaaat agaaaattcc aaagcagata
     2281 tacttatgat tgctggaaaa gaagataaca tatggaatag ttatgaagct tgtttagagg
     2341 cattagaaat ctttgaagaa aataattatc catataacgt agaattatta acatacaata
     2401 atatgggtca tccgttacca attccatata ttatgcctct aaaggagact ttatgtatga
     2461 gtatgagtgg agggattttt tcttcaggtg gaactgtaga aggaaattca aaaggacagt
     2521 ttgaatcatg gaatagaact attgaatttt ataaaaaacc tttatcaagt gatttaaata
     2581 actaatatta agtgatttaa gtaactaaaa atgaagtggt ctttctatat atgcacaaaa
     2641 tctatcaatt ttatagattt aatttattgt ttatataatt tgcatgttat atactaattt
     2701 taatgacatt gataatttat aaacaattat aaagttgtaa acttacaaga gttattgaaa
     2761 ggtggagtga agttatggaa agactcgcta gaagggaaca acctcatata aaatttggaa
     2821 tgtttaagat aagaattcca tttatacatt acagatttga gaaaccagaa gctattcaag
     2881 gtataatatc aagtatgaca agtttaggca caattggcct tagtacacaa atattgggat
     2941 taccctatga agtggcatgg agtatggcta tcattaactc aatattatac tgtttgcatg
     3001 tttttatggg agacccagtt gtacctggct ggattacagc ttctataact cttacaactg
     3061 catatttgtt aaagttcagc atgggcattg aaagaataca agcattaaca gctttacaaa
     3121 tagatttagg aataatattt attttaatgg gtataactgg tgtagctgga aagttagtta
     3181 gtaagatacc aaattctatt aaaggtggaa tactaatggg tgtcggtata tcgactataa
     3241 tatcagagtt taatcctaaa gggagatttg attcttatcc tatatctata acagtaggaa
     3301 ttttggttgc ttgttttgta atgttttctg aaagatttga tactctaaaa gttaagaata
     3361 aatttttatt tcatttagga gaatatggtg tagtatcagc tattttagtg agtttaatag
     3421 taggtatatt tagtagagaa atagaaattc ctagatttag ttttgacaat ttagtttaca
     3481 tagtagattt taaaaatctg ataaatacag tatctccttt tggaataggt tttccaagtg
     3541 taatgttatt tatacaaggg ataccaatgg cttttatgat atacataatt gcatttggtg
     3601 attttataac aggtgaaaat ttagtacttt ctgaaagtga aaatagaaaa gatgaatata
     3661 tagatttcaa ttcaaatagg tctaatgtta taagtgggat aagaaatatt tttatggcaa
     3721 tcttttctcc atatatacca atgtgtggtc cattagctgc gacacttaca ggaagtgttg
     3781 ctcaaaggta caagattgga aaggaagcca tgcagtctgt ttttagtggt atgggtacat
     3841 tagtatgggt ttcagcaatt tttatctgct tttatccaat tgcgcagatt gctacacctc
     3901 ttataccatt agcattgtca gtaacacttt tagttcaagg atatttatgt acaaagctat
     3961 caatggagct atgtaataca ggtgttgaga agggattgat tggacttatg ggaggtgtaa
     4021 ttgctgctaa aggcggaaca tggggacttg ctgtaggttt tatactgtat ttcattttaa
     4081 ttgattctaa aaaaagaaaa gaaaaagata ttaattcaat agaagattgt gtatatgaaa
     4141 tattagacaa aaagaagagt atttaaagga aaatctgagt tgctgttatc tattaataga
     4201 taacagcaac tcatgtttta tttataagtt ttaggaattg caaaaaaatg caaagctttt
     4261 tcttgacata atttcttgat atgttactat agttatataa catatgtgtt ttataaaaat
     4321 ttaatggaga ggtgaacata tatgccacct aaagtaaaga taacaaaaga aatgattttg
     4381 aatacagtct tggatattac aagagaagtg ggatttgaag ctgtaaatgc gaggagtatt
     4441 gcaagtaaat tacagtgttc cactcgacca atttttactt gttacgaaaa catgaatgaa
     4501 ttaaaaaatg aatttcttgc ttttgcatat gaatattata atcagtatgt tgttaattat
     4561 cgtaattcta aaagtgtaag cccttattta ctgcttccgc tttcatacat tgaatttgct
     4621 caagaagaaa cgtatttatt taagttgtta tttataaatg atttagattt gatgatgaaa
     4681 gaggcgaatg atttttataa agaaattgac aatgaaaaaa aggcaagact tttttcggaa
     4741 agcattggaa tagagttgga atgtgcaaaa gtaatatttt tagatttatt tctttatact
     4801 catggcatag ctgtcttaac agcaacaaaa aaactaacat tagatagata tagtgctgaa
     4861 aaaatgttga aaaatatatt aacagctttt ataagacaag aaaaaccaga ttggaatttg
     4921 tctatttgat gtttattaac taatatagaa gaggtgaaat tatggaaaca aaacaatact
     4981 taattgactt ttataatact tatgatgaag atagtcgact tgcattaaaa catggaatgg
     5041 tggaatttct cactactatg cattacattg ataagtacat aaaatcaggt gattgtgtgc
     5101 ttgaaattgg cgctgcaact ggtcgttatt ctcacacact ggcacgtcag ggatatgatg
     5161 ttgacgcagt ggaattagtc gaacataata tagaggtttt ccataaaaat actcaatcaa
     5221 atgaaaatat aagcattact cagggtaatg ctatggattt atctgttttc ccagataata
     5281 aatatgatat tacgttactg ttaggaccac tatatcatct ttataacaaa gaagataaac
     5341 aacaagcttt acatgaggca attcgtgtaa caaaacctgg tggtgtggtt tttgctgcat
     5401 atgttatatc tgatggctgt ctcattgacg aggggttcca tagaggaaat attaatgtat
     5461 ctgaatatat tgaaaaaggt ttgattgatc cacagacatt tgctgctaag tctgaaccaa
     5521 aggatttgtt tgagcttgta cgtaaagaaa acattgatga tttgatgtct gcatttaatg
     5581 taactagact gcattatgtc gcatcagatg gtttggcttt gtatatgcgt gaagcagtag
     5641 actctatgga tgatgatgca tttgcattat acttaaaata tcaccttgct acttgtgaac
     5701 gtgaagattt agtaggggtt acaagtcatg caattgacat atttagaaaa taaggaatat
     5761 gcttgtttat gtctctggtg atgttgctcg gagatataag gaagtgtatc tgtataaaat
     5821 aaaaacaagt atagggatag aaaattatgt aaagtcaaaa ttttctatcc ttttagcttt
     5881 taaaaaatta tgttaaaact tgtataaaaa atggctattt tttatataca ttcttactga
     5941 gaacgtaagc gttctgtaag tgattttttt tctatatttc ttacaagtaa ttcagtcact
     6001 aaaacttgta taccaattaa tacgaatgaa aaaattaaaa tagtaaatat agaaaattta
     6061 aaattaataa agtctaatct aattacatct tttataaaag tgcaaagtag atttgaacca
     6121 aaataaccta gtatacttga agtacatata gctttgatac ttatgtttag accttctcta
     6181 taaagcatat ttcgcagttg ctttcttgtc attccaacgg cttgaagtaa ggcaaactct
     6241 ttttttctaa ttaaaacgct gtttattagg gtattggtca tatttagtat accaaagcaa
     6301 gaaattaaac caacaaaaat gtatgaagct ataattatat attgaaatgc tctagtaatc
     6361 attctataat catcttcata tgaataaagc attaaatttg aatttttctg aattatactt
     6421 aacaactttt cttcaacttc tttagaatac tttttatcta ttaaaataga aaaatctaaa
     6481 tttgtattat atggtgttag agattctaaa gttttgtctg aagcaaaaaa cataccttct
     6541 gtattggaat tgtcaatgat tcctataatt ttcattttct ttttaataat atcatttcca
     6601 ctaaagatat taaatgtaac tgtatctcca acttttaaat ttaatccata atattttgtt
     6661 ctatatttat ttataattaa actatctgaa tctaaatgtg tatagtctat ttttccactg
     6721 acaatatatt cttcaagtga ttttgaaagt tcaggcgtaa tacctttaat tagagtaata
     6781 aattgttttt cgtctctatc tttgatgtaa ggttctataa tattactttt aattaatttg
     6841 gattcaatta atttttttac tccatgaata gatttaattt cattcttaag agaatctgtt
     6901 agtggattat tttgttggat ttctgtaata tgctcatata agttatcact cttaatgcta
     6961 attatataat cttcgttata gacaaattgt tgagatagtt gttttttgaa atctattgaa
     7021 ttagcaagat tagcgattga aataaataaa actccactaa tacttagcga tattaatgca
     7081 atcaaatttt tctttctatt tgactcgata ttctttaatg ctagattttt tatatttata
     7141 taactaaaat tttttacttt ttttctaaat ttagtttctg tatattgcat ggcttcaata
     7201 gcagatattt tagaaataat tctagctggt ttaaataaag aaattctaac agttaaaaac
     7261 attaccagtg ataaaattaa aattataaat aaatcagcta tcatttgcca tgctaatggt
     7321 tgaattatgt aagcaattat acaagaagct ataaccccta atggaataaa aataattgac
     7381 aaaatatttc cttgtagaaa aataatcttt tttaactgga aagaagtcat acctaacgat
     7441 ttcaacttag catacatctg aactaaatta acaactgaaa tataaaaaat actatatata
     7501 acaaaaacac tagaaaaaag aacaactatt ccaactacta aaactgtaat aatctgctca
     7561 ccatcaatta aatttgattc aatatagtta tcatttaagt atatattata tttcttaata
     7621 cctaaagata aaccaatttt ctttaactgt gtcttaattg aagctgaatc tttctctgta
     7681 ttttcttctg atagtttaac taatacatct acagtatcag gtcttgactc gtcaaaagta
     7741 ttacttttat tttgtaaagg tgtagaagct atattcttaa aataatcatt tgaaacgact
     7801 gtatagtatt gcttggcatt atcttgataa taattttcta gaaatccaac aataataaaa
     7861 tcacattgaa cttctttatt cgtaatatca tcataataat ttaatttaat gctatctcca
     7921 atatctttat ctaaaccaaa ttcctgtaaa tatttttctg aaatagcaat ttcattttta
     7981 tttgtaggta gtttgccttt caatggttga aaattcataa gtttaattgt tgtatcatct
     8041 gcataaacaa tcgacataat acaatctgtc tttttattac ttccaattaa tttgtaagtt
     8101 cctacgccac taaagttttt atttttatat agtatatttt ttgctttttc atctacagat
     8161 ttatatatag catgatatgg agatgcatta actaaatcta cagaggcatt atatgaaact
     8221 aaggtcactg ttaataataa acatgtagaa atcattataa tgatacctga aagatatgtt
     8281 ttaagtttat taatttttaa atcatttaaa gctatttttc ttataaatgt gcgattatta
     8341 tttctaaaca tatactacac ctcctagtta acaatttttc catcttcaat acgaataatt
     8401 ctatcagtta gttgtgcaat ttcattattg tgtgtaatca ttacaattgt ttggttgaat
     8461 tttttacttg tcactttaag taaactcagt acatctgaac tagttctact atcaaggtta
     8521 cctgttggtt catcagctag tataatagct ggttttgtta caagtgctct agctatagca
     8581 acacgttgct gttgaccacc agaaagatta cttggcatat catataattt ttcttcaaga
     8641 gaaagcatat ttataatttc atttataaaa tccttatcta tggtctctcc atccaatcct
     8701 attggtaaga taatattttc atatacatta agtaaaggaa caagattata attttgaaat
     8761 ataaaaccta tatttctgcg tctaaagatt gtaagctgtt catcagtcat agtagataat
     8821 tccttaccct ttataataac ctttccagat gtaggattat ctaatcctcc catcatattt
     8881 aataatgtgg atttgccact accagatgta cctacaatag aaataaattc gcctttttca
     8941 atagatagag ttacaccatc aagagctttt gtaatgtttg aactttgtcc ataatatttc
     9001 tttaaattaa ttacttgtaa aatactcata ataatacctc cttaacatct tgtaaataca
     9061 gtataaaacc aaaatgttgc agaatcatta caaatgaaat atttaaaaat ttatttatta
     9121 ggtaagaaaa tagaaaaagt cgaccctttt ccaatatctg atgtaacttt aatatagcca
     9181 ccttgaagag atataatttt tcttgaaaga tagagaccta ttccaatacc ttctatgtca
     9241 tggacatctt cttcacgata aaatctttta aagatttctg cttgatgact ttctgatatt
     9301 ccttttccat tatcaataat atctattttt gtatacataa cccaacattc tgtagctaca
     9361 cgtattttac cattagaatt tgtgtatttt acagcattat ctaatatatt aaaaatagct
     9421 tcagaagtcc atttcttgtc atgaattata tataaatttg ggtcacaatc aactgtaact
     9481 tcaatattct ttttttcagc atcaaacaaa attccactaa gtgctgtagc aattgtttca
     9541 taaattgaat ttttcttttt agatagtgtg attatacctg tctctagtct ggaggttttt
     9601 accatagatt gcattaaaaa atctaattta tctaattggt tttccatatc cattaaaaaa
     9661 tccttttgaa tttctgtttt aaatgtttga tttagtagag tagtattaat catttttaaa
     9721 tttgcaatag gagttttaat ttgatgagat atatcagaaa ctagttcttg taaatcagct
     9781 ttctctatag caatactatt gcgattttct tgaataactt catacattct aattaatctg
     9841 tgattgatac gtgataaaat agtttcttct tctaattcaa tttcaatatt gtcttcacta
     9901 ttcatcatac tatctaaagt gtgacatatt ttagaacaaa atataactat ttttttatga
     9961 agtaaaaata ttattaatgc tataaatcca caaagcacta ttgtatatat taataataga
    10021 gcaattagtt ttaaatcagc agttattaga taaattgaga tactaaataa aatagctgta
    10081 aaaattatac caacagcaat tattaaataa atagaactaa aagaaaattt tttaaaattc
    10141 atttctttca cctaaccaca tatatcccat accatatacc gttttgatat acttaagatt
    10201 gtctttttca attttgcttc taatcctact tataacagat gttaaagtat gttcatctac
    10261 aaagttagca tctatatccc agagtttttc taaaagtact ttccttgtta aaatactttt
    10321 aggattttca gtaagcagtt ttaaagtacg atattctaat ggtgtaaata taatttgatt
    10381 tccatttata ttggcagata actcagaaaa gttaatttca agatatccat ccctatagta
    10441 atcttgtttt gtttttattg taagatgatt taaaaaagct ttaactttct gtttaaatat
    10501 attgatatta aatggttttg ttatataatc caaagctcca agttcatagc ctttaatcat
    10561 atcacattcc atatcattag cagttaaaaa aattattggt atattgtaat ttccctttat
    10621 ttcattacat aattcaaacc cacttttatc tggtaaatta atatccaaaa ttattaaatc
    10681 aaattcacat tcaaaaatag acttcagtgc agatttagct gtaaattttg aagtaattat
    10741 atatccatct tcaattaggt tatatgatag agttttattt aatatagaat catcttctac
    10801 tactaatatt ttttccatac agttactcct tttagtttaa tattttataa tgtcaattat
    10861 agactaaaaa cattgctgaa ttgttataaa cgagaaaaag tatttaaaaa tttatatatt
    10921 taataaagaa cctgtcctat gataaattct agtacagatt ctttattaaa taaaattatt
    10981 tcaaccaaga tggatttcca ggtataaata ttcttttacc aaattttgaa gtatactcat
    11041 ctaaagcttt ttgaataagc tcattattac ccttaaatga agtagtattc tttatagttt
    11101 cgagattttg gctaaatggg caagcagata tacacatacc acaatctgta cctaaatatc
    11161 tccatttaat atagcatgtc tcatgttcaa ttacccaatt atattttcca tcatcaccaa
    11221 ttttaatatc attagaaagc gaatgagaag ggcagttaaa agagcatttt ttacagacct
    11281 tacaaaaatc ttctagtcca aaatcaacat attcatcttc taaaagagga atatctgtag
    11341 taacaacacc taatctaagt ctagagccat aatctttatt cgtaagtata gcatttcttc
    11401 ctatatctcc taaaccagca tcacgagcta taaaaacagg cattaccaaa taattagcat
    11461 ccatatgatt tctagcatca tatcctaaat ttcttatata gtaacttaaa atcatgccga
    11521 cgatagatgc atccacatag cactttgatg tttctataac ttcacatatc attggagctc
    11581 tatttatcat ttctttatcc atctcacatc caaatacaat tgcatactta tgagttagat
    11641 taatttcttc accgtaattt tcttcatgtc tacccctatt tgtataaata tgatagtctt
    11701 ttaatttagt tataccaact acagaagctc catactgctt tgcaagaccc ttaatttttt
    11761 ttgtcattga ctttgcatct acatctactt tagtatcaga aacttttcct tcgcataaag
    11821 atttgatatc agataaaaaa tcgaatgcac tacttgccat tggagagttt aactcattgt
    11881 aagtcattgt tccttcacta cataaatttg gtctatttct tatactatca tcaatttctt
    11941 ttttgtcagg atttttctta taataatcat tataagcagt actatctttt ttgtaatttg
    12001 ctcttgcaaa catagtattt ctctcatcaa cccttttcaa ttctattccc cctagtaaaa
    12061 atatatttaa aaaatataac tttattgttg tataatttaa ttatataaga ataaagttat
    12121 attttcaatt taagcgtaat taaattggga aatatataac tatatgcttg attatgatgt
    12181 ttttattttg aaaacaaatt taaatacaat ctttttagtg aaataaataa aattaaaata
    12241 tttttagtat gataatgcaa gaaaataatt atttatctaa aaaatctaaa ggagag
//
LOCUS       DAELLV010000106        12237 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.106, whole genome shotgun sequence.
ACCESSION   DAELLV010000106 DAELLV010000000
VERSION     DAELLV010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12237
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.106"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            241..1029
                     /locus_tag="KPD58_002226"
     CDS             241..1029
                     /locus_tag="KPD58_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBG0265988.1"
                     /translation="MEKLQGKIAVVTAATKGIGLASAEILAKNGATVYLAARSEELAN
                     EVINKISAEGGCAKFVYFNAREEETFTSMIEEVVKKEGKIDILVNNFGSTNPSLDKDL
                     VTGDTDNFFDTVNTNLKSVYLPCKAAIPHMIKNGKGSIVNISSIGSVLPDLSRIAYCV
                     SKAAINSLTQNIATQYAKDNVRCNAVLPGLIATKAALDNMSPEFIKEFLKHVPLNRIG
                     EPDDIAKAVLFYASDDSSFITGDLLEVAGGFGLPTPQFADNILG"
     gene            1500..5216
                     /gene="rpoB"
                     /locus_tag="KPD58_002227"
     CDS             1500..5216
                     /gene="rpoB"
                     /locus_tag="KPD58_002227"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBG0265989.1"
                     /translation="MPHPVTIGKRTRMSFSKIKEIADVPNLIEIQVDSYEWFLKEGLK
                     EVFDDISPIEDYTGNLILEFVDYSLDDKPKYDIEECKERDATYCAPLKVKVRLINKET
                     GEIKEQEVFMGDFPLMTERGTFVINGAERVIVSQLVRSPGVYYAEERDKTGKRLISST
                     VIPNRGAWLEYETDSNDVISVRVDRTRKQPVTVLLRALGIGTDAEIIDLLGEDERLSA
                     TLEKDNTKTVEEGLVEIYKKLRPGEPPTVESASSLLNALFFDPKRYDLAKVGRYKFNK
                     KLALCYRIMNKISAEDIINPETGEVFVKAGEKISYDLAKAIQNAGINVVNLLMDDDKK
                     VRVIGNNFVDIKSHIDFDIDDLNIKEKVHYPTLKEILDGYSDEEEIKEAIKSRIKELI
                     PKHILLDDIIASISYEFNIFYNIGNIDDIDHLGNRRIRSVGELLQNQVRIGLSRMERV
                     IKERMTVQDMEAITPQALVNIRPVSAAIKEFFGSSQLSQFMDQTNPLSELTHKRRLSA
                     LGPGGLSRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLINSLGTYAKINEFGFIESP
                     YRKFDKETSTVTDEIHYLTADEEDLFVRAQANEPLTEDGKFVNHRVVCRTVNGAVEMV
                     PESRVDYMDISPKQVVSVATAMIPFLENDDANRALMGANMQRQAVPLVRREAPIIGTG
                     IEYRAAKDSGAVVVARNSGIAERVTADEIIIKREDGNRDRYNLLKFKRSNSGTCINQT
                     PIINKGDQIIKGDVIADGPATDLGEVALGRNCLIAFMTWEGYNYEDAILINERLVKED
                     RLSTIHIEEYECEARDTKLGPEEITRDIPNVGDSAIKNLDDRGIIRIGAEVDSGDILV
                     GKVTPKGETELTAEERLLRAIFGEKAREVRDTSLKVPHGESGIIVDVKVFTRENGDDL
                     SPGVNELVRCYIAKKRKIKVGDKMAGRHGNKGVISRVLPEEDMPFMENGTPLDIILNP
                     QGIPSRMNIGQVLEVHLGLAAKTLGWYVATSVFDGANEYDIMDALEEAGYPRDGKLTL
                     YDGRTGESFDNRITVGYMYYLKLHHLVDEKLHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAHILQEILTVKSDDVVGRVRTYEAIVKGENIPEPGIPESFKVLIK
                     ELQSLCLDVKVLTDEDQEIEVRESVDEDDTIGEFELDVVNHMGEVEESNIIEEIEDDF
                     AENAEDEDIENLEEFTEDDLFEEEIDFDSDDFDM"
     gene            5258..8743
                     /gene="rpoC"
                     /locus_tag="KPD58_002228"
     CDS             5258..8743
                     /gene="rpoC"
                     /locus_tag="KPD58_002228"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBG0265990.1"
                     /translation="MFELNNFESIKIALASPEKIRQWSRGEVKKPETINYRTLKPEKD
                     GLFCERIFGPQKDWECHCGKYRRVRYKGVVCDRCGVEVTKSKVRRERMGHIELAAPMS
                     HIWYFKGIPSRMGLLLDMSPRSLEKILYFASYVVVDPGETGLNEKQLLTEKEYRTALE
                     KYGYTFTVGMGAEAVKTLLQNIDLEQQSKDLRAELKDSTGQKKVRTIRRLEVVEAFKK
                     SGNKPEWMILDAIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELGA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKFYQCGLPKKMALELFKPFVMDKLVKEGYAHNIKSAKSIVEK
                     VKPEVWDVLEDVIKSHPVLLNRAPTLHRLGIQAFEPILVEGKAIKLHPLVCTAYNADF
                     DGDQMAVHVPLSVEAQAEARFLMLSVNNILAPKDGSPITTPSQDMVLGCYYLTIEAQD
                     GAKGTGMVFKDFNELLLAYYNKSVHLHALVKLKVTLEDGRSSLVESTVGRFIFNENIP
                     QDLGFVDRKENPFALEVDFLADKKSLGKIIDKCFRKHGNTETAELLDYIKALGFKYST
                     LGGITVAVDDMSVPEEKKVFIAEAEAKVDKYEKAYRRGLISDEERYEKVIETWTETTD
                     KVTDALMGGLDRLNNIYIMAHSGARGSKNQIRQLAGMRGLMANASGKTVEIPVKSNFR
                     EGLSVLEYFTSSHGARKGLADTAIRTAESGYLTRRLVDVSQDVIVREIDCGTEDTTEI
                     YAIKEGNEVIEEIYDRIVGRYTIDPILNPETGEVIVEADSMIQEDEAETIVALGIEKI
                     RIRTVLNCKTNHGVCSKCYGRNLATGKEVNIGEAVGIIAAQSIGEPGTQLTMRTFHTG
                     GVAGADITQGLPRVEELFEARKPKGLAVITEVSGRVEIDETGKRKEVNVIPEEGETQT
                     YVIPYGSRLKVKQGQMLEAGDPLTQGFINPHDIVRVNGVKGVQEYIVKEVQRVYRLQG
                     VDVNDKHIEVIVRQMLSKVKVEDPGDTDLLPGGYEDVLTFNECNKDAIDKGLRPAVAK
                     RVLLGITKASLATDSFLSAASFQETTRVLTEAAIKGKEDHLIGLKENVILGKLIPAGT
                     GMKKYRNIAVEKIED"
     gene            9032..9454
                     /gene="rpsL"
                     /locus_tag="KPD58_002229"
     CDS             9032..9454
                     /gene="rpsL"
                     /locus_tag="KPD58_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBG0265991.1"
                     /translation="MPTINQLVRKSRKALEKKSTAPALQKGYNSLNKKVTDASAPQKR
                     GVCTSVKTVTPKKPNSALRKVARVRLTNGIEVSAYIPGEGHNLQEHSVVLIRGGRVKD
                     LPGVRYHILRGTLDTAGVDKRRQSRSKYGAKRPKEAKK"
     gene            9578..10048
                     /gene="rpsG"
                     /locus_tag="KPD58_002230"
     CDS             9578..10048
                     /gene="rpsG"
                     /locus_tag="KPD58_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBG0265992.1"
                     /translation="MPRKGNIPKREVLPDPMYGSKVVTKLINNLMVDGKKGKSQRIVY
                     DAFAIVAEKTGEEALEVFNKAMDNIMPVLEVKARRVGGANYQVPIEVRPERRQTLGLR
                     WLVKYTRARGEKGMVEKLAKEIMDAANNTGASVKKKEDTHKMAEANKAFAHYRW"
     gene            10097..12163
                     /gene="fusA"
                     /locus_tag="KPD58_002231"
     CDS             10097..12163
                     /gene="fusA"
                     /locus_tag="KPD58_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBG0265993.1"
                     /translation="MARKFPLERTRNIGIMAHIDAGKTTTTERILFYTGQTHKIGETH
                     EGASQMDWMEQEKERGITITSAATTASWKDHRINIIDTPGHVDFTVEVERSLRVLDGS
                     VAVFCAKGGVEPQSENVWRQAETYGVPRIAFVNKMDILGADFYNVVSMMKSRLNSNAV
                     PMQLPIGKEDSFIGIIDLLKMDAVIYKDDLGVEMEETDIPEDMKELAAEWREKLVESV
                     AETDEELMMKYLEGEELTIDELKVAIRKATIACEMNPVFCGTAYRNKGVQLVIDAVLD
                     YLPAPTDIPAIKGILADGEEAERHSSDEEPFSALAFKIMTDPFVGKLAFFRVYSGTLE
                     SGSYVLNATKNKRERIGRILQMHANTREEITKVYAGDIAAAVGLKDTTTGDTLCDPAN
                     PIILESMEFPEPVISVAIEPSSKAAQEKMGIALQKLAEEDPTFTVKTDQETGQTIISG
                     MGELHLEIIVDRLLREFKVEAKVGAPQVAYRETITQPVDVEYKYSKQSGGRGQYGHVK
                     IRVAPQEPGEGYKFTNKTVGGSVPKEYVGPVDMGIQGAMQSGIVAGYPVVDVAVELYD
                     GSYHEVDSSEMAFKMAGSMAFKDAMKKGNAVLLEPYFKVEVVTPEDYMGDVMGDLNSR
                     RGLIQGMEARSGAQVINAFVPLSEMFGYSTDLRSSTQGRATYTMIFDHYEQVPASVAK
                     KIAEGK"
BASE COUNT         4744 a         1537 c         2504 g         3452 t
ORIGIN      
        1 ttttaattta acattattat caaattaaaa tataaacaat tgttgtaatg tctatatata
       61 aagtatataa ttaaactaaa atgtaaaatt ttacatttta gtttaaaaaa atacacgagt
      121 gttttgtaaa taaaatgtaa taaataatta attaacaaat ataaatttta aaataagtta
      181 gatatttaga aaaatcgaaa tttgtaggat ttaaaggaca tattcaagga ggagttaata
      241 atggaaaaat tacaaggaaa aattgcagta gttactgcag caacaaaagg tattggatta
      301 gcatcagcag agatattagc aaaaaatgga gcaactgtgt acttagcagc tcgttcagaa
      361 gaattagcta atgaagttat aaataaaata agtgcagaag gtggttgtgc taagtttgtt
      421 tactttaatg ctcgtgaaga agagaccttt acctcaatga tagaagaggt agttaaaaaa
      481 gaaggtaaga tagatatact tgtaaacaat tttggttcaa caaacccttc tcttgacaaa
      541 gaccttgtaa ctggggatac agataatttc tttgacacag taaatactaa tttaaaaagt
      601 gtgtatttac catgtaaagc agcaattcct catatgataa agaatggaaa aggaagtata
      661 gtaaatatat caagtatagg ttcagtatta cctgatttat ctagaatagc ttactgtgta
      721 tcaaaagcag caatcaactc attaactcaa aacatagcta cacaatatgc taaagacaat
      781 gttagatgta atgctgtact tccagggctt attgcaacta aagcagcatt agacaatatg
      841 tctccagagt tcataaaaga atttttaaag catgttcctt taaatcgtat aggggaacca
      901 gatgatatag caaaagctgt tctattctat gctagtgatg actcatcatt tataacaggg
      961 gatttacttg aagttgcagg aggatttggt ttacctactc cacaatttgc agataatata
     1021 ttaggataat taaattacta atatgcaatg aaagcttatg cattagttgt agtagaaatg
     1081 atatttttat aaagaccata gatataattt atattggaaa ggtttgaggt tcaaaattcc
     1141 aagttaaatt tatttatggt ctttattttg tatattttaa taaattgtat atatttgtat
     1201 aaatgtaatt atttacaatc attttaatat ggaattataa ttaaataggg gggatggtac
     1261 ttgtataaaa ttttttatag aaaattagac ctataaatga aaaatatgga ttttatattg
     1321 gaaaattgta cataatacat aatatatgct taaaataatt tacaaaaaat caaaaataat
     1381 gcatagttta catataagat aaaaaatata ttgaaaaata cttgacatta tgctaaaatt
     1441 attagatgcg ttagaatgaa ctattgtttt atttttaatt attgagaggt gaaaagtgaa
     1501 tgccacatcc tgtcacgata ggtaaaagaa ctagaatgag cttttctaaa attaaggaaa
     1561 tagctgatgt gccaaatctt atagaaattc aagtagattc ctatgagtgg tttttaaaag
     1621 aaggtttaaa agaagtgttt gatgatattt caccaataga agattatact ggtaatctta
     1681 tattggaatt tgtcgactat tctctagatg ataaaccaaa gtacgatata gaagagtgta
     1741 aagagagaga tgctacatat tgtgcacctc taaaggttaa ggtaagactt ataaataaag
     1801 aaacaggtga gataaaagaa caagaagtgt ttatgggtga cttcccttta atgacagaaa
     1861 gaggaacttt cgttataaat ggagcagaaa gagttatagt aagtcaatta gttagatctc
     1921 ctggtgttta ctatgcggaa gaaagagata aaacaggtaa gagattgatt tcatctactg
     1981 taattcctaa tagaggagca tggttagaat atgaaacaga ttctaatgat gtaatatctg
     2041 taagagttga tagaacaaga aaacaaccag ttacagtttt acttagagct ttaggaatag
     2101 gaacagatgc agagataatt gaccttttag gagaagatga gagattatct gctacattag
     2161 aaaaagataa tactaagaca gtagaagaag gtcttgtaga gatatacaaa aaattaagac
     2221 caggtgaacc tcctactgta gaaagtgcat catctttatt aaatgcttta ttctttgacc
     2281 caaaaagata tgacctagca aaagttggta gatataaatt caataagaaa cttgctttat
     2341 gttatagaat aatgaataaa atttcagcag aagacataat aaatccagaa actggagaag
     2401 tatttgtaaa agctggagaa aaaataagct atgatttggc taaagccata caaaatgcag
     2461 gtataaatgt agtaaatcta ctaatggatg atgataaaaa agttagagtt ataggaaata
     2521 attttgtgga tattaagtct cacattgatt ttgacataga tgatttaaac ataaaagaaa
     2581 aagtacatta tccaacttta aaagaaattt tagatggata tagtgatgaa gaagaaataa
     2641 aagaggctat aaaatcaaga attaaagaac ttataccaaa acatatatta ttagatgata
     2701 taatagcttc aataagttat gagttcaata tattctataa tataggaaat attgatgata
     2761 tagatcacct aggaaacaga agaataagat ctgtaggtga attgttacaa aatcaagtta
     2821 gaattggtct ttcaagaatg gaaagagtta taaaagaaag aatgacggtt caagatatgg
     2881 aagctataac gcctcaagcg ttagttaaca taagaccagt ttcagctgca ataaaagaat
     2941 tttttggtag ttctcagtta tctcagttca tggatcaaac aaatcctcta tctgagttaa
     3001 cgcataagag aagattatca gcccttgggc ctggaggtct ttcaagagaa agagctggat
     3061 tcgaagtgcg tgacgttcac cattcacact atggtagaat gtgtccaata gagactccag
     3121 agggaccaaa tatcggtctt ataaactctc taggaactta tgcaaaaata aatgaatttg
     3181 gatttataga atcaccatat agaaaatttg ataaagaaac atcaacagtt actgatgaaa
     3241 tacattattt aactgctgac gaagaagatt tattcgtaag agctcaggca aatgagccat
     3301 taacagaaga tggtaaattc gtaaaccata gagttgtttg tagaactgta aatggtgctg
     3361 ttgaaatggt tcctgaaagt agagtagatt acatggatat atctcctaag caggttgtat
     3421 ctgttgccac tgctatgata cctttcttag aaaatgatga tgccaaccgt gcccttatgg
     3481 gagcgaacat gcagcgtcag gcagtgcctc tagttagaag agaagcacca attataggaa
     3541 ctggtataga atacagagca gcaaaagact ctggagcagt tgttgttgct agaaattctg
     3601 gtatagcaga aagagtaaca gcagatgaaa taattataaa aagagaagat ggaaacagag
     3661 atagatataa tctacttaag tttaaacgtt caaactcagg tacttgtata aatcaaacac
     3721 ctataataaa taagggtgac cagataataa aaggtgatgt tatagcagat ggtccagcaa
     3781 ctgatttagg agaagttgca cttggaagaa actgtcttat agcattcatg acttgggaag
     3841 gttataacta cgaggatgct atattaataa atgaaagatt agttaaagaa gatagattat
     3901 caacaattca tatagaagaa tatgaatgtg aagctagaga tacaaaacta gggccagagg
     3961 aaataactag agatatacct aatgttggag atagtgcaat taagaattta gatgacagag
     4021 gtataataag aataggtgca gaagtagact caggtgatat attagttggt aaagtaactc
     4081 caaaaggaga aactgaactt actgctgagg aaagattact tcgtgcaata tttggagaaa
     4141 aggctagaga agttagagat acttcactta aagtgcctca tggtgaatct ggtataatag
     4201 ttgatgtaaa agtatttact agagaaaatg gagatgattt atctccagga gtaaatgaat
     4261 tagtaagatg ctatatagct aaaaagagaa aaataaaagt tggagataaa atggctggtc
     4321 gtcatggtaa taaaggggtt atctcaagag tattacctga agaagatatg ccattcatgg
     4381 aaaatggaac accactagac ataatactta acccacaagg tataccatca cgtatgaaca
     4441 ttggtcaggt tttagaagtt catttaggac ttgctgctaa gacattagga tggtatgtag
     4501 caacatcagt attcgatggt gctaatgagt atgacataat ggatgctctt gaagaagctg
     4561 ggtatcctag agatggtaaa ttaactttat atgatggtag aacaggtgag tcttttgaca
     4621 acagaataac tgttggatat atgtactact tgaaactaca tcacttagta gatgaaaaac
     4681 tacatgcaag aagtactgga ccatactcat tagttactca acagccacta ggtggtaaag
     4741 cacaatttgg tggacaaaga tttggagaga tggaggtttg ggctctggaa gcatatggag
     4801 ctgctcacat acttcaagaa atacttactg ttaagtctga tgacgttgta ggtcgtgtta
     4861 gaacatatga agctatagtt aaaggtgaaa atatacctga accaggaatc ccagaatcat
     4921 ttaaggttct tataaaagaa cttcaaagtt tatgcttaga tgtaaaagta ttgacagatg
     4981 aagaccaaga aatagaagta agagaatctg tggatgaaga tgatacaata ggtgaatttg
     5041 agctagatgt tgtaaatcat atgggagaag ttgaagaaag taatataata gaagaaattg
     5101 aagatgattt tgcagaaaat gcagaagatg aggatataga gaatttagaa gaattcactg
     5161 aagatgattt atttgaagaa gaaatagatt ttgatagtga tgactttgat atgtaaattt
     5221 aattaatgta aatctcaaat agaagggaga gaactccttg tttgaattaa acaatttcga
     5281 gtcgataaaa atagcattgg cttctccaga aaaaataaga caatggtcta ggggagaagt
     5341 taaaaagcca gaaactataa attaccgtac tttaaaacca gaaaaagatg gtcttttctg
     5401 tgaaagaata tttggaccac aaaaagactg ggagtgccac tgtggtaaat atagaagagt
     5461 tagatataaa ggtgtagttt gtgatagatg tggagtagaa gtaactaaat caaaagtaag
     5521 aagagagaga atgggacata tagagctagc ggctcctatg tctcacatct ggtacttcaa
     5581 aggtatacca agtagaatgg gacttttact tgatatgtca ccaagatcgt tagaaaaaat
     5641 attatacttt gcctcatatg tggtagttga tccaggagag actggattaa atgaaaaaca
     5701 attgcttaca gaaaaagaat acagaactgc tcttgaaaag tatggatata cttttactgt
     5761 aggaatgggt gctgaagctg taaagacatt actacaaaat atagatttag agcaacaaag
     5821 taaagaccta agagcggagt taaaagatag tacagggcaa aagaaagtta gaacaataag
     5881 aagattagaa gttgtagagg catttaaaaa gtctggaaat aaaccagaat ggatgatttt
     5941 agatgcaata ccagtaatac caccagattt aagaccaatg gtacaacttg atggtggaag
     6001 atttgcgact tcagacctaa atgatttata tagaagagtt ataaatagaa acaatagact
     6061 taaaagatta ttagagcttg gagctccaga tataattgta agaaatgaaa aaagaatgct
     6121 tcaagaagct gttgatgcat tgatagataa tggtagaaga ggtagacctg taacaggacc
     6181 tggaaataga ccacttaaat ctttatcaga tatgttaaaa ggtaagcaag gtcgtttccg
     6241 tcaaaactta cttggtaagc gtgttgacta ctcaggacgt tctgttatag ttgttggacc
     6301 agaacttaaa ttttatcaat gtggacttcc aaagaagatg gcattagaat tattcaagcc
     6361 atttgttatg gataaattag ttaaagaagg ttatgcacat aatataaaaa gtgcgaaatc
     6421 tatagtagaa aaagttaagc cagaagtttg ggatgtttta gaagatgtta taaaaagtca
     6481 tccagttctt cttaaccgtg caccgactct acatagatta ggtatacaag catttgaacc
     6541 aatcttagtt gaaggtaagg ctataaaact acatcctctt gtatgtacag cttataatgc
     6601 agactttgat ggtgaccaaa tggcagttca tgtaccttta tcagtagaag cacaagcaga
     6661 agcaagattc ttaatgcttt ctgtaaataa tatacttgct cctaaagatg gttcacctat
     6721 aactactcca tctcaggata tggttttagg ttgctattat ctaacaatag aagcccaaga
     6781 tggagctaaa ggaacaggta tggtatttaa agactttaat gaactattac ttgcttatta
     6841 caataaatca gttcatctac atgcattagt gaaacttaag gtaacacttg aagatggaag
     6901 aagttcattg gttgaaagta ctgttggtag atttatattt aatgaaaata tacctcaaga
     6961 cttaggtttt gtagatagaa aagaaaatcc atttgcactt gaagttgatt tcttagcaga
     7021 taaaaaatct cttggtaaaa taatagataa atgctttaga aaacacggaa atacagaaac
     7081 agctgaatta ctagattata taaaagctct aggatttaaa tattctacat taggtggcat
     7141 aacagttgct gttgatgata tgagtgtccc agaagaaaag aaagtattta tagctgaggc
     7201 agaagctaaa gttgataagt atgaaaaagc atacagaaga ggtctaatct ctgatgaaga
     7261 aagatatgaa aaagttatag agacatggac agaaacaact gataaagtta ctgatgctct
     7321 tatgggtgga ctagatagat taaataatat atatataatg gcacattcag gagccagagg
     7381 ttctaaaaac caaattagac agctggcagg tatgcgtggt cttatggcaa atgcatctgg
     7441 taaaacagtt gagataccag ttaaatctaa tttccgtgaa ggtttatcag tactagaata
     7501 ctttacatct tcacatggtg ccagaaaagg tcttgccgat acagctatac gtacagctga
     7561 atctggatat ttaacaagaa gacttgttga tgtaagtcaa gatgttattg taagggaaat
     7621 agattgtgga acagaagata ctacagagat ttatgctata aaagaaggaa atgaagttat
     7681 agaagagata tatgatagaa ttgtaggaag atatactata gaccctatat taaatcctga
     7741 aactggagaa gttatagttg aggctgattc aatgatacaa gaagatgaag cagaaactat
     7801 agtagcttta ggaattgaaa aaattaggat aagaacagtt cttaactgta aaactaatca
     7861 tggagtttgt tctaagtgtt atggtagaaa cttggcaaca ggaaaagaag ttaatatagg
     7921 tgaagcagtt ggtataatag cagctcaatc tatcggtgag ccgggtactc agcttacaat
     7981 gcgtacattc catactggag gagttgcagg agctgatata actcaaggtc taccaagggt
     8041 tgaagaatta tttgaagcta gaaaaccaaa aggattagct gtaataactg aagtatctgg
     8101 tagagttgaa attgatgaaa caggaaagag aaaagaagta aatgtaatac cagaagaagg
     8161 cgagactcaa acatatgtaa ttccatatgg ttctagatta aaagttaagc aaggtcaaat
     8221 gctagaggct ggagaccctc taacacaagg atttataaat cctcatgata tagtaagagt
     8281 aaatggtgtt aagggagttc aagaatatat agttaaagaa gttcaaagag tgtatagact
     8341 tcaaggggtt gatgttaatg ataagcatat agaagttata gtaagacaaa tgctatctaa
     8401 agtaaaagtt gaagacccag gagatacaga tttattgcca ggtggatatg aagatgtatt
     8461 aacattcaat gaatgtaata aagatgctat agataaaggt ctaagaccag cagttgctaa
     8521 aagagtttta cttggtataa ctaaagcatc tcttgcaact gattcattct tatcagcagc
     8581 ttctttccaa gaaacaacaa gagtattaac agaggcagca ataaaaggta aagaagacca
     8641 cttaatagga cttaaagaaa atgttatatt aggtaagtta ataccagcag gaacaggaat
     8701 gaagaaatat agaaatatag ctgttgaaaa aattgaagat taatagcata tagagtaaag
     8761 aaatcttgac aaccattatt tgtggtgtta aaatagtaaa gtgtgttgtt taaaagtaca
     8821 ctaagggctt gaacaagttc ttagtgactt ttagatggca ataaatgaga actaaaaagc
     8881 atcaagctag aaaagtgcta atatcacata tttatgtgtt atagacatta aattgtaagt
     8941 aacgaatgtt acaatcagtc agtatgaata ttcatattga tgtagttggc ggtcactgat
     9001 aagatcgaaa atttggaagg aggtgcagat gatgccaaca attaaccaat tagttcgtaa
     9061 aagtagaaaa gcgctagaga agaaatctac tgctccagca ttacaaaaag gttacaactc
     9121 tttaaataag aaagtaactg acgctagtgc cccacaaaaa agaggggttt gtacttcagt
     9181 aaaaacagtt actcctaaga agcctaactc agctttaaga aaagttgcca gagttagatt
     9241 aactaatggt atagaagttt ctgcttatat accaggagaa ggacacaact tacaagagca
     9301 tagtgttgtt cttataagag gaggaagagt aaaagacctt ccaggggtta gataccatat
     9361 actaagaggt actctagata cagcaggtgt tgataagaga agacaatcaa gatctaaata
     9421 cggtgcgaag agacctaaag aagctaaaaa gtaaaaacaa ttaatgattg attatagttc
     9481 ggtgctttaa atatatatat atttacggca tcacggcatt aaatgattta tgtcgagtac
     9541 ctatgattta agttaaataa ttaaggaggg aagcgaaatg ccaagaaaag gtaatattcc
     9601 aaaaagagaa gttttaccag atcctatgta tgggagtaag gtagttacta aattaataaa
     9661 taatctaatg gtagacggaa aaaaaggaaa gtctcaaaga atagtttatg atgctttcgc
     9721 aattgttgct gaaaagacag gagaagaagc tttagaagta tttaataaag caatggataa
     9781 tataatgcct gttttagaag taaaagcaag aagagttggt ggggcaaact accaagtgcc
     9841 aatcgaagtt agacctgaaa gaagacaaac attaggttta agatggctag taaaatatac
     9901 tagagctcgt ggagaaaaag gaatggttga aaagctagct aaagagataa tggacgcagc
     9961 taacaacact ggagcttcag ttaagaaaaa agaagatact cataaaatgg cagaagctaa
    10021 taaagcattt gctcattaca gatggtagga tatatagata tcttataaaa ttgaggatat
    10081 cataaggagg ataaccatgg ctagaaagtt tcctttggaa agaactagga atataggaat
    10141 catggctcat atagatgcag gaaaaactac tactacagaa agaatcctgt tttatactgg
    10201 gcaaactcat aaaataggag aaactcatga aggagcttct caaatggact ggatggagca
    10261 agaaaaggaa agaggtataa caataacttc tgccgctact actgcttcat ggaaagacca
    10321 tagaataaac ataatagata ctcctggaca cgtggacttc acagttgagg ttgaaagatc
    10381 tctaagagtt cttgacggtt cagttgctgt attctgtgca aaaggtgggg ttgagcctca
    10441 atcagaaaat gtatggagac aagctgaaac ttatggagta cctagaatag cttttgtaaa
    10501 taaaatggat atattaggtg cagatttcta caatgttgta agcatgatga aatctagatt
    10561 aaattctaat gcagttccta tgcaattacc aataggtaaa gaagattcat ttataggtat
    10621 aatagactta ttaaaaatgg atgctgttat atacaaagac gacctagggg ttgaaatgga
    10681 agaaacagat ataccagaag atatgaaaga attagctgct gagtggagag aaaaattagt
    10741 tgaatcagta gctgaaactg atgaagaatt aatgatgaaa taccttgaag gtgaagaact
    10801 tactatagat gaattaaaag ttgctataag aaaagctact atagcttgtg aaatgaaccc
    10861 agttttctgt ggtacagctt atagaaacaa aggtgttcag ttagtaatag atgcagtttt
    10921 agattactta ccagcgccaa ctgatatacc agctataaaa ggtatattag cagatggaga
    10981 agaagctgaa agacattctt ctgatgaaga gccattctct gcattagcat tcaaaataat
    11041 gactgaccca ttcgttggaa aactagcgtt cttcagagtt tattctggaa cattagaatc
    11101 aggttcttat gttttaaatg caactaagaa taagagagaa agaattggtc gtatattaca
    11161 aatgcatgct aatacaagag aagaaataac taaagtgtat gctggagata tagcagcagc
    11221 agtaggatta aaagatacta ctactggaga cacattatgt gaccctgcta atccaataat
    11281 acttgaatct atggaattcc ctgagccagt tatatctgtt gctatagaac caagttctaa
    11341 agcagctcaa gaaaaaatgg gtatagctct tcaaaaatta gctgaagaag acccaacttt
    11401 cacagttaag actgaccaag aaacaggaca aactataata tctggtatgg gtgaattaca
    11461 cttagagata atagtagata gattattaag agaatttaaa gtagaagcta aagttggtgc
    11521 accacaagtt gcatatagag agactataac tcaaccagtt gatgttgaat acaagtactc
    11581 taagcaatca ggtggtagag gacaatacgg tcacgttaag attagagttg ctcctcaaga
    11641 gccaggtgaa ggatacaaat tcacaaacaa aactgttggt ggatcagtac caaaagaata
    11701 tgttggtcca gttgacatgg gtatacaagg agctatgcaa tctggtatag ttgctggata
    11761 tccagttgta gacgttgctg ttgagttata tgatggttct taccatgagg ttgactcttc
    11821 tgagatggcg tttaagatgg ctggttcaat ggcattcaaa gatgctatga agaaaggtaa
    11881 tgcagttctt ttagagcctt acttcaaagt agaagttgtt actccagaag attacatggg
    11941 agatgttatg ggtgacttaa actcaagaag aggattaatc caaggtatgg aagctagaag
    12001 tggtgcacaa gttataaatg cattcgttcc actttcagaa atgtttggat actcaacaga
    12061 cctaagatct tcaactcaag gtcgtgcaac ttacacaatg atattcgatc actatgagca
    12121 agttccagct tcagttgcta agaaaatagc tgaaggaaaa taatttaaaa ataattaata
    12181 atataaatat aaaaaataat cagctagatg agagatatct ctcatctagc acaagat
//
LOCUS       DAELLV010000107        12107 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.107, whole genome shotgun sequence.
ACCESSION   DAELLV010000107 DAELLV010000000
VERSION     DAELLV010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12107
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.107"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(89..931)
                     /locus_tag="KPD58_002232"
     CDS             complement(89..931)
                     /locus_tag="KPD58_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG0265994.1"
                     /translation="MFKKKIGVLLASTMLVGALAVGCSSNSGGSGDSGDSSKVSVSGS
                     TSIGPLMETIGEKFQEKNQGVSVEVQQIGSSGGIKNAIEGTSEIGMSSRDLKDEEKSA
                     GLKETKIAIDGIALITNTANSVKNLTMDQVKGIYTGKITNWKEVGGNDAPIVIVSREE
                     GSGTRDGFQEIVGFDSEELTKDAQISDGSGNIKTTVEGNENAIGYISFGYVDEKVNSL
                     KINDVEATDANVIANKYPISRPFILVNKEDGLSEKGKALIDYILSDEGQALVSEKGFI
                     KVNQ"
     gene            complement(1183..2967)
                     /gene="pepF"
                     /locus_tag="KPD58_002233"
     CDS             complement(1183..2967)
                     /gene="pepF"
                     /locus_tag="KPD58_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBG0265995.1"
                     /translation="MGTDRSTIEEKYKWKIDKMYSSKEEIEKDISKVKNLIQEVKEYK
                     GKLSESKENLYKVLNISENASRIVQNLYVYTHMKQHEDTRINDNQGRATKTEMLSTDL
                     GVATSYIVPEILAIEENKLSEYLKDEKLSFYTKYIKDILRDKPHTLSEREEEILAATS
                     ELSTIPENVYDMLAYADMEFPEIEDEEGNKVKLSHSNYSLFIKSKNSRVRKDAFEGEF
                     STYEKYKNTFASTLYGGIKSEIFYAKTRKYNSAIEASLFSDDVSLDVYNNLISAIDKS
                     IPNLNKYVELKKKFLGLNEIHMYDLYVPLTDKFDMDIPYDKAQDIILEALKPLGEEYL
                     KVIKSAFDEGWIDVYDNEGKKGGAYSWGSYDSHPYILMSYKNDLNSLFTLIHELGHSV
                     HSHYSRTSQPFLYSDYKIFVAEVASTLNELLLINYLLENSKSKDETIYLLNYYLEQFR
                     TTVHRQTMFAEFEKIVHQRVESGEPLTADEFTNIYYKLNEKYYGKSAIVDKQIGIEWA
                     RIPHFYSNFYVYKYATGFSAASALSQQILSEGSTAVDRYINFLKSGGSEYPLEQLKSA
                     GVDMTKKESIEEALNVFAELVNKLEKEL"
     gene            complement(3033..3698)
                     /locus_tag="KPD58_002234"
     CDS             complement(3033..3698)
                     /locus_tag="KPD58_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBG0265996.1"
                     /translation="MIDIGSKEIAIRLLLALIIGGIIGLEREKIRQFAGFRTHILIAI
                     GSCIVSITSIQLFFDYNLYTNADPARMPAQVLSGIGFLGAGAILKNKGGVKGLTTAAG
                     MWATACIGIAVGYGYYELAILGGLFVMIALFVLRIINTFLFSQEKNVLILKLSNLDSI
                     PFLYDKFEKNQLIVRNMEIECRNEEYWKVNFFVSYAKRIHLHDVMKEINMIEGVINVD
                     YLD"
     gene            complement(3849..4010)
                     /locus_tag="KPD58_002235"
     CDS             complement(3849..4010)
                     /locus_tag="KPD58_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HBG0265997.1"
                     /translation="MANNELEALKNEIEAVREEINTYIEYPEIFQEELVESSKKIDIL
                     INKYMYLSK"
     gene            complement(4088..4597)
                     /locus_tag="KPD58_002236"
     CDS             complement(4088..4597)
                     /locus_tag="KPD58_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HBG0265998.1"
                     /translation="MKTRKMTFLGLMIGYSLALYVLETYIPNPLIVIFPGAKLGLSNI
                     ITLISLMLLGVKDTVIIVTIRVILSSIFAGPLSYLLFSIGGAYLSLLFMYLASKIKDL
                     SLIGISIIGAIGHNIGQLIVASLIIENILAIGYLPFMLMASLVTGMFVGLVSRYTIPK
                     LKHFYSKFY"
     gene            4803..5021
                     /locus_tag="KPD58_002237"
     CDS             4803..5021
                     /locus_tag="KPD58_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG0265999.1"
                     /translation="MTVYDLKIGEKGFINSIDGDIKLSKRLLALGCINSTEIEVKKVA
                     PLGDPIIIRFRGFDLAIRKSDAKNISLQ"
     gene            5039..6985
                     /gene="feoB"
                     /locus_tag="KPD58_002238"
     CDS             5039..6985
                     /gene="feoB"
                     /locus_tag="KPD58_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBG0266000.1"
                     /translation="MINVALLGNPNVGKTTVFNLLTGSNQYVGNWPGVTIEKKEGFLG
                     KEIKVVDLPGIYAMDTFSNEEKVSKSYLENEDVDVIVNVVDASNLSRNLYLTTQLMQF
                     NKPIVILLNMLDIAESKGVNIDAKKLSEELGVIVVPIIAKKKNGVDRIEEEIKGATQK
                     AFIYQKNFGSETETYKKLESILSRCTKTTYTQKKSISDKIDNIVLNPILAYPIFIGVL
                     FLLFKFTFDWVGGPLQEGFASLIEAYISAPVSDMLSNSSPWFKSLIVDGIIGGVGGTL
                     PFFPLIFTLFFGISLFEDSGYMSRTAFLMDKVMRKVGLSGKAFIPIVMGMGCSSPAIM
                     ATRTLESEKDRRVTALIAPLMTCGAKLPIYALFVAIFFPHNAALVTTSLYLVGIVMAI
                     VVALFLNKTAFKTEAEPFILELPEYRIPTIDALIKNTWNKSKGFLIRVVTVMFAMSVV
                     IWGLSSFNVFGYTEDINVSFLATLGHIISPIFKPLGFDDWRTSVAILSGLGAKEIVVN
                     SLNILYGNLTVVLPTIFNGVTAYTFLIFTALYTPCIAALATMKKEYGNKMMFTSFAYQ
                     FILAWIMAFIVKSIGGVLFMGNSIIEALIGGIIIVLALFILFNKFKSTKNGNVCSGCS
                     ICSGCPSANSCSSKEDSNKKNNAH"
     gene            complement(7164..8219)
                     /locus_tag="KPD58_002239"
     CDS             complement(7164..8219)
                     /locus_tag="KPD58_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG0266001.1"
                     /translation="MTIQDYMLETPVRMREIISNADSLFNEVKRTNLKKIIITGSGTS
                     YHSGVQVQPYLQNLLDIDVVKMYPFMITEDTFKFDNENTLVVGVSQGGSSYSTYNAMK
                     LAEDKGCKIASMAGCKNALIDEISDYILTVNCGEEKSGAKTKGYYCTKLNLMLLGLQI
                     AREKGIISSEKYNEEINKILDAINRFEAVYKLSKQWIERNKEKLVNSKEIRIIGHSDI
                     YGDTLEAALKLLETMRIPVTGYEFEEFIHGIYNAINSDSTIFILDTGKEPRVTKMIDV
                     LSGWTENVFAIGRDVTENDKNLKIDITDNPYYQTFNFIVPIQLICGEIPTLRGVDPSV
                     PKDTRFHMKLGSKKLNK"
     gene            complement(8267..9067)
                     /locus_tag="KPD58_002240"
     CDS             complement(8267..9067)
                     /locus_tag="KPD58_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG0266002.1"
                     /translation="MNKLTKKELRSMFWRSFVLQGAFNYERMQNLGYCYSMLPAIKKL
                     YNKKENQAKAIERHLEIFNTTTVVVPAILGITAAMEEENANNPEFDESSISAVKTALM
                     GPLAGIGDSLFWGTFRIIAAGVGVSLAKEGNIFGPLLFLLLYNIPAFALRIFGLKYGY
                     QVGVNSLERIQREGLMEKIMSMATTVGLFVVGGMVATMLSITTPLKFNLNGAEVILQD
                     ILDKIIPNMLPLTFAFVIYYMLKRKVSVTKLTIGTIVTGIALHAIGLL"
     gene            complement(9060..9851)
                     /locus_tag="KPD58_002241"
     CDS             complement(9060..9851)
                     /locus_tag="KPD58_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0266003.1"
                     /translation="MLVQATMLAIIAGVGILDGRIFGQSMLDRPIVTGMLVGLVLGDI
                     KSGIMIGAQLELIWMGIAGIGAATPPDVVTGGVLGTAFAILSGNGAEVALAVAVPVAV
                     LAQSLGVLVRIINSYFSQKAVFYAKQADFKKVTIMMWLPVILFFLSTSVPTFLAIMIG
                     ADKVTALINAIPKVILDGLGVAGTLLPAVGFALLMDMLLSKKMSVFFFIGFLLASYGG
                     LDITAIALFGVCVAFVLNFYINNKENNQNPQLAINTLTEGEIDFE"
     gene            complement(9891..10313)
                     /locus_tag="KPD58_002242"
     CDS             complement(9891..10313)
                     /locus_tag="KPD58_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HBG0266004.1"
                     /translation="MKKFLIATHGELSRELVDTSKLIIGSLTNIEYFCMTTDKSADDA
                     EKEIRSILSNNKDSEEFVVLTDVFGGSVANICTNLLLQGFKFELLTGVNLPMLLSILL
                     LVEEDTKLIVKSGLEEAKNGIVYVNELLENQNKIKNVM"
     gene            complement(10329..10808)
                     /locus_tag="KPD58_002243"
     CDS             complement(10329..10808)
                     /locus_tag="KPD58_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0266005.1"
                     /translation="MVSLMRIDDRLIHGQVAYGWATSLGINVILVANDEAKNDQMKAM
                     SLNLAKPSNVTLYIRGVEESGEIVEKFASSKKSNVLVLVKNTKDALVVAKNSGGVLKE
                     INVGGLRYEEGKKKLTDLVAVDDDDINNFKQIGDLGIDIEFRMLPRDKKKQLSDLIK"
     gene            complement(10940..>12107)
                     /locus_tag="KPD58_002244"
     CDS             complement(10940..>12107)
                     /locus_tag="KPD58_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG0266006.1"
                     /translation="TNRLDMYLNNVDTIIMIIFIKSTIIRMKFNQVNATIIAHGYATA
                     SSMANVCNRMLGKNIFTAIDMPIDATIADVSKQMSRYLEGDRIKNGLIVLFDMGSLNL
                     IFEDLKNKINSPMLFIDQISTISALEVGNLLMQGKNIEEIAEIMDEETKPNIQLYYPQ
                     KNKEYAIISSCFTGIGTAIKIQHLLSESLIGIVDVKIIPYDYDSLVSMGDKEPVFEMY
                     DVMAIVGTANPNVNGVDFILLEDIISGKGEDDVFRIFSRVVENEKIKNINDSIVKNFS
                     LIRVIDSLTILDSKKIIERIEEGINAIENIQKKKLSNDKKISLYVHLSCMIERLVRQT
                     EIEEYTDMDLLIKNHHSEVKLIKNAFSVLEKSYSVQIPISEIGYIYNIIYGLPQ"
BASE COUNT         3909 a         2118 c         1435 g         4645 t
ORIGIN      
        1 taatagacaa taccctgcaa gttaaagcat catttttatg agcttatctc acagggtatt
       61 atatgtttac tttatattat aactcttgtt attgattaac tttaataaat cctttttctg
      121 aaacaagtgc ttgtccttca tcacttaata tataatctat taaagcttta cctttttctg
      181 acaaaccatc ttctttatta actaatataa atggtcttga tattggatat ttattagcta
      241 ttacatttgc atcagttgct tcaacatcat ttatttttaa actatttact ttctcatcaa
      301 catatccaaa tgatatatat cctatagcat tttcatttcc ttcaactgta gtttttatat
      361 ttcctgaacc atcacttatt tgtgcatctt tagttagttc ttctgaatca aaccctacaa
      421 tttcttggaa tccatctcta gttcctgaac cttcttctct tgatactatt actattggtg
      481 catcatttcc tcctacttct ttccagttag taatctttcc tgtatatata cctttaactt
      541 ggtccattgt cagatttttt actgagtttg cagtattagt tatcaatgct ataccatcta
      601 ttgctatttt agtttccttt aatccagcac ttttttcttc atcttttaaa tctcttgaac
      661 tcattcctat ttcagatgtt ccttctatag cattttttat acctccagaa gaaccaatct
      721 gttgtacttc tacactaaca ccttgatttt tttcttgaaa cttttctccg atagtttcca
      781 taagaggacc tattgaagtt gaacctgaaa cagaaacttt gcttgaatct cctgaatcac
      841 ctgaacctcc tgagttagaa ctacatccta ctgctaaagc tccaactaac attgtactag
      901 ctaaaagaac gcctattttc tttttaaaca ttttacttac ccccgcttat attttaattt
      961 tttgttgtca tttttctata ttttaataat acagattctt tgttaaatga atattagagc
     1021 agggttaagt aaatgtaaat attgttaaat acatgtaaaa acttaatttt atatgattta
     1081 ttcttaatta gattaacttt ttaaagttac tttaggcaga tataaacata atacaactca
     1141 tattattaga agtcttcatt gaagctatca acctatcaca tcctataatt ctttttctag
     1201 tttattaact aattcagcaa atacatttaa ggcttcctct atagattctt ttttcgtcat
     1261 atctacacca gcagatttta attgttctag tgggtattct gaccctccac tttttaagaa
     1321 gtttatatat ctatctactg cagtacttcc ttctgaaagt atctgttgac ttaaagcact
     1381 tgcagcagaa aaccctgtgg catacttata tacatagaag tttgaataaa aatgaggtat
     1441 tctcgcccat tctattccta tttgtttatc tactattgca gattttccgt aatacttttc
     1501 atttaatttg tagtatatat tagtaaattc atcagcagta agtggttctc cactctctac
     1561 cctttgatgt acaatttttt caaattcagc aaacatagtt tgtctatgaa ctgtagttct
     1621 aaattgttct aaataataat ttaataagta tatagtttca tccttagact tagaattttc
     1681 taataaataa tttataagaa gtaattcatt taatgtagat gcaacttctg caacgaatat
     1741 cttatagtcc gaatataaaa atggttgact tgttcttgaa taatggctgt gaacagagtg
     1801 ccctaattca tgaattaaag tgaatagaga attaaggtca tttttatagc tcattaatat
     1861 ataaggatgt gaatcataac ttccccaaga ataagctcct ccttttttac cttcattatc
     1921 atatacatca atccaccctt catcaaaagc actttttatg accttcaagt attcttctcc
     1981 taaaggttta agtgcctcta atatgatatc ctgtgcttta tcatatggta tatccatgtc
     2041 aaatttatct gtcaaaggaa catacaaatc atacatgtgt atttcattca atcctaaaaa
     2101 tttcttttta agctcaacat atttatttag attaggaata cttttatcta tagctgatat
     2161 aagattattg taaacatcta aacttacatc atctgaaaat aaagacgctt ctattgctga
     2221 attatacttt ctagtttttg cgtaaaatat ctctgatttt attccaccat aaagcgttga
     2281 ggcaaatgta tttttatatt tctcatatgt tgaaaattca ccttcaaaag cgtcttttct
     2341 aactctacta ttcttacttt ttataaataa agagtagttt gaatgagaaa gcttaacctt
     2401 attcccctcc tcatcttcta tctcaggaaa ttccatatct gcatatgcta acatatcgta
     2461 tacattttct ggaattgtcg ataattctga tgtagctgca agtatttctt cctctcgttc
     2521 actcaaagta tgtggtttat ctctcaatat atcctttata tattttgtat aaaaggatag
     2581 cttctcatct tttaagtatt cacttaactt attctcttct atcgccagta tttctggaac
     2641 tatataagaa gtagccacac ctaaatcagt agataacatt tctgtcttag tggctcttcc
     2701 ttgattatca ttaattctag tatcttcatg ttgtttcata tgagtatata catataaatt
     2761 ttgaacaatc ctagatgcat tttcagagat atttaatact ttatataagt tctccttact
     2821 ctcagaaagc ttccctttat attcttttac ttcttggata agatttttta ctttacttat
     2881 gtctttttca atttcctctt tactggagta catcttatct atcttccatt tgtacttttc
     2941 ttctattgta cttctatcag ttcccatatc atacacctct attttaattt gttttatatt
     3001 ttattcactt tacaaaatat attgtatata ttttagtcca aataatctac atttattact
     3061 ccttctatca tatttatttc tttcataacg tcgtgtaagt gtattctctt agcataagat
     3121 acaaaaaaat taaccttcca gtattcttca ttacgacatt ctatttccat atttcttacg
     3181 ataagttgat ttttttcaaa tttatcatat aaaaaaggta tagaatccaa attgcttaat
     3241 tttaatatca atacattctt ttcttggcta aataaaaacg tattgattat cctaagcaca
     3301 aataatgcta tcattacaaa taatccacct agaattgcca attcataata accatatcca
     3361 actgctatac caatacatgc agttgcccac atcccagcag ctgtagtaag cccttttact
     3421 ccacctttat tttttaaaat tgctcctgca cctaaaaaac caattccaga taatacttga
     3481 gcaggcattc ttgctgggtc agcatttgta tacaaattat aatcaaagaa taattgaatc
     3541 gaagttattg aaacaataca agaacctatt gcaattagta tatgtgtcct aaatcctgca
     3601 aactgtctta ttttttctct ctctaagcct attattcctc ctattattag tgctaatagt
     3661 aaccttattg ctatctcctt acttcctata tcaatcataa tataaaaccc tcctgactta
     3721 attacaactt atgtcctcca gataatttac acatcagaaa tactatttta tatcttaata
     3781 ttattattaa tatatgtaaa atatagccat ctgttaattt ttgatacaaa aaaacccaca
     3841 actgtgggct atttgcttag gtacatgtat ttatttatta atatatctat tttcttactt
     3901 gattctacta actcttcttg aaatatttct ggatattcta tgtatgtatt tatctcttcc
     3961 ctaactgctt caatctcatt ttttaacgct tctaattcat tgttcgccat aataatcacc
     4021 actattacaa atttgtatat ttttatatac attttatcaa acaatattat attttgtcaa
     4081 tattatgtca ataaaactta ctataaaaat gctttaattt aggtattgta tacctggata
     4141 ctaagcctac aaacatacca gttactaatg atgccattaa cataaatggt aagtacccta
     4201 tagcaagtat attttcaatg attaaacttg caactattaa ttgacctata ttatgcccta
     4261 ttgcacctat aatgcttatc cctattagtg ataaatcttt tattttactc gctaaataca
     4321 taaataaaag acttagataa gctcctccta tactaaatag tagatatgat aaagggcctg
     4381 caaatattga tgacaaaatt accctgatag taacaattat aacagtatct ttgacaccta
     4441 acaacattaa tgaaattaaa gttataatat tgctcaaacc caattttgcc cctggaaata
     4501 taactataag aggatttggt atatatgtct ctaatacata taaagctaaa ctatacccta
     4561 tcataagacc taaaaatgtc atttttctag ttttcattaa gttaactcct ccaaatttta
     4621 tctatcatct tacattataa tacaaaatat ataaaaacac ctaatttaga ctaaaatatt
     4681 tttaatctat ttagaataaa tattagttat ttcaatgtgc aattacctct ataaatcttg
     4741 actttttgta tcatacttgt tacaatttta atgataatga atttcattag gaggtgttaa
     4801 taatgactgt ttacgattta aaaataggag aaaaaggatt tattaatagt atagatggag
     4861 atattaaatt atctaaaaga ttgcttgcac ttggatgtat aaatagtact gaaatagaag
     4921 ttaaaaaggt tgccccactt ggagacccaa ttataattag atttagaggc tttgaccttg
     4981 cgattagaaa atctgatgct aaaaatattt ctttacaata atagaggagg agctacccgt
     5041 gattaatgta gcattattag gaaacccaaa tgtcggtaaa actactgttt ttaatctact
     5101 tacaggttca aatcaatatg taggtaactg gcctggtgtt actatagaaa aaaaagaagg
     5161 atttcttgga aaagagataa aggttgtcga tttacctggt atatatgcaa tggatacttt
     5221 ctcaaatgaa gaaaaggttt ctaaatcata tttagaaaat gaagatgttg atgtaatagt
     5281 aaatgtagtt gatgcttcta atttatcaag aaacctttac ttaacaactc aacttatgca
     5341 atttaacaag cctatagtta tattgctgaa tatgttagac attgcagaat ctaaaggagt
     5401 taatatagat gccaaaaaat taagtgaaga acttggagta attgttgttc caattatagc
     5461 aaaaaagaaa aatggtgttg ataggataga agaagagatt aagggagcta cgcaaaaagc
     5521 ttttatatat cagaaaaact ttggaagtga aacagaaaca tataaaaaat tagaatccat
     5581 tttatcaaga tgtacaaaaa ctacatatac acaaaagaaa tctatcagtg ataagataga
     5641 caatatagtc ttgaatccta tattagcata tccaatattt attggtgtat tgttcctctt
     5701 atttaaattt acctttgact gggtcggagg tccactacaa gagggatttg ctagtcttat
     5761 agaggcttat atttctgcac ctgttagtga tatgttatct aactctagcc catggtttaa
     5821 atcacttata gttgatggaa taattggagg tgttggtggt actttaccat tcttcccttt
     5881 aatatttaca ttattctttg gtatatcgtt gtttgaagat agtggatata tgtcaagaac
     5941 agcattttta atggataaag ttatgagaaa ggttggtcta tctggtaaag cttttatacc
     6001 tatagttatg ggtatgggat gttcttctcc agctataatg gcaactagaa cattggaaag
     6061 cgaaaaagat agaagagtta cagctcttat tgcaccactt atgacttgtg gagcaaagct
     6121 acctatatat gcactatttg tagctatatt ctttccacat aatgctgctt tagtaactac
     6181 atcactgtat ttggtgggta tagttatggc aattgtagtt gcattattct taaataaaac
     6241 agcctttaaa acagaggctg aaccatttat attagaatta ccagaatata gaatccctac
     6301 tatagatgca cttattaaaa atacttggaa taaatcaaaa ggtttcttga taagagttgt
     6361 tactgtcatg tttgcaatgt cagtcgtaat ttggggactt tcttcattca atgtttttgg
     6421 atatacagaa gatataaatg taagcttctt ggctactcta ggtcatatta tatctcctat
     6481 attcaaacct cttggttttg atgattggag aacttcagtt gctattttaa gtggtcttgg
     6541 agcaaaagaa atcgtagtaa actctttaaa tatactatat ggtaatctaa ctgttgtctt
     6601 accaacaata tttaatggag ttactgcata tacattcttg atatttactg ctttatatac
     6661 tccatgtata gctgcacttg caactatgaa aaaagaatat ggcaataaaa tgatgtttac
     6721 atcatttgca tatcaattta tactagcatg gataatggct tttatagtta aaagtatagg
     6781 tggagtacta tttatgggta attcaataat agaagctttg ataggtggaa taattatagt
     6841 ccttgcactc tttattctgt ttaataaatt caagtctaca aaaaacggaa atgtctgtag
     6901 tggatgttct atttgttctg gatgtccaag tgctaattct tgctcaagta aggaagacag
     6961 caataaaaaa aataatgcac actaatatat aaatatttta gtttaaaata agataaaagt
     7021 gggtgtctca aaattaactt tttagttcat gaggcactct tttttataga aaatcaaata
     7081 tattttcatc atttcaacag taaaaaagag cttatctagt aaaaaagagg cttatctcta
     7141 ttttgagaca acctcttttt ttgttactta ttcagtttct tgctacctaa cttcatgtgg
     7201 aacctagtat cttttggaac acttgggtct acacctctta atgtaggtat ttccccacat
     7261 attagttgta taggtactat aaaattaaat gtttgatagt aaggattatc tgtgatatca
     7321 atctttaaat ttttatcatt ttctgtaaca tctctaccta tagcaaatac attttccgtc
     7381 caaccagaaa gtacatcaat catcttagtt actctaggtt cttttcctgt atctagtata
     7441 aatatagtag aatcactatt tatagcatta tatataccat gtataaactc ttcaaactca
     7501 tacccagtta caggtattct cattgtttcc aataatttta atgctgcttc taatgtatct
     7561 ccatatatat cagagtgacc tattattctt atttctttag aattaactaa cttttctttg
     7621 tttctttcaa tccattgttt agagagctta tacactgctt caaatctatt aattgcgtct
     7681 aatatcttat ttatttcctc attgtacttt tcactagata ttataccttt ttctctagct
     7741 atttgaagac ctaataacat taagtttaac tttgtacaat aatatccttt tgttttagct
     7801 ccagattttt cttcaccaca atttactgtt aatatataat cagaaatctc atctattaaa
     7861 gcattcttac aacctgccat agatgctatt ttacagccct tatcttcagc taacttcata
     7921 gcgttgtatg tagaataact actcccacct tgagatacac ctactactag ggtattttca
     7981 ttatcaaact tgaatgtatc ttcagttatc ataaatggat acattttcac aacatctata
     8041 tctaataaat tttgtaaata tggttgcact tgcactcctg agtgatagct agtacctgaa
     8101 cctgttatta taatcttttt tagatttgtt ctcttaactt cattaaataa gctatctgca
     8161 ttagatataa tttctctcat tctaactggt gtttctaaca tataatcttg tattgtcatt
     8221 ttatacacct tccttatcta tatattcaat aaattataat taatttctat aacaaaccta
     8281 tagcatgtaa agcgattcct gttactatag tgcctatagt taattttgtt acacttacct
     8341 ttctctttaa catgtagtat attacaaatg caaaggttaa aggtaacata ttaggtatta
     8401 ttttatctag tatatcttgt aaaattactt ctgcaccatt taagttgaat ttcagaggag
     8461 tagttatact caacatagtt gcaaccattc ctcctacaac gaataaacca acagttgtag
     8521 ccatagacat tattttctcc ataagtccct ctctttgtat tctttcaaga gaatttaccc
     8581 ctacttgata accatactta agtccaaata ttctaagggc aaatgctggt atattgtata
     8641 atagtaagaa taatagtggt ccaaagatat taccttcttt tgcaagtgat actcctactc
     8701 ctgctgctat aatcctaaat gttccccaga ataatgaatc accaattcct gcaagaggtc
     8761 ccataagagc tgttttaaca gcactaattg aactttcatc aaattctgga ttattagcat
     8821 tctcttcttc catagctgct gttataccta atatagcagg aactactact gtcgttgtat
     8881 taaaaatctc caaatgcctt tctattgctt ttgcttgatt ttctttctta ttgtatagct
     8941 tctttattgc aggtaacatt gaataacagt aacctaagtt ttgcattctt tcgtaattaa
     9001 atgcaccttg aagaacaaat gaacgccaaa acatacttct taattctttt tttgttaatt
     9061 tattcaaagt caatctctcc ttccgttaaa gtattgattg ctaattgagg attttgatta
     9121 ttttctttgt tattgatata aaagtttagt acaaatgcta cacatactcc aaataaagct
     9181 atagcagtta tatctaatcc accataagat gctaacaaaa accctataaa gaaaaataca
     9241 gacatctttt ttgaaagtaa catatccata agcaatgcaa aacctactgc tggcaacaat
     9301 gttcctgcaa cacccagtcc gtctaatata actttaggta ttgcatttat caatgctgta
     9361 actttatcag caccaatcat aattgctaaa aaggttggaa ctgatgtact taaaaagaat
     9421 aatattactg gcaaccacat cattatggta actttcttaa aatcagcttg tttagcatag
     9481 aaaactgcct tttgagaaaa gtacgaattt attattctaa ctaatactcc aagagattgt
     9541 gctaagactg ctactggtac tgctactgct aaagcaactt ctgcaccatt acctgataat
     9601 atagcaaaag ctgtacctaa tacgcctcct gttactacat ctggaggagt agctgcacct
     9661 attcctgcaa ttcccatcca aattaattca agctgtgcac caatcataat tcctgatttt
     9721 atgtctccta aaactaatcc tactaacata cctgtaacta ttggcctatc aagcatagat
     9781 tgaccaaata tacgcccatc tagtatacca actcctgcta taatagctaa catagtagct
     9841 tgtactaaca tattgaacct ccattttaac ttagtttaat ttacattaaa ttacattaca
     9901 tttttgattt tgttttgatt ttctaataac tcatttacat aaacaattcc attttttgct
     9961 tcctctaatc ctgatttaac tattagtttt gtatcctcct caactaataa aagtatagat
    10021 aaaagcattg gtaaatttac tcctgtcaat aattcaaatt tgaacccttg taaaagtaag
    10081 tttgtacata tgtttgccac acttccacca aaaacatctg ttaagacaac aaattcttca
    10141 ctatctttat tatttgataa tatacttcta atttctttct cagcatcatc tgctgattta
    10201 tccgttgtca tacaaaaata ctcaatatta gttaatgaac caattattaa tttacttgtg
    10261 tcaacaagct ctctggacaa ttctccatgt gttgcaatta gaaatttttt cattctcttt
    10321 ctcctaaatt attttattaa atctgatagt tgtttctttt tgtctctagg taacattcta
    10381 aattctatat caatccctaa atcacctatt tgtttaaagt tatttatatc atcatcatca
    10441 acagcaacta aatctgtaag tttcttcttc ccttcctcat atcttaatcc tcctacattt
    10501 atttctttta atactcctcc tgaattttta gctacaacta aagcatcttt ggtatttttt
    10561 actaacacta atacattgct tttctttgaa gaagcaaatt tttctactat ttctccagat
    10621 tcctctaccc cccttatata taaagttaca ttgctaggtt tcgctaagtt cagtgacata
    10681 gctttcattt ggtcattttt tgcttcatca tttgcaacta gtattacatt tatacctagt
    10741 gatgttgccc atccatatgc aacctgacca tgtatcaatc tgtcatcaat tctcatcaaa
    10801 gataccattt tccccaaatc ctccatcata cattaaaatt tttattgttt atactctcat
    10861 ttatctataa aagcaaaaag tatgccaaat actggcatac ttttttaaat ttcattcact
    10921 tattttgtaa tcttgtattt tactgtggta gaccatagat aatattataa atatatccta
    10981 tctctgaaat tggtatttgc acactataac tcttttctag cacactaaat gcatttttta
    11041 ttagttttac ttcactatga tggtttttga ttaacaaatc catatcagta tactcttcaa
    11101 tttcagtttg tctaactaat ctttcaatca tacaacttaa gtgaacatat aaagaaatct
    11161 ttttatcatt tgaaagtttt ttcttttgaa tattttctat agcattaatt ccttcttcta
    11221 ttctctctat tatctttttt gaatctaaaa tagttagact atctattact ctaattaatg
    11281 aaaaattctt tacaatagaa tcatttatgt tctttatttt ttcattttct actactctgc
    11341 taaatattct aaaaacatca tcttctcctt ttcctgatat tatatcctct aataatataa
    11401 aatctacccc attcacattt ggattggcag ttcctactat agccatcaca tcatacattt
    11461 caaaaactgg ttctttatct cccatagaaa caagactatc ataatcataa ggtattattt
    11521 tgacatcaac tattccaatt aaactttcgg aaagtagatg ttggattttt atagcagtac
    11581 ctatccctgt aaaacaagat gatattatag catattcttt atttttttga ggataataca
    11641 attgtatatt aggtttagtt tcttcatcca taatttctgc aatctcttca atgtttttac
    11701 cttgcattaa taaatttcct acttctagtg cagaaatagt ggatatttgg tctataaata
    11761 acattgggct atttatctta ttctttaaat cttcaaatat aagattaaga gagcccatat
    11821 caaataacac aattaaacca ttctttatcc tatctccctc taggtatcta gacatttgtt
    11881 ttgatacatc agcaatagta gcatctatag gcatatcaat agctgtaaat atatttttac
    11941 ccaacatccg attacataca ttcgccatac tactggcagt agcatatcca tgtgctataa
    12001 tagtcgcatt aacttgatta aatttcattc ttataattgt tgatttaata aatataatca
    12061 ttatgattgt atctacatta ttaagataca tatccaatct attcgtt
//
LOCUS       DAELLV010000108        12090 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.108, whole genome shotgun sequence.
ACCESSION   DAELLV010000108 DAELLV010000000
VERSION     DAELLV010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12090
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.108"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(297..2027)
                     /gene="lpdA"
                     /locus_tag="KPD58_002245"
     CDS             complement(297..2027)
                     /gene="lpdA"
                     /locus_tag="KPD58_002245"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBG0266007.1"
                     /translation="MSVEVIMPKAGVAMEEGTIVSWLKQEGEEVKIGEPILEITTDKV
                     NMEIESEGEGTLAVIIHKEEGEVLPVFTVIGVIAEKGENQEEVKAKYLSGNVSKEDTV
                     KENQNIEVKEEKINKKECNHDYDVVVIGGGPGGYLSALKAALLGGRVALVEENILGGT
                     CLNRGCIPTKTYIKTAEILEEIDQLSKRGVKVTVDKEQDIKKAIKYKNRVVKKLTAGV
                     GGLLKSRDVDVFNLKASVKEEHKVILSDGKVLDTENIIIATGSKVRILPIKGIESNLI
                     ITSTEALDLETVPEELVIIGGGVIGCEFAEIFNSRGSKVTIVEMEDRVIPRMDKELSE
                     SLKYSLSKKGINVLTKKKVSEFKEEGNNILVCIEGEEPIKADLCLYAIGREANLSGIE
                     DLDIKIDKGSIVVNSKMETSMPSIYAVGDVTGGVMLAHAAFKMGEVAASNALGVNKEV
                     DLGALPSCVYTIPEVAAVGITEEDARKKYNVKVGKFNFAGNGRALASGQEQGYVKVVA
                     DAKYGEILGIHMFGCGVAELINHAASFKALEIPTDEASELIFGHPCTSEALMEALADV
                     NGECLHLPKK"
     gene            complement(2194..3240)
                     /locus_tag="KPD58_002246"
     CDS             complement(2194..3240)
                     /locus_tag="KPD58_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HBG0266008.1"
                     /translation="MVANKIKATPAARSQARKDNIKLDRLIGSGENGRIHLVDVLNYL
                     KDNKANTTPLARRIAEDLNIDLETIVGTGYNGKIRKCDVEKLTAKETIVSTNTSKSSE
                     KKELKIENENSSMFNTVEGIFEKPNPMRATVAKRMSESYFSAPVFTFNIEVDATELKV
                     LRAKLIDTVKESTGVKLTMTDLIVMAVSKILPNHQALNSAWTDEGIFRYKDVNIAIAV
                     GLDEGLYVPVVKNANKKSLKEIAKESKELAEKVKTGKLMPADQEGNTFTISNVGMYGI
                     TTFTPIINMPSSAILGVGATQDKFVPVNGEAKIKPIMNLSLTSDHRVIDGTVAAKFLK
                     DLKELLENPLSMLV"
     gene            complement(3253..4239)
                     /locus_tag="KPD58_002247"
     CDS             complement(3253..4239)
                     /locus_tag="KPD58_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HBG0266009.1"
                     /translation="MSTRELTYAQAIKEAMSEEMRRDENVIFMGEDIGIYGGAFGVSV
                     GMIDEFGPERVRDTPISEAAIAGAAAGAAATGLRPIMEVMFMDFVTISMDAIVNQAAK
                     MRYMFGGKAQVPMVVRCPGGSGTGSAEQHSQSLEAWFCHVPGVKVVAPSTPADAKGLL
                     KAAIRDNNPVIFVENKLLYRKKGFVPEDDYVIEIGKADIKREGTDVTVITYGRMLQSV
                     EEAAETLSKENINVEIIDLRTLYPLDKETIVKSVCKTGRVLICHEAAKTGGLGGEISA
                     LITESESFDYLDAPVKRICGKDVPIPYNPELEKAVVPRVDEIEEAIKSLIVR"
     gene            complement(4264..5226)
                     /locus_tag="KPD58_002248"
     CDS             complement(4264..5226)
                     /locus_tag="KPD58_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HBG0266010.1"
                     /translation="MSISKETLLEMYKRMNQARKFEEKVSWFFARGMVHGTTHLSVGQ
                     EASSVAAVMALEKGDLVSLTHRGHSQFIGMGIDLNKMMAELMGKETGFCKGKGGSMHI
                     ADIESGNLGANGVVGGGLTIAPGAALTQQYKKTGKIVLCSFGDGASNEGTFHEGINLS
                     SIWKLPIIFYCENNLYGMSTSIKRHMNIESIATRAASYGIEGISIDGYNPIEVYETVQ
                     KAAEKCRRGEGPVLIESRTYRWLGHSKSDANVYRTKEEIESWKAKDPIEFLKNYLIEN
                     NLSNEDELDKIQEFAKQSIEDAVEFAQNSPNPKIESLLEDVYAD"
     gene            complement(5264..6217)
                     /locus_tag="KPD58_002249"
     CDS             complement(5264..6217)
                     /locus_tag="KPD58_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HBG0266011.1"
                     /translation="MKKIGDLRLMMKCCSLYYEDNLNQQEIANQLGISRPTISRILKE
                     AFEQGIVKIQIVDVLKNDYQKIERSLERKYKLKEVIVVDDKQDALTQKQELARAVSEY
                     LTRVVKENDIIGVSIGTTLKEIPRYVEKSNCKNVTFIPLLGGIGDNEIDIHANQIAVS
                     LARAFGGDFKLLHAPAVMSDLSTKEKLCKDEKIKEVLDLIDKTTIAIVGIGNPMSLNS
                     TIMASGYMNEADIEDLKKYNSIGAICLQAFDKEGKTSILEFNQRVLGVKLEDLKKIKR
                     TIGVASGDEKIEAIKASLKAKFINSLAINHSLALKLLEDCD"
     gene            complement(6662..8035)
                     /locus_tag="KPD58_002250"
     CDS             complement(6662..8035)
                     /locus_tag="KPD58_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0266012.1"
                     /translation="MNTEVNVKATTIKYEKISFFERISYGCGDLGCNIIYSAMSAFLL
                     FYYTNYADVSAAAVGSIMLVSRILDGFSDLTMGIIVDRTKSKYGKARPWILRMAIPFA
                     IAAVLLFSVPSNLGITSKLIYIFITYNLVSTVIYTAINVPYATLNSLITQNQYERGVL
                     SIFRMILATCGTLLINGLTLPLVEYFGNNLSAWTKTFFVFGIASIMVFFITFTGTKER
                     VKAVKQDRNEVIPFKIGIKSLFRNKYWIQITLCLVCIFIVFAINGGSSVYYAKFILGD
                     EKLFGPINMVSNISQIIAMFMVAPFIKKFGKRNVLIVGSIILISSNVMFIIVGQNHIG
                     VIFASAIKGIGSAGIASTMFAIVSDTIEYGEWKTGYRTEGLINSASSFGFKVGNGLGS
                     AILGAVLSIGGYVGTSATQSDLAIVSIKVCFIYLPIFITILQIIIMSFYKLDKEYSTI
                     LNELNTK"
     gene            complement(8132..9778)
                     /locus_tag="KPD58_002251"
     CDS             complement(8132..9778)
                     /locus_tag="KPD58_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBG0266013.1"
                     /translation="MIKNPILPGFNPDPCICRKGDDYYLVVSSFEWFPGIPVYHSKDL
                     KNWELYTHILTDETKIDLKKLPSSKGIWAPCLTYCEEEDLFYIVYGIMNSMNARYFDV
                     DNYLITSKDIKGEWSEPVYLHSSGFDASIFHDDDGKKWIASLDWETREGYEKPGVICL
                     VEYCTKKKEIVGYPKRIWSGGTDRGCIEAPHITKRGDYYYIMCAEGGTGYGHSVTMGR
                     AKNIWGPYEKDSMNPIVTSIPGDFYERHDPDHLKPKYYNPESKLQKSGHGSYIETTSG
                     EVYLVHLTSRPFVPELRCTLGRETAIQKMKWTKDNWLRMEDESNLAKEYVSESKLEEH
                     LVSSIPSFDDFDSNELGLQYYAPRISPLSFADVKSRPGYVRIRGQESRTSLNKVSILA
                     RKLTSVYARITTKMEFYPEVHQHSAGLIMYYDNMNYINLRKYYSETLGQSALSIIHLE
                     NGEKTEFLNTRIPIKDIPIYLRLYIQGRKSYFEWSYDEKNYQRIGKVFDTTKFSDEYC
                     KYGEFTGTFIGLTCADRVKHKHYADFDFFEYIVDESKDVD"
     gene            complement(9800..10966)
                     /locus_tag="KPD58_002252"
     CDS             complement(9800..10966)
                     /locus_tag="KPD58_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HBG0266014.1"
                     /translation="MYIKGVNLGGWLVLEKWMTSSLFEGTEAEDEYYLPRQLSREAYE
                     SRIKTHRSEYITERDFATIKSMGFNSVRIPVPYFIFGDCEPFIGCVKELDKAFAWADK
                     YGLSILIDLHTVPGSQNGFDNGGISGICSWSQNPEYVAFTLNVLERLAKRYGMRHELY
                     GIQILNEPITERMWNIMNVPNRFKAVDKEMARGSKPNSLEFLRDFYIKAYKVMRPYMR
                     EENVIVFHDAFELKAWKDFMREEEFKNVVLDTHQYLMLAEADGCEQSIDSYLKYIREN
                     YAKDILQMQKYFPVICGEWSLFNSYACGIDTNGGQSPLNGIESNIDKLSKDDKRELYR
                     KIAKAQLDAWRNGSGHYYWNYKLLLDTVNEEGWIGWDSWDLGKCVAQEWYPIEY"
     gene            11183..12028
                     /locus_tag="KPD58_002253"
     CDS             11183..12028
                     /locus_tag="KPD58_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0266015.1"
                     /translation="MNRNNFSHEIIEPIDNLDVNFKLFDDSGSYVANHWHNSLEIIYI
                     TSGDLQINMEGYTYNLKANECMFINSGIIHSTRCIYQNTSILLQVPLSFLNKYIPDFK
                     NCYFDFKVNANDNNYKKNSSKVKLILENMREIKLSSPPAANLLFTSLLFELLFELYTN
                     FKISVGNKNMKKTVLDLSKFEPVLEYTKINYKMPISINKIAKVAHLQPEYFCRKFKQY
                     MGQTYLEYLNDVRISHIYKDLINTNDTLCSILEAHGFTNTKLFYRIFKEKFKCTPKHI
                     RKKST"
BASE COUNT         3710 a         2322 c         1290 g         4768 t
ORIGIN      
        1 attaagaaat ttttttttat attttacaaa taatactttt tttaaaaaca aaaaaatcac
       61 ttttcctaat tacaattgaa atcattctaa aatgataatg ttattttata ttactatctt
      121 ttttgtacaa caaaaactac aaggattcta tattctatat ttttccataa aaaatacccc
      181 ttattatgca aataaattat ttgcataata aggggtatgg ctaagtaagt gttttaaaat
      241 tttatgatat aaattaatat taataataga cttaaattaa taatttatgt ttagatttat
      301 tttttaggta agtgtaaaca ttctccattt acatctgcca aagcttccat taaagcttct
      361 gaagtacatg gatgaccaaa tatcaactca cttgcttcat cagtaggtat ctctaacgct
      421 ttaaatgatg ctgcatgatt tataagttct gctacaccac atccaaacat atgtatacct
      481 aaaatttctc catatttagc atctgctact accttcacat atccttgttc ttgaccagaa
      541 gctaaagctc ttccatttcc agcaaaatta aacttaccaa cttttacatt atattttttt
      601 ctagcatcct cttctgtaat accaactgct gctacttctg gaattgtata aacacaacta
      661 ggtaatgcac ctaagtcaac ttctttattc acacctagag catttgaagc tgctacctct
      721 cccattttaa aagcagcatg agctaacatt acacctccag taacatcacc tactgcatat
      781 atgcttggca tacttgtttc cattttacta tttacaacta ttgaaccttt atctatttta
      841 atatctaagt cctctatgcc agaaagatta gcttctcttc ctatagcata tagacataaa
      901 tctgctttta ttggctcttc accttcaatg cacactaata tgttatttcc ttcttcttta
      961 aattcagaaa ctttcttttt agttaataca tttatacctt ttttactaag agaatatttt
     1021 aaagattcgc ttaactcttt atccattctt gggattactc tatcttccat ttctactata
     1081 gttactttag aacctcttga attgaatatc tctgcaaatt cacaaccaat cactccacca
     1141 ccaattatta ctaattcttc tggcactgtt tccaaatcta atgcttctgt actagttatt
     1201 attaaatttg attctattcc ttttataggt aatatcctta ctttggaacc tgtagcaatt
     1261 attatatttt ctgtatctaa aacttttcca tctgataaaa ttactttatg ctcttcttta
     1321 acactagctt taagattaaa cacatctaca tctctacttt ttaataatcc gccaacacca
     1381 gctgtaagtt ttttaactac tctattttta tattttatgg ccttcttaat gtcctgttct
     1441 ttatctacag ttacttttac acctctcttt gataattggt caatttcttc taatatttct
     1501 gctgttttta tataagtctt tgtagggata caacctctat ttaagcaagt acctcctagt
     1561 atattttctt caactaaagc aactcttcca cctaaaagag ctgcttttaa tgctgataaa
     1621 tatcccccag gtcctcctcc tattaccact acatcataat catgattaca ctcttttttg
     1681 tttatttttt cttctttaac ttctatattt tgattctctt taactgtatc ttcttttgaa
     1741 acatttccag ataaatattt agcttttact tcctcttgat tttccccttt ttctgctatt
     1801 acacctatta ctgtaaatac tggtaatact tcaccttcct ctttatgtat gattacagca
     1861 agtgtacctt ctccttctga ctctatctcc atattaactt tatctgttgt tatttctagt
     1921 ataggttctc caatctttac ttcttcgcct tcttgcttta gccaagatac tatagtacct
     1981 tcttccatgg ctactcctgc ttttggcatt attacttcta ctgacatagg ctctcctcct
     2041 atttattttc ttatatttta attaagcaaa tcatattcac atatagttac tgttaaaaat
     2101 aattcttaat atataaaagc tttaaattta ctgaaacaat tataataata taatctattc
     2161 aaactattta tatcagtaac aaaatatcaa aacctataca agcattgata aaggattttc
     2221 taacaattct tttaaatctt ttaagaattt tgcagctact gttccatcta ttactctatg
     2281 gtctgatgtt aatgataagt tcattatagg ttttatttta gcctctccat ttactggaac
     2341 aaacttatct tgtgtagcac ctacccctaa gattgcactt gaaggcatat ttattatagg
     2401 tgtaaatgta gttataccat acattccaac attacttatt gtaaatgtat taccttcttg
     2461 gtctgctggc ataagtttcc ctgtctttac tttttccgca agttctttgc tttcttttgc
     2521 tatttctttt aaagactttt tgtttgcatt ttttactacc ggaacatata agccttcatc
     2581 taaaccaact gcaatagcta tatttacatc tttatatcta aatataccct catctgtcca
     2641 tgctgaattt aaggcttgat gatttggaag tattttagat actgccatta ctattaaatc
     2701 tgtcatagtt aattttactc cagttgattc ttttactgtg tcgattaact ttgctctaag
     2761 taccttcaat tctgtagcat ctacttctat attgaatgta aatacaggag cactaaaata
     2821 actttctgac attcgttttg ctacagtggc tctcattgga tttggctttt caaatattcc
     2881 ttctactgtg ttaaacatgc ttgaattttc attttcaatt ttcagctctt ttttctcact
     2941 agatttagat gtattagttg atacaatagt ttcttttgca gttaattttt ccacatcaca
     3001 ctttcttatc tttccattat atccagtacc tacaatagtt tctaaatcta tatttaaatc
     3061 ttctgctatt cttctagcta aaggagtagt atttgcttta ttatctttta aataatttaa
     3121 cacatctact aaatgaatcc ttccattttc tccacttcca atcagcctat ctaattttat
     3181 attgtctttt cttgcctgac ttctagctgc tggtgtggct tttattttat ttgccaccat
     3241 atctatccct ccttatctaa caattaaaga ttttattgct tcttctattt catcaactct
     3301 tgggacaact gctttttcta actctggatt atatggtatt ggcacatctt ttccacaaat
     3361 tctttttact ggagcatcta aataatcaaa tgattcactt tctgttatca gggctgatat
     3421 ttctccacct agcccaccag tcttagctgc ttcatggcaa attaaaactc tcccagtttt
     3481 acatacactc tttactatag tctctttatc taatggatat aaagttctta aatctataat
     3541 ttcaacattt atattttctt ttgatagagt ctctgctgct tcttcaacac tttgtaacat
     3601 tcttccataa gtaattactg taacatcagt accctctctt ttaatatctg cctttccaat
     3661 ttcaattaca taatcatctt ctggcacaaa accttttttt ctgtataaca atttattctc
     3721 aacaaatatg actggattat tatctcttat agctgctttc aacaatccct tagcatctgc
     3781 tggtgtagaa ggtgcaacta cttttactcc tggtacatga caaaaccaag cttctaaact
     3841 ttgtgaatgt tgctctgctg aaccagttcc agaacctcct ggacatctaa caaccattgg
     3901 aacttgtgct tttcctccaa acatatatct catttttgcc gcttgattta caattgcatc
     3961 catagaaatt gttacaaagt ccataaacat tacttccata ataggtctta agccagttgc
     4021 tgctgcacct gctgccgctc ctgctatagc tgcttctgat ataggagtat cccttactct
     4081 ttctggtcca aattcatcta tcataccaac tgatacacca aatgctcctc catatattcc
     4141 tatatcttct cccataaata ttacattttc atctcttctc atttcttctg acattgcttc
     4201 ctttattgct tgtgcatatg ttaattccct tgtactcatt tttattttcc tcctttacca
     4261 ttcttagtct gcatatacat cctctaataa agactctatt tttggatttg ggctattttg
     4321 agcaaactct actgcatctt ctattgattg ctttgcaaat tcttgaatct tatctaattc
     4381 atcttcatta gatagattat tttctataag ataatttttt aaaaattcaa tagggtcttt
     4441 tgctttccat gattctattt cttcttttgt tctatataca tttgcatctg attttgaatg
     4501 acctaaccat ctataagttc tactttcaat taatacaggt ccttctcctc ttctacactt
     4561 ctcagcagct ttttgtactg tttcgtatac ttctattggg ttatatccat ctattgatat
     4621 tccttctatt ccataagaag ctgctctagt ggcaatactt tctatattca tatgacgttt
     4681 tatgcttgtt gacataccgt ataaattatt ctcacagtaa aatattattg gcaatttcca
     4741 tattgaagat aaatttattc cttcatgaaa agtaccttca tttgatgctc catcaccaaa
     4801 actacataaa actattttgc ctgttttttt atactgttgt gtaagtgcag ctcctggagc
     4861 tattgtaaga cctccaccta caactccatt tgctcctaag ttacctgact caatatctgc
     4921 tatatgcatt gagcctcctt ttcccttaca aaatccagtt tctttaccca taagttcagc
     4981 catcatttta tttaggtcta ttcccattcc tataaattga ctatgtcctc tatgagttaa
     5041 tgatactaaa tctccttttt ctaaagccat tacagccgcc acacttgacg cttcttgacc
     5101 tacagataag tgagttgttc catgaaccat acctcttgca aaaaaccaac taactttctc
     5161 ttcaaatttt ctagcctgat tcattctctt atacatttca agcaatgttt cttttgatat
     5221 tgacatatac attccctcct gttaactttt atctatcttt ctattagtca caatcttcta
     5281 ataatttaag tgctaaactg tgattaattg ctaaactatt tataaatttc gcttttaatg
     5341 atgcctttat tgcctctatt ttttcatctc ctgaagcgac acctattgtt ctttttatct
     5401 tttttaaatc ttccagtttc actcctaata ctctttgatt aaattcaagt atagaagttt
     5461 ttccttcttt atcaaaggct tgtaaacaaa ttgcacctat tgagttgtat ttttttaaat
     5521 cttctatatc tgcttcattc atgtatccag aagccattat tgttgagttt aaactcatag
     5581 gatttcctat acccacaatt gctatagttg ttttatctat taaatctagt acttctttaa
     5641 ttttttcgtc tttgcaaagt ttctcttttg tagacaagtc agacataaca gctggagcat
     5701 gtaataattt aaaatcacct ccaaaagccc tcgctaaact tactgctatt tgatttgcat
     5761 gaatatctat ttcattgtct ccaatccctc ctaatagagg tataaatgta acatttttac
     5821 agttactttt ttccacatat cttggtattt cttttagtgt tgttcctatt gaaacaccta
     5881 ttatatcatt ttcctttact actctagtta aatattcaga tacagctctt gctagttctt
     5941 gtttttgtgt tagtgcatcc tgtttatcat ctactactat tacttctttt aatttatatt
     6001 ttctctccaa acttctctct attttttggt aatcattttt caaaacatca actatctgta
     6061 tttttactat tccttgttca aaagcttctt ttaatattct tgatattgtt ggtcttgata
     6121 ttcccaattg atttgctatt tcctgttgat tcaaattatc ttcgtaataa agtgaacaac
     6181 acttcatcat taatcttaag tctccaattt ttttcatttg ctttcctcca aaatcaagct
     6241 tttcaattat ctttacattt gttcaatcat tttacttaat ttcagtaaca ttatagaagg
     6301 ggttcatata aaaagtcaac tgtttttttg tataacaaaa aaaaggtgat acttttcttg
     6361 taccaccacc ttcataaata gttgaaaaat tgcttcttag tgcatattat atattacttc
     6421 taattgttaa atatcatctc cataaattaa aatttaattg tccacttata atgctttaaa
     6481 taacaataac ttattaaatc atttctaaaa aaccaaccat ttcatcattt aaagtcttct
     6541 tatattttta ttattcgctt ctctaaaaat tcttcaataa tcataaatat ataatagttt
     6601 ttatagggaa catttacaat agaccttaat taaacttaag actttataag aaactttttc
     6661 attatttagt atttaattca ttaagtatag tactatattc tttatcaagc ttataaaaag
     6721 acattatgat aatttgtaat attgttataa atataggtag gtatataaaa catactttta
     6781 ttgatactat agctaaatca ctttgagtag ctgatgttcc tacataacca ccaattgata
     6841 atactgcacc taatattgct gaacctaatc catttcccac tttaaaccca aaactagatg
     6901 cactatttat aagcccctca gttctatatc cagtcttcca ttctccatat tcaatagtat
     6961 ctgaaacaat agcaaacatt gttgatgcta ttccagcact tcctattccc tttatagcac
     7021 ttgcaaatat tactcctata tgattttgtc caactattat aaacataaca tttgacgata
     7081 ttaatattat tgaacctact attaatacat ttctttttcc aaattttttt ataaaaggag
     7141 caaccataaa catagctata atttgggata tatttgaaac catatttata ggaccaaata
     7201 atttttcatc tcccaaaata aactttgcat aatatacgga tgaaccacca tttatcgcaa
     7261 atacaataaa tatacaaact aaacataatg ttatttgtat ccagtactta tttctaaata
     7321 aagatttaat acctatttta aatggtatta cttcattcct atcttgctta actgctttta
     7381 ctctttcctt ggttccagta aatgttataa aaaataccat tatagatgct attccaaata
     7441 caaagaatgt tttagtccat gctgataaat tatttccaaa gtattcaact agaggtagtg
     7501 taagtccatt tattagtaag gtaccacaag tggctaatat cattctaaat atacttaata
     7561 ctcctctttc atactggttt tgagttataa gtgaatttag agttgcatag ggtacattga
     7621 ttgctgtata aataacagta gaaactaagt tatatgtaat aaaaatataa attaatttag
     7681 aagtgatacc aagatttgat ggaacagaaa ataataatac tgctgctata gcaaaaggaa
     7741 tcgccattct caaaatccaa ggcctagcct taccgtattt ggatttagtt ctatccacta
     7801 taattcccat tgttaagtca ctaaacccat ccagtatcct agatacaagc ataatactac
     7861 ctacagctgc tgcactaaca tctgcataat tagtataata aaacagcaaa aatgctgaca
     7921 tagctgagta tatgatattg caccccaaat ccccacaacc ataagaaatt ctttcaaaaa
     7981 atgaaatctt ttcatattta atcgtagttg ctttaacatt aacttctgta ttcataatac
     8041 catactcctt tttgtaagat ttgccttcta atgtttctta tttcagagca taagatttac
     8101 ctttgctctg aaaattttag taatgatttt attaatcgac atcttttgat tcatctacta
     8161 tatattcaaa aaaatcaaaa tcagcatagt gtttatgctt tacacgatct gcacaagtta
     8221 atcctataaa cgtacctgta aattctccat atttacagta ctcatctgaa aacttagttg
     8281 tatcaaatac ctttcctatt ctctggtagt ttttttcatc ataactccat tcaaaatatg
     8341 acttacgacc ttgtatatac agacgaagat atattggtat atcttttatt ggtatcctag
     8401 tatttaaaaa ttccgttttc tctccatttt caagatgtat tattgatagt gcactttgac
     8461 ccaatgtttc actataatac tttcttaaat ttatataatt catattatca taatacatta
     8521 tcaaacccgc actatgctga tgtacctctg ggtaaaactc catcttagtg gttattcttg
     8581 catatactga agttaatttt cttgccaata tgctcacttt atttaatgat gttcttgatt
     8641 cttgtcctct aatccttaca tatcctggtc tagacttaac atctgcaaat gataatggtg
     8701 aaatcctagg agcatagtac tgtaaaccta attcatttga atcaaagtca tcaaaacttg
     8761 gtatagatga aactaaatgt tcttctagtt tactttctga tacgtattct tttgccaaat
     8821 tactttcatc ttccattcga agccaattat cctttgtcca tttcattttt tgaatagctg
     8881 tttctctacc taaagtacac cttaactctg gtacaaatgg tctagatgtt agatgaacta
     8941 aatatacttc acctgatgtt gtttctatat agcttccatg accagatttt tgaagttttg
     9001 attctggatt atagtattta ggctttagat ggtcagggtc atgtctttca taaaaatcgc
     9061 ctggaattga agttacaatt ggattcattg aatctttttc ataaggtccc catatatttt
     9121 tagcccttcc catagttaca ctatgaccat atccagttcc accttctgca cacattatat
     9181 aatagtaatc tccacgcttt gttatatgtg gtgcttcaat acaacctcta tcagttcccc
     9241 ctgaccatat tctctttgga tatcctacta tttctttctt ttttgtacag tattccacta
     9301 aacatataac accaggtttt tcatatcctt ctctagtctc ccagtctaat gatgcaatcc
     9361 actttttacc atcatcatca tgaaaaatag acgcatcaaa acctgatgag tgtagatata
     9421 ctggttcact ccactctccc tttatatctt ttgatgtaat taaataatta tcaacatcaa
     9481 aatatcttgc attcatagag ttcataattc cataaactat gtaaaataaa tcttcttctt
     9541 cacaataagt caaacaaggt gcccaaattc ctttagatga aggaagcttc ttcaaatcta
     9601 tttttgtttc atctgtcaaa atatgagtat ataactccca attttttaag tctttagagt
     9661 gataaacagg tatacctgga aaccattcaa aagatgaaac tacaagataa taatcatctc
     9721 cctttctaca aatacaagga tctggattaa aacctggaag tatagggttt ttaatcatat
     9781 cttttcctcc aattttagtt taatattcta taggatacca ttcttgagct acacatttgc
     9841 ctaggtccca actatcccat cctatccaac cttcttcatt aactgtatct agtaataact
     9901 tataattcca atagtagtga ccacttccat ttctccatgc atcaagctgt gcttttgcta
     9961 tcttcctata taattctctt ttatcgtctt ttgataattt atctatattt gattcaatcc
    10021 catttagagg tgattgacct ccatttgtat ctataccaca agcatatgaa ttaaataagc
    10081 tccactcccc acatataaca ggaaaatatt tttgcatttg taaaatatct ttagcataat
    10141 tttctctaat atatttgaga tagctatcaa tactttgttc acatccatct gcttcagcta
    10201 acatcaaata ttggtgtgta tctagaacaa catttttaaa ttcttcctct ctcataaagt
    10261 ctttccatgc ttttaattca aatgcatcat gaaatactat aacgttttcc tctctcatat
    10321 aaggacgcat taccttatat gcttttatat aaaaatcacg taaaaactct aatgaattag
    10381 gtttactccc ccttgccatc tccttatcta cagccttaaa ccgatttgga acattcatta
    10441 tgttccacat tctttctgtt ataggctcat ttaaaatttg gattccatac aattcatgcc
    10501 tcatcccata acgttttgca agtctttcta atacatttaa tgtaaacgct acatattctg
    10561 gattttgtga ccagctacat atacctgata ttcctccatt atcaaatcca ttttggcttc
    10621 ctggaactgt atgtaagtct atcaatatac taagcccata cttatctgcc caagcaaatg
    10681 ctttatcaag ttctttaaca catcctatga atggttcaca atctccaaaa ataaagtatg
    10741 gtactggtat acgaactgaa ttaaatccca tagatttaat cgttgcaaaa tctctctctg
    10801 tgatgtattc actacgatga gtcttaattc tagattcata tgcctctctt gaaagctgtc
    10861 ttggtaaata gtattcatct tcagcttctg tgccttcaaa taaacttgag gtcatccatt
    10921 tttctaaaac taaccaacct cctaaattta caccttttat atacacataa aatcctcccc
    10981 tcttatctta tttaaatagg tatattttgc acttataata aaataatttt tttaagttat
    11041 tttaatatac agttattata tttgatttta tctgttttct taagtatgtt tattatctaa
    11101 tttattagtc tatattatct ttttaatatt gaacattata aatttactat ttataattta
    11161 aaattataag gaggatatta ttatgaacag aaataatttt tcacatgaaa taattgaacc
    11221 tattgataat ttagatgtta atttcaaact atttgatgat agtggtagtt atgtagcaaa
    11281 tcattggcat aactcattag aaataattta tataacttct ggtgatttac aaattaacat
    11341 ggagggttac acttataatt taaaagctaa tgagtgtatg ttcattaatt ctggaattat
    11401 tcactcaact agatgtattt atcaaaatac ctctatatta ttgcaagtac cattgtcttt
    11461 tctaaacaaa tatatccctg attttaaaaa ttgctatttt gactttaagg ttaatgccaa
    11521 tgacaataac tataaaaaaa attcaagtaa agttaaactt atattagaaa acatgagaga
    11581 aattaaactt agttctcctc ctgcagctaa tctgctcttt acaagcttat tatttgaact
    11641 tttatttgaa ttatatacaa actttaaaat atctgtaggt aataaaaata tgaaaaaaac
    11701 agtacttgat ttgtcaaaat ttgaaccagt tttagaatat acaaagatta attacaaaat
    11761 gcctatttct attaacaaaa tcgctaaggt agcacactta caaccagaat atttttgtag
    11821 aaaatttaaa cagtatatgg gacaaactta cttagagtat ttaaatgatg tgcgtatttc
    11881 tcatatttat aaagatttaa ttaacacaaa tgatacctta tgcagtatat tagaagctca
    11941 tggatttact aatacaaaat tattttacag aatttttaag gaaaaattta aatgcactcc
    12001 taaacatata agaaaaaaat ctacctaaaa tatgaggtta tatgttattt atctttataa
    12061 taaaaaaagt ttatcttaaa atttgattag
//
LOCUS       DAELLV010000109        12048 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.109, whole genome shotgun sequence.
ACCESSION   DAELLV010000109 DAELLV010000000
VERSION     DAELLV010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12048
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.109"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..827)
                     /locus_tag="KPD58_002254"
     CDS             complement(<1..827)
                     /locus_tag="KPD58_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBG0266016.1"
                     /translation="MRERIILETLKNSSGYLNIDYFADKLGVSTRTIRNEIKKIETIK
                     ECNGFKLEYKSKLGYILNIKDQDKFEHYLRNLPSDLVENPEQRLESIIVELLVNEGYK
                     TIEQLSKKFLVSSSQIKNDLKKIDEKIKDTELKLERKAHYGIKIEGSIKEIQSILVDS
                     YFRGNRNITEYRNKFIDNLKLANIRSTIKNVLNEHDLEANLTELEEILAQIIILYIRV
                     SMRVVGNASDLKLYTEDLIIDKLLDKIFRDKKYNLNYDEKYYLKELIKLKTKDKKAT"
     gene            complement(1153..2004)
                     /locus_tag="KPD58_002255"
     CDS             complement(1153..2004)
                     /locus_tag="KPD58_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBG0266017.1"
                     /translation="MALVTTTSMLKKAQDEGYAVGAFNVENMEMVIAVIEAAEELNSP
                     VILQTTPSTVKYAGLDYYLANAKVAAERVNIPVAMHLDHGSSFDLAMKALRSGYTSIM
                     IDGSHESFENNISISKSVVKACSPSLIPVEAELGKVGGKEDDLDGGNSNDYTNPKEAK
                     EFVDRTGISSLAIAIGTAHGLYKGEPKIDLDRLSEIKNVVSIPLVLHGGSGIHDDIIK
                     EAIKRGIAKVNYATELRIAYSKGVKKVLDEDSEVIDPKKYGLAGLQSVKDFVKEKIKV
                     CGSVNRV"
     gene            complement(2053..3222)
                     /locus_tag="KPD58_002256"
     CDS             complement(2053..3222)
                     /locus_tag="KPD58_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG0266018.1"
                     /translation="MKMFKLEEQKLKDLGAIITTNEIKQQPELWLETYEIYKSNKEKL
                     SRFIDTISNNHGQFRVIFTGAGTSAYIGNSILPYLKNKNDIRKYIFEAIPTTDIVSNP
                     YDYLKKDIPTLLISFARSGNSPESLAALNLGNKIVDNFYHLAITCNPEGELAKMTKND
                     ENNYLLLMPSKSNDEGFAMTGSFSCMMLSAMLIFDSLEDDVEKSYINAIIEMGRNVID
                     RKDEIHELINKDFDRVVYLGSGGLGGLTQEAQLKLLELTAGKISTVYDSPMGFRHGPK
                     SFIDENTLVFEFVSNCLYTRKYDLDVLEEIKRDKIAKFTCAVSVENENNFSGTKFEFK
                     EKYNKLPDVYLAMPYILFAQTIALFVSVKVGNKPDTPSATGTVNRVVKGVTIYEY"
     gene            3693..4610
                     /locus_tag="KPD58_002257"
     CDS             3693..4610
                     /locus_tag="KPD58_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBG0266019.1"
                     /translation="MKNIKMGMIGLGSIAQKAYLPILTKSERFEFVGAFTPNKVKREK
                     VCSDYRIMPFDSIESLAKKCDCIFLHSSTETHYEIIKILLNLGVHVYVDKPLASTVSQ
                     GEELIELSTKKNLNLMVGFNRRFCPMYKEIKNNATEIVSINICKHGLNSLRNVRFDST
                     LIDDYIHVIDTALWLANEDVEISGEDLFLTDNKNLIFVSHKLKGKNFSINTSMHRDSG
                     TKLEQVEILSKGKIQRVKNLNVLEIEEGGNLTLKQSGAWVNILKQKGFEDISNHFIDC
                     IENNIKPAINGEECIKAQRLLEKIINSVK"
     gene            complement(4739..5668)
                     /gene="pfkB"
                     /locus_tag="KPD58_002258"
     CDS             complement(4739..5668)
                     /gene="pfkB"
                     /locus_tag="KPD58_002258"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBG0266020.1"
                     /translation="MITVITFNPSIDRMYRVNNINIGEVQRVVSKNATAGGKGLNVAK
                     VCKILKENPLAMGFLGGFNGEFIKNELRKLDIQNKFTKIEQETRNCLNIIEDNKVSTE
                     FLEKGPIVEKSDLERFENDIEEVLKNTKILVASGSYCENMPIDYYEKIGNTCRANNIK
                     FILDTSGEALKIALKSKPYLIKPNIDEIRHLLGMNIESREEIILSGKKLIEMGAENVC
                     ISLGKDGMVYLNEDNVYDVKVPVVKCINTVGSGDSTVAGFSVGILRGYKIENLLKFAN
                     ACGISNALNMETGFVDLGEIDKYQDLVEVKKLS"
     gene            5935..6660
                     /locus_tag="KPD58_002259"
     CDS             5935..6660
                     /locus_tag="KPD58_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0266021.1"
                     /translation="MIKRNDKRPIYDQLVEILRTKIENEMEPNDRMLSERKICDEYGV
                     SRTTVRLAMAELEHMGYIYKRHGKGTFVAALSQNSQNLMESYSFTDYMRTQGKKPGTK
                     VLSFEVVESTKYFSEKLGITTGEKMIKIMRLRLADDLPMMLERTYLPMKEFIGLTKEK
                     IEQKPLYEIFREDYGEIIKVADEEFSAGIISDKEAKLLEVPGDSPCLKLLRTTYNDDN
                     IVIEFTLSVARSDKFVYRVRHVR"
     gene            6743..7897
                     /gene="nagA"
                     /locus_tag="KPD58_002260"
     CDS             6743..7897
                     /gene="nagA"
                     /locus_tag="KPD58_002260"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBG0266022.1"
                     /translation="MKSFIYADKFFMKHGIKEKGYLSIINGKFGEFQQERPNNTDNVI
                     DFSGKYIAPGLVDTHIHGLLGADVMDNTFEAINTISKGLLKYGVTSFLPTTLTDSVDT
                     LSDSVENISNSFNRVEGAKIQGIYLEGPFFTEKYKGAQNEKYFKDPDINILKLWQKKS
                     KNLIRKIAIAPERKGSIELTRYATKNNISVSLGHSSATFEQVKQVVNSGAKVFVHTYN
                     GMSPLNHREPGMVGAAMVLKDTYAELICDGHHVSPIAVKIMMDAKSSNNIALITDCMR
                     AGAMEDGKYTLGKFNVNVKNSVARLNSGSLAGSVLTMDKAVRNIVDWNISTLEDAIKM
                     ATYIPALSCNIDNVCGSIYQGMDADFIVTDNDFNIHETYINGICMYKVNK"
     gene            complement(8080..9237)
                     /locus_tag="KPD58_002261"
     CDS             complement(8080..9237)
                     /locus_tag="KPD58_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HBG0266023.1"
                     /translation="MYLVISKKKAIFLGVILVIITAMVTSAFQLTLGNKVVISKELYE
                     DYKKYDKLLGLESIIKQDFYKKVSDTDLVNGAAKGLFLGTNDKYSGYYTKDEMENLIN
                     DSEGSYVGVGMYIGASKDGGLVVVPMKDSPAEKAGVKSGDKLVKVNGKSVSYKNSDEA
                     VRMMKGKKGKTVELTILREDKQLNFKVKTDQIIEKSIESKVIDNDLGYIEITQFISST
                     YTDFDKALKELKAKNIKGLVIDLRNNPGGMLDICKEVADELIGEGTIVYTKDNKGNTE
                     YLKSDKEKLGLPIVVLTNGESASAAEILTAAIVDNKEGISVGTTTFGKGLVQSVVRLK
                     DGTGYKLTTAQYFTPNGDYINEKGIKPTIEEKDENKQLDVATKWLREQIDK"
     gene            9391..9960
                     /locus_tag="KPD58_002262"
     CDS             9391..9960
                     /locus_tag="KPD58_002262"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBG0266024.1"
                     /translation="MKKEFRKKVIESRKNKSANFVLSNSNLITEKLLQMEDIRNATNI
                     MLYLNFNNEVQTDNLIKKLLSLKKIVSSPITIKENHTLIPTQITDLKEGLRIGAYGIR
                     EPNEKSPTIDIKALDVVIVPAVAYDIHCYRLGYGGGFYDRFLENLRKDAITIGIAFDF
                     QIFDSIPKETHDAQLDYIVTETRIITPNS"
     gene            complement(10142..10558)
                     /locus_tag="KPD58_002263"
     CDS             complement(10142..10558)
                     /locus_tag="KPD58_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266025.1"
                     /translation="MNVKFNIKGIIYGVIALVFVIGGFIFIPSMFVEKSKPIDYTVLQ
                     RNSIPEKILDVMDKYTNEERALAAKIDNKIYIIVTRGNNKYGIEMDKIESVVEEGKDV
                     LRVKIKYKDKEDSFPYIVVETNMSELPDRIELNATK"
     gene            complement(10784..11878)
                     /locus_tag="KPD58_002264"
     CDS             complement(10784..11878)
                     /locus_tag="KPD58_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266026.1"
                     /translation="MSEKVNVKTVISFAGAYVATVIGSGFATGQEIMQFFSFYGFAGI
                     IGGIISMVLFSWMGASVMSKGKELQLKEPIKIYRVYCGKYLGIFFEWFGPLFLFGVFV
                     VMISGAGATLTEYYGLNPFVGRVGMAVVALLTVSLGLDKLSKILGGIGPIIIIFTLLV
                     GGISLAKNIGNIGEAAQVLSTVKIAKPVPNAYLSGVIYTTYNTIVVMAFLTGLGASAA
                     NKKEAICGGILGGVALMAAAIMMHLAILSDIGNLYSKAIPSLFLADKISPVIGVLFSI
                     ILILGIYTTAVPLLWSVTNRFVDDKHPKFKLITLIAAILGLIGGFLPFDKLVGILYPY
                     TGYMGVIILVCVLYRQITKTSGYQEGISDK"
BASE COUNT         4155 a         1936 c         1421 g         4536 t
ORIGIN      
        1 atagtggctt tcttatcttt agtctttagt tttataagct cctttaaata atacttttca
       61 tcataattaa ggttatattt tttatctcta aaaattttat ctaataattt atctataatt
      121 aaatcttctg tatataattt taaatcgcta gcatttccca ctactctcat actaactctt
      181 atatacaata taataatttg tgctaatatt tcctcaagtt ctgttaaatt tgcttctaaa
      241 tcatgttcat taagtacatt ttttattgta cttctaatat tagctaattt taaattatct
      301 ataaacttat tcctgtactc tgttatattt ctattccctc taaaataact atccaccaat
      361 atactttgaa tttcttttat agaaccttct attttaattc cataatgagc ctttcgctct
      421 agttttaatt ctgtatcctt tatcttttca tctatctttt ttaaatcatt ttttatttgg
      481 gatgaactta ctagaaattt tttacttaac tgttctatag ttttataacc ttcatttact
      541 aaaagctcta ctattataga ctccaaccgt tgttctggat tttcaactaa atctgagggt
      601 aagtttctta gataatgctc gaatttatct tggtctttaa tatttagaat atatcctaat
      661 ttagacttat attctaattt aaaaccatta cattctttta tagtttctat cttttttatt
      721 tcatttctga tagttcgagt agataccccc aacttatctg caaaataatc tatgtttaga
      781 taaccactac tattttttaa tgtttcaaga ataatgcgtt ctctcatatc cccacctccc
      841 tcagtgttat tattgtgttt tttaaagtgg tataaaaaat attttttata aaaacaacct
      901 tttcttttta tttaagaatt ttatatccta gtatatttta attccacaat ttaatataaa
      961 tattttataa taataaaata tatagttcaa tttttagtat attttaatta tatatgtaat
     1021 actttaccaa taaaagctga tatttttcat caattaaatg aaaaaaatac accatttaca
     1081 tataattaat ataaattttt catataataa atatcctaaa cttatctaaa ataaatttag
     1141 gatattttat ttttatactc tatttacaga cccacacact ttaatctttt ctttcacaaa
     1201 atcctttact gattgaagcc ctgctaatcc atacttttta gggtctataa cctcagaatc
     1261 ttcatctaaa acttttttaa cccctttact atatgcaatt cttaattcag ttgcatagtt
     1321 aacttttgca atacctcttt ttatagcttc ttttataata tcatcatgta ttcctgaacc
     1381 tccatgcaat actaaaggta ttgaaactac atttttaatt tctgataacc tatctaaatc
     1441 tattttaggt tcacccttat acaatccatg tgcagtacct atagcaatag ccaatgaaga
     1501 tattcctgtt ctatcgacaa actcttttgc ctcttttgga tttgtataat cattactatt
     1561 cccgccatct aaatcatctt cttttccacc aactttccca agttctgctt ccactggtat
     1621 aagagaagga ctacatgctt ttactactga tttagatata gatatattat tttcaaagct
     1681 ttcatgagaa ccatctatca ttattgatgt atatccactt cttaaagctt tcattgcaag
     1741 gtcaaaactt gaaccatggt ccagatgcat tgctacaggt atatttactc tttctgcagc
     1801 aacctttgca tttgctagat aataatcaag acctgcatat tttacagtag atggagtagt
     1861 ttgtaatatt actggagaat ttaactcctc agcagcttct ataacagcaa ttaccatttc
     1921 catattttct acattaaatg ctcctactgc atacccttca tcttgtgcct ttttaagcat
     1981 tgatgttgtt gttactaatg ccacattgat accctcccaa attatttagt tataatattt
     2041 taatcttatc ttttaatatt catatattgt tacacctttt actactcgat ttactgttcc
     2101 tgtagctgat ggagtatctg gcttatttcc aacctttact gatacaaaca atgctatagt
     2161 ttgtgcaaac aatatgtatg gcattgcaag atacacatct ggtaatttat tatacttttc
     2221 tttaaactca aactttgttc ctgagaagtt attttcattt tctactgaca ctgcacaagt
     2281 gaatttagca attttatccc tttttatctc ctctaaaaca tctaaatcat attttcttgt
     2341 atataaacag ttagatacaa attcaaatac aagtgtattt tcatcaataa atgattttgg
     2401 tccgtgtcta aaccccatag gagaatcata cactgtagat atttttcctg ctgtaagttc
     2461 taaaagtttt agttgtgctt cttgtgtcaa accacctaat cctcctgaac ctaaatatac
     2521 aactctatca aaatctttat ttattaactc atgtatttca tcttttctat ctataacatt
     2581 tcttcccatt tcaattattg catttatata tgacttttct acatcatctt ctaaactatc
     2641 gaatattaac atagcagata acatcataca cgaaaaactt cctgtcatgg caaagccttc
     2701 atcatttgat tttgatggca ttaataacaa gtaattattt tcgtcgtttt tagtcatctt
     2761 agccagttct ccttctggat tacaagttat agctaaatga taaaaattat ctacaatctt
     2821 attccctaaa tttaaagctg ctaaactttc aggactattt ccacttcttg caaatgaaat
     2881 taataatgtt ggtatatctt tttttaagta atcatatggg tttgaaacta tatccgtagt
     2941 aggtattgct tcaaatatgt attttcttat gtcattttta tttttaaggt aaggaagtat
     3001 tgaatttcct atgtaagctg aagttccagc acctgtaaat ataactctaa actgcccatg
     3061 attattactt attgtatcta tgaatctact taatttttct ttgttagatt tatatatttc
     3121 ataagtttct aaccataatt caggctgttg ttttatttca ttagttgtta ttattgcacc
     3181 taaatctttt agtttttgtt cttctaattt aaacattttc attctccttt catacttgta
     3241 acgtttacat ttggtaatga ggttataacc actttaaact ataaaaaaat atactctttt
     3301 ataatttttt gcttagattt tttagtacta cttaaactca ctataaaata tacactatag
     3361 attttaatta attttatagt ttatctttaa aaatttaata aaatcatact ctatatactt
     3421 tagtattttt attttacgat aaaagcttgt tcagaccaaa gtggtaatta ccactaatcc
     3481 aaatataaat taatttgaat tttttgtcaa tacataaaat atttatatca atcaatttta
     3541 tggatatcct acaaaattac tttatcaata tttcttattt cgctaattta cttatataag
     3601 ctttaaattt attttttgat acaaagttta caaatttatg tgtctgaatt ataatcaatt
     3661 ctgtaaatat aaaaacctta ggagaaagta ttatgaaaaa tataaaaatg ggaatgatag
     3721 gtcttggctc catagctcaa aaggcatatt tacctatatt aactaagtct gagagatttg
     3781 agtttgtagg agcttttact ccaaataaag ttaaaaggga aaaagtctgt agtgattata
     3841 gaatcatgcc attcgattct attgaatccc tagctaaaaa atgtgattgt atttttctac
     3901 atagctctac agaaacacac tacgaaatta taaaaatatt attaaattta ggtgttcatg
     3961 tatatgtaga caaacctctt gcttcaacag taagtcaagg agaagaactt atcgagttaa
     4021 gtacgaaaaa aaacttaaat ttaatggttg gattcaaccg tagattctgt cctatgtaca
     4081 aagaaattaa aaataatgct acagaaattg tatctatcaa tatatgtaaa catggattaa
     4141 attccttaag aaatgttaga ttcgacagta ctcttataga tgactatata catgttattg
     4201 acactgcttt atggttagca aatgaagatg ttgaaattag tggagaggat ttatttttaa
     4261 cagacaataa gaatcttata tttgtaagtc ataaacttaa aggtaaaaat ttctctataa
     4321 acacctctat gcatagagat tctggaacaa agttagaaca agttgaaatt ctttcaaagg
     4381 gtaagattca aagagttaag aatcttaatg tactggaaat agaagaaggt ggaaacctaa
     4441 ctttaaagca aagtggtgca tgggtaaata tattaaaaca aaagggtttt gaggatatat
     4501 caaatcattt tattgattgt atagaaaata atataaaacc agctattaat ggagaagagt
     4561 gtatcaaagc tcaaagatta ctagagaaaa ttataaattc agtaaaatag atatttatta
     4621 taaaaatagg ttaaaaatga atttttctaa cctattttta tataagacta tatcttttat
     4681 attaaaatta aaatctatta caacaacatt aaatttatta tattcccatt aaatgtattt
     4741 aacttaactt ttttacttca actaaatctt gatatttatc aatttctccc aaatctacaa
     4801 atccagtttc catatttaaa gcattactta taccacaagc atttgcaaat ttaagtaaat
     4861 tttctatttt ataacccctt aaaataccta cactaaatcc agccactgtg ctatctccac
     4921 tacctacagt atttatacac tttacaactg gtacttttac atcatataca ttatcttcgt
     4981 ttaaatacac cataccatct tttcctaaag atatacatac attctctgca cccatttcaa
     5041 taagtttttt tcctgacaag ataatttcct ctcttgattc tatattcatt cctaataaat
     5101 gtcttatttc atctatattt ggttttatta aatatggttt tgattttaaa gctattttta
     5161 atgcttctcc tgatgtatct aatataaact ttatattatt tgctctacat gtatttccta
     5221 tcttttcata atagtctatc ggcatattct cacaataact accagaagct accagtattt
     5281 tagtattttt tagtacttcc tctatatcat tttcaaatct ctctaaatcg cttttttcta
     5341 ctattggtcc tttttctaaa aattctgtac taactttatt gtcctcaatt atgtttaagc
     5401 aatttctagt ttcctgctct atttttgtaa atttattttg tatatctagt tttctaagtt
     5461 cattttttat aaattctcca ttaaatccgc ctagaaatcc catagctaaa ggattttctt
     5521 ttaaaatttt acatacctta gctacgttta atcctttacc tccagcagtt gcattcttac
     5581 ttactaccct ttgcacctca cctatgttta tattattcac tctatacatt ctatctatag
     5641 atgggttgaa tgttattact gtaatcatac tagattctcc tcattattaa tttttacttt
     5701 tctaaacaat aaattaatct gtaaaataaa aaatacctca tataatatat ccaaagtggt
     5761 tattaccaat ttgaatataa attattttac ttgttttgtc aatgtaccat aattttataa
     5821 taaattaata gccttactta ttgaattttg attaaacttt attcattgac ataaataatt
     5881 ctatattcta taatcaaatt gtggtaataa ccacttagaa aggactatat ttttatgata
     5941 aaaagaaatg ataaaagacc aatatatgac cagcttgttg agatattaag aactaagatt
     6001 gaaaatgaga tggaaccaaa cgatagaatg ctttccgaaa gaaaaatttg tgatgaatat
     6061 ggagtaagta gaaccacagt tagactagct atggctgagt tggaacatat gggatatata
     6121 tacaaaagac atggaaaagg aacttttgtt gctgcactaa gtcaaaattc tcaaaactta
     6181 atggaaagtt atagttttac agattatatg agaacacaag gaaaaaagcc aggtacaaag
     6241 gtattatcat ttgaggtagt agaaagtaca aagtatttct ctgaaaaatt aggtataact
     6301 actggtgaaa aaatgataaa aataatgcgt ttaagacttg ctgatgacct tcctatgatg
     6361 cttgaaagaa catatcttcc tatgaaagaa tttataggat tgacaaaaga aaaaatcgaa
     6421 caaaaacctt tatatgaaat atttagagaa gattatggcg agattattaa agttgctgat
     6481 gaagaatttt cagctggaat aatctctgat aaggaagcta agttattgga agtaccagga
     6541 gattcacctt gtctgaaatt acttcgtacc acatataatg atgacaatat agttattgaa
     6601 tttactttga gtgtcgctag aagcgataaa tttgtatata gagtaagaca tgtaagatag
     6661 taagttaaat agctttcaag aaaacagaat gatattataa gcgtcaatgc cacaatgata
     6721 gtataattat aaggaggtta ttttgaaaag ttttatttat gcagataaat tttttatgaa
     6781 acatggaatc aaagaaaaag gttaccttag tataattaat ggcaaattcg gtgaatttca
     6841 acaagaaaga cctaataata ctgataatgt tatagatttt tcaggtaaat atattgctcc
     6901 tggcttagtt gatactcaca ttcacggtct tttaggtgca gatgtcatgg acaatacatt
     6961 tgaagcaata aacacaattt caaaaggctt attaaaatat ggagtaacat ctttcctacc
     7021 tacaacttta actgattctg tagatacact aagtgattct gtagaaaata taagcaatag
     7081 cttcaatcga gttgagggtg caaaaataca agggatttat ttagaaggtc cattttttac
     7141 agaaaaatat aagggcgctc aaaatgaaaa atattttaaa gaccctgaca tcaatatatt
     7201 aaaattatgg caaaaaaaat cgaaaaattt aataaggaaa atagcaattg ctccagaacg
     7261 taaaggttca atagaattaa caagatatgc aactaaaaat aatatatcag tttctcttgg
     7321 tcatagttca gctacatttg agcaagttaa acaagtcgtt aattcaggtg ctaaagtgtt
     7381 tgtacataca tataatggga tgagtccttt aaatcataga gaacctggaa tggttggggc
     7441 tgctatggta ctaaaagata cttatgctga attaatttgt gatggtcatc atgtaagccc
     7501 aatagctgtt aaaataatga tggatgctaa gtctagtaat aacatagctt taattacaga
     7561 ttgtatgaga gctggtgcta tggaggatgg aaaatatact cttggtaagt ttaatgtaaa
     7621 tgtaaaaaat agtgttgcaa gattaaatag tgggagttta gctggaagtg tattaactat
     7681 ggacaaggct gtgagaaata tagttgattg gaacatatca actttagaag atgcaataaa
     7741 aatggctaca tatatccctg ctttaagttg taatattgat aatgtctgtg gctctatata
     7801 tcaaggtatg gatgcagact ttatagtaac agataatgac tttaatatac acgaaactta
     7861 tataaatggt atctgtatgt acaaagttaa taaatagatt caatataaag ttatagaggc
     7921 ttcctaaaaa tagagataag cctctttttc attgttaaaa tgatgaaaat acatttgatt
     7981 tcatacaaaa agagtgcctc atgaactaaa aagttcattt tgagacatct cttttataat
     8041 gttctttatt ttatagtata agtttataaa aattaaatcc tatttatcaa tctgttctct
     8101 aagccactta gttgctacat ctaactgttt attttcatct ttttcttcaa tagtaggctt
     8161 tatacctttt tcattgatat aatctccatt aggtgtaaaa tattgagcag tagttaactt
     8221 gtatcctgtt ccatccttca atcttacaac tgattgtacc aaccctttac caaaagttgt
     8281 agtccctaca gaaatacctt ctttattatc aacgattgca gctgttaata tttcagcagc
     8341 agaagcactt tctccattag ttaacactac tattggaagt cccaattttt ctttatcaga
     8401 tttcaagtac tcagtatttc ccttattatc ttttgtatat actatggttc cttcaccaat
     8461 taactcatca gcaacttctt tacatatatc tagcattcca ccaggattat ttctaaggtc
     8521 tattactaat cccttgatat tttttgcttt taattctttt aaagctttat caaagtcagt
     8581 atatgtactt gatataaatt gggtaatttc tatatatcct aaatcattat caattacttt
     8641 actttctata ctcttctcaa ttatctggtc tgtttttact ttaaagttca attgtttatc
     8701 ttctcttaat atagtcagtt caactgtttt acctttttta cccttcatca tacgaacagc
     8761 ttcatctgaa tttttatatg agacagattt tccattgact tttactaatt tatcccctga
     8821 ttttacacct gctttctctg ctggagaatc tttcatggga actactacta agccaccatc
     8881 ttttgatgcc cctatataca tacctacacc aacataagaa ccttcactat cattaattag
     8941 attctccatt tcatctttag tataataacc agaatactta tcatttgttc ctagaaatag
     9001 accttttgct gctccattta ccaaatcagt atctgatact tttttataaa aatcttgttt
     9061 tataatagat tctagtccta acaacttatc atatttttta tagtcttcat agagttcttt
     9121 cgatattaca actttattcc ccaatgtcaa ctgaaatgct gatgtaacca tagctgttat
     9181 aattactaaa atcaccccta aaaaaatagc tttcttcttt gaaatcacaa gatacatctc
     9241 cttttacata aactattatt attatatgac tagctgttat ttttattaca tttacccaaa
     9301 tatattgctt tattacaata aattatacta aaatttatat taaatacaat tgctgtacac
     9361 attataacat attaagggag gaattatttt atgaaaaaag aatttagaaa aaaagttatt
     9421 gaatcaagaa aaaataagag tgcaaacttt gtacttagta attcaaattt aataactgaa
     9481 aaattacttc aaatggaaga tataagaaat gcaactaata taatgcttta tctaaatttt
     9541 aacaatgaag ttcaaacaga taatttaatc aaaaaacttt tgtctctaaa gaaaatagta
     9601 tcaagtccca tcacaataaa agaaaatcat acattaatcc ctactcaaat aacagattta
     9661 aaagagggat tgagaattgg agcatatgga ataagagaac ctaacgaaaa atcacctaca
     9721 attgatatta aggctttaga tgttgtaatt gtaccagctg tagcatatga tatacattgc
     9781 tatagattag gatatggtgg aggattttat gatagatttt tagaaaactt aagaaaagat
     9841 gctataacta tcggcatagc atttgacttc cagatattcg attctattcc aaaagaaact
     9901 catgatgcac aattagacta catagttact gaaactagaa ttatcactcc aaatagttag
     9961 aatattaagt atttccagac cccatttaat gtagtaataa atactactgt taatatctta
    10021 ctttataagc attttaagtc atggctttta caacaataag aaccatacac aatttactta
    10081 cattaataaa aaccatgact ttattgtaca atatgcagtt atttaattat aaaatacctt
    10141 cttatttcgt cgcatttaat tctattctat ctggaagttc actcatattt gtttcaacaa
    10201 ctatgtatgg aaaagaatct tccttatctt tatactttat cttgactctc aaaacatcct
    10261 ttccttcctc aaccacactt tctattttgt ccatttctat accatattta ttatttcctc
    10321 tagtcactat aatgtatatt ttgttatcga ttttagccgc taatgctctt tcttcatttg
    10381 tatacttatc catcacatct aatatctttt ctggtattga atttctttgt aatactgtat
    10441 aatctatggg tttagatttc tctacaaaca tacttggtat gaatataaat ccccctataa
    10501 cgaatacaag agctataact ccataaatta tacctttaat attaaacttt acattcaatc
    10561 ttatcactcc tcccatatat tctaaatata tgatttataa acttatctta gactctaaat
    10621 tattgattag tatatattta tacataatta taaatatatg taatgtaact aaaaaacatg
    10681 ttttttctat ttcataataa acgagctaaa aaataaaaag agctatatga aaacattttc
    10741 attgttttca tatagctctt gagtttacaa tattaaaaaa tacttatttg tcactgattc
    10801 cttcttgata tccagatgtt tttgtaatct gtctataaag aacacaaacc agtattatga
    10861 cgcccatata tccagtatat ggatataaaa taccaaccag cttatcaaat ggtaggaagc
    10921 cacctataag acctaatata gctgctatta aagttattaa tttaaactta ggatgtttat
    10981 catctacaaa tctattagtt actgaccata ataaaggtac agctgttgta tatataccta
    11041 atataagaat tattgagaat aaaactccaa ttactggtga aatcttatct gctaagaata
    11101 atgatggtat tgcttttgaa tataagtttc ctatatcaga aagaatagct aagtgcatca
    11161 ttatagctgc agccataagt gctactccac ctaatatacc accacaaata gcttcttttt
    11221 tgttagcagc acttgctcct aatccagtta agaatgccat aacaacaatt gtgttataag
    11281 tagtatatat tacaccagat agataggcat ttggaactgg tttagctatc tttacagtgc
    11341 taagaacttg tgcagcttct cctatattac ctatgttttt agctaaactt ataccaccaa
    11401 ctaaaagtgt aaagattatt attataggcc ctattccacc tagtatcttt gataatttat
    11461 caagacctag tgaaacagtt aataatgcta ctactgccat acctactcta cctacaaatg
    11521 gatttaatcc atagtattca gttaaagttg ctcctgctcc tgaaatcatt actacgaaaa
    11581 caccaaataa gaataaaggt ccaaaccatt caaagaatat tcctaaatac ttaccacaat
    11641 aaacacgata aattttgata ggttctttaa gttgaagctc tttacctttt gacataactg
    11701 aagcacccat ccatgagaat aataccattg aaattatccc accaataata cctgcaaaac
    11761 cataaaaaga gaagaattgc attatttcct gtcctgttgc aaatccagaa ccgattactg
    11821 ttgctacata cgcacctgcg aaactgatga ctgttttaac atttactttt tctgacatat
    11881 ctcagttacc tccccatgtg aaattttttc ttctgattaa acaattcgat aaactaccta
    11941 caatttcctt taacataaaa tacatgataa ctgttttata gctttttagt ataataactt
    12001 aaaacttata atctgatttt ataccctact cctcctctca taattttt
//
LOCUS       DAELLV010000110        11947 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.110, whole genome shotgun sequence.
ACCESSION   DAELLV010000110 DAELLV010000000
VERSION     DAELLV010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11947
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.110"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            266..1444
                     /locus_tag="KPD58_002265"
     CDS             266..1444
                     /locus_tag="KPD58_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG0266027.1"
                     /translation="MKYHIFLIITTLLIIELCNIYICKIASFGNKNITIHKNYKYIFL
                     IKPLTFLIIIFTKLPLPLYYILFYIVSHKLLLSRKSSTNNHFIPNMAVISFCILHLIT
                     VSTISLVKNTSPDIIFANMTYKLISILIASILDFILWYSMNKIKEHIYIITQNQIEFS
                     LFLKFIWFTLGYILFDSIQITFAPPSSLTSYFILGSNILLILQLIIFINHIYSIEKNI
                     YLEKEHHRLKMIKQEQLLKVNLLKKKANIDELTSAYNRKFAFEKIQHYIDNNLLFSLI
                     YIDLDKLKTINDTYGHLAGDNYIINFSSIVIENLGIDDIFARIGGDEFIVIMPKSNEN
                     TTKEFIEKISKIMDNKISFSYGIVEVSSENKMTVEEIINIADIRMYTNKQNNHKRNNN
                     "
     gene            1794..2675
                     /locus_tag="KPD58_002266"
     CDS             1794..2675
                     /locus_tag="KPD58_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG0266028.1"
                     /translation="MPAYLFDSNSITNIYRVFSILLSFIVTGLYFSYKNHKNNKINFE
                     AVLEDKKNLIFLLSLMFGMYLYLCLSLSIYYTVGNVLILKLYGIKSAIIVLVGNYIAE
                     RYSFRITQIKGYDIKNQIEYVESVKTEYEINKLNDIVYTDLLTGFYNRPFVNQKLSEW
                     TSNHYKFTLCFTDLNSLKPVNDKFGHQFGDKYISTTAQIIKKLINVENSLLFRYGGDE
                     FLFLLQNTSCVEAEKIMININYELSKLSNTDEYPYALSISYGLVEWDGISDIETLISK
                     ADSLMYENKLKNKSTLN"
     gene            2884..3033
                     /locus_tag="KPD58_002267"
     CDS             2884..3033
                     /locus_tag="KPD58_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008819224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG0266029.1"
                     /translation="MTTVAEGIETSFQKDFLQEIDCDMLQGYVFSRPLPIKDFEKLMF
                     QNSSN"
     gene            complement(3293..5860)
                     /locus_tag="KPD58_002268"
     CDS             complement(3293..5860)
                     /locus_tag="KPD58_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HBG0266030.1"
                     /translation="MIYYVLPLEHKQATIEIVGGKGMSLSKLLTAGIPVPDGFHVTTA
                     SYQIFVETNHIQSRINKLLDGIDSNNTSQLEDVSKKIGELFHNGEMPQEVSDAIKTAY
                     AGLGNISVAVRSSATAEDLPDASFAGQQETYLNIQGEDKVIDAVKRCWASLWTARAIA
                     YRVKNNIKHEIVALAVVVQKLAFSDSSGIMFTLNPINGRRSEMIINAAWGLGEAVVSS
                     LVTPDTIVVDKDSERIISYEVANKEIMTVRTSEGTEETMVPERLRKKYALTRNQVMQL
                     IQLGKKIEKYYQMPMDVEWALEKDKLYIVQARPITVLPPEWVLPEQDVVYTKGSLAEH
                     LPNPVTPLFATLGLEIVNRASELLWIDMFGKSAKKLLPKNGAYTVINGYVYLSAKSKP
                     LLIAIKSLSPRSLRRTLMNSVARWETARKGYEDAIKQWEEKPLHTMNAHQIMEGIQTI
                     FYAACIYFTRIQLTLPAASISETLFTKLFQGAVRRAGITDTSVFLLGFDTIVLQSEKA
                     LWGISEWVKQNNSINLYLQNSSAIKIAEDFMSSVVPVGVSQEVWIEWKNRINKYFKEF
                     GRTAYEFDFAHSTPQETLTPTFESVKTFVEGKGESPFLRQTTFEKRRKHAEEEILQHI
                     GGLRKKLFFKLLHWAQETSPMRENAIYLMGMGHPLIRCMFQKISERFIRGGAISHLDD
                     IYWLTKSELEVLIEQLDKNKSLSDMNGIIPVRKAELKKYMGYVSPSKLPEKNKKTISQ
                     EPQKQKDGKIVLTGIGTSTGVVTAPACVLNSPADFESFQPGSVLVAVTTTPAWTPLFS
                     SASAIVTDIGGPLSHSSIVAREYGIPAVMATHIATRTIKSGQMITVDGLAGTVTFDE"
     gene            complement(5863..6468)
                     /locus_tag="KPD58_002269"
     CDS             complement(5863..6468)
                     /locus_tag="KPD58_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0266031.1"
                     /translation="MPKGIVLTPEQQAERREKIVAVALSLMAENGFQKTSMREIAVLA
                     NMGKSSLYDFFKTKDEIVVYAVEKEIEKTIQQVHRIIDGEPSPQQCLRKIMLNHLRFP
                     EQYRTVLMWLNTESDYLEEEYRKRLKDARYAYQDIIKSVIDNGVTTGIFRKTDTALVT
                     RLLINSMLSISYTSRPSDSSEKMLDETMNIFLHGIMNDGGE"
     gene            complement(6842..8302)
                     /locus_tag="KPD58_002270"
     CDS             complement(6842..8302)
                     /locus_tag="KPD58_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HBG0266032.1"
                     /translation="MFNELKTKENVYKNLINGKWVESNSRKPIEIYSPIDNSLVGKVQ
                     SMTKHEVDEVIKNTKESIKVWAEMPVYKRANIFHKAADLLLENIDEIANVLVLEIAKD
                     IKSARAEVERTADFLRYTADVGKNMEGEAISGDNFPGGTRNKMSYVSRVPLGTVLAIS
                     PFNYPVNLSMSKIAPALIGGNAVVLKPATQGAISALHVVEIMRKAGIPDGVLNTITGR
                     GSEIGDYVVTHKGINFINFTGSTEVGQHISKISGMVPLLLELGGKDAAIVLEDADLDF
                     AAKNIVSGAYSYSGQRCTAVKRILVQESVADKLVGKIKPLVEKLTIGNPMDEVVITPL
                     IDNKATDFVQGLVDDALHKGAKLITGNVRKNNLFYPTLLDNVNVDMKIAWEEPFGPVL
                     PIIRVKDINQAIEIANQSEYGLQSSVFTSDIDKAFYIADKLEVGTVQINNKTERGPDH
                     FPFLGVKASGMGTQGVKYSIEAMTRPKAVVVNVREL"
     gene            8657..9235
                     /locus_tag="KPD58_002271"
     CDS             8657..9235
                     /locus_tag="KPD58_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0266033.1"
                     /translation="MAKSFTEIEKDNIRKRLISECELNWSKYGYKKTNIDELCSKSGI
                     AKGSFYTFFDSKEKLFFEVLNGIQNRLISSVDEILSNMPPKEGFAKSLKMLYRLYDKN
                     PFLYSPNNPDFISLINKLPQDMFLQLESNNIQSFYDLIERHNLKLKIEKEKVFGVCNA
                     LLSSLLVKENIEYNYIEVFDFMVDNLVEHILE"
     gene            9526..10776
                     /locus_tag="KPD58_002272"
     CDS             9526..10776
                     /locus_tag="KPD58_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0266034.1"
                     /translation="MVKSSNTFNKNFNLMILGQIISLFGASILKFALSLYILDITGKA
                     EIFATILAVSSIPIIVFSPIGGAIADIFNRRNLMVIFDFSSSLTVLILAVFLFNNNGS
                     ILLVGIIMTILSVISTMYQPTVQSSTPLLVDNEHLMNANGIVAGVASLTNIAGPVLGG
                     VLYGVIGINAIIVISCVSFFLSAIMEIFIQIPFIKQEQNSNIIKTIFTDIKDGIVHIC
                     KENRFILKILFLAIAINLFLSSMLIVGIPYIVKITMGASNFLYGLAEGAISFSSLVAA
                     VSIGIFSKHLKITNLHISFIICAVMFIPMAVSVYPFILNTGFPVPFLIFIVSSMLIFF
                     TTTLISIYVITVTQLKTPNELLGKVMSILFAGTAIAMPLGQVMYGKLFDLLSDRVYII
                     ILVVGFITLLIAFVSKITLRNENL"
     gene            10937..11506
                     /locus_tag="KPD58_002273"
     CDS             10937..11506
                     /locus_tag="KPD58_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBG0266035.1"
                     /translation="MSYMPTLEQAWEILRKYNKEEFHIRHAQIVSGVMRYYAKEYDPE
                     RVEFWEIVGLLHDLDFEMYPDEHCVKQRELMTELDLDKSIIDSTISHGYGLTGSDVKP
                     EQFMEKVLFAVDELTGLIGAAAIMRPSKSVSDLELKSVKKKFKDKSFAAGCSRDVIRS
                     GAEMLGWELDKLIQTTIEAMRSLIPEMAI"
BASE COUNT         4134 a         1968 c         1650 g         4195 t
ORIGIN      
        1 catgctatat gtaattacta tacttataca cataactatt atacttacca tacataatta
       61 ctatatttgg acacatagtt atcatacata ctgtatatta agatataata ctttctaaat
      121 tttatataac acagacacat gcaataaaaa agaaggtatc cataataagc tctttgcatg
      181 tacctaaata aaaactgtaa aatagaatat ataaaatcta atctatttta agtaaataca
      241 atttcacttt aaaagaggat gtactatgaa ataccatata tttttaatca taactacttt
      301 attaataatc gaactatgta acatttacat atgcaaaata gcatcttttg gaaacaaaaa
      361 tataactata cataagaatt acaaatatat ttttttaata aaaccattaa ccttcttgat
      421 aataatattc acaaaattac cattaccact atattatata ttgttttaca tagtaagtca
      481 taaattgctc ttaagcagaa aaagttctac taacaaccat tttattccca atatggcagt
      541 aataagtttt tgtattttac accttattac tgtaagtaca atatcacttg ttaaaaatac
      601 aagtccagat ataatatttg caaatatgac ctataagtta attagtattt taatagcttc
      661 tattttagat tttatcttat ggtactctat gaataaaata aaagagcata tttatataat
      721 aactcaaaat caaatagagt tcagtctatt tttaaaattt atctggttca ctttaggata
      781 tatattattt gatagtatac aaattacatt tgctccacca agttctctaa cttcctactt
      841 tatattggga agtaacatat tattaatctt acaactaatt atattcatca atcatatata
      901 ttccattgaa aaaaatattt atttagaaaa agaacatcat agactaaaaa tgataaaaca
      961 agaacaatta ctaaaagtaa atttattaaa gaaaaaagct aacattgatg aattaacatc
     1021 agcatataat cgaaaatttg cttttgaaaa aatacagcat tacattgata acaatttatt
     1081 attttcattg atatatatcg atttggataa attaaagacg ataaatgata catatggaca
     1141 tttagctggg gataactata ttattaactt ctcaagtata gttattgaaa acttaggaat
     1201 agatgacata tttgctagaa taggaggaga tgagtttata gttattatgc ctaaatctaa
     1261 tgaaaacacc acaaaagaat ttatagagaa aataagtaaa attatggata ataagatatc
     1321 ttttagctat ggtattgtag aagtttctag cgaaaacaaa atgactgtag aagaaattat
     1381 taatattgct gatattagaa tgtacactaa taaacaaaat aatcataaaa ggaacaataa
     1441 ctaaaatgaa tatttacatg agcttacttg tagtctcact atgtttttta tcttattatc
     1501 tctatcatta taattttttt aaatatctag acataatgat taataactct aaaaataaat
     1561 acaaagtata tattgtatac tctcttttta attatttaat ttttattatt tgtacttatt
     1621 taaggttaca cctagttata aattggacaa tttttatgtt aattttattt atgcaaatta
     1681 aagtaactta caaactttcc acaataaaaa acctttttta ttcgttaaac tgctccatca
     1741 ttggtttagc aataaatata ttttttaggt ctttaatgtc aatagtgtta aatatgccag
     1801 catatttatt tgatagtaat tctattacaa atatttacag agtattttct atattgctta
     1861 gttttatagt tacaggatta tatttcagtt ataaaaatca taaaaataat aagattaact
     1921 ttgaggcagt tttagaggac aaaaaaaact taattttctt attgagcctt atgtttggaa
     1981 tgtacttgta tctatgtttg agtttgtcaa tatactatac tgttggaaat gtattgattt
     2041 taaaattata tggaatcaag tctgccataa tagttttggt aggtaactat attgcagaaa
     2101 gatatagctt tagaattact caaataaaag gttatgatat taaaaatcaa atagaatatg
     2161 tagaaagtgt aaagacagag tatgaaatta ataaattgaa tgatattgta tatacagatt
     2221 tactgacagg cttctacaat agaccttttg taaatcaaaa actatcagag tggacatcaa
     2281 atcactacaa atttacctta tgttttactg atttaaatag cttaaaacct gtaaatgata
     2341 aatttggaca ccaatttggt gataaataca tatccactac tgctcaaata ataaaaaaac
     2401 ttataaatgt agaaaattcc ttactattta gatatggtgg agatgaattt ttattccttt
     2461 tacagaatac aagttgtgta gaagcagaaa aaataatgat taatataaat tatgaattat
     2521 caaaattaag taatactgac gagtatcctt atgctctttc cataagctat ggattagttg
     2581 agtgggatgg aatatccgat attgaaactt taatatcaaa ggcagattct ttgatgtatg
     2641 aaaataagct taaaaataaa tctactttaa actagctagt taagatattc tacaaaatat
     2701 atgttataag agagtactaa gaatgtcaaa atatatagat tttgcaaata ttgaatacag
     2761 tttactaatg aatgcactgg atgtaagtgt tagtaaacac ctattagatg aacactttac
     2821 tgttgtttgg gctaatgacc gttattatga aatgttcggc tatacctagc taaaaagtta
     2881 aaaatgacca ctgtagccga gggaatagag accagttttc aaaaagattt cttgcaagaa
     2941 atagactgtg atatgttaca aggatacgtg ttctctagac ctttaccaat aaaagacttt
     3001 gaaaaactta tgtttcaaaa ctcctccaat taacaatata aaattgctta ttataagtga
     3061 tattattgta tcaacaaata aaggaattgt ctcaaaattg attttttcta atcaaatttt
     3121 tattttgaga cattctcatt tttttgcata agaaaatacc caccaagcta cggtttttct
     3181 taccgctgtt cggtgggtat tagtagtatt atgaaatctg aaaactttaa gtattactac
     3241 ttccgctaac gcaatcacaa aggtcgaaag cttcaaagcg aaaataggct ttttactcat
     3301 caaacgtcac cgtacctgcc aacccgtcca ccgttatcat ttgtccgctc ttaatagttc
     3361 gtgtggcaat gtgtgtagcc ataacggctg ggatgccata ttcacgtgcg acaatgcttg
     3421 aatggctaag aggaccacca atgtccgtta caatcgcact tgcagatgaa aataatggtg
     3481 tccatgctgg agttgtggta actgcaacca aaacacttcc aggttgaaag ctttcaaaat
     3541 ctgctggact attcagtaca caagctggag ccgtcacaac accagtactg gtaccaatgc
     3601 ccgtcaacac aattttccca tctttctgct tctgtggttc ttgtgatata gtttttttgt
     3661 ttttctctgg caacttggat ggtgacacat aacccatgta ttttttaagt tcagcttttc
     3721 gaacaggtat tatcccgttc atatcagata gagacttgtt tttatccaac tgctctataa
     3781 gtacctccaa ttccgatttt gtgagccaat aaatatcatc taaatgtgaa atagctcctc
     3841 ctcgtatgaa tcgttctgag attttctgaa acatacaacg aatcagagga tgtcccattc
     3901 ccatcaaata aatagcattt tcacgcatag gagaggtttc ctgtgcccaa tggagcaact
     3961 taaaaaatag ttttttacgc aatccaccta tgtgttgtaa aatctcttct tctgcatgtt
     4021 ttctacgctt ttcaaacgtg gtctggcgta aaaaaggact ttcccctttt ccttccacaa
     4081 atgtttttac ggattcaaaa gttggtgtaa gtgtttcctg cggtgtggaa tgcgcaaaat
     4141 caaactcata agcggtacga ccaaactctt taaagtactt attgattcga tttttccatt
     4201 ctatccatac ttcctgtgaa actccaacag gcacaactga agacatgaaa tcttctgcta
     4261 tctttattgc agaactgttt tgcagataaa ggttgatgct gttattttgc tttacccatt
     4321 ctgaaatacc ccacaacgcc ttttccgatt gcagaacaat cgtatcaaac ccaagcagga
     4381 aaacggaagt atccgttata ccagcacgtc gaactgctcc ttgaaataat tttgtaaata
     4441 atgtttcact gatagaagca gcaggcaatg taagctgaat ccttgtgaaa taaatacatg
     4501 cggcataaaa tattgtctga ataccctcca taatttgatg ggcgttcata gtatgcaggg
     4561 gcttttcttc ccattgttta atagcatcct catatccttt tcgtgccgtt tcccagcgtg
     4621 caacgctgtt cataagtgtc ctacgtaaag agcgtgggga aagggatttg atagcaatca
     4681 aaagtggctt tgatttagca gaaagataaa catatccatt aataaccgta tatgccccgt
     4741 tttttggtaa taattttttc gcacttttac caaacatatc tatccataaa agctctgatg
     4801 cacggtttac gatttcaaga ccaagcgtgg caaacaaagg tgtaacagga tttggcaagt
     4861 gttctgccaa acttcccttt gtgtatacaa catcctgttc tggcaatacc cattctggtg
     4921 gcagaacagt aattggacga gcctgaacaa tgtacaattt atctttttcc agtgcccact
     4981 ctacatccat aggcatttga taatacttct caattttctt tccaagctgt atcagttgca
     5041 ttacctgatt acgagtaaga gcatattttt ttctcaacct ttcaggaacc atggtttctt
     5101 ctgttccttc tgaagtacga accgtcataa tctctttatt tgccacttca tacgatataa
     5161 ttcgttcgga atccttatcc actacgattg tatcgggtgt aactaagcta gaaaccaccg
     5221 cttcaccaag cccccatgca gcgttaataa tcatttcgct acgtctacca ttgattgggt
     5281 ttaacgtaaa cataatacca gatgaatcgg aaaatgcaag cttttgtact acaacagcaa
     5341 gtgcgacaat ctcgtgcttt atattattct tcacacgata agcaatagca cgagctgtcc
     5401 ataaggaagc ccagcatctc tttacagcgt caataacttt atcttcacct tgtatgttaa
     5461 gataagtttc ctgctgacct gcaaaagaag cgtcaggtaa atcttcagca gtcgcagacg
     5521 aacggacagc caccgatatg ttccctaatc cggcataagc cgttttaatt gcatccgaca
     5581 cttcctgtgg catttctcca ttatggaaaa gctcccctat tttcttagat acatcttcaa
     5641 gttggctggt attattggaa tcaataccat ctagcagttt attaattcga gactgaatat
     5701 ggttcgtctc aacaaaaatc tggtatgaag cagtcgtaac atgaaaaccg tctggcacag
     5761 ggatacctgc tgtcaatagt tttgataaag acatcccttt tccaccaaca atttcaatgg
     5821 ttgcttgttt atgttccagt ggcaagacat aataaatcat aatcattcac ctccatcatt
     5881 cataattccg tgcagaaata tattcattgt ttcatccaac attttttccg aactatccga
     5941 tggtcgtgac gtgtagctga ttgataacat ggagttaatt aacaaacgtg tcacaagagc
     6001 ggtatccgtt ttgcgaaaga tgcctgtggt cacaccatta tcaattaccg attttataat
     6061 atcttgatat gcataacgag catctttaag tctctttcga tactcttctt ctaaataatc
     6121 agattctgta tttaaccaca tcagtacagt tctatactgc tctggaaatc taagatgatt
     6181 aagcatgatt tttctaaggc actgttgtgg tgaaggttca ccatcaataa tcctatgaac
     6241 ttgttggata gttttttcta tttctttctc aactgcatat acaacaatct cgtctttcgt
     6301 tttgaaaaaa tcgtatagac ttgactttcc catgttggct aaaactgcaa tttctctcat
     6361 tgatgttttt tggaatccat tttcagccat gagactcaaa gcaacagcaa ctattttttc
     6421 tcggcgttct gcttgttgtt cgggtgtaag cacaattcct tttggcatag ggcgatacct
     6481 ccttttagcg accactggtc gtttcttttt tatttatcat acagcgacca acggtcactt
     6541 gtcaaggtta atttttttca ttgtatatgg tactcgagat aaaaatgttc attttagaat
     6601 ttgctctatt taagtagaaa taggcttcat cattagaaaa caagactata aatttgagat
     6661 atttatcatg ttcaaatttc atcaatatca aagtttaaga tattaataaa aaaagaatta
     6721 ctaagaaata acatataaag attcaaaaaa ggagttgaac ttaaaattta attacaaaaa
     6781 atcaaattta agacaactcc tttattcttt atctctacta ttctttatct ctatttattt
     6841 attacaactc cctaacattt accactactg ccttaggtct agtcatagcc tcgattgaat
     6901 acttaacacc ttgagttccc ataccagatg cctttacacc taaaaatggg aagtggtctg
     6961 gacctctttc agtcttatta tttatttgaa cagtaccaac ttcaagttta tcagctatat
     7021 aaaatgcctt atcaatatca cttgtaaata ctgatgattg aagcccatac tctgattgat
     7081 tagcaatttc aattgcttga tttatatctt taactcttat aattggaagt actggaccaa
     7141 atggttcttc ccaagcaatt ttcatatcaa catttacatt atctaacaat gttggataga
     7201 ataaattatt ttttctaaca ttgcctgtta ttaactttgc tcctttatgt aatgcatcat
     7261 caaccaaccc ttgaacaaaa tcagttgctt tattatctat aagaggagtg ataactactt
     7321 catccattgg atttccaatt gtcaacttct ctaccaatgg ctttattttt ccgaccaatt
     7381 tatccgccac actctcttgt actaaaattc tctttactgc cgtacatctt tgtcctgaat
     7441 atgaatacgc tccagatact atattcttag ctgcaaaatc taagtctgca tcttctaata
     7501 ctatagccgc atcttttcca ccaagctcca ataataatgg taccattcca gatattttag
     7561 atatatgctg accaacttca gtactaccag taaagttaat aaaattaatt cctttatgcg
     7621 ttacaacata atctcctatc tcactacccc tacctgtaat agtattaagt actccatctg
     7681 gtatcccagc ttttctcata atttctacca catgaagtgc gctaatagcc ccttgtgtag
     7741 caggttttaa tacaactgca tttccaccaa taagtgctgg agcaatcttt gacattgata
     7801 aatttacagg ataattaaaa ggagatatag ctaaaacagt acctaatgga acccttgaaa
     7861 cataagacat cttatttctt gttccaccag ggaaattatc tccactaatg gcttcaccct
     7921 ccatattttt tcccacatca gctgtgtatc ttaaaaaatc tgctgttctc tcaacttctg
     7981 cccttgcaga ttttatgtct ttggctattt caagtaccag tacattagct atctcatcta
     8041 tattttctaa caataaatca gcagccttat gaaatatatt ggctctcttg tatacaggca
     8101 tttcagccca tacttttata ctctcttttg tgttttttat aacctcatct acctcatgtt
     8161 ttgtcataga ttgtactttc ccaacaagag aattatctat tggtgagtaa atttctattg
     8221 gctttctcga attagattct acccattttc catttattaa atttttatat acattttctt
     8281 ttgttttaag ttcattaaac atatttttat tcctctctct gatataactt tgtgtattta
     8341 aattatagcc atttattcgt tatgtaaaca tttctttttt tcataaacgt ttacctataa
     8401 aaaaaatttt aagttaacgt ttttctacat tcttctattt taataacata ctcgctccaa
     8461 atctatatag gtcacacttt aatatcaata tactaatata ccaatatacc aatataccaa
     8521 tataccaata taccaatata aatattataa acataaatca cttgacaaat ttataagtag
     8581 acaatattat atatctatga ctcaaaatat ttttaaagtc atatatatca tatatagata
     8641 gaaaggattg ataaacatgg ctaaaagttt tacagaaata gaaaaagaca atataagaaa
     8701 aagactaatt tcagaatgtg aactcaactg gtctaaatat ggatacaaaa aaactaatat
     8761 tgatgaactt tgttcaaaat caggaattgc aaagggttct ttctatacat tttttgattc
     8821 aaaagaaaag ttattttttg aagtattaaa tggaatccaa aacagactta tatcatcagt
     8881 agatgaaatt ttaagtaaca tgcctccaaa agaaggcttt gcaaaatcct taaaaatgtt
     8941 atatcgatta tatgataaaa atccattttt atattcacca aataatcctg atttcatcag
     9001 cctaataaat aaactacctc aagatatgtt tttacaactt gagtccaata acatacaaag
     9061 tttttacgac ttaattgaga gacacaatct caagttgaag atagaaaaag aaaaagtttt
     9121 tggtgtttgt aatgctctac tatcaagtct attggtaaaa gaaaatattg aatataacta
     9181 tattgaagtg tttgacttta tggtggataa tcttgtagaa cacatacttg aataacaatt
     9241 taaaataatt tttctaagta tttttttata ctaaatagga ttaatatgat tgaattaata
     9301 caaaaaataa acaatataat ttataaaata aatgataaac taaatatcac acaaaactta
     9361 tagaccctag agcaaaccca ttccactcta ctctaagcca tctataatag tttgtatatt
     9421 tctaataata ttagatatta aagtaagaaa aaaattcctt ataataattg ctattatatc
     9481 aattattata aatattttaa taatcttaga taggtaggta catatatggt aaaatcctcg
     9541 aacactttta ataaaaactt caacttgatg atattaggtc aaataatctc attgtttggt
     9601 gcatcaatac ttaaatttgc tttatcttta tacatacttg atataactgg taaagcagaa
     9661 atatttgcta ctatactagc agtatcttca atcccaatta tagtattctc ccctattggt
     9721 ggagctattg cggatatatt caacagaaga aacttaatgg ttatttttga tttttcaagt
     9781 agtcttacag tactaatctt agcagtattc ttattcaaca ataatggttc aatattactt
     9841 gtaggaatta ttatgaccat attatcagtc ataagcacaa tgtatcaacc aactgttcag
     9901 tcaagtacac cccttttagt tgataatgag catttaatga atgcaaatgg tattgtagct
     9961 ggagttgcat ctcttacaaa tattgcagga cctgtattag gaggtgtctt gtatggtgtg
    10021 attggaatta atgcaattat agttatcagt tgtgtaagtt tctttctatc tgcaattatg
    10081 gagatattta tacagattcc ttttatcaaa caagagcaaa acagcaacat tataaaaact
    10141 atatttactg atataaagga tggtattgtt catatatgta aagaaaatag atttatacta
    10201 aaaatacttt ttttagctat agcaataaac ttatttttaa gctctatgtt aatagttggt
    10261 atcccctata tagtaaaaat cactatggga gctagtaact tcttatatgg tctagcagaa
    10321 ggagcaattt cttttagttc actggtagct gctgttagta ttggtatttt ctcaaaacat
    10381 ctaaaaatca ctaatttgca tatttccttt attatatgtg cagttatgtt cataccaatg
    10441 gcggtttcag tatatccatt tatattaaac acaggatttc cagttccatt tttaatcttt
    10501 atagtatcat ctatgctaat attttttaca acgactctta tctcaatata tgttataaca
    10561 gtcacacaat taaaaactcc aaatgaactc ttaggaaaag taatgtcaat tctttttgca
    10621 ggaacagcaa ttgcaatgcc ccttggtcag gttatgtatg gtaaactttt tgatttatta
    10681 tcagatagag tgtacatcat tatactagta gttggattta ttacattact tatagcattt
    10741 gtatcaaaga taacattgag gaacgaaaat ctttagatac aaagaattta gatataaaga
    10801 tttaattttc actaatttct gaatttaaat gcaaaattgt gtatcagtat gttgacgttt
    10861 tagaaattga ttgatataat caatggaaaa taaactttat aaaaattcaa aatattcagc
    10921 tagaggagtg ataaaaatga gttacatgcc aactttagaa caagcatggg aaatattaag
    10981 aaaatacaat aaggaagaat ttcatataag acatgctcaa attgtatctg gagttatgag
    11041 atactatgca aaggagtacg accctgaaag agtagaattt tgggaaatag ttggactttt
    11101 acatgattta gattttgaaa tgtatcctga tgaacattgc gttaaacaaa gagaattaat
    11161 gacagagctt gatttagata aatcaataat cgattctaca ataagtcatg gatatggatt
    11221 aactggttca gatgttaaac cagagcaatt tatggagaaa gtattatttg ctgtggatga
    11281 acttactggt ttaattggtg ctgctgcaat aatgcgtcct tcaaagagcg tttctgattt
    11341 agaattaaaa tctgttaaga agaaatttaa agataaatca tttgctgctg gctgttctcg
    11401 agacgttata agaagtggtg cagaaatgct tggatgggaa cttgataagc ttatccaaac
    11461 aactatagaa gctatgagaa gcttgattcc agagatggca atatagctcc tccatacata
    11521 taccatagaa gttacatctt taaagagcat ttctcttttt gatgcaactt ctttttatat
    11581 tttgtagaat aaaaagttta atattttaaa ctcactatat aataaataat ataatgtata
    11641 atagtcataa actttctaac cttaaaagtt tctaataatc aataaacaat tctaatatac
    11701 aaatgtcatt atatgattta attatatatc ttcaatttat atattaaaat aaatatatat
    11761 atattaaatt cttatacacc aaatttttac attaaacttc tatgcaccac atttatacat
    11821 attaaattct tatatattaa atttctatgc atcactttta tacatactaa attcctatat
    11881 attaaacttt tatgcatcac ttttatacat attaaatcct atatattaaa cttttatgca
    11941 tcacttt
//
LOCUS       DAELLV010000111        11885 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.111, whole genome shotgun sequence.
ACCESSION   DAELLV010000111 DAELLV010000000
VERSION     DAELLV010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11885
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.111"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            48..884
                     /locus_tag="KPD58_002274"
     CDS             48..884
                     /locus_tag="KPD58_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG0266036.1"
                     /translation="MNIKKIFNNNVVVSSLEDGTEIIVTGAGVGFKKKVGDLIDENLI
                     SKKYFVQDDQRDKYNQILNKTSIEYFKISEEIIEKANEVLNTQVNDSIILALTSHIEF
                     AVQREKQGIKLPNLILNETKQLYREEFEFGLWAIDEIEKRIGIKLPEDEAGYIAIHII
                     NGLENSQKDEGINILEFSKQVIQIIEEVHGFKLDVNSLNYTRLITHLKFFVQRILRKE
                     TYNDSDIEDMYKLVNKNYNKPKVCTEEIAALVLDRFEYKISKEEELYLMIHINKITNS
                     GC"
     gene            1237..2814
                     /locus_tag="KPD58_002275"
     CDS             1237..2814
                     /locus_tag="KPD58_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG0266037.1"
                     /translation="MKKNKLGLWNFFQMLGKTFMFPIALLSVSGMMLGLGAGFTDPKM
                     IEMVPFLGNEYVNLVLNFMLTIGLFAFNNLGALFAMAIPLGLLKKEKEFGAFSGLVGF
                     IAMHIGTNFYLTRADLLVPAEQMSTNGQAMIMGIQTYNTSVLGGIVAGLIVYAIYDKV
                     SNLKIPESLGFYSGPRLVPIVSLIVMSIVGLLIPIVWPPFFNAFQSLGRWISSAGPLG
                     YFSYAVAERVTIPFGLNHLVTSVFRFTPIGGTAVIDGETYFGTLNMFMAYVENNQIIP
                     LDLAGKMEQGKLMIQYGLAGAALAIYRTAKPENRKALKGLLISGVLTVIIGGISEPIE
                     FLFLFISPALFAFHTFMNGLANMVLPYLGVLMGFTGDLIAFISFGVLRGTATGWPIAV
                     LVAVLYFAIYYFVFKWAIIKFNIKTPGREDKEIKVSEGNGSNFKNLSTYKGQQMISAL
                     GGQSNILSLDNCVTRLRLKLQDVSLINEDAIKEAGGIAVVKLDEHTIQVIIGTQVYSL
                     RKQMDKAMEGHLEACND"
     gene            2807..3991
                     /locus_tag="KPD58_002276"
     CDS             2807..3991
                     /locus_tag="KPD58_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HBG0266038.1"
                     /translation="MINFDKKVDRLGTYCTQWDYVEDRFGKKDLLPFTISDMDLEFPF
                     EITEVLQERLNHRVLGYSRWKHDDFRNSVTNWYIKRFNCSVDSNWIYYSPSVMYSISK
                     LIEMLSNEGDGILINTPAYNAFYDVIINNNRNIIKSHLVNRNGFYSIDFNDFEKKCKK
                     SKIFILCNPHNPTGRVWSELELVKMIGICKNNDVKIISDDIHMDIVYDNRMTPILNVA
                     KDYLDSVFICTSASKSFNIPALTGSYVIIPNEGIRNKFENITRYRDFVNSPAILAILA
                     TMVCYDDCEYWLDELLKYLKENLKYTIDYIKDNLEPLKLVMPEGCYFAWIDFSELKIS
                     GEKFQKLLMDIGEVAIMSGSVYGEEGEFYLRLNVACSREKLEDGLNRIKKTMDYIEKR
                     IL"
     gene            4009..4608
                     /locus_tag="KPD58_002277"
     CDS             4009..4608
                     /locus_tag="KPD58_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG0266039.1"
                     /translation="MGYLKTMVDVMTTEMNAIRCLIDEAGIEYESIVSEIANCEGKVI
                     FMGVGKSAHIGKKLAATFASTGTPSFFVHATEAVHGDLGMIESKDITILISNSGNSME
                     VVNCIKYIKAIGSKTIAFTSNRNSVLAKECDYALIYPAKDEADHLNLAPTTSSTITLV
                     LGDSIACALSKSSNFGSSDFYKYHPGGSLGEKLKTANNG"
     gene            4632..5102
                     /locus_tag="KPD58_002278"
     CDS             4632..5102
                     /locus_tag="KPD58_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG0266040.1"
                     /translation="MFKMFKKKSIKSPISGKVIELSKIQDGVFSQKIMGEGVAIDSTG
                     DIVYAPINGKIVVVAETKHAFVIELENGMELLIHVGLDTVNLKGEGFEVLVSMGEQVK
                     EGTPMLKINRPLIESKGISLITPVTITNHSEYNMNTCNVGNSVEGGKDTVIEFK"
     gene            5461..6207
                     /locus_tag="KPD58_002279"
     CDS             5461..6207
                     /locus_tag="KPD58_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HBG0266041.1"
                     /translation="MKIENLSIDDIKRGYIFNDDLNCYSCIKCTKTYEVGEIYKIGNR
                     FFEASKAIELHMELEHSDYLEQLLNSDSKYNTLTENQKQLFQLFISDLPDKEIAKQVG
                     VSTSTIRHQKFIFREKAKQAKFYLALYESVFEDKSNKSSTIIPIHEHARMVDERYVVT
                     EEERKHILDTSFESMNPLKLKVFSSKEKKKVVILSKILEQFERGKKYSEKEVNQILES
                     IYDDFATIRRYLIEYGFMSRNKECTKYWLT"
     gene            6268..6942
                     /locus_tag="KPD58_002280"
     CDS             6268..6942
                     /locus_tag="KPD58_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HBG0266042.1"
                     /translation="MNDELKSILYNKGVDIVRFVDISEFPINQTQGFSKAILFCIGLS
                     KKFITDIYNNLPTDSDEFLEKEEKVEELADWISKYIQNKGYRAYSQSEKNNLEHGYFE
                     KAYINPEMQSGISPLPHKTIANISGIGFMGKNNLFVTEEYGCAFSMCTVLTDAPISVE
                     RYPLIDSKCMDCNVCVENCPAKAIHGNEWTLPGKRESIIDVSKCFCVLRCMMSCPWSL
                     RYANQK"
     gene            7311..8657
                     /locus_tag="KPD58_002281"
     CDS             7311..8657
                     /locus_tag="KPD58_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBG0266043.1"
                     /translation="MEIETKTPDWINVLELNSKIDITGKYDVSNMIQNIVSDKSLEGS
                     IIYFPKGNYLFEKGIKISQQITLQGDSYYGRGNQVSNLDKKQSIIGTTNFITRGVSNM
                     SIITLSGTSQCIKNINFYYDSHDIEKIPPKNVSAITEYGETQGLSHFEHLFISGFSGI
                     GIEIPYYSTGNDITVSSCGLGMRLGEKSMLSSSKIYECKNGMEITTGVSLNNVRIEEI
                     KEIGINNKGFGFNLIMNLMVDQCGYCGFMFEKMCYSQITARITRCGQYYKSVDYNAYE
                     NMEDRKEEAYSIFYGDILENCNIVLMNRNIDTLESDSLNEHKIYVIKANETNNVMLTC
                     YAEADNFIQCAKGNLLLENGENTYKFYNGEICSVGGVQISDIDEKDLIKIIENTLYID
                     DKGKYLMVPRLKKNTVIYSVLDSTEEINKLHGGIWEQIDVKVLAGESMYYYKKIKD"
     gene            8684..10084
                     /locus_tag="KPD58_002282"
     CDS             8684..10084
                     /locus_tag="KPD58_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266044.1"
                     /translation="MEESIIIKDISGWINVLDYGICNDETKDVSFKLQEIMDAVPEDS
                     IIYFPKGKYLFESGVKIKKRLTLCGDSYILANVPKNTAGVTQFTFKGKKNPKSENRAI
                     ITAKSVRHCIKNICFYSDSCDRDMTSNHEPPTDGKPRFHHNVIINYRNISAVICEDKQ
                     GEPGHYENLSFSGFSGIALDMPNNSTANDITIFTSGLGISTGENSTIANSKVWGCVDG
                     MIISTGTFINGMRVEEIKKVGIKNIGRGLNFITNITIDQCGYCGVWFDSISNVQISGN
                     ITRCGQYYYDVSYDNYLMLDNRVKEAYSLFYGNLLESSNIVLSNTNKDNWEDFKEDKH
                     KVYLVEALETKNVLLTCNIDATDWIVKSEKGNLTYNNMRQTVIFYDGKLSSINGIGIS
                     DEDYGDKVKIKKGNIYINSKDKDIKMHKPEVGDIMSTIIKDKSILSAHYGGTWEKIGE
                     KIEFGITVHYYKLIEK"
     gene            10294..11730
                     /locus_tag="KPD58_002283"
     CDS             10294..11730
                     /locus_tag="KPD58_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBG0266045.1"
                     /translation="MEGRTGIKDGRWINVVERGIKNDGSYNIGPALQDIIDEIETDFC
                     VLYFPKGKYLFTSGVKINKEIVLQGDSNSSTGTTQFITRGVENMSIITLTGKKQCIKN
                     INFYSDSCEIQVNKEPPTKGNPKYHYEMIINKNEKNVEMNNVSAILYDNSKNMIKGLG
                     HYENIYISGFSGTGIRIPYYSIVNDITVSSCGLGIDTGIDTIISNSRISKCQNGMRIR
                     TGTSINNVRIEKIQKVGLISSQVKDYEGYGSYKINNITIDQCGYCGFLFDSVKGSYFS
                     GIIRRCGQYYYNTDYDTYLSIKDRVEEAYSLFYGNYFTECNIDLVSDNKDNWDDGLEY
                     KHKVYVFKTLQIPKLTLRCNIDAYDYIVKSVHGGGNLMLKNNFNNYIFIDSSNPEVIN
                     GINFNYQKNGNLLRLNNGKLSYKNGKEETAIFRPNYGLIVASTDSECEQIETSYGGKW
                     EKLGSEVIGKETVYYYKHHEPDKTNQIQ"
BASE COUNT         4613 a         1165 c         2203 g         3904 t
ORIGIN      
        1 tatactatgt aagtgtgatg agagaatttt tttagagggg atttatagtg aatataaaaa
       61 agatatttaa taacaatgta gtagtaagct ccctagaaga tggcacagaa ataattgtca
      121 ctggtgctgg agtaggattt aagaaaaaag taggcgattt gatagatgaa aatttgattt
      181 ctaagaaata ttttgttcaa gacgatcaaa gggacaaata caatcaaata cttaataaga
      241 cctctattga atactttaaa atttcagaag aaattataga aaaggctaat gaagttttaa
      301 atactcaagt gaatgattca ataatattag ccttaactag tcacatagaa tttgctgtcc
      361 aaagagaaaa acaaggtata aaattaccaa acttaatatt gaatgagaca aaacaacttt
      421 atagggaaga atttgaattt ggattatggg caattgatga aattgaaaaa aggataggaa
      481 taaaattgcc tgaagatgaa gcaggatata ttgctattca cataatcaat ggtttggaaa
      541 attctcaaaa agatgaaggt attaatatac tagagttttc aaaacaagtt attcagataa
      601 ttgaagaagt acatggattt aaattggatg taaattcatt aaattatact agacttataa
      661 ctcacttaaa gttttttgtt caaaggattc tccgaaaaga aacttacaat gatagtgata
      721 ttgaagatat gtataagtta gtgaataaaa attacaataa acctaaagtg tgtactgaag
      781 aaattgcagc attggtatta gacagatttg aatataagat tagtaaagaa gaagaattat
      841 atttaatgat tcacataaat aaaataacaa acagtggttg ttaggattgt aactgataaa
      901 gcaggcaaaa cctaagtcat tgagcttaac tattagatta agttttttga tttaggtttt
      961 tttattagat acaatcaaaa tacagacagt gacttttagg attgtaactg gttaagcagg
     1021 caaaacctaa atcattgaat ttaactatta ggttaagttt tttgatttag gtttttttat
     1081 tagatacaat caaatacaga cagtggttgt taggattgta actggttgag caggcaaaac
     1141 ctaaatcatt aaacttaatt attaaattaa gttttttgat ttaggttttt ctataaattt
     1201 gattatgttt ttagataaaa ataggagggg aaaattatga aaaaaaataa gttaggttta
     1261 tggaacttct tccaaatgtt aggaaaaacg tttatgttcc caatagcact acttagtgtt
     1321 tctgggatga tgctaggact tggggcaggt tttacagacc ctaagatgat tgaaatggtt
     1381 ccatttttag ggaatgaata tgttaactta gttttaaact tcatgttaac aataggatta
     1441 tttgcattta acaacttggg agcattattt gcaatggcaa ttccactagg attattaaaa
     1501 aaagaaaaag agtttggtgc tttttcagga ttagtaggat ttatagcaat gcatataggt
     1561 acaaattttt atttaacaag agctgatttg ttagtaccag cagaacagat gtcaactaat
     1621 ggtcaagcta tgattatggg tatacaaaca tataatacaa gtgttttagg tggtattgta
     1681 gcaggattaa ttgtttatgc aatatatgat aaagttagta atttaaagat accagaatca
     1741 ttaggattct acagtggacc aagattagtt ccaattgtta gtttaatagt tatgagtata
     1801 gttggattat taatacctat tgtttggcca ccatttttca atgcattcca aagtttagga
     1861 agatggattt cttcagcagg accattagga tatttttcat atgcagtggc agaaagagtt
     1921 acaattccat ttggactaaa tcatcttgta acatctgtat tccgttttac accaataggt
     1981 ggaactgctg ttattgatgg agaaacgtac tttggtacat tgaacatgtt tatggcatat
     2041 gtagaaaata atcagattat acctcttgat ttagctggta aaatggaaca aggtaaattg
     2101 atgattcaat atggattagc tggtgctgca ttggctatct accgtacagc aaaaccagaa
     2161 aatagaaagg cacttaaagg acttttaata tcaggagtgt tgacagttat tataggtggt
     2221 ataagtgaac caatagagtt tctattctta ttcatatcac cagcattatt tgctttccat
     2281 acatttatga atggtctagc aaacatggta cttccatact taggcgtgtt gatgggattc
     2341 acaggagatt taattgcatt tataagtttt ggtgtgttaa gaggaacagc tacaggatgg
     2401 ccaatagcag tattagtagc agttttatat tttgcaatat actattttgt atttaagtgg
     2461 gcaattatta agtttaatat aaagacccca ggacgtgagg ataaagaaat aaaagtatct
     2521 gaaggaaatg gttcaaattt caaaaatttg tctacatata aaggacaaca aatgatatct
     2581 gctttaggtg gtcaatctaa tatattatca ctagataatt gtgtaactag attacgttta
     2641 aaattacaag atgtatcatt aataaatgag gatgcaatta aagaggctgg tggtatagca
     2701 gttgttaaat tggatgagca tacaatacaa gtaattatag gaacccaagt gtattcttta
     2761 agaaagcaga tggataaagc tatggaaggt catttggagg catgtaatga ttaattttga
     2821 taaaaaagta gaccgattgg gtacatattg tacccagtgg gactatgtag aagatagatt
     2881 tggtaagaag gatttgcttc cttttacaat ttcagatatg gatttagagt ttccatttga
     2941 aataacagag gtgttgcagg aaaggttaaa tcatagagtt ttgggataca gtagatggaa
     3001 acatgatgat tttagaaatt cagttacgaa ttggtatatt aaaagattta attgtagcgt
     3061 tgatagtaac tggatatatt atagtccaag tgtaatgtat tcaattagca aacttattga
     3121 gatgttaagt aatgaaggag atggaatcct tataaatact cctgcataca atgcttttta
     3181 tgatgtgata ataaataaca atcgtaatat tataaagtca catcttgtaa ataggaatgg
     3241 attctattca attgatttta atgattttga gaaaaagtgt aaaaaatcta agatatttat
     3301 tttatgtaat cctcacaatc caactggtag agtatggagt gagttagaac ttgttaagat
     3361 gataggaatt tgtaagaata atgatgtcaa gataatatca gatgatatac acatggatat
     3421 tgtatatgac aatagaatga cacctatctt aaatgttgca aaggactatt tagattctgt
     3481 attcatttgt acatcagcgt ctaaatcttt caatatacca gcattgactg ggtcatatgt
     3541 gattatacca aatgaaggta ttagaaataa atttgaaaat atcactagat accgtgattt
     3601 tgtaaattcg ccagcaattc tagcaatatt agccacaatg gtctgttatg atgactgcga
     3661 atattggtta gatgaactat taaaatacct taaagagaat cttaaatata cgattgatta
     3721 tataaaagat aatttagaac cattgaaatt ggtcatgcca gaaggctgtt actttgcttg
     3781 gatagatttt tccgaactta aaataagtgg tgagaaattc caaaaattat tgatggatat
     3841 tggagaagtt gctattatgt cggggtctgt atatggtgaa gaaggtgagt tttatttaag
     3901 gttaaatgtg gcatgttcaa gagaaaaact tgaagatggt ttaaatagga ttaaaaaaac
     3961 tatggattat attgaaaaaa gaattttata gtaggaaggg agatagttat gggatattta
     4021 aaaacaatgg ttgatgttat gactactgaa atgaatgcca taagatgttt aatagatgaa
     4081 gcaggaatag aatatgaaag tatagttagt gaaattgcaa attgtgaagg caaagtaatt
     4141 tttatgggtg taggaaaatc tgcacatata ggtaagaaat tggcagctac atttgctagt
     4201 actggtacac ctagcttttt tgtacatgca acagaggcag tgcatggaga tttaggaatg
     4261 attgaaagta aggatattac aattttaata tcaaatagtg gaaattctat ggaagtggta
     4321 aactgtataa aatatattaa agcaataggt tctaagacaa ttgcatttac ttcaaacaga
     4381 aattcagtac tggcaaaaga gtgtgattat gctttaatat atcctgcaaa ggatgaagca
     4441 gaccatttaa atctagcacc aacaacatca tctacaatta ctttggtttt aggagattct
     4501 attgcatgtg cattatctaa gagtagtaat tttggttctt cagattttta taagtaccat
     4561 ccaggaggaa gtttaggtga gaagttaaaa acagctaaca atggataaaa gcaaaaagga
     4621 gagtagtttt tatgttcaaa atgtttaaaa aaaaatcaat aaaatcaccc atatcaggaa
     4681 aagtaataga gttatctaaa atacaagatg gtgtattttc tcaaaaaatt atgggagagg
     4741 gtgtagctat agattctaca ggagacattg tatatgctcc aatcaatgga aagatagtag
     4801 tagtagcaga gactaaacat gcatttgtaa tagaattaga aaatgggatg gaacttctta
     4861 ttcatgttgg tttagatact gtaaatttaa aaggcgaagg ttttgaggta ttggtgagta
     4921 tgggagaaca agtgaaggaa ggaacaccaa tgttgaaaat taaccgtcct ttgattgaat
     4981 caaagggtat atcacttatt actccagtta caataactaa tcattcagag tacaatatga
     5041 atacatgtaa tgtaggcaat agtgttgaag gtgggaaaga tactgtaatt gagtttaaat
     5101 aaagaactaa aatacaaggt taatatttat aaatacctat aaacttatgt tttataagat
     5161 tgattgtata gatttgttgt ataaaaaaga actctttaat tataaattaa ggagttcttt
     5221 tttattttgt aaatattgtt tagatttata tagaaaaaca cttagtaata aatagaataa
     5281 ataagtattt atatagctaa aaatctaaat agttttgttt aaatatattt ttatattttg
     5341 taatttcaaa ttaataaata attagatttg ataaacaaaa aataaaaatg ttgattacat
     5401 taataaactt taatataata tatttatgaa aaatgtaaac tgtgaaatgg aggtagtaac
     5461 atgaaaattg aaaatctttc tattgatgat attaaacgag gctatatatt taatgatgat
     5521 ttaaattgtt atagttgcat taaatgtaca aaaacatatg aggtaggaga aatatataaa
     5581 ataggtaata gattctttga agcttcaaaa gctattgaac ttcatatgga attagaacat
     5641 agtgattatt tagagcaatt gcttaatagt gattcaaagt acaacacatt gactgaaaat
     5701 caaaaacaat tatttcaatt atttattagt gatttaccag ataaggagat agcaaaacag
     5761 gttggagtat ccacatctac tatacgccat caaaaattta ttttcagaga aaaagcaaaa
     5821 caggctaagt tctatttagc actatatgaa tctgtatttg aagataaatc taataaaagt
     5881 agtactataa taccaatcca tgaacatgct cgaatggtag atgaaagata tgtagtaact
     5941 gaagaagaga gaaaacacat tttggataca tcatttgaaa gtatgaatcc tttaaaatta
     6001 aaagtatttt cttcaaaaga aaagaaaaag gtcgtaattt tatctaagat tttggaacaa
     6061 tttgaacgtg ggaagaaata ttcagaaaag gaagttaatc agattttaga atctatatat
     6121 gatgattttg caacaattag aagatatcta attgagtatg gttttatgag tagaaataaa
     6181 gaatgtacaa aatattggtt aacataatta aaataataaa ttttactaat gtgtaatgtt
     6241 atttttatct agggggagga gtcaaatatg aatgatgaat taaaatctat tttatacaat
     6301 aaaggtgtag acattgttcg ctttgttgat atatctgaat ttccaataaa tcaaacacaa
     6361 ggtttttcta aagcaatttt attttgtata gggttatcaa aaaaatttat tacagatata
     6421 tacaataact tacctacaga tagtgatgag tttttggaaa aagaggagaa agtagaagaa
     6481 ttagctgact ggatttcaaa atatattcaa aataaaggat atagagcgta ttctcaatcg
     6541 gagaaaaaca atctagaaca tggatatttt gaaaaagcct atattaatcc agaaatgcaa
     6601 tcagggatta gtcctttacc acataagaca atagcaaata ttagtggcat tggatttatg
     6661 ggaaaaaata atttgtttgt aacagaggaa tatggatgtg cttttagcat gtgtacagta
     6721 cttacagatg ctccaatttc tgttgaaaga tatcctttga ttgattcaaa atgtatggat
     6781 tgcaatgttt gtgtagagaa ctgtcctgca aaagctatac atggaaatga atggacttta
     6841 cctggaaaaa gagaatctat tattgatgtt tcaaagtgtt tttgtgtatt gaggtgtatg
     6901 atgagttgcc catggtcatt aagatatgca aatcaaaaat aactttacta gagaaggtat
     6961 taaattgaat attataaaat ttttaaaatg taagattata taagattttt caaaataaaa
     7021 atttgtatag aacactcaga aatgagtgtt tttctattgg gattaaatta tattgataca
     7081 aagcttaact attttaatca ttatataaaa tttgtattaa aaaattaaga ataacttggt
     7141 aatagtgaaa atataatgag attataattg atttataata taactataat gatattgtaa
     7201 aaatgaaaat tgtcctctca aaattcccaa agagagtaaa agtcacttca gttatagtca
     7261 aaaaaatttt tctgtgaaaa gaatttagat ttgaaaagga ggaattaatc atggaaattg
     7321 aaactaaaac tccagattgg attaatgtac tagaattaaa cagtaaaata gatattactg
     7381 ggaaatatga tgtaagtaat atgatacaaa atattgtctc tgataagtca ctagaaggtt
     7441 ccattatata ttttccaaaa ggtaattact tatttgagaa aggtattaaa attagtcaac
     7501 aaataacact tcaaggagat tcttattatg gaagaggtaa tcaagtatca aatttagata
     7561 aaaaacaatc aatcatagga acaaccaatt ttataacccg aggagtttct aatatgagta
     7621 ttattacatt gtcaggaaca agtcaatgta tcaaaaatat taatttttat tatgatagtc
     7681 atgatataga gaaaataccg cctaaaaatg tatctgctat tacagaatat ggagaaacac
     7741 aaggactatc tcattttgaa catctgttta tttctggatt ttcagggata ggaattgaaa
     7801 ttccatacta ttcaactggt aatgatatta ctgttagttc atgtggtctt ggtatgaggc
     7861 ttggagagaa gtcaatgctt tctagtagca agatttacga atgtaaaaat ggaatggaga
     7921 ttactactgg tgttagctta aataatgtga gaatagaaga aataaaagaa ataggtatta
     7981 ataataaagg atttggtttt aacctgatta tgaatcttat ggttgatcaa tgtggatatt
     8041 gtggatttat gtttgaaaaa atgtgctata gccagataac tgccagaatt actagatgcg
     8101 gtcagtatta taaaagtgtt gattataatg cttatgagaa tatggaagat aggaaagaag
     8161 aagcttattc cattttttat ggagatattt tagaaaattg caatattgta ttgatgaata
     8221 gaaatattga taccttagaa agtgattcac ttaatgaaca taagatatat gtaatcaagg
     8281 caaatgaaac aaacaatgta atgctaacat gttatgctga agcagataac tttatacaat
     8341 gtgcaaaggg caacttgctg ttagaaaatg gtgaaaatac ttataaattt tataatggtg
     8401 agatttgttc tgtaggaggt gttcaaattt ctgatataga tgaaaaagat ttaatcaaaa
     8461 taatagagaa cactttgtat atagatgata aaggtaagta tttaatggtt cctagattaa
     8521 aaaagaatac tgtaatatat tcagtactag attctacaga agaaataaat aaattacatg
     8581 gtggaatatg ggaacagatt gatgtaaaag tattagctgg ggaatccatg tactattata
     8641 aaaagataaa agattaaatt atcaaactag gaggtagtga ttaatggaag agtctataat
     8701 aattaaagat ataagtggct ggattaatgt acttgattat ggaatttgta atgatgaaac
     8761 aaaagatgta agttttaagt tgcaagagat tatggatgct gtaccagaag attctattat
     8821 atattttcca aaaggaaaat acttatttga aagtggtgtt aagattaaga aacgattaac
     8881 cttatgtgga gattcttata ttttagcgaa tgttcctaaa aatactgctg gagtaacaca
     8941 gtttactttt aaaggaaaga aaaatcctaa aagtgaaaat agagcaatca taacagcaaa
     9001 aagtgtaaga cactgtatta agaacatttg cttttattca gatagttgtg atagagatat
     9061 gacatctaat catgaacctc caacagatgg aaaacctaga tttcatcata atgtaattat
     9121 aaattataga aatatatctg ctgttatatg tgaagataaa cagggagaac caggtcatta
     9181 tgaaaatcta tcttttagtg gtttttctgg aattgcactt gatatgccta ataattctac
     9241 agcaaatgat attacaattt ttacatccgg acttggaatt agtacagggg aaaattcaac
     9301 aattgctaac agtaaggttt ggggctgtgt tgatggaatg ataatttcta ctggaacttt
     9361 tataaatggt atgagggtag aagaaataaa aaaagttgga attaaaaata ttggtagagg
     9421 tttaaatttt attacaaata tcacaattga ccaatgtgga tattgtggag tttggtttga
     9481 ttctatatcc aatgttcaga tatcaggaaa tatcacaagg tgtgggcagt actattatga
     9541 tgtaagctat gataattatt taatgttgga taatagagtt aaagaagcat attctttatt
     9601 ctatggtaat ttattggaat caagtaatat agttttatca aataccaata aagataattg
     9661 ggaagatttc aaagaagata agcataaggt gtatcttgta gaagcacttg aaaccaagaa
     9721 tgtactatta acatgcaata tagatgctac tgactggatt gttaagtcag aaaaaggaaa
     9781 tttaacgtac aataatatgc gacaaactgt tatattttac gatggaaaac taagtagtat
     9841 caatggaatt ggtatatctg atgaagatta tggggataaa gtcaagataa aaaaaggaaa
     9901 tatatatata aatagtaagg ataaggatat taagatgcat aaaccagagg taggtgacat
     9961 tatgtcaaca attattaaag ataaaagtat tctttctgct cattatgggg gaacatggga
    10021 gaaaattggt gaaaaaatag agtttggaat aactgtacac tattataaat tgatagagaa
    10081 ataagtaaat acgtagtatt tgaatagatg tattttattg gagagctagg actatagttt
    10141 taatgtaaat ggtttggtaa tagcatggat tgaataatat aaaaataata taaaaaatta
    10201 atttgattat gtaaaattct atgaagtagt aaaactgtga ctttagtcat aggaggttca
    10261 aactaagtaa aagggaaagg aatatataat attatggaag gaagaactgg gattaaagat
    10321 ggaagatgga ttaatgtagt agaaagaggt attaaaaatg atggttcgta caacataggt
    10381 cctgctttgc aggatattat tgatgaaata gaaacagatt tttgtgtctt atattttcca
    10441 aaaggtaaat acctatttac aagtggtgtt aaaattaata aagaaattgt attgcaaggt
    10501 gattctaatt catctacagg tactacacaa tttataactc gtggagtaga aaatatgagt
    10561 attattacat tgacagggaa gaaacaatgt attaaaaata taaatttcta ttctgatagt
    10621 tgtgagatac aggtaaataa agaaccaccg acaaaaggta atcctaaata tcattatgag
    10681 atgattatca ataagaatga aaagaatgtt gagatgaaca atgtatcagc aatcctgtat
    10741 gataacagta agaatatgat taaaggtcta ggccattatg aaaatattta tataagtggt
    10801 ttttcgggta caggaattag gataccatat tactcaattg taaatgatat tacagtttca
    10861 tcttgtgggc tgggtattga tacaggaata gatacaatta tttctaatag tagaatatct
    10921 aagtgtcaaa atggaatgag gattaggact ggaactagca taaataatgt aagaattgaa
    10981 aaaatacaga aagttggtct tataagtagt caagttaagg attatgaggg atatggaagt
    11041 tataaaatta ataatattac gattgaccaa tgtgggtatt gtggtttttt atttgactca
    11101 gtgaaaggta gttatttttc tggtattata agaagatgtg ggcagtacta ttataataca
    11161 gattatgaca catatttaag tattaaagat agagtagaag aagcatattc tttattttat
    11221 ggaaattatt ttacagaatg taatattgat ttagttagtg ataacaagga taactgggat
    11281 gatggcttag aatataaaca taaagtttat gtatttaaaa cccttcaaat accaaaatta
    11341 acattgagat gcaatataga tgcctatgat tatatcgtta aatcggtaca tggaggaggt
    11401 aatttaatgt taaaaaataa ctttaacaat tatatattta ttgattcatc taatccagaa
    11461 gttattaatg gaattaattt taattatcaa aagaatggaa acttgttaag gttaaataat
    11521 ggtaaattaa gttataaaaa tggaaaggaa gaaacagcta tattcagacc taattatggt
    11581 cttattgtag catcaacaga ttcagaatgt gaacaaatag agactagcta tggaggaaaa
    11641 tgggagaagc ttggttctga agtaattgga aaagaaacgg tatattatta caaacatcat
    11701 gaaccagata aaacaaacca aatacaataa aatttgtatt atataatgat ataaagtttt
    11761 aatatatagt gtttatcttt aatgtaatat tggtatattt gtatagtatt aaatttgtta
    11821 aaaagtaaaa ttgtagggtg cttgaaaata gcaccttttt tagtttcatt ataaaattag
    11881 ttttg
//
LOCUS       DAELLV010000112        11748 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.112, whole genome shotgun sequence.
ACCESSION   DAELLV010000112 DAELLV010000000
VERSION     DAELLV010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11748
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.112"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..3074
                     /locus_tag="KPD58_002284"
     CDS             <1..3074
                     /locus_tag="KPD58_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBG0266046.1"
                     /translation="LDEIVYVSSLDTYELLYINEIGKKAMGLNDISHLKCYKALQGKD
                     SPCEFCTNSILKEDEYYIWENTNTRTNRHFILKDKLIKWEGKHARLEIALDITEKEDI
                     SKSIAEKLEIKELLVGCIKHLITESNFSDAVNLVLTNIGKFHKADRAYVFEVSEDRKK
                     VTNTYEWCNEGVDEQKQALTNLDINELKNWFLLFEQKGFVIIDDIENIKEEQPIEYEI
                     LKPQKIESLMTSSLKQDDKVVGFIGVDNPHKAVGDTSLLTSLSYFLLTEMKKRKVENK
                     LNYMSYYDFLTGAYNRNKYIKYIDNFQKGKVKSIGVVFVDINGLKDINDNFGHANGDS
                     AIVSACVIIKKYFHSYNVYRIGGDEFVVLCEDISKQLFEELVSWLSEEFKDEMIYSVA
                     VGSIWSDDNIDIHKLIKVADERMYQDKKRYYNKNKLRNNEMKVISDLADSTNNILFKH
                     KELVDKVYFDDLMIKGYSKAYEKLAYNTYDIILSICIDTNLITPIYQSESNIFSFPIK
                     ENLEKTFSYIKKHLIHPDDIILFNDNHLLKSLASLNNSKQDDLELNFEYRRLGIDKKY
                     YWALIYMLKISSKNKLGEKQTHILVAIKNIDYLMQLHKNQKDTLTGLYNYESFCEQIS
                     LMLKRYPDKRYAIIMMDIDKFKVINDIYGVRGGDKALIYVAELIQKNIGSNGICCRMY
                     ADVFCIFYESYSDRDIHKVIYKIKNSLNRKKFEYMLVPCFGVYRILDNHIPITNACEM
                     AGYAHKKVKKQSINNYIFYDDTIRNDALEEKQMEREMEYALENGQFHVYLQPKYNLNA
                     NKIVGAEALVRWVHPEKGIIPPIKFISLFEKNGFIIRLDMYVWEQVCSLIRKWIDLGE
                     EPVPISVNVSRLHLHNPHFKEIVLSLINKYSIPKSFLELELTESLFIKNIKLFTKIIA
                     DLRMDGIVLNMDDFGSAYSSLNMLKNINIDTLKIDCGFFNEEGITDREKIVLKHTIAM
                     AKDLDMDVVAEGVETKEQAEFLVSLDCVVIQGYYYCKPIPSADFEKLLYQK"
     gene            3197..4483
                     /locus_tag="KPD58_002285"
     CDS             3197..4483
                     /locus_tag="KPD58_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG0266047.1"
                     /translation="MIVGIIGPSDSGFKIKDDLKQIDSDLKTKIYVREKVVDTIEVIS
                     KCEDECDAIIFTGCAVYEFIKSKYEISKPHSFVPRSGTSIMKAFWAIKSANIKLDKFS
                     IDVVDRYVVEDALKEFDIKATSVFCNPFSLEVGESELVEWHIKLFEENKTDIMLTGFG
                     AVYNELKERGYPVFRLQATIQLIKESYDKVKSEYALSKARFSQIAVEILSLIDYKEKI
                     DNYYSDMIKKSDMDKLVVNYVRSIQGSLFLLGRNDYVVFVHKGVADNEYNYDKLFNLK
                     REIKDMGFSLSIGIGTGVTAYQAENNAHKALRHSIDSKEIGIYLVDEDENIRGPLASE
                     NELNYSLMLSDEKVIEISKKTGMSCESVAKIIAINENRKSKIYDSKELAEYLNVSERS
                     ARRILNKIVNAGLGRICAKETSIGGGRPKNITEILF"
     gene            complement(4862..5968)
                     /locus_tag="KPD58_002286"
     CDS             complement(4862..5968)
                     /locus_tag="KPD58_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBG0266048.1"
                     /translation="MLDKNRLINNFMDMVRIDSPSNQELEMSKWLVNYLKERNIDAII
                     DDAGEKYGGNTGNVIAYIKGEEGSRPLCLCAHMDQVQPCLGVKPILDGNVVRSDGTTT
                     LGADDKAGIAAILEALEHVITEKIPHRDIYLCFTICEEAGMHGVKNFNPDNLPCKDMV
                     ILDSGGAIGSIAYKAPAQQSIKISFQGKKAHAGIEPEKGLNAILVASHAISNMHIGRI
                     DSLTTSNIGEIEGGGATNIVTDKVTLTAEIRSHIPETLEYELNHMEKCCKDAASKFNT
                     TYTFEHNMSYPSFELSRDSHVFKLSEEAIRQVGVVPNPMVIGGGSDANILANLGYNCA
                     ILSLGMYDVHTVNEYVNIDELYDTAKIVYHMIKL"
     gene            complement(6003..7544)
                     /locus_tag="KPD58_002287"
     CDS             complement(6003..7544)
                     /locus_tag="KPD58_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBG0266049.1"
                     /translation="MNDNVVKKKNFFDRTLNRIETVGNKLPDPVTIFLGLCVLLLILS
                     SLVGSMGISVVHPGTGETITAVNLLTVEQLQILLGNIVSNFQGFAPLGLVLVTMIGAG
                     VCDKTGLMTATIKASVSKIPETRVTLVVMTIGMLANIASDAGTILFPPLAALVYLGVG
                     RHPLIGLFSGYAAVCLGFAANIMISVNDILAASFTVPAAQMIDSGFQANATMNLFFMI
                     ASTFVLILLATWVTEKIVAPRFGKYEGNAELDVDGSLSDVEKKGLKKAGISILIYVAI
                     IVALSVIGKKPFLADPETGDLLSNNAPLMKGMVPIIALAFFIPGVVYGKTIGKIKKDK
                     DVVSIMASAMSDMGGYIILAFAASQFLQLFTNSNLGLILAVKGGEFLKSAGINGPLLL
                     IGFIILSCFINLFIGSASAKWAILAPIFVPMFMMVGYNPALTQMAYRIGDCITNPLSP
                     LFPYFPIILAFTRKYDKDAGMGTVIANMIPYSISFLIVWTILLILFMVFNIPLGPGIL
                     PSYSM"
     gene            complement(7906..8652)
                     /locus_tag="KPD58_002288"
     CDS             complement(7906..8652)
                     /locus_tag="KPD58_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0266050.1"
                     /translation="MFNAGNRIINTWLYPIQDGFVLIDTGYENGFKHFKKRISKFNIK
                     IKDIRYIFLTHAHDDHVGFLNQILDESPNTKIVMHDKALEILYKGQNSFIGGCTSRTA
                     FLFCQIMKLFGKGEHSFSPLKQEFEKRCILVSDKNRKEIEKELNGKIIDTPGHTDDSI
                     SLLIDDGVLFCGDSAMNGFPSTHKITIFAESKLEFIQSWKTIINTKPKMIYPGHGKPF
                     EYSKLEQNLIYVNKINLYPLHNSKDIHRFL"
     gene            complement(8864..10174)
                     /locus_tag="KPD58_002289"
     CDS             complement(8864..10174)
                     /locus_tag="KPD58_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0266051.1"
                     /translation="MNKEQLKSLVIKTIDENREKILKIGQGIYKNPEYGYKEFKTTEA
                     VSNFFEQELNLSVEKNIAVTGCKADANSSKKGPHISILGELDGISCKEHKDANEIGAS
                     HTCGHNIQIAGMLGAAVGLVKSGVLEHLDGKVSFMATPAEEFIELEYREQLKKEGKIT
                     YFGGKQELIKRGYFDDIDISMMFHASDLGEDKALVGPESNGFVGKKVKFIGKESHAGS
                     APHDGINALNAAMLAINNVNALRETFKESERVRFHPIITKGGDIVNVVPADVHMESYV
                     RARTINGMIDANERINKALMAGGMAVGADVEITEIPGYLPILRYKSLDNLFKNNLEDL
                     GLKGKVIDGGDFTGSFDFGDVSHIMPTLHPMIGGVKGALHTRDFEIVDEDLAYIIPAK
                     AMALTVVDLLFDNAKDAKDILDTFTPVMTKKEYLTFLEKHDKTL"
     gene            complement(10187..10657)
                     /locus_tag="KPD58_002290"
     CDS             complement(10187..10657)
                     /locus_tag="KPD58_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266052.1"
                     /translation="MFANLNLKKSSYQLLVMSVIGVIILIGQRISVGTSIVTALPGMV
                     MLILAAMAAMIIKDLFPKSIFPAFGFATIIGLLLSMPYSPTSEVFLTNTNNINFMAIT
                     TPLLAFAGISVGNKIEALKEMSWKIVIISLIVFTTIFFACASIAHIVLSIQGKI"
     gene            complement(10661..11512)
                     /locus_tag="KPD58_002291"
     CDS             complement(10661..11512)
                     /locus_tag="KPD58_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HBG0266053.1"
                     /translation="MEINKKHFIQVFLTCLILVVICEFIGQRTIAIGKASIVLFPMLY
                     AVIIGLIITPDLLGKKIKALKKVVGEKEINIAGEVVGIALVMLGIKYGTTVGPNIDKI
                     IQAGPAFIAQEFGHILAPIVALPLALMFGMKREAVGATASISREPSLGVISEKYGINS
                     PEGSGVLGTYLMGTVLGTIYFSVLGSISIYSGLHPYALGMACGVGSGSMMTAAAGSLS
                     TMVPPEMADTILAYAATSNMMSSITGIIFLVFVSLPFTNFMYKILEPKFSRNKKTKEI
                     KSTEGEC"
BASE COUNT         4297 a         1649 c         1775 g         4027 t
ORIGIN      
        1 agttggatga aattgtatat gtttcttctt tagatacata tgaactttta tatattaatg
       61 aaattggtaa aaaggcaatg ggacttaatg atatatccca tttaaaatgt tataaagcat
      121 tacaggggaa agattctccc tgcgaatttt gtacaaactc tattttaaaa gaagatgaat
      181 attacatatg ggaaaatacg aatactcgaa caaatagaca ttttatatta aaggacaagt
      241 taattaaatg ggagggcaaa catgcacgat tagaaatagc tcttgatatt acagaaaaag
      301 aagatataag taagtcaata gcagaaaaat tagagataaa ggagttatta gttggatgta
      361 ttaaacatct tataacagaa tctaattttt ctgatgctgt aaatcttgta ttaaccaata
      421 ttggtaaatt tcataaagct gatagagcat atgtttttga agtatcagaa gatagaaaaa
      481 aagtaactaa tacatatgaa tggtgcaatg aaggtgttga tgaacaaaaa caggcactaa
      541 caaatcttga tattaatgaa ttaaagaatt ggtttttatt attcgaacaa aaaggttttg
      601 taataattga tgatatagaa aatataaaag aagaacagcc aatagaatat gaaattttaa
      661 aacctcaaaa aattgaaagt ttaatgacat cttcactaaa acaagatgat aaagtagtag
      721 ggtttattgg agttgataat ccacataaag ctgttggaga tacctctctt ttgacatccc
      781 tatcttattt tttattaact gagatgaaaa aaagaaaagt agaaaataaa ttaaattata
      841 tgagctacta tgacttcttg actggtgcat ataatagaaa taaatatatt aagtatatag
      901 ataattttca aaaaggaaaa gttaaatcca taggagttgt atttgtagat ataaatgggt
      961 taaaagatat taatgataac tttggacatg ctaatggcga ctctgctatt gtgtcagcat
     1021 gtgtaataat aaaaaaatac tttcattctt ataatgttta ccgtattgga ggagatgagt
     1081 ttgttgtatt atgtgaagat atatcaaaac aactatttga agaattagtg agttggttaa
     1141 gtgaagaatt taaggatgag atgatatata gtgtagcagt gggcagtatt tggagtgacg
     1201 ataatattga tatacataag ctaattaaag ttgctgatga gagaatgtac caagataaga
     1261 agaggtatta taataagaac aaacttagaa ataatgaaat gaaagtaatt agtgacttag
     1321 ctgattcgac gaataatatt ttatttaaac ataaagagtt agttgataag gtttattttg
     1381 atgatttaat gataaaaggg tattctaagg cttatgaaaa attagcttat aatacatatg
     1441 atataatttt atctatttgc attgatacga atttaattac tccaatttat caatcagaga
     1501 gcaatatttt tagttttcct ataaaggaaa atcttgaaaa aactttttca tacataaaaa
     1561 aacatttaat tcatcctgat gatataatat tatttaatga taaccacctt ctaaaatctc
     1621 ttgctagtct taataatagc aagcaagacg atttagaact aaattttgag tatagacgtt
     1681 taggaataga taaaaagtac tattgggcat taatttatat gttaaagatt agtagtaaaa
     1741 ataagttagg tgagaaacaa acacatattt tagttgctat aaaaaatata gattatctta
     1801 tgcaactaca taaaaaccaa aaagatacat taacaggact atacaattat gaaagttttt
     1861 gtgaacaaat aagtttaatg cttaaaagat atcctgacaa gcgttatgca attataatga
     1921 tggatataga taagttcaag gtaattaatg acatttatgg agttagagga ggagataaag
     1981 ctttaatata tgttgcagag ttgattcaaa aaaacattgg ttctaatggt atatgttgtc
     2041 gtatgtatgc agatgtattt tgcatttttt atgagagtta ctctgataga gatattcata
     2101 aggtaattta taagattaaa aatagcttaa atagaaaaaa atttgaatat atgttagtac
     2161 catgttttgg agtttataga attttggata atcatattcc aattacaaat gcgtgtgaaa
     2221 tggcaggata tgctcacaaa aaagtaaaga aacaaagtat caacaactat atattttatg
     2281 atgatactat aagaaatgat gcacttgaag aaaaacagat ggaaagggaa atggaatacg
     2341 cacttgaaaa tgggcaattc catgtatatt tacaacctaa gtataatctt aatgcaaata
     2401 aaattgttgg tgcagaagca ttggttagat gggttcatcc agaaaaaggt ataatacctc
     2461 caattaaatt tatttcacta tttgagaaga atgggtttat tatcagatta gatatgtatg
     2521 tatgggaaca agtatgttct ttgattagaa aatggataga tttaggtgaa gagccagttc
     2581 ctatctctgt aaatgtatca agattacatt tgcataatcc acattttaaa gaaattgttt
     2641 taagtttaat aaataaatat agtataccaa aatctttttt agaattagag cttacagaaa
     2701 gtttatttat taaaaatata aaattattta ctaagataat agccgacctt agaatggatg
     2761 gtattgtttt aaatatggat gactttggtt cagcatattc ttcattaaat atgttaaaaa
     2821 atattaatat tgatacatta aagatagatt gtggtttctt taatgaggaa ggaatcacag
     2881 acagagaaaa aatagtttta aagcacacta tagccatggc aaaagattta gatatggatg
     2941 tagtggcaga aggtgtagag acaaaagaac aagctgaatt tttagtgagt ttagattgtg
     3001 tggtaattca aggttattat tattgtaagc ctattccaag tgctgatttt gagaagttac
     3061 tttatcaaaa ataagctaat tattccattc taaatgtagg taggatggat tagttcaaaa
     3121 taattaaata gaagtagaaa atagtgagga tataattttt aaaaagtata atatttatgg
     3181 attaggagcg aagtttatga tagtgggtat tataggtcct tcagactctg ggtttaagat
     3241 aaaggatgat ttaaaacaga tagacagtga tttaaagact aaaatatatg taagagaaaa
     3301 agtagtagat acaatagaag ttatttctaa atgtgaagac gaatgtgatg ctattatatt
     3361 tacaggatgt gcagtttatg aatttataaa atctaagtat gagataagta agccacattc
     3421 atttgtaccg agaagtggga cgagtataat gaaggcattt tgggcaataa aaagtgcaaa
     3481 tataaaatta gataaattca gtatagatgt agttgataga tatgttgtgg aagatgcact
     3541 taaagaattt gatattaaag caacgagtgt attttgtaat ccattttctt tggaagtagg
     3601 agagtctgaa cttgtagaat ggcatataaa attatttgaa gaaaataaga cggatataat
     3661 gttaactggt tttggtgcag tatataatga attaaaagaa agaggctatc ctgtatttcg
     3721 tttacaagct actatacaat taatcaaaga aagttatgac aaggtaaaga gtgaatatgc
     3781 tctaagtaag gcaagatttt ctcaaattgc tgttgaaata ttgagtttaa tagattataa
     3841 agagaagata gataactatt attctgacat gataaaaaaa tcagatatgg ataagcttgt
     3901 agtaaactat gtaagaagca ttcaaggttc cttattttta cttggaagaa atgattatgt
     3961 tgtatttgtt cataaagggg tagctgataa tgagtataat tatgataagc tattcaatct
     4021 aaagagagag ataaaagaca tgggcttttc tcttagtata ggaattggta ctggagtaac
     4081 tgcgtatcag gctgaaaata atgcacataa agcattaaga cactccatag attcaaaaga
     4141 aattggtata tatttggtag atgaagatga aaacataaga ggacctttag cttccgaaaa
     4201 tgaattaaat tattctttga tgctatctga tgaaaaggta atcgaaatat ctaaaaagac
     4261 tggaatgagt tgtgagtcag ttgcaaaaat tattgctata aatgagaata gaaaaagtaa
     4321 gatttatgat tcaaaagagt tagcagagta tctaaatgta agtgaaagaa gtgcaagacg
     4381 tatattaaat aaaatagtaa atgcaggttt aggtcgaata tgtgcaaaag agactagtat
     4441 tggtggagga agaccaaaaa atattactga aatactgttt taagatattt tataaaactg
     4501 tataaatcat tatattgtat aaaattctac aacaaattac tatataaagt attaaagctt
     4561 attatgaaaa aaattagaac aaataattat gtaaaatact agaataaatt attaatataa
     4621 aatactagag cctattatta aaaatgctag aataaattat tatgcaaaat attgaagctt
     4681 attatgaaaa atgttagaac aaattactat ataaaatatc aaagcttatt atgaaaaatg
     4741 ttaaaacaaa ttattatata aaaatactaa aacctatgta aaacaactct aatatataac
     4801 ttataaagtt gaggatacat aggtttttat ttttataatt taaagagttc tattatgata
     4861 tttataattt aatcatatga taaactattt tggcagtatc atataattcg tctatattta
     4921 catattcatt aactgtgtgt acatcataca tacctagact taatatagca caattatatc
     4981 caagattagc taatatattt gcgtcacttc caccaccaat taccattgga tttggaacaa
     5041 caccaacttg tcttattgct tcttcactta acttgaaaac atgactatct ctactaagtt
     5101 caaaactagg gtaagacata ttatgttcaa aagtataagt agtattaaat ttagatgcag
     5161 cgtccttaca acatttttcc atatgattaa gctcatactc taaagtttca ggtatatgag
     5221 acctaatttc agcagtcaat gtaaccttat cagttactat gtttgtagct ccaccacctt
     5281 ctatttcacc tatatttgaa gtagtcaatg aatctattct gcctatgtgc atgttgctta
     5341 ttgcatgaga ggctactaat atagcattta accctttttc aggttctata ccagcatgtg
     5401 ctttctttcc ttgaaaagag atttttatag attgttgagc aggagctttg tatgctatcg
     5461 aaccaatagc tccaccagaa tctaaaatca ccatatcttt acaaggcaag ttgtcaggat
     5521 taaagttttt gacaccatgc ataccagctt cctcacagat tgtaaaacaa agataaatat
     5581 ctctatgtgg tattttttct gtaataacat gttctaaagc ttcaagtata gcagctattc
     5641 cagctttatc atctgcacca agtgtagtag ttccatcact tctaactaca ttaccatcta
     5701 gtattggttt tacacctaaa caaggttgaa cttggtccat gtgtgcacat aaacaaagtg
     5761 gtctacttcc ttcttcacct tttatatagg caatgacatt acctgtatta ccaccatatt
     5821 tttcacctgc atcatctata atggcatcta tatttctttc ttttaagtag tttaccaacc
     5881 acttagacat ttctaattct tgattagatg ggctatcaat tctaaccata tccataaaat
     5941 tatttataag tctattttta tctaacataa attatttctc cttttagaaa gatttaaata
     6001 aattacatgc tatatgaagg aagtattcca ggtcctagtg gaatattaaa taccataaac
     6061 aatattagta gtatagtcca cactattaag aatgatatag agtatggtat catattagca
     6121 attacagtac ccataccagc atctttatcg tattttcttg tgaatgctag tataataggg
     6181 aagtacggaa ataatgggct aagtggattt gttatacaat cacctatacg ataagccatc
     6241 tgagtaagtg ctggattgta tccaaccatc ataaacattg gtacaaatat aggtgcaaga
     6301 atagcccact ttgcagatgc actacctata aataaattta taaaacagga taatataata
     6361 aatcctatca acaataaagg tccatttatt cctgctgatt ttaaaaattc tccgcccttt
     6421 acagctagta tcagacctaa attactattt gtgaataatt gcaaaaattg tgaagcagca
     6481 aatgctagta ttatatatcc acccatatca ctcattgcac tagccattat agagactaca
     6541 tccttatcct tctttatttt acctatagtt tttccgtata caacaccagg tatgaaaaat
     6601 gctagtgcta ttataggtac catacctttc ataagaggag cattgtttga tagtaaatct
     6661 cctgtttctg ggtcagctaa gaatggtttt ttaccgatta cactaagagc tactataata
     6721 gccacatata ttaatatact tataccagct tttttaagcc ctttcttttc tacatcacta
     6781 agacttccat caacatctag ttctgcattt ccttcatatt taccaaatct aggagctaca
     6841 attttttctg ttacccatgt tgccaaaagt attagaacaa aagttgatgc tatcataaaa
     6901 aataagttca ttgttgcatt ggcttgaaat ccagaatcaa tcatttgagc tgctggaact
     6961 gtaaaacttg cagcaagtat atcattgaca cttatcataa tgtttgcggc aaatcctaga
     7021 cagactgctg catatccaga aaataaacct ataagtggat gtctaccaac accaagatat
     7081 acaagtgcag ctaatggtgg aaataatatt gtacctgcat ctgaagctat attggcaagc
     7141 atacctatag tcattacaac aagagttact cttgtttcag gaatcttgct tactgatgct
     7201 ttgattgttg ctgtcataag tcctgttttg tcacatacac cagcacctat catagttact
     7261 agaactagac ccaaaggagc aaacccttgg aaattgctga ctatgtttcc taaaagaatt
     7321 tgaagctgtt ctacagtcaa taaatttaca gcggttatag tttctccagt accaggatga
     7381 actactgata tccccataga accaactaga ctagatagta taagtaaaag tacacataac
     7441 cctaaaaata ttgtaactgg gtctggtagt ttattaccaa cagtttctat tctgtttaat
     7501 gtcctgtcaa aaaagttttt cttcttaact acgttatcat tcataatatc ctccctaatg
     7561 tttaaaaata taattaaaaa ataagtattg tggttaatct tacaatttaa attctgcttg
     7621 tcgatttaag acgtgtccac aatatgtcct aaaaaaagta taaaataaac tttttcctgt
     7681 gtcaatgcag ttttctgaat tttctggttg agtggtgtta gttaaatatg ctttaagtag
     7741 attaatgacg aattatatct aatgtattta aataacatat gcgctttaaa attaatttta
     7801 aaacaaaatt ttatgaaaag ctagtggaag attaagttat taaaataagt agcgtagtaa
     7861 agtgttagat tttttaatta atcaaagaaa tttactttgt attttctaca aaaatctgtg
     7921 aatatctttt gaattatgta gaggatataa atttatttta tttacataga ttagattttg
     7981 ttctaattta ctgtattcaa atggttttcc atgtccagga taaatcattt taggttttgt
     8041 attgataatt gttttccaag attgaataaa ttctagtttg ctttctgcaa atatggtaat
     8101 tttgtgagta cttggaaatc cattcattgc agaatctcca caaaataaaa caccatcatc
     8161 aattagtagt gaaattgaat catcagtatg tcctggtgta tctataattt ttccatttag
     8221 ttctttttca atttctttac gattcttgtc agacacaaga atacaccttt tttcaaattc
     8281 ttgtttaagt ggagaaaaac tatgctcacc ttttccaaat agtttcataa tttggcaaaa
     8341 gagaaaggca gtacggctgg tacaaccacc aataaaagag ttttgacctt tatatagaat
     8401 ttctaaagct ttatcatgca taacaatctt tgtattagga gattcgtcta gaatctgatt
     8461 tagaaaacca acatggtcat catgagcatg agttagaaat atgtatcgaa tatctttgat
     8521 ttttatgttg aatttagaaa ttcgtttttt gaaatgttta aaaccatttt catatccagt
     8581 atcaatcaag acaaagccat cttgaatagg gtacaaccat gtatttataa ttcgatttcc
     8641 tgcattaaac attttttcac caccttgaat tagaatagaa atttatatta attagaaaga
     8701 atatagcata gttctactga tatttcaacg tagctctaaa agttttaagg aggataatat
     8761 attttagttt aatctaaaaa ctaaaagaaa agaagtatac tgttacaaaa acaaagagtg
     8821 gtataacttt tacagtagat accaccctat tataaaaata gacttataaa gttttatcgt
     8881 gtttttctaa gaatgttaga tattcttttt tagtcataac tggagtaaag gtatctaata
     8941 tatctttagc atccttagca ttatcaaaaa gaaggtctac aactgtaagt gccattgctt
     9001 tagcaggaat tatgtaagct aaatcttcat caactatttc aaaatctctt gtatgaagag
     9061 ctccttttac accaccaatc atagggtgaa gagtaggcat tatatgagac acatctccaa
     9121 aatcaaaaga gcctgtaaaa tctccaccat caataacttt tccttttaga cctaaatctt
     9181 caagattgtt tttaaataaa ttatcaagac ttttgtatcg taatattgga agatatccag
     9241 gaatttcagt tatctcaaca tcagctccaa ctgccattcc accagccatc aaagccttat
     9301 ttattctttc attagcatca atcataccat ttatagttct agctctaaca tatgattcca
     9361 tatgaacatc agcaggaaca acatttacta tatctccacc ttttgtgatg atagggtgga
     9421 atctaactct ttcagactct ttaaaagttt ctctaagtgc attcacgttg tttatagcaa
     9481 gcattgctgc atttaaagca tttattccat catgtggagc agaaccagca tgagattctt
     9541 tacctataaa tttaaccttt tttccaacaa atccattact ctcaggacca actagagcct
     9601 tatcttctcc taaatcagaa gcatgaaaca tcatagatat gtctatatca tcaaaataac
     9661 ctctttttat taattcttgt tttccaccaa aataagttat ttttccttct ttttttaatt
     9721 gctctctgta ttccaactca ataaactctt cagcaggagt agccataaaa gatactttac
     9781 catctaaatg ttctaaaacg ccacttttta ctaaaccaac agcagcacct aacattccag
     9841 ctatttgaat attatgtcca caagtatgag aagcacctat ttcattagca tctttatgtt
     9901 ctttacatga tataccatcc aattctccaa gtatagatat atgaggacct ttcttgctag
     9961 aatttgcatc tgctttgcat cctgtaactg ctatattttt ttctacagat aaatttaatt
    10021 cttgctcaaa aaagtttgaa acagcttctg tagttttaaa ttctttatat ccatattctg
    10081 gatttttgta tattccttga cctattttta atattttttc tctattttca tctatagttt
    10141 ttattactag agattttaat tgttctttat tcataataaa ttcctcctat attttacctt
    10201 gaatacttaa aactatatga gctatactcg cacatgcaaa gaatatagtt gtaaatacaa
    10261 taagtgaaat tataactatt ttccatgaca tttcttttaa agcttctatt ttgtttccaa
    10321 cagaaattcc tgcaaaagct agtagtggag tagttatagc cataaagtta atattattag
    10381 tgttagttaa gaatacttca gatgttggac tatatggcat actaagtaaa aggccgatta
    10441 tagttgcaaa tccaaaagct ggaaagattg atttaggaaa taaatctttt ataatcatag
    10501 ctgccatagc agctaatatt agcatcacca taccaggaag agctgtaact atagatgtac
    10561 caacgctaat tctttgtcct ataagaatta taactccaat aacagacata accaataatt
    10621 gatatgaact tttctttaaa tttaaattag caaacattta ttagcactct ccttctgtag
    10681 atttaatttc tttagttttt ttatttctcg aaaattttgg ttctaaaatc ttatacataa
    10741 aattagtaaa tggtaaggat acaaaaacta agaatataat tcctgttata ctagacatca
    10801 tattgcttgt tgcagcatag gcaagtattg tatctgccat ttcaggtggt accatggtag
    10861 ataatgaacc agcagctgct gtcatcatac ttccactacc aacaccacag gccattccca
    10921 aagcatatgg gtgaagacca ctataaatag atattgaacc tagtacacta aagtatatag
    10981 taccaagaac tgttcccatt aaatatgtac caagtacacc actaccttct ggtgagttta
    11041 tcccatattt ttctgatata actcctaaag aaggttcacg acttatactt gcagtagctc
    11101 caacagcttc tcttttcatt ccaaacatta atgcaagagg taatgcaacg attggtgcaa
    11161 gtatatgacc aaattcttga gcgataaacg ctggtccagc ttgaattatt ttgtcaatgt
    11221 ttggtccaac tgtagtacca tattttatac caagcattac tagtgcaatt ccaacaactt
    11281 caccagcaat atttatttct ttttctccaa ccactttttt aagtgcttta atttttttac
    11341 ctaataaatc tggtgttatt ataagtccaa ttataactgc ataaagcata gggaacagta
    11401 ctatagaggc ttttccaata gctatagttc tttgccctat aaattcacat attactacca
    11461 agattaagca agttaaaaat acttgaataa aatgtttttt attaatttcc atgatttcct
    11521 ccccaaaaga ataaatattt tacttatctg aaatgagaaa atacgttttc ttaagtataa
    11581 tacgttttcc taaataaaat gaaaaaacta tttcaaataa gtttcagaaa tgtcaataac
    11641 atgtccttaa ttaaggataa aacaaaaatt taacattgtc aacgaaaaat atctaaaata
    11701 ttacatattt tgaatatatt gtaatatagc tttattatga aaaaattg
//
LOCUS       DAELLV010000113        11742 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.113, whole genome shotgun sequence.
ACCESSION   DAELLV010000113 DAELLV010000000
VERSION     DAELLV010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11742
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.113"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            104..1144
                     /locus_tag="KPD58_002292"
     CDS             104..1144
                     /locus_tag="KPD58_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0266054.1"
                     /translation="MDKHSDSVMYGGMEVYNIWGTTTVDSIIGESDVMRALKNRICKI
                     GNSDSTVAITGESGTGKELIARAIHLAGNRGNKTFVAINCAAIPEPLLESELFGYTKG
                     AFSGADSKGKIGKFELANGGVVFLDEIGDMPIQLQSKLLRVLQEKKFTRIGSNELIDI
                     DVRIISATNRDLFELVKENKFREDLYYRLNVIPLEVPPLRERGDDIQILINNFIDKYS
                     RKMKKNVYHIEPDVEQMLLKYPWPGNIRELENTIELALNLLEEDGIIHKGIINRKILE
                     YFKLNNINIKLLEESDYEISMEQDILTLEEFERAYIKKVLKFYGNDTKSKKLAAKKLG
                     ISLATLYRKIDV"
     gene            1472..2818
                     /locus_tag="KPD58_002293"
     CDS             1472..2818
                     /locus_tag="KPD58_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBG0266055.1"
                     /translation="MSKYQKNVSMFDIDGRPPILKCLPLSMQHILAMIVGTVTVPIVV
                     GGAVGVSPTQITLLVQYALIIAGISTLIQLYPIGNIGSRLPIIFGVGFTYVPTLVAVG
                     GNYGLASILGAQLIGGVATVLIGIFIKKIRKFFPNIVAGTVVFTIGLSLYPIAINYMA
                     GGVNSPTYASMQNWAVAAITLVVVIGLSQFAKGYAKLAAIFLGIIVGYCVSLVLGIIN
                     FDPIREATWFAIPKPLEFGMEFDMPVIISVVIVSIINAVQAIGDLSATTMGGMDRNIT
                     DKELSSGVIGSGICTMIGALFGGLPSSYSQNVGMVAMNKVISRFVLGIAGIFMLLSGF
                     VPKFGALMTTIPYSVLGGATISIFSMITMTGIKLIITEELSSRNITIVGLAIALGMGI
                     TSVPAAQEAFPSFVKLVFGESPIVIATLVAFVLNLIIPNKSLEDEAKERAVMEETN"
     gene            3115..4476
                     /locus_tag="KPD58_002294"
     CDS             3115..4476
                     /locus_tag="KPD58_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HBG0266056.1"
                     /translation="MKTRNTSKYDVDGIPPLKESIPLALQHLLAMIVGNMVPAILIAN
                     VVGLNQGQATMLIQGSMLAAGLATFLQLYPIPLFKGFKLGSRLPVMMGMSYVFLGACL
                     SVAAKDGLAALFGAQIAAGVIVFFVGFGVKKIRHIFTPIVSGTIIACMGLGLFATAIK
                     NLAGGEGTQTFGSPINFTVGVIVAFVIIMINKYGKGLVKNSSILIGILVGYAISLVLG
                     LVDFSAVQGAAIVSLPTPAAFGLEFRPELIVMFTIIYIIGIADMMGACTIATVGAMDR
                     EVTDEELASVVLGNSITSIISSLFAALPTGVFSQNTVIVSMNKVTSRFVIALGALVLL
                     LAGISPALGAIMTTIPSCVVGGATLVVFSSIAMSGFSIMSMDGFTEENNLIAGVSIAT
                     SMGLTTAPQVLDQFPETIGTVLGGSSIVSGAIIAILLQTLFKLKSRKSAENVTSNLEE
                     NIG"
     gene            4549..5943
                     /locus_tag="KPD58_002295"
     CDS             4549..5943
                     /locus_tag="KPD58_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0266057.1"
                     /translation="MIDILIKNAIIVTVNKDRDVIFDGAIAIQGDRILDIGNTVDIEP
                     KYLDAKKVIDANGKVIFPGFINTHNHLFQVLLKGLGDDMALHEWLNTMMFPSAKFLTE
                     QDTYDAAMLGCMEGLKSGITTMVDYMHTHNRPGLTDGIVKAYKDLGIRGVVGRGCIDL
                     GIHKELIEDVETVEKDLRRVFEKYHNSENGRIKICVAPSSMWAISEEMGKMLWNIVKE
                     YDSYFTVHISETEFARTATKDIHGEIDIKLLEKWGIVGPEVVAVHCVCITDEDMEMLK
                     KYDIKVSHNVASNMYLASGVAPVPEMLKKGINVSLGLDGAASNNAQDMVELMKLTALQ
                     HKVNNCDPLAISAEKVLEMATIEGARTLRMEDEIGSLEIGKKADLVIFNSMLSPKSIP
                     LHNPVSTLVYSASMHNVESVLVDGNVILENGKVTTIESEEQVLLNAQHAAEELCKRAN
                     VTNRMEGHKWNSLY"
     gene            6005..6475
                     /locus_tag="KPD58_002296"
     CDS             6005..6475
                     /locus_tag="KPD58_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HBG0266058.1"
                     /translation="MEKIKLNINDYMYEIEVEKNETLLHVLREKLNLTGAKQGCNSGS
                     CGACKVLIDGVDMKSCKLLAKNCEGKVIYTIESFANGENLHPIQEAFIEVGAVQCGYC
                     TPGMIITTQALLNRNASPNEDEIREAFKENLCRCTGYVKIIEAVKLSAARLGGR"
     gene            6478..8694
                     /locus_tag="KPD58_002297"
     CDS             6478..8694
                     /locus_tag="KPD58_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG0266059.1"
                     /translation="MEKVIGKSYPLKDAALKVTGQMKYVADMKPQNVLYAKMLLSPVA
                     HAKIKSIDTSEAEKVVGVRAIATYLNTSQRKYNGSLRFYEHKIPENEVIFSDTVRFVG
                     DRVAAVAAETPEIAEKAVKLIKVEYEELPAVFTIQDAIKEDAICIHGDSNIVSQNNIN
                     GGDVEKGFKECDYIFEDEYSTPAIHHSAIETHSVIAHYDYNDKLTVHTPCQNTFGFRI
                     ILSQIFGMSLNKVRVIRPAIGGSFGGKLELTIEPVAAALSKMTRRPVKMVLTRKESMI
                     STRTRHGSFVKLKTGVNKDGEVIAQDIKIYTNTGAYASSALNVIGALSHKVFKVYKIP
                     NIKFTGMPVYTNTPIAGAMRGYGSPQIFMAQQAQFAKIAKEIGMDLVDFQNKNAVEPD
                     DVDIIFHGSLGNPRVLDCIEQGKKMFKWDEKKKLPKEEGKYLRGIGMAIGAHGNGVFG
                     AHRDVVTLTLKLNEDGTLTLLTGAHDMGNGVVTMQTMMVAEVLGITPDKVDTFETDTD
                     ACTFNLGDYASRGVFVEGGGAKKTAEKLKNLILEEAEKLLETSKEDLYLDNGCVISKL
                     DENVKASLSDVAVYSQSKSLRELTVVEDYSSPAGRTSYGVHFAEVLVDKETKDIKVVD
                     FVAVHDVGRVINPLNLEGQLEGGIHMGLGYALSEELAFDEQGRPKATDFKTYKLFRTV
                     DMPKCQVAFVDGYEKAGPFGGKSIGECAVVPVAPAVANAVYNATDVEIHSLPIKLK"
     gene            8777..9448
                     /locus_tag="KPD58_002298"
     CDS             8777..9448
                     /locus_tag="KPD58_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG0266060.1"
                     /translation="MAKILGICGSPRKGATEYAVLEALKEAETIPGIETEYWSVRGKK
                     ISPCVHCDACIRKETMCIIKDDIQELEQKILEADGIIVGSPVYDMNITAQLTAVFNRL
                     RPMYLVHPGKLQNKVGAAITTGGTRYGGQELTKLPIINFFLMHEMLVSGGLGGCYIGG
                     TIWSKDGKAKGAQEDETGMDTVKRLGKGAAEAVMVSKFGLEKWNVVKEELDVKKDEKS
                     PLRDH"
     gene            9811..11025
                     /gene="dpaL"
                     /locus_tag="KPD58_002299"
     CDS             9811..11025
                     /gene="dpaL"
                     /locus_tag="KPD58_002299"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBG0266061.1"
                     /translation="MLKEIKWKVNNLPKGDKENCIKFLNEEEITKVRNFHKSFPQYKE
                     TPLANLEGLAKKLGVAGVYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAQEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNHAAAFVSAPDWVSAKGMRTLGNP
                     LNGDEKVISGESGAVTTGLLVAAMEREDLADLRKDLKLDENSRILLISTEGDTDPDKY
                     RSIVWDGEYPSI"
     gene            11152..>11742
                     /locus_tag="KPD58_002300"
     CDS             11152..>11742
                     /locus_tag="KPD58_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBG0266062.1"
                     /translation="MLNEQRKQEVTEICQKLIQNPSSSGNEEGVVKAIEESFEKLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEGKIVDGKIYGRG
                     TSDMKGQYTAMMSAVAYFAKDTNRDFAGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKG"
BASE COUNT         4438 a         1354 c         2389 g         3561 t
ORIGIN      
        1 aaataatatt attaaattat aactaagctt ttctgttatg atataattaa atagataatg
       61 tgaatgatta ttatatagaa tacttatttg gagttgatat tttatggaca aacacagtga
      121 ttcagtaatg tatggtggaa tggaagtata taatatttgg ggaacaacaa cagtagactc
      181 cataattgga gagtctgatg taatgcgtgc tcttaagaat agaatttgta aaattggaaa
      241 tagtgattct actgtagcta taactggaga aagtggcact ggaaaagaat taatagcaag
      301 agccattcat ctcgcaggaa acagagggaa taaaacattc gttgctataa actgtgctgc
      361 tataccagaa cctttactag aaagtgaact ttttggatat acaaaaggag cttttagtgg
      421 agcagattca aaaggtaaaa ttggtaagtt tgaattggcc aatggagggg ttgtattttt
      481 agatgagata ggagatatgc caattcaact acaatcaaag ttattgaggg tattacagga
      541 aaagaagttt acgagaatag gttcaaatga actaattgat atagatgtta gaatcatatc
      601 agctactaat agagatttat ttgagttggt taaggaaaat aaatttagag aagacttata
      661 ctatagattg aatgtaatac ctttagaagt gcctccactt agagaaagag gagacgatat
      721 acaaatatta ataaacaatt ttatagacaa atattcgaga aagatgaaga aaaacgtgta
      781 ccatatagag ccagatgtgg aacaaatgtt gcttaagtat ccttggccag gaaatattag
      841 agagttggaa aatactatag aactggcatt aaaccttctt gaggaagatg gaataataca
      901 taaaggaata ataaatagaa agatacttga atattttaag ttaaataata taaatataaa
      961 gttacttgag gaaagtgact atgagataag tatggaacaa gacatactta cacttgaaga
     1021 gtttgaaaga gcctatataa agaaagtgtt aaagttttac ggaaatgata ctaagtctaa
     1081 aaaactggct gccaaaaaac ttggaatatc actagctaca ttgtacagaa aaatagatgt
     1141 ataataatta ggctttttat tctcaattct aaaatttgaa attataaaat tgataaaaaa
     1201 cacttggaaa actattttct aagtgttttt ttgtgtaata gcattttatt atccaaaaat
     1261 aaaaaaaatt taaaagaatt taaaaaaata aatgctttaa taaattatca aatactaaca
     1321 aatataataa aaattatata tgtaaaacaa ttatgttaaa aaattggcat tataattgct
     1381 tcatattaag ttgagatgac aaaataaaaa aagaaaggat gttgtgtgag ataataataa
     1441 ctgtcattaa aaatagttgg gagtgataac cttgagtaaa tatcaaaaga atgtttctat
     1501 gtttgatatt gatggaaggc caccaatttt aaagtgtttg ccactatcaa tgcaacatat
     1561 actagcaatg atagttggaa ctgttacagt acctatagtc gtaggaggag ctgtgggtgt
     1621 tagccctaca caaataacac ttttagtgca atatgcatta ataatagctg gtatatcaac
     1681 acttatacaa ctatatccaa taggaaatat tggttcaaga ttgcctataa tttttggagt
     1741 tggttttact tatgtaccaa cacttgtagc agtcggagga aattatggat tagcaagcat
     1801 tttgggtgca caattaatag ggggagtggc tacagtatta atagggatat tcataaaaaa
     1861 gataagaaaa ttttttccga atatagttgc aggaacagtg gtttttacaa ttggattatc
     1921 tttgtatcct atagcaataa attatatggc aggtggtgta aatagtccta cttatgcctc
     1981 tatgcaaaac tgggcagtag cggctataac attagtggta gttataggtc ttagtcaatt
     2041 tgcaaaagga tatgcaaaat tggcagcaat atttttggga attatagttg gatattgtgt
     2101 atctttagtt ctgggtataa taaattttga ccctattaga gaggcaactt ggtttgcgat
     2161 acctaaaccg cttgaatttg ggatggaatt tgatatgcct gtaataatct cagtagtaat
     2221 tgtaagtata atcaatgctg ttcaagcaat tggagactta tcagctacta caatgggagg
     2281 catggatagg aatatcacag ataaggaatt atcaagtgga gtaataggaa gtggaatttg
     2341 tactatgata ggagctttgt ttggaggttt accatcatca tatagccaaa acgtggggat
     2401 ggtagctatg aacaaagtaa taagtagatt tgtgcttgga atagctggta tatttatgtt
     2461 attgtcgggg tttgtgccta aatttggtgc attaatgact actataccat attctgtttt
     2521 aggaggagct acaatatcta tatttagtat gataacaatg acaggtataa agttaattat
     2581 tacagaagag ttgtcatcaa gaaatattac cattgttggt ttggcaattg ccttaggcat
     2641 gggaataaca tcagtcccag cagcccaaga ggcatttcct tcatttgtaa agttagtatt
     2701 tggagaatct ccaatagtaa ttgcaacatt ggttgcattc gttttaaatc taataatacc
     2761 aaataaaagt ctagaggatg aggctaaaga aagagctgtt atggaagaga ctaattaaga
     2821 tataataatt taaaagaatt gtttaaatta tttagtatga gattgttaga ttatttttga
     2881 tagctattca ttttaataaa aaatttaaca aataaatata taccaggcaa ttaatgttca
     2941 tttaagttga aaatcgggga ggcaatattt agaaatttgt taatttttta taaaaatagc
     3001 tcagtttagc tgtgtttatc ggttgagcaa taaattcatg gtcataaaaa tatttataga
     3061 aaataattat aaaaatattt agtcaagata tttatgtatg ggaggattta ttttatgaaa
     3121 acacgtaata cttcaaaata tgatgtcgat gggataccac cactaaagga atcaatacct
     3181 ttggccttac aacatctgtt agcaatgata gtaggaaata tggtacctgc gatattaata
     3241 gcaaacgtgg taggacttaa ccaggggcaa gcaacaatgt tgattcaggg ttctatgtta
     3301 gcagcaggtt tagcaacgtt tttacagtta tatccaatac ctttatttaa aggatttaag
     3361 ttaggttcaa gattacctgt aatgatggga atgagttatg tatttttagg agcttgtctt
     3421 tcagtagcag ctaaagatgg tttagcagct ctatttggag cacagattgc tgctggggta
     3481 atcgtattct ttgtaggatt tggagttaag aaaattagac atatattcac tccaatagtt
     3541 tctggaacta taatagcatg tatgggactt ggactattcg caacagctat aaagaattta
     3601 gctggaggag aaggcacaca aacatttgga agcccaataa acttcacagt aggagttata
     3661 gttgcttttg taataataat gataaataaa tacggtaaag gtttagtaaa aaattcttca
     3721 atattaatag gaatattagt aggttatgct atatctttag ttttaggatt ggttgatttc
     3781 tcagccgttc aaggagcagc tatagtatct ttaccaacac cagcagcttt tggattagaa
     3841 tttagaccag agttaatagt aatgtttact attatttaca taataggtat agctgatatg
     3901 atgggtgcct gtacaatagc tacagtagga gcaatggata gagaggtaac tgacgaagaa
     3961 ttagcatcag ttgtattagg aaattctata acttctataa tttcatcact atttgcagct
     4021 cttccaactg gagtatttag ccaaaataca gtaattgtat ctatgaacaa agttacaagt
     4081 agatttgtaa tagcactagg ggcacttgta ttattactag cagggatatc tccagcattg
     4141 ggtgcaataa tgacaactat accatcttgt gttgttggtg gagcaacttt agtagttttc
     4201 tcatcaatag caatgtctgg tttctctatt atgagtatgg atggattcac tgaagaaaac
     4261 aatcttatag caggtgtatc tatagcaaca agtatgggtc ttacaactgc tccacaagtt
     4321 ttagaccaat tcccagaaac aattggaact gtccttggag ggtcaagtat agtttctgga
     4381 gcaataatag ctatattact tcaaacatta tttaaattaa agtcacgtaa aagtgctgaa
     4441 aacgttactt caaatcttga ggaaaatatt ggataaatgt attagatata atatgagaaa
     4501 accatatggt aaaatgttgt aatgaaagta ttcaaagggg gatttaagat gattgatata
     4561 ctaataaaaa atgcaataat tgttacagtt aataaagaca gagatgttat atttgatgga
     4621 gcaatagcta ttcaaggtga cagaatcttg gacataggaa atacagttga tattgagcct
     4681 aagtatcttg atgctaaaaa agttatagat gctaatggaa aagtaatatt ccctggcttt
     4741 ataaatactc ataatcacct gttccaagtg ttattaaaag gtttaggtga tgatatggca
     4801 cttcatgagt ggttaaatac aatgatgttt ccatcagcaa aattcttaac agaacaagat
     4861 acttatgatg ctgcaatgct tggatgtatg gaaggtttaa aaagtggtat aactacaatg
     4921 gttgattata tgcatactca caatagacca ggacttacag atggaatagt aaaggcatat
     4981 aaagatttag gtataagagg ggttgtaggt agaggttgta tagatttagg tattcacaaa
     5041 gagcttatag aagatgtgga aacggttgaa aaagatttga gaagggtatt cgaaaaatat
     5101 cataattcag aaaatggaag aataaaaatc tgcgtagctc cttcttcaat gtgggctata
     5161 tctgaagaaa tgggtaagat gctttggaat atagttaaag aatatgattc atattttaca
     5221 gttcatatat ctgaaacaga atttgcaaga acagcaacaa aggatataca tggagaaata
     5281 gatataaaat tacttgaaaa atggggtata gtaggtccag aagttgtagc agtacattgt
     5341 gtatgtataa ctgatgaaga catggaaatg cttaaaaagt atgacataaa agtttctcat
     5401 aatgtagcaa gtaacatgta tcttgcttct ggtgttgcac ctgtgccaga aatgttaaaa
     5461 aaaggtataa atgtgagttt aggtcttgac ggtgcagcaa gtaataacgc acaagatatg
     5521 gtagagctta tgaaattaac agctttacaa cacaaagtaa ataattgtga ccctctggca
     5581 atttctgcag agaaagttct tgaaatggct acaattgagg gagcaagaac tcttagaatg
     5641 gaagatgaaa taggttcttt ggagattggg aaaaaagctg acctagtaat atttaactca
     5701 atgctttctc caaaatcaat acctcttcac aatcctgtat caactttggt atactcagct
     5761 agtatgcaca atgtagagag tgttttagtt gatggaaatg taatacttga aaatggaaaa
     5821 gtaactacaa ttgaaagtga agaacaagtg ttgcttaatg cacaacatgc agcagaagaa
     5881 ttatgtaaga gagctaatgt aactaataga atggaaggac ataagtggaa tagcttatac
     5941 taatattaaa ataagattgt aataatattc tattacaaca ttagtttatc tgtaggagga
     6001 gaaaatggaa aaaataaaat taaatattaa tgattacatg tatgaaatag aagttgaaaa
     6061 gaatgaaact cttttgcatg tacttcgtga aaaattaaat ctaactggag cgaaacaagg
     6121 ttgtaatagt ggcagttgtg gagcatgtaa agttttgata gatggagttg acatgaaatc
     6181 ttgtaaactt cttgcaaaaa actgcgaagg taaagttata tacactatag aaagttttgc
     6241 taatggagaa aatcttcatc caatacaaga agcttttata gaagtcggag ctgtacaatg
     6301 tggatattgt actcctggta tgattataac aacacaagct cttttaaata gaaatgcatc
     6361 tcctaatgaa gatgaaatta gagaagcttt taaagaaaat ctctgtagat gcacaggata
     6421 tgtaaagatt atagaagctg taaaattatc tgcagctaga ttagggggta gataattatg
     6481 gaaaaggtaa taggaaaatc ataccctcta aaagatgcag ctttaaaagt tactggtcaa
     6541 atgaaatacg tggctgatat gaaaccacaa aatgtacttt acgctaagat gcttttaagc
     6601 cctgtagcac atgcaaaaat aaaaagtata gatacaagtg aagctgagaa agtagtaggt
     6661 gttagagcta tagctactta tctcaataca tcacaaagaa aatacaatgg ttcattaaga
     6721 ttttatgagc acaaaatacc tgaaaatgaa gttatatttt cagatacagt tagatttgtt
     6781 ggtgaccgtg ttgcagcagt agctgcagag actccagaaa tagcagagaa agctgtaaaa
     6841 cttataaaag ttgaatatga agaattgcca gctgtattta ctatacaaga tgcaataaaa
     6901 gaagatgcta tctgcataca tggagattca aatatagttt ctcaaaacaa cataaatggg
     6961 ggagatgtag aaaaaggctt caaggaatgt gattatatat ttgaagatga atactctact
     7021 ccagcaatac atcatagtgc aatagagact catagtgtaa ttgcacacta tgattataat
     7081 gataaattaa ctgtacatac accttgccaa aatacatttg gatttagaat aatactatct
     7141 caaatttttg ggatgtcatt aaataaagta agagttataa gacctgctat tggaggctca
     7201 tttggtggaa aattagaact tacaattgaa ccagtagcag ccgcattatc aaaaatgaca
     7261 agaagaccag ttaaaatggt tcttacaaga aaggaatcta tgatatcaac aagaactcgt
     7321 catgggtctt ttgtaaaatt aaaaactggt gtaaataaag atggtgaagt aatagcacaa
     7381 gacataaaaa tatatacaaa tacaggagca tatgcatcaa gtgctttaaa tgttatagga
     7441 gcacttagtc ataaagtatt taaagtttat aaaataccaa atataaagtt tactgggatg
     7501 cctgtatata caaatacacc aatagcagga gctatgagag gctatgggtc acctcaaata
     7561 tttatggctc aacaagctca atttgcgaaa atagcaaagg aaataggtat ggatttagtc
     7621 gattttcaaa ataaaaatgc agtagagcca gatgatgtcg atataatctt ccacggttct
     7681 cttggaaatc ctagagtatt ggattgtata gagcaaggta agaagatgtt taagtgggat
     7741 gaaaagaaaa aacttccaaa agaagaagga aaatatttaa gaggtatagg tatggcaata
     7801 ggtgcacatg gtaatggagt ttttggtgca catagagatg ttgttacatt gactcttaaa
     7861 ttaaatgaag atggtacgtt gacactacta acaggagctc atgatatggg taatggtgtt
     7921 gtaactatgc aaacaatgat ggtagctgaa gttttaggaa taacacctga taaagttgat
     7981 acatttgaaa ctgatacgga tgcatgtaca ttcaaccttg gtgactatgc aagtagaggg
     8041 gtatttgtag aaggtggagg agctaaaaag acagcagaga aattgaaaaa tcttatatta
     8101 gaagaagcag aaaaattact tgaaacatca aaagaagatt tatacctaga caatggatgt
     8161 gtaattagta aacttgatga aaatgtaaaa gcatcacttt ctgatgtcgc agtatatagt
     8221 caaagtaaat cattaagaga gttaactgtt gttgaagatt attcttcacc agctggacgt
     8281 acttcttatg gtgtacattt tgctgaagtt ttagtagata aagaaactaa ggatataaaa
     8341 gtagttgatt ttgtagctgt acatgatgtt ggtagagtta taaatcctct aaatttagaa
     8401 ggacaattag aaggtgggat tcatatgggt ctaggatacg cattgagtga agaattggca
     8461 tttgatgaac aaggtagacc taaagctact gattttaaaa catataaatt atttagaact
     8521 gtagatatgc caaaatgtca agttgcattt gtagatggtt atgaaaaagc tgggcctttt
     8581 ggtggtaaga gtattggaga atgtgccgtt gtaccagttg cgccagctgt tgcaaatgca
     8641 gtatataatg caactgatgt agagatacat tcattaccaa taaaattaaa atagtagaaa
     8701 ttaaagcaga aataacaatt tattgtattt gtaaatagat aataaaaata gatttatttg
     8761 gtgaggagat attattatgg caaagatatt aggtatttgt ggaagtccac gtaaaggagc
     8821 aacagaatat gcagtgttag aagctttgaa agaagcagaa actatacctg gtatagaaac
     8881 tgagtattgg tcagttagag ggaaaaaaat aagtccttgt gtacattgtg atgcatgtat
     8941 aagaaaagaa actatgtgta ttataaaaga tgatatccaa gaattagagc aaaaaatatt
     9001 ggaggcagat ggaattatag taggttctcc agtttatgat atgaatataa ctgctcaact
     9061 tacagcagta tttaatagac ttcgtccaat gtatttagtt catccaggaa aacttcaaaa
     9121 taaggttggt gcagctataa ctactggtgg aactagatat ggtggccaag aattgacaaa
     9181 acttcctata ataaatttct tcctaatgca tgaaatgtta gtgtctggag gtttaggtgg
     9241 atgctatata ggtggtacaa tatggagtaa agatggaaaa gcaaaaggtg cccaagagga
     9301 tgagacaggt atggacacag taaaaagact tggaaaaggt gcagcagagg cagttatggt
     9361 ttctaagttt ggtcttgaaa aatggaatgt cgtaaaagaa gaattagatg ttaaaaaaga
     9421 tgaaaaatca cctcttagag accactaaag atttattata ttattgaata tatacaaata
     9481 gtgatgtaat agattattgc atcactattt tatgttttaa tttagatttt ataaatttta
     9541 tattaaaaaa tatagaaaca aagtgttttg agatttattt ttaaatttta aaaatgagaa
     9601 ttaaaaagtg agaagataga aaaggttttt tagttattaa attctacata cattgtatga
     9661 actaaagata gaaaagaaaa aataataaaa gcattgcaat ttctatacta aagagtcatg
     9721 gaaaaatatc tagtatatat ttagtattgg catgttaatt gcttatatat taaagtataa
     9781 aaatttaaca ataatttgga ggtattttag atgttaaaag aaattaaatg gaaagtaaat
     9841 aatttgccaa agggcgataa ggaaaattgt atcaaatttt taaatgaaga agaaataact
     9901 aaggttagaa attttcacaa aagctttcct caatataaag aaactccatt agcaaaccta
     9961 gaaggtttag caaaaaaatt aggtgttgca ggagtatatg taaaagatga atcttataga
    10021 tttggattaa atgcatttaa agtattaggt ggttcttatt ctatgggtag atacttagct
    10081 caaagattag atacagatat atctgagtta gggtatgaca aattgacttc gaaagaaata
    10141 aaagaaaaat taggagaaat aactttcttt acagctacag atggaaatca cggtagagga
    10201 gttgcttgga cagctaataa attaggtcaa aaatctgttg tattaatgcc aaaaggttct
    10261 tctgaattta gattaaataa aattaaagga gaaggtgcag atgctagtat aacagactta
    10321 aattatgatg atgcagtaag attagctaat gattatgcag aagctgatga ccatggagta
    10381 atggttcaag atacagcatg ggacggatat gaagaaatac cagcatggat aatgcaagga
    10441 tatggaacta tggctcaaga agctatagag caattaaaag aatatggagt agatagacca
    10501 acacacgtat ttgttcaagc aggagttggt tcacttgctg gtgcagttca agggtatgtt
    10561 gcttcaatat atgatgaatg cccaataaca gtagtagtag aggctgatga agctgattgt
    10621 tactataaat cagcagaagc tggagatgga aaaccaagat ttgttggagg agatatgcca
    10681 actataatgg cagggcttgc ttgtggagaa ccaaatacta taggatttga agtattaaaa
    10741 aatcatgcag cagcatttgt ttcagcacca gactgggttt ctgctaaagg tatgagaaca
    10801 ttaggtaatc ctttaaatgg tgatgaaaag gtaatttctg gagaatctgg agcagttaca
    10861 actggtcttt tagtagcagc tatggaaaga gaagatttag cagatttaag aaaagattta
    10921 aaattagatg aaaattcaag aatactttta ataagcacag aaggagatac tgaccctgac
    10981 aaatatagaa gtatagtttg ggatggagaa tatccaagta tataaggaat tactaaaatg
    11041 caattattag atattggttt acatatttag aagatatata agttctaaaa tttagaaaat
    11101 atataagtat ataaaataca tacattataa aaaatctagg gggataatga aatgttaaac
    11161 gaacaaagaa aacaagaagt aactgaaata tgccaaaaat taattcaaaa tcctagttct
    11221 tctggaaatg aagaaggtgt tgtaaaagca atagaagagt cttttgaaaa attaggtttt
    11281 gatagttggt caagagatag atatggaaat atagtaggat gtataaaagg taacaagcca
    11341 ggtaagaaaa tattatttga tggacatata gatacagttc cagttccaga tgcaagtaaa
    11401 tggagtgttc ctccttttga aggaaaaata gttgatggaa agatatatgg tagaggaact
    11461 tctgacatga aaggtcaata tactgcaatg atgtctgctg tagcgtattt tgctaaagac
    11521 actaatagag attttgctgg agaactatat gtagcaggtg ttgttcatga agaaatattt
    11581 gaaggtgttt cggctagaga gataagtaag gctgttcaac ctgactatgt tgtaataggt
    11641 gagtcttcag agttaaactt aaagattggt cagagaggta gaggagaaat agtagtagaa
    11701 acttttggta aaccagctca ctctgctaat ccagaaaaag gt
//
LOCUS       DAELLV010000114        11681 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.114, whole genome shotgun sequence.
ACCESSION   DAELLV010000114 DAELLV010000000
VERSION     DAELLV010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11681
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.114"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(77..979)
                     /locus_tag="KPD58_002301"
     CDS             complement(77..979)
                     /locus_tag="KPD58_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG0266063.1"
                     /translation="MDIKQLKYFVEIVKSGFNLSIASKTLHISQPALSQIIKNFEESE
                     DVYLFERYKGRLNGLTPAGERFYVNAEHIINEYKNMMEDLREDSVQFKGKVRIGIPPL
                     ILGIAFSDVVAQLVANNPDIEFDIVEKGAYDLNRMLILQELDYAVLLHPHKIDKSVVT
                     EHVLQEDELTAFLNINHPLAKKDKIDWNDLNEQLLAIFNPTFMIHHKLMQKFTDENVK
                     LKRYIMSGSWDFLLLSTTNSDFITILPSPVHDFFENSKIIEKPFNHPITWKVILCRPK
                     KERYSHVDQHVFKFIIDFFSKKNS"
     gene            1334..2512
                     /locus_tag="KPD58_002302"
     CDS             1334..2512
                     /locus_tag="KPD58_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBG0266064.1"
                     /translation="MKDVVIVSAVRTPIGSFGGVFKNTSAVQLGTIAVKEAISRVRLN
                     LSEIDEVIIGNVLQTGLGQNVARQIAINAGIPNSVPSYTVNKLCGSGLKSVQLAAQSI
                     TSGENDVVIAGGTENMSQAPYIVPTARFGSKMGDITMVDSMLTDGLIDAFNQYHMGIT
                     AENIATKFEFTREMQDKLALESQNKAENAIKNNRFKEEIVPVDILIRRGKIETIDKDE
                     YPKLGMTFEGLAKLKPAFKKDGTVTAGNASGINDGAAMLILMSQQKADELGIRPLAKI
                     KSYASAGVEPEVMGTGPIPATRKALKKAGLSINDIDLIEANEAFAAQALAVKNELQID
                     SSKLNVNGGAIALGHPIGASGARILVTLIYEMQKRKVETGLATLCIGGGQGISMVVSR
                     "
     gene            2521..3177
                     /locus_tag="KPD58_002303"
     CDS             2521..3177
                     /locus_tag="KPD58_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HBG0266065.1"
                     /translation="MNKIVSIDEALSHVKDGDTIMVGGFMANGSPENLIDYLCSKNIK
                     DLTLICNDTGFIDKGVGKMVVNKQFKKIIASHVGLNKETGRQMNNNETDVELVPQGTL
                     AEQIRAAGYGLGGILTPTGIGTLVEEGKQKIVVDSKEYLLEKPIYADVALLFASKVDK
                     AGNLVYKGSMNNFNNLMASAAKITIVEADEIVEIGDIDPNEVNTPGIFVNYIVEGGNL
                     "
     gene            3177..3842
                     /locus_tag="KPD58_002304"
     CDS             3177..3842
                     /locus_tag="KPD58_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HBG0266066.1"
                     /translation="MDKLEMQEYIANRVSKELKDGAVVNLGIGLPTKVANYIPDNVNV
                     ILQSENGFLGLGSAEDGNSSDETIVNAGGQPVTILPGGCFFDSATSFGIIRGGHVDIT
                     VLGALQVDKYGNIANYMIPGKMVPGMGGAMDLVTGAKKVIVAMEHTSKGSAKILNNCT
                     LPLTATNAVDLIVTEMGVMEVTSDGILLKKINPAFTLDDVISATEAPLILSDSLNGKI
                     SVV"
     gene            3947..4723
                     /locus_tag="KPD58_002305"
     CDS             3947..4723
                     /locus_tag="KPD58_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HBG0266067.1"
                     /translation="MVKDKVVFVTGAASGIGKQIAESFLKNGAKVMFSDINQESLNNT
                     ITELQKKGYDCMSVKCDVTKEEEINHAIDKTVEKYGRLDILINNAGLQHVSMIEDFPT
                     DKFEFMIKIMLTAPFIATKKAFPIMKKQGFGRIINMASINGVIGFAGKAAYNSAKHGL
                     IGLTKVAALEAADSGITVNALCPGYVDTPLVRGQFNDLSKTRNIPLENVLEEVLYPLV
                     PQKRLLDVQEIADYAMFLASDNAKGITGQACLLDGGYTAQ"
     gene            4785..6119
                     /locus_tag="KPD58_002306"
     CDS             4785..6119
                     /locus_tag="KPD58_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBG0266068.1"
                     /translation="MSIIAPIGIIIGIIAIIYFSVKEIHITIAAPIATLIVVILNKMD
                     VVTSMLGADKNNYMGALGNYIMSYFAIFLLGSILAKFMEESGATVSIADFILNKFGSN
                     HPYRVLVAIFIVSFILTYGGISLFVVMFAVIPLARTLFKKLDISWNLIQIPLWLGIAT
                     ITMTIIPGTPAIQNVIPIQYLNTSLTAATIPSIVGSIGCATFGLVYMKYALNKSIRKG
                     ETYATYTSELEEESLDRELPGFFASIFPLLALVIIAITGSTFGSEFMKKNVIYIALIV
                     AILLSAFLFHKFLSSKIQTLSIGASGSIGPIFATSSAVAFGAVIMAAPGFDVFSKLIM
                     SIPGSPLISLTVLTSTMSAITGSSSGALGIVMPNFADYYLSTGLNPELIHRVATIASN
                     ILTIVPQSGVFLTFLSLSKLTHKTGFKETFIVVAVGSLIAEIIVITLGLLMY"
     gene            6616..7542
                     /locus_tag="KPD58_002307"
     CDS             6616..7542
                     /locus_tag="KPD58_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HBG0266069.1"
                     /translation="MMRRNIPTKLKTILALGVLTLATASSAVTASASSLENNVNISNK
                     SLVYSNTKYKFSAKPNCSVKPELKPDKDNSCKDKNHNDKNCNNTNKPEDNNNSDSTNK
                     PDNNKPESNKPEDNNNSGSTNKPDNNKPESNKPEDNNNSGSTDKPDNNKPESNKPEDN
                     NNSDSTNKPDDNNNSGSTSESFSAYQKEVVDLVNIERAKAGLNPLTLDSSISNVATKK
                     SQDMIDNNYFSHNSPTYGSPFDMLKKFGISYKTAGENIAMGQKTPKEVVNAWMNSEGH
                     RKNIMNPNFSKIGVGVAQKSGGSIYWTQIFVG"
     gene            complement(7935..11474)
                     /gene="nifJ"
                     /locus_tag="KPD58_002308"
     CDS             complement(7935..11474)
                     /gene="nifJ"
                     /locus_tag="KPD58_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBG0266070.1"
                     /translation="MAKFMKTLDGNTAAAHVAYAFTDVAAIYPITPSSTMAEVVDEWA
                     SQGRKNIFGQTVNVVEMQSEAGASGTFHGSLQGGALTSTFTASQGLLLMIPNMYKVAG
                     ELLPGVFHVSARALASQALSIFGDHQDVMAARQTGCVLLASGSVQEVADIAPVAHLAA
                     IEGRLPFIHFFDGFRTSHEIQKVELLENEDYASLLNFEAVQAFRDNALSPNHPVARGT
                     AQNPDIYFQTREASNKYYQNIVGIVEKYMEKMSNLTGRKHSLFDYYGAEDAKYVLIAM
                     GSVTETIEETIDYLNAKGEKYGLVKVHLYRPFSMKHFLDAMPSTVERICVLDRTKEPG
                     STGEPLYLDVRDVFYGKENAPMIIGGRYGLGSKDTTPSDIKTVFDNLVSEQPKNGFTV
                     GIVDDVTNTSLTPSEPIKIASKGTIRCKFWGLGSDGTVGANKQAIKIIGDHTEKYAQA
                     YFDYDSKKSGGITMSHLRFGDTPIRATYLIDEADYIACHKQSYVYQYDLLKGLKKGGT
                     FVLNTIWDQAGLEEHLPAHMKQYIAKNDIKFYTVNAVKLGQEIGLGNRINMIMQSAFF
                     KLAEIIPEEDAVKYLKDSIVKAYGKKGEKIVNMNYAAVDAGINALVKVEVPASWENAV
                     DADKGEVKEPEFIKNILRPMTAQEGNNLPVSTFNGIEDGTFPCGTAAYEKRGIAVDVP
                     EWIIDNCIQCNQCSFICPHACIRPVLVTEEELANAPEGFEAKKALGKGLEGLKYRIQV
                     SPLDCTGCGNCADICPAKEKALVMKPIDTQLDTELDNWAFAVNPDEVAPKVDVMPANT
                     VKGSQFRQPLMEFSGACAGCGETAYIKVVTQLYGDRMMIANATGCSSIWGASAPSIPY
                     TCNHEGKGPSWANSLFEDNAEYGFGMYTAVKQIRNKIVDAMTELVSMDICEDAKAVFT
                     EWLDSRNDGEASKVASAKVVELLEKPACDCTDEKAKELVKAIKDRKDYLVKRSQWILG
                     GDGWAYDIGYGGLDHVLASGEDVNVLVFDTEVYSNTGGQASKATPAAAMAKFAASGKK
                     SRKKDLGMMAMTYGNVYVAQVAMGADKNQFIKAVLEAEKHDGPSLIIAYAPCINHGLK
                     EGMGRTVANEAQAVACGYWHLYRFNPEVKAEGKNPFTLDSKEPTASFKDFILAQVRYS
                     AIAKQFPEEADGLFAKAEADAKERYEGYKKLAE"
BASE COUNT         4043 a         1877 c         1776 g         3985 t
ORIGIN      
        1 tcaaacatat agatgaaaaa ctaatagttt attataataa aaattgattt atttatttgt
       61 tattttaata acatttttaa gagttctttt tactaaagaa atctataata aatttaaata
      121 catgttggtc tacatggcta taacgttctt ttttaggtct acatagtata actttccatg
      181 taataggatg attaaaaggc ttttctataa ttttgctatt ttcaaaaaaa tcatgaactg
      241 gtgatggtag aattgttata aaatcagaat ttgtggttga tagcaacaaa aaatcccatg
      301 aaccagacat tatatatctt tttaacttaa cgttttcatc agtaaatttt tgcattagct
      361 tatgatgtat cataaaagta ggattaaata ttgcaagtaa ctgctcattc aaatcgttcc
      421 aatctatctt atccttctta gcaagtggat gatttatatt taaaaatgct gtaagttcat
      481 cttcttgaag cacatgttca gttactacac ttttatcaat tttatgtgga tgtaacaata
      541 ctgcataatc taattcctgt aagataagca ttctatttaa gtcatatgca cccttctcta
      601 ctatatcaaa ttctatatct ggattatttg caactaactg tgcaactaca tctgaaaatg
      661 ctattccaag tataagtgga ggtattccta ttctaacttt tcctttaaat tgaactgaat
      721 cttctcttaa atcttccatc atatttttgt attcatttat gatatgttct gcatttacat
      781 aaaatctttc tcctgctggt gttaacccat ttaacctgcc tttatacctt tcaaataaat
      841 atacatcttc actttcttca aaatttttta taatttgact taatgctggt tgagatatgt
      901 gaagtgtctt agatgctatt gataaattaa aaccagattt cactatttca acaaaatatt
      961 tcaactgttt tatatccatt ttaaaccccc ttccccaaaa gtataattta gattatattt
     1021 tttcttctaa tgatatttta taggaaaatc aacataattt cattaatatt ttaacaaaat
     1081 atttttctaa atctactata aatttttatc ctataaattt ttcttataca ctatataaaa
     1141 aattagactt agacaaaacg cttttttcgc cgtaatctat aattagaaac aggaaaacct
     1201 aaagcattag gaataaaagt aattgaaata cttaaaaaca agcttgtatt aaaaaagtac
     1261 tttattatag tgttccctaa tttctttatt cagaaaattt ttaattatca ttcaagaaag
     1321 gtaactatta gatatgaaag atgtagtaat cgtatcagca gtcagaacac ctataggaag
     1381 ttttggaggt gtatttaaaa atacttcagc tgttcaactt ggaactatcg ctgttaaaga
     1441 agcgatatca cgtgtaagac ttaatttatc tgaaatagat gaagtaataa ttggaaatgt
     1501 tcttcaaaca gggcttggac agaatgttgc tagacaaatt gctataaatg caggtatacc
     1561 aaattctgtt ccaagctaca cagtaaataa attatgtggt tcagggctta aaagtgtcca
     1621 gctagctgcc caatcaatca catctggtga aaatgatgtg gtaattgcag gtggtactga
     1681 aaatatgagc caagctcctt atatcgttcc aactgctcgc tttggtagta aaatgggtga
     1741 cataaccatg gtagatagta tgctaacaga tggccttatt gatgctttca atcagtatca
     1801 tatgggaata actgcagaaa atattgcaac taaatttgaa tttactagag aaatgcaaga
     1861 taaattggca ttagaaagtc aaaataaggc tgagaatgct ataaaaaaca accgctttaa
     1921 agaagaaatc gttccagtag atattttaat acgtcgagga aaaatagaaa ctattgataa
     1981 agatgaatat cctaaattag gtatgacatt tgaaggttta gctaaactta aacctgcatt
     2041 caaaaaagat ggtactgtaa cagctggaaa tgcctcaggt attaatgatg gtgcggctat
     2101 gttaatacta atgagccaac aaaaagcaga cgaactaggt attagaccac ttgctaaaat
     2161 aaaatcctat gcttcagctg gtgttgaacc tgaagtcatg ggtactggtc caatccctgc
     2221 aactagaaaa gcactgaaaa aagctggact tagcatcaat gatatagact taatagaagc
     2281 aaacgaagcg tttgctgccc aagcattagc tgttaaaaat gaacttcaaa tagattcatc
     2341 aaagttaaat gttaatggtg gtgcaatagc attaggacat ccaataggag caagtggagc
     2401 tagaatactt gttaccttaa tttatgaaat gcaaaaacgt aaagttgaaa ctggacttgc
     2461 aacactttgt attggtggtg gtcaaggaat atcaatggtg gtttcacgat aggaggaaat
     2521 atgaataaaa tagttagcat tgatgaagct ctatctcatg taaaagatgg agatacaata
     2581 atggttggtg gattcatggc caatggttca ccagaaaatt taattgatta tttatgtagt
     2641 aaaaatataa aagatttgac actaatttgc aatgatactg gctttattga caaaggagtt
     2701 ggcaaaatgg ttgtaaataa gcaatttaaa aagataattg cttctcatgt aggattaaat
     2761 aaagaaactg gaagacaaat gaacaacaat gaaacagatg ttgagttagt tcctcaggga
     2821 actcttgctg aacaaatacg tgcagctggt tatggtctag gaggtatact cacaccgact
     2881 ggtattggta ctcttgttga ggagggaaag caaaaaattg ttgtcgatag caaagaatac
     2941 cttcttgaaa aaccaattta tgcagatgtt gctcttctat ttgcatcaaa ggtcgataaa
     3001 gcaggtaatc tagtttataa aggctctatg aacaatttta ataatttaat ggcatctgct
     3061 gccaaaatta caatagttga agcagatgaa atcgttgaaa ttggtgatat tgacccaaat
     3121 gaagttaata caccaggtat ttttgtgaat tacatcgttg aaggaggtaa tttataatgg
     3181 ataaattaga aatgcaagaa tatattgcaa atagagtatc taaagaatta aaagacggag
     3241 ccgttgtaaa ccttggaata ggtctaccaa ccaaagtagc aaattatatt ccggataatg
     3301 taaacgttat cttacaatct gaaaatggat ttttaggttt aggttcagcc gaagatggaa
     3361 attcaagtga tgaaactatt gtaaatgcag gtggtcaacc agttactata cttcctggag
     3421 gatgtttctt tgatagtgct acttcttttg gaataatcag aggaggtcat gttgatataa
     3481 ctgttcttgg cgcacttcag gttgacaaat atggcaatat agcaaactat atgattccag
     3541 gtaagatggt tccaggtatg ggtggagcta tggacctagt aactggagct aaaaaagtta
     3601 ttgtagcaat ggaacatact tcaaaaggtt ctgcaaaaat attaaataat tgtactcttc
     3661 cacttactgc aacaaatgct gtagatttga tagttactga aatgggagtt atggaagtaa
     3721 catcagatgg aattttactt aaaaaaataa atccagcttt cacattagat gatgttattt
     3781 ctgctactga agctccttta atattgtcag attcattaaa tggaaaaata tctgttgttt
     3841 aaaataatta aaactagaaa ataaaaatct cttagacgta ttacatataa atttgtattg
     3901 cttaaaatat taatataaaa ataaaataaa attggaggaa atgaaaatgg ttaaagataa
     3961 agttgtattc gtaacaggag ctgcaagtgg aataggtaaa caaatagctg aatcgttctt
     4021 aaaaaatggt gctaaagtaa tgttctctga tataaatcaa gaatctttaa ataataccat
     4081 aacagagtta caaaaaaaag gttatgattg catgagtgta aagtgtgatg taactaagga
     4141 agaagaaatt aatcacgcta tagacaaaac tgttgaaaag tatggcagat tagatatatt
     4201 aataaacaat gctggacttc aacatgtttc tatgatagaa gatttcccaa ctgataaatt
     4261 tgaatttatg ataaaaataa tgctgacagc accttttata gctactaaaa aagcatttcc
     4321 tataatgaaa aaacaaggct ttggtcgtat aataaatatg gcctcaatca atggtgtaat
     4381 aggatttgct ggaaaggctg cttataactc agcaaaacat ggacttattg gactaacaaa
     4441 agttgctgct cttgaagctg ccgattctgg tataacagtt aatgctttat gccctggata
     4501 tgtagatact ccacttgtca gaggtcagtt caatgacctt tcaaaaactc gtaatatacc
     4561 attggaaaat gtcttagagg aagttttata cccacttgta ccacaaaaac gcctattaga
     4621 tgttcaagaa attgccgact atgctatgtt cttagcaagt gataatgcta agggtattac
     4681 aggacaagct tgcttactag atggaggata tactgcacaa tagttattat atttagggct
     4741 tgaagaaagt attcttcagg tccttctact aaggagggat atttatgagt attatcgcac
     4801 caattggtat aattattggt atcatagcta ttatatattt ttcagttaaa gaaattcata
     4861 taactatagc tgctccaata gcaactttaa tagttgttat tttaaataaa atggatgttg
     4921 taacgtcaat gctaggtgct gacaaaaaca attatatggg tgcacttgga aactatataa
     4981 tgagttattt tgcaatattc ttattaggct caatacttgc taaatttatg gaagaaagtg
     5041 gagctactgt atcaatagct gattttattt taaataaatt tggctctaat catccttata
     5101 gagttttagt tgctatattt atagtaagtt ttatattaac gtatggtggt ataagcctat
     5161 ttgtagttat gtttgcggtc attccacttg caagaactct atttaaaaaa cttgatattt
     5221 cttggaacct tattcagata ccactttggc ttggtattgc aacaattact atgactataa
     5281 ttcctggaac accagccatt caaaatgtta tacctattca atatctaaat acgtctctta
     5341 ctgcagctac tataccaagt attgtaggta gtataggttg tgctacattt ggacttgtct
     5401 atatgaaata tgctctaaac aaaagtatta gaaaaggtga aacttatgca acttacactt
     5461 cagaattaga agaagaatct ttagatagag aattaccagg attttttgca agtatttttc
     5521 ctctattagc actcgtaatt atagctataa ctggaagtac ttttggttca gaatttatga
     5581 aaaaaaatgt aatttacata gctttaatag ttgcaattct tttatctgct tttttattcc
     5641 ataaatttct atcatctaag atacagacat tgagcatagg tgctagtggt tctataggtc
     5701 ctatatttgc tacatcttct gcagttgcat ttggagcagt aataatggct gcacctggtt
     5761 ttgatgtatt tagcaaactt ataatgtcaa taccaggtag tccacttata agtcttactg
     5821 ttttgacttc aacaatgtca gcaataacag ggtcctcttc tggtgcatta ggaatagtaa
     5881 tgccaaactt tgcagattat tatttaagta ctggtctaaa tccagaatta attcaccgtg
     5941 ttgctacaat agcctcaaat atactaacta tagttccaca aagtggtgtt tttcttacat
     6001 tcttaagctt atctaagtta acacataaaa ctggatttaa agaaactttc attgtagttg
     6061 cagttggttc actaatagca gaaattattg taattacatt aggattacta atgtattaat
     6121 ataaaacaaa ttaaaattca taatatgaaa ggtggtgtct caaagtgaac tttttactta
     6181 atgagacact ctttttatat gaaataacat aagcctatca tttcaacaat aaaaaagaac
     6241 cttatctcta ttttgagaca gacccttttt atttcttaaa gatttaaata attataattt
     6301 gttgctaata cgaaagcatc tactgaataa atttaaccaa ataaaaaaaa atttatcata
     6361 actataaatt tacgttatgg aattattata aaaatattat tttctctaac taagtaattc
     6421 catactatac aggcttttaa tacaaattgt caaacacact gaaatatacc tatttattcc
     6481 atatatatct tttttacata tctttacagt aaaaaaagtc ttaatcttat gttagtatag
     6541 attcacgggt aaccccctac actaaaggtt acaaatcagt aacattttta gaatttttag
     6601 attaagaggt gtttaatgat gagaagaaat ataccaacaa aattaaaaac tatattagct
     6661 ttaggagtgt taactttagc aacagcttca agtgctgtta ctgcaagtgc ttcatcactt
     6721 gaaaataatg taaatataag taacaaaagt ttagtatata gtaatactaa atacaagttt
     6781 tctgcaaaac caaattgttc tgtaaaacca gaacttaagc ctgataaaga taactcttgt
     6841 aaggataaga atcataatga caagaattgc aacaacacta ataaacctga ggacaacaat
     6901 aattcagact ctactaataa gcctgataac aataaacctg agtctaataa acctgaagac
     6961 aacaataatt caggctctac taataaacct gataataata aacctgagtc taataaacct
     7021 gaagacaaca ataattcagg ctctactgat aaacctgata acaataaacc tgagtctaat
     7081 aaacctgaag acaacaataa ttcagactct actaacaaac ctgatgataa taacaactca
     7141 ggctctacaa gtgaaagttt ttcagcttac caaaaagaag ttgttgactt agtaaatata
     7201 gaaagagcta aagctggatt aaatcctctt actcttgatt cttctatatc aaatgttgct
     7261 acaaaaaaat ctcaagatat gattgataac aactattttt cacacaattc tccaacttat
     7321 ggttctccat ttgacatgtt gaaaaaattt ggaataagct ataagacagc aggtgaaaat
     7381 atagctatgg gtcaaaaaac tcctaaagaa gttgtaaacg catggatgaa ttctgaaggt
     7441 cacagaaaaa atataatgaa tccaaatttc agtaaaatag gtgttggagt agcacaaaaa
     7501 agtggtggtt caatttattg gacacaaatc tttgtaggat aaaatactct ttttccatac
     7561 atatcaaaag agtatctcta agtgataaaa aacacaaatg agatactctt ttgtatctat
     7621 tttaaaatta tcaaatccaa atatacatac tataaatatt atttgttctc ttaattttag
     7681 tgtgtagagc aatatataaa aagtattggc aagatgaaaa tggaaaactt aaaaaagata
     7741 tttaataggt aaaaacaaaa tcttaatatc ttcataaaaa aaggctgtct caaaatagag
     7801 ataaggcctt tttcattgtt gaaatgatta aaatatattt gatttcatac aaaaagagtg
     7861 cctcatgaac taaaaagctc actttgagac actctctttt atcaattcta tataagcatt
     7921 ataatacttt aatattattc agctaatttt ttgtatcctt catatctttc tttagcatca
     7981 gcttcagctt tagcaaataa tccatcagct tcctctggga attgtttagc tatagcagaa
     8041 tatcttactt gtgcaagtat gaaatctttg aagcttgcag ttggttcttt tgaatctaaa
     8101 gtgaatgggt ttttaccttc agcttttact tctggattga atctgtataa atgccagtat
     8161 ccgcaagcaa cagcttgtgc ttcattagct acagttcttc ccataccttc ttttaatcca
     8221 tggtttatac atggagcata agctattatt agagatggcc catcatgttt ttcagcttct
     8281 aaaacggctt tgataaattg atttttatca gcacccatag caacttgagc tacatatacg
     8341 tttccatatg tcatagccat cattcctaaa tctttctttc tagatttttt acctgaagca
     8401 gcaaacttag ccatagcagc agctggagta gctttagaag cttgacctcc tgtatttgaa
     8461 tatacttctg tatcaaatac aagtacgttt acatcttcac cagatgcaag aacatggtct
     8521 aatccaccat aaccgatatc atatgcccaa ccgtctccac ctagtatcca ttgagatctc
     8581 ttaactagat aatcttttct atcttttata gcttttacta attctttagc tttttcatct
     8641 gtacaatcac aagctggttt ttctaataat tcaacaacct tagcacttgc tactttagaa
     8701 gcttctccat catttctaga atctaaccac tctgtaaata ctgctttagc atcttcacag
     8761 atatccatag aaactaactc tgtcatagca tctactattt tatttcttat ttgcttaaca
     8821 gctgtgtaca taccaaatcc atattcagca ttatcctcga ataaagaatt tgcccaagat
     8881 ggacctttac cttcatggtt acaagtataa ggaattgatg gtgcagaagc tccccatata
     8941 gaagaacatc cagtagcatt agctatcatc attctatctc catataattg tgtaactact
     9001 ttaatgtacg cagtttctcc acatccagca catgctcctg aaaactccat taatggttgt
     9061 ctaaattgac ttcctttaac agtatttgca ggcataacat caacttttgg agctacttca
     9121 tcaggattta ctgcaaatgc ccaattatct aactctgtat caagttgagt atctattggc
     9181 ttcataacta aagctttttc tttagctgga catatgtcag cacagtttcc acatcctgta
     9241 cagtcaagtg gactaacttg aattctatat tttaatcctt ctaatccttt acctaatgct
     9301 tttttagctt caaatccttc tggagcatta gctaattctt cttcagtaac taatactggt
     9361 cttatacatg catgaggaca tataaaagaa cattggttac attgaataca gttatctatt
     9421 atccattcag gaacatcaac agctatacca cgtttttcat aagctgcagt accacaaggg
     9481 aatgttccat cttctatacc attgaatgta cttactggta agttgtttcc ttcttgtgca
     9541 gtcattggtc ttagtatatt ttttataaat tctggttctt taacttctcc tttatctgca
     9601 tcaacagcat tttcccaaga agctggaact tcaactttaa ctaaagcatt tattcctgca
     9661 tcaacagcag catagttcat attaactatt ttttcacctt ttttaccata agctttaact
     9721 atagaatctt ttaagtattt aacagcatct tcttctggta ttatttcagc taatttaaag
     9781 aatgcagatt gcattatcat gtttattctg tttccaagac ctatttcttg tcctagttta
     9841 acagcgttaa ctgtatagaa ttttatatca tttttagcta tatattgttt catatgagct
     9901 ggtaaatgtt cttcaagtcc tgcttggtcc catatagtgt taagtacaaa tgtaccacct
     9961 tttttaagac cttttaataa atcatattga taaacatatg attgtttatg acaggcaata
    10021 taatcagctt catctattaa ataagtagct cttattggag tatcaccaaa tcttaagtga
    10081 gacatagtta taccaccaga ttttttagag tcataatcaa agtaagcttg agcatatttt
    10141 tcagtatggt caccgattat tttgatagct tgtttattag ctccaacagt accgtcagaa
    10201 cctaatcccc agaacttaca tcttatagtt cctttagaag ctatttttat tggctcagat
    10261 ggagttaaag aagtattagt tacatcatca actataccta ctgtaaatcc atttttaggt
    10321 tgttctgaaa ctaagttatc aaaaacagtt tttatatctg aaggagtagt atcttttgaa
    10381 cctaatccgt atcttccacc tattatcata ggagcatttt cttttccata gaatacatca
    10441 cgtacatcta agtataatgg ttcaccagtt gaacctggct ctttagttct atcaagtaca
    10501 catattcttt caacagtaga tggcatagca tctaagaaat gtttcattga gaatggtcta
    10561 tataaatgaa ctttaactaa accatatttt tctcccttag catttaagta atctatagtt
    10621 tcttctattg tttctgttac tgaacccata gctattaata cgtatttagc atcttctgct
    10681 ccatagtaat caaataaact atgttttcta cctgttaaat tactcatttt ttccatgtat
    10741 ttttcaacta tacctactat attttgatag tatttattag aagcttctct agtttggaaa
    10801 tatatatctg ggttttgagc agtaccacga gcaactggat gatttggaga taaagcatta
    10861 tctctaaatg cttgaactgc ttcaaagttt aataaacttg cataatcttc attttctagt
    10921 aattcaactt tttggatttc atgtgaagtt ctgaatccat caaagaaatg tataaatggt
    10981 aatctacctt ctattgcagc taaatgtgca acaggtgcta tatcagctac ttcttgaact
    11041 gaaccagaag ctaataatac acatccagtt tgtctagcag ccataacgtc ttggtgatcc
    11101 ccaaatattg ataacgcttg agatgctaaa gcacgagcac ttacatggaa tactcctggt
    11161 aataactctc ctgcaacttt atacatgtta ggtatcatta ataataaacc ttgagatgct
    11221 gtaaaagtag aagttaaagc tcctccttgt aaagaaccgt ggaatgttcc tgatgcacct
    11281 gcttctgact gcatttcaac aacattaact gtttgtccaa atatattttt tcttccttgt
    11341 gatgcccact catcaactac ttcagccata gttgaagatg gtgtgattgg atatatagct
    11401 gctacatctg taaacgcata ggcaacatga gctgcagctg tatttccatc aagtgttttc
    11461 ataaacttag ccataatatg tatcctcctt gatagctaca attgtacttg tttataacaa
    11521 gtctattttt ttatatttac atataattta cacaacaaaa atataaacac acatatagtc
    11581 gcgcaaatac tctatcttct aatataatac tacagattag ataaaattcc tttgaaaatt
    11641 gtaaattaat tacatattta agtagatttt ttttaaaatt a
//
LOCUS       DAELLV010000115        11641 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.115, whole genome shotgun sequence.
ACCESSION   DAELLV010000115 DAELLV010000000
VERSION     DAELLV010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11641
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.115"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(1132..2097)
                     /locus_tag="KPD58_002309"
     CDS             complement(1132..2097)
                     /locus_tag="KPD58_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266071.1"
                     /translation="MVMQVQEKILELLYTINEDVVSIKNDMSEVKQEINVLKEDMSEV
                     KQEITVMKEDMSEVKQEINVLKGNMSEVKQEMIVMKEDMSEVRQEINIMKEDMSEVRQ
                     EINTMKEDMSEVRQEMTVMKEDMSEVKQEINVLKEDMSEVRQEINIMKEDMSEVRQEM
                     TVMKEDTSEVKQEINVMKKDMSEVRQEITVMKEDVSEVKSEVNFMQNKINNINEDMNG
                     IKDEVSIANEKLYGIEIKIDSLECEDKSMKEIQIEQNNILGSLLHNSEINKATHDNIE
                     HNIAYIKGDTNSIKEDIAEIRRDLNLVELATSKNWSDIVKLKSVK"
     gene            complement(2327..3412)
                     /locus_tag="KPD58_002310"
     CDS             complement(2327..3412)
                     /locus_tag="KPD58_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBG0266072.1"
                     /translation="MYNFLGKELKSPLIIGSGPLTYSAAGCKILSDAGAGAVVTKTIR
                     KERAINPAPHMVRNTANALLNNEKWTDFEPEQWIDFEIPQMKRDGTVCIASIGHTIEE
                     SSELVEKVANAGADFIELVSYDYRDLIPMLKDAKERVNIPVIVKLPPMIDEIGDFAKK
                     LEEAGADAITACDSVGPAFRIDIETGQPLLGGNGIGYLSGETIKPITLQRIYEIKKQV
                     NIPIIGLGGCVSGDDALEMIMAGADFVGICSVVILKGAQVISKIHDDLKSNLNRLGYN
                     TIENACGIVHNHMGDVNERLSFEFTYDEEKCTKCKMCERVCAYQARKLNDKMELNEEE
                     CRYCGLCISVCKPKALGRKISCSELNV"
     gene            complement(3448..4761)
                     /locus_tag="KPD58_002311"
     CDS             complement(3448..4761)
                     /locus_tag="KPD58_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG0266073.1"
                     /translation="MIAIKSKYLISSADEIYANSAVVVENDIIKDILPNEEVEIKYKN
                     IEEIIDKSDAIIMPGFVNGHMHQYGVLSRGIPANVHFTDFEGFLNDYWWPFIENRIGL
                     KEVKATTKASAIELIESGVVAFCDTLEAPNTEEGTLIEQGKILEEIGMKAILSLESCE
                     RISFENGLRCLDENSNLIKWSRENSKLINGIMCTHTSFTCSDRFIKKAKEDAKKLNSP
                     MQFHLCESIYEPNYAEKHFGKKAVDYYNDLDILDETILASQCVKVNDEEIDILKEKGV
                     KVVHMPLSNCEVGGGFSPVPKMIKKGIKVALGTDGYINDFFEVMRGAFLMHKSVEEDA
                     SVMPANLVFRMATEHGAYVLGLQNSGKIAVGNKADIIVMEDEFKTPVTLDNIFDQIVV
                     QGKKEFISNVYIDGRHILKEKQLVDLDKKAIVKEMKEVACEFWKF"
     gene            complement(4762..7053)
                     /locus_tag="KPD58_002312"
     CDS             complement(4762..7053)
                     /locus_tag="KPD58_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG0266074.1"
                     /translation="MVNKEVRKIDSEAIVTGKPIYTEDLIFHKDVLTIKLLRSPHAFA
                     KIKNIDIKNALKVKGVVNIFTYKDVPNYRYSMVGESYPEASPHDQLILEDVVRYVGDE
                     VAIIAAEDERAAIKAMKLIKVEYEVMTPILDARESEGNKILIHEEDDLFCPFDFGLDA
                     TKNIVSREKMGKGDVEKEFKESDVIVEHSYMTQSQAHCMMETHRAYTYIDTNGRLVVT
                     SANQSAYHMRRQVSKALGIPLSKVRVIKPRIGGGFGGKNIAVTEIYASFVTWMTKRPA
                     KLIYTREETFAMTNTRHQMFFDIKIGADKEGNIKAIDMKALNNTGAYGDNGPSVCSES
                     GHNVLPTYNNVPAIKFDGRTVYTNLVPGGALRGYGATQGTFALDCAIDELAHKLNMDP
                     TEIKLKNIIKKQTEGGIMNFPIRSCALDECIIRGKKLIGWDEKYPVRDIGNNKVRAVG
                     MSVGTHGSGIANIDMAIVNMRMDEDGTYKLFSGSSDLGTGSDTILAQIAAKALNTSID
                     RISVYTGDTDMCPYDTGAYASCTTYVTGNATIKSAESLKQKILKVAQKKLDLPIDDLI
                     LYEDRVCYSEDVEKFVLLSELGKESVGGGNQEVLYSSESYGIQESAKPFLAGFAEIEL
                     DKTTGEFKVINYACSTDCGTVMNPALARIQVEGGVTQGIGLAMYEDPRHGSDGKLQTS
                     NLLQYKVPTKKDIGNIIVDFVDNYEPTGPYGAKSLGEIVIHTPAPAIANAIYNATGVR
                     IRELPITSEKVYMAMKKLNQGGK"
     gene            complement(7043..7837)
                     /locus_tag="KPD58_002313"
     CDS             complement(7043..7837)
                     /locus_tag="KPD58_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBG0266075.1"
                     /translation="MITIKEYVVPKSLDEAYELLISRKNNIILGGCGFLKLGSKNIGS
                     AIDLKDLALDYINETDDSILIGADTSLRTLELNKVIKNYCNGVISNAVSNIVGVQFRS
                     GARVGASVFAKYGFSDLIPSLLVVDAKVKLYKKGVMNLADFLESELEKDILIEVILPK
                     KDAIGVFDSIRKCTGDFAVLNGAMLKEGSIYKIAIGARPRRAKIAYKASEILSLENDI
                     EKAGEVSSEELSFGSNIRGSKEYRKDMAKALVVRMYNSIGGECDGK"
     gene            complement(7876..8322)
                     /locus_tag="KPD58_002314"
     CDS             complement(7876..8322)
                     /locus_tag="KPD58_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HBG0266076.1"
                     /translation="MIIKCTINNKEKELSIRPDDFLADTLRENGYVSVKKGCDTGSCG
                     LCTVMVNDKAVLSCNYLTARVDGQHITTLEGVLDEAKKFGDFLADEGADQCGFCAPGF
                     IMMVLAMKRELQNPTEEEVIDYVNGNLCRCTGYKGQLRAIHKYLNT"
     gene            complement(8394..9800)
                     /locus_tag="KPD58_002315"
     CDS             complement(8394..9800)
                     /locus_tag="KPD58_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0266077.1"
                     /translation="MMDILIKNAIIVTVNKEREVIFDGALVVKDNKIADIGNSKEIES
                     KYTDVKKIIDAKGKVLFPGFINTHNHLFQTLLKGLGDDMVLKDWLETMTFPAANYLEP
                     KDTYDAAMLGCIEGLRSGITTMVDYMYPHSKPGLCDGIIDAYKELGIRGILGRGCMNT
                     GAQFGVHPGIMQDVETVEKDVRRLFEKHHNTENGRIKIGVAPAAIWSNSQEMLEMLWR
                     VVKEYDDALFTVHISETPFDREAAKELHGQYDIDVLEKLGILGPNVLMVHCVYLTEKD
                     MELTKKYDMKVSHNTASNMYLSSGVAPVPEMLKKGITVSLGVDGAASNNSQDMLELMK
                     LTALQHKVNKCDPLAMSAEKVLELATIDGARAIGMEDEIGSLEIGKKADLLIFNPMLS
                     PKAIPMHNPVSTLVYSSSMKNIESVIVDGNIIMEDSKILTANEEKALKDAQDTAERLC
                     VRGTIKNRMEGHKWNSLY"
     gene            complement(9818..11188)
                     /gene="hydA"
                     /locus_tag="KPD58_002316"
     CDS             complement(9818..11188)
                     /gene="hydA"
                     /locus_tag="KPD58_002316"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HBG0266078.1"
                     /translation="MGTILRGGTIITSDKTYISDLRIEDEKIVEIGNNLEINGDKVID
                     ATGKIVIPGGIDTHTHFDMNAGSITTADNFETGTRAAIAGGTTTILDFAEANEGEDLL
                     QGVEAYHKKASGNCYCDYGFHMTITCLNKDTFNHMEKLISDGIVSFKMYMAYDGMKVD
                     DGTIYRVLKKARDLGCIVGFHCENGDLLDVLIEENIQNGNLEPKYHPLTRPNIVEKES
                     VSRLADITKLSNSKSYVVHLSCRESLETVKMAREKNIDMIVETCPQYLLLEDNLYNKD
                     KFEGAKYVMSPPLRKKEDINYLWEGLSSGDIQTVGTDHCSFNFKGQKDLGIDDFSKIP
                     NGAPGVEHRLALLYTYGVLENRISVNKFVEVTSTNAAKIFGMYPKKGEIAVGSDADIV
                     ILNINKEETISYKTQKQNVDYTPYEGFKVKCKVEDVFLRGNHVVQSCNVKEHPTGQYI
                     KRKITK"
     gene            complement(11321..>11641)
                     /locus_tag="KPD58_002317"
     CDS             complement(11321..>11641)
                     /locus_tag="KPD58_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBG0266079.1"
                     /translation="VEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDS
                     EITQYSFCTNGSHYAGEAGIKTIGFGPSKENLAHTIDEYIEQEQLFIGTEGYYGILKS
                     VYGK"
BASE COUNT         3417 a         2310 c         1213 g         4701 t
ORIGIN      
        1 tattatagct atgaattttt aattttttat agtaaattta attcaattta aatctttcaa
       61 tcttaatcat tataagttat aagttttcat aagtatttac atgtttcaag ttttaaacat
      121 ttacctataa acgctacttt atagatgtca cttcaaatcc agtgacctca tactttcata
      181 tgagcgtaga ctatttcttc atcttcagca ttacctgcta agagcaaccc gtttccacta
      241 tcaatcgctt atagtgtact ctctctcgag atagtcgttt gaccttccta tttctaggct
      301 tggcgaccaa atgtccattt ttaagtactt aggctctcac catataccat cctcactatt
      361 gtttctggtt ttcaccacat tcataaaagt ttctttctat cttttattgt agttgcaagg
      421 ctttaggatt tactggtttt aaagttgtgt cctacataga tttctctatg tacggggcca
      481 aaagtacaat ttatttaaca tactttgctt taccccctgt aaataatatt atttactctt
      541 tcactgtttt tgttattaaa ttaattaaat atcaattaat ctaagcataa aataattatt
      601 taatttaaac tcaaaatatt tatgaatatt ttataatata attctcataa gacagaaaaa
      661 cttaataagc tatgtatctc agttgtacca atgtactctc ctttactata tttgtatctg
      721 caacataaaa tatacaagtt tctatctatt tatagatata tatatctata agcaaaatag
      781 taatattatt tttatagtaa aaattttatt catattacta tttataattt ttatagagca
      841 attttagtgc caatatttgc atacatttgt atagtataaa atttttatgt tctcattgat
      901 aaaattttac ttatttcaag cgttctatta gattttatca tctaaatttt aggttttatt
      961 tttataaatt aattgtattt acataaaaaa aaagacctaa aacaaatttt taacggtctt
     1021 ttctcaattt tttattttca tttctaaaat ttataataaa tagtaagaac actataaatt
     1081 cttttatata aaattcttag tttgtggatg ataaattagt gaattaaaat actactttac
     1141 tgattttagt ttgacaatat cactccaatt tttactagtg gctaactcta ctaaatttaa
     1201 gtctcttctt atttcagcta tatcctcttt tatagaatta gtgtcacctt ttatatatgc
     1261 tatgttatgt tctatattat catgagtagc tttatttatt tcagaattat gtaataaact
     1321 tcctaaaata ttattctgtt ctatctgtat ctctttcatt gatttatctt cacattctag
     1381 actatcaatc ttaatttcaa ttccatataa tttttcattt gctatggata cctcgtcctt
     1441 aattccattc atatcttcat taatgttatt tattttattt tgcataaagt ttacttctga
     1501 tttaacttcg gaaacatctt ctttcattac agttatttct tgtctcactt ctgacatgtc
     1561 ttttttcatt acatttatct cttgttttac ctctgatgta tcttctttca ttacggtcat
     1621 ttcttgtctt acttcggaca tatcttcttt cattatattt atctcttgtc ttacttcgga
     1681 catatcttct tttaatacat ttatctcctg ttttacttcc gacatatctt ctttcattac
     1741 agtcatttct tgtcttactt ccgacatatc ctctttcatt gtatttatct cttgtcttac
     1801 ctcggacata tcctctttca ttatatttat ctcttgtctt acctcggaca tatcttcttt
     1861 cattacaatc atttcctgtt ttacttctga catatttcct tttaatacat ttatttcctg
     1921 ttttacttcc gacatatctt ctttcattac agttatttct tgttttactt ccgacatatc
     1981 ttcttttaac acatttattt cttgttttac ttccgacata tcatttttta tgctaactac
     2041 atcttcattt atagtatata ataactctaa aatcttctct tgcacttgca tcaccatccc
     2101 tattaattat tataacattt tacacaaatg ctatacaatg ttaacatata tatcagaatt
     2161 attaaaattt taattaaaca aaattgattc actttattac ttcaaaacta attaatatct
     2221 aaaataacta caaataaaca aaaataaggt caacaacaaa aaaggcttat ctctttgaga
     2281 tagcctttct tatttttatt taaaactttg ttttttataa aataatctat acattcagct
     2341 ctgaacatga aatttttctt ccaagtgctt ttggtttaca aactgatata caaagtccac
     2401 aatatctaca ctcttcttca ttcaattcca tcttatcatt caattttctc gcttgatatg
     2461 cacaaactct ctcgcacatc ttacatttag tacatttttc ttcatcataa gtaaactcaa
     2521 agctaagtct ttcattaaca tctcccatat gattatgtac tataccacaa gcattttcta
     2581 tagtattata tcctaatcta ttaagattag attttaaatc atcatgtatt ttagatatta
     2641 cttgtgcgcc ttttaaaatt actactgaac atataccaac aaagtcagca cctgccataa
     2701 tcatttctaa ggcatcatct ccacttacac atccacctaa gccaattatt ggtatattaa
     2761 cttgtttttt tatttcatat attctctgta aagttattgg cttgatagtt tctccactta
     2821 aatatcctat tccatttcca cctaataaag gttgccctgt ttctatgtct attctaaatg
     2881 ctggtcctac agagtcacaa gcagttattg catctgctcc tgcttcttct aattttttag
     2941 caaaatcgcc tatctcatct atcataggag gaagtttaac tattacaggt atatttactc
     3001 tttcctttgc atctttaagc attggtatca aatctctata gtcataagat actaattcaa
     3061 taaagtcagc tcctgcattt gcaactttct ctactaattc gctactttct tcaattgtat
     3121 gacctattga agctatacag acagtcccat ctcttttcat ttgtggtatt tcaaaatcta
     3181 tccattgctc tggctcaaaa tctgtccatt tttcattatt taaaagagca ttagctgtat
     3241 ttctaaccat atgtggtgct ggatttatag ctctttcttt tcttatagtc ttagtcacaa
     3301 ctgcccctgc tcctgcatca cttaatatct tgcatccagc tgcactatat gttaatggtc
     3361 ctgaacctat tataagtggt gattttaatt ctttacctaa aaaattatac attatctgct
     3421 tccccttttc atatatattt atattcgtta aaatttccaa aattcacaag caacttcttt
     3481 catttcttta acaatagctt ttttatctaa atcaactaac tgtttttctt ttaaaatatg
     3541 tctaccatct atgtatacat ttgatataaa ttctttttta ccctgaacta ctatctggtc
     3601 aaatatatta tcaagagtca caggagtttt aaattcatct tccataacta ttatatcagc
     3661 cttattacca actgctattt taccagaatt ttgaagtcct aatacataag caccatgctc
     3721 tgttgccatt ctaaatacta aatttgctgg cattactgat gcatcttctt caactgattt
     3781 atgcattaaa aatgcacctc tcataacttc aaaaaaatca tttatatatc catcagtacc
     3841 cagtgccact tttatgcctt ttttaatcat ttttggtact ggagaaaaac ctccacctac
     3901 ttcacagttg ctaagaggca tatgaactac ttttacacct ttttctttta atatatctat
     3961 ttcttcatca tttactttta cacactgaga tgctaatata gtttcatcca atatatctaa
     4021 atcattatag tagtcaactg cttttttgcc aaaatgtttt tcagcatagt ttggctcata
     4081 tatactctca cataaatgaa attgcattgg tgaatttaat ttttttgcat cttctttagc
     4141 tttcttaata aatctatcag aacatgtgaa gcttgtatgt gtacacatta ttccatttat
     4201 taatttacta ttttctctac tccactttat taaattagaa ttttcatcta aacatctaag
     4261 tccattttca aagcttattc tttcacaact ttctaaggat aaaatcgctt tcatgcctat
     4321 ttcttctagt atttttcctt gctcaattaa agttccttct tctgtatttg gagcttctag
     4381 ggtatcacaa aaagctacca caccagattc tataagttct attgctgatg ctttagttgt
     4441 tgccttcact tctttcaatc caattctatt ttctataaat ggccaccaat agtcatttaa
     4501 aaaaccttca aagtcagtaa aatgtacatt tgccggtatt cctctagaaa gaactccata
     4561 ttgatgcata tgaccattga cgaatcctgg cattattata gcatctgatt tatctattat
     4621 ttcttcaata tttttatatt ttatttcaac ttcttcattt ggcaatatat cttttataat
     4681 gtcattttct actacaacag cagaatttgc atatatttca tcagcagaag atattaagta
     4741 tttactttta atagcaatca tttatttacc tccctgattt aattttttca tggccatata
     4801 aactttctca gaagttatag gaagttctct tatacgaact cctgtggcat tgtatattgc
     4861 atttgctatt gctggtgcag gtgtatgaat aactatctct cctaaagatt tagctccata
     4921 tggacctgtt ggctcataat tatctacaaa atcaacaatt atgtttccta tatctttttt
     4981 tgttggaact ttgtattgta atagattaga agtttgaagc tttccatcag aaccatgtct
     5041 agggtcttca tacattgcaa gccctatacc ttgagttaca ccaccttcaa cttgtattct
     5101 agcaagtgca ggattcataa ctgtaccaca atctgtagaa caagcataat ttattacttt
     5161 aaactcccct gttgttttat ctaactctat ttcagcaaat cctgctaaga atggtttagc
     5221 actctcttgt ataccataag attcagatga atataaaact tcttgatttc caccaccaac
     5281 agattctttt cctaattcac ttaacaaaac aaacttctct acatcttcac tataacaaac
     5341 tctgtcttca tatagtatta aatcatctat tggtaaatcg agtttcttct gtgctacttt
     5401 taatatcttt tgttttaaac tttcagcaga ttttattgta gcatttccag ttacataagt
     5461 agtacaagaa gcgtatgcac cagtatcata tggacacata tctgtatctc ctgtataaac
     5521 ggaaattcta tcaatagatg tattcaatgc ttttgcagct atttgtgcaa gtattgtgtc
     5581 agaaccagtt cctaagtctg aagacccaga aaacaactta taagttccat cttcatccat
     5641 cctcatattt actattgcca tgtctatgtt ggctatacca gaaccatgag ttcccacaga
     5701 cattccaaca gccctcacct tattgttgcc aatatcccta acagggtact tttcatccca
     5761 tccaataagt tttttacctc taattataca ttcatcaagt gcacaacttc ttattggaaa
     5821 attcattatt ccgccttcag tttgtttttt tataatattc tttaacttta tttctgttgg
     5881 gtccatatta agtttatgcg ctaattcatc aattgcacaa tcaagtgcaa atgtaccttg
     5941 tgttgctcca tatcctctta atgctcctcc tggaactaaa ttagtatata cagttctccc
     6001 atcaaatttt attgcaggta cattattata tgttggaagt acattatgac ctgattcaga
     6061 gcaaactgaa ggtccattat caccataagc gcctgtattg ttcaatgctt tcatatctat
     6121 agcttttatg tttccttctt tatctgcccc aatttttata tcaaaaaaca tctgatgtct
     6181 agtattagtc attgcaaaag tttcttctct agtataaatt agttttgctg gtctttttgt
     6241 catccaagtt acaaatgatg catatatctc tgttacagct atgttttttc caccaaaacc
     6301 tccacctatt cttggtttta ttactctcac ttttgaaaga ggtattccca gtgctttaga
     6361 aacctgtctt ctcatatgat aagctgattg gtttgcagaa gttactacta atctgccatt
     6421 cgtatctata tacgtataag ctctatgagt ttccatcata caatgggctt gtgattgagt
     6481 catataagaa tgttctacta ttacatcact ttctttaaac tctttttcta catccccttt
     6541 acccattttt tctctcgata caatattttt tgtagcatcc aatccaaaat caaatggaca
     6601 aaacaaatca tcttcttcat ggattaaaat tttatttcct tccgattctc ttgcatccaa
     6661 tataggagtc attacttcat attcaacttt tataagtttc atagctttaa tagcagccct
     6721 ttcatcttca gcggctatta tagcaacttc atctcctaca tatctaacta cgtcttcaag
     6781 tattaattgg tcatgaggtg atgcttctgg atatgactct ccaaccattg aatatctata
     6841 atttggaaca tctttgtaag tgaatatgtt tactacacct tttaccttta atgcattttt
     6901 tatatctatg ttttttatct tcgcaaaagc atgtggactt ctaagaagct taattgttaa
     6961 aacatcttta tgaaaaatta aatcttctgt atatattggt tttccagtaa caatggcttc
     7021 actatctatt tttcttacct ctttatttac catcacactc acctccaata ctattataca
     7081 ttctcactac taaggctttt gccatatctt ttctgtattc tttacttcct cttatattac
     7141 ttccaaatga aagttcttca cttgatactt caccagcttt ttctatatca ttttctaaac
     7201 ttaatatttc acttgcttta taggcaattt ttgctcttct tggtctagcg cctattgcaa
     7261 ttttataaat acttccttct tttaacattg ctccatttaa tactgcaaaa tcacctgtac
     7321 attttcttat agaatcaaat acacctatag catctttttt aggtaatata acttctatta
     7381 atatatcttt ttcaagctca ctttctaaaa aatcagctaa gttcataacc ccttttttat
     7441 ataacttaac ctttgcatct accactagta atgaaggtat taaatctgaa aatccatact
     7501 tagcaaatac gctagctcct actctagctc cacttctaaa ttgaactcct actatattag
     7561 aaacagcgtt tgaaattacc ccattacagt aatttttaat aaccttattt aattctaaag
     7621 ttcttaaact tgtgtctgct ccaattaata tgctatcatc agtttcattt atataatcta
     7681 aagctaagtc ttttaaatct attgctgatc caatattttt actacctagt tttaaaaatc
     7741 cacatcctcc aagtattatg ttgttttttc ttgatattag cagttcataa gcttcatcta
     7801 aactttttgg aactacatat tcctttatag ttatcatact ctctccccat atcaacttaa
     7861 ttttcactta aaagtctaag tgtttaaata tttatgtatt gctcttaatt gacctttata
     7921 tcctgtacat ctacaaaggt taccatttac ataatctata acttcttctt ctgttggatt
     7981 ttgaagctct cttttcattg ccaaaaccat cattataaat ccaggagcac aaaatccaca
     8041 ctggtcagcc ccttcgtctg caagaaaatc tccaaacttc tttgcttcat ccaagacacc
     8101 ctcaagtgtt gtaatatgct gaccgtctac tctagcagta agataattac atgaaagcac
     8161 ggccttgtca tttaccatca ctgtacataa cccacatgag cctgtatcac accctttttt
     8221 aacacttaca tatccatttt ctcttaatgt atctgctaaa aaatcatctg gtcttatact
     8281 taattctttt tctttattat ttatggtaca tttgataatc aaataaatca cctcaaaact
     8341 catattttta agctaaagtt ttactatact tttctatgaa taaattacat ttattaataa
     8401 aggctattcc atttatgacc ttccattcta ttttttatag ttcctcttac acaaagtctc
     8461 tcagctgtat cttgagcatc ttttaatgct ttctcttcat tagctgtcaa tatttttgaa
     8521 tcttccatta ttatatttcc atctactata acactctcta tatttttcat actagaagaa
     8581 tatactaatg tagatactgg attatgcata ggtatcgctt ttggtgaaag cattggatta
     8641 aatattagta agtctgcttt tttacctatc tccaatgaac ctatctcatc ttccattcca
     8701 atagctcttg ctccatctat agttgccaac tcaagaactt tttctgctga cattgctaat
     8761 gggtcacact tattaacttt atgttgaagt gctgttaatt tcataagttc aagcatatct
     8821 tgagagttat tactagcggc tccatctact cctaaactaa ctgttattcc ttttttaagc
     8881 atttctggca ctggtgcaac acctgaagat aagtacatat tacttgctgt attatgagac
     8941 actttcatat cgtatttttt agtaagttcc atatcttttt cagttagata cacacaatgt
     9001 accataagca catttggtcc aagtattcct aatttttcta aaacatctat gtcatattgc
     9061 ccatgcaatt cttttgctgc ttctctatca aaaggagtct ctgatatatg tactgtaaat
     9121 aaagcatcat catattcttt tactactctc catagcattt caagcatttc ttgagaattt
     9181 gaccatattg ctgcaggagc tactcctatt ttgattcttc cattttctgt attatggtgt
     9241 ttttcaaaca atcttcttac atctttttca accgtttcta catcttgcat tattccagga
     9301 tgaactccaa attgagctcc tgtattcata catcctcttc caagtattcc tcttattcct
     9361 aattctttat aagcatctat tattccatca caaagacctg gcttactatg aggatacatg
     9421 tagtcaacca ttgttgttat accacttcta agtccttcaa tacatccaag cattgcagca
     9481 tcataagtat ctttgggctc taaatagtta gctgctggaa atgtcatagt ttctaaccag
     9541 tctttaagta ccatatcatc tcccaatcct tttaataaag tttggaataa atgattatgt
     9601 gtatttataa aacctgggaa taaaacttta cctttagcat ctataatttt tttaacatca
     9661 gtatatttac tttctatctc tttagaattt ccaatatcag caatcttgtt atcttttact
     9721 actaatgccc catcaaaaat aacttctctc tctttattca cagttactat tatggcattc
     9781 ttaataagta tatccatcat tcttatttcc ccccgcatta tttagtaatt tttcttttaa
     9841 tatattgacc tgttggatgt tctttaacat tacaactttg tactacatga tttcctctca
     9901 aaaatacatc ttcaacttta catttaacct taaatccttc atatggagta taatctacat
     9961 tttgtttttg agttttataa ctaattgttt cttctttatt gatatttaaa atgactatat
    10021 ctgcatcact tcctacagct atctcacctt ttttaggata cattccaaat atttttgcag
    10081 cattcgtaga agtaacttcc acaaacttat ttacacttat tctattttct aagaccccat
    10141 atgtatataa aagtgctaac ctatgttcaa caccaggtgc tccatttgga atcttactaa
    10201 aatcatcaat tcccaagtct ttttgcccct taaaattaaa tgaacagtgg tctgtgccaa
    10261 ctgtttgaat atctccacta gacaatcctt cccaaaggta atttatgtct tctttttttc
    10321 ttaaaggagg agacattaca tacttagcac cctcaaattt atccttatta tataaattgt
    10381 cttctaataa tagatactgt gggcatgttt ctactatcat atctatattt ttttctctag
    10441 ccatttttac agtttcaagt gactccctac aacttaagtg gactacatag gattttgaat
    10501 tagataattt agttatatca gcaagtcttg aaacagactc tttctcaact atgttaggtc
    10561 ttgtaagtgg atgatatttt ggctctaaat taccattttg tatattttct tctatcaata
    10621 catctagtaa atccccattt tcacaatgaa atccaactat acaacctaaa tcccttgctt
    10681 tctttaaaac cctgtatatt gttccatcat ctactttcat gccatcatat gccatataca
    10741 tcttaaaaga tactatccca tccgaaataa gtttttccat atgattaaat gtatctttat
    10801 ttaaacatgt aattgtcata tgaaaaccat aatcacaata acaatttcca cttgcttttt
    10861 tatgatatgc ttctacacct tgcagtaggt cttccccctc attggcttct gcaaaatcta
    10921 gtattgtagt agttcctcca gctatagctg ctcttgttcc tgtttcaaaa ttatctgcag
    10981 ttgtaataga acctgcattc atatcaaaat gagtatgtgt gtctattcca ccaggaatta
    11041 caattttacc tgtagcatca ataaccttat caccatttat ttctaagtta tttcctattt
    11101 caactatttt ttcatcttct atccttaaat cacttatata agtcttatca gaggtaatta
    11161 tagttccacc tcttagtatt gttcccatat atacctccta acttttaagc tttaaagtat
    11221 tttctaagta ttgtaaaact ttatttaaaa ttgtattttc taaaggtgct atattcagca
    11281 cctctagact atatttaaat aatttattgt gaactaaata ttattttccg tatacagatt
    11341 ttaatattcc atagtaacct tctgttccta taaataactg ttcttgctct atatactcat
    11401 ctattgtgtg agcaagattt tccttagaag gaccaaatcc tatagttttt attccagctt
    11461 cacctgcata gtgacttcca ttcgtacaaa atgagtattg agttatttct gaatctattc
    11521 cagcttcttt taatccctta tatgctgctt gaacaaattc atcttcttcg tcatataacc
    11581 aacctgggaa gaatctttca ctttctatag tatttcctgt atagcaatca gctttttcaa
    11641 c
//
LOCUS       DAELLV010000116        11638 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.116, whole genome shotgun sequence.
ACCESSION   DAELLV010000116 DAELLV010000000
VERSION     DAELLV010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11638
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.116"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            800..1831
                     /locus_tag="KPD58_002318"
     CDS             800..1831
                     /locus_tag="KPD58_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBG0266080.1"
                     /translation="MENICDMNNKVKVAVLDTGIDKEHDYLKDNLVGGIAFECMHDYI
                     FISDKFDDEDGHGTACASIIKKEYEDVELFVIKILRKDGITNIKVLEEALRYLLDTNI
                     RLINLSLSVIGVESVKGLFEVCYELFRKGKIIVCSLANDFDLSYPAMFNNVIGVRGFT
                     LDGDDSFWYNKKYDVQCVMDSNSYISCDINNSYRLPPKCNSYLSAKLTGKIAKILSEK
                     PGITISDLNDKLESLATRNHWDSCDFDECSRIVNFKLDLYDKENALFMKVADVVRDCL
                     DIEENNEKLFKGSLFNKEIGLVYDNCFNLLKKLEHRFDIKFNYMDISKYDLVSIYTLT
                     ELVERYKNL"
     gene            1964..3262
                     /locus_tag="KPD58_002319"
     CDS             1964..3262
                     /locus_tag="KPD58_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBG0266081.1"
                     /translation="MKYEEALEYISQTNKFGIRLGLENIGKLLELLGNPQETLNIIHV
                     AGTNGKGSVCSFVSNILRECGYKVGLYTSPYLETFTERIRVNGQNIPQDDVARIIELI
                     KEKIEIMVKEGYAYPTEFEVVTAMAFYYYSEQKVDFVALEVGLGGRYDATNIITKSLV
                     SVIVSISLDHTGILGDTIEKIAYEKAGIIKENGVVLVYDQTDEAKDVIKSVCKEKKAK
                     YIEVDFDDINIKKSDINSQIYDCTVMKETYRDLEIKLIGEHQINNSILAISVIKYLKD
                     INKLANINEESIRKGLINTKWPGRIEKIKENPIFIIDGAHNEDGAKSLAKALDKNFKG
                     RKLTLLIGMLEDKDIDSVLEILLPHFNKVITTTPNNPRAINSDILREKVLKYVDDVTS
                     KHEIEDAVNYTLETSSEDDIIISAGSLYMIGTVRTLVKKL"
     gene            complement(3376..3621)
                     /locus_tag="KPD58_002320"
     CDS             complement(3376..3621)
                     /locus_tag="KPD58_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HBG0266082.1"
                     /translation="MKVTFNPSGVCCREMIFEVDENNVIVDAEFIGGCNGNLSGLRSL
                     IIGQNALEVADKLNGIDCGGKGTSCPDQLSKAIRQSI"
     gene            3769..4635
                     /locus_tag="KPD58_002321"
     CDS             3769..4635
                     /locus_tag="KPD58_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HBG0266083.1"
                     /translation="MKIYISNYLSKSISIVDYSTLELEKEIVLEDNIYPHHFCIEKEK
                     NLIYIPSSSNGILYVLDLSNDKIIDTVSIGGSLSQVVLSDNELFVANEDSNSIYVLDK
                     NTLNPIGIIGVDNMPHGFDFDRESKKLYVPCINSIVCIDTINKNIQKKINMDFRAWHI
                     ELDKQKKEMYISTLDGKLIIIDEVSMDIKKVLYEFLLPVEIRFNYSGKKVYVADLGYN
                     NVRILDYTTSRYIGNIEINGIPQGLEISKDEKLLFVSDTQENSVKVYETANNKLIKEI
                     KVGKEPTTIICL"
     gene            complement(4639..5175)
                     /locus_tag="KPD58_002322"
     CDS             complement(4639..5175)
                     /locus_tag="KPD58_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HBG0266084.1"
                     /translation="MFENSSEELAYHKLLILYILEKIKMDLTNSQITQVVLETEMMNY
                     FSLQQLLSQLMESKFLTTYKDSDREYYALTQRGVESLEYFFNRIPTSVTEKIDKYIDS
                     NKENLLADTQVKSSFVKQSDNEFIVNLRVIENQSNLIDLNLNVSSEKQAKLICNNWKN
                     NASYMYAEVIDLLIRDIH"
     gene            complement(5366..5569)
                     /locus_tag="KPD58_002323"
     CDS             complement(5366..5569)
                     /locus_tag="KPD58_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HBG0266085.1"
                     /translation="MSNSNRTVVPEAKAALNQMKLEIANEIGLSNYENIDKGNLTARE
                     NGYVGGYMTKKLVEMAERQMAGK"
     gene            5860..6624
                     /locus_tag="KPD58_002324"
     CDS             5860..6624
                     /locus_tag="KPD58_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG0266086.1"
                     /translation="MKREVKRTKALIYSVLFFSLLLVLIFVFVYGIKYICSKSDNSKM
                     PIYKVDTNEKKIALSFDVAWGTDNMDDILKILDKHNVKATFFLVGSWVDDNEEIVKAI
                     DEKGHEIGNHSNTHANLKEISKEDITKEIETTSEKIYNITGKKTNLFRPPFGDVNNKA
                     MDICSDLGYKVIKWDVDSIDWKELGPNHVIEKVIKESQPGSIVLFHANINDVDNYLDT
                     IISRLKKDGYSLVKISDLLYKDNYVVDSNGVQKTKN"
     gene            7013..8326
                     /gene="prdC"
                     /locus_tag="KPD58_002325"
     CDS             7013..8326
                     /gene="prdC"
                     /locus_tag="KPD58_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase-associated electron transfer
                     protein PrdC"
                     /protein_id="HBG0266087.1"
                     /translation="MNKLYSILLKQHVGGPDKPVVSVGDVVKKGTLIAEPTGLGANIY
                     ASVSGKISEINDQAIVIEADEVQEDTFEPLKGEGILDLIKEAGVVGMGGAGFPTHVKL
                     NIDLNGGTILANAAECEPLLAHNIKEIEERPEIVYQGIKYAMEVTNAGKGMLAIKSKH
                     PKAIEAFKKVIKPGDNIEVAELVDMYPMGEERAIVRDVLGKLLEPTQLPSEANAVVIN
                     VETLTRIVEAVEQKRPVISKNITVVGQLNSGKESIVFEDVPIGTTVGELIERAGGIKG
                     EYGEIILGGPFTGKATTLDAPITKTSGGIIVTMPFVNEKRKMGLLVCACGPNEERMRD
                     IATKMGVTDIVSVQKCKQAQEIKGALKCENPGHCPGQAQKCIEFKKAGAEVILIGNCT
                     DCSNTVMGSAPKLKLGVYHITDHVMRTVNHPLIRRIKGDVRLKIK"
     regulatory      8729..8814
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            9396..11504
                     /locus_tag="KPD58_002326"
     CDS             9396..11504
                     /locus_tag="KPD58_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HBG0266088.1"
                     /translation="MKTKIKKSSIISLVIAFSMVFTAFTPIVSYADEVTSNDTILNGE
                     EQSNGQTPDVEKPSDGQVPDGEKPLDGQIPDGEKPSDGQIPDGEKPSDGQIPDGEKPS
                     DGQIPDGEKPSDGQMPDGEKPSDSQTPDVEKPSDGQMPDGEKPLDEQIPEEEKPLEEE
                     IVIEEMSLKQDIDKILDMTLSQINKIVYNFWEDEEDVKADEQSEINQVFTSEDSFISL
                     WYDKKAKVKNSCLLKEIDGSDRPYHDLRFDDITKTLTFDYVIKGLVGASSKDDKYVIE
                     GEDGEQTAFCYNNHLRPPSSNGKSPYLPAEDFNGQENQNEEAVKSILYAGSEFDGFGY
                     KQQFNLGEEENDIMTYSATQSAIWIILGQIDEQERLKQYQGSIDLCDKLIEIAKTEEE
                     RAEYQKRKEVAINTKAYLEALLKAGREELKPNDTGKPSLSNGLTKINFEKNEDGTYET
                     EAVALVGYSGVVKLKLPDGVTAYDVDGNIIGTGEVEISTQQKFKLKSVGKPDAKANIS
                     AVSYDYIFPKAIQYYKAVLDLGQKDHSSSLPSSKQNLLSYTIEKKNGQEVNFNIGLPT
                     DDVVNPPVPPTDDTNGHKPKPSPPIDDTVINPPVPPMDDTIINPPVPPTEDAVLNPPV
                     PPTEDAVLNPPVPPTEDTVLNPPVPSVSDTVEKTPELSRDDTIIKSPKTGDETQLMSY
                     VFISVIAICGLAYQCKIKRN"
BASE COUNT         4700 a         1275 c         1972 g         3691 t
ORIGIN      
        1 tttttagaat atgattacct ttttaaaata atgactattt ataaaactct atagatattt
       61 gtttcattta gctatgcata ctttaattaa ttatattaat atttaatgga gtacaatata
      121 aatattaaag cgacatctta tagtttattg aacctatagt aatgaattaa taaatagttt
      181 aattctatga aaagatttcc ttttatgcaa tttatttatt gaaatatgat ataatatata
      241 aaggtgtaag tattagttgt taaaataata tttatattag tttttatgca tacttggtat
      301 aagtttttca tatattgatt aaaattgatg ttgatttatt atttttagta ttttttcacc
      361 ttttgattca tactttgtag aatggtgtat tttctaaaac tgattataag acaagttcgt
      421 tcaaattagc atttttatag catataaata attgttcccc ctaaataaga tttagatgta
      481 tagtattaaa ataaaaaagt ataaaataaa attgatgctt aatcatagat aaatattaat
      541 aaaatataca aggggattgt ctaataaaaa gataatctcc ttttttacaa ataattctaa
      601 attgtgatat aaatctaaaa attttatctt aacatttaac catttaatga taataattga
      661 tataataaat ataaattaaa tatatggtgt acttatttta tgtttttagc aagaaatata
      721 gcatttaaaa gataaattgt gcaacttaaa tcaaagttta agatgaacta ttaacataga
      781 tatattaaaa acttttgtaa tggagaatat atgtgatatg aataataaag ttaaggttgc
      841 agtattagat acaggaatag acaaagaaca tgattactta aaagataatt tagttggagg
      901 aattgctttt gaatgtatgc atgattatat ctttatatca gataaatttg atgatgaaga
      961 tggtcatgga actgcttgtg catcaataat aaagaaagag tatgaagatg tagagttatt
     1021 tgtaataaaa attttgagaa aagatggtat tacaaatata aaagttcttg aagaagcatt
     1081 aagatacctt ttagacacta acataagatt aattaattta agtttatcag tcataggagt
     1141 agaaagtgtt aagggcttat ttgaagtttg ttatgaattg tttagaaaag gaaaaataat
     1201 agtatgttca ctcgctaatg attttgactt aagttatcca gctatgttta acaatgttat
     1261 aggtgtaaga ggatttactt tagatggaga tgattcattt tggtataata aaaaatatga
     1321 tgttcaatgt gttatggata gcaattcata tataagttgt gatataaata attcctatag
     1381 attaccacca aaatgtaata gttatttatc agcaaagctt actggtaaaa tagctaagat
     1441 attatcagaa aaaccaggca taacaatatc tgatttaaat gataagcttg aatcattagc
     1501 aactagaaat cattgggata gttgtgattt tgatgagtgt agtagaattg taaactttaa
     1561 attggatttg tatgacaaag aaaatgctct ttttatgaaa gttgctgacg ttgttaggga
     1621 ttgcttagat attgaagaga ataatgaaaa actctttaag ggtagccttt ttaataagga
     1681 gataggcttg gtttatgata attgttttaa tctgttgaaa aagctagagc atagatttga
     1741 cataaagttt aattatatgg acatctcaaa atatgactta gtttcaatat atacactgac
     1801 tgaattagtg gaaagatata aaaacttata aataaagatt tgtagctaaa tttaatgtag
     1861 aaggtattaa attatatgta ttctttgtcg gtagattaaa agtaaaaaat atactataat
     1921 tagatagcat aagagaaaat gacaatacag ggggagttag ataatgaaat atgaagaagc
     1981 attagagtac atatcacaaa ctaataaatt cggtataaga ttaggattag aaaatatagg
     2041 aaagttatta gaattattag gaaatccaca agaaactttg aatataatac atgtagctgg
     2101 aactaatgga aaaggttctg tgtgttcttt tgtttctaat atactaagag agtgtggata
     2161 taaagttggt ttatatacat ctccatattt agaaactttt actgagcgta taagagtaaa
     2221 tggacaaaat ataccacagg atgatgttgc tagaataatt gagttaataa aagaaaaaat
     2281 agagattatg gtaaaagaag gctatgcata tccgacagaa tttgaagtgg ttactgcaat
     2341 ggcattttat tactattcag aacaaaaggt agactttgtg gcattagaag ttggtttagg
     2401 tggcagatat gatgctacaa atataataac aaaatcactt gtaagtgtga tagtatctat
     2461 aagtttggac catacaggta tacttggaga tacaatagaa aaaatagcgt acgaaaaggc
     2521 tggaataata aaggaaaatg gagtagtact agtatatgac cagactgatg aagcaaagga
     2581 tgttataaag tcagtttgta aagagaaaaa agctaaatat atagaagttg attttgatga
     2641 tataaacata aaaaaatcag atataaattc tcaaatatac gattgtactg taatgaaaga
     2701 aacttataga gaccttgaaa taaaattgat aggcgaacat caaataaaca attctatctt
     2761 ggcaattagt gtaataaaat atctaaaaga cataaataaa ttagcaaata taaatgaaga
     2821 atctataaga aaaggactta taaatactaa atggccagga agaatagaaa agataaaaga
     2881 aaatccaatt tttataatag atggtgcaca taatgaagat ggagcaaaat ctttggcaaa
     2941 agcacttgat aagaatttta aaggtagaaa attaacctta ttaataggta tgttagaaga
     3001 taaagatatt gatagtgttt tagagatatt gttgccacat tttaataagg taataacaac
     3061 aacaccaaat aacccaaggg ccataaattc agatatatta agagaaaaag tattaaaata
     3121 tgtagatgat gtaacatcaa agcatgagat agaggatgca gtaaattata cattagaaac
     3181 atcaagtgag gatgatatta taataagtgc aggttcatta tatatgattg gtacagtgag
     3241 aactttagta aagaaattat aattttataa aataagtata caaataaaaa tataaaaaag
     3301 agttgtctaa aaaatggaca actcttttta tataaaataa gtttttaata attttactga
     3361 tgtttatata agtaattata tagattgacg tatagcttta gataattggt ctgggcaaga
     3421 agtacctttt ccaccacagt caataccatt taatttatca gcaacttcaa gagcattttg
     3481 gccaattatt aagcttctaa gtcctgataa attaccatta catcctccta taaactcagc
     3541 atcaacaatc acattatttt catcaacttc aaaaatcatt tctctacaac atacaccact
     3601 tggattaaat gttactttca ttaaaaagac ctccattaaa tattatttct ttttttaaca
     3661 ttaataataa taccaaatat atgcgtaata atgcaactat aaagcataat acaagctaat
     3721 gaaataatat attattgtag tatatgtatt gtagagagga ggggttatat gaaaatctat
     3781 atttctaact acttatcaaa gagtattagc atagttgatt attctacact tgaactagaa
     3841 aaagaaatag tattagaaga taatatatat ccacaccatt tttgcataga aaaagaaaaa
     3901 aatttaatat acatacctag ttcaagtaat gggatactct atgtcttaga tttaagtaat
     3961 gataaaataa ttgatactgt ctctatagga ggaagtctta gtcaagttgt acttagcgat
     4021 aatgaattgt ttgtggcaaa tgaagattct aatagtatat atgtactaga taaaaacaca
     4081 ttaaatccaa taggaattat tggagttgat aatatgccac atggatttga ctttgataga
     4141 gaaagcaaaa agttatatgt accgtgtata aactcgatag tatgtataga tacaattaat
     4201 aaaaatatac aaaagaaaat aaatatggac tttagagctt ggcatataga acttgataaa
     4261 caaaagaaag aaatgtatat atcaacactt gatggaaagc ttataataat agatgaagta
     4321 agcatggata taaaaaaggt attatatgaa tttttattac cagtagaaat aagatttaat
     4381 tatagtggca aaaaagttta tgttgctgat ttgggttata ataatgtaag aattttagat
     4441 tatacaacga gtagatacat tggaaatatt gaaataaatg ggattcccca aggattagaa
     4501 atatctaaag atgaaaagtt actatttgta tcagatacac aagaaaattc agtaaaagtt
     4561 tatgaaactg caaataacaa attaataaaa gaaatcaagg taggaaaaga gcctacaact
     4621 ataatttgtt tgtaggcttt aatgtatgtc tcttataagt aaatcgataa cttcggcata
     4681 catataagat gcattatttt tccagttatt gcaaataagt tttgcttgtt tctctgatga
     4741 aacatttaga tttaaatcta ttaagttaga ctgattttct atgactctaa ggttgacaat
     4801 aaattcatta tcactttgtt ttacaaaact agactttact tgagtatctg ctagtagatt
     4861 ttctttgtta gaatctatat acttgtctat tttttcagta acactagtag gtattcgatt
     4921 aaagaagtat tctagacttt ctacgcctct ttgagtaaga gcataatatt ctctgtcaga
     4981 gtctttatat gtagtcaaaa acttagattc cataagctga gataaaagct gttgtaatga
     5041 aaaataattc atcatctcag tctctaatac tacttgagtt atttgagaat tagttaaatc
     5101 cattttaatt ttttctaata tatataaaat taaaagtttg tgataggcta attcttcaga
     5161 cgagttttca aacataaata tcatcctccg tcaaactatt tctatatata atatattaaa
     5221 ctaatttgta caaaattaaa agaacaattt gtattgtatt attaatcttt aataattcat
     5281 ataaaaacaa ttttataaat aaatatagag aaacagggct gctaattagc agccctgttg
     5341 atacccaggt gtcaaaattt actatttatt ttccagccat ttgtctttca gccatttcaa
     5401 ctaatttctt agtcatatat ccaccaacat atccattttc tctagctgtt aagttacctt
     5461 tgtctatgtt ttcatagtta gataaaccta tttcattagc tatttctaat ttcatttggt
     5521 ttaaagcagc ttttgcttct ggaactactg ttctgtttga atttgacata gtaaatttcc
     5581 tcctcaaata attagtttat gatgtctaac aacactttta tttatgtgat gttaatacta
     5641 atttctccaa aataaaaata atatatacag aaaaataaag gtaaaaatgt aaaaaatata
     5701 aaaaaattaa ataaatgagt attttagaga gtttaattag gtcaatatag gttttatttt
     5761 tttagttatt gtcttataaa ttaaatatta cagtactttt ttgagctgaa aaaaaataaa
     5821 ttttaaatat agaagaaatt atttcaaagg gggaatagta tgaaaagaga agttaaaagg
     5881 actaaagctt tgatatatag tgttttattt ttttcattat tattagtgtt aatatttgtt
     5941 tttgtttatg gaataaaata catatgttct aaatctgaca attcaaaaat gcctatatat
     6001 aaagttgata caaatgagaa aaaaatagct ttgagttttg atgtagcatg gggaactgat
     6061 aatatggatg atattttaaa aattttagat aaacataatg taaaagctac cttcttttta
     6121 gttggtagtt gggtggatga taatgaagaa attgtaaaag ctatagatga aaaaggacat
     6181 gaaattggaa atcattcaaa cactcatgcc aatttgaagg aaatatcaaa agaagacata
     6241 acaaaagaga tagaaactac atcagaaaaa atatataata taacaggaaa aaagactaat
     6301 ttatttagac ctccttttgg ggacgtaaac aataaagcta tggacatttg tagtgattta
     6361 ggttataaag taattaagtg ggatgtagat tctatagact ggaaggagct tggaccaaat
     6421 catgtaattg aaaaagtaat aaaagaatct caacctggtt caatagtttt attccatgct
     6481 aatataaatg atgtagataa ctatttggat actataataa gtcgactaaa aaaagatgga
     6541 tacagtctag tcaaaatatc tgatttgttg tataaagata attatgtagt tgattccaat
     6601 ggagtgcaaa aaactaagaa ttaataagta ataaatgtat attttaagta aagttgtgat
     6661 aaattatata gtatatgtta gaaaagtgtc gataaaaaga cggtgtctat tttttgacac
     6721 tttttataca tgtatgcaat atcaataatt atagattaaa ttgattaaaa aagtgtctaa
     6781 taattgactt ttttttgatg ggtttaaaaa tttagaagaa ttaatgaatg ctgtaagaca
     6841 gtatttccaa agggtataag tttgtttaaa agttggcata gaaattgctt tgttaataag
     6901 cgatatgttc tatagaaata aacaatatgt taagacctta taatagaaaa atagcattaa
     6961 cattatttga acagcttata aaaaaattaa tatacttaag ggggcgtact agttgaacaa
     7021 actatattct attttattaa aacaacacgt tggtggtcca gataagccag tagtaagtgt
     7081 tggagatgta gtaaaaaaag gaactctaat cgcagaacca actggattag gggcaaatat
     7141 ttatgccagt gttagtggaa aaataagtga aataaatgac caagcgatag taattgaagc
     7201 tgatgaagta caagaagata cttttgaacc attaaaagga gaaggtatac ttgacttaat
     7261 taaagaagct ggagtagtag gaatgggtgg agcaggattc cctactcatg taaaattaaa
     7321 catagattta aatggtggaa caatattagc aaatgcagct gaatgtgaac ctttattagc
     7381 tcataatata aaagaaatag aagaaagacc agaaatagtt tatcaaggta taaaatatgc
     7441 tatggaagtt actaatgctg gaaaaggtat gctagcaata aaaagtaagc atccaaaagc
     7501 aatagaagct ttcaaaaaag taataaagcc aggagacaac atagaagttg cagaattagt
     7561 tgatatgtac ccaatgggag aagagagagc aatagtaaga gacgttctag gaaaattact
     7621 tgagccaact caattacctt cagaggctaa tgcagtagta ataaacgtag aaacactaac
     7681 tagaatagta gaggctgttg agcaaaaaag accagttata tctaaaaata taactgtagt
     7741 tggtcaatta aacagtggaa aagaatctat agtgtttgaa gatgtgccaa ttggaacaac
     7801 tgtcggagaa ttaattgaaa gagctggtgg aataaaaggt gaatatggtg aaataatcct
     7861 aggaggacct ttcactggta aagctactac tttagatgct ccaataacta aaactagtgg
     7921 tggtataata gttactatgc catttgttaa tgaaaagaga aaaatgggtc ttttagtttg
     7981 tgcatgtgga cctaatgaag aaagaatgag agatatagca actaaaatgg gagttactga
     8041 tatagtatca gttcaaaaat gtaaacaagc tcaagaaatt aaaggtgcat taaaatgtga
     8101 aaatccagga cactgtccag gacaagctca aaaatgtatt gaatttaaga aagctggagc
     8161 agaagttata ttaataggaa actgtactga ctgtagtaac acagtaatgg gttctgcacc
     8221 aaaattaaaa ttaggtgtat atcatattac tgaccatgta atgagaacag ttaatcaccc
     8281 attaataaga agaataaaag gtgacgtaag attaaaaata aaataataga taaatagaag
     8341 ttagacatct ttagaagtaa aacttaaaag ttacttctaa agatgttttt taatgtattt
     8401 tgagggcaat atatattgta aaataaatgg aaaaaataga caaaatatgc tagatattgt
     8461 ataatgataa caatagctat tacaattaat attggacaaa aataatgata ctcaagtatg
     8521 tggtttgtat taatatgcat attatattaa gtcaactatc cgatagagta aaaggttttt
     8581 gtgatatttg aagatactct atagtatctt ttttattata aaaactaatt tttgtctttt
     8641 attagataag tatctaggat actaatttat attttattga gttgttaaaa aagtgcactt
     8701 aaaaagttaa aaaagaaaaa ttaaaaaacc tatttataga aactgtgaag tatatcttaa
     8761 acctgggcac ttaaaagata tatggagtta gtagtgcaac ctgctataaa tataaatagg
     8821 agtaactttt aattgtcaaa aatatataaa ataaatacat aaataagtaa ttaatagaag
     8881 atagatataa atatattttt gaaatgctcc ctgcattcat aacgagtgta gagtggcaga
     8941 tatctgccca aaactccttt aattgctgga agttcctaaa gctaactaaa ccacaacata
     9001 atcatgaaat aaggataagt gtgatagagt gtgcatttaa taagcacttg aggttacgaa
     9061 agtagaaaaa attagttaga tagcataagg ttaaatccta agtgccttga aaatggataa
     9121 tcagcaggta agcttcgaaa agaagaaact tcaacgacta gagagaaatc tcgtacctac
     9181 aagtgtgggg aaatggggag tacctaattt atggttaggt aatgatatag tctgtgctta
     9241 atagaaatat taagaagttc ataggagaac tggttaggag taacgaccct aattgaacac
     9301 actaaagatg tattattgaa taagattcat ataatattta ataattttaa ttatattaag
     9361 tgaatacaat atatgttttt agtatcagga gaattttgaa aacaaaaatt aaaaaatcaa
     9421 gtataatcag tttagtaatt gcttttagta tggtatttac tgcatttaca ccaatagtat
     9481 cttatgctga cgaagtgact agcaatgata caatacttaa tggagaagaa caatcaaatg
     9541 gtcagacacc agatgtagag aaaccatcag acggtcaggt accagatggg gagaaaccat
     9601 tagatggtca gataccagat ggagagaaac catcagatgg tcagatacca gatggagaga
     9661 aaccatcaga tggtcagata ccagatgggg agaaaccatc agatggtcag ataccagatg
     9721 gggagaaacc atcagatggt cagatgccag atggagagaa gccatcagat agtcagacgc
     9781 cagatgtaga gaaaccatca gatggtcaga tgccagatgg ggagaaacca ttagatgagc
     9841 aaattccaga agaagaaaaa ccattggaag aagaaatagt tatagaggaa atgagtttaa
     9901 aacaagatat tgataaaatt cttgatatga ctttatctca aataaataaa atagtatata
     9961 acttttggga agatgaagaa gatgtaaagg cagatgaaca atcagaaatt aaccaagttt
    10021 ttacttcaga agactcattt attagcttat ggtatgacaa aaaagcaaag gtgaaaaatt
    10081 cttgtttact aaaagaaata gatggttcag atagacctta tcatgattta agatttgatg
    10141 atattacaaa gactttaact tttgactatg taataaaggg tcttgtagga gcaagctcaa
    10201 aagacgacaa atatgtaata gaaggtgaag atggagagca aactgcattt tgttacaata
    10261 atcatttaag accaccaagt agtaatggaa agagtccata tttaccagca gaagatttta
    10321 atggtcaaga aaaccaaaat gaagaagcag ttaaatctat actatatgca ggttctgaat
    10381 ttgatggatt tggatataag caacaattta atttaggcga agaagaaaat gacataatga
    10441 cttactcagc tactcaaagt gcaatttgga ttattcttgg acaaatagat gaacaagaaa
    10501 gacttaagca gtatcaaggc tctatagacc tttgtgataa acttatagaa atagcaaaaa
    10561 ctgaagagga aagagctgag tatcagaaga ggaaagaagt agcgataaat acaaaagctt
    10621 atttagaagc attgttaaaa gcaggtcgtg aagagttaaa gccaaatgat acaggaaaac
    10681 ctagtttgag taatggttta actaagatta actttgaaaa aaatgaagat ggtacttatg
    10741 aaacagaagc tgtagcctta gtaggttata gtggtgtagt aaagttaaaa ctaccagatg
    10801 gagtaactgc ttatgatgta gatggtaata taataggtac aggcgaagta gaaatttcaa
    10861 cgcaacagaa atttaaactt aagagtgttg gaaaaccaga tgccaaagct aatatttctg
    10921 ctgtatctta tgattatatt tttccaaaag ctattcaata ttataaggca gtgcttgatt
    10981 taggacaaaa agaccattca tcttcattac catctagtaa gcaaaattta ttaagttata
    11041 caatagagaa aaaaaatggt caagaagtta actttaatat aggtctacca acagatgatg
    11101 ttgtaaatcc accagtgcca ccaacagatg atacaaatgg acataagcca aaaccatcac
    11161 caccaataga tgatacagta ataaatccac cagtaccacc aatggatgat acaataataa
    11221 atccaccagt accaccaacg gaggatgcag tgttaaatcc accagtgcca ccaacggagg
    11281 atgcagtgct aaatccacca gtgccaccaa cagaggatac agtgttaaat ccaccagtac
    11341 catcagtaag tgatacagtg gaaaaaactc cagaactatc aagagatgat acaataataa
    11401 aatcaccaaa aacaggtgat gaaactcagc taatgtcata tgtatttatt agtgtcattg
    11461 caatttgtgg attagcgtat caatgtaaaa taaaaagaaa ttaatctaga ttattattaa
    11521 gtttaaaata aaaagtctca aattgattta taagtcattt tggggctttt ttatgtaaat
    11581 ataatatgca aatatagttt gtttaaaaat atatatttta attatattct taaattta
//
LOCUS       DAELLV010000117        11612 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.117, whole genome shotgun sequence.
ACCESSION   DAELLV010000117 DAELLV010000000
VERSION     DAELLV010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11612
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.117"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..2486
                     /locus_tag="KPD58_002327"
     CDS             <1..2486
                     /locus_tag="KPD58_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sporulation-associated two-component system
                     sensor histidine kinase"
                     /protein_id="HBG0266089.1"
                     /translation="CIYLFNSLGIKTKSIMEINITISAITDLFPILGVYLSFKFVKDN
                     KQIYSSVILFIATATLTITSLFIISGICDYLGNGKSQYALGVIISIFIIIISIVSGIE
                     LNSSSNKGKWEYDEKKIFNRIIVIIILSRVPNLLHIVIDNRNVERILSQIIINVALYY
                     LYNYIVSKNIKKTALELHDTNEELTKKTESLKEKNKKLIYETEKIEELKGILMKRESR
                     LQSTLDVAVNSIVVFSKDGEITYANKSFRNTFGDYKEQDKLTDKIKNFNDLIESIHYV
                     FVHEKNAEKLISTSDNKVYQAIFAPLIIASQTEGVLCVLEDKTKKKEYERKLIEANKR
                     SKDFLESIGDGIVVLEGNNKIYVNNACREIFKNNLESIDFSLVCKSEESIEKRYVIDG
                     EVKYVEMSFSQYTNAGTNKTIIVIRDTTSRKISQIKLEESQSSYSRFIDILPDGICLL
                     KEDLSINYANKSFLDMLAFTNIDDIKDSNIKLLMNANTEEKMKFTDNMAKVLNENRSI
                     LLLEYELINSYDDIVEVELSALPFAIYNTRYIMLIIKDLVHKKYSEQAEKELLERFKT
                     DKIKTEFFANMSHELKTPLNVISSSNQLVDSFYRNEKIKDYNNNIKSHVDLVRQSSYR
                     LQRLINNIIDLTKMESGFYTLKLAKYNIVSVIEDLFMNIEEYALRKDIKILFDTDLEE
                     INVYIDKVEIERIMLNLLSNCIKFTDNGGWIYVSIHYKIDKVIISVKDTGVGIPQDKL
                     ELIFEEFSQVDKTLSRNTEGSGIGLAIVKNLVSLHGGDIEVVSEVNKGTEFLISLPMK
                     GFSSEHYTEDKRIYNIQEKIKIEFSDIYY"
     gene            complement(2605..3549)
                     /gene="manA"
                     /locus_tag="KPD58_002328"
     CDS             complement(2605..3549)
                     /gene="manA"
                     /locus_tag="KPD58_002328"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HBG0266090.1"
                     /translation="MEPLFLKPIFMDRIWGGTALKDKFNYEIDSPTTGECWAISSHKN
                     GDCLIENGKYKGKKLSELWNKNRELFGNTPGDKFPLLTKILDANDNLSVQVHPNDEYA
                     KKNENGELGKTECWYVIDCSDDAEIIIGHNAKSHKEFVNMVNNNEWDKLLRKVNIKKG
                     DFFYVPSGTIHAICKGTLILETQQNSDTTYRVYDYDRTDNSGNKRELHVQKSIDVTNV
                     PHINFDTDYKIVSTSDFKCTTFVSNEFFSVYKLDVFGKCNFTHNTPFSLYSVLDGNGK
                     LIHNSVEYNLKKGMHFILPNDFGDFSFEGNLEIICSHI"
     gene            3722..5083
                     /locus_tag="KPD58_002329"
     CDS             3722..5083
                     /locus_tag="KPD58_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG0266091.1"
                     /translation="MELFKAESKKYKKEFLKLLEQWVSIPSFYDRKTVSKDMPFGKGV
                     YDALNWFENLGRENNFKVKNIDNHAVQIEYGNGKEYVDIFGHCDVVNPGEGWDSEPFK
                     LNIIGDKLVARGVSDNKGPMIVNFLALKMIKDLDINLKRKVRLIAGGNEESGFKCIKH
                     YYSKEPYGVCGFTPDAKFPVLNGEKGGAIIKLISNIDDKSLYISGGIEFNTIPDKVYI
                     KNVEKLGKDNICFDINNISINYDNGNYIVYGKGGHSSKPEKSINPILATIKLLSENID
                     EKWTKDLYKLINQDNINGNLFGLNIEGKCGILSMVPTIINIVDGKLEVVLSVRYPEIL
                     TIEDIIKKFNLYMEQNNINKFELIGENLKQANYIDRNSKLVRSLHDIYIKYSGDLKND
                     VRVTSAGSYASEMNNSVIFGCEFPDGSFGNVHSANEFASLDRFITAIGIYAEAIITLC
                     NKI"
     gene            5171..7060
                     /locus_tag="KPD58_002330"
     CDS             5171..7060
                     /locus_tag="KPD58_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG0266092.1"
                     /translation="MITKKHAIIVKSLSNKDGYMTSNELAIKLDVSTKTIKRYIADLN
                     SVLSKYDLVIASSRGIGYKLSGSKNNIARAVKEANKYIDGFLDDSEESRMSNIICMLI
                     NRNYMSIEAMAEELNLSIAAINKLSSKLKRKLEKYDLVIKSKPYYGSYIHGEEINIRQ
                     LITDYAIKLDEKSKVKVFLDDISENDIHCIESILEKHLREKDTIISDKDFNLLLSKII
                     VSVFRSKRGHSNNINLMDTSYRFHNYYFIENLMKEISDKIGFKLIEDEVIYISNYSGV
                     IAYKGTQGRKNTSEIEERISTVISSALQDIFLISGSDYTKDDEFMVAISDHIKRFLNR
                     ARANVKSNNPLLHQIKEKFPIAFNLAVFLSNKLETEFNLKLDEDELGYIAIHFAASNE
                     RMKKNTSKKICIVCHYGIGTGQLISEKLKQNISDLSVVGVYPVRYLDMAISQDVDLIV
                     STVELKGYEKPVLYIENIFDDSLIENVNKAFYEKEERRKIISNMFDEKAFFSIKANTK
                     EEVIISLSNKLKERDFIEESSIKSIIDRENISSTEIGNLVAIPHTIVKGDKKSIIGVG
                     ILENPIIWDKQEVQLVFMVFFNTKEKHNFSIFKYLYNFIKDEGGVRGIIKICDFNKLM
                     ALIGN"
     gene            7075..7530
                     /locus_tag="KPD58_002331"
     CDS             7075..7530
                     /locus_tag="KPD58_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0266093.1"
                     /translation="MGTNIFNKEYVFLNVDAKSKVEVLKFISKKAKDLNLAEDESLVY
                     EGLMAREEQFTTNLGESIAIPHTKNDAIENPAVVVLKFNEDVVWNEGEDKVKLAISLL
                     MPGKSKENIHLKLLSSLSRKLINKEFKDSLLKSDNVEEISNLINEALGL"
     gene            7589..7906
                     /locus_tag="KPD58_002332"
     CDS             7589..7906
                     /locus_tag="KPD58_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBG0266094.1"
                     /translation="MNKKLVALCACPMGLAHTFMAAEAIEQAAKALGYEAKVETQGAD
                     GVQNELTRDDILGATMIIHAVAITPEGMERFDGCEVYEVELQEAIKNAEGVIKEIEED
                     LGI"
     gene            7931..9193
                     /locus_tag="KPD58_002333"
     CDS             7931..9193
                     /locus_tag="KPD58_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBG0266095.1"
                     /translation="MAIKKKVINSSGASTTGGNNVGKSTPSSPTQSKGNGKWKEISKH
                     IMTGISYMIPVLVMGGLIGALSQLIPYAILGLDPSVGIVDAMNSGEFTGFKLSLLNIA
                     QLMSNFGFTLFGFAIPLFAAFCANSIGGKTALIAGFIGGYIANKPVGVPQFVDGQWTE
                     VVPVASGFLGAILIAFIIGYFVKWLNKSIKVSHNWLAFKTTFLIPLIASLACMVLMIF
                     IITPFGGLINESMKNFLTAAGAAGEYVYATALAAATAFDLGGPINKAAGFVALGLTTE
                     NVLPITARTIAIVVPPIGLGLTTLLDKRLVGRRVYDRQFYQAGKTSIFLSFMGISEGA
                     IPFALERPGFVIPLNIVGSVIGAITGIILGAIQWFPESAIWAWPLVDNLFGYIIGIAV
                     GAIFIAVGNIFYRNKLIKDGKLVVDYID"
     gene            9298..10362
                     /locus_tag="KPD58_002334"
     CDS             9298..10362
                     /locus_tag="KPD58_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG0266096.1"
                     /translation="MDKIEKVREYFREYDIDGFLINSPTNKFYVGNLFSSSGYVFITK
                     ESQYIIVDFRYFEEIKRKSSLFNVVLMDKTRTHFDIINDICREQNIKEIGFEGNEVSF
                     DLYRSMSNKLSATLKSVDLSTLRETKNEDEIKYIKKACEIVDATFYHIVDFIKVGMTE
                     KQVENEIVRIIKDLGGQKESFDTIVASGLRGALPHGKASEKVIEYGDFVTFDFGAKYN
                     NYCSDITRTICMGTINKELEEIYNIVRKANEECIRVLRPGMTTGEIDKVARDIIGSYG
                     YANNFGHNLGHGVGIMVHEYPALAPESNEVLKEGMIVTIEPGIYVPSLGGVRIEDDVL
                     ITQDGCMRLTTSTKDLIVVS"
     gene            10416..11594
                     /locus_tag="KPD58_002335"
     CDS             10416..11594
                     /locus_tag="KPD58_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HBG0266097.1"
                     /translation="MYNFDDKPDRVSEKCRKWDLNIIRDKFGDIREDFIPMWIADMDF
                     KIPTEIENKFIEAVRRGVFGYTYCYDEFYDSVIGWQKNMHEVNVEKEWITLTYGTVST
                     LHYVVQAFCKKGDSIILNTPVYDPFESSAKKQGVNVICNTLDVVNDRYYINFDKLEVQ
                     IKENKPKLMMFCTPHNPSGRIWTIEEMTRVATICKENNVILVADEVHAEHIHYGTFNS
                     ILKIGKELLENVILLTSPNKGFNLGGLKTSYSIVINKDIRDKFRNKLKQNSITSPNVF
                     GIIGLITAYNECHEWLSGVNEYIKSNYELLETWVNKYNKIKLMKMESSYLAWMDISGL
                     GISASEFTDKLAIDTGVLLEDGSHFVKDGEKYVRINLGTQKENVIEALNRMDLFLKSL
                     "
BASE COUNT         4676 a         1171 c         2020 g         3745 t
ORIGIN      
        1 agtgtatata tttatttaat tccttgggga ttaaaactaa gtctataatg gagattaaca
       61 tcacaatatc agcaataaca gatttatttc ctatactggg tgtatactta agttttaaat
      121 ttgttaagga taacaaacaa atttattcaa gtgtaatttt atttatagca actgctacat
      181 taacaataac atctttattc ataatatcag gaatttgtga ctatctggga aatggtaaat
      241 cacaatatgc tttaggagtt ataatatcta tattcataat aattatttct atcgttagtg
      301 gaattgaatt aaattcaagt tcaaataaag gtaaatggga atatgatgaa aaaaagattt
      361 ttaacagaat aatagtaata ataatcttat caagagttcc aaatctattg catatagtta
      421 ttgataatag aaatgtagag aggatattat cacaaataat aataaatgta gccttatatt
      481 atttatataa ttacatagta tctaagaata ttaagaaaac agcactggaa ttgcatgata
      541 cgaatgaaga attaacaaaa aaaacggaaa gtttaaaaga aaagaataaa aaattaatct
      601 atgagacaga aaagatagag gaacttaagg gaatacttat gaaaagagaa tctaggctac
      661 aatctacatt agatgtagca gtaaatagca tagtagtatt tagcaaagat ggagaaatta
      721 catatgcaaa taagagcttt agaaatactt ttggagacta taaagaacaa gataaattga
      781 ctgataaaat aaaaaacttc aatgatttga tagaaagcat tcattatgtg tttgttcatg
      841 agaagaatgc agagaaactt attagtacaa gcgataataa agtttatcaa gctatatttg
      901 cacctttaat tattgcttca caaacagaag gagtattgtg tgtactagaa gataagacta
      961 agaaaaagga gtacgaacgc aaacttattg aagcaaataa gagaagtaaa gattttttag
     1021 aaagtattgg agatggcata gtagtgcttg aaggtaataa caaaatatat gttaataatg
     1081 catgtaggga aatttttaaa aataatttag agagtattga tttttcatta gtatgtaaaa
     1141 gtgaagaaag tatagaaaaa agatatgtaa tagatggtga agtaaagtat gtagagatga
     1201 gtttttctca gtatactaat gctggtacta ataaaactat tattgtaata cgtgatacaa
     1261 cctctcgaaa aatatctcaa ataaaacttg aggaaagtca atcatcatat tcaagattta
     1321 tagatatatt acctgatggc atatgcttat taaaagaaga tttaagtata aactatgcaa
     1381 ataagtcgtt tttagatatg ctagctttta caaacataga tgatataaaa gattctaata
     1441 ttaaactatt aatgaatgcg aatactgaag aaaaaatgaa atttacggat aatatggcta
     1501 aagttttaaa tgaaaataga agtatacttt tactggaata tgaattgata aatagttatg
     1561 atgatatagt agaagtagaa ttaagtgcat taccttttgc tatatacaat actagataca
     1621 taatgcttat tataaaagat ttagtacata agaaatattc cgagcaagca gaaaaagaac
     1681 tgcttgaaag atttaaaaca gataaaatca aaacggagtt ttttgcaaat atgtctcatg
     1741 aactaaaaac tccactaaat gttatatcta gttcaaatca actggtagat tctttctata
     1801 gaaatgaaaa gataaaagat tacaataaca atattaaatc tcatgtggat ttagttagac
     1861 aaagttctta taggcttcaa agattaataa ataacattat agatttaaca aagatggagt
     1921 ctgggttcta tacactaaaa cttgcgaaat acaatattgt atctgtaata gaagacttat
     1981 ttatgaatat agaagagtat gcacttagaa aagacataaa aattcttttt gatacagacc
     2041 ttgaagaaat taatgtttat atagacaagg ttgaaattga gagaattatg ttgaatctat
     2101 tgtcaaactg tattaagttt actgataatg gaggctggat atatgtaagt attcattata
     2161 aaatagataa agtaatcata agtgtaaaag acactggagt tggaatacca caagataaat
     2221 tagagttgat ttttgaagaa ttttcacaag ttgataagac tttatccaga aatactgaag
     2281 gaagtggaat agggcttgct atagttaaga atctagtaag tcttcatggt ggagatatag
     2341 aagtagtaag tgaagtgaat aaaggcacag agttcttaat ttcacttcca atgaagggat
     2401 ttagtagtga acattacaca gaagataaga gaatatataa tattcaagaa aaaataaaaa
     2461 tagaattttc tgatatatat tattaaaaat gattatgagc aaaaataaaa taaaaatact
     2521 atttgtttaa atatttaagt tgtaagaaat aggctatcat aaggtaaatt attatgatag
     2581 cctattttta gctaggtttt atatctatat gtgagaacat ataatctcta aattaccttc
     2641 aaaactaaaa tctccaaagt cgtttggtag tataaaatgc ataccctttt ttaaattgta
     2701 ttctacagaa ttgtgtataa gttttccatt tccatccaac acactgtata aagaaaaagg
     2761 tgtattgtgt gtaaaattac attttccaaa aacatctaat ttataaacac taaaaaattc
     2821 gtttgacaca aatgttgtgc atttaaagtc tgatgttgat acgattttat agtcagtatc
     2881 gaaatttata tgaggaacat tcgtaacatc tattgatttt tgaacatgaa gttcacgctt
     2941 attacctgaa ttgtcagttc tatcataatc atagactcta taagtagtat ctgagttttg
     3001 ttgagtttct aaaatcaatg ttcctttgca tatagcatgt atagtgccac ttggaacata
     3061 gaaaaagtct ccctttttta tattaacctt tctaagtagt ttgtcccatt cattattatt
     3121 taccatattt acaaattctt tatgagattt tgcattatga cctattataa tttctgcatc
     3181 atcagaacaa tctattacat accaacactc tgtcttacct aactctccat tttcattttt
     3241 tttagcatat tcatcatttg gatgaacttg aactgataaa ttatcatttg catccaatat
     3301 ttttgtaagt aaaggaaact tatcaccagg tgtgtttcca aataattctc tatttttatt
     3361 ccacaattca gatagttttt tacctttata ttttccattt tctattaagc aatctccatt
     3421 tttatgagag cttatagccc aacattcacc agttgttggc gaatctattt cataattaaa
     3481 tttatcttta agtgcagtac caccccatat tctatccata aaaataggtt ttaaaaataa
     3541 tggttccata atcctgttcc tcctatgata ttaaatttat cctacaaata attatatata
     3601 aatagattat aaaatcaata gtaaaaaagt tttgtccctt gaaatgacat aaagtataag
     3661 gagttattaa ataagataaa ctatgatata taatataaag ttctaaattg gagtgataat
     3721 tatggagttg tttaaggcag aaagcaaaaa atataaaaaa gaatttttaa aattattgga
     3781 gcaatgggtt agtatacctt cattttatga tagaaaaact gtatctaaag acatgccttt
     3841 tggaaaagga gtatatgatg cattaaactg gtttgaaaat ctaggtagag aaaacaattt
     3901 caaagtaaaa aatatagata atcatgctgt tcaaattgaa tatggaaatg gtaaagagta
     3961 tgtagatata tttggacatt gtgatgttgt aaatccaggt gaaggatggg atagtgaacc
     4021 atttaaacta aatattatag gtgataaatt agttgctaga ggtgtatctg ataataaagg
     4081 gcctatgata gtgaattttt tagcactaaa aatgataaag gatttagata ttaatttaaa
     4141 aagaaaggtt agattaatag ctgggggaaa tgaagagagt ggatttaaat gtataaagca
     4201 ttattatagc aaagagccat atggtgtatg tggatttact ccagatgcta aatttcctgt
     4261 attaaatgga gaaaaaggtg gagcaataat aaagttaatt tcaaatatag acgataaatc
     4321 tttatatata agtggtggaa tagagtttaa tacaatacca gataaagttt atataaagaa
     4381 tgtagaaaaa ttgggtaaag ataatatatg ttttgatatt aacaatatat cgataaatta
     4441 tgataatggc aattatattg tgtatggaaa aggtggtcat tcatctaaac ccgaaaaatc
     4501 aataaatcct atattagcta caattaaact tttgtcagaa aatattgatg aaaaatggac
     4561 aaaagattta tataagttga taaatcaaga taatataaat ggaaacttat ttggtttaaa
     4621 tattgagggg aaatgtggaa ttttatcaat ggtgccaaca ataataaata tagtagatgg
     4681 aaaattagaa gtagttttaa gcgttagata tccagaaata ttaaccattg aagatataat
     4741 aaaaaaattt aatttgtata tggagcaaaa taatattaat aaatttgagc ttattgggga
     4801 aaatttaaag caagcaaatt atatagatag aaattcaaaa cttgtaagaa gtttgcatga
     4861 tatatatata aagtattctg gagatttaaa aaatgatgtt agagtaacta gtgcaggaag
     4921 ttatgcttct gaaatgaata attcagtaat atttggttgt gaatttcctg atggtagctt
     4981 tggcaatgtc catagtgcga atgaatttgc tagcttagat agatttataa ctgcaatagg
     5041 tatatatgca gaggcaataa ttacattatg taataaaata taaatacaaa atgtccctta
     5101 tatagacata ttatatatac aattaaaatt ttacttgata tataattgaa atcaaagaga
     5161 aggtgataca atgataacta agaaacatgc aataatagta aagagtttaa gcaataaaga
     5221 tggatatatg acatcaaacg aattagctat caaattggat gtgtctacta aaactattaa
     5281 aagatatata gcagatttaa atagtgtttt aagcaaatat gatttggtaa tagcttcttc
     5341 tagaggtata ggatacaagt taagcggttc taaaaacaat atagccagag ctgttaaaga
     5401 agcaaataaa tatattgatg gttttttaga tgactctgaa gagtcaagaa tgtcaaatat
     5461 aatatgtatg cttattaata gaaattatat gagtatagaa gcaatggcag aagaattaaa
     5521 tctaagtata gccgctataa ataaattgtc tagtaagctt aaaagaaaac ttgaaaagta
     5581 tgacttagtt ataaaatcta aaccttacta tggaagctat atacatggtg aagaaattaa
     5641 tataaggcaa ttaattactg actatgcaat aaaattagat gaaaaaagta aagtgaaggt
     5701 gtttttagat gacattagtg aaaacgatat acactgtata gaaagtattt tagaaaagca
     5761 cttaagagaa aaagatacaa taatttcaga taaagatttt aatcttttgc tttcaaaaat
     5821 aatcgtttct gtatttagga gtaagagagg tcatagtaac aatataaact taatggatac
     5881 aagttataga tttcataact actactttat agaaaatcta atgaaagaaa tatctgataa
     5941 aattggattt aaacttattg aagatgaagt aatttatatt tcaaattatt ctggtgtaat
     6001 agcctataaa ggtacacaag gaagaaaaaa cacaagtgaa attgaggaac gaataagtac
     6061 tgttataagt agtgcacttc aagatatatt ccttatttca ggtagtgatt atacaaaaga
     6121 tgatgagttt atggttgcaa tctctgacca tataaagaga tttttaaata gagctagagc
     6181 taatgtaaaa tcaaataatc ctttacttca tcaaataaaa gagaagtttc ctatagcttt
     6241 taatttagcc gtgtttttat caaataaact agaaactgag tttaatttaa aacttgatga
     6301 agatgagctt ggatatatag ctatccactt tgctgcttca aatgaaagaa tgaaaaaaaa
     6361 tactagtaag aaaatttgta tagtttgtca ttatgggata ggaacaggac aacttatttc
     6421 tgaaaagcta aaacagaata taagtgattt aagtgttgtg ggtgtttatc cagttagata
     6481 tctcgatatg gctataagtc aagatgttga tttgatagta tctaccgttg aattaaaagg
     6541 atatgaaaaa cctgtcttat atatagaaaa tatatttgat gatagcttaa ttgaaaacgt
     6601 aaacaaagcc ttttatgaaa aggaagaaag aagaaagata ataagcaaca tgtttgatga
     6661 aaaagctttt ttcagtataa aggctaatac taaagaagag gtcataattt cactttcaaa
     6721 taaactaaaa gaaagagatt ttatagagga aagttctata aaaagtataa tagatagaga
     6781 aaatatttca tcaactgaaa taggaaatct agtagcaata ccccacacaa tagtgaaggg
     6841 agataaaaaa tctataatag gagttggaat acttgaaaat ccaataattt gggataagca
     6901 agaggttcag ctagtattta tggtattttt taatactaaa gaaaaacata atttctcgat
     6961 atttaaatac ctttataatt ttataaaaga tgaaggcgga gtaagaggaa ttattaaaat
     7021 atgtgacttt aataaattaa tggcactgat aggtaattaa ggaggaaatt aataatggga
     7081 acaaacatat ttaacaaaga atatgtattt ttaaatgttg atgctaagtc aaaggttgaa
     7141 gttttaaaat ttatatcaaa aaaagctaaa gacttaaact tggcagaaga tgagagctta
     7201 gtatatgaag gtttaatggc tagagaagaa caatttacta caaatttagg agaatctata
     7261 gcaatacctc acactaaaaa tgatgcaatt gaaaatccag cagtagtagt attgaagttt
     7321 aatgaagatg ttgtttggaa tgaaggtgag gataaggtta aattagcaat aagcttactt
     7381 atgccaggaa aaagtaaaga gaatattcat cttaaattat tatcatctct ttcaagaaaa
     7441 ttaataaata aagaatttaa agattctctt ttaaaaagtg ataatgttga agaaatatct
     7501 aacttaataa atgaagcttt aggtttataa ttaaacacta aggttataaa ataaaaaaat
     7561 aattaactaa aaataggagg aatttgaaat gaataaaaaa ttagtagcat tatgtgcttg
     7621 tccaatggga ctagctcaca ctttcatggc agcagaagca atagagcaag cagcaaaggc
     7681 attaggttat gaagcaaaag ttgaaactca aggtgctgat ggtgtacaaa atgagttaac
     7741 aagagatgat atattaggag caactatgat aatacatgca gtagcaataa caccagaagg
     7801 tatggaaaga tttgatggtt gtgaagttta tgaagttgaa ttacaagaag ctataaaaaa
     7861 tgcagaaggt gttataaaag aaatagaaga agatttagga atataataaa tttattgtgg
     7921 aggaaataga atggcaatta agaaaaaagt tataaacagt tcaggtgctt ctacaacagg
     7981 aggaaataat gtaggaaaat ctactccatc tagtccaact caatcaaagg gaaatggaaa
     8041 atggaaagaa ataagtaaac atataatgac aggtatatca tatatgatac cagttttagt
     8101 aatgggtgga cttatagggg ccttatctca attaatacca tatgctatat taggacttga
     8161 cccatctgta ggtatagttg atgctatgaa ctcaggagaa tttacagggt tcaagcttag
     8221 tcttttaaat atagcacaat taatgtcaaa tttcggattt actttgtttg gatttgcgat
     8281 accactgttt gcagcatttt gtgcaaactc tataggtgga aaaacagctt taattgcagg
     8341 atttataggt ggatatatag ctaataaacc tgtaggtgta ccacaattcg ttgatggaca
     8401 atggactgaa gttgttccag ttgcatcagg atttttaggt gcaatactaa tagcatttat
     8461 aataggatat tttgtaaaat ggttaaataa atctataaaa gtttctcata actggttagc
     8521 ttttaaaaca acatttttaa taccattaat agcgtcatta gcttgtatgg tattgatgat
     8581 atttataata actccatttg gtggattaat taatgaaagt atgaaaaact tcttaacagc
     8641 agcaggagca gctggagaat atgtatatgc aacagctttg gcagcagcta cagcattcga
     8701 cttaggagga ccaataaata aagcagcagg attcgttgca cttggtttaa ctacagagaa
     8761 tgtacttcca ataacagcaa gaacaatagc tatagttgtt cctccaattg gattaggatt
     8821 aacaacatta ttagataaaa gattagtagg aagaagagta tatgatagac aattctatca
     8881 agcaggaaaa acttctatat tcttatcatt tatgggtata tctgaaggag caataccatt
     8941 tgcacttgag agaccaggtt ttgttatacc tttaaacata gttggaagtg taataggagc
     9001 tattacaggt ataattctag gagcaattca atggttccca gagtcagcta tatgggcatg
     9061 gcctctagta gataacttat ttggatatat aataggtata gcagttggtg ctatattcat
     9121 agcagtagga aatatattct atagaaataa attaataaaa gatggtaaac ttgttgtaga
     9181 ttatatcgac tagtatttaa tttttactat ctataaataa ctttggctgg taatatggtt
     9241 ttaatcaata ttaccagcct tacctatatg attaattaaa ggcggtggag ataagatatg
     9301 gataagatag aaaaagtaag agagtatttt agagaatatg atatagatgg atttcttata
     9361 aatagtccta caaacaagtt ttatgttgga aatttattta gctcttcagg ttatgttttt
     9421 ataactaaag aatcacaata tataatagta gattttagat attttgaaga aattaaaaga
     9481 aaatctagtt tgtttaatgt agtattaatg gacaaaacta gaactcattt tgatataatt
     9541 aatgatattt gtagagagca gaatataaag gaaattggat ttgagggaaa tgaagtatct
     9601 tttgatttat atagaagtat gagtaataag ttaagtgcaa ccttaaaatc agttgattta
     9661 tctacattaa gggaaacaaa aaatgaagac gaaataaaat atataaaaaa agcttgtgaa
     9721 atagttgatg ctacatttta tcatatagta gactttataa aggttggtat gactgaaaag
     9781 caagttgaaa atgaaattgt tagaattata aaagatttag gtggtcaaaa agaatctttc
     9841 gatacaatag ttgcatctgg gcttagagga gcattacctc atggaaaagc aagtgaaaaa
     9901 gttatagagt atggagattt tgttaccttt gactttggtg ctaagtataa taattattgt
     9961 tcagatatta ccagaaccat ttgtatgggt actataaata aagagttaga agaaatatat
    10021 aatatagtaa gaaaagctaa tgaagagtgt ataagggttt taagaccggg aatgactact
    10081 ggagagatag ataaagttgc aagagacata attggttcat atggatatgc caataatttt
    10141 ggtcataatc tgggtcatgg tgtaggaatt atggttcatg aatatcctgc attagctcct
    10201 gaatcaaatg aagtattaaa agaaggaatg attgtaacta tagagcctgg tatatatgtt
    10261 ccaagtcttg gaggcgtaag aatagaagat gatgtactta taacacaaga tggatgtatg
    10321 agattgacta catctactaa ggatttaata gttgtaagct aaaaataaat tagataaata
    10381 ttttataaca ataagaaaag aagaggtaaa tttaaatgta taattttgat gataaaccag
    10441 atagagtaag tgaaaagtgt agaaaatggg atttaaatat tataagagat aaatttggag
    10501 atattagaga agattttata ccaatgtgga ttgctgatat ggattttaaa ataccaacag
    10561 aaatagaaaa caaatttatt gaagctgtaa gaagaggtgt atttggatat acatactgtt
    10621 atgatgaatt ttatgattca gttattggtt ggcaaaaaaa tatgcatgag gtaaatgtag
    10681 aaaaagaatg gattacatta acttatggaa ctgtatcaac acttcattat gtagtacaag
    10741 ctttttgtaa gaaaggtgat agtataatat taaatacacc agtttatgac ccctttgaat
    10801 catcagcaaa aaaacaaggt gtaaatgtaa tttgtaatac attagatgtt gtaaatgata
    10861 gatattatat aaattttgat aaattagaag ttcaaattaa agaaaataag cctaaattaa
    10921 tgatgttttg tacaccacat aatccatctg gaaggatatg gacaattgaa gaaatgacaa
    10981 gggtagcaac tatatgtaaa gaaaataatg ttattttggt tgcagatgaa gtacatgcag
    11041 agcatattca ttatggtaca tttaattcaa tacttaagat tggaaaagag cttttagaaa
    11101 atgttattct attgacatca ccaaataaag gatttaatct tggtggatta aagacttctt
    11161 attcaatagt tataaataag gatataaggg acaaatttag aaataaatta aagcaaaatt
    11221 ctataacttc tccaaatgtg tttggaataa taggattgat aaccgcttat aatgaatgtc
    11281 atgagtggtt aagtggtgta aatgaatata taaaatcaaa ttatgaactt ttagaaactt
    11341 gggtaaataa atataataag ataaaattga tgaaaatgga atcatcttac ttagcttgga
    11401 tggatataag tggattaggt attagtgcaa gtgagtttac agataaattg gctatagata
    11461 caggagtatt attagaagat ggaagtcatt ttgtaaaaga tggtgagaag tatgttagaa
    11521 taaatttagg aactcaaaag gaaaatgtaa ttgaagcctt aaatagaatg gatttatttt
    11581 taaagtcttt ataaaaattt ggttgaatta gt
//
LOCUS       DAELLV010000118        11514 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.118, whole genome shotgun sequence.
ACCESSION   DAELLV010000118 DAELLV010000000
VERSION     DAELLV010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11514
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.118"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            56..1438
                     /locus_tag="KPD58_002336"
     CDS             56..1438
                     /locus_tag="KPD58_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HBG0266098.1"
                     /translation="MLSKNIASKVLEKCLITGGDFAEIFEEDTINNSISLIDNKVENA
                     IGGRNYGIGIRIFKGLKSIYAYTSDNSLNSLLDVAYKAAVALGKLEDGKSVILNDSIK
                     INNIHNIKIYPNSIGNKDKVSVMKVAYKSAKEFSNDISQVSVSYLDKDQKILIANTEG
                     IYVEDRRIRTRLGISSIASKGNENQTGFEGPGGCKGFEIFEEIDPEYYAKESARVAHT
                     MLHAKNCPAGNMMVAIDNGFGGVIFHEACGHSLEATSVAKGNSVFADKLGQQIASTKI
                     TAIDDGTIPNYWGSMNIDDEGNPTQKNILIENGILKSYMIDKLNGRRMGMNPTGSSRR
                     QNYKFAPTSRMTNTYIAAGEDKPEDIIKSIPDGLYAKKMGGGSVNPVTGEFNFAVSEG
                     YLIKNGEIQEPVRGASLIGKGSDILMNIDMIGNNVKQAQGMCGSSSGSIPTNVGQPMI
                     RVKEITVGGR"
     gene            1450..2799
                     /locus_tag="KPD58_002337"
     CDS             1450..2799
                     /locus_tag="KPD58_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HBG0266099.1"
                     /translation="MELKNFKDILFKKALSEGFEECEIYYYTGENLSINIYEGEVEKY
                     NLDKSFGLSFRGKVNGKIGYSYTEILDDKAVDMLIKNVKDGVNTIENEDVQFIYEGDK
                     HYNDVKTYSKELENLEADKLIDLALEMERETKAYSDKVVNLSKCTISYSVSSNGISNT
                     KGLNLSNKTNMLIGFVVPIIEDNGQKYDGIGYNIANSIEEIKPCEIAKLGVDNALSKV
                     GGKSIPSGKYKTILLNEAMVSLLSTFSGIFNADSAQKGLSLLKNREGDMIASPIVTIV
                     DDPLLENGMASTPFDDEGVATFKKEVVLNGKLITLLHNLKTANKAGVKTTGNGIKSSY
                     SSPISIYPTNFYIEKGDKSLDEIIKDIDEGLMVTSFAGLHSGANSVTGDFSLAAKGFY
                     IKEGKKVFPVEQITVAGNYFDLLKDIEAIGEDLEFPMSSIGSPSVVIKELSVAGKDE"
     gene            2949..3482
                     /locus_tag="KPD58_002338"
     CDS             2949..3482
                     /locus_tag="KPD58_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBG0266100.1"
                     /translation="MSDAHYDMHKRNGYSSDEPYPEIKVLGPNKYYAELLMDDYAGVS
                     SEFTSVNQYLYHNFDLDETHRELSEMWINISITEMLHMEILAKTIRLLGGNPVYRGST
                     SSCGAYWNGGFVCYGNSICNRLKLDLHLEHVAINNYYKDISLIEDPYIKAILNRIILD
                     EKLHVSLFEKAIEKYCK"
     gene            3566..4375
                     /locus_tag="KPD58_002339"
     CDS             3566..4375
                     /locus_tag="KPD58_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0266101.1"
                     /translation="MYYVYVNDINIAVYDLNPSAKKTVLFIHGWPLGHKIFEYQTNIL
                     PKLGYRTVSIDLRGFGKSDATSGGYTYSQLADDIYKVVHAIGLKDFTLVGFSMGGAIV
                     LRYMSLFNGYGVSKLVLAAAAAPSFVQRPPEFPYGMTREDVNKLIAQACTDRPEMVTD
                     FGEKVFASNPPESFRRWFNDIGFSASGIGTIGTAVSLRDEELFNDLKCVRVPTGIFHG
                     KLDEICPYEFAVFMNEKIEDSILYTFEYSGHAIFYDELKLFNQEFLQFLEE"
     gene            4786..6831
                     /locus_tag="KPD58_002340"
     CDS             4786..6831
                     /locus_tag="KPD58_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0266102.1"
                     /translation="MKKHNILFVSTDDKINIDISKQLENIFGEFCNIDNLVYVNRINI
                     ELSSYELVVCSDNDIKEYIHNNIDKNIPIVIVHRTINIENINQIISIENDSDVMVIDA
                     YKESADETAKIIRKLGLIHINLIPYYPGCDKSKCEIGIITGSRNSIPQNIKQIIDIGD
                     KVIDINTVIEIFTKLNISIDKLHIIKEKYNEDTVSGYRYYTTMNKTMKSFLEIIDEGI
                     ASVDKLGKFIYCNKVFSNLVGIDQNEIISNNFMDLFSDKVVKKIFFQEDEVNDEVVNL
                     NNKKLIINKVNVYENNERIKSIISIKNISAIQVLEDKIQNKLQSKGFVSKYTFESVVG
                     ESKIIKEKINIARKIATTDFSVLILGENGTGKEIFAQAIHNESLRKNKPFVAVNLSSL
                     SDTLIESELFGYDEGSFTGAIKGGKMGIFERAHTGTIFLDEIGDISLDVQQRLLRVLQ
                     EKEVMRMGGSKIIPIDVRIIAATNKDLKKKIKEGSFREDLYYRINVLHIEIPRLRERK
                     EDIPLISKYFLDEINSNKCFTEESMQALTLYEWPGNVRELKNLIYYIDTIVEEDRVDY
                     EHLPEQFRFEKNNTLVNENFDSIILDFKQSNFFEESICILTSIETWNNKNILLGRNKL
                     QEILKEKDIVLSVDQIRKRIDKLKSHGLLLSGVKKQGSFITDEGKNFISYVKIKGVI"
     gene            7092..8291
                     /locus_tag="KPD58_002341"
     CDS             7092..8291
                     /locus_tag="KPD58_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HBG0266103.1"
                     /translation="MKSAYKGYEAYSYLEKGKDISSIEMCKGDKRVKEYLIELDDIQE
                     AKVKSIVDEKIFISLHEHPVLFPEHLERDIFEYNREGKQRCAYEALSRGYYDAIFDNL
                     MDGVCTITSNSGWKWDDILIDLGMRLCDLAHQDFVIKCEKVEDIYRAKSEGKVALIPT
                     LEGAAPIENELDRIDILYGFGIRLMGITYSESNALGSGLKEEKDGGLTNFGKKAVERM
                     NKIGMAIDCSHVGVQTTLDVIEYSKDPIILSHVGARNLWNTKRLTPDEVLIACAKKGG
                     VIGVEAAPHTTITEKNKEHSIESFMEHFEYIKNLVGIDHVAFGPDTVYGDHVKLHSVL
                     AAKFDIKKITGTKEHPKVEYVKGLENPTETSKNIVRYLVKHGYSDEEIEKVLGGNILR
                     VLKQVWK"
     gene            8343..9038
                     /locus_tag="KPD58_002342"
     CDS             8343..9038
                     /locus_tag="KPD58_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HBG0266104.1"
                     /translation="MDYLKLANHPLLWIASTVAVSIVVVQSLIFAIKSCKVGKTMGIT
                     DKQIKSAVKSSAISAIGPSMTIFAGMVSLIVTMGGPIAWMRLSFIGSVIFESMSAGFG
                     TDALGITLGSPEMTKLAFTNAVWTMILGSLGWIIFTLLFGHKLDKITNVISSGKKSFI
                     PIISLGAMLGSFAYLNADRVLRFDNGTIACISGMAIMVILCMLEKKKNIKWLREWGLT
                     ISMFSGMIIASVI"
     gene            9055..9732
                     /locus_tag="KPD58_002343"
     CDS             9055..9732
                     /locus_tag="KPD58_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266105.1"
                     /translation="MSKVKSIYNEEYLPFMIRYGRLTLSLGIIAALVPGIILSFGFGI
                     MPPISALLASTMAIVSMSAPNYIIEPVSYSPILGIPGTYMSFLSGNISNMRLPCSIAA
                     QKAAEVESGTEEGSIISTIGIAVSILVNISILTIGVILGGSVLSKIPAEVVEKLNLIL
                     PALFGSVFGQVFLQDKKLGLVAIVISVLTIILSKQGIIPQSLVVLICVFGTILIARAM
                     YKDKLSD"
     gene            9843..11444
                     /locus_tag="KPD58_002344"
     CDS             9843..11444
                     /locus_tag="KPD58_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG0266106.1"
                     /translation="MIYDLILKNGFIIDGTGNPGFYGDIAIKDNLIAKIDSKINSNTN
                     KEIDCCGKVITPGFIDPHVHEEIVAILDGKFEKFLKQGVTTTINGNCGHSITPYSSEN
                     VYEYMYKNGLLLEEEKKYLIDKNKCWNNFTEYCDLISKSGISVNMGFLLGHGTIRWSV
                     MGGSKDRPPTEKEKNEITDIINDGMKSGAFGISTGLAYIPSKYADIDELVDIARQIKE
                     YDGIYTSHIRDYIGRYNAVKEAIEVGQKSGARVQVSHLSPVEIEAFDEILKARYNGVE
                     IMVDTVPRSSGHCMKKKRVIQFIMAISSSLFELGIDGVMDALRNEEGRTLILKEAFIL
                     GDRGSIILLNTKDINMEKKSIREIATQKGIDEDKLLLDLLLDGDEELIFCLGGMYRAD
                     FPDKLHDDKIIDNPFVMVGSDCLFSVGGDMSWFELQRRGAFPIFFNMYRKSGVRLEEI
                     VRRVTSLPARQFKIKNRGILKEGLIADIAVIDMDNYSYPRSEDIDFSKPQLLANGVEY
                     VIVNGKIALENGEITENKCGEVLKR"
BASE COUNT         4525 a         1156 c         2196 g         3637 t
ORIGIN      
        1 caagtaaaaa gattgaataa aagttaatta aaaatttaaa gtaggaggta aatttatgct
       61 ttcaaaaaat atagcttcta aggtattaga aaagtgtcta attactggag gagattttgc
      121 tgagattttt gaagaagaca ctataaataa ttctattagt ttaatagata ataaagtaga
      181 aaatgcaata ggtggaagaa attatggtat aggaataaga atatttaaag gattgaaaag
      241 tatatatgct tatacaagtg acaattcatt aaatagtctg ttggatgtag catacaaagc
      301 tgctgttgct ttaggtaaat tggaagatgg aaaaagtgta atattaaatg acagtattaa
      361 aataaataac atacataata taaaaattta tccaaattca ataggaaata aagataaagt
      421 aagtgttatg aaggttgcat ataagagtgc aaaagaattt agtaatgata tatctcaagt
      481 aagtgttagt tatttggata aagaccaaaa aatattaatt gcaaacactg aaggtatata
      541 tgttgaggat agaagaataa gaactagact agggataagt tcaattgcat ctaagggtaa
      601 tgaaaatcaa actggatttg aaggtcctgg aggttgtaaa gggtttgaaa tatttgaaga
      661 aatagaccca gaatattatg caaaagaatc agctagagta gcacatacta tgttgcatgc
      721 taaaaattgt cctgcaggta atatgatggt agcaatagat aatggatttg gtggagtaat
      781 atttcatgaa gcttgtggac attctttgga agctacatca gttgcaaagg ggaactctgt
      841 atttgcagat aaattaggtc aacagattgc ttccacaaag attacagcta tagatgatgg
      901 tacaatacca aactactggg gttctatgaa tatagatgat gaaggaaatc caactcaaaa
      961 gaatatactg attgaaaatg gaattttgaa gtcttatatg atagataaac ttaatggtag
     1021 acgaatggga atgaatccta caggaagttc tcgtagacag aactacaaat ttgcaccaac
     1081 ttcaaggatg actaacactt atattgctgc tggagaggat aaaccagaag atattataaa
     1141 gtcaatacca gatggacttt atgctaaaaa aatgggtggt ggttctgtaa atcctgtaac
     1201 aggagagttt aattttgcag tttcagaagg ttacttaata aaaaatggag aaatacaaga
     1261 gccagttaga ggtgctagtt taattggaaa aggtagcgat atacttatga atatagatat
     1321 gattgggaat aacgtaaaac aagctcaggg gatgtgtggc tcatcatcag gaagtatacc
     1381 aactaatgta ggccaaccta tgataagggt aaaagaaatt actgtaggtg gaagatagga
     1441 gggataattg tggaacttaa aaattttaaa gatatattat ttaaaaaggc tctaagtgaa
     1501 ggttttgaag agtgtgaaat atattattat acaggagaaa atttaagtat aaatatatat
     1561 gaaggagaag ttgagaaata caatcttgat aaatcttttg gtttatcttt tagaggtaag
     1621 gtaaatggaa agataggtta ttcttataca gaaattttag atgataaagc tgttgatatg
     1681 ttgataaaaa atgtaaaaga tggagtaaac actattgaaa atgaagatgt acaatttata
     1741 tatgaaggag ataagcacta taatgatgta aagacttatt ctaaagagct agaaaattta
     1801 gaggcagata agttgattga tttagcttta gaaatggaaa gagagactaa agcttattca
     1861 gataaggtgg taaatttatc taaatgtact atatcttatt ctgtatcaag taatggtata
     1921 tctaatacta aaggtcttaa tttaagcaat aaaactaata tgcttatagg gtttgtagtt
     1981 cctatcatag aagacaatgg acaaaaatat gatggaatag gatataatat agcaaattct
     2041 attgaagaaa taaaaccttg tgagatagca aaacttggtg ttgataatgc tttatctaaa
     2101 gtaggtggta agagtattcc atcaggtaaa tataaaacta tattgcttaa tgaagctatg
     2161 gtatctttgc ttagcacatt ttctgggata tttaatgcag attcagctca aaaaggatta
     2221 tctttattga aaaatagaga aggagacatg atagcatctc ctatagttac aatagttgat
     2281 gacccacttt tagaaaatgg tatggcatct actccttttg atgatgaagg agtagctaca
     2341 tttaaaaaag aagttgtttt aaatggaaag cttataactt tacttcataa cttaaaaacg
     2401 gctaataagg caggtgtaaa gactacagga aatggaataa aatcttcata ttcatcacct
     2461 ataagcatat atccaactaa tttttatata gagaaaggag ataaatcttt agatgaaatt
     2521 ataaaggata tagatgaagg attgatggtt actagctttg caggacttca ttcaggtgca
     2581 aattcagtaa caggagactt ttctttagct gccaaaggct tttatatcaa agaaggaaaa
     2641 aaagtatttc cagtggagca aattacagta gcaggaaact attttgacct gttgaaagat
     2701 attgaagcta taggagaaga tttagaattt ccaatgagta gtataggttc accttcagtt
     2761 gttattaaag agctatcagt agctggaaaa gatgaataat taaaaagtaa ttttgtggat
     2821 aaaaggtcat gttaatttta attaatatgg ctttttatta tttaagtaga ttttattatc
     2881 taagttatat gaaaaagttg tgatgcatac aattaatatc atacattaaa tttaaaggag
     2941 gagatattat gagtgatgca cattatgaca tgcacaagcg aaatggatat tcaagtgatg
     3001 agccatatcc agagataaaa gttttaggtc caaataagta ctatgcagaa ttattgatgg
     3061 atgactatgc cggagtatca agtgaattta cgagtgtaaa tcaatatcta taccataatt
     3121 ttgatttaga tgaaacgcat agagagttaa gtgaaatgtg gataaatata tctataacag
     3181 aaatgttaca tatggagata ttagcaaaga ctataagatt attgggtgga aatcctgtat
     3241 atagaggttc tacaagttct tgtggtgcat attggaatgg tggttttgta tgttatggaa
     3301 attctatttg taacagatta aaactagatt tgcatttgga gcatgtggct attaataatt
     3361 attataaaga cataagtctt attgaagacc cttatataaa agctatatta aatagaataa
     3421 tcctagatga aaaacttcat gtaagtttat ttgaaaaggc aatagagaag tattgtaaat
     3481 aaatttgaaa atttagttat tcatcatttt attcatatat ataaaataac catcatacat
     3541 tttcttgaag ggagttgctg ttgatatgta ttatgtatat gtgaatgata taaacatagc
     3601 agtatatgat ttgaatccga gtgctaaaaa aactgtactt tttatacatg gttggccctt
     3661 aggacataag atatttgaat atcaaacaaa tatattgcca aaactcggat atagaacagt
     3721 ttcaattgat ttaagaggtt ttggtaaatc tgatgcaaca tcaggaggat atacttatag
     3781 ccaattagca gacgatattt ataaggtggt acatgctatt ggattaaaag actttaccct
     3841 tgttggtttt tcaatgggag gagcaatcgt acttaggtat atgagtttat ttaatggata
     3901 tggagtgtca aaattggtat tagctgcagc tgctgcacca tcatttgtac aaagacctcc
     3961 agaatttcca tatggtatga cacgtgaaga tgtaaataaa ttgatagcac aagcatgtac
     4021 tgatagacca gaaatggtaa ccgattttgg cgaaaaagta tttgcatcaa atccaccaga
     4081 aagctttaga agatggttta atgatattgg ttttagtgca tcaggtattg gcactattgg
     4141 aactgctgta tctttaagag atgaagaatt atttaatgat ttaaaatgtg taagagtgcc
     4201 tactggtata tttcatggga aattagatga aatatgtccg tatgaatttg cagtttttat
     4261 gaatgaaaaa atagaagatt caattttata tacttttgag tatagtggtc atgctatatt
     4321 ctatgatgag cttaaactat ttaatcagga atttttacag tttttagagg aatagatata
     4381 ttttattatc acttagaaaa agagtaatat tatgtgaaaa gatttaagct aaattttatg
     4441 atgtttttta cactaaacaa atagggtttt agaacttgta actaagttct gagaccctat
     4501 tattatatat tcctaaaatt tgttataatt tattccttgt cattcttaaa aatgtattaa
     4561 tatattaacg aatttatatt aaatttttgt ttatattcca ataatgtcaa actaaccgtt
     4621 ttagaaatta aattatatgt gaaaaaagat agcttacatt attttattat ggaaaataat
     4681 agtaaatggt acaaaatttg atttttaaga tttaaaactt ttttaaaaag tgctacaata
     4741 gaccaaatta ttactttcta tactcgttaa acaggaggtg ggactatgaa aaaacataat
     4801 attttatttg tatcaactga tgacaaaata aatatagata tatcaaaaca attggaaaat
     4861 atatttggag aattttgtaa tatagataac ttggtatatg taaataggat aaatattgaa
     4921 ctgtcaagct atgagcttgt agtatgttca gacaatgata taaaggaata tatacataat
     4981 aatattgata aaaatatacc tattgtaata gtacatcgta ctataaatat tgaaaatata
     5041 aatcagataa taagtataga aaatgattct gatgttatgg ttatagatgc ttataaagag
     5101 tcagcagatg aaactgccaa gataattaga aaactaggat taattcatat taatctaata
     5161 ccatattatc caggttgtga taagtctaaa tgtgagattg gaattattac aggaagcaga
     5221 aacagcatac cacaaaacat aaagcaaata attgacatag gtgataaggt aattgatata
     5281 aatacagtta tagaaatctt tacaaaacta aatatatcta tagataaatt acatattatt
     5341 aaggaaaaat acaatgaaga tacagtaagt ggatataggt attatacaac tatgaataaa
     5401 accatgaaga gttttttgga gattatagat gaaggtattg cttctgttga taaattagga
     5461 aaatttatat attgcaacaa agtattttct aatctagttg gaatagacca aaatgaaatt
     5521 atatctaata actttatgga cttatttagt gataaggttg tcaaaaaaat attttttcaa
     5581 gaagatgaag ttaatgatga agtagtaaat ttaaataaca aaaaactgat aattaataaa
     5641 gtaaatgttt atgaaaataa tgaacgaata aagagtatta taagcattaa aaatataagt
     5701 gcaattcaag tgcttgagga caagattcaa aataaacttc agtcaaaagg ttttgtatca
     5761 aagtatacat ttgagagtgt tgtaggtgag agtaaaataa taaaagaaaa aattaatatt
     5821 gcaagaaaaa tagcaactac agacttttct gtgttgatac ttggagaaaa tggaacagga
     5881 aaagaaatat ttgcccaggc aatacataat gaatctctaa gaaaaaacaa gccatttgtt
     5941 gcagttaatc tatcttcttt gtcagataca cttattgaaa gtgaactttt tggctatgat
     6001 gaaggaagtt ttacaggagc aataaaaggt gggaaaatgg gtatatttga aagagcacac
     6061 acaggaacaa tttttttaga tgagataggt gatatatctt tagatgtaca gcagagattg
     6121 ctaagagtat tacaggaaaa agaagttatg agaatgggcg gaagtaaaat tataccaata
     6181 gatgttagaa ttatagctgc aacaaataaa gatttaaaga aaaaaattaa agaaggttcc
     6241 tttagagaag atttgtatta taggattaat gtattacata tagagatacc tagacttaga
     6301 gaacgtaagg aagatatacc tcttataagt aagtattttt tagatgaaat aaatagcaac
     6361 aaatgtttta cagaggaatc aatgcaagct ttaacattgt atgaatggcc aggaaatgta
     6421 agagagctta aaaatttaat ttattatatt gatactatag ttgaggaaga tagagtagat
     6481 tacgagcatt tgccagaaca atttagattt gaaaaaaaca atactttagt taatgaaaat
     6541 ttcgattcaa ttatcttaga tttcaaacaa tctaatttct ttgaggaaag tatatgtata
     6601 ttgaccagta tagaaacttg gaataataaa aatatattat taggaagaaa taaactgcaa
     6661 gagattttaa aagaaaaaga tatagtttta agtgtagacc aaattagaaa aagaatagat
     6721 aagttaaaga gccatggttt acttttatct ggagtaaaaa aacaaggtag ttttataact
     6781 gatgaaggaa agaactttat atcatatgta aaaattaagg gagtgatata ggggactaaa
     6841 aaggcaaaac accctaaaaa aatccctata atggatagaa ataaataaaa ttatctttaa
     6901 gattaagata attcaaaaat attgaataaa taatctaaga agattatttt taataaaaca
     6961 gtagtaaata tcaaccttac tactgttttt attttttgca agatttatta tacaattaaa
     7021 atttatattt tggcacatca attgctacat aggaaaaata taaaaaattg taaaacttgg
     7081 aggatgttgt tatgaagtca gcttacaaag gatatgaagc ctatagttat ttagaaaaag
     7141 gaaaagatat ttcatctatt gagatgtgta aaggagataa aagagttaaa gaatacctta
     7201 ttgaattaga tgatattcaa gaagctaagg ttaaaagtat agtggatgaa aaaatattta
     7261 tatcattaca tgaacatcca gtattatttc cagaacacct agaaagagat atatttgaat
     7321 ataataggga aggtaaacaa agatgtgctt atgaagcatt atcaagagga tattatgatg
     7381 ctatatttga taatttaatg gatggtgtat gtacgataac atcaaattca gggtggaaat
     7441 gggatgatat attaatagat ttaggcatga gattatgtga tttagctcat caagattttg
     7501 tcataaagtg tgaaaaagta gaagatatat acagagctaa aagtgaaggt aaagtagcat
     7561 tgataccaac attagaaggt gcagcaccaa tagaaaatga acttgataga atagatatac
     7621 tgtatggatt tggtataagg ttaatgggta taacatacag cgaatccaat gcattaggtt
     7681 caggactaaa agaagagaaa gatggaggtc ttacaaattt tgggaaaaaa gctgttgaaa
     7741 gaatgaataa aattggaatg gcaatagatt gctctcacgt tggagttcaa actacacttg
     7801 atgtaataga atatagcaag gacccaataa tattaagcca tgttggggca agaaatttat
     7861 ggaacacaaa gagactaact ccagatgaag tattaatagc atgtgctaaa aaaggagggg
     7921 ttataggtgt agaagcagca cctcatacta caataacaga gaagaataaa gaacattcaa
     7981 tagagtcatt tatggaacat tttgagtata ttaaaaattt agtaggaata gaccatgtag
     8041 cttttggacc agatacagta tatggcgacc atgtaaaatt acactcagta ctagctgcta
     8101 aatttgatat aaaaaaaata acaggaacaa aagaacatcc caaagtagaa tatgtaaaag
     8161 gtcttgagaa cccaacagaa acttcaaaaa acattgttag atatttagta aagcatggat
     8221 atagtgatga ggaaatagag aaggtactag gtggaaatat acttagagta ctaaaacagg
     8281 tttggaaata gatatattag aaatattaat aaaatttgag gatttatagg gaggttttat
     8341 aaatggatta tttaaaatta gctaatcatc cacttttgtg gatagcgagt acagtagcag
     8401 tatcaatagt agtggtacaa tcccttatat ttgctataaa atcttgcaaa gttggtaaaa
     8461 caatgggaat aacagataaa caaataaagt cagctgttaa gagtagtgct atttcagcta
     8521 ttgggccttc aatgactata tttgctggaa tggtatcgct tatagtaact atgggtgggc
     8581 ctatagcatg gatgagatta tcatttatag gttctgtaat ttttgaatca atgtcagccg
     8641 gatttggaac agatgcatta ggtataactt taggttctcc tgaaatgaca aaacttgcat
     8701 ttacaaatgc agtttggact atgatattag gttctttagg atggataata ttcacattac
     8761 tatttggaca taaactagat aagataacca atgttatatc tagtggcaag aaatcattta
     8821 taccaataat atctctagga gcaatgttgg gttcatttgc atatctaaat gctgataggg
     8881 tgttgcgttt tgataatgga acaattgcat gtataagtgg aatggctata atggtaatat
     8941 tgtgtatgct tgaaaagaag aaaaatataa aatggttaag agagtggggg cttacaatat
     9001 caatgtttag cggaatgata atagcttcag taatataagg ggggagttag ttttatgagt
     9061 aaagtaaaat ctatatacaa tgaagaatat ttacctttta tgataaggta tggaagatta
     9121 acactatctt tgggtattat agcagcatta gttcctggaa taattttgag ctttggattt
     9181 ggtattatgc ctccaatatc agcattatta gcaagtacaa tggcaattgt cagtatgagt
     9241 gctccaaatt acataataga accagtttct tattcaccaa tcttaggtat accaggaact
     9301 tatatgagtt ttctttctgg taatatatct aatatgaggc taccatgttc aatagcagct
     9361 caaaaggcag cagaagttga atcaggtaca gaagaaggct cgataatatc tacaatagga
     9421 atagctgtat ctattttagt gaatataagt atactaacta taggggtaat tttaggagga
     9481 agcgtactat ctaaaattcc agctgaagta gtagaaaaat taaacttaat tttaccagct
     9541 ctatttgggt cagtttttgg acaagtattt ttacaagata aaaaattagg tctagtagca
     9601 attgtaatat ctgtattgac tattatatta tcaaagcaag gtataatacc acagtctcta
     9661 gtagttctta tatgtgtatt tggtactata ttaattgcaa gagctatgta taaagataag
     9721 cttagtgact aattacaatg aaataatagt gaattttcaa atatgaaatt taataatttg
     9781 attttcaaat ataaaaatta cattaagatt atttaaaaat tggtttttaa ggagggaata
     9841 ttttgatata tgatttaatc ttaaaaaatg gatttataat agatggtaca ggtaatcctg
     9901 gtttttatgg agatatagca attaaagaca acttaatagc aaaaatagat tctaagataa
     9961 atagcaatac aaataaagag attgattgtt gtggaaaagt tataacacct ggatttattg
    10021 accctcatgt tcatgaagaa atagtagcaa tactagatgg taaatttgaa aagtttctaa
    10081 aacaaggagt tacaacaact ataaatggta attgtggaca ttctataaca ccatattctt
    10141 ctgaaaatgt gtatgaatat atgtataaaa atggtctatt acttgaagaa gaaaaaaagt
    10201 atcttataga taaaaataaa tgttggaata attttacaga atattgtgac cttatatcta
    10261 aaagtggtat ttctgtcaat atgggattct tgttggggca tgggactata agatggagtg
    10321 ttatgggagg ttctaaggat agacctccta cagaaaagga aaaaaatgaa attacagata
    10381 taataaatga tggaatgaaa agtggtgctt ttggaatatc tactggattg gcatatatac
    10441 caagtaagta tgctgatatt gatgaacttg ttgatattgc aaggcagatt aaagagtatg
    10501 atggtatata tacatctcat attagagatt acataggaag atataatgca gtaaaagaag
    10561 ccatagaggt tgggcaaaaa agtggtgcta gggttcaagt atctcactta agtccagttg
    10621 aaatagaagc atttgatgag atattaaagg ctaggtataa tggtgttgaa ataatggttg
    10681 acacagtacc aagaagttct gggcattgta tgaagaaaaa aagagtaatt caatttatta
    10741 tggcaatatc atctagtcta tttgaattag gtatagatgg ggtaatggat gctcttagaa
    10801 atgaagaggg aagaacactt atattaaaag aagcatttat acttggagat agggggagta
    10861 taatacttct aaacactaaa gatattaata tggaaaagaa atcaatacgt gaaatagcta
    10921 cacaaaaagg aatagatgaa gacaagttat tactcgattt acttttagat ggagacgaag
    10981 aacttatctt ttgtttagga ggaatgtata gagctgattt cccagataaa ctacatgatg
    11041 ataaaataat tgataatcca tttgttatgg ttggttcaga ttgtttgttt agtgtgggtg
    11101 gggatatgtc atggtttgaa ctacaaagaa gaggagcttt tccaattttc ttcaatatgt
    11161 acagaaagtc tggtgttaga ttggaagaga ttgtaagaag agttacttca ttaccagcta
    11221 ggcaatttaa aataaaaaat agaggtattc ttaaagaagg gttgatagct gatatagcag
    11281 ttattgacat ggataactat agttatccta ggtcagaaga tattgatttt tcaaagccac
    11341 aattactagc caatggtgtt gaatatgtaa ttgtaaatgg aaaaattgca ttagaaaatg
    11401 gagagattac tgagaataaa tgtggtgaag tattgaaaag ataaggttct aaaaataata
    11461 ggatttagaa agtaaataaa tatctaagta agtaaagacg aagtttgaga ttta
//
LOCUS       DAELLV010000119        11280 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.119, whole genome shotgun sequence.
ACCESSION   DAELLV010000119 DAELLV010000000
VERSION     DAELLV010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11280
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.119"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            673..831
                     /locus_tag="KPD58_002345"
     CDS             673..831
                     /locus_tag="KPD58_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266107.1"
                     /translation="MKIFSFLLFVIGICLCIAGFIGHYPVFVGTTLSFLAIAMIAVRQ
                     VKTKKKMK"
     gene            complement(1118..1822)
                     /locus_tag="KPD58_002346"
     CDS             complement(1118..1822)
                     /locus_tag="KPD58_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0266108.1"
                     /translation="MEIKPLVLIVEDDKPICKFIKVSLETQNYRCVETDNGGTAISLI
                     HSLDPDLIILDLGLPDIDGIEVIGRVRACAKTNKIIVVSAREHERDKVEALDGGADDY
                     LTKPFSVTELLARVRVALRNKAQQDNINNDAPKSFEVKNLKIDYENHIVSINGEEIHL
                     TPIEYKIIELMSKYSGRVLTHKFIIDKVWGNYYESENQSLRVFMASIRRKIEKNPAQP
                     EYILTEVGVGYRMADE"
     gene            complement(1813..3420)
                     /locus_tag="KPD58_002347"
     CDS             complement(1813..3420)
                     /locus_tag="KPD58_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HBG0266109.1"
                     /translation="MKKERLQHEDLKEYDYDEDGYLSKKIIIKDFIKTILVMTIATGI
                     SVVFEKVGFNESNIILVYILGVLFATSITYGYFFGILSSVIGVLSFNFFFTHPLYTFL
                     AYRKDYPMTFCIMLMAAIITSTLTSRIKREVRLSHKREKMIRLLYENNKTLLKAKNKN
                     QIIEFCGSNLVNILNRTVIVTIADENNNLGKPSIYICNNDGSENIFHSVLEKEAIKEA
                     FRTGNPVGIGTKYCKNSSAYYFPIKGQESNVLGVIGVACFKIDIITENEKILLEAVST
                     QVALAIDRENLFEKNKKANLEAERERLRGNLLRSISHDLRTPLTSILGSSSTILENDD
                     VIDKETRVELLKNIYEDTSWLTHSVENILSMTRIDEGKLDIEKRPEVVDEIIAESILR
                     VKKFANSRDIKTNIPDEIIIVHVDVLLIEQVLVNLIDNAIRHTPKNSKIELTVKKESN
                     QVLFEVADNGKGIPNEDIGNIFNRFYTKNKSRNLERRGIGLGLEICKSIVEAHGGEIV
                     AFNNPSGGATFRFSIPMYDEEGKDKWK"
     gene            complement(3674..4852)
                     /locus_tag="KPD58_002348"
     CDS             complement(3674..4852)
                     /locus_tag="KPD58_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266110.1"
                     /translation="MEKLSDKRRVKAVSMETFVFIVLLAVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLDTVFLIMAMAVLAGALSALLSEFGVISLVNKIFKGLMRPIWGLPGASIA
                     GVVATYLSDNPAIIPFAKDKTFTQYFKKYQVPALCNLGTAFGMGLIVTTFMIAQGKEY
                     VLPAIIGNVAAIIGSIISVRIMLTFTKKYYNYDPKNDTEKQINDKGAKLEEFREIRDG
                     NVFQRTLDAILEGGKLGVEMGMAIIPGVLVVCTLVMLLTFGPSTDPATGQAVYTGAAY
                     EGIKLLPAIGDKISFIIEPLFGFTSPEAIAFPVTALGAVGAAISLVPEFIKSGAITPN
                     DIAVFTAMGMCWSGYLSTHIGMMDALDARPLAGKAILSHTIGGLCAGICAHFIFMLVG
                     "
     gene            complement(5078..5572)
                     /locus_tag="KPD58_002349"
     CDS             complement(5078..5572)
                     /locus_tag="KPD58_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBG0266111.1"
                     /translation="MHLFIEVIAAFFTALSFGVLFNMKGKNLILAGIGGSIAWFSYKF
                     LLNMGVTENLCFFIATVCFAIYCELCARIYMTPATTLSVCCLIILVPGYGIYNTMYSV
                     LTNNYIKAVEYGVSTLSCASSIALGLVFITTVFRKVNLYGVLTKIKENEKYKKSINKI
                     KQQK"
     gene            complement(5573..6334)
                     /locus_tag="KPD58_002350"
     CDS             complement(5573..6334)
                     /locus_tag="KPD58_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBG0266112.1"
                     /translation="MDIDRILNFSSNAGKAMLQSGGETYRVEETIARICQSFGIDHVD
                     VFATPTAVMASVFVDGKLHSAIKRISSRRVDLNLVHEVNSLSRAISINNLDIELCEKI
                     LDEISEDNYYSDLITIFFAGIAAATFSILFGGNVEEFIAAFTVGILTKFVIMYLSKSS
                     LNDFFVNAIGAVIIASCSILILKLGFIKTLDHLIAGAIMLLVPGLALTNALRDLLDGQ
                     LISGLAKLAEVFFVGVSIAVGMFLVLGLYFKLGGI"
     gene            7036..9870
                     /locus_tag="KPD58_002351"
     CDS             7036..9870
                     /locus_tag="KPD58_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0266113.1"
                     /translation="MLRKDKVLLSLHKLCENMTLKDLQSDKTGFTTIEIANHSSLDRS
                     NTSKELNNLYNEKKVLKISGKPILFLHASIIENLIGRTLNSDEFSINNCNELLKSSKN
                     DSEDIFSTLLGHDSSLKLPIEKAKSAILYPPKGLNTLLIGPTGVGKSTFAETMYKYAV
                     ESKIFSSESKFVVFNCAEYAENANLLLSNLFGYVKGSFTGANKDKFGIVAQADGGILF
                     LDEIHRLPPEGQEMLFLLMDKGIYRRLGESDIVHKANVFIVCATTEDIHSSLLGTFLR
                     RIPVTINLPALQDRSLKERLSLIKYFFIIESKYTNATIKVHKDVIKSLLLYDCFGNIG
                     QLKSDIQLMSARAFLNYKTGLKDNIEIDLSIVPDYIYIGLLKLNENRSNLNKLSELDY
                     MLFYEFNGNNLEVEYTKELSKYNIDTSNNGFKYSTEIPYLENDIRSMFINYMEKYSTK
                     LLSISSVTHSDYTSNEIFKVINPKIYYAVEHSLKEAEKISRNKYTKQTYIALSMHISA
                     LIENINQDTYNPKYNLYNDKIFLDKDLNIAKIVLSIIENDLQTSLPKDEVKFISMFLN
                     SMYLEPNNQSESIGVIILAHGNSTASSMCDVANSLLGTNHCNAIDMPLDVKVSTILDK
                     TIELVKECDEGKGVLLLTDMGSLIAFGELISEKTKIKTRSIEMVSTPIVLEAVRKSTL
                     PEMTLDELANSLSSLNPYIGRAAIDSTEINYPEKSREYVIITTCITGYGSAMKVADFI
                     NSSLKNISKYNINLVPHNIESFKMYKNKFSNTKILAVVGSVDFNVANTPFIHIEELLN
                     GYGLDMLNKIIEGTFNANSITNIKETNLSYNSLIINFMTQNLELLDSVKLFNYVNSSL
                     FTIIELIDENLKSKFKLGYILHCSFMIERCFKNTTFPYNNITELISKNHELYSLIREA
                     FKPTEAYFNILIPDEEIAYIIDMINEYLNK"
     gene            9913..11157
                     /locus_tag="KPD58_002352"
     CDS             9913..11157
                     /locus_tag="KPD58_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBG0266114.1"
                     /translation="MEDEILKGKIKQLTILALIFIFITPVFAFADTPPVPNSSRAALL
                     IDQETKRILFEKNIDEKMPLASLSKMMTFLLAIEAVDKNQVKETDMVKIDKSTASVGG
                     STCKLKDGDEISLGELMQGLMLVSGNDAAIAIAKHIGKTEKNFVNMMNKKAEEIGMID
                     TYYFNPNGLPIYTDPEHKEPPIENMSTAHDIVTLGKYMYDHYENQVTRITTMQVYNDT
                     KKDFTHYNTNPLLVSVPGVDGIKTGYTDNAGYCLAFSMMVPKDAKNERNHRLIGVVLG
                     DGNKKNRISSSATLLKYGKDNFHSKKIAHKGDIIETPCVDGIDDFKITVKVDKDLYGV
                     VSDNENINPKVVFKNMNYPIHKGDIVGVAKYYNDSGKFVGSVDVKSESNIGCIPLKDK
                     IKIKVAKINKKLEIKNSVCFKA"
BASE COUNT         4071 a         1814 c         1403 g         3992 t
ORIGIN      
        1 aattttgcta tatatttggc aatgatatta tacttcttta tgttttaaaa tagtacctta
       61 atgaggttct attttttagt aaatagaatt attattgtgt caatcgtaca atagagtttt
      121 gtaataccta atttaatata tttataatta attatctcat tatgtgttta taattaattc
      181 tcatataaat gacaaatctc cacctcaatt aagtattcca atcatatatg aagataatac
      241 ttcattcatt taaaaatata aaactattat atattaaaat ttcttcaata ataaattatc
      301 tagattgtta ctttgatata ttatataata tcacaaaggc tgatataaaa agacaaaaag
      361 ggcaattttt ttatatcaaa aaaaaatgtt aaattgatat tatccatata taaattgata
      421 ttatccatat agatgctata tttaacaatt ctacttactt cccttacact tattagcata
      481 tagttattaa gagtttgttg caaaacaaca cttagcacac tttaaaaagt gtggtctaat
      541 aagtaatcaa cgaagaagac ataataaagc ataaaatatg atatactttt ccacgccatc
      601 aaaactgagg attttattat taatatatgc taaaatatgc ttaataaatt aaattttaat
      661 gggagaatag ttatgaaaat atttagtttc ttgttgtttg tgataggaat atgtttatgt
      721 attgctggat ttataggaca ttacccagta tttgttggta caacattgag ttttcttgca
      781 attgcaatga tagctgtaag acaagtcaaa actaagaaaa aaatgaaata ggcaaatcct
      841 aactttacaa gttaatatta cataagtaat tagtctaaaa ttggatactt tttctattac
      901 agaaatacat catccacttt gtattttctt aaaaaaataa aatagctatg taaattcaag
      961 attagataga ttaactctct atccatttca ttgtaaaata tacatagcta tttgtattcg
     1021 atttaaaata cttagatttt cttacataac aaataatttc tatctgatta ttactcatat
     1081 tcatttagta atcactcagc aactaatcat ttagtatcta ttcatccgcc atacggtaac
     1141 ctaccccaac ctcagtcaaa atatattctg gctgagcagg attcttttca attttccttc
     1201 ttatacttgc cataaaaact ctaagcgact gattttcact ttcatagtaa tttccccaaa
     1261 ctttatctat tataaactta tgggttaaga ctcttcctga atacttagac atcaactcaa
     1321 taatcttata ttcgattgga gttaaatgta tttcttcacc attaattgac acaatatgat
     1381 tttcataatc tatctttagg tttttaactt caaaagactt tggagcatca ttatttatat
     1441 tatcctgttg agctttattt ctaagagcca cacgtactct agccaaaagc tctgttacac
     1501 taaaaggttt agttaaataa tcgtctgctc ctccatctaa agcttccacc ttatccctct
     1561 catgctctct cgctgaaaca acaattattt tattagtctt tgcacatgcc ctaactcgtc
     1621 ctattacctc aatcccatca atatctggta gtcctaaatc caaaattatc aaatctgggt
     1681 caagagaatg tataagtgat atagctgttc ctccattatc tgtctctaca cacctataat
     1741 tttgagtttc aagagatact tttataaatt tacaaattgg cttatcatct tctacaatta
     1801 gaactaatgg ctttatttcc acttgtcttt cccctcctca tcatacatag gtatgctaaa
     1861 tctaaatgta gctcctccag aaggattatt aaaggcgaca atttctccac catgagcttc
     1921 tactatagat ttacatatct ccaatccaag accaatacct cttctttcaa gatttctaga
     1981 cttattctta gtataaaacc tattaaaaat attcccgata tcttcatttg gtattccttt
     2041 tccattgtct gcaacttcaa atagtacttg atttgactct ttttttacag ttagttctat
     2101 cttagaattt ttaggtgtat gccttatagc attatcaatt agatttacta acacttgttc
     2161 tattaataat acatccacat gaacgattat tatctcatcc ggaatatttg tttttatgtc
     2221 tctactattg gcaaactttt tgactcttaa tatagattca gctattattt catcaacaac
     2281 ctctggtcgt ttttcaatat ccaattttcc ttcatctatt ctggtcatac taagtatatt
     2341 ctcaacagaa tgagttagcc aactcgtatc ttcatatata tttttcaata attctactct
     2401 agtttcttta tctatcacat catcattttc aagtattgtt gaagatgaac ccaatatact
     2461 tgtaagtgga gttcttaaat catgagaaat tgacctaagc aagtttcctc ttaacctctc
     2521 tctctctgcc tcaagatttg cttttttatt tttctcaaat aaattttctc tatcaattgc
     2581 caacgcaacc tgtgttgaaa ctgcttctaa tagtatcttt tcattttctg taataatatc
     2641 tatcttaaaa cacgcaacac ctataacacc aagaacattt gattcttgac cttttatagg
     2701 gaaataatat gcactgctgt ttttacaata ttttgtccca attccaactg ggttacctgt
     2761 cctaaaagct tcttttatag cttccttttc taatactgaa tggaatatat tctcacttcc
     2821 atcattgtta caaatataaa tacttggctt acctagatta ttattctcat ctgcaatagt
     2881 cactattaca gttctattta atatatttac taaattagac ccacaaaatt caataatttg
     2941 atttttattt tttgctttaa gcaacgtttt gttattctca tagagcaatc ttatcatttt
     3001 ttctctctta tgagaaagtc tcacttctct ttttatccta gaagttaaag tacttgttat
     3061 aattgctgcc attagcataa tacaaaaggt cataggataa tcttttctat aagctaaaaa
     3121 agtatataat ggatgcgtaa agaaaaaatt aaatgatagc actcctatta ctgaagaaag
     3181 tattccaaaa aaatatccat aggttatact tgtagcaaat agtacaccta atatgtatac
     3241 tagtataata ttagattcat taaaacctac cttttcaaaa actactgata ttccagtagc
     3301 aattgtcata accaatatag tttttatgaa gtccttaata attatctttt tactgagata
     3361 gccatcttca tcataatcat actcttttaa gtcttcatgt tgtagtcgtt cctttttcat
     3421 ttactacaat tcctccttat tgttactgtt taaattggct ctattttata taatacaatt
     3481 ttatcataat aaattcaatc aatacattta attaatgtac ttaaattact aaaaggtgct
     3541 gtcttaaaat gaaaataaat ctctatttca aaacagcacc tatatattta aattactaat
     3601 ttagttttta catacttaaa tcaatagttt tattcaattt cataaattat aatacttcaa
     3661 ttataaaaca tttctatcca acaagcataa atataaagtg ggcacatata cctgcacata
     3721 aaccacctat agtatgcgaa agtatcgctt taccagccaa tggtcttgca tctaaagcat
     3781 ccatcatacc tatgtgtgta cttaaatatc cactccaaca catacccatt gctgtgaata
     3841 ctgctatatc attaggagtt atagcgcctg attttataaa ctctggtact aaagatattg
     3901 ctgctccaac agctcctaat gcagttacag ggaatgctat tgcctctggt gatgtgaatc
     3961 caaataatgg ttctattatg aagctaatct tatcaccaat agcaggtaat aattttatac
     4021 cttcatatgc agctcctgtg taaacagctt gacctgtagc agggtctgta gatggtccaa
     4081 aagttaataa cataactaat gtacaaacta ctaaaactcc tggtattata gccataccca
     4141 tttcaacacc taacttacca ccttcaagta tagcatccaa tgttctttgg aatacattac
     4201 catctcttat ttctctaaac tcttcaagtt ttgcaccttt atcatttatt tgcttttcag
     4261 tatcattttt agggtcataa ttataatact ttttagtaaa agtcaacata attctaacac
     4321 taataatact acctattatt gccgcaacat tacctattat agctggtagt acatattctt
     4381 taccttgagc tatcataaaa gttgttacaa taagtcccat accaaaggct gttcctaaat
     4441 tacataaagc aggtacttga tacttcttaa aatattgtgt aaatgttttg tctttagcaa
     4501 atggtataat agctggatta tcagataaat atgttgcaac tactcctgct atactagcac
     4561 caggtaagcc ccatataggt ctcataagac ccttaaatat cttattaact aaagatatta
     4621 ctccaaactc tgaaagcaat gcactaagtg cacctgcaag aactgccata gccattatta
     4681 aaaatactgt gtctaataaa agcgcatgtg ctgtactcat tattacttta aacatcattc
     4741 ctgcacccat gatgcttcct acatatccaa atcctaccgc caatagtaca ataaatacga
     4801 aggtttccat acttactgcc ttgactcttc tcttatccga cagcttttcc atcttgaaat
     4861 cttccccctt ttaattttaa attaaggttc ctaacattgg tacaaatttt ttggtacaaa
     4921 ctgagttcta aaacttacac tcgtaatgca agttgtcagt tttttgaaat aaaaaaaaat
     4981 tacgtttata gaataagaat atcctcttta atttggatac tcttattcta ttttatgtta
     5041 ttatattatc atccttttgt ttaaaagtca attttttcta tttttgttgt ttaattttat
     5101 ttatactctt tttatatttt tcattttctt ttatctttgt aagtactcca tataagttga
     5161 cttttctaaa tacagtagtt ataaatacca atcctaatgc aattgaactt gcacacgata
     5221 atgtactcac cccatattct acagctttta tataattatt ggtcaaaaca gaatacatag
     5281 tattatatat tccataacct ggtactaata ttattaaaca gcaaacactt aatgtagttg
     5341 ctggagtcat ataaattctt gcacataact cacaatatat cgcaaaacaa actgtagcta
     5401 taaaaaaaca taagttttct gttactccca tatttaaaag aaatttatac gaaaaccaag
     5461 ctatacttcc acctattcct gctagtatta agttttttcc tttcatatta aatagcactc
     5521 caaagcttaa ggcagtaaaa aaagctgcta ttacttctat aaacaaatgc acttatatcc
     5581 cccctaactt gaagtacaat ccaagtacca agaacattcc tactgctata gatacaccta
     5641 caaaaaatac ttctgctaat tttgcaagac cagaaatcaa ctgtccatct aataaatctc
     5701 ttaatgcatt agtcaaagca agcccgggaa ctaagagcat tattgctcct gctattaaat
     5761 ggtctaacgt ctttatgaat ccgagcttta aaattaaaat tgaacaagaa gctataataa
     5821 ctgctccaat tgcatttaca aaaaagtcat taagggaaga tttacttaaa tacataataa
     5881 caaacttagt cagaatccca acagtaaatg cagctataaa ttcttccaca tttcctccaa
     5941 ataatatcga aaaagttgct gctgcaatac ctgcaaaaaa tattgttatt aaatccgagt
     6001 aatagttatc ttcacttatt tcatctaaaa ttttctcaca taattcaata tctaaattat
     6061 ttatacttat tgctcttgat aatgagttaa cttcatgaac caagtttagg tctactcttc
     6121 ttgagcttat tcttttaatt gctgaatgta atttcccatc tacaaataca gatgccatta
     6181 cagctgttgg agttgcaaat acatctacgt gatctattcc aaaagattga catattctag
     6241 ctatcgtttc ttcaactcta taggtttctc ctccactttg caacatagct ttacctgcat
     6301 ttgaggaaaa gtttaaaatt ctatctatat ccacaaaaca tatctcccat tcttttatag
     6361 tattattcat attttaatac aagatattat ttatttaaac tattttaaat aattcatatc
     6421 caatatttac attttatgaa aaaacttgtt ttttatgctt gtcaaaatca aattttaagc
     6481 ttgtttacac cataaaatgg ctgaattaat atttgacaaa cactttcaac catttatata
     6541 accttctaaa ttgtataact ttttactaca aataaaatta tatctctttt aattctttct
     6601 cttgagtatt tagcttatag taacttcata tttatatatg tacaatataa aacattattt
     6661 ttatgataca aaatataatt tgtaaatgta tatatgtatt aactgtgtat atataaatta
     6721 ttcttaacct tatcagtttt ttcatatatc ttcatctata ccgaccatat attaataaat
     6781 gatattacat agacttaagt acattaaaaa acttaaattt agttatttaa caacataata
     6841 ctctaattta atctataaaa atccatacct aatataatgc aactcttatt ttaacaccat
     6901 ctattacaca aaacaacata ttaaaaagta aaatacaaaa tatccactat aaaccttatt
     6961 ttaatactga atatatatta taatatattt aatgtagctt aaatatagtt taaacataaa
     7021 gaaaggaaac ggtatatgct aagaaaagat aaagttctat tatctctaca taaattatgt
     7081 gaaaatatga ctttaaaaga ccttcaatca gataaaacag gattcactac aatagaaatt
     7141 gctaaccatt catctcttga tagaagtaat accagtaaag aattaaataa cctatacaat
     7201 gaaaaaaaag ttctgaaaat atctggtaag ccaattttat ttctacatgc tagtattata
     7261 gaaaatttaa ttggccgtac tttgaacagt gatgaatttt ctataaataa ttgtaatgag
     7321 ctacttaaat caagtaaaaa tgactcagaa gatatttttt caacattact tggacatgat
     7381 tctagtctta aactacctat agaaaaagct aaatctgcta tactctatcc tccaaaaggt
     7441 cttaatacgt tactaatagg acctactggt gttggaaaaa gtacatttgc tgaaacaatg
     7501 tacaaatatg ctgtagaaag taaaatcttt tcttctgaaa gcaaattcgt agtatttaac
     7561 tgtgctgaat atgcagaaaa tgctaactta ttattaagta atttatttgg ttatgtcaaa
     7621 ggttctttta ctggtgctaa taaggataaa ttcggcatag ttgcacaggc tgatggaggc
     7681 atattattct tagatgaaat acacagatta ccaccagaag gtcaagagat gctttttctt
     7741 ttgatggaca aaggcatata cagacgatta ggagaatctg acattgtgca taaagctaat
     7801 gtctttattg tgtgtgccac aacagaagat atacattctt ctctgcttgg gactttttta
     7861 agaagaatcc ctgttacaat caacctacct gctttacagg ataggtcttt aaaagaaaga
     7921 ttatctctta ttaaatactt ctttattata gaatcaaaat acaccaatgc aactataaaa
     7981 gttcacaaag atgtaattaa atcattactc ctttatgatt gttttggtaa tataggtcag
     8041 cttaaatctg acatacaact tatgagtgca agagcattct taaattacaa aactggactt
     8101 aaggataata tagaaataga tttatccatt gttcctgatt acatctatat tggtctttta
     8161 aaattgaatg aaaatcgtag taatctaaat aaattatcag aacttgatta tatgcttttt
     8221 tatgaattta atggtaataa cttagaagtt gaatatacaa aagaactatc taaatacaat
     8281 atagatacat caaacaatgg atttaaatac tctacagaga ttccttatct ggaaaatgat
     8341 ataaggagta tgtttataaa ttacatggaa aagtactcta caaaattact ttcgatatct
     8401 tcagtaactc attctgatta tacaagtaat gagattttta aagtaatcaa tccaaagatt
     8461 tactatgctg ttgaacattc tttaaaagag gctgaaaaaa tatcgagaaa caaatacaca
     8521 aaacaaactt atattgctct ttcaatgcat ataagtgcac ttatagaaaa tataaatcaa
     8581 gacacatata atcctaagta caatttatat aatgataaaa ttttcttaga taaagattta
     8641 aatatagcta aaatagtttt aagtataatt gaaaatgact tacaaacatc tttacctaaa
     8701 gatgaagtaa aatttatatc catgtttcta aatagcatgt atcttgaacc aaacaatcaa
     8761 tctgaaagta taggagtcat aatacttgct catggaaatt caacagcaag cagtatgtgt
     8821 gatgtagcaa attcattgct tggtacaaac cattgtaatg caattgacat gcctcttgat
     8881 gtaaaagtaa gtaccatact tgataaaact atagagttgg taaaagaatg tgacgaaggt
     8941 aagggtgtac ttcttttaac agatatgggc tctcttatag cttttggaga acttatttca
     9001 gaaaaaacaa aaatcaaaac tcgctcaatt gaaatggtat ctactccaat agttttagaa
     9061 gcagtaagaa aaagtacact accagaaatg actttggatg aattagctaa ttccttaagc
     9121 tctttaaatc catatattgg acgagcagca atcgattcaa cagaaataaa ctatcctgaa
     9181 aagtcaagag agtatgtaat aataactact tgtataacag gatatggttc agctatgaaa
     9241 gtagcagact ttataaattc ttcacttaaa aatattagta aatacaatat taatttagtt
     9301 cctcataata tagaaagttt taaaatgtat aagaataaat ttagtaatac taaaattctg
     9361 gcagttgttg gaagtgtaga ttttaatgta gccaacaccc cttttataca catagaggaa
     9421 ctactaaatg gatatggcct agatatgcta aacaaaataa tagaaggtac attcaatgcc
     9481 aatagtatta caaatataaa agaaacaaat ctatcataca acagtttaat aataaacttt
     9541 atgacccaaa acttagagct tttggactct gttaaattat tcaactatgt caattcttcc
     9601 ttatttacaa taatagaatt aatagatgaa aacttaaaga gcaaatttaa gctaggatat
     9661 atactacatt gctcttttat gatagaaaga tgttttaaaa atacaacttt tccatataat
     9721 aacattactg aattaattag taaaaatcat gaactatata gtttaatacg tgaagccttt
     9781 aagccaacag aagcttattt taatattttg ataccagacg aagaaattgc ttatataata
     9841 gacatgatta atgagtatct aaataaataa aatttatagc acttattttg tgcatattaa
     9901 ttaatatatg aaatggagga cgagattttg aaaggaaaaa taaaacagtt aacaatactt
     9961 gcattaattt ttatatttat aactcctgta tttgcatttg cagatactcc accagtacca
    10021 aattcttcaa gagcagcact acttatagac caggaaacta aaagaatatt atttgaaaaa
    10081 aacattgatg aaaaaatgcc attagcgagt ttaagtaaaa tgatgacctt tttattggca
    10141 atagaagcag ttgataaaaa tcaagttaaa gagactgata tggtgaaaat agacaagtct
    10201 acagcttcag taggaggttc tacttgtaaa cttaaagatg gtgatgaaat atctctagga
    10261 gagcttatgc agggtctaat gctagtatct ggaaatgatg ctgctatagc catagctaaa
    10321 catattggta aaactgaaaa gaattttgta aatatgatga acaagaaagc agaagaaata
    10381 ggaatgattg atacgtatta ttttaatcca aatgggcttc ctatatatac tgacccagaa
    10441 cataaagaac ctcctattga aaatatgtca actgctcacg atatagttac acttggaaag
    10501 tatatgtacg accattatga aaatcaagtt acaagaataa ccactatgca agtatataat
    10561 gatactaaaa aggattttac acattataac acaaatcctc tgcttgtttc agtacctggt
    10621 gttgatggaa taaagacagg atatacagat aatgctggat attgccttgc attttctatg
    10681 atggtaccta aagatgctaa aaacgaaaga aatcatagat tgataggtgt tgtacttggt
    10741 gatggtaaca agaaaaatag aatttcttct tccgctactt tattgaaata tggtaaagat
    10801 aatttccatt ctaaaaagat agcacataag ggtgatatta tagagactcc ttgtgttgat
    10861 ggtattgatg attttaagat aacagttaag gttgataaag atttgtatgg tgttgtttca
    10921 gataacgaga atatcaatcc taaggtagtg tttaaaaata tgaattatcc tatccataag
    10981 ggagatattg ttggtgttgc aaagtattat aatgactctg gtaaatttgt tggaagtgtt
    11041 gatgtaaaaa gtgaaagtaa tataggatgt attcctttga aggataagat taagattaag
    11101 gttgctaaga taaataaaaa acttgagatt aaaaattctg tttgttttaa agcatagtaa
    11161 agcatattat taaaagggta tgttaaattt ataaagactc cactttgtgg agtctttttg
    11221 ttttttacaa aaaattaaaa tagtggcaac aattgaggat gtgagggaga gttgccacta
//
LOCUS       DAELLV010000120        11004 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.120, whole genome shotgun sequence.
ACCESSION   DAELLV010000120 DAELLV010000000
VERSION     DAELLV010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11004
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.120"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1701
                     /locus_tag="KPD58_002353"
     CDS             <1..1701
                     /locus_tag="KPD58_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBG0266115.1"
                     /translation="FRLFKCYNTLWRYAGEEEIISIFVATLAYLIPIYIINKLLGFDY
                     PIMFYVLNTIFIIMFTSGARIAYRAIRIVMNKTYSRGKVSNILIIGAGDAGEMVIQEL
                     KRNPELKKVAVAIIDDDKNKIGRIIHNVKIVGTTSDIKDVVEKYNVDEIIFSIANIEK
                     RRKKDIIDICKNTNCKIKTIPGIYEIIDGKVDIKQIREVEIEDLLGREPIKTNLREIS
                     NYIEGKVILITGGGGSIGSELCRQIAGFNPKELIIVDNYENNAYSIQQELLRKYKNKL
                     DLKTVIASIREEKRMDEIFNKYKPEVVFHAAAHKHVPLMESSPGEAIKNNIFGTLNIA
                     GLSSKYRAKKFVLISTDKAVNPTNIMGATKRAAEMIIQTMNAESQTEFVAVRFGNVLG
                     SNGSVIPLFKKQIEDGGPVTVTHPDIIRYFMTIPEAVGLVIQAGAMAKGGEIFVLDMG
                     EPVRILDLAKNLIKFSGFEPDVDIKIEFSGLRPGEKLYEELLMSEEGLLDTEHKKIFI
                     GRPIDVDREKITKYLKLLREITNNEEVEKIDGIMRELVPTYIKPEDANIKEIATTREK
                     "
     gene            1900..2607
                     /locus_tag="KPD58_002354"
     CDS             1900..2607
                     /locus_tag="KPD58_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG0266116.1"
                     /translation="MMNIKNKKHILKKFIAMVLIAGVVTVEAGAITASAAEPTNSPMS
                     ATVDQCDFLNVRSGASANDAVVGKINTGDKVEVLELHSNGWIKIKSVDNVTGWVNGDY
                     LTIQGGNVDAKVQNVLNLAFKQQGKPYKWGATGPNSFDCSGFTSYVYKNGAGVNLPRV
                     SRSQATVGKKVSRAELKPGDLVFFGSGGSINHVGLYVGDSKFIHSPQTGDVVKVTSMA
                     PGTNYAKRLITATRVLQ"
     gene            2909..5020
                     /locus_tag="KPD58_002355"
     CDS             2909..5020
                     /locus_tag="KPD58_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding glycosyl-hydrolase Cwp19"
                     /protein_id="HBG0266117.1"
                     /translation="MKKISILVLSLIMTLTMCSVSSFADSSNDKEMRAAWISTVYNLD
                     WPKTKNNEAKQKKEYTDLLDKLKSVGINTAVVQVRPKSDALYKSNINPWSEYLTGTQG
                     KDPGYDPLPFLIEEAHKRGMEFHAWFNPYRITMADESIDKLPANHPAKKNPSWVVKHG
                     NKYYYDPGLPEVRKYIVDSIAEVVQNYDIDGVHFDDYFYPGVSFNDTATYQKYGKGQN
                     KDDWRRENVNTLLRDVKASIKSIKPNVVFGVSPAGIWRNKSSDPTGSDTSGNESYVGT
                     YADTRAWIKQGLIDYVVPQLYWPIGLKAADYSKLVAWWANEVKGTNVDLYIGQGIYKQ
                     GQSSYGGQNIAKEIVQQVTLNRKYSEIKGSMYFSAKDIANSTSIQKDLKSLYSSSEEP
                     VTPPSNVKVEKLRGDERYDTAVAISKKGWATNSDTVVLVNGYSIVDGITSTPLATSND
                     APILLVNKDNIPTSTKNELKRLNPSKVILIGGNNSIGDKVESEIKDTLSNVSINRVGG
                     SDRYSTSLMIAKELVKTNPVEKLYITSGTGEADSLSIASKAGEEKQPIVLVSKDNVSD
                     EVYNWISDLKVKDAYFIGGNLSISDSVINKLDKVITNDVSKNRIAGENREETNGKVIQ
                     KFYPNAEYSSMFVSKSNQLVDALTSGPLAAKLKSPVVMLGNSVTSAQKTALEPKKTTL
                     VYEAGDGINQNTLNTFLNLVK"
     gene            complement(5218..6183)
                     /locus_tag="KPD58_002356"
     CDS             complement(5218..6183)
                     /locus_tag="KPD58_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBG0266118.1"
                     /translation="MSKLKKFVILLAFLVVIFPISVYGYFYYKLSAIHDSSISSDLLD
                     NNDHKNEDGIINILLMGTDARPNEDSSRSDAMMILTIDNKHNDIKLTSLARDSYVDIP
                     GHGKQKLTHAYAYGQADLLIQTIEENFNIDIQNYACVNFESFMYIIDAIGGVEVTVEK
                     GEIRELNKFIPETYKWNKSDDKGSIQYIRNSGKQTLNGYQALSFARIRHNDTAFARDG
                     RQRQIIQAIIKKTETLPVTKYPGLLDAVLPYVKTNMKPNAILSLGAQVLKMGDLNIKQ
                     FEFPIDDEIHSTGGIYGKAGWVLRFDPDTLDILHDFIFNDIEFKQ"
     gene            complement(6468..7424)
                     /locus_tag="KPD58_002357"
     CDS             complement(6468..7424)
                     /locus_tag="KPD58_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBG0266119.1"
                     /translation="MSGLKKFVIFLALLVIAFPVSIYGYVFTKLKTIQDDSINTEALS
                     SNDYKNEENITNILLLGTDARPGEEVSRSDAMMILTIDNKNKSLKLTSLNRDTYVNIP
                     GRGEQKLTHAYVYGQANLLIETIEENFQLDIQNYAVVDFFSFMDIVDALGGVTVTVDK
                     SEIRELNKFIPETYKWNKNDNKGSLKYIRNSGEQKLNGYQTLAFSRIRKNDSTDERDR
                     RQRSVIQSLINGVKDLPVTKYPNLVNTILPYVKTNMNPNEIISLGKELLSIGNLNLKS
                     MEFPLSTENGRKIGNAGYVIPFEEYELDAMHDFIFKDIMVED"
     gene            7792..8580
                     /locus_tag="KPD58_002358"
     CDS             7792..8580
                     /locus_tag="KPD58_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG0266120.1"
                     /translation="MIKLIATDLDGTLLDEKSEINPEFYKVFKKLRERGIMFSAASGR
                     QYQNLIKKFEDIKDDMMFISENGTLVVYKGKEILSNPLNKELVNEIIETTRSIKGKKI
                     VMSGKKYAYIESKDEAFIQEVSTYYAKFKIVEDLTKVEGDILKIAVFDFKGAEHNNNI
                     YFEKFSDRAQVCISGVEWLDLTAKGANKGSAIKKVQKMLDIKYEETMVFGDQLNDVEM
                     MKSAYHSYAMENANEHLKQIARFRAKRNTENGVVDKIKEVIKIG"
     gene            complement(8703..9173)
                     /locus_tag="KPD58_002359"
     CDS             complement(8703..9173)
                     /locus_tag="KPD58_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HBG0266121.1"
                     /translation="MKKILILFFSIILSLGMVSCSSSGDYKNVSTSKIKKALKSSDLL
                     IKESKIFDAKDFNYFNDVEEDIIQGFVINSSEKLSLEDIIVIKTDEVDKIYKTLENYK
                     KDMIQAPFGEGHGEKINSEIANNTIIEKAGRYVYLISAENATQIENKILNIIKK"
     gene            9312..9956
                     /gene="uppS"
                     /locus_tag="KPD58_002360"
     CDS             9312..9956
                     /gene="uppS"
                     /locus_tag="KPD58_002360"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HBG0266122.1"
                     /translation="MRIPKHVGIIPDGNRRWASQNGMTKDKGYESGIDPGLESYRLCR
                     DLGCEEVTFYGFTTDNTRRPSVQTKAFTKACIDAANILIDEGAALLVVGNTKSPMFPE
                     ELLPYTNRIKAKEGQIKANLLVNYGWYWDLNNISGVENVNKNNIQDYIQSFDVSRLDL
                     IIRWGGRRRLSGFLPVQSIYADFYVIDNYWPDFKSDDIKDAFSWYDTQDVTLGG"
     gene            9999..10904
                     /locus_tag="KPD58_002361"
     CDS             9999..10904
                     /locus_tag="KPD58_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG0266123.1"
                     /translation="MKKKLLDGKITLIICKSVKIYTKKGEEMSKGNNNNNSRNKSKKT
                     SHLNRKKRKLNKKKLAVLICFTVLFLFIAFKATQGVVALVKSMDKSNKTNQQQNVNSE
                     QFDLGNEEENKKKKYTVFIDPGHGGNDKGTESKTSNRYEKDLNLQIAKKLANKLSKQK
                     DIQVVVSRTDDTYISLKDRAILANNSSADVLVSIHLNAEKNGNTATGIETWYRNKATD
                     GSKELAQTVQSTIVSYVKVRDRGIVENNFEVLRESNMPAILIECGFLTTPSEEQKIIN
                     EKYQDQLAEGIVQGVLSYLDSKGNK"
BASE COUNT         4347 a         1299 c         1849 g         3509 t
ORIGIN      
        1 tttagattgt ttaagtgcta caacacatta tggagatatg caggagaaga agaaattata
       61 tctatttttg tggcaacttt agcttattta attccgattt atataataaa taaattatta
      121 gggtttgatt atcctataat gttttatgta ttaaatacaa tatttattat aatgtttaca
      181 agtggcgcaa gaatagccta tagagctata agaatagtca tgaacaagac atattctaga
      241 ggaaaggttt caaatatcct tataattggt gcaggagatg caggagaaat ggttatacag
      301 gaactaaaaa gaaatccaga acttaaaaaa gtagcagtgg ctataattga tgatgataaa
      361 aataaaattg gaagaataat ccataatgtg aaaattgtag gaacaacttc agatataaaa
      421 gatgtggtag aaaagtacaa tgtagatgag ataatctttt caatagcaaa catagagaaa
      481 cgtagaaaaa aagatattat tgatatatgt aaaaacacta actgtaaaat aaagacaata
      541 ccaggaattt acgaaattat agatggaaaa gttgatataa aacagattag agaagtagag
      601 atagaagact tgttgggtag agagcctata aaaacaaatt taagagagat aagtaattac
      661 attgagggaa aagtaatcct aataactgga ggaggaggct caattggttc agagctttgt
      721 agacagattg caggatttaa tcctaaagag cttattatag ttgataatta tgaaaataat
      781 gcttactcaa tacaacaaga attacttaga aaatataaaa ataaattaga tttgaagaca
      841 gtaatagcat caataagaga agaaaaaaga atggatgaaa tatttaataa atataaacca
      901 gaagtggtat ttcatgcagc agcacataaa catgttcctc ttatggaatc gagccctggc
      961 gaagctataa agaataatat atttggaact ttaaacatag ctggcttgtc ttcaaaatat
     1021 agagctaaga aatttgtcct tatatctaca gataaggctg taaaccctac taacataatg
     1081 ggagctacta aaagagctgc tgaaatgatt atacagacta tgaatgctga aagccagact
     1141 gaatttgttg ctgttagatt tggaaatgtg cttggtagta atggtagtgt aattccactt
     1201 tttaagaagc aaattgaaga tggcggacca gtgacagtta ctcatccaga tattattaga
     1261 tattttatga caataccaga agcagtaggc ttagttatac aagctggggc tatggctaaa
     1321 ggtggagaaa tatttgtgct tgatatggga gaacctgtaa gaatattgga tttagcaaaa
     1381 aacttaatta aattcagtgg atttgagcca gatgttgata taaaaattga attttctggt
     1441 cttagaccag gagaaaagtt gtatgaagaa ctacttatgt cagaagaagg tctattggat
     1501 acagagcata aaaagatttt tattggtaga ccaatagatg ttgatagaga aaaaataaca
     1561 aaatacttaa aacttttaag agaaattact aacaatgaag aggttgaaaa aatagatggt
     1621 attatgaggg aattagtacc aacttatatt aaaccagaag atgctaatat caaagaaatt
     1681 gcaacaacta gagaaaagta gaatatattt ctaatttgta accaggggga gcaaaaagaa
     1741 acaaatatat actaaaaaga aacaaaagat tacaaaaaga ttacaaaaag attacaaaaa
     1801 aggttgcaat ttattttgat gtaagataga atgttaatta ggaattggaa acgaaggaac
     1861 aaaatgttaa attttcgaaa ggattaggta tttgagatta tgatgaatat taaaaataaa
     1921 aaacatatat tgaaaaaatt tatagctatg gtgttaatag caggagttgt cacagtagaa
     1981 gcaggagcaa taacggctag tgcagcagag ccaactaatt cacctatgag tgcaacagta
     2041 gaccagtgtg attttttaaa tgtaagaagt ggagctagtg caaatgatgc agtagtagga
     2101 aagataaaca caggagacaa agttgaagtt ttagaattac attcaaatgg atggattaaa
     2161 ataaaaagtg ttgacaatgt tacaggatgg gtaaatggag attatttaac tattcaaggt
     2221 ggaaatgtag atgcaaaggt acaaaatgtt ttaaacttag catttaagca acaaggtaag
     2281 ccatataaat ggggagcaac tggaccaaat tcttttgatt gttctgggtt cacttcttat
     2341 gtctataaaa atggagcagg agttaattta ccaagagtat caagatcaca agcaacagta
     2401 ggaaagaaag tatcaagagc agaattaaaa ccaggagatt tagttttctt tggttcaggt
     2461 ggttcaataa atcacgttgg attgtatgta ggagattcta aattcataca ttcaccacaa
     2521 acaggagatg tagtaaaagt tactagcatg gctccaggaa ctaattatgc aaaaagactt
     2581 ataactgcta cacgtgtttt acaataataa tagatattga tttacatagg attgaatatt
     2641 aacattaaat atataattac atttaatgtt ttacatagag atttaagagt ttagtaatat
     2701 taaaatagtg atgaacgtta taaaaacagc aaagaagata aatttctttg ctgttttttt
     2761 tataataaat tatttcatat tataatcaaa tatcaaatat aaattttaac tatattattc
     2821 aatatttgac agataatatg aattttagat atacttaata agaagtatat tgtagaaaaa
     2881 ataaatttaa aaaattaagg gggatgaaat gaaaaaaata agtatattag tattatcatt
     2941 gataatgaca cttactatgt gtagtgtaag ttcatttgct gattcatcaa atgataagga
     3001 aatgagagct gcttggatat ctacagttta taatttagac tggcctaaga ctaagaataa
     3061 tgaagccaag caaaaaaagg aatacacaga tttattggac aaattaaaaa gtgtaggaat
     3121 aaatacagca gtggttcaag taagaccaaa atcagatgct ttatataaat caaatataaa
     3181 cccatggtct gaatatctta ccggtacaca aggtaaagac cctggatatg acccattacc
     3241 atttttaata gaagaagcac ataaaagagg aatggaattt catgcatggt ttaatccata
     3301 tagaataact atggcagatg agagcataga taaattgcca gcaaaccatc cagcaaagaa
     3361 aaatccaagc tgggtagtta aacatggaaa taagtattac tacgacccag gtttaccaga
     3421 agttagaaag tacatagtgg atagtatagc agaagtagtt caaaattacg atatagatgg
     3481 agttcatttt gatgattact tctatccagg agtaagtttc aatgatactg caacatacca
     3541 aaaatatgga aaaggacaaa ataaggatga ttggagaaga gaaaatgtaa atactctact
     3601 tagagatgta aaagcgtcta taaagagtat aaagcctaat gttgtatttg gtgtaagccc
     3661 agcaggtata tggagaaata agagtagtga cccaacaggt tcagatacat caggtaatga
     3721 aagttatgta ggtacatatg cagatacaag agcatggata aaacaaggat tgattgacta
     3781 tgtagttcca caactttact ggccaattgg tcttaaagca gcagattatt ctaaattagt
     3841 ggcatggtgg gctaatgaag taaaaggaac aaatgtagac ttatacatag gacaaggtat
     3901 atataagcaa ggtcaatcga gttatggtgg acaaaatata gcaaaagaaa tagtacaaca
     3961 agtaacatta aatagaaagt atagtgaaat aaaaggtagt atgtatttct cagctaaaga
     4021 tatagcaaat agtacttcta tacaaaagga tttaaagagt ttatattcta gtagtgaaga
     4081 accagttact ccaccatcta atgttaaagt ggagaaatta agaggcgacg aaagatatga
     4141 cacagcagta gcaataagta aaaaaggttg ggctacaaat tcagatacag tagtattagt
     4201 aaatgggtac tctatagttg atggtataac atcaacacca cttgctacat caaatgatgc
     4261 tcctatacta ttggtaaata aggataatat acctacatcc actaagaatg agttaaaaag
     4321 acttaatcca agtaaagtta ttttaattgg aggaaataac tctattggag ataaagttga
     4381 gagtgagata aaagatacat tgtcaaatgt atcaataaat agagttggtg gttcagatag
     4441 atatagtaca tctttaatga tagcaaaaga attagtaaaa actaatccag tagaaaaact
     4501 atatataaca agtggtacag gtgaagcaga ttctctttct atagcttcaa aagcaggaga
     4561 agaaaagcaa ccaatagtat tagtatcaaa agataatgta agtgatgaag tttacaattg
     4621 gatatcagat ttaaaagtaa aagatgctta ttttataggt ggtaatctaa gtatatctga
     4681 ttcagtaata aataagctgg ataaggtcat tacaaatgat gtatctaaaa atagaatagc
     4741 tggagaaaat agagaagaaa caaatggaaa agtaatacaa aaattctatc caaatgctga
     4801 atattcatca atgtttgtaa gtaaaagtaa tcaattggta gatgcattaa catcaggtcc
     4861 tttagcagct aaattaaaat caccagtagt tatgcttgga aatagtgtaa catctgctca
     4921 gaaaactgct ttagagccta agaaaactac tctagtatac gaagctggag atggtataaa
     4981 tcaaaacaca ttaaacactt tcttaaactt agttaagtaa taaaaaagtt aatttaaaat
     5041 acagagtatt ttataaatgt agattaaaat gaaaattatc atgataaatt agaaaataaa
     5101 aaagaactat atgcattttg taatcaagtg tacacagcaa cttgaaaata attatgcgat
     5161 atagttcttt tttagtaatt aatcttatac tttttaataa actttatgtt gaatttttta
     5221 ttgtttaaac tctatgtcat taaatataaa atcgtgtaat atgtctaaag tatctgggtc
     5281 aaatctaaga acccatccag cttttccata tataccacct gtactatgaa tttcatcatc
     5341 aataggaaat tcaaattgct taatatttaa gtcgcccatt tttaaaactt gagctcctaa
     5401 agaaagtatt gcattaggct tcatgttagt tttaacatat ggaagaactg catctaataa
     5461 acctggatat ttagtaacag gtaaagtttc cgttttcttg attatagctt gtataatctg
     5521 tctctgtctg ccatctctag caaaggctgt atcgttgtgt ctgatacgtg caaaagatag
     5581 tgcctgatat ccatttagag tctgctttcc agagtttcta atgtattgta tgctaccttt
     5641 atcatcactt ttattccatt tataagtttc aggaataaat ttatttagtt ctcgtatttc
     5701 tcctttttca acagtaacct caacgccacc aatagcatca attatataca taaatgattc
     5761 gaaattaaca caagcataat tttgaatatc aatattaaaa ttctcttcaa ttgtttgtat
     5821 taataaatca gcttgtccat aagcataagc atgagtaagt ttttgtttac catgaccagg
     5881 gatgtctaca tagctatcac gagctaatga tgtaagtttg atatcattgt gtttgttatc
     5941 aatagtaagt atcatcatag catctgacct tgaagaatct tcatttggtc tagcatctgt
     6001 tcccataagt agtatgttaa ttatcccatc ttcattttta tggtcattgt tatccaataa
     6061 atcactactt atgctcgagt catgaatagc agataattta taataaaaat atccatagac
     6121 acttataggg aatattacta ccaagaaagc cagtagtata acaaatttct ttaattttga
     6181 caaaagtacc ccttctttct tctttttcct gtatattttt taatagcata cacttaatta
     6241 tattaaagta ttgagatata ttcaatactt gtttcagtta caaaaaaaag acaatgatat
     6301 aatttataag aaaatttatc gacctatata tatagttaaa agtatatata atatattttt
     6361 taaaagtaat ttatatttta aaataaaaac acaccccata aatacaaagt aatttgcatt
     6421 taagaggtat gtatttatca tcaaattatt ggtttgtttt ggaatttcta atcttcaacc
     6481 ataatatctt taaatatgaa atcatgcatt gcatctagtt catattcctc aaaaggtatt
     6541 acatagcctg cattacctat ttttctacca ttttcagtag aaagaggaaa ctccatagat
     6601 ttcaaattta aatttcctat acttaaaagt tcttttccta aagatattat ttcattagga
     6661 ttcatattag tttttacata cggaagtatt gtgttaacta gattaggata tttagtgact
     6721 ggtaaatcct taacaccatt tattaaactc tgtataacac ttctttgtct tctatctctt
     6781 tcatctgtac tatcattttt tcttatacga gaaaatgcaa gtgtctgata tccatttaat
     6841 ttttgctcac cagaatttct tatatattta agagaacctt tattgtcatt tttattccat
     6901 ttataagttt caggaataaa tttatttaat tctcttattt cactcttatc aacagtaaca
     6961 gtaactccac ctaaagcatc aactatatcc ataaaagaga aaaagtcaac tacggcgtag
     7021 ttttgtatat caagttggaa gttttcttct atagtttcta ttaataagtt agcttgtcca
     7081 tatacgtagg catgtgttaa tttttgttct ccacgtcctg gtatattaac ataagtatct
     7141 ctatttaatg aagttagctt aagactttta tttttattat caatagtaag tatcatcatg
     7201 gcatcagaac gagaaacttc ttctcctggt cttgcatctg tacctaacaa taatatgttt
     7261 gtgatatttt cttcgttttt atagtcatta ctactaagcg cctctgtatt tatactatca
     7321 tcttgtatag tttttaattt agtaaaaaca tatccatata tactaacagg aaatgctata
     7381 actaaaaggg ctagaaatat aacgaacttt ttgagtcctg acaataatat cccctacttt
     7441 ctaaattttt taattacaca tagttaatta tattaaaaat ttgttacata agcaataaaa
     7501 aacacaataa attagcataa gtttgtcata atataattat ttttatagca ttttatagaa
     7561 gaaagtgtgt agttattatt gattttattg gaatataatt gtatgaagac ttaaattttt
     7621 ataaactgat gtgtatattg aaaaaaatat tttcctgaat gtagttatag catcttatga
     7681 aaggtgcaaa ttgtaattta aaatttttag tgtataataa gaattgaaaa ttcaaaccga
     7741 taaaaattaa taaaaaataa ttataaatta ataatttggg ggaggaaatg tatgataaaa
     7801 ttaatagcaa cagatttaga tggaactttg ttagatgaaa agagcgaaat aaatccagag
     7861 ttttataaag tatttaaaaa attaagagag agagggataa tgttttctgc tgctagtggc
     7921 aggcagtatc aaaatcttat aaaaaaattt gaagatataa aagatgatat gatgtttata
     7981 tcagaaaatg gaacactagt agtatataaa ggaaaagaaa tattatcaaa tcctttaaat
     8041 aaagaattag taaatgaaat catagagaca acaagaagta taaaagggaa aaaaattgta
     8101 atgagtggga aaaaatacgc ttatatagaa agcaaagatg aagcgtttat acaagaggtc
     8161 agtacttatt atgcaaagtt taaaattgta gaagatttga cgaaagtaga aggagacata
     8221 ttgaagatag ctgtatttga cttcaaaggt gcagaacata ataataatat ctactttgaa
     8281 aagttttctg atagagctca agtatgtatt tctggagtgg agtggcttga tttaactgca
     8341 aaaggtgcaa ataaagggag tgccataaaa aaagtacaaa aaatgttaga tataaaatat
     8401 gaagaaacta tggtatttgg tgaccagtta aatgatgtgg aaatgatgaa aagtgcctat
     8461 catagttatg ccatggaaaa tgcaaatgaa catttgaaac aaattgctag atttagagca
     8521 aaaagaaata ctgaaaatgg cgttgtagat aagataaaag aggttataaa aattggatag
     8581 tcatatgtat agatgaaaaa gattattttg aagaaatcaa aaaaatatca atgtaatttt
     8641 gataaataag tttatttatg cgaaaattac attgatattt aataaacatc aataatagat
     8701 atttattttt ttatgatatt cagtattttg ttttctattt gagttgcatt ttcagcagaa
     8761 attaaataaa catatctacc tgccttctca attatagtat tatttgctat ttcagaattg
     8821 attttttctc catgtccttc tccaaaaggt gcttgtatca tatctttttt ataattttct
     8881 agagttttat agattttatc tacctcgtca gtttttataa cgataatatc ttctagagat
     8941 aatttttcag aagaattaat tacaaaacct tgaattatgt cttcctctac atcattaaaa
     9001 tagttaaaat cctttgcatc aaagattttt gattccttta taagtaaatc agaagatttt
     9061 aatgcttttt ttatttttga tgtagaaacg tttttgtaat caccacttga agaacaacta
     9121 accattccta atgataagat tatcgaaaaa aacaatatta aaattttttt catgataaac
     9181 ctccaagttt tttaaactct gtcttaaatt agtttactac ataattatat aaaaataaat
     9241 gtatttaatg ttacagtttt gattaataat gtaatatttt gataataata cctattgaga
     9301 ggtgattaga aatgagaata ccaaaacatg taggaattat accggatgga aatagaaggt
     9361 gggcaagtca aaatggtatg acaaaagata aaggatatga aagtggaata gaccctggac
     9421 tagagtctta tagattatgt agagatttag gctgtgaaga ggtgactttt tatggattta
     9481 caactgataa tacaagacgt ccatcagtgc agacaaaagc ttttacaaaa gcatgtattg
     9541 atgctgcaaa tatattaata gatgaaggtg ctgctttatt agttgtagga aatacaaaat
     9601 ctccaatgtt tccagaagaa ttattaccat atactaatag aattaaagcc aaagaaggtc
     9661 aaataaaagc aaatcttctg gtaaattatg gttggtattg ggacttaaat aatatatcag
     9721 gagtagaaaa tgtaaataaa aataatatac aggattacat acagtcattt gatgtatcca
     9781 gactagactt gataatcaga tggggtggaa gaaggagatt aagtggtttc cttcctgtac
     9841 aatcaattta tgcagatttt tatgttatag ataattattg gccagatttt aagagtgacg
     9901 atataaagga cgcatttagt tggtatgata cacaagatgt cactttaggt ggataattaa
     9961 aaatattctg atttaaggtt gtagagtgtt ttaaattatt gaaaaaaaaa cttcttgatg
    10021 gtaaaataac actaataata tgtaaaagtg taaaaattta taccaagaag ggtgaagaaa
    10081 tgtcaaaagg caataataac aataattcaa gaaacaaatc taaaaagacc agtcatctga
    10141 atagaaaaaa gcgaaaatta aataagaaaa agttagcagt gttgatttgc tttacagttt
    10201 tatttttatt tattgcattt aaggctactc aaggtgtagt agctttggta aaaagtatgg
    10261 ataaaagtaa taaaactaat caacaacaaa atgttaactc agaacaattt gaccttggta
    10321 atgaagagga aaataaaaag aaaaaatata ctgtatttat agacccaggt catggaggca
    10381 atgataaagg aactgaaagc aagaccagta atagatatga aaaagaccta aatctacaaa
    10441 ttgcaaaaaa attagcaaat aaattatcta aacaaaagga tattcaagta gtagtatcca
    10501 gaactgatga tacatatata tcattaaagg atagagctat cttagcaaat aatagttctg
    10561 ctgatgtgct tgtttctatt catttaaatg cagagaaaaa tggtaataca gcaacaggta
    10621 tagaaacttg gtataggaat aaagctacag atggttcaaa agaacttgca cagacagtac
    10681 aaagtactat agtatcctat gttaaagtta gagatagggg aatagttgaa aataactttg
    10741 aagtacttag agaatcaaat atgcctgcta tattaataga gtgtggtttt ttaacaacac
    10801 cttcagagga acaaaaaata ataaatgaaa aatatcaaga ccaacttgcg gaaggaattg
    10861 ttcaaggtgt actttcatac ctagattcta aagggaataa gtaagttaac tttactttaa
    10921 tgtgaggata aatttaaaag tttaactgtg taaattgtat taactttatt tcgtaatgtt
    10981 gcaaacattg tttgaattta gaaa
//
LOCUS       DAELLV010000121        10951 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.121, whole genome shotgun sequence.
ACCESSION   DAELLV010000121 DAELLV010000000
VERSION     DAELLV010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10951
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.121"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            117..767
                     /locus_tag="KPD58_002362"
     CDS             117..767
                     /locus_tag="KPD58_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HBG0266124.1"
                     /translation="MKAEKTSIYPIIKNNLNKISIVEYKKGQRFVASDKDLQEVFFII
                     EGVALVECTTRGGNKFLVDIVPENEFIGKISYIYEHNLKCDIFAKTNIKLFRFEKNVF
                     EEFYLKPDFIALFHRKCTRRIYELYKTRMVRELFSCTEVIAYCISNKQENNVCNIKAI
                     RNFSEIYSISRKSRYHSLLKLTEKEIVKKMGNTYEILNYDELHKLAFEVKEFLEDE"
     gene            complement(907..2283)
                     /locus_tag="KPD58_002363"
     CDS             complement(907..2283)
                     /locus_tag="KPD58_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG0266125.1"
                     /translation="MQELFSLKDENKRFYKILLSLCIPIIIQNLISTSVNVIDTIMIS
                     SLGETSVASVGVANQFFFLFNMSLSGITGGAGVFISQFYGKKDVSNIRKVTGLTCVLA
                     IVLSFVFVIPALLTPKPIIHIFSYDSEVVKLCIDYFSIAVFSYPLIAVSTVFSTGSRG
                     VRNPKLGMICSAFALVTNVILNYGFIFGNFGLPALGVKGAALATVIARICELILMITY
                     VYLYKKDYILKFGLKNLKAIDKIFIKSFSSKSFPIFVNDSVWAIGTVLYSVAYARAGT
                     SAIAASQIATSTGNFFIMTAVCIASGASIMLGNELGADHIKRAIEYAKKFSILVFSAG
                     LILGIILILNIPLLLKMFSVSDSLASDITKIFFIMGILMALKSFNTLVIIGILRSGGD
                     TKYALFLELGCMWLASIPLTFIAAFKGAPIFVLVLLTYSEEVVKFIFGVPRALSKKWA
                     INIVKEID"
     gene            complement(2299..4212)
                     /locus_tag="KPD58_002364"
     CDS             complement(2299..4212)
                     /locus_tag="KPD58_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG0266126.1"
                     /translation="MIVLSCNNLNKSFGIDSILENISFTVNEGDKIGIIGVNGTGKTT
                     LFKIISGIYGYDSGDIYTSKDCEIGYLEQNTNFYSDNTILEEVLEVFKNLIEMESYLR
                     ELEVKISEESTKTNSPIIEKIMDEYSHKLELFSDLNGYGYKSEAKGVLKGLGFSDNDM
                     DKPISILSGGEKTRVLLGKLLLKKPTLLLLDEPTNHLDSEAIEWLEVFLKQYKGTVML
                     ISHDRYFLDQSVNRIFEVHNKKLKVYNGNYSKFVELSKIEKELELKKFEDQQKEIKKQ
                     EESIERLKAYGREKHLKRARSKEKALDKVDVLDKPEAYRKKARIQFTPSVQSGNDVLQ
                     IRDVSMGYGERILFKDLDLDIYRGEKVALIGANGVGKSTLFKIITNELQPLSGNIKFG
                     TNVHVSYFHQEQKTLNLDNTIIDEIWENNTHLTQTTLRNMLGAFLFVDEEVFKKISTL
                     SGGERARVAILKLILSNANLLLLDEPTNHLDIDSKEVLEEALTNYDGTIFTISHDRYF
                     LNTVVDKILVLDENGITEYLGNYDYYIDKKRQIQEMSIIEEKEEKTRTQIKDEKRKER
                     EQREIEKKNRIKRQNIEKEIEKLEIEIEKLDILLCQEEVYSNPDKAKEVSQEKINLEN
                     NLASLYDEWEEFM"
     gene            4348..4980
                     /locus_tag="KPD58_002365"
     CDS             4348..4980
                     /locus_tag="KPD58_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBG0266127.1"
                     /translation="MLGNKNISMAVIRRLPKYHRYLGDLLDRDIQRISSKELSDIIGF
                     TASQIRQDLNNFGGFGQQGYGYNVEALHTEIGKILGLDRPYNAVLVGAGNLGQAIANY
                     AGFRKAGFEIKALFDANPRMIGLKIREFEVLDSDTLEDFIKNNNIDIAVLCIPKNGAQ
                     EVINRVVKAGIKGVWNFAPLDLEVPKGVIVENVNLTESLFTLSYLMKEGK"
     gene            5119..5658
                     /locus_tag="KPD58_002366"
     CDS             5119..5658
                     /locus_tag="KPD58_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HBG0266128.1"
                     /translation="MVFVEELWKKAIEFHGHECPGLAIGFKAALAAKKYLGCKQSGDE
                     EIVCISENDACGIDGIQVVLSCTMGKGNLLLRMTGKSVYHFFNRENGKSIRIYVKSKP
                     FEMDREGYKRYLLESDFEEIFEITDTKLRLPEKARIFKSLKCEVCGEFASEQYIRIEE
                     GKKVCLDCFDKYERFYEEY"
     gene            complement(5834..6004)
                     /locus_tag="KPD58_002367"
     CDS             complement(5834..6004)
                     /locus_tag="KPD58_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG0266129.1"
                     /translation="MAYKITDACISCGACEAECPVSCISAGDDAYVIDAGSCIDCGSC
                     AGACPVDAPQPE"
     gene            6289..7356
                     /locus_tag="KPD58_002368"
     CDS             6289..7356
                     /locus_tag="KPD58_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HBG0266130.1"
                     /translation="MKNKLVYICIMSLLLFVVGCSNNPNKESTIKKDSKNNQSQVKEE
                     KREPVKKDITISFAGDVTMGNYKGSSYYGSFDHEFKRQGEDYNYFLKNVKPVFEKDDL
                     TVVNLEGPLTTASNAKEKKFAFKGKPGYVNILKSGSVEAVSIANNHSEDYFEEGMKDT
                     QFILDENKINYFGLGKDAVVDVKGIKVGLLGYNGLSTEYNEKNLKQMEDDIKSLKKKS
                     DVVVVYFHWGIELDYYPDKKQKDFAHYAIDKGADLVMGSHPHVIQGIEKYKNKYIAYS
                     LGNFCFGGNKNPSDTDSYIYQQTFTFSDNKLTSIKEPNIIPTSITSSNSRNNYQPKIL
                     DGSEKDRVLNKLEKISKDLNK"
     gene            7526..9427
                     /gene="cooS"
                     /locus_tag="KPD58_002369"
     CDS             7526..9427
                     /gene="cooS"
                     /locus_tag="KPD58_002369"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HBG0266131.1"
                     /translation="MACNTCKMCESADKKLESFVASKDVETAFHRTEDQKVKCGFGLQ
                     GVCCRLCSNGPCRVTPKSPRGICGADADTIVARNFLRAVASGAACYLHVVENTAKNLK
                     NVGITKGVVKGEKTLNQLAEMFGIESDEKYDKCIKVADKVINDLYKSRDDKMELVEKI
                     AYAPRVKKWKELGIMPGGAKSEVFDAIVKSSTNLNSDPVDMLVNCLNLGISTGLYGLT
                     LTNLLNDVMLGEPVIRMAPVGFNVIDPDYINIMITGHQHSTFANFQDRLKDEDVIKLA
                     QSVGAKGFKLVGCTCVGQDLQLRGEHYQEVFAGHAGNNFTSEAVLSTGAIDIVLSEFN
                     CTIPGLEPIADKYKVKMVCLDDVAKKANAECIGLDRSKLDELSNTLIEKALESYKERR
                     GSIEIDIPKDHGFEQSLTGVSEKNLKAFLGDSWKPLINLIAEGKIKGVAAVVGCSNMT
                     AGGHDVNTVELTKELIKKDIIVLSAGCSTGGLENVGLMSPGAEELAGENLKEVCKTLG
                     IPPVLNFGPCLAIGRLEIVATELAAELGIDLPQLPLVLSAPQWLEEQALADGAFGLAL
                     GLPLHLALPPFITGGKLVTEVLTEKLKDLTGGHVIVNPDPKSSANQLEEIIIDRRKNL
                     GLKDVECNA"
     gene            9420..9893
                     /locus_tag="KPD58_002370"
     CDS             9420..9893
                     /locus_tag="KPD58_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG0266132.1"
                     /translation="MHRIFINKDLCTGCKSCVLACMLKHNKDYDMYTLDLENIDNDSR
                     GHIELDSRHNNPVPILCRHCDEPECVLACMSGAMHKDSESGIVSYDEEKCGSCYMCVM
                     SCPYGLLKPDDRSKQNILKCDLCKDEEYPRCVANCPSGAIELQKEENDELCSVRG"
     gene            9868..>10951
                     /locus_tag="KPD58_002371"
     CDS             9868..>10951
                     /locus_tag="KPD58_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG0266133.1"
                     /translation="MNYVVLGASAAGINAVKTLRELDKDSNIVVISKDENVYSRCMLH
                     HVISEHRTLKQINFVDEDFMEQNNVKWIAGKTVKGIDINKKVVQTEDITVNYDKLLIA
                     TGASSAIPPIKNLRDGNFVYSVRNIDDIYKIKEKAQNSKNVVIIGAGLVGIDALVGLF
                     KYEQLNISVAFMEKYILDRQLDEYTASVYENKFKEKGVKFYPSASIQEIVLDNSKNVT
                     GVAFSNGEVLDADMVIVATGVKPNADFLDGTGIEYDRGIIIDDMCQTTQKDIYAAGDV
                     VGKNAIWPLAVKQGIVAAYNMVGKDKKIEDEFAFKNSMNFMDIPTISIGMNTPVDDSY
                     KVLTRHGINDYKKFVFKDNVIYGAVIQ"
BASE COUNT         4189 a         1353 c         1797 g         3612 t
ORIGIN      
        1 agaatgaatt tgtcaagttt aatttagttg taattttatc ttttattaaa aataatataa
       61 attaaaacat ataaataaaa tattttatac aatatattgt aaaggaggta actttgatga
      121 aagcagaaaa aacaagtata tatccaatta ttaaaaataa tttaaacaaa atatcaattg
      181 ttgaatataa aaagggtcaa agatttgtag catcagataa agacttacaa gaagtgttct
      241 ttataataga gggagttgca ttagtggagt gtactactag aggaggaaat aaatttcttg
      301 ttgacattgt acctgaaaat gaatttatag gcaaaattag ttacatatat gaacataatt
      361 tgaaatgtga tatatttgca aagactaata taaaattatt tcgatttgaa aaaaatgtat
      421 ttgaagagtt ttatctcaaa ccagatttta ttgcactatt tcatagaaaa tgtacaagaa
      481 gaatatatga attatataaa accagaatgg taagagaact attttcttgc acagaagtta
      541 ttgcctattg tatatcaaat aaacaagaaa ataatgtctg caatataaaa gctattcgta
      601 atttcagtga aatctattca attagtagaa aaagtagata ccattctctg ctaaagttaa
      661 ctgaaaagga gatagtaaaa aaaatgggta atacatatga aattttaaac tatgatgaat
      721 tacataaatt ggcatttgaa gtaaaagaat ttttggaaga tgagtaaaaa ttgttactta
      781 atcaaaataa agtataataa tttctgtaaa aaatagaaat agtctcactg aaaatcaaaa
      841 atactttagt tattttcaat gagactgttt agaaatattg tatacttata atgtatagta
      901 taattattaa tcaatttcct ttactatatt aatagcccat tttttagata atgctctagg
      961 aacaccaaat ataaacttaa ccacttcttc actataagtg agcaaaacga gtacaaatat
     1021 aggtgcacct ttaaatgcag ctataaaagt tagaggaata gaagctaacc acatacatcc
     1081 taattctaaa aatagagcat atttagtatc tcctccactt cttaaaatac caattataac
     1141 caatgtatta aaagatttaa gagccattag tattcccatt ataaagaata tcttagttat
     1201 atcagaagct aagctatcag aaacactaaa catttttaat aataatggta tatttaaaat
     1261 taaaataatt ccaagtatta aaccagctga aaatacaagt atagaaaact ttttagcata
     1321 ttcaatggct cttttaatat ggtcagctcc aagttcattt ccaagcataa tagatgcacc
     1381 agatgcaata catacagctg tcattatgaa gaaattacct gtactggttg ctatctggct
     1441 agctgctata gcagaagtac cagctcttgc atatgcaaca gaatacagga cagttccaat
     1501 tgcccataca gagtcattta caaatatagg aaagcttttt gatgaaaaag attttataaa
     1561 aattttatca atagctttta gattttttag accaaacttt aatatatagt cttttttata
     1621 aaggtaaaca taagtaatca ttaaaattaa ttcacatatt ctagcaataa cagtagctag
     1681 agcagcacct tttacaccca atgcaggaag accgaagtta ccaaatataa atccataatt
     1741 taaaataaca tttgttacaa gtgcaaatgc actacaaatc atacctaact taggatttct
     1801 aacacctctt gaaccagtac taaatacagt acttacagca attaatggat aactaaatac
     1861 tgcaatacta aagtaatcta tacaaagttt taccacctca gaatcatacg aaaatatgtg
     1921 tataattggc tttggtgtta gtaaagcagg aataacaaat acaaaactta aaacaattgc
     1981 caatacacaa gtaagacctg tgacttttct tatattactc acatccttct ttccataaaa
     2041 ctgtgaaata aaaactccag cacctcctgt gataccagat aaggacatat taaaaagaaa
     2101 gaaaaattgg tttgcaactc caactgaagc tacagatgtt tcccccaaac tacttatcat
     2161 aattgtatct attacattta cagaagtaga aattaagttc tgtatgataa taggtataca
     2221 taaagataga agtattttat aaaatctttt attttcgtct tttaaagaaa atagttcttg
     2281 caaataatcc tctccttttt acataaattc ttcccactca tcatatagag aggcaaggtt
     2341 attttctaaa ttaatttttt cttggctaac ttctttagct ttatctggat tagagtatac
     2401 ttcttcttga cataaaagta tatctaattt ttcaatttca atctctaact tttcaatttc
     2461 tttttctata ttttgtcttt ttattctatt tttcttttct atttctcttt gttctctctc
     2521 ttttcttttt tcatctttta tttgagttct agttttttct tctttttctt ctataatact
     2581 catctcttgg atttgccttt ttttatctat ataataatca taatttccca gatattcagt
     2641 aattccattt tcatctaaaa ctaaaatttt atcaacaaca gtatttagaa agtatctatc
     2701 atgtgatatt gtaaatatag tcccatcata attagttaag gcctcttcaa ggacttcctt
     2761 agaatcaata tctaggtgat tagtaggctc atctaataac aataagtttg catttgatag
     2821 tataagttta agtatggcaa ctctagctct ttctccacca ctcaatgtag aaatcttctt
     2881 aaaaacttct tcatctacaa ataaaaatgc ccctaacata tttctcaaag tagtttgagt
     2941 aagatgagta ttattttccc aaatctcatc aattattgtg ttatctaaat taagagtttt
     3001 ttgttcctga tggaagtagg aaacatgtac attagttcca aatttaatat tgccactaag
     3061 aggttgtagt tcattagtta ttattttaaa taatgtagat ttgccaacac catttgcacc
     3121 tataagtgca actttttccc ctctatatat atctaaatcc aagtctttaa aaagtattct
     3181 ttctccataa cccatagata catctctaat ttgaagaaca tcattaccac tctggacaga
     3241 aggcgtaaat tgtatacgag cttttttcct ataagcttct ggtttatcta aaacatctac
     3301 tttgtctaat gctttttctt tacttcttgc acgctttagg tgtttttctc taccataagc
     3361 ttttagtctt tcaattgatt cttcttgctt ttttatttct ttttgttggt cttcaaattt
     3421 cttaagctct agttcttttt ctattttaga taactcaaca aatttagagt agttaccatt
     3481 atatacttta agctttttat tgtgaacttc aaaaattcta tttacggatt ggtctaagaa
     3541 atatctatca tgagatatta acatgacagt ccctttatat tgttttaaaa atacttctaa
     3601 ccattctatt gcttcagagt ctaagtggtt agtaggttca tcaagtaaca atagagttgg
     3661 tttcttaaga agtaattttc ctaaaagaac tctagttttt tcaccaccag aaagtatgct
     3721 tattggctta tccatatcat tgtcactaaa tcctaatccc tttagaactc ctttggcttc
     3781 agatttatat ccatatccat ttaaatctga aaataattct agtttatgtg aatattcatc
     3841 catgattttt tctataattg gagagtttgt ttttgtactt tcttcagata ttttaacctc
     3901 caattctctt aaataggatt ccatttctat caaattttta aaaacttcta gtacttcttc
     3961 caaaatagta ttatccgaat aaaaattggt attttgttct aaatatccga tttcacaatc
     4021 tttagaagtg taaatatctc cactatcata tccataaatt cctgaaatta ttttaaataa
     4081 agtagttttt ccagtaccat taacaccgat tataccaatt ttatcgcctt catttactgt
     4141 aaaactaata ttttctaata tagaatctat accaaaactt ttatttaagt tattacatga
     4201 caaaacaatc ataaatttct acctccttga actatctaaa attataatgt tttgatataa
     4261 tataatcaag gttatatgta ttagttttga aaaagaaata ttttattgtg aatcaaacaa
     4321 aaaagaggac acttttagga ggtgaagatg ttgggaaata aaaatatatc aatggcagtt
     4381 ataagaaggc tcccaaaata tcatagatat cttggagact tattagatag ggatatacaa
     4441 agaatatctt ctaaagaatt gagtgatata atagggttta ccgcttctca aataagacaa
     4501 gatttaaaca actttggtgg atttggacaa caaggatatg gttataatgt agaagctctt
     4561 catactgaga taggtaaaat tcttgggttg gatcgaccat acaacgcagt tcttgtagga
     4621 gcaggtaact taggacaagc tatagccaat tatgcaggat ttagaaaagc tggattcgag
     4681 ataaaagctt tatttgatgc aaatcctaga atgataggtt taaagataag agagtttgaa
     4741 gtattagatt cagatacttt agaagacttt ataaaaaaca ataatataga tattgctgta
     4801 ttatgtatac ctaaaaatgg agcacaagaa gttattaata gagttgtaaa agctggaatc
     4861 aaaggtgtat ggaattttgc acctttagat ttagaagttc cgaaaggtgt tatagttgaa
     4921 aatgtaaact taacagaaag tttatttacc ttatcgtatt taatgaaaga aggaaagtag
     4981 acaacttaaa catattaata atgagcagtt gtaaagagta ttgagaaagt cagaagattt
     5041 aatattaaat cctctggctt tttgaaatat taggataaaa atttaaataa tgtatggaaa
     5101 taaacaatat taaggagagt ggtttttgtg gaggaattat ggaaaaaggc aattgaattt
     5161 catggtcatg aatgtccagg gttagctata ggatttaaag cagcacttgc agcaaaaaaa
     5221 tatttaggat gtaaacagtc aggagatgag gaaattgtat gtatttctga aaatgatgct
     5281 tgtggaatag atggtataca agtagtgcta agttgcacaa tgggtaaggg aaatttacta
     5341 cttaggatga ctggaaaatc agtatatcat ttctttaata gagaaaatgg aaaatctata
     5401 aggatatatg taaagtcaaa accatttgag atggatagag aaggatacaa aagatattta
     5461 ttagaaagtg attttgaaga aatatttgag attacagata ctaagttaag acttcctgaa
     5521 aaagcaagaa tatttaaatc ccttaaatgt gaagtgtgtg gtgaatttgc atctgagcaa
     5581 tatataagga ttgaagaagg aaagaaagta tgtcttgatt gttttgataa atatgaaaga
     5641 ttttatgaag agtattaaaa ttataaatta gattaaagtt aaaagtatta gatatgtgct
     5701 aacagtattt taagatgaat taggtagcaa cataataaag tatataaata atattaattg
     5761 aatcatacat aattaaaaag aggctgatta gtatttataa aataaaatca gtctctttat
     5821 gaataacgaa ttattattct ggttgaggtg catctacagg acaagctcct gcacaagaac
     5881 cacagtctat acaagaacca gcatctataa catatgcgtc gtctccagct gatatacagc
     5941 taacagggca ttcagcctca caagcaccac agcttataca agcgtcagta attttatatg
     6001 ccattttgaa atcctccctt ttatatgtat tgtaattcaa taacattata acaaatatta
     6061 ttgaatataa caattaaatt aattctacaa caaaaacttt attatgatat tcttgtttta
     6121 aagttttaca ataaagttta atctgcttat atttttattt attttaaaga agtttatttt
     6181 aaaaataaaa ttaaatttaa aaagttatgt attgattata tttaaaaatg ttatactcta
     6241 aaaaagtata ttttatttaa aaatttatta atacaaagga gatttactat gaaaaataaa
     6301 ttagtttaca tatgtattat gagtctattg ctatttgtag taggatgttc taataatcca
     6361 aataaggaaa gtaccattaa aaaagactct aagaataacc aaagccaagt taaagaagaa
     6421 aaaagagaac ctgtgaaaaa agatataact ataagtttcg ctggtgatgt aactatgggg
     6481 aactataaag gttcatcata ttatggcagt tttgaccatg aatttaaaag acaaggagaa
     6541 gattacaatt attttcttaa aaatgtaaaa cctgtatttg aaaaagatga cttaacagtg
     6601 gttaatttag aaggtccact tactacagcg tcaaatgcaa aagaaaaaaa atttgcattt
     6661 aaaggaaagc ctggatatgt aaatatttta aaatctggaa gtgtagaggc agtaagtatt
     6721 gcaaataatc attcagaaga ttattttgaa gaaggaatga aagatacaca gtttatattg
     6781 gacgagaata aaataaatta ttttggatta gggaaagatg ctgttgtaga tgtcaaagga
     6841 attaaagtgg gtctattagg gtataatgga ttatcaactg aatataatga aaaaaattta
     6901 aaacaaatgg aagatgatat taagtcctta aaaaagaaat cggatgttgt tgtagtgtac
     6961 tttcattggg ggatagaatt agattattat ccagataaaa aacaaaaaga ttttgctcat
     7021 tatgctatag acaagggagc agatttagtt atggggtctc acccacatgt gattcaagga
     7081 atagaaaagt ataaaaataa gtatatagct tatagtctag ggaatttttg ctttggagga
     7141 aataagaatc cttctgatac agatagttat atatatcaac aaacctttac tttttcagat
     7201 aataaattaa cttcaattaa agagccaaat ataataccaa cttcaataac atctagtaac
     7261 tccagaaata attatcaacc aaaaatattg gatggaagtg agaaggatag agttttaaat
     7321 aaacttgaaa aaataagcaa agatttaaat aaataaaatg taaaaaaaaa taaatatata
     7381 tatttatttt aataatttat attttgttta gggtatggta aatcgtggta ttaaataagt
     7441 agattttaat agttaaatta tagtcaatta taataaagct atttaatcta cttttttaat
     7501 ataaaaatat aagggggaat ttgtaatggc ttgtaacact tgtaaaatgt gcgaaagtgc
     7561 agacaagaaa ttagaaagtt ttgtagcttc aaaggatgtt gaaacagcat ttcatagaac
     7621 agaagatcag aaagttaagt gtggttttgg acttcagggg gtttgttgta gactctgttc
     7681 aaatggacca tgtagagtaa ctcctaaatc accaagaggt atatgtggag cagatgcaga
     7741 cactatagta gcaagaaact ttttaagagc agtagcatca ggagcagctt gttatttaca
     7801 tgtggtagaa aatacagcta agaatttaaa aaatgttgga ataactaaag gtgttgttaa
     7861 aggagaaaaa acattaaatc aattagcaga gatgtttgga atagaaagtg atgaaaagta
     7921 tgataaatgt ataaaggttg cagacaaagt tatcaacgat ttgtataaat caagagatga
     7981 taaaatggaa ttagtagaaa aaatagctta tgcacctaga gtgaaaaagt ggaaagagtt
     8041 aggtataatg cctggtggtg caaagtcaga agtttttgat gcaatagtaa aatcatcaac
     8101 aaatttaaac agtgacccag tagacatgct agttaattgt ttgaacttag gtatatcaac
     8161 aggattatat ggtcttacac taactaatct tttaaatgat gttatgttgg gagaaccagt
     8221 tataagaatg gctcctgtag gatttaatgt aatagaccca gactacataa atataatgat
     8281 aacaggtcat caacattcaa catttgcaaa tttccaagat agattaaaag atgaagatgt
     8341 tataaaatta gctcaatctg ttggcgcaaa aggatttaag ttagtaggat gtacatgtgt
     8401 tggtcaagat ttacaattaa gaggagaaca ttatcaagaa gtatttgctg gacatgcagg
     8461 taacaatttt acaagtgagg ctgttttatc aactggagca atagatatag ttctttcaga
     8521 atttaactgt actataccag ggcttgaacc aatagcagat aaatataaag taaaaatggt
     8581 ttgtttagat gatgttgcta aaaaagcgaa tgcagaatgc ataggtttag acagaagtaa
     8641 attagatgaa cttagtaaca ctttaataga aaaagcattg gagtcttata aggagagaag
     8701 aggaagtatt gaaatagata taccaaaaga ccatggattt gaacaatctt tgactggtgt
     8761 aagtgaaaag aacttgaaag cgtttttagg agattcatgg aaacctctta ttaatttaat
     8821 tgcagaagga aaaattaaag gtgttgcagc tgttgtagga tgttcaaata tgacagcagg
     8881 aggacatgat gtaaatactg ttgaacttac taaagaattg attaaaaaag atataatagt
     8941 actatctgca ggatgttcta ctggaggatt agaaaatgtt ggtcttatgt cacctggagc
     9001 agaagaattg gcaggagaaa acttaaaaga agtatgtaag actttaggaa taccaccagt
     9061 attaaacttt ggaccatgtc tagctatagg aagactagaa atagtagcaa cagaattagc
     9121 agctgaactt ggaattgacc taccacaatt accattagtg ctttcagctc cacaatggtt
     9181 ggaagaacaa gcattagctg atggagcttt tggtcttgcc cttggcttac ctcttcattt
     9241 agctttacct ccatttataa caggaggaaa attggtaaca gaagtattaa cagaaaaatt
     9301 aaaagactta acaggaggac atgttatagt gaatccagac cctaaatctt cagctaatca
     9361 attagaagaa ataataatag atagaagaaa aaatttaggg ttaaaggatg tggaatgcaa
     9421 tgcatagaat ttttataaat aaagatttat gtacaggatg taagagctgt gtactagcat
     9481 gtatgctaaa acacaataaa gattatgaca tgtacactct tgatttagaa aatatagata
     9541 atgatagtag agggcatata gaattagata gtagacataa taatccagtt ccaattcttt
     9601 gtagacactg tgatgaacct gaatgtgtat tagcatgtat gagtggtgca atgcataaag
     9661 atagtgaaag tggaatcgtt tcatatgatg aagagaaatg tggttcatgt tatatgtgtg
     9721 taatgtcttg tccatatgga ttattaaagc cagatgatag aagcaagcag aacatactta
     9781 aatgtgattt atgtaaagat gaggagtatc ctagatgtgt agctaattgc ccaagtggag
     9841 caattgaatt acagaaggag gaaaatgatg aattatgtag tgttaggggc tagtgcagct
     9901 ggtataaatg cagtaaaaac attaagagaa ttagataaag attcaaatat agtagtaatt
     9961 tctaaagatg aaaacgtata ttcaagatgt atgttgcatc acgttatatc tgaacacaga
    10021 actttaaaac aaataaattt tgttgatgaa gattttatgg agcaaaataa tgtaaaatgg
    10081 attgcaggta agactgttaa aggtatagac ataaataaaa aggtagttca gacagaagac
    10141 attactgtaa attatgataa acttttaata gctacaggtg catcttctgc tattcctcct
    10201 ataaaaaatt taagagatgg aaattttgta tattctgtga gaaacataga tgatatttat
    10261 aaaataaaag aaaaagcaca aaattctaaa aatgtagtta taataggtgc tggtttagtt
    10321 ggtatagatg cattagtggg attatttaaa tatgagcagt taaatatttc tgtagctttt
    10381 atggaaaagt acattttaga tagacaattg gatgaatata cagcatcagt gtatgaaaat
    10441 aagttcaaag aaaaaggtgt aaaattttac ccaagtgcat caatacaaga aattgtattg
    10501 gataacagta aaaatgttac aggtgttgca ttttcaaatg gtgaagtttt agatgcagac
    10561 atggtaattg tagctacagg ggttaaacca aatgctgact ttttagatgg aacaggaata
    10621 gaatatgata gaggtataat aatagatgat atgtgccaaa caacacaaaa agacatatat
    10681 gcagcaggag atgttgtagg taaaaatgct atttggcctc tggcagtaaa gcaaggaatt
    10741 gtagctgcct ataatatggt tggaaaagat aagaagatag aagatgaatt tgcttttaaa
    10801 aatagtatga atttcatgga tataccaact atttctatag gaatgaatac acctgtagat
    10861 gatagctata aagtattaac cagacatgga attaatgatt ataaaaagtt tgtttttaaa
    10921 gataatgtaa tctatggagc agttatacaa g
//
LOCUS       DAELLV010000122        10827 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.122, whole genome shotgun sequence.
ACCESSION   DAELLV010000122 DAELLV010000000
VERSION     DAELLV010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10827
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.122"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            152..1000
                     /locus_tag="KPD58_002372"
     CDS             152..1000
                     /locus_tag="KPD58_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBG0266134.1"
                     /translation="MNNQKNLNIPITISSYTLGTEVSFRDRVRIAKEAGFDGIGLRAE
                     NYIDAKNAGVTDEEMIAILNEYDMKVTEVEYITQWGREEDRTEAQREKEQNVYHMAHL
                     FNVKHINCGLLEMIPEEQIITALGELCDRAGELIIGLEFMPYSGVPDLATAWRVVKGC
                     NRENAMLILDTWHWARAKQTAEDLAPVPADKIVSIQVCDVLETPYEKLRDESLHDRLA
                     PGEGYGNTEEFVKIIKEHNISPRVIGAEVISDSLISKGLSTAAETVFNATKKVLDKAW
                     SEVSPK"
     gene            1079..3001
                     /locus_tag="KPD58_002373"
     CDS             1079..3001
                     /locus_tag="KPD58_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG0266135.1"
                     /translation="MKISSMFTPIRIGSMTVPNRFVVPPMGNNFANTDGTLSETSKAY
                     YLERALGGFGLITIESSVVDKKAKGGPRKPCLYDDSTIDSFKNVIDACHDAGSKVSIQ
                     LQHAGSEGNEKVAGHPLKAASAIPASNGRNTPLAITTEEIYELIESYGDAALRAQKAG
                     ADAVEVHCAHGYLVSSFISQRTNKRVDEFGGCFENRMRLPRLIIENIRKKVGNSLAIL
                     CRINSTDDVPGGIDVHDSSVIAAYLEDCGIDGLHVSRSIHIHDEYMWAPTTLHAGFSA
                     ELVTEIKKAVSIPVITVGRYTEPQFAELMVRQGRCDLVAFGRQSLADPEMPNKAKNGK
                     LDEMIPCIACLQGCVPNMFQGKPIACLANPILGHEAELKPAEISKEVLVVGGGVGGML
                     AAWVCAKRGHNVTLVEKSEVLGGQMRLAAYPPGKGDITNLVRSYISKCNQYGVKICTN
                     TEATVELIKEKSPDVVIIATGATPLVLPIPGINDSGLIHAVDLLDGKKSCGKKVLVVG
                     GGMVGCEVAAFLGEQEHEVTVIELREEVGADVISEHRKFLMNDFENYNIHTITGAKVS
                     KFYDDGVDYLLTDGTEHDLKGFDSVVLAMGSRNYDPLSEVIKEVVKETYIVGDAVKAR
                     RALDATKEAFEVALNI"
     gene            3083..3946
                     /gene="aroE"
                     /locus_tag="KPD58_002374"
     CDS             3083..3946
                     /gene="aroE"
                     /locus_tag="KPD58_002374"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBG0266136.1"
                     /translation="MEISGRTGLFALIGTPVGHSKSPVMYNYSFKKLDLDYRYLAFDI
                     TVDKVKEALLAIKTFNIKGANVTMPCKSAVTEYMDELSPAARIIGACNTIVNDNGKLV
                     GHITDGVGYVRNLKENGVEVKGKKITIMGAGGAATAIQVQCALDGAREISIFNPKDDF
                     YKRAEQTVENIKKDVPECVVNLYDLEDTNKLYEEIQSSDILTNATLIGMKPYDNETNI
                     KDTSVLRKDLVVTDVVYNPKKTKMIEDAEANGCKAIGGLGMLLYQGAEAFNLYTGLEM
                     PVEEVNELCFK"
     gene            4146..5363
                     /locus_tag="KPD58_002375"
     CDS             4146..5363
                     /locus_tag="KPD58_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0266137.1"
                     /translation="MNNKKYYPTAIALYFSYFLLGIGISILGQYKPEFSSMWGAKTLS
                     DGTLDISIVLAVIAALGLGRLISYPFAGPISDKYGRKVSGLIGNFLHAIFFVGIVFSP
                     NFYIAYVFAIIGGAANSFLDTCVTPSCMEIFASLGTIANMFTKFTIALAQFLLPFIIG
                     FVAANSISFKVIFIVTAILIVVDAFLIAVLPFPPANNVIDNKGKTVKSEKMKFTPTSI
                     ALVCIGFTCTSTFVLWLNCNQELGKLYGMADPTKIQSFYSMGVICAVLITSLLIKKYI
                     KPIRILVIYPIIALLMLLVVYFVQTPTICMLGGFVIGYSAAGGVLQLTTSTANEMFPT
                     NKGKITSIVMIASSIANYVILNIAGIITKSGGVNGPKYVVLFNVAITFVGILLALFVN
                     MRYEKEKVYDYDV"
     gene            5599..7464
                     /locus_tag="KPD58_002376"
     CDS             5599..7464
                     /locus_tag="KPD58_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HBG0266138.1"
                     /translation="MENVFKRLQEFNGYDGYKESFEMNYLCIYESIPLREQVELANNL
                     VDEILNMYKSESNEIYLLEGSNSKSLICYFEIFMKKINTLVKEMIIDEKWLYKLTKEL
                     IYKSKKVEYVKLGLVLSEKYLNVENLREVVDTFSKSGEYVFYLSNTIKKLEFYNTYLF
                     NLSKKATGSIKVFAIVNMENLDSKINSYLIEDGYKDTKYERLLMNYIISIVDLNEYLE
                     KRDLDKEKINNLARLICNYLLSVEFKYIGNKLELVNRFLPTVVNYGTNFESLYSIFLI
                     AINVLKDENIECNKIEFEKEINGILLSEKWKNIYFEALRDASGKTEDIIKMSEIYDVN
                     LSFDDLLPYLNRDIRDFEVYWHISKKGTTSSRLKLLNFFEETFKIDDLIGKMKDIEKD
                     KLTQEYYDDMLFFIVLKGSKSLYPEGKNISLKGIFGNINEVRKESINILKRYREKLSL
                     EELKIVKEAYEKEKNVILKDELRRVLYESNNLKKEFVNIEKIKVDEHGKDIYLTSIAV
                     AGSRFRNREYLEKELEKSKIYYLTREKDNLYDEKAIKIVGETGYVIGYVPRKENYILS
                     NLLDGGKLLYCRVTEYNLYEDCIYANVYLSYKDVIETVENSLKMVLDKSRIKLIN"
     gene            complement(7642..9006)
                     /locus_tag="KPD58_002377"
     CDS             complement(7642..9006)
                     /locus_tag="KPD58_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0266139.1"
                     /translation="MSQLLIKNAYLITMNADREVYENGSILIENNIIKEVGKFDINIV
                     DKDAEIYDAKGKILMPGLVNTHVHLSQQLGRGLADDVVLLTWLRERIWPYESSFNYED
                     SLISSTACCVELIKSGVTTFLESGGQYVDAMVEAVDKTGLRACLAKSVMDTGDGLPEA
                     WQKTTDEEINTQLDLFHKFNNTSNERIKIWFALRTIFNTTEELLVRTKELADKYNTGI
                     HMHIAEIADEISFVKQNNGVGTVEYLDKLGVLGPNLLAAHTVWLTHKEIDLFRLHDVK
                     VSHDPGSAMKVVLGFASIPEMLEKGIPVSIGTDGAPSNNRMDLMRDMYLTSLIHKGRT
                     LNPTVVPAEEVLEMATINGAKCALLEKEIGSLEVGKKADMIILNPDTIHCLPMHNPIG
                     NIVYSMTSENVDSTICDGKWLMKERKVLVVDESELLNKVKKQASKIKDKAEVKIPSKF
                     KIIK"
     gene            complement(9185..10528)
                     /locus_tag="KPD58_002378"
     CDS             complement(9185..10528)
                     /locus_tag="KPD58_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBG0266140.1"
                     /translation="MVPNTNNVQPGGNNSSLSFLDKMFKFSERGSSLKTEVIGGVTTF
                     LTMAYIVFVNPAILSEAGMDKAALITVTILATAIGTLIFAILGNAPFALAPGMGLNAF
                     FTYSLVIGAGIPWQTGLGVVFLSGCVFFTLAVTGLRKTIADAIPRELAVASSAGIGLF
                     LAFVGLKNMGVIIANPDTIVSLADFTPTVLIALFALILMAILEYKKVKGGILISIVVA
                     TIISVIFGFVELPTAIISTPPSIAPVAFKLDIVGALKFSLIGPIFSFMFIDMFDSLAF
                     LITCCKQMGLEDENGEIKDLGRMLYADVSSTLIGSFLGTSTVTTFGESASGIAAGART
                     GLASIVTAALFLATLIFTPLLGIVPTYAAAPALVMVGVFMFESIKQIDFADSKIAVPA
                     FVTVLLMPLTYSISIGLCFGFISYIIMHVVAKETDKISITLWIIGLLAVVNLVLS"
     regulatory      complement(10601..10702)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
BASE COUNT         4017 a         1364 c         1846 g         3600 t
ORIGIN      
        1 gtgatacaat aaatttataa aatctagata aatagaaatc aaaaaatact tttattttta
       61 aggtaaaata atactactag taggataaat aaaaatataa aatcaaaaat actattaatt
      121 ataaagaaaa taaagggggc aaatttaagc tatgaataat caaaaaaatc ttaatattcc
      181 aattacaatt agttcttata cattgggaac agaagtttca ttccgtgata gagttcgtat
      241 tgcaaaagaa gctgggtttg atggtattgg attgcgtgct gaaaattata ttgatgcaaa
      301 aaatgcaggt gttacagatg aagaaatgat tgcaattctt aatgaatatg atatgaaagt
      361 tacagaagta gaatatataa ctcaatgggg aagagaagaa gatagaacgg aagctcaaag
      421 agaaaaagag caaaatgttt atcatatggc tcacttattt aatgttaaac atataaattg
      481 tggattatta gagatgatac cagaagaaca aattataact gcattgggag aattatgtga
      541 cagagcaggt gaacttatta taggtcttga gtttatgcca tatagtggtg taccagattt
      601 ggctactgca tggcgtgttg tgaaaggatg taatcgtgaa aatgcaatgc ttatattaga
      661 tacttggcac tgggctagag caaagcagac agcagaggac cttgcacctg ttccagcaga
      721 taagatagta tcaattcaag tgtgtgatgt tttagaaact ccttatgaaa aacttaggga
      781 tgaatcacta catgaccgtt tggctccagg tgaaggatat ggaaacacag aggaatttgt
      841 gaaaataata aaagaacaca atatatcacc aagagtcata ggggcagaag taatatctga
      901 ttctctaata tctaaagggt tatccactgc tgctgaaact gtatttaatg caactaaaaa
      961 agttttggat aaagcatggt ctgaagtatc tccaaaataa acttatataa tataaaaata
     1021 tacagagtat tataatataa aacttttaaa tataaaactt gaaaggaagg aaaaccttat
     1081 gaagattagt tctatgttta cacctataag aattggttca atgacagttc caaatcgttt
     1141 tgtagttcct cctatgggaa acaattttgc aaacacagat ggtactttaa gtgaaacttc
     1201 taaggcttat tatcttgagc gtgctttagg tggttttgga ttaataacta tagaatctag
     1261 tgttgtagac aaaaaagcaa aaggaggtcc tcgtaagcct tgtttatatg atgatagcac
     1321 aatagatagt tttaaaaatg taatagatgc ttgtcatgat gcaggttcga aggtatctat
     1381 acaattacaa catgctggtt ctgaaggaaa tgaaaaagta gcaggacatc cattaaaagc
     1441 tgcttctgca atacctgcat caaatggtcg taatacacca ctagcaataa caacagaaga
     1501 aatatatgaa cttatagaat cttatggaga tgctgcatta cgtgcacaaa aagctggtgc
     1561 agatgctgtt gaagttcact gcgctcatgg atatctggtt agtagtttta tatcacaaag
     1621 aacaaataag cgtgtagatg agtttggtgg atgttttgaa aatagaatga gattacctcg
     1681 tcttatcatt gaaaatatac gtaaaaaagt aggaaattct ctagcaatat tatgtcgtat
     1741 aaacagcact gatgatgttc caggaggaat tgatgtacac gatagttcag taatagcagc
     1801 atatctagaa gattgtggaa tagatggact tcatgtttca cgtagtatac atatacatga
     1861 tgaatatatg tgggctccta cgactttaca tgctggattc agtgcagaat tagttactga
     1921 aataaaaaaa gctgttagta taccagtaat aacagttgga cgttatacag agccacagtt
     1981 tgctgagtta atggtcagac aaggaagatg tgatttagta gcattcggta gacaaagctt
     2041 ggctgaccca gaaatgccta ataaagctaa aaatggaaaa ctagacgaaa tgataccatg
     2101 tattgcatgc ttacaaggat gtgtaccaaa tatgttccaa ggcaaaccta tagcctgtct
     2161 tgcaaatcct atattaggac atgaagcaga gcttaagcca gctgaaatta gcaaagaagt
     2221 tcttgttgtt ggtggaggtg ttggaggtat gcttgcagca tgggtatgtg ctaaaagagg
     2281 acataatgta actttagttg aaaaatctga agttcttggt gggcaaatgc gtttagctgc
     2341 atatcctcca gggaaaggag atattactaa cttagttaga agttatattt caaaatgtaa
     2401 tcaatatggt gtaaaaatat gtactaatac agaagctaca gtagaactta taaaggagaa
     2461 atcacctgat gtagttatta tagctactgg agcaactcca cttgtacttc ctataccagg
     2521 aatcaatgat tcaggattaa ttcatgctgt agaccttctt gatggtaaaa aatcttgtgg
     2581 gaaaaaagta cttgttgttg gaggtggaat ggttggatgt gaagtggcag ccttccttgg
     2641 agaacaagaa catgaggtta ctgttattga gcttagagaa gaagttggtg cagatgtaat
     2701 ttcagaacac cgtaaattcc ttatgaatga ttttgaaaat tataatatac ataccattac
     2761 tggtgcaaaa gtttctaaat tttatgatga tggtgtcgac tatttactta cagatggcac
     2821 agagcatgat ttaaaaggtt ttgattcagt agtacttgct atgggttctc gtaattacga
     2881 cccattaagt gaagttataa aagaagttgt aaaagaaact tatattgttg gtgatgcagt
     2941 aaaagctcgt agggcattag atgcaacaaa agaagctttt gaagtagcat taaatatata
     3001 aataataaat aaatttttct ttgtaaataa ctgaataggt tatttgcaaa gaaaatgata
     3061 atattgagag gagattatag ttatggaaat ttcaggtaga acaggattat ttgcattaat
     3121 aggtacaccg gttggtcact ctaaatcacc agtgatgtat aattatagtt ttaagaaatt
     3181 agatttagat tatagatatt tagcctttga tatcacagta gataaggtaa aagaggcact
     3241 tttagcaata aaaactttta atataaaagg agccaatgtt actatgccat gtaaatctgc
     3301 tgttacagaa tatatggatg aactttctcc agcagctcgt ataattggag catgtaatac
     3361 aattgtaaat gacaatggaa agttagttgg tcatataaca gatggagtag gctatgttcg
     3421 taatttaaaa gaaaatgggg tagaagttaa aggtaaaaag attacaatta tgggtgctgg
     3481 tggtgcagca actgctatac aagtacaatg tgctttagat ggagcaagag aaatatctat
     3541 ctttaatcct aaggatgatt tttataaaag agcagagcaa acagttgaga atataaaaaa
     3601 agatgtacca gaatgtgttg ttaatctata tgatttagaa gatacaaaca aactttatga
     3661 ggagatacaa tcaagtgata tattgacaaa tgcaactctt attggtatga agccttatga
     3721 caatgaaaca aatataaaag atactagtgt tttaagaaaa gatttagtag taacagatgt
     3781 cgtttataat cctaagaaaa caaaaatgat tgaagatgct gaagctaatg gatgtaaggc
     3841 tattggtgga ttaggaatgt tattatacca aggagcagaa gcttttaatc tatatactgg
     3901 attagaaatg cctgtagaag aagttaatga attatgcttt aaatagataa gagtaatatc
     3961 ttaaacaatg aaaaaacata cgtatttcaa tatttttaat aataaattac aaattgtcat
     4021 agtgggtttt gcctcatagt ccagttaaat ttgaagtttt aactgactat actttaaatc
     4081 cattatgggg atgagtttat tgtacacaaa aatgttaata aattaacaag aatgggagaa
     4141 aaattatgaa caataaaaaa tattatccta ctgccatagc gttgtatttt tcatattttt
     4201 tacttggtat tggaatttca attttaggac aatacaaacc agaattttca tcaatgtggg
     4261 gggctaaaac attatcagat ggaacattgg atataagtat tgttttagct gttattgctg
     4321 cactaggatt agggagactt atatcttatc catttgcagg acctatttca gataagtatg
     4381 gaagaaaagt aagtggtctt ataggaaact ttctacatgc aattttcttt gttggaattg
     4441 tattttcacc aaatttttat atagcatatg tatttgcaat aattggaggg gcagcaaatt
     4501 cattcttaga tacctgtgta actccttctt gtatggagat atttgctagt ttaggaacaa
     4561 tagcaaatat gtttactaaa tttacaatag ctttagcaca atttttgctt ccttttataa
     4621 ttggatttgt tgcagcaaat tcaatatcat ttaaagttat ttttatagta acagcgattc
     4681 ttattgtagt agatgccttt ttgatagctg tgttaccatt tccaccagct aataatgtta
     4741 ttgataacaa aggcaaaaca gttaaatcag agaagatgaa atttacacca acaagtatag
     4801 ctcttgtttg tattggattt acatgtacat ctacatttgt attatggtta aattgtaatc
     4861 aggaattggg taaattgtat gggatggctg accctacaaa aattcaatcc ttttactcaa
     4921 tgggagtaat atgtgcagta ttaataactt ctttacttat taaaaaatat attaaaccaa
     4981 taagaatatt agttatatat cctataatag ctttacttat gctattagtt gtatattttg
     5041 ttcaaactcc aactatttgt atgttagggg gatttgtaat aggttattca gcagcaggag
     5101 gtgttcttca gttaacaacg tcaactgcaa atgaaatgtt tccaactaat aaaggaaaaa
     5161 taacatctat agttatgatt gcatctagta tagcaaacta tgttatttta aatatagcag
     5221 gtattattac aaaatcaggg ggagttaatg gaccaaaata tgttgtattg tttaatgtgg
     5281 caataacatt tgttggaatt ttacttgcat tgtttgtaaa catgagatat gaaaaagaaa
     5341 aagtgtatga ttatgatgtc taatattagt ataatatatg catagagaag tctaaatcta
     5401 ggatagattt ttattaaaag taattaaaat taataaggat attatttaca gttataagat
     5461 agggtattta cataattaat aataaggata ttactagaat ttcaatttct agtaatattt
     5521 ttttgcgtat aaatgtaaac ttgatgaaat aagtagtata atcaatataa attacatcat
     5581 gtaaaggagg ataatttggt ggagaatgtt tttaagcgat tgcaggaatt taatggttat
     5641 gatggataca aagaatcatt tgaaatgaat tacctatgta tatatgaaag tataccgtta
     5701 agagaacaag tagagcttgc aaataatcta gtagatgaaa ttttaaatat gtataaaagt
     5761 gaatctaatg aaatttattt attggagggt agtaattcta agtctttaat ttgttatttc
     5821 gaaattttta tgaaaaagat aaatactttg gtaaaagaaa tgattattga tgagaaatgg
     5881 ttatataaat taactaaaga attaatatat aagagtaaaa aagttgagta tgtaaaacta
     5941 gggttagttt taagtgagaa atatctaaat gtagaaaact taagagaagt tgtagataca
     6001 ttttctaaat ctggagaata cgtattttat ctgagtaata ctattaaaaa acttgaattt
     6061 tacaatacat atttatttaa tttatctaaa aaggctacag gttcaataaa agtatttgct
     6121 attgttaata tggaaaattt agattctaaa ataaattcct atctaattga agatggttat
     6181 aaggatacaa agtacgaacg attattgatg aactatatta tttcaattgt agatttgaat
     6241 gaatatcttg aaaaacgaga tttagataaa gaaaagataa ataatttagc acgtcttata
     6301 tgcaattact tgctaagtgt agaatttaaa tatataggta ataaattaga attagtgaat
     6361 cgatttttac ctactgtagt aaattatggg actaactttg aaagtttata ttcaattttc
     6421 ctaatagcta taaacgtctt aaaagatgaa aatatagagt gcaataagat tgaatttgaa
     6481 aaagaaataa atggtatctt gctcagtgaa aaatggaaaa atatatattt tgaagcatta
     6541 agagatgcta gtggaaaaac agaagatatt ataaaaatgt cagaaattta tgatgtaaac
     6601 ttatcttttg atgacttgtt gccatatcta aatagagata ttagagattt tgaggtatat
     6661 tggcatatat ctaaaaaggg aacgacaagt tctagattaa aattattaaa tttttttgaa
     6721 gagacgttta aaatagatga tttaattgga aaaatgaaag atatagaaaa agataaattg
     6781 actcaagaat attatgatga tatgttattt tttattgtgc ttaaaggaag taaatcacta
     6841 tatcctgaag gaaagaatat atctttaaaa ggaatatttg gaaatataaa tgaagtaaga
     6901 aaagaatcca taaatatctt aaaaagatac agagagaaac tatctcttga agaattaaaa
     6961 attgttaaag aagcgtatga aaaagaaaaa aatgttatat tgaaggatga attaagaaga
     7021 gtattatatg aaagtaataa tttaaaaaaa gaatttgtaa atattgaaaa aataaaagta
     7081 gatgaacatg gtaaggatat ctatttgact tcaatagctg ttgctggttc aaggtttaga
     7141 aatagagaat atcttgaaaa agaattggag aaaagtaaga tatactattt aactagagag
     7201 aaagataatc tatatgatga aaaagctatt aaaatagttg gtgaaactgg atatgtgata
     7261 ggctatgtcc caagaaaaga aaactatatt ttgagcaatt tattagatgg aggaaaactg
     7321 ctgtattgta gagtaactga atataattta tatgaagatt gtatctacgc aaatgtgtac
     7381 ttgagttata aagatgtaat tgaaacagtt gaaaactctt taaagatggt tttagataag
     7441 agtagaataa aacttataaa ctaagacgtt tatatattgc ttagatgatg taatcctaac
     7501 tgattggtta ttttttctgg aattatggtg tattttatga gcaatgctaa tgaatataaa
     7561 ataaaaaaac aattgttttg tatatatgat gtatataaat atatttatat attcatattt
     7621 acaaaacaat tgaaatttat gttattttat aattttaaat ttagatggaa ttttaacttc
     7681 tgctttatct tttattttag aagcttgttt tttaacttta tttaataatt cagattcatc
     7741 tacaactaat acttttcttt ccttcatcaa ccatttacca tcacaaatag tagagtctac
     7801 attttcacta gtcatagaat aaactatatt tccaattgga ttatgcattg gtaaacaatg
     7861 aattgtatct ggatttagaa taatcatatc agcttttttt cctacttcaa gactaccaat
     7921 ttctttttct agtaaagcac attttgctcc atttatagta gccatttcta agacttcttc
     7981 agctggtacg acagttgggt ttaaagttct tcctttatgt attagtgatg taagatacat
     8041 gtccctcata aggtccattc tattatttga aggagctcca tcagtaccaa ttgaaacagg
     8101 tattcctttt tctaacattt caggaattga tgcaaaccct agaaccactt tcatagctga
     8161 acctgggtca tgagaaactt ttacatcatg taatctaaat aaatcaattt ccttatgagt
     8221 aagccaaact gtatgagcag ctaaaagatt tggtcctaat acacctagtt tatcaaggta
     8281 ttcaactgtt ccaactccat tgttttgctt tacaaatgaa atttcatcag ctatttcagc
     8341 aatatgcata tgtatacctg tattgtattt atcagctagt tctttagttc taacaagtaa
     8401 ttcttcagtt gtattaaaaa tagttcttaa tgcaaaccat attttaattc tctcattaga
     8461 tgtattatta aacttatgga ataagtctaa ttgagtgttg atttcctcat cagtagtttt
     8521 ttgccatgcc tcaggaagac catctcctgt atccataact gatttagcta aacaggctct
     8581 aagtccagtt ttatcaacag cttcaaccat agcatctaca tattgaccac cagattctaa
     8641 aaatgtagtg acaccagatt ttatcaattc aacacaacat gcagtagatg aaatcaagga
     8701 atcttcataa ttgaagctac tttcgtatgg ccaaattctt tctctaagcc aagttaaaag
     8761 tacaacatca tctgcaagac cacgtcctaa ttgttgagat aagtgaacat gagtattgac
     8821 tagccctggc attagtattt ttcctttagc atcatatatc tctgcatctt tatctacaat
     8881 attaatatca aacttaccaa cctcttttat aatattattt tcaataagta tgcttccatt
     8941 ttcatatacc tctctatctg cattcatagt aattagatat gcatttttta taagtaattg
     9001 tgacataaac accactcctt ttctatatat ttttatatct atatattatg ataataaatt
     9061 ttctatacaa agttaatagc aaaatcaatt tacatataaa gtgattttgc tatttataaa
     9121 tttaaaataa ataagatttt attctatata atttaaaata ttgacctaga actatttaaa
     9181 aatattaact tagaactaag tttacaactg caagtaaacc tataatccaa agtgtaatac
     9241 tgattttatc agtttcttta gctactacat gcattattat atatgaaata aatccaaagc
     9301 ataaaccaat acttatacta taagttaaag gcatcaataa aactgtaaca aaagcaggta
     9361 cagctatttt gctatcagca aaatcaatct gttttatgct ttcaaacatg aatacaccaa
     9421 ccattacaag tgcaggagct gcagcgtaag taggaactat accaagtaat ggtgtaaata
     9481 taagtgtagc taagaataat gctgctgtta ctattgaagc aagtcctgtt cttgcacctg
     9541 ctgcaatacc agaagcagat tctccaaatg ttgtaacagt actagttcct aagaatgagc
     9601 ctattaaagt agaagaaaca tctgcatata acattcttcc taaatctttt atttcaccat
     9661 tctcatcttc aagacccatt tgtttacaac aagtaataag aaatgctaat gaatcaaaca
     9721 tgtctataaa catgaaagag aatataggtc caattaatga gaatttcaat gcacctacaa
     9781 tatctaattt aaaagcaact ggtgctatac taggaggtgt cgagattatt gctgttggca
     9841 actccacaaa tccaaatata acagatatta ttgttgcaac tactatactt atcaatattc
     9901 cacctttaac ttttttatat tctagtattg ccattaagat aagagcaaaa agagctatta
     9961 aaactgttgg tgtaaaatct gcaagtgaaa ctatagtatc tggattagct attataacac
    10021 ccatattttt taaaccaaca aaagcaagga aaagacctat acctgctgaa gatgctactg
    10081 ccaattctct aggaattgcg tctgcaattg tctttcgaag acctgttact gcgagtgtaa
    10141 agaagacaca tccagataga aaaactaccc caagacctgt ttgccatggg attcctgcac
    10201 ctataaccaa agagtatgtg aagaatgcat ttaatcccat accaggagct agagcaaatg
    10261 gtgcatttcc taaaatcgca aatataagtg taccaatggc tgtagctagt atggtaactg
    10321 ttataagggc ggctttatcc attccagctt cagataatat tgctggattt acgaaaacta
    10381 tgtatgccat tgttaaaaat gtagtaactc cacctattac ctctgtttta aggctagagc
    10441 cacgttcgct aaatttaaac attttgtcta aaaagcttaa tgatgaatta tttccacctg
    10501 gttgtacatt gttagtgttt ggtaccattt ttacctccaa aaaactttaa gatataaaca
    10561 aaaaaatata tattcatata gatagaatag aacatatatt cagagttgac attctattca
    10621 tagaaaaccc atttaaggtg agtttgtaga aactattaga ccgtattact aatattatat
    10681 gaacattttc tatttgtttt tctgttgtat tatatcgtaa tattgtatta tttacaataa
    10741 atataataaa attattcgtt tttttgtcga ttttataaaa aattaaggtt atttattgaa
    10801 aaaaattact ttttccaata attgaat
//
LOCUS       DAELLV010000123        10712 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.123, whole genome shotgun sequence.
ACCESSION   DAELLV010000123 DAELLV010000000
VERSION     DAELLV010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10712
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.123"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            153..1097
                     /locus_tag="KPD58_002379"
     CDS             153..1097
                     /locus_tag="KPD58_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HBG0266141.1"
                     /translation="MIVSVSRRTDIPAFYSKWFFNRLKEGFVYVINPMNPKQVSKIEL
                     NPHTVDCFVFWTKDVTPMLYDLNKLKDYKYYFHYTITSYGKEVETCILDKRKVIDSFK
                     ELSRKIGKERVILRYDPIFLSEKYTVDYHIKAFASLCNQLDGFTEKCIISFIDLYKKT
                     KFNTKALHIKPIEITEIETLSKEISKIAHKHGIILEMCSEEYNLSKFGIGKSKCIDDR
                     LISKIIGCEVEVKKDSTQRDICGCVKSVDIGQYNTCRHYCLYCYANFNYAKVEENCRL
                     YKEDNKLLIDTLRDDAKISIRDMKTIKVDKNKQISIFD"
     gene            1266..2198
                     /locus_tag="KPD58_002380"
     CDS             1266..2198
                     /locus_tag="KPD58_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HBG0266142.1"
                     /translation="MNLLKKYPIPIASLILSIFTLGNLLQSYSESLRNVIGFIGFILY
                     AIYIVKIILLRKGVKEQLENPLISSTFPTITMATMIFATYIKPLSLELSIMIWSIGIV
                     GHLVLIVLFSKKFLVKFSIKTVFPSWYIVYVGIVAASVTAPAFNQLLIGKIAFWIGFV
                     SYIVLIPVILKRVWIIKEMPEPSLPSIAILAAPGSLLLSGYINSFSDKNNVVLYLLFI
                     LSIVFYIIVLGYLPKLLRLPFYPSFAAFTFPMAISAMGMKLMNGYLTKSGHFISWISY
                     ASKLEEIIATVIILYVLVLFIIYLSKSETTSKVK"
     gene            2303..2770
                     /locus_tag="KPD58_002381"
     CDS             2303..2770
                     /locus_tag="KPD58_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C_GCAxxG_C_C family protein"
                     /protein_id="HBG0266143.1"
                     /translation="MKTKEEYLKEVRNISEEYFRKGEFFCSEAVLQTINDALGQPLSP
                     EITKLASGFPIGLGKAQCLCGAISGGEMALGIVYGRVHGEAMNPKMFEHAKGLHDYIK
                     KEYGATCCRAITKKWDGDNFMSPERKEHCIKITGQVTEWVADKLIEDEKLNIK"
     gene            complement(3014..3868)
                     /locus_tag="KPD58_002382"
     CDS             complement(3014..3868)
                     /locus_tag="KPD58_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009912760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar uptake protein"
                     /protein_id="HBG0266144.1"
                     /translation="MLTIIIALLPVMGWGLMPIVANLKKSSPHEQLLGTSISAFIFST
                     IITLVIHPEITFFSFCISMISGIFWSLGQLMQFKAIQIASVSKVMPISNGSQLTFTTL
                     LAVILFNEWTSSKMLLIGSLSILCIILGILLTNYQTKKSTDKNMLKCVGVVLLSSLFL
                     SLYVVTNQIFLIEGYRIILPQSVGMLITSSIIVKYFSKEIVYMENVLFNLITGILWSI
                     ANLGMFITTNTLGVTISFSISQSCVIVATLGGIIFFKEKKNKKEWLAIFIGIILIMSG
                     VFMLSIIK"
     gene            4034..5998
                     /locus_tag="KPD58_002383"
     CDS             4034..5998
                     /locus_tag="KPD58_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG0266145.1"
                     /translation="MLTDMIQNREIKIIKFLLKEGHTTVKAIADDLDVSEKTVSKSLK
                     EVEAVLKKIGLSLIRKPKIGVYIDGDLKKILPYLDNKGSQIPTTREERVIYIYSVLLK
                     SNDYITIQQLSDELYISRGTIERDLAEVEKLLKNEGISIYKKPSKGIKLNISERDKRQ
                     LTAKFIHKFWSRNWYIKQEQESFYQAFEKIQADVNGIFSEDDLSIIIDILRNFSRERK
                     FEFSDYEFQSLVIHLAIAVERIRKGEYIEESLYTGNIENNQMSNTNYLAKQLEEKFSI
                     LLPKSEIGYINLHLVASDQHRDTNELKNTSTNPCETSTNNISNLREIIKNTLYETAYD
                     KDLIDGLTSHVHSSINRLKYGLSIKNPYVNTIKQNFSLSFEQALDLKKVIEFIYDIRI
                     NDDECAYIALHFEAFRERMKELPQNIRVILVCSTGLGSSRLLSARIKKYFPMIKIEKV
                     LSVQELVSSKIDADLVISTIYLELETIPTIVVSPILNESDIRTLERNIQNVSSYTSKK
                     YKSFRNLIFEENILLNIEASNYEDAIKYITDNLIKKGFAKEGIAKSAIERENLSYTSF
                     QSIATPHANPSYIKKSNISVATLKNPITWGNEKVEVIFFISLENDKNLELDEIYEVFY
                     DFIDNKKNINDILSATSTKEVYDLLVEESI"
     gene            5998..6459
                     /locus_tag="KPD58_002384"
     CDS             5998..6459
                     /locus_tag="KPD58_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0266146.1"
                     /translation="MKISDVLKKEQIVFNLDTNTKEETIKKLSSIFVENGIVDNEEEY
                     VKSVLEREEHSTTGIGNGIAIPHGKSNAVKKSSIIFAKTKHKIEWDALDEKPVDSIFL
                     LAISNIDKDSNHLVLLSKLATKLMDDDNVDALKLANSEQEIINIFSEEGEM"
     gene            6462..6782
                     /locus_tag="KPD58_002385"
     CDS             6462..6782
                     /locus_tag="KPD58_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBG0266147.1"
                     /translation="MKRKIIAVTACATGVAHTYMAAQALKKASKKMGHLIKVETQGAT
                     GIDNELTTKDAQIAEVVIFAVDTKVRNAERFEGKEILEVPVNAPIKDAEKVINDALKL
                     IDTK"
     gene            6798..7898
                     /locus_tag="KPD58_002386"
     CDS             6798..7898
                     /locus_tag="KPD58_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBG0266148.1"
                     /translation="MLKKIVSELKKHVLTGISYMIPLVIAGAMIMAISRVGGSFYNIP
                     DIWDAKYAESASSIVRLLHDLDGFGGTALGLMFPVIAAFIGFSIADKLAIVPGLVGGM
                     IAKDIGAGFLGALAVGLIAGYTCLFIKNHVKLPKSAASIVPIFIVPVFGTLITVVLIN
                     YVIGIPFATLNTGLENWLNGLSGTNQILMAAIIGAMMAVDLGGPVNKAALTTSLALLT
                     SGIYAPNTAAMVGIVIPPLGLGLATILAKQKYNKQLREAGKSSLIMGLIGVSEGAIPF
                     AVESPLKVIPSCVIGTAIASAMAVGLGSVNATPISGFYGWFTVENWPMYVLSIAVGTV
                     IVAGLVIVLRGNPVIEDDEFEDDDFDEAEWES"
     gene            8018..10618
                     /locus_tag="KPD58_002387"
     CDS             8018..10618
                     /locus_tag="KPD58_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBG0266149.1"
                     /translation="MIKAHIVNHTHWDREWYFTSGDALVLSEQLFTDVIDELERNPDV
                     SFVLDGQLSILDDYVQLHKEKIEIIKKLIKEDRLHIGPWFTQTDAFFARGESILRNLM
                     IGIFESKKYGKYMKIGYLPDTFGFNAQMPILLKHVGLDNIFFWRGIHLGKQVQSPYFK
                     WKSLNGESYIYAVNMPHGYGTGMLLEPSLKYVNGRLDPAIDFIKSYTDVDEVLIPSGN
                     DQLNIIGDFKNKIKAINDIGKYSYITSSYQEFLKYIKSIESLESYRGEFREPVLARIH
                     KSIGSSRMDIKLACDKLENKLIKRIEPLLVIAKKSKIEISNQLLINTWKKLLEGQAHD
                     SLAGCVTDTVTDDILHRIREANEICDSIENTIVKKISEGLKLSKNDILVFNTEAKRFN
                     GYKEIQVVSDSKNIYFKDNLDATIIEETYVKPRENVLEETPAGNIFIEEPGYYILKVR
                     INIQLPALGYKVVSFELSDKEMVSLDKSNDTFISNDNYKIIYEDGSLNLQLKDGTYIS
                     NFLTLKDSGNAGDTYDFSPLKNDEDIKLSFDKVYTEKALGYEKMVVKGTTLLPLTLED
                     RKNKYLKGKLNVKVSIVLSKENPLMDVKLCVDNTIYNHRLRVHIKTDIKDNKNIASLP
                     FGYITRKNGVLDNWEDIYSEMPIDLEPLESNITLTNQNRSCTVFTRGIKEYQHIEDEI
                     ALTLLATTDELGKPDLLYRPGRASGDTTKKGHIRIKTDKAQMLKELEFSFAIYMDSKS
                     FDELEIANLTHNYLRECVNYQLQDYNFFLYRIDNKIQKSIVNKTVSRELEIISLPENY
                     LISACYPSYYSKDKFIIRVENPSSNRMILDEQIFKNRNGKIVNAIEDVEEEQVYEIAP
                     FDVISILLDL"
BASE COUNT         4246 a         1181 c         1804 g         3481 t
ORIGIN      
        1 ttataacctt attaaggata ttttaaatgc tagaaatgaa tcagtaaatc atactggaat
       61 tatcttaaat gttttgatta aattaagtat ttagagctat aattatatta aaaatatatt
      121 agaacataat tatattacta tggaggttaa atatgatagt aagtgtgagt agaagaactg
      181 atatacctgc tttctatagt aagtggtttt ttaacagact taaagaaggt tttgtttatg
      241 taattaatcc aatgaatcca aaacaggtta gtaagataga gttaaatcca catactgttg
      301 attgttttgt attttggaca aaggatgtaa ctccaatgtt atatgattta aataaattaa
      361 aagactataa atattatttt cactatacta taacatcata tggaaaagaa gtagagacat
      421 gtattttaga taaaagaaag gtaatagatt catttaagga attgtctaga aaaataggca
      481 aagaaagagt aattttaaga tacgacccga ttttcttaag tgaaaagtac acagtagatt
      541 atcatatcaa agcatttgcg agtttatgta atcagcttga tggatttaca gaaaaatgta
      601 taataagttt tatcgatttg tataagaaaa caaaatttaa tactaaagca ttacatataa
      661 agccaataga aattacagaa atagaaacat tatcaaaaga gatttctaag attgctcata
      721 agcatgggat aatacttgaa atgtgttctg aagaatataa tttaagtaaa tttgggattg
      781 gaaaaagtaa gtgtattgat gatagattaa tatcaaaaat tataggatgt gaagtagagg
      841 taaaaaaaga tagtacacaa agagatattt gtggatgtgt taaaagtgtc gatataggac
      901 aatacaatac ttgcagacat tattgtctgt attgttatgc taactttaat tatgcaaaag
      961 tagaagaaaa ctgtaggctt tataaggaag ataataaatt actcatagat actttaagag
     1021 atgatgcaaa aataagcatt agagatatga agactattaa agtagataag aataaacaaa
     1081 ttagtatatt tgattaaaat aaatatacta attaccttaa tatattaaga atatagatac
     1141 ttttgataat aaaattaaac ttaatagata ttttctatat aagaaagatg gtcataatat
     1201 gtcaatttga ccatcttttt gatataatta aaacacaaat aatttagtaa caaaggaggc
     1261 agaacttgaa tttattaaaa aaatatccta tacctattgc aagtttgatt ttgtcaatct
     1321 ttactttagg taacttatta caatcttata gtgaatcatt acgtaatgta attggattta
     1381 taggatttat tttgtatgca atttatatag tcaaaataat attacttaga aagggtgtta
     1441 aagagcaatt agagaaccct ttaatatcaa gtacatttcc aactataaca atggcaacaa
     1501 tgatatttgc tacatatata aaaccattat ctttagaatt aagtattatg atttggagca
     1561 taggaattgt gggtcatctt gtgttaatag tcttatttag taagaagttc ttagttaaat
     1621 tctcaataaa aacggtattt ccatcttggt atatagtata tgttgggata gttgcagcta
     1681 gtgttacggc accagcattt aatcaacttt tgattggcaa aattgctttt tggataggat
     1741 ttgtatctta tatagttctt ataccagtaa ttttaaagag agtatggata atcaaggaaa
     1801 tgcctgaacc atctttacca agtatagcta tattagctgc accagggtca ctattattgt
     1861 ctggatatat taattctttt tcagataaaa ataatgtagt attatacttg ttattcatat
     1921 tatcaattgt attttatatt atagttttgg gatacttacc gaaattatta agattgcctt
     1981 tttatccgag ttttgctgca tttacattcc ctatggcaat aagtgcaatg ggtatgaaac
     2041 taatgaatgg ttatttaact aaatcaggtc attttataag ttggatttca tatgcttcta
     2101 agttagaaga gataattgct acagtgatta ttttatatgt tttagttttg tttattatat
     2161 atcttagtaa atctgaaact acatctaaag ttaaataaga attattaaat atgtgtattt
     2221 aataggaata ttgatataaa tatatgaaga taagataaac catagaaact taatttagct
     2281 taattttatt ggaggaaaac aaatgaaaac aaaagaagaa tatttaaaag aagttagaaa
     2341 catatcagaa gaatacttta gaaaaggaga gtttttttgc tctgaagcag ttctccagac
     2401 aataaatgat gctttaggcc aacctttaag tccagagata actaagttag catcaggatt
     2461 tccgatagga cttggaaagg cacaatgtct ttgcggagca atatctgggg gagagatggc
     2521 tttaggtata gtatatggaa gagttcatgg tgaagctatg aatccaaaga tgtttgagca
     2581 tgctaaaggt ttgcatgatt atattaaaaa agagtatggt gcaacatgtt gtagagctat
     2641 tacaaagaag tgggatggtg ataactttat gtctcccgaa agaaaagagc actgtattaa
     2701 aataacaggt caagttacag agtgggttgc agataagctt atagaagatg aaaaacttaa
     2761 tatcaaatag tgcaatatta gttggtgaat ttataaagtt aaatttactt atagtataag
     2821 atacttgata ttctcgtatt gatagaaaaa tttatagcat atgtatttaa tgaaatatac
     2881 ttatatttag agtgtggttt aatttcataa atacatatgc tattatttta agtatataag
     2941 caaatttatt taaataaata gaaacagtta atttatatgt acaaaactat atatgattag
     3001 tgtaaataaa tacctatttt ataatactca acataaagac accagacatg attagtatta
     3061 taccaataaa aatagcaagc cattcttttt tgttcttctt ttctttaaaa aatataatcc
     3121 ctcctaaagt ggctactatc acacaactct gagagataga aaaacttatt gtaactccca
     3181 atgtattagt cgttatgaac atacctaaat ttgctattga ccacaatata ccagtaatta
     3241 aattgaataa aacattttcc atatatacaa tctctttaga aaaatacttg acaataatag
     3301 atgaagttat caacatacca acagattgag gtaatataat tctatatcct tcaattaaaa
     3361 atatttgatt agttacaaca tataatgata aaaatagaga tgataataat acaactccaa
     3421 cacattttaa catattttta tctgtagatt ttttagtttg ataatttgtg agaagtatac
     3481 caagtattat acataatatt gataaagaac caatcagtaa cattttagaa ctggtccatt
     3541 cattaaacaa gattacagct aataatgtcg taaaagttaa ttgtgaacca tttgaaattg
     3601 gcattacttt agatacacta gcaatttgaa ttgctttaaa ctgcattaat tgtcccagac
     3661 tccagaatat gccagaaatc atacttatac aaaatgaaaa aaatgtaatt tcaggatgta
     3721 taaccagagt tattatagtg gaaaatataa acgcacttat ggatgtacct aaaagctgtt
     3781 catgaggaga acttttcttt agatttgcaa ctatgggcat caatccccat cccattacag
     3841 gaagtaaagc aataattatt gtcagcatta aactatttcc tttcatatta aatttagaca
     3901 gcttttaatt tatcatctaa aattaaagtg ttcaatacat aaaaattacc gtatttaatc
     3961 aggaaatatt agtagttttt tagaaaaaca ttttccatta aaataaagac aaagggaagg
     4021 agtaaaattg aaaatgttaa ctgatatgat tcaaaatcgt gaaattaaaa taataaagtt
     4081 tttattaaaa gaaggtcata caactgtaaa agcaatagca gatgatttag atgtttcaga
     4141 aaaaacagtt tcaaaatctc ttaaagaagt agaagcagtg cttaaaaaga taggtctttc
     4201 tttaataaga aaacctaaga taggtgttta tattgatgga gatttaaaaa aaatacttcc
     4261 ttacctagat aataaaggaa gtcaaattcc tacaaccaga gaggagagag ttatctatat
     4321 atattcagta cttttaaaga gtaatgatta tattactata caacaacttt ctgatgaact
     4381 atatataagt cgtggaacta ttgaaaggga tttagcagaa gttgaaaaac tgctaaaaaa
     4441 cgaagggatt tccatatata agaaacctag taagggaata aagttaaata taagtgaaag
     4501 agataaaaga caacttactg ctaagtttat acataaattt tggagtagaa actggtatat
     4561 taagcaagag caagaaagct tttatcaagc atttgaaaag atacaagcag atgtaaatgg
     4621 tattttttcg gaagatgatt tatcaataat tattgatatt cttaggaatt tcagcagaga
     4681 aagaaaattt gagttctctg attatgaatt tcagtcatta gttattcatt tggcaatagc
     4741 tgtagagaga attagaaaag gcgaatatat agaagaaagt ttgtatactg gtaatataga
     4801 aaataatcaa atgtcaaata ccaattattt agcaaaacaa ttagaagaaa aattttcaat
     4861 attattacca aaatctgaaa ttggatatat aaatttacat ttagtagcgt ctgaccaaca
     4921 tagagatact aatgaactaa aaaatacttc aactaatcct tgtgagacaa gtacaaataa
     4981 tatatcaaat cttcgggaaa ttataaaaaa tacattgtat gagacagctt atgacaagga
     5041 tttaattgat ggattaactt cacatgtaca ttcatcaata aatagactga aatatggact
     5101 tagtattaaa aatccatatg taaatacaat caaacaaaac ttttctttat catttgagca
     5161 agctttagat ttaaagaaag taattgaatt tatatatgat attaggataa atgatgatga
     5221 atgtgcttat atagcattac attttgaagc ttttagagaa agaatgaaag aacttccaca
     5281 aaatattaga gtaattctag tatgcagtac aggattaggt tcatctaggt tattgtctgc
     5341 aagaataaaa aaatactttc caatgataaa gattgaaaaa gtactttccg tacaagaatt
     5401 agtatccagt aaaattgatg cagatttagt aataagtact atttatttag agttagaaac
     5461 aattccaacc atagtagtta gtcctatttt aaatgaaagt gatataagaa ccttggaaag
     5521 aaatatacaa aatgtttcaa gttatacttc taagaagtat aaatctttca gaaacttaat
     5581 ctttgaagaa aatatattgt tgaatattga agcttcaaac tatgaagatg caattaagta
     5641 tataacagat aacctaataa aaaaaggttt tgcaaaagaa ggtatagcta aaagtgcaat
     5701 tgagagagaa aatttatctt acacttcttt ccaatcaata gctacacctc atgctaatcc
     5761 tagttatatt aaaaaatcta atatatctgt tgcaacgtta aaaaatccta ttacatgggg
     5821 aaatgaaaag gtggaagtta ttttctttat ttcattagaa aatgacaaaa acctagagct
     5881 tgatgaaata tatgaagttt tttatgactt cattgacaat aaaaaaaata taaatgacat
     5941 attaagtgca acttccacaa aggaagtgta cgatttgcta gtggaggaga gtatatagtg
     6001 aaaatatcag atgtattgaa aaaagagcaa attgttttta atcttgatac aaatacaaag
     6061 gaagaaacaa taaaaaaatt aagtagtata tttgttgaaa atgggattgt ggacaatgaa
     6121 gaagaatatg ttaagtctgt tttggaaaga gaagaacatt caactacagg tattggtaat
     6181 ggtattgcta ttcctcatgg aaaaagtaat gctgtcaaaa aatcttctat aatatttgca
     6241 aagactaagc ataagattga atgggatgct ttagatgaaa aaccagtaga tagtattttc
     6301 ttattggcta taagcaatat tgataaggat agcaatcatc ttgtactatt atctaaatta
     6361 gcaactaaac ttatggatga tgacaatgta gatgcactaa aattagccaa ttcagaacaa
     6421 gaaataatca atatatttag tgaagaagga gagatgtaga aatgaaaaga aaaattatag
     6481 cagttacagc atgtgcaaca ggagttgcac atacatatat ggcagctcaa gcgttaaaga
     6541 aagcttcaaa aaagatgggt caccttataa aagttgaaac acaaggagct acaggaatcg
     6601 ataatgagtt aactacaaag gatgcacaaa ttgcagaagt agtaatattt gcagtagata
     6661 ctaaagtcag aaatgctgaa cgttttgagg gaaaagaaat actagaagta ccagttaatg
     6721 cacctataaa ggatgcagag aaagtaataa atgatgcact aaagttaatt gacactaaat
     6781 aaatggaggg aaatataatg ttgaaaaaga tagtctctga gttaaaaaag catgtcttaa
     6841 ctggaatctc atatatgatt ccattagtaa ttgcaggtgc tatgataatg gcgatatctc
     6901 gtgtaggtgg ttctttctat aatataccag atatatggga tgctaaatat gcagaaagtg
     6961 ctagtagtat tgttagatta ttacatgatt tagatgggtt tggaggtact gcattaggat
     7021 taatgtttcc tgtaattgct gcatttatag gattttctat agctgataaa ttagctattg
     7081 taccaggtct tgttggaggt atgattgcta aagatatagg tgcaggattt ttaggagctt
     7141 tggcagttgg tcttatagca ggatatacat gtttgtttat taaaaatcat gtcaaattac
     7201 caaaatcagc agcttctata gtaccaatat ttatagtacc agtatttggg acattaatca
     7261 ctgttgtatt aatcaattat gtgataggta taccttttgc cacattaaat actggattag
     7321 aaaattggtt aaatggatta tctggtacaa atcagatatt aatggcagct attatagggg
     7381 ctatgatggc agttgactta ggtggaccag ttaataaagc agctttaaca acatcacttg
     7441 cattattaac aagtggaatt tatgcgccta acactgctgc aatggtagga atagttatac
     7501 caccactagg attgggactt gctactatac tagcaaaaca aaaatataat aaacagttac
     7561 gtgaagctgg gaaatcatct cttataatgg gtttaattgg agtaagtgaa ggagcaattc
     7621 cttttgcagt agaatctcca ttaaaagtaa taccttcatg tgtaattgga acagcaattg
     7681 cttcagctat ggcagttggt ttaggttcag taaatgcaac tccaattagt ggattttatg
     7741 gatggtttac agtagagaat tggccaatgt atgtgttatc aatagcagtt ggaacagtaa
     7801 ttgtagctgg attagttata gtattgcgtg gaaatccagt gattgaggat gatgaatttg
     7861 aagatgatga ttttgatgaa gcagagtggg aatcttaaaa tttaataact atttaagacg
     7921 tgttataagc tatattcaat atgaatctta tactaaataa agtgttaaat attcagttta
     7981 gacttaaata taaaagataa agaacagagg agaataaatg ataaaagcac atattgttaa
     8041 tcatacacac tgggatagag aatggtattt tacttctgga gatgcattag tacttagtga
     8101 acaactattt acagatgtaa ttgatgaatt agaacgtaat ccagatgtaa gttttgtact
     8161 tgatggacaa ctttctattt tggatgatta tgtacaatta cacaaagaaa aaatagaaat
     8221 cattaaaaag ttaataaaag aagatagact acatattgga ccatggttta ctcaaactga
     8281 tgcattcttt gctagaggag agtctatttt aagaaactta atgattggaa tttttgaaag
     8341 taaaaagtat ggtaagtata tgaaaattgg gtatttgcca gatacatttg gatttaatgc
     8401 acagatgcct atactattaa agcatgtagg tcttgataat atattctttt ggagaggtat
     8461 acatcttggt aaacaggttc aatcaccata ttttaagtgg aaaagcttaa atggagaaag
     8521 ttatatatat gcagtaaata tgccacatgg atatggaact ggtatgcttt tagagccttc
     8581 attaaagtat gttaatggac gcttagaccc agctattgat tttattaaat catatactga
     8641 tgtagatgag gtgttaatac cttctgggaa tgaccagtta aatataatag gtgatttcaa
     8701 aaataaaatt aaagcgatta atgatatcgg aaagtattca tatataacga gttcatatca
     8761 agagttttta aaatatataa agtccataga atcactagaa agctatagag gtgaatttag
     8821 agaacctgta ttagcaagaa tacataaatc aattggttct agtagaatgg atataaaatt
     8881 agcatgtgat aaactagaaa acaagctgat taaaagaata gagccattat tagtaattgc
     8941 taaaaagagt aaaattgaaa taagcaatca gttacttata aatacatgga aaaaactttt
     9001 ggaaggacaa gcacacgata gtttagctgg atgtgtaact gatactgtaa cagatgatat
     9061 tcttcaccgt ataagagaag caaatgaaat atgtgacagt attgaaaata ctattgtaaa
     9121 aaagatttca gaagggctta aattatctaa aaatgatatt ttagtattta atacagaagc
     9181 taagcgtttt aatggatata aagagataca agttgtaagt gatagtaaaa atatttattt
     9241 taaagataat ttagatgcta ctataattga agaaacttat gtaaaaccga gagaaaatgt
     9301 attagaagaa acacctgctg gtaatatatt tattgaagaa ccaggatatt atatattaaa
     9361 agtaagaata aatatacagc ttcctgcact tggttataaa gttgttagtt ttgaattaag
     9421 tgataaagaa atggtgtcac ttgataaatc taatgacact tttatatcta atgataatta
     9481 taagataata tatgaagatg gaagcttaaa tttacaactt aaagatggaa cttacatatc
     9541 aaacttttta acattaaaag atagtggtaa tgcaggtgat acctatgact tttctccact
     9601 aaaaaatgat gaggatataa aactttcgtt tgataaagtg tatacagaaa aagctttagg
     9661 atatgaaaaa atggtggtta aaggtactac tttactacca cttacattag aagatagaaa
     9721 aaacaaatat cttaaaggga aattaaatgt aaaagtaagt attgtcttat ctaaagaaaa
     9781 cccattgatg gatgtaaaac tttgtgttga taacacgata tataatcata gactacgtgt
     9841 tcatataaaa actgatataa aagataataa aaacatagca tcattgccat ttggatatat
     9901 aacaagaaaa aatggagttt tagacaactg ggaagatatt tacagtgaaa tgcctataga
     9961 tttagaacct ttggaaagta atattactct aacaaaccaa aatagaagtt gtactgtatt
    10021 tactagagga ataaaagagt atcaacatat tgaagatgaa attgcattga ccttacttgc
    10081 tactacagat gaactaggaa agcctgattt attatataga ccaggacgtg cttctggtga
    10141 tacaactaaa aaaggtcata taagaattaa aacagataaa gctcaaatgc ttaaggaatt
    10201 agaattttca tttgcaatat atatggattc taaatcattt gatgaattgg aaattgcgaa
    10261 tttaacccac aattatctaa gggaatgtgt aaattatcaa ttacaggatt acaatttctt
    10321 tttatatcgt atagataata agattcaaaa atcaattgtc aataaaactg taagtagaga
    10381 attagaaata atatctttac cagaaaacta tcttatatca gcatgttatc cttcttatta
    10441 cagtaaagat aaatttataa taagagtgga aaatccaagt agtaatagaa tgatattgga
    10501 tgagcaaata tttaaaaata gaaatgggaa aattgtaaat gcaatagaag atgtagaaga
    10561 ggaacaggta tatgaaatag caccttttga cgttatttca atattattgg atttataagt
    10621 gtaaaagtaa gtttattgtt taaagtctga cagatattaa tctgccagac tttttaattt
    10681 gtataaattt gggtaaatat taatgaatgg tt
//
LOCUS       DAELLV010000124        10698 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.124, whole genome shotgun sequence.
ACCESSION   DAELLV010000124 DAELLV010000000
VERSION     DAELLV010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10698
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.124"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            123..587
                     /locus_tag="KPD58_002388"
     CDS             123..587
                     /locus_tag="KPD58_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HBG0266150.1"
                     /translation="MATMTDIIEKFIKDLMEEDNSIQIQRNELANLFSCAPSQINYVL
                     TTRFTIDRGYYIESKKGGGGYVQIEKIRKSKDGHIRELLNEKIGSQISYKKAKELLEG
                     LKESDLINERELKLILYAIDDKSLCMPIYELKEKVRSNILKNIIIGLFSIEE"
     gene            594..1088
                     /locus_tag="KPD58_002389"
     CDS             594..1088
                     /locus_tag="KPD58_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HBG0266151.1"
                     /translation="MLCQKCNKNKASVYYNKIVNGEKTEMYLCSECAKENTEMNFNLD
                     MPFSMMDIFSNLGFQPKKELEEKLVCPKCNTTYSEFKNNGRFGCSECYNAFSSQVNPM
                     LQNIHGHIEHTGKAPKKSFYKISVENEIKELKEDLDRAIKNEEYELAAQFRDKIKYLK
                     GSID"
     gene            1104..2129
                     /locus_tag="KPD58_002390"
     CDS             1104..2129
                     /locus_tag="KPD58_002390"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HBG0266152.1"
                     /translation="MKENIVMKSRVRLARNLNNYPFPNKLDKECAMEIIEKVKNAFIN
                     SNLEQKEEFDFYKIEDLDQSKKMLMVEEHIISPDLAENDKSAVIVKKDKTISIMINEE
                     DHIRIQTICDDLNLEYAYSVANEIDDLLESSLEYAFNTKLGYLTSCPTNTGTGMRASV
                     MMHLPALSQLGYMDELYKISSQIGIAIRGIYGERTEALGNIYQISNQLTLGRTESNII
                     ENVSGLTKDAISKEIKAREILQKKLGIKLEDKIFRSIGTLENSRVMSSAEAMSHLSNI
                     KMGIEMNYIDKLDLRAIEQLMIGIQPAHQSIMYKSDDVENRDINRATYIRETLEKLRG
                     GMNCELQ"
     gene            2116..4563
                     /locus_tag="KPD58_002391"
     CDS             2116..4563
                     /locus_tag="KPD58_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBG0266153.1"
                     /translation="MNFNRFTQRAKKAIDLAFESAKSLGHNIVGSEHILLGLLREEEG
                     IAAKVLSKVGFTEAYLEGKIVDMEGKGEEISEDIVLSPRSKQILELSGMFANKLKTNY
                     IGTEHILLAIIQEGEGIANKILNYAGVNDRTLAQLTIDMMGISDKNQYKAENSYTGNQ
                     NQAESKVLDKYGRNLTLYAKQNKIDPVIGREKEIQRVIQILSRRTKNNPVLIGDPGVG
                     KTAIAEGLATNIALGNVPETLKNKTLYSLEMGSLLAGAKYRGEFEERIKEVVDEVVKN
                     GNIILFIDEMHTIIGAGSTGEGSIDASNILKPALARGEIQVIGATTIDEYRKHVEKDS
                     ALERRFQPVMVDEPTKEDSIKILEGLRDKYEAHHKVKITDDAIKAAVELSTRYISDRY
                     LPDKAIDLIDEAASKVRLKENTPPSEIKKLELEIENIDKEKEEAVRCQDFEKAAKIRD
                     EQGLLKKQLEDVRERWNKSSKHSDLVDGEVIAEVVGLWTGIPVNKILEEEADRLLRLE
                     EILHNRVIGQEQAVKSISKAIRRSRAGLKDPNRPIGSFLFLGPTGVGKTELSKALAEV
                     QFGDENQIIRIDMSEYMEKHAVSRMIGSPPGYVGHDEGGQLTEKVRRNPYSVILFDEI
                     EKAHPDVFNILLQILDDGRLTDSKGRTVDFKNTIVIMTSNVGASTIGRQKTLGFSIAK
                     GDEEEKSQYEKMKENIMGELKQRFRPEFLNRIDDIIVFHSLNENHISKIVLLMAAKLQ
                     ERLKEMDIKLEMSDEAVKLISKSGFDLEYGARPLKRALQKELEDELSEAILKGNVKKG
                     SNVVAKVKDEKIVFETK"
     gene            4743..6116
                     /gene="radA"
                     /locus_tag="KPD58_002392"
     CDS             4743..6116
                     /gene="radA"
                     /locus_tag="KPD58_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBG0266154.1"
                     /translation="MAKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKK
                     EVFIIDKSSSKPVSINSIESKEEERFTTDINELDRVLGGGIVKGSLVLVGGDPGIGKS
                     TLLIQVSSNVANLGKTVLYITGEESESQIKMRAKRLGINSENLYIFAENNLSIIESYL
                     ESVNPELIILDSIQTVFSPEISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKE
                     GSLAGPKLLEHMVDTVLYFEGERYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDN
                     PSKILISEKPKDVAGSVIISTVEGTRPMLLELQALVSPTSFGIPKRTSTGVDYNRVGM
                     LLAVLEKRVGLQIQNQDVYINIVGGIKINEPSIDLGIAISVASSFRNIPIDEDIAVTG
                     EVGLTGEVRAVSFIEKRIAECKKLGFKKIVVPRSNYDVVKETKGIEIWPVDNLRQAIN
                     IVLGRNQ"
     gene            6119..7189
                     /gene="disA"
                     /locus_tag="KPD58_002393"
     CDS             6119..7189
                     /gene="disA"
                     /locus_tag="KPD58_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA"
                     /protein_id="HBG0266155.1"
                     /translation="MENFLDNKNMLYALKMISPGTPLRLGLNNVLRAKTGGLIVIATN
                     EDVMKIVDGGFAINAEYSPSYLYELAKMDGAIVLSGDVKKILFANAQLIPDYFIETSE
                     TGTRHRTAERVAKQTGAIVIGISQRRNVITVYRGNEKYVVEDISKIFTKANQAIQTLE
                     KYKTVLDQAVTNLNALEFNDLVTIYDVALVMQKMEMVMRVTSIIEKYVIELGDEGTLV
                     SMQLEELMGTTRIDQKLIFKDYNKENTEIKELMKKVKNLNSEELIELVNMAKLLGYSG
                     FSESMDMPIKTRGYRILSKIHRLPTAIIENLVNYFENFQQILDASIEELDEVEGIGEI
                     RATYIKNGLIKMKQLVLLDRHI"
     gene            7278..8372
                     /locus_tag="KPD58_002394"
     CDS             7278..8372
                     /locus_tag="KPD58_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HBG0266156.1"
                     /translation="MIRKVTRILFILLGFTIGITTYLTLMKDFEILTFGKETYGYIAA
                     VVAGIIIAILGYLIEPWVVNKVKEIAKIVDKELSKYPQTDILLGSMGLIVGFVIAYLL
                     SGLVNRIPIVGGILSLLLYLFLGYLGMKVALKSKNDLFNIGKLGRLANPIKDKDKDKE
                     NKKEVKAIPPKVLDTSVIIDGRIADICKTGFIEGKLIIPAFVLEELRHIADSSDDLKR
                     VRGRRGLDILNIIQKELNIEVEISERDFDDIAEVDSKLLKLAQVLNGKVVTNDYNLNK
                     VAQFQGVEVLNINELANAIKPVAIPGEDMVVQVVKEGKEAQQGVAYLDDGTMIVVDGG
                     RKYMNETIKVLVTSVLQTPAGRMIFAKPKN"
     gene            8501..10678
                     /locus_tag="KPD58_002395"
     CDS             8501..10678
                     /locus_tag="KPD58_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266157.1"
                     /translation="MRKKIISFLATFIIILTSASQYSFADDISTRGKVIFIDMNRTSM
                     SNMLRIKSLREELDNRGYIGLMNIRGDKGSDDRRSYASMGAGGRANVANEEDINFESS
                     SKDRNIVFESATGKSAKGINNLTINKSINENLNFGEYGSVLGSLGQSLSDNGLKASVL
                     GNSDIIENGQLIKNRNLCLTAMDEYGRIPNGNVDTINKKDLSMPYGISTDYDKLIVET
                     KEAYKNNDVIFVELGDTYRLDLYKPNLNEKTYESMKDSIEKNIDIYLKNIFSMVEEND
                     TVYIASAFPSDLDYKNKRRLSPIIKLNGEGKGILSSSTTRREGIVTNLDVGVEILDNF
                     NIKNQNMVGRKYELINRDDNKEFLMDEYQKIVSISSIRSTILNGFVSIVFLSWIVAMI
                     AILFRKHISKNYKETTFFILKELLKIGIVMPLSFMITPIMNFKTPLAISLGIIIITLT
                     IYLISKVLIKNDLKNMLFFTGLTIVIMVIDAGFGSYLMKSNVMSYDCIIGARYYGVGN
                     EYQGVAIGSAIFTFAILLTYKNIPKWSIIVFSLVILITSASPIMGANVGSAISECVAF
                     LLFILLIYNVKIDFKKIVLLGIAVVFVLGVFVAIDMILGSNSHLGMFVKEIYFNGPGE
                     IIQTFSRKIEMNLKLAQTSAWVNILLTGIGVILVLMINQIRYFKQLMDEYPIVFKGFI
                     ASIAGCLVTLLVNDSGIVSSATAFIYVIVPMITLSINLTALKE"
BASE COUNT         4457 a         1088 c         1996 g         3157 t
ORIGIN      
        1 ttcatatcta aatttctata aaaaatttaa atttatacaa tttagggctt taaaaattta
       61 aataataggt gtaaaataat agtcaaagaa agtcaaaaat aaaaaggtgt gtggtgatat
      121 taatggcaac tatgacagat ataattgaaa aatttattaa agatttgatg gaagaagata
      181 atagcataca aattcaaaga aatgaactag caaacttgtt tagttgtgca ccatcacaaa
      241 taaattatgt cttaacaaca agatttacaa tagatagagg atattacatt gaaagtaaaa
      301 aaggcggcgg aggatatgtt caaatagaaa aaatacgaaa aagtaaagat gggcatatta
      361 gagaactact aaatgaaaaa ataggaagtc agatatccta taaaaaagca aaagaattat
      421 tagaaggttt aaaagagtcg gatttgatta acgaaagaga attaaaatta atattatatg
      481 caatagatga taaatcattg tgtatgccaa tatatgaatt aaaagagaag gtaaggtcta
      541 atattttaaa gaatataata ataggattat ttagtataga ggagtgataa tttatgctat
      601 gtcaaaaatg caataagaat aaagcatctg tatattataa taagatagtc aatggagaaa
      661 aaacagagat gtatttatgt agtgagtgtg caaaagagaa tacagaaatg aattttaact
      721 tagatatgcc tttttcaatg atggatatat tttctaattt aggttttcaa cctaaaaaag
      781 aacttgagga aaagctagtt tgtcctaaat gcaatacaac atatagtgag tttaagaata
      841 atgggagatt tggatgtagt gaatgttata atgcatttag tagtcaagta aatccaatgc
      901 ttcaaaatat acatgggcat atagaacata caggaaaagc acctaaaaaa tctttttata
      961 agataagtgt agaaaatgag attaaagaac taaaagaaga tttagataga gctataaaaa
     1021 atgaagagta tgagttagca gctcaattta gagataaaat taaatattta aaaggttcta
     1081 ttgactaaga ttggagtgat attatgaaag agaacatagt catgaaatca agagttaggc
     1141 tagctagaaa tttaaataac tatccatttc caaataaatt agataaagaa tgtgccatgg
     1201 aaataataga aaaagtaaaa aatgcgttta ttaattctaa cttagaacaa aaagaagaat
     1261 ttgatttcta taaaattgaa gatttagacc aatctaaaaa aatgcttatg gttgaagaac
     1321 atataataag cccagattta gcagaaaatg ataaaagtgc agttatagta aaaaaagata
     1381 aaactataag tataatgata aatgaagaag accatataag gatacaaact atatgtgatg
     1441 atttaaactt agaatatgca tatagtgtag caaatgaaat agatgatttg ttagaatcaa
     1501 gtttagaata tgcctttaac acaaagctag gttatctaac atcttgtcca actaatacag
     1561 gaacaggaat gagagcttct gtaatgatgc atttaccagc attaagtcaa ctaggctata
     1621 tggatgaact ttataaaatt tcatcacaaa taggcatagc aattagaggg atatatggag
     1681 aacgaacaga agcattggga aatatatatc aaatatctaa tcaacttaca cttggaagaa
     1741 cagagtctaa tattatagaa aatgtaagtg gacttacaaa agatgcaatt tcaaaagaaa
     1801 taaaagctag agaaatattg caaaaaaaat taggtataaa actagaagat aaaatattta
     1861 gatcgatagg gacacttgaa aattcaagag taatgagtag cgcagaggca atgagccatc
     1921 tatcaaatat aaaaatggga atagaaatga actatataga taaactagat ttaagggcta
     1981 tagaacaact tatgataggt atacaaccag cgcatcagtc aataatgtat aagagtgatg
     2041 atgttgagaa tagagatata aatagagcaa catatattag agaaactttg gaaaaattaa
     2101 gaggagggat gaattgtgaa cttcaataga tttacacaaa gagctaaaaa ggcaattgat
     2161 ttagcgtttg aatctgctaa aagtctagga cacaatattg tcggaagtga acatatactt
     2221 ttaggtcttt taagagaaga agaaggtata gctgctaaag ttctaagtaa agttggattt
     2281 acagaagctt atttagaagg caaaatagtt gatatggaag gtaagggaga agaaatctca
     2341 gaagatatag tattaagtcc aaggagtaaa caaatacttg aattatcagg aatgtttgca
     2401 aataaattaa aaacaaatta tattgggact gagcatatat tattagcaat tattcaagaa
     2461 ggtgaaggaa tagctaataa gattttaaat tatgctggag taaatgatag gactttagcc
     2521 caattaacaa ttgatatgat gggtattagt gataaaaatc aatacaaagc agagaatagt
     2581 tacacgggca accagaatca agcagaatct aaagtacttg ataagtatgg aagaaatctt
     2641 acactatatg caaaacagaa taaaatagac cctgtaatag gtagagaaaa agaaatacaa
     2701 agagtaatac aaatattaag tagaagaact aaaaacaatc cagtgttgat aggagaccca
     2761 ggtgtaggga aaacagccat agctgaaggt ctggctacaa atatagcctt aggaaatgtt
     2821 ccagaaacac taaaaaacaa aactttatac tctttagaga tgggttcatt attggctgga
     2881 gctaagtata gaggagaatt tgaagaaaga attaaagaag ttgtagatga agttgttaaa
     2941 aatggaaaca taatcttatt tatagatgag atgcacacaa taataggggc tgggtctact
     3001 ggagaaggct ctatagatgc ttcaaatata ttaaagccag ccttagcaag aggagaaata
     3061 caagttatag gtgcaactac aattgatgaa tatagaaagc atgttgaaaa agattctgct
     3121 ctagaaagaa gattccaacc agttatggta gatgaaccaa ctaaagagga ctcaataaaa
     3181 atattagaag gattaagaga taaatatgaa gctcatcata aagttaaaat aactgatgat
     3241 gctataaagg cagctgtaga attatcaact agatatatat cagacagata tctgccagat
     3301 aaagctatag acttgattga tgaagctgca tcaaaagtaa gattaaaaga aaatacacct
     3361 ccttcagaaa taaaaaaatt agaactagaa atagaaaata tagataaaga aaaagaagaa
     3421 gcagtaagat gtcaagactt tgagaaagca gccaaaataa gagatgaaca aggtctactt
     3481 aaaaaacaat tagaagatgt tagagaaaga tggaacaagt catctaaaca ttcagattta
     3541 gttgatggag aagttattgc tgaagtagtg ggcttatgga caggaatacc agttaataaa
     3601 attcttgaag aagaggctga tagacttttg agacttgagg aaatactgca caatagagtt
     3661 ataggtcaag aacaagcagt aaaatctatt tcaaaggcaa tcagaaggtc aagagcaggt
     3721 cttaaagacc ctaatagacc aattggttca tttttatttt taggtccaac aggagtaggt
     3781 aaaacagaat tatctaaggc attagcagaa gtgcaatttg gagatgaaaa tcaaataatt
     3841 agaatagata tgtctgaata tatggaaaaa catgctgtgt caagaatgat aggttcacct
     3901 ccaggttatg taggtcatga tgaaggtggt caattaactg aaaaagtaag aagaaatcca
     3961 tattcagtta tcttatttga tgagatagaa aaagctcatc cagatgtatt taatattctg
     4021 ttacaaattc tagatgatgg tagactaact gattcaaaag gaagaactgt agattttaag
     4081 aatactattg taataatgac atcaaatgtt ggagcatcta caattggtag acaaaaaact
     4141 ttaggattta gtatagctaa aggagacgaa gaagaaaaat ctcaatatga aaaaatgaaa
     4201 gaaaatataa tgggtgaatt aaaacaaaga ttcagaccag agtttttaaa cagaatagat
     4261 gatataattg ttttccactc actaaatgaa aatcatatat caaaaatagt acttttaatg
     4321 gcagcgaaac tacaagagag attaaaagaa atggatataa aattagaaat gagtgatgag
     4381 gctgttaaat taatttctaa atctggattt gacttagaat atggagcaag acctcttaaa
     4441 agagctttac aaaaagagtt ggaagatgag ctatctgaag caatcttaaa aggtaatgtg
     4501 aaaaagggaa gtaatgtagt agcaaaagtc aaagatgaaa aaatagtttt tgaaactaaa
     4561 taatttaata gagttgcatt ttaaaatatt gcaaatataa gttttataat ctaaacaaaa
     4621 ttttaagggc aatgaatcta taataattca ttgtccttaa ttattttatt taatctttac
     4681 atattgtctt tcagatatgc taaaattata gtatagaata aaaaattaag gatgataaaa
     4741 gtatggcaaa aataaaaaca aaatatgtat gtcaatcttg cggatatgaa acagctaaat
     4801 ggcttggtaa atgtccagaa tgtacaaaat ggaatacatt tgtagaagaa atagaccaaa
     4861 aaagcaccaa aaaagaggtc tttataatag ataaatcttc atctaaacca gtcagtataa
     4921 actcaataga gagtaaagaa gaagagcggt ttacaacaga tataaatgaa ttagatagag
     4981 tgcttggagg aggtatagtt aaaggctctt tagtgcttgt tggtggtgac cctggtatag
     5041 gtaaatcaac acttttaatc caagtatcta gtaatgttgc caatttagga aaaactgttt
     5101 tatatataac tggagaagaa tcagaatctc aaataaaaat gagagctaaa agattaggta
     5161 ttaactcaga aaatttatat atatttgctg aaaataattt aagtataatt gaatcttatt
     5221 tagaaagtgt aaatccagaa ttaataattc tagactcaat tcaaactgtc ttcagtccag
     5281 aaatatcctc ggcaccagga actgtcagcc aaattaaaga aggtacttca aaattcatga
     5341 aaatttctaa aaaaatggga atttcaacgt tcatagttgg gcatgttaca aaagaaggtt
     5401 cactagcagg accaaaatta ttagagcata tggttgatac tgttctatat tttgaaggag
     5461 aaagatataa tacttataga ttagtaagag ctgttaaaaa tcgatttggt tcaactaatg
     5521 aacttggagt gtttgaaatg agggatttag gattagtaga acttgataat ccatctaaaa
     5581 ttttaatctc tgaaaaacca aaagatgtag caggttcagt aataatatct actgtagaag
     5641 gtacgagacc aatgttactt gaattgcaag cattagtttc tcctacaagt tttggtatac
     5701 caaaaagaac atctacagga gtcgattaca atagagtagg tatgttatta gcagtattag
     5761 aaaaacgtgt tggtttgcaa atacaaaatc aagatgtcta tataaacata gttggaggaa
     5821 taaagataaa tgaaccctca attgatttag ggatagccat ttctgtagca tctagtttta
     5881 gaaatatacc gatagatgaa gatatagctg ttacaggaga agtgggactt acaggagaag
     5941 ttagagcagt aagctttata gaaaaaagaa tagctgagtg taaaaaactt ggatttaaaa
     6001 agatagtagt tcctagaagt aattatgatg ttgtaaaaga gaccaaaggc atagaaatat
     6061 ggccagtaga taacttaaga caagctataa atatagtact tgggaggaat caataaatat
     6121 ggagaatttt ctagataata aaaatatgct atatgcatta aaaatgatat ctcctggaac
     6181 tccacttaga ttaggtctaa acaatgtact aagagctaag actggtggat taattgtaat
     6241 tgcaacaaac gaagatgtaa tgaaaatagt agatggagga tttgctataa atgcagaata
     6301 ttcaccatca tatctatatg aattagctaa aatggatgga gctatagttt taagtggtga
     6361 tgtaaagaaa atattatttg ctaatgcaca acttatacct gactatttta tagaaacatc
     6421 agagacagga acaagacata gaacagcaga aagagtagca aaacaaactg gtgctatagt
     6481 cataggaatt tcacaaagaa gaaatgttat aacagtttat agaggaaatg agaagtatgt
     6541 agtcgaagat atatctaaga tatttactaa ggcaaatcag gctatacaaa ctctggaaaa
     6601 atataagaca gtattggacc aagctgtaac aaatttaaat gccttagagt ttaatgattt
     6661 ggtaactatt tatgatgttg cattagtcat gcaaaagatg gaaatggtaa tgagagttac
     6721 aagtataatt gaaaaatatg tgatagaatt gggtgatgaa ggaactttag taagtatgca
     6781 attagaagaa ttaatgggta caaccagaat agaccagaaa ttaatattca aagattataa
     6841 taaagaaaac acagaaataa aagaacttat gaaaaaggtc aaaaatttaa attcagaaga
     6901 actaatagaa ttggttaata tggcaaaact attagggtat agtggttttt cagaaagtat
     6961 ggatatgcct ataaaaacaa gaggttatag gattcttagc aaaatacata gactaccaac
     7021 agcaataata gaaaacttag taaattattt tgaaaacttt caacaaattt tagatgcatc
     7081 tattgaagaa ttagatgagg ttgaaggaat aggtgaaata agagcaacat atataaaaaa
     7141 tggactcata aaaatgaaac aattagtctt attagataga cacatatgat gaattttaaa
     7201 tgaattttac tacttttttc ttgaatataa tatacacttt ggtctataat agataagtat
     7261 aactaggggg tgaagcattg ataaggaaag taacaagaat attgtttatt ttacttggat
     7321 ttacaatagg tataacaacc tatttaacat taatgaaaga ttttgaaata ttaacatttg
     7381 gaaaagaaac atatggttat atcgcagctg tagtagcagg tataataata gccattttag
     7441 gatatcttat agaaccatgg gttgtaaata aagtaaaaga aattgctaaa atagtggata
     7501 aagaattgtc aaaatatcct cagactgata tattgttagg gtctatgggt cttatagtag
     7561 gatttgttat tgcgtattta ttaagtggat tagtaaatag aataccaata gtaggtggaa
     7621 tactatcctt actattatat ttattcctag gatatcttgg aatgaaagtt gcgcttaaaa
     7681 gtaaaaatga tttatttaat attggaaaac taggaagatt agcaaatcct attaaggata
     7741 aagataagga taaagaaaat aaaaaagaag taaaagccat tcctccaaaa gtattagata
     7801 caagtgtaat aattgatgga agaatagctg atatttgtaa gactgggttt atagaaggaa
     7861 aacttattat tcctgctttt gttttggagg aactaagaca tattgcagat tcttcagatg
     7921 atttaaaaag agtaagaggt agaagaggtc tggatatatt aaatataatt cagaaagaat
     7981 taaatataga agttgaaata agtgagagag actttgatga tatagcagaa gttgatagta
     8041 aattattaaa gcttgctcag gttttaaatg gaaaagttgt tacaaatgat tataacttaa
     8101 ataaagttgc tcaattccaa ggtgttgaag tattaaatat aaatgagtta gctaatgcta
     8161 taaaaccagt tgctatacca ggtgaagata tggttgttca agtggttaaa gaaggaaaag
     8221 aggctcaaca aggtgttgca tatctagatg atggaactat gatagttgtt gatggaggaa
     8281 gaaaatatat gaatgagact ataaaagtac tcgttacttc tgttcttcaa actcctgcag
     8341 gtagaatgat atttgcaaaa cctaaaaatt aatatgttca aagaaaccta taatttgggc
     8401 tgtttatttt aaatagatgg tccaaattta tttatttata acgataatgt ttaatgtatt
     8461 cttaaagatg gggatttaaa tcagaaagca gggagaattt atgagaaaaa agattatatc
     8521 tttcttagca acatttatca taatattaac atcagcaagt cagtatagtt ttgcagatga
     8581 tatatcaaca cgtggaaaag taatatttat agatatgaat agaactagta tgagtaatat
     8641 gttaagaata aaatcattaa gagaagaact agacaataga gggtatattg gcttgatgaa
     8701 tataagaggc gataaaggaa gtgatgatag aaggtcatat gctagtatgg gtgctggtgg
     8761 tagagctaat gtggctaatg aagaagatat taattttgaa agctcgagta aggatagaaa
     8821 tatagttttt gaatcagcta caggaaaaag tgcaaaaggt ataaataatt taactataaa
     8881 taagtctata aatgaaaatt taaactttgg agagtatggt tctgtattag ggtcattagg
     8941 acaaagttta tctgataatg gattaaaagc atctgtattg ggtaattcag atataattga
     9001 aaatggtcaa cttataaaaa atagaaactt atgtctaact gcaatggatg aatatggaag
     9061 aatacctaat ggaaatgtag atactataaa taaaaaagac ttatctatgc cttatggaat
     9121 atcaactgat tatgataaat tgattgtaga gactaaagaa gcttacaaaa ataatgatgt
     9181 gatttttgtg gagcttggag acacatatag attagattta tataaaccaa atttaaatga
     9241 aaaaacatat gagagtatga aagatagcat agaaaaaaat atagatatat atttaaaaaa
     9301 tatattttct atggttgaag aaaatgacac tgtctacata gcaagtgcat ttccaagtga
     9361 tttagattat aaaaataaaa gaagactttc tccaataata aaattaaatg gagaagggaa
     9421 aggtatatta tcatcttcaa ctactagaag agaaggtatt gtaacaaatt tagatgttgg
     9481 ggttgaaata ttagacaact ttaatataaa aaatcaaaat atggttggaa gaaaatatga
     9541 acttattaac agagatgata ataaagaatt tttgatggat gaatatcaaa agatagtatc
     9601 tatttcaagc ataaggtcta caatactgaa tggttttgta agtatagttt tcttgtcatg
     9661 gatagttgct atgattgcaa ttttatttag aaagcatata tcaaaaaatt ataaagaaac
     9721 aacatttttt atattaaagg aacttttaaa aataggaata gtaatgccac tgtcatttat
     9781 gataacacca ataatgaatt tcaaaacacc attagcaata tctttaggaa taattataat
     9841 aacacttact atatatctta taagtaaagt tttaataaag aacgatttaa aaaacatgct
     9901 gttttttact ggattaacca tagtaattat ggttattgat gctgggtttg gctcatattt
     9961 aatgaaaagc aatgttatga gttatgattg tataattgga gctagatatt atggagtagg
    10021 aaatgagtat caaggtgtgg caatcggtag tgcaatattt acatttgcaa ttttacttac
    10081 atataaaaat ataccaaagt ggtctataat agttttttct ttggttatct taattacaag
    10141 tgcatctcca attatgggag caaatgttgg aagtgctatt tctgaatgtg ttgcattttt
    10201 actatttata ctattgatat ataatgtaaa gatagatttc aaaaaaatcg tattattagg
    10261 tatagcagta gtatttgtat taggagtttt tgtagcaata gatatgattt tgggaagtaa
    10321 ttcacattta ggcatgtttg taaaagaaat ttactttaat ggaccaggag aaataataca
    10381 aacatttagt agaaagatag agatgaattt aaagctagct caaaccagtg catgggtaaa
    10441 tatactgtta actggaatag gtgtaatttt agttcttatg attaatcaga ttagatactt
    10501 taagcaactg atggatgagt atccaattgt atttaaagga tttatagcat caatagcagg
    10561 atgtttagta actcttttag tgaatgactc tgggattgtg tcatcagcta cagcctttat
    10621 ttatgtaatt gtacctatga taacattaag tataaatctc acagcattaa aagaataagt
    10681 actgtatgaa taatatgt
//
LOCUS       DAELLV010000125        10594 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.125, whole genome shotgun sequence.
ACCESSION   DAELLV010000125 DAELLV010000000
VERSION     DAELLV010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10594
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.125"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            145..3042
                     /locus_tag="KPD58_002396"
     CDS             145..3042
                     /locus_tag="KPD58_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG0266158.1"
                     /translation="MKAFKKHFLILSLIFLLIISCFNNILCFADESENSKKIRVGYCD
                     DYGIISSSKEHSYTGYGYDYLREISKYTRWEYEFVKGSWEECLDRLEKGEIDLLGPLQ
                     KNDERNKLFNFPKLSSGYEYSALYTKDSNNNMFYEDISSFKGMRVAVLRGNFHNTAFE
                     KYREENNFSVEYIYCNSIDELIESVNEKKADAFVCGSIINAKGMKIVSKFSVEPFYFA
                     TAKDKPNLVKELDYALKELKINDMYYELELYKKYFKREVNNSIAFTRQEMNFIKANPK
                     LTMVYDSEWSPVEYYDKESNSFKGISSDLMSIISKKCGIKFEYIKTKNYNESLDYIKS
                     GKATMLCGSINENDKAKRFNMKLTNPYINIPMIMVGKLDTNLNNDLNIALTSSYKSID
                     SYIEKSFENAKTTSYKSVESCLNAINEGKANLMILSSYQFDELLREGKSENLSVISVL
                     DTSYGMRIGVSNKTDPILVSILNKSIDKITEEELSDCIYSNTIDKPYKVPFGVIFKEY
                     SIQIISFVCILFLIAIKYIIYNKKKKEDYLRKIAYTDPLTGADSIDKFKINSNKLFAK
                     NNPEEYALFYIDVDKFKYINDMFGYDMGNDTLIHISNTIASELKEDEIFARVSADHFV
                     LLIKYKTDDDIKTRLNNIYNKVQILSNPKINYYKLILDCGIYKISKSDNDINTIMDRA
                     NTARKTIKGGHKNSFAFYDKEMHKKILKEKEIENSMVDALNNGEFIVYFQPKYSLSDY
                     QIIGAEALVRWDNPQKGLIPPIEFIPVFERNGFIVNIDFYVFEEVCKKIREWMDEGQK
                     VVPISVNLSRMHFVNSNFIEKFKLIVDKYKIPTRLIELELTETAVLDNIEGLLDTMNN
                     LKEKGFVISMDDFGTGYSSLNLLKELPVDILKLDRAFFTEKDESNNEKIVISNVIKMA
                     KELKMKVISEGVETISQVEFLKQIGCDMVQGYLFSKPMPVKEFEKIAFKKE"
     gene            3363..3575
                     /locus_tag="KPD58_002397"
     CDS             3363..3575
                     /locus_tag="KPD58_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG0266159.1"
                     /translation="MMPLILSNTGEEVVIKKISGSDKTRTFLNSIGFVVGTNVKIVSK
                     IDGNLIIDVKDTRVALDKKMASRIII"
     gene            3588..3809
                     /locus_tag="KPD58_002398"
     CDS             3588..3809
                     /locus_tag="KPD58_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG0266160.1"
                     /translation="MNRLKDIKCGETVKVKKLEGEGATRRRIMDMGITRGVNIFIRKV
                     APLGDPIEVTVRDYELSIRKSDAEKIIVE"
     gene            3909..6065
                     /gene="feoB"
                     /locus_tag="KPD58_002399"
     CDS             3909..6065
                     /gene="feoB"
                     /locus_tag="KPD58_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBG0266161.1"
                     /translation="MSIKIGLIGNPNCGKTTMFNGLTGSSQYVGNWPGVTVEKKGGKL
                     KGNKDVEIVDLPGIYSLSPYTLEEVVTRNFMLDDKPDAVINIVDASNIERNLYLTTQV
                     LELGIPTVIALNMMDIVNKNGDKINIKELSEVIGCPVVEVTAVKGQGIMEAAEKAVEL
                     ASSNNKLNFKLPFVDESKDAIEKIEKIIEEKTPYIDVETRWLAIKLFERDENVIQKLD
                     ISKTILNSIEEITRNCEDELDDDSESIITANRYEFISSIISSIIKKNRKGKETVSDKI
                     DKIVTNRILALPIFALIMWGVYYIAVSSLGTIATDWTNDVLFGEIIQGNVSNFLASLN
                     VAEWLQGLVVDGLIGGVGAVLGFVPQIMLLFLLLSILEDCGYMSRVAFIMDRIFRKFG
                     LSGKSFIPMLISSGCGVPGVMSTRTIENDRDRKMTIMLTTFIPCGAKIPIIALFAGAL
                     FGGASWVAPSMYFLGIAMIIICGIILKKTSLFAGEPSPFVMELPQYHIPSAKGVLIHM
                     WDRGKAFIIKAGTIIFVACGVIWFLQSFNWSLQMVDAGDSILASLGNIVAPIFAPLGF
                     GNWQSSVATVTGLVAKENVVGTFGVLFGISDATEQDPTLLASVASMFTVASAFAFMAF
                     NMLCAPCFAAIGAIKREMGSWKWTWITLGFQTLTAYIIALLINQVGSLVLGTGGSIAG
                     AIISIFIAVAVVFVVLTYSNKNMKKEKMGKLSYMKN"
     gene            6091..6243
                     /locus_tag="KPD58_002400"
     CDS             6091..6243
                     /locus_tag="KPD58_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HBG0266162.1"
                     /translation="MATFIIAAIVVVLMALAVMYMVKNSKKNGSSCGCGCSGCSSSKS
                     CHSAKR"
     gene            6423..7133
                     /locus_tag="KPD58_002401"
     CDS             6423..7133
                     /locus_tag="KPD58_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HBG0266163.1"
                     /translation="MFKFDVTNELMCIARGSGKFFAKKGAMVAFKGNFNFEKLLLGPS
                     NGGGLGRALLGHVQRSLTGEQMPIMVVEGEGEIYLAQNAYHVDVISIDPGDSIYVESE
                     NLLAFSEQLNYSVKLIGSGVISQKGLFTTHLVNKTGQNQDVAIITDGNPLILEGPCCV
                     DPDALVAWTGREPYPKVSKLSWKTFIGQTSGESYHMEFVEPGQIVIIQPSERLSGLKT
                     DSTPSDRMSSGGLRLGID"
     gene            7218..7988
                     /locus_tag="KPD58_002402"
     CDS             7218..7988
                     /locus_tag="KPD58_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0266164.1"
                     /translation="MFEKIHKRLKGYYIFLIILILWKIVCVLGIWSSYILPPPEIVLD
                     TFIKMIYDGSIFLNVYASVRRILIGFLISSLMAIPLGVLFGANKKMYEYFKPLFEFMR
                     STPPLALVPMLILWFGIGEESKIIIIILASFFPIFLNTLKGIKNCDNKLIEVGKSFNL
                     SKKRIFYKIIIPNSMLDIVVGLSLGLGYSFRAIIGAELIAASSGLGYLISDGKDMSRT
                     DVVLVGIFVIGFLGILSDYLFSKIITKFSKGKQVDLNG"
     gene            7981..8712
                     /locus_tag="KPD58_002403"
     CDS             7981..8712
                     /locus_tag="KPD58_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0266165.1"
                     /translation="MVRSGFLLENIYKKYLVDNKEHLVLDNISLNISSEEITVILGES
                     GCGKTTLLRILAGLENATSGNVYFFNNDKKCTPKVGMVFQESRLMPWLNVSENILLHT
                     EKDNRNKVDLDKYLKMMKLEKFKNSYPNELSGGMAHRVSIARALSFNPDILLMDEPFA
                     ALDYFTRRKMQKEVVNIHKNTKKGVVFVTHNIEEAMEIAKKIIVFSKNKRIKQFSVED
                     EYNRDLTKNYYINLKKEILRELGEF"
     gene            8746..9705
                     /locus_tag="KPD58_002404"
     CDS             8746..9705
                     /locus_tag="KPD58_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG0266166.1"
                     /translation="MTSLIMVLVGTVGCSTTNDKKDEPAKKEASLPKEINLTYVKSPL
                     NVPSILEKKDDLFGKEFKKDNIKVNFHELTTGPEQTQALAAGELDFLHALGGTSAIIA
                     ASNGVGLKITNIYSRSPKGYMLITNNDNIKSIKDLKGKKIVGPKGTILHQLLIAALAK
                     EGYTIDDVEYVNMDIPSAASALESKSADVAMLAGPVALKTINSGAKMIVNGEGLVSGI
                     IVTAVSDDFAEKYPELVKRFMKVHEETLKYMNENKDEVMDVVSKEVGLSLDETKEMYS
                     WYDFSSKITDKDIKELEDTQEFLMSNGMQQKKINIKDMLYNQN"
     gene            complement(9803..10399)
                     /locus_tag="KPD58_002405"
     CDS             complement(9803..10399)
                     /locus_tag="KPD58_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HBG0266167.1"
                     /translation="MSCCNSQFCCKNKIESSYIKWLLEVLGPVFLGSKPAEIINISFT
                     DVNREEKINDIYKYLYKCKKIDFIVIDKEKKGLKILFVNKKALSEKLKCKKTVNFLKF
                     LGYKQNTNVNAYLEHLVDKLKSDVFPDEIGIFLGYPLKDVIGFMGYSNYEVSMIKYWK
                     VYGDTKQSEDTYSKFLLHRKKMRKLLDYISVDKIVSCF"
BASE COUNT         4095 a         1103 c         1896 g         3500 t
ORIGIN      
        1 tagataataa taaattcatt atagttgaat aagaatatta tatgagaata ttgcaatttg
       61 atatatttac cattacaata aaagtagtta caactagaaa accaaataag ttaaagtaag
      121 ttggcaaatt ggaggttttt aaatatgaaa gcatttaaaa agcacttctt gatactttct
      181 ttgatatttt tacttattat atcatgtttt aacaatatac tatgctttgc tgatgagtct
      241 gaaaattcaa aaaaaataag agttggatat tgtgatgact acggaataat atcctcatca
      301 aaagagcatt cttatacagg atatggttat gattatctaa gagaaatctc aaaatataca
      361 agatgggaat atgaatttgt aaaagggtct tgggaagaat gtttagacag actagaaaag
      421 ggtgaaatag acttactcgg acctttacag aagaatgatg aaagaaataa actatttaat
      481 tttcctaaac taagttcagg atatgaatat agtgcactat atacaaagga tagtaacaat
      541 aatatgtttt atgaagatat aagttccttt aagggaatga gagtagctgt acttagagga
      601 aactttcata atactgcatt tgagaagtat agagaagaaa ataatttttc agttgaatat
      661 atatactgta attctattga tgaattaatt gagtccgtca atgaaaaaaa ggcagatgca
      721 tttgtttgtg gaagtattat aaatgctaaa ggaatgaaaa tagttagcaa attttcagta
      781 gaaccttttt attttgctac tgcaaaagat aaacctaatt tagtaaaaga attagattat
      841 gctttaaaag aattaaaaat aaatgatatg tattatgagt tggaattata taaaaaatat
      901 ttcaaaagag aggtcaataa ttccatagca tttactcggc aagaaatgaa ttttattaaa
      961 gcaaacccaa aacttactat ggtatatgat tcagagtggt ctcctgtaga gtattatgat
     1021 aaagaaagca attcatttaa aggtataagt tccgatttaa tgtcaattat atcaaaaaaa
     1081 tgtggtataa aatttgaata tattaaaaca aaaaattata atgagagtct tgattacata
     1141 aaaagtggta aggcaactat gctatgtggc tcaatcaatg aaaatgataa ggcaaaacga
     1201 tttaatatga aattgacaaa tccatacata aatattccaa tgattatggt aggaaaattg
     1261 gataccaatc tcaataatga tttaaatatt gctttaacta gtagttataa gtctattgat
     1321 tcttatatag aaaagagttt tgaaaatgct aagacaacat cttataagag tgtagaaagt
     1381 tgtctaaacg ctataaatga aggtaaagca aacttaatga tacttagctc ttatcagttt
     1441 gatgaacttt taagagaagg aaagagtgag aatttatctg ttatatctgt tttagataca
     1501 agctatggga tgcgtatagg ggtatcaaat aaaactgacc ctatattagt gtctatttta
     1561 aataaatcaa tagacaaaat tactgaagaa gaattaagtg attgtatata ttctaatact
     1621 attgacaaac cttataaagt accatttggt gtgattttta aagaatattc aatacaaatt
     1681 atttcttttg tatgtatttt attcttaatt gctattaaat atataattta taataaaaag
     1741 aagaaagagg attatctaag gaagatagca tatacagacc cattgacagg agcagatagt
     1801 atagataaat ttaaaataaa ttctaataaa ctgtttgcta aaaataatcc agaagaatat
     1861 gcgttgtttt atatagatgt tgacaaattt aaatatataa atgatatgtt tggatatgat
     1921 atgggaaatg atactttgat acatatatca aatactattg caagtgaatt aaaggaagat
     1981 gaaatttttg caagagtttc tgctgaccat tttgttcttc ttattaagta taagacagat
     2041 gatgatatta aaacaaggtt aaacaatatt tacaataagg ttcaaatact tagtaatcct
     2101 aaaataaact attataaatt gattttagat tgtggtatat ataaaataag taaaagtgat
     2161 aatgacataa atactattat ggatagagct aatactgcaa gaaaaactat aaaaggtggt
     2221 cataaaaatt catttgcttt ttacgataag gaaatgcaca aaaaaatatt aaaagagaaa
     2281 gaaattgaaa attcaatggt agatgctctt aataatggag aatttattgt ttattttcaa
     2341 cctaaatata gtctttcaga ttatcaaatt ataggggcag aggctcttgt tcgttgggat
     2401 aatccacaaa aaggtctaat accaccaata gaatttattc ctgtatttga gaggaatggt
     2461 tttattgtaa atattgattt ctatgtattt gaggaagtgt gtaaaaagat tagagaatgg
     2521 atggatgaag gacaaaaagt agtacctata tctgtaaact tgtctcgaat gcattttgta
     2581 aatagtaatt ttatagagaa gtttaaatta attgtagata aatacaaaat tccgacaaga
     2641 cttatagaat tagagctaac agaaacagca gtattagata atatagaagg attacttgat
     2701 acaatgaaca atttgaaaga aaaggggttt gtaatctcta tggacgattt tggtacagga
     2761 tattcctctc ttaatctttt aaaagaattg cctgttgata tattaaaact agatagagca
     2821 ttttttacag aaaaagatga gagtaataat gaaaaaattg ttatatctaa tgtcattaaa
     2881 atggcaaagg aactgaaaat gaaagtaata tcagaaggtg tagagactat ttcgcaagta
     2941 gaatttctaa agcagattgg gtgtgatatg gtacaaggat atttgttttc taagccaatg
     3001 cctgtaaaag agtttgaaaa aatcgctttt aaaaaagaat aaagcaatgt gtaaataatg
     3061 acatatataa taaaggtgct ttaatattag ttgagtatac tattttagag catctttatt
     3121 tttgtattaa tttttacgat tttattaata aaataaatca aatgagaaaa aaaatcaaaa
     3181 gaatacaata atcacttgca aaaaatatac tattgtgata gacttaaatc aaaattagga
     3241 attgtaaaaa gaaggaaaaa ataggatatt attttaatag attaagattt aaggtattgt
     3301 aatttatata taagataacg attgcttgta aatttaaatt actttaagag gagggatatt
     3361 atatgatgcc attgatatta tcaaatacag gtgaagaagt agttataaag aaaatttcgg
     3421 gaagtgataa gacgaggact tttttaaata gtataggttt tgtagtgggt acaaatgtta
     3481 aaatagtttc taagatagat ggtaacttaa taatagacgt aaaagatact agagttgcat
     3541 tagataaaaa aatggcaagt agaataataa tatagggagg taatgacatg aacagactga
     3601 aggatattaa gtgtggagag actgtaaaag tcaaaaagtt agaaggagaa ggagctacac
     3661 gaagacgtat aatggatatg gggattacaa gaggtgttaa tatatttata agaaaagtag
     3721 ctcctcttgg tgacccaatt gaggtaacag tcagagatta tgaactatca attcgtaagt
     3781 cagatgcaga aaaaattatt gtggaataat aaagattaat tagagtaggg ttaatgtaat
     3841 catataatga gaatgatttt ctgtatcaca aacagtgatg tatttaaata ataatggagg
     3901 gaatggaaat gtcaataaaa ataggtctca taggaaatcc taactgtggt aagacaacaa
     3961 tgttcaatgg gcttacaggc tcatcacaat atgttggtaa ctggccagga gtaacagttg
     4021 aaaaaaaagg tggaaaatta aaaggaaata aagatgtgga aatagtagat ttaccaggta
     4081 tttattcttt atctccatat acattagaag aagtagttac acgtaatttt atgctagatg
     4141 ataagccgga tgcagtaata aatattgtag atgcatcaaa tatagagaga aatttatatc
     4201 ttacaactca agttttagag ctaggtatac cgaccgttat agctcttaat atgatggata
     4261 ttgtaaataa gaatggtgat aagataaata ttaaagaact atctgaagtt ataggttgcc
     4321 cagtggtaga agtaacagca gttaaaggtc aaggaattat ggaagctgct gaaaaggcag
     4381 ttgaacttgc aagtagtaac aataaattaa atttcaaatt accctttgtt gatgaaagta
     4441 aggatgcaat agaaaaaatt gaaaaaataa tagaagaaaa gactccatat atagatgtag
     4501 aaacacgctg gttagctata aagctatttg agagagatga aaatgttata caaaaacttg
     4561 atatttctaa aacaatattg aatagtattg aagaaataac tagaaattgt gaagatgaat
     4621 tggatgatga tagtgaaagt ataataacag ccaatcgtta tgaatttatt agttcaataa
     4681 tttcaagtat aataaagaag aatcgaaaag gtaaggaaac tgtatcagat aagatagata
     4741 agattgtaac aaatcgtata ctagcattac ctatttttgc acttataatg tggggagttt
     4801 actatatagc agtaagttca ttgggtacta tagcaactga ttggacaaat gatgtactgt
     4861 ttggagaaat tattcaaggc aatgtaagta attttctagc ttctttaaat gtagcagagt
     4921 ggttacaagg acttgtagtt gatggtctaa ttggtggagt tggagcagta ctaggatttg
     4981 taccacaaat catgctttta ttcttattat tatcaatatt agaagattgt gggtacatgt
     5041 cacgtgtagc ttttataatg gatagaattt tccgtaagtt tggtctttca ggaaaatcat
     5101 ttataccaat gcttataagt tcaggttgtg gagttccagg agttatgtca actcgtacta
     5161 tagagaatga tagagataga aaaatgacta ttatgttaac tacattcatt ccatgtggag
     5221 ctaagattcc aataatagca ttatttgcag gggcattatt tggaggtgct tcatgggtag
     5281 ctccatcaat gtatttctta ggtattgcaa tgataatcat ttgtggaatt atattaaaga
     5341 aaactagttt atttgcagga gaaccatctc catttgttat ggaattacct caatatcata
     5401 ttccaagtgc aaaaggtgtt ttaatacata tgtgggatag aggaaaagca tttataatca
     5461 aagcaggaac aattatattt gttgcttgtg gagtaatctg gttcttacaa tcatttaatt
     5521 ggtcattaca aatggtagat gctggagata gtatattagc tagcttagga aatatagttg
     5581 caccaatatt tgctcctctt ggatttggaa attggcaatc atcagtagca acagttacag
     5641 gtctagtagc taaggaaaat gtagttggaa catttggagt tttatttggt atatcagatg
     5701 caacagagca agacccaaca cttcttgctt cagttgcaag tatgtttaca gtagctagtg
     5761 cgtttgcatt tatggcattt aatatgcttt gtgcaccttg ttttgcagct attggagcca
     5821 taaaaagaga aatgggttca tggaaatgga catggattac attaggattc caaactttaa
     5881 cagcgtatat aattgctctt cttataaatc aagttggaag tttagtttta ggtactggag
     5941 gaagtattgc tggggcaata atatctatat ttatagcagt agcagtagtc tttgtagttt
     6001 taacttattc aaataaaaat atgaaaaaag aaaaaatggg taaattaagt tatatgaaaa
     6061 attaattcat ataaataagg agtgatgaat atggctacat ttattatagc agctattgta
     6121 gtagtattaa tggcactagc tgtaatgtat atggttaaaa attcaaaaaa gaatggtagc
     6181 agttgtggat gtggttgttc tggatgctca agtagtaaga gttgtcatag cgctaagaga
     6241 taaataggat aagagcctga taaaaagtta aattgcttta ttatcaggtt cttatttttt
     6301 gtataaataa caaatttcat atttgaagta ggttagacta tgctgtaatt ttactgtata
     6361 attatatata aatatatgat taggagggat tattgctaaa ttaagcaata taagtgaata
     6421 ttatgtttaa atttgatgtt acaaacgagc ttatgtgtat agcaagggga agtggtaaat
     6481 tttttgcaaa aaaaggtgct atggtagcct ttaaaggaaa ttttaatttt gaaaaattgc
     6541 tgttaggtcc aagtaatggc ggtggattgg gaagagcatt attaggtcat gttcagaggt
     6601 cattgacagg agaacaaatg ccaataatgg tggttgaagg tgaaggtgaa atttacctag
     6661 ctcaaaatgc ttaccatgta gatgtaatat caattgaccc aggtgattcc atttatgttg
     6721 agagtgaaaa cttattggca ttttctgaac aattaaatta cagtgtaaaa ttgattggtt
     6781 caggggtaat atctcaaaaa ggtcttttta ctacacattt agtaaataaa acaggtcaaa
     6841 atcaagatgt tgctataata acagatggta atcctctaat tcttgaagga ccttgttgtg
     6901 ttgacccaga tgcattagta gcttggactg gaagagaacc atatcctaaa gtatcaaaac
     6961 tatcatggaa gacttttatt ggtcagactt caggagaatc ttatcatatg gaatttgttg
     7021 aacctggaca aatagtcatt atacaaccat ctgaaagatt gtctggctta aaaacagatt
     7081 ctacaccttc tgatagaatg agttcaggtg gattaagact tggcatagat taaatcttat
     7141 gatatagctt aattattatt taagatatta aaaaataaaa agggtagcag ggatagatat
     7201 gttaaaagga gttttatatg tttgagaaaa ttcataaaag gcttaaagga tattatattt
     7261 ttttaataat tttaatttta tggaagatag tatgtgtttt gggtatatgg agtagctata
     7321 ttctgcctcc tcctgaaata gtcttagata cttttataaa aatgatatat gacggttcta
     7381 ttttcttaaa tgtttatgcc agtgtaagaa ggatattaat aggtttttta ataagttcat
     7441 tgatggctat accacttgga gttttatttg gagcaaataa aaaaatgtat gaatatttca
     7501 aaccactatt tgaatttatg cgaagtacac ctccactagc tttggtgcca atgcttattt
     7561 tatggtttgg tataggtgaa gaatctaaaa ttataattat tattttggca tccttttttc
     7621 caatattttt aaatacattg aaaggtataa aaaattgtga caataaatta atagaagtag
     7681 gtaaatcttt taatttatct aaaaagagaa ttttttataa aataataatc cctaattcta
     7741 tgctggatat tgttgttgga cttagtttag ggcttgggta tagttttagg gctataattg
     7801 gagcagagct tattgcagct tcttcaggac taggatattt gatttctgat ggaaaagata
     7861 tgtctagaac tgatgttgta ttggtaggaa tttttgttat tggattttta ggtatacttt
     7921 cagattatct attttcaaag ataataacaa aatttagcaa agggaagcag gttgatttaa
     7981 atggttagaa gtggattttt gctagagaat atatataaga aatatttagt tgataataaa
     8041 gagcatttag ttcttgataa tatatcttta aatatatcta gtgaagagat aactgttata
     8101 ttaggtgaaa gtggatgtgg gaaaacaacg ctacttagaa ttttagcagg gcttgaaaat
     8161 gctactagtg gaaacgtata tttttttaat aatgataaaa agtgtactcc aaaagtagga
     8221 atggtatttc aagaaagtag acttatgcca tggctgaatg taagtgaaaa tatactttta
     8281 catactgaaa aagataatag aaataaagtt gatttagaca agtatttaaa aatgatgaag
     8341 cttgaaaagt ttaaaaactc atatccaaat gaattatcag gaggaatggc tcacagagta
     8401 agtatagcaa gagcactttc cttcaatccc gatattttgc tgatggacga accatttgca
     8461 gcactagatt acttcacaag aagaaaaatg caaaaagaag ttgtaaatat ccataaaaat
     8521 actaaaaaag gtgtagtatt tgtaactcac aatattgaag aagctatgga aatagcaaag
     8581 aaaataattg tttttagtaa aaataaaagg attaaacaat ttagtgtcga agatgaatat
     8641 aatagagatt taactaaaaa ctattatata aacttgaaaa aagaaatatt aagggaattg
     8701 ggggagtttt aattgattaa aagaaacaaa aagattttag cagtaatgac atctttaata
     8761 atggtgttgg ttgggactgt aggatgttca acaacaaatg ataagaaaga tgaacctgca
     8821 aaaaaagagg ctagtttacc taaggaaata aatttaactt atgtaaaatc accacttaat
     8881 gttcctagta tattagaaaa aaaggatgat ttatttggaa aggaatttaa aaaggacaat
     8941 ataaaagtca atttccatga attgacaaca ggaccagagc aaactcaagc attagcagca
     9001 ggtgagctag actttttaca tgcattagga ggaacgtctg caataatagc tgcatcaaat
     9061 ggtgttggtc ttaaaattac gaatatttat agccgttcac caaaaggata tatgcttata
     9121 actaataatg ataatattaa atctataaaa gacttaaaag gtaaaaaaat tgtaggtcca
     9181 aaagggacta tacttcatca actgttgatt gctgcacttg ctaaagaagg ctatactata
     9241 gatgatgttg aatatgtaaa tatggatatt cctagtgctg cttctgcatt agaaagtaaa
     9301 agtgctgatg tagcaatgct tgcaggtcca gtggcattga aaacgataaa ttcaggtgcc
     9361 aaaatgattg taaatggaga aggattagta agtggaataa tagttactgc tgtaagtgat
     9421 gactttgctg aaaaatatcc tgaattagta aaaagattta tgaaggttca tgaagaaact
     9481 ttaaaatata tgaatgaaaa caaggatgaa gtgatggatg ttgtatctaa agaagttggt
     9541 ttatctttag atgaaacaaa agaaatgtat tcttggtatg actttagttc taaaataacg
     9601 gacaaggata taaaagaatt agaagataca caggaatttt tgatgtctaa tggaatgcaa
     9661 caaaagaaaa taaatattaa agatatgttg tacaatcaaa attagaatac tttaaaatat
     9721 taaagtggaa aatacaccct tatttattat ggaaactgta tggtaaaaga gggtgtattt
     9781 ttattattat acaaaaacac aattaaaaac agcttactat tttatctaca cttatgtagt
     9841 ctagtaattt tctcattttc ttacggtgaa gtaaaaactt cgaatatgtg tcctcggatt
     9901 gttttgtgtc accataaact ttccaatatt taatcataga aacttcataa ttactatatc
     9961 ccataaatcc aatcacatct ttaagaggat atcctaaaaa aatacctatt tcatcaggaa
    10021 aaacatcact cttaagttta tctactaaat gctctaaata agcattaaca ttcgtatttt
    10081 gtttgtatcc taaaaacttt aaaaagttta ctgttttttt acattttaat ttttctgata
    10141 atgctttttt atttacaaat aagattttta atcccttttt ttctttatca ataactataa
    10201 aatctatttt cttacactta tatagatatt tataaatatc attaattttt tcctctctat
    10261 ttacatcagt aaacgaaata tttattattt cagcaggttt agagcctaaa aagactggac
    10321 ctaaaacttc taaaagccat tttatatatg atgattcaat tttattttta caacaaaatt
    10381 gtgaattaca acaagacata aaagataacc tccttcaaat gaaaattaat atcaagtaat
    10441 tagattatac tatgaatgat aatcaatatc aatgataaaa ttataatttt tttatttaat
    10501 tatttattca ttatgatttc caaaaaaata aattataact tgtttaattt tagtaaataa
    10561 aatataaaaa tatagaaata ctaaattaag aaag
//
LOCUS       DAELLV010000126        10495 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.126, whole genome shotgun sequence.
ACCESSION   DAELLV010000126 DAELLV010000000
VERSION     DAELLV010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10495
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.126"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            115..957
                     /locus_tag="KPD58_002406"
     CDS             115..957
                     /locus_tag="KPD58_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HBG0266168.1"
                     /translation="MKEKAGIRNAIIAAFIITILAELIGPISFKVMGINVTLLSILWA
                     IFIGMAVSPHLLGRVIPALKKFIGDNEINVSPYLLSLTLYPLGIMFGINAGPKVGILL
                     QAGPALLLQEFGHMGTMLIALPVGVALGLGRSALGGTFSLCRDTALGIIGDKYGLNSP
                     EGIGTLGTYISGSIFGTLLFSFLAPVGVLLGFHPYALAMASGMGSGSMMGAATASLMN
                     TVPQMSEQILAYSATSGLITAVTGIYIELFLSLPVANYYYSKVAPVIERFRGRKTRRT
                     SNKL"
     gene            972..1436
                     /locus_tag="KPD58_002407"
     CDS             972..1436
                     /locus_tag="KPD58_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266169.1"
                     /translation="MEKTFKTIMEQIKIVLFIYIIICFGQMIALKTQAQDLLIGSVSG
                     FLLVIIAIIIKNFVKKPNLPGFAWATLVAFVLSLPISPVGDIIATNVGKINFMSTVTP
                     LLAFAGISVGNQLDILKTLSWKLVVISFIVMTSTYFGSAFISQTVLRLNGML"
     gene            1423..2595
                     /locus_tag="KPD58_002408"
     CDS             1423..2595
                     /locus_tag="KPD58_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG0266170.1"
                     /translation="MECFKEVVNMNELKAKIKEEIKLLSEEKKTSFEKVSDYLFSNPE
                     LAFEEYKSQKALCDLLEENEFNVTKGVGGLETSFEAVYSNGTNGKTVAFLAEYDALPG
                     MGHACGHNIIGTSSVGAGIILKEIMKKHNIEGTVKVFGTPAEERVGGKITMIKEGVFN
                     NVDAALILHPSDASMPDDISFAQVNLKFDFTGKASHAAAFPWEGKSALSGVIALFNSV
                     NSMRLHLKDYARVHGIITDGGSIHNIIPEKSTAIFNVRALSIEYLNEICEMLKNCAKG
                     AAISTGTNVEIVQLDEIYKEIKNDSELVNIVRQNFEVLGEDYVERDLSQGIGSTDTGN
                     LTHEIPAIQAYIKLKENTATHTDEFAVAAGGEEGRVALIKAIKVLAMCGVDILYSK"
     gene            2639..3574
                     /locus_tag="KPD58_002409"
     CDS             2639..3574
                     /locus_tag="KPD58_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1177 domain-containing protein"
                     /protein_id="HBG0266171.1"
                     /translation="MILKQVIEVYDILDKANANGEEVKTYLQGYGEVDVTVKELSSSK
                     GSTDLVKLTIPGKNGKLKGGDAPTLGILGRLGGIGARPEVIGFVSDGDGALVAIAVAA
                     KLLDMQRKGDILDGDVVISTHICPDAPTKEHYPTPFMDSPIDMMTMNENEVDSSCDAI
                     LSIDTTKGNRVINTRGFAISPTVKEGYILKTSDNLLDIMQTVTGKSPFVFPLSIQDIT
                     PYGNNLYHLNSILQPAVATDAPIVGVAITTEMPVAGCATGATNFSDLDSAGRFAIEVA
                     KLFGRNKCDFYDKEEWEMLIKRYGKLNRFQTFGIQ"
     gene            complement(3945..4670)
                     /locus_tag="KPD58_002410"
     CDS             complement(3945..4670)
                     /locus_tag="KPD58_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG0266172.1"
                     /translation="MLESKIRTPEEIKRYMLDLKKWLAQERSKPVEEMSDFFSKRIDI
                     YDNVHLANWTEEYAHIADYFDTPLSSLLDIGCGTGLELASIYRKFPNVQITGIDLSKE
                     MMSKLKEKYSDKNIDLILADYFEYPFEQEKYDAALSFETLHHFKYQKKQKIYKKLFQT
                     IKPGGYYVECDYIACCLEEETICLEQYEFKCKENHVPDDVFVHIDIPLTIEHQIHLMK
                     NAGFRAVYVLHENNGTMIIKAEK"
     gene            complement(4688..5254)
                     /locus_tag="KPD58_002411"
     CDS             complement(4688..5254)
                     /locus_tag="KPD58_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0266173.1"
                     /translation="MPPKPIITKQDIINAAIQLVRENGISSVNARNLAKTLNCSTKPL
                     FRIYTNMDEIKEDIKIELDNYYNSFMESKINDENRLLSQGLAYIEFAQNEKMIFNTLF
                     MNMTMKGSSLQDIIHAKWNRITIENAKKVTGLSIEKSEMLFINFWLYSHGVATQIISN
                     GIDIPLDMVQQLLENAFERFSLDITKTE"
     gene            complement(6165..6563)
                     /locus_tag="KPD58_002412"
     CDS             complement(6165..6563)
                     /locus_tag="KPD58_002412"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBG0266174.1"
                     /translation="MKWKELYNKARGKLNPRTISPFIDAGGVAAAILTDSGNVYTGVC
                     IDTACTLGMCAERNAIANMITNGESKITKLVCVMSNGSVGSPCGACREYLMQLDKDSA
                     CIEILVNVETEKTVKLSELLPDWWGSDRFI"
     gene            complement(6862..9555)
                     /locus_tag="KPD58_002413"
     CDS             complement(6862..9555)
                     /locus_tag="KPD58_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HBG0266175.1"
                     /translation="MNNDNDKNGNKIRRKKVSSSGNTNKPINRTSANKTRSTKNKKKS
                     KKSDKFKKLRVFGIVFLVLLVVGTAGTAGLVFASLRDVTPVTEAVLDKQTNQTTTIKY
                     ANGKTLSTAPSVNKKTPVPLDKISPYLQHAVIAIEDERFYEHKGVDIKGLFRSVLKTL
                     TGTKQGGSTIPMQVSKMLLTTEQQTIPRKIKDIYYAHEMSKTVSKDKILETYLNNFFV
                     GRGLAGAEAGARGYFDKSAADLTLAESALLAGSTKNPSRFSAYKTSKLEGNETKEDLE
                     NKLLFFVNTTDDDLDDPTQVDFDMIEKIKSWELISNDTYRQLKAGTLVVRKAVSNPEA
                     KKRQEIVLKKMLELKYIKQSEYDEAIKAKIEIKLPQSSDKVSSSVEDLIESEVINALM
                     EQGHTNDEAQNLFYNGGLIVNTTIDPKMQDALEEEFDRNSNFPGHMVGPDGVSQPQAA
                     MVILDYKNGEIRALAGGRNISGRKTLNRATNPHQPGSSIKPLAIYTPAIDTLKITQAT
                     ALSDSRGGYKFEENNKWNPRTTTAGHGSMSLRKALAKSSNTIAVKTAEMLGDSYDECV
                     DIMMDYLKNFGITTLKNNHSGSSEASDRKFPSLTLGGMANGITPLQMAAAYGTLANHG
                     IYVEPSIFTTITTFDGQLLVKNAPEEHKVVEPEVAYVVTDMLESVITEGTGGVAALPK
                     GMPVAGKTGTTNSAYDAWFVGYTPYYVGATYIGDDAGRKDDSGNTIKRREVPHGSTST
                     AKLWEKIMEKIHANLTVTEFEVPKNVYFTKINLEDGGKQSSGSKAAFIEGTAPTKVSS
                     QPSSEDTKKPDNNQPEENNNNNNNTGDNGGGSTPPDNGGNNGGGSTTPPDNGGNNGGG
                     SATPPDNGGNNGGGSTTPPDNGGNNGGGSTTPPDGSGTPAT"
     gene            9769..10467
                     /gene="yunB"
                     /locus_tag="KPD58_002414"
     CDS             9769..10467
                     /gene="yunB"
                     /locus_tag="KPD58_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HBG0266176.1"
                     /translation="MNRIVDRKTKNEFRKIIAIFLVILFLSVFIGSFIYIDKTLRPTI
                     TVLAETKALELANRSINKAVAEMVEGKINYEDLMDIQLDNNGKITMIQANTIMMNEIA
                     SAIALEIQDELKKDKTASSYIPIGTALGSPILAKYGPKLEVSIEPIGTVSVNFKTEFE
                     SSGINQTRHRIYLEAQTQVKVVIPLITSTKQIKAQIPICETIIVGDVPESYVNIPEKN
                     LGNVLPNTGKNTNK"
BASE COUNT         3535 a         1628 c         1776 g         3556 t
ORIGIN      
        1 tatataaaaa tgatgaataa atatatatta tacattatca atgataggtt atataataaa
       61 atcactaaca attatattat aagaaaatta taaaaattac aaggaggtat agaaatgaaa
      121 gaaaaagcag ggattagaaa tgccataata gcagccttta taatcacaat attggcagaa
      181 ttaattggac ctatttcatt taaagttatg ggaattaatg taactttact tagtatacta
      241 tgggctatat tcataggaat ggctgtttct cctcatttat tagggagagt tataccagcc
      301 ttaaaaaagt ttatagggga taatgaaata aatgtatctc catatttatt atctttgaca
      361 ctatatccat tagggataat gtttggaatt aatgcagggc ctaaagttgg tatattactt
      421 caagcaggac cagcactttt attacaagaa tttggtcata tgggtacaat gttgatagca
      481 ttacctgttg gagtagcatt aggtcttgga agaagtgcat taggtggtac tttttcatta
      541 tgtcgtgaca cagcactagg aattattgga gataagtatg gattaaactc accagaaggt
      601 ataggaacac taggaactta cataagtggt agtatatttg gaacattatt attttcattt
      661 ttagcaccag tgggtgtact tttaggattt catccatatg ctttagcaat ggcatcagga
      721 atgggtagtg gttcaatgat gggagctgca acagcatcat taatgaatac ggttccacaa
      781 atgtcagagc agattttagc atattcagct actagtggat tgattacagc tgttactgga
      841 atatatatag aattattctt atctttacca gtagccaact actattatag caaagtagct
      901 ccagttattg aaagatttag aggaagaaaa acaagaagaa caagtaataa attgtaaagg
      961 aggatatata tatggaaaaa acttttaaga ctattatgga gcagataaaa atagtattgt
     1021 ttatatatat tattatttgc tttggacaaa tgatagcact taagactcag gctcaagatt
     1081 tgttgattgg aagtgtatct ggatttttgt tggtgataat tgctattata ataaagaatt
     1141 ttgttaaaaa acctaattta ccaggttttg catgggctac attagttgca ttcgttttat
     1201 cgcttccaat atctccagtg ggggatatca tagcaacaaa tgtagggaaa atcaatttta
     1261 tgtctacagt aacaccatta ttagcttttg caggaatctc tgtaggtaac caattagata
     1321 tattaaagac tttgagttgg aagttagtag ttatatcatt tatagttatg acatctactt
     1381 actttggttc tgcttttata tctcaaacag ttttaagatt aaatggaatg ctttaaggag
     1441 gtagtaaata tgaatgaatt aaaagcaaaa attaaagagg aaataaagtt attatctgaa
     1501 gagaaaaaga catcatttga aaaagtttct gactatttat tttcaaatcc agaattagca
     1561 tttgaggagt ataagtcaca aaaagcatta tgtgatttgt tagaagaaaa tgaatttaat
     1621 gtaactaaag gtgtaggagg attagaaaca tcttttgaag ctgtgtattc aaatggaact
     1681 aatgggaaaa cagttgcttt tttagctgaa tatgatgcac ttccaggaat gggacatgca
     1741 tgtgggcaca atatcatagg gacaagttct gttggtgcag gtatcatatt aaaagaaatc
     1801 atgaaaaaac ataatataga agggactgta aaggtttttg gaacacctgc agaagaaaga
     1861 gtaggtggaa aaataactat gattaaagaa ggtgtattta ataatgtaga tgctgcatta
     1921 attttacatc caagtgatgc ttcaatgcct gatgatatat catttgctca agttaattta
     1981 aaatttgatt ttactggtaa ggcatcacat gctgctgctt ttccttggga agggaaaagt
     2041 gccttaagtg gagtaatagc actatttaac agtgtaaata gcatgagatt acatcttaaa
     2101 gattatgcaa gagtacatgg aattataact gatggaggta gcatacataa tataattcct
     2161 gaaaaatcta cagctatatt taatgtaaga gccttaagta tagaatattt aaatgagatt
     2221 tgtgaaatgc ttaaaaattg tgctaaagga gctgcaattt ctactggaac taatgtagaa
     2281 atagttcaat tagatgaaat atacaaagag attaagaatg attctgaatt agtgaatata
     2341 gttagacaaa attttgaagt tttgggtgaa gactatgttg aaagagattt aagtcaaggt
     2401 ataggttcaa ctgatacagg aaatttaact catgagatac ctgcaataca agcatatata
     2461 aaattaaaag aaaatactgc aactcataca gatgaatttg ctgttgcagc tggtggtgaa
     2521 gaaggcagag tagcacttat aaaagcaatt aaggtgttag caatgtgtgg agtagatatt
     2581 ttgtatagta aataatatat aattttaaaa tatattaaga aaggaaatcg gattagacat
     2641 gatattaaaa caagtaatag aggtttatga cattttagat aaagcaaatg ctaatgggga
     2701 agaagtaaaa acttacttac aaggttatgg tgaagtagat gtaactgtaa aagaattgtc
     2761 ttcaagtaaa ggctcaacag atttagtaaa gcttacaatt cctgggaaaa atggaaaatt
     2821 aaaaggtgga gatgctccta ctcttggcat tttaggtaga ctaggaggga ttggagcaag
     2881 accagaagtt ataggttttg tttcagatgg tgatggagca ttggtagcaa ttgcagtggc
     2941 tgctaaactt ttggatatgc aacgaaaagg ggatatatta gatggtgatg ttgtaatttc
     3001 aacccatatt tgcccagatg ctccaactaa agaacactat cctactcctt ttatggattc
     3061 tccaattgat atgatgacta tgaatgaaaa tgaagttgat agtagttgtg atgcaatact
     3121 ttctatagat actactaaag gaaatagagt aatcaatact agaggatttg caatatcgcc
     3181 aactgttaaa gaaggctaca tattaaaaac tagtgataac cttttagata ttatgcagac
     3241 tgtaacaggg aaatctccat ttgtattccc tttgtctatt caagatataa caccatatgg
     3301 aaataatctg tatcacttaa atagtatttt acaaccagca gtagctacag atgcaccaat
     3361 agtaggagta gcaattacaa cagagatgcc tgttgctgga tgtgctacag gtgcaactaa
     3421 ttttagtgat ttagattcag ctgggagatt tgcaattgaa gttgccaagc tatttggtag
     3481 aaataagtgt gatttctatg ataaagaaga gtgggaaatg cttattaaga gatatggcaa
     3541 attaaataga tttcaaacat ttggtataca gtaattagaa aaatattaag tttatataat
     3601 ttgtgatata aatatctata ctatgtaaac aactagaaac aaaagatata gtttaagact
     3661 taaaaaataa aatatttatt tgattacata aataaggagt gttctattta ggtaagcgcc
     3721 aataagtagt ataacattaa agctaaccta attgttgata tacaagattg aaatgaaaac
     3781 agtgcgtatt ataagaaaag gttacgcact gtttttttcg tcctgttata tagcagaaca
     3841 ctagttttta agggtatgag tataaaactt ttttaaattt catatctacg aattttgtag
     3901 attgtccata agcgatatgc aataattagg aaaatatttt gagtttattt ttcagcttta
     3961 ataatcattg tgccgttgtt ttcatgaagt acatacacag ctctaaagcc tgcatttttc
     4021 attaagtgta tttgatgttc aatagtcaaa ggaatatcaa tatggacaaa aacatcatca
     4081 ggcacatgat tttctttaca tttaaattca tattgttcaa gacaaattgt ttcttcctct
     4141 aaacaacagg caatataatc gcactcaaca taatatcctc caggtttaat tgtttgaaaa
     4201 agctttttat atatcttctg ctttttctga tatttgaaat gatgaagcgt ttcaaatgag
     4261 agggcagcat catatttctc ttgctcaaaa ggatattcaa aataatctgc caagattaaa
     4321 tcaatatttt tatctgaata tttttcttta agcttactca tcatttcttt tgataaatca
     4381 atgcctgtaa tttgtacatt aggaaatttt cgataaatcg atgcaagttc tagaccagta
     4441 ccacaaccaa tatcaagtaa agaagatagt ggagtatcaa aataatctgc tatatgtgca
     4501 tattcttctg tccaattagc aagatgaaca ttatcataaa tatcaattcg ctttgaaaag
     4561 aaatccgaca tttcttcaac cggtttactt ctttcttgcg ctaaccattt tttgaggtca
     4621 agcatatatc ttttgatttc ttcaggtgtt cttattttac tttccaacat ataaaacagt
     4681 cctttcttta ttcggtcttt gttatatcca atgaaaatcg ctcaaaagca ttttcaagta
     4741 actgttgtac catatctaat ggaatgtcta tgccattgga aataatttgt gtagctactc
     4801 catgagaata aagccaaaag ttaataaata gcatttctga tttttctata gataagcctg
     4861 taactttttt agcattttct atagtaattc tgttccattt tgcatgaata atatcttgta
     4921 aggatgaacc cttcattgtc atattcataa ataaggtatt gaaaatcatt ttttcatttt
     4981 gagcaaattc aatgtatgca agtccttgac ttaacagacg attttcatca tttattttac
     5041 tttccataaa agaattatag taattgtcta gttctatttt aatatcttct tttatctcat
     5101 ccatatttgt gtaaatacga aaaaggggtt ttgtagagca attaagggtt tttgctaagt
     5161 tacgagcatt tacgctactt attccatttt ctcttaccaa ctgaattgca gcgttaataa
     5221 tatcttgttt tgtaataatc ggttttggtg gcatcttttc atcctccttt cggtaactta
     5281 agttgcgcaa ctggtgttgt tattataaca aaggcatata actgtgtcaa tagacttcaa
     5341 agaaaagcta cagacatcga gtgtaatatg atttatttca ataaatacca taatatgtgt
     5401 acttatatct taaaaaggag ataaacagta aaatgtagtt tagttaaatt tgaaacttcg
     5461 tcaaagcttc tttgtacgat aagacaacgt acaaatcatt gtatgggtaa cagagaaaga
     5521 actgcgttga ttgaggatga aatctcacat gatacgatgt aattataaaa aaaggagcgt
     5581 ttcaaagtga tttttttaat catcttgaga caactcctta tattttttat ttaaattcta
     5641 ttttttattg cctgtatagc tattttctcc atcataaggt ggcatgacct tgaacagcgt
     5701 ggttttgtaa agttgggcac cacaaaacaa aaaatcaaca agcccttgtg tggtctgctg
     5761 acgtgtttac tatactatac tttatccaaa accgcaagca ctatgggagg ctataaggcg
     5821 gcggccattc aggggcgatc ccctcgaaac cgggataggg ttcccatgtg ggacataata
     5881 aagagcgccc cggaggtatc aactagcatc tggggcgctc tttattatgc cccgtgggaa
     5941 ccatgcccac gaagcagggc gtacaacgga cagataggtc ctaccctcct gacaaactgg
     6001 aatctccgaa aacaatcata acaatcatgg caagattata tacactcctg taaaatattt
     6061 ttgtgtgtac catgaagtac tgcttgccac tattgagaac ggataaaagg gaagtacgta
     6121 ttataccaat aaggtgcatg aaacaaaata ctcatttgat aaagttaaat aaacctgtcg
     6181 gaaccccacc aatccggcaa aagctcactc aatttaacag ttttttctgt ttcgacatta
     6241 actaaaatct caatacaagc actatcctta tcaagttgca ttaaatattc acgacaagca
     6301 ccacatggtg agccaacact cccattgctc atcacacaaa ctaatttagt aattttactt
     6361 tcgccatttg ttatcatatt ggcgatagca tttctttctg cacacattcc caatgtacag
     6421 gctgtatcaa tacacacacc agtataaaca tttccgctgt cggtaaggat tgctgcggca
     6481 accccaccag catcgataaa aggtgaaatt gtgcgtggat ttaattttcc cctagcctta
     6541 ttataaagtt ccttccattt catcaggttg atcctcctgt tgtatccata taaataatct
     6601 tttatacgcc ttttccgcct aaggcacaca acacattact cgcttttttc attctaatat
     6661 tcgtgcgtga aaatgtcaaa cggtttactt cgtttcatta cttcatactg ctgttcataa
     6721 ccctgtttgt tcacttgggc accattagag caccaattta cccgtttcag ggttgtagcg
     6781 aacacagcag aaatgctcat ttcatgggct tttctggcta gaaaaaaaat tttatcgctg
     6841 ccagatattt actatttttt attaagttgc tggagttcca ctaccatcag gaggagtagt
     6901 agagccacca ccattatttc caccattatc aggaggagta gtagagccac caccattgtt
     6961 tccgccatta tcaggaggag tagcagagcc accaccatta tttccgccat tatcaggagg
     7021 agtagtagag ccaccaccat tgtttccgcc attatcagga ggtgtagaac cgccaccatt
     7081 atcacctgtg ttattattgt tattattatt ttcttctggt tggttattgt ctggtttttt
     7141 tgtatcttca gatgatggtt gagaacttac tttagtagga gcagtacctt caataaatgc
     7201 tgctttagag ccagaagatt gctttcctcc atcctctaaa ttaatttttg taaaatatac
     7261 atttttagga acttcaaatt cagtaaccgt taaattagca tgtatttttt ccataatttt
     7321 ttcccatagt tttgcagtac tagtacttcc atgtggtact tctctacgtt ttatagtgtt
     7381 accagaatca tcttttctac cagcatcatc tccaatataa gttgcaccaa cataataagg
     7441 agtgtaaccg acaaaccaag catcatatgc actatttgta gtacctgttt tacctgctac
     7501 aggcattccc tttggaagtg cagcaacacc accagtacct tcagtaatta cactttctaa
     7561 catatctgta acgacataag ctacttcagg ttctacaact ttatgttcct caggagcatt
     7621 tttaactaat aattgaccat caaaggtagt tatagtagta aaaatagaag gttctacata
     7681 aataccgtga tttgccaatg taccataagc agcagccatt tgaagtggag taataccatt
     7741 cgccatacca cctagtgtca atgatgggaa ttttctatca cttgcttcag atgaacctga
     7801 gtgattattc tttaaagttg ttataccaaa gttctttaag taatccatca ttatatctac
     7861 acattcatca taagagtctc caagcatttc agcagttttg acagctatcg tgtttgaaga
     7921 tttagcaagt gctttacgta aactcataga accatgacca gcagtagtag ttcttggatt
     7981 ccatttatta ttttcttcaa acttatatcc tcctcgagaa tcacttagag cagttgcttg
     8041 tgttattttt agagtatcaa tagctggcgt atatattgct aaaggtttta tagaagaacc
     8101 tggctgatga gggttagtag cacgatttaa agtttttcta ccacttatat ttcttcctcc
     8161 agctagtgct cttatttcac catttttata atctaagatt accattgcag cttgcggttg
     8221 gctaactcca tcaggaccga ccatatgtcc tgggaaattg ctatttctat caaattcttc
     8281 ttctaaagca tcttgcattt tagggtctat agttgtgttt acaattagac caccattgta
     8341 gaataagttt tgagcttcat catttgtatg tccttgttcc attaaagcat ttataacttc
     8401 actttcgata aggtcttcta cagatgaaga aactttatct gatgattgag gcaattttat
     8461 ttctatttta gctttgattg cttcatcata ttcactttgt tttatatatt tcaattctaa
     8521 cattttcttt aaaacaattt cttgtctttt tttagcttca ggattactta cagcctttct
     8581 aacaactaat gtacctgctt ttaattgtct ataagtatca ttagatataa gctcccaaga
     8641 ttttattttt tcaatcatat caaagtccac ttgagtaggg tcatctaaat catcatcagt
     8701 tgtgttaaca aaaaataata atttattctc taaatcctcc ttagtttcat ttccctctaa
     8761 cttagaagtt ttataggctg aaaatcttga tggattttta gttgaaccag caagaagtgc
     8821 tgactctgct agagtaaggt cagctgcaga cttatcaaaa taaccacgtg caccagcctc
     8881 agcacctgct agacctctac caacgaagaa gttatttaaa taagtttcta gtattttatc
     8941 tttacttact gttttactca tctcatgtgc atagtaaata tcctttattt tacgaggtat
     9001 agtttgctgt tcagttgtta acagcatttt ggatacctgc ataggaatag tactaccacc
     9061 ttgcttagta cctgtaaggg tctttaatac tgacctaaat aaacctttaa tatcgacacc
     9121 cttatgctca taaaaacgct catcttctat tgcaattaca gcatgttgaa gataagggga
     9181 aatcttatct aatggaacgg gtgtcttttt atttacactt ggagctgtag ataatgtttt
     9241 tccattagcg tatttaatag tagttgtttg gttcgtctgt ttatctaaaa cagcttctgt
     9301 aactggagtt acatctctta gtgacgcaaa tactagacca gcagtacctg ctgtaccaac
     9361 aactagtaaa actaaaaata ctatcccaaa tactcttagt tttttaaatt tatcgctttt
     9421 tttagatttt ttcttgtttt ttgttgaacg agtcttgtta gctgaagttc tattaattgg
     9481 tttatttgta ttaccagatg agctaacttt ttttctcctg attttatttc catttttatc
     9541 attatcgtta ttcataagat tttaacctcc ttacttaatg caatattata acacaaatag
     9601 gagtagtttt gtaattaata gaaaaaatga atttgctata atattctgta cattgtatat
     9661 gtataaacgt aatatattaa aaagtctata tttttatttt atttattttt gacaaatatt
     9721 ataaaaatta tgccaaagct acatataaat aaataggagg ggataatttt gaatagaata
     9781 gtagatagaa aaacaaaaaa tgaatttaga aaaatcatag ctattttttt agtaattctt
     9841 ttcttgtctg tatttatagg cagttttata tatatagata aaactttaag accgacaata
     9901 acagttttag cagagactaa agctcttgaa ctagcaaata ggtcgataaa taaagctgtt
     9961 gctgagatgg tagaaggaaa aataaattat gaagatttaa tggatataca acttgataat
    10021 aatggtaaaa taactatgat acaagcaaac actataatga tgaatgaaat tgcatctgca
    10081 atagctttag aaatacaaga tgaattaaaa aaggataaaa cagcaagttc ttacatacct
    10141 ataggaacag cattaggaag tcctatatta gctaaatacg gtcctaaact tgaagtatct
    10201 attgaaccta taggaacagt atcagtaaac tttaaaacgg aatttgagtc ttcaggtata
    10261 aatcaaacta gacatagaat atatttagaa gctcaaacac aagttaaagt agtaatacca
    10321 cttataactt caacaaaaca aattaaagct caaataccta tttgtgaaac tataatagta
    10381 ggtgatgttc cagaaagtta tgtaaatatt ccagaaaaaa atctaggaaa tgttttacct
    10441 aacactggaa agaataccaa caagtaatat aaagttgtat atttttaaat aaatt
//
LOCUS       DAELLV010000127        10464 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.127, whole genome shotgun sequence.
ACCESSION   DAELLV010000127 DAELLV010000000
VERSION     DAELLV010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10464
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.127"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(153..1031)
                     /locus_tag="KPD58_002415"
     CDS             complement(153..1031)
                     /locus_tag="KPD58_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG0266177.1"
                     /translation="MRYDIAIIGSGPAGLSAAINAKIRNKTIIMFGNDNLSNKLVKAP
                     SIDNYLGFYDISGDELKDKFKSHIDSMDISIENKRINNIYAMGEYFTIMSGNDMYEAT
                     TVILATGVEYTRPIKGEEEFLGRGVGYCATCDAPLYRNKKVAVIGYNEESKEEANFLS
                     ELTSKTYFIPMYKKDNLMRSSDNLDDSIEVIHDRPVQIDGDKLVNKVSFKENHIEVDG
                     VFVIKDSTAPSALVPGIEIDGIHIKVDNNMKTSIDGCFAAGDCVGKPYSYIKAAGQGQ
                     IAALNAVYYLDKLKRA"
     gene            complement(1048..1365)
                     /gene="trxA"
                     /locus_tag="KPD58_002416"
     CDS             complement(1048..1365)
                     /gene="trxA"
                     /locus_tag="KPD58_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBG0266178.1"
                     /translation="MAKVINTSEFRSSVEGSKGVVVVDFFATWCGPCNMLGPVFAELG
                     EEMKDKARFVKVDIDESLEIAQQFNVSTVPTMIIFKDGKPVETLIGFMPKNKIEMQVK
                     SYL"
     gene            complement(1515..2747)
                     /locus_tag="KPD58_002417"
     CDS             complement(1515..2747)
                     /locus_tag="KPD58_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0266179.1"
                     /translation="MINKNVFTSTKNHLIKMYIIVVGSFLIIFSIFIYSYFRGLTYSG
                     IDSEINDELEYIVSQFKRTSFLNPIRLKDPKDMVYVYEDGRISYYTQNEYFDELLPDR
                     RLDKKNSFFKYTENGYTFRELNVDVGRYQIQIIRNIDSEMNSLRQLTSVLIIGILISV
                     IITYFVAVYLTRKALIPIETAWKNQAKFIQDASHELRTPITIVSSKLESMLKSPESTV
                     NDEVETIATAMKETRRLKKMITDLLSLTKEDSIVKVNLEEIDLEKLLEEISEDYIDIA
                     EFQEKRFVFNSKLKNKVIITDKNKLRQLILIFIDNAFKYTKLGDEIALELKEDIEDEV
                     TLLISDTGIGIKKEEIPLIFDRFFRSENVRNKDLEGSGIGLSIARMISLNLSIDINVT
                     SDVDIGTTFELSIPKKLK"
     gene            complement(2737..3408)
                     /locus_tag="KPD58_002418"
     CDS             complement(2737..3408)
                     /locus_tag="KPD58_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0266180.1"
                     /translation="MKVLIVEDNKILLESVVEELSKHFETEKCEDGEEALYLINQNIY
                     DLVILDLMLPNINGFDILKKMRINNIDTPVLILTAKETLDDKVEAFTIGANDYLTKPF
                     YMEELVARVYAILRTNGKIKERNGLEFKSLYLDTLEKRVYIEKEEIKLQNKQFNLLEY
                     FVLNKGSILLKEQIYDRIWGIDSDATIEIVEVYVSNLRKKLSKYGYDKYIKTKRKVGY
                     IFDDK"
     gene            complement(3424..4260)
                     /locus_tag="KPD58_002419"
     CDS             complement(3424..4260)
                     /locus_tag="KPD58_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266181.1"
                     /translation="MNKIVKKSISLMIILTIFIFMLTACEKDEQPDSVDIQTEDKNEI
                     KIDEGNAKVLNIGKSEIINIDEGDKVDTSTKVENNSTFNISNVELIYNEYDENKKIIS
                     SDSKALLDMTLMPGKVAYIECGHKTFVKGVEVYAYEYEAEGKIVYVNLKENTINIRNN
                     NIKLENSSQYEVLSTSELKKVNESNEGITYQVKVKNSSSKDLGNIILKTAKVNENGEY
                     LTVNRVPSYKILKPSEETDIDILCSTKAKSVEIVGYTYDDIKEKANVDIDLKSHKVKI
                     DK"
     gene            complement(4286..4960)
                     /locus_tag="KPD58_002420"
     CDS             complement(4286..4960)
                     /locus_tag="KPD58_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG0266182.1"
                     /translation="MNNNEDRIDKFLELQSKGFKFAEIAQNLKISQSTLRTFLNKKGY
                     KSEKGKYILKDELNYNKSLDNRNLEEEKAKEYEKSKKNTISNRKKVSTKKKISNEKAL
                     SKSVEQIEFNDTGLKNAKPKQNIKPKKDRKINITQEDLDKLCEVYDWYLEVKDYNIKP
                     KKVSNKKDVHIDDEHIDELKTTRIRIDKEIWNDFERLCSNSNFEKHQILTQALKEFMK
                     KYKNLL"
     gene            complement(4974..5702)
                     /locus_tag="KPD58_002421"
     CDS             complement(4974..5702)
                     /locus_tag="KPD58_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBG0266183.1"
                     /translation="MMFDRGKWNKLDYNELLEYMMSIQDIKYRDFNKKLIPGTENIIG
                     IRVPNLRKLSKEISQGNWKEFLEVAQDTYYEEVRLQGMVIGNINSNFEETLYYVKRFI
                     PKIDNWSVCDGFCSDLKSMKKYKENMYDILKKYVYSKNPWEIRFALVMFLIYYVDDKH
                     IKEIFEYCNNIQSEEYYVKMGMAWLLSICFIKCEEETFLYIKNNNLDDFTYNKMLQKI
                     IESNRVDLEKKNIIRSMKRKTRRC"
     gene            complement(5829..6518)
                     /locus_tag="KPD58_002422"
     CDS             complement(5829..6518)
                     /locus_tag="KPD58_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG0266184.1"
                     /translation="MDRYSEITNKNQREIVLLKGFPCIWGKCSFCDYIDDNSNLEEEM
                     NKLNLKVLKNVTGKYGVLEVINSGSCFELPKDTLEKIKCIIKEKNIKKLFLESHWSYK
                     NRLKEMREYFEIPVVFKIGVETFDNDFRNNILNKNANFKTPQDVKEYFDSPCIMVGIK
                     GQTKEMIDKDIEIILNTFEKATVNVFINNSSSIKRDEELVKWFVSKYKFLDENPNIEV
                     LYNNTDFGVGD"
     gene            complement(6526..7089)
                     /locus_tag="KPD58_002423"
     CDS             complement(6526..7089)
                     /locus_tag="KPD58_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG0266185.1"
                     /translation="MKRKICVKTITLIAFGISINIIGAFIAMGLRLPVYLDSIGTIMI
                     ASLLGPKYAVVTGVFGSLISGITFDVYSLYFAPVQISTGLLAGIAFKKNFLRGLKTPL
                     GVLLFAIPTSIISSIISAFLFGGMTSSGSSYIVQILKVLGLGDVFSVFVTQVFTDYGD
                     KLLAVVLVNLGLNAVPKTLKVSLTGGK"
     gene            complement(7091..8059)
                     /locus_tag="KPD58_002424"
     CDS             complement(7091..8059)
                     /locus_tag="KPD58_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HBG0266186.1"
                     /translation="MEKRKVIIDCDPGIDDSLAILLALNSPELEVIGITTCCGNVPAN
                     IGAENALKTLQMCSSLNIPVYIGEEAPLKRKLVTAQDTHGEDGIGENFYQKVVGAKAK
                     NGAVDFIINTLYNHEKVSIIALAPLTNIAKALIKDKKAFENLDEFVSMGGAFRIHGNC
                     SPVAEFNYWVDPHGADYVYKNLSKKIHMVGLDVTRKIVLTPNIIEFINRLDKKMAKYI
                     TEITRFYIDFHWEQEGIIGCVINDPLAVAYFIDRSICKGFESYVEVVEDGIAMGQSIV
                     DSFNFYKKNPNAIVLNEVDEKKFMYMFLKRLFKGYEDIIDSVEGVI"
     regulatory      complement(8160..8203)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            8569..9198
                     /locus_tag="KPD58_002425"
     CDS             8569..9198
                     /locus_tag="KPD58_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0266187.1"
                     /translation="MSIKERRKNEKEEMKKKIMDASIEIINQHGYENLSIRKIATKIE
                     YSPTTIYLYYKDKAEIISDMTNKLYNTVESNAIDIMNNCSSLPIDKQIIEIMTSFIKT
                     LSSEPEMAKSIMHSRMNIIFASENTNNTPSNNGIKMLDKFLSIGIEQGIFKKNIDKSS
                     WMLISALLGFVLCVVENKLYSLHNFSQLIENFLDILVGGLYNENSQQGY"
     gene            9173..9577
                     /locus_tag="KPD58_002426"
     CDS             9173..9577
                     /locus_tag="KPD58_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HBG0266188.1"
                     /translation="MKIRNKATNKKKKYVKISIALFFVFICIIIVIVSNLKPENLVVK
                     DIDINNVKNGIYTGSADNNLVKATVSVEVNNGKIQNINILKHDHLLGKPAEKITTSII
                     KQQSLDVDAITSATYSSNTIRKAVENALRKGE"
     gene            9580..10056
                     /locus_tag="KPD58_002427"
     CDS             9580..10056
                     /locus_tag="KPD58_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HBG0266189.1"
                     /translation="MNTIIIYSSKYGCTKDCANILKNKLSDNVTIVDINNNNNKIELS
                     KFDKIIIGSSIYVGSVSKKIQVLCNDNVELLNKKQVGIFLCCGFSEQADKYLKSNFPS
                     SLLESANAIGIFGSEARLEKMKFLDKLIMKAVSKGNYDSFRISQDNIDNFLINLNS"
BASE COUNT         3528 a         1716 c         1039 g         4181 t
ORIGIN      
        1 tataactttg agttttatta ataaaagtaa ttacatatag attttaaaat acttttatta
       61 ataacttaaa aataaaaaac tgtcttgaaa taataaaatt atttcaagac agttttttat
      121 aaatatcttt aaaaagttaa tcaatataaa tattaagccc tctttaattt atctaaatag
      181 taaacagcat ttaaagctgc tatttgccct tgaccagctg cttttatata agaatagggt
      241 tttccaacac aatctccagc agcaaagcat ccatctatac tagtcttcat attattgtca
      301 actttaatat gaatgccatc aatctctatt ccaggaacca atgcacttgg agccgtacta
      361 tcttttataa cgaatactcc atcaacttca atgtggtttt ctttaaaaga tactttgttt
      421 acaagtttat ctccatctat ctgtactggt ctgtcatgta taacttcaat agaatcatct
      481 aggttatctg aacttctcat aagattatct tttttgtaca taggtataaa ataagtcttt
      541 gaagtgagtt cacttaaaaa atttgcttct tctttagatt cttcattata tcctataact
      601 gctacctttt tatttctata aagtggtgca tcacatgtag cacagtagcc cacacctctt
      661 cctagaaatt cttcttcacc cttgattggt ctagtatact ctaccccagt tgcaaggatt
      721 acagtagtag cttcatacat atcatttcca gacattattg taaagtattc tcccatagca
      781 taaatattat ttattctttt attttcaatt gaaatatcca tactatctat atgtgattta
      841 aacttgtctt ttaattcatc accacttata tcataaaaac ctaaataatt atctattgaa
      901 ggagctttaa caagcttatt acttaaatta tcattaccaa acatgataat tgttttattt
      961 cttatttttg catttatagc agcagacaat ccagctggac cactgccgat tatagctata
     1021 tcatatctca taatactcac ccatttttta taaatatgat tttacttgca tttcaatttt
     1081 gttctttggc atgaatccaa ttaaagtttc tactggtttt ccatctttaa atataatcat
     1141 agttggtact gtagatacat tgaattgttg agctatttct aaactctcat ctatatcaac
     1201 ttttacaaat ctagctttat ccttcatctc ttcacctaat tctgcaaata caggtcccaa
     1261 catgttacaa ggtccacacc aagttgcaaa gaaatcaact acaactactc ccttactacc
     1321 ttcaacacta cttctaaatt cagatgtatt tataactttt gccattttta aatcctccta
     1381 ttaaacttta ttatccctat ataattatac tatgaaattt ataaatataa atttactttt
     1441 ataaggtctt tttatatttt atactaatta gtatacccat tcttcatttc taaaaacaat
     1501 tttttatatt tttattattt taactttttt ggtattgaca attcaaatgt agttccaata
     1561 tctacatcag aagttacatt tatatcaata gataagttta aactaatcat gcgagctatt
     1621 gaaagtccta ttccagaacc ttctaaatct ttatttctta cattttcact tctaaaaaat
     1681 ctatcaaata taagaggtat ctcttctttt tttatgccta taccagtatc acttataagt
     1741 aaagttacct catcctctat atcttctttc aattctaatg ctatctcatc acccaatttt
     1801 gtatacttaa aggcattatc aatgaaaatt aatataagtt gtcttaattt atttttatct
     1861 gttattataa ctttattctt cagtttagaa ttaaacacga atcttttttc ctgaaactca
     1921 gcaatgtcaa tatagtcttc tgatatttct tccaataact tttctaaatc tatttcttct
     1981 aaattaactt taacaataga gtcctcttta gtaagagaca ataaatcagt tatcattttt
     2041 ttaagtcttc tagtttcctt cattgcagta gctattgttt caacttcatc atttactgta
     2101 ctttctgggg attttaacat actttctaat tttgatgaaa ctattgttat tggtgttctt
     2161 aattcatgag aagcatcttg tataaactta gcttgatttt tccatgctgt ttctattgga
     2221 attaaagctt ttctagtgag atatacagcc acaaagtaag taattattac agaaattaat
     2281 attccaatta taagaacaga tgtaagctgt cttaaagaat tcatctcaga atctatattt
     2341 ctaattatct gtatttggta tctacctaca tctacattta attctctaaa agtatatcca
     2401 ttttctgtgt atttaaaaaa cgaatttttt ttatctagtc ttctatctgg aagtaactca
     2461 tcaaaatatt cattttgagt atagtaactt atccttccat cttcatatac atacaccata
     2521 tctttaggat ctttgagtct tattgggttc aaaaatgaag ttcttttaaa ttgagagact
     2581 atatattcaa gttcatcatt tatctcacta tcaattccac tatatgtaag tcccctaaaa
     2641 tatgagtata taaatataga aaagattatt aaaaaactac ctactacaat aatatacatt
     2701 tttattaaat gatttttagt actggtaaat acattcttat ttatcatcga atatatatcc
     2761 taccttacgt ttagttttaa tatatttatc atatccatat ttacttagct tttttcttag
     2821 attacttaca tatacttcca ctatttcaat agttgcatct gaatcaattc cccatattct
     2881 atcataaatc tgctctttta ataaaattga acctttattt aatacaaaat actcaagcaa
     2941 gttaaactgt ttgttttgaa gttttatttc ttctttttca atatacactc ttttttctag
     3001 tgtatctaaa tataaagatt taaattctaa tccgtttctt tcttttatct ttccattagt
     3061 ccttaatata gcgtataccc tagctactaa ctcttccata taaaaaggtt ttgtcaaata
     3121 atcattagct cctatagtaa atgcttctac tttgtcatct aatgtttctt ttgctgtcaa
     3181 tataagtaca ggtgtatcta tattattaat tctcattttc tttaggatat caaatccatt
     3241 tatattagga agcattaagt ctaaaatgac taaatcatat atattttgat ttattaagta
     3301 taaagcttct tctccatctt cacacttttc agtttcaaaa tgcttgctaa gttcttctac
     3361 tacactttct aaaagtatct tattatcttc aactattaaa accttcataa gtatccccta
     3421 cctttatttg tctatcttaa ctttatgaga ttttaagtca atatccacat tggctttttc
     3481 ttttatatca tcatacgtat atcccactat ttcaacactt tttgcctttg ttgaacacaa
     3541 tatatctata tctgtctctt ctgaaggctt taaaatctta tatgatggta cacgatttac
     3601 tgttaaatat tctccatttt cattaacttt tgctgtctta agaataatat ttcctaaatc
     3661 ttttgatgat gaatttttta cttttacttg atacgtaatt ccctcatttg attcatttac
     3721 ttttttaagt tctgatgtag atagcacctc atactgacta ctattttcta gcttaatatt
     3781 attatttctt atattaatag tattttcttt taaattaaca tacacaatct taccctctgc
     3841 ctcatattca tatgcataaa cttctacacc ctttacaaaa gttttatgcc cacattctat
     3901 gtaagcaact ttcccaggca ttaaagtcat gtccaacaaa gccttactat ccgaagatat
     3961 aattttttta ttctcatcat attcattata tatcaattca acattggata tattaaatgt
     4021 tgaattattt tctacctttg ttgatgtatc tactttatca ccttcatcta tatttataat
     4081 ttctgatttt cctatattca atacttttgc atttccctca tctattttta tctcattttt
     4141 atcttctgtt tgaatatcaa ctgaatcagg ctgttcatct ttttcacatg ctgtcaacat
     4201 aaaaataaat attgttaata taatcattag agaaattgat ttttttacta ttttattcat
     4261 attaagcacc tcatatagta gtattttata gtagattttt atactttttc ataaattctt
     4321 tcaatgcctg tgtaagtatt tgatgttttt caaaattcga attactacat aatctttcaa
     4381 aatcattcca tatttcttta tctatacgta ttcttgttgt ttttaattca tctatgtgtt
     4441 catcatctat atgtacatct tttttattag atactttttt aggctttata ttgtaatctt
     4501 ttacttctaa ataccaatca tatacttcac aaagcttatc taaatcttct tgagttatat
     4561 ttatttttct atctttctta ggcttaatat tttgttttgg ctttgcattt tttaaacctg
     4621 tatcattaaa ctcaatttgt tcaacacttt tacttaaagc cttttcatta gaaatctttt
     4681 ttttggtgct tacttttttt ctattactta tagtattctt tttacttttt tcatactcct
     4741 tagctttctc ttcttccaag tttctattat ccaaactttt attataattt aattcgtctt
     4801 ttaatatgta tttacctttt tctgacttat atcctttttt atttaaaaaa gttcttaaag
     4861 tactttgact aatctttaga ttttgagcta tctctgcaaa tttaaatcct ttgctttgaa
     4921 gttctaaaaa tttgtcaatt ctgtcttcat tgttattcat taatattcct ccgtcaacat
     4981 cttcttgtct ttcttttcat acttcttatt atattcttct tttctaaatc tactctattt
     5041 gattcaatta ttttttgtag cattttatta taagtgaaat catctaaatt attatttttt
     5101 atatagagaa aagtttcctc ttcacattta ataaaacaaa ttgatagcaa ccatgccata
     5161 cccattttaa cataatattc ttcactttgt atattattac agtattcaaa tatttcttta
     5221 atatgtttat catcaacata atatattaaa aacataacta atgcaaatct aatttcccat
     5281 ggattctttg aataaacata ttttttaagt atatcataca tattttcttt atattttttc
     5341 atagacttta aatcagaaca aaatccatcg caaactgacc aattatcgat tttaggtata
     5401 aatcttttta catagtataa agtttcttca aaattactat ttatattgcc aattaccata
     5461 ccttgtagcc taacttcttc atagtatgta tcctgtgcta cttctaaaaa ttccttccaa
     5521 ttcccttggc ttatttcttt tgataacttt cttaaattag gaactctaat acctattata
     5581 ttttctgttc caggtataag ctttttatta aaatctctat attttatatc ttgtatagac
     5641 atcatatatt ctaaaagttc attataatcc aatttattcc attttcccct atcaaacatc
     5701 atttaattgc tccttaattc cttttttctt attgtaacat aaaactctac aaaaaaagct
     5761 tctaactatt agaagctttt tcaaaaacat aagaaacttt tcaattttaa atttaaacta
     5821 aattaatgct aatctcctac accaaagtct gtattattgt ataaaacttc tatatttgga
     5881 ttttcatcca agaatttata cttactaaca aaccatttca ctaactcttc atctcttttt
     5941 atactagaag aattatttat aaaaacattc actgtcgctt tttcaaaagt atttaggata
     6001 atctctatat ctttatctat catctctttt gtttgtcctt ttattccaac cataatacaa
     6061 ggtgaatcaa aatattcttt tacatcttgt ggagtcttaa aatttgcatt tttatttaaa
     6121 atattatttc taaaatcatt gtcaaaggtt tcaactccaa ttttaaatac cacaggaatt
     6181 tcaaaatact ctctcatttc cttaagccta tttttgtaag accaatgact ttctaaaaat
     6241 aattttttta tattcttctc ttttataata cactttattt tttctaaagt atccttaggt
     6301 aattcaaaac agcttccaga atttatgact tcaagaactc catatttccc agttacgttt
     6361 tttaaaacct ttagatttaa tttattcatc tcttcttcta aattagaatt atcatctata
     6421 taatcacaaa aagaacactt tccccatata caaggaaatc ctttaagcag aacaatttct
     6481 ctttgatttt tatttgttat ctcactatat ctatccattt tctatctatt ttcctccagt
     6541 caatgatacc tttaaagttt ttggaactgc atttaatcct aaattcacta aaacaactgc
     6601 taacagctta tctccataat cagtaaatac ttgggttaca aacacactaa aaacatcccc
     6661 cagtcctaac actttcaaga tttgcactat atatgatgaa cctgaagagg tcatacctcc
     6721 aaataaaaat gctgaaatta ttgaacttat tatagatgtt ggaatggcaa ataaaagtac
     6781 tcctagtgga gtttttaaac cccttaaaaa attcttttta aatgctattc cagcaagtaa
     6841 tccggttgat atttgaactg gtgcaaaata gagagaatac acatcaaacg taattccact
     6901 tattaaactt ccaaacacac ctgtaacaac agcgtattta ggtcctaaaa gtgaagctat
     6961 cattatagtt cctattgaat ctaagtatac aggtaatctt aatcccatag ctataaaagc
     7021 gcctattata tttatagaaa taccaaaagc tataagtgta atagttttta cacatatttt
     7081 tcttttcaca ctatatcact ccttccacag agtctataat gtcttcataa cctttaaaaa
     7141 gcctctttaa aaacatgtac ataaatttct tctcatcaac ttcatttaga acaattgcat
     7201 taggattttt tttatagaaa ttgaaagaat ccactataga ctgacccata gctattccat
     7261 cttctacaac ttctacatat gattcaaatc ctttacatat acttctatct ataaagtatg
     7321 ctactgctag agggtcattt atcacacagc caattattcc ctcctgttcc caatggaaat
     7381 caatataaaa tctagttatt tcagttatat actttgccat cttcttatca agtctattta
     7441 taaactcaat aatattagga gtaagtacaa tttttctagt tacatctaaa cctaccatgt
     7501 ggattttttt agataaattc ttgtaaacat aatctgctcc atgtgggtct acccaataat
     7561 taaactctgc tactggagag caatttccat gaatcctaaa tgctcctccc atagatacaa
     7621 actcatcgag attttcaaat gctttcttat ctttaataag tgctttagct atatttgtaa
     7681 gtggtgcaag tgctattatt gatacttttt catgattata taaagtattt attataaaat
     7741 ccactgctcc attttttgct ttagctccta caaccttttg ataaaagttt tctccaatac
     7801 catcttctcc atgtgtatct tgagctgtta caagttttct ttttagtggt gcttcttctc
     7861 ctatatatac tggaatattt agtgaagaac acatttgaag tgtttttagt gcattttctg
     7921 cacctatatt tgctgggaca tttccacaac atgtggtaat tccaattact tctagctctg
     7981 gtgagtttaa agccagaaga attgccaaag aatcatcaat tcctgggtca caatcaatta
     8041 ttacttttct cttttccatt ttttatcctc cttgatttgt ggtcgaggag aactacacat
     8101 cttttttcca ctttcaaaaa ggtgtgtcta agggtattaa ttatatactg ccactatata
     8161 attccttagt tttttatact tggagttctc gaacaagtac agatgtaaaa tcatattttt
     8221 aggctactta ctataagact cttaaaaact atagaccgtt aaaaaatttt taaacatctg
     8281 atttaatagc tataaaataa attttatagc ataaaagaat ttattatatt ttaatgtatc
     8341 ttattaaagt ttatttagaa ttatgaacca actttaataa gatgtggcgc atttaaaccg
     8401 attattatta tacaataata tcttaaatat tacaattaat aacattaatt tattatccat
     8461 aaaaatatat taacttatat tatttaataa tttattgaca gtattaaaat taaattatac
     8521 aataacagtg taaattaact taacaccgtt aagcaaagag gtgattatat gagtatcaaa
     8581 gaaagacgta aaaatgaaaa agaagaaatg aagaaaaaga ttatggatgc tagcatcgaa
     8641 attataaacc aacatggata tgaaaattta tcaataagaa aaattgctac aaaaatagaa
     8701 tattctccaa ctacaattta tctttactac aaagacaaag cagagattat cagcgatatg
     8761 accaataaac tttataacac agtagaaagt aatgccattg acattatgaa taattgctca
     8821 tcactaccaa tagataaaca aatcatagaa attatgacta gctttataaa aactctttct
     8881 agtgaaccag agatggcaaa atctatcatg catagtagaa tgaatattat atttgcatct
     8941 gaaaatacaa acaatacgcc tagcaataat ggtataaaaa tgcttgataa attcctttcc
     9001 attggaattg aacaaggaat attcaaaaaa aatatagata aaagctcatg gatgcttata
     9061 agtgcattgt taggattcgt tttatgtgtt gttgaaaata aactatattc tttacataat
     9121 ttttctcaac ttatagaaaa tttcttagat attttagtgg gaggacttta caatgaaaat
     9181 tcgcaacaag gctactaata aaaagaaaaa atatgtaaaa atatctattg ctctattctt
     9241 tgtatttata tgcattatta ttgtaattgt aagcaactta aagccagaaa acttggtagt
     9301 aaaagatata gatattaata atgttaaaaa tggcatttat actggaagtg ctgataataa
     9361 cttggtaaaa gctactgtgt ctgttgaagt taataatggt aaaatccaaa atattaatat
     9421 cttaaaacac gaccatcttt taggtaaacc tgctgaaaaa attacaacaa gtattattaa
     9481 acaacagtct ttagatgtgg atgctataac ttctgcaact tatagcagta atactatacg
     9541 aaaagctgta gaaaatgctt taagaaaggg agaataatta tgaatactat tattatatat
     9601 tcaagtaaat atggttgtac aaaagactgt gcaaatatac taaaaaataa actatcagat
     9661 aatgtcacta ttgttgatat taataataat aataataaaa ttgagttatc aaaatttgat
     9721 aaaattatta ttggaagttc tatatatgtt ggttcagttt caaaaaaaat acaggtgcta
     9781 tgtaatgata atgtagagtt attaaacaaa aaacaagttg gcattttcct ttgctgtggc
     9841 ttttctgaac aagcagataa atatttaaaa tcaaattttc catcatcctt attagaaagt
     9901 gcaaatgcta ttggaatttt tggtagtgaa gctcgattag aaaaaatgaa atttttagat
     9961 aaacttatta tgaaggctgt ttcaaaaggt aattatgaca gttttagaat atcacaggat
    10021 aatatagata attttttaat aaatcttaat agctaaaata aagctaatta tttctatgat
    10081 aaattctttt aattataagt aatattgctc aaaatagtct ttaaacttta tcaaaatttt
    10141 atttttctta gtgaaatcta atgaaactag cacttaatat atgctttatt aatcataaca
    10201 aatatgtttt attaatcata acaaaaagag agtgtctaat gaactaaaaa attcattaga
    10261 cactctcttt ttgtatgaaa tcaaatatat ttttatcatt tcaacagaga aaaatacact
    10321 tgtctctatg tttggcacat tctcttttta taatataatg tttaatttaa aactactatt
    10381 tcaaatattg acatatactt aaactataga cttacaaaat caattcatat tagcttaaaa
    10441 tataatcttt gttattcaaa ttcc
//
LOCUS       DAELLV010000128        10200 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.128, whole genome shotgun sequence.
ACCESSION   DAELLV010000128 DAELLV010000000
VERSION     DAELLV010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10200
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.128"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            153..296
                     /locus_tag="KPD58_002428"
     CDS             153..296
                     /locus_tag="KPD58_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266190.1"
                     /translation="MSEFLLGVLASLTASFITYIISRKVKSHSGRSDFELDVKIKLNK
                     KQH"
     gene            804..1181
                     /locus_tag="KPD58_002429"
     CDS             804..1181
                     /locus_tag="KPD58_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0266191.1"
                     /translation="MKEEILIEKLLRAELIVMEYLWKKDTLMPKKEIVKEIKQIYGWH
                     KSTIKILLKRLIDKGYLARDIIKFQSYYKIIIDNEEYYAFKKKVLKSSKSRKIMRSLT
                     TTHKNVSKEKLDSLEEYYKNLKE"
     gene            1286..1669
                     /locus_tag="KPD58_002430"
     CDS             1286..1669
                     /locus_tag="KPD58_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0266192.1"
                     /translation="MKISKLPEAELKVMRYIWEANKVLTSRELVAAMEEKYEWNESTT
                     FTVLKRLERREFLSTEKIGKLTHYNILVKEKKYLRFETKEFLKNIHKNSISSLISALH
                     SKDDEVDEDKLIELEEHFKNLKEKE"
     gene            1854..2438
                     /locus_tag="KPD58_002431"
                     /pseudo
     CDS             1854..2438
                     /locus_tag="KPD58_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896033.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            2464..2877
                     /locus_tag="KPD58_002432"
     CDS             2464..2877
                     /locus_tag="KPD58_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0266193.1"
                     /translation="MKKIILASHGDFSKGLLNAVKMIVGDLADCVSSYGLYPGQSASD
                     FALELEKTISEDKECEYIILSDLYGASVCTAMLRLTNLHNVRLFSGMNLNMVLELLTR
                     FSDELTVEDIDQLVEESRRGIQSVTLQIKEEEEVF"
     gene            2881..3624
                     /locus_tag="KPD58_002433"
     CDS             2881..3624
                     /locus_tag="KPD58_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane biogenesis protein"
                     /protein_id="HBG0266194.1"
                     /translation="MKKKGILSVFKSKKPIIAMIHLKGDTPEDIFERAKKEITIFEEN
                     GVDGIMLENYYGNYYDLERILEYVSKANLSIPYGVNCLNVDTMGFELATKYNASYIQV
                     DSVVGHVKPRDEATLEEFFKLQRSKCPAYLIGGVRFKYQPVLSENDVEEDLKIGMTRC
                     DAIAVTENATGQETSMEKIELFRKNLGDFPLVIAAGVTLENAKKQLKLGDMAIIGSYF
                     KDNYKDFGDVSVEHVKTFMDEIKKIREEL"
     gene            3624..4094
                     /locus_tag="KPD58_002434"
     CDS             3624..4094
                     /locus_tag="KPD58_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0266195.1"
                     /translation="MIKLVRVDHRLIHGQVAFTWTKFLSTDCILIASDDLLKDELRMA
                     GLKMAKPSNVKLVMKSIADSIKALNSGVTDKYNLLILCESVEDVYRLAKEVKAIKSIN
                     LGGTKSDDNRENISKAVHVSKDDIKMIKELDSEGVNVFVQLVPDDDATNVMKLI"
     gene            4111..4869
                     /locus_tag="KPD58_002435"
     CDS             4111..4869
                     /locus_tag="KPD58_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0266196.1"
                     /translation="MEFTQVILITLIAFFAYMHSFVGSTMHNRPIVVAPLVGLALGNL
                     HTGIVIGSTLELVFMGAFPVGASNPPDFVSGSIIATAFVILTGQDVSAAVVLAVPIAT
                     LVLLIDNFLMTVVLTWGAHIADRYAEEGNIEGVERVQLLFGIGNKLILAIIVGIGFSL
                     GVPVIEKILSFIPSYVTHGMDVAAGVIPAIGFAMLARMMLNKKTVAFLLLGFILVAYL
                     NITVTGVALFGLAIALIYVNFVGEKEVIVDDNEF"
     gene            4853..5695
                     /locus_tag="KPD58_002436"
     CDS             4853..5695
                     /locus_tag="KPD58_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG0266197.1"
                     /translation="MTTSSKKLETISPDSKITRKDFWKCFRRSLTLDSSWNYERMQNI
                     AYAYMMAPIIRRLYKDDKEKKSKALKRHLEFMSVTPHISTLLVGISGAMEEENAKNKE
                     FDANSINAVKSSLMGPVSGIGDSFFWGTLKLIAAGVGIALASQGNIMGPILFLLIINV
                     PHFIIRYICLDKGFKYGTQFFKDVSGSSIVSKVMEAASMLGLMVIGGMTASNVMLKLS
                     VNVGSGEWAEPIQTYLDQIMPCMLPAMIFGIMYWLLGKKVKTTTILISVMIICIVLAA
                     IGVV"
     gene            complement(5855..6760)
                     /locus_tag="KPD58_002437"
     CDS             complement(5855..6760)
                     /locus_tag="KPD58_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG0266198.1"
                     /translation="MDFKQLEVFVAVAKHQSFSKAARELFLTQPTVSAHIQNLERELE
                     TVLINRSNKVITLTKSGEILYEHAIYILNNCKRAIYDIKEYSGKIEGIIDIACSSIPE
                     TYILPDFMKSFSMSYPDVKFSISHYDSQYAISEILNERISFGLVGSKINNPQIEYLDL
                     LDDELVLITPSDFKIDNKNNCIDIGELAYLNFIMRKEGSGTRNLILNTLSKNNFPVSK
                     LNVIAHVESNEAIKEMVRLGLGVSFISYISAIDYLNAGKIKCYKIKDVDFTRKFFFIY
                     SKKKTFSPLEDKFLNRLCEYFEIII"
     gene            7122..7856
                     /locus_tag="KPD58_002438"
     CDS             7122..7856
                     /locus_tag="KPD58_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266199.1"
                     /translation="MKLKRSLVCVTILGIILVGCHKENTKEKNQVASKATQQKTMTKV
                     QNDVNEIMNKDYKYIIKNMGIPYNTFYYIKPKVLKESNTMQDINTSSYMTLVYPKYTG
                     NDELDGSALYVDINENKVVNVETNSFSSQGISVIDAESSIVIEKSDHEKSAVSLENFR
                     HIDLGEYVGVEDSRINEIVGDANYDLTAYNHEGSKVVKSYRLKEDNKILKKEVLTISI
                     VDNKIKSIKTIESDKIVKIIKGTLLE"
     gene            8067..9248
                     /locus_tag="KPD58_002439"
     CDS             8067..9248
                     /locus_tag="KPD58_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HBG0266200.1"
                     /translation="MIELEDAIKIIEKNVLPINRVKIVNLIDAVNKVVAEDIYSTIDS
                     PPFDRSPYDGYAYNANSENRNLKVIGTLYAGDVFNRVLNKNEAVKIMTGGKIPQGANC
                     VIKKEDVTVIGDMITLNIKLKEYDNYIFKGEDIKKGQLIINKNQNIGYDGIGVLASLG
                     ISKVTVYDDLKVGILNTGTELQDINETLENGKIYDSNRYTLYSRLLKLGIQADTICNC
                     SDDILDLEKKVKNMLQSVDFLITTGGVSVGDKDLIPDVFKKIGATELFWKINIKPGGA
                     CYVGTLGEKLLFGLSGNPHAAIVVFDNVIVPVIEYLTYKKGTHHIKAIFKGEFNKLSN
                     KDRFVSGKLYTSEGKLYVELNDKKDAKARLSATLRSNCMIKIKKNETIKENQLVDVVI
                     R"
     gene            9232..9804
                     /gene="mobB"
                     /locus_tag="KPD58_002440"
     CDS             9232..9804
                     /gene="mobB"
                     /locus_tag="KPD58_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HBG0266201.1"
                     /translation="MLLLDSFMKGHNFMYNILSVVSYSGVGKTTLIEGIIKELNKKGY
                     RVGVIKHTCHDFDMDEEGKDTYKHRKSGARKVCIISDKRVAYIEELKEKNPLYKMIQL
                     YEDMDLIIIEGYKNYRFKRLEVTRKGKYEDILSNKSDLIGIVSDIEYKLNIEQFNLND
                     YKNISKYIESCIKNEILSISNVEIKNILKE"
     gene            9923..10198
                     /locus_tag="KPD58_002441"
     CDS             9923..10198
                     /locus_tag="KPD58_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0266202.1"
                     /translation="MKKILVACGAGIATSTIVCDRVERLVKENNIQAEVEQCKIAECS
                     TKQEGADLIVSTTILPTTYDIPTIKATGYITGINTTALDKKILNALK"
BASE COUNT         4107 a         1043 c         1741 g         3309 t
ORIGIN      
        1 ttgtataaat aaaaaatatg aagaatagat atttgataat tgtaaaatta tgttataatt
       61 aaaatgcaag aatatatctt gtaagaaact acaatccaag agtggagctt cattttctac
      121 attccatccc ttaaaaggaa ggaggtggag aaatgagcga atttttacta ggagtgttag
      181 ctagtttaac agctagcttt attacatata ttatttccag aaaagtaaaa agccactctg
      241 gcaggagtga ctttgagctt gatgtgaaaa tcaagcttaa taaaaaacaa cattaatatt
      301 tagttaatga aacttcactc taggctaata gattgtagtt tctttttttg tttttgatac
      361 gaatttacat ctttattata tcgcattttg agaaaaaata aaactatgaa tattagaaat
      421 atattttatt ttatctctaa gtatatatat tattagccac ctaaaaaact ttcacaaaaa
      481 caaaaagagt atatgttttt gataactgga tgatataact tataactcat tttacaattc
      541 ttagttcata tatatttatg ccactaaatt tctctagttt tgccgattta gttttattct
      601 acaactaaaa taatacaata gaattagtta ttattcaaca agattgtttg aaagttaaat
      661 aaatttatag atatgaacat tattttacac ttaaaaaaac agactaaata tgtagaatat
      721 ttggtaaaac ttacctctag tttttgtctt aaaaagctcc tattattaat ttaagttaga
      781 cgaatttaag gaagtgacat aaattgaaag aagaaatatt aatagaaaaa ttattaagag
      841 cagaattaat agtaatggag tatttatgga aaaaggacac tctaatgcct aaaaaagaaa
      901 ttgtaaaaga aataaaacag atttatggat ggcataaaag tacaataaaa atactactaa
      961 aaaggctaat tgacaaaggt tatttggcta gggatattat aaaatttcaa tcttattata
     1021 aaataataat agataatgaa gaatattatg cttttaagaa aaaggtacta aaatctagta
     1081 aaagtagaaa aataatgcgt tcacttacca ctacgcataa aaatgtaagt aaagagaagt
     1141 tagactcatt agaagagtac tataaaaact taaaagaatg atatggtaaa aattggatgg
     1201 aattatttac cttaattctc ttgtttgtaa aactttattg atatagaatt taacatatac
     1261 ataaatcggt aaaggggtgt agactatgaa aattagtaaa ttaccagaag cggaattaaa
     1321 agttatgaga tatatttggg aagctaacaa ggtattaact tctagggaat tagttgctgc
     1381 aatggaagaa aaatatgaat ggaatgaatc aacgacattt acagttttaa aaaggttaga
     1441 gcgaagagaa tttttaagca cagagaaaat aggcaaactt acacattaca atatactagt
     1501 aaaagagaaa aaatatctaa ggtttgaaac gaaagaattt ttgaagaaca tacataagaa
     1561 ttcaatttca agtttaatat cagcgttaca tagtaaagat gatgaagtgg atgaagataa
     1621 attaattgaa ctcgaagaac attttaaaaa tttaaaagag aaagaataaa taaagattat
     1681 tttaggtggc aaattatttt tgccaccttt tttgccacca tgacagttta ataaaataaa
     1741 atacaaataa agaaatataa ataaaataga ataaagaaat tgttttagat aggttgtaaa
     1801 taaatgtact cataaaaaca taaataaata taaataaaca taaataaaat ataagaattt
     1861 ctgtgagaaa tgcacttaat gaacttgtaa aagaagatat attatgtaaa aaacgtggta
     1921 aagggactta tgtaacaata ccagagagaa tagaagcaac atgtgcagga aatagtttta
     1981 ctaattcttg tcatcgtatt aatgcaaaac caagtaccaa aataatctct gttttaataa
     2041 aaaaagcaga taagcaagta gctgaagctt taagtattga actagaagag aaggtaatat
     2101 gtattaaaag acttagatta atagatgaag tacctgttat ttttgaagtt gactatttta
     2161 gaatagatta catgttttta ttaaaagaag aattagaaga taaatcttta atggaggtta
     2221 tatctaataa tataagcaca ttacctaaac gagtagaaaa tatatttgaa gtaaaacatt
     2281 caaacaaaga atattctgac catcttaaat gtgcaagtaa tatgccacta ttgaaagtaa
     2341 gacaatcggt gtacacagaa aataatgatg tcttatatta caatgagcaa tttataagaa
     2401 gtgataagta caaatatgca gtatcagcag aaatataatc tataatttag taggaggatg
     2461 agtatgaaaa agataatatt agcatctcat ggagactttt caaaagggct tttaaatgca
     2521 gtaaaaatga tagtaggaga tttagcagat tgtgtcagtt cgtatggttt gtaccctgga
     2581 caaagtgctt cagattttgc tttagaatta gaaaaaacaa tatcagaaga taaagaatgt
     2641 gaatatataa ttttatctga cttatatgga gcaagtgtat gtacagcaat gttaagatta
     2701 actaatctac ataatgttag gttattttct ggaatgaatt tgaatatggt attagaactt
     2761 cttacaaggt tttctgatga attaacagtt gaagatatag accaattagt ggaagaatct
     2821 agaagaggaa tacaaagcgt gacattacaa attaaagaag aagaagaggt attttagttt
     2881 atgaaaaaaa agggaatatt atcagtattt aaaagtaaaa aaccaattat agcaatgata
     2941 cacttaaaag gagatactcc agaagatatc tttgaaagag ctaaaaaaga gattacaatc
     3001 tttgaagaaa atggtgtaga tggaataatg ttagaaaatt actatggtaa ttattatgat
     3061 ttagaaagaa tacttgaata tgtatcaaaa gctaatttat caataccata tggagtaaac
     3121 tgtttaaatg ttgatactat gggatttgaa ctggcaacaa aatataatgc tagttatata
     3181 caggttgatt ctgtagtagg tcatgttaaa cctagagatg aagctacttt agaagagttt
     3241 tttaaattac aacgttcaaa atgcccagcg tatttaattg gaggcgtacg ttttaaatat
     3301 cagccagtat tatcagaaaa tgatgttgaa gaagatttaa aaataggaat gacaagatgt
     3361 gatgctatag cagttacaga aaatgctaca ggtcaagaaa catcaatgga aaaaatagaa
     3421 ctgtttcgta aaaatttagg agacttccca ttggttatag ctgctggagt tacattggaa
     3481 aatgcaaaaa aacagcttaa attaggagat atggcaataa taggaagtta ctttaaagat
     3541 aattacaaag attttgggga tgttagtgta gaacacgtaa aaacattcat ggatgaaatc
     3601 aaaaaaatta gggaggaatt ataatgatta aattagtaag agtagaccac agacttatac
     3661 atggacaagt agcatttaca tggacaaagt ttttaagtac agactgtata ttgatagcaa
     3721 gtgatgactt attaaaagat gaattgagaa tggcaggact taaaatggct aaaccatcta
     3781 atgttaagtt agtaatgaaa agcatagcag attctataaa agcacttaac tcaggtgtta
     3841 ctgataaata caacttattg atactttgtg aatctgtaga agatgtttat agacttgcta
     3901 aagaggtaaa agctattaaa tctataaacc ttggtggaac aaaatcagat gataatcgtg
     3961 aaaatatatc taaagcagtg catgtatcaa aagatgatat aaagatgatt aaagagttag
     4021 attcagaagg cgtaaatgta tttgtacaat tagttcctga tgatgatgct acaaatgtaa
     4081 tgaaattaat ataattttag ggggatgaaa atggaattta cacaagttat tttgataact
     4141 ctaattgcat tctttgcata tatgcatagt tttgtaggtt ctacaatgca taatagacct
     4201 atagttgttg caccacttgt tggtttagca ctaggtaatc ttcatacagg tatagtaata
     4261 ggttcaacat tagaattagt atttatgggt gcatttcctg ttggggcaag taatcctcct
     4321 gattttgtgt ctggttctat tattgccaca gcgtttgtaa tattaacggg tcaagatgta
     4381 tcagcagcag ttgtacttgc agttcctata gcaactctag tattgcttat agataacttc
     4441 ttaatgacag tagtacttac atggggagct catattgctg accgttatgc agaagagggg
     4501 aatattgaag gcgtagaacg tgtgcaatta ttatttggta taggaaataa attaatatta
     4561 gcaataatag taggtatagg attttcatta ggtgttccag taatagaaaa aatactttca
     4621 tttataccat catatgtaac tcatggaatg gatgttgcag caggcgtgat tccagcaata
     4681 ggttttgcca tgttagctag aatgatgctt aataagaaaa cagtagcatt tttattatta
     4741 ggatttatat tggttgctta cctaaacata acagtaacag gagttgcatt gtttggatta
     4801 gcaatagctt taatatatgt taactttgta ggagagaagg aggtaatagt agatgacaac
     4861 gagttctaag aaattagaga caatttcacc agatagtaaa ataacgagga aagacttttg
     4921 gaaatgtttt agaaggtcat tgacattaga ttcatcttgg aactacgaac gtatgcaaaa
     4981 tatagcatat gcatacatga tggcaccaat aatccgtaga ttatataaag atgataaaga
     5041 aaagaaatct aaggctttaa aaagacattt agaatttatg tcagttactc ctcatataag
     5101 tactcttttg gtaggtatat ctggagctat ggaagaagaa aatgcaaaga ataaagaatt
     5161 tgatgctaat agtataaatg ctgttaaatc aagtttaatg ggtccagttt ctgggatagg
     5221 agattcattt ttctggggaa cattaaagct tatagcagct ggggttggta tagctttagc
     5281 ttctcagggt aacataatgg gaccgatttt attcctttta ataataaatg ttcctcattt
     5341 tattatacgc tatatatgtc tagataaagg atttaagtat ggaactcaat tctttaaaga
     5401 tgtgagtggt tcaagtatag tttcaaaagt tatggaagca gcttcaatgt tagggcttat
     5461 ggtaattgga ggtatgactg catctaatgt aatgttaaaa cttagtgtta atgtaggtag
     5521 tggagaatgg gcagagccta ttcaaactta cttagaccaa ataatgccat gtatgcttcc
     5581 agctatgata ttcggtatta tgtattggtt attaggaaaa aaggtaaaga caactacgat
     5641 attaatttca gttatgatta tttgtatagt attggctgct attggagtag tataaataaa
     5701 aaaatcccaa gttcatttga acgagggatt ttttgactaa attaaataaa aaagaaagat
     5761 ggttcataat aatttataag catttatgtg atagttataa ttagcttatt aattagttat
     5821 aaactcaaaa ttaagcacta ctttattaaa gttattatat tataatttcg aaatactcac
     5881 ataatctgtt tagaaattta tcttctaatg gggaaaaagt tttcttttta gagtaaatga
     5941 agaaaaactt tctagtaaaa tcaacatcct ttattttata acattttatt ttacctgcat
     6001 tgagataatc gatagctgaa atgtacgata taaaggatac tccaagacct aatctaacca
     6061 tttccttaat agcctcattt gattctacat gagcgattac atttaattta cttactggaa
     6121 agttattttt actaagtgtg tttagtatta agtttcttgt acctgaccct tcttttctca
     6181 taataaaatt tagataagcc aattctccta tatcgataca attattttta ttatcaattt
     6241 taaaatctga aggagtgata agaacaagtt catcatctaa taagtctagg tactctattt
     6301 gaggattatt gattttagaa ccaactaatc caaagcttat tcgttcattt aaaatttctg
     6361 atatagcata ttgagaatca taatggctta tggaaaattt tacatctgga taactcatag
     6421 aaaaactctt cataaaatca ggtaaaatat aagtttcagg tatggaacta catgctatat
     6481 ctatgatacc ttctattttt cctgaatatt cttttatatc ataaattgca cgtttgcaat
     6541 tattcaatat atatatagca tgttcataaa gaatttcacc agattttgtg agtgtaataa
     6601 ctttgttgct tctatttata agaacagttt ctagctctct ctctaaattc tgtatgtgtg
     6661 cgcttacagt aggttgagtt aaaaaaagtt ctcttgcagc ctttgaaaaa ctctgatgtt
     6721 ttgcaacagc aacaaatact tcaagttgtt taaaatccat tatttaatcc tccttaaatt
     6781 atctaaaact attataacat atttgataaa ttaatagtca aaggtgattt ttaaaataga
     6841 aaacctttaa atattcatgg agtttttgat tgtttaggaa taatttggta taatgttgta
     6901 aggtacttac aataaaaata tcgtaaattg aattttcaaa attcaacttt aaagtgtttt
     6961 ttattacaga aaaattacaa ttagcttctt aatcttatta aattcaaata aatattatat
     7021 aattatttta taatataata aatataatta ttgaaaaaag agataactgt aaattgagat
     7081 atagataaat actataaaat ttaagaaagg aggattgatt tatgaagtta aaaaggtcat
     7141 tagtttgtgt aactatttta ggtattatac ttgttggatg tcataaagaa aatactaaag
     7201 agaaaaatca agtagcaagt aaagcaacac agcaaaaaac tatgacaaaa gttcaaaatg
     7261 atgttaatga aattatgaat aaagattata agtacattat aaagaatatg ggtatacctt
     7321 ataatacatt ttattatata aaacctaaag ttttaaaaga atcaaataca atgcaagata
     7381 taaatacatc aagttatatg acattagttt atccaaaata tactggaaat gatgaactag
     7441 atggaagtgc tttatatgtt gatattaacg aaaataaagt tgtcaatgtt gaaaccaatt
     7501 ctttctcatc acaaggtata tcagtcattg atgctgaatc tagtatagtg attgaaaaaa
     7561 gtgaccatga aaaatcggct gtatcactag aaaattttag acatattgac ctgggagagt
     7621 atgttggtgt tgaagattcc agaataaatg aaattgtagg agatgcgaat tatgatttaa
     7681 cagcatataa tcatgaaggc agtaaagtag ttaagagtta tagattgaaa gaagataaca
     7741 aaatattaaa aaaagaggta cttacaataa gtattgtgga taacaaaata aaatctataa
     7801 aaactattga aagtgataaa attgtaaaaa taataaaagg aactttatta gaataaaata
     7861 ttctaaagca tatgatgctg tttcaagata gaaggaaatt tctagctggg aacagttttt
     7921 ttattagatt tataaaattt gaactgcact tatagataac tattagttat aattaaatat
     7981 aagataaaag tatttccaac ttttataatc tataatatat tacatagaaa tatcttaaat
     8041 aaaaaatata ttggggagta tttaaaatga tagaattaga agatgccata aaaattattg
     8101 aaaaaaatgt tttaccaata aacagggtca agatagtaaa tcttatagat gcagtaaata
     8161 aggtagttgc agaagatata tattcaacaa ttgatagtcc tccatttgat aggtcaccct
     8221 atgatggtta tgcatataat gcaaattctg aaaatagaaa tttaaaggtt atagggactt
     8281 tgtatgcagg agatgtattt aatagggtat taaataaaaa tgaagcagtg aagataatga
     8341 ctggtggaaa gataccacag ggtgcaaatt gtgtaataaa aaaagaagat gtaacagtta
     8401 taggtgatat gataacttta aatataaaat taaaggaata cgataattac atatttaagg
     8461 gtgaggatat aaaaaagggg caattgatta ttaataaaaa tcagaatata ggatatgatg
     8521 gtattggagt attagcaagt cttggaatat ccaaagtaac tgtttatgat gatttaaaag
     8581 ttggaatttt gaatacaggt acagaactac aagatataaa tgagacttta gagaatggta
     8641 aaatatacga tagtaataga tataccttat attcaagatt attaaaactt ggaatacaag
     8701 cagatacaat atgcaattgt tctgatgata tattggattt agaaaaaaag gtaaagaata
     8761 tgttacagag tgtagatttt ttaattacca caggtggtgt ttctgttgga gataaagatt
     8821 taattccaga tgtattcaaa aaaatagggg caacagaatt attttggaag ataaacatta
     8881 aacctggtgg agcttgttat gtaggtacat tgggagaaaa gctattattt ggtctttctg
     8941 gtaatccaca tgcagcaata gtggtatttg ataatgtgat agttcctgtt attgagtatt
     9001 taacatataa gaagggaaca catcatataa aagctatatt taaaggagaa tttaataaac
     9061 tttcaaataa agatagattt gtaagtggaa aactatatac aagtgaaggt aaattgtatg
     9121 tagagctaaa tgataagaaa gatgcaaaag ctagactatc tgcaactcta agaagtaatt
     9181 gtatgattaa gattaaaaag aatgagacaa taaaagaaaa tcaattagtg gatgttgtta
     9241 ttagatagtt ttatgaaagg acacaatttt atgtataata tattgtcagt tgtctcatat
     9301 tcaggagtag gtaaaactac gttgattgaa ggcataataa aagaattaaa taaaaaaggg
     9361 tatcgtgttg gagttattaa acatacctgt catgattttg atatggatga ggaaggaaaa
     9421 gatacttata aacatagaaa atctggggcg agaaaagtct gtataatatc agataaacga
     9481 gtagcatata ttgaagaact aaaagagaag aatccattat ataaaatgat tcaattatat
     9541 gaagatatgg acttgattat catagaagga tataaaaatt atagatttaa aagattggaa
     9601 gtaactagaa aaggtaagta tgaggatatt ttatctaata agtctgattt gataggtatt
     9661 gtcagtgata tagaatataa attgaatatt gaacagttta atttaaatga ttataaaaat
     9721 atatcaaagt acatagagtc ttgtataaaa aatgaaattt taagtataag taatgttgaa
     9781 ataaaaaaca tattaaaaga ataattttaa gatgttcttc ttatataaaa tgatataatt
     9841 atttatataa ataaagatat aagaaattta tcaattttat tttgagttat aaaatattaa
     9901 agaaattgta ggaggacaat atatgaaaaa aatattagta gcctgtggag caggaatcgc
     9961 aacatcaaca atagtttgtg acagagtaga aagattggtt aaagaaaata atatacaagc
    10021 agaagttgag caatgtaaaa ttgctgaatg ttcaacaaaa caagaaggtg ctgatttaat
    10081 cgtatctact actatactac caacaactta tgatatacct acaataaagg caacaggata
    10141 tataacaggt ataaatacaa cagcattaga taaaaagata ttaaatgcat tgaagtagtt
//
LOCUS       DAELLV010000129        10199 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.129, whole genome shotgun sequence.
ACCESSION   DAELLV010000129 DAELLV010000000
VERSION     DAELLV010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10199
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.129"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(90..1970)
                     /locus_tag="KPD58_002442"
     CDS             complement(90..1970)
                     /locus_tag="KPD58_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp10"
                     /protein_id="HBG0266203.1"
                     /translation="MSKRKSFIRTIAVSTMAVAVTGSATCAYAAPVLQGTKTYEKVNT
                     IDISVDSVENIVYSFQASIKVQGEVEVVDNEQKEKITWSDNIKSQIKSGNANATCRVE
                     YNKSSNTTTLDIYVTSNEDLLDGNRLNIGRISVKKSGSNSNADYKVLGKGTNDKPALK
                     IVTYNNKTVDYENISSDEGLIFTLINESEVKPIGGTGSSKNDPEKYKVEKSEALEYLL
                     NNIRINYSIVSKETQESGSNVILKLGLAQKTTKGRKATINKYVEVTLPKSLEYIVENE
                     LSKPDELPPDNGNGGNNGGGSNSGGSSSGGSSGGGSSSDSTSNVTVKKLKGADRFETA
                     IKISQSGWTKSDTVVIVNGEDKSMVDGLTATPLASVKNSPILLSSNEKLPQKTVEELK
                     RLNPSKVIVIGGNNSMPNSVVEAIKAVNSKISVQRIGGDTRYQTSINIAKEIDKTNNV
                     SKLYIGAGNGEADSLSIASLAGKEKTPIVLTQKDGVDNEAEQFIKSNKVSNIYFIGGV
                     EKISNKAIEQVGKIANKDISNNRVAGQTRQETNAKVIDKFYSQSKLDGVVVANQDKLI
                     DALAVGPLAAKNNSPVILATNTLDKSQESSLKGKNSSKLFEVGGGIASSVIDKIKSLI
                     EK"
     gene            complement(2174..8137)
                     /locus_tag="KPD58_002443"
     CDS             complement(2174..8137)
                     /locus_tag="KPD58_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding adhesion protein"
                     /protein_id="HBG0266204.1"
                     /translation="MKKAISCVLAVSMCSAPLNVFAEPILEGKLRAVEKSVTEKLRGS
                     LEVDLNFSLPIKNSESESMTNMQLRLKDDSNNSGIIKLGEKDSGTVNVGTDNIEYTIK
                     KLSASRSEIKNKDENISYYNIVFNNLPVGKYNVEVSGDGFKNKEIKDIDISSYSQRVL
                     LSNRASVDNKNKPLNYELFLMGDVNGDGKVDKSDYNKVLENIDSNKREFDLNRDGKVD
                     IVDLDYVQKNLGQSEDAKSLESIVSTNPIVDTSKVELKGNSDVDINGNVENLFSGENN
                     GVTISSKNDEISEEKPAVMDIALSEALNMEVINIKAPEGTAPAKGIVEVTDKDGNVQQ
                     IPYENKEYMKNLKDEEQQEEVNNEQEEVNQEEVNNKDENINQTDNNSNNKDENNDKQQ
                     ENSDKQEANSNTNQDKNNDKENSSMDKPNSDEDTPKEEGNISKDDNSTTSTYSRSALE
                     TYADVQNSDIVINLGKQVAVKKITIKITATTANDRNLAEISKVEFLNNVYKEIPKPEM
                     NIPKINKISTSTAVGNEKVSFYWNNEVNVSAYELILDKIDSKGKVLSTKKLQTSKNTI
                     EVRDIEAYGLYRVSIQSLNGDWSSGYKDATPENLDGVPENVDSNYKPIQFSPDSILEF
                     QVVPDTKPEPPEGINVKGKFKSLDVSWKNHKQAKDFDLYYKEKGSNGSWIKYNDKLIR
                     GTSSKIEGLKDNTTYEVRMTATNHLGTSGMSKTYIGTTESMDAPTTPNYRLINTPKEN
                     GGFGIPTNHIVDVVYPDGYDKSEYSDKNPFDKFNVVDGDFTTHWTFPTYNAAEGTNRG
                     PIITFDKSYTMDTFMATPRFDVSDKTINDFEVRYWDENGQLHNLGDVPFETKTDPVNN
                     NKYVMVRLKEPITATKIQVSVCSSYSYKSNISELKFYEYDSIEDDVRNLFMDGLQVEL
                     KEDVTQEKITELKDRLNTPDKASGEYHPYKTVIERELKLAQDLYNDRATLTDVTTVNQ
                     EINKLGKYKDKNGQYTVNSNNLGMQNDWQALGVAARAGDEITVYVGSKSGKTPKLIYT
                     QYYGESGAYKSGEINLNTGKNVIQLSKLHSLDIECGGSLYIRYAEDTPSGGDIKVRVA
                     GATKIPHLNLNGTINDKSPAGVSESKKKIKAYIEELAKYKDAVKGEPYYPGTNVGNSY
                     GYSEKTGVLNTTDIESDKVTLNVPATAVYQEISKGNSDLNTQVDRLYNSMLAWEQIMD
                     LVYSERGVFKTQDLDGNGTVDDNEFNLTKNDMAPKSRMNIKYQRMFIGAFMYASGLHV
                     GVEYGSVPGLMNGVPFQIDDSGKATGGNLFGWGIGHEIGHVTDIGKMTYSETSNNVLA
                     LLAQTFDDKTHSRLEGSTMDKIYEHVTSNSLGIPSNVFERLGMLWQLHLAYDDDFTGS
                     MLKNNSDADLSNDTFYAKISRKYRALSSSDPINSLPKDQMLVAMASSVVEKDLRDYFK
                     AWGVEITPELNSIMDSKNYEKESRKIQYVNDEARRKILNENIASMAKDTKVSASFSDG
                     IQSGSLVKSNKISIDLDVDKDKDKILGYEIIRSDGNYLDGENGTQVKYRTVGFVDAKG
                     NSKTKFTDNISPLNNRAFTYKVVAYDYHLNPTEEFEVGTVKLSDEGKINKSAWSFITN
                     TVSDGDVRTENDSHGPLQNPEIDNIKDNDASTTYKGKIISKAEWNKDPQKDPDINVEE
                     NPYIIVDMKETLPIVGLKYTKPETEARKFSLKGLFNFRKNTSTTYNPLTNYKIQVSND
                     KKNWEDVSQGTFEYGQDLVGGGKDQDNEARVTFNKDGKLWTYQARYVKLISNNKSNIE
                     VAELDIIGPPGDNIEIGIINNADETRTNGIGKLAEDYVYQSDDSSTSEDETGKIPAGS
                     IVITGEYSGNPAFNLPLLIDKNNKTISGEALLFAEVPEKGELGEISKGTWVYYMPKEN
                     FDSLSDKVKAELYRYNDLQGDTPVGQRFVSDTMYVPVGAKNYDELKTISLTDSNSKAR
                     KATRNSIDISNKKVITVSNKVKGNIVRDK"
     regulatory      complement(8261..8346)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(8518..9921)
                     /locus_tag="KPD58_002444"
     CDS             complement(8518..9921)
                     /locus_tag="KPD58_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp9"
                     /protein_id="HBG0266205.1"
                     /translation="MKNSKKILAIGLTLFLVMVNTPMVNALTSVEQIKGNDRYETAAK
                     IADKQNYNTAILINSDNSLADGLSASGLAGALNAPILMTKQNQIPNTTMERLNKAKTV
                     YIIGSESTISKNVENQLLSKKKVVQRIFGENRFDTSIKIAEKIKEIKPIDKVIIANGF
                     TGEADAISASPVAARDGVPIILTDGNSVGFDTTGLKSYALGSSEIISDELVKSTNSIR
                     LGGTDRFETNKIVIQEFYKNSKEFYLSKGLQLTDALAASTIAKNAPVVLVENGSNKSI
                     LSGADKLTVLGGINQNVIKQCINQASPNQQGLYYNPNDRAFKERIKGKVYALTKQYRK
                     ENGVRALSVASRLEGLANDWSNLMANKKTLSHTINGKNSYSTFLKYLDWSEIKPGYIA
                     VQGENIIKYKIPDKPVYTNRDADDIGNFIFNEWKTNPEEGTNMLHKGYEIMGFGIAIT
                     GDKNLYATHEFYGRYKE"
BASE COUNT         2803 a         1883 c         1160 g         4353 t
ORIGIN      
        1 attgttaaga taatgaaaat aaaataagaa tgcatttaaa tatataactt tatatctaaa
       61 tacattctta ttttctattt acttaatatt tatttttcaa ttaaactctt aatcttgtct
      121 attacagatg aggctattcc tccacctact tcaaacaact ttgatgaatt tttacctttt
      181 aatgacgatt cttgtgattt atctaacgta tttgtagcaa gtataactgg tgaattgttt
      241 ttagctgcta atggtccaac tgctaaagca tctataagtt tatcttgatt tgcaactaca
      301 actccatcta gcttagattg agagtagaat ttatctatta ctttggcatt cgtctcttgt
      361 ctagtttgac ctgccaccct attgtttgat atatctttgt ttgctatttt tccaacttgt
      421 tcaatagcct tgttagatat tttttctact ccaccaataa aatatatatt tgacacctta
      481 tttgatttaa taaattgttc agcttcatta tctacaccat ctttttgtgt aagtactatt
      541 ggagtttttt ctttaccagc cagtgatgct attgataatg aatcagcttc tccatttcct
      601 gctcctatat acaacttgct tacattattc gtcttatcta tttcttttgc tatattaata
      661 gaagtttgat atcttgtatc tccacctatt ctttgtactg atatttttga atttacagct
      721 ttgatagctt caacaactga atttggcatt gagttgtttc cacctatcac aattactttt
      781 gatggattta gccttttcaa ttcttctaca gttttttgag gaagcttttc atttgatgat
      841 aaaagaattg gtgagttttt tacactagca agtggtgtag ctgtaagccc atccaccata
      901 cttttatctt ctccatttac tattacaact gtatctgatt ttgtccaacc actttgagat
      961 attttgatag ctgtctcaaa tctatcagca ccttttaatt ttttgactgt gacattagat
     1021 gtagaatcac ttgaacttcc tcctccagag cttccaccac tagatgagcc gcctgaatta
     1081 cttcctccac cattatttcc accattgcca ttatctggtg gtaattcatc cggttttgat
     1141 aattcatttt caactatgta ttctaaactt tttggaagtg taacttcaac atatttgttt
     1201 attgtagctt ttcttccctt agttgttttt tgtgctaatc caagttttaa tattacattt
     1261 gaaccacttt cttgtgtttc tttactaaca atagaatagt ttatcctaat attgtttaat
     1321 aaatattcaa gtgcttcact tttctctacc ttatactttt cagggtcatt tttactagaa
     1381 ccagttccac caattggttt tacttcactc tcattaataa gggtaaatat taatccttca
     1441 tcagagctta tattttcata atccactgtt ttgttgttat atgttacaat ttttaaagct
     1501 ggtttatcat ttgttccttt tcctaaaact ttatagtcag catttgagtt actgccagat
     1561 ttttttacag atattcttcc tatattcaat ctatttccgt ctagtaaatc ttcatttgat
     1621 gtaacatata tatctaatgt agttgtatta cttgatttgt tgtattctac tctacaagta
     1681 gcatttgcat ttccactttt tatctgagat tttatattat cagaccaagt aatcttttct
     1741 ttctgctcat tatctactac ttcaacctca ccttgtactt ttatactggc ttgaaatgaa
     1801 tatactatgt tttctactga gtccacagat atatctatag tatttacttt ctcatacgtt
     1861 tttgttcctt gtaaaactgg agctgcatat gcacatgtag cactaccagt tacagctact
     1921 gccatagttg atactgctat agttcttata aaagattttc ttttactcat taacacccgt
     1981 ccttatctat cattaactaa gtggataaat ttacttatcc acctagcttt tatatttaat
     2041 ttataaatta ccctaaattt atggttacat ttatatactt tacttttaat atctactatt
     2101 ttaaaactat tctagtacct aatcttatta gatatacaat gttagtattt ttacgttttt
     2161 agagttccac ctactattta tctctaacaa tattaccctt tactttattg cttacagtta
     2221 taaccttctt attactgata tcaatagaat ttcttgtagc ttttctagct ttactattac
     2281 tatcagtaag acttattgtc tttaactcat cataattctt agctcctaca ggtacataca
     2341 ttgtatcact tacaaatctt tgacctactg gagtatctcc ttgaaggtca ttgtatctat
     2401 atagttcagc cttaacctta tcacttaaag aatcaaagtt ttcttttggc atatagtaaa
     2461 cccatgtacc tttactgatt tcacctagtt cacctttttc tggaacttct gcaaataaaa
     2521 gtgcttctcc agatatagtc ttattgtttt tatctataag tagtggtaaa ttaaatgctg
     2581 gatttccact gtattcacct gtaataacta ttgaacccgc tggaatctta cctgtttcat
     2641 cttcacttgt gctagagtca tcactttgat atacataatc ttctgctaat tttccaattc
     2701 cattagtacg agtttcgtct gcattattta ttatgccaat ttctatatta tcacctggag
     2761 gtcctattat atctaattct gcaacttcta tattagactt attatttgag attagtttta
     2821 catatctagc ttggtatgtc caaagtttac catctttatt aaatgtaact ctagcttcat
     2881 tatcttggtc ttttcctcca ccaactaaat cttgtccata ttcaaaagtt ccttgactta
     2941 catcttccca gttcttttta tcattactta cttgtatctt gtaattagtt aaaggattgt
     3001 atgttgtgct agtattcttt ctaaagttaa ataatccttt taatgagaac tttctagctt
     3061 ctgtctctgg ttttgtatat tttagtccta ctataggtaa tgtttctttc atgtctacta
     3121 ttatatatgg attttcttct acatttatat cagggtcttt ttgagggtct ttgttccact
     3181 ctgctttact tattatcttt cctttatatg tagtacttgc atcattatct tttatattat
     3241 caatttcagg attttgtaat ggtccatgtg agtcattttc agttctaaca tcaccgtcag
     3301 aaacagtatt tgttatgaat gaccatgctg atttatttat tttcccttca tctgataatt
     3361 ttacagtacc aacttcaaat tcttctgttg gatttaggtg ataatcatat gcaactacct
     3421 tatatgtaaa tgctctgtta ttaagaggag atatattatc agtaaattta gtcttgctat
     3481 ttccttttgc atccacaaat ccaactgttc tgtattttac ctgtgttcca ttttcaccat
     3541 ctaaataatt tccatcactt cttattattt cgtatcctag tatcttatct ttatccttat
     3601 ctacatctag gtctatagat attttattag atttaactag agaaccactt tgtattccat
     3661 cagaaaaact agcacttact ttagtatctt ttgccatact agctatattt tcatttaaaa
     3721 tctttcttct agcttcatca tttacatatt gtatctttct tgattctttt tcgtagttct
     3781 tgctatccat tatactgttt aattctggtg taatttctac tccccatgcc ttaaagtaat
     3841 ctcttaaatc tttttcaaca acacttgatg ccattgctac tagcatttgg tcttttggta
     3901 aactattaat tgggtcacta cttgaaagtg ctctgtattt tcttgaaatc tttgcataga
     3961 atgtatcatt acttaaatca gcatctgagt tattctttaa cattgaaccc gtaaaatcat
     4021 catcataagc tagatgtaat tgccataaca ttccaagcct ttcaaataca ttgcttggta
     4081 tacctaagct atttgaagtt acatgctcat atatcttatc cattgttgaa ccttcaagtc
     4141 ttgaatgtgt cttatcatca aaggtctgag caagtaaagc aagtacattg ttactagttt
     4201 cgctatatgt catctttcca atatctgtaa catgtcctat ctcatgacct attccccatc
     4261 caaataagtt tcctcctgta gcttttccac tatcatctat ttggaaaggt actccattca
     4321 taagccctgg aactgaacca tattctactc ctacatgtaa tccagaagca tacataaatg
     4381 ctccaataaa cattctttgg tattttatgt tcatacgaga ttttggagcc atatcatttt
     4441 tagttaagtt aaattcatta tcatctacag ttccattacc atctaaatct tgtgttttaa
     4501 atactcctct ttctgagtaa actaaatcca ttatttgttc ccatgcaagc atactattat
     4561 ataatctatc tacttgtgta tttaaatcag aatttccttt tgatatctct tgatatactg
     4621 ctgttgcagg aacatttaat gttactttat cactttcaat atctgtagtg tttaacacac
     4681 cagttttttc tgaatatcca tatgaattac caacatttgt tcctggataa tatggttctc
     4741 cttttacagc atctttatat ttagctaatt cttctatata tgcttttatc tttttcttag
     4801 attcagaaac tcccgcagga ctcttatcat ttattgttcc atttagatta agatgtggta
     4861 tcttagtagc tcctgctaca cgtactttta tatctcctcc acttggagta tcttcagcgt
     4921 atcttatgta taaacttcct ccacattcta tatctaaact atgaagtttg cttaattgaa
     4981 ttacattttt tccagtgtta agatttattt ctccactctt atatgcacca gattctccat
     5041 agtactgtgt atatatcaac ttaggagttt ttccactttt acttccaaca tatactgtga
     5101 tttcatctcc tgctctagca gctactccta atgcctgcca gtcattttgc atacctaaat
     5161 tattggaatt tacagtatac tgtccgtttt tatctttata ttttcctaat ttatttattt
     5221 cttgatttac agtagttaca tcagtaagtg tggctctatc attatataag tcttgagcta
     5281 attttaattc tctctcaata actgtcttat atggatgata ttctccacta gctttatctg
     5341 gagtatttaa tctatctttt aattcagtta ttttttcttg agttacatct tcttttaatt
     5401 caacttgtag gccatccata aatagatttc ttacatcatc ttctatactg tcatactcat
     5461 aaaacttaag ttctgatata ttagacttat atgaatagga tgaacaaaca cttacttgta
     5521 tcttggtagc agttattggt tcttttaatc taaccattac atacttatta ttatttacag
     5581 ggtctgtctt tgtttcaaaa ggaacatccc ccagattgtg taattgacca ttttcatccc
     5641 aatatcgtac ctcaaagtca tttattgtct tatcagaaac atcaaaacga ggtgtagcca
     5701 taaaagtatc catagtataa gatttatcaa aagttattat tggtcctcta ttcgttccct
     5761 cagctgcatt atatgttggg aatgtccaat gtgttgtaaa gtccccatct actacattga
     5821 atttatcaaa tggattttta tcactatatt cacttttgtc atatccatct ggataaacaa
     5881 catctacaat gtgatttgtt ggtattccaa atccaccatt ttctttagga gtattaatta
     5941 atctatagtt aggtgttgtt ggtgcatcca tgctttcagt tgtacctata tatgttttag
     6001 acattccact agttcccaag tggttagtag ctgtcattct gacttcatat gttgtattat
     6061 ccttaagacc ttctatcttt gaacttgtcc cacgaataag cttatcattg tattttatcc
     6121 aagacccatt agagcctttt tctttatagt acaagtcaaa atctttagct tgtttatgat
     6181 ttttccagct tacatcaaga gatttaaatt tacctttaac atttatacct tctggtggtt
     6241 ctggttttgt gtctggaaca acttgaaatt ctaatatact atctgggcta aattgtatag
     6301 gtttatagtt tgaatctaca ttttctggta ctccgtctaa gttttctggt gttgcatctt
     6361 tataaccact agaccaatct ccatttaaag attgtatgct aactctatat agtccatatg
     6421 cttctatatc tctaacttct attgtatttt tactagtttg taatttttta gtagataata
     6481 cttttccctt actatcaatt ttgtctagta taagttcgta tgctgataca tttacttcat
     6541 tattccaata aaaagatact ttttcatttc caacagcagt gctagtcgat attttattta
     6601 ttttaggtat gttcatctct ggctttggta tttctttata tacattgttt aaaaattcaa
     6661 cttttgatat ttctgcaaga tttctatcgt ttgctgttgt agctgttatt tttatagtta
     6721 tcttttttac tgctacttgt ttaccaagat ttattactat atcactattt tgaacatctg
     6781 catatgtttc taatgctgac ctactatatg tagatgttgt agagttatca tctttactga
     6841 tattaccctc ttcttttgga gtatcttcat ctgaattagg tttatccatt gatgaatttt
     6901 ctttatcatt atttttatct tgattggtat tagagtttgc ttcttgttta tcgctatttt
     6961 cctgttgttt atcattattt tcatctttat tatttgagtt attatcagtc tggtttatat
     7021 tttcatcttt attatttact tcttcttgat ttacttcttc ttgttcgtta tttacttcct
     7081 cttgttgctc ctcatctttt aagtttttca tatattcttt gttttcataa ggaatctgtt
     7141 gaacattacc atctttgtca gtaacctcaa ctattccttt tgctggagca gtaccttctg
     7201 gagcttttat attgatgact tccatattaa gtgcttctga taaagctata tccattactg
     7261 ctggtttttc ctcagatatt tcatcatttt ttgaagatat tgtaactcca ttattttctc
     7321 cactaaataa attttctaca ttaccattaa tatctacatc actatttcct tttaattcaa
     7381 ccttacttgt atctactata ggattggtag atactatact ttctaggctt tttgcatctt
     7441 cactttgtcc taaatttttc tgtacataat ctaagtcaac aatatctacc tttccatctc
     7501 tatttaaatc aaattctctt ttatttgaat ctatattctc taaaacctta ttataatcac
     7561 tcttatctac tttaccgtct ccatttacat cacccattaa gaacaattca taatttaaag
     7621 gtttattttt attatctaca cttgctctat tgcttaataa gactctttgt gaataacttg
     7681 atatatcaat gtctttaatt tctttgttct taaatccatc tccagagact tctacattgt
     7741 attttccaac aggtaaatta ttaaatacaa tattataata tgatatattc tcatctttat
     7801 tttttatttc acttctactt gcagataatt tttttatagt atattctata ttatctgtcc
     7861 caacatttac tgttcctgaa tctttttctc caagctttat tattccacta ttgttactat
     7921 catcttttaa tcgtaactgc atattagtca tactttctga ctcgctattt tttattggca
     7981 gtgaaaaatt taaatctact tcaagactcc cccttaactt ttctgtaact gatttttcaa
     8041 ctgcccttag cttcccctct aatataggtt ctgcaaaaac gtttagtggt gcactacaca
     8101 tagatactgc tagtacacaa gatattgcct ttttcatgaa ataacaatcc tcccttaata
     8161 aatatctaat ttttcatgaa tttaaatact ataagtttca gacaataaaa aaagacactc
     8221 aaagagaatc ttaattataa aataaaaaga ctttactctt tttagcaact ggctgatatg
     8281 gctttaaaag ctttaatccc tatagttttg cgtcattaga tttccctaat tttgccattt
     8341 aattttcact actttcttac ccattttata ctatatttaa actttttaat ataatttttt
     8401 tagattaaat tataaaaaaa taacatttat attaatttat taatataaat gctattttcc
     8461 attataattt aattatttat atagcttcct tacaagcata attttgtgta tattttctta
     8521 ttctttatat cttccataaa attcatgtgt agcatataga ttcttatctc ctgttatagc
     8581 tataccaaat cccattattt catacccttt atgcaacata ttagttcctt cttctggatt
     8641 tgtcttccac tcattaaaaa taaagtttcc tatatcatct gcatctctat ttgtatatac
     8701 aggtttatct ggaattttat atttaattat attttcacct tgaactgcta tatatccagg
     8761 tttaatctca ctccaatcta agtatttcaa aaaagtagaa tatgaatttt ttccatttat
     8821 cgtatgagat aaagtcttct tatttgccat taaattagac caatcatttg ctagaccctc
     8881 taatctgctt gctacagata acgctcttac tccattctct tttctatact gctttgtaag
     8941 agcatatact ttacccttta ttctctcttt aaatgctcta tcatttggat tgtaatataa
     9001 accttgttga tttggtgaag cttgatttat acattgcttg ataacattct gatttatgcc
     9061 tcctagtaca gtcaatttgt ctgcaccact taaaatactc ttgttactgc cattttcaac
     9121 taaaactaca ggagcatttt ttgctattgt agaggcagcc aaagcgtctg ttaattgtaa
     9181 gcctttactt agataaaatt ctttactatt tttataaaac tcttgtataa ctatcttatt
     9241 agtttcaaat ctatctgtgc cacccaatct aatagaattt gtgctcttaa ctaattcgtc
     9301 gcttattatt tcactagaac caagcgcata actctttaag cctgtcgtgt caaatcccac
     9361 actatttcca tctgtaagta ttataggtac tccatctcta gcagctactg gtgatgcact
     9421 tatcgcatct gcttctcctg taaatccatt ggctataatt actttatcta ttggcttaat
     9481 ttctttgatt ttttcagcta tttttatact tgtatcaaat ctattttcac caaatattct
     9541 ttgtacaacc ttctttttgg atagtaactg attctcaaca tttttactta ttgttgattc
     9601 tgaacctatt atatacacag tctttgcttt gtttaatctt tccatagttg tgtttggaat
     9661 ctggttttgt tttgtcatca aaataggcgc atttaaggct ccagctaaac cacttgcact
     9721 tagaccatct gctaagctat tatctgagtt gattagtatt gctgtattgt agttttgctt
     9781 atctgctatt tttgctgctg tctcatatct gtcattccct tttatttgct caactgatgt
     9841 caacgcattt accataggag tatttaccat tactaaaaat agtgtaagtc ctatagctaa
     9901 tatcttttta ctattcttca tattattttc tacccccaat gtacttttca tattttttat
     9961 tagtactcat acttattttt aatcaaaagt tacaattttg ttaccatatt ttatatatga
    10021 taccatagtt atccatacaa ctcaaacaat atacctaaag tttttattct ctataaattc
    10081 aaaacattat aatttatttt atttaattac gttataagtt caaaataatt tatgttagta
    10141 atatgtttaa tcattataag aagtatcttt ttataataat ttttgcaata tgaatttct
//
LOCUS       DAELLV010000130        10151 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.130, whole genome shotgun sequence.
ACCESSION   DAELLV010000130 DAELLV010000000
VERSION     DAELLV010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10151
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.130"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            340..1815
                     /locus_tag="KPD58_002445"
     CDS             340..1815
                     /locus_tag="KPD58_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBG0266206.1"
                     /translation="MDSEFANAVELSYECAPLLESLKEYSEKDIACFDVPGHVKNRGV
                     AILNKYLGESLMKMDINSSPTMDNVSAPNGIIKNAQDLLAQAYMADEAFFITNGTTQA
                     IHAMILSVIKPGEKVLLPRNIHKSVINALILCGGIPIFIQPEFDEKLGISLNITLEKV
                     RTEIEKDCNIKALFFLNPTYYGVCADLESIIELCHKNNVLVLVDEAHGAHFPFHLDLP
                     PSAISLGADMVAVSIHKTGGALTQSSALLLNRDNVSFEKVLQSINMLQSTSASYLLMA
                     SIDGARVNLVENGEKQLSKALNLSRYAKSKLNKIDGIKVLSTEILKQKGVKFIDETKL
                     CINVKELNLTGFEVYDLLYKNFSIQVELGDSYNILALVSIGTNKSDIDRLVKALSIIA
                     KIYRKESTLNEFNMVQINPIIKLNPREAFYAPKESVEINSCIDRICGESIMAYPPGIP
                     IIAPGELITEEIMEYIIFLKNSNAYLTDVQDKNLDRILVIK"
     gene            1902..2315
                     /gene="speD"
                     /locus_tag="KPD58_002446"
     CDS             1902..2315
                     /gene="speD"
                     /locus_tag="KPD58_002446"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HBG0266207.1"
                     /translation="MKFETLGRHILVEYYNCDENVLKDPRLIENFMNDSALNAKATIV
                     DSVFHHFNPWGVSGAVIIAESHLTIHTWPEYGYAAADFFTCGDIDPWKSFELLEQLLK
                     SERSESTEIPRGLTTKIKKYSKKDLGKITHKPEAV"
     gene            2345..3196
                     /gene="speE"
                     /locus_tag="KPD58_002447"
     CDS             2345..3196
                     /gene="speE"
                     /locus_tag="KPD58_002447"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HBG0266208.1"
                     /translation="MELWYTEEWTENVRFSIKVNKHLFEGKSQFQRIDVFDSDEFGKF
                     LTIDGLMMVTYKDEFIYHEMITHVPMATNLNIKKVLVIGGGDGGTVRELSCYPQIEKI
                     DMVEIDKMVVDVSKEYMDICSCKLDDKRVSLYFEDGVNFVKCAHDKSYDLIIVDSTDP
                     IGPGEGLFSTDFYKDCYRILTDDGILVNQSESPYFDFNAKEMKRANKKLKQIFPISEV
                     YQAHIPTYPSGHWLFGFASKKLNPVKNQDRNGWEKLSLKTKYYNSDIHLGSFMLPQYV
                     KEMLDEE"
     gene            3186..4064
                     /gene="speB"
                     /locus_tag="KPD58_002448"
     CDS             3186..4064
                     /gene="speB"
                     /locus_tag="KPD58_002448"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HBG0266209.1"
                     /translation="MKNNFYHRNTFMSMNKNYEESNLIVFGVGFDGTTSNRPGARFAS
                     SSMRKEFYGLETYSPFLDLDLEDYNICDYGDLEISVGSTEQVLKEIYQETYKIVRDSK
                     VPFMIGGEHLVTLPAFKAVHEKYNDIYVIHFDAHTDLREEYNNSKNSHATVIKRIWDI
                     VGDNKIFQFGIRSGTKEEFKFATEEKHTYMEIGGIDTFENIVNMLNGKNIYLTIDLDV
                     LDAAVFPGTGTPEPGGVNYREFQEIFKIIKNSNINIVGCDIVELSPDYDTTGVSTVIA
                     CKILRELCLIISDKIK"
     gene            complement(4244..4444)
                     /locus_tag="KPD58_002449"
     CDS             complement(4244..4444)
                     /locus_tag="KPD58_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBG0266210.1"
                     /translation="MKNGIVKWFNNEKGFGFISVEGEDDVFVHFSAIQNDGYKTLEEG
                     EKVSFDITQGNRGPQAENVNRI"
     gene            4911..6401
                     /locus_tag="KPD58_002450"
     CDS             4911..6401
                     /locus_tag="KPD58_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG0266211.1"
                     /translation="MFQFETQIHKLRHDVLTAIARLGKEDNLTREAMDNIQYEIIKGE
                     KPTYRCCVYKERAIISERVNVSMGLQPGQDKIDLDTMDIEEDEQIIYILEAACDSCPI
                     NEFTVTDICRGCLAHRCKEACKFGAITHVGGMAYINHELCKACGMCKKACQYDAISEV
                     VRPCKSVCPTNALGFDRENMKAMIHEDKCLNCGACMSACPFGAISDKSLIAPVARKLV
                     QKEKMYAVVAPAITGQVEANVTYGQIKNAIKSLGFVDVLEAACGADAVTVHESLEFAE
                     RMEKGEAFMTNSCCPGFVGYIEKTYPNHVGKISSTVSPMIASGRFIKSHEPDAKVVFI
                     GPCTAKKSEAAKKELKGAVDYVLTFEEIMALFEAFDIDLAKCSNEDIDDASIFGRGFG
                     AAGGLTKAIENYLAEKGIGVNFEPVKISGSREIKKTMTMANLGKLEGNFIEGMMCEGG
                     CINGSGKFLSGAKVKATFDRKNSQSTKKSVLANTKIKDFSNINLEK"
     gene            6681..8177
                     /locus_tag="KPD58_002451"
     CDS             6681..8177
                     /locus_tag="KPD58_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG0266212.1"
                     /translation="MFEFETQLKRLKHEVLTAVARLSIEENLTKENIEKIPYEIIKGE
                     KARYRCCVYKEREVVLERAQLALSLTPNSKYGDINPDSVSLDTDEQIIYIVEAACDRC
                     PINEFTVTELCRGCLAHRCKEACKFGAISYINGRAYINHDLCKACGMCKSSCQYDAIS
                     EVVRPCKSVCPTGALDFNRNTMKAMIHEDNCINCGACMSACPFGAISDKSLIAPVAKK
                     LAKKENMYAIVAPAITGQIDSNITYGQLKNAIKSLGFVDMIEASCGADAVTVHESNEL
                     IERIKSGDNYMTNSCCPGFFKYIEKMFPDEKDRISSTVSPMVATGRLVKKIDSDAKVV
                     FIGPCTAKKSEATEKSLKDSVDYVLTFEELMALFEAFNIDLSKCSNEDINDGSIFGRG
                     FGASGGLAKSVQNYIAEKGLSFDFEPIKVSGPRQIKKTMTMAKLGRLEGNFIEGMMCE
                     EGCINGAGKFLSGVKAKNTFDRKNGQSTKKSVLSNDKIKEFEDVNLER"
     gene            8322..9248
                     /locus_tag="KPD58_002452"
     CDS             8322..9248
                     /locus_tag="KPD58_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0266213.1"
                     /translation="MRKIVLKDILFLVTTLSIIVILLLVTNITTNSKIKSTSESYLKS
                     DKNSVRQNGIQRSTKSSTKNTAFKTTILNTGKSDCILVEIGNKVIMIDTGEDKNGKQI
                     VDRLKEKGINTLDYLILTHLDKDHIGGVDSVLSSVKVKNIIQANYKKDSKQYDEYIDS
                     LKKADIEPVLLKDNMNIVINGAEINIHPASKSKYESSNDYSIITNISYGAHKFLFAGD
                     AEEERLSEFINGNTLKYDFVKMPHHGRYDKLTETFLESISPQYAVITCSEKKEPEEGV
                     LKILERLNIKTFLTSNGEVVINSDSKTLSVNQ"
     gene            9366..9557
                     /locus_tag="KPD58_002453"
     CDS             9366..9557
                     /locus_tag="KPD58_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266214.1"
                     /translation="MLDRFFGEGGFGGCAWWIIILFFLFLAFQDCWAEMDLCAWIPFL
                     ILLLILCECGDVFDGGCGC"
     gene            9780..10130
                     /locus_tag="KPD58_002454"
     CDS             9780..10130
                     /locus_tag="KPD58_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266215.1"
                     /translation="MAIVDKIFNEIKLMVARLLLGKKYSEYEEKDLVYNTEEEVILIT
                     LKRLVFQGNVNEAEEILFDKAKSVNSENMQYIAIEFYTMLMEKTDEELEAMNFSKQEV
                     YQGIEDIRKVLNLE"
BASE COUNT         4084 a         1054 c         1815 g         3198 t
ORIGIN      
        1 aaagtttagt aataaaatac ttataaatta aaaatagtaa actcaaaagc ttactaaaac
       61 tcaaaaaagt aaaaaaacca aaataataaa aaataagaaa gtttataaat actaagtttt
      121 aaaaaaatta atataaacaa caaaaaatta aaaagtttat aaatattgat atagctaatg
      181 tatatgtttt tgagtaactt caaaaaaaca cttgtaaaaa tgctgagatg ttaatataat
      241 aaatatcatg agtttaataa aagtaaaaat aaaaaataga aataataaat aataaatatt
      301 aaaaatattt gaatatagag aggagatgaa tttgaaatca tggatagcga gtttgcaaat
      361 gcagttgaat taagctatga gtgtgctcca ttattagagt cgttaaagga atactcagag
      421 aaagatatag cttgttttga tgtgcctgga catgtaaaaa atagaggtgt agctatatta
      481 aataaatatc taggtgaaag tctcatgaaa atggatataa attcatcacc aactatggat
      541 aatgtatctg cgccaaatgg gattataaaa aatgcacaag atttgctagc acaagcatat
      601 atggctgatg aggcattctt tataacgaat ggcacaactc aagcaataca tgcaatgatt
      661 ttgagtgtta taaaaccagg tgaaaaagta ttacttccaa gaaatataca taaatcagta
      721 ataaatgcgt taatactttg tggaggaata ccaatattta tacaaccaga atttgatgaa
      781 aaactaggta taagtttaaa tattactctt gaaaaagtta gaacagagat agaaaaagat
      841 tgtaatataa aagcattatt tttccttaat ccaacttact atggagtttg tgcagacctt
      901 gagagtatta tagaactatg ccataaaaat aatgttttag tgttagttga tgaagctcat
      961 ggtgcacatt ttccatttca tttagattta cctccttctg caataagttt gggagctgat
     1021 atggtagctg taagtataca taaaactgga ggagcattga cacaatcttc agcactctta
     1081 ttgaatagag ataatgtaag ttttgaaaag gttcttcaat caatcaatat gctacaatca
     1141 acatcagcat cttatttgct tatggcaagt atagatggtg ctagagttaa tctagttgag
     1201 aatggtgaaa agcaactgtc aaaagcatta aatctatcta ggtatgcaaa atctaaatta
     1261 aacaaaattg atggaataaa agttttatct acagaaatat tgaaacaaaa aggtgttaaa
     1321 tttatagatg aaacaaaatt gtgtataaat gtaaaagaat taaatttgac tggatttgaa
     1381 gtatacgatt tattatataa aaatttttca attcaagtag aattgggaga ttcatataat
     1441 atactggcac ttgtgtccat tggaacaaat aaatcagata tagatagatt agtaaaagca
     1501 ctttctataa ttgcaaagat ttatagaaaa gaatcaacat taaatgaatt taatatggtt
     1561 caaataaatc caattattaa acttaatcca agagaagctt tttatgcacc aaaggaaagt
     1621 gtagaaatca attcatgtat agatagaatt tgtggagaat caataatggc atatccacca
     1681 ggtataccga ttattgctcc aggagaattg ataacagaag aaattatgga gtatattatt
     1741 ttcttaaaaa atagcaatgc atatcttaca gatgtacaag ataaaaactt agatagaata
     1801 ttagtaataa aataaattac tttattttgc aagggcaagt atttgttaag tagagtttat
     1861 caaatagaac ttattaaaag tataaaggag ttggggttga aatgaaattt gaaacactag
     1921 gaagacatat actagtagaa tactataatt gtgatgaaaa cgtacttaaa gacccacgtc
     1981 ttatagaaaa ttttatgaat gattcagcat taaatgcaaa agcgacaatt gtggattctg
     2041 tattccacca ttttaatcct tggggggttt caggagctgt tataattgct gaatctcatt
     2101 tgacaataca tacatggcct gaatatggtt atgcagcagc ggattttttt acttgtggtg
     2161 atatagaccc atggaaaagt tttgaattat tagagcaact tttaaagtct gaaagaagcg
     2221 agtctacaga aattccaaga ggcttaacta caaaaattaa aaaatattca aaaaaagatt
     2281 taggtaagat tactcataaa ccagaagcag tataaaacat acaagagaaa gtgaggatta
     2341 ttatatggaa ctttggtata cagaagaatg gacagaaaat gtaagatttt ctataaaagt
     2401 aaataaacat ctatttgaag gaaagtctca atttcaaagg atagatgtat ttgatagtga
     2461 tgaatttgga aaatttctaa caatagatgg attaatgatg gtaacatata aagatgaatt
     2521 tatataccat gagatgataa ctcatgttcc tatggctaca aatttaaaca taaagaaagt
     2581 attagtcata ggtggaggtg atggaggtac agtaagagaa ttaagctgtt atcctcaaat
     2641 agaaaagatt gacatggttg aaatagataa aatggttgta gatgtatcta aggagtatat
     2701 ggatatatgc tcatgtaaat tagatgataa aagagtgagt ttatattttg aagatggtgt
     2761 taattttgtt aagtgtgcac atgataaatc atatgacctg ataatagtag attcaactga
     2821 cccaatagga ccaggcgaag ggcttttctc aactgatttt tataaggatt gttatagaat
     2881 actgactgat gatggaattt tagtaaatca aagtgaaagt ccgtattttg actttaatgc
     2941 taaagagatg aaaagagcta ataaaaagtt aaaacagata tttccaatat cagaagttta
     3001 tcaagcacat ataccaacat atccatctgg tcactggttg tttggatttg cttcaaaaaa
     3061 acttaatcca gtaaaaaatc aagatagaaa tggatgggag aaattaagtt taaaaactaa
     3121 atattataat agtgatatac atttaggttc gtttatgtta cctcaatacg tgaaggagat
     3181 gctagatgaa gaataatttc tatcatagga acacttttat gagtatgaac aaaaattatg
     3241 aagaatctaa tcttatagta tttggtgttg gatttgatgg aactacttct aacagacctg
     3301 gggctagatt tgcaagtagc tctatgagaa aagaatttta cggtcttgag acatatagtc
     3361 cttttttaga tttggattta gaggattata atatatgtga ttatggagat ttagaaatta
     3421 gtgttggaag tacagaacaa gtcttaaaag aaatctatca agagacatat aagattgtta
     3481 gagattcaaa ggtacccttt atgattggag gagagcattt agttacatta ccagccttta
     3541 aagcagtaca tgaaaagtac aatgatatat atgtaattca ttttgatgcc catactgact
     3601 tgagggaaga atataataat agtaaaaatt ctcatgcaac agtaattaaa agaatatggg
     3661 atattgtagg tgataataaa atatttcaat ttggtataag gtctgggaca aaagaagaat
     3721 ttaaatttgc tacagaagaa aaacacacat acatggaaat aggaggaata gatacgtttg
     3781 aaaatatagt taatatgcta aatggaaaga atatttatct aactatagat ttagatgtat
     3841 tggatgcagc tgtgtttcca ggaacaggta caccagaacc tggtggtgta aattatagag
     3901 agtttcaaga gatttttaag attataaaaa actctaatat aaatatagtt ggttgtgaca
     3961 ttgtagagtt aagccctgat tacgatacaa caggtgtatc gacagttata gcttgtaaaa
     4021 tcttaagaga gttatgctta ataatatctg ataaaattaa atagagtatt ttatataaat
     4081 agtatatatt ataatataag aagttatttt aaaaaagtta gtttaagttt gacgcttttc
     4141 taaagtaact tcttattttt gtaattaaaa ataaatataa taaacaacaa aaaagagccc
     4201 tcgatagctc cttttctaaa atatatatat ttaaattaaa taattatatt ctatttacgt
     4261 tttcagcttg aggtcctcta ttaccttgag ttatatcaaa gctaaccttt tcgccttctt
     4321 ctaaagtttt gtatccatca ttttgtatag ctgagaaatg tacgaaaaca tcatcttctc
     4381 cctctacaga tatgaaccca aatccttttt cattgttaaa ccattttact attccgtttt
     4441 tcattataag aaaacctccg cattattaaa aaatacaaac taaaaaaata agtctgttct
     4501 attaatataa cataaatttt aaataaaagc aaataaaatt tttaaaaaaa ttaattactt
     4561 ttagaattta ataaaaattg ttaaattata cagtgataag tattgtaaaa tttgacgaaa
     4621 atgtaaatgt agataataaa tttaatatca aaaaatgtaa caaaaaggta tgtaagtaaa
     4681 aagtaaaatt aaaatatata ataaaaaata ttatatatta gattgaaaag taaaaataaa
     4741 aatagtgaaa tttcagaaat atcaataata ttatataata aaacaaaaaa acactatata
     4801 tattcgataa aataaaattt aaacatataa tattttatgt atatattaat aaaaggacat
     4861 attgtgttat aatggtatcg tataaaatca tttacggggg tggtattaaa atgtttcaat
     4921 ttgaaacaca aatacacaaa ttaagacatg atgttttaac tgcaatagct agacttggaa
     4981 aagaagataa ccttaccaga gaagcaatgg ataatattca gtatgaaata ataaaaggtg
     5041 agaaacctac atacagatgc tgtgtttata aagaaagagc tataatatct gagagagtaa
     5101 atgtaagtat ggggttacaa ccaggtcaag ataagataga tttagataca atggatatag
     5161 aagaagatga acaaattata tatattttgg aagctgcatg tgacagttgt ccaataaatg
     5221 aatttacagt aacagatatt tgtagaggat gtctagcaca tagatgtaaa gaagcttgta
     5281 aatttggagc tataacacat gtcggaggaa tggcctatat aaatcatgaa ttatgtaagg
     5341 cttgtggaat gtgtaaaaaa gcttgtcaat atgatgccat ctcagaggtt gttagaccat
     5401 gtaagagtgt ttgtccaact aatgccttag gatttgatag agaaaatatg aaagctatga
     5461 tacatgagga taaatgttta aactgtggag catgtatgtc agcatgtcca tttggagcaa
     5521 tatctgataa gagtttgata gctcctgtag ctagaaagtt agttcaaaaa gagaaaatgt
     5581 atgcggtggt agctccagct ataacaggtc aagttgaagc aaatgttaca tatggtcaaa
     5641 taaaaaatgc tataaaatca ctaggatttg ttgatgtatt agaagcagct tgtggagcag
     5701 atgcagttac agttcatgaa agcttagaat ttgctgaaag aatggaaaag ggagaagctt
     5761 ttatgacaaa ttcttgttgt ccaggatttg ttggttatat agaaaaaaca tacccaaatc
     5821 atgtagggaa aatatcttct acagtatctc ctatgatagc tagtggtaga tttataaaaa
     5881 gtcatgaacc agatgctaaa gttgtattca taggaccatg tacagccaaa aagagtgagg
     5941 cagctaagaa agaattaaaa ggagctgttg attatgtact tacttttgaa gaaataatgg
     6001 cattatttga ggcatttgat atagacttgg ctaagtgttc aaatgaagat atagatgatg
     6061 catcaatttt tggaagaggt tttggagctg ctggtggtct tacaaaagct atagaaaatt
     6121 atcttgctga aaaaggtatt ggtgttaatt ttgaacctgt aaagatttct ggttcaagag
     6181 aaatcaagaa aactatgact atggctaatt taggaaagct agaaggtaat tttatagaag
     6241 gaatgatgtg tgaaggtggt tgtataaatg gttctggtaa attcttatca ggtgcaaagg
     6301 ttaaagccac ttttgataga aaaaattctc aatcaactaa gaaaagtgtc ttagcaaata
     6361 caaagataaa agattttagt aatataaatt tagaaaaata aagataatac aaagcaacaa
     6421 aaatacatat tgttaataga gttgttctaa gttgaggttt aacttttgga caactctatt
     6481 tttatttata tatttaatta ttgaacaact aaaataaaaa ttatatacac ttaaaataaa
     6541 aattacatat ataatatttt gtatataact taacaaagta tatgcctatg ttataataat
     6601 ttatagaaat aaataaaaaa tacaatatac ataatagtag aaatatagca aaaaggtgtg
     6661 atttataggg aggattactt atgtttgaat ttgaaacgca actaaaaagg ttaaaacatg
     6721 aagttttaac agcagtagca agactatcta ttgaagaaaa tttaacaaaa gaaaatatag
     6781 aaaaaattcc atatgaaata attaaaggag aaaaggcgag atatagatgt tgtgtttata
     6841 aagagagaga agtagttctt gaaagggcgc aacttgcatt aagtttaact ccaaatagta
     6901 aatatggaga tataaatcca gattcagtta gtttggacac agatgagcaa attatatata
     6961 tagttgaagc agcatgtgat agatgtccaa taaatgaatt tacagttaca gagctttgta
     7021 gagggtgttt ggctcataga tgtaaggaag catgtaaatt tggggcaatt tcttatataa
     7081 atgggagagc atatataaat catgatttat gtaaggcttg tggaatgtgc aaaagttcat
     7141 gtcaatatga tgctatatca gaagttgtaa gaccttgtaa aagcgtttgt ccaacagggg
     7201 ctttggattt taatagaaac actatgaaag caatgataca tgaagataat tgtataaatt
     7261 gtggagcatg tatgtcagca tgtccttttg gagcaatatc tgataagagt ttgatagcac
     7321 ctgttgcaaa aaaacttgca aagaaagaaa atatgtatgc aatagtagct ccagctataa
     7381 caggtcaaat agatagtaat attacatatg gtcagttaaa aaatgctata aaatcactgg
     7441 gatttgtaga tatgatagaa gcttcttgtg gagcagatgc agttacagtt catgaaagca
     7501 atgaacttat tgaaagaata aaatcaggag ataattacat gacaaattca tgttgtccgg
     7561 gattttttaa gtatatagag aagatgttcc cagatgaaaa agatagaata tcatctacag
     7621 tgtctcctat ggtagcaaca ggaagattag taaaaaaaat agattctgat gctaaagttg
     7681 tatttatagg tccatgtaca gctaaaaaga gtgaggcaac agagaaaagt ttaaaagatt
     7741 cagtagatta tgtacttact tttgaagaac taatggcatt atttgaagca tttaatatag
     7801 atttatcaaa atgttcaaat gaagatataa atgatggttc gatttttgga aggggatttg
     7861 gagcatctgg tggacttgcc aaatcagtac agaactatat tgctgaaaag gggcttagtt
     7921 ttgattttga acctataaaa gtatctggtc caagacaaat taaaaagaca atgacaatgg
     7981 ctaaattggg aagattagaa ggtaatttta tagaaggaat gatgtgtgaa gaaggttgta
     8041 ttaatggagc aggtaagttt ttatcaggtg taaaagctaa aaatactttt gatagaaaaa
     8101 atggacaatc tactaaaaag agtgttttat ctaatgataa gattaaagaa tttgaagatg
     8161 taaatttaga aagataattg ttttggtaca gtcatttttt tatcatgttt ttatcatgcc
     8221 acaaatatgc ctaaaatttg ccctttttat atgtgtattt tacagagtta aggttgctat
     8281 actataaata tgtaataaat aaaatttata ggagcttttg gatgaggaaa atagtattaa
     8341 aagatatcct gtttttagtt actacattat ctataatagt aatattattg cttgtaacaa
     8401 atataactac taatagtaag ataaaatcaa caagtgaatc ttatttaaaa agtgacaaaa
     8461 atagtgtgag gcaaaatggt atccagagga gcacaaagag tagcacaaaa aatactgcat
     8521 ttaagactac tatactaaac actggaaagt ctgattgcat attggttgag ataggtaaca
     8581 aagtcataat gattgataca ggagaagata aaaatggaaa acaaatagtg gataggttaa
     8641 aagaaaaagg aataaataca cttgactatt taattttaac tcatttagat aaggaccata
     8701 taggtggagt agatagtgtg ttatctagtg ttaaagtaaa gaatattata caagcaaatt
     8761 ataagaaaga tagtaagcaa tatgatgaat atattgactc acttaaaaaa gctgatatag
     8821 aaccagtgct actaaaagat aatatgaata ttgtaatcaa tggtgcagaa ataaacatac
     8881 atccggcttc aaaaagcaag tacgagagtt caaatgatta ttcaataatc actaatataa
     8941 gctatggagc acataagttt ttatttgcag gagatgcaga ggaagaaaga ttgtcagagt
     9001 ttataaatgg aaatacttta aaatatgatt ttgttaaaat gcctcatcat ggaagatatg
     9061 acaaattaac tgaaacattt ttagagtcta tttcacccca atatgctgtt attacatgtt
     9121 cagagaaaaa agaaccagag gaaggtgttt taaaaatatt ggaaagattg aatataaaaa
     9181 catttttgac ttctaatgga gaggtagtta tcaatagtga tagtaagaca ttgagtgtga
     9241 atcaataatt tttttaaaaa tatctaaaaa aatttaaaaa aatcattagc aaatattacc
     9301 ccttaaacat agtatatttt aggagctgaa actccacata caaaatgact aggaggtaac
     9361 atattatgtt ggatagattt tttggtgaag gcggatttgg tggatgtgct tggtggataa
     9421 taatattatt cttcttattc ttagcattcc aagattgttg ggctgaaatg gacttatgtg
     9481 catggatacc tttcttaata ttattattaa tactttgtga atgtggtgac gtttttgatg
     9541 gaggatgtgg atgctaaaaa aatcaattta gatgatttaa taaagcttat ggtgggtata
     9601 aataaaatac caaaccaatc acaatctagt gtttgaaggt agtcggaaat cccccgctac
     9661 cttctttttt ttattgttaa aatttatttt tgaattattt catttacata taataatttt
     9721 tacgataaag tctgatataa ttaaaaagaa aataaaattt atttatagga gatgtaaaaa
     9781 tggctatagt ggataagatt ttcaatgaga taaaacttat ggttgcaaga cttttattgg
     9841 gaaaaaaata cagtgaatat gaagaaaaag atttagtgta taatacagaa gaagaagtta
     9901 tacttataac tttaaaaaga ttagtttttc agggaaatgt aaacgaagca gaagaaattt
     9961 tatttgataa agctaagagt gtaaatagtg aaaatatgca gtatatagca atagaatttt
    10021 atactatgct tatggaaaaa actgatgaag aattagaagc tatgaatttt tctaaacaag
    10081 aagtttatca aggtatagaa gatattagaa aagtattgaa tttagaataa aagatatatg
    10141 aaaatattga a
//
LOCUS       DAELLV010000131        10043 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.131, whole genome shotgun sequence.
ACCESSION   DAELLV010000131 DAELLV010000000
VERSION     DAELLV010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10043
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.131"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(46..2184)
                     /gene="vanT"
                     /locus_tag="KPD58_002455"
     CDS             complement(46..2184)
                     /gene="vanT"
                     /locus_tag="KPD58_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HBG0266216.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKHLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVNMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINDCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLAE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            complement(2202..3008)
                     /locus_tag="KPD58_002456"
     CDS             complement(2202..3008)
                     /locus_tag="KPD58_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG0266217.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            complement(3005..4105)
                     /gene="vanG"
                     /locus_tag="KPD58_002457"
     CDS             complement(3005..4105)
                     /gene="vanG"
                     /locus_tag="KPD58_002457"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HBG0266218.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            complement(4366..5448)
                     /locus_tag="KPD58_002458"
     CDS             complement(4366..5448)
                     /locus_tag="KPD58_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG0266219.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFMNHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIVGNL"
     gene            complement(5498..6199)
                     /locus_tag="KPD58_002459"
     CDS             complement(5498..6199)
                     /locus_tag="KPD58_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanG-Cd-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBG0266220.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            complement(6633..9164)
                     /locus_tag="KPD58_002460"
     CDS             complement(6633..9164)
                     /locus_tag="KPD58_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG0266221.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLHSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            complement(9272..9889)
                     /locus_tag="KPD58_002461"
     CDS             complement(9272..9889)
                     /locus_tag="KPD58_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBG0266222.1"
                     /translation="MCKKTYLLVVSILIAGLLTACSGSPTTDSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIMTPQQAMEIALKEQNG
                     IVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
BASE COUNT         3346 a         1818 c         1053 g         3826 t
ORIGIN      
        1 gtaaaataaa ttacacagta taacaaatat tactatttaa ttgagttact tctttataac
       61 atttaacctt cttcctactc tgctcaataa ttcatttgat atactacatg agttatttgc
      121 aacttcagaa gcagataatt cagctaaatt atctcttcct attattattg ccgtattacc
      181 aacttctaca ttaggtatat ctgtaatatc aacagcaagt tggtccatgc aaattcgacc
      241 cactattgct gctctacaat catttattat tacgtaactt tttccacagg ataaatttct
      301 aggtatacca tcggcatagc caatagataa tatagcaatt acgctatctc tgcttgcaat
      361 aaagactctt ccatatccaa aactatcatc tttttgtatt tttctaataa gaattacttg
      421 tgattttaag gataaaacag gtcgtaaatc aagttgcaat ttagtcgtat catttggaga
      481 acttaatatc ccgtataatg caattccaat tcttacataa ttacacttaa gattgggata
      541 attaaaaaaa ccataactac tttgaatatg tactttagga atttttatac ctttttttaa
      601 cagaaaatca attaacttat aaaaacattc aatttgttta tttgtaaagt ctacatcttc
      661 atctttcaga ctatctgaaa cacacaagtg tgtaaatatg ccacatatat ctaagtattt
      721 taatttaaat gcctttaaaa ttgacctcac atcattaaca tcaaaaccca gtctgtgcat
      781 acctgtatca attttaatgt gtgattttat gttgtatttc tgtttgttta aagagatagc
      841 atgattaagg tcaataacac tctgtgtaag attgtattta tgaagttcct ttgcccttat
      901 tgggttagta tatccaagaa tcaatatttc tcctagtatt ccatatcgac gtaaatcaat
      961 cccctcatct attgttgcta ctgcaaatgc ttttacacct atttggtttg cacaagtcgc
     1021 tacttcaaaa gcactatgac cataggcatt agcttttaca actgccatca attcacaatc
     1081 ttctggcata gctttttgta tcatatttac attatgttcc aaattattta aatcaatttc
     1141 aatccaagct ctattggtat ttaccttatg ttctcctttc aagtcttttt ttagatgttt
     1201 aagaatcaat ataataatta tagaagctat aaaagatgct atacttacca ctatataatg
     1261 aataaggcta ttgtcaacca agacagtttg tattccgaat attttagaaa acatacgaat
     1321 aacaacaatc atcataggat gtataatata aataattaat gatgatgttc gcaataacct
     1381 agctcgagaa cctttcaaaa atgttaaggc tgtaaataag aaatacatac atggcaataa
     1441 cataatatac atgctatcat gacgctgtac ccctagttta tgtaaaaaca ttccctcaca
     1501 taacataagt aagaatgata taagaaatcc aatcaagcta tttttagttg acatataatt
     1561 agatttatta gatattattc caccaagtat gaaaaataca ggtgcaaaaa atataccatt
     1621 tcgtgtataa tctgatactt caaaaatata cccatataaa ttctttaata gtggtatttt
     1681 ttctaaaaaa ccataataac tatcaccaaa cataccaatt acataaataa caatagtgat
     1741 tactagtgag tactttaaac ctagtttttt tacaagaaag tatgcaatac ttgcaccaat
     1801 aattgatgcg ggtagatacc ataaatgata taaagtccca tcaaatacaa tgtccttaat
     1861 aatattgggc actaaattat tcatggaaaa atatccagtg taaatattta agggaatata
     1921 aattacaata gctatcccgt aaataaataa tacatttttt agaaaagatt tcagtttatt
     1981 acaattataa ttatatgttg aaattagaaa aaagcctgat agcataaaaa agaatggcac
     2041 agcaatacga gcaattatac tagttaacac aaagtcagcc attttactat atgataataa
     2101 tggagatgta tgaattgcaa ttataagtaa tgcagctata atcctaaaat aatcaagccc
     2161 tgcataagat ttattcctaa tcattcttgt tcctccattt cttagtcctt tctatttctc
     2221 caaaatgtca caataaaacc atccttattg ttacccgata tttgatatac acattgttca
     2281 ggtatagaga ttctcacttc ttctttagta tggataggca cataaaagat ttctataatt
     2341 ttttctttat cttctatttt taaacaattt gtatacgaaa atctcttaat atattctata
     2401 tattcttcaa gagcgagatt cattttattg ataatctcag aatgcggata tcccacataa
     2461 cgaaaatgcc atggttcttg agatattcct gttatttctt cttttttaca ctgataacgc
     2521 tctataaaac cataatgtag tgctgcctta cgaaactcat tacaaatacc attataagga
     2581 aattcagggc agataaaatc tataacttct ttctttaacc ctaaatcaat agcaagccct
     2641 gtctgatgtt cactgtgatt tggaagtgct acaaactttt tagtaaagtc ttttccattg
     2701 tcacgcagag aacttgaata tatatcttgt tgttcctctg cagtacgata tccacttaca
     2761 ggtactattt catttattcc acatatactt tttaaaatat tttgtaaaat gtttgtagct
     2821 tcacgtttca taagaatact tggaaacctt gtatctacag gaattaaatc tatatcttct
     2881 gatgttttaa ctggtaaaaa agcatttact agaattagat tcccatcata aactttatct
     2941 ttagtcaata agatagtatt tgtcatttta tcttctttat ttttatctaa taaaatatta
     3001 ttcatcattt tatgtacacc tcaattatct tgtccaatat ttcttcaaaa gacagtccaa
     3061 tacctttcat catatttgga taacgactat gagaagtaaa gccaggaatt gtattgactt
     3121 cattaaaaat aatttcacca gatggagtta aaaacatatc tactctagca aatccagaac
     3181 atccaagagt tctataaata atttttgctg tctcttgtat tcttttctca caatccaaat
     3241 caattctagc tggcatatga atttttgaac ttttcaatgt atatttttct gtataatcaa
     3301 aaaatccttt tgacaattct atttcatcta ctcttccaac aagtaagtca tcgtttccta
     3361 aaactgcaca ccctacctca aaaccatcaa tattttcctc aattattact tcattatcat
     3421 gttcaaaagc caagtctact gctacttgta actcttcttt tttataaatt ttactaatac
     3481 caaatgatga acctgcacgc acaggtttta taaataatgg ataagttaag tcttttgaca
     3541 gttcagccac attaaattta tctgagtatt ttagtaatat tgattttggt actttaatac
     3601 cttctgaatg aacaagttta tgtgatttgt ctttgtccat acataatgct gaagaaagag
     3661 taccacatcc aataacctgt atacctgcca attcaaataa cccctgcact gttccatctt
     3721 ctccattttt tccatgcagt acaggaaaca caaaatcaag tttagtgtat ttaatatcat
     3781 tttcacatga ttcaatagca ctatttggaa tatgattctg agaaacagca tcaggaatta
     3841 aaaattcaat aataccattt ttgacacggt tctgcgaaac aataactgga attatatcag
     3901 tatccctttc aaaccaagtg ttattctgga tattattata atctccaata taatgatacc
     3961 aattaccctc ccttgtaatt ccaattggta taatatcata tttttccgta ttcaaattct
     4021 caagtactgc aaaagctgat tgtaatgata catcatattc tgttgagttt ccaccaaata
     4081 taactgcaat ttttttatta ttcatctttg attctccttt gttgatttat ttatttttat
     4141 acaatctaca gaaacatgtt aaaatacttt ttctatttca aatccaatca accttcactt
     4201 acaattgcat ctgttccaca taatatattt tcacattttt ctattgatat aattactgta
     4261 tcaaaataaa agcactcttg ttttgataca gtaattatat caataacaag agtgctcttt
     4321 atttatttat cctaaagaat ttcctatttt tttatactta aattcttaca aatttcctac
     4381 aatcggaata ataaccttaa aggtaattat attgttctcg ctatgagctg ttattgtccc
     4441 gttatgaagc tccactattt ctttagctat agcaagtccc aacccagctc taccactgtt
     4501 tgaattacga gatgtatcca acctgtaaaa ttgttcaaaa atacgctcaa gtttttcctc
     4561 tggaatcgta ttgctatgat tcataaattt aagctttata taatcatcct cctgtacagc
     4621 tgttatttca atagtagtat cttcaaaact ataataaaca gcatttcgta ataagttatc
     4681 aaatacacgt tgcattttat taacatcaca ccttatcatc atatttggtg taacattcag
     4741 tgtatatttt aaattcttac tcatcaaagt aggtttaaac tcatatgtta gttgttcaag
     4801 catacgagtt aagcttactg tactgtattc aagtgatata tttgataaat taaaccttgt
     4861 aatttcaaag aattcattaa ttaaatcttc aagacgctct gccttatcca acgaaataga
     4921 taaatatttt tttcgcagtt cttcagaaat ctgactttca tctcgaagta gtgtcagata
     4981 ccctataact gatgtaagtg gtgtttttaa atcatgtgca agataaacaa taagttcatt
     5041 cttacgttgt tctgccattt gtgtctcaaa tttacgttgt tcaagtatat gtttaattga
     5101 gtttattttc ttttctattg ccaataactc tggagataaa gccacttctc caacatcttc
     5161 tttgataaga gagtcaatac cttgatttat ttcactaaaa tattttgtaa accaatttaa
     5221 ataaacacgg aatattataa aaaacacaat caccatagct agtatcatga agagctccat
     5281 gctattacgg aaaatctgct gatataaatt caatgcagct ttatattcca tacgaaaagc
     5341 attttcaaag aatgatacta tccagataga aaacttacca tacaatataa aagaatataa
     5401 aatccatatg aaaataatgg ctataaatgc cattaaagct gttctcataa aaattttatt
     5461 tctcacttga gtaaagtcag gattgacttt aatattttta cttttcaatt gtataaccta
     5521 ctccccatac agtttttata aatttcgggt ttttagaagg ttcatgaatt ttttcacgta
     5581 atcgccctat atgagccata actgtgttat tattatcaag atatttttct ccccatacag
     5641 cttcaaacaa ttcttcagaa ggaaccactt ttccttgatg ttcacataaa taccaaagaa
     5701 tagaaaattc aatcggtgtt aatgctactt ctttcccaaa taaactacat ttatgtgttt
     5761 ctttattgat aatcaatcct ttaatatcat actcattaac aattatcgac tgttgttcat
     5821 aagaattatt atatcgcata tatcttctta attgtgtctt tactcttgca acaacttcca
     5881 aagggttaaa tggctttgtt atataatcat cagcacctat ggttaaaccc attatcttgt
     5941 ctatatcttc aattttagct gttaacataa tgataggata atagtatttt tctcgaattt
     6001 tctggcaaat atgaaatcca tcaatatcag gaagcataat atctaaaata gccatatcca
     6061 aatttttaga ttcaatacat tctagagctt ccttacctgt ataaaacttg tatactgtaa
     6121 aaccttcatt ttttaaatat acttcaatta aatctgctat ttctttttca tcatccacaa
     6181 tcaaaatatt ttcgttcata gattattttt cactctcctt attatcgagg tcatcattta
     6241 taatattggt aaatttacat atcaaataat tacaagcata tttgtaacta atttttataa
     6301 gaaaagaact actacatcag aatgtgttga tgctactatt ggaacacaaa aactgagatt
     6361 aattgttatt ataaaaactc ccctaacacg tccttattat cgcataacag gtattaactt
     6421 tcaataaaaa tatctatcag tcaaacacaa ttcaatttat tataccagag acaatccata
     6481 taaaaaacaa ttaaacctat gtatgtaacc acagacatta ctaaaatttc catgttattt
     6541 tatataaaaa ttcaaaaaag gagttgaatt taaaatttga ttagaaaaaa atcaatttta
     6601 agacaactcc tttttatttt aatgtattta aattatagta tatcagcctt cataatatcc
     6661 tcaaaatcac ctttttcaga tattaatctt atcaatttaa cagtacagct taaatcattt
     6721 ataagtatag ctccaactaa tgaaccatct ttcataaata attttttata tattctttga
     6781 ccttcatctt ttgatgaaat ttctttatca aaattttgta tattaccaat actaaatact
     6841 tttacattca ttgcatctaa tgaaactgga tatacttcac tttcaaatgt caaattatct
     6901 ccacttgcat ttgcccctgc tatttttccc atttctactg aagatggcca tattgctaaa
     6961 ctaatatttc ccacttgtgc aacatctcca caagcatata tatctttaac atttgtctgt
     7021 aatgtcttat ctacaacaat acctctatca atttttacag atgtatcctg aaccatttga
     7081 gtgtttgctc ttacaccaat tgaaaatact accatatcac agtcgacact atctccatct
     7141 ttaaatacaa ctttttgagc ttttccatca ccttcaatac catcaactgc tacacccaat
     7201 cttacatcta ttcctgtatc tcttacacat tgttctaata ttttactacc ttctttatca
     7261 agttgttttg atagtatgct atccatggct tcaactacta ctacttctaa tccttttagt
     7321 ctaaattccc atgcagcttc aagtcccaat aaaccgccac caatcactac aactttctta
     7381 gatttttcta gtgcttcttt tacactatat aaatctttta tatttctaag agtaaacaca
     7441 ttttctaaat catggccctt aattggtggt ataaagtttc tacttcctgt agctattaca
     7501 agcttatcaa atttaatagc tccatcattc actataatag ttttattcac ttcatctaat
     7561 ttatctacat tcgttcctaa tattactact atattatttt tatcatacca atctttatct
     7621 tccatgtaga aatctgaacc aagttcttca tttataccat cagaaagtgc aggtctatag
     7681 taagtcaact catcttcatt agaaatcata gttatcttac aagttttatt tctttttctt
     7741 atagcatctg ctgcataaaa accagctgct ccattaccta ctattacgaa atattcatca
     7801 gtatcttttc taaaagtaac ttcatcttct tcaacttcta taaattggtc ttccttagct
     7861 ccacatacag ggcatacact aggagcacta tcaccttcaa aaacttcatt acaaattaca
     7921 catttaaatt tcttagtttt ctttttactt ccaaaagtag aatttaactt atctaggaat
     7981 ttacctgtat aagaatgtaa tttatcaatc tcttcatttg atggtttaaa gttaacagca
     8041 aaaggtttta ctgctgtcat tctaagctga cttattctct ccatagcatt ttctatacct
     8101 tctccactcc atccatatga accaaatact gaagcaactt ttccaccatg aagaactggg
     8161 tttaacgata ttaatatatc ccatataggc tttaacgcat ctcctaatat tgttggtgtt
     8221 ccaaataata caccttgtga tactgctatt tttgccaaaa tatcttcttg cttatgctct
     8281 attacatcaa ataaatttac tttatagttg ctattttttt caatatatgc ttttatagct
     8341 tctgccataa tctttgtata tccatgtgca gaaacataac aaattgtaac ttctttttct
     8401 aactttattt gttcatttac actccagttg taataaaggt ctataatttt tctaggattt
     8461 tcagttaata ctggaccatg acctggacat acagtatcta tatctaaatc ttttatcttt
     8521 tctatagcag ttaccataga tggtttaaat ggacccatta tacaatcata atagtatctt
     8581 aaagcatcca agtaatcctt ttcttcattt tcatcaagtg tatttactat cttatcatta
     8641 ctatagtgac taccaaatga atcacatgtt acaagagttt tatcctcttt tatatatgtg
     8701 tatatggtat ctggccaatg aagcataggt acagagaaaa attctaaagt tttgtcccca
     8761 attgataatg tatctccatc tgtaactgca acatattcaa agtctttgtt tacgatttcc
     8821 ttaagatatt taattgctgt ttctgaagct actacttttg catttttagc taaatccaaa
     8881 agcttttcaa cacttcctgc atggtctggt tcagtatgac ttacaacaat atagtctatt
     8941 ttttcaaaat ctatatttaa atcatttaat ctttcaatat aattgtcaaa atgcttatct
     9001 ttaactgtct caaataaaac tgttttttct gttcctttta atacatatga attataagta
     9061 gtcccataag gtgtatacat aattatgtca aatactctta aatcagggtc aagtgaacct
     9121 acccaatata aattttcttt tatttttagt gatttcaatg acataccgta ctcctcctaa
     9181 aatagttatt tagtctcttt ataccctgct tgttatattt taaacaaaat ttattcaaaa
     9241 aagaaattat tttaaagata gtttaaatct tctaatcttc tttttctacc ttcaatacag
     9301 ttcccttttt agaatctact tttatgtcat attcattttt atctttatct attcttattt
     9361 tataaaaagt aacatctaaa tctttgtcta aactccattc tttaactatt ccattttgtt
     9421 cttttaatgc aatttccata gcttgttgag gtgtcattat gtcatttaaa tctaattttt
     9481 cttttctagc aactccattt aaatcttctg aatccaactt ttctgtttta tcttctaata
     9541 catcttttgt attagcatca attttcatct tatattcatt attatcatct acaccttcca
     9601 cagtataaac aaatttattt aaatctctct caagacttaa atctactata tcagcatcct
     9661 tatatttatc tttaaatatg tttattgcat ctgtgtatgg cactttaaaa tcttgttcca
     9721 ttaaattttc attatttttt tcatctttta aagcgttatt tgatgcattt gcctcctttt
     9781 ttgtattatc ttgtgcttgt cctgaatccg tagttggact cccactacat gcagttaata
     9841 aacctgctat aagtatactt accactaata aatacgtttt tttacacata aaaatcctcc
     9901 ttaaatatat taatttattc tatattatgc cctctgaacc ctttatttac ccttttatat
     9961 taaaaatttc tcctatttta tcaattttaa aactttatat ttactaaaat taaagttaca
    10021 taaaaattat actgtctcaa aat
//
LOCUS       DAELLV010000132        10000 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.132, whole genome shotgun sequence.
ACCESSION   DAELLV010000132 DAELLV010000000
VERSION     DAELLV010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10000
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.132"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            159..1589
                     /locus_tag="KPD58_002462"
     CDS             159..1589
                     /locus_tag="KPD58_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0266223.1"
                     /translation="MSSQESKPKGNLISNFRDDMRDPKKRAIYTYSSSINLAYNFIGY
                     FVSTVTNIFLTDVAKLGVIMAGGMQIIQSFIKIAVAPITGTVFDKQPFKKGKYYPWLR
                     YAPAALALTYGIFFIVPLMKISPALLLPLMTALLLIASCAQQVVFTIIYAIYPVVAKT
                     PRDRVIGSTTTNIFKEVGKFLVGFSYPLLLVLFADVLGGEGMGYLGTYLFFAAGCLLV
                     FWFSSTEIIKSGAEEEVLQKEEKPKIKASEMAKALFTNVPLMMAFCLEFLICIRSIGL
                     GPLAPYYFKYVIEDERGLAVFLSIMPLVSVAFMFFAPVFIKICREQKLASIISFSICA
                     LCHLLVAWTPWGKTTLGVTMLLAIGGGFSNVVSIINLNFFAGSCDYGHWKSGKDLPGL
                     SMSLYPVAIQVGVLLATTIRTVLMNSMGYQADMVVTEAVKSGFINMISYSMAIPLIIA
                     VVIAILYPVSDKKLNQIREELNQRNA"
     gene            1692..2795
                     /locus_tag="KPD58_002463"
     CDS             1692..2795
                     /locus_tag="KPD58_002463"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBG0266224.1"
                     /translation="MIEKLLRNTVKELHQYVPGEPIEKVKEKYGVKEIIKLASNENPL
                     GPSPKAIEAMIEMLKQGQLYPEPEANELRRKLAEKLDLKPENFIVANGADNVITLIGE
                     AFINRGDEVIYCNPTFPSYRTATIKNEGIPVEVPLTEDYKYDLQGILDKITDKTKLIC
                     VCNPNNPTGTIVDDKELEEFLKKVPENIITILDEAYIEFLTVPNYVDGLKYVRENYNV
                     IVTRTFSKIYGLAGLRVGYGIAKDEIIRTLFTVKEPFSANRVAISGATAALDDEEFIK
                     ETYELNRVGMAYFKEEFTKMGFDVVDSQSNFLYVDMKTDIPKLFEDLKKRGFVIRPSA
                     THARVSIGTMEENRKFVAVLKEILNIKGEPSIG"
     gene            2810..3046
                     /locus_tag="KPD58_002464"
     CDS             2810..3046
                     /locus_tag="KPD58_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG0266225.1"
                     /translation="MKRGVDMNLKINAERCKSCEYCVISCKKGALKISSKINKEGYAH
                     VEVDESKCILCGICYQVCPDNVFEIIKEEACQEA"
     gene            3063..4112
                     /gene="vorB"
                     /locus_tag="KPD58_002465"
     CDS             3063..4112
                     /gene="vorB"
                     /locus_tag="KPD58_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBG0266226.1"
                     /translation="MKKMWKGNHAIAEAALRGGCEFYAGYPITPQTEVMEFLSHRMSE
                     LGRTFIQSENEMAAIYMVYGAYASGMRSMTSSSGPGISLKQEGISYLCANHYPCVIVN
                     VQRWGPGLGSLDSAQTDYLRDTRGGGNGDYHLIVYAPNSIQETVDLMYNSFDVAEKYR
                     VPVEILTEAALGQMMEPVEFPEFKKREGDLGWTYDGSNRDHAKVADNQKPTFCMEKRM
                     RITENEQQWEDYQVEDAEYVFVAFGIPSRTTMNAVRRLREQGEKVGIIRPITVWPFPY
                     KAFEKVSKDVKGFISVESTDTGQLVEDVALASKKVCKENVPVYGLFSGNHIPKTLQVM
                     DTYSKIKSGEIKEVF"
     gene            4114..4854
                     /locus_tag="KPD58_002466"
     CDS             4114..4854
                     /locus_tag="KPD58_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBG0266227.1"
                     /translation="MATEKLTPSVVSIPNKFCPGCGHGIVNRIIAEVIEEHGYEKNHV
                     LTLGVGCVCNMNFSWNGDKMQTAHGRASSTAIGVKVALPDTLVMTYQGDGDAYVIGLS
                     ETLNTAYRNHNVTVFVINNNNFAMTGGQMSWTTMPGQVTTTSVNGRDIKTTGSPLKVP
                     EMVANFFDVAYVARGSVHSPKEIINLKKYIKNAIEAQLNGEGYSLVEILAPCPTNWGV
                     SLEKSIKWMEEEIVPYYALGEFKQRDGE"
     gene            4857..5396
                     /locus_tag="KPD58_002467"
     CDS             4857..5396
                     /locus_tag="KPD58_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBG0266228.1"
                     /translation="MEKELVFAGFGGQGVLTLGLIVAESALELGKQVTWMPAYGPTMR
                     GGKAYSVVKFSDESIGGPDMEEIDVLVAMNKPSLDYINLVKEGGTVVINTSAIDENVT
                     LREDISVVKINCQELAQKVNNPKAANIVVLGALIAKTGLFEKELALKTMCDFFEEKGK
                     GKFNVQNEAAFMEGYNNAI"
     gene            5520..7619
                     /locus_tag="KPD58_002468"
     CDS             5520..7619
                     /locus_tag="KPD58_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HBG0266229.1"
                     /translation="MSLEKLIRPKSIAIVGVTDKLGFGRSAALSIVKSKETDRVYYVN
                     PKREELFGRKCYKTIQEVPEVVDCVVVCTPRNVVPSVLKDSGELGTKAAVVYASGFAE
                     EGTEEGTDLENQLIEISNTYDMKILGPNCMGLLNCIDKVNLWAGGSKWDLYTKKPGIG
                     IVGQSGFITAEIVSSDYFNISYGFSTGNGNIVTLEDAVDFLVDDDYASVIAIYLEGLK
                     NPQKFIDALKRAAQKRKPVIILKSGRSEKGAISAASHTGNLAGSSKAFESIFEKYGVI
                     SVENLEQFMCLAQAFSVLDGNLPANSNFAAINFSGGENTICADLAEENGVELAEISSE
                     TKEEMKKYLPGFATPKNPLDATTALFHEKDMIVGLLHTFEKDDSVGFTMVGANIRDEE
                     NEMHETLCKAVSEAREQGLKKPVFAVPTLEGNRYLDYRTRLEDNQVPIMSSVGTTFTC
                     FNKMAKFIDYDYSKRTLEFKAVKKRESNNVVALSELDSKIEMKKYGVPVPGQGNAKSI
                     DELDEILKYIKYPVVLKINSSEILHKSDVGGVKIGIKNRDEAVDAYNEILTNVKKAKP
                     DANIDGILVQEMVESGIEIIIGITNDDQFGPLLLVGLGGVFVEVFKDTTLYPLPINHD
                     EAIMMLKKLKSFKLLNGYRGSEPCDIDALADMMVKLGKYAYENKDEVKEIDLNPVFVY
                     PKGKGVCAVDALIVKYK"
     gene            complement(7886..8401)
                     /locus_tag="KPD58_002469"
     CDS             complement(7886..8401)
                     /locus_tag="KPD58_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HBG0266230.1"
                     /translation="MISAKMEKLLNEQINHELYSAYLYLSMSSYLEAEGLKGFSNWFY
                     VQYKEETDHAMFFYKYLHNVGGKVQLDAIPMPDSDFTSAMDILERTLAHEKKVTALIN
                     NLAAVANSESDFRTSQFLLWFISEQAEEETNCEDNIKRVKLAGEGGLFFVDQEFANRT
                     YAAPTNPPVTI"
     gene            complement(8493..9704)
                     /locus_tag="KPD58_002470"
     CDS             complement(8493..9704)
                     /locus_tag="KPD58_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HBG0266231.1"
                     /translation="MSNKQGSNIKDVKIKNKLIILKLLSTNTPMSRVDLAKSTGLTKM
                     TLSNLVTELINENMIREIESSTLNAHSSGRKPILLDISNISPCICGILIRRNICKVII
                     SDLKGNIVKQLSETYPKCLSNDVLKDIIKRMFTKIMKINKRDIIGIGISSIGPVDDIN
                     GTILNPPDFGNISNLNIVDFIKEMSDLPIFLINDANSGALAEKMYGLGKNISNYIYLH
                     IMNGIGAGLVLENKLHTGNLGQSGEIGHTSINFSGPLCSCGNNGCLDYYTSVSNLIKR
                     IESLSHIYPNSPLINCKDFSLVKLIDEANNKDSLAMFLLDEFCTYVSYALVNTLTLID
                     CSSIIIGYDFNIPGTFIENTLLKKLTASASFSKYKKISVRHSNFGANAPLIGSIAIVS
                     YNFFNGSINFY"
BASE COUNT         3754 a         1229 c         1849 g         3168 t
ORIGIN      
        1 tatttgacaa ttatgaattt tgagaatata attaaattaa aattatacac atataaaaat
       61 atattcctga atcaagaatg agatatttaa gattcaaaat atatattatt atacgtgaat
      121 aataaaaagt aagaaaattt tataagggag gggtagatgt gagtagtcaa gaaagcaaac
      181 caaaaggaaa tcttatatca aattttagag atgatatgag agaccctaag aaaagagcaa
      241 tatacacata ttcaagttct ataaacttag cgtacaattt tataggttac tttgtatcaa
      301 ctgttacaaa tattttttta actgatgtgg caaaacttgg tgtaattatg gcaggtggta
      361 tgcaaattat acaatctttc ataaaaattg ctgtagctcc tataacaggt acagtatttg
      421 acaaacaacc attcaaaaaa ggaaagtatt atccatggct tagatatgca ccagcagcgt
      481 tagcattaac atatggaata tttttcatag ttccattaat gaaaataagt ccagcattac
      541 ttttaccttt gatgacagct ttattattaa tagcatcttg tgctcagcaa gtagttttca
      601 ctataattta tgctatttac cctgtagttg ctaagacacc tagagaccga gttataggct
      661 caactactac gaatatattt aaagaagttg gtaaattctt agtaggtttt tcatatccgt
      721 tattattagt tttatttgca gatgtattag gtggagaagg aatgggttat ttaggtacct
      781 acttattctt tgcagcagga tgccttttag tattttggtt ttcttcaaca gagataataa
      841 aatctggagc agaagaagaa gttcttcaaa aagaagagaa gccaaagatt aaagcaagtg
      901 aaatggcaaa agctttattc actaatgtac cacttatgat ggcattttgt ttagaatttc
      961 ttatatgtat aagatctata ggattaggac ctttggctcc atattatttt aaatatgtaa
     1021 tagaagatga acgtggatta gcagtatttt tatcaatcat gcctttggta agtgtagcat
     1081 tcatgttttt cgcaccagtg tttataaaaa tctgtagaga gcaaaaacta gcttcaataa
     1141 tttctttttc tatatgtgca ctatgccact tattggttgc atggactcct tgggggaaaa
     1201 ctactttagg agtaactatg ttgctggcaa ttggtggagg tttttcaaat gtagtatcaa
     1261 taataaatct taatttcttt gccggttctt gtgattatgg gcattggaaa tcaggaaaag
     1321 atttgcctgg tttaagtatg tcactatatc cagttgcaat acaagtagga gtattacttg
     1381 caactactat aagaacagtt ttaatgaact caatgggata tcaagctgat atggtagtca
     1441 cagaagcagt taaatcaggt tttataaata tgatttctta ttctatggca atacctttaa
     1501 taatagctgt tgttatagca atactatatc cagtaagtga caagaaatta aaccaaataa
     1561 gagaagagtt aaatcaaaga aatgcttagt taaaagaaag ttaagtagat ggttaattta
     1621 agctattaaa gtaagtagac gcaatagcgt ttatataaat gaaaatacat atacagaggg
     1681 ggcttttaaa aatgatagaa aaattattga gaaataccgt aaaagaatta catcaatatg
     1741 ttccaggtga accaatagaa aaagttaagg aaaaatatgg tgtaaaagaa ataataaaat
     1801 tagcttcaaa tgaaaatcct ctaggaccat caccaaaagc aatagaagct atgattgaaa
     1861 tgctaaaaca aggtcaactt tatccagagc cagaagctaa cgaacttaga agaaaattag
     1921 ctgaaaagct agatttaaaa ccagaaaatt ttattgttgc taatggtgcg gataacgtta
     1981 tcactctaat aggtgaggct tttataaata gaggagatga agttatatat tgtaacccaa
     2041 catttccatc atatagaact gcaactataa aaaatgaagg gataccagta gaagtacctt
     2101 taacagaaga ttataagtat gatttacaag gtatattaga taagattaca gataaaacaa
     2161 aattaatatg tgtatgtaac cctaataatc caacaggaac aattgtggat gataaagagt
     2221 tagaagaatt tttaaagaaa gtccctgaaa atattataac tatattggat gaagcatata
     2281 tagaattttt aacagttcct aattatgtgg atggacttaa gtatgtaagg gaaaattata
     2341 acgttatagt aacaagaact ttctctaaaa tatatggtct tgcagggctt agagtaggat
     2401 atggtattgc taaagatgag ataataagaa ctttatttac agtaaaagag cctttctctg
     2461 caaatcgtgt tgctataagt ggagcaacag cagcacttga tgatgaagag tttataaaag
     2521 aaacatatga attaaataga gttggaatgg cttactttaa agaagagttt acaaaaatgg
     2581 gatttgatgt tgtagattca caatcaaatt tcctttatgt agatatgaaa acagatatac
     2641 ctaagttatt tgaagacttg aaaaaaagag ggtttgtaat aagaccaagt gctacacatg
     2701 cacgtgtaag tattggaact atggaagaaa ataggaaatt tgtagcagtt ttaaaggaaa
     2761 tattaaatat taaaggagag ccaagtattg gataatagat agaaatatta tgaaacgggg
     2821 tgtagatatg aacttaaaaa taaacgcaga gagatgtaaa agttgtgaat actgcgttat
     2881 atcttgtaaa aaaggagcat taaaaatatc aagtaagata aataaagaag gttatgctca
     2941 tgtagaagtt gatgagagta aatgcatact ttgtggaatt tgctatcagg tgtgtccaga
     3001 taatgttttt gaaataatta aagaagaagc atgtcaagag gcttagaagg agttgggata
     3061 atatgaaaaa gatgtggaag gggaaccacg ccattgcaga agcagcccta agaggaggtt
     3121 gtgaatttta tgcaggatat ccaattacac cacaaacaga ggtaatggaa tttttatcac
     3181 atagaatgtc tgaacttgga agaactttta tacagtccga aaatgagatg gctgctatat
     3241 atatggtata tggagcatat gcaagtggaa tgaggtctat gacgagttct tcaggaccag
     3301 gaatatcttt aaaacaagaa gggatttctt acttatgtgc aaatcattac ccttgtgtaa
     3361 tcgtaaatgt acagagatgg gggcctggtc ttggctcatt ggactcagca cagacagact
     3421 atctaagaga tactagaggg ggaggaaatg gagattatca tttgatagtt tatgcaccaa
     3481 acagtattca agagactgta gatttgatgt ataactcatt tgatgtagcg gagaagtaca
     3541 gagtgcctgt cgaaattttg acagaagcag ctttaggtca aatgatggaa ccagtagaat
     3601 ttccagagtt taagaagaga gaaggagatt taggttggac ttatgatgga agtaatagag
     3661 accatgcaaa ggttgcagat aaccaaaagc caactttttg tatggagaaa cgtatgagaa
     3721 taactgaaaa tgaacaacaa tgggaagatt atcaagtaga agatgcagaa tatgtgtttg
     3781 tagcgtttgg tataccatca agaacaacta tgaatgctgt tagaagactt agagaacaag
     3841 gtgaaaaagt tggaataata agaccaataa cagtatggcc tttcccatat aaagcatttg
     3901 aaaaggtaag taaagatgta aaaggattta tctcagtaga atcaacagac acaggtcaat
     3961 tagtagaaga tgttgcttta gcctcaaaga aagtatgtaa agaaaatgta cctgtgtatg
     4021 gtttatttag tggaaatcat ataccaaaaa cattgcaagt aatggacact tatagcaaaa
     4081 taaaatctgg cgaaatcaag gaggtgttct aatatggcta cagaaaaatt aacacctagc
     4141 gttgtttcaa taccaaacaa gttttgccca ggttgtggtc atggaatagt aaatcgtata
     4201 atagcagagg taatagaaga acatggatat gaaaaaaatc atgtattaac attaggagtt
     4261 ggttgtgtct gcaatatgaa ttttagctgg aatggtgata agatgcaaac tgcacatggt
     4321 agagcttcat ctacagctat aggagttaaa gtagcacttc cagatacatt agtaatgact
     4381 taccaaggtg atggagatgc ttatgttata ggtctttctg aaactttaaa tacagcatat
     4441 agaaatcata atgtgactgt atttgtaata aacaataata attttgcaat gactggtgga
     4501 caaatgtcat ggaccactat gccgggtcaa gtcacaacaa caagtgtaaa tggaagagat
     4561 ataaagacta ctggtagtcc actgaaagtt cctgagatgg tggcaaactt ctttgatgta
     4621 gcatatgtag caagaggttc agttcatagc ccaaaggaaa taattaatct aaaaaaatat
     4681 ataaaaaatg caatagaagc tcaattaaat ggagagggat attccttagt tgaaatatta
     4741 gcaccttgcc caactaactg gggtgtatca ctagagaaat ctatcaaatg gatggaagaa
     4801 gaaatagtgc catattatgc tcttggcgaa tttaagcaaa gggatggtga atagttatgg
     4861 aaaaagagct agtatttgca gggtttggtg gtcaaggagt tttaactctt ggtctgattg
     4921 tagctgagtc agcacttgaa ttggggaaac aagtaacttg gatgcctgca tatggtccaa
     4981 ctatgagagg tggaaaagcc tatagtgtag taaagttttc tgatgagagt ataggtgggc
     5041 ctgatatgga agaaatagat gttttagtag caatgaataa accatcttta gattatataa
     5101 atttagttaa agaaggtgga acagtagtta ttaatacatc tgcaatagat gagaatgtaa
     5161 cattaagaga agatataagt gtagttaaaa taaattgtca ggaattagct caaaaagtaa
     5221 acaatccaaa agcagcaaat atagtagtac ttggagcatt aattgctaaa actgggttat
     5281 ttgaaaaaga attagcttta aaaactatgt gtgatttctt tgaggaaaaa ggaaaaggaa
     5341 agtttaatgt tcaaaatgaa gcagcattca tggaaggcta taataacgcc atttgataaa
     5401 ttacccttat cttaagtgat ttatctcaag atttaataat ctttagtgcc aagagaaact
     5461 tcatcttgag ataaaattca cttaaaatca caactataaa catataggag cgtgaattta
     5521 tgagtttgga aaaacttatt agaccaaaaa gtattgctat agtaggagtg actgataaac
     5581 ttggttttgg aagaagtgca gcacttagta tagtaaaaag taaggaaaca gatagagtat
     5641 attatgttaa tcccaaaaga gaagaattgt ttgggagaaa atgttataag acgatacaag
     5701 aagttccaga agtagtagac tgtgtagtag tatgtacgcc tagaaatgta gttccatctg
     5761 tattaaaaga ttctggagag ttaggaacaa aggcagcagt ggtatatgct agtggatttg
     5821 cagaggaagg tacagaggaa ggtacagatt tagaaaatca acttatagaa atatcaaata
     5881 catacgatat gaaaatattg ggtccaaact gcatggggtt gctaaattgt atagataagg
     5941 ttaatctatg ggcaggaggt tctaaatggg atttatatac taaaaaacct ggtataggaa
     6001 ttgtaggtca aagtggattt ataactgctg aaattgtttc aagtgattat tttaatatat
     6061 cttatggttt ttcaacagga aatggaaata tagttacact agaagatgca gtagatttcc
     6121 ttgtagatga tgattatgca tcagtaatag caatttacct tgaaggttta aaaaatcctc
     6181 aaaaatttat agatgcttta aaaagggcag cacaaaaaag aaagccagta ataatattga
     6241 aatcaggaag gtcagaaaaa ggtgctattt ctgctgcttc tcatacagga aatttagctg
     6301 gttcgagtaa agcttttgaa agtatttttg aaaaatatgg agtaatttct gtggaaaatt
     6361 tagaacagtt tatgtgttta gcacaagctt ttagtgtttt agatggtaat ttacctgcaa
     6421 attctaattt tgcagcaata aacttctcag gaggagaaaa tactatttgt gcagatttag
     6481 cagaagaaaa tggtgtagaa cttgcagaaa tatcttctga aactaaagaa gaaatgaaaa
     6541 aatatcttcc aggatttgca acacctaaaa atccactaga tgctacaaca gcattattcc
     6601 atgaaaagga tatgatagta ggtttattac atacctttga aaaggatgat agtgttggat
     6661 ttactatggt aggtgcaaat ataagagatg aagaaaatga aatgcatgaa accctttgta
     6721 aagctgtaag tgaagctaga gaacaaggat tgaaaaaacc tgtgtttgca gttcctacac
     6781 ttgaaggaaa tagatattta gattatagga ctagattaga agataatcaa gtgcctataa
     6841 tgtcatcagt tggaactaca tttacttgtt ttaataaaat ggctaagttt atagattatg
     6901 attattcaaa aagaacttta gaatttaaag ctgttaagaa aagagagtca aataatgtag
     6961 tagcattatc agaattagat agtaagattg agatgaaaaa atatggagta ccagttccag
     7021 gacaaggtaa tgcaaaaagt attgatgaat tagatgaaat cttaaaatat attaagtatc
     7081 ctgttgtttt aaaaataaat tcttctgaaa tattgcacaa atctgatgtt ggaggagtta
     7141 aaataggaat taagaataga gatgaggcag tagatgcata taatgagatt ttaactaatg
     7201 taaaaaaagc aaaaccagat gctaatattg atggtatatt agttcaagaa atggtagaaa
     7261 gtggtataga aattattatt ggtataacta atgatgacca atttggacca ctgttattag
     7321 tcggtttagg aggagtattt gtagaggtat ttaaagatac tacattatat ccattaccta
     7381 taaatcatga tgaagctatt atgatgctta aaaaactaaa atcatttaag cttttaaatg
     7441 gctatagagg aagtgagcct tgcgatattg atgctcttgc agatatgatg gtcaaattag
     7501 gaaaatatgc ttatgaaaat aaggatgaag tcaaggaaat agacttaaat ccagtgtttg
     7561 tatatcctaa aggaaaaggc gtatgtgcag tagatgcctt aatagttaaa tataaatagt
     7621 ttataagttt tggtattcaa ccataattaa agcaccgact gaaaatcaca ttttgtaagt
     7681 tagatagtgt gattttcagt caccccaaat ttaatttaaa aatattttaa aatgaaagta
     7741 tagttttaag tttcattgtg aaatagcact gtataaacaa taaaatacta cgtataaaac
     7801 gaataataaa aggtgtttta atgataagaa tcaatcttga ttttcattaa aacaccttct
     7861 ttattatatt aaaaattttt aaacgttata ttgtcacagg tgggtttgtt ggagctgcat
     7921 atgtacgatt agcaaattct tggtcaacaa agaatagacc accctctcca gcaagtttaa
     7981 cacgttttat attgtcttca caatttgtct cttcttctgc ttgttcagat ataaaccaaa
     8041 gtaagaattg agaagttcta aagtctgatt cagagtttgc tacagctgct aagttgttta
     8101 ttaaagctgt aacctttttt tcatgagcta acgtacgctc aagaatatcc attgcagaag
     8161 taaagtctga atctggcatt gggatagcat caagctgaac ctttcctcct acattatgta
     8221 agtatttgta aaaaaacata gcgtggtctg tctcttcttt atattgaaca taaaaccaat
     8281 tagaaaatcc tttaagtcct tctgcttcaa ggtaagaaga catagaaaga taaagatacg
     8341 cagaatataa ttcatggtta atctgctcat taagtaattt ttccatttta gctgaaatca
     8401 tttttatacc tccaaatttt aaatgctctt ataaaatata ataatattaa atggaaaaat
     8461 attcaataaa agtgtttaaa tattttacat atttaataaa aatttatact accattaaaa
     8521 aaattataac ttacaatggc tatagagcca attaatggtg catttgctcc aaaatttgaa
     8581 tgccttacac ttattttttt atatttagaa aaacttgctg aagcagtcaa tttttttaat
     8641 aatgtatttt ctataaatgt accaggtata ttaaagtcat agcctattat tatagaacta
     8701 caatcaatca atgtaagtgt attaactaat gcatatgata cgtatgtaca aaattcatca
     8761 agcaaaaaca tggcaagact atctttatta tttgcctcat ctattaactt gactaaagaa
     8821 aaatctttac agttaattaa tggggagtta ggataaatat ggctcaatga ttcaattctt
     8881 ttaattagat tactgacact tgtataataa tctaaacatc cattgttacc acaactgcac
     8941 aatggtccac taaaattaat tgatgtatgt cctatttctc cactttgtcc taggttgcca
     9001 gtatgaagtt tattttctag aactagacca gctccaatac cattcattat gtgtaggtat
     9061 atataatttg atatattttt acctaaacca tacatttttt ctgctaatgc acccgaatta
     9121 gcatcattta tcaaaaatat tggcaaatca gacatttctt ttataaaatc tactatattt
     9181 aggtttgata tatttccaaa atctggggga tttagtatgg taccatttat atcatctact
     9241 ggacctattg aagaaatacc aattccaatt atatctctct tgttgatttt cattatttta
     9301 gtaaacattc tttttattat atcttttaaa acatcatttg atagacattt agggtaagtt
     9361 tcagaaagtt gttttacaat attacctttt aaatctgaaa taattacctt acatatattt
     9421 cttcttatta ggattccaca aatacaaggc gatatattag aaatatctaa taaaatgggt
     9481 ttacgacctg atgaatgtgc atttaaagtt gatgactcta tttccctaat catattttca
     9541 ttaataagtt cagtaactag atttgataaa gtcattttag taagtcctgt agacttagct
     9601 aaatcaactc tggacatagg tgtatttgta gaaagtaatt tcaatattat taacttattt
     9661 ttaattttta catctttgat gttagaacct tgtttgttac tcatcataaa tctccttaaa
     9721 taaagttaag tgttttattt attttcaagt atactatcac cttataatag agtaacattt
     9781 ttttacttga aaatatcatt aattagaata tatcacatta tatatcataa aacatataaa
     9841 aaaatattag aattaaatta aaagtgaaaa gtagattgat atataaatag taaatactta
     9901 attaaaaaaa gataatgata gcaataatta ttgttaccaa gaatctacta cttcaagtgt
     9961 taccttaggt atttaagttg atagtggttt gcacgtaata
//
LOCUS       DAELLV010000133         9981 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.133, whole genome shotgun sequence.
ACCESSION   DAELLV010000133 DAELLV010000000
VERSION     DAELLV010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9981
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.133"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1156
                     /locus_tag="KPD58_002471"
     CDS             <1..1156
                     /locus_tag="KPD58_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG0266232.1"
                     /translation="NEMFGYAEGNKILIHISQVLNNNTNKYEICARMDNDHFILLIAC
                     NTEDELLNRLSKINKEICNLNTTNSSKYKIVMSSGIYKITKKDDIKKIDLLIDRANIA
                     AKSKKEKYEHSYSFFNEDTRNRLYKEKRLEDNMNKALEKGEFIVYYQPKYSLDDVNEI
                     EGAEALIRWNSPEFGFISPIDFVPLFEKNGFIVNIDMFVFEEVCKTLNKWINKGYTPV
                     PISVNMSRVHLYRDNFIENITDLISKYNISPEFIELELTESVVFDNLNILIDIMKKIK
                     EIGFLISMDDFGSGYSSLNLLKDLSFDILKLDRGFLIETTDTKRGKIIISKIVEMAKA
                     IDIKVICEGVETYEQVEFLKEIGCDKVQGYLFAKPMVLDEFEKHLNFKFD"
     gene            1303..2049
                     /locus_tag="KPD58_002472"
     CDS             1303..2049
                     /locus_tag="KPD58_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0266233.1"
                     /translation="MNILSIRNISKTYCGNIPFKALDKVSLNIEKGEFVSVMGPSGSG
                     KSTLLNIISTVDRQSEGEVVLDGYDVSKLKGEKLAEFRRKQLGFVFQDFNLIDTLTVG
                     ENIMLPLTLEGASIKDMNIQTKSISKFLGIDKILDRKTYEISGGQAQRCAIARAIINK
                     PAILLADEPTGNLDSKSTDDVLKLFTRINKEQKVTTLMVTHEAYSASYSDRVIFIKDG
                     CIYTEIKKSESSNSFYSDILAVLSQIGGVR"
     gene            2049..3929
                     /locus_tag="KPD58_002473"
     CDS             2049..3929
                     /locus_tag="KPD58_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0266234.1"
                     /translation="MDFRKFAYNNIVRNFRAYLAYFLSSVFSIMIFFVFAVSMFHPII
                     TKSGMQSGSTAFMALMTSELIIFGFSLLFILYSLGNFLKYRLKDFGVLMIIGMSNRQL
                     KKLILIENLIIGFLAVMLGILLGLSISKLFLLYLSKLFYMNLTEFYFPVKAMLLTIVS
                     FMILFLITGPMSLKLLSKKSILELLVGTKKPKKEIKSSKLFGIIGVICLILGYAMILF
                     GNKLKINGGMYTTALLITLGIFLFFSQFTILILKYLKNKEKFYKNKVNMLLISNLIYK
                     LKDNTRLLFLITILLSATLVAFTTTSTLVSSQGEASKNNFPMVYSYFSEHNNEKEYEE
                     LQQIREAIKGYDYKELSFPVLKYNREVLLSVKNYNKLSKELGLDKISSKLEKYEGIII
                     PSSNSQEHINSLKQLKNYSIKNLNIEVKKSKNKIIFPTGLFSKVVVISDDLYNEVKGE
                     FSKINFHGFDYKNWQSSAKITENLKEKHFNLDREYNRSYFLTLPDMYLVELQQSKILQ
                     FMGVFIGVILFIAVFSFLYFRLYTDEIEDNVKYKKLSKIGLSFKNMNKIVSIEIGTLF
                     FIPYFIAIINSIISLLFLNQVLNNSFKLENLGILLLISSIYSLYFYLLKKNYTRKVWF
                     FD"
     gene            4048..5439
                     /locus_tag="KPD58_002474"
     CDS             4048..5439
                     /locus_tag="KPD58_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG0266235.1"
                     /translation="MENQQLLGTERISKLLLKYSIPAIIGMLVNSLYNVVDRIFIGNI
                     PGVGPLAITGLGVTMPIMTIILAFGMLIGIGTTTTISIKLGQGKVEEARKLIGNAMTL
                     SVITGIIIMILGILFANKILTLFGASENTLIYAKSYINIILLGTVVNLLSFSLNHSIR
                     ADGSPKISAGIMIVGCLTNIVLDWILIFGFNLGIQGAAIATVTSQALTAILTIGYYIS
                     GKSNLRFSKSNLKLDKKLIKAVFAIGMSPFAMQLAASLVQVISNIALKTHGGDLAIGA
                     MATISSIAMVFLMPIFGINQGAQPIIGFNYGAEKYDRVKKAYLGSLVVATIILCMGMV
                     VVMLFPEAIIGIFNKDPELMNISVNGLRIYLLMLPIVGLSVTGTNFIQSIGKAKMAML
                     LSLLRQVILLIPAVLILPTFLGLQGVWTAQPVSDFIATVITGIVVFRELKRYTPKTEK
                     LNENERLNEITTE"
     gene            5546..6586
                     /locus_tag="KPD58_002475"
     CDS             5546..6586
                     /locus_tag="KPD58_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBG0266236.1"
                     /translation="MEFTKKSKLKNMYVNMNVARMLSKINEYKGRQLLYKKQPKEILE
                     NLEKKSLVDCSESTYIGNQRDSSNFNLEKLISNEVTPRSREELSIVEYRDVVKTINSA
                     YESIPISSQTILELHGYLYKFSSTRGGSYKSDNDFIEHNLKPSEFISTDSSVNKVEKA
                     VEEICEAYNTLIEEDEIDILILISAFVLDFILIHPFKEGNIKMARVLILLLLNKNGYE
                     VGRYISLGKIFDDSSYEYYSNLNYLKASIGSEKADMNAWIEYFLETILTAYEKLDDSL
                     NISDKKRQTKTSRIEKIINSTLGYFTKEDIRDLCPDIPEPTINRVFNNLRKQDKIEVV
                     ARGRSAKWKKKY"
     gene            7075..7728
                     /locus_tag="KPD58_002476"
     CDS             7075..7728
                     /locus_tag="KPD58_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBG0266237.1"
                     /translation="MKKKLLYINVNSKPEDLSSSKTVARKFINKFMEKNKDFEVEEID
                     LYKEHIPRLEYQYFEKRNSIVSEENAKNLDDKDRKELTKIRNLCDQFVNASVYVIAAP
                     MWSLSFPAPLKEYIDCIIQDGKTISFEGNDKPQGILNDIDRSMVYIQSSGGHIPWVLK
                     PVMNKGLNYVESIMKFIGIKKFDELLVDGTGTSEEERQAAIEKASEKIDGIIDGMKF"
     gene            7804..8013
                     /locus_tag="KPD58_002477"
     CDS             7804..8013
                     /locus_tag="KPD58_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266238.1"
                     /translation="MREEKSNEKYDCYWCNQENNFCVEIKDNIVMIDDGTGTLKQAVF
                     IGYKQIQINLNCSHCQNLNRIKLNL"
     gene            complement(8153..8719)
                     /locus_tag="KPD58_002478"
     CDS             complement(8153..8719)
                     /locus_tag="KPD58_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HBG0266239.1"
                     /translation="MMNNELNTIILETLNNADITSNDIPSIDLYMDQIISLIDNKLSA
                     NKRFESDKILTKTMINNYSKEGLIKPVKGKKYTKEQILQMIIIYSMKNTLTIQEIKRI
                     LHGVYEKDNFSEKDLVSCYEKFMLIKENQRKNIPDFIESNFENISINPENKDDLLIAL
                     LSLTSMADQLKNISEKLVDRYFPDITKK"
     gene            8880..9533
                     /locus_tag="KPD58_002479"
     CDS             8880..9533
                     /locus_tag="KPD58_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBG0266240.1"
                     /translation="MYQFFLKGRDPISSLTHFIGACLSLLATIILVFQSVTLQETSLL
                     MIVSVSVFGLSLIALYSASSYYHFLKGTPEQELFFRKVDHAMIYVLIAGSYTPICLNF
                     MEKKEGIIFVTAIWIVAFIGIIIKIFWMDAPRWLSTSIYLLMGWAIVFDINAFNSIPK
                     DCLRLLIMEGVSYSIGAIIYIIKKPNISPEFGFHEIFHIFIMIGSLFHFLAVLLYVL"
     gene            complement(9691..9867)
                     /locus_tag="KPD58_002480"
     CDS             complement(9691..9867)
                     /locus_tag="KPD58_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266241.1"
                     /translation="MYKYDDYVNLTNDLDLPVEIRNKCVRIVSVSENLEDIIIVHKFK
                     LYVVNEKYIKGIME"
BASE COUNT         3886 a          995 c         1593 g         3507 t
ORIGIN      
        1 aaatgagatg tttggatatg ctgaagggaa taagatatta attcatatat cacaggtttt
       61 aaataataat acaaataaat atgaaatttg tgctaggatg gataatgacc attttatatt
      121 gttgattgct tgtaacactg aagatgaact tttaaataga ttatctaaaa taaataaaga
      181 aatttgtaac ttaaatacta ctaactcatc taagtataaa attgttatgt caagtggaat
      241 atataagatt actaaaaagg atgatattaa aaagattgac ttgttaattg atagagcaaa
      301 tattgcagca aaaagtaaaa aggaaaaata tgaacattca tattcatttt ttaatgaaga
      361 tacaagaaat cgattgtaca aggaaaaacg attggaagat aatatgaata aagctttaga
      421 aaaaggtgaa tttatagtat attatcaacc taagtatagt ttagatgatg taaatgaaat
      481 tgaaggagca gaagccttga taagatggaa tagtccagaa tttgggttta tttcacctat
      541 agattttgta cctttatttg aaaagaatgg ttttattgta aatattgata tgttcgtgtt
      601 tgaagaagta tgcaaaacat taaacaagtg gataaataaa gggtatactc ctgtaccaat
      661 ctctgtaaat atgtctagag ttcatttata tagggataat tttatagaaa atataactga
      721 tttgataagt aaatacaata tatctcctga gtttattgag cttgaactaa cagaaagtgt
      781 tgtgtttgat aatttaaata tattgattga tataatgaaa aagattaaag agataggatt
      841 ccttatatca atggatgatt ttggttcggg atattcatct ttaaatctgt taaaagacct
      901 ttcttttgat attttaaaat tagatagagg atttttaatc gaaacaacag atacaaaacg
      961 tggaaaaatt attatttcta agattgtgga aatggcaaaa gcgattgata ttaaagtaat
     1021 ctgtgagggc gtagaaactt atgaacaagt agagttcttg aaagagatag gttgtgataa
     1081 ggttcaagga tacttatttg ctaaacctat ggtattagat gaatttgaaa aacacttaaa
     1141 ctttaaattt gattaatcat taataattat tagactataa gataccctaa caacttactt
     1201 acaaatatgt aagttaattg ttagggtatt tttatggtta aagtagtata ttcacgatat
     1261 tatggtattg tagtaaaaat aaaaaattat ggagaatata aaatgaacat attgagtatt
     1321 agaaatatat caaaaacata ttgtggaaat attccattta aagcattaga taaggttagt
     1381 ctaaatatag aaaagggaga atttgtgtct gttatgggac catctggaag tggtaaatct
     1441 actcttttaa atataatatc tacagttgat agacagagtg aaggtgaggt tgtattagat
     1501 ggatatgatg taagtaaatt gaaaggagaa aaacttgctg aatttagaag gaaacaactt
     1561 ggttttgtat ttcaggattt taatttgatt gacactttaa ctgttggcga aaatattatg
     1621 ttgccactta cactagaggg ggcaagtatc aaagatatga atatacaaac taaaagtata
     1681 tctaaatttt taggaataga taaaatttta gatagaaaaa cctatgaaat ttcaggaggt
     1741 caagctcaaa ggtgtgctat tgctagagct attataaata aacctgccat acttcttgca
     1801 gatgagccaa ctggaaatct tgattcaaaa tctactgatg atgttttgaa attatttact
     1861 aggataaaca aagaacaaaa ggtaacaaca cttatggtaa ctcatgaagc atatagtgca
     1921 agttattcag atagagtaat ttttataaaa gatggttgta tatatacaga aataaaaaaa
     1981 tctgaaagca gtaattcttt ctattctgat atactagctg ttttgtctca aataggaggt
     2041 gtaagatagt ggattttaga aagtttgctt ataataatat agttagaaat ttcagggcat
     2101 atttagcata ttttttaagt tctgtttttt ctataatgat attttttgtt tttgcagtgt
     2161 ctatgttcca tccaattata acaaaatctg gaatgcagag tggttctact gcatttatgg
     2221 ctcttatgac ttcagaatta ataatattcg gttttagtct actttttata ttgtactctt
     2281 taggaaattt cttaaaatat agattgaaag attttggagt attgatgata attggaatga
     2341 gtaacagaca actgaaaaaa ttgatattaa ttgaaaattt gataatagga tttttagcag
     2401 tgatgttagg aatattactt ggtttatcaa tttcaaaatt gtttttattg tatttaagca
     2461 aactgtttta tatgaattta actgaatttt attttccagt aaaagcaatg ctattgacta
     2521 ttgtatcatt tatgatttta tttttgataa caggaccaat gagcttaaaa ttattgagta
     2581 aaaagagcat tttagagctt ttagtaggta caaaaaagcc taaaaaagag ataaaatctt
     2641 caaagctatt tggaataatt ggtgtaatat gtcttatatt aggatatgct atgatactat
     2701 ttggcaataa gttaaaaata aatggaggta tgtatacaac agcactatta attactttgg
     2761 ggatattttt atttttttct caatttacta ttttgatatt aaagtattta aaaaataaag
     2821 aaaaatttta taagaacaaa gtaaatatgt tacttataag taatttgata tataaattaa
     2881 aggataatac acgattgtta tttttaataa caatactttt atcggctact ttagtagcat
     2941 ttacaacaac ttcaacactt gtaagttctc aaggagaagc ttctaaaaat aatttcccaa
     3001 tggtttactc atatttttca gagcataata atgaaaaaga atatgaagaa ctacaacaaa
     3061 ttagagaagc aatcaaagga tatgattata aggagttaag ttttcctgta cttaaatata
     3121 atagggaagt cttactaagt gtaaagaatt ataacaaatt atcaaaggaa ttgggattag
     3181 ataaaattag ttcaaagtta gaaaaatatg aagggattat aattcctagt agcaattcgc
     3241 aagaacatat aaattcttta aaacaattaa aaaattatag tataaaaaat ttaaatatag
     3301 aagtaaaaaa atcaaaaaat aaaataattt ttcctacagg actgtttagt aaagttgtgg
     3361 ttataagtga tgacttatac aatgaagtaa aaggtgaatt ttcaaaaatt aattttcatg
     3421 gatttgatta taaaaactgg caaagcagtg caaagataac tgaaaattta aaagaaaaac
     3481 attttaattt ggatagagaa tataacaggt catatttttt aacattacca gatatgtatt
     3541 tagtagagtt acaacaaagt aaaattcttc aatttatggg tgtttttata ggagtgatat
     3601 tatttatagc agtttttagt tttttatact tcagattata tacagacgag atagaagata
     3661 atgtaaagta caaaaaacta tcaaaaatag gtctatcttt taaaaatatg aataagattg
     3721 ttagtataga gataggtaca ttgtttttta taccgtattt tatagcaatt ataaatagca
     3781 taatttcttt actgttttta aatcaagtat taaataatag ttttaagtta gaaaatttgg
     3841 gtatattact actgatatca tctatatatt cactttattt ttacttatta aaaaaaaatt
     3901 atactagaaa agtttggttt tttgattaaa gtggtatata atagatatat aaattaatat
     3961 ttaaaaagta gttaatatta ggggagggca tttgcacctc ccttgtaata tgtggaaaat
     4021 tgaatattag aagaaggagg agacttgatg gaaaatcaac aattattagg aacagaacga
     4081 attagtaaat tactactaaa atattctata ccagctataa taggtatgtt ggtaaatagt
     4141 ttatataatg tagtagatag aatttttatt ggaaatatac caggggttgg accacttgca
     4201 ataacaggtc ttggtgtaac aatgccaata atgacaataa tacttgcatt tgggatgttg
     4261 ataggtatag gaactactac taccatatca ataaaacttg ggcaaggtaa agtagaagag
     4321 gcaagaaaac ttataggaaa tgcaatgacg ttatctgtaa taacaggaat tatcataatg
     4381 atacttggaa tattatttgc aaataaaata cttacattgt ttggagcaag tgaaaatact
     4441 ttaatatatg caaaatcata tatcaatatt atattattag gtacagtagt aaatcttctt
     4501 tccttttctt taaaccactc aataagagct gatggtagtc ctaaaatttc agcaggaata
     4561 atgatagtag gatgtttaac taatatagtt ttagactgga tattaatatt tggattcaat
     4621 ttgggaatac aaggagctgc aattgctaca gtaacttctc aggctcttac agctatttta
     4681 actatagggt attatataag tggaaaatca aacttacgat tcagtaaatc taatctaaaa
     4741 ttagataaaa agctaataaa agcagtgttt gcaataggta tgtcgccatt tgctatgcaa
     4801 cttgcagcaa gtttagttca agtaatatca aatattgcac ttaaaactca tggtggagat
     4861 ttagctatag gggctatggc tactatttct tctattgcaa tggtattttt gatgcctatc
     4921 tttggaataa atcaaggagc gcaacctatt ataggtttca attatggagc tgaaaaatat
     4981 gatagggtta aaaaagcata tttaggttca ttagtagtag ctactataat tttatgtatg
     5041 ggtatggtag tagttatgct tttcccagaa gctattatag gtatatttaa taaagaccct
     5101 gaacttatga atatatctgt taatggatta agaatatact tacttatgtt gccaattgta
     5161 ggcttatcag ttacaggaac aaactttata caatctatag gaaaagcaaa aatggctatg
     5221 ttattgagcc ttttaagaca agtaatttta ttgataccag cagtattaat tcttcctaca
     5281 ttcttaggtt tacagggtgt atggacagct caaccagtgt ctgattttat agctacagta
     5341 attacaggta tagttgtatt tagagaatta aaaagatata ctcctaaaac tgaaaaatta
     5401 aatgaaaatg aaagactaaa cgagataact actgagtaaa aatataaatt cagataattg
     5461 agcaagaaat tgcaaaaaat acgattatat gataaaataa aaccgctgtc aaggataaga
     5521 gaaattctaa ttatggagtg attttatgga atttacaaag aaaagcaaat taaaaaatat
     5581 gtatgtaaac atgaatgtag ctagaatgtt gtctaagata aatgagtata agggtagaca
     5641 actgctttac aaaaaacaac caaaagaaat tttagaaaac ttagaaaaaa aatctttagt
     5701 agattgttct gaatctacat acattggaaa tcaaagagat agtagtaatt ttaatctaga
     5761 aaagttgata agtaatgaag ttacacctag aagtagagag gaattgagta ttgttgaata
     5821 cagagatgtg gtaaaaacta ttaacagtgc ttatgagagt attcccatca gttcacaaac
     5881 aattcttgag cttcatggat acttatataa attttcatct actagaggag gaagttataa
     5941 gtcagataat gattttattg aacacaattt aaaacctagc gaatttataa gtactgattc
     6001 aagtgtcaat aaagttgaaa aagctgtaga agaaatatgt gaagcttata atacattgat
     6061 agaggaagat gaaattgata tattaatatt aatttctgca tttgtgttag atttcatatt
     6121 gatacatcca ttcaaagaag gtaatataaa aatggcgaga gtacttatcc tattgttact
     6181 aaataaaaat gggtatgaag ttggaagata tataagttta ggaaagatat ttgatgatag
     6241 ttcatatgaa tattatagta atttaaatta tttgaaagcc tcaataggta gtgaaaaagc
     6301 agatatgaat gcatggattg agtatttttt agagacgata ttaactgcat atgagaagct
     6361 ggatgatagt ttaaatatat ctgataagaa aagacaaaca aaaactagta gaatagagaa
     6421 aataataaat tctacccttg gatattttac aaaagaagat ataagagatt tatgtcctga
     6481 cattccagaa cctactataa atagagtttt taataatcta agaaaacaag ataagataga
     6541 agttgttgca agaggaagaa gtgcaaaatg gaagaaaaaa tattaaaaaa tgtttagata
     6601 actaaaatgg tatagtgttt tacactatac catttttaat gtcattagca tgagcaataa
     6661 tttaacaact taagcaatat caattttatt tattgattat ttttgaactg aaaaatggcg
     6721 ttatatggta tacctcagtg gtaattaatt ttggatgaga agatataata ttacagaata
     6781 tattaggagt atatgtagaa aaaatataat aaaagtgtga ggcttagtaa ttaacaaatt
     6841 atgtggcttc acactttttt ataaaataaa gatacttagc agaataatat tttagttgat
     6901 atgtttataa gatggtactt gtttgggtaa aagtataaat agtaaaaaag tagaagtaat
     6961 gtgtataaaa acataagtta cattctgtaa taatagttat tttataattc aacaaaagaa
     7021 tagaatgggt atatttgtga aataataata taaaataatc ttaggagtga gcttatgaag
     7081 aaaaaattat tatatataaa tgtaaattca aaacctgaag acttatcttc aagtaaaact
     7141 gtagcaagaa aatttataaa taaatttatg gaaaaaaata aagattttga ggttgaagag
     7201 atagaccttt ataaagaaca tataccaaga cttgagtatc agtactttga aaaaagaaat
     7261 agcatagtta gtgaagaaaa tgctaaaaat ttagacgata aggatagaaa agagcttaca
     7321 aagattagaa atctttgtga ccaatttgtg aatgcaagtg tttatgtaat agcagcacct
     7381 atgtggagtt tatcttttcc agcaccactt aaagagtata tagattgtat aattcaagat
     7441 ggaaaaacta tatcttttga aggcaatgat aaaccacaag gaattttaaa tgatattgat
     7501 agaagtatgg tatatataca atcttctggt ggacacatac catgggtgtt gaagccagtt
     7561 atgaataaag ggttaaatta tgtggaaagt attatgaaat ttataggaat taaaaaattt
     7621 gatgaattgt tagtagatgg aactggaact agtgaagaag aaagacaagc tgcaatagaa
     7681 aaagcatctg aaaaaataga tggtataata gatgggatga aattttaatt ataatatttt
     7741 attaaaaata taaaggaaaa acctctttta atgtagaaac ttatattgaa ggaggcgaga
     7801 atcatgcgag aagaaaaaag taatgaaaag tatgattgtt attggtgtaa tcaagagaat
     7861 aatttctgtg tagaaataaa agataatata gtcatgatag atgatggcac tggtacgtta
     7921 aaacaagccg ttttcatagg gtataaacaa atccaaatta atttaaactg ttcacattgt
     7981 caaaacttaa atagaataaa attaaatttg tagaatttta aaatggaaat tattgaagga
     8041 attaaaaata gaaagtgtta atatttagac tgaaaattat attttataca ataaaagttt
     8101 atttaaataa cattgtattt taatatttat tttcagtctt actttattaa aattattttt
     8161 tggttatgtc tggaaaatat ctgtctacta atttttctga aatatttttt aattggtcag
     8221 ccattgaagt cagacttaat aaggctatca ataaatcatc tttattttct ggatttatac
     8281 taatattttc aaaattagac tctataaagt ccggtatatt ctttctttga ttttctttta
     8341 ttaacatgaa tttttcgtaa catgaaacta aatctttttc actgaaatta tccttttcat
     8401 acacaccatg taagattctt ttaatttctt gtattgtaag tgtatttttc atagaataaa
     8461 taattatcat ctgtaaaatt tgttctttag tatacttttt tcccttaact ggtttgatta
     8521 atccctcttt gctgtaatta tttatcatgg ttttagtaag aattttatca ctttcaaatc
     8581 ttttattagc agaaagctta ttgtcaatta gagaaataat ttgatccata tataagtcaa
     8641 tagatggaat atcgtttgaa gttatatctg cattatttag tgtttctagt attatagtat
     8701 taagttcatt attcatcata tgttacctcc ataatatata taatacacta aattagaaag
     8761 taaaacaata ttaaggcttg taaaaaaaac ataaagtggt ataattaaat atataatata
     8821 gtaatcaaaa ctatatttat gaatctgatt tttaaaatac ataattgtag gaggacgaaa
     8881 tgtatcaatt ttttttaaag ggtagagacc caataagcag tttaactcat tttataggtg
     8941 cttgtttatc tcttttagca actataattt tagtttttca atctgttaca ttacaggaaa
     9001 cttctctctt gatgatagta tctgtatctg tatttggact ttcactaatt gctttatata
     9061 gtgcaagttc gtattatcat tttttaaagg ggacaccaga acaagaattg ttttttagaa
     9121 aagtagacca tgcaatgatt tacgtattga ttgctggctc ttatacacca atatgtctga
     9181 attttatgga aaaaaaagaa ggcataatat ttgttacagc aatatggata gttgcattta
     9241 ttggcataat aataaaaata ttttggatgg atgcacctag atggttatct accagtatat
     9301 atttacttat ggggtgggca attgtttttg atataaatgc attcaattca attcctaagg
     9361 attgccttag actattaatt atggaaggtg tatcttactc tataggtgcg attatttata
     9421 taattaaaaa gcctaatata agtcctgagt ttggatttca tgaaatattt catattttta
     9481 ttatgatagg ttctttattt cactttttag cagttttatt gtatgtttta tagttttaaa
     9541 agatttgatt aattgatact aaaattctac aatgaataaa atggaactgc cttatataga
     9601 ttatatctta gacaataaga cagttcctta ttataaatat taaattctac ttataataaa
     9661 attttgataa aaatagtcca ttcaaaatgc ctattccata attcctttaa tgtacttttc
     9721 atttacgaca tatagtttga atttatgtac tataattata tcctctaaat tctctgaaac
     9781 actaacaatt ctgacacatt tgtttctaat ttctacaggt aaatctaaat catttgttaa
     9841 attcacatag tcatcatatt tatacataaa atcatccctt tacattaaga tatacttatt
     9901 atactatatt ttaataagtt tttttaaatt attacaattt ttcccgaaat ttcatttttt
     9961 atacataaaa tttccaaatt a
//
LOCUS       DAELLV010000134         9946 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.134, whole genome shotgun sequence.
ACCESSION   DAELLV010000134 DAELLV010000000
VERSION     DAELLV010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9946
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.134"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(35..2692)
                     /locus_tag="KPD58_002481"
     CDS             complement(35..2692)
                     /locus_tag="KPD58_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0266242.1"
                     /translation="MIYKKDKVMKVLESSYRENEKGITANQLSEKTGIARNNISTYLN
                     QLFNEGMVTKIKGRPVYYIPANLNLNKNELLDTQSELLGIKKGTKVENKIKKDSFETL
                     IGKEHSLRPIIEKCKVAMLYPTNGLHTIIYGETGVGKSMIARYMYDYSIDSGIRKNKA
                     PFVTFNCADYANNAQLLMGHIFGVEQGAYTGAESSRKGLLEIANGGILFLDEIHRLPA
                     EGQEMLFTFIDKGTFKRLGDATKERESKVLIICATTENPTSTLLDTFNRRIPMKIEIP
                     SLRDRTMIERMELVKNFFKEESKSISISIKVHKEIIKSLLLYDCKNNVGQLQNDIKLA
                     VANGYLRYKRDNKEPVYIEKKYFDKSINEVSDDYREKSIKADEVITHGIEYFVFTSLG
                     EEEVINYNDINIINAVNTNLNKSVEMNIADASLFNNVKFKSMCESIRQIIKEDEEIDI
                     SESIFKSMAIYIKSVLDKSSSTNNIDLNQIRRNNRSEFKTALKIVGIIENEFDLFLSI
                     ETVAYITLFIVQAKQDEQNKIVNNINIIVAMHGETTASSMVKAVGDIIGKCSAISFDM
                     KLDKSYDEVIVDFKNLINNIDNKDVLLFTDMGSLNSFDEIIKKEKKCGVRVIPMVTTL
                     TVLEAVQKANMGLPLNDVYNSITNTRKYYFGTNEIQNKENLSKTIIIASHVSEGVDNK
                     TRKILEEKMSRYLDGIDIISVPYKTEKDLSLNITKLKESSNIVAVINEQRINIRGIDY
                     ISKKDIDKDENINKLKNIIKISIGYDDVVEGLKTSLKSSNYNRIFKGIKYVSDELFLV
                     FNIEKKYDKVIGLMMHLAFMVDGLIGNTREIEKLDKEKTLDYHKSLSKIKDIVSQLDK
                     KYNIEINEKECYQILLILEYAEIIEKDYQ"
     gene            complement(3051..3995)
                     /locus_tag="KPD58_002482"
     CDS             complement(3051..3995)
                     /locus_tag="KPD58_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009527644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0266243.1"
                     /translation="MNREKILEIKNLKQYFHLDKSTTVKAVDDISFDIYKGEIFGLVG
                     ESGSGKSTTGKTIIRLHESTGGEVIYKGNCISDKKTYKFIKKDVNKSMQIIFQDSTSS
                     LNPRMTIADIISEPLKIQGICKNKTDRMNKVYEMLKLVGLDRSYANKYPSDFSGGQRQ
                     RIGIARALSVDPEFIIADEPIASLDVSIQAQIVNLFKKLQQEKNLTCLFIAHDLSMVR
                     HISDRIGVMYNGKLVELADSNELYNNPIHPYTKSLLSAIPVPDPRYAKSRNRIEYNSN
                     GYDCSNEKSLSWIEVSDGHFVYSSKSEINKYQQNLKVV"
     gene            complement(3988..5013)
                     /locus_tag="KPD58_002483"
     CDS             complement(3988..5013)
                     /locus_tag="KPD58_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0266244.1"
                     /translation="MEHLLEVNNLSVSFKVEEGEVQAVRNVSFNLKKGETLAIVGESG
                     CGKSVLCKSLMRILPYNGYIKNGEVLLKSSDLVKKSEKEMEDIRGKNISMIFQDPMTS
                     LNPTISIGKQIAEAVIIHQGISKSEAKKRAIELIELVGIDNPEKRFKQFPHHFSGGMR
                     QRIVIAIALACNPDVLIADEPTTALDVTIQAQIIDLIKDLQHKIGLSIIFITHDLGVV
                     ATIADRIAVMYAGKIVEIGTVEDIFYDPRHPYTWGLLGSLPTLDSQDDYLYNIPGMPP
                     NLLNPPKGDAFAIRNKNALKIDYEKEPPMFKINDTHSAATWLLHPDAPEVDVPVRVNC
                     GRVISNE"
     gene            complement(5088..6656)
                     /locus_tag="KPD58_002484"
     CDS             complement(5088..6656)
                     /locus_tag="KPD58_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0266245.1"
                     /translation="MKLKKLKVLSLVMILSLMAGCSSGGDKDKKADTPKDGKVLVYGS
                     NDYTSINPALYEHGEINSLIFNGLTAHDENNKVVPCLAKDWKFDEATNTYTFNLRDDV
                     KWHDGEKFTANDVKFTIETIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAM
                     LDYLTVGVLPKHALEGKDIATDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEP
                     GLDKVVFKIVPDDKAKAMQLKSGELDLAQITPKDMSNFEKDEKNFKVNIMKTADYRGI
                     LYNFNSKFFKDKKAKGLPNALSYAIDRKAIVDSVLLGHGVPAYSPLQMGPYNNPDIEK
                     FEYNPEKAKQEIEKLGWKLGSDGIYEKEGTKLAFEITAGESDQVRVDMAKICAQQLKE
                     IGVDAKAVVVTETDWANQDAHLIGWGSPFDPDDHTYKVFGTDKGANYSAYSNPTIDKI
                     LQKARETEDKDEKLKLYKQFQVEMTKDMPYTFIAYIDAIYVGKPNIKGLTPDTVLGHH
                     GVGIFWNIADWTIE"
     gene            complement(6769..7680)
                     /locus_tag="KPD58_002485"
     CDS             complement(6769..7680)
                     /locus_tag="KPD58_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0266246.1"
                     /translation="MKPQYSDFEIVGENYILVAEDEHIETKKTLSKKIKELPYISIII
                     LSIIVIGSVFSSFIMTHEPTYMDLASSNLAPNKTFLFGTDSMGRDIYSMIWYGGKISL
                     FIGLFSTVISTVIGIVYGSISGSASELVDDAMMRFTEIILSIPSILIIIFVQAILGNS
                     NPVSMSIVIGITSWMNISKIVRTEVRQIKNSEYILAAKSMGGGFFYVLKQHLLPNFVA
                     SIMFMVVTNIGAAIGTESTLSFLGIGLPIEIVSWGSMLSLSEEALLSNRWWIILIPGI
                     FLVTTLVCITNIGNYIRKNNNKKSSNL"
     gene            complement(7664..8650)
                     /locus_tag="KPD58_002486"
     CDS             complement(7664..8650)
                     /locus_tag="KPD58_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0266247.1"
                     /translation="MLKDILKKLLQFILVMFLLSFIVFYMARLAPGDPLISYYGDGVE
                     RMSTQEKENAMKKLGLDEPIYSQYIKWISNASKGEFGISFKYKQNVTSVINDVYINTI
                     ILGGSGYILTFVLALLLGIFCTLHEDKLIDRIICKLGTITNCIPSFWVALVLILIFSI
                     NLSILPSSGAYSMGEESSILSRISHLILPLTVLILSHLWYYTYMIRNKLLEEIREDYV
                     LLCKAKGLNNRTIVFKHCLRNIMPSYISIMAISIPHILGGTYVVEKVFSYPGLGTLCF
                     ESAKYHDYNMLLVLCLITGALVVFGNMLAQIINNKIDPRMKYDRGDTNEATI"
     gene            9010..9411
                     /locus_tag="KPD58_002487"
     CDS             9010..9411
                     /locus_tag="KPD58_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG0266248.1"
                     /translation="MLLKELYSKTMYTVEENFKENGLTHQQIIVIKLIAHNQELTISQ
                     LCDEMSLAKGTVSGIISRLEQIGYIEKFKKSNDKRNTYVKFTTTGFEFATNFKIKMQE
                     SFDDIFKNCDENELSDLVKNLRNILAKVKER"
     gene            9414..9812
                     /locus_tag="KPD58_002488"
     CDS             9414..9812
                     /locus_tag="KPD58_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03987 family protein"
                     /protein_id="HBG0266249.1"
                     /translation="MNYKLILAIVFITSALIFYTIGVFGERKAKILKKKHVIIFWLGF
                     IFDTLGTFTMSNIANSHTFEVKSALSQNLHSITGLLAIVLMLFHASWATFVLYKDDEE
                     KKKFFHKFSIVVWTIWLVPYFIGMFIGMAG"
BASE COUNT         3171 a         1686 c         1119 g         3970 t
ORIGIN      
        1 agactaaaaa aaattaccaa ataagttttt atactcattg gtaatctttt tcgattattt
       61 ctgcatattc taatattaat aatatttgat agcactcttt ttcattaatt tctatattat
      121 attttttatc taattgagat actatatctt ttattttgct tagactcttg tgataatcaa
      181 gtgttttttc tttatcaagc ttttcaattt ctctagtatt tccaatcaac ccatctacca
      241 tgaaagctaa atgcatcata agtccaatta ctttatcgta tttcttttct atattaaata
      301 ctaaaaacaa ttcatcagac acatatttta tacctttaaa tatcctatta taattagagc
      361 tttttaagga tgtctttaaa ccttcaacta catcatcata acctatactt atctttataa
      421 tattttttaa tttattgata ttttcatcct tatctatatc ttttttgcta atatagtcaa
      481 ttcctctaat attaattctt tgttcattga ttactgcaac aatattacta ctttctttaa
      541 gtttcgtaat atttaaactt aaatcttttt ctgttttata tgggacagat ataatatcta
      601 ttccatctaa gtacctactc atcttttctt ctaatatttt tcttgtttta ttatctacac
      661 cttctgaaac atgtgatgct ataataattg ttttagacag attttcttta ttttgaattt
      721 cattagttcc aaaatagtac tttctcgtgt tagttataga gttgtatacg tcatttaaag
      781 gtaatcccat attggccttc tgaactgctt ctaacacagt taatgtcgtc accataggaa
      841 ttactcttac tccacacttt ttttctttct ttataatttc atcaaaagag tttaatgaac
      901 ccatatctgt aaatagtaat acatctttat tgtctatatt attaattaaa tttttaaaat
      961 ctacaattac ttcatcatag cttttatcta acttcatatc gaaagaaata gcactgcact
     1021 ttccaataat atcaccaact gcttttacca tactacttgc tgtagtttct ccatgcatag
     1081 caactataat attaatgtta ttcactattt tattttgttc atcttgttta gcttgtacta
     1141 tgaataatgt aatatatgct acagtttcta tagataaaaa taaatcaaat tcattttcaa
     1201 ttattccaac tatttttaat gctgttttaa attcacttct gttatttctt ctaatttgat
     1261 tcaaatctat gttgtttgta gagctacttt tatccaatac gctttttata taaattgcca
     1321 ttgacttaaa tattgattca cttatgtcta tttcttcatc ttctttaata atttgtctta
     1381 ttgattcaca catgctttta aacttaacat tattaaatag agaagcatct gctatattca
     1441 tttctacaga tttattaaga ttagtattta ctgcattaat aatatttata tcattgtagt
     1501 taataacttc ttcttctcct aatgacgtaa atacaaaata ttctattcca tgtgttataa
     1561 cttcatctgc tttaatagac ttctctctat aatcatcact cacttcattt atagatttat
     1621 caaaatattt tttctctata taaactggtt ctttattatc tcttttatat cttagatatc
     1681 catttgcaac agctaatttt atatcatttt gaagttgacc tacattattt ttacaatcat
     1741 aaagtaatag tgatttaatt atttctttat gtacttttat tgaaatagaa atactttttg
     1801 attcttcctt gaaaaaattt ttgactaatt ccattctttc aatcattgtc ctatctctca
     1861 atgatggtat ttctattttc attggtattc ttctattgaa tgtatctaat agtgttgatg
     1921 tcggattttc tgtagtagca catattatta ataccttaga ctctctttct ttcgttgcat
     1981 ctcctagtct tttaaatgtt cccttatcta taaaagtaaa caacatttct tgaccttctg
     2041 ctggtagtct atgaatttca tctaaaaata gtatccctcc atttgcaatt tccaaaagac
     2101 cttttctgga agattctgca cctgtataag ctccttgttc taccccaaaa atatgtccca
     2161 taagtaattg tgcattatta gcataatcag cacaattaaa tgttacaaaa ggagctttat
     2221 tttttctaat tccactatct atagaataat cgtacatata tctagcaatc atactctttc
     2281 ctactcctgt ttctccatag ataatagtat gaagtccatt tgtaggatag agcattgcga
     2341 ccttgcattt ttctattata ggtcttaaac tatgttcttt acctattagt gtttcaaaac
     2401 tatctttttt tattttgttt tctacttttg ttcctttttt tatgccaagc aactcacttt
     2461 gtgtatcaag caattcattc ttgttaaggt taagatttgc agggatataa tacacaggtc
     2521 ttccttttat cttcgtaacc ataccctcat tgaataattg gtttaaatat gtacttatat
     2581 tgtttcttgc aattcctgtc ttttctgata attggttagc tgtaatccct ttttcatttt
     2641 ccctatatga tgattctaaa actttcatta ctttatcttt tttatatatc attacaagct
     2701 cccctacatt atagtatttt attaactaaa acccctatac acactttatt ttttaataaa
     2761 ctcttattct ttaactaagg ttataattta tcaaattttc cttactttaa attatagaaa
     2821 attattgtag aaaaaataag attattgctc attttttcta cattactatt tttaatttta
     2881 tagtactttc ttaacaacat actttacttg tttttaattt tattatattt gaatttatta
     2941 tatttgaaaa gtagaaataa aaaaccctat tttggagtag ttttaaaatt tctccaaaaa
     3001 tagggtttta gtttgtattt caaataaata cctgttcaag tatttttata ctaaacaact
     3061 tttaaatttt gttgatattt atttatttct gatttagaag aatatacaaa atgtccatct
     3121 gaaacctcaa tccaacttaa tgacttttca tttgaacaat catatccatt agaattatac
     3181 tctattctat ttcttgactt agcatatcta gggtcaggaa caggaattgc agaaagtagt
     3241 gattttgtat atggatgaat aggattatta tacaattcat ttgaatctgc caattctact
     3301 aattttccat tatacataac tcctatacgg tcactaatat gtctcaccat agataaatca
     3361 tgagctataa ataaacaagt taaattcttt tcttgttgca attttttaaa taaatttact
     3421 atttgtgctt gtatagacac atccaaagaa gctataggct catcagctat tataaattct
     3481 gggtctactg aaagagctcg tgctatgcca atacgctgtc tttgtccacc tgaaaaatca
     3541 gatggatact tatttgcata acttctatca agtcctacta gctttagcat ctcatatact
     3601 ttattcattc tatctgtttt atttttacag ataccttgaa tttttagtgg ctctgaaatt
     3661 atatctgcaa ttgtcatacg tggatttaat gatgaagtag aatcttgaaa aataatctgc
     3721 atacttttgt ttacatcttt ttttataaat ttgtacgttt ttttatctga tatgcaattt
     3781 cctttgtaaa ttacttctcc accagtagat tcatgtagtc ttattatagt tttaccagtt
     3841 gtagattttc ctgaacccga ctctcctacc aatccaaata tctcaccctt atatatatca
     3901 aaacttatat catcaactgc ttttacagta gtacttttgt ccaaatgaaa atattgcttt
     3961 aagtttttta tttccagtat tttttctcta ttcattagaa atcacccttc cacaatttac
     4021 tctaactggt acatctactt caggtgcatc tgggtgcaat aaccaagttg ctgcactatg
     4081 tgtatcatta attttaaaca ttggtggctc tttttcataa tctatcttaa gtgcattttt
     4141 attccttatt gcaaaagcat ctccttttgg tgggttcaat aaatttggag gcattccagg
     4201 tatattatat aaatagtcat cctgcgaatc taaagtaggt aatgaaccca acaaccccca
     4261 agtatatgga tgtctagggt cataaaatat atcctctaca gttcctattt ctacaatctt
     4321 tcctgcatac ataactgcta ttctatcagc tatagttgca actactccta aatcatgagt
     4381 tataaatatt attgacaaac ctattttatg ttgtaaatcc tttattaaat caattatttg
     4441 agcttgtata gttacatcta aagcagttgt aggttcatca gcaatcaata catctggatt
     4501 acaggcaagt gctatagcta ttactatacg ttgtctcatt ccacctgaaa aatgatgtgg
     4561 aaattgttta aatcttttct caggattatc aattccaacc aattcaataa gctctatggc
     4621 tctctttttt gcttcacttt tacttatacc ttgatgaatg attacagctt ctgcaatttg
     4681 ctttcctatt gatatagttg gattgagaga tgtcataggg tcttgaaata tcattgatat
     4741 attctttcct cttatatctt ccatctcttt ttcagacttc ttaaccaaat cacttgattt
     4801 aagtaaaacc tctccatttt taatatatcc attgtatggg agtatcctca tcaaactctt
     4861 acacagtaca gatttcccac aaccagattc tccaactata gccaatgtct cacctttttt
     4921 tagattaaaa gatacatttc ttacagcctg aacttctcct tcttctactt taaaacttac
     4981 agatagatta ttaacctcca ataaatgttc caaaaaccat caacccctat gctattcaaa
     5041 ccttagggat atatttttat ccctaaggtt tctaattaat ttcagtatta ttcaattgtc
     5101 cagtcagcta tgttccagaa tatacctaca ccatgatgac ctaaaactgt atcaggtgtt
     5161 aatcctttta tgtttggttt tccaacatat attgcatcta tataagcaat aaatgtataa
     5221 ggcatatcct tagtcatttc tacttggaat tgtttatata actttaattt ttcatcttta
     5281 tcttctgttt ctcttgcttt ttgaagtatt ttatctatag taggattaga gtaagcactg
     5341 taatttgcac ctttatctgt tccaaatact ttatatgtat ggtcatctgg gtcaaatgga
     5401 cttccccaac ctattaaatg agcatcttga ttagcccaat ctgtttcagt tacaacaaca
     5461 gctttagcat caacaccaat ttcttttaac tgttgtgcac atattttagc catatcaact
     5521 ctaacttggt cactctcacc tgctgttatc tcgaaagcta acttagtacc ttctttttca
     5581 tagattccat cagaacctaa tttccaacct agcttttcaa tttcttgttt agctttttct
     5641 ggattatatt caaacttttc tatatcagga ttattatatg gtcccatttg taaaggtgaa
     5701 tatgctggta caccatgtcc taataataca ctgtcaacta ttgcttttct atctattgca
     5761 tagcttaaag catttggtaa tcctttagct tttttatctt taaagaattt agaattaaaa
     5821 ttgtataata tacctctata gtcggctgtt ttcattatat ttactttaaa attcttttcg
     5881 tctttttcaa agttagacat atctttagga gttatttgag ctaggtctaa ttctcctgat
     5941 tttaattgca ttgccttagc cttatcatct ggaacaatct taaagactac tttatctaat
     6001 cctggttctt ttacaaaata atcactattc ttaacaagtg ttatactttg acctttgtcc
     6061 catttttcaa gtttaaatgg tccagtacct attggttttt gattaaattc atcagtagct
     6121 atatccttac cttccaaagc atgttttggt aatactccta ctgttaaata atcaagcatt
     6181 gctgtatttg gagcttttaa agtaattttt atagtattat cattaaccac atcaattttt
     6241 gttatatctt cataatttga tgctatctca gaagcattat ctggattcat tatagtttct
     6301 attgtaaact taacatcatt tgctgtaaat ttctctccat catgccactt aacatcatct
     6361 cttaaattaa aagtatatgt atttgttgcc tcatcaaatt tccagtcctt tgctagacaa
     6421 ggaaccacct tattattttc atcatgagct gttaatccat taaatattaa cgaatttatt
     6481 tctccatgtt cgtatagtgc aggatttata cttgtatagt cattacttcc ataaacaagc
     6541 acctttccat ctttaggcgt atctgccttc ttatctttgt ctcctccact agaacaacct
     6601 gccattagac taagtatcat tactaaactt aaaactttta acttctttaa tttcatgatg
     6661 ctcccccttt tgctttttat aaaatatttt tcaaagcttt gaccccattt atttaaatag
     6721 atatttcaaa tacctcagta tttgcttatt taatactcaa atgctgattt ataaattact
     6781 agatttttta ttattattct ttcttatata attacctata tttgttatac acaccaatgt
     6841 ggtaaccaaa aatatacccg gaatcagtat aatccaccat ctatttgaaa gtaatgcttc
     6901 ctctgacaaa gataacatac ttccccaaga aacaatttct attggtaaac caattcctaa
     6961 aaagctaagt gtcgattcag taccaatagc tgcgcctatg tttgttacaa ccatgaacat
     7021 tattgatgcc acaaaatttg gaagtaaatg ttgctttaat acatagaaga atcctcctcc
     7081 catgctctta gctgctagta tgtattcgct attttttatt tgtctaactt ctgttctcac
     7141 tatcttagat atattcatcc aacttgttat ccctattact atggacatag atactggatt
     7201 tgaatttcca agtatagctt gtacaaatat tataattaat attgaaggta tactaagtat
     7261 gatttctgta aacctcatca ttgcatcatc tacaagttca gatgctgaac cacttatact
     7321 accataaaca attcctataa cagttgatat tacagtggaa aatagtccta taaataaaga
     7381 tatttttcct ccataccata tcatagaata tatatctcta cccattgagt ctgttccaaa
     7441 taaaaaagtt ttatttggag ctaaattaga acttgctaaa tccatgtatg ttggttcatg
     7501 tgtcattata aaagatgaga atacacttcc aatgactatg attgataaaa taataattga
     7561 tatatatggt aattctttaa tttttttaga taaggttttc tttgtttcta tatgttcatc
     7621 ttctgctact aaaatataat tttctcctac aatttcaaaa tcactatatt gtggcttcat
     7681 tagtatcacc tctatcatat ttcattctag ggtctatttt attgtttata atttgagcta
     7741 acatattccc aaatactact aaagcccctg ttattaagca taaaactaat aacatattat
     7801 aatcatgata ctttgcactt tcaaagcaaa gtgttcctaa cccaggataa gaaaaaactt
     7861 tttcaactac ataagttcct cctagtatat gaggtatcga aatggccata atactaatat
     7921 atgatggcat tatatttctc aggcaatgct taaaaacaat agttctatta tttaaacctt
     7981 ttgctttaca taaaagcaca taatcttctc ttatttcctc tagtaattta tttcttatca
     8041 tatatgtgta ataccataga tggcttaata taagtactgt aagaggtaag attagatgtg
     8101 atatcctact taatatactg gactcttctc ccattgaata agcaccacta cttggaagta
     8161 tacttaagtt tatactaaat ataagtatga gtactaaggc aacccaaaat gatggaatac
     8221 agttggttat tgttcctaat ttgcatatga ttctatcaat gagcttatct tcatgtaatg
     8281 tacaaaatat tcctaacaat aaggctagca caaaggtaag tatatatcct gaaccaccta
     8341 atattatagt gtttatatag acatcattta taacagaagt aacattttgt ttatatttaa
     8401 atgaaattcc aaattcccct tttgatgcat ttgaaatcca ttttatatat tgactgtata
     8461 ttggttcatc caaaccaagt tttttcatag cattttcttt ttcttgtgta ctcattcttt
     8521 ctactccatc cccatagtaa gagattaatg ggtctcctgg agcaagtctt gccatgtaaa
     8581 aaacaataaa agataataaa aacattacta aaataaactg caacaacttt ttcagtatat
     8641 cctttaacaa accccaaccc ccctttgttg caataaaaaa aaccacataa aagcaaacgt
     8701 aaagtaacgt taaaaccttc gtggttccat tatcattaaa caaaattaaa ttttctatat
     8761 ttatctatga gttaatttta gcatcattta ttaactgtgt aaacaaactt tttaaaaatt
     8821 gttaaaattt catcaaatta ttttatttat acctaaaata aaatttttat attctattct
     8881 tcattatttt ctataaaatt ataaaatttt aggttgacta tataagaaaa gtatattata
     8941 ttagtttgta tgcaaaccat ttaagaggtg attttattga aaagtaatta tgactgtttt
     9001 agaatagcca tgctcttaaa agaactttat tctaaaacta tgtacacagt agaagaaaat
     9061 tttaaagaaa atggattaac acaccaacag attatagtta taaaactgat agctcataat
     9121 caagaactta caatatcaca actatgtgat gaaatgtctt tagctaaagg tacggtttca
     9181 ggcataatca gcagattaga acagattgga tatatagaaa aattcaagaa atctaatgat
     9241 aaaagaaata catacgttaa gtttacaaca acaggatttg aatttgccac aaattttaaa
     9301 attaaaatgc aagaaagttt tgatgatatt tttaaaaatt gtgatgaaaa cgaattgagt
     9361 gacttagtaa aaaatcttag aaatatattg gcaaaagtaa aggagagata attatgaatt
     9421 ataaattaat acttgcaata gtatttataa catcagcttt aattttctac actataggtg
     9481 tatttggaga aagaaaggca aaaattttaa aaaagaaaca tgtaattata ttttggcttg
     9541 gttttatatt tgatacttta ggaacattta caatgagtaa catagccaac agccatactt
     9601 ttgaagtaaa atctgcttta tcacaaaacc ttcatagtat tacaggtctt cttgcaattg
     9661 tgcttatgct ttttcatgca agttgggcta cttttgtcct atacaaagat gatgaggaaa
     9721 agaaaaaatt ctttcataag tttagtatag ttgtatggac tatatggtta gttccatatt
     9781 ttataggtat gtttattggt atggctggat agtaaaataa aaagtgtgaa agaaggatgt
     9841 aggtaaattt gtataagaca aatttaccta catccttctt tcacactttt acatatttat
     9901 ttataatact ccattatttc tttcatgttc cttaatacct atatag
//
LOCUS       DAELLV010000135         9863 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.135, whole genome shotgun sequence.
ACCESSION   DAELLV010000135 DAELLV010000000
VERSION     DAELLV010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9863
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.135"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            50..838
                     /locus_tag="KPD58_002489"
     CDS             50..838
                     /locus_tag="KPD58_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBG0266250.1"
                     /translation="MNQNEELEYKKNILKLALFVGELMIKNGAETSRVEDSVLRICRS
                     RGFYHVNIFTTPTVIIISDEKFDGFSFMKTIQSRGINLNKISLLNSFSREFVSDKEYD
                     IDNAIARLYEIQDVKPYPLWLFYSCTGIASACFACLLGGNYVLNFILTFIIAIFAAIT
                     YDKTMKISAISAFSCLVSSFLIALSGVLLVEIGILDDPKMLIVGAIMPLLPGVAFIKA
                     IRDLISGNLISGISRAFDAAMIIIAIASGVGFVLDTWYRIGGPF"
     gene            838..1311
                     /locus_tag="KPD58_002490"
     CDS             838..1311
                     /locus_tag="KPD58_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBG0266251.1"
                     /translation="MTDMSMILHFLFSGVATAGFAVFFNAPLYLLLPAGVTGGIGWIV
                     YVYLFNFTTNAVFAGFIAAALVSACSETLARKLKQPAIVFVIPGILPLIPGIGLYNTM
                     LSLIQKNYSLAMSKGTDALFLSAAIALGVLVVTSFVRTLNLLKIRKNFIPYRKSK"
     gene            complement(1403..1843)
                     /locus_tag="KPD58_002491"
     CDS             complement(1403..1843)
                     /locus_tag="KPD58_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HBG0266252.1"
                     /translation="MNQQGFIMEIVDDRTAKLKMQRHSACAACGKCATTNSAESKEIL
                     VEVDNTIGAKVGDHVEVSMDNMNVLKAAVMAYIIPLIALLVGTIGTYYILGAIGMTTG
                     VEAISGLVGICLTIICYLYLKKNDKKFRDSREFIPVITRILIDL"
     gene            complement(1858..2151)
                     /locus_tag="KPD58_002492"
     CDS             complement(1858..2151)
                     /locus_tag="KPD58_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HBG0266253.1"
                     /translation="MEERKLAPNNEREALIKRLKRIEGQVKGIQKMVEQERYCVDILV
                     QISAIRSAINRVGTIILENHIKGCVAESIKHDDADKTEEMIKELMDTINKFTK"
     gene            complement(2194..2595)
                     /locus_tag="KPD58_002493"
     CDS             complement(2194..2595)
                     /locus_tag="KPD58_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266254.1"
                     /translation="MKKCLYCNRDIKSPNNKLAIKYDDNMNIYPCRAECKEKIEEYIA
                     KKPTYKFINILEVLLGVGGIFCFLSKWTIAAQVVLGIDALVIFLFPLAGFKMNGKLSM
                     EQTKNQSRSIAISILAFIVIITVLYFKQVGK"
     gene            2828..4948
                     /locus_tag="KPD58_002494"
     CDS             2828..4948
                     /locus_tag="KPD58_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0266255.1"
                     /translation="MKQMELAIVSLKKDAGEIYENQIRQFLGDNLKINLYSFEEGNLK
                     FFKEKLILLSAYLKYDEIVKLSHYDAQIIVPKLTFEKNSIDMISKLEKDKIIYVYNLS
                     KDMAIETISLIHRLGIDNINILPCYPEIEFTPTDAVILTPGEKILPKFKNCEVIDLKY
                     RIIDLSCIVEIATKTKLKHLIKDDLIKKYVEKIIPTSYSTGELLLDANKFERQFDLLL
                     SIIDDGIICTNNDGIIQFYNHMARKILSINANEMIDSFVGDCIKDINFQSILTNKTPF
                     FEKLIKINHIDINLEIKHIQLNVFDGFILKMTKFSQLEKKQAKLRAQLVNSGNISKYT
                     FDDILGSSIQTINTKKIANKMAQSNSSILIIGESGTGKELFAQSIHSASRRKDGPFVA
                     VNCSTFQENLLQSELFGYDEGAFTGAKKGGKIGLFELANNGTIFLDEIGEMDLNSQSK
                     LLRVIQEKQVRRIGSNNVIDIDVRIIAATNRNLKELVSKNMFRRDLYFRLNVLPLKIH
                     PLRERPADIFEIFGSLKYDIPCNFILSEEVKEIFKMYRWEGNVRELRNLGEYFCYLEK
                     DIIEICDLPEYILDTIDSNYSRTVCNKVSDNIKKYQFNIGKDKNIMKYDYNFKRSLDE
                     YIFILDNLKKAYDLKERIGRKSLCKIALEENRFLTEQQIRNMLLELQDFGLVDILVGR
                     GGSIITSKGVEFLKNINRSNKLNS"
     gene            5173..6339
                     /locus_tag="KPD58_002495"
     CDS             5173..6339
                     /locus_tag="KPD58_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG0266256.1"
                     /translation="MNYNFNEIVDRSNNFSSKWSEMEKKYGTNDLLPMWVADMDFKAA
                     PCIIDSLKNRLEQEIYGYTTRPDSYNESIVNWLYRRHNWKIKSEWLIYSPGVIPAISL
                     LINELTKANDKIMIQEPVYSPFNSVVKNNNRELIINPLQKLENGNYIMDYEDIENKIK
                     DVKLFILCNPHNPVGRVWTKDELKKLGDICLKHNVKIISDEIHSDIILKKHKHIPMAS
                     ISKEFEKNTITCMAPTKTFNIAGLQSSYVILPDEKDYKLLDDAFTRIDIKRNNCFSLV
                     ATEASYNNGESWLESFLEYLESNIDFAIKYINENMPKLKVRKPEGTYLLWVDFSALGL
                     SDEELESILVQKGKVALNQGNSFGIGGSGYQRINLACPRSMLEEALIRIKNAIN"
     gene            6364..7806
                     /gene="nhaC"
                     /locus_tag="KPD58_002496"
     CDS             6364..7806
                     /gene="nhaC"
                     /locus_tag="KPD58_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBG0266257.1"
                     /translation="MKMNTERKIPSFRYSLLVMLAIIALTSVGIVVFNASITTMFLLS
                     WLIVVPAAMKLGYTNDEIEAFGFEVGKDAFQSNLIILSVGVLIAAWIAAGTIPTVVYS
                     GLTIITPKYFLLTTLIVCSLTSVATGTSWGTLGTSGIAMMSIGTSMGIPLGLTAGAVI
                     SGAFFGDKMSPLSDSTNLAAAVCKTDVITHMKHMFYTTGPAYVICIVLYTVIGFKYSN
                     NTIDYVQINQIKDVLNSNFHIGLVAMIPIIFLLLLLLLQKPPIISILSSAIMGLIIAV
                     FQEGEKIGDVLNYMLSGFTIDTGFVYADKLLNRGGIMSMAETVLLVFVVFVIAGILQK
                     TGFLEVLLQPLITKIGKSRTKLVGSTFIVSYFANAFSSSMMFTSVFVGTLMSPIYKEF
                     KLKPQNLSRIIEDTATLGGPLIPWNSNAVFCAQTLGVSPLEFIPYCFLSWITPIISFI
                     YGVTGFTMLTYTDDEIEELEIFDTAQRRVD"
     gene            7809..8078
                     /locus_tag="KPD58_002497"
     CDS             7809..8078
                     /locus_tag="KPD58_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266258.1"
                     /translation="MKFEYYYLIQDIAGILLAFIGLRMSIIGFRILSMKGLSINTLLI
                     VIKYCLFTIAGLNLLISKFGIRHWIWSVCMLIISIIINPRIKVSK"
     gene            complement(8155..9582)
                     /locus_tag="KPD58_002498"
     CDS             complement(8155..9582)
                     /locus_tag="KPD58_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp14"
                     /protein_id="HBG0266259.1"
                     /translation="MKLTKSISSMLCLSLVLSLSPVAYANEISRTTVENTLVGQDRYQ
                     TAVEVSKKGWSSANEAVVVNSESIVDALAVTPFAKLKNAPILLTEKNNLNSQTEKELK
                     RLGVKKVYIIGLSSSVSNDVQKQLESNNLTTDRVGGSDRYQTALSIAKKIEGLKDISE
                     IAVVNGYTGLADAVSIASVAATNGMVILPVSDASGISSFKDFIESKNINKSYIIGSTN
                     AVSDKIKQSLPNSERIGGADRNETNGKVIEKFYTSNKLNNVFVAKDGMKNQNQLIDAL
                     AVGVLASKENSPVLIVGEQLSATQKSVFSNKNATSITQVGSGGNENAFKELKDLQANS
                     NNKRVMYVTNTDKVNIRNDATIEASVIGYLNNGDEVEVLDVLKTGWVKIKYNEGIGYV
                     SGSYLTNNKPDNSNENIKIKYVKEKDGLNVRKGPSTEDEKMGQLSYGSKVETVEMFAT
                     GWVKIKYNGGYGYVSNDYLSDTPVI"
BASE COUNT         3446 a         1412 c         1385 g         3620 t
ORIGIN      
        1 aaatttgata taatttcaat attacgtttt ttaatttgga ggtgtattta tgaatcaaaa
       61 tgaggaactt gaatataaga aaaatattct aaaactagct ctatttgtag gggaactgat
      121 gataaaaaat ggagctgaaa catctagggt tgaagacagt gtccttagaa tatgcaggtc
      181 aagagggttt tatcatgtaa atatttttac aactcctaca gtaataatta tttcagatga
      241 gaaatttgat ggcttttcgt ttatgaaaac gattcaatca agaggtataa acttaaataa
      301 gatttcttta ttaaacagtt tttctcgaga atttgttagt gacaaagagt atgatattga
      361 taatgccatt gcacgtcttt atgaaatcca agatgtaaaa ccttatcctt tatggttatt
      421 ttactcttgt acaggaattg catctgcttg ttttgcttgt ttgcttggag ggaattatgt
      481 attaaatttt atacttacgt ttataatagc aatctttgct gcaattactt atgataaaac
      541 gatgaagata agtgcaatat ctgcattttc ctgtttagta tcttcttttt taattgcact
      601 ttcaggagtt ctactagttg aaataggcat tttagatgac cctaaaatgc ttatagtcgg
      661 cgctataatg ccactactcc caggagttgc ttttataaaa gctattagag atttaatatc
      721 agggaattta atatcaggaa tttctcgtgc ttttgatgct gctatgatta ttattgcaat
      781 tgcttctgga gttggttttg tccttgatac atggtataga ataggaggtc ctttctaatg
      841 actgatatgt ctatgatttt gcatttttta ttttcaggtg ttgcaactgc tggatttgct
      901 gttttcttta atgctccact atatctactt cttccagctg gagtaactgg cggaattggt
      961 tggattgtat atgtttattt atttaatttt actacaaatg cagtttttgc aggttttatc
     1021 gctgctgctt tggtctcagc gtgcagtgag actttagcaa gaaaactaaa gcaaccagct
     1081 attgtgttcg taattccagg tattcttcct ctaataccag gaattgggct atataataca
     1141 atgttgtctt tgattcagaa aaattattct ttggctatgt caaaaggtac agatgctcta
     1201 tttctaagtg ctgcaatagc acttggtgta ttagttgtaa cttcttttgt aagaacattg
     1261 aatcttttaa aaatcagaaa aaactttatt ccttacagaa aatcaaaata aaaatataaa
     1321 aatagcaatg tacttaaact ctacaaatac gtattgagta tttatttaaa aagtacattg
     1381 ctatttttta attataatta tcttaaaggt caattaatat tcttgttatt actggtataa
     1441 attctctact gtctctaaat tttttgtcat tcttctttaa gtataaataa cagattattg
     1501 taagacaaat tccaactaat ccacttatgg cttcaactcc agttgtcatt ccaattgccc
     1561 ccaatatata ataagtccct attgttccta caagaagtgc tataagaggt attatataag
     1621 ccattacagc tgcttttaat acattcatat tatccatact cacttctaca tggtcaccaa
     1681 cttttgcacc aattgtgttg tctacttcta ctaatatttc ttttgactct gctgaatttg
     1741 tagtagcaca ttttccacaa gcagcacaag ctgaatgtct ttgcattttt aactttgcag
     1801 ttctatcatc aacaatttcc ataataaatc cttgttgatt cataactaac cgcctcctta
     1861 ttttgtaaat ttatttatag tatccataag ctcttttatc atctcttcag ttttatcagc
     1921 atcatcatgt ttaatgcttt ctgctacaca tccttttata tgattctcta agataattgt
     1981 gcctactcta tttatagcag accttatagc agatatttga accaatatat caacacaata
     2041 acgttcttgt tccaccatct tttgtatacc cttaacttga ccttctattc tttttaatct
     2101 ttttatgaga gcttccctct cattgtttgg tgctaatttt ctttcttcca taaactcacc
     2161 tcatactcta atgatgtctt atacaattat gctctactta ccaacttgtt taaaatataa
     2221 tacagttatt attactataa atgccaaaat tgatattgca atagacctac tttgattctt
     2281 agtttgttcc atacttaact ttccattcat cttaaatcct gccaatggga ataaaaatat
     2341 aaccagtgca tcaattccaa gtacaacttg ggctgcaata gtccatttac tcaaaaagca
     2401 gaatattccg cccacaccta aaagaacctc taatatattt ataaacttat atgtaggttt
     2461 ttttgctata tattcttcaa ttttttcctt acattctgca cgacatggat atatattcat
     2521 attgtcatca tattttatag ctaatttatt atttggtgac tttatatccc tattacagta
     2581 aagacatttt ttcattttat attctcctct agtcttcctt aaaaaatttt gcctaaatta
     2641 ttatacgctc taatactatt ataccaaata gatacaaaaa acaaaaataa aaggaataat
     2701 tttagctact tcttttactt caatgtgtag tctataataa atttataata ataattaaat
     2761 aatattattt aatctaattt aattattatg taatctaaaa taagtcaaat ttgtatgaga
     2821 ggttaaaatg aaacaaatgg aattagctat tgtaagttta aaaaaagacg ctggagaaat
     2881 ttatgaaaat caaataagac aattcttagg tgataatcta aagataaatt tatactcatt
     2941 tgaagaggga aatttaaagt tttttaaaga aaaacttata ctcctatctg cttatttaaa
     3001 atatgatgaa atagtaaaat tatctcacta tgatgctcaa atcattgttc ccaagttgac
     3061 atttgaaaag aacagtattg atatgatatc caaattagaa aaagataaaa ttatttatgt
     3121 atataactta agtaaagata tggcaataga aacaatttct ttaatacata gattaggaat
     3181 agacaatata aatatcctgc cctgctatcc agagattgag tttactccca cagatgctgt
     3241 aatcctaact cctggcgaaa aaatcttgcc taagtttaaa aattgtgaag ttatagattt
     3301 aaaatacaga ataatagatt taagttgtat tgttgaaatt gctacaaaaa ccaaattaaa
     3361 acatctaata aaagatgacc taataaaaaa atacgtagaa aaaataattc ctacaagtta
     3421 tagtacaggt gagttattgt tagatgcaaa taagtttgaa agacaatttg atttgttact
     3481 atctataatt gatgatggaa ttatatgcac aaataacgat ggaataattc aattctataa
     3541 tcatatggct agaaaaattt tgtctataaa tgcaaatgaa atgattgact catttgttgg
     3601 tgattgcata aaagacatca attttcaaag catattgacc aataaaactc cattttttga
     3661 gaaattgatt aaaataaatc atattgatat aaatttagag attaaacata tacaattaaa
     3721 tgtatttgat ggatttatac taaagatgac aaagttttct caattagaaa aaaagcaagc
     3781 taaattaaga gcacaactag taaactctgg taatatcagt aaatacacat ttgatgatat
     3841 attaggttct agtatacaaa ctattaatac aaaaaaaatt gctaataaaa tggctcaatc
     3901 aaattcatct atacttataa ttggtgaaag tggtactggt aaagaattat ttgctcaatc
     3961 cattcatagt gcttctagga gaaaagatgg tccttttgtc gcagtaaatt gtagtacttt
     4021 tcaagaaaat ctactgcaaa gtgaattatt tggctatgat gaaggggctt ttactggtgc
     4081 taaaaaaggt gggaaaattg gtttatttga attagccaac aatggaacta tatttttaga
     4141 cgaaattgga gaaatggatt taaattctca atctaaatta cttagagtca tacaagaaaa
     4201 acaagtacga cgtattggtt ctaataatgt cattgacatt gatgtaagaa taattgcagc
     4261 tactaataga aatttaaaag aacttgtatc taaaaatatg tttagaagag atttatattt
     4321 taggttgaat gttcttcccc ttaaaataca tccacttagg gaaagacctg ctgatatatt
     4381 tgaaattttt ggttcattaa aatatgatat tccatgtaat tttattctaa gtgaggaagt
     4441 aaaagaaatc tttaaaatgt atagatggga aggaaatgta agagaattaa gaaacttagg
     4501 agaatacttc tgttacttag aaaaagatat tatagaaata tgtgatttac ctgaatatat
     4561 attggatact atagattcaa attatagtag aaccgtttgt aataaagtaa gtgataatat
     4621 aaaaaagtat caattcaaca ttggcaaaga caaaaacatc atgaaatacg actataattt
     4681 taaaaggtca ctagatgaat acatatttat attggataat ttaaaaaagg cttatgattt
     4741 aaaagaaaga ataggtagaa aaagcttatg taaaatagct ttagaggaaa atagattttt
     4801 gacagaacaa cagattagaa atatgctttt agaactacaa gactttgggc ttgtagacat
     4861 acttgttggt cgtggaggtt caattataac atcaaaagga gtagaatttc taaaaaatat
     4921 aaataggtct aacaagttaa acagttaaac ctatttaacc tatttaatta cattttcata
     4981 tatttaaaaa tttattagaa ataacctttt ccacaaagtt ttaagtataa ttaatatttt
     5041 tatcttatct atgctctgaa attacttaat attaaatatt aacttgtgaa taaatatttt
     5101 ttattatcaa tattggcacg ttgtttgctt aatattatta acataaaatt aaaattactg
     5161 gaggtattta caatgaatta taattttaat gagattgttg atagaagcaa taatttttca
     5221 tctaaatggt cagaaatgga aaaaaagtat ggaactaatg atttacttcc tatgtgggtg
     5281 gctgacatgg attttaaggc tgcaccttgt ataattgatt ccttaaaaaa tagactagaa
     5341 caagagattt atggctacac aacaagacca gattcttaca atgaatctat agtaaactgg
     5401 ctatatagga gacataactg gaaaataaaa agtgagtggt taatttatag tccaggtgtt
     5461 atcccagcta taagtctttt aataaatgaa ctcacaaaag caaatgataa aataatgata
     5521 caagaaccag tttacagccc ttttaatagt gttgtaaaaa ataacaatag agaactaatt
     5581 ataaacccat tacaaaaact agaaaatgga aactacataa tggattatga agacattgaa
     5641 aacaagatta aagatgtaaa attattcata ctttgtaatc ctcataatcc agttggtaga
     5701 gtatggacaa aagatgaatt aaaaaaacta ggagatattt gtcttaagca taatgtaaaa
     5761 atcatatctg atgaaataca tagtgacatt atattaaaaa aacataagca tattcctatg
     5821 gcatctattt ctaaagaatt tgaaaaaaat acaattactt gtatggctcc aactaaaact
     5881 tttaatatag ctggtcttca atcatcatat gtaatactac ctgatgaaaa agactataaa
     5941 ttgttagatg atgcctttac cagaattgat ataaagagaa ataattgctt tagcttagtt
     6001 gcaactgaag cttcatataa taatggtgaa tcttggctag aatcattttt agaatattta
     6061 gaatctaata tagattttgc tataaaatat ataaatgaaa atatgccaaa acttaaagtt
     6121 agaaaaccag aaggtactta tttattatgg gtagacttta gtgcacttgg attaagtgat
     6181 gaggaactag aaagtatact tgtacaaaaa ggtaaagttg ctctaaatca aggaaacagc
     6241 tttggcattg gaggaagtgg gtatcaaaga ataaacttag cttgtccaag aagtatgctt
     6301 gaagaagctc ttataagaat aaaaaatgca attaactaaa aatatttaag gagaatttgc
     6361 actatgaaaa tgaatacaga aagaaaaata ccttcattta gatattcctt gttagtaatg
     6421 cttgcaataa tagccctaac atcagttggt atagttgtat ttaatgcttc tataactaca
     6481 atgtttttac tttcatggct tatagtagtt cctgcagcca tgaaacttgg ttatactaat
     6541 gatgaaatag aagcttttgg atttgaagtt ggtaaagatg cctttcaatc aaacttaatt
     6601 atactttctg ttggtgtact aatagcagct tggatagctg ctggtacaat accaacagta
     6661 gtatatagtg gactaacaat aataacccct aaatattttc tgcttacaac attaatcgta
     6721 tgttcattaa cttctgttgc tactggtaca tcttggggga ctcttggaac ttctggtata
     6781 gcaatgatga gtattggtac tagtatgggc attcctcttg gtttaacagc tggtgcagtc
     6841 atttctggtg ctttctttgg agataagatg tcacctctat cagattctac aaacttagct
     6901 gcagcagttt gtaagacaga tgtaattaca catatgaagc atatgtttta taccacagga
     6961 cctgcatacg taatatgtat agttctgtat acagtaattg gatttaagta ctcaaataat
     7021 actatagact atgttcaaat aaatcaaatc aaggatgtat taaattcaaa ctttcatatt
     7081 ggtttagtag caatgattcc aataatattt ttattattac ttttattgct acaaaagcca
     7141 cccattatat ctatactaag ttcagctatc atgggactta ttatagcagt atttcaagaa
     7201 ggagaaaaaa taggtgatgt tttaaattac atgctatcag gatttactat agatactgga
     7261 tttgtctatg ccgacaaact attaaatcgt ggtggaataa tgagtatggc tgaaaccgtt
     7321 ttactagtat ttgtagtttt cgttattgct ggtattctcc aaaaaactgg attcttagaa
     7381 gtactgcttc aacctcttat aactaaaata ggaaaatcaa gaactaaatt ggttgggtct
     7441 acttttatag taagctactt tgcaaatgca tttagttcat caatgatgtt cacttctgta
     7501 tttgtcggaa ctctaatgtc tccaatatac aaagaattta aattaaaacc acaaaatcta
     7561 tctagaatta tagaagatac agctacactt ggtggtccat taataccatg gaattcaaat
     7621 gctgttttct gtgctcagac tcttggtgta agcccactag aatttattcc atattgtttc
     7681 ttaagttgga ttacacctat aatttcattt atttatggtg taactggttt tactatgctt
     7741 acatacacag atgatgaaat tgaagagtta gaaatatttg atactgctca gagaagagtt
     7801 gattgaatat gaaatttgaa tactattact taattcagga tattgcaggt atattattgg
     7861 catttatagg acttagaatg tctattattg gatttaggat actttctatg aaggggcttt
     7921 ctataaatac acttttaatt gtaattaaat actgcttatt taccatagca ggactgaatt
     7981 tattaatatc taagtttgga attagacact ggatttggag tgtttgtatg ttgataatat
     8041 ccataataat aaatcccagg attaaggtat caaaatagaa atagttatta gacttaaatt
     8101 tttataaaaa aactgcttta caaattaaag cagttttttt attgttactt ataattaaat
     8161 aactggtgta tcagataagt aatcattact tacataacca tatccaccat tatactttat
     8221 ttttacccat ccagtagcaa acatttctac tgtttctact ttgcttccat atgaaagttg
     8281 acccattttt tcatcttctg tacttggtcc tttcctaaca tttagaccat ctttttcttt
     8341 tacatacttt atttttatat tttcattact attatcaggc ttattatttg tcaaataaga
     8401 cccactaaca tatccaatac cttcattgta ttttattttt acccatccag tttttaacac
     8461 atctaaaact tctacttcat caccattatt tagatatcct ataacagaag cttctattgt
     8521 agcatcattt cttatattta ccttatctgt attagttaca tacattactc tcttattgtt
     8581 actatttgcc tgtaaatctt ttaattcttt aaatgcattt tcatttcctc cactaccaac
     8641 ttgagttata gaggtagcat ttttattcga aaatacactc ttttgtgttg cacttaattg
     8701 ttctcctacg attagtacag gagaattttc ttttgatgct agtactccta ctgctagagc
     8761 gtctataagt tgattttgat ttttcattcc atccttagcc acaaatacat tgtttaattt
     8821 attagatgta taaaactttt ctattacttt accattagtt tcatttctat ctgctccacc
     8881 tattctttct gaattaggta aactttgttt tattttatct gatacagcat tagtagagcc
     8941 tattatgtat gatttattta tattcttaga ttctataaaa tctttaaagc tagatatacc
     9001 agatgcatca gatactggaa gtataaccat tccattagta gcagctacag aagctatact
     9061 aactgcatct gcaagacctg tgtacccatt tactacagct atttctgata tatcttttaa
     9121 tccttctatt tttttagcta tactcaaagc tgtttggtat ctatcactac caccaactct
     9181 atcagtagtt aaattattag attctaactg tttttgcaca tcatttgata cagaactaga
     9241 tagaccaatt atataaactt ttttaactcc taatcttttt aattctttct cagtttgtga
     9301 atttagatta tttttttctg tcaataatat aggagcattt tttaatttag caaatggtgt
     9361 tacagctaat gcatctacaa tagattcact atttacaact acagcctcat ttgcactact
     9421 ccatcctttt ttacttactt caacagcagt ttgatatcta tcctgaccta ctaaagtatt
     9481 ctcaactgtt gttctactta tttcatttgc atatgctact ggtgataagc ttaaaactag
     9541 agataagcac agcattgatg atattgattt agttaatttc atcttgactc cccctaaaat
     9601 tatagtatct aaatgtttcc tatttaaaat agtataacat aataatttaa gtaacatcat
     9661 tattataaaa tattattttt cttatctaat aagcttttat tcttacttga cattaatact
     9721 ttgctcatca aatgaatttt ttatataact aaagtttgta acaatatttc cttagttaat
     9781 catttaaagt tacttttttt aattagatta tgtacaaaat ataaataaca atttaaataa
     9841 ctttttaata tattgtagtt tat
//
LOCUS       DAELLV010000136         9854 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.136, whole genome shotgun sequence.
ACCESSION   DAELLV010000136 DAELLV010000000
VERSION     DAELLV010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9854
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.136"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            335..913
                     /locus_tag="KPD58_002499"
     CDS             335..913
                     /locus_tag="KPD58_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG0266260.1"
                     /translation="MGITLSKGQKVDLTKGNPGLKNILVGLGWDTNKYDGGFDFDLDT
                     AAFLTGASGSVTNDGDFVFYNNLKHTSGAVEHLGDNRTGEGDGDDEQIVVDLSKIPGE
                     ISKISFTVTIHDATERRQNFGQVSNSYIRIVDKDTNEELIKYELGEDFSIETAIVVAE
                     IYKHNGEWKFNALGSGFEGGLAALCGNFGINL"
     gene            952..1530
                     /locus_tag="KPD58_002500"
     CDS             952..1530
                     /locus_tag="KPD58_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG0266261.1"
                     /translation="MAIVLKKGQKIDLTKGNPGLKNIKLGLGWDTNSFDSGYDYDLDV
                     SVFMVGESQRVEKDEDFIFYNNLKHPSGAVEHLGDNRTGEGDGDDEEILVNLETMPKH
                     IQKIAVTVTIYEAAERRQNFGQVSNSYIRVLNSENDEEILRYDLGEEFSIETAITVCE
                     IYKYNGEWKFSAVGAGFEGGLEALCRNYGLNV"
     gene            1616..2245
                     /locus_tag="KPD58_002501"
     CDS             1616..2245
                     /locus_tag="KPD58_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG0266262.1"
                     /translation="MSINLSKGDKIDLKKSNPGLSNILVGLGWDPVQQSGGGFFKSLF
                     GGGQADIDCDASVFMLNQEGKLSGIKDLIYFGNLKSACKSVLHTGDNLTGEGTGDDEQ
                     ILVNLDKVPSNIHKLLFVVNIYNCVDRKQHFGMIENAYIRVEDQGNKKEIAKYNLSDN
                     YSEKTTLIVGAIYRKDGSWQFKAIGEGTKDAGLKEVMQNLDRIECAYGI"
     gene            2235..4994
                     /locus_tag="KPD58_002502"
     CDS             2235..4994
                     /locus_tag="KPD58_002502"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HBG0266263.1"
                     /translation="MEFNNDIKYYNVDKEEVTKDLSVNPEKGLSESEVKTRREKYGLN
                     EFTPKEEGSFWDDLKESLSEPMIVILIIAAVVSAVIGETHDAIGIVGAIAIGIAIGMI
                     TEGRSKKAAEALSKLTENIEVKVLRDGEVHQISKSELVPGDIVYIETGDMVPADGRLI
                     ESINLKIREDMLTGESDDVSKKCDVVVSMEEIESKGAVVVQEPIPAKQINMVFGGTLV
                     AYGRGALVVTSIGDNSEMGKIAQNLSETDEQTPLQIKLGNLGGLIAKVSSAVAGLLFI
                     FMVFQMISKNVLNVDMSGILPFLESIDPVKTAFTVCVALIVAAVPEGLPTMINMTLAI
                     TMQKMAKINALVTKKEACETIGSVSVICSDKTGTLTENRMTVEVAYVDGRYIESSEEE
                     INSYFEENCMINSTADVEHNDGDIKYLGSATECALLLYYKNVDYRQVRKNSNIVAQNP
                     FTSDSKRMTSVIGQDNHHVLLSKGAPEVLLELCSHIQRGKDIVPLTENIKHEILEEIK
                     KLQIKSMRTLGFAYKEISQAEEEAAVTAESDAMNVSAMENNLVFSGFVGIRDPLRKDV
                     IESVNTANKAGVSVKMLTGDNINTARAIGEELGLLKNNMRVVEASYIDTLDDEELKQE
                     IQSISIVARSKPDSKMRIVSALQKSGDVVAVTGDGINDAPALSKADVGIAMGISGTEV
                     SKNAADIILTDDSFSTIVKGIKWGRGIYDNFQRFVQFQLTVNVIAFLVAIISQVMGQE
                     MPFTTIQLLWVNIIMDGPPALALGLEPVRDHVLNRKPVDRKANIISKSMVYTIVLNAF
                     YITAILMLQSTFNFLGVDEAHKGTVMFALFAFSALFNAFNCREFNADSIFPNFTHNKL
                     ALQIIVLTGIAQVIFTQLFQDFFNSVAMDFMTWVKVLCVAASVIVVNEVVKCIIRLVK
                     LKKTA"
     gene            5081..6643
                     /locus_tag="KPD58_002503"
     CDS             5081..6643
                     /locus_tag="KPD58_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceG family protein"
                     /protein_id="HBG0266264.1"
                     /translation="MGDSKVFEKIFSSQSDRGNYTPSKGYLSYFISYIGLEDEVLYNL
                     EIFKTKQNIDSKKDIALFTDVIANPSDFDIINYFKSGLQKYRTSMEDVDINILGFEEI
                     DYKIKQAMDRVLKEEEKEFTNDRVKQNFIVKIMAWIKIYIGALDINKNEAPKVIFYGD
                     IKKHEVYLLLILYLAGFDVLYLNPNSKSNIDILKSERYNIEFEEANIIEEKISFEERV
                     ILGEKIDKSSVKKAFTVGAEASKRISEELLNDAGFIKPWQLQDRKIKNLLLSSTVDEI
                     SIYWNQPLKLRPGFKFNDAIVEAPNFLSKINGIYNDKNEYIKFLDLLRDSESSTFIEF
                     NGDVDRFSKAFTREAFSLSFLLDSKGAIDKNSVLNNKDYSISTLALNQQIMILEKVEE
                     LLEGSMFLNGLSGEDKIKGLFTVLHMDKKFVHMMNNFDYSLINPKLIIYMYKSIVFDK
                     EIVFLMLLLSKIGFDIIILCPGGENNIENVINNQLIDVHRLDKMVYDLKLNSLENDIP
                     LLKKIFGKRRRF"
     gene            6644..7780
                     /locus_tag="KPD58_002504"
     CDS             6644..7780
                     /locus_tag="KPD58_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBG0266265.1"
                     /translation="MGININSFSKDKNSVEVTENLPTAVQEENFDIMEYTNNKKNELR
                     KSKEVEALTSLIEVENPDTILQFGRKASEGVARVSDSLLNTIKLNRNEENSKMLVHLT
                     KIMDKFDLDDFQETKEPNFVQKLFKKANNAIEMMFQKYETLGGEVEKIQIELEQYERD
                     IALSNKQIGAMLNENFEFYNELQKYIVAGEMAIEEMDNEILPFFKQKSETSGDQMDVV
                     NYQELLKVYDMLNQRVYDLRIAENIAIQTIPMLRGMQHNNYGLIRKINSAFVVTLPVF
                     KQCLSQAILLKKQELQAKSLKALDDKTNELLLRNAQNVSTQSAQIARMAGTSSVQIET
                     LEKMYNTIKSGIDETMRIEENNRALIKDNTKRLEELNTTIIYNK"
     gene            7844..8920
                     /locus_tag="KPD58_002505"
     CDS             7844..8920
                     /locus_tag="KPD58_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HBG0266266.1"
                     /translation="MKYFDYICKDDLERIFLKEPEDFSAKTEKDVLKYALGAFLYVPA
                     TQYNMIYKSIIGDVKGVRPLAICLEDAVGVNGELEAIENLRLILKNISNESITNKDGI
                     PLIFVRIKDVEQLLRIKEIIIKNSHFITGILIPKANSELIENCIEALDFMNLQDMYVI
                     PIVETKEFIYNEKKELSFTNLYNAILRHKSRILSIRVGITDILGMYGIRRDKNFSIYN
                     NLICSSFILDIITYLQRPELDIPISGGVSEFFDMTNKDIRNKYIEEILLDKYHGLIGK
                     TVIHPMQIQIVQALSTVSYEDFTDALDILDSTDSKYGVSKGVLGERMNETNPHFLWAK
                     KTLILSKIYGVLNKGVDYEELLKF"
     gene            8898..>9854
                     /locus_tag="KPD58_002506"
     CDS             8898..>9854
                     /locus_tag="KPD58_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HBG0266267.1"
                     /translation="MKNCLNFNCEIDIIDNPLNLELNDFLDVGIRNNSKRRFLFISKK
                     LGKHLACKPSEMDNLGKLMATIYNEKNKEESNGTVIAFAETGTAVGHSFFDYLCGDYE
                     FIHTTREQFDELENLEFLEEHSHATDQNLYFGMLNNFKFGDEIILVDDEITTANTCMN
                     IIRKIQSIYPKKKYTICSILNWVSDENLSKVTRLERELDCKIDFVYLFKGNFKFYIDE
                     DIISNYNDNYSITKLETISSDKVNLVVNHIYVDLEDYIGNKKYVKYTGRFGINRREQF
                     RLKEIVKRESKKITMASKESLKNEKEKILFLGTEEFMYIPMLF"
BASE COUNT         3897 a         1015 c         1882 g         3060 t
ORIGIN      
        1 ggtagtagtg gcgaagacac cgtccgaagt agcttggctt tgtaaccacc tctgttgtac
       61 tgtttaagta taaataagtg tgttagccac caagaatccc cttgctttag ctatggggag
      121 tgtgtcaaat attaaacata gcttttatac atttaataag gtatgtaaaa tctatgtaaa
      181 aagtaagaaa tataaacatt tttttaatta tattttaaaa atatcaagca agttttcttc
      241 acaaatgaat aaaaaatgtt ataatatgat taaagaaaaa gataaataat tagcaagatt
      301 tgtttagcta aaattaacaa gggagcgata aattatgggt ataacattat caaaaggcca
      361 aaaggttgat ttaactaaag gaaatccagg tcttaaaaat attttagtag gtttaggatg
      421 ggatactaac aagtatgatg gtggatttga ctttgattta gatacagcag catttttaac
      481 aggagcaagt ggaagtgtta ctaatgatgg agattttgta ttttacaaca atttaaaaca
      541 tacatctgga gcagttgagc atctaggaga taacagaact ggcgaaggtg atggagatga
      601 tgaacaaata gttgttgatt tatctaagat tcctggagaa ataagcaaga tttcttttac
      661 tgttacaatt catgatgcaa cagaaagaag acaaaatttt ggacaagtaa gtaattctta
      721 tataagaata gtagataaag acacaaatga agagttaata aagtatgaat taggagaaga
      781 ttttagtata gaaacagcta tagtagtagc tgaaatatat aagcacaatg gagaatggaa
      841 gtttaatgca ctaggttctg gctttgaagg tggtcttgct gcattatgtg gaaactttgg
      901 aataaactta taatttcaac tataacaata aactaaaggg ggatattttc catggctata
      961 gtattaaaaa aaggacaaaa aatcgattta actaagggaa atccaggtct taaaaatata
     1021 aaacttggac taggatggga tacaaattca tttgatagtg gctatgatta tgatttagat
     1081 gtaagtgttt tcatggttgg agaatctcaa agagttgaaa aagatgaaga ttttatattc
     1141 tacaataact taaaacatcc atctggagca gttgaacact taggtgataa tagaactggt
     1201 gaaggtgatg gagatgatga ggaaatatta gtgaatttag agacaatgcc aaagcatata
     1261 caaaagatag ctgtaacagt tacaatatat gaggctgctg aaagaagaca aaattttgga
     1321 caagtaagta actcatatat aagagtatta aattctgaaa atgatgaaga aatattaaga
     1381 tatgacttag gtgaagaatt tagtatagaa actgcaatta cagtatgtga gatatataag
     1441 tataatggag aatggaagtt tagtgcagtt ggagcaggat ttgaaggtgg cttagaagca
     1501 ctttgtagaa actatggctt aaatgtttaa tttaagcaat ataatatatt aaatataaat
     1561 aacctataca aataatgtat aggttatatt atataaaatt ttgtggaggt ttagtatgtc
     1621 gattaattta tcaaaaggtg ataaaataga tttaaaaaaa tctaacccag gactgagtaa
     1681 catacttgta ggtctaggat gggaccctgt gcaacaatct ggtggaggtt tttttaaatc
     1741 gttatttggt ggaggacaag ctgatataga ttgtgatgca tctgtattta tgcttaatca
     1801 agaaggtaaa ttaagtggaa taaaagattt aatttatttt ggaaatctaa aaagtgcctg
     1861 taaatcagtt ttgcatacag gagacaatct aactggtgag ggtacaggtg atgatgaaca
     1921 aatcctagtt aatttggata aagttccatc aaatattcat aaattattgt ttgtagtaaa
     1981 tatatacaat tgtgtagata gaaaacaaca ctttggaatg atagaaaatg cctatataag
     2041 agttgaagac caaggtaata agaaagagat agcaaaatac aatctatctg ataattattc
     2101 tgaaaaaact actcttatag taggagctat atatagaaaa gatggaagtt ggcaatttaa
     2161 agcaattggt gaaggaacaa aagatgcggg tcttaaagaa gtaatgcaaa atttagacag
     2221 gattgagtgt gcatatggaa tttaataatg atatcaaata ttataatgta gataaagaag
     2281 aagttacaaa agacttatct gttaatcctg aaaaaggttt atcagaatca gaagtcaaga
     2341 ctagaagaga aaaatatggt ttgaatgaat ttactccaaa agaagagggt agtttttggg
     2401 atgatttaaa ggaaagttta agtgaaccaa tgatagttat cttgattatt gcagcagtag
     2461 taagtgcagt aattggggaa actcatgatg caatagggat tgtaggtgct atagcaatag
     2521 gtatagcaat aggtatgatt acagaaggtc gttctaagaa agcagcagag gctctttcta
     2581 agttgactga aaacatagaa gtaaaagtat tgagagatgg ggaagtacat caaatttcta
     2641 agagtgaatt ggtaccaggt gatatagtat atattgaaac tggtgacatg gtacctgctg
     2701 atggtagact tatagaaagt ataaacctta aaattagaga agatatgtta acaggagaat
     2761 ctgatgacgt atctaaaaag tgtgatgtag tagtttctat ggaagagata gaatctaaag
     2821 gagcagtagt agtacaggag ccaatacctg caaaacagat aaatatggtg tttggtggca
     2881 cacttgtagc ttatggtaga ggagctctag ttgtaacttc aattggagat aatagtgaaa
     2941 tgggtaaaat tgctcaaaac ttatcagaaa cagatgagca aactccactt cagataaaac
     3001 ttggaaattt aggtggtttg atagccaaag tttctagtgc agtagcagga ttactattta
     3061 tatttatggt tttccaaatg ataagtaaga atgtattaaa tgtagatatg tctggaatat
     3121 taccgttttt agaatcaata gacccagtta aaacagcatt tacagtatgt gttgcgttga
     3181 tagttgcagc agtgccagaa ggtcttccaa ctatgataaa tatgacactt gcaataacaa
     3241 tgcaaaaaat ggctaaaata aatgcattgg ttactaaaaa agaagcttgt gaaacgatag
     3301 gttctgtttc tgtaatatgt tcagataaaa ctggtacatt aactgaaaat agaatgactg
     3361 tagaagttgc atatgttgat ggcagatata ttgaaagttc agaagaggaa ataaatagtt
     3421 attttgaaga aaactgtatg ataaactcta cagctgatgt agagcacaat gatggtgata
     3481 ttaagtattt aggaagtgct actgaatgtg cattgctttt atattacaaa aacgtagatt
     3541 atagacaagt tagaaaaaat tctaatatag tagctcaaaa tccttttact tcagattcaa
     3601 agagaatgac ttctgttata ggtcaagata atcatcatgt actattatca aaaggagcac
     3661 cagaagtctt acttgaatta tgttcacata ttcaaagagg aaaagacata gttccattaa
     3721 ctgaaaatat aaaacatgag attttagaag aaataaagaa attgcaaata aaatcaatga
     3781 gaactttagg gtttgcatat aaagaaatct cacaagctga agaagaagca gcagtaactg
     3841 ctgaaagtga tgctatgaat gtaagtgcaa tggaaaacaa tttagtattt agtggttttg
     3901 ttggtataag agacccactt agaaaagatg ttatagagtc tgtaaataca gcaaataaag
     3961 ctggtgtttc tgtaaaaatg cttacaggag ataatataaa tacggcaaga gctataggtg
     4021 aagagttagg cctattaaaa aataatatga gagtggttga agcttcatat atagatactt
     4081 tagatgatga agaattaaaa caagagatac aaagtatttc aatcgtggca agaagtaaac
     4141 cagatagtaa aatgagaata gtttcagctc tgcaaaagag cggagatgtt gttgcagtaa
     4201 caggtgatgg tataaatgat gctccagcac tttcaaaagc agatgttggt atagcaatgg
     4261 gtatatctgg tacagaggta tctaaaaacg cagcagacat aattttaact gatgatagtt
     4321 ttagtaccat tgtaaaaggt attaaatggg gtagaggaat ttatgataac ttccaaagat
     4381 tcgttcaatt ccaattaact gtaaatgtaa ttgcattttt agtggctata atatctcaag
     4441 ttatgggaca agagatgcca tttactacaa tacaattatt atgggtaaac atcattatgg
     4501 atggaccacc agcactagca ttaggtttag agccagttag ggaccatgtg ttaaatagaa
     4561 aacctgttga tagaaaagcc aatataattt ctaagtcaat ggtgtataca atagtattaa
     4621 atgcatttta tataacagca atactaatgt tacaatctac atttaatttc ttaggagttg
     4681 atgaagcgca taagggaaca gtaatgtttg cgttatttgc ttttagtgca ctgtttaatg
     4741 catttaactg tagagagttt aatgcagata gtatcttccc taattttaca cataacaaat
     4801 tagcactaca aataatagtg ttgacaggaa ttgcacaagt aatatttact cagctattcc
     4861 aagatttctt taactctgta gcaatggatt ttatgacatg ggtaaaagtt ttatgtgtag
     4921 cagcaagtgt aattgtggtt aatgaagttg taaaatgtat aataagactg gtaaaactaa
     4981 aaaagactgc ttaaaacaaa ttttttagaa gaaaacagac tgactcaaaa tatgaatttt
     5041 gggtcagttc tgaataaaca tttaaattgg gaggatagtt atgggtgact caaaggtatt
     5101 tgaaaaaata ttctccagcc aatcagatag aggaaattac actccttcaa agggatatct
     5161 aagttatttt atttcgtata ttggtttaga agatgaagtt ttgtacaatc tcgagatatt
     5221 taagacaaaa caaaatattg atagcaaaaa agatattgct ttatttactg atgttatagc
     5281 aaatcctagt gattttgata ttataaatta ttttaaaagt ggacttcaaa aatatagaac
     5341 tagcatggaa gatgtagata taaatatttt gggatttgaa gagattgatt ataaaataaa
     5401 gcaagcaatg gatagagtgt taaaagaaga ggaaaaagaa tttactaatg atagagttaa
     5461 acaaaatttt atagtgaaaa taatggcatg gataaaaatt tatatcggtg ctttagatat
     5521 aaataaaaat gaagctccaa aagtaatatt ttatggagat ataaaaaaac atgaagttta
     5581 tttactctta atattatact tggcaggttt tgatgtttta tatcttaatc caaattcaaa
     5641 gagtaatata gatattttaa aatcagaaag atacaatata gagtttgaag aagccaacat
     5701 aatagaagaa aaaatttctt ttgaagaaag ggttatttta ggggaaaaaa tagataaaag
     5761 ttctgtaaaa aaagctttta ctgttggggc agaagcatca aagcgtataa gtgaggaatt
     5821 gttaaatgat gcaggattta taaaaccttg gcaactccaa gatagaaaaa taaaaaatct
     5881 acttctaagt tcaactgttg atgaaatatc aatatactgg aatcaaccac ttaaattaag
     5941 accaggattt aagtttaatg atgctattgt agaagctcca aattttcttt ctaagattaa
     6001 tggaatatat aacgataaga atgaatatat taagttttta gatttattaa gagattcgga
     6061 aagttcaaca tttattgaat ttaatggaga tgtagataga ttttcaaaag cttttacaag
     6121 agaagctttt agtttatcct ttttattgga ttcaaaaggt gcaatagata aaaactctgt
     6181 tttaaataat aaagattatt ccatatcaac actagcattg aatcaacaaa ttatgatttt
     6241 agagaaagtg gaagagttgt tagaaggaag tatgtttttg aatggtttaa gtggagaaga
     6301 caagataaaa ggtctattta cagtacttca catggataag aaatttgttc atatgatgaa
     6361 caatttcgat tactcactta taaacccaaa attgattata tacatgtaca agagtattgt
     6421 atttgataaa gaaatagttt ttcttatgct tttacttagt aagattggat ttgatattat
     6481 catattatgt ccaggaggag aaaacaatat agagaatgtt ataaataacc aattgattga
     6541 tgtacacaga ttggataaaa tggtatatga ccttaaatta aattcgcttg aaaatgatat
     6601 acctttactt aaaaaaattt ttggaaagag gagacgattt tagatgggga taaatataaa
     6661 tagtttttca aaagataaga actctgtaga ggttactgaa aatttgccta cagcagttca
     6721 agaagaaaac ttcgatataa tggaatatac aaataataaa aaaaatgaac tgagaaagtc
     6781 taaggaagtt gaggcattga catctcttat agaagtagaa aatccagata cgattttaca
     6841 gtttggtagg aaagcatctg aaggagtagc aagagtttct gatagccttt taaatacaat
     6901 aaaattaaat agaaatgaag aaaattctaa aatgttggtt catttaacta aaataatgga
     6961 caagtttgat ttagacgatt ttcaagaaac taaggaacca aatttcgttc aaaaactttt
     7021 caaaaaagct aataatgcca ttgaaatgat gtttcaaaag tatgaaacat tgggaggaga
     7081 agttgagaaa attcaaattg agcttgaaca atacgaaaga gatatagcac tttctaataa
     7141 gcaaataggt gctatgttaa atgaaaactt tgagttttac aatgaacttc aaaaatatat
     7201 tgttgctgga gaaatggcaa tagaagaaat ggataatgaa atactacctt tctttaaaca
     7261 aaaatcagag actagtggag accaaatgga tgttgtcaat tatcaagaac tattaaaagt
     7321 ttatgatatg ttaaatcaaa gagtctatga tttaaggata gcagaaaata tagctatcca
     7381 aacaatacct atgcttagag gtatgcaaca taataattat ggtttaatta gaaaaataaa
     7441 tagtgcattt gtcgtaactt taccagtatt taaacaatgt ttatctcaag ctatattact
     7501 taaaaagcaa gaattacaag caaaatctct aaaagcactt gatgacaaaa ctaatgaatt
     7561 attgcttaga aatgcacaaa atgtaagtac tcaaagtgca caaatagcta gaatggctgg
     7621 aacaagttct gttcaaatag aaacattgga aaaaatgtac aatactatca agtctggtat
     7681 agatgaaact atgagaatag aagaaaacaa tagagcttta attaaggata atacaaagag
     7741 attggaagaa ttaaatacga ctataattta taataagtag tattatatta agggctaatt
     7801 tgtataaagt tggccctttc tatgtatatg aggaggctag acattgaaat atttcgatta
     7861 tatctgcaaa gatgatttgg agagaatttt tttgaaggag ccagaagatt ttagtgctaa
     7921 gactgaaaaa gatgtgttga aatatgcact gggcgctttt ttatacgttc cagctactca
     7981 atataatatg atttataaga gtattatagg ggatgttaag ggagttagac cattagctat
     8041 ttgtttagaa gatgctgttg gagtaaatgg agagttggag gctatagaaa atcttagatt
     8101 aattttaaaa aatattagca atgaaagtat aactaataaa gatggaatac cactaatttt
     8161 tgtaagaata aaagatgtgg agcaactgtt gagaattaaa gagattataa ttaaaaatag
     8221 tcattttata acaggaatat taattcctaa agcaaattcg gagttaattg aaaattgtat
     8281 agaagcttta gattttatga atcttcaaga tatgtatgtt attccgatag tagagacaaa
     8341 agaatttata tataatgaaa aaaaagaact tagttttact aatttgtaca atgctatttt
     8401 aagacataag tcaagaatat taagcattag agtaggcata actgatatat tagggatgta
     8461 tggtataagg agagataaaa acttttctat atataataat ttgatttgtt cttcatttat
     8521 attagatata atcacatatc ttcaaagacc agagttagat atacctataa gtggtggagt
     8581 atcagaattt tttgatatga cgaataagga tataagaaat aagtatatag aagaaatatt
     8641 attggataaa tatcatggat taattggaaa gaccgtaatt catccaatgc aaattcaaat
     8701 tgtacaagcg ctatctacag tatcatatga agattttact gatgctttgg atatactgga
     8761 tagtacagac agtaagtatg gtgtgagcaa gggtgtatta ggagagagaa tgaatgaaac
     8821 aaatccacat tttttatggg ctaaaaaaac attgattcta tctaaaatat atggtgtatt
     8881 aaataaagga gtagactatg aagaattgct taaattttaa ttgtgagatt gacattattg
     8941 ataatccatt gaatttagaa ctaaatgatt ttttagacgt aggaataaga aataatagca
     9001 aaagacgttt tttgtttatc tcaaagaaac tcggtaaaca tttagcatgt aagccaagtg
     9061 agatggataa tttgggaaaa ttaatggcta caatttataa tgaaaaaaat aaggaagaat
     9121 caaatggtac tgttatagct tttgctgaaa ctggtacagc tgttgggcat agtttttttg
     9181 attacttatg tggagattat gaatttattc atactactag agagcagttt gacgagttag
     9241 aaaatctgga atttttagag gaacattccc atgcaacaga ccagaattta tactttggta
     9301 tgcttaacaa ctttaaattt ggagatgaga taatattggt tgatgatgag attaccacag
     9361 ccaatacatg catgaatata attaggaaaa ttcaaagcat ttatcctaaa aagaaataca
     9421 ctatatgctc aatactcaat tgggtaagtg atgaaaattt aagtaaagtt actcgtttag
     9481 aaagagagct agattgtaag atagattttg tatacttatt taaaggaaac tttaaatttt
     9541 atatagatga agatattatt tcaaattaca acgataatta tagtattact aaattagaga
     9601 ctatttcaag tgataaagta aatttagtag taaatcatat ttatgttgat ttagaagatt
     9661 atattggaaa taagaaatat gtaaaatata caggaagatt tggaattaat agaagagaac
     9721 aatttagatt aaaagaaatc gttaagagag aaagtaaaaa aataactatg gccagtaaag
     9781 aatctttaaa aaatgaaaaa gaaaagattc tatttttagg tactgaagaa tttatgtata
     9841 tacctatgct gttt
//
LOCUS       DAELLV010000137         9850 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.137, whole genome shotgun sequence.
ACCESSION   DAELLV010000137 DAELLV010000000
VERSION     DAELLV010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9850
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.137"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            465..1373
                     /gene="trxB"
                     /locus_tag="KPD58_002507"
     CDS             465..1373
                     /gene="trxB"
                     /locus_tag="KPD58_002507"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBG0266268.1"
                     /translation="MVDIIVIGAGPAGLTSAIYAMRAGLSVTVFEKNIYGGQVASTSE
                     VENYPAVQKISGVEFSNNIYNQAVAQGVDIQFDEVEEINLEGKVKVVKTSSGEHKAKA
                     VILANGVERRKLGCAGEQEFTGRGVSYCATCDGAFFKDKEVAIVGGGNTALEDALFLA
                     NNCTKVYLIHRRDSFRGEEVLEKSVKARENIEILYSHGVEKIEGEKTVSKIEVKNLKT
                     EEKRTIDVSGIFIAIGLKPNNKMFENVLDLDEGGYIISDESCTTSVEGVYVAGDSRTK
                     FLRQIITAASDGAIAAVQAANYINVE"
     gene            complement(1463..3286)
                     /gene="typA"
                     /locus_tag="KPD58_002508"
     CDS             complement(1463..3286)
                     /gene="typA"
                     /locus_tag="KPD58_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HBG0266269.1"
                     /translation="MSQKHKIINIAVIAHVDAGKSTLVDAFLSQSGVFRKNEVVKDCV
                     MDSNDLEKERGITIYSKNCAINYEDYKINIVDTPGHSDFSSEVERVMKTVDTVILLVD
                     ASEGPMPQTRFVLQKSLEFGLKPILFINKIDKKDQRAEEVVNEVFDLFVDLNATDEQC
                     EFPIIYGIAKQGIAKLEMDDDSEDLSPLFKTIVNHVEAYPNYDNEPLQFQISALAYDD
                     YVGRLGIGRIYKGTLKNNTQVAICREDSVVSKGKVSKLSVYEGLKQVEVDEATSGEIV
                     VIAGIPDISIGETICDLDSPLPMEMIKIEEPTLSMNFLVNDSPFVGKSGKFVTTRHLK
                     DRLEKELEVNVGLKVEPLDTTDGYKVSGRGELHLSILLENMRREGYEVGVSKPEVLMH
                     KEDGKLMEPIERVVVNCPEVYSGTIINELNMRKGMMESMSIEGDYVKIEFLAPTRGLL
                     GYRSEFINATRGEGTLVRSFEKFEEFKGEIPSRGNGVLIAQGPGVTMGYSLNALSDRA
                     VMFVDPGVEVYEGMIIGMNSRKDDMVVNPCKNKKMSNVRASGSDDAIKLSPPRIFTLE
                     EALEFIEDDELVEITPDSIRLRKRFLNEHDRLRYNKSRQGK"
     gene            3498..3740
                     /locus_tag="KPD58_002509"
     CDS             3498..3740
                     /locus_tag="KPD58_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266270.1"
                     /translation="MLEVTKIIIKYFASESDTFVKEEVCNFEELLKWSHKFIEGKKIE
                     DVLYIKSKENYKVNVIKSLEIKELEIVRKQQIPPYM"
     gene            3888..4079
                     /locus_tag="KPD58_002510"
     CDS             3888..4079
                     /locus_tag="KPD58_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HBG0266271.1"
                     /translation="MIRVCPNCSNVDVNKLKTLVGEENVKTGCIGQCRAFKKEAVGII
                     DNELEIKETEAEFFEACKE"
     gene            4363..6864
                     /locus_tag="KPD58_002511"
     CDS             4363..6864
                     /locus_tag="KPD58_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HBG0266272.1"
                     /translation="MSSNKVVESYKITGMTCAACAKAVERVTKKMDGVYDQSVNIATE
                     KLKIEYDNSKVNFDDIKQVVEKAGYGIIKEESNKKIDMKIDGMTCAACAKAVERVVKK
                     LDGVESISVNIATDKANIDYDPSKVKLSQIKAAIEKAGYKPIEEVKNKVDVDEDKLRK
                     EREMKSLFVKFIVAIVFAVPLFYIAMGPMIIKPIGPWPLPEIINPMTNTFNYALIQLI
                     LVIPVMIAGYKFYINGFKSLFSLSPNMDSLVAIGTLAAFLYSLYTTLQIANGQIQGMH
                     HHQLYYESAGIIIALILLGKYLESKSKGKTSEAIKKLMGLQPKTAIVLVDGKEVETPI
                     EEVEIGDILLVKPGTKIPVDGVVIEGYTSVDESMLTGESIPVEKNVGSKVTGASINKN
                     GVIKFKAEKIGGDTALAQIIKLVEDAQGTKAPIAKLADTVSGYFVPIVIAIAVVASLL
                     WFLIGGKDIVFVLTIFISVLVIACPCALGLATPTAIMVGTGKGAENGILIKGGEALES
                     AHKVNTVIFDKTGTITEGKPKVTDIVLNNNVKEEYLIKIASSAEKGSEHPLGEAIVKY
                     GEEKNIKFEKVDNFKAIPGAGIQVTINDESILLGNRKLMNDNNIKLGDLEEKSNILAS
                     QGKTPMYIAVDGNLSGIIAVADVVKESSKKAIEILHDMGIKVAMVTGDNAKTANAIAN
                     QVGIDMVLAEVLPEDKSKEVEKLQNQGKFVAMVGDGINDAPALAKADIGIAIGSGTDV
                     AIESADIVLMKSDLIDVPTAIKLSHETIKNIKQNLFWAFGYNTIGIPVAAGILYVFGG
                     PLLNPMIAAAAMSLSSVSVVSNALRLKNFKAYKRD"
     gene            7024..7692
                     /locus_tag="KPD58_002512"
     CDS             7024..7692
                     /locus_tag="KPD58_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0266273.1"
                     /translation="MNSSILVIEDDSNIQELISEFLSAEGYQVDTANDGLEGIQKFKQ
                     GSYDLVILDIMMPNLDGYGVCKMIRKSSSVPIIFLTALNDEGDQLKGFDLECDDYITK
                     PFSFNLLIKRVEAILRRSNKTINDKFIVFEKLKLDLNTYIAEIDGEPIELTLKEFNIL
                     KALIEKYPQVITREGLLDSIWGYDYYGDTRIVDAHIKNIRKKISLPYIKTVKGIGYTL
                     EKDI"
     gene            7676..8953
                     /locus_tag="KPD58_002513"
     CDS             7676..8953
                     /locus_tag="KPD58_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBG0266274.1"
                     /translation="MRRIFDKWEKLSIKYKLFSITTSLLIALALIIYLILYFLLPSYY
                     HEYKIESLQESLKSLVDSSIHFDTYTLEERLYYMAKDQNLAILLKDNQGKIVYGKNEV
                     VILRYSKYMINSLEDEYRTSIPIYTKDAKDGPYTLELVMPLQPIDEANEVIRKLMPYI
                     ISIAILIAIIGAYIYSIVITKPLINIIESEREQEYRRKDFVATISHELKTPITIISGQ
                     IEGMIYSVGKYKDRDTYLKKSYECTQELKDLVNEMIEVSKSEILEKDLKLVSINISEL
                     LNRLVKRQVFLIEEKHMKTILKIEENLEIKADQERITKAINNIINNAIKYSPEESELI
                     IRLYDKNKRISKKTSNQRVVLEIENTGVTIEKRYLEEIFNPFYRIEKSRSRKTGGSGL
                     GLYIVSQIFKSHGFDYSIKNKENSVVFTVEFKN"
     gene            9354..9575
                     /locus_tag="KPD58_002514"
     CDS             9354..9575
                     /locus_tag="KPD58_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266275.1"
                     /translation="MDRLKRDMKNMKEDLKEGVKKTAYVMKNTAEDLKDDAQGAMVNL
                     EMKKDEMMERYEQKKFSKELEKQMKNEMK"
BASE COUNT         3932 a         1109 c         1658 g         3151 t
ORIGIN      
        1 aaactattaa tcataattgg ttcaacaaaa gtatatagtt aataatgttt aatttgaaga
       61 aattgttagt gtgaaattac atatatattc aaattaaatt cataaatatt tcacataata
      121 atagtatact gtaaacaaaa ggaggtatta aatatgggaa tagtacttat aacagcagca
      181 ttagttttgg ttatgtatgg actttcaatt ctatcatata tatcagaaag caatatatta
      241 gaagacactg taaatgataa ccaataaggt tttgaattat ataaataagt gagaactgta
      301 aataaaagtt aatttttatt tgcagttttt ttatatatat gtaaaataaa gttaattaat
      361 aaaaaataat gttttgttta tagtatatat ggataataat agtaaagtat aagaatgatg
      421 agaataatat ataagaaata ataatataaa gagaggtaaa cgttatggta gatatcattg
      481 taattggtgc tggaccagca ggtttaactt cagcaattta tgctatgaga gcagggttaa
      541 gcgttacagt atttgaaaaa aatatttatg gaggtcaagt tgcaagtacc tctgaggtag
      601 aaaattatcc agcagttcaa aagatttctg gggtagaatt ttctaataat atatacaatc
      661 aagcagttgc tcaaggtgtt gatattcagt ttgatgaggt tgaagagata aatttagagg
      721 gaaaagtaaa ggttgtaaaa acttcttcag gtgaacataa agcaaaggct gttattcttg
      781 caaatggagt tgaaagacgt aaattaggtt gtgcagggga acaagaattt acaggtagag
      841 gagtatctta ctgtgcaaca tgtgatggag ccttttttaa agataaagaa gttgctatag
      901 taggtggagg aaatactgca ctagaagatg ctttattttt agctaataat tgtacaaaag
      961 tgtatttaat tcacagaaga gatagtttta gaggtgaaga agtgttggag aaatctgtga
     1021 aagcaagaga gaatattgag atactttaca gtcatggtgt agaaaaaata gaaggagaaa
     1081 agactgtatc gaaaatagaa gtaaaaaact taaagactga agaaaaaaga actatagatg
     1141 ttagtggtat ttttattgct ataggtctta aacctaataa taaaatgttt gaaaatgttc
     1201 ttgatttgga tgaaggtgga tatataattt cagatgagtc ttgtacaaca tcagtagaag
     1261 gtgtatatgt agcaggagat agtagaacta agtttttacg tcaaataata acagcagcat
     1321 cagatggagc tattgcagcc gttcaagcag ctaattatat aaatgttgaa taatataaat
     1381 ttcaaataaa aatagccata taaactgaaa taatttttca gtactatatg gctattttgt
     1441 taattagagg taaatgtacg ttttatttac cttgtctaga tttgttatat cttaatctat
     1501 catgttcatt taagaatctt tttctaagtc ttatagaatc aggtgtaatt tcaactaatt
     1561 catcatcttc tataaactct agtgcttctt caagagtaaa tattctagga ggagataatt
     1621 ttatagcatc atctgaacca gaagcacgta cgttactcat tttcttgttt ttacaaggat
     1681 tgactaccat atcatctttt ctggaattca taccaattat cataccttcg taaacttcta
     1741 caccagggtc tacaaacatt acagctctat cacttaaggc attaagagaa taccccatag
     1801 taacaccagg tccttgtgct attaaaacac catttcctct tgaaggtatt tctcctttaa
     1861 attcttcaaa tttttcaaat gaacgaacta aagtaccttc tccacgagta gcgtttataa
     1921 attcacttcg atatcctaaa agaccacgag taggtgctaa gaactctatt tttacatagt
     1981 ctccttctat tgacatagac tccatcatac cttttctcat gtttaattca tttataatag
     2041 taccagagta aacttcagga cagtttacaa caactctttc tattggttcc attagttttc
     2101 catcttcttt atgcattaaa acttcaggtt tagaaacacc aacttcataa ccttcacgtc
     2161 tcatattttc aagtagtata gataagtgaa gctctccacg tccagaaact ttgtatccat
     2221 cagtagtatc aagaggttca actttaagac caacatttac ttcaagttcc ttttcaagtc
     2281 tatctttcaa atgtctagtt gtaacgaatt ttccactttt tccaacgaat ggagagtcgt
     2341 ttactaagaa gttcatagaa agagtaggct cttctatttt tatcatttcc ataggtagtg
     2401 gactatctaa atcgcatata gtttcaccta tagatatatc aggaatacca gctataacaa
     2461 ctatttctcc actagtagct tcatcaactt caacttgttt taaaccttca tacacagaaa
     2521 gttttgatac ttttccttta gatacaacag aatcttctct acatatagca acttgtgtat
     2581 tgtttttaag agtacctttg taaattctac ctataccaag tctacctaca taatcatcat
     2641 atgcaagagc tgatatttgg aactgtagag gttcattatc gtaattagga taagcttcaa
     2701 cgtgatttac aatagtttta aatagaggag aaaggtcttc actatcatca tccatttcaa
     2761 gtttagcaat accttgttta gcaataccat atattatagg aaattcgcat tgctcatctg
     2821 ttgcatttaa gtctacaaat aaatcgaaaa cttcattaac tacttcttcc gctctctggt
     2881 cttttttatc aatcttgttt ataaataata ttggttttaa accgaactct aaagattttt
     2941 gtaaaacgaa tctagtttga ggcattgggc cttcactagc atcaactaat agaataactg
     3001 tgtcaacagt tttcataaca cgttcaactt cagaagaaaa gtcactgtgt cctggagtat
     3061 caactatgtt tattttgtaa tcttcatagt ttattgcaca gttttttgaa tatatagtta
     3121 tgcctctttc tttttcaagg tcgttactgt ccataacgca gtctttaact acttcgttct
     3181 ttctaaaaac accgctttga gaaaggaaag catcaaccaa tgttgatttt ccagcgtcaa
     3241 cgtgggctat aactgcgata tttataattt tatgtttttg tgacattttt gttgctccct
     3301 tcgattaaaa taacttatta attatagcac aatatattat taggtacaag gaggaatata
     3361 ttaaattgta ttaatatagt ttaaattgaa taaaactgtt agttttgcat ctgaaaatta
     3421 ttttgtttaa aaaaattttt tagacaaata ataaccttaa tagataaaaa gattaataaa
     3481 agagtaaagg atgatatatg ttggaagtaa ccaaaataat aattaaatat tttgcaagtg
     3541 aaagtgatac ttttgtaaaa gaagaagttt gtaattttga agaacttctt aagtggtctc
     3601 ataaatttat tgaaggtaaa aagatagaag atgttttgta tataaagtct aaagaaaact
     3661 ataaagtaaa tgtgattaag tctttagaaa ttaaggaatt ggaaatagtg agaaaacaac
     3721 agataccacc gtacatgtag tattaataat atataaaatt aatatgtaat actcgcaata
     3781 ggatataaat ggtatatatg gtaataattt atattaaaaa aacttttata aagtttatat
     3841 ttgggtataa aagtattaat tagacattaa ttaggaggaa cttaaatatg ataagagtat
     3901 gtccaaactg ttcaaatgtg gatgtaaata agctaaaaac tttggttgga gaagaaaatg
     3961 taaagactgg ttgtataggt cagtgtagag catttaaaaa agaagctgtt ggaataatag
     4021 acaatgagct tgaaataaag gaaacagaag cagagttttt tgaagcatgt aaagaataaa
     4081 ttttaaataa aatataataa atagagcatc taaattgtat aagcattaaa caatttagat
     4141 gctctatttt tatatataca tttaattatg agtatacttt tctattaaat acaaataaat
     4201 ataactaata catttattta ttaaaaaaat attgatttaa tatacctata gggggtataa
     4261 taaaaatata gatttctaaa taagtaattt atcaatatta catatcaaaa taaaacgtta
     4321 caaattagct gaaaatatat tgaaagaaag gaagatttta ttatgagttc aaataaagtg
     4381 gtagaaagtt ataaaattac aggtatgaca tgtgcagctt gtgcaaaggc agtagagaga
     4441 gtaactaaga agatggatgg agtatatgac caaagtgtaa atatagctac tgaaaaactt
     4501 aaaatagaat atgataattc taaggttaat tttgatgata taaagcaggt tgtagaaaaa
     4561 gcaggatatg gaattataaa ggaagaaagt aataaaaaaa ttgatatgaa aattgatgga
     4621 atgacatgtg cagcttgtgc aaaagctgta gagagagtgg taaaaaaatt ggatggagta
     4681 gaaagtatta gtgtgaatat tgcaacagat aaagctaata tagactatga cccatcaaaa
     4741 gtaaaattat ctcaaataaa agcagctatt gaaaaagctg gatataaacc aatagaagag
     4801 gtaaaaaata aggttgatgt ggatgaagat aaattgagaa aagaaagaga aatgaagtct
     4861 ttatttgtta aatttatagt tgcaatagta tttgcagtac ctctgtttta tatagcaatg
     4921 ggacctatga taataaagcc tatagggcca tggcctttgc cagaaattat aaatcctatg
     4981 acaaatactt ttaattatgc attaattcag ttaattttag taataccagt aatgatagct
     5041 ggttataaat tttatataaa tgggtttaaa tcactttttt ctttaagccc taatatggat
     5101 tctttagttg caataggaac attagccgca tttttatata gtctatatac gacactacaa
     5161 atagccaatg gacaaattca aggtatgcac catcatcagt tgtattatga aagtgcagga
     5221 ataataatag cacttatctt acttggtaaa tatttagaat caaaatcaaa aggaaaaaca
     5281 tcagaagcaa ttaaaaagct tatggggcta caacctaaaa ctgcaatagt gttagttgat
     5341 ggtaaagaag ttgaaactcc tatagaagaa gtagagatag gagatatact attagtaaaa
     5401 ccaggaacta aaatacctgt ggatggagtt gtaatagaag gatatacatc agttgatgaa
     5461 tcaatgctta caggtgaaag tataccagta gaaaagaatg ttggaagtaa ggtgacagga
     5521 gctagtatta ataaaaatgg tgttattaag tttaaggctg aaaaaattgg tggagatact
     5581 gctcttgccc aaataataaa gttagttgaa gatgcacaag gtacaaaagc acctattgct
     5641 aaacttgccg atactgtatc tggatatttt gtaccaatag taattgcaat agctgtagtt
     5701 gcatcattat tgtggttttt aataggagga aaagatatag tatttgtact tactatattt
     5761 atatcagtac ttgtaattgc ttgtccttgt gcattaggat tagcaacacc aactgctata
     5821 atggttggga ctggaaaggg cgcagaaaat ggaatcttga taaaaggtgg agaggcatta
     5881 gaatctgcac ataaagtaaa tacagtgata tttgataaaa caggtactat aacagaagga
     5941 aagccaaaag taacagatat agtattaaat aataatgtaa aagaagaata tcttatcaag
     6001 attgcttcaa gtgcagaaaa aggttcagaa catcctcttg gagaagcaat agttaagtat
     6061 ggagaagaga agaatataaa atttgaaaaa gttgataatt ttaaagccat accaggtgct
     6121 ggtatacaag ttactattaa tgatgaaagt atattacttg gaaatagaaa gcttatgaat
     6181 gacaataata taaagcttgg ggatttagaa gaaaaatcaa atatattggc aagccaaggt
     6241 aaaacaccta tgtatatagc tgtagatgga aatttatcag gtataatagc agttgcagat
     6301 gtagtcaaag aaagtagtaa aaaagcaata gaaatacttc atgatatggg aataaaagtt
     6361 gcaatggtaa ctggagataa tgcgaaaact gcaaatgcta tagcaaatca agtcggtata
     6421 gatatggttt tggcagaggt tttaccagaa gataagtcaa aagaagttga aaaactacaa
     6481 aatcaaggaa agtttgttgc aatggtaggg gatggaataa atgatgcccc agctcttgca
     6541 aaagcagata tagggatagc aataggaagt ggaacggatg tagccattga atctgcggat
     6601 atagtactta tgaagagtga tttaattgat gttccaacag caattaaact tagtcatgaa
     6661 actataaaaa atataaaaca aaacctattt tgggcatttg gatataatac aattggtata
     6721 cctgtagcag caggaatatt atatgtattt ggaggacctt tattaaaccc aatgattgca
     6781 gcagcagcaa tgagcttaag ttcagtttca gtcgtatcaa atgcacttag acttaaaaac
     6841 ttcaaggcat ataagagaga ttagattaat aaattaaata aaatgtatcc aaaagagcta
     6901 tctttaaatt aaaaggatta atttacaaat taagatagct cttttttcat gtaaaattca
     6961 caacaataag ataaaatata agtaatatac gaaattttaa ataaaataga aaggattgat
     7021 agaatgaatt cgtcaatact tgtaattgaa gatgattcaa atatacaaga gttaatatca
     7081 gaatttttaa gtgcagaagg ttatcaagtt gatacagcaa atgatgggtt ggaaggtata
     7141 caaaagttta agcaaggaag ttatgattta gtaatattag atataatgat gccaaactta
     7201 gatggttatg gagtttgtaa aatgataaga aagtcatcaa gcgttccaat tatattttta
     7261 actgctttaa atgatgaagg agaccaactt aaagggtttg acttagaatg tgatgattat
     7321 ataacaaaac cattttcatt taatcttctt ataaaaagag tagaagcaat tttaaggcga
     7381 agtaacaaga ctataaatga caaatttata gtttttgaaa aattaaaatt agatttaaac
     7441 acatatattg cagagataga tggagaacct atagaactta cactaaaaga gtttaatata
     7501 ttaaaagctt tgatagaaaa gtatccacaa gttataacta gggaaggtct tttggatagt
     7561 atatggggtt atgactatta tggagatact aggattgttg atgcacatat aaaaaatata
     7621 agaaaaaaaa tatcattacc atatattaaa actgtaaaag gaataggata tacacttgag
     7681 aaggatattt gataagtggg aaaagttaag tataaagtat aagttatttt caataacgac
     7741 atcattatta atagcattag ctttaataat atatctaatt ttatattttt tattgccatc
     7801 atattatcat gagtataaaa ttgagtctct tcaagaaagt ttgaaatctt tagtagatag
     7861 ttcaattcat ttcgatacat atactttaga agaaagactt tattatatgg ctaaagacca
     7921 aaatttggct attttattaa aagataacca aggtaaaatt gtatatggta agaatgaagt
     7981 agttatttta agatatagta agtacatgat taatagtctt gaggatgagt atagaacttc
     8041 tatacctata tatactaaag atgcaaaaga tggtccatat actttagaac tcgttatgcc
     8101 attacaacca attgacgaag caaacgaagt tataagaaaa ctaatgccat acatcatatc
     8161 aatagcaata ttgatagcga ttattggagc atatatatat tcaattgtaa taacaaagcc
     8221 tcttataaac ataatagaga gtgagagaga acaggaatat agaaggaagg attttgttgc
     8281 tacaatatca catgaactaa aaactccaat aaccataata agtggtcaaa ttgaaggtat
     8341 gatttatagt gtaggtaagt ataaggatag ggatacttat ctgaaaaaat cgtatgagtg
     8401 tacacaagaa ttaaaagatt tagtaaatga aatgatagag gtttcaaaat cagaaatact
     8461 tgaaaaagat ttgaaattgg tatcaataaa tataagtgaa ttattaaata gacttgtaaa
     8521 gagacaagta tttttaatag aagaaaaaca tatgaaaact attttaaaaa tagaagaaaa
     8581 tttggaaatt aaagcagacc aagagagaat aactaaggca atcaataata taataaataa
     8641 tgccataaaa tattctcctg aagaatcaga gcttataatt agactctatg ataaaaataa
     8701 acgaataagt aaaaagactt ctaatcaaag agtggtttta gaaattgaaa atactggtgt
     8761 aactatcgag aaacgttatt tagaagaaat atttaatcca ttttatagaa tagaaaaatc
     8821 tcgttcgaga aaaactggag gaagtggact tggactttat atagtcagcc aaatttttaa
     8881 aagtcatggg tttgattata gcataaaaaa caaagaaaac tctgtagtat tcacagtaga
     8941 atttaaaaat taattactta ttataaagaa tatataactt tcaataatag ttcattttaa
     9001 aatcacaaaa aattcataat gaaactgtat tatataaata taaataagct taaaaccata
     9061 aaatacacca actataatga tgagatttcc ttacatcctc catataagtc atcattatag
     9121 taacccaaca attaagttac tatgaattta aaaaataaca ccgagcatat aatctatgct
     9181 tagtgttatt tttattttga aataaaaaat atttaattta ttgaaatgta tagatttaga
     9241 tagtttaaac tatatttaag acttaaaaat tataattgat attacaaata aatatatgta
     9301 ctttgggaaa aataaatata ggaagtatta tatttacaga ggaggtagtt ttgatggata
     9361 gattaaaaag agatatgaaa aatatgaaag aagatttaaa agaaggtgtt aaaaagacag
     9421 cttatgttat gaaaaataca gctgaagatt tgaaagatga tgcacaaggt gctatggtaa
     9481 acttagaaat gaaaaaagat gagatgatgg aaagatatga acaaaagaaa ttttctaaag
     9541 aattagagaa gcaaatgaag aatgaaatga aataagcttt tatttatgaa taacaaatta
     9601 ttgtattcat ataaaaagta gccagtaata ttaaatagtt tatttaagga aatagacttt
     9661 ctaaaaataa ctgtattaga tttagaaagt ctatttcaaa ttagttttat aaatagacat
     9721 aaggataaag tagatgttat agaactagat tttaaaaaat agatactaag gataaaataa
     9781 atattataga aatatgtttt taagaatatt tatcaaataa aaatttatat ttataagaaa
     9841 tcctataaaa
//
LOCUS       DAELLV010000138         9782 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.138, whole genome shotgun sequence.
ACCESSION   DAELLV010000138 DAELLV010000000
VERSION     DAELLV010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9782
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.138"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            187..1551
                     /locus_tag="KPD58_002515"
     CDS             187..1551
                     /locus_tag="KPD58_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG0266276.1"
                     /translation="MGEKKINNMNYSHNNYSKKTGEPTRKIKNSHITLKSKEEKICSS
                     CNKENNTKDNYCKFCGNELYEIASLRPLETKLDLKSKIKELSYHANKRGVFLTTFTTI
                     FILFIIALIFKAIITIQFNDISYLVNPAHIILALNLGQISVSMSTMMGSGFINANIGL
                     LILLIIPILVLLISNLIFMRKRCRDSKTVLANSLGVGIFYGLVLAILSIFTNVKTSSH
                     SMLEYGYALEYSYEFFSVLLNGFVLGFICTYITTYKKSYEKENMYLSLFSNSIRTFIL
                     GYVLVLVILLVLTISDSSYLNELDMSSYSNGLNLFTILPQIASYMWAFANGISVTIIN
                     STVSMFTLSSSSLFGDTKLMFYAMGALSMLILLLNGYKLRFKYNTDSIRPIIVFSIYY
                     AFLMGILALFSTFILDSNINFFNTTNYGTTLIMQFKVLQAIVISFVYSFVISLIGYKL
                     NSAD"
     gene            1573..2064
                     /locus_tag="KPD58_002516"
     CDS             1573..2064
                     /locus_tag="KPD58_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG0266277.1"
                     /translation="MEGNTNNKVDDKFSKVKKKFGSGKFKVELYKEKSKLNREIDDIQ
                     EEKAKIFLEMGILTYQKIREDSINDNSFDKLCDELLELDKLIYEKNMEINEMEMAESN
                     VTCECGYVGNVNDKYCAECGKKFELENECDDFIICGYCESEIDTEAEFCPCCGRKIII
                     DLE"
     gene            2337..3008
                     /locus_tag="KPD58_002517"
     CDS             2337..3008
                     /locus_tag="KPD58_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBG0266278.1"
                     /translation="MLDKVKGRLIVSCQALEDEPLHSPFIMGRMAKAAMEGGAVGIRA
                     QGVEDIIEIKKVTGLPVIGIIKRNYEDSDIYITPTKKEVDELLTTGCEMIALDATNRV
                     RPNNEDLKELIKYIKENGVLVMADISNYDEAIKAQEYGVDCVSTTLSGYTPYTKTLEG
                     PDFVLMERLVKDLEIPVIAEGKVNTPQDLKKVFELGVHSSVVGSAITRPQLITEKFVK
                     AIEIN"
     gene            3093..3959
                     /locus_tag="KPD58_002518"
     CDS             3093..3959
                     /locus_tag="KPD58_002518"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HBG0266279.1"
                     /translation="MKGIYSALLVSFDKEGNINEKGLRQIIRHNIDVCKVDGLYVGGS
                     TGENFMLSTDEKKRIFEIAKDEVKEEIKLIAQVGSVNLKEAVELAKFTTDLGYDAISA
                     VTPFYYKFDFEEIKHYYNTIINSVDNRLIIYSIPFLTGVDMSLDQFGELFENEKIIGV
                     KFTAADFYLLERMRKTFPNKLIFAGFDEMMLPATVLGVDGAIGSTFNVNGVRARQIFE
                     LTKNEKISEALEVQHVTNDLITDILGNGLYQTIKLLLEEQGVEAGYCRQPMKEATDEM
                     KSRAKEIYRKYF"
     gene            3992..5494
                     /locus_tag="KPD58_002519"
     CDS             3992..5494
                     /locus_tag="KPD58_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HBG0266280.1"
                     /translation="MVGFTWIDFAILVVYLLAVLFAGLLFSKKEMKGKEFFKGDGTIP
                     WWVTSVSIFATLLSPISFLSLAGNSYGGTWILWFAQLGMFIAIPLTIKFFLPLYSRLE
                     IDTAYEYLEMRYESKGLRVLGALMFIIYQIGRMSIIMYLPSIVLAGLTGIPVNVLIIV
                     MGVIAIIYSYTGGLKAVLWTDFIQGMVLIVGVTGTLFYLIASINGGFGTVMDTLTTGH
                     KFLASNEVMFDKNILSTSAFIIFVGAGLNTFSSYVSSQDIVQRFTTTTDIKQLNKMTY
                     GNGVLSMGLATVFYLIGTCLFIYYTQNPDLAQTVQQDQVFASYIAYELPVGITGILLA
                     AIYAASQSTLSTGLNSVATSWTLDIQTIITKDISMEKQTKIAQYISLGVGILSIAVAI
                     VLANGEIKSAYEWFNSFMGLVLGVLAGIFVLGAFCKKATKMGAYVGFIVSAILVVYLK
                     YRMPEVTSWAYSLITITTSVVVGTIVSAIEAKVKAKVSLPKAETTVYYEK"
     gene            5624..6076
                     /locus_tag="KPD58_002520"
     CDS             5624..6076
                     /locus_tag="KPD58_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBG0266281.1"
                     /translation="MIFGNINHSKTYSTLHEDLLRCFEYAKDNSLIDYEKGTYTIDGD
                     DIFVNIVEYKTCEKEDRFWEAHKKYIDVHLMLDGCERIDINFIENLEQKIYEEKGDFL
                     PLDGKNNGYMELRKGDFLVCYPEDAHMTGIKVLEKENIKKAIFKVLVR"
     gene            6236..7039
                     /locus_tag="KPD58_002521"
     CDS             6236..7039
                     /locus_tag="KPD58_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266282.1"
                     /translation="MENIDVFLLIGQSNARGLGNPKESIIPNENCFEYLSTDEIINMR
                     CELETSEGDGTIAPAFSNEWNKLTGNKVCFIHNAKDGSRIKNWNHDNNWFLNDTIEKF
                     NTGCTTLSKHYNIENKYVIWIQGESDAKYGSDVLYYKESLKKIAYRLKEECMIDKMFV
                     SLTGYWLGEDEYFIRTRRIAAAQESACSECDILCVGSKIAMQFHDRGLTIDDVHYTQE
                     GLNMLGEDLCKNIYKYHITKEKSVLSDTIDLSEARKYICELEKISKRFV"
     gene            7097..7984
                     /locus_tag="KPD58_002522"
     CDS             7097..7984
                     /locus_tag="KPD58_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBG0266283.1"
                     /translation="MKNYVCIDVGGTSIKYGFIREDGFIIDKSSLDTEAKEKGGEGIL
                     AKIKDIVKKYIEENEISGICISTAGMVDPVEGKILFALEELIPNYKGMQLKKEVEKEF
                     NIKCEVENDVNCAGLGEMWLGAGRGATSSICLTIGTGIGGCIIINNKLINGFSNSAGE
                     VGYMNVNGSSFQELASTSSLIKKVAKIKNLNENDLNGKIIFDLAKNNDEDCLKELDNM
                     IKSLAVGIANICYVINPEVVILGGGIMAQEKFLKPKIDKALKEVLIERVYKNTNIEFA
                     KRQNDAGMLGALYNFLNKI"
     gene            8003..8851
                     /locus_tag="KPD58_002523"
     CDS             8003..8851
                     /locus_tag="KPD58_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG0266284.1"
                     /translation="MSILEQLESPTFKVTKSDKLLIAYIKENIDDVFYKPISQIAKES
                     NIGEATITRFVKKMNFNGLQDFKVTLAQEISTINKKNIINKNIQNDEPALDTAKKLLS
                     SNVTTLENTVEIINSKDVHDCARLIINAKKVYFIGIGYSGIIAQDSNYKFMRIGLNCV
                     SFDSSHTMIMMSSIMEEGDLIIAISHSGETEEIIKTVKLARANNAKIISITENKNSEL
                     KDISDVHLSYVSGETVLETGSISSKLAQFFIIDLVYTQVVKELSNEAIERKIKTTNAI
                     KLFKNE"
     gene            8914..9603
                     /locus_tag="KPD58_002524"
     CDS             8914..9603
                     /locus_tag="KPD58_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG0266285.1"
                     /translation="MSKLKDYNIDKVIIFNNLSTKTKNQLKEKVKLKRLQKKEILFYE
                     KDSLDSVYILLEGKVSIFKISENGERKVIFILNSGEVINDITLDASKNSTVGCEAFEN
                     SIVAYYSIEEFLEIMQNDFQLTKDIISYMERRIRRLYRQLKNSISIKVDKKLAAKLYR
                     LSREFGVCCGEWTLLNVNITVTYLADMLGCKRETVSRMIKVLQKEELIKIDNKGFYVK
                     ETELSRYFKNN"
BASE COUNT         3948 a          919 c         1704 g         3211 t
ORIGIN      
        1 aattaagcat aataaaatat aaataataaa gttcgcatta acaaactatt taaatatcaa
       61 gaactgttaa aaaacaacag caataaacaa ataaaataga taaatatatc caattaaggt
      121 ataattaagt tttatatgat atagttatat ttgataattt acaatagtaa aacttggagg
      181 gaaaaaatgg gtgaaaagaa aataaataat atgaattatt cacataataa ttattccaaa
      241 aagacaggtg aaccaactag aaaaataaaa aatagccata tcactcttaa aagtaaagaa
      301 gaaaaaattt gttctagttg caataaagaa aataatacta aggataatta ctgtaaattt
      361 tgtggaaatg aactttatga aatagcatca ttaagaccac ttgaaacaaa actagacttg
      421 aaatctaaaa taaaagagct ttcatatcat gcaaataaaa gaggtgtatt tttaacaaca
      481 tttacaacca tatttatatt gtttattatt gcattgatat ttaaagctat tataactatt
      541 caatttaatg atattagtta tttagtaaat ccagcacata taatcctagc tctcaacttg
      601 ggtcaaatta gtgtatctat gtctaccatg atgggttctg ggtttataaa tgccaatata
      661 ggtttgttga tattacttat aatccctatt ttggtattat taatatcaaa cctaattttt
      721 atgagaaaaa gatgtagaga ttcaaaaact gtattagcaa attctctagg agttggaatt
      781 ttttatggat tagttttagc tattttatct atttttacta atgtaaaaac tagttcacat
      841 agtatgttag aatatggata tgcattagaa tatagttatg aattttttag tgttctttta
      901 aatggattcg tattagggtt tatatgtaca tacataacta cttataaaaa aagttatgaa
      961 aaagaaaata tgtatttatc tcttttttca aattctataa gaacattcat attaggttat
     1021 gttttagtat tagtaatact attggtacta actatatctg atagtagtta cttaaatgaa
     1081 ctagatatga gtagttattc aaatggatta aatttattta ctatactccc acaaatagct
     1141 agttatatgt gggcttttgc aaatggaatt tcagtaacca taataaactc aacagtatca
     1201 atgtttactt taagttcttc atctttattt ggagatacta aattaatgtt ttatgcaatg
     1261 ggagcattgt ctatgttaat attattatta aatggataca agctaagatt taagtataat
     1321 acagatagta taagacctat aatagtattt agtatatatt atgcattttt gatgggaata
     1381 ttggctttat ttagtacatt tatactagat agtaatatta atttctttaa tacaactaat
     1441 tatggaacaa ctttaataat gcaatttaaa gtcttacaag ctattgtgat ttcatttgta
     1501 tatagttttg tgatatcttt aataggatat aagctaaatt cagctgatta aatatgattg
     1561 gagggaattt ctgtggaagg aaatactaat aataaagtag atgataaatt ttctaaggtt
     1621 aagaaaaagt ttggtagtgg aaaatttaaa gttgaattat ataaagaaaa atcaaaatta
     1681 aatagagaga ttgatgatat tcaagaagag aaagcaaaga tattcttgga aatgggaata
     1741 ctgacatatc aaaaaattag agaagattct attaatgata atagttttga taaattatgt
     1801 gatgagttac tagaacttga taagttaatt tatgaaaaaa atatggaaat taatgaaatg
     1861 gaaatggcag aaagtaatgt aacttgtgag tgtggttatg ttggaaatgt aaatgataaa
     1921 tattgtgctg aatgtggtaa aaaatttgaa ttggaaaatg aatgtgatga ttttataatt
     1981 tgtggttatt gtgaaagtga gatagataca gaggctgaat tttgtccatg ctgtggaaga
     2041 aaaattataa tagatttaga ataatattgg gaaacctatg tatagtatac atgggttttt
     2101 ttattgaaat tatttaaatg gacttataac agtagttata taaaatgaaa ataaacaccg
     2161 aaaaaatgaa ataaaatttc ttttaaatgt aaaaaacaag ctaatatata aaataaatca
     2221 gtaaaaatat agatgtgaga aaaaaatatg aaaaaatatt gcaaagaaaa agttttcatg
     2281 ttattattaa tacataaatc aagaaaataa atttcagaaa cgaggtaatt taaaaaatgc
     2341 tagataaggt aaagggaaga ttaatagtat catgccaagc tttagaagat gaacctcttc
     2401 atagtccttt tataatgggg agaatggcta aggctgctat ggaaggtgga gctgttggaa
     2461 ttagagctca aggagttgaa gatattattg aaataaaaaa agttactgga cttcctgtta
     2521 taggtattat aaagagaaac tatgaagatt cagatatata tataacgcca actaaaaaag
     2581 aagttgatga acttttaaca acagggtgtg aaatgatagc attagatgct accaatagag
     2641 ttagaccaaa taatgaagac ttaaaagaac ttataaagta tataaaagaa aacggtgttc
     2701 tagtaatggc agatatttca aattatgatg aggctatcaa agcacaagag tacggtgtag
     2761 attgcgtttc aactacacta tctggatata ctccatatac taaaacatta gaaggtccag
     2821 attttgtatt aatggaaaga ttagttaaag atttagaaat acctgtaata gctgaaggaa
     2881 aggtaaatac tcctcaagat ttaaaaaagg tctttgagtt gggtgttcat tcatcagtag
     2941 taggttcagc aataacaaga ccacaattaa taacagaaaa atttgttaaa gcaattgaaa
     3001 ttaattaaaa attaatttat aaattaaaaa attattatat ttaactatat ttgaaaatta
     3061 aaattggagg taaagatatg agaacaacag atatgaaggg aatttattca gcattattag
     3121 tttcatttga taaggaagga aatataaatg aaaaagggtt aagacaaata attagacaca
     3181 atatagatgt ttgtaaagta gatggtcttt atgtaggagg aagtacaggg gaaaacttca
     3241 tgctttcaac tgatgaaaag aaaagaatat ttgaaatagc taaagatgaa gtgaaagaag
     3301 aaataaaatt aatagctcaa gttggttcag ttaatttaaa agaagctgta gaacttgcta
     3361 aatttacaac tgatttagga tatgatgcaa taagtgctgt tacacctttt tactataaat
     3421 ttgattttga agaaataaaa cactattata atacaataat aaattctgta gataatagat
     3481 taataatata ctctatacca tttttaactg gtgttgatat gagtttagac cagtttggag
     3541 aattatttga aaatgaaaaa ataataggtg ttaagtttac agcagcagat ttctacttat
     3601 tagagagaat gagaaaaaca ttcccaaata aattaatatt tgcaggattt gatgaaatga
     3661 tgttaccagc tacagtttta ggtgtagatg gggcaattgg ttctacattc aatgtaaacg
     3721 gtgtcagagc tagacaaata ttcgaattaa ctaagaatga aaaaatatca gaagcacttg
     3781 aagtacaaca tgtgactaat gatttaataa cagatatatt aggaaatggc ttatatcaaa
     3841 caattaaatt actacttgaa gaacaaggtg ttgaggctgg atactgtaga caacctatga
     3901 aagaagcaac tgatgaaatg aaatcaagag caaaagaaat atacagaaag tatttttaat
     3961 aaaatacttt tagtatggga ggattagtcg aatggtagga tttacttgga tagattttgc
     4021 aatactagtg gtttacttat tggcagtttt atttgcagga ttgttatttt caaaaaagga
     4081 aatgaaggga aaagagttct tcaaaggtga tggaactatt ccgtggtggg ttacatcagt
     4141 atcaatattt gccacattat taagtccaat atcattttta tcattagcag gaaattctta
     4201 tggtggtact tggattttgt ggtttgcaca attaggtatg tttatagcaa tacctttgac
     4261 aataaaattt ttcttacctc tatatagtag gttagaaata gatacagcat atgaatactt
     4321 agaaatgaga tacgaaagta aaggtcttag agtacttgga gctttgatgt ttataatata
     4381 tcaaatagga cgtatgtcta ttataatgta tcttccatca atagttctgg ctggtcttac
     4441 aggaatacct gtaaatgtac tgataatagt tatgggtgta atagcaataa tttactcata
     4501 tacaggtgga ttaaaggctg tcttgtggac cgattttata caaggaatgg tattaatagt
     4561 aggtgttaca gggacattgt tctatttaat agcaagtata aatggtggtt ttggaacagt
     4621 aatggatact cttacaactg gacacaagtt cttagcaagt aatgaagtta tgtttgataa
     4681 aaatatacta tctacaagtg catttataat atttgttgga gctggtctta atactttttc
     4741 atcatatgta tcgagtcaag atatagttca aaggtttact acaacaactg atataaagca
     4801 acttaataaa atgacatatg gaaatggtgt tttatcaatg ggacttgcaa cagtatttta
     4861 tctaattgga acttgtttat ttatatacta tactcaaaac ccagatttag ctcaaacagt
     4921 tcaacaagac caagtatttg catcatatat agcttatgaa ttaccagtgg gtataacagg
     4981 aatactactt gcagcgatat atgcagcttc tcaatcaact ttatcgacag ggcttaattc
     5041 agttgctaca agttggactt tagatataca aactatcata acaaaagata taagtatgga
     5101 aaagcaaact aagatagcac aatatatatc attaggtgta ggtatacttt ctatagctgt
     5161 tgcaatagta ttagcaaatg gagaaataaa gtcagcatat gaatggttta atagttttat
     5221 ggggctagta ctaggtgttt tagcaggaat atttgtactt ggtgcatttt gtaaaaaagc
     5281 aactaaaatg ggagcatatg ttggattcat agtatcagca attttagttg tttatttaaa
     5341 ataccgtatg ccagaagtaa cttcttgggc atattctcta ataacaatca cgacttctgt
     5401 agtggtaggt acaatagtta gtgcaataga agctaaagtt aaagcaaaag tatctttacc
     5461 aaaagctgag actactgttt attatgaaaa ataatattta aatgagaaat tgtcttatag
     5521 agtgattaga taaataattt agttactcta taagattgta taatatactt tgtatacaaa
     5581 agtataaaaa tagactttat aattatatag ggagatagtg cttatgatat ttggaaatat
     5641 aaatcatagt aagacatatt caacattgca tgaagattta cttagatgtt ttgaatatgc
     5701 taaagataat tcattaatag attatgaaaa aggtacttat acaatagatg gtgatgatat
     5761 ttttgtgaat attgtagagt ataaaacttg tgaaaaagaa gatagattct gggaagctca
     5821 taagaagtat atagatgttc atttgatgct tgatggatgt gaacgaatag atataaattt
     5881 tatagaaaat ttagaacaaa aaatatatga agaaaagggc gatttcttgc ctcttgatgg
     5941 taaaaataat ggatacatgg aacttagaaa aggtgatttt ttagtttgtt atccagaaga
     6001 tgctcatatg acaggaataa aagttttaga aaaagaaaat atcaaaaaag ctatttttaa
     6061 ggttttggtt agataatgat aatttatata accactaggg ctgaaaatag tgatttaact
     6121 ttcagcgatt agtgagactt cgtaaatttt agttagatta ctattttcag ccttagtttt
     6181 ttatgagaaa attgaaaata attaaaagct taaaattttg taaaggagtt aacttatgga
     6241 aaatatagat gtattcttat taataggaca aagcaatgct agaggattgg gaaatccaaa
     6301 agagagcatc attccaaatg aaaattgttt tgaatatttg agtactgatg aaattataaa
     6361 tatgagatgt gagctagaga cttcagaagg tgatggtact atagcacctg cattttcaaa
     6421 tgaatggaat aagcttactg gaaataaagt ttgttttatt cacaatgcaa aagatggttc
     6481 aagaataaaa aactggaatc atgataataa ctggtttcta aatgacacta tagaaaaatt
     6541 taatacaggc tgtactacct taagtaaaca ttataatata gaaaataaat acgttatttg
     6601 gattcaaggt gaaagtgatg caaaatatgg tagtgatgta ttgtactata aagaaagtct
     6661 aaaaaaaatt gcgtataggc ttaaagaaga atgtatgatt gataaaatgt ttgtaagttt
     6721 aactggttat tggcttggag aagatgaata ttttattaga acaagacgaa ttgcagcagc
     6781 acaggagtcg gcttgtagtg aatgtgatat attatgtgta ggaagcaaaa ttgctatgca
     6841 gtttcatgac agaggattaa ctattgatga tgttcattac actcaagaag gtcttaatat
     6901 gttaggagag gacttatgta aaaatattta taagtatcat ataaccaaag aaaaaagtgt
     6961 attaagtgat actatagact tatcagaagc aaggaaatat atctgtgaat tggaaaagat
     7021 aagtaaaaga tttgtgtaat ataaaatttt aaatagagtt gttaatttat aaaaataatt
     7081 tgtatggaga tatgttatga aaaattacgt ttgtatagat gttggaggta cttcaataaa
     7141 atatggtttt attagagaag atggatttat aattgataaa tcaagtcttg atactgaagc
     7201 aaaggaaaaa ggtggagaag gaatattagc caaaataaaa gatatagtaa aaaaatatat
     7261 agaggaaaat gaaataagtg gtatatgtat atcaactgca ggtatggttg acccagttga
     7321 aggtaaaata ctatttgcat tagaagaatt aattccaaac tataagggca tgcaattaaa
     7381 aaaagaagta gaaaaagagt ttaatataaa atgtgaagtt gaaaatgatg ttaattgtgc
     7441 tggacttggt gaaatgtggc ttggagcagg tagaggtgca acatcttcta tatgtttaac
     7501 aataggtact ggaattggtg gatgtattat aataaataat aaactgataa atgggtttag
     7561 taatagtgct ggtgaagtag gatatatgaa tgtaaatgga agttcttttc aagaattggc
     7621 atctactagc agtctaatta aaaaagtagc aaaaataaaa aatttgaatg aaaatgactt
     7681 aaatggaaaa ataatttttg atttagcaaa gaataatgat gaagattgtt taaaagaact
     7741 cgataatatg ataaaatcat tagcagttgg aatcgcaaat atatgttatg taataaatcc
     7801 agaagtggta attttaggtg gtgggattat ggcacaagaa aagttcctaa aaccaaaaat
     7861 agacaaagca ttaaaggagg ttcttataga gagagtttac aaaaacacta atatagaatt
     7921 tgcaaagaga caaaatgatg caggaatgct tggagcatta tataattttt taaacaaaat
     7981 ataaagaata gaagggtttt taatgagtat tttagaacaa ttggaatcac caacttttaa
     8041 agtcacaaaa tcagataaat tattaatagc ttatataaaa gaaaatatag atgatgtatt
     8101 ttataaacca atatcacaaa tagctaaaga gagcaatatt ggtgaagcaa ctataactag
     8161 gtttgtaaaa aaaatgaatt ttaatggatt acaggatttt aaagttactt tagcacaaga
     8221 aatatcaact atcaataaaa aaaatattat aaataaaaat atacaaaatg atgagccagc
     8281 tttggataca gcaaaaaaac ttttaagttc aaatgtaacc actttagaga acacagtaga
     8341 gataatcaat agtaaggatg tacatgactg cgcaaggctt ataataaatg caaagaaagt
     8401 ttacttcata ggtattggat attctggtat aatagcacag gattcaaact ataaatttat
     8461 gagaataggt cttaattgtg taagttttga cagtagtcat acaatgataa tgatgtcttc
     8521 cataatggaa gaaggagatt taataattgc aatttcacat tctggtgaaa cagaagagat
     8581 tataaaaact gtaaagcttg caagagccaa taatgctaaa attatatcaa ttactgaaaa
     8641 taaaaattct gaattaaagg atatttcaga tgtacactta tcttatgtat cgggagaaac
     8701 agtactggaa accggttcaa tatcttctaa attagcacaa tttttcatta ttgatttagt
     8761 atatacacag gtagtaaaag aactttctaa tgaagctatt gaaaggaaaa ttaaaactac
     8821 aaatgctata aaattgttta aaaatgaata aaataagaaa tttgtaatgt tttatagagc
     8881 tctcttaaaa atttaggaat ggagagatat tacatgtcta aattaaaaga ttataatatt
     8941 gataaagtaa taatttttaa taatttaagt actaaaacga agaatcaact taaagaaaaa
     9001 gttaaactaa aaagattaca aaaaaaagaa attttatttt atgaaaaaga tagcttagac
     9061 agtgtttata tacttttaga aggaaaggta tctattttta agataagtga aaatggagag
     9121 agaaaagtaa ttttcatatt aaatagtggg gaagtcataa atgatattac acttgacgct
     9181 tctaaaaact ctactgttgg ctgtgaagca tttgaaaatt caattgtagc ttattatagt
     9241 attgaagaat ttttagaaat tatgcaaaat gattttcaac ttacaaagga tattatttct
     9301 tatatggaaa gaagaataag acgtttatat agacagttga aaaattctat atccataaaa
     9361 gtagataaaa aacttgcagc aaaattatat aggttaagta gagaatttgg agtttgctgt
     9421 ggagaatgga ctttactaaa tgtaaatatt acagttacat atttagctga tatgcttggc
     9481 tgtaaaaggg agactgtttc cagaatgata aaggtattgc aaaaagaaga acttattaaa
     9541 attgacaata aaggttttta tgttaaagaa actgaattat caaggtattt taaaaataat
     9601 taattatgat taagtaagag tattattaga attttaatta aataaaaata ttattaagag
     9661 aatctattta tagattcttt tttattgtct aatttattaa tttttatttt accctattga
     9721 attgtataaa aaaacaaata agattctttt ttattcaaaa aattgagtag atagtagttt
     9781 ta
//
LOCUS       DAELLV010000139         9626 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.139, whole genome shotgun sequence.
ACCESSION   DAELLV010000139 DAELLV010000000
VERSION     DAELLV010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9626
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.139"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(26..1222)
                     /locus_tag="KPD58_002525"
     CDS             complement(26..1222)
                     /locus_tag="KPD58_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0266286.1"
                     /translation="MLKTNNLSVGYNNKVVISNINVEVKNGEILCLLGSNGAGKTTLL
                     RSLSKLISPIKGEIYLNGVNINCISRKALSKKMALVLTNRLLGDLMTVQDIVNIGRYP
                     YTGFFGSLSKKDLIMVDEALESVDALHLKKRYFDELSDGEKQKVLVARALVQEPEIII
                     LDEPTTHLDIKHRLELINILKKLSKEKSISVILSLHEIDIALKSCDKVALIKNNKVIA
                     YGQPEDVVDEDIINSLYELDDKNFNSLLGSVEISNKSKNEVFIIGGGGKATPIYRAFT
                     KKGIGLYSGIIHENDIDYEIGRTMGIKMFTENPFEPISDESFDLAIRNLNDSKIIIDT
                     GFSVGETNKRNIDIIKEALKLDKKVYSFRNRDESKKYYDSLDNKIEHIDKVSQIINSA
                     DINNLL"
     gene            complement(1225..2250)
                     /locus_tag="KPD58_002526"
     CDS             complement(1225..2250)
                     /locus_tag="KPD58_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBG0266287.1"
                     /translation="MDFINKMRIRNTTIFIVLIISMILTFLVCVAFGSVKIEFKEVID
                     VVLRGMDNTEHYKIIYNIRIPRVLATMVGGACLAVSGILLQIFFKNPIVEPYILGVSS
                     GSTLSVALMILGGYTLGFESVNSMSIFVAAFIGALIVMSIVILFANKVKNITTLLIIG
                     IMTGYVCSGITNVLQSFANSDKLKEFTMWSMGSFSGFTWEKVEILIIVGILGLLMTVF
                     IIKPLNAFLLGEEYAKSIGINIRFFRVLIVFISSILAAIVSAFAGPIGFIGLAVPQIS
                     KLIFKTSDNRVLIPVVIFLGSIVTALCDLISRVILSPVELPISTITSLLGAPIVVMLL
                     VKRKDRA"
     gene            complement(2322..3452)
                     /locus_tag="KPD58_002527"
     CDS             complement(2322..3452)
                     /locus_tag="KPD58_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0266288.1"
                     /translation="MKFKSNVLKQFFLIAMVIFMVGGLIGCSKSDKENKQASTGKKLE
                     LKYASGFSVEDLEDGIKKVTDGDNRELILIPKKIKIPEKYKNANIVRTPVDNVLIAST
                     TQACSLRTIDELDSIKAVTTEEQYWTIKEIKDLMKDGKIHYVGSNLAPDYEKIVDLNP
                     DLTIVSSGLSEADTKFIDKLKELGLNYVVDNEYKEQNPFGRMEWTSLIAAFYDKEDMA
                     TSELNKTVQKVEEVSKKIKNKAKPKVVWASVYEGSAYIAPKDSYVDNMIKMAGGINAC
                     ASIDSNEGRVSIEQLHEVAKDADIFVYSSSSDYAPNLDSIKSSAPVLADLDVVKNGNL
                     WVFAPDYYQSVDKTDELIVDLVEMFHPGTTGEKIKHYINYDK"
     gene            complement(4046..4441)
                     /gene="tnpA"
                     /locus_tag="KPD58_002528"
     CDS             complement(4046..4441)
                     /gene="tnpA"
                     /locus_tag="KPD58_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBG0266289.1"
                     /translation="MKLDSNNHSVFLLHYHLVMCIKYRRKVIDDSISDRLKDIFVDIA
                     PNYGVVLIEWNHYVDHVHLLFKAKPNTEISKLLNAYKSASSRRIKNEFPSIRKYLWKE
                     YFWSKSYCLVTTGGATLEDLEQYIENQEE"
     gene            complement(4606..5613)
                     /locus_tag="KPD58_002529"
     CDS             complement(4606..5613)
                     /locus_tag="KPD58_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003358391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG0266290.1"
                     /translation="MRLREYIGDKVVFLGTNLIIFLIASGIMLYSKINVVLIFMLFCI
                     WFLPLFTYMLIDYLQYKKYFNSIDNILDALDKKYLLPEVIDDVNFVTGEKINDILKVI
                     SRNMHENIKYYNDNQEEYKEYIETWVHEIKTPIASSKLIIENNSNEITKKIDTQLDKV
                     ENFVEQVLYYSKIDNVNKDYIIKKLNLEEVVNEVIKRNYRDFINKNIKLELGEINKTV
                     YSDSKWVEFILNQIVVNSIKYIKSKDGLIKISAKRVENSIVLTIEDNGVGIEERDINK
                     VFEKGFTGENGRVFGKSTGIGLYLCKKLCNKLGLGLILESKVNVGTVIKVSFPIDYNL
                     F"
     gene            complement(5610..6281)
                     /locus_tag="KPD58_002530"
     CDS             complement(5610..6281)
                     /locus_tag="KPD58_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005210744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0266291.1"
                     /translation="MEKIIIVEDEEVIRDELKSFLERYGYEVMAPTEFDNIVEFILSE
                     KPNLVLLDINLPIYDGYYICRVLRKESNIPIIIVTSRNTDMDELMSMNLGADDFVTKP
                     YKTQILLARIVALLKRSGLNSNVNNTLIFKGIELNLFNATLRYKDKEIELTKNEFKIL
                     SYLVENQGSIVSRDSFMEYLWHTNWFVDDRTLTVNITRLRKKLDEIGIENLIETRRGL
                     GYIIS"
     gene            complement(6358..8352)
                     /locus_tag="KPD58_002531"
     CDS             complement(6358..8352)
                     /locus_tag="KPD58_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003400181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0266292.1"
                     /translation="MYSKIAIKNVRKSFKDYSIYFLTLTLAICIFYSFNSIESQKALM
                     EMNASTKSHVEMLKNMISYMSVFVSVILCSLILYANNFLVKKRNKELGIYMTLGMEKV
                     KISKILVFETIIVGIVSLVSGLILGLLVSQGLSILLLKLFEFNMSEYNFVISISSIIK
                     TIVYFGIMFLLVMIFNTYIISKYKIIDLLTIGRKTEDIKFKSPFVYTITFILCIISLI
                     IAYSMVLKVGLNPKNPKFIMSIVLGVIGTILFFYSLAGFALYIVKKNKNIYFKGLNIF
                     VVKQINSKINTNFISISIICLMLFLTIGILSTGFGFKSALNAGLKGATPFDASATMIV
                     HEKCKVKNIKESFEKVGIKFNKGDKVAYYDEYINGNKLSDVMQGQYSGRVDFEVAYIK
                     SSQYNEIRKLRGEKSITLSKDEVLITSNATEVVPSIEEYMKNNKIIKIDNKAYNIKNK
                     KVIKDNLRTDFTETNALTVVVNDKLCDSMEISSSIVNINFASDNNEKSQENFDKRLKS
                     KYSSTSFYEVGTVSGGSRNAMYNDNNGMTSTILFVGIYLGIVFLISSMAVLALQQLSE
                     ASDSIDRYVSLKKLGASKNSISKTIFTQTLMYFSIPVGLALVHSVVGIQVANNFISRL
                     NKPDIGASSLMTVGILMLVYAVYFYTTYQGYKNIVIKKLN"
     gene            complement(8342..9109)
                     /locus_tag="KPD58_002532"
     CDS             complement(8342..9109)
                     /locus_tag="KPD58_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014521021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0266293.1"
                     /translation="MKNILSVEKIEKYYGNKDNITKAIDNISFKVDEGEFVGIMGPSG
                     SGKTTLLNCISTIDNVTIGKIIINNNDITRLKSKNLDKFRQNELGFIFQDFNLLDTLT
                     AYENIALALTIKGEKPSKIDSKIRDVATYLEIEKVIDKYPYQMSGGQKQRVASARAIV
                     TNPSLILADEPTGALDSKSARLLLERFESLNQDLKATILMVTHDAFTASYAHRILFIK
                     DGKIFTELVRGADSRKEFFNRIIEVTTLIGGDDNDVF"
     gene            complement(9364..9591)
                     /locus_tag="KPD58_002533"
     CDS             complement(9364..9591)
                     /locus_tag="KPD58_002533"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266294.1"
                     /translation="MITFVSCFIFLFTVYNTTNHIIINLIDDKQLNSKSKGVEINEFT
                     YQDWQQFCDIKYYGKKGLISMTLKEYIEKYK"
BASE COUNT         3154 a         1650 c          908 g         3914 t
ORIGIN      
        1 acataagtac attttatttc ataaattata ataaattatt tatatctgca ctattgatta
       61 tttgagaaac tttatctata tgttctatct tattatccaa actgtcataa tacttcttac
      121 tctcatctct atttctaaaa gaataaactt ttttatccaa ttttaaagcc tcttttataa
      181 tatctatatt cctcttattt gtctctccta cactaaatcc tgtatctatt ataatcttgg
      241 aatcatttaa atttctaata gccaaatcga aactctcatc acttataggc tcaaatggat
      301 tttctgtaaa catctttatc cccatagttc taccaatttc ataatcaata tcattttcat
      361 gtattattcc agaatacaga cctatacctt ttttagtaaa agctctataa attggtgttg
      421 ctttaccccc accaccaatt ataaaaactt catttttaga tttattacta atttctactg
      481 aacctaaaag actattaaag tttttatcat ccaattcata aagcgaattt ataatatcct
      541 catctactac atcttcaggc tgaccataag ctatgacttt attatttttt ataagagcaa
      601 ctttatcaca actttttaat gctatgtcta tttcatgcaa tgaaagtatt actgatattg
      661 acttttcttt acttaatttt ttcagtatgt ttatgagttc caatctatgt tttatatcca
      721 agtgagttgt aggctcatct aatataatta tttctggttc ttgaacaaga gcccttgcaa
      781 ccaacacctt ttgtttttct ccatcactta gttcatcaaa atatcttttc tttaaatgta
      841 aggcatctac agactccaat gcttcatcta ccattattaa atctttctta gatagactcc
      901 caaaaaatcc tgtatatgga taccttccta tattgactat atcttgtaca gtcattaaat
      961 cacctaataa tctatttgta agtactaaag ccattttttt tgataatgct tttcttgata
     1021 tacaatttat gtttacacca ttcaaatata tttcaccttt tattggtgat ataagtttag
     1081 ataaggacct taatagggta gtttttccag ctccattact tcccaaaaga catagtattt
     1141 ctccattttt tacttctaca tttatattac ttataacaac tttgttattg tatcctacac
     1201 ttaaattatt agttttcaac atgtctaagc cctatccttc cttttcacca ataacattac
     1261 tacaattgga gctcctaata aagatgttat cgtacttatt ggtaattcta ctggtgataa
     1321 aattactctt gatattaaat cacaaagagc tgtaactata ctgcctaaaa atattactac
     1381 tggtatcaaa actctattat ctgatgtttt aaatatcaat tttgaaattt gtggcactgc
     1441 aagtccaata aaccctatag gtcctgcaaa tgctgatact attgctgcta atatactaga
     1501 tataaatact atcaaaactc taaaaaatct tatatttatg ccaatacttt ttgcatactc
     1561 ttctcctaat aaaaaagcat ttaaaggttt tattatgaat acagtcatca aaagacctaa
     1621 tataccaact attataagta tttctacctt ttcccatgta aatccagaaa aacttcccat
     1681 agaccacata gtaaattctt ttaatttatc actatttgca aatgattgta acacatttgt
     1741 tataccacta catacatatc cggtcataat tcctattatt aaaagagttg ttatattctt
     1801 taccttattt gcaaataaaa tgactataga cattacaatt aaagctccta taaaagctgc
     1861 tacaaatata ctcattgaat ttacactctc aaatcccaat gtgtatcctc ccaaaatcat
     1921 gagagctact gataaagtag aacctgaaga tactcctaat atatacggtt caactattgg
     1981 gtttttaaaa aatatctgta ataaaatacc tgatactgct aggcatgctc cacctaccat
     2041 ggttgcaagt actctaggga ttcttatatt gtatattatt ttataatgtt ctgtattatc
     2101 catacctctt aatacaacat ctattacttc tttaaattca atttttacag agccaaatgc
     2161 tacacacact aaaaaagtaa gtatcatact tataattaat actataaata ttgttgtatt
     2221 ccttattctc attttattga taaaatccat ttgtatactc ctcttagcaa agctatatta
     2281 actaaaatct ataaaaccta tctttttgtg tcctctctgt actatttatc ataatttata
     2341 taatgtttga ttttttcacc tgtagtacct gggtggaaca tctcaactaa atctactatt
     2401 aattcgtccg tcttatctac tgattgataa taatctggag caaacaccca taggtttccg
     2461 ttttttacaa catccaaatc agccaataca ggtgcactag attttataga atctaaattt
     2521 ggtgcataat ctgaacttga tgaatataca aatatatctg catcttttgc aacttcatgc
     2581 aattgttcaa tacttactcg cccttcattt gaatctattg aagcacatgc atttattcca
     2641 ccagccattt ttatcatatt atccacatat gaatctttag gagctatata agcacttcct
     2701 tcatatacag atgcccatac aactttaggt ttagctttgt tttttatttt tttacttact
     2761 tcttctacct tttgtacagt tttatttagt tcactagtag ccatatcttc tttatcataa
     2821 aatgcagcta ttaaacttgt ccactccatt cttccaaatg gattttgctc tttatactca
     2881 ttgtctacaa cataattaag ccctaattct tttaatttgt caataaattt tgtatcagcc
     2941 tctgataaac cactactcac tatagttaag tcaggattta agtctactat tttttcataa
     3001 tctggtgcta aatttgaacc aacgtaatgt atttttccat ctttcattaa atctttaatt
     3061 tctttaatag tccagtactg ctcttctgtt gttacggctt ttatactatc cagttcatct
     3121 attgttctca gagaacaagc ctgtgttgta gatgctatta atacattgtc cacaggcgtt
     3181 ctaactatat tagcattttt atatttttca ggaattttaa tcttctttgg gattaaaatc
     3241 agttctcgat tatctccatc agttactttt ttaattccat cttctaaatc ttcaacacta
     3301 aatccactag catattttaa ttctaatttc ttaccagtac tagcttgttt attctctttg
     3361 tcacttttac tacaccctat aagacctcct accataaaaa ttaccatagc aattaaaaaa
     3421 aactgcttta aaacatttga tttaaatttc atctctttca ctccaatatc ttatttttta
     3481 tgagtggcta aaaaacttta ataagatata ccttttaaag tatatcttat taaagttata
     3541 ttaaaccatc tttaacaagt ataacttttt aataaaatta tagtgaattt tgatttcaca
     3601 tgcaattaac tacttataca cttaactacc tacgacaacg tttgaagtgg catcttcttt
     3661 gtcaaaatag ctattgttat tagtatctcc aagctaatag gatagttccc aacacttacg
     3721 attagcatct gagtcactac gttggcaaac taatagaaat ataacattta tttgatgatg
     3781 ctttagatac agtacaactc tatcaaattg cctatgttgt tttatcctaa tcattgattt
     3841 taatttaagc agttttatat acccatttct atatgcacaa tatcttttga gtaacgtgtt
     3901 gacaaactat tttggttagt agtatagcta tgatagttaa ttttctttga tagataagtc
     3961 cgttacatcc aaaattctta ggaaaatatt ccttttattc actgtttgta taaacctgta
     4021 cttataagct cttaacatat tatcactatt cttcttgatt ttctatgtat tgttccaaat
     4081 cttccaatgt tgcaccacca gttgttacta aacaataact tttactccaa aagtattctt
     4141 tccataagta tttgcggata cttggaaact cattctttat ccttctgctt gatgcagatt
     4201 tataggcatt taataacttg gaaatttctg tattaggctt tgctttgaat aataagtgaa
     4261 catggtcaac ataatgattc cattctatca acaccacccc ataatttggt gcaatatcta
     4321 caaatatatc ttttagtcta tcagatatag aatcatctat tacttttctt ctatatttta
     4381 tacacataac taaatgataa tgcaataaga agactgaatg attattacta tctaatttca
     4441 ttataattac ttcccttcag cttttactac taaattaatg atataatacg tttttatgaa
     4501 aatcaagtaa aaaaaagaac ctatcaatta tgcttacatt cttaactttc ttaaataaat
     4561 acaaaaaact gacaactaaa aattgtcagt ttttataact atacactaaa ataaattgta
     4621 atctataggg aatgatacct ttatgactgt accaacattt acttttgatt ctaatattaa
     4681 acctaaacct aacttattac ataacttttt acatagatat aatcctattc cagtactttt
     4741 tccaaatact cttccattct ctcctgtaaa ccctttttca aataccttat taatatctct
     4801 ctcttctata cctactccat tatcttctat agttaacact attgagtttt ctaccctttt
     4861 tgcacttatt tttattaaac catcttttga ctttatatat tttatagaat ttaccacaat
     4921 ttgatttaat ataaactcaa cccatttcga gtcactatat acagtcttat taatttcacc
     4981 aagttctaat tttatatttt tgtttataaa gtctctatag tttcttttga taacttcatt
     5041 tacaacttct tcaaggttta gcttttttat tatataatct ttatttacat tatcaatttt
     5101 tgaataatat aatacttgtt ccacaaaatt ttcaacttta tctagttgag tatctatctt
     5161 tttagtaatc tcattactat tattttctat aataagttta ctagatgcta taggcgtttt
     5221 tatttcatga acccaagttt caatatattc tttatattct tcttgattat cattataata
     5281 tttaatattt tcgtgcatgt ttctacttat tacttttaaa atatcattta ttttttctcc
     5341 agttacaaaa tttacatcat caataacttc tggcaacaaa tattttttat ctaaagcatc
     5401 taaaatatta tctatagaat taaagtactt cttatattgt aaataatcaa ttaacatata
     5461 agtaaataat ggtaaaaacc atatacaaaa taacataaat attaaaacga catttatttt
     5521 actataaagc atgatacctg atgcaattaa aaatataatt aaattagtac ctagaaaaac
     5581 aactttatca cctatatact ctcttaatct catgatatta tataccctaa accacgtctt
     5641 gtctctatca aattttctat gcctatttca tctagctttt tacgtagcct tgttatattg
     5701 acagtaagag ttctatcgtc tacaaaccaa tttgtgtgcc atagatactc cataaatgaa
     5761 tctctagata ctattgaacc ttgattctcc actaaataag atagtatttt aaattcattt
     5821 ttagttaact ctatttcttt atctttatat ctgagtgttg cattaaataa atttaattca
     5881 atacccttaa atattaaagt attatttaca ttcgaattta atccacttct ttttaacaat
     5941 gcaacaatcc ttgctaaaag tatttgtgtt ttatatggct ttgtcacaaa atcatctgct
     6001 cctaaattca tgctcattag ttcatccata tcagtgtttc tacttgttac aattattatt
     6061 ggtatattcg attctttacg taatactcta catatataat atccatcata tattggcaag
     6121 tttatatcaa gtaatactaa attaggtttt tcacttaata taaactctac tatattatca
     6181 aattcagtag gagccataac ttcatatcca tacctctcta aaaagctttt taattcatct
     6241 ctgattactt cttcatcttc cactataatt attttttcca ttaatttttc accctttatt
     6301 ttcaaaaaat ctatttacag tatgattttt actgtaaaca gattttagtt agttgtatta
     6361 attaagcttt tttataacta tatttttata cccttgataa gttgtataaa aatacactgc
     6421 atatactaac attaaaattc ctactgtcat aagtgatgaa gctcctatat ctggcttatt
     6481 aagccttgat ataaaattat tagccacttg tattcctact actgagtgaa ctaaagctag
     6541 tccaacaggt atgctaaagt acattagagt ttgtgtaaat atagttttac ttatactatt
     6601 tttacttgct cctagttttt ttaaggatac atatctatct atactatcac tagcctcact
     6661 taattgctga agtgcaagga ctgccatact acttattaaa aatactattc ctaaatagat
     6721 tccaacaaat agtattgtag aagtcatacc attattatca ttatacatag catttcttga
     6781 tccacctgat acagttccaa cttcataaaa acttgtagaa ctatactttg attttagcct
     6841 tttatcaaaa ttttcttgtg acttttcatt attatctgaa gcgaagttta tatttactat
     6901 acttgaagat atctccatgc tatcacacaa tttgtcattt acaactacag taagagcatt
     6961 tgtttctgta aaatcagttc ttaaattatc ttttattact tttttatttt ttatattata
     7021 tgctttatta tctattttta taattttatt atttttcatg tattcttcta tcgaaggtac
     7081 tacctctgta gcattagatg ttattaatac ttcatctttt gataaagtta ttgacttttc
     7141 acctcgtaac tttcttattt cattatactg tgaagatttt atatatgcta cttcaaagtc
     7201 cactctacca gaatattgtc cttgcataac atctgaaagt ttattaccat ttatatattc
     7261 atcatagtaa gcaactttat cacctttatt aaacttaatt ccaacctttt caaaagattc
     7321 ttttatattc ttcactttac atttttcatg tacaatcatg gttgcacttg catcaaatgg
     7381 agttgcacct tttagtcctg catttaaagc tgatttaaaa ccaaaccctg ttgatagtat
     7441 tcctattgtt aaaaataaca ttaaacaaat aatagatatt gatataaagt ttgtattaat
     7501 cttactattt atttgcttta ctacaaatat gtttaatccc ttaaagtaaa tgttcttatt
     7561 cttcttaaca atatataatg caaagcctgc taaactatag aagaataata tagttccaat
     7621 tacacctaat actattgaca taataaactt aggattttta ggatttaatc caacttttaa
     7681 aaccatgcta tatgcaataa ttaaggaaat aatgcataat ataaatgtaa ttgtatatac
     7741 aaatggactt ttaaatttta tatcctctgt ttttcttcct attgttaata aatctattat
     7801 tttatattta gaaattatat aagtattgaa tatcatcaca agtaaaaaca ttattccaaa
     7861 atagacaata gttttaataa ttgatgatat cgatattaca aaattgtatt cactcatatt
     7921 aaattcaaat aactttaata ataatattga taacccttga gatactaata acccaagtat
     7981 tagtccactt actaaagaaa ctattccaac tattatagtt tcaaaaacta atattttcga
     8041 tattttaact ttttccatcc ctaaagtcat ataaattcca agttccttat tccttttttt
     8101 gactaagaag ttattagcat aaagaattaa actgcataaa ataactgata caaatacaga
     8161 catgtatgaa atcatattct taagcatttc tacatgagat tttgttgaag cattcatttc
     8221 cattaaagct ttttgagatt ctatagagtt aaaactatag aaaatacaaa ttgctaatgt
     8281 taaagtcaaa aaatatatgc tataatcctt aaagcttttt ctaacatttt taattgctat
     8341 tttagaatac atcgttatca tctcctccta ttagagttgt tacttcaatt attctattaa
     8401 aaaactcttt tcttgaatca gctcctctta ctaattctgt aaatattttt ccgtctttta
     8461 taaataggat tctatgggca taactagctg taaaagcatc atgagttacc attaaaattg
     8521 ttgcttttaa gtcttgattt agactttcaa acctctcaag taataatctt gctgattttg
     8581 agtcaagtgc tcctgtaggt tcatctgcca aaatcaatga tggattagta actattgctc
     8641 tagctgatgc tactctttgc ttttgtcctc ctgacatttg ataaggatat ttatctatca
     8701 ctttttctat ttctaagtat gtagctacat ctcttatttt tgaatcaatt ttagatggtt
     8761 tttctccttt aattgttaat gccaatgcta tattttcata agctgttaaa gtatctaaaa
     8821 gattaaaatc ttggaatata aatcctaatt cattttgtct gaacttatct aagtttttag
     8881 attttaatct tgtaatatca ttattattta ttattatttt acctattgtt acattgtcta
     8941 ttgttgatat acagttaaga agtgttgtct tcccactacc acttggaccc attatcccaa
     9001 caaattctcc ttcatcaact ttaaagctta tattatctat tgcttttgta atattatctt
     9061 tgtttccata atatttttct attttttcaa cacttaatat attcttcatg ttctatttct
     9121 cctttaaatt aattttctta tatctctatt gtaatttttt atttaatttt tttaaattga
     9181 tttacatttc attaatgttt catttttgta gtaattatat attattccct aataattact
     9241 aatataaaat actcaagacc agcattcttt cttttatact ttgctagagt aacaaaattt
     9301 actcaattct agaaaacaca aaaatataga gggcaatgcc ttccatctaa aatttatttt
     9361 attctacttg tatttttcta tatattcttt taaagtcatt gatattaaac ctttctttcc
     9421 ataatacttt atatcacaaa attgttgcca atcttgatat gtaaattcat ttatttctac
     9481 acctttagac ttggagttta attgtttatc atctattaga ttaattatga tatgatttgt
     9541 agtgttatat acagtaaata agaaaataaa gcatgataca aatgtaatca tatatacaaa
     9601 tggattttta aattttatat cctctg
//
LOCUS       DAELLV010000140         9548 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.140, whole genome shotgun sequence.
ACCESSION   DAELLV010000140 DAELLV010000000
VERSION     DAELLV010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9548
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.140"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            241..2001
                     /gene="prdR"
                     /locus_tag="KPD58_002534"
     CDS             241..2001
                     /gene="prdR"
                     /locus_tag="KPD58_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator
                     PrdR"
                     /protein_id="HBG0266295.1"
                     /translation="MFSIPEVKKVEEVMDTKFTTIDEDTRIEDAIKEMIKSNTKTLMV
                     IDSSDQLKGIISMTDIHNLYEMHKKYEGQPVKLIMKKDVIYVNEGLTLDECRDIMILK
                     NIGILPVLRDNKIIGVLKQEHIRDYLYMHLEDYGLTLKYIIGQIKEGICAINNEGVVI
                     LWNKFMEERYDIKSEDIVGRPMNEFLENTISEKVLNSKVGMSDLYFTDKKENMYALVH
                     ANPIFYKEEFIGVVCTEVDVTEAKILALELEKVNDTLKYLKNEVKNLSKGSFDKILGK
                     SYKLEKSKAIAKQVARTNSSIFIWGESGTGKEVFARAIHDYSERKGQFIPVNCSAIPN
                     ELFESEFFGYESGAFTGASKKGRIGIFELAKDGTVFLDEIADLPLSMQAKLLRVLQEK
                     EIRRVGGDTTIKINPRIISATNKDLSKMVKAEKFREDLYYRLNVVEIKIPPLRERKED
                     IGLLVHSFLEEICKQNNKPVLTISKDVIDIFQNYRWKGNIRELKNTIENIVVLSQNSK
                     IDVDDVPSYMMDSTNNSTEEEEYPLDLTKATQKIEIKNITKALKMSNGNKAKAAKILN
                     IPRTTLYYKIDQYKIDVSKI"
     gene            2430..4310
                     /gene="prdA"
                     /locus_tag="KPD58_002535"
     CDS             2430..4310
                     /gene="prdA"
                     /locus_tag="KPD58_002535"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-proline reductase (dithiol) proprotein PrdA"
                     /protein_id="HBG0266296.1"
                     /translation="MSITLETAQAHANDPAVCCCRFEAGTIIAPENLEDPAIFADLED
                     SGLLTIPENGLTIGQVLGAKLKETLDALSPMTTDNVEGYKAGEAKEEVVEETVEEAAP
                     VSEAAVVPVSTGVAGETVKIHIGEGKNISLEIPLSVAGQAGVAAPVANVAAPVASAAA
                     EVAPKVEEKKLLRSLTKKHFKIDKVEFADETKIEGTTLYIRNAEEICKEANETQELVV
                     DMKLEIITPDKYETYSEAVLDIQPIATKEEGELGSGITRVIDGAVMVLTGTDEDGVQI
                     GEFGSSEGELNTTIMWGRPGAADKGEIFIKGQVTIKAGTNMERPGPLAAHRAFDYVTQ
                     EIREALKKVDNSLVVDEEVIEQYRREGKKKVVVIKEIMGQGAMHDNLILPVEPVGTLG
                     AQPNVDLGNMPVVLSPLEVLDGGIHALTCIGPASKEMSRHYWREPLVIRAMEDEEIDL
                     VGVVFVGSPQVNAEKFYVSKRLGMLVEAMEVDGAVVTTEGFGNNHIDFASHIEQIGMR
                     GIPVVGVSFSAVQGALVVGNKYMTHMVDNNKSKQGIENEILSNNTLAPEDAVRIMAML
                     KNAIEGVEVKAPERKWNPNVKLNNIEAIEKVTGEKIVLEENEQSLPMSKKRREIYEKD
                     EN"
     gene            4391..4675
                     /locus_tag="KPD58_002536"
     CDS             4391..4675
                     /locus_tag="KPD58_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266297.1"
                     /translation="MDRLKYISSETFYEGIIVDIKGGGVTIDLKGRLGQFKIPNRMLI
                     TDYELKIGQEVGFMLSYPEVLSPEPNQEYVENIRREKEKQAEMQSKQEEK"
     gene            4713..5438
                     /gene="prdB"
                     /locus_tag="KPD58_002537"
     CDS             4713..5438
                     /gene="prdB"
                     /locus_tag="KPD58_002537"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:5163..5165,aa:Sec)
                     /transl_table=11
                     /product="D-proline reductase (dithiol) protein PrdB"
                     /protein_id="HBG0266298.1"
                     /translation="MSLTTVQGLQSEIFVPITPPPVWTPVTKELKDMTIALATAAGVH
                     LKSDKRFNLAGDTTFRAIPNTATVDEMMVSHGGYDNGDVNKDINCMFPIDRLHELAAE
                     GFIKGVAPMHYAFMGGGGNQHVFTEETGPAIAAKLKEEGVDGVVMTAGUGTCHRTAVI
                     VQRAIEEAGIPTIIIAALPPVVRQNGTPRAVAPLVPMGANAGEPHNIEMQTHILRDTL
                     EQLVAIPSAGKIVPLPYEYKAHV"
     gene            5518..6276
                     /gene="prdD"
                     /locus_tag="KPD58_002538"
     CDS             5518..6276
                     /gene="prdD"
                     /locus_tag="KPD58_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase cluster protein PrdD"
                     /protein_id="HBG0266299.1"
                     /translation="MEEKIMRRLVIKPFHMNEVNFGSKTSIKKDVLTIDLSSIDEIKE
                     REELITDIKVDIIKPGDYDREINTIMDIIPISTKVLGRLGEGITHTLTGVYVMLTGAD
                     EDGNQMHEFGSSEGILKDQMVFGRYGTPSVEDYIIHVDVTLKGGLPFERTLPLAAFRA
                     CDDFIQEIRASLKMEDGRNATQVREYFDKIRPNAKKVVIVKQIAGQGAMYDNQLFSKE
                     PSGFEGGTSIIDMGNVPMIISPNEYRDGALRAMT"
     gene            6291..6758
                     /locus_tag="KPD58_002539"
     CDS             6291..6758
                     /locus_tag="KPD58_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HBG0266300.1"
                     /translation="MGIGPSTKETSLHHFRDPLLDIVESDKDVDLLGVIVVGTPDGNE
                     NKTFVGQRTAAWLEAMRVDGAIVSSDGWGNSHVDYANTFEEIGKRDIPVVGVTFNGTQ
                     AKFVVSNQYMDTIVDMNKSKEGIETEVVGENNTNEIDAKKALAFLKLKMRKHG"
     gene            6776..7258
                     /locus_tag="KPD58_002540"
     CDS             6776..7258
                     /locus_tag="KPD58_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HBG0266301.1"
                     /translation="MSFVGISTSMKNTSLHIFRDPLLDLIDNDSDIDLLGVVVVGTSE
                     HNEWKTFLATRLGRWIESLRPDGVIITLDMAGNQHIDFTNAIAEFVKSDIPTVGLTIM
                     GADGLVITNPYLDKATIIDYKKTTGYIETEVVGDNHMDEVDVKKALAFLKLKMRKDAK
                     "
     gene            7278..8285
                     /locus_tag="KPD58_002541"
     CDS             7278..8285
                     /locus_tag="KPD58_002541"
                     /EC_number="5.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase"
                     /protein_id="HBG0266302.1"
                     /translation="MKFSRSIQAIDSHTAGEATRIVVGGIPNIKGNSMPEKKEYLEEN
                     LDYLRTAIMLEPRGHNDMFGSVMTQPCCPDADFGIIFMDGGGYLNMCGHGTIGAMTAA
                     IETGVVPAVEPVTHVVMEAPAGIIRGDVTVVDGKAKEVSFLNVPAFLYKEGVEVDLPG
                     VGTVKFDISFGGSFFAIIHASQLGLKIEPQNAGKLTELAMKLRDIINEKIEIQHPTLA
                     HIKTVDLVEIYDEPTHPEATYKNVVIFGQGQVDRSPCGTGTSAKLATLHAKGELKVGE
                     KFVYESILGTLFKGEIVEETKVADFNAVVPKITGSAYITGFNHFVIDEEDPLKHGFIL
                     K"
     gene            8405..9286
                     /locus_tag="KPD58_002542"
     CDS             8405..9286
                     /locus_tag="KPD58_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HBG0266303.1"
                     /translation="MVAILKALYVVFAAIFAVVFGKDVAKANKEGKLEEQSTPKVAVT
                     GFITDFFDTLGIGSFAPTIAMSKALKLNIPDKKMPGTLNVAHTIPVVTEAFIFTSIIP
                     VDGVTLVSMVVAAAVGSYIGAGIIAKMDERKIQLVMGVTLALTAILMLLGHPWINVLP
                     GGGNELGLTGAKLVIGVIGNFILGALMTAGIGLYAPGMAMVYFLGMSAKVAFPIVMGS
                     CALLMPVASMKFIKEDAYTKKASVIIAICGLIGVFVAAYIVKTLPMDILKALVIVVIA
                     YTSATMIMAANKNKKTA"
BASE COUNT         3756 a         1158 c         1892 g         2742 t
ORIGIN      
        1 tgaatagaaa aatgttttga aggtgaaaat agtattgaaa aaataatata ctaagggtat
       61 aattcatttg atttcatttg tataaattta attataaatt ttaaagtgat tatataaatt
      121 taaatgctaa ttacttaata aattttaata ataattattt aataaataat tattataata
      181 tgctagaata aatatatata aataagtttt gatgaggtac tactataaag gggggacttt
      241 atgttttcga taccagaagt aaaaaaagta gaagaggtaa tggatacaaa atttacaact
      301 atagacgaag acacaagaat tgaagatgca ataaaagaaa tgataaaaag caacacaaaa
      361 actctaatgg taatagattc atctgaccaa ttaaaaggca ttatatccat gacggatata
      421 cataatcttt atgagatgca taaaaagtat gaaggtcaac cagtaaaact tataatgaag
      481 aaggatgtaa tatatgtcaa cgaaggttta accttagatg aatgtagaga tataatgata
      541 ttaaaaaata tagggattct tcctgttctt agagataata aaattattgg agttctaaaa
      601 caagagcaca ttagggacta tctatatatg catttagaag attatggatt aactttgaag
      661 tatattatag gtcaaattaa agaaggaatt tgtgctataa ataacgaagg tgttgttatc
      721 ttgtggaata agtttatgga ggaaaggtat gatataaagt ctgaagatat agttggtcga
      781 ccgatgaatg aatttttgga aaatactatt tcagaaaaag tactaaatag taaagttgga
      841 atgagtgact tgtattttac agataaaaaa gaaaatatgt atgcattagt tcatgcaaat
      901 cctatatttt ataaagaaga atttatcggt gttgtatgta cagaagttga tgttacagaa
      961 gctaagatat tagctcttga acttgaaaaa gtaaatgata cattaaaata tctaaagaac
     1021 gaagtcaaaa atttatctaa aggtagtttt gataagattt taggtaaaag ttataaattg
     1081 gaaaaatcga aagcgattgc aaaacaagtg gctagaacaa atagtagcat atttatttgg
     1141 ggcgaaagtg gtacaggtaa agaagtattt gcaagagcta ttcatgatta tagtgagaga
     1201 aaaggtcaat ttatacctgt taactgtagt gccataccaa atgagttatt tgaaagtgag
     1261 ttttttgggt atgaatcagg tgcgtttaca ggagcaagta aaaagggtcg aataggtata
     1321 tttgaacttg caaaagatgg aactgtattt ttagatgaaa ttgccgattt acctcttagt
     1381 atgcaagcaa agttgcttag agtattacag gaaaaagaaa taagaagagt tggtggagat
     1441 accacaataa aaataaatcc tagaataata tcagctacca ataaagattt gagtaaaatg
     1501 gtaaaggcag aaaaatttag agaagattta tactatagat taaatgttgt tgaaataaag
     1561 attcctccac ttagagagag aaaagaagac ataggattat tggttcatag ctttttagag
     1621 gagatatgta aacaaaataa taagcctgtt cttacaatta gcaaagatgt aatagatata
     1681 tttcagaatt atagatggaa aggtaatatt agagaattaa aaaatacgat tgaaaatatt
     1741 gttgtattgt cacaaaattc taaaatagat gtggatgatg taccaagtta tatgatggat
     1801 tctacaaata atagtactga agaagaagag tatcctttag atttaacgaa ggcaactcaa
     1861 aaaattgaaa taaagaatat aaccaaggca cttaaaatgt ctaatggaaa taaagctaaa
     1921 gcagctaaga tattaaatat acccagaaca actttgtatt ataagataga ccaatacaaa
     1981 atagatgtgt ctaaaatatg acacatgact aaattttgac aaaatgataa actttaattt
     2041 aaaacctagt aaagccaacg cttatataag tgttgtgtat atagatgtgt taaaaaaaat
     2101 gaaaaagtgt tgattaattg acactttttt tgtttgagaa aatgtttaca aatacaaaaa
     2161 ataacatatt ttaaataaat atttatataa tatttggcta aaaatagcga tagatgaaga
     2221 atctattata gaaaaaatct ttttgttaat aagttggcat aggaattgct tataatataa
     2281 agtgacttta agtgagaaaa aaataatatt aatcataaca aattatgtag gttttaattt
     2341 acttaatttc tcagttaagg ggttacctgt tgaggaattg agatttaaaa taataaataa
     2401 gaaaataatg taaggaggta ttttatacta tgtcaataac tttagaaaca gctcaagccc
     2461 atgcaaatga cccagcagtt tgttgttgta gatttgaagc gggaacaatt atagcgccag
     2521 aaaacttaga agatccagca atatttgcag acttagagga ttctggatta ttaacaatac
     2581 cagaaaatgg attaactata ggtcaagtac taggagctaa gttaaaagaa actttagatg
     2641 cactttctcc aatgactaca gataacgtag aaggatacaa agcaggagag gctaaagaag
     2701 aagtagtaga agaaacagta gaagaagcag ctccagtatc agaagcagca gtagttccag
     2761 taagcacagg agttgcaggt gaaacagtta aaatacacat aggtgaaggt aagaacataa
     2821 gcttagagat acctttatca gtagctggtc aagcaggagt tgctgctcca gtagcaaacg
     2881 ttgctgctcc agtggcaagt gcagcagcag aagtagctcc aaaagttgaa gaaaagaaac
     2941 ttttaagaag cttaactaaa aaacacttta aaatagataa agttgaattt gctgatgaaa
     3001 ctaaaataga aggaactact ttatacatca gaaacgcaga agaaatatgt aaagaagcta
     3061 atgaaactca agagttagtt gtagatatga agttagaaat aataactcct gataaatatg
     3121 aaacttacag tgaagctgta ttagatatac aaccaatcgc tactaaagaa gaaggcgaat
     3181 taggttcagg tataactaga gttatagatg gagctgtaat ggtattaact ggtacagatg
     3241 aagatggagt tcaaataggt gaatttggtt cttcagaagg tgagttaaat actactataa
     3301 tgtggggtag accaggtgct gctgacaaag gtgaaatatt catcaaaggt caagtaacaa
     3361 taaaagcagg aactaacatg gaaagaccag gacctttagc tgctcaccgt gcatttgact
     3421 atgtaactca agaaataaga gaagcattaa agaaagttga caactcttta gtagttgatg
     3481 aagaagtaat agagcaatac agaagagaag gtaaaaagaa agttgttgtt ataaaagaaa
     3541 taatgggaca aggtgcaatg catgataacc taatattacc agttgagcca gttggtacat
     3601 taggagctca accaaacgtt gacttaggaa acatgccagt tgtattatct ccacttgaag
     3661 tattagatgg tggtatccat gcattaactt gtataggacc tgcatcaaaa gaaatgtcaa
     3721 gacattactg gagagagcca ttagtaataa gagctatgga agacgaagaa atagatttag
     3781 taggtgttgt atttgttggt tctccacaag taaatgctga gaaattctat gtatctaaga
     3841 gattaggtat gttagttgaa gctatggaag ttgatggagc tgtagtaact actgaaggtt
     3901 tcggaaacaa ccatatagat ttcgcatctc acatagagca aataggtatg agaggtatac
     3961 cagtagttgg tgtaagtttc tcagctgttc aaggtgctct agttgttggt aataaataca
     4021 tgactcacat ggtagacaac aataagtcta agcaaggtat agagaatgaa atattatcta
     4081 acaacacttt agctccagaa gatgctgtta gaataatggc tatgcttaaa aatgctatag
     4141 aaggtgtaga agttaaagct cctgaaagaa aatggaatcc aaatgttaaa ttaaataaca
     4201 tagaagctat agaaaaagtt acaggagaaa aaatagtatt agaagagaat gagcaatctc
     4261 taccaatgag taagaagaga agagaaatat acgaaaaaga cgaaaactaa aatttagata
     4321 ctaactaaga tttagacaat aacataaatt tagatccaag tttaaaacag tatagagagg
     4381 tagaattata atggataggt taaaatatat ctcatcagag actttttatg aaggtattat
     4441 agttgacatc aaaggcggtg gagtgactat agacctaaaa ggtagacttg gacaatttaa
     4501 aatacctaat agaatgctta taactgacta cgaacttaag ataggtcaag aagttggatt
     4561 catgctaagc tatcctgagg tattaagtcc agagcctaat caagagtatg tagaaaatat
     4621 taggagagaa aaagaaaaac aagcagaaat gcaatcaaaa caagaagaaa aataataaat
     4681 aatcgtttga aaatagaagg gagaggaaat atatgagcct tacaacagta caaggacttc
     4741 aatctgaaat attcgttcca ataacacctc ctccagtttg gactcctgta actaaagaat
     4801 taaaagatat gactatagct ttagctacag cagctggtgt tcatttaaaa tctgacaaga
     4861 gatttaatct agcaggagat actacattta gagctatacc taatacagct acagttgatg
     4921 aaatgatggt atcacatggt ggatatgaca atggagatgt aaacaaagat ataaactgta
     4981 tgttccctat agatagatta catgaattag ctgcagaagg atttatcaaa ggagtagctc
     5041 ctatgcacta tgcattcatg ggtggtggag gaaaccaaca tgtcttcact gaagaaactg
     5101 gtcctgctat cgctgctaaa cttaaggaag agggagtaga cggtgtagtt atgacagctg
     5161 gctgaggtac ttgccataga actgccgtga tcgtgcagag agcaatagag gaagctggaa
     5221 tacctacaat aataatagca gctcttcctc cagtagttag acaaaacgga actcctagag
     5281 cagttgctcc attagttcca atgggtgcta atgctggtga accacataat atagaaatgc
     5341 aaacacatat attaagagat acattagagc aattagttgc aataccatct gctggtaaga
     5401 tagttccatt accatacgaa tataaagctc acgtttaaaa tgtaatttat atttgaaaat
     5461 atagaaccct tctcctacac ggttaggaga ggggttttaa gaacaaaggt ggatattatg
     5521 gaagaaaaaa taatgagaag acttgttata aaaccgtttc atatgaatga agttaatttc
     5581 ggttcaaaaa cttctataaa gaaagatgta cttacaatag atttatcaag tatagatgaa
     5641 ataaaagaaa gagaagagtt aataacagac ataaaagtag acattataaa acctggagat
     5701 tatgacagag aaattaatac tattatggac attataccaa tatcaacaaa agttttaggc
     5761 agactgggag agggtattac acatacttta acgggtgtat atgtaatgtt aactggtgca
     5821 gatgaagatg gaaatcaaat gcatgaattt ggttcttcag aaggaatttt gaaagaccaa
     5881 atggtatttg ggagatatgg aactccatca gttgaagatt atataatcca tgtagatgta
     5941 actttaaagg gaggtttacc ttttgaaaga acacttccac ttgcagcatt cagagcatgt
     6001 gatgatttta tacaagaaat aagagcgtct ttgaaaatgg aagacggaag aaatgctact
     6061 caagttcgtg aatattttga caaaattcga ccaaatgcaa aaaaggttgt catagtaaaa
     6121 caaatagcag gtcaaggtgc tatgtatgac aatcagttat tctcaaagga acctagtgga
     6181 tttgaaggag gaacatcaat tatagatatg ggaaatgtac ctatgattat atctcctaat
     6241 gaatacagag atggtgccct tagagctatg acttaagaga ggtggatatt atgggaatag
     6301 gaccttcaac aaaagaaaca tctcttcatc actttagaga cccactttta gacatagttg
     6361 aaagtgataa ggatgtagat ttacttggag taatagtagt aggaactcca gatggaaatg
     6421 aaaataaaac ctttgtggga caaagaacgg ccgcttggct ggaagccatg agagtagatg
     6481 gagctatagt ttcatcagat ggttggggaa actcacatgt tgactacgct aacacatttg
     6541 aggaaatagg aaaaagagat atacctgtag ttggagttac atttaatggg actcaagcta
     6601 agtttgttgt aagcaatcaa tatatggata ccatagttga tatgaacaaa tcaaaagaag
     6661 gtattgagac agaagtagtt ggagaaaaca acacaaatga aatagatgct aaaaaagctt
     6721 tggcattttt aaaacttaaa atgagaaaac atggataaaa aatttaagta agggaatgag
     6781 ttttgtggga atttctacat caatgaaaaa cacatcactt catatattta gagacccgtt
     6841 actagatttg atagataatg attctgacat agacttgtta ggagtggttg tagttgggac
     6901 atcagagcac aatgaatgga aaacattttt agcaactaga cttggaagat ggattgaatc
     6961 attgcgacct gatggagtga ttataacttt agatatggca ggaaaccagc atattgattt
     7021 tacaaatgct atagcagagt ttgtaaaatc agatatacct acagtaggtc ttactattat
     7081 gggagcagat ggattagtaa ttaccaatcc atatttggat aaagctacca taattgatta
     7141 taaaaagaca acaggctaca tagaaacaga agtagttgga gacaatcaca tggatgaagt
     7201 tgatgttaaa aaagcattgg ctttcctaaa attaaaaatg cgtaaagacg caaaataaac
     7261 ctaaggggga atttgatatg aaatttagca gaagtataca agctatagac tctcatacag
     7321 caggtgaagc gactagaata gttgtaggtg gaatacctaa cataaaagga aattctatgc
     7381 ctgagaaaaa agaatattta gaggaaaatc tagattattt aagaactgct ataatgttag
     7441 agccaagagg acataatgat atgtttggtt ctgtaatgac tcaaccttgt tgcccagatg
     7501 ctgactttgg aataatcttc atggatggtg gcggatacct taacatgtgt ggccatggta
     7561 caataggagc tatgacagca gctatagaaa caggtgtagt tccagcagta gagcctgtaa
     7621 ctcatgtagt tatggaagct ccagcaggaa taataagagg agacgttaca gttgtagatg
     7681 gaaaagctaa agaagtatca ttcttaaacg taccagcttt cttatataaa gaaggtgttg
     7741 aagttgactt accaggtgtt ggaactgtta aatttgacat atcatttggt ggaagcttct
     7801 ttgctataat acatgcaagt caattaggtt taaaaatcga acctcaaaat gctggtaaat
     7861 taactgaact agctatgaaa cttagagata taataaatga gaaaatagaa atacaacatc
     7921 caactttagc tcatataaaa actgtagact tagttgaaat atatgatgag ccaactcatc
     7981 cagaagctac ttacaaaaat gtagttatat ttggtcaagg tcaagttgac agatctccat
     8041 gtggaactgg aacaagtgct aaattagcta ctttacatgc taaaggtgaa ttaaaagtag
     8101 gagaaaaatt cgtatatgaa agtatattag gaactttatt caaaggtgaa atagttgaag
     8161 aaactaaagt tgcagatttc aatgctgtag tacctaaaat aactggttct gcttatataa
     8221 ctggatttaa tcattttgta atagatgaag aagacccact taaacatgga tttattctta
     8281 aataagtaat aaataaatta ggaaattaaa atagaatagt agccacttgt cggttgctat
     8341 tctatagctt ttaggctata agattcagaa tatatgaaaa aataaaaaaa taggaggatg
     8401 gattatggta gcaatcttaa aggcattata tgtagtattc gcagcaatct ttgcagttgt
     8461 gtttggtaaa gacgtagcta aagcaaacaa agaggggaaa ctagaagaac aaagtactcc
     8521 aaaagttgct gtaacaggat ttataacaga cttctttgat acattaggaa tagggtcatt
     8581 tgctccaaca atagcaatgt caaaggcttt aaagttaaat ataccagata aaaaaatgcc
     8641 tggtacatta aacgtagcac atactattcc agttgttaca gaagcattca tatttacatc
     8701 aattatccca gtagatggtg ttactttagt atctatggtt gtggcagcag cagttggttc
     8761 atatataggt gcaggaataa tagctaagat ggacgagaga aaaatacaac ttgttatggg
     8821 tgtaacttta gcattaacag caatattaat gttattaggt catccttgga ttaacgtatt
     8881 accaggtgga ggaaatgaat taggtcttac tggtgctaaa ttagtaatag gtgtaatagg
     8941 taactttata ctaggagctt taatgacagc tggtataggt ctatatgctc caggtatggc
     9001 aatggtttat ttcttaggaa tgtctgcaaa agttgcattc ccaatagtta tgggttcttg
     9061 tgcattatta atgccagttg caagtatgaa attcataaaa gaagatgcat atactaaaaa
     9121 agcatcagtt ataatagcta tatgtggttt aataggagtt ttcgtagctg catatatagt
     9181 aaaaactcta ccaatggata tattaaaagc attagtaata gtagttatag cttatacttc
     9241 tgctacaatg ataatggctg caaataaaaa caaaaagact gcttagttaa agactactta
     9301 gtgtttatta aatattacag ctaataataa agattataaa ctgctttaaa atattgaatt
     9361 aatattttag ggcagttttt tatttactaa attaatataa agcaattaac atgccaatta
     9421 tgtaatatat atagtgattt agtagattta atctaaaaaa cgttgtattt ttaaagaata
     9481 aagcataatt taaagctaaa tatttaaatt ttataatcaa taagttcaat aaatgttcat
     9541 atattaat
//
LOCUS       DAELLV010000141         9533 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.141, whole genome shotgun sequence.
ACCESSION   DAELLV010000141 DAELLV010000000
VERSION     DAELLV010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9533
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.141"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            136..579
                     /locus_tag="KPD58_002543"
     CDS             136..579
                     /locus_tag="KPD58_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0266304.1"
                     /translation="MIEIKTIEINKKSQIDLLMLADPCEEMIDKYLNKGTMYALYDNK
                     ELTCIAVVNEISKEICELKNIATYEHFQNMGYASKMIYHLLDVYSKKYSSMVVGTSES
                     GVPFYEKFGFVYSHKIKNFFVDNYPEPIFEGELQCVDMLYLSYNF"
     gene            737..907
                     /locus_tag="KPD58_002544"
     CDS             737..907
                     /locus_tag="KPD58_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266305.1"
                     /translation="MDSNNNTIKSTVKKGISFGSCLAMIISYTAWKSIPWAIFHGLMS
                     WIYVLYYWVKYA"
     gene            1280..2377
                     /locus_tag="KPD58_002545"
     CDS             1280..2377
                     /locus_tag="KPD58_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBG0266306.1"
                     /translation="MKEKLEEKSKIIKTSKEYKRTIIEEKDREWTKKLNDILSRGDFS
                     NEKLDKIKDLIPKEILTSENVGEVVTEDGYAKPEYDEVFKSLFTLNNDYDLLANFIND
                     ILKDAPYANRNIKQFTQIKRIIKVETDPTINYIGEKRPRLDILAEDEERNHINIEMQR
                     ALEEDYLERAEYYLSRVHGRKLEEGKEYKEIGKTVGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIFSSKTALYFIELPKIRKSSCIANRVLIWGKLIDNPSHIDIRILSKTDYVIK
                     RALDRLKELGSNEDYLLNLKRGAYIMNKSRNFKEEIFQEGIEKGIAKGKREEKFNIII
                     KLKNKGYNLSEICDIIDDLNKSEVEKVYNQN"
     gene            2582..3070
                     /locus_tag="KPD58_002546"
     CDS             2582..3070
                     /locus_tag="KPD58_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HBG0266307.1"
                     /translation="MKFYTIQTLEFWNNNKNNKYLSNDYSFILEDFDIPYKWMYSQMT
                     NRIYEFDDSMIWVWTKRPDLRCSGYLEKGKKGVLLEIEISEDNVLLSEFDLWHLPLMD
                     ITVELYEDEQIEKKVSWERIFDLELCSKLNEVNIEDLTLQGVTSKVPLSNVKLIKEFV
                     AK"
     gene            3156..3563
                     /locus_tag="KPD58_002547"
     CDS             3156..3563
                     /locus_tag="KPD58_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266308.1"
                     /translation="MSKQQTGIKSTLISEILGISYCIGATLVNKYFFKGSSIEYLPII
                     IWVLAMGAQWVYNKKYDDLIDEASKCILSRVNDIAIKVLFFSVAIVSIFLVTLDINVS
                     NLNIGMSLLVILFIQSLLKLILFIYFDRKGIYN"
     gene            3564..3782
                     /locus_tag="KPD58_002548"
     CDS             3564..3782
                     /locus_tag="KPD58_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0266309.1"
                     /translation="MSVLLKTKIKSYREKNLMTQGELAKSVGVRRETIVHLENGRYNP
                     SLKLAMDIAKVFDTTVEELFEFIEENNI"
     gene            3997..4158
                     /locus_tag="KPD58_002549"
     CDS             3997..4158
                     /locus_tag="KPD58_002549"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266310.1"
                     /translation="MKNQANGLKNELIIGKNQAYRLENELIIGKNQAYRLENELIIGK
                     NQAYRLKNK"
     gene            4551..4856
                     /locus_tag="KPD58_002550"
     CDS             4551..4856
                     /locus_tag="KPD58_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266311.1"
                     /translation="MPSMIMEVVGVLFIAIGLILTFIEIKGNFPSFYGFGQNSIGKNA
                     VGIIAAGIALIFFRNGIDSIDSIATYGVFLAIELVVIVAYFLIRKKFTKEDKHLRTN"
     gene            5264..5953
                     /locus_tag="KPD58_002551"
     CDS             5264..5953
                     /locus_tag="KPD58_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0266312.1"
                     /translation="MDILLVEDDKNIREMVYDFLISENYSVKTAIDGNTAIEIFKNYS
                     FDIILLDLMLPKSSGFEVLKYIRSVSVVPVIITTAKDSDTDKMLGLNLGADDYITKPF
                     SIVELLARIKANIRRATVYSSSRNSQDMVIQYKDLIINISKYVVSKNGIDLNLTHTEF
                     EIVKLLATNQGCAFSKEQLYNAIWKEPYYGNENVLNTHINRLRNKLSDGIEKNNYIKT
                     LWGIGYKMEEE"
     gene            5953..6870
                     /locus_tag="KPD58_002552"
     CDS             5953..6870
                     /locus_tag="KPD58_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0266313.1"
                     /translation="MELFLVVISIVLFALYLKERNKSKKMESDYKCINSRLRDITNDD
                     EINYILVPSDISIVKETAVDINKLLEKFYSRQINYNRSEKAILQIFTNISHDLRTPIT
                     VLKGYIEMLYLQSQKEELSSSMRATVEKMQTNSNELVNSINNLFNMAKIQSGDMILNL
                     QKSNLTQLCHEIILEFYDLLDKENFNVEVNIEDRPIYANVDTEALQRILKNLIDNAIK
                     YGSDGRFLGISLYEKEKHVFIDIEDKGSGISETEKEHIFTRAYTADRKKGNGLGLAIS
                     QGLANSMGGSIYVNSRPNVKTVFTVKLKS"
     gene            6943..7869
                     /locus_tag="KPD58_002553"
     CDS             6943..7869
                     /locus_tag="KPD58_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0266314.1"
                     /translation="MNYIIETHQLKKIYKDKAVVNAVNIHVKKGEIYGFVGPNGAGKS
                     TVMKMLLNLVKPNSGEIVMFSKKVEETDFEILKKIGTIIENPYLYENLTAKQNLDLHC
                     EYMGYYNKEHISEVLECVGLSKQSNKKVSKYSLGMKQRLAIARAILTKPELLILDEPI
                     NALDPEGIREMRELFRKLNTDYGITIFISSHILSEVEQIADTIGIIQDGKLIKEISMS
                     DIHKYQTDYVEVDVDNVELAGYLLEKEFGIKDFKITSESCIEIYDLRKDVKEISKIFI
                     QNQIGINSIGRKQSSLEDYFFQTTGTGGKETL"
     gene            7866..8546
                     /locus_tag="KPD58_002554"
     CDS             7866..8546
                     /locus_tag="KPD58_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0266315.1"
                     /translation="MIHLMKLELKKVGLKRYFLFSMLGIFLCMFFVFTGLNDKSTSIY
                     DYDVTFRTISLFFCFYYIILFAVLNVAYIINEYTNKTILILFSYPLDRKKLILSKLLL
                     ITTLMISSMIVGYICCFTFVVVLDKYFNLVIGDFHISILSYWIPGAIKSIIIFCSLGI
                     WTFVVGMIRKSIPVTLVSSIIFINLRQFILAGTNTTQDSLISVFIIVGITVIGIYYIL
                     NHKVTEID"
     gene            8644..8979
                     /locus_tag="KPD58_002555"
                     /pseudo
     CDS             8644..8979
                     /locus_tag="KPD58_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427885.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
BASE COUNT         3789 a          927 c         1512 g         3305 t
ORIGIN      
        1 tttattatta gtattaatat tctagggaat tatcactata ttttaaaaag taaaaatata
       61 tatcaaaatg aactaaataa gtactgtaag aagtttgtta aattaaggtt tgaataaagt
      121 gagggtggag aacttatgat agaaataaaa actatagaaa taaataaaaa aagtcaaatt
      181 gaccttttaa tgttagccga cccatgtgaa gaaatgattg ataaatatct caataaagga
      241 actatgtacg cattgtatga taataaagaa cttacttgta tagcagttgt aaatgaaatt
      301 tccaaagaaa tatgtgaatt aaaaaacatt gctacatatg agcattttca aaatatgggt
      361 tatgcctcaa agatgattta tcatctttta gatgtttata gtaaaaaata ttcatcaatg
      421 gttgttggta catctgaaag cggagttcct ttttatgaga agtttggatt tgtttattca
      481 cataaaatta aaaacttttt tgtagataat tatccagaac caatctttga aggagaatta
      541 caatgtgttg acatgttgta tctatcatat aacttttaga taggttaata taattaatta
      601 attcatataa ttatcggaat tataatttat aaattatgta taataagagt ataatttatt
      661 tagaagctgt tatattgtat tatattcaaa taagatatat aataacttgt ttattttgaa
      721 ttttggagga ttaattatgg attcaaataa taatactata aaatcaactg ttaaaaaggg
      781 tatttctttt ggttcttgtt tagcaatgat tatttcttat actgcatgga aatctattcc
      841 atgggctatt tttcatggct taatgagttg gatatatgta ctttattatt gggttaagta
      901 tgcatagatt aaattaataa ataagtatgt aatattgtaa agtttaagaa acaaaatgta
      961 agggagtgtc tcgaagtgaa cttttagttt ataaggcact cttttttgta ttaaataaaa
     1021 tatattttca ttatttaaac aatgaaaaag atgagtatct ctattttaat acataccctt
     1081 atattcattc atttatcaac aatagtttta aaaagaaagc tatctaaaat ctattgtaat
     1141 ctaagtgctt ctctttaaag tcttaacttt agtaatgata tataagttta taaggttctt
     1201 atttcaataa tgcaaatttt acttagaagt tcagactttg gtatgaagaa atgggtaata
     1261 ataaaaaaga ggtgttgata tgaaagaaaa attagaagaa aaatctaaga ttattaaaac
     1321 atcaaaagag tataaacgaa ctataattga agaaaaggac agagaatgga ccaaaaaatt
     1381 aaatgacata cttagtagag gtgatttttc taatgaaaaa ttagataaaa tcaaggattt
     1441 aattccaaaa gaaatattga ctagtgaaaa tgttggagaa gttgtaactg aagatgggta
     1501 tgctaagcct gagtatgatg aggtatttaa atcattgttt actttaaata atgattacga
     1561 tttacttgca aattttataa atgatatcct caaagatgct ccatatgcca ataggaatat
     1621 caaacagttc actcaaataa aaagaataat taaggttgaa accgacccaa caattaatta
     1681 cattggtgag aaaagaccta gacttgatat tttggctgaa gatgaagaac gtaaccatat
     1741 aaatattgag atgcaaagag ctcttgaaga ggattatttg gaaagagctg aatattatct
     1801 tagtcgtgta catggtagaa agttagaaga aggtaaagaa tataaagaaa ttggaaaaac
     1861 tgttggaata catatactaa atcatgtaaa atataatcac atagaagatt atgtaaattg
     1921 cctgagatta acaatggacg ggcatccaga tattttttct agtaaaactg cattatattt
     1981 tatagaactt ccaaaaattc gtaaatccag ttgtatagca aacagagttc taatatgggg
     2041 taaacttatt gataatcctt ctcatataga tattagaata ctatctaaaa cggattatgt
     2101 tattaaaaga gcacttgata gattaaaaga attaggtagt aatgaggact atttattaaa
     2161 tttaaagaga ggtgcgtata ttatgaataa aagtagaaat tttaaagaag aaatttttca
     2221 agaaggtatc gaaaaaggta ttgcaaaagg gaaaagagaa gagaaattta atatcatcat
     2281 aaaattaaaa aataaaggat acaatttgag tgaaatatgc gatattatag atgatttgaa
     2341 taaatcagag gttgaaaaag tctataatca aaactaatac tatgattgag atggcactag
     2401 ataaatacaa agcttcctct tttaaattta aattaatata aaactttgtt atttaaatat
     2461 ggtagatata aagagatata taaataatag gtacaaagag gtgtataaat aaaggagata
     2521 attcaaaata aaagtttgaa ccatctcctt cttatagaaa aacaaagggg ttgatttata
     2581 tatgaaattt tatactatac agactttaga attttggaat aacaataaaa acaataaata
     2641 tctatcaaat gattattctt ttatattaga agactttgat attccataca aatggatgta
     2701 tagtcaaatg acaaatagaa tatatgaatt tgatgatagt atgatatggg tttggacgaa
     2761 gagacctgat ttaagatgta gtggttattt agaaaaagga aaaaaaggtg ttttacttga
     2821 aatagaaata agtgaagaca atgtgttatt atcagaattt gatttatggc atttaccttt
     2881 aatggatatt actgtagagc tttatgaaga tgaacaaatt gaaaagaagg taagttggga
     2941 aagaatattt gacttagaac tgtgttcaaa attaaatgaa gttaatatag aagatttgac
     3001 attgcaagga gtgacatcta aagtgcctct atctaatgta aaattaataa aagaatttgt
     3061 tgcaaaataa tatttttatt gaaatttatt caaaaaagtt atatatttat aacaagaggt
     3121 gttataaata cataactttt taagggggat tttatatgtc taaacaacaa actggtatta
     3181 aatcaacact tatttcagaa atattgggaa tttcttattg tattggagct acattagtaa
     3241 ataagtattt ttttaaagga agtagtattg aatatcttcc tataattatt tgggtattag
     3301 ctatgggagc tcagtgggtc tataataaaa agtatgatga tttaattgat gaggcatcaa
     3361 aatgtatact atcaagagtt aatgatattg caataaaagt attgtttttt tctgtagcta
     3421 tagtatctat atttcttgta actttggaca taaatgtgtc aaatttaaat ataggaatgt
     3481 ctttattagt cattttgttt attcaatcct tgttgaagtt aattctattt atatattttg
     3541 acagaaaggg gatatacaat taggtgtcag tattactaaa gacaaaaatt aaatcctata
     3601 gagaaaagaa ccttatgaca caaggtgaac ttgcaaagtc agtaggagta agacgtgaaa
     3661 ctattgtaca tttagaaaat ggtcgctaca atccttcttt aaaactggca atggatattg
     3721 ctaaggtatt tgatacaact gtagaagagt tatttgaatt tattgaagaa aataatattt
     3781 aaatttcatg tattacttat gcattagaga atgaattaat tataggtaaa aaaccaagct
     3841 tatagattag agaataaatg acttatagat gaaaaaccaa gctaatggat taaagaatga
     3901 atgatttata gatgaaaaag aaagcttata gattagagaa taaatgattt ataggtaaaa
     3961 accaagctta tagattagag aataaatgac ttatagatga aaaaccaagc taatggatta
     4021 aagaatgaat taattatagg taaaaaccaa gcttatagat tagagaatga attaattata
     4081 ggtaaaaacc aagcttatag attagagaat gaattaatta taggtaaaaa ccaagcttat
     4141 agattaaaaa ataaatgatt tatagatgaa gaactaaact catagattaa aaaataaatg
     4201 acttatagat gaaaaatcaa gctcatagat taaagaataa ataacttatt agatgaaaaa
     4261 acaagcttat agataagaag tccatgctta taagccagaa aatgaatact ttaatagatt
     4321 aaaaaataca tatttgtgaa ttatagaatg aatgactcca taaactagaa aatttataat
     4381 aatatctata tgaaaattag aattatttta tcacaaaaaa attaagttaa ataaatatga
     4441 aattacttcc aaatatactt ccaaaaacat gtaatcgcat atataatata attataaaaa
     4501 taataaatat ttttatagta gtagttaatt attaataagg aggggtcaaa atgcctagca
     4561 tgattatgga agttgtagga gtactattta tagctattgg attaatcttg acattcattg
     4621 aaataaaagg aaatttccct tctttttatg gttttggtca aaattctata gggaaaaatg
     4681 ctgtaggtat tatagcagca ggaattgctt taattttctt tagaaatggc atagatagca
     4741 tagattctat agctacttat ggtgtatttt tagcaataga acttgttgta atagttgcat
     4801 atttcttaat aagaaaaaaa tttactaaag aagataaaca tttaagaact aattaactaa
     4861 attatctact ataaaattgg aaaattaaaa acaaataaaa gtttgttttt aattatttaa
     4921 aatccaaatt tgattatttt taaagaaact atgttctgta taaaactaga atgtggtttc
     4981 ttttttatgt ataaaatatt ttttagcaaa agttaaaagg tgttttataa tataaaatag
     5041 ttaattgtag atgttaataa aagaaaatta tgtatatttc agataaagat aagagagtat
     5101 actatatttt aattcttttt tcaataaagc ttattgaaaa gaactttaac ttagaagagt
     5161 atcaccaaat tgcagataca atatcataag cagttattta ttgacaaccc tttttaataa
     5221 atatatcatt tgaagtatac tgacgaaaag gagttgaata ataatggaca tcttattggt
     5281 tgaagatgat aagaatattc gagaaatggt atacgatttt ctgattagtg agaattactc
     5341 tgttaaaact gccattgatg gaaatacggc tattgaaata tttaaaaatt attcatttga
     5401 tattatttta ctggacttga tgttaccaaa gtctagtggt tttgaagtat taaaatatat
     5461 acgaagtgtg agtgttgttc ctgttattat tactactgcg aaagatagtg acacagataa
     5521 aatgctaggg cttaatcttg gtgcagatga ttatattaca aagccttttt caatcgtaga
     5581 attgcttgca agaattaaag ctaatattag acgtgcaaca gtgtacagca gttcaagaaa
     5641 ctctcaagat atggttattc agtataaaga tttgattata aacatatcaa aatatgtagt
     5701 ttcaaagaat ggaattgact taaacttaac acatacagaa tttgaaattg ttaaattact
     5761 agcgactaat caaggatgtg ctttttcaaa ggaacaatta tacaacgcta tttggaaaga
     5821 accttattat ggaaatgaaa atgtgctaaa tactcatatt aacaggttaa gaaataaatt
     5881 gtctgatggt atagaaaaaa ataattatat taaaactctt tggggaattg gctacaaaat
     5941 ggaggaagaa taatggagtt atttttagtt gtaatttcga ttgtactatt tgctctctac
     6001 ttaaaagaac gaaataaatc aaagaaaatg gaaagtgatt ataagtgtat caattcaaga
     6061 cttcgtgaca taacaaatga tgatgaaatt aattatattt tagtaccatc tgatatttcg
     6121 attgtaaaag aaacagcagt agatataaat aaacttcttg aaaagtttta cagcaggcaa
     6181 attaattata atagaagtga aaaagcgata ttgcaaatat ttacaaacat atcacacgat
     6241 ttacgaactc ccatcacagt attaaagggt tatattgaaa tgctatatct tcaaagtcaa
     6301 aaagaagaat tatcttcttc aatgagagca actgtggaaa aaatgcaaac aaattcaaat
     6361 gagcttgtta attcaataaa taacctgttt aatatggcaa aaatccaatc tggagatatg
     6421 atattaaatc ttcaaaaatc caatctaaca caattatgtc atgagattat tttagagttt
     6481 tatgatttgc tagataaaga aaactttaat gttgaagtta atattgaaga tagacctata
     6541 tatgcaaatg ttgacacaga agcattacaa cgtatactca aaaatttaat tgataatgct
     6601 ataaaatatg gttcagatgg tagattttta gggatttcac tatatgaaaa ggaaaaacat
     6661 gtttttatag atattgagga taaaggttca ggtatttctg aaactgaaaa agagcatatt
     6721 ttcacaagag cttatactgc tgacagaaaa aaaggcaatg gtttaggttt ggctatttct
     6781 caagggcttg caaactctat gggaggttca atttatgtaa atagtagacc taatgtaaaa
     6841 acagttttta cggtaaaatt gaaaagttag aaaaaagtta gatttaaaag agagaaaaga
     6901 caattttatt ttgtatactg tttttagaaa ggagggagta tgatgaacta tatcatagag
     6961 acccatcaat taaagaaaat atataaagat aaagctgttg ttaatgctgt taatattcat
     7021 gtgaaaaagg gagaaattta tggatttgtt ggaccaaacg gggcaggtaa aagtacagta
     7081 atgaaaatgt tgctaaatct tgttaagcca aattctggag aaatagttat gttcagtaag
     7141 aaagtggaag aaactgattt tgaaatatta aaaaagattg gtactattat tgaaaatccg
     7201 tatttatatg agaatttgac tgctaaacag aatcttgatt tacactgtga atatatgggt
     7261 tattataaca aagaacatat atcagaggta ttggaatgtg tcggattgtc aaagcaatca
     7321 aacaagaaag tttcaaaata ttctttaggg atgaaacaac gtcttgcaat agctagagca
     7381 attcttacta aaccagaatt attgattctt gatgaaccta taaatgcact cgacccagag
     7441 gggataagag aaatgcgaga attattccgt aagttaaata cagattatgg aataactata
     7501 tttatttcta gtcatattct ttcagaggta gaacaaattg cggacactat tggaattatt
     7561 caagatggta aacttattaa agaaatttct atgtcagata tacacaaata tcaaacagat
     7621 tatgttgaag tagatgtaga taatgtggag cttgctggat acttgttaga aaaagaattt
     7681 ggaataaaag actttaaaat tacttcggaa agttgtattg aaatatatga tttacgaaaa
     7741 gatgtaaaag aaatatccaa aatatttatt caaaatcaaa ttggtataaa tagcattgga
     7801 cgtaaacaaa gttctttaga agattacttc ttccaaacta caggtacagg aggtaaagaa
     7861 acactatgat acatttaatg aaactagagc taaaaaaagt tgggctaaaa agatattttt
     7921 tattttcaat gctggggatt ttcttatgta tgttttttgt ctttactgga ttgaatgata
     7981 aatctacttc aatatatgat tatgatgtta catttaggac aatcagttta tttttctgtt
     8041 tttactatat tatattattt gctgtgttga atgtggcata tataataaat gaatatacaa
     8101 ataaaacaat cttgatttta ttctcttatc cacttgaccg taaaaaactt attttatcaa
     8161 agttactttt gattacgact cttatgatat catcaatgat agttggatat atctgttgtt
     8221 ttacattcgt tgttgtgttg gataagtatt ttaatttagt tattggagat tttcatattt
     8281 caattttatc ttattggata ccaggagcaa ttaaaagtat tattatattt tgctcattag
     8341 gtatatggac attcgttgtt ggaatgataa gaaaatcaat acctgttacc cttgtcagtt
     8401 caattatttt tatcaattta agacaattta ttttagctgg tacaaataca actcaagaca
     8461 gtttgatttc tgtttttatt atagttggta taactgtgat tggtatatat tatatactaa
     8521 atcataaagt tactgaaatt gactagatac tgtggctagg aaggtagtta ttaattgtat
     8581 cttcttggga taataatgat ttgctattat atcagctatt attaatatta ttagagtttt
     8641 aataatattg cacaaccatt tgaagttgag ccaaagcttg atgaattaat tgaatggtac
     8701 tatagtcaga gtgaagtatc tattaaagtt atagctgaat ttcattatag atttgaattg
     8761 atacatccat ttcaagatgg taatggtcac ataggtagat ttgttatgct aaaacagatg
     8821 ttagaaaata atcttcctat aaaaattgta tcttgggata gtgaggactt gtatagaaac
     8881 tccttaaatt cttgtagttt aggtaattat gtccctttaa ttgaatattt atcaagttta
     8941 gaagatttta gagaagtata taaaatgtta tggaaataat tgtttgcagc aaaaaaaaat
     9001 tttatataaa tatattttta tctatgctat ttaacgctaa tttcttagtt attcttagct
     9061 tggactatat ccactatttt aactatataa aatagtggat atagtttaac tatctcttta
     9121 taactattct tatgtatctt agttgtttct taatatgggg acagttcata aaactaaaag
     9181 tgatttttaa ttttatcatt tataatttac cattacatca gaaaaatgct tatcattaga
     9241 attttcataa aagtcaccta tttttttcaa ttaactaaat caattctatt agtcttaatc
     9301 tttcgtattt caaattgata atataagaaa ataaaaaata aaaaatttta aattttttat
     9361 ttattgcgaa aaaaataaaa aggtgctata ctttttgtaa catattattc aaattattaa
     9421 gagatagtaa acatttatag tttaatttgt agtacactag catgatttgc agaacaatga
     9481 attaatctat agtataatag tttaatttaa agtacaacga tttaatctat agt
//
LOCUS       DAELLV010000142         9496 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.142, whole genome shotgun sequence.
ACCESSION   DAELLV010000142 DAELLV010000000
VERSION     DAELLV010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9496
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.142"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(90..743)
                     /locus_tag="KPD58_002556"
     CDS             complement(90..743)
                     /locus_tag="KPD58_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0266316.1"
                     /translation="MKVDPLTTQVYDYISKKIQNGEYEANQRITESEICKSIGVSRTP
                     AREALTRLAGENLLEKIPNKGFVVKEFQEKEKLDTYSVIGVLDALAGSSALQNLTESD
                     LVKMEELTEMMAVSIKYKNYNSYLKLSNEFHDIYITKSDNQVLVNLLNSLRYNFMSKS
                     YTSDNEDELYKMLTYSNNQHIEVVKSMRENDLENVERVLREHWKTIPIAEMKKEKLL"
     gene            complement(988..2853)
                     /locus_tag="KPD58_002557"
     CDS             complement(988..2853)
                     /locus_tag="KPD58_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HBG0266317.1"
                     /translation="MQSVIEYNSWDKNFKAPFGALKFDEELTICVKVNEGYNIKSISL
                     EINREEEMRTITLNEELDNDKLGKCFCGKIEKFDGTGVYFYYFKVDVEMDGQIKTLFY
                     GKNRDNGYSCEYNYSDINKYQITVYKDFKVPTWYKEGILYHIFVDRFNNGNRSGKVDN
                     PKKNSFIYGNWEDTPMYIKDSQGDVIRWDFHGGNLRGIINKLGYLKKLGVSILYLSPI
                     FEASSNHKYDTGDYKKIDPMFGDEDTFKELIDKAKEKGISIVLDGVFSHTGADSKYFN
                     MYGNYNSLGAYQSKESPYYSWYMFEEFPQKYKSWWDVKTLPNINELEHSYMDYIIYDN
                     DSVINKWVNMGIKGWRLDVADELPTKFIRELKKELKKADDDSILIGEVWEDASNKISY
                     GQRRSYLLGEELDSVMGYPFRNNMFSFLKGEINSYELCNRYMQIKENYPKESFRSNLN
                     LIGTHDVTRAKTELNDDVDLVKLAVAIQMTFEGVPYIYYGDEAGLCGGVDPDNRRTYP
                     WKNEDEDMLNFYRDVIKIRNKNKLLSSGNTEFIYTNNDSVFSFIRVNENNDRMLILVN
                     RSENVESISLCIESSFIEEIPIKYSLKNANSTIQIENNELKVDLDSKSFRIFRVN"
     gene            complement(2858..5278)
                     /locus_tag="KPD58_002558"
     CDS             complement(2858..5278)
                     /locus_tag="KPD58_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HBG0266318.1"
                     /translation="MVTISKKKFKQKFEVKMYSLYAQSIKEATDEQLLNVLCSLLKDE
                     IAKKWVATKLDKKKEVYYFSLEFLIGRQLKSNLLNLNIEEEVREGLSELGINLDDLIE
                     AEVDPALGNGGLGRLAACFLDSMASLNISGQGYGIRYKYGLFEQKFVNGYQVEVPDNW
                     LTEGRYAWETVRPNEATMVKFGGEVELIKEGSHLKVIHKNYLPVMAMPYDIPIIGYQN
                     QCINTLRLFKSEIPKRDFGELTSNALNYSGSYEEALKHKYYTEEISQVLYPDDSNYAG
                     KLLRLKQEYFFVSAGIQDIIRKYKKNKLNINNLFDKVAIHINDTHPTLCIPELMRILL
                     DEENLSWDEAWEITKKTVSYTNHTIMSEAMEKWPVSMMKELLPRIYMIIEEINRRYVE
                     ELNNKGYDQDRIKRMSIIDCDNINMANLCIVTSHSVNGVAKLHTHILETEVLKDFYQD
                     EPNKFNNKTNGIAHRRWLISSNPQLSNLITDLIGDSWKKDTLQLKNIEKFKNDSSVLQ
                     RLDDIKYNNKANLAKFIKDKYDLNVDPSSIFDVQVKRLHAYKRQLLNIFNVLHMYHEL
                     LDNPNLNLDPRTFIFGAKAAPGYYLAKCIIKFINSVASTINNDVRVKDKLKVVFLENY
                     GVSLAEIIIPAANVSEQISTTTKEASGTSNMKFMMNGAITLATLDGANVEICEQVGKE
                     NMFLFGLSAEQVLNYNKYGGYSSLDLYHSNMDIKRVVDDLINGFIPNLGEEGRSIYNS
                     LTTYNDEYFVLRDFENYGQAQADINRLYRDKEKWNKMSLVNIANSGFFSSDRTISEYA
                     KDIWFKRV"
     gene            complement(5292..6734)
                     /gene="glgA"
                     /locus_tag="KPD58_002559"
     CDS             complement(5292..6734)
                     /gene="glgA"
                     /locus_tag="KPD58_002559"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011053260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HBG0266319.1"
                     /translation="MKVFYVTAECWPFAKTGGLGDVSYALPKELKKEGVDVRVIMPKY
                     STIPSYLKDQLKEIAVFSVRVGWRNQYCGLLEMELDGVKFYFIDNEFYFRREDERKSI
                     YGYGDDAERYTFFTDAVLEAISRIDFYPDVIHINDWHTGMLPLILKERYATLEGYKNI
                     KTMYTIHNLQYQGVFDKHVLYDILDLPQKYFDNGDIEYYGSINFMKAGINFADKIITV
                     SPTYANEIQTSFYGEQLDGLLRKESGKLKGILNGIDYDLNDPAKDKDIFVHYDVDSID
                     KKVENKLRLQDILGLKKDSSIPLIGIVSRLVSQKGFDLIAYMMPELMREDLQIVVLGT
                     GEHQYQSMFNYYDSNFSDKVSARITFNASLAQQIYAASDMFLMPSLFEPCGIGQMLAM
                     RYGSLPIVRETGGLRDTVTPYNKFTGEGNGFSFKNYNAHEMFFCLKNAIKVFKDKEKW
                     IKLVENAMKTDNSWKKSAKEYIETYRDICD"
     gene            complement(6763..7893)
                     /gene="glgD"
                     /locus_tag="KPD58_002560"
     CDS             complement(6763..7893)
                     /gene="glgD"
                     /locus_tag="KPD58_002560"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HBG0266320.1"
                     /translation="MRNECLGIINLNKKGDPAINKLNYGRPIASTPIAGRYRIIDFAL
                     SNMINSGITKVGIFAKEKYRSLTDHIGSGKDWDLSRKKGGLSIFSPENTKYRNTYSHR
                     EGDIYTILANLDYIEKSEEEYILIAPSYMLCNLNYSQALEYHKKSHNDITIIYKNVNN
                     ANKDFAGNLTLNLDSNNRVINVGNNLGKFPRANICMETYIMKREDFVECIYNIVNKGN
                     YCYLEEFIIEEAENMKIGAYEYTGYLKCVNSVESYFEMSKDLLNLEVADELLYSERKI
                     FTKEKNESPTIYTDSAKVENSFIASGCLIEGTVKDSIIFRKVNVEKGTVIENSIVMQN
                     CVIKSNAKLYNAILDKNTSVSNGKELRGDEKYPLVVEKNTNI"
     gene            complement(7895..9055)
                     /locus_tag="KPD58_002561"
     CDS             complement(7895..9055)
                     /locus_tag="KPD58_002561"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HBG0266321.1"
                     /translation="MKKEMLAMILAGGQGSRLGVFTKRIAKPAVSFGGKYRIIDFVLS
                     NCSNSGIDTVGVLTQYRPLILNSHIGMGSHWDLDRINGGVYVLQPFMNEKEGNWYNGT
                     AHAIYQNMDFVDTYNPEYVLILSGDHIYKMDYSKMLKFHKEKGSKATIAVIEVPWDEA
                     SRFGIMNTNEDSSIYEFEEKPSEPKSNLASMGVYIFDWKMLRNYFKEAEKNPEINYDD
                     FGKNLIPKMLEDNVGMYAYPFKGYWRDVGTIQSLWDANMDIIKSPETLDLADPKWKIY
                     TNTMAMPPQYIGKNANVHRSMIADGCRILGEVGNSVLSHGVVVGKGSKVIDSVIMPNV
                     VIGENVTIEKAMIGECATINDNVQIKNVNNEINVVSEYENIEPRCVLIEGGL"
BASE COUNT         3015 a         1729 c          919 g         3833 t
ORIGIN      
        1 tatttgtatt taaagttaaa tttattcttg tttttccttt aatatttaat agtaaaacaa
       61 caaaattata aaaaaaataa agagaaactt tataaaagct tctctttttt catctctgct
      121 ataggtatcg ttttccaatg ttctctcaaa actctttcaa cattttctaa atcattctcc
      181 ctcatagatt ttactacttc aatatgttga ttattgctgt atgtcaacat tttatataac
      241 tcatcttcat tatcactagt atatgacttt gacataaaat tatatcttaa agagttcaat
      301 agatttacta acacctgatt atctgattta gttatgtata tatcgtgaaa ttcattactt
      361 aattttaaat agctattgta atttttatac tttatagaga ccgccatcat ttccgttaat
      421 tcttccattt ttactaggtc tgattcagtt aaattttgta atgctgaaga accggctaac
      481 gcatccaata ctccaattac ggaataggtg tctaatttct ccttttcctg aaattctttt
      541 actacaaatc ccttattagg tattttttct agtaaatttt cacctgctaa cctagttaat
      601 gcttctctcg ctggtgttct actaactccg atagatttac aaatctcaga ttcagtaatt
      661 ctctgattag cttcatactc tccattttgg attttctttg atatatagtc atatacttga
      721 gttgtaagtg gatctacctt cataagacgt tcctccatat taagattata aaagttaaat
      781 ctccctacaa ctttggtaga gagttcagtt ttatcttact aatttatata atatcacaat
      841 tatgatattt tttatacttc tttgtaatat tttgaaatac taaaattctt atgataattt
      901 atacaaaaaa agtagaaaaa gttttgataa aacaaaactc tttccacatt caccttattt
      961 tctatttatt tttctaaaaa aagatattta atttactcta aaaattctaa atgatttaga
     1021 gtctaaatct actttcaact cattattttc aatttgtata gtactatttg catttttaag
     1081 actatacttt ataggtattt cctcaataaa actactctct atacataatg atatactttc
     1141 cacattttca cttctattta ctaaaattaa cattctatca ttattttcat taactctaat
     1201 aaaagaaaat acagaatcat tattagtata tataaattct gtatttccag aacttaaaag
     1261 tttattctta tttctaattt ttataacatc tctataaaaa ttaagcatat cttcgtcttc
     1321 atttttccat ggataagttc ttctattatc tgggtcaaca cctccacaaa gacctgcttc
     1381 atctccataa tatatatatg gtactccttc aaatgtcatc tgtatagcta cagcaagctt
     1441 cactaaatct acatcatcat ttaattccgt tttagctctt gtaacatcat gtgttccaat
     1501 taaatttaaa ttagacctaa atgattcttt tggataattt tctttaatct gcatatatct
     1561 attacaaagt tcatatgaat taatttctcc ttttaagaaa gaaaacatgt tatttctaaa
     1621 aggataaccc ataacagaat ctaattcttc acctaataaa taacttcttc tctgaccata
     1681 gctaatctta tttgatgcat cttcccatac ttcacctata agaattgagt catcatctgc
     1741 cttttttaat tcctttttta actctcttat aaacttagtt ggtaattcgt ctgcaacatc
     1801 tagtctccag cccttaatac ccatatttac ccatttattt ataacgctat cattgtcata
     1861 tattatataa tccatatagc tatgttctaa ttcatttata tttggcaatg tcttaacatc
     1921 ccaccaactt ttatattttt gaggaaattc ttcaaacata taccatgaat aatatggaga
     1981 ttctttagat tgatatgccc ctaaactatt atagtttcca tacatattaa aatatttact
     2041 atctgctcca gtatgactaa aaactccatc taatactatt gatatacctt tttcttttgc
     2101 tttatcaata agttctttaa atgtatcttc atctccaaac attgggtcaa tttttttata
     2161 gtcacctgta tcatacttat gattactaga ggcttcaaat atagggctta gatatagtat
     2221 acttacacct aattttttta aatatccaag tttattgatt atccctctta aatttccacc
     2281 atggaaatcc catcttataa catccccttg actatccttt atgtacatag gtgtatcttc
     2341 ccaatttcca tatatgaaac tatttttttt aggattatca accttaccac tacgatttcc
     2401 attattgaat ctatctacaa atatatgata aagtattcct tctttgtacc atgttggtac
     2461 tttaaaatct ttgtaaacag tgatttgata tttattaata tcactataat tatattcaca
     2521 agaatatcca ttatctctgt tttttccata aaataaagtc tttatctgac catccatttc
     2581 aacatcaact ttaaagtaat aaaaataaac acctgtacca tcaaactttt caatcttacc
     2641 acaaaagcac tttccaagtt tatcattatc taattcttca tttaaagtta ttgttctcat
     2701 ttcttcttct ctatttattt ctagggaaat agacttgata ttgtatccct catttacttt
     2761 aacacaaatg gtcaattcct catcaaattt taaagctcca aacggagcct taaaattttt
     2821 atcccaagag ttatattcta ttacactttg catttaatca caccctttta aaccaaatgt
     2881 cttttgcata ctcagaaatt gttctatcag aagaaaaaaa tccagagtta gctatattaa
     2941 ctaaagacat tttattccat ttttctttat ctctatataa cctgtttata tctgcttgag
     3001 cttgaccata attttcaaaa tctctcaata caaaatactc atcattgtaa gtagtgagtg
     3061 aattataaat acttcttcct tcttcgccaa gattaggaat aaatccattt attaaatcat
     3121 ctactaccct ttttatatcc atattagaat ggtataggtc aagtgaagaa tatccaccat
     3181 atttattata atttagtact tgttctgcac taagtccaaa taaaaacata ttttctttac
     3241 caacctgttc acatatctct acatttgcac cgtcaagtgt agccaaagtt atagcaccat
     3301 tcatcataaa cttcatatta cttgttccag atgcttcctt agttgtagtt gaaatttgct
     3361 cactaacatt tgctgctggt attattattt ctgctaatga tactccataa ttctctaaaa
     3421 atactacttt taacttgtcc tttactctaa catcattatt tatagtactt gcaacagaat
     3481 ttataaattt aattatacat ttagccaaat aatatcctgg tgctgctttt gcgccaaata
     3541 taaaagttct tgggtcaaga ttcaaattag gattatcaag taattcatga tacatatgaa
     3601 gtacattaaa tatattcagt aactgtcttt tatacgcatg caatctctta acttgaacat
     3661 caaatatcga acttgggtct acatttaaat cgtatttatc ttttataaac tttgctagat
     3721 ttgctttatt attgtatttt atatcatcaa gtctttgtaa aacagaggaa tcatttttaa
     3781 atttttcaat attttttaat tgtaatgtat ctttcttcca tgaatcacca attaaatctg
     3841 taattaaatt gctaagttga ggatttgaac ttataagcca tcgcctatgt gcaattccat
     3901 tagtcttatt attaaattta tttggttcat cttgatagaa atcttttaat acctctgttt
     3961 caagaatatg agtatgaagc ttagcaactc catttacact atgacttgtg acaatacata
     4021 agtttgccat attgatattg tcacagtcta ttatactcat tctttttatt ctatcttggt
     4081 catatccttt attatttaat tcctctacat atcttctatt tatttcttct attatcatat
     4141 aaattcttgg taatagttct ttcatcatac ttacaggcca tttttccatt gcttctgaca
     4201 ttatagtatg attagtatag gacactgttt ttttagttat ttcccatgct tcatcccaag
     4261 ataaattttc ttcatcaagc aaaattctca taagttctgg tatgcataag gtaggatgtg
     4321 tatcatttat atgaattgct accttatcga ataaattatt aatatttaat ttgttctttt
     4381 tatattttct tattatatct tgtatccctg cacttacaaa aaagtattct tgtttcaacc
     4441 tcaacaactt acctgcatag tttgaatcat caggatataa tacctgtgat atttcctcag
     4501 tataatactt atgttttaaa gcctcttcat agctaccaga ataatttaga gcatttgagg
     4561 taagttcacc aaaatctctc tttggtattt cactcttaaa caatcttaaa gtatttatac
     4621 attggttttg gtatccgatt attggtatat cataaggcat tgccattaca ggaaggtagt
     4681 ttttatgaat tacttttaaa tgactacctt ccttgattaa ttctacctca ccaccaaact
     4741 tcaccattgt tgcttcattt ggtcttacag tttcccatgc atatcgtcct tctgtaagcc
     4801 agttatcagg cacttctacc tgataaccat ttacaaattt ttgttcaaac agaccatatt
     4861 tataccttat tccatatcct tgaccactta tgttcaaaga tgccatagaa tctaaaaagc
     4921 aagctgctaa tctaccaagt ccaccatttc ctaaggctgg gtctacttca gcttcaatta
     4981 aatcatccaa gttgattcct aattcagata aaccttctct aacttcttct tcaatattta
     5041 aatttaataa attagatttt aactgtctcc ctattaaaaa ttctaaacta aaataataaa
     5101 cttctttttt cttatctaac tttgtagcaa cccatttttt tgctatctca tctttcaata
     5161 gactacataa tacatttaac aattgttcat cagtagcttc cttgatagat tgtgcatata
     5221 agctatacat ttttacttca aatttttgct tgaatttctt tttgcttatt gttaccaaaa
     5281 aaaccactcc tttaatcaca tatgtctcta tatgtttcta tatactcctt agctgatttt
     5341 ttccaactat tatctgtttt cattgcattt tcaactaatt taatccattt ttctttgtcc
     5401 ttaaatactt tgattgcatt tttcaaacag aaaaacatct catgagcatt gtaattttta
     5461 aaactaaatc cattgccttc tccagtaaat ttattataag gtgttactgt atctctaagc
     5521 cctccagtct ctctaactat tggtaaagaa ccatatctca tagctaacat ttgacctata
     5581 ccacaaggct caaataatga tggcattaag aacatatcac ttgcagcata tatctgttga
     5641 gccaatgatg cattaaaagt aattctagct gatactttat ctgaaaaatt agaatcgtaa
     5701 tagttgaaca ttgattgata ttgatgttct ccagttccta aaacgacaat ctgtaaatct
     5761 tctctcataa gttctggcat catgtatgct attaaatcaa atcctttttg agaaactaat
     5821 ctcgaaacta tacctatcaa cggaatagat gaatcttttt taagtcccaa tatatcttgt
     5881 aatctcaatt tattttctac ttttttgtca attgagtcta catcataatg aacaaatata
     5941 tctttatctt ttgcagggtc atttaaatca taatctatac catttaatat accttttaat
     6001 tttcctgact cttttctaag taatccatct aattgttcac cataaaaaga tgtttgtatt
     6061 tcatttgcat aagtaggact aacagttata attttatctg caaaatttat accagctttc
     6121 ataaaattta ttgagccata atactctata tctccattat caaagtattt ttgtggtaaa
     6181 tccaatatat catataaaac atgcttatca aatacacctt gatattggag attatgtata
     6241 gtatacatag tttttatatt tttatacccc tcaagggttg catatctttc ttttagaatt
     6301 aatggtaaca tccctgtatg ccaatcattt atatgtatta catctggata aaaatcaatt
     6361 ctacttatag cttctaaaac tgcatctgta aagaaagtat atctttctgc atcatctcca
     6421 tatccataga tactctttct ttcatcttct cttctaaaat aaaattcatt atctataaag
     6481 taaaatttta caccatccaa ttccatttct aaaagaccac agtattgatt tctccatcca
     6541 acacgtacac taaacacagc tatttctttt agttgatctt ttaaataact tggtatagtt
     6601 gaatatttgg gcattattac ccttacatct acaccttctt tttttaattc tttaggcaat
     6661 gcataagaaa catctcctaa tcctccagtt tttgcaaagg gccaacactc tgctgttaca
     6721 taaaaaacct tcattagtca ttcccccttc taaaaaattt atttatatat ttgtattctt
     6781 ttccactaca agaggatatt tttcatctcc tcttaattct ttcccatttg atacactcgt
     6841 atttttatct aatattgcat tatatagttt tgcatttgac tttattacac aattttgcat
     6901 tactattgag ttctctataa ctgttccttt ttcaacattt acctttctaa atatgatact
     6961 atcttttaca gtaccttcta ttaaacaacc agatgctata aagctatttt ctactttagc
     7021 actatctgta taaattgtag gtgattcatt cttttctttt gtaaatatct ttctttcaga
     7081 atataataat tcatctgcaa cttctaaatt taataaatct ttgctcattt caaaatatga
     7141 ttcaacagaa tttacacatt ttagatatcc tgtatattca taggctccta ttttcatgtt
     7201 ttctgcttct tcaataataa attcttctaa ataacaataa ttgcctttat ttacaatgtt
     7261 atatatacat tcaacaaaat cttcacgctt cataatatat gtctccatac atatgtttgc
     7321 tctagggaac ttacctaaat tatttcctac atttattacc ctattgttac tatctaaatt
     7381 tagagttaag ttaccagcaa aatctttatt tgcattattt acatttttat atattatagt
     7441 tatatcatta tgagattttt tatggtattc caatgcttga gaataattta aattgcatag
     7501 catatagctt ggagctatta aaatatattc ttcttcactc ttttctatat aatctagatt
     7561 agctaagata gtatatatat caccttctct atgtgagtaa gtatttctat acttagtgtt
     7621 ttctggacta aaaattgata ggccaccttt ttttctactt aaatcccaat ctttaccact
     7681 acctatatgg tctgttaatg atctgtattt ttcttttgcg aatattccaa ctttagtgat
     7741 tcctgaattt atcatattag ataaagcaaa gtctattatt ctatatcttc cagctattgg
     7801 tgtagatgct attggtcttc catagttaag tttgtttatt gctgggtctc cttttttgtt
     7861 taaatttatt atccctaaac attcatttct catcttaaag acctccttct attaatacgc
     7921 atctaggttc gatattttca tactcagaaa ctacatttat ttcattattt acattcttta
     7981 tctgaacatt atcatttata gttgcacact cacctatcat agctttttct atagttacat
     8041 tttcacctat tacaacattt ggcattatta ctgaatcaat tactttactt cctttaccaa
     8101 caacaactcc atgtgataat acagagttac ctacttcacc taaaattcta caaccatcag
     8161 ctatcattga tctatgaaca tttgcatttt tacctatata ttgtggaggc attgccatag
     8221 tatttgtata tatcttccac ttagggtctg ctaaatcaag tgtttctgga gattttatta
     8281 tatccatatt agcatcccat agactttgta tagttcctac atctctccag tatcctttaa
     8341 atggataagc atacattcca acattatctt ctaacatttt aggtattaaa ttttttccaa
     8401 aatcatcata atttatttct ggattttttt ctgcttcctt aaaataattt ctcaacattt
     8461 tccaatcaaa tatgtatact cccatagatg ccaaattgct cttaggttca cttggttttt
     8521 cttcaaattc atagatactt gaatcttcat ttgtattcat tatcccaaat cttgaagctt
     8581 catcccaagg aacttcaatc actgctattg tagctttaga gcctttttcc ttatgaaatt
     8641 ttaacatttt gctataatcc atcttgtata tatggtcacc agataatatc aatacatatt
     8701 ctggattata ggtatctaca aagtccatat tttgatatat cgcatgtgct gtaccattat
     8761 accaatttcc ttctttttca ttcataaatg gttgtaaaac atagacacca ccatttattc
     8821 tgtccaaatc ccaatgactt cccatgccta tatgtgaatt taatatcaac ggtctatatt
     8881 gagttaaaac acctacagta tcaataccag agtttgaaca attgcttaac acaaaatcta
     8941 taattctata cttacctcca aatgatacag ctggtttggc aattcttttt gtaaaaacac
     9001 ctaatcttga accttgccct cctgccaaaa tcatagctaa catctctttt ttcatttaat
     9061 gacactcccc tcatctagta attaatattt ataattgcat ttttactttt ataaacttta
     9121 tcagtcaata aatgtcgtgt ataaatgttg cttaaataat accttaataa aaataaaata
     9181 atatcaacta tacaattgat ataatgtata ccccataatg gacttaccga cgtatacatt
     9241 attttccata agcactatag tatttattaa tattcaatta tcggttaaaa aataatacaa
     9301 attttatttt ataaatataa aaacattttt tataaatctt ttgtgtcata taaacatgct
     9361 tattttttaa tttgttaaat tcgtaatagc aaaatataat ctaatatttg ttaaataatt
     9421 atccaaagaa aatcaagtaa ttattaatca actttatttt ttaattgata cttctttgtc
     9481 tataatttaa acaaaa
//
LOCUS       DAELLV010000143         9471 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.143, whole genome shotgun sequence.
ACCESSION   DAELLV010000143 DAELLV010000000
VERSION     DAELLV010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9471
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.143"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(64..336)
                     /locus_tag="KPD58_002562"
     CDS             complement(64..336)
                     /locus_tag="KPD58_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266322.1"
                     /translation="MYDEIIEIYFDYWINEHMYRDILEKDKYYIESWKEVKKINELLE
                     KVLEDDKKYVLVELNDLFNSLIEFERELSYKLGFKTGAKFIIELMD"
     gene            complement(584..1918)
                     /locus_tag="KPD58_002563"
     CDS             complement(584..1918)
                     /locus_tag="KPD58_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0266323.1"
                     /translation="MNNSNNLIKNTEAEQSILGSILLDSTIVQELEQMLNEDMFYHEE
                     HKYIFNAIKEINKRNEVIDIITLTEELKRKGHLNNIGGISYITSLSTIVPTVSNFMSY
                     VSIVIEKYKNRNIIDKFKKFNLGEIDDNTLIYQLENDINKANQKLIKKDSSITAISER
                     AFEYINDEVNEGIKTGIKFLDDTIGGLYGGELTTIAAKSGRGKTALALQILRNVIFQG
                     KKVLFISGEMSDIQILFRNIASLTGISVVKMKNRNLNDEEIVKYIQALSIINQENNLY
                     INDSVNTISGIKREIKSIKPSLVIIDYLQILDYEGKEVGEAKFADLSRQIKKITLDFN
                     IPVIQLAQLNDELKDERPKGDRVMRSSKQIYMDSNSVIYIHRPTEKEALNYCKTMGIE
                     DIRELRRREKGFGANLSEIIVAKNRDGESGFRPYWYIGNLFSFEECKKIQEI"
     gene            complement(1931..2674)
                     /locus_tag="KPD58_002564"
     CDS             complement(1931..2674)
                     /locus_tag="KPD58_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266324.1"
                     /translation="MSNKGWIKLYRELLDKSIWKDIKPERKVILITLLLMASHKENHV
                     FIDNKRIKIKSGQFISSYSKIAERCGKGVSYQMVRSCLLVFKNAGFLTYETTNKYIVI
                     TIENWELYQENIENQQPNQQTINNQSTTIKNIKNENNIIYSEIISKFNSTCIELSKVK
                     KLTDTRKRRINSRIKEYDKETVLEVINIVSKTSFLNGNNNTGWKANFDWIMNSSNFTK
                     ILEGNYNSKEIVINKETGIYNPDDSQLKW"
     gene            complement(2696..3010)
                     /locus_tag="KPD58_002565"
     CDS             complement(2696..3010)
                     /locus_tag="KPD58_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266325.1"
                     /translation="MKENNNLEVLKTLQATLNIIVKNEEEAQEKKQGYKIIENAKRNK
                     AELITCKEASKLYPIGESKFRQLCHSKNKGFPCIWINNRVYIIKNKLDDWFIENANGI
                     KL"
     gene            complement(3014..3214)
                     /locus_tag="KPD58_002566"
     CDS             complement(3014..3214)
                     /locus_tag="KPD58_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0266326.1"
                     /translation="MGINLKIFRIKKGLKQQELADRVGISRYYLSNLETGKANNPSND
                     LMIKLSRALDTTVEELFFSKED"
     gene            3361..4125
                     /locus_tag="KPD58_002567"
     CDS             3361..4125
                     /locus_tag="KPD58_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0266327.1"
                     /translation="MQIGKNLKNIRKQKELTQIQLAEISGISRNALINYENDKRIPSI
                     DTLSKLAKALKIEKTIFYFDIENEVMTNEDVLYLLQSLFPEEYYSFDEFLNAISNLME
                     DDDIRITSSLLLGAWSDKDQYYKLGEKLELNENQVYNWILSDCISNTELDFSLHPDDI
                     KEIIKNDVLTKQSIDELLDIGLSEENIETIYDYFSDSTDNGSNIRLKKLISQKENITI
                     KNNDYFKLLQENNELKEKNEKLENTLKEIKKLLTKI"
     gene            4182..4380
                     /locus_tag="KPD58_002568"
                     /pseudo
     CDS             4182..4380
                     /locus_tag="KPD58_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729178.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4377..4868
                     /locus_tag="KPD58_002569"
     CDS             4377..4868
                     /locus_tag="KPD58_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0266328.1"
                     /translation="MMNNTIGEKIKQYRKSINMSRDELANKIGCSVHAIAKYEQNQRM
                     PSLDMIRKIATALSIAINKLIVEENDLDTLTNNNINLKVFDKELDLVIHILENYGYDI
                     KTDYKKKFEITDNADTIKPINLNFSDFKDFSKNLNWVINGLVENFIQENSNGLIEDED
                     EEY"
     gene            4960..5712
                     /locus_tag="KPD58_002570"
     CDS             4960..5712
                     /locus_tag="KPD58_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBG0266329.1"
                     /translation="MNKSYIPKYTHKFPTNLPHGDKKYTLTYIRRMISEFVYDMGNLE
                     NNPFTFPEVQTLLDGITVGGHKLSDQNQILNIKSSWDYVIKLSNKENLSLNKDTICSI
                     HKIVAKDEALIVGDFRNGNIGIAGTTQYECIEATLLNDLFVNDINIINKITNPLEKAI
                     IINLWLSYCQFFYDGNKRTARLTSNLILISNDIGVLSIPAKHKQEYNTLMLNFYETLE
                     ADEVIKFLLEKCITFFDGYNYKSYKELFKNGN"
     gene            5702..6868
                     /locus_tag="KPD58_002571"
     CDS             5702..6868
                     /locus_tag="KPD58_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG0266330.1"
                     /translation="MATKRANGEGSIGKYKDGWRSRIMIGYNEDGKPIRKEFYGKTQK
                     EVKDKLDAYKKQYYLSSDISDDKITLEHWFYNWLFEYRIKDLKPKSFERYESIYRNYI
                     KDTDLGNVKLKDLRVSHIQKYYNRLLDSNKSIPTIRQINTKLKTCLSEAEKQGLIQRN
                     YCTMVNIPVEKKENKLEILTLEQQKSFIKAIDGHKLEVLFLLALGTGLRLGELLGLKW
                     FDIDFKKSNLTVKRTLQRTYFIDKTGNRELKVLEQEPKTSNSYRTVPIPKDVLNKLKE
                     HKVNQNSDILKAGELYRNDNYVFCNELGIPIDDKRPLRNLKSILNSLDIEPIKFHGLR
                     KTYATRLFENDVPPKTVQVLMGHYDISITLNIYTQVMEDKKVEAVEKLDKIFSL"
     gene            complement(6984..7214)
                     /locus_tag="KPD58_002572"
     CDS             complement(6984..7214)
                     /locus_tag="KPD58_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266331.1"
                     /translation="MEANKTLLQILYKKIILEFSKQTGKSLEESMDYLYTSETYKLIS
                     EGVADMHCKGHIYLAQELMLENGLMYHKGYPR"
     gene            complement(7220..7699)
                     /locus_tag="KPD58_002573"
     CDS             complement(7220..7699)
                     /locus_tag="KPD58_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
                     /protein_id="HBG0266332.1"
                     /translation="MIVYHGSYCVVKTPSITFSRDSLDFGKGFYVTSIKTQAINWTER
                     FKKRGKKAYLNSYSLDIDKLKENCKVKIFNSYNLEWLDFIIECRNMSDIYMKYDIIIG
                     GIADDKIYNTIELYQDNLIEKDEALKRLKYYKPNEQICITNQNVIDKYLKFRYEEEV"
     gene            complement(7699..7914)
                     /locus_tag="KPD58_002574"
     CDS             complement(7699..7914)
                     /locus_tag="KPD58_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3791 domain-containing protein"
                     /protein_id="HBG0266333.1"
                     /translation="MDERKLEFSIFCIEALAEKLNIDSEGVYKLLKHDTDILDNYIIP
                     CYEALHSQSKGYIVEDLMQVLKEKGVI"
     gene            complement(8230..8579)
                     /gene="ssrA"
                     /locus_tag="KPD58_002575"
     tmRNA           complement(8230..8579)
                     /gene="ssrA"
                     /locus_tag="KPD58_002575"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(8737..9192)
                     /gene="smpB"
                     /locus_tag="KPD58_002576"
     CDS             complement(8737..9192)
                     /gene="smpB"
                     /locus_tag="KPD58_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBG0266334.1"
                     /translation="MAGKKILATNKRARHEYFIEETYECGIELKGTEVKSIRQGKVNL
                     SDGFASVDNSEVFLKQVHISPYEQGNIFNVDSLRVRKLLLHKHEIRKLIGATTIKGYS
                     LIPLSLYLKNGKVKVEIALAKGKKLYDKRQDMAKKDAQRRIEREMTGKY"
BASE COUNT         3365 a         1447 c         1071 g         3588 t
ORIGIN      
        1 aaggacactt ttaatgtcct ctttaattta gcattataga attaacttat aagcataagt
       61 aaattaatcc attaattcta ttataaattt tgctcctgtt ttaaatccta atttataact
      121 aagttccctt tcaaactcta ttaatgaatt aaacaaatca tttaattcaa ccaacacata
      181 tttcttatca tcctccaaaa ctttttctaa taactcatta atttttttaa cctctttcca
      241 actttcaata taatatttgt ctttttctaa aatatctcta tacatatgtt catttatcca
      301 ataatcaaaa tatatttcta taatttcatc atacatttta gtacctcctt gactttcctc
      361 aggcacttaa tatatactta agttgctagg ttagtgtaca tcaagtacct aatttataaa
      421 ggtagttgtt atatttatat ggcaactacc tttttctaat aagtgtttaa atctaaacta
      481 aatatatttt aaatctttat tacatctaat taaaatttat atttatactt caacacttct
      541 tgaaacataa tttatctatt acttaataaa tcatgcttct tttttatatc tcttgtatct
      601 tcttacattc ttcaaaagaa aacaaatttc ctatatacca atatggtcta aatcctgact
      661 cgccatctct atttttagca acaataatct cacttaaatt agcaccaaaa cctttttctc
      721 ttcgtctcaa ctctcttata tcctcaattc ccattgtctt acaataatta agagcttctt
      781 tttctgtagg tctatgaata tatataactg aattagaatc catatatatc tgctttgagc
      841 ttctcatcac tctatcacct ttaggtcttt catctttcaa ttcatcattt aattgagcaa
      901 gctgaataac tggtatatta aaatctaaag taatcttttt tatttgcctt gataagtcag
      961 caaatttagc ttctcctact tctttacctt cataatccaa aatttgcaaa taatcaatta
     1021 tgactaagct aggcttaata ctctttattt ctctctttat tcctgatatt gtatttacag
     1081 agtcatttat ataaagatta ttttcctgat ttattatgct taaagcttgt atatatttga
     1141 ctatttcttc atcatttaga tttctatttt tcatctttac aactgatatt ccagttagtg
     1201 atgctatatt tctaaataga atctgtatat cgctcatttc tccacttata aaaagtactt
     1261 tcttaccttg aaatattaca tttctaagta tttgtaacgc caatgctgtt ttacctcttc
     1321 cacttttagc tgctattgta gttagttcac ccccatataa acctcctata gtatcatcta
     1381 agaactttat tccagtttta ataccttcat ttacttcatc atttatatat tcaaaggctc
     1441 tctctgatat ggctgttata cttgaatcct tttttattaa tttctgatta gctttattta
     1501 tatcattttc taactgatat ataagagtat tatcatctat ttctccaaga ttgaactttt
     1561 tgaatttatc aattatattt ctatttttgt atttttcaat aactatactt acataactca
     1621 tgaaatttga tactgttgga actattgttg acaaactagt gatatagctt atacctccaa
     1681 tattattcaa atgacctttt ctctttaatt cttctgtaag agttattata tctattactt
     1741 catttctctt atttatttct tttatagcat taaatatgta tttatgttcc tcatgataaa
     1801 acatgtcttc atttaacatt tgctctaact cttgtactat tgttgaatct aataaaatac
     1861 ttcctaatat tgattgctct gcttctgtat ttttaattaa gttgtttgaa ttatttataa
     1921 tataacctcc ttaccacttt aattgtgagt catctggatt atatattcct gtctctttat
     1981 ttatgacaat ttccttgcta ttataatttc cctctagtat cttagtaaaa ttacttgaat
     2041 tcattatcca atcaaaattt gctttccatc ctgtattatt atttccattc agaaaacttg
     2101 ttttactaac tatatttata acctctaata cagtttcttt atcatattct tttattcttg
     2161 aatttattct cctctttctt gtatctgtta gtttcttgac ctttgaaagt tcaatacaag
     2221 ttgagttaaa tttgcttatg atttcactat atataatatt attttcattc tttatattct
     2281 tgattgttgt tgattgattg ttaatagtct gttgattagg ttgttgattt tctatgttct
     2341 cttgataaag ctcccagttt tcaatagtta tgactatata cttgtttgtt gtttcgtatg
     2401 ttagaaaacc agcattttta aaaaccaaca aacaacttct aaccatttga taactaactc
     2461 ctttaccaca tctttcagct attttgctat aggaagaaat aaattgtcca gattttattt
     2521 ttatcctttt attatctata aaaacatggt tttctttatg actagccata agtaatagag
     2581 ttattaatat tacttttctt tctggtttaa tgtctttcca aattgattta tccaacaact
     2641 ccctataaag ctttatccac cctttattgc tcaattacaa acacctactt taaggctata
     2701 acttgatacc atttgcattt tcaataaacc aatcatctaa tttatttttt attatgtata
     2761 ctcgattatt tatccatata caagggaatc ctttgttttt agaatgacat aactgcctaa
     2821 attttgattc tcctatagga taaagcttag atgcttcttt acatgttata agttcagctt
     2881 tatttctttt cgcattttca ataattttat atccttgctt tttttcttgt gcttcttctt
     2941 cattctttac aataatgttt aaagtggctt gtaatgtctt taaaacctct aaattattat
     3001 tctctttcat attttaatcc tccttactaa aaaataattc ttctactgtt gtatctaaag
     3061 ctctagatag tttaatcatc aaatcattgc ttggattatt agccttacct gtttccaagt
     3121 ttgataaata atatcttgat ataccaactc tatctgccag ttcttgttgc tttagacctt
     3181 ttttaatcct aaatattttt aaattgattc ccatactttt tcctcctttt ttctagttta
     3241 cattcgtaaa ctttacattt taattataat gtcgacatat gtgtactgta aataaaaata
     3301 tattattttt ctgttctcat ttgtgtactt ttgggctata ctttatacga ggtgataata
     3361 ttgcaaattg gaaaaaactt aaaaaatata agaaaacaaa aagaactaac gcagattcaa
     3421 cttgctgaaa ttagtggcat ctcaagaaat gctctaataa attatgaaaa tgataagaga
     3481 atacctagta tagatacctt atcaaaactc gctaaagctt tgaaaattga aaagactatc
     3541 ttttattttg atattgaaaa tgaagtaatg actaatgaag atgttttata ccttttacag
     3601 tctttattcc cagaagaata ctattctttt gatgaatttt taaatgctat ttcaaatctt
     3661 atggaagatg atgatattag aattacctca agtttattgc taggtgcctg gtctgataaa
     3721 gaccaatatt ataaacttgg tgaaaaatta gaactaaatg aaaatcaagt ttataattgg
     3781 atactttcag attgtatatc taatactgaa ttagattttt cattacatcc tgatgacata
     3841 aaagaaatta ttaaaaatga tgttcttaca aaacaatcaa ttgatgaatt attagatatt
     3901 ggtctatcag aagaaaatat agaaacaatc tatgattact ttagtgactc aacagataat
     3961 ggaagtaata ttcgactaaa aaaattaata tctcaaaaag aaaatattac tattaaaaat
     4021 aatgattact ttaaattatt acaagaaaat aacgaactaa aggaaaaaaa tgaaaagctt
     4081 gaaaatacac ttaaagaaat aaaaaaacta ctaactaaaa tataaaataa tatcaatact
     4141 atctaaagca atgataaaaa actatgaatg gagttacaat tatgaataaa tcatatatgc
     4201 taaaatacac atataaatgc cctactaatt tatttcatgg agataaaaaa tatactctaa
     4261 catacactaa gagagttatg agtaatttag tatatgatat gggataatct agaaaataac
     4321 tcctttacat gtttagaagt acaatcattg tttgatgata tatttaaggt gaaattatga
     4381 tgaataatac aattggagaa aagattaaac aatatagaaa atcgataaat atgtcaaggg
     4441 atgagctagc caacaagatt ggttgttcag ttcatgctat agctaaatat gagcaaaatc
     4501 aaagaatgcc tagtttagat atgataagaa aaatagctac tgctttatca attgctataa
     4561 ataaactcat tgttgaagaa aatgatttag atactttaac aaataataat attaatttaa
     4621 aagtatttga taaagagttg gatttagtaa ttcacatatt agaaaattat ggttatgata
     4681 taaaaacaga ctataaaaaa aagtttgaaa ttacagataa tgcagataca ataaaaccaa
     4741 ttaacctaaa cttctctgac ttcaaagact tctccaaaaa tctaaattgg gtgataaatg
     4801 gtcttgttga aaattttata caagaaaact ctaatggtct tattgaagat gaggatgaag
     4861 aatattagta ttgcacattt tcaaaatatg agtttaccta ttaactttta tacattcata
     4921 gttaataaat aaaaattaga tatgaaagga gctaaaatca tgaataaatc atatatacca
     4981 aaatacacac ataaattccc tactaatcta cctcatggag ataaaaaata tactttaaca
     5041 tacattagaa gaatgataag tgaatttgta tatgatatgg gtaacctaga aaataacccc
     5101 tttacattcc cagaagtaca aacattactt gatggtataa ccgtaggcgg acataaatta
     5161 agtgaccaaa atcaaatact aaatataaaa tcatcatggg attatgtaat aaaattaagt
     5221 aacaaagaaa acttatcttt aaataaagat acaatttgct ctatacataa aatagttgca
     5281 aaagatgaag ctctaatcgt tggagatttc agaaatggta atataggtat agctggtact
     5341 actcaatatg aatgcataga agccactcta ttaaatgatt tatttgtaaa tgacataaat
     5401 atcataaata aaataactaa ccccttagaa aaggctataa taataaattt atggttatcc
     5461 tattgccaat tcttctatga tggcaacaaa agaactgcta gacttacctc aaacctaata
     5521 cttataagta atgatatagg agttctatca ataccagcta aacataaaca agaatacaac
     5581 acattaatgc taaactttta tgaaacacta gaagctgatg aagttataaa attcttatta
     5641 gagaaatgta ttacattctt cgatggatat aactataaat catataagga gttgtttaaa
     5701 aatggcaact aaaagagcta atggagaagg ctctatcgga aaatataaag atggttggcg
     5761 ttctcgtata atgattggtt ataatgaaga tggaaaacca ataagaaaag aattttatgg
     5821 taaaactcaa aaggaagtta aagataaact tgatgcttat aaaaaacaat attatctaag
     5881 ttctgatatt tcagatgata aaataacttt agaacattgg ttttacaatt ggttatttga
     5941 atatagaata aaagatttaa aaccaaagtc atttgaaaga tatgaaagca tctatcgtaa
     6001 ttatattaaa gatactgatt taggcaatgt taagttaaag gacttaagag tatctcacat
     6061 acaaaaatat tacaatagat tgcttgattc caataaaagt atacctacta taaggcaaat
     6121 aaacactaaa ctgaaaacct gcttatcaga agctgaaaaa caaggtctta tacaacgtaa
     6181 ttattgtact atggtaaata taccagttga gaaaaaggaa aataagcttg aaatattgac
     6241 tcttgaacaa caaaagagtt ttataaaagc tatagatggt cataaattag aggtattatt
     6301 tttacttgct cttggtactg gacttagatt aggagaatta ttaggactta aatggtttga
     6361 tattgatttt aaaaagtcta atttaacagt taaaagaact cttcaaagaa catattttat
     6421 agataaaact ggaaatagag agcttaaagt attagagcaa gagccaaaga catcaaattc
     6481 ttacagaact gtacctattc caaaagatgt tttaaataaa ttgaaagagc ataaagtaaa
     6541 tcaaaattcg gatatattaa aggctggaga attatataga aatgataatt atgtattttg
     6601 caatgaatta ggtatcccta tagatgataa aagaccttta aggaatctaa agtctatatt
     6661 aaacagttta gatatagagc ctataaagtt tcatggtctt agaaaaactt atgctactag
     6721 actttttgag aatgatgttc ctcctaaaac tgtacaagtt ttaatgggcc attatgatat
     6781 atcaataact ttaaatatat atactcaagt tatggaagat aagaaggttg aagctgtaga
     6841 aaagttggat aaaatatttt cattgtaaaa atcaaataca tataaatatt gtaatttcat
     6901 atagttaatt acaaaatcaa aataaggata caaattaatt ttatattatc ttgtatcctt
     6961 atttcgtatc tagttttaat tatttatctt ggatagcctt tatgatacat taatccattt
     7021 tctaacatta attcttgagc caagtatata tgacccttac agtgcatatc tgccacacct
     7081 tcacttataa gtttatatgt ttcagaagta tataaataat ccatactttc ttccaagctc
     7141 ttacctgttt gcttagaaaa ttctaatatg atttttttat atagtatttg taataatgtt
     7201 ttatttgctt ccataatcac tatacctcct cttcatacct aaactttaaa tatttatcta
     7261 ttacattttg attggttata catatttgtt catttggttt atagtatttt aatcttttta
     7321 aagcttcatc tttttcaata agattatcct gatacagttc tattgtattg taaatcttat
     7381 catcagcaat accacctatt ataatgtcat atttcatata tatatcactc atatttctac
     7441 attctattat aaaatcaagc cattctaaat tataactatt gaagattttt accttgcagt
     7501 tttcttttaa tttatcaata tctaaagaat aactatttaa atatgctttt ttacctcttt
     7561 ttttaaaacg ctctgtccag ttaattgctt gtgtctttat actggttaca tagaatcctt
     7621 ttccaaaatc taaagaatct cttgaaaagg taatactagg agtctttacc acacaatatg
     7681 aaccatgata tacaatcatt atatcacacc cttttcttta agtacttgca ttaaatcttc
     7741 cactatgtaa cctttacttt gagaatgtag tgcttcatag caaggtatta tatagttatc
     7801 taaaatatca gtatcatgtt taagaagttt ataaactcct tctgaatcta tattcaattt
     7861 ttctgctaaa gcttcaatgc aaaaaataga aaattctagt tttctttcat ccattacaaa
     7921 cctcaactcc tatcaagctt atataatata tatttttact aattaacaac ttaaactatc
     7981 ttacatttat aaaaatatta tatcataaag tagatattaa aattctatgt tacctattat
     8041 cataataaac aaataaaata ataaacaaat aaaaactttt tatacaacct aaatgcaccc
     8101 caatataccc ccaaaaaaac ctcttttcct gtggttgggg tacggttggt ggattttatc
     8161 tccaaaataa aaaatactag tcaacaaaga ctagtatttc caagcatttc atcattcttt
     8221 ttttaaaatt ggtggagacg aggggagtcg aacccctgtc cgcaaatctt ttccttgctg
     8281 catctacatg cttagccaac ttcactaata tctcacctca catagcgcca agtggcaggc
     8341 tctatgctta gccagtccca attaatctac ttcaagtcga gacaccccta aagtagttcc
     8401 ctgcataatg acgtccagtc ttagtcggca ggaggactaa gagggacgag cagcaactag
     8461 gctgctatcg cgaaattatc gtctgcgttt attttaaagt tccccagttt ttacgtgacc
     8521 cagagtaaca cgacatgcag cctcaagttc caaacccacg tcgaaacctt tacgccccct
     8581 tgagtagttt atagtccagt agacttttcg caaactttat caccattttt attaaatttt
     8641 taatgtatat ataatataat acttttaaga ataaaaatca atgttatgtt ctagataata
     8701 aagaatttta ttctaaaaaa tacagtttta tctagtttaa tacttacctg tcatttctct
     8761 ttctatacgt ctttgtgcat ctttcttagc catatcttga cgtttatcat atagtttttt
     8821 accttttgct aaggcaatct ctactttaac tttaccgttt tttagataca aactaagtgg
     8881 tataagtgaa taccccttta tagtagttgc tccaataagt tttcttattt catgcttatg
     8941 aagtaataat tttctaactc ttaaagaatc aacattgaat atatttcctt gctcatatgg
     9001 acttatatga acttgtttta aaaatacttc gctattatct acagatgcaa atccatcact
     9061 taggttaact ttgccttgtc ttattgattt tacttctgtt ccttttaatt caattccaca
     9121 ttcataagtt tcttctataa aatattcatg tcttgctctt ttgtttgttg ctaatatttt
     9181 tttaccagcc atataaatct caccttcttt gctagaaatt tcttccataa atgttagaaa
     9241 tttactaaat atttaatgtt gccaaattat ttgactaact tgtatgataa catatttagg
     9301 cattttagtc aatatttcac tgatatatta agttagttag gctatatata attaagtaat
     9361 ataaatgtat gaaaatagct aaaaaactac ttcgaatata cttaatacta aactaactct
     9421 aaccagtaat actaatttat aaatttcaaa acaaaaatat tacatattat a
//
LOCUS       DAELLV010000144         9324 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.144, whole genome shotgun sequence.
ACCESSION   DAELLV010000144 DAELLV010000000
VERSION     DAELLV010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9324
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.144"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(173..889)
                     /gene="dapD"
                     /locus_tag="KPD58_002577"
     CDS             complement(173..889)
                     /gene="dapD"
                     /locus_tag="KPD58_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HBG0266335.1"
                     /translation="MVNLNDAYEIARYIKEVKKKTPVKVYVNTNSLHFQSTDKFKVFG
                     SNGSYIFVGDYEDIMEVLDKHGNDITDTHVEYDRRNSAIPLKNTLSEHARIEPGAIIR
                     DMVTIEKNAVVMMGAVINIGAVIGEGSMVDMNAVIGARGTLGKNVHLGAGAVVAGVLE
                     PPSATPVIVEDDVLIGANAVILEGVRIGKGAVVAAGAVVTTDVEAGAVVAGSPAKVIK
                     MKDEKTADKTKLMEDLRNLD"
     gene            complement(1149..1898)
                     /locus_tag="KPD58_002578"
     CDS             complement(1149..1898)
                     /locus_tag="KPD58_002578"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBG0266336.1"
                     /translation="MLKVIATGYDGKMGKILADTIREDNELELVCVAARGLDSYEGDL
                     KIYEDMSTIVEEADVVIDFSHHSNLDNILSYVLKTKTPLVIATTGYNDDEMNKIREAA
                     KVIPVFQSYNMSLGVNVLVKLVKDAAKLLEGFDIEIIEKHHNRKVDAPSGTAVMIANA
                     IKEVLPNVENNYGRYGRSAKRQPNEVGIHAIRGGNIVGEHDALFAGDNEVLTISHQAQ
                     SRGIFASGSIAAAKYLVKQKPGFYNMDSMLG"
     gene            complement(1965..2852)
                     /locus_tag="KPD58_002579"
     CDS             complement(1965..2852)
                     /locus_tag="KPD58_002579"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBG0266337.1"
                     /translation="MIFKGSAVALVTPFTKDNKVDFDKLGELVEYQIANGTDAIVSCG
                     TTGEANTMTDEEQLATIKYVVEKVNKRVPVIAGSGSNDTMHSVNLSQEAEKLGVDALL
                     IITPYYNKANKAGLKRHFETIANSVKLPIILYNVPGRTCVNISPSLIVELAKIDNIVA
                     VKEASGDLGQVAEIASLVPDDFAIYSGNDDTILPLLSLGGQGVISVLANVCPQETHDL
                     VAKFFEGDIEGSRKLQLGMDALIAALFIEVNPIPVKTAMNLLGFNVGDLRLPLAEMDP
                     ANLEVLKKELVNFGLKVNA"
     gene            complement(2969..3970)
                     /locus_tag="KPD58_002580"
     CDS             complement(2969..3970)
                     /locus_tag="KPD58_002580"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBG0266338.1"
                     /translation="MKKVNVAIVGATGMVGRTFLKVLEERNFPIENLFLFSSSKSAGS
                     KVMFCGKEYIVEELKETSFTGRGIQIALFSAGGSISEKYAPIAASNGILVVDNSSQWR
                     MNPDVPLVVPEVNPEAIKNHKGIIANPNCSTIQAMVPLKALDDKYKIKRVVYSTYQAV
                     SGSGVKGVEDLEKGMNGNPTGFYPHQISYNCLPHIDSFTENGYTKEEMKMVNETMKIL
                     DNYDLKITATTVRVPVKNGHSESINVEFNNPFELDDLVKTLEEAPGVVVVDNPAKNEY
                     PTAVEFDDRDEVFVGRIRRDFSVDNGVNLWVVADNIRKGAATNAIQIAEMALSYDLV"
     gene            complement(4395..5276)
                     /locus_tag="KPD58_002581"
     CDS             complement(4395..5276)
                     /locus_tag="KPD58_002581"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBG0266339.1"
                     /translation="MLFKGSAVALVTPFTEDNNVNFEKLGELIEYHIENGTDALVVCG
                     TTGEATTMSESEIFAVIKYTVEKVNKRIPVIAGTGSNNTMLSVHMSQEAEKLGVDGLL
                     IITPYYNKTNEKGLKLHFETIANSVKLPIILYNVPGRTKVNIKPSVVAELAKIDNIVA
                     VKEASGDLAQVAEIAKLVPKDFAIYSGNDDTILPLLSLGGSGVISVLANICPKETHDL
                     VTKFFEGDIEGSKKLQLDMDALIAALFIEVNPVPVKTAMNILGFNVGDLRLPLAEMEE
                     TNLNVLKQELTNFGFKF"
     gene            5599..6792
                     /locus_tag="KPD58_002582"
     CDS             5599..6792
                     /locus_tag="KPD58_002582"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBG0266340.1"
                     /translation="MDTLKELFKIGSGPSSSHTMGPQRAAERFKNENPDAESFRAILY
                     GSLAATGKGHLTDYIIEKTIAPKKVEIVWEEDIIKDFHPNGMKFEALDKDKNVTAEWT
                     VYSVGGGTIAEEGQRNSKSNSIYHLDTMDEIVKWCKENNKTLVDFVLECEPKDIKDYI
                     KTIKDAMRKSIDDGLSTDEIIPGKLLLKRRASTFYNAYKKDKSFSTLVYAYALAASEQ
                     NASGNIIVTAPTCGSAGVIPGIFFAMQDFYNYDDEKIIEALLVAGIIGNIIKTNASIS
                     GAEVGCQGEVGAACSMAAAAVAYLKGGTIDHIEYAAEIALEHHLGMTCDPVYGYVQIP
                     CIERNAMAAQRAYDAANYALLTDGSHSVSLDQVVETMKETGIDMMDKYKETAKGGLAK
                     HFFSC"
     gene            6998..8116
                     /locus_tag="KPD58_002583"
     CDS             6998..8116
                     /locus_tag="KPD58_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HBG0266341.1"
                     /translation="MREASYLQEQLYKHRKSLNPIAEIGRKEYKTSKYIRDYLDKLGI
                     EYEVYLETGIVGVIEGKRPKKDIAFRADIDGLHSEEGVKHLCGHDGHATILLGLIEYL
                     NDNKEDLNDNIVFIFQPAEEGPGGAKALIDEGVLRKYNIDEIYGLHIYPELPEGYVGT
                     REGYFMAQIGDIDIEIVSKSGHGAMPQNGIDGIVIASNFVSSLQTIVSRNISPIDNAV
                     LTIGRIEGGARRNIIAENIKMEGTMRCFSPEVYEKMKLRVRELARGFELAYNCKVNIN
                     IIDDYIAVKNDKDLYQEFVEAIGSDIVVELEPLMISEDFSYYQKEVPGLFFMLGSRNE
                     EKGFVNGLHNINFNFDEKICVNALNVYSKLLKYKEAID"
     gene            8247..8492
                     /locus_tag="KPD58_002584"
     CDS             8247..8492
                     /locus_tag="KPD58_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HBG0266342.1"
                     /translation="MKEKVQREKKITNGKNTKILTNDDIMKYEIASELGLMDKVGERG
                     WAGLTAKEAGRIGGMLTSRKKRLKKIVEEQNKKDESI"
     gene            8479..9225
                     /locus_tag="KPD58_002585"
     CDS             8479..9225
                     /locus_tag="KPD58_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG0266343.1"
                     /translation="MSQYSDFAFVYDELMNEVDYNGWVKYIEDIIDSEGVKVKNILEL
                     ACGTGNLTIPLTKKNYDIAGIDISDEMLSVAREKAEKEGVELVLLQQDISELDFEISD
                     LDCVLCACDGFNYITYDDDLENIFSKTYELLKKDGIFIFDISSFYKLANILGNNMYGE
                     NREDIAYMWQNYFDEEENLIEMELTFFIKDECGKFERFEEIHQQRAYTEDEIVDMLEK
                     SGFKDIKYYGDFTFDKPSEESQRIFFVCKK"
BASE COUNT         3350 a         1358 c         1425 g         3191 t
ORIGIN      
        1 atataagaat gaataagaga attgtaaaaa aataaaatga ttataaataa aagataaaaa
       61 tattgtaaaa aagagggtgc ctctggtgaa ttaaattcac acagagacac ctttttaatt
      121 aattttttaa attagtatat tattgtttaa ttaatagcat agtactattt aattagtcta
      181 aatttctaag gtcttccata agcttagttt tgtcagccgt tttttcatct ttcattttta
      241 taactttagc aggagaacca gcaacaacgg caccagcttc tacatcagta gttacaacag
      301 caccagctgc aacaacagca cctttaccga ttctaacacc ttcaagaata actgcattag
      361 ctcctattaa aacatcatct tcaactataa caggagtagc agaaggtggc tctaaaacac
      421 cagcaacaac agcaccagca cctaagtgaa catttttacc aagagttcct ctagctccaa
      481 taacagcatt catatctacc atagaacctt caccaataac agctcctata tttataacag
      541 ctcccatcat tacaacagca tttttttcta tagtaaccat gtctcttatt attgcacctg
      601 gctcaattct tgcatgttca cttaaagtat tttttagagg aatagcagaa tttcttctat
      661 catattctac atgagtatct gttatatcat ttccatgttt atctaatact tccattatat
      721 cctcatagtc acctacaaat atataagaac cattacttcc aaatacctta aatttatcag
      781 ttgattggaa gtgtaaagag ttagtattta catatacctt aacaggggtt ttctttttaa
      841 cttcttttat ataccttgct atttcataag catcgtttaa atttaccatt tgttaattct
      901 ccttgtattt cattatattt cttttattat atacttattt gtcaatttta gtatatactt
      961 acttcttatt atttatattt tatttagttt ttattgtata gacatagtat taaataaatt
     1021 tattatatat gtttgatttg tattgcgtat tattatatct atattgcata tgtttgattt
     1081 gtattgcgta ttattatatc tatattgcat atgtttaatt tatattgtgt attattatat
     1141 ttatactatt atcccaacat gctgtccata ttataaaatc ctggtttttg cttaactaag
     1201 tatttggcag cagctataga accacttgca aagataccac gagattgagc ttgatggctt
     1261 attgtaagaa cctcattgtc tccagcaaat agagcatcat gttcgccaac aatatttcca
     1321 cctcttatag catgaatacc aacttcattt ggttgtcttt ttgcactacg accatatcta
     1381 ccataattat tttcaacgtt tggcaaaacc tcttttatag cgttagcaat cataacagct
     1441 gtgccacttg gggcatctac ttttctgttg tgatgttttt ctattatttc tatatcaaat
     1501 ccttctagta atttagctgc atctttaacc aattttacta atacatttac accaagagac
     1561 atattatatg attggaatac aggaataact ttagccgctt cacgtatttt gttcatttca
     1621 tcatcattat atccagtagt agcaattact aaaggtgttt tagtctttaa aacgtaagat
     1681 aagatattat ctaaattaga gtgatgagaa aaatctataa ctacatcagc ttcctcaact
     1741 atagtactca tatcttcata aattttaagg tcgccttcat agctatccag acctctagct
     1801 gcaacacaaa ccaattctaa ttcattatct tctcttatag tatcggctaa aatcttaccc
     1861 atttttccat cataaccagt tgctataact tttaacataa tttcctccta tgattaaggg
     1921 ctaacttttg accaaactta ctttttacca tgcattaatc tttactatgc attaactttt
     1981 agtccaaagt taactaattc tttctttaat acttctaaat ttgctgggtc catttctgct
     2041 agaggtaatc ttaaatctcc aacattgaat cccaataaat tcattgcagt cttaactggt
     2101 ataggattta cttctataaa taaagctgcg attagagcat ccataccaag ttgtaatttt
     2161 ctagaacctt caatatcgcc ttcaaagaat ttagctacca aatcgtgagt ttcttgaggg
     2221 caaacattag caagtactga tataactcct tgacctccta aagataataa aggaagtata
     2281 gtatcatcat taccagagta gatagcaaag tcatctggaa ctaaactagc tatttctgca
     2341 acctgtccta ggtctccact agcttctttt acagcaacta tgttatcaat tttagcaagt
     2401 tcaactataa ggctaggact tatatttaca caagttcttc cagggacatt gtaaagtata
     2461 attggaagtt taacactgtt agctattgtt tcaaaatgac gcttaagtcc agctttatta
     2521 gctttattgt aataaggtgt tattattaat agagcatcta ctcctaattt ttcagcttct
     2581 tgacttaagt taactgagtg catagtatca ttagaaccac ttccagctat aacaggaact
     2641 cttttattaa ctttttcaac aacatactta atagtagcaa gttgttcttc atcagtcata
     2701 gtattagctt caccagtagt accacaagat actatagcat cagtaccatt agctatttgg
     2761 tattcaacta attcacctaa tttgtcaaaa tcaactttgt tatcctttgt aaatggtgta
     2821 actagagcaa cagcagaacc tttaaatatc atataaaatc aatcccctct tttttatttt
     2881 tgtgttttat attacagtta cttctaatgc ttgtttttga gttactatta atattttaca
     2941 aaattatata tttatacaag tttgtatatt atactaaatc gtatgataaa gccatttcag
     3001 ctatttgaat tgcgttagtt gcagcacctt ttcttatatt atcagcaaca acccaaagat
     3061 ttacaccatt atcaacacta aaatctcttc ttattcttcc aacaaatact tcatctctat
     3121 catcaaattc tacagcagtt ggatattcgt ttttagcagg attatcaact actacaacac
     3181 ctggagcttc ttcaagtgtt tttactaaat catctaactc aaatggattg ttaaactcaa
     3241 catttataga ttcactatga ccatttttta ctggaactct tacagtagtt gcagttattt
     3301 ttaaatcgta gttatcaagt attttcatag tttcatttac cattttcatt tcttctttag
     3361 tgtatccatt ttcagtgaat gaatctatat gaggaagaca gttgtaagag atttgatgag
     3421 ggtagaatcc agtaggatta ccgttcattc ctttctctaa atcttcaaca cctttaacac
     3481 cagaaccaga tacagcttgg taagtagagt aaacaactct cttaattttg tatttatcat
     3541 caagagcttt aaggggaacc atagcttgta tagtagagca gttaggatta gctattatac
     3601 ccttatgatt ttttatggct tctggattaa cctcaggaac tactaaaggc acatctggat
     3661 tcattctcca ttgactagag ttatctacta ctaagattcc atttgaagca gctattggag
     3721 catacttttc actaatactt ccaccagcag agaataatgc tatttgtatg cctctaccag
     3781 taaatgaagt ttcctttaat tcttctacta tatattcttt accacaaaac attactttag
     3841 aaccagctga tttagaagaa gagaataaaa atagattttc tattggaaaa tttctttctt
     3901 ctaatacttt taaaaatgtt ctacctacca ttccagtagc accaactatt gctacattta
     3961 cttttttcat ataaattcac cccaatttta aattattaaa acattatttt attttagtta
     4021 tacaagtaag atatgttaat atttttatat atccaaaaac tttaatatta tattaacaac
     4081 tttaattata tacaaaaaac gtacaaaggt atactttgta cgtctaattt atcactcaaa
     4141 gtaaaccctg tagctcatca cctacaaaaa taagtgacag tattacacat gttatgtgca
     4201 atcccagaaa atattattgc aagaaatatt ttcttcggct gtaaatcctt tttccaatca
     4261 ttaacacctg ccagctacta ttggatactc ttagttacag ctacctctac cctaggttaa
     4321 taggatatta aaatttgatt attctaataa taatacatat ttaagaaaaa atcaacttat
     4381 ttatataaat attgctaaaa tttaaatcca aaatttgtca attcttgttt taacacatta
     4441 agattagttt cttccatttc agctagagga agtcttaaat caccaacatt aaaacctaaa
     4501 atgttcatgg cagttttaac aggaactgga tttacttcta taaataaagc agcaattaaa
     4561 gcatccatat caagttgtaa tttttttgag ccttcaatat caccttcaaa gaatttagtt
     4621 actaaatcat gtgtttcttt aggacatatg ttagcaagta cagagataac accacttcca
     4681 cctaatgata atagtggtag tatagtatca tcattaccgg agtatatagc aaagtcttta
     4741 ggtactaatt tagctatttc tgcaacttga gctaagtctc cacttgcttc cttcacagca
     4801 actatattat ctattttagc aagttcagcc acaacagatg gctttatatt cactttagtt
     4861 cttcctggta cgttgtataa aataattggc aacttaacgc tatttgctat tgtttcaaag
     4921 tgaagtttaa gacctttctc attagtctta ttatagtaag gagttattat taaaagacca
     4981 tccaccccta atttttcagc ttcttgactc atatgaacag acaacatagt attgtttgaa
     5041 ccagtacctg ctataacagg tattctctta ttaacttttt caactgtata ttttattaca
     5101 gcaaaaattt cagattcact catagtagtc gcctcacctg ttgttccaca aacaactagt
     5161 gcatctgtgc cattctcaat gtgatattcg attaattcac ctaatttttc aaagttgaca
     5221 ttattatcct ctgtaaatgg ggtaactaaa gcaacagcag aacctttaaa taacatataa
     5281 tcaaatctcc ttttttatct aaatttaatt ttttaagtac aataataatt atagtacaaa
     5341 ttaattaatt ttaataggta tagttaatat cttaactgtt taaataagat atttatatta
     5401 tattaatatt agaaaaattt taaataaatg ttttattctc aaaatatgca tattaattta
     5461 ataaaatata ccaaaatgga aataaaaaat aaattttatg agaatttaat aactaataat
     5521 taatatattt aggttataat aaatatgtgc gtaaaatcta acaataagca ataataccgt
     5581 aaatttagga ggaataatat ggatacttta aaggaacttt ttaaaatagg aagtggacct
     5641 tcaagttctc atactatggg accacaaaga gcagcagaga gatttaagaa tgaaaaccca
     5701 gatgcagaaa gttttagagc aatattatat ggtagtttgg cagccacagg gaaaggtcat
     5761 ttaactgact atataattga aaaaactata gcacctaaaa aagtcgaaat tgtttgggaa
     5821 gaagatatta taaaagattt tcacccaaat gggatgaagt ttgaagcttt agataaagat
     5881 aaaaatgtaa cagctgaatg gactgtatat tctgtaggtg gagggactat agcagaagaa
     5941 ggccaaagaa acagtaaaag caatagtata tatcacttag atactatgga tgagatagta
     6001 aaatggtgca aagaaaataa taagactttg gtagattttg tgttggaatg tgaaccaaaa
     6061 gacataaaag attatataaa aactataaaa gatgctatga gaaaatctat tgatgatgga
     6121 ctaagtacag atgaaataat accaggcaag ttattattaa aaagaagagc aagtacattt
     6181 tataatgcat ataaaaaaga taagagcttc tcaacacttg tatatgcata tgcattagca
     6241 gcttcagagc aaaacgcttc tggtaatata atagttacag caccaacttg tggttctgct
     6301 ggagtaatac caggaatatt ctttgcaatg caggatttct ataattatga tgacgaaaaa
     6361 ataattgaag cactacttgt agctggaata ataggtaata taataaaaac taacgcttca
     6421 atatctggag cagaggtagg gtgtcaaggt gaagttggag ccgcttgttc tatggcagca
     6481 gcagcagtag catacttaaa aggtggaact atagaccata ttgaatatgc ggcagaaata
     6541 gcacttgaac atcatttagg aatgacttgt gaccctgtat atggatatgt tcaaattcca
     6601 tgtatagaaa gaaatgcaat ggcagctcaa agagcttatg atgcagctaa ttatgcatta
     6661 ttaactgatg gctcacattc tgtctcctta gaccaagttg ttgaaacaat gaaagaaact
     6721 ggtatagata tgatggataa gtacaaagaa acagctaagg gtggacttgc taaacatttc
     6781 ttctcttgct aaaaataaaa ttttccaata ataattcctc tgttttgatt tatgaaacag
     6841 gggaattatt attaatggtg aattatttaa taatgcaaaa tatttttata ttcaatttag
     6901 ctttttattt ttaattagta taacatgcta tataatattt taattataaa gaaatataga
     6961 aatatttttt aataaattag aaattggagg agattacatg agagaggcat catatttaca
     7021 agaacaactt tataaacata ggaagtcatt aaatcctatt gcagagattg ggagaaaaga
     7081 gtataagact tcaaaatata taagagatta tcttgataaa ttaggaattg agtatgaagt
     7141 ttatttagaa acaggtatag taggtgtaat agaaggtaaa agacctaaaa aagatattgc
     7201 ttttagagct gacatagatg gtcttcatag tgaagaaggg gtaaaacatt tatgtggtca
     7261 tgatggacat gctactatat tacttggatt aattgagtat ttaaatgata ataaagaaga
     7321 cttaaatgat aatatagtat ttatattcca accagcagaa gaaggtcctg ggggagcaaa
     7381 agctttaata gatgaaggag tgcttagaaa atacaatatt gatgaaatat atgggcttca
     7441 tatatatcca gagttaccag aaggttatgt aggtacaaga gagggctatt ttatggctca
     7501 aataggagat attgacatag aaatagtgtc taaaagtgga catggggcta tgccacaaaa
     7561 tggtattgat ggtatagtga tagcttcaaa ctttgtaagc tcacttcaaa ctatagtgtc
     7621 tagaaatata agcccaatag acaatgcagt gttgactatt ggtagaattg aagggggagc
     7681 tagaagaaat ataatagctg aaaatataaa gatggaaggt actatgagat gtttcagccc
     7741 agaagtgtat gagaaaatga agcttagagt tagagaactt gctagagggt ttgaattggc
     7801 ttataactgt aaggttaata taaatattat agatgattat attgctgtta aaaatgacaa
     7861 ggatttgtat caagagtttg tagaggctat tggaagtgat attgttgtag agttagagcc
     7921 acttatgata tcagaagatt tttcttatta tcaaaaagaa gttccaggat tattttttat
     7981 gttgggttca agaaacgaag aaaaaggatt tgtaaatggt cttcataata taaactttaa
     8041 ttttgatgaa aagatatgtg taaatgcatt gaatgtttat tcaaaattat taaaatacaa
     8101 agaagcaata gattaggaag ataagttata aagattaaaa agcataaata cctaaattta
     8161 aaaaaagtgt attaataaat accccttaca tatttgtata aatttatttt aattgacaaa
     8221 aatacaaata agaaggggtg atttttttga aagaaaaagt acaaagagaa aagaagataa
     8281 ctaatggcaa gaatactaaa atattgacaa atgatgatat aatgaaatat gagatagcct
     8341 cagaactagg tcttatggat aaagttggtg aacgtggatg ggcaggactt acagctaagg
     8401 aagcaggaag aattggaggt atgcttacat cacgcaaaaa gaggcttaaa aagattgtgg
     8461 aggagcagaa taaaaaggat gagtcaatat agtgattttg cgtttgtata tgatgaactt
     8521 atgaatgaag ttgattataa tggttgggtt aaatatatag aagatataat agatagtgaa
     8581 ggtgtaaaag taaaaaatat attggaattg gcatgtggca cgggtaatct tacaattcca
     8641 ttgactaaaa aaaattacga tatagctggt atagatatat ctgatgagat gcttagtgta
     8701 gcaagagaaa aggcagaaaa agaaggagta gaattagttt tacttcaaca agatatatca
     8761 gagcttgatt ttgaaatatc tgatttggac tgtgtacttt gtgcttgtga tggatttaat
     8821 tatataactt atgatgatga tttggaaaat atattttcaa agacatatga attattaaag
     8881 aaagatggaa tattcatatt tgatataagt tctttttata aattagccaa catattggga
     8941 aataacatgt atggagaaaa tagagaagat atagcgtata tgtggcaaaa ttactttgat
     9001 gaagaagaga atctaattga aatggaactt acattcttta ttaaagatga atgtggaaag
     9061 tttgaaagat ttgaagagat acaccaacaa agagcatata ctgaagatga gatagttgat
     9121 atgcttgaaa aatctggatt taaggatata aaatattatg gggattttac atttgacaaa
     9181 cctagtgaag aaagtcaaag aatcttcttt gtatgtaaaa aataagatat tcttgactat
     9241 ataagataat tattaaaaat aaccatgata taaaaatata gaaaatgcag ttttatagat
     9301 taaaagtaaa gtgatgatac ttta
//
LOCUS       DAELLV010000145         9203 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.145, whole genome shotgun sequence.
ACCESSION   DAELLV010000145 DAELLV010000000
VERSION     DAELLV010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9203
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.145"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(757..1395)
                     /locus_tag="KPD58_002586"
     CDS             complement(757..1395)
                     /locus_tag="KPD58_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBG0266344.1"
                     /translation="MLIHLVKKDILIVKKYVLLILFIAIGIPLFILWRLPPFAQILGF
                     VLSIIFSEFMLCQYLLLKESQYSKASVLLCSTPYPRRGLVQSKYVLFAMVFIYCTLVY
                     WIENLLIPQIGTFDLTYILIVLLISSIIYGVYMPIQYKLGYETTKFFFVVIIMASSFV
                     LPLTIAHSNFEIPFIYQYPSMIVNIVLFLLSLIILLASMFISIKIFSNKELV"
     gene            complement(1398..2255)
                     /locus_tag="KPD58_002587"
     CDS             complement(1398..2255)
                     /locus_tag="KPD58_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0266345.1"
                     /translation="MENILEVYNLNKTYTDFSLSNISFALPEDCITGFIGVNGAGKTT
                     TIRSILDVVKRDSGKIKIFGKDFDGNEKELKNRIGVVFDDSYFYEELSMAEMKSIIAP
                     SYDNWCEEDYQQNMKRFNLNPKQKIDTLSKGMKMKFALALALSHKAEFLIMDEPTSGL
                     DPLIRSQILDILTEYMKKCGRGVFFSTHITSDLDKIADMIVMIDNGRIILEREKDTLL
                     DEHRKVKGDIQFLNDETKKLFLNLEITGYGFIGITSKINEVQKNMPDILIERPTIEDI
                     MLAYIERRE"
     gene            complement(2257..2631)
                     /locus_tag="KPD58_002588"
     CDS             complement(2257..2631)
                     /locus_tag="KPD58_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0266346.1"
                     /translation="MKVLISNTSDKPLYQQIKEQIKEAIFTEELKEGDAMPSIRNFAN
                     DLKVSILTIRRVYEELENEGFLTRQVGIGTFISTGNLEILRDSKRRIVEQKMADMIKD
                     AKALGITQIELQEMMDILYQED"
     gene            complement(3780..4970)
                     /locus_tag="KPD58_002589"
     CDS             complement(3780..4970)
                     /locus_tag="KPD58_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBG0266347.1"
                     /translation="MSDMLLWFSVVLVLFAIGDLIASKTKAKVSAVFVTLLLFLILFV
                     TKAIPADIIEKAGMTAAASWSVPMIMFSMGTMLNVKQFIDEWRTVLTAWLGIVAVIVC
                     VSLCIPLFGKSTVLTSIPVINGALPATTIMTQAALEKGLTLAAATATVVFAIQKFIGT
                     PIASRAALQEANRLLVEYHEAKSKGIDLANVDTDKKEAENTGTKVKQAFCEKYDKYYS
                     TNVCIFFIALFSYLGYELSEIIHVNYSIVCLVVGVIVTRIGIVPKDILEKGKIKGFIN
                     MVVFAAVIPSLAKVSLTDLISLFVPIVGMFAASIIGIFLMMKVLPGWKIIGSKPLAFG
                     VGFCQMLGFPTTYLISNEVCNAVGETEEERAYLMSKIMPKLVVGGMACMISIVVAGLM
                     VPML"
     gene            complement(4986..6941)
                     /locus_tag="KPD58_002590"
     CDS             complement(4986..6941)
                     /locus_tag="KPD58_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HBG0266348.1"
                     /translation="MEKIKIESSDIFEYIFPHDISCSPDGKHIAYIISNINEEKDCYE
                     HDLYVMDIKTEKQIHMTQTKDVTSFSWISNTELLFTSKRNKPKSGTTDFYTISIEGGE
                     AKKAFSIPKACSVPVSLGNKLWLLTTKNPTDSKKSEPDRAVEGVDYWTFTDKPFIRDG
                     ENFSQRRRVTLELYQEGENITKAITPKFCEVAGVDVSSDKNRILYTGQIYEDCATPFS
                     GLWEYHIDSGETKELVPQGKYQISLAKYIGKDKVMLQASTLDRSITQNHDIFILDLST
                     NEINMIASPDGMYATLLDVDAVYGGGRSNKVIGDKFIGARICRTMTEFNEFDTKTGNI
                     RIITKVDAFTSFDIYDNTMYTVMLKDYELAEIYSIDMTTGTMKKMTAFSKPYLDTHKV
                     SLPEKLTFVAKNKEEVDGFVIPPIDAKEGEKYPAVLFIHGGPKWAYGYMFTHLKQCVT
                     SKGMYVIYCNPHGGDGYGEKFLEMVERWGYVDYEHLMEFVDTCIEKYPGIDADRLGVA
                     GGSYGGYMTNWIIGHTDRFKAAASQRGISNLITASLIIDFGDRIMKQTCGDKTPWNHE
                     EVLWNHSPIKYVKNVKTPTLFLHSDRDYRCFMGDTFQMFTALKQLGVDTEMYLFHGDT
                     HGLSRNGRPSNRIARANAIVDWFERYL"
     gene            complement(6958..9024)
                     /locus_tag="KPD58_002591"
     CDS             complement(6958..9024)
                     /locus_tag="KPD58_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HBG0266349.1"
                     /translation="MKEVVGSLKKITKERTENTMKKLFTSETFLEYQFISDCQISPDG
                     GYTAFIVKKADIKENGYTSQVYILNNKTGELKQITSINSVGAYAWEDENTILFPALRN
                     EKVKEAVKNGKQCMSYYALSLNGGEAEELFRLPIKGGKLNPIGKGLYAVIDSYDNDRP
                     IVEGLPEEEQQKKINAYNKRHYEHFKEIPYAVNGEGYISRKRKRLYIYDSNINELKAI
                     TAPMFNVVGMKISDGKILYIGQEFKDVKGLKNGVYVFDTNTNTNVCILEKDKYIIKGF
                     ELYQNQVILNLTDALSYGNGENGDFYTIDIDTKEMKLLSGHQHHCIGNTVTSDVKMGA
                     GQTTKVDGEYIYYTSTVDMDCIIERIHIPTGKQEKVTQTGSVDFIDVKDGNIVCVGCL
                     GNGLPEVYTVENGTLCKKTHLNDHILEEYKISVPEYIESKGSSKWEIQGYVYKPVDYE
                     VGKKYPAVLAIHGGPRLTYGPYFMHEIQVFTSAGYFVFFCNPRGSEGRGNAFADIRKQ
                     FGDIDYIDFMEFTDTVLEKYTDIDKTKLAVEGGSYGGFMTNWIIGHTNRFAVACAQRS
                     IANWSGMEGTTDIGYYFCKGQTGASHMENHELQWKQSPLAYADKCVTPTLFLHGEKDY
                     RCYMQEAFQMFSALKIHGCPTKLCLFEGENHELSRSGRPKQKLQRLVEMLDWFSVYIK
                     KDNIGQ"
BASE COUNT         3140 a         1654 c         1094 g         3315 t
ORIGIN      
        1 cgtataaaat taaaagtaaa gtaatataga aaatatgaat atagaaaaac tttaataaga
       61 aaataaatta tgaataaata aaagaaacgc aatactgtat atagatgatt atttagaaaa
      121 ataaaatctc ttttaataaa aataagcttc aaaataaact tttagttcat aaagcttagg
      181 agtaactaaa ttgagtgcaa gtgtatctac tgttcaaaat aaatagttca taaagcactc
      241 ttttttatat taaatcaatt atattttcgt catttcagca atgaaaaaga agcttatctc
      301 tattttgaga cagcttcttt ttattaacgt cctacttttt attagttttc taaaattaaa
      361 aagctttatt tataggattc cttagattta tattttgacg atataatttt ttaaaaacac
      421 cttaaaagat ggtatacaat acacacaatc atactcataa ttgaaaaata atcaatggaa
      481 aacaaaatta ctggatattc cctattaaaa aatacaaatt gattcacaag aaacatataa
      541 tctataatat tatgatgtta ctctcccaca ccaagtaact ttattttcaa gtaacctttt
      601 cataccatct aacttcactc aataaaaatg gcaatataaa tatatactct attttttact
      661 tttaaatatc tgacaagctt tatatactat tggaagtaag atacaaagta aaacagccca
      721 atctggtaaa tgtggcatat aaaagtcccc cttaccttat acaagctcct tattactaaa
      781 aattttaata gaaataaaca ttgaagctaa cagtataatt aaacttaaaa ggaacaatac
      841 aatattcact atcatggatg gatactgata tataaatgga atttcaaaat tgctgtgagc
      901 tatagttaat ggtaatacaa atgatgaagc catgattatc accacgaaaa agaattttgt
      961 tgtttcatat cctaatttat attgaatagg catataaact ccataaataa tagaacttat
     1021 taataaaaca atcaaaatat atgttaagtc aaaagttcct atttgtggaa taagtaaatt
     1081 ttcaatccag taaactaatg tacaatagat aaataccatt gcaaataaaa catacttaga
     1141 ttgaactaag ccacgtctag gataaggagt tgaacaaagt aatacagatg cttttgaata
     1201 ttgactctcc tttaataata gatactggca cagcataaat tctgaaaata taatggatag
     1261 tacaaatccc aatatttgtg caaatggagg taaacgccac aatataaata atgggattcc
     1321 tattgcaata aataatatca acaatacata tttttttaca atcagaatat cttttttaac
     1381 taaatgaatc aacataatta ctctctcctt tcaatatatg caagcataat atcttcaatt
     1441 gtaggacgct ctattaaaat atctggcata tttttttgca cttcattaat ttttgaggta
     1501 atccctataa aaccatagcc ggtaatttct aaatttaaaa ataatttttt ggtttcatca
     1561 tttagaaatt gaatatctcc ttttactttt ctatgttcgt ccaatagagt atctttttca
     1621 cgctctaata taatacgtcc attgtcaatc ataacaatca tatctgctat tttgtctaaa
     1681 tcagatgtta tatgagtgga aaagaaaaca ccacgaccac atttcttcat atattctgtt
     1741 agtatgtcaa gtatctgact acggattagt gggtctaagc cacttgtagg ctcatccata
     1801 attaaaaact cagctttatg cgataatgct aatgctaaag caaatttcat tttcattcct
     1861 tttgaaagtg tatcaatctt ttgttttggg ttaagattaa aacgcttcat attttgttga
     1921 taatcttctt cacaccaatt atcatatgaa ggtgcaataa tacttttcat ttctgccatt
     1981 gacaactcct catagaaata tgaatcatca aatacaactc caattcgatt ttttaattcc
     2041 ttctcatttc catcgaaatc tttaccaaag atttttattt ttccactatc acgctttacg
     2101 acatccaata tcgaacggat tgttgttgtt tttcctgctc cattaacacc tataaatcca
     2161 gttatacaat cttctggtaa tgcaaagctt atattagata atgaaaaatc ggtataagtc
     2221 ttatttaagt tataaacttc taaaatattt tccatattaa tcctcctgat atagaatatc
     2281 catcatttct tgtaactcta tctgtgtaat tcctagtgct tttgcatcct taatcatatc
     2341 tgccattttt tgttcaacta tacgtctctt tgagtctctt agtatttcta aatttccagt
     2401 tgaaataaat gttcctattc caacttggcg tgtaagaaag ccttcatttt caagttcttc
     2461 atacactcgt ctaatagtca aaatactaac ttttaaatca ttagcaaaat ttcttatgga
     2521 tggcattgca tccccttctt tcagttcttc agtaaatatt gcttctttaa tttgctcttt
     2581 aatttgttgg tataatggct tatctgatgt gttagaaatg agcactttca ttttttatca
     2641 cccccttttt atcatcttca gtgtgatgtt gttatataca cactatacac ttagagcact
     2701 tttttgtcaa tataaaagat gtaaaatatt actatgacaa aataaatatg aatgaaatat
     2761 tagagattgt taatagccat aaacactttt taaaaccaca taagatagaa tgtattaaat
     2821 atttattaaa agtaagaatt gaatatctaa aagaaaaagt aatcctgtat gaatgatttg
     2881 tttgatgata aattaagtta taaactattt ataaaaaaca taaaggaata aatccatttc
     2941 taattacaaa catactaaaa aatttagata gtaataaata gatagtaata aatgggatga
     3001 atttataaga atttggatag aggaaagagt tattcaagag tgtagactag aggtaaactc
     3061 tatataaaaa aaatttaaat tgggatatta ttatgtatgg aatatttgca gaaataacct
     3121 tgctataagt atagaagatt tttttgatta caaaagataa tactctaaaa tgtgaggatt
     3181 tcaacattca atactgtgct ttaaataata tcactccaaa ataccatcca ccttttacta
     3241 taattgaata ccctaattat tttaaaaagg aaaacattag aaacggaaaa ccttgtatat
     3301 ataaaataaa aaatactaac ttctaaaaca cggatactct ttgataaata aaacaaaaaa
     3361 taattttaat aattaaaatt ggctatctca aactagagat aagtctcttt ctcactttta
     3421 aaacgttgaa aatatattcg atttcgtaca aaaaagagtg ccttagaact aaaaagttca
     3481 ctttgtggca ctcccttttt tctcctcttt ttaatttttt ataaaaattc ttcaaaaata
     3541 ttattaaata ccctatatta tacataaatc aaattaaagt tgcattttag caaataaaaa
     3601 caaattataa ctattagcat agcaaataaa aaatctgctc tcttataaaa tcaaaattat
     3661 aattataaaa agaaaatatt cttataacaa attaactaat ataaaattat tttataaatt
     3721 aatactactt tttagcttct cctataaatt aacaccactt ttttaactat gaaacctatc
     3781 tacaacattg gtaccataag accagctacc acaatagaaa tcatacaagc cataccacca
     3841 actaccagct taggcataat cttagacatc aaatatgctc tttcttcttc cgtttctcca
     3901 acagcattac acacttcatt tgaaattaaa tatgtagttg ggaatcccaa catctgacaa
     3961 aaccctactc caaaagcaag aggtttagaa ccgataattt tccaacctgg taacaccttc
     4021 atcataagga atataccaat gatagaagct gcaaacatac ccacaattgg tacaaaaaga
     4081 ctaattaaat ctgtcaaaga aacttttgct aaagaaggaa taacagctgc aaacacaacc
     4141 atgttgataa atcccttgat ttttcctttt tccaaaatgt cttttggtac aataccaata
     4201 cgtgttacaa taacaccaac aacaagacat acaatagaat aatttacatg aataatctct
     4261 gaaagttcat accctagata agagaatagt gctataaaaa atatacaaac atttgtagaa
     4321 taatatttat cgtatttttc acagaaagct tgttttactt ttgtgcctgt attctctgct
     4381 tctttcttat cagtatcaac atttgctaaa tcaatgcctt ttgattttgc ttcatgatat
     4441 tctactaaaa gacggtttgc ctcttgtaac gctgctcttg aagctattgg tgtaccaata
     4501 aacttctgaa ttgcaaatac aactgttgct gtagctgctg caagtgtcag tcctttttct
     4561 agtgctgctt gtgtcataat agtagtagca ggtaaagctc catttataac tggtatgctt
     4621 gtcaatactg ttgattttcc aaacaaagga atacataatg agacacagac tataactgct
     4681 acaattccaa gccatgctgt caatactgtt ctccattcgt ctataaattg tttaacattc
     4741 aacattgttc ccatactaaa cataatcatt ggaacactcc aactagctgc tgcggtcata
     4801 cctgcttttt caataatatc agctggtatt gcttttgtga caaatagaat caaaaataaa
     4861 agtagtgtaa caaatacagc tgatactttt gcttttgttt tgcttgcaat taaatcccca
     4921 atagcaaata aaactaatac gacactaaac cataataaca tatcactcat agaataaccc
     4981 ccttattata aatatctttc aaaccaatct acaatagcat ttgctcttgc aattctatta
     5041 gatggtcttc catttctaga caatccatgt gtatctccgt gaaacaaata catttctgta
     5101 tctacaccaa gctgttttaa tgctgtaaac atttggaaag tatcccccat aaaacatcta
     5161 taatccctat cgctatgtaa gaacaatgtt ggtgtcttaa catttttcac atatttaata
     5221 ggtgaatgat tccaaagaac ctcctcatga ttccaaggcg tcttatctcc acaagtctgc
     5281 ttcataattc tatctccaaa gtcaataatt aaagaagctg taatcaagtt actaatacct
     5341 ctttgagaag ctgctgcttt gaaacggtct gtatgaccaa ttatccagtt tgtcatataa
     5401 ccaccgtaac ttccccctgc aacccctaag cggtctgcat caatcccagg atatttttca
     5461 atacaagtat ctacaaattc cataagatgc tcatagtcaa cataccccca tctttctacc
     5521 atttctaaga acttttcacc ataaccgtca ccaccatgag ggttacaata aatcacatac
     5581 attccctttg atgttacaca ctgcttcaag tgtgtaaaca tatagccata tgcccattta
     5641 ggcccaccat gaatgaacaa tacagcagga tatttttcgc cttctttagc atctataggt
     5701 ggtataacaa aaccatcaac ttcttcttta ttttttgcta caaacgtcaa tttttcaggt
     5761 aatgaaacct tatgagtatc taaatatggt ttagaaaaag cagtcatttt tttcattgta
     5821 cctgtagtca tatcaatgct atatatttct gctaactcat aatctttcaa cattactgtg
     5881 tacatggtgt tgtcgtaaat atcaaaagat gtaaaagcat ctacttttgt gatgatacgt
     5941 atattacctg tctttgtatc aaactcatta aattctgtca tagttctgca aattcttgca
     6001 ccaataaatt tatctcctat aactttatta ctacgtccac caccatatac tgcatctaca
     6061 tccagcaatg tagcatacat accatctgga ctagcaatca tatttatttc attagtactc
     6121 aaatcaagta taaaaatatc atgattctgt gtgatagaac ggtctaatgt tgaagcttgc
     6181 aacattactt tatctttgcc aatatatttt gccaaactta tttggtactt tccttgtggt
     6241 acaagttctt tggtttcacc actgtctata tgatactccc aaagtccaga aaaaggagtt
     6301 gcacaatctt cataaatctg accagtatat aaaattctgt ttttatcaga agaaacgtct
     6361 acacctgcta cttcacaaaa ttttggtgta atggcttttg ttatattttc accttcttga
     6421 tataattcta atgttacacg tcttctttga gaaaaatttt ctccatctct tataaaaggt
     6481 ttatctgtaa atgtccaata atctacaccc tctactgccc tatctggttc tgatttttta
     6541 gaatccgttg gattttttgt tgtcagtaac cacaatttat ttccaagtga tacaggtacg
     6601 ctacaagctt ttggaataga aaatgctttt tttgcttctc ctccttctat agaaatagtg
     6661 taaaaatctg ttgtacctga ttttggctta tttcttttag atgtaaaaag gagttctgta
     6721 ttagaaatcc aactaaatga tgttacatct tttgtttgag tcatatgtat ttgtttttct
     6781 gttttgatgt ccatcacata taagtcatgt tcatagcagt ctttttcctc gttgatattt
     6841 gatataatat aagcaatatg ctttccatca ggtgaacaag aaatatcatg agggaatata
     6901 tattcaaaaa tatctgaact ttcaatcttt attttttcca tttttatatt ctcctttcta
     6961 ctgtcctatg ttatcttttt ttatatatac cgaaaaccaa tctagcattt caactaatct
     7021 ttgtagcttt tgttttggtc taccagaacg agacagttca tggttttctc cttcaaatag
     7081 acaaagtttt gtaggacacc catgaatttt caatgctgaa aacatctgga atgcttcttg
     7141 catatagcat ctatagtctt tttcaccatg taaaaataaa gtcggtgtaa cacatttatc
     7201 agcataagca agaggtgact gtttccattg aagctcgtgg ttttccatat gagaagcacc
     7261 tgtttgtcct ttgcagaaat aataaccaat gtctgttgta ccctccatgc cactccagtt
     7321 tgcaatagaa cgctgagcac aagcaacagc aaaacggttt gtatgaccaa ttatccaatt
     7381 cgtcataaat ccaccataac ttccaccttc aacagccaat tttgttttat caatgtctgt
     7441 atatttttca agtacagtat ctgtaaattc cataaaatct atataatcaa tatctccaaa
     7501 ctgtttccta atatctgcaa aagcattgcc acgaccttca gacccccttg ggttacagaa
     7561 gaatacaaag taacctgcac ttgtaaatac ctgtatctca tgcataaaat atggtccata
     7621 tgtcaatctt ggtcctccat gaatcgccaa tacagctgga tattttttcc ccacctcata
     7681 atctactggt ttatatacgt atccttgtat ttcccattta gaacttcctt ttgactctat
     7741 gtattctggc acagaaattt tatattcctc taaaatatgg tcatttaaat gtgttttctt
     7801 acacaatgtt ccattctcta ctgtataaac ttctggtaat ccatttccta aacaacccac
     7861 acatacaata ttgccatctt ttacatctat aaaatcaact gaacctgtct gtgttacttt
     7921 ttcctgtttt cctgttggaa tatgtattct ttcaataatg cagtccatat ctactgtaga
     7981 agtgtaataa atatattctc catctacctt tgttgtctga ccagcaccca ttttaacatc
     8041 agatgttact gtatttccaa tacaatgatg ttgatgccct gaaagaagtt tcatttcttt
     8101 cgtatcaata tcaatagtat aaaaatctcc attttctcca tttccataag ataaagcatc
     8161 agttaggttt agtatcactt gattttgata taattcaaaa ccttttataa tatacttatc
     8221 tttttctaat atacagacat ttgtgtttgt gtttgtatca aatacataga ccccattttt
     8281 caaacctttt acatctttaa actcttgccc tatatataag atttttccat cacttatttt
     8341 catacctaca acattaaaca taggtgctgt aattgccttc aactcattta tattactgtc
     8401 gtatatatat aggcgttttc tttttctgct aatatatccc tccccattga cagcataagg
     8461 aatttctttg aaatgttcat aatgtctttt gttataagca tttatttttt tctgttgttc
     8521 ttcttctggc aatccctcta caataggtcg gtcattgtca taagaatcaa taactgcata
     8581 caaacctttt cctataggat tcaatttacc acctttgata gggagtctaa aaagttcttc
     8641 tgcttctcca ccatttaaag ataaagcata gtaactcatg cattgcttac cgtttttgac
     8701 agcttctttt actttttcat ttctcaaagc agggaacaag atagtatttt catcttccca
     8761 ggcatatgct ccaacagagt ttatagacgt tatttgtttt aattcgccag ttttattgtt
     8821 taatatataa acttggcttg tgtaaccatt ctctttgata tcagcttttt ttacaataaa
     8881 tgctgtatat cctccatctg gtgatatttg gcaatcactt ataaattgat attccaaaaa
     8941 tgtttcactc gtaaatagct ttttcatagt attttctgtc ctttctttag taattttttt
     9001 caaactccct accacctctt tcatttttta ttttaaattt aaaataaaaa attttaaatt
     9061 taaaataaaa aattttataa aggttatttt tttaggaagc cttctggctt ccttgtaata
     9121 tttgtaattt aaagtatatt taatccatgc ttaattcata cttaatctat acttaatcta
     9181 tacttaatct atacttaatc tat
//
LOCUS       DAELLV010000146         9192 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.146, whole genome shotgun sequence.
ACCESSION   DAELLV010000146 DAELLV010000000
VERSION     DAELLV010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9192
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.146"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..627)
                     /locus_tag="KPD58_002592"
     CDS             complement(<1..627)
                     /locus_tag="KPD58_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBG0266350.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKL"
     gene            complement(699..1052)
                     /locus_tag="KPD58_002593"
     CDS             complement(699..1052)
                     /locus_tag="KPD58_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
                     /protein_id="HBG0266351.1"
                     /translation="MSTEKRRDNKDRILRTGESQRKDGRYAYKYVYSFGKAQFVYSWK
                     LVPTDKPPKGKRDDISLRDKEKAIQNDLDDSIDTIGKKMTVCQLYAKKNVLRKNIRLN
                     TKKGRHYLMKILNKE"
     gene            complement(1130..1333)
                     /locus_tag="KPD58_002594"
                     /pseudo
     CDS             complement(1130..1333)
                     /locus_tag="KPD58_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
     gene            complement(1723..1953)
                     /locus_tag="KPD58_002595"
     CDS             complement(1723..1953)
                     /locus_tag="KPD58_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0266352.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINVILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            complement(1955..2362)
                     /locus_tag="KPD58_002596"
     CDS             complement(1955..2362)
                     /locus_tag="KPD58_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG0266353.1"
                     /translation="MVDEKTIEHQFDSFCKTVLRNYARDIYDENKRRNDYLVSLESLS
                     LAELSKLSILDDYDSNYICMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFLNMT
                     NADIATLMDLAENTIHYHKTNALKELKELMQEH"
     gene            complement(2723..3161)
                     /locus_tag="KPD58_002597"
                     /pseudo
     CDS             complement(2723..3161)
                     /locus_tag="KPD58_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729090.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(3175..4101)
                     /locus_tag="KPD58_002598"
     CDS             complement(3175..4101)
                     /locus_tag="KPD58_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266354.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNETVEIVDG
                     GFVDWTYQMLGNKKERCLISGIGLERFLVAVS"
     gene            complement(4226..5008)
                     /locus_tag="KPD58_002599"
     CDS             complement(4226..5008)
                     /locus_tag="KPD58_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HBG0266355.1"
                     /translation="MTKTQQEMIQNVLEFSKRDSRIIGLAISGSYITKSLDEYSDLDF
                     LIVADDKSYEEVIEERLLIVEQFGTLVSAFTGEHVGEPRLIISLYDSPVLHVDFKFTT
                     LDGLLDRVEDSAILYEENNCITEIYSKKEAYFPTPDLQWIEDRFWIWVHYTCTKIGRR
                     ELFETIDFLSFLRQSVLASLIQLLLGKLPRGVRKIELDAPQYIEALEKTVANHEYKSC
                     VNALQATINLYLELREELSQSPIVRKVEAERVALQYFQDLIK"
     gene            complement(5164..5784)
                     /locus_tag="KPD58_002600"
     CDS             complement(5164..5784)
                     /locus_tag="KPD58_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG0266356.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            complement(5911..6579)
                     /locus_tag="KPD58_002601"
                     /pseudo
     CDS             complement(5911..6579)
                     /locus_tag="KPD58_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            6671..7486
                     /locus_tag="KPD58_002602"
                     /pseudo
     CDS             6671..7486
                     /locus_tag="KPD58_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897377.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            8157..8924
                     /locus_tag="KPD58_002603"
     CDS             8157..8924
                     /locus_tag="KPD58_002603"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBG0266357.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
BASE COUNT         3085 a         1467 c         1383 g         3257 t
ORIGIN      
        1 taatttatca atatttcctg attgaatatg cttaatgttt gattgaattt ttttatatgg
       61 ccaatcccac catttaattt ctaataattt tgaaattgta tcattattaa atctcttttt
      121 tataattttt gcaggtactc ctccaacaat agaatacggt ggaacgtcat tagtaactac
      181 tgctcttgtt ccaataattg caccatcacc aatatttaca cctgacataa ttatagaatc
      241 ataacctatc cacacatcat ttccaattat aatgttaccc ttattgtccc aagcgtccgt
      301 tatatggttg atatcgagtt tccattcttc ataaaagatg ggaaaagtat atgtggattt
      361 tgatttcatt gagtgatttc cactagtcat tagaaatttt gcattacatg ctattgagca
      421 aaacttacca attattaatt tatcattgtt tatagggtaa tgatacaata cattgttttt
      481 ctcaaaatct cttgggtcat tataaaagtc attatatatt gtgtaatctc caacttctat
      541 attttccctt gttattacat ttttaagata tacagtttga taatcattac ttcttggata
      601 taccttattt gaacttggaa tagtcatatt taaatctctc ctttaatata tattatgttt
      661 ttgactattc cactactaca atacatctct tcacagaatc actccttgtt aagaatcttc
      721 ataagataat ggcgtccttt tttagtgtta agtcggatat tctttcttag tacattcttt
      781 ttagcgtata actgacaaac tgtcattttc ttaccaatag tgtcaatgct atcatcaagg
      841 tcattttgta ttgctttttc cttatccctt aatgatatat catcacgttt tccctttggg
      901 ggtttatcag taggtacaag tttccaagag taaacaaatt gtgctttacc aaatgaatat
      961 acatacttat atgcgtatct tccatctttt ctctggctct ctccagttct taaaattcta
     1021 tctttattat ctcgtctttt ttcagttgac atgataagaa gctcctctca ataaccgaat
     1081 tgagcttgat acgtcatatg aatattataa catataaggc tcaatttcat tagataacgt
     1141 caagtgtatc aacgattttc tcaaattgtt ttctctttat ttgaatgcga ttgctattca
     1201 taatgaccca gccagaatca aggttttcat cagcaagttt acgtaatttt ttctctccaa
     1261 tacgaaagta ttttgacgct tattcaatcg tcaaggtata tttttcccaa ataggtacat
     1321 cagtattgtt catagaatgt atcactcctt tctatgttcg tagttcagaa gtagatagat
     1381 ggctttggta agctccattg gtatttcaac caatcccatt cttatgggca gaaccgtgtc
     1441 atacaggtgt atcattatac tgtggtacag gttacggcaa aacaaccgtt ccaaaggtta
     1501 ttcacaaatc actatatagt agctctcttg attaaggtca tggcgtatag tatttgttgg
     1561 ctatctgtat cacaaacgta tctatctact ttattcagtt gtcaaagaac agtcggcatt
     1621 gatttgccta taaaaaatca caatagctta tgggctttta tagacaaata attagtgatt
     1681 ctccatgtac caaatagctt gatacatgga gagagggcga gattaagaca aattaaatct
     1741 caaaatagca gtgattagtt ttgttcctag cattcgcttt gtttcttctt ctacatgaat
     1801 atagggattt ccgtattcat caaataaagt tttctgtgaa agacgaatga tatagtgttc
     1861 aaaatgttta agtattacgt ttattgcatt tacatcgcca tgtacagcaa gtaaaataat
     1921 tgtaaaaggt aatacttctt tagtaggttt catttcaatg ttcctgcatg agttctttca
     1981 actcttttag tgcatttgtt ttgtgataat gtatggtatt ttctgctaaa tccatcaatg
     2041 ttgctatgtc agcatttgtc atgttgagaa agaatgaaag tagaattata tcttgttttc
     2101 tttttgtaag ttttccgatt gcttgtgcca ttaatacatc ttcaattcgt atgttgtagt
     2161 cgtaagaaac catacagatg tagttagaat cataatcatc taaaatactc aatttactta
     2221 attctgcaag tgacaaagat tcgagagata ccaaataatc atttctgcgt ttattttcgt
     2281 cataaatatc tctagcatag ttacgcaaaa ctgttttaca gaaactgtcg aattgatgtt
     2341 caattgtctt ttcatcaacc ataagatttc accctctttc catcgcaagg agttaaataa
     2401 ccttgcttac tcctatcacg gtgggagggc gttaaatctt cgataaattt caaaaaaaaa
     2461 gagaaaaaaa tacacactct gcctataggg caaaaatgtg tattctttaa aacgtattta
     2521 atttatattg gttgtaagcc accaccatat tgatgtatgg aaatatgcat tggctagctt
     2581 ttcataaaaa tacaaagatg cataagaaat taatagaggg gaaaaactta ttctgttatt
     2641 aaaaagtagg ttaattctaa taaatgctat tgcggtcgaa ttaaactata tacatggcaa
     2701 tcaatcatgt ttctattctt tattatgaat tgtgacagta ttcttcaatc ttaaagccac
     2761 attttacttg actctctatt gtaggaatat acattagcat gaattttgtt aaggttgtat
     2821 ttttcaaagg caattttgca tatttctttt actgcatttg ttgctatccc tttgccccaa
     2881 tagttcctag caagtcagta acctaattca gcatttttat taatagaggt atcttcttca
     2941 aatgttaggc tgatacttcc aatagccctt tcttgataaa aaatagcata gctaagttgt
     3001 ttaacatctg aagttgtcaa acaaaaacta ataaaatctc tagcgttttg ctacctataa
     3061 gggtggggga agttgtcacg aagattgttt gcaatttctt tatcattcgc aaactcaact
     3121 aaatcggtaa tataagcata attccactct ataagttcca tttttcttct ccttttatga
     3181 aacagctaca agaaatcttt ctaagccaat accacttatc aaacagcgtt cttttttatt
     3241 tccaagcatt tgataagtcc agtcaacaaa tccaccgtca actatttcaa cggtttcatt
     3301 tccttgtttg accgataatt tgaaattgat acctttataa taattattat caacatcatc
     3361 attatgaatt gataactcta tattaggaaa aataagtttg atagtttcaa ccattctgtc
     3421 gaaaaatccg tcattatctt tgtatccgtt tcttttgcgt agaataatag agaaacgaac
     3481 attttctatt tcacttaata tatctttata atagtttaag tgcttttcta acaacatttt
     3541 ttcacaagtg taagaccctg tatctatacc agatgaaacc atacaatata agccaaaatg
     3601 tgaatataaa cctttaccta caaaattttg tgctcttatt accctagcag tagtagccat
     3661 gtggataggt atggtattat tttcttcttt tctttttaat ttatcagcaa taattattgc
     3721 caacatattt gaggggtctg caagtgtttc tgtccctcgt agagcactta ctacattata
     3781 ttgatctacg gagccaaaaa cgctacagct acctaatgga gcagagggag aaagcataat
     3841 cgtcttgata tccatattga atgcttttct caataatttt gactccaaag catgaatttc
     3901 ttctggatct ataccgctag ggatagtaaa tctatttagt tggtatgact ttagaatatc
     3961 agttgcagtt aaagtatttg cttgtctatc aaacacttct aatagaagtg agttcaaatc
     4021 tgattttgat agagctgata acttatcaac tatattctta tccccaattt tatttgtaat
     4081 tctacttaaa atatcactca tagttatcct ccatactgtt actttcaaaa taattataga
     4141 attgttacaa caactaatct aggtcatttg accaaaaatg aaaaaacact gaatatttac
     4201 tattcaatat ttttttactt tgggtttatt tgattaaatc ttgaaaatac tgtaatgcaa
     4261 ccctttcagc ttcaaccttt cggacaatag gactttgtga taattcctct cttaattcaa
     4321 gatacaggtt gatagtagcc tgcaatgcat taacacagct tttatattca tggttagcaa
     4381 ccgtcttttc taatgcttca atatattggg gtgcgtccaa ttcaattttt ctaactccac
     4441 gaggtaattt acctaagaga agctgtatta acgaggcaag tactgattgt cttaaaaaag
     4501 ataaaaaatc aattgtttca aatagttctc tacgtccaat cttagtgcaa gtgtaatgaa
     4561 cccaaatcca aaatctatct tcaatccact gtaagtctgg agttgggaag tatgcttctt
     4621 ttttagaata aatttctgtt atgcagttgt tttcttcata gagaatagct gaatcttcaa
     4681 ccctatctag taaaccatct aaggttgtaa acttaaaatc aacatgaaga acaggggaat
     4741 catagaggga aattatcaat cgaggttctc caacatgttc gccagtaaaa gcagaaacta
     4801 atgttccaaa ctgttccaca ataagtaatc tctcttcgat aacttcttca taggatttat
     4861 catctgctac aatcagaaaa tccaaatcag aatattcatc taacgatttt gtgatataag
     4921 aaccgctaat tgcaagtcct attatcctac tatctctttt tgaaaattct aatacgtttt
     4981 gtatcatttc ctgttgtgtt ttagtcataa tataacacct ctcactgtct tttattagct
     5041 atcaatccaa cataattatg cataggagta ttatataatg aagaaataaa aaaaaatagg
     5101 tcacttgacc cgaaataaaa aagtattgaa taattattat ccaatacttt tttgaaatta
     5161 tacttatgtt gtaggaagat tctttaatac ctctcgctga ttaattacac tgaatctttt
     5221 gttttcataa gcaattagat gttcttgtac acattcattt atagcacgat taatgcttct
     5281 aatcggagcg tttacttcgc tagcaagctg ttttttggta agcagattta attcacttct
     5341 ataatagtgt aaggatatac acctcagaag tcgttcttta acacttaaag atgataattc
     5401 ttcaattaat tcagcattta caactaattt actagatatt tctcgaatca gaaattttgt
     5461 agcttcaaaa tctcccctta accagtttaa aaaatcatct ctatgaagtt tatatacatt
     5521 acaatctgtg aaagcgacaa tttcaacggg ttttttccca tcattaaatt gttccatttc
     5581 tccaaaaaaa ctaccacttc tataaagatg aatagttgag atgtttcctt tcacatttaa
     5641 gatgaatgct tctgcaatac cgtcaatcat aagaattaca tattcatttt cttgctcagc
     5701 aaaaagaata gtctctttct ttttatacgt ttttttaaca agcttctttt taatatcttc
     5761 attcataaaa tcaattaaat gcatattcat ctctccttac caaatctttg attaaattat
     5821 atcatctttc aattagttag gtaagatagt aaaaatatat ttacatagaa actattccat
     5881 tattattttc cagttgtcat ttttctgcaa aaacttatca ctgtcacgac tggagatacc
     5941 agtaaggaat gttaggatat caatcgctaa actttcagaa tcctttttac ggctcaaaat
     6001 gacgtaatgg tcaagcaatc ctttgttttc atcatcactg gttaaattga caatgtttat
     6061 ctcatgggct atttctggta gcgtttcttt ccatttgccg cgttttgctt tagggtcaac
     6121 tatgaccgct tgtccgccga atagtaccgc atagtaaaca agcatattgt tactgaataa
     6181 tttaccacca cccaatgaac cgataaacgc agacgctaac gcattggtaa tagaaccctt
     6241 taccccttga ctagcaaggg caggttttag atagacattc tttccagtgt ccagattgta
     6301 gccaagatag atatcctcat gttcgccaag ggtttgtgtc gctccaaaac caagtccagc
     6361 caaaaaatcc gaagtgacat attgaatata atcattgatg taacgtttgc tgacaggaat
     6421 aaattcgcca tgcaatccca acatatcccc aaagggacgc acgagcttga ttgataagtc
     6481 gtcgtaaaag tctttgactt catcacaacg cctttttagc tcgtcaagat cctttgtaga
     6541 tacacggaca acatagctaa gtttatacat agattcttta cagttaaaag tctcttttta
     6601 aaaaaatgta taaattataa atcaaaatat agatactatc aatgacccac taggtgcaaa
     6661 aagcattgat atggttaaat tatcagacgc taaaagctgg gctatccgaa tgagtgaaga
     6721 tgggtgtgct tataaatcca ttgacaatta taagcgctca ctgaaagcgt cgtttttcat
     6781 ggcaattcaa gacgactgta ttagaaagaa tccatttaat ttttagttaa gtgatgtttt
     6841 ggaagataca acagaaccaa aaatcgctct aacaccagaa caggaaatgg gcttattatc
     6901 ctttatgggg agcgataaga cttattataa atattatgat gatgttgtta tcctcttaga
     6961 aacaggacta cgcatttctg aattatgcgg tttgacttta gaattagata tgaaaaataa
     7021 agccattatg gtagaccatc aattgttgag tgatactaag attggctatt atgtagaagt
     7081 tccaaagacc acacagggta agcgagaaat tccaatgact gaaagagctt atcaatcatt
     7141 tgaacgtatc gttgaacggc gtaagaacgc taaagcagaa ccaatcattg ttgatggtta
     7201 ccagaacttc ctatttctta aacaagatgg attaccaaaa caagcaactc attatcaaag
     7261 catgttaaga gcgttagtca agaagtataa caagacccat gaggacaagc taccaaagat
     7321 tacgccacat acctttaggc atacactctg tacaaatatg gctaacaaag gaatgagtac
     7381 taatatattg caatatatta tggggcataa aaacattact atgacgcttg gctattatgc
     7441 acatggttca tttagttctg cgagagctga aatggaacgg ttataatcct agtagtatga
     7501 ctagagttta ctacttattt actacttttg atggcattct tatacggtta aatgccaact
     7561 tatgcaaagt atcttccaaa aagaaaatgt cgatactcct tgtagataca ggatatatcg
     7621 acatttacca acttatgaaa agataatcaa atttaatata agtttttcaa ataaaaagat
     7681 actaaattct ttttagagaa tctatataac attacttaag cttggttggg gtataaacaa
     7741 ttgagtagct acttttgtat ggtgttcaac tttagtcaaa actcttaaat aatgcaaatg
     7801 atttagattc attaatattc aatctcttca taagtagtga aattcgagtt atttctgcat
     7861 aattatacta tgatttatag attaattcta ttagcatcca gaataatatt gtttatatga
     7921 gtaaaattta aagaatattg tgaaaattat agaatgttaa aataaattta ggtaaaaaaa
     7981 agtgtttgta tattatagag taccttttct aaaatatgtg ggattgtttt aagaattaga
     8041 ataaaaatta tttttaaatt agtattttta taatgtatag tatttttaat gtataattaa
     8101 attgtgcaga aaatagaatt taaagtccta tagaatttct gattatggag gagaatatga
     8161 aaagaaaagt tcaagtaaaa aatattacta ttggagaagg tagacctaaa atttgtgtac
     8221 caattattgg gaaaaataaa aaagatatta taaaagaggc taaagagtta aaagatgcat
     8281 gtttggatat tatagagtgg cgagttgatt tttttgagaa tgtagaaaat attaaagaag
     8341 ttaaggaagt tctctatgaa ttaagaagtt atatacatga tatcccttta ttattcacat
     8401 ttagaagtgt agtagaaggg ggagaaaaat taatatcaag agattattat actactttga
     8461 ataaagaaat ttctaataca ggtttagtag atttaataga tgtagagtta tttatgggag
     8521 atgaggtcat agatgaagta gttaattttg ctcataaaaa agaagtgaaa gtaattatat
     8581 ctaatcatga ttttaataaa actccaaaaa aagaagagat tgtatctcgt ttatgtagaa
     8641 tgcaggaatt gggcgcagat ttacccaaaa tagctgttat gccgcaaaat gaaaaagatg
     8701 tgttagtgct acttgaagct acaaatgaaa tgtttaaaat ttatgcagat agaccaataa
     8761 taacaatgtc aatgtcagga atgggtgtta taagtagatt gtgtggtgaa atatttggtt
     8821 ctgcattgac ttttggagct gcaaaaagtg tttcagcacc aggacaaata tcatttaaag
     8881 agttaaattc agtacttaat ctattacata agagtattaa ttaaattaat tatatagaaa
     8941 tattagaagg tattttattt ttgaagatat aaattaaata taaaaaactt ttcagatagt
     9001 aaaatttaaa ttgggcaatt atatctgaaa agcaactatt tagtgtgtat tttgaaagtg
     9061 atatagaaaa tcttataaat caactatggc atatttaaaa tatgccatta aaacctacta
     9121 gacattagat tatagatggt atactagcat ttctattatt tatagatagt aagttttcat
     9181 aataactgtt tt
//
LOCUS       DAELLV010000147         9142 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.147, whole genome shotgun sequence.
ACCESSION   DAELLV010000147 DAELLV010000000
VERSION     DAELLV010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9142
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.147"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            206..526
                     /locus_tag="KPD58_002604"
     CDS             206..526
                     /locus_tag="KPD58_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HBG0266358.1"
                     /translation="MKITLPETAIDTLKSILKDNQDKPNNIRVYFAGVGCGGPSFGLA
                     LDEKKEDDLTYEVGELQFVMSSDEYSQYGDIIIEDTGFGFRVIPENMKDQGGGGCSGC
                     SGCH"
     gene            664..1308
                     /gene="trmB"
                     /locus_tag="KPD58_002605"
     CDS             664..1308
                     /gene="trmB"
                     /locus_tag="KPD58_002605"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBG0266359.1"
                     /translation="MRRRRKKGADEKLLSYTKYVLRDDIDKLKGKWNLKFRNDNPIHV
                     EFGTGKGKFITTLAKQNPDINYIAMELKEEVLLKAVEKADASNLNNILFLWGDVSNIL
                     DYFEAKELSRIYINFCDPWPKNRWSKRRLTHSGFLEMYNRVLEDDGEIHFKTDNEKLF
                     EFSLNEIAANNWLLKNISLDLGNSEYENNVTTEYEDKFMSQGMRIFRCEAKKRN"
     gene            1437..2183
                     /locus_tag="KPD58_002606"
     CDS             1437..2183
                     /locus_tag="KPD58_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266360.1"
                     /translation="MWEGTMNLANLIPFIISAAALSPINSNDKVLSENQLKDIFPDAN
                     LRAVVKRYINPDEMTISNIKALDGEFYATGESISNLKGISYLENVDNFIFWNNNIKEV
                     PKEALSLKDMDSINLANNYLIDDDVVNSLSHNGVDVNCDLNFIDTKDNQYKLDSKYHN
                     VDIAKGESIDLRKIITKKIDSYYKYWEVTDNLPKDLNFIVSVSDKSVLSCEDMIIKGN
                     KTGQAVVKVILSDKNDLNTSQEVLITVNVK"
     gene            complement(2312..2590)
                     /locus_tag="KPD58_002607"
     CDS             complement(2312..2590)
                     /locus_tag="KPD58_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266361.1"
                     /translation="MVDTLEFGLKILFFILSIIWMGKIMILRTDKQIVINPLLIGISA
                     VLVMLHTSQSNIEFFGLDVQYIRIVLYIIYSLIILIGIWATNRRNGIF"
     gene            2865..3311
                     /locus_tag="KPD58_002608"
     CDS             2865..3311
                     /locus_tag="KPD58_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266362.1"
                     /translation="MESKFFNWYTQSLGGTFGIISCICAYLNGYMFVYTNSASKYFES
                     IGFLGIISSYTLLPLCMLTLIFGVIYSYTPETSDKSIFNIPFFTLNKRLILITVVFGF
                     IGARVYFIIPAILITFNLYSNVLIAKLKYILCKNKENDTNLKESEV"
     gene            3581..4033
                     /gene="accB"
                     /locus_tag="KPD58_002609"
     CDS             3581..4033
                     /gene="accB"
                     /locus_tag="KPD58_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBG0266363.1"
                     /translation="MNINEIKELLKAIDSTNLEYVKLESSDLRLEVSKKAQSTSSPVL
                     SVQQESVVDLSLEKPVVNDTPVTSNENLSVVVAPLMGTFYDSPSPDADSFVKVGDVVE
                     EGDTLCILEAMKLMNEITSEIKGEIIEVLVSNEELVEYNQPLFKIKPL"
     gene            4049..5410
                     /gene="accC"
                     /locus_tag="KPD58_002610"
     CDS             4049..5410
                     /gene="accC"
                     /locus_tag="KPD58_002610"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBG0266364.1"
                     /translation="MIKKILVANRGDIAVRIIRTCKELGIKTVAIYSEIDKDCFHRYI
                     ADESICIGPNNISKSYNNIENIIYLALKLKCDAIHPGFGFLSENPEFAKQCEDNNIIF
                     IGPTREQMILMGDKSRARETMMELNIPVVPGSESVLKTKEEALEVAREIGYPVMIKAS
                     SGGGGKGMRIVRKEEELFSNFDMASSEALAAFSNSDLYMEKFIENPRHIEVQVFGDKH
                     SNAIHLGDRDCSMQRRNQKVIEESLSPYLSDEERQKLHKIAVDIVKGVGYIGAGTIEF
                     IVDKDKNFYFIEMNTRIQVEHPVTEMVTNLDLIKLQISIANGDKIPFKQEDITFRGHA
                     IECRINAEDSSKNFAPSPGKIESLNLPGGFGVRFDTFVYAGYTIPPLYDSMIGKLICW
                     AETREECISRIYRALDEIIVEGINTNVEFQKALVTSEEFREDTHHTKFIEDVFMKKEF
                     ATL"
     gene            5425..6279
                     /locus_tag="KPD58_002611"
     CDS             5425..6279
                     /locus_tag="KPD58_002611"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HBG0266365.1"
                     /translation="MIKKFLSSKESKYVTISLDDNFKKNSVDDKFWTYCKGCDSHVFR
                     KDIEENSFVCPKCSRHYGLRARKRINLLIDKGTFMEFNSDVEFQNPLNFPKYKEKVDS
                     YKEKTKESEAVVTGYGRINGIKTVICVMNPDFMMGSMGSIVGEKITYSIEYAAENNLP
                     IIICSASGGARMQEGMVSLMQMAKTSQALSKLEEKSLPYISVLTDPTTGGVTASFAML
                     GDIIISEPNTLIGFAGPRVIEQTINQKLPEGFQTSEFLLEKGFIDMIVDRRKMKEVLY
                     QILAMHKK"
     gene            6300..7244
                     /locus_tag="KPD58_002612"
     CDS             6300..7244
                     /locus_tag="KPD58_002612"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HBG0266366.1"
                     /translation="MIENNSEKIKILENDIKQLITISKANNIDLSDKINSLNEKLEKL
                     KEDAFSHLSAYEKVTLSRDIKRPTTLEYIEHICSNFLELHGDRLFKDDPSMVGGIGQI
                     GKFNVTIVGHQKGRDTKENIKRNFGMPHPEGYRKALRLMKQAEKFNRPIITFIDTSGA
                     FCGLEAEERGQGEAIARNLLEMSKLSVPVITFVIGEGGSGGALGIGVGNDVCMLEHSV
                     YSVISPEGLSSILFKDSSKAKEACDVMKLTSNDLYDLKIIDKIIKEPLGGAQKDVEAV
                     SKEIKAYILERLNHYKDMDKEEIIAQRYKKFRNIGKCL"
     gene            7433..7693
                     /locus_tag="KPD58_002613"
     CDS             7433..7693
                     /locus_tag="KPD58_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HBG0266367.1"
                     /translation="MEVLKVSSKSNPNSVAGALAGVLRERGVAEIQAIGAGALNQAIK
                     SIAIARGFVAPSGMDLVCIPAFTDIEIEGDKKTAIKLIIEPR"
     gene            7846..8217
                     /locus_tag="KPD58_002614"
     CDS             7846..8217
                     /locus_tag="KPD58_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBG0266368.1"
                     /translation="MKLKLLVEEYAVCRLNNDSKIPTWIDTEKFYSITRTDDELSVVC
                     LNNNIPSDVKSEKEWRILKILGPLDFSLVGILSKLSSLLADNKISIFAISTYDTDYIL
                     VKEKDIENACKILSCNGYEVE"
     gene            8247..9092
                     /locus_tag="KPD58_002615"
     CDS             8247..9092
                     /locus_tag="KPD58_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266369.1"
                     /translation="MIYIIEILKISVINIFCFIGIFIVFGLIFSITENLNNKFIFSSF
                     GKTGIIVTGAIGTFVHELSHLIMCLIFMHKINSVKFFRPIESKNDGILGYVSHSYKKD
                     NLYQSIGNFFIGIAPIIGGTIMIILIFKILLPDSYMKVTQNIDLKLYVSMINNLNING
                     FIHIILGDISNFIRIMLLTPSVYSLKYFIFMFLMYSISTHMSLSSADLKNSLNGLSFI
                     VIVIFVISSITYLFGFDNLNISSLIVKYNIFISFFMAIGFIFSIVTLIISYILSLVSP
                     FKNFS"
BASE COUNT         3550 a          950 c         1540 g         3102 t
ORIGIN      
        1 ttttagaata ataattttat ttgtttacta agtttatgat agggtattta tttaaggtaa
       61 taaaatatta tataaaatat tttaaagcct taggtaatat taggtagaag ttttagtaaa
      121 atatgcaaaa actaattgat tatgtacctt gtatgggtat ataatagata ttgttttaat
      181 aaaatatttg aaagggaatg atttgatgaa aattacttta ccagaaacag ctattgatac
      241 tttaaaaagt atcttaaaag acaatcaaga taaacctaat aatataagag tatactttgc
      301 aggtgttggt tgtggaggtc cttcttttgg attagcttta gatgagaaaa aagaagatga
      361 cttaacttat gaagttggag aattacaatt tgtaatgagc tctgatgaat atagtcagta
      421 tggagatata ataatagaag atacaggatt tggatttaga gttattcctg aaaatatgaa
      481 agatcaaggt ggaggaggat gctctggttg ttcagggtgc cactaagatt aagtttttag
      541 taagtttaga actctcttta tagaaataaa tttctatttt aagagagttc tttttatttt
      601 ttggtgagat aatgtataat aaaaatgtat tttactaatt ttttatatgt aaggagaatt
      661 attgtgagaa gaagacgaaa aaaaggtgca gatgaaaagc ttttaagtta tacaaaatat
      721 gttttaagag acgatataga caaattaaaa ggcaagtgga atttaaagtt tagaaatgat
      781 aatccaatac atgttgaatt tgggactgga aaaggtaagt ttataacaac tttagctaag
      841 cagaaccctg acataaatta tattgccatg gaacttaagg aagaagttct tttaaaggct
      901 gttgaaaaag cagatgcttc taatttgaat aatatactat ttttatgggg agatgtaagc
      961 aatattttgg attactttga agctaaagaa ttatcgagaa tttatataaa tttctgtgac
     1021 ccatggccta aaaatagatg gagtaaaaga agactcactc attctggatt tttagaaatg
     1081 tacaataggg ttttggaaga tgatggcgaa attcacttta aaacagataa tgaaaaatta
     1141 ttcgagttta gtttaaatga aattgctgca aataattggc tattaaagaa tatttctctt
     1201 gatttaggaa atagtgagta tgaaaataat gttacaacag agtatgaaga caaatttatg
     1261 tcacaaggta tgcgaatatt tagatgtgaa gctaaaaaac gtaactagta atatgtttat
     1321 aagtttaata gtagaaattt attttaattt atattaataa agtgtacgaa agtaggtata
     1381 tataacaagg aaacagaacc ctctcataga aattaaatta ttataataaa aaatttatgt
     1441 gggagggtac tatgaattta gctaatttaa taccatttat tatttctgca gcagctttaa
     1501 gtccaataaa ttcaaatgat aaggttttga gcgaaaatca acttaaagat atttttcctg
     1561 atgcaaattt aagagcagtt gtaaaaagat atataaatcc agatgagatg actataagca
     1621 atataaaggc tttagatgga gaattttatg caactggtga gagtatttca aatttaaaag
     1681 gtatatcata tcttgaaaat gtagacaatt ttattttttg gaataataat ataaaagagg
     1741 ttcctaagga agctttaagt ctaaaagata tggattcaat aaatcttgcc aataattatt
     1801 tgattgatga cgatgtagta aattctcttt ctcataatgg tgtggatgta aattgcgatt
     1861 taaattttat tgatacaaaa gataatcagt ataaactgga ctctaaatat cataatgttg
     1921 atatagcaaa aggtgaaagt attgatttga gaaaaataat tacgaagaaa attgatagtt
     1981 attataaata ctgggaagtt actgataacc ttccaaaaga tttaaatttt attgtatctg
     2041 taagtgataa gagtgtactt tcatgtgaag atatgataat taaaggtaat aaaacaggtc
     2101 aagcagtggt aaaagttata ctaagcgaca aaaatgattt aaatacatct caagaagttc
     2161 taattactgt caatgtaaaa taatctgtca acttattcaa tcattctatt ttcaatttaa
     2221 gtgtataatc aaaataaagg ttgttagtaa cagaacgcta atattagtga tttgttacta
     2281 gcaaccttta aattttatag aaatagatta tctaaaaaat tccatttctt ctgttagtag
     2341 cccatattcc tattaaaatt atcagtgaat atattatata caaaactatt cttatatatt
     2401 ggacatctaa tccaaaaaat tcaatgttac tctgtgaggt atgcaacatc acaaggactg
     2461 cagatatacc aataagcaaa ggattgatta ctatttgctt atcagttctt aaaatcatta
     2521 ttttacccat ccaaattatt gataatataa aaaataaaat tttaagacca aattctaatg
     2581 tatctaccaa agcgaaaaca tcccttcatt tataataaat ttttcagctc aacaaaatat
     2641 aaaaccctac aaacaacttt tgaatccata ataagataac ttgattaagc actatgaaat
     2701 aagtactata aaacttcatc tacattaata tatactacac tatacttcaa atttcttaaa
     2761 cataaattaa tgaaattaac aaaattttat tcagaaacat ataaagatat attttactat
     2821 ttaaatgcat atcattatat atacagtttt aggaggtgag gtatatggaa tctaaatttt
     2881 ttaattggta cactcaatcc ttagggggaa cttttggcat tatatcatgt atatgcgcat
     2941 atttaaatgg atacatgttt gtttatacaa atagtgctag caaatatttt gaaagtatag
     3001 gatttttggg aataatttca agttatacac tacttccact ttgtatgctt actttgattt
     3061 ttggagttat atattcttat actccagaaa catccgacaa aagtattttt aatatacctt
     3121 tttttacttt aaataaaaga ttaatactta ttacagtagt ttttggtttt ataggtgcta
     3181 gagtttactt tataattcct gcaattctaa taacttttaa cctatatagc aatgttttga
     3241 tagctaaatt aaagtacatc ctatgtaaaa ataaagaaaa tgatactaat ttgaaagaaa
     3301 gcgaagtcta aattgcataa tattggtatt tgtcttaaaa attcccgagg tattaatttg
     3361 tattctcttg tattttgcaa aaaatattga attttattca ttttatactt ataatttata
     3421 ttaataatgg aaaattaaaa caaatataag ttactggttg ttaatttaaa attaaaagat
     3481 atattgatgt gttgattctc tagtcagcac tttttctttt ataaatttgt tttttgtgat
     3541 ataatattag caccaggtaa taatacaagg gggtaacaaa atgaacatca atgaaattaa
     3601 ggaattatta aaggccatag actcaactaa cttagaatat gtaaaacttg aaagtagcga
     3661 tttaagatta gaagtatcta aaaaagctca atctacaagt tcacctgttt taagtgtaca
     3721 acaagaatca gtagtggatc tttctttaga aaagccagtt gtaaacgata cacctgtcac
     3781 atctaatgag aatctatcag tagtagtagc tcctttgatg ggaacattct atgattctcc
     3841 aagtccagat gcagatagct ttgttaaagt tggcgatgta gttgaagaag gcgacacttt
     3901 atgtatatta gaagcaatga agcttatgaa tgagataaca agtgaaatta aaggtgaaat
     3961 aattgaagta ttagttagta atgaagaact agtagaatat aatcaacctt tatttaagat
     4021 aaaaccattg tagtaggagg tatttaagtt gataaaaaaa atactagttg caaatagagg
     4081 cgatatagca gttagaatca tcagaacatg taaagaacta ggtataaaaa ctgttgctat
     4141 ctattctgaa atagataaag attgttttca tagatatata gctgatgaat ctatttgtat
     4201 aggaccaaat aatataagta agagttataa taatatagaa aatataatct atcttgctct
     4261 taaattaaag tgtgatgcta ttcatccagg ctttggattt ttatcagaga atcctgaatt
     4321 tgcaaagcaa tgtgaagata ataatattat ttttataggt ccaacaagag aacaaatgat
     4381 tttgatggga gataaatcta gagcaagaga aactatgatg gaattgaata tccctgttgt
     4441 tcctggttca gaatctgttt tgaaaactaa ggaagaagct ttagaagttg caagagaaat
     4501 tggatatcct gttatgataa aagcctcaag tggaggcggt ggaaaaggta tgagaatcgt
     4561 tagaaaagaa gaagaacttt tttcaaattt tgatatggca agttcagaag ctctagcagc
     4621 attttctaat agtgatttat atatggaaaa gtttatagaa aatcctagac atatagaagt
     4681 acaagtattt ggagataaac attcaaatgc aatacattta ggtgatagag attgttctat
     4741 gcaaaggcga aatcaaaaag taatagaaga atctttaagt ccataccttt cagatgaaga
     4801 aagacaaaaa cttcataaaa ttgcagtaga tatagtaaaa ggagtaggtt atatcggtgc
     4861 aggtactatt gagtttatag ttgacaaaga taaaaacttt tattttatag agatgaatac
     4921 aagaatacaa gttgaacatc cagttacaga gatggtaaca aatcttgacc ttataaaact
     4981 tcaaatatct atagctaatg gggataaaat tccttttaaa caagaagata ttacttttag
     5041 aggtcatgct attgaatgta gaataaatgc agaagattca tcaaagaatt ttgctccatc
     5101 accaggtaag atagaatcat taaatttacc tggaggtttt ggtgttagat ttgatacttt
     5161 tgtatatgca ggatatacta taccaccttt atatgattct atgataggta agttaatatg
     5221 ttgggctgaa actagagaag aatgtataag tagaatatat agagctctag acgaaattat
     5281 tgtagaaggt attaatacaa atgtagaatt tcaaaaggca cttgtaacaa gtgaggagtt
     5341 tagagaagat actcatcata ccaaatttat agaagatgtc tttatgaaga aagaatttgc
     5401 tacattataa ggagtaaata gattatgata aaaaaatttt tatcatcaaa agaatctaag
     5461 tatgttacga tttctttaga tgacaacttt aaaaagaata gtgtagatga taaattttgg
     5521 acctattgca aaggttgtga tagtcatgta tttagaaagg acatagaaga aaattcattt
     5581 gtatgtccaa aatgttcaag acattatggc ttaagagcaa gaaaaagaat aaatttattg
     5641 attgataaag gaacttttat ggagtttaat tctgatgtag aatttcaaaa tccactaaat
     5701 tttcctaaat acaaagaaaa agtagactct tataaggaaa aaacaaaaga gtcagaagct
     5761 gttgtcacag gttatggaag aataaatgga attaaaacag ttatttgtgt aatgaatcct
     5821 gattttatga tgggaagtat gggttctatt gttggggaaa aaataactta ttcaatagag
     5881 tatgctgctg aaaataattt acctataatt atttgttctg cttcaggagg agctagaatg
     5941 caagaaggta tggtatctct tatgcagatg gcaaagactt ctcaagcact ttctaagctt
     6001 gaagaaaaat cattgccata tatatcagtt ctaactgacc ctactactgg aggagtaact
     6061 gctagttttg ctatgcttgg tgatattata atttctgagc caaatacgtt gataggtttt
     6121 gcaggtccaa gagtaataga gcaaacaata aatcaaaaac ttcctgaagg atttcaaact
     6181 tctgagtttt tacttgaaaa aggttttatt gacatgatag tagatagaag aaaaatgaag
     6241 gaagtattat atcaaattct tgctatgcac aagaaatagg gcataaagga ggaactgttt
     6301 tgattgaaaa taatagtgaa aaaataaaaa tcttagaaaa tgatattaaa caattaataa
     6361 ctatttctaa ggcaaataat atagatttaa gtgataaaat aaattcactt aatgagaaat
     6421 tggaaaagct taaggaagat gctttttcac atctatcagc atatgaaaag gtaacactaa
     6481 gtagagatat aaagagacct acgacattgg aatacataga acatatttgt tctaattttt
     6541 tagaacttca tggagatagg ctttttaaag atgacccttc tatggttgga ggaattggtc
     6601 agataggaaa gtttaatgtt actatagttg gacaccaaaa aggaagagat acaaaagaaa
     6661 atataaaaag aaattttgga atgcctcatc cagaaggata cagaaaagca ttaagactta
     6721 tgaaacaagc tgaaaaattt aatagaccta taataacgtt tattgataca tcaggagctt
     6781 tttgtggtct agaagcagaa gaaagaggac aaggagaagc tattgcaaga aacttacttg
     6841 aaatgagtaa actttctgtt ccagtaatca cttttgtaat tggtgaaggt ggtagtggag
     6901 gagctttagg tatcggagta ggaaatgatg tatgtatgtt agaacattca gtttattctg
     6961 taatttctcc agaagggctt tctagtattc tatttaaaga ttcatctaaa gctaaagaag
     7021 cttgtgatgt tatgaagctt acaagtaatg atttatatga tttaaaaata atagataaga
     7081 ttataaaaga acctcttgga ggagcacaaa aagatgtaga agctgtttca aaagaaataa
     7141 aagcttatat tttagaaaga ttaaatcatt acaaagatat ggataaagag gaaattatag
     7201 ctcaaagata taaaaaattt agaaatattg gaaaatgctt atagaatata aagaaatttg
     7261 tagttttttc ataataaaat ggttaaaaaa aataaaggtt ttcttaaaac gattatagaa
     7321 gtatattttt ataaaagtat actttaaatg atgattgtaa ttagtaaatg gcaattatga
     7381 tttagtactt taggtaatgg atactttaac aaattaacaa ggagatgttg aaatggaggt
     7441 actaaaagtt tcatcaaaat ctaatcctaa ttctgtagct ggagccttgg ctggagtact
     7501 aagagaacga ggcgttgcag aaatacaagc tataggagct ggagctctaa atcaagcgat
     7561 aaaatcaatt gcaatagcaa gaggttttgt tgcacctagt ggaatggatt tagtttgcat
     7621 tcccgcattt actgatatag aaatcgaagg tgataagaaa acagccatta aacttataat
     7681 tgaacctaga taatatatct ctttattaga atttaattaa gttttaaagg ttggctatag
     7741 aaataggaca ctatagtgaa ccttttttac tttatatgtt ttattttatg taacttttaa
     7801 tgtataatct taagtaaaca aataagattg gaggaaatat acattatgaa attaaaatta
     7861 ttggtagaag agtatgcagt atgtagatta aataatgaca gtaaaatacc aacatggata
     7921 gatacagaga agttttactc tataactaga actgatgatg aattaagtgt tgtatgttta
     7981 aataataata ttccttctga tgtcaagtca gaaaaggagt ggagaatact taagatacta
     8041 ggtcctttag acttttcact tgtaggaatt ttatctaagt taagtagttt gttagcagac
     8101 aacaaaataa gcatatttgc aatttctacg tatgatacag actatatctt agtaaaagaa
     8161 aaagatatag aaaatgcttg taaaatttta agctgtaatg gatatgaagt agagtagtta
     8221 aatactttta catgaggact taacttatga tttatataat cgaaatttta aaaataagtg
     8281 taataaatat tttttgtttc ataggaatat ttattgtatt tggattgatt tttagcatta
     8341 cagaaaatct aaataataaa tttatatttt catcttttgg aaaaactgga attattgtaa
     8401 ctggagctat aggaacattt gttcatgaat tgagtcattt gataatgtgt ttaattttca
     8461 tgcataaaat aaattcagtt aagtttttca gaccaataga aagtaaaaat gatggtatac
     8521 taggatatgt aagtcatagc tataaaaaag ataatcttta tcaaagtata ggaaatttct
     8581 ttataggaat tgcacctatt ataggtggaa caataatgat tattttaatt tttaagatat
     8641 tactgccaga tagttatatg aaagtgactc aaaatataga tttaaagtta tatgtaagta
     8701 tgattaataa cttaaatata aatggtttta tacacattat tttaggtgat atttccaatt
     8761 ttataagaat aatgttgtta acacctagtg tatattcatt gaagtatttt atatttatgt
     8821 ttttaatgta ttctatatct acacatatgt cacttagtag tgctgattta aaaaatagct
     8881 taaatggtct gagctttata gttatagtta tttttgtaat aagttcaatt acatacttat
     8941 ttggatttga taacttgaat atatccagtt taatagttaa gtataacata tttataagtt
     9001 tctttatggc aatagggttt attttttcaa tagtaactct cataatatcc tatattttaa
     9061 gcttagtcag cccatttaaa aactttagtt aaataaatac atattttact aatatttggt
     9121 ttcgttagaa acatataaaa at
//
LOCUS       DAELLV010000148         9120 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.148, whole genome shotgun sequence.
ACCESSION   DAELLV010000148 DAELLV010000000
VERSION     DAELLV010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9120
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.148"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            160..1575
                     /locus_tag="KPD58_002616"
     CDS             160..1575
                     /locus_tag="KPD58_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG0266370.1"
                     /translation="MWITINRNSNISLSRQIYCEIKDMILDGSLKFEQKLPSSRTLSK
                     ELSVSRNTVLDAYNQLIAEGYLETRNGYGTIVAKGISGHKVDFKLNDTFRDEKDLHTN
                     KQKNYIDFRSGVPELKLFPRKELSKLYKQILYDLSDSDFRYGSTAGVWVLRVEISKYL
                     FRTRGIRCNPKNIMIVSGSTQGLSLISQLLYKSNKKVAVEDPIHKGLLKVISSIGYSV
                     VGIKVDDKGMNTNLIKSIKGISFVYTTPSHQYPLGGILPIQRRLELIKCATENDYYII
                     EDDYDSEFRYEGQPISSLYELSPNKVIYIGSFSKILSPAIRVGFMLLPDNILESYKTL
                     KMYSDVHTETILQHVLAEFIGNGGLEKYIFRMKKLYNRKRKYLIKVLNTEFKNEFKIK
                     GEAAGLHIVAHFYNVKFTESLIHKIFEAGIKVYPIQQYAIENILEYDYEIILGYAHLS
                     FDEIEKGVKILSKIIHMNQGT"
     gene            1798..2256
                     /locus_tag="KPD58_002617"
     CDS             1798..2256
                     /locus_tag="KPD58_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0266371.1"
                     /translation="MEIIDYIEENLTENLNLDKIAYIANYSKYHLHRMFTNVVGCTIH
                     QYIQRRRLTEAARQLIFTDKYIIDIAITVGYSTHQSFTLAFRNLYKESPQVYRNKDEF
                     YPIQQRFVLNLNHQIQSEKCISYANMNYKQRRDEINSYLNFNINCEVKVA"
     gene            2253..2837
                     /gene="clpP"
                     /locus_tag="KPD58_002618"
     CDS             2253..2837
                     /gene="clpP"
                     /locus_tag="KPD58_002618"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBG0266372.1"
                     /translation="MSLVPHVIEQTGQGERSYDIYSKLLKDRIIFIGEEINDAIASLV
                     VAQLLFLESEDPDSDIIIYINSPGGSATSGFAIYDTMNYVRCDVSTICIGMAASMSAF
                     LLAGGTHGKRCALPNSEIMIHQPMGGAKGQATDVKIAVDNILKIKERLDKILSENTGK
                     SIEDIRRDTDRDNFMTALEAKEYGLIDYIMNRNE"
     gene            3149..4225
                     /locus_tag="KPD58_002619"
     CDS             3149..4225
                     /locus_tag="KPD58_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG0266373.1"
                     /translation="MITISLCMIVKNEEEVIGRCLECVKDIVDEIIIVDTGSTDSTKE
                     IVSKYTDMVYDFEWIDDFSAARNFSFSKASKDYTMWLDADDIILEADREKLLKIKESL
                     DTSIDIVMAKYNVSFDENGNPTLSYYRERLFKRSSNHKWVDPIHEVVPLFGEVFYSDI
                     AISHKKLHRQDPLRNLRIFEKMISEGKTLEPRHQFYYSRELYYNARYKEAIEGFTKFL
                     DSSRGWIEDCISACRDLATCYYLINDEKSALYSLFRSFEFDEPRAEICCDIGKHMFDR
                     QKYKEAIFWYKVALTRDKNDTNGGFKSNDCYGYIPSIQLSVCYDRLGESDKAIYYHEK
                     TKEIKPNDSAVLHNENYFSKFKNS"
     gene            4292..6358
                     /gene="bclA3"
                     /locus_tag="KPD58_002620"
     CDS             4292..6358
                     /gene="bclA3"
                     /locus_tag="KPD58_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA3"
                     /protein_id="HBG0266374.1"
                     /translation="MLLIMSRNKYFGPFDDNDYNNGYDKYDDCNNGRDDYNSCDCHHC
                     CPPSCVGPTGPMGPRGRTGPTGPTGPTGPGVGATGPTGPTGPTGPTGNTGNTGATGLR
                     GPTGATGATGPTGAIGFGVTGPTGPTGATGATGADGVTGPTGPTGATGADGITGPTGA
                     TGATGFGVTGPTGPTGATGVGVTGATGLIGPTGATGTPGATGPTGAVGATGIGITGPT
                     GPTGATGADGATGVTGPTGATGADGVTGPTGATGATGFGVTGPTGPTGATGIGITGAT
                     GLIGPTGATGATGATGPTGVTGATGAAGLIGPTGATGATGADGATGATGATGATGPTG
                     ADGLVGPTGATGATGADGLVGPTGPTGATGVGITGATGATGATGPTGADGLVGPTGAT
                     GATGADGVAGPTGATGATGNTGADGATGPTGATGPTGADGLVGPTGATGATGLAGATG
                     ATGPIGATGPTGADGATGATGATGPTGPTGADGLVGPTGATGATGATGATGPTGATGA
                     TGATGPTGASAIIPFASGIPLSLTTIAGGLVGTPGFVGFGSSAPGLSIVGGVIDLTNA
                     AGTLTNFAFSMPRAGTITSISAYFSTTAALSLVGSTVTITATLYQSTAPNNSFTAVPG
                     ATVTLAPPLTGILSVGSISSGIVTGLNIAATAETRFLLVFTATASGLSLVNTVAGYAS
                     AGIAIN"
     gene            6527..7288
                     /locus_tag="KPD58_002621"
     CDS             6527..7288
                     /locus_tag="KPD58_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HBG0266375.1"
                     /translation="MKNKIEISMNNFVKNYHKSNNITTKWQELLFAYASAEDDMFQKL
                     KSVVGTSHAMPKDFLENGKTIITYFIPFDESVNNSNIGGFECSEIWARAYIETNMLIS
                     NLNDFLCEELEKMGANSYAIPGSYNFDSEKLVSDWSQRHVAFIAGLGTFGLNNMLITE
                     KGCCGRIGSIITSLELEPTKRPENEYCLYKHNNSCKKCVNKCVKEALKEDGFNRNKCY
                     EMCMINEKKYTYIGGCDICGKCIVGVPCSVINPSR"
     gene            7597..7875
                     /locus_tag="KPD58_002622"
     CDS             7597..7875
                     /locus_tag="KPD58_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266376.1"
                     /translation="MSYKKAIHILPEELLELIQEYVDGECIYIPRKSNNKKEWGSNTP
                     TREELSIRNMQIYEDYQVGYDLQYLSEKYYLSLKSIQRIVLQEKRKDI"
     gene            8168..8743
                     /locus_tag="KPD58_002623"
     CDS             8168..8743
                     /locus_tag="KPD58_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1062 domain-containing protein"
                     /protein_id="HBG0266377.1"
                     /translation="MKKITWEVEYISQLPVIRYCKKCGKKTEYICSGLFRVNAQHKYL
                     DIWLIYKCSNCDSTWNSTIYSRVNPNRLSTETLEQFHTNDDNLANQYAMNVELLHRNG
                     AEVGIPEYKIVGQEIDINDLTQVRIVSKYPSQLKVSALLREKLGLSRKAFDEMLDCGT
                     IKSISGVDLRRSKLNYEIVLEVGKQKQKSSN"
BASE COUNT         3514 a         1189 c         1751 g         2666 t
ORIGIN      
        1 aatattggga taaatttctt taactttatg tgatacctct aacataatat agactccttt
       61 ttattttata ttttatgata taatcttagc aaattaaaag tggattttat agttccattt
      121 ttatagtaat tttatagaac cacttttgga ggtattagaa tgtggattac gattaataga
      181 aatagtaata tatcattatc aagacaaatt tattgtgaga taaaagatat gattctggat
      241 ggaagcttaa agtttgagca aaaactgcca tcatctagga ctttatcaaa agaactatct
      301 gtatctcgta atacagtttt agatgcatat aatcaattaa ttgcagaagg atatttggaa
      361 acaaggaatg gatatggaac aattgtagct aaaggaatat cagggcataa agttgatttt
      421 aagttaaatg atacttttag agatgaaaaa gatttacata ctaataaaca aaaaaattat
      481 atagattttc gttcaggagt tccagaatta aaactatttc cacgcaaaga actttcaaag
      541 ctatataaac aaatactgta tgatttatca gattcggatt tcagatatgg aagtacagct
      601 ggtgtatggg ttttaagagt ggaaatatct aagtatttat ttcgtactag aggaattaga
      661 tgtaatccca aaaacattat gattgtttct ggttcaacac aaggtttatc tttaatttca
      721 cagcttttat acaaaagtaa taaaaaggtt gctgtagaag accctattca taaaggctta
      781 ttaaaggtta tttcatctat tggatattca gttgttggaa ttaaagtaga tgataaggga
      841 atgaatacca acttaataaa atcaataaag gggatttctt ttgtttatac tacaccttct
      901 catcaatacc cattaggagg gattttacct attcaaagaa gactagagct tattaaatgt
      961 gcaacagaga atgattacta tataattgaa gatgattatg atagtgaatt tagatatgag
     1021 ggtcaaccaa ttagttctct ttatgaactt agcccaaata aagtaattta cataggttct
     1081 tttagtaaaa tattatctcc agctattcgt gttggattta tgttattacc agacaatatt
     1141 ttagaaagct ataaaacctt aaaaatgtac tctgatgttc atacagaaac aatattgcaa
     1201 catgtattag ctgaatttat tggaaatgga ggtttagaga agtacatatt tagaatgaag
     1261 aagttgtaca atagaaaacg taagtacctc ataaaagttt taaacacgga atttaaaaat
     1321 gaatttaaaa taaaaggtga agcagcaggg cttcatatag ttgctcattt ctataatgtt
     1381 aaatttacgg aaagtttaat tcataaaatt tttgaagcag gtataaaagt ttatcctata
     1441 caacaatatg caattgagaa tatattagaa tatgactatg aaattatact aggttatgct
     1501 catcttagtt ttgatgaaat tgaaaaaggt gtaaaaatac tcagcaaaat aatacatatg
     1561 aatcaaggta catagataaa acatgtagca atctacatat aatagatgta attaagttta
     1621 aactttaaaa gaaatatata ttaatatttt tgtataaaaa tacctgatat ttttttatcg
     1681 atactttatt gctatacact aaattaagat agcaatttat attaagaaaa aactttttgc
     1741 ataggttata atacttaaaa ggagaggata gagttgaaaa caaatacaga tgttattatg
     1801 gagataattg attatataga agaaaatctt acagaaaatc taaatttaga taaaatagct
     1861 tatattgcta attattcaaa ataccattta cacagaatgt ttacaaatgt tgtaggatgt
     1921 actatccatc aatacataca gaggaggaga cttactgaag cagctagaca acttatattt
     1981 acagacaaat atattataga catagctatt acagtaggat attctacaca tcaatctttt
     2041 acactagcat ttagaaattt atataaagaa tctccacaag tatatagaaa taaagatgaa
     2101 ttttatccta ttcaacagag atttgtttta aatctaaatc atcaaattca aagtgaaaaa
     2161 tgtatttctt atgcaaatat gaattataaa caaagaagag atgaaatcaa ttcttatctt
     2221 aactttaata ttaattgtga ggtgaaagta gcatgagttt agtgccacat gtaattgaac
     2281 aaacaggtca aggggaaagg tcttatgata tatattcaaa attattaaaa gatagaatta
     2341 tatttatagg agaagaaatt aatgatgcta ttgcaagttt agtagtagct caattactgt
     2401 ttttagaatc agaagaccca gattcagata taattatata tataaatagc cctggtggct
     2461 ctgctacatc aggatttgct atatatgata ctatgaatta tgtcagatgt gatgtatcga
     2521 ctatttgtat tggtatggct gcaagcatga gtgctttttt acttgcagga ggaacacatg
     2581 gaaaaagatg tgctttacct aattcagaga ttatgattca tcagccaatg ggaggagcta
     2641 aaggtcaagc aacagatgta aaaatagctg tagataatat attgaaaata aaagaacgat
     2701 tagataaaat tctttctgaa aatacaggta aatcaataga agatattagg agagatacag
     2761 atagagataa ttttatgact gcactagagg ctaaagagta cgggctgatt gattatataa
     2821 tgaataggaa tgaataaagt atcaaagtat catattaaaa tgtctgtttt acaaatatat
     2881 atataatatt aaataagagt gtctcaaaat gaacttttta gtttatggga cactcttttt
     2941 tgtatgaaat taaatatggt tttatcattt caacaattat taccaaattt caagtttaat
     3001 aaattctact ataaaaaata cataaaagat aaggttcttt agtaactttc tgtcgtcaaa
     3061 agtttttata aatttaataa caaaacaaga gaaaatacca tatattactt atgtctgcaa
     3121 tatatagaag acaaaggagt tgtacattat gattacaata agtttgtgca tgattgttaa
     3181 gaatgaggaa gaagttatag gcagatgctt agaatgtgta aaggatatag tagacgaaat
     3241 tataattgta gatacaggtt ctacagattc cacaaaagaa atagtctcaa aatatacaga
     3301 catggtgtac gatttcgaat ggatagatga tttctctgct gcaagaaatt tttcattttc
     3361 taaagcttct aaagactata ctatgtggct tgatgcagat gacataattt tagaagctga
     3421 tagagaaaaa ttattaaaga ttaaagaaag cttggatact tccattgata ttgttatggc
     3481 aaaatacaat gtatcctttg atgaaaatgg taacccaact ctctcgtatt atagagaacg
     3541 attgtttaaa agaagttcta atcataaatg ggttgaccct attcatgaag tagtacctct
     3601 ttttggagag gtattttatt ctgacattgc aatatctcat aaaaagcttc atagacagga
     3661 ccctctaaga aatcttagaa tttttgaaaa aatgatttca gaaggaaaaa cattggagcc
     3721 aagacaccaa ttctattatt ctagagagtt gtactataat gctaggtata aagaagcaat
     3781 agaaggtttt actaagtttt tagactcatc aagaggatgg attgaggatt gtatcagtgc
     3841 ttgcagagat ttagccacat gttattactt aattaatgat gaaaaaagtg ccttatattc
     3901 attgtttaga agtttcgaat ttgatgagcc aagagcagaa atttgttgtg acataggaaa
     3961 gcatatgttt gatagacaaa aatataaaga agccattttt tggtataagg tagcattaac
     4021 aagagataag aatgatacta atggaggttt taagtcaaat gattgttatg gatatatacc
     4081 atctatacaa ttaagtgtct gttatgacag gttaggagaa tcagataaag ctatttatta
     4141 tcatgaaaaa acaaaagaaa tcaaacctaa cgatagtgct gttttgcaca atgaaaatta
     4201 ttttagtaaa tttaaaaata gttgataggt cacaatcata ctaaatttga catattatat
     4261 atagagctta aaagctcaaa tatatcagga ggtgctttta ataatgagta gaaataaata
     4321 ttttggacca tttgatgata atgattacaa caatggctat gataaatatg atgattgtaa
     4381 caatggtcgt gatgattata atagctgtga ttgccatcat tgctgtccac catcatgtgt
     4441 aggtccaaca ggcccaatgg gtccaagagg tagaaccggc ccaacaggtc caacgggtcc
     4501 aacaggtcca ggagtagggg caacaggccc aacaggacca accggtccga ctggtccaac
     4561 aggaaataca gggaatacag gagcaacagg attaagaggt ccaacaggag caacaggggc
     4621 aacaggccca acaggagcta tagggtttgg agtaacaggt ccaacaggcc caacaggagc
     4681 gacaggagca acaggagcag atggagtaac aggtccaaca ggtccaacgg gagcaacagg
     4741 agcagatgga ataacaggtc caacaggagc aacaggggca acaggatttg gagtaacagg
     4801 tccaacaggc ccaacaggag caacaggagt aggagtaaca ggagcaacag gattaatagg
     4861 tccaacagga gcaacaggaa cacctggagc gacaggccca acaggagcag taggagcaac
     4921 aggaatagga ataacaggtc caacaggtcc aacaggagca acaggagcag acggagcaac
     4981 aggagtaaca ggtccaacag gggcaacagg agcagatgga gtaacaggtc caacaggggc
     5041 aacaggagca acaggatttg gagtgacagg cccaacaggt ccaacaggag caacaggaat
     5101 aggaataaca ggagcaacag ggttaatagg tccaaccgga gcaacgggag caacaggagc
     5161 aacaggccca acaggagtaa caggggcaac aggagcagca ggactaatag gaccaaccgg
     5221 ggcaacagga gcaaccggag cagatggagc aaccggagcg acaggggcaa caggagcaac
     5281 aggtccaaca ggagcagatg gattagtagg tccaacagga gcaacaggag caacaggagc
     5341 agatggatta gtaggcccaa caggtccaac aggggcaaca ggagtaggaa taacaggagc
     5401 aacaggagca acaggagcga caggtccaac aggggcagat ggattagtag gtccaacagg
     5461 agcgacggga gcaacaggag cagatggagt agcaggtcca acaggagcaa caggagcaac
     5521 aggaaataca ggagcagatg gagcaacagg tccaacaggg gcaacaggtc caacaggagc
     5581 agacggatta gtaggtccaa caggagcaac aggagcaaca ggattagcag gagcaacagg
     5641 agcaacaggc ccaataggag caacaggtcc aacaggagca gatggagcaa caggggcaac
     5701 aggagcaaca ggtccaacag gtccaacagg ggcagatgga ttagtaggtc caacaggagc
     5761 aaccggagca accggagcga ccggagcaac aggtccaaca ggagcaacag gagcaacagg
     5821 agcaacaggt ccaacaggtg ctagtgcaat aatacctttt gcatcaggta taccactatc
     5881 acttacaact atagctggag gattagtagg tacaccaggt tttgttggct ttggtagttc
     5941 agctccagga ttaagtatag ttggtggagt aatagacctt acaaacgcag caggaacatt
     6001 gactaacttt gcattttcaa tgccaagagc tggaacaata acatctattt cagcatactt
     6061 cagtacaaca gcagcacttt cacttgttgg ctcaacagtt acaattacag caacacttta
     6121 ccaatctact gcaccaaata actcatttac agctgtacca ggagcgacag ttacactagc
     6181 tccaccactt acaggaatat tatcagttgg ttcaatttct agtggaattg taacaggatt
     6241 aaatatagca gcaacagcag aaactcgatt cttactagta tttactgcaa cagcttcagg
     6301 tctttcatta gttaatactg tagcaggata tgcaagtgca ggaattgcaa taaattagat
     6361 tagataagaa aaggcttcta atatcgtatt gatattagga gcttttttaa gaatgattga
     6421 tgaacatagt tttaatatta attagtgcgc atattataaa atactattat ataatataaa
     6481 ataataacat taaattggaa atatagaatt atctaggggt gatactatga aaaataagat
     6541 tgaaatatca atgaacaact ttgtaaaaaa ttatcataaa tcaaataata taactacaaa
     6601 gtggcaagaa cttttgtttg cttatgcatc tgctgaagat gatatgtttc agaagctaaa
     6661 aagtgttgta ggaacatctc atgctatgcc aaaagatttt ctggaaaatg gaaaaactat
     6721 aattacatat tttatacctt ttgatgaaag tgtaaataat agtaatattg gagggtttga
     6781 atgttctgaa atatgggcaa gagcgtacat agaaactaat atgttgattt caaatttaaa
     6841 tgatttctta tgtgaagaac tagagaagat gggggctaat tcatatgcta ttcctgggtc
     6901 gtataatttt gacagtgaaa aattggtaag tgactggtca caaagacatg tagcattcat
     6961 agcaggtctt ggaacttttg gtttaaataa tatgcttatt acagaaaaag ggtgttgtgg
     7021 aagaatagga agcataatca caagtttaga attagagcca actaaaagac cagaaaatga
     7081 atactgcttg tataaacata ataatagttg caaaaaatgt gtcaacaaat gcgtgaaaga
     7141 agctttaaaa gaagatggat ttaataggaa taagtgttat gaaatgtgta tgataaatga
     7201 aaaaaaatac acttatatag gtggatgtga tatttgtgga aagtgtatag ttggagttcc
     7261 ttgttcagta ataaacccat caagataatg gatttaagtt gactaataat tttaaagata
     7321 gtttataatg aaaaaatttt agttatcaaa ataaatataa tataaaaact aaataaatgt
     7381 gtttaaaaaa tggataaata atttgtgttg attgacaacc agttatactg ttgatagact
     7441 tatatgtatc gctgtgatat ataaggaggg ttgagatttt atgtttgatt taatctttat
     7501 ttttgcatag caagggctat tgcaactatg aaatttaata tataaatagc cctttgctat
     7561 tcctaatgaa aaattactat ttaataggag agcaaaatga gctataaaaa agcaatacat
     7621 atattaccag aagaattact tgaattaata caggaatatg tagatggtga atgtatctat
     7681 attcctagaa aatctaataa taaaaaagaa tggggaagta atacacctac tcgtgaggag
     7741 ttgtcaatta gaaatatgca gatttacgaa gactatcaag ttggatatga tttgcaatat
     7801 ttatcggaaa agtattattt atctttgaaa agtatccaaa gaattgtatt acaagaaaaa
     7861 agaaaagata tctaaggatg agccagtata gttttataat tatactggct cattttcttt
     7921 tgataaaagt gtttgaggta tagtttcttt taagtggatg atataattta agtaatttca
     7981 ataacttata cagtagctaa caaagctggt tgtaaagtat ctaataagaa gtagtagctt
     8041 tttatatgtt tgaaattaaa atttattaaa attaggaggt cttgttttga aaaaaatact
     8101 tgattatatt ccaccttttc ataactttct attaatttag taatcttata gaaaggaaat
     8161 tgcatttatg aaaaaaatta cttgggaggt ggaatatatc tcccagttac ctgtaattag
     8221 gtattgtaaa aaatgtggaa aaaagactga atatatttgt tcaggtttat ttagggttaa
     8281 tgctcaacat aaatatttgg atatatggtt aatatacaag tgttcaaatt gtgattcaac
     8341 atggaactca actatatatt ctcgtgttaa tccaaataga ttaagtacag aaacactaga
     8401 acaattccat acaaatgatg ataatcttgc taatcaatat gctatgaatg tagaactttt
     8461 acatagaaat ggagcagagg ttggtattcc agaatataaa atagtaggac aagaaattga
     8521 tattaatgac cttacacaag ttcgtattgt aagtaagtat ccatcccaat taaaggtgtc
     8581 agcattatta cgtgaaaaac taggtttatc taggaaagca tttgatgaaa tgttagattg
     8641 tgggactatt aaaagtatat ctggagttga tttaaggaga agtaaattaa attatgaaat
     8701 tgtgttagag gtaggcaagc agaagcaaaa gtcttcaaac tgattagaat aactaactta
     8761 atatattttc aggaacatca tttaaaaaaa tagtgacttt aaaaaattta aagccactat
     8821 tttttaatat gttttatatg agtatttaaa ataaagtgtg tattcttcaa atttatagcg
     8881 ataattaaaa cattactata aaaaggaagg tgcatacttt ttatttaaca aaaactagat
     8941 attaaaaact tttcgaggaa tattttatta aaatccctaa atatatttaa tatatattaa
     9001 aaaattgtat tataatgaaa atcaaaatag attgcaaatc caaaaagtaa tacttaatgt
     9061 ccaaaaagct aaaattaaaa tctaaatagc tatttactac agaagtatgt taaaatatgt
//
LOCUS       DAELLV010000149         8996 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.149, whole genome shotgun sequence.
ACCESSION   DAELLV010000149 DAELLV010000000
VERSION     DAELLV010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8996
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.149"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            32..472
                     /locus_tag="KPD58_002624"
     CDS             32..472
                     /locus_tag="KPD58_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0266378.1"
                     /translation="MFKIKHFNDLSLDEFYEIAKSRYEVFACEQKIFSLNDYDDIDKS
                     SYHIFLKENGLICAYARIIPKEYSSYNDVSIGRVLVLSSHRRKGLAKQMMDCAIDFIK
                     VNLHENNITLSAQTYIKNLYLSCGFKEISEVYDEAGIEHIKMRL"
     gene            799..1026
                     /locus_tag="KPD58_002625"
     CDS             799..1026
                     /locus_tag="KPD58_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG0266379.1"
                     /translation="MKNLNDIQVKKTVKVEDILSSGNLRERMLALGLTRGAVIDVVRK
                     GPKNNLTVYKIRGSKIALRQEESSLILVSDI"
     gene            1052..3172
                     /gene="feoB"
                     /locus_tag="KPD58_002626"
     CDS             1052..3172
                     /gene="feoB"
                     /locus_tag="KPD58_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBG0266380.1"
                     /translation="MGLTHNSTKMSSLKDMFDIDNKEDQFVIALAGNPNTGKSTVFNH
                     LTGLRQHTGNWPGKTVATARGNFKYKNTEYALIDLPGTYSLFALSQEEIVARDFICFG
                     NPDAVIVVCDATCLERNLNLVFQVMELTDKVILCINLIDEARKKGITIDKKLLEDSLG
                     IPVILTAARNGSGMDELLDTLNDVSFDKYKLNNKPVRYNENIENVVKSIQPELDNIIP
                     GINSRWLGLRLIDGDESIFESMSNYIDKDSIDAINEVKKKIPDNINKQKIRDEFTKIN
                     YDYAKKLSDECCSNVAKKSTDREEKVDKILTSKIFGLPIMLLLLGTILWITIEGANYP
                     STLLSNLLLGFEPSISGILNSINCPSWLNDMLVLGLYRTLAWVISVMLPPMAIFFPLF
                     TLLEDFGYLPRVAFNLDHLFKKACAHGKQCLTMCMGFGCNAAGVIGCRIIDSPRERLI
                     AILTNNFVPCNGRFPTLIAISTIFFSSVITNSFVSSVATALCITLLIILGVIITLLVS
                     YTLSKTLLKGVPSTFTLELPPYRVPQIGRTLYTSIIDRTIFVLGRAVMVAIPAGVITW
                     IFANIYIGDLSILSHVANFLDPLAKLIGLDGFILLAFILGFPANEIVVPILLMAYLAT
                     GSMIELDSFSALGQVLREHGWTYLTALNVMLFSLLHWPCATTLLTIKKETGSLKWTAL
                     GFLMPTILAFVVCFLTTTVYNLFI"
     gene            complement(3305..4123)
                     /locus_tag="KPD58_002627"
     CDS             complement(3305..4123)
                     /locus_tag="KPD58_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0266381.1"
                     /translation="MEKQYFTTGEFAKLCGISKQTLIFYDKIGIFSPEYKDKNNYRYY
                     SVYQYDTLDILQSLREIGMSLEEIKEYIQNRTPQLCVKMLKEEEKKIREKIKKLRKIS
                     SKMQNRINITVEGISKMNSEEIYISKKFEEYLVLSSDLSNMSNSDFMMELIDFTNYCK
                     SKNLYQGYELGVIVSNENIKNGDYLSISSFYLKIDKKIKDKKLYVKPEGMYACIKHIG
                     KYEDSYKSYEKLKKYIYENEYKIVGNSYEESILDFFCESNEDNYMTEISIQVSR"
     gene            4330..4614
                     /locus_tag="KPD58_002628"
                     /pseudo
     CDS             4330..4614
                     /locus_tag="KPD58_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889354.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     repeat_region   4782..5871
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=4849..4877
                     /rpt_unit_seq="atttacattccatatagttaatataaaac"
     gene            complement(6530..8623)
                     /locus_tag="KPD58_002629"
     CDS             complement(6530..8623)
                     /locus_tag="KPD58_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG0266382.1"
                     /translation="MNKHNFEVILNQLQINIYVTNIHTNEIIFMNKKMKEEYNILDPE
                     GKVCWQVLYPEKNSTCSFCKVLELLKNDKKGVLIKWYEKCNKLNRVFENYDSLITWQD
                     GTVVHMHQSIDIANSTSLNKPIKINEFHEISNNKEEKGVFNFSRDNFDYNSTLLYDAL
                     IRGTDEYIYICNMKTGVFRYSPSQVELFDLPGEIVENPLVYWKKIVHPEDWNRFYKSN
                     TEIGKNQMDYHTVEFRAKNRSGEYIWLRCRGQLMRDEFGEPSIFAGIMTQLGKQNKID
                     SLTQLLNYHEFMSVFEDKISNPMIEKLCIVLLNIDDFKNVNEMYDRDFGDNIIKTLAQ
                     SIQSILPDNAELYKLDGDEMGILVDNVEENEILTLYNQIQNMIIHLQLWRKYGLNITI
                     SAGCVIYPKHGDTVKELYKCASYSLQYAKEHGKNRLVFFSQEILKNKMYSLEMMRDLK
                     ASINDDFRGFSLRFQPQVDTESHKIIGVEVLLRWTNDKCKAISPLEFIPILEENDMIN
                     IVGAWVLRMALRTFRKWIDYYPFFKVSVNVSAVQILEDTFIEDIVKIIDDENFPYQNL
                     VLELTESHTVQNMSILQFKFKVLQDLGIYIAMDDFGTGYSSLEVLKFSPIDIVKIDRV
                     FVKDILKSKFDATFIHFIVAICHDVGIKVCLEGVETQEEYDLVKQIKPDYIQGYLFGK
                     PQTATEIFDLLKLDN"
BASE COUNT         3245 a         1237 c         1100 g         3414 t
ORIGIN      
        1 cagtttttta agatgctagg aggtaattac tatgtttaaa attaaacact ttaatgattt
       61 gagtttagat gaattttatg aaatagcaaa atcaagatat gaggtatttg cttgtgaaca
      121 gaagatattt tcattaaacg attatgatga tatagataaa agctcttatc acatattttt
      181 aaaagaaaat ggattgattt gtgcttacgc tagaataatt cctaaagaat acagctctta
      241 taatgatgtt tccattggta gagttttagt tctttctagt catagaagaa aaggattagc
      301 aaaacaaatg atggattgtg ctatagattt tataaaagtt aacttacacg aaaataatat
      361 aaccttatca gcacaaactt atataaagaa cctttactta tcttgtggtt ttaaagaaat
      421 ctcagaagta tacgacgaag caggtattga acatattaaa atgagattat agtgttgaca
      481 agctaatatt gttaatatat aatttaagta taaattaaat attgaattga tatttaggta
      541 tttgaaaata ccttacatag agtcagaagg cttttgcttt ctgacttttt tattatcaaa
      601 attcaataat tatatggatt caaagtgtta atttttattc tatattctac tttccccaaa
      661 agtaagaatt atacccaact ttgtatgata ggcatcttgc ctatcttttt tttgtaaaag
      721 taacaaggta ataatataat gcatagttta aaataagaag attagcccag gctaaatttc
      781 aagcatggag gtgctattat gaaaaacctt aacgatattc aagtgaaaaa aactgtcaaa
      841 gttgaagata tattatctag tggtaattta agagaaagaa tgttagcgct tggtcttact
      901 agaggagctg ttatagatgt agttagaaaa ggtccaaaaa ataatttaac tgtctataaa
      961 atcagaggta gcaagatagc tctacgacaa gaagaaagta gcttaatatt agtttctgac
     1021 atttaaaaat ttctagcagg aggatattaa tatggggttg acacataact caactaaaat
     1081 gagttcttta aaagacatgt ttgatataga caataaggaa gaccagtttg taattgcatt
     1141 ggctggcaat ccaaatacag gtaaaagtac tgtatttaat catcttacag ggcttagaca
     1201 gcatactgga aactggccag gaaaaactgt agctacagct cgtggtaatt ttaaatataa
     1261 aaataccgaa tatgcattaa tagatttacc aggaacatat tctttatttg ccttatcaca
     1321 agaagaaatt gtagctcgtg attttatatg ttttggtaat ccagatgcag taatagtagt
     1381 atgtgatgct acatgtcttg aaagaaattt aaatttagta tttcaagtga tggaacttac
     1441 agataaagtt attttgtgta ttaatttaat agatgaagct agaaaaaaag gcattactat
     1501 tgataaaaaa cttttagagg attctcttgg tattccagtt attttaactg ctgcaagaaa
     1561 tggctctggt atggatgaac tattggatac tttaaatgat gtatcttttg acaaatataa
     1621 attaaataat aaacctgtta gatacaatga aaatatagag aatgtggtaa aatcaattca
     1681 accagaattg gataatatta taccaggtat aaattctaga tggttgggac ttagacttat
     1741 tgatggcgat gaaagtatat ttgaatctat gagtaattat atagacaaag attctataga
     1801 tgctattaat gaagtaaaga aaaagattcc agacaatata aataaacaga aaataagaga
     1861 tgagtttact aaaatcaact atgattatgc taaaaaactg agtgatgagt gttgttctaa
     1921 tgttgctaaa aaatcaactg atagagaaga aaaagtagat aaaatattaa catctaaaat
     1981 atttgggctt ccaataatgc tcttattatt aggtactata ttgtggatta ctatagaagg
     2041 agctaactat ccatccactt tattatctaa tttattactc ggatttgagc caagtatttc
     2101 gggtatatta aatagtatta attgtccatc atggttaaat gatatgttgg ttttaggatt
     2161 atacagaaca ttagcatggg taatctctgt aatgctacct cctatggcaa tattttttcc
     2221 attatttacc ctacttgagg attttggata tttgccaaga gttgcattta acttagacca
     2281 cctatttaaa aaagcttgtg cacatggaaa acagtgtttg actatgtgta tgggatttgg
     2341 atgtaatgca gctggagtta ttggttgtag gataattgac tctccaaggg aaagacttat
     2401 agctatactt acaaacaact ttgttccttg taatggtcgt tttcctactt tgatagctat
     2461 atctacaata ttttttagct cagttataac caactctttc gtatcaagtg tagcaactgc
     2521 actttgcata actttactga taattctagg tgttataata acattgttgg tttcttatac
     2581 tctttctaaa acgctattaa aaggagttcc gtctacattt acattagaac ttcctcctta
     2641 tagagttcct caaataggaa gaactttata tacctctata atagatagaa caatatttgt
     2701 tttaggaagg gctgtaatgg tagccatacc tgctggtgtc ataacttgga tatttgcaaa
     2761 tatatatata ggagatttaa gtatattatc acatgttgca aactttttag accctttagc
     2821 taaattaatt ggtcttgatg gttttatatt acttgctttt attcttggat tcccagcaaa
     2881 tgagatagtt gtacctatac ttttaatggc ttacctagca actggttcta tgatagaatt
     2941 agatagcttt agtgctttag gtcaagttct tagagaacat ggatggactt atttgacagc
     3001 tttaaatgtt atgctgttca gtttactgca ttggccttgt gcaacaacac ttttaacaat
     3061 aaaaaaagaa actggtagtt taaaatggac tgccttagga ttcttaatgc ctactatact
     3121 tgcttttgtt gtttgttttt taacaactac agtttacaat ttatttatat aattagtatt
     3181 aactgacagt agacatatat tagagaaact atttaaaaat ttatatactt tattataaag
     3241 catagagata tattcaatga taagtaaaat tacttaacat ttgaatatat ctctatttta
     3301 attcttatct ggatacttgg attgaaattt cagtcatata attatcttca ttactttcac
     3361 aaaaaaagtc aagtatactc tcctcatacg aatttccaac aattttatat tcattttcat
     3421 atatatattt tttcaatttt tcgtaagatt tatatgaatc ctcatatttt ccaatatgtt
     3481 ttatgcaagc atacattcct tctggcttta catatagctt tttatccttt atttttttat
     3541 ctatcttcaa ataaaaactt gatatactta aatagtcccc attttttata ttttcattac
     3601 ttactattac tcccaactca tatccttgat acaaattttt agatttacaa taattggtga
     3661 aatctattaa ttccatcata aaatcactat tactcatatt actcaaatcg cttgatagaa
     3721 ctaaatattc ttcaaacttt ttggatatat aaatttcttc actattcatt ttagaaattc
     3781 cttcaactgt tatatttatt ctattttgca tcttagaact tatctttctt agttttttta
     3841 ttttttctct tatctttttt tcttcttctt taagcatttt cacacaaagc tggggagttc
     3901 tattttgtat atactctttt atttcctcta aactcattcc tatttctctc aatgattgta
     3961 atatatcaag tgtatcatat tgataaactg agtaatatct ataattattt ttatctttat
     4021 attcaggaga aaaaatacct attttgtcat agaaaatcaa tgtctgctta gatataccac
     4081 acaatttagc aaattcacct gttgtaaaat actgtttttc caagggttat acactccttt
     4141 tctttttgtt gtctatatag ttactatatt ctttattatt tactaaatca agtagttatt
     4201 attttagaaa atcaatatga ttttgatttt aaagcttaaa tatcagttag atgtcagtta
     4261 taaatatttt atccattccc atcataaaaa agatatcccc tcttaacttt tggatggaat
     4321 attatattta tacttgttag agtgaaaatt gtaataggtg ctacaataag atatttaatt
     4381 aaacataaag ttaattattg taataaaaaa tctttagata aattagagtt aaagtatgga
     4441 aatatagata gagaaaaagc tgttaaacta gaaatatttt attaatattt aatagaactt
     4501 gaatatatag aaatgagttt attaataaga agattagata ctgcaataac atcattagca
     4561 ttagtatcta ttataacaat agcatcatat tatttaatca gaaaaaatgt gtaattatgt
     4621 aagctattta taataaaaca ctgataaaat taataataaa aaggtaatag gatttctata
     4681 ctaccttttg gtttttataa tatagtgtaa ttttattata tttttgtatg ctaatataac
     4741 tagataatca taagataaca cattaaaaga aaattataaa aacttacatt ctgtatagtt
     4801 aatataaaac ataagtaata taaatttatg taaagcaaca aaagatggat ttacattcca
     4861 tatagttaat ataaaacgat ttcaaatgat tagaataaaa atatcagaat tgctatttat
     4921 attccatata gttaatataa aactaagaat ggaaaaggaa ataggagttc atattccaac
     4981 atttacattc catatagtta atataaaact atgtatcccc cattaaaaag gcttagatta
     5041 atttctattt acattccata tagttaatat aaaactcaaa catatctaag ttgtatttct
     5101 caccttgtag gtcatttaca ttccatatag ttaatataaa acaaggattt tgaatgtaat
     5161 ttatcataag ggtggcagat ttacattcca tatagttaat ataaaacatg aaagataatt
     5221 ggaaaactaa agtgcaatct aaaaaattta cattccatat agttaatata aaacggagaa
     5281 ttattaaaag gagttcccga agcagataga ataatttaca ttccatatag ttaatataaa
     5341 accattgtta ttattgtggt tctcccttag ctcataattt atttacattc catatagtta
     5401 atataaaaca tgatattatt ataatattat aaatataata atgaggattt acattccata
     5461 tagttaatat aaaactagat atttaaaata agcgaaaggg ggtcataaaa atatttacat
     5521 tccatatagt taatataaaa cattctaaag atattttgcc ttgcagttgg gatttcgaat
     5581 ttacattcca tatagttaat ataaaaccat cataggggct aatatagtta aaggtgttgg
     5641 cgtatttaca ttccatatag ttaatataaa acggtttggt tacaaggtat agtagaagga
     5701 tatagtggaa tttacattcc atatagttaa tataaaacat ggaactaaga gagtacacca
     5761 tcacatgata ctcaaattta cattccatat agttaatata aaactaaagc tggaaaagaa
     5821 acagctataa tgagtaataa taatttacat tccatatagt taatataaaa ccccaaaata
     5881 aacttagtat tttcaatact tacacgtata caactctctt aaatttgcag tgaacgagca
     5941 atagtgcaat tgataacgct tatcatacac tctcaacgcc ttatatttca actgttacgc
     6001 caaatttttt cacaaatatc gctcactgca aaattcctat aattttatta tatcataaat
     6061 atattacttt tgaatattga tactaattta taccataata aaagcaaaaa aacgtaatct
     6121 atctacatta gatttaaatt ctataactta aagtttttta cataatattt atgcttgttt
     6181 aatcaatcta atatatcatt cttgtatatt gcttttaata gtcttttttc ttaaataaat
     6241 atctatctga acaagcatag tttaattaaa aacttttgta ttcccttaaa actgtatcta
     6301 cctcttatta ttttatttat gatatattat ttttaatcta aaaatagata tctattttta
     6361 ttatagttat ccatttttaa aatactttaa tcatatacta ctcccaattt aacatccatt
     6421 gaaatgctat tttcttatat tacaatttat aattgatata tcttcaatat aattatttaa
     6481 tgtatttttg attttaaagc ttttcagtga tattattgat aaattagaat taattatcta
     6541 gctttaaaag gtcaaagatt tctgttgctg tctgcggttt tccaaataaa tatccttgta
     6601 tgtaatctgg ctttatttgt ttaactaaat catactcttc ttgtgtttct acaccttcta
     6661 aacatacttt tattcctacg tcatgacaaa ttgcaacaat aaaatgtata aatgtagcat
     6721 caaacttact ctttaatata tctttaacaa atactctatc aattttcact atatctattg
     6781 gagaaaattt taaaacttct agagacgagt atcccgtacc aaaatcatcc atagcaatat
     6841 aaattcctaa atcttgtaaa accttaaatt taaactgtaa aatactcata ttttgtactg
     6901 tatgactttc tgtcaattct aaaacaagat tttgataagg aaaattttca tcatctataa
     6961 tttttactat atcttcaata aatgtgtctt ctaatatttg aacagctgaa acgtttacac
     7021 tcactttaaa aaaaggatag taatcaatcc atttcctaaa agtacgtaat gccattcgaa
     7081 gcacccaagc ccctacaata tttatcatat cattttcttc taatattgga ataaattcca
     7141 aaggagaaat tgctttgcat ttgtcattag tccaccttaa aagaacttct acacctatta
     7201 ttttatgact ttctgtatct acttgtggct ggaatcgaag actaaacccc ctaaaatcat
     7261 cattaattga tgcctttaaa tcccgcatca tttctaatga atacatttta ttttttaata
     7321 tttcttggga gaaaaaaaca agcctgtttt taccatgctc tttcgcatat tgtaaagagt
     7381 aactagcaca cttataaagt tcttttactg tatctccatg ctttggatat attacacaac
     7441 cagcagaaat tgttatattt aatccatatt ttctccatag ttgaagatgt ataatcatat
     7501 tctgtatttg attatataga gttagaattt cattttcttc aacattatca acaagaatac
     7561 ccatttcgtc cccatcaagt ttataaagtt cagcattatc tggtaaaata gattgtatag
     7621 actgtgctag tgtttttatt atattatctc caaaatccct atcatacatc tcattaacat
     7681 ttttaaaatc gtcaatattt aataatacta tgcataattt ttcaatcata ggatttgaaa
     7741 ttttatcttc aaatacactc ataaattcat gataattcag taactgtgtc aatgaatcaa
     7801 tcttattttg tttacctaat tgcgtcatta ttcctgcaaa aatacttggt tctccaaatt
     7861 catcacgcat tagttgacct ctacatctta accatatata ttctccacta cgatttttag
     7921 ccctaaattc cactgtatga taatccatct ggtttttacc tatttcagtg tttgacttat
     7981 aaaaacgatt ccagtcttct ggatgtacaa tttttttcca gtaaacaagc ggattttcaa
     8041 caatttctcc tggtaagtca aataattcaa cttgtgatgg tgaatatcga aatacgcctg
     8101 ttttcatatt gcaaatgtat atatattcat ctgtacctct aatcaaagca tcataaagca
     8161 atgtagaatt ataatcaaaa ttgtctcttg aaaaattaaa aactcctttt tcttctttat
     8221 tatttgatat ttcatgaaat tcattaatct taattggttt atttaagctt gttgagtttg
     8281 caatatctat tgattgatgc atatgaacaa ctgtgccatc ttgccatgta attaaactat
     8341 cataattttc aaaaacacga tttaacttat tacatttttc ataccacttt attaatactc
     8401 cttttttatc attctttaat aattctaaaa ctttacaaaa actacaagta gaattttttt
     8461 caggatataa tacttgccaa caaacttttc cttctggatc taaaatatta tattcttctt
     8521 tcattttttt gttcataaaa ataatttcat tagtatgaat atttgtaaca tatatattaa
     8581 tctgtagttg atttaatata acttcaaaat tatgtttgtt catatactta tctcctccat
     8641 agttactgct ctttataaca acattgttaa aattattctt tttagaagat ttacttataa
     8701 cttatacata tacactaaat accttaattt aattagaaag tttattactt catattttaa
     8761 attataattg ataaaaaaaa atacagcaat atagtattac caatttaaaa aaaattaata
     8821 ttacataata catctctctc ttatattaaa ttttaacttg gtactaatct tgacaaattg
     8881 tccacaaaaa taaattatag attttattat tgttttaaat ttttataaaa aaatcagtat
     8941 tattgatact attgaagtta aaaagcatta atttctaaaa acatcataat ttttct
//
LOCUS       DAELLV010000150         8992 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.150, whole genome shotgun sequence.
ACCESSION   DAELLV010000150 DAELLV010000000
VERSION     DAELLV010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8992
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.150"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(101..943)
                     /locus_tag="KPD58_002630"
     CDS             complement(101..943)
                     /locus_tag="KPD58_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG0266383.1"
                     /translation="MIIQQIYNNNVVLVLDENIKKELILTGCGIGFQKKKGQEIDKSK
                     IERTFVIQDESFLDKISKLASQVDEKFFEVSTEIIAYAEENLNTKLYEYIYVALTDHI
                     AFAIKRYHENITIKNDLLHEIKRIHKKEYEIGKWAVDYINKEFDVKFPVDEAGFIAMH
                     IVNSNYKGSSKESLLITKIVKDILNIIRYYYRVEFKEDDINYDRLLTHLKFFAKRLVK
                     KEKINDTNNEIIDIIKVKYEKDYDCAYKIKTHVEKNYDYYVSQDELLYLTLHIKRVIS
                     VLNL"
     gene            complement(1204..3246)
                     /locus_tag="KPD58_002631"
     CDS             complement(1204..3246)
                     /locus_tag="KPD58_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HBG0266384.1"
                     /translation="MSILVTLLGLLVCTTISTVFSKKWSNIPLAIYQIVLGIILSILP
                     FKFSFSFNPEIFVICIIAPLLFSEGQNVSRKELLELRKPILLLAFGLVLITVFAGGIF
                     IHFLIPGMPLSVSFALAAVISPTDLVAVKSITQGLNFPKNMMSILEGESLLNDAAGVV
                     AFKVAVLATVTGVFSIEEAGIQFMITAFGGIIVGSILGYIIIKIRLSLHKWNLEEIPM
                     VIVIQIMTPLFVYFVAEEIGVSGILAVVMAGIAHGIEKEHLQNTTTKLRIISDNTWYV
                     LEYVLNGFVFTLLGFLLPSIYTGLSSKNENMALELTFISTLIVLILFVIRFIWVYLWH
                     NSFIKPKKNPLNNFFVGFLGFKDEEVVKESISKCKYSLIVATCGVHGTFTLATALSIP
                     FYLADKDVFPMRDTVLFISSEVILISLVLATVLLPRLLKNNVQESEPLLSDKEAYKLI
                     LKEAILKLSNEKAQEVQPVIHDLNEQLVDSEKGILNTPDNKLISMMIAEADQQGLLAV
                     LKLLEDGKISDRAFKLYRFYITKSRKSVQKSIFKIVKLKISMWIINRKVKKEVKEKIT
                     KIVSENKEIIKEFHHAYALSTKTAISFINKNTDEDNRHEALIAIRFYNRYLNWITRKI
                     QNDDKFEARVEYYRVMAIQSQRDSVQQLVETKQISREVAKELLENILYDEMMMIES"
     gene            complement(3398..4369)
                     /locus_tag="KPD58_002632"
     CDS             complement(3398..4369)
                     /locus_tag="KPD58_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HBG0266385.1"
                     /translation="MKKEESLLKVEGLKKYFEVKSGLFDKEIKCVKAVDGISFDLKKG
                     ETLAIVGESGCGKSTAGRTILRLIEKTEGKVEFNGVDIYKLEKEELRKLRPKMQMIFQ
                     DPYSSLNPRLNVEQIISEAVIEHNLVPKNEIKDNILNVVESCGLSKYHLNRKPGEFSG
                     GQRQRIGIARALALNPEFIVCDEPVSALDVSIQSQIINLLMDLQDKYKLSYLFISHDL
                     SVVNHIAHRVCVMYLGSIIEIATKGELYLHSQHPYTKALMSAVPIQDPTIKKNRIILK
                     GDIPSPTNPPSGCKFHTRCPYCKEICKKEIPVLKEISRNHFVACHLV"
     gene            complement(4370..5347)
                     /locus_tag="KPD58_002633"
     CDS             complement(4370..5347)
                     /locus_tag="KPD58_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0266386.1"
                     /translation="MSKALIEVKDLKVYFHTDKGIVKSVNEVSFNINEGETIGIVGES
                     GCGKSVTAMSLMKLLPTSKIEGGEIIFRGKDILKMNEDELMGIRGNEISMIFQEPMTS
                     LNPAFTVGSQIIEGIMIHQDLSKEEAKKKVIDMIKLVEIPRAEEIYNSYPHELSGGMR
                     QRIMIAMALSCNPKLLIADEPTTALDVTIQAQILDIMKNIKEKLNTSIMMITHDLGVV
                     AEMCDKVLVMYAGKIIEVAEVVELFKNPKHPYTIGLLKSKPALGKNKDKRLYSIPGQV
                     PNPIGMPDSCYFSDRCEKVCDKCRTQIPPLIELNSGHSIACWLYKKEEI"
     gene            5566..7125
                     /locus_tag="KPD58_002634"
     CDS             5566..7125
                     /locus_tag="KPD58_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0266387.1"
                     /translation="MKFKKLASLILVSSLMLTFTACASNDKDKSSGSKSGGTVVIPMA
                     SDPDIINGAFANVKEDSLASNIVYAPLYTYEKGNLVNYLAEKVDFKDSKELTIKLKSN
                     LKWHDGKPITAEDVLFTFNTVLDEKQNSPSRQYLLVGEKPVKVEKIDDLTVKITLPTA
                     SESFLYGISKISPIPKHVFEGESNIAKSEKNNNPVGSGAFKFKEWKKGESIVFEKNAD
                     YFGGEPKADSIALKIIPNEASQEAALNNGEISLMKTSAEGYEKAKSNSNLQTYTYSEE
                     RLNYIVFNQNISNMANKEVRQALSYALNRNEMIESAYGKEGSVPAKSILVPEADFYTE
                     EGVEGYDQDTNKAKDLLDKSGVKIDKLKIGYNTGRFGHKNYALVAQQELKKIGIEAEI
                     VPYESKAFFNILFSNSTECDMYVNGYAWGLEPNPYRGMFETGQYCNQTKYSNAEIDAL
                     WEKGFTELNKEKREEIYKQIQQDISKDAPIYTIDYEQNLMAAQKNLKGIKDAKPSPAI
                     LFEDWSKLYVE"
     gene            7231..8190
                     /locus_tag="KPD58_002635"
     CDS             7231..8190
                     /locus_tag="KPD58_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0266388.1"
                     /translation="MLKLILNRVKVLIPMLILISILSFGLLELAPGDPADAYINPLMS
                     SQDIENIRVNMGLDKPVYIRYLNWLKNTLSGNLGISYINHMPVTEQIMEKMGNTFILM
                     GTSLIFSILVAIPLGIFLAVNKNSITSKVSSVFNYIGVSIPSFWIGMILISIFSVKLN
                     IFPSGGMHTIGNDSIGDLVKHLVLPVITLGLYNTAIFTNYVEASVNEQLKKQYVITAR
                     AKGLSEKVILFKHVLKNSLTSLVTILGMSIQKLVTGAFVTEVVFSWPGMGRLMIDSIF
                     SRDYTVIMAITMLSALFLILGNLVADILYLLIDPKIKSSKGGF"
     gene            8193..>8992
                     /locus_tag="KPD58_002636"
     CDS             8193..>8992
                     /locus_tag="KPD58_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0266389.1"
                     /translation="MFTSILKKLKKDKSFAFACITLSIIIILIITSFILNIDSNAIDT
                     TSKLQEPSLKHIFGTDELGRDYFSRALYGGRISLSVGILSMLISVVFGTAVGVISGYI
                     GGKLDQFLMRLVDMLMSIPSFLVLIVLNTYLTPKVSTIILIIGFLSWMEVSRIVRGET
                     LKIKENEYCLAAKATGIKTRNILLRHIVLNLKETIVVAGSLNIANAILTESALSFLGL
                     GIQLPLASWGNMLQDAQRHIFDKLYLAVFPGLLIFLTVMSLTIISKKFS"
BASE COUNT         3110 a         1370 c         1124 g         3388 t
ORIGIN      
        1 taatgtttta aaattattat tttatattaa attttaaata atatattaaa attaaacata
       61 atatattaaa tctatacatt ctgtattaaa ctactcttta ttataaattc agtacagata
      121 taactctttt gatatgtaat gttaaataaa gtagttcatc ttgacttaca tagtaatcat
      181 aattcttttc tacatgagtt tttattttat atgcacaatc ataatccttt tcatacttta
      241 ctttaattat atctataatt tcattatttg tatcattgat tttttctttt tttactaatc
      301 gttttgcaaa gaattttaaa tgagtcagaa gcctatcata atttatatca tcttctttaa
      361 attcaactct ataataatat cttataatat ttagtatgtc ttttacaatt ttagtaatta
      421 atagtgattc ctttgaactt cccttatagt ttgaatttac tatatgcatt gctataaaac
      481 cagcttcatc tactggaaat ttcacatcaa attctttatt tatataatct acagcccatt
      541 tcccaatctc atattctttt ttatgtattc tttttatctc atgtagtaaa tcattcttta
      601 tagttatgtt ttcatgatat cttttaattg caaatgcaat atggtcagta agtgctacat
      661 aaatatattc ataaagctta gtattcaaat tctcctctgc ataagcaatt atttcagtac
      721 taacctcaaa aaatttctca tcaacctggc ttgctagttt actaatttta tctaaaaagc
      781 tttcatcttg tattacaaaa gttctttcaa ttttactttt atctatttct tgacctttct
      841 ttttttgaaa cccaattcca catcctgtta atattaattc tttttttata ttttcatcta
      901 aaactaatac aacattatta ttataaattt gttgaataat cataatcaat atctctccca
      961 aaatacaatt tcccttattc ataaaaaaat acctaaatta agatacaatt cacctgtata
     1021 ccttaaccta ggtattgcct gcttaccagt aacaatccag cttgttaacg cttatatatt
     1081 gtattcaatt tatggtttta ttctattata aataaatata tatgtcaagt aaattattga
     1141 aaactatttc ctatttttta gtattaattt taacatcaaa aatcaaattt tattccatta
     1201 tatttatgat tctatcatca tcatttcatc atagagaata ttttccaata actctttagc
     1261 cacttctctt gatatttgct tagtttcaac taattgttga acactgtctc tttgactttg
     1321 tatcgccatt actctgtagt attctactct tgcctcaaac ttatcatcat tttgaatctt
     1381 tctagtaatc caattcaaat aacgattata gaatctgatt gctattaagg cctcatgtct
     1441 gttatcttca tcagtatttt tatttataaa tgatatagca gttttagtag aaagtgcata
     1501 tgcatggtga aactctttga ttatttcttt attctcagat actatttttg taattttttc
     1561 ttttacttct ttctttactt ttctatttat aatccacatg cttattttta attttacaat
     1621 tttaaatata gatttttgaa cagattttct tgattttgta atataaaatc tatacaattt
     1681 aaaggctcta tcagaaattt taccatcttc taatagtttc aaaacagcta ataatccctg
     1741 ttggtctgct tctgcaatca tcatacttat aagtttatta tctggagtat ttagtattcc
     1801 tttttctgag tctaccaact gttcatttaa atcatgtata actggttgta cttcttgtgc
     1861 tttttcatta ctaagtttta aaatggcttc tttcaaaatt agtttatatg cctctttatc
     1921 agataataaa ggttctgact cttgtacgtt attttttaat aatcttggca gtaaaactgt
     1981 tgccaatact aaacttataa gaataacttc tgatgatata aataaaacag tatctctcat
     2041 tggaaataca tctttatcag ccaaataaaa aggtatagat aaggcagttg ctagtgtaaa
     2101 tgtaccatga acaccacaag tagcaactat tagtgagtat ttacatttag aaatactctc
     2161 ttttacaact tcttcatcct taaaacctaa aaaacctaca aaaaaattat ttaaaggatt
     2221 ctttttaggt tttataaaag aattatgcca taaatatacc catataaatc ttataacaaa
     2281 taaaataagt acaattaaag tagaaataaa agtcaattcc aatgccatat tttcattttt
     2341 actactaagt cctgtatata tacttggtaa caaaaaacca agcagtgtaa agacaaatcc
     2401 attcaataca tactccaaaa cataccaagt attgtctgat attatcctta atttagtagt
     2461 tgtattttgt aaatgctctt tttcaattcc atgagctatc cctgccatta caacagctag
     2521 gattccagat actcctattt cttctgctac aaaatataca aaaagtggag tcattatttg
     2581 tataactatt accattggaa tttcttccaa attccacttg tgtaaactaa gtcttatttt
     2641 tataattatg tatcctaaaa tacttcctac tattattcct ccaaaagctg taatcataaa
     2701 ttgaatccct gcctcttcta ttgaaaaaac tcctgtaact gtggcaagta ctgcaacctt
     2761 aaatgctact actccagcag catcatttaa taaagattca ccttcaagta tactcatcat
     2821 atttttagga aaatttaatc cttgtgtaat tgattttact gctactaaat cagttggaga
     2881 tattactgct gccaaagcaa atgaaacaga taatggcatc ccaggaataa gaaaatgtat
     2941 aaagattcca cctgcaaaca cggttattaa aactaaacca aatgcaagaa gcaaaattgg
     3001 ttttctgagc tctaatagct cttttctcga tacattttgt ccttcactga atagtaatgg
     3061 agctatgata catattacaa atatttctgg attaaatgaa aatgagaatt taaatggcaa
     3121 aatagacaat ataatcccta gtacaatttg atatattgca agaggaatat tactccactt
     3181 tttgctaaat acagttgaaa tcgttgtaca aaccaataat cccaacaatg ttactaaaat
     3241 tgacataact acctctcttt tcaataataa tattatagat taaacttata tatcaactaa
     3301 cttaatatta acagcatgaa atcccatctt aatcaaaaaa attttcagtt tattctatta
     3361 ttcttattat atacatcaaa tttaattttt gaaatatcta tactagatga catgcaacaa
     3421 aatgatttct tgatatttct tttaaaactg gaatctcttt tttgcatatt tccttacaat
     3481 aagggcatct tgtatgaaac ttacatccac ttggtggatt tgttggagat ggtatgtctc
     3541 cttttaatat aattctattt ttcttaattg tagggtcttg tattggtact gcgctcataa
     3601 gcgcttttgt atatggatgt tgagaatgta agtaaagttc tcctttagtt gcaatttcta
     3661 ttatagaacc tagatacata acacaaactc tatgtgcaat atgatttact acactcaaat
     3721 catgagatat aaataaatat gaaagtttat atttatcctg taaatccata agtaaattta
     3781 taatttgaga ttgaatagaa acatctagtg cagaaacagg ttcatcacaa actataaatt
     3841 ctggatttaa tgctaatgct cttgctatac ctattctctg tctttgacct ccagaaaact
     3901 cacctggttt tctatttaaa tggtacttag aaagaccaca actttctaca acatttaata
     3961 tattatcttt tatttcattc tttggcacta aattatgttc tataactgcc tcggatatta
     4021 tttgttccac atttaatctt ggatttaatg aactatatgg gtcttgaaat atcatttgca
     4081 ttttgggtct taactttcta agttcttcct tctctaactt atatatatct acaccattaa
     4141 attcaacttt accttcagtt ttttcaatta atctaagaat ggttcttcct gcagttgatt
     4201 ttccacatcc cgattctcca actatagcca aagtttctcc tttttttaaa tcaaaagaaa
     4261 ttccatctac tgcctttaca catttaattt ccttatcaaa taatccagat ttaacttcaa
     4321 agtacttttt aagaccttct acttttaata aactctcttc ttttttcaat tatatctcct
     4381 cctttttata taaccaacat gcaatactat gaccactatt caattctatt aaaggaggta
     4441 tctgagttct acatttatca catacttttt cacatctatc actaaaataa catgaatctg
     4501 gcatcccaat tggatttggt acttgtcctg gaatcgaata caatctttta tctttgtttt
     4561 ttccaagagc aggctttgat tttagaagtc caatagtata aggatgtttt ggatttttaa
     4621 ataattcaac aacctctgcc acttctataa ttttaccagc atacatcact aatactttgt
     4681 cacacatttc tgcaactact cccaaatcat gtgtaatcat cattatagat gtgtttaatt
     4741 tttcttttat atttttcatt atatctaaaa tctgagcttg aatagtaaca tctaaagctg
     4801 ttgttggttc atcagctata agtaattttg gattacatga taaagccata gctatcatta
     4861 ttcgttgtct cataccacct gataattcat gtggatatga attatatatt tcttcagctc
     4921 ttggtatttc taccagtttt atcatatcga ttaccttttt cttagcttct tcctttgata
     4981 aatcttgatg tatcataata ccttcaatga tttgacttcc aacagtaaac gctgggttta
     5041 aagatgtcat tggttcttga aaaatcattg atatctcatt ccctcttatt cccataagtt
     5101 catcttcatt catctttaaa atgtctttgc ctcgaaagat tatttctcca ccttctattt
     5161 ttgatgttgg aagtaacttc atcaaagaca tggctgttac agacttacca catcctgatt
     5221 ctccaactat tccaatagtt tcaccttcat ttatattaaa acttacttca tttacactct
     5281 ttacaatccc tttatctgta tgaaaataaa cttttaaatc tttaacctca attagtgctt
     5341 tactcattat tttctcctct cagcaaaatt acttaagata acatacacct tttgattgta
     5401 ttttataaaa atttaaatta caacattttc ttacatttta ttattttttt ctattaatta
     5461 taataatata atagttaatt tctatatata ttgatttaat aatgattact attatacaat
     5521 aaaaaatatg tgttaaatat tattttatgg aggttttata agaatatgaa atttaaaaaa
     5581 ttggcatcat taatcttagt ttcatcatta atgcttacat ttacagcctg tgcttccaat
     5641 gataaagaca agtctagtgg aagcaaatct ggtggaactg ttgtaatacc tatggcaagt
     5701 gacccagata taatcaatgg agcttttgca aacgttaaag aagactctct tgcatcaaat
     5761 atagtttatg ctcctcttta tacttatgaa aaaggaaatt tagtaaatta tttagcagaa
     5821 aaagtagatt ttaaagatag taaggaatta actataaaat taaaatccaa tttaaaatgg
     5881 catgatggaa agccaataac tgctgaagat gtcttattta catttaatac agttttagac
     5941 gaaaaacaaa attcaccatc aagacaatac ttattggtgg gtgagaaacc agttaaagta
     6001 gaaaaaattg atgatttaac agttaagatt actttaccaa ctgcaagtga atctttctta
     6061 tatggtatat ctaaaatttc tccaatacca aaacatgtgt ttgaaggaga atcaaatata
     6121 gctaagtcag aaaaaaataa taatccagtt ggttctggtg cattcaagtt taaggaatgg
     6181 aaaaaaggtg aaagcatagt atttgaaaag aatgcagatt attttggtgg agaacctaag
     6241 gcagattcta tagcgctaaa aataattcct aatgaagcat ctcaagaagc agctttaaat
     6301 aatggcgaaa tttcacttat gaagacatct gctgaaggat atgaaaaggc aaaatcaaac
     6361 tcaaacttac aaacttatac ttatagtgaa gaaagattaa actacattgt atttaaccaa
     6421 aatatatcaa acatggctaa caaagaagta agacaagcct tatcatatgc cttaaataga
     6481 aatgaaatga tagaatcagc atatggaaaa gaaggttctg tacctgctaa atctatatta
     6541 gttccagaag cagacttcta tacagaagaa ggtgttgaag gatatgacca agatacaaat
     6601 aaggctaaag atttattgga taaaagtggt gttaaaattg ataaattaaa aataggttat
     6661 aacacaggta gatttgggca taaaaactat gccttagtag ctcaacaaga gttgaaaaaa
     6721 attggtatag aagctgaaat agttccttat gaaagtaagg cattcttcaa tatactattt
     6781 agtaatagca ctgaatgtga tatgtatgta aatggttatg cttggggatt agaaccaaac
     6841 ccatatagag gtatgtttga aactggtcaa tattgcaacc aaactaaata ttctaatgct
     6901 gaaatagatg ctttatggga aaaaggattt actgagttaa acaaggaaaa acgtgaagaa
     6961 atatataagc aaatacaaca ggatatatct aaggatgcgc ctatttacac tatagattat
     7021 gaacaaaatt taatggcagc tcaaaaaaat ctaaaaggaa taaaagatgc taaaccatct
     7081 cctgcaatat tatttgaaga ttggtctaaa ctgtatgtag aataattgat gtaaaatttt
     7141 atattattaa ttagaaaagg agatgtctaa aaataatatt gttttattat tttaaaacga
     7201 gacatctctt ttaattagga gaatttactt atgttaaaat taatattgaa tagagtaaaa
     7261 gttttaatac ctatgttaat cttaatatct atattatcat ttggactttt agaattagct
     7321 cctggtgacc cagcagatgc atatataaat cctttaatgt cttcacagga tattgaaaat
     7381 ataagggtta atatggggct tgataaacca gtatatataa gatatttaaa ctggttgaaa
     7441 aacactttaa gtggaaactt aggtatttct tatataaacc atatgcccgt tacagaacaa
     7501 attatggaaa aaatgggaaa tacatttatt cttatgggta cttctttgat attttcaata
     7561 ttggtagcta tacctcttgg aatattttta gctgtaaata aaaattcaat aactagtaaa
     7621 gtatcatctg tttttaatta tataggtgtt tctatacctt ctttttggat aggtatgata
     7681 ctcatttcaa ttttttcagt taaacttaac attttcccat caggaggtat gcatactatt
     7741 ggaaatgata gtattggaga tttagtaaaa catttagttt tgcctgtaat aactttaggt
     7801 ctatataata ctgctatttt tacaaattat gtagaagcta gtgtaaatga acaattaaaa
     7861 aaacaatatg ttattacagc aagagcaaaa ggattatcag aaaaagtcat actatttaaa
     7921 catgtcttga aaaattcact gacatcattg gttacaatcc taggcatgag tatacaaaaa
     7981 ttagtcactg gtgcatttgt tacagaagta gttttctctt ggccaggtat gggaagatta
     8041 atgattgatt caattttttc aagagattat actgtaatta tggcaataac tatgttatca
     8101 gcactatttt taattttagg taatttagtt gctgatatac tatacttatt aattgaccct
     8161 aaaataaaat ctagcaaagg aggattctag ttgtgtttac aagtatctta aaaaaattaa
     8221 aaaaagataa atcctttgct tttgcatgta ttactcttag cataataatc atattaatta
     8281 taacttcttt tatattaaat atagactcta atgcaattga tactacttct aaacttcaag
     8341 aacctagctt aaagcatatc tttggtacag acgaacttgg tagagattac ttctctagag
     8401 ctctttatgg tggaaggatt tcacttagtg ttggtatttt atcaatgctt atttctgtag
     8461 tatttggtac tgctgtaggt gttattagtg gatatattgg aggtaagcta gaccaattct
     8521 tgatgagatt agtggatatg cttatgagta taccaagttt cttagttttg attgtattaa
     8581 atacttatct aacacctaaa gtttccacta ttatactaat tattggattc ttaagttgga
     8641 tggaagtttc aagaattgta cgtggcgaaa cattaaaaat aaaggaaaat gaatactgtc
     8701 tagctgctaa agctactggt atcaaaacta gaaatatact attaagacat atagttttaa
     8761 acttaaaaga gactattgta gttgctggtt cgttaaatat tgctaatgct atcttaactg
     8821 aatctgctct tagtttttta ggtcttggaa tccaattgcc tcttgcatca tggggtaata
     8881 tgcttcaaga tgctcaaaga catatttttg acaagttata tttagctgta tttccaggat
     8941 tacttatatt tttaacagtc atgagtttga ctataataag caaaaaattt tc
//
LOCUS       DAELLV010000151         8985 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.151, whole genome shotgun sequence.
ACCESSION   DAELLV010000151 DAELLV010000000
VERSION     DAELLV010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8985
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.151"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            133..1008
                     /gene="hslO"
                     /locus_tag="KPD58_002637"
     CDS             133..1008
                     /gene="hslO"
                     /locus_tag="KPD58_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBG0266390.1"
                     /translation="MRDYVLRATSGNGQVRAFVATTRNTVEEARRLHETTKVATAALG
                     RTLTATSIMGLMMKNDSDKLTVIIKGGGPIGTIIATSDSKGMVKGYVGNPQVEVEDYP
                     NGKLNVAAAVGTEGVVKVIKDLGLREPYNGTYPLVSGEIAEDFTYYFAVSEQTPSVVA
                     LGVLTKEDEVEFAGGFIVQLMPDAEEETIAKLEENVAKLPSITNMLKEGKSPEDILNI
                     VLDGLEPKILDTCEVGFICECSKERVKTALVAIGKKSLAQIIEEDKKAEVGCQFCNKK
                     YMYSEEELLEILKEM"
     gene            1399..2199
                     /locus_tag="KPD58_002638"
     CDS             1399..2199
                     /locus_tag="KPD58_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBG0266391.1"
                     /translation="MESKTKYIGISILILLMIFLIAKNFNGKLEIPIPESNSRKFKSA
                     AVFYPQHQDDEVLWGGSAIVDAIKQCGVDNVYVVLVSDGSGVNVFKANTKFRNLTRKQ
                     KEELRNNEFKSALRELGVKPQNVIILADIDKKEGTHYELMEKTILDFEHKFKGNVTHI
                     AHHYKYDDHIMHRKNGEVLKKLKREGKIKDDLYFVKPKYVKYIPRNERAIYKAMDFDD
                     YNKVKSACYEYKVVNSKRKMYGIGYISAHSYFEHLLNDPELTSVLSED"
     gene            2726..4966
                     /locus_tag="KPD58_002639"
     CDS             2726..4966
                     /locus_tag="KPD58_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG0266392.1"
                     /translation="MNNEFIILKGCKENNLNNISLKIPKRKITIFTGVSGSGKSSIVF
                     ETIAKESQRQLNERFSTFVRSFLPKYGEVKADCIENLSTPIIIDQSRLGGNSRSTLGT
                     ITDVNSFLRALYSRFGSEYIGNANMFSFNNIDGMCPHCHGLGKKLVPNMNQILDMNKS
                     LNEGAILLSGFGVGSWHWNIFAESGFFDINKRICDYSEVELEKFLHGKAEKIKIENAG
                     QTNMTYEGLMVKFNRLYLSREGEISEATKKKLSKLLIEDRCPICDGRRLNERVYRSLI
                     NGYNIADLTSMQIDELAEVIKSIDEPEAQPLIKGIIEKLNSIIDIGLGYLTLDRETSS
                     LSGGESQRIKMVKYLNSNLVDLMYIFDEPSVGLHPKDVYKLNNLLKKLRDKGNTIIVV
                     EHDPDVIKIADHIVDVGPKAGKYGGEVVYEGSYENLLTSGTLTGNALSKFLPIKESVR
                     EHSGYLEVKNCNKNNLKNVSIKIPKGVLTLVTGVAGAGKSTLIKDEFLKQNPSAVLID
                     QSPVSANSRSSLATYSGIMNNIRSIFSKTNGVNASLFSSNSEGACDNCKGSGIVEMNL
                     AFMESIKSTCNVCEGKKFKKEVLEYRFQGKNIIEVLEMSVLEAIEFFNLKQIKTKLQC
                     IEKMGIGYLTLGQTLDTLSGGECQRLKLASELNKESSVYILDEPTTGLHMADIENFIS
                     IIEEIVDNGNTVIIIEHNIDVIKRADWIIELGPEGGTKGGRVIFEGIPKQLCNNKISL
                     TAKYIV"
     gene            5380..6915
                     /locus_tag="KPD58_002640"
     CDS             5380..6915
                     /locus_tag="KPD58_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBG0266393.1"
                     /translation="MINDILTLLSERSDFFIKLSIEHLMISIISIIIAIVLGGIIGII
                     VSEFHNSSKPILGTINFLYTIPSISMLGFLIPFSGIGNTTAVIALTIYALLPMVRNTY
                     TGITNVDKNILEAARGMGSTDFQILYKIKLPLALPVIMSGIRNMVIMTIALAGIASFI
                     GAGGLGVAIYRGITTNNTIMTITGSILIALLALIFDFILGLVEKFFQRKRKKKNRKKV
                     FAILGSLILIATIFTSYLFNPSKTQTIHIATKPMTEQYILGEMLDILIEKNTGLNVEI
                     TQGVGGGTSNIQPAMENGEFDIYPEYTGTGWNMVLKKNELYTESMFSKLQSEYKTKLK
                     MQWTGMYGFSNSYALAVRKEVADKYNLKNITDLKDVSSKLTFGAEYDFYEREDGYTPL
                     CNTYGLNFKDTVDLDIGLKYQAINEGKIDVMPIFTTDGQYSVSDVVVLKDDKNFYPSY
                     QCGNVVRLEVLEENPELSKVFKMVGDILTDKEMAKMNYDVENNNLEPREVAREFLNKK
                     GLL"
     gene            6931..7692
                     /locus_tag="KPD58_002641"
     CDS             6931..7692
                     /locus_tag="KPD58_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0266394.1"
                     /translation="MTPIIQFKNIKKQYNDKTIIDNLNLDIEKGEFLTVIGSSGSGKT
                     TLLKMINGLILPDGGNILINQTDIKNEDLIKLRRRIGYCVQGSVLFPHMTVEENISYV
                     PNLLSKKNKLEVKSAVNKWMEIVGLPNDMKVRYPSELSGGQQQRVGIARALASSPEIL
                     LMDEPFGAVDEITRKQLQKEIKEIHKKTGVTIIFITHDIYEALILGTKTLVLNHGVVQ
                     QYDTPENILNTPANQFVDQLLNIRKSIIDDDNLKE"
     gene            8030..8899
                     /locus_tag="KPD58_002642"
     CDS             8030..8899
                     /locus_tag="KPD58_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG0266395.1"
                     /translation="MDNNLITIEKTLDYIEKHLLDEMLDLNVISKEIGYSKYHLHRMF
                     TSVVGFSVHNYIQRRRLTEAARMLIFTEKPIIEIALISGYDTQRSFSRGFKKMFKRSP
                     NFFRRHQDFLPLQLKFDIHNRKKLRGDMVLDVKKVNCADINIVGYSGKVRKGFHIIGK
                     CWRDLHKNKEMIENKVDLGFLIGVSDYSNFKEIENTPTFTYIAGAQVATFGKLPNGME
                     KFVLPKSNYIVFTFIGKNEDSMQNVVEYIYQEWFPNSSCQFNENNLYDFVKYGEKTDE
                     NGLSEIQFWVPII"
BASE COUNT         3449 a          925 c         1697 g         2914 t
ORIGIN      
        1 tagtaactat tacttagtaa aattaaaata aaatgggttt tgcccaaggt taagatagat
       61 aagtatttat agataaatta aggtagtaat ataataaaaa taagacaatt aacgggacat
      121 ggaggaatat taatgagaga ttatgtacta agagcgacat caggaaatgg tcaagttaga
      181 gcatttgtcg caactactag aaatacagta gaagaagcta gaagattaca tgaaactaca
      241 aaagtagcaa cagcagcact tggaagaaca ttaacagcca catctataat gggattaatg
      301 atgaaaaatg atagtgataa attgactgta ataataaaag gtggaggtcc aattggaact
      361 attatcgcca catcagattc taagggaatg gtaaaaggat atgtaggaaa tccacaagta
      421 gaggttgaag actatccaaa tggaaagtta aatgtagcag cagcagtagg tacagaaggt
      481 gttgtaaaag ttataaaaga tttaggtctt agagaaccat ataatgggac ttatccattg
      541 gtaagtggag aaattgcaga agactttact tattattttg ctgtatcaga gcagactcca
      601 tcagtagtag ctttaggtgt tcttacaaaa gaagatgaag tagagtttgc tggaggattt
      661 atagttcaat taatgccaga tgcagaagaa gagactattg ctaagttaga ggaaaatgta
      721 gctaaattac cttctataac taacatgtta aaagaaggaa aatcaccaga ggatatatta
      781 aatattgttt tggatggttt ggaacctaaa atacttgata cctgtgaagt tgggtttata
      841 tgtgaatgtt caaaagaaag agtaaaaact gctttggttg caattggtaa aaaatcattg
      901 gctcaaataa tagaagaaga taaaaaagca gaagttggtt gccagttctg taataagaag
      961 tatatgtata gtgaagaaga gttgcttgaa attttaaagg aaatgtagac tataaaaata
     1021 gttgtattga tataagtaat gttatagtga tatagtaaag tcgtattgat atagtaatta
     1081 aagtataatt tattaataat agacgtattt atattaaaaa ataagtttaa taaattttta
     1141 agcttaataa attttaagaa ttaagaagct ttctggctgt aatgtttgca atgttgcagt
     1201 tagaaggctt ttttatttta ttatagttcg tgtcaaagat tgtatgaaac aataaaatat
     1261 tgtaaaataa tttgtatatt tagacctgat tggcacaaag gtttataagg tatttgagtt
     1321 gattatgcga aattctatga agtagtaacc ctgtgacttt aatcatggga ggttcagaag
     1381 aaggagaaaa ttttaaatat ggaaagtaaa acgaagtaca taggaataag tatattaata
     1441 ttattgatga tatttttaat tgcaaagaat tttaacggaa aacttgaaat acctattcct
     1501 gagagtaata gtagaaaatt taaatcagca gcagtgtttt atcctcaaca tcaagatgat
     1561 gaggttttat ggggaggaag tgccattgta gatgctataa aacagtgtgg tgttgataat
     1621 gtatatgtgg tattagtatc agatggttct ggagttaacg ttttcaaagc taacacaaaa
     1681 tttagaaatt taactaggaa gcaaaaggaa gagcttagaa ataacgagtt taaatcagct
     1741 ttgagagagc ttggagtgaa acctcaaaat gtgattatac ttgcagatat agataaaaag
     1801 gaaggcacac attatgaatt aatggaaaag actatattgg attttgaaca taagtttaaa
     1861 ggcaatgtga cccatatagc ccatcactat aagtatgatg accatataat gcatagaaag
     1921 aatggggaag ttttaaagaa acttaaacgt gaaggtaaga taaaagatga cttgtatttt
     1981 gttaagccaa aatatgtaaa gtatatacca agaaatgaaa gagctatcta taaagcaatg
     2041 gattttgatg actacaataa ggttaagagt gcttgttatg aatataaggt tgtaaattca
     2101 aagcgtaaaa tgtatggtat aggatatatt tcggcacata gttattttga acatcttcta
     2161 aatgaccctg aacttacgtc tgtattaagt gaagattaat ttcttataag tagtaagtta
     2221 tgtttgataa gttgattgga attttctata gttttgaagg tattggaaat ttagttttat
     2281 ttaaagattt aaagatagag gtttttagtg tttaaaattt taaagttaag ttgaataatt
     2341 gtatagaatt atttgtgaaa gaagaaacag tgtaaaacat agtatcttat ttatggtttt
     2401 aaaatcatag gcaagatact ttttttatgt aactaatacc attttgagat tattaactta
     2461 agaattttat ataatttaac actcatatat aatgatgata tttaggtaga atagatgcaa
     2521 ttacgcttaa ataaaataac tgtatttttt ataaaaatca aaccctacac tatactacca
     2581 gataaaatct acatgctata atttgacctg tgtttataaa aaaaggagta atctaataaa
     2641 atagtatttc acttatgtat aactaacaac tattattacg attttctcta aataatttaa
     2701 agaaaaataa tgcaggaggt aaaatatgaa caatgaattt ataattttaa aaggttgcaa
     2761 agagaacaat ttgaacaata tttccttaaa aataccaaaa agaaaaataa ccatattcac
     2821 aggggtgtcg ggttctggaa agtcatctat tgtatttgag acaattgcaa aagaatcaca
     2881 acgccaacta aatgaaaggt ttagtacatt tgtaagaagt tttttaccta agtatggaga
     2941 ggttaaagct gattgtatag aaaatttgtc aacaccaatt atcatagacc aaagtagact
     3001 tggtggaaat tcaaggtcaa ccctaggtac tattacagat gtaaattcct ttttaagagc
     3061 tttatattca aggtttggta gtgagtatat aggtaatgct aatatgtttt cttttaataa
     3121 tattgatggt atgtgcccac actgtcatgg gcttggaaaa aagctagttc ctaatatgaa
     3181 ccaaattctt gatatgaata aatcattaaa tgaaggtgct atattgcttt cagggtttgg
     3241 agtgggttct tggcattgga atatattcgc tgaatcagga ttttttgata ttaataagag
     3301 gatatgtgat tattcagaag tagaattgga aaagtttttg catggtaaag cagaaaagat
     3361 taagatagaa aatgctggtc aaactaacat gacatatgag ggattaatgg ttaaatttaa
     3421 tcgcctatac ttaagtagag agggagaaat atctgaagct actaagaaaa aattgagtaa
     3481 acttcttata gaagatagat gtcctatatg tgatggaaga agacttaatg aaagagtata
     3541 taggtcttta attaatggtt ataatatagc agatttaact agtatgcaaa ttgatgaatt
     3601 ggctgaagtt attaaaagta tagatgagcc agaagcacaa cctttaatta aaggaataat
     3661 agaaaagtta aacagtatca tagatatagg tcttggatat ttaacactgg atagagagac
     3721 atcttctcta tcaggagggg aatctcagcg tataaagatg gttaagtatt taaatagtaa
     3781 tcttgttgat ttaatgtata tttttgatga accaagtgta ggacttcatc caaaagatgt
     3841 gtataaatta aataatcttt taaaaaaatt gcgtgataaa gggaatacaa ttatagttgt
     3901 tgaacatgac ccagatgtta ttaagattgc agaccatatt gtagatgtag gaccaaaggc
     3961 aggtaagtat ggtggagaag ttgtgtatga aggcagttat gaaaatctac ttacaagtgg
     4021 tactttgaca gggaatgctt taagtaaatt tcttcctatt aaagaaagtg taagagaaca
     4081 tagtggatat ttagaagtta aaaattgtaa taaaaacaat ttaaaaaatg tatcgattaa
     4141 gatacctaaa ggtgtattga ctctagttac tggagtagca ggagcaggta aaagtacgtt
     4201 gattaaggat gaatttttaa agcaaaatcc tagtgctgta cttatagacc aaagccctgt
     4261 ttcagcaaat tcaaggtcga gtttggcaac atatagtgga attatgaaca acataagaag
     4321 catattttca aaaacaaatg gcgtaaatgc ttctttattc agttcaaatt cagaaggagc
     4381 ttgtgataac tgtaagggtt ctggaatagt agaaatgaat cttgctttta tggagagtat
     4441 aaagagtacc tgcaatgttt gtgaaggtaa aaaatttaaa aaagaagttt tagaatatag
     4501 gtttcaaggg aagaatataa ttgaagttct tgaaatgtct gttttagagg caattgagtt
     4561 ttttaattta aaacagatta aaacgaaatt acaatgtatt gaaaaaatgg gtattggata
     4621 ccttacttta ggacaaactc ttgatacttt gtctggagga gagtgtcaaa gattaaagct
     4681 tgctagtgaa ttaaataagg aaagttcagt gtatatatta gatgaaccaa caacaggtct
     4741 tcatatggct gatattgaga attttataag tataattgaa gagattgtag ataatggaaa
     4801 tacggtcata ataattgaac acaatataga tgtaattaaa agagctgatt ggattattga
     4861 gctgggacct gaaggaggaa caaaaggcgg gagagtaata tttgaaggta ttccaaaaca
     4921 attatgtaac aataaaatat cattaacagc aaaatatata gtataataaa taaaatataa
     4981 taaaattata atgatatttt aataaatata atgggtatat aaaatgataa ttaaacttaa
     5041 cagtaataaa taatgtgaga aatattcaaa tttaattaaa taaagtgtca gaaatttgtt
     5101 taaattttag tattttaacg gttctgacct tttttcttat ttttatggag aatatcgagg
     5161 atggagtctc taatagttac ttttttgtgt ctacttgcaa gtttgattaa aaaatggcta
     5221 tacacacttg tgacttcagt catgggaggt tcagattata atcttaatat ataatttgtg
     5281 aaataatttg atgacgattt gctgatgtat aaacaataaa gattaactac attaaaattt
     5341 aaatcttgtt aagtaaaact tataaaagga gagagatgga tgataaatga tattctaacc
     5401 ttattatctg aaagaagtga tttttttata aagctatcca tagaacattt gatgatttct
     5461 attatttcta taataattgc gattgttttg ggtggtataa ttggtattat tgtaagtgaa
     5521 tttcataatt cttctaaacc tatattggga actatcaatt ttctatacac cattccatct
     5581 atttctatgc taggatttct gataccattt tccggaattg gaaatactac tgctgttatt
     5641 gcacttacca tttatgcatt attgccaatg gttagaaata catatactgg aattactaat
     5701 gtggataaaa atattcttga agcagctagg ggaatgggaa gtacagattt tcagatattg
     5761 tataaaataa aacttccgtt agccctacct gttattatgt ctggtattag aaatatggtg
     5821 attatgacaa ttgcattagc tggtatcgct tcatttatag gtgctggagg tctaggtgta
     5881 gccatatata gaggaatcac gacaaataac acaataatga caattactgg aagtatactt
     5941 attgctctat tagctttaat atttgatttt attttagggc tggtagaaaa attttttcaa
     6001 cgaaaaagaa aaaagaaaaa taggaaaaaa gtttttgcca ttctaggaag ccttattctt
     6061 atagcaacta tctttacgtc ttatttattt aatccttcaa agactcaaac tattcatatt
     6121 gctactaaac ctatgactga acaatatatt cttggtgaga tgttggatat tttaattgaa
     6181 aaaaacacag gtctaaacgt ggaaattaca caaggtgtag gagggggaac atctaatatc
     6241 cagccagcta tggagaatgg agaatttgac atttatccag agtatacagg tacaggctgg
     6301 aatatggtac ttaagaaaaa tgaattatat actgaaagta tgtttagtaa actacagtcg
     6361 gaatacaaaa ctaaacttaa aatgcaatgg actggtatgt atggtttttc taatagctat
     6421 gcattagcag ttagaaaaga agttgcagat aaatacaatt taaaaaatat aacggactta
     6481 aaagatgtat cttctaaatt gacatttgga gctgaatatg acttttatga gagagaggat
     6541 ggatatactc cattgtgtaa tacatatgga ctgaacttta aagatacagt tgatttagat
     6601 attggattaa aataccaagc tatcaatgaa ggtaagattg acgtaatgcc tatatttaca
     6661 actgatggtc agtatagtgt ttcagatgtt gtagttctaa aagatgataa aaatttctac
     6721 ccatcttatc aatgtggaaa tgttgttcgt ttggaagtat tagaggaaaa tccagaacta
     6781 tcaaaggtat ttaagatggt tggagatatt ttgactgata aagaaatggc taaaatgaat
     6841 tatgacgtag aaaacaacaa tttagaacct agagaagttg ctcgtgagtt tctgaataag
     6901 aaaggtcttt tataaattgg aggaaatata atgacaccta ttatacaatt taaaaatatt
     6961 aaaaaacaat ataatgataa aactataata gataatctta atttagatat agaaaaagga
     7021 gagtttttga ctgtaattgg ttcgtctggt tctggtaaga caacactctt aaaaatgatt
     7081 aatggtctga ttttgccaga tggaggaaat attttgataa accaaacaga tatcaaaaac
     7141 gaggacttaa ttaaattaag aagaagaata ggatattgtg tacagggaag tgttcttttt
     7201 cctcatatga ctgttgaaga aaatatttca tatgtaccaa atctcctaag taaaaagaat
     7261 aaattagaag taaaaagtgc tgtaaacaag tggatggaaa tagtgggatt gccaaatgat
     7321 atgaaagtgc gttacccatc agaattgtca ggtggtcagc aacaacgtgt agggattgca
     7381 agagctttag cttcttctcc agaaatttta ttaatggatg aaccttttgg tgcagttgat
     7441 gagattacac gaaagcaatt acaaaaagag ataaaagaaa ttcataaaaa aacaggagtt
     7501 acaattatct ttattacgca tgatatttat gaagctttga ttttgggaac aaagactttg
     7561 gtattaaatc atggagttgt tcaacagtat gatactcctg agaatatttt aaatactcct
     7621 gctaaccaat ttgtagacca gttattgaat ataagaaaat ctataataga tgatgataat
     7681 ttaaaagaat aaatttaaat ttaaaatcaa attgttgtga taaaaactat tgatttagag
     7741 ctaacttcaa gttgtatact tgaagtggaa aagttagatt aaggtataga agggcattta
     7801 gcagttggct gtagacgagg taactgctga aggtgctttc aataaatggc tagaacctgt
     7861 atctgttgaa gattataata aattgaaata agaagctcaa tatacagagt agataggcac
     7921 ttcaaataga agtgtctttt gtgtgccata aaataaatca aaatattagc aaagaatgtg
     7981 aaaaacaaaa taaaataata tgctatcttt aaaataggag ggatgctata tggataacaa
     8041 tttgattacc attgaaaaaa ctttagatta tatagaaaaa catcttttag atgagatgtt
     8101 agatttaaat gtaatttcta aagagatagg ctattcaaaa taccatttac atagaatgtt
     8161 tacatcagtt gtcggattta gtgtacataa ttatatacaa agacgtagac ttactgaagc
     8221 agcgagaatg ctaattttta cagaaaaacc aattattgag attgctttaa tctcggggta
     8281 tgatacacaa cgctcttttt caagagggtt taaaaaaatg tttaaacgta gtcctaattt
     8341 ttttagaaga catcaagatt ttttaccact acaattaaaa tttgatattc ataatagaaa
     8401 aaaattacgt ggagatatgg ttttagatgt taaaaaagtt aattgtgcag atataaatat
     8461 tgttggctat tctggtaaag taagaaaagg atttcatatt attggaaaat gctggaggga
     8521 tttgcataaa aataaagaga tgatagaaaa taaggtagat ttaggttttt taattggagt
     8581 tagtgattat agcaatttta aagagattga gaatactcct acatttacat acattgcagg
     8641 tgctcaagta gctacttttg gcaaattacc taatggaatg gaaaaattcg tattaccgaa
     8701 aagtaattat attgttttta cttttattgg gaaaaatgag gatagtatgc aaaatgttgt
     8761 agaatatatc tatcaagaat ggttccctaa ttcaagttgc cagtttaatg aaaataattt
     8821 atatgacttt gtaaaatatg gagaaaaaac tgatgagaat ggtttgagtg aaattcagtt
     8881 ttgggttcca atcatttaaa agaaggtatt atgtataaag tagatggatt tatataggca
     8941 aagttttaca gcagtatgta tttataaata atttatagtt tgtag
//
LOCUS       DAELLV010000152         8965 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.152, whole genome shotgun sequence.
ACCESSION   DAELLV010000152 DAELLV010000000
VERSION     DAELLV010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8965
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.152"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            217..930
                     /locus_tag="KPD58_002643"
     CDS             217..930
                     /locus_tag="KPD58_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2140 domain-containing protein"
                     /protein_id="HBG0266396.1"
                     /translation="MGKVAKILLSLIVIVLVLVGIVVYILTPKERYDVSSQNNKIIDE
                     EYLSKGSYINSMEVVKNPLRMVGKISVSEEEFKNLIYTLMQKHGINELENNFVEMKNG
                     KIKVAGPYKVFGLVNSQYELELRPTLKDGNLVVSLENVKIGKFKLSDKMLEKILSNYN
                     KKVPFEVNGNKITLEKSYLYPITLKNISIKKGNVDLDMEVEIDLKSQINGALGYLKDS
                     GKVQDILNHLINLKKTSVG"
     gene            1089..1652
                     /locus_tag="KPD58_002644"
     CDS             1089..1652
                     /locus_tag="KPD58_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266397.1"
                     /translation="MNKVYIVTTYTGTILSYLIRNISKKLYTHVSISLNENLKPMYSF
                     GRLNPRNPFIGGFVEENINQGLYAIRKNTVCRVYSLEVDNLQYENLYKNIKLISDYRE
                     DYYYDTMALIYLLMNIQRQSEYKYVCSNFVADMLEKSEIDILNKQAFEVTPNDFYNLS
                     GLTLEYEGLLSEYNSRQHSYVSKIANI"
     gene            2049..3749
                     /locus_tag="KPD58_002645"
     CDS             2049..3749
                     /locus_tag="KPD58_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266398.1"
                     /translation="MKRYLKTYGVALIIVIIYAFIKLPVLRLDFTSLFSVGIIFFVVA
                     GILDMMLDRNERASKMAKYNFSIAIALLVYIVVVPFITSTPVLHAKSYRELLGKVTES
                     KFTDDISPVSVNDIRLVDEDMAMKLGDKKLGEVPAIGSVSKLGKFHIQNVDGELYWVA
                     PLVHRDIIKWITSLSGTSGYVKVSASNPQDVQLVQEIDGKPIKIVYQPEAYLHQDLQR
                     HLYIHGIVNVGMTDFTLEINDEGRPYWVVSLYEHKIGYGGANATGIATVDAETGKINV
                     YDVKNTPKWVDRIQPQSFVTDQIKDWGVYVNGFLNSVISEKGVLVPTEGTSLVYGNDN
                     RSYWYTGITSSGGDESTIGFMLVDSRTKEARLYKQPGATETAAMKSAEGNVQEKNYEA
                     TFPVMYNILGQPTYVSSLKDKAGLVKRVAFVSVEDYNVLGIGEDKNEALRNYKDALES
                     KGNDLKLDNDLKDEVLEGTVTRISPDVRGGNTNYYVTLDSNKDIIFRATSKVSSELPL
                     TQVGDKVKISYSKEESGVIEMSEFDNLNIANFEDKKAKEKTGTDENTDSKDSENQGNV
                     "
     gene            3919..4536
                     /locus_tag="KPD58_002646"
     CDS             3919..4536
                     /locus_tag="KPD58_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266399.1"
                     /translation="MTNMEKEILLLISRVRCVTESQFRKIYGDQKRYNKKNFKKTLRK
                     MCSEYTLKKYPCDVDYYNYKDLSYVYYLNGSKLFKGDDLIKALVGSELVVRINTAGCD
                     VKRFYRNVSVDDVKYDLFIEYTDQFGNIKQVLVDVDLNNEIDISKYENLSKKIDKSTI
                     PFFKVPNVLVVTPDNLSELNDLERYDNEYSINFIDLGLNKLFNCI"
     gene            4897..6090
                     /locus_tag="KPD58_002647"
     CDS             4897..6090
                     /locus_tag="KPD58_002647"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBG0266400.1"
                     /translation="MSIIVQKYGGSSVADTEKIKSIAENIIERRKENPQMVIVVSAMG
                     KSTDEYITLAKELSNEPSKRELDALMSTGEMISASLLSIALNALGCKAISYNAYQLNI
                     KTSGLHGKSQIDDINVNRIKNSLDEGNVVIVTGFQGINEDGDVTTLGRGGSDTSAVAL
                     AVKLNGKCEIYTDVDGIYFTDPRKYSKASKLDEIEYEEMLELASLGAQVMHSRSIELA
                     QKYGVEIYVGRTCGTEKGTYIRGGKDMKLEDKVITGLATSDDDSSITIKDFKAENISS
                     LFEDIATIGISVDMISQTAPILDKISVSFTVPKEELGECKKIVSKYTDEEHVVIDNNI
                     TKFSLVGLGMKNTSGVAAKVFKIFNENGIMIKLITTSEIRITCAINSDDKQVAIEKIA
                     EVFNI"
     gene            6108..7400
                     /gene="lysA"
                     /locus_tag="KPD58_002648"
     CDS             6108..7400
                     /gene="lysA"
                     /locus_tag="KPD58_002648"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBG0266401.1"
                     /translation="MKLHGTMTVNNDELYIGGVSCLELSKKYQTPLYVFDEALVRQNC
                     REYVEYFKAREGKNKVAYAGKAFLPLYMCNLINEEQMCLDVVSGGELYTAYKSNFPME
                     KVFFHGNNKTKEEINMGLDLGVGKFVVDNFYELDLLEALCEDKGKTQEIYFRITPGIE
                     AHTHDYIKTGQIDSKFGFALVNGDLFRAIEKLSEYKNIKLVGLHAHIGSQIFDVEPYI
                     DTVDIMMNLVKEIKEKFNIQLTEIDLGGGVGVYYSEGDKPKTIKEFCETIITKAETSC
                     KELGIEVPTLVIEPGRSIVANAGSTLYTVGSIKDIKDVRVYVSVDGGMTDNVRPSLYQ
                     ASYECSIVNKINHEAMNHVTIAGKCCESGDILIGDINIMDIKSGDILITTSTGAYGYS
                     MSSNYNKIPRAAVVSVFDGNDKIICKRQSYEDLLALEI"
     gene            7617..8276
                     /locus_tag="KPD58_002649"
     CDS             7617..8276
                     /locus_tag="KPD58_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG0266402.1"
                     /translation="MRGRVILLISILSIFLVGCSFNKKDEINLVEQGKTYLEKHNYKK
                     AMESLSSALEEDSTNENARAMYMQAMRMSNMTEFEELKNYEGAIKELKLIEKIKNGSS
                     VIKEEATKKKEELTKLEKEQNEAEEQRKKKAKDTAGEDRYRVESDARKSSYSGSSKNN
                     KSSSKNNDKKSDSNKSDNNNSSSQNQEKPNNSNPTPAPTPQDTTGGSDSGGNSQNSNN
                     Q"
     gene            complement(8431..>8965)
                     /locus_tag="KPD58_002650"
     CDS             complement(8431..>8965)
                     /locus_tag="KPD58_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG0266403.1"
                     /translation="DKEKIALGFKRFLYSSTARNKADNIIEFFRTTHDIFYDYLLGRI
                     LDSLIIGIIAFLGFQFVIRIENALFLASIIFLGNIIPYFGPFIGAIPPIAMTILYSPQ
                     KTIWVIAFLFILQQLDGNFIEPKVMGNQVGIGAIWVISAILVGQSLFGFIGVFLSVPI
                     AAVVKTYVDKYIDNRLQ"
BASE COUNT         3625 a          919 c         1577 g         2844 t
ORIGIN      
        1 gattagaaaa aaataaaggt gtaattaaga gtattatgca aaatattaac ttttatacta
       61 aaaacatgtt tttacagttt atttacaaaa cattagattg gttgaattaa tttttaaaag
      121 actgtattat agtatgtata ttgttaagaa tattatgtga agttagttat ctaaatttat
      181 tttaaatctg atgacagatt tgtaaaggag acgattatgg gaaaggttgc aaagattttg
      241 ttaagtttaa ttgttattgt tttagtactt gtaggaattg ttgtatatat cttgacacca
      301 aaagaaagat atgatgttag ttcacaaaat aataaaatca tagatgaaga atatttatca
      361 aaaggctcat atataaattc tatggaggta gtaaaaaatc cacttagaat ggttggaaaa
      421 atatctgttt cagaggaaga gtttaaaaat ttaatatata cattgatgca gaagcatgga
      481 attaatgaac ttgaaaataa ttttgttgaa atgaaaaatg gtaaaataaa ggtagcagga
      541 ccatataagg tatttggttt ggtaaatagt caatatgaac ttgaattgag acctacttta
      601 aaggatggaa acttagttgt aagtcttgag aatgtgaaaa ttggaaaatt taaactaagt
      661 gataaaatgc tagaaaaaat attaagtaac tacaataaaa aagttccatt tgaggttaat
      721 ggaaataaga ttactttgga aaaaagttat ctttatccaa taacacttaa aaatatatct
      781 ataaaaaaag gaaatgttga tttggatatg gaagtagaaa tagatttaaa atcacaaata
      841 aatggggcat taggatattt aaaggactct ggaaaggttc aagatatact aaatcatctg
      901 attaatttga agaaaacatc tgttggttaa aaatatagaa aacttatatt tatattttta
      961 ttatattata taatattaaa gtaccttaaa atcaaaattt gtaaaaccac atttattatg
     1021 ttagaatatc ttatgatatt aaattgtaag tgtataatat tatttagtta ctagattggg
     1081 gggaaatgat gaataaagtc tatattgtaa caacatatac aggaactata ttatcttact
     1141 taataaggaa tataagtaaa aagctgtata cacatgtgtc aatatcatta aatgaaaatc
     1201 taaaaccaat gtattctttt ggaaggctaa atccaaggaa tccatttatt ggaggatttg
     1261 ttgaagaaaa tataaatcaa ggattatatg ctataagaaa aaacactgtg tgtagggtat
     1321 attcgttaga agtagataat ttacaatatg aaaatctata taaaaatata aaattaatta
     1381 gtgattatag agaagattac tattacgata caatggcatt aatatattta ctaatgaata
     1441 ttcagagaca atctgaatat aaatatgtat gttcaaattt tgttgctgac atgttagaaa
     1501 aaagtgagat agacatttta aataaacaag catttgaagt aacgccaaat gatttttata
     1561 atttatctgg cttaacctta gagtatgaag gtttattatc agaatataat tctagacaac
     1621 attcttatgt atcaaaaatc gcaaacattt aaaaacttga tttagagttt ttcatataaa
     1681 aaaatattgc tagataatta ggctgaagat agtagtttaa cttcgatagg attaagaatt
     1741 tgtaaatctt agttagaata ctttcttcag tttttaaatt tctgctaata tttgtatttt
     1801 tataatattt ttggatatat ataatacaga atagtgcaaa aatcccactc ctttaggtgc
     1861 gggatggata gtacaaacag tgtataaaaa gtatttatac atacacgtat aatgttaaat
     1921 atagttgaat aagcagaact ataaatatat aaagatatct taatatataa taaatatgat
     1981 aaatatttat tttttgtaaa tatatggata taatagtaat aataacattt tttagagagg
     2041 agtattaaat gaaacgatat cttaaaacat atggagttgc attaataatt gtaataattt
     2101 atgcatttat taaattacct gtactaagat tagattttac atctttattt tcagtaggaa
     2161 taattttctt tgttgtagca ggaatactag atatgatgtt agatagaaat gaaagagcat
     2221 ctaaaatggc taaatataat ttttcaatag caatagcttt gcttgtgtat attgtagtag
     2281 ttccctttat aacttctaca cctgtacttc atgcaaaatc atatagagaa ctattgggaa
     2341 aagtaactga aagtaaattt actgatgaca ttagccctgt cagtgtaaat gacattcgtc
     2401 ttgtagatga agatatggca atgaaattag gtgacaaaaa gcttggagaa gttcctgcaa
     2461 tagggagtgt atccaaatta ggaaaatttc atatacaaaa tgtagatgga gagttgtact
     2521 gggtagcgcc tcttgttcac agagacataa ttaaatggat aacatctcta agtggtacaa
     2581 gtggttatgt aaaagtatca gctagtaatc ctcaagacgt ccaattagtt caagaaatag
     2641 atggaaagcc aataaagata gtttaccaac cagaagccta cttacatcag gatttacaaa
     2701 gacatttata catacatggt attgtaaatg ttggtatgac agatttcact cttgaaataa
     2761 atgatgaagg aagaccttat tgggttgtaa gtttatatga acataaaatt ggctatggag
     2821 gagcaaatgc aacaggaata gccactgttg atgcagaaac tggaaaaata aatgtttatg
     2881 atgttaaaaa cacgcctaaa tgggtggata gaatacaacc acaaagcttt gtaacagacc
     2941 agattaaaga ctggggtgtt tatgttaatg gatttttaaa ctctgttata tcagaaaagg
     3001 gagttttagt accaacagaa ggtacatcat tagtttatgg caatgataac aggtcttatt
     3061 ggtatacagg tataacatct tcaggtggag atgaatctac aattggattt atgttagttg
     3121 attctagaac aaaagaagct agattatata aacaaccagg agctactgaa acggctgcaa
     3181 tgaaatcagc tgaaggtaat gttcaagaaa aaaattatga agcgacgttc cctgtaatgt
     3241 ataatatatt gggtcagcca acatatgtat catcattaaa agataaggct ggtcttgtta
     3301 agagagttgc atttgtatct gttgaagatt ataatgtact tggaataggt gaagataaga
     3361 atgaagcatt aagaaactat aaggatgcat tagaatctaa gggtaatgat ttaaaacttg
     3421 ataatgattt aaaagatgag gtattagaag gcacagtgac aagaataagc ccagatgtgc
     3481 gaggtggtaa cactaattat tatgtaactt tagattcaaa taaagatata atatttaggg
     3541 ctacttcaaa agtatctagc gaattgccat tgactcaggt tggtgataaa gtcaaaatat
     3601 cgtattcaaa agaagaatct ggtgtaatag aaatgtcaga atttgataac ctaaatattg
     3661 caaactttga agataaaaaa gcaaaagaaa aaacaggaac tgatgaaaat acagattcca
     3721 aagattctga aaatcaaggt aatgtataat ttaaataaaa agattgataa tagtatttaa
     3781 catagtgata gctaattttt atagaattta attctataaa aattagctat tttattttaa
     3841 ttctttgtaa atttaatgtc ataaaattaa tataatatta gtacaacttt gcaaataata
     3901 cttcaaggag aatgtactat gacgaatatg gaaaaagaga tattactttt aattagcagg
     3961 gttagatgtg ttacagagtc tcaatttaga aaaatttatg gagaccaaaa aagatacaat
     4021 aaaaaaaatt ttaaaaaaac tttgagaaaa atgtgtagtg aatatacact aaaaaaatat
     4081 ccttgtgatg tggattatta taattataaa gacctctctt atgtatacta tttaaacggt
     4141 agtaaacttt ttaagggaga tgatttaata aaggctctag taggatcaga attagttgtt
     4201 agaatcaata ctgcaggttg tgatgtaaaa agattttacc gaaatgttag tgtagatgat
     4261 gtgaaatacg atttatttat tgaatataca gaccaatttg gtaatataaa gcaagtttta
     4321 gttgatgttg acttaaataa tgaaatagac atttctaaat acgaaaatct ttctaaaaaa
     4381 atagataaat ctacaattcc attttttaaa gtaccaaacg ttttggtagt tacaccagat
     4441 aatctatccg aattaaatga tttagagaga tatgataatg agtatagcat aaattttata
     4501 gatttaggat taaataaact atttaattgt atataagcta actttagaaa agaaaactat
     4561 cgatttgcca cattttgtaa aataattgtt gcaatattat atagaatatg ataaaactat
     4621 attaataaat taattaaaaa taatgagcct agggtagagg tagctgtatt caatagtatt
     4681 caataggagc tggtaagcgt tgataattga tgaaagggta tatagccgaa gaaattttgt
     4741 tgacggacaa attttctgga cttatgcaga atatgcataa ggctgtcact tttaaagtgg
     4801 tgagctacaa ggttacttgg tctatgaaat ttacacgtcc aaagtatacc tttgggcgtt
     4861 tttatttatc aaaatttagc ttgaggggga gaaagcatga gtataattgt tcaaaaatat
     4921 ggtggaagtt cagtagcaga tactgaaaaa ataaaatcaa tagcagaaaa tataatagaa
     4981 cgaagaaaag aaaatccaca aatggtaata gttgtgtctg ccatgggaaa atccactgat
     5041 gaatatataa ctttagcaaa ggaattgagt aatgaaccta gtaagaggga attagatgct
     5101 ttaatgtcta ctggtgaaat gatttcagca tctttattat caatagcatt aaatgcactt
     5161 ggatgtaaag ctataagtta taatgcttac cagttaaata taaaaacgag tggtcttcat
     5221 ggaaagtctc aaattgatga tataaacgta aacagaatta aaaatagttt agatgaagga
     5281 aatgtcgtga tagtaactgg atttcaggga atcaacgaag atggtgatgt aaccactctt
     5341 ggaagaggtg gttcagatac atctgctgta gctcttgcgg ttaaattaaa tggaaaatgt
     5401 gagatatata cagatgttga tggaatttat tttacagacc caagaaaata ttcaaaggct
     5461 agtaaacttg atgagattga atatgaagaa atgcttgagt tagcgagttt aggagcacaa
     5521 gtaatgcatt caagaagtat agagcttgca caaaaatatg gtgttgaaat ctatgtcgga
     5581 cgtacatgtg gtacagaaaa aggaacatat ataagagggg gaaaagacat gaaattagag
     5641 gataaagtaa taacaggatt agctactagt gatgatgaca gttctataac aataaaagat
     5701 tttaaagctg aaaatatatc tagcttattt gaagatatag caactatagg tataagtgta
     5761 gatatgataa gccaaactgc acctatttta gataagataa gtgtatcatt tacagttcca
     5821 aaagaagagt tgggagaatg taaaaagata gtatctaaat atacagatga agaacatgta
     5881 gtgattgata ataatatcac taaattttct ttagtaggtc ttggtatgaa aaatacttct
     5941 ggagttgctg ctaaagtatt taagatattc aatgaaaatg gtataatgat aaaacttata
     6001 actacatcag agattcgtat aacttgtgct ataaattctg atgataagca agtagctata
     6061 gaaaaaatag cagaagtatt taatatataa aatttgggga ggctattatg aaacttcatg
     6121 gaactatgac tgtaaataat gatgaattat atattggagg agtgagttgt ttagaactta
     6181 gtaaaaaata tcaaactcca ctttatgtat ttgatgaagc tttagttaga caaaattgta
     6241 gagaatatgt agaatatttt aaggctagag agggaaaaaa taaggttgct tatgcaggga
     6301 aagctttttt acctctttac atgtgtaatc ttataaatga agaacaaatg tgtttagatg
     6361 tagtttcagg aggcgagtta tatacagctt ataaatcaaa ttttcctatg gaaaaagttt
     6421 tctttcatgg aaacaataag actaaagaag aaataaacat gggattagat ttaggtgtag
     6481 gtaaatttgt ggtggataat ttttatgaat tagatttgct tgaagcatta tgtgaagata
     6541 aaggtaaaac tcaagagata tactttagaa taactcctgg tatagaagct catacacatg
     6601 attatataaa aactggacag atagattcta aatttggatt tgctttagta aatggagatt
     6661 tatttagagc aatagaaaaa ttaagtgaat ataaaaatat taaacttgtg ggtcttcacg
     6721 ctcatatagg ttcacaaata tttgatgtag aaccatacat agatacagtg gatataatga
     6781 tgaacttggt aaaagaaata aaagaaaaat tcaacataca actaacagaa attgacctag
     6841 gtggaggagt tggcgtttat tatagcgaag gagataaacc taaaactata aaagagtttt
     6901 gtgaaactat aatcactaaa gcagaaactt catgtaaaga gcttggaata gaagtaccca
     6961 cattggtaat agaaccagga agatcaatag ttgcaaatgc aggaagtaca ttgtatacag
     7021 taggttctat aaaagatata aaagatgtta gggtttatgt aagtgtagat ggaggtatga
     7081 cagataatgt aagaccttcg ttgtaccaag ctagttatga gtgtagtatt gtgaataaaa
     7141 ttaatcatga ggctatgaat catgtaacaa tagctggaaa atgttgtgag agtggagata
     7201 tattaattgg agatataaac ataatggata ttaaaagtgg agatatactt ataacaacat
     7261 caactggtgc atatggatat tcaatgtctt caaattataa taaaatacca agagcagcag
     7321 tagtatcagt atttgatgga aatgataaga taatttgtaa gagacaaagc tatgaggact
     7381 tattagcttt agagatataa aatggagaag tatataataa aaattaaata ttaaagttta
     7441 ttatgagcag atttgctagg atatgtatct ctaaattaat ttttggttta ttgtatcgtt
     7501 cgtagcatat ctgcttattt tttgttttac taacaaattt cttgtaataa atataaaata
     7561 ttttaaaaac tttataatta tgataaaatt taaagtatat gacaaggagg gatttaatga
     7621 gaggaagagt aattttatta atatcgatat tatctatatt tcttgtagga tgttcattta
     7681 ataaaaaaga tgaaataaat ttagtagagc aaggaaaaac atatttagaa aaacataact
     7741 ataaaaaagc tatggaatca ttatcctctg cactggaaga agatagtact aatgagaatg
     7801 caagagctat gtatatgcaa gctatgagaa tgtcaaatat gacggagttt gaagaattaa
     7861 aaaattatga gggtgcaatc aaggaactta aacttataga gaaaatcaaa aatggttcct
     7921 ctgttattaa agaagaggca actaaaaaga aagaagagtt aactaaatta gaaaaagaac
     7981 aaaacgaagc agaggaacaa agaaagaaaa aggcaaaaga tacggctgga gaagataggt
     8041 atagagttga atcagatgca aggaaatctt cttatagtgg aagtagtaaa aataataaat
     8101 ctagttctaa aaataatgat aaaaaatcag atagtaataa gtctgacaat aataacagtt
     8161 cttctcaaaa tcaagaaaag ccaaataata gtaatccaac accagcacca actccacaag
     8221 atactactgg tggttcagat agtggtggaa attctcaaaa ctcaaataat caataagtat
     8281 aaaatatatg cataaattta atgaacaaat tatatattat aaaaaatatc tcttagcaat
     8341 tttttcttac taagagatat tttttgagaa ataatttaat aaataacagt taaattttaa
     8401 actaaaaatt aaagtcacag tagagataag ctattgtaaa cgattatcta tatatttatc
     8461 aacataagtt tttacaacag cagctattgg tactgaaaga aaaactccta tgaaaccaaa
     8521 tagagattgt cccaccaaaa tagcagatat tacccatata gctcctatac caacttgatt
     8581 acccataact tttggttcta taaaatttcc atctaattgt tgcaatataa atagaaatgc
     8641 aatgacccat atagtttttt gtggactata taatatagtc atggctattg gagggattgc
     8701 accaataaaa ggaccaaaat agggaattat attacctaag aatattattg aagctaaaaa
     8761 taaagcattt tctattctga taacaaattg aaatcctaaa aatgcaatta taccaataat
     8821 aagagagtct aatattctac caagtaaata atcatagaaa atatcatgag tagtcctaaa
     8881 aaattctata atattatctg ccttgtttct agcagtagaa gaatatagaa atctcttaaa
     8941 tcccaatgca attttttctt tatca
//
LOCUS       DAELLV010000153         8903 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.153, whole genome shotgun sequence.
ACCESSION   DAELLV010000153 DAELLV010000000
VERSION     DAELLV010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8903
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.153"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(326..1222)
                     /locus_tag="KPD58_002651"
     CDS             complement(326..1222)
                     /locus_tag="KPD58_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG0266404.1"
                     /translation="MKDKNTYFNRKGQENFIDIFLKDDIKKGANFKRHWHEHLQIYYF
                     IDGIAYLECGLDNFKVVPGNIVIANSNELHYLESLSDNLKFYSIRIEPTFLFSNQVDL
                     LQTKYLAPLALNHIVFNNLIQNDSLILKCITTILDEYHNKEVGYELSIKANIYQLIVL
                     LLRSHIDKIYSEDEFIKRKYSLEKFECIFDLIEEKYMDKITLKELASRVNLSAHHFCR
                     TFKQITGKTTIDFINGIRIDKAIYYLKETDLNMTEIAMRCGFDSINYFSRLFKRNYNI
                     SPTKYRNEHREVSNVILKEDMK"
     gene            1363..2790
                     /locus_tag="KPD58_002652"
     CDS             1363..2790
                     /locus_tag="KPD58_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0266405.1"
                     /translation="MTNSFKKDDNRKKITVLFVVIAMTFMATLDSSIINVALPVLASK
                     LNVSLASIEWVIASYSIIICSTLLFFGRLGDIIGKSRVFQVGTILFTSASLLCGLSNS
                     LTLLIVCRFIQGIGASAYMANNHGIITELFPKESRGKALGILVTAVAIGNMVGPSVGG
                     FILSIFDWNVIFFINIPIGLIVIFLNTKFLPNSKKSSENMDKTGAILQFLGTTLFFSA
                     LISAQQTGLLNPYILIALLLSIIFIILFLILEKKHPQPLLDLEIFRNFKFSLNLICAL
                     TSFICIASSSILIPFYLQSTMKLPPIQAGLFMILSPLILAIFSPIFGNISDKIKSEKI
                     ILIGLLVMSFGFFLMSRLKESSALILFVIYISIISIGQAIFQPANNALIMSSCSRSKL
                     GVVGSINSLVRNLGQVIGITISTTLLYNFMSIKAGYRVNDYVINNDKIFVFGMRNVYI
                     IVTLVCLIGAILIGFYLFKYNKNEQ"
     gene            complement(2986..5580)
                     /gene="clpB"
                     /locus_tag="KPD58_002653"
     CDS             complement(2986..5580)
                     /gene="clpB"
                     /locus_tag="KPD58_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBG0266406.1"
                     /translation="MDVEKMTLRVQKSLNEAYNEAVKNHNQQVDVIHLFSALINQEDG
                     LIPNIIEKMNISIDSLRNTVNFEIDKLPKVYGEGADSQGVSATRKINEVLIKAESISK
                     EFKDSYISVEHVMLAMMETESKSAVGKILKQYNINKNDFLNILSQVRGSQRVETQDPE
                     GTYDALARYGTNLVDLAKKNKLDPVIGRDEEIRRIIRILSRRTKNNPVLIGEPGVGKT
                     AIVEGLAERIVRGDVPEGLKDKIVFSLDMGALIAGAKYRGEFEERLKAVLKEVQSSDG
                     KIILFIDEIHTIVGAGKTEGSMDAGNLIKPMLARGELNCIGATTFDEYRKYIEKDKAL
                     ERRFQPVIAEEPTVEDTISILRGLKERFEIHHGVRIHDNAIVAAAKLSHRYIQDRFLP
                     DKAIDLIDEAGAMIRSEIDSLPTELDVVRRKLFTLETEREALLKENDDKSKSRLDDIQ
                     KEIAELKSKNDEMTAKYEKEKSQILDIKNLKAQLDEAKGKAEKYEREYDFNKAAEVKY
                     GEIPKLEEQIKQYEENMNDGSENSLLKEEVTEEEISSIVSKWTGIPVTKLVEGEREKL
                     LKLEDELHKRVIGQDEAVTAVSNAVIRARAGLKDERKPIGSFIFLGPTGVGKTELAKT
                     LARNLFDSEDNIIRIDMSEYMEKHAVSRLVGPPPGYVGYEEGGQLTEAVRRAPYSVIL
                     FDEIEKAHEDVFNMFLQILDDGRLTDNKGKTVDFKNTLIIMTSNIGSNYLLEAGGNIT
                     ETTNNLVMNEMKHRFKPEFLNRVDDIIMFKPLDQENIKKIIDIFMKDLKNRLKEKDIT
                     IEVTNSAKDVMVREGYDPVYGARPLKRYIGNTLETIIAKKLIAGDVYNGCTIVIDGKD
                     ENIEVLVK"
     gene            complement(5617..6213)
                     /locus_tag="KPD58_002654"
     CDS             complement(5617..6213)
                     /locus_tag="KPD58_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266407.1"
                     /translation="MSLKLDRNVLQWFDYVFENEKTSLRHYNFNCTLKEISSTSLNKV
                     AFILEKNNSKYWKLYFEIPAEVTLKLKQNIHPLFREYIYEQISLYNNNQIYNFVNSNI
                     LKVFNNIAIYQYNILENLYTIDFKKSFIDKCQYLLIGEKRLIDEDLYLIAKSKEVFDF
                     FNSDGTFNLTLSFDIQKNENLLDSLLELRKSIIINERI"
     gene            complement(6230..6634)
                     /locus_tag="KPD58_002655"
     CDS             complement(6230..6634)
                     /locus_tag="KPD58_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HBG0266408.1"
                     /translation="MDKTLKKDLNQEYTESKSLVMNKSKYMNVCFKNYIKVVEGMYTK
                     RDLIPLKLINSMDKNNKIRKTLYELSCEFEYPKTSLSSLFTELKKMDFLQRVKNGEYM
                     INPHIAYKGSRADKESLLAEYNQIKRPNKVSK"
     gene            7001..7375
                     /locus_tag="KPD58_002656"
     CDS             7001..7375
                     /locus_tag="KPD58_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0266409.1"
                     /translation="MNIVISNSSGKPIYEQITTQIKSMIISGELPEGSALPSMRLLAK
                     ELRISVITTKRAYSDLERDGFIETVTGKGSFVSSKNMDFVKEEQFRLIEEYLQKAVDT
                     AKSSDITYDELKDILFLLYKGE"
     gene            7372..8235
                     /locus_tag="KPD58_002657"
     CDS             7372..8235
                     /locus_tag="KPD58_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0266410.1"
                     /translation="MISMDAITIKNLNKTYKDFSLQDISFSVPKGSVMGFVGENGAGK
                     TTTLKAILNLISYDSGNIEIFGLDSKKNEKEIKEQIGVVFEGSNFHENLNTDHVSKIM
                     SKIYKNWNDTLFKDYLKKLRVPDNKLIKEFSKGNKMKLSIAVALSHKPKLLILDEATS
                     SLDPIVREEILDIFLDFIQDEEHSIILSSHITSDLDKIADYITFIHKGKIVFSENKDE
                     LIDTMGVLKCKPSDFDNLSREDYSYYRKSQFGYEVLLKDKHRFISRHPNCIVDNTSIE
                     EIMLFYVRGDK"
     gene            8235..8873
                     /locus_tag="KPD58_002658"
     CDS             8235..8873
                     /locus_tag="KPD58_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBG0266411.1"
                     /translation="MKGLILKDLLNLKGNIKFILLFIIMFGFMSSLGDGNVNNFIGVI
                     IVLCTTMIVSTFSYDDLNKWDSYVLTMPINRNDIVLSKYLTMLIFSFIGVLVSLIVSV
                     TIGYFKNTLILNETLLINALILSISVCFGSLILPLIYKFGTERARLLMILCFLVPTLA
                     LLVFKSILENISSPISIEIILNTLVYSLPFVAILLFVISYFISSKIYSKKEV"
BASE COUNT         2977 a         1314 c         1028 g         3584 t
ORIGIN      
        1 attttctata aaaatcatac tattttagct gtttacttgc ttatttaata gttttatttc
       61 tttgattaaa tatctttcag aatctaagcc agatgtctca aaaaaactga tttataatat
      121 aatcattttt tctgctattc ttatttttcc agttttagtt tcatattaaa atatactttt
      181 catagacact gcattctttc atactcattt tcacatttaa ctatacaagt agtcttactc
      241 atttatgtac tatactttat taatatttat taagaaaaac tcaaagattt aaaaatatat
      301 attttctata aagtatattt atggattatt tcatatcttc ttttaaaata acattactaa
      361 cttctctatg ttcatttcta tacttagttg gagatatatt ataattcctt ttaaataagc
      421 gactaaaata atttatgcta tcaaagccac atctcatagc aatttctgtc atatttaaat
      481 ctgtttcctt taaataatat atagctttat caatcctgat tccatttata aaatcgattg
      541 tagtcttacc tgttatttgc ttaaatgttc tacaaaagtg atgtgcactc aagttcacac
      601 gactagcaag ttcttttaat gtaattttat ccatatattt ttcttctatc aaatcaaaga
      661 tacattcaaa tttttctaaa gaatactttc tttttataaa ttcatcttca gaatatattt
      721 tatcaatatg acttcttaat aagagtacta ttaattgata gatattagct tttatagata
      781 actcatatcc aacttcttta ttgtgatatt catcaagtat agttgtaata cattttaaaa
      841 tcagagaatc attttgaatt aaattattaa atacaatatg atttaaagcc aatggtgcta
      901 agtacttggt ttgtaacaaa tcaacttgat tactaaaaag gaatgtgggt tcaatacgaa
      961 tactatagaa ttttaaatta tctgataagc tttctaaata atgtaattca ttactgtttg
     1021 ctattacaat atttccaggc actaccttaa aattatccaa tccacattca agatatgcta
     1081 taccatcaat aaaataatat atttgtagat gctcatgcca atgtctttta aagtttgctc
     1141 cttttttaat gtcatccttt aaaaatatat ctataaagtt ttcttgacct ttcctgttaa
     1201 aatatgtatt tttatccttc attattcccc cctcagcaaa atagtgctaa ttataagcaa
     1261 tatagttatt gtattcatta tttcatatcg ctattatata aaatgtaact atcattgtaa
     1321 acattaacat aatgcagtca ttataataag gaggtaataa aaatgacaaa ctcttttaaa
     1381 aaagatgata atagaaaaaa aatcacagtt ttatttgtag tcattgccat gacttttatg
     1441 gcaacattag attctagtat tataaatgtt gctctcccag ttcttgcaag caaactaaat
     1501 gtatctctag catctattga atgggtaata gcaagttact caattattat ttgttcaaca
     1561 ctattatttt ttggaagact tggagatatt attggtaaat caagagtatt tcaagtaggt
     1621 actattctct ttacatcagc ttcattacta tgtggattaa gtaattcttt aactttactt
     1681 atagtttgcc gctttattca aggaataggc gcttctgcct acatggcaaa caatcatgga
     1741 attatcacag aactatttcc aaaagaaagc agaggaaaag ccctagggat tctagttact
     1801 gcagttgcta ttggaaatat ggttggacca tctgtgggag gatttatttt gtcaatattt
     1861 gattggaatg ttatattttt tataaatatt ccaatcggtc ttattgtaat ctttttaaat
     1921 acaaaatttc ttcctaatag taaaaaatct tcagaaaata tggataaaac tggtgctata
     1981 cttcaatttt taggaacaac attatttttt agtgctctaa ttagtgctca acaaacagga
     2041 ttactaaatc catatatatt aattgcacta ttattatcaa ttatttttat tatattattt
     2101 ttgatattag aaaaaaaaca tcctcaaccg cttcttgacc ttgaaatttt tagaaatttt
     2161 aaattttcat tgaatttaat ttgtgctctt acatcattca tttgtattgc atcatcaagt
     2221 attttaattc cattttattt acaaagtact atgaaacttc cccctattca agcaggttta
     2281 tttatgatac tatcaccttt gattttagcc attttttcac ctatatttgg gaatatatca
     2341 gataaaatta agtcagaaaa aattatttta attggtcttc tcgttatgag ttttggattc
     2401 tttctaatgt caagattgaa agaaagttct gcccttattt tatttgtaat ttatatatcg
     2461 ataatatcaa taggacaagc tatatttcaa cctgctaaca atgctttgat aatgtcaagc
     2521 tgttctagaa gtaagctagg agttgtagga agcataaatt cccttgtaag aaatttagga
     2581 caagttatag gtattactat ttctacaaca cttttatata attttatgag tattaaagct
     2641 ggatataggg tcaatgatta tgtgattaat aatgataaaa tatttgtatt tggaatgaga
     2701 aatgtttata ttattgttac acttgtttgc ttaataggtg caatattaat tggcttttat
     2761 ttatttaaat ataataaaaa tgaacaatag tttttataat tatattcaaa ttatgattat
     2821 attataagaa atttactaaa atacttcctt tgagttaaat ttaatttgga catattgact
     2881 taaaaatgtt ttattaattt gataaaatat ttcttttcct atagtttata aataaaaatc
     2941 agctgagcaa aatatttagt atattatgct caactgattc ttttattatt taactaaaac
     3001 ttcaatattt tcatcttttc catctataac tatagtacat ccattataca catctccagc
     3061 aataagtttc tttgcaatta tagtttctaa tgtatttcct atatatctct taagaggtct
     3121 tgctccatat acagggtcat atccttctct caccataaca tcttttgctg aatttgtaac
     3181 ttcaatagta atatcttttt cttttaatct attttttaaa tctttcataa atatatctat
     3241 gattttcttg atattttctt ggtctaaagg cttaaacatt ataatatcat ctactctgtt
     3301 taaaaactca ggtttaaatc tatgcttcat ttcattcatt actaaattgt tagtagtctc
     3361 tgttatattt cctcctgctt ctaatagata attacttcct atattagaag tcattattat
     3421 aagagtattt ttaaagtcta ctgtcttacc tttattatca gtaagtctac catcatctaa
     3481 tatctgtaag aacatgttaa atacatcctc atgtgctttt tctatctcat caaatagtat
     3541 tacagaataa ggagcacgtc taactgcttc tgtaagttga ccaccctctt catatcccac
     3601 gtaccctgga ggaggtccaa ctagtcttga tacagcatgt ttttccatgt attcagacat
     3661 atctattctt attatattat cttcactatc gaataaattt ctagcaagag tttttgcaag
     3721 ttcagtttta ccaacacctg tagggcctaa gaaaataaat gaaccaattg gttttctttc
     3781 atcttttaat cctgctcttg cacgtattac tgcatttgaa actgctgtaa ctgcttcatc
     3841 ttgtccaata actcttttat gaagttcatc ttctagttta agtaattttt ctctttctcc
     3901 ttcaactagt ttagttactg gtatacctgt ccattttgat acaatgctag atatttcttc
     3961 ttcagtaact tcttctttta aaagagaatt ttcagaacca tcattcatat tttcttcgta
     4021 ctgctttatt tgttcttcta acttaggtat ttcaccatat ttaacctctg ctgctttgtt
     4081 gaaatcatat tctctttcat acttttcagc tttaccttta gcttcgtcta attgagcctt
     4141 taagttttta atatctaata tttgactctt ttctttttca tattttgctg tcatttcatc
     4201 atttttagat tttaactctg ctatttcttt ttgaatatca tccaatcttg atttactttt
     4261 atcatcattt tctttaagta atgcttctct ttcagtttct aatgtaaata actttcttct
     4321 aacaacatct aattctgttg gcaatgaatc tatttcacta cgaatcatag ctccagcttc
     4381 atcaattaaa tcaattgctt tatctggtaa aaatctatct tgtatatatc tatgagaaag
     4441 ttttgctgct gctacaatag cattatcatg tattcttact ccatgatgta tctcaaatct
     4501 ttcttttaaa cctctaagta tagatattgt atcttcaaca gtaggttctt ctgcaattac
     4561 aggctgaaaa cgtctttcaa gagccttatc tttttctata tactttctat attcatcaaa
     4621 agtagttgca ccaatacagt tcaattcacc tcttgcaagc ataggtttaa taagattacc
     4681 tgcatccatt gaaccttctg ttttaccagc tccaacaata gtatgaatct catctataaa
     4741 cagtataatt tttccatcag aactttgaac ttcttttaaa actgccttta atctttcttc
     4801 aaattcacct ctatattttg caccagcaat taaagctccc atatcaagtg agaatacaat
     4861 cttatctttt aaaccttctg ggacgtctcc tcttactatt ctctctgcta acccttctac
     4921 aattgcagtt ttacctacac ctggctcacc aatcaataca ggattatttt ttgttcttct
     4981 tgataaaatt cttataatcc ttcttatttc ttcatctctc ccaattacag ggtctaattt
     5041 atttttcttg gctaaatcta ctaagtttgt tccatatcta gcaagtgcat catatgtgcc
     5101 ttctgggtct tgtgtttcta ctctttgact acctctcact tgtgataaaa tatttaaaaa
     5161 gtcattctta tttatattat attgttttaa tattttacca acagctgatt ttgattctgt
     5221 ttccatcatt gcaagcataa catgctctac acttatatat gaatctttaa attctttaga
     5281 aatactctct gcctttataa gcacctcatt tatttttcta gtagctgata ctccttgtga
     5341 gtcagcacct tctccatata cttttggaag cttatcaatt tcaaagttta cagtatttct
     5401 caaagaatca attgatatat tcattttttc gattatattt ggtatcaatc catcctcttg
     5461 attaataagt gctgagaaaa gatgaattac atctacttgt tggttatgat tttttactgc
     5521 ttcgttataa gcctcattta aacttttttg aactcttaaa gtcatttttt ctacgtccat
     5581 ataaatccct ccattttaat attatatcca aataaattaa attctctcat ttataattat
     5641 tgattttcta agctctaaaa gactatcaag taaattctcg tttttttgta tatcgaaact
     5701 caatgttaaa ttaaatgttc catctgaatt aaaaaaatcg aatacttctt ttgattttgc
     5761 tattaaatac aagtcttcat ctataagtct tttttcacct attaataaat actgacattt
     5821 atctataaaa ctctttttaa aatcaatagt gtatagattt tctaaaatat tatattgata
     5881 aatagcaata ttattaaaca cttttaatat gtttgaattt acaaaattgt aaatttgatt
     5941 attgttatac agtgatattt gttcgtatat atattcccta aataaaggat gaatgttttg
     6001 cttaagtttt aaagtaactt cagctggtat ttcaaaataa agcttccagt acttagagtt
     6061 atttttttct aaaataaaag ctactttgtt caaactggta gaactaatct cctttagtgt
     6121 acaattaaaa ttatagtgtc tcaaactagt tttttcattc tcaaatacat agtcaaacca
     6181 ctgtagaaca tttctatcaa gttttaaact catataaaaa ccttcttcct tatttactaa
     6241 ccttattagg tctttttatc tgattatatt ctgctaataa actttcttta tcagctctac
     6301 tacctttata tgctatatgt ggatttatca tatactcacc atttttcact ctttgtaaaa
     6361 aatccatttt tttcagctcc gtaaacaatg aagataaaga agtttttgga tattcaaatt
     6421 cacaagataa ttcatataat gtttttctaa tcttattatt tttatccata gagtttatta
     6481 attttaatgg aattaaatcc ctcttagtat acattccttc tactacttta atataattct
     6541 taaaacaaac attcatatac ttacttttat tcataactaa acttttgctc tctgtatatt
     6601 cctgatttaa atcctttttt aatgtcttat ccaaattaaa tatctcttct cactctcctt
     6661 atctctaatt gataatataa atatattaca aaaaaatatg tatgtcaaaa ctaaaaatgc
     6721 aaaaagttta ccttataagt aaacttttat ttagctataa gctaaacttt attatttttt
     6781 taatttagta tttccaattt attggcagga tttttgaaca atatttataa attttttaaa
     6841 aaaatttata tctatttaat atggcagact ttcataaaat acattacatt tattaaatgt
     6901 ataatattgg caattagttt tttctaaaat cagttgacat attatatata tatttatata
     6961 atgtgtatat actatatata caatttaagg aggtgtctta ttgaatatag ttattagtaa
     7021 ttcgagtggg aagccaatat atgaacagat tactacacaa attaaaagta tgattataag
     7081 tggagagtta ccagaaggct cagctcttcc aagtatgaga ctattggcaa aagaacttag
     7141 aataagtgta attactacaa aacgagcata tagcgattta gaacgagacg gatttattga
     7201 gacagttact ggtaaaggca gttttgtatc tagtaaaaac atggattttg tcaaagaaga
     7261 acaatttcgt ttaattgaag aatacctaca aaaagcagtt gatacagcaa aaagtagtga
     7321 tattacatat gacgaattaa aagatatttt atttttatta tataaaggag aatgataagt
     7381 atggatgcaa taactataaa aaatttaaat aaaacttata aagattttag cctacaagat
     7441 atatcttttt cagtaccaaa agggagtgta atgggatttg taggtgaaaa tggtgcagga
     7501 aaaacaacta cattaaaagc aattttaaat ttaatatctt atgatagtgg aaatatagaa
     7561 atctttggtc ttgatagtaa aaaaaatgaa aaagaaatta aagagcaaat tggggttgta
     7621 tttgagggaa gcaactttca tgaaaattta aacactgacc atgtttcaaa aatcatgagt
     7681 aaaatctata aaaattggaa tgacacatta tttaaagact acttaaaaaa attgagagta
     7741 ccagacaata aattaattaa ggagttttca aaggggaata agatgaaact tagtattgct
     7801 gtagccttat ctcataaacc aaaattatta atactagatg aggcaactag ttcacttgac
     7861 cctatcgttc gtgaagaaat actagatata tttttagatt ttatacaaga tgaagaacac
     7921 tctattatct tatcttcaca tattacaagt gatttagata agatagcaga ctatattaca
     7981 tttatacata aaggaaaaat tgtatttagt gaaaataagg atgaattaat tgatactatg
     8041 ggagttttaa aatgtaaacc tagtgatttt gataaccttt caagagagga ttattcatat
     8101 tatcgtaaaa gccaatttgg atatgaagta cttcttaaag ataagcatag atttatctca
     8161 agacatccaa actgtattgt tgataatacg tctatagaag aaattatgtt attttatgtg
     8221 agaggtgata aataatgaaa ggtctaattt taaaagattt acttaatttg aaaggaaata
     8281 taaagtttat acttttattc atcattatgt ttggctttat gtcttcttta ggagatggaa
     8341 atgttaataa ctttataggt gttattatag ttttatgtac tacaatgatt gtttcaacat
     8401 tttcatatga tgatttgaat aaatgggata gttatgtact tacaatgcct attaatcgca
     8461 atgatattgt attaagtaaa tatttaacta tgttaatatt tagctttata ggtgttcttg
     8521 tttcacttat tgttagtgta actattggtt attttaaaaa tacacttata ttaaatgaaa
     8581 cactacttat taatgcacta atcttatcaa tttcagtatg ttttggtagc cttattctac
     8641 cacttattta taaatttggt acagaaagag ctcgtttatt aatgatttta tgctttttag
     8701 ttccaacact agctttatta gtttttaaaa gtattttaga aaatattagt tcacctattt
     8761 ctatagaaat tatacttaat acactagtat attctcttcc atttgttgct attttactat
     8821 ttgtgatttc atattttata tcatcaaaaa tctatagtaa aaaagaagta taaaatatac
     8881 tatatatgtt tctgtattta agt
//
LOCUS       DAELLV010000154         8890 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.154, whole genome shotgun sequence.
ACCESSION   DAELLV010000154 DAELLV010000000
VERSION     DAELLV010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8890
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.154"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            74..1063
                     /locus_tag="KPD58_002659"
     CDS             74..1063
                     /locus_tag="KPD58_002659"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBG0266412.1"
                     /translation="MFENLPKIDLHCHLDGSVRVETMLDIAIKEKIDLPSNNMDEIKK
                     LAKVSFNCTSLDEYLEKFDLPLKVMQSKENLKRITFELLEDASRENVKYIEIRFAPLL
                     HTQKGMSVKNIIEGIIEGIREAESIYDIKGNLILGCMRTMTSKEALLVIEEGKSFVNK
                     GVVAVDLCGPEKEGFCKEYKDVFKLAREYGYKVTIHAGEAASGENVLDAINILKADRI
                     GHGVKIKDHKKAYNLVKDKKILLELCPTSNVQTKTVDSYEAHPFYTFYKDNLHVSINT
                     DNRTVSDINLSSELNVIFDTFKLGLEDYKIIYRNAVEASFADKETKEYLNSLI"
     gene            1135..1875
                     /locus_tag="KPD58_002660"
     CDS             1135..1875
                     /locus_tag="KPD58_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HBG0266413.1"
                     /translation="MKDYEDVVYIKLVKKELEEWKKNILKKPSIFDKASKGIQNKFNG
                     VLPDKYHEIITSSIKVMTKTVLFGYKYATKPPLRNMSLEEREKLVLEKAKTYKTTAMI
                     EGAGTGAGGILLGLSDFPLLLGIKVKFLYDVASIYGYDVKDYRERIYILNILQLAFSS
                     QQHINKTFKLMNNWDEYVNSLPEDINDFDWRTFQQEYRDYLDLAKFLQLMPGIGAFVG
                     FYVNGKLIDKLCETAVSAYRLRIRELKL"
     gene            2091..3242
                     /locus_tag="KPD58_002661"
     CDS             2091..3242
                     /locus_tag="KPD58_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HBG0266414.1"
                     /translation="MTNLPKQFDSFNEARQKGFINAKELKESGKKMVGVFCTFTPVEI
                     PMAAGATVVGVCGVSEEPIPDAERVLPRNLCPLIKSSYGHAITDTCPYFYFSDLLIGE
                     TTCDGKKKMYEELAKVKPTYVMHLPNTSKGEFAYKLWKDEMIRLKEEVEKSLGVTITE
                     EDIRTAIKDKNEERELLKEFYALGKLQPSALTGLELHNVLYQAGFKFDRAELKHSLRK
                     VIDDMKERYEKGECPVQKDKPRILITGSPIGGISEKIVKTLEDAGASVVAYELCGAIR
                     SNDLLVDEEIEDVYDALTQKYINIGCSCMMNNDNRIELLDRIIDEYNVDAVIDVVLQA
                     CHTFNIESYRIREFVTKEKNKPFMSLETDYSKSDTEQLRTRFEAFVEML"
     gene            3267..4022
                     /locus_tag="KPD58_002662"
     CDS             3267..4022
                     /locus_tag="KPD58_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HBG0266415.1"
                     /translation="MFSIGVDSGSVATKGVLFDGEKIIKKIIIPTGWSPKSTSKQVYE
                     LLSSEIDKKDIKKVVGTGYGRGVMDFADKKVTEITCHTRGIYFLNKNIRTILDVGGQD
                     SKVINLDRDGNVFNFIMNDKCAAGTGRFLEITSNLLGSDIESIDTLAKGYEPVNISSM
                     CTVFAESEIVSLLAQNISTGEVAAGILKSIANKSTSMLARGEVIDEVAFTGGLAKSKE
                     LVKMIEEILGKKIFIAEDTQIIGALGAAVIGFR"
     gene            4214..6601
                     /locus_tag="KPD58_002663"
     CDS             4214..6601
                     /locus_tag="KPD58_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp13"
                     /protein_id="HBG0266416.1"
                     /translation="MKKFTSKKVTRFVALFLISILVMSTIPVSADNSNTLNQIKKAEY
                     SETYKQYLNDAKNGCTEKYNGIIPNPYQLEGTQIQSKGRTAPTSYDPRKLGLMTSIKN
                     QEDLGICWDFAAMATLESFLKLNNYGDYDLSEEHLRWWASDGEYGWSVNDMNGALNYE
                     AMGYLTSWSGPKLEKDIPYNGRVSKAQGAKKPTNMNTAPTVFNVTDAVCVSNDINSTK
                     NAILQYGAVTSGYYEDIKYQSDDQNSYYCPTKSFNTNHAISIVGWDDNYSKDNFNANI
                     RPSKNGAWLIKNSWGDYNSEGGYFWISYEDKTLMSDIDNFSIKGGKKPNDDEKMYQHD
                     YASIVPLISKKITAANVFDFNRGDETLKSVMFLTESIGAKYEVYYAPVVNGIPQENNM
                     KKLKEGTAQYSGYITVPIDSFDIPEGKGAIVVSIEGKNGESTIGSESNVPGYDTFKAK
                     ANLGESYIIDNTGKFFDINRDSNFYPCNFTIKAVTEKSSGESIPNESLIGSDRYETAI
                     KVSQNGFNSSENVVLVNGSSIVDALAATPFTSAINSPILLTQKEALNSKTKAEIQRLG
                     AKKVYLIGGENSISKEIEQQLKSLNISIERISGSDRYKTSLLLAQKLNGIKNVSQIAV
                     VNGVKGLADAISVGAAAAENNIPIILANEKSELQGADEFLNSLNIEKSYIIGGTASLS
                     NNLESKLKNPTRLSGSSRDETNSKIIDNFYKKDTLKNAFVVKNGIKNQNDLIDGLAVG
                     PLGAKTGSPVILVGDKLADSQKEVLKNKTLEKVTQVGGGANKNAFKELVKLKSAK"
     gene            complement(6832..7065)
                     /locus_tag="KPD58_002664"
     CDS             complement(6832..7065)
                     /locus_tag="KPD58_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBG0266417.1"
                     /translation="MATTTKNRFYDYVQENLNKYVEVHSYFSSCPLYGEIIEADSLSI
                     TLCSRYIDESNPTLNETYTLYLPLSSIISIRTL"
     gene            7354..7944
                     /locus_tag="KPD58_002665"
     CDS             7354..7944
                     /locus_tag="KPD58_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0266418.1"
                     /translation="MPKSYSDKEREYIIKRLKEEARLCMEQYGIRKTTVDELVKRVKI
                     PKGTFYLFFQSKELLFFEVLRDIHDSIQKEILYEINRVDESITCEQLTDIFMKFYRMV
                     DSTSILNLMINGEFEILVRKLPDSIIEEYFRHDDFEIGEIISCIPNAKNKDIESFSGA
                     FRAVFLTMLYKREVGSNCFEDALRLMINGLVIQLME"
     gene            8058..>8890
                     /locus_tag="KPD58_002666"
     CDS             8058..>8890
                     /locus_tag="KPD58_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0266419.1"
                     /translation="MIKVDDLSFSYTDRDFLQNINFEVGKGEILGFLGPSGAGKSTLQ
                     KILIGMITNYGGSVIVNGVESKRHSNKFYENIGVDFEFPSLYEKLTAIENLKYFGSLY
                     SKKLLSIDELLKSVGLENESNKRVSEYSKGMKSRLNFIKALLHNPDILFLDEPTSGLD
                     PSNSKVMKDIILSEKSKGKTIILTTHNMLDATELCDRVAFIVNGKISALDTPHNLIMS
                     KGAIKVRYTYFDNGEKTSECFLNNTANDKNLNMLIEKNKLLSIHSSEPTLNDIFIEIT
                     G"
BASE COUNT         3598 a          900 c         1587 g         2805 t
ORIGIN      
        1 tatattgata taatagaaat aagacttaaa aatctgttga tagtattatt tataagtaca
       61 gtaggaggat aaaatgtttg aaaatttacc taagatagat ttacattgcc acttagatgg
      121 aagtgttaga gtagaaacca tgcttgatat agctattaaa gagaaaatag atttaccttc
      181 aaataatatg gatgaaataa aaaaattagc aaaggtttct tttaattgta cttcactaga
      241 tgaatattta gaaaaatttg atttaccctt aaaagtaatg caatctaagg aaaatttaaa
      301 aagaattact tttgaacttt tggaagatgc aagtagagaa aatgtaaaat atattgaaat
      361 tagatttgca ccattacttc acacccaaaa aggtatgagt gttaaaaata taattgaggg
      421 tataattgaa ggaattagag aagctgaaag tatttatgat ataaaaggca atttaatatt
      481 aggttgtatg agaacaatga cctcaaaaga ggcattatta gtaattgaag aagggaaatc
      541 atttgtaaat aaaggtgttg tagcagtaga cttgtgtggt ccagagaaag agggattttg
      601 taaggaatat aaagatgttt ttaaattagc aagagaatat ggatataaag ttacaataca
      661 tgcaggagaa gctgcaagtg gagaaaatgt attggatgct ataaatattt taaaggcgga
      721 taggattggt catggggtaa aaattaaaga ccataaaaag gcgtataatt tagttaaaga
      781 taaaaaaata ttgcttgaat tatgtccaac tagtaatgta caaactaaga cagtagattc
      841 ttatgaagca catccatttt atacttttta taaagataat ttacatgtaa gtataaatac
      901 tgataataga actgtatcag atataaattt aagttcagaa ctaaatgtta tatttgatac
      961 ttttaaacta ggattagaag actataaaat aatttataga aatgcagtag aagctagttt
     1021 tgcagataaa gaaactaaag agtatttgaa tagtttaatt taatagataa aaatatttaa
     1081 actttaaatt aagctattaa attgataaaa atacattgta ttaggagaaa aaatgtgaaa
     1141 gattatgaag atgttgttta tattaaacta gtcaaaaaag aacttgaaga atggaagaaa
     1201 aatatattaa agaaaccttc tatatttgac aaagcatcaa agggtataca gaataaattt
     1261 aatggagtat tgccagataa atatcatgaa ataattactt cttctataaa agtaatgaca
     1321 aaaaccgtac tgtttggata taaatatgct acaaagccac cacttagaaa tatgtcatta
     1381 gaagaaagag aaaaattagt tttagaaaaa gctaaaactt acaagactac agcaatgatt
     1441 gaaggggcag gaacaggggc aggaggaatc ctcttaggtt tatctgattt tcctttgctt
     1501 ttaggtatta aagtgaaatt tttatatgat gtagcttcaa tatatggata tgatgttaaa
     1561 gattatagag aaaggattta tatattaaat atattacagt tggctttttc tagtcaacag
     1621 catataaaca aaacttttaa attgatgaat aactgggatg aatatgtaaa tagtctacca
     1681 gaagacatca atgattttga ttggagaaca tttcaacaag agtataggga ttatttagat
     1741 ttagctaagt ttttacaact aatgcctgga ataggagcat ttgtaggatt ttatgtgaat
     1801 ggaaaattaa ttgataaatt atgtgaaact gctgtgtctg cttatagatt gaggattcga
     1861 gagcttaagt tataagaaaa attataacat aaaaaattag ttatacaata tatctatatg
     1921 tatctaattt tgcattgata aaaacaatta tggataaaag gtaaagcatg atagaatcta
     1981 atgtagaggt ttgtatttta atggaaaaat ttagttttta ttgcttaaga tacaaattga
     2041 attacgaaga gattgaatta tgaagagaag taattgggag gaatattttc atgacaaatt
     2101 tgccaaaaca atttgatagc ttcaatgaag ctagacaaaa aggatttata aatgcaaagg
     2161 aacttaagga aagtgggaaa aagatggttg gagttttctg tacatttact ccagttgaaa
     2221 ttccaatggc agctggagca actgtagtag gtgtatgcgg cgttagtgaa gagccaatac
     2281 ctgatgcaga aagggtgctt ccaagaaatc tttgtccttt aattaaatca agctatggac
     2341 atgcaataac agatacctgt ccttatttct atttttctga tttgttgata ggagaaacca
     2401 cttgtgatgg taagaaaaaa atgtatgaag aacttgcaaa agttaagcca acatatgtaa
     2461 tgcatttgcc aaatacttca aaaggagaat ttgcctataa actttggaaa gatgaaatga
     2521 taagattaaa agaagaggtt gagaaatctt taggtgtaac tataactgaa gaagatataa
     2581 gaactgctat aaaagataaa aatgaagaaa gagaactttt aaaagagttt tatgcacttg
     2641 gtaaattaca accatctgca cttacaggcc ttgagttaca taatgtttta tatcaagcag
     2701 gttttaaatt tgaccgtgca gaacttaaac attctttaag aaaagttata gatgatatga
     2761 aagaaagata tgaaaaaggt gaatgtcctg ttcaaaaaga taagccaaga atattgataa
     2821 caggttctcc tattggtgga ataagcgaaa aaatagttaa aactttagaa gatgcagggg
     2881 catcagtcgt tgcatatgaa ctttgtggag ctattagatc aaacgattta ttggttgatg
     2941 aagaaataga agatgtatat gatgctttaa cacaaaaata tataaatata ggttgttctt
     3001 gtatgatgaa taatgacaat cgtattgaat tattagatag aataatagat gaatataatg
     3061 tagatgctgt aatagatgta gttttacaag catgtcatac atttaatata gaaagttata
     3121 gaataagaga atttgttaca aaagaaaaaa ataagccatt tatgtctctt gaaactgatt
     3181 attcaaaatc agatactgaa caattaagaa ctagatttga agcgtttgta gagatgttgt
     3241 aggaataaat aaaagtagga ggaactatgt ttagtatagg agttgactca gggtcagtgg
     3301 caacaaaggg tgtgctattt gatggtgaaa aaattattaa gaaaataatt ataccaactg
     3361 gatggagccc taaaagtaca tctaaacagg tctatgagtt gctaagtagt gaaattgaca
     3421 aaaaagatat aaaaaaggtg gtaggtacag gttatggaag gggagtaatg gattttgctg
     3481 ataagaaagt aacagaaata acttgtcata ctagaggtat ttacttttta aacaagaaca
     3541 taaggactat tttagatgta ggtggtcaag atagtaaagt tataaattta gatagagatg
     3601 gaaatgtatt taactttata atgaatgata agtgtgctgc aggaactggt cgatttttag
     3661 agattacttc taacctttta ggaagcgata ttgaaagtat agatacatta gctaaaggat
     3721 atgaaccagt taatatatct agtatgtgta cagtttttgc tgagtctgaa attgtaagtc
     3781 ttctagctca aaacatttct actggagagg tagcagcagg gatacttaaa tctattgcaa
     3841 ataaatccac ctcaatgtta gcaagaggtg aggtaataga tgaagttgca tttactggag
     3901 ggcttgcaaa aagtaaagaa ctagtaaaaa tgatagaaga aatactgggt aaaaaaattt
     3961 ttattgcaga agatactcaa ataataggag ctttaggagc tgctgtaata ggatttagat
     4021 aaaaacatat aaaaagaaaa tgagaagctg aatttattat aaataaattc agttttttat
     4081 tttattttat ttatttataa aggaaaactt tggtataatt gatttaaatg attgtatatt
     4141 tgtaaatact tgatatttag tcatatataa cattataatt ttaaaaatta gctcaaaata
     4201 agggggagaa agcgtgaaaa aatttacttc aaaaaaagta acaaggtttg ttgcgctgtt
     4261 tttaatatct attttagtaa tgtcaacaat tcctgtatca gctgacaata gcaatacatt
     4321 gaatcagata aaaaaagcag aatacagtga gacgtataaa caatatctaa atgatgctaa
     4381 aaatggatgt acagaaaaat ataatgggat tataccaaat ccatatcagt tggaaggtac
     4441 tcaaatccaa agtaaaggaa gaacagctcc aacatcttat gaccctagaa aattaggtct
     4501 tatgacatca ataaaaaatc aagaggattt aggaatctgt tgggattttg ctgcaatggc
     4561 aaccttagaa agtttcttaa agttaaataa ttatggagac tatgatttat cagaagaaca
     4621 cttaagatgg tgggcttcag atggtgaata tggatggtct gtaaatgata tgaatggtgc
     4681 tttaaattat gaagctatgg gatatttaac atcttggtca ggtcctaaat tagaaaaaga
     4741 tatcccatac aatggtagag tatctaaagc acaaggagca aaaaaaccaa ctaatatgaa
     4801 tacagcacct actgttttta atgtgacaga tgctgtgtgt gtaagcaatg acataaattc
     4861 tacaaaaaat gctatattac agtatggagc tgtaacatct ggttattatg aagatattaa
     4921 atatcaaagt gatgaccaaa attcatatta ttgtccaaca aaaagtttta atacaaatca
     4981 tgctatttca attgtaggtt gggatgataa ctattctaaa gataatttta atgcaaatat
     5041 tagaccatca aaaaatggtg cttggttaat aaaaaatagc tggggagatt ataattctga
     5101 gggtggatat ttctggatat cttatgaaga caagacactt atgtctgata tagataattt
     5161 ttcaatcaaa ggtggtaaaa aaccaaatga tgatgagaag atgtatcaac atgactatgc
     5221 aagtattgta cctttaattt caaaaaagat aacagctgca aatgtatttg atttcaatag
     5281 aggcgatgaa actctaaaat ctgtaatgtt cttaactgaa agtataggag ctaagtatga
     5341 agtatattat gctccagtag taaatggtat acctcaagaa aacaatatga aaaaattaaa
     5401 agaaggtact gcccaatatt caggatacat aacagttcca attgattctt ttgatatacc
     5461 agaaggaaag ggtgcaattg tagtcagtat agaggggaaa aatggagagt caactatagg
     5521 ttctgagtca aatgtaccag gatatgatac atttaaagca aaagctaatt taggtgaaag
     5581 ttatatcata gataatactg gaaaattctt tgatataaat agagatagta acttctatcc
     5641 gtgtaatttt actataaaag ctgttacaga aaagtcttct ggtgagagta tcccaaatga
     5701 gtctttaatt ggtagtgata gatatgaaac tgctatcaaa gttagtcaaa atggtttcaa
     5761 ttcttcagaa aatgtagttt tagtaaatgg cagttcaata gtagatgcat tggcagcaac
     5821 tccttttaca tctgctataa attcaccaat attattgaca caaaaagaag ctttaaattc
     5881 aaaaactaaa gctgagatac agagattagg tgctaagaaa gtatacctaa taggtggaga
     5941 aaattctata agtaaagaaa ttgaacaaca attaaaaagc ttaaatatat cgatagagag
     6001 aatatcagga agtgatagat acaagacaag tttattatta gctcaaaagt taaatggtat
     6061 aaaaaatgta tctcaaatag cagttgtaaa tggagtgaaa ggattagctg atgcaattag
     6121 tgtaggcgct gcagcagcag aaaataacat accaatcata cttgcaaatg aaaagagcga
     6181 attgcaagga gctgatgaat ttctaaattc attaaatata gagaaatctt atattattgg
     6241 aggaacagca tccctatcaa acaatctaga aagtaagtta aaaaatccaa ctagattatc
     6301 tggtagtagt agagacgaaa ctaattcaaa gataatagat aatttttata aaaaagatac
     6361 tttaaaaaat gcatttgtag ttaaaaatgg aatcaagaat caaaatgatt taatagatgg
     6421 attggcagtt ggtcctttag gagcaaaaac tgggtcacca gtaattttag ttggtgataa
     6481 attagctgat agccaaaaag aagttcttaa aaataagact ttagaaaaag taactcaagt
     6541 tggtggagga gcaaataaaa atgcatttaa agagttagta aaattaaaaa gtgctaaata
     6601 gagtacttaa gtattaacat atattagtgt gtattagtaa cttatcttaa taaaagctga
     6661 aaataattac ttcaaattat tgataaataa gataatcaat gattgtaaag ttttgttttc
     6721 agctttaaat ttatgtagta taagtttaaa aacataaaaa agggttactt tatatagaaa
     6781 tatagaaaat cttacatttt atatataaat aacccatatt taattaactt tttatagtgt
     6841 tctaatagat attattgaac ttaatggtaa atataatgtg taagtttcat ttaaagttgg
     6901 attagattca tctatatatc ttgagcaaag tgtaattgat aagctatctg cttctataat
     6961 ttcaccatat agaggacaac tgctaaagta agaatgtact tcaacatatt tatttaagtt
     7021 ttcttgaaca taatcgtaaa aacggttttt tgtagtagta gccataaaat caactccttt
     7081 attttttatt aaagttgaaa cttttaaaaa gataaatatg attacttcta ttagaaacta
     7141 agttaaaaaa taagcaaata gatatgaaaa ttagaataat atattattta tatcttaatt
     7201 ataacactag ataataaaaa atgctatata aaattcagaa ttttcgaaag tttttaagct
     7261 tatttttaaa tatactaata atactattga cattagatag aaaaagtatt atattgacta
     7321 tataaattat tttagtcaaa ctggagggaa aagatgccta aatcatattc tgataaagaa
     7381 agagagtata taataaaaag acttaaagaa gaagcaagat tgtgtatgga gcaatatggt
     7441 attagaaaaa ctactgttga tgagcttgta aaacgggtaa aaataccaaa aggaactttt
     7501 tatctatttt ttcaatcaaa agaattactc ttttttgagg tattaagaga tatacatgac
     7561 tctatacaaa aagaaatctt atatgagata aatagagtag atgaaagtat tacatgtgaa
     7621 caattgacag atatatttat gaaattctat aggatggttg atagtacttc aattttaaat
     7681 ctcatgataa atggagaatt tgagatattg gtgagaaagt taccagattc tataattgaa
     7741 gaatacttta gacatgatga ctttgaaatt ggagagatta tatcttgcat accaaatgca
     7801 aaaaataaag acatagaaag ctttagtgga gcatttagag cagtttttct taccatgcta
     7861 tataagcgcg aggttggaag taattgtttt gaagatgctt taagattaat gataaacgga
     7921 ttagttatac aacttatgga atagaattta aattttgttt gctaataact tttattaaaa
     7981 attattaaca aacaaaaaaa ttatatttta ttgaccatat agtcaaaata gtcaaaaatt
     8041 tgaaagggtg atattgaatg attaaagttg atgatttatc atttagttat acagatagag
     8101 attttttaca aaatattaac tttgaagtag gaaaagggga aatactagga tttttaggac
     8161 catcaggtgc aggaaaatct acattacaaa agattctaat tggaatgata actaattatg
     8221 gaggaagtgt cattgtaaat ggagtagaga gtaaaagaca ctcaaataaa ttttatgaaa
     8281 atataggagt tgattttgaa tttcctagtt tatatgaaaa gttgactgca atagaaaatt
     8341 taaaatattt tgggtctcta tattctaaaa aactactatc tattgatgag ttattaaaat
     8401 cagtaggact agaaaatgaa tcaaataaaa gggtatctga gtattcaaaa ggtatgaaat
     8461 ctcgattaaa ttttataaaa gctttattac ataatccaga tattttattt ttagatgaac
     8521 caacaagtgg tcttgaccca tcaaatagca aggttatgaa agatataatt ttatcagaaa
     8581 aatcgaaagg taagactatt attttaacaa ctcataacat gttagatgca acagagcttt
     8641 gtgacagagt agcgttcatt gtaaatggta aaatatctgc tttagataca ccacataatc
     8701 ttattatgtc aaaaggagct attaaagtta gatatactta ctttgataat ggagaaaaaa
     8761 cttctgaatg ttttctgaac aatactgcta atgataaaaa tttgaatatg cttattgaga
     8821 aaaataaact tttatcaata catagtagtg aaccaacatt aaatgatatt tttattgaga
     8881 taacagggag
//
LOCUS       DAELLV010000155         8781 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.155, whole genome shotgun sequence.
ACCESSION   DAELLV010000155 DAELLV010000000
VERSION     DAELLV010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8781
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.155"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            23..370
                     /locus_tag="KPD58_002667"
     CDS             23..370
                     /locus_tag="KPD58_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266420.1"
                     /translation="MKKNYKIHNLNEYKNKKKNNYKKRNKKKIGAKCILLIATFSVII
                     LNLCGYSKISEMKYDIYYLQKDLRKKEIVLEELKAKETEKTSIKEIELKAKELLNMDY
                     PKDGQIKYIDVDN"
     gene            382..2361
                     /locus_tag="KPD58_002668"
     CDS             382..2361
                     /locus_tag="KPD58_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HBG0266421.1"
                     /translation="MRKVKRISKKRLVLVLILACALFFCLVIRTGYLQLMKGNWLSTK
                     ALEQQTRDIPIEPKRGTIYDRNMKELAVSVTKYTVWCKPVEVEDKKEAAEKVAEILDE
                     DYKDIYALISKKNMALVKVKRWIDDDKASQIRDAKLSGIWVAEDNQRYYPYGNFAPYV
                     LGHTSSDATGISGVEMQYDKKLKGKPGKLIVSTDASGREIPQGMEKYYEPVQGNGLVL
                     SIDEVIQHYTEKAVQKAYELNNAKKVTAIAMNPKTGDILALASKPDYDPNDSRTPIYP
                     YYQEELEKYNDKDKIKGYYQMWRNPAVSDTYEPGSTFKLITSSSALEEGVIKDGEKFT
                     CTGSVTVGGRKIKCWRHYRPHGTQEFKQAVQNSCNPVFVELGSRLGVGKMYDYIESFG
                     LMDKTGIDLPGEAKGILYNEKNVGPVELATISFGQSISVTPIQLITAISSIANGGDLM
                     QPRVVKSYTDNKGNITETVKPKKVRSVISKETSKKMLEIAESVVTEGGGKIAYIPGYR
                     LGGKTGTAQKVIDGKYAPGKYICSFVGIAPCDDPQIVVLAIVDEPTGVSAFGSTTAGP
                     IVKEIMNDSLKYLGVKPVYKEEEKAEYEKKQVKVPDVRNLKIGDAVKALEDAKLKPDL
                     DADIELPEDTKVKDIFPKPGVKVNEDSSITLYFEN"
     gene            2528..3901
                     /locus_tag="KPD58_002669"
     CDS             2528..3901
                     /locus_tag="KPD58_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBG0266422.1"
                     /translation="MECLTIKELVLATNGKLIYGDYNDCVSDIVIDSREANAQNAFVA
                     IVGENLDGHTFMKSAYDSGCKTFIKNESNGIKLESSDINLIEVKDTSLALGDISKYYK
                     EKFDIPFIGVTGSVGKTTTRDMIYSAISAKLNILKNEKNLNNHFGVPLTLFNLNKEHE
                     CAVIEMGMSGFNEIKYLVDIVNPKIAVISNIGLSHVEKLGSQEGILKAKMEITSNFDE
                     TNTLIVNGDDKFLSTLKEKEHVYKLKTFGFNKNNDVYCESYTMEEDSLTFICVINGKK
                     EEIFIPTIGEHNIYNAMSAILVGLCLNISLDYIKKGLKNFKGTKMRLDIIKNEKLTII
                     NDSYNASPDSMDAALKILGRYKGRRVAILGDILEMGEISEYGHRLVGKSSMNNTDIII
                     TIGENSVFIGEEAKQLGFNSANIYHFENREDVFNKLNELVKTGDTILVKGSRGMRLEK
                     IVEYLNK"
     gene            3942..4910
                     /locus_tag="KPD58_002670"
     CDS             3942..4910
                     /locus_tag="KPD58_002670"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBG0266423.1"
                     /translation="MMLGITELTYTALIAFLIVIIIGPIFIPMLRKFKFGQTVRDDGP
                     QTHLAKNGTPTMGGIIMIVAILITGLTRVKVSHDMAVGLICIAGFGFIGFLDDFIKIK
                     LKRSLGLKAYQKIILQVALSFYVAFYQYTSSSSASQLMIPFTDFVINVGILYIPIMMF
                     IIVAIVNAVNLTDGLDGLASGVTLIVSVFFMLFASSIAGNTEVAVLAAATVGACLGFL
                     GFNSYPARVFMGDTGSMALGGAVVAFSVLTNSVLIIPIIGGIYFAEALSVLIQVGYFK
                     ATRKRFFKMAPIHHHFEQCGWPETRVVFIFWIITVVLAWISIIAVF"
     gene            4929..6284
                     /locus_tag="KPD58_002671"
     CDS             4929..6284
                     /locus_tag="KPD58_002671"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBG0266424.1"
                     /translation="MDLKGKKVLLVGLAKTGISTIKHLDKLGASIIVNDIKDENKLRN
                     ILDELKSINDIKYILGHHPEDVDDIDMVVVSPGVPLDLPFILKLKNSGKYIIGEVELA
                     FKLSNNPIFIGITGTNGKTTTTSLVGEIFSRAKRDTYVVGNIGNPVIDTIETSSEESV
                     LVTELSSFQLESIDEFRPKVSAILNITEDHLNRHHTMEKYIEAKANIFMNQTVEDFCV
                     LNYDDEIVKSLADKCNAKVIYFSRTKKVNGGVYLENNDIIIDIDDKIKFLNKDDVSLP
                     GGHNLENCMAAIAIAYVCKIDLEVIKDVLMTFKGVEHRQEFVRNLDNVIYVNDSKGTN
                     PDSTIKAIQSYDRPIILIAGGMDKGSNFDELLETAKSYVKSLVLLGETASNIENCAKN
                     KGFNDIHIVKDMEEAVKTSYEISKSGDIVLLSPACASWDMYESFEVRGKDFKDNVNNL
                     K"
     gene            6336..7466
                     /gene="spoVE"
                     /locus_tag="KPD58_002672"
     CDS             6336..7466
                     /gene="spoVE"
                     /locus_tag="KPD58_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein E"
                     /protein_id="HBG0266425.1"
                     /translation="MPKENLKKQIDIRMKTEFDGVVFYTTMLLVFVGIVMVFSASFIQ
                     SSFKHNDAYYFLKKNVIYAVLGFIVMIITSRIDYSFWKKNATAIGAIAVVLLLLVLTP
                     LGIEANGAKRWLGIGALTFQPAEIAKFATIILTAKLIEKNYDKIKSLTKGVVPLLVVP
                     GIFFALIILQPNLSTAGTVILVTFVMIFVAGMDMKIVFAMIGSGAALFAALVIAEPYR
                     LSRVTSFLDPFQDPLGKGYQVIQGLYALGSGGLFGLGLGKSKQKYFYIPEPQNDFIFA
                     IIGEELGLIGCIIVIMLFVVLVYRCVRIALKTSNVFACMVVIGIGAQIGIQAALNIAV
                     ATSSMPATGVALPFISYGGTSLTIFMGAVGIVLNISKHVKIN"
     gene            7484..8710
                     /gene="murG"
                     /locus_tag="KPD58_002673"
     CDS             7484..8710
                     /gene="murG"
                     /locus_tag="KPD58_002673"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBG0266426.1"
                     /translation="MKVLLSGGGTGGHVYPAIAIANKIRDEHPDAEIIFVGTEKGIES
                     EIVPKYGFELKTVTVQGFKRKIDFDNVKRVFKLFKGLEQSRKIVKKFKPDIVIGTGGY
                     VSGPVLFNASMGKIPAIIHEQNSFPGVTNKILSKTVTKVLTSFEDSHKRFPEAAEDKL
                     VFTGNPVRKEILLSRKNIARKNLSISDEKRMVLCYGGSGGSRKINDAMRLVIKNMVNE
                     DIAFIFATGKSYYDEFMGSISDINLKPYQKVVPYLEDMANALAASDLVIGSAGAISLA
                     EITALGKPSIIIPKAYTAENHQEYNAKSIEKQGAGIAILEKNLTPESLNTAVFKLLGD
                     RELLVDMANASKTIGKPEAIDLIYDEIMKVYNSTQKSASKKTKKEKVIKEVKEIKKET
                     TPSIEGQAKVIGIKKR"
BASE COUNT         3464 a          993 c         1712 g         2612 t
ORIGIN      
        1 tatatactat cggggtgaga atttgaaaaa aaattataaa atacataatc taaatgaata
       61 taaaaataaa aaaaagaata attataaaaa gagaaataag aaaaaaatag gagctaaatg
      121 cattttactg attgcaactt tttcagtaat aatattgaat ctatgtggtt attcaaaaat
      181 ctcggaaatg aagtacgata tctattattt acaaaaagat ttaagaaaaa aagagatagt
      241 attagaggaa ctaaaggcaa aagaaactga aaaaacatca ataaaagaaa ttgagctaaa
      301 ggctaaggaa ttgctcaata tggactatcc aaaagatgga caaataaagt atatagatgt
      361 ggataactag ggggaataga tttgagaaaa gtaaagagga taagtaagaa aaggctggta
      421 ctcgttctta ttctagcatg tgcattgttt ttttgccttg ttattaggac tgggtatcta
      481 caacttatga agggaaattg gttgagtaca aaagcactag aacaacaaac aagagatata
      541 ccaatagaac ccaaaagagg aactatatat gatagaaata tgaaagagtt agcagtaagt
      601 gtaactaagt atactgtttg gtgtaaacct gtggaagtag aagataaaaa agaagcagcc
      661 gaaaaggtag ctgagatttt ggatgaagat tataaagata tttacgcttt aataagcaaa
      721 aaaaatatgg ctcttgtaaa agtaaaaaga tggatagatg atgataaggc aagtcaaata
      781 agagatgcta aattaagtgg aatatgggtg gcagaagata atcaaagata ttacccatat
      841 gggaattttg caccatatgt gttaggacat acttcttcag atgcaacagg tatatctggt
      901 gtagaaatgc aatatgataa aaagttaaaa ggaaagcctg gaaaacttat agtaagtact
      961 gatgcttcag gaagagaaat accacaagga atggaaaaat attatgagcc tgtacaaggt
     1021 aatggtcttg tgttgtctat agatgaagta attcaacact atactgaaaa agcagtacag
     1081 aaagcttatg agttaaataa tgcaaaaaag gttactgcaa tagctatgaa ccctaaaaca
     1141 ggtgatatac ttgctttagc atcaaaacca gattatgacc ctaatgactc aagaacgcca
     1201 atatatccat attatcaaga agaacttgaa aaatataatg ataaagataa aataaaaggc
     1261 tattatcaaa tgtggagaaa tccagcagta agtgatacat atgaaccagg ttctacattt
     1321 aagcttatta catcttccag tgctcttgaa gaaggagtaa ttaaagatgg agaaaaattc
     1381 acatgtacag gtagtgtaac tgttggagga agaaagatta aatgttggag acattacaga
     1441 cctcatggaa cacaagaatt taaacaagca gtacaaaact cttgcaaccc agtatttgtt
     1501 gagttaggaa gcagattagg tgttggaaaa atgtatgatt atatagaatc atttggtctt
     1561 atggataaga caggtataga tttaccagga gaagctaagg gaatactgta taacgaaaaa
     1621 aatgtaggac cagttgaatt ggctacaata tcatttggtc aatctatatc agtaactcca
     1681 atacaactta taacagctat atcatcaata gctaatggtg gagatttaat gcaaccaaga
     1741 gtggtaaaat cttatactga caataaagga aatataactg aaacagtaaa accaaaaaaa
     1801 gtaagaagtg ttatatctaa agaaacatct aaaaaaatgt tagaaattgc agaatcagtt
     1861 gtaactgaag gtggaggaaa gatagcatat ataccaggtt atagacttgg tggtaaaaca
     1921 ggtacagctc aaaaagttat agatggtaag tatgctccag gaaaatatat atgttcattt
     1981 gttggaatag caccttgtga tgacccgcaa atagtagttc ttgctattgt tgacgaacca
     2041 acaggagtaa gtgcatttgg tagtactaca gcaggaccaa tagtgaaaga aataatgaat
     2101 gactctttaa aatatttggg agttaaacca gtatataaag aagaagaaaa agctgagtat
     2161 gaaaagaagc aagttaaagt gccagatgta agaaatctaa aaataggaga tgctgtaaag
     2221 gcattggagg atgcgaagtt aaaaccagac ttagatgcag atatagaact tccagaagat
     2281 acaaaggtaa aggatatatt cccaaaacct ggagtaaaag ttaatgaaga ctcaagtata
     2341 accctatatt ttgaaaacta aaaagtaaca gataattaac atatacccat aaacatcttc
     2401 aaaaaaacag aaatatagta tataattata aagttaagag ttatcgtagg caatgtatat
     2461 ataacggtat acattgcctt ttgttgctaa gagaatatag ctaaggtttt aaaggaggag
     2521 tttaataatg gagtgcttaa caataaaaga gttagtactt gcaactaatg ggaagttaat
     2581 atatggagat tataatgact gtgtaagtga tatagttata gatagcaggg aggcaaatgc
     2641 ccaaaatgca tttgtagcca tagttggaga aaatttagat gggcatacat ttatgaagtc
     2701 tgcatatgat agtggatgta aaacttttat aaaaaatgaa agtaatggaa taaaattaga
     2761 aagttcggat ataaatttaa tagaggtaaa agatacatct ttggctctag gagatatttc
     2821 aaaatactat aaagaaaaat ttgatattcc atttataggt gttacaggta gtgtgggaaa
     2881 aactactact agagatatga tttactctgc catatctgca aagttaaata tactaaaaaa
     2941 tgaaaaaaac ttaaataatc attttggagt tccattgact ttatttaatc taaataaaga
     3001 acatgagtgt gctgtaatag agatgggtat gtctggattc aatgaaataa aatatttagt
     3061 ggatatagta aatcctaaaa ttgcagtcat atcgaatata ggattatcac atgtagaaaa
     3121 actaggttca caagagggta ttttgaaagc aaagatggag attacttcaa actttgatga
     3181 aacaaatact ttaattgtaa atggagatga taagttttta tctacattaa aagaaaaaga
     3241 acatgtatat aagttaaaaa catttggctt taataaaaac aatgatgttt attgtgaaag
     3301 ttatactatg gaagaggata gtttaacatt tatatgtgta ataaatggga aaaaagagga
     3361 aatatttata ccaacaatag gagaacataa tatatataat gcaatgtctg ctatattagt
     3421 tggtttgtgt ctaaatattt ctttagatta tataaaaaaa ggtttaaaaa attttaaagg
     3481 gacaaaaatg agactagaca taataaaaaa tgaaaaatta acaataatta atgattcata
     3541 taatgctagt ccagattcta tggatgctgc tttaaagata cttggaagat ataaaggtag
     3601 aagggtagcg atacttggag atatactaga aatgggtgaa atttcagagt atggtcatag
     3661 attagttgga aaatcatcta tgaataatac tgatattata ataactatag gtgaaaattc
     3721 agtgtttatt ggagaagaag caaaacaatt aggattcaat tcagccaata tatatcactt
     3781 tgagaataga gaagatgtgt ttaacaagtt aaatgaatta gtaaaaacag gagacactat
     3841 tttagtaaaa ggctcaagag gcatgagatt agaaaaaata gtcgaatatc taaataaata
     3901 agcttaaact aatgagttaa ttgacaaggg agaggagttt tatgatgtta ggaataacag
     3961 agcttacgta caccgcattg attgcgtttt taatagtaat tataatagga ccaatattta
     4021 taccaatgct tagaaaattt aaatttggtc agacagttag agatgatgga ccccaaacac
     4081 atctagctaa aaacggaaca cctactatgg gtggaattat aatgattgtt gcaattctaa
     4141 taactggact tacaagagta aaagttagtc atgatatggc agtaggtctt atatgtattg
     4201 caggttttgg ttttattggc tttttagatg attttataaa aataaagcta aaaagatcac
     4261 ttggactaaa agcatatcaa aaaataatcc ttcaagttgc attatctttt tatgttgctt
     4321 tttatcagta tacatcatca tcaagtgctt cacaacttat gataccattt acagattttg
     4381 tgataaatgt tggaatatta tatataccaa taatgatgtt tataatcgta gcaatagtaa
     4441 atgctgtaaa cttgactgat ggattagatg gattagcatc aggagtaaca ttaatagttt
     4501 ctgtattctt tatgctattt gcaagttcta ttgctggaaa tacagaagtc gcagttttag
     4561 cagcagcaac tgttggagca tgtttaggtt tcttaggatt taattcatat ccggctagag
     4621 tgtttatggg cgacacaggt tcaatggcat taggaggtgc agttgtagca ttttcagttc
     4681 taactaactc agtgcttata atacctataa ttggaggaat ttattttgcg gaggctctat
     4741 cagttttgat tcaagttgga tattttaagg caacaagaaa gagattcttt aagatggctc
     4801 caatacacca ccactttgaa cagtgtggtt ggccagaaac aagagttgta tttatttttt
     4861 ggattataac agttgtttta gcttggataa gtataatagc agtattttag ggctaggggg
     4921 aatgaaaaat ggacttaaaa ggaaaaaaag ttttattagt aggacttgca aaaactggta
     4981 tatctacaat taaacattta gataaattgg gtgcaagtat aatcgtaaat gatataaaag
     5041 atgaaaataa attgagaaat atacttgatg aattgaaatc tataaatgac ataaaataca
     5101 tattaggaca tcacccagaa gatgtagatg atatagatat ggttgtagtg tctccaggtg
     5161 taccactaga cctaccattc atattgaaat taaaaaattc aggcaagtat ataattggtg
     5221 aggtagaatt agcttttaaa ctatcgaata atccaatttt tatagggatt acaggtacaa
     5281 atggaaagac aactacaact agtttggtag gagaaatttt ttcaagagca aaacgagata
     5341 catatgtagt tggtaatata ggaaatcctg taatagatac tatagaaact tcaagtgaag
     5401 agtctgtgtt agtaactgaa ctaagcagtt ttcagcttga gagtatagat gaatttagac
     5461 ctaaagtaag cgcaatatta aatattacag aggaccattt aaatagacac catactatgg
     5521 aaaaatacat agaagcaaag gcaaatatat ttatgaacca aactgttgag gatttctgtg
     5581 tattaaacta tgatgatgag atagtaaaat ctttagctga taaatgcaat gccaaagtaa
     5641 tttacttttc aagaactaaa aaagtaaatg gaggagtata tttagaaaat aatgacataa
     5701 taatagatat agatgataaa atcaaatttc taaataaaga tgatgtaagt ttacctggag
     5761 gtcataactt agaaaattgt atggcagcta tagcaatagc atatgtctgc aaaattgatt
     5821 tagaagttat aaaggatgta cttatgacat ttaaaggagt ggaacacaga caagagtttg
     5881 taagaaattt agacaatgta atatatgtaa atgattcaaa gggaactaat ccagattcca
     5941 ctataaaagc tatacagtct tatgatagac ctataatact tatagcaggg gggatggata
     6001 agggaagtaa ttttgatgaa cttcttgaaa ctgcaaagtc atatgttaaa tcattggtct
     6061 tattaggaga aactgcatca aatatagaaa attgtgctaa aaataaagga tttaatgaca
     6121 tacatatagt aaaagatatg gaagaagcag ttaagacttc ttatgaaatt tcaaaaagtg
     6181 gagatatagt gcttctttca ccagcttgtg caagttggga tatgtatgaa agttttgaag
     6241 taagaggaaa agacttcaaa gataatgtaa acaatttaaa ataatctctt acctctaaac
     6301 ttacttaaat ggaagaatgg aggtagatta gttggatgcc taaagaaaat ttgaaaaaac
     6361 aaattgacat aaggatgaag actgaatttg atggagtagt gttttacact actatgcttt
     6421 tagtatttgt aggaatagtt atggtgttta gtgcaagttt tatacaatct tcatttaaac
     6481 acaatgatgc gtactatttt ctaaaaaaga atgtaatata tgctgtactt ggatttatag
     6541 ttatgattat tacatcaaga atagattata gtttttggaa gaaaaatgct acagcaattg
     6601 gagctatagc agtagtatta ttattgttag ttttgacccc tttagggata gaagcaaacg
     6661 gagcaaaaag atggttggga ataggagctt taacatttca acctgcagaa atcgcaaagt
     6721 ttgctacaat aatattaact gcaaaattaa ttgaaaaaaa ttatgataaa ataaaatctt
     6781 tgacaaaagg tgttgtacca cttctcgtag ttccagggat attttttgcg ttgataatac
     6841 ttcagccaaa tttgtcaaca gcaggtactg taattctagt tacttttgta atgatatttg
     6901 ttgctggaat ggatatgaaa attgtatttg ctatgatagg gagtggggct gctttatttg
     6961 ctgcattagt aattgctgaa ccctatagac ttagtcgtgt tacatcattt ttagaccctt
     7021 ttcaagaccc tcttggtaag gggtatcagg taatacaagg tctatatgca ctaggttcag
     7081 gtggattgtt tggtctaggc ttaggaaaga gtaaacaaaa gtatttttat ataccagagc
     7141 cacaaaatga ctttatattt gctataatag gagaagagtt aggattgatt ggttgtataa
     7201 tagttattat gctatttgta gttttagtct acagatgtgt aagaatagct ttgaagactt
     7261 ctaatgtatt tgcttgtatg gttgttatag gtataggtgc acaaattgga atacaagcag
     7321 ctttaaatat agcagttgcg acatcatcaa tgccagctac aggtgtagca ctaccattta
     7381 taagttatgg aggaacttca ctcaccatct ttatgggagc tgtaggaata gtgttaaaca
     7441 tctcaaagca tgtcaaaatt aattaacagg agatgatgat ttgatgaaag ttttattatc
     7501 aggcggtgga actggagggc acgtgtatcc agctatagct attgcaaaca aaataagaga
     7561 tgagcatcca gacgctgaga taatttttgt tgggacggaa aagggaatag agtcagaaat
     7621 agttcctaag tatggatttg aattaaagac tgtaacagtt caaggtttta agcgaaaaat
     7681 agatttcgat aatgtaaaaa gagtttttaa gctttttaaa ggtttggagc aatcaagaaa
     7741 gattgttaag aaatttaaac cagatatagt tataggaact ggtggatatg ttagtggacc
     7801 tgttttattt aatgcatcta tggggaaaat accagccata atacatgagc aaaattcttt
     7861 tcctggtgta actaataaga ttttatctaa aacagttaca aaggtactta caagttttga
     7921 agattcgcat aaaagatttc cagaggcagc agaagataaa ttagttttta caggaaatcc
     7981 agttagaaaa gaaattttat tatcaagaaa aaatattgca agaaagaatc tatcaataag
     8041 tgacgaaaaa agaatggtac tttgctatgg tggtagtgga ggttcaagaa aaataaatga
     8101 tgcaatgaga cttgtaataa agaatatggt aaatgaagat atagctttta tatttgctac
     8161 aggcaaaagc tattatgatg aatttatggg aagtattagc gatataaatc ttaaaccata
     8221 ccaaaaagtt gtgccttact tagaagatat ggcaaatgca cttgcagcaa gtgatttggt
     8281 tataggtagc gcaggagcaa tttcattagc tgagataacg gctcttggaa agccatctat
     8341 aataatacct aaagcttata ctgctgaaaa tcatcaagag tataatgcca agagtataga
     8401 aaaacaaggg gcaggaattg ctattttaga aaaaaatcta actccagaga gcctaaatac
     8461 agcagtattt aaacttttag gagatagaga acttttggtt gatatggcaa atgcaagtaa
     8521 aactataggt aagcctgaag ccatagacct tatatatgat gagataatga aagtatacaa
     8581 ttcaacacaa aaatcagctt ctaaaaaaac aaaaaaagaa aaagttatta aggaagtaaa
     8641 agaaataaaa aaagaaacga ctccaagtat agaaggtcaa gctaaggtaa taggaataaa
     8701 gaaaagataa gtaaatattt acccctaatt tataatatat tagattttct aaattattat
     8761 aaattagggg tttatttgta t
//
LOCUS       DAELLV010000156         8773 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.156, whole genome shotgun sequence.
ACCESSION   DAELLV010000156 DAELLV010000000
VERSION     DAELLV010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8773
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.156"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            194..961
                     /locus_tag="KPD58_002674"
     CDS             194..961
                     /locus_tag="KPD58_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBG0266427.1"
                     /translation="MGEIINALDRALDIILLLYHEKREMGITEISKAMGVYKSTVHRT
                     LVTLENKGFVIQNAENGKYWLGINLYAIGMVVGEKMSLTEIVKPYTKKLNQEFNEVVN
                     VSILEERAQDSPRSIIIHKEYGSNQLLSVNPSVGSSSECYCSAVGKCLMAFNDSIDFE
                     KYRKTPIHKYTEHTIDNWDDMMLFLAKIKEQGYAIDDEELEHGLTCIGAPILDKNNKA
                     IAAISLSGPTIRMREGDFEYKIKRVIETAKSISELFR"
     gene            1244..1441
                     /locus_tag="KPD58_002675"
     CDS             1244..1441
                     /locus_tag="KPD58_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG0266428.1"
                     /translation="MEKVKLSVERCKGCYLCIEACKKDAISLSDYTNKKGFVTIKVDE
                     EKCIQCGACYTMCPDLVFEIL"
     gene            1470..2546
                     /gene="vorB"
                     /locus_tag="KPD58_002676"
     CDS             1470..2546
                     /gene="vorB"
                     /locus_tag="KPD58_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBG0266429.1"
                     /translation="MQSLLIKGNEALAEGAIRGGCRFYAGYPITPQSEILEMMAWRQK
                     EVGGVFIQGESEIASVNMVMGASALGARAMTSSSGPGFSLKQEGIAYWVSAGLPAVVA
                     CVQRYGIGDGFIGAGQDNYWQAVKGGGNGDYHLIVLAPNSVQENMDMAYESFELAEKY
                     MHPVLILSDATIGQMVEPCIAPPMKEYDMDKAPWAAKGTPVGEKNKVITDITYFEDQD
                     TWQKGYIEKMRKVQEDEQRWEEIEIEDAEVVFVAYGISSRIAEGAVRDARAKGMKLGL
                     IRPKTLWPYPRKAFKKLSDSLKGLVTVEMNMMAQMKEDVIIACDNRFPVYSVATAQYM
                     AEVNQLIDFAQQIIDGKAKQEEVF"
     gene            2549..3268
                     /locus_tag="KPD58_002677"
     CDS             2549..3268
                     /locus_tag="KPD58_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBG0266430.1"
                     /translation="MAKKAPAIMMCENMFCGGCGHSIVDRIIGEVLTEMDICQDTIIC
                     SDIGCNHMFQFSMNVDVVVPPHGKMGAAMAAMKRVRPDKYIFTIAGDGGAYAIGLGET
                     MSAATRNDNVIFLVMNNNMFAMTGGQMAPTTIEGQYTASTPAGRNYADHGSTFDVVKV
                     MGNLDIAYLARGTITNPKEINTTKRYIKKALEKQKEKKGFCLIEVLVPCPTNWGLKPV
                     DAMKRIENELVPKYPLGEFID"
     gene            3281..3820
                     /locus_tag="KPD58_002678"
     CDS             3281..3820
                     /locus_tag="KPD58_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBG0266431.1"
                     /translation="MLNEIICAGFGGQGVLTAGKIIMYVAYKAEKKITWFPSYGNEMR
                     GGSANCNVVVSDEKIANPCVKNPDILLGMNSVSVDKYESLMKKNGYMFVNESLVSKDR
                     KYREDINVIKVPVTEIAQNLGNEKAANICMLGAMVKSLDLFTKEEFESGMCEYFENQG
                     KGKFNSKNVEAFEAGYDYE"
     gene            3977..5035
                     /gene="buk"
                     /locus_tag="KPD58_002679"
     CDS             3977..5035
                     /gene="buk"
                     /locus_tag="KPD58_002679"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBG0266432.1"
                     /translation="MKVLVINPGGTSTKISVFQDENEILKKNITHTREDLKNFSKVFD
                     EYDYRKQLIVDILSSENHSINSFDAVVGRGGLMKAIKGGTYTVSEEMIEDMRNEINGE
                     HASNLGALLAKTIADEIGVQSFVVDPVSVDEFDDVSRITGISDIEKASWLHALNHKAV
                     SRKIAQKLGGKYEDYNFIVAHLGSGISIVAHKQGKMIDGSGGRSDGPFSPERSGGLLA
                     YPLIELCYSGKYTHDEMVAKVSSIGGMYDYLGTKDMIEIENRIKDGDEKAKLIMDAFI
                     YQVAKEISMYGASLSGNVDRVILTGGISHSEMVVKGVAEKVSYLAPIEIVPGEMEMEA
                     LALGALRVLNGEEIAKTY"
     gene            5053..5946
                     /locus_tag="KPD58_002680"
     CDS             5053..5946
                     /locus_tag="KPD58_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HBG0266433.1"
                     /translation="MLKDFNNLLEEAKKLEPVIVSVAVAEDKEVLKAVKSATDLNIIK
                     PILVGDKDKIESISREIGLKGHEIIGCQNPDESVKKAVEIVKNKEAKVLMKGLVNTSV
                     YMRAILNKETGLRTGRLLSLLAVYELPQYHKLLYCTDSGINVSPNLNQKKDIMTNALL
                     TMKSIGMENPKTAILTANEMVDPKIQSTVDANLLVEMEKSGEIPKCIVEGPIAFDVAF
                     DAHAAEHKGIKSNIAGDVDLLVFPNIEAGNILGKSWLRFNKAKWAGIVLGATNPIILG
                     SRSDTAEIKINSIALACLASQ"
     gene            5976..7184
                     /locus_tag="KPD58_002681"
     CDS             5976..7184
                     /locus_tag="KPD58_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG0266434.1"
                     /translation="MKYAKRMDMVKASAIRDSQKKIAQKVASGGTVISFAAGLPDPNL
                     FPIEEISEITQKVLNERGKFALAYGPTKGEDELLDLLVKRMKEEENIDTKAENIIITT
                     GSQQGIALSAMILLEKGDVVVTENPSYLGAINAFRPYECDFLGVDTDEEGMVTDDLDK
                     LLSENSNIKVIYVIPTFQNPTGKTWSLQRRKDFMEVVNKYDVIVIEDNPYGEIRFTPE
                     PLPTLKSLDTKDKVLYLGSFSKVLCPGFRVAWMCGNVDIIDKAELLKQGIDLQSNQFS
                     QVQVIEFLKKYNLNEHIEKIRAEYKKRCLLMLDAMKKHFPEEAKYTEPDGGMFIWVEL
                     PESINVDDLLDKAIDAGVAYVSGESFFANNGPKNTMRLNYTTMSEEQIVKGVEILAEV
                     IKKELNYACE"
     gene            7208..8467
                     /locus_tag="KPD58_002682"
     CDS             7208..8467
                     /locus_tag="KPD58_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0266435.1"
                     /translation="MGEKENGIKFGKLMFQLVIIAISWELVYIIPFIQYTLYDPILKA
                     LECTNTQLGFLLTIYGLGNIFGAPIGGWLADRFDYRKIILGSVFLNGVVSFLFAFNMN
                     YPFAVATWVGCAITSLVMNYPSMVKILRVIGKDNQGKVYGFNEAMVGVSGVVMGAIFL
                     YIYTCFATPTLGMRWVMISLGILSIAMCPVLWFVIKDVDIKEEREEAGEKMSAGDFIT
                     VLKSPNTWLVGISIFCVYSFTVTMSYFTPYITSVLGGSVALSGALAIIRQHGLKLFGA
                     PFGGYCADKLKSPTKVLLPIYVFGIAVIVLFLVLPASTPMTIFIALTFVVGILGYMGK
                     GIYYAVQDEVKVPVKYSATTIGIAAALGFSPDVFQFALIGHWIDTYGNKGYTYTFIFQ
                     IAILVIGILSCLYILKVKKRRASKLEA"
BASE COUNT         3411 a          971 c         1742 g         2649 t
ORIGIN      
        1 aacagaataa ttctattaaa agtaagaata ataaggacat agtgagtatt ttgtttttat
       61 tttacaaatt aagtataata tttttgtttt ataaaatata attaagataa atagataatt
      121 ttgtaatttt aatatataat atttagtaga gtgtgggagt agcacttaat atttgcagga
      181 ggagtttttg tatatgggag aaataataaa tgcattagat agagccctag acataatttt
      241 gctgttatat catgaaaaga gagaaatggg aataactgaa ataagtaagg ctatgggagt
      301 atataaaagt actgtacata gaactttggt tactcttgaa aacaaaggtt ttgttataca
      361 aaatgcagag aatggtaagt attggctagg aataaattta tatgcaatag gtatggttgt
      421 gggagaaaaa atgtcattga ctgaaatagt aaaaccatat acgaaaaaat taaatcaaga
      481 atttaacgaa gtagttaatg tatctatatt agaagaaaga gctcaagatt ctccaagaag
      541 tatcataata cataaagagt atggttcaaa tcaattactt tcagtaaatc catccgttgg
      601 ttcaagtagt gaatgttatt gttcagcagt tggtaaatgt cttatggcat ttaatgattc
      661 tatagatttt gaaaaatata gaaaaacacc aatacacaag tatacagaac atacaattga
      721 taattgggat gatatgatgc tttttttagc aaaaataaaa gaacaaggat atgctataga
      781 tgatgaagaa ctagaacatg gactaacttg tattggagca cctatcttag ataaaaataa
      841 taaagctatt gcagccataa gtttgtcagg tccaactata agaatgagag aaggggactt
      901 tgagtataag attaaaagag ttatagaaac tgcaaaaagt atatctgaac tttttagatg
      961 atttttaatc tcgataataa aaataggcta tatttaaatt gttatttgta aatatcagtt
     1021 taaatgtagc ctatttttct ttaatctaaa attaaatatt cttaaaattt aaaatgttat
     1081 tgaaatatat aagcgaatgt gttatctttt aattaagcaa taaaaataga acaaggttct
     1141 atactataga acaaaattga tgcatatata gtatttatac aaaatataaa tattatatat
     1201 ttaaaattaa gtaaaaatat tacataaagg ggagatttga gaaatggaaa aagtcaagtt
     1261 gagtgttgaa agatgtaagg gatgctatct ttgtattgaa gcttgtaaaa aagatgcaat
     1321 aagtctttct gactacacaa ataaaaaagg ctttgtaact attaaagtcg acgaagaaaa
     1381 atgtattcaa tgtggcgctt gttatacaat gtgccctgat ttagtatttg agatattata
     1441 aattattttt attagagggg agaaaaggta tgcaaagttt attaattaaa ggaaatgaag
     1501 ctttagcaga aggtgctata cgtggtggct gcagatttta tgcaggttat ccaataacac
     1561 cacaatcaga aatattagaa atgatggcat ggagacaaaa agaagttgga ggagtattta
     1621 tccaaggtga atcagaaata gccagtgtaa atatggttat gggagcaagt gcacttggag
     1681 caagagctat gacttcttca tctggtccag gattttcatt aaaacaagaa ggtattgcat
     1741 attgggtatc agctggtttg ccagcagttg tagcatgtgt gcaaagatat ggtataggtg
     1801 atggatttat aggagctggt caagataatt attggcaagc agtaaaaggc ggtggaaatg
     1861 gagattatca tttaatcgtg ttagcaccta atagtgttca agaaaatatg gatatggcat
     1921 atgaatcttt tgaattggct gaaaaatata tgcatccagt acttatactt tctgatgcta
     1981 caataggtca aatggtagaa ccttgtatag caccaccaat gaaagaatac gatatggata
     2041 aagctccatg ggctgcaaaa ggaacacctg taggagaaaa aaataaagta ataactgata
     2101 taacatattt tgaagaccaa gatacatggc aaaaagggta tatagaaaaa atgagaaaag
     2161 ttcaagaaga tgaacaaaga tgggaagaga tagaaataga agatgctgaa gtggtatttg
     2221 tggcatacgg tatttcatca agaatagcag aaggtgcagt tagagatgct agagcaaaag
     2281 gaatgaaatt aggtctaata agaccaaaaa cattgtggcc ttacccaaga aaagcattta
     2341 agaaattaag tgattcattg aagggacttg taacagttga aatgaacatg atggcacaga
     2401 tgaaggaaga tgtaattata gcatgtgata atagatttcc agtatattca gtagcaacag
     2461 ctcaatatat ggcagaagta aatcaattaa tagattttgc tcaacagatt atagatggaa
     2521 aagctaagca agaggaggta ttttaattat ggctaaaaaa gcaccagcaa taatgatgtg
     2581 tgaaaatatg ttctgtggag gctgtggaca ctctatagtc gatagaatta ttggagaagt
     2641 attaacagaa atggatatat gtcaagatac aattatatgt tcagatatag gttgtaatca
     2701 tatgtttcaa tttagtatga atgtagatgt tgtcgttcca cctcatggta aaatgggagc
     2761 agcaatggca gctatgaaaa gagttagacc agataaatat atatttacaa tagctggaga
     2821 tggtggagct tatgcaatag ggcttggaga aactatgtca gctgctacta gaaatgataa
     2881 tgtaatattt cttgttatga acaataatat gtttgcaatg acaggagggc aaatggctcc
     2941 aactactata gaaggtcaat atacagcttc tactccagcg ggtagaaatt acgcagatca
     3001 tggttcaaca tttgatgttg taaaagttat gggaaattta gatatagcat atctagcaag
     3061 aggaacaata actaacccta aagaaataaa tacaacaaaa agatacatca aaaaagctct
     3121 agaaaaacaa aaagaaaaga aaggtttttg cttaatagaa gtgcttgtac catgtccaac
     3181 aaactgggga ttaaagccag tagatgctat gaagagaatt gaaaatgaac ttgttccaaa
     3241 gtatccactt ggagaattta ttgattaggg aggaaataat atgttgaatg aaattatatg
     3301 tgctggtttt ggaggacagg gagttcttac agcaggaaaa attataatgt atgttgcata
     3361 taaggcagaa aagaaaatta catggttccc atcttatgga aatgagatga ggggaggaag
     3421 tgcaaattgc aatgttgttg taagtgatga aaaaattgca aatccttgtg taaaaaatcc
     3481 agatatacta ttgggaatga acagcgtatc agtagataag tatgaaagtt taatgaaaaa
     3541 aaatggttat atgtttgtca atgaatcact agtaagtaag gatagaaagt acagagaaga
     3601 cattaatgtt ataaaagtac ctgttactga aatagcacaa aatttaggca atgaaaaagc
     3661 tgctaacatt tgcatgttag gtgctatggt aaagagtttg gatttattta caaaagaaga
     3721 atttgaaagt ggtatgtgtg aatactttga aaatcaaggc aaaggtaaat tcaattctaa
     3781 aaatgtagaa gcatttgaag ctggatacga ctacgaatga atattttaaa tatttaaaat
     3841 aattattgaa atacttattt ttatatgtta gtatttaatt aataaaatat agaacgtcgt
     3901 tctggacaat gggacaaagt ctttaggtcg gaatacaaga acgacgttct gcatagtaga
     3961 actagggggt gttaaattga aagttttagt aataaatcct ggtggtacat ccacaaaaat
     4021 ttctgttttc caagatgaaa atgaaatttt gaaaaaaaat ataacccata caagagaaga
     4081 tttaaaaaat ttttctaaag tatttgatga atatgattat agaaaacaac tgatagtaga
     4141 tattttaagt agtgagaatc atagtataaa ttcttttgac gcagtagttg gtcgtggtgg
     4201 acttatgaag gctataaaag gtggtaccta tactgtgagt gaagaaatga ttgaggatat
     4261 gagaaatgaa ataaatgggg aacatgcatc aaatttaggt gcattattag ctaaaactat
     4321 agctgatgaa ataggagtac aatcatttgt agtagaccca gtttctgtag atgaatttga
     4381 tgatgtatcg aggattacag gaatatcaga catagaaaaa gcaagttggt tacatgcatt
     4441 aaaccataaa gctgtttcaa gaaaaattgc acaaaaacta gggggaaaat acgaggatta
     4501 taactttatt gtagctcatt taggttcagg aatatcgatt gtagcacata aacaaggcaa
     4561 aatgattgat ggaagtggtg gtaggtctga tggtccattt tctccagaaa ggagtggtgg
     4621 attacttgca tatcctctta tagaattgtg ttactccgga aaatatactc atgatgaaat
     4681 ggtcgcaaaa gtaagttcga ttgggggaat gtacgattat ttaggaacaa aggacatgat
     4741 agaaattgaa aacagaatca aggatggtga tgaaaaggca aaacttataa tggatgcatt
     4801 tatttatcaa gtagcaaaag aaatttctat gtatggagct tcactttcag ggaatgtaga
     4861 tagagttatc ttgacaggag ggatatctca ctcagaaatg gtagtcaaag gagtagcaga
     4921 aaaagtttca tatctagctc caatagagat agtaccagga gagatggaaa tggaggcact
     4981 tgctctaggg gcacttagag tacttaatgg agaagaaata gcaaaaactt attaatattt
     5041 aggagaaaga gtatgttaaa agattttaat aatttattag aagaggctaa aaagcttgaa
     5101 cctgttattg taagtgttgc tgttgcagaa gacaaagaag tattaaaggc tgtgaaatca
     5161 gcaacagatt taaacattat aaagccaatt cttgtaggag acaaggataa aatagaaagt
     5221 atatcaagag aaattggatt aaaaggtcat gaaataatag gttgtcaaaa tcccgatgaa
     5281 tcagttaaaa aagctgtaga gatagttaaa aataaggaag ctaaagttct tatgaaggga
     5341 ttagtaaata caagtgtcta tatgagagct atattaaata aagaaacggg tcttagaaca
     5401 ggaagattat taagtttgct tgcagtatat gaattacctc aatatcataa acttctttat
     5461 tgtactgata gtggaataaa tgtatcgcca aacttaaatc aaaaaaaaga tataatgaca
     5521 aatgcacttc ttactatgaa aagtataggc atggaaaatc ctaaaactgc gatacttact
     5581 gcaaatgaaa tggtagaccc taagatacaa tctacagtgg atgccaacct acttgtagag
     5641 atggaaaaat cgggagaaat accaaagtgt atagtggaag gtccaatagc atttgatgta
     5701 gcatttgatg cacatgcagc agaacataaa ggtattaaaa gtaatattgc tggtgatgtt
     5761 gatttattag tatttccaaa tatagaagca gggaatatat taggaaagtc atggcttaga
     5821 tttaataaag ctaagtgggc aggtatagtt ttaggagcaa caaatccgat aattttaggt
     5881 tcacgttctg acacagctga aataaaaata aattcaatag cattagcgtg cctagcatca
     5941 caataagaaa ttattattaa ggaggaaaaa gtattatgaa atatgcaaaa agaatggata
     6001 tggtaaaagc ttcagctata agagattctc aaaagaaaat agctcagaag gtagcaagtg
     6061 gtggaactgt tatatctttt gctgcagggc ttcctgaccc aaatttattt ccaatagaag
     6121 agataagtga aataactcaa aaagtactta atgaaagggg aaaatttgcc ctagcatatg
     6181 gacctactaa aggtgaagat gagttgttag atctattagt aaaaagaatg aaagaagaag
     6241 aaaatattga tacaaaagca gaaaatatta taataactac tgggtcacaa caagggatag
     6301 cattaagtgc tatgatttta ttagaaaagg gagatgtagt agttacagaa aatccaagtt
     6361 acttaggtgc aataaatgca tttagacctt atgaatgtga ttttttagga gtagatacag
     6421 atgaagaagg aatggtaaca gatgatttag acaaattatt atctgaaaat tctaacataa
     6481 aggtgatata tgtaatacca acatttcaaa accctacagg aaaaacatgg tctttacaaa
     6541 gaagaaaaga ttttatggaa gtcgtgaaca aatatgatgt catagtaata gaggataatc
     6601 cttatggaga aataagattt acaccagaac ctctacctac acttaaatct cttgatacta
     6661 aagataaagt attgtacctt gggtcatttt caaaggtatt atgtccagga tttagagttg
     6721 cttggatgtg tggtaatgta gacatcatag ataaggcaga acttttaaaa caaggtatag
     6781 atttacagtc aaatcaattt tcgcaagtac aagtaataga atttttaaag aaatacaact
     6841 taaatgaaca tatagaaaaa ataagagcag agtataagaa aagatgtctg ctaatgttag
     6901 atgctatgaa gaaacatttc ccagaggaag ctaaatatac agaaccagat ggaggaatgt
     6961 ttatatgggt tgaacttcct gaaagtataa atgtagatga tttattagat aaagccattg
     7021 atgctggagt tgcttatgtt tctggagagt ccttctttgc aaataatggt ccaaaaaata
     7081 ctatgagact taactataca acaatgtcag aagaacaaat cgttaaaggt gttgagatac
     7141 ttgcagaagt tataaaaaaa gaactcaatt atgcttgtga ataaatataa aaagtgaggg
     7201 tttaaatatg ggagaaaaag aaaacggtat aaagtttgga aagttaatgt ttcaacttgt
     7261 tattatagca atatcttggg aattagttta tattatacca tttatccaat atacattata
     7321 tgatccaatc ctaaaagcat tagaatgtac aaacactcaa ctaggttttc tacttacaat
     7381 ttatgggtta ggtaatatat ttggagctcc tataggaggc tggttagcag atagatttga
     7441 ttatagaaag ataattttag gttctgtatt tcttaatgga gtagtatcat ttttatttgc
     7501 atttaatatg aactatccat ttgcagttgc tacttgggta ggttgtgcaa tcacatcatt
     7561 agttatgaac tatccttcta tggtaaaaat acttagagtt ataggaaaag ataatcaagg
     7621 taaggtttat ggatttaatg aagctatggt tggagtttct ggagtagtaa tgggagcaat
     7681 attcttatat atttatacat gctttgcaac tcctacattg ggtatgagat gggtaatgat
     7741 atctttagga atattatcaa tagcaatgtg tccagtatta tggtttgtaa ttaaagatgt
     7801 agatattaaa gaagaaagag aagaagcagg tgaaaaaatg tctgctggtg attttataac
     7861 agtactaaaa tcaccaaata cttggctagt aggaataagt atattctgtg tatattcatt
     7921 cacagttaca atgtcttatt ttacaccata tataacatct gtacttggag gttctgtagc
     7981 attatctggt gcattagcaa taataagaca gcatggtcta aaattatttg gagcaccatt
     8041 tggaggatat tgtgcagaca aattaaaatc tcctacaaaa gtacttttac caatatatgt
     8101 atttggtata gcagtaatag ttttattcct agtattacct gcatcaactc caatgactat
     8161 attcatagct ttaacatttg tagtaggtat attaggttac atgggaaaag gtatatatta
     8221 tgcagtacaa gatgaagtaa aagttccagt taaatattca gcaactacta taggtatagc
     8281 tgcagcttta ggattttcac cagatgtatt ccaatttgca ttaataggac actggataga
     8341 tacttatggc aataaaggtt acacatatac atttatattc caaatagcaa ttctagttat
     8401 tggaatacta tcttgtttat atatattaaa ggttaaaaag agaagagcaa gtaaattaga
     8461 agcttaaata ttcaatttgg taattaagta tatataaata caatacttat tttgatgaat
     8521 gtgattgtat aaataacata aaaaatatca atagattaag ttttataata atgtttagag
     8581 aataaagtat aaaaatcaaa atgttatcaa gttttctagg ttttaacata aagttacccc
     8641 tgaaggtgta aaattgagtg gcaattatta tctattttct tacctacata aacctattta
     8701 atttaaattc taagagattt tcaaaataga tggtagttgt actcaatttt aaacctcata
     8761 ataaatacga aaa
//
LOCUS       DAELLV010000157         8702 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.157, whole genome shotgun sequence.
ACCESSION   DAELLV010000157 DAELLV010000000
VERSION     DAELLV010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8702
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.157"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            96..1316
                     /locus_tag="KPD58_002683"
     CDS             96..1316
                     /locus_tag="KPD58_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0266436.1"
                     /translation="MQQALKLKYQTKELGKDFVIESCNSIKPWLINIRRELHKIPELA
                     LEENLTKQKVISYLKEIGIDYMEFTKHNGIMAYILKESADKTICIRADMDALPIEEEN
                     NIPYKSIHSGKMHACGHDAHTTMLLGACKVLHSIKDKLNVNVKFLFQPAEEGFGGAKF
                     LVEDGCLENPKVDYIFGLHVMPHIETGFIETKYDTLNASVDTIKISIKGKRAHGAYPE
                     NGIDAIVTASQIVTSLQTIISRNLEPNNAAVLTIGKIYGGDAHNVICEDVKLEGTLRT
                     LNSKTRNFMIDKIAKIVGDTASAFGCVGTLHVSDENYPAVINEKELVDTVISNTKELL
                     GEEKFILRPNPSLGGEDFSFYTEHCKGAFFHLGCKNEEKGLISPLHTSSFNIDEDCLP
                     IGVMMHVMNTLYFN"
     gene            1353..2702
                     /locus_tag="KPD58_002684"
     CDS             1353..2702
                     /locus_tag="KPD58_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG0266437.1"
                     /translation="MEKAKKLGLFSMILLGINSIIGSGIFLLPGKVYNLAGQNSMFIY
                     IFATLLVLSILLCFAEVGSMFDKNGGAYLYSKKAFGDFIGFEVGTMSWVIRIISWSTL
                     AVGFATALGSFWPESATEYKGYIAAILVTLLSINSLFGIKSTKIMNNVITIAKLVPLI
                     VFIIVGIFFIKFVNIVPSGNVVNSSMGPAIILVFYAFTGFESFIVASGEMENPKKNLP
                     VALITTIFICAIIYILIQIVCMGILGDRLFENSIPIADASSVFLGNYGKVFISVATLI
                     SIFGINIGSSIVTPKCGSSLAEEGSLPAFIGKTNKYGAPYVAIIISLICCIPLVLTGS
                     FEQLAVMSVIARFAQYIPTCLSVIVLRKRTDVKASFKIPFGPVIPMVAILGSLWLLQQ
                     AWAEDISKPITQNRVLIGLGAMLLIAPLYIFMKKNKEIEHKNKSEDSKLYISREKIQ"
     gene            2816..3378
                     /locus_tag="KPD58_002685"
                     /pseudo
     CDS             2816..3378
                     /locus_tag="KPD58_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3486..5819)
                     /locus_tag="KPD58_002686"
     CDS             complement(3486..5819)
                     /locus_tag="KPD58_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG0266438.1"
                     /translation="MRSYLELVPQYAKVHKKKNRTTILCIVIAVCLVTAIFGLADMAI
                     QCQKVQFIKDDGNWHIVLKNLNEEAISEISSRVDIAHYGRGQVIDNGEFKSKEILIQG
                     LDEDLAKQLGLEIIKGRYPVVENETLIDVDAVKSFNLKVGDFIKIKLADGRNKNYKIV
                     GEYSSLSSLRASDKHGLMLSILGTQAIKKSETYDYYYITFKDKVNIRKATSDIKNTFN
                     LSDKQIGKNEKLLSIIGQGDSDYSKPLYLTAGGLFILVLIASIIMIYNSFNMGVIERI
                     KFFGLLRCLGASKLQVKKFVVLESLILSVKAIPLGLLLGCIVTIISSIFLKYVNTELF
                     GSMPILKVSFIGIVFGIIVGFLTVILSAIVPAKKASGVSPLSAIRGNLMFKEILTSGK
                     ASKIANAGSSKIDVSMGINHAFSRKKSFILMTSSFAISIVLFLGFSVFIDFMYQALKP
                     IKPEAAHISIVDADFKSVLTKDDINEISNIKGIKKVYSRMMTDVEATYNNKEATSQLI
                     SYEKNQFDWAKKYLVKGEINEDKLESENIGLVEEGHGFNIGDKIYIKNGNSKKEIEVA
                     GILSSISFDVKDKYIGNIITSENTFTSITDIKDYIIIDTQVDEDAPEDLIQNIRNVLD
                     DNLEVRDKRQGNLEAKNSFYTMAVFVYGFVFIIAIISMFNIINSMNISVTSRINYYGI
                     MRAIGMSNKQLRKMVIVESSTYAVSGCLLGSVLGLILHRYIFVSLVTLKFHIDWQIPF
                     DLLFIIVVTMIIITLLAVRKPIKKICELDIIEVVNAQ"
     gene            complement(5816..6499)
                     /locus_tag="KPD58_002687"
     CDS             complement(5816..6499)
                     /locus_tag="KPD58_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0266439.1"
                     /translation="MDILVVNNLCKTYGNGYTKVEALKDVSFTVSKGEFVTVVGESGS
                     GKSTLLNLLGGLDTPTSGKIFVDGKEICSMKEEKQTIFRRRNIGFIFQSYNLIPELTV
                     EQNIIFPLLLDYKKPDTIYLDEVLTILGLKDRKSHLPNQLSGGQQQRVAIGRALITKP
                     SIILADEPTGNLDSKNSSDVISLLKVTAEKYNQTIIMITHNTNIASTADRILQVSDGM
                     LKDLGVNRE"
     gene            complement(6570..7811)
                     /locus_tag="KPD58_002688"
     CDS             complement(6570..7811)
                     /locus_tag="KPD58_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0266440.1"
                     /translation="MKVFSNKDIKIFFLVIISVLLMFIVVGQIVVINITNDYKSALLE
                     HDYNIAGYLNSMGVDKSKIPAVFTTNEDKYTLKGKEILNTARYDFGTDDSFMPSVKLF
                     QGKYIKTMFLYLFVFFTMIIFILYVYFFKQQNKIEEASYKIDSFMNGNINVRLNTYEE
                     GSLSKLFGSIDAMSTSLNTHILKEKQNKEFLKNTISDISHQLKTPLTALMMYNQILQE
                     ESHNSEIVENFVQKSQNELERIESLIQNLLKITKIDSKTIILNKASVNVKKLITKILY
                     SFETRAEKEGKSISIRGLENTNLFCDYEWISEALSNLIKNALDNTKENDKIMIEWVET
                     PITTIISVSDTGNGIHIEDIHHIFKRFYRSKFSKSNQGLGLGLPLVKSIIEQHNGTIT
                     VESNFLQGSTFTLSFLKLTNM"
     gene            complement(7822..8508)
                     /locus_tag="KPD58_002689"
     CDS             complement(7822..8508)
                     /locus_tag="KPD58_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0266441.1"
                     /translation="MSSILLVEDDLSLIYGLEYSIQKNGFSVDVARTVEEALQIYKEK
                     NYDLLLLDVSLPDGDGFDICKRVREASNVPIIFLTASDEEVNVVMGLDMGGDDYITKP
                     FKLNELISRIKALLRRYNFTSENVTELKSNNITVKLLENRVFKNEIELELTTAEYKLL
                     CLLMKNKNIVLTRKNILDKLWDGNGSFVDDNTLSVYVRRLRNKIEDNPENPKILLTVR
                     RMGYKWNVIK"
BASE COUNT         3043 a         1335 c         1111 g         3213 t
ORIGIN      
        1 attcttataa aaaacaaagt tctccatcga aaattatggc ataataattg cttattatat
       61 taataaaaca ataaaaagca attggaggaa taaaaatgca acaggctttg aaattaaaat
      121 atcaaaccaa agaattagga aaagattttg taatagaaag ttgtaattct ataaaaccat
      181 ggcttataaa cataagaaga gaattacata aaattccaga attagctctt gaagaaaatc
      241 ttactaaaca gaaggtaatt tcttatttaa aagaaatcgg aattgattat atggaattta
      301 caaaacacaa tggaattatg gcatatattt taaaagaaag tgcagataaa acgatttgta
      361 taagagcaga tatggatgct cttcctattg aagaagaaaa caacatacct tataaatcaa
      421 tacattctgg aaaaatgcat gcttgtggcc atgatgcaca tacaaccatg ctacttggag
      481 catgtaaagt tttacattca ataaaagata aacttaatgt aaatgtaaaa ttcttatttc
      541 aaccagcaga agaaggtttt ggaggagcaa aattcttagt tgaagatgga tgtttagaaa
      601 atcctaaggt cgattatata ttcggacttc atgttatgcc acatatcgaa acaggattta
      661 tcgaaacaaa atatgataca ttaaatgcta gtgtagacac tataaaaata agtatcaaag
      721 gtaaaagagc tcatggtgca tatcctgaaa atggtattga tgcaatagtt acagcttcac
      781 aaattgttac atcattacaa actataataa gtagaaactt agaaccaaat aatgctgctg
      841 ttttgactat aggtaaaata tatggtggtg atgcacacaa tgtaatatgt gaagatgtaa
      901 aactagaagg cactttaaga accttaaata gcaaaacaag aaactttatg attgataaaa
      961 tagctaaaat tgttggagac actgcatctg catttggttg tgttgggact ttacatgtaa
     1021 gtgatgaaaa ctatcctgct gttataaatg aaaaagaatt agtagataca gttatatcaa
     1081 atactaaaga attacttgga gaggaaaaat ttatattgag accaaacccg tctttaggtg
     1141 gtgaagactt ttctttttac acagaacatt gtaaaggagc attcttccat ttaggatgta
     1201 aaaatgagga aaagggctta atttcacctc ttcatacctc tagctttaat atagatgaag
     1261 attgtttacc aataggtgtt atgatgcatg taatgaatac tttgtatttt aattagtaca
     1321 gtaattaata aaagaatagg agataaatta atatggaaaa agctaagaaa ttaggtctat
     1381 ttagtatgat attattaggg attaatagca taataggttc aggtatattc ttactaccag
     1441 gtaaagttta taatctagca ggtcaaaaca gtatgtttat atacattttt gctacactct
     1501 tagttctatc aatactttta tgttttgcag aagttggaag catgtttgat aagaatggag
     1561 gagcatatct atactccaaa aaagcttttg gagattttat aggatttgaa gttggtacaa
     1621 tgtcttgggt tattagaatt atatcatggt ctactctagc agtaggattt gcaacagctt
     1681 taggttcatt ttggccagaa tctgccactg aatacaaagg ttatatagct gcaatactcg
     1741 taacactact ttcaataaat agcttatttg gaattaaaag cacaaagata atgaataatg
     1801 taataactat agctaaatta gtaccattaa tagtttttat aatagttgga atattcttta
     1861 taaaatttgt aaatattgtt ccttcaggaa atgtagttaa ctctagtatg ggtccagcca
     1921 ttatattagt attttacgca tttaccggct ttgaatcatt tattgtggca agtggagaaa
     1981 tggaaaatcc taaaaaaaat cttccagtcg cattaataac cacaatattt atatgtgcaa
     2041 taatttatat attaattcaa attgtctgta tgggtatact tggagataga ctatttgaaa
     2101 acagtattcc aattgcagat gcttcaagtg tatttttagg caattatggt aaagttttca
     2161 tatctgtagc aacattaata tctatatttg gtataaatat aggttcatca atagtaactc
     2221 ctaaatgtgg ttcttctcta gcagaagaag gttctcttcc tgcatttata ggaaaaacaa
     2281 ataaatatgg tgctccatat gtagcaataa taatatcttt aatttgttgt ataccacttg
     2341 tgctaactgg aagttttgaa caacttgcag taatgagtgt aattgcaaga tttgctcagt
     2401 acataccaac ttgtttatca gtaatagttt taagaaaaag aacagatgtt aaggcctctt
     2461 ttaagattcc atttggtcct gttataccaa tggtagctat tttaggtagc ttatggttgt
     2521 tacaacaagc ttgggcagaa gatatttcaa aacctataac tcaaaataga gttttaatag
     2581 gattaggggc aatgttgtta atagctcctc tatatatatt tatgaaaaaa aataaggaaa
     2641 tcgagcataa aaataaatca gaagattcta aattatatat ttcgagagaa aaaattcaat
     2701 aatattttac aaactattta aaaattattt ttctaatact atagaaataa ttagaaatgg
     2761 agatgttacg tatctaaaag tagttgataa cacggatatt ggtactttta tagatatggg
     2821 acttacaaaa gacatactag ttccttttaa agctaagact tatccattgt ttagagatga
     2881 aaaatattta ttctatatat acttggataa aagcggaaga atagctgcta ctacagacat
     2941 agattcatat ttagaaacag accatactta caatgtagga gatgttgtaa atggagttgt
     3001 atatggattc caaaccaaca actctgctat gatatgtgtt gacaataaat atgctggtgt
     3061 aatccttcat aatgaatact ttacagaatt aaaagctggg gatgttcttg aaaatttaca
     3121 tgttataaaa atatacgatg atggtaaatt aggactttct cctagaggaa acagaaaaga
     3181 tgaacttgat accttagaaa ataagatttt aagctattta gaaggttcag atggatatat
     3241 gagattcaat gataaatctg atcctaaaga tatttctatc ttatttaatt caagtaagaa
     3301 aattttaaac gtgctcttgg agtacttatg aagaaagact taatttatca agatgaagat
     3361 ggaacttatc taaagtaaat tatataacac aaaaatcgaa gtaaattatg aaagatattt
     3421 tctatttcat atttacttcg attttttatt taaattacta cctctattta aattactact
     3481 tatatttatt gtgcatttac tacctctatt atgtctaatt cacaaatctt tttaatgggt
     3541 tttcttactg caagcaaagt tattattatc atagttacta caatgataaa cagtaaatca
     3601 aaaggtattt gccagtctat atggaatttt aatgttacta aggatacaaa tatatatcta
     3661 tgtaatatta atccaagtac actccccaat aaacagccac ttacagcata tgtacttgat
     3721 tctactatta ccatttttct taattgctta ttggacatgc ctattgcacg cataatacca
     3781 tagtaattta ttctacttgt aacgcttata ttcatactgt ttatgatatt aaacatactt
     3841 ataatcgcaa ttataaatac aaacccatat acaaaaactg ccatagtgta aaatgaattt
     3901 ttagcctcta agttaccttg tcgcttatct cgaacctcta aattatcatc tagaacattt
     3961 cttatatttt ggattaaatc ttcaggagca tcttcatcaa cttgagtatc aattattata
     4021 tagtctttta tatctgttat actagtaaaa gtattttcag atgttataat atttccaata
     4081 tatttgtctt taacatcaaa tgaaattgaa gaaagtatac ctgctacctc tatctctttc
     4141 ttagaatttc catttttaat atatatctta tctccaatat taaacccatg accttcttct
     4201 accaatccga tattttctga ctctaattta tcttcattga tttccccttt tactagatac
     4261 tttttggccc agtcaaattg atttttttca taagaaatta gctgagatgt tgcctcctta
     4321 ttattgtatg tagcctctac atccgtcatc attctactgt atactttttt tattcctttg
     4381 atattactta tttcattaat atcgtcttta gtaagaacag acttaaagtc agcgtcaaca
     4441 atagatatat gagcagcttc tggttttata ggtttaagtg cctgatacat aaagtctata
     4501 aatacactaa aacctaaaaa caatacaata ctaatagcaa atgaagaagt cataagtata
     4561 aaagattttt ttcttgaaaa ggcatgattt attcccatag aaacatcaat tttagaactt
     4621 cctgcgttag caattttact tgcttttcca ctagttaaaa tttctttgaa cattagatta
     4681 ccacgtatag cacttagtgg agacacccca gaagcttttt ttgctggaac aatagctgat
     4741 aatattactg ttaaaaatcc tactattatt ccaaacacaa taccaataaa gctaaccttt
     4801 aaaattggca tcgaaccaaa taactctgta ttcacatatt tgagaaatat agaggatata
     4861 atggttacaa tacatcctaa taacaatcct agtggaatag cctttacact tagaattaaa
     4921 ctttctaaaa caacaaattt tttgacttgg agttttgatg ctcccaagca acgtaatagt
     4981 ccaaaaaact ttattctctc tattacaccc atattaaagc tattatatat catgataata
     5041 cttgctatta acacaagtat aaaaagtcct cctgctgtta agtaaagtgg tttagaataa
     5101 tcagagtcac cttgacctat aatagaaagt aatttttcat ttttaccgat ttgtttatca
     5161 cttaaattga aggtattctt aatatcagaa gtagcttttc taatattaac cttgtcttta
     5221 aatgtaatat aataataatc atatgtttct gattttttga tagcttgtgt acctaatatt
     5281 gaaagcatta atccatgctt atctgaggct cttaagcttg ataatgatga atattcacct
     5341 actatcttat aatttttatt tctaccatct gctaatttta ttttgataaa atctcctact
     5401 tttagattaa agcttttaac tgcatctaca tctattaaag tctcattctc cacaacagga
     5461 taacgacctt ttattatttc tagacctaat tgttttgcta aatcttcatc taaaccttgt
     5521 atcaaaatct cctttgattt aaactctcca ttatcaatga cttgtcctct accatagtga
     5581 gctatatcta ctctactact tatttctgat atagcttctt cattaagatt tttaagtact
     5641 atgtgccaat ttccatcatc ttttataaat tgtacttttt gacattgtat agccatgtct
     5701 gctagtccaa aaatagcagt aactaaacat actgcaatga caatacataa aattgtagtt
     5761 ctattttttt tcttatgtac ctttgcatat tgtggtacta gttctaaata gcttctcatt
     5821 ctctattcac ccctaggtct ttaagcattc catcagaaac ctgtagtatc ctatctgctg
     5881 tagaagctat atttgtatta tgagttatca taataatagt ttggttatat ttttctgctg
     5941 tcacttttaa aagtgaaatt acatcactgc tatttttact gtcaagattt cctgttggct
     6001 catctgccaa tataattgat ggctttgtaa tcaatgctct tccaattgca accctttgtt
     6061 gctgaccacc agataattga tttggaagat gactttttct atctttaaga cctaaaattg
     6121 tcaatacttc atctagataa atagtgtctg gttttttgta atctagtagt aaaggaaata
     6181 ttatattttg ttccacagtt aattcgggta taagattata tgattgaaag ataaaaccta
     6241 tattacgacg tcgaaaaata gtctgttttt cttctttcat tgaacatatt tctttaccat
     6301 caacaaatat ttttcctgag gttggagtat caagacctcc taaaagattt aataatgtac
     6361 tcttacctga gccagattct ccaactacag taacaaactc tccctttgaa actgtaaatg
     6421 atacatcttt taatgcctct acttttgtat atccatttcc atatgtttta cataaattat
     6481 taaccacaag aatatccatt tgctctatct cctttctgat gattaatata agaataattc
     6541 atacacctta ctgtaagatg tatcctatct tacatattag taagctttaa aaaacttaga
     6601 gtaaatgtac ttccttgtaa aaaattactt tctacagtta tggttccatt gtgctgttct
     6661 atgatagatt taactagtgg caaccctaat ccaaggcctt gattactttt tgaaaactta
     6721 ctcctataaa atcttttaaa tatatgatgt atatcttcaa tatgaattcc atttccggta
     6781 tcactcactg aaataatagt tgttataggt gtttcaaccc attctatcat tattttatcg
     6841 ttctcttttg tattatctaa tgcatttttt ataaggttgc ttaaagcttc tgatatccat
     6901 tcataatcgc aaaacaagtt tgtattttct agacctctta tagagatact ctttccttct
     6961 ttttctgctc tagtttcaaa actatataaa attttagtaa tcaacttttt tacgttaaca
     7021 ctagctttgt taagaattat tgtctttgaa tctatctttg taatctttaa caagttttgt
     7081 atcaatgatt ctatacgctc taattcattt tgactcttct gcacaaaatt ctcaacaatt
     7141 tcactattat gactctcttc ctgcaatatt tgattataca tcatcaatgc tgtaagtgga
     7201 gtttttagtt gatgtgatat atctgaaatt gtatttttaa gaaattcttt attctgcttt
     7261 tctttcaata tatgtgtgtt tagagatgtt gacatagcat ctattgaacc aaataacttt
     7321 gataaactac cttcttcata agtatttaac cgtacattta tatttccatt cataaatgaa
     7381 tcaattttat aactagcttc ttctatcttg ttctgttgct taaaaaaata cacatacaat
     7441 ataaaaataa tcatagtaaa gaacacaaat aaatacaaaa acatagtttt tatatattta
     7501 ccttgaaaca atttcacact tggcataaaa ctatcatctg taccaaagtc atatctagct
     7561 gtatttaaga tttcttttcc ttttaaagta tatttatctt cattagtagt aaatactgct
     7621 ggtatttttg atttatcaac acccatacta tttaagtatc cagctatatt ataatcatgt
     7681 tctaacagtg cacttttata atcattagtt atatttataa ctactatttg acctactact
     7741 ataaacatca ataaaacgga tataatcacc aaaaaaaata tttttatgtc tttattagaa
     7801 aataccttca tattaacacc ctcactttat aacattccat ttgtaaccca ttcttctaac
     7861 tgttaataga atttttggat tttcaggatt atcttcaatc ttatttctta accttcttac
     7921 atatactgac agagtattat catctacaaa actaccattt ccatcccata acttatccaa
     7981 tatatttttt cttgtaagta caatattttt atttttcata agtaagcata ataatttata
     8041 ttctgctgta gtaagctcaa gctcaatttc atttttaaaa acacgatttt caagtaattt
     8101 aacagtaata ttatttgatt ttagttcagt tacattttca cttgtaaaat tataacgtct
     8161 aagtaaagct tttattcttg aaattaactc atttaattta aaaggttttg ttatgtaatc
     8221 atctccaccc atatcaagtc ccatcactac atttacttct tcatcagaag cagttaaaaa
     8281 tatgattggt acattagaag cttctcttac ccttttacaa atatcaaaac catcaccatc
     8341 aggtaaggat acatccaaaa gcagtaaatc ataatttttt tctttataaa tttgcaatgc
     8401 ttcttctact gttcttgcta catcaacact aaatccattc ttttgtatag aatattctaa
     8461 tccatatatc aaacttaaat catcttcaac caacaatata ctactcatct caatcacttc
     8521 cattttttta ttttttactt tatatatatt atatacctaa cttattatat ttttactctt
     8581 ttaaaaatac aataccttga aatcaatgat ttaaatattt taaaattatt tttatatgat
     8641 tttatatagt taatttaata ctttgaaatc ttaataataa aaaaagctta aaatacacta
     8701 ag
//
LOCUS       DAELLV010000158         8479 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.158, whole genome shotgun sequence.
ACCESSION   DAELLV010000158 DAELLV010000000
VERSION     DAELLV010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8479
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.158"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            6..2183
                     /locus_tag="KPD58_002690"
     CDS             6..2183
                     /locus_tag="KPD58_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG0266442.1"
                     /translation="MYNKYNNLYLLLGKENVNMDSYRQLKAKIPCKETGIEIKRTLCD
                     ICSPGVHCGIDAYVKDGKVIKIEGTKEHPMNKGLLCTKGLCNREYIYREDRIKTPLKR
                     IGKRGEGKFKPISWDEAYREIAEKLNRVKSEYGAKHVAFFTGYGKWYRPFLQRFASSF
                     GSPNYGTESSSCSTSAQMAGNVATGRNFTMQDMENAKTYLGWGFNPYYSKFLNSVNLI
                     KLKEKGLKFIIVDPRITPTTQKLADLHLRIKPGTDGALALGMANLFIQKNLIDREYIE
                     KYVHGFNEYKEYVKLFDLDTVSRITGVSKDNIVKAVEMLSQGPFCINESAAPIVHHQN
                     GFQNYRAMMSLSAITGNYDRIGGNIPKQDYFAGKIGGFQTLEKEFVKEVLPKNINERI
                     GGERFPLWNEMVTQFQAMDLPRQIEEKTPYEIRALFCHGINARMFPNSGRLFKALNSV
                     DFFVNIDLFMTDTSKYADILLPACTSFEREECKSYPGGYITYTKKVIDKLYDSKSDVE
                     ILSDLANAMNIDDDLLKAGYEASIRHMFKNTCVDVDKLKESDLPLKCKDFKPYIVGSY
                     TREGYDTSTSRFELKSTIIEKYKDFGLDALPTYRGFNDNIDDEYTHLLTSGARISNAL
                     HSRLHDIKLSRILKNEASCDISSLDAKELGVISGDYIKITTKIGSLDVKVNVTDRVLY
                     KNIHMYHGYREADINSIICDNFDPYSGFPAYRSCKCKIEKMNR"
     gene            2197..2619
                     /locus_tag="KPD58_002691"
     CDS             2197..2619
                     /locus_tag="KPD58_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG0266443.1"
                     /translation="MKSLFKFNKELCTACGACLLACIDQNDNNIDNGELFRHVFTIEE
                     GENIEYFSLACIHCNEPKCLGVCPKNCFKKEEGFVVLDNQNCIGCKLCEKACEYGALI
                     YGNDGKANKCNGCIERYKCGMDSPCVMVCPTGALKFNK"
     gene            3027..3830
                     /locus_tag="KPD58_002692"
     CDS             3027..3830
                     /locus_tag="KPD58_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBG0266444.1"
                     /translation="MKKLKLIIMFALVSALLVTGCSSGNNKNKEEQKVIRVATSGTYY
                     PFAFKDKDKLKGFEVDFWDEFAKRTNCKVEWVLTDFSGLFGLLEADKADVVSAQLTAT
                     PEREKKYAFSDAYNYSGTNIIVREDDTSIKSIDDLVGKKVGVGTGAVANEILKEKYPN
                     DEIKIVNYSSATLKGNYQDLEMGRLDAVVAQDVEALIAIKEQKLNLKMIEPPIQFGAC
                     SFALQKNEKGEKWTKEINEVIEEMYKDGTLTKISENWLGKDITKEPINE"
     gene            3823..4491
                     /locus_tag="KPD58_002693"
     CDS             3823..4491
                     /locus_tag="KPD58_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBG0266445.1"
                     /translation="MSNFNFEFCLSIFPFLIKALGITILISIVSFFLSILLGVILAFF
                     TQTKSKVLQYITRVYVSFFRGTPLLIQIFFFYFGFPMIFSFMKDCSPYLAIVICLGVN
                     SSAYMAEIIRASIESVDKGQQEACYAFGLSKYQSMVKIILPQAATSAVPPIVSCTLDI
                     IKGSSLGLTIGIQDLMGVAQVEAASSFKYFEIYMITILIYWAVSIILSNIQSKVEKRI
                     RRAY"
     gene            4727..5389
                     /locus_tag="KPD58_002694"
     CDS             4727..5389
                     /locus_tag="KPD58_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBG0266446.1"
                     /translation="MAFNMKYFLELFPTIMKSFNVTVILAISSLVFSLIIGTIVALSS
                     YYKVKVLNQICKVYVSIFRGTPLMPQLFFLYFGLAYMSDFVKNMDPVFATSIVLSLNM
                     GAYMSETIRASILSIDTGQIEAAYAMGLTNLQTMKRIVIPQAVRVALPSLFNNFIDLI
                     KGSSVAFVVGVNDIMGAAKSQGALSFKFFEVYGAVMVIYWCIITPLGWGQKKLEKKIS
                     NY"
     gene            5449..6246
                     /locus_tag="KPD58_002695"
     CDS             5449..6246
                     /locus_tag="KPD58_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG0266447.1"
                     /translation="MIKRNIIKCLVAVLSLTFVITGCSKKEEVKNEKVITVASSNSAD
                     PYSYVDNGEHKGFEVDMWKEIGKRSGYKIKMEPTGFSSIFGMLDSGKVDVAANFFGMS
                     KERLEKYDASIPYGSDSVCIAVKDGNNEINKLEDLKGKVVAVSEGSQGQQVATSVNKD
                     NLFQEKVYGDGTSGLQDLDLGRVSAWIDAELTIVGDAKKANMKVRVLDKKLSATNIAY
                     FFKKNDDKSKTIKEDVNKAIEEMLADGTVKKISEKWFGMDVSADIQK"
     gene            6364..7554
                     /locus_tag="KPD58_002696"
     CDS             6364..7554
                     /locus_tag="KPD58_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0266448.1"
                     /translation="MEVLQMIETKIKSIALKNREKIIKIRRQIHSNPELAFKEYKTSK
                     LIKEELNKLNIEYINIAGTGVLATIKGKNNGGKTILLRADMDALPIKEENDLEFKSIN
                     DNMHACGHDAHVSWLLGTAMILNDIKEELNGNVKLLFQPGEEKGGSDIVIKENVLEGV
                     DALATGHCWPTIESGKIGIARNCAMAATNTFEITIIGKGGHGAEPHNCIDPIAVGNAV
                     YSSIQQIVSRKIDPVVPVVVSVCSFNSGVSKNIIPDVCTLQGTIRAISKEQVIEVSKI
                     LEYIVKNVCKAHGADCKFEKSTGGDAVINDKNMIEIGRESACKILGAENVEIIDFPAM
                     TGEDFAIYMKEKPGLFMYIGVGDKEKNINYRLHSNKFNIDEKCLSIAPSLFSQLTVDY
                     LCQL"
     gene            7663..8412
                     /locus_tag="KPD58_002697"
     CDS             7663..8412
                     /locus_tag="KPD58_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBG0266449.1"
                     /translation="MIKVENLHKSFKGTEVLKGINLEISQSEVVAILGSSGTGKSTLL
                     RCLNYLVEPTKGKIMIGDVKVDVEKATKKDITNLRKHTSMVFQNYNLFKNKTALENVM
                     EALIVVHKKSKKEAREISLKLIDKVGMLDRKDFYPSKLSGGQQQRIGIARAMAVNPNV
                     ILFDEPTSALDPELVGEVLSVIKSLAKEGITMLIVTHEINFAKEVADKIIFMDNGVIA
                     EEGTPDEIFNNPKNERTAKFLNIIRKEEVFS"
BASE COUNT         3359 a          854 c         1576 g         2690 t
ORIGIN      
        1 aaagaatgta taataagtat aataatttgt atttattatt gggaaaggaa aatgttaata
       61 tggatagtta tagacaactt aaagctaaaa taccctgtaa ggaaacaggt atagaaataa
      121 agaggacttt atgtgatatt tgttctccag gagtacattg tggaattgat gcatatgtta
      181 aagatggtaa agtaataaaa atagaaggta caaaagaaca tccaatgaat aaagggttgt
      241 tatgtaccaa aggtctttgc aatagagaat atatctatag agaagataga ataaaaacac
      301 ctctaaaaag aattggaaaa agaggtgaag gaaaatttaa accaatatca tgggatgaag
      361 cttatagaga aatagcagaa aaattaaata gagtaaaatc tgagtatgga gcaaagcatg
      421 tagctttttt tacgggttat ggaaagtggt atagaccttt tttacaaagg tttgcatctt
      481 catttggttc acctaattat ggtacagaat ctagttcatg ttcaacatca gcacagatgg
      541 ctggaaatgt tgctactgga agaaacttta caatgcaaga tatggagaat gcaaaaactt
      601 atttagggtg gggatttaat ccatactact ctaaattttt aaatagtgtg aatctaataa
      661 aattaaaaga aaaaggtcta aaatttataa ttgtagaccc tagaataaca ccgacaactc
      721 aaaaactagc agatttacat ttaagaatta aacctggaac tgatggagct ttagctcttg
      781 gaatggcgaa tctatttata caaaagaatt taatagatag agaatacata gaaaagtatg
      841 tacatggatt taatgaatat aaagaatatg taaaattatt tgatttagat actgtaagta
      901 gaattacagg agtttctaaa gataatatag taaaagctgt tgagatgttg tcacaaggtc
      961 cattttgtat aaatgaaagt gcagctccaa tagttcacca tcaaaatgga tttcaaaatt
     1021 atagagcaat gatgtcttta tctgctataa caggtaatta tgatagaata ggtggaaaca
     1081 taccaaaaca agattacttt gctggtaaga taggtggatt ccaaacacta gaaaaagagt
     1141 ttgtaaaaga agttctacct aaaaatataa atgaaagaat tggtggagaa agatttcctc
     1201 tatggaatga aatggtgact caatttcaag ctatggattt acctaggcaa atagaagaaa
     1261 agacaccata tgaaataaga gctttgtttt gtcatgggat aaatgctaga atgtttccta
     1321 attcagggag actttttaaa gcgttgaata gtgtagattt ttttgtgaat atagatttat
     1381 ttatgacaga tacatcaaaa tatgcagata tacttttacc tgcatgtaca tcatttgaga
     1441 gagaagaatg taagagttat ccaggagggt atattacata taccaaaaaa gttattgata
     1501 aattatacga ttcaaaatca gatgtagaaa tactttcaga tttagcaaat gctatgaata
     1561 tagatgatga tttattaaaa gcaggttatg aagcaagtat aagacatatg tttaaaaata
     1621 catgtgtaga tgtagataaa cttaaagaaa gtgatttacc attaaaatgt aaagatttta
     1681 agccatatat agtaggttca tatacaagag aagggtatga tactagcact tctaggtttg
     1741 aattaaaatc tacgattatt gaaaaatata aagattttgg actggatgca ctaccaacat
     1801 atagaggatt taatgataat atagatgatg aatatactca tttattgaca tcaggagcaa
     1861 gaatttcaaa tgcactacat tcaagacttc atgacatcaa actttcaaga atattaaaaa
     1921 atgaagctag ttgtgatatt tctagcttag atgcaaaaga attgggtgtt atcagtggag
     1981 attatataaa aataactact aagataggaa gtttggatgt aaaagtcaat gttactgata
     2041 gagtcttata taagaatata catatgtatc atggatatag agaagctgat ataaatagta
     2101 ttatttgtga taattttgac ccttatagtg gattccctgc gtatagaagt tgtaagtgta
     2161 agattgaaaa aatgaataga taaatggaga gatataatga aaagtttatt taaatttaat
     2221 aaagaattat gtacagcatg tggagcatgt ttgcttgcat gtatagacca aaatgataat
     2281 aatatagaca atggtgaact ttttagacat gtatttacaa tagaggaagg tgaaaatata
     2341 gaatattttt cattagcatg tatacattgt aatgagccaa aatgtttagg tgtatgtcct
     2401 aaaaattgtt ttaaaaaaga agaaggattc gttgtattag acaatcaaaa ctgtataggg
     2461 tgtaaacttt gtgaaaaagc ttgtgaatat ggagccttaa tttatggaaa tgatggtaag
     2521 gcaaataagt gtaatggttg tattgagaga tataagtgtg gtatggattc accttgtgta
     2581 atggtatgtc ctacaggagc attaaaattt aataaataaa ttaaatatag agaatgactt
     2641 atacttaggt ataagatatt tttacaaaaa gctgtattga gtttgttata atgaaaactc
     2701 aatatagctt ttttatggat atagttggat actataatgt gattcttagt aatagttttt
     2761 tgctattgga caaagactgt aaaacatatt aaacttatgt tgttcaaagt cgaagtataa
     2821 tataataact agaaaattgg tttatagtgc tataaaagca aatttagtaa ggtataaaat
     2881 cagaggcaat cctttactat aatatcaata tttgggagtt ctgttattgc agagctatta
     2941 aacaattata atggtacaag tgattcaacc tttgaaaatg tattatataa aaaattaaaa
     3001 taagattaaa aaggagagaa taaattatga aaaaacttaa attaattatt atgtttgcct
     3061 tggtatcagc actgttagtt acaggatgtt catcaggaaa taataaaaat aaagaagaac
     3121 aaaaagttat aagagttgca acatcaggaa catattaccc ttttgctttt aaagataaag
     3181 ataaacttaa agggtttgaa gtagattttt gggatgaatt tgctaaacga actaattgta
     3241 aagttgaatg ggtattaact gattttagtg gattatttgg attattagaa gctgataaag
     3301 cagatgttgt atctgctcaa cttactgcaa cacctgaaag agagaaaaaa tatgcttttt
     3361 cagatgctta taattatagt ggaacaaaca ttattgttag agaagatgat actagtataa
     3421 aatcaataga tgatttagtt ggcaagaaag taggtgtggg tactggagca gtagcaaatg
     3481 aaatattaaa agaaaaatac ccaaatgatg aaataaaaat agtaaattat tcaagtgcga
     3541 ctcttaaagg gaattatcaa gacctagaaa tgggaagatt agatgcagta gttgcgcagg
     3601 atgttgaggc tttaatagct ataaaagaac aaaaattaaa tcttaaaatg atagaacctc
     3661 caattcaatt tggtgcctgt tcttttgcgc ttcaaaaaaa tgaaaaaggt gaaaaatgga
     3721 caaaagaaat taacgaagta attgaagaga tgtataaaga tgggactttg actaaaatat
     3781 ctgaaaactg gctaggaaaa gacattacta aggagcctat taatgagtaa ttttaatttt
     3841 gaattttgtc taagtatatt tccatttttg attaaagcat tgggaataac aatactaata
     3901 tctattgttt catttttttt atcaatatta ttaggtgtaa tacttgcctt tttcacacag
     3961 acaaaatcaa aagttttaca atacataaca agagtgtatg tttcattctt tagaggaaca
     4021 ccacttttaa ttcaaatatt tttcttttat tttggatttc caatgatatt ttcttttatg
     4081 aaagattgtt caccttattt agcaatagta atttgtttag gtgtaaattc atctgcatat
     4141 atggctgaaa ttatacgagc atctattgag agtgttgata aaggacaaca ggaagcatgt
     4201 tatgcttttg ggttaagtaa atatcagtca atggttaaaa taattctacc tcaagcagct
     4261 acatctgcag tacctcctat tgtaagctgt acactggaca taattaaagg ttcttcttta
     4321 ggtttaacta taggaataca agatttaatg ggagtagctc aagtagaggc tgcttcaagt
     4381 tttaaatatt ttgagatata tatgataacg atattaattt actgggcagt atctataata
     4441 ttgagtaata tacaaagcaa agttgaaaaa agaataagaa gagcatatta gatttcaata
     4501 tttaaaaatg ggatgatata gatatattat tacttgtaaa taaattttta attttagaaa
     4561 aataaactta gtaatctaaa ttattgagaa aatatagtat caaaatggac tgtataatca
     4621 agttaaaaaa tagtttttct attgaaaaaa taaataaaat tctattgtaa tttaatatgc
     4681 gataaagttt tataaaaaac aacattaagc tagggggact tattttatgg cttttaatat
     4741 gaaatatttt ttagaattat ttccaacaat aatgaaatcg ttcaatgtaa ctgtgatttt
     4801 ggcaataagt tcattagtat tttcattaat tataggtaca atagtagcgt tatcatctta
     4861 ttacaaagtg aaagtattga atcaaatatg taaagtatat gtatctattt ttagaggtac
     4921 acctcttatg ccacagttat tctttttata ttttggacta gcatatatga gtgattttgt
     4981 taaaaatatg gacccagtat ttgcaacatc tatagtactt agtttaaata tgggagctta
     5041 tatgtctgaa acgataaggg catctatact ttcaattgat acaggtcaga tagaagcagc
     5101 atatgcaatg ggattaacaa atttacaaac aatgaaaagg atagtgatac cacaggccgt
     5161 aagagttgca ctgccatcat tattcaataa ttttatagat ttaattaaag ggtcttctgt
     5221 agcttttgtt gttggagtaa atgatataat gggtgctgcc aaatcacaag gagcgttgag
     5281 ttttaagttt tttgaagtgt atggagcagt tatggtaatt tactggtgta taataactcc
     5341 tttaggttgg gggcaaaaaa aattagaaaa gaaaataagc aattattaag ttattaagaa
     5401 aatacataaa agtattttta tataaaaaat ggtgtgagga gaagagatat gataaaaaga
     5461 aatattataa aatgtttagt ggcagtttta tcgttaacgt ttgtaattac tggttgttca
     5521 aaaaaagagg aagtaaagaa tgaaaaagtt ataactgtag cttcatctaa ttcagctgac
     5581 ccatattctt atgttgataa tggtgagcat aagggttttg aagttgacat gtggaaagag
     5641 attggtaaaa gaagtggata taagataaaa atggaaccaa caggttttag ttctatattt
     5701 ggtatgttag attctggtaa agttgatgtg gcagccaact tctttggaat gtctaaagaa
     5761 agattagaaa aatacgatgc ttctatacct tatggttctg attctgtgtg tatagctgta
     5821 aaagatggaa ataacgaaat taataagctt gaagatttaa agggaaaagt tgtagcagtt
     5881 tcagaaggtt ctcaaggtca acaggttgca actagtgtta ataaagataa tttgtttcaa
     5941 gaaaaggttt acggtgatgg aactagtggt cttcaagatt tagatttggg aagagttagt
     6001 gcatggattg atgcagagct tactattgtg ggagacgcta aaaaagctaa tatgaaagta
     6061 agagtattag ataaaaaatt atcagctact aatatagcat acttctttaa gaaaaacgat
     6121 gataaatcta aaacgataaa agaagatgta aacaaagcaa tagaagaaat gcttgcagat
     6181 ggaacagtga aaaaaatatc tgaaaaatgg tttggaatgg atgtaagtgc tgatatacaa
     6241 aaataaatac tttaaaatta aaatgtaaaa ctgtgataac tatagataag tatactaaat
     6301 ttatctatag ttatctgtgt attacaatgt atttagatta gtctttataa atcaaaatga
     6361 gttatggagg tattgcaaat gattgaaact aaaataaaat ccattgcact aaaaaataga
     6421 gaaaaaatta taaaaataag acgacagata cacagtaatc cagagttagc atttaaagaa
     6481 tataaaacat ctaaacttat aaaagaagag ctaaataaac ttaatataga atacataaat
     6541 attgctggta caggagtatt agctactata aaaggaaaaa ataatggtgg aaaaactata
     6601 ttactcagag cggatatgga tgctcttcca atcaaagagg aaaatgattt agaatttaaa
     6661 tcaataaatg ataatatgca tgcttgtggg catgatgcac atgtttcatg gttgttagga
     6721 acagcaatga ttttaaatga cataaaagaa gaattgaatg gaaatgtaaa attactattt
     6781 caacctggcg aagaaaaagg tggttctgat attgtaatta aagaaaatgt acttgaaggt
     6841 gtggatgcat tagcgactgg tcattgttgg ccaactattg agtcaggaaa aattggaatc
     6901 gctagaaatt gtgctatggc agcgactaat acttttgaaa taacaataat tggcaagggt
     6961 gggcatggag cagagcctca taattgtata gacccaattg cagttggaaa tgcagtatat
     7021 tcatccattc aacagatagt ttcaagaaaa attgacccag ttgtacctgt agtagtgtct
     7081 gtttgttctt ttaattctgg tgtttctaaa aatataatac cagatgtatg tacacttcaa
     7141 gggactatta gagcgataag caaggaacaa gtaattgaag taagtaaaat tctagaatat
     7201 atagtaaaaa atgtatgtaa agcacatgga gcagattgca aatttgaaaa gagtacagga
     7261 ggagatgctg ttataaatga caaaaacatg atagagatag gaagagaaag tgcttgcaaa
     7321 atacttggag ctgaaaatgt tgaaataatt gattttcctg caatgacagg tgaggatttt
     7381 gctatttata tgaaagaaaa acctggttta tttatgtata taggtgtagg agataaagaa
     7441 aaaaatataa attacagatt gcatagtaac aagtttaata tagatgaaaa atgtcttagt
     7501 atagcacctt cattattttc acaacttaca gtagattatt tatgtcaact atagagtaca
     7561 ttcataaaaa tatatttagc ctatttttaa atttaatgct agtatattaa ttttgatgta
     7621 gaacatatta tttattatat ttatttaaaa aggtggataa caatgataaa agtagaaaat
     7681 ttacataaaa gttttaaagg gacagaagtt cttaaaggta taaatttaga gataagtcaa
     7741 agtgaagtag tagctatact tggatcatct ggaacaggta aatctacgtt acttagatgt
     7801 ttaaattatt tagttgagcc tacaaaagga aagataatga ttggtgatgt aaaagtagat
     7861 gtagaaaaag caacaaaaaa agatataacc aatttaagaa aacatacatc tatggtgttt
     7921 caaaattata acttatttaa aaataaaaca gcattagaaa atgtaatgga ggcattgata
     7981 gtagttcata aaaagagtaa gaaagaagct agagaaatat ctttaaaatt aatagataaa
     8041 gtaggtatgt tggataggaa agatttttat ccatcaaagt tgtctggggg acagcaacaa
     8101 aggataggta tagcgagagc gatggcagta aatccaaatg taatattatt tgatgagcct
     8161 acatctgcac ttgacccaga acttgttgga gaagttttaa gcgttataaa atctcttgca
     8221 aaagaaggta taacgatgct tattgtaact cacgaaataa attttgcaaa agaagttgct
     8281 gataaaatta tatttatgga taatggtgta atagctgaag aaggaactcc tgatgaaata
     8341 tttaataatc ctaaaaatga aagaacagca aaatttttaa atatcattag gaaagaagaa
     8401 gtatttagtt aaaagtgtat aaattaagca ttagaagata tgtaagatac ccccattaaa
     8461 tttataagtt agataataa
//
LOCUS       DAELLV010000159         8418 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.159, whole genome shotgun sequence.
ACCESSION   DAELLV010000159 DAELLV010000000
VERSION     DAELLV010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8418
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.159"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            156..608
                     /locus_tag="KPD58_002698"
     CDS             156..608
                     /locus_tag="KPD58_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0266450.1"
                     /translation="MVLQEKYIFIDKDLKSKEEVLNFIADSAYILGITTNKEALLKDL
                     LKRENEFPTGIKDGFAIPHTKSVNVKEASILYIKTTNELEWKTLDDSKVRYVFSLLAP
                     HKNEGNIHLKTLSKLATCLMEDDFTSEVKNASDASHLVKYITNKMKEE"
     gene            611..919
                     /locus_tag="KPD58_002699"
     CDS             611..919
                     /locus_tag="KPD58_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBG0266451.1"
                     /translation="MKIVAITSCIAGLAHTPMAAKALEQAASKMGHEIKVEQQGAMGR
                     VNTITKEEAQDADFILIASDQTISGMERFDGKKVIKVKIGIALKKPEAVLTKCIEAIS
                     "
     gene            1033..2139
                     /locus_tag="KPD58_002700"
     CDS             1033..2139
                     /locus_tag="KPD58_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBG0266452.1"
                     /translation="MSKKNKSLLSELKGHLMTGISYMLPLVIGSSLVVAIPKLTALAV
                     GITSLDAYKNVSGFYHILYLMEQVGWTGIGLLNTVLAGFIAFSIGEKPAMGAGFIGGL
                     IASNTNAGFLGAVIAGFFAGYVCKFLKKKIKISGSASGMMPLIIMPLITVGLTGFLMS
                     VVLAGPLGNINTSLNAWVAEMCQNGTNSVVLALILGAMIGFDLGGPVNKAAWMAGNAL
                     LLEGIYLPAILVNCAIVIPPLGYGLATFIRKSKFSNALAETGKGNIVMGIIGITEGAI
                     PFTLTNPLKLVPVNMIGCALGVGLSALLGVHAIMPPVGGLYGFISIGSGWAYLVGAIF
                     GALVIAILSTTLVDFNEDETTENTEFDEIELDIM"
     gene            2214..4892
                     /gene="mngB"
                     /locus_tag="KPD58_002701"
     CDS             2214..4892
                     /gene="mngB"
                     /locus_tag="KPD58_002701"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBG0266453.1"
                     /translation="MKKTKVHVVPHSHWDREWYFTTSRSKIYLMKDFQDILDILEEKE
                     DFKYFTMDAQASLLDDYLKWRPQDKYRIRDLVQRRRLVIGPWYTQTDQLVISGESIIR
                     NLYYGIDRCNEFGEPMRVGYVPDSFGQSAQMPQIYRGFGIDSSLFWRGVSDDMVDTTE
                     FIWKGSDGSKVLAVQIPFGYYYGGNIPEDDSDLKEYLKDIIVKLKVKASTNNVYFPNG
                     FDQAPIRKNLPDILKKANEIDNENEYEISDIENYIDSVRNERNEFTELKGEFLNAKHM
                     RIHKSIFSSRSDLKIMNNKLENYITNVLEPLLTLSYSLGNEYPHLVIKDIWKLMFENA
                     AHDSIGSCNSDTTNEDVYTRYKQARDLSINLLDLHMRLISTQIKNNNEEITLTVFNGF
                     PEVRNEVVEFDTYIPGQDFVIKNKNGKILNYIIKEKEDITNYILTQTIRLNPSKKIYI
                     PKTVYRAKIALSVKDIPPMGYTQLVFELDTKNKCEIETSNCKEIENKYYKITANKNGS
                     LEILDKESNKTYKNQAVIEDNGDDGDSYNYSPPRKDIYVSSLDSVSSVEVLKSNIQEE
                     MILKYSLTIPASLEERAIGKVSVKMPVTMKITLEPKEQIIKFNVNIKNNQALSHRVRV
                     LFNTEIASKFSIADQQFGIIQRPTVLEKDLALWKRDNYSWQEKPITIEPMQSFVTLED
                     GQRGIALITGCVREYQIVGDNLDTIALTLFRSFGYMGRENLLYRPGRASGEKIIATPD
                     AQLLKELNFDFGLYIYNNKFDEANVANTAKRFLTPIQIYEYADFLNGRLIFAFNDEKQ
                     IYENEFSLMNVNNSNFTVSAIKKEEKGDGIVVRIFNGMKNEDAKGSLNINKNVNDAYS
                     AMLDEIYDNTSKLKVNTKTVEVNSLSHCKVQTIVIK"
     gene            complement(5209..7542)
                     /locus_tag="KPD58_002702"
     CDS             complement(5209..7542)
                     /locus_tag="KPD58_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG0266454.1"
                     /translation="MDTKLKKFKDKYIVTIIVFILMLSASLGMISQYSTIQASAKGGA
                     ENPFEQESFVDSIYKGSYVLYHNMKEEQEGKMIQPSKLFINEETMNYIKNVSKEKDTY
                     DGSETYYTDDSINADFNENFENWEHFIKASGKNLKYYLVDKESNFEFFNDNKELGVLD
                     TTKNDASKENSDENQSKNTEMNDEERQKQITDIKENYRFYVVMNFDKDGKVNIVDSYG
                     VNQKIINQMLLSKAREDLMDTSLGGNSISYKLSPIKNITLVYAVPKVISKGDSVEYGY
                     GNTYFDDGIISYISNVENNSYYKISGMILKIILAIVVIVALAFPYRKSKELLGFDTLS
                     RIPLEILFIALDIIQGLVDLYPTVIISNTLSGNYVEYLINNDISAKMASILVSLMNLT
                     YWFVIFSIIFVFIILLKHILNVGIREYFTKHTVTGMFFVWLKKAGKTVINQIRTINLK
                     EKPNKTIVTILGINLLIIVIMCSMWFFGIILALIYSVVLFKILSDYSKKTIYEYNQLL
                     DVTKKISDGNLDVNMEKDLGFFNPIKDELGNIQTGFKKAVDEEVKSQKMKTELISNVS
                     HDLKTPLTSIITYIDLLKDESITDENRKLYIDTLDRKSQRLQHLIEDLFEVSKANSGD
                     VYLNIVNVDIVSLMKQTLLELDDKLADSSLIVKNNFSNEKIILPLDSQRTFRVFENLI
                     INISKYAMPNSRVYIDILETDNKVNIMLKNMSASEIDFSVDDIMERFVRGDKSRNTEG
                     SGLGLAIAKSFIELQGGKMNISVDGDLFKVTITFNKN"
     gene            complement(7517..8233)
                     /locus_tag="KPD58_002703"
     CDS             complement(7517..8233)
                     /locus_tag="KPD58_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0266455.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIVDIMMPKMEGITLTMKLRENYNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVGNKEQKVESDDSVFAIGGLELNENTKEVSIDG
                     KYIKTTPIEFKILSLLMRNAGRVFSADEIYERVWNENAVNTDTVMVHVRNIREKIEID
                     PKNPKYLKVVWGVGYKIEKI"
BASE COUNT         3137 a         1182 c         1164 g         2935 t
ORIGIN      
        1 ttataataat ttatttccac ttctatatgg aaattagtta ttatcataat ataaatttcc
       61 atgtcgaagt ggaaattagt tatatttaat ttaattagga taatgattac aatataaata
      121 taagttaaga cattatgaat ggagtggaaa aatatatggt actacaagaa aaatatatat
      181 ttatagataa agacttaaaa agcaaagaag aagtattaaa ttttattgca gatagtgctt
      241 atatactagg tattacaact aataaagagg ctcttttaaa agaccttcta aaaagagaaa
      301 atgaatttcc aacaggaata aaagatggat ttgcgatacc tcatacaaag agtgtaaatg
      361 taaaggaagc ttccatactc tatattaaaa caactaacga gttagagtgg aaaactttag
      421 atgatagtaa ggttagatat gtatttagtt tgttagcccc acataaaaat gaaggaaata
      481 tacatttgaa aactctatct aaattagcca catgtttaat ggaggatgat tttacaagtg
      541 aagttaaaaa tgctagtgat gcaagtcatt tagtaaaata tataactaat aaaatgaagg
      601 aggaataatt atgaaaatag tagctataac atcatgtata gcaggattag cacacacacc
      661 tatggcagca aaagctttag aacaagctgc atcaaaaatg ggtcatgaaa tcaaagtaga
      721 acaacaaggt gctatgggac gagtaaatac tataacaaaa gaagaagcac aagatgcgga
      781 ctttatactt atagcatctg accaaactat atctggaatg gaaagatttg atggtaaaaa
      841 agtgataaaa gtcaaaatag gtatagcgtt aaaaaaacca gaagctgtct taacaaagtg
      901 tatagaagct atttcttaat aaaaaaatct cactctaata tagaatgaga aaaatcaaaa
      961 attaaaaaat tgtctaatgt aattataaca tattatcaaa aaatataaca aacttatata
     1021 ggaggattaa aaatgagtaa aaaaaataaa tctcttttaa gtgaacttaa gggacactta
     1081 atgacaggta tctcatatat gttgccactt gtaataggct catctttagt tgtagctatc
     1141 ccaaagctta cagctttagc agtgggtata acaagtttag atgcttataa aaatgtatct
     1201 ggtttttatc atattcttta cttaatggaa caagttggtt ggactggcat aggtctatta
     1261 aatacggtat tagcaggatt tatagcattt tcaataggtg agaaaccagc tatgggtgca
     1321 ggttttattg gtgggctcat agcaagtaat actaatgcag gattcttagg agcagtaata
     1381 gcaggtttct ttgctgggta tgtttgtaag tttttaaaga aaaaaattaa aatatctggg
     1441 tcagcatcag gaatgatgcc acttataata atgccactta taacagttgg attaactggt
     1501 tttttaatga gtgttgtatt agcaggacct ttaggaaaca taaatacttc attaaatgct
     1561 tgggtagctg aaatgtgtca aaatggaaca aattcggtgg tattggcttt aattttaggc
     1621 gcaatgatag gattcgattt aggtggacca gtaaataaag cagcttggat ggcaggaaat
     1681 gcattattgt tagagggaat ctatctacca gcgatattgg taaactgcgc aatagttata
     1741 cctccactag gatatggttt agctacattc ataagaaaat caaaattttc caatgcatta
     1801 gctgaaacag gaaaaggcaa tatagttatg ggtataattg gaataacaga aggtgcaatt
     1861 cctttcactt taacaaatcc attaaaatta gttcctgtta atatgatagg atgcgctttg
     1921 ggtgtaggat tatcagcttt gttaggtgta catgctataa tgcctccagt tggtggatta
     1981 tatggattta tatcaatagg ttctggatgg gcttacttag taggtgcaat atttggagct
     2041 ttagtaatag caatattatc aacaacttta gtagacttca atgaagatga aactacggaa
     2101 aatacagaat ttgatgaaat agagttagat ataatgtaaa ttaaaaactt aattttagat
     2161 aattgagaat tgagtgtact ctcaattctc aattaaattt tgggaggatt tttatgaaaa
     2221 agacaaaagt acatgtagtt ccacacagtc attgggatag agaatggtat tttacaacat
     2281 caaggtcaaa aatatattta atgaaagatt ttcaagatat attagacata ttggaagaaa
     2341 aagaagactt taaatacttt actatggatg cacaagcttc tctcttagat gattatttaa
     2401 aatggagacc tcaggataaa tatagaataa gagatttagt tcaaagaaga agattagtta
     2461 ttggaccttg gtatactcaa acagaccaat tggttatatc aggagaatct ataattagaa
     2521 acctttacta tggaatagat agatgcaatg aatttggtga acctatgagg gttggatatg
     2581 taccagattc ttttgggcaa tcagctcaaa tgccacaaat atacagaggt tttggaatag
     2641 atagttcatt attttggcgt ggagtttctg atgatatggt agatacaaca gagtttatat
     2701 ggaaaggttc tgatggttca aaagtattag cagttcaaat accatttggt tattactatg
     2761 gaggtaatat accagaagat gattcagatt taaaagaata tttaaaagat ataatcgtaa
     2821 aacttaaggt taaagcttct actaataacg tatattttcc aaatggattt gaccaagcac
     2881 caataagaaa aaaccttcct gatatattaa aaaaagctaa tgaaatagat aatgaaaatg
     2941 aatacgaaat aagtgatata gaaaactata tagattcagt aagaaatgaa agaaatgaat
     3001 ttacagagct taaaggtgaa tttttaaatg ctaaacatat gagaatacac aaatccatat
     3061 tctcctcaag gtcagacctt aaaataatga ataacaaatt agaaaattat attacaaatg
     3121 tacttgaacc gttattaaca ttaagttatt cacttggaaa tgaataccct cacctagtta
     3181 taaaagatat atggaaactt atgtttgaaa atgcagctca tgacagcata ggttcttgta
     3241 atagtgacac aacaaatgag gatgtataca caagatacaa acaagccaga gatttgagta
     3301 taaatttatt agatttgcat atgagactta taagtactca aatcaaaaat aataatgaag
     3361 aaataacatt aacagtattc aatggattcc cagaagtcag aaatgaagtt gtagaatttg
     3421 atacttatat tccaggtcaa gattttgtga taaagaataa aaatggaaaa attcttaatt
     3481 atataataaa agaaaaagaa gatattacaa attatatatt aactcaaaca ataagattaa
     3541 acccaagtaa aaaaatatat atacccaaaa ctgtatatag agcaaaaatt gcactttcag
     3601 taaaagatat accaccaatg ggatatactc aactagtttt tgaattggat actaaaaata
     3661 aatgtgaaat agaaacaagt aattgcaagg aaatagaaaa caaatactac aaaataacag
     3721 ccaataaaaa tggttcatta gaaatattag ataaagaatc taataagact tataaaaacc
     3781 aagctgtaat tgaagacaat ggagatgatg gagattcata caactattct cctccaagaa
     3841 aagatattta tgtttcatct ttagattctg tatcttcagt agaagtgcta aaatcaaaca
     3901 ttcaagaaga aatgatactt aaatattcat tgaccatacc agcttcactg gaagaaaggg
     3961 ctataggtaa agtttctgtg aaaatgccag taacaatgaa aatcacctta gaacctaaag
     4021 aacaaataat taagtttaat gttaatataa aaaataacca agcactatct catagagtaa
     4081 gagttttatt taatacagaa atagcatcta agttttctat agcagatcaa caatttggaa
     4141 taatacaaag accaacagta ttagaaaaag accttgcatt gtggaaaaga gacaattact
     4201 catggcaaga aaaacctata actatcgaac caatgcaaag ttttgttact ttagaggatg
     4261 gacaaagagg aatagcatta ataactggtt gtgtaagaga atatcaaata gttggtgata
     4321 atttagatac aatagcatta acactattca ggtcatttgg atatatggga agagaaaatt
     4381 tactttatag accaggaaga gcttctggag aaaaaataat agcaacacca gatgcacaat
     4441 tacttaaaga gttaaacttt gactttggtc tttacatata taacaataaa tttgatgaag
     4501 caaatgtagc taatacagct aagagattct taacacctat acaaatatat gaatatgctg
     4561 attttttaaa tggtagactt atatttgcat tcaatgacga aaaacaaata tatgaaaatg
     4621 aatttagttt gatgaatgta aataattcaa atttcacagt aagtgcaata aagaaagaag
     4681 aaaaaggaga tggaattgta gttagaatct tcaatggtat gaaaaatgag gatgctaagg
     4741 gaagtcttaa catcaataaa aatgtcaatg atgcttatag tgcaatgctc gacgaaatct
     4801 atgataatac tagcaagctt aaagtaaata ctaaaactgt agaggtaaat tctttatcac
     4861 attgtaaagt acaaacaata gtaattaaat aatataaaat agcttataca agatttcaaa
     4921 ataaattgct agtaccatgt taatagtaaa gccagatatt atgctattta taagctctgt
     4981 aacactaaat aacattttgt aaaataaaaa ggtgaacctt gtatattagg attcaccttt
     5041 tttaaataaa tcaacattct gttttaaaat aacatttata agctgttttg aaataatgct
     5101 tacaaattat tttaaaacaa tacctataaa tttttattta aagaatacac ataaatattt
     5161 ccagactctt ttatcctata gactttataa ttattaaaga agtctaatct aatttttgtt
     5221 aaaagtaatt gtaactttaa ataaatcacc atccacactt atattcatct taccaccctg
     5281 taattctata aaactttttg ctatcgctaa accaagacca gaaccttctg tgttacgtga
     5341 tttatctcct cttacaaatc tctccattat atcatctaca ctaaaatcaa tctcagatgc
     5401 agacatattt ttaagcataa tatttacttt attatctgtt tctaaaatat caatatacac
     5461 tcttgaattt ggcattgcat atttgcttat atttattata agattttcaa atactctaaa
     5521 tgtacgctga ctatctaatg gtaatattat tttttcatta gaaaagttat tctttactat
     5581 tagagatgaa tctgcaagtt tgtcatctaa ttctaaaagt gtctgcttca ttagagatac
     5641 aatatccaca tttactatat tcaaataaac atctccacta tttgccttgc ttacttcaaa
     5701 caaatcttct attagatgtt gcaatctttg tgactttctg tctaatgtat caatatacaa
     5761 cttacgattt tcatctgtta tactttcatc ttttaataaa tctatataag tgattataga
     5821 tgttagaggt gtttttaagt catgtgatac atttgatata agctctgttt tcatcttttg
     5881 actcttaacc tcttcgtcaa cagctttttt aaatccagtt tgaatatttc ctaattcgtc
     5941 ttttatagga ttaaaaaatc ctaaatcttt ttccatattt acatctaagt ttccatctga
     6001 aattttcttt gtaacatcta aaagttgatt atattcatat attgtctttt tagagtaatc
     6061 tgacagtatt ttaaataata ctactgaata tattaatgcg agtattattc caaagaacca
     6121 catactacac attataacta taatcaataa atttattccc aaaattgtaa ctatagtttt
     6181 atttggcttt tcttttagat ttattgttct aatttgatta ataactgttt ttcctgcttt
     6241 ttttagccat acaaaaaaca ttcctgtcac agtatgtttt gtaaaatact ctcttattcc
     6301 aacatttaaa atatgtttta aaagtattat aaatacaaat attattgaga atattacaaa
     6361 ccaataagtt aaattcatta aacttaccaa tatactagcc attttagcac ttatgtcatt
     6421 gtttataagg tactctacat aattcccact caaggtatta cttattataa cagtaggata
     6481 taaatctaca agcccctgta taatatcaag agcaataaag agtatctcta acggtattct
     6541 tgataatgta tcaaatccta gcaactcttt agattttcta tatgggaatg ctaaagctac
     6601 aataacaact atagctaata taatttttaa aatcattcca gaaatcttat aataagaatt
     6661 attttctaca ttgcttatat atgaaattat accatcatca aaataagtat ttccataacc
     6721 atactctaca ctatcacctt tacttattac tttaggtaca gcatatacta atgttatatt
     6781 ttttattgga gataacttat atgaaattga atttcctcct aaacttgtat ccattaaatc
     6841 ttctcttgct ttagataaca acatttgatt aataattttt tgatttactc cataagaatc
     6901 cacaatattt acttttccat ctttatcaaa attcattaca acgtagaatc tatagttttc
     6961 ttttatatct gtaatctgct tttgtctttc ctcatcattc atttctgtat tttttgactg
     7021 attttcatca gaattttctt ttgaagcatc attttttgtt gtatcaagta ctcctaattc
     7081 tttattatca ttaaaaaatt caaaattgct ttctttatca actaaatagt actttaaatt
     7141 ctttcctgac gctttaatga aatgctccca attttcaaag ttttcattaa aatctgcatt
     7201 aatactatca tctgtatagt aagtttctga accatcataa gtatcttttt ctttacttac
     7261 attttttata tagttcatag tttcttcatt gataaatagt tttgatggtt gtatcatctt
     7321 accttcttgt tcttccttca tattatgata taaaacataa cttcctttat atatactatc
     7381 tacaaagctt tcctgttcaa atggattctc tgctccacct ttagcacttg cttgtatagt
     7441 agaatattga ctaatcatac ctaatgatgc tgataacata agtataaaaa ctataatagt
     7501 aactatatat ttatctttaa attttttcaa ttttgtatcc aacaccccac accaccttta
     7561 aatatttagg atttttgggg tcaatttcta tcttttccct tatattcctc acatgtacca
     7621 taactgtatc tgtatttaca gcattctcat tccaaactct ttcatatatt tcatcagcag
     7681 aaaaaactct tcctgcattt ctcataagta aacttagtat tttaaattct ataggagttg
     7741 tttttatata ctttccatca atactaactt cttttgtatt ttcatttaat tctaatccgc
     7801 ctattgcaaa cacactatcg tcactttcta ccttttgctc tttatttcca accatgttta
     7861 gatacttagt gtaccttcta agttgtgaat taactctagc caatagttct aatggcttaa
     7921 aaggttttgt tacataatcg tctgctccta tattaagccc cattattttg tcaacttcct
     7981 ctgattttgc agatagcatt attactggaa aattatagtt ttctctaagc ttcattgtaa
     8041 gtgttatacc ctccatttta ggcatcatta tatcaactat agctaaatgt atttcttggt
     8101 tttctataac cttaagacct tctaatccat tgtatccctt aaaaacatta tatccttgat
     8161 ttaatagata tatttctata gcatctgcta tctccttttc atcttctacc accaaaatat
     8221 tatatgaatt cattctaatc tcctttatat taatttataa attttcttta catatatgat
     8281 tatacaatat gatagaagtt tttccattta tatatatatt tatatctttt gttctggtta
     8341 tttgtattta atgtccatct tagaaacctc cttctagttt aaatttaata ttttttcatt
     8401 ttgttttata tacttaat
//
LOCUS       DAELLV010000160         8376 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.160, whole genome shotgun sequence.
ACCESSION   DAELLV010000160 DAELLV010000000
VERSION     DAELLV010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8376
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.160"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(92..1768)
                     /gene="bclA2"
                     /locus_tag="KPD58_002704"
     CDS             complement(92..1768)
                     /gene="bclA2"
                     /locus_tag="KPD58_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA2"
                     /protein_id="HBG0266456.1"
                     /translation="MSDISGPSLYQDVGPTGPTGATGPTGPTGPRGATGATGANGITG
                     PTGNTGATGANGITGPTGNMGATGPNGTTGSTGPTGNTGATGANGITGPTGNTGATGA
                     NGITGPTGNKGATGANGITGSTGPTGNTGATGANGITGPTGNTGATGATGPTGLTGAT
                     GATGANGITGPTGNTGATGANGVTGATGPTGNTGATGPTGSIGATGATGTTGATGPIG
                     ATGPTGADGEVGPTGAVGATGPDGLVGPTGPTGPTGATGANGLVGPTGAVGATGANGL
                     VGPTGATGATGVAGAIGPTGAVGATGPTGADGAVGPTGATGATGANGATGPTGAVGAT
                     GANGVAGPIGPTGPTGANGVAGATGATGATGANGATGPTGAVGATGANGVAGAIGPTG
                     PTGANGATGATGATGATGANGATGPTGATGATGVLATNNAQFTVSSSSLVNNTLVTFN
                     SSFINGTNITFPTSSTINLAVGGIYNVSFGIRATLSLAGFMSITTNFNGVAQNNFIAK
                     AVNTLTSSDVSVSLSFLVDARAAAVTLSFTFGSGTTGTSAAGYVSVYRIQ"
     gene            complement(2146..2685)
                     /gene="hpt"
                     /locus_tag="KPD58_002705"
     CDS             complement(2146..2685)
                     /gene="hpt"
                     /locus_tag="KPD58_002705"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBG0266457.1"
                     /translation="MDIETKKWEVLYSEEKIKDKLRELGAIIEKDYKDKNLMVVSLLK
                     GSFIFCADLVRNINLPLRVNFMTTSSYGNNEESTGRVKVVSDVTTDIAGYDVLVVDDI
                     TDSALTMDFVLKHLKAKNPASLKCCVLLDKPSRRKVDLVPDYCGFEIEDKFVVGYGFD
                     FGDYYRNVPYIFNVTDEDR"
     gene            complement(3013..4320)
                     /locus_tag="KPD58_002706"
     CDS             complement(3013..4320)
                     /locus_tag="KPD58_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HBG0266458.1"
                     /translation="MRDIRKELVEMLKAEVKPAVGCTEPVALALACAKAKELLGEEIV
                     ENRMLVSPSIYKNGMCVGIPGTERLGLKIAAALGIVGGHSENGLSVLETLTKEEVKIA
                     EDYMDNTPLSITPADTREKVFIEVVLKGKNHIAKVRIRTKHDNFTFLEKDGEVLLDNE
                     PKVSASNDAAEKAESLMDTVTIQELIKNVEGIDFKDIEFLLDGVKMNEEMAEYGLKQK
                     TGIGVGYGIKKSIEEGLLGNDVINYAMMLTAGASDARMAGVKMPVMSSNGSGNHGLTA
                     ILPIVAYNKKFPQSDERLAKALAISHLVTGYIKNYTGRLSAVCGCGVAASTGATAGIS
                     WLMNGTEKQIEGAIENMIADLSGMICDGAKAGCALKLSSAASAAIQSAIIAKQDCFVP
                     PLNGIVGSSVEQSIQNLGRVSDKGMSITDEIILNVMNDMNKVD"
     gene            complement(4745..6520)
                     /locus_tag="KPD58_002707"
     CDS             complement(4745..6520)
                     /locus_tag="KPD58_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0266459.1"
                     /translation="MMIMIGLEYIKSDIQNIAEAIVSVLNIDVTIVNKELFRIAGTGV
                     YIDKIGEKVDKYTAFKKSLTEQITILIDDPKSSDICRECYKSGACVEFAEVCCPIICD
                     GDSHGVIGLIAFTKEQAQIIENNKSGLINFLGKMADLISNKLKAQIKTYELELEKKKL
                     ETLLNNMDKAVVSIDVDGKIDKYNSKFKELFNIKDSITGKNIFVELDFIKKPSINNFK
                     KDKSCSFFYKRYGYDSKGIYNINKIVLKNELKGYVIDFIDKRDAIKNYNKMNKDYRIK
                     LENIIGESVAIRNSKKEALIASKSTSTVLITGESGTGKELFARAIHNHSNRVDNPFIA
                     VNCAAIPDNLLESELFGYEEGAFTGAKKGGKLGMFEVAHKGSIFLDEIGDMSLHLQSK
                     LLRVLQEKELNKIGSKSNVSIDVRIIAATNKDLEKMVQNGTFREDLYYRLNVIPIKLP
                     SLRERKEDIPLIIKHMVKEYSKKLDKNVIDIDSRVVDAMMEYKWPGNIRELQNIIEYS
                     VNMSSSSIITMNVIPQKIKNTSVEELEVKSDRIIPLDELEKIEIIKALNKYKDYKKDK
                     ELTAKALGISRATLYRKIEKYNIIS"
     gene            complement(6517..6654)
                     /locus_tag="KPD58_002708"
     CDS             complement(6517..6654)
                     /locus_tag="KPD58_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266460.1"
                     /translation="MEINYELIIIVMLFVILMSIQFTLNKIYLILKEIRQILMLKKNK
                     E"
     gene            complement(6732..7301)
                     /locus_tag="KPD58_002709"
     CDS             complement(6732..7301)
                     /locus_tag="KPD58_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG0266461.1"
                     /translation="MKSILCQINKQPVKAKYTSDELECFLDTIEYNVGDKILLVGHIG
                     NLGKRLRGLGTSVTILENSNYEDVCYSLVYNENCNVVKGKLEFLPFDDNQFNKIIIVD
                     QFNVITDCEKASSEIQRVLKGNGEVILEDLNLKNLKVKLNYLKHKFCGSVAKIHYPHE
                     VFNIFSRLNFDGMIKESNKLKYIYVGKRR"
     gene            complement(7485..8120)
                     /locus_tag="KPD58_002710"
     CDS             complement(7485..8120)
                     /locus_tag="KPD58_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG0266462.1"
                     /translation="MIAVKDENNVVNLRGELDNKLEFSHEIFGEKFYNMKIKINRLSD
                     SFDILPMTVSERLLQDIDLDKQNLVNVVGQLRSYNKTLNNKNRLVLTVFVREIKSIDE
                     ENKDPNSIFLDGYVCKEPVYRKTPLGREITDLLVAINRPYNKSDYIPSIVWGRNAKFA
                     KNLKVGDRIQLWGRVQSREYEKKIDENNVVKKMAYEVSISKIKKLDENNNE"
BASE COUNT         2398 a         1665 c          954 g         3359 t
ORIGIN      
        1 ttttcatcat ataaacctat taaatattat tctatttatt ggtatatgaa atcatattgt
       61 ttattatttt ataatctttt catcatataa actattgtat tctataaact gatacatatc
      121 cagctgcaga agtacctgtc gtgcctgaac caaatgtaaa acttaaagta acagctgctg
      181 ctctagcatc aactaaaaag cttaaactta cacttacatc tgatgaagta agtgtattta
      241 ctgcttttgc aataaagtta ttttgagcta ctccattaaa gttagtagta attgacataa
      301 atcctgcaag tgaaagtgtg gcacgtatac cgaaagatac attgtatatc cctccaactg
      361 caagatttat agtactactt gttggaaaag ttatattagt tccatttata aatgatgaat
      421 taaatgtcac taatgtatta ttcactaaac ttgaagagga cactgtaaat tgtgcattgt
      481 ttgttgctaa cactcctgtt gctcctgtcg ctccggttgg acctgttgct ccatttgctc
      541 ctgttgctcc ggtcgcccct gttgctcctg tcgctccatt tgctccagtt gggcctgttg
      601 gtcctatcgc tcctgctact ccatttgctc ccgttgctcc tactgctcct gttgggcctg
      661 ttgctccatt tgcccctgtt gctcctgtcg ctcctgttgc tcctgctact ccatttgctc
      721 cggttggacc ggttggacct attggacctg ctactccatt cgctccggtt gctcctactg
      781 ctcccgttgg gcctgttgct ccatttgccc ctgttgctcc tgtcgctcct gttggaccta
      841 ctgctccatc tgctcccgtt gggcctgttg ctcctactgc tcctgttgga cctattgccc
      901 cagctactcc tgttgctcct gtcgctcctg ttggacctac caaaccattt gctccggttg
      961 ctcctactgc tcctgttgga cctaccaaac catttgctcc ggttgctcca gttgggcctg
     1021 ttgggcctgt tggacctacc aaaccatctg gacctgttgc tcctactgct cctgttggac
     1081 ctacctctcc atctgctccg gttggacctg ttgctcctat tgggcctgtt gccccagttg
     1141 ttccggttgc tccagtcgct cctatacttc cggttggacc tgttgctcct gtatttcctg
     1201 ttggtcctgt cgctcctgtt actccatttg ctccggttgc tcctgtattt cctgttggac
     1261 ctgttattcc atttgctccg gttgctccgg ttgctccagt tagtccggtt ggacctgttg
     1321 ctccagttgc tcctgtattc cctgttggtc ctgttattcc atttgctcca gtcgctcctg
     1381 tatttcctgt tggtcctgta gagcctgtta ttccatttgc tccagttgct cctttgtttc
     1441 ctgttggtcc tgttattcca tttgctccgg ttgctcctgt attccctgtc ggacctgtta
     1501 ttccattcgc tccagtcgct cctgtatttc ctgttggtcc tgtagaacct gttgttccat
     1561 ttggtccagt cgctcccata tttcctgttg gacccgttat tccattcgct ccggttgctc
     1621 ccgtatttcc tgttggacct gttattccat ttgctccggt cgctccagtt gcacctctag
     1681 gccccgtcgg tcctgttgga ccagtagcac ctgttggccc tgttggacct acatcttgat
     1741 ataaacttgg acctgaaata tcactcatat aattaatcct ccttttttaa agttagagta
     1801 ttactctata aatattgagt attactctat ataattatat gaatcgacaa tttcttatgt
     1861 gataatgtat ctatttgctt atatcaacat atatgctttg attcttgaat ttcattactt
     1921 ttttgtaaaa tcttttacaa ttattagtac tcttttacaa ttattaatac aattaaaaaa
     1981 ctcatttccc atcaattatt taaaattttt ttattatgtt ataaaaaata gataattaaa
     2041 ttgcataaaa ttaatttaat actactttaa ttacttttta aataaaaaaa cctcaacacg
     2101 attaataaaa atcatattaa ggcatccaca aaattatata attgcttatc tatcttcatc
     2161 agtaacattg aatatataag gaacatttct ataataatca ccaaaatcaa acccatatcc
     2221 aaccacaaac ttatcttcta tttcaaatcc acaataatct ggcactaaat cgacttttct
     2281 tctgcttggt ttatccaaaa gaacacagca ttttaaacta gcaggatttt tagcttttag
     2341 atgttttaac acaaaatcca tagtaagtgc agaatcagta atatcgtcta caaccaaaac
     2401 atcatatcct gctatatcag tagtcacatc tgaaactact ttaacccttc cagtactttc
     2461 ttcattgttt ccataactag atgtagtcat aaaattaact ctaagtggta aatttatatt
     2521 tctaactaag tctgcacaaa atataaagct accttttaat agagaaacta ccataagatt
     2581 tttatcttta taatcttttt ctataattgc tcctaattcc cttaatttat ctttaatttt
     2641 ttcttctgaa tacaatactt cccatttttt tgtttctata tccataaaaa gacctccaat
     2701 tgtaataatc cttcttaatt ttataagaga ttgctcactt tttcaatact cttattcaat
     2761 attagtcaat attattgagt ttttatgttc attcaacaat tattaacaat gtatcttata
     2821 ttttaagtag ttcatatatc cacactaaat tacatactat atattttaga caaaaaaaga
     2881 aaaaacttgc actaacaagt tttttctatt atacataaat ttatacataa tcatatttta
     2941 ttagagggta ctaattaaat ttatttatgc tatttttgtt atacctcata atacaaaaca
     3001 tattataaag tactagtcaa ctttattcat atcattcatt acatttaaga ttatctcatc
     3061 tgttatagac atacctttgt cacttactct acctaagttt tgtatacttt gctcaacact
     3121 tgaaccaact ataccattta gtggtggtac aaaacaatct tgcttagcta ttatagcact
     3181 ttgtatagct gcagatgctg cagaagataa ttttaatgca caaccagctt tcgcaccgtc
     3241 acatatcatt ccacttaagt cagctatcat attttctata gctccttcta tttgcttttc
     3301 agtaccattc ataagccaag agataccagc agttgctcca gtagaagcag caacaccaca
     3361 tccacataca gcagataatc ttcctgtata gttcttaata taaccagtta ctagatgaga
     3421 tatagctaaa gcttttgcta gtctttcatc agattgtggg aattttttat tataagcaac
     3481 gattggaaga attgctgtta atccatgatt tccacttcca tttgaactca taacaggcat
     3541 ttttacccct gccattcttg catctgaagc tccagcagtt aacatcattg cataatttat
     3601 aacatcatta cctaaaagac cttcttctat agacttttta attccatatc ctacaccaat
     3661 accagttttt tgttttaatc catattcagc catctcttca ttcattttta ctccatctag
     3721 taaaaattct atatctttaa aatcaattcc ttctacattt tttattaatt cttgtatagt
     3781 tactgtatcc attaaacttt cagctttttc tgccgcatca tttgaagctg atacttttgg
     3841 ttcattgtct aataatactt ctccatcttt ttctaagaac gtaaaattat catgtttagt
     3901 tcttattctc actttagcaa tatgattttt tccttttaac acaacttcta taaatacttt
     3961 ctctcttgta tcagcaggtg ttattgatag tggagtatta tccatataat cctcagctat
     4021 tttaacttcc tcttttgtta gagtttctaa cacacttaat ccattttcag aatgtccacc
     4081 tactatacct agagctgctg caatttttaa tcctaatctt tcagtacctg gtatacctac
     4141 gcacatacca tttttataaa tactaggact aactaacata cgattttcaa ctatttcttc
     4201 tcctaataat tcctttgcct ttgcacatgc tagagctaag gctactggtt cagtacaccc
     4261 tactgctggt ttaacttctg cctttaacat ttccactaat tcttttctta tatctctcat
     4321 tatatataaa cctcccaatt aagtttagat ttagtactca aataattaat taaataattt
     4381 cttatctata tttatagcaa aatacatgcc atatttttta tatcttttta tatttttagt
     4441 cttatattct ataaccattg gtatgactgc ttctatttat gtattttttt tatttacata
     4501 gagtatttat attctttaaa atggagaaac atatgacaat ttcgcaaaaa gacaaaaaaa
     4561 agtctcaaaa tgagattcta tatcattttg agacggctca aatgtattat tttttaactt
     4621 ataaaaacca ttttttaatt cataaaaaaa gattctgcaa aacacttata gtataagttt
     4681 aaagctttct ttaaagttat gaatttagaa aaatttaaat tttatatagc tttttattat
     4741 attactatga aattatattg tatttttcaa tttttctata taatgtagct cttgatattc
     4801 caagtgcttt agctgttaat tctttatctt ttttataatc tttgtactta tttaaagctt
     4861 ttattatctc aatcttctct agttcatcaa gtggaattat cctatcgctt ttaacttcta
     4921 actcttctac agaggtattc ttaatttttt gaggaattac attcattgtg attattgaag
     4981 aagaagacat attcacacta tattcaataa tattctgcaa ctctcttata ttccctggcc
     5041 acttatattc catcatagca tcaacaaccc tgctatctat gtcaataaca tttttatcta
     5101 atttttttga atactcttta accatatgct tgattataag tgggatatct tcttttctct
     5161 ctcttaaact tggtaatttt ataggtataa catttaatct atagtataaa tcttctctaa
     5221 aagtgccatt ttgaaccatt ttttctaaat ctttatttgt agcagcaata attcttacat
     5281 ctattgaaac atttgattta cttccaattt tattaagttc cttttcttgt aatactctca
     5341 ataactttga ctgtaaatgc aaactcatat ctcctatttc atctaaaaat atacttccct
     5401 tatgagctac ttcaaacatt ccaagctttc cacctttttt agctccagta aaagctccct
     5461 cttcatatcc aaataattca ctctctaata aattgtctgg tatagcagca cagtttacag
     5521 ctataaaagg attatctact ctattgctat ggttgtgtat ggctcttgca aatagctcct
     5581 tacccgttcc actttcccca gtaataagta ctgttgatgt tgatttagaa gctattaggg
     5641 cttccttttt agaatttctt atagctacac tttctccaat tatgttttcc aattttattc
     5701 tataatcttt attcatctta ttgtagtttt ttatagcatc tctcttatct ataaaatcaa
     5761 taacatatcc ctttaactca ttttttaata ctattttatt gatgttgtat attcccttcg
     5821 aatcatatcc gtatctttta taaaaaaaac tacaggattt gtccttctta aagttattta
     5881 tagaaggttt ctttataaaa tctaattcga caaatatgtt tttgccagtt atactatctt
     5941 ttatattgaa aagctcttta aattttgaat tatatttatc aatttttccg tccacatcaa
     6001 tagacacaac agctttgtcc atattgttta atagtgtctc taattttttc ttttctaatt
     6061 caagctcata agttttaatt tgtgctttta atttattaga tattaggtct gccattttgc
     6121 ctaaaaagtt tataagacca cttttattat tttctataat ttgtgcttgt tcttttgtaa
     6181 aggctataag gcctataaca ccatgagaat cgccatcaca tataatagga caacacacct
     6241 ctgcaaactc tacacaagcc ccacttttat agcattccct acatatatca ctactctttg
     6301 ggtcatcaat tagtattgta atctgttctg taagtgattt tttaaaagcc gtatatttat
     6361 ctactttctc acctattttg tctatatata ctcctgtacc tgcaatccta aataactcct
     6421 tatttactat agttacatct atattgagta ctgatactat agcttcagct atattttgaa
     6481 tatcagattt tatatattct aaacctatca taatcatcat tccttatttt tctttagcat
     6541 aagtatttgt cttatttctt taagaatcaa gtatatctta tttaaagtaa attggattga
     6601 catcaggatt acaaagagca taacaattat tattaactca taatttattt ccatctaaac
     6661 atctccttat aacaaaagac taggcttaac ctagtctttt atattatatc catatttata
     6721 ttttaactca tttatcttct ttttcccaca taaatatatt ttaatttatt tgactcttta
     6781 atcataccat caaagtttaa tcttgaaaat atattaaata cttcatgtgg atagtgaatc
     6841 tttgcaacag aaccacaaaa tttatgttta agatagttta acttaacttt taaattcttt
     6901 aaatttaaat cctctaatat aacctctcca ttgcccttta acactctttg tatttcagat
     6961 gaagcttttt cacaatctgt aataacatta aattggtcaa ctattattat cttgttgaac
     7021 tgattatcat caaatggtag aaactctaac ttacctttaa ctacattgca attttcatta
     7081 tacactagag aataacagac atcttcataa ttactattct ccagtatagt tacagaagtt
     7141 ccaagacctc tcaatctctt gcctaggttt cctatatgac caacaagcaa tattttatca
     7201 cctacattat actcaattgt atccaaaaaa cattctaatt catcagatgt atatttagct
     7261 ttaactggct gtttatttat ctgacataat atgcttttca caatgcttca cctcttcatt
     7321 tcctcaatta taaattatgt acagattaca ttataataaa tggcgagcaa ctattcaaga
     7381 gatatttcat tagtattttt ttaaacaatc tttatacatt ttaatatgat attataccaa
     7441 atttattagg attttattca taaaaatcag tctatttttt atgtttattc attgttattt
     7501 tcatccaatt tttttatttt cgatatggat acttcatatg ccattttttt gaccacatta
     7561 ttttcatcta tttttttctc atattctcta ctttgaaccc taccccaaag ctgtattcta
     7621 tcaccaacct ttagattctt tgcaaattta gcatttctac cccaaactat tgatggtata
     7681 taatcagatt tattataagg tctgtttata gcaactaata aatcggttat ttcccttcct
     7741 aaaggtgtct tcctatacac aggttcctta catacatatc catctaaaaa tatactatta
     7801 gggtctttat tttcctcgtc tatacttttt atttctctga caaacactgt aagcactaat
     7861 ctatttttat tatttaatgt tttgttatat gatcttaatt ggcctacgac attgactaaa
     7921 ttttgtttat caaggtcaat atcttggaga agtctttctg atacagtcat tggaagtata
     7981 tcaaaagaat cgcttagtct gtttattttt attttcatat tataaaattt ttctccaaat
     8041 atctcatggc taaattctag cttattgtct aactcccctc ttaagtttac tacgttattc
     8101 tcatctttaa cagctatcat attttatccc tccacaattg ttaattctta tatttctggt
     8161 atcagcttat ataataaata tatttataag ggatgtattt tattacaaat ctttgattgc
     8221 tgtttaaaag tttttattta gttataaatt tcatttaata tttcactttt ataattataa
     8281 aattattatc tttttgtacc acatatacta attaaactac ctatttcagt tttgtattaa
     8341 ttataaaaat cctattatat aattaataag taatat
//
LOCUS       DAELLV010000161         8346 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.161, whole genome shotgun sequence.
ACCESSION   DAELLV010000161 DAELLV010000000
VERSION     DAELLV010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8346
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.161"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..177
                     /locus_tag="KPD58_002711"
     CDS             <1..177
                     /locus_tag="KPD58_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG0266463.1"
                     /translation="TRVLLEKCYEKNVIFMPGDLFFTDNSGFDTLRLGFSRLSFEDIE
                     IGIKIIGETINEMF"
     gene            236..1042
                     /locus_tag="KPD58_002712"
     CDS             236..1042
                     /locus_tag="KPD58_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG0266464.1"
                     /translation="MNLILGDNMSKKSKRRKINKLKLTIVITVLFLALLSIYEILFSN
                     PNKKGNIKSQETMQNKNNTTDDSKNNQSSTKKQEDNNIKPTFSYSSIPDNIKNKMIGK
                     SMPTDEPISFDSLSYLKLTYYGFDEKTHQGEMIVNSELAPEVVDIFKELYEKKYPIEK
                     IKLIDDYDAVDEKSMSDNNTSSFCYRTIAGTNVVSNHGKGRAIDINPLQNPQVSGNDV
                     TPKVSTVYADRSSTKFGMIKKGDDCYNAFVSRGWSWGGYWKNPDYQHFEK"
     gene            1068..2987
                     /locus_tag="KPD58_002713"
     CDS             1068..2987
                     /locus_tag="KPD58_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071223118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG0266465.1"
                     /translation="MNLMTLENISKSYSEKKLLENISLGINEGEKIGLIGVNGTGKST
                     LLKIIAGVEESETGNITKTNGIRIEYLPQNPTYNENSTVLEQVFNGTSEELKLLGEYQ
                     EILEKLNSNFTEELNKKLLSVHEKIDALNLWDLESEAKSVLTKLGINDFNQKIAELSG
                     GQRKRVSLASALITPCEFLILDEPTNHLDNDTIDWLEEYLNSRKGSLLMITHDRYFLD
                     RVTNRIIELDKGRLFSYDGNYSIFLEKKMERLSLEASIEDKRQNLIRTELAWVRRGAQ
                     ARSTKQKARLQRFDELINRDSFKPDENLDISVASSRLGNKIIEIHNISKSFEENTVID
                     NLEYTLTRTDRIGIIGKNGMGKSTLINILNGEIEPDSGYISIGETVKIGCFSQDDSHM
                     NIDMRAIDYVKEASDYIETSDGTRITASQMCERFLFNGTMQYTLIGKLSGGERRRLHL
                     LKILMTAPNVLLLDEPTNDLDIETLKILEEYLDEFKGVVVTVSHDRYFLDRICNKIFA
                     YEGNGKIHIYTGNYSDYLIYREIQGIEFEESKRESVKKDDSTTPKKEKPKNDKKLKFS
                     YNEQREFENIDSDIEKLESKIATLDESTAKFSTDFTKLQEILDEKSKLEKELEYKYER
                     WEYLNNLADEIANNK"
     gene            complement(3229..3576)
                     /locus_tag="KPD58_002714"
     CDS             complement(3229..3576)
                     /locus_tag="KPD58_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HBG0266466.1"
                     /translation="MSKSNDVLDIVFKITDGLEYEVAENRVVTILEKQDHKIQKFFRK
                     LKARIPKYKKIELDEYSSFVFLQIDGQRTVREIGKNLEAKYGEESHPLYERLLVFLNH
                     IEVNCHYIERLDI"
     gene            complement(3563..5491)
                     /locus_tag="KPD58_002715"
     CDS             complement(3563..5491)
                     /locus_tag="KPD58_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HBG0266467.1"
                     /translation="MNKKLPKGAYGEVSGKDYVPYITDKSRTGGNVAVLIIGIILAAI
                     FAASTTYSGMKAGLTVAAGIPGAIIGSAFVGAFARSKGILGKNLIQGMSSGGESVASG
                     FIFVLPAVILIGSQITFLEGLAVGVGGVLFGIGIAAIVHNYLIVEEHGKLMYPESMAI
                     SETLVASEAGGDSIKYMGIGFVISGFITVLTGSFLNVANNVMSLVGSKFYKWKFDIEV
                     NPLLLGIGFIVGLEVSLTMFAGSILSNFGIAPLIGYFTDMAKDGAMVWNNPAMPLNQM
                     DVGAISSSYVKYIGAGMMLCGGIIGAIKLIPTIIASIKETLKAKSSAGEGEEGSSIQM
                     ILLLGGVVIGFLAAFLISGNIVMAIIGAIISLLLSLLFVIVAGRLTGTIGTSNLPVSG
                     MTIASLVIVTLVFVIMGWTDLEANKSLLLFGSFIVVAIAIAGGYTQSQKVTYIIGGSK
                     NEMQRYFTIASIVGVIVVVGVILLLSDQLRATGDNVQFALPQANLMSTLTSGIMSGSL
                     PWVMIIVGVFMAIVLYALNLPIMTIAIGFYLPIATTSIILVGALIRLFVELVSKTEKE
                     KEVKVSNGISLSSGLVAGGSIIGLIGIILQVTGVVTPKVPSGFAATNSMAIALLVVLV
                     VLTALPIVLSKVKNNEQE"
     gene            complement(5903..8092)
                     /locus_tag="KPD58_002716"
     CDS             complement(5903..8092)
                     /locus_tag="KPD58_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HBG0266468.1"
                     /translation="MYEVNLERAMNYWMDLLDDYTNIAKIPNDFFYKDCSDEVVDEVF
                     KMNIEKHERLIQYSKVNNISIDSIIETIYGLILQKYIYEDDVVFGKTMNIIPIRVRVR
                     QSEKFIDVVRKLKRQWSRSLEYGYYLLSDIENKNHLNTKLINTIFIAKSFDLSYDYDI
                     EKKGYDLSVIVYTKRALKIKIKYKPSIYKRETIRRILNQFDYIIEQIIEDNDILIEDL
                     EFVCKEEEKVLLEDFNNNQYEVPYDKSYVDLFREQVKVTPSNIAARDENRKLTYKELD
                     EVTDKLAGYLNHIGVESEDIVAVMLPRDINIIITAIGIMKSGGAFFPIDTSNPEERLN
                     YLLEDSNAKVVITTDELKSKVVNENTIVLDINDEEMFKFGYELTEKITSSNCAYTIST
                     SGSTGRPKTIAIEHKSLVNMCYYSVKSISATENDICGIYLSFSFDAVMKQLFPYLLVG
                     ASIDIMPEEAKFDEYTVNEYCEENDITILALPTAFARLFIQNCNNNSLRVVQTGGERL
                     KGYRKRNYELYNEYGPTEFTVVSTSFHVDREYGKIPIGKPIFNTYAYVLDKKNKLCPI
                     GAPGELCLSGIQISRGYLNKKGLTEQVFVENPYKTCEHNKLMYRTGDLVRWLDDGNLD
                     YIGRMDNQVKIDEFRIELYEIENIINNITEIKSVVCISRTNDDGDIYICAYYVIDEED
                     SGKINERTIREYLNEHLPPYMIPTIIMRIDKIPVTPIGKVNKRALPE"
BASE COUNT         3055 a         1306 c         1057 g         2928 t
ORIGIN      
        1 actagagttt tattagaaaa atgttatgaa aaaaatgtta tatttatgcc tggagattta
       61 ttttttacag ataactctgg ttttgatact ttaagactcg gattttctag gttgtctttt
      121 gaagacattg aaattggaat aaaaataatt ggagaaacta taaatgaaat gttctaggtt
      181 aactaaatat tgttagccta gtttattttt atgaggtata atatatatag tttatatgaa
      241 tctaatactg ggggataata tgagtaaaaa gtcaaaacga agaaaaatta ataaattaaa
      301 attaacgatt gtaataactg tattatttct tgctttatta tctatatatg agatactgtt
      361 ctctaatccc aacaaaaaag gtaatataaa gagtcaagaa actatgcaaa ataaaaataa
      421 tacaacagat gattctaaaa ataatcaaag ctctaccaaa aagcaggaag ataataatat
      481 caagcctact ttttcatatt cttcaatacc agataatata aaaaataaaa tgattggtaa
      541 atctatgcct actgatgaac ctataagctt tgatagtcta tcttatttaa aacttacata
      601 ttatggattt gatgaaaaaa ctcatcaagg agaaatgata gtaaacagtg aactagctcc
      661 agaagtagtg gatattttta aggaattata cgaaaaaaaa tatccaattg aaaaaattaa
      721 gttaatagat gactatgatg ctgtagatga aaaatctatg tctgataata atacctcatc
      781 attttgttat agaactatag ctggtacaaa tgtagtttca aatcatggta aaggacgtgc
      841 tatagatata aacccattac agaatcctca agtttctgga aatgatgtta cacctaaagt
      901 aagtacagtt tatgctgata ggtctagcac aaaatttgga atgataaaaa aaggtgatga
      961 ttgttataat gcttttgtaa gcagaggttg gagttggggt ggctattgga aaaacccaga
     1021 ttatcaacat tttgaaaaat aatagattag aaataaaggt gaattatatg aatttaatga
     1081 cattggaaaa tataagtaaa agctattctg aaaaaaaatt actagaaaat atatctcttg
     1141 gaataaatga aggggaaaaa ataggactta ttggtgtaaa tggtactggt aaatctacct
     1201 tactcaaaat tattgcagga gttgaagaaa gtgagactgg taatataaca aaaactaatg
     1261 gtattaggat agaatatcta cctcaaaatc caacttacaa tgaaaattca actgtacttg
     1321 aacaagtatt taatggaact tctgaagaat taaagctact tggtgaatat caagaaatcc
     1381 tagaaaaact taattcaaac tttacagagg aattaaataa aaaattatta tcagtacacg
     1441 aaaaaataga tgccttaaat ctttgggatt tagaaagtga agctaagtca gtacttacaa
     1501 agcttggtat aaatgacttt aatcaaaaaa tagcagaact ttctggtggg caaagaaaga
     1561 gagtctctct ggcatcagcc cttattacac cttgtgagtt tttgatacta gatgagccta
     1621 caaaccatct agacaatgat actatagatt ggcttgagga atatcttaat tccagaaaag
     1681 gctcattgct tatgataact catgatagat actttttaga ccgtgttaca aatagaatta
     1741 ttgaactgga caaaggtaga ttattcagtt atgatgggaa ttattcaata tttttggaaa
     1801 agaaaatgga aagactttcg ctagaagcaa gtattgaaga taaaagacaa aatttaataa
     1861 gaacagagct tgcgtgggta agacgaggag ctcaggctcg tagcactaaa caaaaagctc
     1921 gtttacaaag atttgatgaa cttataaata gagattcttt taaacctgat gaaaatcttg
     1981 atatatctgt tgcatcaagt agacttggaa ataaaataat agaaatacac aatatatcta
     2041 aatcatttga agaaaatact gttatagata atttagaata cactttaact cgtacagata
     2101 gaattggtat aataggcaaa aatggtatgg gtaaatctac ccttataaat atacttaatg
     2161 gagaaataga gcctgatagt ggatatattt caattggaga aacggttaaa ataggttgtt
     2221 tttctcaaga cgactctcat atgaatattg atatgagggc tatagactat gtaaaagaag
     2281 ctagtgacta tatagaaact tcagatggta ctaggattac agcttctcaa atgtgtgaaa
     2341 gattcctttt taatggtact atgcaatata ctttgatagg taaattatct ggtggtgaaa
     2401 gaagaagact acatcttctt aaaatcctta tgacagctcc taatgtgctt cttttagatg
     2461 aacctacaaa tgatttagat attgaaacgc ttaaaatatt agaagaatac ctagacgaat
     2521 ttaaaggtgt agtagttaca gtatcacatg atagatattt cttagataga atttgtaata
     2581 aaatatttgc atatgaaggt aatggaaaaa ttcatatcta tacaggtaat tacagtgatt
     2641 atcttattta tagagagatt caaggaattg aatttgaaga atctaaaagg gaatcagtta
     2701 aaaaagatga ctctactacc ccaaaaaaag aaaaacctaa aaatgataaa aagcttaagt
     2761 tctcatataa tgaacaaagg gagtttgaaa atattgactc tgatatagaa aaactcgaat
     2821 ctaaaattgc aacacttgat gaaagtactg caaaattttc aactgatttt actaaactcc
     2881 aagaaatttt agatgagaaa tctaaactag aaaaagaact tgaatacaaa tatgaaagat
     2941 gggaatattt aaataatcta gctgatgaaa tagctaataa taagtaatat taaaataatc
     3001 ttaagtttaa taagttttgc tttaaatctg taatttataa atattttaaa ggaagtgtct
     3061 caaaatgaac tttttagttt tatgagacac tcttttttat gagaaatcaa atatattttc
     3121 atcatttcaa caatacaaaa gaggcttatc tctattttga gacagcctct ttcataatta
     3181 ccttctaatt tatatgctct aatatatcta taataattct tattatatct atatatccag
     3241 tctttcaata tagtgacaat taacttcaat atgatttaaa aatactaaca acctttcata
     3301 aagtggatga gattcttctc catatttagc ttctaggttc ttgcctattt ctctaacagt
     3361 cctttgacca tcaatttgta aaaaaacaaa actggaatat tcatctagtt ctattttttt
     3421 gtatttaggt attctagctt ttaactttct aaaaaacttt tgaattttat ggtcctgttt
     3481 ttcaagtata gtaacaacac gattttcagc tacttcatat tcaagtccat cagtaatttt
     3541 aaatacaata tctaaaacat cattactctt gctcattatt ttttacttta gataaaacaa
     3601 taggtaatgc tgtaagcaca actaaaacta ctaataaagc aattgccatt gaatttgttg
     3661 cagcaaatcc acttggtacc tttggagtta caactccagt tacttgtaat attataccta
     3721 ttaaaccaat gatagaacca ccagcaacaa gaccagaaga taaacttata ccattagaaa
     3781 ctttaacttc tttttctttt tcagttttag atactaattc aacaaataaa cgaattaatg
     3841 ctccaactaa tattatagaa gtagttgcaa ttggtaaata gaatcctata gctatagtca
     3901 ttattggtaa atttaatgca tatagaacta ttgccataaa tactccaaca ataatcataa
     3961 cccaaggtaa actacctgac ataataccag aagttaatgt tgacattaag tttgcttgtg
     4021 gtaatgcaaa ttgtacatta tcaccagttg ctctaagttg gtcagaaagt aataaaatta
     4081 ctcctacaac aactataaca ccaactatac ttgcaattgt aaagtaacgt tgcatttcgt
     4141 ttttgctacc accaataata taagttactt tttgagattg agtataacct ccagctatag
     4201 caatagcaac aactataaat gaaccaaata aaagtaatga tttattagct tctaaatcag
     4261 tccatcccat tataacaaac actaatgtta caataactag agaagctata gtcataccag
     4321 aaactggaag atttgatgtt ccgattgttc ctgttaaacg accagctact atgacaaata
     4381 ataaagataa taaaagagat atgatagctc caataattgc cataacaatg ttaccagaaa
     4441 ttaagaatgc tgctaaaaat ccaataacta ctccacctaa taaaagtatc atttgtatag
     4501 atgaaccttc ttctccttct ccagcacttg atttagcttt aagtgtttct tttatagaag
     4561 cgattatagt tggtataagt tttattgcac caataatacc tccacaaagc atcataccag
     4621 cacctatata ttttacataa ctactagata ttgctccaac atccatttgg ttaagaggca
     4681 ttgcaggatt attccatacc atagctccat ctttagccat atctgtaaag tatcctatta
     4741 aaggtgctat accaaagttt gataaaatac ttcctgcaaa cattgttaaa gaaacttcaa
     4801 gccctactat aaatccaatt cctaaaagta gaggatttac ttcaatatca aatttccatt
     4861 tgtagaattt gcttcctaca agactcatta cattgtttgc tacatttaag aatgaaccag
     4921 ttaatacagt aataaatcca cttattacaa atccaattcc catgtactta attgaatctc
     4981 caccagcctc tgaagcaaca agtgtctcag atatagccat tgattctggg tacataagtt
     5041 taccatgttc ttcaactatt agatagttgt gaactatagc agcaatccct ataccaaata
     5101 aaactccacc aactccaaca gctaagcctt ctaagaatgt aatttggctt ccaattaata
     5161 taactgctgg taatacgaaa ataaatccac ttgcaacaga ttctccacca cttgacatac
     5221 cttggattaa gtttttacca agaataccct ttgaacgagc aaatgctcca acaaaagcag
     5281 aacctattat agctcctgga atacctgcgg caactgtaag accggctttc attccagagt
     5341 aagttgtaga tgcggcaaaa atggcagcta aaataatacc gataatcagt acagctacat
     5401 ttccaccggt tctggactta tctgtgatat atggaacgta atcttttcca ctaacctcac
     5461 cataagcacc cttaggtaat tttttgttca taaaattttc accccttttc aatttgtata
     5521 tattataaca tatatacata taaaacttaa ctttttatta ttttacaaat agcgagtcaa
     5581 aactactaaa atttaaccct atattattaa tttctacttt tagatatttt tatgctaatt
     5641 ttctttttat ttttcaattc tactaaaaac tattaagata taaaataaaa aaatttttta
     5701 atgtaaaatt aattttgtaa aaaaaatgct ttaaaacacg gaaaagtata aaaaatacaa
     5761 taatcattct tataaataaa ttttcaccat agtaaatata tacagaaaat tcttgttttt
     5821 atgcaaattg attattttat aaaaataagt gtcataaaga aatgtctgaa atcactttat
     5881 gacacctatc ataataaaaa aattattctg gtaatgctct tttatttact tttccgattg
     5941 gtgtaactgg tatcttatct atcctcatta ttattgttgg aatcatatat ggaggtagat
     6001 gctcatttaa atattctcta atagttcttt catttatctt accactatct tcctcatcaa
     6061 ttacataata tgcacatata taaatatctc catcatcatt tgttctcgat atacaaacaa
     6121 cacttttaat ttctgtaata ttatttatta tattttcaat ctcatatagc tctatcctaa
     6181 actcatcaat ttttacttga ttgtccatcc ttccaatata atctaaattt ccatcatcta
     6241 accatcttac taaatcacca gttctataca ttaacttatt atgttcacaa gttttgtaag
     6301 gattctctac aaatacttgc tctgttagcc cttttttatt taaatatcct cttgatattt
     6361 gaatgcctga tagacatagc tctcctggtg ctccaattgg acatagtttg ttctttttat
     6421 ctaatacata tgcataagta ttaaatatag gttttcctat tggtattttt ccatattctc
     6481 tgtccacatg aaaagaagtc gatacaacag taaattcggt tggtccatac tcattatata
     6541 gttcataatt acgctttcta tatcctttta atctttctcc tccagtctgt acaactctaa
     6601 gtgaattatt attgcaattt tgtataaata accttgcaaa tgctgttggc aatgctagta
     6661 tagttatgtc attttcctca cagtattcat ttactgtata ttcatcaaat ttagcttctt
     6721 caggcataat atctattgat gcacctacaa gcaaataagg gaatagttgt ttcattacag
     6781 catcaaaact gaaacttaag tatataccac atatatcatt ttctgtagcg cttatagatt
     6841 ttacgctata ataacacata tttactaaac tcttatgttc tatagctatg gtctttggcc
     6901 taccagttga ccctgaagta gatatcgtat atgcacaatt tgatgatgtt attttttcag
     6961 ttaattcata tccaaactta aacatttctt catcatttat atcgagtact atggtattct
     7021 catttacaac cttacttttg agttcatctg tagttatcac tactttcgca ttactatctt
     7081 caagaaggta attcaatctt tcttctggat tacttgtgtc tattggaaaa aatgctcccc
     7141 ctgatttcat aatccctatt gctgtaataa ttatgttaat atctcttggt aacattacag
     7201 ctactatgtc ttcactttcc actcctatat gatttaaata acctgcaagt ttatcagtta
     7261 cttcatccaa ttctttatat gttaactttc tattttcatc cctagcagct atattactag
     7321 gtgttacctt aacttgctcc ctaaataaat caacataact tttatcatag ggtacttcat
     7381 attgattatt attaaagtct tccaataata ctttttcttc ttccttacat acaaattcta
     7441 aatcttctat taaaatatca ttatcttcta ttatttgctc tattatataa tcaaattgat
     7501 ttaaaattct tctaatagtt tctcttttat atatagatgg tttatacttt atttttattt
     7561 taagtgccct cttagtatat actattacag ataaatcata tccttttttt tctatgtcat
     7621 aatcatatga taaatcaaaa cttttagcta taaatattgt gtttataagc ttagtattta
     7681 gatgattttt attctctata tctgataaca aatagtatcc atactctaaa gacctgctcc
     7741 actgcctttt tagcttcctt acaacatcta taaatttttc actttgtcta actcttactc
     7801 ttattggaat tatattcatt gttttaccaa aaactacatc atcttcatat atatactttt
     7861 gaagtataag gccatatata gtttctatga tagagtctat gctaatattg ttcactttag
     7921 aatattgaat taacctttca tgtttctcaa tattcatctt aaaaacctca tctactactt
     7981 catcagaaca atctttataa aaaaaatcat ttggaatttt tgctatattc gtataatcat
     8041 ctaacaaatc catccaataa ttcatagctc gttctaaatt aacttcatac atatcaaaat
     8101 tcccccttac tactaagtct atagaaaaaa ttcttttgga attttatttt cacaatatat
     8161 aaatatatta ataatacata tgcaagctct aaaaacatga gcttgcataa aattacactt
     8221 attatgacta ctaaattcgt aatatttaca atgtttaaaa tatattataa cacatatatg
     8281 attttatttt tctacattta tataatatat aaattacaaa tagttattta actaatatat
     8341 aacaat
//
LOCUS       DAELLV010000162         8325 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.162, whole genome shotgun sequence.
ACCESSION   DAELLV010000162 DAELLV010000000
VERSION     DAELLV010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8325
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.162"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            114..269
                     /locus_tag="KPD58_002717"
     CDS             114..269
                     /locus_tag="KPD58_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266469.1"
                     /translation="MKGILKELTAKLYSFFYFMFWGFYFSAGYFFYKNICTSFLVRPY
                     FSDFEHK"
     gene            399..1412
                     /gene="aroF"
                     /locus_tag="KPD58_002718"
     CDS             399..1412
                     /gene="aroF"
                     /locus_tag="KPD58_002718"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBG0266470.1"
                     /translation="MIVVLKMGADKNEVKKLIEAIGREGVEVNPIDGTELTVLGLVGD
                     TSKIDAKRIEANKVVEKVMHVVEPFKKANRKFHPEPSIINVNGMEIGSKKIAMIAGPC
                     SVETEDQIVSIAKDVKKSGAGFLRGGAFKPRTSPYAFQGLKYDGLDLLKKAKEKTGLP
                     IVTEIMSTQDIDIFEENVDVIQVGARNMQNFDLLKELGKTNKTILLKRGLSATIEEWL
                     MSAEYIMAGGNENVVLCERGIRTFETYTRNTLDLSAILAVKKLSHLPVIVDPSHAAGK
                     SWMVDSLSKAAIAVGADGLIIEVHNDPAHALCDGKQSIKPNEYDELISELKTIASAVG
                     REI"
     gene            1434..2498
                     /locus_tag="KPD58_002719"
     CDS             1434..2498
                     /locus_tag="KPD58_002719"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBG0266471.1"
                     /translation="MEEKLLVNLKENSYNIFIKKNILKNIGEEIKKIYFGNKIFIVTD
                     ENIAKLYLDKVKNNLSESGFEIKSLILKSGEETKSFNTLPKIYDSLLDFKLTRKDLII
                     TLGGGVIGDLGGFAAATFLRGISFIQIPTSLLAQVDSSVGGKVGVDLERGKNLVGSFY
                     QPKAVFIDPNVLLSLPEKFYRDGMAEVIKYGCIKDKTFFDKLLSLKNREEVMQHIEYI
                     IHKCCFIKKTVVEADEKDFGDRMLLNFGHTLGHAIEKYYNFTGYTHGEAVAIGMYKIT
                     LESEKIGITKKGTSELIKSILMNYNLPYEIELRDNKSIVDAISLDKKNLGDTLKIILL
                     KDIGESIIYNTTSKFFQEVR"
     gene            2498..3811
                     /gene="aroA"
                     /locus_tag="KPD58_002720"
     CDS             2498..3811
                     /gene="aroA"
                     /locus_tag="KPD58_002720"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBG0266472.1"
                     /translation="MGSLKIYPSKLSGDVKIPPSKSMAHRAVICSSLSNGKSRISNID
                     FSDDIIATIRAMTSLGAIIEKKEDILEISGIFSKEGILNRENQLNQPKLTIDCNESGS
                     TLRFLVPISLAFDGIKRFIGRGNLGKRPLDTYYEIFDRQNIKYSYKENQLDLIISGKL
                     KPDEFRVKGNISSQFITGLLFILPTLESDSKIIITTELESKGYLDLTLSTIKDFGVEI
                     INNNYKEFIIKGNQTYKARDYKVEGDYSQGAFYLSADAIGEDISILDLKEDSLQGDSE
                     VVEILSRMGMEILREGNKIKGITNGLNGTLIDASQCPDIIPVLSVVASLSIGRTTIIN
                     AGRLRIKECDRLHAINVELSKLGANIEEKEDSLIIEGVSKLNGGVEVWSHKDHRIAMT
                     LAIASCRCDKPIILKDFECVSKSYPHFFKDFKMLGGRIDEWNMGK"
     gene            3789..4868
                     /gene="aroC"
                     /locus_tag="KPD58_002721"
     CDS             3789..4868
                     /gene="aroC"
                     /locus_tag="KPD58_002721"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBG0266473.1"
                     /translation="MSGIWGNNLKVSIFGESHGNAIGINIDGLPSGIELDLDNIDKEM
                     KRRAPGKNSISTSRNESDIPEILSGYFNGRTTGTPLCAIIRNSDTRSKDYGELKNLMR
                     PGHADFTGNVRYSGFNDYRGGGHFSGRITAPLVFCGAICKQILSQKGIEIGAHIKKIK
                     NIEDMSFDYVNISKQQLSNLQTLELPLLDLSKEEAMKNTIIDAKNQGDSVGGIIECAV
                     VGINVGLGNPFFDSVESTLSHLLFSVPAVKGVEFGLGFELADMYGSQSNDEMYYEGNQ
                     VKSKTNNNGGIIGGITTGMPIIFKVAIKPTPSISRQQNTVNIKDKKDDILYIKGRHDP
                     CIVQRAIPVIEAVTAIGIFDLMKGR"
     gene            4871..6067
                     /gene="pheA"
                     /locus_tag="KPD58_002722"
     CDS             4871..6067
                     /gene="pheA"
                     /locus_tag="KPD58_002722"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HBG0266474.1"
                     /translation="MEDLTRYRNEIDNIDKELIQLFEKRMNTVLEIARHKMKNNTTIL
                     QKDREEKVLSRAVDNLDNKAYSQETVQFFNSIMEISRGLQKRLIDNDGEQKIEFSLDS
                     SNKKFDLSNVNRYKSLKDELNKKNILVGFSGKSGSFTEEALNKFFNKKISKKQFKEFE
                     DVFIALKNKEINYGIIPIENSSTGAISETYDLLRKYGFYIVGEECIKIDQNLIGIKDT
                     KLEDIKEIYSHPQGIGQCSEFLKQNSTWKLIPFNNTATSAELVRKLQDKTKAAIASKK
                     AASIYGLEIISPCINDIANNYTRFVVISNQIHIEEESNKMSVVFSVEHEAGKLYKVLG
                     YFAKNNINMTKIESRPMKNASWRYFFYIDFECSIYNSKVYDLLELIEHNTAYFKFMGV
                     YKNKTE"
     gene            6114..6923
                     /gene="aroE"
                     /locus_tag="KPD58_002723"
     CDS             6114..6923
                     /gene="aroE"
                     /locus_tag="KPD58_002723"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBG0266475.1"
                     /translation="MNFFGLVGEKLSHSVSPQIHKRVFEILNIESAYKNFEISKEDIS
                     KLDGAIKLLGIQGVNVTVPYKERIMKYLDFISPEAKRIGAVNTILLRENMLYGYNTDY
                     FGLDSMFKMANIDVQGKVAVILGTGGASKAALTYFIDSGIEKLYVSTRKKDDKKLLNS
                     KAILIDYEELKHIKGDIILNATPVGMYPNVGISPVSKSIIQNFDILIDLIYNPGETEF
                     LRIGNSMGKKTCDGLYMLVGQAIKSQEIWQDTKIDNSILDVIYNELKLEFL"
     gene            6935..7468
                     /locus_tag="KPD58_002724"
     CDS             6935..7468
                     /locus_tag="KPD58_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HBG0266476.1"
                     /translation="MINKTKEKLILIGMPGSGKTTIGKLLAKEYNCSFCDMDDYIIQI
                     SQKSIAELFSEGEDIFRNYETQACRELSISDKTVISTGGGVIKKDVNMEILKETGIII
                     FIDRPIQKILEDININSRPLLKNGKDRLYNLYNERINLYKKFSDIEILNDKSLNNAVY
                     NITNAVSENFKFDFKEK"
     gene            7489..>8325
                     /locus_tag="KPD58_002725"
     CDS             7489..>8325
                     /locus_tag="KPD58_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HBG0266477.1"
                     /translation="MNIVIVGLGVIGGSFAKALKKAGYENVFGVDVDLETLKKAEKSK
                     IIKKGCTTGKELFKKADLIILSIYPRLVVDFLNNNKNFFKKGTIITDTTGIKETLIND
                     VLQIIPDDIDFIFGHPMAGREKKGIDFASEQVFNGANYIITPTGRNNIKNLELVENLI
                     LEIGFKRVKKLTSQKHDEIIAFTSQLPHVMAVALINSDEEGRDTGKFIGDSYRDLTRI
                     ANMNEDLWSELFLGNRDNLLKVIENFESEVNLVKEAIFNNDKNKLIEYFKKSSIRREA
                     LEK"
BASE COUNT         3416 a          828 c         1507 g         2574 t
ORIGIN      
        1 taaaaaaata ttttaaattg aaaatattta tagaaaatat tgacaattat attttctttg
       61 ttataattat taaacaagaa gaataataaa ttataaaaat ggaggataat actatgaaag
      121 gaatattaaa agaactaaca gcaaaacttt atagcttttt ttactttatg ttctggggct
      181 tttattttag cgctggctat ttcttttaca aaaatatatg tacttccttt ctggtgaggc
      241 cttattttag tgattttgag cataagtaaa ttttataaaa atggttattt tatatattta
      301 tataagattt agtgcttgat agattatttt agagaactca ttagtgagtt ctctttttta
      361 ttttaaatat aaaattataa tattgggagg tagtaaaaat gatagtagta ttaaaaatgg
      421 gagcagacaa aaatgaagtt aaaaaattaa ttgaggctat tgggagagaa ggagtagaag
      481 ttaatcccat agatggaaca gaattaactg ttttaggttt agttggagat actagtaaaa
      541 tagatgccaa aagaatagaa gcaaataagg tagttgaaaa agttatgcat gtggtagagc
      601 cttttaaaaa ggccaataga aaatttcatc cagaaccttc aatcataaat gttaatggaa
      661 tggaaattgg ttcaaagaaa attgctatga tagctgggcc ttgttctgtt gaaacagaag
      721 accaaatagt atctatagct aaagatgtga aaaaatcagg tgcaggattt ttgagaggtg
      781 gagcattcaa accaagaaca tcaccatacg ctttccaagg attgaaatat gatggattgg
      841 atttattaaa aaaagcaaaa gaaaaaacag gacttcctat agttacagaa ataatgtcaa
      901 cacaagatat agatatattt gaagaaaatg tagatgtaat tcaagttgga gctagaaaca
      961 tgcaaaactt tgatttatta aaagaattag gtaaaacaaa taaaacaata cttttaaaaa
     1021 gaggattatc tgctacaatt gaagagtggc taatgtcagc agagtatata atggctggag
     1081 gaaatgaaaa tgtagttctt tgtgaaagag gtataagaac ttttgaaaca tatactagaa
     1141 atactttgga tttaagtgca attttagctg tgaagaaact tagtcattta ccagttatag
     1201 ttgacccaag tcatgcagca ggtaaatcat ggatggttga ctctttatct aaggcagcaa
     1261 ttgcagttgg agcagatggt ctaataatag aagttcataa tgacccagca catgcacttt
     1321 gtgatggaaa acaatcgatt aagccaaatg aatatgatga attgataagt gaattaaaga
     1381 ctatcgcatc agcagttggt agagaaatat aatctataaa aggatggtta aacgtggaag
     1441 aaaaactatt agtaaattta aaagaaaata gctacaatat atttattaaa aaaaatatac
     1501 taaagaatat aggagaagaa ataaaaaaaa tatattttgg aaataagatt tttattgtaa
     1561 ctgatgaaaa tatagcaaaa ttatacttgg ataaagtaaa aaataattta agtgaatctg
     1621 gatttgaaat taaatcttta atattaaaat ctggtgaaga aacaaaatca tttaatactc
     1681 ttcctaaaat atacgatagt ttacttgact ttaagttaac tagaaaagat ttgattatta
     1741 ctttaggagg aggggtaata ggtgatttag gtggatttgc agcagcaact tttttaagag
     1801 gtataagttt tattcaaatt ccaacatctt tattagcaca agttgatagt tctgtaggtg
     1861 gaaaagttgg agttgacctt gagagaggca aaaatttagt gggaagcttt taccaaccaa
     1921 aggctgtgtt tatcgaccca aatgtattat tatcacttcc agaaaaattc tatagagatg
     1981 gaatggcaga agttataaaa tatggatgta taaaagacaa aacttttttc gataaacttt
     2041 taagtttaaa aaatagagaa gaagtaatgc aacatataga atacattata cataaatgtt
     2101 gctttattaa aaaaactgta gttgaagctg atgaaaaaga ctttggagat agaatgcttc
     2161 taaactttgg ccatacatta ggtcatgcta ttgagaaata ctacaatttt acgggttata
     2221 cccatggaga agctgttgcc ataggaatgt ataagataac tttagaaagt gaaaagatag
     2281 gaattacaaa aaaagggaca tcagaactta taaaatcaat tttaatgaat tataatttgc
     2341 cttatgagat tgaattaaga gataataaat ctatagtaga tgctatatct cttgataaaa
     2401 aaaatctagg tgatacatta aaaataatac ttttaaaaga tattggagag agtattatat
     2461 ataatactac ttctaaattt tttcaggagg taagataatg ggaagtctaa aaatatatcc
     2521 aagtaaatta tctggagatg taaaaatacc accatcaaaa agtatggcac atagggcagt
     2581 tatttgttca tctctaagca atggaaaaag tagaatttct aatatagatt tttctgatga
     2641 tataatagct actataagag caatgacatc attaggagct attatagaaa agaaagaaga
     2701 tattttagaa atttcgggga ttttttctaa agaaggtatt ttaaatagag agaatcagtt
     2761 gaatcaacct aaattgacaa tagattgtaa tgaatcaggc tctacattga gatttttagt
     2821 acctatatct ttagcatttg atggtataaa aaggtttata ggtagaggta atcttggaaa
     2881 aagaccactt gatacttatt atgagatatt tgacagacag aatataaaat atagttacaa
     2941 agaaaatcag ttagatttga ttatatcagg taagcttaaa ccagatgaat ttagagtaaa
     3001 aggaaatata agttctcagt ttatcacagg tctactgttt atattgccta cgctagagag
     3061 tgattcaaag ataataatta ctactgagct agaatctaaa ggatatttag atttaactct
     3121 ttcaacgata aaagattttg gggtagaaat tataaacaat aactataaag aatttattat
     3181 aaaaggaaat caaacgtata aagcgagaga ttataaagta gaaggagatt attctcaagg
     3241 agcattttat ttatcagcag atgctatagg agaagatatt agcatattag accttaaaga
     3301 agattctctt caaggggata gtgaagtagt tgaaatttta tctaggatgg gaatggaaat
     3361 attaagagaa ggcaacaaaa taaaaggaat aacaaatggt ttaaatggta ctttgataga
     3421 tgcatctcaa tgcccagata taatacctgt attatctgta gtagcatcct taagtatagg
     3481 gaggactaca ataattaatg ctggtaggct tagaattaaa gaatgtgata gacttcatgc
     3541 aataaatgtt gagttaagta aacttggagc aaatatagag gagaaagagg atagtttgat
     3601 aatcgaagga gtaagcaagt taaatggtgg agtagaggtg tggagtcaca aagaccatag
     3661 aattgctatg acacttgcaa ttgcttcttg tagatgtgat aaaccaataa tattaaaaga
     3721 ttttgagtgt gtatcaaaat cttatccaca tttttttaaa gattttaaga tgttaggagg
     3781 tcgaattgat gagtggaata tggggaaata atttaaaagt atcaattttt ggagaatctc
     3841 atggaaatgc tataggtata aatatagatg gtcttccttc ggggattgaa cttgaccttg
     3901 ataatattga taaagaaatg aagagaagag cacctggaaa aaattctata tctacttcaa
     3961 gaaatgaaag tgatatacca gaaattctaa gtggatattt taatggaaga actacaggaa
     4021 cgccattatg tgcaatcata agaaatagtg atactcgttc aaaagattat ggagagctta
     4081 aaaatttaat gagaccagga catgcagatt tcacaggtaa tgtgagatat tctggattta
     4141 atgactatag agggggaggt catttttcag gtaggataac agctccatta gtattttgtg
     4201 gggctatatg taaacaaatt ttatcacaaa aaggaataga gataggagca cacataaaaa
     4261 aaattaaaaa tatagaagat atgagttttg attatgtaaa tatttcaaaa caacagttat
     4321 caaatttgca aactttggaa ctacctttac ttgatttatc aaaggaagaa gccatgaaaa
     4381 atacgattat agatgcaaaa aatcaaggag attcagttgg tggtataatt gagtgtgcag
     4441 tagtaggtat taatgtaggg cttggaaatc cattttttga ctctgttgaa tctactttat
     4501 cacatttatt gttttctgtt cctgctgtaa aaggtgtaga gtttggttta ggttttgaac
     4561 ttgcagatat gtatgggtct caaagtaatg atgaaatgta ttatgaagga aatcaagtta
     4621 agagcaaaac aaataacaat ggaggaatta taggagggat tactactgga atgccaataa
     4681 ttttcaaagt ggcaattaag ccaactccat caattagcag acaacaaaac acagttaata
     4741 taaaagataa aaaggacgat attttatata ttaaaggtag acatgaccct tgtatagttc
     4801 aaagggctat acctgtaata gaagcagtaa cagccattgg aatttttgat ttgatgaaag
     4861 gacgataata atggaggatt taacgagata tagaaatgag attgacaata tagataaaga
     4921 gcttattcaa ttatttgaaa agagaatgaa tacagttctt gaaattgcca gacataaaat
     4981 gaaaaataac actactatac ttcaaaaaga tagagaagaa aaagtactaa gtagagctgt
     5041 agataattta gataataaag catattcaca agaaacggta cagtttttta attctataat
     5101 ggaaattagt agaggattac aaaaaagatt gatagataat gatggtgagc agaaaatcga
     5161 gtttagttta gattcatcaa ataagaaatt tgatttgtca aatgtaaata gatataagtc
     5221 tttaaaagat gaattaaata aaaaaaatat cctagtaggg ttttcaggaa aatcaggttc
     5281 atttacagaa gaagcactaa ataaattctt caacaaaaaa atatcaaaaa aacaatttaa
     5341 agaatttgaa gatgttttca tagcacttaa aaataaagaa ataaactatg gaataatccc
     5401 catagaaaat tcatctacag gagctatctc tgaaacttat gaccttttga gaaagtacgg
     5461 tttttatata gttggagaag aatgtataaa aatagaccaa aatttaatag ggataaaaga
     5521 cacaaagctt gaagatataa aagaaatata ttctcatcca caaggtatag gacaatgtag
     5581 tgaattttta aaacaaaata gtacatggaa attgatacca tttaataaca cagcaacaag
     5641 tgcagaactt gtaaggaaac ttcaagataa aacaaaagca gcaattgcaa gcaaaaaagc
     5701 tgcaagtatt tatggattag aaataataag tccttgtata aatgatattg caaacaacta
     5761 tacaagattt gtagtaatca gtaatcaaat acacattgaa gaagaatcaa acaaaatgag
     5821 tgtagtattt tctgttgaac atgaagcagg aaaactttat aaagtacttg gatactttgc
     5881 taaaaataat ataaatatga caaaaataga atcaagacct atgaaaaatg cctcatggag
     5941 atatttcttt tatatagatt ttgagtgttc tatatacaat tctaaagtgt acgatttatt
     6001 agaattgata gaacataata cagcatattt taaatttatg ggagtatata aaaataaaac
     6061 tgaatagata agataaattt ttatttaatt tataattatt aggaggtgta agtatgaatt
     6121 ttttcggtct agtaggtgaa aaactctctc acagtgtttc acctcaaatc cacaagagag
     6181 tattcgagat attgaatatt gaaagtgctt ataaaaactt tgaaatttca aaagaagata
     6241 tatctaagct agacggtgct ataaaattat taggaataca aggagtaaat gttactgtac
     6301 catataagga gcgtattatg aaatatcttg attttatatc accagaggca aaacgtatag
     6361 gagcagttaa tacaatttta ctcagagaaa atatgttata tggttacaat acagattact
     6421 ttggtcttga tagtatgttt aaaatggcta atattgatgt tcaaggaaaa gtagcagtta
     6481 tattaggaac tggaggtgct tctaaagctg ctcttacata ttttattgat tctggtatag
     6541 aaaagcttta tgtttcaact agaaaaaagg atgataaaaa attattaaat agtaaagcta
     6601 tattaataga ttatgaagaa cttaaacata taaaaggaga tataatatta aatgctacac
     6661 cagtaggtat gtaccctaat gtaggaattt caccagtgtc aaaatctatt atacaaaatt
     6721 ttgatatatt aatagattta atttacaatc ctggagaaac agagttttta agaattggta
     6781 atagtatggg taaaaaaact tgtgatggat tatatatgct ggtggggcaa gcaataaaat
     6841 ctcaggaaat ttggcaagat actaaaatag ataatagtat tttagatgtt atatataatg
     6901 aactaaaatt agaatttttg taagggagat tgttatgata aataaaacaa aagaaaaatt
     6961 gatattaata ggtatgccag gtagtggaaa aactacaatt ggtaaacttt tggccaaaga
     7021 atataattgt agtttctgtg atatggatga ttatataatt caaatatctc aaaaaagtat
     7081 agcagagttg ttttcagaag gagaagatat ctttaggaac tatgagactc aagcatgtag
     7141 agaactttct ataagtgata aaacagtgat atctacaggt ggaggtgtaa taaaaaagga
     7201 tgttaatatg gaaatactaa aagaaacagg tataataatt tttattgata gacctataca
     7261 aaagattttg gaagatataa atattaattc aagaccatta ttaaaaaacg gaaaagatag
     7321 attgtataat ctttacaatg agagaataaa tctttataaa aaattttcag atatagagat
     7381 tttaaatgat aaatcattaa ataatgctgt atataatatt acaaatgcag tcagtgaaaa
     7441 ttttaaattt gactttaaag aaaaataatc tagggggaag taaaaataat gaatattgtt
     7501 attgtaggac taggagttat aggaggctct tttgctaaag cattaaaaaa agctggatat
     7561 gaaaatgtat ttggagtaga tgtagattta gaaactttaa aaaaagcaga aaaatcaaaa
     7621 atcataaaaa aaggatgtac tacaggtaaa gagttattta aaaaagctga tttaattata
     7681 ctatctatat atcctagatt agttgtagat tttctaaata ataataaaaa cttttttaaa
     7741 aaagggacta taattactga tactacagga ataaaagaaa cacttatcaa tgatgtgttg
     7801 caaattattc cagatgatat agattttata tttggacatc caatggcagg tagggaaaaa
     7861 aagggtattg attttgcaag tgaacaagtt tttaatggcg caaattatat aattactcca
     7921 actggcagaa acaacataaa aaatttagag ttagttgaaa atttaatttt agagattgga
     7981 tttaaaagag taaaaaagtt aacttcacaa aaacatgatg agattatagc ttttacttct
     8041 cagttacctc atgttatggc agttgctctt ataaatagtg atgaagaagg tagagatacg
     8101 ggtaaattta ttggagatag ttatagagat ttaactagaa ttgcaaatat gaatgaagac
     8161 ttatggagtg aacttttttt aggaaataga gataatttac taaaagttat agaaaacttt
     8221 gaatcagaag taaatttagt aaaagaagct atatttaaca atgataaaaa taaattaata
     8281 gagtatttta aaaaatcatc aataagaaga gaagctttag aaaaa
//
LOCUS       DAELLV010000163         8265 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.163, whole genome shotgun sequence.
ACCESSION   DAELLV010000163 DAELLV010000000
VERSION     DAELLV010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8265
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.163"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            234..1697
                     /locus_tag="KPD58_002726"
     CDS             234..1697
                     /locus_tag="KPD58_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HBG0266478.1"
                     /translation="MTDLVNTINGIVWSPVLVAMCLCIGLYFSLKLKFFQIRDVKEMF
                     TLLLEGDSSHVGISSFQGFATSMAGRIGTGNIAGVAVAIAMGGPGALVWMCIMATLGS
                     ATAFVESTLAQIYKDEHDGQYRGGPPYYFEKGLGWKKCSVLFSIVAIISYTFFMPGTQ
                     SNTLYLAMNQAFGFSKMTIAVASSIALAIIIFGGIKRIGLFAEKVVPIMGGAYLIMTL
                     VIIITNISLVPEAIVTMFKSAFGFGSVFGGMLGSAISWGVKRGIYSNEAGEGSGTYGA
                     AAAEVSHPAKQGLVQAFSVYVDTLLICMATGIMIVITGMYNVTGVCTALPGVEPGPQY
                     VQSAINTLHTQLGDIFIAIVLLLFTFTTLLAFSYMMETNVSYLVTRIKAEKHEKYIIN
                     VCRFMLIACAAMCCFIEPLTSWALGDICIGVLTYINLIAIFTLKKPAIKAYYDYIRQK
                     KMGIKRSDRTFNPIELDIRNAEFWENRYLKEDKHELN"
     gene            1753..2889
                     /locus_tag="KPD58_002727"
     CDS             1753..2889
                     /locus_tag="KPD58_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG0266479.1"
                     /translation="MKERIIQVADSKKEKILGLCQSLYDEPEIALQEYKSAKKISEFL
                     REEGFDVEENLAGMATAFKATKKNGDGPKIAFIAEYDALPGNGHACGHHLIASMGVGA
                     GIALSSILDTYKGEVSIIGTPAEETGDGKPYLIEHGVFDGYDAAMMIHPNSKTCVTPE
                     IIAIGGLDFIFTGKASHAGAKPYNGINALDAVVLLYNNINALRQQLVDGTRIHGIILE
                     AGTAANVIPDMGKVRLEIRAKEQNYFDEVVEKVKNCARGAAIATGCELEFYHFEPTCQ
                     GLNENKVLVDIFTKIMEEFGIYEDEQVMLGSTDMGNLSQIMPCIHPLMKFSENGEELH
                     TKEFLEASINSYAKDRVIDGIKILALTGFNLFENPELLKKMKEE"
     gene            3125..4240
                     /locus_tag="KPD58_002728"
     CDS             3125..4240
                     /locus_tag="KPD58_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HBG0266480.1"
                     /translation="METLEVRNIELSGTNYEIGYRLGELVANMPEIIEGQINKSNLVS
                     KKEEKEMIELFDKYCPGLNEELQGFADAIQVNCNQILYYTMTYLKPGCSQVALAPELT
                     ENGHVLFARNFDFSHNMEDFVLCKTKVNGKYAHIGTTIMQFGRGEGMNECGLGVSQSS
                     CGIPVGNSDGLRKPAIVGLQFWAVIRYLLENCKDVDEALEYLKDMPIAYNINLLLADK
                     SGNIALVETLDGKKEVNIINSLESKREYFLHSTNHTHIDKLHKLDPQSMKNSIHRYKL
                     IKEYINKSKKIGEKELMNLLSSKYPNGLSCNYYNDFFGTLKSIVMDLNIGKFNILWGG
                     LENKWESYYLKNDIKSITQRININIEKAPSDFFDFIE"
     gene            4392..5261
                     /locus_tag="KPD58_002729"
     CDS             4392..5261
                     /locus_tag="KPD58_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG0266481.1"
                     /translation="MDYLKYFEKAVMYIENNLHENITVNDVAKETGYSYYHLTRLFKS
                     MFGESVGSYIKKRRLVSSTKELLYSDKKVIDIAIGSGFESSEAFSRAFKSIYKVSPIE
                     YRKNRIDVFVGKKKKLELDFMRHLVGNITIKPVIKEIEKIKVIGIKDKVILENNSLPD
                     LWEKFRKVHHIVPNTLPSKRVFGICEAISEIHLVSESMEFNEIIGLEVNSYDVIPNSF
                     VSKTIKEGKYAIFTHTGSLDNLDKTYEYIWGTWLLSSKEELDIRDDFEVYDERFLGPS
                     NVNSQIDIYIPIK"
     gene            5637..6182
                     /locus_tag="KPD58_002730"
     CDS             5637..6182
                     /locus_tag="KPD58_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBG0266482.1"
                     /translation="MENVITGLSKGAEWFIGLFQKGGEQFIGLVSGTLPTLIVLMVAI
                     NSLIKIIGEERVNNWASKLGKNSFTRYILLPLVSVFFLGNPMCYTFGRFLKEEHKAGF
                     YDAAVSFVHPITGLFPHANAGELFVWLGISAGLTTLGKETTTLALWYFIVGLIVIFIR
                     GIVTEKMYAFLTRKNRATNKA"
     gene            6195..7208
                     /locus_tag="KPD58_002731"
     CDS             6195..7208
                     /locus_tag="KPD58_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBG0266483.1"
                     /translation="MEENKILKIEKGTSGWGGPLYIKKEGNRNKILSMTAGGIHEVTL
                     KIKELLGCEIVDGFKTGVSDEEVAVVIIDCGGTARCGVYPKKKIPTINVNPVGKTGPL
                     AKFITEEYYVSDVNPNCISVVDGEDMPQKSQENKSENKSSIRKPDNYDEVKSKAQGEY
                     AKKNIILSIGQGAGQVVSKFYDAGRDTIQMVMNNVIPFMAFVSMLMGIILASGLGDWI
                     ARVISPLAGNIGGLLIISVICTLPFLSPILGPGAVIAQVVGTLVGTQIGLGAIPAYLA
                     LPALFAINGQAGCDFVPVGLSLGEAEPETVEYGVPALFYSRLITGPIAVIIAYGVAVF
                     ALR"
     gene            7430..>8265
                     /locus_tag="KPD58_002732"
     CDS             7430..>8265
                     /locus_tag="KPD58_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBG0266484.1"
                     /translation="MSVTEKIPSIDRKIFKILIMCSKKEFVSINSIANELNVTTRSVR
                     TYIKQLNKDLGNDIAVIKYIKGQGYKLEIKDEQILNKIIDMNRKNVFSLNSKEDRVEF
                     ILNYLIELDGFITLDSLADEMCVGRTTLVNDFQYVEKVLASYNLNLIKKQNTGMKLNG
                     NELDIRLFILNQLYKNSRKDLSNSKYFKGIKKEEIINLEEKLLTLFKKNNFYVTDEML
                     REVINYIVVLVYRVKEEKKVKDYDVKFDLLKSYDEYFIAREIKHIISGMFECTLNDEE
                     II"
BASE COUNT         3189 a          836 c         1547 g         2693 t
ORIGIN      
        1 attgtttgat tattattcaa acacacgtcg ttaaaaattt tataaattta tgtttagata
       61 tgattaatac tcttgatttt agaaataaat atttgtagat tgtagattaa agataatata
      121 aataaataga aggtagattt tttagatata ttaatataaa gattatgtaa aatattgaaa
      181 tatagctgtt taaattattg tctataaata taaaatgaat ggtggtgagt taagtgaccg
      241 atttagttaa caccattaat ggaattgttt ggtcacccgt attagttgca atgtgtttgt
      301 gtataggatt atatttttca ttgaaattaa aattttttca aataagagat gtaaaagaaa
      361 tgtttactct cttgcttgaa ggagatagtt ctcatgttgg tatatcatca tttcaaggtt
      421 ttgctacatc tatggctgga agaataggga ctggaaatat agctggtgta gctgtagcaa
      481 tagcaatggg aggtccagga gcattagtat ggatgtgcat aatggctaca ttaggttcag
      541 caactgcatt tgtagaatct actctagctc aaatatataa agatgagcat gatgggcagt
      601 atagaggagg acctccatat tattttgaaa aaggtcttgg gtggaaaaag tgttcagttt
      661 tattttcaat agttgcaata ataagttata cattttttat gcctggtaca caatcaaaca
      721 ctttatatct agcaatgaat caagcatttg gattttcaaa aatgacaata gcagtagcat
      781 cttctattgc attagcgata ataatttttg gaggaattaa aaggattggt ttatttgctg
      841 aaaaagtagt acctataatg ggtggagcat accttattat gacattagta ataattataa
      901 caaatattag tttagttcca gaggctattg taacaatgtt taaatctgca tttggatttg
      961 gttcagtttt tggaggaatg ctaggttcag caatatcttg gggagtaaaa agaggtatat
     1021 actcaaatga agcaggagaa ggttctggga cttatggtgc ggcagcagca gaagtaagtc
     1081 atccagccaa acaaggatta gttcaagcat tttctgttta tgtagacaca ttattaatat
     1141 gtatggctac aggtattatg attgtaataa caggaatgta taatgttaca ggtgtttgca
     1201 cagcactacc aggtgtagaa ccgggaccac aatatgtaca gagtgcaata aatacattac
     1261 atacacaatt aggtgatatt tttatagcaa tagttttatt attatttaca tttacaactt
     1321 tattagcctt ctcatatatg atggagacta atgtatctta cttagtaact agaattaaag
     1381 cagaaaaaca tgaaaagtac ataattaatg tctgtagatt tatgcttata gcatgtgcag
     1441 caatgtgttg ttttatagaa ccacttacaa gctgggcatt aggtgatata tgtataggtg
     1501 tattaacata tataaattta attgccatct ttacactaaa aaaaccagct ataaaggcat
     1561 attatgatta tataagacag aagaagatgg gaataaaaag gagtgataga acttttaacc
     1621 ctatagagtt agatataaga aatgctgagt tttgggaaaa tagatactta aaagaggata
     1681 agcatgagtt aaattaaagt tataataaaa tataaaaatt actaaataaa tttagtggga
     1741 ggtaagattg gtatgaaaga aagaataata caagtggctg attcaaagaa agaaaaaatt
     1801 ctagggctat gtcaatcttt atatgatgaa ccagaaattg ctttgcaaga atataaatct
     1861 gctaaaaaaa tatcagaatt tttaagagaa gagggttttg atgttgagga aaacttggca
     1921 ggtatggcta cagcttttaa agcaaccaag aaaaatggag atggtcctaa aatagcattt
     1981 atagcagaat atgatgctct tccaggcaat ggacatgcat gtggacatca tttaattgca
     2041 tctatgggag ttggagctgg aatagctctt tcaagtatac tggatactta caagggagaa
     2101 gtaagtataa ttggaacacc tgctgaagaa actggagatg gaaagcctta cttgatagaa
     2161 catggtgtat ttgatggcta tgatgcagca atgatgatac atcctaattc aaaaacttgt
     2221 gtaacacctg aaattatagc aataggaggt ctagatttca tatttactgg aaaagcatct
     2281 catgcaggag ctaagccata taacggaata aatgctttag atgcagtagt actactatat
     2341 aataatataa atgcactaag acaacaatta gtagatggaa cgagaataca tggtattata
     2401 ctagaagcag gaactgctgc aaatgtaata cctgatatgg gaaaagtaag attagagata
     2461 agggcaaaag aacaaaatta ctttgatgaa gtagtagaaa aagtgaaaaa ttgtgctaga
     2521 ggtgctgcaa tagccactgg atgtgaactt gaattttatc actttgaacc aacttgccaa
     2581 ggattaaatg aaaataaagt tttggtagac atatttacaa agattatgga ggaatttgga
     2641 atatatgaag atgaacaagt aatgttaggt tcaacagata tgggaaattt aagtcaaata
     2701 atgccatgta tacatccttt gatgaagttt tctgaaaatg gagaagaact acatacaaaa
     2761 gaatttttgg aggcttctat aaattcatat gcaaaagata gagtaattga tggtataaaa
     2821 atattagctt taacaggatt taatttattt gaaaatccag agttattgaa aaaaatgaag
     2881 gaagagtaga acttagacta tttttaatta tactctttaa aataagaaga gtgtctcaaa
     2941 aggaactttt tagtttatga gacactcttt tttgtatgaa attaaatata tttttattat
     3001 tttaaacatg aaaaagaggc ttatctgttt ttatatgacc ttttttaact tgtagcacaa
     3061 aatatcatga atgattacaa ttcgaatgat aaaatcgatt ataagatata taggaggggt
     3121 acatgtggaa actttagaag taagaaatat tgaattgagt ggtacaaatt atgagatagg
     3181 atacagacta ggagaattgg ttgctaatat gccagagata atagaaggtc agattaataa
     3241 atctaatctg gtaagcaaaa aagaagaaaa agagatgata gagttgtttg ataaatattg
     3301 tccaggtctt aatgaagaat tgcaaggttt tgctgatgca attcaagtga attgtaatca
     3361 aattttatat tatactatga cttatcttaa accaggttgt agtcaagttg cattagcacc
     3421 agaattaact gaaaatgggc atgtactatt tgcacgtaat ttcgatttct cacacaatat
     3481 ggaggatttt gttttatgta aaactaaggt aaatggaaaa tatgcacata taggtactac
     3541 cattatgcaa tttggaagag gtgaaggtat gaatgaatgt ggcttaggtg taagtcagtc
     3601 atcatgtggt attcctgtag gcaattcaga tggtttaaga aaaccagcaa ttgtaggttt
     3661 acagttttgg gctgttatta gatatttgtt agaaaattgc aaagatgtag atgaagcatt
     3721 agaatattta aaagatatgc caattgcata caatattaat ttattgcttg cagataagtc
     3781 tggaaacatt gcacttgtgg agactttaga tgggaaaaaa gaagtaaata taatcaacag
     3841 tttagaaagt aaaagagaat attttttaca ctctactaat catacacata ttgataaact
     3901 tcataaatta gatccacaat caatgaaaaa ttctattcat agatataaat taattaaaga
     3961 atatataaat aagtctaaaa aaattggaga gaaagagtta atgaatttat tgtcttcaaa
     4021 atatccaaat ggcttatctt gtaattacta taatgatttt tttggaactt taaaaagtat
     4081 agttatggat ttaaatatag gaaagtttaa tattttatgg ggtggactag agaataaatg
     4141 ggagagctac tatttaaaaa atgacattaa atctataaca cagaggataa acataaatat
     4201 tgaaaaagca ccatctgact tttttgattt cattgaataa aaaatgtatt caattattct
     4261 aaataattag gatatataaa tcaactaaaa gtttttattt atcgtattga attagttata
     4321 atattttagt atagtaaaaa atagtgctat tttacatatt taaagagttt aagtagaaga
     4381 gaggtgccat attggattac ttaaagtatt ttgaaaaagc tgtaatgtat attgagaata
     4441 atttacatga aaatataact gtaaatgatg tggcaaaaga aactggatat tcttattatc
     4501 atttaacacg cttatttaag tctatgtttg gtgagagtgt aggaagttat ataaaaaaaa
     4561 gacgattagt aagtagtaca aaagaacttt tatatagtga taaaaaagtg atagatatag
     4621 ctattggtag tggatttgaa tcttcagaag catttagcag agcatttaaa tctatataca
     4681 aggttagccc tatagaatat agaaaaaata gaattgatgt atttgttggt aaaaagaaaa
     4741 aattagagtt agattttatg agacatcttg ttggaaatat aacgattaaa ccagttataa
     4801 aagaaataga aaaaataaag gtaataggta taaaggataa agtaatttta gaaaataatt
     4861 cattacctga cttgtgggag aaattcagaa aagtacatca tattgtccca aatactttgc
     4921 catcaaagag agtttttgga atctgtgaag caatctctga aatacaccta gttagtgaaa
     4981 gtatggagtt taatgagata ataggacttg aagttaatag ttatgatgtg attccaaatt
     5041 catttgtttc aaaaactatt aaggaaggaa agtatgctat atttactcat actggtagct
     5101 tagataattt agacaaaact tatgaatata tatggggtac ttggctttta agttcaaaag
     5161 aagaacttga tataagagat gattttgaag tatatgatga acgattttta ggtcctagta
     5221 atgtgaactc acaaattgat atttatatac ctataaaata atctataaga aaactaaaag
     5281 ataatttgat taagaaatag atgttcgtaa aagagcatct attttttatt tttatcagat
     5341 ttagataatt ttaataataa aagaaataaa aataagttct taaatttcta agaagaattt
     5401 agaatatagt cgaaataaga attgtatatt tctgaaaatt ctaattagta caattttttc
     5461 ctttataaaa ttgaaaaaag atgtatttat ttatcaaaaa tattatatta aaatgatatt
     5521 aataaaaaaa catttacaat attagcatat agtacagaaa aaagatgtaa tttgtaaaat
     5581 ttatggtaag cagtaaaatt aaaaatttaa agatattata gggaggttat aattttatgg
     5641 aaaacgttat tacaggtcta agtaaaggtg cagagtggtt tatcggtttg tttcagaaag
     5701 ggggagaaca atttataggt cttgtttcag gtactcttcc aacgctaatt gtattaatgg
     5761 tagctattaa ctctttgatt aagataattg gtgaggaaag agttaataat tgggcttcta
     5821 agctaggaaa gaactctttt acaagatata ttctacttcc attagtatca gtatttttct
     5881 tgggaaatcc tatgtgctat acatttggta gatttttaaa agaagagcac aaagcaggat
     5941 tttatgatgc agcagtttca tttgtacatc caattacagg tctttttcca catgcaaatg
     6001 caggagaact ttttgtatgg ttgggaatat cagcaggttt gactacttta ggtaaagaaa
     6061 ctactacact ggcattgtgg tattttatag ttggtctaat tgtaattttt ataagaggta
     6121 tagtaacaga aaaaatgtat gcttttctaa caagaaaaaa tagagcaaca aataaggctt
     6181 aggaggtaga taaaatggag gagaataaaa tattaaaaat agaaaaggga acttcaggct
     6241 ggggaggtcc tttgtatata aaaaaagaag ggaatagaaa taagatacta tcaatgactg
     6301 ctggcggtat ccatgaagta acattaaaaa taaaagaact actgggttgt gaaattgtag
     6361 atggatttaa aactggagtt tctgatgaag aagtagcagt agttattatt gattgtggtg
     6421 gaactgctag atgtggagtt tatccaaaga aaaaaatacc tacaataaat gtaaatccag
     6481 ttggaaaaac aggtccttta gcaaaattta taacagaaga atattatgta tcagatgtta
     6541 atcctaactg tataagtgtt gtagatgggg aagatatgcc acaaaaatct caagaaaata
     6601 aaagcgaaaa taagagttct attagaaagc ctgataatta tgatgaggtt aagtcaaaag
     6661 cacaaggtga atatgctaaa aagaacataa tactttctat tggtcaaggg gcagggcaag
     6721 tagtatcaaa attttatgat gcaggtcgag atactatcca aatggtaatg aataacgtaa
     6781 taccatttat ggcatttgta agtatgctta tgggaattat tttagcatct ggactgggag
     6841 actggattgc aagagttata tctccacttg caggtaatat tggtggatta ttgattatat
     6901 cagtaatttg cacacttcca ttcttatcac caatactagg acctggtgca gttatagctc
     6961 aagtagtagg gactcttgta ggtacacaaa ttggtcttgg tgcaatacca gcatatttag
     7021 cacttccagc tttatttgca ataaatgggc aagctggatg tgattttgta ccagtaggat
     7081 taagtttggg agaagcagaa ccagaaactg tagagtatgg tgttccagct ttattttact
     7141 ctcgtttaat aactggtcca atagctgtta ttattgcata tggagttgca gtatttgcat
     7201 tgagataata tttaatgtca atataaggct tggaattaat taataaaatt gtaaatataa
     7261 atttaagcta gtcaagattt tttgttaatg aaagactagc ttttatacta ttaattagct
     7321 gtaattatcg agttagatta atactaaatt ttcctttatt aaagtgaaat tttatgtatt
     7381 aatttggttt caaatttgta ctagaatgaa atcaaggagg agatacaaaa tgagtgtaac
     7441 tgagaaaatt ccttctattg accgcaagat atttaagata ttgattatgt gtagtaaaaa
     7501 agagtttgtg agtattaact caatagcaaa tgaattgaat gtcacaacta gaagtgtaag
     7561 gacgtatata aaacaattga ataaagattt aggtaatgat atagcagtaa tcaaatatat
     7621 aaagggtcaa ggctataaac tagaaataaa agatgagcag atactcaata agattattga
     7681 tatgaataga aaaaatgtat tttcattaaa ttcaaaagaa gatagagtag agtttatact
     7741 aaattacttg atagaactag atggatttat tacacttgat agtttagctg atgagatgtg
     7801 tgtaggaaga actactttag taaatgattt ccagtatgta gaaaaagttt tggcttcata
     7861 taatttaaac ttgattaaaa aacagaatac aggtatgaaa ttaaatggaa atgaacttga
     7921 tattagactg tttatattaa atcagttgta taaaaactca agaaaagatt taagtaattc
     7981 aaagtatttt aaaggaatca aaaaagaaga aatcataaat ttagaagaaa aactattgac
     8041 tttatttaaa aaaaataatt tttatgttac agatgaaatg ttaagagaag ttattaacta
     8101 catagtagtt ttagtctata gagtaaaaga agaaaagaaa gtaaaagatt atgatgtaaa
     8161 gtttgattta cttaaatcat atgatgaata ctttattgct agggaaataa aacatataat
     8221 atcaggaatg tttgaatgta ctttaaatga tgaagaaatt attta
//
LOCUS       DAELLV010000164         8250 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.164, whole genome shotgun sequence.
ACCESSION   DAELLV010000164 DAELLV010000000
VERSION     DAELLV010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8250
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.164"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            315..650
                     /locus_tag="KPD58_002733"
     CDS             315..650
                     /locus_tag="KPD58_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0266485.1"
                     /translation="MYNIDDMIYNCPVEALSNILGKKWVAIIIWKIQEDKKRFGELQR
                     AIPDCSKKMLVQQLDFLIEQGIIVNQKKLVNNVVESTYFLSESGLRLLPVMEKMIMWS
                     NKNLNCDKY"
     gene            787..2064
                     /locus_tag="KPD58_002734"
     CDS             787..2064
                     /locus_tag="KPD58_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HBG0266486.1"
                     /translation="MQNVNLFLVFIEGIVSFFSPCILPILPIYLSILSNSSVENLKEG
                     KTSFIGSSLFKNTIFFALGISTTFFILGSSVKVLSMFFNENKDLIMFIGGIIIIIMGL
                     FYMGIIKSSILNREKRFNVKFKEMKAITAFILGFTFSFGWTPCIGPILASVLVMVSSS
                     SNHLSANLLIAVYTIGFILPFIITAMFYSKLFKTIDKIKSNMEIIKKIGGIILIVSGI
                     LMMVNGFGSISKHFNTSQNSKIESKQEENKRENSTDKEENSDGNDSQKDSNNDNNDKG
                     SNDEDRIKSIDFTLTDQYGKTHKLSDYEGKVVFLNFWATWCPPCKEEMPYIEQLYKDY
                     NKNNDDVVILGVASPNLGREGSREHVVNFLKEQGYTFPVVLDEDGALAYQYGINAFPT
                     TFIIDKEGYVTQYIPGAMDKATMASFIENQRNK"
     gene            2185..3573
                     /locus_tag="KPD58_002735"
     CDS             2185..3573
                     /locus_tag="KPD58_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG0266487.1"
                     /translation="MKKTFDAIIIGFGKGGKTLAGDLANRGLKVALIEKSNKMYGGTC
                     VNVACIPTKSLENSANSVKTKNINSWDEVQAEYEKAIDKKETLITKLREANYNKLNSN
                     ENVTIFTGMGTFIDEKTVQVKTENEIYELVADNIFINTGSRPFIPNIKGIENKNIVYD
                     SESLMNLRVLPKKMTIIGAGFIGLEFAGIYSSFGAEVTILNSNNGILPNEDVEDSEEI
                     IKLLAKRNVKIVNNANIKEIKEVSELAIVEYEVDGKSKELTSNMILVATGRKANTEGL
                     GLENAGIELNERGFIKVSETLKTNKEHIWAIGDINGGPQFTYISLDDYRIVINQLFGD
                     KTRKTNDRKNIPNSIFISPAFSRVGLNVKQAKEKGYEVLVAKMPVEAIPRAKQIGKAD
                     GFIKIVIDKKSNKILGASMICENSSEIIHLIQLAVDLEVEYTYLRDRVYAHPTMTEAL
                     NDILSPNMIKEV"
     gene            3944..4657
                     /locus_tag="KPD58_002736"
     CDS             3944..4657
                     /locus_tag="KPD58_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017208807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HBG0266488.1"
                     /translation="MNKISKRGFDDSDKRWFSSKELTRLIKAKEEIEWLLNRDYKIDP
                     VVTFVGDRYQFSIRQRDALKRAVCTDEKNIIRQSKRLSLDKIKEDTVNIDGFNLIISI
                     EVGLSGGTLVIGSDGNIRDLAGLRGTYKIIDKTEEALNLIGKFFNKYNAQNLKFYLDA
                     PVSNSGNLKYRILEHAKTWGIETEVELVKNADVVLEKLDRVVSSDAVIVDKCISYFNV
                     ARGIIEEYIKECNIINLNK"
     gene            4775..5644
                     /locus_tag="KPD58_002737"
     CDS             4775..5644
                     /locus_tag="KPD58_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBG0266489.1"
                     /translation="MRGWICVKLTKLNIKKYRAPIYKYALANVNLRSAKSTNSSIITV
                     IPQGAKMEVLDEEDDWIKVMYNSQEGYVYKDLVSVSEYAWSNLNLREDKSTTSNIITV
                     IPEKSRVEVLQVDGDWSKVVYDDKIGYVFNYFLSIDGNKPNELDYKYFYTDMIKFVNE
                     NNIKSTSDYLIVTDLRNKYTYIFKKDNGGWGQLYKWQCTIGKPETPTITGIFYISGRK
                     PSFGTDEYSVKYATRIKGGYYYHSVLYDSTGSYIIDGRLGEALSHGCIRLSTENAKWI
                     YDNIPDTTTVIIH"
     gene            complement(5799..6944)
                     /locus_tag="KPD58_002738"
     CDS             complement(5799..6944)
                     /locus_tag="KPD58_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBG0266490.1"
                     /translation="MSIYYVPPINLLGKGCLNEAKDSIKSLGTKKAFIVSDKFLVSNG
                     TVKKVTDILSQIDIDFVVYDEVKQNPTVTNVNKGLKILKSENCDFVITIGGGSPQDCG
                     KAISILATNGGDIRDYEGINKTSKKCLPIIAITTTAGTSAEVTINYVITDEDRHVKMI
                     MVDTNSLATMTVNDPELMISKPSNLTAATGMDALTHAIEAVVANGAYDVTDSTALYAI
                     KQIFKYLPRAVKNGNDIEAREQMCYACFLNGIAFSNAGLGNVHAMAHQLGGLYDLPHG
                     VCNAMLLPIVEEENAKSAPAKFRPIAEVIGMDVNNKSDKECVDFVIDKIKALSEEVGI
                     PKSLKDVGVDNPNFELLAENSMKDACAGANPVFFDKDKIIELFMKIS"
     gene            complement(7277..8053)
                     /locus_tag="KPD58_002739"
     CDS             complement(7277..8053)
                     /locus_tag="KPD58_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBG0266491.1"
                     /translation="MTSDNHRPTARILDILEALAESENGYTLTEIADVINAPKSSIFP
                     IVHTLNARKYITIDKNTSKYSIGIRSYTLGSSFFEKRTIFNYIIDEMTNIVNSCSETC
                     QLGILDNNEVLYIGKVDSPEPIRLISYVGKKIPANCTGLGKALLCDFDKEKLISLYPD
                     GLKGFTEKSITDFDVLYDQLLEVQRTQIASECEESMEHLKCLAVPIRKNGCIRAAVSV
                     TLPLFRANDEKIELIKKLLLNAQKNIQSMMEERNEDIVIN"
BASE COUNT         3207 a          916 c         1336 g         2791 t
ORIGIN      
        1 ttaacaggat aagcagttta aataaagaat tattatatta gagtgatttt atatataatt
       61 taaaataata aaattttaat aaaatgagtt aaagattgta tattaataga gttgatgtag
      121 ttacaaaaaa gttaccaatt gttatgtatt tacaaaactg atatattaat tacataaaat
      181 ctaaaatagt ttgtaatagg agtatatata ataaaaattt taattaaata aagctaggta
      241 tatttataat attaataaat atattgctat aaaagtttgt ttaataaatt aaataaagaa
      301 aagaggagaa taaaatgtac aacatagatg atatgatata taattgtcca gtagaagctt
      361 tatcaaatat tttaggtaaa aaatgggtgg ctataattat atggaaaata caagaagata
      421 aaaaacgatt tggagagcta caaagagcta taccagactg tagtaagaaa atgttagttc
      481 aacaactaga ttttttaatt gaacagggga ttatagtcaa ccaaaaaaag ttggttaata
      541 atgttgtaga gtcaacatat tttttaagtg aatcaggatt acgattgctt cctgtcatgg
      601 aaaaaatgat tatgtggagc aataaaaatt taaattgtga taaatactaa aatttactct
      661 gtaagtaatg aaaatttaat taagctatga gtagaaaaat gtattaaaat tacataattt
      721 tttagttaga ttttaattta gaaatgggat tgtaatattg gaaataaaat aaggaggaat
      781 tttaatttgc aaaatgtaaa tttattttta gtatttatag aaggtatagt atcattcttt
      841 tcaccatgta tactgccaat tttgcctatt tatttgagta tattgtcaaa tagtagtgta
      901 gaaaatttaa aagaaggaaa gacgagtttt ataggaagtt ctttatttaa aaatactata
      961 ttttttgctc ttggaatctc aacaacattc tttatattgg gttcatcagt aaaagtattg
     1021 agtatgtttt ttaatgaaaa taaagattta ataatgttta ttggtgggat tataattatt
     1081 atcatgggat tattttacat gggaataatt aaatcttcaa tattgaatag agaaaaaaga
     1141 tttaatgtta agtttaaaga aatgaaagca ataacagctt ttatattagg ttttacgttt
     1201 agttttggat ggacaccttg tattggtcca atattggcat ctgtattagt catggtttca
     1261 agttcaagta atcatcttag tgccaatctt ttaatagcag tatatactat tggttttatt
     1321 ttgccattca taattacagc tatgttttac agtaaactat ttaaaactat agataagata
     1381 aaatctaata tggaaattat taaaaaaata ggtgggatta tacttatagt atcaggaata
     1441 ttaatgatgg taaatggatt tggaagtata agcaaacact ttaatacgtc acaaaatagt
     1501 aaaattgaaa gcaagcaaga agaaaataag cgagaaaatt ctactgataa agaagaaaat
     1561 tctgatggta atgatagtca aaaagactct aataatgaca ataatgataa agggtctaat
     1621 gatgaagata gaataaagtc tattgatttt actttaacag accagtatgg taaaacacat
     1681 aaattaagtg attatgaagg caaagttgta ttcttaaatt tctgggctac atggtgtcct
     1741 ccatgtaaag aagaaatgcc ttatatagaa caactgtata aagactataa taaaaataat
     1801 gatgatgtag taattttagg agtagcttca cctaatttag gtagagaagg ttcaagggaa
     1861 catgtagtta acttcttaaa agaacaaggt tatacattcc ctgttgtact tgatgaagat
     1921 ggagcattag catatcaata tggaattaat gcttttccaa ctacatttat aattgacaag
     1981 gaaggatatg taactcagta tattccagga gctatggata aggcaactat ggcatcattt
     2041 attgaaaatc agagaaataa ataaaagtat ttaagtataa gtttataaat caatttaagg
     2101 ttacaaaaaa gtgcctaatt gtaccaccta aataatcttg ttatagtaaa agtataaaca
     2161 atacaagaaa gggttgaatt ttatatgaaa aaaacatttg atgctataat aataggattt
     2221 ggaaaaggtg gaaaaacatt agcaggggat ttggcaaaca ggggattaaa agtagcttta
     2281 atagaaaagt caaacaagat gtatggtgga acatgtgtaa atgtggcatg tataccaaca
     2341 aaaagcttag aaaattcagc aaacagcgta aaaacaaaga atataaattc ttgggatgaa
     2401 gtacaagctg aatatgagaa agctatagat aaaaaagaaa ctttaattac aaaactcaga
     2461 gaagctaact ataataaatt aaattcaaat gagaatgtaa ctatattcac aggaatggga
     2521 actttcattg atgaaaaaac agttcaagtc aaaactgaaa atgaaattta tgagttagta
     2581 gcagataata tatttattaa tactggttca agaccattta tacctaatat taagggaatt
     2641 gaaaataaga atatagtata tgatagtgag tctcttatga atctaagagt tttacctaaa
     2701 aaaatgacaa ttataggagc tggatttata ggtttagaat ttgctggtat ttatagttca
     2761 tttggagcag aagttacaat attaaactct aataatggca ttttaccaaa tgaagatgtt
     2821 gaggattcag aagaaataat aaaattactt gcaaagagaa atgtaaaaat tgttaataat
     2881 gcaaatataa aagagattaa agaagtttct gaattagcaa tagtagaata tgaagtagat
     2941 ggtaaatcta aagaattaac aagtaatatg atacttgtag ctacaggaag aaaagcaaac
     3001 actgaagggc tagggctaga aaatgcaggt atagaattaa atgaaagagg atttattaaa
     3061 gtctcagaaa ctcttaagac taataaagag catatatggg ctataggaga tataaatgga
     3121 ggtccacaat ttacttatat ttcattggat gattatcgca tagttattaa tcagttattt
     3181 ggagataaga ctagaaaaac taatgataga aagaatatcc caaattctat atttataagt
     3241 ccagcatttt caagagttgg tcttaatgtt aagcaagcta aagaaaaggg atatgaagta
     3301 ttagtggcaa agatgccagt agaagcaatt ccaagagcta aacagattgg caaggcagat
     3361 gggtttataa aaatagtgat agataaaaaa tccaataaga tacttggagc tagtatgata
     3421 tgtgaaaatt caagtgagat tatccatctt attcaattag ctgtagattt agaagtagag
     3481 tatacatatt taagagaccg tgtttatgca catccaacta tgacagaagc tcttaatgat
     3541 attctatctc ctaatatgat aaaagaagta taacataagt ttaatatata aacctaaaat
     3601 tatcattata agtagatttt agaaagagac tatctcaaaa aatagagata agtctctttt
     3661 ttactgttta aataataaaa atatatttga ttttataaaa gaagattatt ttatgagcta
     3721 aaaagtttat ttagaaacac tacctattta tgttataaag ttataaatat aatatagaat
     3781 ctattattct gtattattaa gtggggttaa gcgttataat taattataaa tattgaataa
     3841 tctaaatgga taagataatg gtattaaaaa ttatcttaaa aggtgaaata atctgtattt
     3901 aatgtagtac agttatatct ttaaagcaag gtggtgaaat gatataaata agatatctaa
     3961 aagagggttt gatgatagtg ataaaagatg gttttcaagt aaagaattaa caagacttat
     4021 aaaggctaaa gaagaaatag aatggttatt aaatagggat tacaaaatag acccagtcgt
     4081 tacttttgtt ggtgatagat atcagttttc tataagacaa agagatgcat taaaaagagc
     4141 agtctgtact gatgaaaaaa atattataag acaatcaaag aggctttctc tagataaaat
     4201 caaagaagat accgtaaata tagatggctt taatttgata atatccatag aggtaggatt
     4261 atctggtggt actctagtaa taggaagtga tggcaatata agagatttag caggtcttag
     4321 gggaacctat aagattatag ataaaacaga agaagctttg aatctgatag gtaaattttt
     4381 taataaatac aatgcacaga atcttaaatt ttatttagat gctccagttt ctaattcagg
     4441 aaatttaaag tataggatat tagagcatgc caaaacttgg ggaattgaaa ctgaagtaga
     4501 attagtaaaa aatgcagatg ttgtattgga aaaattagat agagtagtta gtagtgatgc
     4561 agtcatagtt gacaagtgta taagttattt taatgtggct agaggtataa ttgaagagta
     4621 tattaaagaa tgtaatatca taaatttaaa taaatagata aatataggtg agaatatata
     4681 agtatatgta gcaatattct tagtaataca agtaaagttt gaaaatagga gaacgaatac
     4741 atatcgaatt tcataaaatg ttgtaaaaag acatatgaga ggatggatat gtgtgaaatt
     4801 aactaaacta aatattaaaa aatatagagc acctatatat aaatatgctt tagcaaatgt
     4861 aaatttgagg tctgctaaat ctactaattc aagtatcata acagtaatac ctcagggagc
     4921 caaaatggaa gtattggatg aagaagatga ctggattaag gtgatgtaca attctcaaga
     4981 aggatatgtg tataaggatt tggtatcagt aagcgaatat gcttggagta acttaaactt
     5041 aagagaagat aaatctacaa cttccaatat aattacagta attcctgaaa aatctagggt
     5101 tgaagtcctt caagtagatg gagattggag taaggttgta tatgatgata aaataggata
     5161 tgtttttaat tattttttat caattgatgg gaataaacca aatgaattag attataagta
     5221 tttttatact gacatgatta aatttgttaa cgaaaataat attaaaagta ctagcgatta
     5281 tctaattgta acagacctta gaaataaata tacttatatt tttaagaaag ataatggagg
     5341 ttggggacaa ctttacaagt ggcaatgtac tataggcaag cctgagactc caactataac
     5401 aggtatattt tatataagtg gaagaaaacc ttcttttgga acggatgagt attcagttaa
     5461 gtatgctact agaattaaag gtggatatta ctatcattct gtactatatg attcaacagg
     5521 aagttatata atagatggca gacttggtga agctcttagt catggatgta taagattaag
     5581 tacggaaaat gcaaagtgga tatatgataa tataccagat acaacaacag ttataattca
     5641 ttaagaaaaa ttttgagttt tttattggga taatgattct taaattaata ggattatata
     5701 ttaacttctc ataaagagta ctatttaaaa agtgtaataa gagagtgcta ttaacaaaac
     5761 aaagatattt tgaggcactc tcttataaat atacataatt aagatatttt cataaacaat
     5821 tctataatct tatctttatc aaagaaaaca ggattagcac cagcacaagc atctttcata
     5881 gagttttctg caagcaattc aaaattagga ttatcaacac ctacatcttt taaagatttt
     5941 gggattccaa cctcttcaga aagagctttt atcttatcta ttacaaagtc aacacactct
     6001 ttatctgatt tattatttac atccattcca ataacttcag ctattggtcg gaatttggca
     6061 ggggctgatt tagcattttc ctcctcaact attggaagta gcatggcatt acatactcca
     6121 tgaggtaaat catataaacc tcctaattga tgagccatag catgtacatt tccaagacct
     6181 gcattactaa aggcaattcc atttaaaaaa caagcataac acatttgttc tctagcttct
     6241 atgtcattac catttttaac agctcttgga aggtatttaa atatctgctt tatagcatat
     6301 aaggcagttg aatctgtaac atcataagca ccattagcta ctacagcttc tatagcatga
     6361 gttagtgcat ccataccagt tgctgctgtt aaattagatg gtttgctaat cattaattct
     6421 gggtcattta ctgtcattgt ggctagtgaa ttggtatcaa ccattatcat ctttacatgt
     6481 ctgtcttcat cagttataac atagtttatt gtaacttcag cagatgtacc agcagtagta
     6541 gttatagcta tgataggaag acatttttta gaagttttgt tgataccttc atagtctcta
     6601 atatctcctc cattagtggc taatatagag atagctttac cacagtcttg tggagaacca
     6661 cctcctatag ttataacgaa gtcgcaattt tctgatttta atattttaag tcctttattc
     6721 acattagtta cagttggatt ttgtttaact tcatcataaa caacaaagtc tatatctatc
     6781 tgagataaaa tatctgttac tttttttaca gtaccatttg aaactagaaa tttatcacta
     6841 acaataaatg cttttttagt tcctaatgat ttaattgaat ctttagcttc attaagacat
     6901 cctttcccaa gtaaatttat tggtggtaca taataaatgc tcataactaa aatctccttt
     6961 tattttcatt gatttttatg taaaaatata tatctaacaa ttttcactaa tttactcata
     7021 atatatcgta tcttagattg agatagggtt gctggttctt atataagaac ttaattcgta
     7081 attacgatta tgattagaat ttaacatagt atttttatca tgtcaataaa aatataaaaa
     7141 atattataaa aaaaacaaag ttactgtagt taaatcagca actttgtttt tatagtataa
     7201 atcaaataaa tacattagtt taaaacaaaa tttttaatta ttatgttttg atatcttcca
     7261 tatacagaat attaatctaa tttattacaa tgtcttcatt acgttcttcc atcattgatt
     7321 gaatattttt ctgagcattt aagagtaatt tttttatcag ttctattttt tcgtcattag
     7381 ctctaaataa aggcagtgtt acactaacag cagctcttat acatccattt tttcttattg
     7441 gtacggcaag acattttagg tgttccattg attcctcaca ctctgaagct atttgtgttc
     7501 tttgaacttc aagaagctgg tcgtataaaa catcaaaatc tgtaattgat ttttctgtaa
     7561 aaccttttag accatctgga tataagctta ttagtttttc cttgtcaaaa tcacataaaa
     7621 gagcttttcc aagacctgta caattagcag gtattttttt tccaacataa gaaataagtc
     7681 taatcggttc tggagaatct acttttccaa tataaagaac ttcattattg tcaagtatac
     7741 caagctgaca agtttcagaa caggaattaa ctatattagt catttcgtca attatatagt
     7801 taaaaatagt tcttttttca aaaaatgaag accctaatgt gtaagaacgt atccctatag
     7861 aatatttgga tgtgttttta tcaatagtaa tgtattttct agcattaagt gtatgaacta
     7921 taggaaagat gctacttttt ggagcattga taacatctgc tatttctgta agagtatatc
     7981 cattttcgga ttctgctaat gcttctaaaa tatctaatat cctagctgtt gggcgatgat
     8041 tatctgaagt cataaatttc cccctctttt tattaattta aagtattaga atatgttgat
     8101 tttagcacac tatacgatat tttacaatgt agaaaataaa tatgtaaatt tatatataaa
     8161 taagctcttg atttagatac agatagatat ttttttagac tgtacattaa ggaataacta
     8221 atttccaaaa tatacctaaa gaaagtaagt
//
LOCUS       DAELLV010000165         8089 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.165, whole genome shotgun sequence.
ACCESSION   DAELLV010000165 DAELLV010000000
VERSION     DAELLV010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8089
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.165"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            27..101
                     /locus_tag="KPD58_002740"
     tRNA            27..101
                     /locus_tag="KPD58_002740"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Thr,seq:tgt)
     gene            116..200
                     /locus_tag="KPD58_002741"
     tRNA            116..200
                     /locus_tag="KPD58_002741"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Tyr,seq:gta)
     gene            210..293
                     /locus_tag="KPD58_002742"
     tRNA            210..293
                     /locus_tag="KPD58_002742"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:244..246,aa:Leu,seq:tag)
     gene            317..391
                     /locus_tag="KPD58_002743"
     tRNA            317..391
                     /locus_tag="KPD58_002743"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:349..351,aa:Gly,seq:gcc)
     gene            416..492
                     /locus_tag="KPD58_002744"
     tRNA            416..492
                     /locus_tag="KPD58_002744"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:450..452,aa:Arg,seq:tct)
     gene            502..577
                     /locus_tag="KPD58_002745"
     tRNA            502..577
                     /locus_tag="KPD58_002745"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:535..537,aa:Gln,seq:ttg)
     gene            586..661
                     /locus_tag="KPD58_002746"
     tRNA            586..661
                     /locus_tag="KPD58_002746"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:619..621,aa:Lys,seq:ttt)
     gene            664..752
                     /locus_tag="KPD58_002747"
     tRNA            664..752
                     /locus_tag="KPD58_002747"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:698..700,aa:Ser,seq:tga)
     gene            756..831
                     /locus_tag="KPD58_002748"
     tRNA            756..831
                     /locus_tag="KPD58_002748"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:789..791,aa:Phe,seq:gaa)
     gene            838..911
                     /locus_tag="KPD58_002749"
     tRNA            838..911
                     /locus_tag="KPD58_002749"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Met,seq:cat)
     gene            926..1002
                     /locus_tag="KPD58_002750"
     tRNA            926..1002
                     /locus_tag="KPD58_002750"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:960..962,aa:Ile,seq:cat)
     gene            1020..1096
                     /locus_tag="KPD58_002751"
     tRNA            1020..1096
                     /locus_tag="KPD58_002751"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1054..1056,aa:His,seq:gtg)
     gene            1105..1180
                     /locus_tag="KPD58_002752"
     tRNA            1105..1180
                     /locus_tag="KPD58_002752"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1138..1140,aa:Lys,seq:ttt)
     gene            1188..1261
                     /locus_tag="KPD58_002753"
     tRNA            1188..1261
                     /locus_tag="KPD58_002753"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1220..1222,aa:Cys,seq:gca)
     gene            1269..1345
                     /locus_tag="KPD58_002754"
     tRNA            1269..1345
                     /locus_tag="KPD58_002754"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1303..1305,aa:Arg,seq:acg)
     gene            1358..1433
                     /locus_tag="KPD58_002755"
     tRNA            1358..1433
                     /locus_tag="KPD58_002755"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1391..1393,aa:Val,seq:tac)
     gene            1509..1922
                     /locus_tag="KPD58_002756"
     CDS             1509..1922
                     /locus_tag="KPD58_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266492.1"
                     /translation="MYIVIGNEIVDSEELKIIIDKNSEFKVEKDLSKSTKREDVIAYQ
                     LSIDLNYLDSLINEQCNLASLSDEEKFDEYMTLSDELALDLEELMPKYTIINARAYKL
                     DEVDGIVKIILAVAYADLGHLKLSDVVKRLSRQVD"
     gene            2230..5661
                     /locus_tag="KPD58_002757"
     CDS             2230..5661
                     /locus_tag="KPD58_002757"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HBG0266493.1"
                     /translation="MLKKFNKILVANRGEIAIRIFRACSELGIKSVGIYSKEDKYGLF
                     RTKADESYLIGEGKGPIDAYLDMDGIIDLAKRKKVDAIHPGYGFLAENAEFARKCEEN
                     GITFIGPSSKVMNMMGDKINSKKIAKEVNVQTIPGVEKAIRSTEEAKEVANKIGYPVM
                     IKASNGGGGRGMRIVHREEDLELEYETACSESRKAFGEDIIFIEKYIADPKHIEVQIL
                     GDNYGNIVHLYERDCSVQRRHQKIIEYAPAFSLDDKVRKEICEDAVKLSKHVGYSNAG
                     TLEFLVDANGGHYFIEMNTRVQVEHTVTEMVTGIDIVQSQILIAQGYSLDSEEINIKS
                     QDDVEIRGYSIQCRITTEDPKNKFMPDTGKIQVYRTGSGFGIRLDGGNGFTGANISPH
                     YDSLLVKTISWDRTFQGAINKTIRSIKELRVRGVKTNVGFLVNVLNNPIFSNGKCSTK
                     FIDENPDLFEITESKDRGTKLLQFIGDVIVNDNACKEKPLFDALHDPRMDKDGSKSEG
                     SKILFDKLGKSAYIEKIKNDKKLLLTDTTMRDAHQSLLATRIRTYDLLKAAKPTEKYQ
                     KDLFSLEMWGGATYDVAYRFLKESPWRRLQKLREEIPSIMFQMLLRASNGVGYKNYPD
                     NVIEEFTKESARQGIDVFRIFDSLNWVENMKPSINTALETGKIVEATMCYTGDILDKT
                     KTKYNLEYYIKMAQELESLGADIIAIKDMSGLLKPYSAYTLVKELKKNVKAPIHLHTH
                     DTSGNGVATCLMASEAGVDIIDAALESMAGLTSQPSLNAIVEALKNTERDTGIDLFGY
                     DELGKYYKDLRKVYNKFESDLTNSCAEIYNFEIPGGQYTNLKPQADSLGLVNRFDEVK
                     EKYKEANEVVGDIIKVTPSSKVVGDLAIFMTKNKLDKDNIIEEGKNLSFPDSVVDYCK
                     GMIGQPEGGIPKDLQEVVLKGEEAITVRPGSLLPAEDFDEIAKYLNEKYDINANIRNV
                     ISYALYPKVYEDYIKHLQHYNDISKLESDVFFYGLNKNEECEVEIEEGKVLTIRLVEI
                     GEVKENGFRTIGFELNGMVREVEIKDKNFSGKINNVEKADMNDPLQIGASIPGKVIKI
                     MVKEEDEVKANQPLIVIEAMKMETIIVAKTDGVIKSIKVKEDDMVEDKQLLMIMK"
     gene            5903..7831
                     /locus_tag="KPD58_002758"
     CDS             5903..7831
                     /locus_tag="KPD58_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBG0266494.1"
                     /translation="MKVYDSKMLRNVAVLGHSGCGKTNLIETIAYTANTNKIPKLTDK
                     VNMTYSMGLIPIEYNDYKFNLLDTPGYFDFSGDVVSSLRASDAAIIVIDATAPIQVGT
                     EKSLELTESIPKIMFINKIDNEKARYKDAIAMLREKYNNKIVPMISPIYKDKNFVKLH
                     NVFENIDDLEGEFKEQAMSVKEALMELIAETDDQILDKYFNGEELTTEEIQKGIIIGI
                     QRGDIIPVICGSTINNIGTKEILDTISSYLEPIFTEESKPFRGLVFKTMVDPFVGKMS
                     YIKITEGVLSKDKDVFNINKNVKEKIANIYTLRNSELVEIEKAKAGDIVVITKVNSLK
                     TGDTISADKDAEALEKIDFPKPQIYYAVTPKNKGDEEKVASVLNKLVEEDPTLHWYRN
                     TETKQALLGGQGELHIKTIKNKMKDKFGVDVELNDLKVPYRETIKGTADVQGKHKKQS
                     GGHGQYGDVKIRFERCESDFEFTEEIFGGSVPKQYIPAVEKGLKDSMQKGILAGYPVT
                     NIKATLYDGSYHDVDSSEMAFKMAASAAFKKGMEEAHPILLEPIMKLKITVPEEYMGD
                     VMGDINKRRGKIFGMEPDDKGKQIIFAEAPQAETFKYAIDLRAMTQGRGYFEMELERY
                     GEVPSQFAEKIIGLATAK"
BASE COUNT         3174 a         1022 c         1608 g         2285 t
ORIGIN      
        1 ccccttccag gtcgccaaaa taaaatgctg gtgtggctca acggtagagc agctgacttg
       61 taatcagcag gttgtaggtt cgattcctat caccagctcc atataaaatt aatacggagg
      121 atttcccgag cggccaaagg gggcagactg taaatctgtt gtcatcgact tcggtggttc
      181 gaatccacca tcctccacca aaaaaatgcg cggatgtggc ggaattggca gacgcactag
      241 acttaggatc tagcgccatt ggcgtggggg ttcgactccc ttcatccgca ccatttttat
      301 ttaatttaat aaacgagcgg gaatagttca gtggtagagc gcaaccttgc caaggttgaa
      361 gtcgcgagtt cgaatctcgt ttcccgctcc aaattttatg tttatgttgt aatgtgggtg
      421 catagctcag ctggatagag caacgccctt ctaaggcgtg tgtccggggt tcgaatccct
      481 gtgcgctcac cacatatata ttggggatta gccaagtcgg taaggcacat gactttgact
      541 catgtatgcg taggttcgag tcctgcatcc ccagccaaac agtatggtct attagctcag
      601 tcggtagagc acctgacttt taatcagggt gtcccgcgtt cgagtcgcgg atagatcacc
      661 aatggagagg tgtccgagtg gtttaaggag ctggtcttga aaaccagtga cttcgaaagg
      721 ggccgtgggt tcgaatccca ccctctccgc caaatgccca gatagctcag tcggtagagc
      781 aggggactga aaatccccgt gtcggtggtt cgattccgcc tctgggcacc aaaatgtggc
      841 ggtatagctt agttggctag agcgttcggt tcatacccga aaggtcatag gttcgactcc
      901 tattaccgct actaaaattt aatgtgggcc attagctcag ttggttagag cgcccggctc
      961 ataaccggta ggtctggggt tcgagtccct gatggcccac cacattaaat taacttatgg
     1021 tgggtatagc tcagttggtt agagcgccag attgtggctc tggaggtcgt gagttcgact
     1081 ctcattatcc accccaactt atatggtcta ttagctcagt cggtagagca cctgactttt
     1141 aatcagggtg tcccgcgttc gagtcgcgga tagatcacca aaaaggcggc gacatagcca
     1201 agtggtaagg cagtggactg caactccttg atccccagtt cgaatctggg tgtcgcctcc
     1261 aataaaatgc gctcatagct caactggata gagtgtctga ctacgaatca gaaggttagg
     1321 ggttcgagtc cctttgggcg caccatatga atagcgaggg attatagctc agctgggaga
     1381 gcacctgcct tacaagcagg gggtcacagg ttcgagccct gttaatccca ccagtgcaaa
     1441 cctctagtaa aactactaga ggttttattt ttgattgaaa aagtttttat atttaggagg
     1501 taaaaaattt gtatatagta ataggaaatg aaatagttga tagtgaagaa cttaaaataa
     1561 ttatagataa aaatagtgaa tttaaagttg aaaaagattt aagtaaaagc acaaaaagag
     1621 aagatgtaat agcatatcag ttaagtatag atttaaatta cctagattct cttataaatg
     1681 agcaatgtaa tttagccagt ctatctgatg aagagaaatt tgatgaatat atgactttgt
     1741 ctgatgaact tgcactggat ttagaagagt taatgccaaa atatacaata ataaatgcta
     1801 gagcatataa attagacgag gtagatggaa ttgtaaaaat aatactagct gtagcgtatg
     1861 cagatttagg gcacttaaaa ctttctgatg tagtaaagag gctatctaga caagtagatt
     1921 aggatatgaa tatgaactat attaaaaaaa tgtataaaat cttacgtaaa gatgaaaata
     1981 attatgtaaa catagaaaaa acagtataaa atcaagtcaa ttgtagaaat tttcacataa
     2041 attcatctga aattctataa aaaatgcctt aattttaatg aggtaaatcg agtttacaca
     2101 gtctaaaaat tacttatatt tataaattta tcttattaat taaggaactt tcttaaacat
     2161 cttcaaaaat aaagtatttt agtctataat ctttaatatt acacaaacaa taaaggagaa
     2221 gaatagagta tgcttaaaaa gtttaataaa atactagtgg ctaatagagg cgaaatagct
     2281 ataagaatat ttagagcctg ttcagaattg gggataaaaa gtgttggtat atacagtaag
     2341 gaagataagt atggattgtt tagaacaaaa gcggatgaat catatttgat aggagaaggt
     2401 aaagggccaa tagatgcata tttagatatg gatggaataa tagacctagc aaagagaaaa
     2461 aaagttgatg ctatacatcc aggatatggt tttctagcgg agaatgcaga atttgctcgt
     2521 aaatgtgaag agaatggaat tacttttata ggaccatcat ctaaggtaat gaatatgatg
     2581 ggggataaga ttaactctaa aaaaatcgca aaagaagtta atgttcaaac tataccagga
     2641 gtagaaaaag caataagaag tactgaagaa gcaaaggaag ttgcaaacaa aataggatat
     2701 cctgttatga taaaagcatc taatggcggt ggcggtagag gtatgagaat tgtacatcgc
     2761 gaagaagact tagagcttga atatgaaact gcttgtagtg aatcaagaaa agcttttgga
     2821 gaagatatta tatttataga aaaatatata gctgatccaa aacatataga agttcaaata
     2881 ttaggtgata attatggaaa tatagttcac ttatatgaaa gagattgttc agttcaaaga
     2941 agacatcaaa aaataataga atatgcacca gctttttcgt tagatgataa agtaagaaaa
     3001 gagatatgtg aagatgcagt taagttatca aaacatgtgg gttattctaa tgcgggaaca
     3061 ttagaatttt tagtagatgc aaatggtggg cattatttta tagagatgaa cacaagagta
     3121 caggttgaac atactgtaac agagatggtt acaggaatag atatagttca aagtcaaata
     3181 ctaatagccc aaggttacag cttagatagt gaagaaatta atataaaatc acaagatgat
     3241 gtggaaataa gaggttactc tatccaatgt agaataacta cagaagaccc taagaataaa
     3301 ttcatgcctg atacaggtaa aattcaagtt tatagaactg gttcaggatt tggtataaga
     3361 ctagatggag gaaatggatt tacaggggca aacataagtc ctcattatga tagtttattg
     3421 gttaagacaa tatcttggga tagaacattc caaggagcaa taaataaaac tataagatct
     3481 ataaaagaac ttagagttag aggtgttaaa actaatgtag gattcctagt taatgtttta
     3541 aataatccta tattctcaaa tggtaaatgt agtactaagt ttatagatga aaatccagat
     3601 ttatttgaaa taacagaaag taaagacaga ggaacaaaat tacttcaatt tataggagat
     3661 gtaattgtaa atgataatgc ttgtaaagaa aaaccattat ttgatgcatt acatgaccca
     3721 agaatggata aggatggttc aaaatcagaa ggaagtaaaa tacttttcga taagttaggt
     3781 aaatctgcat atatagaaaa gataaaaaat gataagaaat tactattaac agatacaaca
     3841 atgagagatg cacatcaatc attattagca acaagaataa gaacatatga cttattaaaa
     3901 gctgctaagc caacagaaaa atatcaaaag gatttattct cacttgaaat gtggggagga
     3961 gctacttatg atgttgcata tagattttta aaagaatctc cttggcgtag attacaaaag
     4021 ctaagagaag aaataccaag cataatgttc caaatgttgc ttagagcatc aaatggagtt
     4081 ggatataaaa actatccaga taatgtaata gaagaattta caaaagaatc tgctagacaa
     4141 ggtatagatg tatttagaat atttgattca ttaaactggg ttgaaaatat gaaaccatct
     4201 attaatacag ctttagaaac tggaaaaatt gttgaagcaa ctatgtgtta tacaggtgat
     4261 atactagata agactaaaac taaatacaat ctagaatatt atataaaaat ggcacaagaa
     4321 ttagaaagct tgggagcaga cataatagca ataaaagata tgtcaggtct tcttaaacca
     4381 tattcagctt acacattagt taaagagtta aagaaaaatg taaaagcacc aatacattta
     4441 cacactcatg atacaagtgg aaatggtgta gcaacttgtt taatggcgtc agaagctgga
     4501 gtagacataa ttgatgctgc attagaatct atggcaggac ttacaagtca gccatcactt
     4561 aatgctatag tagaggcact taagaataca gagagagata caggaataga tttatttgga
     4621 tatgatgagt taggaaaata ttataaggat ttaagaaaag tttataataa atttgaaagt
     4681 gatttaacta attcttgtgc agaaatatat aattttgaaa taccaggagg ccaatataca
     4741 aacttaaaac ctcaagctga tagtttagga ttagttaata gatttgatga agttaaagaa
     4801 aaatataaag aagctaatga agttgtagga gatataataa aagtaactcc atcttcaaaa
     4861 gttgtaggag acttagctat ctttatgact aaaaataaat tagataaaga caatataata
     4921 gaagaaggta aaaatttatc attcccggat tctgttgtgg attactgtaa aggtatgatt
     4981 ggtcaaccag aaggtggaat accaaaagat ttacaagagg tagttctaaa aggtgaagaa
     5041 gctataacag taagaccagg ttcattatta ccagcagaag attttgatga aatagctaaa
     5101 tatctaaatg aaaagtatga tataaatgct aatataagaa atgttataag ttatgcttta
     5161 tatccaaaag tatatgaaga ttatatcaaa catctacaac attataatga tatatctaaa
     5221 cttgaaagtg atgtattctt ctatggatta aataaaaatg aagaatgtga agtagaaata
     5281 gaagaaggta aggtattaac aataagatta gttgaaattg gtgaagttaa ggaaaatgga
     5341 tttagaacta taggatttga gttaaatgga atggttagag aagtagaaat aaaagataag
     5401 aatttctctg gaaaaattaa taatgttgaa aaagctgata tgaatgaccc tcttcaaatt
     5461 ggagcaagca taccaggaaa agttataaaa ataatggtta aagaagaaga tgaagtaaaa
     5521 gcaaatcaac cattaatcgt tatagaggct atgaagatgg aaactataat agttgctaag
     5581 actgatggag taattaagtc aatcaaagta aaagaagatg atatggttga agataaacaa
     5641 ttattaatga taatgaagta atttaattaa aataaattaa aaagcactct gaaatgttta
     5701 ataaaaaatg ttttagagtg tttttattta ttttaattaa atatttgtta aaaaaaagag
     5761 gacaattacc tccagatgta gaaataaagc tagtaaggaa aatgttatct aaaaattcta
     5821 aatatagtaa atattttcca taatatttct taggaaataa cagcaaagat aaaatacata
     5881 aatttaaaag gtgggtgctt atatgaaggt ttatgatagt aaaatgttga gaaatgtagc
     5941 ggtattagga catagcggat gtggaaaaac aaatctaatc gaaactatag cttacacagc
     6001 aaacactaat aagataccta aattaacaga caaggtaaat atgacttata gcatgggatt
     6061 aatacctata gagtataatg attataagtt taacttattg gataccccag gatattttga
     6121 ctttagcgga gacgttgtgt catctcttag agcaagtgat gcagcaataa tagtaataga
     6181 tgcaacagcg cctattcaag ttggtacaga gaaatcatta gaactaactg agagtattcc
     6241 taagataatg tttataaata aaatagataa tgaaaaggca agatacaaag atgcaatagc
     6301 aatgttaaga gaaaaataca ataataagat agttcctatg ataagtccta tatacaaaga
     6361 taaaaacttt gtaaagttac ataatgtttt tgaaaacata gatgatttag aaggtgaatt
     6421 taaggaacaa gctatgagtg ttaaagaagc attaatggaa cttatagcag aaactgatga
     6481 ccaaatatta gataaatatt ttaatggaga agaactaact acagaagaga tacaaaaagg
     6541 tattattata ggtatacaaa gaggtgatat tatacctgta atatgtggtt caactataaa
     6601 taatataggc acaaaggaaa tattagatac aatatcaagt tatttagaac ctatctttac
     6661 agaggaaagc aaaccattta ggggattagt atttaaaact atggttgacc catttgttgg
     6721 aaagatgtca tatataaaaa ttactgaggg tgtactttct aaggataaag atgtttttaa
     6781 tataaataaa aatgtaaaag aaaaaatagc taatatatat acattgagaa atagtgaatt
     6841 agtagaaata gaaaaagcta aagctggtga tatagtagtt ataactaaag ttaattctct
     6901 aaaaacagga gacactatat ctgcagataa agatgcagaa gctttagaaa agatagattt
     6961 tcctaaacca caaatatatt atgcagtaac tcctaagaat aaaggtgatg aagaaaaagt
     7021 agcatctgta ttaaataaac tagtagaaga ggaccctaca ttacattggt atagaaatac
     7081 agagacaaaa caagcacttt taggaggtca aggagaactt catataaaaa caattaaaaa
     7141 taaaatgaaa gataagtttg gtgttgatgt tgaattgaat gaccttaaag ttccttatag
     7201 agaaactata aaaggaacag ctgatgtaca aggaaaacat aaaaagcaat caggtggtca
     7261 tggtcaatat ggagacgtaa aaataagatt tgaaagatgt gaatctgatt ttgaatttac
     7321 agaagaaata tttggtggtt ctgtaccaaa acaatatata ccagcagttg aaaaaggttt
     7381 aaaagactct atgcaaaaag gtatattagc aggatatcct gttacaaaca taaaagctac
     7441 gctttatgat ggttcatacc atgatgtaga ctcttctgaa atggccttta agatggcagc
     7501 aagtgcagcc tttaaaaaag gtatggaaga agcacaccca atactactag aacctataat
     7561 gaaacttaag ataactgttc cagaagaata tatgggagat gtcatgggtg atataaataa
     7621 gagaagagga aaaatatttg gaatggaacc agatgacaaa gggaaacaaa taatatttgc
     7681 agaagctcca caagcagaga catttaaata tgcaatagat ttaagagcaa tgacacaagg
     7741 tagaggatac tttgaaatgg aattagagag atatggagag gttccatcac agtttgcaga
     7801 aaaaataatt gggttagcta ctgcaaagta atgaacaaaa taaatagaaa aataattaat
     7861 atatattatt aaaaatattt ttagggtttt aactaggtat aggtttatgt taggtaaact
     7921 tatacctagt ttttatacat ttttgatgaa taaaaaaata tataatactt tcttaaatat
     7981 tttaataaaa agaaagtatt tttttattat tatttttaca aatgtttagt aataaagatg
     8041 agaaggtata tttaaaatat aaagtattta aagttctgaa atctaatca
//
LOCUS       DAELLV010000166         8001 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.166, whole genome shotgun sequence.
ACCESSION   DAELLV010000166 DAELLV010000000
VERSION     DAELLV010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8001
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.166"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..728
                     /locus_tag="KPD58_002759"
     CDS             <1..728
                     /locus_tag="KPD58_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBG0266495.1"
                     /translation="GIFIPMGIFLPIVCKNLNKKTIIIVIILVSLAFELTQYIFALGS
                     SDIDDVILNSLGGIIGINIYINMNKLFPSDINRFKAIIGTSLVLGVIGLGVISKNYHN
                     LLTFKFRPDRKISKILIEENREIIKDINKDTVDIVGTFESFKKGIITIKAGSNNKVKR
                     PENLIDSDGNIRIYLNENTKLVSYIITKESKMDIVKYEEFDVKNLDLLRRYDTINVWI
                     DKENQPKDKHGIMASKLLIGLYE"
     gene            843..1628
                     /locus_tag="KPD58_002760"
     CDS             843..1628
                     /locus_tag="KPD58_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266496.1"
                     /translation="MEIIKYILYFFIYSFLGWTVESIGCSIASKRIINRGFLNGPICP
                     VYGFGAVIVISLLGRFNNVVIVFLLGMILTTILEYFTGFILETLFHAKWWDYSDRKFN
                     IKGRVCLKNAIYFGVMSVLIIRFIHPFIKYFVSIIPYRILISMAIITTLWTILDLIVT
                     IITLKKLDIKLNLLDDIITDLNDINVKLDKFDRGEIQALFKTINNDELEVREKINKIN
                     SKLDRIESNIILQKRVIKAFPHIKHKKQQEQLEHFKKIINEKR"
     gene            1920..3239
                     /locus_tag="KPD58_002761"
     CDS             1920..3239
                     /locus_tag="KPD58_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG0266497.1"
                     /translation="MSEITSQMISTEEKKYVAKTQKIPYYPVAFKSGDGAMLYDYEGN
                     EYVDFLASAGSANVGHGNKEISQAVKEQMDDITQYTLAYFHSDPPVKLAEKLVEIAPG
                     DNDKKVLYSATGSACIDAAIKLARGYTGRTKIISMCESYHGSTYGAISISALSTNMRR
                     KMGPLLPEVYHFHYPDKNRTAKECLDEIEYAFAHYLPAEEVAAIFIEPIAGDAGIIVP
                     PVEWVQGLSKICKENGILLVSDEIQQGMGRTGKWFGIENFGVEADLIVLGKSVGGGLP
                     LGAVVGRTEIMQSLDAPAHLFTLAGNTTVCVAALKSIEIIEKENLLQKSIEMGDYIKA
                     GFEKLKEKYDIIGEIRGIGLSIGVDIVKGKGSNEKHPDATAKICYRCIQTGLIMIFLG
                     QSTLRVQPPLVITKEQVDKAMNIIDSAIDDYLNGRIGDEVYEVTQGW"
     gene            complement(3314..3742)
                     /locus_tag="KPD58_002762"
     CDS             complement(3314..3742)
                     /locus_tag="KPD58_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266498.1"
                     /translation="MKDNNNTNKTIQFGEKKASLENNIQKNKNIKLDNTHKVIPYPKN
                     RKKDDKKKGNKKKNTNTSKVNKNNYGHSFFNNKYKYKRKPYVIALYTVILILLAILII
                     TKLTDSTNKGNYSRNTVSDTHKNDIRNIVFNESNNFLICG"
     gene            complement(3833..5209)
                     /gene="hydG"
                     /locus_tag="KPD58_002763"
     CDS             complement(3833..5209)
                     /gene="hydG"
                     /locus_tag="KPD58_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HBG0266499.1"
                     /translation="MFINHELINSLLEDAKNSTSDDIEKVLDKADRREKLSYKDIATL
                     LEVEDKKQLDRLFSIAGQIKNEIYGNRVVLFAPLYVSNYCVNECVYCGFSKCNKFKRK
                     KLTMEEIKEEVKILEKMGHKRLALEAGEDPKNCDINYILDCLDAIYSTYNENGNIRRV
                     NVNIAATSVDEYKLLKEKGIGTYILFQETYHKPTFIKMHGQSIKNDYYYHLTAFDRAM
                     EAGVDDVGAGVLFGLSDPKFEVLGLMMHNEHLEEKFGVGFHTISFPRLKKAEGMSLED
                     FPHLVSDDMFKKIVAITRLAVPFTGIIMSTRETAEMRNELLKYGVSQISAGSLTGVGG
                     YKAYEDGDNTEQFEVGDHRSPVEVLKELITDGYIPSYCTACYRKGRTGDRFMSLAKSR
                     QIHNVCTPNALTTLNEFLIDYGDEELKIMGKKLIAEEIGKIEREDIRNIVSNNMTALE
                     RGERDLYL"
     gene            complement(5210..6295)
                     /gene="hydE"
                     /locus_tag="KPD58_002764"
     CDS             complement(5210..6295)
                     /gene="hydE"
                     /locus_tag="KPD58_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HBG0266500.1"
                     /translation="MTKNKIKYFIDKLYSINSLSFDELLYLIENIDNEEYNFEQNLRN
                     YLYKQASDMRNKHYGNRVYLRGLIELTNYCKNNCYYCGIQSSNKNIIRYRLSLDEILE
                     CCDIGYKIGYRTFVLQGGEDAYFTDDKICEIVSSIKNKYEDCAITLSLGEKSYDSYKK
                     YFLCGADRYLLRHETATDSHYKKLHPPNIELRTRKECLKNLKEIGYQVGAGFMVESPF
                     QNNEDLVRDLLYLKELNPHMVGIGPFIPHHDTIFKDYKHGDLEKTLLMLSITRLLLPK
                     VLLPATTALASINPSGRNAGLLAGCNVIMPNLSPQEFRKQYSLYDNKAFTGQEACEYH
                     QSLEENIKSLGLEVDYSRGDNIEWRRL"
     gene            complement(6331..7539)
                     /gene="hydF"
                     /locus_tag="KPD58_002765"
     CDS             complement(6331..7539)
                     /gene="hydF"
                     /locus_tag="KPD58_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HBG0266501.1"
                     /translation="MSLNSTPQSVRVHIGLFGKRNAGKSSIINAITNQSAAIVSDIAG
                     TTTDPVFRPMEILPIGPCVLIDTAGLDDVGELGELRIGKSLDVLEKTDIALLVVDCQI
                     GISQEDLSLIEKFNDKNIPHILILNKIDTIKNQSEILNLTKNKVKCPVVSVSSTDKIG
                     IENLKNEIIKVLPKDSTEFKLVSDLIEPNDLVVLVVPIDKAAPKGRLILPQQQVIRDI
                     LDNGAISIVTKEDSLKETLSNLGKKPKLVITDSQVFPQVDKDTPKDIPLTSFSILFAR
                     QKGDLKELINGAYALENLKDGDKILMAEGCTHHRQTDDIGTVKIPNMIRKKTGKNITF
                     EFSSGVSFTEDINKYALVVHCGACMMNRAGMLSRIEKAKSFNVPIVNYGVLIAYVKGI
                     LERSLELFNY"
     gene            complement(7554..7805)
                     /locus_tag="KPD58_002766"
     CDS             complement(7554..7805)
                     /locus_tag="KPD58_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HBG0266502.1"
                     /translation="MKKVAVISAILEEPKECQFKFNEVVSGFKGIIKGRMGIPCEEEG
                     VSIVCIAVVGEMDTINSLTGKLGNIPHVSVKTSISKKEV"
BASE COUNT         2840 a         1108 c         1171 g         2882 t
ORIGIN      
        1 taggtatatt tattcctatg ggaatttttt tacctatagt atgtaaaaat ctaaataaaa
       61 aaactataat tatagtaatt atacttgtta gcctagcatt tgaattaaca caatatattt
      121 ttgctcttgg aagtagtgat atagatgatg ttattttaaa ttcattaggt ggcataattg
      181 gaattaatat ttacataaac atgaataaat tatttcccag tgatattaac agatttaagg
      241 ctataatagg tactagttta gttttaggag ttataggatt aggagttatt tctaaaaatt
      301 atcataactt actaactttt aaatttagac ctgatagaaa aatatcaaaa atattaattg
      361 aagaaaatag ggaaataatt aaagatataa ataaagatac agtagatata gttgggacat
      421 ttgagagctt taaaaaagga ataataacta ttaaagcagg ttctaacaac aaagtaaaga
      481 gaccagagaa tttaattgat tctgatggaa atataagaat ttatttaaat gaaaatacaa
      541 aattagtatc ttatataatt accaaagaat caaaaatgga tatagttaaa tatgaggaat
      601 ttgatgtgaa gaatttagat ttattaagga gatatgatac tattaatgtt tggattgata
      661 aagaaaatca gccaaaggat aaacatggta ttatggctag taaattatta attggattgt
      721 atgaataaaa tattaaacaa gaataaaaga ataacaaata taaaaaacga aaaaactatt
      781 tctataatta tttttacgtt gtgcataaaa taagaacatt agtgaaggag tagagataaa
      841 ttatggagat aataaaatat attttgtact tttttatata ttcattttta ggatggacag
      901 tagaaagtat aggatgttct atagcaagca aaagaataat caacagaggt tttttaaatg
      961 gtccaatatg ccctgtttat ggttttggag cggtaattgt gatatcacta ttgggaagat
     1021 ttaataatgt agtaatagta tttctattag gaatgatttt aactacgata ctagaatatt
     1081 ttacaggatt tattttagaa accttatttc atgctaaatg gtgggattat tctgacagaa
     1141 aattcaatat aaagggtaga gtatgtttaa aaaatgcaat atactttggt gtaatgtctg
     1201 ttttaataat aagatttata catccattta taaaatattt tgtaagtata attccatata
     1261 gaatattaat ttcaatggct ataattacaa cactttggac tatacttgat ttgattgtta
     1321 ctattataac acttaaaaaa ttagatataa agttaaactt attagacgat ataattacag
     1381 accttaatga tataaatgtg aaattggata aatttgatag aggagaaata caagctttat
     1441 ttaaaacaat aaataacgac gaattagaag ttagagaaaa aataaacaaa ataaatagta
     1501 agttggaccg tatagagtct aatattattt tacaaaaaag agttataaaa gcttttccac
     1561 atataaaaca taaaaaacaa caagagcaac tagaacattt taaaaaaatt attaatgaga
     1621 aaaggtaatt atacaattta atgaagacga atagaataat ggtatttata agatgatttt
     1681 cagaggactc tataaaaata gaatagagtc ctttattttt tgaaaaataa tgtttatttg
     1741 ataaaaataa aatatattgt ttctgaataa caaatatttt gaaaattata aaaacatata
     1801 ttgacaattt gtgtcaaaaa tactaacatt aagtatataa agaattttat atgagaatat
     1861 ctttataaat cgcaataatt ttaaaacata aatttattta aagaggggat gatttgccaa
     1921 tgagcgagat aactagtcaa atgataagta cagaagaaaa aaaatatgtt gctaaaactc
     1981 aaaagatacc ttattatcca gtagcattta aatcaggtga tggtgcaatg ctatatgatt
     2041 atgaaggtaa tgaatatgta gattttttag caagtgcagg ctctgcaaat gtaggtcatg
     2101 gtaataagga aatctcacaa gcagttaaag aacaaatgga tgatataaca caatatacac
     2161 ttgcatattt ccatagtgat cctcctgtaa aattagctga gaagcttgtg gaaatagccc
     2221 ctggagataa tgataaaaaa gtactataca gtgcaactgg ttctgcttgt atagatgctg
     2281 ctataaaatt agcaagaggg tatactggaa gaacaaagat aatatctatg tgtgaatctt
     2341 atcatggaag tacttatgga gctatatcta tatctgcttt aagtacaaat atgagaagaa
     2401 aaatgggtcc attgttacca gaagtttatc acttccatta tcctgataaa aatagaacag
     2461 ctaaagaatg tttagatgag atagaatatg cttttgcaca ctatctacca gcagaagagg
     2521 tagctgcaat atttatagag ccaattgcag gagatgcagg aataatagtg cctccagtag
     2581 agtgggtaca aggtttaagt aaaatatgta aagaaaatgg aatattactt gtaagtgatg
     2641 aaatacaaca aggtatggga agaacaggta agtggtttgg tatagaaaat tttggtgttg
     2701 aagcagacct tatagttctt ggaaaatctg ttggaggtgg attacctcta ggagcagttg
     2761 ttggaagaac agaaataatg caaagtcttg atgcaccagc acatttattt actttagctg
     2821 gaaacacaac agtatgtgtg gctgctttaa aatctataga gataattgaa aaagaaaact
     2881 tacttcaaaa gagtatagaa atgggagatt atattaaagc aggatttgaa aaactaaaag
     2941 aaaaatacga tataattgga gaaataagag gcataggatt atctatagga gttgatatag
     3001 ttaaaggaaa aggttctaat gaaaaacatc cagatgctac agctaagata tgttatagat
     3061 gtatacaaac tggattaata atgatattct taggacaatc tactttaaga gtgcaaccac
     3121 ctctagtaat aactaaggag caagtagata aagcaatgaa catcatagat tctgcaatag
     3181 atgattattt aaatggtaga attggtgatg aagtatatga agtaactcaa ggttggtaaa
     3241 ataaatatta aatgaaaata ggagttatct caattttaga aaattatttc tgaaatgaga
     3301 taactctttt aagttaccca caaataagaa aattgtttga ttcattaaag actatatttc
     3361 ggatgtcgtt tttgtgagta tcagagacag tattacgaga ataattacct ttattcgtac
     3421 tatctgttaa tttagttata attaagatag ctaaaagaat aagaattact gtgtataaag
     3481 caataacata tggttttctt ttatatttgt atttgttatt gaaaaaagaa tggccataat
     3541 tatttttatt tactttagat gtatttgtat tctttttctt gttacctttc tttttatcat
     3601 ccttttttct atttttaggg taaggaatga ctttgtgtgt attatcaagt ttgatatttt
     3661 tatttttttg aatattgttt tccaggctag cctttttttc tccaaattga atagttttat
     3721 tggtattgtt gttatctttc aatgttaatc tccctccctt aaactttccc tttagataat
     3781 attactacta acctatggaa tatacaataa ataatatata agtaataaaa ttttataggt
     3841 ataaatctct ttctccacgc tctaaggcag tcatattgtt agatactatg tttcttatat
     3901 cttctctttc tatcttacct atttcttctg cgataagttt tttacccata attttaagtt
     3961 cttcatctcc ataatcgatt aaaaattcat ttaatgtagt aagagcattt ggtgtacata
     4021 cattatggat ttgtcgactt ttagctaagc tcatgaatct atcgccagtt ctacctttac
     4081 gataacaagc tgtacaataa ctaggtatat atccatcagt aatcagttct ttaagcacct
     4141 caacagggct tctatggtct ccaacttcga attgttctgt attgtcacca tcttcatatg
     4201 ctttatatcc tccaacacct gttagagaac ctgcactaat ttgtgataca ccatatttta
     4261 gtaactcatt tctcatttca gcagtttctc tagtggacat tatgattcca gtaaaaggaa
     4321 cagctaatct agttatagct actatttttt tgaacatatc gtcagaaact aagtgaggaa
     4381 aatcttctaa gctcatacct tcagcttttt tcaatctagg gaaagatata gtatggaaac
     4441 ctacaccaaa tttttcctct aagtgttcgt tgtgcatcat aagtcctaga acttcaaatt
     4501 tagggtcaga taaaccaaat aaaactcctg caccaacatc atcaacacca gcttccatag
     4561 ctctatcaaa agctgttaaa tggtaataat agtcattttt tatagattga ccatgcattt
     4621 ttataaatgt tggtttgtga taagtttctt ggaataatat ataagttcct attccttttt
     4681 cttttagtaa tttatactca tctacacttg tagctgctat gttaacgttt acccttctta
     4741 tatttccgtt ttcattatat gttgaataaa ttgcatctaa gcaatctaag atgtaattta
     4801 tatcacaatt tttagggtct tcaccagctt ctagagcaag tcttttatga cccatttttt
     4861 ctaatatttt aacctcttct tttatctctt ccatggtaag tttttttctt ttaaatttgt
     4921 tgcatttact aaaaccgcaa taaacacatt catttacaca gtagtttgat acataaagag
     4981 gggcaaataa aaccactcta ttaccatata tttcattttt tatttgccca gctatactaa
     5041 ataatctatc tagttgtttt ttatcttcca cttcaagaag tgtagctata tctttataac
     5101 taagtttttc ccttctatca gccttatcaa gtaccttttc aatatcatca gaagttgaat
     5161 tttttgcatc ttctaataaa gaattaataa gttcatggtt tatgaacatt tacaaacgcc
     5221 tccattcaat attatcaccg cgagaatagt caacttcaag tcctaatgac ttgatgtttt
     5281 cttctaaact ttggtgatat tcacaagctt cttgaccagt aaaggctttg ttatcgtaaa
     5341 gagaatattg ttttctaaat tcttgaggag aaaggtttgg cataattaca ttgcaaccag
     5401 ctaaaagacc tgcatttcta ccactagggt ttatagatgc caaggctgtt gtagcgggta
     5461 ataaaacttt tggaagtaga agtcttgtaa ttgaaagcat aagtaatgtc ttctctaaat
     5521 ctccatgttt ataatcctta aaaattgtat catggtgtgg aatgaaaggt cctattccaa
     5581 ccatatgagg atttaactct tttaggtata ataaatcacg aacaaggtct tcgttgttct
     5641 gaaatggtga ttcaaccata aatccagcac ctacttgata accaatttct ttaaggtttt
     5701 ttaggcattc ttttctagtt ctaagctcaa tgtttggcgg atgtaatttt ttataatgac
     5761 tgtctgtagc agtttcatgt cttaaaagat atctatctgc accacataaa aagtattttt
     5821 tatacgaatc ataactcttt tctcctagtg atagagttat ggcacaatcc tcatatttat
     5881 tttttatact tgaaacaatt tcacatattt tatcatcagt aaaatatgca tcttcaccac
     5941 cttgcaaaac gaatgttctg tatcctattt tataacctat atcacaacat tcaagtattt
     6001 catcaagtga aagcctgtat cttatgatat ttttattaga agattgtatt ccacaatagt
     6061 agcaattatt tttacaatag tttgtaagct ctataagtcc tctcaagtag actctatttc
     6121 cataatgttt atttctcatg tcgctagctt gtttataaag atagtttctt aaattttgtt
     6181 cgaagttata ttcctcattg tcgatatttt ctattaaata tagtagttca tcaaaactta
     6241 atgaattaat gctgtaaagt ttatctataa agtatttaat cttattttta gtcatattca
     6301 aatatgtaat tattaagtat agttaaattt ttaataattg aaaagttcaa gtgatctttc
     6361 caaaatacct tttacatatg ctattaagac accatagttt acaataggta cattgaaaga
     6421 ttttgccttt tctattctag ataacattcc agctctattc atcatacaag ctccacagtg
     6481 aactacaagg gcatatttat ttatatcttc agtgaaggat actccagaac taaattcaaa
     6541 agtaatgttt ttgccagttt tctttctaat catattagga atttttactg ttccaatatc
     6601 atcagtttgt ctatgatggg tacatccttc ggccatcaag attttatcac catcttttaa
     6661 attttctaaa gcataagctc cattaatcaa ttctttaagg tctccctttt gtctagcaaa
     6721 caatatagaa aaagatgtaa gaggaatatc ttttggtgta tctttatcta cttgtggaaa
     6781 tacttgagag tcagtaataa caagttttgg ttttttgcct aaattagata gagtttcttt
     6841 taaactatct tcttttgtaa ctatagaaat tgcaccatta tccaaaatat ctcttataac
     6901 ttgctgttga ggaagtataa gtctaccttt tggagctgct ttatctattg gaacaactaa
     6961 aacaactaaa tcatttggtt caattaaatc tgataccaat ttgaactcag tgctatcctt
     7021 aggcaaaact tttattattt cattttttaa attttcaatt ccaattttat ctgttgatga
     7081 gacagaaaca actggacatt taactttatt tttagttaag tttaaaattt ctgattgatt
     7141 cttaatagta tcaattttgt taagaattag aatatgaggg atattcttat cattaaattt
     7201 ttcaattagt gacaaatctt cttgtgatat accaatttgg caatccacta ctagtaaagc
     7261 tatatctgtc ttttctaaaa catctagtga tttacctatt ctaagttcac caagttcacc
     7321 aacatcatca agaccagcag tatctataag aacacaaggt cctataggaa ggatttccat
     7381 tggtctaaat actgggtctg tagtagttcc agcaatatct gagactattg cagcactttg
     7441 atttgttata gcatttatta tgctagattt acctgcattt cttttaccaa agagacctat
     7501 atgtactctt accgattgtg gagtagaatt tagactcatt tgtactcacc accttaaact
     7561 tctttttttg atatactagt ttttacagat acatgaggta tgttgcctaa tttaccagtc
     7621 aaactattaa tagtatccat ttcgccaaca actgctatac atacgattga gacaccttct
     7681 tcttcgcaag gaatgcccat tcttccttta ataatacctt taaaacctga tacaacctca
     7741 ttgaatttaa attgacattc ttttggctct tccaaaatag cacttataac tgcaaccttt
     7801 ttcataaata tcctcctaac tcagaaaata tgtcttaaac aaatccaaaa tcaaaaaact
     7861 tttctttgta aagaaaagtt tctacaaaag aaaagttatt aattacacaa gtataatgtg
     7921 ctatattaaa ctatttcctt acagattaga taactctttt ctggtagggt tagcttgtaa
     7981 aaatttaatt taacaacaag t
//
LOCUS       DAELLV010000167         7986 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.167, whole genome shotgun sequence.
ACCESSION   DAELLV010000167 DAELLV010000000
VERSION     DAELLV010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7986
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.167"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(65..1348)
                     /locus_tag="KPD58_002767"
     CDS             complement(65..1348)
                     /locus_tag="KPD58_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011572945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBG0266503.1"
                     /translation="MTKEEYYEERQKLIDQAQKLLDDEKMEEAEEVTNKIKNLDSSFE
                     TEVKARANLRALEDDNDEIHPIIADMMFNNGSISLSNGETSYNVTSQPIEKNNLYSYT
                     AREGNKIRALGKKDSFRSSLNINNSQNLSLGKYIKGMHIGDWKDANVEMEAYKALNTS
                     TGSTLIPRELSAEIIDLARNKMALSNINVIPMETNNLTLAKVKSDPKFSFKKELEVAT
                     QSDMEFEGIELRTKTVYGYMKISLELLESAGNIDAIISNAIAESIASAIDKAGLYGVG
                     ENEPKGILTYTGINKVNESLISTSKYNGLVKGIGLIKKANGEATDILYNSNTETDLNL
                     LVDTTGQPLNEPKVVEQIEKTTSNQIKDNQAIVFDRNSILMGLQNNIRIETSREMGFT
                     DGSVYFRVYGFVDFVVTNEKNITLIEYSKTPQDAE"
     gene            complement(1901..2356)
                     /locus_tag="KPD58_002768"
     CDS             complement(1901..2356)
                     /locus_tag="KPD58_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011572947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266504.1"
                     /translation="MLNSLLNDDNIGTYTYNYINSMFGVGTSYTKEHLEALSVDLQTF
                     LATKKKLSIKKISLILQSIFVIEVKQHRGVTGLPYKYYKNILPNPVTDKKVIRVYTIK
                     KHIDVEDIKKELGLMDSDLSIEHLVEARINKLLNTIDEADKEVLLEGLI"
     gene            complement(2493..5102)
                     /locus_tag="KPD58_002769"
     CDS             complement(2493..5102)
                     /locus_tag="KPD58_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011572948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG0266505.1"
                     /translation="MKIDIKQLIDIIKNANIDELYLRDIGVDTTQKGNVPCPIHGGKD
                     KNFQFDLNKRIYTCYSHNCVVGADIIELCRTYEHFEKPIEAILFLANKYNVPLPFTKN
                     KTNTKKTNNTIKNIYIKKKDINSFIEEKKQEAFKNNDIELAFEFECMTDKDKRKYYLD
                     NNIKIKSMLEKQNYSSYRADSIIDVDKYISENLKGIRQAIECTTEGENVLMVAPTGSG
                     KTYSIINTLKELDIKALFIFPNSANVQQAIVEYNIAGAYDKIPTKHALKDNNLVAMTW
                     DKTEQLLNEDLSEYIIVIDEIHQTYTDAFRSKAIKSLNNITNKCRGRLDITATPSKLE
                     FEIYNKVIEYKQNIQTEYKVKLYNKINIGKVIEILNNSNNAMLLKDSISTLNYISKKV
                     NKKSGVVISDTKDTSKLYDRIVSYSDMEGYEVLLNTSVIVAGVNINNPNITDIVIIGE
                     KDIGKIKQYVARARGAKSINVHIFNSYKTTNEDSNVYSVEWCINENIKDVESLTNIYN
                     KASKRDLPFKAIGIDISPINIKNIDSNIYYDKKDMCYKTDKLYIKSNAYNNYYQTRAI
                     DSFKVLLEEYFNKIEIIETEKETKKIEEEIKEHEEAIKEFKKSAIEQLEGHKKYLVGY
                     SEIKKSMTSSNLLKYHHDMRIDNNICLKHYMEHDLYSLIINNNCRKTIDLFSGFVLDN
                     SYSIDLAWKLATMGNKGRWSIFDKINKLIYRELKEQYPNFVNDELIGTVVYDYIIKRF
                     GIGTNYTKIHLQELSKDLQILTGAKKMKTTETVGTILNNIFVISSKNYRNVAPLEMCF
                     YKYIYPMGATDNKKVTRINKIERYITLNDIKKELDLKDSDRSLEHLVRNRKDKLLQTI
                     DSDDKEILLNGLA"
     gene            complement(5377..5679)
                     /locus_tag="KPD58_002770"
     CDS             complement(5377..5679)
                     /locus_tag="KPD58_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266506.1"
                     /translation="MIKDDLLRAGFDTYGVNLEDKLLENDEIYLNDKKESERIMNRLL
                     EILEEKEHKKMLIDFEEKVFGNSVIEKEYAYRMGVLDGLDLAEFINLTKKLCLKRN"
     gene            5823..5972
                     /locus_tag="KPD58_002771"
     CDS             5823..5972
                     /locus_tag="KPD58_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HBG0266507.1"
                     /translation="MLKNRVRFSSSTKPDLFEKLKQLSEQTRIPLTKLIDEALEDLIK
                     KYKLD"
     gene            6045..6590
                     /locus_tag="KPD58_002772"
     CDS             6045..6590
                     /locus_tag="KPD58_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011572951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266508.1"
                     /translation="MYRYTFDNLPIEEQIKYINNKLLEGNTLTNTCKDIGIGRTTVRN
                     RFKQHGYEFNQAKKLYISIIEVVELESSSKVVEGDTSNDNTLVDATNIDFNNILSKFN
                     DMNNKLNEVYSWYESQSSNKVVGADKLKINDFKGNTVTRSYKVYEDIQKEFVAFCEAN
                     KKYRVQDIISQALKDFMDKYK"
     gene            complement(6773..6973)
                     /locus_tag="KPD58_002773"
     CDS             complement(6773..6973)
                     /locus_tag="KPD58_002773"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266509.1"
                     /translation="MNIQVYESECGNYIFKKSDESFLIDIFDKNGTHMGAFGMPSIKT
                     NTICGEKGKFISACDAWLSNLK"
     gene            complement(7106..7591)
                     /locus_tag="KPD58_002774"
     CDS             complement(7106..7591)
                     /locus_tag="KPD58_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG0266510.1"
                     /translation="MIELKKEYIDKTIKLLDEVRELKIRKEVLNDEIEFLKKNEKLSS
                     INFNELGFPIHSGNYGIDDMIINLQEKIYIKETEIEIIDSRIEMINIYTKRLSEEEQE
                     IISLRHFDKKVNSYEKISELLMISKTAVQKKYTYALKKIALMKYGEEAKKNMANSISP
                     P"
BASE COUNT         2448 a         1304 c          879 g         3355 t
ORIGIN      
        1 aaaaaaaata ctactctatt tattatcttt aaagccctct aactaaatta gaaggctttt
       61 ttatttattc agcatcttgt ggagtttttg aatactctat tagtgttata tttttctcat
      121 tcgtcactac aaagtcaaca aaaccataaa ctctaaaata tactgaacca tctgtaaagc
      181 ccatttccct tgatgtttct attcttatat tattttgaag tcccattaat atagaatttc
      241 tatcaaatac tatagcttga ttatctttta tttgatttga agttgtcttt tcaatctgtt
      301 caactacctt aggttcattt agtggttgtc ctgttgtatc aactaataag tttaaatctg
      361 tttcagtatt agaattatat agtatatccg tagcttctcc attggctttt tttataagac
      421 caattccttt tactaaccca ttatactttg atgtagatat taaactttca tttactttat
      481 ttattcctgt atatgtcaat atcccttttg gttcattttc tccgacccca taaagtccag
      541 ctttatctat tgcacttgca atagattctg ctatagcatt tgatataatt gcatctatat
      601 taccagcact ttctaatagt tcaagtgaga ttttcatata tccatatact gttttggttc
      661 ttagctctat cccttcaaat tccatatctg attgagttgc aacttcaagc tcttttttaa
      721 agctaaattt ggggtcactt ttaactttag ctaaggttaa attattcgtt tccattggta
      781 taacattaat gtttgataaa gccattttat tcctagctaa gtcaattatt tcagctgaca
      841 actcacgtgg tataagtgtt gaacctgtac tagtattcaa agccttataa gcttccattt
      901 caacattagc gtccttccag tcacctatgt gcataccttt tatatatttt cctaaactta
      961 aattttgaga attatttata tttaaagaac ttctaaagct atctttttta cctaatgctc
     1021 taattttatt accttcccta gctgtataag aatacagatt atttttttca attggttgtg
     1081 atgtaacatt atatgacgtt tctccatttg ataaacttat acttccgtta ttaaacatca
     1141 tatctgctat tattgggtga atttcatcat tatcatcctc taatgctctt agattcgctc
     1201 gagcttttac ttccgtttca aatgaactat ctaaattttt tattttatta gtaacttctt
     1261 cagcttcttc cattttttca tcatcaagca acttttgtgc ttggtctatt aatttttgtc
     1321 tttcttcgta atactcttct ttagtcattt atatttcctc ccaatttaaa taagcccaat
     1381 ttgaattgga ctatttttta ttatttctct ttttaagata taaataaata attttaaata
     1441 cattcttctt atccactatc ttttttaatt cctagttgca aataaagtca cctccaaaac
     1501 ttttcaataa aaaaagacct ctaaataaaa cttagaaatc tttttaattc ttatatgttg
     1561 caattatata ttatttattt agaaagtaat cttcataact ttctacaata taattatagc
     1621 acacgataat atttttattt gtatcttttt tttccccaat ttagttcacc tttaaaccat
     1681 aaaaaattca ctaaaaaatc actagaaata cttttttcta tgatacaatt ctaacatgct
     1741 ttctttagaa acgaagtata aaaatagtct tttttctgta aaaataatgt ttattctaaa
     1801 gcttcatcac catatatctt caaaataatt ttatgctcca aaatcaagcc cttaaaagtt
     1861 ttttattgtt acataactct tgtaaatata tttttttatg ttaaattaat ccttccaata
     1921 aaacttcctt gtctgcttca tcaatcgtat tcaatagctt atttattcta gcttcaacta
     1981 agtgctctat gcttaaatca ctatccatta atcctagttc ttttttaata tcctctacat
     2041 ctatatgttt cttaatagta taaactctaa tcactttttt atctgtcaca gggtttggaa
     2101 gtatattttt atagtattta tagggcaacc ctgtgacacc cctgtgttgt ttgacttcaa
     2161 taacaaaaat tgattgtaat attaagctta ttttcttaat agacagcttt ttcttagtag
     2221 ctagaaacgt ctgtaaatca acgcttaggg cttctaagtg ttcttttgtg tagcttgtgc
     2281 caactccaaa cattgaatta atatagttat aagtataagt cccaatatta tcatcattta
     2341 gcaaagaatt taacaaaata tcagcttttt taatgaaatt ttagcgaatt tattcctatt
     2401 tcaattaata ttagtttgat ttctaacctt ataaatcttt ttaaattaat ctcttttaag
     2461 cattcctttt caatgtcaaa ttgcccttta ttttatgcta atccatttaa taatatctct
     2521 ttgtcatcac tgtctatagt ttgcaataac ttatctttac gattacgaac taaatgctct
     2581 aagcttctat cactatcctt taaatcaagt tcttttttga tgtcatttag agtaatatac
     2641 ctctctattt tgttaattct agttactttt ttattatctg tagcacccat agggtatata
     2701 tatttataga aacacatttc taagggtgct acatttctat aatttttact gctaattaca
     2761 aaaatattgt ttagtatagt acctacagtt tcagtcgttt tcattttttt tgctcctgta
     2821 agaatctgca aatctttgct taattcttgt aaatgtattt ttgtatagtt tgtacctatt
     2881 ccaaatcgtt taattatata atcataaact actgttccta taagttcatc atttacgaag
     2941 ttaggatact gctccttaag ctctctatag ataagtttat ttatcttatc aaatatagac
     3001 catctgccct tgtttcccat tgttgcaagt ttccacgcaa ggtcaatact ataactatta
     3061 tctaatacaa aaccactgaa caggtctatt gttttcctgc aattattatt aattattaga
     3121 ctatataaat catgttccat gtagtgcttt agacatatgt tgttgtctat cctcatgtca
     3181 tgatgatatt ttaataagtt gctgctggtc attgacttct taatttcact atagccaact
     3241 agatactttt tatgtccttc tagttgttct atagctgatt tcttaaattc cttaatagct
     3301 tcctcatgct cttttatttc ttcctctatt tttttagttt ctttttcagt ttctattatc
     3361 tcaattttat taaaatattc ctctaatagc actttgaagc tatctattgc tcttgtttga
     3421 tagtagttat tgtaagcatt actttttata tataacttgt ccgttttata gcacatatcc
     3481 tttttatcat agtatatatt actatcaatg ttttttatgt ttatagggct tatatcaatt
     3541 cctattgctt taaatggtaa gtctctctta gaagctttat tgtatatatt tgttaagctc
     3601 tcaacgtctt ttatgttctc atttatgcac cattcaaccg aatatacatt gctatcctca
     3661 tttgtcgttt tgtaactatt aaaaatatgt acatttatac tttttgcccc tctagctctt
     3721 gcaacatact gttttatttt tcctatatct ttttccccta ttatgactat atctgttatg
     3781 ttcggattat ttatatttac acctgcaact atcacagaag tgttcaaaag cacctcatag
     3841 ccttccatgt cagagtagct gacaattcta tcatatagtt tgctagtgtc tttagtatct
     3901 gaaataacaa ccccactctt tttattaact ttctttgata tatagttaag agttgatata
     3961 ctatctttta aaagcattgc attatttgaa ttatttagta tctctattac tttcccaata
     4021 ttgattttat tgtataactt aactttgtat tccgtttgta tattttgttt atattcaata
     4081 actttgttat atatctcaaa ttctagttta gagggtgttg cagttatatc tagtctacct
     4141 ctgcacttat tagttatatt atttaagctc tttattgctt tgcttctgaa cgcatctgtg
     4201 taggtctgat gtatttcatc aataactatt atatattcgc ttaaatcctc atttaaaagc
     4261 tgttcagttt tatcccatgt catggctact aaattgttgt ccttcaaagc gtgtttagta
     4321 ggtatcttat catatgcacc agctatatta tattcgacta ttgcttgttg cacattcgca
     4381 gagttgggga atataaagag tgcttttata tccagctctt ttaatgtatt tataattgag
     4441 tatgtttttc cacttcctgt aggtgctacc attaatacat tttcaccctc tgtagtacac
     4501 tcaatagcct gtctaatgcc ttttaaattt tcacttatgt atttatctac atcaattatt
     4561 gaatcggctc tatagcttga atagttttgt ttctctagca tagattttat tttaatattg
     4621 ttatctaagt aatactttct tttgtcctta tcagtcatac actcaaattc aaaagctaac
     4681 tcaatatcat tgtttttaaa agcttcttgt ttcttttctt ctatgaagct atttatatct
     4741 tttttcttta tgtaaatgtt tttaatagta ttatttgttt ttttagtgtt ggttttattt
     4801 ttagtaaatg gcaatggaac attatacttg ttggctaaga atagtatagc ttcaataggc
     4861 ttttcaaagt gttcatatgt acgacataac tcaattatgt ctgcaccaac aacacaatta
     4921 tgactatagc atgtatatat tcttttattt aaatcgaatt gaaagttttt atccttacca
     4981 ccatgaattg ggcaagggac atttcctttt tgagttgtat ctactcctat atctcttaaa
     5041 tatagttcgt ctatattagc attttttatt atatcaatta attgttttat atctattttc
     5101 atttgtgaat ttcccccttt tttttattag gggctaatca agttttatca agctatgtat
     5161 ttacaatctg tcgtaaatct cgtataatta acatatgaat attttatcta tgttaatttt
     5221 atttttcaat atgattaacc cttaaatgga aagaatgagt ggttgccgtc aaacaaatac
     5281 tcgttctttt tcgttttttt gttattagta ttatatcatt aataaaatta taattcattg
     5341 tttgaagtta ttttttgacg gttttaatat atatttttaa tttcttttca agcataattt
     5401 tttagttaag tttataaatt cagctaggtc aagcccatct aaaaccccca ttcggtatgc
     5461 gtattctttt tcaattacac tgttaccaaa tactttttcc tcaaaatcaa ttagcatttt
     5521 tttatgttct ttttcttcca atatttctaa caatctgttc attattcttt cagattcttt
     5581 tttgtcattt aaatatattt cgtcattttc tagtagttta tcttctaaat ttactccgta
     5641 tgtgtcaaat ccagccctta ataaatcatc ttttatcact tttaacgctc cttttttata
     5701 atttatacaa ttaaaataac ataaggtagc ataactttca agctttaaat ggtaattgat
     5761 gttacttgct ttaatgtcat gacatatgta taatatattt aaatacgata caggaggaaa
     5821 acatgttaaa aaatagagta cgcttttcat cttcaacaaa acctgattta tttgaaaaac
     5881 taaaacaatt atctgaacaa acaagaatac ctctaactaa attaatagac gaagccttag
     5941 aggatttaat taaaaaatac aaattagatt aatattttta aaggttctta tttataacta
     6001 ctagaaagct actttagtag ttattttatt ttggaggttt aaacatgtat agatatacat
     6061 ttgataattt acctattgag gaacagataa aatatattaa taacaagctc ttagagggca
     6121 atacattaac taacacatgt aaagatattg gtattggtcg tacaactgta agaaatagat
     6181 ttaaacaaca tgggtatgaa tttaatcaag ctaagaagct atacatatct attattgagg
     6241 ttgtcgaatt agaaagtagt tcaaaagtag ttgagggcga cactagcaat gacaatactt
     6301 tagttgatgc aactaacatt gattttaata atattttatc taaattcaat gatatgaata
     6361 ataaactaaa tgaagtatac agctggtacg aatcacaaag tagtaataaa gtagttgggg
     6421 ctgataaatt aaaaataaat gattttaagg gtaatacagt cactagaagc tacaaggtat
     6481 atgaagatat tcaaaaagag tttgtagcct tctgtgaagc taataagaag tacagggtac
     6541 aagatattat tagccaagct cttaaagatt ttatggataa atataagtaa caatataact
     6601 actcctaaaa ttgacgttta gggaatagtt aaacatattt cagcattttc aacctttccc
     6661 taaacttcta ttttgaaact ttaaaatata aagttttagt gttttttatt ttattaacta
     6721 ttaaacacct aacaaactta aaaactttga ttatttctag tttttattat ttttacttta
     6781 aatttgataa ccaagcatca catgcactta tgaattttcc cttttctcca caaattgtat
     6841 ttgtcttaat actgggcatc ccaaacgctc ccatatgtgt tccattttta tcaaatatat
     6901 ctattaaaaa actttcatca gattttttaa atatataatt tccacattca ctttcataaa
     6961 cttgaatatt cattttaata actccttttc ctttttaatg ttcgtaaatc attttaaatc
     7021 accccccctc ccttttcatt ttttcattcg gaggaaaatt gatgtggggg catcggtatt
     7081 tgaaatcagc cccccagggt cgtttttagg ggggggatat actatttgcc atattttttt
     7141 tagcttcttc tccatatttc atcaatgcaa ttttcttcaa agcatatgta tatttcttct
     7201 gaacggctgt tttagaaatc attaataatt cagaaatttt ttcataggaa tttacttttt
     7261 tatcaaaatg cctaagactt ataatctctt gttcttcttc acttaatctc tttgtgtaaa
     7321 tattaatcat ttctattcta ctatctataa tttcaatttc tgtctctttt atataaattt
     7381 tctcctgtaa atttataatc atgtcatcta tgccataatt cccagagtgt attggaaacc
     7441 caagttcatt aaaattaata ctactaagct tttcattctt tttaagaaat tcaatctcat
     7501 catttaaaac ttcttttctt attttaagtt ctctgacttc atctaaaagt tttatagttt
     7561 tgtcaatata ctcttttttt aattcaatca attctttcat aaaacacctc tttttgatac
     7621 gaatatcata atacatctaa atacaattcg catttcctct taaaaatccg ctattttctt
     7681 agcaaatcaa ggatttgaac ttaagtaata taatagttat aaatcattgc taaatctaac
     7741 ttgaaataat ttaatttaat tttcttatat gtaaaatcct actacaccaa attattaagg
     7801 atttaccttc aaattaaacg aactttaaat cctataacca gttaaataat tcgtatttat
     7861 tgatttgaac gtcttatttt cttagggaac cagatattca acttaaattt ttcctacatt
     7921 atatgcactg aatttgaccc ctcgaatgtt actacaccaa atttttagac attcaaataa
     7981 ataaat
//
LOCUS       DAELLV010000168         7942 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.168, whole genome shotgun sequence.
ACCESSION   DAELLV010000168 DAELLV010000000
VERSION     DAELLV010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7942
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.168"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(67..618)
                     /locus_tag="KPD58_002775"
     CDS             complement(67..618)
                     /locus_tag="KPD58_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan transporter"
                     /protein_id="HBG0266511.1"
                     /translation="MKTNTKKLTLNAILLAMGLLIHQITPAIGLPMKPDVPLAMLFVI
                     LVLNRDDYKTCLIAGIVTGIFTALTSSFPGGQIPNVIDKTLTANIVFLLMNISYNMPF
                     IKKLAKKTQDTVVVAIIMPIGTLVSGTIFLLAAQAIVGLPGASFTALFLAVVLPAVLI
                     NLVAGIFLFKIVSLSIRRVSYQA"
     misc_binding    complement(769..1032)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1468..1866)
                     /locus_tag="KPD58_002776"
     CDS             complement(1468..1866)
                     /locus_tag="KPD58_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266512.1"
                     /translation="MNKKKKLIGIAISFLLIISIINPLQVEAEAIIESSVYISSIKEN
                     YTYDNTKFKTIKSKSRHKSRKYNKSKVKLKKRSIRTKKYGYQTINKYKRQNTRRHNIL
                     TKLILSIIVIFLVIVLVVLIFIKRKKKKFY"
     gene            complement(2221..2706)
                     /locus_tag="KPD58_002777"
     CDS             complement(2221..2706)
                     /locus_tag="KPD58_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBG0266513.1"
                     /translation="MISELMLKNFRKSSINFKTKIKLNLREKPDINSLKLKSIPEGKI
                     VKLKCVDGIWAEVESNYDKGWLLYKYLERLSNAKNNSNDTIKNRKKNYIGNIRTNGLS
                     LELRNDRTLESKVITTIPDGFKVEVCYSVGKWARVNINKNEKRYSGYVYNQYIEEHLS
                     L"
     regulatory      complement(2789..2882)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(3238..3621)
                     /locus_tag="KPD58_002778"
                     /pseudo
     CDS             complement(3238..3621)
                     /locus_tag="KPD58_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     regulatory      3862..3959
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            4014..4190
                     /locus_tag="KPD58_002779"
     CDS             4014..4190
                     /locus_tag="KPD58_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266514.1"
                     /translation="MFGNKMDKCTHVLTAYISSSYDYCNFLDTQLDDFILEYGESVVE
                     SCLHQVMVLVSKYN"
     gene            4416..4616
                     /locus_tag="KPD58_002780"
     CDS             4416..4616
                     /locus_tag="KPD58_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBG0266515.1"
                     /translation="MNNGIVKWFNNEKGFGFISMEGRDDVFAHFSAIQTSGFKSLEEG
                     QQVSFDIVKGARGPQAENITIL"
     repeat_region   4981..5148
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=4981..5008
                     /rpt_unit_seq="tttacataccacatagttgatataaaac"
     gene            5366..5548
                     /locus_tag="KPD58_002781"
     CDS             5366..5548
                     /locus_tag="KPD58_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBG0266516.1"
                     /translation="MFKFYNNATLMFLSGTNIKTISERLGHADIKITMNSYSQFLEEM
                     DREAFENLSKILFIIF"
     gene            5619..6464
                     /locus_tag="KPD58_002782"
     CDS             5619..6464
                     /locus_tag="KPD58_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266517.1"
                     /translation="MNIIKSITLLLTLLIVILMTGCSPRESASEVAKKYIDKMNSGDY
                     ETAYSLLSKDSKKKISIENLEEFQNILVSTKRIISYKIGKEKIIKKYKHDGKEYKNVV
                     KLEETFNIKNLLYKKDDSLKVNRYFVVENNKYKLLLYESFKKEFGINSMDLAQLKLND
                     FGLKYFKEVDLILKKAIAINPTDSKLYYAQACNYMNFDGFPGYSDTTYNALDSINKSL
                     KYLDKNDSDYKKNASNIYNLKGVILMFHKRFDEAQQYLNKSLYFNKDNEDPKYNLNTI
                     KKHLN"
     gene            complement(6688..6852)
                     /locus_tag="KPD58_002783"
     CDS             complement(6688..6852)
                     /locus_tag="KPD58_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266518.1"
                     /translation="MFGKPKESVRFLDFNDVAEKFGEKKAVAIFDAIAGKKSKEHIIK
                     DIEKLVKNEV"
     gene            complement(7394..7726)
                     /locus_tag="KPD58_002784"
     CDS             complement(7394..7726)
                     /locus_tag="KPD58_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBG0266519.1"
                     /translation="MNTREMKEYRVFYNKKLSETDEFFRVFGSLDDKTYSEGAISKKN
                     KELMGLAISILSRCDECICYHIEGCINSGASKDEIIEAIKIGVIGGGSITYPNARFAI
                     KILEEFNI"
BASE COUNT         2854 a         1088 c          792 g         3208 t
ORIGIN      
        1 aatctacttt attcttatgt tatttagtta ttataatgct ttataaagta aaatattatt
       61 tcgtgtttaa gcttgataac taactcttct tattgataga ctaacaatct tgaataaaaa
      121 tattcctgca actagattta ttaaaacagc tggtaatact actgctaaga ataaagctgt
      181 aaatgaagcc cctggaagac caactatagc ttgagctgct aataaaaaga tagttccact
      241 tacaagagtt cctattggca ttattattgc aactactaca gtatcttgtg ttttcttagc
      301 taattttttt ataaaaggca tattataact aatattcatt aataaaaata ctatatttgc
      361 tgtaagagtt ttatctatta cattaggaat ttgacctccc ggaaaacttg atgtaagtgc
      421 tgtaaatatt cctgttacta tacccgctat caaacaagtt ttataatcat ctctatttaa
      481 aaccaatatt acaaataaca ttgctagtgg tacatctggc ttcattggta atcctatagc
      541 tggtgtgatt tgatgtatta ataatcccat cgctaaaagt attgcattta aagttaattt
      601 ttttgtattt gttttcattt tttgtacttc ctcctatttt tttaattttc aatttatttt
      661 tttgccaacc tactatatca tcatctttag cttttgaaat tcataacatc tttctcacca
      721 ccttctatct tttaaatttt cagtaaaata aaaaactccg tcctaaacta ataggacgga
      781 gattatccgc gttgccacct aaattatata aaaattaata taaaattcaa ttttcataca
      841 tctcattatc aggtacttac ataccttatt cgctataacg tgcgactgac gataaagtct
      901 actctcaaaa ctatgatttc aatttattcc tctaagggcc attcatcaaa ttttttcttg
      961 ctaagattcc accatcctca gctctctata ctgaacctaa atttaactac tcttccttgt
     1021 cataggattt aatttattgt attgaatttt gttgttaaaa taatgatatt cttttttcta
     1081 aaaaatgtca ataccttttt ataaaaaata tattttgtaa tctatttttt ataaaaaaag
     1141 taaattatac tatacatttc ttttcatata caaaattcta tgaataatta ctttaatggt
     1201 ctaatagtct gctctttgac tctaattctt cttatattaa ttttgtgaat aactgttgat
     1261 gtaattttat attttgattc atgaggaatt aaattcttca aagttaatta agtacattta
     1321 tcaataaatt tacttaatta accatcattt aatactcatt cctgtattta tatgcctcct
     1381 ttgaaacaga atattactcc attttaatta catcatatta ttaaatagat accaatgatt
     1441 aaaaaaatga tattctttta attattttta gtaaaatttt ttcttctttc ttttaataaa
     1501 tattagcact actaaaacta taaccaaaaa tatgactatt atggacaata ttaattttgt
     1561 taggatatta tgtcttcttg tgttttgtct tttatattta tttatagttt gatatccata
     1621 ctttttagtt cttatacttc gttttttcaa ctttacttta cttttattat atttcctact
     1681 tttatgtcta cttttagatt taatagtttt aaatttagta ttatcatatg tataattctc
     1741 ttttattgaa gaaatgtata cactagactc aataatagct tctgcttcta cttgtaatgg
     1801 atttataatg ctgattataa gtagaaaaga tatagctatc ccaatcaatt ttttcttctt
     1861 attcatattt tccccctcat aaaaattttt aactagttca ttataatata tatataacta
     1921 aaaattacaa atttttcaag tttcttatat atcttattat ctttaatatt atttttatta
     1981 tcataacttt tacttttttt aagatttttc aaagtagtat taatttatga tacttggtgt
     2041 ataaagtata agttaaatct actttcaatt aaatttacca tttataatta ctttataata
     2101 aaaataaatt agttaattta ttctctttag ttctgttata atgtttctac aaaaaagaat
     2161 cccattaatg ggattctttt ttgcgattta ttaatatatt taaagcttgc tagtatcgta
     2221 ttataaactt aaatgctctt ctatgtattg attatataca tatccagaat atcgtttttc
     2281 atttttattg atattaactc ttgcccattt tccaacgcta taacatactt caactttaaa
     2341 accatcaggg attgtagtta taactttaga ttctaatgtt ctatcatttc taagttctaa
     2401 acttaatcca tttgttctta tatttcctat gtaattcttt tttctatttt ttattgtatc
     2461 attactattg tttttagcat tacttaatct ttctaaatat ttataaagta accaaccttt
     2521 gtcataatta ctctcaactt ctgcccatat tccatctaca cactttaact taacaatttt
     2581 accttctggt atacttttta atttcaaact atttatgtct ggtttttctc ttaaattcaa
     2641 tttaatttta gtcttaaaat tgatgctaga tttacgaaaa ttttttaaca ttaattcaga
     2701 tatcatctta agccctcctc ttgccaaata ttaagttatt aaaatatata actctaaaaa
     2761 tacttttaac aaaaagaatt attatctttt tggcaactgg ctgacatgac ctttattaca
     2821 taaagatttt agtccctata gttttgcgtc attagattta tctaatattg cctatatctg
     2881 aataataaca taatttgaac aattaaaaaa taatattacc tcaaatttta tattatttaa
     2941 atataaatta aatttttaat taataataat caaaaaatat ctttatattt attcaattac
     3001 tgttctttat agtcaatata aaagacagta atttatctat agtgttactt atattttata
     3061 tatatggcta tttttcaaca ttttacaata tatataacat ttattttttt aacttttgtc
     3121 agtaaaatgt cagcaaaaca ctttatccac aatttttata taagttttcc acaacttatc
     3181 cccagtatta tccacataaa aaaggtagct cttatattat tagctacctt ttatttatta
     3241 atcttcatat ttccagcttt ttatccaact tcctatttct attatttttt cttcagttat
     3301 ttcttctcca tcatgtaatt ttgaaattaa ttctgataaa ggattgttat gtaaacctct
     3361 aaaaactctt ttttcctcta ttttgatata ttctctctct tttattaata ctgtatagtg
     3421 tacatgcctc ccaattctct atgatattaa aaaacctttt ttggttaatc tagctaaaag
     3481 tataattgta gttgtatgct tccaaccata tgttttttct atttcatctg caacatcttt
     3541 tgatattaca gttttataac cagtattcca tatgcatttc ataattttta attctgtatc
     3601 tggtaatttt ttatttagca taatatattc cttctttcaa tttatgtcac ttcttcttat
     3661 gtcacttctt caaatttatc tagcttaaat tataatagga gctttttaga acaaaaacta
     3721 tatttaattt ttaccaggta ctctacgtgt ttatcctgtt tttaagtgta aaataatgtt
     3781 catatctata aatttattta actttcaaac aatcctgttg aataataact aattctattg
     3841 tattatttta gttgtagaat aaaactaaat cggcaaaact agagaaattt agtgacgcaa
     3901 agctataggg actaagactt atataaatat cttatgagtt atgtcagcca gttgccaaag
     3961 agatagtgtt ctttttgttt ttatgaaagt tttttagggg gataataatt tatatgtttg
     4021 gaaataaaat ggataaatgt acacatgtat tgactgctta tattagcagt tcgtatgatt
     4081 attgtaattt tttagataca caattagatg attttatatt agagtacgga gaaagtgtag
     4141 tagagtcttg tttacaccaa gtgatggtat tggtaagtaa gtataattag atagacattg
     4201 atttctagag aacccttttt agttttatct acatttatat taatatatag caatgtaaaa
     4261 cgtttaaata tatttttaat attaagaata actaaaatgt taactaaaaa atataattaa
     4321 gtagacattt ctattaatct atgatacaat agtatttgtg gtaatacaca attaaatcaa
     4381 gtatatcttg atataggtta ataggagtat ttttaatgaa taatggaata gtgaaatggt
     4441 ttaataatga aaaaggattc ggttttatat caatggaagg tagagatgat gtgtttgctc
     4501 atttctcagc tatacaaact tcaggattta aatcattaga agaaggtcaa caagtaagct
     4561 ttgatatagt taaaggtgct agaggtcctc aagcagaaaa tataactata ttataatata
     4621 tagatttttc gggaaagact ctttttagag tcttttttta ttgttaatat atgtaagata
     4681 atcattggtg aagtaaataa ggtatattga gtaatgttct tgatagttta tatgctaatg
     4741 aatttcaaaa aaataagcac tcctataaaa agagtgctct taatgtaaat ataaaccttc
     4801 attggtccag cctaataccc cgtattctcc aagttctaac tcttttttat atacattatc
     4861 actttcagcc atatctaaca caatcatttc ttctttagaa tcaatttgta aaatatgagg
     4921 actgtgtgtt gtgacaatta tttgtgtttt tgggagaatt gtttttaatg taataaaaat
     4981 tttacatacc acatagttga tataaaacgt aaatacacca tctataaagg gcaggtgtta
     5041 aaactcttta cataccacat agttgatata aaacttaata tacttaggta ttcctactgg
     5101 aaacttttct ctttacatac cacatagttg atataaaacc ccaaaataaa cttagcatct
     5161 ccaatgttta cacatataca atgctcttaa aattgcagtg aacgcgcaat agtgcaattg
     5221 ataatactta ttatatattc tcaatgcctt atatttcaac tgctaaacct caatttgtca
     5281 caaatatcgc tcactgcaaa atttctatat ttttattata tcataacata taattaatat
     5341 tttcaattaa tttttacatt taattatgtt taaattctat aataatgcaa ctttaatgtt
     5401 tctttctgga actaatataa aaactatttc agaaagatta ggtcatgcag atataaaaat
     5461 aactatgaat agctactctc agtttttaga agagatggac agagaagcat ttgaaaattt
     5521 gagcaaaata ctatttatta tattttaaag aagatgactt attcaatatt tgatgctaga
     5581 atataattaa taaaaattta attataggtg attgtattat gaatattatt aaaagcataa
     5641 ctttactctt aacattatta attgtgatat taatgacagg ctgttcccca agagaaagtg
     5701 ctagtgaagt tgcaaaaaaa tatattgata agatgaactc tggtgactat gaaacagcat
     5761 atagtttatt aagtaaagat agtaaaaaaa aaattagtat agaaaatctt gaagaatttc
     5821 aaaatatttt agttagtaca aaaagaataa ttagttataa aataggtaaa gaaaaaatca
     5881 taaaaaaata taaacatgat ggaaaagaat ataaaaatgt tgttaaatta gaagaaactt
     5941 ttaatataaa aaaccttctt tataaaaaag atgattctct aaaagtaaat agatattttg
     6001 tagttgaaaa taacaaatac aagctattat tgtatgaaag tttcaaaaaa gaatttggta
     6061 ttaatagtat ggaccttgct caacttaagc ttaatgattt tggactaaaa tactttaaag
     6121 aagttgacct tatactaaaa aaagctattg ctattaatcc tactgattca aagctttatt
     6181 atgcccaagc atgtaattat atgaattttg atggatttcc tggttattca gatacaacat
     6241 ataatgcttt agattcaatt aataaatctt taaagtattt agataaaaat gattctgact
     6301 acaaaaaaaa tgcaagtaat atatataact taaaaggagt tattcttatg tttcacaaaa
     6361 gatttgatga agcacaacaa tatttaaata agtccttata ctttaacaaa gataatgaag
     6421 acccaaaata taatctaaat acaataaaaa aacatctaaa ctagatattt aatttttgaa
     6481 tttataaaat atttttttat gtataattca gcactgaatc ataatctcta ctaaactaat
     6541 tacaagttta aattataaag acttatttct atataaatat tactctataa caataagttt
     6601 cttatttaaa ttttaaaata ttatctaaaa atcataaaaa agaaggtata aagagctacc
     6661 ctcaagctca atatacctta taaatatcta aacttcattt tttactaatt tttctatatc
     6721 tttgataata tgttctttag atttttttcc agctatggca tcaaaaattg caacagcctt
     6781 tttctctcca aacttttctg ctacatcatt aaagtctaaa aatctaacac tttcttttgg
     6841 tttaccaaac atataacacc tctcatacat ttttatatat ttattataac ttctatagac
     6901 ttatttttca ccaataaacg ttagacaaat tagtcccttt atttttttaa actagtttta
     6961 ccttcaaaat tcttaacaaa aacagtttat tctactctat aaataaaaat agacaattta
     7021 atttgtagga aactgataat agaatacagt aaataaaact ttgtttaatc tatctgaaaa
     7081 agttatctat taaaactgct atgaatttat aaagaatatt ctagttcata aaaatacaaa
     7141 tatgtaaata taatcacctt aatattcaat ttatttaaaa taaaaaatgt ataaaagatt
     7201 taataatgac aatatactat tctcaataca atctatgatg taaataattt aaaactacta
     7261 tattatttaa aaatttatat ttatatatgc aattctaaaa acttaataag aagtgaactt
     7321 agaaaataaa gcttaatttc aaagacttaa atagactatt attattagtc taaataaact
     7381 acgtctatca aaattatata ttaaattctt ctaaaatttt tattgcaaat cttgcgtttg
     7441 gatatgtaat agacccgcca ccaattacac ctatttttat agcttctata atttcatctt
     7501 tacttgctcc agaattaata caaccctcaa tatgataaca aatacactca tcacatcggc
     7561 ttaaaattga aatagctagt cccattaatt ccttattttt tttacttata gccccttctg
     7621 aataagtctt atcatcaaga cttccaaata ctctaaaaaa ctcatctgtt tcgcttaatt
     7681 ttttattgta aaatacccta tattctttca tttctctagt attcatcttc tacctcctat
     7741 gtttttctta taagaatatc acactgtact tatattttca atattaaata caaattttca
     7801 ttttatgttc attagcctaa tttatattat tttttgaaat ctcaataagt tatcatataa
     7861 gtaagatttt ttcagaacta aaatagaaat cttagtaatt tctttattac ctatctcagt
     7921 tctgaaatat attattctta tc
//
LOCUS       DAELLV010000169         7895 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.169, whole genome shotgun sequence.
ACCESSION   DAELLV010000169 DAELLV010000000
VERSION     DAELLV010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7895
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.169"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(392..1216)
                     /locus_tag="KPD58_002785"
     CDS             complement(392..1216)
                     /locus_tag="KPD58_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG0266520.1"
                     /translation="MRTEKSLKRPIASFIILTNIIFLPLFLLVVVTIMLKLPTVISDI
                     ALCISAWSSTFAFMILFKKIYPDKKFLVHVKDRFRNKLKFSTVSIIISIQVLIAVVVI
                     FILASKNHGIMPNFTVSSWGAFIYLFLKNLFAGSLGEELGWRGFVQNHLQKRHSPLKA
                     SIIVGFWWGMWHLPIWFTTGFKGLDLIKYIIFFMIAIISISIIMTTFYNLNKTLIIPI
                     VIHQLFNFLIGIINGDLIELINYYAMLYLIVAIVLVAINPGNALYKRFSKNTTQIL"
     gene            complement(1320..2114)
                     /locus_tag="KPD58_002786"
     CDS             complement(1320..2114)
                     /locus_tag="KPD58_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0266521.1"
                     /translation="MYTIGQVSKFLGISRDTLKFYEDKGLVNPKKNDENGYRIYNQVD
                     IYDIATINFYREIDIEIKKIQEIRKSKSINNLELLLEEKEQIILKEIEYKKLLLKKVQ
                     LVKEDCDKIKTYFGKYTIKEMKPLEVKGKIKHVTNYEEYEALKENTNNLKKAVTLTDL
                     RRVINFNEEGIIEEKFIIVREVDDYNKIIEGEVLSHPNCIYTIIEDGRWSTGGENIDN
                     KVEDRLREIAIENECELLGIVYVNILLTTYEEGIERIFLEIYAPIK"
     gene            complement(2648..3502)
                     /locus_tag="KPD58_002787"
     CDS             complement(2648..3502)
                     /locus_tag="KPD58_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HBG0266522.1"
                     /translation="MAMGFEFKIMRSLIYVGLAKEEYRPKLMDWLYRHHIPDSISTFG
                     PYCTKYAFYQAYPTPNEGERFGARKMQLTEHYWLVDEHMPEMANRIMTEYMPMDVLRW
                     QGCIPDVENKRVHENVESGDAGRAVGGDNGCPPFIFAFVPINWEEDFRGKGRTVQDGP
                     NYRWQFMIKYPDGISKEEGEKWFYDEVVPYFTNCCYVNRFVSSKIMINYGATAFDRVS
                     ELWFEGEEEWYKAVVEETKSFIKKPEWAQEEEFPYLKPQFNIASVFLGDIATMDAYSQ
                     YRGYIPMR"
     gene            complement(3534..4046)
                     /locus_tag="KPD58_002788"
     CDS             complement(3534..4046)
                     /locus_tag="KPD58_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBG0266523.1"
                     /translation="MDRKKKEILTLSMGIALLPPLWAVLAPYIGIKTGAVALICAGLY
                     VTNGNKQKDGLKIMFGFWCGDLWAVLAILIMGYMNFNQNLELFLTLSILGFFAVVIAS
                     LFEKIIFLPSWLCGWAIGLTVMTGENIINLQDICIQIAVAMAVGVWYVGAGVDLFTIY
                     ILSKDRKKGN"
     gene            complement(4039..4263)
                     /locus_tag="KPD58_002789"
     CDS             complement(4039..4263)
                     /locus_tag="KPD58_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266524.1"
                     /translation="MNEKPSFKQIEVILAVIFLVLTYYKVRLTPAFIVIFIPLYLSYF
                     AVSMIVNQIAIKLLEIRQAREATNYNIGNG"
     gene            complement(4323..5276)
                     /locus_tag="KPD58_002790"
     CDS             complement(4323..5276)
                     /locus_tag="KPD58_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBG0266525.1"
                     /translation="MKILGISFGTKNGNNDTMCKVALKGAKEAGAEVEFIQMSSINIK
                     HCTGCCACVKTLLSGKGSMCVLKDDFEWLLDKMKDADGIVVSDPIFEEGASGLFHTIM
                     DRFGPRADRGNNIIGTKVAEVIGGKIPDSRMLNDKVISFMGIGGSDWGTRVQCEHAML
                     ALIPMWKVIDNAWFPWAKELVMDDTRLVQVHQIGLNIVEAAKDFEKASYQGEEGVCPH
                     CHNRLFYLEPGTKKAICALCGIVGELDTISGKTIFSFPEEQLGHAHDTLPGKFIHGDD
                     IKKMEGHYAEVRKTQEFKERKTSYNFIEPLTKEKQAGVSLG"
     gene            complement(5304..6692)
                     /locus_tag="KPD58_002791"
     CDS             complement(5304..6692)
                     /locus_tag="KPD58_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266526.1"
                     /translation="MEDKFYAKGNGNNGYIKNLEVCSFNNLDGTCGMFQMALYKRDEK
                     YYLYGCCFGGNKKNGVMISDITDPYNPQFIKHFQMLDPKEYPTTTTPKIQIADDLMIV
                     AMSCGSGPGALVDQAKLANIKCEAGIRIYSLKEDPLNPKFLGYWDCGLKHVMGVHRFM
                     YNGGRYVHLSSDCVGFEGLIYRVIDIINPTNPVEIGKWWRPDQYADGYPNRTFDAGAP
                     HCPEFMDKGWLHGPPFVRDGKAYCGYGGAGLVVLDVEDLTRPRCLGELPFTPAFSSRL
                     AGARTHTALPLPGRDLVVVQNEGERFQFFKPDNITDVQAMNNIHMVDVSDPTKPTLIA
                     QFPYPEVPKDFPYPNFNVAGLGKPGPFGPHNLHEPMDNKPWLEQRGDRVYCCYFHAGL
                     RVYDVSDPYYIKELAYFIPPNPNKTPEESYFPGFPGPRLAVTEDLIVDDRGYIIIDAL
                     DDGFYILKMKED"
     gene            6966..7847
                     /locus_tag="KPD58_002792"
     CDS             6966..7847
                     /locus_tag="KPD58_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG0266527.1"
                     /translation="MNTKQLEYFISVAEKLSFTKTAEKFYISQTAVTQQIKALEEQIN
                     VTLFTRSKRHVELTPAGKVFLSEARTIIKNINDAIAKTQQFAHGFFGTLSIGTLIGYE
                     KNKLQQYLKEFSNTYPNISMDICTNEITELLNLVKNNSMDLAFVINPENQPLKDFEYK
                     TVERYSLVALLPPGHPLYNEDSIDLIELQHDKFIFVKETGDEYGQKSMIQNRYREAGF
                     VPNVVQRSNNLNTIESLVSSNMGISILPSFCVTDTMIERDIAIVPIKEVENRIEVVVV
                     WNKKNTNPALEKFISII"
BASE COUNT         2617 a         1403 c          909 g         2966 t
ORIGIN      
        1 atcaaaacga tactaattat gcataaatta ccataaataa aaggcacatt aatttaaaat
       61 aaaattaatg tgccttattt ataattctaa tatattaaat ttatttttta acttgtagtt
      121 taatatcctt tttcttcaat taattcatat aaataaagta tttaataata aatgttcctt
      181 ataattttaa tattttaaac caagaaagtt aaagtttctc tcatataata agttgatact
      241 tatctatttc attcttttaa gtgtttcatc taatgaaata cactctgcat accttccatt
      301 ccagataaat ctattgtaat atttattaat taaaattaat taataaggag aattatttct
      361 ctcatttgtg atatgattct cacttatatt attaaagtat ttgtgttgtg tttttagaaa
      421 atcttttata taaggcattt cctggattta tagctaccaa tacaattgca actattaaat
      481 ataacattgc atagtaattt ataagctcta ttaaatctcc attaattatc cctattaaaa
      541 aattaaatag ttgatgtatt acaataggta ttatcaaagt tttatttaga ttataaaaag
      601 ttgtcattat aatactgatt gatataattg caatcataaa aaatataata tattttatta
      661 aatcaagtcc tttgaatcct gttgtaaacc atattggtaa atgccacatc ccccaccaaa
      721 aacctactat tatggatgct tttaaaggag aatgtctttt ctgaagatga ttttgaacaa
      781 atcctctcca acctaattcc tcacctaatg atcctgcaaa taaatttttt aaaaataaat
      841 atataaatgc tccccatgat gaaactgtaa agttaggcat tattccatga tttttagatg
      901 ctaatataaa tataactact acagctatta ggacttgtat actaattatt atagatactg
      961 ttgaaaattt aagtttattt ctaaatctat cttttacgtg tactaaaaat ttcttatctg
     1021 gataaatttt tttaaataat atcataaatg caaaagtgga tgaccacgct gatatacaca
     1081 acgcaatgtc agatatcact gttggaagtt ttaacattat agtcactact accaataaaa
     1141 aaagtggtaa aaaaattata tttgttagaa taataaaact ggctataggc ctttttaatg
     1201 atttctctgt tctcataagt aatcctcatt tcaaattaat atttaattgc tatataagtt
     1261 ataactgctt atatcatgag tttaaaccta tgagtaagtc ccaggtcaat gcttttatat
     1321 tattttatag gtgcataaat ctctaaaaat atacgctcaa tcccttcttc ataagtagta
     1381 agcaatatat taacatagac tattccaagt aattcacatt cattttctat tgctatttct
     1441 cttaatctgt cttccacttt attatcaata ttttctcctc cagttgacca tctgccatct
     1501 tcaataatag tataaataca attgggatga gatagaactt caccttctat gattttgttg
     1561 tagtcatcta cttcccttac aattataaat ttttcttcta ttataccttc ttcattaaaa
     1621 tttattactc ttcttaaatc agttaatgta actgcttttt ttaagttgtt tgtattctct
     1681 tttagggctt catattcctc atagttagta acatgtttta ttttaccttt tacttccaaa
     1741 ggcttcattt cttttattgt gtattttcca aaatatgttt ttattttgtc acaatcttct
     1801 ttgactaact gaaccttttt taagagaagc tttttatatt ctatctcttt tagtatgatt
     1861 tgttccttct cttctagtaa gagttctaaa ttgtttatac tcttactttt tcttatttct
     1921 tgaatctttt taatttctat atcaatttct ctataaaagt ttattgttgc aatatcatag
     1981 atatcaacct gattatatat tctatatcca ttttcatcat ttttcttagg atttactaat
     2041 cctttgtcct cataaaattt tagtgtatct ctagatattc ctaaaaattt agatacttgt
     2101 ccaattgtat acattatatt ctcctattat tagataaatt ctttatgctc attaaacgta
     2161 tttctattaa caattacaaa atcacctaaa ctttgaagta tcaaagtcag catagtattt
     2221 acaacctttg gaattactct ttttatttca taatccaagt gtaaacttgt taatatgata
     2281 cgttataaag actatattgt ctacatattt ctaacaatga ttctttcact ttaagatgtt
     2341 cttaaacata tttaactttt atttttaaaa atttcctata cataaaaata ttcctttcat
     2401 gataacagtt ttattatttc aactatctgc taccataaaa ggaatatctt aatctttaaa
     2461 cacattctaa atgctctttt aacaaatatt atcaaattat tctaattcta ttgtttcata
     2521 tttattagtc ttagttttat ataaatttaa atataaaact atatattcac ctacttttaa
     2581 ggatacaata agtcttcttc taccagtaag agtaaatgcc tcctctactt atcacaaact
     2641 ataattttta tctcattggt atatatccac gatactgtga gtatgcatcc atagttgcta
     2701 tatcacctaa gaatactgat gcgatattga attgtggttt taaatatggg aactcctctt
     2761 cttgtgccca ttctggtttt ttaataaacg actttgtctc ttcaaccaca gctttatacc
     2821 attcttcttc cccttcaaac catagttctg atacacggtc aaaagcagtt gctccataat
     2881 taatcattat tttactactg acaaaacgat taacatagca acagtttgta aagtatggca
     2941 ctacctcatc atagaaccat ttttctcctt cttctttaga gataccatct ggatacttaa
     3001 tcataaattg ccaacgatag tttggtccat cttgtacagt acgtcctttt cctctaaagt
     3061 cttcttccca gtttattgga acaaaggcaa atataaatgg tggacatcca ttatctccac
     3121 ctactgcacg tcctgcatct ccactttcta cattttcatg aaccctttta ttttctacat
     3181 ctggtataca cccttgccaa cgtagaacat ccataggcat atattctgtc ataattctat
     3241 ttgccatctc aggcatatgt tcatctacaa gccaataatg ttctgttagt tgcatcttac
     3301 gtgcaccaaa acgctcacct tcatttggtg taggatatgc ttgataaaag gcatatttag
     3361 tacaatatgg tccaaaagtg ctaatactat ctggaatatg gtgacgatat aaccagtcca
     3421 ttagcttagg tctatattct tccttggcaa gtcctacata tattaaactt ctcattattt
     3481 taaattcaaa acccatagcc attctcctat aatcaaagta tattcacatg ctttcaattt
     3541 ccctttttcc tatctttact caaaatatat attgtaaaca aatctacacc tgcacccacg
     3601 taccatactc ctactgccat agctacagca atttgaatac aaatatcttg taaattaata
     3661 atattttcac ctgtcataac agttagacct atagcccaac cacaaagcca tgatggtagg
     3721 aaaattattt tttcaaataa ggatgcaatt acaactgcaa aaaaacctaa tatgctaagt
     3781 gttaaaaata attctaggtt ttgattgaaa ttcatatatc ccataattaa aatggctaac
     3841 actgcccata aatctccaca ccaaaaacca aacattattt ttaatccatc tttttgttta
     3901 ttaccattag ttacatataa tcctgcacag attagtgcta cagctccagt ttttattcct
     3961 atataaggtg ctaatactgc ccataaagga ggtagtaaag caatccccat agacagtgtt
     4021 aatatttctt ttttctttct atccatttcc tatattgtaa ttagtagctt ccctagcttg
     4081 ccttatctcc aatagcttaa ttgctatttg atttacaatc atagatactg caaaataact
     4141 taaatatagt ggtataaata taacaataaa tgctggagtt aatctaacct tgtaatatgt
     4201 aagtaccaaa aaaataactg ctaaaataac ttcaatttgt ttaaaacttg gcttttcatt
     4261 catacaataa gcctcctata acatctaaca tcccttaaaa aagagtctta gtattttaat
     4321 tattatccta atgaaactcc agcttgcttt tctttagtta atggctctat aaaattataa
     4381 gatgtttttc tttctttaaa ttcttgtgtt ttacgaactt ctgcataatg accttccatt
     4441 ttcttaatat catcaccatg tataaactta cctggtaaag tatcatgagc atgacctaat
     4501 tgctcttctg gaaaactaaa tatagttttt ccacttatag tatctaactc tccaactata
     4561 ccacatagag cacaaattgc ctttttagta cctggctcaa ggtagaataa tctattgtgg
     4621 caatgtggac atacaccttc ttctccttga tatgaagctt tttcaaaatc ttttgctgct
     4681 tcaactatat ttaaaccaat ttgatgaact tgaacaagcc ttgtatcatc cattactaat
     4741 tcctttgccc atgggaacca agcattatct ataactttcc acattggtat gagagctagc
     4801 atagcatgtt cacattgaac tcttgtaccc cagtcagaac ctcctattcc cataaatgat
     4861 attactttat cattaagcat tcttgaatct ggaatttttc ctcctataac ttctgcaact
     4921 tttgttccta taatgttatt tcctctatct gctctgggtc caaaacggtc cattatagta
     4981 tggaataatc ctgatgcccc ttcttcaaaa atagggtctg aaactactat accatctgca
     5041 tctttcattt tgtcaagcaa ccattcaaaa tcatctttta atacacacat actacctttc
     5101 ccagataata aagttttaac acacgcacaa catccagtac aatgtttaat gttaatagat
     5161 gacatttgaa taaattctac ttcagcccct gcttctttgg cacctttaag tgcaacttta
     5221 cacattgtgt cattatttcc attttttgtt ccaaaagaaa tacctagtat tttcattttc
     5281 aactctccct ctttagtatt tatttaatct tctttcattt ttaatatata gaatccatca
     5341 tctaaagcat ctatgatgat gtagcctcta tcatcaacga taagatcttc tgttactgcc
     5401 aagcgtggtc ctgggaatcc tgggaaataa gattcttctg gtgttttatt tggatttggt
     5461 ggtataaaat atgctagctc tttgatataa tatgggtctg atacatcata aacccttagc
     5521 cctgcatgga aataacagca atatactcta tctcctcttt gctctaacca tggcttatta
     5581 tccattggtt catgaagatt atgtggacca aatggccctg gttttcctaa tcctgcaaca
     5641 ttaaagttag gataagggaa atcttttgga acttcaggat atggaaattg agcaattaat
     5701 gttggttttg ttgggtcact aacatcaacc atatgtatat tattcatagc ttgaacatct
     5761 gtaatgttat ctggtttaaa gaactggaat ctttctccct cattttgaac aacgactaaa
     5821 tctcttcctg gcaatggtaa tgctgtatga gttcttgcac ctgcaagtct actagaaaat
     5881 gcaggcgtaa atggcaattc acctaagcat cttggtcttg ttaaatcttc aacatctaat
     5941 acaactaaac cagctcctcc ataaccacaa tatgcttttc cgtctcttac aaatggaggt
     6001 ccatgaagcc atcctttatc cataaattct gggcaatgag gtgctcctgc atcaaaagtt
     6061 ctatttggat atccatctgc atattggtct ggtctccacc atttacctat ttccactgga
     6121 ttagtaggat ttattatatc tatgacccta taaatcagac cttcaaagcc aacacaatca
     6181 cttgataaat gtacatatct tccaccattg tacatgaatc tatgaacacc cattacatgc
     6241 tttaagccac aatcccaata tcctaaaaac ttaggattta aagggtcttc ttttaaactg
     6301 tatattctaa ttcctgcttc acacttaata tttgctaatt tagcttggtc aacaagtgct
     6361 cctggtccac ttccacaact cattgctact atcattaaat catctgctat ttgaattttg
     6421 ggagttgttg ttgtaggata ctctttaggg tctaacattt gaaaatgttt tataaattgt
     6481 ggattataag ggtctgtaat atcgctaatc attactccat tttttttatt tcctccaaaa
     6541 cagcatccat ataaatagta tttttcatct cttttgtaca gagccatttg aaacattcca
     6601 caagttccat ctaagttatt aaaggaacaa acttcaagat ttttaatata tccgttattt
     6661 ccgttgcctt ttgcataaaa tttatcttcc attttttctc ccttcttatt ttaaagtatt
     6721 atcaattgat atactttcat tttatgagac aaacttactt aaatccaata tcgttttcat
     6781 tggaatatat aagttattct tattaataaa tttataaaat ctaatgtaaa gatatactta
     6841 aaacatatat ttttatacaa atattttcta atattcttta aaagtaaatt ttcttatact
     6901 ataataagct ttaattatag ataaataagg tttacaaaca atcaagttta aataggggtg
     6961 attatttgaa tactaaacaa ttagaatatt ttatatctgt agcagaaaag ctaagtttta
     7021 ccaaaacagc agaaaaattt tatatttctc aaactgctgt tacacagcaa ataaaagctt
     7081 tagaagaaca aataaatgta actctattta caagaagtaa acgtcatgtt gaactaacac
     7141 cagctggaaa agttttttta tctgaggcac gtactataat aaaaaatatc aatgatgcta
     7201 ttgcaaaaac acaacaattt gcacatggtt tttttggaac tttgagcatt ggaaccttaa
     7261 taggatatga aaaaaacaaa cttcaacaat atttaaaaga attttctaat acatacccta
     7321 atatatctat ggatatttgt actaatgaaa taaccgaact tttaaactta gtaaaaaata
     7381 attctatgga cctcgctttt gttattaatc cagaaaatca accattaaaa gattttgaat
     7441 ataaaactgt agaaagatat tctttggtag ctcttttacc tcctggtcat ccactttaca
     7501 atgaggattc tatcgattta attgaattac aacatgataa atttattttt gtaaaggaaa
     7561 caggggatga gtatggacaa aaatctatga tacaaaatag atatcgtgaa gcagggtttg
     7621 tacctaatgt ggtacaacgt tcaaataatc ttaatactat tgaatcattg gtatcttcta
     7681 acatgggaat atccatttta ccttcatttt gtgttacaga tacaatgata gaacgtgata
     7741 ttgcaatagt acctataaaa gaagtagaaa atagaattga agttgtagtt gtatggaata
     7801 aaaaaaatac aaatccagca ttagaaaagt tcatttctat aatataaaat taatcaatat
     7861 taacgataaa aatagcatta accatttttt ctata
//
LOCUS       DAELLV010000170         7859 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.170, whole genome shotgun sequence.
ACCESSION   DAELLV010000170 DAELLV010000000
VERSION     DAELLV010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7859
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.170"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..1842)
                     /locus_tag="KPD58_002793"
     CDS             complement(<1..1842)
                     /locus_tag="KPD58_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266528.1"
                     /translation="MKKYEIFIFMSIIVLCFISFPHKVFGQEENCLEDFERWVQENKN
                     NKEEIVYTLSCDMVIDEEFRFYIPYDSNFTIDTNKYKILIKNHGRFIIDDNELNITGE
                     GGKEGVIHIENGGSISIGINNIIATDGTALYVEEGGDLHIKSSYESVEKIKANGKDAI
                     GIYTENDIRILNKNIEVNGEHAIGVYSKGDVEIEETSIKAYSSNNESGLLKDNNKLAQ
                     SIISESKKVYIIDDYNELIPSIAEDSGYDIVKCCHRGIDVFSDEIKVSKDDKIENIKF
                     PKYMVLETSSGNRLDIDVEWDFTDYYEKLEKGEDFNITGKFKTEMLNKEKIIINDDVV
                     PSLNVSVIEKKPIDNLELEFQNTKNGYVAVLFYDMPYSASKVFVEYSSDGINWISEEQ
                     EDIRDQAILFFEDFKLRCFRVKVVGGLKEGYSNIVFKPGFIMGGGDNQETPDDEREND
                     DIDGDRGGGGRDDPDRDEDDNNQDGNNGNENNDSNNNQNNNNENNNNENDDSDEKQPN
                     TPKDDVESSTDSNENKNPHDIPKDGNKENNSDKEKGFYNKNSITENDDNYDLSSKSVF
                     GTNEHSFPYRESISNKNQDTNKNKSNISDYDGIINNNYKLESKEYNLK"
     gene            complement(2025..3500)
                     /gene="spoIVA"
                     /locus_tag="KPD58_002794"
     CDS             complement(2025..3500)
                     /gene="spoIVA"
                     /locus_tag="KPD58_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HBG0266529.1"
                     /translation="MNNNIYEDISKRTQGDIYIGVVGPVRTGKSTFIRKFMEKLVIPN
                     IDNEFKKDRTRDEIPQSGSGKTIMTVEPKFVPADGVEIKIKDTVSLKVRMVDCVGYIV
                     EGALGHEEGGKQRLVSTPWSQEAMTFEKAAEIGTKKVIKDHSTIGIVVLTDGSVTGID
                     RKSYVEPEERVIQELKNLKKPFAVVLNTLSPKSEETSMLRSELEEKYEVPVLPMNVVE
                     MEEEDIEEVMEAVLYDFPLTEIRINLPKWVEGLERNHWIKSSIITTLKQSIIDIGKIR
                     DIEGIIQGFSELEFLEDTGVDNVELGEGVINIDLQTKQDLFYNVLEEKSGFKIEGDYQ
                     LLSLITRLSKVKNEYDKIESALIDAKIKGYGVVAPSLEELSLEEPEIMKQGKQYGIKL
                     KANAPSLHIIKADISTEVSPIVGNQNQGEEMIKYLMEVFEEQPADLWESNMFGKSLHD
                     LVKEQLQSKLYTMPEEIRVKMQKTLQKIVNEGSSNIITILL"
     gene            complement(3672..4966)
                     /locus_tag="KPD58_002795"
                     /pseudo
     CDS             complement(3672..4966)
                     /locus_tag="KPD58_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903280.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(5150..6559)
                     /locus_tag="KPD58_002796"
     CDS             complement(5150..6559)
                     /locus_tag="KPD58_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG0266530.1"
                     /translation="MKKKIGFWDLVFMNISALFGIRWIAKSTASSFGLGLGSIPVWLL
                     FAFIFFVPASLICAELAATYPKDGGLGEWVKQAYGEKWGFMVSWLNWTSKIFWYSSFL
                     TFLAINIAYMLGNPDLSNNKMFILALSLIIFWLLSLVSTRGMAFGKFFTNTGALGSTI
                     PAILLIVMAFMSVVILKKAPSASIYTIQNIIPKIDANSLVSISAIIFALSGAETTANF
                     ITEMDNAKKNFPKAILTVAVLIGGIYILGSVAITMILPPDKIAASTGILDALAKVAQD
                     LGIGSWFIRIVAFGITLSVLGALILYIAGPVKMLFGNVREGVFPKQLTVTNKYNIPSN
                     AVIVQAIIVSLLLVGTNLLPSVDNIYNVLVTMTALTALFPYVLLFTSYIRLRKTRPNE
                     ERPYSIAKSDSVCINIARMVLVVTVVGILFSTAPVMETLKDNIIYEIEMIGGGLFVII
                     TGLMLWKNYEKKISNNSGC"
     gene            complement(6611..7750)
                     /locus_tag="KPD58_002797"
     CDS             complement(6611..7750)
                     /locus_tag="KPD58_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG0266531.1"
                     /translation="MSNNMRLERLRKYMNEKSIEIALIFEPDNQFYISGFKAITYSRP
                     IVTVVTQDKIELIVPGLEELHAKEVAKVDNVYVYYEIPEMKEHGISHKHYLDIILNKY
                     PKGTTVGIEKDIVSASFSEYITDKSFAIKDVGSKIFEMRYVKDAKEIEFLKIAGYLSD
                     IGIKGSLENVRVGMSELEFDVAGDNALLKYVSENYPDTYIGFANWTCSGIDRTAQPHL
                     DSNTRILQRGDIVIHSRQVWYENYRAENERTFIIGKPTERQKEVFKIAVEAQQAGLDT
                     IKAGIPARMVDEAARAVVAKYGLELYSNHRIGHGLGLSEHEEPYLRFDNELILEEGMV
                     FSMEPGIYIPGVGGFRHSDTAIVGKNGDTIITNYPRSVEELILDI"
BASE COUNT         2478 a         1463 c          858 g         3060 t
ORIGIN      
        1 ttttagatta tattctttag attctaattt ataattatta tttattattc catcataatc
       61 acttatattt gacttatttt tatttgtatc ttgatttttg ttagaaatac tttccctata
      121 aggaaaagaa tgttcattgg taccaaatac tgattttgag cttaaatcat aattatcatc
      181 attttctgta attgaatttt tattgtaaaa tcctttttct ttatcagaat tattttcttt
      241 attaccgtct ttaggtatat catgaggatt tttattttca ttgctatcag tacttgattc
      301 tacatcatct ttaggagtat ttggttgttt ctcatcagaa tcatcatttt cattattatt
      361 attttcatta ttattgtttt gattgttatt actatcatta ttttcatttc cattattccc
      421 atcttgattg ttatcatcct catctctatc cgggtcgtct ctacctcctc ctccacggtc
      481 accatcaata tcatcatttt cccgttcatc atctggcgtc tcttggttgt ctccgcctcc
      541 cattataaat cctggtttaa aaactatatt tgaataccct tcttttaatc ctcctacaac
      601 ttttactcta aaacaacgca acttaaaatc ttcaaaaaaa agtatagctt gatctctaat
      661 atcctcctgc tcttcagata tccaattgat tccatcgctt gaatattcaa caaaaacctt
      721 acttgcacta tacggcatat cataaaataa aacagctaca tatccatttt ttgtattttg
      781 aaattccagt tccaaattgt ctattggttt tttctctata acagatacat tcaaactagg
      841 aactacatca tcatttatta taattttttc tttatttaac atttctgttt taaactttcc
      901 tgttatatta aaatcttctc ccttttccaa cttttcataa taatcagtaa aatcccattc
      961 tacatctata tcaagtcgat taccactaga tgtttctaat accatatatt tgggaaattt
     1021 tatattttct attttatcat ctttacttac ttttatttca tcactaaata catcaattcc
     1081 cctatgacaa catttcacaa tatcatagcc agaatcttca gctatagagg gtataagctc
     1141 attataatca tcaattatat aaactttctt actttctgat ataattgatt gtgccaactt
     1201 attattatct tttagtaacc cactttcatt gttgctacta tatgctttta ttgaagtttc
     1261 ttctatctca acatcacctt tagaataaac acctatagca tgttctccat tcacttcaat
     1321 attcttattt agtattctaa tatcattttc agtataaatt cctattgcat cttttccatt
     1381 tgcctttatt ttttcaacag attcgtagga tgatttaata tgtaagtctc ctccttcttc
     1441 aacgtataag gctgtcccat cagtagcaat tatgttgttt ataccaatac ttatacttcc
     1501 accattttct atatgtatta ctccttcttt tcctccttca cctgttatat tcaattcatt
     1561 atcatcaata ataaatctcc catgattttt tatcaatatt ttatatttgt tggtatctat
     1621 tgtaaaatta ctgtcataag gaatataaaa tctaaactct tcatctatta ccatatcaca
     1681 agaaagagta taaactattt cttctttatt atttttattt tcttgaaccc acctttcaaa
     1741 atcttcaaga caattttcct cttgtccaaa aactttatgt ggaaaactta taaaacacaa
     1801 aactattata ctcataaaaa taaatatctc atactttttc atcttacact cctagaaatt
     1861 tttagctttg aaacaatcct gtcgaaacat atacttcacc cactataatt tttccacaat
     1921 tatcaattat tgtcaattta tataagtatg tttattaatt gttataaata aaaagctact
     1981 tactaaagca aacagacgtt tcactttaat aagtagctat atttttataa caaaatagtt
     2041 ataatattag aacttccttc atttacaatt ttttgtaaag tcttttgcat tttaactctt
     2101 atctcttctg gcatagtata taatttactt tgtaattgct cttttactaa gtcatgtagt
     2161 gacttaccaa acatatttga ttcccataaa tctgctggtt gctcttcaaa tacttccata
     2221 agatatttta tcatctcttc accttgattt tgatttccaa ctattggaga aacctctgta
     2281 gatatatctg cttttataat atgaagtgat ggtgcattgg cttttaactt gataccatat
     2341 tgttttcctt gtttcattat ttctggttct tccagactaa gttcttctaa ggaaggagct
     2401 actactccat atccttttat cttggcatct attaaagcac tttctatttt atcatactca
     2461 tttttaactt tagatagtct agttatcaga ctaagcagtt gataatctcc ttcaatttta
     2521 aatccacttt tttcttcaag gacattatag aataaatctt gctttgtctg taaatcaata
     2581 tttataacac cttctcctag ctctacatta tcaacacctg tatcttccaa aaactctagc
     2641 tcagaaaatc cttgtataat accttctata tcccttattt tacctatatc tataatactt
     2701 tgttttaatg ttgttattat gctacttttt atccaatgat ttctttcaag accttcaacc
     2761 cattttggta agttgattct tatttctgta agtggaaaat catataatac agcttccata
     2821 acctcctcta tatcttcttc ttccatttca accacattca ttggaagaac tggaacttca
     2881 tacttttctt ccaattcact tctaagcata ctcgtttcct cactttttgg acttaacgta
     2941 tttaaaacca ctgcaaatgg ttttttaaga tttttcaatt cttgtataac tctctcttct
     3001 ggttctacat agctttttct gtcaattcca gtaactgaac catctgtcaa gacaactatc
     3061 cctattgtag aatggtcttt tataactttt ttagttccta tctctgctgc cttttcaaat
     3121 gtcatagctt cttgtgacca aggagtggat actaatcttt gttttccacc ttcctcatgc
     3181 cctaaagctc cttcaactat gtatccaaca caatctacca ttcttacttt taaagatact
     3241 gtgtctttta tttttatttc tacaccatct gctggtacaa attttggttc tactgtcatt
     3301 atcgttttac ctgaaccact ttggggtatc tcatctcttg ttctatcctt tttaaattca
     3361 ttgtctatat taggtataac caacttttcc ataaattttc ttataaaagt tgattttcct
     3421 gttcttacag gtccaactac tccaatatat atatccccct gagtcctttt ggatatatct
     3481 tcgtatatgt tattattcat attattcctc ctatacatct aatcttatta aattatattt
     3541 agagttaagg gttaatattc ttaaaataga aaaataaaga ccagcaatat taatttaatt
     3601 tacatataga aatttgtaaa tttcaatgta aattaaatta atattgctgg tccaaatagt
     3661 agtgtattat attaactgac tttagtagta ttttcatttg aattattatt catctcaata
     3721 agttctttac gtctttcttt tgtcatgttc atccatataa atgtaaacac caatccaatt
     3781 atggctacta tagtaagaac tatgaagtag tacctatatc cagttacacc cggaaatgca
     3841 tccaatatct tccctgctgc taaaggacaa agtacttctg gaagatatcc taatgtagca
     3901 actattccta tagcagttcc tgatattgat aatgaataat taccttcttc aagtaacgaa
     3961 tagtgtaaac cttgaattgc atacatagat acatatattg caacacatcc aatcaatagt
     4021 atataaatca tagatgattt tgttggtgta aatattacac ctaaaagacc tgcaatcata
     4081 agtataaatc cataagatat aatctttgat gaaccaactt tatctccaag taatccagaa
     4141 gcagctgatg ctactggtct acaatactga gctaatattg agattgcaga tgcaaacacc
     4201 acagtagctc caaatgattc agttgaataa ggcgtaaaat aataaaatga catattagct
     4261 gaatatgaac aaaacattat tattgttatt atccatgtat gaggcatttt tattactgca
     4321 aaaaaggctt ctttatcaaa ttttgctgat acctcttttt cattatcctt taatttgaaa
     4381 aatactaata ctccaactat tacatttatt gcgagtataa tactataata gctgtaagac
     4441 cagatttatc acttactttt gatgaaatat acccaaatag tatcaatact agagatagat
     4501 gaattgcatt ggtaattcca cgaccacttt ccataaatcc aaaagctttt ccttgttcat
     4561 tttcagatgc taacattcta acagctttta caagagctgg ccaaaaagta agtattgttg
     4621 ttattcccca taaggcatgg attaaaaaca caacaatata tggaggataa agtgttaata
     4681 caagaccaga tattcctgta ataattaaag aacctgaaag caattttctg gctgaaatac
     4741 ggtctgctaa aaaaccacca aaaaggtatg ataccattgc aagtagacca taaatacttc
     4801 ctaagcttcc catttgtgta tttgtaagat taaatgcttg tgcaaaagta tcataataat
     4861 aattctttaa atatggtaaa gcatatataa atgctcctgc tacaaagagt attatcaaga
     4921 ctttgaaatt tttccaaatg cttggtttat ttacttcaac tgacatactt cctccttatg
     4981 taaatacctg taaattttca taataattaa ttattgctta ttttattgtt tattcaatat
     5041 ttaggaatat ttattaatat attaggttaa tctatcaata atttatggtg agttatgagc
     5101 taaaagtaaa ctcttttagc tcaccataat aattatattt ttatttatac taacatccag
     5161 agttattaga tatctttttt tcatagttct tccatagcat taaacctgtt attataacaa
     5221 aaagacctcc tcctatcatt tcaatctcat agattatatt atcttttaaa gtctccataa
     5281 ctggtgccgt tgagaaaagt atgccaacta cagttactac aagtaccatt ctggcaatat
     5341 tgatacaaac tgaatcactt tttgcaattg aatatggtct ttcttcattt ggtcttgtct
     5401 ttctaagtct aatataagat gtaaaaagca agacatatgg aaataatgct gttaatgctg
     5461 tcatagtaac aagaacattg tatatattat ctactgatgg taataagttt gttcctacaa
     5521 gaagtagact cactattata gcctgaacta ttactgcatt tgatggtata ttgtatttat
     5581 ttgtaacagt caattgctta ggaaatacac cttctcttac atttccaaaa agcatcttta
     5641 ctggacctgc tatataaagt attaatgctc ctagtactga taaagttatt ccaaatgcta
     5701 ctattcttat aaaccaactt cctataccta aatcttgtgc aactttagct aatgcatcta
     5761 gtatccctgt agatgctgca attttatctg gaggaagtat cattgttatt gctactgaac
     5821 ctaagatata gattcctcct attaaaacag ccacagttaa tatagcttta ggaaagtttt
     5881 tcttagcatt atccatttcg gttatgaaat tagcagtcgt ttcagctcct gataatgcaa
     5941 atattatagc agatatagaa actaatgaat tggcatctat ttttggtatt atattttgga
     6001 ttgtatatat cgatgctgat ggagcttttt tgagaataac tacagacata aatgccatca
     6061 ctataagtaa tatagcagga attgtagaac ctaatgctcc tgtattcgta aaaaattttc
     6121 caaatgccat cccacgagta cttactaagg acaacagcca aaatattatg agtgaaagtg
     6181 ctaaaataaa catcttattg tttgataaat ctggatttcc aagcatatat gcaatattta
     6241 tcgctaaaaa agtcaagaaa gaagaatacc aaaatatctt tgatgtccaa tttagccatg
     6301 acaccataaa tccccacttc tctccataag cttgtttaac ccattctcca agtccaccat
     6361 cttttggata tgttgcagcc aactctgcac atataagtga tgcaggaaca aagaatataa
     6421 aagcaaacaa taaccaaact ggtatagaac caagacccaa tccaaaacta gaggcagtag
     6481 atttagctat ccatctaatc ccaaatagtg ctgatatatt cataaatact aaatcccaaa
     6541 atccaatttt ctttttcact gtaattcctc caatttcaaa tttattcaga ctcatacaca
     6601 atgtattgac ttaaatatca agtataagtt cttcaacact tcttggataa ttagttataa
     6661 ttgtatcacc attcttacct actattgctg tatctgagtg tctaaatcct ccaactccag
     6721 gtatatatat ccctggctcc atagaaaata ccattccttc ttctaatatt aattcattgt
     6781 caaatcttaa atatggctct tcatgttctg ataaacctaa accatgacca attctatgat
     6841 ttgaatataa ctctagacca tactttgcaa caacagctct tgctgcttca tccaccattc
     6901 ttgctggtat accagctttt attgtatcta atccagcttg ttgagcctct actgcaatct
     6961 taaacacttc tttttgtctt tctgttggtt tgcctataat aaaagttctt tcattttctg
     7021 ctctatagtt ttcataccaa acctgtctac tatgtattac tatatctcct ctttgaagta
     7081 ttctagtatt ggaatctaaa tgaggttgtg ctgttctatc aatccctgaa caagtccagt
     7141 ttgcaaatcc tatatatgta tcaggataat tttcagaaac atattttagt aaagcattat
     7201 cccctgcaac gtcaaactcc aattcactca tcccaactct aacattttct aaagaccctt
     7261 ttattcctat atcagatagg taacctgcta tcttcaaaaa ttctatttct tttgcatctt
     7321 ttacatatct catttcaaat attttacttc caacatcttt gatagcaaat gacttatcag
     7381 ttatatactc tgaaaagctt gcagatacta tatctttttc tattccaaca gttgttcctt
     7441 tagggtattt atttaaaatt atatctaagt aatgtttatg tgatattcca tgttctttca
     7501 tttctggtat ttcataatac acatatacat tatcaacttt tgcaacttct ttagcatgta
     7561 attcttctaa tccaggtact attaattcta ttttatcttg agttactact gttacaattg
     7621 gtctagaata agttattgct ttaaatccac taatataaaa ttgattgtct ggttcaaata
     7681 ttaaagcaat ttcaatgctt ttttcattca tgtactttct caatctttct agtctcatat
     7741 tatttgacat aatgcacctc tttcttaata ttcagataat ttattcaata caacaaggat
     7801 gaatatatta ttttaaaatt cttaatattt ttagtacttg tcgtatttgt atgtaaatt
//
LOCUS       DAELLV010000171         7850 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.171, whole genome shotgun sequence.
ACCESSION   DAELLV010000171 DAELLV010000000
VERSION     DAELLV010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7850
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.171"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(254..1408)
                     /locus_tag="KPD58_002798"
     CDS             complement(254..1408)
                     /locus_tag="KPD58_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0266532.1"
                     /translation="MINKAIKFKFIIFYFFSYIIFAFASTKFTPFLSKLGYSAFERGI
                     ILSSYAITNILFQLLFGVLADKYQTMKKIVLISLSVYGIASVIMFSTESATFIIYLLL
                     VALSGGLLNTCCSLYDTWIIGCGDNINNYLSFIKTFGSIGWAIGSMVASYLILRFSYF
                     GLSVAIGLIVVLLIINILLLPDIEKINKKINVTNKDIIELLKDKKYILLVCILFLLYS
                     MVVANNCTVIDKMIALNATNSQISLKWSLQSLLEIPTYLAGSYLLKKYSSLNLLKLSA
                     IMITVQFLLFALTNDSNIIIILCVFQVFSTPLILITSKRLIFEVSPKKLRSSSQLIAL
                     SIFMGGSFLIIPTVAGFLSINIGYNYTLMVLSLFGCLAYCLIVLLNYLQE"
     gene            complement(1411..1878)
                     /locus_tag="KPD58_002799"
     CDS             complement(1411..1878)
                     /locus_tag="KPD58_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG0266533.1"
                     /translation="MFFKRKKEAITINSYIDGKIINLGEIPDKVFSTKVMGDGFAMIA
                     KDNILKSPTKGVISFIAQTNHAIGIKLQNDVQILIHIGLDSATTKKENFKLEVKQGQE
                     VNMGEKLVEIDNKLLENTLYIPLVIVENPNTVKYHLSNLNQEGNSGIKVINFD"
     gene            complement(1892..2380)
                     /locus_tag="KPD58_002800"
     CDS             complement(1892..2380)
                     /locus_tag="KPD58_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266534.1"
                     /translation="MITNKIKNITAVLKDENIFLSVVMVCFLILPVLYIVYLELTSLF
                     TNISFSQLLNNDVNASLNLISNFSAVYSAYVIYNIKKNKEIKENMIAFYLVLLGQIFQ
                     IYTITIVIMFTYIYKFVGIKQIISSIKELQWGKSYKALFSSIAVCLISLFVMIIRIKL
                     IM"
     gene            complement(2394..3725)
                     /locus_tag="KPD58_002801"
     CDS             complement(2394..3725)
                     /locus_tag="KPD58_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBG0266535.1"
                     /translation="MERKRQRILIAGGGSTYTAGIVTMLIESVAKFPIESIKLYDNND
                     ERQRKVAEACAIIVREKNPEIKFSYTTNPEEAFTDIDFVMAQIRVGLYALREQDEKIP
                     LKYGVVGQETCGAGGIAYGLRTIGPIIEMIDYMEKYSPNAWMLNYSNPAAIVAEACRV
                     LRPNSRIINICDMPVCLEEIFARVLGLNSRKDFDVRYYGLNHFGWWTSIKDKEGNDLM
                     PKLQEYCAKKGYEEFTPQGQHKESSWLETMRAAKDLLEIEPTTLPNTYLKYYLMADET
                     VEHANPNYTRANEIMDRREKDTFEECERIIKNGTARDTWFDASEHSQFIVELACALAF
                     NTQERFLLIVPNNGAIENFADDAMVEIPCLVGKDMVEPMSIGKIPTFQKGLMEQQVAS
                     EKLAVEAWIEKSYQKLWQSFTMSKTVPSAKVAKEILDEMIVANKDFWPTFK"
     gene            complement(3739..5304)
                     /locus_tag="KPD58_002802"
     CDS             complement(3739..5304)
                     /locus_tag="KPD58_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003233606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG0266536.1"
                     /translation="MMKKIQRFGGAMFTPTLLFAFAGIMVGFSIVFQNQSIMGSPATP
                     ENIWYQFWGVISSGAWMVFNQLPLLFAISLPIALAKKQQARACMEALATYLTFNYFVG
                     SMLSFWGKSFGVDFAAEISAGSGLVSIAGIKTLDTGMVGALLISGIVIYIHNRFYDKE
                     LPDFIGLFRGSSLVVAICFFVMIPVALLTCFIWPHIQNVIRHLQTFFINSGNIGVWCY
                     AFLQKILIPTGLHHFVYAPICYDSVVVPGGTSVYWATHIQDFQTSAKTLKEMYPIGFS
                     LSGLSKVFGSLGVFGAFYVTAKPEKKKKVLGLMIPATLTAVLTGITEPLEFTFLFVAP
                     LLFLVHAFLDACLQTISFALGVVGDFGGGIINWVVLNWLPLGMYHWKVYVVQVVVGII
                     FSFIWFFVFTFLIKKFDMKTPGREEDSEETKLYTKNEYLETKDEKGNKLSKASQQAYE
                     YIKLVGGAENVVDVTNCATRLRLTLKDDSIISKEEDFKAVGAHGLVHNGKAIQIIIGL
                     SVPSVREEFENLL"
     gene            complement(5474..6259)
                     /locus_tag="KPD58_002803"
     CDS             complement(5474..6259)
                     /locus_tag="KPD58_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG0266537.1"
                     /translation="MRLEEMIFLNYNKLNDTDLLIWKYITANKKHCSKMSINELAMKC
                     NVSRATITRFVKKLDLKGFSEFKVLLSWESEKVHVIENQSFDIACNSIIKYVEDQKNK
                     DYDALCKLLYESRTIYAYGSGDIQNVVAKQIKRMFLSCQEIIYDFEGRTFDDSFYNIV
                     SKNDVMIIISLSGENENTLNIARRLKLIGTKIISITEFKNNTLTDLSDESIYISATNI
                     SFLQMHPSYKLTMLYFILIELLFIKYSIYKRHRMITEGIDCKL"
     gene            complement(6426..7625)
                     /locus_tag="KPD58_002804"
     CDS             complement(6426..7625)
                     /locus_tag="KPD58_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HBG0266538.1"
                     /translation="MKKKVLFLVIILAFIILLGNSTYSQKNQLSEEEKVDDFNYVYNV
                     VKTGYPYLGVNKRLNNIDWLANKDEYTKRIKNTENDEEFIEELSSILSDLNNKHTEVI
                     DNKKRYELFKKSYSNNDWYDFLNDKKVVDRYDSMNLKIKMPDDIFVKKELILKDVIKG
                     QIGYMYLPSMSSKNGSINNDLKIIGDYINTLEKHKALIIDIRGNLGGSDRYWQGIVSK
                     LIKNDVKINGYRIYRNNNEIVKKYTNARNIKLNPVENLPASVKENAPKEIIKDFNDFE
                     DTSYTIKSTDDSKFKGNIYLLVDRKIYSSSESFAMFCKENKFATLVGETTGGDGGCID
                     PVLFNLKNSGLIVRMASCMYLNKNGICDEEFKTIPEFKVKDCKRTADFKDDNCIRKAL
                     ELENINY"
BASE COUNT         2783 a         1343 c          795 g         2929 t
ORIGIN      
        1 aatccttaag taaatattac attttaactg ttagcattac acttttgtaa catatggaag
       61 actattataa ttatagtaga aatatcttaa aataaaaact tgcttcactt ttttgtataa
      121 acaaagaaaa gaagctccta agaagcctct aatctataca ataattcttc tctataaaaa
      181 accaaaatac acattgattg tggcaaatac agatactata aacaagtgat attactacta
      241 tatattaaaa caattattcc tgaagataat tcaataagac tatcaagcaa taagctaagc
      301 atccaaaaag acttaatacc attaatgtat aattataccc aatattaatg ctcaaaaaac
      361 cagcaacagt tggaataatc agaaatgaac ctcccataaa tatacttaaa gctattaatt
      421 ggctactact tctaagtttt ttaggagaaa cttcaaatat taatcttttt gaagtaatta
      481 aaattaaagg agtagaaaac acttgaaaaa cacacagtat aataatgata tttgaatcat
      541 ttgttaaagc aaataataga aactgaactg ttatcataat agcacttaat tttaataagt
      601 ttaaactgct atattttttt aataaataag aaccagccag atatgttggt atctctaaca
      661 atgactgtaa agaccacttt aaagatatct gagaatttgt agcatttaaa gctatcattt
      721 tatctattac agtacagtta tttgctacca ccattgagta taataagaat aaaatacata
      781 ctagtaagat atatttttta tcttttaata attcaataat atctttatta gttacattta
      841 ttttcttatt aatcttttca atatcaggta ataatagtat atttataata agtaatacta
      901 caattaaacc aatcgctaca gataatccaa aatatgagaa tctaagaatc aagtaagatg
      961 ctaccatact tccaatagcc catccaatcg acccaaaagt cttaataaat gacaaataat
     1021 tattaatatt atctccacaa cctattatcc atgtatcata taaactacag caagtattta
     1081 ataatcctcc tgataaagca acaagtaata agtatattat aaaagttgca gattcagtag
     1141 aaaacataat aactgatgca attccataaa ctgataaact aattaaaaca atttttttca
     1201 ttgtttgata cttatcagct aatactccaa ataataattg aaacaatata ttggttattg
     1261 catagctcga taatataatt cctctttcaa acgctgagta acctaatttt gataagaaag
     1321 gtgtgaattt tgttgaagca aaggcaaaaa taatatatga gaaaaaatag aatatgataa
     1381 atttaaattt aatcgcttta ttaatcataa tcaatcaaaa ttgataactt taataccact
     1441 atttccttcc tgatttaagt ttgaaagatg atattttacc gtattaggat tctctacaat
     1501 aactaaagga atatataaag tattttcaag taacttatta tcaatttcta ctaacttttc
     1561 ccccatatta acttcttgac cttgtttaac ttctaattta aagttttctt tttttgttgt
     1621 tgcactatcc aatccaatat gaataagaat ttgaacatca ttttgcaatt taattcctat
     1681 agcatggtta gtttgagcaa taaaacttat tactccttta gtcggtgatt taagaatatt
     1741 gtcctttgct atcatagcaa atccatctcc cattacctta gttgaaaata ctttatcagg
     1801 aatttctcca agattaatta tttttccatc aatgtatgaa ttaattgtaa ttgcttcttt
     1861 ttttctttta aagaacatta tagacactcc tctacataat taactttatt ctaattatca
     1921 ttacaaataa cgatattaag catactgcaa ttgaggaaaa aagagcctta tagctctttc
     1981 cccattgtag ttctttaata ctgcttatga tttgtttaat tccaacaaat ttataaatat
     2041 aggtaaacat gataacaata gttattgtgt aaatttggaa tatctgccct aataagacta
     2101 aatagaaagc aatcatgttt tcttttatct ctttattctt ttttatattg taaataacat
     2161 atgcactgta tactgcacta aagttagaga ttaaatttaa acttgcatta acatcattgt
     2221 ttaaaagttg tgaaaatgaa atatttgtaa ataatgatgt caattctaaa tacactatat
     2281 ataaaacagg taatattaga aaacaaacca taaccactga aagaaatata ttttcatctt
     2341 ttaatactgc tgtgatattt tttattttat ttgttatcat gtttttgttc tccttattta
     2401 aaagttggcc agaaatcctt attagccaca atcatttcat ctaagatttc ttttgctact
     2461 tttgcacttg gaacagtttt actcatagta aacgactgcc ataatttttg ataacttttt
     2521 tctatccatg cttctacagc taatttttca gaagcaactt gttgttccat taaacctttt
     2581 tggaaagttg gaattttacc tatagacata ggttcaacca tatcttttcc aacaaggcaa
     2641 ggaatttcaa ccattgcatc atctgcaaaa ttttcaatag ctccattatt aggaacaatc
     2701 agtaaaaatc tttcctgagt attaaatgct aatgcacaag ctaattcaac aataaattga
     2761 gaatgctcac ttgcatcaaa ccatgtatcc cttgctgtgc cattttttat aattctttca
     2821 cattcttcga atgtatcctt ttctcttcta tccataattt catttgctct tgtataattt
     2881 ggattagcat gttcaacagt ttcatcagcc attaaataat attttaaata agtattaggt
     2941 aatgtagtcg gttctatttc caataaatcc tttgctgctc tcatagtttc taaccaacta
     3001 gattctttgt gttgaccttg aggtgtaaat tcctcatatc cttttttagc acaatattct
     3061 tgtaatttag gcattaaatc atttccttct ttgtccttaa tacttgtcca ccacccgaaa
     3121 tgattcaatc cataatatct aacatcaaag tctttacgag aatttaaacc taaaactcta
     3181 gcaaatattt cctctaaaca tactggcata tcacaaatat taataattct tgaatttggt
     3241 cttaatacac gacatgcttc tgcaactata gctgctggat ttgaataatt aagcatccaa
     3301 gcatttggag aatatttttc catataatca atcatttcga ttattggacc tattgttctt
     3361 aatccatagg caattccacc tgccccacaa gtttcttgac caacaacccc atattttagt
     3421 ggaatttttt catcttgttc acgtaaagca tataaaccaa cacgaatttg tgccattaca
     3481 aaatcaatat cagtaaatgc ttcttctgga tttgttgtat aactaaattt aatttctgga
     3541 tttttctctc taacaattat tgcacatgct tcagcaactt ttctttgtct ctcatcatta
     3601 ttatcatata atttaattga ttcaatagga aatttagcaa cactttcaat taacattgtt
     3661 acaattccag cagtataagt actaccccca ccagcaatta gtatcctctg tctttttctt
     3721 tccatctttc cttctccttt ataataaatt ttcaaattct tctcttacac ttggtactga
     3781 aagcccaata ataatctgaa tagcttttcc attatgcact aaaccatgtg cacctactgc
     3841 cttaaaatct tcttctttag aaataattga gtcatctttc aaggttaatc tcaatctcgt
     3901 agcacagttt gtaacatcta caacattttc tgctccacca actaatttta tgtattcata
     3961 tgcttgttga gaggctttac tcaatttatt tcctttttca tctttcgttt ctaaatattc
     4021 atttttagta tatagttttg tttcttcact atcttcttct ctaccaggag ttttcatatc
     4081 aaacttctta attaagaaag taaatacaaa gaaccagata aatgaaaata taatccctac
     4141 aacaacttga acaacatata ccttccagtg atacattcca agaggaagcc aatttaatac
     4201 aacccagtta ataattcctc caccaaaatc accaacaact cctaaggcaa acgaaatcgt
     4261 ttgcaagcat gcatctaaaa atgcatgtac taggaataaa agtggtgcta caaataaaaa
     4321 tgtaaattct aacggctcag taattccagt taatactgca gtaagagtag caggaatcat
     4381 taaacctaat acttttttct ttttttctgg ctttgcagtt acatagaaag caccaaatac
     4441 acctaatgaa ccaaacacct tcgataatcc ggataatgaa aatccgattg gatacatttc
     4501 ctttagagtc ttagctgaag tttgaaaatc ctgaatatga gtagcccaat atactgatgt
     4561 accacctgga acaacaacac tatcataaca aattggtgca tatacaaaat gatgtaatcc
     4621 agttggtatt aaaatctttt gtaagaatgc ataacaccaa actcctatat ttccactatt
     4681 tataaaaaac gtttgcaaat gtctaataac attttgaata tgaggccaaa taaaacaagt
     4741 taataatgca actggtatca taacaaagaa acaaattgca actacaagtg atgagcctct
     4801 aaataatcca ataaaatctg gcagttcttt atcataaaat ctattatgaa tataaatgac
     4861 aatacctgat attaacaaag caccaaccat accagtatct aatgttttaa taccagcaat
     4921 gcttacaagt ccacttccag cacttatttc agctgcgaaa tcaactccaa agctttttcc
     4981 ccaaaaactt aacatagaac ctacaaaata attaaaagtc aaatacgttg ctaaagcctc
     5041 catacaagct cttgcttgtt gcttttttgc aagtgctatt ggtaaagaaa tagcaaataa
     5101 taatggcaat tgattaaata ccatccaagc tccactagat attacacccc aaaattggta
     5161 ccaaatattt tctggtgtag caggtgaacc cataattgac tgattttgaa atacaattga
     5221 gaatccaacc attatcccag caaaagcaaa taatagtgtt ggtgtaaaca ttgcccctcc
     5281 aaaacgctgt attttcttca tcatataaat tactcccctc ctcatatttt tagtttatct
     5341 ttggccttag atatctatta ttttaacagg ttaataaata tatacaatct ttagggtaca
     5401 ttcggagctt attcacagtc aaataagttt ttgcacattt ttatgttcaa attctcataa
     5461 aaaaaaagaa tgactacaat ttgcaatcaa tcccttctgt aatcattcta tgccttttat
     5521 aaatactata tttaataaat aataactcaa tcaatataaa atataacatt gtcaatttat
     5581 aactagggtg catttgtaaa aatgagatat tagtcgctga aatataaatt gattcatcac
     5641 ttaaatcagt caatgtatta tttttaaact ctgtaattga aattatttta gttcctatta
     5701 attttaatct tctagcaata ttaagtgtat tctcattctc accacttagg gatataataa
     5761 tcataacatc atttttactg acaatattgt aaaaagaatc atcaaatgtt cttccctcaa
     5821 aatcatatat tatttcttga catgataaga acattctctt tatctgtttt gcaaccacat
     5881 tttgaatatc acctgaacca taagcgtata ttgttcttga ttcataaaga agtttacaca
     5941 atgcatcata atctttattt ttttgatctt caacatactt aattattgaa ttacatgcaa
     6001 tatcaaatga ctgattttct atgacatgaa ccttttctga ttcccagctt aataatactt
     6061 taaactcact gaaacctttt aaatctaatt ttttaacaaa tcttgtaata gttgcacgag
     6121 acacattaca tttcatagca agttcattta ttgacatctt actacaatgt tttttatttg
     6181 cagttatata tttccatatt aataaatctg tatcattcaa tttattataa tttaaaaaaa
     6241 tcatttcttc taatctcata aactattacc ccctttaatt aaaaatcgtc ataaatatta
     6301 taccaatatt caacattaaa ttacagcgta ttcttatatt tactatttag cccataaaat
     6361 tgactataaa aaatactgct ataattgatg tttaaaaaaa tgtagtttta attttattct
     6421 tttatctaat aattaatatt ttcaagttct agtgcttttc ttatacaatt atcatcttta
     6481 aaatcagcag tccttttaca atcttttact ttaaattctg gaatagtttt aaattcctca
     6541 tcacatattc cattcttatt taaatacata caacttgcca ttctaactat tagaccacta
     6601 ttttttaaat taaacaatac agggtctata caccctccat ctccgccagt agtttcacca
     6661 actaaagtcg caaatttatt ttctttacaa aacattgcaa atgactctga tgatgaatat
     6721 atttttctat caacaagtaa gtatatatta cccttaaatt ttgaatcatc tgtagatttt
     6781 atagtatagg aagtatcttc aaaatcatta aaatctttta taatttcttt aggtgcattt
     6841 tcctttacac ttgctggtaa attttctaca ggatttaatt ttatatttct tgcatttgta
     6901 tattttttta ctatttcatt attatttcta taaattctat aaccatttat tttgacatca
     6961 ttttttatga gtttagatac gattccctgc caatacctat cacttccacc taaattacct
     7021 cttatatcaa taattaaagc tttatgtttt tctaaagtgt taatataatc tccaattatt
     7081 tttaaatcat tatttataga gccattcttt gatgacattg atggtaaata catatatcca
     7141 atctgaccct taataacatc ctttaaaatt aattcttttt ttacaaaaat atcatctggc
     7201 atttttattt taagattcat agagtcatat ctatccacaa cttttttatc attaagaaaa
     7261 tcataccaat cattattaga gtatgatttc ttaaaaagtt catatctttt cttattatct
     7321 attacttctg tgtgtttatt atttaaatca gataatatag atgacagttc ttctataaat
     7381 tcttcatcat tttcagtgtt ttttattcgt tttgtatatt catccttatt tgctaaccaa
     7441 tcaatattat taagcctctt atttacacct agatatggat atcctgtctt tacaacatta
     7501 tacacataat taaagtcatc caccttttcc tcttcagaca actgattctt ttgagaataa
     7561 gtagaattac ctaataatat tataaatgct aaaataatta ctaaaaataa tactttcttt
     7621 ttcatgtaat tctcatccac tttcagattt tatattttaa tacattgtat cattatttta
     7681 ttatcaccta aacagttaaa agaaattctt gaagatatat tccactatta ttctatttaa
     7741 aaatttttat ttgtattttc aaaattattt catgaaagtt ataaaataac aattatattc
     7801 aaatatctat tttactgcta tatcctgaaa gtaaattctt atcagtgata
//
LOCUS       DAELLV010000172         7833 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.172, whole genome shotgun sequence.
ACCESSION   DAELLV010000172 DAELLV010000000
VERSION     DAELLV010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7833
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.172"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            103..1650
                     /locus_tag="KPD58_002805"
     CDS             103..1650
                     /locus_tag="KPD58_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG0266539.1"
                     /translation="MNKNSMTRIYSLNKIIFIIIGILGIVLLTRHIEVDIKVLILILL
                     TLFSINVFISYLKIKLYEDKINLGLINNSDKFINITKDENFLKHVQNYIISNEKENCV
                     MACFDLCRLKLINDIYGYELGDEVLNNILSNLKNYFGKEAIYGKLKSDVFVLIIKLEN
                     REQKIPYLVNLIKNKIVILSQIDSFKMDINIEASIGIYKFNNNEIDIKKAIDNADMAR
                     LKSKGLKHIEYVEFDNAMEEEIQSIMKIERDLFLAIKNKEFVIHYQPKVDSSTGNIIG
                     SEALIRWLHPSLGMVGPNKFIPIAEKNGLINNIGRWLIQEVFITINKWISEGINVLPV
                     SINLSRVELYKNDLVDFFKLMFNTYNIPKELIELEITETTALRDVKFISERLYEIKSL
                     GMNISLDDFGVGNSNFINLKGIPLDIIKIDRSLILDIVTNTKTKFMVKAIVNLSHDLN
                     VTVICEGVEDMHQVKVLSELGCNFIQGYVFFKPLDEMNYKKLLTDGSYINLKDTLLDK
                     CMTVEEE"
     gene            1653..2051
                     /locus_tag="KPD58_002806"
     CDS             1653..2051
                     /locus_tag="KPD58_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBG0266540.1"
                     /translation="MISEDVDYSIRIIRAIFNNGEDKPIDAKMILKSEDIPILTLYRL
                     LRKLCANNILTFNKEDGKTYMLNVSLDTISLRTIVEVIDGKIYINKCTRYVSNCSNCL
                     KGCKVNRELLEIEKEIYKLLSRKSIRQILE"
     gene            2091..3824
                     /locus_tag="KPD58_002807"
     CDS             2091..3824
                     /locus_tag="KPD58_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HBG0266541.1"
                     /translation="MDDISQDNFLLSKEYENSLDVDTKKASGIYYTPKIIVDYIVKKT
                     LKNHDIIKNPYPRILDISCGCGNFLLEVYDILYDLFEENIYELKKKYDENYWTVDNIH
                     RHILNYCIYGADIDEKAISILKDSLTNKKVVNDLDESDIKINLFCCDSLKKKWRYKFD
                     YIVGNPPYIGHKKLEKKYKKFLLEKYSEVYKDKADLYFCFYKKIIDILKQGGIGSVIT
                     PRYFLESLSGKDLREYIKSNVNVQEIVDFLGANIFKNIGVSSCILTFDKKKTKETYID
                     VFKIKNEDICINKFETLEELLKSSKFEHFNINQRLLSDEWILVNKDDETFYNKIQEKC
                     KYSLEDIAISFQGIITGCDKAFILSKDDVKLNLVDDKFLKCWIKSKNINKYIVDKSEY
                     RLIYSNDIDNENTNKRILDEIIGLYKTKLENRRECKSGIRKWYELQWGREKLFFERKK
                     IMYPYKSNENRFAIDYDNNFSSADVYSFFIKEEYLDKFSYEYLVGILNSSVYDKYFKI
                     TAKKMSKNIYDYYPNKVMKIRIFRDNNYEEIENLSKQIISILLNKSIDKGKVEKLQIK
                     MDNLIMDSLGI"
     gene            4056..5267
                     /locus_tag="KPD58_002808"
     CDS             4056..5267
                     /locus_tag="KPD58_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBG0266542.1"
                     /translation="MKGLVLEGGGTKGAYQIGAYKALRELGIEFQGVTGTSIGALNGA
                     YIVQNDIEVMEDIWLNYDYTHFMNIDRETYERFKNVDFTTKNLNAVFELINKARKNEG
                     IDISPLRQLMETTLNEDIIRKSKKDFGLVTVWWDKKINPYPLYIEDIPKGRLIDYLIA
                     SSSLPIFQTNMMDDKLYLDGMFCDNMPIGLLEDKGYEDVVVIRLIDDFIGKMNLNRHQ
                     NINIKTIIPSEYLGGSLNKDRDNVEKNIRLGYLDTMKAYERCTGIKYYFDVEYKYNEE
                     YCFDKFRSMSNETIQYICNLLNIKRDASLRTVMESVIPKLGEVLGLPKDFSYKDLFYC
                     IYEKKLEENNINRIKLYDFNKVIDVVNTNIDSNRNLASNFEVRPKISIKKNKNTKLLT
                     NCIIKDIKNQS"
     gene            5460..5720
                     /locus_tag="KPD58_002809"
     CDS             5460..5720
                     /locus_tag="KPD58_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HBG0266543.1"
                     /translation="MEKVVSIKNASGLHARPAGMFVKKAAEFKSTVEVVAKDKTVNAK
                     SIMGIMSLGLAQGEELKLVVNGEDEEVALAAMVELVESGFGE"
     gene            6074..7786
                     /gene="ptsP"
                     /locus_tag="KPD58_002810"
     CDS             6074..7786
                     /gene="ptsP"
                     /locus_tag="KPD58_002810"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBG0266544.1"
                     /translation="MAYKGIGASPGVALGKALVVEHSELVIEKKSIDNVEAEIAKLED
                     AVAVSKEELVKVKEKASEELGAEEAEIFEAHLLVLEDPELIGSAIDKIKTESVNAEYA
                     LNEIKEMFVSMFESMDNEYMKERAADIKDVTNRILRHILGIKVVDLSALSEEVVLIAH
                     DLTPSDTATMNKKMVLGFLTDIGGRTSHTAIMSRTLEIAAIVGLNDITSKVKDGDFVV
                     FNGDTGEVIVNPDEETINKYTELKAKYEDERKALQLLKGKPSVTLDGKHVELAGNIGT
                     PNDIEGLIKNDAEGVGLYRTEFLYMDRDSFPTEEIQYEAYKAVLEGMDGKPIVIRTLD
                     IGGDKELSYLSMEPEMNPFLGYRAIRLCLDRKDIFKTQLRALYRASVHGRLRIMFPMI
                     SSLEELLQAKEVVKEVLAELDSEGVAYAKDVEIGMMIEVPSAAVISDVLAKHVDFFSI
                     GTNDLIQYTCAVDRMNQKISYLYNQFNPAVLRLIKTVIDNAHKEGKWAGMCGESAGDQ
                     KMIPILLGMGLDEFSMSPISILPARKLITSVKESDMKKLADDVFNMGTAEEIKSYIEK
                     TFNI"
BASE COUNT         3246 a          689 c         1365 g         2533 t
ORIGIN      
        1 tatatgtgat tttaacttgc attgatttgc tatcaattgt gctttataat tagtatatat
       61 aaagtttaac tgtaagataa tttaaaaaat aggggaattt atatgaataa aaatagcatg
      121 acaagaattt acagtttaaa taaaataatt tttattataa ttggaatttt aggaatagtt
      181 ttactcacaa gacatattga ggtagatatt aaagttttaa ttttaatttt attaaccttg
      241 tttagtataa atgtatttat ttcatatctt aaaattaagt tgtatgagga taaaattaat
      301 ttaggattga taaataattc agataagttt ataaatatta ctaaagatga gaacttccta
      361 aaacatgttc aaaattatat tatcagtaat gaaaaagaaa actgtgtaat ggcttgtttt
      421 gatttatgta gactcaaact tataaatgat atatatggtt atgagctagg ggatgaagtg
      481 ttaaataata ttttaagtaa cttaaaaaat tattttggaa aagaagctat ttatggaaag
      541 ttaaaaagtg atgtttttgt attaataata aagcttgaaa atagggaaca gaagattcct
      601 tatttagtaa atcttattaa gaataaaatt gtgatactaa gtcagattga cagttttaaa
      661 atggatataa atattgaggc ttctattgga atttataaat ttaacaataa tgaaattgat
      721 ataaaaaaag ctatagataa tgctgatatg gcaagactta aatcaaaagg actaaagcac
      781 atagagtatg tagaatttga taatgctatg gaagaagaaa tacaatcaat tatgaaaata
      841 gagagagatt tattcttggc tattaaaaat aaagagtttg taattcacta tcaacctaaa
      901 gttgattcaa gtacaggtaa cattataggt tcagaggcat tgataagatg gttgcaccca
      961 tcattaggca tggttggacc aaataagttt atacctatag ctgaaaaaaa tggtctgata
     1021 aacaatattg gtagatggct aattcaagaa gtttttatta ccattaacaa gtggatttca
     1081 gaaggtataa atgtcttacc agtctcaata aatctttcta gagtagaact atataaaaat
     1141 gatttagttg atttttttaa actgatgttt aatacatata atattccaaa agaacttata
     1201 gaattagaaa ttacagaaac gacagcacta agagatgtaa aatttataag tgaaaggctt
     1261 tatgagatta aatctttagg tatgaatata tcattagatg attttggagt aggtaattca
     1321 aattttatca accttaaggg gataccacta gatattataa aaattgatag gtcacttata
     1381 ttggatatag ttacaaatac aaaaacaaag tttatggtga aagcaattgt taacctttca
     1441 catgatttaa acgtaactgt tatatgtgaa ggggtagagg atatgcatca ggtcaaagtc
     1501 ttaagtgaac tgggatgtaa tttcatacaa ggttatgtat tttttaaacc tttggatgag
     1561 atgaattata aaaaacttct tactgacgga agttatataa atctgaaaga tactctttta
     1621 gataaatgca tgacagttga ggaggagtaa ggatgatttc agaagatgta gattattcaa
     1681 taagaatcat aagagctata tttaataatg gagaagataa accaatagat gctaaaatga
     1741 tattaaaaag tgaagatata ccaatactaa cactatacag actgcttaga aagctttgtg
     1801 caaacaatat tcttactttt aacaaagaag atggcaagac atacatgtta aatgtgtcat
     1861 tggatacaat aagtctaaga actattgtag aagtaattga tggaaaaata tatataaata
     1921 aatgtacgag atatgtatct aattgcagta attgcttaaa aggttgtaaa gtaaatcgtg
     1981 aattgctaga aatagaaaaa gaaatttata aacttttatc gagaaaatct ataagacaaa
     2041 tactagaata aaaaattttc agctatgata ataattggag gttttcggtt atggatgata
     2101 tatctcaaga caattttcta ctgtctaaag agtatgagaa tagcttagat gtagatacta
     2161 aaaaagcaag tggaatatat tatactccta aaataattgt agattatatt gtaaaaaaaa
     2221 ctttaaaaaa tcacgatata attaaaaatc catatccacg aatacttgat atttcctgtg
     2281 ggtgtggaaa ttttctttta gaagtatatg atattttata tgacttattt gaggaaaata
     2341 tatatgaatt gaaaaagaag tatgatgaaa attattggac tgttgataac atacaccgtc
     2401 atatattaaa ctattgtata tatggtgctg atattgatga aaaagctata agtatattaa
     2461 aagatagctt aacaaataaa aaggtagtaa atgatttaga tgaatctgat ataaagatta
     2521 atctattttg ttgtgatagt cttaaaaaga aatggcgata taagtttgat tacatagtgg
     2581 gtaatccacc ctatattggt cataaaaaac ttgaaaagaa atataaaaaa tttctcctag
     2641 aaaaatatag tgaagtttat aaggataaag ctgacttata tttttgtttt tataaaaaga
     2701 ttatagatat tttaaagcaa ggagggattg gcagtgttat aactccaaga tattttttag
     2761 aaagtctatc aggtaaagat ttaagagaat atattaaatc aaatgtcaat gttcaagaaa
     2821 tagtggattt tttaggtgca aatatattta aaaatatagg ggtatctagt tgtattctaa
     2881 cttttgataa gaaaaaaaca aaagagacat atatagatgt atttaaaatc aaaaatgagg
     2941 atatatgtat aaataaattt gaaacattgg aagaacttct aaagagtagc aaatttgaac
     3001 attttaatat aaatcagaga ttgctatctg acgaatggat acttgtaaat aaagatgatg
     3061 aaacttttta taataagata caagaaaaat gtaaatatag cttggaagat atagcaataa
     3121 gttttcaagg aataattaca gggtgtgata aagcgtttat tttaagtaaa gatgatgtaa
     3181 agttgaacct tgtagatgat aaatttttaa aatgttggat aaaaagtaag aatataaata
     3241 aatatatagt agataaaagt gaatataggc taatatactc aaatgatata gataatgaga
     3301 atacaaataa aaggatacta gatgaaatta tagggctata taaaacaaaa ttagaaaaca
     3361 gaagagaatg taaaagtgga ataaggaaat ggtatgaact tcaatggggt agagaaaagc
     3421 ttttctttga aagaaaaaag ataatgtatc cttataaatc aaatgaaaat agatttgcta
     3481 tagattatga taataatttt tcaagtgcag atgtatattc tttttttata aaagaagagt
     3541 atttagataa attttcatat gaatatctag ttggaatttt aaattccagt gtttatgata
     3601 aatactttaa aataactgct aaaaaaatga gtaaaaatat atatgattac tatcctaata
     3661 aggttatgaa gataaggatt tttagagata ataattatga agagatagag aatttatcaa
     3721 agcaaataat atcaatttta ttaaataagt ctatagataa aggaaaagta gaaaaattgc
     3781 agataaaaat ggataacttg ataatggatt ctttaggtat ataagcagtt gtaagtatta
     3841 aaaaattata atcttttaat tgtttattta aatttagaaa taaaaagtta gccatataaa
     3901 aaatattaaa aatatatact tttcaatatt tttttgataa aataagaatg aatttgtgca
     3961 attttatttt tctccaataa ctaaaaatag ttttttgaat taatatttgc ttaagaataa
     4021 aaatcagaaa cgtttttgat atagtaggtg ataatatgaa aggattagtt ttagaaggtg
     4081 gaggaacaaa aggagcctac caaataggtg catataaagc tttaagagag cttggtatag
     4141 agtttcaagg agtaactggc acatcaattg gtgctttaaa tggagcatat atagttcaaa
     4201 atgatattga agtaatggaa gatatttggt taaattatga ctatactcat tttatgaata
     4261 ttgatagaga aacctatgag aggtttaaaa atgtagattt cactactaaa aatctcaatg
     4321 cagtgtttga gcttataaat aaagctagaa aaaatgaagg tatagatata agcccattaa
     4381 gacaactaat ggagacaaca ttaaatgaag acataataag gaaatcaaaa aaagactttg
     4441 gacttgttac ggtctggtgg gataaaaaaa ttaatccata tccattgtat atcgaagata
     4501 tacctaaagg aagattgatt gattatttaa ttgctagttc cagtttgcct atttttcaaa
     4561 cgaacatgat ggatgataag ttgtatcttg atggaatgtt ttgtgataac atgccaatag
     4621 gactcttgga agacaaaggt tatgaagatg ttgttgtaat taggttgatt gatgatttta
     4681 ttgggaaaat gaatctaaat cgacatcaaa atataaatat aaaaactata ataccatcag
     4741 aatatctagg aggttctctt aataaagata gagataatgt agaaaaaaac atccgactag
     4801 gttacttaga tactatgaaa gcatatgaaa gatgcactgg aattaagtat tattttgatg
     4861 tagagtataa atataatgaa gaatattgtt ttgataaatt tagaagtatg agtaatgaaa
     4921 caatacaata tatctgtaat ttattgaata taaaaagaga tgcttcactt agaaccgtta
     4981 tggaaagtgt aataccaaag cttggtgagg tgcttggatt gccaaaggat ttttcatata
     5041 aagatttgtt ttactgtata tatgaaaaga aacttgaaga aaataatata aacaggataa
     5101 aattatatga ttttaacaag gttatagatg ttgtaaatac aaatattgat tctaatagaa
     5161 atttagcttc taattttgaa gttagaccta aaattagtat taaaaagaac aaaaacacaa
     5221 aattattgac aaattgtatt ataaaagaca taaaaaatca aagttaaata aaaaacaaaa
     5281 aaaaatttgt aattatactc gaaaaaagtg tatattagta gtataatatt tttgtagcac
     5341 tagtgggaac aaaaaatgac cataggacaa aaaagtacca cttagtacca ttaaagtaaa
     5401 aaacaggaaa acaattttaa aaaatataaa atgtaaagtt tataggagga tttaaaaaaa
     5461 tggaaaaagt agttagtatc aaaaatgcaa gtggattaca tgctagacca gcaggaatgt
     5521 ttgttaagaa ggcagctgaa tttaaatcaa cagtagaggt tgtagcaaaa gacaaaactg
     5581 taaatgcaaa atcaataatg ggaataatga gtttaggttt agctcaagga gaagaattaa
     5641 agttagttgt aaatggagaa gatgaagaag tagctttagc tgcaatggtt gaattagttg
     5701 aaagtggatt tggagaatag atataataat tttttaaaaa tttgatattt agaatctaat
     5761 attacttaaa gtaaagtaac agagaagtat taaacatata cagaccaaca tttgtagata
     5821 tttaatttct ttgttacttt catgtttatt taatcagaag attaagacta ttgttaattg
     5881 ggtataaaaa tataaatatt ttagaaaatg aaataagata tttttaatca taaattcaga
     5941 gtaaaaatac agtaacaaat agtttataaa attttataaa aaattataag taaataaaac
     6001 tataaaaatg taaattattt atataaattt ataaagttta tgtgctagat gatatttttt
     6061 aggaggtagg gttatggctt acaaaggaat aggtgcttct cctggtgttg ctttagggaa
     6121 agcgttagta gttgaacata gtgaattagt aattgaaaaa aaatctatag ataatgttga
     6181 agctgaaatt gctaaattag aagatgcagt tgcagtttct aaagaagaat tagtaaaagt
     6241 taaggaaaag gcttctgaag aattaggagc agaggaagca gaaatatttg aagcacattt
     6301 attagtttta gaagacccag agttaattgg ttctgcaata gataaaatta aaactgaaag
     6361 tgtaaatgca gagtatgctt taaatgaaat aaaagagatg tttgttagca tgtttgagtc
     6421 tatggacaat gagtacatga aagaaagagc ggcagatata aaagatgtta caaatagaat
     6481 acttagacat atattaggaa ttaaagttgt tgatttatct gctttaagcg aagaagttgt
     6541 attaatagca catgatttaa ctccatcaga cacagcaact atgaataaaa aaatggtttt
     6601 aggattttta actgatatag gtggaagaac ttctcatact gctatcatgt ctagaacttt
     6661 agaaatagct gcaatcgttg gattaaatga tataacttca aaagttaagg atggagattt
     6721 tgtggttttc aatggagaca caggtgaagt aatcgtgaat cctgatgaag agactataaa
     6781 taaatatact gaattaaaag ctaagtatga agatgagaga aaagcattac agctattaaa
     6841 aggaaaacca tctgtaactt tagatggaaa acatgtggaa ttagctggta acataggaac
     6901 acctaacgat atagaaggtc taataaagaa tgatgctgaa ggtgttggtc tttatagaac
     6961 agaattttta tacatggata gagattcttt cccaacagaa gaaatacaat atgaagcata
     7021 caaagctgta ttagaaggaa tggatggaaa gcctatagtt ataagaacat tggatatagg
     7081 tggagataaa gaattaagtt atttaagtat ggaacctgaa atgaatccat tcttaggtta
     7141 cagagcaata agactttgcc tagatagaaa agatatattt aagactcaat tgagagcttt
     7201 atatagagct agtgttcatg gaagattaag aatcatgttc ccaatgattt catctttaga
     7261 agagttgtta caagctaaag aagttgtaaa agaagtttta gcagaacttg attctgaagg
     7321 tgtggcttat gctaaagatg tagaaattgg tatgatgata gaagttccat ctgctgctgt
     7381 gatatctgat gtattggcta aacacgtaga tttcttctct ataggaacta atgatttaat
     7441 tcaatacact tgtgcagttg atagaatgaa tcaaaagata agttatcttt acaatcaatt
     7501 taatccagct gttttaagac ttataaagac tgttatagac aatgcacata aagaaggaaa
     7561 atgggcaggt atgtgtggag aatctgctgg agaccaaaaa atgataccaa tacttttagg
     7621 tatgggatta gacgagttct ctatgtctcc aatatcaata cttcctgcta gaaagttaat
     7681 aacttcagtt aaagagtcag atatgaaaaa attagctgat gatgtattta atatgggtac
     7741 tgctgaagaa ataaaaagct atatagaaaa gacttttaat atataattaa tgttatatag
     7801 ttactaaaat tcatttttct aaataacaaa att
//
LOCUS       DAELLV010000173         7828 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.173, whole genome shotgun sequence.
ACCESSION   DAELLV010000173 DAELLV010000000
VERSION     DAELLV010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7828
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.173"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            400..1191
                     /locus_tag="KPD58_002811"
     CDS             400..1191
                     /locus_tag="KPD58_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HBG0266545.1"
                     /translation="MYKVDLNSDLGESFGTYKIGLDEEVLKYISSANIACGFHAGDPS
                     HMEKTVQLAKKNGVKIGAHPGFLDLIGFGRREMKITKQEAKDYTKYQLGALMAFASSN
                     GCNIQHVKPHGALYNMAAKDKELAMGICEAIYEVDKDIILLGLYNSEMINSAKEIGLR
                     FANEVFADRAYDNNGFLVPRNVEGAVIHDTKHAIDRVVRMVKEGTVETLTGEVIHIKA
                     DSICVHGDNPKAIEFVKEIRKRFELEGIEVCPLENIEVCSSENIV"
     gene            1220..2440
                     /locus_tag="KPD58_002812"
     CDS             1220..2440
                     /locus_tag="KPD58_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HBG0266546.1"
                     /translation="MSTKNITDKTKNKKDVGALIGAAFIMATSAIGPGFLTQTAQFTQ
                     DFGPNFSFVILITTILFIGAQVNVWRVIGVSGLRGQDIANKIIPGLGYLVAFLVALGG
                     LAFNIGNVGGAALGMNVMFNMNMTLGTVLSGLIAIFVFMSKNSNSLVDKITKFLALGM
                     IIIVGYVAISNHPPVGEAVSRMVKPENPKGLIFPIITLLGGSVGGYITFAGGHRLIDG
                     GITGEENIKEITKSSLLGILVATMMRVLLFLAILAVVSKGLQLDPENPAASAFKFSAG
                     AIGYKFFGLVLWSAAITSVIGAAYTSVSFLKTLNPFIDKYEKYFIIAFIAISTLIMAF
                     IGKPATLLILAGALNGLILPITLGIMLIASKRKDIVGDYKHPTWLLIFGLIVVLISAY
                     TGITSLSSLGALFA"
     gene            2460..3155
                     /gene="pxpB"
                     /locus_tag="KPD58_002813"
     CDS             2460..3155
                     /gene="pxpB"
                     /locus_tag="KPD58_002813"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HBG0266547.1"
                     /translation="METRYLISGDKAVVAEFGNEISEDINKKVISFMRAIEISNLKGI
                     VTEMVPTYRSLMISYNPLEIDFDSLIENLKKIEDNLESIVLPKPKIHEIPVCYDEVFG
                     IDIKNVASYNNLTVDEVIKIHTSREYLIYMLGFTPGFPYLGGMDERIATPRLEVPRTK
                     IYGGSVGIAGSQTGVYPIDSPGGWQIIGRTPLKLYDENREEQILLRAGDFIKFVPITL
                     DEFIEIEKNNLSI"
     gene            3185..4171
                     /locus_tag="KPD58_002814"
     CDS             3185..4171
                     /locus_tag="KPD58_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase"
                     /protein_id="HBG0266548.1"
                     /translation="MGFKVELGGFQTLIQDRGRVGYGQYGVSGCGAMDEYAHRVGNIL
                     VGNSEDEASLEVLMLGPTITFDEYTQIAVTGGDLGAKINGKEIQNWRSYQINPGDVLS
                     FRGVKSGARAYVSIAGGIDVPLAMGSKSTYTRAKIGGFEGRALKKGDYINTFIQEKDF
                     TINKKLSSKYIPTYSSEIVLRIVKGPQFDAFSNGEVEKFLSNKYKVTNEIDRMGCRLD
                     GESIKHLNGADIISDGISYGAIQVPGHGKPIIMLSDRQTSGGYTKIGNVISVDLYKLA
                     QAKPNDVVKFELVDIYEAHRLLREQEDKIQDIYKSMKNIRVVKAKVLNDIAV"
     gene            complement(4366..4932)
                     /locus_tag="KPD58_002815"
     CDS             complement(4366..4932)
                     /locus_tag="KPD58_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0266549.1"
                     /translation="MLGEKMRNIRKSKKKTLSDVSKLTDLSISYISQIERDAIEPSLS
                     SLRKIAEVLDTPLYMFMDDNNTDDLVIRKEDRVMMKFPKSEMFYEIVSPMPTTEFTPS
                     ILFVEFELKPESEDAKDYIYHASEEIMVVTSGVVDICMGEKVVRLNPGDSTFIKANVP
                     HKIINPSKDTIARGYGVISPPIWPIKSK"
     gene            5284..6351
                     /locus_tag="KPD58_002816"
     CDS             5284..6351
                     /locus_tag="KPD58_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HBG0266550.1"
                     /translation="MKITDIKFEKLRIKLKKPVVVSFGVIEYGESIILKIETDEGYCG
                     FGEAAPLAAVTGEVLDNVLSVLLMFKKELIGKDPLDIETIHTIMDGIIIGNTSAKAAI
                     DIALYDIKGKIMNAPLYKVLGGFDSKVQTDITISIDKPEKMAVEALERVKEGFRILKL
                     KAGINPEDDIEAVKLIREAVGESIRIRIDANQGWNVNSSINTIKKLEEFNVDAIEQAL
                     PHWDLDGTAYIRNKSNTKIMIDESLHSPIDAIKAIKKNAVDTFNIKLMKSSGIYPAIK
                     INNIAEASGVNCMLGCMLETRIGITAAANLIASKKNITEADLDSFMFCEELEGISGGF
                     VMDGDIMNLVNKPGLGIEVNL"
     gene            6370..7530
                     /locus_tag="KPD58_002817"
     CDS             6370..7530
                     /locus_tag="KPD58_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HBG0266551.1"
                     /translation="MNTLFSNPGSILAVMTSMIALGFYLQRYKAIKSLGPALTIIIMG
                     IILSNLKVVPVSTELYGTISTYAIPISMTIMLMSVDLKEMTKLSREPLIAIFVAVLTV
                     SIMAFLFGLVFAEKISEGWKVAGMFVGTYTGGSANLTAIGTGLNVSRQTLAAANAADY
                     VIGVPTLIFMFALPAILKNSKKFKKLWPYHVEESELEDCQNEEFMESKEWSIKDIAWM
                     LAIGFVVTEVSTILAGYFNSSFSSAARILLVTTISIIIAQLKPVKKLKGNLDLGLFVA
                     LFFLCTIGFSVDIKEFLGSTFTITLYCFSIIFASFVFHLGITRLLKIKYQYVILSIVG
                     AIADGPTSALVAASAKWNSLVSVAVVMGVIGGVLGNYAGISVAYAIKMSLGL"
     gene            7543..>7828
                     /locus_tag="KPD58_002818"
     CDS             7543..>7828
                     /locus_tag="KPD58_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBG0266552.1"
                     /translation="MIKFKLYISGLYSGNVIFDGDLLIEKLNPFTNKIESLKPISKEE
                     NTYYLNLTKIDLKSLFNNFDVYTKSLVNTDKDTVINNLGETHSKLNEYIWV"
BASE COUNT         3010 a          874 c         1436 g         2508 t
ORIGIN      
        1 aatataaaga tattttactt acaaagaatg ataattatat agatgaaaga aacaaatgaa
       61 tgaagaaaaa tgattctgtc ttgcatataa agtgattaaa aatgaaaaat actttaaaaa
      121 tgaaggttaa agcccttatt ttaagtgaaa ataattaatt attaactaaa aaatggtaaa
      181 aagatttttt gaaaaagaaa tacaaatgaa tgttcaatta aaataattgc aaaaatattc
      241 cttaaatggt aaaattatat taattaaatg caatagaata gttgcaaaaa taaaattgaa
      301 tacaagtgat aaaatatttc atgcaatata taaaataaac aaaatttaaa aattaaaact
      361 acttattcta tcaaaatgtt agcgaggagg ggtcataaat tgtataaagt agatttaaat
      421 agtgatttag gagaaagttt tggaacgtat aagattggtt tagatgaaga agttttaaaa
      481 tatatttcat cagccaatat agcttgtggg tttcatgcag gtgaccctag tcatatggaa
      541 aaaacagtac agctagctaa aaaaaatgga gtgaaaatag gcgcacatcc aggtttccta
      601 gatttaatag gatttggaag acgtgagatg aaaataacaa agcaggaagc taaagattat
      661 acaaaatatc aactgggtgc tttaatggca tttgcaagtt caaatggatg taatatacaa
      721 catgtaaaac cacatggagc attatacaat atggcagcta aagataaaga gttagctatg
      781 gggatttgtg aagctatata tgaagtcgat aaagatatta tactgttggg gctatataat
      841 agtgaaatga taaattctgc taaagaaatt ggacttaggt ttgcaaatga agtatttgca
      901 gatagagctt atgataataa tggttttttg gttccaagaa atgtagaagg agcagttata
      961 catgatacaa aacatgcaat agatagggtt gttaggatgg taaaagaagg aactgtagaa
     1021 actttaacag gtgaagttat acatatcaaa gcagattcta tatgtgtaca tggggacaat
     1081 ccaaaagcaa tagagtttgt caaagaaata agaaagcgtt ttgaattaga aggtatagaa
     1141 gtttgtccat tagaaaacat agaagtttgt tcgtcagaga acatagtata agactgataa
     1201 catgaggggg agataaaata tgagtacaaa aaatattaca gataaaacta aaaataaaaa
     1261 agatgtcgga gcacttatag gggctgcatt tataatggca acatcagcaa ttggaccagg
     1321 gtttttaact cagactgcac aatttaccca agattttgga ccgaattttt catttgtaat
     1381 tttaattaca acaatccttt ttataggggc tcaagtaaat gtatggagag taattggtgt
     1441 atcaggactt agaggtcagg atatagcaaa taaaataata cctggattag gctatttagt
     1501 agctttttta gtagctcttg gaggattagc ttttaatata ggaaatgttg gtggagctgc
     1561 acttggtatg aatgtaatgt ttaatatgaa tatgacatta ggaactgtat taagtggatt
     1621 aatagctata tttgtattta tgtcaaaaaa ttcaaattct ctagtcgata agataactaa
     1681 atttttagca ttaggaatga taattatagt tggatatgta gcaatatcaa atcatcctcc
     1741 tgttggagag gcagtatcaa gaatggtaaa accagaaaat ccaaaaggac tgatattccc
     1801 aataataact ttacttggag gaagtgttgg aggttatata acttttgctg gaggacatag
     1861 attaatagac ggtggaataa caggagaaga aaatataaaa gaaataacta aatcttctct
     1921 tttgggtata ttagttgcca caatgatgag agttctttta tttttagcaa tactggcagt
     1981 ggtttcaaaa ggtcttcaac ttgacccaga aaatccagca gcttcagctt ttaaatttag
     2041 tgcaggagca ataggatata aattctttgg acttgtatta tggtctgctg caattacatc
     2101 agttatagga gctgcttata catcagtttc tttcttaaag acactaaatc cttttataga
     2161 taaatatgaa aaatatttta taatagcatt tatagcaata tcaacactta taatggcatt
     2221 tataggaaaa ccagctactt tattaatttt agcaggagct ttaaatggat tgatacttcc
     2281 aatcacatta ggaattatgc ttatagcatc taaaagaaaa gatattgttg gagattacaa
     2341 gcatcctaca tggcttttaa tatttggatt aatagtagtt ttaatatctg catatacagg
     2401 aataacttct cttagcagcc taggtgcttt atttgcttaa tttagaagag gtgacattta
     2461 tggaaactag atacttgata tcaggggata aggcagtggt tgctgaattt ggaaatgaga
     2521 tatcagaaga tataaacaaa aaagtaattt catttatgag agcaatagaa atatcgaatc
     2581 ttaaaggtat tgtaacagaa atggtaccaa catataggtc acttatgata agttataatc
     2641 cattagaaat agattttgat agtttgattg agaatttaaa aaaaatagaa gataatttgg
     2701 aaagtatagt attaccaaaa cctaaaatac atgagattcc agtatgttat gatgaggttt
     2761 ttggtataga tattaaaaac gttgcaagct acaataatct cactgtagat gaagttataa
     2821 aaatacacac aagcagagag tacttgatat atatgctagg ttttactcct ggatttccat
     2881 atctaggtgg tatggatgaa agaatagcta cacctagatt agaagtgcca aggactaaaa
     2941 tttatggtgg gagtgttgga atagcaggtt cccagactgg tgtatatcct attgatagtc
     3001 ctggtggatg gcaaataatt ggtaggacac ctttaaaatt atatgatgaa aatagagaag
     3061 aacagatttt gcttagagct ggagatttta taaaatttgt tccaataaca ttagatgaat
     3121 ttatagaaat cgaaaaaaat aacttaagta tttaaatatc gttatagtac agggagtggt
     3181 atatatggga ttcaaagttg aattaggagg tttccaaaca ttaatacaag acagaggtag
     3241 agttggctat ggtcaatatg gtgtatctgg atgtggagca atggatgagt atgcacatag
     3301 agtaggaaat atattagtag gaaattctga agatgaggct tctttagaag ttttaatgtt
     3361 aggtcctaca attacctttg atgaatatac gcaaatagca gtaactgggg gagatttagg
     3421 ggctaaaata aatggaaaag aaattcaaaa ctggaggtca tatcaaataa atccaggtga
     3481 tgtattatca tttagaggtg tgaaatcagg tgctagagcc tatgtgtcaa tagctggtgg
     3541 aatagatgtt ccacttgcaa tgggaagtaa gtcaacttat acaagagcta aaattggtgg
     3601 atttgaaggt agagcactta aaaaaggaga ttatataaat acttttattc aggaaaaaga
     3661 ttttacaata aataaaaaat taagttcaaa atatattcca acttatagtt cagaaatcgt
     3721 actaagaatt gtaaaaggtc ctcaatttga tgctttttct aacggtgaag tagagaaatt
     3781 tttatcaaat aaatacaaag ttacgaatga aatagataga atgggttgta gactagatgg
     3841 agagagtata aagcacctta atggagcaga tattatatct gatggtattt cttatggagc
     3901 tatacaagta cctggtcatg gaaaaccgat tataatgtta tcagatagac agactagtgg
     3961 tggctatact aagataggga acgttataag tgtagatttg tataagttag cacaagcaaa
     4021 gccaaatgat gtagtaaaat ttgaacttgt agatatatat gaagcacata gactattgag
     4081 agagcaagaa gataagattc aagatatcta caagagtatg aaaaatataa gagttgtaaa
     4141 ggcaaaagta ttaaatgata tagcagttta attttaacaa atacaatttt tattataatt
     4201 tataatataa cttacaaata gctatttact atttaccata attaagatgg tttagattag
     4261 tcaaatagct atttttttag tttgagtaaa tattttaaat ttaaaataaa agcaatattt
     4321 aaatctatta tattatctac aaaaagtagg tgtgtcatat tgagtttatt tagattttat
     4381 tggccaaatt ggaggtgata taactccata tccacgagct attgtatctt tacttggatt
     4441 gatgatttta tgtggtacat tagcttttat aaatgttgag tcacctggat ttaatctaac
     4501 taccttttca cccatacaaa tatcaaccac accactcgtt accaccataa tttcttctga
     4561 tgcatgatat atataatctt tggcatcctc actttctggt ttaagttcaa actctacaaa
     4621 taaaattgaa ggtgtaaact cagttgtagg cattggagat actatctcgt aaaacatttc
     4681 actctttgga aatttcatca taactctatc ttcttttcta atgactaaat catcagtatt
     4741 attgtcatcc ataaacatat ataaaggagt gtctaaaact tcagcgattt ttctcaatga
     4801 agacaaggat ggttcaatag catctctttc tatttgagag atataactta tagataaatc
     4861 tgtcaatttg gaaacatcac ttaatgtttt tttcttgctt tttcttatat ttctcatctt
     4921 ttcacctagc ataagaacct ccaaataata aaattaatat aaaatattcg cttaaaacaa
     4981 agtaattata gcacaacata gaagtagtgg caataagaat acatagtaga ttttgacaat
     5041 taaaatttaa ttaaatccaa ataaaagtct atgaaaatga attattgtta aattaataca
     5101 aagaaaataa taataaaaaa atttaataga agagtattaa ttgaattaag attatcacat
     5161 tatattttgg aaaagagtaa attttcagta aattatcttg acatatatat tgaagtaata
     5221 atataattta aagtataaaa ttttaaaaat tgaattataa cttcaaaaaa taaggagggt
     5281 aatatgaaga taacagatat caaatttgaa aagcttagaa ttaaattaaa aaaacctgta
     5341 gtagtttcat ttggagtaat tgagtatgga gaaagtataa ttttaaaaat tgaaactgat
     5401 gaagggtatt gtggatttgg cgaagcagca cctcttgcag cagttacagg tgaagttttg
     5461 gataatgttc tttcagtatt gcttatgttt aaaaaagagt taataggcaa agacccacta
     5521 gatattgaaa ccatacatac catcatggat ggaattatta taggtaatac ttctgcaaaa
     5581 gcagctattg acatagcttt atatgatatt aaaggaaaaa ttatgaatgc acctctttat
     5641 aaagttcttg gaggatttga tagtaaagtc caaacggata ttactataag tatagataag
     5701 ccagaaaaaa tggcagtaga agctttagaa agagtaaaag aaggttttag gatattgaag
     5761 cttaaagcag gtataaatcc tgaagacgat attgaagctg taaagttaat tagagaagcc
     5821 gttggagaga gtataagaat tagaattgat gcaaatcaag gatggaacgt aaatagcagt
     5881 ataaatacta tcaaaaagtt agaagaattt aatgttgatg caattgaaca agcacttcca
     5941 cattgggatt tagatggtac tgcttacata agaaataaaa gtaacacaaa aattatgata
     6001 gatgaatcat tacattcacc aatagatgca attaaggcta taaaaaagaa tgcagttgat
     6061 acatttaata ttaagctgat gaaatcaagt ggaatatatc ctgctataaa aatcaacaac
     6121 atagctgagg caagtggagt aaattgtatg ttaggatgta tgctggaaac gagaattggt
     6181 ataacagcag cagcaaattt aatagcatct aaaaagaaca taacagaagc tgaccttgat
     6241 agttttatgt tttgtgaaga attagaaggt atatctggag gatttgtaat ggatggagat
     6301 attatgaatc ttgtcaataa accaggttta ggaatagaag taaacttatg ataaatttgg
     6361 aggttaaata tgaatacact tttttcaaat ccaggtagta tacttgctgt aatgacatca
     6421 atgattgcat tgggttttta cttacaaaga tacaaagcca ttaaaagctt aggaccagca
     6481 cttacaataa tcataatggg aataatactt tcaaacttaa aagtagttcc agtaagtact
     6541 gagttatatg gaactatttc aacatatgct attccaattt ctatgactat aatgttgatg
     6601 agtgttgact taaaagaaat gacaaaatta tcaagagaac ctttaattgc aatttttgta
     6661 gctgtattaa ctgtaagtat catggcattt ttatttggat tagtatttgc agaaaaaata
     6721 tcagaaggtt ggaaggttgc tggaatgttt gtaggtacat atactggtgg aagtgctaat
     6781 cttacagcta ttggaacagg tcttaatgta agtagacaaa ctcttgcagc agcaaatgca
     6841 gcagactatg taattggagt accaacattg atatttatgt ttgctctacc agcaatatta
     6901 aaaaattcaa agaagtttaa aaaactttgg ccatatcatg tagaagaatc agaattagaa
     6961 gattgtcaaa atgaagaatt tatggagtca aaagaatgga gtattaaaga tatagcttgg
     7021 atgttggcta taggttttgt tgttacagaa gtttcaacaa tacttgctgg atatttcaat
     7081 tcaagtttta gtagtgcagc aagaatactt ttagttacaa caatttcaat tataattgct
     7141 caattaaagc cagtaaagaa actaaaggga aatttagatt taggattatt tgttgcatta
     7201 ttctttttat gtacaatagg tttttcagta gatataaaag agtttttagg ttcaactttt
     7261 actataacac tttactgttt tagtataatt tttgcttcat ttgttttcca tttaggcata
     7321 actagacttt taaagataaa gtatcaatat gtaatacttt ctatagtagg agcaattgct
     7381 gatggtccta cttcagcttt agtagctgct tctgcaaagt ggaattcgct tgtaagtgta
     7441 gcagtcgtaa tgggagtaat tggtggagta ttaggaaatt atgcaggtat atcagtggca
     7501 tatgcaataa agatgtctct aggattatag gggggagttt aattgataaa atttaaatta
     7561 tacatatctg gactatacag tggaaatgta atctttgatg gagatttatt aattgaaaag
     7621 ttgaatcctt ttacaaataa aatagaaagt ttaaaaccaa taagcaaaga agaaaataca
     7681 tattatttaa atcttacaaa aatagattta aagtctttat ttaataattt tgatgtatat
     7741 actaaaagct tagtaaacac agataaagac acagttataa ataacttagg agaaacgcat
     7801 tctaaactta atgaatatat atgggttc
//
LOCUS       DAELLV010000174         7824 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.174, whole genome shotgun sequence.
ACCESSION   DAELLV010000174 DAELLV010000000
VERSION     DAELLV010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7824
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.174"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            28..957
                     /gene="dptG"
                     /locus_tag="KPD58_002819"
     CDS             28..957
                     /gene="dptG"
                     /locus_tag="KPD58_002819"
                     /inference="COORDINATES: protein motif:HMM:TIGR03236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA phosphorothioation-dependent restriction
                     protein DptG"
                     /protein_id="HBG0266553.1"
                     /translation="MELIILKNLPELENEETSKSIYKCICKNVSKLFQEDLEFMLNYP
                     ELIINEFERLLKYYYFFYVSQLSIKLSHFFDGDRDRTEKVYFILSWEKASKTRISYNM
                     GWKMLESHIRILFSHVHTLEILNTNNDISKYDYVDLNKIIKNLSENERLRLYENIIDI
                     KNTYVKSITDVQWDDYDEQTKVYKNDNIKEEICDLHKRIDYQFKKSSREANYKRYKNL
                     FEEYCKLNFLKKAGPLGNVLKINQEELLFMTKLCMKNKTKIKLKELFEEYEKRGIYFD
                     RDSQSKVVELFEKLNIIEKKSDSGDAQYVRSIL"
     gene            938..4909
                     /gene="dptH"
                     /locus_tag="KPD58_002820"
     CDS             938..4909
                     /gene="dptH"
                     /locus_tag="KPD58_002820"
                     /inference="COORDINATES: protein motif:HMM:TIGR03237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA phosphorothioation-dependent restriction
                     protein DptH"
                     /protein_id="HBG0266554.1"
                     /translation="MSDQFYRYLAKNLVKYFESREIKSGDKFNIQFEREEQVKIMYDV
                     LEKESNSRPFTYNIMNGSKYQTFSMNVSGIQLIVASNNNTTNDFLIRLRNLIGNVASD
                     EFKDSAILFIHNMTLDSLIRGTESLQKEGMPFHTKTLVKDIQNKLENDKLKEDIKHIL
                     YFVLDRMNKSILEDNSSIFQYENILDILNKGRIEKSEYSKFGLFYDDNMLDNKSSKAI
                     KERLLDNEKLFSIVNSIHQYGDPKLELEKHFDSKGIDLLKDENWKYSTYKDIKSSQER
                     MKNKKVELLCIECVENLKLWSKPDIENKSKRKNILLFNKDKLDIINLCIKFDKKIEKT
                     SFSIDKMSKTYTEVKASGNKAYISININISETTFTKVKYNDGIIKYEFRICIVSCDEN
                     ILNDIKSNYGIVINSKRSMLVTNTDDDTIVINPKGIIERKIQIDEFENEINLEEGERL
                     DLELNVELEEDVEFIKIDVVYDQSVITIVKQEEKEQPISISGMKTWKLKRERKESFKY
                     LGNSKIVQGTSEYFPKENFKENLQIEKYIIENNIMHCTILEEKYKIIELDVDSRIKEA
                     YKNLISYYKVNDCIPSLAYYDVDLVNISKNYINTILEVFEDIKNGKVLSKERLNIAKV
                     GTIHRIDKEEELFLTPLHPVNVAYQLMINNVIGDEEVKEGILKLLGSKYLMPYIYKGK
                     NSLYKVVDQRHSPEWTYYVDHDMSRYKGSRDYVSKLTKEKINEFIEHFTYLFRDISKS
                     VMKIKLINLGDCKEVLLGIFDYYIKKLGKGEEINNLISIDIYIYGDKSIINSFEEIAF
                     YNSINDIKKHFNVNLSVDNYSEEDVLNLFRDKVKFYKKDKMSDLYEYSHLTLYQMDKD
                     VNITSSSMDDIKTGVALNGLIAGVPSIYLEDTYRTGFGSKFMATTKNELLNISMKFNS
                     LTKSIGTGDPFNDNLSVTTSISENNSSFLNKIYKTSHWVTFIDPKVDLNFFKDDENSN
                     DLLIIHYSDQYTTSSGYDAITVTRKSEQYKFIIEEFLNSKDIKVEEDTIPNLINYFNA
                     INGDWLLRLISSKSQFPREKLSILSAIKISLAYFYHPNIIWIPISLEEILRVSGGAGL
                     KQAGGLFTVKKLTGDTGSYSDDLLLVGIENKNNKIKVYFYPVEVKIGDNNESVIKKAI
                     IQANKTRDLIEEYLTESENDSQKFTKLMYRNFMIQLAIVSAQKMKLYHIWDEQNWDDI
                     INSDIRTNLLNDNYEISNDLDEFIGRGAVISFKKGTYYNGNLGKEGNITILNLSEQEG
                     YNNIVMDLEEMKEKYIRGESDFVIQEMLINKYLDNTIGIQNMDKNVILDTNVMLNKTK
                     DYD"
     gene            4919..6061
                     /locus_tag="KPD58_002821"
     CDS             4919..6061
                     /locus_tag="KPD58_002821"
                     /inference="COORDINATES: protein motif:HMM:NF014044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0266555.1"
                     /translation="MKIIFGNIKNNNKELIWDPTNSIKVPHPNTGIIGTMGTGKTQFT
                     KSLVTQIHREQKHNINGKPIGILIFDYKGDYIKDDFVDATSAKVYEIYHLPYNPLTLI
                     IPERIKPLLPVHTANSLKETIAKAFGLGPKQEVILRELIMKAYEKKGIKKDDENTWVK
                     SCPTMQDVLSIYLDDREIKKDDKLYAALSSFNDFEIFEPNSEKTKNLFDLINGVTVIN
                     LSYYDSGIQNLVVAITLDLFYSQMKALGSSDITGNGEYRQLNKMILVDEADNFLSRNF
                     TSIKNILKEGREFGVGTILSTQLLSHFSTSENEYQNYISTWIIHNVSDINNKDIRYIF
                     NSKNKSEEDNIYNKIKNLAKHESLVKMPSENSPIYIRDKAFWELNK"
     gene            complement(6171..6554)
                     /locus_tag="KPD58_002822"
     CDS             complement(6171..6554)
                     /locus_tag="KPD58_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DndE family protein"
                     /protein_id="HBG0266556.1"
                     /translation="MGFRLKTSIQTKEIFEELGSRMNLKPFALCKLAISISLSNSEPI
                     ENYESIDNNGLELNRQTITNQYDTLFKTLLEQHAGRHLSDDEYFPNQVKLHIDRGALM
                     LKNRYNYSSNLEHFITQIVQENNNL"
     gene            complement(6557..>7824)
                     /locus_tag="KPD58_002823"
     CDS             complement(6557..>7824)
                     /locus_tag="KPD58_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA sulfur modification protein DndD"
                     /protein_id="HBG0266557.1"
                     /translation="LPFLIVKKQILNLEKQLKVENDSILYSHLKDKLNIEYIKNLLSD
                     KIMSSSLDEIALTVSEALIEDIKPDSYEDNFESIHKLSNDESSKLLSLINTILNFDNN
                     SIINSFGEHRSISNELADIRHKLNTSFKDETLNTYINDISSLSDKIDSLLKSKNLLVT
                     SLDKLRLEIAETITKRDKSKAKYIYLLQHNNIVDISSNLILMLDEIITTLTETKIKLV
                     QDNFMYIFKRIIQKDNFISSIDIDANFNISLYINKIYNSLELENLVENIGSEEMEKKL
                     GKLFFEDLFNLYRVKDKDDLFNAIKNNTQSTFLNLRTKVDIDEFSSGEKQIYILCLYW
                     ALLKSSEIEIPFIIDTPYARIDDTHRNNITSEYFSTISDQAIILSTNTEIDEKSYKII
                     KPKLSNEYLIEYDNKNKKTIQNKGYFFEV"
BASE COUNT         3268 a          734 c         1186 g         2636 t
ORIGIN      
        1 tgttttgaat atgaacctga taatattatg gaacttatta tattaaaaaa tcttcctgaa
       61 cttgaaaatg aagagacgtc taaatcaatt tataaatgta tatgtaaaaa tgtgtctaaa
      121 ttattccaag aagatttaga atttatgctt aattatccag aattaattat aaacgagttt
      181 gaaagattat taaagtacta ttattttttc tatgtatcac aattatcaat aaaattatct
      241 cacttttttg atggagatag agatagaact gaaaaagtat attttatttt aagttgggaa
      301 aaagcatcta aaacaagaat aagttataac atgggttgga aaatgttgga gagtcatatt
      361 agaatattat ttagtcatgt tcatacatta gagatattaa atactaataa tgacattagt
      421 aagtatgatt atgtagattt aaataaaatt ataaaaaatt tatcagaaaa tgaaagactt
      481 aggttatatg aaaatataat agatataaaa aatacttatg tcaagtcaat tactgatgta
      541 caatgggatg attatgatga acaaactaaa gtgtataaga atgataacat aaaagaagaa
      601 atatgtgatt tacataaaag aatagattat cagttcaaga aatcttcaag agaggctaac
      661 tataagaggt ataaaaattt atttgaagag tattgtaaat taaatttttt aaaaaaagca
      721 gggccattgg gaaatgtatt gaaaattaat caagaagaac tgttatttat gacaaaatta
      781 tgtatgaaaa ataagacaaa gataaaatta aaggaattat ttgaagaata tgaaaaaaga
      841 ggaatttatt ttgatagaga ttctcaaagc aaagtagtag agttatttga aaaattaaat
      901 ataatagaaa aaaagagtga tagtggggat gcacaatatg tcagatcaat tttatagata
      961 tctagctaaa aatctagtta aatatttcga atctagagaa ataaaaagtg gcgataaatt
     1021 taatatacag tttgaaagag aagaacaagt taaaattatg tatgatgttt tagagaaaga
     1081 aagtaatagt agaccattta catataatat aatgaatgga tcaaaatatc agacttttag
     1141 tatgaatgtc agtggtattc aattaatagt tgcttcaaat aataatacaa ccaatgattt
     1201 tttaattaga cttagaaatc ttataggaaa tgtagctagt gatgaattta aggattcagc
     1261 catattattt atacataata tgacattaga tagtttaata agaggaacag aaagtttgca
     1321 aaaagaaggt atgccatttc atacaaaaac tttagtaaag gacatacaaa ataaattaga
     1381 aaatgataaa ttaaaagaag atattaaaca tatactttat tttgtacttg atagaatgaa
     1441 taaaagtata ttagaagata attcatcaat atttcaatat gaaaatatat tagatatatt
     1501 aaataaagga agaattgaaa agagtgaata tagtaaattt ggattatttt atgatgataa
     1561 tatgttagac aacaaaagta gcaaggcaat aaaagaaaga ctattagata atgaaaagct
     1621 gttttctatt gtaaactcta tacatcagta tggagaccct aagctggaat tagagaaaca
     1681 ttttgattca aaaggtatag atcttttaaa agatgagaat tggaagtatt ctacctataa
     1741 agatataaag tcatctcaag aaagaatgaa aaacaaaaag gtagagttat tatgtataga
     1801 atgtgttgaa aacctaaagc tctggagtaa accagatatt gaaaataagt caaaacgaaa
     1861 aaacatatta ttatttaata aagacaaact tgatattata aatttatgca taaaatttga
     1921 taagaaaatt gaaaaaacaa gtttttctat agataagatg tctaaaacat atacagaagt
     1981 aaaagcaagt ggaaataaag cttatataag tataaatata aatatttcag aaactacatt
     2041 tactaaagtt aaatataatg atggtataat aaaatatgaa tttagaattt gtatagtatc
     2101 ttgtgatgag aatatattaa atgatataaa aagtaattat ggaattgtta ttaattcaaa
     2161 aagaagtatg ttagttacta acacagatga tgatacaata gtaataaacc caaaaggtat
     2221 aattgagaga aaaatacaaa tagatgagtt tgaaaatgaa attaatcttg aagaaggaga
     2281 aaggctagat ttagaactta atgttgaatt ggaagaagat gttgaattta taaaaattga
     2341 tgtagtatat gatcaatctg taattaccat agttaaacaa gaagaaaaag aacaaccaat
     2401 ttctataagt ggaatgaaaa cctggaaatt aaagagagaa cgaaaggaga gttttaaata
     2461 tttaggtaat agtaaaattg tacaaggtac tagtgaatat tttccaaaag aaaattttaa
     2521 agaaaatttg caaatagaaa aatatatcat agaaaataat ataatgcatt gtactatttt
     2581 agaagaaaaa tataaaataa tagaattgga tgtagattcc agaataaaag aagcatacaa
     2641 aaaccttata agttactata aagtaaatga ttgtattcca agcttggcat attatgatgt
     2701 ggatttagtt aacatatcaa aaaattatat aaatactata ttagaagtgt ttgaagatat
     2761 aaaaaatgga aaagtattat ctaaagagag acttaatata gctaaggtag ggactataca
     2821 tagaatagat aaagaagaag aattattttt aacaccatta catccagtaa atgtggctta
     2881 ccaattaatg ataaataatg ttataggtga tgaagaagtt aaagaaggaa tcttaaaact
     2941 attgggttcg aagtatctaa tgccctatat atataaaggt aagaatagtt tatataaggt
     3001 agtagatcaa agacattcac cagagtggac atactatgtt gatcatgaca tgagtagata
     3061 taagggatct agagattatg tctctaaatt gacaaaggaa aaaataaatg aatttataga
     3121 acattttaca tatttattta gggatataag taaatcagta atgaagataa agctaataaa
     3181 tttaggtgat tgtaaagaag tattacttgg tatatttgat tattacataa agaaattagg
     3241 taaaggtgag gaaataaata acttaatatc aatagatatt tatatatatg gagataaaag
     3301 tattataaat tcatttgaag aaatagcatt ttataattca ataaatgata taaaaaaaca
     3361 ttttaatgta aatttaagtg tagacaatta ctctgaagaa gatgtattaa atttatttag
     3421 agataaagtt aaattttata aaaaagataa aatgtcagat ttatatgagt attcccattt
     3481 gactttatat caaatggata aggatgtaaa tataacaagt agctctatgg atgatataaa
     3541 aacaggtgta gcattaaatg gattgatagc aggagttcca tcaatatatt tagaggatac
     3601 ttataggact ggatttggat ctaagtttat ggctacaact aaaaatgaac tgttaaatat
     3661 ttcaatgaag tttaattcac ttacaaaatc aataggaact ggagatccat ttaatgataa
     3721 tcttagtgtg acaacatcaa taagtgaaaa caatagttca tttttaaata aaatttataa
     3781 gacatctcat tgggttactt ttatagatcc aaaagtggat ttaaattttt ttaaggatga
     3841 tgagaattca aatgacttat taataataca ttatagtgat caatatacaa catctagtgg
     3901 atatgatgct ataacagtta ctagaaaatc tgaacaatat aaatttataa ttgaagaatt
     3961 tctaaatagt aaagatatta aagtggaaga agacacaatc cctaatttga ttaattattt
     4021 caatgctata aatggagatt ggttattaag gcttatatct agtaaatctc agtttcctag
     4081 agaaaaatta agcatattat cagctataaa aataagctta gcttattttt atcatccaaa
     4141 tataatatgg ataccaatat ctcttgaaga aatacttaga gtatctggtg gagctggatt
     4201 aaaacaagca ggtggtttgt ttactgttaa gaaactaaca ggagatactg gatcttattc
     4261 agatgattta ttacttgttg ggatagaaaa taaaaataat aaaataaaag tatattttta
     4321 tcctgttgaa gtgaaaattg gagataataa tgaaagtgtc ataaaaaagg caataattca
     4381 ggctaataag acaagagatt taatagaaga gtacttgact gaaagcgaaa atgattccca
     4441 aaagtttaca aagcttatgt acaggaattt tatgatacaa ctcgctatag taagtgcaca
     4501 aaaaatgaag ttatatcata tatgggatga acaaaattgg gatgatataa taaatagtga
     4561 tataagaaca aacttgttga atgataatta tgaaataagt aatgatttag atgaatttat
     4621 aggaagagga gctgtaatat catttaaaaa gggaacttat tataatggga atttaggcaa
     4681 agaaggcaat ataactatac taaatctttc agaacaagaa ggatataata atatagtgat
     4741 ggatttagag gaaatgaagg agaagtatat tagaggagag agtgattttg ttatacaaga
     4801 aatgcttata aataagtatt tagataatac aataggtata caaaatatgg ataagaatgt
     4861 tattttagat actaatgtta tgctaaataa aacaaaagat tatgattgaa gtggtttaat
     4921 gaaaattata tttggaaata taaaaaataa taataaagaa cttatttggg atccaacaaa
     4981 ttcgattaaa gtgcctcatc ctaatactgg tataatagga acaatgggaa ctgggaaaac
     5041 acagtttact aaatctttag taacgcaaat tcatagagaa caaaaacata acataaatgg
     5101 taaaccaata ggaatactga tttttgatta taaaggcgac tacataaaag atgattttgt
     5161 agatgcgaca agtgctaagg tatatgaaat atatcattta ccttataatc cattaacttt
     5221 aataatacct gaaagaataa agccactact acctgttcac acagcgaatt ctttaaaaga
     5281 gacaatagca aaagcatttg gcttaggccc taagcaagaa gttattttga gagaattaat
     5341 tatgaaagct tatgaaaaaa aaggaataaa aaaagatgat gaaaatacat gggtaaaatc
     5401 atgccctaca atgcaagatg tattatcgat ataccttgat gatcgagaaa taaaaaaaga
     5461 cgataaattg tatgctgctc tttcaagttt taatgatttt gaaatatttg agccaaatag
     5521 tgagaagact aaaaatttat ttgatttaat aaatggagtt acagttataa atttatcata
     5581 ttatgattca ggaatacaaa atttagtagt tgcgataact cttgatttat tctatagtca
     5641 aatgaaggca cttggatcaa gtgatataac tggaaatgga gagtatagac aattaaataa
     5701 aatgatatta gtagacgaag ctgataattt cttgagtaga aactttacat ctataaaaaa
     5761 tatattgaag gaaggtagag agtttggagt aggaactatt ctatcaacac aacttctaag
     5821 tcatttttca acttcagaaa atgagtatca gaattatatt tcaacttgga taatacataa
     5881 tgtatcagac ataaataata aagacataag atacatattc aattctaaga ataaaagtga
     5941 agaagataac atatataata agataaagaa tctagctaaa catgaaagct tagtcaaaat
     6001 gcctagtgag aactcaccta tatatattag agataaagca ttttgggaac ttaataaata
     6061 aaataaatga atttagaaaa aatacttata attaaaacat gggataggca attttattta
     6121 ttgtctatcc catgttttaa tttatttatg tttaataaat attaattaat ttatagatta
     6181 ttattttcct gtactatttg agtgataaag tgttctaagt tgcttgaata attatatctg
     6241 tttttaagca taagagcacc tctatctata tgaagcttaa cttggttagg aaagtattca
     6301 tcatcactta aatgtctacc tgcatgctgt tcaagtaaag ttttaaatag agtatcatat
     6361 tgattggtta tagtttgtct attaagttca agaccattat tatcgatgct ttcatagttt
     6421 tcaattggct cactattact tagtgagatt gagatagcta gtttgcaaag agcaaatggt
     6481 ttcaaattca ttctagatcc taattcttca aatatttctt ttgtttgtat ggatgtttta
     6541 agtctaaatc ccataattac acctcgaaaa agtatccttt attttgtata gtttttttat
     6601 ttttattatc atattcaatc aagtattcat tacttaactt aggctttatt atcttatatg
     6661 atttctcatc tatttcagta ttagtagata gtattatagc ttgatcactt attgtagaaa
     6721 aatattcact agtaatatta tttctatgag tatcatctat tctagcataa ggtgtatcta
     6781 taataaatgg tatttctatc tctgaagatt taagtaaagc ccaatataaa caaagaatgt
     6841 atatttgttt ttcaccacta gagaattcgt ctatgtcaac tttagttctt agatttaaaa
     6901 atgtagattg agtattattt tttatagcat tgaatagatc atctttatct tttacacgat
     6961 ataagttaaa taaatcctca aaaaataatt ttcccaactt cttctccatt tcttcagatc
     7021 ctatattttc tactaaattt tcaagttcta aagagttata tattttattt atatataaag
     7081 aaatattaaa gttggcatca atatctatag aactaataaa attgtctttt tgaataatcc
     7141 ttttaaatat atacataaaa ttatcttgaa ctagtttaat tttagtttct gttaatgttg
     7201 ttattatttc atctaacata agtataagat tagaagatat atcaacaata ttattatgtt
     7261 gaagtaaata tatatattta gccttagatt tgtctctttt agtgatagtt tcagctattt
     7321 caagtcttaa tttatctaaa gaagtaacta gcaaattctt tgattttaat aaagaatcaa
     7381 ttttatctga taaactagat atatcattta tatatgtatt caaagtttca tctttaaagc
     7441 tagtatttaa cttatgtctt atgtctgcca attcatttga aatacttcga tgttctccaa
     7501 aggaattaat gatagaatta ttatcaaaat ttaaaatggt atttatcaaa gataataatt
     7561 tactactttc atcattagaa agcttatgga tagattcaaa gttatcttca taactatctg
     7621 gttttatatc ttctattaaa gcctcagaaa cagttagagc tatttcatct aatgaggaag
     7681 acatgatctt atctgaaagt agatttttta tatactcaat atttaattta tctttaagat
     7741 gagaatataa tatactatca ttctctactt taagttgttt ttctaagttt aatatttgtt
     7801 tttttacaat taaaaaggga agta
//
LOCUS       DAELLV010000175         7804 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.175, whole genome shotgun sequence.
ACCESSION   DAELLV010000175 DAELLV010000000
VERSION     DAELLV010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7804
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.175"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(277..1008)
                     /locus_tag="KPD58_002824"
     CDS             complement(277..1008)
                     /locus_tag="KPD58_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266558.1"
                     /translation="MNIFDILRDYLLVQVDKYNLNMDMISIVSKSLSSKEAIGNTKRK
                     DFPIIVGKEIMLEADFKGAKGQAFTSTPSTFEGSLKDILSLDLHDNPHDRSLFIASLN
                     AVMKYLGKTDRTIHCKNNEPEVCAKKFPEFIKMEFGNPKVAIIGYQPAIIDNIKDFFE
                     TRVLDLNPEFVDTIQYNVKIEDGIRDYEDVISWADLVICTGSTLCNNSIINFLSLNKP
                     VYYYGTTIAGASNILGLERLCFCSK"
     gene            complement(995..1690)
                     /locus_tag="KPD58_002825"
     CDS             complement(995..1690)
                     /locus_tag="KPD58_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0266559.1"
                     /translation="MLKIKNLSLDLPNFKLQENSLNINNHEYFVLLGASGSGKTLFLE
                     TLAGRYPSAKGNVSYKENLISNLPPENRNIGFVYQNFELFPNMSVEDNIRFPLKLRKI
                     PIDEQILKANHLSKLMQIEDIKNRYPKNLSGGEKQRVAFARALIAEPDILLLDEPMSS
                     LDYITKKHISKILKDVYIKYKPTVIHVTHDISEAMFFAHKIGIMNNGKINHIFDLNEE
                     IKNKGESFFYEYI"
     gene            complement(1692..2483)
                     /locus_tag="KPD58_002826"
     CDS             complement(1692..2483)
                     /locus_tag="KPD58_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0266560.1"
                     /translation="MKNKFFTTLLTGFFAIVFLFIVSPLLMLIIKGISYVPICLKSVE
                     VQYAITLSIKTSLISTIVCLLLAIPVAYFLHITKLPFKKLIIQIINLPMSLPHLVSGI
                     ALLLLFGRMGIGDSIYKIFKLDFIFTKQGIVLAQIFVNLPLTIKILHTSLNESNEKMI
                     FVARTLGCNSWEAFRFIILPNLKTGIISATVMTWSRALGEFGAVAMIAGSTRMKTEII
                     PTSIYLNMSTGDIDIAIGIAVILIFISLTCLMLFEIFFNREVDKN"
     gene            complement(2493..3305)
                     /gene="modA"
                     /locus_tag="KPD58_002827"
     CDS             complement(2493..3305)
                     /gene="modA"
                     /locus_tag="KPD58_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG0266561.1"
                     /translation="MFKKLSVLALAMIISLSITACNSKENKANDESNTKVTTADDSKE
                     KETLFVYSGAGLKKPMDEIAKKFEKENNVKVEYSYAGSAQLIAQIETSHKGDVFIVGS
                     EPIYSKAFEKELVGEHKTVAHHTPAIVVPKGNPANIQKLEDLNKDGVKLILGDKESNA
                     IGKTTQKILEKNNLKSINDNVVSTAATVNEMIVQLTSSKADATIATKDSVFGNEDVEV
                     IEIAQDKNIDQLISAGIVNYSDKKDLANKFINYIESDEAKQIFKKYGFEPVK"
     gene            complement(3942..4142)
                     /locus_tag="KPD58_002828"
     CDS             complement(3942..4142)
                     /locus_tag="KPD58_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBG0266562.1"
                     /translation="MNNGIVKWFNNEKGFGFISMESGDDVFAHFSAIQTSGFKSLEEG
                     QKVSFDIVKGARGPQAENITIL"
     gene            complement(4367..4543)
                     /locus_tag="KPD58_002829"
     CDS             complement(4367..4543)
                     /locus_tag="KPD58_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266563.1"
                     /translation="MFRDKMDKCTHMLTAYIGSSYDYCDFIDTHLDDFILEYGEKVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(4597..4686)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(4866..5066)
                     /locus_tag="KPD58_002830"
     CDS             complement(4866..5066)
                     /locus_tag="KPD58_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0266564.1"
                     /translation="MPKFTCNLKKYRQLKELTQEQLAEKVGVRRETIMRLEAGKYNPS
                     LKLAIDISKIVDTPIDELFIFD"
     gene            complement(5068..5418)
                     /locus_tag="KPD58_002831"
     CDS             complement(5068..5418)
                     /locus_tag="KPD58_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HBG0266565.1"
                     /translation="MIQKKFFMGFCGFLGFLSLRYFSSGNVADLTYIGFFAFFSNFII
                     AKINGDKADERYVQDEKAAMAFTGQLAIIELFILWCITIVSRNIELMCVLLSITYAIT
                     LNVYAIKLYILEEK"
     gene            5580..5768
                     /locus_tag="KPD58_002832"
     CDS             5580..5768
                     /locus_tag="KPD58_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG0266566.1"
                     /translation="MTKYDFNFKLKVAKFYLNREGRDILIVKQYGVYDHSQVERWVNI
                     LGESELKIKKFSILYGSS"
     gene            complement(6245..6415)
                     /locus_tag="KPD58_002833"
     CDS             complement(6245..6415)
                     /locus_tag="KPD58_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266567.1"
                     /translation="MHVYQPKTSEYLLNTSAKSYLSSIVFLFDYITKNESRYAFFIID
                     FPFLSLDIFYKF"
     gene            complement(6804..7292)
                     /locus_tag="KPD58_002834"
     CDS             complement(6804..7292)
                     /locus_tag="KPD58_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG0266568.1"
                     /translation="MKNKKGFTLVELLVVIAIIGILAIVALPALFKNIEKAKIAKLEA
                     DISAIKSASLSYYADESKYTEGNIIWWTKKDGKITVNSGIGDEDPLAHKIENLGMPYN
                     GSYTLVSSNGSEEYLELNIIIDGEISKSGLDKLEEDYGSSIKIPNDKNMIITFLSNKS
                     DN"
BASE COUNT         2867 a         1064 c          904 g         2969 t
ORIGIN      
        1 caaacaaagt actaaaaatt atttcttaaa tgctgaaaaa tatagagtag attaatggaa
       61 taaaattttt ttattagttt aaaataaaga aaacatttat gtataagact aatcaaaata
      121 ttaatgattg ataggaattg agatttaaag ggattgtaca tttattcggg attcatttta
      181 atagaatttt actttagtac ttagaataaa agtaataact ttttaaattt aaagagtaca
      241 catatatatt tgtgtactcc ttgatgatat gattgcttat ttactacaaa aacagagtct
      301 ttctaaacct aaaatatttg atgcaccagc tatcgtagtt ccataatagt aaactggttt
      361 gttaagtgat agaaaattta taattgagtt attacataat gtacttcctg tgcaaatcac
      421 taaatcagcc caagatatta catcttcata atctcgtata ccatcttcaa tcttaacatt
      481 atattgaata gtatccacaa attcaggatt taaatctaat actcttgttt cgaaaaagtc
      541 cttaatatta tcaattatgg ctggttgata tcctataatt gctacttttg gattaccaaa
      601 ttccattttt ataaactcag ggaatttttt tgcacatacc tctggctcgt tatttttaca
      661 atgaatggtt ctatccgtct tgcctaagta tttcataact gcatttaatg atgctataaa
      721 aagacttcta tcatgaggat tgtcatgtaa atctaatgat aatatgtctt ttaaagaacc
      781 ttcaaatgta gatggagtag aagtaaatgc ttgtccttta gctcctttaa agtcagcttc
      841 aagcattatt tctttaccaa ctataattgg aaaatcttta cgcttagtat ttccaattgc
      901 ttcttttgaa gatagagatt tggacacaat acttatcata tccatattta aattatattt
      961 atcgacttgt actaatagat aatctctcaa aatatcaaat atattcatag aaaaaagatt
     1021 ctcctttatt ttttatttct tcatttaaat caaaaatatg atttattttt ccattgttca
     1081 ttattcctat cttatgagca aaaaacattg cttcagaaat atcatgagta acatgaatca
     1141 ctgttggttt gtattttatg tagacatcct tcaaaatttt gcttatatgt ttttttgtaa
     1201 tataatccaa tgaactcata ggttcatcta aaagtaatat atcaggttca gctattaagg
     1261 cacgtgcaaa agcaactcgt tgcttttcgc ctccacttaa atttttagga tacctatttt
     1321 ttatatcttc tatctgcatt aatttagaca agtggttagc cttaagaatc tgttcatcta
     1381 taggtatttt tcttaattta agtggaaacc ttatattatc ttcaacactc atatttggaa
     1441 ataattcaaa attttgatat acaaatccta tatttctatt ttctggaggt aagtttgata
     1501 ttaaattttc tttgtatgat acatttcctt tagcagatgg atatcttcct gctaatgttt
     1561 ctaggaatag agttttacct gacccactag ctcctaataa aacaaagtat tcatgattat
     1621 taatatttaa actattttct tgtaatttaa aattaggtaa atcaagagat aaatttttta
     1681 tttttaacat attaattctt atcaacctct ctattaaaaa atatctcaaa aagcatcaag
     1741 catgtaagtg agataaatat caaaattaca gctattccga ttgcaatgtc tatatcacca
     1801 gtagacatat ttaaatatat agatgtagga ataatttcag ttttcatacg tgtagaacct
     1861 gctatcatag ctactgcacc aaattcacca agtgctcttg accaagtcat tacagttgca
     1921 gatatgattc cagtttttag atttggaaga attataaatc taaatgcttc ccaactatta
     1981 catccaaggg tacgtgcaac aaatatcatc ttttcattac tttcatttaa tgaagtgtga
     2041 agtattttaa tagtaagtgg aagatttaca aatatttgtg ctaaaactat accttgcttt
     2101 gtaaatataa aatcaagctt aaaaatctta taaatggaat ctccaatccc cattcttcca
     2161 aacaaaagta aaagagcaat tcctgatact aagtgtggaa gagacatagg gagatttatt
     2221 atctgtataa tcagtttttt aaatggtaat ttagtaatat gtaaaaaata tgctactgga
     2281 atagctaata aaaggcatac tatagtagat attaaagatg ttttaatact aagtgttatt
     2341 gcatactgta cttccactga ttttaagcat ataggaacat aacttatccc tttaatgatt
     2401 aacataagaa gtggtgatac aataaacaaa aagactatag caaaaaatcc agtaagtaga
     2461 gtagtaaaaa atttattttt caagtttaat cactacttaa caggttcaaa accatatttt
     2521 ttaaatatct gtttagcttc gtctgactct atataattta taaatttatt agctaaatct
     2581 tttttatcac tatagtttac gattcctgct gatataagtt ggtctatatt tttatcttgg
     2641 gcaatttcta taacttctac atcttcatta ccaaagaccg aatcttttgt ggctattgta
     2701 gcatcagctt tacttgatgt taactgtaca atcatttcat ttacagttgc agctgtagag
     2761 acaacattat catttataga ttttaaatta tttttttcaa gtattttttg tgtagtctta
     2821 cctatagcat tagactcttt gtctcccaaa ataagtttaa ctccatcttt atttaagtct
     2881 tccaattttt gaatattagc tggatttcct ttaggtacaa ctattgcagg tgtatgatgt
     2941 gcaaccgttt tatgttcacc tacaagttct ttttcaaagg cctttgaata aattggttca
     3001 gaaccaacaa taaatacgtc tcctttgtga cttgtctcaa tttgagctat tagctgtgca
     3061 gaacctgcat agctatattc aactttgaca ttattttctt tctcaaattt ttttgctatt
     3121 tcatccattg gtttctttaa acctgcccct gaatagacaa aaagagtctc tttttctttt
     3181 gaatcatcag ctgttgtcac tttagtatta gattcatcat tagctttgtt ttctttagaa
     3241 ttacatgcag taatacttaa tgaaataatc atagctaaag ctagtactga aagtttttta
     3301 aacataagat attccccctt agtaaattag tgtaaattct tttttagtat atcatactaa
     3361 gaaaacgatg tctaagtttt tgttatgttt tataatgttt tgttatgttt tgttatgtat
     3421 agttaagaaa aaatctattt ataatgtcat ttccataggt agttatttta tttttaattt
     3481 ttattaatac tttaaagtat taataaaaat taaaaactta aatcaaatta acttattgta
     3541 gattttttac tttttaacta attttatatt tattatattt cagtgaaaaa atagaacatt
     3601 gaaacatata gatatgtttt aattgttttt tattaacaaa ttactaaaag tgttatatca
     3661 gaattgaata caaaagaaag agtatgtatt tatgagatag ttgaaaattt tgatgtgcca
     3721 caactaaaat tattatatta ctatatattc taattaaaag agtacttgta ggtttaagta
     3781 catactttat aaatatattt ttgtcagatt tattttataa aaagtattta gactaagtat
     3841 tatttttaag tattttcttg ttgaagttat tttttccttt tttacaaaat gcgtaaataa
     3901 aaaaaagact cttaaaagag tctttccgaa aaatctatat attataatat agttatattt
     3961 tctgcttgag gacctctagc gcctttaact atatcaaagc ttactttttg accttcttct
     4021 aatgatttaa atcctgaagt ttgtatagct gagaaatgag caaatacgtc atctccactt
     4081 tccattgata taaatccaaa tcctttttca ttattaaacc attttactat tccattattc
     4141 attaaaaata ctcctattaa cctatatcaa gatatacttg atttacttgt gtattaccac
     4201 aaatactatt gtatcataga ttaacataaa tgtctattta attataattt ttagttaaca
     4261 tttttattta ttcttaatat gaaaataaat ttaaacattt tatattacca atatattaat
     4321 gtaaatgtag ataaaactaa aaagggtttt ctagaaaaaa atctgtctaa ttatacttac
     4381 ttaccaatac catcacttga tgcaagcaag attccacaac tttttctccg tactctaata
     4441 taaaatcatc taaatgtgta tctataaaat cacaataatc atatgaacta ccaatataag
     4501 cagttaacat atgtgtacat ttatccattt tatctctaaa cataatgtat ttatccccct
     4561 aaaaaacttt catgaaaaac aaaaagaaaa tatctttttg gcaactggct gacataactt
     4621 attttataag cgttagtccc tatagctttg cgtcactaaa tttctctagt tttgccgatt
     4681 tagttttatt ctacagatta ataatacaat agaattagtt attattcaac atatctagct
     4741 gaaaaattaa taaatttata tatgaacgtt aaattatctg aaaaaataaa aagcccctct
     4801 gtcaggagtg acttaaaaat tgaatttaag tttgtattta aaacacattt ttaataatgc
     4861 aaaaactaat caaaaataaa caattcatca ataggtgtat ctactatttt tgatatatct
     4921 attgctaatt tcaaagaagg attatacttt cctgcttcta aacgcataat ggtttctctt
     4981 ctaactccta ctttttctgc taattgttct tgagttaatt cttttaattg tctatatttt
     5041 tttaggttac aagtgaattt aggcatatta tttttcctct aaaatataaa gttttatagc
     5101 ataaacattt aaagtgattg catatgtaat tgatagtaaa acacacatta attctatatt
     5161 cctagacact atagtaatac accataaaat gaataattct ataatagcaa gctgtcctgt
     5221 aaatgccata gctgcttttt catcttgtac atacctttca tctgctttat caccatttat
     5281 ttttgctata ataaagtttg agaaaaaagc aaaaaatcca atatatgtaa ggtcagcaac
     5341 attgccagat gaaaaatatc taagacttaa aaatccaagg aaaccacaaa aacccataaa
     5401 aaattttttc tgaatcattt tttatcctcc ttagtaattt taaaagagat atatttatat
     5461 ctttatgata caaatatatc tcttttatat tcttttgtca atagaaaatc agtattatac
     5521 aatatttgta ctgcaccata attgttagag caaaaatcta ctgcttgaga tgtttatgta
     5581 tgactaaata tgattttaat ttcaaattaa aagtagctaa attttatttg aatagagaag
     5641 gaagagatat tttaatagta aaacaatatg gagtttatga ccattctcaa gttgaaagat
     5701 gggttaatat attaggagag agtgaactaa aaataaaaaa attttctata ctatatggtt
     5761 caagttagat gttataaact atatgatgat aactaaatat agtgtacaat aaatcgctaa
     5821 gtattttgga ataaacaata gcacagttat agcaacatga aaataaaaat ttttacatag
     5881 tggtgttgat gccttttcca aaatgaaagg aagaccataa tgaataaaaa caaaaacact
     5941 aaagaaaaaa taaattaaca agagaacaag aattagaata tgaaaataaa tttttaaaag
     6001 atgagcttga gtttttaaaa aagatgtgtg cctcagggaa gccacattaa aaaacagtgg
     6061 tatcattatt aatcaaaaga aagttagatg tattatcaaa aaattagaac ttaaatgcat
     6121 agcatttttt cataaatcac ataaatataa ctcctataaa tgtataattg gaaaagtaaa
     6181 aaataataaa acaaatagaa gatttaaaac atcaatagca tatcagaaaa taacaaatga
     6241 tatgctaaaa tttataaaat atatctaatg ataaaaatgg aaaatcaatt ataaaaaagg
     6301 catatctcga ttcatttttt gttatatagt caaataagaa tactatagat gaaagatacg
     6361 attttgcaga tgtattaagt aagtattctg atgtttttgg ttgatacaca tgcaataaag
     6421 aagttgtgtt tgcagataaa ttagcaaaac tatagtataa gttgcaacaa taaagaaaat
     6481 gaaaattatc atactaaagc ataaggcatg ttacaatttt attagatagg acattaacat
     6541 acactaaagt actttttact ataaagagta cttatttttt tgaaattcat taatgtataa
     6601 actatcaaga atattactca acataattta aaaacaactt gaataggtat ttttaaatta
     6661 tgtgatataa taaaaaatag aagtgtatgc caatacactt ctgtagttat aactaagcat
     6721 tctattcttg aggagtgctt ttttcatttt tataaccaac tctttttact ttaacaataa
     6781 ttatcagacc tatatattta tttttaattg tctgatttat tagataaaaa agttattatc
     6841 atatttttat catttggtat ttttattgaa ctaccataat cttcttctaa tttatctaga
     6901 ccacttttac ttatttctcc atctataatt atgtttaatt ctaagtattc ttcactacca
     6961 ttagatgaca ctaaagtgta cgaaccatta taaggcatgc ctaaattttc tattttatgt
     7021 gccaaagggt cttcatcacc aataccagag tttactgtta tttttccatc ttttttagtc
     7081 caccatatta tgtttccttc agtatatttt gattcatctg catagtagct aagagacgca
     7141 ctttttattg cacttatatc agcttcaagt ttagctatct ttgctttttc tatattttta
     7201 aataatgctg gaagtgctac tattgccaat attcctatta tagcaattac tactaataat
     7261 tccactagag taaatccttt tttgtttttc atttatttcc cctccatata aaattaataa
     7321 tatataaatt tttattttgt tgatttctac tttctatatt ttaccatata tagacaaaaa
     7381 aatcactttc tatgacaaat atactattat tttacaattt atggaaatga tttaaaatga
     7441 catttcttgt tagaaaagat ataagcaatg gattaaaata aagaaaacta cagtcatatc
     7501 aacgccaata atattattta aaagcagatg aaaataaaaa gagggcccag aaaataatgt
     7561 ctatttaatt atacttactt accaatacca taacttgata taaacaagat tatactacat
     7621 ttttttatgt tctagtataa aatcatctaa ccgtgtatct ataaaatcaa aataattata
     7681 tgaactatca gtataataag tcaaaatatg tatacatgaa tacatatttt ttaaaagata
     7741 tatattatta catacctaaa aaactttcat aaaaataaaa ataaaatatg attttagtaa
     7801 cttg
//
LOCUS       DAELLV010000176         7793 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.176, whole genome shotgun sequence.
ACCESSION   DAELLV010000176 DAELLV010000000
VERSION     DAELLV010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7793
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.176"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     misc_binding    90..301
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            464..1753
                     /locus_tag="KPD58_002835"
     CDS             464..1753
                     /locus_tag="KPD58_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBG0266569.1"
                     /translation="MEEEKINYALEQIPENQKRGWVAMFSVLVAIGVDLSSVILGAEL
                     AQSMPMKQAILSVIVGSFFSAILYTTCSLVGSSTSLSTSMITKYVFGEAGAKIFSLVI
                     GVSLLGWFGVQVGFFAQNAQIIIKDIFNLDVSMQILSLIGGLLMMSTAIYGYKAMEKL
                     SVYSVPFLLVLMMLTIFLAFRANGISVDDNMKSTMTFAGGVSLSMSIIIVGAIVSPDI
                     SRWAKSRRDCALSSFLGIQFGNAFMIIVSIVLVKCMGTSDIMRIFITLGIAIPGIIVL
                     TLAQWTTNTSNVYSASLSIALVLKKAPEKVLTIVLGIIATLLAVFGIYEGFIGFLNLL
                     GIVIAPVGGVYTAEYYIVKQELKGFDKGVLYKPIVKRSIVSWIIGILITYLSTYGFIT
                     LTTIAPLDGFIAGFVVQSIIGKVLCSTKNKQEIDKAV"
     gene            1807..2886
                     /locus_tag="KPD58_002836"
     CDS             1807..2886
                     /locus_tag="KPD58_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HBG0266570.1"
                     /translation="MRFLTEESVDKIAVGAAVLGTGGGGDPYVGKLVAKQAIKKYGPV
                     KVISLDELDDDALVVPVSGMGSPVITIEKLLSEVELTTPLEIMEKLLNRKVDVIIPIE
                     IGGINSLMPIAVAAKKGLPILDADSMGRAFPEAQMVTFYLEGYEASPAVMSDEKGNSA
                     ILYPVDGIWSERLARTLTVEMGGSSSISDYNLSGAQVKKAAIGNTLTIAETIGGFLLE
                     NKSDSKGAVENILKELCGYKLFEGKVIDIKRELKGGFTRGHAFFAGINEYDGEYSILF
                     QNENLIAKKGDTPLCITPDLIAVLDLETGFPITTERIKYGSRVMVVAFPCNEKWRTEK
                     GIETVGPGYFGYDVEYKTVEELQGK"
     gene            2907..4451
                     /locus_tag="KPD58_002837"
     CDS             2907..4451
                     /locus_tag="KPD58_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HBG0266571.1"
                     /translation="MYRVGIDVGGTNTDACILDGELKVIHSVKVATTKDVETGVYNAL
                     KKVIDESKIDHSLIKYAMLGTTHCTNAIVERKRLNNVAMIRIGKPATTAILPFIDWPE
                     DLREKIELDSILVSGGYEFDGRKINELCKDEVVEFCNRIKGKVESVAISGVFAPVNKQ
                     QEEEVAKWVREILGDIPISLSNEIGNIGILERENGAILNSALSQVGKAVSLGFEKALK
                     DLGIDAQIYFSQNDGTLMNLEYTMKYPIFTIGCGITNSIRGASFLSKVKNAIVLDVGG
                     TTSDLGVLYNGFPRESSIPVTIGGVHTNFRMPDVLTIGLAGGTCVKGEKDNIKIGPES
                     VGYRITEDAIIFGGDTLTLSDVVTKLDMAFEDRKSNVENLDKEYCNEVYKKVILKLED
                     AVDQMKTSQDDVELVLTGGGSIIVPEKLKGISNVIRPPHYTAANAIGAALGQISGDVE
                     KVYSLDKMSREDAIEDAKKEAVNKALKAGAKEDTIEVLNIEDVPLAYLPGNALLIKVK
                     VVGDLI"
     gene            complement(4614..5156)
                     /locus_tag="KPD58_002838"
     CDS             complement(4614..5156)
                     /locus_tag="KPD58_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HBG0266572.1"
                     /translation="MKSYIALLRGINISGKNKIIMSELKASFVELGYSEVSTYLNSGN
                     VVFLSDVDDSKVISNTIRLMIKKQFELEIPVFIISQEELKDILNNAPNWWGDNNKEIY
                     DNLIFMFPDLSYDEFYDEVGNPKEEYEKVYHYKNAIFWSFSRKDYKKTNWWSKTASSN
                     VSDKITIRTANTVRKIVGMR"
     gene            complement(5304..5588)
                     /locus_tag="KPD58_002839"
     CDS             complement(5304..5588)
                     /locus_tag="KPD58_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG0266573.1"
                     /translation="MAKNTKQTTVHIDETLLKEVKRIGINENKSISQIINESIIDYIL
                     VYRENEDKEKNEHDKNLNETINEFKKEEEKRMQAKSAARAIAEAKKRVLY"
     gene            complement(5651..6415)
                     /locus_tag="KPD58_002840"
     CDS             complement(5651..6415)
                     /locus_tag="KPD58_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBG0266574.1"
                     /translation="MNNYLKFATYQLIGFTSSIPRLIKIKKNPDKFSLKEKFEFMQKQ
                     AKKSLDIVNIELNIIGKETLPKEPLLFVVNHSSMLDSFILTASVERPIGCVIADEPVW
                     RNIPIFKEWAKLLRCVYVNRKNNREGIKSIAQASQNILTGQSMAVFPEGDLTWIKEPN
                     SLVSEFRSGALKIAYKAKCPIVPLVIKNSKDTYEGYQPIGKINSVPVEVEFLEPIYDH
                     IENPRLKSSVLGENIKNKMINTIENFRKSNKTFKEF"
     gene            complement(6697..6963)
                     /locus_tag="KPD58_002841"
     CDS             complement(6697..6963)
                     /locus_tag="KPD58_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBG0266575.1"
                     /translation="MIESRCGILCYECGYKEKVNCKGCTEIEKPFWGEQCPVKSCCED
                     KNLTHCGLCDTFPCEMLNQFAYDKEQGDDGKRISQCKKWAVLAL"
     gene            complement(7005..7760)
                     /locus_tag="KPD58_002842"
     CDS             complement(7005..7760)
                     /locus_tag="KPD58_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0266576.1"
                     /translation="MPHKLLRQYIAHYTISIPDTSIKENLTLIPDASGCMIFKFDKKG
                     IESAFWGATTKTTIVKNDIENVLFRVFVEFRPGGVYYLTGLSQRESTDLKISLEDFNT
                     LFSLEVNSIFERTSTIKELVEQLDMLFLSYLLKSNIVDMTIPILENARKQNSIMSVKN
                     ISQISCYSERHLNRIFNNSLGMSVKSYLRLLRINLVLQEIQNNKIPFATLAQDIGYYD
                     QSHFINDFKSICGVNPTTYIKNLSDFYNEKYKF"
BASE COUNT         2711 a          992 c         1278 g         2812 t
ORIGIN      
        1 taaaaaatgc aagtaattga ctttttaagt tgacaaaata ttgcatatat attatgatat
       61 aaatcataaa ttaactacaa atcaatatta tatattcgta gaagaagaaa gtagcataaa
      121 ggaattttac agagagttag atttggctga gaactaatat tggaatttat gtgaaaagga
      181 ctttggagaa tactattgta attatgttag aatagtggtt taattcgcct taagaaaatg
      241 agtgagtaaa attaattttt actaataaga gtggtaacac ggatttatat tcgtctcttg
      301 gatttttcca agaggcgttt tttattataa acttatttca gaaaatttaa aactatatat
      361 taaatgaaaa ttataaaaat atgatagtta ataaatttat aaattagaaa attgtaaaat
      421 atgctaaggt cagtagtttt acgatttaaa tggagggatg aaaatggaag aagaaaaaat
      481 taactatgct ctggaacaga ttccagagaa tcaaaaacgt ggttgggtag ctatgttttc
      541 agttttagta gcaataggtg tggatttatc atctgttatt ttaggcgctg agttggcaca
      601 gagtatgcca atgaaacaag caatattgtc agtaattgtt ggttcatttt tttctgcaat
      661 tttgtatact acatgctctt tggttggttc atctacaagt ttatctacat ctatgataac
      721 aaagtatgta tttggagaag ctggagcaaa aatattttct ctagttatag gggtttctct
      781 attaggttgg tttggagttc aagtaggttt ctttgctcaa aatgcacaga taataataaa
      841 agatatattt aatttagatg tcagtatgca gattttaagc ctaataggtg gtctcttgat
      901 gatgagtact gcaatttatg gatataaggc aatggagaag ttgagtgtgt attcagtacc
      961 atttttatta gttcttatga tgctaactat attcttagct tttagagcaa atggaatttc
     1021 agttgatgat aatatgaaat ctactatgac atttgcaggt ggagtttctc tgtcaatgag
     1081 tataataata gttggtgcaa ttgtatctcc tgatataagt cgttgggcta aaagtagaag
     1141 agactgtgct ctttcaagct ttttgggaat acaatttgga aatgctttta tgataatagt
     1201 ttcgatagta ttagtaaaat gtatgggaac ttcagatatt atgagaattt ttattacatt
     1261 aggaattgct ataccaggta taatagtact tacattagca caatggacta caaatacaag
     1321 taatgtatat tcagcatcat taagtatagc tcttgtactt aagaaggctc ctgagaaagt
     1381 attaactata gtccttggta ttatagcaac attattggct gtatttggaa tatatgaagg
     1441 ttttatagga tttttaaatt tacttggaat tgtaattgca cctgttggtg gtgtatatac
     1501 agcagaatat tacatagtaa agcaagaact taaaggcttt gataaaggag tattatataa
     1561 accaatagtt aaacgttcaa tagtgtcttg gataattggt atattaataa cttacctttc
     1621 aacatatgga tttataactc ttactactat agcaccttta gatggtttta tagcagggtt
     1681 tgtagtacaa tctattattg gaaaagtgct ttgttcaaca aaaaataagc aagaaataga
     1741 caaagcagta taaagaaatt tgtttataaa gtataaaata aattagaaat atgaggagaa
     1801 agtaacatga gatttttaac agaagaaagt gtagataaaa tagcagttgg tgcagcagtg
     1861 cttggaactg gaggtggagg tgacccatat gtaggtaaat tggttgcaaa acaagccatt
     1921 aaaaagtatg gtcctgtaaa agtaataagt ttagatgaac ttgatgatga tgctttggta
     1981 gttccagtat ctggtatggg ttctcctgta attacaatag aaaaattact ttcagaagta
     2041 gagttaacta cccctttaga aataatggag aaattattaa atagaaaagt ggatgttata
     2101 attcctatag aaataggagg gataaactca cttatgccta tagcagttgc tgctaaaaaa
     2161 ggacttccta tcttagatgc agattctatg ggtagagctt tccctgaagc tcaaatggta
     2221 actttttatc ttgaaggata tgaggcatct cctgccgtaa tgtctgatga aaaaggtaat
     2281 tctgccatcc tttatcctgt ggatggaatt tggtcagaaa gattagctag aactttaact
     2341 gtagaaatgg gaggaagttc ttctattagt gactataatt taagtggagc tcaagttaaa
     2401 aaagctgcta taggtaatac tcttacaata gctgaaacta taggagggtt tttacttgaa
     2461 aataaatctg attcaaaagg tgctgttgag aatatactaa aagaattgtg tggatacaag
     2521 ttatttgaag gaaaagtaat tgatattaag agagagttga aaggtggatt tactagagga
     2581 catgcctttt ttgctgggat taatgagtat gatggagagt attctatatt attccaaaat
     2641 gagaatttaa ttgccaaaaa aggtgataca ccactttgta ttacaccaga tttaatagca
     2701 gtattagatt tagaaactgg atttccaata actacagaga gaataaaata tggctctcgt
     2761 gttatggtag tagcttttcc ttgtaatgaa aaatggagaa cagaaaaagg aattgagaca
     2821 gttggacctg gatactttgg atatgatgtg gaatataaaa cagtagaaga attacaagga
     2881 aaataaaagt ttaggaggaa attttgatgt atcgtgtagg aattgacgtt ggaggaacaa
     2941 atacagatgc ttgtatactg gatggggaat taaaagtaat tcattcagtt aaggtggcaa
     3001 ctacaaaaga tgttgaaact ggtgtttaca atgccttaaa aaaagttata gatgaaagta
     3061 agattgacca ttcattaata aaatatgcta tgcttggtac aactcattgt acaaatgcta
     3121 ttgtagaaag aaaaagactt aataatgtgg ctatgataag aataggaaag ccggccacta
     3181 ctgccattct tccatttata gattggccag aagacttaag agaaaaaata gaattagatt
     3241 caatattggt atctggtgga tatgagtttg atggcagaaa aataaatgaa ctttgtaaag
     3301 atgaagttgt agagttttgc aatagaataa aaggcaaagt ggaaagtgtt gcaataagtg
     3361 gagtatttgc tccagtaaat aaacaacaag aagaagaagt tgcaaaatgg gttagagaaa
     3421 ttttagggga tattcctata tctttatcaa atgaaatagg taatataggt atattagaaa
     3481 gagaaaatgg agctatttta aattctgcat tgtctcaagt aggtaaagct gtatctttag
     3541 gatttgaaaa agcacttaaa gatttaggta tagatgctca aatatatttc agtcaaaatg
     3601 atggaacatt aatgaattta gagtatacaa tgaaataccc aatttttaca attggatgtg
     3661 gaataacaaa ttctataaga ggagcttcct tcttatcaaa agtaaaaaat gcaatagtat
     3721 tagatgttgg tggaactaca agcgacttgg gtgtactata taatggattc cctagagaat
     3781 catctattcc agttacaatt ggaggagtac atacaaactt tagaatgcca gatgttttaa
     3841 ctattggtct tgctggtggt acatgtgtaa aaggcgaaaa agataatata aagataggtc
     3901 ctgaaagtgt gggatataga ataactgaag atgcaataat atttggtgga gacactctaa
     3961 ctttatcaga tgttgttaca aaattagata tggcgtttga agatagaaaa agcaatgttg
     4021 aaaacttaga taaagagtat tgtaatgagg tatataagaa ggttatttta aaattagaag
     4081 atgctgtaga ccaaatgaag actagtcaag atgatgttga attggtttta actggtggag
     4141 gaagtataat tgttcctgaa aaactaaaag gcataagcaa tgttataaga cctcctcatt
     4201 atactgctgc aaatgctatt ggtgcagctt taggacaaat aagtggagat gtagaaaaag
     4261 tatacagttt agataaaatg tcgagagaag atgccataga agatgcaaaa aaagaggctg
     4321 taaataaagc tctaaaagct ggtgctaaag aagatactat agaagtatta aatatagaag
     4381 atgttccact tgcatacctt cctggtaatg cacttcttat aaaagtaaaa gttgttggtg
     4441 atttaattta aagaataaat ataagtattt ccagaattat ataaatattt ctggactcat
     4501 ttttaaactt attttatcaa taaaaacagc ataacttttg actatattct tataagtttc
     4561 cgaacagccg atatatacct attttttgag gtttatcggc tgtttgttat cttttatcgc
     4621 attccaacta tttttcttac cgtatttgct gtacgaattg taatcttatc acttacattt
     4681 gaactggcag tctttgacca ccaatttgtc tttttataat ctttacgact aaaagaccaa
     4741 aagatagcat ttttataatg atatactttt tcatactctt ctttaggatt tccaacctca
     4801 tcataaaact cgtcataaga taagtcagga aacataaaaa taagattatc atatatttct
     4861 ttgttgttat ctccccacca attaggagca ttatttagta tatcttttaa ctcttcttgt
     4921 gaaataataa aaactggtat ttccaattca aactgttttt ttatcattaa tcttattgta
     4981 tttgagataa ccttgctgtc atctacatca cttaaaaaga caacattacc actattaagg
     5041 tatgtagaaa cttctgaata accaagttct acaaagcttg cttttaattc agacataatt
     5101 atcttgttct taccacttat attgatacct cttaataatg cgatataact tttcatataa
     5161 taatttcacc tctaaacatt cacatttttc tatgtagtac aaacttgaat ttgcatcttt
     5221 tataattctt tcaaaacttc ttcatatgta atttttttaa tataaaaaaa tcggtgctta
     5281 atttgtaccg atttttgttt ttcctagtaa agaactcgtt tttttgcttc tgctatagct
     5341 ctagcagcac tttttgcttg cattcttttt tcttcttctt ttttaaattc atttattgtt
     5401 tcatttagat tcttatcatg ttcatttttt tctttatctt cgttttctct gtatactaat
     5461 atataatcta ttattgattc gtttattatc tgagatatac ttttattttc attaattcct
     5521 attctcttaa cttccttaag tagagtctca tcaatatgaa ccgttgtttg ctttgtattt
     5581 tttgccacct taatcacctc ttaaaaaaaa ttctaacatt aaaatttttt ttagtcaata
     5641 aattttaata ttaaaattct ttaaatgttt tatttgattt tctaaaattt tctattgtat
     5701 ttatcatctt attttttata ttttcaccta gtacagaaga ctttagtcta ggattttcga
     5761 tatggtcata aattggttct aaaaattcaa cttcgacagg aacagagtta attttaccaa
     5821 taggttggta tccttcataa gtatcttttg aattttttat tacgagtgga acaatagggc
     5881 attttgcttt atatgctatc tttaaagctc cacttctaaa ctccgaaacc aatgaatttg
     5941 gttctttaat ccatgttaaa tctccttctg gaaacactgc catactttga cctgtaagta
     6001 tattttgaga ggcttgggct atactcttta taccctcacg attgtttttt ctgtttacat
     6061 atacacatct tagtaatttt gcccattctt taaatatagg tatatttctc caaactggtt
     6121 catctgcaat tacacatcca attggtcttt ctacacttgc tgttagtatg aagctgtcaa
     6181 gcatactaga atgatttaca acaaatagaa gtggttcttt tggcagtgtt tccttaccaa
     6241 ttatattaag ctctatattc actatatcta aagatttttt agcttgtttt tgcataaatt
     6301 caaatttttc ctttaagcta aatttatcag gattcttttt aattttaata agcctaggta
     6361 tagaagatgt aaaacctata agttgatatg tagcaaattt taagtaatta ttcataaaat
     6421 tatctcctta aaataaaatt atttattact gtaaagattt taacataaaa taaaatttaa
     6481 gtctatttta attcaatttt gtaacatata ttttaaatag acagagattg agattttata
     6541 tttccaattt tagtaatcag attttatcaa taaattacta ttctattaat ttaattaaat
     6601 aacctttaaa tttaattata aaaactgttg cagaaatttc tacaacagtt taaaaaattt
     6661 accttacaaa aacttatttt ataacaagag agtatattat aaggctagta cagcccattt
     6721 tttgcactga gaaattcttt tgccatcatc accttgttct ttgtcatatg caaattgatt
     6781 aagcatttca catggaaatg tatcacatag accacaatgt gtaaggtttt tatcttcaca
     6841 acaagatttt acaggacatt gttcacccca aaatggtttc tcaatctctg tacaaccttt
     6901 acaattgact ttttccttgt atccacattc ataacataaa attccacatc ttgattctat
     6961 cattttaagt cactccttaa atataaattt tatatgattg tatatcaaaa tttatacttt
     7021 tcattgtaaa aatcggacaa attttttata taggttgttg gattaacacc acaaatagat
     7081 ttaaaatcat ttataaaatg agattggtcg taataaccaa tatcttgtgc taaagttgca
     7141 aatggtattt tattattttg tatttcttgt aatacaaggt ttatacgaag taatctaagg
     7201 tatgatttaa cactcatacc aagagaatta ttaaaaattc tatttaaatg tcgttcacta
     7261 taacaactaa tttgtgaaat gtttttaaca gacataatag agttttgttt tctagcattt
     7321 tcaagaattg gtatagtcat atctactata tttgatttca ataagtatga taagaataac
     7381 atatctaatt gttctactaa ttcctttata gtacttgttc tttcaaaaat ggaattaacc
     7441 tctaagctaa ataatgtgtt aaaatcttca agtgaaattt ttaaatctgt agattctctt
     7501 tgagaaagac ctgtcaaata atatactcca cctgggcgaa actcaacgaa tactctaaaa
     7561 agtacatttt ctatatcatt ttttacaata gtagtttttg ttgtggctcc ccagaatgcg
     7621 ctttctatac cttttttgtc aaatttaaat atcatacatc cactagcatc aggtattaat
     7681 gtaagatttt ctttaatact tgtatctgga atagatattg tatagtgtgc tatatactgt
     7741 cttaacaatt tatgtggcaa aatatatgta tactgtttgc aggtatttaa aat
//
LOCUS       DAELLV010000177         7779 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.177, whole genome shotgun sequence.
ACCESSION   DAELLV010000177 DAELLV010000000
VERSION     DAELLV010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7779
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.177"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            31..2979
                     /locus_tag="KPD58_002843"
     CDS             31..2979
                     /locus_tag="KPD58_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0266577.1"
                     /translation="MRQRIKNLIENEDKKNPLTDEVISSRLNIRREDVTFLRNELKIE
                     DSRQRRKVVLIKAIKDILKEEKTINKNDITRRLNSIGFTVSRFTVIQYLKEIQDSGDL
                     KLDKESDDKKEEIINNRENKDIAFDKLIGSSGSLSTIIKLAKAAILYPPHGLHTIIMG
                     PTGVGKSELAECMYKFAVESSRFPKNAPFIVFNAADYSENPQLLLSQLFGHVKGAFTG
                     ADENKEGLVSKADGGILFIDEIHRLPHEGQEILFQLIDKGMVRKLGETRLTHKIDVMI
                     ISATSEPIDSHLLNTFKRRIPVTIEIPELVARPLNERFDIINNFFLIEAQRMGANIHI
                     KSDVLKALMLYDCIGNVGQLRSDIQVACARGLLNQLTNKLKEVNISISDVPGYVKQGL
                     IKIRNCRGKIENYVDGDLIIDSNLQGEIEKKNEDIYTFPDEIYKLTERRHIDLLEQGL
                     DFDVINRIIGGELESCIQKYIKQVKKVSISNNLPDISPIVGKYIVELTYEVIKIASQY
                     LSSLEPNLHLCLAVHLKAAFERLKEGKVISNAHLKEIIGNYPVEYSVAEKVAQYLKER
                     YNINLPKEEIGIIAIYFKMSSRRDMSEQKKIGVLLMAHGRVASSVCEVTNKLLGINHA
                     RYLDMPFEKKPEEMIEEATTIIKDIDEGRGVIILVDMGSLSYFGDIISGKTGINIRTI
                     SRFDTLLSIEVIRRAVLPDLTIDEIVDEVKGIEPQVKEKVSKCNNTKVDFRKPIILTL
                     CITGLGGACKIKKILEENIKSITEKVDIEPIGMIEYPSLDLEIKQISNDKNVIAIVGT
                     IDPQIANIPFFSLVDINDKAKLNQINNLIDTNQIGETNKKYELSDLIHKEDIFVDLQV
                     KSKEEVLKYMSKKLREKGYVTKNFYKKVVERDNLFPTELSDEIAIPHTDGIDVIRPAI
                     SIAILKKPIVWSKSKVKIILLLAVDQKCFNPLSTLLSFIETDEFKKIKNIKDRDFIRE
                     MILDGVNENN"
     gene            2957..3421
                     /locus_tag="KPD58_002844"
     CDS             2957..3421
                     /locus_tag="KPD58_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0266578.1"
                     /translation="MESMKIISSNLIFKNIEVSNNEDALKFLGQRLFDEQYVKESYIQ
                     AVVAREKKYATGLPTEIYGVAIPHTDIVHVNEPGIAIGILNKPVKFIMMGTDDTEIDV
                     KVVFMLAVKEPQEQLQLLEKLMTIFQDKNILNNIVDLSEESVSDLLNSKLKN"
     gene            3534..3836
                     /locus_tag="KPD58_002845"
     CDS             3534..3836
                     /locus_tag="KPD58_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0266579.1"
                     /translation="MGVTNVAKKILVACGTGVCTSTIAINKLKKALQDIGKLDMVNIT
                     QCKVVEVASKAPDYDLIICTTQVSSSIKTPVINGLPFLTGVGMDKLINDVLEELEL"
     gene            3898..5262
                     /locus_tag="KPD58_002846"
     CDS             3898..5262
                     /locus_tag="KPD58_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425524.1"
                     /note="with GatAB forms a phosphoenolpyruvate-dependent
                     sugar phosphotransferase transporter for galactitol;
                     subunit IIC forms the translocation channel and contains
                     the substrate binding site; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBG0266580.1"
                     /translation="MEGLYMEILNFIVGLGAQVMMPIIICIFGLILGTKLGKSLRAGL
                     TVGVGFIGLNTIIALLTDNLGPATQQMVKNLGLSLSIIDVGWPAASAIAFASTVGALI
                     IPIGLVVNIVMLITNTTQTVDVDIWDYWHFAFTGALVAGATQSVMWGVFAAVANMVIV
                     LVMADLTAPGIEEYLGMPGISLPHGFTQAFVPIAIVVNKLLDLIPGINKIEINADTLQ
                     KKFGLFGEPLIMGSVIGVIIGIAAKYDIKGILQLGVTMGAVLILIPKMAALLMEGLLP
                     VSEAAQEFIEKRFKNRGKIYIGLDSAVGIGHPVTLSVALVLVPLTILIAAILPGNKVL
                     PFADLAVIPFALVLIVPITKGNVFRTLIIGIIIITSGLLIATNLAPLFTQMALNASFK
                     MPEGATMISSICDGANPLSWVFVKVMNYKVIGGVVFGVIALGMAIYNRNRIINENKKL
                     SIEE"
     gene            5297..5770
                     /locus_tag="KPD58_002847"
     CDS             5297..5770
                     /locus_tag="KPD58_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266581.1"
                     /translation="MKKYKYDSKRYLLSVTIPGFFMILILIYALFNNYTNFNLNIYSL
                     LIIVCTYSIFNTFISSSNPKKIIIDSKCIHFTSFMTTHKYELKDIEKIRIREFYNKKI
                     YLKINDGNLFKGRYWIRTNMFNNSIALYSYFIELEECLYPDSLKFRNKKFENKNI"
     gene            5942..6592
                     /locus_tag="KPD58_002848"
     CDS             5942..6592
                     /locus_tag="KPD58_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBG0266582.1"
                     /translation="MLLEKLRKEVLQASLDLLNYNLVTLTGGNVSGRDEQTGYIAITP
                     SGMDYRNLTPSDIVIVDVDGNIIDGKWKASVDLSDHLYIYKHREDINSIIHTHSTYSS
                     CFAILNEPIECASTTLANEVGGSVPVAKFSPPTSKKMGKCVIEAIGDKRACLLANHGV
                     IAVGPSVGHALTAAVMLEDSAKVYYLAKSIGTPVLLPDEEIQRARDVFFNVYGQDK"
     gene            6766..7698
                     /locus_tag="KPD58_002849"
     CDS             6766..7698
                     /locus_tag="KPD58_002849"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBG0266583.1"
                     /translation="MYYLLNKSTNPYFNLALEEYLFLNDKYNDDIIIIWRNEESIFIG
                     KNQNPYQEVYHDVIEKGEIPILRRISGGGTVYHDLGNINMSFIQKDRQLHEIDFLEHT
                     KFMQKMLSTLGLDVSITERKDLFLNGKKISGSAQSIKRKNSLYHGTLLYDSDLNKLTK
                     YLNSNKATESNATKSVSSKVTNIKFLLEKDINYFLDYCVEYLKSNIKNIKELELDEED
                     IKSVYNIGEDKYKKIEWTYGKTPKFQVVLPFNNESKINIHVNRWKVSKFFISYDDNVI
                     NMDKLLDLNFFKEEIKESILENYPHYIDLINLIF"
BASE COUNT         3179 a          830 c         1350 g         2420 t
ORIGIN      
        1 atataaataa taaggaggga acaattataa atgaggcaaa gaataaaaaa cttaattgaa
       61 aatgaagata agaaaaatcc tttaacagat gaagtgattt caagtaggtt aaatattcga
      121 agagaagatg ttaccttttt gagaaatgag ttaaaaatag aagattcaag acaaagaaga
      181 aaagttgttt taattaaagc tataaaagat attttaaaag aagaaaaaac tataaataaa
      241 aatgatataa caagaagatt aaacagcatt gggtttacag tatcaagatt tacagtcata
      301 caatacttaa aagagattca agattctggt gacctaaaac ttgataaaga gagtgatgat
      361 aaaaaagaag aaataatcaa taatagagaa aataaagata tagcatttga taaactcata
      421 ggtagtagtg gtagcttaag tacaattata aaactagcca aagcagcaat tctttatcca
      481 cctcatggtc ttcatactat tattatgggg ccaactggtg ttggaaagag tgagttagca
      541 gagtgtatgt ataaatttgc agtagaatca tccagattcc ctaagaatgc accatttata
      601 gtttttaatg cagcagatta ttcagagaat ccacagcttt tgctatcaca gttatttgga
      661 cacgttaaag gtgcttttac aggagctgat gagaacaaag aaggattagt atcaaaggca
      721 gatggaggaa tattgtttat agatgaaata catagattac ctcatgaagg tcaagaaata
      781 ctatttcaac tgattgataa aggtatggtc agaaagttag gagaaacaag actaacacat
      841 aaaatagatg taatgattat ttcagcaact tctgagccaa tagattctca tttacttaat
      901 acatttaaaa gacgtatacc tgtaacaatt gaaataccag aattagttgc aagaccatta
      961 aatgagaggt ttgacattat aaataatttc ttcttaatag aagctcaaag aatgggagca
     1021 aacatacata taaagtcaga tgtattaaaa gctcttatgc tttacgattg tataggtaat
     1081 gtaggtcaac ttagaagtga tattcaagta gcttgtgcta ggggactatt aaaccaactt
     1141 acaaacaaac taaaagaagt aaatataagc atatcagatg tacctggata tgtaaaacaa
     1201 ggtcttataa aaatcagaaa ttgtagaggc aaaatagaaa attatgtaga tggggattta
     1261 attatagatt caaatcttca aggtgagata gaaaagaaaa atgaagatat atatactttt
     1321 ccagatgaaa tatacaaact tactgaaaga agacatattg atttattgga acaaggttta
     1381 gattttgatg ttataaatag aatcattgga ggagaattag aaagttgcat tcaaaaatac
     1441 ataaaacaag ttaaaaaagt ttctattagt aacaatcttc cagatataag tccaattgtt
     1501 ggaaaatata tcgtagagct aacttatgaa gtaatcaaaa tagcatcaca atacctaagt
     1561 tcactagaac caaatctaca tttatgttta gcagttcacc taaaagcagc ttttgagaga
     1621 ttaaaagaag gaaaagtaat atcaaatgct catttgaaag aaataatagg aaattatcca
     1681 gtagaatatt ctgtagcaga aaaggtagct caatatttaa aggaaagata taacataaac
     1741 ttaccaaaag aagaaatagg tataatagca atatatttta aaatgagtag tagaagagat
     1801 atgtctgaac aaaaaaagat aggcgtactt cttatggctc acggaagagt agcatcttct
     1861 gtatgtgagg ttacaaataa actattagga attaatcacg caaggtattt agatatgcca
     1921 tttgagaaga agccggaaga aatgattgaa gaagctacta caataataaa agatattgat
     1981 gaaggtagag gtgtgattat attagtagat atgggttcac tttcatattt tggagatatt
     2041 atttctggaa agactggaat taatataagg acaatatcta gatttgatac attgctttct
     2101 atagaggtta tacgaagagc agttttacca gatttaacaa tagacgagat agttgatgaa
     2161 gttaaaggta tagagccaca agtaaaagaa aaggttagta aatgtaacaa tacaaaagta
     2221 gattttagaa aacctataat tttaacatta tgtattactg gattaggggg agcttgtaag
     2281 ataaaaaaaa tcttagagga aaatattaaa agtataactg aaaaggttga tatagaacct
     2341 ataggtatga ttgaatatcc aagtcttgat ttagagataa aacaaataag caatgataaa
     2401 aatgtaatcg ccatagttgg aacaattgac cctcagatag caaatatccc tttttttagt
     2461 ctagtagaca taaatgataa agcaaagtta aatcaaataa ataacctaat tgatacaaat
     2521 caaattggtg aaacaaataa aaaatatgaa ttatcagatt taatacataa agaagatata
     2581 tttgtggatt tacaggttaa atcaaaagaa gaagttctaa aatatatgag taaaaaactt
     2641 agagagaaag gttatgtgac aaaaaatttc tataaaaaag tagtggaaag agataactta
     2701 tttccaactg aattatcaga tgaaatagca atacctcaca cagatggaat agatgtaatt
     2761 aggccagcta taagtatagc catcttaaag aagcctattg tgtggagtaa aagtaaagta
     2821 aaaataatac ttttattagc tgtagaccaa aagtgtttta atccattaag cactctactc
     2881 tcatttatag aaacagatga atttaagaag ataaaaaaca ttaaagatag agattttatt
     2941 agggagatga tattagatgg agtcaatgaa aataattagt agcaatttaa tttttaaaaa
     3001 tattgaagtg tctaacaatg aagatgcatt aaagtttttg ggacaacgtt tgtttgacga
     3061 acagtatgta aaagaaagct acatacaagc tgttgtagca agagaaaaaa agtatgctac
     3121 agggttacca acagaaatat acggagtggc aataccacat acagatatag tacatgtaaa
     3181 tgagccagga atagcaattg gaatactgaa taaacctgta aaatttataa tgatgggtac
     3241 agacgatact gaaattgatg taaaagttgt atttatgtta gcagtgaaag agccacaaga
     3301 acaattgcaa ctattagaaa aacttatgac tatatttcaa gataagaata tactaaataa
     3361 tattgttgac ttaagtgaag aaagtgtgag tgatttatta aactctaaat tgaaaaatta
     3421 aacatgtaaa gtatattaaa aatgtaaaat taaacatgca aagtatgtta aaaaatataa
     3481 aaagattatt aaagatttct aactatagat atctttaaaa taaaataatt tcaatggggg
     3541 tgactaatgt ggctaaaaag atattagttg catgtggaac aggagtatgt acttcaacaa
     3601 ttgctataaa caaattaaag aaagcacttc aagacattgg taaattggat atggttaata
     3661 ttacacaatg taaagtagta gaagttgcat caaaagcacc tgattatgat ttaataattt
     3721 gtactacaca agtatctagt agtataaaaa caccagtgat aaatggatta ccttttttaa
     3781 ctggtgtcgg aatggataaa ttaatcaatg atgtattaga agagttagaa ttataattaa
     3841 aaaaattaag gaacatggtc tctaaaatca taagttttag agacttaaaa ttaaataatg
     3901 gagggtcttt atatggagat attaaatttc attgttggtc taggtgcaca agtaatgatg
     3961 ccaataataa tatgtatttt tggtttgata ttaggtacaa aactaggaaa atctttaaga
     4021 gctggtttaa ctgtgggagt tgggtttata ggtcttaata ctattatagc tttgctaaca
     4081 gataatttag gtccagctac acaacaaatg gttaagaatt tagggctaag tttatcaata
     4141 atagatgtag gatggccagc agcatcagct atagcttttg catcaacagt aggggcttta
     4201 attattccaa taggtcttgt ggtaaatata gttatgctaa ttactaatac tactcaaact
     4261 gttgatgtag acatatggga ttactggcat tttgctttta caggagcttt agtggcaggg
     4321 gctactcaaa gtgtaatgtg gggagtattt gcagcagtag caaatatggt aattgtattg
     4381 gtaatggcag atttaactgc tccaggtata gaggaatatt taggtatgcc aggtatatct
     4441 cttcctcatg gatttacaca ggcctttgtt ccaattgcta tagtagtaaa taaattatta
     4501 gatctaatac caggtataaa taagattgag ataaatgctg atacattgca gaaaaaattt
     4561 ggattatttg gagaaccttt aataatgggg tctgtaatag gtgtaataat tggtattgct
     4621 gcgaaatatg atataaaagg aatattacaa ttaggtgtaa ctatgggagc tgtattaata
     4681 ttaattccaa agatggcagc attattaatg gaaggattat taccagtatc agaagctgca
     4741 caggaattta ttgagaagag atttaaaaac agaggcaaga tttatatagg cttagattca
     4801 gcagtaggaa taggtcatcc tgttacatta tcagttgcac tagtgttggt tcctttgact
     4861 atacttatag cagctatatt gccaggaaat aaagttttac catttgcaga tttagcagta
     4921 attccatttg cacttgtatt aatcgtacca attacaaagg gaaatgtgtt tagaacatta
     4981 ataataggaa taattataat tacttctgga ttattaatcg caactaactt agctccatta
     5041 tttacccaaa tggctttaaa tgctagtttt aaaatgcctg aaggagcaac tatgatttca
     5101 agtatttgtg atggtgcgaa tccattaagt tgggtatttg taaaagttat gaactataaa
     5161 gttattggag gagtagtttt tggagttata gctttaggaa tggctattta caataggaat
     5221 agaataataa atgaaaataa aaagctatct atagaagaat aaggttaaaa caattttatt
     5281 aaggagtgta taggttatga aaaaatataa atatgattct aaaagatact tactcagtgt
     5341 tacaatacca ggatttttta tgattttaat attgatttat gcactgttta ataactatac
     5401 aaattttaat ttgaatatat atagtttgtt aataattgtc tgtacttata gtatattcaa
     5461 tacttttata tcatcaagta atcctaaaaa aattattatt gatagcaaat gtatacattt
     5521 tacctcattt atgactacac ataagtatga acttaaagat atagaaaaga taagaataag
     5581 agaattttat aataaaaaaa tttatctaaa aattaatgat ggaaaccttt ttaaaggtag
     5641 gtattggata agaacaaata tgtttaataa tagtatagca ttatatagct atttcattga
     5701 gttggaagag tgcttatatc cagattcact aaaatttaga aataaaaaat ttgagaataa
     5761 gaacatataa aaataaaaaa ttgaattgaa gcctagctta atcttttttg ataatatata
     5821 agtttgaact ttatactaaa tttttagaat tttcaatcaa gataataaca taatagctaa
     5881 gcaattagaa cacaattaag atgtataagg catgtatatg atgaatggga ggatttaatt
     5941 aatgttatta gagaaattaa gaaaagaagt attacaagca tctttagacc ttttaaacta
     6001 taatttagtg acattaacag gaggaaatgt aagtgggaga gatgaacaaa ctggctatat
     6061 agcaatcacc ccaagtggaa tggattatag aaatcttaca ccaagtgata ttgttatagt
     6121 agatgtagat ggaaatatta tagatggaaa atggaaagca tcagtggatt tatcagacca
     6181 cttgtatata tataagcata gagaagacat aaatagtata attcatacac attctacata
     6241 ctcaagttgt tttgctattt taaatgaacc tatagaatgc gcaagtacaa ctcttgcaaa
     6301 tgaagtaggg ggttcagtac cagttgcaaa attttcacca ccaacatcta aaaagatggg
     6361 aaaatgtgtt attgaagcaa ttggtgacaa aagagcatgt cttttagcaa atcatggcgt
     6421 aattgcagtt ggtccatctg taggacatgc tcttacagcc gctgtaatgt tagaagattc
     6481 agcaaaagta tattacttag caaaatcaat tggcacacca gtgctactgc ctgatgaaga
     6541 aattcaaaga gcgagagatg tattttttaa tgtctatggt caggataaat aaattagata
     6601 aaattttaaa agtaatatga aaatatagat attcaatgat attaaggaga tttataatga
     6661 tataataagt ttaatagtta agaagtaaaa tatgaaatgt aatagtatat aaattcattt
     6721 aagactaaaa agaataagat aaaataaaaa ggagtctgta tttctatgta ttacttatta
     6781 aacaaaagca caaatcctta ctttaatttg gcattagaag aatatctatt tttaaatgat
     6841 aaatataatg atgatattat aatcatatgg agaaatgaag aatccatttt tataggtaaa
     6901 aatcaaaatc catatcagga agtatatcat gatgttattg aaaagggaga aattcctatt
     6961 ttaagaagaa tatctggagg aggaactgtt tatcatgact taggtaatat caatatgtca
     7021 ttcatacaaa aagataggca acttcatgag atagattttt tggagcatac taagtttatg
     7081 caaaagatgc tttcaacctt aggactagat gtatctataa ctgaaagaaa agatttattt
     7141 ttaaatggga aaaaaatttc aggttctgca cagtctataa aaagaaaaaa ttctctttat
     7201 catggaactc ttctttacga ttcagattta aataaattaa ctaaatattt aaattcaaat
     7261 aaagcaactg aatcaaatgc aactaaatct gtttcaagta aagttacaaa tattaagttt
     7321 ttactcgaaa aagatataaa ttacttttta gattattgtg ttgaatattt aaaatctaat
     7381 ataaagaata taaaagaact tgaattggat gaagaagata ttaaaagtgt atacaatata
     7441 ggagaagata aatataaaaa gatagagtgg acatatggta aaacgccgaa atttcaagta
     7501 gtgctaccat ttaataatga aagtaaaata aatatacatg ttaacagatg gaaagtatca
     7561 aaatttttta tttcttatga tgataatgta attaatatgg acaagttgtt agatttgaat
     7621 ttctttaaag aagaaataaa agagagtatt ttagaaaatt atccacatta catagattta
     7681 atcaatttaa tattttaata taaaacctct aatttgataa aattgtctta tatcaaacta
     7741 gaggttttta atattcttgc atattaacaa ccattacaa
//
LOCUS       DAELLV010000178         7537 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.178, whole genome shotgun sequence.
ACCESSION   DAELLV010000178 DAELLV010000000
VERSION     DAELLV010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7537
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.178"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            75..2345
                     /locus_tag="KPD58_002850"
     CDS             75..2345
                     /locus_tag="KPD58_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG0266584.1"
                     /translation="MDIIKKLSHGCTLDCHDCCKFNVYTKGNNVVKIEGDKNHPYTKG
                     FICKKGMAHLDRLNHKDRIKTPMLKVDGVWKEISFDKAIEIMAEKLTYYKEKYTSKSV
                     MHYDQYGSGSVLKYIGDIFFNFYGGVSRHKGGPCWSAGMHAQKYDFGVAKSHAIEDML
                     NSKSIFVWGKNPAYTTIHTMQIIKKAKEKGIKIVVIDPIYTKTAQIADKYVQVNPGTD
                     GALAIAMAKIIVEDKLYDEEYINSYVIGFEEYKKYLSSLELSFLIDECGVKENDIREL
                     VDLYTNKYSSINVGYGLQKYKNGGNTIRAIDALGAITGQIGFSGGGVNYANKVYPSVI
                     NSDPYNSQSYGEDREFYVSNISKFIEESLKNTSNKVNYASDELDMALHKVNYISDELD
                     TTSNKVNYVSDELDITSNKVNYVSDELDMTSNKVNYVLDELDITSNKIDYISNELYNL
                     SNKSIKDNIPIKMAVITKSNMLNQLPDLVELERVFSKIEFKVCFDMFITDTATLCDMF
                     IPCTNTLESEDIIYSSMTNPYITYNERAVKPAHKLMDEYYFFMELAKKMGLNDYPFVE
                     KRTYLEKVIEPLKRFDKNLDIEKLKNNYFTIHNPVAWEDKKFETPSGKYELYSESIKN
                     LGISPTPVYISNKYKEIEDKNISFRLLTNHHADTLFSQHFMDKKSIAQAYINRRMAKK
                     VGIEDKDIVILRSKKAKINVQINIDDGVGNYIVKMYVGWWKKHGNPNSLTDTGISDFG
                     GQVTYNESMVEIIRQN"
     gene            2433..2768
                     /gene="spoIIAA"
                     /locus_tag="KPD58_002851"
     CDS             2433..2768
                     /gene="spoIIAA"
                     /locus_tag="KPD58_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HBG0266585.1"
                     /translation="MVNYSLEHKNLYIEFMCSELDHHVANEIREEIDNLLSVNQVKNV
                     VFNFENINFMDSSGIGVIIGRYKKISNEGGRVSVINISSRVKKIFDLSGLNKIIGIYD
                     TYEEALSSL"
     gene            2782..3210
                     /locus_tag="KPD58_002852"
     CDS             2782..3210
                     /locus_tag="KPD58_002852"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HBG0266586.1"
                     /translation="MNNIMEVKFSAISENESLARVIVASFAAKLDPTVDELVDIKTAV
                     SEAVTNAIIHGYEEDSSKFVFLRCEIEGNTIKIIVEDEGYGIENVEKAMEPLYTSKPE
                     LDRSGMGFTVMKSFMDDVEVSSVKDNGTRIEMTKKINVPK"
     gene            3233..3997
                     /gene="sigF"
                     /locus_tag="KPD58_002853"
     CDS             3233..3997
                     /gene="sigF"
                     /locus_tag="KPD58_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HBG0266587.1"
                     /translation="MEVTVAREEKKPLLSHEETLELIEKVQNGDEEAKEILISSNLGL
                     VRSVVSRFLNIGYDREDLFQLGSIGLIKSIYKFDPKFNVKFSTYAVPMILGEIKRYLR
                     DDGMIKVSRSLKQIAVKAKMESEALTKKLGREPSIEELAKAIDVEKEDLVMAMEANFN
                     VEYLQGVIHEEEGSPICLIDKISMKGESEEEKVVDNILLKDILGRLDKRERQIIVLRY
                     FEDMTQSEIGEMLNISQVQVSRIEKKVLSKLKEYIS"
     gene            4277..4714
                     /gene="spoVAC"
                     /locus_tag="KPD58_002854"
     CDS             4277..4714
                     /gene="spoVAC"
                     /locus_tag="KPD58_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HBG0266588.1"
                     /translation="MDKNYKKYVDQISPKPTYLKNYTLAFIVGGIICMIGQAINDLYM
                     KVGGLDKLGASSATSITLIFIGAFLTGLGVYDLIGKRAGAGSIIPITGFANSIVSPAM
                     EYKREGYVLGVGANLFKIAGPVLVYGIGSSILCGIIYYIFKMF"
     gene            4868..5881
                     /gene="spoVAD"
                     /locus_tag="KPD58_002855"
     CDS             4868..5881
                     /gene="spoVAD"
                     /locus_tag="KPD58_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HBG0266589.1"
                     /translation="MKNKRIGKRTVKLENKPTIISTGTIVGPKEGEGPLKDYFDMIMT
                     DDLYGEKTWELAESKMVETASQQAIQKAGKKLSDVNYMLGGDLINQIVPASFAARELA
                     IPFLGIYGACSTMAEGLCIGSMLIDGGFADLVLSGTSSHYCTAERQFRFPLELGNQKP
                     MTAQWTVTGAGSVLLAPNGDGPKVKYVTVGKVIDEGIDDGNNMGPAMAPAAIDTIYSY
                     FEDTNDDPNSFDIIATGDLGTLGKQIAEDFLKEKGVDISKVYTDCGIEMFNLKEQDVH
                     CGGSGCGCSATVFAGYIYDKLRKKEFNKVMLVSTGALLSPTSTLQKQTIPCVAHAVVI
                     VNE"
     gene            5895..6257
                     /gene="spoVAE"
                     /locus_tag="KPD58_002856"
     CDS             5895..6257
                     /gene="spoVAE"
                     /locus_tag="KPD58_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HBG0266590.1"
                     /translation="MLMDYVRVFIVGGIICLIAQVMMDYFKMQTPYVMVTYVTTGVVL
                     TFLGLYEPLVKFAAAGATVPIIGFGYSLATGVIKSIQSDGFLGIFLGGTTATAGGIAA
                     AIFFGYIMSVVFTPKAKP"
     gene            6440..7456
                     /locus_tag="KPD58_002857"
     CDS             6440..7456
                     /locus_tag="KPD58_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG0266591.1"
                     /translation="MVTIKDIARNLGISYSTVSRCLNNNPNVSEKTKKKVVEEANRLG
                     FHFNVNARNLAKKETNRIGVIFSNNFNHQDTRKFFSDIMDSSINSIETNKYDFIIQPN
                     NNISGDSNVYKMVNGQMVDGLVIVSQSIKKEEYDFLKDNNFPHVFIYFKPSFIGEVDN
                     FFWDDNVYGGYIATKHLIDHGHKDIITVTSDDKSSKMHEDRTKGYLNAMEEANLKTEV
                     IKSKMDFESQVEFLEKYIDKIKKASAIFVQQDVPAISIIQELKTTYGINVPEDISIIG
                     YNNIELISYFRSHLSTIDDPREQVIKNGVDSLVNIINKKSTEHIPRKLYPRLIIRNSV
                     KRIK"
BASE COUNT         3034 a          765 c         1398 g         2340 t
ORIGIN      
        1 catagaaagt ttaataaatt atagtatata tgttaatttt taataaggta agaaagtaga
       61 aagaaggata ttttatggat ataataaaaa aattaagtca tgggtgtaca ttagattgtc
      121 atgactgttg taaattcaat gtttatacta aaggaaataa tgtagtaaaa attgaaggag
      181 ataaaaatca tccttataca aaaggtttta tatgcaaaaa agggatggcc catttagata
      241 gattaaatca taaagataga ataaagactc ctatgcttaa agttgatgga gtatggaaag
      301 agatttcttt tgataaagct atagaaataa tggcagaaaa gcttacatac tataaagaaa
      361 agtatacatc aaagtctgtc atgcattatg accaatatgg aagtgggtct gtgctaaaat
      421 acataggaga tatatttttt aatttttatg gaggtgttag tagacataag gggggtccct
      481 gttggagtgc agggatgcat gctcaaaaat atgattttgg agtagctaag agtcatgcta
      541 tagaagatat gttaaatagt aaaagtatat ttgtgtgggg aaaaaatcca gcatatacta
      601 caattcatac tatgcaaata attaaaaaag ctaaagaaaa agggataaag atagtagtga
      661 ttgaccccat atatacaaaa acagctcaaa tagcagataa gtatgtacaa gtaaatcctg
      721 gaacagatgg agctttagct atagctatgg caaaaattat agtagaagat aaattatatg
      781 atgaagaata tataaactcg tatgttattg gttttgaaga atataaaaaa tatttatctt
      841 cattggaatt aagtttttta attgatgaat gtggtgttaa agagaatgat ataagagagt
      901 tagtggattt atatactaac aaatattcaa gtataaatgt tggatatggg cttcaaaaat
      961 ataaaaatgg tggaaacaca ataagagcaa tagatgcttt aggtgctata actggtcaaa
     1021 taggatttag tggtggaggg gtaaactatg ctaataaagt ttatccgagt gttataaatt
     1081 ctgaccctta caatagccaa tcatatggtg aagataggga gttttatgta agtaatatca
     1141 gtaaatttat agaagaatct ttaaaaaata catcaaataa agtaaattat gcatcagatg
     1201 aacttgatat ggcattacat aaagtaaatt acatatcaga tgaacttgat acaacatcaa
     1261 ataaagtaaa ttatgtatca gatgaacttg atataacatc aaataaagta aattatgtat
     1321 cagatgaact tgatatgaca tcaaataaag taaattatgt attagatgaa cttgatataa
     1381 catcaaataa aatagattat atatcaaatg aattgtataa tttatctaat aagtcaatca
     1441 aagataatat tcctataaag atggctgtga taactaaaag taatatgctg aatcaactgc
     1501 cagatttagt agaattggag agggtgtttt caaaaattga atttaaagta tgttttgata
     1561 tgtttataac tgatacagct actttatgtg acatgtttat accatgtaca aatacactag
     1621 agagtgaaga tataatttat agttcaatga ccaatccata tataacctat aatgaaagag
     1681 ctgtaaagcc agcacataaa ttgatggatg aatattactt ctttatggag ctagctaaaa
     1741 agatgggatt aaatgattat ccttttgtag aaaagagaac ttatttagaa aaagtgattg
     1801 aaccacttaa gcgttttgac aaaaatctgg atatagaaaa acttaaaaac aattacttta
     1861 ctatacataa ccctgttgct tgggaagata agaagtttga gactccatct ggaaaatacg
     1921 agctttattc agaaagtata aaaaacttgg gaataagtcc aacacctgta tatataagca
     1981 ataaatataa agaaatagaa gataaaaata tttcttttag attattaact aatcatcatg
     2041 cagatacttt atttagtcag cattttatgg acaaaaaatc tatagctcaa gcatatataa
     2101 atcggagaat ggctaaaaaa gtaggtattg aggacaaaga tattgtgata ttgaggtcaa
     2161 aaaaagcaaa aataaatgta caaataaata tagatgatgg agtaggtaat tatatagtaa
     2221 aaatgtatgt tggttggtgg aagaaacatg gaaatcctaa tagtttaaca gacactggta
     2281 tatcagattt tgggggtcag gtaacatata atgaaagtat ggtagaaata atacgacaaa
     2341 actagttaaa tttcattgaa ggaataaaaa tataattata gaattgatta aaagttacta
     2401 tttttacaaa actattgaag gagggatttt tgatggtaaa ttattcctta gaacataaga
     2461 atctatacat agaatttatg tgctcagaac tagaccacca tgtagccaat gaaataaggg
     2521 aagaaataga taacttatta agtgtaaatc aagtaaaaaa tgttgtattt aattttgaaa
     2581 atattaattt tatggattct tcaggtatag gcgtcatcat aggtagatat aaaaagattt
     2641 ctaatgaagg gggacgagtt tcagtaatta atataagttc acgagttaag aagatttttg
     2701 atttatctgg gttaaataaa attattggca tttatgatac gtatgaagaa gctctaagtt
     2761 ctttgtaggg aggagtatgt gatgaataat attatggaag ttaaattttc ggcaatatct
     2821 gaaaatgaaa gtttagcaag agttatagta gcatcctttg cagctaagtt agacccaaca
     2881 gtagatgagt tagtagatat aaaaactgct gtctctgagg ctgttacgaa tgctataata
     2941 catggatatg aagaagacag ttctaagttt gttttcttaa gatgtgagat agaaggaaat
     3001 actataaaga ttatagttga agatgaaggg tatggaatag aaaatgtgga gaaggcaatg
     3061 gaaccacttt atacatcaaa accagaactt gatagatcag gaatgggatt cacagtaatg
     3121 aaaagcttta tggacgatgt agaggtaagt tctgtaaaag ataatgggac tagaattgaa
     3181 atgactaaaa aaataaatgt gcctaaataa agtccaaaag tgaggtttta ttatggaagt
     3241 aactgttgcc agagaagaaa aaaaacctct tcttagtcat gaagagactc tagaacttat
     3301 tgaaaaagtt caaaatggag atgaagaggc aaaagaaata ttaatatcaa gcaatttagg
     3361 tctagttagg agcgttgtat ctagattttt aaatatagga tatgatagag aagatttatt
     3421 tcagttaggt tctataggct taataaaatc aatatataaa tttgacccaa aatttaatgt
     3481 aaaattctct acttatgcag taccaatgat tttaggtgaa ataaagagat acttaagaga
     3541 tgatggtatg ataaaagtat caaggtcatt aaagcaaata gctgtaaagg caaagatgga
     3601 aagtgaagct ttaactaaaa aattaggtag agagccgagt atagaagaat tagctaaagc
     3661 aatagatgta gaaaaagaag atttagtaat ggctatggaa gctaatttca atgttgagta
     3721 tttgcaagga gttattcatg aagaggaagg ttctccaata tgccttattg ataaaataag
     3781 catgaaagga gaaagtgaag aagaaaaagt agtggataat atattgttaa aagacatact
     3841 tggaagactg gataagagag agcgtcaaat aatagttctc agatactttg aagacatgac
     3901 tcaaagtgaa ataggagaga tgctcaatat atcacaagtt caagtatcta ggatagagaa
     3961 gaaagtattg tctaagttaa aagaatatat atcgtgataa tacttatgat atgtagaata
     4021 acaaaatata ataaatatat tactaaaata aaatgtaata actttttatt ttaagttttt
     4081 aaattattaa aagatattaa aataataaaa agcattaaaa aagtaaaaat ataaaaacaa
     4141 ggtgtcttta tggaaaaata gattttttct ttattaggca tcttttttat tttttgacca
     4201 aactttagat agtcaaaata aataataggt ttattttttt aaaattaaca aataatactt
     4261 tagagaggtg tttagtatgg ataaaaatta taaaaaatat gtagaccaaa taagcccaaa
     4321 acctacatat ttaaaaaact atacacttgc atttattgta ggtggaatta tttgtatgat
     4381 agggcaagca ataaatgatt tgtatatgaa agttggaggt cttgataaac taggtgctag
     4441 ttctgcaaca tcaataacat taatatttat aggggcattt ttaactggat taggagtcta
     4501 cgacctaata ggaaaaagag ctggagctgg ttctataatt ccaataacag gttttgctaa
     4561 ctcaatagta tctccagcaa tggagtataa aagagaaggc tatgtattag gtgtaggtgc
     4621 aaacttattt aaaattgcag gaccagtttt agtttatggt ataggttctt ctattttatg
     4681 tggaattatt tattatattt ttaagatgtt ttagatgatt tttatttaat ttaaatttta
     4741 ttaattatga agaaggtata aaatctgaaa atagctacta aagtaactaa ttgaaaaata
     4801 attgtgcaga tttaattaat attaaaaatt tgaatagtaa aatgcaaaat taaaaaggtg
     4861 tgaaaatatg aaaaataaaa gaattggaaa aagaacagtc aagttagaaa ataaaccaac
     4921 cataatatca actggaacta tagtaggtcc taaggaaggt gaaggaccac taaaggacta
     4981 ttttgatatg attatgactg acgatttata tggtgaaaag acttgggaat tagctgaaag
     5041 taaaatggta gagactgcat ctcaacaagc tatccaaaag gcaggtaaaa aattgtcaga
     5101 tgtaaattat atgctaggtg gcgatttaat aaatcaaata gttccagcat catttgcagc
     5161 aagagaactg gctattccat ttttaggtat atatggagct tgttctacaa tggcagaagg
     5221 tctttgtata ggttctatgc ttatagatgg tggttttgct gatttagtac tgtcaggtac
     5281 atcaagtcac tattgtacag cagaaagaca atttagattc ccacttgaac ttggaaatca
     5341 aaaaccaatg acagctcagt ggacagttac aggagcagga agtgtattat tagctcctaa
     5401 tggagacggt ccaaaagtaa agtatgttac tgttggtaaa gtaatagatg agggtattga
     5461 tgatggtaat aatatgggac cagctatggc tccagcagca atagatacta tctactctta
     5521 ctttgaagat actaatgatg acccaaatag ctttgatata attgctactg gagatttagg
     5581 tactcttgga aaacagatag cagaagactt tctaaaagaa aagggtgtag atatatcaaa
     5641 agtttatact gactgtggta tagagatgtt taaccttaaa gaacaagatg tccactgtgg
     5701 tggaagtgga tgtgggtgtt cagctacagt gtttgcagga tatatatatg ataagttgag
     5761 aaaaaaagaa tttaacaaag taatgcttgt atcaacagga gcactacttt caccgacaag
     5821 tacactacaa aagcagacaa taccatgtgt tgcacatgca gtagtaatag taaatgagta
     5881 ggaagtgata taatatgtta atggactatg taagagtatt tattgtaggt ggaattatat
     5941 gtttaatagc ccaagtaatg atggattatt tcaaaatgca aactccatat gttatggtaa
     6001 cgtatgtaac aactggtgta gttttaactt ttctaggact ttatgagcca ttagttaagt
     6061 ttgcagcagc aggtgcaaca gttcctataa taggatttgg atattcactt gctacaggag
     6121 taataaaatc aattcaaagt gatggttttt taggtatttt tttaggaggt acaactgcta
     6181 cagctggtgg aatagcagca gctatattct ttggatatat tatgtcagta gtatttactc
     6241 caaaagcaaa accataaatc tttattaaaa gaataataag tttttgaaat aataactttt
     6301 tataaagcag gtggatatct tttagattcc acctattttt tatataaaga agcgtttaca
     6361 taattgaagt tgacaattat ttacaacata gtgtagtatt atattatcaa aataaaatga
     6421 agtgaagtag gggataaaga tggtaactat taaagatatt gcaaggaatc taggtatcag
     6481 ttattcaact gtttctaggt gcttaaacaa taatcctaat gtatctgaaa aaacaaaaaa
     6541 gaaagtggta gaagaggcaa atagactagg gttccatttt aatgtaaatg caagaaattt
     6601 agcaaaaaaa gaaactaaca ggataggggt aatcttttca aataatttta atcatcaaga
     6661 tactagaaag ttttttagtg atataatgga tagttctata aattctattg agactaataa
     6721 atacgatttt ataatacagc ctaataataa cattagtggt gatagtaatg tatataaaat
     6781 ggtaaatgga caaatggttg acggattagt aattgtgtct caatcaataa aaaaagaaga
     6841 gtatgatttt ttaaaagaca ataattttcc acatgtattt atatatttta aaccatcttt
     6901 tataggagaa gtagataatt tcttttggga cgataacgtg tatggaggtt atatagctac
     6961 caagcattta attgaccatg gtcataaaga tataataaca gtaacttcag atgataagag
     7021 ttcaaaaatg catgaagata gaaccaaggg atatttgaat gctatggaag aagcaaattt
     7081 aaagacagaa gtcattaaat ctaaaatgga ttttgaatct caagttgaat ttttagaaaa
     7141 atacattgat aaaataaaaa aagcttcagc tatatttgta caacaagatg taccagcgat
     7201 ttctataata caagagttaa aaactactta tggtataaat gtaccagaag atatttccat
     7261 aatagggtat aataatattg aattaatatc atattttagg tcgcatttat caacaataga
     7321 tgacccaaga gagcaagtta taaaaaatgg agtagatagt ttagtaaata taataaataa
     7381 aaagagtaca gaacacattc cgagaaaatt atatccaagg ttaataatac gaaatagcgt
     7441 taaaagaata aagtagtaag cttttaagta tcaaacgttt attaaaaata ttatagatta
     7501 tgtgaagttt tttaacaaat aatataaata aaaatta
//
LOCUS       DAELLV010000179         7534 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.179, whole genome shotgun sequence.
ACCESSION   DAELLV010000179 DAELLV010000000
VERSION     DAELLV010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7534
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.179"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(441..1691)
                     /gene="cme"
                     /locus_tag="KPD58_002858"
     CDS             complement(441..1691)
                     /gene="cme"
                     /locus_tag="KPD58_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Cme"
                     /protein_id="HBG0266592.1"
                     /translation="MENKNTNSTWKKKVILFLASQYISLFGSSLVQMAMIWYVTLQTS
                     SGVWVTVLTVCSFIPQMIISFFAGVWADYYNRKVLIIVSDSTIAISTLVLILFMMSSN
                     GTTSELIAISVISVIRSLGSGVQTPAVNAMIPQLVPEEHLMRFNGINSSLQSIVQFIV
                     PIVAAAILGFGNKINQVLLIDVFTAIIAIVLLIFIKIPKHDIPKQTEKVSFFADMVSG
                     IKYCFSDKFIRKVLFTYGAFMLLCVPSGFLIGLLIERTFGKGYIYLSIIEMIGFLGMV
                     LGGLIIGVFGGFKNRNKTFFLAILSYAIFSIILGLVTQAWQFFIFMFFTNFSIPIIQA
                     SAMTMLQEHVAPQMLGRVLSLPIIIYTGFIPLGMMIFGPMADVISINNLIIIAGIILL
                     FFALAIPYSKQFYKQGISKSNESE"
     gene            complement(1777..2523)
                     /locus_tag="KPD58_002859"
     CDS             complement(1777..2523)
                     /locus_tag="KPD58_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0266593.1"
                     /translation="MDTYTTSEVADLIGVHPNTVRLYEELNLIPKPKRNTNGYRIFND
                     FHIEQFRFARTALKIEVVQRGLRKDALEIVRTSANRDFDKAISLVNQYLQQLEIEKSN
                     AEEALTIVAQILNGNTPETNKLFLTRKETAEHLNVTIDTLRNWEMNGLLKVKRKQNGY
                     RVYTDEDIKRLKIISALRCANYSLSAILRMLNALSSSPNTNIKTVLDTPKDDDEIISV
                     CDKLITSLKEAEHNAKTMKSHLQIMKKQFS"
     gene            complement(2676..3698)
                     /locus_tag="KPD58_002860"
     CDS             complement(2676..3698)
                     /locus_tag="KPD58_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HBG0266594.1"
                     /translation="MNKNNCLDLIFEKVDPSSVGMSSEKLSQLEPLIISQYSNINGII
                     IARKGKIVFEKYYNGYNADDTFHVASVTKSIISALIGIAIDKGYIKDVDQKVVDFFPE
                     YTCSPSEICKKAVTIRHLLTMTAPYPFKNMTESLERLCKQKDWIKYTLNQLGQKGEIG
                     TFKYSSAGTHLLSAIITKTTGKSAREFANEYLFKPIGMKVIPDYDMKDFGFEDFFGKN
                     LKGWAKDRQGYSIGGWGLTLSARDMLRFGFLYLNDGIWNNKRIISKSWIEESVKMNSN
                     EYGYLWWLCKKDGVLWYSAMGTGGNAICCIPQYDLVIAIPSKIVFKPRDRWEFIEKYI
                     LTSIID"
     gene            complement(3698..6118)
                     /locus_tag="KPD58_002861"
     CDS             complement(3698..6118)
                     /locus_tag="KPD58_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG0266595.1"
                     /translation="MKEIKINGAKIHNLKNIDVSIPKDKLVVATGVSGSGKSSLMFDI
                     VFEEGRKQYLQSLGILAGIDSEDKFDNIQGLAPTIAVQQNIIRQSNPRSTVGTRTNIL
                     NMLTLLYSVEGQIMCTMCDTPVSDNLICNNCGYEEERLRPSYFSYNSSDGMCMKCSGH
                     GAYFEINIEKLVLDKHDTLKQVLDRAKITPGYLRVFSKKFNDYLDIQFLQLPEEIRNE
                     VLYGHYENGKKSSSLSKVFHNRYKRGEDLNGVYTMTACSDCNGFKIGEEARRVLLNGK
                     HIGELGKMTILEIDDFLKLLINQGNLNTFGTNLVNDILSKTQHLIESRLGHLSLYREM
                     STLSGGEMQRLFLNSHLDSEMDSLIYVLDEPTAGLHESEKIDILKSLKKLKDLGNTVI
                     VVEHDKNTIEMAEHIIDIGPKAGVEGGQLVYQGDLEGLLQSDKSLTGQYLSGKYPMPN
                     RTSNKNITHTDKIPCITIQNANTNNLKNVTVSLPLGAMVGISGKSGSGKSSLISDTLL
                     PLLRSQFNNHTINNQINSSESEIGEEDDYAVVETIADRLIGTKHISGYSEISQSPIGK
                     NMNSTPASYIGIWDKIRTLFAGQPESLEQGFTAGHFSFNSKGACPKCSGSGYEKVWLG
                     NNLSIDHICSECQGKRFNDESLSIKYKNKNIHDILNMSVSESVHFFKDIPNIVSHLNV
                     LEQIGMGYIKLGQPTPTLSGGESQRIKLAKEIGKKRNGNILYVLDEPTTGLSLYDTSK
                     LIQLLDELVENGNSVIVVEHDIDVLNVCDWIVELGPEGGDKGGYIIAEGSPKTLKENP
                     KSITGRYL"
     gene            complement(6204..7124)
                     /locus_tag="KPD58_002862"
     CDS             complement(6204..7124)
                     /locus_tag="KPD58_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG0266596.1"
                     /translation="MTNAKSKKEYLNRICKVQDYIEEHLHEPLSLDELSNIAGFSKFH
                     FHRIFKAIEKETLAQYVNRLKLENATAFLIHRTDMTVTDIAHYFGFTDSAVFSRAFKN
                     YYKISPIKYRNNYSKNCKEPYKISQYNRSISKPKCKNTREVKGEIEILELDNMNTIYT
                     RYTGSYGNLTSTFPKLLERLFTYASEQNLLGLENTQILAIYHDNPQFTQEHQLRTSIC
                     MTIPDNIVVKENDNLGNMIIPSGKYLVGHFDISKSEYSDAWDFMYNEWLSNGNYKLRA
                     SFPFEVYLNNPHNHPQNRHLVDIYLPIEPF"
BASE COUNT         2524 a         1185 c         1076 g         2749 t
ORIGIN      
        1 ttgtatactt taatttatgt agtgtaatag ttagttttat tttataatta ttcaatataa
       61 tttagtaaga agttcagtac aattagagct acttagtgga aaacttaata ggtacttaaa
      121 ctatataaat actttatttt cctccatttg tactataaat ttaatttatg atataaatgg
      181 aggtattatt atatgatttg ataaatttca tcattataac ctacaagtaa aggtattatt
      241 atataaagtt aataaagata gtatcaaaat attatttgct ataaagagag ggactcaaaa
      301 taaagataaa cctatttttc aatgttgaaa tgataaaata tatttgattt catataaaaa
      361 attttcttca taaattaaaa agtccatttt aagccccttc atttttaacc agataaatcg
      421 agttaaacta tttaaatata ttactcggat tcatttgact tagatattcc ttgcttgtaa
      481 aattgttttg aataggggat agccaaagca aaaaataaaa gtataatccc tgctatgatt
      541 attaaattat taatagaaat tacatctgcc attggtccga aaatcatcat accaagagga
      601 ataaaaccag tatatataat aataggtaag ctaagaactc tacctagcat ttgtggtgct
      661 acatgctctt gcaacatagt catagctgat gcttgaatga ttggaattga aaaattcgta
      721 aaaaacataa atataaaaaa ttgccaagct tgtgttacaa gacctagaat aattgaaaaa
      781 atagcatagg ataatattgc tagaaaaaag gtcttatttc tatttttaaa accaccaaaa
      841 actcctataa tcaatccacc taaaaccata cctaaaaatc caatcatttc aataatagac
      901 aggtatatat aacccttgcc aaatgttcgt tcaataagaa gtcctataag aaatcctgat
      961 ggaacacaaa gaagcatgaa tgctccataa gtaaaaagca cttttctaat gaacttatct
     1021 gaaaaacagt actttatccc tgataccata tctgcaaaaa atgagacttt ttcagtttgt
     1081 tttggtatat catgtttagg aattttgatg aatattaata atacaatagc aataattgca
     1141 gtaaatacat caattaaaag aacttgattt attttattac caaatccaag tattgcagca
     1201 gctacaatag gaactataaa ttgtactata gactgtagac tactatttat accattaaaa
     1261 cgcattaaat gttcttctgg tacaagttga ggaatcattg cgtttacagc aggtgtttgt
     1321 acaccacttc ctagtgaacg tataacagat attactgata tggctattaa ttcacttgtt
     1381 gtgccatttg atgacatcat aaatagaatt agaactaatg tagatatagc aattgttgaa
     1441 tctgatacaa ttatgagtac ctttcgattg tagtagtctg cccatacacc agcaaaaaaa
     1501 gaaattatca tttgaggtat aaaggagcat acagttaaaa ctgttaccca tacacctgat
     1561 gatgtctgaa gtgtgacata ccatatcatt gccatttgta caagtgatga gccaaataag
     1621 gaaatatatt gacttgctaa aaaaagaatg acttttttct tccatgtaga attagtattt
     1681 ttgttttcca tcctattcct cctttgattt gtgtgttgaa tttttcttgc tcttaaggat
     1741 aacacacaaa tcaaagtctg gagttagagt ggagggctag gaaaattgtt ttttcataat
     1801 ttgaagatga gatttcatgg tttttgcatt atgttcagct tctttcaatg aagttatcag
     1861 tttgtcacac actgagataa tttcgtcatc atcttttgga gtgtcaagga cagttttaat
     1921 attagtatta ggactagaag ataaagcatt taacattcgt aaaatagctg aaagagaata
     1981 gtttgcacaa cgaagtgcgc ttataatttt aagtcgtttt atatcttcat cagtatatac
     2041 acgataacca ttttgttttc tttttacttt taacaaacca ttcatttccc aatttcttaa
     2101 agtatctatt gtaacgttta agtgttctgc agtttctttt ctagttaaaa agagtttatt
     2161 tgtttctgga gtatttccat ttaaaatctg tgcaacaata gtaagtgctt cctcggcatt
     2221 agatttttct atttctaatt gttgcaagta ttgatttaca agactaatag ccttatcaaa
     2281 gtccctattc gctgatgttc ttacaatctc aagggcatct tttcttaatc ccctttgtac
     2341 cacttcaatt ttcaaggctg tacgagcaaa tctaaattgt tctatatgaa aatcattaaa
     2401 gatacgataa ccattggtat tacgcttagg tttaggtatt aagtttagtt cttcatataa
     2461 acgtactgta ttaggatgta caccaattaa atctgctacc tctgatgtcg tgtaagtgtc
     2521 cattagtatt caactccttt tagatatata gtattagtca taataacacc aaacaaaagt
     2581 ctggtgttat aatggagggt ttggctatct aagagtaatt agtttatgaa agagtaatta
     2641 gtttatgaaa gagtaattag tttatgaaag agtaattaat ctattataga agtaagtata
     2701 tatttttcaa tgaactccca tctatcacga ggtttaaaca ctattttaga tggtatggct
     2761 attactaagt catattgtgg aatacaacaa atagcatttc ctccagttcc cattgctgaa
     2821 taccacaaaa caccatcttt tttacatagc caccacagat aaccgtattc attactattc
     2881 attttaactg attcttcaat ccacgattta gatataatac gtttattatt ccaaatacca
     2941 tcatttaaat acagaaaacc aaatcgcaac atatctctag cactcaatgt aagtccccag
     3001 ccacctatag aataaccctg tctatctttt gcccaaccct taagattttt tccaaagaaa
     3061 tcttcaaaac caaaatcttt catatcataa tctggtataa ctttcatacc aataggttta
     3121 aataagtact cattagcaaa ttcacgagca gattttccag tagtttttgt tataatagct
     3181 gaaagtaggt gcgttccagc tgaagaatat ttgaaagtac caatttcgcc tttttgacca
     3241 agttgattca aggtatattt tatccaatct ttttgtttgc aaagtctttc taaagactca
     3301 gtcatatttt taaaagggta tggtgcagtc attgtaagta ggtgacgtat agttactgct
     3361 tttttacata tttcagaagg actacaagta tattcaggaa aaaaatcaac aaccttttgg
     3421 tcaacatctt ttatatatcc tttatctatt gcaataccta ttaaggctga tataatactt
     3481 tttgtaactg atgcaacatg aaaagtatca tcagcattat atccattata gtacttttca
     3541 aagacaattt ttccttttct tgcaataatt ataccattta tattgctata ttgtgagatt
     3601 attaaaggtt caagttgtga aagcttttct gaactcatac caacacttga agggtctact
     3661 ttttcaaaaa ttaagtctaa gcaattgttt ttattcatta aagatacctc cctgttatag
     3721 attttggatt ttcttttaga gtttttggag aaccttctgc aattatataa cctcctttgt
     3781 caccaccttc tggaccaagc tcaacaatcc agtcacaaac atttaataca tctatatcat
     3841 gttcaacaac aattacagaa tttccatttt caaccaattc atctaataat tgaatcaact
     3901 tagaagtatc atatagactt agaccagtag ttggctcatc caatacataa aggatattac
     3961 catttcgttt tttaccaatt tcttttgcaa gtttgattct ttgggattca ccaccactta
     4021 aagtcggtgt aggttggcct aattttatat accccatacc tatttgttct aatacattta
     4081 agtgtgaaac aatatttgga atatctttga agaaatgtac agattcagaa acactcatat
     4141 ttaaaatatc atgtatattt ttatttttat atttaataga taaagattca tcattaaatc
     4201 tttttccctg acattcactg catatatggt ctatacttag attatttccc aaccagacct
     4261 tttcatatcc actaccacta catttaggac aagccccttt ggagttaaat gagaaatgac
     4321 cagctgtaaa tccttgttct aacgattctg gctgacctgc aaatagtgta cgtatcttat
     4381 cccatatacc aatataagaa gcaggagttg agttcatatt tttgcctata ggagattgag
     4441 atatctctga atatccagaa atatgtttag taccaatcag tctatctgca atagtttcaa
     4501 caactgcata atcgtcttcc tcacctattt cactctctga agaatttatt tgattattga
     4561 tagtatggtt attaaattgg cttctaagaa gaggaagtag ggtgtctgat ataagtgaac
     4621 tcttgccact acctgatttt ccagatattc caaccatagc acccaaaggt aatgatacag
     4681 ttacattttt aagattattt gtatttgcat tttgaatagt tatacaaggt attttatcag
     4741 tatgtgtaat gtttttattg cttgttctat taggcattgg atattttcca gatagatact
     4801 gtccagtaag tgatttgtcg gattgaagta gaccttctaa atctccttga tatactaatt
     4861 gaccaccttc aacaccagct tttggaccaa tatcaataat atgttctgcc atttcaattg
     4921 tgtttttatc atgctcaact actatgaccg tattgcctaa atctttaagt ttctttagtg
     4981 attttaagat gtctattttt tctgattcat gcaatccagc tgttggttca tcaagcacat
     5041 aaattaatga gtccatctct gaatcgagat gtgaatttaa aaatagtctt tgcatttccc
     5101 caccactcaa agttgacatt tctcggtata aggatagatg acctaatcgt gattctatta
     5161 aatgttgagt tttggataaa atatcattta caagatttgt accaaatgtg tttaaattac
     5221 cttgatttat taagagttta aggaaatcat caatttctaa aatcgtcatt tttccaagtt
     5281 ctccaatatg ttttccgttt aaaagtactc tacgtgcttc ttcaccaatt ttaaaaccat
     5341 tgcaatctga acaggcagtc atagtataaa ctccatttaa atcttcgccc cttttgtaac
     5401 gattgtgaaa tacctttgat aaagaggaac tttttttacc attttcataa tgaccataga
     5461 gtacttcatt tcttatttct tcaggtagtt gtaagaattg tatgtctaaa taatcattga
     5521 attttttaga aaatactctc aaatatccag gtgtaatctt tgctctatct aagacttgtt
     5581 ttagggtatc atgcttatca aggactaatt tttcaatatt aatctcaaaa tatgcaccat
     5641 gtccagagca cttcatacac attccatctg aagagttgta tgagaaataa cttggtctta
     5701 atcgttcttc ctcatatcca caattattgc aaattagatt atcgcttaca ggagtatcac
     5761 acatagtaca cattatttga ccttccactg aatacaatag tgtaagcatg ttgagaatat
     5821 tggttcttgt gcctactgtt gaacgaggat tgctctggcg aataatattt tgttgaacag
     5881 ctatagtggg agctaaacct tgaatattat caaacttatc ttcactatca atacctgcaa
     5941 gtatacctaa agattgcaga tattgtttac gcccttcctc aaatacaatg tcaaacataa
     6001 ggctagactt acctgagcca ctgacacctg ttgcaacaac aagcttatct tttggaatag
     6061 aaacatctat atttttaaga ttgtgtatct ttgctccatt aatttttatt tctttcattg
     6121 gttcacctca ttttatcaat tttatcaaac cgtaaaatag ggatatatta atccttaata
     6181 caagaataga ttaggtgagg ttattaaaaa ggttctatag gtaaatatat atcgaccaag
     6241 tgtctatttt gagggtgatt gtgtgggttg ttaagatata cttcaaaagg gaaggaggca
     6301 cgtagcttat aattaccatt tgatagccat tcattataca taaaatccca cgcatcacta
     6361 tattcagatt ttgaaatatc aaaatgtccc acaagatatt ttccagaagg tattatcata
     6421 ttgccaagat tgtcattctc tttgacaaca atgttatctg gaatggtcat gcaaatacta
     6481 gtccttaatt gatgctcctg tgtaaattgt ggattatcat gataaatggc taatatttgt
     6541 gtgttttcaa gtccaagtag attctgctca cttgcatatg tgaataaccg ttctaaaagt
     6601 tttgggaaag tactggttag attaccatat gaaccagtat atctagtgta aatagtattc
     6661 atattatcaa gttctagaat ttcaatttca cctttaacct ctctagtatt cttacattta
     6721 ggttttgata tgcttctatt gtactgagaa attttatatg gttctttgca attcttgcta
     6781 taattgtttc tatattttat agggcttatt ttatagtaat ttttaaatgc tctcgagaaa
     6841 actgctgaat cagtaaagcc gaagtagtgt gcaatatcag taacagtcat gtcagttcta
     6901 tgaataagaa aagcagttgc attttctagt tttagcctat ttacatattg tgccaaagtt
     6961 tctttttcta ttgctttgaa gattctatga aaatgaaatt ttgaaaaacc tgctatatta
     7021 gaaagttcat caagagaaag aggttcatgt aaatgttctt ctatatagtc ttgcactttg
     7081 caaatacgat ttagatattc ttttttactt ttagcatttg tcatatatag ccctccttat
     7141 tgtgtaaaat gtgtatcaat taatttatag caatatagta ataaaatttt atgtttttgt
     7201 aatttatgat aaataagata gcgtttaatt ttttataatt gataaaagat ataagatgcc
     7261 ttatatttgt ctttgcatat aagtatagtc ttcgtaataa ttagtatacc atagtgattt
     7321 atttttaatt gtgtatttat ataaatacac aacttatgat atggacgaat tattatgcaa
     7381 ataagctgta attaaataaa ttgcaatata aaccagttga aacataagtt agggaaatat
     7441 tatcaaagtt attacttagc ataagagtat agatggaata aaataagatt aaaaaattac
     7501 atactaaatt taataaaatt tttatattaa aaat
//
LOCUS       DAELLV010000180         7446 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.180, whole genome shotgun sequence.
ACCESSION   DAELLV010000180 DAELLV010000000
VERSION     DAELLV010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7446
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.180"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            59..838
                     /locus_tag="KPD58_002863"
     CDS             59..838
                     /locus_tag="KPD58_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266597.1"
                     /translation="MKVNENNINNIDEELGELSRKVYLESPDFWISISDKIIDKVFDE
                     MLLFFAVKYNYFNIVCFAIENNYLDLNMPSKNKKYTNIMEHLLDTSKQNEDKKIYNYL
                     LSLNKGNNLDERPILDELNCNISSDTFEKKDIYLPKYLCPSCNSNIFDTGYKVSNDIT
                     YKFSSKEAEPIEMCSEVSSIRCCNCDELLEDITLDKLYNLCKIQNCNNCGSNLTKVGI
                     IAKKKMNFDENTEKFKDVSTSYCCGNCDSEISLSQREHFKI"
     gene            complement(1293..1721)
                     /locus_tag="KPD58_002864"
     CDS             complement(1293..1721)
                     /locus_tag="KPD58_002864"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBG0266598.1"
                     /translation="MIKKLNNKDINKIMEIWEKSTIKAHDFISKEYWQNNYNTVKNEY
                     IPISDTFVYDDGDEIKGFISIIDKSFIGALFIKPKYQNLGIGGKLLDYATKKYKSLSL
                     AVYKDNKKAVVFYNKKGFNIVKEQVNEDSGFKEYIMEYSK"
     repeat_region   1910..3711
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1976..2004
                     /rpt_unit_seq="atttacataccacttagttaatataaaac"
     gene            complement(3895..4161)
                     /gene="cas2"
                     /locus_tag="KPD58_002865"
     CDS             complement(3895..4161)
                     /gene="cas2"
                     /locus_tag="KPD58_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HBG0266599.1"
                     /translation="MFVIVTYDIVEARSLNRIRRILRKYLTWTQNSVFEGNITEGKLH
                     KCISEIENIIDNSEDSIYVYEIKNPNSIKKKCYGIDKYSDEMFI"
     gene            complement(4164..5144)
                     /gene="cas1b"
                     /locus_tag="KPD58_002866"
     CDS             complement(4164..5144)
                     /gene="cas1b"
                     /locus_tag="KPD58_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1"
                     /protein_id="HBG0266600.1"
                     /translation="MYKDYYIISNGDIKRKENTIYYYNLEGEKKILPVEQVDDIHIYG
                     EVDLNTKLINYISKYGVMLHFYNYYGYYSGTYYPRKKNVSGFSLVCQSACYLDKNERL
                     YLAKSFVESASYHILRNMRYYKVDESLIDTIKDELNDLNDVKEISELMGKEGRIRKTY
                     YKSFNQILKDDFEFYKREKRPPKDPVNALMSFGNSIMYTNVLSEIYKTQLDPTISFLH
                     EPSTKRFSLSLDIAEIFKPIIVDTIIFSLINNKRINKNDFEYQDKICYLSEKGKKKFL
                     QEFENKMQTTIKHRNLNRKVSYRMFIRLECYKLIKHFINDERYKVLKVWW"
     gene            complement(5147..5698)
                     /gene="cas4"
                     /locus_tag="KPD58_002867"
     CDS             complement(5147..5698)
                     /gene="cas4"
                     /locus_tag="KPD58_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HBG0266601.1"
                     /translation="MWLKFTKEGDFVPIDLEVLKVQGVKINYYYVCKRKLWLFSKGIT
                     MEQNSSRVLSGKIVHEDSYKRMKNKEILIDDILKLDIIKDEYVKEVKISSKMKEADEM
                     QLYYYLYYLKNSMGIEKKGTINYVKEKKQEELILTEEKERIIENTLININEINMQLSP
                     SKIEKKHYCTKCAYYEFCFVKEE"
     gene            complement(5713..>7446)
                     /gene="cas3"
                     /locus_tag="KPD58_002868"
     CDS             complement(5713..>7446)
                     /gene="cas3"
                     /locus_tag="KPD58_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HBG0266602.1"
                     /translation="ILHRLDHSASAHEVVECNNVINIGEQTENYLMKEFGSLREAQSF
                     AKSNRNKNIILIASTGMGKTETALIWIDKDKAFFTLPLRVSINALFDRAKNIVGVGES
                     NDTFLGLLHSTAIDYLEESNQENSSEIVDLAKLLSCKLTFSTIDQIFKFPFLYRGYEK
                     VYSTLAYSKVVIDEIQAYSPEIAAVLVKGIEMIHKIGGRFMIMTATMPTIYIDELKKR
                     GVMNSNLAELTCNTEKIRHCVSIVENSIDENLGKIIQSGMNKKVLVIVNTVKSAVEKY
                     ELIEEIVKPKGIDVNLNLLHSMYIQEDRAKLEKYIKEFANSDRNGIWITTQLVEASLD
                     IDFDELHTENSTLDSLFQRFGRCYRSREYEENSPNIFIYTEKATGIGSIYDKDIVEKG
                     LELLKTFINGKECVKMKEKYKVEMVKILYSKESLEGTAFEKRFTSALNILDTITPYSL
                     GSKDAQNILRNIEGYTVIPEDIYNKIEETLIKDYEELGQELINAYSNDDRQLINKIKS
                     KRKKARREIIKKTVNLPIYKAKQNVIDIQVKGLEDLKILLYKYDIYENKHTKQIFGKG
                     VLISNEIDQFI"
BASE COUNT         2518 a         1148 c          769 g         3011 t
ORIGIN      
        1 tatataaaaa atagtaacaa atagtttata cacaagatat ttaaaaggaa gtgatgatat
       61 gaaagttaat gaaaataata taaataatat agatgaagag ttaggtgaac ttagtaggaa
      121 agtttactta gagtccccag atttttggat aagtatttct gacaaaataa tagataaagt
      181 atttgatgaa atgttattat tttttgcagt taaatataac tatttcaata tagtatgctt
      241 tgctattgaa aacaattatc tggatttaaa catgccttca aaaaacaaaa aatacactaa
      301 tataatggag catttgttag atacatcaaa gcagaatgaa gataaaaaaa tttataatta
      361 tcttctaagc ttaaataaag gtaataacct agatgaaaga ccaattttag atgaattaaa
      421 ctgtaatatt agtagtgata cttttgaaaa aaaagatata tatcttccaa agtacttatg
      481 tccaagttgt aattctaata tatttgatac aggttataaa gtatcaaatg acataacata
      541 taaattttct tctaaagaag ctgaacctat tgaaatgtgt agtgaagttt cttctatcag
      601 atgttgtaat tgtgatgaat tattagaaga tattacactt gataaactat ataacctatg
      661 caaaattcaa aactgtaata attgtggttc taatttaact aaggtaggaa ttattgcaaa
      721 gaaaaaaatg aactttgatg agaatactga aaaatttaaa gatgtctcaa catcttactg
      781 ttgtggaaat tgtgactctg aaataagctt atctcaaaga gaacatttta agatttaaat
      841 atttataggc taaagtaata agattacatt attagatact gtataagttt tttaaatctg
      901 taaaaataaa tacacttata agaattaaaa ttgctatgaa actagttctt gatgaactaa
      961 tttcatggca attttctata atctaataat tattaaattt taacaaacct ttattagcat
     1021 tccataatta tttaaaattc tttcaaatac cctatagttt ataggtgaaa gtatctttaa
     1081 tattatatca aacttattat ctttaaatag taaatttgtt aaatctgaaa tacaccatac
     1141 aattttattt tcctcttttg tagctatatc aattctgatt aacttacatc tataataaat
     1201 atctttatct tcatatagtc tattttaaaa attactctta aaaaatcaaa tggcaaataa
     1261 attaattatc cttttaatag aatgtaatca tattatttac tatattccat tatgtactct
     1321 ttaaatcctg aatcttcatt tacttgttct tttactatat taaaaccttt tttattataa
     1381 aacacaactg cttttttatt atctttatat actgctaagc ttagactttt atatttttta
     1441 gttgcataat ctaaaagttt acctccgata cctagatttt ggtatttggg ctttataaat
     1501 aaagctccta taaagctttt atctattatg cttataaatc cttttatttc atctccatca
     1561 tcatatacaa atgtatctga tataggtata tattcgtttt taacagtatt atagttattt
     1621 tgccagtatt ctttacttat aaagtcatgt gctttgattg tacttttttc ccatatttcc
     1681 atgattttat ttatgtcttt attgtttaat ttctttatca taaattttat ttcacctcat
     1741 ttttgtattg attttataat gtttataggt aagtgtattt tatttagata tgatttagtt
     1801 aatataaggt ggatgtttta gtatatgcta ttgttgattc tacaatttta tatactgctt
     1861 tattaatata aaataagttt ctaaatactc gttgatattg ctaatattct acttatctac
     1921 ccttagttga tataaaacct gacactgtgc cttgtgtgag tcttttcttt atcctattta
     1981 cataccactt agttaatata aaacacttcg aaattgcagt tttcagcatg ttattgcgca
     2041 atttacatac cacttagtta atataaaaca aattcgaggt cttgattttg aagtttttgg
     2101 ttatgcattt acataccact tagttaatat aaaacacaaa caagagtagt tgcaatagca
     2161 ctaaaaattt catttacata ccacttagtt aatataaaac tttgcattat attagttaac
     2221 ttttcccata ttttcccatt tacataccac ttagttaata taaaacatat aaatgctaga
     2281 gaaatggtag tcgtaaatga caaatttaca taccacttag ttaatataaa actgaactca
     2341 atattttcca cgttcatgaa aagttcttat ttacatacca cttagttaat ataaaattga
     2401 ttttgctggt aacgttatag atggatttag agaatttaca taccacttag ttaatataaa
     2461 accatttgta aatctttatg attatgtaat atattcataa tttacatacc acttagttaa
     2521 tataaaacgt gtagcaaaaa gtaaactggg ttgtaattat gtgtaattta cataccactt
     2581 agttaatata aaacaacttc tcacattcca gataattgtt attttactcg atttacatac
     2641 cacttagtta atataaaact tttatagctt gccacttaaa agaagaacct tgtcttattt
     2701 acataccact tagttaatat aaaacgaaaa ggatttaaag caggaagtac tagctataca
     2761 acatttacat accacttagt taatataaaa ctgaacttag aaatagaaga aaaagaaaat
     2821 ttatataatt tacataccac ttagttaata taaaacacaa taaatcttgt ctcgtactca
     2881 ttacaatctt ttatttacat accacttagt taatataaaa caagatagat acaaaatgat
     2941 gcaggttaca ggtatgcatt tacataccac ttagttaata taaaactttt ttggtatagt
     3001 agatagaatg ataaaaatac actatttaca taccacttag ttaatataaa acgtcctcta
     3061 aagtttgaaa taagtcaaca aaatatttat ttacatacca cttagttaat ataaaacatg
     3121 ttagaagatg caattgcgga agtggcttgg tcttatttac ataccactta gttaatataa
     3181 aactaaataa aaatgaagta gggggtattt tatggaaaga tttacatacc acttagttaa
     3241 tataaaacct ttttgttgtt ggttttgttg ttggtttttc ttgttattta cataccactt
     3301 agttaatata aaactgttgc atggttaggg gctggagggg ttccctaggc aatttacata
     3361 ccacttagtt aatataaaac ccacgcattt tagttcaaat tcgtagcttt tggcttattt
     3421 acataccact tagttaatat aaaaccagca acatgaattt tcctagcata atcttttaaa
     3481 agttcattta cataccactt agttaatata aaacgtttgt gtgtcctcac tgttccagca
     3541 ttagggggct atttacatac cacttagtta atataaaact tagtgcatct gtaacactca
     3601 tgtccccaaa agccccattt acataccact tagttaatat aaaactataa atgctttgat
     3661 gctatttttg acaagttttc caatttacat accacttagt taatataaaa ccccaaaata
     3721 ggtatatttg aatacattga aaacacttca atcaagcaat tataattcaa taaaaacatc
     3781 aaaatttgca gtgagcgagc agtagtgcaa ttgataacag ttatcataca ccttcaatcc
     3841 cttatattcc aactgttaac acctatttta gcataaatat cgctcactgc aaacctatat
     3901 aaacatttca tcagaatact tatcaatccc ataacatttc tttttaattg aattaggatt
     3961 ttttatctca taaacataga ttgaatcctc gctattatca ataatatttt ctatttcaga
     4021 aatacattta tgtaactttc cttcagtaat attgccttca aaaacagaat tttgtgtcca
     4081 agtcaaatat tttctaagta tccttctaat tctatttaac gaccttgctt caacaatatc
     4141 ataagtaaca ataacaaaca tacttaccac cacaccttta aaactttgta cctttcatca
     4201 tttataaaat gtttaattaa tttataacac tctaacctta taaacattct atatgaaact
     4261 tttctattta gatttctatg ttttatagtt gtctgcatct tattttcaaa ttcttgcaaa
     4321 aatttcttct ttcctttttc acttaaataa caaattttgt cttgatattc aaaatcattc
     4381 ttattaattc ttttattatt aatcaggcta aaaattattg tgtcaactat aattggctta
     4441 aaaatttctg caatatctaa acttaaagaa aatctctttg ttgatggttc atgtaaaaag
     4501 cttattgtag ggtcaagttg tgtcttatat atctcactta aaacattcgt atacattatg
     4561 ctatttccaa aagacataag agcatttact gggtctttgg gaggtctttt ttctctttta
     4621 taaaattcaa aatcatcttt taatatttga ttaaatgatt tatagtatgt ttttcttatt
     4681 ctgccttctt tacccataag ttctgatatt tctttgacat cattcaaatc atttagttca
     4741 tcctttatag tatcaattaa actttcatca actttgtaat atctcatatt tcttaaaata
     4801 tggtatgatg cactttccac aaaggatttt gctaagtata atctttcatt tttatctaaa
     4861 tagcaggctg attgacatac taaactgaat cctgatacat ttttcttcct tggataatat
     4921 gtacctgaat aatatccata gtaattgtag aaatgaagca tcaccccata tttacttatg
     4981 taatttataa gttttgtgtt taaatctact tctccatata tatgaatatc atcaacttgt
     5041 tctactggta atattttttt ctcaccttct aaattgtaat agtaaatcgt attttctttc
     5101 ctttttatat ccccattact tataatataa tagtctttat acattgttac tcctccttca
     5161 caaaacaaaa ttcataataa gcacattttg tacaataatg ttttttctca attttggaag
     5221 gagataactg catatttatt tcatttatat ttataagtgt gttttcaata attctttctt
     5281 tttcttctgt caatatcaat tcttcttgtt tcttttcctt aacataattt atagttcctt
     5341 ttttctctat acccatacta ttctttaaat agtataagta atagtataac tgcatttcat
     5401 ctgcttcttt catctttgaa cttattttta cttcttttac atactcatcc tttattatat
     5461 ctagcttcaa gatatcatca atcaatattt ctttattttt cattctttta tatgaatctt
     5521 catgtactat cttcccagac aatactctac tactattttg ttccatggtt attcctttag
     5581 aaaatagcca tagttttctt ttacatacat aataataatt tatttttaca ccttgaactt
     5641 ttaatacttc taaatctatt ggcacaaaat caccttcttt agtaaatttt agccacattg
     5701 ttaatataaa aatcaaataa attggtctat ttcattactt ataagcacac ctttaccaaa
     5761 tatctgtttt gtatgcttat tttcgtatat atcatattta tataaaagta tttttaaatc
     5821 ttcaagacct ttcacctgta tgtcaattac attttgttta gctttatata ttggtaaatt
     5881 tacagttttt ttaattatct ctcttcttgc tttttttctc ttagacttta ttttatttat
     5941 caattgtcta tcatcattac tatatgcatt tataagctct tgtcccaact cttcataatc
     6001 tttgattaaa gtttcttcaa ttttattata tatatcctct ggaattactg tatatccttc
     6061 aatatttcta agtatatttt gtgcatcttt agaacctaaa ctatatggcg ttatagtatc
     6121 taaaatattt aaagcactgg taaatctctt ttcaaaagct gtaccttcta gactttcttt
     6181 agagtatagt atcttaacca tttctacctt atatttttct ttcatcttca cacattcttt
     6241 tccatttata aaagttttca gtagctcaag tcctttttct acaatatcct tatcataaat
     6301 actacctatt cctgtagctt tttctgtata tataaatata tttgggctat tttcttcata
     6361 ctcacgactt ctataacatc taccaaatcg ttgaaataga ctgtcaagtg ttgaattttc
     6421 tgtatgaagc tcatcaaaat caatatcaag agatgcctct actaattgtg ttgtaatcca
     6481 aattccattt ctatcactat tggcaaattc ttttatatat ttttctaatt ttgctctatc
     6541 ttcttgtata tacatagaat gtaaaagatt taagttgaca tctattcctt ttggtttaac
     6601 tatttcttct attaactcat atttttctac agcactttta actgtattta ctattacaag
     6661 aactttttta ttcattccac tttgtattat tttacctaag ttttcatcta ttgaattttc
     6721 tacaatagaa acacaatgtc ttattttttc tgtattacat gtcaattcag ctaagttact
     6781 attcattaca cctctttttt ttaattcatc tatatatatg gttggcatag tagctgtcat
     6841 tatcataaat ctgccaccta tcttatgtat catttctata ccttttacca atacagctgc
     6901 tatttctggt gaatatgctt gtatctcatc tattactact tttgaatatg ctaatgttga
     6961 gtacactttt tcataccctc tatacaaaaa aggaaattta aatatttggt ctattgtaga
     7021 aaatgtcagt ttgcaagata ataactttgc taaatctaca atctcacttg aattttcttg
     7081 attactttct tctagataat ctattgctgt tgaatgtaac aaacctaaga atgtatcatt
     7141 tgattctcca actccaacta tattttttgc tctatcaaat aatgcattta tacttactct
     7201 taatggaagt gtaaaaaatg ccttgtcttt atctatccaa attaaggcag tttctgtttt
     7261 tcccattcct gtagatgcaa tcagtattat attcttattt ctattagatt tagcaaatga
     7321 ttgagcctct ctcaaactac caaactcttt cattaaataa ttttctgtct gttctcctat
     7381 atttataaca ttattgcatt ccacaacttc atgagcagaa gcactatggt ctagtctatg
     7441 tagtat
//
LOCUS       DAELLV010000181         7426 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.181, whole genome shotgun sequence.
ACCESSION   DAELLV010000181 DAELLV010000000
VERSION     DAELLV010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7426
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.181"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            35..373
                     /locus_tag="KPD58_002869"
     CDS             35..373
                     /locus_tag="KPD58_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HBG0266603.1"
                     /translation="MNLLVIGGHERMEKDYMVMAKKKGYKTKVYTTMSSKLNNSIGRP
                     DAVVILTSTVSHKMSRTVESQAKKQDILIVRHKNSSKVAFNECLDMVDECLGNCSKCK
                     LKDKQKQKNI"
     gene            complement(500..1789)
                     /locus_tag="KPD58_002870"
     CDS             complement(500..1789)
                     /locus_tag="KPD58_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBG0266604.1"
                     /translation="MKRNLSLLLICLLIFTSFLGSSNISFADNEPAIVAKHAVLMDYE
                     SGKILYNKDGNSKLYPASTTKVWTACLVLKEVKDLNQVIEIKDLPQIDGSSMYLKNGE
                     SFTVKQLLDALLVHSANDAAFVLARYVGGGNVQKFIDLMNSEAKKIGATNTHFNNPHG
                     LPDPNHYTTAHDMALIAREAMNNDTFRQIVKTKSLKFEATKAYPYERYFVNTNKFLTS
                     HDKITYKGQPINIKYDIVDGIKTGYTDAAGKCLLSSAVKDGRRVIVAVFNSTNADLYL
                     DSRILIDYGFDNFKCATIVDKEKYTDTKKVLFTKQHELIYEPKNSYRIFLEKDESQGN
                     YDTKTELNKIDLPIKKGAKVGTLNVYNNGKLENSIDLIAKNNLDSSLPFLTENNVLMT
                     FVKIIAGILVLLVLFIIASNIKKKKRKKARSKKNMKK"
     gene            1869..2573
                     /locus_tag="KPD58_002871"
     CDS             1869..2573
                     /locus_tag="KPD58_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBG0266605.1"
                     /translation="MKKKVKFISFFVLFILIISSIIVYKSLIFKDDIYVVSKKNLPNE
                     KTHVVENEIKGEDNNHSKVTKDIDKNFQNNKITIYISGAVNRPGIVTIESDKRLYDAV
                     ELLGGTTKEADLNGVNLSVRLEDEQHYIIPKIGEATSVTSNDDSNKPNQKNESRGEPK
                     NKSNKNISNESKVNINVATIEELDSLPGVGEATANKILQHREENGQFSSIEEIKNVNG
                     IGDKKYENIKDLICVD"
     gene            2665..3711
                     /gene="selD"
                     /locus_tag="KPD58_002872"
     CDS             2665..3711
                     /gene="selD"
                     /locus_tag="KPD58_002872"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2713..2715,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HBG0266606.1"
                     /translation="MKKEQKKLTEMTTAGGUAAKIGPEVLASVLSQLPKNDNIENLLV
                     GLDTADDAAVYKLNDDMALIQTLDFFTPMVDDPYVFGQIAASNSLSDVYAMGGKPLVA
                     MNIVCFPSCHDMDVLAEILKGGFDKVKESGALLVGGHTVDDKEPKYGLSVSGIVSPNK
                     VLSNATAKPGDKLIITKPIGVGVLNTAMKEGMVEQHIADKVIEIMIHLNKYAAMSFEK
                     FDVNSVTDITGFGLLGHTLEMAKASEVSIEIESKHVPIIEGAIEMAQMGIIPAGMYKN
                     MHYVSKDVEVVGNIEVAVQDILYDPQTSGGLLISVKEELAEELVKDMKLNGAIEAKII
                     GSVVPKGEKYITVL"
     gene            3729..5141
                     /locus_tag="KPD58_002873"
     CDS             3729..5141
                     /locus_tag="KPD58_002873"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HBG0266607.1"
                     /translation="MIVLSKRELFAMLPSVDEVLSDNRIVEIINEYPRSLVLESVREV
                     IDLKRQFILRLKEDASNSVTIEFGEIIESAIERVKLNYSLSLKKVINATGTVIHTNLG
                     RSLLSEDIKDELWCAASRYSNLEYDLDNGERGSRYSHLTSTIKRLTGAEDVLVVNNNA
                     AAVLLVLSTMAKGGEAIVSRGELVEVGGSFRIPSIMALSGAELVEVGSTNKTHLKDYK
                     EAITEDTNVLMKVHTSNYRIMGFTESVSIEELVNLGKKYKLPVIEDLGSGVFIDLSKY
                     GLSYEPTVLDSIRQGADVVTFSGDKMLGGPQAGIIVGKKEYIEKMKKNQLTRALRVDK
                     LTICALEATLRMYLDETKAIENIPTLKMLTYKIEELEVKANKLFEKITALNLNANINI
                     EDGFSQVGGGSMPLETISTKVISITPEHMNVSSLEKKLRLSEAHIIARVYDNKYVLDV
                     RTIFDDEFDVIVEELRKAFN"
     gene            5255..7138
                     /gene="selB"
                     /locus_tag="KPD58_002874"
     CDS             5255..7138
                     /gene="selB"
                     /locus_tag="KPD58_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HBG0266608.1"
                     /translation="MKNVVIGTAGHIDHGKTTLIKALTGRETDTLDEEKKRGISINLG
                     FTYFDLPNNRRAGIVDVPGHEKFIKNMLAGASGIDIVLLVVAADEGVMPQTVEHLDIL
                     SFLNIKNGIVVLTKSDTVDDEFRELVKEDICEKIKGTFLEDAEIIEVDSISKRGIDTL
                     IEKIDKMSDDIEDKNENSPARLNIDRVFSIKGFGTVITGTLIEGKISIEDDLVIYPQV
                     LKTKIRSIQVHGENKDTAYAGQRTAINISNVKVDDIKRGDVLAAPNSLEESMMLDVKL
                     SLVNHLDKGLKHWDRLRLYHGTREILCRAVPLDKELIESGESGYVQLRLEESIVSKKG
                     DTFVVRRYSPMETIGGGVIIDPSPKKHKKFDEKVIEALKIKEKGELKDIIEEYLKRNL
                     KNYPNIKEIMSYSGAHEEDVKRALETLISEDKVFIIGNMYMHINQYNKLKENTIKLLS
                     EYHKKYRLRKGILKEEVRSKIESNFKTREMDILLEKLSTENAIKIENNIVSLLDFEVI
                     LNDKQKEIAKKIEKRLKSCGVSSILTIDEVSEGNHNYAEVLESMIGNKVEKLDDLYIM
                     DKDIYENAKNILINYIKENKEITLGEYRDLIDSSRKNCMIILENFDRNKITKRVENKR
                     ILF"
     gene            7243..>7426
                     /locus_tag="KPD58_002875"
     CDS             7243..>7426
                     /locus_tag="KPD58_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266609.1"
                     /translation="MKNIIPDVKPDIKEREDMSLLFIIMGSVLLLFIIKDIEKYETLL
                     HAIYTIFLVATGVIIFN"
BASE COUNT         2988 a          812 c         1317 g         2309 t
ORIGIN      
        1 gaaaatcaat atcaaataaa tgggaggaaa tctaatgaac ttattagtga ttggtggtca
       61 tgaaagaatg gaaaaagatt atatggttat ggccaagaaa aaaggttata agacaaaggt
      121 atatactacc atgtcatcta aattaaataa ttctatagga agaccagatg cagttgtcat
      181 acttacatca actgtttcac ataagatgtc tagaacggtt gagtctcaag ctaaaaaaca
      241 agatatatta atcgtaaggc ataaaaatag tagtaaggta gcatttaatg aatgtctaga
      301 tatggttgat gaatgtctgg gaaattgttc aaaatgtaag cttaaagaca aacaaaaaca
      361 aaaaaatata taggtataaa aaatacataa acataaaatt aaaagtactg agataatagt
      421 tataattatt atctcagtac ttttagattt ataaggataa gtatagtata aattttttat
      481 tataatttcc aactacttgt tactttttca tatttttttt acttctagct ttttttcttt
      541 ttttcttctt tatatttgaa gctattataa ataaaactaa aagcactaga atacctgcaa
      601 taatcttaac aaaagtcatt aaaacattat tttcagtcaa aaatggaaga ctgctatcaa
      661 gattattttt tgcaattaag tctatactgt tttctagttt accattatta tatacattta
      721 aagtaccaac cttagcccct ttttttatag gcaaatcaat tttatttaac tctgttttgg
      781 tatcataatt gccttgagat tcatcttttt ctaagaatat cctataacta ttttttggtt
      841 cataaatcaa ttcatgttgt tttgtaaaca atactttttt agtatctgtg tatttttctt
      901 tatcaacaat agtagcacac ttaaagttat caaatccata atcaatcaga atacgtgagt
      961 ctaaatataa atcagcatta gttgaattaa atacagcaac gataactctt ctgccatctt
     1021 ttacagcact cgataaaaga catttaccag cagcatcagt atatccagtt tttataccat
     1081 caactatatc atattttata tttataggtt gacctttgta tgtaatctta tcatgtgatg
     1141 tcaaaaactt attagtgttt acaaagtatc tctcataagg atatgctttt gtagcttcaa
     1201 atttaagtga cttagttttt acaatttgtc taaaagtatc attattcata gcttctctag
     1261 ctatgagagc catatcatga gctgttgtgt agtgatttgg gtctggtaat ccatgaggat
     1321 tattaaaatg agtattagta gctccaattt tttttgcctc agagttcata aggtcaataa
     1381 atttttgtac attgccacca ccaacatatc tagctaatac aaaagcagca tcattagcag
     1441 aatgaaccaa aagagcatct agtaactgct ttacagtaaa agattcccca ttttttagat
     1501 acatactaga accatctatt tgaggtaaat ctttaatttc tattacttga tttaaatctt
     1561 taacctcctt tagaactaaa caagctgtcc atactttagt tgttgaagct ggataaagct
     1621 tagaatttcc atccttatta tatagtattt tgccagactc atagtccatt aaaactgcat
     1681 gtttagcaac tatagctggt tcattatctg caaaagagat attagagctt cctaaaaatg
     1741 aagtaaatat caataaacag attaataaaa gtgataaatt tcttttcatt gtgagcctcc
     1801 aaattttaaa ttcatctttt agtatagcat atatttgtca ataattaaaa gaaaggagtg
     1861 attaatatat gaaaaaaaag gtaaaattta tttcgttttt tgttttattt attttaataa
     1921 tatcatcaat aatcgtttat aaatcgctta ttttcaaaga tgatatttat gttgtaagta
     1981 aaaaaaattt acctaatgaa aaaacacatg ttgtagagaa tgaaattaaa ggtgaagata
     2041 ataatcacag taaagtaact aaagacatag ataaaaattt tcaaaataat aaaataacta
     2101 tatacataag tggagcagta aatagacctg gaattgtaac tatagagtca gacaagagac
     2161 tttatgatgc tgtagaatta ctaggaggaa cgacaaaaga agcagattta aatggagtca
     2221 atctatcagt aagattagaa gatgaacaac attacataat acctaaaata ggtgaagcaa
     2281 catctgttac aagtaatgat gattccaata aaccaaatca gaagaatgag tcaagaggtg
     2341 agcctaaaaa taaatccaat aaaaatatat ctaacgaatc aaaagtaaat ataaatgtag
     2401 cgactataga agagttggat tctctacctg gggttggaga agctacagcc aataaaattt
     2461 tacaacacag agaagaaaat ggacagttta gttcaattga ggaaataaaa aatgtaaatg
     2521 gtattggaga taaaaagtat gaaaatataa aagatttaat ttgtgtagac taaaatataa
     2581 tacaaaactt acatatagat atgttattat aaatatgtat gtatatgtgt gtacatgtgg
     2641 aaatttagta aaggaggcac ttttatgaaa aaagaacaaa aaaaacttac agagatgaca
     2701 acagcaggtg gttgagccgc taaaataggg ccagaggttc tggcatcagt tttatctcaa
     2761 ttgcctaaaa atgataatat agaaaattta ttagtaggac tagatacagc agatgatgct
     2821 gcagtttata aattaaatga tgatatggca cttatccaaa ctttagactt ctttacacca
     2881 atggtagatg acccatacgt atttggtcaa attgctgcat caaattcact atcagatgta
     2941 tatgctatgg gaggcaaacc tttggttgct atgaatattg tttgttttcc atcatgccat
     3001 gatatggatg tacttgcaga aatattaaaa ggtgggtttg ataaagtgaa agaaagtgga
     3061 gcacttttgg ttggagggca tactgtagat gacaaagagc ctaaatatgg tctttcagtt
     3121 tctgggattg taagtccaaa taaagtttta tcaaatgcta ctgcaaaacc tggtgataag
     3181 ttaattataa caaaaccaat tggagtgggt gtattaaata cagccatgaa agaaggtatg
     3241 gtagaacaac atatagcaga taaagtaata gaaataatga tacatttaaa taaatatgca
     3301 gcaatgagtt ttgagaaatt tgatgtaaat tcggttacag atataactgg ttttggtcta
     3361 cttggtcata ctttagaaat ggccaaagct tctgaagtaa gtattgaaat tgaatcaaaa
     3421 catgtaccaa ttattgaagg tgcaatagaa atggcacaaa tgggaattat acctgctggt
     3481 atgtataaaa atatgcatta tgtatctaaa gatgtagaag ttgtaggaaa tatagaagtt
     3541 gcagttcaag atattttata cgacccacaa acatcaggag ggttgttaat ttcagtaaaa
     3601 gaagaacttg cagaggagct tgtaaaagat atgaaattaa atggagcaat agaagctaaa
     3661 attataggtt ctgtagttcc aaaaggggaa aaatatatta cagttttata aaatattata
     3721 gagaaagagt gattgttttg agcaaaagag aattgtttgc aatgttgcct tcagttgatg
     3781 aagtattaag tgataataga attgtagaaa taataaatga atatcctaga agtttagttt
     3841 tagaatctgt gagagaagta atagatttaa aaagacaatt tatattaaga ttaaaagaag
     3901 atgcatcaaa ctctgtaaca atagagtttg gtgaaataat agaatctgca atagagagag
     3961 ttaagttaaa ttactcatta tctttaaaaa aagttataaa tgcaactggt acagttattc
     4021 atacaaattt aggaaggtct ttacttagtg aagatataaa agatgaatta tggtgtgctg
     4081 cttcaaggta ttcaaactta gaatacgatt tggataatgg agaaagaggt tcaagatact
     4141 ctcatttaac tagtacaata aaaagactta ctggagcaga agatgtacta gttgtaaata
     4201 ataatgcagc ggcagtactt cttgtactta gcacaatggc taaaggagga gaagcaattg
     4261 tatctcgtgg agaattagtt gaagtaggtg gttcatttag gattccaagt ataatggctc
     4321 ttagtggtgc agaattagtt gaagttggtt caactaataa aactcatttg aaagattaca
     4381 aggaggccat tacagaagat acaaatgtat tgatgaaggt tcatacaagt aattatagaa
     4441 taatgggatt cacagagagt gtttctatag aagaattggt taatttaggt aaaaaatata
     4501 aactacctgt aatagaagac ttaggaagtg gagtttttat agatttatca aaatatggtt
     4561 tatcttatga accaactgta ttggattcta ttcgacaagg tgcagatgta gtaactttta
     4621 gtggagataa gatgcttgga ggtccacaag caggtattat agttgggaaa aaagagtata
     4681 tagaaaaaat gaagaaaaat caacttacta gagcacttag agttgataag cttacaatat
     4741 gtgctcttga agcaacattg agaatgtatt tagatgagac aaaagctata gaaaatatac
     4801 ctactttaaa aatgttgaca tataaaattg aagaattaga agtaaaagca aataaactat
     4861 ttgaaaaaat aactgcttta aacttaaatg caaacataaa tatagaagat ggattttcac
     4921 aggtgggagg agggtctatg cctcttgaaa ctataagtac aaaggtaata tcaataactc
     4981 ctgaacatat gaatgtttca tctttagaaa aaaaactaag acttagtgaa gcacatataa
     5041 tagccagagt gtatgataat aaatatgtac tagatgtaag aactatattt gatgatgaat
     5101 ttgatgtaat agttgaggaa ttgagaaaag catttaatta aataaatttg agttaataaa
     5161 tattaaattt agaagtataa gtatgaagtt ttgattaaaa aatagctaca cacacttgtg
     5221 actttagtca tagcaggttt agagggggca aaaaatgaaa aatgtagtaa ttggaacagc
     5281 aggtcatatt gaccacggaa aaactactct tataaaagca ttgactggaa gagagactga
     5341 tacgttagat gaagaaaaaa agagaggtat ttcaataaac ttaggattta cttattttga
     5401 tttaccaaac aatagaagag ctggaattgt tgatgtccca ggacacgaaa aatttataaa
     5461 aaacatgtta gctggagcat caggaattga tatagtttta cttgttgtag cagcagatga
     5521 aggggttatg ccacagacag ttgagcattt agatattcta agctttttaa atatcaaaaa
     5581 tggaatagtg gttctaacta agtctgatac tgtagatgat gaatttagag agctagtaaa
     5641 agaagatatt tgtgaaaaaa taaaaggaac ttttcttgaa gatgctgaaa taattgaagt
     5701 tgattctata tctaaaagag gtatagacac tcttattgaa aaaatagata aaatgagtga
     5761 tgatatagaa gataaaaatg aaaactctcc agcaagactt aatatagaca gagtattttc
     5821 aataaaaggg tttggtactg taataacagg cactttaata gaaggaaaga taagtataga
     5881 agatgatttg gttatatatc cacaggtatt aaaaacaaaa ataagaagta tacaagttca
     5941 tggagaaaat aaagatacag cttatgcagg tcaaagaaca gcaatcaata tatcaaatgt
     6001 aaaagtggat gatataaaaa gaggagatgt tttagctgca ccaaactcat tggaggaatc
     6061 aatgatgtta gatgtaaaat tatcattggt aaatcattta gataaaggat taaagcactg
     6121 ggatagatta agattgtatc atggaactag agagatactt tgtagagcag ttccactaga
     6181 taaagaatta attgaaagtg gagaaagtgg ctatgttcag ttgagacttg aagaaagcat
     6241 agtatctaag aaaggtgata cgtttgtagt gagacgttat tctccaatgg aaactatagg
     6301 tggaggtgta ataattgacc catctcctaa aaaacataag aaatttgatg aaaaagttat
     6361 agaagcatta aaaataaaag aaaaaggcga actaaaagat ataatagaag agtacttaaa
     6421 aaggaattta aaaaattatc caaatataaa agaaataatg agctatagtg gtgctcatga
     6481 agaagatgta aaaagagcgc ttgaaacact tatatcagaa gataaagtat ttattatagg
     6541 aaatatgtat atgcatataa atcaatacaa taaattaaaa gaaaatacaa taaagctttt
     6601 aagtgagtac cataagaagt ataggcttag aaaagggata ctaaaagaag aagtaaggtc
     6661 taagatagag agtaatttta agactaggga gatggatatt ttattagaaa aattgtctac
     6721 agaaaatgct ataaaaatag aaaataatat agtatctcta ttagattttg aggttatttt
     6781 aaatgataag caaaaagaaa tagcaaaaaa aatagaaaaa agattaaaat catgtggagt
     6841 ttcttctata ctgacaatag atgaagtttc tgaaggaaat cataactatg ctgaagtatt
     6901 ggagtcaatg ataggtaata aagtagagaa attagatgat ttgtatataa tggacaaaga
     6961 tatttatgaa aatgcaaaga acattttgat aaattatata aaagaaaata aagaaataac
     7021 attaggagaa tacagagatt tgattgattc tagcagaaag aattgcatga ttatattgga
     7081 aaacttcgat agaaacaaaa taactaaaag agtagaaaac aaaagaattt tgttttagta
     7141 gaaaatgtct gtttttgctt taaggtataa agaaggaatt ataatgtcga gtatcgtata
     7201 attaattagt ataaaatatt aattaagagg aagtgtaaag tattgaagaa tataatacct
     7261 gatgtaaaac cagatataaa agaaagagaa gacatgagcc ttttatttat aataatgggc
     7321 tcagtacttt tattatttat tataaaagat atagagaaat atgaaacttt attacatgcc
     7381 atatatacga tatttttagt agctacagga gtaattatat ttaata
//
LOCUS       DAELLV010000182         7345 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.182, whole genome shotgun sequence.
ACCESSION   DAELLV010000182 DAELLV010000000
VERSION     DAELLV010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7345
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.182"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            138..767
                     /locus_tag="KPD58_002876"
     CDS             138..767
                     /locus_tag="KPD58_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266610.1"
                     /translation="MQDKILRDKKANDPWARMTTRNGFSADEIISSFQKAIRRNMVEE
                     ACEFAYELYISSPELEDKLWRRILTISVEDIGMGDPSAVIIINNLNQVRKEYSYADGD
                     RPLFFIHAIRYLCACEKDRSSDLLKNIIIKGFAMGKVPEVMDVALDKHTYRGLEMGRD
                     SFHFLNEASIVIPEKEVDNDYKERYLKILEKYKQEEVIDTAFKFNHWQY"
     gene            902..1228
                     /locus_tag="KPD58_002877"
     CDS             902..1228
                     /locus_tag="KPD58_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0266611.1"
                     /translation="MNILLVCANGASTGVLVEKMKSFCSEHEKLKTKTINIEATSFEN
                     LKSYIEANDTDVVLVAPQIRFKEDDVVEACKNYKIAVGLIDTKHYGRMDAPSVMKSAI
                     ELYKNR"
     gene            1244..2533
                     /locus_tag="KPD58_002878"
     CDS             1244..2533
                     /locus_tag="KPD58_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0266612.1"
                     /translation="MEALQNNLRRFLLPIAQKIEKQRHLQAIKEGMISITPIIIVGSL
                     SMLFMALNNMLPEGSAKTLLSENMDTLLIPNKFTMSLLSIYSAFFIAQALAKKYNLNH
                     VEIGMTAVVAQLVVCGQVVDGVLDTSYLDAQGLFVSILVALLVVDITKFMNDKNLVIR
                     FPKEVPSVVNKSFRNLTPMIVCIMLFTAIAAITKNVSGQPLPAIIMNFLAPAISSVDN
                     VFAVTIILFITQLLWFFGLHGAAITSSIWMPIAATYMAENATLIAAGGDPKYVFTIGF
                     YYGFLQVTGSGITLGLVYLMSRSKCKSFNSMGKVVILPSLFGINEPVIFGTPIVMNPY
                     MFVPFVFGPVLVGALNFMALKVGLVGLPIAEPPGFLPPGVAAFLMTLDWKAIVLVFAS
                     IILMTLIYYPFFKIMEKEELNKNAELATTLDDDSFDF"
     gene            2557..2886
                     /locus_tag="KPD58_002879"
     CDS             2557..2886
                     /locus_tag="KPD58_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG0266613.1"
                     /translation="MVPEMEEKVFSIISEAGDAKSDVMMSLKEIKKGDYNKAKHLLNS
                     ASEKIQTASKHHLELLSNAMNSEDSGTDFLVVHSEDHYSNALFAHSLVSELVDIFEMM
                     DPRINKN"
     gene            3034..4362
                     /locus_tag="KPD58_002880"
     CDS             3034..4362
                     /locus_tag="KPD58_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBG0266614.1"
                     /translation="MEKFSVVIAGGGSTYTPEIILMLLDNLDRLPLRAIKLYDNDEER
                     QNKVAKACEILIKEKDPNIEYLATTCPKEAYTDVDFCLAHIRVGKLEMRELDEKIPLK
                     HGVVGQETCGPGGIAYGMRSIEGVLENIDYMEKYSPNCWMLNYSNPASIIAEAVRRLR
                     PNSRVINICDMPIGMEHNIARIAGLKSRKHMDIRYFGLNHFGWYTSIKDKKGNELLPK
                     LVEHIKEYGFINGEEGMKNDKKDSWFETNLFTKEIVKTDLTTIPSSYLKYYLFPDYVV
                     NHSDVNYTRANEVIDGREKEVFGSCALIEKQGHSKGSGLKIGIHAEFIVDLATALAFN
                     TQERMLLIVQNNGAIENIQDDAMVEIPCIVGKDGYEPLSIGKIPTFQKGLIEQQLAVE
                     KLVVDAWIEKSYNKLWQALMLSKTVPSATVAKELLDDLIEANKNYWPKLN"
     gene            4679..5539
                     /locus_tag="KPD58_002881"
     CDS             4679..5539
                     /locus_tag="KPD58_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HBG0266615.1"
                     /translation="MAMKWRDYADDVNLFEDFDKTIKPLTDEQRRKNINTLIAYFSKE
                     VPSKIYNLSNDEIKPRDILRGLLNVYPPKEIAPEILNMLHNLLLIECEERELIDVNDI
                     EEVEEGIAIWRGNITNLRADAIVNAANNKLLGCLQPLHLCVDNEIHSCAGPRLREDCD
                     KIIKKQGHLEYTGDAKITRGYCLPAKFVVHTVGPIVSGGQPSKEQEKQLLHCYKSCLN
                     TIKEIDEIKNIVFCGISTGVFGYPKKEAANLAVSRVRLWLKENPEKNLKVVFNVFTEE
                     EEEKYRRIFK"
     gene            5744..6724
                     /locus_tag="KPD58_002882"
     CDS             5744..6724
                     /locus_tag="KPD58_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0266616.1"
                     /translation="MKNSFKNCISLRNKFIIISIVFLLILLIVSYIFGKLVYNSSVGL
                     EQLIKNEDIVKIFSERKDKPLEKLKNYKTSELMIQSSNNYKLESLFITSNIKTRDTMI
                     LVHGIGSSYYEMLKVAYRYLDKGYNVLVYNQRNTGNSGGDNYTFGLYERYDLDSLVKF
                     VKNKFPEGRLGVHGFSMGAGTAAMHSEINSKDDKVDFYILDSPYSEMKDAIRMGVLEK
                     RIPDILINYVVTCGDLYNKFKSGFWYSDVKPYESVEKSNVPILFIHGTKDTVCNYQNS
                     KKMYDLVKHDKKDLWLIEGIGHVDGFEHDSTVYFNKIFKFIDSNVLSDKS"
     gene            6927..>7345
                     /locus_tag="KPD58_002883"
     CDS             6927..>7345
                     /locus_tag="KPD58_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266617.1"
                     /translation="MKNKNNKDKNMLNIKVLRVLGVLLILFDYTGDTLNFYNFFAKPL
                     VSGNLYKTTGTDCIVFLLFIVECVYLVGRYILKVKINWSEIIENEKGNFINNLLVEKI
                     QEESNIVYNLLNSYVLPNIYKKARGLMFKTYHMLIYI"
BASE COUNT         2942 a          741 c         1325 g         2337 t
ORIGIN      
        1 agtatctaag ttttgatgat aaaaatgatt gaaataacag tgttttaaag tgtctaaaaa
       61 aactggcata atacttgctt atattattaa atgagcaata taaataatta aaatttaaat
      121 aaaaaggagt aaataatatg caagataaaa tattaagaga taaaaaggca aatgacccat
      181 gggcaagaat gactacaaga aacggattta gtgcagacga aataatatcg tcatttcaaa
      241 aagctataag aagaaatatg gtggaagaag cttgtgaatt tgcttatgaa ctatatatat
      301 catcaccaga attagaagat aagctatgga gaaggatact tacaatatca gttgaagata
      361 taggaatggg ggacccatca gctgtaataa tcataaataa tttaaatcaa gtaagaaagg
      421 aatattcata tgcagatgga gacagacctt tatttttcat acatgcaata agatatcttt
      481 gtgcatgtga gaaagataga tcaagtgatt tattaaaaaa tataattata aaaggatttg
      541 caatgggtaa agttccagaa gttatggacg tggcacttga caagcataca tatagaggat
      601 tagaaatggg aagggattca ttccactttt taaatgaagc tagtatagta ataccagaaa
      661 aagaagtaga taatgattat aaagaaagat acttaaaaat attagaaaaa tataagcaag
      721 aagaagttat agatacggct ttcaagttta atcattggca gtactaagaa ttagagtaaa
      781 tattaataag aattaaaata aaataataag aattaaagta aaaactgata ggaattagag
      841 taagaattgg ctgataaaaa attagaaaac aaaagaggtt agatattgta ggagggtaat
      901 tatgaatatt ttattagtat gtgcaaatgg agcttcaaca ggtgtacttg tggaaaagat
      961 gaaaagtttt tgtagtgagc atgaaaagtt aaaaactaaa actataaata tagaggcaac
     1021 atcatttgaa aatttaaaat catacattga agctaatgat acagatgtag tacttgtagc
     1081 accacaaatt aggtttaagg aagatgatgt agttgaagct tgtaagaatt acaaaatagc
     1141 agttggttta atagatacta agcattatgg tcgtatggat gctccttctg ttatgaagtc
     1201 ggcaatcgaa ttatataaaa atagataatt ggggggaata aaaatggaag cacttcaaaa
     1261 taatttaaga agatttcttc ttccaatagc acaaaagatt gaaaaacaaa ggcatcttca
     1321 agctataaaa gaaggtatga taagcattac cccaataata attgttggtt ctttaagtat
     1381 gttatttatg gcattaaaca atatgttacc tgaaggaagt gcaaaaacat tattatctga
     1441 aaatatggat acattactta tccctaataa atttactatg agtttattat ctatatattc
     1501 agcatttttt atagctcagg cactagctaa aaaatataat cttaatcatg tagaaatagg
     1561 tatgacagca gttgttgcac aacttgttgt atgtggacaa gtagtagatg gtgtattaga
     1621 tacaagttat ttagatgcac aaggtttatt tgtaagtata ttagtagcac ttttagtagt
     1681 tgatattact aagtttatga atgataaaaa tctcgtaatt agatttccaa aagaagtacc
     1741 tagtgtagta aataaaagtt ttagaaactt aactccaatg atagtatgta taatgttatt
     1801 tacagcaata gcagcaataa ctaaaaatgt ctcaggtcaa ccactaccag ctattataat
     1861 gaatttctta gcaccagcta taagcagtgt agataatgtt tttgcagtaa ctataatact
     1921 atttataact caattattat ggttctttgg gcttcatgga gcagcaataa cttcaagtat
     1981 atggatgcca atagcagcta catatatggc agaaaatgca acactgatag cagcaggagg
     2041 agacccaaaa tatgtgttta caataggatt ttactatggt ttcttgcaag taactggttc
     2101 agggataact ttgggattag tgtaccttat gtcaagaagt aagtgtaaga gttttaatag
     2161 tatgggaaaa gttgtgattt taccatcact atttggaata aatgagccag taatatttgg
     2221 aacaccaata gttatgaacc catatatgtt tgtaccattt gtatttggac ctgtattggt
     2281 tggagcactt aattttatgg cattaaaagt aggattagta ggattaccta ttgcagaacc
     2341 accaggcttt ttacctccag gggttgcagc atttttgatg acattagatt ggaaggcaat
     2401 cgtattagta tttgcaagta taatacttat gaccttgata tattatccat tctttaagat
     2461 aatggagaag gaagaattaa ataaaaatgc agaattagct acaacattag atgatgatag
     2521 ctttgatttt taacaagtca ttagaaggag aaggatatgg tacctgaaat ggaagaaaaa
     2581 gtatttagta taatatcaga agcaggagat gctaaaagtg atgtaatgat gtctcttaag
     2641 gaaataaaaa aaggtgacta caataaagca aaacaccttt taaattcagc ctcagagaaa
     2701 atacaaacag catcaaagca tcatttagaa ttactatcaa atgctatgaa tagtgaagat
     2761 agtgggactg attttttagt tgtgcactct gaagaccatt attcaaatgc attatttgct
     2821 cattctttag tatctgaact tgtagatata tttgagatga tggacccaag aattaataag
     2881 aattaaattt cttgtaaata gtgcttgaag tttggctata gtttattaaa tcaatagatt
     2941 taataaatga actccctttt actttaaagt actagtattt ataaatttat ttataaatac
     3001 agtaaatttt taaatagaaa aggagatatt attatggaaa aattttcagt agtaatagct
     3061 ggaggaggaa gtacatatac cccagaaatc atattaatgt tgctagataa cttagatagg
     3121 ttaccattaa gagcaatcaa attatatgat aatgatgaag aaagacaaaa taaggtagct
     3181 aaagcttgtg aaatactaat aaaggaaaag gaccctaata tagagtattt agcaacaaca
     3241 tgccctaaag aggcttatac agatgtagat ttttgtttag ctcatataag agttggaaaa
     3301 cttgaaatga gagaactaga tgaaaagata cctctaaaac atggagtagt tggtcaagaa
     3361 acttgtggac caggtggaat agcttatgga atgaggtcta tagaaggtgt tcttgaaaac
     3421 atagattata tggaaaagta ttctccaaat tgttggatgt taaattattc taatccagca
     3481 tcaataatag cggaagctgt tagaagattg agacctaatt ctagggtaat aaatatttgt
     3541 gatatgccaa taggtatgga acataatata gcaaggattg caggactcaa atctcgtaaa
     3601 catatggata taagatactt tggattaaat cactttggtt ggtatacttc aattaaagat
     3661 aaaaaaggaa atgaactttt accaaaatta gtagaacata taaaagaata tggttttata
     3721 aatggtgaag aaggaatgaa aaatgacaag aaagatagtt ggtttgaaac taatttattt
     3781 actaaagaaa tagtaaaaac agatttaact acaataccaa gtagttactt aaagtactat
     3841 ctatttccag attatgttgt aaatcattct gatgtaaatt atactcgtgc caatgaagtt
     3901 attgatggaa gagaaaaaga agtttttggt tcatgtgcat tgatagaaaa gcaaggacat
     3961 tctaagggca gtgggcttaa gataggaata catgcagagt ttatagtgga tttagctaca
     4021 gctctagctt ttaatacaca ggagcgtatg ttattgatag ttcaaaataa tggagctata
     4081 gaaaatatac aagatgatgc tatggttgaa attccatgta tagttggaaa agacgggtat
     4141 gaaccattat caatagggaa aataccaaca ttccaaaaag gtttaataga acaacaatta
     4201 gcagttgaaa aattagtagt tgatgcttgg attgagaagt cttataataa attgtggcaa
     4261 gcacttatgc tatctaagac agttcctagt gcaacagttg caaaagaatt actagatgat
     4321 ttaatagaag caaataaaaa ttattggcca aaacttaatt aagaataata tatttttaaa
     4381 gttcctacag tttagtacat agcttagtta atgtatttgt agggacttta tttatctaca
     4441 aatgagatac aagtggaaaa catagtgtat tttgcagtta ggaaagtagg tttagttact
     4501 tgttagttgg attaaattta gatacaataa tttactatca atcaatagaa ataaccagag
     4561 aagttgcata ataaaaaatt tacactttta acaggattta tgtaggtgtt tatagaaata
     4621 aataaatatg tacataaata cataattatt tttaagtcta aaatacaaga atggagagat
     4681 ggctatgaag tggagagatt atgctgatga tgtaaattta tttgaggatt ttgataaaac
     4741 tatcaagcct ctaactgacg aacaaagaag aaaaaatata aacactttaa ttgcatattt
     4801 ttcaaaagaa gttccttcta aaatatacaa tttatcaaat gatgaaatta agcctagaga
     4861 tatacttaga ggacttttga atgtatatcc accaaaagaa attgctccag agatacttaa
     4921 tatgttacat aatttattgt tgattgaatg tgaagaaaga gagttaatag atgtaaatga
     4981 tatagaagaa gtagaggaag ggattgccat atggagaggt aatataacga atttaagagc
     5041 agatgcaata gtaaatgctg caaataacaa gttattaggg tgtttacaac cattacatct
     5101 atgcgtggat aatgagatac attcatgcgc aggtcctaga cttagagaag attgtgacaa
     5161 aataataaaa aaacaagggc atttagaata tactggagat gctaagataa ctagaggtta
     5221 ttgtttacct gcaaaatttg tagtacatac tgttggacct attgtatctg gtggtcagcc
     5281 aagtaaagag caagaaaagc aactgcttca ttgttataaa tcatgtttaa atacaataaa
     5341 agaaattgat gaaataaaaa atattgtttt ttgtggaatt tctacaggtg tatttggtta
     5401 tccaaaaaaa gaggcagcta atcttgctgt aagtagagtt agactatggc taaaagaaaa
     5461 tcctgaaaaa aatttaaaag ttgtatttaa tgtgtttact gaagaagaag aagagaaata
     5521 tagaagaata tttaaataat attatgtaac agaatcctaa aaatacttat tatataatac
     5581 aaattcattg tataagataa taatttacaa gtagtaaaaa ttaataaaat ataatttgta
     5641 tttttaataa ataagtaggt ataatagaaa ttataagaat agtataatca attatgctat
     5701 tacttagaaa atataatata ggaaaattgt atggtaaata aatatgaaaa atagttttaa
     5761 aaattgtatt agtttaagaa ataaatttat aattatatca attgtgtttc tattaatcct
     5821 attaattgta tcttatatat ttggaaaatt agtgtataat agttcagttg gtttagagca
     5881 gttaataaaa aacgaagata tagtaaaaat attttctgag agaaaagata aaccactgga
     5941 gaaattaaaa aactacaaga catctgaatt aatgatacaa agttctaata attacaagct
     6001 tgaaagtctt tttataactt caaatataaa gacaagagat actatgatac ttgttcatgg
     6061 aattggaagt agttactatg aaatgttaaa ggtagcatat agatatttag ataaaggtta
     6121 taatgtatta gtttataatc aaagaaatac tggtaatagt ggtggagata attacacatt
     6181 tggtctttat gaaaggtatg atttagatag tctagttaaa tttgtaaaaa ataaatttcc
     6241 tgaagggaga ttaggagtgc atggattttc catgggggca gggacagctg ctatgcattc
     6301 agagataaat tcaaaagatg ataaggtcga tttttatata ctggattctc cttattctga
     6361 aatgaaagat gcaattagaa tgggagtact agaaaaacga ataccagata tattaatcaa
     6421 ttatgtggtg acttgtggag atttatataa taagtttaaa tctggatttt ggtacagtga
     6481 tgtaaaacca tatgagtcag ttgagaaatc taatgtaccc atactgttta tacatggaac
     6541 aaaagatact gtttgtaatt atcaaaatag caagaaaatg tatgatttag ttaaacatga
     6601 taaaaaagac ctatggctta tagaaggtat tggtcatgtt gatggatttg aacatgatag
     6661 tacagtatac ttcaataaaa tatttaaatt tatagatagt aatgttttaa gtgataaaag
     6721 ttaattgaat attagtagct tatttatttt tgtatttatt aaaagataga taggctattt
     6781 ttatgtttaa atataagttt tgtttagtaa tgtgaattaa tttctagtaa aaatgaaaat
     6841 aactaccaat taaattcatt tatacctcaa ataaaagagt tagtattaga ttacaatcaa
     6901 atctaaagaa aagagaggta gtatatatga aaaacaaaaa taataaagat aaaaacatgt
     6961 tgaatattaa agtattgaga gttctaggag tgctattaat actttttgat tatacgggtg
     7021 atactttgaa tttttataat ttcttcgcaa agccattagt atcgggtaat ttatataaga
     7081 caacaggtac tgactgcatt gtatttttat tatttattgt agagtgtgta tacctagtgg
     7141 gaagatatat attaaaagtt aagataaatt ggagtgagat tattgaaaat gagaaaggta
     7201 attttataaa taacttgttg gttgaaaaaa tacaagaaga aagcaacatt gtatataatt
     7261 tattaaactc atatgtgttg ccaaatatat ataagaaagc aaggggattg atgtttaaaa
     7321 catatcatat gttaatctac attaa
//
LOCUS       DAELLV010000183         7276 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.183, whole genome shotgun sequence.
ACCESSION   DAELLV010000183 DAELLV010000000
VERSION     DAELLV010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7276
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.183"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            4..858
                     /locus_tag="KPD58_002884"
     CDS             4..858
                     /locus_tag="KPD58_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG0266618.1"
                     /translation="MKKDRITFSEILILIVGCILMAISLNLFFNPHAIAAGGITGLGV
                     VLNSLFGVELWIVNLLLNVPLFIFAYKILSKKDCFKTVLGIIFLTIALKLTANMATLD
                     ITNDMYLAIISGSILMGVGQGLIFRINGSTGGTDLMALLLNKYFPTFSIPVLMGIVDC
                     VVVVLSGIVNRQVEIALYSTVALYILVKVSDLLIEGFNYSKSFTIISDLSKDISKKIM
                     EDLDRGATILKGEGAYTGENKNVLLVVVEKKEVVELKKLVKNVDPNAFIIITDIHEAL
                     GNGFKKIE"
     gene            1314..1880
                     /locus_tag="KPD58_002885"
     CDS             1314..1880
                     /locus_tag="KPD58_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG0266619.1"
                     /translation="MSVSLQKGQRVSLVKDNNPVKNLVVGLGWDMNKLGKKNYDLDAF
                     AIALTNQDKMNVRGDLVYFGNLKHPSKAIIHTGDNLTGKGEGDDEQIIVNLEDIPEYV
                     HKIVFGVSIYKAKKRDQDFGQMNNSFIRLIDSNSKQELFKYNLQEDFSGKVTVLAGEI
                     YRRNEEWKFNALGIGQNEELRELINTYK"
     gene            1920..2501
                     /locus_tag="KPD58_002886"
     CDS             1920..2501
                     /locus_tag="KPD58_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG0266620.1"
                     /translation="MGITLAKGQKVSLTKSNPGLKKVIVGLGWDINKYDGGFDFDLDA
                     SAFLTGTDGKVTNDGDFIFYNNLKHASGAVEYMGDNRTGVGDGDDEQINVDLSKIPQN
                     IAKISFSVTINEAITRRQNFGQVENSYIRIYNEETNEELIKYELGEDFSIETAIVVAE
                     LYRHNGEWKFNALGSGFEDGLAGLCKNFGVNIG"
     gene            complement(2558..3475)
                     /locus_tag="KPD58_002887"
     CDS             complement(2558..3475)
                     /locus_tag="KPD58_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG0266621.1"
                     /translation="MTIRSLEIFVKVAECGKMSEVARNMYITQSSVSQAISEIEKEYG
                     VKLFDRISKKLYLTEAGKKLLGYGRHLLAVNEEMNDCMKHCAKNIRIRVGATVTVGTC
                     VISPIMLELYKVNPLIEPEVFVEDTRLIAKKLLNSELDIAIVEGKIKHPDIVTKSIIN
                     DNLVLICSHKHEFYKRDSIKVSELSNQPLIMRELGSGTRAQLEKQLKELKIPMNIRWS
                     CYNSEAILRAVVDNFGIAVISELLIEDYLKKHLLWACDIEGINLHRTFDIAYHRSKFF
                     TENISAFFDISVEYGKKQARKKSRSVNSL"
     gene            3605..5236
                     /locus_tag="KPD58_002888"
     CDS             3605..5236
                     /locus_tag="KPD58_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG0266622.1"
                     /translation="MKVLIIGGVAAGTKTAAKIKREMGDNCSVTILNKGDDISYAGCG
                     LPYYVGKIIEDKSSLIVNTPESFSKLTGAEVRCGVEVTSIDRNAKQVTLKTLSTGEKE
                     NLSYDKLVIATGADPIKPPIPGIDLPGVFFMRTPNDAIKLRDSIENGIKRAVVIGGGF
                     IGLEIAENLSAMGIRVSVVDMAEHVMPGFDTDFAEYVENHMADKGIMIFTGDQVVGIE
                     GENKVEKLRTKNRAIKTDLVVMSVGIRPNTGFLKDTGLEFAPNNTIIADEYMLTNDKD
                     IYVVGDCAFVKNSLTNKPTWSPMGSSANHEGRICAQNISGKSKTYNGVLGTTVVKLPE
                     LNAGKTGLSKEIAEKEGYNVCSVTIATDDKAHYYPGASNFIIRMVAEKNTRKLLGVQV
                     MGPGAVDKIVDIAATAITLGADLYSLESMDLAYAPPFSTAIHPFVVAVNVLQNKLNGE
                     LDSVLLDEIEDFDSWTKLDVSKAPSIPSLRYIPVGEINGEIEGLAKDEKILLICAKGK
                     NSYMAQNRLRRFGYTNTKVLEGGILFYPNLKVMEE"
     gene            5239..6186
                     /locus_tag="KPD58_002889"
     CDS             5239..6186
                     /locus_tag="KPD58_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HBG0266623.1"
                     /translation="MSNVTAEQKKEVKGRGFLPSRDGEHFAARIITVNGVINASQAKK
                     IAEAAEKFGNGQLAFTTRLTVELPGVKFEDIEALSEFIASENLITGGTGSRVRPVVAC
                     KGTVCVHGLADTQALAAEIHEEFYKNWYDVKLPHKFKIGVGGCPNNCIKPDLNDLGIV
                     GQRVPDYDSELCVGCKKCAVVEVCPVKAAKLTDKGKLEIDSNLCNNCGKCIESCNFDS
                     IEEKESGYKVYIGGKWGKSVRPGTQIDRLFSKEELMTLIEKAILLFREQGNTGERFGI
                     TIDRIGADKFIEMLLSDEVLERKEEILEALLHLTGGAVC"
     gene            complement(6471..6734)
                     /locus_tag="KPD58_002890"
     CDS             complement(6471..6734)
                     /locus_tag="KPD58_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1883 domain-containing protein"
                     /protein_id="HBG0266624.1"
                     /translation="MEYSYSKMNLKKGDIVEVNLEKQANVILLDHINYVKFKNQRNYD
                     YYGGFAKKNPCRMRVPNTGTWYLVVNQDGNSGIVNFSINTIQN"
     gene            complement(6833..7078)
                     /locus_tag="KPD58_002891"
     CDS             complement(6833..7078)
                     /locus_tag="KPD58_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266625.1"
                     /translation="MFIDEELEGYILTCKISEDFKNIPEYSDEEFYVTVYKDESSDSG
                     YYALLENKEERVVWDGEVVANNIFNNLWIVINKVKTG"
BASE COUNT         2628 a          958 c         1146 g         2544 t
ORIGIN      
        1 ttattgaaaa aagatagaat aactttttca gaaattttaa tattaattgt aggctgtata
       61 ttaatggcca tttctcttaa cttatttttt aatccacatg caatcgctgc tggtggtata
      121 acaggacttg gagttgtttt aaattcatta ttcggtgtag aactttggat tgtcaattta
      181 cttttaaatg ttccattatt catatttgct tacaaaatac tttcaaaaaa agattgtttt
      241 aaaacggttt taggaataat ttttcttacc atagcactta agcttactgc aaatatggct
      301 acattagata taacaaatga tatgtatctt gcaattatat ctggttctat tttaatggga
      361 gttggtcaag gacttatttt tagaataaat ggctctacag gtggaactga tttaatggca
      421 ctcctactaa ataaatattt tcctacattt agtatacctg ttttaatggg tattgtagac
      481 tgcgttgtag ttgttttatc aggtatagta aatagacagg ttgaaattgc actttattca
      541 actgtggctt tatacatttt agtaaaagtt agtgacttgc ttattgaagg atttaattat
      601 tccaaatcat tcactattat atctgacctt tcaaaagata taagtaaaaa aataatggaa
      661 gatttagata gaggtgcaac tattctaaaa ggagaaggtg catatactgg agaaaataaa
      721 aatgtacttt tagtagtagt tgaaaagaaa gaagttgtcg aattgaaaaa attagtaaaa
      781 aatgtcgacc ctaatgcttt tattataatt acagatatac atgaagcttt aggtaatgga
      841 tttaaaaaaa tagaataact aacaaataaa tagcaagtat aattaacata tacaaattca
      901 actttttata tgttatatta tacttgctat ttatatatta tacttgctat ttatatatta
      961 taattgttgt ttttaagata aaaattaaat gtatcaatta ttgttttata caagtatttc
     1021 caaatccgtt ctcatttaga tagtatgctt agaaatttta gaattattga gtctaacttt
     1081 attacttact ttactttata acaatcaaat acaaatttaa atattataaa actacaaaat
     1141 aatttaaagc atataatgtc aatatgttta aattataaaa tattcatgta gttatataat
     1201 aaataattta taaatatagc caacttagcc taaatacgta tataatatag ctaatatggt
     1261 tcttatactt gataccatca ataaaactaa aaatataaaa aggagaactt aaaatgagtg
     1321 ttagtttaca aaaaggacaa agagttagct tagtaaaaga taataatcct gtaaaaaatc
     1381 tagtagtagg cttaggatgg gatatgaata aattgggtaa aaaaaattat gacctagatg
     1441 catttgcaat agcattgact aaccaagata aaatgaatgt tagaggtgac ttggtttatt
     1501 ttggaaatct taaacaccct tctaaagcta ttatacacac tggagataac ttaactggaa
     1561 aaggtgaagg tgatgatgaa caaataattg ttaatttaga agacatacct gaatatgtac
     1621 ataaaatagt ttttggagtt tctatataca aagctaaaaa aagagaccag gattttggtc
     1681 aaatgaacaa ctcttttata agattaattg attcaaattc caagcaagaa ttatttaaat
     1741 ataatcttca agaagacttt tctggtaaag taacagtttt ggcaggagaa atttatcgta
     1801 gaaatgaaga atggaaattt aatgctttag gaattggtca aaatgaagag cttcgtgaat
     1861 taattaatac atataagtaa taattacaat aaaaaattta taatgaggag ttggtttaaa
     1921 tgggtataac tttagcaaaa ggacaaaaag taagtttaac aaaaagtaac cctggtctaa
     1981 aaaaagtaat agtaggacta ggatgggata ttaataaata tgatggtggc tttgatttcg
     2041 atttagatgc ctctgcattt ttaactggaa ctgatggaaa agtaacgaat gatggagact
     2101 ttatattcta taataattta aaacacgctt ctggagctgt tgaatatatg ggtgataata
     2161 gaacaggtgt tggtgatggt gatgatgaac aaataaatgt agacttatct aaaatacctc
     2221 aaaatattgc taaaatatca tttagtgtta ctataaacga agcaattaca agaagacaaa
     2281 atttcggaca agttgaaaat tcatatataa gaatatataa tgaagaaact aatgaggaac
     2341 ttattaaata tgaattgggt gaagatttta gcatagaaac agcaatagtt gtggcagaac
     2401 tatatagaca taatggagaa tggaaattta atgctttagg ttctggattt gaggatggac
     2461 ttgcaggatt gtgtaaaaat tttggcgtta atataggcta atatatctaa ataaaaataa
     2521 gagtatattg atatactctt atttttattt agatatatta taaactgttt acacttctac
     2581 tcttctttct cgcttgtttt ttcccatatt ctactgaaat atcaaaaaat gctgaaatat
     2641 tttctgtaaa aaacttgctt ctatgatatg caatatcaaa tgtcctatgt aaattaatac
     2701 cttcaatatc acaagcccac aaaagatgct tttttaaata atcctcaata agtaattcgg
     2761 atataactgc aatgccaaaa ttatctacaa ctgctcttaa tatagcttca gagttataac
     2821 atgaccatct aatattcatt gggattttta attcttttaa ttgcttttct aattgtgctc
     2881 ttgtaccact tcctaattca cgcataatta atggttgatt tgaaagttct gaaactttta
     2941 tagagtctct tttataaaac tcatgcttgt gtgaacatat aagtacaaga ttatcattaa
     3001 taatgctttt tgtaacaata tctgggtgct taatttttcc ttctacaatc gcaatatcta
     3061 attcactatt aagtaatttt tttgcaatca gtcttgtatc ttctacaaat acttcaggtt
     3121 ctataagagg atttaccttg tataattcta acattattgg acttattaca catgtaccaa
     3181 cagttactgt tgccccaact cttatacgga tattttttgc acaatgcttc atacaatcat
     3241 tcatttcttc atttacagct aaaagatgcc tcccatatcc aagaagtttt ttccctgcct
     3301 cagttaaata taattttttt gaaattctat caaatagctt aacaccatac tctttttcta
     3361 tttctgaaat agcttggcta actgatgact gggtaatata catatttctt gctacttcac
     3421 tcattttacc acattcagca accttaacaa agatttcaag actacgaatt gtcatattat
     3481 cacaacctta gcaaacatta gtaaatgctt atgattttat taatatttta gtattttaca
     3541 aatatattgt caagaagtat aattttatta tagagttata aagtagattg gagtgtgata
     3601 tattgtgaaa gtattaatta taggtggagt tgctgctgga acaaaaactg ctgcaaaaat
     3661 aaaacgtgaa atgggtgata attgctctgt tacaatacta aataaaggtg atgatatttc
     3721 ttatgcaggt tgtggtcttc catattacgt tggtaagata attgaagata aatcatcttt
     3781 aatcgtaaat acacctgaaa gtttttctaa actaacagga gcagaagttc gttgtggagt
     3841 tgaagttaca tctattgata gaaatgcaaa acaagtaact ttgaaaacgc tttcaacagg
     3901 agaaaaagaa aatctaagct atgacaaact tgtaattgct acaggggcag acccaataaa
     3961 accaccaata cctggaatag atttacctgg agtattcttt atgcgtactc caaatgatgc
     4021 tataaaacta agagattcta ttgaaaacgg tattaagaga gctgttgtta ttggtggagg
     4081 tttcattggt cttgaaattg ctgaaaatct ttctgcaatg ggtatacgtg taagtgttgt
     4141 tgatatggct gaacatgtta tgcctggatt tgatactgat tttgctgaat acgttgaaaa
     4201 tcatatggct gataaaggca taatgatttt tacaggtgac caggttgtag gtatcgaagg
     4261 tgaaaataaa gttgaaaaac ttcgtactaa aaatagagct attaaaacag atttagtggt
     4321 tatgtcagtt ggaatccgtc ctaacacagg ttttctaaaa gacacaggtt tagaatttgc
     4381 ccctaataat acaattattg cagatgaata tatgcttaca aatgacaaag atatatacgt
     4441 agttggagac tgtgcttttg taaaaaattc tttaacaaat aaacctacat ggtcacctat
     4501 ggggtcatct gcaaatcatg agggtagaat ttgcgcacaa aatatatctg gaaaatctaa
     4561 aacttacaat ggtgttcttg gaacaacagt agtaaaatta cctgagctca atgcaggaaa
     4621 aacaggtcta agtaaagaaa tagctgaaaa agaaggttat aatgtttgct ctgtaaccat
     4681 agctactgat gataaagctc attattatcc aggtgcatct aattttatta taagaatggt
     4741 tgctgaaaaa aatactagaa aattattagg tgttcaagtt atgggacctg gagcggttga
     4801 taaaatagta gatatcgctg ctactgcaat aactttaggc gctgaccttt attcattaga
     4861 aagtatggac cttgcatatg caccaccttt ttcaacagct attcaccctt ttgtagtggc
     4921 agtaaatgtt ttacaaaata aacttaatgg agaacttgat agtgttttac ttgacgaaat
     4981 tgaggatttt gacagttgga ctaaacttga tgtttcaaaa gctccttcta ttcctagttt
     5041 acgttatatt cctgttggtg aaattaatgg agaaattgaa ggccttgcta aggatgaaaa
     5101 aattcttctt atatgtgcta aaggtaaaaa tagctatatg gcgcaaaatc gcttaagacg
     5161 ctttggctat acaaatacga aagttcttga aggtggaata ctattttatc caaatcttaa
     5221 ggtaatggag gagtaaatat gagtaatgtt acagcagaac aaaaaaagga agtaaaaggt
     5281 agaggttttc taccaagcag ggatggagaa cattttgcag cacgtataat aactgtaaat
     5341 ggtgtaataa atgcatcaca agcaaaaaaa attgcagaag ctgctgaaaa attcggaaac
     5401 ggtcagttag catttactac tagactaaca gtagaattac ctggtgtaaa atttgaagat
     5461 attgaagctc taagtgaatt tattgctagc gaaaatctaa taacaggtgg aactggatca
     5521 agggttcgtc ctgttgtggc ttgtaaaggt actgtttgtg ttcatggtct agctgataca
     5581 caggcacttg ctgctgaaat acacgaagaa ttttacaaaa attggtatga tgtaaaacta
     5641 cctcataagt tcaaaattgg tgttggtgga tgccctaata actgtattaa acctgaccta
     5701 aacgatcttg gaattgttgg tcaacgtgta cctgactatg attcagagct ttgtgttggt
     5761 tgtaaaaaat gtgctgttgt agaggtctgt cctgttaaag cagctaaatt aacagataaa
     5821 ggtaaacttg aaatagatag taatctatgt aataactgtg gtaagtgtat agaaagttgt
     5881 aattttgata gtattgaaga aaaagaatct ggttataaag tctatatagg agggaaatgg
     5941 ggaaaatcag ttcgtcctgg aacacagatt gatagattat tttcaaaaga ggaacttatg
     6001 actttaattg aaaaagcaat tcttttattt agagaacagg gtaacacagg tgaacgtttt
     6061 ggaataacta tcgatagaat cggtgctgat aaatttatag aaatgctttt atcagatgaa
     6121 gttttggaaa gaaaagaaga gatactagag gctcttttac accttacagg tggagctgta
     6181 tgctaacaat aaatatttta attgtacaga taacctatgg aaaaaatttt agttaagaat
     6241 aattaaaatt ctatatatga tatatgataa atgaggctat ctcaaaacac agatacgcct
     6301 ctttttcatt gttgaaatga taaagtatat ttgagttcat ataaaaaaga gtgtctcgtg
     6361 aattataaaa ttcattttga ggcacccaca ttattttata ataattgctt attaatttta
     6421 ttttaactat ataaaaagta caaaaagtaa gaatatccaa tattctaaaa ttaattttga
     6481 attgtattga ttgaaaagtt tactattcca ctatttccat cttgatttac tactagatac
     6541 catgttccag tattaggaac tctcattcta catggatttt tctttgcaaa tccaccatag
     6601 taatcataat ttctttgatt tttaaatttc acataattta tatggtctaa caatattaca
     6661 ttggcttgtt tctctaaatt tacctctaca atatcccctt tttttaagtt catcttacta
     6721 taactatatt ccattgaatt actcctccag tatcgaataa tctttgttat ttactcatct
     6781 caaatatgta aaaattcatt taaaatatta caaatactat ataattagac tattatccag
     6841 ttttaacctt atttattaca atccaaaggt tattaaaaat attattggca acaacttctc
     6901 catcccatac aactctttct tctttatttt ctaataaagc atagtaccca gagtcagaac
     6961 tttcatcttt atagactgta acataaaact cttcatcact atattcaggt atatttttaa
     7021 agtcttcaga tattttacat gttaatatat aaccttctaa ttcttcatct ataaacaaag
     7081 caattcctcc aatctctata tttttactaa ttataccata ttatgtcata aactttttat
     7141 tttattacaa atttctattt tttctttact ttataaataa attatataaa ttttcacttt
     7201 ttaactttat attatactaa ttaattcttt tattttccta tatatttctt tataatttat
     7261 aaatttagcc attaat
//
LOCUS       DAELLV010000184         7257 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.184, whole genome shotgun sequence.
ACCESSION   DAELLV010000184 DAELLV010000000
VERSION     DAELLV010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7257
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.184"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(96..701)
                     /locus_tag="KPD58_002892"
     CDS             complement(96..701)
                     /locus_tag="KPD58_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0266626.1"
                     /translation="MKIKENVLMLDSTRGSNCFIVFDKEIILIDTGLPFMGENIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIPFIIGEKDRPGFKK
                     FIGKAISRRLIKDVEPYGENMQIGNIKIIHTPGHTPGHVCMIFEDVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMMKSISELDNLKYEWICMSHGTPCIK"
     gene            880..1518
                     /locus_tag="KPD58_002893"
     CDS             880..1518
                     /locus_tag="KPD58_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0266627.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFDNTTMNELAKEVE
                     YTKRTIYKYFSCKEDLFFAVVLRGYKRLWDNVKIESAKGKTGFEKIKLSYFAFHKFYS
                     VEPSLLCLMGMIGIVNSEKSDTDMLFKEKFFSFNKFMFDEIQGMFEIGKNDRSIRHDI
                     EIPTLMYSSIFTLTGFFNLLSVTGKSYLNNFNIDEEKFIETTLALLIDSIKA"
     gene            1650..2312
                     /locus_tag="KPD58_002894"
     CDS             1650..2312
                     /locus_tag="KPD58_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0266628.1"
                     /translation="MAKILLLEDDDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCENISLNKVEVKAYLDNVEVPLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DN"
     gene            2312..3493
                     /locus_tag="KPD58_002895"
     CDS             2312..3493
                     /locus_tag="KPD58_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0266629.1"
                     /translation="MKTPRNINSTVVFIIFCLIVTFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVVYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTIHAENCHNGGARFIIKFY
                     T"
     gene            complement(3674..6334)
                     /locus_tag="KPD58_002896"
     CDS             complement(3674..6334)
                     /locus_tag="KPD58_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0266630.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIKLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPIAPKSKGESFKKISEATLTIVGKVDVSTSSVIPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYVNSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKNIASSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYDTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKIVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQDASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPE
                     GIKKMLILEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(6331..7014)
                     /locus_tag="KPD58_002897"
     CDS             complement(6331..7014)
                     /locus_tag="KPD58_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0266631.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEIASLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
BASE COUNT         2549 a         1122 c          963 g         2623 t
ORIGIN      
        1 acagctacaa atatacatga tatattaaaa agaagctgta aattattatc atacatacaa
       61 gcaactatgc ctctattaat ccatgttaat ttttattatt taatacaagg agttccatga
      121 gacatacata tccattcata ttttaaatta tccaattcgc ttatagattt catcatcttt
      181 ttataatccc aattccatgg attaggataa ggaactatat ttccattctt atttttgact
      241 aaatcacctg caaataatac atcttcaaaa atcatgcaga catgccctgg agtatgacca
      301 ggagtatgga taatttttat attcccaatc tgcatattct ctccataagg ttcaacatcc
      361 ttaatgagcc ttctggaaat tgcctttcca ataaacttct taaacccagg tctatccttc
      421 tctcctataa taaaaggtat atcctctaca tgagcccata cttttgctcc tgttttttct
      481 ttcaataatt taatattaga tatatggtca acatcatgat gagttaataa aatatgctta
      541 atatctgtaa gtttaatgcc aagtgttttt aattctttaa ttatattttc ccccataaaa
      601 ggaagacctg tgtctataag aataatttct ttatcaaaca caataaaaca atttgagcca
      661 cgagtactat ctaacattaa tacattttct tttatcttca taaataaatt ccccttattt
      721 ggtattttac ctatataatt aattaataaa gttataaata gttataatac ttgatattaa
      781 ttttattttt tattaaagta tactataggt atacaagtat tatatttaca taagtatacc
      841 atcagtatac ttttttatca atagaaagga ataatttata tgtcagaatt atcaaaaaga
      901 gataaagaaa aaatacaaag agaaaatgag attattgata aggcagaaaa attattttgt
      961 cttaatggat ttgataatac aactatgaat gaattagcaa aagaagttga atatactaaa
     1021 cgcaccatat acaagtactt ttcatgtaaa gaagatttat tctttgctgt tgtcttaaga
     1081 ggatataaaa gactttggga taatgttaaa atagaaagtg caaaaggcaa aacagggttt
     1141 gaaaaaataa aactttcata ctttgctttt cataaatttt actctgtaga accttctcta
     1201 ttatgcctta tgggaatgat tggtattgta aactctgaaa aatcggatac tgatatgctg
     1261 tttaaagaaa agtttttttc ttttaataaa tttatgtttg atgaaataca aggaatgttt
     1321 gaaattggaa aaaatgatag aagtattagg catgatattg aaatacctac tcttatgtat
     1381 tcatcaatat ttacacttac aggatttttt aatttactat ctgtaacagg aaaatcatac
     1441 ctgaacaact ttaatataga tgaggaaaaa tttattgaaa caactcttgc tcttttaata
     1501 gattcaatta aagcataaac ctaagtttat aaattaaagt ttatatactt aaatgataat
     1561 tttttctatc tctaatataa aaaatattaa acataaaact aataacttag ttaatggaat
     1621 aactaaatta ctgaaaggat taaaagtaaa tggctaaaat acttctttta gaagatgatg
     1681 atacaattac atttggaata aaaacttccc ttgaaaagaa aaatcacact gtatgttgtt
     1741 ataatacgat tgctgaagct aaaaaaaatt ttgataaaac tattgattta attctgcttg
     1801 atttaaatct acctgatggt attggatacg agttttgcca atatgttaaa agtatagaag
     1861 atactccagt gatatttttg acagtccgtg atgatgaaaa agacattata cgtggattag
     1921 atatgggtgc agatgactat gtggttaaac cgtttttgct ctcagtactt caatccagaa
     1981 ttaatgcagt tcttagaaga actcattcaa atactaacaa tactattaca tgtgaaaata
     2041 taagcttgaa taaggttgaa gtcaaagcat atttagataa tgttgaagta cctttgacag
     2101 caggagaata taaacttctt ctaagtctta tggaaaataa aaatagagct atgactcgta
     2161 caaagttact agaaatgctc tgggatgtag atggtaattt cgtaaatgat aacactctta
     2221 ctgttacaat aaaacgtctt agagaaaaat taaaaaatcc accttgtata aaaacattaa
     2281 gaggcatagg atatagaatg gaggacaact agtgaaaact ccaagaaata tcaacagtac
     2341 agttgttttt ataatattct gcttaattgt aactttttta ttgagtgtaa ttctaacaac
     2401 tataacttct tttaatgaat acaatgtact gtctaaggct cttggtgcat catatgagta
     2461 caatcctaag tctacagaaa gtatactaaa atcactgaaa aattctagtg ataaggattt
     2521 taaaacagga aatgaaattt tatcaaaaca tggatattct aaaagtacat tttggagtaa
     2581 aaactttttt tattttctag tgatatcgtt tggtataatt tcaacaatgg ctacagttgt
     2641 atatataatc aaacatagaa caaataagca aaaactgagt agaataaaca atcttactca
     2701 atacttagaa gaagtgaatt taggaaaaga gtctgtactt ttacgatgtg aagatgaatt
     2761 ttctcacctt gaagatgaga tatataaaac agttggggaa cttcgttgtt caaaagaatt
     2821 tgctttaaaa gaaagacaaa ctctttcaga taatcttgca gatattgctc atcaacttaa
     2881 gactccaatt acttctatgt cacttatggc acaattactt tcagaaaatt gtccagatga
     2941 agaaatagat tatattaaca gacttacaaa tcaaatctca agacttgaga gactagtttc
     3001 atctttactt acattatcaa aattggatgc aagtacttta atatttgaaa gcaaacttat
     3061 agatgtacat tctcttctta cttatgcaat tgagcctata gaaggttctt taagagaaaa
     3121 acatcagaca tttactatcg aggcttctcc ttcggtagaa tttgaaatcg atattaattg
     3181 gactttagaa gctcttttaa atattcttaa aaattgtagt gagcatatag aagatggtgg
     3241 atatatagtt gcttattata gtcaaaatcc tctatatgtt gagattatca tagaagataa
     3301 tggtaagggg tttgctcagg aagaacttcc atatattttt aatagatttt atcgtggtaa
     3361 aaatgctagt aaggacagta ttggtatagg gcttgctcta tcaaagtcta ttatagaacg
     3421 tcaaaatggt actatacatg cagaaaattg ccataatggt ggtgcaagat tcataattaa
     3481 attttacaca taaaatgctg gattataggt aaaataccta taatccagct atatctatat
     3541 tctatgttat ctattgtttt agtaatatac ttctatttac atttaaggtt ttattaaaca
     3601 aacaacttaa ctatccctat acttgtaagt agtcttaaat cgaaaccttt aaataagttc
     3661 aaatagattt tgtttatatt gtttcatttt ttatagattc tactatattt tcattcttca
     3721 actgcttgta tcctgtcata tatgatgcta ttacaataaa aagtatcaat atagtaaaac
     3781 ctattattgt agatataggt gcaaatggta aatactctat aaaatatatt tcatttatac
     3841 gtagaaaagc atacactatt cctatttgca caggaatact aagtagtaat ggtgtaattc
     3901 ctaagaataa accttccaaa ataagcatct tttttatgcc ttctggagat aatccaacag
     3961 aacgcaacat agcaaactct tgtcgtctac ttctaatatt tcctgacact gtagcaagta
     4021 catttgataa tccaatcata gccaataaac ctgtaataaa gcctactatt aaattcatgg
     4081 tctttattcc actagcatct tgtttttcac tttctaaaac atcctcaata ctataatctc
     4141 ctgaaccata gtctttatta taaatggact ctaatttttc tcttactcta ggaatgtcct
     4201 cacgattatt tactttaaat actcctatca gcctatttga acgtaaccta cttacttcat
     4261 cataattttt agatatttcc tgcaccatag acattggaac tatttgcatt aatgtatact
     4321 ttgaactagt atctactatt ttaggaagtg tgcttgtaat agctcccact ttcatgttgt
     4381 aagtataatc ccctttatct tcgtcatata ctttttcatt taatttaatg ttatctccta
     4441 ttgaaagatt taaataatct atatatactt tatttctacg tgtactacga ttaatatctt
     4501 cctccttatt atatacaatt gaggttggat tttttgtatc ataaaataac tttgggtcta
     4561 cagaaatttg cctacaatat tctgtaaaac tcttatcatc aagcccaatt atatttgaaa
     4621 ttatacgaaa ctttccatca cgttctatag gagagtactt tttagatgaa gctatatttt
     4681 taaatcctcc atttttcgca agttcttttg aagcatcttt ttcagtcaac caagtggaag
     4741 cagacatttt ttgataatat agtgaacttt ttacaccttc tgtattatct aatttttcaa
     4801 tgaaagcaga ctctacaggt tcactatttt ccaaataaac tgctatatct ctttggtcct
     4861 tataattatc acttccataa acagcttttg ctccttcttg attggcaatt atatgcaaaa
     4921 aacatgtaag taataaaaaa gataatgtta aagatattgt tgcagtacga taagattttt
     4981 tacgagcacg tagtgcgttt gatgcaagtt ctccttctat tccaaatatc tttcctaatg
     5041 aatatttatg cgactttttg attttaacac tatctccttg tcgtattgct tctataggag
     5101 atatctttga tacttttcgt gctggtataa gtgcagaaat ccagactgtt actattgtaa
     5161 gtaaaactgc tggcagaaaa gcaggaatac cataagtaaa taccacttct ggaacatcag
     5221 tatgataatt aactgaatta acataaacta taagcctatt acacaataac catcctaaaa
     5281 ataaacctat tggtaatggt ataattgtca gtaaaaagcc ttcaaacact acagaccttt
     5341 taatttgttt tggtgatgca ccaacactag ataatatccc taactgagta agtctagcat
     5401 ttgcagacag tgaaaacgcg ttgtaaatca ccattacaaa cacggctact gtaaaaattg
     5461 ctattgtaag atacataata ggagttgaaa attttgatat actactcata gatgctttct
     5521 gttctggtga aaagactaac atctttaaaa gataattact attgtattta agattgtatt
     5581 ttccatattc attagtcttc catcccaatg atttagcaag ttttggaagt tccttatacg
     5641 tgtctcttat atttttaaat cgcaagtaca cagttatact atcattaggt tttatacttg
     5701 ttttatcaag atatccaaga gctgtatatg caggtattac tgatgaagta gaaacatcta
     5761 cctttcctac tattgtcaat gtagcctctg aaattttttt aaaactttcc cctttcgatt
     5821 ttggggctat gggttcaaga ggctttccat ttaggattct atctcctatt gggagagtaa
     5881 ttttatcccc aatttttaca cttggattat tctcaaaata ttgttttgat aatgcaattt
     5941 cattagcatt atttggtcga cgaccctcta ttatagtatc cttttctggc atagaattcc
     6001 aatactctga tgtagcattt ctataaatta tataatctct tttctcatca ttaattttgc
     6061 ctacatacca ttgtcctttt accattacat catcaactga agaaaaattt tcaattaatg
     6121 gtaagtcact tccataagta tagtcaaaca attcaccatg ccagttacct tctttctcaa
     6181 ttgaaagttt tataaaatct gtccacatgg tatacaacag tccacacaaa gaagacatca
     6241 tggttgtagt aagtaatata gctaacatta tagctatact actacgttta ttgcgtcgta
     6301 caaagtctaa ggtgtattct tgaagaatat tcatttcttc accacctttt cagagacaat
     6361 cactccatct tccatagtta caatacgatt tgcctctagt gctattttct catcatgagt
     6421 aataagaagt attgtctgat taaatcgttt atttgataag tttaacaaat ctacaatttc
     6481 ttctgaattt tttctatcga gattaccagt tggctcatct gctaataaaa tagctggtct
     6541 ataaattaaa cttctagcta tagataccct ttgttgttgt ccacctgaaa gttgacttgg
     6601 aagatggttt aatctatcag ataatcccaa tatagataca atctcagaga atctatcttc
     6661 atccaccttt cttttatcaa gtaacatggg taataatata ttttttctaa catcaagagt
     6721 aggtataaga ttataaaatt gataaataag acctactttt ctacgtctaa aaactgcaag
     6781 agcttcttcc tttaaacttg ctatttctgt atcttcaaca taaatctttc cagatgtagg
     6841 tctatctact cccccaagca gatgtaacaa tgtagatttg ccagaaccag atgctcctac
     6901 aatggataca aaatcacctt tttgaactga taaatttaca ttattaaggg ctgtcactct
     6961 ggtttgattt gaaccatata tttttgtgag attttcacat tttaatattt ccatatacaa
     7021 tcatctcctt tatggttaaa ttttagcaac ctaaaatgac attatagtga catgaataca
     7081 aatataaatt actattaaaa atattgttta gtttttgcac attatttaaa attataatat
     7141 aaaacaaaga ctatgcaata tattttattt atacttttaa tatattacat agtctttaaa
     7201 aggtattaac tttaaatcta taggtattca atttaataat caatagcaag tatttta
//
LOCUS       DAELLV010000185         7221 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.185, whole genome shotgun sequence.
ACCESSION   DAELLV010000185 DAELLV010000000
VERSION     DAELLV010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7221
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.185"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1074
                     /locus_tag="KPD58_002898"
     CDS             <1..1074
                     /locus_tag="KPD58_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBG0266632.1"
                     /translation="LSKREKDFIQYGFLLFLICITTYLVLTTLDIKLIPKIIRLVNKT
                     YIFLGILTIIVYMIIESIVTHLIINAVQKTKVKFIGIKMATMGFYYNLVTPFASGSQP
                     MQIYALTKYDVSLSKSVAIVTNKTVVFQSTVTVFCGILILLNKSLLMEQVHSVKVLIT
                     FGMIMNVSMLLGGLLIVFSPKQVKMIAEVLINFLSKFKTFKFLDSKRDRINHYIDDYN
                     YSIKIFIKNVRILVLSILLTVLQLIAYFSIVYCVYKASNLRGVSYVHIMTLQVFLYMA
                     ISPIPTPGNVGANEVTFFTMFSNIIPKELLGYSVLLYSGFVYYFILIVSGLFTFGTHY
                     TLNSWKKEDKVNSNLDKVIEKLN"
     gene            1223..1384
                     /locus_tag="KPD58_002899"
     CDS             1223..1384
                     /locus_tag="KPD58_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HBG0266633.1"
                     /translation="MKQDKQNEKQSEISKIKQSNDNKLEIAQELHVSKNIGNMLGYRI
                     DKKKMNRPS"
     gene            1725..2993
                     /locus_tag="KPD58_002900"
     CDS             1725..2993
                     /locus_tag="KPD58_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBG0266634.1"
                     /translation="MEASIHTIASNNLLIIFAIVSITGIICSKLSEIIKVPDVVLYLL
                     VGILIGPSFLKFIDIRGFQIENQLILTFGSAFILYLGGKEISLKVLRNVKISVFLLAT
                     LGVVISAFIMQQVIGFTFGISAMTALLAGTIIASTDPATLVPIFNQVKIKDRVKQTVI
                     SESAFNDATGAILTSAVLAVILSNKFSLGENIYELGTMVIVGVLVGLITGILLLKLVN
                     DKPYGIFKDFAPIISVISVVVAYELSTKLGGSGYMSCFIVGIITGNKKNFKIWLSQKS
                     YDADFYVAETLGTICRMAIFIILGSQVNLAQLSKYFMPSVITVLVLMFIARPLCVLVC
                     TLVDREAKWSKSEILFMMWVRETGVIPAALCGIISAMKVPGYEVISSVVFMTILITLV
                     IQGSTTKLVAKKLGLLEEEVINISEKVSTF"
     gene            3188..3976
                     /locus_tag="KPD58_002901"
     CDS             3188..3976
                     /locus_tag="KPD58_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBG0266635.1"
                     /translation="MFTVMDIVQPDTVEEAYSILNKRKTNQVIGGSAFLRMGKKRIGT
                     GIELSKLNLDYIKEYEDYVEIGSMTTFRTLETSSIIKNNFGRIIEDSVKNIIGVQFRN
                     VVTVGATVFSKYGFSDLIVALLSLDTEVELYNIGRISLEEFLNRDYEKDLLIKIYIKK
                     TNKNASYKSLRNAKSDYPILNVSVSKHMEQFKLCVGARPQKATIAKQASEFLSNNEIN
                     EINIDKAVEIASEELTFGSNMRASREYRKAMSKVLLKRAIMEVI"
     gene            3976..4425
                     /locus_tag="KPD58_002902"
     CDS             3976..4425
                     /locus_tag="KPD58_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HBG0266636.1"
                     /translation="MLVKININGKKKSVDIEPEEYLVDTLRKVGNLSVKRGCDTGCCG
                     LCTVLIDKKPTLSCATLTLRALDKEITTIEGLENEVKEFSEVLVKEGAEQCGFCSPGF
                     ILTVLAMKDELENPTDDEIKHYLTGNLCRCTGYMGQLRAIKTYLEVK"
     gene            4429..6693
                     /locus_tag="KPD58_002903"
     CDS             4429..6693
                     /locus_tag="KPD58_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG0266637.1"
                     /translation="MNIVGKGIKKIDGMSITTGKPVYTDDLSAKDALVVKILRSPHAY
                     AKIKDIDTSKAEMVDGVECVLTYKDVPKSRFTLAGQTYPEPSPYDRLILEDVVRYVGD
                     EVAIVAAIDEKTAIKAMNLIKVKYEVLTPVLDFEEAIGNKTMVHDEDRHCNFDIGMQR
                     EKNIVSHHDYTKGDVEKVLSNCDVVIEETYYTQAQAHAMMETYRAYNYLDHMGRLVVV
                     SSTQIPFHVKRHLSRALEIPASKIRVIKPRIGGGFGGKQTACVEIFSAIVTLKTGKPA
                     KIIYDRKETFNCSTSRHAMRLKVRLGATNDGIIKAIDIKALSDTGAYGEHASTTIGLV
                     GEKTLPMYNKVEATRFIADVVYTNKMPAGALRGYGASQGCFAVESTINMLANKLKMDP
                     TDLRLKNVVTEGETTFAYGKTLNSVDLIKCINKGKELIGWNEKYPCKHLDNGKIRSVG
                     MAITMQGSGIQGLDKSSAEIKLNDCGDYTLMIGSTDMGTGSDTILAQMAAEVLDTSIE
                     NITVISADTDLVPYDPGSYASSTTYVTGMAVVKAAQDLREKIISAGATKLGVEKDEVE
                     FDGIKLTSKKGSTLLFDIAVDSTIGPDTNQLIGLASHGSPVSPPPFIAGFAETEIDTE
                     TGKVDLVNYVAVVDCGTVINKNLAKIQVEGGIVQGIGMSLFEEVRYTEKGSMDTNTFM
                     QYKIPARCDIGNVKVDFVETYEPTGPFGAKSVGEVVINTPLPAIQEAVFNGIGVRINT
                     LPITPEKVFMEMNK"
     gene            6833..7144
                     /locus_tag="KPD58_002904"
     CDS             6833..7144
                     /locus_tag="KPD58_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266638.1"
                     /translation="MAGNLNNMRAVNNFRGDKNILECLVSFEGRSISQRKVRVFFKEK
                     QNQIEIDFAEEEISKLVENVVLNTSYQEMLYDEIEKQLEIDCIGTWMILSKLKDGSRV
                     H"
BASE COUNT         2794 a          750 c         1308 g         2369 t
ORIGIN      
        1 ttatccaaaa gagaaaaaga ttttattcaa tatggatttt tattattttt gatatgtatt
       61 actacttatt tagtattaac tactttagat ataaaactaa tacctaaaat tataaggcta
      121 gtaaataaga cttatatatt tcttggcata ttgacaatta tagtatatat gataattgaa
      181 tctattgtaa ctcatttgat aataaatgca gttcaaaaaa caaaagtaaa atttattggt
      241 attaaaatgg ctacaatggg attttactat aacttagtta ctccatttgc atctgggagt
      301 caaccaatgc aaatatatgc acttacaaaa tatgatgtaa gccttagtaa atcagtggct
      361 atagttacta ataaaactgt agtatttcaa agtacagtta ctgtattttg tggaatttta
      421 attttgctaa ataagtcatt actgatggag caagtacatt ctgtgaaagt cttgataact
      481 tttggaatga ttatgaatgt aagtatgttg ttaggtggac tactaatagt atttagtcca
      541 aaacaagtaa aaatgatagc agaagttttg attaattttt tatctaaatt taaaacattt
      601 aaatttttag attcaaaacg tgatagaata aatcattata ttgatgatta taattattct
      661 ataaaaatat ttattaaaaa tgtaagaatc ttagttttaa gtatattact tactgtgtta
      721 caattaatcg catatttcag tatagtttat tgtgtgtata aagcctcaaa tttaagagga
      781 gtttcatatg tacatataat gacattacag gtctttttat atatggctat atcaccaatt
      841 ccaacaccag gaaatgttgg agctaatgag gttacatttt ttactatgtt ctccaatata
      901 attccaaaag aattattggg gtactctgta ctcttatata gtgggtttgt atactatttt
      961 atattaatag tttcaggttt atttacattt ggaacgcatt atacattaaa ttcttggaaa
     1021 aaggaagata aagtaaattc taatttagat aaggttattg aaaaattaaa ttaataaaat
     1081 aattagggaa attctatata taaatatggt gttgaataag ctatattata ttgagatatt
     1141 tccctttttt gattaaaatt atagaaattt gaaataataa gtaataaaca gaaaaagtga
     1201 tattgttttg aaaggagaaa ctatgaaaca agataaacaa aatgaaaaac aaagtgagat
     1261 ttctaagata aaacaaagca atgataataa acttgaaata gctcaagaat tacatgtaag
     1321 taaaaatata gggaatatgc ttggatatag aatagataag aaaaaaatga atagaccaag
     1381 ttaatgaaaa tgttgaaaat ggtacatttt aaaaaatata tataatgaaa tgttttattg
     1441 ttaaatttaa acttatttta atggaggaaa aagtcgaaaa atatctaaaa agatatggat
     1501 ataaaaaata ttaatatata tatactataa acttgacaaa atcaaccttt taataactag
     1561 caaaaaatag caaaattata taagcgttat ttaattgtca aaaatgaaag ctaaattgca
     1621 tgtcctaaaa aaaatgattg ataacattta tttgtgtgct ataattatat agtaaaacaa
     1681 taagtatatt gcataatgta tgcaaatcgg gaggagagac ggacatggaa gcatctatac
     1741 acacaattgc cagcaataat ttattgataa tttttgctat tgtaagtata actggaataa
     1801 tttgtagtaa gcttagtgaa ataataaagg ttccagatgt agtgttatat ttacttgtag
     1861 gtattttaat aggaccatca tttttaaaat ttatagatat tcgtggattt caaatagaaa
     1921 atcaactaat acttacattt ggttctgcat ttattttata tttaggtgga aaagaaataa
     1981 gtttaaaagt attgagaaat gtgaagatat ctgtattctt attagctact ttaggagttg
     2041 tgatatctgc ttttataatg caacaagtaa taggatttac ttttgggata agtgcaatga
     2101 ctgctttact agcaggaact ataatagcat caacagaccc agccactctt gtacctatat
     2161 ttaatcaggt aaagataaag gatagggtaa aacaaactgt aataagtgaa tcagctttta
     2221 atgatgcaac aggagctata ctaacatcag cagtgttggc tgtaattttg tcaaataaat
     2281 tttcattagg tgaaaatatt tatgaactgg ggacaatggt tattgtcgga gttttagttg
     2341 gtttaattac aggaatactg cttttgaaat tagttaatga taaaccttat ggaatattta
     2401 aagattttgc accaataata tctgttattt cagttgtagt tgcatatgaa ttatctacaa
     2461 aattaggtgg aagtggatat atgtcttgct ttattgtagg tataataact ggtaacaaga
     2521 aaaactttaa aatttggtta agtcaaaaat cttatgatgc agatttttat gttgctgaaa
     2581 ctttaggtac tatttgtcgt atggcaatat ttataatatt gggtagccaa gttaatttgg
     2641 ctcagttaag taaatatttt atgccatctg taattacagt attagtatta atgtttatag
     2701 caagaccact ttgcgtttta gtttgtacac ttgtagacag ggaagcaaaa tggagtaaaa
     2761 gtgaaatatt atttatgatg tgggtaaggg aaacaggcgt aataccagca gcactttgtg
     2821 gtattatttc agcaatgaaa gttcctggat acgaagtgat ttcatctgta gtattcatga
     2881 ctatactaat tacattagta atacaaggaa gtactacaaa attagtagct aaaaaacttg
     2941 gattattaga agaagaagtg ataaatataa gtgaaaaagt atcaactttt taaagttttg
     3001 ctttaaaata gtacctgtta atggctattt taatattgtc taatgatgat tattgaaata
     3061 gtcatttttt tatgtataag taatttacag aatttacata ttagacatat ttattatcaa
     3121 aatttgttga agctatgata taattagttg tatttaaaat aagaatagca aataagggag
     3181 gaaatttatg tttacggtta tggatattgt acagccagat acagtagaag aagcgtactc
     3241 aattttaaat aaaagaaaaa ctaaccaagt aattggtgga agtgcttttt taagaatggg
     3301 gaaaaaaaga ataggaacag gaatagaatt atctaaatta aatttagatt atataaaaga
     3361 gtatgaagat tacgttgaga ttggttctat gactactttt agaacattag aaacaagttc
     3421 cataataaaa aataattttg gaagaataat agaagattca gttaaaaata taataggagt
     3481 tcagtttaga aatgtagtaa cagttggtgc aacagtattt tctaaatatg gtttttctga
     3541 cttgatagta gcattacttt cattagatac agaagtagaa ttatataata ttgggagaat
     3601 tagtcttgaa gagtttctaa atagagatta tgagaaagat ttacttatta agatatacat
     3661 aaagaagaca aataagaatg cttcttataa atcacttaga aatgcaaaga gtgattatcc
     3721 aattctaaat gtatctgtat caaaacatat ggaacaattt aagttatgtg taggagcaag
     3781 accgcagaaa gctacgattg caaaacaagc tagtgaattt ctatctaaca atgaaatcaa
     3841 tgaaattaat atagataaag cagtagaaat agcatcagaa gaactaactt ttggttctaa
     3901 catgagagca tctagggagt atagaaaagc tatgagtaaa gtacttttaa aaagagctat
     3961 aatggaggtt atttaatgtt agtaaagatt aatataaatg ggaagaaaaa aagtgttgat
     4021 atagaaccag aagaatatct ggttgatact ttaagaaaag ttggtaattt aagtgtgaaa
     4081 agaggttgtg acacaggctg ttgtggttta tgtacagttt taattgacaa gaaacctaca
     4141 ttatcctgtg caacactgac attgagagct ttagataaag aaataactac aattgaaggt
     4201 ttagaaaatg aagttaaaga atttagtgaa gttttggtaa aagaaggagc tgagcaatgt
     4261 ggtttctgtt ctcctggctt tatacttact gtacttgcaa tgaaggatga gcttgaaaat
     4321 cctacagatg atgaaataaa gcattatctt acaggaaatc tatgtagatg tacagggtat
     4381 atgggacagt taagagctat caaaacttat ctggaggtaa aatagaacat gaatatagtt
     4441 ggaaaaggaa tcaagaaaat agatggaatg agtatcacaa caggaaagcc tgtatataca
     4501 gatgatttat cagcaaaaga tgctttggtg gtaaagatac ttagaagtcc tcacgcttat
     4561 gcaaaaataa aagatataga tacatcaaag gcagaaatgg ttgatggtgt tgaatgtgtt
     4621 ttaacatata aagatgtacc aaaatctcga tttacacttg ctggtcaaac atatccagag
     4681 ccatctccat atgatagatt aattcttgaa gatgttgtaa gatatgtagg tgacgaagta
     4741 gctatagtgg cagctataga tgaaaaaact gcaattaaag caatgaatct tatcaaagta
     4801 aagtatgaag tacttacacc tgttttggat tttgaagagg caataggaaa taaaactatg
     4861 gtacatgatg aagatagaca ttgtaacttt gatataggta tgcaaagaga aaaaaatata
     4921 gtatcccatc atgattatac aaaaggtgat gtagaaaaag tattaagtaa ttgtgatgta
     4981 gtgattgaag aaacttatta tactcaagct caagcacatg caatgatgga gacgtatagg
     5041 gcatacaatt atttagacca tatgggaaga ttggttgtag taagttcaac tcaaatacca
     5101 tttcatgtaa aaagacattt atctcgtgca ctagaaatac cagctagcaa aattagggtt
     5161 ataaaaccta gaattggtgg cggatttggt ggtaaacaaa ctgcatgtgt tgagattttt
     5221 tctgcaattg ttactttaaa aacagggaaa ccagctaaaa taatttatga tagaaaagaa
     5281 acttttaatt gttcaacaag tagacatgca atgagattaa aagttagact aggtgctaca
     5341 aatgatggca taataaaggc aatagatata aaagcacttt ctgatacagg agcatatgga
     5401 gagcatgctt ctacaactat tggtttggtt ggagaaaaaa ctttaccaat gtataataaa
     5461 gttgaagcaa caagatttat tgcagatgtg gtatatacta ataagatgcc agcaggagca
     5521 cttagagggt atggagcatc acaagggtgt tttgctgttg aatcaactat aaatatgctt
     5581 gctaataaat taaaaatgga cccaacagat ttaaggttaa aaaatgtggt aacagaaggt
     5641 gaaactacat ttgcatatgg taaaacttta aatagtgtcg atttaataaa atgtataaat
     5701 aaaggcaaag aacttattgg atggaatgaa aaatatcctt gtaaacatct tgataatgga
     5761 aaaattagaa gtgtgggtat ggcaattact atgcaaggtt ctggtataca aggtcttgat
     5821 aagtcttcag ctgaaataaa attgaatgac tgtggagatt atactcttat gataggttct
     5881 actgatatgg gtacaggaag tgatacgatt ttagctcaaa tggctgctga ggttcttgat
     5941 acaagcattg aaaatataac agttatatca gctgatactg acttagttcc atatgaccca
     6001 ggttcatatg cttcaagtac tacttatgta acaggtatgg cagtagttaa ggcagctcaa
     6061 gacttaagag aaaaaataat ttctgctgga gcaactaaac taggtgttga gaaggatgaa
     6121 gtagaatttg atggtataaa attaactagt aaaaaaggta gcactttatt attcgatata
     6181 gcagttgatt ctacaattgg tccagatact aatcaattaa taggtcttgc ttctcatggt
     6241 agcccagtat ctcctccacc ttttatagca ggttttgcag aaactgaaat tgacacagaa
     6301 actggaaaag ttgatttagt aaattatgta gctgtagtag attgtggaac ggttataaat
     6361 aaaaatctag ctaagataca agttgaaggt ggaattgttc aagggattgg aatgtcatta
     6421 tttgaagaag ttagatacac agaaaaagga agcatggaca caaatacatt tatgcaatac
     6481 aagattccag ctagatgtga tattggaaat gttaaagtag attttgttga aacttatgaa
     6541 ccaactgggc catttggtgc taaatctgtt ggagaagttg ttataaatac acctttacca
     6601 gcaatacaag aagctgtgtt taatggaatt ggtgtaagaa taaatacact ccctattaca
     6661 cctgaaaaag tttttatgga aatgaataaa taattattta tgaaaattta gactagtttt
     6721 acatgttaaa caaccagtta tgtcatacct gttgaaagat atttaataag tagttattat
     6781 aatattatga attttactta taaaatacaa aattaaataa ggggatgatg ttttggctgg
     6841 taatctaaat aatatgagag cagtaaataa ttttagagga gataagaaca ttttagaatg
     6901 tttagtcagc tttgagggtc gttcaataag tcagagaaaa gtaagggtat tttttaaaga
     6961 aaaacaaaat caaatagaaa ttgattttgc agaagaggaa atttctaaat tggttgaaaa
     7021 tgttgtttta aatacatcat atcaagaaat gttatatgat gaaatagaga aacaactgga
     7081 aattgattgt ataggtactt ggatgatatt atctaaatta aaagatggta gtagagttca
     7141 ctgattttaa aataaattat tagagcctaa ttttaaggct cttttttgct gtttttaatt
     7201 aacaatagaa aatttatctt t
//
LOCUS       DAELLV010000186         7193 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.186, whole genome shotgun sequence.
ACCESSION   DAELLV010000186 DAELLV010000000
VERSION     DAELLV010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7193
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.186"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            118..1008
                     /locus_tag="KPD58_002905"
     CDS             118..1008
                     /locus_tag="KPD58_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG0266639.1"
                     /translation="MFEELKTFVAVVEYKNFTKAGEYLNLSQPSVSKHIKNLENYFKV
                     VLINRSIKQKTIFITESGQILYKRAKEILNLLNITYHDVSQVSDAITGHLKIGASLTI
                     GEYILPNFLALFSKKYPDIDVEVFIKNTSIVSSHVKDYILDIGLIEGTCSSPSFIQEY
                     FFEDKMVLALPYKSHLLKDFSFDKLQNQKWIVREDGSGTRDYLDMFLSVKEIIPKSMM
                     VFGSNYAVKESVRNNLGITIVSNLVTSLPVLNNELSVIELGSSYNRHFSYIFPKDITL
                     SKAATIFIEELKIFSNLNSI"
     gene            complement(1060..1986)
                     /locus_tag="KPD58_002906"
     CDS             complement(1060..1986)
                     /locus_tag="KPD58_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HBG0266640.1"
                     /translation="MKNQLNENHKKVVFFGVGAVGATFAEQFFNSKYDFKILCDNERK
                     KRYLEEGFIINGKRYDFDYVTKDEYKQEADFIIIGLKYNNLKENIKELDGLVGKNTVI
                     MSLLNGVDSEEIIGERFGIEKMVYSYVTNIDAKKINNNIIHTTNGIIVFGNKDNSEDR
                     KTNIITEVFDDVNIEYTLSKDIQRDMWWKYMVNIGVNQTSAILGAPYGVFQSSEHLRE
                     LAKSAMREVVAIAQAKNISLTEDDVEHSLHRILEHSKEGRTSMLQDVEAHRLTEVDMF
                     SKNICKLGKKYNIPTPINQTFFYMIKVIESRF"
     gene            complement(2201..2785)
                     /locus_tag="KPD58_002907"
     CDS             complement(2201..2785)
                     /locus_tag="KPD58_002907"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBG0266641.1"
                     /translation="MSKVDVVDILKKSDALLEGHFLLSSGKHSNRYVQCAKVLRFPEY
                     AAEVLSTVVEQIKDLDIDLVVGPAMGGVIVSYELGRQLGKEAVFTERKDNTMELRRGF
                     EVKKGAKIIIAEDVVTTGKSTMETKRVLEALGGEVVGVACIADRTNHDIGMPIYSAIK
                     LDIQVYESDECPLCKEGKLPVVKPGSREFKELGM"
     gene            complement(2870..3772)
                     /locus_tag="KPD58_002908"
     CDS             complement(2870..3772)
                     /locus_tag="KPD58_002908"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBG0266642.1"
                     /translation="MGNLSVKFGSVEFKNPVIMASGTFGFGKEYNEIYDIQKLGGISS
                     KGLTLNKKPGNNGMRVHETPSGMMNSVGLENPGVQGFIDYELPFFSKLDLVRIANVGG
                     GTLEDYLLGVQMLNDKPIDIIELNISCPNVKAGGMAFGIKNEVAREVVREVRNITKLP
                     LVIKLSPNAEDIVGMAKVCEEEGADGVSLVNTFKAMAIDIKNRRPVFENVYAGLSGPA
                     IKPIALRMVHEVCKNVNIPVMGMGGITKATDAIEFIMAGATCIQVGTANFMNPRIGIE
                     IIDGINEFMDREGIKSLDEIRGII"
     gene            complement(3765..4457)
                     /locus_tag="KPD58_002909"
     CDS             complement(3765..4457)
                     /locus_tag="KPD58_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HBG0266643.1"
                     /translation="MYKILENIYIGEDMYRMKVKGNFEGKMGQFYMLRAWDTYPVLSR
                     PISIHDIDEEGITFLYKVVGEGTQILSNLKVNDNIKLEGPYGNGYAKVDGKVALVGGG
                     IGVAPLYLVAKNIKNCDAYLGFREDVILEDEYKQVCNKVYTTVGNTFVTDIIDVEKYD
                     YILTCGPTPMMEKLVKMVEGTKTRIMVSLENHMACGVGACLVCTCKTNGGNKKTCKDG
                     PVFWGEDVIFNG"
     gene            complement(4496..5416)
                     /gene="pyrB"
                     /locus_tag="KPD58_002910"
     CDS             complement(4496..5416)
                     /gene="pyrB"
                     /locus_tag="KPD58_002910"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HBG0266644.1"
                     /translation="MLKSRNLIQPEDFSIEEIDEILELAQKIIDNPSKYSRICEGKLL
                     ATLFYEPSTRTRLSFESAMNRLGGRVVGFSEPNSSSASKGETLGDTMRIVSGYVDIIA
                     MRHPQSGAASEAARYTEVPFINAGDGKNQHPTQTLTDLLTIKSLKGTLESHTIGLCGD
                     LKYGRTVHSLVKAMARYKNTKFVFIAPEELKMPDYIKEAIKGHAYYETNNLDDVIGSL
                     DVLYMTRVQQERFEDKSEYERLKNYYILNKAKLEKASKDMLVMHPLPRVNEIDIDVDS
                     DDRAVYFKQAKYGMYVRMALIIKLLGINED"
     gene            complement(5712..6734)
                     /locus_tag="KPD58_002911"
     CDS             complement(5712..6734)
                     /locus_tag="KPD58_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG0266645.1"
                     /translation="MKKKILIPVFASVMALSVSSIVNADEVNDSSQNKDDKTNTELNL
                     GEYKEVKYKVAKIKDGVAIKIREEGQVQNIAYSGDEFTVLGTQGEWVKVKVEDGEGWL
                     ATRYVDISEGVGYTNADKVNLRKDKSESSEVIEELEKGSSLLVLEDNGDWLKVKDGET
                     EGYVKSSYISDKAPVIEEPQVNPSIDQNVDSTNNNQQNNANTNQQNNNSNVPTANSNA
                     VQAVLNLAYSKQGCPYVWGAEGPNTFDCSGFTQYVYRNAVGKNIPRTSKAQSKYGQTV
                     SKANLQPGDLVFFTTNGSGSVSHVGIYVGGGNMIHSPSTGKTVSVTSINSSYYTARFV
                     TAKRIL"
BASE COUNT         2266 a         1311 c          782 g         2834 t
ORIGIN      
        1 aaataaatat ataatatcgt aatgattaat gtttttaatg gttattatat atagtataat
       61 tatctgtaga aagtatataa aaaatgtaaa atttcattag gtaaaggaga caaaaaattg
      121 tttgaagaat taaaaacatt tgtagctgtt gttgaataca aaaactttac taaagctggg
      181 gaatatttaa atctatcaca accaagtgta agtaaacaca taaaaaatct agaaaattat
      241 tttaaagttg ttcttataaa caggtccatt aaacaaaaaa ccatttttat aacagaaagt
      301 ggacaaatct tgtacaaaag agctaaagaa atattaaatc ttttaaatat tacttatcat
      361 gatgtaagcc aagtttcaga tgctataact ggtcacttaa agattggtgc aagtttaact
      421 attggagagt atattttacc aaattttcta gcattatttt ctaaaaagta tcctgacata
      481 gatgtagaag tttttataaa aaatacatca atcgtatctt ctcatgttaa ggattatatt
      541 ttagacattg gtcttattga aggcacttgc tcttcccctt catttattca agaatatttt
      601 tttgaagata agatggtttt agcacttcca tacaagagtc atttattaaa agattttagt
      661 tttgataaac ttcaaaacca aaaatggata gtcagggagg atggttctgg aaccagagat
      721 tatttagata tgtttttaag tgttaaagaa attattccaa aaagtatgat ggtttttgga
      781 agtaattatg ctgtcaaaga atctgttaga aataatttag gaattacaat agtctctaat
      841 ctagtgacaa gcttacctgt tctaaacaat gagctgtctg tcatagagct tggaagcagc
      901 tacaacagac atttctcata tatatttcca aaagatataa ctttatcaaa agcagcaact
      961 attttcattg aagagttaaa aatatttagt aacttaaata gtatataaca ttaataaaat
     1021 ttaaaatcaa taattatcaa tatttcttac aaagaccctt taaaatctac tttcaattac
     1081 ttttatcata taaaagaaag tctgatttat aggagtaggt atgttatatt tttttccaag
     1141 tttgcagata ttcttagaaa acatatctac ttctgtaagt ctatgagctt ccacatcttg
     1201 aagcattgat gttcttcctt cttttgaatg ttctagtatt ctatgtaatg aatgttccac
     1261 atcatcttct gtaagagata tgttttttgc ttgtgctata gcaacaactt ccctcattgc
     1321 agattttgct aattctctta aatgctcaga actctgaaaa actccataag gtgcaccaag
     1381 tatagctgaa gtttgattta caccaatatt aaccatgtac ttccaccaca tatctcgttg
     1441 aatatctttt gataaagtat attctatatt tacatcatca aagacttcgg ttattatatt
     1501 agtttttcta tcttcactat tatctttgtt accaaataca attatcccat tagtagtgtg
     1561 tataatatta ttattgattt tctttgcatc tatattggta acatatgaat ataccatttt
     1621 ttcaattcca aatctttctc ctattatctc ttcgctatca actccattta gcagagacat
     1681 tataactgta ttttttccaa ctaatccatc taattctttt atattttctt ttaaattatt
     1741 atattttaga cctataatta taaaatcagc ctcttgttta tactcatctt tagttacata
     1801 atcaaaatca tacctttttc catttattat aaatccttct tctaaatatc ttttctttct
     1861 ttcattatca caaagaattt taaaatcata tttagagtta aaaaattgtt ctgcaaaagt
     1921 agctcctaca gcacctactc caaaaaatac aaccttttta tgattttcat ttaattgatt
     1981 tttcatactt acccctccct aatatataat acttttacct tatacttata ttgtactgct
     2041 actatctctt attttgtaaa tatattttta acaaaaaaaa cagctaacct tcatttataa
     2101 ggctaactgc tttttatttt taagttatat gttatattac aaatttttac tctaaaaaat
     2161 ttacattttt taagtaacac tataaataat taagttttta ttacatccct aattccttga
     2221 actccctact tccaggctta acaactggta attttccttc cttacataaa ggacactcat
     2281 cagattcata aacttgaata tcaagtttta tagcactata tataggcata cctatatcat
     2341 gattagttct atctgctata catgcaacac ctacaacttc tccacctaag gcttctaata
     2401 ctctttttgt ctccatagtt gatttaccag tagttacaac gtcttcagca attattatct
     2461 ttgctccttt tttaacttca aatcctcttc ttaactccat tgtattgtct tttctctcag
     2521 taaatacagc ttcttttcct aattgtcttc ctaactcata agaaactatt actccaccca
     2581 tagctggtcc tactactaag tctatatcta agtcttttat ttgttcaaca actgtactta
     2641 atacctcagc agcatattct ggaaatctta atacttttgc acattgtacg tatctattac
     2701 tgtgttttcc tgaagataat aaaaaatgtc cctccaacaa tgcatcactt ttctttaata
     2761 tatccactac atctacttta ctcatattat ttttcctcca tgttaatgtt ataaaattat
     2821 tgtttattta atctatattt tttatcttta aaatactatt tattctacct tatataattc
     2881 ctcttatttc gtctaaactt tttattcctt ctctatccat aaattcatta attccatcta
     2941 ttatttcaat acctattcta ggattcataa aatttgcagt tccaacttgg atacaagtag
     3001 ctcctgccat tataaactct atagcatctg tggctttagt tattccgccc attcccatta
     3061 caggtatatt tacattttta cacacttcat gtaccattct caaagctata ggttttatag
     3121 ctggtccaga taacccagca tatacatttt caaatactgg tcttctattt tttatatcaa
     3181 ttgccattgc tttaaaagta ttaactaatg aaactccatc agcaccttct tcttcacaaa
     3241 cttttgccat tcctacaata tcttctgcat tgggtgataa ttttattact aaaggcagtt
     3301 tcgttatgtt tcttacctca cgaacaactt ctcttgccac ttcatttttt ataccaaaag
     3361 ccattccacc agctttgaca tttgggcaag atatatttaa ttcaataatg tctataggct
     3421 tgtcatttaa catttgaacc cctaataagt agtcttctaa tgtcccacct ccaacattag
     3481 ctattctgac taagtctagt ttactaaaaa aaggtaattc atagtctata aatccttgaa
     3541 ctccagggtt ttcaagccca acactattca tcataccgga tggtgtctca tgaactctca
     3601 taccattgtt accaggtttt ttatttaaag taagaccctt gctacttata ccaccaagtt
     3661 tttgtatatc ataaatctca ttatattctt ttccaaatcc aaaagttcca gacgccatta
     3721 ttactggatt tttaaattct acacttccaa atttcacact taaattaccc attaaaaatc
     3781 acgtcctctc cccagaacac tggaccatct ttacaagtct ttttatttcc tccattagtt
     3841 ttacacgtac atactaaaca ggcacctact ccacaagcca tatggttttc aagagatacc
     3901 ataattctcg ttttagtacc ttctaccatc ttaactaatt tttccatcat tggagttgga
     3961 ccacatgtta atatataatc atatttctca acatcaatta tatctgttac aaaagtattt
     4021 cctactgttg tatagacttt attacatact tgtttatatt catcttctaa tataacatct
     4081 tctctaaacc caagatatgc atcacaattt tttatatttt tagcaaccaa atatagtgga
     4141 gctacaccga ttcctcctcc tactagagct acctttccat caaccttagc atatccatta
     4201 ccataagggc cttccaattt tatattatca tttaccttta ggttactcaa aatttgagtt
     4261 ccttctccta ctactttata aagaaaagtt atcccttctt catctatatc atgtatactt
     4321 ataggtcttg aaagtactgg ataagtatcc catgctctta acatatagaa ttgtcccatt
     4381 ttaccttcaa aattaccttt tactttcatt ctatacatat cttcgcctat atatatattt
     4441 tccagaattt tatacatccc aaaatctcct ttgttttact attatatgat aactattagt
     4501 cttcatttat acctaaaagt tttattatta aagccattct aacatacatt ccatatttag
     4561 cttgtttaaa atatacagct ctatcatcac tatctacatc tatgtctatt tcatttactc
     4621 ttggtaatgg atgcattact aacatatctt tagatgcctt ttctaattta gctttattta
     4681 aaatataata gtttttaagt ctttcatact ctgatttatc ttcaaatctt tcttgttgaa
     4741 ctctagtcat atataaaaca tccaagcttc ctataacatc atccaaatta ttagtttcat
     4801 aatatgcatg tccctttata gcttctttta tataatctgg catttttaac tcttcaggcg
     4861 ctataaatac aaactttgta tttttatatc ttgccatagc cttaactaaa gaatgcacag
     4921 ttcttccata ttttaaatca ccacataatc ctattgtatg gctttctaaa gttcctttta
     4981 atgattttat agtaagtaag tctgtaagtg tttgagttgg atgctggttc ttgccgtctc
     5041 ctgcatttat aaatggcact tccgtatatc tagctgcttc tgaagctgca cctgattgag
     5101 gatgtctcat agctattata tcaacataac cagatactat cctcattgta tctcctagag
     5161 tttcaccttt tgatgcagag gatgaatttg gctctgaaaa gcctactaca cgtcctccta
     5221 atctgttcat agcagattca aagcttaatc ttgttcttgt tgatggttca taaaataatg
     5281 tagctaatag ttttccttca catatacgag aatattttga cggattgtca attatttttt
     5341 gagctaattc taatatttca tctatttctt ctattgaaaa gtcttctggt tggattaaat
     5401 ttcttgattt taacatcttt cgtcctcctt ttttagcctc tctgaactaa aattaaaagt
     5461 atttttgtac tgttagtagc ttaccacctt tgccctatcc tgtcaaggga ttgcacaaat
     5521 aatcatcata tgaaaaggct cctatttaac agtcatatta acttgaatct tgattagatt
     5581 taaagtgata caaaaacgct ctatagtaca tttaaaatat aatcattttt tgtttccatg
     5641 attaaattaa atataatata gagcgtttat atatatttat gtatttatca atttgtcata
     5701 ttacaaaccg attataatat tctttttgct gtaacaaatc ttgctgtata gtatgatgaa
     5761 tttatactag ttacactaac tgtttttcct gttgatggtg aatgtatcat attaccccca
     5821 cctacatata ttccaacatg actaacagaa ccagaaccat tagttgtgaa gaatactaag
     5881 tctcctggtt gtaaatttgc tttacttaca gtttgtccat atttagattg tgctttagat
     5941 gttcttggta tattctttcc tactgcattt ctatacacat attgtgtaaa tccagaacag
     6001 tcaaaagtgt ttggtccttc tgctccccaa acatatggac aaccttgctt agaatatgcc
     6061 aagttaagta cagcttgaac tgcattagag ttagctgttg gcacatttga attattattt
     6121 tgttgattag tatttgcatt gttttgttga ttattatttg tagaatctac attttggtca
     6181 atacttgggt ttacttgtgg ctcttctatt acaggagctt tatcagatat ataagatgat
     6241 tttacatatc cttctgtttc cccatcttta acttttaacc aatctccatt atcttccaaa
     6301 accaataaag aacttccttt ttccagctct tcaattactt ctgaactttc actcttatct
     6361 tttcttaagt ttactttatc agcgttagta tatcctacac cttcagatat atctacatat
     6421 ctagtagcta accaaccttc gccatcttca actttaacct taacccattc tccttgagtt
     6481 cctaaaaccg taaattcatc tccagaataa gctatgtttt gaacttgacc ttcttctctt
     6541 atctttatag ctactccatc ttttattttt gcaactttat attttacttc tttatattca
     6601 cctaaattta attcagtatt tgttttatca tccttatttt gagatgaatc atttacttca
     6661 tctgcattta caattgaact tactgataat gccataactg atgcaaatac tggtattaat
     6721 atctttttct tcacgataaa cctcctaaaa ttttcccata attatcttat gattctattt
     6781 tgtaggtgaa gtgtacctaa ggataaaata ctaatacaca atacattaat aaaagctcta
     6841 tctgtaactt ttttgtaaac cactttatat aacttttata gtatcattat attaataaaa
     6901 taaataaatt agcttatcca atatgtcacc taaatataca ttatttactt aattatataa
     6961 tatgggtctg taaaagtaaa gaattttgta ccattcgagg ttaaagtgcg cataaatatt
     7021 gttttatgtg tttttttatg cgcttttata gggcgtatat tttttgatgg atttagtata
     7081 aagttagata tgctgtgaaa tttttaaccc tacataaatc aaagcctatt tcctaatagt
     7141 gattctcaaa ttctaagtta aaaagatgaa aattttggat aatataaata tta
//
LOCUS       DAELLV010000187         7186 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.187, whole genome shotgun sequence.
ACCESSION   DAELLV010000187 DAELLV010000000
VERSION     DAELLV010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7186
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.187"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            467..976
                     /locus_tag="KPD58_002912"
     CDS             467..976
                     /locus_tag="KPD58_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009528606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBG0266646.1"
                     /translation="MSKELAINEQIKDKEIRVLSPTGEQLGVMPTKEAQAMANSKNLD
                     LVQISPNANPPVCKIMDYGKFRYEQARKEKEAKKKQKTIVVKEVRLRPGIEQNDLNTK
                     ANNAIKFLKKGDKVKVELRFRGRELGHKDIGKEVMLKFLDIIKEFGEPTKAPAFEGNN
                     MVVIIDPKK"
     gene            1004..1198
                     /gene="rpmI"
                     /locus_tag="KPD58_002913"
     CDS             1004..1198
                     /gene="rpmI"
                     /locus_tag="KPD58_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBG0266647.1"
                     /translation="MPKMKTHRGAAKRLKKTGTGKLKRAKAFKKHILTKKSAKTKMNL
                     RKSTLVSDGDAKRIAQLLPY"
     gene            1254..1610
                     /gene="rplT"
                     /locus_tag="KPD58_002914"
     CDS             1254..1610
                     /gene="rplT"
                     /locus_tag="KPD58_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBG0266648.1"
                     /translation="MARVKKAMNARKKHKKILKLAKGFRGSRSKLYRPANTFVMKALK
                     NAYIGRKLKKRDFRKLWIQRINAAARMNGISYSRLMNGLKLSGVEVNRKMLSEMAIQD
                     PEGFAKLAEVAKAKLA"
     gene            1920..2465
                     /locus_tag="KPD58_002915"
     CDS             1920..2465
                     /locus_tag="KPD58_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266649.1"
                     /translation="MNEKLMDCFVNPIKCKLLIEIFSNNRTTAKQLGEKFQDISKATL
                     YRYLNKMLKDDIIKVVEENKIRGTVEKVYAMKLDIADDVNKIIDENSGEEYAQLFTQY
                     MMGFLREFYEYAERKEKDILKDGSGFSVCPVYASIDEIVEVSKKIASILEPLRNNSHE
                     EGRRMHSIGVIITPPKKDEGK"
     gene            2900..4825
                     /locus_tag="KPD58_002916"
     CDS             2900..4825
                     /locus_tag="KPD58_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG0266650.1"
                     /translation="MKIKFVIMPLVIFLMVLFVGCQSTKDTEVKAKDTEIKILGTGDL
                     HSILTDSMVSYVNEEREKNKNLLMVDAGDFYGTQSREMWEWSSGKKLINIKRNGRAEY
                     DDIIKSSKDEVPIVKDMAKLKYDAVVLGDNEFVSNDKQSLDKLVSDFKNNNMPLVSAN
                     IYEQSGENYVQPYVMKNIKTDEGNVKVGILGLTIKEVGETLGLEDFEKDKKARELGEQ
                     ADYKGKLYANDLVEDAKKWIKVMEKESPDIIVAVVHSGEEPKTDRNPGNRIKELATTV
                     DGIDAIVAGHTHEKIDEHKYKNKSGDEVIVTQPGWHGDRVSEINFKLNKENGKWDIKE
                     KSSKLKIIDKEVSIVATADLHSHFSDKLTVDLVNERSNPIEPVVVDAGDFLDPQTDEM
                     TQWYKEWKSIRDNNSDKTISRCPMVMDMNQGMYDAVVLGNHEFVSEKDEFWSDERLLD
                     AVIEDFEQVAISVLSANIYRQSGKNYVKPYIIKDVETSEGNVKVGILGLTIKEVGKGL
                     KNVNLQEQFQYKDKLYANDLVEDAKKWVKVMKKKKPDIIVAVVHSGEEPKNPKHPGNR
                     IKELATTVDGIDAIVASHTHEKIDEHEYKNKSGEKVIVTQPGEHGKYYSKITFKVNKE
                     KGSWTINDKFSKTIEVE"
     gene            5226..7130
                     /locus_tag="KPD58_002917"
     CDS             5226..7130
                     /locus_tag="KPD58_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG0266651.1"
                     /translation="MKIKFIIVASIIFLIVLLVGCQSTKDTEINVLATSDLHSIIPDN
                     LISYVEEERKYDENLLMVDAGDFFDMQSGEMNKWFTGQKLVRFKDGIPEYKKIAEPRE
                     GEVPLAKKMAKLKYDAVTFGNHEFVSNDKQSLDKLVSDFKNNNIPLVSANVYEQSGEN
                     YVKPYVMKNINTDKGTVKVGILGLTIKEVGERSRFKSFEQDKKARELGEQPQYKGKLY
                     ANDLVEDANKWVNVMEKKEKPDIIVAIVHSGEEPKNPKNPGNRIKELATTVEGIDAIV
                     AGHTHEKIEQHTYKNNSGEEVIVTQPGAHGNSISKINFKLDKKNDKWFIKDKYSELKV
                     FGKEVNIITTSGLNPKFSDEMAVNLFNERDRDVQPIFIDTGDFLNTNTKEMTKWAKEW
                     QYNENKGIYKKINEWPIVYLGYDAVVLGSHEFTEDKETFEEKKSTLDYIIENFEEMKI
                     PVLSANIYDESGENYVKPYIIHNVETREGNIKVGVLGLTTTDKDEEDLSNSSLSKDSK
                     TSKIPEETGKLYTNNLVKDASEWVKVMQKESPDVIVAVVHSDNNSTLKNPSSEVKKLA
                     MSVDGIDAIVVGHTKNKVEEHIYKNKSGDEVIVTQSGEDCYSKISLELRKENGKWNII
                     NKYSKAIKMK"
BASE COUNT         3026 a          717 c         1356 g         2087 t
ORIGIN      
        1 tagtatatta gagtacaata tagaatttgt tatgtagtat agtagtatat tagaatacaa
       61 tatagaattt gttgggtagt atagtgttat attagaatac aatataggat ttgttatgta
      121 gaatatatag agtcatgtag aatatacata tataaaagga ttttgggtat aaatttaaaa
      181 attaatagca aaatgagtag tatagacata taattagtag taggaagtaa ttaaattttt
      241 cctaaacaaa aaaaatagtt gcaaataatt tgattaagtg atatagtaaa taaggtaaga
      301 ataaaacaaa gaagaagtcc gcttctcacc ttacgccgga ggttagtaag gtaaatataa
      361 gttaaagctt aattttaatt taagtttgtt taaatttggt aacttatatt gcggatgatt
      421 acgtcatccg ctttttattt tataaaaatt ttggaggtgt cctacaatta gcaaagaatt
      481 agcaatcaat gaacaaataa aagataagga aataagagtc ctttcaccaa caggtgaaca
      541 acttggagta atgcctacta aagaagctca agcaatggct aatagcaaaa acttagattt
      601 ggttcaaatc tcgccgaatg caaatccacc agtatgtaag ataatggatt atggaaagtt
      661 tagatatgag caagcgagaa aagagaaaga agctaagaaa aaacaaaaaa ctatagttgt
      721 aaaagaagta agacttagac caggtattga acaaaatgat ttaaatacga aagctaataa
      781 tgctataaag ttccttaaaa aaggggataa agtcaaagtt gaacttagat ttagaggaag
      841 agaattagga cataaagaca taggtaaaga agttatgctt aagtttttag atataataaa
      901 agagtttgga gaaccaacaa aagcccctgc atttgagggt aataatatgg ttgtaattat
      961 agatccaaaa aaatagatga ttaactgaga ggaggaattt gtcatgccta aaatgaaaac
     1021 tcatagaggt gcagcaaaga gattaaagaa aacaggaact ggaaagttaa agagagctaa
     1081 agcttttaaa aaacatatat taactaaaaa atctgcaaaa actaaaatga acttaagaaa
     1141 atcaacttta gtaagtgatg gagatgctaa gagaatagca caattattac catactaaga
     1201 tacagattgt aaataataac tatagaagag ggatataaaa ggaggtcata acgatggcaa
     1261 gagttaaaaa agctatgaac gctcgtaaaa agcataagaa aatattaaaa cttgcaaaag
     1321 gattcagagg atctagaagc aaattatata gaccagcaaa tacattcgta atgaaagcat
     1381 taaaaaatgc ttatatagga agaaagttaa agaagagaga cttcagaaaa ctttggatac
     1441 aaagaataaa tgcagctgct agaatgaatg gaatatctta ttcaagatta atgaatggat
     1501 taaaattatc tggtgttgaa gttaacagaa aaatgttatc tgaaatggct atacaagacc
     1561 cagaaggatt tgcaaaatta gcagaagttg ctaaagcaaa attagcttag tattgaatca
     1621 tgagatgtaa agtctttgag ttatataaat aattcaagga cttttttatt ttttgaaaaa
     1681 tacttattag tgtcatgtac aaccaatttt ataatgataa ataaaataag caatacaata
     1741 gaattttatg agatttatta taagaattgt tacaattatt tatgatagct ataaaaagtc
     1801 atagtaaaaa tgatattgat gtatgttgat atatgtataa agtttccaat caatcttgaa
     1861 ttaatattat caacatgata atattatctt attaataata taaataaggg ggacataata
     1921 tgaatgaaaa actaatggat tgttttgtaa acccaattaa atgcaagtta ttgattgaaa
     1981 ttttttcaaa taatcgaact acagctaaac aattaggaga aaaatttcaa gatatatcaa
     2041 aggcaacact ttatcgttac cttaataaaa tgttgaaaga tgatattata aaagtggttg
     2101 aagaaaataa aataagggga actgttgaaa aagtatatgc tatgaagtta gatatagctg
     2161 atgatgtgaa caaaattata gatgagaact ctggagaaga atatgctcaa ctgtttactc
     2221 aatatatgat gggattttta agagaatttt atgaatacgc tgaaagaaag gaaaaggata
     2281 tcctaaaaga tggttcagga ttttctgtct gtccagtata tgctagtatt gatgagatag
     2341 ttgaagtgag taaaaaaatt gctagtatac tagaacctct aagaaataac tcacatgaag
     2401 aaggtagaag aatgcattct ataggagtga taattactcc acctaaaaag gatgaaggga
     2461 aataaatctg atttacatgg ggtagagcca atttatacag ggaatcgttt tatgatttac
     2521 atatgtatcc agagcaaagt atctctgctt ggattgtact tcattgtacc atttcatcca
     2581 atgacttgga gtggtacaat gaagtttttt aatttttcat attaatgcta aagaaatgaa
     2641 ctttagacca catatgcaat taggttgcat tatacaatta gaccacatat acaaaataaa
     2701 aatcagtatt tttatagcag ataaattgac tgtcattctt cttaaaaatc atctattagt
     2761 tattgttagc atattgaaag aaattatagt aatctattga ataaatgaat attgcctata
     2821 atcatataaa ctgctatagt ttaaaataaa gcaggacgtt aaattttata gtaatctaaa
     2881 aatttaagag ggggatttta tgaaaataaa atttgttatt atgccattgg tgatattttt
     2941 aatggtatta tttgtggggt gtcaaagtac aaaagataca gaagttaaag caaaagatac
     3001 agaaattaaa atattaggga caggtgattt acattcaatt ttgacagact ctatggtttc
     3061 gtatgtaaat gaggaaaggg aaaagaataa aaatctgctt atggtagatg cgggagattt
     3121 ctatggtaca caaagtagag aaatgtggga atggtcttct ggaaaaaagt taattaatat
     3181 taaaagaaat ggtagagcag agtatgatga cataattaag agtagtaaag atgaagtacc
     3241 tatagttaaa gatatggcaa agttgaaata tgatgctgta gttttgggag ataatgaatt
     3301 tgtttcaaat gataagcaaa gtcttgataa actggtttct gattttaaaa ataataatat
     3361 gccattggta tcagcaaata tatatgaaca atctggagaa aattatgtac aaccatatgt
     3421 aatgaaaaat attaagacag atgagggcaa tgtaaaagtt ggaatactag gtcttactat
     3481 aaaagaagta ggggaaaccc ttggtcttga ggattttgaa aaggataaaa aagctagaga
     3541 gcttggagaa caagctgatt acaaaggaaa attatatgca aatgatttag tagaagatgc
     3601 aaaaaaatgg ataaaagtta tggaaaaaga aagtccagac ataatagttg cagttgtaca
     3661 ctcaggggaa gaacctaaaa cagatagaaa tccaggaaat agaataaaag agttagcaac
     3721 tacagtggat ggaatagatg caattgtagc tggtcatact catgaaaaaa tagatgaaca
     3781 caaatataaa aataaatctg gagatgaagt aattgttaca caaccaggtt ggcatggaga
     3841 tagagtatca gagattaatt tcaaactaaa taaagagaat ggaaagtggg acataaagga
     3901 aaagtctagt aaattaaaaa taattgataa agaagtaagt atagttgcta ctgcagattt
     3961 acattcacat ttctctgata aattaacagt tgaccttgtt aatgaacgaa gtaacccaat
     4021 tgaacctgtt gtagtagatg caggcgactt cttagaccct caaacagatg aaatgactca
     4081 atggtataaa gagtggaaaa gtataagaga taataatagt gataaaacga ttagtagatg
     4141 cccaatggtc atggatatga atcagggaat gtatgatgca gtagtacttg gaaatcatga
     4201 atttgtatct gaaaaagatg aattttggtc agatgaacgc cttttagatg ctgttataga
     4261 ggattttgaa caggtggcaa tttctgtgtt atcggcaaat atatataggc aatctggtaa
     4321 aaattatgtt aaaccatata ttataaaaga tgtggagacg agtgaaggta atgtaaaagt
     4381 aggaatattg ggacttacta taaaagaagt aggtaaaggt cttaaaaatg taaacttaca
     4441 agagcaattt caatataagg ataaattgta tgcaaatgat ttagtggaag atgcaaagaa
     4501 atgggtaaaa gttatgaaaa agaaaaaacc agatataata gttgcagttg tacattcagg
     4561 agaagaacca aaaaatccaa aacatccagg aaatagaata aaagaactag caactacagt
     4621 agatggtatt gatgcaattg ttgctagtca tactcatgaa aaaatagatg agcatgaata
     4681 taaaaataag tctggagaaa aagttatagt gacacaacca ggtgagcatg gtaaatatta
     4741 ttctaaaatt acctttaaag taaataaaga aaagggttcg tggactatta atgataaatt
     4801 cagtaaaaca attgaggttg aataacttta taaaatttat agataaagta aattatctga
     4861 tattggcatg atatataaga taaagtttct aatattagta taatgcataa tgtgaaatat
     4921 tttaataata agagaataaa caagacaaat aaaaatattt taacatgtaa gatatagtat
     4981 gaactattat actaatgctt actttataat ttgaacatgt tagaataagt cttatttgaa
     5041 aattttagtg tactagagta agtattattt tactagtaaa aaatattgag aactatatgt
     5101 agatttgctt aaaaaggaat ttacatatag ttcttttcgt ataataaata gtacaatctt
     5161 atatggtaaa gataaagtgt gatattaaat tttattatca tccaaaatct aaggaggagt
     5221 ctcttatgaa aataaaattt attatagtag catcaataat atttttaata gtattacttg
     5281 tggggtgtca aagtactaaa gacacagaaa tcaatgtact cgccacaagt gatttacatt
     5341 caattattcc agataattta atttcctatg tagaagagga gagaaaatat gatgagaatc
     5401 tacttatggt agatgcagga gatttttttg acatgcaaag tggtgagatg aataagtggt
     5461 ttactggtca aaaattagta agatttaaag atgggatacc agagtacaaa aaaatagctg
     5521 aaccacgtga aggagaagtt ccattggcta aaaaaatggc aaagctgaag tatgatgcag
     5581 ttacatttgg gaatcatgaa tttgtatcta atgacaagca gagccttgat aaattggttt
     5641 ctgattttaa aaataacaat ataccattag tatcagcaaa cgtatatgaa caatctggag
     5701 aaaactacgt aaagccatat gtaatgaaaa atataaatac tgacaaggga actgtaaagg
     5761 ttggtatact tggtctgact ataaaagaag taggggaacg ttcaaggttt aaaagttttg
     5821 aacaagataa aaaggctaga gaacttgggg aacaacctca atacaaaggg aaattatatg
     5881 caaatgattt agtagaagat gcaaataagt gggtaaatgt tatggaaaaa aaagaaaagc
     5941 cagatataat agttgccatt gtacattcag gtgaagagcc aaaaaatcct aaaaatccag
     6001 gaaatagaat aaaagaacta gcaacaactg tagagggaat agatgctatt gtagcaggtc
     6061 atacacatga aaaaatagaa cagcatacat ataaaaataa ttctggtgaa gaagtaatag
     6121 taacacagcc tggagctcat ggtaatagta tatcaaagat taattttaag ttggataaga
     6181 agaatgataa gtggtttata aaagacaaat atagtgaatt aaaggttttt ggaaaggaag
     6241 taaatataat tacaacttct ggtctgaatc caaaattttc agatgaaatg gcagttaatt
     6301 tgtttaatga aagagataga gatgttcaac ctatctttat agatactgga gattttttaa
     6361 atactaatac aaaagaaatg actaagtggg ctaaagagtg gcaatacaat gaaaataaag
     6421 gcatctataa aaaaataaat gaatggccaa ttgtatatct tggttatgat gctgtagtac
     6481 ttggtagcca tgaatttaca gaggataaag agacttttga ggaaaaaaaa tctacattgg
     6541 attatattat agaaaacttt gaagaaatga agataccagt attatcagct aatatatatg
     6601 atgaatctgg agaaaattat gtaaaacctt atataattca taatgtagaa actagggaag
     6661 gaaatataaa ggttggagta ttaggtctta ctactactga taaggatgaa gaagacttaa
     6721 gtaactcaag cttaagcaaa gactcaaaga cttcgaaaat tcctgaagaa acaggaaagt
     6781 tatatactaa taatctagta aaagatgcaa gcgaatgggt aaaagttatg caaaaggaaa
     6841 gtcctgatgt gattgttgca gttgtacatt cagataataa tagtactcta aaaaatccaa
     6901 gtagtgaagt caaaaaactt gcaatgtcag tagatggaat agatgctatt gttgtaggtc
     6961 atacgaaaaa taaggtagaa gaacatattt acaaaaataa gtctggggat gaggttatag
     7021 taacacaatc tggagaagat tgttattcaa aaataagtct tgaactaaga aaagaaaatg
     7081 gcaagtggaa tatcataaac aaatatagta aagcaataaa aatgaagtga atttattgat
     7141 atataaacca ttattaacac tgatttttat aatataataa atactg
//
LOCUS       DAELLV010000188         7172 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.188, whole genome shotgun sequence.
ACCESSION   DAELLV010000188 DAELLV010000000
VERSION     DAELLV010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7172
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.188"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..942)
                     /locus_tag="KPD58_002918"
     CDS             complement(<1..942)
                     /locus_tag="KPD58_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBG0266652.1"
                     /translation="MFYFLKVKVGDIMDEVKEFLVLEEEEEVRLDIYLAEQLGDMSRS
                     YIQKIIKDGKVKVNNKVEKARYLVKEEDNIVIEIPEPKVLEVLPQDIPIDIVYEDDDV
                     LIINKPQDMVVHPAPGNYENTLVNGILYHCKDKLSSINGVIRPGIVHRIDKDTSGLLM
                     IAKNNYAHNFLAEQLKEHTITREYEFICYGVVKEDKITVDKPIGRNPKDRLKMAVVKD
                     GKNAITHFEVVERFDKFTHMRARLETGRTHQIRVHALSINHPLLGDYVYGPKETKFKL
                     KGQTLHAKKLGFIHPTTKEYIEFNSELPEYFKEVIKKI"
     gene            complement(911..1357)
                     /gene="lspA"
                     /locus_tag="KPD58_002919"
     CDS             complement(911..1357)
                     /gene="lspA"
                     /locus_tag="KPD58_002919"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBG0266653.1"
                     /translation="MLYILIIILLIGLDQLSKIWVLNNLVDVSTIPIINNVFHLTYVE
                     NRGAAFGLLQNNQWIFIIVALLATVFGLYYLNTRKVHIFGRLGIILIISGALGNLIDR
                     VRLGFVVDYFDFRIIWEYVFNIADVFVVVGTVFLCIYVLFFESKSR"
     gene            complement(1667..2446)
                     /locus_tag="KPD58_002920"
     CDS             complement(1667..2446)
                     /locus_tag="KPD58_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG0266654.1"
                     /translation="MKKIIWGICICLLSIVGVYTYNHNTHNQDSSKEAYSLNDNIKDD
                     YEDVIIKKGNEDEKILALTFDDGPDEDFTPQVLDILKKNDVKATFFVVGEKVEYNKEL
                     LKRQYDEGHEIGNHTFTHINVAKNSYSSVEKEITDTQNIIKSVIGVEPKIFRPPYRAM
                     SKSVCDIIVSKGMNIILWSNLDPRDWSNPGVDAIINTILTKVQNGNIILLHDYNTKRN
                     DKSQTIQALEVVIPKLKERGYKFVTVSELIQHLDKNKQVQK"
     gene            complement(2501..2983)
                     /locus_tag="KPD58_002921"
     CDS             complement(2501..2983)
                     /locus_tag="KPD58_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HBG0266655.1"
                     /translation="MDTKKYKDKLLKERENLTNLVEDMKDNTLFGDTTKHTSEKYSSG
                     ELSSYDNHIGDMGTDLYMQNMQNSLINHEEGRLYQIDLALSKIENGTYGICDLCHNQI
                     DLERLDILPDTSLCNDCAKKENNLLGDAMQNPDVEDSNFYSEDLTNLTDLNKNGILRD
                     "
     gene            complement(3249..4670)
                     /locus_tag="KPD58_002922"
     CDS             complement(3249..4670)
                     /locus_tag="KPD58_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HBG0266656.1"
                     /translation="MVSFLGSIVVLIVGYFVYGTFVEKVFGTNDKNQTPAIACRDGVD
                     YVPMKWKRIFLIQFLNIAGLGPIFGAIQGALFGPSAFLWIVFGTIFAGGVHDFASGYL
                     SMKNKGTSASELVGLYLGNGAKIAMRIFSVILLVLVGVVFVTGPAGLLKTLTGVNAQI
                     WVGVIILYYIMATVLPIDKLIGKIYPLFGAALLIMAVGVAGGLIIKGYNIPNINLQNM
                     NPNGTPLFPYLFITIACGAISGFHATQSPLMARCVEKESEARPVFYGSMVAEGIIALV
                     WAAAAMSYFHGIPQLNVIFNDGGAATVVNTVSVGLMGPIGGALAILGVVACPITSGDT
                     AFRSARLTIADAMNYNQDAVKNRFLIALPLFAVGVALTFIDFNIIWRYFSWANQTLAM
                     IMLWTGSAYLVKANKNHYITTLPALFMTVVTFSYIMQAKEGFRLPVNISNGIGVIVAI
                     ILGLLFFKKAKHIKEQNNHKLAS"
     gene            complement(4816..5586)
                     /locus_tag="KPD58_002923"
     CDS             complement(4816..5586)
                     /locus_tag="KPD58_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0266657.1"
                     /translation="MRAIIVEDEFPARKELRYFIENKSGIEVVSEFTNGIEVLDFIQE
                     NKIDVIFLDINIPHLDGMLLAKTLNQFKSRPKIVFITAYESYAVDAFSLDVFDYILKP
                     YSEERIISMLNKLEKSEMSDIELFNVNSNLYKYKKEAVNQEIEEITHKISLWKGDKLV
                     VIDIDDIYYCEANERQTFIYTEKEKFILKEGISEVENLINDKTFFRTHRSYIVNLTKV
                     KEIIPWFNNTYILKLKNSDYEVTVSRSKVKEFRLLMHI"
     gene            complement(5591..6793)
                     /locus_tag="KPD58_002924"
     CDS             complement(5591..6793)
                     /locus_tag="KPD58_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBG0266658.1"
                     /translation="MKTSILIALIEKTSLIVVLFLLITKLKIFKQIFQKEEYSFNDLV
                     CIALVFTFLAIFGTYSGINYMGSIVNTRIISIVSGGILFGPMVGITAGVFSGIHRYFM
                     DIGGITSVPCLLSSILAGVLSGFLYKRIPKQHRVMYGILVGMISESFTILLIYLISYP
                     HSLAIQIIGGIYLPLIVGQIGIGFVISIVEGIEKDKKDIEARNKAEIKALQRQINPHF
                     LFNSLNTIASFIRFSPDKARELIINLSTYLRYNLEYSDNLIDINKEIEQVKSFVEIEK
                     ARFGELLTVSYDIEDVNIKIPSLIIQPLVENAIIHGILESGRAGVVKISIKKLPYSLE
                     NTVRISIEDNGIGISEEIINNVYQDNMPENKIGLYNVHLRLKLMYGRGLNIRRIDNGT
                     LIVFYVKE"
BASE COUNT         2373 a         1259 c          703 g         2837 t
ORIGIN      
        1 tatcttttta ataacttcct taaaatactc aggtaattct gaattaaatt cgatatattc
       61 cttagtagta ggatgtataa atcccaactt cttagcgtgc aaagtttgtc cttttaattt
      121 aaatttagtt tcttttggac catatacata atcacctaaa agtgggtgat ttatagacaa
      181 agcatgaact cttatttgat gagttctacc agtttcaagc ctagctctca tatgtgtaaa
      241 cttatcaaat ctttctacaa cttcaaagtg agtgattgca ttttttccat cttttactac
      301 agccattttt aatctatcct taggatttct ccctataggt ttatcaacag ttattttatc
      361 ttctttaacc acaccatagc atataaattc atactcccta gttatagtat gttcctttaa
      421 ctgttctgct agaaaattat gtgcataatt gttcttagct atcataagaa gaccagaagt
      481 atctttgtct attctatgaa ctataccagg tctaataact ccatttattg atgacaactt
      541 atctttacaa tgatataaaa tcccgtttac taacgtattt tcatagtttc caggagcagg
      601 atggacaacc atatcctgag gtttatttat tattaagaca tcatcatctt catatacaat
      661 atctattgga atatcttgag gtaaaacttc taatacttta ggttctggta tctctataac
      721 aatattatct tcctctttaa ccagatatct agctttttct actttattat taacctttac
      781 ttttccatct tttataattt tttgtatata acttcttgac atatcaccta attgttctgc
      841 tagataaata tcaagtctaa cttcttcttc ctcttcaagt actaaaaact cttttacttc
      901 atccattata tcacctactt ttactttcaa aaaataaaac atatatacat aaaaacacag
      961 ttcctacaac tacaaataca tcagctatat tgaatacata ttcccatata attctaaagt
     1021 cgaagtaatc tactacaaaa cctaatcgta ctctatcaat tagatttccc aatgcaccag
     1081 atataattaa tataattccc aaccttccaa atatatgtac tttccttgta ttaagatagt
     1141 atagtccaaa tactgttgca agtaatgcaa caattataaa tatccattga ttattttgta
     1201 ataatccaaa tgctgcacct ctattttcga cataagttaa atgaaataca ttatttatta
     1261 ttggtattgt tgatacatcc accaaattat tcaataccca tattttagac agttggtcta
     1321 aacctatgag tagaattatt attaatatat atagcaatga tttttccccc tataaattat
     1381 ttaaaaacca attttatttg aagcacacta ttattattat acaaaaaaaa tgctctaaag
     1441 agcaacttta ttaaaattgt attttcttaa taaagctaga acaaaattta aaatagtact
     1501 acttttttta tattgtagat ttctacatat ttaaaatcat atcattatgt ataaaaaatt
     1561 taaaaatacc atactaatca atattttttg tgtattttat agattaaaga ttaatgatgt
     1621 ctgtaattta aacttacaat caccactaat cttcaaacaa atatttttac ttttgcactt
     1681 gcttattttt atctaaatgt tgtataagtt cagatactgt tacgaattta tatcctctct
     1741 cttttaattt aggaattact acttctaaag cttgtatagt ttgtgattta tcatttcttt
     1801 tagtgttata atcatgtaaa agtattatat taccattttg gacctttgta aggatagtat
     1861 ttataattgc atctactcca gggttagacc aatctctagg gtctaagttt gaccaaagaa
     1921 ttatattcat gcctttagaa actataatat cacagacact tttactcatg gctctatatg
     1981 gaggtctaaa tatctttggt tctactccta ttacactttt tataatattt tgcgtatctg
     2041 ttatttcttt ttcaacacta ctataactat tttttgcaac atttatatgt gtaaatgtat
     2101 gattccctat ttcatgcccc tcatcatatt gtctttttaa taattctttg ttatattcta
     2161 ctttttctcc aactacaaaa aaagttgctt ttacatcatt tttctttaat atatcaagta
     2221 cttgtggtgt aaaatcctca tctggaccat catcaaaagt caatgctaaa attttttcat
     2281 cctcatttcc tttttttatt attacatctt cataatcgtc ttttatattg tcatttaaac
     2341 tatatgcttc ttttgaacta tcttgattat gtgtattatg attatatgta tatacaccta
     2401 ctatagataa taaacatata catatacccc atattatttt cttcatgtca ttacctcttt
     2461 tctaattcat tctagcaata tattttataa tgtcagtttt ttaatctctc aatattccat
     2521 ttttatttaa atcagttaaa tttgttaggt cttctgaata aaagttagaa tcttctacat
     2581 cagggttttg cattgcatca cctaataaat tattttcttt ctttgcacaa tcattacata
     2641 aacttgtatc tggcaatata tccaatcttt ctaaatctat ttgattatga cataaatcac
     2701 aaatcccata tgttccattc tctattttag ataatgctaa gtctatttga tacaatcttc
     2761 cctcttcatg atttattaga ctgttttgca tattttgcat atataaatct gttcccatgt
     2821 ctcctatatg attatcataa ctagataatt ctcctgatga atacttttca cttgtatgtt
     2881 tagttgtatc tccaaataaa gtattatctt tcatatcctc tactaaatta gttaaatttt
     2941 ctctttcttt caataattta tctttatatt ttttagtatc catttttaac acctctaatt
     3001 ttaatattta catcataaaa atagtatgtg gaaaagtaaa ttaaacatac aataaaaata
     3061 agatttgatt cgaaaaatgg aatgtctcaa aatgaaattt ttagttcatt taaccctctt
     3121 ttttatatga aatcaaatat atttttatcc tttcagcaat aaaaaagaga cttatctcta
     3181 ttttgagata gtctcttctt tgaacataat ttaaatacaa ttttttaatt tttatagtta
     3241 ttattttact aacttgccaa tttatgatta ttttgttctt taatatgttt tgctttttta
     3301 aagaataaaa gaccaagaat tattgctaca ataacaccta ttccattaga tatattaact
     3361 ggtagtctaa atccttcttt tgcttgcatt atataactaa atgttactac agtcataaaa
     3421 agtgctggta atgtagttat ataatggttt ttatttgctt tgactaaata tgcagaacca
     3481 gtccacaaca ttatcattgc aagtgtttga tttgcccaag agaaatatct ccaaattata
     3541 ttaaaatcta taaatgtaag agcaacacca actgcgaata atggtaaagc tattaaaaat
     3601 ctatttttga ctgcatcttg attataattc attgcatctg ctattgtcag cctagcactt
     3661 ctaaatgctg tatctccaga agttatagga catgccacaa ctccaagtat agctaaagct
     3721 cctccaatag gccccattaa tccaacagat actgtattta ctactgttgc tgctccacca
     3781 tcattaaata tcacattaag ttgaggtatg ccatggaaat atgacatggc tgctgctgcc
     3841 caaactaaag ctattatacc ttctgcaacc attgacccat aaaatactgg tcttgcctca
     3901 ctctctttct caacacatct cgccataagt ggtgattgag ttgcatgaaa acctgatatt
     3961 gctccacatg ctattgttat aaataagtat gggaataaag gtgttccatt tggattcata
     4021 ttttgtaaat ttatatttgg tatgttatac ccctttatta ttaatcctcc agctacacct
     4081 actgccataa ttaataatgc tgctccaaat aaaggatata ttttaccaat caatttatct
     4141 attggtaaaa cagtagccat aatataataa agtattatca ctccaaccca tatttgtgcg
     4201 tttacacctg tcaatgtctt taataatcct gctggacctg ttacaaatac aactccaacc
     4261 aatactaaaa gaataacact aaatattctc attgctattt ttgcaccatt tcctagataa
     4321 aggcctacca actctgatgc agatgtacct ttgtttttca ttgatagata tcctgaagca
     4381 aaatcgtgta ctccaccagc aaagattgtt ccaaacacaa tccataaaaa tgctgatggt
     4441 ccaaataatg ctccttgtat agctccaaat atcggaccta accctgctat atttagaaac
     4501 tgaattaaaa agattctctt ccatttcatt ggcacataat ctactccatc cctacaagct
     4561 atagctggag tttgattttt gtcattggtt ccaaaaacct tttctacaaa agtaccatag
     4621 acaaaataac ctactataag tactactatt gaacctaaaa agcttaccat aaattcatcc
     4681 ccttagtttt tatataattg ctttataaat ttttatgagt ttaagaaaac tatttccagt
     4741 tcaaatagta atactttttt atgctaatat aaatattaat tacattaagt gcatatttaa
     4801 gtacatgaaa tgactttata tatgcattaa cagcctaaat tcttttactt tgcttctact
     4861 tacagttact tcataatcac tattttttaa ttttaatatg tatgtattat taaaccatgg
     4921 tataatttct tttactttgg ttaagtttac gatataagac ctatgtgttc taaaaaatgt
     4981 tttatcattt ataagatttt cgacttctga tatgccttct tttaatataa acttttcttt
     5041 ctcagtatat ataaatgtct gcctttcatt agcttcacag taatatatat catcaatatc
     5101 tattacaact aatttatcac ctttccaaag gctaatttta tgtgttattt cttctatctc
     5161 ctgatttact gcttccttct tatatttgta taaattacta tttacattaa atagttcaat
     5221 atcactcatc tcactttttt ctaatttatt cagcatagaa ataattctct cttcagaata
     5281 tggtttcaat atataatcaa atacatccaa agaaaaagca tctactgcat aactttcata
     5341 tgctgttata aaaacaattt taggtcttga tttaaactga tttaatgttt ttgcaagtag
     5401 cattccatca agatgaggta tatttatatc taaaaaaatt acatctattt tattttcctg
     5461 aataaagtct aacacctcta taccattggt aaattcactt actacctcga ttccactttt
     5521 attttcaata aaatatctca attcctttct tgctggaaat tcatcttcca ctattatagc
     5581 tctcatactt ctactccttt acataaaata ctatcaaagt cccattatct attcttctta
     5641 tattaagccc tcttccatac atcagtttaa gccttaaatg tacattataa agcccaatct
     5701 tattttctgg catattatct tgatatacat tatttataat ttcctcactt atacctatac
     5761 cattatcctc tattgatatt ctcacagtat tttctaatga atacggtaac ttttttatgg
     5821 atattttcac tacaccagct ctaccacttt ctaaaatacc atgtattatt gcattttcca
     5881 ctaatggttg tataatcaaa cttggaatct ttatatttac atcttctata tcataagata
     5941 cagttagtaa ttctccaaac ctagcttttt ctatctctac aaaagattta acttgttcaa
     6001 tctctttatt tatatcaatt aagttatcac tatattcaag attatatctt agataagttg
     6061 ataaatttat tattaactct ctagccttat ctggactaaa tcttataaat gaagctattg
     6121 tgtttaggga attaaacaaa aaatgtggat taatttgtct ttgaagtgct tttatttctg
     6181 ctttatttct cgcttctata tcttttttat ctttctcaat cccctctact atagatataa
     6241 caaatcctat tccaatctgc ccaactatta aaggtaaata tattccaccg attatttgaa
     6301 ttgctaaaga atgtggatat gatattaagt atataagtaa tatagtaaaa ctttcagaaa
     6361 tcatgcctac caatattcca tacattactc tatgttgttt tggtatcctc ttatatagaa
     6421 atcctgataa tacacctgct agtatacttg ataaaagaca tggaacagat gttatccccc
     6481 caatatccat aaaatatcta tgaatccctg aaaaaactcc tgctgtaata cctaccatcg
     6541 gaccaaataa aatgccacct gatactatag atattattct agtgtttact attgacccca
     6601 tataatttat accactataa gttccaaata tggctaaaaa agtaaaaact aatgctatac
     6661 agaccaagtc attaaaacta tattcttcct tttgaaatat ctgcttaaat atttttaact
     6721 ttgttattag aagaaacaat actacaataa ggcttgtctt ttctattaat gcgattaaaa
     6781 ttgatgtttt cataatcccc ctttaaaaaa taaattttta cacactctaa atttattatg
     6841 gttgttataa attacatatt tgaagtaatc ctatcacaaa actttccaca tttaatcttt
     6901 taattaaata agaaatagtt gtacttctat taacaactaa tactagctag cataagcttg
     6961 taataacttg tagttattat taactttaca attctagtat ttaattttat actttagaaa
     7021 tctcttttct tagaaataat actttcgaat atcatataat acaatattat gtagattctt
     7081 actcatacaa catatttctt taaaaatttt atactattct ttgttcaaat atctcatttg
     7141 atttaattgt gttaaacaca cattatcatc tc
//
LOCUS       DAELLV010000189         7035 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.189, whole genome shotgun sequence.
ACCESSION   DAELLV010000189 DAELLV010000000
VERSION     DAELLV010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7035
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.189"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            107..1000
                     /locus_tag="KPD58_002925"
     CDS             107..1000
                     /locus_tag="KPD58_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG0266659.1"
                     /translation="MQLYNTPFNTNKHLIYFFTFTLVLNWIFSLTPALLGITNTILGE
                     IIFYLAYTSPAIVGLFFISTIYPQSAKQDFIYRYLSFKEIRIKWILLTILFFALTFIT
                     SLIIGNYFNVKTSDIDWIHIAVFRPSKIAYMLLASFILSILSQEAGWRGYAIDKLLVR
                     FGFIGSSIILGLVCGIWYLGSYFTPNQIPYNLAQYSLFDAFLFIPNIVLLNFIINFVY
                     INTSRSILSAGLVHMMYYFFNIQLLLHYPIKLCVIAQYVQIIFGLIFLVYMISSNKFK
                     QKVNSEIEQIKSDDFEFELDW"
     gene            1178..2002
                     /locus_tag="KPD58_002926"
     CDS             1178..2002
                     /locus_tag="KPD58_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0266660.1"
                     /translation="MFKIGEVSKLTQISIRMLRYYDELGILKPAEIDKYTGHRLYSVE
                     QISILQRIVLLRDSKFSVAEIANIVHNWNDEFVIKELNRKKNEIQKEINQEQQRINKI
                     DKFIEAINCDKDEIHYNVVFKKIPSYKIISLREIIPDYQSEGILWEKLSKFIKEEHIE
                     VSRQSNNNIAFYHDEEVKDNGVDIEVGMVVKKIGKNKSGFIYRETEEIDMMACTMVYG
                     PYENIAGAYESFCYWLDKNSDYQISGINRQIGHKGEHNEINPENYLTEIQIPLIKV"
     gene            2101..2709
                     /locus_tag="KPD58_002927"
     CDS             2101..2709
                     /locus_tag="KPD58_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0266661.1"
                     /translation="MGKLLRGKIMKSDSTKEQLINATIQLLSSHRDVSSITAREIVAK
                     ANVNLAMINYYFKSKEELINISIKKFLKRHMDEYKATHDTKNLAPKQQLREILIAFFD
                     FIGEHSQFIKISVPYILIQEEIVYPFEILPLIKAHYENQNNKKTEAEYRTIAYQITSF
                     IQLLFLRSNAFFKYSGIDILNIEQRNGLIDFQLDLLLVDITK"
     gene            2813..2989
                     /locus_tag="KPD58_002928"
     CDS             2813..2989
                     /locus_tag="KPD58_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266662.1"
                     /translation="MFFTFIGVISAILLLCTIICGLWIKLNNRNNDSNSHFTFSILSI
                     AFSLLIVVAFLVRN"
     gene            4097..4849
                     /locus_tag="KPD58_002929"
     CDS             4097..4849
                     /locus_tag="KPD58_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0266663.1"
                     /translation="MLKVNQLCKSYKTGSKTYPVLKNISFEINKGEFVAIMGSSGSGK
                     TTLLNCISCFIPIDNGEIILGNNNIRNLKEKELSEVRNQQLGFVFQDFMLFDGLSVFE
                     NICVPQIIKGSQVLQMENRAINLCELFGIEHIRKKYPAEISGGEKQRTAVARALMNNP
                     DLILADEPTGNLDSKSCRTVIESFLKAKQELDATILMVTHDSFAASFCDRVIVLKDGI
                     IYKEVVKSNSRHKFLDELLEVLKEMGGAQDDN"
     gene            4839..6950
                     /locus_tag="KPD58_002930"
     CDS             4839..6950
                     /locus_tag="KPD58_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0266664.1"
                     /translation="MTINKLYSKLRAYNWKNYTTLFFCIILSVVLVTSYALIYFSPTI
                     QNILPTGGDSRKQATMIFSVAILGCAVFTTYASSLFLKFKSKEIGIFMALGTDKKKLK
                     KLLFSEILLISIVCCFLGLILSIPVSFGIWKIFQLVIIDTREMTYHFGTQGFIYGFIF
                     SVFVTLCIFILSVKFINRTNIIDILNDQRISEPIQDVKDWYGILGIIFIILGIFLGYL
                     VPSLTINKLNYRLPSVWSFTYLLSLIGIYMIMTYIVVHAKRGKHPEKYYKNIISTSMM
                     RFMGRQTVKNMCVISFLIAGALFAVFYVPNMVTGIYDTLKNNPIDYTFTYNQRDKQVS
                     KKDIEQLADKHNIKIQNYMEIPSIELIVDGVESIYHKDGTIEKVYMKKSSYDMFFNSK
                     DFSKLIGKPITIKNGEYLAVVNKGNENQDYKYTKFISSPVTEKEIPFRYAGKIIFNGS
                     FFQNRSMNAYVLNDEDYKSFTKDLSIKNYNNSILFNVDKDTYAFANDLKKEIIKRTSK
                     EMGMFSGYDEYVKKRYESTGRKYFMDEEYPSGEGHLKLDPNNNQLYLNWKYYPNFKIL
                     QSQDMIKNMAVFLMLFIYVAIICFTAVGIIAYTRGVTIAINHKQVFMDLNRLGANNDY
                     IKFCIRNQLKKVFSYPLIAGSFLIYLFNFMIMLNNDGRIVNSEVNALLINLGFIGICG
                     VYMYLIYLLTYKKFKKIIGIL"
BASE COUNT         2632 a          921 c          942 g         2540 t
ORIGIN      
        1 attataggca ataactaaca tcaagcgata acttaataca tataaaaata aataaacaat
       61 taataaaaat taaaacttca ttataaaacg gaaggatagt gaaaaaatgc aactatataa
      121 cacccccttt aatacaaaca aacacttaat ctatttcttt acatttacgc tagtattgaa
      181 ctggatattc agtcttacac ctgcgttgct cggaattaca aatacaatac ttggagaaat
      241 cattttttat cttgcatata catcaccagc tattgtaggt ctatttttta tttctacaat
      301 ctatccacaa agtgcaaaac aagattttat ttatagatac ctaagcttta aagaaattag
      361 aataaaatgg atactattaa caatattatt ttttgcttta acttttatta cttcgttaat
      421 aatcggaaat tactttaatg taaaaacttc tgatatagat tggatacaca tagcagtgtt
      481 tagaccttct aaaatagctt atatgctatt agcaagtttt atattgagta tacttagtca
      541 agaagctgga tggagaggat atgccattga caaattatta gttagatttg gatttatagg
      601 ctcttcaatt attttagggc ttgtttgtgg aatctggtat ctagggtcgt attttacacc
      661 taatcaaata ccatataatt tggcacaata ctccctattt gatgcttttt tatttatacc
      721 aaacattgta cttttaaact ttataattaa ttttgtatat atcaatacaa gtagaagcat
      781 tttatcagct gggttagttc atatgatgta ctattttttc aacattcaat tacttctaca
      841 ctaccctatt aaattatgtg taatagctca atatgtacaa ataatatttg gtttaatatt
      901 tcttgtatat atgatttctt ctaataaatt taaacagaag gtaaattctg aaattgaaca
      961 gataaagtcg gatgattttg aatttgagtt agactggtaa ttttaatatg aattataaat
     1021 taagataatc tatgatttga tacgattact acttaaatat aatttactct ctataattta
     1081 ttgcaacatc tggaaaataa ttgtatttcc acaaacgctt gaagtatata aaaacaaaat
     1141 atatactgat tataagtgaa aaggaggtca tattttcatg tttaaaatag gagaggtttc
     1201 aaaactaaca caaatttcta ttcgtatgtt gcgttattat gatgaacttg gtattttaaa
     1261 acctgcagaa atagacaaat atacaggtca tagattatac tcagttgaac agatttcaat
     1321 actacaaaga attgtattat tgagagattc aaaatttagc gttgcagaaa tagcaaatat
     1381 cgtacataat tggaatgatg aatttgtaat taaagaattg aatagaaaaa agaatgaaat
     1441 acaaaaggaa ataaatcagg aacaacaaag aataaataaa attgataaat ttatagaagc
     1501 tataaactgt gataaagatg aaattcatta caatgttgtc tttaaaaaga ttccaagtta
     1561 taaaatcata tcacttagag agataattcc agactaccaa tctgaaggta tactctggga
     1621 aaagctatct aaatttatca aagaagaaca tatagaagta tcacgtcaat ccaacaataa
     1681 catagctttt taccatgatg aagaagtcaa agataatggt gtagatattg aagttggaat
     1741 ggttgtaaag aagattggaa agaacaaatc tggatttata tatcgtgaga cagaagaaat
     1801 tgatatgatg gcatgtacta tggtttatgg tccatatgaa aatatagctg gtgcatatga
     1861 atcgttctgt tattggttag ataaaaatag tgattatcaa atatcaggta taaatagaca
     1921 aatcggacat aaaggtgaac ataatgaaat aaatcctgaa aattatctta cagaaattca
     1981 aatccccctt ataaaagtct aatagattag tacttaaata cttaagtact aatcacccat
     2041 tgacatccca aaattttcat gatagtatct aaacgtgtgt taaacatgtt taattttttt
     2101 atggggaaac ttttaagggg aaaaattatg aaatcagatt caacaaagga acaattaatt
     2161 aatgccacaa tccagttact atcttcacat agagatgttt caagcattac ggctagagaa
     2221 attgtagcta aagcaaatgt caatttagct atgattaatt attattttaa atctaaagag
     2281 gagttaatta atatttctat taaaaaattt ttaaagcgtc atatggatga atataaggct
     2341 acacatgata ctaaaaattt agcacctaaa cagcaattaa gggaaatact aattgcattt
     2401 tttgatttta taggtgaaca tagtcaattt ataaagattt ctgtacccta tatattgata
     2461 caggaagaaa ttgtttatcc atttgagata ctacctttaa ttaaggctca ctatgaaaat
     2521 cagaataaca aaaaaactga agctgaatac agaactattg cgtatcaaat aacttcattt
     2581 attcagttgc tttttcttcg ttctaatgct tttttcaagt atagtggtat tgatattctt
     2641 aatattgaac agagaaatgg tttaatagat tttcaattag atttattatt ggtagatatt
     2701 acaaaataat actatattaa ttttttatgt acattttttt attttatggt aacgtttgaa
     2761 aatattctca ttgactaaaa atacttgcat taaataaggg ggaggaattt taatgttttt
     2821 tacatttata ggagtaatat cagcaatttt attgttgtgt acaattatat gtggtttatg
     2881 gataaaactt aataatcgta ataatgactc taattctcat tttacttttt caatactttc
     2941 aattgctttt tcactgttaa ttgtggtggc ctttttagta agaaattaat gtctttgtta
     3001 tgctgaggtt tattattata gatttttatt attgcatgat ttttactata ttatagtatt
     3061 tttattatat tacagggtct tatttattat agtgttttgt tgttacagca ttttattata
     3121 ttacagagtc ctatttatta taggattttt attatattgt aaaatcttat ttattatagg
     3181 attttgttat attacaatct cttatttatt gcagtgtttc tattatattg caaactctta
     3241 tttattatgg tgttttgtta ttgcaacatt tctattatat tgtaaagtct tatttattac
     3301 agttctttat tacactacag acttttatta ttacaatctc ttatttatta cagttcttta
     3361 ttatactaca gacttttatt attacagcat ttctgttaca ttgcaaagtc ttatttatta
     3421 cagttcttta ttatactaca aacctttatt attacaacat tttattattt taacttcttt
     3481 actataatat agtaatattt taatatctca aaataatatc aatcactttt cagcatagtc
     3541 taaaatactt ccactttcta aaattatcta tcatacctat actaataata cttttcaacc
     3601 tttttattat aaatttcaat tgtttttaac aacctattta tttccaaaac tccaatatat
     3661 tttctcaatt acattgtgtt aataacccct aaatcctttc aaattattta caatacattc
     3721 cattaaataa tcttactgct ctataattct ttttttcttt ttaaccctac tatacattat
     3781 ttacatcatt tttttcaata caacattcgt tattctataa cctttttact tttactatat
     3841 accttttttc taactacacc ttcaatgttc cacatcatat tactttacca caacaattaa
     3901 tacctaaaaa taaccctcta aatcctccaa taatataatc aatttaacta taataataac
     3961 ctctcacact atccaaacaa tatattttca aacattacaa aaaaacatcc aactattaca
     4021 atatagctca tatccctcct cccctttcaa atgctatcat tgacttacaa gataaatgaa
     4081 agtgaggatg atatatatgc ttaaagtaaa ccaattatgt aaatcatata aaacaggtag
     4141 taaaacatat cctgtattaa aaaatatttc ttttgaaatc aacaaaggag aatttgtcgc
     4201 aatcatgggt tcatcaggaa gtggaaaaac tactctttta aattgtattt catgttttat
     4261 cccaattgat aatggggaaa taattcttgg aaataataat ataagaaatt taaaagaaaa
     4321 ggaactttca gaagttagaa accaacaact aggatttgta ttccaagatt tcatgctatt
     4381 tgatggtctt agtgtctttg aaaatatatg tgttccacaa attatcaaag gtagtcaagt
     4441 cttacaaatg gaaaaccgtg caattaatct atgtgagcta tttggtattg aacatatcag
     4501 aaaaaaatac cctgccgaaa tatctggtgg agaaaaacag cgtacagcag tagcaagagc
     4561 actcatgaac aacccagatt taatattagc agatgaacca actggtaacc ttgatagtaa
     4621 gtcttgtcgt acagtcattg aatcattttt aaaagcaaag caagaattag atgctactat
     4681 actcatggtt acacacgata gctttgcagc aagcttctgt gaccgagtta ttgtgcttaa
     4741 agatggaata atttataaag aagttgtaaa atcaaattca agacataaat tcctagatga
     4801 attactagaa gttttaaaag aaatgggagg cgctcaagat gacaattaat aaattataca
     4861 gtaaacttcg agcctataat tggaagaact atacaacttt attcttttgt ataatacttt
     4921 ctgtggtatt agtaacttca tatgctctaa tttattttag cccaacaata caaaacatac
     4981 ttccaactgg tggagatagc agaaaacagg ctacaatgat attctcagtt gctatattag
     5041 gatgcgctgt ctttactaca tacgcttctt ccctcttttt aaaatttaaa agcaaggaaa
     5101 ttggtatttt catggctcta ggaacagata agaagaaatt aaaaaaactt ctattttcag
     5161 agatattgtt aatttcaata gtatgctgtt ttttaggtct tatattaagt attcctgtat
     5221 cttttggtat atggaaaata tttcaattag tgattattga taccagagaa atgacatatc
     5281 attttggaac tcaaggattc atatatggat ttatatttag tgtatttgtg acattatgca
     5341 tatttatact ttctgtaaaa tttattaatc gtacaaatat aattgatata ttaaatgacc
     5401 agagaatatc tgaaccaata caggatgtaa aggactggta tggtatatta ggtataattt
     5461 ttattatact cggtattttt ctaggatatc tcgtaccttc actaactata aacaaattaa
     5521 attatagact accatctgta tggagcttta cttatctact ctctcttatt ggaatatata
     5581 tgattatgac atacatagtt gtacatgcta aaagaggaaa acatccagag aaatactata
     5641 aaaacatcat atcaactagc atgatgagat ttatgggaag acaaacagta aaaaacatgt
     5701 gtgttatttc atttttgatt gcaggagcat tatttgctgt attctatgtt cctaatatgg
     5761 ttacaggtat ctatgatact cttaaaaata accctataga ttatactttt acctacaatc
     5821 aaagagataa acaggtatca aaaaaagata ttgaacaatt agcagataaa cacaatataa
     5881 aaattcaaaa ctatatggaa attccttcta tagaattaat tgttgatggt gtagaatcaa
     5941 tatatcataa agatggaact atcgaaaagg tatatatgaa aaaaagctcc tatgatatgt
     6001 tctttaattc taaagatttt tccaagctaa ttggcaaacc aataactata aaaaatggtg
     6061 aatatcttgc tgttgtaaat aaaggaaatg aaaatcaaga ttataaatat accaaattta
     6121 tatcaagccc agtcacagaa aaagaaatcc cctttagata tgctggaaaa ataatattta
     6181 atggttcttt ttttcaaaat agaagtatga acgcctatgt ccttaatgat gaggattata
     6241 aatcatttac taaagattta tccataaaaa attacaataa ttctatacta tttaatgtag
     6301 ataaagatac ctatgctttt gccaatgact taaagaaaga aattataaaa agaacatcta
     6361 aggaaatggg catgttcagt ggttatgatg aatacgtaaa aaaacgttat gaatcaactg
     6421 gaagaaaata ttttatggat gaagaatatc caagtggtga aggtcattta aaactagacc
     6481 caaacaacaa tcagttgtat cttaattgga aatactatcc taactttaaa attttacaaa
     6541 gccaagatat gataaaaaac atggcagtct tcttaatgtt attcatatat gtggcaatca
     6601 tatgttttac agcagtagga atcattgcat atacaagggg tgttactata gctataaatc
     6661 acaagcaggt ctttatggat ttaaatcgat taggagcaaa caatgattat ataaagttct
     6721 gtattagaaa tcaattaaag aaagtatttt cataccctct tattgcagga agttttttaa
     6781 tttatctgtt taattttatg attatgttaa ataatgatgg tagaatcgtc aatagtgagg
     6841 tcaatgctct tttgataaac ttaggtttta tagggatttg tggggtttat atgtacctta
     6901 tatatctatt aacttataag aaattcaaaa aaattattgg tattttataa taaaaaaggt
     6961 tattagattt ttaaagtaat aaaatcattg gtgttttata ataaaaaagg ttattagatt
     7021 tttaaagtaa taaaa
//
LOCUS       DAELLV010000190         6941 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.190, whole genome shotgun sequence.
ACCESSION   DAELLV010000190 DAELLV010000000
VERSION     DAELLV010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6941
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.190"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1713
                     /locus_tag="KPD58_002931"
     CDS             <1..1713
                     /locus_tag="KPD58_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp23"
                     /protein_id="HBG0266665.1"
                     /translation="LSILILVLTMFLFNNLTANAQIKEDSIIGIDRYETAGLIADRQT
                     YDTVILVNGDKNLSDGLSSSGLAGAINAPILLTKKSEIPKATLNRLDNKSLNVVKKVY
                     IIGGYNTIENSVEKDIKRKGIQVERINGNNRIETSYNVAKKINEVSSVKEVMLTNGFV
                     GEADAMSIAPVVAKNKGAIILTDGKSVPFKTEGLNVYAIGGKSAINEDLVNETKAIRI
                     GGNDRFETNKKVMEKFYNGATDFYITNGYQLVDALTLSPLAKEKPIVLVADGSNKGIL
                     KGAKSITKVGGIDANIYKQCLDMIKYNNMDMTPNILKHLTSVEGNEVSEILIESDNLG
                     VVVEKTNTNKFEFDYVSVTNEKNCTFSVYKENSSNNIKNGKLVVSAKKKIDKKDSDLQ
                     DMNGDNMINANKDRMVNVIKIGVPDKMYSNFKVDVESGSVELYNIKGGATVDVNDGIA
                     NIVDNNVTNPFNINTNSGISGVTAETISSEIKFRANNGIVNVTATNINGNISLFGSDN
                     KDTFDGIFKLNLKKEPSNLHLKLIGNGINKLPEGWSKDYILGNGQPIIEVKNNGINNI
                     SIGE"
     gene            2097..3083
                     /locus_tag="KPD58_002932"
     CDS             2097..3083
                     /locus_tag="KPD58_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG0266666.1"
                     /translation="MKKITINDIANLAGVSKSTVSRYLNNKDISDSTKEKIKTIIDEY
                     GYEPNAFAQSLRAKKTYFIGIITPCLDSFVKSKIMMAIDEELKKLKYTSLIINTNRKI
                     RSEIDSISKLASLKVDGIILIGTEITKEHKNEIEKLDIPIVVVGQKVDGINSIVNDDY
                     GAGYKMGQYIANKGYKNIVYLGVDESDISVGLNRKNGVLNGLKDKGYDAKVFYTDFDQ
                     EMSIQRSGEMLESENPDIIICATDNIAIATMKEINKRGKSIPQDISVAGFGGYDVLSI
                     ISPKLTTIKFENKNAGKVAANTIVNLIQERKEPLLKEIKFELIEGESTINKN"
     gene            3252..3400
                     /locus_tag="KPD58_002933"
                     /pseudo
     CDS             3252..3400
                     /locus_tag="KPD58_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423822.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
     gene            3419..4900
                     /locus_tag="KPD58_002934"
     CDS             3419..4900
                     /locus_tag="KPD58_002934"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBG0266667.1"
                     /translation="MYKEPKYRTILESTKEELNKLRNISLNDKYKPLFHIHPQHGLLN
                     DPNGLAYYNGKYHVFYQWYPYDATHGMKHWAYVSSDDFVNWNREDVALIPIESYESHG
                     AYSGNAIEVDGKLHMYYTGNIKYSAEDRYAYQNLAIMNKDGKITKYENNPIVSEIPKG
                     YTGHVRDPKVFKRKDKYFMLLGAQTSDKKGVIIVYESKNSIDWNFKGELNVKNIDEDF
                     GYMWECPDYINIDEKDILIFSPQGVEPKGFDYQNIYNVVYAIGNMDLDNLTFEIDTMK
                     ELEKGFDFYAPQTFIKDSQIILFAWAGMGEVLYPTDKNKWAHCLTVPRKLNIKNNKLL
                     QMPVDELIKLRYDETSGQNTIKNNINIIENDENVYELNINIKNIDSNKFGLELFSSQD
                     EGVKLEFNKLGNIVTLDRSNFKKVFGVEYGTNRKEYINIDENTNIKVLADRSILEIFI
                     NEGEVVFTSRIFAKENSNQIRVYSDKIVEYEYTKFKLKQGIEL"
     gene            4926..5897
                     /locus_tag="KPD58_002935"
     CDS             4926..5897
                     /locus_tag="KPD58_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HBG0266668.1"
                     /translation="MKKVISIGEALIDFIPNQNGGKLKNVSEFRRVAGGAPANVSAVV
                     AKLGGKSSFISKLGKDAFGDYIIDVLNEVNVNTDYVLRTSKANTGLAFVSLKEDGNRD
                     FSFYRNPSADMLLEADEVKKEWFNNCHILHFCSVDLIDSPMKLAHKKAIEYALESNSI
                     ISFDPNIRLPLWDSEQSCKKAISEFLPFAHIVKISDEELEFITGENDIEKSLHKLFVG
                     NVELVLYTKGKDGVDAYTKKVKGMCKGVKVNAIDTTGAGDSYIGSFLYTLLYKQITLN
                     DIKEMKQETLNEYLEFSNYYAARSTTIKGAISSYATKEEITEFIKNL"
     gene            complement(6153..6755)
                     /locus_tag="KPD58_002936"
     CDS             complement(6153..6755)
                     /locus_tag="KPD58_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG0266669.1"
                     /translation="MEVCILMGSPKKKGNTAAILKPFIEELENYNSNIEVIWLYDCKI
                     EPCIACKKCQDNLFDFGCYCKDDVQKIFKKILMCDLIVFATPIYSWYCTSPMKALLDR
                     LVYGMNKYYGNEKGPALWAGKSVALITTCGYRPEKGADIWENGMKRYCKHSQLKYIGM
                     LVERDLGKPNFMNKEKEEHSRQFAKQIYYILSNSIELDNC"
BASE COUNT         2946 a          681 c         1146 g         2168 t
ORIGIN      
        1 ctaagtatat taattttagt tttgactatg tttcttttta ataatttaac tgcaaatgca
       61 cagattaaag aagattcaat aataggaatt gatagatatg aaacagcagg acttatagcg
      121 gatagacaaa cttatgatac tgtaatatta gttaatggag ataaaaattt atctgatggg
      181 ctttcatcaa gtggattagc tggagctatt aacgcaccaa tacttttgac taaaaaaagt
      241 gaaataccaa aagctacttt aaatagacta gataataaga gtttaaatgt tgtaaaaaaa
      301 gtatatataa ttggtggtta taatactatt gaaaattcag tagaaaaaga tataaaaaga
      361 aaaggtattc aagtggaaag aataaatggt aacaatagaa ttgaaactag ttataatgta
      421 gctaaaaaaa taaatgaagt aagtagtgta aaagaggtaa tgcttacaaa tggatttgta
      481 ggagaggctg atgctatgag tatagcacct gtagttgcaa aaaataaagg agctataata
      541 cttacagatg ggaaatcagt tccatttaag actgaaggtt taaatgttta cgctataggt
      601 ggcaaatctg cgataaatga ggatttagta aatgaaacta aagcaattag aatcggagga
      661 aatgatagat ttgagaccaa taaaaaggta atggaaaagt tttataatgg agctacagat
      721 ttttatataa caaatggata tcaattagta gatgcactaa cattatctcc tttagccaaa
      781 gaaaaaccaa tcgttttagt tgctgatgga agtaataaag gtattttaaa aggagctaaa
      841 agtataacaa aagttggagg tattgatgcc aatatatata aacagtgttt agatatgatt
      901 aaatataata atatggatat gactccaaat attttaaaac acttaacttc agttgaagga
      961 aatgaagttt ctgaaatact tatagaatca gataatttag gtgtggttgt tgaaaagacg
     1021 aataccaata aatttgagtt tgattacgta agtgtaacta atgaaaaaaa ttgtactttt
     1081 tctgtatata aagaaaattc ttctaataat ataaaaaatg gaaagctagt tgtaagtgct
     1141 aagaaaaaaa tagataagaa agatagtgat ttgcaagata tgaatggcga caatatgata
     1201 aatgcaaata aagataggat ggtaaatgtg ataaaaattg gagttcccga taaaatgtat
     1261 tctaatttta aagtagatgt tgaaagtggt tcagtagaac tttataatat taaaggagga
     1321 gctactgttg atgtcaatga cggaattgct aacattgttg ataacaatgt aacaaatcct
     1381 ttcaatataa atactaatag tgggatttca ggtgtaacag ctgaaactat atcatctgaa
     1441 ataaaattta gagcaaacaa tggaattgta aatgtaacag ctacaaatat aaatggcaat
     1501 ataagtttat ttggaagcga taataaagat acttttgatg gaatatttaa attaaatctg
     1561 aaaaaagaac catcaaattt acacttgaag ttaattggaa atgggataaa taaattgcca
     1621 gaaggttggt ctaaagatta tattttagga aatggacaac caattattga agttaaaaat
     1681 aatggcataa ataatatatc aattggagaa taaaaacata tgaaaataaa gtttatatag
     1741 ttttaaaaat tatagaattt taaaatattt atatatattc tttaaataaa aaattatttt
     1801 ttaagtgata attaagtgtt tatcgataag gctatggttg taaaaatcat agccttattt
     1861 ttatattata aataaattag agtatttatc aattcatagt agataaaatt gaaattaaat
     1921 ataacaacaa aaaatgaaaa ttacaattaa tttaatgtta aagactcaat tatttggatg
     1981 tggaatggat agtacaagta gtatataaaa agtattgact ggacaaataa ctatatttta
     2041 ttttaaatat ataatataat gtaataaaat atatcaaatg tagaaggtga taacctatga
     2101 aaaaaataac aataaatgat attgctaact tggcaggagt atctaaaagt actgtatcaa
     2161 gatatctaaa caataaagat atcagtgatt ctactaaaga gaaaataaag acaataatag
     2221 atgaatatgg atatgagccg aatgcatttg ctcaaagttt gagagctaaa aaaacatatt
     2281 ttattggtat tataacaccg tgtttagatt cttttgtaaa atctaaaatt atgatggcaa
     2341 tagatgaaga actcaaaaaa ttaaaatata catcactaat tattaatact aatagaaaaa
     2401 taaggtcaga aatagatagt atatcaaagt tagctagtct aaaagttgat ggaataatac
     2461 taattggtac cgaaataact aaagaacata agaatgaaat tgaaaagtta gatataccga
     2521 tagttgtagt tggacaaaaa gttgatggta tcaatagcat agtgaatgat gattatggag
     2581 caggctataa aatgggtcaa tatattgcta ataaaggtta caaaaacatt gtatatttag
     2641 gtgttgatga aagtgatata tcagttggtt taaatagaaa aaatggagta cttaatgggt
     2701 taaaagataa agggtatgat gcaaaagtat tttatactga ctttgaccaa gaaatgtcta
     2761 tacaaagaag tggagaaatg ctagaaagtg aaaatccaga tataataata tgtgcaactg
     2821 ataatatagc aatagcaaca atgaaagaaa tcaataagag aggtaaaagt ataccacaag
     2881 atatatctgt agctggattt gggggatatg atgtattgtc tattataagc cctaaactta
     2941 caacaataaa gtttgaaaat aaaaatgcag gtaaagtagc agcaaatacg atagtaaatt
     3001 taatacaaga aagaaaagaa ccactattaa aggagattaa atttgaatta atagaaggtg
     3061 aaagtacaat aaataaaaat taagatacac attacatata ttagtaatgt gtattttttt
     3121 actaaaaaaa ctataattct aaaaattagg aaaacttttt tgaaagaaat attgcatctg
     3181 aaataaattt atgctaatct ttaattaaac cggtttcgaa accggtttaa ttaaaccgat
     3241 agaatagcaa aatgagtgca aaaataactg attgtaacaa agtatcaaag taattattgg
     3301 agtacatagg aataaacaaa atataaaaga tgtagcacat tgtgtaataa gactaagaat
     3361 tgttttagat agcaataata aagcaaatat aaaattataa aagaaaagga ataaaaatat
     3421 gtacaaagag cctaaatata gaaccatatt agaatcgaca aaagaagaat taaataaatt
     3481 aagaaatata tctttaaatg ataaatataa accactattt catatacatc cacaacatgg
     3541 actacttaat gacccaaatg gattagcata ttataatggt aagtaccatg tattttatca
     3601 atggtatcca tatgatgcaa ctcatggaat gaagcattgg gcttatgtaa gttctgatga
     3661 ttttgtaaac tggaatagag aagatgtagc attaatacca atcgaaagtt atgaatctca
     3721 tggagcatat tcaggtaatg cgatagaggt agatggaaaa ttgcatatgt attatacagg
     3781 gaatataaaa tatagtgcag aagatagata tgcatatcaa aacttagcta taatgaacaa
     3841 ggatggtaaa ataactaaat atgaaaataa tccaatagta agtgaaatac caaaaggata
     3901 tacaggacat gtaagagacc caaaagtatt taaaagaaag gataaatact ttatgttact
     3961 tggagcacaa acaagtgata aaaaaggagt tataatagta tatgaatcta aaaactctat
     4021 agattggaat tttaaaggag aattaaatgt aaaaaacata gatgaagatt ttggttacat
     4081 gtgggaatgt ccagattata ttaatataga tgaaaaagat atattaatat tttcaccaca
     4141 aggagtagaa cctaaaggat ttgattatca aaatatatat aatgtagtat atgctattgg
     4201 taatatggat ttagataatt taacatttga aatagataca atgaaagaac tggaaaaagg
     4261 atttgatttc tatgctccac aaacatttat aaaggactca caaataatac tatttgcatg
     4321 ggctggaatg ggagaagttt tataccctac agataaaaac aaatgggcac attgtctaac
     4381 tgttccaaga aaattaaata taaaaaataa taagctattg caaatgccag tagatgaatt
     4441 aattaaatta agatatgatg aaacaagtgg gcaaaatact ataaagaata atattaatat
     4501 tatagaaaat gatgaaaatg tatatgagct taatattaat ataaaaaata ttgacagcaa
     4561 taaatttggg ctggaattat tttcatcaca agatgaaggt gtaaaattag aatttaacaa
     4621 attaggaaac attgtaactt tagacagaag caactttaaa aaagttttcg gtgttgaata
     4681 tggaacaaat agaaaagaat atataaatat tgatgaaaat actaatatca aagtactagc
     4741 ggatagaagt attttagaaa tttttataaa tgaaggagaa gtagtattta ctagtagaat
     4801 atttgctaaa gaaaattcta atcaaataag ggtttatagt gataaaatag tagagtatga
     4861 gtatactaaa tttaaattaa agcaaggtat tgaattataa aaacacatta taaggggtag
     4921 aaaagatgaa aaaagtaatt agtataggag aagctttaat agactttata cctaatcaaa
     4981 atggtggtaa attaaaaaat gtatcagaat ttagaagagt agcaggagga gctccagcaa
     5041 atgttagtgc tgttgtagca aagcttggag gaaaatcgag ttttatatct aagttaggaa
     5101 aagatgcatt tggtgattat ataatagatg tattaaatga agtaaatgta aatactgatt
     5161 atgtattgag aacaagtaaa gcaaatacag gacttgcttt tgtatcatta aaagaagatg
     5221 gtaatcgtga tttctcattt tatagaaatc caagtgcaga tatgctttta gaagctgatg
     5281 aagttaaaaa agagtggttt aataattgtc atatcctaca tttttgttca gtagatttaa
     5341 tagatagtcc aatgaaatta gcacataaaa aagcaataga atacgcactt gaaagtaata
     5401 gtataataag ctttgaccct aatataagat tacctttatg ggatagtgag caatcttgta
     5461 aaaaggcaat atcagaattt ttaccttttg ctcatattgt taagatatct gatgaagaat
     5521 tagaatttat aacaggagaa aatgatatag aaaaatcatt acataaactc tttgtgggga
     5581 atgttgaact agtattatat acaaaaggaa aagatggagt agatgcatac actaaaaaag
     5641 taaaaggtat gtgtaaaggg gttaaagtaa atgcaataga tacaaccgga gcaggagatt
     5701 cttatatagg ttcattttta tacaccttat tgtataaaca aataacattg aatgatataa
     5761 aagaaatgaa acaagaaacg ttaaatgagt atttagaatt ttctaattat tatgcagctc
     5821 gtagtacaac tattaaaggt gcgataagtt catatgcaac aaaggaagaa ataactgaat
     5881 ttataaaaaa tttatagtag gaatgagtaa taggttttga aggaagtaac atgtaaagag
     5941 tttttaagga tgtttactat gaaaataatt aacatataaa aagcaataag gaggctgtct
     6001 caaaatagag ataagcctct ttatcattgt tgaaatgata aaaaatattt taattattgt
     6061 tactttttag aagaatatat atcttatttt aaataagcaa attatgtaag atttatttat
     6121 attatcaata ctttacttgg agtattatgt ctttagcagt tatctaattc aattgaatta
     6181 gataaaatat aataaatttg ttttgcaaat tgacgagaat gttcttcttt ttctttattc
     6241 ataaaattag gtttacctaa atcacgttca acaagcattc ctatatattt taactgtgaa
     6301 tgcttacagt agcgcttcat accattttcc caaatatcag cacccttttc aggtctatat
     6361 ccacatgttg taataagtgc tactgatttt cctgcccata gagcggggcc tttctcatta
     6421 ccatagtatt tattcattcc atacaccaat ctgtcaagta gtgctttcat tggagaagtg
     6481 cagtaccaag aataaattgg agtagcaaaa acaattaaat cacacataag aattttcttg
     6541 aaaattttct gaacatcatc tttacaataa catccaaaat caaataagtt atcctgacac
     6601 tttttacaag caatacatgg ctctatttta caatcatata accatattac ttcaatattt
     6661 gaattataat tttctaattc ctcaataaaa ggttttaaaa tagcagcagt attacctttt
     6721 ttcttaggac ttcccataag aatgcatact tccatattga tacctccaca gtattttaaa
     6781 atagttttta gtgcttaata tagttttagg atactatgag aatacgttaa caacaataca
     6841 ctttgtatat ttgtaataaa attgaaacaa attgttttat caataaaagt tggaatgtat
     6901 ctataattaa aaatatttta ggtatgtttt tacatatatg t
//
LOCUS       DAELLV010000191         6865 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.191, whole genome shotgun sequence.
ACCESSION   DAELLV010000191 DAELLV010000000
VERSION     DAELLV010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6865
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.191"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            49..1479
                     /locus_tag="KPD58_002937"
     CDS             49..1479
                     /locus_tag="KPD58_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0266670.1"
                     /translation="MNLDLEFYQKILEASHDEICVSDDKGIIIYCNKAFEENYGLKKE
                     DILGKNVSFLEDSGYSTKSPIPIVLKTKTKFSLEQDTQTGKKLIITATPIFDENGNLE
                     FTVENCRDITELNNIKNKLEDTKKQVKKYKSEVETLYRTALRIEDTVIMDGIVMRPII
                     NTVNHVSKTDVSVLLLGESGTGKSSLARYIHHNSNRSNGPFITINCATISPQLLESEL
                     FGYTSGAFTGASTKGKVGLVELANGGTLFLDEIGDIPQNLQAKFLQLIQDRTFTPVGS
                     LKNKKVDIRIISATNVDLVSKVKEKKFREDLYYRLNVIEIKLPPLRERRDNLVEIIKY
                     YFNRYSSDFNLNKTISKEAMDAIANYRFPGNIRELQNIIQKILLTCTDNHITIDDLPN
                     ILTKNIHITNNGNKTHISQINKVIISDSKSTNYKNKNFDTLIKEYEKNIILDAYEKFG
                     SSYKVAKHLEISQSKANRLIRKYTNT"
     gene            1679..2263
                     /locus_tag="KPD58_002938"
     CDS             1679..2263
                     /locus_tag="KPD58_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HBG0266671.1"
                     /translation="MLMNTHFIMAKSILDNIDENKTFFISEKNFIYGNIKPDAFSKYK
                     LKKHYLDESFNMIVSKINYLCSLNIDSLSKYFSVSRLSQELGVICHFLCDFFCVAHSE
                     RWEFKHSLNKHVTYERELGAFAKDVDLSKIKCGTINSSFEKFFTKLYSDYKKKTDYMN
                     DLKFSAYTCNSVINYILDSILSNTIKSYHIANCG"
     gene            complement(2367..3146)
                     /locus_tag="KPD58_002939"
     CDS             complement(2367..3146)
                     /locus_tag="KPD58_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG0266672.1"
                     /translation="MIKHIFCDLDGTLYENGTITKEDIVAIEEIEKKGVQFNVATGRI
                     FKQAHNIIKDSLDMNGYYVCENGSFIYDKDYNVIFKRTIDDNLVKKVIDRFESSDAQL
                     YFKYKGDVIVGSDTTAFRHYSSDFIVDPDFEKRSSFDNLIGNIGVCSENLEELSRIEL
                     YLKSEFSEVLEIYFSGTYTLNIVPKSVSKRGSIEHVIKTLNVSPDEVATIGDSPNDIC
                     MLEGFKYSFAMSKAREDVKQSANYVVDSVKSAIDVIMEINS"
     gene            complement(3204..4460)
                     /locus_tag="KPD58_002940"
     CDS             complement(3204..4460)
                     /locus_tag="KPD58_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HBG0266673.1"
                     /translation="MKAEIISVGTEILLGDIVNTNSQFLAKELASLGIEVYHQSTVGD
                     NKQRLLECFDESLKRSDFVITTGGLGPTGDDMTKETAAEYFGQKLELHKPSLEVLESF
                     FVKTGKKMAENNMKQVYFPKDAIVLKNNNGTAPGAILKKDGKFIIVLPGPPREMKAMF
                     NESVKPYLQQFTNEMLVSKTLRLYGIGESNLELEILDIINEQTNPTVALYAKELEVTI
                     RITAKAENEREAFKLIKPVEEKIKSRVGKYVYTEGDISISEGETALEDAVSKLLVEKN
                     LTIAVAESCTGGLVSSSLINYPGISSVFLEGCVTYSNDSKMKRLGVKRETLEEFGAVS
                     EQTAIEMAEGVAKGLKANIGISTTGVAGPGGGTKEKPVGLVYTAIYINGKTIVKKNIF
                     NGDRRKIRLRATRDLLNELRIQLEKL"
     gene            4718..5617
                     /locus_tag="KPD58_002941"
     CDS             4718..5617
                     /locus_tag="KPD58_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBG0266674.1"
                     /translation="MKIAVIMGGISSEREVSLNSGKEIYNNLDKNKYEVVKVIIDDKK
                     DIFTKIPEDIDFAILALHGKFGEDGCIQSILETMDIPYSGCGPLCSGMCMDKNITKKM
                     LRDSNLPTAPWVLVKSVDEIDYDEIDNIGYPVFIKPNSGGSSVATFFIHSKDEVEEAV
                     RKGLEVDEFVMIEKYIPGGEYTSFILNGEVFPTISIKSDSGFFDYEAKYSVEKGAKEE
                     VVYLDEELQKRVNEISETCWKIFNCKAYVRVDMIISEGIPYVLELNTLPGMTQTSLIP
                     RSAAARGIKYSELLDKLIEYSLN"
     gene            5641..>6865
                     /gene="ddlR"
                     /locus_tag="KPD58_002942"
     CDS             5641..>6865
                     /gene="ddlR"
                     /locus_tag="KPD58_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DdlR"
                     /protein_id="HBG0266675.1"
                     /translation="MIFSNLKLNDNEPIYIQLKNYISDMISKGLIPDNSKLPSTRELS
                     QLLQVSRNSVVLTYEELKSEGLIYSISGKGTFVKSKNKSSNTTWSLNWDCLENTYSKK
                     ANELDIIKSEIPWSSDLISFKSISPDGDLFDIEELKKSFLNRISLEGHKLLNYGYAQG
                     YKPLIDYLLEYMTNKGADISNKDILITNGFTEGFDIIMSSFTQEKDYIICENPTHNTA
                     IKIMKSHNLNILGVDINEDGLDFNMLEDNLVKYKNKIKFAYITPSYHNPTGIVMTPEN
                     RYKFYNLMKKYNIAIIEDGFNEELLYNSSHIFPICSLDNMNNGVVYIGSFSKILFPGM
                     RIGWIFADKKVINKLESVKRCRNIHVSFLDQGILFDYLSNGGFEKYIKKIRKFYGDKF
                     NFAYKCIKKYIKTEYI"
BASE COUNT         2582 a          985 c          904 g         2394 t
ORIGIN      
        1 taaacctatt ttaatatatc attaaaaatt taggaggaaa atataaaaat gaatttagat
       61 ttggaatttt atcaaaaaat attagaagct tcccatgatg aaatatgtgt ttctgatgat
      121 aaaggaataa taatatattg taataaagca tttgaagaaa attatggtct aaaaaaagaa
      181 gatattttag gaaaaaatgt ttctttttta gaagactctg gatattcaac aaaaagcccc
      241 ataccaattg tattaaaaac aaagactaaa ttttcactgg aacaagatac tcaaacaggg
      301 aaaaagctta tcataactgc tacccctata tttgatgaaa atggaaactt agaatttact
      361 gttgaaaatt gtagagatat aactgaactt aataatataa aaaataaact tgaagacaca
      421 aaaaagcaag taaaaaaata taaatctgaa gtagaaacac tatacagaac tgcacttaga
      481 attgaggata ctgtaattat ggacggaata gttatgagac caatcataaa cacagtaaat
      541 catgtttcta aaacggatgt aagtgtactt ttattgggtg aatctggaac aggaaaaagc
      601 tcacttgcta gatatataca ccacaacagc aatagatcta atggcccatt tataacaata
      661 aattgtgcaa ctatatctcc acaacttctt gaatctgaac tttttggata tacttcaggt
      721 gcttttacag gtgcaagtac taaaggtaaa gttgggctcg tagaattagc taatggagga
      781 actctttttt tagatgaaat aggagatata ccacaaaatc tacaggcaaa atttttgcaa
      841 ttaatacagg atagaacttt tactccagtt gggagcttaa aaaataaaaa ggtcgatata
      901 agaattatat ctgctacaaa tgttgattta gtttcaaaag taaaagaaaa aaagtttaga
      961 gaagatttat actatagatt aaatgttata gaaataaaat tgcctcctct tagagaaaga
     1021 agggataatc tagtggaaat tataaaatat tattttaata ggtactcaag tgacttcaat
     1081 ctaaataaaa ctatttctaa agaagctatg gatgctattg caaattatag attcccaggc
     1141 aatataagag aattacaaaa tataattcaa aaaatcttgc ttacttgtac agataatcat
     1201 ataacaatag atgacttacc aaacattctt acaaaaaata tacatattac caataatgga
     1261 aataaaacac atatatccca aattaataag gttattatat ctgactcaaa atctactaat
     1321 tataaaaata aaaattttga cactttaatt aaagagtatg aaaaaaatat aattttggat
     1381 gcttatgaaa aatttggtag ttcttataaa gtagccaaac accttgaaat ctcacagtct
     1441 aaagccaata ggttaattag aaagtatact aatacttaat tttaaatttg ataaataaaa
     1501 acttaacact atttacatat tgtaaatttg gccatttact gttattatga atatggttga
     1561 aattatttaa ataaatactg ttgacttagt ttcatatatt taccattaaa cttttatcat
     1621 aaatatcata tcataataat atatttaata agtctaataa actatacgag gtgtaaatat
     1681 gttaatgaat acccatttta ttatggcaaa atcaatactt gataatatag atgaaaataa
     1741 aacattcttt ataagcgaaa aaaattttat atatggcaat ataaagcctg atgctttttc
     1801 taaatataaa ttaaaaaaac attatttaga tgagtcattt aatatgattg tttctaaaat
     1861 aaactatcta tgcagtctta atatagatag tctttctaaa tacttttcag taagtagact
     1921 tagtcaggaa ctaggagtca tatgtcattt tttatgtgat tttttctgtg tagctcacag
     1981 tgaacgttgg gagtttaagc atagcttaaa taaacatgtt acttatgaaa gagaacttgg
     2041 agcatttgca aaagatgttg atttatctaa aattaaatgt ggtactataa atagtagttt
     2101 tgagaagttt tttactaaac tttatagcga ttacaaaaag aaaactgatt atatgaatga
     2161 tttaaaattt tcagcttata cttgtaatag tgtcataaat tatatacttg actctatttt
     2221 atctaataca ataaaatcat atcatatagc aaattgcggt taacttaaga atttttaaac
     2281 ttatataaac ttataattga atataattaa aatgactgct ctatagaata taattttata
     2341 cttctctagg acagtcattt tttatactaa ctatttatct ccattataac atctatggca
     2401 ctttttacac tatctactac ataatttgca ctttgcttta catcttccct tgccttagac
     2461 attgcaaaac tatatttaaa tccttctaac atgcaaatat catttggaga atccccaata
     2521 gtagcaactt catctggact tacatttaac gtttttatta catgctctat acttcctctt
     2581 tttgacacac ttttaggaac tatatttaaa gtatatgttc cagaaaaata tatttctaag
     2641 acttcactaa actcactttt tagataaagt tctattctgg ataactcttc caaattttca
     2701 gaacatacgc ctatatttcc tataagatta tcaaaactac ttcttttttc aaagtctggg
     2761 tcaactataa agtcactaga ataatgtcta aaagcagtgg tatcactacc cacaattaca
     2821 tcacccttat atttaaaata aagttgtgca tcacttgact caaatctatc aatgactttt
     2881 tttactaaat tatcatctat cgttctttta aaaattacat tataatcttt atcatatata
     2941 aatgaaccat tttcacatac atagtatcca ttcatatcta aactatcttt gataatatta
     3001 tgtgcttgtt taaaaattct acctgtagca acattaaact gtactccctt tttttctatt
     3061 tcttcaattg ctactatatc ttcttttgtt atggttccat tttcatatag agttccatct
     3121 aaatcacaaa atatatgttt tatcataaat taaaagcctc caaaaacaat tttatatttt
     3181 actaatactt taatcaaaat aacttaaagc ttttctaatt gtattctcaa ctcattaagc
     3241 aaatctctag tagctcttaa tcttatcttt cttctatccc cattaaatat atttttcttt
     3301 actattgttt ttccatttat atatattgca gtataaacca gtcccacagg cttctctttg
     3361 gttcctccac caggtccagc tactccagtt gtagatatac ctatattagc tttaagtcct
     3421 ttagcaacgc cctcagccat ttctatagct gtttgctcac ttactgctcc aaactcttcc
     3481 aaagtttctc tttttactcc taatcttttc attttagaat cattagaata tgttacacaa
     3541 ccttcaagaa atacagagga tatacctgga tagtttataa gagatgaaga aaccaaacct
     3601 ccagtacaag attcagccac tgctattgtt agattttttt ctacaagtag cttagaaaca
     3661 gcatcttcta aagcagtctc cccttcagat atggaaatat ctccttcagt atatacatat
     3721 ttaccaactc tactttttat tttttcttct actggtttta ttaatttaaa agcttctctt
     3781 tcattttcag cctttgcagt tattcttatt gtaacttcta attcttttgc atataaagca
     3841 actgttggat tagtctgctc attaattata tcgagaattt ctaattctag atttgactca
     3901 cctattccat ataatcttaa agtcttagat actagcattt catttgtgaa ttgttgtaag
     3961 taaggtttga cactctcatt aaacatagct ttcatttctc ttggaggtcc aggcaatact
     4021 attataaatt taccatcttt ttttaaaatt gcccctggag ctgtaccatt attatttttt
     4081 agtactatag catcttttgg aaagtacact tgtttcatat tattttcggc catttttttc
     4141 cctgttttaa caaagaaaga ttccaaaacc tctagtgaag gtttatgtag ttctaatttc
     4201 tgaccaaagt attctgcagc agtttctttt gtcatatcat caccagttgg cccaagacca
     4261 cctgtagtaa ttacaaaatc acttctcttt aagctttcat caaaacattc taaaagtctt
     4321 tgtttgttat ctccaacagt actctgatgg tatacttcta ttcccaatga tgcaagttct
     4381 ttagctaaaa attgagaatt tgtatttact atatctccca ataatatttc tgttcctaca
     4441 cttattattt cagctttcaa aatttatcac tccttgattt atagccatta agactagtct
     4501 taagttcata acaataattt tagcaaaaat aataacataa ataaagttaa aaaatcattt
     4561 aaacatatct ttaaactaga ttattattct aaaaccacac ataatataaa cttcgtatat
     4621 agtggtactt tcatcctcaa ctgttaattc atataattaa gtatgtaaat aaaaagttta
     4681 ctttaaaata ataaaaatta aaataggagt gtataatatg aaaatagcag ttataatggg
     4741 tggaatatca tcagagagag aagtttcttt aaattcagga aaagagatat ataataacct
     4801 tgataaaaat aaatatgaag ttgtaaaagt aataatagat gataaaaaag atatatttac
     4861 aaagatacca gaagatatag attttgcaat acttgcttta catggaaaat ttggtgaaga
     4921 tggatgtata caatcaatac ttgagactat ggatatacca tactctggat gtggacctct
     4981 ttgcagtggg atgtgtatgg ataaaaatat aactaaaaaa atgcttagag attcaaacct
     5041 acctactgct ccatgggttt tagtaaaatc agttgatgaa atagactatg atgaaataga
     5101 taatataggt tatccagtat ttataaaacc aaactcagga ggttcaagtg tagccacatt
     5161 ctttattcat tctaaggatg aagttgaaga agcagttaga aaaggtctgg aagttgatga
     5221 atttgtaatg attgaaaaat atataccagg tggagaatat acttcattca tattaaatgg
     5281 agaagtgttc cctacaatat ctataaaatc tgattcagga ttctttgatt atgaagctaa
     5341 gtactcagtt gaaaaaggtg caaaggaaga agttgtttac ttagatgaag aacttcaaaa
     5401 aagagtaaac gaaatttctg agacttgttg gaaaattttt aattgcaaag catatgtaag
     5461 agttgatatg attataagtg aaggtattcc atatgttctt gaactaaata ctcttcctgg
     5521 aatgactcaa actagtttaa ttcctagaag tgctgctgct agaggtatta agtacagtga
     5581 actattagat aaacttatag aatactcttt aaattagatg aaataatttg aggtggtaat
     5641 ttgatatttt caaatctaaa actaaatgat aatgaaccaa tttatataca acttaaaaat
     5701 tatataagtg atatgatatc aaaaggacta attccagaca atagtaagct cccttctaca
     5761 agggagctta gtcagctttt acaagttagc cgaaattctg tagttttaac ttatgaagaa
     5821 ttaaaatcag aaggattaat ctactctatt tcaggaaaag gtacatttgt taaatcaaag
     5881 aataagtctt caaatactac atggtcatta aactgggatt gtttagagaa tacatactct
     5941 aaaaaagcca atgaattgga cattataaaa agtgaaattc cttggagctc tgacctgatt
     6001 tcctttaaaa gtatttctcc agatggagat ttattcgaca tagaagaatt aaaaaaatcc
     6061 tttttaaata gaatttcact agaaggacat aaattactca actatggata tgcacaaggt
     6121 tataagcccc ttattgacta tttactagaa tatatgacaa ataagggtgc tgatataagt
     6181 aataaagata tactaataac aaatggattc acagaaggct ttgatattat tatgtcatct
     6241 tttacccaag aaaaagacta cataatatgt gaaaatccta ctcataatac tgctataaaa
     6301 attatgaaat ctcataacct caatatctta ggtgtagata taaatgaaga tggtttggac
     6361 ttcaatatgc tagaagacaa tttagttaag tataaaaata aaataaagtt tgcatacatt
     6421 acaccctctt atcacaatcc tacaggaatt gtaatgactc ctgaaaatag gtacaaattc
     6481 tataatctta tgaaaaaata caatatagca attatagaag atggatttaa tgaagaatta
     6541 ctctacaata gttctcatat atttcctatc tgttccctag ataatatgaa taatggggtc
     6601 gtatatattg gtagtttttc aaaaatttta tttcctggaa tgagaattgg atggatattt
     6661 gcagataaaa aagtaatcaa taagctagaa agtgtaaaaa ggtgcagaaa catacatgta
     6721 tcctttttgg accaaggaat tttatttgat tatttaagta atggaggttt tgaaaaatac
     6781 attaaaaaaa tacgtaaatt ctatggggac aaatttaact ttgcatataa gtgtataaaa
     6841 aaatatataa aaacagaata tatct
//
LOCUS       DAELLV010000192         6717 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.192, whole genome shotgun sequence.
ACCESSION   DAELLV010000192 DAELLV010000000
VERSION     DAELLV010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6717
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.192"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            107..1117
                     /locus_tag="KPD58_002943"
     CDS             107..1117
                     /locus_tag="KPD58_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBG0266676.1"
                     /translation="MHEDKNDIFESIDTEDFDNKTEEECLELVRKNGLNLIYIKNQTE
                     EICLEAVRQNCNAIKCVQNQTEKICIEAVKQDWRMLEFVEEPTEEICMLAINQDGTAL
                     KYIENQTEELCLRAVEKNGAALEYVKEQTEEICIEAVRNSEFGLARVKNQTEKICMEA
                     VKHCSYNLKWVENQTEEICMEAVRQNGLDLKFVKNQTETICLRAVRQNGMALEFVKEQ
                     TVGICLKAVRQYGMALKFVKEQTEEICTEAIKQDKRALSFVKGDKEKYKALYDNNEPF
                     AKRYVRNVIEKENRALIKKGEENAKIKIAGKLYDRGMSFEDISDIVEIEISKLKVSLG
                     VF"
     gene            1253..1741
                     /locus_tag="KPD58_002944"
     CDS             1253..1741
                     /locus_tag="KPD58_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG0266677.1"
                     /translation="MKITVLYQTRSGNTERVAKLIEEGAKKVDGVDVKLMRLDNIDLD
                     YLNESKAVIFGTPTYLANTTWEVKKWFDEDSKKVNLAGKLGAVFATCDYICGGPDVAI
                     LTIVGHLMVKGMLVYSGGGSLGKPFIHLGHVHSIEGPELQDEKAIIFGERIATKAKEL
                     FA"
     gene            1917..3845
                     /locus_tag="KPD58_002945"
     CDS             1917..3845
                     /locus_tag="KPD58_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG0266678.1"
                     /translation="MVWHIEYEIFSAIIVIFLMVYFFRSKFIPTLQNKIYCALLIFSF
                     LFIISNILGSFCLNNIDKIPIFITFLLNQIYLLLLPIPAALVSFYVMAIIYQDIRYMK
                     KRLLFLSIPLIICIILSITNPFTHILFGLSKETGYIRGCGYVATFASFFFYAIYSAFL
                     SFKYRKAMHESKIWAIRGFLIVSIVAIILQAIFLQYLLTGTACACSLLLTYLSMQNRG
                     LIIDDLTGVLNRQSFIQELDLNINTEQSGFIITIALDDFKFMNETFGTKNGDIALKEV
                     GKYLIQIHDVNHVYRIGGDIFSIVVNSQLGIEPDDIIRKIEERFKKPWLVEEISFNLA
                     TSIAVVYYPENAETTEDVIVAIDFSIHEAKRSESRRVVYADASISEKIKKKHVIKNCL
                     KEALKNDGFEVYYQPIFSNSRGRFTSAEALLRLEHKELGFIPPDEFIPIAEQTGLINS
                     IGLVVFEKVCRFIASDEFEDLKLDNIAVNLSVVQCMQKNLADDLLYLMNKYNVSPTKF
                     KLEITETVAMGSFNIIKETMERLIDLGVKFALDDFGIAYSGVTNMLSLPFSVIKLDKS
                     LIWSMNEDSRHKLTVETIIALINKLNMKAVAEGVETIKCAEDLIAMECEYLQGYYFSK
                     PVPEDVFKNLLKENTFTS"
     gene            4022..4432
                     /locus_tag="KPD58_002946"
     CDS             4022..4432
                     /locus_tag="KPD58_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBG0266679.1"
                     /translation="MNKKKILVPIDGTERSMHSLEFIKGIFKKDEVEVEIMNVKELVF
                     IDGISLAEEIKNSENLGRRILDKAAEIMGEYDVKVHFTFGYPGDEIIRKAKEDNVDFI
                     VMTKSTKKGLTRMIGSVTASVVKQAKCIVMIVPE"
     gene            4454..4756
                     /locus_tag="KPD58_002947"
     CDS             4454..4756
                     /locus_tag="KPD58_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBC"
                     /protein_id="HBG0266680.1"
                     /translation="MNKKIVAVCESERSLNSLKTAANDLGFDLVYEIQENDNIKNELS
                     IYDIEDASIVLFVTSDSIESIEKIDRFIDREFYEVDPKYIIEDAKSIVSEILIDLN"
     gene            complement(4973..5665)
                     /locus_tag="KPD58_002948"
     CDS             complement(4973..5665)
                     /locus_tag="KPD58_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBG0266681.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCKEGILRAVPYSSPIVEGFKKVDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESEATKLAILNATSKELQKIKYTYLKSIEELDKVSHGLIEECYDADLQFHKEIILAS
                     HNPIFIKYYELIPKTVSSNQYFGFKYRNSLKDMISHHNKIMIAFDSKDSYLGYTSMYN
                     HLEDVIQLFQHD"
     gene            complement(5691..>6717)
                     /locus_tag="KPD58_002949"
     CDS             complement(5691..>6717)
                     /locus_tag="KPD58_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0266682.1"
                     /translation="CLLRLPVGVFADSINKHKIFILSGIFFAGLASLIRVFSPGGNGF
                     LIANILSGISSSTWISYMVHYTNFFSTEHQQKATSTIILVNNLGMLIGFVMSTLFYEK
                     LGMTALCIMSVLSSVIGTILALNIKNHNSKKIPEKISIKKYLSICKDKRLVFFSFMAL
                     IQQGIQMSTTMSFTTQILKNLGSSSTLIGLSSIFYMVCAVASSGFASTKFCTKYGPKF
                     WIPLVFILVSAYCFLVPTIKNINIIFVLQVLPGISTGILLSYLTSESMKNVPKHQKST
                     AMGFFQAIYAIGMSVFPMIVGKISEYTSIQYGYFILAIIAILASILSYTYYKKVDLTL
                     NYDIIQS"
BASE COUNT         2590 a          711 c         1168 g         2248 t
ORIGIN      
        1 tttatgaaaa aaaacaagac tcaaaaattt ttatattctt tgatagaata aaattgagag
       61 taaatttatt gaattttata aaatttataa ggggtgagtc taagatatgc atgaagataa
      121 aaatgatata tttgaatcaa tagatactga ggattttgat aataaaacag aagaagaatg
      181 tttagagcta gtaagaaaaa atggattgaa tttaatatac ataaaaaatc aaacagaaga
      241 gatatgtcta gaggctgtta gacaaaattg taatgcaata aaatgtgttc agaatcagac
      301 agaaaaaata tgtatagaag cagttaaaca agattggaga atgttagaat ttgtggaaga
      361 gccaacagaa gaaatttgca tgttggctat taaccaagat ggaacagcat taaaatatat
      421 agaaaatcag acagaggagt tatgcttaag agccgttgaa aaaaatggtg cagctttgga
      481 gtatgtaaaa gaacaaacag aagaaatatg tatagaagca gttagaaaca gcgaatttgg
      541 attggcaaga gtgaaaaatc aaacagaaaa aatatgtatg gaagcagtta agcattgtag
      601 ctataatcta aaatgggtag aaaatcaaac agaagaaata tgtatggaag cagtgagaca
      661 aaatgggctt gatttgaaat ttgtaaaaaa tcaaacagaa acaatctgtt taagagcagt
      721 aagacaaaat ggaatggcat tagaatttgt aaaggaacag actgttggta tatgcttaaa
      781 ggcagtaaga caatatggaa tggcattaaa atttgtaaag gaacaaacag aagaaatttg
      841 tacagaagct ataaaacaag ataaaagagc acttagtttt gtaaaaggag ataaggaaaa
      901 atataaggct ctatatgata ataatgaacc atttgcaaag agatatgtta gaaatgtaat
      961 agaaaaagaa aacagagcat tgattaaaaa aggagaagaa aatgcaaaaa tcaaaattgc
     1021 aggaaaactt tatgatagag gcatgtcttt tgaagatata tcagatatag ttgagattga
     1081 aataagcaag ttaaaagtga gtttaggtgt tttttaaagt aataaatgta ggtgtttttt
     1141 atgggaaata gttaataaaa aaataatcga ctagctcttt gtaggacagc aaaattagtg
     1201 atataatttg atgtataata aattattttt attagataag ggggaattaa agttgaagat
     1261 tacagtatta taccaaacaa gaagtggaaa cacagaaagg gtcgctaagc tgatagaaga
     1321 gggcgctaaa aaggtggatg gagtagatgt aaagcttatg agacttgata atattgattt
     1381 agattattta aatgaaagta aagcagtaat atttggaaca cctacttatt tagcaaatac
     1441 aacttgggaa gtaaaaaaat ggtttgatga agattcaaaa aaagttaatt tagctggaaa
     1501 attaggagct gtttttgcta catgtgatta tatatgtgga ggtccagatg ttgcgatact
     1561 tactatagta ggacatttga tggttaaagg gatgcttgtg tattctggag gtggttcttt
     1621 aggaaagcca tttatacatt taggacatgt acattcaatt gaaggtccag agttacaaga
     1681 tgagaaggct ataatatttg gtgaaagaat tgctactaag gcaaaagaac tatttgctta
     1741 atttctatat gcatagtaat gattagagta aatttagggt tttaatggag atttgaagta
     1801 aaagttataa aataaaaaat tttccataaa attactcaag atgaattata atataattat
     1861 aaataaatta tattattaaa atatttaaaa tcaaacgtca atagcgaggg aagtttatgg
     1921 tatggcatat agaatatgag attttttctg caattatagt tattttttta atggtatact
     1981 tttttagaag taaatttata cctactttac aaaacaaaat atattgtgca cttttaatat
     2041 tctcattttt atttataatt tcaaatattt taggttcatt ttgtctaaat aatatagaca
     2101 aaattccaat ttttattact tttttattga atcagatata cttattgtta ttgccaatac
     2161 cagcagcact tgtatcattt tatgttatgg ctattattta tcaagatata aggtatatga
     2221 aaaaaagatt attattttta tctataccac ttataatttg tataatctta tctattacaa
     2281 atccttttac gcatattcta tttggattgt caaaagaaac aggttatata cgaggttgtg
     2341 gatatgttgc aacttttgca tcttttttct tttatgcaat atattcagca tttttatctt
     2401 ttaaatatag aaaagcaatg catgaatcaa aaatatgggc tattagaggt tttcttatag
     2461 tttcaattgt ggcaattatc ttacaggcaa tttttttaca gtatcttctt acaggaactg
     2521 catgtgcatg tagtttactt ttgacatatc tttctatgca gaatcgaggt cttattattg
     2581 atgaccttac tggtgtactc aatagacaat cttttattca agagctagat ttgaatatca
     2641 atactgaaca atcaggattt attattacaa ttgcgcttga tgattttaag tttatgaatg
     2701 aaacttttgg aacaaaaaat ggagatattg cactaaaaga agttggaaaa tatttgattc
     2761 agatacatga tgtaaaccat gtatatagaa tagggggaga tatattttct attgttgtca
     2821 atagtcaatt aggtatagaa ccagatgata ttatcagaaa gatagaagaa agatttaaaa
     2881 agccttggct agtagaagag ataagtttta atttagcaac tagtattgct gttgtttatt
     2941 acccagaaaa tgcagaaacg acagaagatg taattgttgc tatagatttc tctatacatg
     3001 aagcaaaacg ctctgagtca aggcgagttg tttatgcaga tgcgtctata agtgaaaaga
     3061 taaagaagaa acatgttatt aaaaattgtt taaaagaagc attaaaaaat gatgggtttg
     3121 aagtgtacta ccaacctata ttttctaata gcaggggcag gtttacttca gcagaggcat
     3181 tacttaggct agaacacaaa gagttaggtt ttatacctcc tgatgaattt attccaattg
     3241 cagaacaaac aggattgatt aactcaattg gattagtagt ctttgagaag gtatgtagat
     3301 ttatagctag tgatgagttt gaagatttaa aattggataa tatagctgtg aatctttctg
     3361 tagtacaatg tatgcaaaaa aacttagctg atgatttact ttatcttatg aataaatata
     3421 atgtatcacc aactaaattt aaacttgaaa ttactgagac tgtggctatg ggctctttta
     3481 atataattaa agaaacaatg gaaagattaa ttgatttagg tgttaagttt gcacttgatg
     3541 attttggaat tgcttattct ggagttacta atatgctttc tttaccattt tcagtaatta
     3601 agttagataa gagtttgatt tggtctatga atgaagatag taggcataaa cttacagttg
     3661 agacgattat agcacttata aataaattga atatgaaggc tgtagcagaa ggtgttgaaa
     3721 ccattaagtg tgctgaagat ttaatagcta tggagtgtga atatttgcaa gggtattatt
     3781 tttctaaacc agttccagaa gatgttttta aaaatttgtt gaaagaaaat acttttacaa
     3841 gttaacaccc tttatttttt taattacaac tttgttcatt gacactgcca gcgatatatt
     3901 ataaaattaa cacaataata aatattaaat ttattataca tattattaat attaattaat
     3961 ggagtaaaga tttattatat attttattta attatcctaa agtgagaaag gaagatgtga
     4021 catgaataaa aagaaaatat tagtgccaat tgatgggaca gaaagaagta tgcattcttt
     4081 ggaatttata aaaggaatat ttaaaaaaga tgaagttgaa gtagaaataa tgaatgtaaa
     4141 agaactagta tttattgatg gcatatccct ggctgaagag ataaaaaatt ctgaaaattt
     4201 aggaagaaga atcttggata aagcagcaga gataatggga gagtatgatg ttaaagtaca
     4261 ttttactttt ggatatccag gagatgagat aattagaaaa gcaaaagaag ataatgtaga
     4321 ttttatagtt atgacaaaat ctactaaaaa aggtcttaca agaatgatag gttcagtaac
     4381 agcaagtgta gttaaacaag caaaatgtat agtgatgatt gtaccggagt agaattagaa
     4441 aggtatttga aatatgaata aaaagatagt agcagtttgt gaaagtgaaa gaagtttaaa
     4501 ttctcttaag acagctgcaa atgatttagg atttgattta gtttatgaaa ttcaagaaaa
     4561 tgataatata aaaaatgaac tttctattta tgatatagaa gatgcatcca tagtgttatt
     4621 tgttacaagt gattctatag aaagcataga gaagattgat agatttatag atagagaatt
     4681 ttatgaagtt gatccaaagt atataataga ggatgcaaaa tctatagtaa gtgaaatttt
     4741 aatagactta aattaatcgt gggtgtcgca ctaagaatag agcgacgcct ttttatatat
     4801 tttacacaag aaataaagag gttgtatcaa aatatagata agagtctttt tcattgttga
     4861 aatgatgaaa atatatttga tttcatacaa aaaagagtac ctcataacta aaaagttcac
     4921 tttgagacac tccattttta tatgttagtt gcatttaagg taaaatagaa gtttaatcat
     4981 gttgaaataa ctgaattaca tcttctaaat gattgtacat tgatgtataa cctaagtaag
     5041 agtctttact atcaaatgca atcattatct tgttatggtg gctaatcata tccttgagag
     5101 aatttctata tttaaaacca aagtattggt tagaagaaac tgttttaggt attaactcat
     5161 aatattttat aaaaattgga ttatgagaag ctaaaattat ctctttgtga aattgcaagt
     5221 cagcatcata acattcttct atcaaaccat gagaaacctt atccaattcc tctattgact
     5281 ttaggtaagt atattttatt ttttgaagtt ctttagatgt agcatttaaa attgctagtt
     5341 tagtagcttc gctttcgata agttgtcttg ctcttaaaga ttctacataa aattcttgta
     5401 tgttatgatt tttatagatt tcaccatcat caaataggtc aactttttta aatccttcaa
     5461 ctataggact tgaataaggc acagctctaa gtattccttc cttacagagt atatttatgg
     5521 catttcttac aggtattcta cttatgttaa ataggtcagc aatctttctc tcacttggta
     5581 atttactatt aattttaaaa gttccattta gaatgtttaa tttaatataa tcaataattt
     5641 tttcagtggc atttaagctt ttcataaaaa ctcctaaaca tataattttt ttatgattgt
     5701 attatatcat agttaagagt taaatctact tttttataat atgtataaga caggatagag
     5761 gccaaaatag ctataatagc caagataaag tatccatatt gtattgaagt atattccgat
     5821 attttaccaa ctatcattgg aaatacagac attcctatag cataaattgc ttgaaaaaaa
     5881 cccattgcag tagatttctg atgttttggt acatttttca tagactcgct agtaaggtaa
     5941 gaaaggagta tacctgtaga tatacctggt agtacttgta gtacaaatat tatattaata
     6001 ttttttattg ttggaactaa aaagcaatag gctgaaacta aaataaaaac taaaggaatc
     6061 caaaattttg gtccatactt agtacaaaat ttagtagaag caaagccaga agaagcaaca
     6121 gcacacacca tgtaaaatat agaagataga cctatgaggg tagaactaga acctaagttt
     6181 ttaagaattt gagtagtaaa tgacattgtt gtagacattt gaataccctg ttgaattaat
     6241 gccataaagg aaaaaaatac aagtctttta tccttgcaga tagataaata ttttttaatt
     6301 gatatttttt caggtatttt tttagaatta tgatttttta tatttaaggc tagaatcgtt
     6361 ccaataactg aagacaatac actcatgata cataaggctg tcattcctaa tttttcataa
     6421 aataaagtac tcataacaaa tccaatcaac attcctagat tgtttaccaa tattatcgta
     6481 cttgtagctt tttgttgatg ttctgtagaa aaaaagttgg tatagtgaac catgtaagaa
     6541 atccaagttg atgatgatat tccagataaa atgttagcta taagaaaacc attgcctcca
     6601 ggagaaaaga ctcttataag agatgctaaa ccagcaaaga aaattccaga cagtataaat
     6661 attttatgtt tatttatgga atcagcaaat actccaacag gaagccttaa taaacat
//
LOCUS       DAELLV010000193         6715 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.193, whole genome shotgun sequence.
ACCESSION   DAELLV010000193 DAELLV010000000
VERSION     DAELLV010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6715
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.193"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(171..314)
                     /gene="scfA"
                     /locus_tag="KPD58_002950"
     CDS             complement(171..314)
                     /gene="scfA"
                     /locus_tag="KPD58_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HBG0266683.1"
                     /translation="MENKHVKTLSQATLKQSAAKGGCGECQTSCQSACKTSCTVANQE
                     CER"
     gene            complement(380..742)
                     /locus_tag="KPD58_002951"
     CDS             complement(380..742)
                     /locus_tag="KPD58_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HBG0266684.1"
                     /translation="MGKSNYIFKGLGYAYIITLAVLLVYNLFLTFTDIGGDNITMVSS
                     FITTISAAIGGFYTSKHMKEKGLMYGLLVGLLYIVCIFLTVFLAQEKFVFEIGMIYKL
                     LLISAAGGIGGVLGVNFK"
     gene            complement(800..1093)
                     /gene="yajC"
                     /locus_tag="KPD58_002952"
     CDS             complement(800..1093)
                     /gene="yajC"
                     /locus_tag="KPD58_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBG0266685.1"
                     /translation="MPQQQIIMSIGLWVVVIAIFYFLMIRPQKKKDKQLKEMRSSLSV
                     GDKVITIGGIVANVAKVEDDVVILELGPNRTKVPFEKWAIGTVKSKKEEIEED"
     gene            complement(1227..2348)
                     /gene="tgt"
                     /locus_tag="KPD58_002953"
     CDS             complement(1227..2348)
                     /gene="tgt"
                     /locus_tag="KPD58_002953"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBG0266686.1"
                     /translation="MYAVRYELIKTCKQSGARLGRLHTPHGIIETPIFMPVGTQATVK
                     SMTPEELKEIGSQIILSNTYHLYMRPGHELIKRAGGLHKFMNWDKPILTDSGGFQVFS
                     LGPLRKIKEEGVEFRSHLDGSKHFLTPEKAMEIQNALGSDIMMAFDECAPYPSDREYV
                     KNSLERTTRWLKRCKDAHNNTDKQALFGIIQGGMYKDLREQSAKEITNIDLPGYAIGG
                     LSVGEPKPLMYDVLEHTTPLMPKDKPRYLMGVGSPDDLVEGVIRGVDMFDCVLPTRIA
                     RNGTAMTSQGKVVVRNATYAEDFTPLDPECDCYACKNYSRAYIRHLIKANEILGARLI
                     TTHNLHFLLNLMKQIRQAIMEDRLLDFRNEFFAKYGYEI"
     gene            complement(2427..2912)
                     /locus_tag="KPD58_002954"
     CDS             complement(2427..2912)
                     /locus_tag="KPD58_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266687.1"
                     /translation="MHSLQKQKKNNPILKIAFIFERVLAVVVLIAVFLGTIDVLRLMW
                     GAYIVDFQNPVQYSQLNDFLAQILLLVIGVELVVMLSLHIPGAFIEALLYAIARKMLL
                     LPKNEGMIDVLIGVIAIAGLFAIKKFLVTKDKSALNIMSIHDEEAIKEQQNKEDEDKS
                     I"
     gene            complement(2924..3949)
                     /gene="queA"
                     /locus_tag="KPD58_002955"
     CDS             complement(2924..3949)
                     /gene="queA"
                     /locus_tag="KPD58_002955"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBG0266688.1"
                     /translation="MKTSDFKFDLPQELIAQVPIEDRASSRLMVLDKETGNIEHKVFR
                     DIIEYLNPGDCLVLNNTRVIPARLIGEKLETGGKIEFLLLKRTEEDTWQALVKPGKRA
                     KVGTKFSFGNGKLIGEVVDLSDEGSRIIKFHYDGIFEEILDELGNIPLPPYITARLDE
                     KERYQTVYSKHNGSAAAPTAGLHFTEELLNKIKEKGVDIAFVTLHVGLGTFRPVKVED
                     VLNHKMHSEYYMVSQEAADKINRAKENGKNVICVGTTSCRTIESACNEYGKMKETSGW
                     TEIFIYPGYKFKVLDKLITNFHLPESTLIMLVSAICGKDNVLNAYNEAVKERYRFFSF
                     GDAMIIK"
     gene            complement(4267..5286)
                     /gene="ruvB"
                     /locus_tag="KPD58_002956"
     CDS             complement(4267..5286)
                     /gene="ruvB"
                     /locus_tag="KPD58_002956"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBG0266689.1"
                     /translation="MQGFEDENRIITSTMKMEDIDIENSLRPKTLEDYLGQEKSKEQL
                     SIFIEAAKSRNEQLDHVLLYGPPGLGKTTLASIIANEMGVNLRITSGPAIERAGDLAA
                     ILTNLNENDVLFIDEIHRINRSVEEVLYPAMEDFCLDIIIGKGPSARSIRLDLPKFTL
                     IGATTRAGMLTNPLRDRFGVICKLDYYTVDELSKIVLRSSSILDAEIQSNGALELAKR
                     SRGTPRIANRLLKRVRDFAQVRADGKITDKVAKDALELLGVDSLGLDFVDEKLLMTII
                     EKFRGGPVGLDTLAASIGEDRNTIEDVYEPYLLQLGFINRGPRGRVAMPLAYEHLKIP
                     YPNEK"
     gene            complement(5393..5995)
                     /gene="ruvA"
                     /locus_tag="KPD58_002957"
     CDS             complement(5393..5995)
                     /gene="ruvA"
                     /locus_tag="KPD58_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBG0266690.1"
                     /translation="MYSYIKGTVEEIYIDSIVVENNGIGYKINVSSNTIMNLQVGEAT
                     KIYTKLIVREDDMSLCGFVSREELKMFELLTSVSKIGPKVALSILSFASPAQLGAYIL
                     SEDIGKLSKAPGVGKKTAERIVLELKDKVDKNNIEFEPTLLSQKPTLISQDESVDALV
                     ALGYTLSESKEAVQKCKKDGMNTEAIIKKALTYIMSKSLK"
     gene            complement(5988..6497)
                     /gene="ruvC"
                     /locus_tag="KPD58_002958"
     CDS             complement(5988..6497)
                     /gene="ruvC"
                     /locus_tag="KPD58_002958"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HBG0266691.1"
                     /translation="MIILGIDPGIAIVGYGIIEYKNSKFKVIDYGAVTTPAHMNISRR
                     LELVYKGIDTIVKNYNIDEVGMEELFFNKNVKTAITVAQARGVTMLACAHNGKPVYEY
                     TPLQVKQGVVGYGRADKAQVQQMVTSFLSLKKVPKPDDVADALAVAICHAHSNKLEKT
                     LKNIGGKYV"
BASE COUNT         2077 a         1284 c          753 g         2601 t
ORIGIN      
        1 cttacatgat tagatatttt tgcataatta aacgataaga aatcaaattt acttctacat
       61 aaatagaata tatattttta tataataatt gaaaataaaa gaggatacct cataatgata
      121 aaagaatcac tttgagatac cctcttacaa acaactaaat caataatttt ctatctttca
      181 cactcttgat ttgcaactgt acaagaagtt ttacaagctg attgacaaga agtttgacac
      241 tctccacatc caccttttgc agcactttgc tttaaagtag cttgagataa agtttttaca
      301 tgtttatttt ccattttcaa cgcctccatt tctaattgtt tcctattgat tatatcataa
      361 aactacttta atataaactc tatttaaagt tgacacccaa tacgcctcca atgcctcctg
      421 ctgctgatat tagcaataac ttataaatca tccctatttc aaatacaaac ttttcttgtg
      481 ctaaaaacac agtcaaaaaa atacatacta tgtacaaaag tcctactaaa agcccataca
      541 taagtccttt ttctttcatg tgttttgaag tgtaaaaccc tcctatagct gcagaaatag
      601 tagttataaa tgatgagacc atagttatat tatctccacc aatatctgta aatgtcaaaa
      661 ataagttata cactaataaa acagccaaag ttattatata tgcatatcct agacctttaa
      721 aaatataatt agattttccc ataaaaaaac ctcctctcaa attaatatat attcgagaaa
      781 aggcttttaa attactaatt tagtcttctt ctatttcttc ttttttagat ttaacagttc
      841 ctatagccca tttttcaaat ggaactttag ttctatttgg tcctaattct aatattacaa
      901 catcatcttc aactttagct acattagcta ctattccacc tattgtaatg accttgtctc
      961 ctacacttag gctgcttctc atttctttta attgtttgtc ttttttcttt tgaggtctaa
     1021 tcattaggaa ataaaagatt gcgattacta ctacccatag acctatactc ataattattt
     1081 gttgctgtgg cattaatttt tcactccttt gtaaattata ctcttttgta aatcataata
     1141 aatgtttata taacaattta ctttctctat ctttgagtac tattgaatat tatatattaa
     1201 aatgattata attaatagtg caaaacctat atttcatatc catattttgc aaaaaattca
     1261 tttctaaaat caagtaatct atcttccatt atcgcttgtc ttatttgttt cattaaattt
     1321 aataaaaagt gaagattatg agtagttatt aatcttgcac ccagtatttc atttgctttt
     1381 ataaggtgtc ttatataagc tcttgaatag tttttacaag cataacaatc acattcaggg
     1441 tcaagtggag taaagtcttc agcataagtt gcatttctaa ctactacttt accttggcta
     1501 gtcattgcag ttccatttct agcgatacga gtaggtagaa cacaatcaaa catgtctact
     1561 cctcttataa caccttctac taagtcatct ggactaccaa ctcccattag gtatcttggt
     1621 ttatcctttg gcataagtgg agttgtatgc tctaacacgt catacataag tggttttggt
     1681 tctccaacac taagtccacc aatagcatat cctggtaaat caatattagt tatttctttc
     1741 gctgattgtt cccttaagtc tttatacatc ccaccttgta ttataccaaa taaagcttgc
     1801 ttgtcggtat tattatgggc atctttacat ctttttaacc atcttgttgt tctttctaaa
     1861 gaatttttta catattctct atctgatggg tatggtgcac actcatcaaa tgccatcatt
     1921 atgtctgaac ccaatgcatt ttgtatttcc atagcttttt ctggagttag aaaatgtttt
     1981 gaaccatcta agtgtgacct aaattctaca ccttcttctt ttatctttct taaaggccct
     2041 agactaaata cctggaaccc tccactatct gtaagtattg gtttatccca attcataaac
     2101 ttatgaagac caccagctct ttttataagt tcatgacctg gtctcatata taaatggtat
     2161 gtgttactaa gtattatttg tgaaccaatc tcttttaatt cttctggtgt catagatttt
     2221 acagttgctt gagttccaac tggcatgaat attggtgttt caataatccc atgtggagtg
     2281 tgtaatcttc ctaaccttgc accactctgc ttacaagtct ttatcaattc gtatcttact
     2341 gcgtacataa agttctcctt aatcatattt atttctcatt atttcttaag atatatttgt
     2401 aataagctat tcatataatt tattgtctaa atagatttat cttcgtcttc tttgttctgt
     2461 tgttccttaa ttgcttcttc atcatgaata ctcattatat taagagcact cttgtctttt
     2521 gttactaaaa acttttttat agcaaatagt cctgctatag ctataactcc tattaaaaca
     2581 tctatcatac cttcattttt aggcagtagc aacatttttc tagcaattgc gtaaagtaat
     2641 gcctcaataa atgctcctgg tatatgaaga gatagcatta ctacaagttc aacacctatt
     2701 actaaaagta atatttgtgc taaaaaatca ttaagctgac tgtattgtac tggattttga
     2761 aaatcaacta tatatgctcc ccacataagt cttagaacat ctatagttcc taaaaatact
     2821 gctattagaa ctactactgc taaaacacgt tcaaatataa aagcaatctt taatataggg
     2881 ttattctttt tttgtttttg taaactatgc atccattacc tccctacttt ataatcatag
     2941 catctccaaa actgaagaat ctatatcttt cttttactgc ctcattatat gcatttaaaa
     3001 cattatcctt tccacaaata gcacttacta acattattag tgtagactct ggtaagtgaa
     3061 aattagtaat aagtttatct aaaactttaa atttataacc tggatatata aatatttctg
     3121 tccatccact agtttctttc atctttccat actcattaca agctgattct atagttctac
     3181 aactggtagt accaacacat attacatttt ttccattttc ctttgctcta tttattttgt
     3241 cagctgcttc ttggctaacc atataatatt ctgaatgcat cttgtgattt aaaacatcct
     3301 ctacttttac tggtctaaaa gttcctaagc caacatgtag agttacaaag gctatatcta
     3361 cacctttttc ttttatttta tttaacaatt cttctgtaaa atgtaatcct gctgtaggtg
     3421 ctgctgctga accattatgc tttgaataaa cagtctggta tctttctttt tcatctagtc
     3481 tagcagtgat atatggaggt agtggtatat ttccaagttc atccaatatt tcttcaaaaa
     3541 ttccatcata atggaatttt ataattcttg aaccttcatc tgataaatct acaacttctc
     3601 caattagctt tccatttcca aaagaaaatt tagtccctac ttttgctctt tttcctggtt
     3661 ttactaaagc ctgccaagta tcttcctctg ttctttttaa taataaaaac tctattttac
     3721 ctccagtttc aagcttctct cctattaatc tagctggtat aactctagta ttatttaata
     3781 ctaagcaatc tcctggattt aaatattcta ttatatctct aaatacttta tgttcaatat
     3841 tacctgtctc tttatctaat accatcaatc ttgagctagc tctatcttct ataggaactt
     3901 gagctataag ttcctgtggt aagtcaaatt taaaatcact tgttttcaat gttaaacttc
     3961 ctctcttaaa atttctccag ctacaataat atcaaaaaaa caacttattt tccacaatat
     4021 taccataatc tatcaagaaa ttgctatttt taacagacac catatccaca atatatagat
     4081 atttttccac gatatacatg tcttatccac aatatataga tacaatacac acaattttta
     4141 tcgtgtttat ccacagtatc tacagatata attaccattt tgtggatatg tttgaatttt
     4201 tagccatttt agaaatattt taatttaatg actcttttat tttataaata ctcgtataat
     4261 ctatttttat ttttcattag gatatggaat ttttaagtgt tcatatgcta atggcatagc
     4321 tactcttcct ctaggacctc tatttataaa tcctaattga agcaaatatg gctcatatac
     4381 atcttctatt gtatttctat cttctccgat agaagctgcc aatgtatcta aacccactgg
     4441 tcctcctcta aatttttcta ttatagtcat aagaagcttc tcatctacaa aatcgagacc
     4501 caaactatcg actcctaaaa gttccagagc atcttttgcc accttatcag ttatttttcc
     4561 atcagctctg acttgtgcaa aatctctaac tctttttagt aatctatttg ctatccttgg
     4621 agtccctctt gaacgttttg caagttctaa tgctccatta gactgtatct ctgcatctaa
     4681 gatgcttgaa gacctaagta ctatttttga aagttcatca actgtataat agtccaattt
     4741 acaaataaca ccaaatctat ctctaagtgg atttgtaagc ataccagctc ttgtagtagc
     4801 tccaataagt gtaaatttag gaaggtcaag tcttatgctt ctagcagatg gccctttccc
     4861 tattattata tctaggcaaa aatcctccat tgctggatat aaaacttctt caacacttct
     4921 attgattcta tgaatctcat ctataaataa aacatcattt tcatttaaat tcgtaagtat
     4981 ggctgccaaa tctccagctc tttctattgc gggtcctgac gtaattctta aatttacccc
     5041 catctcattt gctattatac ttgctagtgt agtttttcca agacctggtg gtccatataa
     5101 aagcacatgg tctaactgct catttctcga tttagctgct tctataaata tactaagttg
     5161 ttctttggat ttttcttgac ccaaataatc ttctaaagtt tttggtctta aactattttc
     5221 tatatctata tcttccatct tcattgtaga agttattatc ctattttcat cttcaaaacc
     5281 ttgcatttat acccctcctc tcatactttt gaaaatctac tttatatatt ttcaaattta
     5341 cataaactcc ttaaatattg ttttaaaatc caaatattat aaaatccaaa tactatttca
     5401 aggatttact cattatatat gtaagtgctt tctttataat agcttctgta ttcataccat
     5461 cttttttaca tttttgaact gcctcttttg attccgacaa agtatagcct aaagctacaa
     5521 gtgcatctac agactcatct tgtgatatca aggtaggttt ttgtgataat aacgttggtt
     5581 caaactctat gttatttttg tctaccttat ccttaagctc taagacgatt ctttctgctg
     5641 tctttttacc aacaccagga gctttggata attttccaat gtcttcactt aaaatatatg
     5701 ctcctaactg tgctggtgat gcaaatgata gtattgaaag cgccacttta ggtcctattt
     5761 tagatacaga tgttagtaat tcaaacatct taagttcttc cctacttaca aaaccacaaa
     5821 gactcatatc atcttctctt acaattaatt tagtatatat ttttgtggct tctcccactt
     5881 gtaaattcat tattgtattg gatgatacat ttattttata tcctatccca ttattttcaa
     5941 caactatact gtctatataa atttcttcta ctgtaccttt tatataacta tacatactta
     6001 ccacctatat tctttagagt tttttcaagt ttgtttgaat gagcatggca aatagccaca
     6061 gctaaagcat ctgcaacatc atctggtttt ggaacttttt ttagacttaa aaatgaagtt
     6121 accatctgtt gaacttgtgc cttatctgct ctcccatatc caactacacc ttgttttact
     6181 tgaagtggag tgtattcata tacaggcttc ccattatgag cacatgcaag catagtaaca
     6241 cctctagctt gtgcaactgt tatagctgtt tttacattct tgttaaagaa taattcttcc
     6301 attccaactt catctatatt gtaattcttt actattgtat caattccttt atacacaagt
     6361 tccaatcttc tcgatatatt catatgggca ggtgttgtaa ctgctccata atcgattact
     6421 ttaaacttgc tatttttgta ttcaattata ccatatccaa ctatggctat acctgggtca
     6481 atccccaata ttatcatttt ttaaaattcc ccctaaaaat aaaagaaata ccaaaatgta
     6541 tttctcataa aaacttatgt tctaaatcat tatatcataa ataatattaa caaatcataa
     6601 aatgctctat gtaaaatata aaatcaaata aaaaagttat atcaaagcaa gattttacct
     6661 caatataact tttatattta ttacaaataa attattgtat ttttatagga attaa
//
LOCUS       DAELLV010000194         6698 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.194, whole genome shotgun sequence.
ACCESSION   DAELLV010000194 DAELLV010000000
VERSION     DAELLV010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6698
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.194"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            73..828
                     /locus_tag="KPD58_002959"
     CDS             73..828
                     /locus_tag="KPD58_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0266692.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KSTLLNIISGLIKESSGQVLLNKHNLSNLAEHRRTQIVSRVFQDPSLGTCPSMTVREN
                     LSLALNKGKLLNLRKCLRYKRDFLENLLEGVSLDLKKYLDVQVKFLSGGQRQSLSLIM
                     SCLTNPSVLLLDEHTAALDPKTSNEVIELTNKIVREKNITTLMVTHNLKHALHYGDRL
                     IMLHKGEVVLDVKGKEKEQLTVEEILEKFEYAV"
     gene            959..1981
                     /locus_tag="KPD58_002960"
     CDS             959..1981
                     /locus_tag="KPD58_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0266693.1"
                     /translation="MINKKRLASLILAGALSISMLTGCSQGGDSGNSKQESNSKGKEV
                     KKIGITQLVEHPALDATRTGFVKALEKNGFKDGENIDIDFQNAQNDMPTTQSIASKFA
                     SDKKDLIFAISTPSAQAAFNATKDIPILITAVSDPVAAGLVKTLEKPGTNVSGTSDFV
                     SVDKGLELLKIFAPKAKTIGVMYNTSEVNSKVQVDALKEYASKNGFKVVEKGITTSNE
                     VNQGISSLVGKIDVLYVPTDNLVASSMPIVSKIATENKIPVIAAESGPVEKGALACQG
                     INYEKLGYKTGEMAVKILNGESVSDMPVATSDDTDIIVNEDILKALGMEKPSNENISY
                     VKTKQE"
     gene            complement(2145..2702)
                     /locus_tag="KPD58_002961"
     CDS             complement(2145..2702)
                     /locus_tag="KPD58_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBG0266694.1"
                     /translation="MTEKEKMLSGKGYYANDELLVKEREYCKKLTRLFNNTLEDEYEK
                     REDILRQLFGSVGKQINVEQNIRCDYGYNIHVGENFFANYDCIFLDVCKIEIGDNVML
                     APNVQIYTAYHPIDAQLRNSGIEYGSPIKIGDNVWIGGGVIITPGITIGDNVVIGAGS
                     VVTKDIPPNTVAVGNPCRVIKKIEE"
     gene            complement(2708..3784)
                     /locus_tag="KPD58_002962"
     CDS             complement(2708..3784)
                     /locus_tag="KPD58_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HBG0266695.1"
                     /translation="MSLYVDIEKDLSSFKLKVEIKQEKGTLGFLGESGSGKSMTLKCI
                     AGLEKPTRGKIVLNDRVLFDSEKKINLSTQDRKVGFLFQNYALFPHMTVSQNIELGLL
                     KLSKSEKKEIVARYLDILKLNGFEGRYPWQLSGGQQQRVALARALATSPDILLLDEPF
                     SALDHHLRSNMEKELMNMLKDYKGDILFVTHDIEEAYRVCDDIIVYNKGEGLPKRPKK
                     ELFESPKYLIEAKITGCKNISKLNRLDKNTIYATDWGCELTLNREIGDNIEYVGIREH
                     HIKVLDSNEDLNEKLCFELINIVENPFTYTIYVRKTDLSNECVPIQIELEKSKMRFKK
                     GDRIYLDFPQEYLFCFRYNYNKKE"
     gene            complement(3785..4468)
                     /gene="modB"
                     /locus_tag="KPD58_002963"
     CDS             complement(3785..4468)
                     /gene="modB"
                     /locus_tag="KPD58_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HBG0266696.1"
                     /translation="MGTDWSPLWISLKTSILSTIITFVIGIFVSYIMANYRGKWKGLI
                     DGLFTLPLILPPTVVGFFLLLLCGKNGFIGKFLLNFDRTLIFSWTATVISAVVVSFPM
                     MYRTSRSAFEQIDNNMLSAARTLGLNEWKVFFKIAVPLAWSGIIGGLVLSFARALGEF
                     GATLMIAGNIPGKTQTMPIAIFFAVEGGDMNKAMMWVMIIVAISIAMILLLNYWSEFQ
                     QKIIGKRCG"
     gene            complement(4617..5429)
                     /gene="modA"
                     /locus_tag="KPD58_002964"
     CDS             complement(4617..5429)
                     /gene="modA"
                     /locus_tag="KPD58_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG0266697.1"
                     /translation="MKKILGILGLVACLTLGTVGCNSNEDKKDDGKQEKTTKSSDSVE
                     LNISAAASLKEAMAKIEEEYKKVDSNVKLTVNYGASGSLQQQIEQGAPCDLFISAGQK
                     QMKALDEEKLLVSDTMKDLVKNDLVLISSADSSVSGMKDLTTDKVKKIAVGEAESVPA
                     GKYADEVLTNLNLKDKLKDKLVFAKDVKEVLAWVQSGNADVGFVYFSDTVNNDKIKVV
                     EKTDEKTHSPITYPVSVIKASKNVDAAKKFEEFLLSESGQKIFEEFGYKKVE"
     gene            5622..6551
                     /locus_tag="KPD58_002965"
     CDS             5622..6551
                     /locus_tag="KPD58_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0266698.1"
                     /translation="MDSLSSLTPVEVAELLKITKNTVYELIKRGELPSYKVGKKIRVD
                     TKDVEEYINSQKTGKVKDTHFNNKNIDINNNFKIIISGQDVILDILARSVEKKLDGIS
                     TFRSYIGSYNALYELYNGRVSIASSHLWDFETDEYNSTFVKKLLPGIPCVLINLAYRM
                     QGFYVAKGNPKEITTWEDLTKPSITIVNREKGSGTRVLLDGKLRLLNFNGKHIKGYDN
                     EELSHLGVASTVSRGIADVGLGNEKAALQVNNIDFIPLHKERYDLVIKKEDLQNPVYQ
                     TIINIINSPEFKAELQGLGGYDLTDTGKTIAKV"
BASE COUNT         2331 a         1093 c          877 g         2397 t
ORIGIN      
        1 taaatatttc actaaaaaaa aacaaatgca aagctagtac caaaaataaa tcagaaaaaa
       61 gaggtgttgt agatgttaca aattaaaaat ctttcaaaga gtttccctaa tccatatggt
      121 gaacctaata ctatctttga aaatttatca atcgatatag aagatggtga atttgtaagt
      181 atcataggta gtaatggaac tggtaaatct actttattaa atataatatc cgggcttata
      241 aaagaatctt ctggtcaagt attattaaat aaacataact tgtctaattt agctgaacac
      301 agaagaactc aaatagtaag tagagttttt caagacccaa gtcttggaac ttgcccttct
      361 atgactgtta gagaaaactt gtctttagct cttaacaaag gaaaactatt aaatctaaga
      421 aaatgtcttc gttacaaaag agatttctta gaaaatttat tggaaggtgt atctcttgat
      481 ttaaaaaaat accttgatgt tcaagtaaaa tttttatcag gaggtcaaag acaatcatta
      541 tcactaatta tgtcgtgctt aacaaatcca tcagttttat tacttgatga acatacagca
      601 gcacttgacc caaaaacatc aaatgaagta attgaattga ctaataagat tgtaagggaa
      661 aaaaatataa ctacacttat ggttactcac aacttaaaac acgctcttca ttatggtgat
      721 agattgataa tgcttcataa gggtgaagtt gtattagatg taaaaggtaa agaaaaagaa
      781 caattaacag ttgaagaaat cttagaaaaa tttgaatatg cagtttaaaa taatatatct
      841 attgtattaa actcggcttt aaaataaagt taaaattaca ataattatgg ttactttgta
      901 ataattcata tgaatatatg aacatatata acaataaatt tttgaggggg atataaaaat
      961 gattaataaa aaaagattgg caagtttgat tttagctggt gctcttagta tatctatgct
     1021 tacagggtgt tcacaaggag gagactctgg taattcaaaa caagaaagta attctaaagg
     1081 taaagaagtt aaaaaaattg gtataactca actggttgaa catccagcat tagatgccac
     1141 taggacaggt tttgtaaaag cattagaaaa gaacggattt aaagatggag aaaatattga
     1201 tatagacttc caaaatgctc aaaatgacat gcctactaca caaagtattg cgagtaaatt
     1261 tgcatctgat aagaaggact taatatttgc tatatctact ccatcagctc aggcagcatt
     1321 taatgctact aaagatatac ctatacttat tactgctgtg agtgaccctg ttgctgctgg
     1381 attagttaaa actcttgaaa agccaggcac aaatgtatct ggaacatcag attttgtttc
     1441 tgtagacaaa ggtttagaac ttcttaaaat atttgctcct aaggcaaaaa ctataggagt
     1501 tatgtacaat actagtgaag taaattccaa agttcaagtt gatgctttaa aagaatatgc
     1561 atctaaaaat ggatttaaag ttgttgaaaa aggtataact acttcaaatg aagttaacca
     1621 aggtatttct agtttagttg gcaaaataga cgtattatat gtccctacag ataacttagt
     1681 ggcttcttct atgcctatag tctctaaaat agctactgag aataaaatac ctgttatagc
     1741 agctgaatct ggtccagttg aaaaaggtgc tcttgcttgt caaggtatca actacgaaaa
     1801 actaggttac aaaacaggtg aaatggcagt taaaatttta aatggcgaat cagtatctga
     1861 tatgcctgtg gctacatcag atgatacaga tatcattgtt aatgaagata tattaaaagc
     1921 tttaggtatg gaaaaacctt ctaatgaaaa tatttcttat gtaaagacta aacaagaata
     1981 gaaagatatt ttctatatac gattaatatg cctgtttcta taaattaatt tatagaaaca
     2041 ggcatatatt tttattttaa tgtattatct atttaaagat tatatgtaaa tgctcttaat
     2101 ttgtttgtat aaattttact tgtataaact ttatacaatc atatttattc ttctattttc
     2161 tttattactc tacatggatt tccaacagct acagtatttg gtggtatatc ttttgtgact
     2221 acacttcctg cacctattac tacattatca cctatagtaa ttcctggtgt tatgattact
     2281 cccccaccta tccatacatt atcacctatt tttataggag aaccatactc tatgcctgaa
     2341 tttctaagtt gtgcatcaat tggatgatag gctgtataga tttgaacgtt tggagctaac
     2401 attacattgt cacctatttc aattttacat acatctaaaa aaatacaatc ataatttgca
     2461 aagaaatttt cgcctacatg tatgttatat ccataatcac acctaatatt ttgttctaca
     2521 tttatttgct tgccaactga accaaatagt tgtcttaaaa tatcttctct tttttcatat
     2581 tcatcctcta aagtattatt aaacaatcta gttaattttt tacaatactc tctttctttt
     2641 actaacaatt catcatttgc atagtatcct ttcccagaca acattttttc tttttcagtc
     2701 atatagttta ctcctttttg ttataattgt atctaaagca aaataaatat tcctgtggaa
     2761 aatctaaata tattctgtca cctttcttaa atctcatttt acttttttcc aattctattt
     2821 gaataggtac acattcgtta gataaatcag tttttctaac atatatagta tatgtaaaag
     2881 gattttctac aatgttaatc aattcgaaac ataacttttc atttaaatct tcattagaat
     2941 ccaagacctt aatatgatgc tccctgattc ccacatattc tatattatca cctatttctc
     3001 tatttaatgt caactcgcat ccccagtctg tagcataaat agtattttta tccagtctat
     3061 ttaatttgga tatattttta caccctgtta tcttagcttc tataaggtac tttggagatt
     3121 caaagagctc ttttttaggt ctctttggaa gaccttctcc tttgttgtac actataatat
     3181 catcacaaac tcggtaagct tcttcaatat catgagtaac aaatagtata tctcccttgt
     3241 aatcttttaa catattcata agttcttttt ccatattgct tcttaaatga tggtccaatg
     3301 ctgagaaagg ttcatctaaa agtaatatat ctggagatgt tgccaatgct ctagctagtg
     3361 ctaccctttg ttgttgacca ccagataatt gccaagggta tctcccctca aacccattta
     3421 atttaagtat atctaagtac cttgctacta tttccttttt ttcactctta cttagtttta
     3481 aaagcccaag ttctatattt tgacttacag tcatatgtgg aaacaaagca tagttttgaa
     3541 ataaaaatcc tacctttcta tcttgtgttg ataaatttat cttcttttca gaatcaaata
     3601 gaactctatc atttaataca attttacctc ttgttggttt ctcaagccct gctatacatt
     3661 ttaatgtcat actttttcca gaaccagatt ctcccaaaaa ccccagagta cctttttctt
     3721 gtttaatttc tacctttagt ttaaaagagg ataaatcttt ttcaatatcc acgtataagc
     3781 tcaactatcc acacctcttt cctataattt tctgctgaaa ttccgaccaa taatttagta
     3841 ataaaatcat tgctatagat attgctacaa taatcataac ccacatcata gctttattca
     3901 tatctccgcc ttcaacagca aaaaatatag ctattggcat agtttgagtt tttccaggta
     3961 tatttccagc tatcataagt gtagcaccaa attctccaag agctctggca aacgacagca
     4021 ccaatccacc aattattcca gaccatgcta atggaactgc aatcttaaaa aatactttcc
     4081 attcattaag acctaatgtt ctagcagctg acaacatatt attatcaatc tgttcaaaag
     4141 ctgaccttga agttctatac atcataggaa aagacaccac tactgctgaa attacagttg
     4201 ctgtccaact aaatattaaa gtcctatcga aatttaataa aaactttcct ataaatccat
     4261 tcttcccaca tagtaataat aaaaagaaac ctacaactgt tggtggcaaa attagtggaa
     4321 gtgtaaatag tccatctata agacccttcc acttacctct atagttagcc ataatatatg
     4381 acacaaatat tcctattaca aaagttatta ttgttgacaa aatagatgtc ttaagtgata
     4441 tccataaggg tgaccaatct gttcccatct ttccctcctt aattttaaat ggaaatagtg
     4501 aaactaataa ctctattaat ttcactattt ccattttatt ataatacaca attattttca
     4561 attttcattt atttatatat aatttatact taattacaca taatttattt ctaagtttat
     4621 tctacttttt tatatccaaa ttcttcaaaa atcttttgtc ctgactcact taataaaaat
     4681 tcttcaaatt tcttagcagc atctacgttt ttactagctt taattactga aactggatat
     4741 gtaataggcg aatgagtttt ttcgtctgtt ttttcaacta cttttatctt atcattattg
     4801 acagtatcac tgaagtaaac gaatccaaca tcagcatttc ctgattgcac ccatgctaaa
     4861 acttctttta catcttttgc aaatactagc ttatctttta acttatcttt taaatttaaa
     4921 ttagttaaca cctcatctgc atacttacca gctggaacac tttcagcttc accaacagca
     4981 attttcttca ctttatctgt agtcaaatct ttcatacctg atacactact gtcagcagaa
     5041 gaaataagaa ctaaatcatt ttttactaaa tctttcatag tatcagaaac taataacttc
     5101 tcttcatcca aagctttcat ttgtttttga cctgctgaaa taaataaatc acaaggtgct
     5161 ccttgttcta tttgttgttg taatgagcct gatgctccat agttaactgt aagttttaca
     5221 tttgaatcaa cttttttata ttcttcttca atctttgcca ttgcttcttt taaacttgca
     5281 gcagccgata tatttaactc tactgaatca ctacttttag ttgttttttc ttgttttcca
     5341 tcatcttttt tgtcttcatt actattgcac ccaactgtcc ccaaagttaa acatgcaact
     5401 aatcctaata ttcccaatat ttttttcata acaatctccc tttttgtaaa tttgatacac
     5461 attttaacat gttttattat gttttatcat gttttgttat atatagttta attatatata
     5521 catgataata aattttcaag taatttattt acactacttt taaatatttt gctacatttt
     5581 ccttttttta atgttaaaat aaccttatat ggaggtggat tatggactca ttatcatctt
     5641 tgacacctgt cgaggtagct gaattattaa agataacaaa aaatactgta tatgaattaa
     5701 tcaaaagagg tgaacttcct tcttataaag tagggaaaaa aataagagtt gatactaaag
     5761 atgttgaaga atatatcaac agccaaaaaa ctggtaaagt aaaggataca catttcaaca
     5821 ataaaaatat agatataaat aataatttca aaattattat ttctggtcaa gatgtaatat
     5881 tagacatttt agctaggtct gttgaaaaaa aattggatgg aataagtaca tttcgctctt
     5941 atataggtag ttataatgct ttgtatgagt tatataatgg cagagtttct atagcatcat
     6001 cccatctatg ggatttcgaa acggatgaat ataatagcac ttttgttaaa aagctattgc
     6061 caggaatacc atgtgtactt ataaatctag cttatagaat gcaaggtttc tatgttgcta
     6121 aaggaaatcc taaagagata acaacctggg aggatttaac aaaaccaagt ataaccattg
     6181 taaataggga aaaaggttct ggaactaggg ttttactaga tggtaaactt agacttctaa
     6241 actttaatgg taaacatata aaaggttatg ataatgaaga attatcacat cttggagttg
     6301 caagtactgt ttctcgtgga atagcagatg ttggtttggg aaatgaaaaa gctgctttac
     6361 aagtaaataa tatcgacttt attcctcttc acaaagagcg ctatgattta gttattaaaa
     6421 aagaagactt acaaaatcca gtttaccaaa ctataattaa cataataaac tctcctgagt
     6481 ttaaagctga acttcaaggt cttggcggat atgatttaac tgatactgga aaaactatag
     6541 caaaagttta agattaaaat cgcaatctat tttataagat ttataatttt tcacaaaatt
     6601 tataacttaa acacgaaata aattgcgatt ttagtgtctc aaaaaattta tgtctttcat
     6661 tttatcatcc aatacaaaat ataatataat taattgtg
//
LOCUS       DAELLV010000195         6582 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.195, whole genome shotgun sequence.
ACCESSION   DAELLV010000195 DAELLV010000000
VERSION     DAELLV010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6582
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.195"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            202..1902
                     /locus_tag="KPD58_002966"
     CDS             202..1902
                     /locus_tag="KPD58_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0266699.1"
                     /translation="MIKQFVKYYKPYKKIFTLDLIAAFLFSLCDLVYPMITRNIMDDV
                     VPNKNLRMLVVFAVALILIFIAKAGLNYFMQYWGHVIGVDMQADMRNEVFTHLQRLPN
                     TYFDNNKSGVTMSRIVNDLMDITELAHHGPEDLFISIVMLVGSFFILIDINIPLTLII
                     FAILPFIIWFAIAKKDKMNIAFMKSRVTIGDVNATLENSIAGMKVTKSFCTEKEELNK
                     FVRSNKLFRRARQDSYKVMAEYYSGMNLYMDILEWVVVIAGGYFTYIGKITLGDFAAY
                     ILYVKMFIQPMKKLINFTEQYQNGMTGFKRFIEIMEQDHQKEAKNPIELENVKGDIEI
                     ENISFTYEDKTQVLDNLSLSIKAGKTIALVGPSGGGKTTLCNLLPRFYEFDKGDIKID
                     GKSIKDVSLKSLRKNIGIVQQDVFLFTGTIRDNILCGNPNATDEEMIAAAKKARIHDF
                     VETLPDGYDTYIGERGVKLSGGQKQRISISRIFLKNPPIIILDEATSALDNVTEREIQ
                     ESLEELSKDRTNLVVAHRLTTIKNADEIIVLTDKGIEERGTHEELVNKNGVYSRLHNN
                     "
     gene            2265..3452
                     /locus_tag="KPD58_002967"
     CDS             2265..3452
                     /locus_tag="KPD58_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266700.1"
                     /translation="MEAITAFCLVLVSLTIGDIVSAKTKAFVPSVFVSAIIFIVGFWT
                     FFPENIVDLAALGTPLAQLGMLLLITHMGTMMSIKELAGQWKTIVIALAGIVGICVGA
                     LALGTVVFGWDTAVIATPPLTGGLVASIMMADAATAKGLASLSVLAILMYVAQGFAGY
                     PITALMLKKEGKRLLSDFRSGKVTVNTDEEKVKDLPEQKSRFKIIPDLPEKYDTTYMI
                     ILRLGFVAWLAVQFTNLTNEVVSRYVICLIFGVIASELGIVDRKPLNKSGSFGWLMTS
                     LMAFIFAGLAKATPAMLVEMAVPLVGIIIFGVIGMGIFSIIVGKLLGESKEMAFSVSL
                     TSLYGFPPNYILTEEAVKALAETPEEKEFLMDKMLPKMLVGGFTTVTIASVVIAGIFV
                     NLI"
     gene            3512..4675
                     /locus_tag="KPD58_002968"
     CDS             3512..4675
                     /locus_tag="KPD58_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0266701.1"
                     /translation="MDIKEITKSYKDYVIKLRREFHENPEKSMEEVRTSKRVKEELDK
                     IGIPYVSAGGTGVIATIKGANPGKTVALRGDMDALQVVECTDVEYKSKNEGLMHACGH
                     DGHTSMLLGAAKVLNDIKDSINGTVKLFFQPGEEVGKGARAMIQDGAMEGVDSVFGIH
                     LWTDVESGTISVEEGPRMASADFFKITVKGRGGHGSLPHQGVDAVLASSAIVMNLQSM
                     VSREVSPLEPLVVSVGVLNSGTRFNVIASEAILEGTIRLFNPELRKQIPRILERIAKS
                     TAEAYRADAELEYGYLTPAVINDKECSKIATDAAIKLFGEDCITLFEKVTGAEDLAEF
                     MNIAPGALAFVGARNESKGACYPHHHGCFNIDEDALEIGTALYVQYAVDFLNK"
     gene            4826..5629
                     /gene="zupT"
                     /locus_tag="KPD58_002969"
     CDS             4826..5629
                     /gene="zupT"
                     /locus_tag="KPD58_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HBG0266702.1"
                     /translation="MNNVIFAFLITLLAGLSTGIGSCIAFFAKKTNTKFLSISLGFSA
                     GVMIYVSMIEIFPKAQDALTKSMGEKLGSWSTVIAFFIGMAIIAVIDKLIPQEENPHE
                     IKKMENIEEKNIKKNKSLLRTGIFTAMAIAIHNFPEGLATFISALDDVTIAIPIAIAI
                     AIHNIPEGISVSVPVYYATGDKKKAFYYSFLSGMSEPLGAIIGYVLLRNFLNDITLGI
                     VFAIVGGIMVFISLDELLPSAREYGEHHLSIYGLIAGMGVMAISLLLFK"
     gene            5724..6284
                     /locus_tag="KPD58_002970"
     CDS             5724..6284
                     /locus_tag="KPD58_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HBG0266703.1"
                     /translation="MSAMEKKIKFKFDWYSSPDKIEKWLEDIEAKGYNLYKINKFGGI
                     FYFHKGSPRKIKYTVDYYYDVENNYFDEHKSNGWNLVFRSIGSFMGTSAWYIWSKEYK
                     DIPPKLYSDSDKTNKVKSILFMNMFIFFMLVVIFIYLLNIEIRNLYSGYGASVTAIVL
                     QIILLILYAVQLYRRLMYYLRLKKFK"
BASE COUNT         2436 a          702 c         1223 g         2221 t
ORIGIN      
        1 aattttaaat atagagatat agctcttttt atgttgtttc aagtatacaa ctatatgata
       61 taaatcttca attttatgaa aaagattgtt attactttgt aagtttataa aagatttaaa
      121 gttattgtta tgttatacta atttatgggt attttttaga agtactaata gaagtactaa
      181 taaaggaggg atttaatact tatgataaaa caatttgtca aatattataa gccatataag
      241 aaaattttta cactagacct tatagcagct tttctatttt ccttatgtga tttagtttat
      301 cctatgataa ctagaaatat aatggatgac gttgttccaa ataaaaactt aagaatgtta
      361 gtagtttttg cagtagcact tatacttata tttatagcaa aagcagggct taactatttt
      421 atgcagtatt ggggacatgt tattggtgta gatatgcaag ctgatatgag aaatgaggta
      481 tttacacact tacaaagact tccaaatacg tattttgata ataacaaatc tggagtaact
      541 atgtctagaa tagtaaatga tttaatggat ataacagaac ttgcacatca tgggcctgaa
      601 gatttattca tttctatagt aatgttggtt ggttcattct ttatacttat agatataaat
      661 attcctctta cgcttataat atttgctata ttaccgttta taatttggtt tgcaatagct
      721 aaaaaagata agatgaatat tgcttttatg aagagtagag ttactatagg tgatgtaaat
      781 gctactttag aaaatagtat agcaggtatg aaggtaacta agtctttttg tactgagaaa
      841 gaagagttaa ataagtttgt aagaagcaat aaattattta gaagagcaag acaagattct
      901 tataaggtaa tggcagaata ttactctggt atgaatttat acatggatat cttagagtgg
      961 gtagttgtaa tagctggtgg ttattttact tatataggaa agattacttt gggtgatttt
     1021 gctgcttata tactatatgt aaaaatgttt atacaaccta tgaaaaaatt aataaacttt
     1081 acagagcaat atcaaaatgg tatgacaggt tttaaaagat tcatagagat aatggaacaa
     1141 gaccatcaaa aagaagctaa aaatcctatt gaacttgaaa atgtaaaagg tgacattgag
     1201 atagaaaata tatctttcac atatgaagat aagactcaag ttttggataa tttaagttta
     1261 tctataaaag ctggtaagac tatagcatta gttggacctt ctggaggagg aaagactaca
     1321 ttatgtaatc tattgccaag attttatgaa tttgataagg gtgatataaa aattgatgga
     1381 aagagtataa aagatgttag tttaaaatca ttgagaaaaa atataggtat agttcaacaa
     1441 gatgtatttt tatttacagg aactataaga gataatattc tttgtgggaa ccctaatgct
     1501 acagatgaag aaatgatagc tgctgctaaa aaagctagaa tacatgactt tgtagagact
     1561 ttgccagatg gatatgatac ttatatagga gaaagaggcg taaaattatc aggtgggcaa
     1621 aaacaaagaa tttcaatttc aagaatcttt ttaaagaatc ctccaataat aatattggat
     1681 gaggctacat ctgctttaga caatgtaaca gaaagagaaa ttcaagagtc tttagaggaa
     1741 ttaagtaaag atagaacaaa tttagttgtt gcacatagat tgactactat aaaaaatgct
     1801 gatgaaatca tagtattgac agataaagga attgaagaaa gaggtactca tgaagagcta
     1861 gtaaacaaaa atggagtata tagtaggtta cacaataact agtatacaaa ttttacacca
     1921 tagctaacat acacattaaa cttacaaaca taggatatat taagattcat aaagacaatt
     1981 cattttatat ttataagatg ttttatgaat aaaatgtatc ctatgtttta ttttttgcaa
     2041 attacattta attctctttg taagaataga gttaaatagc caatggtcgg gataacgttt
     2101 ttacaaaaag aataatttag aaaactaact ttaatttatg gaatttatcg aattttatga
     2161 aaaaaatcga attttgactt gaaatgtttt tcaaaagtag taaaataatg gataactata
     2221 catacatttt ataaaaatat atttattatg aggggggatt ttgtatggag gcaataacag
     2281 ctttttgtct agttttagta tcactgacga taggagatat agtttcagca aaaactaaag
     2341 cttttgttcc gtcggttttt gtatcagcaa ttatatttat agtaggattt tggacttttt
     2401 tcccagaaaa tattgtggac ttagccgctt taggaacacc attagctcaa ttaggtatgc
     2461 ttttgttaat aactcatatg ggaactatga tgagtattaa ggaattagca ggtcaatgga
     2521 aaacaatagt aatagccttg gcaggaatag taggaatatg tgtaggtgct ttggcattag
     2581 ggacagtagt atttggatgg gatacagcag taatagctac acctcctctt actggagggc
     2641 tagttgcatc tataatgatg gcagatgctg ctacagcaaa aggtcttgca agtttatcag
     2701 tacttgcaat tttgatgtat gttgcacaag gatttgcggg ttatcctatt acagcattaa
     2761 tgcttaaaaa agaaggaaaa agacttttat cagactttag aagtggaaaa gtaactgtaa
     2821 atacagatga agaaaaagtt aaggatttac ctgaacaaaa aagcagattt aagataattc
     2881 cagacttacc agaaaaatat gatacgacat atatgataat acttagatta ggatttgtag
     2941 catggttagc agtacaattt acaaatctta caaatgaagt tgtatcaaga tatgttatat
     3001 gtttaatatt tggtgtaata gcatcagaac ttggaatagt tgatagaaaa ccacttaata
     3061 aatctggttc atttggatgg ttaatgacat cattgatggc ttttatattt gcagggttag
     3121 caaaagctac accagcaatg cttgttgaaa tggcagttcc tcttgtagga atcataatat
     3181 ttggtgtaat aggtatggga atattctcta ttatagtagg taagctttta ggtgaaagta
     3241 aagaaatggc tttttcagtt tcgcttacat cactttatgg ttttccacca aactatatat
     3301 taactgaaga ggcagtaaag gctttggctg aaacaccaga agaaaaagaa tttttaatgg
     3361 ataagatgct tccaaagatg cttgttggag gatttactac agttacaata gcttctgtag
     3421 taatagcggg tatctttgta aatctaatat agtatttgag tgataaaaag tattttaaat
     3481 aagaagaatg tgtactactt ggaggtatgg aatggatatt aaagaaataa caaagagtta
     3541 taaagactat gtaattaagc ttagaaggga atttcatgaa aatcctgaga aaagtatgga
     3601 agaagttaga acatcaaaga gagttaaaga agaacttgat aaaataggga taccatatgt
     3661 atcagctgga ggtacaggtg taattgcaac tataaaagga gctaatccag gaaagactgt
     3721 agcacttagg ggtgatatgg atgctctaca agttgttgaa tgcactgatg tagaatacaa
     3781 atctaaaaat gaaggactta tgcatgcttg tggtcatgat ggtcacacat caatgcttct
     3841 aggggcagca aaagtgttaa atgatattaa ggatagtatc aatggaactg taaaattatt
     3901 ttttcagcca ggagaagaag ttggtaaagg tgcaagagct atgattcaag atggtgctat
     3961 ggaaggtgta gatagtgtgt ttggaataca tctttggact gatgtagaat ctgggaccat
     4021 atcggtagaa gaaggaccaa gaatggcttc tgctgatttc tttaaaatta ctgtaaaagg
     4081 tagaggtggt catggttcac ttcctcatca aggtgttgat gcagtgcttg caagttcagc
     4141 aatagttatg aatctacaat ctatggttag tagagaagta agtcctctgg aaccattagt
     4201 tgtgagtgtt ggagttttaa attctggaac tagatttaat gtaattgcga gtgaagctat
     4261 cttagaaggt acaataaggc tatttaatcc agagcttaga aagcaaattc ctagaatttt
     4321 agaacgtata gcaaaatcta cagcagaagc atatagagca gatgctgaat tagagtatgg
     4381 gtacttaaca ccagcagtta taaatgacaa agaatgttca aagatagcta cagatgcagc
     4441 aattaagcta tttggagaag actgtataac actatttgaa aaagtaacag gagcagaaga
     4501 tttagctgag tttatgaata tagcacctgg tgcactagca tttgtaggag ctagaaatga
     4561 gtcaaaaggt gcttgttatc ctcatcatca tggatgcttt aatatagatg aagatgctct
     4621 agaaattgga actgctttat atgtgcaata tgcagtggat ttcttaaata aataatttta
     4681 atatactata aagaaaaagc agagactaag tttatatctc tgctttttta ttgtgtatat
     4741 tttttataaa tattgactat ataaaatata tatgatagca taaagaaaat gataatcaat
     4801 atcaattaaa aagaaggtga tttatttgaa taatgtaata tttgcatttt tgataacatt
     4861 attggctggt ttatctactg gtataggaag ttgtatagca ttttttgcta aaaaaacaaa
     4921 tactaagttt ctttctataa gcttaggatt ttctgctggt gttatgattt atgtttctat
     4981 gatagaaatt tttcctaaag cacaagatgc tttgacaaaa tctatgggag agaaattagg
     5041 aagttggtca actgtaatag ctttttttat aggtatggct atcattgctg ttattgataa
     5101 gctaattcca caagaagaaa atccacatga aataaagaaa atggagaata tagaagaaaa
     5161 gaatatcaaa aaaaataagt ctcttttaag aacaggtata tttacagcaa tggctatagc
     5221 aattcacaat tttcctgaag gattagctac atttatatca gctcttgatg atgttacaat
     5281 agctattccg atagcaattg caattgcaat acacaatata ccagaaggta tatctgtatc
     5341 tgtaccagtt tattatgcca caggagataa aaagaaagct ttttattatt cctttttatc
     5401 tggtatgtct gagcctttag gagcgattat tggatatgta cttcttagaa attttttaaa
     5461 tgatataact cttggaattg tatttgcaat tgtaggagga attatggtat tcatatcact
     5521 agatgaatta ttgccatcag ctagagagta cggggaacat catttatcta tttatggatt
     5581 gattgcagga atgggtgtta tggctattag tttattacta tttaaataac ttttaaatat
     5641 atgattgtaa cctaaaatta gtatcaaaat gatataatta tttaaataga tttttaagct
     5701 atttaaaaat atcattttgg agaatgagtg ctatggaaaa gaaaataaaa ttcaaatttg
     5761 actggtatag ttcacctgat aagatagaga aatggttaga agatattgaa gctaaaggat
     5821 ataatttata taagataaat aaatttggag gaatatttta ttttcataag ggaagtccaa
     5881 gaaaaataaa atacactgtt gattactact atgatgtaga gaataattat tttgatgaac
     5941 ataaatcaaa tggctggaat cttgtattta ggagtattgg tagttttatg ggtacatctg
     6001 catggtatat atggtcgaaa gaatacaaag acatacctcc taaattatat agtgattctg
     6061 ataaaacaaa taaagtaaag tcaattttat ttatgaatat gtttatattc tttatgttag
     6121 tagtaatatt tatatattta ttgaatattg aaataagaaa tttatatagt ggttatggag
     6181 catctgtaac tgctattgta ctacagatta tattgttgat tttatatgca gtacaattat
     6241 ataggagatt aatgtattat ttaaggctta aaaagtttaa ataaataaaa gaaggtgtcc
     6301 taaaattatt tttttaatat tttaagacat cttcttttgt cagtaagtac tgtttttaat
     6361 tatagtaatt tctgtcttta aattgaaata tcaaataata gttgtatggg attgagtatt
     6421 gataaaaata atttaaaaat gttatcctaa agtatcagat atacaaaatt atctttaaat
     6481 atattataaa tattttaatt gtattaacta gaaattatgt aaggaggcat tttatgatta
     6541 agatagcagt ttgtgaagat gaaaaagaaa cacaactttt aa
//
LOCUS       DAELLV010000196         6545 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.196, whole genome shotgun sequence.
ACCESSION   DAELLV010000196 DAELLV010000000
VERSION     DAELLV010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6545
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.196"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            365..1639
                     /locus_tag="KPD58_002971"
     CDS             365..1639
                     /locus_tag="KPD58_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBG0266704.1"
                     /translation="MQFMDVSKYIEGANLKVKTKSGETVKRIYFNNSATPLVLKNVVD
                     NLNCEIPLLTYINAPGIISEKNTLKYENVRSTILKLIDGDEEKDSVIYVKNATEGINF
                     LAKFFKDENPDKFVITTAMEHMANYLPFKVNFPTKVVGITEKGELDLCQLENILKNYA
                     GNVSLVTVTGASNVTGITTPIYEIARIAHKYGAKILVDIVQVIQHKPFSMMPHECDEH
                     IDFVVFSAHKCYSPLNGGALVGPKKFFEKFNPVLYGSGSTKFVNDNKILYANVPQRFE
                     AGYPEYFGTLAMGKALNTLNKIGLNRISKYERELFLYAKEEMSKIPNIIIYGDKSNNV
                     IIPNISFNISNMYYKDVAKYLVDNFGIETGAGVVGADIYVQKLLGISPKEAYVRFMRE
                     NPVGLVRISLGMYNTFDEVDSFIHAIKTLAFK"
     gene            complement(1846..2163)
                     /gene="trxA"
                     /locus_tag="KPD58_002972"
     CDS             complement(1846..2163)
                     /gene="trxA"
                     /locus_tag="KPD58_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBG0266705.1"
                     /translation="MVKIVNNNEFINEVENKDGLVVVDFFATWCGPCKMLSPIYEALG
                     NEMVEKANFLKVDIDQSIELAQKFEVSTVPTMLIFKDGKPVDRLIGFMPKENLKNKIE
                     SYM"
     gene            2430..3182
                     /locus_tag="KPD58_002973"
     CDS             2430..3182
                     /locus_tag="KPD58_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HBG0266706.1"
                     /translation="MDNIINELQNIGFTKYESQIYISLLKESPLTGYEISKLSGVPQS
                     KVYENITKLLNNNIIINVGTDPIKYIPINPNELLKNKKKEFDNSMDNLKNSLQILNKS
                     KSVEYVLNIKGDKNILKKAIEMIKNAKQEIIISCPYEEILELKKELLIAYKKNINIEI
                     LLLENKSLKGLDNIHIHGGNKTTLENKMLYKGIILIVDNDEILTGNLSEGSEAISIWT
                     KNSNILYIGVQYIKHEIYISEQIKGGYKNEQN"
     gene            3169..4050
                     /locus_tag="KPD58_002974"
     CDS             3169..4050
                     /locus_tag="KPD58_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBG0266707.1"
                     /translation="MNKIKIKQVDAFTTIPFGGNPAGVVTDTSNISDEIKQNVAKEMN
                     LSETAFVSESNIADFKVQFFTPGLEVDLCGHATIGTFHALLEEGKLNTNKSIFYQETK
                     AGVLSVELKKINNENIFMMEQNTPEFENLDNCRKELAQMIGVHEDRLLEYPIIKVNTG
                     LWWLVFGLKSLDDLKNISPDLNKIKKFSGDNNIIGITPFCIETIDKKCDYHLRSIAPY
                     VGVTEDPVCGTGNACVSSYIVHHNILDKTSFIGEQGNFINRPGKVYVNITRENNNITS
                     VKIGGSAYTVLSGEIMY"
     gene            complement(4276..5631)
                     /locus_tag="KPD58_002975"
     CDS             complement(4276..5631)
                     /locus_tag="KPD58_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBG0266708.1"
                     /translation="MSSTVKKKGKFPMGFYICSTTFSFERAAYYASKYLIYIFLTTAI
                     VHGGLGIDKGQAAIMQANLVAFTYLAPIIGGYISDRWIGARYTIPIGMLIMGVGYYLG
                     SIATTVSMVNAMIILVSIGTAFFKGNVSAVNGQLFDSQEELDTAFSVQYSFVNIGSFI
                     GTIAVGILYLKTFAKNGVLGFSQCFFIAAVLCVIGAIWFIYGWRFLGNAGKRPFKEGV
                     VAEKIEEKDKSPLTSMDKKRIWAIILISFFSVIFWVFWYLTYLAVYDYGAAFVNMNVG
                     GFDVPLAWFDSLNSLVCIVLGPVLGALWFKLASRPQGDMSLFKKTGLGLIFLGLAFLM
                     LVGAEFSRGVGAPETAKASILWIIMFGILLSLGEMLFSPLGNSFVSKYAPKKLLGVLM
                     GVWTFATFIAGKGYGYIYAFTLKFDMIKVYTIIPVILFVAAILLFLSDKTLSRLVEDD
                     K"
BASE COUNT         2516 a          942 c          825 g         2262 t
ORIGIN      
        1 cattatgttc taatcaattt ctttactatt tttacaactt tattagtcat attcataatt
       61 atttctaatt agtgtttaac attttatgta aaatttataa aaaaatgtat ataaattaaa
      121 ataagtgact atactaaaaa tatgatacgg ataaacaata agttcaacct atctccccca
      181 aaatcaagtg agctacagtt tcccccaact gtagctcatt ttcgtgtaat ttaataaaca
      241 tctcaacaag aattaattaa caaaacccct tataaaaaca taacatatat taaacaagct
      301 cttgattaaa acttgagctt ttttaataaa aaacagaata gtttattaga taaggggttt
      361 ttttatgcaa ttcatggatg tatctaaata cattgaaggt gcaaacctta aagttaaaac
      421 taaaagtggt gaaactgtta aaagaatata ttttaacaac tctgccactc cattagtttt
      481 aaaaaacgta gtggataact taaactgtga gattcctttg cttacttata ttaatgctcc
      541 aggaataatc tcagaaaaaa atactttaaa atatgaaaat gtacgctcta ctatcctcaa
      601 acttattgat ggagacgaag aaaaggattc tgttatttat gtcaaaaatg ctacagaagg
      661 aataaacttc ttagcaaaat tttttaaaga tgaaaatcca gataaatttg ttattaccac
      721 agcaatggag catatggcta attacctgcc ttttaaagtt aattttccta caaaagttgt
      781 tggaattaca gaaaaaggag aacttgattt atgtcaatta gagaatatac ttaaaaatta
      841 tgctggtaat gtatctctcg ttacagttac aggagctagt aatgtcacag gtataaccac
      901 tcctatatat gaaatagcaa gaatagctca taagtatggt gcaaagatat tggtggatat
      961 tgtgcaagta attcagcata aacctttttc aatgatgcca catgaatgtg atgagcatat
     1021 agattttgtt gtttttagtg ctcataaatg ttattctcca ctaaatggag gagctctggt
     1081 aggacctaaa aaattctttg aaaaatttaa tcctgtacta tatggctcag gttctaccaa
     1141 atttgtaaat gataacaaaa tactatatgc aaacgtaccc caacgttttg aagcaggata
     1201 cccagagtat tttggaactc ttgcaatggg aaaagcactg aacactttaa ataaaattgg
     1261 tctaaacaga atttcaaaat acgaaagaga attattttta tatgctaaag aagaaatgag
     1321 taaaatacct aatataataa tatatggaga taaatcaaat aatgtaatta tcccaaatat
     1381 atcttttaat ataagtaata tgtattacaa agatgtagca aaatatttag ttgataactt
     1441 tggaatagaa actggagctg gagtagtagg tgcagatatt tatgtacaaa aattattagg
     1501 tataagtcca aaagaagcat atgtcagatt tatgagggaa aatcctgtag gtcttgttag
     1561 gataagctta ggaatgtaca atacttttga tgaggtggat agctttatac atgctattaa
     1621 aactttggct tttaaataag ttttttctat tcttttttat caataaatag agagtagttt
     1681 tgttttcaca aaaaagcaaa ttatatcaac agtcaatttc attagcaatt agacactcat
     1741 ttgtaactat acaaactatt ttacactata caaacaaaaa actcattaca atgagttttt
     1801 ttgtttatat atgagattta ttttaattac ttcaatttac tttaattaca tatatgattc
     1861 aattttgttt tttaaattct ccttaggcat aaaacctatt aatctatcta caggttttcc
     1921 atccttaaat attagcatag ttggtactgt agatacttca aatttctgag ctaattctat
     1981 actttggtct atgtccacct tcaagaagtt tgctttttca accatctcat ttcctaaagc
     2041 ttcatatatt ggtgatagca tcttacaagg tccacaccaa gtcgcaaaga aatctacaac
     2101 tactaaacca tctttatttt ctacttcatt tataaattca ttattattta ctatttttac
     2161 cataatatac atctccttta ttatcttctt gatattaata tactacgacc tatataacag
     2221 ttcaattagg cacttttttg taaccgccta tttttaatag atttaatatt atgataaata
     2281 gatttataag attaaatatt tataagacta aatatttcta atatatttta tttttttctt
     2341 aatatagttg acattttttc ccttaaatta taaaatcaaa ttaataacta cataggtagt
     2401 tactaatctt ttatcaaagg agtttctata tggataatat tattaatgaa ttacaaaata
     2461 taggatttac taaatacgaa tctcaaatat acatatcatt attaaaggaa agccctttaa
     2521 caggatatga aataagtaaa ttatcaggtg taccacaatc aaaagtctat gaaaatataa
     2581 caaagctatt aaacaataat ataattatca atgttggaac tgaccctatt aagtacatac
     2641 caataaaccc taatgaatta ttaaaaaata agaaaaaaga atttgacaat tctatggata
     2701 atctaaagaa tagcttacag atattaaata aaagtaaaag tgtagaatac gtattaaaca
     2761 taaaaggaga taaaaatata ttaaaaaaag caatagaaat gataaaaaat gcaaaacaag
     2821 aaattataat ttcttgccct tatgaagaaa ttcttgaatt aaaaaaagaa ctcctaattg
     2881 cttacaaaaa aaatattaat attgaaatac tacttttaga aaacaaaagt ctgaaaggtc
     2941 ttgataatat tcatattcat ggaggtaata aaactacact tgaaaataaa atgctttaca
     3001 aaggaattat tttgattgta gataatgatg aaatattgac aggaaactta tcagaaggca
     3061 gtgaggccat ttcaatttgg acaaaaaact ctaacatttt atatattgga gttcaataca
     3121 taaaacatga aatttatatt tcagaacaaa taaaaggagg ttataaaaat gaacaaaatt
     3181 aagattaaac aagttgatgc ttttacaact ataccttttg gtggaaatcc tgctggtgtc
     3241 gttactgata cctctaacat ttctgatgaa attaagcaga atgtggctaa agaaatgaat
     3301 ttatcagaaa ctgcatttgt ttcagagtca aatattgcag attttaaagt acaatttttc
     3361 acaccaggtt tagaagttga tttatgtggt catgcaacaa taggtacttt ccatgcactt
     3421 ttggaagaag ggaagcttaa tactaataaa agtatatttt atcaagaaac aaaagctggt
     3481 gtattatctg tagagcttaa gaaaataaat aatgaaaata tatttatgat ggaacagaat
     3541 acacctgaat ttgaaaattt agataattgc agaaaagaac ttgcacaaat gataggtgtg
     3601 catgaagata gacttcttga atatcctatt ataaaggtaa atactggact ttggtggctt
     3661 gtatttggtc taaaatcttt agatgattta aaaaatatct ctccagattt aaataaaatc
     3721 aaaaaattta gtggtgataa taatataatt ggtataactc cattttgtat agaaactata
     3781 gataaaaagt gtgactacca tttaagatct atagcaccat atgttggagt tacagaagac
     3841 cctgtatgtg gaactgggaa cgcttgcgta tcatcttata tagtacatca caatatttta
     3901 gataaaacta gttttattgg tgagcaaggt aactttataa acagacctgg aaaggtatat
     3961 gtaaatataa ctagagaaaa caataacata acttctgtaa aaattggagg aagtgcttat
     4021 actgtcttaa gtggtgaaat tatgtattaa tcccatacct aataaaaaca aataaaaata
     4081 ctagctaact aaagctagta tttttatttg ttatttttat ttaataatca tactctaaca
     4141 aatattataa ttatatatat cataatattg tatttagaag taaaatttta tttttattat
     4201 taattagtta taaatcaatc taatacaaca taattacagt agattaacct ataaaacata
     4261 ataattgctt actgattatt tatcgtcctc aacaagtcta cttaatgtct tatcagataa
     4321 gaataataat atcgctgcaa caaatagtat tactggaatt atagtatata ctttaatcat
     4381 atcaaattta agagtaaatg catatatata tccatatcct ttaccagcta taaaagtagc
     4441 aaatgtccat actcccataa gaacacctag aagcttctta ggtgcatact tacttacaaa
     4501 agagttacca agtggtgaga ataacatttc accaagactt aaaagaatac caaacatgat
     4561 tatccatagt atactagctt tagcagtttc aggagctcca actcctcttg aaaattctgc
     4621 accaacaagc attaagaatg caagacctaa gaatattaat ccaagacctg tctttttaaa
     4681 taaactcata tctccttgtg gtctactagc aagtttaaac cataatgctc caagaactgg
     4741 tcctaatact atacatacaa gtgaatttaa tgagtcaaac caagcaagag gaacatcaaa
     4801 tcctccaaca ttcatgttta caaatgctgc accataatca taaactgcaa gataagttag
     4861 ataccagaat acccagaaaa ttacagagaa gaatgatata agtataatag cccaaattct
     4921 cttcttatcc atgcttgtaa gaggagactt atctttttct tcaatttttt cagcaactac
     4981 accttcttta aaaggtctct ttcctgcatt cccaaggaat ctccatccat atatgaacca
     5041 tattgcacct attacacaaa gtacagctgc tataaagaaa cattgagaaa atccaagtac
     5101 accatttttt gcaaatgttt taaggtaaag aataccaact gctatagtac caataaaaga
     5161 acctatatta acaaaagaat attgtactga aaatgctgta tcaagttctt cttgactatc
     5221 aaaaagctgt ccatttaccg cagatacgtt acctttaaag aatgcagtac caattgaaac
     5281 taagattatc attgcattta ccatagaaac tgtagttgca atagaaccaa gatagtatcc
     5341 tacccccatg ataagcatac ctataggtat agtatatcta gctcctatcc atctatctga
     5401 tatatacccc ccaattatag gtgcaaggta tgtgaatgca acaaggttgg cttgcattat
     5461 agcagcttgt cctttatcta ttccaagacc accatgaact atagctgttg taaggaatat
     5521 atatattaag tactttgaag cataatatgc tgctctttca aatgagaaag ttgttgagca
     5581 gatataaaat cccataggaa atttaccctt cttttttact gttgagctca taattttaaa
     5641 tcctttctta attgtatttt tattttttac gttttgtcca aattctttct tttatgaaag
     5701 atataatatt attattttgt aaaacaattt gtaattatta ttaagataat tgcgcattta
     5761 tttgtagagt acatattctc atggaataaa attaatacac ttcttaatca taaatttaaa
     5821 ttatataaat tcactatttt cagatatagt tataaattaa tatattctca tctaatacca
     5881 tgaaaaaacc ttcgccatat gcaattctta ttttttacgt gatagtattt tacaaactaa
     5941 aatccccctt ttgtagcaac ttatgtaatt acattttagt gcttttttaa aggagtctcg
     6001 aataaagcaa catatatttt gttcaaataa tgtttttatt tattttttaa atttatatat
     6061 tacttcaccc aaccaattct caacttaaat aactaaacta ctatctattc acagattagg
     6121 cttatttctt acacattagt ttataataaa cactgtgaat ctaaacataa aaactgttat
     6181 tttaaagtaa ttacccatat attcgctttg atactattta ttgaggctat actctttatg
     6241 tataatatat tatttataaa atacctcaaa atatattatt cataaaatac ctcaaactga
     6301 aaactttttc acgaatattt atttttcacc tccccttttt tatttcacca tgtaaatcgt
     6361 accataaata tgcacttata tgcaagaaaa aaatctatta atcgacagta aataattaga
     6421 aaaattcaaa ttatatttat caattatatt atataaaata tttcctatga aaatataaat
     6481 taaattaatg ttcagaatag tttttagata tgaaatcttg atttttttat tactttactt
     6541 atttg
//
LOCUS       DAELLV010000197         6524 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.197, whole genome shotgun sequence.
ACCESSION   DAELLV010000197 DAELLV010000000
VERSION     DAELLV010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6524
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.197"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            187..1431
                     /locus_tag="KPD58_002976"
     CDS             187..1431
                     /locus_tag="KPD58_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBG0266709.1"
                     /translation="MFENLKKADPEIYESMKRELKRQQRNIELIASENIVSVPVMETM
                     GSHLTNKYAEGYSGKRYYGGCEYIDEVETLAIDRIKKIFGAEHANVQPHSGANANIGV
                     YFAMLKPGDVVMGMNLSQGGHLTHGAPVNISGQYYKFYEYGIDKDSGLIDFDEVRKIA
                     HEVKPKMIVAGASAYPREIDFKKFREIADEVGALLMVDMAHIAGLVAAGLHQNPCEVA
                     DFVTTTTHKTLRGPRGGVILCKEKYAKDIDKAIFPGIQGGPLEHIIASKAVCFKEALS
                     DEFKEYQVQVAKNAKALAEELIKRDFKLISGGTDNHLILLDLTNKNITGKAAEKRLDD
                     AYITANKNTIPFDPNGALVTSGIRLGTPAVTTRGMKEEDMAIIAEAIDLCLTYDEESK
                     ARTLVVGLTEKYPLYEEYNIMD"
     gene            1754..2851
                     /locus_tag="KPD58_002977"
     CDS             1754..2851
                     /locus_tag="KPD58_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-diphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBG0266710.1"
                     /translation="MKVLILTGKFGMGHYSASNSLSEDIKAKFDNSEIIIKDIFEYIM
                     PNYSDKMYKTFSILVNRGSSLYNLFYKCAENGKKDIKFTFSDYFLNKLDTLLHEVQPT
                     VVISTFPFCSQLVSRYKEKYNSNLPLITCITDISSHSEWISKNTDCYLVASKSTKEEL
                     VFKGIDESKIKVNGIPVKKEFKRIEHVNHSTKKNILIMGGGLGLLPKSEQFYKELNSL
                     EGVKTTVITGNNKKMYYKLYGRYENIEVVGYTNEVYKYMKDSDLIISKPGGITLFETI
                     YSELPILAFNPFLQQEIDNASFILNNEIGRILGKNKKYYVDEIKDLIYDDATLKEMSS
                     NMKELKKQFDNNTLENILFSLDEQGACRECM"
     gene            2842..3540
                     /locus_tag="KPD58_002978"
     CDS             2842..3540
                     /locus_tag="KPD58_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG0266711.1"
                     /translation="MYVVGLIIVVALIFLVHSIIPTYYNKLLNKKVLKNMAGENEIAL
                     TFDDGPDKRYTEKLLDVLKENDIQAMFFVVAKNAEKEPEIIKRMLRENHIVGLHSLEH
                     RNAWLYSYSYVKKDFIESTNIMKNLGVDVNYYRPPWGHTNIFSNSFVKKYNLKMTLWD
                     VMAEDWEKDSTVDIIINKLMSRTKENSIICLHDAGENSGGAVGAPERTIEALKIAIPK
                     LKASGLKFVTPERM"
     gene            3543..4607
                     /locus_tag="KPD58_002979"
     CDS             3543..4607
                     /locus_tag="KPD58_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBG0266712.1"
                     /translation="MISKTEKKNKCLGSVAFLVLLMGITGYFVFRGQSVESLIKSLKG
                     ASPMFILIGFVMMFIYVACEGINIYLGMKALNQKTTLLKCMGYAFIGFYFSSITPSAS
                     GGQPAQVYYMKKDDINISYSSLILLVIVVIHQVVILAYSGIMFIMEREFILNNVSGMN
                     ILLIYGVITNVALVIGVIAIIFSKKLVNNFIISITNLLGKLRIIKDVESSRKVIISQI
                     EEYVKGAQYIKQNPKLVVQILVITIVQITAMFLVPFFVYKAFHLSTYTVFEILAIQSL
                     LNIAVSSLPLPGAVGASENSFMTLFKIFFPGHLLVPAMLLSRGISFYAFVAISGLICI
                     VVHVKSSEKVKAVKKVAYVR"
     gene            4790..5494
                     /locus_tag="KPD58_002980"
     CDS             4790..5494
                     /locus_tag="KPD58_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0266713.1"
                     /translation="MNKEIPKKILVADDNPEIREIVEILLTGEGYEVIMATNGEEAVN
                     LVDSTIDLIILDVVMPVKTGFVACNEIREKTTAPILFLTAKTQDSDKVIGFSAGGDDY
                     LSKPFSYSELISRVKSLLRRYYIYQGKEKEREPNILKTKELCVNLDTQDVTLNGEPVF
                     LTTIEYSILILMLKNRKKVFSSENLYETIWDEQYFYTANNTIMVHIRNLRKKLEKDQK
                     NPQYIKTAWGKGYYID"
     gene            5487..>6524
                     /locus_tag="KPD58_002981"
     CDS             5487..>6524
                     /locus_tag="KPD58_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0266714.1"
                     /translation="MINKIKPSKLGVILLIYVVISAAVAFILNIPFYSAVESIVKDVS
                     EGKVYFKREVNQLMDNFQTYVNNNGIRADDGDDIAKWNSDNWFVSMKIYKGNKVFYDT
                     LYYPNEVPDKSEENEFYSSPMARNYEIQFSDIKANIVVVALFRFRFQNFIEAISITFM
                     ALTFIITFLLLFSRKIKYLRKIEMGIKIVESGSLKYRIPVKGNDELSSLASSINEMSH
                     TLENQLIKEREMKNKNKDIVTSISHDVRTPLTSVICYLDLIKDHKYNSPEQLEQYIKN
                     VRSKAYQIKDLTDDLFTHFVNSGEEVSKGYEKINANEFIFQLLLDSSYTLEEKGFHIT
                     IENELYNQYHIY"
BASE COUNT         2555 a          681 c         1127 g         2161 t
ORIGIN      
        1 gacaatatta tttattatat tttaacttaa aaataaagaa agtaactatg ttaaatatgg
       61 aaaaatggca aaacacttga ctattaattg gtaaaggact ataataataa attatcatat
      121 tgataaaaac gaaaattatg aaatgtctgt aaaaataaaa tttaaataat aactaggagg
      181 agtaatatgt ttgagaactt gaaaaaagcg gaccctgaga tttatgaaag tatgaaaaga
      241 gagttaaaaa gacaacaaag aaatattgaa ttaatagcat ctgaaaacat agtatcagtt
      301 ccagtaatgg aaactatggg aagtcatctt acaaataagt atgcagaagg ttactcaggc
      361 aaaagatatt atggtggatg tgaatacata gatgaagttg aaactcttgc aatagataga
      421 atcaaaaaaa tatttggagc agaacatgct aatgttcaac ctcactctgg agcaaatgca
      481 aatattggtg tatattttgc tatgttaaaa ccaggtgatg ttgttatggg aatgaattta
      541 tctcaaggtg gacatttaac tcatggtgct ccagttaata tttctggtca atattataaa
      601 ttttatgaat atggtataga taaagacagt ggacttatag attttgatga agttagaaag
      661 atagcacatg aagtaaaacc taaaatgata gttgcaggag caagtgcata tcctagagaa
      721 atagatttta aaaagtttag agagatagct gatgaggttg gagcattgct tatggtagat
      781 atggctcata tagcaggtct tgtggctgct ggtcttcacc aaaatccatg tgaagtggct
      841 gattttgtta caacaacaac tcacaaaact ttaagaggac ctcgtggggg tgttattctt
      901 tgtaaagaaa aatatgctaa agacatagac aaagcaatat tccctggtat tcaaggtgga
      961 cctcttgaac atataattgc ttctaaggca gtttgcttta aagaagcttt aagtgatgag
     1021 tttaaagaat atcaggtaca agttgctaaa aatgcaaaag ccttagctga agaacttatt
     1081 aaaagagatt ttaaattgat ttctggaggt acagataatc atttaatact attagatttg
     1141 actaataaaa atataactgg taaagcagca gaaaagagat tggatgatgc ttatataaca
     1201 gctaataaaa atacaattcc atttgaccca aatggtgctc ttgttacaag tggaataaga
     1261 cttggtactc cagctgtaac tactagaggt atgaaagaag aagatatggc aataatcgct
     1321 gaggctatag acctatgttt aacttatgat gaagaatcta aggctagaac attggttgtt
     1381 ggtttaactg aaaaatatcc tttatatgaa gaatataata ttatggatta attataaaga
     1441 acaactaaat aaaatacatt taaataatta caatgattgt gtatgtattt gaatatattt
     1501 agatataaaa aaggtgtctc aaaatgaact ttttagttta tgaggcactt ttttgtatgg
     1561 aatcgaacat atttgtatta tttaaataat aaaaaatatg gttatctcta tttttagatg
     1621 gtctctttgt attttttaat taaaacttta agatttctta agaattatac taggtttttc
     1681 taaagattta aagtctatac ttataagtat agtaatcata tgaaaagaaa agctttaacg
     1741 aggagggaat cttatgaagg tattaattct tacaggaaaa tttggtatgg gtcattactc
     1801 agcatcaaat tctttaagtg aagatataaa agcaaaattt gataattcag aaataataat
     1861 taaagatata tttgagtaca ttatgcctaa ctattcagat aaaatgtaca agactttttc
     1921 tattcttgta aatcggggaa gtagtcttta taatttattt tataaatgtg ctgaaaatgg
     1981 aaagaaagat ataaaattta ctttttcaga ttatttttta aataaattag atacattact
     2041 tcatgaagta caaccaacag tagttatttc aacttttcca ttttgttctc aattagtatc
     2101 aagatataag gaaaaatata actcaaatct tcctttaata acatgtataa ctgatataag
     2161 tagtcactca gaatggatta gtaaaaatac agactgttat ttagtagcta gtaaatctac
     2221 taaggaagaa cttgttttta aaggaataga tgaatctaag attaaagtta atggtattcc
     2281 agtaaaaaaa gaatttaaaa gaatagaaca tgtaaatcat tcaactaaaa aaaatattct
     2341 tataatgggt ggaggattag gattactgcc taaatcagaa caattttaca aagaattaaa
     2401 tagtttagaa ggagtaaaaa ctactgtaat aactggtaat aataaaaaaa tgtactataa
     2461 actatatggt agatatgaaa atatagaggt agtaggttac accaatgaag tatataaata
     2521 tatgaaagat tctgacctta ttatttctaa accaggtgga ataacactat ttgaaacaat
     2581 ttattcagaa cttccaatat tggcatttaa tccattttta caacaagaga tagataatgc
     2641 tagttttata ttaaataatg aaattgggag aatacttggt aaaaataaaa aatactatgt
     2701 agatgagata aaagatttaa tttatgatga tgctactctt aaagaaatga gcagtaacat
     2761 gaaagaatta aaaaaacaat ttgataataa taccttagag aatattttat tttcacttga
     2821 tgaacaagga gcgtgtagag aatgtatgta gtaggtttaa ttatcgttgt tgcattgatt
     2881 tttttagtcc attcaattat acctacatac tataataaac tattaaataa aaaggtatta
     2941 aaaaatatgg ctggagaaaa tgaaattgca cttacatttg atgatggtcc agataagaga
     3001 tatacagaaa aattactaga tgtgttaaaa gaaaatgata tacaagctat gttttttgta
     3061 gtagcaaaga atgctgaaaa agagcctgaa attataaaaa gaatgttgag agaaaatcat
     3121 atagttggtt tacattcatt ggaacacaga aatgcatggc tttatagtta tagttatgtt
     3181 aaaaaagatt ttattgaaag tacaaatata atgaaaaatt taggtgtaga tgtaaactac
     3241 tatcgtccac catggggtca tactaatata ttttcaaatt catttgttaa aaaatataat
     3301 ctaaaaatga ctttgtggga tgtaatggca gaagattggg agaaagatag tacagtagac
     3361 attattataa acaagttaat gagtagaaca aaagaaaatt ctataatttg tttacatgat
     3421 gcaggtgaaa attcaggtgg tgcagtaggt gcacctgagc gtactattga agctttaaag
     3481 atagctattc caaaacttaa agccagtggt ttaaagttcg taacaccaga aaggatgtag
     3541 ttatgataag taaaactgaa aagaaaaata aatgcctagg tagtgtagct tttttagttt
     3601 tactaatggg cataacagga tattttgttt ttagagggca gtcagttgaa tcattgataa
     3661 aatcattaaa aggtgcaagc cctatgttta tactaattgg atttgtaatg atgtttatat
     3721 atgttgcctg tgaaggtata aatatatact taggtatgaa agctttaaac caaaagacta
     3781 ctcttttaaa atgtatggga tatgctttta ttggatttta ttttagttct attacacctt
     3841 cagcatcagg tggtcaacca gcacaagttt attatatgaa aaaagacgat ataaatatat
     3901 catattcttc acttatacta ttagtcattg ttgttattca ccaagtagtt atactggcat
     3961 atagtggaat aatgttcatt atggaacgtg agtttatatt aaataatgta agtggtatga
     4021 acattttact aatatatggt gtaataacaa atgttgctct tgtcattgga gtaattgcaa
     4081 ttatattttc aaaaaaactt gtaaacaatt ttataatatc aataacaaat ttattaggta
     4141 agttaagaat aataaaagat gttgaaagtt caagaaaagt tataatatct caaattgaag
     4201 agtatgttaa aggtgctcaa tatataaagc agaatccaaa acttgtagtc caaattttag
     4261 tcataactat tgtacaaata acagctatgt ttttagttcc tttctttgta tacaaagctt
     4321 ttcatctatc aacatatact gtatttgaga tattagcgat acaatccttg cttaatattg
     4381 ctgtttcttc attaccattg ccaggggctg ttggagcttc tgaaaatagt tttatgacat
     4441 tatttaagat atttttccct ggtcacttat tagttccggc aatgttactt agtaggggga
     4501 taagttttta tgcatttgta gctataagtg gattgatatg tatagtagta catgtaaaat
     4561 cttctgaaaa agtaaaagca gtgaaaaaag ttgcttatgt aagataatat tatatagatt
     4621 atttttatta atatgtcaat ttgtgtgtat attttaatta agctttaaac atgtttacag
     4681 gagtagaaac tattaaaacg tgtgacattg ctaagctttt gctaataaag tataatattt
     4741 tatatagttt gaaatattta agaaatatag caaaaaaagg agcttggtga tgaataaaga
     4801 gattccaaaa aaaattttag tagctgatga taatcctgag attagggaaa tcgtagaaat
     4861 acttttaact ggtgaaggtt atgaagttat tatggcaacg aatggagaag aagctgtaaa
     4921 cttagtagat agtacgattg atttgattat tttggatgtt gtaatgcctg taaaaacagg
     4981 atttgttgca tgtaatgaaa ttagagaaaa aacgacagca ccaatattat ttttaactgc
     5041 aaaaactcaa gactctgata aagttattgg attttcagca ggaggagatg actatttatc
     5101 aaagcctttt tcatattctg aactaatttc aagggttaag tctctactta gacgttatta
     5161 tatataccag ggaaaggaaa aggaaagaga gccaaatata ttaaaaacaa aagagttatg
     5221 tgtcaattta gatactcaag atgttacact aaatggagaa cctgtgtttt tgacaactat
     5281 agaatatagt atattaattc ttatgttgaa aaatagaaaa aaagtttttt cgtctgaaaa
     5341 tttatatgaa actatttggg atgagcagta tttttataca gcaaataata caataatggt
     5401 tcatattaga aatttaagaa aaaagttaga aaaagaccaa aaaaatccac aatatataaa
     5461 aaccgcatgg ggaaaggggt attatattga ttaacaaaat taagccatct aagttgggag
     5521 ttatactatt gatatatgta gtaatctcag ctgcagtggc tttcatttta aatataccat
     5581 tttactctgc tgttgaatct atagtaaaag atgtatcaga agggaaagta tattttaaaa
     5641 gagaagtaaa tcaacttatg gataattttc aaacttatgt gaataacaat ggtataagag
     5701 cagatgatgg agatgatata gcaaaatgga atagtgataa ttggtttgtt tctatgaaga
     5761 tatataaagg aaataaggtt ttttatgata cattatacta tcctaatgaa gtacctgata
     5821 agagtgagga aaatgagttt tatagcagtc ccatggctag aaactatgag atacaatttt
     5881 ctgatattaa agctaatata gttgtagtag ctttatttag atttagattt caaaatttta
     5941 tagaagcaat ttctattaca tttatggcac ttacatttat tataacattt ttactgttgt
     6001 ttagtagaaa gataaagtac ctaaggaaaa tagaaatggg tataaagata gtagagagtg
     6061 gcagtttaaa gtatagaatc ccagtaaaag gtaatgatga attatcatca cttgcatcta
     6121 gtattaatga gatgagtcat acattggaga atcaacttat aaaagaaagg gagatgaaga
     6181 ataaaaataa agatatagta acttcgatat cacatgatgt tagaactcca ttaacgtcag
     6241 taatttgtta tttagatttg attaaagacc ataaatacaa tagtcctgag caattggaac
     6301 aatacataaa aaatgtaagg tctaaagcat atcaaataaa agatttaaca gatgaccttt
     6361 ttacgcattt tgttaattct ggagaggaag tttcaaaagg gtatgaaaaa ataaatgcta
     6421 atgaatttat ttttcaatta cttttagata gttcatatac attggaggag aaaggatttc
     6481 atataacaat agaaaatgaa ctttataacc aatatcatat ttat
//
LOCUS       DAELLV010000198         6490 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.198, whole genome shotgun sequence.
ACCESSION   DAELLV010000198 DAELLV010000000
VERSION     DAELLV010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6490
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.198"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            67..1809
                     /locus_tag="KPD58_002982"
     CDS             67..1809
                     /locus_tag="KPD58_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M3"
                     /protein_id="HBG0266715.1"
                     /translation="MVKSKKKIFVFLILIAVFIIFASYIRNYTNDTKKDSVRGTIPFE
                     KMEYKRPNIQSICENINKCNDKLLTAKTAKEQLNLFNEVDKIYQDFYSTLTIAKIRNN
                     IDSSDEFYSKEYKYLMSKSVDVDMSYKDFQDRFVTSKFSKELEKELGNDTFNYLKNIG
                     KLNSKEVEGLLKKEQDLVVKYEDLLAKSTVSIDGIEVDFEEALSRPNLSPEEYVKIYS
                     DYLKKYNPIFGNIFLELIQTRTEIASKQGFKNYIDYAYMNLNKDYSQEEAKKFRQDVK
                     DYIVPLYREISSKPSDSSIYIKVYKNRSFRKFDKVLEDISPKLKESFDYMKKYDLYDY
                     SSGKNKSPGGYTTYINKYKAPFLFNTWDNSFLGVTSFAHEFGHFYNYYNSINLNNKMQ
                     PSIDVCEVHSTSLEILFYKYFDDFFGKQSEAIKKEHLSIVLNTIIDACLYDEFQEIIY
                     KNPYMSLNDINKLFFDLEEEYGVSNNILREKNAPFWILVSHNFQVPFYYLSYGLASDV
                     SLQIWQLSQEDYRKAVDVYMDFLNQNTDAGFKDVVEKVNLQLPFQNGNLEEISSTLYD
                     YFGIDNPLELKNAS"
     gene            complement(1973..2167)
                     /locus_tag="KPD58_002983"
     CDS             complement(1973..2167)
                     /locus_tag="KPD58_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266716.1"
                     /translation="MKIITIGSSLITVLLFLSTMVCGFWIKNNKVTDASSIKFHMNSA
                     IFTGIFLLISTILLIIYIKK"
     gene            complement(2164..2745)
                     /locus_tag="KPD58_002984"
     CDS             complement(2164..2745)
                     /locus_tag="KPD58_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0266717.1"
                     /translation="MKSNDKDARERIIEVTLNLLNEVDDIEEITVRKIAERANVGVGL
                     INYHFKTKDNLLSTAIGDVMSNIIAELYDDSVYTLRPIEDLKNLLKKLCDTGLHYEKV
                     LPFVLNQCITNGDMQAELDIVPMLRKIFGNKKDEMSLRIIALQIILPIQISALSTESF
                     QLYSGINIKNKYERDKFIDILIENIIGEDVDVR"
     gene            2913..3731
                     /locus_tag="KPD58_002985"
     CDS             2913..3731
                     /locus_tag="KPD58_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0266718.1"
                     /translation="MFKIGDFSKLSKISIRMLRHYDEIGLLTPSHTNKTNGYRYYSAD
                     QLSTTNRIHALKDMGFGLYSIKEILTEYNDKESLIKYLNIHHSQVKEQLEDTQKKLLK
                     IETTIKRIGGNDIMKNYDVTIKNFAPKYMMTLRRVIPTYQDEGMLWHQAFLETKDQNV
                     QIEPPKYSKTVFYDTGYKEDYVDVEVQVAVSGKYKDTEHVKFKTVPSVTAATAIVNGN
                     FNQVADACEAIGNWISDNNYDVDGPMFNIYHVSPGNDSNPDNWVTEVCFPVKKK"
     gene            4465..5286
                     /locus_tag="KPD58_002986"
     CDS             4465..5286
                     /locus_tag="KPD58_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallohydrolase"
                     /protein_id="HBG0266719.1"
                     /translation="MKLKIVGSGGMSIIPSSFCKCKICEEARQKGGRYERLGPSLYID
                     DIKMLIDTPEDIAVACNRQKISEVKHISISHHDPDHVKGIRIVEKIGCDFITGENNPI
                     GFYALPEVIEDINRTNLDCLKYYGDVINCISINETSHVKINNFDIDLINNNPDRNITF
                     YVIKENEKKVIYACCNPKPFTHSDMYFDADILIISLVSDDGILNDGTKLKDTPFKDEI
                     FTLDEIVEIKNYYRIKKIIVTHIDEMWGKSYGYYSELEKKLDNIYFAYDGMEIIV"
     gene            complement(5596..6375)
                     /locus_tag="KPD58_002987"
     CDS             complement(5596..6375)
                     /locus_tag="KPD58_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HBG0266720.1"
                     /translation="MSYKKIAIITGANSGFGKEFLKLLINEEEITEIWAVARNKARLN
                     QLVDEFGSKVKIFSKDLSKIEEVKEIGTFLSKENVCIKYLINNAGFAKFCSYNDLSID
                     ESINMIDLNISAVVALGLICIPYMQKGSHIMNISSQASFQPLPYQNIYSSTKAFIRNY
                     TRALNVELKSKGINAIAVCPGWMSTNLFKRGIVVAEKGTKNFPGIVSPDVVAKKALKD
                     AKKNKDISVYGINTKSSHLLAKLLPQKMMMKVWLMQQHIKE"
BASE COUNT         2437 a          888 c          831 g         2334 t
ORIGIN      
        1 acatgcattt tttatattta atatagtaaa ataattaata actatatcca tgattggagg
       61 atttttatgg ttaaatccaa gaagaaaata tttgtttttc taattttaat tgcagttttt
      121 attatatttg catcatacat aagaaattat actaatgata caaaaaaaga ttctgttaga
      181 ggaactattc cttttgaaaa aatggaatat aagcgtccta atatacagtc aatttgcgaa
      241 aacattaaca aatgtaacga caaactttta acagctaaaa cagctaagga acaattaaat
      301 ttatttaatg aagttgataa aatttatcag gatttttaca gtacacttac tatagcaaaa
      361 attagaaata acatagattc atcagacgaa ttttactcta aggaatataa gtatcttatg
      421 agtaaatcag tagatgttga catgtcatac aaagactttc aagatagatt tgtaacttct
      481 aaattctcta aagaattaga aaaagaactt ggaaatgata catttaatta tctaaagaac
      541 attggaaaac ttaattctaa agaagttgaa ggtttgctta aaaaagagca agacctagtt
      601 gtaaaatatg aagacttatt agctaaatct acagtatcaa tagatggtat agaggtagac
      661 tttgaagaag ccttgtcaag accaaatctt agccctgaag aatatgtgaa aatttattca
      721 gattacttaa aaaaatacaa tcctatattt ggtaacatat tcttagaact tatacagaca
      781 agaactgaaa tagcaagtaa gcaaggattt aagaattata tagattatgc ctatatgaac
      841 ctaaacaaag actattctca agaagaagct aaaaaattta gacaagatgt aaaagattac
      901 atagttccat tatatagaga gatttcatct aaaccatctg attcttctat ttatattaaa
      961 gtatataaaa atcgctcatt taggaagttt gataaagtac ttgaagatat atctccaaaa
     1021 ctaaaagaga gcttcgacta tatgaaaaaa tatgacttgt acgattattc ttctggaaaa
     1081 aacaaatcac ctggaggata tactacttat ataaataaat ataaagcacc tttcttgttt
     1141 aatacatggg acaattcttt tttaggtgtt actagttttg ctcatgagtt tggtcatttt
     1201 tataattact acaacagtat taacttaaat aataagatgc aaccaagtat agatgtatgt
     1261 gaagttcatt caacatcttt agaaattcta ttttataaat attttgatga tttttttggt
     1321 aaacaatctg aagctataaa aaaagaacac ttatcgattg ttttaaatac tattattgat
     1381 gcttgtcttt atgatgaatt tcaagaaatt atatacaaga atccatatat gagtctaaat
     1441 gatatcaata aattgttttt tgatttagag gaagaatatg gagtttccaa taatatactt
     1501 agagaaaaaa atgctccttt ttggatactt gtatctcata acttccaagt tcctttttat
     1561 tacttgagtt atggtcttgc atctgatgta tcacttcaga tatggcaatt atcacaagaa
     1621 gattacagaa aagctgttga tgtatatatg gattttttaa atcaaaatac tgatgctgga
     1681 tttaaagatg tggtagaaaa ggtaaattta caattacctt tccaaaatgg aaatttagag
     1741 gaaataagct ctactttata tgattatttt ggtatagata atccacttga actaaaaaat
     1801 gcttcttaat ttctaatact ttatactcac tcttaagatt tagtattaga ttgaaataag
     1861 tgatattttt tcacacacaa ttaatgttta ataaaattat atggtaaagt atcagaaatt
     1921 tttaagactc ttgtctttat aatttctgat actttattta ttaataatat tattattttt
     1981 ttatatatat aattaaaagg attgtggaaa tcaataaaaa tatccctgta aaaatagcac
     2041 tattcatatg gaacttaata gaacttgcat ctgttacctt attattctta atccagaacc
     2101 cacaaaccat agttgaaaga aaaagtaaaa cagtgataag acttgaacca atagttataa
     2161 ttttcatctt acatcaacat cctctccaat aatattttct attaaaatat caatgaattt
     2221 atctcgctca tatttattct ttatatttat accactgtat aactgaaatg attctgtaga
     2281 taaagcagaa atctgtattg gtaaaataat ttgcagggca atgattctta gactcatttc
     2341 atctttttta tttccaaaaa ttttacgaag catagggaca atatcaagtt ctgcttgcat
     2401 atctccattg gttatacatt gattcaatac aaaaggaagc actttttcat aatgtaatcc
     2461 agtatcacac aattttttta ataaattttt taaatcttca ataggtctta gagtatatac
     2521 actatcgtca tatagctctg ctattatatt tgacatcaca tctcctatgg cagtactcaa
     2581 caaattatcc tttgttttaa aatgataatt tattaaacca actccaacat ttgcacgctc
     2641 agcaattttt ctaactgtaa tctcctcaat atcatctact tcatttaaaa gattcaatgt
     2701 aacttcaatt atacgttctc tagcatcttt atcattactt ttcataatag acctccaaaa
     2761 caattaacat tttcagttct gaacatgttc agtataatat ttttttcatc atatgtcaat
     2821 ataatttaat tataatttta gttgactcta acattatgat atggtttata ttaatattaa
     2881 gctaaaagta cctttataaa ggagacaaaa acatgtttaa gataggcgat ttttcaaagc
     2941 taagtaaaat tagtattcgt atgttacgtc attatgatga aataggactt ttaactcctt
     3001 ctcatacgaa taaaactaat ggatatagat actatagtgc agaccaactt tctactacaa
     3061 acagaatcca tgcactaaaa gatatgggct ttggcttgta ttctataaaa gaaattttga
     3121 cagaatataa cgataaagaa agtctcataa aatatctaaa tatccaccat tctcaagtaa
     3181 aagaacaatt agaagataca cagaaaaaac tattaaaaat agaaaccact attaaaagaa
     3241 ttggaggaaa tgatattatg aaaaattatg atgtaactat aaagaatttt gcaccaaaat
     3301 atatgatgac acttagaagg gttataccaa cttatcaaga cgaaggaatg ttatggcatc
     3361 aagcattttt agagacaaaa gaccagaatg ttcaaattga acccccaaag tattctaaaa
     3421 cagtttttta tgatactgga tataaagaag attatgtaga tgttgaagta caagttgctg
     3481 tatctggaaa gtataaagat actgaacatg ttaaatttaa gactgttcca agtgtgactg
     3541 ctgcaactgc aattgttaat ggaaatttca atcaagttgc agacgcttgt gaggctattg
     3601 gtaattggat atcagataat aactatgatg tggatggtcc tatgtttaat atttatcatg
     3661 taagtcctgg caacgatagt aatcctgata attgggtaac agaggtatgc ttcccagtta
     3721 aaaagaagta atttttatat taattggata atattttaat ttgttagaca aattactttt
     3781 agtgttatgt ataaatacct acaatttgat taggatatta tcttaatttg ttaaacaaat
     3841 tacttttagt gttatgtata aatatctaca atttaattag gatattatct taatttgtca
     3901 gataaatcac ttgtgatgtt atgtttaaat gactagaatt tgattgggat attatcttaa
     3961 tttgtcagat aaattactta taagattaca tacaaccctc atactctagt taaatatttg
     4021 aatcactaat ataaattagt ttttaacaaa acaaagagta aagtacaaat aattcctttg
     4081 tactctactc tttttaactt tctttgtaca ataatacaat gataaatata aaaaattata
     4141 tattccattc tatagaaata tgctcaccaa taacatgtat gttgttaatc aatatattat
     4201 atcaaattat atcaaaatta cataaacctt ataggacaat catatcttat tacaagaaaa
     4261 atgtcttatc catattgaat aagtcattcg gttcatatac aaagtcaatt tcattgagaa
     4321 atatttaata tatgcttata aaaatttcat actattcttt catatttaca tgtgatataa
     4381 tttattaact cataattttg ttaatcagtt gtgattgtag attatttata taatctaata
     4441 tttttagaag gtggtgtaat aactttgaaa ttaaaaattg ttggtagtgg cggtatgtca
     4501 attattccaa gttcattttg caaatgcaaa atatgtgaag aagccagaca aaaaggcgga
     4561 agatatgaac gattaggacc aagtttatat attgatgata taaaaatgct gatagacaca
     4621 cctgaagaca ttgcagtagc ttgtaacaga caaaaaatat cggaagtaaa acatatttca
     4681 atttctcacc atgacccaga ccatgttaag ggtattagaa ttgtcgaaaa aataggatgt
     4741 gactttatca caggagaaaa taatccaatt ggcttttatg cgcttcctga agtaattgaa
     4801 gatataaacc gaacgaatct cgattgtctt aaatattatg gtgatgttat aaattgcatt
     4861 tctattaatg aaacttcgca tgtaaagata aataattttg atattgattt gattaacaat
     4921 aacccagaca ggaatataac cttctatgtt atcaaagaga atgaaaaaaa agtcatatat
     4981 gcctgctgta atcccaagcc atttactcat agtgatatgt actttgatgc tgatatttta
     5041 ataataagtc tagtttctga tgatggtata ttaaatgatg gtacaaaact taaagacaca
     5101 ccatttaaag atgaaatatt tactttagat gagattgttg aaattaaaaa ctattataga
     5161 attaaaaaaa ttattgttac acatattgac gaaatgtggg gaaaatcata tggttattac
     5221 agtgaattag aaaagaaact tgataacatt tattttgcat atgatggtat ggaaattata
     5281 gtctaaatca ttaagtaata agcataatgc aaaataagaa aacttcaatt taacagtata
     5341 gtcaaactat gtgatatcaa catatcgcag tttttacaaa atatcatagt atttatcttt
     5401 ataaaatttc aagtcctaca tattttaata cttcaatatc taaataaagt aaaatcaaaa
     5461 atatatttta tatacattat agacggctta tcgtcactag ataagccgtt ttgtcacaat
     5521 tattatttgc tcaacaaaac ttctcataat atataacaga ttgaatctcc aactgatact
     5581 taattaccaa aactcttatt ccttaatatg ttgttgcatt aaccaaacct tcatcatcat
     5641 tttttgtggc aatagttttg ctaataaatg actggatttt gtatttatac catacacaga
     5701 aatatcctta tttttctttg catccttcaa tgcttttttg gcaacaacgt ctggagacac
     5761 tatgcctggg aaattttttg tccctttttc agctacaact atacctctct taaatagatt
     5821 tgtactcatc caacctggac atacagcaat tgcatttatc cctttactct taagttcaac
     5881 atttaatgct cttgtataat ttcttataaa cgctttagtt gaactataaa tattttgata
     5941 tggcaatggc tggaatgatg cttgtgaaga aatattcatg atatggcttc ccttttgcat
     6001 atatggaata catataagtc ccaatgctac aacagcacta atatttaaat caatcatatt
     6061 aatagattca tctatactta aatcattgta tgaacagaat tttgcaaaac cagcattgtt
     6121 tataaggtat tttatacata cattttcttt acttaaaaat gtaccaattt cttttacttc
     6181 ttctattttt gataaatctt ttgaaaaaat ttttacctta cttccaaatt catctactaa
     6241 ttgatttaat ctcgccttgt ttcttgcaac tgcccatatt tctgttattt cttcttcatt
     6301 tattaataac tttagaaact ctttaccaaa accactatta gcacccgtta taattgcaat
     6361 cttcttataa ctcataatta ttctccttta ccataagttt atacgctcaa actgtaaact
     6421 tattttttac aaataaaaca ccctctattc agatgttata ttcatattat ttgagatatt
     6481 atatttatat
//
LOCUS       DAELLV010000199         6460 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.199, whole genome shotgun sequence.
ACCESSION   DAELLV010000199 DAELLV010000000
VERSION     DAELLV010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6460
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.199"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            194..1597
                     /locus_tag="KPD58_002988"
     CDS             194..1597
                     /locus_tag="KPD58_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG0266721.1"
                     /translation="MEKKKIGLWSLVFMNVSALYGIRWIAKSTASSFGLGLGAIPMWL
                     LFSFIYFVPGALICAELASTYQKGDGGLYDWVKEAFGEKYGFLVSWLNWTAKIFWYSS
                     FLTFLAVNVSYTIGNPALADNKMFVLILSLIIFWALSLIATKGISFTKIFTNTGALGS
                     TIPSAILIIFSFVAVFVLKKHDIASTYTIASMTPKLNMDAFVAISAIMFGLAGAETAA
                     NFITEIDKPEKNFPKAILISAGIVASLYVLGSIAITMIIPPDQITASKGVLDALSAVC
                     ASLGIGSWLVQLIAFGIAFSVLGAIVLYIASPIKMLFGSVKKGIFPDSLVEVNEHKIP
                     SKAVILQAIIVTIILLVTTLMPSVDAIYNVLVTMTALTALFPYVLLYASYIKLRKERP
                     DEIRPYTMAKSTSTCLGLAKMVLVVTVVGILLSAAPVMPTFAENVIYEIEMIGGGLLV
                     ILSGLWLWNRYEKKTAK"
     misc_binding    1688..1928
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1971..2669
                     /locus_tag="KPD58_002989"
     CDS             1971..2669
                     /locus_tag="KPD58_002989"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBG0266722.1"
                     /translation="MNVIGIIGAMDEEVSILVDLMDIRETIKKASLEFYKGILEGKNV
                     VLVKCGIGKVNSALCAQILISEFKVDAIVNTGVAGALNEKLDVNDIVISTDAIQYDVD
                     TTAFGDPKGVIPRMKTSVFKADERLIDAAYKSSVEEVKTHKVLKGRVVTGDKFINSKE
                     LKEELVNDFGGYCGEMEGGAIAHVCYLNNTPFVIIRAMSDKADGSADVTYDVFVHDAA
                     NNSKDIVLNMLKSI"
     gene            2692..3693
                     /gene="trpS"
                     /locus_tag="KPD58_002990"
     CDS             2692..3693
                     /gene="trpS"
                     /locus_tag="KPD58_002990"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBG0266723.1"
                     /translation="MSEKKVIFSGAQPSGKMTLGNYLGAIKNWTELQDNYDCYYSIVD
                     LHAITVPQDPKVLRANTIELLAQYIACGLDPEKNTIFIQSHVKEHVELMWVLNTMTYM
                     GELSRMTQFKDKSQKSEANLNAGLFTYPVLMAADILLYQTDLVPVGDDQKQHLELARD
                     LANRFNNRFSPTFVVPEGYYPKGGARVMSLQEPTKKMSKSDSNENAFILLADDSDSIK
                     RKIKRSVTDSLGVVKYNDEQPGIKNLIDIYSNLSKKSVEEIVNMYEGKGYGIFKEDVA
                     EVVSEALRPIREKYVDLLNNKDYLEKIYSMGAEKAEKQARKTLRKVYKKVGFIERRY"
     gene            complement(4345..5912)
                     /locus_tag="KPD58_002991"
                     /pseudo
     CDS             complement(4345..5912)
                     /locus_tag="KPD58_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021426623.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iota toxin protein Ib"
     gene            complement(5887..6030)
                     /locus_tag="KPD58_002992"
     CDS             complement(5887..6030)
                     /locus_tag="KPD58_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266724.1"
                     /translation="MYVYYFESPEKFAFNKVIRTKNQNEISLEKFNEFKETIQDKLLP
                     YNR"
     gene            complement(6248..6430)
                     /locus_tag="KPD58_002993"
     CDS             complement(6248..6430)
                     /locus_tag="KPD58_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266725.1"
                     /translation="MKKFRKHKSISNCISILLILYLTLGSLLPNNIYAQDLQSYSGKV
                     CNTTYKAPIERSEGFF"
BASE COUNT         2288 a          772 c          998 g         2402 t
ORIGIN      
        1 aattaaagtg gtaaaacttt taaatttatc atttttaata agatttgatt tcaaagtatt
       61 taaggtttat tattaaaact gtttttgaat atgcaaatat tttgaaaaat atcagtttaa
      121 taataatgaa tctattttat taattttgaa attaaattta aaataattaa tagagagggg
      181 aaattaaatt aggatggaaa agaaaaaaat aggcctatgg agtttggtat ttatgaatgt
      241 atcagcttta tatggtataa gatggatagc taaatctaca gcatcaagct ttggtttagg
      301 attaggtgca atacctatgt ggttattatt ctcatttata tactttgtac caggagcttt
      361 aatttgtgct gaattagctt ctacatatca aaaaggtgat ggaggtcttt acgattgggt
      421 taaggaagcc tttggagaaa agtatggatt tttagtttct tggttaaact ggacagctaa
      481 aatattttgg tattcttcat ttttaacttt cttagcagta aatgtttctt atacaatagg
      541 aaatccagcc cttgctgata ataagatgtt tgtacttata ttatctttaa taatattctg
      601 ggcattatct cttatagcaa ctaaaggaat aagttttaca aaaatattta ctaatacagg
      661 tgcattaggt tcaacaatac caagtgctat acttataata ttttcatttg tagcagtatt
      721 tgtgttgaaa aaacatgata tagcttctac atacacaata gcttcaatga ctccaaaact
      781 taatatggat gcatttgttg ctatatcagc tataatgttt ggtttagcag gagcagagac
      841 tgctgctaac ttcataacag aaatagataa gcctgaaaag aacttcccta aggcaatatt
      901 aatatcagct ggaatagtag cttcattata tgtattaggc tcaattgcaa taacaatgat
      961 aataccacca gaccaaatta ctgcttctaa aggtgtatta gatgcacttt ctgctgtttg
     1021 tgcgtcttta ggaattggtt cttggttggt tcaattaata gcttttggta tagctttttc
     1081 agtattagga gcaatagttc tttacatagc atcaccaata aaaatgttat ttggtagtgt
     1141 taaaaaagga atatttccag atagtttagt agaggtaaat gaacacaaga taccttctaa
     1201 agcagttata cttcaagcaa taatagttac tataatacta ttggtgacta cattaatgcc
     1261 ttctgttgat gctatataca atgttcttgt aactatgaca gcattaactg cattattccc
     1321 atatgtattg ttatatgctt cttatattaa actaagaaaa gagagaccag atgaaattag
     1381 accttatact atggcaaaat caacaagtac ttgcctaggt ttagcaaaaa tggttttagt
     1441 tgtaactgta gtgggtatat tattatctgc agcaccagtt atgcctacat ttgctgaaaa
     1501 tgttatatat gagatagaga tgattggtgg aggtcttcta gttatattat ctggtctatg
     1561 gttatggaat agatatgaaa agaaaacagc taaataaaat gtattttaaa aatttctttt
     1621 ggatattata atatatagtt gacaatttat agtgtaagga atattataaa ataaagaaat
     1681 taataaatta aatcctgaga taagaaatag taaataaata tatttttgca cagagaatta
     1741 aggatgctga gaacttaata aaaagtattt attgaatggg tctttgagga gtaaaatgaa
     1801 atgtaattta atattacaga gtatttgata tcgtgtatgc acgttatagc atatattaag
     1861 atatatatat tcaattttga gtgtatataa atttaggtgg taccacggaa aatatatccg
     1921 tcctattgat ttaggacgga ttttttaata tagaaaattt aggaggaaaa atgaacgtaa
     1981 taggaattat aggtgcaatg gatgaagaag ttagtatttt agttgattta atggatatta
     2041 gagagactat aaaaaaagct agcttagaat tttataaggg aattttggaa ggtaagaatg
     2101 tagtgttagt aaagtgtggc ataggtaagg ttaactctgc tctttgtgcc cagatactaa
     2161 taagcgaatt taaggttgat gctattgtta atacaggtgt tgcaggagct ttaaacgaaa
     2221 aacttgatgt taatgatata gtaatatcaa ctgatgctat acagtatgat gtagacacta
     2281 cagcatttgg agaccctaag ggagttatac caagaatgaa gacttctgta tttaaagcag
     2341 atgaaagact tatagatgct gcttacaaat cttctgtgga agaagtaaag actcataaag
     2401 tattaaaagg aagagtagtt acaggagata aatttataaa ttctaaagaa ttaaaagaag
     2461 aattagtaaa tgattttggt ggatattgtg gagaaatgga aggtggagct atagctcatg
     2521 tatgctattt aaataatact ccatttgtaa taataagagc aatgtctgat aaggcagatg
     2581 gaagtgctga tgttacttat gatgtatttg tacatgatgc tgccaataac tctaaagaca
     2641 tagttttaaa tatgttaaaa tcaatataaa tatataagga ggtacaaaaa aatgagtgaa
     2701 aaaaaagtta tatttagtgg tgcacagcca tcaggcaaaa tgactttagg aaattattta
     2761 ggagctataa aaaactggac tgaacttcaa gacaattatg attgttatta tagtatagtt
     2821 gatttgcatg caataacagt acctcaagac cctaaagttt taagagccaa tactatagaa
     2881 ttacttgctc aatatatagc ttgtggatta gacccagaaa aaaatactat atttatacaa
     2941 tcacatgtca aagagcatgt tgaacttatg tgggtattga atactatgac ttatatgggt
     3001 gaattaagta gaatgactca attcaaagat aaatcacaaa agagtgaagc aaatctaaat
     3061 gcaggtttat tcacatatcc agttcttatg gctgctgaca tattgttgta tcaaactgac
     3121 ctagttccag ttggagatga ccaaaaacaa catttagaat tagctcgtga tttagcaaat
     3181 agatttaata atagattttc acctactttt gtagttccag aaggatacta cccaaaaggt
     3241 ggagcaagag tgatgagttt gcaagagcca actaaaaaaa tgagtaaatc cgattcaaat
     3301 gaaaatgcat ttatactatt agcagatgat tcagattcta taaagagaaa gataaaaaga
     3361 agtgttactg attctttagg tgttgtaaaa tacaatgatg aacaaccagg tattaaaaat
     3421 ctaattgata tatattctaa tttaagtaaa aagagtgtag aagagattgt aaacatgtat
     3481 gaaggaaagg gttatggcat atttaaagaa gatgtggctg aagtagtatc agaagcttta
     3541 agacctataa gagaaaaata tgttgacctt cttaataata aagattattt agaaaaaata
     3601 tatagtatgg gagcagaaaa agccgaaaaa caagctagaa aaactcttag aaaagtttat
     3661 aaaaaagttg gatttataga gagaagatac taattattag ctatctttac ttgataaata
     3721 aattttaaag ttattaaaaa ggtatatgat atgaaagaga gagtctcaaa gtgaactttt
     3781 gttcatgagg cactcttttt tgtatgaaat aaaatatatt ttaatcattt caacaatgaa
     3841 aaagaggatt atctctattt tgagacagca tcttttgata ataaaaaaag atgagctttt
     3901 gctcatctca aaggggttag attattctta ctttggggaa agtagaataa aggtaaaagt
     3961 taactgatgt caactttgtc ttttttgcat atcactactg agcataaaaa aatcagaaaa
     4021 ccatagcctt ctgaaacaac ttctaacata cattgtattt tatacccaat atgtaattta
     4081 tgagttaatt ttaactctta taatgaaaat tgtcaataat atatcaaaaa aatatttttt
     4141 aaatttatat aattaaatga atttatgttt atatataggt gatatgatac agataattat
     4201 ctttttaata caatatagtt ctaaaaaaga atatattaga agaagataat atttaaataa
     4261 aaaatacctt ataaagttaa ttttttaata ctttataagg taaatacata ttgtattgtg
     4321 gggataaatt taaatcattt gatactaatc aacactaaga accaataact ctctatcatc
     4381 ttgagtaatt gtatatattc ttaatttctt gtatgtcata atattttctc cacctgtaaa
     4441 ataactccta agattaacac aatcagtttt aggttgattt gtatctccta aataattacg
     4501 caattcttta tctttagttt caatatcttt aaatccaaaa tattgtaaag tagcttcaac
     4561 tctatatttt tggacataat cgagagcttc tttattagtt tatgttggtg taaaatacat
     4621 accatttata taagccttta ttgtacttgg attaaattta aaatctgcag aatatatttt
     4681 atgtgtaact attatttctt gaacagttgg aattttaact tctggttcat caagtatttc
     4741 aggagtacta tttagctctg taatagataa gttatctaag tatattgtat cactatcaat
     4801 taatgctata tttatattag aagaatcttt ttcagtagtt tcaaattcat aactaaattt
     4861 tgtatatcct tgttctagta ccaaataatc cgttttttct tcttttactt ttatatttac
     4921 aattattgaa ttatatgtta aaggattttt tgaattttac taaaaacata acgtttataa
     4981 ggttttagtt cagacatagg tattttaatc ttcgtctcac catttaatct atttgctgag
     5041 ccttttaaac catcttgatt tgtagtatta acattactcc atatactagg gtaattattg
     5101 taatcatcaa aattagtaaa gtaagttggt gtttttataa gtatattcat tcctctttca
     5161 agtttaacat tacatatttg tttatcagac aaagttttta aactgtcttt aatcttattg
     5221 cctatattat catcaaatat gagttcaaca caactttcat ctattggtat attattaaaa
     5281 tataacaaac catctttttt agtagtgcca aaagtttttt caattgcttc tccaattgta
     5341 agttcaggtg ttttatcttc tgggacctgt aaacttttag cagtaattct tcttttgtaa
     5401 gattcatttt ctgtatctaa tataatagat acagaaatac tgtcaatttg acctatatcg
     5461 tcttaccaac tatttcattc tgttactatt tgtccagaac cattttttgt accaaaattt
     5521 ccacttactt gtgttgtttc taatttaatt tgctttccat catctaattt ttttaattaa
     5581 ttataattta taggaatcag tctagaacta aattgataca ttgtgttaag agccataggt
     5641 gaaaatcctt ttttgagata agtatttcca ggagatagat tattgccaat ttgattttct
     5701 tgtgctttga tagttgataa tgcatctcca tctaacacta aatttgttgt tggtgtcact
     5761 ttgtacatag gtgcagtacc tgtgttgtag tatctaatat ttgcatttat atatgcagat
     5821 tctcctttgt ttatacttaa tccagtattt catgattatc cattgctatt ttgaacagca
     5881 gttgaattat ctgttgtatg gtaataattt gtcttgtata gtttctttaa actcattaaa
     5941 tttttctaat gaaatttcgt tttgattttt tgttcttatt actttattaa atgcaaattt
     6001 ttctggagat tcaaaataat atacatacat aggtttatct attttatttt ttgatatagc
     6061 atttcgaagt tctctatatt ctttttctca agaatttgct tctatttgat aatcataaaa
     6121 ataatttctt gtctgagaat atttgcttat ctctatagaa tcttttttat atgatcctaa
     6181 tacttctttt tcagattttt caagtttttg atcttttctt tctcattctt tagccttttc
     6241 tttatcttta aaaaaatcct tctgatcttt ctataggagc cttgtaagta gtattacaaa
     6301 cttttccact atagctttgt aaatcttgtg cataaatgtt attaggtaac aaactaccta
     6361 aagttagata taatatcaac aatatagata tacaattact aatactttta tgtttcctaa
     6421 attttttcat ttattctccc tacaaatatt agtttagtta
//
LOCUS       DAELLV010000200         6456 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.200, whole genome shotgun sequence.
ACCESSION   DAELLV010000200 DAELLV010000000
VERSION     DAELLV010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6456
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.200"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            23..1738
                     /locus_tag="KPD58_002994"
     CDS             23..1738
                     /locus_tag="KPD58_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008721785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HBG0266726.1"
                     /translation="MDGYEEYTVLKEWEKTHKNEEIYSIRFGGNYMIDMKDGIKLSTD
                     VYLPDFVDSAKKAPTILMRTPYGKENDKEIYYKYVQRGYAVVIQDVRGRNESEGKWEP
                     MIHEREDGDSTINWIVSQEWSSGIVGMLGASYLGYVQWAAASSGNKHLKALVSIVTSG
                     SPFIDIPRKGGAFVSGMLAWAFMVSRNKVDRSKMVRDDWDDVLNIRPIENIPVEALGY
                     RIEFLEEWLKRVEKDEYWDLMDWHLQKDKINVPALVVSGWYDDNSMGTTEALDVIKDY
                     EKGKRKAILGPWMHNSNTLRDINGISLGNSSLRYDLDYNYLLWFDKYLKGIENNIDTT
                     APVEYYSVGSNKWKTEENWPIINKIDKSMYLISDGNANTSLGNGRLVFDNDLEEKYDS
                     YIYNPKDPSVQLIDMSENEVGVPNNYKDLEKRSDMLCYTSDAFSEEFTVTGDIKLEFF
                     ASSSAKDTDWVIKIMDVDLDGNSIKLADGILSARFRNSFYKSEFMEEGEIYKFTIITS
                     KISNTFKVGHKIRLDITSSAKNFIFQNSNTTEGYNSIEYIEAKNTIYHGGKYPSKLIL
                     PIENK"
     gene            complement(1897..2241)
                     /locus_tag="KPD58_002995"
     CDS             complement(1897..2241)
                     /locus_tag="KPD58_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBG0266727.1"
                     /translation="MEIKRYEGTGRMSRAVVHNNTVYLCGQTHAEGGVIEQTTEVLAK
                     IEDLLNKYGSDKQHLLSVTIYLRDMKDFEAMNSVWDAWVEKGFEPARACVEARLAREH
                     LLVEMSVVAATK"
     gene            2533..3423
                     /locus_tag="KPD58_002996"
     CDS             2533..3423
                     /locus_tag="KPD58_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBG0266728.1"
                     /translation="MSSTIKSNRIKGIMFIIASALGFAMMSAFVKLAGDLPSFQKVFF
                     RNLVSAIIAFWLIIKHKGSLTGKKENLKVLLYRSIFGTLGVIFNYYAIDKLMLSDANM
                     LNKMSPFLVVIFCAIALKEKINVKQIGAIIIAFIGALFIIKPTFSVEVIPYLGGIAGA
                     VFAAMAYTCLRVLGDREDYYTIVFFFSVFSLVTVGPIAFAVYEPMTLMQLIYLLLGGV
                     FASLGQFGITLAYKYAPAKEISIFDYSNIIFSAILSIFLFNVYPDMLSVVGYLIVFSA
                     AFYMFLYNKKLDKLDKEKKK"
     gene            3641..4927
                     /locus_tag="KPD58_002997"
     CDS             3641..4927
                     /locus_tag="KPD58_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBG0266729.1"
                     /translation="MEKVKVAVIGAGNRGTYAYAPYIYENSDVCEIVAVAEPKKGRRE
                     LFTQKYNLDSKNVFETLEDFFKHDKMADAVIIATNDDRHYDVAKLALEKGYHVLLEKP
                     MSNSLDGLVHIDDLCDKYKDKIFMICHVLRYSPFYNKLKEIVESKKLGELVSIQYNEN
                     IGYWHFAHSFTRGNWRNSNETSPLILAKSCHDMDILLYLVGSRCKKISAFGSLKHLNN
                     QNASGEMAQNCLQCLVEKKCPYSAKRIYLEKDRSINRAVHINPTEENLLNILKTSPYG
                     RCVYRCDNNVVDNMVNILQFENGVTATFNLCAFTKENGRTIKLMFSHGEVGGDLNKNE
                     IRIKEFGKNEEIVMNPSNQQNMVEEYDRNLIAEFIKLVSNKELEKGRVAAKEAIQSHV
                     MAFAAEYSRVSDEVVYIEEFFDSAKQMTKEIEETIF"
     gene            complement(5016..5459)
                     /locus_tag="KPD58_002998"
     CDS             complement(5016..5459)
                     /locus_tag="KPD58_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory YrvL family protein"
                     /protein_id="HBG0266730.1"
                     /translation="MIKISNKRRIPLGEFISVFLFFAVFFGITFCIFTVIGIGFLSFL
                     GFEYKSLGAVLIFFLIYFCITTPIDFLCTTILDIFRYVNKLPYSIYKLCEFIIDFILT
                     FLAMNIIDTFMDSVTIPLSTEILFALLSYLLSECMDFFDRDKKKP"
     gene            complement(5483..6277)
                     /locus_tag="KPD58_002999"
     CDS             complement(5483..6277)
                     /locus_tag="KPD58_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HBG0266731.1"
                     /translation="MKLYLSADIEGTCGIVNWDETKLECEFSQHYRTQMSKEITAACD
                     GATQSGVHEILVKDAHDSGRNINPNILPENVKILRGWTKDPLVMMAGIDESFDACIFT
                     GYHSGATCNGNPLSHTMDIDYDYFKINDEIASEFTINAYIAAYYNVPVAFLSGDEMLC
                     ESAKKLNPNIVTVPVSKGIGNGSISIHPNLALKKIEKGVKKALSGDLSRHLIKLPDKF
                     KIEIKFREHYKAFKASFYPNMKKIDSQTVLFETEDYYEFLRMLLFI"
BASE COUNT         2474 a          764 c         1066 g         2152 t
ORIGIN      
        1 agaaacttgt actatattaa ttatggatgg atatgaagag tatactgtat taaaagaatg
       61 ggaaaaaact cataaaaatg aagagatata ttctataaga tttggtggaa attatatgat
      121 tgatatgaaa gatggtataa aactttctac agatgtatat ctccctgatt ttgtagattc
      181 tgccaaaaaa gcaccgacaa ttcttatgag aactccatat ggaaaagaaa atgataaaga
      241 aatatattat aaatatgtac aaagaggata tgctgttgta atacaagatg ttaggggaag
      301 aaatgaatca gaaggtaagt gggaacctat gattcatgag agggaagatg gagatagtac
      361 cataaattgg atagtatcac aggaatggtc aagtggaata gttggaatgc ttggagcatc
      421 atatcttggt tatgtccaat gggcagctgc atcttcaggc aataagcatc ttaaagcttt
      481 agttagtata gtaacatcag gaagcccttt tattgatata ccaagaaaag gaggagcttt
      541 tgtatctggt atgcttgcat gggcatttat ggtatcaaga aataaagttg atagaagcaa
      601 aatggtaaga gatgattggg atgatgtttt aaacatacga ccaatagaaa atatacctgt
      661 tgaagctctt ggatatagaa ttgaatttct ggaagaatgg ttaaagagag tagaaaaaga
      721 tgagtattgg gatttgatgg actggcattt acaaaaagat aaaataaatg tacctgcatt
      781 ggttgtatct gggtggtatg atgataatag tatgggaact acagaggcct tagatgttat
      841 aaaggactat gaaaaaggaa agagaaaggc tattctaggt ccatggatgc ataattcaaa
      901 tactttaaga gatataaatg gtatttcttt aggaaatagt tcattaagat atgatttgga
      961 ttataattat ctattatggt ttgataaata cttaaaaggt atagaaaata acatagatac
     1021 gactgctcca gtagagtatt actctgtagg ttctaataag tggaagacag aagaaaattg
     1081 gccaataata aataagatag ataaaagtat gtatctaata agtgatggaa atgctaacac
     1141 atctttagga aatggtagat tagtatttga taatgattta gaagaaaagt atgatagtta
     1201 tatatataat ccaaaagacc catcagtaca actaattgat atgtcagaga atgaagttgg
     1261 agttccaaat aactacaaag atttagaaaa aagaagtgat atgttgtgtt atacatcaga
     1321 tgcttttagt gaagaattta ctgtaacagg tgatataaag ttagagtttt ttgcatcaag
     1381 ttctgccaaa gatactgact gggttataaa aattatggac gtggatttag atggaaattc
     1441 aattaaatta gctgatggta tacttagtgc tagatttaga aatagttttt ataagtcaga
     1501 gtttatggaa gaaggagaga tttataagtt tacgattata acttcaaaaa tatcaaacac
     1561 gtttaaagtt ggtcataaaa ttagactaga tataacgtct agtgctaaaa actttatttt
     1621 ccaaaatagc aacacgacag agggatataa tagtattgaa tatatagaag caaagaatac
     1681 gatttaccat ggaggtaaat atccatctaa attgatattg ccaatagaga ataagtaaat
     1741 ttatatagat taaatagtga ggaggtgtct caaaatgaat tttttagttc gtgagacact
     1801 ttcttttata cgaaatcaaa tatattttca tcatttcaac aatgaaaaag aggcttatct
     1861 ctctgttttg agatagcctc ctataagcta aattgattat tttgtagctg caacaacgct
     1921 catttcaact aacaaatgct ctctagcaag tctagcttca acacaagctc tagcaggttc
     1981 aaatcctttt tcaacccaag catcccaaac agagttcata gcttcaaagt ctttcatatc
     2041 tcttaaataa atagtaacag atagcaaatg ttgcttatct gaaccatatt tgtttaataa
     2101 atcttctatt ttagctaaaa cttcagttgt ttgttcaatt actcctcctt cagcatgagt
     2161 ttgtccacat aaataaactg tattgttatg aactacagct ctactcattc ttccagttcc
     2221 ttcatatctc tttatttcca tattttaaat acctccatag ttaaaaaatc tattaaaaat
     2281 ctaaataata aattaaaaaa agtctatact atataaaatt atattatata ttctgataat
     2341 aatccataaa aacatataac tttttagcta taattattat tgttattaat tgaaaagatt
     2401 tattagttac ttgacatagt ttacactttt aattatagta taaattggaa tatttagata
     2461 attattattt aatgtataag ctaagttttt aattaatatg gctttataca tttatataga
     2521 aaagaggtac gtatgagtag cacaatcaaa tcaaatcgaa taaaaggtat aatgtttata
     2581 atagcatcag cgcttggctt tgcaatgatg tctgcattcg taaagctagc tggagattta
     2641 ccaagttttc aaaaagtgtt ctttagaaat ctggtatcag ctataatagc tttttggctt
     2701 atcataaaac ataaaggaag tctaactggt aaaaaagaaa atttaaaagt acttttatat
     2761 aggtcaatat ttggaactct aggagttata tttaactact atgcaattga caagttgatg
     2821 ctttcagatg ctaatatgct aaataaaatg agtcccttcc ttgtagtaat attttgtgca
     2881 attgctctaa aagaaaaaat aaatgtaaag cagataggag ctattataat tgcatttata
     2941 ggagctttat tcataataaa accaacattt agtgtagaag taattccata tcttggagga
     3001 attgcaggag ctgtatttgc agcaatggca tatacttgtt taagagttct tggagataga
     3061 gaagattact atacaattgt atttttcttt tcagtttttt cattagttac tgtagggcca
     3121 attgcttttg cagtatatga accaatgacc cttatgcaac ttatatatct attactaggt
     3181 ggagtatttg caagtctagg tcaatttgga ataactctag catataaata tgcacctgct
     3241 aaggaaatct ctatatttga ctatagtaat ataatctttt cagcaatttt aagtatattc
     3301 ttattcaatg tctatccaga tatgttaagt gttgttggat atttaatagt attttcggca
     3361 gcattttata tgtttttata caataagaag ttagataaat tagacaaaga aaagaagaag
     3421 taaactagat aaaagtgttt ttataatttg tatttaatat ttaagtcttt gtattaaaat
     3481 taaagaaaat ttaattaaaa atgtttggta tattaactta taacagcagt taaaattgaa
     3541 aaattataac tgctgtttct tttattaaaa atatccaaat gtctgtaaaa atagactata
     3601 atagagtagt ataaataata taaaggggat aaattaaagt atggaaaaag tgaaagttgc
     3661 tgtgattgga gcaggtaata gaggaacgta tgcatatgct ccatatattt atgaaaattc
     3721 tgatgtatgt gaaatagttg ctgtagcaga acctaaaaaa ggaagacgag aactatttac
     3781 acaaaaatat aacctagata gtaaaaatgt atttgaaact ttagaagact tttttaaaca
     3841 tgacaagatg gcagatgcag taataatagc tacaaatgat gatagacatt atgatgtagc
     3901 aaaactagct cttgaaaaag gttatcatgt actattagaa aagccaatgt ctaatagttt
     3961 agatggtctg gtacatatag atgacttgtg tgataagtat aaagataaga tttttatgat
     4021 ttgccatgta ctgagatatt ctccatttta taataaatta aaagaaattg tagaaagcaa
     4081 aaaattagga gagctagtta gtatacaata taatgaaaat ataggatatt ggcattttgc
     4141 acatagcttt actagaggga attggagaaa tagcaatgaa accagccctt taattttagc
     4201 aaagagttgt catgacatgg atattttgtt atatttagta ggaagtagat gcaaaaaaat
     4261 atctgcattt ggaagcttaa agcatcttaa taaccaaaat gctagtggag agatggcaca
     4321 aaattgtctt cagtgtttag ttgaaaaaaa atgtccatat tcagcaaaga gaatttattt
     4381 agaaaaagat agaagtataa atagagctgt tcacataaat ccaactgaag agaatctatt
     4441 gaatatactt aagacaagtc catatggaag atgtgtttac agatgtgata ataatgttgt
     4501 agacaatatg gtaaatatac tacagtttga aaatggagtg acggctactt ttaatttatg
     4561 tgcatttact aaagaaaatg gaagaactat aaagttaatg tttagccatg gtgaagttgg
     4621 tggggattta aataaaaatg agataagaat taaagaattt ggaaaaaacg aagaaatagt
     4681 tatgaatcca tcaaatcaac agaatatggt agaggaatat gatagaaatc ttatagctga
     4741 atttattaaa ttagtttcaa ataaagaact agaaaagggt agagtagcag caaaagaagc
     4801 tatacaaagt catgtaatgg catttgcagc agaatattct agggtcagtg atgaagtagt
     4861 gtatatagaa gaattttttg atagtgctaa gcaaatgact aaggagatag aagaaactat
     4921 attttaatta ataaaaattt attaaataaa ataaagatag attaaataat agaaatacga
     4981 aaatatgtgt tctattaagg aatctatctt ttttattaag gttttttctt atccctatca
     5041 aaaaaatcca tacattctga taaaagataa gaaagtaaag cgaataagat ttctgtactt
     5101 aaaggaatcg ttactgaatc cataaaggta tcaattatgt tcatagctag aaaagttagt
     5161 ataaaatcaa ttataaattc gcaaagttta tatattgagt atggtaattt gtttacatat
     5221 ctgaatatat ctaaaatagt agtgcataga aaatcaattg gagtggttat acaaaaatat
     5281 attaaaaaga aaattaatac agctccaaga gatttatatt caaatcctaa gaatgataag
     5341 aagccaattc ctataaccgt gaaaatacaa aaagttatac caaaaaaaac agcaaaaaat
     5401 aaaaatacag aaataaattc gcctaaagga atccttcttt tattacttat cttaatcaac
     5461 tccttaattt atgatatgaa atttatataa ataataacat tcttaaaaat tcataatagt
     5521 cctctgtttc aaatagtact gtttgagaat ctattttttt catatttgga taaaaagagg
     5581 ccttaaaagc tttataatgt tctctaaatt ttatttctat cttaaattta tctggcaatt
     5641 ttattaagtg cctagataaa tctccagata aagctttttt gacacctttt tcaatttttt
     5701 ttagtgcaag gtttggatgg attgaaattg aaccatttcc aattcctttt gacacaggaa
     5761 cagtgactat atttggattt agtttttttg cactctcaca aagcatttca tctccactta
     5821 aaaatgctac aggaacatta taatatgcag caatataagc atttatagta aattcagatg
     5881 ctatttcatc atttatttta aaatagtcat aatcaatatc catagtatga gaaagtggat
     5941 taccattaca agtagctcca gagtgatagc cggtgaatat acatgcatca aagctttcat
     6001 caattcctgc catcataact agtgggtctt tggtccatcc tctaagtatt ttgacgtttt
     6061 caggtaaaat atttggatta atatttctac cactgtcatg tgcatcttta acaagtattt
     6121 catgtacacc agattgagta gctccatcac atgctgctgt tatctcttta ctcatttgcg
     6181 ttctatagtg ttgcgaaaat tcacattcaa gttttgtttc atcccaattt acaataccac
     6241 aagtaccttc tatatcagca cttaaataca atttcataat atataccccc ttatgtttat
     6301 tattgatgat aataacagat aacagaaaat acataatcaa taaagggcat aaaaatatta
     6361 tgtcttacta ttaactatta ttaaatctgt aactgaaaaa taactaaatt ggtataaaaa
     6421 aatataacac tatgtgtcat ttatttatat agtttt
//
LOCUS       DAELLV010000201         6452 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.201, whole genome shotgun sequence.
ACCESSION   DAELLV010000201 DAELLV010000000
VERSION     DAELLV010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6452
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.201"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(370..777)
                     /locus_tag="KPD58_003000"
     CDS             complement(370..777)
                     /locus_tag="KPD58_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266732.1"
                     /translation="MSKVAKATFYLMIVTIISKILGMGRELVLSSIYGTGLYTESYLT
                     AMNIPNIIFAAIGTAIVTTFIPMYQDISSKQGEKQALKFLNNVLNIIVGICIVVAILG
                     VIFSKQLVSIFAIGFEGERFLLTVKFTKILIKK"
     gene            complement(1251..2135)
                     /locus_tag="KPD58_003001"
     CDS             complement(1251..2135)
                     /locus_tag="KPD58_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG0266733.1"
                     /translation="MDTKKENNYENYICVGSSNVSNAMKMYLKEIEEYKMLSAGEEVE
                     LAKEIINSSSVAKEKFINSNYRLVVSIAKRYKRDSIDMLDLIQAGNIGLIKAVEKYDY
                     KKGYKFSTYATWWIKQSITRYIDDCENTIRIPIHLHQRINFVKKKKQELLNVLLREPT
                     IDEIADACGLEVDKVLELLRRDKNVVSLDTPLKEDEDSSLVEFIPSDADFKDVVIHEV
                     EQHNLKEKIEELLTGLGEQEQQVLRMRFGIDDDDPKTLEQIGKVFGVTRERIRQIEAK
                     AIRKLRHPSKLKQLKHFY"
     gene            complement(2426..2902)
                     /locus_tag="KPD58_003002"
     CDS             complement(2426..2902)
                     /locus_tag="KPD58_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HBG0266734.1"
                     /translation="MSKELVKDRVIKYLIEDLLVPQDMIDTNVELAEFEEGAEGILDI
                     VVNVKDEEDYYAPVMIVQCLDEDVELEGEVLQKQIEFLEEVDNITMSGRLVLTNGDAM
                     MYADWRGEEYDTEAALPTYDIMVKEFHEMEQQVKDLEEHHHHDENCGCGCNHHHEN"
     gene            3168..4343
                     /locus_tag="KPD58_003003"
     CDS             3168..4343
                     /locus_tag="KPD58_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HBG0266735.1"
                     /translation="MKFKKQISSIVVSTMLVLGSMNLSYADGTNVVTLGANLSESQKQ
                     QMLNYFGVKKDQVLVLDVTNAEERQYLQGVATEGQLGKVTISCSYVEPTKKGNGINVK
                     TANLTWVTSSMIASTLTTAGLEDANVIAAAPYPVSGTGALTGIMKAFEDASGKKLDET
                     KKEIASEELVVTGDLGDDIGQEKATGVMNDIKTEIVKNGTKDTNQIANTINNVVNNYN
                     ITITPEQKEQIKGVMKKIADQNYDYNSMKNTLDNVSDNVNEQLKKLGESVKGSGILDT
                     IGGWFSGIGDWFSGLFSGGDKKDLGILNNTNDESLGSDAVINSTEGVQINPQNNTNSN
                     NDSDTNSSKNDTSSNNENNDSSQTNENNTTNNSENQSNNNDSVGFFEKIKNWLSGLF"
     gene            complement(4424..5227)
                     /gene="proC"
                     /locus_tag="KPD58_003004"
     CDS             complement(4424..5227)
                     /gene="proC"
                     /locus_tag="KPD58_003004"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBG0266736.1"
                     /translation="MKKIGFIGAGNMASAMIGGIVNSKLVEPNMVIASAYSQGTLDRI
                     DANFGINTTKDSKEVTRTSDIVIVAVKPDIYDDILEEIKDFIDDNKIIVTIAAGKSIK
                     DIESIIGEDKKIVRTMPNTPALVNEAMSSLSINKNINKEDLEAVTEVFNSFGNTEVVP
                     EYLIEAVIGASGSAPAYVFLFIEAIADAAVIAGMPRPQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPGGTTIEAVKVLEEEGFRASVIKAICACIEKSKKMSE"
     gene            complement(5267..5851)
                     /locus_tag="KPD58_003005"
     CDS             complement(5267..5851)
                     /locus_tag="KPD58_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0266737.1"
                     /translation="MNKDIGAKIKQLRTQKQMTLKDMSEKTNLSIGFLSQLERGLTSV
                     ATDSLGKIASVLDVELTYFFMKPKEHKRAVLRSYEKEVFDVENSTFIHYHLSSSLKEK
                     TMLPRLIEILPSKSSEEICCYVHEGEEFVYVLEGTLTVFLGDEQIEMYPGDTIHYNSE
                     KNNHNWVNYTNKVVKILVVSIPNPFEKSDAVKEA"
BASE COUNT         2127 a         1097 c          724 g         2504 t
ORIGIN      
        1 aagcacagac tatatcttat ccttcagcat tatccgttaa ggtctaccca cttccactac
       61 caatagcttg tagtgtactt ccctcaagag gaatagtcgt tgaagtttct cctattcgga
      121 gtttacctgc tgattgccca ttttaaagta cttaggattt aaccttatac catctaacca
      181 attttttcta ctttcgtaac tttcacactt aggtttattt catccttatg ttttagtttg
      241 attagcttta ggggtttcca gcaattcaag tagtattgga taggttctat cctatctcta
      301 catataggtt tccctatatg ctgactattt tagctactaa ctcatgacta aagtcacgag
      361 tgtgcgtagc tattttttaa tcaatatttt agtaaatttt acggtaagta aaaacctctc
      421 tccttcaaat cctatagcaa atatacttac aagttgcttt gagaaaataa ctcctaatat
      481 agctacaact atgcatatac caactattat atttaaaaca ttgtttaaaa actttagtgc
      541 ttgcttttca ccttgtttac tacttatatc ttgatacatt ggaataaaag tagtaactat
      601 agcagtacct attgctgcaa atataatatt tggtatattc atagctgtta aataactctc
      661 tgtatataag cctgtaccat aaattgatga taatactaat tctctaccca ttccaagaat
      721 cttagagatt atagtaacta tcatcaaata aaaagttgct ttagctacct tgctcatttt
      781 atcactccac ttctaacata cctcttttta atcaagtaaa atttagctca ttagatataa
      841 ttctatcaac taatttgcct tgcttagccc tatcaaaata taattaacac ctatttctta
      901 atatatcaca aactacatta ttcttcatat ttttttattt ctccaataag ttttacattt
      961 ttcctatata aattgtatag cttcaattat attcaaagaa atggagttgc tacccgataa
     1021 ctttaaaagt aaaaaacaga ttttattatc tttttaaact atattctaac atattttgtc
     1081 catattgaga aattttacta aactattatg tactttatta aataaataaa ataacagcac
     1141 acctataaaa tacaagtgtg ctgttcataa acagatttta taaaaaaata attcctaagt
     1201 agagattaac atgctttctc atactctcat atataatgtg tctaaaaata ttaataaaaa
     1261 tgctttaatt gttttaactt acttggatgt cttaattttc ttattgcttt agcttctatc
     1321 tgtcttattc gttctctggt aactccaaat acctttccaa tctgttctag tgttttaggg
     1381 tcatcatcat caattccaaa tctcattctt aaaacttgct gttcttgctc tccaagacct
     1441 gtaagtagtt cttctatctt ttcttttaaa ttatgctgtt caacttcgtg tattacaaca
     1501 tctttaaaat cagcatctga tggtataaat tcaactaaac tactatcctc atcttctttt
     1561 aatggagtat ccaaagatac aacattttta tctcttctta ataattccaa cactttgtca
     1621 acttctaagc cacatgcatc tgcaatctca tctatagttg gctctcttaa taatacattt
     1681 aaaagttctt gtttcttttt ctttacaaaa tttattctct gatgaagatg tataggtatc
     1741 ctaatagtat tttcgcaatc gtcgatatat cttgttatac tttgttttat ccaccaagtt
     1801 gcataagtgc taaatttgta tcctttttta taatcgtact tttctacagc ttttataagt
     1861 cctatatttc ctgcttgaat taaatccaac atatcaatac tatctctttt atatctcttt
     1921 gctatgctca ctaccaacct ataatttgaa tttataaatt tttcttttgc tactgaagat
     1981 gagttgatta tttccttagc cagctctacc tcttctcctg ctgacaacat tttgtattcc
     2041 tctatttcct ttaaatacat tttcatcgca ttcgatacat ttgatgaacc cacacaaata
     2101 taattttcat aattattttc ttttttagta tccataatca aaccccttct attcatagaa
     2161 tttgctaaaa caaaaataaa taatttaaac ctacataatc tacaattaca taatatccca
     2221 ttctttaaat gaattgtttt gtatattatg tatacaaatt tataaaaact atacctataa
     2281 tattattcta cttctaacag caatatcctc cttatatata taatttattt tattatatgt
     2341 tgttatattc ttagtttttt gaatgatata tgtctaaaaa aagactgttt actaaacagt
     2401 ctttttttat atatatgctc aatatttaat tttcatgatg atgattacat ccacatccac
     2461 aattttcatc atggtgatga tgttcttcta aatcttttac ctgttgttcc atttcatgaa
     2521 actcttttac cattatatca taagttggaa gagctgcctc agtatcgtat tcttcccctc
     2581 tccaatcagc gtacatcata gcatctccat tagttaaaac caatctacct gacatagtta
     2641 tattatccac ttcttctaaa aattctattt gtttttgtaa aacttctcct tctaactcca
     2701 catcttcatc taaacattgc actatcatta caggtgcata ataatcttct tcatctttaa
     2761 catttacaac aatatctaaa attccttctg caccttcttc aaattctgcc aactcaacat
     2821 ttgtatcaat catatcttga ggtactaata aatcttctat taaatatttt attactctat
     2881 cttttactaa ttcttttgac atttattttt ctccttccga ttctataaca ttatcataat
     2941 catttctata ttatagtata ctacattaat tgttttttca atctcttaca aaaattattt
     3001 tttattatct tccttatatt ccctttttta ttacaaagtt gtaatcgcat tgatttaaaa
     3061 aatagtttat atttagtata atagaaatgt agcacatatt tttattcaat atattaataa
     3121 aaaaatatat tgaaaatgta atgtatgtta aatctaggag gaatattatg aagtttaaaa
     3181 aacaaattag ttctattgtt gtgtctacta tgcttgtact tgggagtatg aacctttctt
     3241 atgcagacgg tacaaatgta gttacacttg gagctaactt atcagagtct caaaagcagc
     3301 aaatgcttaa ttactttgga gtaaaaaaag accaagtctt agttttagat gtaacaaatg
     3361 cagaagaaag acagtattta caaggtgttg caacagaggg tcaacttgga aaagttacta
     3421 tctcttgctc atacgttgaa cctactaaaa aaggaaatgg aattaatgtt aaaacagcaa
     3481 acttaacttg ggtaactagt tcaatgattg cttcaaccct tacaacagct ggtcttgaag
     3541 atgcaaatgt tattgctgct gctccatatc ctgtaagtgg tactggagca ttgacaggaa
     3601 tcatgaaagc atttgaggat gcttcaggaa agaaacttga tgaaactaag aaagaaattg
     3661 cttctgaaga attggtagtt acaggtgact taggagatga tatagggcaa gaaaaagcaa
     3721 caggtgtaat gaatgatata aaaacagaaa ttgtaaagaa tggtacaaaa gataccaatc
     3781 aaatagcaaa tacaataaat aatgttgtaa ataattacaa tattactatt acaccagaac
     3841 aaaaagaaca aattaaaggt gtaatgaaaa aaatagctga ccaaaattac gattacaata
     3901 gcatgaaaaa tacactagat aatgtatcag ataatgtaaa tgaacaatta aagaaattag
     3961 gtgaaagtgt taagggttct ggtatactag atactattgg tggatggttt agtggaatag
     4021 gagactggtt ctctggttta ttctcaggcg gagataaaaa agatttggga atcttaaata
     4081 atactaatga tgagtctctt ggctctgatg ctgtaataaa ttctacagaa ggtgttcaaa
     4141 taaatccaca aaataatact aattctaaca atgatagtga tacaaatagc tcaaaaaatg
     4201 atacttcaag taataatgaa aataacgata gttctcaaac aaatgaaaac aacacaacaa
     4261 ataattcaga aaatcaatca aataataatg actcagttgg tttctttgaa aaaataaaaa
     4321 attggctttc aggcttattt tagaaattaa acaaataaaa agaattgcct aaaatggaaa
     4381 ttttctataa aaggcaattc tctttattta caataaattt ttattactca ctcatttttt
     4441 tagatttttc tatacatgca catattgctt ttatcacaga agctctaaaa ccttcttctt
     4501 caagtacttt aactgcttct atagtagttc caccaggtga acataccata tcttttaatt
     4561 caccaggatg ttttcctgtc tcaagtacca tttttgcaga acccataaca gcctgtgatg
     4621 caaacttata tgcttgtggt cttggcattc ctgcaattac tgctgcatct gctatagctt
     4681 ctataaataa aaatacatat gctggtgcag aaccacttgc accaattaca gcttctataa
     4741 gatactctgg tactacctca gtatttccaa aagaattaaa tacttctgta actgcttcta
     4801 agtcttcttt gtttatattt ttatttatcg aaagagaaga catggcttca tttacaagag
     4861 ctggagtatt tggcatagtt ctaactattt ttttatcttc tcctataata gactctatat
     4921 cttttataga ttttcctgct gctattgtaa ctataatttt attgtcatct ataaagtctt
     4981 ttatctcttc caaaatatca tcatatatat ctggtttaac agctacaatt actatatcag
     5041 aggttctagt tacttccttc gagtcttttg tagtatttat tccaaaattt gcatctattc
     5101 tatcgagagt tccttgacta tatgctgatg ctataaccat attaggttca actaattttg
     5161 aatttactat tccccctatc atagcgcttg ccatatttcc tgcacctatg aatccaattt
     5221 ttttcacttt agtacctcct tattgactaa tagtaatcgt taaaatttaa gcttctttaa
     5281 ctgcatcaga tttttcaaat ggatttggta tacttactac caaaattttc actaccttat
     5341 tagtatagtt gacccaatta tgattgttct tttcactatt atagtgtatt gtgtctcctg
     5401 gatacatttc tatttgttca tctcccaaaa acactgtaag agtaccctct aatacataca
     5461 caaattcctc accttcatgt acgtaacaac aaatttcctc actactctta cttggaagaa
     5521 tttcaattaa tcttggtagc attgtttttt cctttaaact agatgataaa tgataatgta
     5581 taaatgttga gttttctaca tcaaatactt ctttttcata acttcttaat acagctcttt
     5641 tatgctcttt tggcttcata aaaaagtaag ttaattctac atctagtaca cttgctattt
     5701 ttcctaagga atctgttgcc acacttgtta atcctctttc aagttgtgat aaaaaaccaa
     5761 ttgacaagtt tgttttttca ctcatatcct tcagcgtcat ttgcttttga gttctgagtt
     5821 gctttatttt tgctcctatg tccttattca aaaccatcac ccctaaataa ttttaatttt
     5881 ttcataatat aaatacaaaa atataaatat aggtactgaa cccatcttta tttcatttaa
     5941 aaaaattttt ataagtatta ctataattta tcaatctttt tttgtctaca tttttacgat
     6001 tatttacgat ttatttttca catatgtata tttaatcttg ccaactaaag atacattttt
     6061 tataagcatt tactctttat ataataaaac ttaagttgtt catgtagtct tcttctggtt
     6121 taattattaa aatttcaaat tatattatta tttctacaca ttattacatc ctttagtaca
     6181 attataacat aattttacaa tttaatgata tctattcaat gtcttttttt actctacaac
     6241 atattttatt ttagagttta atactatata tattttttat atacagcctt gtcatttttt
     6301 agattgtcaa gagttattga agtattttat gtagatttag attttatttc ctaatttctc
     6361 atttaacaag aattgcttat gataaaaaat aatatcatct caataagatt cttgtaaata
     6421 tatctatatt ctaaatataa tttctttttt tg
//
LOCUS       DAELLV010000202         6440 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.202, whole genome shotgun sequence.
ACCESSION   DAELLV010000202 DAELLV010000000
VERSION     DAELLV010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6440
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.202"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            104..1429
                     /gene="cdeA"
                     /locus_tag="KPD58_003006"
     CDS             104..1429
                     /gene="cdeA"
                     /locus_tag="KPD58_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter CdeA"
                     /protein_id="HBG0266738.1"
                     /translation="MENLFTRKFTTFEFLKFVSPAIISMIFISLYTIIDGIFVSTLVG
                     SDALASINIVLPIINLVCGFGIMMATGGGAIVSIRMGENRQDEANSTFSFIVLFSLIV
                     GILFTVISYFFIKEISILLGATDKLLPYCITYGKVMILCTPFYILKFIFEYFARTDGN
                     SKFSLFLSVIGGVTNIILDYVFIKYFGMGLLGAAVATAIGIILTCVLGIIYFLSNKST
                     LKLRKPKTDFRLIRDTMINGSSEMVTELSTGITTFLFNVVALKLAGENGLAALTIVLY
                     AHFLMTSVYLGFAAGVSPLISYNFGAENSDKLKETFKHSLKFIFVSSLLVFIIALVFA
                     PFIVRVFVSPDNTVFKLALQGLKIFAFAFLFVGINIFASGFFTAFHNGKISAIISFSR
                     AFVFIIIGIIILPPMLNMTGLWLTVPFAEVITIFISILFIKKYKCRYKY"
     gene            complement(1524..2318)
                     /locus_tag="KPD58_003007"
     CDS             complement(1524..2318)
                     /locus_tag="KPD58_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0266739.1"
                     /translation="MNELKPYIKFFNKNTKLYIIIFMIVGIVMNILPVFILKIFGEAS
                     KTNLSTIVNPILTYITILLFVYGVNIANKDFSGALSIRADRKSCIKAIVIDLIILSFV
                     ISILGIVLIFLSKLFIEIITGYSIELSVLLKRDMIWTSISALIDPSTSSMPLTYLNYF
                     VQICINEICIGFIGVLLGAFTYRVRKVTSITILIGLPILMIIYIVNFATKNMHKMLLY
                     LEKIIYSFQNPFILFGTKVGIIVICIIFIILLLRKAPIKEYANDLL"
     gene            complement(2319..3194)
                     /locus_tag="KPD58_003008"
     CDS             complement(2319..3194)
                     /locus_tag="KPD58_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0266740.1"
                     /translation="MSSSIKIENLSHSFKKQNIFNNMSLNFEKNKIYGLLGKNGAGKT
                     TLLNIMVNQLIQNEGEIEILGKNPKEDIKVLEEVCIVREKEFYSPDFKVGQIFEFSSS
                     FYKSYDKGLEEELCKYFDLNTKKKYKQLSRGMKTILSNIIGICSNSAITIFDEPTIGL
                     DAVNRQEFYNIILDSYIKNPRTIIISTHLIDEIDDLLEHVIILNEGKIIIDEEIDVIK
                     QKAHYITGNKEELEKLECIKNIKPKKAFGNAVAYFYYGDFSENDEKILKNSNIDVGYI
                     GLQDMFVNMTKKEEL"
     gene            complement(3198..3566)
                     /locus_tag="KPD58_003009"
     CDS             complement(3198..3566)
                     /locus_tag="KPD58_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0266741.1"
                     /translation="MKLVLNNEEPIFIQIARAIEDEILSNGIKEEEQVPSTTELSKLY
                     KINPATVLKGINILVDKNILYKKRGIGMFVSDGAKTIIKEARKENFKHNFVKNLLQEA
                     NKLEINREELVDIIINFKED"
     gene            complement(3749..4408)
                     /gene="deoC"
                     /locus_tag="KPD58_003010"
     CDS             complement(3749..4408)
                     /gene="deoC"
                     /locus_tag="KPD58_003010"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBG0266742.1"
                     /translation="MKHILKTVDHTILKATTTWEDIKILCDEAVDMSVASVCIPPSYV
                     KRASEYLKGKIKICTVIGFPLGYQTTATKVFEAKDAIENGADEVDMVVNISDIKNKDY
                     DNIGKEIKEIKKAIGDKVLKVIIETCYLDEDEKIKMCEIVTMSGSDFIKTSTGMGTGG
                     ATLEDIKLMKEHVGKNVKIKAAGGVKSISDAEKFIEAGAERLGTSSICKILKNEDTTD
                     Y"
     gene            complement(4444..5631)
                     /gene="murJ"
                     /locus_tag="KPD58_003011"
     CDS             complement(4444..5631)
                     /gene="murJ"
                     /locus_tag="KPD58_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBG0266743.1"
                     /translation="MKQEPCDFSHGRFRTGIIFIGITSVMSAFLQIKENFIVVGFGSI
                     PYNIVIIISIMLSTVFGPYILPIGAVVAMVVQLLFYMFFVKKTNYKYLYYLNFKDDSL
                     IKLLALLSPVFIGVAVNQVNSLVDTTLASTLVKGSIPALTYADRLNGFVTGTFTASIV
                     SVMYPMLSKLSAENNQKKFTSSVKSSINMIIISMIPISVASIFFATPVVRIIFERGAF
                     DARATQMTATALIFYAVGMTAFGLRDILGKVFYSLQDTKTPMVNGIISVGVNIVLDLV
                     LIKPMAHGGLALATSSSSIACILLLFLNLKRKVGYFGQDKIIKATLKSVVASLIMGVL
                     SYFTYKFIFGILGVGTFNEFVSLAISVIVGGGIYTLLMTIFKVEEVDMILNIAKRKLH
                     LKK"
     gene            complement(5708..>6440)
                     /locus_tag="KPD58_003012"
     CDS             complement(5708..>6440)
                     /locus_tag="KPD58_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG0266744.1"
                     /translation="LPKLGMVKIRDKQVPKGRILNATISKEPSGRYYVSLCCIDVDIE
                     VFENTNNHIGLDLGIKEFCISSCGEFIENPKYLKKSLNKLAKLQRELSRKTIGSLNRN
                     KARLKVARLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNMIRNRKLSRLISDV
                     SWSEFIRQLEYKANWYGRQIVKVGKFFASSQICNKCGYKNEEVKNLNIREWICPSCNE
                     THDRDINASINILKEGLRLITIQNK"
BASE COUNT         2118 a         1022 c          713 g         2587 t
ORIGIN      
        1 ttgttgattt taagctgttt atcgtataaa taaaattttt cttgactata tagttactat
       61 atagcttatt atatatagag agtaataaaa ggaggtaaaa tttatggaaa atttatttac
      121 aagaaaattc actacttttg aatttctaaa atttgtttct cctgcaatta tatccatgat
      181 atttatatct ttgtatacaa taatagatgg catctttgta tcgacattag ttggttctga
      241 tgctcttgct agtataaata ttgtactacc tataattaac cttgtttgtg gatttggaat
      301 aatgatggca actggtggag gtgctatcgt ttctatacgt atgggtgaaa atagacagga
      361 tgaagccaac tctacatttt cttttatagt tttgttttca ttgattgttg ggattttatt
      421 cacagtaatc tcatatttct ttatcaaaga aatatctatt ttgcttggtg caacagataa
      481 gttattacca tattgtataa cttatggtaa agttatgatt ttatgtacac cattttatat
      541 tttaaaattt atatttgagt actttgcaag aactgatgga aattctaaat ttagtttatt
      601 tctatcagtc attggtggtg taacaaatat aatattggat tatgtattta ttaaatattt
      661 tggaatgggt cttttaggag ctgcagttgc aactgctata ggtattattt taacttgtgt
      721 tttaggtatt atttacttct tatctaataa atctacacta aaattaagaa aaccaaaaac
      781 cgattttaga cttataagag atactatgat aaacggttct tctgaaatgg ttacagaatt
      841 atctacagga attacaacat tcttatttaa tgtagtagct ttaaaattag caggagaaaa
      901 tggacttgct gctcttacta tagtattgta tgctcatttt ttaatgacat cagtctatct
      961 aggattcgct gctggagtgt ctccattaat aagctataat tttggtgctg aaaacagtga
     1021 taaattaaaa gaaacattta aacattctct aaaatttata tttgtttctt ctcttttagt
     1081 gtttattatt gctttagtat ttgcaccatt tattgttagg gtctttgtaa gtccagataa
     1141 cacagtattt aaactagcct tacaaggatt aaaaatattt gcatttgctt ttttgtttgt
     1201 tggtataaat atatttgcat caggattttt tacagcattt cacaatggaa aaatttcagc
     1261 tattatatct tttagtcgtg cctttgtttt tataatcata ggaatcataa ttcttcctcc
     1321 tatgttaaac atgactggat tatggcttac agttccattt gctgaggtta taaccatatt
     1381 tatatctatt ctatttataa aaaaatataa atgtagatat aagtattaaa aattaatact
     1441 taaaatcaac atttattaaa actaataaac ctgctttaaa atacaaattt acaaatttga
     1501 tttttaagca ggttttattt tagttataat aagtcatttg catactcttt aattggtgct
     1561 tttctaagta ataaaattat aaatattatg caaataacta taattcctac ctttgttcca
     1621 aataaaataa aaggattctg aaatgaataa ataatttttt ctaaatacaa tagcatttta
     1681 tgcatatttt tagttgcaaa attaactata taaattatca ttaatatcgg taatcctatc
     1741 aatatagtaa tactagtaac ttttctaact ctatatgtaa aagctccaag taacactcct
     1801 ataaaaccta tacatatttc atttatacat atttgtacaa aataatttaa ataagttagt
     1861 ggcatacttg atgttgaagg atctatcagt gctgatatag atgtccaaat catatctctc
     1921 tttagtaaaa ctgatagttc tatactgtaa cctgttataa tttctataaa taacttcgat
     1981 aaaaaaatca aaactatacc taaaatagaa atcacaaagc ttaaaataat taaatctatg
     2041 acaatagctt ttatacaact ttttctatct gctcttatac ttaaagctcc tgaaaagtct
     2101 ttatttgcta tatttactcc atacacaaat agcagtatag ttatatatgt taaaatagga
     2161 ttcacaatcg tggataaatt agttttagat gcttctccaa aaatcttgag aataaacact
     2221 ggcaaaatat tcataacaat tcccacaatc ataaatataa ttatatacaa tttagtattt
     2281 ttattaaaaa attttatata tggctttaat tcattcaatt acaattcctc cttcttagtc
     2341 atgttaacaa acatatcttg tagccctatg tatcctacat caatatttga atttttaagt
     2401 attttttcat cattttcact aaaatcacca tagtaaaaat aagcgactgc atttccaaat
     2461 gcctttttag gttttatatt ttttatacat tctaattttt ctaactcttc tttatttcca
     2521 gtaatataat gagctttttg ttttatgaca tcaatttctt catctataat aatttttccc
     2581 tcatttaata ttattacatg ctccagtaaa tcatctattt catcaatcaa atgagttgat
     2641 attattattg tccttggatt ttttatataa ctatctaaaa ttatgttata aaactcttgt
     2701 ctatttactg catccagacc aattgttggc tcatcaaata tggtgatagc tgaattagaa
     2761 caaataccta ttatatttga aagtatagtt ttcatacctc ttgacagttg tttatacttt
     2821 tttttagtat ttaaatcaaa atatttgcat aattcttctt ctaagccttt atcataactt
     2881 ttataaaaac tagaagaaaa ttcaaatatc tgacctactt taaaatctgg actataaaat
     2941 tctttttccc tgacgataca aacttcttcc aaaactttta tatcttcttt tgggttcttt
     3001 cctaatattt ctatctcacc ttcattttga attaactgat ttaccattat atttaataat
     3061 gttgtttttc cagctccatt cttacctaat aaaccatata tcttattttt ttcaaagttt
     3121 agagacatat tattaaaaat attttgcttc ttaaaactgt gagataaatt ttctatctta
     3181 atagaactac tcatatatta atcctccttg aaattaatta taatatcaac caattcttct
     3241 ctatttattt ctaatttgtt agcttcttga agtaggtttt ttacaaaatt atgtttaaaa
     3301 ttttcttttc tagcttcttt tataatagtt tttgcaccat ctgatacaaa catccctatc
     3361 cccctcttct tatataaaat atttttatca accaatatat ttataccttt taatactgta
     3421 gctggattaa ttttataaag ctttgaaagc tctgttgttg atggaacctg ttcttcttct
     3481 tttattccat ttgatagtat ctcatcttca attgctcgtg ctatctgaat aaatataggt
     3541 tcttcattat ttaatactaa tttcataggt tcctcctttc ttcatcagtt agttactcaa
     3601 ctaattaact atataactta taatatcttg aaaaatattt tctgtcaata ctaaataaaa
     3661 aacagaggga aaattccctc tgttttttgt tctttatatt tataattaaa ttttgatttt
     3721 tgtaatccta tcccataagt taaaaatttt aataatctgt tgtatcttca ttttttaata
     3781 ttttacatat tgagctagtt cctaaccttt ctgctcctgc ctctataaat ttttctgcat
     3841 cagaaattga tttaactccc cctgcagctt ttatttttac attttttcca acatgctctt
     3901 tcataagctt tatatcttct aaagtagctc cacctgttcc cattccagta gatgttttta
     3961 taaaatctga acctgacatt gttactattt cacacatttt tattttttca tcttcatcta
     4021 aataacatgt ctctataatg acttttaata ctttatctcc tatagctttt tttatttctt
     4081 ttatttcttt tcctatatta tcataatctt tattttttat atctgaaatg tttacaacca
     4141 tatcaacttc atctgctcca ttttcaatag catcttttgc ttcaaatact tttgtagcag
     4201 tagtttgata tcccaaaggg aaccctatca ctgtacatat ctttatttta ccttttagat
     4261 attctgatgc tcttttcaca tatgatggtg gtatgcaaac tgatgctaca ctcatatcta
     4321 cagcttcatc acataaaatt ttaatatctt cccatgtagt tgtagctttt aaaatagtat
     4381 ggtctactgt ttttaatata tgtttcataa aatcctccta aatttataat taatataatt
     4441 agattacttt tttaaatgca attttctctt tgcaatattc agtatcatat caacctcttc
     4501 aactttaaaa atagtcatta gtagtgtata aattccacct ccaactataa ctgaaatagc
     4561 tagtgataca aactcattaa atgttcctac tcccaaaatt ccaaatataa atttatacgt
     4621 aaagtatgat aaaacaccca taattaaaga tgcaactacc gatttcaaag ttgcttttat
     4681 tattttatct tgtccaaaat atccaacttt tctctttagg ttcaagaaga gtaacaatat
     4741 acatgcaatt gatgatgaac tagttgcaag agcaagacca ccatgagcca ttggttttat
     4801 taaaactaag tccaatacta tatttacccc tacagatata atcccattta ccataggtgt
     4861 ttttgtgtct tgtaatgaat aaaaaacctt tcccaaaata tctcttaagc caaatgcagt
     4921 cataccaact gcataaaaaa ttaaggcagt tgctgtcatt tgggttgccc tcgcatcaaa
     4981 agcacctctc tcaaaaatga ttctaacaac aggagttgca aaaaaaatag aagcaactga
     5041 aataggaatc atagaaatta ttatcatatt tatactggat ttaactgaac tagtaaactt
     5101 cttttgatta ttttctgcac ttaacttaga taacatagga tacataacag atactattga
     5161 agctgtaaat gtccctgtaa caaaaccatt taatctatct gcataagtaa gtgctggaat
     5221 gcttccttta actaatgttg aggctaaagt tgtatctacc aatgagttca cttgattaac
     5281 agcaacacct ataaatactg gtgaaagtaa tgctagtaat ttaataaggc tatcatcttt
     5341 aaagtttaag tagtataagt atttgtagtt cgttttttta acaaaaaaca tgtaaaagag
     5401 cagttgaact accatagcaa ccacagcacc aattggaagt atgtatggtc caaatacggt
     5461 acttaacatt attgaaataa ttattactat attatagggt atacttccaa atccaactac
     5521 aataaagttc tctttaattt gtaaaaatgc tgacataaca ctcgtaatac ctatgaatat
     5581 tattcctgtt ctgaacctcc catgactaaa gtcacagggt tcctgcttca tagaattttg
     5641 catactaaaa tatgtcttac taattctcca caggctatcc ccgtagttcc tacggttctt
     5701 acatacgtta tttattttga attgttatta gtcttaatcc ttcttttaat atatttatac
     5761 ttgcatttat atctctatcg tgagtttcat tacaactagg acaaatccat tctcttatat
     5821 ttaagttctt aacttcctcg tttttatacc cacatttatt gcatatttgt gaacttgcaa
     5881 agaatttacc tacctttaca atttgtctac cataccaatt agccttgtat tctaattgac
     5941 gaataaactc tgaccatgat acatcagaaa ttaagcgaga tagtttacga tttctaatca
     6001 tattctttac ttgtaaatct tctatgcaaa taatatcgtt ctcttttatt aatttagtag
     6061 atagtttttg tagaaaatca tttctttgat tagctatatg ttcttgaagt cttgcaactt
     6121 ttaatcttgc tttatttcta tttaaactac cgattgtttt tctcgataat tctctttgta
     6181 atttagcaag tttgtttaat gacttcttaa gatacttagg attttctata aattctccac
     6241 aacttgagat acaaaattct ttaattccca aatctaaacc tatatgatta ttagtatttt
     6301 caaatacttc aatatctaca tcaatacagc ataatgatac ataatatcta ccacttggtt
     6361 ctttagatat agtagcatta agtattctcc ctttaggtac ttgtttatcc cttatcttaa
     6421 ccataccaag tttaggtagt
//
LOCUS       DAELLV010000203         6407 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.203, whole genome shotgun sequence.
ACCESSION   DAELLV010000203 DAELLV010000000
VERSION     DAELLV010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6407
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.203"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..356)
                     /locus_tag="KPD58_003013"
     CDS             complement(<1..356)
                     /locus_tag="KPD58_003013"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0266745.1"
                     /translation="MYSKIAIKNVRKSFKDYSIYFLTLTLVICIFYSFNSMESQKALM
                     EMSTSKKPDLEMFKNMISYISVFVSAILCTLILYINNLLIKKRNKKLGIYMTLGMGKI
                     EMSKILVFETIIVGIVS"
     gene            complement(346..819)
                     /locus_tag="KPD58_003014"
                     /pseudo
     CDS             complement(346..819)
                     /locus_tag="KPD58_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014521021.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1525..2418)
                     /locus_tag="KPD58_003015"
     CDS             complement(1525..2418)
                     /locus_tag="KPD58_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HBG0266746.1"
                     /translation="MKAEFLTPVVTIFDEEGNLDKEGNKKVYDHLIDGGVDGLVIMGS
                     TGEFFNMSMDMQKELIDLVTSHVNKRVKVFIGTSRMCINESIELSNYAHNSGADGVMI
                     ISPYYFSLDDDSIELFYSEIAKNTEAEIYLYNYPDRTGYDLSPELTLRLARKHKNIVG
                     YKDTVTLMGHTRDLINTMKIEFPEFKVYSGYDENFIHNLMSGGAGCIGGLSNLIPEVC
                     SKWAEAYNNRDMEKVIEIQKYINKAMDFYLITNPFIPAMKKAMNIRGLQISDYMTAPF
                     VKPNEKQVCEIKKLMNELNIC"
     gene            complement(2498..3400)
                     /locus_tag="KPD58_003016"
     CDS             complement(2498..3400)
                     /locus_tag="KPD58_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBG0266747.1"
                     /translation="MKFVSFTEGENECKRTGVFSKDELFIIDVNSLNLSRNFKDLNEL
                     IQNVSSEDIIKLKTIINESTFYNYDVFKRERCTVHVPIEKPIHDILCVGVNYKDHLEE
                     TQTHFDDSFEEPQKTVYFTKRVTKAIGPEDEIDGHFEINNQLDYEVELGVVIGKTGVN
                     IRKEEVEDYIFGYTVINDISARKLQKEHVQWFRGKGLDTFTSLGPCILHKSAVPFPIK
                     LKVCSRVNGEERQSSNTGLFISDVSDIVSELSKGMTLEAGDIIATGTPSGVGMGFSPP
                     RYMSSGDVVECEIESIGILKNYIK"
     gene            complement(3450..4610)
                     /locus_tag="KPD58_003017"
     CDS             complement(3450..4610)
                     /locus_tag="KPD58_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBG0266748.1"
                     /translation="MKFYGYKRPDGRVGIRNHILILPTSVCASDTTRIIASQVQGAVT
                     FNNQNGCSQVHSDQQLTMDVMAGYAANPNVYGVIVVSLGCENCQNDLVVDAIKERTNK
                     PIKTLVIQEEHGTLKTIEKAVRYAREMAQEASLLRKEEFPISELILGTECGGSDPTSG
                     LAANPLIGELSDKLVDLGATSILSETTEFIGAEHILARRAVNEEVKEKILHIVHRYEN
                     SLKLVGEEVREGNPSPGNIAGGLTSLEEKSLGCIHKGGHRQISEVYDYAKQIDKKGLV
                     IMDTPGNDASSVAGMVAGGAQIVVFSTGRGTPSGNPIAPVIKITGNKITFANMEDNMD
                     FDASPVIYGPQTMEELTDDLLNMVVDVANGKQSKAESLGYTEMAIARVCNYV"
     gene            complement(4766..5071)
                     /locus_tag="KPD58_003018"
     CDS             complement(4766..5071)
                     /locus_tag="KPD58_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBG0266749.1"
                     /translation="MINAVIIDEKDNVAVAIEAIAKGSEISFKLKDKTVKTITALDDI
                     TIYHKLAIKDMAKGDKVTKYGEHIGEANQEIKSGQHVHIHNVRSVREDLDKQVMTNI"
     gene            complement(5169..6143)
                     /locus_tag="KPD58_003019"
     CDS             complement(5169..6143)
                     /locus_tag="KPD58_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HBG0266750.1"
                     /translation="MIMDFIKKIPAGMMIVPMFIAAFINTFCPDIVQIGSFTTAVFSS
                     VGSATIIGVQLVCLGTQLQFREMPKVLKRGGILLGSKFAIGALIGILIGKLFGMDGVL
                     GLTTLAVISAVTNSNGSIYLSLMTSYGDETDCATMSLLTLNDGPFFTLIALGTSGLAN
                     VPLLSLLAAIVPILVGMIIGNFDKKMKEFLEPGCNLLVPFVGFALGSSINLASILKGG
                     LSGVLLGLISVFVGGIFIVFCDKFIGKRPGYAGWAVATTAGNAIAVPAAVALVDPSWQ
                     PYVATATTQVAAAVVVTAILVPLITSWWAKKYGCPKFPKQNEDGTITA"
BASE COUNT         2050 a         1152 c          675 g         2530 t
ORIGIN      
        1 gagactattc caactattat agtttcaaaa actaatattt tcgacatttc aatttttccc
       61 attcctaaag tcatataaat tccaagtttc ttatttcttt ttttgattaa taagttatta
      121 atataaagaa ttaaagtaca taaaatagct gatacgaaca cagatatata tgaaatcata
      181 ttcttaaaca tttctaaatc aggctttttt gaagtactca tttccattaa agctttttga
      241 gattccatag agttaaaact atagaaaata caaattacta atgttaaagt taaaaaatat
      301 atactataat ccttaaagct ttttctaaca tttttaatcg ctattttaga atacattgtt
      361 atcatctcct ctgattatag ctatttcttc aattattcta ttaaaagact cttttattga
      421 atcagttcgt cttgctaact ctataaatat ttttctgtat tttataaata agtttctatg
      481 agtataacta gctataaaag catcattagt tagcattaaa attgttgttt ttaaatcttg
      541 atttaaactt tcaaaactct caagtaataa tcttgatgct tttgaatcaa atttatattg
      601 ttttcccttt aattgttaat gtcaatgcta tattttcata agctgttaaa gtatataaaa
      661 gattaaaatt ttggaatata aatcctaatt cattttgtct aaatttatct aagtttttga
      721 attttaatct tgtaatatca ttattatcta ttgcttttgt aatattatct ttgttttcat
      781 aatacttttc catttttcaa cacttaatac atttttcatg ccttatttct cctttaaatt
      841 tcttatatat ctattgtaat ttttaattta atttttttaa attgatttac atttcattaa
      901 tatttcactt ttgtagtaat tgtatattgc tacctataat tactaatata aaatgttcaa
      961 gaccaacaga ttttcttttg tgctttacta gaatcgtaaa atttacccaa ttctagaaaa
     1021 acacaaaaaa tggaaggcaa tgccttccct ctaaaattta ttttattcta attgtatttt
     1081 tctatatgtt ctttcaaaat cattggtatt aaatcattct tactatagtg ctttgtatca
     1141 caaaattatt gccaatcttg atatgtaaat tcatttatct ttatactttt aagcttgaag
     1201 tctaagtgtt tatcatttat tagattaatt attacatgat ttgtagtagt ttatatgtgg
     1261 ctcttattgt taacttatac ttatctaaaa atgtttgtaa aactccaaga ttatttttaa
     1321 tagataaagg ctattgcttt aatatttgtg ctaactttta aactaatctg ctatcttagt
     1381 attatttaat ttttattaac ataattgtaa tttattgttg accacttttc aaagttgatt
     1441 taacatatat aaaaagagtg tttaaaatga ttataaaaat cagtttaaac actctttttt
     1501 tgctcatata tcttacttag taagttaaca tatatttaat tcattcatca attttttaat
     1561 ttcacatact tgtttttcat ttggttttac aaatggagca gtcatataat cacttatttg
     1621 taaccctctg atattcatag cttttttcat tgctggtata aatggattgg ttattaagta
     1681 aaaatccatt gctttattaa tatatttttg aatttctata accttctcca tatctctatt
     1741 attgtaagct tcagcccatt tggagcaaac ttctggaatt aaatttgata acccccctat
     1801 acaacctgct cccccagaca ttagattatg tataaagttt tcatcataac cactataaac
     1861 cttaaattct gggaactcta ttttcatagt atttattaaa tctctggtat gtcccatcaa
     1921 agttactgta tccttatatc caactatgtt tttatgtttt cttgcaagtc ttaatgtaag
     1981 ttctggactt aaatcataac cagttctatc tggataattg tataaatata tttcagcctc
     2041 tgtattctta gctatttcac tataaaataa ctcaatacta tcatcatcaa gagagaaata
     2101 ataagggctt attatcataa caccatctgc tccactatta tgtgcatagt ttgaaagttc
     2161 tatagattca tttatacaca tacgacttgt acctataaac acttttactc tcttatttac
     2221 atgactagtt actaaatcta taagttcttt ttgcatatcc atagacatat taaagaactc
     2281 tccagtactt cccattatga ctagtccatc tacacctcca tctataagat ggtcatacac
     2341 ttttttatta ccttctttat ctaaatttcc ttcttcatca aatattgtaa caactggtgt
     2401 caaaaattct gctttcataa tttaaactcc taaaatttat tttaatatgt tcattaatac
     2461 atcagattta gctttaatat taattatatt aaaacagtta cttaatgtaa ttttttaaaa
     2521 ttccaatact ctcaatctca cattcaacta catctccact actcatatat cgaggaggtg
     2581 aaaatcccat ccctactcca gaaggtgttc cagtagctat aatgtctcca gcctctaagg
     2641 tcataccttt tgaaagttct gaaactatgt ctgaaacgtc tgatatgaat aatcctgtat
     2701 tagaactttg tctttcttca ccatttaccc tactacatac tttaagtttt ataggaaaag
     2761 gaactgcact tttatgtaaa atacatggac ctaaagaggt aaatgtatct aatccttttc
     2821 ctctaaacca ttgaacatgt tctttttgca actttctagc agaaatatca tttataacag
     2881 tgtaaccaaa gatataatct tctacctctt cttttctaat attaacacct gttttaccta
     2941 ttactactcc aagttcaact tcatagtcta gttgattatt tatctcaaaa tgaccatcta
     3001 tctcatcttc tggacctatt gcctttgtca cacgttttgt aaaataaacc gttttttgtg
     3061 gttcttcaaa actatcatca aagtgagttt gagtttcttc aagatggtct ttatagttca
     3121 ctcccacaca taagatatca tgtattggtt tttctatagg aacatgtacc gtacatctct
     3181 ctcttttaaa aacatcataa ttatagaatg tactttcatt tataatagtt ttcaacttaa
     3241 taatatcttc actagataca ttttgaatta attcatttaa atccttaaaa tttctactga
     3301 gatttaaaga gttaacatct ataataaata attcatcttt agaaaaaact ccagttcttt
     3361 tacattcatt ttctccttca gtaaaactta caaacttcat aatctcaccc tttattaaaa
     3421 ttctatgtat ttattttctt acttaaagat tatacatagt tgcatactct agctattgcc
     3481 atttcagtat atccaaggga ctctgctttt gattgttttc catttgctac atctacaacc
     3541 atatttaaca aatcatctgt taattcttcc attgtttgtg gaccatagat aactggacta
     3601 gcatcaaaat ccatattatc ttccatattt gcaaaagtta ttttatttcc agttattttt
     3661 attactggtg ctattggatt tcctgatggt gttcctcttc ctgtactaaa taccactatt
     3721 tgtgctcctc ctgctaccat accagcaaca gatgaagcat catttccagg ggtatccatt
     3781 ataacaagac ctttcttatc aatttgtttg gcataatcat atacttcact tatttgtcta
     3841 tgaccacctt tatgtataca accaagtgat ttttcttcta aacttgttaa accaccagct
     3901 atatttccag gagatggatt accttctcta acttcttctc caaccaattt taatgaattt
     3961 tcatatctat gtactatatg taatattttt tcttttactt cttcatttac tgctctccta
     4021 gctagtatat gctctgcacc tataaattca gtagtttcac ttaaaattga tgttgctcct
     4081 aaatcgacaa gtttatcact taattcacct attaatggat ttgctgctaa tccacttgtt
     4141 gggtcagacc caccacattc agttcctagt ataagttcag atattggaaa ttcttctttt
     4201 ctaagcaaag aagcttcttg tgccatttct ctagcataac gaacagcttt ttctatagtt
     4261 tttaaagttc catgttcttc ttgtattaca agagttttta ttggcttatt tgttctctct
     4321 tttattgcat caactaccaa atcattttgg caattttcgc atcccaaaga tacaactatt
     4381 acaccatata catttggatt tgctgcataa ccagccataa catccatagt taattgttgg
     4441 tctgaatgta cttgtgaaca tccattctga ttattaaagg taactgcccc ttgaacttgt
     4501 gatgctatta ttctagttgt atctgatgca cacacactcg taggcaatat taatatgtgg
     4561 tttcttatac caactcttcc atctggtctt ttatatccat aaaatttcat actgatttcc
     4621 tcctaaaata gtctatatac tttctataat ttttatatat tataattttt attacactca
     4681 ctaatcaaac agctaaaaac ctatttaaaa actatttgac actaaaagca atgaatacaa
     4741 cttttataac tctataaata ttattttata tattagtcat aacttgttta tccaaatcct
     4801 ctctaacact tctaacatta tgaatatgta catgttggcc actttttatt tcctgatttg
     4861 cttctcctat atgttcacca tatttagtaa ctttatcacc tttggccata tcttttatag
     4921 ccaatttatg ataaatagtt atatcatcta gtgctgtaat cgtttttact gttttgtctt
     4981 ttaatttaaa actaatttca ctacctttag ctattgcttc aatagctact gcaacattat
     5041 ctttttcatc aattatcact gcatttatca ttattcttgc tctcctttat aaataatata
     5101 attttattaa tatcctaaaa tctaattaaa gaatttatta ttttaccaac aactcactgt
     5161 aatataaatt aagctgttat tgtaccatcc tcattttgtt ttgggaactt aggacatcca
     5221 tacttcttag cccaccaact agttataaga ggtactaata ttgcagtgac aactactgcg
     5281 gctgccactt gtgttgtagc tgttgcaaca tatggttgcc atgatgggtc aactaatgca
     5341 acagcagcag gtacagcaat tgcatttcca gcagttgtag ctacagccca tcctgcatat
     5401 cctggacgtt ttcctataaa tttatcacag aatactataa atatacctcc tacaaataca
     5461 gatattaatc caagtaatac tcctgataaa cctcctttta gtatacttgc aagatttatg
     5521 ctactaccaa gtgcaaaccc tacaaaaggt acgagtaaat tacaccctgg ctctaaaaac
     5581 tctttcattt ttttgtcaaa atttcctatt atcataccaa caagtattgg cactattgca
     5641 gccaataatg acaacaatgg tacatttgca agacctgaag tcccaagagc tattaatgta
     5701 aaaaatggtc catcatttaa agtaagtaaa ctcatagttg cacaatctgt ttcatctcca
     5761 tatgaagtca tcaatgaaag atatatacta ccattactat ttgtaactgc acttataaca
     5821 gccaatgttg tcagaccaag gacaccatcc attccaaata gctttcctat aagtatacct
     5881 atcaaagcac ctatagcaaa tttagaacca agaagtatac cacctctctt taaaactttt
     5941 ggcatttctc taaattgaag ctgagttccc aagcatacca gttgaacccc aattatagtt
     6001 gcagacccaa cacttgaaaa tactgctgtc gtaaacgaac ctatttgtac aatatctggg
     6061 caaaaggtat taatgaaagc tgctataaac attggcacta tcatcatacc tgcaggaatt
     6121 tttttaataa aatccattat cataaagtta atcctcctaa aacatgatat ttaattactc
     6181 ttattaagtt tttagcttta attctaacgg ccgtttcggt tcgtatttaa gaactaagtt
     6241 tttaattacg aatctttgaa ttaatgatag cattttccta tttatgagtc aataaaaaaa
     6301 ataaattttt gtcaaaattt tttcaaactt tctgataata ccttgtatat tatcaaatga
     6361 cttccttata tgcaaaaata cagcgtattt aaaattatat agtattc
//
LOCUS       DAELLV010000204         6386 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.204, whole genome shotgun sequence.
ACCESSION   DAELLV010000204 DAELLV010000000
VERSION     DAELLV010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6386
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.204"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            12..1808
                     /locus_tag="KPD58_003020"
     CDS             12..1808
                     /locus_tag="KPD58_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate oxidoreductase"
                     /protein_id="HBG0266751.1"
                     /translation="MGIEYFSKLLESSTEKELLLSKKSNNISTARLISFLIVIAGLAI
                     GFYNKNIVGIFVGVLSIIIFIALLVIHSKVKEEETYFKSKSEVLNKYIKRFGDGWKEF
                     KIDGKEYLKDENLQAKDLDLFGRASLYQYICVANTSYGKNFLAKYLWNENPDENVILE
                     RQRAIKELLSKDDFSIHIQTLSNIIGKEQKNNSDSSIESFIEYGENKNVYIPKWMHIF
                     TWGLPTATILSFIFCMLGFLPVLPVFLLFIIQLGFSGFGYPRLMQTLAPLFSFSRSIQ
                     VYEKMFEVLEKETFESAYLKELQDKLSKGSGVSRGVKQLNSIGNAVNLRYNQILYIIA
                     CGVLMWNYHCAEALERWKGIYGNQIRDWFESIGEFEALISLTVISHVKENTCFPVIKY
                     EDTPRLKVEEVYHPLIAEKSVIANSIQLNSQTCIITGSNMSGKTTFLRSIGVNLVLAY
                     AGAPVCAKSFDATCMAIFTSMRIQDDVSQGISTFYAEILRIKSMIQYSLKELPMLVLV
                     DEIFKGTNSADRIIGASEAVKKLSKPWVISMVTTHDFELCDLSGSGDVEVVNYHFSEY
                     YVDDKIHFDYKIKDGRCKTTNAKQLMRMAGIL"
     gene            complement(1948..3729)
                     /locus_tag="KPD58_003021"
     CDS             complement(1948..3729)
                     /locus_tag="KPD58_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HBG0266752.1"
                     /translation="MSLVNLTINGKHVSAPSGTSILDAAKLINIKIHNLCHLHMNEID
                     KLDTCASCRVCMVETERGLVPACGTVIKEGMKVQTNSAKALNARRTIVELLLSDHPQD
                     CFVCEKNGDCELQTIAADLGVRKIRYQGSKSFAGKDTSTKSLVKDHSKCILCRRCETV
                     CNDIQTVGALSGVNRGFNTLVSTFFNSDMVETECTFCGQCISVCPTGALTEVDNVPKL
                     WDVLNKKEKTIVVQVAPAVRVAIGEEFGLEPGSISTGKMVAALKALGFEHVFDTNFGA
                     DFTIMEEATEFIERIQKGENLPILTSCCPAWVNFLEHNYPDKLNLASSCKSPQGMFGS
                     IAKNYYAPKILGINPEELYVVSVMPCVAKKYEASREELSESGILDVDLSITTRELAKM
                     IKEAAIDLPNLQDQDFDNPLGKSTGAASIFGASGGVLEAALRTSYEKITNKTLDNVNF
                     TNVRGLKGIREASIDVDGTTVNVCIVNTLKNARKIMDKVRSGECKYHIIEVMACPGGC
                     VGGAGQPYHHGNTEIVDRRANALYEIDRNKAIRKSHENPDLQAIYKDFFGEPNSDVAH
                     KYLHTHYFDKSCVYGECPQECACEEAK"
     gene            complement(3752..5638)
                     /locus_tag="KPD58_003022"
     CDS             complement(3752..5638)
                     /locus_tag="KPD58_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HBG0266753.1"
                     /translation="MRKVNSFDELKVLADELKPNLSLRKNYSKENVLRRELLVCCDTG
                     CTSSNSLEIVSELENEIKKSGIQDKVSVRLTGCFGFCAQGPIVKVYPDNVFYVKVEPS
                     DAEKIVQSHLIRNTVVEELLYEEESLSKKVEEQEEMSFYKKQLRIALKNCGLIDPDSI
                     EEYIANDGYLALGKCLTSLTPQEVIAEVKTSGLRGRGGAGFPTGSKWEAASKNPAGPT
                     DKKFVVCNADEGDPGAFMDRSVLEGDPHSVLEAMAICGYAIGSDTGYIYIRAEYPKSI
                     ERLKVAIAQAEKYGLLGENILGTGFNFKLELKYGAGAFVCGEGTALMHSIEGRRGEPR
                     MKTYSSSKSGLWKSPTCLNNVETFANIPAIILKGGDWFANLGTEDSSGTKVFALGGKV
                     ENVGLVEVPMGTTLRDIVFEIGGGIPEGKKFKAVQTGGPSGGCIPTEHLDTPIDFGSL
                     SSIGSMMGSGGMLVLDESDCMVDIAKFFLEFTVEESCGKCTPCRIGTTRLLEILTKIT
                     EGNGTLQDLDDLENLAETIQTASLCGLGKAAPNPVLSTLQYFKDEYIAHVVDKKCPAG
                     KCQNLLSYFITDDCKGCTKCSRVCPAGCITGSVKEQHTIDTSKCLKCGACIDNCTFNA
                     IIKK"
     gene            complement(5655..6152)
                     /locus_tag="KPD58_003023"
     CDS             complement(5655..6152)
                     /locus_tag="KPD58_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HBG0266754.1"
                     /translation="MCDFISHNKQLFDELDIFIDSLATKEGALIQVLHEAQGIFGYLP
                     KEVQLHVARKLGVAPAKVYGVVTFYSYFTTEPVGKYKISVCLGTVCFVKGADKILSAF
                     EKQLGIKVGETTSDFKFSLEGLRCLGACGLAPVVTVNGKVYGKVKPDQVSEILDTYRE
                     LELNC"
BASE COUNT         2189 a         1101 c          948 g         2148 t
ORIGIN      
        1 tggaattttt tatgggaata gaatatttta gtaaactttt agaaagtagt actgaaaaag
       61 aattgctctt aagtaaaaag tcaaataata taagtacagc aagattaata tcttttttga
      121 tagtgatagc agggcttgct attggatttt acaataaaaa tatagtaggt atttttgttg
      181 gagttttatc aataataata tttatagcac tcttagttat tcatagtaaa gtaaaagaag
      241 aagaaacata ttttaaaagc aagagcgaag tattaaataa gtatattaag agatttggag
      301 atggttggaa ggaatttaaa atagatggaa aagaatactt aaaagatgaa aacttgcaag
      361 caaaagattt agacttattt ggaagagcat cattgtatca atatatctgt gtagctaata
      421 catcttatgg gaaaaatttt ttagccaaat atttgtggaa tgaaaatcca gatgagaatg
      481 taattttaga aagacagaga gctataaaag aacttttatc aaaagatgat ttttctattc
      541 atattcaaac actcagtaat ataattggta aagagcaaaa aaataattca gatagtagta
      601 tagaatcgtt tatagagtat ggagaaaata agaatgtgta tataccaaaa tggatgcata
      661 tttttacttg gggtttacca actgcaacaa ttttatcttt tatattttgt atgttaggat
      721 ttttacctgt tttacctgtt tttttacttt ttataatcca gttagggttt agtggttttg
      781 gatatccaag acttatgcaa actcttgctc cattattttc atttagtaga agtatacaag
      841 tatatgaaaa aatgtttgag gttttagaaa aagagacgtt tgagagtgca tatttgaaag
      901 aattacaaga taaactttct aagggaagtg gagtttcaag aggtgttaaa caattaaatt
      961 caataggaaa tgctgtaaac ctaaggtata atcagatact atatataatt gcctgtggtg
     1021 ttttgatgtg gaactatcat tgtgcagaag ctttggagcg atggaaaggt atatatggaa
     1081 atcaaattag agattggttt gaatctattg gagaatttga agctctaata agtttaacag
     1141 ttatttctca tgtaaaggaa aatacatgtt ttccagtaat aaaatatgaa gatactccaa
     1201 gattgaaagt tgaagaggta taccacccat taatagcaga aaaaagtgtc atagcaaatt
     1261 cgatacaatt aaattctcag acttgtatta tcacaggttc aaatatgtct ggaaagacaa
     1321 catttttaag aagtatcggt gtaaacttag tgctggcata tgcaggtgct ccagtatgcg
     1381 ctaagagttt tgatgcgacc tgtatggcta tatttacttc tatgagaata caagatgatg
     1441 taagtcaagg aatatcaact ttctatgctg agattcttag aataaaatca atgattcaat
     1501 atagtttaaa agaactgcca atgttggttc tagtagatga aatttttaag ggaactaact
     1561 ctgctgatag aattattgga gcaagtgagg cagtaaagaa attatcaaaa ccctgggtta
     1621 taagtatggt gacaacacat gactttgagc tatgtgacct ttcaggtagt ggagatgttg
     1681 aagttgtaaa ctatcatttc tcagagtact atgtagacga taaaattcat tttgactata
     1741 agataaaaga tggaagatgt aaaactacaa atgcaaaaca acttatgaga atggcaggta
     1801 ttctttaatt aatagatagg ttaattaaag aataagtcta tttagaaata taatttaatt
     1861 tgtgaaatta ataaggattg tcttaaatta cgtaaaagta ttttaagaca atcctttttt
     1921 aagaataaat tttatatttt gttatgacta cttagcttct tcacaagcac attcttgtgg
     1981 acactctcca tagacacaac tcttatcaaa gtaatgagta tgaaggtatt tatgggctac
     2041 atcactattt ggttctccaa agaagtcttt ataaatagct tgtaaatcag gattctcatg
     2101 agatttacga atagccttat ttctatctat ttcatataac gcatttgcac gtctatcaac
     2161 tatttcagta ttaccatggt gataaggttg accagcacct ccaacacatc caccaggaca
     2221 tgccataact tctatgatat ggtatttaca ttctccactt ctgaccttat ccataatctt
     2281 tctagcgttt ttaagtgtat ttacaataca cacattaact gtagttccat caacatctat
     2341 acttgcttcc ctaattcctt taagacctct aacattagtg aagtttacat tatctaaagt
     2401 cttattagtt atcttctcat atgaagtacg tagggcagct tctaaaactc caccacttgc
     2461 tccaaatata gatgcagcac ctgtagattt tcctagagga ttatcaaaat cttggtcttg
     2521 aagattaggt aaatcgattg cagcttcttt tatcatctta gctaactctc tagtagttat
     2581 agaaaggtca acatctagta ttccagattc acttaattct tctcttgaag cttcatattt
     2641 tttagctaca caaggcatta cagatacaac ataaagttct tcaggattga ttcctaatat
     2701 tttaggtgca tagtagtttt tagctataga accaaacata ccttgaggag acttacagct
     2761 agatgctaag tttaacttgt ctggataatt gtgctctaag aagtttaccc atgcaggaca
     2821 acaacttgtt aatataggaa gattttcacc tttttggatt ctttctataa attcagtagc
     2881 ttcttccatt atagtaaaat ctgcaccaaa gtttgtatca aatacatgtt caaatccaag
     2941 tgcttttaaa gcagctacca ttttccctgt agagattgac cctggttcta atccaaactc
     3001 ttcaccaata gcaactctta cagcaggagc aacttgcact actatagtct tttctttttt
     3061 atttaaaaca tcccaaagtt taggtacatt atctacttct gttaatgctc cagttggaca
     3121 gacagatata cattgaccac agaatgtaca ctctgtttca accatatcag agttaaagaa
     3181 tgtactaact aaagtattaa atccacgatt aactccagat aatgctccta ctgtttgtat
     3241 gtcattacat acagtctcac atcttctaca taatatacac ttagaatggt ctttaactaa
     3301 agatttagta gaagtatctt tacctgcaaa agatttagaa ccttgatatc ttatttttct
     3361 tactcctaaa tcagcagcta tagtctgtaa ttcacaatct ccattttttt cacagacaaa
     3421 acaatcttgt ggatggtctg ataacaataa ttcaacaata gttctacgag catttaatgc
     3481 tttagcacta ttagtctgaa ctttcatacc ttcctttata actgttccac aagctggaac
     3541 taatcctctc tctgtctcaa ccatacaaac acgacaagaa gcacaagtgt caagcttatc
     3601 tatttcattc atatgtaaat gacaaaggtt gtgtattttt atatttatta atttcgcagc
     3661 atctaagata gaagttccac taggtgctga aacgtgtttg ccattaatag ttaaattaac
     3721 taaactcatt gttattcctc ctccaaataa gttatttctt gattatagcg ttaaatgtac
     3781 aattatctat acaagcacca cattttagac acttagaagt atctattgta tgttgttcct
     3841 taacagaacc tgttatacaa ccagcaggac acactctaga acattttgta caacccttac
     3901 agtcatctgt tataaagtaa gaaagtaagt tctgacattt accagcagga catttcttat
     3961 ctacaacatg agctatatac tcatctttaa aatattgaag agtacttaaa acagggtttg
     4021 gagcagcttt accaagacca cataatgaag cagtttgtat agtttcagca aggttttcta
     4081 ggtcatctaa atcttgtaga gtaccattac cttctgtaat tttagttaat atttctaata
     4141 atcttgtagt acctatacga caaggtgtac atttaccaca agattcttca acagtaaact
     4201 caaggaagaa cttggctata tctaccatac agtcagattc atcaagaaca agcataccac
     4261 cagagcccat cattgaaccg atagaactta atgaaccaaa gtctatagga gtgtctaaat
     4321 gttcagttgg tatacatcca ccagatggtc cacctgtttg aacagcctta aatttcttac
     4381 cttcagggat tcccccacca atttcaaata caatatctct taaagtagta cccattggaa
     4441 cttcaacaag tcctacattt tctacttttc caccaagagc aaagacttta gtaccactag
     4501 aatcttcagt acctaagttg gcaaaccaat ctccaccctt aagtataatt gcaggtatgt
     4561 tagcaaaagt ctcaacgtta tttaagcaag taggagattt ccatagacca ctcttagaag
     4621 aactataagt tttcattcta ggttcaccac gtctaccttc aattgaatgc attagcgctg
     4681 taccttcacc acaaacgaaa gcacctgcac catattttaa ttctaattta aagttaaatc
     4741 cagtgcctaa gatattttcg ccaagtaaac cgtatttttc agcttgagct atggctactt
     4801 ttaatctttc tatagatttt ggatattctg ctcttatgta tatgtatcct gtatcagaac
     4861 ctatagcgta tccacatatt gccatagctt ctagtacact atgagggtca ccttcaagta
     4921 cagatctatc cataaatgca cctgggtcac cttcatcagc attacataca acaaactttt
     4981 tatctgttgg accagctgga ttttttgaag cagcttccca tttagagcca gtagggaaac
     5041 ctgcaccacc tctaccacga aggccagaag tttttacctc tgcaataact tcttgaggag
     5101 taagagaagt taaacatttg cctagagcta aataaccatc attggcaata tattcttcta
     5161 tactatctgg gtctataaga ccacaatttt ttaaagctat acgtaactgt tttttgtaga
     5221 aagacatttc ttcttgttct tcaacttttt tacttaaaga ttcttcctca tataataatt
     5281 cctcgacaac tgtatttcta attaagtggc tttgaactat cttttcagca tcagatggct
     5341 caacttttac gtagaaaacg ttatcaggat aaacttttac aataggacct tgagcacaga
     5401 aaccaaaaca acctgtcaaa cgaacactaa ctttatcttg gattcctgat ttctttattt
     5461 cattttctaa ttcactaact atttctagac tgtttgaaga tgtacatcca gtatcacaac
     5521 aaacaagtaa ttctcttctt aatacatttt ccttagaata attttttctt aagctaagat
     5581 taggtttcag ctcatcagct aaaactttta attcatcaaa tgaatttact ttacgcatta
     5641 tttattcacc cctcttaaca attcaattct aattctctat aagtatctaa tatttcgctt
     5701 acttggtcag gtttcacttt accataaact ttaccattta cagtaacaac tggagctaaa
     5761 ccacatgccc ccaaacatct taaaccttct aaagaaaatt taaaatcact agtagtttct
     5821 ccaactttta tgccaagctg tttttcaaaa gcacttaaga ttttgtcagc accttttaca
     5881 aaacatacag ttcctagaca gacactgatt ttatacttac ctacaggttc tgtagtaaaa
     5941 taagaataga atgtaacaac cccatatacc tttgcaggag ctactccaag ttttctagca
     6001 acatgaagtt gaacttcttt tggaagatat ccaaatatac cttgggcttc atgtagaact
     6061 tgtattaacg caccttcctt tgttgcaaga gagtcaataa atatatctag ttcgtcaaat
     6121 aattgcttgt tgtgtgaaat aaaatcgcac ataacaaatc ccccttttct ttttaaagtt
     6181 attatttttc ataaaataat taaccatgag ttgaccccaa cattatgtgg gtgaacaaaa
     6241 ccaaaacaat aaagaaagaa taatatacaa caatttcttt atttataaaa acaatgcaaa
     6301 aaagttacct attaaaaata aaaactatac tagtctatta taatataaaa taattcagaa
     6361 tagttaaaaa aaataaaaaa aatgag
//
LOCUS       DAELLV010000205         6341 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.205, whole genome shotgun sequence.
ACCESSION   DAELLV010000205 DAELLV010000000
VERSION     DAELLV010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6341
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.205"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            67..297
                     /locus_tag="KPD58_003024"
     CDS             67..297
                     /locus_tag="KPD58_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266755.1"
                     /translation="MRLDMDSMLYVLIYMTLLVSIIGSIFYFCKDLLNHKIKYVLIRD
                     IFVNRITYSEERLPKQYIVFQKYDIWRYCSLF"
     gene            354..1004
                     /locus_tag="KPD58_003025"
     CDS             354..1004
                     /locus_tag="KPD58_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBG0266756.1"
                     /translation="MDFISSILQGLLSILFNFTGDFGISIVLITLIVKLVLMPLSLKQ
                     RFSVKKQQELAEKMEYIKEKYKNDTKELEKQIQVSSVESMKSMLGCLIVLVQMPVVYA
                     LYHVCLDMTKEFTTIIIPWIANLGTFDNLFILPCIYTLIMLASNLVNYIPYLKINSQV
                     KFNKQMAVVTTITSLILTVRTPVAIGLYFITSAIYSLIEDICFRIYFNRKNRIVTK"
     gene            1207..1878
                     /locus_tag="KPD58_003026"
     CDS             1207..1878
                     /locus_tag="KPD58_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0266757.1"
                     /translation="MAKILVVEDEKRMQNIIVEYMQKGGYTCITADDGVEALTILKSN
                     NIDLMILDIMMPYLDGFSVCRVSREMTNIPIIILTAKGEEEDKLKGYEYGADDYITKP
                     FSPKVLLAKVNALLRRYTTDIPKNSLSLGKIFIMVASRQVYVEDKLIDLTYKEFELLR
                     LFMENPNQVFSREKLLNCIWGYDFEGNTRTVDTHIKTLRKKLGSEGHHIVTLIRSGYK
                     FEVKE"
     gene            1875..3005
                     /locus_tag="KPD58_003027"
     CDS             1875..3005
                     /locus_tag="KPD58_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0266758.1"
                     /translation="MNSLRKIIEKLKNVDISNWIKQNFGLYSVRKKVFLLSKLSGALI
                     ILFYLVVEEFSINSKFEFWIWFIILVIFIMSIDFLLGKFISEPITSINKSAKSMSQLD
                     FSNPCTVNTNDEFGELSRSLNTMSTNLQQALSDLESANIQLEKDVNKERMLLEQRKEL
                     VDTISHEMKTPLGIIRAYTEGLIDEVDEEKKKNYMNVIIEETDRMNNMIVSLLDLSAL
                     EAGVSKLNPERFDFIEFVETVAGRLLIDIPDANFYFTYDLPEYEVFVVADKMRMEQVV
                     ENLVINAKKHVVYNGNLDLSITCENGLLLFEIYNDGRFIEQDEISKIWSKFYRSVQSQ
                     RTGGSGLGLAIVSQILTMQGLEYGVNNRDRGVEFYFMIPIDE"
     gene            3436..4281
                     /locus_tag="KPD58_003028"
     CDS             3436..4281
                     /locus_tag="KPD58_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0266759.1"
                     /translation="MIKVRELKFSYGKDKQILHGLNFEVKEGEIFGFLGPNGSGKSTT
                     QKILNGVLKGYNGHVSIFGKEVKAYTESLYQKIGVLFEFPYLYTNLSAIDNLEYFSSF
                     YPKNQRRDIYELLDLLEFKKEFINKPVSSYSKGMKQRISMARALVSNPKLLFLDEPTS
                     GLDPSGAVLFRKIIEEERKKGTTIFLTTHNMLDADLMCNNVAFIADGKIMVIDKPKNL
                     KMKNSNNKVEVEFVYNGNRDIEIVDIQELESGITFKYDEILSVHSKEPTLEEVFIKYT
                     GRMLV"
     gene            4281..5309
                     /locus_tag="KPD58_003029"
     CDS             4281..5309
                     /locus_tag="KPD58_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0266760.1"
                     /translation="MYSKFFTLVKKDFWMMIYGKFFVLTLGSLILYSCYINFGYVKFM
                     DEDSYHNNVYLYNPENISMSASPLINQVSSIGELKAKLDEDTSAVCIDMSSSKPKVIL
                     HESIKGVDNYRADYALSLLSSEKDHNSKISDNNAIIQINDKDTSEIKQRKDMTCEILF
                     FEIVAIGFLGIASLLFKEKQMGVMRITGILPVKRSLFILSKIFAFFICDLCFTVLLVV
                     FNIGFLEGIKILPQVLVQASILSIIMSLIGIGCSMLLRDFKQFSLIYLVVAVFITTPI
                     FLSANTSFEIDWIKYHPFYHVYSQLKNAFFMNFSKELVYFVVCSLTIIVLFLLVKYIF
                     NKELVREG"
     gene            5312..6325
                     /locus_tag="KPD58_003030"
     CDS             5312..6325
                     /locus_tag="KPD58_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0266761.1"
                     /translation="MIGLRYQLKSIKKDKMCIISFFLPVVMAILINFMGTIDISSLGE
                     IRFGIVKDTLPVQTEQWLKKYGMVSVYDTKMHLIREIKDIKTDTIGVVRDGKGIKTVF
                     YGNEINAIKNVGKTLPEMFRLKGDLKDVEVTILPKDSILKEFQNVFIAMILITAMFMG
                     CTFNAMNIISEKEDGISFINEVLPQSRSQYIIQKVFLGLLCGCLSSMITVFICLDISI
                     LDMIMMLLLVVFSAFISSLIGLFIGKLSEGLMVGIVYIKIVMILFIGTPLLIYLLGVN
                     TSGIGNLCYIMPSVATFNGIMEIINGNVIIIKDIVILAVHCVVWFMIYIILDKKRKLS
                     FSR"
BASE COUNT         2381 a          620 c         1104 g         2236 t
ORIGIN      
        1 aatgttggaa ttatttagga cttgaaattg tctaaaaata aatacaagtg atgaagaggt
       61 gatgtgatga gattagatat ggatagtatg ttatatgtac taatttatat gacattattg
      121 gtatctatca ttggaagtat attttatttt tgtaaagacc tcttaaatca taaaattaag
      181 tatgttttaa taagagacat atttgtaaat agaattacat attctgaaga acgactgcct
      241 aaacagtata tagtttttca gaaatatgat atttggaggt attgtagttt gttttaattt
      301 gaaatgaatc tattataaat tctaaagttt aaaaataaaa aggagagaat aaaatggatt
      361 tcatttcaag tatattgcaa ggattattaa gtatattatt taattttaca ggtgattttg
      421 gaatttctat tgtacttata actttaatag ttaaattagt acttatgcca ctatcattaa
      481 aacaaagatt ttctgtaaaa aaacagcaag aattagctga aaaaatggaa tatataaaag
      541 aaaagtataa gaatgatact aaagaattgg aaaagcaaat acaagtaagt tctgtagaaa
      601 gtatgaagag tatgctggga tgtcttatag ttttggttca aatgccagta gtatatgctt
      661 tatatcatgt atgtttagat atgacaaagg aatttacaac aattataatt ccatggattg
      721 ctaatttagg tacatttgat aatttgttta tattaccttg tatatataca ttaattatgt
      781 tagcttcaaa tttagtgaat tatataccat atttaaaaat aaattctcaa gtaaagttta
      841 acaaacaaat ggctgtggtg actaccataa caagtcttat actaactgtt agaactccag
      901 tagccatagg tctgtacttt ataacaagtg caatttattc attaattgaa gatatttgct
      961 ttagaatata ttttaatcga aaaaatagaa tagtgacaaa ataatataat tattattaga
     1021 aatattttag cctgctaaca tagaaaaagt tagtaggcta tttttatcta tgatatcaat
     1081 tccattaaga atagtctaaa aaatactatc aaaatgctat aaaaaatact ataaaaaaat
     1141 agttgaaaaa taaataaatt aatataaact aaaataaagc ctacaaaaaa gaggtgataa
     1201 atagaaatgg ctaaaatact agttgtagaa gatgaaaaaa gaatgcaaaa tattattgta
     1261 gaatatatgc aaaagggagg atatacttgt ataactgctg atgatggagt cgaggcactt
     1321 acaatattaa agagcaacaa tatagattta atgatattgg atattatgat gccgtattta
     1381 gatggatttt cagtgtgtag agtttcacgt gaaatgacta atatacctat tattatattg
     1441 acagctaaag gtgaagagga ggataaatta aagggttatg aatatggcgc agatgattat
     1501 ataacaaaac catttagccc aaaagtactt ttagcaaaag ttaatgcttt gttacgtaga
     1561 tacactactg atattcccaa aaattcattg agtttaggaa agatttttat tatggtagca
     1621 tcaagacaag tgtatgtaga agataaattg attgatttaa catataaaga atttgaatta
     1681 ttgcgtttat ttatggaaaa ccctaatcaa gttttttcca gagagaaact tttaaattgt
     1741 atatggggat atgattttga aggaaacaca cgaactgtag atacacatat aaaaacctta
     1801 cgcaaaaagc ttggtagtga aggacaccat atagttacat taattcgttc aggatataag
     1861 tttgaggtta aggaatgaat agtttaagaa agattataga aaaactgaaa aatgtagata
     1921 tttcaaattg gattaaacaa aattttggac tatattcagt tagaaaaaaa gtgtttttac
     1981 tttctaaatt atcaggagcg ttaattatat tattttatct ggttgttgaa gaattttcga
     2041 taaactctaa atttgaattt tggatttggt ttataatact ggttatattt attatgagta
     2101 ttgatttctt attgggaaaa tttatttcag aaccaatcac atctataaat aaatctgcaa
     2161 aaagtatgtc ccaacttgat ttttctaatc catgtactgt aaatactaat gatgaatttg
     2221 gagaactttc taggagcctg aatacaatgt ctacaaatct acaacaggca ttatcagacc
     2281 tagaaagtgc aaatattcaa cttgaaaagg atgtaaataa ggaaagaatg ctattggaac
     2341 aaagaaagga gttggtagat acaatttcac atgaaatgaa gactccactt ggcattatac
     2401 gtgcgtatac agaggggctt atagatgaag ttgatgaaga aaaaaagaaa aattatatga
     2461 atgttataat agaagaaact gatagaatga acaatatgat tgtatcatta ttagacttat
     2521 ctgcattgga agcaggagtt tctaaactta acccagagcg ttttgatttt atagagtttg
     2581 ttgaaactgt ggcaggtcgt cttttaattg atataccaga tgccaatttt tattttacat
     2641 atgatttgcc agagtatgaa gtttttgtag tggcagataa aatgagaatg gaacaagtag
     2701 tagagaatct agttattaat gcaaagaagc atgttgtata taatggtaat ttagatttat
     2761 cgattacatg tgaaaatggc ttgcttttat ttgaaattta taatgatggt aggtttatag
     2821 agcaagatga gatttcaaag atttggtcta agttttatcg tagtgttcaa tctcagcgta
     2881 caggaggttc aggtctaggg cttgcaattg tttcacaaat acttacaatg caagggttag
     2941 aatatggagt gaataatcgt gatagaggag tagagtttta ttttatgatt ccaattgatg
     3001 aataatttag tattaattta taaattataa gtatctgatg taaaatattt ttatttgtat
     3061 acgtaagtat ttaaagagaa taaaataaaa aaacttaatg aaaaagctta atgaaaaagt
     3121 ctaataaaaa gcttaatgaa aagcttaata aaaagtcttg ttttcctgat gaaaacaaga
     3181 ttttttatta agctttttat tatttcactt cgatttcaca agagagacac tctgatttca
     3241 ccaaatagaa ataaaatagg ttaaataaat taaaaaatag aagcgtaatt aaataaggtt
     3301 acaaggggga gtacttatga aatggtatac atggttgact attatattga taatattgct
     3361 ttctattccg cttaaaatta aatttataaa atggtgggat agacgacaaa aaaatgataa
     3421 aggaggttat gatagatgat aaaagtaaga gagttaaaat tttcttatgg aaaagataaa
     3481 cagatattgc atggacttaa ttttgaagta aaagaaggag aaatctttgg attccttgga
     3541 cctaatggtt caggaaaatc cacaacacag aaaatcttaa atggtgtttt aaaaggttac
     3601 aatggtcacg tatccatttt tggtaaggaa gttaaagcat acacagaatc tttatatcaa
     3661 aagattggtg ttttatttga atttccttat ttatatacca atttaagtgc aattgataat
     3721 ttagaatatt tttcgtcttt ttatcctaaa aatcaaagga gagacatata tgaactctta
     3781 gatttgttag agtttaagaa agagtttata aataagcctg tatcatccta ctcaaaaggt
     3841 atgaagcaac gtattagcat ggcaagagca ttggtaagta atccaaagtt gttattttta
     3901 gatgagccta ctagtggact tgacccatct ggagctgtac tatttcgtaa gattattgaa
     3961 gaagaacgaa aaaaaggaac aactatcttt ttgactactc ataatatgct agatgcagat
     4021 ttaatgtgta acaatgtggc ttttattgca gatggaaaga ttatggtaat agataaaccc
     4081 aaaaatttaa aaatgaaaaa tagcaataat aaagttgaag tggaatttgt atataatgga
     4141 aatcgagata ttgaaattgt agatatacaa gagttagaat ctggaataac ttttaaatat
     4201 gatgaaatct taagtgtaca ttcaaaagaa cctacactag aagaagtttt tattaaatac
     4261 acaggaagga tgttagtgta atgtattcta aattttttac cttagtaaaa aaagattttt
     4321 ggatgatgat ttatggtaaa ttttttgtgc tgacacttgg ctctctcata ctttattcgt
     4381 gttatattaa ttttggttat gtaaagttta tggatgaaga tagttatcac aataatgtat
     4441 atctatataa tccagagaat ataagcatga gtgcatctcc attaattaat caagtttctt
     4501 ctataggtga attaaaagca aaacttgatg aagatacaag tgcagtttgt attgatatga
     4561 gttccagtaa gccgaaagta attttacatg aaagtattaa aggagttgac aattatagag
     4621 ctgactatgc attgtcatta ttatcatcag aaaaagacca taatagcaaa atatcagaca
     4681 acaatgcaat aattcaaatt aatgataagg atacatctga gataaaacaa agaaaagaca
     4741 tgacttgtga gattttattc tttgaaattg tagctattgg atttttaggg attgcctctt
     4801 tattatttaa agaaaagcag atgggagtaa tgcgtattac tggtatttta cctgtaaaaa
     4861 gaagtctttt tatattatca aagatatttg ctttttttat atgtgaccta tgttttacag
     4921 ttcttttagt agtatttaat attggatttt tagaaggaat aaaaatactt ccacaagtat
     4981 tagttcaagc tagtatttta tcaattatta tgtctttgat tggaatagga tgctcaatgt
     5041 tattgagaga ttttaagcaa ttttctctta tatatcttgt ggttgctgta ttcataacta
     5101 cacctatatt tctatctgca aatacatctt ttgaaataga ttggattaaa tatcatccct
     5161 tctatcatgt ttatagtcag cttaaaaatg cgtttttcat gaatttttca aaagaattag
     5221 tttattttgt agtgtgttct ttgacaataa tagttctatt tttacttgta aaatatattt
     5281 ttaataaaga attggttagg gagggataat cttgatagga ttacgttacc agttaaagag
     5341 tataaaaaaa gataaaatgt gtataatttc cttcttttta ccagttgtta tggcgatttt
     5401 aataaatttt atgggaacaa ttgatatatc ttcattagga gagatacgtt ttggtatagt
     5461 gaaggataca cttcctgtac aaacagagca gtggttgaag aagtatggta tggtaagtgt
     5521 atatgacaca aaaatgcatt taatacgtga aattaaggat attaaaacgg acacaattgg
     5581 agttgttaga gatggtaaag gaataaaaac tgtgttttat ggaaatgaga ttaatgctat
     5641 aaaaaatgtt ggaaagacat tgccagagat gtttagacta aagggtgatt taaaagatgt
     5701 tgaggttaca atattgccta aagatagtat tttaaaagag tttcaaaatg tttttattgc
     5761 tatgatttta attactgcta tgtttatggg atgcaccttt aatgcaatga atattatatc
     5821 ggaaaaagaa gatggaatat catttataaa tgaagttttg ccacagagta gaagtcagta
     5881 tattatacaa aaagtatttt tgggtctttt atgtgggtgt ttatcttcaa tgataactgt
     5941 atttatatgt ttggatattt caatattaga tatgataatg atgttgttac tcgttgtatt
     6001 ttcagcattt atttcatcac ttataggttt atttattgga aagttgtcag aaggtcttat
     6061 ggtaggaatt gtttatatta aaatagtaat gattttattt ataggtactc ctttgttaat
     6121 atatcttcta ggggttaata ccagtggtat agggaattta tgctatataa tgccttcagt
     6181 tgctacattt aatggtatta tggagattat taatggtaat gtcattatta tcaaagatat
     6241 tgtaattttg gctgtacatt gtgtagtatg gtttatgata tatattattt tagataaaaa
     6301 aagaaagtta tcttttagta gataactcag tataattata a
//
LOCUS       DAELLV010000206         6340 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.206, whole genome shotgun sequence.
ACCESSION   DAELLV010000206 DAELLV010000000
VERSION     DAELLV010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6340
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.206"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            237..1181
                     /locus_tag="KPD58_003031"
     CDS             237..1181
                     /locus_tag="KPD58_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HBG0266762.1"
                     /translation="MKFLRIEDEVIYSKKRYKLKREIDKLFLDEEYFQIEPQLFEEYD
                     EFTTINNKIPEESMVKVVNGKVMVLRADITTSIIKSLVPRWEDGLKLKLFYNSSIYKN
                     KNTVGIKEIRQIGCEYLGEASVEADREVVKLALKILEKYNNNFILEVGSSKYIHGLLE
                     ELNLNKNCENQIKNLLYTKNTHELKVYIEELKIKREVKELLSNILSLQGNLYNVIEKS
                     NKFYCNNKMKQALEELKQVNNLIEECNFLDKARFDLSMITMLDYYEGIMFRGYYPNSY
                     KEILSGGRYDSLTKEYGKEIPAIGFTLSVDELMKYVYK"
     gene            1203..1829
                     /locus_tag="KPD58_003032"
     CDS             1203..1829
                     /locus_tag="KPD58_003032"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HBG0266763.1"
                     /translation="MDYLTIALAKGRIEGESFKKFKKMGLGDSIDTDTRKLIFKDEEN
                     KIIYIHVKPSDVVTYVEKGVADLGIAGKDTILENETDVYEIYDLGFGKCKFAVAGLKG
                     DSIYREDEYLKVATKYPNIAKKYFKEKGQKIEIIKLNGSVELAPIVGLSDVIVDIVET
                     GNTLKANGLEILEDICNISARIISNRASYRFKYEQIQNIIRLFEELDN"
     gene            1863..2912
                     /gene="hisC"
                     /locus_tag="KPD58_003033"
     CDS             1863..2912
                     /gene="hisC"
                     /locus_tag="KPD58_003033"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBG0266764.1"
                     /translation="MREKESIRELKGYEPNHVNCKVKLDANEGSKRLFKYLIKEISDS
                     DIDLNLYPEDSYSDLKESIIDYINISGVNKKNLLVGNGSSEIIDLIIHTFVDKDEVIL
                     SFSPSFSMYSIYSQINGSKFIGVESDENLVINIDNVIEKVKENNPKIVIVCNPNNPTG
                     TILKREEIIKLLDSTNSLVVLDEAYMDFGEESMLSDVFKYDNLIVLRTLSKAFGLAGI
                     RTGYMLSNSSLINSVEKVRPPYNLNSLSDFIATRALRNKDVVKAYIKEVKEEREVLYK
                     EMIGMGIKAYKSQANFILFYSEIENLSQKLIDRGVLIRKFGGKLENYYRVTIGDKEEN
                     SMFVGAIRDILKKEK"
     gene            2915..3493
                     /gene="hisB"
                     /locus_tag="KPD58_003034"
     CDS             2915..3493
                     /gene="hisB"
                     /locus_tag="KPD58_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HBG0266765.1"
                     /translation="MRIWKVERNTLETQILVELNIDGSGKAEIDTGIGFLDHMLTLMS
                     FHGKFDLKVICKGDTYVDDHHSVEDIGIAIGEAFKNALGDKKGIRRYSNIYIPMDESL
                     SMVAIDISNRPYLVFNAKFDTQMIGSMSTQCFKEFFRAFVNESRVTLHINLLYGENDH
                     HKIESIFKAFARALKEGSEIVSNEIASSKGVL"
     gene            3495..4100
                     /gene="hisH"
                     /locus_tag="KPD58_003035"
     CDS             3495..4100
                     /gene="hisH"
                     /locus_tag="KPD58_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HBG0266766.1"
                     /translation="MNIIVDYGLGNIDSVSRGFRKAGIETKISSDIDEIKQADSLILP
                     GVGAFRDSISALDKLGLIPIIKEHVSKGKFMIGICLGMQLLYEKSYEYGEYEGLGLIK
                     GSIDKLDISLKVPHMGWNNLKFNKANDDILKYINEDDYVYFVHSYYANSSNEELIAFS
                     EYEKKIPAIVRKGNVYGIQFHPEKSGEVGLNILRAYGEMIK"
     gene            4097..4819
                     /gene="hisA"
                     /locus_tag="KPD58_003036"
     CDS             4097..4819
                     /gene="hisA"
                     /locus_tag="KPD58_003036"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HBG0266767.1"
                     /translation="MIIFPAIDIKDNKCVRLTQGEFDKVNVYYDNPLEVAYKWKNEGA
                     EYIHIVDLNGARSEFGVNTKIIEDIANNIDIPIQVGGGVRDKEKVKSLINAGVTRVIL
                     GSIAIENLNLVEELVNEYKEKIVVSIDAKDGKVAVRGWEVVSNVDSLTLCKQLEKIGV
                     QTIVYTDISKDGMLQGPNFDIYERIAKETSLNVIASGGVTSIEDVKRLKAMNLYGAII
                     GKALYDKKIDFKEAQQLCLLGE"
     gene            4801..5559
                     /gene="hisF"
                     /locus_tag="KPD58_003037"
     CDS             4801..5559
                     /gene="hisF"
                     /locus_tag="KPD58_003037"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HBG0266768.1"
                     /translation="MLTRRIIPCLDVRNGRVVKGKKFKDIVDVDSPEVLGKFYSDCGA
                     DELVFYDITASNEERKTSLEFVTKVAENINIPFCVGGGVNKLEDFTDILRKGADKVSI
                     NSSAVKNPELIREASLKFGAQCVVLSIDAKKNEEGSWSVYVKGGREKTNLDAIEWAVK
                     GVELGAGEIVVNSMDEDGMKNGYDIELLSKITSLVNVPVIASGGAGKKEDFYEAVNKS
                     NVDGILAASVFHFGEIKINDLKKYLKDMGVEVRL"
     gene            5587..6255
                     /locus_tag="KPD58_003038"
     CDS             5587..6255
                     /locus_tag="KPD58_003038"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HBG0266769.1"
                     /translation="MDNKCNNVYSDEVEKFIRSIKFDDKGLVPVVVQEVVSKDVLMLA
                     YMNKDAIKKTLKDKVACYFSRSRQELWVKGETSGNTQKVVKMSYDCDVDTILLFVEQT
                     GVACHTGNYSCFYRDLFDDSSKMELEVQTNILKELYDLINERKNNPVEGSYTNYLFEK
                     GIDKILKKVGEESSEVIIASKNTDKSELIYEISDLVYHTLVLMIEKGVEIDEIKKELL
                     KRRK"
BASE COUNT         2585 a          537 c         1251 g         1967 t
ORIGIN      
        1 aatatagtaa aattaaatac aaaattaaat catcaccatc atcatagatt aaagggattg
       61 taatatgaaa ttaattcata gacacaagcc ctgtttttag tgtaattggt cttgtatcta
      121 tgatgggaag taattttaag agaaaattat agccatatag gtataaagcc tatgtggctt
      181 ttttattgtt ttaaatttgc aaatataaga gtaaatttaa tgaagagggt gagaatgtga
      241 aatttttgag gattgaagat gaggttattt attctaaaaa aagatataaa ttaaaaaggg
      301 aaatagataa actttttttg gatgaagaat attttcaaat agaaccacaa ttatttgaag
      361 aatatgatga atttacaact ataaataata aaattccaga agaatctatg gtaaaggttg
      421 taaatggaaa agtaatggta cttagagctg atataactac aagtataata aaaagccttg
      481 taccaagatg ggaagatggt ttaaagctta agctttttta taattcatca atatataaaa
      541 ataaaaatac agttggaata aaagagataa gacaaatagg ttgtgagtat ctaggagaag
      601 cctctgtaga agcagataga gaagtagtga aattagctct taagatactt gaaaaataca
      661 ataataattt tattctagag gttgggagta gcaagtatat tcatggtttg ttagaagaat
      721 taaatttaaa taagaattgt gaaaatcaaa ttaaaaattt actatatacg aagaacactc
      781 atgaactaaa agtatatatt gaagaattaa aaattaagcg tgaagtaaaa gagttattat
      841 caaatatttt aagtttacaa gggaatttat ataatgttat tgaaaaatca aacaagttct
      901 attgtaataa taagatgaag caagctcttg aagaattaaa gcaagtaaat aatttgattg
      961 aggaatgtaa ttttttagat aaggctagat ttgatttatc aatgattact atgcttgact
     1021 attatgaagg aattatgttt agaggatact acccaaactc atataaagaa attcttagtg
     1081 gtggaagata tgattcttta actaaagagt atggaaaaga aattccagcc attggattta
     1141 cactaagtgt tgatgagtta atgaagtatg tatataagta aagtttagaa ggagggattg
     1201 ctttggatta tttgactata gcactagcaa aaggacgaat tgaaggggaa tcatttaaaa
     1261 aatttaaaaa aatgggtcta ggagacagta tagacacaga tacgagaaaa ctaattttta
     1321 aagatgaaga aaataaaatt atatatatac atgtaaagcc atcagatgta gttacatatg
     1381 ttgaaaaagg agtcgctgac ctcggtatag caggtaaaga tactatttta gagaatgaga
     1441 cagatgtata tgaaatatat gatttaggtt ttggaaaatg taaatttgct gtagcaggat
     1501 taaagggaga tagcatatat agagaagatg aatatttaaa agtggcaaca aagtatccca
     1561 atatagcaaa aaagtatttt aaagagaaag gtcaaaaaat tgaaataata aaattaaatg
     1621 gttctgtaga actagctcca atagtagggt tgtcagatgt aattgtagat atagttgaga
     1681 ctggcaatac acttaaagca aatggtcttg aaatattaga agacatatgt aatataagtg
     1741 ctagaattat atcaaataga gctagctata gatttaaata tgaacaaatt cagaatataa
     1801 ttcgtttatt tgaagaactt gataattaaa atataattga gctttataag gagggattta
     1861 agttgagaga aaaagaaagc ataagagagt taaagggata tgaaccaaat cacgtaaact
     1921 gtaaagtaaa acttgatgca aatgaaggaa gtaagaggtt gtttaaatac cttattaaag
     1981 aaatttcaga tagtgatata gatttaaatt tatatcctga agattcttat agtgatttga
     2041 aagaatctat aatagattac ataaatattt ctggagtaaa taagaaaaat cttctagtag
     2101 gaaacggttc aagtgaaata attgatttaa taatacatac ttttgtagat aaagatgaag
     2161 taatattgag tttctctcca agttttagta tgtattctat atatagtcag ataaatgggt
     2221 ctaaatttat aggtgttgaa agtgatgaaa atttagttat aaatatagat aatgtgattg
     2281 aaaaagtaaa ggagaataat cctaaaatag taattgtatg taatcctaat aatccaactg
     2341 gaacaatatt aaagagggaa gagataataa agcttttaga ttcaactaat agtctagtgg
     2401 ttttagatga agcatatatg gattttggag aagaaagcat gttaagtgat gtttttaagt
     2461 atgataatct aatagttctt agaactttat caaaggcatt tggattggct ggaataagaa
     2521 caggatatat gttatctaat agtagtttaa taaattctgt tgagaaagta agacctccat
     2581 ataatttaaa ttcattatca gactttattg ctacaagagc acttagaaat aaagatgtgg
     2641 taaaggctta tataaaggaa gtaaaagagg aaagagaagt tttatataaa gaaatgattg
     2701 gtatgggaat taaagcatat aaatcacaag caaattttat attattttat tctgaaatcg
     2761 aaaatttatc acaaaaactt attgatagag gtgttttaat aagaaaattt ggtggaaagt
     2821 tagaaaatta ttacagagtt acaattggtg ataaagaaga aaattctatg tttgttggag
     2881 ctataagaga tattttgaaa aaggagaaat aggtatgaga atttggaagg tagaaagaaa
     2941 tactcttgaa acacagattt tagtagaatt aaatattgat ggttctggta aagctgaaat
     3001 agatactgga ataggatttt tagaccatat gcttacctta atgtcatttc atggcaagtt
     3061 cgatttaaaa gtgatttgta agggtgatac atatgtagat gaccaccata gtgtagaaga
     3121 tataggtata gctataggtg aagcatttaa aaatgctcta ggtgacaaaa aaggaatacg
     3181 aagatattct aatatctata tacctatgga tgaatctcta tcaatggtag caatagatat
     3241 aagcaataga ccttatcttg tatttaatgc taaatttgat acccaaatga ttggtagtat
     3301 gagtactcaa tgctttaaag aattttttag agcttttgta aatgaatcga gagtaacatt
     3361 acatatcaat ctcttatatg gggaaaatga ccatcataag atagaaagta tttttaaagc
     3421 atttgcaaga gctttaaaag agggtagtga aatagtttct aatgagatag catcttcaaa
     3481 gggggttttg tagaatgaat ataatagtag actatggtct tggaaatata gattctgtgt
     3541 ctaggggttt tagaaaagct ggaatagaaa ctaaaatttc tagtgatata gatgaaataa
     3601 aacaggcaga ttcacttata cttccaggtg ttggagcttt tagagattct ataagtgcct
     3661 tagataaact aggattgata ccaataataa aggagcatgt ttctaagggt aaatttatga
     3721 taggtatatg tctaggaatg caattacttt atgaaaaaag ttatgagtat ggagaatatg
     3781 aaggattagg acttataaaa ggaagtattg ataaactgga tataagttta aaagtgccac
     3841 atatgggatg gaataatcta aaatttaata aggcaaatga tgatatactt aaatatataa
     3901 atgaagatga ttatgtatac ttcgttcact catattatgc aaattcatca aatgaagaac
     3961 taattgcatt ttcagaatat gaaaagaaaa tccctgctat tgttagaaaa ggcaatgtat
     4021 atggtataca atttcatcct gaaaaaagtg gagaagtagg gttaaatata ttaagagcat
     4081 atggggagat gataaaatga taatatttcc agcaatagat ataaaagata ataagtgtgt
     4141 aagactcaca caaggagaat ttgacaaagt aaatgtatat tatgataatc cattagaagt
     4201 agcatataaa tggaaaaatg aaggagcaga atatatacat atagtagatt taaatggtgc
     4261 tagaagtgaa tttggtgtaa atacaaaaat aattgaggac atagcaaaca atatagatat
     4321 acctattcaa gttggcggtg gagttaggga taaggaaaaa gtcaaaagtt taataaatgc
     4381 tggtgttact agagtaatat taggcagtat agcgattgaa aatttgaatt tagttgaaga
     4441 attggtcaat gaatataaag agaaaattgt agtttctata gatgctaaag atggaaaagt
     4501 agcagttagg ggatgggaag ttgtaagcaa tgtagactca ttaacattat gtaaacaact
     4561 tgaaaagata ggtgtacaaa ctattgttta tacagatata tcaaaagatg ggatgttgca
     4621 aggtccaaat tttgacatat atgaaagaat agcaaaggaa acatcactaa atgttattgc
     4681 ttctggaggc gtaacttcta tagaagacgt aaaaagacta aaagctatga acttatatgg
     4741 tgcaataata ggtaaagcgc tttatgataa aaagatagac tttaaggagg cacaacaatt
     4801 atgcttacta ggagaataat accttgtcta gatgtgagaa atggaagagt tgtaaagggg
     4861 aaaaaattta aagatatagt agatgttgat agtccagaag tacttggaaa attttatagt
     4921 gattgtggtg cagatgaact tgtattctat gatataactg catcaaatga agagagaaag
     4981 acatctttag aatttgtgac aaaagttgca gaaaatataa atataccatt ttgtgttggt
     5041 ggaggagtaa ataaattaga agatttcact gatattttaa gaaaaggtgc tgataaagtt
     5101 tctataaatt catcagcagt caaaaatcct gaattaataa gggaagcttc attaaaattt
     5161 ggagcacaat gtgttgtttt atcgatagat gcaaagaaaa atgaagaggg ttcatggagc
     5221 gtatacgtaa aaggtggaag agaaaaaact aatctggatg caattgaatg ggctgttaaa
     5281 ggtgtagaac ttggagcagg agaaatcgtt gtaaatagta tggatgaaga tggaatgaaa
     5341 aatggatatg atatagaatt attatcaaag ataacttctt tagtaaatgt acctgttata
     5401 gcatctggag gcgctggaaa aaaggaagat ttttatgagg cggttaataa atccaatgta
     5461 gatggaattt tagcagcatc tgtttttcat tttggcgaga taaaaataaa cgatttaaag
     5521 aaatatttaa aagacatggg tgtagaagta agattataag ttaaatacta aaaggagatt
     5581 agaaatatgg ataataagtg taacaatgta tacagtgatg aagtagagaa atttataaga
     5641 agtataaagt ttgatgataa agggttagtc cctgttgttg ttcaagaggt agttagcaaa
     5701 gatgtactga tgttagcata tatgaataaa gatgctataa agaaaacctt aaaagataaa
     5761 gtagcttgct attttagtag aagtagacaa gaactttggg ttaaaggtga gacatctggt
     5821 aatactcaaa aagtagttaa gatgtcttat gattgtgatg tagatactat actacttttt
     5881 gttgaacaaa ctggtgtagc ttgtcataca ggcaattatt cttgttttta cagagacttg
     5941 tttgatgata gttcaaaaat ggagcttgaa gtacaaacaa atatattgaa agaattatat
     6001 gatttaatca atgaaagaaa aaataatcca gttgaaggtt cttatactaa ttatcttttt
     6061 gaaaaaggaa tagataagat tcttaaaaaa gtaggtgaag agagtagtga ggtaattata
     6121 gcatcaaaaa ataccgataa aagtgaactt atttacgaaa ttagcgattt agtatatcat
     6181 acattggttt taatgataga aaaaggtgtt gaaattgatg aaataaaaaa agaattactt
     6241 aaaagaagaa aatagaataa gatagattaa tgggtaataa gaaaatatac cgcttttaat
     6301 aaaaagtggt atatttttta ttttatattt tgatttatag
//
LOCUS       DAELLV010000207         6272 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.207, whole genome shotgun sequence.
ACCESSION   DAELLV010000207 DAELLV010000000
VERSION     DAELLV010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6272
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.207"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..508)
                     /locus_tag="KPD58_003039"
     CDS             complement(<1..508)
                     /locus_tag="KPD58_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG0266770.1"
                     /translation="MIIISKENIKYYLIVVFIGILFFKFINTPSDFISSIEGFLKFFS
                     PFLLAILLALLLNPLVMLFEVKFKAHRLLAIFLSYIFIGFILAFAIRLLIPSIANTLN
                     RLINEMPMYTDYIDSFIEKNMSNIDFLKTLIPHIQHSLDNLLKEASNFVGKIPKNFLI
                     YTLSISSML"
     gene            699..1415
                     /locus_tag="KPD58_003040"
     CDS             699..1415
                     /locus_tag="KPD58_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBG0266771.1"
                     /translation="MRINKYIASCGIASRRKAEEIILGGRIKVNGSVVKELSFNIDEE
                     KDIVEFDNKKVKPSENYIYIVLNKPEGYITTVKDQFNRPSVIDILKDVRERVYPIGRL
                     DYETSGLLILTNDGDLTYKLTHPKHEIDKTYVASVKGIISDDEIRQFETGLKIEDYTT
                     APAKIRVTKENKEKNYSVCEITIHEGRNRQVRKMCKAINHPVLNLRRISVGKIVLKDT
                     KVGEYRYLTEDEIKYLKSIK"
     gene            1473..2051
                     /locus_tag="KPD58_003041"
     CDS             1473..2051
                     /locus_tag="KPD58_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG0266772.1"
                     /translation="MAYAKYLTKITDINKFYLENIINEGDIVIDATMGNGYDTRYLAE
                     KVGEKGFVYAFDIQEEAIKSTRKKLEKEGYTDRVKLIFDGHEKMNQYIKEEVSCVLFN
                     LGYLPRAKHSIITKPDTTLKAIKLSLEMLKENGVISIAIYTGHDGGQEEKDCIYNLVN
                     NLNQDEFNVLESKFLNQVNNPPQLILIEKKKA"
     gene            2445..3317
                     /locus_tag="KPD58_003042"
     CDS             2445..3317
                     /locus_tag="KPD58_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG0266773.1"
                     /translation="MEETNEMKDSKHLISNIQSQYTRLSKGQKLIAQYILNNYDKVAF
                     MTACKLGETVGVSESTVVRFANALGYSGYPKLQAALQELIKNKLTTVQRVEMAHDYSD
                     DFAILNKVLKSDIDNIRSTLEEIDERAFKEASNKLLRARKIYILGMRSSFVVAQYLGF
                     YLDIILDNVHIIRMDMGDAFEQIVRINEEDVIVAISFPRYSKKSYQIVNYAKEKGAHV
                     ISLTDSLFAPVASLADNTLLVKSNMASFVDSLVPALSISNALAISVGMKEKEDIKQHF
                     DDLEQIWKRYSVYE"
     repeat_region   4148..5554
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=4148..4176
                     /rpt_unit_seq="gttttatattaactatatggaatgtaaat"
     gene            5723..6007
                     /locus_tag="KPD58_003043"
     CDS             5723..6007
                     /locus_tag="KPD58_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266774.1"
                     /translation="MLVYNKSFYPNDIFPRLDFSKIKKQLKLIDNDLSDFGSICIIEK
                     EHYTISVNSIGEINVYYDLEYENKVYRIVYEIEKLFKSQVGRFSISTYRN"
BASE COUNT         2561 a          585 c          969 g         2157 t
ORIGIN      
        1 atagcataga agatatacta agtgtataga ttaagaaatt tttaggaatt ttaccaacaa
       61 aattacttgc ttcttttaat aaattatcta aactatgttg tatatgagga attaaagttt
      121 ttagaaaatc tatgtttgac atattttttt ctataaaact atctatataa tcagtataca
      181 taggcatttc atttatcaat ctatttaatg tattagctat tgatggaatt aataacctaa
      241 tggcaaaggc taaaataaaa cctataaata tataagataa aaaaatggct aaaagtctat
      301 gtgctttgaa ttttacctca aaaagcatca caagtggatt taaaagtaaa gcaagtaaaa
      361 tagctaataa aaatgggcta aaaaacttta agaatccttc aatactagat ataaagtctg
      421 atggagtatt aataaattta aaaaataaaa ttccaataaa cacgactatt aaataatatt
      481 ttatattttc ctttgaaata ataatcactt ccttaaaata gtgtttagtc agctattaca
      541 tattaatagt atgtgaaaag aaattacaat tatttaggaa aattaaataa tttttttgct
      601 tgtttttaat taaggtctag tatagtataa taatgtagca aaattaacat tgataaaaat
      661 attaaacaca cataatttaa gataaggaag gattaagtat gagaattaat aagtatatag
      721 cttcttgtgg aatagcatca agaagaaaag cagaagaaat aattcttgga ggaagaataa
      781 aggtaaatgg aagtgtagta aaagagttat cttttaatat tgatgaagaa aaagacatag
      841 tagaatttga taataaaaaa gtaaaaccaa gtgaaaatta tatatatata gtattaaata
      901 aaccagaggg atatataact acagtaaaag accaattcaa tagacccagt gtaatagaca
      961 tacttaaaga tgtaagggaa agagtatatc caatagggag attagattat gaaactagtg
     1021 gattattaat acttactaat gatggcgatt taacatataa gcttacccat ccaaaacatg
     1081 aaattgataa aacgtatgtg gcaagtgtga agggaattat aagtgatgat gagataagac
     1141 aattcgaaac aggactaaaa atagaagatt acacaacggc acctgctaaa ataagggtaa
     1201 ctaaagaaaa taaagaaaaa aattattcag tgtgtgaaat aactatacat gagggtagaa
     1261 atagacaagt aagaaaaatg tgcaaagcta taaatcatcc agttttaaat ttaagaagga
     1321 tttctgtggg gaaaatagta ttaaaagaca ccaaagttgg agaatataga tacctaacag
     1381 aagatgaaat aaaatattta aaatcaataa aataagataa aaataaattg agaaaaaatg
     1441 aaataaagtt aatagtaggg agaaatagta ttatggcata tgcaaagtat ttaactaaaa
     1501 ttacagatat aaataaattt tatttagaaa atattataaa tgaaggtgac atagtaattg
     1561 atgctactat gggaaatggg tatgatacaa ggtatctagc tgagaaggtt ggagaaaagg
     1621 gctttgtata tgcttttgat atacaagaag aagcaataaa gtctacaaga aagaaactag
     1681 agaaagaagg atatacagat agagtaaaat tgatatttga tgggcatgaa aaaatgaatc
     1741 aatatataaa agaagaagtt tcatgtgttt tatttaattt gggttatctt ccaagagcga
     1801 aacattcaat tataaccaaa ccagatacaa ctttaaaggc tataaagtta agtttagaaa
     1861 tgttaaaaga aaatggagta ataagcattg ccatatatac agggcatgat ggaggtcaag
     1921 aagaaaaaga ttgcatatat aaccttgtca ataatttaaa tcaagatgaa tttaatgttt
     1981 tagaatctaa gtttttaaat caagtcaata acccaccaca attgatttta attgaaaaga
     2041 aaaaagcata ataaatacta aaaaacttga caagattgta ttaagagtta atatatatag
     2101 tgtgacctat taaaaatact ttaaataata agtatacaac agcttattct caaaacaaaa
     2161 tatacatcta aatatgcaaa ataagataga taacttttaa atgagtaaaa attgaaaaat
     2221 ttaaaagaaa tattggataa atattaaaaa atgcaaggaa agatggtata ataaatttaa
     2281 tcgaaaatac tatgtgaaat ataaggtaca gaaaagttag aattttatga gtttaagaag
     2341 ctatgataaa aactaagatg aaattgagat aaaaaataaa atttttagag aaaaagacag
     2401 tgttaataaa acaaaatagt ttatataact aaaaggagaa aaaaatggaa gaaacaaatg
     2461 aaatgaagga cagtaaacat ttgatatcaa atatacaatc tcaatatacg agattgagta
     2521 agggtcaaaa acttatagca caatacattt taaataatta tgataaagtt gcatttatga
     2581 ctgcatgtaa attgggtgaa actgtaggag taagtgagtc tacagttgta agatttgcaa
     2641 atgcattagg ttatagtgga tatcctaaat tacaagctgc acttcaagaa ctcataaaaa
     2701 ataaactaac tacagtacaa agagttgaaa tggcacatga ttattccgat gattttgcaa
     2761 tactaaacaa agttttaaaa agtgatatag ataatataag gtctacacta gaagaaattg
     2821 acgagagagc gtttaaagaa gcttctaaca aacttttaag agcgagaaaa atatatatac
     2881 ttggaatgag gagttctttt gtagtagctc aatatttagg tttttactta gatataatac
     2941 ttgacaatgt tcatataatt agaatggata tgggagatgc ttttgagcag attgtaagaa
     3001 taaatgaaga agatgtaata gttgctatta gtttccctcg ttattctaag aaatcttatc
     3061 aaattgtcaa ttatgctaag gaaaaaggag cccatgtaat atcattaaca gatagcttat
     3121 ttgcgccagt agcatcactt gcagataaca ctcttctagt taagagtaat atggcttcat
     3181 ttgtagattc tctagttcca gctcttagta tttcaaatgc ccttgctata tctgttggga
     3241 tgaaggaaaa agaagatatt aaacagcatt ttgatgattt agagcaaatt tggaaaagat
     3301 attctgtgta tgagtaaagt ttgtatatta ataagtatta ttaattatta aaatgttcaa
     3361 atatagtgat gtttggataa aatatataaa atagctgact ggcatttaac tgacagtcag
     3421 tttttttatt taaacaatat tttgcttaaa ttttaaggaa tatctttgtc tatttcttct
     3481 aaatgagagt atttattcat agttattttc atttattata tttcttgttg attatatatt
     3541 tttttataga aaagaaaata gttatggttt tgtatctgtt atttttgcac ttattcaatt
     3601 tatttttgtg aaacctggaa agataaaact ttaatagcag ataataacct tgagattgta
     3661 aagtaacaaa gtgaaaatgt ataaagagga aaagaaatgg aaggaagaaa agtatataga
     3721 taaagagata taacacgtat tttgatttaa ctgtataaga tgaaaaattt gatgattttg
     3781 aataatttaa aaaattgtta atgaaaataa aagacttatt gagctaaggt gcgattacat
     3841 aggaaggatg caataaaaag aaaaaggaat cattggaata gagaatataa taaatactta
     3901 taagaatgta gatgctttta gtttgtaaaa ttatcccatt tttattttat agtatgagtt
     3961 ttatgatata ataaaaatat aaaagttttg cagtgagcga tttttgtgat aaagtagggt
     4021 ttaatagttg aaatataaag cgttgagagt gtatgataac tgttatcaat tgcactattg
     4081 ctcgctcact gcaagtttag gagaattgta tatgtataag tattggaaat acttaattta
     4141 ttttggggtt ttatattaac tatatggaat gtaaattttc tttttgaagt tgttgacgct
     4201 ctttttcatc agcgttttat attaactata tggaatgtaa atacatcaag actatctata
     4261 ttattaagag taactttagt tttatattaa ctatatggaa tgtaaatcta acttgtttcc
     4321 ctctaaagcg tgtttagtag ggttttatat taactatatg gaatgtaaat ctcttttgcg
     4381 cagagtggtt ttttacttgt ctaaatagtt ttatattaac tatatggaat gtaaattaaa
     4441 gacctttttt catgttgcac tctaaaaatc taagttttat attaactata tggaatgtaa
     4501 atgataagga atctgaatat taataacacc agaatctccg ttttatatta actatatgga
     4561 atgtaaatct actagcagga acatcaaaag gaggtttaaa atccgtttta tattaactat
     4621 atggaatgta aataccaaaa ggaatgttta tttttgactt tgcttatcaa ttgttttata
     4681 ttaactatat ggaatgtaaa tatacttggt caatacattt caaaagttgc aacagaacgt
     4741 tttatattaa ctatatggaa tgtaaataac ttatcagcat cttttttata agttccaacc
     4801 acgttttata ttaactatat ggaatgtaaa tttatcattt tttccgcttc tgcttgtaat
     4861 ttagactcgt tttatattaa ctatatggaa tgtaaatttc cctaatgctc cattttcatt
     4921 ataatctggt gtagttttat attaactata tggaatgtaa atgtaggtgt cggagagcta
     4981 ggaaactata gcataatggt tttatattaa ctatatggaa tgtaaatcgt tcttggaatg
     5041 ctaaaactcg tccttcttgg aataagtttt atattaacta tatggaatgt aaatctccca
     5101 ggagtaccgc tataaaaata aatcatacaa tgttttatat taactatatg gaatgtaaat
     5161 aatttttgaa atgcactgaa tactcattta ttacatcgtt ttatattaac tatatggaat
     5221 gtaaatagga acaaattgct aaaaaattag gggttggtta ttcgttttat attaactata
     5281 tggaatgtaa atctgagaaa gttttattct tttttaatgt tacaaatcgt tttatattaa
     5341 ctatatggaa tgtaaataaa ttattcccta aaaaaagttt atttctttct actagtttta
     5401 tattaactat atggaatgta aatattacag tttctttatt ttttattatt gtacttggcg
     5461 ttttatatta actatatgga atgtaaatac aatacccccc tattgcaatg cttcattaac
     5521 tattagtttt atattaacta tatgtaatat aaatgttttt tcttattaaa taaagattga
     5581 gtcactttat atttaataat acatattcta aattgatttt gtttttatat aagagaattt
     5641 gaataatatg gaaaaagatg attaataaat tattaattag ggtataaaat gatataaata
     5701 gaataaataa ggggtggatg aaatgcttgt atataataaa agtttttatc ctaatgacat
     5761 atttccaaga ttagattttt caaaaataaa aaaacagtta aaattgatag ataatgacct
     5821 gtcagatttt ggaagcatat gtataataga aaaagaacat tatacaataa gtgtaaacag
     5881 tataggtgaa ataaatgtgt attatgattt agagtacgaa aataaggtgt atagaatagt
     5941 ttatgagatt gaaaagttat ttaaatctca agttggaagg tttagtatat ctacatacag
     6001 aaattgataa ttaaaaaagt agaaattaaa aaacttaata ctaaagatat agataaatat
     6061 atgaggtaac agctagagag agttgttacc ctttttaaat actaagtata tattattctt
     6121 attaattttt tgtaaattca ctaaaagacc tttttataga atattatttt ctttttttat
     6181 taaataggta aatcagtaaa aatgcaattc cagttccaat tattattcca atgatatttt
     6241 ctataattaa tttttttaat ataaaagtaa at
//
LOCUS       DAELLV010000208         6181 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.208, whole genome shotgun sequence.
ACCESSION   DAELLV010000208 DAELLV010000000
VERSION     DAELLV010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6181
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.208"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..471)
                     /locus_tag="KPD58_003044"
     CDS             complement(<1..471)
                     /locus_tag="KPD58_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266775.1"
                     /translation="MNNKEIKRSLQKYKVPKYDSSALEKTIKTIHELTLSNTYKRMNN
                     KDFFISQIGFIRKRTWIMQFIILCTICIFLFSTSADSNSFYNTLSLISIASPLFVLTN
                     IEELTKVYNSSILEIEFSTKNTLHKIIATRMLIFGISDLLFMIIILVSAKQIIDV"
     gene            complement(468..1001)
                     /locus_tag="KPD58_003045"
     CDS             complement(468..1001)
                     /locus_tag="KPD58_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HBG0266776.1"
                     /translation="MTEVIIVNDARIISQIKAGKKELFDTLIKKYYDSIYCYCYRHVD
                     NKMTAQDLTQDVFVTVINNIENYKHYGKFENYLYVIAKNKCKDFWKKKQPIYLESLDK
                     EISNQELSDIENKMFIKEALTKLPELEFEVIILRYYHDLKFKDIAKIMESSVSVTKYR
                     MSRALKKLELYLEGGKL"
     gene            complement(1352..1873)
                     /locus_tag="KPD58_003046"
     CDS             complement(1352..1873)
                     /locus_tag="KPD58_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0266777.1"
                     /translation="MIPLLILGLLKQNPNSYGYELLALMEKKHYKYIVNFTKGSFYYN
                     LQQLEEKKFIEKVENKQSNKNRETHNYIITELGCQEFEKLMYKYGSKTEYINLSFYAT
                     LLFANDYKNEKLIELLETQIKQTQVRISLLNESIENDKTNSHYFNRMLENSRSHHLVN
                     IEWFEKLLNDIRD"
     gene            complement(1925..2773)
                     /locus_tag="KPD58_003047"
     CDS             complement(1925..2773)
                     /locus_tag="KPD58_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HBG0266778.1"
                     /translation="MIKSFLMLGQSNMAGRGFISEVTPIYNERIQMLRNGRWQMMTEP
                     INYDRPVSGVSLAASFADAWCCENQEDRIGLIPCAEGGSSLDEWNIDGILFKHAISEA
                     KFAIQSSELTGILWHQGENDSNNGNYKFYYKKLLSIIETLRKELNIPDIPIIIGGLGD
                     FLGKVGFGKSCTEYVFINQELQKFAFEQDNCYFVTATGLTSNPDGIHIDAISQRKFGL
                     RYFEAFHKKKHIMEALANESELIIPSNSKTYTKTEKIYINSMDLALGKISYDEFESKL
                     IKLNDN"
     gene            complement(2923..3204)
                     /locus_tag="KPD58_003048"
     CDS             complement(2923..3204)
                     /locus_tag="KPD58_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266779.1"
                     /translation="MPNIEKDLDGTTTITYLNDDIGVPTDENGYKISMTILHGKTICH
                     VVSPETILGRKFTLEEKQEKDTIIYGAIEKIMVDRQLQIKNSNKEVASH"
     gene            3589..4443
                     /locus_tag="KPD58_003049"
     CDS             3589..4443
                     /locus_tag="KPD58_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG0266780.1"
                     /translation="MEWIERLNKAINYIEENITKEIEYEQVAKIACCSTYHFQRMFAY
                     MADVPLSEYIRRRRMSLAAVELQNDDKKIIDVALKYGYSSPTAFNRAFKSIHGVAPSV
                     IKKREITTLKAFPPISFKISIKGAEEMNYRIEKKEAFRIVGVSQPLHNELEKNFEIVP
                     QMWQKVALDGTLQKLIPMMDSQLQGVLGISICNNSEEWKYFISVSSTKSIDNTLEEYT
                     VPAFTWAIFSGEGQCPQAIQELEKRIVTEWLPTSGYEYDNGPDIELYLNPDPQNAKFE
                     VWIPVVKK"
     gene            4642..6147
                     /locus_tag="KPD58_003050"
     CDS             4642..6147
                     /locus_tag="KPD58_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG0266781.1"
                     /translation="MNVAIYLRKSRADEEAEHRGEFETLSRHKTTLLKLAREQNLNVI
                     EIKEELVSGESIEYRPKMLELLGEIKNNNYDAVLVMDIDRLGRGNMQDQGLILDTFKK
                     SKTKIITPRKTYDLNDEFDEEYSEFEAFMARKELKLISRRMQRGKIKSIEEGKFIAQA
                     PPYGYDIKYLNNKERVLVINEDKATVVKIIFDMYLKGDGACKISNYLNELGYKTTTGR
                     TWYEKGIRDIIKNKVYCGYIVWNKVERKRNSSRTRGIEEQIEVKGKHEPIISEDIWNK
                     AQNIRKKRINSPTPNNKITNPLAGIVKCGYCNHTLIAKSNTTSNGYEKYMVCTNCKNK
                     ATKLYILENKILEVLNKWLVNYRLSLKDEKDNSKPENTLEHTLTNLNKELDTLEKQKE
                     RLHDFLEQGIYDNNTFGERLKILLSRIENVREAINKTNIDIEMQKERNTVKTQIVPQL
                     EKILSEYTEEKEPYLKNILLKSVINYAVYKKEPNQKSDDFSLILYPNIPRD"
BASE COUNT         2291 a          820 c          796 g         2274 t
ORIGIN      
        1 tacatcaatg atttgttttg cagataccaa aataataatc ataaacaata aatcacttat
       61 tccaaatata agcattctag tagcaataat tttatgtaat gtattttttg ttgaaaattc
      121 tatctctaaa atactactat tatatacttt tgttaattct tctatatttg tcagcacaaa
      181 aagtggtgat gctatagaaa tcaaggataa agtattataa aatgaattac tatctgctga
      241 tgtactaaat aaaaatatac atattgtaca tagtattata aattgcataa tccaagttct
      301 ttttcttata aaaccaattt gagatataaa aaagtcttta ttattcattc ttttataagt
      361 attactaagc gttaactcgt gtatagtttt aatagtcttt tcaagagctg aagaatcata
      421 tttgggaacc ttatacttct gtagacttct ttttatttct ttattattca taatttacct
      481 ccttccagat ataattctag tttttttaat gccctactca tcctgtactt tgtgacagag
      541 acgcttgact ccataatttt agcaatatcc ttaaatttta aatcatggta ataccgtaaa
      601 attatcactt caaattctaa ttctggtaat ttagtcaaag cttcttttat aaacatttta
      661 ttttctatat cagacaattc ttgatttgat atctccttat ctaatgactc taaatatatc
      721 ggttgttttt tcttccaaaa atccttacac ttgttttttg caataacata taaatagttt
      781 tcaaacttac cataatgttt ataattttct atattattta taactgttac aaatacatct
      841 tgagttaaat cttgtgcagt cattttattg tctacatggc gataacaata acaatagatg
      901 ctatcatagt atttttttat tagagtatca aacaattctt tttttcctgc ttttatttgt
      961 gatataatcc gagcatcatt cactattatc acctccgtca ttatatataa cgatttatac
     1021 tataaaaaag tcagtataaa aaaattaatt tattatttta taattattta taaatacagt
     1081 tatattaaaa ttatctatac cattaattaa tactcataat taatacttaa acaattttat
     1141 tatatcaaat gtcatacttt tcatcttaag tgtctgtaat tcaacaatga acaactttat
     1201 atttataaat tttacttaca tagtataagt gtcttttaac atcttatata acaaaaagac
     1261 agatattcaa ttgaaaatta tctgccttat ttttaatcta tatatcatac atttatataa
     1321 agtatatatg tatgtttttt tattattaaa atcaatctct aatatcattt agcaacttct
     1381 caaaccattc aatattaact aaatggtgag aacgagaatt ttctagcatt ctattaaaat
     1441 aatgtgaatt tgttttgtca ttttctatgg attcatttaa tagagaaatt cttacttgag
     1501 tttgcttaat ttgcgtttcc aataattcta ttaacttttc atttttataa tcatttgcaa
     1561 ataatagtgt tgcgtaaaat gatagattta tatactctgt tttagaacca tacttataca
     1621 ttaatttttc aaattcctgg catccaagtt cagtaattat gtagttatga gtttctctgt
     1681 ttttattaga ttgtttattt tcaacttttt caataaactt tttttcttca agttgctgta
     1741 aattataata aaaagaacct ttagtgaaat ttactatgta cttgtaatgc ttcttttcca
     1801 ttaatgctaa taactcatat ccataagaat ttggattttg ttttaataat cctaaaatta
     1861 ataatggtat caaaaaatca cctctttcaa tctatattta ttagtatatt aatgaatatt
     1921 ttatctaatt atcattaagt ttaataagtt tagattcaaa ctcatcatat gatatctttc
     1981 ctagtgccaa gtccatacta tttatatata ttttttctgt ttttgtatat gtcttggaat
     2041 tactcggtat tatcaactca ctttcattag ctaatgcttc cataatatgc tttttcttat
     2101 gaaaagcttc aaaataacgt aatccaaatt ttctttgaga tattgcatca atatgaatac
     2161 catcaggatt agatgtaaga cctgttgctg taacaaagta acaattatct tgttcaaaag
     2221 caaatttctg taactcttga ttgataaata catactcagt acaacttttt ccaaaaccta
     2281 cttttcctaa gaagtctccc aatccaccaa taataattgg tatatctgga atatttaatt
     2341 cttttctaag agtttcaata atagaaagta attttttata ataaaattta tagttgccat
     2401 tattactgtc attttctccc tgatgccata aaattcctgt caattcactg ctttgtatgg
     2461 caaatttagc ttcacttata gcatgtttaa aaagtatacc atctatattc cattcatcta
     2521 aggaactgcc tccctcagca caaggaatta gcccaattct atcctcttga ttttcacagc
     2581 accatgcatc tgcaaatgat gcagcaagac ttaccccaga aactggtctg tcataattaa
     2641 taggttctgt catcatttgc catcttccat tacgtaacat ctgtattctt tcattataaa
     2701 ttggagtcac ttcacttatg aatcctcttc cagccatatt ggactgtcct aacattaaaa
     2761 atgatttaat cattattatt attatccttt cttaaattct tattgtattt atttttaata
     2821 gtagtctaat tagactatct atgtttatat tatagtctaa ttagactata agtcaataat
     2881 attataaaaa atacttgtcc tactttactt attaaagaag tgctaatgac tagcaacttc
     2941 tttgtttgaa ttttttattt gtaattgtct gtctaccatt attttttcta ttgctccata
     3001 tattattgta tctttttctt gtttttcttc taatgtgaac tttcttccta agattgtttc
     3061 aggagatact acatggcaga ttgtctttcc atgtagtatt gtcatagata ttttatatcc
     3121 attctcgtct gttggaacac ctatatcgtc attgaggtat gttatagtag ttgtaccatc
     3181 taagtctttt tctatatttg gcatttaaat cccctcctct ttatatttta tgtatcttaa
     3241 aaattgtcct attctcaatc attatttata attgagtaac taagtgttgc ttgattaagg
     3301 gtaaacttca aaaatagtta actatgaata aaatgttata tataattaaa tgtctaagct
     3361 aaaatattat aaaaatttca taataaatta aaactgttat cttatataaa aattagatac
     3421 taatctgtga tatactttaa tgtgaaaaac aatactattt gttcattttt gtagttaatt
     3481 acagactttc tcatgttcat tctttattaa gctagttaat tagtgcatag aaagagagga
     3541 ttaactattt taaaatagat attatataaa ttacaaggag ctgattttat ggaatggatt
     3601 gaaagattaa acaaagcaat taactatatt gaagaaaaca taacaaaaga aattgagtat
     3661 gaacaagtgg caaaaatagc ttgttgttct acatatcatt ttcaaagaat gtttgcttat
     3721 atggcagatg taccactttc tgaatatatt cgtcgtagac ggatgtcttt agcagctgta
     3781 gaattacaaa atgatgataa aaagataatt gatgtggctt taaaatatgg atactcttct
     3841 ccaacagcat tcaaccgtgc atttaaaagt attcatggtg ttgccccatc agttataaaa
     3901 aaaagagaaa taacaacttt aaaagccttt cctccaatca gttttaaaat tagtattaaa
     3961 ggagccgaag aaatgaatta tagaattgaa aagaaagaag catttcgtat agttggtgta
     4021 tctcagccac ttcataatga actagaaaag aattttgaaa ttgttcccca aatgtggcaa
     4081 aaggttgctt tagatggaac gcttcaaaaa ttaatcccta tgatggatag tcaacttcaa
     4141 ggtgtattag gtataagtat ttgtaataat tcagaagaat ggaagtattt tatatctgtt
     4201 tcaagtacaa aatctataga taatacatta gaagaataca ctgttccagc ttttacttgg
     4261 gcaatatttt ctggagaagg acaatgtcca caagcaatac aagaactaga aaagcgtatt
     4321 gtaacagaat ggttacctac ttctggttat gaatatgata atggtcctga tattgaactg
     4381 tacttaaatc cagaccctca aaatgctaaa tttgaagtat ggatacctgt agtaaaaaaa
     4441 tagtttaaaa ttaaaaaatg aagaaagcat aaaattaata gtataaatat ttacttagat
     4501 tacaagcttt taaatatact ataaaattta ctgtataaaa ataaacctaa aaaatatatc
     4561 ttgtattttg aaggtttatt tttgtgtttt aaatataatc aatctgttac tgaatataat
     4621 atattcaagg ggtgtgattt aatgaatgtg gcaatttatt tacgcaaaag cagagctgat
     4681 gaagaagcag aacatagagg tgaatttgaa actctaagta gacataagac aactctttta
     4741 aaactagcaa gagaacaaaa tttaaatgtc attgaaatta aagaagaact tgtttctggg
     4801 gaaagtatag aatatagacc taagatgcta gaattactag gtgaaattaa aaataataac
     4861 tatgatgctg ttttagtgat ggatattgac cgtttgggtc gtggtaatat gcaagaccaa
     4921 gggttaatac tagatacatt taaaaaatct aaaacgaaaa ttattactcc tagaaaaacg
     4981 tatgatttaa atgatgagtt tgatgaggag tattctgaat ttgaagcttt tatggctcgt
     5041 aaggaattaa aattaatatc tagacgtatg caaagaggaa aaatcaaaag tattgaagag
     5101 ggtaaattta tagctcaagc tcctccttat gggtatgata ttaaatattt aaataataaa
     5161 gaaagagtac ttgtaataaa tgaagataaa gcaactgttg ttaaaataat ctttgatatg
     5221 tatttaaaag gagatggtgc atgtaaaata tccaattatt taaacgaact tggttataag
     5281 accacaacag gtagaacttg gtatgaaaag ggtataagag atattataaa aaacaaagtg
     5341 tattgtggat atatagtatg gaataaagtc gagaggaaaa gaaattctag tagaacaaga
     5401 ggaattgagg aacaaattga ggtaaaagga aagcatgaac caataataag tgaagacata
     5461 tggaataagg cacaaaatat aagaaaaaaa cgtattaatt cccctactcc aaacaataaa
     5521 ataacaaatc cattggctgg aattgtaaaa tgtggatact gtaatcatac tttaatagcc
     5581 aaatctaata ctacatcaaa tggatatgag aaatatatgg tttgtactaa ttgtaaaaat
     5641 aaagctacta aactatacat attagaaaat aaaatattag aggtcttaaa taaatggtta
     5701 gtaaattata gactatcttt aaaagatgaa aaagataact ctaaacctga aaatacttta
     5761 gaacatacct tgacaaattt aaataaagaa ttggatactt tagaaaaaca aaaagaacga
     5821 ctacatgatt ttttagaaca aggaatttat gacaataata catttgggga acgtttaaaa
     5881 atactcttat ctcgtataga aaatgtaaga gaagctatta ataaaacaaa tatagatatt
     5941 gaaatgcaaa aggaaagaaa tacagttaaa acacaaattg ttcctcaact tgaaaaaatt
     6001 ttaagtgaat atacagagga aaaagaacct tatctaaaaa atatattgct taaaagtgta
     6061 ataaattatg cagtctataa aaaagaacct aatcaaaaat ctgatgattt ttctttaata
     6121 ctttacccta atatcccaag agattaatta tatgttttaa atgttataaa tagactgatt
     6181 a
//
LOCUS       DAELLV010000209         6133 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.209, whole genome shotgun sequence.
ACCESSION   DAELLV010000209 DAELLV010000000
VERSION     DAELLV010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6133
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.209"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            213..1520
                     /locus_tag="KPD58_003051"
     CDS             213..1520
                     /locus_tag="KPD58_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266782.1"
                     /translation="MKEIADAITSEYLSYGINEDISIYEGRFCIYLDKKYRCNGKIYY
                     KMTPPISISFKADIGCVEEIDNEDDNLALDYDNAILEVHGYKIISITINTLSEFSVEG
                     YINDDCIKSKNSYVEYVDFNIINLDKIPGKLIKYNDKVYAGRIEFDINDYVVTIDKRY
                     DYRKELKSELKSKSGAIITHIGRIRRKDGRIFRTNNTINLLDRISTALSFMCGRYVGF
                     CLAKGYRSGNEVYRIWNENQISPFRYVPTWSDTLSNYYNMEKYISLMCKKLEDFYYGS
                     AIKSVVDWYIESLGSATMENNIISVQIALETLSYVILVEQNKILTDEVFDCNLASKNI
                     RLLLDTCKIPYGKHELNIFDNIIKNKFDDGVDLVIYLRNSIVHPSRKTHRAVLEVEDI
                     WNIISIGTRYIELVLLFILGYRGEYSNRLVERCYGEVEVVPWN"
     misc_binding    1606..1868
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1994..2797
                     /locus_tag="KPD58_003052"
     CDS             1994..2797
                     /locus_tag="KPD58_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG0266783.1"
                     /translation="MKNILKKVGIFTIMLGLLGGVVGCSKPDNEKDKDASKESKKEVV
                     VGFDNTFVPMGFLDEKGDTVGFDVDLAKETFKRLGMEVKFQPIDWSMKETELNDSKTV
                     DVLWNGYSITDERKKIVSYTEPYLQNKQIIVTLSDSKINSKADLKDKEVGTQQGSTAL
                     DAVEKDKDFMNSLKGGAPVLYDTYDKALRDLEIGRTSAVVGDEVLIRYYMGQKGEDKY
                     KVLKDDFGLEDYVVATSKENPELCEKINETLKEMKKDGTFDKIYDKWFK"
     gene            2835..3446
                     /locus_tag="KPD58_003053"
     CDS             2835..3446
                     /locus_tag="KPD58_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBG0266784.1"
                     /translation="MLQGLEIVIAMFCITLIVSIPLGIGVAFLRLSKNKLVSGITQCY
                     ILIMRGTPLLLQMIVIFYGLPLLGIVFDRFTAGVVAFFLNYAAYFAEIFRGGIQSIDR
                     GQYEASKVLGFDKFTMYKRVIFPQVFKRILAPISNEVITLVKDTSLVYILGLNDILRI
                     SQIAMNREASLLPLFEAGAIYLIFVAILTKGFELLEKKYSYYR"
     gene            3478..4149
                     /locus_tag="KPD58_003054"
     CDS             3478..4149
                     /locus_tag="KPD58_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBG0266785.1"
                     /translation="MLKIKNLNKSFKKNRVLKDISFELEEGQIGVLLGKSGAGKTTIL
                     RCINGLEEFDSGEIIIDNEVIKNKRDMAKIRGKIGMVFQNFNLFPHMTVLENIIESPV
                     NVFKVPRKEAEERARELLRLVDLEDKLNSYPFELSGGQQQRVAIARSCALMPKVLCFD
                     EPTSALDIDTIQRVVNIMNRLKDKGMTILIITHDVVFSNNVADKIISIKDGIVENVQI
                     KEKIV"
     gene            4447..4809
                     /locus_tag="KPD58_003055"
     CDS             4447..4809
                     /locus_tag="KPD58_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HBG0266786.1"
                     /translation="MIKLYGYTKCSTVKKAKNWLKENNLEFEDIDMVQNPPSKEELKS
                     IYKTSGYDIKKFFNTSGMKYRELGLKDIVKTESDDKLLEILVSDGMLIKRPLLLDGKN
                     VLLGFKEDVWKSTLLKED"
     gene            5245..5946
                     /locus_tag="KPD58_003056"
     CDS             5245..5946
                     /locus_tag="KPD58_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HBG0266787.1"
                     /translation="MSIEDLYDFECVPLNNEIDIDVLFEKMIEDGKEKEYTPIIVIDE
                     KVGLLRKNIDMITKEYGSLENYREYCLTKYNEIDVNQFFDFRKSEISELLKELIDLED
                     DFYDYKHFRPIMNIDIFHEYNKVYIAKIPTIKPYEVFAYIPIGGFNDCPRDEEHIAIA
                     KYWYEKHGIFPIAIGCDTVQYSVKNLTKDGKKFDDLCIELVFYCEDIIIQGYETLKAL
                     KDVLKRSTIVLFWWD"
BASE COUNT         2466 a          550 c         1057 g         2060 t
ORIGIN      
        1 caagctagtt atctaagttt tcggataact aaatctaaac ggaatacata atgtattaaa
       61 taaatgatga tactaaaatg atactatttt tatagaaatt tattgaggta tataattaat
      121 gaaaataata tattaaatta tataaattaa attatataaa ttaaattata taatatattg
      181 agttttacat aaaagcaaag gtgggaataa aaatgaaaga aattgctgat gctataacat
      241 cagagtattt aagctatggt atcaatgaag atattagtat ctatgaaggt agattttgca
      301 tatatttaga taaaaaatat agatgtaatg ggaaaattta ttataaaatg acacctccaa
      361 tatctataag ttttaaagca gacataggtt gtgttgaaga gatagacaat gaagatgaca
      421 atttagcttt agattatgat aatgctattc ttgaagttca tggatataaa attatttcta
      481 taacaataaa tactttaagt gaatttagtg tagaaggtta tatcaatgat gactgcataa
      541 aatctaagaa ctcctatgtt gaatatgtag attttaatat aataaactta gataaaatac
      601 ctggaaaact cattaaatac aatgataagg tatacgcagg tagaattgaa tttgatataa
      661 atgattatgt tgtaactata gacaagcgat atgattatag aaaagaatta aaatcagaat
      721 taaagtctaa atctggagca ataataactc atataggtag gattagaaga aaagatggta
      781 gaatttttag aacaaataat acaataaatc ttttagatag gatatcaaca gctttgagtt
      841 ttatgtgtgg aagatatgta gggttttgtt tggccaaagg gtatagaagt ggtaatgaag
      901 tttatagaat atggaatgaa aatcaaatat ctccttttag atatgtacca acatggtcag
      961 atacactttc aaattattat aatatggaaa aatatataag ccttatgtgt aaaaagctag
     1021 aagattttta ttatggttct gctataaaga gtgttgttga ttggtacata gaatctcttg
     1081 gaagtgctac catggaaaac aatataatat ctgtacaaat tgcacttgaa actttgtcat
     1141 atgtaattct agtggaacaa aataagatac ttacagatga agtatttgac tgtaatttgg
     1201 catctaaaaa tataagactc ttacttgata catgtaagat accatatggt aaacatgaat
     1261 taaatatatt tgataatata ataaaaaata aatttgatga tggagtggat ttagttattt
     1321 atttaagaaa tagcatagtt catccaagta gaaagactca tagagctgtg ttagaggttg
     1381 aagatatttg gaatataatt tcaataggaa cgagatatat tgaacttgtt ttactcttta
     1441 ttttaggtta tagaggagaa tattcaaaca gattagtaga aagatgttat ggtgaagtgg
     1501 aagttgtacc ttggaactag atttttatat ttattttaaa atatatattg acatatattg
     1561 ccttatgcta taaaatcaat atcataataa taattcagaa taacattaaa tgctaagaaa
     1621 agaaatagta aaagtagtta atcttaaaca gagagtttcc gattggtgag aggagcaata
     1681 gattttactt tgaacacatc tttgagcagg actctgaaag agtagaaata ctttattagg
     1741 agatttccgg aatccttgca cccgttatag tgctagagta taataatttt attgtactta
     1801 aagaggttag tgtcgtgagg cattaatgaa cagaggtgga accacgagag ttaactcccg
     1861 tcctctagca gaagctagag gcgggagttt tttaatataa ttttttaagc gcgcagagaa
     1921 ttatattgca atagatacac tttttaaaaa ataatcaaaa tattttaaaa attaaaaggc
     1981 gagggagaat attatgaaaa atatattaaa aaaagtagga atatttacaa ttatgttagg
     2041 tttattaggt ggagttgtag gttgttcaaa accagataat gaaaaagata aggatgcttc
     2101 aaaggaatct aaaaaagaag tagttgtagg gtttgataat acgtttgtac caatgggatt
     2161 tttagatgaa aagggagata cagtaggatt tgacgtagac ttagctaaag aaacatttaa
     2221 aagacttgga atggaagtta agtttcagcc tatagattgg tcaatgaaag aaactgaatt
     2281 aaatgattca aaaactgtag atgtattatg gaatggatat tctataacag acgaaagaaa
     2341 gaaaatagta tcatatacag aaccatattt acaaaataag cagataatag ttactctaag
     2401 tgactcaaaa ataaattcaa aagctgattt aaaagataaa gaagttggaa cacaacaagg
     2461 ttcaacagca cttgatgcag tggaaaagga taaagatttt atgaatagct taaaaggtgg
     2521 agcaccagtt ctttatgata cttatgataa agcacttaga gatttagaga taggtagaac
     2581 tagtgcagtt gttggtgatg aagttttaat tagatactat atgggacaaa agggtgaaga
     2641 caaatacaag gtattaaaag acgattttgg attagaagat tatgttgtag caacttctaa
     2701 agaaaatcca gaactttgtg agaaaataaa tgaaacatta aaagaaatga aaaaagatgg
     2761 aacttttgac aagatttatg ataagtggtt taaatagaag ataagttagc tgttaagaag
     2821 ggggcactaa aggcatgtta caaggtttag agatagttat agcaatgttt tgtataacat
     2881 tgatagtatc aattccattg gggattggag ttgcattttt aagattatca aagaataaat
     2941 tagtaagtgg tattacacaa tgttatatat tgataatgag aggtacacct ttacttttgc
     3001 aaatgatagt catattttac ggattacctc ttttaggaat tgtatttgac agatttacag
     3061 ctggagtagt agctttcttc ttaaattatg cagcatattt tgcagaaata tttagaggtg
     3121 gaatacagtc aatcgataga ggacaatatg aagcttcaaa agtactagga tttgataagt
     3181 ttactatgta taagagagta atctttccac aagtttttaa aagaatacta gcacctattt
     3241 caaatgaagt aatcacatta gtaaaagata catctttagt atatatatta ggattaaatg
     3301 atattttaag aatatctcaa atagcaatga acagggaagc aagtctgtta ccattatttg
     3361 aggcaggagc gatatatctt atatttgtag ctatacttac taagggattt gaattacttg
     3421 aaaaaaaata ttcttattat agataaagtt acaatattat aggaggaggt tttttatatg
     3481 ttgaaaatca aaaacttaaa taaatcattt aaaaaaaata gagtacttaa agatatttct
     3541 tttgagttag aagaaggtca gattggagtt ttacttggta agtctggtgc aggaaagacc
     3601 acaatattaa ggtgtataaa tggtcttgaa gaatttgata gtggagagat tataatagat
     3661 aatgaggtaa taaaaaataa gagagatatg gcaaaaatac gaggaaaaat aggtatggtt
     3721 tttcaaaatt tcaacttatt tcctcatatg acagtccttg aaaatataat tgaatcccca
     3781 gttaatgttt ttaaagttcc tagaaaagag gctgaagaga gggctagaga acttttaaga
     3841 ttagttgatt tagaagataa attaaattct tatccatttg aattgtcagg aggacagcaa
     3901 cagagagttg ctatagctag gtcatgtgca ttgatgccaa aagtcctttg ttttgatgaa
     3961 cctacttctg cacttgatat tgatactatt caaagagtag tcaatataat gaacagactt
     4021 aaagacaaag gtatgacaat attaataatt acacatgatg ttgtcttctc aaataatgtt
     4081 gcagataaaa ttataagtat aaaagatggt atagtagaaa atgttcaaat aaaagaaaaa
     4141 attgtttaga gttttataaa tataaactag ggtagaattt tataatataa ataaagggca
     4201 agtaaaatca aatatactat gcccttttgt tatctcaaaa tttaatctta tattttatgt
     4261 ttaagttaca ttataattta taataaaaat aaaaggacta tttaaatata taatgtgaat
     4321 aatgtttaat aatttgaaat atgataattt tttatgttga aagcttataa tgattatgat
     4381 ataaattggt tatagtatac aatttatggg tataacaata aaaaattaga aaggaatttt
     4441 tataaaatga ttaaattata tggatataca aaatgctcta ctgttaaaaa agctaaaaat
     4501 tggcttaagg aaaataattt agaatttgaa gatatagata tggttcaaaa ccctcctagt
     4561 aaagaggagt taaaatcaat ttataagact agtggatatg atataaagaa attttttaat
     4621 acaagtggta tgaaatatag agaattgggt cttaaagata ttgtaaaaac tgaatctgat
     4681 gataaacttt tagaaatatt ggtaagtgat ggtatgctta ttaaaagacc attacttttg
     4741 gatggtaaaa atgtattatt gggttttaaa gaagatgtat ggaaaagtac tttgctaaaa
     4801 gaagattagg aagagatagg tgtgttgatt aaaattatta attaatacac ctataaattt
     4861 atgtattaaa tctaatcttt ataaatatta attagcaaat tcaaatcaaa aaaattcata
     4921 tcaacaaaca tattacttct tattacaaat tatatcatat gttttatatt aaaatattat
     4981 aatattacaa cgaattgtta caaaattact tattattaac catgaacatt tgttttgtgc
     5041 tatcatattt atataaggga attgtcataa aaaatatctt gttcatacag ttttatatag
     5101 aaaaaagaaa tgaatctttt ctttatataa gtatattttg taaattgcct ctatatttat
     5161 agtttaaata atagtggtat taaaaatctg acaacaaaaa taaaactttc aaaaaattaa
     5221 atctaaaaag aataggagat aaatatgagt attgaagatt tatatgattt tgaatgtgtt
     5281 cctttgaaca atgagattga tattgatgtt ttatttgaaa agatgataga agatggaaaa
     5341 gaaaaagaat atacaccaat tatagtcatt gatgaaaaag tagggctatt aagaaaaaat
     5401 atagatatga ttactaaaga gtatggttct cttgaaaact atagagagta ttgtttaaca
     5461 aaatataatg aaattgatgt aaatcaattt tttgatttta ggaagagtga gatttcagaa
     5521 ttattaaagg agctaattga tttagaagat gatttttatg attataaaca ttttagaccc
     5581 attatgaata tagatatatt tcatgaatat aacaaagttt acatagctaa aattccaaca
     5641 attaaaccat atgaagtatt tgcatacata cctattggcg gatttaatga ttgtcctaga
     5701 gatgaagaac atatagctat tgcaaaatat tggtatgaaa agcatggaat ctttccaatc
     5761 gcaattggat gtgatacagt tcaatattct gtaaaaaatt taactaaaga tggtaaaaaa
     5821 tttgatgact tatgtattga attagtattt tattgtgagg atattattat acaagggtat
     5881 gaaaccttaa aggctcttaa ggatgtatta aaacgctcta caatagtgct tttttggtgg
     5941 gattgatatt ctatcattta gatagtatta aattgttagt caccaagaat ccacttgctt
     6001 tagttatagg gagtgtgtca aactaggttt ttatacacag tgtagcatag taatatgttt
     6061 aaattacaaa ggttaaaaaa atactttaat atttttaata ttataattaa tagatttaag
     6121 gtaattataa ttt
//
LOCUS       DAELLV010000210         6121 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.210, whole genome shotgun sequence.
ACCESSION   DAELLV010000210 DAELLV010000000
VERSION     DAELLV010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6121
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.210"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            166..786
                     /locus_tag="KPD58_003057"
     CDS             166..786
                     /locus_tag="KPD58_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBG0266788.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(952..1488)
                     /locus_tag="KPD58_003058"
     CDS             complement(952..1488)
                     /locus_tag="KPD58_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBG0266789.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(1601..2275)
                     /locus_tag="KPD58_003059"
     CDS             complement(1601..2275)
                     /locus_tag="KPD58_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBG0266790.1"
                     /translation="MSLFIISQCITSFAFTPENNKFKVKPLPYAYDALEPYIDKETMK
                     LHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVRNNAGGAYNHK
                     FFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWAWLIATKDGKL
                     SIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVVNWNGALENYK
                     NLKSQD"
     gene            2478..4352
                     /gene="asnB"
                     /locus_tag="KPD58_003060"
     CDS             2478..4352
                     /gene="asnB"
                     /locus_tag="KPD58_003060"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBG0266791.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLREAFREIVPEHVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            complement(4444..5880)
                     /locus_tag="KPD58_003061"
     CDS             complement(4444..5880)
                     /locus_tag="KPD58_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBG0266792.1"
                     /translation="MICTIKPLKISIKKSTEPLIEFISDTTYKNKSSSYIKEVYFTRY
                     IGVDDSIDKYIEKIKNIDTYFFNNPTIPYIRLNNLKINFDKEQTDKMLKIFNIYNSSK
                     FNSWDLYNIDFPCKIENDTLNWTKKIAFKNTLDLFSTSMPNCTSSIIKNFAVKLLCWI
                     DYFLPKLFYNKVKTTISPKIVYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDLYP
                     ECSKFSTLFELSRKTPNILEIPFERHITNINYSENTNKTSPTTNKPNSKNTLNNTNLT
                     SKKIVQDEEVELSYEDLAKLSASVVMIVVCNNENKPFKSGSGVVINNEGYILTNLHVV
                     NDGYSFLVRFENDDKVYTSYQIIKYHSDYDLAVIKVDRKCKPIPVKVSKKPVRGQKIV
                     AIGSPLGLFNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLISAG
                     YDDGQNLNLAVESSLVKIFANNFIEIVN"
BASE COUNT         2354 a          713 c          889 g         2165 t
ORIGIN      
        1 aacatcacct tttttgttat aatatataat aaaataatta taccttaact gtaaacttat
       61 atagattatt tactttttaa tattagattc attttttgag attggtatat aataaacata
      121 tagagtatat aataatagtt aaagttaaaa aaggggaaaa gcaatatgca gtatatacaa
      181 gaactacttg atttgactca aaattattgg gtgttagcca caataatcgg cttattaagt
      241 gcatttatag aaagctttat accagcctta ccacttgttg ctatagttac agcaaatgct
      301 gcaatacaag gattatttat tggatgtttg ttatcatgga ttggctcagg acttgggaca
      361 acttcattat tcttattaat aagcagattt aatgatagta agctttttaa taagttgaga
      421 aatagtaaaa ctgaaaaagc aataagttgg atggataaac agggatttaa gttattattt
      481 atagcgtatg cctgtccatt tatgcctggt tgtttagtta ctatcgcatc agcttttggt
      541 aaaaaagatt taaaagattt tgtgccagca atgttggcag gaaaatttgt aatgtttata
      601 gttataagtt acgtggcaag tgatatagaa ggttttataa caagtccttt aaagatagca
      661 tcatttatat tattggtgtt tttgtcatgg aagattggaa atagagtaaa taaaaatctt
      721 gaaaatcata attatgattt ccatcataaa aaacatcaca atgataaaga tgataaaatg
      781 atttagcttg tacgaaataa aacgtatctt aaaatgattt agtactaaat tattttaaga
      841 tacgttttat tttttgtatt aaggtacaaa atttttagtt cttttgtaaa ctaaaatata
      901 taaaacttat ttatacttag ttcaaaatat tgttatttat tttttctaca actataataa
      961 aatataaaat tgatatccaa tcaaaaaaat aactatgaaa tttaatagct tagatatgaa
     1021 tttttctgtt tttgagttag tttttatagg tagtaaagaa atgtttatgg taatgggata
     1081 aaacaattca ataccttctt tagtaataag gtcaagacac aagtgagata gatatcctat
     1141 tataaaccca ctggacaaac atgagaaaac tatattattg ttagtgaaga ttgtcagaga
     1201 tttaaaaaaa ctataaataa gtaataaggc aattagactg tgagtaaacc ctctatgtct
     1261 aaacctactt ccaaataatt tgtatattaa cataaatttt ttacttatgt aagaacctct
     1321 catatctata tctggaaata aagaacctaa atatgcaaaa taaatataaa ttataattaa
     1381 tactatttta tatgcaatat cgtatttaac taggtacaga ttgtttataa aggggagtat
     1441 caaaagtgcg aatatataac ctcccctttc atgtgtttct tttatcatat aaaaatcctc
     1501 ctgaatcgtc actataaaca ttatattggt aaatttagac tatcttttat tatattatat
     1561 agcaaacata agtttttttc aaaaggattt taatatttaa ttaatcttga gattttaaat
     1621 ttttgtaatt ttctaaagca ccattccaat ttactacatt aaaccagttg tcaatgtatt
     1681 catttcttct attttgatat tttaaatagt aagcatgctc ccaaacatca agtcctatta
     1741 taggagttag gtttttactt acagggctat cctgatttgg agtagtcata atagataatt
     1801 tgccatcttt agtagctata agccaagccc aaccagaacc aaagacatct aaagcagatt
     1861 tttggaactc ttgcttaaat ttttcaaaag aaccaaagtc tctatcaata gcttctttta
     1921 aagattcaga aggtatggtt ttttctggtg tcattatatc aaaaaagaat ttatgattat
     1981 aagctccacc tgcattattt cttacagttg tagcaatatc tttaggtaaa gaatctaaat
     2041 tttgcaataa ttcacataaa gaataattat aaagctcagg atatttttca agagcagcat
     2101 ttaatttatc aacataagct tgataatgct tatcatgatg cagtttcatt gtttctttat
     2161 ctatataagg ttcaagtgca tcatatgcat aaggtaatgg tttaacctta aatttgttat
     2221 tttcaggtgt aaaagcaaat gaagttatgc actgtgagat tataaataaa gacataataa
     2281 ctggtattaa tatttttttc ttcataaaaa tcacctccta atatagtatt tctagaaata
     2341 aaaaaaatat gaaatattaa cagataattt ataagaattt aatagcaatt tcattttaaa
     2401 ttgaataagt tttaaagcca aagacaaaac tatgaaatat agaataagac taaaggacag
     2461 aaaggaattt tgattatatg gacggcaata tttcaattta ttataaaggt gatatatcta
     2521 aaaaagaaga tattaaattt aaaataaaag aatctgtaaa taatacaaga ctttctagta
     2581 gaatagagta taaggatgag gaaaaaactt ttgattttga aagatttaat gtaattgatg
     2641 ctatttatgg aaataaacca tttgaaaaag atggaaaagt tattgttttt aatggaaata
     2701 tatataacta tcatgaaata aaagaagaat taattaaaaa aggacacaat tttactacaa
     2761 atgatgatat tgaagtctta ttagcatctt atatagaatt tggtaagaat tgtgtacata
     2821 agattaaagg aatgttcaac tttataatct atgatagaga aaatgaaagt atatttggag
     2881 caagagattt atttgggata aaaccacttt attatataaa taaggaaaat gcaataattt
     2941 tttcctcaga gtataagttt ttacttgaat atatgaaaaa tttaaatata aatgaaagaa
     3001 gcttacaaag ttatttttca tttcaatatg tacttcctga agatactatg atacaaggaa
     3061 taaggctgat accagcaggt cactatttta gggtagaaaa tggtatacta tctttaaaga
     3121 gatataataa acttgagttt agaagtagta caaaattttt ttatactaaa aatcacttag
     3181 gcaatagaga tgtgaatgag gatgatataa gaaatatagt agtagattca atagtaactc
     3241 atatggaaga ggagaaagaa attggaacat ttttgtctgg tggaatagat tcatctatta
     3301 taactactgt ggcttcacaa attaatccaa atataaaatc tttttctgca ggctttagtg
     3361 tcaaaggata tagtgaatta gaagtagcaa agaaaacagc tgataagtta ggtatagaaa
     3421 atatccagat taatataaca caagatgaat atataaaatc attaccgaat gtaatttatt
     3481 ctttggatga ccctatagca gacccttcag aagtaggtct ttatttctta attaaagagg
     3541 caggtaaaca tgttaaagta gcattgtctg gagaaggagc agatgaatta tttggaggat
     3601 ataatatata taaagagtat agtactatga aatcagttgt caattcacct acttatatta
     3661 aaagtatatt aggaagagtt tcagaactta tgccaaacat aaaaggaaga aattatcttt
     3721 atagagctac aacaccatta gaaaaaagat atataggaaa tgcaaaagtt tttgaaaaca
     3781 gcgaagtaaa aagattcttt tttaagtata aggaaaagaa tatatatgaa tacctgttgt
     3841 ctaatttgta tagagatgca caaaaaaata attacgatta tataagtaaa atgcaacata
     3901 tagatgtgaa tacatggctt caaggcgata tacttcaaaa aattagcaaa ctttctacag
     3961 ctgagcaggt tgaacttaga gtaccatttt tatataaaga tgtatttgat gtcgcaaaaa
     4021 atttaagaat ggagcaaaaa ataaataaga ataatacaaa agttttactt agagaggcgt
     4081 ttagagaaat agtcccagaa catgttgttc aaaggaaaaa attaggattt ccaactccaa
     4141 taagagtttg gttaaaagat tctctagggg atattgtaaa ggaaactatt tataattcaa
     4201 atgtagatga atttatagat aaaaaatatg caataaaact tttagatacg catcttaaag
     4261 gtcatagaga taattcgaga aaaatatgga ctatatttac attttgtcta tggcatcaat
     4321 tatttattga gcataaaaat gtcgagtatt aatacttttt tcaatattaa tcagagggca
     4381 aaagcatgca atgttttcac cctcttgatt attatggaat aggataagta gaattttgat
     4441 tgattagttt actatctcaa taaaattatt ggcaaatatt ttgacaagac tactttcaac
     4501 agctaaattt aagttttgtc catcatcata tccagcggaa atcaatccta gtaagtttcc
     4561 aaacatatct aaaagagctc ctcctgaact accactagag attggagctg taaattgtat
     4621 catttgaacc gtttcaaaat cacgaaaagc tgaaataatt ccatctgaaa ctgtattaaa
     4681 aagacctaga gggcttccaa ttgcaactat tttttgacct ctcacaggtt ttttactgac
     4741 tttaacaggt attggcttgc attttctatc aacctttatc acagctaaat cataatctga
     4801 atggtatttt ataatttggt aagatgtata aactttatca tcattttcaa atctaactaa
     4861 aaatgaatac ccatcattga ctacatgaag attcgtaagt atataacctt cattatttat
     4921 tactactcca gaaccgctct taaacggttt attttcgtta ttgcacacaa caatcataac
     4981 aacagaagct gataactttg caaggtcttc ataagaaagt tctacttcct catcttgtac
     5041 aattttttta gatgttagat ttgtgttatt aagtgtattt ttagagttag gtttattcgt
     5101 agtaggagaa gtcttattgg tattttcact gtaatttata tttgtaatat gcctttcaaa
     5161 aggtatttct aatatatttg gagtcttcct agaaagttca aatagagtag aaaattttga
     5221 acattcagga tataaatcaa gaatatcttt atttggattt atatataaaa tatcacaccc
     5281 aagttgtgat aaaaaatata agaaaaaaag ttcttgcctt ttaatatttc caaagtatac
     5341 tatctttggt gaaatagtag tcttcacctt gttataaaat aatttaggta gaaaatagtc
     5401 tatccaacac aaaagtttaa ctgcaaagtt tttgatgata gaggatgtac aatttggcat
     5461 acttgtggaa aataaatcta aagtattttt aaatgcaatc tttttagtcc aatttaaggt
     5521 gtcattttct attttacatg gaaagtcgat attatataaa tcccaactgt taaacttaga
     5581 actattataa atattaaaaa ttttaagcat cttatctgtt tgttctttgt caaaatttat
     5641 ttttagattg tttaatctaa tatagggaat tgtaggatta ttaaaaaaat aagtatctat
     5701 atttttaatt ttttctatgt atttatcaat tgaatcatca acacctatat atcttgtaaa
     5761 gtagacttct ttaatatatg aagaactctt atttttatag gtagtatcag atataaattc
     5821 aattagaggt tcagtagact tttttattga aatctttaat ggtttaatag tacaaatcat
     5881 attttttctc ctataataag atgatataat ctattataaa acaacttaaa acaatatagt
     5941 acaataattt tgtagtttca aaataaaata tgtacttgtt tatataaatg atatataatt
     6001 acaataaaat tgccgaaaag ccctctcctt taggtgtagg gatgaaaggt aaggtcttaa
     6061 tataaaaaaa gttagaataa tgcttgtaat ctaataccgc tactgatacc ctatatttag
     6121 g
//
LOCUS       DAELLV010000211         6114 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.211, whole genome shotgun sequence.
ACCESSION   DAELLV010000211 DAELLV010000000
VERSION     DAELLV010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6114
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.211"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(119..2317)
                     /gene="slpA"
                     /locus_tag="KPD58_003062"
     CDS             complement(119..2317)
                     /gene="slpA"
                     /locus_tag="KPD58_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein SlpA"
                     /protein_id="HBG0266793.1"
                     /translation="MNKKNLAMAMAAVTVVGSAAPIFADTTVKEEGYTVVQDKYEKLL
                     KELKAKIKDGTITSVGVEFDGKPITTLAPKADGSDKDAIAEQLETLTKNQLKGLGDGK
                     YVDFKITYGAKAEVPAASLSADDIQKYADQINASEKILVEVAAGSEAGIAKFDSVNNK
                     VIAGDAPLKVKDAVKATVTTNGSNKKVLTISAAAGLSGFSYGTLKDTGASLSDVDAIT
                     LDTTNATITEGDTKVLDFDNSFKFNESTKKVGSLVTPNTTNTPADPGTKTTVRVIKAV
                     EKTIDVSSNSTTKAKDLAKQYVFTDVSDTDPESLSYMLKNINDGKVAVKNSDGDYEVT
                     IFPEGKRLNTLSASSAKTILGDKDTPAKIVLKASTTKKLADYIDDLITYNNSYSNVQT
                     VAGSDRIETAIELSRKYYNSTDKNALYGDPVNNVVLVGSQAIVDGLVASPLAAEKDAP
                     LLLSSKDKLDSSTRAEIKRVMDLNSSTGIKNNKEVFIAGGVNSISKDVENELKDMGLK
                     VTRLSGDDRYETSLAIADEIDINDKAYVVGGTGLADAMSIAPVASQIKDGEATPIVVV
                     DGKSDKLSKEAEDFLDDAQVDIIGGENSVSAKMEDYIDDATGKSPERISGADRQATNA
                     EVIKEYFDKDGVSNYFLAKDGSTKEDQLVDALAAAAVAGNYGSKHNEDGDITTDASPA
                     PIILATDNLSAEQHVAVSKTATTNGAKNLVQVGQGIADSVVSKLKDLLDM"
     gene            complement(2607..4205)
                     /locus_tag="KPD58_003063"
     CDS             complement(2607..4205)
                     /locus_tag="KPD58_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG0266794.1"
                     /translation="MKVNKRVLSIGLTISLIMAGAPNINALSSIEKIQGKDRYETSAL
                     IADKQIYDTIILVNTDNSIVDGLSASGLSGVAKAPIMLVQRDKIPTDVEKRLKDVKNA
                     YVIGTEDTIGKSVENQLKNKGIEVKRIGGEDRIKTSYLIAKEISAIKPVNDGDKVFLV
                     NGYTGEADAMSVSSVAARDGVPVILTDGKSIPFKVDGVQCYSLGSEEIMSNELVSKTN
                     SVRIAGKDRFETNKKVIQRFYKGTKKFYVSQGYKLVDAVAGSPLAKDKPIVLVNDGSD
                     KSVLRGADEVTSLGGMDKKVVDQCISSASDKNTMPTITANDVEISVGDKFDNSMLNIV
                     ATDYYGNDLKANIKGNVDINKAGTYVLNISVVDNLGQKSEISVNVKVVVNASTKDSNS
                     YEFKAMVSNEMYDLVNSYRKEKGKKSLRELDSLAGMANAWSKYMEDKKVFAHEIDGKN
                     AAEVFFGFGARSGENIAYLPMNVKSVYTSKDAKEMAESIFDLWKKSSKYNENMLKEEF
                     YSFGFGMHVSSKGEVNATMEFLNS"
     gene            complement(4424..6025)
                     /locus_tag="KPD58_003064"
     CDS             complement(4424..6025)
                     /locus_tag="KPD58_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp11"
                     /protein_id="HBG0266795.1"
                     /translation="MKMNKKILSLGLAVSLILVNFKSVNASSVVEKIYGKDRYETAAK
                     IADKQTYETVILVNTEKSLADGLSASGLSGATKAPILFTQQNKIPADTNRCLKNIKKA
                     YIIGTEDTISKSVEKELDSKNIEVKRIGGEDRLKTSYLIAKEIATIKKVDKVLLTNAY
                     SGEADAMSVSSVATRDGAPIILTDGKSVPFDVKNIQSYCIGSEEIMSNPLVKNTNSVR
                     IEGTDRFETNKNVIDYFFNSADGFYVSDGYQLVDAIAAAPLTKNSPMVLVNDGSDKTV
                     LEGAKNITSVGEINEKVIQQCINASNSNGQPPTITVGSTEVYKGEKFDTGKLNIVAKD
                     NTGKVLPIEVDGFIDTNRVGTYILTLKATDEWGKSTGKRVEIKVLDDKSHDYNSPEFK
                     KMVSTEMYNLINSYRKEKGKEPLLVSSRLEGMANAWSKYMMDKKVFAHYIDGKNAPQV
                     FSEFGMRSEENIAYIYIDSKNVQTTQDAKDLAKAIFEVWKKSPEYNANMLSNEFYSTG
                     FGLYILSDGQVHATQEFLNGNEGSL"
BASE COUNT         1809 a         1209 c          676 g         2420 t
ORIGIN      
        1 gtgtttctca cattaaaaag aggctaatta gcctcttttt atttataaaa atttatgttt
       61 aaaataaaaa aggtttctca aaagagaaac cttttttatt caatatataa aatttatatt
      121 acatatctaa taaatctttt aatttgctaa caactgaatc agctattcct tgacctactt
      181 gtactaagtt cttagcacca ttagtagtag cagttttact tacagctaca tgttgttcag
      241 ctgataaatt atcagttgct aaaataattg gagcaggaga agcatctgta gttatatctc
      301 catcttcatt atgcttagaa ccgtagtttc cagctactgc tgctgctgct aatgcatcaa
      361 ctaactgatc ctctttagtt gaaccatctt ttgctaagaa gtaatttgaa actccatctt
      421 tatcaaagta ttcttttata acttcagcat tagttgcttg tctatcagca ccactaattc
      481 tttctggaga ttttccagta gcatcatcta tataatcttc cattttagca gaaacactat
      541 tttctccacc gattatatct acttgtgcat catctaagaa atcttcagct tctttagata
      601 acttgtcaga ttttccatct acaactacta ttggagtagc ttcaccatct tttatttgag
      661 atgcaactgg agcaatactc atagcatctg ctaatcctgt tccacctacc acataagctt
      721 tatcatttat atctatttca tcagctatcg ctaatgaagt ttcatatcta tcatcacctg
      781 ataatctagt tactttaagt cccatatctt ttagttcatt ttcaacgtct ttagatatag
      841 aatttactcc acctgctatg aaaacttctt tattattttt tattcctgtt gaagaattta
      901 agtccataac tctttttatc tcagctcttg ttgatgaatc taatttatct ttagaactta
      961 acaataaagg agcatctttt tctgctgcta aaggagatgc tactaatcca tcaactatag
     1021 cttgagaacc tactaataca acgttattta caggatctcc atataaagca tttttgtcag
     1081 tagagttata gtacttacgg cttagctcta tagcagtctc tattctatca ctacctgcta
     1141 cagtttgtac atttgaataa ctattgttat aagttattaa atcatctata tagtctgcta
     1201 atttctttgt tgttgaagcc tttaaaacta tttttgctgg agtatcttta tctcctaaaa
     1261 tagttttagc tgaagaagca gataaagtat ttaatctttt tccttctggg aatatagtta
     1321 cttcataatc tccatcagaa tttttaacag caacttttcc atcatttatg tttttaagca
     1381 tataacttaa actttctgga tcagtatcac taacatctgt gaatacatat tgtttagcta
     1441 aatctttagc ttttgtagtt gaatttgaac ttacatctat tgttttttca acagctttta
     1501 tcactctaac agtagtttta gtaccaggat ctgctggagt gtttgttgta tttggtgtaa
     1561 ctaaactacc aactttttta gtactttcgt taaacttaaa tgaattatca aaatctaata
     1621 ctttagtatc accttctgtt attgttgcat ttgtagtgtc aagagttatt gcatcaacat
     1681 cagataaact tgcacctgtg tctttaagtg ttccatatga aaatcctgat aatccagctg
     1741 cagctgatat tgtaagaact tttttattac taccattagt agtaacagtc gctttcactg
     1801 catctttaac tttaagagga gcatctcctg ctataacttt attgttaaca gaatcaaatt
     1861 ttgctatacc tgcttcagat cctgctgcta cttcaactaa tattttctca gaagcattta
     1921 tttgatctgc atatttttgt atatcatctg cacttaatga agcagctgga acttctgctt
     1981 tagcaccata agtaatttta aaatctacat attttccatc acctaaacct ttaagttggt
     2041 ttttagtaag agtttctaac tgttctgcta ttgcatcttt atcactacca tctgcctttg
     2101 gtgctaatgt agtaattggt tttccatcaa attcaacacc aactgatgta atagttccat
     2161 cttttatctt tgcctttaat tctttaagta atttttcata tttgtcttgt actaccgtat
     2221 atccttcttc tttaacagta gtatcagcaa atatcggagc agcagaacct acaacagtaa
     2281 cagctgccat tgccattgct aaatttttct tattcatttc ttaaattcct cccaacatta
     2341 tattaattac atctctttaa ttatgtacat taaatttcca tgttcatata atccaaatat
     2401 ttctcacact cttatgatag actatataaa atataaattc aataatttat gcaaaattaa
     2461 ttgaagtttt ttccaacaaa ttaattgttt tttcatcatt tttattattt ttaaattatt
     2521 ttttataatt ttataagtta ttttaatttt atatatttat ttttacaaat ttactatatc
     2581 ttttccaatg taaattaata taaaatctaa ctatttaaaa attccattgt cgcattcact
     2641 tctcctttac tagaaacatg cataccaaat ccaaagctat aaaattcttc ttttaacata
     2701 ttttcattat acttagaaga cttcttccat aaatcaaaaa tagactcagc catttctttt
     2761 gcatctttag aagtataaac agatttaaca ttcattggta gatatgcaat attttcacca
     2821 cttcttgctc caaagccaaa aaatacttca gctgcatttt taccatcaat ctcatgggca
     2881 aataccttct tatcttccat atatttagac catgcatttg ccattccagc taaagagtct
     2941 aactctctta aactcttctt gcctttttct tttctatatg aattaactaa atcatacatc
     3001 tcattagata ccatagcctt aaactcataa ctattagagt cttttgtact cgcatttaca
     3061 actaccttaa catttacact aatctcactc ttctgaccta aattatccac tacacttata
     3121 tttaaaacat atgttccagc cttgttaata tcaacatttc ctttaatatt tgcctttaaa
     3181 tcatttccat aataatcagt agcaactata ttcaacatac tattatcaaa cttatcccct
     3241 acagaaatct ctacatcatt agcagtaatt gtaggcattg tatttttatc agatgcagaa
     3301 ctaatacatt ggtcaacaac cttcttatcc attccaccta aagaagtaac ttcatcagca
     3361 cctctaagca cactcttatc acttccatca ttaacaagta ctataggttt gtccttagcc
     3421 aacggagaac cagccacagc atcaactaac ttataccctt gagaaacata aaacttcttt
     3481 gttcctttat aaaagcgttg tataaccttc ttattagttt caaacctatc cttaccagca
     3541 atcctaactg aattagtctt actcacaagt tcattactca ttatctcctc tgacccaaga
     3601 gaatagcact gaacgccatc aactttaaat ggtatactct ttccatcagt aagtatcaca
     3661 ggaactccat ctctagcagc tacagaagat acactcattg catcagcctc acctgtgtat
     3721 ccatttacaa gaaaaacctt atcaccatca tttactggtt taatagctga aatctcctta
     3781 gctataagat agctagtctt tattctatct tccccaccaa ttcttttaac ttcaatccct
     3841 ttattcttaa gttgattttc tacagattta cctattgtat cctctgtacc tataacataa
     3901 gcattcttaa catcctttag tcttttttca acatctgttg gtatcttatc tctttgcacc
     3961 aacattatag gagcttttgc aactccagat aatccacttg cacttaatcc atctactata
     4021 gaattatcag tatttactaa tatgattgta tcatatattt gtttatctgc tatcaatgca
     4081 gaagtctcat atctatcttt cccctgtatc ttttctatgc tcgaaagtgc attgatattt
     4141 ggtgctcctg ccattattaa tgatattgtc aatcctattg ataatactct cttatttacc
     4201 ttcatcaaac tcctcctact aattttttta tataattcag ctaatcataa attacatcct
     4261 cagtagtgat tccctcaaaa tctataaact actaaaacaa aaaacaccta aaataccata
     4321 gcctgtaaag caataacaaa acagattaca aaaagtactt tagatgtgat tttcatttta
     4381 taatatcagt aaattaacaa aaaaacaaga tttatggaat tttttacaag ctaccttcat
     4441 ttccatttaa aaactcctga gtagcatgca cttgaccatc agataatata taaagcccaa
     4501 atccagtact ataaaactca ttactaagca tgtttgcatt gtactctgga gacttcttcc
     4561 acacctcaaa tatagccttt gccaagtctt ttgcatcttg tgtcgtttga acgttcttag
     4621 aatctatata aatatatgct atattttcct cacttctcat tccaaattca gaaaatactt
     4681 gtggagcatt ttttccatct atataatgtg caaatacttt tttatccatc atatacttag
     4741 accatgcatt tgccatacct tctagcctac tagacactag caaaggctct ttgccttttt
     4801 cttttctata ggaattgatt aagttataca tttcagtaga taccattttt ttaaattctg
     4861 ggctgttgta atcatgagat ttgtcatcca acacttttat ttctactctt tttcctgtgc
     4921 tttttcccca ttcgtctgta gcttttaatg tcaatatgta tgtacctact ctatttgtat
     4981 ctataaaccc atcaacttct attggtaata ccttccctgt gttatcttta gctactatat
     5041 ttaacttgcc agtgtcaaac ttttcaccct tatatacctc tgtacttcca actgtaattg
     5101 ttgggggttg cccatttgaa tttgaagcat ttatacactg ttgtattacc ttctcattta
     5161 tttcacctac agaggttata ttcttagctc cttctaatac agttttatcg cttccatcat
     5221 ttactagtac cattggagag tttttagtaa gtggtgcagc tgctattgca tctactaatt
     5281 ggtatccatc tgatacatag aatccatctg cactattaaa aaagtagtct attacgtttt
     5341 tgttagtttc aaatcggtca gttccttcta ttcttactga gtttgtattt ttaactagag
     5401 gattactcat tatctcctct gaacctatac aatatgattg aatatttttt acatcaaaag
     5461 gcacactctt tccatctgta agtatgattg gagctccatc tctagtagct actgatgata
     5521 cactcattgc atctgcttct ccactatatg cattagttaa tagtacttta tccacttttt
     5581 ttatagttgc tatttcttta gctattaaat agcttgtttt tagtctatct tctccaccaa
     5641 ttcttttgac ttctatattt ttagaatcta gttctttttc tactgattta cttatagtat
     5701 cttctgtacc tattatatat gcttttttga tattttttag acatctattt gtgtctgctg
     5761 gtatcttatt ttgttgtgta aatagtattg gagcttttgt agctcccgat aatccacttg
     5821 cacttaatcc atctgcaagt gatttttctg tatttactaa aattactgtt tcataagtct
     5881 gtttatcagc tatctttgct gctgtttcat atctatcttt accgtatatt ttttctacta
     5941 ctgatgatgc atttacactt ttgaagttta ctaagattaa tgatactgct agacctaatg
     6001 ataatatttt tttgttcatt ttcatatccc cctgatgatt tttatatttt atatttatat
     6061 tgtgattatt ttccatttat tttctcttta ttttccatat attatagagt atat
//
LOCUS       DAELLV010000212         5915 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.212, whole genome shotgun sequence.
ACCESSION   DAELLV010000212 DAELLV010000000
VERSION     DAELLV010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5915
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.212"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            171..1283
                     /locus_tag="KPD58_003065"
     CDS             171..1283
                     /locus_tag="KPD58_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG0266796.1"
                     /translation="MNAFIRKRNKNYVVYLEFEDEKTGKRKQKNMGTFDKKREASKRL
                     NEVKESLYNDGFLVPSEITVSEFLLDFLNKYSENISLATYNNYVSICKNYINPSIGKY
                     KIQDLHPVHIQNYIDKLSYKLNPQSIKIHINILKLAIKRAYRLKLIKENVMDSIEAPR
                     YKKFKNEIYDREQMIKLLDLAKNTEMELPINLAIGLGLRISEVLGLTWDNIDFEENTI
                     TVNKITSRINGSVILKEPKTESSVRKISSPKELMSLLKEYKIKQNKNLLKSSIRNNNN
                     LLFFNKKCEPIAEDVMSKKFKRFLEKNELPHIRFHDLRHSHVTLLINSKVPIKVISER
                     VGHSNISTTLSVYSHVLKEMDKEASDKISESLFNAN"
     gene            complement(1491..1967)
                     /locus_tag="KPD58_003066"
     CDS             complement(1491..1967)
                     /locus_tag="KPD58_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266797.1"
                     /translation="MVKKRLVAVVISLAMISVGNFSYAHSGGHNSSGGHHNKNKSELT
                     RYYCGGHSAHQHNKGFCPYASTKENSGVTISSVHNEKNHKNYFAEKGYNKGYEDGCKG
                     LYNLSVTEAKSDNLYEKEILKSSYEKGYLIGYNEYKAKKVDDVMKKMLDIPMLASI"
     gene            complement(2166..3791)
                     /locus_tag="KPD58_003067"
     CDS             complement(2166..3791)
                     /locus_tag="KPD58_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0266798.1"
                     /translation="MLQVTGVGLRFGDKELFKDVNLKFTKGNCYGIIGANGAGKSTFL
                     KILSGEIEPNTGSISITDKERMSVLKQDHFEYEEETVLNVVIRGHERLWNIMKEKDAL
                     YMKEDFNEEDGIKAAELEGEFAELDGWDAETNAEKILMGLGITKDMHYKQMKELVGGE
                     KVKVLLAQSLFGKPEILLMDEPTNHLDFKSINWLNNFIMDLEESIVIIVSHDRHFLNQ
                     ICTNIVDVDFGKIQMYVGNYDFWYESSQLALQLAKDQNKKTEEKIAQLKEFIARFSSN
                     ASKAKQATSRKKQLEKLEVEDIQPSRRRYPYVGFTPAREIGNEVLEVHNLTKTIDGVK
                     VLDNVSFRLDRDDKVVFMGDEIATTALFNIVMGELEPDSGEYKWGVTTSQDYLPKNHN
                     KFFDGVEYSLVDWLRQFSEEKSESFIRGFLGRMLFSGEEALKEAQVISGGEKVRCMLS
                     KLMLSNANVLVLDDPTNHLDLESITSVNKGLEKFPGVLLFTSHDHEFISTIANRIIEI
                     TPNGIMDRKMDFDEYLESKEIQDQLAKMYGKDK"
     gene            complement(4129..5148)
                     /locus_tag="KPD58_003068"
     CDS             complement(4129..5148)
                     /locus_tag="KPD58_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HBG0266799.1"
                     /translation="MKLIKTIDAVGQVLCHDITQIIPGEFKGRKFKKGHIVKEEDIPV
                     LLSLGKDNLYVWEKSEGMVHENEGALFLKELTAGENLDFSEIKEGKIDFIAACDGLLK
                     IDVDALFDLNCVGEIMMATLHNNFIVNKGLKVAGTRVIPLVIDEKKLEDAKKVVGNRK
                     IVNVVPFKPKKVGIVTTGNEVFYSRIVDKFGPVIEEKVKGFGCEVIGQTICPDDKEII
                     KSAIKEFISQGAELICCTGGMSVDPDDVTPTAIKETGADLVTYGSPILPGAMFLLAYY
                     GEVPIMGIPGCAMYHKTTVFDIVLSRVLIDEKLDKYDIARYGHGGLCMNCDVCTYPAC
                     NFAKI"
     gene            complement(5145..5714)
                     /gene="mocA"
                     /locus_tag="KPD58_003069"
     CDS             complement(5145..5714)
                     /gene="mocA"
                     /locus_tag="KPD58_003069"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HBG0266800.1"
                     /translation="MINAVIMASGFARRMGANKLLLNYKGCSIIEHVFREVSKIDFHQ
                     VVVVSQYEEVLLLCNKYGFEYIDNKNANIGQSESIKLGVLNSSKCDGYMFFVGDQPFI
                     DNLYIDKIINTFKLDRDFIVIPRYKDMCGNPVIFPYSKKEELLALKEDEKGKTILKNS
                     SKIKYVEVPKEMLFDIDTKMDYERIGGKL"
BASE COUNT         2023 a          992 c          600 g         2300 t
ORIGIN      
        1 ctataagtat aacagattat caaattaaaa ttctaatatt tgacataata tatacatttt
       61 ttaaataaat atatacaatg tattaaaatg tatgcaactc tatttcaaaa ttattttgtg
      121 atatatatat gttataataa atgtataaaa ttttgattgg tgggttatat atgaatgcat
      181 ttattagaaa aagaaataaa aactatgttg tttacctgga atttgaagat gaaaagacag
      241 gtaaaagaaa acaaaaaaat atgggtactt ttgataaaaa aagggaagca agtaaaagac
      301 taaatgaggt aaaagaaagc ttatataatg atggtttttt agtaccaagc gaaattacag
      361 tgtcagaatt tctattagat tttctaaata aatatagtga aaatatatct ttagctacat
      421 ataataatta tgttagtatt tgtaagaatt acattaatcc atctattgga aaatataaaa
      481 tacaagactt acatccggtt cacatacaaa actacataga taaactatct tataaactaa
      541 atcctcaatc aattaagatt catataaata tattgaaact tgcaataaaa agagcttata
      601 gactcaaatt aataaaagaa aatgttatgg atagtataga ggctccaaga tataaaaaat
      661 ttaaaaatga gatttatgat agagaacaaa tgataaaatt attagattta gcaaaaaata
      721 cagaaatgga attacctatt aatttggcta ttggattagg tcttagaatt tcagaagttt
      781 taggtctaac ttgggataat attgattttg aagaaaatac aataacagta aataagataa
      841 cttcccgaat taatggttct gttattctca aagagccaaa gacagaaagc tctgttagaa
      901 aaatatcttc tcccaaagaa ttaatgtctt tattaaaaga atataaaata aaacagaata
      961 aaaatttatt aaaatcttcc attagaaata ataataattt gttgtttttc aataagaaat
     1021 gtgaacctat tgctgaagat gttatgagta aaaaatttaa aagattttta gaaaagaatg
     1081 aattacctca tattagattc catgatttaa gacactcaca tgttacttta cttataaatt
     1141 ctaaagtacc tataaaggtt atatccgaga gagtaggaca ctcaaatata agtactactc
     1201 taagcgttta ttctcatgta cttaaagaaa tggataaaga ggcttctgac aaaatttctg
     1261 aaagtttatt taatgctaat taatattaat ttatcttagc tttaaattag cttagcttat
     1321 tgagatagat tcattaatat taaatgtatc tatctcaaat taataactac ttaaataatc
     1381 tgttttattt taatttatcg cctcagttat tatacttttt tacgaaaaat tatactttac
     1441 aataaaaaag atattccctt atagaaaata tctttttaaa tactatatta ttatatactt
     1501 gctaacattg gaatatctaa catcttcttc attacatcat ctactttttt tgctttatat
     1561 tcattgtatc caattaaata tcctttttca tatgaacttt ttaaaatctc tttttcatat
     1621 aaattatcag atttggcttc tgtgacactt aaattataaa gccctttaca cccatcttca
     1681 taacctttat tatacccttt ttctgcaaaa tagtttttat gatttttttc gttatgtact
     1741 gaagatattg ttactccaga attttctttt gtactagcat aaggacaaaa acctttattg
     1801 tgttgatgtg ctgaatgtcc tccacaataa taacgtgtta actctgattt atttttatta
     1861 tgatgacctc ctgatgaatt atgccctcct gagtgagcat atgaaaaatt ccctacacta
     1921 atcatagcta aactaattac tacagctact aaccttttct taaccataat ctattccccc
     1981 caaaatgtta tatattttta ttatatcatc tactatatta tatttaaatt cttaatatac
     2041 aaaatattac tctattctgc taataaacat gtttaaacaa cataaaaaac ctcctaatat
     2101 ttgattaaaa atttacacac tcatgcatag catgtctcaa atattaaaag gttttatact
     2161 aataattatt tatcttttcc atacatttta gctaattggt cttgtatctc tttactttct
     2221 aaatactcat caaagtccat ttttctatcc attattccat ttggagttat ctctataatt
     2281 ctatttgcta tagtagatat aaattcatgg tcatgagaag taaataaaag cactcctggg
     2341 aacttttcaa gtcctttatt tacagaagtt atactttcta ggtctaagtg gttagtaggg
     2401 tcatctaata caagtacatt tgcatttgaa agcattagct tactaagcat acatctaacc
     2461 ttttctcctc cagatataac ttgagcttct tttaatgctt cttcacctga gaataacatt
     2521 ctacctaaaa atcctcttat aaaactttca cttttttctt cagagaactg tctaagccag
     2581 tcaaccaaag aatattctac tccgtcaaaa aacttattat gattttttgg aagataatct
     2641 tgagaagtag ttactcccca tttatactct ccactgtctg gttcaagttc tcccataact
     2701 atgttgaaaa gtgcagtagt agctatttca tctcccataa atactacttt atcatctcta
     2761 tccaatctaa atgacacatt atcaagaacc ttaaccccat ctatagtttt agttaaattg
     2821 tgtacttcta aaacttcatt cccaatttct cttgcaggtg taaatcctac atatggatat
     2881 cttcttcttg atggttgtat atcttcaact tctaatttct ctaactgttt ctttcttgat
     2941 gttgcttgtt tagcctttga agcattagaa ctaaatctag ctataaattc ctttaattga
     3001 gctatctttt cttcagtctt tttattttgg tccttagcca attgtaaagc caattgactt
     3061 gactcatacc agaaatcata gtttccaacg tacatttgta ttttaccaaa atctacgtca
     3121 actatattag tacatatttg atttaggaaa tgtctatcat gtgatactat tataactata
     3181 gattcttcta aatccattat aaaattattt aaccagttta ttgatttaaa atctaagtgg
     3241 tttgtaggtt cgtccattaa tagtatttca ggcttaccaa aaagtgattg tgctaacaat
     3301 accttaacct tttccccacc aactagttcc ttcatttgtt tgtaatgcat atctttcgtt
     3361 atgccaaggc ccataagtat tttctcagca ttagtttctg catcccatcc gtcaagctct
     3421 gcaaactcac cttcaagttc ggcagctttt atcccatctt cttcattaaa gtcttccttc
     3481 atatacaatg catctttttc tttcattatg ttccaaagtc tctcatgacc tcttataaca
     3541 acatttaaaa cagtttcttc ttcatattca aagtggtcct gttttaaaac tgacattctt
     3601 tctttatcag ttatagatat actacctgta ttaggctcta tttctcctga taatattttt
     3661 aaaaaagtag attttcccgc accattagct cctattattc catagcaatt acctttagtg
     3721 aattttaagt taacatcttt aaatagttcc ttatcaccaa atctaagtcc aacacctgta
     3781 acttgtaaca ttaatatatt ttcctttctt tttgctcata atttatttta tcataaaact
     3841 ttgataacat ttttattata tattatcacg taaatagtat attttttata ctaaatttgt
     3901 gaaatattta ttaaaataat actaaaaatt gtaaatttat aaaaattaac aatctcttat
     3961 attatactta taaagaaatc aaattgcaac tttattcatt cttttacaca ttgaaaataa
     4021 gttattataa tttctaaata aatatgcttt tcttaaaaca tcattctagc tgttttgttt
     4081 catatttaaa aagagtactt aatttgtaaa tactcttttt taataacttt atattttagc
     4141 aaaattacat gcaggataag tacatacatc acagttcata caaaggccac catgaccata
     4201 tctagctata tcatacttat caagcttttc atcaattaaa accctagata aaactatatc
     4261 aaatactgta gttttgtgat acattgcgca tcctggtata cccataattg gaacttctcc
     4321 ataataagca agtaaaaaca tagctcctgg taatattggt gaaccatatg tcactaaatc
     4381 agctccagtt tcttttatag ctgttggtgt aacatcatct gggtctacac tcatcccacc
     4441 agtacaacag ataagttctg ctccttgact tataaattct tttattgcac tctttataat
     4501 ctctttatca tctgggcata tagtctgtcc tataacttca caaccaaagc cttttacttt
     4561 ttcttctatt acgggtccaa atttatcaac aattctgcta taaaacactt catttcctgt
     4621 agtaacaatt cctactttct taggtttaaa aggtactaca tttactattt ttctattacc
     4681 aactactttt tttgcatctt ctaatttttt ctcatctata accaaaggta tcactcttgt
     4741 tccagctact tttaaacctt tatttactat aaaattatta tgaagagtag ccatcattat
     4801 ttctccaaca caattcaaat caaaaagtgc atcaacatct atctttaata gaccatcaca
     4861 tgctgctata aaatctattt tcccttcttt tatttcacta aaatctaaat tttctccagc
     4921 agttagttct tttaaaaaca atgcgccttc attttcatga accattcctt ctgacttttc
     4981 ccatacatac aaattatctt taccaagaga taataacact ggaatatctt cttcttttac
     5041 tatgtggcct tttttaaatt ttcttccttt aaattctcca ggtattattt gagttatgtc
     5101 gtgacataaa acttgtccaa cagcatctat agtttttatt agcttcataa tttaccccca
     5161 attctctcat agtccatttt tgtatcgata tcaaataaca tttctttagg aacttctaca
     5221 tactttattt ttgatgaatt ttttaaaatg gttttacctt tttcatcttc tttaagtgcc
     5281 aacaattctt ctttcttgct atatgggaat atgactggat ttccacacat atctttgtat
     5341 cttggaatta ctataaaatc tctatctaat ttaaaagtat ttataatttt atctatatat
     5401 aagttatcaa taaatggctg gtctcctaca aagaacatat aaccatcaca ttttgagcta
     5461 tttaaaactc ctaattttat agattcactt tgacctatat ttgcattttt attatctata
     5521 tattcaaaac catatttatt acacaataac aaaacctctt catattgact tactactaca
     5581 acttgatgaa aatcaatctt gctaacttct ctaaatacat gttctattat agaacagcct
     5641 ttataattta ataaaagttt atttgctccc attcttctgg caaagcctga tgccattatc
     5701 actgcattta tcatatatat catccttggt atttataata tattgatagt tctatgattt
     5761 ttataatatc tctattgata ttatctatat atataatatc atataaacta ttttaatact
     5821 tcaatatctt acacaaaatt tttgttttac acttccataa aatatctcta tttcactttc
     5881 atattcttta atattttcta ttaattttat agcta
//
LOCUS       DAELLV010000213         5912 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.213, whole genome shotgun sequence.
ACCESSION   DAELLV010000213 DAELLV010000000
VERSION     DAELLV010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5912
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.213"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            44..1708
                     /locus_tag="KPD58_003070"
     CDS             44..1708
                     /locus_tag="KPD58_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HBG0266801.1"
                     /translation="MSNETNSSNFIKNIIINDLETGKHDSIITRFPPEPNGYLHIGHA
                     KSICLNFGLAKEFNGKANLRFDDTNPLKEDVEYVESIKEDVKWLGFDWNELNFASNYF
                     DEMYKRALILIKKGKAYVCDLTQEEMREYRGTLTEPGKESPHRNRTIEENLDLFERMK
                     NGEFKDGEKTLRAKIDMSSPNINLRDPIIYRISHSTHHNTGDKWCIYPMYAFAHPIED
                     AIEGITHSICTLEFEDQRPLYDWFVKECEMENIPRQIEFARLNINNTVMSKRKLKQLV
                     DEGIVDGWDDPRVPTISGIRRKGYTAEALRNFCSEIGVSKVNSTVDSQMLDYFLRENL
                     QPKAPLTMGVLRPLKLIITNYPEDKIEMLEIENNAKDESQGKRLVPFSRELYIEQDDF
                     MEEPVKKYFRFFPGNEVRLKGAYFVKCTDVIKDENGNVVEIHGTYDPETKSGSGFTGR
                     KVKSTIHWVDAKSAIPCEFRLFEPLILDDIPENEGKHFLEQINPNSLEILQGFVEPTQ
                     IKDAKPFDKFQFVRNGFFSIDNKYTTDEKFVFNRIVPLKSSFKPGK"
     gene            complement(1815..2714)
                     /locus_tag="KPD58_003071"
     CDS             complement(1815..2714)
                     /locus_tag="KPD58_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HBG0266802.1"
                     /translation="MKKVKLIYNPNSGEKKILSNLDTIIELYQEKNYLLIPYRLTIKE
                     PVKNAFKEVDSSYEHILIAGGDGTVDLVVNAMKELDIKIPIGILPTGTANDFSNALQI
                     SFDIKEAINEIINSKPKKIDIGKVNNKYFINVASAGMFTDVSQRINTDLKNSFGRISY
                     YIKGIEEALYMRKFKIKVSSEEMYYDGDMYLMLIFNGKTAGNINLAYKAEIDDGYLDV
                     IIFKGMPIPKSIPVLISVLRGDYLDQYNGNEILYFKTKKVHIECEDSLITDIDGEKGP
                     DFPLNIECIKDGIEVMGICSEKS"
     gene            complement(2741..3379)
                     /locus_tag="KPD58_003072"
     CDS             complement(2741..3379)
                     /locus_tag="KPD58_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HBG0266803.1"
                     /translation="MDQNLNWLNEKRIEKTIKSLENNNMKGYLVSNKEELLKKIEEIV
                     KAGSLVGCGGSMTLFETGVIDFIREKYNFLDRYAKDIKPEEMKEIYRKSFSADAYFTS
                     TNAITEDGELYNLDGNGNRVAAMIYGPDKVIVIAGVNKIVKDVDEAIKRTKTIAAPAN
                     CKRLSTNTGCKTTGICIDCSSPGRICCSYTLIKRQLTPERIHVIFLNEDFGY"
     gene            complement(3720..4718)
                     /locus_tag="KPD58_003073"
     CDS             complement(3720..4718)
                     /locus_tag="KPD58_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4003 domain-containing protein"
                     /protein_id="HBG0266804.1"
                     /translation="MEQSFINGKVNLLIENYKALNEVKGSWQMGLIQHSCALAFTLKN
                     KRISPRLVEERIELIKKNTGLFSNFRGYNMFYMATLLSFESNPESSFKMILDIYKELK
                     SEKFWGDTYLPLTASIVYENREKMDYLTCISKMKIIYDYMRKKHPFLTSSDDYCNIAL
                     IAIHSKNLDEDLEYIEKCYEFLNENGFYKGNDLQALSQILLFSDDRTMLKCKKTIELK
                     KAFKENDCKMNYYGYPIIGAISLLDYREDEIIEEIKNVSNKLKEEKGFGNWSLGKSNR
                     VMISSAIVASIYADLIRNENNIGSITNNIFLNIVVAIQIACVMAATSAAIASSSSN"
     gene            complement(4816..5391)
                     /locus_tag="KPD58_003074"
     CDS             complement(4816..5391)
                     /locus_tag="KPD58_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266805.1"
                     /translation="MKLKEALITCVVCSVCLTPLLVNGETKIDADHCKESIVNIDTIV
                     KSGKSFNSKYNQGMEDYKNSDEYKLKVQKEKLQNQLGIKIKKLVPITFEVSYYTSLNQ
                     ENGYGAITATGDDLEDGFVANNHLSFGTKIIVDGKLKTVMDRGSSKYFSNSNAIDVFV
                     PKWSNESESEYYDRVNNMGRHYKKGYIIVEE"
     gene            5707..>5912
                     /locus_tag="KPD58_003075"
     CDS             5707..>5912
                     /locus_tag="KPD58_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266806.1"
                     /translation="MIKNTKQITVEVNEILLQEMSKITNDENNILTDLINESIVNYIL
                     ENKKHNIENKDLEEVIKFYKKENT"
BASE COUNT         2047 a          898 c          688 g         2279 t
ORIGIN      
        1 tagtgttctg tattataatc aaattaaata ggaggtatag aatatgtcaa atgaaacgaa
       61 ctcatctaac tttataaaga atattattat aaacgactta gaaacaggaa aacatgacag
      121 tatcataact cgttttccac ctgagccaaa cggatattta catattggtc atgcaaaaag
      181 tatatgtctt aattttggat tagctaagga gtttaatgga aaagctaatt taagatttga
      241 tgatacaaac ccattaaaag aggatgttga atatgtagag tccataaaag aagatgtaaa
      301 gtggttagga tttgattgga acgagttaaa ttttgcatct aactactttg atgaaatgta
      361 taagagagct ttaatactaa taaaaaaggg taaagcttat gtatgtgatt tgacacaaga
      421 ggaaatgaga gaatatcgtg gtactctaac tgaaccagga aaagaaagtc ctcatagaaa
      481 cagaactatt gaagaaaacc ttgatttatt tgaaagaatg aaaaatggtg aatttaaaga
      541 tggtgaaaaa acattaagag ccaaaataga tatgtcttct ccaaatataa accttagaga
      601 cccaattata tatagaatat ctcattcaac acaccataat acaggagata aatggtgcat
      661 atatcctatg tatgcttttg ctcatccaat tgaagatgca attgaaggaa taactcactc
      721 tatatgtact ttagagtttg aagaccaaag acctctttat gattggtttg ttaaagaatg
      781 tgagatggaa aatataccta gacaaataga atttgctaga cttaatataa ataatactgt
      841 catgagtaaa aggaaattaa aacaacttgt agatgagggt attgttgatg gttgggatga
      901 ccctcgtgtt cctacaatat ccggaatcag acgtaaggga tatactgcag aagcattgcg
      961 caatttctgt agtgaaatag gggtatcaaa agttaactct acggtagata gtcaaatgct
     1021 tgattatttc ctaagagaaa acttacaacc aaaggctcct cttactatgg gagttttaag
     1081 acctttaaaa cttattataa ctaattaccc agaagataag attgagatgc ttgaaataga
     1141 gaataatgct aaagatgaat ctcaaggaaa gagattagtt cctttctcta gagaattgta
     1201 tatagaacaa gatgatttta tggaagaacc tgttaagaaa tattttagat ttttcccagg
     1261 aaatgaagtc cgtttaaaag gtgcttattt tgtaaaatgt actgatgtaa ttaaggatga
     1321 aaatggaaat gtagttgaaa tacatggtac ttatgaccct gaaactaaga gtggctctgg
     1381 ctttacagga cgtaaggtaa aatctacaat acattgggtt gatgctaaat cagctatccc
     1441 atgtgaattt agattatttg aaccattaat tcttgatgat atacctgaaa atgaaggtaa
     1501 acatttctta gaacaaataa atcctaattc attggaaata ttacaaggtt ttgttgaacc
     1561 tacacagata aaagatgcta aaccttttga taaattccaa tttgttagaa atggattctt
     1621 tagtatagat aataaatata caactgatga aaaatttgta tttaatagaa tagttcctct
     1681 aaaaagttct tttaaaccag gtaaataatc ttacaatcaa aaaatgaaga cttaaaatat
     1741 aaattacttc tactataaat aaacttagtt agaagtaatt ttttatttga gtcttcatca
     1801 tttactttaa atgattaact tttttcacta cagattccca tgacttctat tccatctttt
     1861 atgcattcta tgttaagagg aaaatctggt cctttttctc cgtcaatatc tgttataagt
     1921 gaatcttcac attctatatg caccttttta gttttaaagt atagtatttc atttccatta
     1981 tattgatcta aataatcacc tctaagcaca cttatcaaaa ctggtattga ttttggtatt
     2041 ggcattcctt taaaaataat tacatccaaa tatccatcat ctatttcagc cttatatgct
     2101 agatttatat ttcctgctgt tttgccatta aaaatcaaca taagatacat atctccatca
     2161 taatacattt cctcagaaga tacttttatc ttaaatttcc tcatatacaa tgcttcttct
     2221 attcccttta tataataaga aattcttcca aatgaatttt ttaagtctgt gtttatcctt
     2281 tgtgatacat ccgtaaacat accagcactt gcaacattta taaagtactt attattgact
     2341 ttccctatgt ctattttctt tggttttgaa tttatgattt catttatagc ttcttttata
     2401 tcaaatgaaa tttgaagagc atttgaaaaa tcatttgcag ttcctgtagg caaaattcca
     2461 attggaattt ttatgtctaa ttctttcatt gcatttacta ctaaatctac agtaccatca
     2521 ccaccagcta tcaatatgtg ttcataagaa ctatcaactt ccttaaatgc attctttaca
     2581 ggttctttaa tagttaatct atagggtatc aataaataat ttttttcttg atataattct
     2641 atgatagtgt ctaaatttga taatatcttt ttttcacctg aattaggatt gtatataagt
     2701 tttacttttt tcattatttc tccttactca ttttattctt ttagtatcca aaatcttcat
     2761 ttaaaaaaat cacatgtatt ctttctggtg ttaattgtct ttttataagt gtataactac
     2821 agcatattct tcctggactt gaacagtcta tacatatacc agtagtctta catccagtat
     2881 ttgtacttaa tcttttacaa ttggctggag cagctattgt ttttgttctt tttatagctt
     2941 catctacatc tttaactatt ttatttactc ctgctataac tattacttta tcaggtccat
     3001 atatcatagc agcaacccta ttcccattcc catctaaatt gtaaagttct ccatcctctg
     3061 ttatcgcatt agtacttgta aaataagcat ctgcagaaaa tgactttcta tatatttctt
     3121 tcatttcttc tggtttgata tcttttgcat atctatcaag aaaattatat ttttctctta
     3181 taaaatcaat cactcctgtt tcaaataagg tcatagaacc tccacatcct actagagaac
     3241 ctgctttaac tatttcttca atttttttaa gaagctcttc tttgttactt actaaatacc
     3301 ctttcatatt attgttttcc aaagatttta ttgttttttc tattcttttt tcattaagcc
     3361 aatttaaatt ttgatccatt ttaacttccc ccttattttt aatatgtact taattattta
     3421 taaactttat tttctttata agtttatttt agtacatcta aaaaaaaata caatataaaa
     3481 cttaaatagt tttacataat tttttccaaa ataacttact caatcaaaaa aggaaagtag
     3541 atgtactatt ctactttccc tatattgtat gtagtattat atattgccta aaatctatat
     3601 attaattata tgtttcatat tatatgaata tcacagttaa ttaaaatgta atactatctt
     3661 ctttttaaac atataaattg tattattaaa tttaatatat ccaacttcaa taaatcttat
     3721 taatttgatg aagaagatgc tatagctgca cttgttgctg ccattacaca tgcaatttga
     3781 atagcaacaa caatatttaa aaatatatta ttagttatag agcctatatt attctcattt
     3841 cgtatcaaat ctgcataaat tgaagctaca atagcagaac taatcattac tctattactt
     3901 tttcctaaag accaatttcc aaagcctttt tcttctttaa gtttatttga aacatttttt
     3961 atctcttcta ttatttcatc ttctctataa tctaataatg aaattgctcc tattattgga
     4021 tatccataat aattcatttt acaatcattt tctttaaatg cttttttaag ttcaatagtc
     4081 tttttacatt tgagcatagt tctatcatca ctaaataaaa gaatttggga taatgcttgt
     4141 aaatcatttc ctttataaaa accattttca tttagaaatt cataacattt ttctatgtat
     4201 tctaaatctt catctaaatt ttttgaatga attgcaataa gtgctatatt acaatagtca
     4261 tcactagaag ttagaaatgg atgttttttc ctcatgtaat catatattat tttcattttt
     4321 gatatacatg taagataatc cattttctct ctattttcat aaactattga tgctgtaagt
     4381 ggtaaatatg tatcgcccca aaacttttca cttttaagtt ctttgtatat atctaaaatc
     4441 atcttaaaac tactttctgg atttgattca aacgaaagta gtgttgccat ataaaacata
     4501 ttatatcctc taaaattaga gaataatcca gtattttttt taataagctc tattcgttct
     4561 tccactaatc taggacttat tctcttgttc ttaagggtaa atgcaagtgc acaactatgt
     4621 tgtatcaatc ccatttgcca agaaccttta acttcattta atgctttgta attttctatt
     4681 aataagttta cttttccatt tataaacgat tgttccatat tgccctccta gtaatatatc
     4741 tcattgtact aatagtttta tatttattat atatatatta aatactacaa tcaatacata
     4801 ttacttcaaa gaacatcatt cttcaacaat aatgtagcct tttttgtaat gtcttcccat
     4861 attattgact ctatcatagt actctgattc actttcatta ctccatttgg gcacaaatac
     4921 atctattgca ttagaattac taaaatattt actagacccc ctatccataa cagtttttaa
     4981 ttttccatct acaataatct tagttccaaa acttaagtga ttgtttgcta caaaaccatc
     5041 ttctaaatca tctcctgtag cagttatcgc tccatatcca ttttcttgat taagacttgt
     5101 gtaataacta acttcaaaag ttataggtac taacttcttt attttaatac ctaactgatt
     5161 ttgtagtttc tccttttgta ctttcaactt atactcatca ctatttttat aatcttccat
     5221 accttgatta tacttggaat taaagctttt acctgacttt actatagtat ctatatttac
     5281 tatagattct ttacaatgat ctgcatctat ttttgtttct ccatttacca ataatggagt
     5341 taaacataca ctacatacaa cacatgtaat caatgcttct ttaagcttca taaattcccc
     5401 ccttattatt ttgatatatt agcatattta atattatatg tctacattta gatttaaata
     5461 ccacacccat ttgctttaag aagttgtctt atatttaatt caccttaaac acttataaat
     5521 cctctatctt agaatcatat agaataaagc ttttatcttt aaatattttt tattaatctt
     5581 ttaaaatcta aaactacttt atctaatttt ctctttttta gaatttaaga attaaaattc
     5641 tataatttta aaacatattg aatttatttt tttgagatga tataattttc ttaagaggtg
     5701 gttgatatga taaaaaatac aaaacaaatt acagtagaag ttaatgaaat tttattacaa
     5761 gaaatgtcta agataactaa tgacgaaaat aatattttga cagatttgat aaatgaatca
     5821 attgtaaatt atattcttga aaacaaaaaa cataatattg aaaataaaga tttagaagaa
     5881 gtgattaaat tttacaaaaa agaaaatact ga
//
LOCUS       DAELLV010000214         5877 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.214, whole genome shotgun sequence.
ACCESSION   DAELLV010000214 DAELLV010000000
VERSION     DAELLV010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5877
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.214"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            134..1051
                     /locus_tag="KPD58_003076"
     CDS             134..1051
                     /locus_tag="KPD58_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBG0266807.1"
                     /translation="MMILIQSRGKMKCKELSEELEVSERQIKSYKMYLEQAGIFINST
                     PGIYGGYEIDKCNSISLIKLLDSEVSILDMINSQLEYNNDIYKNEFNNIVEKIKAVLN
                     TGEKSDTYMDYFTVQAQCNCDYESEKNKCNEIIRAYTTKHKFWIEYYSLNSGNSERIV
                     HPYGLFNYKSDTYMVAFCEKRFKFIDFKLCRIKDYKVLEEKYNVDKSFSWDEYSKNSI
                     GIYKGEEISVVIKISHPFSTIIKEKVWVNNQQIIEYDDKSIMFKAKMRGYEEIKSWIL
                     SMGAYVEVVEPDRLRNDILLEIEKMKKIY"
     gene            1129..1866
                     /gene="cas6"
                     /locus_tag="KPD58_003077"
     CDS             1129..1866
                     /gene="cas6"
                     /locus_tag="KPD58_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HBG0266808.1"
                     /translation="MKMKIEFSTGCIPISYNSLFMSIIKEAIRKSNEDYYKNMYYYKE
                     KNNKKTKNFTFSVYIKKYSIEGDNFIIEDKVILNISTPDLELGFHIYNGLMTSKKCLY
                     KDYELTRIRIDLSREKKVTEERVLFNALSPICVKSKEGKFLEITDDRYIEELNYITNE
                     VVKNYRGNGLKRKLEFENIGLKKVVVKESLREFKKITGKEYQYINGYKGKFILKGDID
                     DLNLIYNLGIGFRRAQGFGDVDILEWR"
     gene            1872..3749
                     /gene="cas8a1"
                     /locus_tag="KPD58_003078"
     CDS             1872..3749
                     /gene="cas8a1"
                     /locus_tag="KPD58_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas8b1/Cst1"
                     /protein_id="HBG0266809.1"
                     /translation="MKLRVYRGDWFFNMGIVGFLNIIKKADKQAEIFIMEDYIEFDSL
                     FLENFHEYYFNYFMDEYDVSKRIKKNIDYSINFIKSKPDRIKDGIKKIKDSVKQQNDK
                     IKKFDEEKFNLIKEKLDSMSKIKSYDEFDSLEALVDETIDIFKIKSINDRLTANLYKY
                     VVQDNYFGQVSFFNVAKAKLDLDGLKQVMFNDYLRQIIYFGELEDLLEENDYDKLKNY
                     LNDRLNSIAKDINEKKVSKSSINTIEKIMKEINKNFIKKNKSIEDIKKYMDSLEVCEM
                     CGLYKGILDEYSESNFAPLGVSTNNARNMFWNQAYVPSICDICKLILFCTPAGATYTR
                     KNYLINEENEFYLFVNMDTCINELFERNNSLKAQKSEYSDSKEENPFNQLIKSIVEEN
                     TLKSEWQLRNILFVEFKASIDSKKCKINYFNIPTYLAKFFTDKYANKKIQSIYDYKLK
                     SNVLDLLLKNRDLKHLINNILRNKVKNDMESNNKSNISGIDCFRVVQLRALINSYKKG
                     VYKMDSKNLEKNDEKLRIIYYLGCDIHDYFVNKNSKNKIGGVSYKLLNSVKVGNKSDF
                     MDTIIRVFMSAEKQMPAFILDIEIEKDLDFESIGHAFISGLISGKYEGKDKLPNEKEE
                     K"
     gene            3751..4665
                     /gene="cas7i"
                     /locus_tag="KPD58_003079"
     CDS             3751..4665
                     /gene="cas7i"
                     /locus_tag="KPD58_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HBG0266810.1"
                     /translation="MKRGLTFTVIAQAQSLNYGEGIGNISELKKLSRDDGNIYTFASR
                     QCLRYDIVRLAAELFNWNLQVVDKEKGTIQFKDNISIRESEEMDLFGYMKTNKKDEKD
                     KKGGSLTREATVRLSNAISLEPYRSDMDFLNNKGFADRIGEHPNLANIEQHLSYYTYT
                     VTIDLSKIGKDGEIELDNKEKCRRVVEFLEIIKVLNRNIRGRQENLSPLFVVGGVYEI
                     ANPFFLGRIKLKGDKNGFKINKQAIEEVIQGTFLGKDLKEFTYVGMVDGVFINKEEFK
                     GLFEDNFFSVDKFFGQLVKEVKEYYGVN"
     gene            4667..5368
                     /gene="cas5b"
                     /locus_tag="KPD58_003080"
     CDS             4667..5368
                     /gene="cas5b"
                     /locus_tag="KPD58_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas5"
                     /protein_id="HBG0266811.1"
                     /translation="MKVLKLDLFQETACYKKPFAMKITETYPLPPYSTINGLIHKILN
                     ATEYIPFNISVQGTYESIFNNYQTTYFYKKDSITSMPMNSHMLLNVNLIIHIGGDFDL
                     LEEIYDSLLNYDEHLSLGRKEDLVRINDIRFVEVNKFEVDEDIQLDDYENYKLSECNI
                     RMPIYIPKTSLQDTDIDGISYRLNNYYDNNAVEDKKRVWNKVDSYYVEDGNTISIGDI
                     LLDNEGDLLYFNNIN"
     gene            5379..>5877
                     /locus_tag="KPD58_003081"
     CDS             5379..>5877
                     /locus_tag="KPD58_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HBG0266812.1"
                     /translation="MLYAKSNPVETLREHTDELLKQMNVLRESYGKNINSLDFLEEEI
                     FWQLLDLVIEFHDIGKAFSPFQELIKSRMDTKINEPKIVTHLENNVGHNYLSPAFIDY
                     SYIDRKKNKELRAVLNQVIVYHHERDIFIDKDFKNLIQKILDEDLINKVYELQHEFKV
                     RYPIKT"
BASE COUNT         2537 a          444 c         1019 g         1877 t
ORIGIN      
        1 aattataaaa aaattctact tatataaaat ataaagaggt tatatgtaca tatgtgtaga
       61 aataaataat aaaaatatca attaatgtta ttaggggggg ttactcatta gtaaattagc
      121 taatacactt agaatgatga tattaattca aagcagaggc aaaatgaaat gcaaagaatt
      181 atcagaagag ttggaagtaa gtgaaaggca aataaaaagt tacaagatgt atttagagca
      241 agctggtata tttataaatt caacacctgg gatttatggt ggttatgaaa tagataaatg
      301 taatagcatc tcattaatta aattattaga tagtgaagtt tcaattctag atatgataaa
      361 ttcacagtta gagtataata atgatattta caaaaatgaa tttaataata ttgtagaaaa
      421 aataaaagca gtattaaata caggagaaaa aagcgataca tatatggatt attttacagt
      481 tcaagcacaa tgtaattgtg attatgagtc tgaaaaaaat aagtgtaatg aaataattag
      541 agcatatacc acaaaacaca aattttggat agaatattat tcattaaatt caggaaatag
      601 tgaaagaatt gttcatccat atggtttatt taattataaa tcagatactt atatggtagc
      661 tttttgtgaa aaaagattta agtttataga ctttaaatta tgtagaatta aagattataa
      721 ggttttagaa gaaaaatata atgtagataa aagttttagt tgggatgaat atagtaaaaa
      781 tagtatagga atttataagg gagaagaaat tagtgttgtt ataaaaatta gtcatccatt
      841 ttcaacaatt ataaaggaaa aagtttgggt taataatcaa caaatcatag aatatgatga
      901 taaatctata atgtttaaag ctaaaatgag aggttatgag gagataaaaa gttggatttt
      961 aagtatggga gcttatgttg aggttgtaga accagataga cttagaaatg atatattgtt
     1021 agagattgaa aaaatgaaaa aaatttactg attttttcaa ataatttata cttggaccaa
     1081 tatacttcac ggtctagtgt taatatatta attgagagag gtgatattat gaaaatgaaa
     1141 attgaattta gtacaggttg tataccaata tcttataatt cgctctttat gagtattata
     1201 aaagaagcaa taagaaaatc taatgaagat tactataaaa acatgtacta ttataaagaa
     1261 aagaataata aaaaaactaa aaattttaca ttttcggttt atataaaaaa gtatagtatt
     1321 gaaggtgaca attttattat agaagataaa gtaatattaa atataagtac tccagattta
     1381 gaattgggtt ttcatatata caatgggtta atgacttcta aaaaatgttt atataaagac
     1441 tatgaactta caagaattag aatcgattta tcaagagaaa agaaggtaac agaagaaagg
     1501 gtactattta atgcattgtc tccaatttgt gtaaagtcta aagaaggaaa attcttggag
     1561 ataactgatg atagatacat agaagaacta aactatataa ctaatgaagt ggttaaaaat
     1621 tataggggga atggattaaa aagaaagtta gaatttgaaa atataggtct caaaaaggta
     1681 gtagttaaag agagtttaag agaatttaaa aaaataacag gtaaagagta tcaatatatt
     1741 aatggttaca aaggtaaatt tatattgaaa ggtgatattg atgatttaaa tcttatctac
     1801 aatctaggaa ttggttttag acgtgctcag ggatttggag atgtagatat tttagaatgg
     1861 aggtgagata aatgaagtta agagtttata gaggagattg gttttttaat atgggtatag
     1921 ttggattttt aaatataata aagaaagcag ataaacaagc tgaaatattt attatggaag
     1981 attatatcga atttgatagc ttatttcttg aaaactttca tgagtattat tttaattact
     2041 ttatggatga atatgatgta tcaaaaagaa ttaaaaaaaa tattgattat agtataaatt
     2101 ttattaaatc taagcctgat agaatcaaag atggaattaa gaaaattaaa gatagtgtga
     2161 aacaacaaaa tgataagatt aaaaaatttg atgaagaaaa atttaacctg ataaaagaga
     2221 agctagatag tatgtctaag ataaaaagtt atgatgaatt tgacagttta gaagctttag
     2281 tagatgagac tatagatata tttaaaataa aatcaataaa tgacaggctt acagcaaatc
     2341 tttataaata tgtagttcaa gataattatt ttggacaagt tagttttttt aatgttgcta
     2401 aggcgaaatt agatttagat ggattaaaac aggtaatgtt taatgattat ttaagacaaa
     2461 ttatatattt tggagagtta gaagatttat tagaagagaa tgactatgat aagttgaaaa
     2521 actatttaaa tgatagatta aatagtatag ctaaggatat aaatgaaaaa aaagttagta
     2581 agtctagtat aaatactata gaaaaaatta tgaaggaaat aaataaaaac tttataaaga
     2641 aaaataaaag catagaagat ataaaaaagt atatggatag tttagaagta tgtgaaatgt
     2701 gtggtttata taagggaata ttagatgaat actcagaaag taattttgct ccacttggag
     2761 taagcaccaa taatgctagg aatatgtttt ggaatcaggc gtatgtacca tcaatatgtg
     2821 atatttgtaa gctaatattg ttctgtactc cagcaggggc tacttataca agaaagaatt
     2881 atttaattaa tgaagaaaat gagttttatt tatttgttaa catggatact tgtataaatg
     2941 agctttttga aagaaataat agtttaaaag ctcaaaaaag tgagtattca gattccaaag
     3001 aggaaaatcc ttttaatcag cttataaaaa gtatagtaga agaaaataca ttgaaaagtg
     3061 aatggcaact tcgtaatata ctttttgtgg aatttaaagc aagtatagat tctaaaaaat
     3121 gtaaaataaa ttattttaat attccaactt atttagcaaa attttttaca gataagtatg
     3181 caaataaaaa aatacaaagt atctatgatt acaagcttaa atcaaatgta ctagatttac
     3241 tcttgaaaaa tagggacttg aagcatctaa ttaataacat tttaagaaat aaagttaaaa
     3301 atgatatgga aagcaacaac aaatcaaata tatcaggaat agattgtttt agagtagtcc
     3361 aattaagagc attaataaat agctacaaaa agggggtata taaaatggat agtaaaaatt
     3421 tggagaagaa tgatgaaaag ctgagaataa tttattattt aggttgtgat atacatgact
     3481 attttgttaa taaaaattct aaaaataaaa taggtggagt aagctataaa cttttaaatt
     3541 cagttaaagt cggaaataaa agtgatttta tggatacaat tataagagtt tttatgtcag
     3601 ctgaaaaaca gatgccagca tttattttag atatagaaat tgaaaaagat ttagactttg
     3661 aatctatagg tcatgctttt atatctggat taatttctgg gaaatatgaa ggaaaagata
     3721 aactaccaaa cgaaaaggag gaaaaataag atgaaaagag ggttaacatt tacagtaata
     3781 gcacaagctc aaagtttaaa ctatggtgaa gggataggta atatatcaga attaaaaaaa
     3841 ttaagtagag atgatggaaa tatatataca tttgcatctc gccagtgttt aagatatgac
     3901 atagtacgat tagcagcaga attgtttaat tggaatttac aggttgtcga taaagaaaaa
     3961 ggtactattc aatttaaaga taatataagt ataagagaat cagaagaaat ggatttattt
     4021 ggatatatga aaacaaataa aaaagatgag aaagataaaa aaggaggttc tttgactaga
     4081 gaagctactg taagattaag taatgcgatt tctcttgagc catatagaag tgatatggat
     4141 tttttaaata ataaaggatt tgcagataga ataggagaac accctaatct tgctaacata
     4201 gagcaacatt taagttacta tacatataca gtgactatag atttatctaa aataggcaag
     4261 gatggagaga tagaacttga taataaagag aaatgtagaa gagtagtaga atttttagaa
     4321 ataataaaag tattgaatag aaatattaga ggtagacaag aaaatttatc tcctctattt
     4381 gtagtaggag gagtatatga aatagcaaat ccatttttct tgggtagaat taaattaaaa
     4441 ggagataaaa atggatttaa aataaataaa caagctatag aagaagtaat ccaaggtact
     4501 tttttaggaa aagatttaaa agaatttacc tatgttggaa tggtagatgg ggtatttata
     4561 aataaagaag aatttaaggg attgtttgaa gataattttt ttagtgtaga taaatttttt
     4621 ggacaactag ttaaagaagt aaaggaatat tatggggtga attaatatga aggttttgaa
     4681 attagattta tttcaagaaa ctgcatgcta caaaaaacca tttgctatga agattacaga
     4741 gacatatcca ctgcctccat attctacaat aaatggatta attcacaaaa tattaaatgc
     4801 tacagaatat ataccattta atataagtgt acagggaaca tatgaaagta tatttaataa
     4861 ttaccagaca acatattttt ataaaaaaga tagtattaca tctatgccaa tgaatagtca
     4921 tatgctattg aatgtaaatt tgataattca tataggtggg gattttgatt tattggaaga
     4981 aatatatgat agtttattaa attatgacga gcacttatca ttaggtagaa aagaagattt
     5041 agtaagaatt aatgatatta gatttgtaga agtcaataaa tttgaagtag atgaggatat
     5101 acaattagat gattatgaaa attataagtt atcagaatgt aatataagaa tgcctatata
     5161 tataccaaaa acaagtttac aagatacaga tatagatggt ataagttaca gactcaataa
     5221 ttactatgat aataatgctg tggaagataa aaaaagggtt tggaataagg tagatagtta
     5281 ttatgttgag gatggaaata ctatatcaat tggagatata ttattagata atgaaggaga
     5341 tttattatat tttaataata taaattagga gggagattat gttatatgct aaatctaatc
     5401 cagtggaaac acttagagaa catactgatg aacttttaaa acaaatgaat gtattaagag
     5461 aaagttatgg aaaaaatata aatagcttag attttctaga agaggagata ttttggcaat
     5521 tacttgacct tgttatagaa tttcatgata ttggaaaggc attttcaccg tttcaagagt
     5581 taataaaaag cagaatggat actaaaataa atgaacctaa aatagtaact catttagaaa
     5641 ataatgtagg ccataattat ctttctcctg catttattga ttatagttat atagacagga
     5701 aaaaaaataa agaacttaga gcagtattaa atcaagttat tgtatatcat catgaaagag
     5761 atatctttat agataaagat tttaaaaatt taatacaaaa aatattagat gaagatttaa
     5821 taaataaagt ttatgaacta cagcatgaat ttaaagttag atatccaata aaaacag
//
LOCUS       DAELLV010000215         5877 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.215, whole genome shotgun sequence.
ACCESSION   DAELLV010000215 DAELLV010000000
VERSION     DAELLV010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5877
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.215"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..1201)
                     /locus_tag="KPD58_003082"
     CDS             complement(<1..1201)
                     /locus_tag="KPD58_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0266813.1"
                     /translation="MKWKITRNFIFTIVFVAISVVIINIISILYVISTNSFFKVVDSG
                     NNPEEFARSFEKDLYEKDGEFKLSKIGAEKLEKSNSWIQVLNDLGEEVYGVNVPKDTP
                     KKYTPFQMVNNYKYIETKYVNFVLEKYLNKKHLNIIVGIPSRDISRIILTYSQNNIKK
                     TLNKVIIITLVIDSVVALGVGYLFSRKLTKPISSVLWSIETMANGNYSLYLKDRGIYE
                     EVFKNINMLADTLRVNEVERKENEELREEWLANITHDIKTPLASIQGYAEIINDKDYE
                     FEEDEIQEYTEIIYNKSKYIKDLVDDLNLSTRLKNNTIVLDKKKINLVSLVRNIIIDI
                     LNDNRYKNRNIEFESNEDLIEVYVDSILFRRAITNLIFNSIVHNSEGTLISVEIVKKD
                     NIEIIIKD"
     gene            complement(1205..1930)
                     /locus_tag="KPD58_003083"
     CDS             complement(1205..1930)
                     /locus_tag="KPD58_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0266814.1"
                     /translation="MENRVLIIDDEVEILKLLETVLKKEGLNNIYTAKTKKEGLELFK
                     SINPDLIVLDIMLPDGEGYDICKEIRKTSNSPIIFLSAKTEELDKLLGLAIGGDDYVT
                     KPFSPKEVAFRVKAHLRRLSYFSDAQNESKNLNNNEEKIISFGPYILNESRAELIKDG
                     KSIGLSAKELKILSLFAHNQNQIISKEKLWDKVWGEDYVGFDNTIMVHIRKIREKLED
                     NPSKPEYILTIKGLGYKLAVKED"
     gene            complement(2042..2815)
                     /locus_tag="KPD58_003084"
     CDS             complement(2042..2815)
                     /locus_tag="KPD58_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0266815.1"
                     /translation="MMYLVKAEMIKILKSKSVWIIWLFLIVFGFFLIRKFDVLDTYAD
                     IFYKIEGSIPLIGLIMFIITSGNYIKEYDSNMTGLINTTKKGKKSVVLSKLVANGLIL
                     SIINISFILLVGIRGFSFFDFKNLNESIHNLWYFGNSNLNLTVIQMYIIVILTTIFAS
                     FIFAQIGLTLSSIFKSAVIPFILGGLIMAIPYFSVGFIPDKAIKFMSVTPNWIMMSQQ
                     MVKYNVPSILIVFSIVISIILMIVLTKITYENFTSSKRF"
     gene            complement(2821..3708)
                     /locus_tag="KPD58_003085"
     CDS             complement(2821..3708)
                     /locus_tag="KPD58_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0266816.1"
                     /translation="MNKLEIENLSKIYGKKVANDKITVTLENGVYGLLGPNGAGKSTL
                     MKQITTLIKPDEGQILYNGEDIFKMDADYRNILGYLPQEFGVYKNFTAKEFLQYIGAL
                     KGLRGKYLNSKIGELLDLVGLYDVRNKSIGKFSGGMKRRVGIAQVLLNDPKIIVLDEP
                     TAGLDPQERARFRNLIAKMSRDKIIILSTHIISDIESVAKETIMIKDGKLLMKGTHKD
                     ILSHMENKVYNVQVKNECEVDDIQSKYKVVSIQSDIDSIVLRIVSEDIPSEDNIQPVQ
                     AKFEDVYMFYFDLEDAREV"
     gene            complement(3722..4744)
                     /locus_tag="KPD58_003086"
     CDS             complement(3722..4744)
                     /locus_tag="KPD58_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266817.1"
                     /translation="MVNWEIKKLAKSKSMFISLGILVLILMISIFIAPVLETESYYID
                     KEKGRIEDTRSGIDVGNEKFQNKINVLKQFSNQTDTGEFSKKIELMSKKKLDNLSVNE
                     YKDISFWKVFNYRVTNSLINVGMLIIISIIISNIYVDEVVTSVKDIILSSKEKKKALK
                     SKIFVALLVPLVIYSVYLMGIFIITCMQQGVPVNGDLESFRIVDNVVALKTNLSITKY
                     VILNIGIELLMFEGWAMIAIFGSFISKSSISSISFFIFIISITKIMSVIHILPKKLLS
                     VVSNVNYYDLIFGFNKIIGNYLGDIMIFKVNIDIVNIAIGLLMAILIGAMSLCFLVIK
                     SDYISR"
     gene            5319..5495
                     /locus_tag="KPD58_003087"
     CDS             5319..5495
                     /locus_tag="KPD58_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266818.1"
                     /translation="MFIGEIDKCTHILTAYISSSYDYCNFLDTQLDDFISEYGETVVE
                     TCLHQVLLLVSRYN"
BASE COUNT         2010 a          939 c          547 g         2381 t
ORIGIN      
        1 tatcttttat tataatttct atattatctt tttttacaat ttctacactt atcaaagttc
       61 cttcgctatt atgaactatt gaattaaata taagattggt tatagccctt ctgaataaga
      121 ttgaatctac atatacttca attaaatctt cattgctttc aaattcaata ttcctatttt
      181 tatatctatt atcatttaaa atatcaatta ttatatttct gactaaactg actaaattta
      241 tttttttctt gtccagaact atagtattat tttttaatcg agtagataaa tttaaatcat
      301 caactaaatc ttttatatac ttagacttat tatatataat ttctgtatac tcttgtattt
      361 catcttcttc aaactcataa tctttgtcat taattatctc tgcataccct tgtatagatg
      421 ctagaggtgt ctttatatca tgtgttatat ttgccagcca ttcctctctc aactcttcat
      481 tttcttttct ctcaacttca tttactctta aagtatcagc taacatattt atatttttaa
      541 aaacttcttc atatatacct ctgtccttaa gatataaact ataatttcca ttagccatag
      601 tctcaatact ccatagtacg cttgaaattg gttttgtaag ttttctacta aataaatatc
      661 ctacgcctaa agctaccacg ctatcaatta ctagtgttat aatgataact ttatttaaag
      721 tttttttaat gttattttga gaataggtta aaattattct tgatatatct ctactaggta
      781 tacctactat tatgtttaaa tgctttttgt ttaaatattt ttctagtaca aaattaacat
      841 actttgtttc tatatacttg taattattca ccatttgaaa gggtgtatat tttttgggag
      901 tatcctttgg cacatttact ccataaactt cctctcctaa atcatttaaa acctgtatcc
      961 aggaatttga tttttctaat ttttcagctc caatcttaga tagtttaaat tctccatctt
     1021 tttcatataa atctttttca aaagaacgtg caaattcttc aggattattt ccagaatcta
     1081 caactttaaa aaagctatta gttgaaatca catacaatat agatatgata tttataatta
     1141 ctactgatat agctacaaac acaattgtaa atataaaatt tctagtaatc ttccacttca
     1201 tattctaatc ttcctttact gctaatttat atccaagacc ttttattgtc agaatatatt
     1261 caggttttga aggattatcc tctaattttt ccctaatttt tcttatatga accattatag
     1321 tattgtcaaa tccaacataa tcttctcccc acactttatc ccatagtttt tctttactta
     1381 taatttgatt ttgattatgt gcaaataaag ataggatttt tagttccttt gcagacagtc
     1441 ctatagattt tccatctttt ataagttctg ccctactttc atttaatata tatgggccaa
     1501 atgatataat tttttcttca ttattattta gatttttact ttcgttttga gcatcactaa
     1561 aataacttaa tcttctcaaa tgcgctttaa ctctaaaagc cacttcctta gggctaaaag
     1621 gttttgtaac ataatcatct cctccaatcg ctagtccaag taacttatct aattcttcgg
     1681 ttttagctga taaaaatatt attggagaat tggaggtttt tcttatttct ttacaaatat
     1741 cataaccttc gccatctggt aacataatat ccaatacaat caaatcagga ttgatacttt
     1801 taaataattc taatccttct tttttagttt ttgcagtata tatattatta agaccctctt
     1861 tttttaggac agtttctaaa agcttcaata tttctacttc atcatctatt attaaaacac
     1921 gattttccat aaacaacctc ctgtaataat ttacatatat attagcatat attttatcta
     1981 aaattcattt atcttatttt taattaaact aaaatataaa taaaattact attattttta
     2041 gttaaaacct tttgcttgat gtaaaatttt catatgttat tttagttaac actatcatta
     2101 atataattga gattactata gaaaaaacta tcaaaatact tggtacatta tacttaacca
     2161 tttgttggct catcataatc caatttggag tcactgacat aaattttata gctttatctg
     2221 gtataaaacc tacactaaag tatggaattg ccattatcaa tccacctagt ataaatggaa
     2281 ttacagccga tttaaatata gatgaaagtg ttaagcctat ttgtgcaaat ataaaagaag
     2341 caaaaatagt agtcaatata acaattatat acatttgtat tactgtaaga tttaaattac
     2401 tatttccaaa gtaccataag ttatgaatcg attcatttaa atttttaaaa tcaaaaaaac
     2461 taaaacctct aattcctact aacaatataa aagaaatatt aataatagat aaaattaatc
     2521 cattagctac tagttttgat aatactactg atttttttcc ctttttagtt gtatttatta
     2581 aacctgtcat attagaatca tattctttaa tgtaatttcc agaagttatt ataaacatta
     2641 tcaaacctat tagaggtatt gagccttcta tcttgtaaaa tatatctgca taagtatcta
     2701 aaacatcaaa ctttctaatc agaaaaaatc caaaaactat taaaaacagc caaattatcc
     2761 aaacactctt tgattttaat atttttatca tttcagcttt aactaaatac atcatttcta
     2821 ctacacctcc cttgcatctt ctaaatcaaa ataaaacatg tatacatctt cgaattttgc
     2881 ttgaactggt tgtatattat cctcgcttgg aatatcctct gaaacaatcc taagtactat
     2941 tgaatcaata tcactttgaa tacttacaac cttatattta gattgtatat catcaacttc
     3001 acattcattt ttaacttgaa cattatatac tttattttcc atgtgactta atatgtcttt
     3061 gtgtgttcct ttcattagga gtttgccatc tttaatcatt attgtttcct ttgcaactga
     3121 ttctatatcg gaaattatat gagtagaaag tattattatc ttatctctag acatttttgc
     3181 tattaaattc ctaaatctag ctctttcttg tgggtcaaga cctgctgttg gctcatcaag
     3241 gactattatc ttagggtcgt ttagtaaaac ttgtgctatc ccaactctcc tcttcatacc
     3301 tcctgaaaat tttcctatag acttatttct cacatcataa agacctacta aatctaataa
     3361 ttctcctatc ttactattta aatattttcc ccttaaacct tttaaagctc caatatactg
     3421 taaaaattct tttgctgtaa aatttttata aactccaaat tcttgtggca aatatcctaa
     3481 aatatttcta taatcagcat ccattttaaa tatatcctca ccattataaa gtatctgacc
     3541 ttcatcaggt tttatcaacg ttgttatttg tttcattaat gtagattttc ctgctccatt
     3601 tggtcctaga agaccataaa ctccattttc tagagttact gtaatcttgt cgtttgctac
     3661 ttttttacca tatatctttg aaagattttc tatttctaat ttattcatct taactcccct
     3721 tttatctact tatataatct gattttatta ctaaaaaaca taatgacata gcacctatca
     3781 atattgccat caaaagtcct attgctatat tgacaatgtc tatattcact ttaaatatca
     3841 taatatcacc tagataattt cctattattt tattaaaacc aaatatcaaa tcataataat
     3901 ttacatttga tacaactgaa agtaattttt taggtaatat atggattacc gacattatct
     3961 tagttataga tataataaat ataaaaaaac ttattgaaga tattgaagac ttacttataa
     4021 aagacccaaa tatggctatc atggcccatc cttcaaacat caaaagctct atgcctatat
     4081 ttaatattac atactttgtt atacttaaat tagttttcaa tgcaactaca ttgtctacta
     4141 ttctaaaaga ttctaagtct ccattaacag gtactccttg ttgcatacaa gttataataa
     4201 atattcccat taaataaaca gaataaatta ctaatggaac taataaagca acaaatattt
     4261 tagactttaa tgcttttttc ttctcttttg atgatagtat gatatctttt accgaagtaa
     4321 cgacttcatc tacgtaaata ttggatataa ttatagatat aatgattaac attcctacat
     4381 ttataagtga atttgtaact ctatagttaa atactttcca aaaactaata tccttatatt
     4441 cattcacact tagattatca agttttttct ttgacattaa ttcaattttt ttagaaaatt
     4501 cacctgtatc tgtttgattt gaaaactgct ttaaaacatt aatcttattt tgaaatttct
     4561 cattacctac gtcgatacca cttctcgtat cttctatacg tcctttttct ttatctatat
     4621 aataactttc tgtttctaaa acaggcgcaa taaagatact aatcattagt attaacacta
     4681 atatccctag agatataaac atactcttag atttagccaa tttttttatt tcccaattta
     4741 ccaatatatt tctcctccta aatcatttta attacacata tatattaaaa cttaaatctt
     4801 atataaatat aatactaacc ttaaataaag cttaaattat tttataattt ataggtgtgt
     4861 ttattattgg agttacattt attatttttc tgggaaatta tacattttca attattttta
     4921 ttatttttga tatttttatt gattggaaat tttattgttg ttcaaattgt agttattctt
     4981 taaatttaaa gatggagttt tttaagaata gaacctactg tccttattat ggaaagatta
     5041 ttaaatagtt gcatataact ttatttatct tgaatcttat ttttaaaaac aattataatt
     5101 ttagaatatt ttctattctc aaacaagcat gttgaataat ggagcatctt ctagtattat
     5161 atacttgtag aataaaacta aattggtaaa actgggaata tttaacctta aggagttaag
     5221 ttatagaaac tagtactaaa aaattttata aattatgtta attgactaat agaatgcgac
     5281 tttttatttt tactaaattt tttaggggga tggataatat gtttattgga gaaatagaca
     5341 aatgtacaca tatactaact gcttacatca gtagttcata tgattattgt aattttttag
     5401 atacacagtt agatgatttt atatcagaat atggagaaac ggtagttgaa acctgcttac
     5461 atcaagtctt attattagta agtagataca attaaaacta aatttttcta gaaaaacctt
     5521 tttagattta tctactctaa taaatatata attttttgta ctttataagt aactataact
     5581 tctgctaaag taaagatgta ttgggagtac atctttactt tcccattata gaagttaaaa
     5641 gcatacacca taaaatcaat catccaatac aaattatata tttcctatgt ctaacttaat
     5701 ttacaagaat cattctatat gcatattctg caatccacta aacatggctc taaaccttta
     5761 ctattttata cattctctat aaatcataca aatcatatat aaaatccccc taatatgttt
     5821 aaaccaccta taaaataaca ccaatattta gaatattcaa tatcaactgc ataactg
//
LOCUS       DAELLV010000216         5703 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.216, whole genome shotgun sequence.
ACCESSION   DAELLV010000216 DAELLV010000000
VERSION     DAELLV010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5703
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.216"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            113..1120
                     /gene="asrA"
                     /locus_tag="KPD58_003088"
     CDS             113..1120
                     /gene="asrA"
                     /locus_tag="KPD58_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrA"
                     /protein_id="HBG0266819.1"
                     /translation="MKLKLDSSKFNEGLNYLKKDYKIFAPVLIPFKGTFSDTDVIRYK
                     EVNAFEEMEFAKKANFSPKEVVLPINQVLFYFTEKEFKESDLDNKKILIFLRACDING
                     IKRLDEIYLNNGEEKDYFYKNIRDKVKFALVGCKESFRNCFCVSMDSNKTDNYSIGLN
                     IEGDTLYLDIKDSEFEVFNGEATEFNVDYVTDNLISVDVPENIDSNEMIGNSIWDEYD
                     TRCIGCGRCNFVCPTCSCFTMQDIFYKENENVGERRRVWASCQVDGYTDIAGGNSFRK
                     KQGERMRFKTMHKIHDFKKRFGYHMCVGCGRCDDACPQYISFSKCIEKINDLVISKEE
                     V"
     gene            1121..1912
                     /gene="asrB"
                     /locus_tag="KPD58_003089"
     CDS             1121..1912
                     /gene="asrB"
                     /locus_tag="KPD58_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HBG0266820.1"
                     /translation="MNPYIPVSAKILDIVKHTDKEWTFRVNCNTKGVLPGKFYEISIP
                     KYGESPISVSGYGEDYIDFTIRNVGKVTSELFNYKEGDSFFIRGPYGNGFDVSLYEGR
                     EIVVVAGGSALAPVRGIVEYFYNNKEKCEKFKLIVGFKSPKDILFADDLKRWSKKLDI
                     LVTVDGAEEGYSGNVGLVTKYIPELDMKDINNTSIVVVGPPMMMKFTVEEFLKRDLDE
                     KNIWVSYERKMCCGIGKCGHCKMDDTYICLDGPVFDYSYAKNLID"
     gene            1925..2896
                     /gene="asrC"
                     /locus_tag="KPD58_003090"
     CDS             1925..2896
                     /gene="asrC"
                     /locus_tag="KPD58_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HBG0266821.1"
                     /translation="MIRDLNTKKVMKNAYRITKTKYETSLRVRVPGGLIDPESLAVVS
                     KIASEYGNGQVHITTRQGFEILGINMEDMEEINKIIQPVIEKMNINQSEKNAGYPAAG
                     TRNIAACIGNKVCPKAQYNTTEFAKRIEKAIFPNDLHFKVALTGCPNDCIKARMNDFG
                     IIGMALPIYEKDRCVNCGACVKKCSKISVGALKTENNKVVRDKDKCIGCGECVLNCPT
                     NAWTRDEKKYYRLAIMGRTGKKNPRLAEDFLLWVDEDSIIKIILNTYRYVEKYIDKDA
                     PGGKEHIGYIIDRTGFMEFKKWALEGVELPEITKMYENIYWSGIKYL"
     gene            2953..3729
                     /locus_tag="KPD58_003091"
     CDS             2953..3729
                     /locus_tag="KPD58_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBG0266822.1"
                     /translation="MHKETLDKLTNAAINKINLLNTSKVKYLVSSAFAGLYVGIGILL
                     IFTIGGLLTDAGSPMTKIVMGLSFAIALSLVIMTGTELFTGNNMVMSAGMLNKGVSIK
                     DTSKIWAYSWVGNLIGALVLGIIFVGTGLVDKGPVAEFFANTAASKASMPFTALFFRG
                     ILCNILVCVSVLCSFRTNSDTAKIIMIFLCLFAFITSGFEHSVANMTIYSVSLFSPTI
                     STVTIGGAIYNLVAVTLGNIVGGALFMGLGTYILGKEKLN"
     gene            4000..>5703
                     /locus_tag="KPD58_003092"
     CDS             4000..>5703
                     /locus_tag="KPD58_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBG0266823.1"
                     /translation="MTKKLVISKTKLFIFIIAFISIFIYLFGSKNTLIGVGIVTAMLT
                     LLERDLTISPIKNLLKYLAINIILGILSFFAVQNMYLGVLLNFIALFIIGYVFSYDLK
                     RAVYVPFGLMYIFMVSIPVGKSEFPMRLSALAVGAVIIMIAQFVMNRNRMKNVGDKEL
                     ISICDELLEKISLLKNTINDDSSIIKSSMRKIDSCNYRINSISKNLKMVIFDNRKDDF
                     YISIRGIDIMNILFSLERISLILERYKKDSKEFEDEDIKNILEESSINSKSDVLVVAT
                     KEINYIKMCLENKDTITDKEILGFRDYVISQDTKNINLKEIYSVLENLYEFLVDYKKV
                     KSRDEKKAERKSKIPHEFKRLSIYKKNFNLNSIRFSYAVKIALATAVAGFIMDYFHLR
                     DGRWIMLTVFSLTQPYAENCIQRSRKRIEGTFIGAVIFIVLFSIIKDSTLRSLIVLLA
                     GYINSYVVDYRKLMVCVTVSALGSAVVMGDPNVLTISRISYVALGAIIALIVNKFILP
                     YDAKTGYQHVIEMYKGIVKNIIDEVNKSIENVADVYYIKNLLLIPSLIEDRLMLINAI
                     YK"
BASE COUNT         2265 a          511 c         1066 g         1861 t
ORIGIN      
        1 ttttattatt tttgaaactg tgataattat cacggaagca tagaaaataa agtgttaaga
       61 tttaaacaag atagcaagta agtaaaacta aaatataaga aaggtgataa aaatgaaact
      121 aaaattagat tcatctaagt tcaatgaagg tctgaattat ctaaaaaaag actacaagat
      181 atttgcacca gttttaatcc catttaaagg tactttttca gatactgatg ttataagata
      241 caaggaggta aacgcttttg aagaaatgga atttgctaag aaagcaaact tttcaccaaa
      301 agaagttgta cttcccataa atcaagtatt gttttatttt actgaaaaag agtttaagga
      361 aagcgattta gacaacaaga aaattttaat tttcttaaga gcttgtgaca taaatggtat
      421 aaaaagactt gatgaaatat atctaaataa tggtgaagaa aaagattatt tttataaaaa
      481 tatcagagat aaagtaaagt ttgcacttgt agggtgtaag gaaagtttta gaaactgttt
      541 ttgtgtgagc atggattcta acaaaactga taattattca attggattaa atatagaagg
      601 agatacactg tacttagata taaaggatag tgaatttgaa gtatttaatg gagaagcaac
      661 tgaatttaat gttgattatg taactgataa tttaatatct gtagatgtgc cagagaatat
      721 tgattcaaat gaaatgatag gaaattcaat atgggatgaa tatgatacaa gatgtatagg
      781 atgtggtaga tgtaattttg tatgtccaac atgttcatgt tttacaatgc aggatatttt
      841 ctataaggaa aacgaaaatg taggtgagag aagaagggtt tgggcttctt gtcaagtaga
      901 tggatataca gatatagcag gtggaaattc ttttagaaaa aaacaaggtg aaagaatgag
      961 atttaaaact atgcataaaa tacatgattt taaaaaaaga ttcggatatc atatgtgtgt
     1021 tggttgtgga agatgtgatg atgcctgtcc gcaatacata tcattttcaa agtgtataga
     1081 aaaaataaat gatttagtaa ttagtaagga ggaagtataa atgaacccat atataccagt
     1141 aagtgcaaaa atactagata tagttaaaca tactgataaa gaatggactt ttagagtaaa
     1201 ctgcaataca aaaggtgtat tgcctggaaa gttttatgag atatctatac ctaaatacgg
     1261 tgaaagtcca atatcagtat caggatatgg agaggattat attgacttta caataagaaa
     1321 tgtaggaaaa gtaactagcg agttgtttaa ttataaagaa ggagattctt tttttataag
     1381 aggtccttat ggaaatggat ttgatgtatc cctttatgaa ggaagagaaa ttgtagtagt
     1441 ggcaggagga agtgcattag cccctgtaag aggaatagtt gaatattttt ataataataa
     1501 agaaaaatgc gaaaagttta aattaatagt aggatttaaa tcaccaaaag atattttatt
     1561 tgcagatgat ttaaagagat ggagtaaaaa acttgatatt ttagttactg tagatggagc
     1621 agaggaagga tatagtggta atgtaggatt agtaacaaaa tacataccag aattggatat
     1681 gaaagatata aacaatactt ctatagttgt agttggacct ccaatgatga tgaaatttac
     1741 agtagaagaa tttttaaaaa gagatttaga tgaaaagaac atttgggttt cttatgaaag
     1801 aaaaatgtgt tgtggaatag gtaaatgtgg acattgtaaa atggatgata cttacatatg
     1861 tttagatgga ccagtatttg attattcata tgctaaaaat cttattgatt agggggtgaa
     1921 gtttgtgata agagatttaa ataccaaaaa agtaatgaaa aatgcttata gaattacaaa
     1981 aacaaaatat gagacttctt taagagttag agtacctgga ggtcttattg accctgagag
     2041 tttagctgta gtttctaaaa ttgcaagtga gtacggcaat ggacaggtgc acataactac
     2101 aagacagggg tttgaaatat taggaataaa tatggaagat atggaagaaa taaataaaat
     2161 tatacaacct gttattgaaa aaatgaatat aaatcaaagt gagaaaaacg caggttatcc
     2221 agcagcaggt acaagaaata tagcagcttg tataggaaat aaagtctgtc caaaggctca
     2281 atataataca actgaatttg ctaaaagaat agaaaaagct atatttccaa atgatttaca
     2341 ttttaaagtg gctttaactg gttgtccaaa tgattgtata aaagctagaa tgaatgactt
     2401 tggaattatt ggtatggctt taccaatata tgaaaaggat agatgtgtaa attgtggagc
     2461 atgtgtaaaa aaatgttcta agatatctgt tggagcatta aaaacagaaa ataataaggt
     2521 ggtaagagat aaagataagt gtataggctg tggagagtgt gtactaaatt gcccaacaaa
     2581 tgcatggact agagatgaga aaaaatacta tagattagct ataatgggta gaacaggaaa
     2641 gaaaaaccct agactagcag aagattttct tttatgggta gatgaggaca gcataataaa
     2701 gataatactt aatacttata gatatgtaga aaagtatata gataaagatg caccaggagg
     2761 aaaagaacat ataggatata taattgatag aacaggattt atggaattta aaaaatgggc
     2821 acttgaaggt gtagagcttc cagaaataac taaaatgtac gaaaacatat actggagtgg
     2881 aattaaatat ctataaattg agtaaaacat atatttgaat aaattaaaat ttaatagaag
     2941 gaagagggag ttatgcataa agaaactttg gataaattaa ctaatgcagc tataaataag
     3001 ataaatttat taaacacaag taaagtaaaa tatttggtat cttctgcgtt tgcaggactt
     3061 tatgtaggta taggtatact tttgatattt actattggag gacttttgac tgatgcaggt
     3121 tcacctatga ctaagatagt aatggggtta tcatttgcaa tagcactaag tttggttata
     3181 atgacgggaa cagagttatt tactggaaac aatatggtta tgtctgctgg tatgttaaat
     3241 aaaggtgtaa gtataaaaga tacttctaaa atatgggcat acagttgggt aggtaattta
     3301 atcggagctt tggttttagg cattatattt gtaggaactg gtctagtaga caaaggacct
     3361 gtagctgaat tttttgcaaa tacagcagct agtaaagcat ccatgccatt tacagctctt
     3421 ttctttagag ggattttatg taatattcta gtatgtgtat cagtgttgtg ttcgtttaga
     3481 actaatagtg atactgcaaa aataattatg atattcttat gtttatttgc ttttataaca
     3541 tcaggttttg agcatagtgt agcaaatatg acaatttata gtgtttcatt attttcacca
     3601 acaataagta cagttactat tggaggagct atttataatt tagtagctgt aacacttgga
     3661 aatattgttg gaggagcttt atttatgggt cttggaactt atatattagg taaagaaaag
     3721 cttaattaaa tttattgtat aaattataac tctcttgata aatacatttt tagtattgtt
     3781 gagagagtta tttttatatt tttgaaaaaa tatttgaaac aaaaaaaata tgggagcata
     3841 cgaaaatata tcggttatat atataaaaaa tacgtttata tatgaattat atagttctaa
     3901 atataaaaaa agttaaagtc ctaccgtaaa aaagcgcatt ttgaataaga taacaaatat
     3961 gataaaattg gtcttgttga aatttttagg ggggatatga tgacaaagaa actagtaata
     4021 tcaaaaacaa agttgtttat ttttataata gcatttattt ctatatttat atatttattt
     4081 ggaagtaaaa atacacttat aggtgtaggt atagtaacag ctatgctaac actcttggaa
     4141 agagatttaa ctatttctcc aattaaaaat ttactgaagt atttagcaat aaatataatc
     4201 ttaggaatac tatcattttt tgcagtacaa aatatgtatt taggagtttt attaaatttt
     4261 atagcattat ttataatagg gtatgtattt agttatgact taaaaagagc agtatatgta
     4321 ccctttggtc taatgtatat atttatggta agtatacctg taggaaaaag tgaatttcct
     4381 atgagattgt cggctttagc tgttggagca gttataataa tgattgctca atttgttatg
     4441 aatagaaata ggatgaaaaa tgtcggagat aaagaactga tatctatatg tgatgaatta
     4501 ttagaaaaaa ttagtctttt aaaaaatact attaatgatg atagttctat aattaaatct
     4561 agtatgagaa aaatagatag ctgtaattat agaataaatt ctatatctaa aaatttgaaa
     4621 atggttattt ttgataatag aaaagatgac ttttatatca gtattagagg tatagacata
     4681 atgaatattt tattttcatt agaaagaata agtttaatcc tggaaagata caaaaaggat
     4741 agtaaagaat ttgaagatga agatataaaa aatattttag aagaaagtag cataaatagt
     4801 aaatcagatg ttttagttgt agcaacgaaa gaaataaact atattaagat gtgtttagaa
     4861 aataaagata ccataactga taaagagata ttaggattta gagattatgt tatttctcag
     4921 gatactaaaa atataaattt aaaagaaatt tatagtgttt tagaaaattt atatgagttt
     4981 ttagtagatt ataaaaaagt aaaatcaaga gacgaaaaga aagctgaaag aaaatcaaag
     5041 atacctcatg aatttaaacg actatcaata tataagaaaa actttaattt aaattctata
     5101 agattttctt atgcagtaaa gattgcttta gctacagctg tagcgggttt tattatggat
     5161 tatttccatt taagagatgg aagatggata atgttaactg tcttttcatt aacacaacct
     5221 tacgcagaaa attgtataca aaggtctaga aaaagaatag aaggtacatt tataggagct
     5281 gtaatattta ttgtattatt ttcaataata aaagatagta cattaaggtc gttaatagtt
     5341 cttttagcag ggtatataaa ctcttatgta gttgattata gaaaattaat ggtatgtgta
     5401 actgtttcag ctcttggttc agcagtagtt atgggagacc caaatgtttt aacaataagc
     5461 aggatttcat atgtagcatt aggagcaatt attgcattaa tagtaaataa gtttatatta
     5521 ccatatgatg ctaaaacagg ctatcaacat gttatagaaa tgtataaagg aatagttaaa
     5581 aatataattg atgaagtcaa taagtctata gaaaatgtag ctgatgttta ctatataaaa
     5641 aatttattat tgataccatc acttattgaa gatagactta tgttgataaa tgctatatat
     5701 aaa
//
LOCUS       DAELLV010000217         5667 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.217, whole genome shotgun sequence.
ACCESSION   DAELLV010000217 DAELLV010000000
VERSION     DAELLV010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5667
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.217"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..377)
                     /locus_tag="KPD58_003093"
     CDS             complement(<1..377)
                     /locus_tag="KPD58_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBG0266824.1"
                     /translation="MLVSRQIEFLKLILNENKYRPIKYFKDKLNVSDKTLQKDLKMIE
                     KYLETFNIKIDIKRGYGILIEDLAKKNIDLINSLNIQSKENKSLSVEQRRMEILKYLL
                     LNSNNITSINQLADKYYVSKTSIV"
     gene            complement(645..2540)
                     /locus_tag="KPD58_003094"
     CDS             complement(645..2540)
                     /locus_tag="KPD58_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0266825.1"
                     /translation="MIGKILRSDCIVLDKQLQTKKDVINYMANHLYHGNYINNKEKFI
                     ADINKREKESSTNMGNLIAIPHAKSNVVNQPVMMYLRTDREITDNDGQKLRSFFMIAV
                     PESGSDEHLKIISKLATYLMEENFLKHLLSAETPYEILSIFNNKAAESGDFSNNQNKG
                     LIVGITGCPTGIAHTFMAKKALEEAGQELGYEVKIETHGAIGVENKLTKEDIKRAEYV
                     IIACDKEVQLSRFIGKKVLQKKVVDAKNVSLAKNLIINAENGMAKEIKPGGKTLEDSK
                     EMLQGSEKKGVYKHLMSGVSEMIPFVVIGGIGIAIAFMFGIFASDPTHETYNPIAGFF
                     GQLGGDSAFKLFIPILAGFIAKSIAGRQGFAPAMIAGFMAVIGGSGFIGGMVAGFATG
                     YIALGVMKITENLPDVLKGVNAVLICPLLIAFTSGAIMFFVVNTPASWLNEALQSWLQ
                     SMSGTNKVLLGAILAGMMASDMGGPINKTASAFGLAMFSSNIFEPSAALMVGGMVPPL
                     AISLAMLIFKNKFTKEERKSSLPTAIMGASFITEGAIPFAAADPLRMIPANIIGSCIG
                     GAICMSLDITLMAPHGGIFVIPFACSAPVAYIACILIGTVITALIIGVTKPTIRSENS
                     EVELLGA"
     gene            complement(2576..3454)
                     /locus_tag="KPD58_003095"
     CDS             complement(2576..3454)
                     /locus_tag="KPD58_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBG0266826.1"
                     /translation="MLINMKEMLKVAQENQFAVPAFNIGSGQILKAVVQSANEKNAPV
                     ILAIHPNELSFLGDSFVASCIEEANKSKVPMVIHLDHGENKEQILRAIRCGFTSVMID
                     GSHLPYEENVAISREIVEIAKGLNVSVEGELGTIGTTGTSSEGGTDEIIYTDSKLAKD
                     FVEKTGVETLAIAIGTAHGIYPKGFKPELKLDLLKEIREVVDIPLVLHGGSSNPDEEI
                     AQAVKLGVCKINISSDVKSAYYKKCRELLEQNPSLYEPDTIYPPCIKSAREVIEFKMN
                     LFNAIDKLKCFYKNKN"
     gene            complement(3997..4167)
                     /locus_tag="KPD58_003096"
     CDS             complement(3997..4167)
                     /locus_tag="KPD58_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266827.1"
                     /translation="MSALLYGVCNKSISVSIVINMQEFKIGVSMAYGVLVIGICYIAL
                     GIIFKLDRKRFT"
     gene            complement(4564..4776)
                     /locus_tag="KPD58_003097"
     CDS             complement(4564..4776)
                     /locus_tag="KPD58_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0266828.1"
                     /translation="MAILMKTKIKEYREKLLMTQNELAKLVGVRRETIVHLENGKYNP
                     SLKLAMDIAKVFDTTVENLFEFIEEE"
     gene            complement(4777..5193)
                     /locus_tag="KPD58_003098"
     CDS             complement(4777..5193)
                     /locus_tag="KPD58_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266829.1"
                     /translation="MSKSETGVRSKLILELLVITYFFIAVLATKYIFKGPSTASLPGL
                     SWLIVFGCYSIYNKKYNDLVDESSEFILSKVNKIAIKIMIYSIVIISIFFATPYTSNI
                     NISNLDTGIILIAVLFIQSLSKLVLYIYFDKRGICN"
     gene            complement(5261..>5667)
                     /locus_tag="KPD58_003099"
     CDS             complement(5261..>5667)
                     /locus_tag="KPD58_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266830.1"
                     /translation="SELGVKTNLVSELLMISYFFIDSLLKKFIFKEGNLCITFIILFA
                     FWACDFMYNKKYNDLVDEASKLMLLKVDHIASKIMLYSILLIAVFFSANYTRGLDISN
                     LDIGVILLAILFIQSLSKLILYIHFDRKGICN"
BASE COUNT         1855 a          986 c          596 g         2230 t
ORIGIN      
        1 actatagaag ttttacttac ataatattta tctgctaact gatttattga agttatatta
       61 tttgaattta gtaataaata ctttaaaatc tccattcttc tctgctctac tgataaagat
      121 ttattttcct tcgattgtat atttaaacta ttaattaagt ctatattctt ctttgctaaa
      181 tcttctatta atattccata gcctcttttt atatcaattt ttatattaaa agtttctaaa
      241 tatttctcaa tcatcttcaa atctttttgt aaagttttat cagatacatt taatttatct
      301 ttaaagtatt ttataggtct atatttattt tcgtttagta tcaatttaag aaattcaatt
      361 tgcctcgaca caagcatact tagccctccc tacctatgtt tatatatttc tatgtttata
      421 ttaagtacta ttttaacata tttactaact atgtaaaatt actaagatac ttctgtatca
      481 taaaagaatt tatgatacac cttactaaat tgatataata gataatataa tttcaaaata
      541 aaaattaagg atatttagaa gtagttaact gattctaaat atccttatta ttattctata
      601 tacttgttgt tattccatta attattttta ctattattta taatttaagc accaagtaat
      661 tcaacttcac tattttcact ccttatagta ggctttgtta cccctataat caatgctgtt
      721 attactgtac caatcaatat acaagctata tatgcaactg gtgcactaca tgcaaatggt
      781 attacaaata ttcccccatg tggtgccatt aaagttatat ctaaactcat acatattgcc
      841 ccacctatac aagaaccaat tatatttgct ggaatcattc ttaatgggtc tgcagctgca
      901 aacggtattg cgccttctgt tatgaaagat gcccccatta tagcagttgg tagagatgac
      961 tttctttctt ctttagtaaa cttatttttg aatattaaca tagctaatga tattgctaat
     1021 ggaggtacca ttcctcctac cattaatgct gctgatggtt caaatatatt acttgaaaac
     1081 atagccaatc caaaagctga agctgtttta ttaataggtc ctcccatatc tgatgccatc
     1141 atccctgcta atattgcacc taataatact ttattagttc cagacatact ttgtaaccaa
     1201 gattgtaaag cttcatttaa ccatgatgct ggcgtattaa ctacaaagaa cattatagct
     1261 ccactcgtaa atgcaatcaa aagtggacat atcaatacag catttactcc ttttaataca
     1321 tctggtaaat tttctgttat cttcattact cctaaagcta tatatcctgt tgcaaaacca
     1381 gctaccatac cacctataaa acctgaacct ccaataactg ccataaaacc agctatcata
     1441 gcaggagcaa atccttgtct tcctgctatt gatttagcta taaaaccagc aagtatcggt
     1501 ataaataact taaatgctga atcgcctcca agttgtccaa agaatccagc tatagggttg
     1561 tatgtttcat gagttgggtc tgatgcaaat ataccaaaca taaaagctat ggctatacca
     1621 attccaccta tcactacaaa tggaatcatt tcagatacac cactcattaa gtgcttataa
     1681 acaccctttt tctctgaccc ttgaagcatt tcttttgaat cttctaaagt ctttccacct
     1741 ggtttaattt ctttagccat tccattttca gcgtttataa ttaaattttt agctaatgaa
     1801 acattcttag catctactac tttcttttgt agcactttct ttcctataaa tctactcagt
     1861 tgaacttctt tatcacaagc tattattacg tattcagctc tttttatatc ttctttagtc
     1921 aacttatttt ctacacctat agctccatga gtttctattt taacttcata tccaagttct
     1981 tgacctgctt cctctaatgc tttttttgcc ataaaagtat gagctatacc tgttggacat
     2041 cctgttatcc caactataag acctttattt tgattattac taaaatctcc tgattctgca
     2101 gctttattat taaatatact taatatttcg tatggagttt ctgcacttag tagatgctta
     2161 agaaaatttt cttccattag atatgtagct agtttagata taatcttaag atgttcatct
     2221 gaacctgatt ctggaactgc tatcataaaa aatgacctaa gtttttgacc atcattatct
     2281 gtaatctctc tatctgttct taaatacatc attacaggtt gatttactac atttgactta
     2341 gcatgaggta tagctattaa attacccata ttagttgatg attctttttc gcgtttatta
     2401 atatcagcta taaacttttc tttattattt atataatttc catggtacaa atgatttgcc
     2461 atataattaa ttacatcttt ttttgtttgt aattgtttat ctagtacaat acaatcgctt
     2521 cgtagtattt tacctatcat aaattctccc ctccttttaa tttgttacct cataattaat
     2581 ttttattttt ataaaagcac tttaacttat ctatagcatt gaatagattc attttaaact
     2641 ctataacttc tcttgcagat tttatacatg gtggatatat agtatctggc tcatataagc
     2701 ttgggttttg ttctaatagt tctctgcact tcttataata tgccgactta acatcactcg
     2761 aaatatttat tttacaaact cctagtttaa ctgcttgtgc tatttcttca tctggatttg
     2821 atgaaccacc atgaagaact aaaggtatat ctactacttc tcttatttct tttaataaat
     2881 ctagtttaag ctctggttta aaaccttttg gatatatacc atgtgctgta ccaattgcaa
     2941 tagctagtgt ttctactcct gttttttcaa caaaatcctt agctaatttt gaatctgtat
     3001 atattatttc atcagtacca ccttcacttg atgttcctgt tgttcctatt gttccaagtt
     3061 ctccttctac ggacacattt aatccttttg ctatttctac tatttcccta gatatagcta
     3121 cattctcttc atatggtaaa tgtgagccat caatcattac tgatgtaaat ccacatctta
     3181 ttgcccttaa tatttgttct ttattttccc catgatctaa gtgtattacc ataggtactt
     3241 tactcttatt tgcttcttct atgcaactag ctacaaagct atctcctaga aaacttagct
     3301 catttgggtg tattgcaagt attactggtg catttttttc gttagcactc tgtactactg
     3361 ctttaagtat ttgcccactt cctatattaa atgctggtac agcaaattga ttttcttgtg
     3421 ccacttttag catttccttc atgtttatta acatattgta cctcccgata ttttaaattt
     3481 taggttgaat ttgcttcttg ttaattttgt tgttgtttat atttaaataa tatcagggta
     3541 atgaatgtca gaaaataaca atcgttttcc ttagtgaaaa ggaactttta ttatacactc
     3601 taagttcctt ttcactaaat aatttttaga agtctgcatc atagttaatt atatttctca
     3661 tgtacatctt ctctttttaa gtgttttatg ctttagcctt cctagatttt ataataaaca
     3721 attaagatac attaactata aatttaaaga tactcatgca atagttaatg ctatattaga
     3781 gtcaaaaaaa ttaaaaagat attagctatt tggccaatac cttttcttgg attccaatag
     3841 tatatacgat ttatagaaat cttactcacg attatctaat gttttacttc cataaacttt
     3901 acataaaaca ccaaagtgac accctccaaa ttcatctcta gctaataaag tgatttggtt
     3961 ttctaagcac caatttataa attagccttt gttatcttat gtaaatcttt ttctatctaa
     4021 tttaaaaatt attcctaatg ctatatagca tataccaatt actaatacac cataagccat
     4081 agatactcct atcttaaatt cttgcatatt aattacaata gaaactgaaa tagatttatt
     4141 acaaacacca taaagcaagg ctgacattgt atattatcca attgtatgaa tgaatactaa
     4201 tatagctcct tttaattaaa tgatgctata taaatcatat ttatcccaat ttttaatcta
     4261 acctatttat atgtatattc ctttttaata aaaaaagata ttgactaaat agtcaatatc
     4321 ttttagttga tttaaatacc cacaatttta atctaatttt taagtgtttc cttaacataa
     4381 tcttataaat attaaaattt atctacctat aaaatttctc cattaaattt tattacattt
     4441 tatcttaaaa caattataat ctgaccttaa tcatatatta tattatataa atatttttaa
     4501 caaacttaat aattaaataa ctatatttta ttatccatct tataaatgtt tataaacttt
     4561 ccactattct tcttcaataa attcaaacaa attttctact gtagtatcga atactttagc
     4621 gatatccata gctaacttca aagaaggatt atatttccca ttttctaagt gtacaatagt
     4681 ctcacgtctt accccaacca atttagcaag ttcattttgc gtcataagga gtttttctct
     4741 gtattcttta atttttgttt tcatcaatat tgccacttag ttacatatcc cccttttatc
     4801 aaagtatata tacaacacta atttagataa agactgtata aataaaactg caatgaggat
     4861 tatacctgta tctaggtttg atatatttat attacttgta taaggtgttg caaagaaaat
     4921 agatattatt actatagaat atatcattat ttttattgca atcttattaa cctttgatag
     4981 tataaattca gatgattcat caactaaatc attatatttt ttattataaa ttgaataaca
     5041 tccaaaaact attaaccaac tcaaccctgg aagagaagca gtgcttggac ctttaaaaat
     5101 atacttagtg gctaatacag ctataaaaaa ataagttatt acaagcaact ccaggataag
     5161 ctttgatctt acaccagttt ctgatttaga catataaaat cactccttaa aaattatatt
     5221 ctctatactc tttaattttc gtttttatca atattatcac ttagttgcat attccctttc
     5281 tatcaaaatg tatatacaaa attaatttag ataaagattg tataaataaa atagctaaga
     5341 gaattacacc tatatccaaa tttgatatat ccaaacctct tgtataattt gctgaaaaga
     5401 aaacagctat tagcaaaata gaatataaca ttatttttga tgcaatatga tcaaccttta
     5461 ataacataag ttttgacgcc tcgtcaacta aatcattata ttttttatta tacatgaagt
     5521 cacatgccca aaaagcgaat aaaattataa aagtaataca aagatttccc tctttaaaaa
     5581 taaacttttt taataatgag tctataaaaa aataggatat cataagtaac tctgaaacaa
     5641 gatttgtttt aacaccaagc tctgatt
//
LOCUS       DAELLV010000218         5636 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.218, whole genome shotgun sequence.
ACCESSION   DAELLV010000218 DAELLV010000000
VERSION     DAELLV010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5636
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.218"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            145..774
                     /locus_tag="KPD58_003100"
     CDS             145..774
                     /locus_tag="KPD58_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0266831.1"
                     /translation="MDKNYTMPIYQKIALDIANKIYTGEIQEDSVLFGRSVLAGKYNV
                     SPETIRRAVKILEDIGVVKSIKGKGVIVLSPDKASSFIKKYRDITNISSYKSTLYNLI
                     DTKSNLENEILDTINKILDYSNRLEIINPLVPVQFTINSNCKYIGQTAAQTKFWQNTG
                     ATIVAIKRGEELIISPGPYIEFLEGDILLVVGDQHIYNSIPMFLYENEK"
     gene            complement(888..2402)
                     /gene="glpK"
                     /locus_tag="KPD58_003101"
     CDS             complement(888..2402)
                     /gene="glpK"
                     /locus_tag="KPD58_003101"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBG0266832.1"
                     /translation="MEKKYVMALDQGTTSSRAILFNKKGEIVKIAQKEFNQIYPKAGW
                     VEHDPMEIWGSQSGVMREVIETAGIRPEEIASIGITNQRETTVVWSRYTGKPIYNAIV
                     WQCRRTSEICDELKNKGLEESIKEKTGLLIDAYFSATKVKWILDNVEGAREKAEQGEL
                     LFGTIDTWLIWNLTRGKVHVTDYSNASRTMMYNINDLEWDEDILRELDIPISMLPLVK
                     PSSYVYGHTDERMLSGAKIPIAGCAGDQQAALFGQNCVEEGTAKNTYGTGCFLLMNTG
                     SNIVKSKHGLLTTIAWGVDGKVTYALEGSIFIGGASVQWLRDELKIIESAKDSEMYAN
                     RVEDTNGVYVVPAFTGLGAPYWDMYARGSILGLTRGAKKEHIVRATLESIAYQTKDVL
                     EAMQNDSKLKLKSLKVDGGASNNNFLMQFQSDILNVDIDRPKIVETTALGAAYLAGLS
                     VGFYIGRNEITSKWSVEKEFNPNMSEEKRCKLYKGWKKAVSRALSWEKEDELDI"
     gene            2646..3188
                     /locus_tag="KPD58_003102"
     CDS             2646..3188
                     /locus_tag="KPD58_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0266833.1"
                     /translation="MPKILENVKEDILKVSRDMILEEDYSNISIRKIAQRCGISAGTV
                     YNYFNSKQEIIEYITKSEWDLLIRRIEYSNKNTEDYIKKLNIIFTEIRNFINKVHNIQ
                     YNDFLNTFETERFFEMKKHKDDFHEQLSNKVYEALEKESFFNNDKLVCNIIIKMFFSY
                     STYLHIEFEDLKPYIEKLIH"
     gene            3202..>5636
                     /locus_tag="KPD58_003103"
     CDS             3202..>5636
                     /locus_tag="KPD58_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0266834.1"
                     /translation="MNHNTSIKNKDSNPKIFLVLTLYLLGIFMGAIDTGIVSPARTII
                     QNSLGVNEKTGIWMITIYTLTYASVIPISGKLADKIGRKYVYLVSIFLFGSGSLICGL
                     SSLFSNFYILLIGRVIQAIGGGGIMPIATAEFGTTFPENKRGMALGLVGATYGIANIL
                     GSSIGSTILSIFGTQNWKWLFFVNLPICLIILIGGVFCIKNNKSESKEKIDKLGTLML
                     ICIIVSLLYGLMNIDFFNFKNSIQDISVYPYLLTFIVLIPIFIFIENRAKDPILSFEY
                     FLNPRILIVLILSLIVGIGMMGMVFVPQYAENALKINAGSGGYFVAILGLFAGIAAPL
                     SGRLIDKFGAKKILLLGFSVSMIGSLYLVLIALKTNTVFSVCVSLIFMGLGMGFTMGT
                     PLNYMMLSNTRLEESNSALATLSLIRSIGTSISPAIMIGFIAHAGLSVQDNIMDLVGK
                     PSTPKIVQLEELENMIDDLKSDPEMAKNLKNVSIPNMNDTSNINMDMTGGKLPKSLLN
                     KVQSADTTNITNITKEISTTMFDEKVPSIITKIQKNVQKGIDGTQSGINGIEDGENKL
                     NSGIKGVQTGIDNMVKARAGINQGIDGVKKGIAGVDKGIKGMEQGLEKQDEAIKELTT
                     AYNKIPTTEKTPTDNENNSNEQNQSNTSNSESDDTKNDSTENKNNNSSENKPNVNEQK
                     ESLNAQIQQLKKTRDDLNTKLQKNKTQKNELSKKLRSMESQKKELQSKLNNSISQKKD
                     MEKSLDTMQQQKKSLQAVLAKTQEVKTEIPKAFDKSKLDYINSIENNRTKIENTLQST
                     LNSGFKQMYITVF"
BASE COUNT         2089 a          867 c          782 g         1898 t
ORIGIN      
        1 gtatactaaa tgcatgttaa aatattattt taaaacatgc acaatatttg tacatattgt
       61 agacatataa acctataaat tatacaatag tattgttaag attatatatt tttttaacat
      121 aaattttgta ttgggggaaa aacaatggat aaaaactata cgatgccaat ataccaaaaa
      181 atagcgttgg atattgcaaa taaaatatac acgggtgaaa tacaagagga ttcagtacta
      241 tttggacgct ctgttttggc tggtaaatac aatgtatctc cagaaacgat aagaagagct
      301 gtcaaaattt tagaggacat tggcgttgtt aaaagcataa agggtaaagg tgttattgta
      361 ctatctccag acaaagcttc ttcttttata aaaaaatata gagatattac aaatatatca
      421 tcatataaat caacacttta taatttaata gatactaaat ctaatttaga aaatgaaata
      481 ttagatacta taaataaaat tttagactat tctaacagac ttgaaataat caatccttta
      541 gtgcccgttc agttcaccat aaattctaat tgcaaatata ttggtcaaac agctgcacaa
      601 actaaatttt ggcaaaatac aggtgctaca atagttgcaa ttaaaagagg tgaagaactt
      661 ataatatctc ctggaccata tattgaattt ttagaaggag acattctctt agtagttggt
      721 gaccaacaca tttacaattc tataccaatg tttttatatg aaaacgaaaa ataaattata
      781 taatttagtg tgattatata aaaatgcaag attaacaaca aaaaaagagc ttcatatttt
      841 acataaagct ctttttaaat tcaaataaat ttaactttga ttaattttta aatatccaat
      901 tcatcttctt tttcccagct taaagctctc gatacagctt ttttccatcc tttatataat
      961 ttacatctct tctcttcact catatttgga ttaaactctt tttctactga ccactttgaa
     1021 gtaatttcat ttcttccaat atagaatcca acagacaacc ctgctaaata agcagctcct
     1081 aaagctgtag tttctactat tttgggtcta tctatatcta catttaatat atctgattga
     1141 aattgcatta aaaaattatt attacttgct ccaccatcaa cttttaatga ttttagtttt
     1201 aattttgaat cgttttgcat tgcttctaaa acatcctttg tctgataagc aattgattcc
     1261 aatgtggctc ttactatatg ttcttttttt gctcctcttg taagtcctaa aattgaaccc
     1321 ctagcataca tatcccaata tggagctcca agacctgtaa atgctggcac tacatataca
     1381 ccattagtat cctctaccct atttgcatac atttcactat cttttgcaga ttcaattatc
     1441 tttaattcat ctctaagcca ttgaacagat gctcctccaa taaatatact tccttctaat
     1501 gcatatgtta cttttccatc aactccccaa gcaattgttg taagtaaccc atgcttagat
     1561 ttaactatgt tactgcctgt attcataagc aagaaacaac ccgttccata agtatttttt
     1621 gcagtacctt cttcaacaca attttgacca aataatgctg cttgttggtc tccagcacat
     1681 cccgcaatag gtattttagc tcctgataac atgcgctcat ctgtatgacc ataaacatag
     1741 ctagaaggtt ttactaatgg caacatgctt attggtatgt ccaattctcg taatatatcc
     1801 tcatcccatt ctaaatcatt tatattgtac atcattgttc tagatgcatt tgagtaatct
     1861 gttacatgta cttttccacg agttaaattc catattaacc atgtatctat agttccaaat
     1921 aataactctc cttgttctgc tttttcccta gctccttcta cattatctaa tatccatttt
     1981 acttttgttg ctgaaaagta tgcatctatt aaaagacctg ttttttcttt tattgattct
     2041 tccaatcctt tatttttaag ttcatcacat atttctgacg ttctcctaca ctgccatact
     2101 attgcgttat aaataggttt accagtatac ctactccaaa caaccgtagt ctctctttga
     2161 ttagttatcc ctattgaagc aatttcttct ggtcttatac ctgctgtttc tataacttct
     2221 ctcattaccc cactttgtga cccccatatt tccatagggt catgctctac ccatccagct
     2281 tttggataaa tttgattgaa ctctttttgt gcaattttca ctatttcgcc ttttttatta
     2341 aatagtatag ctcttgaact ggttgttcct tggtctaatg ccattacata cttcttttcc
     2401 atcaccatat tctccttatc atataatatt taatttatta aaaagaaaaa tttaatatca
     2461 catatttcaa taaataatca tacttaagta caaatttaat ataatagata tttagtaaga
     2521 agacacttat tttctatcat atctatataa taatattata cattatagct tacatattga
     2581 ttaataaatt ttaaaggagt ataataaaca gtgttcataa taattttaat aaaagaggtg
     2641 gtaatatgcc aaagatttta gaaaatgtaa aagaagatat attaaaagtg tctcgggata
     2701 tgattttaga agaagattac tcgaatatta gcataaggaa aatagctcaa aggtgtggta
     2761 taagtgcagg tacagtgtat aattacttta attcaaaaca agaaataata gaatatatca
     2821 caaaatctga atgggattta ttgataaggc gaattgaata ctccaataaa aacactgaag
     2881 attacataaa aaaactaaac attattttta ctgaaataag aaattttata aacaaagttc
     2941 ataatatcca atacaatgac tttttaaaca cttttgaaac agagagattt tttgaaatga
     3001 aaaaacacaa agacgacttt catgaacagc tttcaaataa agtatatgaa gctttagaaa
     3061 aagaaagttt ttttaataat gacaaattag tatgcaatat cataataaaa atgttttttt
     3121 catattcaac atatcttcat atagaatttg aagatttaaa accctatatt gaaaaattaa
     3181 tacattaagg tggtgtttac cttgaatcat aatactagta ttaagaacaa ggacagtaat
     3241 ccaaaaatat ttttagtact tacgctttat ctattaggta tatttatggg agctattgat
     3301 actggaatag tatcaccagc aagaacgatt atacaaaata gccttggagt aaatgaaaaa
     3361 acgggaattt ggatgattac catatacaca ttaacatacg cttctgtaat tcctatctct
     3421 ggaaaattag cagataaaat aggaagaaaa tatgtgtatc tagttagtat atttctattt
     3481 ggtagtggtt cattaatatg tggattatca tcactatttt ccaatttcta tattttatta
     3541 attggtcgtg ttattcaagc aattggtggt ggtggaatca tgccaattgc tacagcagaa
     3601 tttggaacta ccttcccaga aaataaaaga ggcatggctt taggtttagt tggggcaaca
     3661 tatggtattg ccaatatttt aggttcaagc attggtagca caattctaag tatctttgga
     3721 actcaaaatt ggaagtggct attttttgta aatcttccaa tttgtttaat catacttatt
     3781 ggtggagttt tctgtattaa aaacaataag tctgaatcta aagaaaaaat agataagctt
     3841 ggtacattga tgttgatatg tataatcgta tcactattat atggtcttat gaatatagac
     3901 ttttttaatt ttaaaaattc tatacaagat atatcagttt atccatactt attgacattt
     3961 attgtactta tacctatttt catatttatt gaaaatagag ctaaagaccc tattttaagc
     4021 tttgaatatt ttttaaatcc gagaatattg atagtactta ttttatcttt aattgttggt
     4081 attggaatga tgggtatggt attcgttcca cagtatgctg aaaatgcatt aaagataaat
     4141 gctggttctg ggggatattt tgtagctata ttgggattat ttgcaggtat tgctgctcct
     4201 ttaagcggaa gacttattga caagtttggg gcaaaaaaaa tactattact aggattttct
     4261 gtatctatga taggctctct ttacctagtt ttaattgctc taaaaaccaa tactgtattt
     4321 agtgtatgtg taagtttaat atttatggga ttaggaatgg gatttactat gggaactcct
     4381 ctaaactata tgatgttatc aaatacaaga ttagaagaat ctaattcagc acttgcaaca
     4441 ttatccttga tacgctcaat tggtacttca atttctccag ctataatgat tggatttata
     4501 gcacatgcag gattatctgt gcaagataat attatggact tagttggaaa gccctcaact
     4561 cctaaaatag ttcaacttga agaacttgaa aacatgatag atgacttaaa atcagaccct
     4621 gaaatggcaa aaaatttaaa aaatgtctct atcccaaata tgaatgatac ttcaaatata
     4681 aatatggaca tgacaggtgg caaacttcca aaatccttac ttaataaagt tcagtcagca
     4741 gatacaacca acattaccaa tataacaaaa gaaatatcaa caacaatgtt tgatgaaaaa
     4801 gttccatcta taattactaa aatacagaaa aatgtacaaa aaggaataga tggaacacaa
     4861 agtggaataa acggtattga agatggtgaa aataaactaa atagtggtat taaaggagtt
     4921 caaactggta ttgacaatat ggtaaaagct agagctggta ttaatcaagg aatagatggt
     4981 gtaaaaaaag gtattgctgg tgttgataaa ggtataaaag gtatggaaca aggtcttgaa
     5041 aaacaagatg aggccatcaa ggaacttaca actgcttata ataaaatacc tactacagaa
     5101 aaaactccta ctgataacga aaataactca aatgaacaaa accagagtaa taccagtaat
     5161 tctgaatcag acgacactaa gaatgattct acagaaaata agaataacaa ttccagcgag
     5221 aataaaccaa atgtaaatga acaaaaagaa agtttaaatg ctcaaataca gcaactaaaa
     5281 aagacaagag atgacttaaa tacaaagtta caaaaaaata aaactcaaaa gaacgaatta
     5341 agtaaaaaac taagatcaat ggaatctcaa aagaaagagc tacaaagcaa gttaaataat
     5401 tctataagcc aaaagaaaga catggaaaaa agccttgata caatgcaaca gcaaaaaaaa
     5461 tcactccaag cagttttagc aaagacacaa gaggtaaaaa ctgaaatacc aaaggctttt
     5521 gataaatcaa aattggatta tataaattcc atagaaaata atagaacaaa aatagaaaac
     5581 acactacaat ctactctaaa ctcaggattt aaacagatgt atataacagt attctg
//
LOCUS       DAELLV010000219         5593 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.219, whole genome shotgun sequence.
ACCESSION   DAELLV010000219 DAELLV010000000
VERSION     DAELLV010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5593
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.219"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..659
                     /locus_tag="KPD58_003104"
     CDS             <1..659
                     /locus_tag="KPD58_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266835.1"
                     /translation="NIVQYNFADIEEEVYSLDYAIAWNTDEENVNIIPFTNKFCKESI
                     ESFCLGKINNFVEILNEGFVENHHYVHLDKMISVPKKKVNLVYQQDTHGYLLRDDNDN
                     LIPAKITSEQSKSISSKMELFSAGEEKCLINILLKADPSYILDVDSIKDKNILNLGYE
                     SIDRYKEYNFDDDKILIFFINKKRYSVIMKKTNNSDNDLVSRNNAIKELFTNKAGNLN
                     "
     gene            complement(1165..3867)
                     /locus_tag="KPD58_003105"
     CDS             complement(1165..3867)
                     /locus_tag="KPD58_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG0266836.1"
                     /translation="MKNVSKKFFVCTIIVLITFLCFSKFAIYVFDKQEKKETKVVENM
                     LNVYKNNIILNLTEKFNYSKDLTSVLVSNNYSIDKLKPIADEILKNNSCIYISVVEKY
                     NVAAFFSNSVKKDIINTPIQELNYSYSLANSIKGLVVEGPSKDQFSNGDVFLITNPIY
                     KNGEYWGEITVALDAKKVVDSFNLNKLKESGFEYELWRVHLDNGSKSTIEISTKDFNF
                     SKSHEIKFKLPTVWTLSIVYKNGWNNKNIDFIVFVILLIISILIGVMTFIYIEKKSIS
                     KELNQQSLKNLETGILNREGFLIKLQEHLNRKNNEIVLLYICITNYSKFAPVMSFEKR
                     QDYLTHIVKNLDNYIKSSHFIARISEEGFVLALEENCDKNELLNIEKGISLELLRKIK
                     VENKQVFLTIGYGSSRCVDDRKDAESLLLSAIEDYERRFPAMSPIHDFTKKCEDMLDE
                     KKQIVFGEYPDLEINKLSKVIYKYYKKVQRIMYKDYNIDIGNRRGYMRDVEVLIDYNS
                     ERMLYLFLIDICKFGKFNELFSIEIGDVILKEVSNKLKTIFSNYLYRINGDVFLGISI
                     DKESYLDTLHKVNEVFNSSLSVEDVLFDIKCNIGICQYPYHGENPYDLLEKVQIALEY
                     AKKDVKSNVKVYDKELTEEIYHENNIIKDLKKGLEEQTLEVWYQPIFNVSKNCYIKAE
                     ALIRLRNKEGGYYSAYEVITYAEKHGLIKDVGLYVLKKACDVTTKIKDKNIDFEQMQL
                     NLSVQQIVHKQCANDILSCIENCGLDKKYICVEVTETVLMETFEIVNVTLDKLKKEGI
                     SIALDEFGSGFSSINYLANLPIENLKIDKELVEQITYSKKQFELLKTIVQIAKINGMD
                     IVAEGVETKETLDLVVTSGVNLIQGHYFSEPLSEDKFIELMIEE"
     gene            complement(3893..5443)
                     /locus_tag="KPD58_003106"
     CDS             complement(3893..5443)
                     /locus_tag="KPD58_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG0266837.1"
                     /translation="MRYKKRNIDKSSELSFQSLAKYFITFYIVIILIILSMFCINTIW
                     VMKKDNQDLLSTTSNRIEDRVGETIKLLNSLSKDNYIIDNSVNGIDKSKELSMYSDEF
                     GYMMICFVDKNIIVWDEEGGNISLAGRDYMQKLFATKKYQVTDSFAAGADGKTLNYTI
                     ASPIIKNGNVDGCVFAAIYFDEIKDILKQGKGQQQQNFILFGSENQIMNLTSDETAYG
                     EKYMKLSQKTKYLGTNAKKVEKDMFNNKSGDYWAFENGKLYFMSYKPIKNTNWSLFCK
                     VNFLDIFALNLIPMGAILSVITLVFIILICMEKSFMKKRMEVIDMLLGSVQELEKRIY
                     QTERPSEVDFKEIIQLTSKGLTDGLTGVVTRTVFFDQIYNRVEMEENDKLSVISFVDL
                     DDLKCINDLYGHEIGDMVLKNVGYVLREYEIKYNGLIGRYGGDEFVMLLSGFTSREEV
                     EDLLDEIAIRLHTEVLTDDGIIQTHCSVGAVVCSVKDNKDIDNLLKQADEALYYVKRN
                     GKGNYHLV"
BASE COUNT         2005 a          920 c          538 g         2130 t
ORIGIN      
        1 gaaacatagt acaatataat tttgcagata tagaggaaga agtatattcg ttggattatg
       61 ctatagcatg gaatacagac gaggaaaacg taaacataat accatttaca aataaattct
      121 gtaaagaatc tatagaatct ttttgcctag gtaaaattaa taactttgtt gaaatattaa
      181 atgaaggttt tgttgaaaat catcattatg tacatttaga taaaatgatt agtgttccta
      241 agaaaaaggt taatttagta tatcaacaag acacacatgg ttatcttcta agagatgata
      301 atgacaacct tattcctgca aaaataactt cagagcaatc taaatctata tcaagtaaga
      361 tggagttatt ctctgctggt gaagaaaaat gtttaatcaa tatattacta aaggctgacc
      421 catcatatat tttagatgta gatagtataa aagacaaaaa cattctaaat ctaggttatg
      481 agtctattga cagatataaa gaatacaatt ttgatgatga taagatttta atatttttta
      541 taaataaaaa acgttattca gtgattatga aaaaaacaaa caactctgat aacgatttag
      601 tttccagaaa caatgctatt aaagaattat ttactaataa agcaggaaat cttaactaat
      661 aatataattt taaattatgt tattgattaa attaataata gtatacaaag aggctgtctc
      721 aaaatagaga taagcttctt tttcattgtt gaaataataa aatatattta attctatata
      781 aaagagagtg cctcataaac taaaaagttc attttgagac accctcctat ttataataaa
      841 caaataaatc cttaaaccta tcgagtgatt ataattttta gattgggtta tcttgcattt
      901 ttaaatttaa ggtaactttc tactctaaac tttaatctct aatcaatatc atcaactgta
      961 acaacttaca attaaatctt taataatcaa actttagact atctatatta aaactcaaat
     1021 tagatttagg atttatttgg atatcacatt tattttatta actactaatc aattattttt
     1081 acttataaac ttattcttcc ctaattctat tattaaatac ttttaattat ttttcaatac
     1141 cttaaatctt tattttatta acctttattc ttcaatcata agttcaataa atttatcttc
     1201 acttaaaggt tctgaaaaat aatgaccttg aatcaaattc acacctgaag ttacaactaa
     1261 atccagagtt tcttttgttt caacaccttc tgctacaata tccatgccat taatcttagc
     1321 aatttgaact atagttttta ataattcaaa ttgtttttta gaataggtaa tttgttctac
     1381 taattcctta tctattttta aattttcaat aggtaaattt gccagataat ttatactact
     1441 aaatccagaa ccaaactcat ctaaagctat gctaatgcct tcttttttta gtttatcaag
     1501 agtaacattt acaatttcaa aagtttccat aagtactgtt tctgtaactt caacacaaat
     1561 atacttttta tctagaccac aattctcaat acatgataat atgtcatttg cacactgctt
     1621 atgtacaatc tgctgaacag ataaatttaa ttgcatttgt tcaaaatcta tatttttatc
     1681 ttttattttt gtagttacat cacaagcctt ttttagtaca tatagaccta catccttaat
     1741 aagtccatgc ttttctgcat aagtaataac ttcataggca gaataataac cgccctcttt
     1801 atttctcaat ctaattagtg cctcagcttt tatataacag tttttactaa cattaaaaat
     1861 tggctgatac caaacttcta gtgtttgttc ttccagacct ttttttaaat ccttaataat
     1921 attattttca tggtaaatct cttctgtcaa ttccttatca taaactttaa cattactttt
     1981 gacatccttt tttgcatact ccaatgcaat ttgtaccttt tccagaaggt catatggatt
     2041 ttcaccatga tatggatatt gacaaatacc aatattacat tttatatcaa aaagtacatc
     2101 ttctacactt aaagaactat taaatacttc attaacctta tgtaaagtat ctaagtaaga
     2161 ttctttatct attgaaatgc ctaaaaatac atctccatta atacgatata gataattaga
     2221 aaaaattgtt tttaatttgt tactaacttc ttttaaaata acatctccta tttcaataga
     2281 aaacagttca ttaaattttc caaacttgca tatgtctatt aaaaataagt ataacattct
     2341 ctcagaatta taatcaatta atacttcgac atcacgcata taccctcgtc tatttccaat
     2401 atcaatatta taatctttat acataatacg ctgtactttt ttataatatt tgtatattac
     2461 tttagacaac ttgtttattt ctaaatctgg atattcacca aatacaatct gtttcttttc
     2521 atcaagcata tcttcacact ttttagtaaa atcatgaata ggagacattg ctggaaatct
     2581 tctctcataa tcttcaatag ctgaaagtaa cagagattct gcatcttttc tatcatcaac
     2641 acacctagat gagccataac caatcgttaa aaatacttgt ttattttcta ctttaatttt
     2701 tcgtaaaagc tctaaggaaa tccctttttc aatgttaagc aattcatttt tatcacaatt
     2761 ttcttctaaa gctagaacaa aaccttcctc acttatacga gcaataaaat gagaactttt
     2821 tatgtaatta tctagatttt ttactatatg tgtaagataa tcttgtcttt tttcaaagct
     2881 cataactgga gcaaatttac tataattagt aatacatata tacagcaaca ctatttcatt
     2941 attttttcta ttcagatgct cttgtaactt aattaaaaaa ccttctctat tcaaaatacc
     3001 agtttctaaa tttttaagac tctgttgatt taattcctta ctaatcgact tcttttcaat
     3061 gtaaataaat gtcatcacac ctattaatat ggaaataatt aacaatataa caaatacaat
     3121 aaaatctata ttcttattat tccatccatt tttgtacaca atgctaagag tccaaacagt
     3181 cggaagttta aacttaattt catgtgattt ggaaaaatta aaatctttag tagaaatttc
     3241 tattgtagat tttgaaccat tatctagatg tacacgccaa agttcatact caaatccaga
     3301 ttcttttaat ttatttaagt taaaagaatc tacaactttt ttggcatcta aagctactgt
     3361 aatttctccc cagtactctc catttttata aataggatta gtaattaaaa acacatctcc
     3421 atttgaaaac tggtctttag aaggaccttc tacgactaat cctttgatag aatttgctaa
     3481 tgaataagaa taattcagtt cctgtattgg agtattgata atatcttttt tgacagaatt
     3541 agaaaaaaat gcagctacat tatatttttc tacaactgaa atatatatgc aagaattgtt
     3601 tttaagaatt tcatctgcaa taggttttaa tttatctata gaataattgt ttgaaactaa
     3661 aactgatgtc aagtctttgc tataattaaa tttttcagtc agatttaaaa taatgttatt
     3721 cttgtagaca tttaacatgt tttctactac ttttgtttct ttcttttctt gtttgtcaaa
     3781 aacataaata gcaaatttac taaaacataa aaatgttatt aatacaataa tagtacatac
     3841 aaaaaatttt ttagatacat ttttcatatg atataaactc cttccacata cattaaacta
     3901 aatgataatt accttttcca ttacgtttaa catagtataa agcttcatca gcctgtttta
     3961 ataaattatc tatatcttta ttgtctttta cactacatac tacagctcca acactacaat
     4021 gagtttgaat gataccatca tctgttagta cttcagtatg taagcgaata gcaatctcat
     4081 ccaataaatc ctccacctct tctctcgatg taaaaccaga taacaacata acaaattcat
     4141 ctccaccata gcgcccaatt aaaccattat attttatctc atattcacga agtacatatc
     4201 caacattttt caaaaccata tccccaattt catgtccata taagtcgtta atacatttca
     4261 aatcatctaa atcaacaaat gaaattacag ataatttatc attttcctcc atttcaacac
     4321 gattataaat ttggtcaaaa aatacagtac gtgttacaac accagttaat ccatcagtca
     4381 gacctttact tgtgagctga ataatttctt taaagtctac ttcactaggt ctttctgttt
     4441 gatatatacg tttttctaat tcttgaacag aacctaaaag catatcaata acttccattc
     4501 gttttttcat gaagcttttc tccatacaaa tcaaaataat aaatactaaa gttataacag
     4561 atagaatagc tcccatagga attaaattta atgcaaaaat atctaagaaa ttaactttac
     4621 aaaataaact ccaatttgtg ttcttaattg gtttatagga cataaagtat aattttccat
     4681 tttcaaaagc ccaataatca cctgatttat tattaaacat atccttttct actttttttg
     4741 cattggttcc aagatatttt gttttctgcg ataacttcat atacttctct ccatatgcag
     4801 tttcatcact tgttaaattc attatttgat tttcacttcc aaataatatg aaattctgtt
     4861 gctgttgacc ttttccttgt ttgagtatat cctttatctc atcaaagtaa attgctgcaa
     4921 atacacaacc atctacattt ccattcttta ttataggtga tgcaatagtg tagtttaaag
     4981 tttttccatc agcccctgct gcaaaactat ctgtaacttg atattttttt gttgcaaaaa
     5041 gtttctgcat ataatcacga ccagctagac taatattacc accttcttca tcccatacaa
     5101 ttatattctt atcaacaaaa catatcatca tatacccaaa ttcatcacta tacattgaaa
     5161 gttctttact tttatctatg ccattcacac tattatctat tatgtaatta tctttactca
     5221 aagagttcaa tagcttaatt gtttctccta ccctatcttc aatacgattt gacgttgtag
     5281 ataataaatc ttgattatct tttttcataa cccatattgt atttatacaa aacatagaaa
     5341 gaataattaa tataattact atatagaaag ttataaaata ttttgctaga gattgaaatg
     5401 aaagttcact tgacttatct atatttcttt ttttgtacct cataattcct acccccacta
     5461 tagaaaaatt gatgaaaatg ttcctattag aaccttaata atacattaca aaacacataa
     5521 tttcaagtaa aacgttaaat tttcattact aaatataaac tttttattac aatattattt
     5581 tttatatcct att
//
LOCUS       DAELLV010000220         5591 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.220, whole genome shotgun sequence.
ACCESSION   DAELLV010000220 DAELLV010000000
VERSION     DAELLV010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5591
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.220"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            7..1287
                     /locus_tag="KPD58_003107"
     CDS             7..1287
                     /locus_tag="KPD58_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266838.1"
                     /translation="MYSVFKIIENNRVYSFVSSKLDEINRDYKTSEFYKKVRDDYYTY
                     SKENPYADVNITSFIEEVVSDLKHNNLPLLEKIRSIKNSSSISILLGVLGTFVGLSTM
                     LLCVDTKDIINSLPSTISSMQTAFTTSIFGVVFSLIIGYFTKIKDCEHVLIQIMLKSE
                     NLLTSEITHFKSERMDLKVEEVKNTIKQISKSIEAIEKFDKISKDLNDFNDEFISGIE
                     ALKSLLEGSQSSIKTFDQSVRKLDKQFNILNIKFVKLFDKYDNQDNINKEILFDIKES
                     SKNIYNATESQFKIRDYIKNINAGFALYERSAQDLLTKLMTHENKISQNQKILLDEKF
                     TLDDSIKNLSSIIENFSNDLQVKLDMMFENSLDIQDKLDVMFNNSFMNDEVPLDSEEL
                     FKDDVNNVFNPFSEEIYEIEDKEIKVIGEDELNE"
     gene            1280..1912
                     /locus_tag="KPD58_003108"
     CDS             1280..1912
                     /locus_tag="KPD58_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBG0266839.1"
                     /translation="MNNKYRRTVNREFEKSSFWPTFTDLLSTVLMVVILILFSSESIS
                     GSVEQDLAKNVNASVEETFKKSGIPVKVDKSNGQVTFGEKTMFDVDSDVLKPEAKEML
                     KMFVPKYIETIYKDYGDYISKIVIEGHTDDVGSYIYNLDLSQRRAYSVAKFIVGDEIG
                     DYKYKDKVTKHIIAIGRSKAELIKNGDNSVNRDASRRVELKYEININQNK"
     gene            2241..3398
                     /locus_tag="KPD58_003109"
     CDS             2241..3398
                     /locus_tag="KPD58_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0266840.1"
                     /translation="MIEISIKNKKNSKIKTLILLLIVSISIYLTGCTTSDNGNNQSNN
                     NDSLSSSISSDKNVKIHFIDTGNSDAILIQDGKTFTLIDGGDNDDEGLMVDYLNNQGV
                     KDIKYLIATHSHADHLGGLDSVVKNFNIENVFVSNGSAETKSYRDFINALANKGLSPS
                     VPLENNKFYLEDSYFEVLNTNGGDTTNEQSLVLVYTNGNDKVLFTGDAEEGTEKEILP
                     KLEKVDLLKVAHHGSRSSSSQEFLDKVNPEYAVLLVGKGNSYGHPHQETMNKLKKMGV
                     KVHRSDECSDIIFESTGDGVFTSCKDGSYNKGVREDGNSGKSNSNTSKNDSFKNKQNT
                     SNSEEIVYFTPKGKSYHSTKNCSGLSRSKKILSGTIAESKKNDPCDICYGK"
     gene            complement(3545..4180)
                     /gene="lexA"
                     /locus_tag="KPD58_003110"
     CDS             complement(3545..4180)
                     /gene="lexA"
                     /locus_tag="KPD58_003110"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HBG0266841.1"
                     /translation="MYLDLTEKQVLILEFIKSQIILKGYPPAVREICTAVGLRSTSTV
                     HSHLNKLEKLGYIRKDPTKPRAIEVLERSKVNDVSGANQEIIELPLVGQITAGEPILA
                     QQNIEEYIPFPASLVKGSNNFVLRVKGESMINAGILDEDYVVVDKKNTALNSQIVVAL
                     INGESATVKRFFKEGNLIRLQPENDFMEPIMLNDSEVEIVGIVTGVFRVIK"
     gene            complement(4284..5411)
                     /locus_tag="KPD58_003111"
     CDS             complement(4284..5411)
                     /locus_tag="KPD58_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG0266842.1"
                     /translation="MNNDKKIIKVHNKSDKPDNIVIKENELIEKCLLLENKLPVFMKD
                     YFIYLKGSVAVSTRLAYLEDINFFCSYLIETKELTNADCVKDITEDDFNTIKSRDINL
                     FLGDYCSRYYKNTEKNTLIFENNNRALARKKSSISTLFKFLYRNSQIDNNITDGFNPI
                     KLPKPQPDAIKRLEIDEVAKMLESVETGEGLTEKEKVYWRKTKLRDKAILALFVTYGL
                     RLNELRELNISSFNFSRGEFKIYRKRGKEVLMPINHTCEHVIKDYLQNERTRDELLND
                     EEKDALFLSLQNKRITAKAIRTLVKKYTSIPLDTTRDNGYSPHKLRATAATSLIQTGF
                     SIYDVQNLLDHDNVTTTQLYAAHKKNVKRDIVKNFEWIEDD"
BASE COUNT         2084 a          695 c          824 g         1988 t
ORIGIN      
        1 ataactgtgt attctgtttt taaaataata gaaaataaca gagtatattc atttgtaagt
       61 tcaaaattag acgaaatcaa tagagactat aagacaagtg agttttataa aaaggtaagg
      121 gatgactact atacatatag taaagaaaat ccttatgcag acgtaaatat aacttcattt
      181 atagaggaag ttgtatctga tttaaagcat aataatttgc cattgttaga aaaaattaga
      241 agtataaaaa attcatcttc aataagtata ttacttgggg ttttaggtac gtttgttggt
      301 ctttctacaa tgcttttatg tgttgatact aaagacatta taaattcact tccttcaact
      361 attagtagta tgcaaactgc atttacaact agtatatttg gagttgtatt ttctctaatt
      421 ataggttact ttactaagat aaaagattgt gaacatgttt tgatacaaat aatgttaaaa
      481 tctgaaaatt tactaacatc tgaaataact cattttaagt cagaaagaat ggatttaaaa
      541 gtagaagagg taaaaaatac aataaaacaa ataagtaagt ctatagaagc tattgaaaaa
      601 tttgataaaa tatctaaaga tttaaatgac tttaatgatg aatttatcag tggtatagaa
      661 gctttaaaaa gccttttaga aggttctcaa agttctatta agacatttga ccagagcgta
      721 agaaaattag ataaacagtt taatatttta aatataaaat ttgtaaagct ttttgataaa
      781 tatgataatc aagataatat aaataaagag attttatttg atataaaaga atcttcaaaa
      841 aatatataca atgccactga aagccaattc aaaattagag attatataaa aaatataaat
      901 gcaggatttg ctctatatga aagaagtgct caagatttat taactaaatt aatgacccat
      961 gaaaataaaa tatctcaaaa tcaaaaaatc cttttagatg aaaagtttac cttagatgac
     1021 tcaataaaaa atctctcaag tattattgaa aacttttcta atgacctaca agttaaactg
     1081 gatatgatgt ttgaaaattc tttagatatt caagataagt tagatgttat gtttaataac
     1141 tcttttatga atgatgaagt tcctcttgat agtgaagaat tatttaagga tgatgttaat
     1201 aatgtattta atccttttag tgaagaaatt tatgagatag aggataaaga aataaaggtc
     1261 ataggtgagg atgagttaaa tgaataataa atatagaaga actgttaaca gagaatttga
     1321 aaagagtagc ttttggccaa catttacaga tttattatct actgtattga tggttgtgat
     1381 tcttatctta tttagctcag aaagtatttc aggttctgtt gaacaagatt tagctaagaa
     1441 tgtcaatgct tctgttgaag aaacttttaa aaaaagtgga atacctgtga aggtagataa
     1501 atctaatggg caagtaactt ttggtgaaaa aaccatgttt gatgtagata gtgatgtttt
     1561 aaaaccagag gctaaagaaa tgctaaaaat gtttgttcca aagtacatag agactattta
     1621 taaagattat ggtgactata tatccaaaat agttatagaa ggtcatacag atgatgtggg
     1681 aagttatata tacaacttag atttatcgca aagaagagca tatagcgttg ctaaatttat
     1741 agtgggagat gaaataggtg actataaata caaagataaa gtgactaagc atattattgc
     1801 aataggaagg tcaaaagctg aactcatcaa aaatggtgac aatagcgtga atagagatgc
     1861 atctagaaga gtagaactaa aatacgaaat aaatattaat caaaataaat aattaaattt
     1921 ttataaaaaa aaagaaggca gttggtgtga gctttaagcc ttctttaagg aaaaagttta
     1981 tttatttttg ttgatactta acatattata gtttttagaa gcagtttgca atatattaaa
     2041 cgtaaatggt cgtttaaaat gcataactgg atactactga tgtaaaatat ttcattttat
     2101 ataatataaa aattatacag tgtcaatttt ctatattatt gtactataat ctattattta
     2161 aaatattatg taaaaaaata tcataaaaac gcatatcatg ttataattac tagagtatta
     2221 tgaatatgga ggcgtagttt gtgatagaaa taagtataaa aaataaaaaa aattctaaaa
     2281 ttaaaacttt gatactttta ttgatagtat ctatatcgat ttatttaact ggttgtacta
     2341 catcagataa tggaaataat caatcaaata ataatgattc tttgtctagt agtatatcaa
     2401 gtgataagaa tgtaaaaatt cattttattg acactggaaa tagtgatgca atattgattc
     2461 aagatggtaa aacttttaca ttgatagatg ggggagataa tgatgatgag ggattaatgg
     2521 tagattacct aaacaatcaa ggtgtaaaag atattaaata tcttatagct acccattctc
     2581 atgcagacca tttaggtgga cttgattctg ttgtcaaaaa ctttaatata gaaaatgttt
     2641 ttgtatcgaa tggttcagca gaaacaaaga gttataggga ctttataaat gctttagcaa
     2701 ataagggttt atccccaagt gtaccgcttg aaaataataa gttctattta gaagattctt
     2761 attttgaagt tctaaataca aatgggggag atactactaa tgaacaatct ctagtccttg
     2821 tatatactaa tgggaatgat aaagttttat ttacaggtga tgctgaagaa ggtaccgaaa
     2881 aagaaatact acctaagctt gagaaagtag accttttaaa agtagctcat catggttctc
     2941 gtagtagttc atctcaagag tttttagata aagtaaatcc tgaatatgcg gttcttttag
     3001 taggcaaggg taattcatat ggtcatccac atcaggagac aatgaataaa ttaaaaaaaa
     3061 tgggagtaaa agtgcataga agtgatgagt gttcagatat aatatttgaa tcaacaggtg
     3121 atggagtatt tacaagttgt aaagatggaa gttataataa aggcgttaga gaagatggaa
     3181 actctggaaa atcaaacagt aatacaagta aaaatgattc ttttaaaaat aaacaaaata
     3241 catccaattc agaagaaata gtgtatttta ctccaaaagg taagagctat cactctacaa
     3301 aaaattgtag tgggctttca agaagtaaaa aaatattatc aggcacaatt gcagaaagta
     3361 agaaaaatga cccatgtgat atatgttatg gaaaataaca aaaaaacaac caatttatct
     3421 ataaagcttc tgaactttaa atcattcaaa aaataaaata aatgcataaa aaaataagct
     3481 ataatcaata gataacacat tttatctata cgaatagctt atttttttaa gtatttttta
     3541 atagctattt aataactcta aatacaccag ttacaatacc tacaatttca acttctgagt
     3601 cattgagcat aattggttcc ataaaatcat tttctggctg aagtcttatt aaatttcctt
     3661 ctttaaaaaa cctcttaact gtagcagatt caccatttat aagtgcaact actatctgag
     3721 aatttaaggc tgtatttttt ttgtctacta caacgtaatc ttcatctaaa attcctgcat
     3781 taatcatact ttcgcctttt actcttaata caaaattgtt actgcccttt actaaactgg
     3841 cagggaatgg aatgtattcc tctatgtttt gttgagctaa aatgggttct ccagctgtta
     3901 tttgacctac taaaggaagc tctattattt cttgattagc tccagaaaca tcatttactt
     3961 tactacgttc taaaacttca atagctcttg gtttagtagg gtcctttctt atgtatccca
     4021 gtttttcaag tttatttaga tgagagtgca cagttgaagt agatcttaac cctacagcag
     4081 tacatatttc tcttacagca ggtggataac cttttaatat gatttgagac tttataaatt
     4141 ccaatatcaa gacttgcttt tcagttagat ctaaatacat aacatcactc atttccaaac
     4201 atattttgtt tttatttcca tacactttat atttttaatt ataacataat ccatttatat
     4261 ctgcaaacat ttgttcctga tttttaatcg tcctcaatcc attcaaaatt cttgactata
     4321 tcacgtttta catttttctt atgagctgca taaagctgag tagttgtgac attatcatgg
     4381 tcaagtaagt tttgaacatc atatatagaa aatcctgttt ggattagaga agttgcagcc
     4441 gttgctctta atttatgggg actatatcca ttgtctctag tagtatcaag tggaatagat
     4501 gtatattttt ttaccagtgt tcttatagcc tttgctgtaa ttctcttatt ttgaagagac
     4561 aaaaataaag catctttttc ttcatcattt aatagttcat ctcttgttct ttcgttttgc
     4621 aaataatctt ttattacatg ttcacaagta tgatttatag gcattaaaac ttctttccca
     4681 cgttttctat atattttaaa ttcacctctt gaaaaattaa aagaggagat attgagttct
     4741 ctaagctcat ttaatctaag cccgtatgta acaaataatg ctagtatagc tttgtcacgt
     4801 aatttagttt ttctccaata taccttttct ttctctgtaa gcccttcacc tgtttctact
     4861 gattcaagca tcttagcaac ttcatctatc tcaaggcgct taatagcatc tggctgaggt
     4921 ttaggaagtt ttattggatt aaatccatca gttatattat tatctatctg agagtttctg
     4981 tataagaatt taaatagagt agagatggat gatttttttc ttgctaaagc tctattgtta
     5041 ttttcaaaaa tcaatgtgtt tttttctgta tttttgtaat atctactgca ataatcacct
     5101 aagaataaat ttatatctct tgattttatt gtattaaaat catcttctgt aatatctttt
     5161 acacaatctg catttgttaa ttctttggtt tcaatcaaat atgaacaaaa aaaattaata
     5221 tcttctaaat aagcaagtcg tgtagaaact gcaacagaac cttttaaata tataaaataa
     5281 tccttcataa atacaggaag tttattttcc aaaagtagac atttttctat gagttcattt
     5341 tctttgataa caatattatc aggtttatct gatttattat gtacttttat aattttctta
     5401 tcattattca aaattaaacc tcctaaaact aaaatataaa tcttttatat tattttttga
     5461 tatttatacc aagtgagtat aaatatttaa tttaatcatt ttttacattt ttaagagatt
     5521 agcagtactt tagatgaaat tatcactaac tattccctaa atccacattt aaagactagt
     5581 tgaatttaat t
//
LOCUS       DAELLV010000221         5535 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.221, whole genome shotgun sequence.
ACCESSION   DAELLV010000221 DAELLV010000000
VERSION     DAELLV010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5535
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.221"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     regulatory      63..165
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            259..1635
                     /locus_tag="KPD58_003112"
     CDS             259..1635
                     /locus_tag="KPD58_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBG0266843.1"
                     /translation="MQTTNSSTMQKLFPILTNKNVDMKKEIIAGVTTFLTMAYIIAVN
                     PNILSETGMPAGALVTGTCLAAAFGCILMGVVANLPFALASGMGLNAFFAYTVVLQMG
                     VPWEVALTAVFVEGIIFIVLSVSGVREAVVNAIPKNMKLAVTGGIGIFIALIGLVNSG
                     IVIGDQATLIKMGRFTPAVIITCVGLIIIAVLDKKRVKGSILFGIVVSSLLAWAFAFI
                     NPEYAQKLGIYLPGGLFKFESLAPIAGKIDLGYVLHPTNIGGFLVVVCTFLFVDFFDT
                     VGTLVGVCSKANMLDEKGNVPNVGRALLTDAVATTVGAGLGVSTVTTYVESSTGVIAG
                     GRTGWTAITVGILFLAAMFFSPVFIAIPSCATAPALIYVGYLMLGTVKDIEFDNITEG
                     VPAFVTIACMALTYSIGDGLTLGILTYVFVNIFYNIFGAKKAEDKKHVSVVMIVLAIL
                     FIIKLWLL"
     gene            complement(1749..2894)
                     /locus_tag="KPD58_003113"
     CDS             complement(1749..2894)
                     /locus_tag="KPD58_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG0266844.1"
                     /translation="MNTFEQLKISSTLIDGLKKQDITSPTEVQSLVIGNIIQNKDLLI
                     NSQTGTGKTLAYLLPIFEKIDTSKRETQALILAPTHELVMQITNQVELLAKNAELSVT
                     SLALIGEVNIQKQIKNIKAVKPHIVIGSCGRVLDLIKQKKLKSHNIKTIVLDEVDNLL
                     NGKNITCIEDIIRTTLRDRQIIGCSASLTDSTIKICDKLMKEFEIIKTKEKSQINPNI
                     NHSYLLGEIRDKFTFLRKALAATNPKKAIVFVNNEKNIEVIVSKLNYHNYKAIGIFGN
                     MEKEDRKNAINKFKLGKAKILITTDLSARGLDIVDVSHVFNLDFPKSKNEYLHRCGRT
                     ARGNRSGNTISIITKKELDIIKDLQKEFNIVITPKTLQNGELIDIIK"
     gene            3147..3854
                     /locus_tag="KPD58_003114"
     CDS             3147..3854
                     /locus_tag="KPD58_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0266845.1"
                     /translation="MLKVINVSKSYKNKKVVSSVSFDVNDGELFGFIGHNGAGKTTTI
                     KAIVGIHDFEEGEVLINSKSIKKQPVECKKEMAYIPDNPDLYESLTGIQYLNFIADIF
                     EVAKDKREELIKYYSNKFELNKALGDLISSYSHGMKQKLAIISALIHSPKILILDEPF
                     VGLDPKASFILKEIMREFCDKGGCIFFSTHVLEVAEKICDKIAIIKDGNIIAYGTTEE
                     VKGSNSLESIFMELIEK"
     gene            3901..5496
                     /locus_tag="KPD58_003115"
     CDS             3901..5496
                     /locus_tag="KPD58_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0266846.1"
                     /translation="MSNLSILIKNNIINEFKLNALKKADGREKQKIMGMMLSVVIIAG
                     LLIFYVTSLSLSLVDILKQINQLEMLLIFGFGLSTLTTIVTSLYKTSSYLFQAKDLDL
                     LTSLPIKESTVLASKILMLVGANYLFSSICMIIPAVVYFLNSDVSITYFPYLILLFLA
                     LPLLPIVASSIIFLFIGQISSRIKYKNLVLIIGSIILMLLYTLLMSRLNSMSVEMLKN
                     SVSISETIRKVYPPIYYFVDALKTGSIVSFLAFIAISIIPFFMFVTLFAKKFKDINAK
                     MSEGYKSKNYEFKGQKTSRPSKALLKKEFKRYTSSFIYFLNSSAGLLFLIVGTILILL
                     FGADKVGELLKLNIDFNLIKIQLLGVFFFIIVMNCTTYCSISLEGKNLWILKSSPIDE
                     REIFWSKILMNFLLNAPLSILCLILVSIRLDFEIQFIIVSICLIGSFSMFVALMGLLS
                     NLLYPNLDWKNEVAVVKRSASMIVTMLVSLAYVIILGGIYVLLGISFLNIYIILASIL
                     TLILDFMIWRIISTKGVKIFKNL"
BASE COUNT         1869 a          651 c          865 g         2150 t
ORIGIN      
        1 aatatatacg tatatattga caaaattaat tatataaatt aaaataatat tcgtaaagaa
       61 aaattcatgt tttgtactgt ataacatata attttgacaa tatgggtcat aagtttctac
      121 cggaataccg taaatattct gactatgtat actattattt agttaattaa atatttaatt
      181 aactgaatta tgaaggatac ggctcagctt atcaactact gtgtcgtttt tttgtaaaat
      241 ttaataggag gtgttgttat gcaaacaaca aacagtagta ctatgcagaa actttttcca
      301 attttgacta ataaaaatgt cgacatgaag aaggaaataa ttgcaggagt aactactttt
      361 ttaactatgg cttatataat agctgtaaat ccaaatattt tatctgagac aggcatgcct
      421 gcaggtgcac ttgttacagg tacttgttta gcagcagcat ttggatgtat tttaatgggt
      481 gtagttgcta accttccttt tgctttagca tcaggtatgg gtcttaatgc attttttgca
      541 tatacagtag ttttacaaat gggtgttcct tgggaagtag ctcttacagc agtatttgta
      601 gaaggtatta tttttatagt attatcagta agtggagttc gtgaggctgt tgtaaatgcg
      661 ataccaaaaa atatgaaatt agctgttaca ggtggtatag gaatatttat tgcacttata
      721 ggtctagtta acagtggaat tgttattgga gaccaagcca ctcttatcaa aatgggtaga
      781 tttacaccag cagtaataat aacttgtgta ggattaataa ttattgctgt attagataaa
      841 aaaagagtta aaggttctat actttttgga atagtagtga gttcattact tgcttgggca
      901 tttgctttta taaatccaga gtatgcacaa aaattaggta tatatttacc tggtggatta
      961 tttaaattcg aaagtttagc accaatagca ggtaaaatag atttaggata tgtactacat
     1021 ccaacaaata taggtggttt tttagtagta gtttgtacat ttttatttgt tgattttttt
     1081 gatacagtag gtacattagt tggtgtttgt tctaaagcaa acatgttaga tgaaaaaggg
     1141 aatgttccta atgtaggtag agccttactt acagatgcgg ttgcaactac agttggtgca
     1201 ggacttggag tatctacagt tacaacttat gttgagagtt caacaggagt tatcgcaggt
     1261 ggaagaactg gatggacagc aataacagtt ggaattttat tcttagctgc aatgtttttc
     1321 tctcctgtat ttatagcaat tccatcatgt gctacagcac cagctttaat atatgttgga
     1381 tatttaatgc ttggtacagt taaggatata gagtttgata atataactga aggtgttcca
     1441 gcttttgtta ctattgcttg tatggcatta acatatagta taggtgatgg tttaacttta
     1501 ggtatattaa cttatgtttt cgtaaatata ttttataata tatttggagc taaaaaggca
     1561 gaagacaaaa aacatgtttc tgtagtaatg attgttctgg caatattatt tataataaaa
     1621 ctttggctac tataataggt attatttaaa attttaattt aatataaaat aaagggctgt
     1681 aatttattat caattgtgat gataaactac agctcttttg tatttgttgt atttttgtat
     1741 ttattgtatt atttaataat gtctattagt tctccatttt gtaaagtttt aggtgtaatt
     1801 actatattga actctttttg caaatctttt ataatatcta attctttttt tgttattatt
     1861 gagattgtgt taccacttcg atttcctcta gcagttcgac cacatctatg caggtattca
     1921 tttttacttt ttggaaaatc caagttaaaa acatgactta catccacaat atctagtcca
     1981 cgagcagata aatcagttgt aattaaaatc tttgctttac caagtttaaa tttatttatt
     2041 gcattctttc tatcttcttt ttccatattt ccaaaaattc ctattgcttt gtaattatga
     2101 taatttagtt ttgatactat gacttctata tttttttcat tatttacaaa tacaatagct
     2161 ttttttggat ttgtagccgc taatgccttt ctaagaaaag taaatttatc ccttatctca
     2221 cctaataaat atgagtgatt gatgttagga tttatttgtg atttttcctt tgtttttatt
     2281 atttcaaact ccttcataag tttatcacaa atttttatag tgctatcagt aagacttgca
     2341 gagcatccta taatttgtct atctcttaac gttgttctta ttatgtcttc tatacaagtt
     2401 atattttttc cattcaatag attgtccact tcatctaaga caatagtttt tatattatgt
     2461 gattttagct tcttttgttt tatcaaatct aaaactcttc cacaagagcc aattactatg
     2521 tgtggtttta ctgcttttat attttttatt tgtttttgta tattgacctc cccaattaaa
     2581 gctaaagaag taacggagag ttctgcattt ttagccaata attcaacctg gtttgtaatc
     2641 tgcattacta attcatgcgt aggtgcaagt ataagagctt gtgtttctct ttttgatgta
     2701 tcaatttttt caaatatagg taaaaggtat gctagtgttt ttcctgttcc agtctgtgaa
     2761 ttaatcaata aatccttatt ttgaattata ttccctataa caagtgactg aacttctgtt
     2821 ggagaagtta tgtcttgttt tttgagtcca tctattagag tggaacttat ttttaattgt
     2881 tcaaatgtat tcatatttta ctccttatat tttaataatt atagtcaact attttttatt
     2941 atacattgtt tattcaaatc gaactatata ttcttttata attatgtgta atttgaaagt
     3001 aaatatcaat tatatttata cctttatatt tctgatacct ttgcatttta tagatttcta
     3061 aaataaacaa ataaaagttg taattgtttg atatttgtta tatattatat ataacaaata
     3121 tcaaaactat aatatggagg gaaattatgt taaaagtaat caatgtatct aaatcatata
     3181 aaaataaaaa agtagtaagt agtgtctcat ttgatgtaaa tgatggagag ctttttgggt
     3241 ttataggaca taatggagca ggtaaaacta ctactattaa agcaattgtt ggaatacacg
     3301 attttgaaga gggagaggta ctaataaatt ctaaatctat aaaaaaacag cctgttgaat
     3361 gtaaaaagga aatggcatat atacctgata atccagattt atacgagtca ttaactggta
     3421 tacaatacct taattttata gctgacatat ttgaagttgc caaagataaa agagaagagt
     3481 taataaaata ttattcaaat aagtttgaac tcaataaagc attaggagat ttaatatctt
     3541 catattcaca tggtatgaaa caaaaattag ctataatttc ggctttgata cacagcccaa
     3601 aaatcttaat tcttgatgaa ccttttgtag gtcttgaccc aaaagcttcc tttatattga
     3661 aggaaattat gagggagttt tgtgacaaag gtggttgtat atttttctca acacacgttt
     3721 tagaagttgc tgaaaaaatt tgtgataaga ttgccataat aaaagatgga aatataattg
     3781 cttatggaac tacagaagaa gttaagggaa gtaattcact tgaaagtatt ttcatggaat
     3841 taatagagaa ataaaatgtt ttttatacaa tatagtgtct aaagtgagga atgataaaat
     3901 atgagcaatc taagtatatt aataaaaaat aatataataa atgaatttaa attaaatgcg
     3961 ttaaaaaaag ctgatggtag agaaaaacaa aaaataatgg gaatgatgct ttcagttgtt
     4021 ataatagcgg gtctattaat tttttatgta acgagcttaa gtctctcttt agttgatatc
     4081 ttaaagcaaa taaatcaact ggaaatgcta ctgatatttg gttttggatt atcaacatta
     4141 acaactattg ttacatcatt atataaaacg tcatcatatc tatttcaagc aaaagattta
     4201 gaccttttga ctagtttacc tataaaagaa tctactgtac tagcaagtaa gatattaatg
     4261 ttggttggag caaactattt attttcttca atatgtatga ttatacctgc agtggtttac
     4321 tttttaaatt ctgatgtaag tataacttat ttcccttatt tgatattgct atttttagct
     4381 ttaccattat taccgatagt tgcttcatca ataatttttc tatttatagg gcagatatca
     4441 tcaagaataa aatacaaaaa tttagtatta atcataggta gcattatatt gatgttgctt
     4501 tataccctac ttatgtcaag acttaattct atgtcagttg aaatgctaaa gaatagtgtg
     4561 tctatttcag agacgattag aaaagtatat cctcctatat attattttgt tgatgctctt
     4621 aaaacaggaa gtatagtttc atttttagca tttatagcca tctcaataat accatttttt
     4681 atgtttgtaa ctctgtttgc aaaaaaattt aaagatataa atgctaagat gagtgaagga
     4741 tataagtcaa aaaattatga atttaagggt caaaaaactt caagaccttc aaaagcttta
     4801 ttaaaaaaag aatttaagag atatacttct tcgtttatat actttttaaa ttcatctgca
     4861 ggtttgttat ttctaatagt aggtacaatt ttaatattat tatttggagc agataaagtg
     4921 ggtgagttac tgaaattgaa tatagatttt aatcttataa aaatacaatt attgggagta
     4981 ttcttcttta taatagtaat gaattgtaca acatactgtt caatttctct tgaaggaaaa
     5041 aacttatgga tactgaaatc ttctccaata gatgaaagag aaatattctg gtcaaaaata
     5101 ttgatgaatt ttttattgaa tgcaccacta tctattttat gtctaatatt agtatctata
     5161 agattagact ttgaaattca gtttattatt gtatcaatct gtttgattgg aagtttttca
     5221 atgtttgtag cattgatggg attattatca aatttattat acccaaacct tgattggaaa
     5281 aatgaggtgg ctgtagtaaa aagaagtgca agtatgatag ttacaatgtt agtatcatta
     5341 gcttatgtaa ttatacttgg aggtatatat gtattacttg gaatttcgtt tttaaatata
     5401 tatattattt tggcaagtat acttactttg atattagatt ttatgatatg gagaattatt
     5461 tctactaaag gagtaaaaat atttaaaaat ttataagtgt atttatgtca aataaagact
     5521 tatctttttt agtct
//
LOCUS       DAELLV010000222         5509 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.222, whole genome shotgun sequence.
ACCESSION   DAELLV010000222 DAELLV010000000
VERSION     DAELLV010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5509
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.222"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            1104..1334
                     /locus_tag="KPD58_003116"
     CDS             1104..1334
                     /locus_tag="KPD58_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG0266847.1"
                     /translation="MSNKKKKYANILANECVACGSCIKACPRSAISVPCGISAKINRD
                     LCVGCGICEKICPASVIEIITILKEEEGKCHE"
     gene            1327..1908
                     /locus_tag="KPD58_003117"
     CDS             1327..1908
                     /locus_tag="KPD58_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG0266848.1"
                     /translation="MNNKKHWYDYLWIFSSIYLILGFVNIIFAWIGLICFFVPLAISI
                     VKGNKAYCNKYCGRGQLFNLLGNKLKLSRNRDIPKFIRSKWFRYGFLTFFMVMFANML
                     FSTYLVFEGSRNFKEVVTLLWTIKMPWQWAYHGTLVSPWVVQFAFGFYSVMLTSTVLG
                     LITMILFKPRSWCVYCPMGTMTQMICKAKSKSV"
     gene            complement(2486..3163)
                     /locus_tag="KPD58_003118"
     CDS             complement(2486..3163)
                     /locus_tag="KPD58_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG0266849.1"
                     /translation="MISQNEIDILTHSLPFWDKLTDLQKELLISSANTSHYKKGNPVH
                     CGDSDCIGILIIKSGTVRTYILSDEGREVTLFRLDDGDVCILSASCILKTITFDVYVD
                     AETDCDIIQISSSVFAKLSTENIHAELFSYKLATERFSDVMWAMQQILFMSFDKRLAS
                     FLIDEIAKNGSNTINMTHEQIAKYMGSAREVVSRMLKYFAREGIVSLSRGGIKVLDKD
                     KLRSLTL"
     gene            complement(3352..4716)
                     /locus_tag="KPD58_003119"
     CDS             complement(3352..4716)
                     /locus_tag="KPD58_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG0266850.1"
                     /translation="MKESDKRIELLGSAPISKALLSMGLPTMIGMMITALYNLVDAYF
                     VGGLGTSQMGAISVAFPLGQLVVGLGLLFGNGAASYIARLLGHGDNEKAGKVVSTSIY
                     SSIIVGTLVIIVIMIFLNPILKLLGATKSIMPYAISYASIYVVSSIFNIFNVTMTNIV
                     TSEGAAKTTMCAMLLGALLNMILDPIFIYTFGLGVRGASIATAISQIASTLVYVGYIL
                     SKKSIFSFRINECCFSKEIFSEILKIGIPTLIFQLLTSLAITLTNMEATKYGDSVIAG
                     MGAVTRITSLGSLVVFGFIKGFQPIAGYNFGSKNYDRLHIAIKTSILWTTVFCVVLGL
                     ILILFPKSIISQFTSGDILLIENGSKALRANGFSFMLFGFYTVYSTLFLALGKAKECG
                     FLGMCRQGVCFIPIILIIPVFYGLNGILYAQPIADLMAAIIAGFMSIRLHKELNLLKI
                     NGMH"
     gene            4844..5365
                     /locus_tag="KPD58_003120"
     CDS             4844..5365
                     /locus_tag="KPD58_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBG0266851.1"
                     /translation="MATIDLIVLGIVKQQPKSAYDIQKAVEYRNISRWVKISTPSIYK
                     KVIQLEQKGYIESKTVKNGKMPEKAVYSLTELGETHFLDLMFDTSSKPINILLDFNAV
                     IVNLNSLTPEKQKLCLNNIEENVQELKKTIEENISIKTEVPETAKAVLEQQLVLAQAL
                     ETWINSLKDNMGN"
BASE COUNT         2100 a          693 c          900 g         1816 t
ORIGIN      
        1 gtttaacttg tggttaatag agtaaggtgt aactaaagat aaacttcaat atgggtttaa
       61 cttgtgatta atagagtaag gtgcaactaa agataaactt caatatgagc ttaacctatg
      121 attaatagag taagaatgta aatagagata aacttcaata tgggcttaac ttgtggttaa
      181 tagagtaaga gtgtaactaa agataaactt caatatgagc ttaacctatg attaatagag
      241 taagagtgta aatagagata aacttcaata tgggtttaac ttgtgattaa tagagtaggg
      301 tgtaactaaa gataaacttc aatatgagtt taacttgtga ttaatagagt aaggtgcaac
      361 taaagataaa cttcaatatg agcttaacct atgattaata gagtaagaat gtaaatagag
      421 ataaacttca atatgggctt aacttgtggt taatagagta agagtgtaac taaagataaa
      481 cttcaatatg ggtttaactt gtggttaata gagtaagaat gtaaatagag ataaacttca
      541 atatgagctt aacttgtgat taatagagta agagtgtaaa tagagataaa cttcaatatg
      601 ggattaactt gtgattaata gagtaagagt gtaactaaag ataaacttca atatgagctt
      661 aacctatgat taatagagta agaatgtaaa tagagataaa cttcaatatg ggtttaacct
      721 gtggttaata gagtagggtg taactaaaga taaacttcaa tatgagttta acttgtggct
      781 aatagaataa gattgtgaat aaagttatag ttttagatgg gtattaacct gttactaata
      841 gaatatgatt aggtgtaaaa ttatagagct gtataagaaa atattataga tatattaaat
      901 attagataat aaatatttat atagatacac aaaagaaaag gcgatatgaa gctaaactta
      961 atgacttcat atcgtttttt atttgaaact cctttgtatt tagagtgtga tttttaatat
     1021 tgaggtttta tgtgattaag tcacagataa aaagtaagta tcttgatata attcttatag
     1081 attaataata tagggaggtt attatgtcta ataaaaaaaa gaagtatgca aatattttag
     1141 caaatgagtg cgttgcttgt ggtagctgta taaaagcatg tccaagaagt gctatctcag
     1201 ttccatgtgg cattagtgca aaaattaata gagatttatg tgttggatgt gggatttgtg
     1261 aaaagatatg tccagcatct gtaattgaga ttataacaat attaaaagag gaagaaggta
     1321 agtgtcatga ataataaaaa acattggtat gactatttat ggattttttc ttcaatatat
     1381 ttgatattgg gatttgtaaa tattattttc gcttggattg ggctaatttg tttctttgtt
     1441 ccactagcta tttctattgt taagggaaat aaagcttact gtaataagta ttgtggaaga
     1501 gggcaactgt ttaatttgct tggaaataaa ttgaagctgt caagaaatag agatattcct
     1561 aaatttatta gaagtaagtg gtttagatat gggtttttga cattttttat ggtaatgttt
     1621 gctaatatgc ttttttcaac ttatttagtg tttgaaggaa gtagaaattt taaagaagta
     1681 gtgactttgt tgtggactat aaaaatgcct tggcaatggg cgtatcatgg aactttagtt
     1741 tcaccttggg tggtacagtt tgcatttggt ttttatagtg ttatgcttac atcaactgtt
     1801 cttggtttga ttactatgat attgtttaaa cctcgttcat ggtgtgttta ttgccctatg
     1861 ggtactatga ctcagatgat atgtaaggca aagagtaagt ctgtatagaa aggccttatg
     1921 tgatatttac agttttgttc aagtagagat gagtagtagg tttgaatata tatgacaaat
     1981 atatgagtat aggggcatag atttagtttg tgtaaggcaa actgatttat gcttcttttt
     2041 tatgtcttag attattggct aagtagaaac aatgaattta gatattaaga attattcagt
     2101 tatttgaaga aaatagaata ataattaaca ttatatgaat ggatattcat tacatataag
     2161 cagttaatag caagatttat ttaatatgaa ataggtcaag tatttaaact aaataaggtc
     2221 atgacaatta atataagtat agatataaaa agattagaag ttataactta taaaaggtat
     2281 tctatataag tagtatgtgg aaaatagtca aattaactta ttagaaacaa aaaatataca
     2341 taaaaaataa atgtaaatat caaataagat actatataaa aatagatata agtatctttt
     2401 ttattattga aattatgaaa atatatttta ttttacacaa aaaagagtgc ctcatgaact
     2461 aaaaagttca ttttgagaca cacttttaca gtgtcaaaga acgaagttta tccttatcca
     2521 atacttttat accaccacga gataaagaca caataccttc tctagcaaaa tatttcaaca
     2581 ttctcgatac aacttctctg gcactaccca tatacttagc aatttgttca tgagtcatat
     2641 ttatagtgtt actgccattt ttagctatct catcaattaa aaaagatgcc aaacgcttat
     2701 caaaactcat aaaaagaatt tgttgcatag cccacataac atcagaaaaa cgttcggtag
     2761 ctaatttata tgaaaataac tctgcatgaa tattttctgt agataatttt gcaaaaacag
     2821 aggaactaat ctgtataata tcgcagtccg tttcagcatc tacatagaca tcaaaagtta
     2881 ttgtttttag aatacaggaa gctgataaaa tacatacatc tccatcatca agtcggaaca
     2941 gtgtaacctc tcgaccttca tctgaaagta tgtatgtacg cactgttcca gattttataa
     3001 tcaaaatacc aatacagtca ctatcaccac aatgtactgg attccctttt ttgtaatgcg
     3061 aggtatttgc tgaagaaatt aataattctt tttgtaagtc agtaagttta tcccaaaaag
     3121 gcaagctatg tgtaagtata tcaatttcat tttgtgaaat catacaatat ctcctttctt
     3181 aaattgttat aatcccatga tacaagtgaa tagataaatt gacaagtagt ttgttaagag
     3241 gattatttag tcagatagta atagtatgat ttatgtagaa aaaatatgat aatatatatt
     3301 cttgctatta atgagaaagt aatatgtttt gatgatagta ggtttgtata actaatgcat
     3361 accgtttatt tttaaaagat taagttcctt atgtaacctt atagacataa aaccagcaat
     3421 tatagcagcc atcaaatcag ctattggttg tgcatataag ataccattta acccatagaa
     3481 tacaggaata atcaaaatta tagggataaa acagacacct tgtctacaca tacctaaaaa
     3541 tccacattcc tttgcttttc ctagtgcgag gaataatgta gaatacactg tatagaatcc
     3601 aaatagcata aatgaaaacc catttgccct aagagcttta ctaccatttt ctattaataa
     3661 aatgtcacca cttgtaaatt gtgaaattat agattttggg aagagtatca atataagacc
     3721 aagcactaca caaaatactg tagtccataa tatggaagtt tttattgcta tatgtaatct
     3781 atcgtagttc tttgaaccaa aattataacc agcaattggt tggaaacctt ttataaaacc
     3841 aaatacaaca agactaccta aagatgttat tcgtgttaca gcacccattc cagctatcac
     3901 agaatcacca tactttgtag cctccatatt agttaatgta atggcaagac tagtcaataa
     3961 ttgaaatatt aaggtaggta tgccaatttt taggatttct gaaaaaattt cttttgaaaa
     4021 acagcattcg ttaattctaa aactaaatat acttttcttg cttaaaatat atcctacata
     4081 aacaagtgtt gaagcaatct gtgagatagc tgttgcaata gaagcacccc ttacccccag
     4141 accaaaagta taaatgaata tcgggtctaa aatcatatta agcaaagcac ctaataacat
     4201 agcacacata gttgttttag cagctccttc acttgtgaca atattagtca tagtgacatt
     4261 gaatatatta aaaatagatg acacgacata aatgctagca taagatattg cgtaaggcat
     4321 aatacttttt gtagctccta acaattttaa aattggattg agaaaaatca taatcacaat
     4381 aataactaat gtacccacta taatactact gtaaatagat gtacttacca cttttcctgc
     4441 tttttcatta tcaccatggc ctaaaagtct ggcaatatat gaagcagctc catttccaaa
     4501 caataatcct aatcccacaa caagctgacc taatggaaat gcaacggata tagctcccat
     4561 ttgacttgtt ccaagtccac ccacaaagta tgcatcaacc aaattgtata atgcagttat
     4621 catcatacct atcatggtag gtaatcccat agataacaat gcttttgaga ttggggcaga
     4681 gcctaataat tcaattcttt tgtcactttc tttcatactt aacactcctc ttctattgac
     4741 attaagtcta aattataata ctataaatta tagtaagtat ggaaaatagt actataattt
     4801 atagtatttg tcaatagtaa gaaaactaaa ggagatgatt tttatggcta caatagattt
     4861 aattgtattg ggaatagtaa agcaacaacc caagagtgct tatgatattc aaaaggcagt
     4921 ggaatacagg aatatatcac gttgggttaa aataagtaca ccatcaattt ataaaaaagt
     4981 aattcaatta gaacaaaagg gatatattga aagtaaaact gtgaagaatg gaaaaatgcc
     5041 agaaaaagca gtttattcat taactgaatt aggtgagaca cattttttag atttaatgtt
     5101 tgatacatct agcaaaccaa taaatattct tttagatttt aatgctgtta tagtaaattt
     5161 aaatagcctt acaccagaaa aacagaaatt atgtctcaat aatattgaag agaatgttca
     5221 agagttaaag aaaactattg aggaaaatat aagtataaag acagaagtac cagagacagc
     5281 aaaagcagta ttggaacaac agttggtgct agcacaagca ttggaaacct ggataaattc
     5341 tttaaaagat aatatgggaa attagaatta gagaagctaa cttaataatc attaaacaaa
     5401 aaccagcata tttaataatg agatattaga aaaaataaaa caaatgatat aaactgaaaa
     5461 gtatatttaa aataaagctc acttaattga gggagaatgt taaactttt
//
LOCUS       DAELLV010000223         5478 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.223, whole genome shotgun sequence.
ACCESSION   DAELLV010000223 DAELLV010000000
VERSION     DAELLV010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5478
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.223"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            19..750
                     /locus_tag="KPD58_003121"
     CDS             19..750
                     /locus_tag="KPD58_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBG0266852.1"
                     /translation="MNIYAHRGFSGKYPENTILAFKKCLDMDIYGIELDVHRTKDGKI
                     VVIHDEKVDRTFNGHGFVKDFTLRKLKTLNSSFEGYQSNKECKIPTLEEVLILISPTD
                     LTLNIELKTDKINYPNIEKGVLELILKYNMKNRVLISSFNSNSLKNFHKLDPSVKTGL
                     LCYLPINNVVNFAKFLGNSYLHPPLVLVNESLIELCHKNLLGVNVYTVNEEDDILHCL
                     KLNVDGIFTNYPDIASNLLHSKQYS"
     gene            830..1465
                     /locus_tag="KPD58_003122"
     CDS             830..1465
                     /locus_tag="KPD58_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBG0266853.1"
                     /translation="MDNLLNELEALKSNLDNLPIEKIENYDLSKTALFIIDVNNGFAR
                     QGALYSPRVESLIKPIEMFTKKISNKLNRVIAFTDSHTPKSIELLSYPVHCLENDVES
                     ELVDELKSIENLQILPKNSTNGFFALENLDFDNIDNIIIVGDCTDICIYQFAITLKSY
                     FNQHNIEKNIVVPMNLVDTYDIPNVHPAEILNLVFFNSMIQNGVNVLKEIR"
     gene            complement(1716..2309)
                     /locus_tag="KPD58_003123"
     CDS             complement(1716..2309)
                     /locus_tag="KPD58_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3867 domain-containing protein"
                     /protein_id="HBG0266854.1"
                     /translation="MSDDRIIDFNELKNKVKDSDVDKFEQYIYNLYFSVMDGKMSMAE
                     FSRKIFDYMRDNNISQEKFMKIQKQFMERYGMDTEEVEKQLRNFGIDPSTAGFMSNNT
                     SSKVSTEDLESFKKSAGFYEKYGEKIQPKSCITTFIKNDLNDINVIIDQEKIMLCSDR
                     KINLMDSELNEFLLEYKNMFNKKIKVVMCETTNKYDY"
     gene            complement(2380..3243)
                     /locus_tag="KPD58_003124"
     CDS             complement(2380..3243)
                     /locus_tag="KPD58_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HBG0266855.1"
                     /translation="MLGIIAPSGPLRNTSLEEIKFNLESYGYEVKFSESCSLNYKGYL
                     AGNDDIRARDIEDMFLDKDVDIIMCLRGGYGTTRILDKINYDIIKQNPKPFIGFSDIT
                     GLNLAFYKNCGLLPYHGIMAANVGKWGKFTYKSLVNALEFKDELYLENPKEEKIYTVC
                     EGKAEGIIMGGNLSLIIATMGTKYEIDAKDKILFIEEIGEPQYKLDRMLTQLYSSGKL
                     EECNGIIFGDFKDCIEENDLMELLIEFANKVNKPSIYNLQSGHCIPMITIPLGRMCEL
                     DATEKIVKLKK"
     gene            3406..4575
                     /locus_tag="KPD58_003125"
     CDS             3406..4575
                     /locus_tag="KPD58_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0266856.1"
                     /translation="MLNIKTLCHEINDWVINIRRDLHKTPELGLEEFQTKKKIIKYLN
                     EIGINYIEYKNHTGITAYINVSPNFETVAIRADIDALPITEELNYSYKSINIGKMHAC
                     GHDAHTAILLGTCNILFKLKDYLNVNVKFFFQPAEETIGGAQLMIEDGCLEKPNVKYI
                     FGLHVNPNINKNLIELKYNTLNASTNTLQLTVHGSKCHGAYPHQGIDAIVISAHIITA
                     LQTIVSRNTNPTDSVVISLGKIEGGIKENIVCDKVVIRGTLRTLTPETREFSKKRIRE
                     ICDFTCKTFGGSISVEIEEGYPALINSNHLVDYVKQNAVELFGEENIILKDSPTLGAE
                     DFSYFLRHCEGAFYHLGCANREKNITSPLHTSTFDIDEDCLITGVMLHVKNVLSF"
     gene            4676..5152
                     /locus_tag="KPD58_003126"
     CDS             4676..5152
                     /locus_tag="KPD58_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBG0266857.1"
                     /translation="MKEILINPIGKICIQGEEVFIKLDKKYVPALNELDDFSHLNVFW
                     WADGFDNPELRSILETPKPYKSGPDVIGIFATRSPIRPNPIALTAVQIINIDHENGII
                     KIPYIDANDNSPVIDLKPYTPSVDRVENPSVPKWCSHWPKSVEKSGEFNWEDEIEF"
BASE COUNT         1959 a          807 c          681 g         2031 t
ORIGIN      
        1 taggagggaa ccatataaat gaatatttat gctcataggg ggtttagtgg aaagtatcca
       61 gaaaatacta ttctagcttt taaaaaatgt ttggatatgg atatatatgg aatagaactt
      121 gatgtccaca gaactaagga tggaaaaatt gtagttattc atgatgaaaa ggttgataga
      181 acttttaatg gacatggttt tgtaaaagac tttactttaa gaaaattaaa aactctaaac
      241 tcatcttttg aaggatatca aagcaataaa gaatgtaaaa ttccaacttt agaagaagta
      301 ttaattctaa tctcacctac agaccttact ttaaatatcg aactaaaaac tgacaaaata
      361 aactatccca atatagaaaa aggcgtttta gagcttattt taaagtataa tatgaaaaat
      421 agagttctta tatctagttt caacagcaat agcttaaaaa actttcataa attagaccct
      481 agtgtaaaaa caggattatt gtgttattta cctataaaca atgtagtcaa ttttgctaaa
      541 tttctgggta atagttatct tcatccacct ctagttttag taaatgaatc tctaattgaa
      601 ctttgtcaca aaaatctact tggtgtcaat gtatatactg taaatgaaga ggatgatata
      661 ctacactgtc taaaactcaa tgtagatggt atttttacta actatccaga tattgcttca
      721 aatcttttac actcaaaaca atacagctaa ttatacaata gtttgtactc cttgactttt
      781 atataattaa tatgtacaat tttattaaat ttatcttgga ggtataacta tggataactt
      841 attaaatgaa cttgaagctt taaagagtaa tttagataat ctaccaatag aaaaaataga
      901 aaattatgat ttgagtaaaa ctgctttatt tataattgat gttaataatg gctttgcaag
      961 acaaggtgca ttatactctc ctagagtaga gtctttaata aagccaattg aaatgtttac
     1021 aaaaaaaata tcaaacaaat taaatagagt aatagcattt actgattctc atactcctaa
     1081 atcaatagaa cttttgagtt atcctgttca ctgtttggaa aatgatgtag aaagtgaatt
     1141 ggttgatgaa cttaagtcga tagaaaacct acaaattctt cctaaaaatt ctactaatgg
     1201 attttttgca ttagaaaatt tggattttga taatatagat aatataatta tagttggcga
     1261 ttgcacagat atatgtatat atcaatttgc aataactcta aaatcatatt ttaatcaaca
     1321 taatattgaa aaaaatatag ttgtacctat gaatttagta gatacttatg atattcctaa
     1381 cgttcaccct gctgagattt taaacttagt atttttcaac agtatgattc aaaatggagt
     1441 taatgtatta aaagagattc gttaagcttc taatacttta catcccatat aatacaatta
     1501 tcagatattt atttttaata aaatttctaa tagaaaaaca cagttttagt gagaagatta
     1561 caatttaaaa cactatctat tattactaat cttcacaata taaaaaatcg cagtttagat
     1621 attccttata caaatttata aggaataata aattgcgatt tttattttta agtagtacaa
     1681 ttaatttata ctacttcagt atttaaattc attttttaat aatcatactt attcgtagtt
     1741 tcgcacatta ctacctttat ttttttatta aacatattct tatattctaa taaaaactca
     1801 tttagttctg aatccataag atttattttt ctatcactac aaagcattat cttttcttgg
     1861 tcgattatta cattaatatc atttaagtca ttttttataa atgttgttat acaactcttt
     1921 ggttggattt tttctccata tttctcataa aatcctgcac tcttcttaaa tgactctaaa
     1981 tcttctgtag atactttgct ggaagtatta ttactcataa aacctgctgt agaagggtcg
     2041 attccaaagt ttctaagttg tttttctact tcttcagtgt ccataccata tctctccata
     2101 aattgctttt gtattttcat aaatttttct tgagatatgt tgttatctct catataatcg
     2161 aatatttttc ttgaaaactc agccatagac atctttccat ccataacaga aaaatataag
     2221 ttatatatgt attgctcaaa tttatctaca tcagaatctt taactttgtt ctttagttcg
     2281 ttaaagtcta ttattctatc atcagacatt tttcatcctc ctagctaaat ttttacttgt
     2341 ataagattat accatatttc tattatattt atatacacac tattttttta atttaacgat
     2401 tttttctgta gcatctaatt cgcacattct gcccaaagga atagttatca ttgggataca
     2461 atgtcctgac tgaagattat atatagaagg tttattgacc ttatttgcaa attctattag
     2521 taattccatc aaatcatttt cttcaataca atccttaaaa tctccaaaaa ttataccatt
     2581 acattcctca agttttcctg aagaatatag ttgtgttaac attctatcca gtttatattg
     2641 aggttctcca atttcctcaa taaacaaaat tttatctttt gcatctattt catattttgt
     2701 tcccattgtg gcaattatta aggataaatt tcctcccatt attattccct ctgctttacc
     2761 ttcacataca gtataaatct tttcttcttt tggattttct aaatataatt catctttaaa
     2821 ttctaaagca tttaccaatg atttataggt aaatttaccc catttaccta catttgctgc
     2881 cattattcca tgatatggta aaagaccaca atttttatag aaagctaaat ttaaacctgt
     2941 tatatcagaa aaacctataa atggttttgg attttgcttt attatatcat aatttatttt
     3001 atccaatatt cttgttgttc cataacctcc tcttaagcac ataattatat ctacatcttt
     3061 atctaaaaac atatcttcta tatcccttgc tctaatatca tcattaccag ctaaataacc
     3121 tttataattt aaactacaac tttcagaaaa cttaacttcg taaccataac tttctaaatt
     3181 aaactttatt tcctccaaac ttgtatttct taatggccca gatggcgcaa ttattcctaa
     3241 catttaaaca cctcttctta taaaatgata tattcttctc catactaaat atctctaata
     3301 taattataag atattttatt tgtacttgat acataataat gatttacata aaataatata
     3361 attaatgaag aaaaataaat ttttaatata gttaggatgt gttttatgct taatataaaa
     3421 actctttgtc atgagataaa tgattgggtt ataaatataa gaagagattt acataagact
     3481 ccagaacttg gattagaaga atttcaaact aagaaaaaaa ttattaaata cttaaatgaa
     3541 ataggtataa attatattga atataagaat catacaggga taactgcata cattaacgtg
     3601 agtccaaact ttgaaacagt tgctataaga gctgatatag atgccttacc tataacagaa
     3661 gaacttaatt attcttataa atctattaat attggcaaaa tgcatgcatg tggtcatgat
     3721 gcacacactg ctattttact gggtacttgt aatatactat ttaaattaaa ggattactta
     3781 aatgtaaatg taaaattctt ttttcaacct gctgaggaaa ctattggtgg ggctcaactt
     3841 atgatagaag atggatgtct tgaaaaacca aatgtaaaat atatatttgg actccatgta
     3901 aatcccaata tcaacaaaaa tctaattgaa ttaaaataca acactctaaa tgcttccacc
     3961 aatacacttc aattaactgt acatggttca aaatgtcatg gagcttatcc acatcaaggt
     4021 attgatgcta ttgttatatc tgctcatatt attacagctc tacagacaat cgttagtcga
     4081 aacacaaatc caactgattc tgtggttatt tctctaggaa agattgaagg tggaatcaaa
     4141 gaaaatatag tttgtgacaa agtggttata aggggtactt tgagaacctt gactcctgaa
     4201 actagagagt tttcaaaaaa acgaattaga gaaatttgcg attttacttg taaaacattt
     4261 ggaggaagta tttctgttga aattgaggaa ggttatccag ctcttatcaa ttcaaaccat
     4321 ctagtagatt acgtcaaaca aaatgctgta gaattgtttg gtgaagaaaa tattatcctt
     4381 aaagactcgc ccactcttgg tgctgaagac ttttcgtatt ttctaagaca ctgtgaagga
     4441 gctttttacc accttggttg tgcaaatcgt gaaaaaaaca ttacttcccc tttgcatacc
     4501 tctacttttg atattgacga agattgcctt attactggtg tcatgctcca tgtaaaaaat
     4561 gtattatcat tctaactatt aatattttta tttattattt tatattgacc ctcacatgat
     4621 gtcatgttat accctaaaat tacaaatgat aaattaaata gttggaggta atattatgaa
     4681 agaaatatta ataaatccta taggtaaaat atgtattcaa ggcgaagagg tctttattaa
     4741 attagacaaa aagtatgtac ctgcattaaa tgaactcgac gatttcagtc atttaaatgt
     4801 attttggtgg gctgatgggt ttgataatcc tgaactaaga agtattttag aaaccccaaa
     4861 accttataaa agtggaccag acgttatagg aatatttgca acaaggtcac ctataagacc
     4921 caatccaata gcattgactg ctgtacaaat tatcaatatc gaccatgaaa atggcataat
     4981 aaaaatacca tatattgatg ctaatgataa tagtcctgtt attgacttaa agccatatac
     5041 tccaagcgtt gatagggttg aaaatccgtc cgttcctaaa tggtgtagtc actggcctaa
     5101 gagtgtagaa aaatcaggag aatttaattg ggaagatgaa attgaatttt aaattacttt
     5161 tttcttgatt tcattaactt gttttccaca tttattttat ttacacttac caagatttta
     5221 tgtcacatat acatataata ttaggctgta ttatataata caacctaata ttatataata
     5281 aatttctatt tttcccttaa atcattcaca ttctttctaa gctacataag atttttctat
     5341 tatttctttt tgagtttaat ttagcattat gttgaggaaa aaacatttca tgcattttaa
     5401 taaattaaat ttttctttta ttctgcgatt ttataataat caatttaact taaaaataat
     5461 acctaactat tttaacta
//
LOCUS       DAELLV010000224         5442 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.224, whole genome shotgun sequence.
ACCESSION   DAELLV010000224 DAELLV010000000
VERSION     DAELLV010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5442
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.224"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(391..2670)
                     /locus_tag="KPD58_003127"
     CDS             complement(391..2670)
                     /locus_tag="KPD58_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG0266858.1"
                     /translation="MLSDAKNNRYGLSIIAVISILVITISCLIYGSRLNKTLGEETNQ
                     YLSEIANQSVNVLKKQINGDIKLLESISIFIEGEESFEVDNILSILKRQAINSSFKRM
                     GVILPNGTAYTTDGYIEDFSKRDYFLKSMQGEVVITGKLKDVIEDDKNTNSNINVYSV
                     PIYKDNEVKGVIFATHSTKLYEKILSVPTFNGKGYSYITRSNGDIVLNPSSKNANSTK
                     ENLFSYIGRSDSISKSSINNMKSNIKNFKSGSLSYSMFGKGYYLSYAPIGINDWYLFS
                     EVPKTAISEKSYAIIKLTLSACVVLIIIFTALTIYILFIHKKSKEKLEEFAFKDNVTG
                     IGNSNKFNLEGGKFLSSHEKKNLVLIYFDIDKFKLVNDRFGYEEGDRVLKEIAEIIKN
                     IFKEQSVFSRISNDNFAIIFEKKNNRESIIEICELIRKKLSMIKTSLGVELNLIPSIG
                     VYFVEEGETNISTCLDKAMIAKTTVKRKYLKVYEIYAENLKETLIEERDIEQEMHDAL
                     KNEQFKVYLQPKVDLSTTKIVGAEALVRWQHPKKGLISPGVFIPIFEKNGFITELDMF
                     VFTQVCKNFKRWENENFPTFPISINLSRVHLENPGFISELERITKEYEIEPNLIEIEL
                     TESAIFDNTKILFKIMQTLKSVGFKISMDDFGSGYSSLNMLKDMPIDVLKLDRQFFIT
                     VGNAKKSQIVVSSIVQMAKQLDIKVVSEGVETVEQAEFLRFIGCDMAQGFLFARPMPI
                     EEYEKLILDRYSLNRKETN"
     gene            complement(2941..4395)
                     /locus_tag="KPD58_003128"
     CDS             complement(2941..4395)
                     /locus_tag="KPD58_003128"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HBG0266859.1"
                     /translation="MKLNDIIQGLDIINVKGELNIDINNVQYDSRKVTKGTLFICIKG
                     FVSDGHKYIKDAIEKGASAFLVEEDVAIKGCTFIKVKDTRKDMAKVADNFYNHPSQKF
                     NVIGVTGTNGKTSITTILNEILTLNKNKVGLIGTIKIFDGEKDIVSNSTTPESIDLQY
                     HFNNMLDNGCDYCAMEVSSHSLALNRVDETDFKLGIFTNLTPDHLDFHKDLEDYRKAK
                     EKLFFKTTMANIINIDDEGGKKIYENIKGINVPCYTYGVDTKADFMARDIKSDSDGVS
                     YRLITPSYEEVIFIPVPGMFTVYNTLAVIAACYVLGIPKPIYKEGLRLSNGVSGRFET
                     VPNDKGISVIVDYAHTPDALENVLKTTQQFAEGKIISVFGCGGDRDTEKRPLMGAIGQ
                     KYSDLCIITSDNPRTEEPEAIIKDILEGIDKKKENYHVVVDREQAIAEAISMAKKDDV
                     VIITGKGHETYQIIGKVKHHFDDKEVANECLSKM"
     gene            4559..5428
                     /locus_tag="KPD58_003129"
     CDS             4559..5428
                     /locus_tag="KPD58_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0266860.1"
                     /translation="MDNKIGYLNDNFKIFNIRDKKDIQFEYHNHDFNKIIIFINGNVT
                     YFVDGIPYKLKPWDILFISNNEIHKPVIDSNVFYERIAIWISPEFMKKNSDATSDLEK
                     CFKIATENKCNRLRLNIHDIESIKSFIEQINISESNTEFGNEILRNALFIQLMIFLNR
                     IFLKNENMESTEDVFYDETIKKVLNYINSNIDDKLSIDSISSEFFISKYYLMRKFKSH
                     TGTSIHNYIVQKRLILAKSLITNGHLMSEVCNKCGFNDYSSFVRAFKKNYGVSPKNYI
                     NNKNPLDITLLDE"
BASE COUNT         1876 a          842 c          571 g         2153 t
ORIGIN      
        1 atagaattta aaaataaaag aagagtattt caaatgattt gtataaatta ttatgaaaaa
       61 ctcttttttt gatataataa aattacttac atatataaca tttaaattta taaattatct
      121 ttttattttt taaaatatgt ataacacacc tatagaaatt actattatta ctatctaaag
      181 tattttgcta taatcccata tactcttacc tcaaaaacat ccaaatgtaa aaaatactac
      241 atattaataa actttgccca ataattccta aaataatatc ctaacaatga tttatgtttt
      301 tataatagta tatttttaca taaaaaagtg aacattgatt aacaaagttc acttttttat
      361 aaatttttat gttataattc acaattttat ttaatttgtt tcttttctat ttaaactata
      421 tctatctaat attaattttt catattcttc tattggcatt ggtctagcaa ataaaaatcc
      481 ttgggccata tcacaaccta taaaccttaa aaactctgct tgctctacag tttcaacacc
      541 ttctgaaaca acttttatat caagttgctt tgccatttga acaatacttg aaactacaat
      601 ttgactcttt tttgcatttc ccactgttat aaagaattgc ctatctaatt tcaatacatc
      661 aattggcata tctttaagca tatttaaaga agaataacca gaaccaaaat catccataga
      721 aattttaaat ccaacagatt tcaaagtttg cattatttta aataatattt ttgtattgtc
      781 aaatattgca ctttctgtca attcaatctc aattaaattt ggttcaattt catattcttt
      841 tgttattctt tcaagctcag aaataaagcc tggattctca agatgtactc ttgataagtt
      901 tattgatata ggaaatgtgg ggaaattttc attttcccaa cgtttaaaat ttttacaaac
      961 ttgggtaaat acaaacatat ccaattctgt tataaaacca tttttctcaa aaataggaat
     1021 aaatacacct ggacttatta gacctttttt aggatgttgc caacgtacaa gtgcttcagc
     1081 acctacaatt ttagtagttg ataaatcaac cttaggctgt aaataaactt taaattgttc
     1141 attttttaaa gcatcatgca tttcttgttc aatatcacgt tcttctatga gagtttcttt
     1201 taaattttca gcataaatct cataaacttt taaatatttt ctttttactg tagtttttgc
     1261 aatcatagct ttgtcaagac aggtagaaat atttgtttct ccctcttcaa caaaatatac
     1321 acctatggat ggaattaaat tgagttctac tcccaaactt gtttttatca ttgacaattt
     1381 tttacgtata agttcacata tttctataat tgactctcta ttatttttct tttcaaatat
     1441 tatagcaaaa ttgtcatttg aaattcttga aaatacagat tgctctttaa atatattctt
     1501 aattatctct gctatttcct ttaatactct atctccttct tcatacccaa acctatcatt
     1561 tactagctta aacttatcta tatcaaaata aattaaaact agattctttt tctcatgaga
     1621 ggataaaaat tttccccctt caagattaaa tttatttgaa ttgccaatac ctgtaacgtt
     1681 atctttaaaa gcaaattctt ccaatttttc cttactcttt ttatgtataa atagtatata
     1741 aattgttaat gccgtaaaga ttataatcaa gactacacat gctgataatg taagttttat
     1801 aatagcatat gatttctcag atatagcagt tttaggaact tcagaaaaaa gataccaatc
     1861 attgattcct attggggcat aacttaaata atatcctttt ccaaacatac tataacttaa
     1921 tgaacctgat ttaaagtttt ttatatttga cttcatattg ttgatactag attttgatat
     1981 tgaatcactt ctgccaatgt aagaaaataa attttcttta gtagagttgg cgtttttact
     2041 gcttggattt aaaacaatat caccattact tctagttata taagaatatc cttttccatt
     2101 aaatgtaggt acagaaagta ttttttcata caacttcgta ctatgtgtgg caaatataac
     2161 tcctttaacc tcattatctt tataaattgg tacagaatat acattaatat tactattggt
     2221 atttttatca tcttctatta catcttttaa tttgcctgtt ataaccactt ctccttgcat
     2281 tgattttaag aaataatctc ttttagaaaa gtcttctata taaccatctg tagtgtatgc
     2341 tgtaccattt ggtaaaataa ctcccattct tttaaatgaa ctattaatag cttgcctttt
     2401 tagtatagaa aggatattat ctacttcaaa agactcttct ccttcaataa atattgaaat
     2461 tgactctagt aactttatat ctccattaat ttgttttttt aacacattga cgctttgatt
     2521 tgctatttct gaaagatatt gattcgtttc ttcacctaat gtcttattca accttgaacc
     2581 ataaatcaaa caggaaattg taattactaa gatacttatg acagcaatta tggacaaacc
     2641 atacctatta ttttttgcat cacttaacat tatgcttcct gcctttctcg aatctatgta
     2701 tgtataatca ttatcttatg acaattattg taaacatttt ataataatac ttatttttcc
     2761 atatttactg taattataca aaattataaa ctttattaca acaataaatt aattaatata
     2821 gatattagtg tttatattat tgcattatct attttacaat tatttacata taaaaaaatg
     2881 taatatcaaa gtgaagcttg atattacatt tttgttcttg tattttgttt aatttttatg
     2941 ctacatctta cttaaacact catttgcaac ttctttatca tcaaaatgat gcttaacttt
     3001 acctataatt tgataagttt catgtccctt acctgttata attacaacat catccttttt
     3061 tgccatactt atagcttctg ctatagcttg ttctctatca acaacaacat ggtaattttc
     3121 ttttttcttg tcaattcctt caagtatatc ttttattatt gcctctggtt cttcagttct
     3181 tggattatca gaagttataa tacataaatc agaatatttt tgtccaattg ctcccatcaa
     3241 aggtctcttt tcagtatctc tatctccacc acacccaaac acagaaatta tttttccttc
     3301 tgcaaattgt tgagttgtct ttagtacatt ttcaagtgcg tcaggagtat gagcatagtc
     3361 tacaattacg cttatacctt tatcatttgg aactgtttca aaccttcctg atactccatt
     3421 agaaagtcta aggccttctt tatatattgg tttaggtatg ccaagtacat agcaagcagc
     3481 tataactgct aaagtattat atactgtaaa cattcctgga actggtataa atattacttc
     3541 ttcatatgaa ggtgttatta atctataaga tactccatcc gaatctgatt ttatatctct
     3601 agccataaaa tcagccttag tatcaacacc ataagtgtaa caaggtacat ttataccttt
     3661 tatattttca tatatcttct ttccaccttc atcatctatg tttattatat ttgccatagt
     3721 agtcttaaaa aataattttt cttttgcttt tctataatcc tctaaatctt tatgaaaatc
     3781 taaatggtct ggtgttaaat ttgtaaatat gcctagttta aagtctgttt catcaactct
     3841 atttaatgcc aatgaatgtg atgaaacttc cattgcacag tagtcacatc cattatccaa
     3901 catattatta aaatgatatt gtaaatctat actttctgga gttgttgaat tggaaacaat
     3961 gtctttttct ccatcaaaaa tttttatagt tcctataagt ccaactttat tcttatttaa
     4021 agttaatatc tcatttagta tagtagttat actagttttt ccattagtac ctgtaactcc
     4081 tataacgtta aatttttgag atggatgatt gtaaaaatta tcagctactt ttgccatatc
     4141 ttttcttgta tcttttacct ttataaatgt acatcctttt attgctacat cttcttcaac
     4201 aagaaatgca cttgctccct tttctatggc atcttttata tacttatgac catctgacac
     4261 aaatcctttt atacatataa ataatgtacc ttttgtaacc tttcttgaat catattgtac
     4321 attattaata tctatattta attcaccctt gacattgatt atatccaacc cctgtataat
     4381 atcatttaat ttcataaatg ctttcctccc tttaaactct ttctgctaaa attacactac
     4441 atataatatt atattaagaa ttttttacta tgtgaatggt taaaaatgct tgaaacattg
     4501 caaaaaatgc tctataattt tctataatat aactgaggaa aggtcttcta taaaaactat
     4561 ggataataaa atcgggtact taaacgataa ctttaaaata tttaatatta gagataaaaa
     4621 agatatacaa tttgaatacc ataatcatga ttttaataag ataattatat ttataaatgg
     4681 aaatgtaact tattttgttg atggtattcc ctataaacta aagccttggg atatactctt
     4741 tatcagcaat aatgaaatac ataaaccagt tatagattca aatgtctttt atgagagaat
     4801 tgcaatatgg attagcccag aatttatgaa aaaaaatagt gatgctacta gtgacttaga
     4861 aaaatgtttt aaaatagcta cagagaataa atgcaataga ttgcgtttaa atatacatga
     4921 tattgaatct attaagagtt ttattgagca aataaatatt tccgaatcca atactgaatt
     4981 tggaaatgaa atattgagaa atgcattatt tatacaactt atgatattct taaacagaat
     5041 tttcctaaaa aatgaaaata tggaatcaac agaagacgtt ttttatgatg aaactattaa
     5101 gaaagtatta aactatataa attcaaatat agatgataag ctttcaatag atagcatatc
     5161 gtctgaattt tttataagta agtattatct tatgagaaaa tttaaatctc atactggaac
     5221 gtctatacac aattatatag ttcaaaaaag attaattttg gcaaagtcac taataaccaa
     5281 tggtcacctt atgagtgaag tttgtaataa atgtggattc aacgattatt ctagttttgt
     5341 aagggcattt aaaaaaaact acggtgtatc tcctaaaaat tatataaaca ataaaaatcc
     5401 tttagatatt actcttttag atgaatagat aatttatgta aa
//
LOCUS       DAELLV010000225         5428 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.225, whole genome shotgun sequence.
ACCESSION   DAELLV010000225 DAELLV010000000
VERSION     DAELLV010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5428
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.225"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            150..530
                     /locus_tag="KPD58_003130"
     CDS             150..530
                     /locus_tag="KPD58_003130"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBG0266861.1"
                     /translation="MNIFDIGVDIIEIDRIRKAVDKNNRFLEKIFTDREIEYFNSKNF
                     KAESIAGNFAAKEAISKSIGTGIRLFNFKDIEVLRNEMGKPIVKTYNNLAKMCIDYNV
                     LEIKVSISHSKDYAIANAITIIKD"
     gene            545..1006
                     /locus_tag="KPD58_003131"
     CDS             545..1006
                     /locus_tag="KPD58_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBG0266862.1"
                     /translation="MMDKTAKEIMTTDVIVAKQDDSIADVANMLIAEKIGGLPVVDSE
                     NRVVGIISETDILKKEKYIEAPLYINLLQGLIFLDDLKKVEKDIKQVAAYKVGELMSK
                     DIIKVHEDDKFDDVANVMIKKSINRVPVVDDDNKLKGIICRYDIIKALYNE"
     gene            1027..1617
                     /locus_tag="KPD58_003132"
     CDS             1027..1617
                     /locus_tag="KPD58_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266863.1"
                     /translation="MRKKWTIVCIMFLALVIIVIGCQKRQSTKEEVYKDFQKQISDMN
                     YYSCKAEVEVVGNKSPHNYVLIHTYKKTDNYKLEVISPKHLKGKSIEYQGDKILVKNP
                     KISDVVELPNTGKNNQYLFVGDFIKNYLQNEEMKVKLSKGHLVLETFIPGDNKYFNKQ
                     VLYVNADTKNPEKMEVLDKEGVPRFTVKYKDFEYRN"
     gene            1632..2789
                     /gene="alr"
                     /locus_tag="KPD58_003133"
     CDS             1632..2789
                     /gene="alr"
                     /locus_tag="KPD58_003133"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBG0266864.1"
                     /translation="MQKITVPTWAEINLDNLRFNLNNIKNLLEEDIKICGVIKADAYG
                     HGAVEVAKLLEKEKVDYLAVARTAEGIELRQNGITLPILNLGYTPDEAFEDSIKNKIT
                     MTVYSLETAQKINEIAKSLGEKACVHVKIDSGMTRIGFQPNEESVQEIIELNKLEYID
                     LEGMFTHFATADEVSKEYTYKQANNYKFMSDKLDEAGVKIAIKHVSNSAAIMDCPDLR
                     LNMVRAGIILYGHYPSDDVFKDRLELRPAMKLKSKIGHIKQVEPGVGISYGLKYTTTG
                     KETIATVPIGYADGFTRIQKNPKVLIKGEVFDVVGRICMDQIMVRIDKDIDIKVGDEV
                     ILFGEGEVTAERIAKDLGTINYEVLCMISRRVDRVYMENNELVQINSYLLK"
     gene            2822..3109
                     /locus_tag="KPD58_003134"
     CDS             2822..3109
                     /locus_tag="KPD58_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HBG0266865.1"
                     /translation="MYDKSKEKIEVTLPDSLISEVDSIVQMENSNREDFAKAAFQFYI
                     TQKKKIHIKESMKTGYREMGQINLSLAELGMTVEEVSSDDKNEGKIAQGEY"
     gene            3078..3461
                     /locus_tag="KPD58_003135"
     CDS             3078..3461
                     /locus_tag="KPD58_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBG0266866.1"
                     /translation="MKGRLPKVSTNCEIKRGDLYYADLSPVVGSEQGGVRPVLIIQND
                     VGNKYSPTVIVSAITSQINKAKLPTHVEISSNEYGLNKDSVILLEQIRTIDKKRLREK
                     IGCLDENMMIKVDDGLQISLGLFTF"
     gene            3632..4459
                     /locus_tag="KPD58_003136"
     CDS             3632..4459
                     /locus_tag="KPD58_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBG0266867.1"
                     /translation="MRDHKGLNEIARIIRRDIVSMIHRAKSGHPGGSLSVVEILTALY
                     FDEMNVDSSNPKMEDRDRFVLSKGHAAPALYATLAEKGYFDKEELNGLRKIGRMLQGH
                     PDMKGTPGVEISTGSLGQGFSVACGMAMASKLDNAPWNVYTLLGDGEVQEGIVWEAAM
                     SAAHYKLDNLIAFLDNNGLQIDGDIESVMSLGSIVDKFKAFGWNVIEIDGHDFDQIFA
                     ALDIAKSTVKKPTMIVAKTIKGKGISFMENQAGWHGTAPSDEELEKALLELGGADNE"
     gene            4452..5393
                     /locus_tag="KPD58_003137"
     CDS             4452..5393
                     /locus_tag="KPD58_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HBG0266868.1"
                     /translation="MSKMATREAYGKALVKLGQINDNVVVLDADLSKSTKTHDFYKSF
                     PDRFFNMGIAEQNLIGAACGLSTAGKIPFASTFAMFATGRAFEIIRNSVCYPKLNVKI
                     CATHAGLTVGEDGASHESVEDIAIMRAIPNMTVLVPADGVETEKIIFEIAKYNGPVYV
                     RLGRSSVPVLFDEDYKFEIGKGTVLREGKDVSIIACGIMVNEALLAQEKLQEEGISAR
                     VINMSSIKPIDKDLILESAKETNAIVTVEEHSIIGGLGSAVSEVVGESCPTIVKKVGI
                     KDTFGESGTPNELLKKYELTCDDIIKTVKEVIIAKRM"
BASE COUNT         2167 a          569 c         1080 g         1612 t
ORIGIN      
        1 aaataatttt aaaagttaca gttttattat aatgattatt aaattaagat aagtaacata
       61 aactaaataa taatgtgtat tatcgatagt aaatttataa attcattatt aattattgaa
      121 agaagtatta taaattttct ggaggaacaa tgaatatatt tgatattggc gtagatataa
      181 ttgaaattga tagaataaga aaggctgtag acaaaaataa taggtttttg gagaagatat
      241 ttacagatag agaaatagag tattttaatt ctaagaattt taaagcagaa agtatagcag
      301 gtaatttcgc agccaaggaa gctataagta aatctatagg tacaggaatt agattgttta
      361 attttaaaga tatagaagtt ctcagaaatg aaatgggaaa accaatagta aaaacttata
      421 ataatctagc aaaaatgtgc atagattata atgttttaga aataaaagtt tcaatatcac
      481 attcaaaaga ttatgctata gcaaatgcga taaccataat taaggactaa ggagtgatta
      541 cattatgatg gataaaacag caaaagaaat aatgacaaca gatgtaatag tagcaaaaca
      601 agatgatagt atagcagatg tagctaatat gctaattgca gaaaaaatag gaggtctgcc
      661 agttgtagat tcagagaata gagttgttgg aataatttct gaaacagata ttcttaaaaa
      721 agaaaaatat atagaagcac ctctctatat aaatctatta caaggattga ttttcttaga
      781 tgacttaaaa aaagttgaaa aagatataaa acaagtagct gcttataagg ttggagaact
      841 tatgtctaaa gatataataa aggttcatga agatgataag tttgatgatg tagctaatgt
      901 aatgattaaa aaatcaataa acagagttcc tgtagtagat gatgacaaca aacttaaagg
      961 tataatttgt agatatgata taataaaagc tttatacaat gaataaactg aagtaggagg
     1021 tgtgatgtga gaaaaaagtg gaccatagta tgtattatgt tcttggcact ggttataatt
     1081 gtaataggat gccaaaaacg acagtccaca aaagaagaag tatacaaaga ttttcaaaag
     1141 cagatttctg atatgaatta ttacagttgt aaagctgaag tagaagtagt tggaaataaa
     1201 agtccacaca attatgtttt aattcatact tataaaaaaa ccgataacta taaattagaa
     1261 gtaatttccc caaaacattt aaaagggaaa agtatagaat atcaaggtga taaaatatta
     1321 gttaaaaatc caaagattag tgatgtagtg gagttaccta atacaggaaa aaataatcaa
     1381 tatctttttg taggagactt tataaaaaac tatcttcaaa atgaggagat gaaagtaaag
     1441 ttatccaaag gacatttggt tttagagact tttattcctg gggataataa atattttaac
     1501 aagcaagtat tatatgtaaa tgctgacaca aaaaatcctg aaaaaatgga agtgttagat
     1561 aaagagggag tgccaagatt tacagtaaaa tacaaagatt ttgaatacag aaactaaggg
     1621 ggactaaaga catgcaaaaa ataacagtgc ctacatgggc agagataaat ctagataact
     1681 taagatttaa cttaaataat attaaaaatt tattagaaga agatattaag atttgtggag
     1741 taataaaagc tgatgcatat ggacatggtg cagtagaagt tgcaaaattg ctagaaaaag
     1801 aaaaagtaga ttacttagca gtagcaagaa ctgctgaagg aattgaactt agacaaaatg
     1861 gcataacact tcctattttg aacttgggat atactccaga cgaagctttt gaagattcta
     1921 taaaaaataa aataactatg acagtttatt ctttagaaac agcacaaaag ataaatgaaa
     1981 ttgcaaaatc tttaggagaa aaagcctgtg ttcatgttaa aatagactca gggatgacta
     2041 gaataggttt ccaacctaat gaggagtcag tacaggaaat aatagaatta aataaattag
     2101 aatatataga tttagaaggt atgtttactc attttgctac agctgatgaa gtaagtaaag
     2161 agtacactta taaacaagct aataattata aatttatgtc tgataaatta gatgaggctg
     2221 gtgtaaaaat agctataaaa catgtatcaa acagtgcagc tattatggat tgccctgatt
     2281 taagattaaa tatggtaaga gcaggaataa tattatatgg tcattatcca tctgatgatg
     2341 tatttaaaga tagattagaa ttaagaccag ccatgaaatt aaaatcaaaa atcggacata
     2401 taaaacaagt tgaaccaggt gtaggaataa gttatggact aaaatacaca actacaggta
     2461 aagaaacaat agctacagtt ccaataggat acgcagatgg atttactaga atccaaaaaa
     2521 atccaaaggt tcttattaag ggagaagtgt ttgatgtagt tggtagaata tgtatggatc
     2581 aaataatggt tagaattgac aaagatatag acataaaagt tggagatgag gttatactat
     2641 ttggagaagg cgaagttaca gctgagcgta tagctaaaga cttaggaact ataaactatg
     2701 aagtgttatg tatgatatca agaagagttg accgtgttta tatggaaaat aatgagcttg
     2761 tacaaataaa cagttatttg ctaaaataaa accatgatat tataatctgg gaggcgattt
     2821 tgtgtacgat aagagtaaag aaaaaataga agttacttta cctgactctc tgatttctga
     2881 agttgatagt atagttcaga tggagaacag taacagagaa gattttgcaa aagctgcttt
     2941 tcagttctat ataactcaga aaaagaaaat tcatattaaa gaatctatga aaacgggtta
     3001 tagagaaatg ggtcaaatta atctgtcact agctgaatta ggtatgacag ttgaagaagt
     3061 atcttctgat gataaaaatg aagggaagat tgcccaaggt gagtactaat tgtgaaataa
     3121 agcgtggtga tttatattac gctgatttaa gtccagttgt tggttcagag cagggaggcg
     3181 ttagacctgt tctgataata caaaatgatg taggtaataa atatagtcct acagttatag
     3241 tatcagctat aacatctcag attaataaag ccaaattacc tactcatgta gagataagtt
     3301 ccaatgaata tgggcttaat aaagactcag taattctttt agagcaaatt agaactatcg
     3361 ataaaaagag actgagggaa aaaattggtt gtctagatga aaatatgatg ataaaagtag
     3421 atgatggact tcaaataagt ttaggattgt ttacttttta aagagggatt tgtcttttat
     3481 tagacaatcc cttttttctt ttaaatatct cttaaggttt aattttaatg tactatgtta
     3541 ataaaattaa aaataacgca aaaaaatagc ataaaagtga tataataata tggaattgat
     3601 ataaaattac gaactcaagg gaggtaattg catgagagac cataaaggat taaatgagat
     3661 tgcgcgtatc ataagaagag atatagtttc aatgatacat agagccaaat ctggacatcc
     3721 aggagggtca ttatcagtag ttgaaatact tactgcgtta tactttgatg aaatgaatgt
     3781 ggattcttca aatcctaaaa tggaggatag agatagattt gtattatcaa aaggacatgc
     3841 tgcaccagca ttatatgcta cattggctga aaaaggttat tttgataagg aagaactgaa
     3901 tggattaagg aagataggaa ggatgttaca aggtcatcca gacatgaaag gcacgccagg
     3961 tgtggaaata tctactggtt ctttaggtca aggtttttca gttgcatgtg gtatggctat
     4021 ggcatcaaag ttggataatg ctccgtggaa tgtgtatact ttgcttggtg atggtgaggt
     4081 tcaagaagga attgtttggg aagctgctat gagtgctgca cattataaac ttgataattt
     4141 aattgctttt ttagataata atggacttca aatagatgga gatatagaaa gcgtaatgag
     4201 tttaggttca atagttgata aatttaaagc ttttggttgg aatgtaattg agatagatgg
     4261 acatgatttt gaccaaatat ttgcggctct tgatatagct aaatcaacag ttaaaaaacc
     4321 tacaatgata gttgcaaaaa ctataaaagg caaaggaata tcttttatgg aaaatcaagc
     4381 aggatggcat gggacagctc caagtgatga ggagctagaa aaagccttgc ttgaattagg
     4441 aggtgctgac aatgagtaaa atggcgacga gagaagcata tggtaaagca ttagtaaaat
     4501 taggtcaaat aaatgacaat gttgtagtat tagatgcaga tttatcaaag tctacaaaaa
     4561 cacatgattt ttacaagtca tttccagata gattttttaa tatgggtata gctgaacaaa
     4621 atttaatagg cgctgcttgt ggattatcaa ctgctggtaa aatacctttt gcaagtactt
     4681 ttgctatgtt tgctacggga agggcttttg agattataag aaattcagtt tgttatccaa
     4741 agctaaatgt aaaaatatgt gctactcatg caggtcttac tgttggagag gatggagcat
     4801 ctcatgaaag tgtggaagat attgctataa tgagagctat accaaatatg acagttcttg
     4861 ttccagctga tggagttgaa acagaaaaaa ttatttttga aatagctaag tacaatggac
     4921 ctgtttatgt tagattaggg agaagttcag taccagtttt atttgatgaa gattataaat
     4981 ttgaaatagg aaaaggtaca gtcttaagag aaggaaagga tgtatcaatt atagcttgtg
     5041 gaattatggt aaatgaagct ctattagcac aagaaaagtt gcaagaagaa ggtataagtg
     5101 ctagagttat aaacatgtcc tcgataaaac ctatagacaa agatttaata ttagaatcag
     5161 caaaagaaac gaatgctatt gtaactgtag aagaacatag cataattggg ggattaggtt
     5221 ctgctgtaag tgaggtagta ggagagtctt gtccaaccat tgtaaagaaa gttggaatta
     5281 aagatacatt tggagaatct ggtactccaa atgaattgtt aaaaaaatat gaattaactt
     5341 gtgatgacat aataaagact gttaaagaag ttattattgc aaaaagaatg taagagatat
     5401 aaattattca tgtttgttaa taatataa
//
LOCUS       DAELLV010000226         5396 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.226, whole genome shotgun sequence.
ACCESSION   DAELLV010000226 DAELLV010000000
VERSION     DAELLV010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5396
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.226"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(944..2023)
                     /locus_tag="KPD58_003138"
     CDS             complement(944..2023)
                     /locus_tag="KPD58_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0266869.1"
                     /translation="MILITLCILVAVFVLAFAGLRLIAPTIIRTSHKIEAPGIDRMEM
                     VEIGGIQQALYIRGQNADNPIILWVHGGPGNSMMPFLHLYQYEWENDFTVVNWDQRNV
                     GKTYFANDPAAVLQTMSAERVLQDVHEVTAYLKQEFNKEKIILIGHSWGTVLGTMAVQ
                     TYPEDYSAYIGIAQTVNMVDNERVGYEKTLEMVKAAGKQKDIKALEAIAPYPPAKYGP
                     SYLAALMTVRTYQSKYGLSINMDLGNIISILATPYYSLRELKNTAIVNQNAIDNQGDV
                     LRFLVEEADIRNYGVDYQVPVYYIMGENDYNSPYLPAKKFFDEIVAPDKQIFFIPDAD
                     HLPFLSKKAEFDCVLLEEIAPKLAK"
     gene            complement(2107..2763)
                     /locus_tag="KPD58_003139"
     CDS             complement(2107..2763)
                     /locus_tag="KPD58_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0266870.1"
                     /translation="MTRKNNPKQAIENIITISAKLFAEKGYDKTSMQNIVDASGMSKG
                     GIFHHFSSKEDIFNVVMERRFEQIIETVNQWLGEMHGLTAKEKLRSLVRRHLTDEVIK
                     ESSNMITSAIESPHIILAFTQDNLKKLAPILANVLREGIEDRSIITEFPDECAEVILL
                     LFNFWCDTDVFQGDFPTLRKRFQFLQFLMRQIGVDILEDEIIESINIFYERKPMGQVE
                     "
     gene            3085..3291
                     /locus_tag="KPD58_003140"
     CDS             3085..3291
                     /locus_tag="KPD58_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266871.1"
                     /translation="MISHAAEICKKYKMPFIIFPTIEKKSIEEWVNIGANMLCLAQDT
                     TFITYVINQLLSFYNEIVPIQKQH"
     gene            complement(3444..3872)
                     /locus_tag="KPD58_003141"
     CDS             complement(3444..3872)
                     /locus_tag="KPD58_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBG0266872.1"
                     /translation="MSKIYIVYWSGTGNTEKMANFVAEGVKLKGKTPEVLDVSLLKPS
                     DLKEEDKFALGCPSMGAEQLEEGYMEPFVSELESMVSGKQIGLFGSYGWGNCEWMRDW
                     EERMQNAGATIIGGEGITAMEDPNEEAKDECIELGKTLAE"
     gene            complement(4348..5286)
                     /locus_tag="KPD58_003142"
     CDS             complement(4348..5286)
                     /locus_tag="KPD58_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HBG0266873.1"
                     /translation="MNKRMKLIPYEINENLRGAKNKFPYGIKQMNARGMWDEGYTGKN
                     IVVGIIDTGCDISHPLLKGKIIGGANFSDDSNGNKNIYEDFNGHGTHVAGIIAASNYN
                     NEVMGVAPDCKLLIAKALNKDGTGTYQSIINAINFAVNNKVDIISMSLGGNKDDKNLK
                     NAVMQAVKNNISVVCAAGNNGDGDSSTSEYSYPASYAEVIEVGAINENYLVEKFSNSN
                     TTIDLVAPGRNIISTYMDNKLAIMSGTSMSAPYVSGSLALIKEWAREEFERDLDEAEL
                     YAQLIKCTRALGIPRTEQGNGYLYLNLYKYKNNGKR"
BASE COUNT         1661 a         1063 c          672 g         2000 t
ORIGIN      
        1 agaaatacat ctaagaaaga atattattaa attgttccaa ttagtattat ttttcttata
       61 aaaataaaaa aactgttgga ttcttttaga aaaattattt tttacatacc ttttttgctt
      121 agatagtcta tcttctcagc tattttgtat atacactctt ctagttcatg attttatttt
      181 aatatcttgc ttatattgct gaaataattg aattctttta tctatcatat tgtctcttat
      241 atcataaaca tattgtttct tatatcacaa aaatgaaagc attcttatat cttcaatata
      301 taaacctgct ttttttaatc ttgtaataaa tataggatac tctatattct cttcctagaa
      361 ataactaata ccacttattt ttctattata tagaaagtaa taaatctttt tcttaataaa
      421 attcaatagt aagtggaata aaattttata tgaataagaa cattataaaa atactttata
      481 caatagtcaa taaaagatat tagcttcaaa ttatgttaaa gttgtattat ttaaagtgaa
      541 aaaacaaata aaaaagaaat aatagtataa gtagaaaaat atacttaaaa caaggcgaag
      601 cctctcttgt atctaaatca agtagagaat taaatctaaa aaagaaaata aatataaaag
      661 atgactcctg tacattacaa gaatcatctt atggtttcat aatcactcca tggtttttaa
      721 ctgtctgata taaaggtctc agttcactca tagcatgacc accctttccg attgctgacc
      781 ccgtcactta tggacaaaat gtccataagt gatagtcctc tcttaaaacc ctctaaatag
      841 atatatctca agcacctcat tgaaaccagt tgctggaaac aaactgaagc gttctggaat
      901 agtaaaagaa attgccacca tatccacgtt actgtcctag ttgttacttt gctaacttcg
      961 gagcaatttc ctctaacaat acgcagtcaa attctgcctt tttgctaaga aatggcagat
     1021 ggtctgcatc tggtataaaa aaaatctgtt tgtctggcgc cactatttca tcaaaaaatt
     1081 tcttcgctgg aagatagggg ctattatagt cattttctcc catgatgtag tatacaggga
     1141 cctgatagtc aactccataa tttcttatgt cagcttcttc cactaaaaac cgcaatacat
     1201 caccttgatt gtcaatcgca ttttgattga caatggccgt attttttaac tctctcaatg
     1261 aataatacgg ggttgccagt atactgatta tattgcccaa atccatattt atagaaagcc
     1321 catacttgct ttggtatgtt cttactgtca taagagctgc caaatagctt ggaccatatt
     1381 ttgctggcgg atatggtgca atagcttcca gcgccttgat atctttttgc ttccctgcag
     1441 ctttcaccat ttcaagtgtc ttttcatagc ccacgcgctc attgtccacc atattaacgg
     1501 tctgcgcaat cccaatgtac gcgctgtaat cctccggata ggtttgcaca gccatagttc
     1561 ccagcactgt tccccagcta tgacctataa ggataatttt ctccttgttg aactcttgct
     1621 tcaaatatgc tgtcacctca tggacgtctt gcagaacacg ctccgcgctc atggtctgca
     1681 aaactgctgc cggatcgttc gcaaagtatg ttttgcctac gtttcgctga tcccagttca
     1741 ccacagtaaa atcattttcc cactcatact ggtacagatg taaaaatggc atcattgaat
     1801 taccaggacc cccatgaacc caaaggatga tggggttgtc tgcgttttgt ccccggatat
     1861 ataacgcctg ctgtatgccg ccgatctcca ccatttccat gcggtcgatt ccaggagctt
     1921 ctattttatg gcttgtacgt ataatcgtgg gggctatcag acgcaaaccg gcaaacgcca
     1981 gaacaaaaac agcaactagt atgcaaagtg tgattaatat cattttaagt accttctttc
     2041 atgaattttt ttagccatag atatctcctc taacattaaa aaaaatatgt tgctatttta
     2101 gttttgttac tctacctgtc ccattggttt cctttcataa aaaatattaa ttgattctat
     2161 aatctcatct tccaggatat caactcctat ttgtctcata agaaactgta agaattgaaa
     2221 ccgtttacga agtgtaggaa agtccccctg aaatacatct gtatcacacc aaaagttaaa
     2281 gagcagcaaa attacctctg cgcattcatc tgggaactct gtgatgatgc ttctatcctc
     2341 tatgccttct cgtaaaacat ttgctaatat tggtgctagt tttttcaaat tatcctgtgt
     2401 aaatgccagt atgatatgcg gactttctat agcgcttgta atcatgttac tagattcttt
     2461 tatcacctca tccgttaaat gccgcctaac taaacttcta agcttctctt ttgctgtaag
     2521 tccatgcatt tcaccgagcc attgatttac tgtctctatt atctgttcaa agcgcctttc
     2581 catgaccaca ttaaaaatat cttcttttga actgaagtga tgaaaaattc cgccctttga
     2641 cattcccgaa gcatcaacaa tattttgcat acttgttttg tcatatcctt tttcagcaaa
     2701 caattttgca gaaatggtaa tgatattttc aatagcttgc tttggattgt tctttctagt
     2761 cattatataa ctccttctta ttaaaaaccg actgttggtt tttattatat aagctattcc
     2821 tacttctgtc aagcaacatt atgtttatgg caaaacaata aggtttggtg gttagacagg
     2881 tattaattag actaatgcga ctttccccta gatggaaccc ttggtaaatt tgcaatcttt
     2941 accggctttg attatatctg taagcaaggc aggtatggcc acacctgccc tgtttgaccc
     3001 tcggatattc attcctatcc cttctctcct tagccattac accaaaacgc cgctcattgt
     3061 ctccaatggc ggcgttttaa agatatgatt tctcatgctg ctgaaatatg caaaaaatat
     3121 aagatgccct ttattatttt tcctacaata gaaaagaaaa gtattgaaga atgggttaat
     3181 attggagcaa atatgctttg tttggcacaa gatactacct ttatcactta tgttatcaat
     3241 caattattat ctttttataa tgagattgta ccaattcaaa aacaacatta aattataaat
     3301 ctaattttat attaaaagag actaatattt tatctatctc tgttaaggat attttcttat
     3361 gtatcataat attagtctct cttattatta tatactgcta tcatcattag ttgctattta
     3421 tactattttt ataatataca aatttattca gctaacgttt tgcctaattc tatacactca
     3481 tcttttgctt cttcatttgg gtcttccatt gctgtaattc cttctccacc aataattgta
     3541 gcaccagcat tttgcatacg ttcttcccag tctctcatcc attcacaatt tccccatcca
     3601 tatgaaccaa ataatccaat ctgtttacct gataccatag attctaattc tgaaacaaat
     3661 ggctccatat acccctcttc tagttgctct gctcccatag atgggcaacc taatgcaaat
     3721 ttatcttctt cttttaaatc acttggtttc agtaagctca catctaaaac ttctggtgtc
     3781 ttacctttta acttcacacc ttcagccaca aaatttgcca ttttttcagt atttcctgtt
     3841 ccactccaat aaacaatata tattttactc atttttacct cctgtagtat tttttaatta
     3901 atcaatagtt agttatagct aactaacatc taaaaaacaa aagatttctt tatctgaaat
     3961 ctctttattt ttctaaatat agaatatcat agttgatttt gaaattcaat gcttattgat
     4021 aatatttatc atttatattt tatattcatt ttataatatc aaaacactgc atcgtatttc
     4081 taatctttca tagaaaaatt tctaacttat agtacttttg catctcttat aaatctttta
     4141 tatgtataag tacatagaaa aacacacctt ttccagatgt gctatctata atatcgtata
     4201 tttatttttc aattttaact agctaatatg tttataaatt ttatcactta atacctttat
     4261 acattgatgt aaaatcttac acctaagtaa tttttattat ttgtctcgac tctatataat
     4321 atagctattt tatcatcaaa tcaaaaatta tcttttgcca ttattcttgt atttatagag
     4381 atttaaatat aagtatccat ttccttgttc cgttctaggt attccaagcg ctctcgtaca
     4441 ttttattaat tgtgcataca gttcagcttc atctaaatct ctttcaaact cctctcttgc
     4501 ccattcttta attagtgcta atgagcctga tacgtatggt gcactcatac tggtaccact
     4561 cataatagca agtttattat ccatataagt cgatataata tttcttcctg gagctaccaa
     4621 atctattgta gtatttgaat tactaaactt ttcaaccaaa tagttttcat ttattgcacc
     4681 tacttctatt acctcagcat aactggctgg ataactatac tcacttgtac tagaatcacc
     4741 atctccatta ttacctgctg cacacactac agaaatatta ttttttactg cttgcatgac
     4801 agcatttttt aaattcttat catctttgtt tccaccaaga gacatagata taatatcaac
     4861 cttgttattt acagcaaagt taatagcatt aattatactt tgatatgttc cagtaccatc
     4921 tttatttaat gcttttgcta ttaataattt acaatctgga gctactccca taacttcatt
     4981 attatagtta gatgcagcta taatacccgc cacatgagtt ccatgaccat taaaatcctc
     5041 atatatattt ttatttccat tactgtcatc actaaaattt gcaccaccaa ttatttttcc
     5101 ttttaaaaga ggatgagata tatcacaacc tgtatctatt ataccaacta caatattttt
     5161 accagtataa ccttcatccc acattcccct agcattcatt tgttttattc catatgggaa
     5221 tttatttttt gcacctctta gattttcatt tatttcatac ggaattagtt tcattctttt
     5281 attcatcata tatcctccta aaaattttat tgcttataaa aatcatgaat tttattttat
     5341 aataggttta taaatcaaat taaaacctat atctacttat ttatatttat gatata
//
LOCUS       DAELLV010000227         5386 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.227, whole genome shotgun sequence.
ACCESSION   DAELLV010000227 DAELLV010000000
VERSION     DAELLV010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5386
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.227"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(204..1388)
                     /locus_tag="KPD58_003143"
     CDS             complement(204..1388)
                     /locus_tag="KPD58_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG0266874.1"
                     /translation="MLSKRLNFITPSYTIGISSKVKEMESNGVKVINLSIGEPDFNVP
                     NNAKSYGIDSLNKDYTKYDLVPGLKILREEICKKLIEENNCNYSIDEIVVSSGAKNSI
                     TNTLLALTDEGDEVLLPKPYWVSYPEMVKLVNAVPVFIDTKKENGFKLTKDELEKSIT
                     DKTKILVINNPSNPTGSVYTKDELIEIVDVCIQNKIYILADEIYEKICYTGEFTSIAS
                     LSEEAKDITITINGFSKSAAMTGLRLGYTASNKTIAKAMSSIQGHLISHPSLTAQYIA
                     YGALKDCSIDIDNMVKTYKSRRDLIKSKLDSIDNVGYVNPNGAFYIFIDLSKVSEKFE
                     YKDSFSIEFCNQFLEEYNVAVVPGIAFGMDKYIRISYACSENTFLSGLDKLKEFVYKI
                     MA"
     gene            1761..4112
                     /locus_tag="KPD58_003144"
     CDS             1761..4112
                     /locus_tag="KPD58_003144"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HBG0266875.1"
                     /translation="MVEFVKKRDGRVIPFNEDRITRAIFLAATNVAEREGIVPDYKLS
                     EQLTQEVIKFLNHKYSESVPSVEDIQDSVVKVLIETGHAKTSEEYIIYRTERSRIRNS
                     KTRLMKAIEEITFEDAEDADIKRENANINGNTAMGTMLQYGSTVSKEFCKTHILKPEH
                     SFAHDNGDIHIHDMDFLNMGTLTCCQIDVKKLFNGGFSTGHGFLREPQDIISYGALAA
                     IAIQSNQNDQHGGQSIPFFDYGLAEGVYKTFKKFYIGNLAKALKLFKGIENNDVIKNI
                     VYNTEKETNQKVGLKRDELYLNLEKEKLIQTFDIDDELVNKMQNFAFEESYRETDKKT
                     YQSMEAFIHNLNTMHSRAGAQVPFSSVNFGTDTSEEGRMVTKNLLLSQERGLGNGETP
                     IFPILIFKVKEGINLNPEDPNYDLFKLSCRVSAKRLFPNFSFLDAPFNAKYYKKGEPD
                     TEATYMGCRTRVLSNVCGSETVSGRGNISFTTVNLPRLGIKHGIINNEKANLDGFFEE
                     LDEKINLIIEQLLERLEVQGNKKMKNFPFLMGQGVWKGSDDLGPEDTLKEVIKQGTLT
                     IGFIGLAECLIALIGKHHGESKEAQELGLKIVSHMRHKMDEATDKYKLNFSLMGTPAE
                     GLSGRFTKIDKKVYGEIKGITDKEYYTNSFHVPVYYNISAYDKIEIEAPYHELTNAGH
                     ITYVELDGDPSDNLEAFETVIKAMKDLGIGYGSINHPVDRDPICGFSGVITSNICPVC
                     GRNEDESDIKFERIRRITGYLVGTVDRFNNAKKAEVRDRVKHR"
     gene            4134..4673
                     /gene="nrdG"
                     /locus_tag="KPD58_003145"
     CDS             4134..4673
                     /gene="nrdG"
                     /locus_tag="KPD58_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HBG0266876.1"
                     /translation="MKIRMSSTISYDSIVDGPGLRMVIWTQGCIHNCKECHNPQTHDL
                     CGGFYMDTEEIINKVKSLKLQKGITLSGGEPFLQPEPLEEIAREAKINGLDVWSYTGF
                     TFEQLLDKKNRAYFKNLNLLKQIDILVDGKFIAEKKDISLKFRGSSNQRIIDVQKSLK
                     YKKVFLVEQYMKDDLSIAE"
BASE COUNT         2112 a          600 c          856 g         1818 t
ORIGIN      
        1 ttataataaa tttagaattt aacttatata gtttaatttg ataatttgat gaaaaaaata
       61 aaagacttac tgatttttaa atctataggt cttttatttt ttatctaaca tttaaattaa
      121 gttgttgaat atatcttata ttacttacct tatatattaa ttaccattaa tacattatat
      181 ctaattacta ttcatctaaa ttattatgcc attattttat atacaaattc ttttaattta
      241 tctaatccag ataagaaagt attttcactg caagcatagg atatacgaat atatttatcc
      301 ataccaaatg ctatgccagg aacaactgca acattatatt cttctaaaaa ttgattacaa
      361 aattctatag aaaaactatc tttatattca aatttctcag atactttaga aagatctata
      421 aatatataga atgcgccatt tggattcaca tatcctacat tatcaataga gtctagtttt
      481 gatttaatta aatctcttct tgatttatat gttttaacca tattgtctat gtcaattgag
      541 caatctttca aggctccata ggctatgtat tgtgctgtta gacttgggtg agatataaga
      601 tgtccttgta tagaactcat tgctttagca atcgttttat tagatgcagt atatcctaat
      661 cttaatccag tcattgcagc tgacttagaa aaaccattta tagttattgt tatatcttta
      721 gcttcttcgc ttaatgatgc aattgatgta aattcaccag tgtagcatat tttttcatat
      781 atttcatctg ctaaaatata tattttattt tgtatgcata catctactat ttctattaat
      841 tcatcttttg tataaacact accagtagga tttgaaggat tgtttattac aagaattttt
      901 gttttatctg ttatagattt ttcaagttca tcttttgtta acttaaatcc attttctttt
      961 ttagtatcaa taaaaactgg aacagcatta actaacttaa ccatttcagg ataactaacc
     1021 caataaggct ttggtaataa gacttcatct ccttcgtctg ttaaagctag taaagtattt
     1081 gttattgagt tttttgctcc acttgataca actatttcat ctattgaata gttacagtta
     1141 ttttcttcaa taagtttttt acagatttcc tctctaagta tttttaatcc tggaactaaa
     1201 tcatatttag tgtaatcttt atttaaagaa tcaataccat aagattttgc gttattaggt
     1261 acattaaaat cgggttctcc tatactaaga tttattactt taactccatt actttccatt
     1321 tctttaactt tagaactaat acctattgtg taggatggtg ttataaagtt tagtcttttt
     1381 gataacataa aatgcctcct tttaaattat tatttatatt atacgacaaa tttaaagaat
     1441 ttacataagg aaatttgtag atatatacat taatatatat aatagcctac actttttgcc
     1501 taaaattagt gtaaaaaaaa gagaaaaaaa ttttaacttt caaagttgtt agaaacgcta
     1561 ctgttcaaaa tatccacata gtatttgcaa tgatttgcgc aagtttgact attatgtaca
     1621 aatttggtaa tcttaatata gcaattatta agataatccc tttaacttag ttgaagggga
     1681 tttttttaga cttaacatac tacatatagt ggtatgggga ttaataaaat acaatatata
     1741 gtaacaggag gtttttttaa ttggttgaat tcgtaaaaaa aagagatgga agagtaattc
     1801 catttaatga agatagaata acaagagcta tatttttagc agcaacgaat gttgcagaaa
     1861 gagaaggaat agtacctgat tataaattat ctgagcaatt aactcaagaa gtaataaagt
     1921 ttttaaatca taaatactca gaatcagttc caagtgttga agatatacaa gattctgtag
     1981 ttaaggttct tatagaaact ggccatgcaa aaactagtga agaatatata atatacagaa
     2041 ctgaaagaag tagaataaga aattcaaaaa caagattgat gaaggcaata gaggaaatta
     2101 cctttgaaga tgcagaagat gctgatataa aaagagaaaa tgcaaatatt aatggtaata
     2161 cagcaatggg aactatgtta caatatggaa gcactgtatc taaagaattt tgtaaaacac
     2221 atatattaaa accagagcat tcttttgctc atgataatgg agatatacac atacatgata
     2281 tggatttttt aaatatggga acattaactt gttgtcaaat agatgttaaa aaactattca
     2341 atggtggatt ttcaacagga catggctttt taagagagcc acaagatata ataagttatg
     2401 gtgcattagc tgcaattgct atacaaagca atcaaaacga tcaacatgga ggtcaaagta
     2461 taccattttt cgattatgga ttggctgaag gtgtatataa gacatttaag aaattctaca
     2521 taggaaattt agctaaagca ttgaagttat ttaaaggaat tgaaaataat gatgtgataa
     2581 agaacatagt atataataca gagaaagaaa caaatcaaaa agttggtctt aagagagatg
     2641 aattgtatct aaacttagaa aaagagaaat taatacagac ttttgatata gatgatgaac
     2701 ttgtaaataa gatgcaaaac tttgcatttg aagaatcgta tagagaaact gataaaaaaa
     2761 catatcaatc tatggaagca tttatacaca atctaaatac aatgcactct agagcaggtg
     2821 cacaggtacc attttcaagt gtaaacttcg gaacagatac ttctgaagaa ggaagaatgg
     2881 taactaagaa tcttctatta tctcaagaaa gaggtttagg taatggagag acaccaattt
     2941 ttccaatttt aatatttaag gtcaaagaag gcataaattt aaatcctgaa gaccctaatt
     3001 atgatttatt taaattatca tgcagagtat cagctaaaag actattccct aattttagct
     3061 ttttagatgc gccttttaat gcaaaatatt ataaaaaagg tgaaccagat acagaggcta
     3121 catatatggg ttgtagaaca agagtactta gtaatgtgtg tggttcagaa acagttagtg
     3181 gtagaggaaa catatctttt actacagtaa atttaccaag attgggtata aaacatggta
     3241 ttataaataa tgaaaaggct aatttagatg gtttctttga agaattagat gagaagataa
     3301 acttaattat agaacaatta ttggagcgcc ttgaggtaca aggaaataaa aagatgaaga
     3361 attttccatt cttaatgggg caaggtgtat ggaaaggttc tgatgattta ggaccagaag
     3421 atacactaaa agaagtaata aaacaaggaa cattgacaat tggatttata ggtttagcag
     3481 agtgccttat tgctcttata ggtaaacatc atggagaaag taaagaagct caagaattag
     3541 gattaaaaat agtgtcacat atgagacata aaatggatga agcaacggac aaatataaat
     3601 taaatttctc cttaatgggt actccagcag aaggtctttc aggtaggttt actaaaatag
     3661 ataaaaaagt ttatggagaa attaaaggta tcacagataa agaatattat actaattcat
     3721 tccatgtacc agtttattac aatataagtg catatgataa aattgagata gaagctccat
     3781 accatgaatt gactaatgca ggtcatataa catatgtaga gttagatgga gatccatctg
     3841 ataatttaga agcttttgaa actgttataa aagctatgaa agatttgggt ataggatatg
     3901 gaagtataaa tcatcctgta gatagagacc caatatgcgg tttttcaggt gtaataacaa
     3961 gtaatatatg ccctgtatgt ggaagaaatg aagatgaaag tgacatcaaa ttcgaaagaa
     4021 taagaagaat aacaggatac ttagttggta cagttgatag atttaataat gctaaaaaag
     4081 cagaagtaag agatagagta aaacatagat agttttattt gggggaataa gttatgaaaa
     4141 taagaatgtc atctactata agttatgaca gcatagttga tggaccagga ttaagaatgg
     4201 taatatggac tcaaggatgt attcataatt gtaaagagtg ccataatcct cagacacatg
     4261 atttatgtgg aggattttat atggatacag aagaaattat aaataaagtc aaatcattga
     4321 aattacaaaa aggcattacg ctatcaggag gagaaccatt tttacaacca gaaccattag
     4381 aagaaatagc aagagaagct aaaataaatg ggttagatgt atggtcatat actggattta
     4441 cgtttgaaca actgttagat aaaaaaaata gagcgtattt taaaaattta aatttactaa
     4501 agcaaattga tattttagtg gatggaaaat ttatagctga aaaaaaagat ataagcttaa
     4561 agtttagagg gtcatcaaat caaaggataa ttgatgttca aaaaagtttg aaatataaaa
     4621 aagtgttttt ggttgagcaa tatatgaagg atgatttatc tatagcagaa taaaatataa
     4681 ataaacagga ttaaatatat gcatatattt aatcctgttt attttcatat aatatttcaa
     4741 gcttgtataa tgtaacaatt aaaccaaaag actcattgta tatttgaaat taagtttttt
     4801 atagtttata ggtgttttat gtggattacg aatgatatag tattgataca ttatttaata
     4861 gattcttatg ccaagaaaca attaatttat agaaattctt aattgttaca atataaatat
     4921 ctagataagt tataagatac tgataaataa tttataaagt ttattaatct aatattgtgt
     4981 gtgaatatta agtggtcaag tagtctaaaa aatgaaaaag atttaaaaaa gtgttttaac
     5041 taaacacata agtgttaaat tcatgtataa gtataggtta gatacataag aaataattat
     5101 gtaatatcta tctttgtaaa aaaatattaa attttgtaat ttgtggataa atatgtttat
     5161 aatgtggata atatttaatg taataagtta aagcaatatg aatcaactgt ttataatgtg
     5221 gataatatta taaatgtatg aataattgca gtatttaaaa acaaaaaaag ttaaaaaagt
     5281 tgttgactct aaaagaaaaa gttggtatag taatacttgt cctcaagaaa agggcaaaca
     5341 aataaagcat aaagaacttt gaaaattaaa cagtaggtta atttat
//
LOCUS       DAELLV010000228         5320 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.228, whole genome shotgun sequence.
ACCESSION   DAELLV010000228 DAELLV010000000
VERSION     DAELLV010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5320
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.228"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(74..2293)
                     /locus_tag="KPD58_003146"
     CDS             complement(74..2293)
                     /locus_tag="KPD58_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG0266877.1"
                     /translation="MTGRNKSFTLITILFLTSLFLTMGYIYIEDTKHLLMSEAKIHIK
                     EVAVQGSQLAQRQIEKDLDVLNIFSNYYASNPDIPNEEKMKNLLDEMENQKFYTMAIV
                     DINGNAENTKGDKFSVKDREFFKNSIKGKKYVSSPYVDEVNKSIKKIAISVPLLNNDK
                     VVGVLYCTYNINTLMKLINISFYENNSISYVVKNNGTIILHPQGDSLSKNIYKLLKQD
                     NDIQEVNRLKKELQENKTGATVLNMLEERRYLGYATMDNGNSENNYIKDWNLIFSIPE
                     TVVFSNSKQIINRAVYAVLSIVLIFIAIIFYIIYIKKSNEKEILSLAYEDKVTYIGNQ
                     NKFYRECSKYLLDKPSLNYIIVYFDINNFKMINDTFGYEFGDNLLITIAKALKEELTE
                     GEVYARLSSDYFAIFCDYKNGRNKIIRKLDNIRSNIESNLSIVFEISLCVGIYFVEEG
                     EVDIQKAVNKANMARSVAKGKNINYAIYNEDVRNKLSEESMILDDIKIALVKNQFEVY
                     YQPKFSLVTGEMIGSEALIRWNHPEHGFISPAVFIPIAEKSKLILKIGRFVFERVCND
                     LYEWKKQGKKIVPVSVNLSRVELYQPDIVKFINKTIKMYNLSSDFIEIEITETVAINE
                     LNILKNVLNELRTYGFSISMDDFGTGYSSISCLRDMPIDILKLDKSFLGGIEHDERSR
                     NIAKSIVSLAKSLDLVVIIEGVESKEQAELMKQFGCDLVQGFYFARPMPAKNFLDLL"
     gene            complement(2569..5223)
                     /gene="adhE"
                     /locus_tag="KPD58_003147"
                     /gene_synonym="adhC"
     CDS             complement(2569..5223)
                     /gene="adhE"
                     /locus_tag="KPD58_003147"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBG0266878.1"
                     /translation="MAHKEQVIIENKKENEVMSVGLDTVNNVESLVKKLAKIREAQKI
                     FATYSQEQVDKIFLAASLAANKQRVPLAIMAQKETGMGIAEDKVIKNHYASEYIYNAY
                     KETKTCGVIEKDEAFGMTKIAEPIGVIAAVVPTTNPTSTAIFKALLALKTRNGIIFSP
                     HPRAKNSTIEAAKVVLEAAVLAGAPEGIIGWIDEPSLELTTTVMKEVDLTLATGGPGM
                     VKSAYSSGKPAIGVGAGNTPAIIDDSADIKTAVNSILVSKTFDNGMICASEQSVIVLE
                     NIYNEVKKEFKERGAYLLDKDETEKIRNIILVNGGLNSKIVGQTACTIAKLAGFEVPV
                     DTKVLIGEVESVEIEEAFAHEKLSPVLAMYKASDFDDAVRKAEKLVEDGGFGHTSSLY
                     IDDVNQREKLDKFTSAMKTCRILINTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVS
                     ENVGVKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVALKELKDVMNKKRAFIVTD
                     TFLYNNGYTKAVTDLLDEMNIKYTTFFEVEPDPTLECAKIGAKAMREFNPDVIISIGG
                     GSAMDAGKIMWTLYEHPDVDFQDLAMRFMDIRKRVYTFPKMGEKANFVAIPTSAGTGS
                     EVTPFAVITDQDTGVKYPLADYELMPNMAIVDSDMMMNMPKSLTSASGIDALTHALEA
                     YVSMLATDYTNGLALQAIKSIFEYLPRAYDNGAKDPEAREKMANASTMAGMAFANAFL
                     GVCHSMAHKLGAFHHVPHGVANALLITEVMKFNSSDAPKKMGAFSQYKYPEALKRYAE
                     IASFLGLKGNSDEEKFQSLLVAIEDLKLKVGIPKSIKEFGVEESKFMDSIDEMVIQAF
                     DDQCTGANPRYPLMNEIKDMYLNSYYGR"
BASE COUNT         1721 a          959 c          572 g         2068 t
ORIGIN      
        1 actaattatt tagcaatgat ttattaaaat caagcattca taatagtgct ttatataaaa
       61 atcaaatttt gttttaaagt aaatctaaaa aattcttagc tggcattggt cgtgcaaaat
      121 aaaatccttg aactaaatca catccaaatt gtttcattaa ctcagcttgt tctttactct
      181 caacaccttc tataattaca actaaatcta gtgattttgc tagagacact attgattttg
      241 ctatattgcg acttctctca tcatgttcaa tgccaccaag aaaagactta tctagcttca
      301 atatatcaat tggcatatct cttaaacaac taatagaaga atatcctgta ccaaagtcat
      361 ccatagaaat agaaaatcca tacgtcctca actcatttaa aacattcttt aaaatattta
      421 actcattaat agccacagtt tctgttatct ctatttcaat gaaatctgaa cttaaattat
      481 acatttttat tgttttattg ataaatttta caatatctgg ttgatacaac tcgaccctag
      541 ataaatttac tgaaactgga actatctttt taccttgctt tttccattca tataaatcat
      601 tgcaaaccct ttcaaataca aatctcccaa tttttaaaat caacttactt ttctctgcta
      661 ttggaataaa tactgcggga cttataaaac catgttctgg atgattccat cttattagtg
      721 cttcactccc tatcatttct ccagttacaa gtgaaaactt aggttgataa tacacttcaa
      781 attgattttt taccaatgct atttttatat catctaaaat cattgattcc tcactaagct
      841 tattcctaac atcttcatta taaatagcat aatttatatt tttaccttta gcaacagacc
      901 tagccatatt tgctttattt acagcctttt gaatatctac ttcaccttct tcaacaaaat
      961 aaatacccac acacagagaa atttcaaaga ctatactcaa attactttca atattacttc
     1021 gtatattatc aagttttctt ataattttat ttcttccatt tttataatca cagaaaattg
     1081 caaaataatc acttgaaagt cgtgcatata cctcaccttc tgttaattct tcttttaaag
     1141 cttttgctat tgtaattaat aaattgtcac caaattcata tccaaaagta tcattaatca
     1201 tcttaaagtt attaatatca aaatatacta taatataatt taaagatggt ttatctaaaa
     1261 gatacttact acattctcta taaaatttat tttgatttcc tatatatgta actttatctt
     1321 cataagccaa acttaatatt tctttttcat tagatttttt tatataaatt atataaaaaa
     1381 taattgcaat aaatattaat acaatagata acactgcata tacagctctg ttgattattt
     1441 gttttgaatt actaaataca acagtttctg gaattgaaaa aattaaattc cagtctttta
     1501 tataattatt ttcactattc ccattatcca tagtggcata tcctaaatat cttctttctt
     1561 ccaacatatt taatacagtt gcacctgttt tattttcttg caattctttt tttaatctat
     1621 ttacttcttg aatatcatta tcctgtttta acaatttata aatatttttt gataggctat
     1681 ccccttgtgg atgaaggata attgtaccat tattttttac aacataagat atactattat
     1741 tttcataaaa agatatatta ataagtttca tcagagtatt gatattgtaa gtacaatata
     1801 aaactccaac tactttatca ttatttaata atggtactga aatagcaatc ttctttatag
     1861 acttatttac ttcatcaaca tatggacttg aaacatactt cttaccttta attgagtttt
     1921 tgaaaaattc tctatctttc actgaaaact tatcaccttt ggtattttca gcattaccat
     1981 ttatatcaac tattgccata gtgtaaaatt tttgattttc catttcatct aaaagatttt
     2041 tcattttttc ctcattaggt atatctggat ttgaagcata atagttagaa aaaatattta
     2101 aaacatctaa atctttttca atttgtcttt gtgctagttg agagccttgt actgcaactt
     2161 cttttatatg tatttttgcc tctgacataa gaagatgttt tgtatcttct atatagatat
     2221 accccattgt caaaaataat gatgttaaaa aaagaattgt aattaatgta aaacttttat
     2281 ttctccctgt cattcgactc ctcccctaat tagtcaatac ttcatataga taatatatcc
     2341 tttttatacc aattcatcat ttatattgta tataactatt cttaaatgtt acacataaaa
     2401 tcaacataat aaccataaaa acattctttt attattataa taacactatt taattgaatt
     2461 ttaaattaaa gaataaaaac tatccatttt ctcaaacaaa aaaactctta tacaataaaa
     2521 ctttgtttta tgtataagag tttttatttt atctatttta tagcaaactt atcttccata
     2581 atatgaattt aaatacatat cttttatttc attcataagt ggatatcttg gatttgctcc
     2641 tgtacattgg tcatcaaatg cttgaattac catttcatct attgaatcca taaacttaga
     2701 ttcttcaacg ccaaactctt ttatagattt aggaatacca actttaagtt ttaaatcttc
     2761 aatagctacc aataaacttt ggaatttctc ttcatcagaa tttcctttta atcctaaaaa
     2821 tgaagctatt tcagcatatc tctttaatgc ttctggatat ttatattgag agaatgctcc
     2881 cattttcttt ggtgcatctg aagaattaaa tttcataact tctgttatca aaagagcatt
     2941 tgcaacacca tgtggtacat ggtggaaagc acctaattta tgagccatag agtgacaaac
     3001 acccaaaaat gcatttgcaa aagccatacc tgccattgta gaagcatttg ccattttttc
     3061 tctagcttct gggtcttttg caccattatc atatgcacgt ggaagatatt caaatatact
     3121 cttaatagct tgtaaagcta atccatttgt ataatcggtt gcaagcatag atacatatgc
     3181 ttctaatgca tgagttaaag catctatacc agaagcagat gttaagcttt ttggcatatt
     3241 catcatcata tcagaatcaa ctatagccat atttggcatc agttcataat ctgctaatgg
     3301 atattttact cctgtatctt ggtcagttat aacagcaaat ggtgttactt ctgaaccagt
     3361 acctgctgat gttggtatag ctacaaaatt agctttttca cccattttag ggaatgtata
     3421 tacccttttt cttatatcca taaatctcat agctaggtct tggaaatcta catctggatg
     3481 ctcatacaat gtccacatta tctttcctgc atccattgca cttcctccac ctatagatat
     3541 tattacatct ggattaaatt ctctcatagc tttagcgcct atcttagcac attccaaagt
     3601 tgggtctggt tctacttcaa agaatgtagt gtacttaata ttcatttcat ctaataaatc
     3661 agtaactgct ttagtatatc cattattgta aaggaatgta tctgttacaa taaaagctct
     3721 cttcttattc attacatctt taagttcttt taatgctact cctaaacaac cttttttgaa
     3781 ataaactttt tctggtgctc taaaccaaag catattttct ctcctctcag ctactgtttt
     3841 aatattaagt aaatgcttaa ctcctacatt ttcagatact gaatttccac cccaagaccc
     3901 acatcctaaa gtaagagaag gagctagttt aaaattatat aagtccccta taccaccttg
     3961 agaagaagga gtattaatta aaattctaca agttttcatg gctgaagtaa acttatcaag
     4021 tttttctctt tgatttacat catcaatata taatgaagat gtatgcccaa atccaccatc
     4081 ttctactaat ttttctgctt ttctaactgc atcatcaaaa tcacttgcct tgtacattgc
     4141 aagtactggt gaaagtttct catgagcaaa agcttcttct atttcaactg attctacttc
     4201 acctattaaa accttagtat ctactgggac ttcaaagcct gcaagttttg ctatagtaca
     4261 agctgtttgc ccaactattt tagaatttaa tcctccattt actaaaatta tatttcttat
     4321 tttttctgtt tcatctttat ctaataaata agcaccacgc tctttaaatt cttttttgac
     4381 ttcattgtat atattttcta atactataac cgattgttca gaagcacata tcataccatt
     4441 gtcaaaagtc ttagatacaa gaattgagtt tacagcagtt tttatatctg cactatcatc
     4501 aattattgct ggagtattac ctgcaccaac tccaatagct ggttttccag atgaatatgc
     4561 tgatttaacc attccaggac ctcctgtagc aagggtcaaa tccacttctt tcataacagt
     4621 tgtagttaat tctaaagatg gctcatctat ccatcctatt ataccttcag gagctccagc
     4681 taaaacagca gcctcaagaa caacttttgc agcctctata gtagaatttt ttgctcttgg
     4741 atgtggagag aaaattattc catttctagt ttttaaagct aaaagtgctt taaagatagc
     4801 agtagaagtt ggattagttg taggtacaac tgctgcaata acacctattg gttcagcaat
     4861 tttagtcata ccaaatgctt cgtctttctc tataacacca caagttttag tttctttata
     4921 tgcattatat atatactctg aggcataatg attctttata accttatctt cagcaattcc
     4981 cataccagtt tctttttgtg ccattatagc tagaggaact ctttgcttat tagctgccaa
     5041 agatgctgct aaaaaaattt tatctacctg ctcttgacta tatgtagcaa atattttttg
     5101 agcttctctg atttttgcta gttttttaac taagctttca acgttattta cagtatctaa
     5161 accaactgac ataacttcat tttctttttt attttctatt attacttgtt ctttatgagc
     5221 cattttaaca acctcctgag atagtttttt atttattatg ttaatttatt aacattatag
     5281 catttacttt tcctattttc aagttatttt tttaacattt
//
LOCUS       DAELLV010000229         5311 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.229, whole genome shotgun sequence.
ACCESSION   DAELLV010000229 DAELLV010000000
VERSION     DAELLV010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5311
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.229"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(624..2759)
                     /gene="rnr"
                     /locus_tag="KPD58_003148"
     CDS             complement(624..2759)
                     /gene="rnr"
                     /locus_tag="KPD58_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBG0266879.1"
                     /translation="MIPGLKESLLGLINESAYNPLKKEELAEIFDIHSSEMPMFYNFL
                     NELEEDGYICFTKKGKIVSPNQMGYFVGKFVSHKKGFGFVESDVEYTQDLFISSDNIN
                     GAMHNDRVMAEIVVPATEEKRAEGRIVKVIKREITRIVGTFQSSKTFGFVTPDNKKFT
                     KDIYIPKKHFSGAVDNDKVVCEITVWPQEDRKPEGKIIEILGQKGERGVEIDSIIREH
                     GLPEEFPKKVLQEAEAVAVEIPQEEIKRRRDLRDLNIFTIDGDDAKDLDDAISIEVLP
                     NGNFKLGVHIADVTHYVREKNKLDKEALKRATSVYLVDKVIPMLPKTLSNGVCSLNPF
                     EDKLTLSIFMEIDHKGNVVKHEICESVINSKARMTYTEVSDILEKDDEKLKKTFEHVV
                     EDFKNAEILARILMSRREKRGAIDFNFPEAKIILNGKGEVVDIKPYERRISNKIIEEF
                     MLISNETIAEHFYWMGIPFVYRIHETPSLEKMEELSKFISTFGYIIKGDKEEVHPKAL
                     QGIIEKIKGKKEEGAISTIMLRSLKQAKYSPECSGHFGLAAKFYCHFTSPIRRYPDLQ
                     IHRIIKESLNNKISGKRQEQLTTIVDYASVQSSEKERKAELAERDVHDFYKALYMKDK
                     VGQEFEGVVSSVTSFGIFVELDNTIEGLIRLANMNDDYYIYDESSYSVLGERTKKSYK
                     IGDLVKIKVESVNVDFKEIDFEIVEKIEE"
     gene            3355..3969
                     /locus_tag="KPD58_003149"
     CDS             3355..3969
                     /locus_tag="KPD58_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBG0266880.1"
                     /translation="MEEVIAKIITIENGFKEIENIAKKIVKNNDSKKCYYLSIYLYRS
                     EYYQVRELAVFILGFISVTISEALLFLKDNVSKDENWRVQEILAKSFDYYCSEVGYEK
                     ALPVIKEWLNSDSPNIKRAVTEGLRIWTSRDYFKSNPDVAISLLSSLKEDDSEYLRKS
                     VGNALRDISKKYPDLVKKELDSWDISNKKVEFVYKLASKCILAK"
     gene            3987..4349
                     /locus_tag="KPD58_003150"
     CDS             3987..4349
                     /locus_tag="KPD58_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0266881.1"
                     /translation="MKLRDEYTCPLELTHDMIKGKWKSIILWRLRIGPTSLSKLEKEI
                     NGINQKMLLEQLKELIEFGLVDKKTYEGYPLKVEYSLTPIRGKQILDALAIMQDIGIS
                     FMLDNGMEDILKEKGVLK"
     gene            4455..4952
                     /locus_tag="KPD58_003151"
     CDS             4455..4952
                     /locus_tag="KPD58_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HBG0266882.1"
                     /translation="MKVTSTGIVNGVIEDKYGKRGTQFNEGGMPTYSLPLKFEDAPKN
                     TVSFAVFLEDKDSVPVCGFAWIHWLAVNITKDELNENESVTATDFVQGTTSWHGKLGG
                     LDRLEASVYGGMAPPDRPHVYEIHVFALDTILDLEKGFYMNEFFKAIEGHVLDTFTLK
                     GSYSN"
BASE COUNT         1743 a          807 c          620 g         2141 t
ORIGIN      
        1 ttctcaatta cttatttttc aattagccac ttctattaag catttcttaa ttacttattt
       61 ttcaattagt cacttctatt aaacatttct taattattta tttttcaatt agccacttct
      121 attaagcatt tcttaattat ttatttttca attagccact tctattaagc atttcttaat
      181 tacttatttt tcaattagcc acttctatta agcatttctc aattacttat ttttcaatta
      241 gccacttcta ttaagcattt ctcaattact tatttttcaa ttagccactt ctattaagca
      301 tttcttaatt acttattttt caattagcca cttctattaa acatttctta attatttatt
      361 tttcagttgg ctttttctat taaatgtttc ttaattactt atctttcagt tagctctttc
      421 tattaaacat ttctcaatta cttatttttc aattagctac tttcattaaa tatttcttaa
      481 ttatttatct ttcagttaac tatttccatt agatatttct caattaccta tttttatcat
      541 ttattttata gagtattgtg cactatatat ttatacatat cataaatact acaacttgtt
      601 atctcttatt ttagctgttt tatctactcc tctatttttt caactatctc aaagtcaatt
      661 tctttaaaat ctacattaac actttcaacc tttatcttaa caaggtctcc tatcttatat
      721 gacttcttag ttctttctcc taaaacacta taagaactct catcataaat atagtaatca
      781 tcattcatat ttgcaagtct tatcaatcct tctatagtat tatctaactc aacaaatatt
      841 ccaaatgaag ttacgctaga tacaacaccc tcaaattctt gaccaacttt atccttcata
      901 taaagagcct tatagaaatc atgtacatct ctttcagcta actctgcctt tctttctttc
      961 tcggatgatt gaacagatgc ataatccact atagtagtaa gctgttcttg tctttttcca
     1021 cttattttat tatttaaaga ttcttttatt attctatgaa tttgaaggtc aggatatctt
     1081 cttattggtg aagtaaagtg acagtagaat ttagctgcta atccaaaatg tccactacat
     1141 tctggtgagt acttagcttg ttttaaagaa cgaagcatta tagtactgat agctccttct
     1201 tcttttttgc cctttatttt ctctattata ccttgtaatg cttttggatg tacttcctct
     1261 ttatcacctt ttattatata tccaaatgta gatataaact tactcaattc ttccattttt
     1321 tccaaagatg gtgtctcatg tattctatac acaaacggta ttcccatcca gtaaaaatgt
     1381 tcggcaattg tctcattact tataagcata aattcttcta ttatcttatt agatattctt
     1441 ctttcatatg gctttatatc tacaacttcg ccttttccat ttagaattat ctttgcctct
     1501 ggaaaattga agtctatagc tcctcttttt tcccttcttg acataagaat cctagctaaa
     1561 atttcagcat ttttaaaatc ttcaacaaca tgttcaaaag tctttttaag tttttcatca
     1621 tctttttcta atatatcaga tacttctgta taagtcattc tagcttttga atttattaca
     1681 gattcacata tttcatgttt aaccacattt cctttatggt ctatttccat aaatatagaa
     1741 agagttaact tatcttcaaa tggatttaag ctacatactc catttgaaag agtttttggc
     1801 aacattggaa ttactttatc tactaaataa actgatgtag ctctttttaa ggcttccttg
     1861 tcaagtttat ttttctctct tacataatgt gtaacatcag ctatgtgaac tcctagtttg
     1921 aagtttccat taggtaatac ttctattgat atggcatcat ctaagtcttt tgcgtcatct
     1981 ccatctatag taaatatgtt taaatctctt aaatctcttc ttctttttat ctcttcttga
     2041 ggaatctcaa cagctacagc ttctgcttct tgcaatactt tctttggaaa ttcttctgga
     2101 agaccatgtt ctcttattat agaatctatt tctacgcctc tttcaccttt ttgacctagt
     2161 atctctataa ttttgccttc cggttttcta tcctcttgtg gccatactgt aatttcacat
     2221 actaccttat cattatctac tgctccacta aaatgttttt taggtatata tatatcttta
     2281 gtaaatttct tgttgtctgg tgttacaaat ccaaaagttt tacttgattg gaaggtacct
     2341 actattctag ttatttctct ctttattact tttactattc ttccttctgc tctcttttct
     2401 tcagttgcag gtactactat ttctgccata actctatcat tatgcatagc accatttata
     2461 ttgtcacttg agataaataa atcctgtgta tattcaacat cagattctac aaatccaaaa
     2521 ccctttttgt gtgatacaaa ttttccaaca aagtatccca tttgattggg agatactatt
     2581 tttccttttt tagtaaagca gatataacca tcttcttcta attcatttaa aaaattataa
     2641 aacattggca tttctgatga atgtatatca aatatttctg ctaattcttc tttttttaga
     2701 ggattatatg cactttcatt tattaatcct agcaaacttt ctttaagtcc tggtatcatt
     2761 tttccctcct gttttacttt tcattatatt attatttata ttgtctttta tatttcttca
     2821 gacaaaacat tttttactta tcacataatt ttctatattt ccttattatt attacaagtt
     2881 aaaattactt atttatattt ttgatttaat gtaagctttg tcatactaac ttcaaacaaa
     2941 ttattatgtt ttataaatta tttgtaaata tcattataat tacttaagtt attctttatc
     3001 ataattgctt aaattactct ttatcaccta attatacacc atcaaatagg attggtaaaa
     3061 tattcatctc atataagtat tactatataa taatttattc tattagtata ctcaaatcat
     3121 gtaaaattta tataactcaa ttaatttttt aaatacaact ttataatata aaacttataa
     3181 taaaagtaaa tttatatgtt aaatgaggtt gattttttat tattttttat ttatgctagt
     3241 actaaagact tgcaaacgtt ttttgacaag tctttatttt gtctaaatta aacaaacttt
     3301 tttacaaata aatacataaa atttaaacta atctatttat tggaggagat aaaattggaa
     3361 gaagtaattg caaaaattat tactatagaa aatggattca aagaaattga aaacattgct
     3421 aaaaaaattg taaaaaataa tgactcaaaa aagtgttatt atctatcaat ctacttatat
     3481 aggtcagaat attatcaagt aagagaattg gctgtgttta tacttggttt tatatcagta
     3541 actatttctg aagctttgtt atttttaaaa gataatgtga gtaaagatga aaactggaga
     3601 gtacaagaaa tactagctaa atcatttgat tattattgca gtgaagtagg atatgaaaag
     3661 gctcttcctg ttataaaaga atggcttaat tctgatagtc caaacataaa aagggctgtg
     3721 acagaaggtc ttcgaatttg gactagtaga gactatttta aatctaatcc tgacgttgct
     3781 atatcactac tttctagttt aaaagaagat gatagcgaat acttgagaaa gtctgtaggt
     3841 aatgctctta gagatataag taaaaaatat ccagatttag taaaaaaaga attagattca
     3901 tgggatatat caaataaaaa agttgagttt gtatataagc ttgcttcaaa atgtatcttg
     3961 gcaaagtaaa atggaggtga acttatttga aacttcgtga tgagtatact tgcccattag
     4021 aactaacaca tgatatgata aaaggcaaat ggaaatctat tatattatgg agattacgca
     4081 taggaccaac ttccttatca aaacttgaaa aggaaatcaa tggtattaat caaaagatgt
     4141 tacttgaaca acttaaagaa ttaattgaat ttggtcttgt agataaaaag acatatgaag
     4201 gttacccact taaagtcgaa tactcactta cacctattcg agggaaacaa atattggatg
     4261 ctttggctat aatgcaagat ataggaatta gctttatgtt ggataatggt atggaagata
     4321 ttcttaagga aaaaggtgtt ttaaagtaaa tagataccca caaaaaagta agtaatttac
     4381 cattattttc aactggtata taattaaatt atagatatat tctattaatt tattataata
     4441 ggaggtaaat tattatgaaa gtaactagta caggtatagt aaatggtgtt atagaagata
     4501 aatacggtaa aagaggaact cagttcaatg aaggtggtat gcctacctat tcactacctc
     4561 taaagtttga agatgctcca aagaatacag tatcctttgc tgtattttta gaagataaag
     4621 actctgttcc tgtatgtggg tttgcatgga tacattggtt agctgtaaat ataacaaaag
     4681 atgagttaaa tgaaaatgaa agtgtaacag ctactgattt tgtacaaggg actactagtt
     4741 ggcatggtaa actaggaggt cttgacagat tagaggccag tgtatatggt ggaatggctc
     4801 ctccagacag acctcatgtc tatgaaatac atgtatttgc acttgatact atccttgacc
     4861 ttgaaaaagg attctatatg aatgaatttt tcaaagctat tgaagggcat gttttagata
     4921 catttacact aaaaggaagt tattctaatt aattaggttg actatagcta atcattaatt
     4981 aaatgctaca tttaattgat gattagctta attgttatta gaatattttc tgcactttat
     5041 cttaatatat attttagttg actggtattt taatttatat tagattatta tcaatttata
     5101 taaattgatt ttagcttaaa acttacttat ctatttcagt ttctattaga attattatta
     5161 gactcattaa aataaaagca ttaatataac taataatata tgttaaattc attaactcta
     5221 cataaagttt tataatctaa atgaaataca ataatctgca taaaattcaa tagcttatat
     5281 aaaatttaat aatttatata aatattatca a
//
LOCUS       DAELLV010000230         5296 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.230, whole genome shotgun sequence.
ACCESSION   DAELLV010000230 DAELLV010000000
VERSION     DAELLV010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5296
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.230"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(28..1431)
                     /locus_tag="KPD58_003152"
     CDS             complement(28..1431)
                     /locus_tag="KPD58_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG0266883.1"
                     /translation="MEEKYKEPLEYEKVSKLLTKYAIPSIIAMLVSALYNIVDQIFIG
                     QGVGVLGNAATNVTFPLTTICTAIALLLGIGGASKCSLELGAKNSEKATKAAGNSICL
                     MAVFGISLFIVVSIFLTPLLKFFGSTSEILPYAHTYTKITSIGLPFLIMSTGMSKLIL
                     ADGRPKASMFCMLIGAVINTILNPLFIFVFNMGIAGSALATVIGQLISFGISVHYIKN
                     FQHIKLHKKSFKLDSIIYKHIFALGSSSCFNQLAMTVTQIVMNNTLAYYGARSVYGSE
                     IPLACVGIIIKVNMIFISIIIGISQGMQPIIGYNYGAEKYNRVKEAYKFAVGSATIIS
                     VIAFLCFQLFPRQITSIFGNGNETYFIFAEHYFRTFLFLTFINGVQIVSSTFFTSIGK
                     SVLGLFTSLTRQILFLIPLIVILPMFMGIDGVMFAGPIADAAAAMVCIYFAVHEMKSL
                     TIKQNNLMSNHNKIAEV"
     gene            complement(1415..1939)
                     /locus_tag="KPD58_003153"
     CDS             complement(1415..1939)
                     /locus_tag="KPD58_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0266884.1"
                     /translation="MHEQIDCCERKKRTIDIKGENMRELLKWVSIINRYNTNYIDRKL
                     ADIGINNSQYFYVLHICNNPGITQDTIFEKILVNPSNITRALANLEKEGFITREPGVK
                     DKRTWHLYPTEKSLGCYDEILKITNGYVEELLSSFEKEEQELFMSMLKKVAFLAIDMN
                     EEAKLEAKENGRKI"
     gene            2219..2734
                     /locus_tag="KPD58_003154"
     CDS             2219..2734
                     /locus_tag="KPD58_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBG0266885.1"
                     /translation="MLEYIILGFLMEKELSGYDLKQIMSESTSYFFDASFGSIYPALK
                     RLETKGYIHYHEVIDGNKLKKLYSITNTGKEVFLEWLKKPINFSKTKQDHLVNIFFYK
                     YLPKEIIETNLKMLITEVELLLNKLSKQKNDAEENHCVDQYTYMYATMIYGVDYYNFV
                     INWCKDLLKNL"
     gene            2783..3286
                     /locus_tag="KPD58_003155"
     CDS             2783..3286
                     /locus_tag="KPD58_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG0266886.1"
                     /translation="MRLLIHDLTDKELEKLLPKLDDNIKILPNTKEIHKCIGCFGCWI
                     KTPSECVVKDYYSNMGKLINDSDGIIIISRCCYGGFSPFVKNVLDKSINYILPYFVIK
                     DNMMRHKSRYDKQINLKAIFYGDCITEAEKETATKLVNGNAINFNIKEHSVCFYENIQ
                     DIRGESI"
     gene            3283..3933
                     /locus_tag="KPD58_003156"
     CDS             3283..3933
                     /locus_tag="KPD58_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266887.1"
                     /translation="MNIVMINGSPKVNKNNSQYFLSELKQLIQNDNKVFTFKVDNTSK
                     YPDALNAILNCDRLIIASPLYVDGIPSHLLHFLEKCEKFLKTQNKKPIPVYAIVNCGF
                     NEGEHNHLAIQMIEHWCKKINFTWVQGIGIGAGEMFGSLESVPLGKGPKKDLGIALKE
                     LSKNILSDNIHIDDSNKSTIFISPNFPRFLYKLMGNHQWNTQAKSNGLTKSDILKK"
     gene            complement(4068..4847)
                     /locus_tag="KPD58_003157"
     CDS             complement(4068..4847)
                     /locus_tag="KPD58_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG0266888.1"
                     /translation="MNDTVKEKLAESLTSDTTDILPYLPYLLQDFWELGSSPEQMLQL
                     FRKHIPFNEDTKVLDLACGKGAVSVKLAKELNIHIKGIDLIPEFIDYARRKAIEYKVD
                     ALCDFEMNDANSSVKIEHDYDCVIFGAVGDILGNYKETISKLLPTIKSKGFILIDDAY
                     VVDKSTNEQLNFERVYPTYNEWCILFKELGLKLIECIPCLELENDVEEEMNWIITRAN
                     ELIKKYPSQKDMFEQYVISQKNEYTDLQQDLVGATWILQKE"
     gene            complement(4959..5210)
                     /locus_tag="KPD58_003158"
     CDS             complement(4959..5210)
                     /locus_tag="KPD58_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HBG0266889.1"
                     /translation="MNVENELLKNLDRLHTTELGVVRIKKNLSLETNDVVNWCKTKIE
                     SPNAIINRKGKNWYISVYDCIITVNAHSYTIITAHKEKK"
BASE COUNT         1901 a          851 c          638 g         1906 t
ORIGIN      
        1 aattttctat acttcttaaa tattgtctta tacctctgca atcttattat ggttactcat
       61 caaattattt tgctttatag tcaatgattt catttcatga actgcaaagt atatacatac
      121 cattgccgct gctgcatctg ctattggtcc tgcaaacatt actccatcaa ttcccataaa
      181 cattggtaaa attactatca atggaattaa aaataaaatt tgacgagtca aagaagtaaa
      241 aagtcccaac actgatttcc caattgatgt aaagaaagtt gatgatacta tttgaacacc
      301 atttataaat gttaagaaca agaaagttct aaaataatgt tctgcaaata taaaataagt
      361 ctcatttccg ttaccaaata tagacgtaat ttgtcttggg aataattgga aacataagaa
      421 tgcaataaca gatataatag ttgcacttcc cactgcaaac ttatatgctt cttttacacg
      481 attatacttt tcagccccat aattataccc tataattggt tgcatacctt gtgatatacc
      541 aataattata gatataaaaa tcatatttac tttaataatg ataccgacac aagcaagtgg
      601 aatctcactt ccataaactg acctagcccc ataatatgcc aatgtattat tcataactat
      661 ctgtgttaca gtcattgcta attgattaaa acatgagctt gaacctagcg caaatatatg
      721 tttataaatt atactatcta atttaaaact tttcttatgt aatttaatat gttgaaaatt
      781 ctttatataa tgaacactta ttccaaaaga tataagttgc ccaataacag ttgctaaagc
      841 agacccagca attcccatat taaaaacaaa aatgaataat ggattcaaaa ttgtattaat
      901 aactgctcct attaacatac aaaacataga agctttcggt ctcccatctg caagtatcaa
      961 cttactcatt ccagtggaca ttataagaaa tggtaatcca attgatgtaa tttttgtata
     1021 tgtgtgtgca taaggtagta tttctgatgt agagccaaag aattttaata gtggtgttaa
     1081 aaatattgat actactataa ataaacttat tccaaaaaca gccatcaagc atatagaatt
     1141 tcctgctgct tttgtagctt tttctgaatt tttagcacct aattccaatg aacacttaga
     1201 cgctcctcca attcctaaca ataatgcaat tgcagtacat attgttgtta gtggaaaagt
     1261 gacatttgta gcagcattgc ccaatacacc aacaccttgc ccaataaata tttggtcaac
     1321 aatgttatat aaagcactta ctaacattgc tataatacta ggaattgcgt acttagttaa
     1381 taactttgaa accttttcat attccaatgg ctccttatat ttttcttcca ttttccttcg
     1441 cctccaattt tgcttcttca ttcatatcaa tagccaaaaa tgcaactttc tttaacattg
     1501 acataaacaa ctcttgctct tctttctcaa atgaactcaa caactcctca acatacccat
     1561 ttgttatttt tagtatttca tcataacatc ctaaagattt ttctgtagga tataaatgcc
     1621 atgttctttt atcctttaca cctggttctc ttgttataaa gccttctttt tctaaatttg
     1681 ccaaagctct tgttatatta ctaggattca caagaatttt ctcaaatata gtgtcctgtg
     1741 taatacctgg attattacat atgtgtaata cataaaaata ttggctatta ttgattccaa
     1801 tatccgccaa tttcctgtca atataatttg tattgtatct atttataatg gaaacccatt
     1861 ttaatagctc tctcatattc tctcctttaa tatctattgt cctttttttg cgttcgcaac
     1921 aatctatttg ctcatgcaat tattctactg ctaattttat actttgtcaa tagcacacaa
     1981 gtttatattt aaaccataaa gttactataa attttatata ttagactctt actttgattt
     2041 aactattttt aaatctttat tgatatttaa ttacctttta tattaatttt tttgcatatt
     2101 tatcaataac tcctaattct ttttagctta tcttaaaaag catcttgacg taagatttat
     2161 tatctgtaat aataaatata tcgaatcgat atatatctaa ttgataccag gaggtaatat
     2221 gttagaatac attattttag gatttttaat ggaaaaagaa ttaagtggat acgacttaaa
     2281 acaaataatg tcagaaagta cctcgtactt ttttgacgcc agctttggaa gtatctatcc
     2341 tgcacttaaa agattagaaa caaaaggata tatacactat cacgaagtta tagatggtaa
     2401 caaattaaaa aaactatatt ccataactaa tactggtaaa gaagtatttc ttgaatggtt
     2461 aaagaaacct ataaattttt ctaaaacaaa gcaagaccac cttgttaata tattctttta
     2521 caagtatcta cccaaagaaa taattgaaac aaacttaaaa atgttaataa ctgaagtaga
     2581 attacttcta aataaattaa gtaaacaaaa aaatgatgca gaagaaaatc attgtgttga
     2641 ccaatataca tatatgtatg caacaatgat ttatggagtt gactattata attttgttat
     2701 taattggtgt aaagatttac tgaaaaatct ataagatata atttttagaa gtaaacatac
     2761 ataataaaag gagatggact ttatgaggct tttaatacat gatttaactg acaaagaatt
     2821 agaaaagtta ttacccaaac ttgatgacaa tataaaaata cttccaaaca caaaagaaat
     2881 ccacaaatgt attggttgtt ttggttgctg gattaaaact ccttctgaat gtgttgtaaa
     2941 agattattac agcaacatgg gtaaacttat aaatgactct gatggaatta ttataataag
     3001 cagatgctgt tacggagggt ttagcccttt tgtcaaaaac gtacttgata aaagcattaa
     3061 ttatattctt ccatactttg taataaaaga taatatgatg cgtcataaaa gcagatatga
     3121 taaacagata aatttaaaag ctatattcta tggagattgt attacagaag ctgaaaaaga
     3181 aactgctacg aaattagtaa atggaaatgc tataaatttc aatataaaag aacactctgt
     3241 ttgtttctat gaaaatatac aagatataag aggtgaatcc atatgaatat agtaatgata
     3301 aatggtagtc caaaagtaaa taaaaataat tcacagtatt ttttatctga attaaaacaa
     3361 cttattcaaa atgataataa agtttttaca tttaaagtag ataacacatc taagtatcct
     3421 gacgctctta atgccatact taattgtgat agattaataa tcgcatctcc tctttatgta
     3481 gatggtattc catctcattt acttcatttt ttagagaaat gtgaaaaatt tttaaaaaca
     3541 caaaataaaa aacctattcc agtgtatgca atagttaact gtggattcaa tgaaggtgaa
     3601 cataatcatt tggcaattca aatgatagag cattggtgta aaaaaatcaa ctttacatgg
     3661 gttcaaggta ttggaattgg agctggggag atgtttggtt cactagaatc cgtacctttg
     3721 ggtaaaggac ctaaaaaaga tttaggaatt gcattaaaag aactttcaaa aaatatactt
     3781 agtgataata tacatataga tgattccaac aaaagtacta tttttatttc acctaacttc
     3841 cctagattcc tatataaact tatggggaat catcaatgga atactcaagc taaatcaaat
     3901 ggattgacta aaagtgatat actaaagaaa taaaatttta cttcaacaaa tactaaaaac
     3961 cgtcaagcac gaataaacta ttcatcttga cggtttttta tgttatttat attcactaga
     4021 catgggataa ttcactataa cattgattat ctagtctaat atttatctta ttctttttgt
     4081 aaaatccaag ttgctccaac aaggtcctgc tgtaaatcag tatattcatt tttttggcta
     4141 ataacatatt gttcaaacat atctttttga gaaggatatt ttttaattag ctcatttgca
     4201 cgagttataa tccaattcat ttcctcttca acatcatttt caagttctaa acatggtata
     4261 cattcaatta gcttcagacc taattctttg aacaatatac accactcatt gtaagtggga
     4321 tacaccctct caaaattaag ctgttcattt gtagatttat ctactacata tgcatcatca
     4381 attaaaatga accccttact ttttattgta ggtaatagct tacttatagt ttctttataa
     4441 ttacctaaaa tatcccctac agctccaaaa atcacacaat cataatcatg ctcaattttt
     4501 acagaactat tagcatcatt catttcaaaa tcacacaaag catcaacctt atattcaata
     4561 gcctttcttc tagcataatc aataaattca ggtatcaaat caataccttt gatatgtata
     4621 ttcaactctt ttgcaagctt cacagataca gcaccttttc cacaagctaa atccaaaact
     4681 tttgtatcct cattaaaagg tatatgcttt ctaaataatt gtaacatttg ttctggtgaa
     4741 cttcctaatt cccaaaaatc ttgtaataaa taaggcaaat aaggtaaaat atcagtagta
     4801 tcagaagtta gtgactctgc caatttttct tttacagtat cattcatttt ttcaacctcc
     4861 aaaatttaaa ttattattca aaagtaatat tattttatat atttcttcat aggtactaca
     4921 aacagtggaa aatgcttttg acctttgtat tttgcaattc atttcttttc tttatgagct
     4981 gtaataattg tataactgtg agcgtttact gttataatac aatcatatac acttatatac
     5041 cagttttttc cctttctatt aataatggca tttggagatt ctattttagt tttacaccag
     5101 ttcactacat catttgtctc caaagaaaga tttttcttaa ttcttacaac gcctaactct
     5161 gttgtatgca atctatctag gtttttaagt aattcgtttt ctacattcat ttttattaaa
     5221 acactttatc ctttgcaaaa ttaaatctat tatttagtac tattttatca tagtattgtg
     5281 tattttatca taatct
//
LOCUS       DAELLV010000231         5267 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.231, whole genome shotgun sequence.
ACCESSION   DAELLV010000231 DAELLV010000000
VERSION     DAELLV010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5267
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.231"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            373..1527
                     /locus_tag="KPD58_003159"
     CDS             373..1527
                     /locus_tag="KPD58_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG0266890.1"
                     /translation="MSNNINQDLETKIIHWGHSADPTTGALATPICQTATFAAKTVEH
                     FEELCMTWGYVYTRECNPTLTELEAKLAMLENAESAISSTSGMGAITSTILALVKSGD
                     HIVSSDGIFSHTKLFMSELLSKFGVEVTFVDAVNPQNVKEAMRPNTKIVYIESPLNPS
                     LDLVDIKTIAEIAHENKSLAIVDSTFGTPIVQRPIDLGADLVIHSLTKFINGHGDTLG
                     GAVAGSKELIDLVRWPSLCCFTGASLPPMSAWMILRGMKTLDMRMKKHCENGLAVAEF
                     LEEEENVELVKYPALKSHPQYELCKTQMNGLGGGVVSFKLKDGINGLTRDQASRRLMN
                     SLELATIATSLGEEHTLVQMNGENLIRIAVGLESSNDIINDFKQAIEKLK"
     gene            1578..2831
                     /locus_tag="KPD58_003160"
     CDS             1578..2831
                     /locus_tag="KPD58_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0266891.1"
                     /translation="MESKNNVKRFLIIFILAFGTTAMYSLPYMKSSFYDPMQQALALS
                     HTQIGNLLSLYGLVGMVSYFIGGWFADRFSVRKLITFSLIASGILGFYFSTFPSYSMI
                     LLIFVLWGFTTILTFFSASVKVVRMQGSESEQGRIFGFYEGLSGVSGTLISFIGLYFF
                     GKFAEITVGFKYVVWLYSAASIICGILLFFLVEEKKDSGASDEGLSIKSLLKVVTMPK
                     AWLIGLIIFSTYLVFSSLTYLSPYLSEVYVMPMTLVSALSIIRTYVIKMGASPVAGVI
                     TDKVGSSIRVMFVGFILMTVSTAAYLVIPKSTGFIWIAVINMIILSVILFGFRGIYFA
                     SVSESNISLETTGAVVGFASFIGFSPDAFYYTIAGNWLDKYGQTGYTYIFILSVVCAV
                     IGIFATYALNKINKRENKGLNNKIA"
     gene            2957..4351
                     /locus_tag="KPD58_003161"
     CDS             2957..4351
                     /locus_tag="KPD58_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HBG0266892.1"
                     /translation="MLTFWENGIIVIFAFILLGIGYYFSRNVKDMESYYLGNRSLPWS
                     LIVGTLVASWYGGVGTVGSVEYAAIYGISAWAVWSIAAHIGRMPLALWVGPRIHIRTN
                     ITVPDLLESFYGKHVAIIGAILMFIYCAQLGEITAMGTIGNVAWKIDKELIGLIMVGV
                     VVVLTVLGGLMGVAITDMVLFFCMVFGLTMVMPGQFEQIGGFQGLTEALKDAPQLMHP
                     TKGMSAMKALMLIVYSFGAYSDPTFYQRFSAADSPKVGRRALLTCFSLWICFDMVLTM
                     TGLIVRATYPEMAPAAGYITLVLDTLPQGIRVIFIIGILGSIISTLDSYYLAAGATLA
                     NDIYGRITKKELSQKQLVKFTRIGVCLAAVMGLSVAFRFENVYDACIFVGSIWMGSAF
                     VPIVGGLLGNGRRTSMGGILGMIVGGVTFGYFKIFPVQSFELEPLVIAIPFSFIAWMI
                     GNRIGENLNTQNNL"
     gene            4366..4596
                     /locus_tag="KPD58_003162"
     CDS             4366..4596
                     /locus_tag="KPD58_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266893.1"
                     /translation="MSKLVSKVKKDLEIKWLGIDGFLVLLYFLITGFIFYGILTRNDI
                     IVGKIIPYGVMSVVIVIFFDYIYRVLKMTRKK"
     gene            4613..5143
                     /locus_tag="KPD58_003163"
     CDS             4613..5143
                     /locus_tag="KPD58_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266894.1"
                     /translation="MDIRALPIRITKEEAIEIAKSGKNSLFSKLILKNKYAHEVRLHY
                     IEFKIITLKIHKTKSIFKNRRYSNYINELKILVNGSTGSGSIVQDFPTYEVVKNVDCN
                     IVQYSDKDERRVKAKANKMAMRLTHRFMGGIPEIEIVKIESIFRPYWVVFFGKVELNR
                     RVSYLPIEADGFVIGM"
BASE COUNT         1914 a          546 c         1019 g         1788 t
ORIGIN      
        1 taatatattt atgaaaagat gataaatctt gaaaaatcta tttatgataa aaaaactaat
       61 gcttatatgt aattatttat attgtgatat aaaaataggt atatcttagg atgactttag
      121 ttattctaag atatacctat ttttgtactg actattttaa acattgtaaa aaaaatatca
      181 aaaacatatt gactctatag ttactataga gtttataatt agaactgtaa aaaaacaaaa
      241 taagtgtcat atttttagaa attataacaa ataaaaaagt atacttgaaa aaatgtatta
      301 agactaagct aaattgatga aatttggtag ggaaaacgtt gtataaataa gtcaaaatgg
      361 gaggaaatca aaatgagtaa caatattaat caagatttag agacaaaaat aatacattgg
      421 ggacatagtg cagacccaac aacaggtgca ttggctacac caatatgtca aacagcaaca
      481 tttgctgcaa agacagtaga gcattttgaa gagttgtgta tgacatgggg atatgtgtat
      541 acaagagagt gtaatcctac tcttacagag ttagaagcta agttggctat gcttgaaaat
      601 gcagaaagtg ccatatctag tacatcaggg atgggggcaa taacatctac tatattagca
      661 ttggtaaaaa gtggtgacca tatagttagt tcagatggaa tattttcaca cactaaatta
      721 ttcatgtctg agttgttatc taaatttgga gttgaagtta catttgtaga tgcagtaaat
      781 ccacaaaatg taaaagaagc aatgagacca aatactaaaa tagtttatat agaaagtcct
      841 ttaaatccat cattagactt agtagatata aaaacaatag cagaaattgc tcatgaaaat
      901 aaaagcttag ctatagttga tagtactttt gggacaccaa ttgtacaaag accaatagat
      961 ttaggtgcag atttagtaat acatagtctt actaagttta taaatggaca tggggatact
     1021 ttagggggag cagtagcagg ttcaaaagaa ttaatagatt tagttagatg gccaagttta
     1081 tgttgtttta caggagcatc tttaccacca atgagtgcat ggatgatttt aaggggtatg
     1141 aaaacgttag atatgaggat gaaaaagcat tgtgaaaatg gattagcagt agcagaattt
     1201 ttagaggaag aagagaatgt ggaattggta aagtatccag cattaaaatc tcatcctcaa
     1261 tatgagcttt gtaaaactca aatgaatggt ttaggtggag gtgtagtttc atttaaactt
     1321 aaggatggta tcaatggatt gacaagagac caagctagta gaagattaat gaattcttta
     1381 gagcttgcaa caatagcaac aagcttaggg gaagagcata cattagttca aatgaatggg
     1441 gaaaatctaa taagaatagc tgtaggatta gaatcttcaa atgatataat aaatgatttt
     1501 aaacaagcaa tagaaaaatt aaaataaaat tatatgaatg agattataaa caataaaatt
     1561 ttaaggagag agaatcaatg gaaagcaaaa ataatgttaa aagatttttg ataatattca
     1621 tattggcatt tggaacaaca gctatgtata gtttaccata tatgaaatca tcattttatg
     1681 acccaatgca acaagcttta gcattaagtc atacacaaat agggaactta ttgagtttat
     1741 atggattagt aggaatggta tcttatttta taggaggatg gtttgcagat agattctcag
     1801 ttagaaaatt aataactttt tctttaattg cttcaggaat acttggattt tatttctcta
     1861 cattcccatc atatagtatg atacttttaa tatttgtatt atggggattt acaacaatat
     1921 taactttctt ctcagcctct gttaaggttg ttagaatgca aggaagtgag tcagagcaag
     1981 gaagaatatt tggtttttat gaagggttat caggtgtatc aggtacatta atttctttta
     2041 taggtcttta cttctttggt aaatttgctg aaataacagt aggattcaaa tatgtagtat
     2101 ggttatattc tgcagcatct ataatatgtg gtatacttct atttttctta gtggaagaga
     2161 aaaaagatag tggagcatca gatgaaggat taagtattaa gtcattgtta aaagtagtta
     2221 caatgccaaa ggcttggcta attggtctta taatattttc aacttattta gtattctcaa
     2281 gtttaactta tttaagtcca tacttatcag aagtttatgt aatgccaatg actctagttt
     2341 ctgctttatc aataataaga acttatgtta taaaaatggg agcatctcca gttgctggtg
     2401 ttataacaga taaagttggt tcatctataa gagtaatgtt tgtaggattt atattaatga
     2461 cagtaagtac agctgcttac ttggttattc ctaagagtac aggatttata tggatagctg
     2521 taataaacat gataatacta agtgtaattt tatttggatt tagaggtata tactttgcat
     2581 ctgtttcaga gtctaatata tctttagaaa ctacaggagc agtagttgga tttgcttcat
     2641 ttataggatt ttctccagat gcgttttatt acactattgc aggtaattgg ttagataaat
     2701 atggacaaac aggatataca tatatattta ttttatctgt agtatgtgca gttataggga
     2761 tatttgctac ttatgcttta aataaaataa ataaaagaga aaataaaggt ttaaacaata
     2821 aaattgctta ataacaagat tacttaaata cttataatta ttatataact ctacctattt
     2881 ttatggaaat gggtagagtt gttcatattt agatattaaa gttaaaaatt aaaatataag
     2941 aatgtggaga ttagatatgt taacattttg ggaaaatggg attatagtta tatttgcatt
     3001 tatattatta ggaataggtt attatttttc tagaaatgta aaagatatgg aaagttatta
     3061 cctaggaaat cgaagtttac catggtcctt aatagtagga actttggttg catcatggta
     3121 tggaggagtt ggtacagtag gtagtgtaga gtatgcggct atatatggaa tatctgcatg
     3181 ggcagtttgg tcaatagctg cacatattgg gagaatgcct ttagcattgt gggttggtcc
     3241 aaggattcat ataagaacca atataacagt tcctgattta ctggaaagtt tttatggcaa
     3301 gcatgttgct ataattgggg caattttgat gtttatatat tgtgcacaac ttggagaaat
     3361 aactgctatg ggaactatag gtaacgtagc atggaaaata gataaagaat taataggatt
     3421 aataatggta ggagtagtcg ttgtacttac agtacttggt gggcttatgg gagttgccat
     3481 tacagatatg gtattgtttt tttgtatggt atttggttta actatggtta tgccaggtca
     3541 atttgaacaa ataggagggt ttcaaggtct tacagaagca ctcaaagatg caccacagct
     3601 tatgcaccct acaaagggta tgtcagctat gaaagcattg atgttaattg tatattcttt
     3661 tggagcttat tcagacccaa ctttttatca aagattttca gcagcagatt ctcctaaggt
     3721 tggaagaaga gccttattaa cctgtttttc actatggata tgttttgaca tggtgttaac
     3781 tatgacagga cttatagtaa gagctactta tccagaaatg gctccagctg ctggatatat
     3841 aactttagtt ttagatacat taccacaagg aataagagta atatttataa taggaatttt
     3901 aggttctata atatcaacat tggatagcta ttatctagca gctggggcta cattagcaaa
     3961 tgatatatat ggaagaatta caaaaaaaga gttatctcag aaacaattag taaagtttac
     4021 tagaataggt gtatgtttag cagcagttat ggggcttagc gtagcattta gatttgaaaa
     4081 tgtatatgat gcatgtatat ttgtaggaag tatttggatg ggttcggcat ttgtacctat
     4141 agtaggtggg cttcttggaa atggaagaag aacatctatg ggtggtattt taggaatgat
     4201 tgttggtgga gtgacttttg gttattttaa aatatttcca gttcagagct ttgagttaga
     4261 gcctcttgtg atagctatac ctttttcatt tattgcatgg atgattggta atagaatagg
     4321 tgaaaattta aatacacaaa ataatctata aaaggagaaa ctgctatgtc taaattagtg
     4381 tctaaggtaa aaaaagattt agaaataaaa tggcttggaa tagacggttt tctagtatta
     4441 ttgtactttt taataacagg atttatattt tatggtatat tgactagaaa tgatattata
     4501 gttggaaaaa taattccata tggagttatg tcagttgtaa ttgtaatatt ttttgattat
     4561 atatatagag ttttaaaaat gacaagaaaa aaataagaaa gagaatgatt ttatggatat
     4621 aagagcgtta cctataagaa ttacaaaaga agaagctata gagatagcta aaagtggaaa
     4681 aaattcttta tttagtaagc taatattaaa gaacaaatat gctcatgaag ttagacttca
     4741 ttatatagag tttaaaatta tcacacttaa aattcacaaa acgaaatcca ttttcaagaa
     4801 taggagatat tctaattata taaatgaatt aaaaatttta gtgaatggtt ctacaggttc
     4861 tggtagtata gtacaagatt ttcctacata tgaagttgta aaaaatgtgg attgtaatat
     4921 tgtgcagtat agtgataagg acgaacgaag agtaaaagca aaggcaaata aaatggctat
     4981 gagattaact cataggttta tgggaggaat cccagagatt gaaatagtta aaattgaaag
     5041 tatttttaga ccatattggg tagtattttt tggaaaggtg gagttgaata gaagagttag
     5101 ttatcttcca attgaagctg atggttttgt tataggaatg tagtatattt tatataaata
     5161 tattttacac ttattgtata atacatattg agagaagata ttatatttag ttatataaga
     5221 tgttttctat aatagatgat atgatttaca ataagtgcat tttaatt
//
LOCUS       DAELLV010000232         5258 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.232, whole genome shotgun sequence.
ACCESSION   DAELLV010000232 DAELLV010000000
VERSION     DAELLV010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5258
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.232"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..596
                     /locus_tag="KPD58_003164"
     CDS             <1..596
                     /locus_tag="KPD58_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266895.1"
                     /translation="IKKDKLTDFIIEKNSNSFKIFTKCETIKSLTKNFTACYIKESIE
                     NSTNIKVCVGYGIGNDIKQAMSSAISANKESKINHLNYSFLVNENYETIGPLLCDKLL
                     TVSNKVTPHINTIAKSSKLSTLTIQKIISVTEILQRDEVTPKELSSCLNVTVRTANRF
                     LSSLLKSGNATILYEKQNSTKGRPERVYKLFISENKY"
     gene            complement(724..1914)
                     /locus_tag="KPD58_003165"
     CDS             complement(724..1914)
                     /locus_tag="KPD58_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0266896.1"
                     /translation="MKNFFKEAQSIEGEIIRWRRYLHENPEIGCNIPKTVTFVTSTLE
                     KMGYSPKLTSNNGIVAVLDSENGGKTLLLRADMDALPIMEETDLPFKSKNKDASHSCG
                     HDNHTAMLLGAAKILMDNKHLLKGRVKFVFEPDEETTRGAKSMIDDGILENPQVDAAM
                     AFHSMVGKFLNTGQVAYSRGPAMASADIFEITVEGKATHGASPEFGVDPINIMSHIQI
                     ALNTILSREKPQKEPVVLTIGMFNAGNVPNAIPNKASMSGTLRTFDQFVREKLKNRII
                     EISTGIAKSFGGNAKVEFKMGTPAVINDLDVGDEIVSYVKEVIGNENVIDFPSAMGSE
                     DFSEILLRVPGVFLWIGMGSISQGYNFGMHNPKAIFNEEGLVYGSALFAHCAVKWLEN
                     NFSR"
     gene            complement(2020..3399)
                     /locus_tag="KPD58_003166"
     CDS             complement(2020..3399)
                     /locus_tag="KPD58_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0266897.1"
                     /translation="MIGSISINEIDKKRFKLDDLSKKIWENPEKAFKEFKACENTANF
                     LRSEGFDVEVGVGGLATAIRASFGSGKPVIGFMGEFDALPGLNQKVSTKQEAFELGAY
                     GHGCGHNLLCTAHVGAVVGLKREMIENNLSGTIVFYACPGEEQLTGKGFMARGGAFEG
                     LDLAINFHPNKISEATIGTSTAVNSVKFHFKGKTAHAGSDPQNGRSALDAVELTNVGA
                     NYLREHVTSDVRIHYTITDGGVAPNIVPDKASVWYYTRALSREAVESTYERLVKVAKG
                     AAMMTETEVEVEFLGGCYNTLNNHVLANLVSECMNEIKQEPWTREELDFAAELDRQSP
                     QQYKAMISKYNLPEGTHLYYGGGNVTNFNSYGSTDIGDVMHIVPTAYFFTGCTNLGAP
                     GHSWQFTSCAGSSIGEKGMIYASKIMAMFGAKILNNPEIAKKAKEEFDKSMNGKTYKC
                     PIPDDIPTP"
     gene            complement(3430..4638)
                     /locus_tag="KPD58_003167"
     CDS             complement(3430..4638)
                     /locus_tag="KPD58_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266898.1"
                     /translation="MENFWNPLTCFTVCVMIYAFSEFLSKKTKGAISTLLFVCIIFLI
                     GFWTNILPKDITGASGILAAMSNFGIALLITNLGTLINLEDLCHEWKTVVIALVGIVG
                     IGVGCLTVGSMLFGREYALIAAPPIAGSTVAGIIVTTVAEAANRPELAAFVILVLSFQ
                     KFFGIPITTYCINKELKFKRDNGDFLKDESKSNFKLPSMRIFKEDLGKKSSTLVYLAK
                     LSFVAMIGNFVGLATLIPGFTPANYYLNPNIACLLVGLIFTRIGFLEKGILDKSQSNG
                     IIMFGCMLMLPGGLAKVTPTALFGMIVPTIGILVVGSVFIILFTAIVGKLLGYSPRMS
                     AAIGVTCMLAYPATQIISNEGIDAMEGTEEEKQRATDYLLPKMIIGGFVTVTIASVAF
                     ASIIGPIIFG"
BASE COUNT         1755 a          986 c          610 g         1907 t
ORIGIN      
        1 gtattaaaaa agataaatta accgatttta taattgaaaa aaatagtaat tcttttaaaa
       61 ttttcactaa atgtgaaacg attaaatcat taactaaaaa ttttactgca tgttatataa
      121 aagaatctat tgaaaatagt acaaatatta aagtatgtgt tggatatgga attggtaatg
      181 atataaagca agctatgtct agtgccataa gtgctaataa ggaatctaaa ataaatcatt
      241 taaattacag tttcttagtt aacgaaaatt atgagacaat aggaccttta ctgtgtgata
      301 agttacttac tgtttcaaat aaagtaactc cacatataaa tacaattgcc aaaagttcta
      361 aattgtctac tcttactatt caaaagatta tatctgttac tgaaattcta caaagagatg
      421 aagttactcc taaggaatta tcttcatgtt taaatgtaac agttagaact gctaatagat
      481 ttctaagtag tctcttaaag tctggaaacg ctactatatt atatgaaaaa caaaactcaa
      541 caaaaggtag acctgaaagg gtatacaaat tatttatatc agaaaataaa tattaaaatc
      601 caaaatgcat aaaaatattt tatcatctaa tattaagtaa aattatatat gactttctaa
      661 aaggtatatc tttgcatctt tttaagatat accttttatg atatttaata atattttatg
      721 ttattatcta ctaaaattat tttctaacca ttttactgca caatgagcaa ataaagctga
      781 accataaact aatccctctt cattaaaaat tgctttagga ttatgcatac caaaattata
      841 tccctgacta atacttccca ttccaatcca taaaaatact cctggaaccc ttagtaaaat
      901 ctctgaaaaa tcttctgaac ccattgcaga tggaaagtct attacatttt catttcctat
      961 aacttcttta acataagata caatctcatc tcctacatcc aaatcattta taactgcagg
     1021 agtccccatt ttaaattcca ctttagcatt tcctccaaat gatttagcta ttcctgtaga
     1081 tatttcaata attctatttt ttaatttttc tctaacaaat tggtcaaatg ttcttaaagt
     1141 tcctgacata gatgctttat ttggtatagc atttggtacg tttcctgcat taaacatacc
     1201 aatagttaaa actacaggtt ctttctgagg tttttctctt gaaagtatag tgtttaatgc
     1261 tatttgtata tgcgacatta tgtttattgg gtccactcca aattcagggc ttgctccatg
     1321 agttgctttt ccttctactg ttatttcaaa tatatccgca gatgccatag ctggaccacg
     1381 tgaataagct acttgacctg tgttaagaaa tttacccacc atactgtgaa atgccatagc
     1441 tgcatcgacc tgaggatttt ctaatatacc atcatcaatc attgattttg cacctctggt
     1501 agtctcttca tctggttcaa atacaaactt tactctacct ttaagtaagt gtttattatc
     1561 cataagtatt ttagctgctc ctaagagcat tgctgtgtga ttatcgtgtc cacaactatg
     1621 agatgcatct ttattcttac ttttaaaagg taaatccgtc tcctccataa taggaagtgc
     1681 atccatatct gcccttaata ataaggtctt tccaccattt tcactgtcta aaacagctac
     1741 tattccatta ttagacgtca atttcggaga atatcccatt ttttctaaag tactggtaac
     1801 aaaggttact gttttcggta tattgcaacc aatttcggga ttttcatgta aatatcttct
     1861 ccatctaata atttcccctt ctattgactg cgcttcttta aaaaaatttt tcatattcct
     1921 tcctcctaat cagatagtca gcaaaatctc actttctttt agtaatgaaa tgatgctgat
     1981 ataaaaatta taattttata tattaaattt catgtctttt taaggagttg gaatatcatc
     2041 tggaatagga catttatatg ttttaccatt catagattta tcaaattctt cttttgcctt
     2101 tttagctatc tctggattat ttaatatttt agctccaaac atagccataa tctttgatgc
     2161 atatatcata cctttttctc ctatagaact tcctgcacaa gatgtaaact gccagctatg
     2221 acctggtgct cctagattcg tacaccctgt aaaaaagtat gctgtcggaa ctatatgcat
     2281 tacatctcca atatcagtag aaccataact attaaagttt gtaacatttc ctccaccata
     2341 atatagatga gttccttctg gcaaattata tttacttatc atagccttat attgttgtgg
     2401 gctttgtcta tctaattcag ctgcaaaatc caattcctct cgagtccaag gctcttgttt
     2461 aatctcattc atacattctg atacaagatt tgctaaaaca tgattgttta aagtattata
     2521 acaacctcct aaaaactcta cttcaacttc agtttcagtc atcattgcag ctccttttgc
     2581 tacctttaca agtctttcat atgtactttc tacagcttct ctacttaaag ctcttgtata
     2641 ataccatact gatgctttat caggaactat gttgggtgct actcctccat ctgtaattgt
     2701 ataatgaatc cttacatcag aagtaacatg ttctctcaaa taattagctc ccacattagt
     2761 aagttccaca gcatctagtg cacttcttcc attttgaggg tctgagcctg catgcgcagt
     2821 ctttccctta aagtgaaact ttacagagtt aacagctgtt gaagtaccaa ttgtagcttc
     2881 actaatttta tttggatgaa aatttatagc taagtctaac ccttcaaacg ctccacctct
     2941 agccataaat cctttacctg ttaattgttc ttctcctgga catgcataaa atactatagt
     3001 cccacttaag ttattttcta tcatctctct tttaagccct actactgccc ctacatgtgc
     3061 tgtacataat aaattatgcc cacatccatg accatatgca cccaattcaa atgcttcttg
     3121 ctttgttgaa actttttgat ttaaaccagg taatgcatca aattctccca taaaacctat
     3181 aactggttta cctgaaccaa aactagctct aatagccgtg gctaaacctc ctacacctac
     3241 ttccacatca aatccttcac ttcttagaaa gtttgctgta ttttcacatg ctttaaattc
     3301 tttaaatgct ttttctggat tttcccatat tttcttactt aaatcatcaa gtttgaatct
     3361 ttttttatct atctcattta tagatatact tcctatcatt tcctattcct cctataagta
     3421 tatttactat caaccaaata taattggacc tattatacta gcaaatgcca ctgatgcaat
     3481 ggttacagta acaaatcctc ctataatcat ctttggtaat aaataatctg ttgccctttg
     3541 tttttcttct tcagttcctt ccatagcatc aataccttcg ttggaaatta tctgtgttgc
     3601 aggataagct agcatacaag ttacaccaat tgcagctgac attcttggag aatatcccaa
     3661 tagttttcct acaattgcag taaataatat tataaataca gagcctacaa caagtattcc
     3721 tatagttgga acaatcatcc caaacaatgc tgttggagtt accttagcaa gtcctccagg
     3781 taacatcaac atacaaccaa acattataat tccattagat tgagatttat ctaaaattcc
     3841 tttttctaaa aaacctattc gtgtaaaaat caaccctact aacaaacatg ctatattagg
     3901 atttaaataa tagtttgcag gggtaaatcc tggtattaaa gtagccaatc ctacaaaatt
     3961 accaatcata gcaacaaatg aaagctttgc aagatacact agagtagatg attttttacc
     4021 taaatcttct ttaaaaattc tcatacttgg aagtttaaaa ttactttttg actcatcttt
     4081 caaaaaatcc ccattatctc ttttaaattt taattcttta tttatacaat atgtagttat
     4141 cggaatgcca aagaactttt gaaatgataa tactagaatt acaaatgcag caagttcagg
     4201 tctatttgca gcttctgcaa cagttgttac gataatacca gcaacagtag aacccgctat
     4261 tggaggtgct gctattaaag catactctct accaaataac atactaccca ctgttaaaca
     4321 gccaacccct attcctacaa tacctactaa tgcaattaca accgttttcc attcatgaca
     4381 taagtcttca agatttataa gtgttcctaa gttagttatt aataaagcta taccaaagtt
     4441 gctcattgca gctaatatcc ctgatgctcc tgtaatgtct tttggtaaaa tatttgtcca
     4501 aaatcctatt aaaaatataa tacatacaaa taacaatgtt gaaattgcac cttttgtttt
     4561 cttagataaa aactcactaa aagcataaat cattacacat acagtaaagc aagtaagtgg
     4621 attccaaaaa ttttccattg ttgtttcctc ctaaatataa taatttttta tcaataaaac
     4681 aataattata aaatgtatat tatgcagtta ttcttaacta catatggtat agcttgatat
     4741 tttttgatga tattatatgc gtcctaaatt attcctaaaa tatctaaata aatataacta
     4801 gagttaactc tctaaatatt aactatagtt atcaacttac tagatttctt tttgattttt
     4861 actttctaca taattttacg agttagaatt gtacttcaac tactctctat attagtatac
     4921 atgttggaag acttatattt atttaaatat tagatataga atacattttt gcaaacgtct
     4981 taaattattc ttaaaatatc taaaacgaaa taataatata cccattaaac caatattatc
     5041 agaatatact tcactttaag tcttctttat atagaaattt tatctattag tgcatcccct
     5101 cctttgtaaa ttagactatt tctaaacgaa tttagatatc ttattcctag gaactattac
     5161 tagaaacaaa aattatttat tatattaaat caatgataag ccatacttta aaattctttc
     5221 tatacaagta tgatttatca tataatgaca tctatacc
//
LOCUS       DAELLV010000233         5251 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.233, whole genome shotgun sequence.
ACCESSION   DAELLV010000233 DAELLV010000000
VERSION     DAELLV010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.233"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(393..1799)
                     /locus_tag="KPD58_003168"
     CDS             complement(393..1799)
                     /locus_tag="KPD58_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0266899.1"
                     /translation="MDSVKYILRRFIGSSILISLLFIVLNIAGFLLFSYMLFVSKYSD
                     NLDTSDSREIKSNLIMIEDNLILRNGKFILDDSSKEVLHKNKVWAMLIDNNGDRIWGF
                     DLPKEIPSHYTLTDVAKFSRFFLKDYPVYVWEHPNGILVTGYPKDKYTKFNLTYTVKE
                     IEQFPMLIIGMFLVNAIVTFLIALFIGLKMVKSIKPIITGIKLMSKGEPVLLQEKGIL
                     SDISKSINTVSKELQMKDEKLRKKEEARSNWIAGISHDIRTPLSMIIGYAGELEESSS
                     LSNREQEQVSIICNQGIKIRELVNDLNLVSKLEYNMQTLNCDKIRVSAFLRELISEFI
                     NNNLDNDFYVELDILNEDIIIDADKKLLKRAISNLIQNSITHNQQGCNIRVTSKSDLD
                     FCYIIVEDDGKGMSQERINSLLKGQNGLSEAYAKGQNHGLGILIVSGIINAHRGKLDI
                     TSIDSQGLKTILKLPLIK"
     gene            complement(1844..2551)
                     /locus_tag="KPD58_003169"
     CDS             complement(1844..2551)
                     /locus_tag="KPD58_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0266900.1"
                     /translation="MESIKDKKILVVDDHKELLKMIDEILRKEGFSRVFLASSYEEAV
                     RVFRNVKPDCAILDVVLPDGDGFSIMRKIRETSKIPVIFLSARGEDEDRLIGLGLGAD
                     DYIVKPFLPKELTLRLVGILNRVYVPIEEEELPVFKLGDSVVVNLNSACVEKDGQEIS
                     LTAKEHSLLLKLYENKGRIVTNDSLCQAIWGDDMYGYENTLMVHVRRVREKIEKTPST
                     PKHLITVRGLGYKLMIN"
     gene            2722..3636
                     /locus_tag="KPD58_003170"
     CDS             2722..3636
                     /locus_tag="KPD58_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0266901.1"
                     /translation="MEMIIRTQRLSKEYNKIFRVKDIDLRVPQGAVYGFLGPNGAGKS
                     TTLKMLLGLAKPTQGNINILGKELNEKNRISILKDIGSLIESPSYYGHLTGLENMTIM
                     QRLLDLPEKNVNEALKIVRLENQKNKKVSQYSLGMKQRLGIAMAIMKFPKLLILDEPT
                     NGLDPAGIEEIRELIKSLPEKYGMTVLISSHLLSEIDQIATSIGIINHGELVFQDSIK
                     ELHNRGQSQIAIKTKNVVSAQKLLNTKGYMSLIQDGYLTIENLKDSDVAKINGMLVHS
                     NIDVFRIEEHKKSLEDIFLELTGKAVTL"
     gene            3636..4397
                     /locus_tag="KPD58_003171"
     CDS             3636..4397
                     /locus_tag="KPD58_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0266902.1"
                     /translation="MKYLGLEFYKLKRKKIILMTFLFVAVEIMWCIINSNRAITRNPD
                     MLGGFEYSYMLMSFASLNGLFFPILISIITSRISDIEHKGDTWKLLKSSVTSLNSIYL
                     SKFLCSAILVSIPVLMQVLSIVLFGHFRGVIESISVSLLLKYTLGTILVSIAIIALHL
                     WIATVIPNQMIAITFGMIGSFIGLTSGLFYQGIRRLFIWSYYLELSPLSYAYDNVLGS
                     SVYRIDMNFSITILVFLIGMLIYYIGRNHLLKKEV"
     gene            4409..5146
                     /locus_tag="KPD58_003172"
     CDS             4409..5146
                     /locus_tag="KPD58_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0266903.1"
                     /translation="MLRKSVLGEILKLKNSFIWYVLLALPLISVLIGSGNFYLNQGIL
                     KKEWYSLWTQVSLFYGEFFFPILIAIFCAYICRLEHMNHNWNNVMTLPVNLKNIFLSK
                     LTVVSILTAITQVFFVIFYIVAGNMFGFSGSVPKELTGWIAKGWIISISIASIQLYMS
                     IKIRSFATPIGISLCLSLFGMGLIVAKPAVGIFFPYSMLGSAMGVIKQSALNVNDTIV
                     TICVAFITTIVFSEIANRNFRKNDVIS"
BASE COUNT         1800 a          810 c          694 g         1947 t
ORIGIN      
        1 tacacagaac agcgtataat taaaactttt ttaggatgtc attatatttt ttcttaaaat
       61 taaaaaacac tataccataa ttctatttta gtagaatttt tttgtcaata ataaaattaa
      121 atattaacta aaaaataaaa aaaatggaat tatctaaaga atttaataac cttacaataa
      181 ttccaaaata ttatttctat gaaaattttg ataccatgag taactcagcc ataagcgcat
      241 aaagcacaag ttactcttta ctttaataaa acttgacaag aaaagaattt atccttattc
      301 tactctaata gagttaattt gtcaataaag aataaaatta ttttcaaaat attttcattc
      361 ttcattctta tctgttatta ttttataaaa tatcacttaa ttagaggtaa ttttagtatt
      421 gtttttagtc cctgactatc aatgctcgta atatctaatt tacctctatg tgcattaata
      481 atgccagata ctatcaaaat acctagacca tgattttgac ctttggcata tgcctcactt
      541 agtccatttt ggccctttaa taaactatta attctttctt gactcatacc ttttccatca
      601 tcttcaacaa ttatatagca aaaatctaaa tcagacttag aggtaaccct tatattacat
      661 ccttgttggt tatgagttat actattttgt attaaattac taatagccct ttttaataat
      721 tttttatctg catctataat aatatcttca tttagtatat caagttcaac gtaaaagtca
      781 ttatccaaat tattatttat aaactcagaa ataagctctc tcaaaaacgc agaaactcta
      841 atcttatcac aattcaatgt ttgcatatta tattcaagtt tagacactaa attgaggtca
      901 tttactaatt ctctgatttt aatcccttga ttgcatataa tagacacttg ctcttgttct
      961 ctatttgaaa ggctactgct ttcttcaagt tctccagcat atccaattat cattgaaagt
     1021 ggagttctta tatcatgaga tattcctgca atccagttag acctagcttc ctctttcttc
     1081 ctaagttttt catctttcat ttgcaactct ttagatacag tattaataga ttttgaaata
     1141 tcacttagta ttcctttttc ttgtaaaagt actggttctc cttttgacat aagctttatg
     1201 ccagtaataa ttggttttat agatttcacc atttttaaac ctatgaatag agctattaaa
     1261 aaagtaacta ttgcattaac cagaaacata cctattatta acataggaaa ttgttctatt
     1321 tccttaaccg tataagtaag gttaaactta gtgtacttgt cttttggata acctgttact
     1381 aatattccat ttggatgttc ccatacatac acaggatagt cctttaaaaa gaaccttgaa
     1441 aactttgcta catctgtcaa agtataatga cttggaattt cttttggtaa atcaaatccc
     1501 catattctat ctccattgtt atcaattaac attgcccaaa ctttattttt atgtagtact
     1561 tccttactag aatcatctaa aataaacttt ccatttctta aaatcaaatt atcttcaatc
     1621 ataatcaagt ttgattttat ttctctgcta tcacttgtat ctaaattatc agagtattta
     1681 gatacaaata gcatatatga aaacagaaga aaacctgcta tatttaatac aataaaaagt
     1741 aatgaaatca agatacttga acctataaat cgcctcaata tgtactttac actatccaaa
     1801 attaaatcac tcctcttgat ttcttttatt ctaatatcta aaattaatta atcatcaact
     1861 tatatccaag acctcttact gtaataagat gtttaggtgt agatggagtt ttttctatct
     1921 tctctctaac tcttctaaca tgaaccataa gggtattttc atatccatac atatcatctc
     1981 cccaaatggc ttgacatagt gaatcattag taacaattct ccctttattt tcatacaact
     2041 ttaaaagcaa cgaatgctct ttagctgtta aagaaatctc ttgcccatct ttctcaacac
     2101 atgcactatt taaattcaca acaacagaat ctcccagttt aaatactggc aactcctctt
     2161 cttctatagg cacatataca cgatttaata tacccacaag ccttaaagta agttcctttg
     2221 gtaaaaatgg ctttactata taatcgtcag cccctaagcc caaaccaatc aatctatctt
     2281 catcttcacc tcttgcagag agaaaaatca ctggtatttt agatgtctct ctaatttttc
     2341 tcataataga aaaaccatct ccatctggca atacaacatc taaaattgca caatctggct
     2401 ttacatttct aaatacccta acagcttcct cataacttga tgctaaaaat actctactaa
     2461 acccctcttt tctcaggatt tcatctatca tttttaataa ttccttgtgg tcatcaacca
     2521 caagtatttt tttatctttt attgactcca taatcatcac cctcctttag tataccatat
     2581 acccattttc acaactttgc ctaaatagac ttttaaggtt attgtaaggt aaatttcagc
     2641 taattgaaat gtaaccaagt tattatactg atataagtta aaagaatcaa taagcacaga
     2701 ttaaaaaagg ggatgaaaca tatggaaatg ataattagaa cacaaagact ttcaaaggaa
     2761 tacaataaaa tatttagagt aaaggatata gacctaagag ttccacaagg agctgtatat
     2821 ggttttttgg gtcctaatgg agctggaaaa tctaccactc ttaaaatgct tcttggactt
     2881 gctaaaccca cacaaggaaa tattaatatt ttaggtaaag agttaaatga aaaaaataga
     2941 atatcaattt taaaagatat aggttctttg atagaatctc catcatacta tggtcatctt
     3001 acagggcttg aaaacatgac cataatgcaa agactcttag atttacctga aaaaaatgta
     3061 aatgaagcat taaaaattgt aaggttagaa aatcaaaaaa ataaaaaagt tagtcaatat
     3121 tctttaggta tgaagcaacg tcttggcatt gctatggcaa taatgaagtt tcccaaatta
     3181 ttaatacttg atgaacctac aaatggactc gacccagcag ggattgagga aattagagaa
     3241 ttaataaaat ctctaccaga aaaatacggg atgactgttc taatctctag ccatctttta
     3301 agtgaaattg accaaattgc cacatcaata ggaataataa atcatggcga actggttttt
     3361 caagatagca taaaagaact tcataataga ggtcaaagtc agattgctat caaaactaag
     3421 aatgttgtat ctgcacaaaa actattaaat actaaaggat atatgtcact tatacaagat
     3481 gggtacttga ctattgaaaa tttaaaagac tcagatgtgg ctaaaataaa tggtatgctt
     3541 gtccattcaa atatagatgt atttagaata gaagaacata aaaaaagctt ggaagatata
     3601 ttcttagaac ttactggtaa ggcggtgact ttataatgaa ataccttggt ctagaatttt
     3661 ataaacttaa aagaaagaag attattctga tgaccttttt atttgtagct gttgaaataa
     3721 tgtggtgtat cataaattca aatagagcta ttacacgtaa tccagatatg ttaggtggat
     3781 ttgaatattc ctatatgctt atgagttttg caagtttaaa tggtctattc tttcctatct
     3841 taatatcaat aattacatca cgtataagcg atatagaaca taaaggagat acatggaagc
     3901 ttttaaaatc ttctgtgaca tctttaaata gtatttattt atctaaattt ttatgttctg
     3961 ctattttggt atctatacct gtattgatgc aagtgctatc tattgtgttg tttggacatt
     4021 ttagaggggt tattgagtca atttctgtat ctttactttt aaaatacaca ttaggaacaa
     4081 ttcttgtaag tattgctatc attgcactac acctatggat tgcaactgta attccaaatc
     4141 aaatgattgc aattacattt ggcatgattg gtagttttat aggtttgaca tcaggtttgt
     4201 tttatcaagg tattcgtaga ttgtttatat ggtcttatta cctagagtta tcacctttaa
     4261 gttatgctta tgataatgtt ttaggttcat ctgtctatag aattgatatg aatttttcta
     4321 ttacgatttt agttttctta attggaatgc taatttatta tattggaaga aatcatttat
     4381 taaaaaaaga agtataggag ggatttaaat gttaaggaag tcagttttgg gagaaattct
     4441 aaagttaaaa aattctttta tatggtatgt attattagct ttgccactta taagtgtgtt
     4501 gattggttct ggtaattttt atctaaatca aggcatactc aaaaaagaat ggtacagctt
     4561 atggactcaa gttagtcttt tttatgggga atttttcttt cctatcttaa tcgcaatctt
     4621 ttgtgcatat atttgtaggc ttgaacatat gaatcataat tggaataatg taatgacact
     4681 gccagttaat ttaaaaaata tctttttatc taaacttaca gtagtttcaa tacttacagc
     4741 tattacacaa gttttctttg ttatctttta tatagttgca ggaaatatgt ttggtttttc
     4801 tggtagtgta cctaaagaat tgactggttg gattgctaaa ggttggatta tttctatttc
     4861 aatagcttct atacaattat acatgtctat aaagattaga agttttgcaa ctcctattgg
     4921 tattagtctt tgcctttctc tatttggtat gggattaata gtagcaaaac ctgctgttgg
     4981 tatatttttc ccttattcta tgttaggtag tgctatgggg gttataaagc aatctgcttt
     5041 aaatgttaat gacactatag ttacaatatg tgttgcattt attactacta tagtattttc
     5101 tgaaattgcc aatagaaatt ttagaaagaa tgatgttatt tcttaatggt aaatgctaga
     5161 tattttgggg tttgaaattt atattctttt tagttgaaat ctatatccat tttatttaaa
     5221 aatatatatc ttttatttga attttatatg t
//
LOCUS       DAELLV010000234         5211 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.234, whole genome shotgun sequence.
ACCESSION   DAELLV010000234 DAELLV010000000
VERSION     DAELLV010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5211
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.234"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..745
                     /locus_tag="KPD58_003173"
     CDS             <1..745
                     /locus_tag="KPD58_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBG0266904.1"
                     /translation="KILISKKNNVILGGCGFIKLSNKNIGTAIDLKDINLNYIKEDKK
                     NILIGADTSLRSLEIDKTIKNYCSGILSSAVSNIVGVQFRQGARIGASVFSKYGFSDL
                     IPALLVVGAKVRLYNKGILELSEFLDSELSRDILVEVILPKKDAIAVFDSIRKCTGDF
                     SVLNSAMLKENDTYKIAIGARPQKARLALEASNILNKEKDIDKASIVASKELTYGSNM
                     RASKEYRKDMASALVKRMYNAIEEGMYND"
     gene            738..3044
                     /locus_tag="KPD58_003174"
     CDS             738..3044
                     /locus_tag="KPD58_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG0266905.1"
                     /translation="MINKGIRKIDSTAIVTGKPLYTEDLIMHKDVLTLKLLRSPHAHA
                     KIKNIDTSKAEKIPGVVGIYTYKDVPQVRYCVPGQTEPEASPYDRVLLEDTVRFIGDE
                     VALVAAEDEKTALKALKMIKVDYEVLKPVFDAREAEGNEVVIHPEKDIVEPYPFGLDA
                     KNNIVSKEFFEFGDVEAKLKESDFVVESSYKTQSQSHCMMETHRSYSYKDINDRIVVV
                     SSLQSPHNVKRILRKVLGIPSSKIRVIKPRIGGGFGGKNIAATDIFTAFVTMKTGRPA
                     KFVFTRSETFVGSNSRHEMFFDVKVGADKEGNLKAIELKSLNNTGAYGGNGVSVSSES
                     GHNTLPIYNDIDAVRFNARTVYTNRLPAGALRGYGATQGIFAIDSAIDELAHKIGMDP
                     LEFRIKNIIDRYTNGKAAEDEILSCELRQCIEKGKKLINWDDKYPCKDIGNNKVRAVG
                     MAVANHGSGIPRVDMATVTIRMDEDGTYKLLSGLADLGQGADTIQAQIAAEALNTTVD
                     RISIYTGDSDLCPYDTGAFASCTTTVTGNATIKTANKLKDILLSVAESKLGFKKEDLE
                     LGKDRVFVKNDNSKFVLLEELGRESVGGIGFASDDVPVVLEATESFGMLKKIKPFIAG
                     FAEIELDKNTGEYKVINFACVPDCGTVINPTLARIQVEGGVMMGIGFAMYEDPIFDKD
                     GRLLTDSFLQYKVPTKKEVGNILVDFAENFDPGGPYGAKSLGEIVIHTPAPAIANAIF
                     NATGVRLRELPMTFEKVYMGMKNNNKQL"
     gene            3183..4460
                     /locus_tag="KPD58_003175"
     CDS             3183..4460
                     /locus_tag="KPD58_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG0266906.1"
                     /translation="MSNRIKNIHYGTQRQQLKNLTKKEYQALREMCFLAKNLYNVGTY
                     NVRQHYFNQGEHLNYEANYHVCKENENYKLLNSNIAQQILKEVDSSFRSFFGLIKLAK
                     EGKYDFRGIKSPKYLDKEGFFSIIIGQIRIKEDGILNVPMSPAFKKLYGKVSIKVPQN
                     ILEKKIKEIRIIPKHKARFFEIQYCYEIPKSEEVSNQKNALAIDLGLENLCTCVTNLG
                     DSFIIDGKRLKSVNQWANKQNDKIEALKLKKNITAITRKQFKVWNKRNNQVKDYVNKT
                     CFYIIDYCVKNNIGNLIVGYNAKLEKSDNMERKIYRNFIRIPFGEIKGKLEYLCKIKN
                     INFIRQEESYTSKSDFFADDFLPDINVDNVKKYDFKGERITRGQYKSSVGIIINADVN
                     ASLNILKKSQVVNLSNLQNNPQILKQPQRVRIS"
     gene            4643..>5211
                     /gene="yqeC"
                     /locus_tag="KPD58_003176"
     CDS             4643..>5211
                     /gene="yqeC"
                     /locus_tag="KPD58_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HBG0266907.1"
                     /translation="MELSNIIEKNEIITVVGAGGKTSLINYFANFYRDKLKVLLTTTT
                     KIYVPNDYDNIIITIDGTVIPSICHGITVCGSYINNENKLVSIDSSILDEIVDQFDLV
                     LIEGDGSKRKKLKGWNAKEPVVYHKTTKTIGILDITSFGMNINEENIHRVEIFKKIAN
                     LDTSSINSSSTVSIENLKNIVLNPNGLFK"
BASE COUNT         2120 a          565 c          986 g         1540 t
ORIGIN      
        1 taaaatttta atttctaaaa aaaataatgt tattttaggt ggatgtggat ttataaaatt
       61 aagtaataaa aatataggaa cagcgataga tttaaaagat ataaatctaa attatataaa
      121 agaggataaa aaaaatattt tgataggtgc agatacttca cttagaagtt tagaaattga
      181 taaaactata aaaaattact gtagtggaat actttcaagc gctgtttcta atatagttgg
      241 tgttcaattt agacaagggg ctagaattgg tgcaagtgta ttttcaaagt atggcttttc
      301 tgacttaata ccagcgcttt tggttgttgg agcgaaggtg agattataca ataaaggaat
      361 actagagtta agtgagtttt tggatagtga attaagtagg gatatattgg tcgaagtaat
      421 actaccaaaa aaagatgcaa tagctgtttt tgactcaata agaaaatgca caggagattt
      481 ttctgtatta aattcagcaa tgctaaaaga aaatgatact tacaaaatag caattggggc
      541 aagacctcaa aaagctagat tagcacttga agcgagtaat atattaaata aagaaaaaga
      601 tatagataag gcatctattg ttgctagtaa ggaacttact tatggaagta atatgagagc
      661 tagtaaagag tacagaaagg atatggcaag tgcattagtt aaaaggatgt ataatgctat
      721 tgaggaggga atgtataatg attaataagg ggataagaaa gatagatagt acagcaattg
      781 tcacaggaaa acctctatat actgaagacc tcattatgca taaagatgtt ttgacattaa
      841 aactacttag aagtccacat gctcatgcta agataaagaa tatagataca tcaaaagccg
      901 aaaaaattcc aggggttgtg ggtatttata cgtataaaga tgtaccacag gtcagatatt
      961 gtgtgccagg tcaaacagaa ccagaagcat caccttatga tagagtttta cttgaagata
     1021 cagttagatt tattggagat gaagtagctt tagtggctgc tgaagatgag aaaacggcac
     1081 ttaaagcctt aaaaatgata aaggtagatt atgaggtttt aaagcctgtg tttgatgcaa
     1141 gagaagcaga aggaaatgaa gttgttatac atccagaaaa agacatagta gagccatatc
     1201 cttttggatt ggatgcaaaa aataatatag tatcaaaaga attttttgaa tttggagatg
     1261 ttgaagctaa gcttaaagaa agtgattttg tggttgaatc ttcatacaaa acccaaagtc
     1321 aatcgcattg catgatggaa actcataggt catattcata taaagatata aatgatagaa
     1381 ttgttgtagt ttcatcatta caatcaccac acaatgtaaa gagaatactt agaaaagtat
     1441 tgggtatacc atcttcaaaa ataagagtaa ttaaacctag aataggtggt ggatttggag
     1501 gaaagaatat agcagcaaca gatatattta ctgcatttgt tactatgaaa actggtagac
     1561 cagcaaaatt tgtatttaca agaagtgaaa cttttgtagg ttctaatagt agacatgaaa
     1621 tgttttttga tgttaaagtt ggtgcagata aagaaggaaa tttaaaagca atagagctaa
     1681 agtcattaaa caatacaggt gcttatggtg ggaatggtgt ctctgtttct tccgaatctg
     1741 gacacaatac cttaccaata tataatgata ttgatgctgt tagatttaat gctagaacag
     1801 tatatactaa taggttacca gcaggagcac ttagagggta tggtgctaca cagggaatat
     1861 ttgcaataga tagtgctatt gatgaattgg cacataagat tggaatggat ccacttgaat
     1921 ttagaattaa aaatataata gataggtata ccaatggaaa agcagctgaa gacgaaatat
     1981 taagttgtga gctgagacaa tgtatagaaa agggcaagaa attaattaat tgggatgata
     2041 aatatccatg taaagatata ggaaacaaca aagtaagagc agtaggtatg gcagtagcaa
     2101 atcatggttc tggtataccg cgtgtagata tggcaacagt cactataaga atggatgaag
     2161 atggcacata taaactatta tctggtcttg ctgacttagg tcaaggggct gatacaattc
     2221 aagctcaaat tgctgcggaa gctttaaata ctactgtaga tagaatttct atttatacag
     2281 gtgattcaga cttatgtccc tatgatacag gagcatttgc ttcttgtacg acaacggtta
     2341 caggaaatgc aacaataaag acagcaaata aattaaaaga tatattactt tcagttgcag
     2401 agtcaaagct aggatttaaa aaagaagatt tagagttggg aaaagacagg gtttttgtta
     2461 aaaacgataa tagcaagttt gttctattag aggaattggg tagagaatct gttggaggaa
     2521 taggatttgc tagtgatgat gtaccagtag ttttggaagc aacagaatct tttggcatgt
     2581 tgaaaaaaat aaaaccattt atagcaggat ttgcagagat agaattagat aaaaatacag
     2641 gcgaatataa agtcataaat tttgcatgtg tacctgattg tggaacagtt ataaatccaa
     2701 ctttagctag aatacaagta gaaggtggtg tgatgatggg tatagggttt gcaatgtatg
     2761 aagaccctat atttgataaa gatggtagat tattaacaga tagtttccta cagtataaag
     2821 ttcctacaaa aaaagaagtt ggaaatatat tggtagattt tgctgagaat ttcgacccag
     2881 gaggtcctta tggtgctaaa tcattaggag aaatagttat acatacacca gcaccagcta
     2941 tagcaaatgc aatatttaat gctacaggag ttcgtttaag agagcttcct atgacttttg
     3001 aaaaagttta tatgggtatg aaaaataata ataaacaatt ataataaata ataagatgta
     3061 taaaagcaag taaatttaaa ttattttgta aaataaagta attaaatgga cattatatat
     3121 ggtgttttgt aaaaataccg tatataatgt ctagtagagt atattctaaa ggaggtgagt
     3181 tcatgtcaaa cagaataaaa aatatacact atggaactca gagacaacaa ttaaaaaact
     3241 taactaaaaa agaatatcaa gctctacgag aaatgtgctt cttagctaag aacttatata
     3301 atgttggtac atataatgta agacagcatt atttcaatca aggagaacat cttaactatg
     3361 aagctaatta tcatgtatgc aaagaaaatg agaactataa gcttctcaat agtaatatag
     3421 cgcaacaaat attaaaagaa gtagacagtt cgtttaggtc attttttgga ttaattaaat
     3481 tagccaaaga aggaaaatat gactttagag ggataaaatc acctaaatat ttagataaag
     3541 aaggattttt tagcataata ataggtcaaa taagaatcaa agaagatggg attttaaatg
     3601 tacctatgtc tccagcattt aaaaaactat atgggaaagt aagtataaaa gttccacaaa
     3661 atatactaga gaaaaaaatt aaagaaataa gaataatacc taaacataaa gctaggttct
     3721 ttgaaattca gtattgttat gaaataccaa aatcagagga agtttcaaat caaaaaaatg
     3781 cactagctat agacttgggt ttagaaaatt tatgtacatg tgttaccaat ttaggtgata
     3841 gttttatcat agatggaaaa aggctaaaat ctgtgaatca gtgggcaaac aaacagaatg
     3901 ataaaataga agccttaaaa ttaaaaaaga atataactgc aataacaaga aaacaattta
     3961 aagtttggaa taagagaaat aatcaagtta aagactatgt aaataaaacc tgtttttata
     4021 taattgatta ttgtgtaaaa aataatattg gaaatttaat tgtaggatat aatgctaagt
     4081 tagaaaaaag tgataatatg gagagaaaaa tatatagaaa ttttataaga atcccatttg
     4141 gagagataaa aggaaaatta gaatatctgt gtaaaataaa aaatatcaat tttattaggc
     4201 aagaagaaag ctatactagt aaatcagatt tttttgctga tgattttcta cctgatatca
     4261 atgtggacaa tgttaaaaaa tatgacttta aaggggaaag aataacgcgt ggacagtaca
     4321 aatccagtgt tggaatcatt ataaatgcag atgtaaatgc gagtcttaat atactaaaaa
     4381 aatcacaagt agttaattta tctaatttac agaataatcc tcaaatatta aagcaaccac
     4441 agagagtaag aatatcttaa ttatatttaa aagataaact gatagcgtaa gcttacagct
     4501 tagcggcgaa ttggatccgc cttgttgttt acaacagaat ctatttactt tcgagtttat
     4561 ggaatttcaa aaatcacttt tactaaaaag gtctaactat aataattaag agattagaaa
     4621 actggttata agaggtgaaa gtgtggaact atcaaatata atagaaaaga atgaaataat
     4681 aactgtagtt ggagcaggag gaaaaacttc gcttataaac tattttgcaa atttttatag
     4741 agataaattg aaagttctcc ttacaacaac aactaaaata tatgtaccaa acgattatga
     4801 caatataatt attacaatag atggaactgt tattccatct atttgtcatg gaataacagt
     4861 ctgtggcagt tatataaata atgaaaataa attagtaagt attgattcct ctattttgga
     4921 tgaaattgta gaccaatttg atttagtttt aatagaggga gatggctcaa agagaaaaaa
     4981 acttaaaggt tggaatgcta aagaaccagt agtatatcat aaaactacaa aaaccatagg
     5041 gatattagat ataacatcat ttggaatgaa tattaatgaa gagaatatac atagagtaga
     5101 aatatttaaa aaaatagcaa atttagatac aagtagtatt aatagtagtt ctacagtaag
     5161 tattgaaaac ttaaaaaata tagtattaaa tccaaatgga ttatttaaaa a
//
LOCUS       DAELLV010000235         5211 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.235, whole genome shotgun sequence.
ACCESSION   DAELLV010000235 DAELLV010000000
VERSION     DAELLV010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5211
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.235"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            218..1699
                     /locus_tag="KPD58_003177"
     CDS             218..1699
                     /locus_tag="KPD58_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBG0266908.1"
                     /translation="MKKNRLIAKNISIAFIVLFFFSVFTFFYVGNINRVLEYETNDII
                     TVTIAGWIILSFLFLGIIIYILYSKANSQKTIEKVAYTDFVTGYSNWRKFELDVTNLL
                     KKTSQNNKYAMVIFDIDKFKAINDIYGHKKGNLILKDIADTLNELTNINETFARVSAD
                     SFNILLTYNKKEDIINIIKKIMANNELVNLSFGIYEIKDKDLSVSVYSDRASLAKSSI
                     KNNSDVNFAFFNDKLREKLLLEDKIEKEMEYALESGQFVMYLQPKYNIKLDKFCGSEA
                     LVRWQYTEKEVIYPGDFIPIFEKNGFIRKIDMYILEQACKEIRSLFDKGISPLPISVN
                     FSRVDFFKKDFIENIVNICDRYKIPYSLIEIEITESSMFGDTDTLFNVSRNLQDIGFI
                     VAMDDFGSGYSSVNMLKNIPLNVIKLDRGFFVDDKDVDKSQIVIKSIVSLIKQLGIRV
                     VAEGIETRSQIEMLKKANCDIVQGYYFSKPLPIKEFEKLVYKI"
     gene            1732..5169
                     /locus_tag="KPD58_003178"
     CDS             1732..5169
                     /locus_tag="KPD58_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBG0266909.1"
                     /translation="MIRAKSLKNVLSIVLIFLFTIISFSDFAFKTNVQEEENSKINLR
                     EISLQSSNAIKIKLDSSLSFIKSVANMVSYFNTIDNSKIVPLLANISKESDFQRISIV
                     SPDGISHTSDGKIINVSKYQYFKKAIKGESNISEVVKSPIDGKDTFVIATPVFNKNNK
                     IIGVLYGSYYNDKLAEFIDITSFNSEGYIDIFESSGQFVLKSSHTNKLIKSSSNIYDI
                     FKKASFDDGYSYKKIYEDTKNNKSGFTSYEYKGENRYATYTPIGVNDWYVISVIPQYT
                     VRRNIDKINQLAFSLIFKVFLLFIMLIIYIIYKNNKSQKDILKANKEISSITNSIPGG
                     VQKCTLGETCNINFLSDGFIELFGYTREEIKTLFNNDFYSLIHKDDVERIKKEIFKQL
                     YKNKTIELEYRIITKNGNTVWVLDKGELITEPDGKEYFCCVTVDITRTKQLEQEMEQK
                     NLELEMISENVLGGIIITEFNDDFTIKYANSGYLSMIGYTREQLENELKYKACNLVFY
                     EDKKKALESIQHQLNTKGIAVYQHRIRRRNGNIIWVSIKGRLIVDDDGIEKGIWILND
                     ITDLKNAEQKLKINEERFRIALKQTSNTIFEYDIKTKSLLNTSKELDKYNIYKITKNV
                     PYSLVESGVIHKDFSDDFINMYENVINGAKKASCVVKMKLPDENYIWNKVTLTTIFDE
                     DGNPVRAIGIQEDISKQKEAEIRYNQEGKYHSAILSEAMITYEINVSKDTFIKGNESW
                     QKPFGIKPDNNYSQMVKTIANSIVHKDNKETFLNTFLCENLLDAYKNNVTEIKLEYRR
                     MNQENKMIWVICTMHLIKDSITNDIKALAYVKDIDKQKNEEIRLKLKAERDTLTGLYN
                     KGTTEKLINDFLNSEEASLGMHAFLIVDIDNFKSVNDNLGHVFGDKVLFEVSKNLKPI
                     FRKDDIVGRIGGDEFIVFLKNIQSFENIKKKAEKICDIFRNSYTENKKTYKISGTVGI
                     SFVPEHGTVFEELYKKADIALYYAKSKGKDKYALYNDCIYQNELENNISDNNIKLADK
                     CEKSKPVLKEILDSIDNYIYIVNPNTYELLFINNKVSRLDLEIKLGETCYKSLLKSDR
                     PCNNCPLNGLEENKHSKFKIETNTFGKNISIASWINCIDGEYNCLIECIDSSLYKNK"
BASE COUNT         2193 a          499 c          799 g         1720 t
ORIGIN      
        1 tatttaattg tattaataaa tatggattca atataattaa atatgctatt gatatatttg
       61 tacccttaac tattgttatc ttttaaatga taatgatatt tatttaaata ctctttatta
      121 acaatttata aaacttaatt gtaaaaaata cttattaata gtaaaataaa gtgaatcaat
      181 tatattataa ttgctaaaga gagtgaggtg tagctatttg aaaaaaaata gattaatagc
      241 aaagaatata tcaatagcat ttatagtact attctttttt agtgtattta cattttttta
      301 tgtaggtaat attaatagag tgttagaata tgaaacaaat gacataataa cagttacaat
      361 tgctggatgg attatacttt catttttatt tttaggtatt ataatttaca tattatattc
      421 aaaagcaaat agtcaaaaaa ctatagaaaa agtagcatat acggattttg taacaggtta
      481 ttccaactgg agaaagtttg aattagatgt tacaaattta ttaaagaaaa catcacaaaa
      541 taataaatat gcaatggtca tattcgatat tgataaattt aaagcaatta atgatatata
      601 tggtcataaa aagggaaatt taatattaaa agatattgca gatactttaa atgaattgac
      661 taatatcaat gaaacttttg ctcgtgtaag tgctgatagt tttaatatat tattaacata
      721 taacaaaaaa gaggatataa taaatattat caaaaagatt atggcaaata atgaacttgt
      781 aaacttatct tttggaattt atgaaatcaa agataaggat ttatctgtaa gtgtatatag
      841 cgatagagct tcacttgcaa aatcatcaat aaaaaataat agtgatgtaa attttgcatt
      901 ttttaatgat aaattaagag agaaattatt acttgaagat aagatagaaa aagaaatgga
      961 atatgctctt gaaagtgggc aattcgtaat gtatttacaa ccaaaataca atattaaatt
     1021 agataaattt tgtggaagtg aagccctagt cagatggcaa tatacagaaa aagaggtcat
     1081 atatccaggt gattttattc ctatatttga aaaaaatgga tttattagaa agattgatat
     1141 gtacatatta gaacaagctt gtaaagaaat ccggtcttta tttgataaag gaatttcacc
     1201 tttacctata tctgtaaact tctcaagagt tgattttttt aaaaaagatt ttattgaaaa
     1261 catagtaaat atatgtgata gatataaaat accatatagt ttaatagaaa ttgaaattac
     1321 tgaatctagt atgtttggag atactgatac tctatttaat gttagtagaa acctacagga
     1381 tattggcttt atagttgcaa tggatgattt tggctcggga tattcttctg taaatatgct
     1441 taaaaatata ccattaaatg taataaaatt agatagagga ttttttgttg atgataaaga
     1501 tgttgataag agtcaaattg ttataaaaag catagtttct ctcattaagc aattaggaat
     1561 aagggttgtt gctgaaggaa ttgaaacaag aagtcaaata gaaatgttaa aaaaagcaaa
     1621 ttgtgatatt gttcaaggat attatttttc aaaaccatta ccaattaaag aatttgaaaa
     1681 attagtctat aaaatatagt cagcgagtct taaatgggag gtggtgatta agtgataaga
     1741 gcaaaatcgt taaaaaatgt actgagtatt gttttaatat ttttatttac aataatttct
     1801 ttttctgatt ttgcatttaa aactaatgtc caggaagaag aaaatagtaa aattaattta
     1861 agggaaatat ccctacaaag ttccaatgct ataaaaataa aattagatag ttctctatcc
     1921 tttattaaaa gtgtggcaaa tatggtttct tattttaata ctatagataa ctcaaaaata
     1981 gttccattat tagctaatat atcaaaagaa tctgactttc aaagaatctc tattgtatct
     2041 cctgatggaa taagtcatac cagtgatggc aaaattatca atgtgtcaaa gtatcagtat
     2101 tttaagaaag ctattaaagg agaaagtaat atatctgaag ttgtgaaatc tccaattgat
     2161 ggaaaagata cttttgttat agctactcct gtatttaata agaataacaa aataattggt
     2221 gttttatacg gttcttacta caatgataaa ttagctgaat ttatagatat aacaagtttt
     2281 aatagtgaag ggtacataga tatatttgaa tctagtggtc aatttgtttt aaaatcttca
     2341 catacaaaca aactgattaa gtcaagtagt aacatatatg atatatttaa aaaagcttct
     2401 tttgatgatg gttatagcta taaaaaaata tatgaagata caaaaaataa caagagtggc
     2461 tttacctcat atgaatacaa aggagaaaat cgttatgcta cttatacacc cattggtgta
     2521 aatgactggt atgtaatttc tgtgatacca caatatactg taagaagaaa cattgataaa
     2581 ataaatcaat tagcattttc tcttattttc aaggtattct tattgtttat tatgcttata
     2641 atatatatta tttataaaaa caataaatct cagaaagata tactaaaagc taacaaagaa
     2701 atctcatcta ttacaaatag cattcctgga ggagtacaaa agtgtaccct gggtgaaaca
     2761 tgcaatatta attttttaag tgatggattt atagagttgt ttggttatac aagagaagaa
     2821 ataaaaacat tgtttaataa tgatttttat agtttaatac ataaagatga tgttgaaaga
     2881 ataaaaaaag aaatttttaa acaactttac aaaaataaaa ctattgaatt agaatatagg
     2941 attataacaa aaaatggtaa tacagtatgg gttttagata aaggggagct tataacagaa
     3001 cctgatggaa aagaatattt ttgttgtgta actgtagata ttactagaac aaaacaatta
     3061 gaacaagaga tggaacaaaa aaatctagaa cttgaaatga tatcagaaaa tgttttaggt
     3121 ggtataatta taacagaatt taatgatgat tttactataa aatacgctaa ctctggttat
     3181 ctttcaatga ttggatatac acgtgaacaa ttagaaaatg aacttaaata caaagcttgt
     3241 aatcttgttt tttatgagga taaaaagaag gctcttgaaa gtatacaaca tcaattaaac
     3301 acaaaaggaa tagctgtata tcaacataga ataagaagaa gaaatggtaa tattatttgg
     3361 gtttctataa aaggaaggct tattgttgat gatgatggta ttgaaaaagg tatttggatt
     3421 ctaaatgata taacagattt aaaaaatgca gaacaaaaac ttaaaataaa tgaagaaaga
     3481 tttagaattg cattaaagca aacatcaaac actatatttg agtatgatat aaaaacaaag
     3541 tcacttttaa atactagtaa agagcttgat aaatataata tttataaaat tactaaaaac
     3601 gttccatatt ctctagtaga atcaggagtt attcataaag attttagtga tgattttata
     3661 aacatgtatg aaaatgtaat aaatggagca aaaaaagcaa gttgtgtagt taagatgaaa
     3721 cttccagatg aaaattatat atggaataaa gttaccttga ctactatttt tgatgaagat
     3781 ggtaatcctg taagagcaat tggtatacag gaagatataa gcaaacaaaa ggaagcagag
     3841 ataagataca atcaagaagg taagtatcat tcagctatat tgtcggaagc aatgataact
     3901 tatgaaataa atgtatcaaa ggatactttt atcaaaggaa atgaaagttg gcaaaaacca
     3961 tttggaatta agcctgacaa taactattct caaatggtaa aaactatagc aaatagtatt
     4021 gtacataaag acaataaaga aacattttta aatacatttt tatgtgaaaa cttattggat
     4081 gcatataaaa ataatgtgac tgaaataaaa ttagaatatc gaagaatgaa tcaagaaaat
     4141 aaaatgattt gggtcatttg tacaatgcac ttaataaaag attctattac gaacgatata
     4201 aaagcacttg cttatgttaa agatattgac aagcaaaaaa atgaagaaat tagattaaaa
     4261 ttaaaagctg aaagagatac tcttacagga ttatataata aaggtactac agaaaaattg
     4321 attaatgatt tcttgaactc tgaggaagca agtttaggaa tgcatgcatt tttaattgtt
     4381 gatatagata attttaaatc tgtgaatgat aatttaggac atgtatttgg agataaagta
     4441 ttatttgaag tttctaaaaa cctaaaacct atatttagaa aagatgatat agtaggtaga
     4501 attggcggag atgaatttat agtattctta aaaaatattc agtcttttga gaatatcaaa
     4561 aagaaagcag aaaaaatatg cgatatattt agaaattctt atacagaaaa taaaaagaca
     4621 tataaaatat ctggaacagt tggaatttca tttgtaccag aacatggaac tgtatttgaa
     4681 gaactttata aaaaagcaga catagcactt tattatgcaa agagcaaagg aaaagataag
     4741 tatgcacttt ataatgattg tatctaccaa aatgaacttg aaaataatat aagtgataat
     4801 aatataaaat tagcagataa atgtgagaaa tccaaaccag tacttaaaga aattcttgat
     4861 tctattgata attatatcta tatagtgaat ccaaatactt atgaacttct ttttatcaat
     4921 aataaggtgt ctcgtttgga tttagagata aaattaggag aaacctgtta taaatcacta
     4981 ttaaaatctg atagaccatg taataattgc cctcttaacg gattagaaga aaataagcat
     5041 tcaaaattca aaatagagac aaatacattt ggtaaaaata tcagtatagc aagttggatt
     5101 aattgcatag atggagaata caactgtctt atagagtgta tagattcttc tttatacaaa
     5161 aataaataaa aaaggactat gaagatatgt taaatagtat aactttgata c
//
LOCUS       DAELLV010000236         5201 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.236, whole genome shotgun sequence.
ACCESSION   DAELLV010000236 DAELLV010000000
VERSION     DAELLV010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5201
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.236"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            186..1274
                     /locus_tag="KPD58_003179"
     CDS             186..1274
                     /locus_tag="KPD58_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HBG0266910.1"
                     /translation="MRDFSTYSKDDVIFLLKDISNLIEEEGNKERELKIQNGRHYSEM
                     IPIEYEVSEDYLNLYHEKLRENKDKLAFSIGVMCEKIIKKNGNDVVLVSLARAGTPIG
                     ILAKRYIRSKYNISLPHYTISIIRDKGIDMNAIEYIVKNHPSSKIQFLDGWTGKGTIS
                     KELEKACDELNVKFKKYFDSTLAVLADPAGYSGLYGIREDFLIPSACLNSTVSGLVSR
                     TVLREDLIGKNDFHGAKFYRHLKDKDESMNYIETIEECFKNQFKNISDEVENWESDII
                     TRDGYFDVLNIKEKYNITDINFIKPGVGETTRVLLRRVPYKILVKDLNDKSLDHIFIL
                     AKEKNVEVEQMDLKAYKCCGIIKNMKDI"
     gene            1302..2093
                     /locus_tag="KPD58_003180"
     CDS             1302..2093
                     /locus_tag="KPD58_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HBG0266911.1"
                     /translation="MIVFSDLDRSIIYSNKFLNADSKYTNIEIYREKEISYISLDTIN
                     LIKQIQYYGMFIPTTTRTVEQFKRIEFNKYGIYFPWSITSNGGVILKDNEILKSWSEK
                     IDKLKCDYEPIESMIHKFKDYLNVDGITNFKVAEDTFFYIVVDLSRFNLDSIKEYTNI
                     LESKNWKFYVSGRKIYFIPKEISKENAIKYLTKELGIEYFYAVGDSIMDYGMLNISNK
                     SYVLKHGDINKNEIENSFISSSFNGMSGTEEILSNILDENCLLNI"
     gene            complement(2208..2762)
                     /locus_tag="KPD58_003181"
     CDS             complement(2208..2762)
                     /locus_tag="KPD58_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBG0266912.1"
                     /translation="MNIDVGSKIRSLRKSKNISISTLAKNSDLSTGLISQIERNMVVP
                     SIVAMWKISKALDINIGYFFEEIGREDSDIVVKKNNRKKIVTNDSTKFYELLVPNLSG
                     KKIEFILVTLDGHTQKNSEFVTHEGEECGYIIKGKMKITLGNKEYLLEEGDSFYFNST
                     IPHVYENYDDEVCISVWAMTPPSF"
     gene            2958..4160
                     /locus_tag="KPD58_003182"
     CDS             2958..4160
                     /locus_tag="KPD58_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 domain-containing protein"
                     /protein_id="HBG0266913.1"
                     /translation="MKSFVSPDNIWVLWAVVTGWAAFSIYLEQKYNWASKVSGAIIAL
                     VGAMLLSNLNIIPVESVVYDQVWGYVVPLAIALLLYQCNIKKIWKESGRLLIIFLVGS
                     VGTVLGAMIGFLALKNVVPDLNIVAAMMTGSYIGGNVNFAAMSGAFDAPGELVSATVV
                     ADNLLMALYFFVLIAIPSIGFFRKHFKHPHVDEMESIGITEGETVAANYWGRKEISLK
                     DIAIAIGSAFIIVAVSVELSTWFKAIIPLSNPFLAMLNTLFGNQYLIITTLTMLFATF
                     MPNFFGDIKGAQELGTFLIYIFFVVVGVPASITLIIQKSPLLLLYCGIMVLINMLVTF
                     IVAKIFKFSLEEAILASNANIGGPTTAAAMAISKGWSKLVGPILIVGTFGYIVGNYFG
                     LLVGNILI"
     gene            4217..5197
                     /locus_tag="KPD58_003183"
     CDS             4217..5197
                     /locus_tag="KPD58_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HBG0266914.1"
                     /translation="MIFDGHSDTWADVTIRRLNGEKDVFRRIHLDNFKRANIEGAIFV
                     IWIDPPYDKEPEKRVKQIISSIKDEIEDMKDVVNIVKKYSDIDKGISENKINILIGLE
                     GLSHIGRNIDLLDYFYKEVGARHASLTWNEQNELATGCLGDENRGLSEIGKKAVNKME
                     KLGMIVDVSHLNEKSFWDLMSVASKPVIASHSNARSLCDAKRNLTDNQLKEISNTGGL
                     VGINSFRGFTNSNPKKQDLIHLVDHIDYMVNLIGIEHVSFGFDFSDYLGKETIGSFTG
                     NASVPSVDGLESTHTAQNIVVELENRGYKKKDIELISSGNFYRLFKDILK"
BASE COUNT         2002 a          550 c          880 g         1769 t
ORIGIN      
        1 atagataaac ataatttttc taagtgttat ttatttgtag agctgaataa aagtgaaaat
       61 tcttatctag aatttattga tataattaaa aaaacaagta taaaagaatt aactatagta
      121 tgttgtagct aaagtgtttt aacaggataa aatatatcac gattttaaga taggagttga
      181 gtcatatgag agattttagc acgtattcta aagatgatgt tattttttta ttaaaagata
      241 tatcaaattt aattgaagaa gaaggaaata aagaaagaga attaaaaata caaaatggta
      301 gacattattc agaaatgata ccaatagagt atgaagttag tgaagattat ctgaatttat
      361 atcatgagaa actaagagaa aataaagata aacttgcatt ttctatagga gttatgtgtg
      421 aaaaaataat taaaaaaaat ggaaatgatg tagtactagt ttcacttgct agagcaggaa
      481 cacctattgg tattttggca aaaagatata taagaagcaa gtataatata agtttacctc
      541 actatacaat ctctataata agagataagg gaatagatat gaatgcaatt gaatatatag
      601 taaagaatca tccgagctca aaaattcaat ttttagatgg atggactggt aagggaacta
      661 tatcaaaaga acttgaaaaa gcatgtgatg aacttaatgt taaatttaaa aaatattttg
      721 actcaacact tgctgtatta gctgaccctg ctggatattc aggactttat ggaattagag
      781 aagatttctt aataccaagt gcatgtttaa attcaacagt ttctggttta gtaagtagaa
      841 ctgtactaag agaggattta attggtaaaa atgacttcca tggagctaag ttttatagac
      901 atttaaaaga taaagatgaa tctatgaact atatcgaaac tatagaagaa tgttttaaaa
      961 atcaatttaa gaatatatct gatgaagttg aaaactggga aagtgacatt ataacaagag
     1021 atggatactt tgatgtttta aatataaaag aaaagtataa cataacagat attaacttta
     1081 tcaaaccagg tgtaggggaa acgacaaggg ttttacttag aagagttccg tataagattc
     1141 ttgttaaaga cttaaatgat aaatctttag accatatatt catattagct aaggagaaaa
     1201 atgttgaagt tgaacaaatg gatttaaaag cctataaatg ttgtggaata ataaaaaata
     1261 tgaaagatat ttaggaaata tattagacag gagaatattg aatgattgtt ttttcagatt
     1321 tagacaggag tatcatttac tccaataaat ttttaaatgc tgatagtaaa tatactaata
     1381 tagaaattta tagggaaaaa gaaatatctt atatatcttt agatactata aatttaataa
     1441 agcaaataca atattatggt atgtttatac ctacaactac tagaacagtg gagcaattta
     1501 agcgtataga atttaataaa tatgggattt attttccttg gagtataact tcaaatggag
     1561 gagttatact taaagataat gaaatattga aatcatggag tgaaaaaata gataaactaa
     1621 aatgtgatta tgagcccatt gaaagtatga ttcataaatt taaagactat ttgaatgtag
     1681 atggaataac taattttaaa gttgcagaag atacattttt ttatatagtt gttgatttat
     1741 caagatttaa tttggattca atcaaagaat atacaaatat attggaatca aaaaattgga
     1801 agttttatgt gagtggcaga aaaatatatt ttataccaaa agaaatttca aaagaaaatg
     1861 ctataaaata cttgactaaa gaattaggaa tagaatattt ttatgcagtt ggagattcta
     1921 ttatggacta tggtatgctt aatatctcaa ataaatccta tgtattgaag catggtgata
     1981 taaataaaaa tgaaatagaa aattcattta tatcaagttc tttcaatgga atgagtggaa
     2041 ctgaagagat actatctaac attttagacg agaactgcct acttaatata taatgactga
     2101 tatataaata ataaactaga aaaatctgaa ataaattata gtaaagttta tcatactaat
     2161 ttatttcaga tttttatcaa tttatattta agtaagaact caatatgtta aaatgaaggt
     2221 ggagtcattg cccagactga gatacatacc tcatcatcat aattttcata cacatgtggt
     2281 atagtactgt taaaataaaa actatctcct tcctccaata gatattcctt atttccaagt
     2341 gttatcttca ttttaccttt tattatatag ccacattctt ctccttcgtg tgtcacaaat
     2401 tcactgtttt tttgtgtatg accatctagt gtaacaagta taaattctat ttttttccca
     2461 gataaattag gaacaagaag ttcataaaat tttgtagaat cattagttac tatttttttt
     2521 cgattatttt tctttactac tatatcagag tcttctcttc caatctcttc aaaaaaataa
     2581 cctatattta tatcaagagc ttttgatatt ttccacattg ctacaataga tggaacaacc
     2641 atattacgct caatttgact tattaagcct gtacttaaat ctgaattttt tgctaaagta
     2701 gagatactta tatttttaga ttttctaaga gatctaattt tgcttccaac atctatattc
     2761 attagataca aaccccctca gacatgatta taacatataa tttataaaga ttaataaaat
     2821 aaaaaaaaag ttttacaata caagtatttt ttatgctata ttgaattttc tgaaaagatg
     2881 cattataata gtattaatat tcaatatatt gaatattgat aacgatattt taatttaatg
     2941 aaatggaggt aattaagatg aagagttttg taagtcctga taatatttgg gtattatggg
     3001 cagttgtaac tggatgggca gcatttagta tttatttgga acaaaagtac aattgggcat
     3061 caaaagtgtc gggagcaata atagctcttg taggagcaat gttattatca aatctaaata
     3121 ttataccagt agagtctgtt gtatatgacc aagtatgggg atatgttgtt cctttagcca
     3181 tagctttatt gttatatcaa tgtaacatta aaaagatatg gaaagaaagt ggaagattgc
     3241 tcattatatt tcttgtaggt tctgtaggaa ctgtacttgg agctatgatt ggatttttag
     3301 cattaaaaaa tgtagtacca gatttaaata ttgttgcagc tatgatgaca ggttcttata
     3361 taggtggaaa tgtaaacttt gctgctatgt caggggcgtt tgatgcgcca ggagaattag
     3421 tatcagcaac agttgtagca gataatcttt taatggcatt atatttcttt gtcttaatag
     3481 ctataccatc aattggattt tttagaaaac attttaaaca tccacatgta gatgaaatgg
     3541 aaagtatagg tataactgaa ggagaaacag tagcagcaaa ttattgggga agaaaagaaa
     3601 tatctttaaa agatatagca atagccattg gtagcgcctt tataattgtt gcagtatctg
     3661 tagaactatc aacttggttt aaagctataa taccattatc aaatcctttt ttagcaatgt
     3721 taaatacttt atttggaaat caatatctaa taataactac tttgactatg ttgtttgcca
     3781 catttatgcc taatttcttt ggtgatataa aaggagcaca ggaattgggt acatttttaa
     3841 tatatatatt ctttgttgta gtaggtgttc ctgcttcaat aacattaata attcaaaaat
     3901 caccattact acttttatac tgcggaataa tggtactaat aaacatgcta gttacattta
     3961 tagttgcaaa aatatttaag tttagtttag aagaagctat tcttgcatct aatgcaaata
     4021 ttggtggtcc tacaacagca gcagctatgg caatatcaaa aggatggtct aagctagtag
     4081 ggccaatact tatagttgga acatttggat acatagtcgg taattatttt ggtcttttag
     4141 taggaaatat tttgatataa ttttacgtga aaaaaagttg tttattagaa agatttttat
     4201 ttaattggag gttgttatga ttttcgatgg acattcagat acttgggcag atgtaacaat
     4261 aagaaggctt aacggggaaa aagatgtatt tagaagaatc catcttgata attttaaaag
     4321 agcaaatata gaaggagcta tttttgtaat atggatagac ccaccatatg ataaagagcc
     4381 tgaaaaaaga gttaaacaaa ttataagttc tataaaagat gaaattgaag atatgaaaga
     4441 tgttgtaaac atagtaaaaa aatattcaga tatagataaa ggtataagtg aaaacaaaat
     4501 aaatatttta attggtcttg aaggattaag ccatattgga agaaatatag atttactaga
     4561 ttatttttat aaagaagttg gtgcaagaca tgcatcatta acttggaatg aacagaatga
     4621 acttgctaca ggatgtttag gggatgaaaa tcgtggttta agtgaaatag gcaaaaaagc
     4681 agtcaataaa atggaaaaat tgggcatgat agtagatgtg tcacatttaa atgaaaaatc
     4741 attttgggat ttaatgtctg ttgcaagtaa gccagttata gcttctcatt ctaatgctag
     4801 gagtttatgt gatgctaaaa gaaatcttac agataatcaa ttaaaagaaa tttcaaatac
     4861 aggtggatta gttggaataa atagttttag aggatttact aatagtaatc ctaagaagca
     4921 agatttaata catttggttg accatataga ctatatggta aatcttatag gtatagaaca
     4981 tgtatctttt ggttttgatt ttagtgatta cttagggaaa gagactatag gttcttttac
     5041 tggaaatgct agtgtgccaa gtgtagatgg tcttgaaagc acacatacag cacagaacat
     5101 agtagtagag cttgaaaata gaggttataa aaagaaagat atagagttaa tctctagtgg
     5161 taatttttat aggctcttta aagatatact aaaataatat a
//
LOCUS       DAELLV010000237         5167 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.237, whole genome shotgun sequence.
ACCESSION   DAELLV010000237 DAELLV010000000
VERSION     DAELLV010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.237"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            207..2078
                     /locus_tag="KPD58_003184"
     CDS             207..2078
                     /locus_tag="KPD58_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG0266915.1"
                     /translation="MNRKNLYVIMAAAMVSTSVAPSFAAEATQVKKQTITKKEATELV
                     SKVRELMAQRYTGGSQVGQPIYEIKVGETSSQLKIITNIDELEKLVNALGENKELLVT
                     IADKGHTTNSANEVVAEAIEKYENSADLSAEANSITEKAKTEATGIYGVATVEASYDS
                     TKDKLVITLKDKTDVVTSKTLQIGIGDEKVDLTANPVDIAGTNLDPSAEGFRVSKINK
                     LGVEGAKNIADIQLAEITIKNSDLNTVSPQDLYDGYRLTIRGNTIANSVGKSISDISS
                     KDSETGKYKFTVKYTDTSGKAIELTVESTNDKELKDVKDALNGNTKVNLIAGEDRYAT
                     AVAIAKETEYTDNIVIVNSNKLVDGLAATPLAQSKKAPILLASDNEIPKVTLDYIKEI
                     IKKSPSAKIYIVGGESAVSNTARKQLESVTKNVERLAGDDRHTTSVAVAKAMGSFKEA
                     FVVGAKGEADAMSIAAKAAELKAPIIVNGWNDLTADAIKLMDGKEIGIVGGSNNVPSQ
                     IENQLADIDKDRKVQRVEGETRHDTNAKVIETYYGKLDKLYIAKDGYGNNGMLVDALA
                     AGPLAAGKGPILLAKNDITNSQKNTLDKKLNLGAEVTQIGNGVELTVVQKIGKILGW"
     gene            2187..2894
                     /locus_tag="KPD58_003185"
     CDS             2187..2894
                     /locus_tag="KPD58_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBG0266916.1"
                     /translation="MIEATLFFSPHQDDETLSMGSAIIEHVERSDTHVILCTDGSKSI
                     IRKVLDDGGSCSYHIKDVHKYSLSESDFSKNRDEEFKNSCKAMGVKENNIHIENNRAH
                     DGELSKEKARDIMLKYLEKYPDAKVKTVTPYKALGIHEDHRALGEAALELYREGKIKD
                     LRFYVEPYDYNDFKKVNPNVRVWKVLSSQEEKLLNAMDAYKKWNPENGNYAIGYHSVK
                     SHFDELKSNKIQYVHAP"
     gene            2927..5059
                     /locus_tag="KPD58_003186"
     CDS             2927..5059
                     /locus_tag="KPD58_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG0266917.1"
                     /translation="MKISKRIMSLLTATFFIFALSINIADASSKESLVGSGRWETAIK
                     ISQNGWKDATNAILVNDNSIADALSATPFAKLKDAPILLTQSDKLDSRTKAELKRLGV
                     KNVYLIGGMNTLSTNIEKQLKSENMTFERISGNSRYETSLKLAERLDKEKSISDIVVV
                     NGEKGLADAVSVGSAAAQENMPIILSDPNNGIKVANSFISSKNIKKSYIIGGTYSVSS
                     SIEQSLPNVKRLSGNNRNETNAKVLEEFYKNTDLKNAYITKDGMKKQGDLIDSLAVGV
                     LAAKNSSPVVLVGNSLDSSQYDVLNTKSFDNIVQVGGNGNEDAVKDILDLQEETIYRV
                     KTADELDVALRKADANDIITFKPDDNKKITSSFSIETKKAITVELEGLYTQTITVNMP
                     NGELTNKGTLDGTVKIMDIKNGTFINEGDINDIDIYDPNGCKIENKSGGDIWVLTISS
                     SAKDVNIENKGDISKVTNNSTSVIIRNSGNIDTLSGINAPAISGNKPRVDDTDKDEKR
                     ASGIYPTSEPLSPARAGYISVKIFTKPEDTNYKIYYRVVSSRPSALYIGDRIDTSDWT
                     AVPSSVLPFTIKAENGYYIEAVEINSDTKKVSRWGKSDYIDDGFYLKSETAKGLSADI
                     DFDGDYVKLTATGADSGCTVYYRIVSKKPVSMNVGDKVTISDWDEITTNRVRIEANDV
                     KDKYIELVELDDSTNKVTRWGASDKVVE"
BASE COUNT         2137 a          562 c          973 g         1495 t
ORIGIN      
        1 tgagttatct ttatgattaa aaaaaaaaat tacaattttg ttacttttta gtatacaata
       61 ataaatttaa attgactttg ttaattttta ttaataaaat atccaaatat tacaatatct
      121 acagtttatt gaaataatgt gtaaattata ttataattgg taaataagga aaaatggtga
      181 aaaccaaatt ttgtaggggg aaatatatga atagaaaaaa tctttatgta attatggcag
      241 ctgcaatggt aagtacatca gtagctccaa gttttgctgc agaagctaca caagtaaaga
      301 aacaaacaat aactaaaaag gaagcaacag aattagtttc taaagttaga gaactaatgg
      361 ctcagagata tacaggaggt tctcaagttg gacaaccaat atatgaaata aaagttggtg
      421 aaacttcatc acaattaaaa ataataacta atatagacga attagagaaa ttagtaaatg
      481 ctttaggaga aaataaagag cttcttgtga ctatagcaga taaaggacat acaacaaata
      541 gtgcaaatga agtggttgct gaagcaattg aaaaatatga aaattcagca gacctttcag
      601 ctgaagctaa ttcaataaca gaaaaagcta aaactgaagc tactgggatt tatggagtgg
      661 caactgtaga agcttcatat gacagtacaa aagataagtt agtaataact ttaaaagata
      721 agactgatgt agtaacttct aaaacgctac aaataggtat tggtgatgaa aaggttgatt
      781 taacagcaaa tccagttgat atagcaggaa ccaatttaga cccttctgca gaaggattta
      841 gagtaagtaa aataaataaa ttaggtgtag aaggagctaa aaatattgct gatattcaat
      901 tagctgaaat aactataaaa aatagtgatt taaatacagt ttcacctcaa gatttatatg
      961 atggatatag attgactatt cgaggtaata caatagcaaa tagtgtagga aaatcaatta
     1021 gtgatatttc atcaaaagat tcagagacag gaaagtataa atttactgtt aaatatactg
     1081 atacatctgg aaaagcgata gaacttactg tagaaagtac taatgataaa gagttaaaag
     1141 atgttaaaga tgccttaaat ggaaatacaa aagtcaattt aatagctggg gaagacaggt
     1201 atgcaactgc tgtagctata gcaaaagaaa cagaatatac tgacaatata gttatagtta
     1261 attcaaataa attagttgat ggattagcag caacaccact agctcaatct aaaaaagctc
     1321 ctatattgtt ggcgtctgat aatgaaatac caaaagtaac tttagattat ataaaggaaa
     1381 taattaaaaa aagtccatca gctaaaatat atatagtagg tggagaatca gcagtatcaa
     1441 atacagctag aaagcaatta gaatcagtaa ctaagaatgt tgaaagattg gctggagatg
     1501 atagacatac aacttcagta gcagtagcaa aagctatggg ttcttttaaa gaagcatttg
     1561 tagtaggtgc aaaaggggaa gctgatgcta tgagtatagc tgctaaagct gctgaactta
     1621 aggctcctat aatagtaaat ggttggaatg atcttacagc agatgctatc aagttgatgg
     1681 atggaaaaga gattggtata gttggtggtt ctaataatgt acctagccaa attgaaaatc
     1741 aacttgctga tattgataaa gatagaaagg ttcaaagagt tgaaggtgaa acaagacacg
     1801 atactaatgc taaagttata gaaacatatt atggcaaatt agataaacta tatatagcaa
     1861 aagatggata tggaaataat ggtatgttag tagatgcatt ggcagcaggt cctctagcag
     1921 caggaaaagg accaattctt ttagctaaaa atgatataac aaactcacaa aagaatactt
     1981 tagataaaaa actaaatctt ggtgcagaag taactcaaat aggtaatggg gttgaattga
     2041 ctgtagttca aaaaataggc aaaatactag gatggtaata atcaataata tagaaaagga
     2101 ttccttatta tttataggaa tctttttcta tcgacataat attcattaaa gtataacatg
     2161 ctcttgacag ataggaggat atgatagtga tagaagcaac attgtttttt tccccacacc
     2221 aagatgatga aacattaagt atgggtagtg caataattga acatgtagaa agaagcgata
     2281 cacatgttat attatgtact gatggcagta aatcaataat aagaaaagta ttagatgatg
     2341 gagggtcatg ttcttatcat attaaagatg tacacaagta ctctttatca gaaagtgatt
     2401 tttcaaaaaa tagagatgaa gaatttaaaa atagttgtaa agcaatggga gttaaagaaa
     2461 ataatataca tatagaaaat aatagagctc atgatggaga attgagtaag gaaaaagcaa
     2521 gagatataat gctaaaatat ttagaaaaat atcctgatgc taaagtgaag accgttacac
     2581 catataaagc tcttggaata catgaagacc atagagcatt aggtgaagct gcattagaat
     2641 tgtatagaga aggaaaaata aaagatttaa gattttatgt agagccatac gattataatg
     2701 attttaaaaa agttaatcca aatgttagag tatggaaggt tttgagtagt caagaagaga
     2761 aattattaaa tgctatggat gcatacaaga aatggaatcc tgaaaatggt aactacgcaa
     2821 ttggatatca ttctgtaaaa tctcactttg atgaattaaa atccaataaa atccaatatg
     2881 tacatgctcc ataaacatta atataaaaac taggaggata tagttaatga aaatatctaa
     2941 gagaataatg tcgttgttga cagcaacttt ttttatcttt gcattatcta tcaatatagc
     3001 tgatgcttca tcaaaagaga gtctagtggg ctctggtaga tgggaaactg caataaaaat
     3061 tagtcagaat ggatggaaag atgctactaa tgctatacta gtaaatgata attctatagc
     3121 tgatgcatta tctgcaactc catttgctaa gttaaaagat gcacctattt tattaactca
     3181 aagtgataaa ttggatagta gaactaaagc agaattaaaa agactaggtg taaaaaatgt
     3241 atatttaatt ggaggtatga atactctaag caccaatatt gaaaaacaat taaaatcaga
     3301 aaatatgact tttgagagga tttcaggtaa tagtagatat gaaacatctt taaaattagc
     3361 tgaaagatta gataaagaga agagtatttc tgatatagtt gtagttaatg gagagaaagg
     3421 gctagcagat gcagtaagtg tgggttctgc tgcagcacaa gaaaatatgc caattatact
     3481 ttcagatcct aacaatggaa taaaagttgc aaatagcttt attagtagta aaaatataaa
     3541 gaaatcttat ataattggtg gtacatattc tgtatctagt tctattgaac agagtttacc
     3601 taatgtaaaa agattatctg gaaacaatag aaatgaaact aatgcaaagg ttctagaaga
     3661 attttataaa aatacagact taaaaaatgc ttatataact aaagatggta tgaaaaaaca
     3721 gggggatttg atagattctc tagctgtagg tgtattggca gctaaaaata gctctccagt
     3781 tgtattagta ggaaattcac ttgactctag ccaatatgat gtgttaaata ctaaaagttt
     3841 tgataatatt gtgcaagttg gtggaaatgg aaatgaagat gctgtaaagg atattcttga
     3901 tttacaagaa gaaactatat atagagttaa gacagcagac gaattggatg ttgctctaag
     3961 aaaagcagat gctaatgata ttataacatt taaacctgat gataataaaa aaattactag
     4021 ttcatttagt atagaaacaa agaaagctat aactgtagag cttgaaggat tgtatacaca
     4081 aacaataact gtaaatatgc caaatggaga attaactaat aagggtacat tagatggaac
     4141 tgtgaaaata atggatataa aaaatggcac atttatcaat gaaggtgata taaatgatat
     4201 tgacatttat gatccaaatg gatgtaaaat agagaataaa agtggtggag atatatgggt
     4261 tttaactata tctagtagtg ctaaagatgt aaatatagaa aataaaggtg atataagtaa
     4321 ggttacaaat aattctacta gcgttataat aagaaattca ggaaatattg atactttaag
     4381 tggaattaat gcaccagcaa taagtggaaa taaacctaga gttgatgata ccgataagga
     4441 tgaaaaaaga gcaagtggaa tatatcctac ttcagaacct ttatcacctg caagagctgg
     4501 ttatataagt gtaaaaatat tcactaagcc tgaagataca aattataaaa tttattatag
     4561 agtagtaagc agtagaccat ctgcattata tatcggagat agaatagata caagtgactg
     4621 gactgctgta ccatcttctg ttttaccatt tactattaaa gcagaaaatg gatattatat
     4681 agaagctgtt gaaataaatt ctgatactaa gaaagtatct agatggggga aatctgacta
     4741 tatagatgat ggattttatt taaaatcaga aactgcaaaa ggattaagtg cagatataga
     4801 ttttgatgga gattatgtta aattaacagc aacaggtgct gattctggat gtacagttta
     4861 ttatagaata gtatcaaaaa agccagtatc aatgaatgtt ggagataaag taactatatc
     4921 tgattgggat gaaattacta ctaatagagt gaggatagaa gcaaacgatg taaaggataa
     4981 atatatagaa ctagttgaat tggatgattc aactaataaa gtaactagat ggggagcaag
     5041 tgataaggta gtagaatagg tatctaatgt gaaacataaa aacagactga ttaataatgg
     5101 gcagtctgtt tttatttcct aaaaattatc attttactaa ataaataatt taaaactata
     5161 acaatta
//
LOCUS       DAELLV010000238         5150 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.238, whole genome shotgun sequence.
ACCESSION   DAELLV010000238 DAELLV010000000
VERSION     DAELLV010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5150
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.238"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            44..1063
                     /gene="hrcA"
                     /locus_tag="KPD58_003187"
     CDS             44..1063
                     /gene="hrcA"
                     /locus_tag="KPD58_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HBG0266918.1"
                     /translation="MELNERKLNILKAIVKDYIETAEAIGSRTISKRHDLGVSAATIR
                     NEMADLEELGYLIQPHTSAGRVPSEKGYKLYVNSLMSKSELDDNDKILIEQCMNHNIN
                     HIKELIHETSKLLSQLTNYTTVAVTKSLINQSVIKHIQLVAMNDNNILLIVVTDKGDL
                     KKANLTTNVYLDQSKLNLISDNLTRKLLGKSITDLDDNLIAFIKYEISEYSGLIDELL
                     NALNSNMKEEDFSLSLNGATNIFSYPEFNDVLKAKSFLNMLEKKETIADIIKSKGIQK
                     DNLNIIIGSDNDCELAQDCSIVTATYNVDRDLVGRISFIGPTRMDYARIYSIINYMSL
                     LINRK"
     gene            1093..1713
                     /gene="grpE"
                     /locus_tag="KPD58_003188"
     CDS             1093..1713
                     /gene="grpE"
                     /locus_tag="KPD58_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBG0266919.1"
                     /translation="MDKKNEQQEVREENDTSINQESETQVELEEEVVNEECETSSEKT
                     DEKEVDDENVTDINSKLAEKKLQDELDELNDKYQRLQAEYANYRRRTQQEKETIGVFA
                     NEKIITELIPVIDSMERALDACEDKEDTMYKGISLVHKQLIDTLVKFGVEEIEAESKE
                     FDPNLHLAVMQESVDGVEANQIVMVLQKGYKLGTKVVRPSMVKVSC"
     gene            1805..3652
                     /gene="dnaK"
                     /locus_tag="KPD58_003189"
     CDS             1805..3652
                     /gene="dnaK"
                     /locus_tag="KPD58_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBG0266920.1"
                     /translation="MGKIIGIDLGTTNSCVAVLEGGEAQIIANSEGMRTTPSVVAFTK
                     DGERIVGEPAKRQAVTNADKTITSIKTHMGTDYKVNIDGKSYTPQEISAIILQKLKSD
                     AESYLGQTVTEAVITVPAYFTDAQRQATKDAGRIAGLDVKRIINEPTAAALAYGMDKL
                     DQEKKILVFDLGGGTFDVSILEIGDGTFEVLATAGNNRLGGDDFDQIVIDYLAEEFKK
                     AEGVDLRNDKMALQRLKEAAEKAKKELSSTMSSNINLPFITATAEGPKHLNIDLSRAK
                     FEELTRGLVEKTMEPTKTALQDAGLSTGDIDDVLLVGGSTRIPAVQEAVKKFIGKEPH
                     KGINPDECVAAGASIQAGVLAGDVKDLLLLDVTPLSLGIETMGNVMTKIIERNTTIPT
                     KKSQIFSTAADNQTAVDIHVLQGERSMAYDNTTLGRFQLTDIPPAQRGIPQIEVTFDI
                     DANGIVNVSAKDLGTGKEQKITITSNTNLSEAEIEQKIKEAEMNAEADKQKKEKIEAF
                     NQAESTIYQTEKTLNELGDKISSGEKEDIEKAIADLKAVKDNQDATAEELKKATDEVM
                     TKFQKVSQEMYQKAAQEQQAAQGAEQAQDNGPKDDNVVDADFKEVDEDK"
     gene            3863..5017
                     /gene="dnaJ"
                     /locus_tag="KPD58_003190"
     CDS             3863..5017
                     /gene="dnaJ"
                     /locus_tag="KPD58_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBG0266921.1"
                     /translation="MSTKRDYYEVLGISKGAEAQEIKKAYRKLAMKYHPDRNPGDKEA
                     EEKFKEINEAYEVLSDDTKRKTYDQFGHDGLNGQGGFGGQGGFGGQGFGGFEDMFGDI
                     FGDMFGGSFGGGRARRRGPQRGADIRQSVTISFEEAAFGKKMSIKVNRSEECEECNGT
                     GAKPGTSKKTCSTCNGTGQVRTVQRTPFGNIASSRPCSACNGTGEVIESPCSKCHGTG
                     NTRKVKTIEVDIPAGIDDGQMIKLSGQGEVGEKGAPRGDLYIVVNVKSHPLFTRDGND
                     IYFEMPITFVQATLGDEIEVPTLDGKVKYSVPEGTQTGTVFRLKEKGIPRIRGNSRGD
                     QYVKVVVEIPKKLNDKQKELLREFAKECGSNVHEKKKTFGQKIEDMFKKK"
BASE COUNT         2141 a          596 c         1017 g         1396 t
ORIGIN      
        1 cactcgaact tagtgagtgc taacagctag gaggtataat aatatggaat taaatgaaag
       61 aaaattaaat atcctcaaag ctatagttaa agattatata gaaacagctg aagctatagg
      121 ttctagaaca atatctaaaa gacatgattt aggtgttagt gctgctacca taagaaatga
      181 aatggctgac cttgaagaat tagggtattt aattcagcct catacttctg cgggtagagt
      241 tccatctgaa aaaggctata agttatatgt aaactctctt atgagtaaaa gcgaattgga
      301 tgataatgat aagattctta ttgaacaatg tatgaatcac aatataaatc atataaaaga
      361 attgattcat gaaacttcta agctattatc gcaactaaca aactatacaa ctgtagcagt
      421 tactaagagt ttaataaatc aaagtgttat aaagcacata cagttggttg ctatgaatga
      481 caataatatt ttacttatag ttgtaacaga taaaggggac ttaaaaaaag ctaaccttac
      541 aactaatgta tatttagatc aatctaagtt aaatttgatt tctgataatc ttacaaggaa
      601 gctcttaggt aaaagtataa cagatttgga tgataatcta attgctttta ttaaatacga
      661 aataagtgag tattctgggc ttatagatga gcttttaaat gctttgaact caaatatgaa
      721 agaagaagat ttttctctat cattaaatgg agctactaac atatttagtt atccagaatt
      781 taatgatgta ttaaaagcaa agtcattttt aaatatgttg gaaaaaaagg aaacaatagc
      841 tgacataatt aaatcaaagg gaatacaaaa agacaatcta aatataataa ttggtagtga
      901 taatgattgt gaactggcac aggattgtag tatagttaca gcaacttata atgtagatag
      961 agacttggtt gggcgaataa gctttatagg ccctaccaga atggattatg ctagaattta
     1021 ctctataata aattatatga gtttattaat taatagaaag taaagaaatt ttaaattgcg
     1081 aggtgtaaag tcttggataa aaaaaatgag caacaggaag taagagaaga aaatgatact
     1141 tctattaacc aagaatcgga aactcaagta gagttagaag aggaagttgt taatgaagaa
     1201 tgtgaaacat caagtgaaaa aactgatgaa aaagaagttg atgatgaaaa tgtaacagat
     1261 ataaattcta agttagcaga aaaaaaattg caggatgagt tggatgaatt aaatgataaa
     1321 tatcaaagac ttcaagccga atatgcaaac tacagaagaa gaactcaaca agagaaagag
     1381 acaataggcg tatttgcaaa tgaaaaaata ataactgaat tgataccagt tatagatagt
     1441 atggaaagag ccttagatgc atgtgaagat aaggaagata ctatgtataa gggtataagc
     1501 cttgtacata aacaattaat agacacttta gtaaaatttg gagtagaaga aatagaagca
     1561 gaatctaagg aatttgaccc taatctacat ctagcagtta tgcaagaaag tgtagatgga
     1621 gttgaagcaa atcaaatagt aatggtactt caaaaaggat ataagctagg aactaaagtt
     1681 gtaagacctt caatggttaa agtttcttgc taagtataaa ttaaaggtta aattatttaa
     1741 cctaatataa aatattaaaa taagtaaaac ttaattatat aattactcga ggaggatttt
     1801 agtcatggga aaaataatag gaatagattt aggaacaaca aattcatgtg ttgcagtgtt
     1861 agaaggtgga gaagcacaaa taatagcaaa tagtgaaggt atgagaacta ctccatcagt
     1921 agtagcattt acaaaagatg gtgaaagaat agttggagaa cctgcaaaaa gacaagcagt
     1981 tacaaatgca gataaaacaa taacttctat aaaaactcat atgggaactg attataaagt
     2041 aaatatagat ggaaaatcat atactccaca agaaatatca gcaataatat tacaaaaatt
     2101 aaaatcagat gcagaaagtt acttaggaca aacagtaaca gaagcagtta taacagtacc
     2161 agcttacttt acagatgctc aaagacaagc aactaaagat gctggtagaa tagcagggct
     2221 tgatgttaag agaataataa atgagccaac agcagcagct cttgcttatg gtatggataa
     2281 attagaccaa gaaaagaaaa tattagtatt tgacttaggt ggaggaactt ttgacgtatc
     2341 tatacttgaa ataggagatg gaacttttga agttttagct acagctggta acaatagatt
     2401 aggtggagat gactttgacc aaatagtaat agattattta gcagaggaat ttaaaaaagc
     2461 tgaaggtgta gatttaagaa atgataaaat ggctcttcaa agattaaaag aagcagcaga
     2521 aaaggctaag aaagaattat catcaacaat gagttcaaac ataaacttac ctttcataac
     2581 tgctacagca gaaggaccaa aacacttaaa tatagattta tcaagagcta aatttgaaga
     2641 attaactaga ggtttagttg aaaaaactat ggaaccaact aagacagctt tacaagatgc
     2701 aggactttct acaggtgata tagatgatgt attattagta ggtggttcta caagaatacc
     2761 agcagtacaa gaagctgtta agaagtttat aggaaaagaa cctcataaag gtataaaccc
     2821 agatgagtgt gttgcagcag gtgcttctat acaagctgga gttttagcag gggatgttaa
     2881 agacttatta ttactagatg ttactccatt atctcttggt atagaaacta tgggtaatgt
     2941 aatgactaag ataatagaaa gaaatactac aataccaact aagaagtctc aaatattctc
     3001 aactgctgct gataaccaaa cagctgttga tatacatgtt cttcaaggtg aaagaagtat
     3061 ggcttatgac aatacaactt taggaagatt ccaattaaca gatataccac cagcacaaag
     3121 aggaatacct caaatagaag ttacttttga tatagatgca aatggtatag ttaatgtttc
     3181 agctaaagat ttaggtacag gaaaagagca aaaaataact ataacatcaa acactaattt
     3241 atcagaagca gaaatagagc aaaagataaa agaagcagaa atgaatgcag aagctgacaa
     3301 acaaaagaaa gaaaaaatag aagcattcaa tcaagctgaa tctactatat atcaaactga
     3361 aaaaacttta aatgaacttg gagataaaat aagttcaggt gaaaaagagg atatagaaaa
     3421 agctatagca gatttaaaag ctgtaaaaga taatcaagat gctacagcag aagaacttaa
     3481 gaaagctaca gatgaagtaa tgactaagtt ccaaaaagta tctcaagaaa tgtaccaaaa
     3541 agcagctcaa gaacaacaag cggcacaagg tgcagaacaa gcacaagaca atggacctaa
     3601 agatgacaat gtagtagatg ctgactttaa agaagtagat gaagataaat aagctatata
     3661 atatgtaaca ctaaatgata attggaacat atatgatttg tgaaaatcat atagatgtta
     3721 aagatatatg gacaatatac atcataaaga atataatata tcatagatta tataacttaa
     3781 agtagaatat aagaattaat gatagcttgt agtttattat tctacaagct atcgttttgt
     3841 aaaatgaagg tggtgtcaat ttttgagtac aaaaagggat tattatgaag tcttaggtat
     3901 cagtaaaggt gcagaagctc aagagataaa aaaagcttat agaaaattag caatgaagta
     3961 ccatccagat agaaatcctg gagataaaga ggcggaagaa aaatttaaag agataaatga
     4021 agcttatgaa gttctatctg atgatacaaa gagaaaaact tatgaccagt ttggacatga
     4081 tgggctaaat ggtcaaggcg gttttggtgg tcaaggagga tttggcggtc aaggctttgg
     4141 tggttttgaa gatatgttcg gagatatatt tggtgacatg tttggaggaa gcttcggagg
     4201 tggaagagca agaagaagag gacctcaacg tggtgcagat ataagacaga gtgttactat
     4261 atcttttgaa gaagctgcat ttggtaaaaa gatgtctata aaagtaaata gaagtgaaga
     4321 gtgtgaagaa tgtaatggaa ctggagcaaa accaggaaca tcaaagaaaa cttgttcaac
     4381 ttgtaatgga actggtcaag ttagaactgt tcaaagaact ccatttggta atatagcaag
     4441 ttcaagacca tgtagtgctt gtaatggaac tggagaagtg attgaatctc catgtagtaa
     4501 atgtcatgga actggaaata caagaaaagt taagactata gaagtagata tacctgctgg
     4561 tatagatgat ggtcaaatga taaaactttc tggtcaaggt gaagtaggag aaaaaggtgc
     4621 tccaagagga gacctatata ttgtagttaa tgttaagtca catccactat ttactagaga
     4681 tggaaatgat atttattttg aaatgccaat tacatttgtt caagcaactt taggtgatga
     4741 gatagaagta cctactttgg atggaaaagt taaatatagt gtgccagagg ggacacaaac
     4801 tggaactgta tttagactta aagagaaagg aatccctaga attagaggta attcaagagg
     4861 agaccaatat gttaaggttg tagttgaaat tcctaaaaaa ttaaacgata agcaaaaaga
     4921 actattgaga gaatttgcga aggagtgtgg gtcgaacgtc cacgaaaaga agaaaacctt
     4981 cggacaaaag atagaagata tgtttaagaa aaagtagtta aagtagccaa tatataatca
     5041 tatttttaaa atcttagtta tttataagat tgtcaaagga gatattatga tttttatatt
     5101 ggctattttt gtataaaaaa taaatgtgca taaaataaaa ctgaatataa
//
LOCUS       DAELLV010000239         5143 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.239, whole genome shotgun sequence.
ACCESSION   DAELLV010000239 DAELLV010000000
VERSION     DAELLV010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5143
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.239"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            158..1081
                     /locus_tag="KPD58_003191"
     CDS             158..1081
                     /locus_tag="KPD58_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HBG0266922.1"
                     /translation="MGSGKVGTSLANYFLSKGYCVSGFYGRNQLSLLESTNLTKTKIY
                     SNLKDIIYENDILFITTSDDSIEIIDKKLSKFNLKHKYICHTSGSLKSSILFCSKKAG
                     ALIYSIHPIFAFSNKNTNIKKMKDICFSIEGDNEQDDLVIQNFIDDLGNQFFIRDKDT
                     SSTYHLANVLVSNLVLSLLDIGVSYFIKLGLSEEESLKAISPLVKKNIENILDNGFTK
                     ALTGPVSRGDITPIRKHLSVLDKKDEDIYKILSLNLLKIIALGNDNSLKGIKNITIEN
                     AIENLISKSEKYKEIYKLLGGKNDEKYHTDF"
     gene            1056..1883
                     /gene="panB"
                     /locus_tag="KPD58_003192"
     CDS             1056..1883
                     /gene="panB"
                     /locus_tag="KPD58_003192"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HBG0266923.1"
                     /translation="MKNTIQTFKNAKYEGKKLSMLTAYDYSIAKIMDECDINGILIGD
                     SLGMVIKGEENTLSVTIDEIIYHTKAVKNGVKNALIVSDMPFLSYHVSIEDAVKNAGR
                     LIKEGGAHAVKLEGGSNVIKQIESIVNAQIPVMGHLGLTPQSVNSFGGFKVQGNTSET
                     ARQLIEDAKLIEKAGAFSIVLEGVPTKIAEMVTNSISIPTIGIGAGINCDGQILVYQD
                     MLGMFGDFVPKFVKQYANIGDIMKDSIKNYILEVNTGAFPQEKHSFSINESELEKLY"
     gene            1902..2750
                     /locus_tag="KPD58_003193"
     CDS             1902..2750
                     /locus_tag="KPD58_003193"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HBG0266924.1"
                     /translation="MLVKEIKLLRNIIKDWRKHGYSIGLVTTMGFLHEGHQSLIKKAI
                     KENDKVVVSVFVNPTQFGPNEDFNSYPRDIDKDFKYCMDSGATVVFNPSPEEMYLKGN
                     CTTINVSGLTDFLCGAKRPVHFGGVCLVVSKFLNIVTPDKAYFGEKDAQQLAVIKRMV
                     KDLNIDTEIIGCPIIRENDGLAKSSRNTYLSEEERKSALILNKSLSLAKEELVKGNLN
                     PENIKELIIAKINSEHLAKIDYVEIVDSETLQPVKQIEHSILVAIAVFIGKTRLIDNF
                     TFELNI"
     gene            3161..3343
                     /locus_tag="KPD58_003194"
     CDS             3161..3343
                     /locus_tag="KPD58_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266925.1"
                     /translation="MFKDKIDECVHIMTAYIASLKEYYSFIETQIGDFIKKYGEDVVE
                     LCLHRVMILLCECGLA"
     gene            4175..5089
                     /locus_tag="KPD58_003195"
     CDS             4175..5089
                     /locus_tag="KPD58_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HBG0266926.1"
                     /translation="MIDNQKYVILSLELHLFFSRIMKEHALFLEAGFTNKNYNLAMEA
                     DHYKKQFEDLLSYTVSASNGIIRPDILYSEELVTTLTSVAEQKTEEFTGIEINKNITT
                     RELNLQSGVNPQVGQDLVNYVAQLNSDAIRLLDGLINFKERVLDGVLSCTIFTSNYPL
                     LLEHIIHEANLYRSYVVDLENKIDIESKNAKEIELFWDHIMMEHALFMRGLLDPSEGE
                     LINTSNDFAIKFNELIEKTNEMTDSNIKNITEETLNETVEFKDFKEAGASGIEQCKIK
                     SIILPLLADHVLREANHYIRILESYKNM"
BASE COUNT         2041 a          622 c          739 g         1741 t
ORIGIN      
        1 aaaatggtaa gatatttatg gatggtatct tgttataaga actatacttt aaatgttttt
       61 aattattttg tcttatgtaa ctcatatcct caggaatgag attagaaatt aagatgttgt
      121 atcacaaagt ggaggtgata ctattaaaat aggctttatt ggttctggaa aagttggaac
      181 atctttggca aattattttt tatcaaaagg ttattgtgtt tcaggctttt atggaaggaa
      241 tcaattatct ttattagaat caacaaattt aacaaaaaca aagatttaca gcaatttgaa
      301 agatataatt tatgaaaacg atatattatt tattacaaca tcagatgatt ctattgaaat
      361 tatagacaaa aaactttcca aatttaattt aaaacataaa tacatttgcc acacaagtgg
      421 ctctttaaaa tctagtatat tgttctgttc aaaaaaagca ggtgcattaa tttattctat
      481 ccatcctata tttgcatttt ccaataaaaa taccaacata aaaaaaatga aagacatttg
      541 tttttccata gaaggtgata atgaacaaga tgatttagtt attcaaaatt ttatagatga
      601 tttgggaaac caatttttta taagagataa ggatacttca tccacttatc atttagctaa
      661 tgttttagtt tcaaatttag tattatcttt actagatata ggagtaagtt actttattaa
      721 attaggttta tctgaagagg aatctttaaa agctatcagt cctttagtaa aaaaaaatat
      781 agaaaatata cttgataatg gctttactaa agctcttact ggtcctgttt ctcgtggaga
      841 tataacacca ataagaaaac atctatctgt attggataaa aaagatgagg atatttacaa
      901 aatactatcc ttaaacctac taaaaataat tgccttagga aatgacaatt cattaaaagg
      961 tataaaaaat attactatag aaaatgcaat cgaaaattta ataagtaaat ctgaaaaata
     1021 caaagaaatt tacaaacttt taggaggaaa aaacgatgaa aaataccata cagactttta
     1081 agaatgctaa atatgaagga aaaaaactat ctatgcttac tgcttatgac tactcaattg
     1141 caaaaataat ggatgagtgt gatataaatg gtattttaat aggagattca ttaggaatgg
     1201 tcataaaagg tgaagaaaac actctatctg ttaccataga cgaaattata tatcatacaa
     1261 aagctgtaaa aaatggtgta aaaaatgcct taatcgtaag tgatatgcct tttctatctt
     1321 accatgtttc tatagaagat gcagttaaaa acgctggtag acttataaaa gaaggaggag
     1381 ctcatgctgt aaagttagaa ggtggctcaa acgttataaa gcaaatagaa agcattgtaa
     1441 atgctcaaat acctgtaatg gggcatttag gattaactcc tcaatcagta aattcatttg
     1501 gtgggtttaa ggttcaagga aataccagtg aaacagcaag acaacttatt gaagatgcta
     1561 aattaataga aaaagcaggt gcattctcta ttgtccttga aggagtgcct acgaaaatag
     1621 ctgagatggt aactaattct atttcaattc ctacaatagg tataggggca ggaattaact
     1681 gtgatggaca aattttagtt tatcaagata tgttgggaat gtttggagat tttgttccta
     1741 agtttgtaaa gcagtatgca aatataggag atataatgaa agattcaatt aaaaattaca
     1801 tcctagaagt taatacaggt gcatttccac aagaaaaaca cagcttttca ataaatgaat
     1861 ctgagttaga aaaattatac taatattaag gatggtacaa aatgcttgta aaagaaatta
     1921 aattactaag aaatataatc aaagattgga gaaaacatgg ctattctata ggccttgtta
     1981 caacaatggg atttttacat gaaggacacc aaagtcttat aaaaaaagct ataaaagaaa
     2041 atgataaagt agtagtgagt gtatttgtaa atccaactca atttggacca aatgaagatt
     2101 ttaatagcta tccaagagat atagataaag attttaaata ttgtatggat agtggagcaa
     2161 cagtagtctt taacccatca ccagaagaaa tgtatttaaa aggtaattgt actaccatta
     2221 atgtttctgg acttacagat tttctttgtg gagctaaaag acctgttcat tttggaggtg
     2281 tctgcctagt tgtttcaaag tttctaaata tagtaacacc tgataaagct tatttcggag
     2341 agaaggatgc ccaacaatta gctgtaatta aaagaatggt caaggattta aacatagata
     2401 cagaaataat aggctgtcct ataattcgtg aaaatgatgg attagcaaaa agttcgagaa
     2461 atacatatct ctctgaggaa gaacgaaaat ctgctctaat attaaataag agcttaagtt
     2521 tagcaaaaga agaattagta aaaggcaatc taaatccaga gaatataaaa gaattaataa
     2581 tagctaaaat aaattctgaa cacttagcaa aaatagacta tgttgaaatt gtagatagtg
     2641 agacattaca acctgttaag caaatagaac attctatact tgtggcaatt gctgtgttta
     2701 taggaaaaac cagactaatt gataatttta catttgagtt aaatatatag tctataattt
     2761 tatataatat atctaaaaaa taatagaata cctaaaaagt aataaattac ctaaaaaata
     2821 ataaattgct tagaaaataa tagattacaa taactaaaat tttaaaatta aattccttaa
     2881 aagtatgatt aattataatt atataagtat attcttatta cacttatatt aatttatatt
     2941 aaatatctac taactcaaat attcataatt aaactttaaa ctggataaat tttcttttaa
     3001 gaatgaaagt ttatccagtt ttgcgattat aataaccagt tttacatatc cacttgtaaa
     3061 atatatctaa gcaatatatc atactatata gatttatgag tgaaataata tctgctattt
     3121 actgaatatt tttatttttc gttttttagg gggattagtt gtgtttaaag ataagattga
     3181 tgaatgtgtt catataatga ctgcatatat tgctagttta aaagaatact attcattcat
     3241 agaaactcaa attggtgatt ttattaaaaa atatggagaa gatgttgtag agttatgtct
     3301 ccatagagtg atgattctac tatgcgaatg tggtttggca taaaattaaa tttattaaaa
     3361 tgggtaagaa gaatcctata taatattctt cttacccatt ttaatatatc aatttcatag
     3421 cttatcgttt aaaatatttt acatccatta ctagacaact ttacattctc tttttaattc
     3481 tagtatattt aaatcatttt agttgctcct tatttattct tattacttgt tttattttat
     3541 aaataaaata attcttttta ataatgtttt ttgttgatat tcctatcaca catccaatta
     3601 tttatcattg tcttgaaatt gtttgcatac ttaatttaat ccaattgaaa cactccagca
     3661 tagaatcccc tgtaaaatag aagttggatt aaatcctaac atctatacag aaaatactac
     3721 tccaagcaca agcaatataa tggaatatat ttatcactta attttttata ctttttaaat
     3781 cccataccta aaacattaag tgcagctatt agaattaata attgttgtgg tataaaatta
     3841 tgcttgcttc atatttattc attttttcct ataaaagttc ttgttttaca aaaaacattt
     3901 gttttgtatc aaaacaatac ttataaatta cataattctc ataaaaattt ctaatattac
     3961 ttgtactctt aataaataat agccaacaag caccttaaat cattatatct atatctaata
     4021 agaaacacag taagataatt ttttcgccca taacttaata aaaactgttg atattagtag
     4081 caattgaatt tagttaattt atttataata tacataaatt taaatattac gaataaagta
     4141 ttataaatag gatactttct aaagggagga aattatgata gataatcaaa aatatgttat
     4201 tttatcacta gaattacatt tatttttttc aagaattatg aaagagcatg ctcttttttt
     4261 agaagcagga ttcacaaata aaaattataa tcttgctatg gaagctgacc attataaaaa
     4321 gcaatttgaa gatttattat catacactgt tagtgctagt aatggtataa ttagacctga
     4381 tatattatat tcagaagagc ttgtaactac tctcacatca gttgcagaac aaaaaacaga
     4441 agagtttaca gggatagaaa taaacaaaaa catcactaca agagaattaa atctacaaag
     4501 tggtgtaaac ccacaagttg gtcaagattt agtgaactat gtagctcaac ttaactctga
     4561 tgcaataaga ttacttgatg ggcttattaa ttttaaagaa agagtcttag atggtgtact
     4621 atcatgtact atatttacat caaactaccc tctacttctt gaacatataa tacatgaagc
     4681 aaatttatat cgttcttatg tagttgacct tgaaaataaa atagatattg agtcaaaaaa
     4741 cgctaaagaa atagaattat tctgggacca tattatgatg gaacatgctc tgtttatgag
     4801 aggattacta gacccctcag aaggtgaact aataaatact tcaaatgatt ttgctataaa
     4861 atttaatgaa ttaattgaaa aaacaaacga aatgactgat tctaatatca agaacattac
     4921 agaagaaact ctaaatgaaa ctgttgagtt taaagatttt aaagaagcag gagcatcagg
     4981 aatagaacag tgtaagataa aatctataat attaccactt ttagcagacc atgttttaag
     5041 agaggcaaat cattatatta gaatattgga gagttataaa aacatgtaat taaaaattac
     5101 ttttaaaata atctttgcat atttagataa aataaaggta cag
//
LOCUS       DAELLV010000240         5092 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.240, whole genome shotgun sequence.
ACCESSION   DAELLV010000240 DAELLV010000000
VERSION     DAELLV010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5092
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.240"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            78..881
                     /locus_tag="KPD58_003196"
     CDS             78..881
                     /locus_tag="KPD58_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266927.1"
                     /translation="MEKLRVIEMIKNNPNIIATIDNPTDEMKLLAIKENGLVLEYINN
                     PTREMQELAIDNNIRAIKFIDNPTEDMMIKAVNEGWSILDYIKNPTDKVIEMAINQAG
                     WAIKYAKNPSEELQLLAVRKNYDSIKFIKQPYESVQEEAVRISYDALRYIDSPSYNAE
                     LIAIKNNEAAISFITDLDKNKMLEFLKVNILVIKYILKKVSKDELESVLKEVLSKEDV
                     EEKYIRDFLNCSVIDRNSESIPIDKIMFIYKYGSKTAKKIAVDEKLKIM"
     gene            893..2023
                     /locus_tag="KPD58_003197"
     CDS             893..2023
                     /locus_tag="KPD58_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0266928.1"
                     /translation="MNFIDTLKSVDLILSTGEVPLIVGESGIGKTALANKLAKENDWS
                     LIVIDGNLLKEGEIGGLPTIESYVGVNSNGYKTEKKTTVYAVHNKLREIDEEISKSKT
                     VLLFIDEINRCEHTVQQELMNLILNREINGYKLHDDVKILAAMNPSSKYGSDFDYQVV
                     DMDAAQENRFVWLNMESDHTQWIKWAIDEGIERKVIEFISTFPEYLHKINEDDVRATP
                     RSYERVSKIYKVYKEKNNSIPRAVFLNVVKGNVGKVIAEEFISFIESNSEPLISYEDV
                     FLGESIDESIVERVKNESHTRLYLSAMNILKDLELNIKNDKYESNHYINRFIEFLKMY
                     PVDLMIGIMKDIRNSYIEVYKKAIENEEFVKSYFESYSLIRG"
     gene            2027..3448
                     /locus_tag="KPD58_003198"
     CDS             2027..3448
                     /locus_tag="KPD58_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266929.1"
                     /translation="MENYFDKQAKELYNKASEIINTHSMLKANRANEKFDIDIPQDFK
                     YEFFSLVDKVNLSLMEEEDNFYGYFLFQMSREIRFDISSPTGVNFKGAKYIIYFNPII
                     FLTLDIKQMETTIKHEIHHILSMHLMRAKELKGKYSTLAINMAMDIVVNKFLNNLPPY
                     ATTLEWVNLKYSLKLEPYEPFEYYVEKIQTELDLMEVDEDGEEDGEEDDSDKYGDIEI
                     DYSPERTHDIWDESNEIDENTLREFTEKFINSSQKGKVPAYLESMISSLKNSKGELPW
                     NLYLNRIMGTVENNKKKTITRRNRRQPNRLDLRGELRSHKSEIAVALDVSGSISDEEF
                     KQAIKEVLSIVKNYNHEITIIECDNEIRRVYKVRTEKDIKDRIRIGGGTRFSPVFEYA
                     NNKKINLLVYFTDGKGENKLQVIPRGYKILWVISGRGDKISLKEPYGAVKKLTKVKTN
                     DEILEINDVRNDGYSMNSQAPIL"
     gene            3659..4168
                     /locus_tag="KPD58_003199"
     CDS             3659..4168
                     /locus_tag="KPD58_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HBG0266930.1"
                     /translation="MVMFLFKIDDYIMYGMTGVCKVMDITNERFTNGIKKEYYVLSPI
                     YSNNTVIKIPVDNEKVPMRKLLSKINVLSLINDIPNMDTSWIDNEKLRSEQFKKILRG
                     GKCEELIKLVRSIDNNREYVKSIGKKTHQADDNIMKEAERLLNEEFATILDISPNEVS
                     SYISSHIPQ"
BASE COUNT         2128 a          427 c          870 g         1667 t
ORIGIN      
        1 gaaacaatta tataaatagg aatagattat tataattaat atagatataa aaataattat
       61 agaaggaaag gcacattatg gaaaaattga gagttattga aatgataaaa aataatccca
      121 atattatagc tacaatagat aatccaactg atgaaatgaa attgttagct attaaagaaa
      181 atgggcttgt actagaatat ataaataatc caaccagaga gatgcaagaa ctagcaatag
      241 ataataatat tagagcaatt aaatttatag ataatccaac agaagatatg atgataaaag
      301 cagtaaatga gggatggagt atactggatt atatcaaaaa cccaactgat aaagttattg
      361 aaatggctat aaatcaagca ggatgggcaa ttaaatatgc taaaaatcca agtgaagaat
      421 tacaactgtt agcagtaaga aaaaattatg attcgataaa gtttataaaa caaccttatg
      481 aaagtgtaca agaggaggca gtgagaataa gttatgatgc cttgagatat atagattcac
      541 ctagttataa tgcagaactt atagctataa aaaataatga agcagctatt agctttataa
      601 ctgatttaga taaaaataaa atgttggaat ttttaaaagt gaatatttta gtgattaagt
      661 acattctgaa aaaagtatct aaagatgaat tagagagtgt attaaaagaa gtattatcca
      721 aagaagatgt tgaggaaaag tatattagag attttttgaa ttgtagtgtt atagatagaa
      781 atagcgaaag tataccaata gataaaatta tgtttattta taaatatgga agtaagacag
      841 caaaaaaaat agctgtagat gagaaattaa agattatgta ggagagagaa aaatgaattt
      901 tatagacaca ttaaaaagcg ttgacttaat attatccact ggagaagtac ctttaatagt
      961 aggtgaaagt ggaataggaa aaacagcttt agcaaataag cttgccaaag aaaatgattg
     1021 gagtttaatt gttattgatg gaaacttact taaagagggt gaaataggtg gtcttccaac
     1081 tatagaatct tatgtaggag ttaattctaa tggatataag actgaaaaga aaacaacagt
     1141 gtatgctgtt cataacaaat taagagaaat tgatgaagag atatctaaaa gtaagacagt
     1201 tcttttattt atagatgaga taaatcgttg tgagcataca gtacagcaag aacttatgaa
     1261 tttaatttta aatagagaaa ttaatggata taaactgcat gatgatgtaa agattttagc
     1321 agcaatgaat ccatcaagta aatatggttc agattttgat tatcaagtgg tagatatgga
     1381 tgcagcccaa gaaaataggt ttgtttggtt aaatatggag agtgaccata cacaatggat
     1441 aaaatgggca atagatgagg gtattgaaag aaaagttatc gagtttattt ccacttttcc
     1501 agaatatctg cataaaataa atgaagatga tgtaagagcc actcctagaa gttatgagag
     1561 agtatctaaa atttataaag tttataaaga aaaaaataat tctattccta gagcagtatt
     1621 tttaaatgtt gtaaagggta atgtaggtaa agttattgca gaagaattta taagttttat
     1681 tgaatcaaac tctgaaccat taatatccta tgaagatgtt tttttaggag aatctattga
     1741 tgagtctatt gtagaaagag taaaaaatga gagtcataca agactttatc tatcagctat
     1801 gaacattcta aaagacttag aattaaatat taagaatgat aaatatgaat caaaccacta
     1861 tattaataga tttattgaat ttttaaaaat gtatcctgta gatttaatga taggtattat
     1921 gaaggatatt agaaacagtt atattgaagt atataaaaaa gctatagaaa atgaagagtt
     1981 tgtaaaatca tattttgaat cttatagttt aataagggga taatgtatgg aaaattattt
     2041 tgataaacaa gcaaaagagc tttataataa agcaagtgaa attataaata ctcattctat
     2101 gctaaaggca aatcgtgcta atgagaaatt tgatatagat ataccacaag attttaagta
     2161 tgagtttttt agtcttgtag ataaagtgaa tttaagtctt atggaagaag aagataattt
     2221 ttatggatat tttttgtttc agatgtcaag agaaataaga tttgatataa gtagtcctac
     2281 aggtgtgaat tttaagggag ctaaatatat tatatatttt aatccaataa tctttttaac
     2341 gcttgacata aagcaaatgg agactacaat taaacatgaa attcatcata tattatctat
     2401 gcatttgatg agagcaaagg agttaaaagg aaagtatagt acattagcaa ttaatatggc
     2461 aatggatata gtagtaaata aatttctaaa caatttacca ccatatgcaa ctaccttaga
     2521 gtgggtaaat ttaaaatatt ccttaaaact tgaaccatat gaaccttttg aatattatgt
     2581 agaaaagatt cagactgaat tagatttgat ggaagtggat gaagatggtg aggaagatgg
     2641 tgaggaagat gatagtgata aatatggaga tatagaaatt gattatagcc ccgaaagaac
     2701 tcatgatata tgggatgaat ccaatgagat agatgaaaat acccttagag aatttactga
     2761 aaaatttatt aacagttctc aaaaaggtaa agttcctgct tatttagaaa gtatgatatc
     2821 atcacttaaa aatagtaagg gtgaattgcc atggaattta tatcttaata gaataatggg
     2881 aacagttgaa aataataaaa agaagactat aacaagaaga aatagaagac aaccaaatcg
     2941 tttagatttg aggggagaac ttagaagtca taagtcagaa atagcagttg cacttgatgt
     3001 aagtggaagt attagtgatg aagagtttaa acaagctatt aaagaagttc ttagtatagt
     3061 gaaaaactat aatcatgaaa ttacaattat agaatgtgat aatgaaatta gacgtgtata
     3121 taaagtgaga actgaaaaag atataaaaga tagaattagg ataggtggag gtactagatt
     3181 ttctccagtc tttgagtatg ctaataataa aaagattaat ttgttagtct actttactga
     3241 tggtaaaggt gaaaataaac tacaagtaat acctagaggg tacaaaattt tatgggttat
     3301 ttctggaaga ggagataaaa tttcattaaa agaaccttat ggagcagtta aaaaacttac
     3361 taaggttaaa acaaatgatg aaatattaga aataaatgat gtaagaaatg atgggtactc
     3421 aatgaatagc caagcaccaa ttttataggt attcacagga gataagctgt attaatttat
     3481 tttgatatgc ttgttataaa aatctaaaaa atacattaat caatggttaa tgtatttttt
     3541 aattttaata atataagttt tacttacatt taagtctcaa aatgtgttaa aatataatat
     3601 atagcagata gttttggcaa ggtggattta gttgtcaaaa ctattttttt tattaaggat
     3661 ggtgatgttt ttgtttaaga ttgatgatta tataatgtat ggtatgacag gagtctgcaa
     3721 agtcatggat ataacaaatg aacgatttac aaatggaata aaaaaagagt attatgtatt
     3781 gagtcctatt tattctaata atacagtaat aaaaattcct gtagataatg aaaaagtacc
     3841 aatgagaaag ttactttcta aaattaatgt gctttcatta ataaatgata ttcctaatat
     3901 ggatacatca tggatagata atgaaaaatt gagaagtgag caatttaaaa aaatactcag
     3961 aggtggtaaa tgtgaagagt taataaaatt agtaagaagt atagataata acagagagta
     4021 tgtaaaatct attggtaaaa aaacacatca agcagatgat aatataatga aagaagctga
     4081 aaggttatta aatgaagaat ttgcaactat tttagatatt tctcctaatg aagttagttc
     4141 atatatttca agtcatatac ctcaataaag tgttttagta tcctttattt aatctatttt
     4201 aagtattatt tatgtatgct ggttttggta aaataaaata tataattaaa ttatataaaa
     4261 aataataatg attgtattga agaattacag aatagtgcgg agagcctact cctttaggtg
     4321 tgagatggat atcacttaaa gatttaaatt gttattgtag atgtttttaa tataaaacaa
     4381 gattaataag tgtattgtaa gctttaagtg aaattgatac tataatgagt tacttagatt
     4441 ttaaaatata taatattata aaaaatcctt ttaggttttg aatgaataag atggatacta
     4501 tgttgaatag aatccatctt atttaagttt tatttatgtt gtttatttgt aagcattatt
     4561 tttgtacatg aaaacacagt gtaaaaatat gcttagaatt atgaagtttt attaaaaaga
     4621 tattcgacaa aataagacta aaaatattct taaacttata actaagataa atattgtata
     4681 tttatagtgt agcctgaaaa attgtggatg tacgaaattt atttagtatt ttattgaaac
     4741 aaagtagaag aagtactata gtttatcttt gaatagattg ttatttttta gtgaaaaagt
     4801 tattaggtta atacataaat ataatagctt ataaatagtt taagcatgtt aaaaaagtag
     4861 ttattagaga tatatagtta cattgcttat aagatactta aaatgatagc attcacttat
     4921 attcagaaga ttaagataaa aaatcatttt tatatagtaa agcataccta aatttagaaa
     4981 ataattgaat ttataaaatt aattaaatac ataaaaaagt attcaatcaa cttaacaaat
     5041 tttcttaaat ttaatttaaa ttttttattc tattaattat taatattatt tt
//
LOCUS       DAELLV010000241         5058 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.241, whole genome shotgun sequence.
ACCESSION   DAELLV010000241 DAELLV010000000
VERSION     DAELLV010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5058
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.241"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            180..1331
                     /locus_tag="KPD58_003200"
     CDS             180..1331
                     /locus_tag="KPD58_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBG0266931.1"
                     /translation="MEIYLDNSATTKPYQEVIDKMVYALNTEYGNPSSVHRKGVEVEK
                     AIKEVRQDIAKSLGAKEKEIYFTSGGTECNNTIIRGITSLNKKRKNHIISTNIEHPSV
                     LNTLKDLEEDGFEVTYLEVGKDGKINIEDLKNAIKSTTCLVSMMHVNNEIGTIQPIGE
                     VGKYLKGLKEKIYFHVDAIQSYGKINFRPSKYNIDFMSVSAHKFHGPKGIGFMYIKEN
                     NRLKPMLTGGGQEIGIRSGTENVPGIYGLGEAVRILNKDLDAVISKVNNLKNILKNEI
                     IDNIEDIKINSPEDGVCHILNVSFRGTKGEVLLHYLEQKGIYVSTGSACSSKKKGSYV
                     LNAIGLTNEEINGTIRFSLSDMNTEEEMLEAVKVLKESICDLRSIMKRK"
     gene            1377..2552
                     /gene="thiI"
                     /locus_tag="KPD58_003201"
     CDS             1377..2552
                     /gene="thiI"
                     /locus_tag="KPD58_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBG0266932.1"
                     /translation="MVYNILIVKYGEIGVKGKNRYIFENRLIRNIRNMLKPIGRFNVY
                     KEYGRIYVDLEDYDYEEVIEEVRKVFGIVGVCPGVRAKKDYDTLKEIALKMLEEKIEA
                     GYKTFKVESRRGDKSFGLTSQEMSMDIGGYLLSKVGDRINVDVRNPEVKIKCEYREFH
                     TMVYSDTIPGYGGLPLGTNGRAMSLLSGGIDSPVATWMVAKRGMEVEAVHFHSYPFTS
                     ERSQEKVKDLAKILAKYCGRVRLHKVNILEIQKAIGENCNEEEATILSRRFMMRIAQR
                     LSEKRHCDALITGESIGQVASQTIQGLTCTNAVVDLPVFRPLIAMDKSDIVDIAKKIG
                     TFETSILPEEDCCSVFSPRKPVTKPRLEKIEKSETALDIEKLVQDAIDKIEVEDIEF"
     gene            2708..3511
                     /locus_tag="KPD58_003202"
     CDS             2708..3511
                     /locus_tag="KPD58_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG0266933.1"
                     /translation="MNKKGFTLIELLVVISIIGILVIVAVPALFRNIEKSKAVTCLSN
                     RENIKTQIVIAMAEESSKGKNEVMKEVLENKDGKYFETEPKCKSGGIYSATFDDGYDG
                     ITGIESIAKVYVTCTKHPDGVEMARDIHQSMKDLIASFAQDPSIIPGASKGNDDFRKY
                     LLDNKYKNGWPTIPDEFKAKYGLSKDTLYIQPYAYSPTKSDATVVVFANNKTGGNWYT
                     SLVYDYDEGRWYKGKNGISVAGRSWDVDTDSVKSVKTEIHSKEGWGPLN"
     gene            3754..4395
                     /locus_tag="KPD58_003203"
     CDS             3754..4395
                     /locus_tag="KPD58_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HBG0266934.1"
                     /translation="MNNNFQDNQTINLIQSRRSIRKFTTEQISDEQVNTLLHCAFAAP
                     SGCNKQPWHITVVQDQKLLKEISDDTLSRIHEVSNVEINKNFKLFYGAPTVLFISYDE
                     SSSWAPYDIGILTGNITTAAQALGLGSCIIGMVRGLFTPVEQGDIEGLVSVLDKEDVK
                     ESESIKMKFDTNKKYRELLDIPEGYSVPFGIAVGIPDGNLPNAREVVYKVSRV"
BASE COUNT         2030 a          472 c          949 g         1607 t
ORIGIN      
        1 tataagtata ttatatttta ctataaaaat ttgtaataaa gtgaaaaaac tagcttttca
       61 tgaaataata ttttataatt taagattgtc tttttaataa agattaacaa aaaaaatact
      121 ataattagtg gtataatagt tagagttgaa gattttaaat aaaggtaggt gtaattaagt
      181 tggaaatata cttagacaat agcgcaacta caaaacctta tcaagaagtt atagataaaa
      241 tggtttatgc acttaatacg gaatatggaa atccttcttc tgtccataga aaaggtgtag
      301 aagttgaaaa agctataaaa gaagtaagac aagatatagc taaatctcta ggagcgaaag
      361 aaaaggagat ttattttaca tcaggtggta cagagtgtaa taatactata ataagaggta
      421 ttactagttt aaataaaaaa agaaaaaatc acataatatc tactaacata gaacatcctt
      481 ctgttttaaa tacattaaaa gatttagaag aagatggttt tgaagtaact tatttagagg
      541 tcggaaaaga tgggaaaata aatatagagg atttaaaaaa tgcgataaag tcaactacat
      601 gcttagtaag catgatgcat gttaacaatg aaataggaac tatacagcca ataggtgaag
      661 ttggaaagta tttgaagggt ttaaaagaaa aaatttattt ccatgttgat gctattcagt
      721 catatggaaa aattaacttt agaccatcta aatacaatat agattttatg agtgtaagtg
      781 cacataaatt tcatggtcca aagggaattg gatttatgta tataaaagaa aataatcgcc
      841 ttaagcctat gttaacaggt ggtggtcagg aaattggaat taggtcggga acagaaaatg
      901 tacctggaat atatggtctt ggagaagctg ttagaatact aaacaaagat ttagatgctg
      961 ttatttcgaa agtaaataac ttaaaaaata tattaaaaaa tgaaataatt gataatatag
     1021 aagatataaa aattaattct ccagaagatg gagtatgtca tatattaaat gtatcattta
     1081 gagggacaaa aggagaagtt ttacttcatt acttagagca aaaaggtatt tatgtatcaa
     1141 ctggctctgc atgttcttct aaaaagaaag gtagttacgt attaaatgct ataggtctta
     1201 caaatgaaga aataaatgga acaataagat ttagtttatc tgatatgaat acagaagaag
     1261 aaatgttaga agctgtgaaa gttttgaaag aatcaatttg tgacttaagg tcaataatga
     1321 aaagaaaata aagaatactg tatagatagt gataataaat tagtttaaga ggtgaaatgg
     1381 tgtataatat attaatagta aagtatggag aaattggagt aaaaggaaag aatagatata
     1441 tatttgagaa taggcttata agaaatataa gaaatatgtt aaaaccaatt ggaagattta
     1501 atgtatataa agagtatgga agaatatatg ttgatttaga agactatgat tatgaagaag
     1561 taattgaaga agttagaaaa gtatttggga tagtgggtgt gtgccctgga gtaagagcta
     1621 aaaaagatta tgatacatta aaagaaatag ctttaaaaat gttagaagaa aaaatagagg
     1681 ctggatataa aacttttaag gtagagtcaa gaagaggaga taagtctttc gggttgactt
     1741 ctcaagaaat gagtatggat ataggtggat acttattatc aaaggtagga gataggatta
     1801 atgttgatgt aagaaatcca gaagttaaaa taaagtgcga atacagagaa tttcatacta
     1861 tggtctatag tgatactata cctggatatg gtggattgcc acttggaacg aatggtagag
     1921 ctatgtctct tttatcagga ggtatagata gcccagtagc tacatggatg gttgcaaaaa
     1981 gaggtatgga agtagaggca gttcactttc atagttatcc atttacaagt gaaaggtcac
     2041 aggaaaaagt taaggattta gctaaaatac tggcaaaata ctgtggtaga gtaaggcttc
     2101 ataaagttaa tatcttagaa atacaaaaag caattggcga gaattgtaat gaagaagaag
     2161 ctactattct ttcaagaaga tttatgatga ggatagcaca gagactttct gaaaaaagac
     2221 attgtgatgc tttaattaca ggtgaaagta ttgggcaggt tgcatctcaa actatacaag
     2281 gacttacttg tactaatgcc gttgtagact taccagtgtt tagaccactt atagcaatgg
     2341 ataaatcaga tatagttgat atagccaaaa aaatagggac ttttgaaaca tctatattgc
     2401 ctgaagaaga ttgttgtagt gtattttctc ctagaaagcc cgtcactaaa cctagattag
     2461 agaaaataga aaaatctgaa acagcacttg acattgaaaa attagtacag gatgctattg
     2521 acaaaataga ggtagaggat attgagtttt agcatacaag ttatttaatt actaaattga
     2581 atattgagta tattagtagt tctgtaagta tattagtagt tctgttgaaa ggatttatgt
     2641 agacaattta ttaatatgta gtatttaaaa ataagtacta taattttatt taatgggaat
     2701 gattattatg aataaaaagg gttttacact aattgaattg ttagtagtta tatctataat
     2761 aggcatttta gttatagtag ctgttccagc attgtttaga aatatagaaa aaagtaaagc
     2821 agttacatgt ctttctaata gagaaaatat aaagactcaa attgttattg caatggctga
     2881 ggaatcaagt aaaggcaaga atgaagtcat gaaagaggta ttagaaaaca aagatggtaa
     2941 gtactttgaa acagaaccaa agtgtaagtc aggtggaata tattcagcaa cgtttgatga
     3001 tggttatgat ggaataactg gaatagaaag cattgcaaaa gtgtatgtta cttgtacaaa
     3061 acatccagac ggtgttgaaa tggctaggga tatacatcaa agtatgaaag atttgattgc
     3121 atcatttgca caagaccctt ctataatacc aggagcttca aagggtaatg atgattttag
     3181 aaaatattta ttagacaata aatataaaaa tgggtggcct acaattccag atgaatttaa
     3241 ggcaaaatat ggattaagta aggatacact atatatacaa ccatatgcat atagtcctac
     3301 taaatctgat gctactgtag ttgtatttgc aaataataag actgggggta attggtatac
     3361 ttccctagtt tacgattatg atgaaggtag atggtataaa ggtaaaaatg gtatttctgt
     3421 tgcaggtagg tcatgggatg ttgacacaga tagtgttaag tctgtaaaaa cagagattca
     3481 ttctaaagag ggatggggtc ctttaaatta atatatcatg ttttattaat ctggtattga
     3541 ttaaactata gaaaagagca gtttctatag atgacgaata ccagattttc atttatatat
     3601 ataattgaga tatattaaaa gtgtttatat ttttaagtct tcacatacaa gctaaaaata
     3661 attataataa tattgtaaag aaatttaatt ataagattaa ttttctgaag atttagtatt
     3721 tgtactaaga aatacataaa aaggggaata gatatgaata ataattttca agacaaccaa
     3781 acaataaatt taatacaaag tcgtagaagt atacgaaaat ttacgacaga acaaattagt
     3841 gatgagcaag taaacacatt attacactgt gcatttgcag caccttcagg atgtaataaa
     3901 cagccttggc acataactgt tgtgcaagac cagaaacttt taaaagagat tagtgatgat
     3961 actttatcta ggattcatga ggtgagtaat gttgagataa ataaaaactt taagttattt
     4021 tatggtgctc caaccgtttt atttatttct tatgatgaaa gtagttcatg ggctccatat
     4081 gatataggaa tacttacagg gaatataaca acagctgcac aagcactagg gcttggaagt
     4141 tgtataatag gaatggttag aggtttattt acacctgttg aacaagggga tattgaaggt
     4201 cttgtgagtg tattggataa agaagatgta aaagaatcag agtcaataaa gatgaagttt
     4261 gatacaaata aaaaatacag agaactactt gatataccag aagggtattc tgtaccattt
     4321 ggtattgctg taggtattcc agatggaaat ttaccaaatg ctagagaggt agtttacaaa
     4381 gtttccagag tttaggataa agtattgcaa gaaccaatca gttacttgaa gaattaaacc
     4441 agtaatatac aaataaattg atggaagagg aagctattta aaaagtagtt tagatttttc
     4501 gttgtaaaat aattaaataa agaataaggt gataagtatt ttatttatta tcttattctt
     4561 ttttattttt gttttgtaga atgataaaaa taaggattaa gtgtaataaa atagaaaaca
     4621 agtatgttaa ttttaagtat atgatttaaa atttatccaa gttaaaaatt taagatatct
     4681 tttcagtata atggaaaaaa tgtcaaaata ttgaaatagg gaaaaaatag tgatataata
     4741 agaaaaaata gagaaaaaaa gtaaccctta gttataaaaa agttactatt tattgaaaat
     4801 ttagatattt ttctaaaaat tatctatgtt aatagttaaa tttatgaaca ttttttgttt
     4861 ttatgttagg atatttctta gaaatgaaat acttcttaat ttgatttgat atgtatattt
     4921 ttatagctat tttattataa aaaaaaagaa cccttttata acaaatggaa actatggatg
     4981 taataattaa aataaaaaat attattttat atttataaac attgacattc taattttgta
     5041 gtgtaatcgt atttttta
//
LOCUS       DAELLV010000242         5026 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.242, whole genome shotgun sequence.
ACCESSION   DAELLV010000242 DAELLV010000000
VERSION     DAELLV010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5026
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.242"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            77..1480
                     /locus_tag="KPD58_003204"
     CDS             77..1480
                     /locus_tag="KPD58_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0266935.1"
                     /translation="MHKPSYKTVFHSYIIFFMIFICLILTVIGIFLSLITIQKPNGEF
                     IRSDWPKKFTENFNEQIVFIDNKPQVKQSGLEKLRENQLWLQILDSSGNRVYGFLEPK
                     EHKDYYSNSELLALANQNNSSNGTVYFNTIKNNKNDYIYIIYFPLNISKVNMFFDGGK
                     FIGGKSIVFSIIGIIFLIILVSGIAYGYWVTKCISNITSAVSDISKRSYLPVNIKGVF
                     ADIYNSLNILNEEIHKSDEIQNKTDIMREEWISNITHDLKTPLSPIKGYAELLTDSST
                     NYSPEKVYKYASIMLKNIEYTNTLINDLKLTYQLKNGIIPLNKNNYDLVRFLKELSID
                     ILNYPEYESKIVEFKSNIDNINFDFDCTLLKRAFSNLIVNAFTYGYKNTKVCVHITLN
                     ERIEIIISDNGKGMSREEASNLFNRYYRGSNTKQKIEGTGLGLAITKQIIEVHGGNIS
                     VESIPGLGSSFYITFPI"
     gene            1699..4176
                     /locus_tag="KPD58_003205"
     CDS             1699..4176
                     /locus_tag="KPD58_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG0266936.1"
                     /translation="MKIILKYIFTNIKERKIRTAVMLLSIVLSTVLLFVSFSIGLSYE
                     SAQRKMAKGMYGTATISVQSKNPDILTNLEDIPDLNTIKSKVGVLESSAIYNKGGYYE
                     EFSLISADLSQLNKINKPRLENGDSITDFSGDKIILPNRFTSKYKIKKGDSITLQIYG
                     KSYTFQVSDIASYDTVFLRNTRGVNALLPKETLSKIINKGSGYTRVLIESEEEITENL
                     VNKLSEELSTEKYMVSNTINETKIISDARQKTMPFFLISFFALTLSIFIIYSSYKVIT
                     LERLPFIGTFRSIGANEKTVTRILMLESILYGSIGGLIAIPIGVVVLNLMLHGLGNSL
                     EQGISIPVVISPIGVIISVIVAIIVSSFSAYIPVKKASHLPIKNIVLGTVEEKNVSNR
                     SILFIGSIMFILSILLPRISPENTLYLAGGFSLLGLIVATIVLIPLITDIMSIVFEFV
                     YKNILGNEGKLAARNMKNNKNIIQNITLLFISISAVIAISVVGNFVKTYITDVFRDAE
                     LQGFADGKMNEEFIEDVRHMDGIKKILPLYVMNNEISGNGVTLSRLEGTDNIKLYNSM
                     FGINYTNFEIKKQVIEAFNDKRSVILNEDTLKKVGLSIGDTITLSNDKYDFSYKIVGS
                     FKSRANDVEAVIPSHYAVNDFDKTNYGFLVYTAVNPDAVMIQIRYLFGDTYNWSRTVE
                     EFNNDSLNTISSFLSPMNKMTYFIFLLATVGIINNLLINYIQKRRSIAMYKSIGLSDK
                     QNIKVTLIEGFTSGLLGAVIGIVISILEIQTIFIVAGPKISMKPDLDFKTFIIVGLLG
                     IIVTLIGSIVPIIKGKKMKLIEEIKFE"
     gene            4191..4877
                     /locus_tag="KPD58_003206"
     CDS             4191..4877
                     /locus_tag="KPD58_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0266937.1"
                     /translation="MKITHNSLAVEAKNIIKEYKIGNTTTRVLKDVSLQVMKGEFVSI
                     MGQSGSGKSTLLYILGGLDTPTSGKVYMNGADISHFNDEKMSIIRRRNIGFVFQFYNL
                     IPNLNVEENIMLPLLLDGKNLKDYKNQLDEILDIVGLTDRRKHTPRELSGGQQQRVAI
                     ARALIGKPEILFADEPTGNLDSKTGIEIIDLLNKINRDNGQTIIMVTHSPEAAKSSSR
                     TIIVSDGLIV"
BASE COUNT         1939 a          533 c          796 g         1758 t
ORIGIN      
        1 catatcaata attattgtct attttatata agcaacaatc aaactaaaat aaaagtttaa
       61 gaaaagagtt gattaaatgc ataaacctag ttataaaaca gtatttcatt catacattat
      121 ttttttcatg atttttattt gcttaatcct gacagtaata ggtattttct tatcacttat
      181 tactattcag aaaccaaatg gtgaatttat tcgtagtgat tggccgaaaa aatttacaga
      241 aaactttaat gaacaaatcg tatttataga taataagcca caagtaaaac agtctggatt
      301 agaaaaactt cgagaaaatc aattgtggtt gcaaatttta gattctagtg gaaatagagt
      361 ttatgggttt ttagaaccaa aagagcataa agattattat tctaattctg aattattagc
      421 attagctaac caaaataatt caagtaatgg aactgtatac tttaatacaa ttaaaaataa
      481 taaaaatgac tatatttata ttatatattt tccattaaat atatctaaag tgaatatgtt
      541 ttttgatgga ggtaaattta taggtggtaa gtcaattgta ttttcaatca tagggattat
      601 atttttaata attttggttt ctggaattgc ttatggttac tgggtaacaa agtgcatatc
      661 aaatattaca tctgctgtta gcgatatttc aaaacgaagc tatcttccag ttaatattaa
      721 aggtgttttt gctgatatat ataatagttt aaatatttta aatgaagaaa ttcataaaag
      781 tgatgagata cagaataaaa ctgatattat gagagaagag tggatttcaa atataactca
      841 tgatttaaaa actccacttt caccaataaa agggtatgca gagcttctta cagatagtag
      901 tactaattat tcacctgaaa aagtatataa atatgcttca atcatgttaa agaacataga
      961 atatacaaat acacttataa acgatttaaa attaacctat caattaaaaa atggaataat
     1021 ccctttaaat aaaaataatt atgatttagt aagatttttg aaagaattat ccattgatat
     1081 tttaaattat ccagaatatg aatcaaaaat agttgaattt aagagtaata tagataatat
     1141 taactttgat tttgattgta cactattaaa acgagctttt agtaatctta ttgtcaatgc
     1201 ttttacatat ggatataaaa atactaaagt ttgtgtacat ataacattaa atgaaagaat
     1261 tgaaatcatt atatctgata atggaaaagg gatgtcaaga gaagaagcat caaatttgtt
     1321 taatcgttat tatagaggaa gcaatacaaa acaaaaaata gaaggaactg gtttaggact
     1381 agcaataacg aagcaaatta ttgaagttca tggagggaat atatctgttg aaagtatacc
     1441 tggattaggt agctcatttt atatcacctt tcctatttaa ttaaggttaa ttaaggtcaa
     1501 ataaaaaata tattaaggtt gatagattat tatatttata tagtagttta tcaatctttt
     1561 ttatttaagg ggtgatttga ttaaaaatat tataaatata atgataaaaa tatggaggag
     1621 tctattagag atttaattta gattaaagta taaaaatatt agattgaaat atcttagata
     1681 agggaagaag gtgtaagttt gaaaattata ttaaagtata ttttcacaaa tattaaagaa
     1741 cgaaaaatta gaacagcagt tatgttattg tccatagttt tatcaacagt tttacttttt
     1801 gtttcattct caattggatt atcttatgaa agtgcacaaa gaaaaatggc aaaaggtatg
     1861 tatggtacag cgactatatc tgtccaaagt aaaaatccag atatattaac caatttagaa
     1921 gacatacctg atttaaatac tataaaatca aaggttggtg tattggaaag ctctgctatt
     1981 tataataaag ggggatacta tgaagaattt agtcttatat cagcagactt aagtcaacta
     2041 aataaaatca acaagccacg attagaaaat ggcgatagta ttactgattt ttctggtgat
     2101 aaaattattc ttccaaatcg ctttacatca aaatataaaa taaaaaaagg tgattctatt
     2161 actttacaga tttatggaaa gtcctatact tttcaagtta gtgatattgc atcttatgat
     2221 acagtatttt tgaggaacac aagaggtgta aatgcactgc tacccaaaga gactctatca
     2281 aaaattataa ataaaggcag tggatataca agagttctta tagagtcaga agaagaaatt
     2341 acagaaaatc tagtaaataa attatccgaa gaactttcta ctgaaaaata tatggtatca
     2401 aatactataa atgaaactaa gataatttca gatgctagac aaaagacaat gcctttcttt
     2461 ttaataagtt tttttgcatt aacccttagt atatttataa tatacagcag ttataaggtt
     2521 attacattag aacgtcttcc ttttattgga acatttagaa gtattggtgc caatgaaaaa
     2581 actgtaactc gtatattgat gcttgaaagt atattatacg gaagtatagg aggattaatt
     2641 gctattccta ttggtgtagt tgttttaaat ttaatgcttc atgggttagg aaattctctc
     2701 gaacaaggaa tatcaattcc agtagtaata tcacccattg gagtgattat atctgttata
     2761 gtagcaataa tcgtttcttc atttagtgca tatatacctg ttaaaaaagc tagccatttg
     2821 ccgataaaaa atattgtact aggaacagta gaagaaaaga atgtttcaaa tcgttctata
     2881 ctttttatag gaagtataat gtttatttta tctattttac ttccaagaat atcccctgaa
     2941 aatacattgt atctagctgg tggattttca ttactgggat taattgtagc tactattgtg
     3001 ttgattcctc ttattactga tatcatgtct atagtttttg agtttgttta taaaaatatt
     3061 ttagggaatg agggtaaatt agctgctaga aatatgaaaa ataacaaaaa tataatacag
     3121 aatatcactt tactatttat aagtatttct gcagttattg caataagtgt tgttggtaac
     3181 tttgtcaaaa cttatataac agatgtgttt cgtgacgcag aattacaagg ttttgcagat
     3241 ggaaagatga atgaagagtt tattgaagat gttagacata tggatggaat taaaaaaata
     3301 ttacctcttt atgttatgaa taatgagatt tctggtaatg gtgtaacatt atcaagacta
     3361 gagggtacag ataatataaa actatataac tctatgtttg gaatcaatta tacaaatttt
     3421 gagataaaaa aacaagtaat agaagctttt aatgataaac gttctgttat tttgaatgaa
     3481 gatactctaa aaaaagtagg cttatctata ggagatacaa taactctatc taatgataag
     3541 tatgattttt cttataaaat agtaggtagt tttaaatccc gtgcaaatga tgtagaagct
     3601 gttataccat cacattatgc agttaatgat tttgataaaa ctaattatgg ttttttagta
     3661 tatacagcag tcaatccaga tgctgttatg attcagataa gatatttatt tggagataca
     3721 tataattgga gtcgtacagt tgaagaattt aataatgatt ctttaaatac tataagtagt
     3781 tttttgtctc ctatgaataa gatgacctat ttcatttttc ttcttgctac agtaggtatt
     3841 attaataatc tactgattaa ttatattcaa aaaagacgtt ctatagctat gtataaatct
     3901 ataggactaa gtgataaaca aaatataaaa gtaacattga ttgaagggtt tacttcaggt
     3961 ttacttggag ctgttattgg tattgtgata tctatacttg agattcaaac tatctttata
     4021 gttgcaggac caaaaatttc aatgaaacca gatttagatt ttaaaacatt tattattgtt
     4081 gggttattgg gaattatagt aactttaata ggttcgatag tgcctattat aaaagggaag
     4141 aaaatgaaac tgatagaaga aattaagttt gaataattat ggaggttaat atgaaaatta
     4201 cacataatag tttagctgta gaagcaaaaa atattatcaa agaatataaa attggaaata
     4261 caacaactcg agtgttaaaa gatgtatctt tacaagtaat gaaaggtgaa tttgtttcta
     4321 tcatgggtca atctggttct ggcaaaagta ctcttttata tattttaggt ggtttagata
     4381 ctccaacgag cggaaaagtg tatatgaatg gagctgatat atcacatttt aatgatgaga
     4441 aaatgagcat aatacgtcgt agaaatatag gattcgtatt tcaattttat aacttaatac
     4501 caaatttaaa tgtggaggaa aatataatgc ttcctttgtt attagatggt aaaaatttaa
     4561 aagattataa aaatcaatta gatgaaatct tagatatagt tggtttaact gatagacgta
     4621 aacatacacc acgagagtta tctggtggtc agcaacaacg tgttgctata gctcgagctt
     4681 taattggaaa accagagatt ttatttgctg atgagcctac tggaaattta gatagtaaaa
     4741 cagggataga aataatagat ttactgaata aaataaacag agacaatgga caaactataa
     4801 ttatggtaac tcactcacca gaagcagcca aaagtagtag tcgtactatt atagtttcag
     4861 atggtttaat tgtataagga ctaattattg tatttgagat aagcattttt aattatataa
     4921 attagacata tgtaaagtgg atataagtta aatactataa gtgtgctata atattaaaat
     4981 aacagataaa agtactatat tttttattga ttattttaat tttatt
//
LOCUS       DAELLV010000243         4985 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.243, whole genome shotgun sequence.
ACCESSION   DAELLV010000243 DAELLV010000000
VERSION     DAELLV010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4985
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.243"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..266)
                     /locus_tag="KPD58_003207"
     CDS             complement(<1..266)
                     /locus_tag="KPD58_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBG0266938.1"
                     /translation="MILKKHRETILYLFFGAFTTLVNIVSYLFFTRVILFNFMVANAL
                     AWILAVLFAYVTNKFFVFESKRIELIFLFKEFLFFVSFRLLSGI"
     gene            493..2904
                     /locus_tag="KPD58_003208"
     CDS             493..2904
                     /locus_tag="KPD58_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp84"
                     /protein_id="HBG0266939.1"
                     /translation="MRKYKSKNLSKLLALLTVCFLIVSTIPVSAENHKTLDGVETAEY
                     SESYLQYLEDVKNGDTAKYNGVIPFPHEMEGTTLRNKGRSSLPSAYKSSVAYNPMNLG
                     LTTPAKNQGSLNTCWSFSGMSTLEAYLKLKGYGTYDLSEEHLRWWATGGKYGWNLDDM
                     SGSSNVTAIGYLTAWAGPKLEKDIPYNLKSEAQGATKPSNMDTAPTQFNVTDVVRLNK
                     DKETVKNAIMQYGSVTSGYAHYSTYFNKDETAYNCTNKRAPLNHAVAIVGWDDNYSKD
                     NFASDVKPESNGAWLVKSSWGEFNSMKGFFWISYEDKTLLTDTDNYAMKSVSKPDSDK
                     KMYQLEYAGLSKIMSNKVTAANVFDFSRDSEKLDSVMFETDSVGAKYEVYYAPVVNGV
                     PQNNSMTKLASGTVSYSGYINVPTNSYSLPKGKGAIVVVIDNTANPNREKSTLAYETN
                     IDAYYLYEAKANLGESYILQNNKFEDINTYSEFSPCNFVIKAITKTSSGQATSGESLT
                     GADRYETAVKVSQKGWTSSQNAVLVNGDAIVDALTATPFTAAIDSPILLTGKDNLDSK
                     TKAELQRLGTKKVYLIGGENSLSKNVQTQLSNMGISVERISGSDRYKTSISLAQKLNS
                     IKSVSQVAVANGVNGLADAISVGAAAADNNMPIILTNEKSELQGADEFLNSSKITKSY
                     IIGGTATLSSNLESKLSNPTRLAGSNRNETNAKIIDKFYPSSDLKYAFVVKDGSKSQG
                     DLIDGLAVGALGAKTDSPVVLVGNKLDESQKNVLKSKKIETPIRVGGNGNENAFNELN
                     TLLGK"
     gene            3201..4778
                     /locus_tag="KPD58_003209"
     CDS             3201..4778
                     /locus_tag="KPD58_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp5"
                     /protein_id="HBG0266940.1"
                     /translation="MKAPKTILTILTIALTLSSISIIPSYALTEEKLIGNGRYETAVK
                     ISQKAYSSSPNVVLVNDNSLADALSATPFAKAKGAPILLTESDKLDDRTEKEIKRLGA
                     KDIYLIGGTAVLNKDIENKLKSNGLNVERINGKDRYETSLILANKLKDIKDIKEVAVV
                     NGEKGLSDAVSVGAPAAQNKMPIILSSPKDGVEAFDKFIRDEKVIKAYVIGGTNSVSR
                     AVEKSLPNAERVSGKDRNETNAKVIEKFYTDTNLSNLYVTKDGSKNENQLIDSLAVGV
                     LAAKNESPVVLVGNKLNTKQRDILSTKKLNTITQVGGNGNEEAFDEIKSLQEKTVFEA
                     KTVEELTDMINIASPNDIINFKPKENTVNEAFRMVTNKPITVNIKGDCSKTLTVDMPN
                     GEVNNYATLVNVIVRNIGEGGFNNHDTITILSVRDKNGRVIENTRNSDIDTLMILASA
                     NDTKLINDGYIGKLIDNSSNSDITNNGTIDKKVNQVEDLEAKVDSIEKAIDSISQKVN
                     KIQDILDKLGFLKKFLS"
BASE COUNT         2043 a          605 c          873 g         1464 t
ORIGIN      
        1 ataataccag aaagtagcct aaaactgaca aaaaacaaaa actccttaaa caaaaatatt
       61 agttcaattc ttttactctc aaacacaaaa aacttattag taacataagc aaataaaaca
      121 gcaagtatcc atgccaaagc atttgcaacc ataaaattaa ataaaataac tctagtaaaa
      181 aataaatacg aaactatatt tacaagagta gtaaatgctc caaaaaataa atataatatc
      241 gtctctctat gcttttttaa aatcaaatat aatcctccta agctaattta atataaatat
      301 acttaaagat actaacaaac attagtataa cacatctatt aataaatcaa tatatcttaa
      361 aatattataa aaataaaact ataatattct ataattaata tgtactcata atccatattt
      421 tatcaaaata aaatatgacc ttaatttaga aatctgatat aataaaaatt aaatacatat
      481 aagggggtaa acatgagaaa gtacaaatca aaaaacttgt caaagctact agcattgttg
      541 acagtttgct ttttaatagt gtcaacaata cctgtctcag cagaaaacca taaaactcta
      601 gatggagtag aaactgcaga gtattctgaa agctatcttc aatacctaga agatgtcaaa
      661 aatggagaca cagcaaaata taatggagta ataccttttc cacatgaaat ggaaggtaca
      721 acacttcgaa ataagggtag aagtagtctt ccatcagcat ataaatcaag tgtagcatac
      781 aacccaatga atcttggtct tactacacca gcaaaaaatc aaggaagtct taatacatgc
      841 tggtcttttt caggtatgtc aacgttagaa gcatatctta aattaaaagg atatggaacg
      901 tatgacttat cagaagaaca tttaagatgg tgggcaactg gtggaaaata tggatggaac
      961 ttagatgata tgtcagggag ttcaaatgta acagctatag gatatctgac agcatgggca
     1021 ggtcctaagt tagaaaagga cataccatat aatcttaaat ctgaggcaca aggtgcaact
     1081 aaaccttcaa atatggatac tgcacctact caatttaatg taacagatgt tgttcgtctt
     1141 aataaagata aggaaactgt aaagaatgct ataatgcaat atggttcagt gacatcaggt
     1201 tatgcacatt attcaacata ttttaataaa gatgaaacag catataactg tacaaataag
     1261 agagctccat taaatcacgc tgtagcgata gtaggatggg atgataatta ttcaaaagat
     1321 aattttgcat ctgatgtaaa accagaatca aatggagcat ggttagtaaa aagtagttgg
     1381 ggagagttta attctatgaa aggattcttc tggatttctt atgaagataa aactctacta
     1441 acagatacag ataactatgc aatgaaatca gtatcaaaac cagatagtga taaaaaaatg
     1501 taccaacttg aatatgctgg tcttagcaag ataatgtcaa ataaagtaac agcagcaaat
     1561 gtatttgatt ttagcagaga ctctgaaaaa cttgattctg ttatgtttga aacagactct
     1621 gtaggagcaa aatatgaagt atattatgca ccagtggtaa atggagttcc tcaaaacaat
     1681 tcaatgacaa aacttgcaag tggaacagta tcatattctg gatacataaa tgtacctact
     1741 aattcttaca gcttaccaaa aggtaaagga gcaatagtag tagttataga caacacagca
     1801 aatcctaata gagaaaaatc aactttagca tatgaaacta atatagatgc atattattta
     1861 tatgaggcta aagcaaactt aggtgaaagc tacatacttc aaaacaataa gtttgaggat
     1921 ataaatacat atagtgaatt ttctccttgt aactttgtta taaaagctat aacaaaaaca
     1981 tcttctggac aagctacttc aggagaatct ttaactggag cagatagata tgaaacagca
     2041 gttaaagtta gtcaaaaagg atggacttct tcacaaaatg cagtattagt aaatggagat
     2101 gcaatagtgg atgctttaac agctacacca tttacagcag caattgactc tccaattctt
     2161 ttgacaggaa aagataactt agattcaaaa actaaggcag agttacaaag attaggaact
     2221 aaaaaagttt atctaatagg tggagaaaat tctttaagca agaatgtaca aactcaactt
     2281 agtaatatgg gtatatcagt agaaagaatt tcaggtagtg atagatacaa gaccagtata
     2341 tctctagctc aaaagttaaa tagtataaaa tctgtttcac aagttgcagt ggcaaatggt
     2401 gtaaatggac ttgcagatgc aataagtgtt ggtgcagcag ctgctgataa caatatgcca
     2461 ataatactta ctaatgaaaa aagtgagttg caaggtgctg atgagttttt aaattcatct
     2521 aaaataacta agtcttatat aattggtgga acagctactt tatcatcaaa tttagaaagt
     2581 aagctttcaa atccaacaag acttgcaggc agtaatagaa atgaaactaa tgctaagata
     2641 atagataaat tctaccctag ctcagatttg aaatatgctt ttgttgttaa agatggttca
     2701 aagagtcaag gagacttgat agatggacta gcagtaggag cattgggtgc taaaactgat
     2761 tcaccagtag ttctagttgg aaataagtta gatgaaagtc aaaaaaatgt acttaagtct
     2821 aagaaaatag aaactcctat tagagttggt ggaaatggaa atgaaaatgc ttttaatgaa
     2881 ctaaatactc ttttaggaaa atagtgaatg gaaagatagt aaggatatac attttattat
     2941 taaaaaatgt tcaatagaag tttataaata taaaataaca aaaaggggtg tctcaaaatg
     3001 aactttttag ttcatgaggc actctttttt gttgttttat atttttatca tttaaataat
     3061 gaaaaatagg attgtctata tttttagata gttttcttat tattgatgaa aaatgtaata
     3121 attttaaact gtaaaatagt gtaaagttat tttataatat aaatatagta agattttaaa
     3181 caaaataagg gggaataata atgaaagcac caaaaactat tttaacaata ctgacaatag
     3241 cgcttacttt aagtagcatt tctataatac catcatatgc acttacagag gaaaaattaa
     3301 taggtaatgg tagatatgaa actgctgtaa agataagtca aaaggcatac agttctagtc
     3361 ctaatgtagt gctagttaat gacaattcat tagctgatgc actttcagct acaccatttg
     3421 caaaggctaa aggagcacca atacttttga cagaaagtga taagctagat gataggactg
     3481 aaaaagagat aaaacgtcta ggagctaaag atatttattt gataggaggt actgctgtac
     3541 ttaataaaga tatagagaac aagttaaaaa gtaatggact taatgtagaa agaattaatg
     3601 gtaaggatag atatgaaact tcactgattt tggctaataa gcttaaggat ataaaagata
     3661 ttaaggaagt tgctgtagta aatggtgaaa aaggattgag tgatgctgtt agcgttgggg
     3721 caccagcagc tcaaaataag atgcctataa ttttgtctag tcctaaggat ggagtagaag
     3781 catttgataa atttataagg gatgagaaag ttataaaagc atatgtaatt ggaggtacaa
     3841 attctgtttc aagagctgta gaaaagagtc ttcctaatgc agaaagagtg agtgggaaag
     3901 atagaaatga gactaatgct aaagttatag aaaagtttta cactgataca aatttaagta
     3961 atttatatgt aactaaggat ggtagtaaaa atgagaatca actaatagac tcgttagctg
     4021 ttggcgtatt ggctgcaaaa aatgagtctc ctgttgtttt ggttggtaat aaattaaaca
     4081 caaaacaaag agatatactt agtactaaaa aattaaatac tataacacag gtaggtggaa
     4141 atggaaatga agaagctttt gatgaaatta aaagtctaca agaaaaaact gtgtttgaag
     4201 caaaaactgt tgaggaatta actgatatga taaatatagc aagtcctaat gatattataa
     4261 actttaaacc aaaagagaat actgtaaatg aagcttttag aatggtaaca aataaaccaa
     4321 taactgtgaa tataaagggt gattgttcaa agactcttac agtagatatg cctaatggtg
     4381 aagtgaataa ctatgctact ttggtaaatg ttatagttag aaatattgga gaaggtggat
     4441 ttaataatca tgatacaatc actatattgt cagttcgtga caaaaatggt agagttatag
     4501 aaaatactag aaattcagat atagatacgt tgatgatatt agcttctgct aatgatacaa
     4561 aattgattaa tgatggatat ataggaaaac ttatagataa ttcatctaat agtgatataa
     4621 ctaataatgg tactatagat aaaaaagtta atcaggttga agatttagag gctaaagtag
     4681 attcaattga aaaagctata gattctataa gtcaaaaagt caataaaata caagatatac
     4741 tagataagct tggatttcta aagaaatttc ttagctaaga atatgtatat ttaagtgaag
     4801 tgtgtgttgt aattgttcta aagttatgta gttaaacagg tttatataca aatagattta
     4861 tagataaata tgtagtttag tatatatttg aataagcttt ggggaggtaa catggggatt
     4921 ggaattgaat gtaacaaaaa aataaataaa agtattgaca aaactagaat aaagaaaaat
     4981 aatct
//
LOCUS       DAELLV010000244         4963 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.244, whole genome shotgun sequence.
ACCESSION   DAELLV010000244 DAELLV010000000
VERSION     DAELLV010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4963
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.244"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            477..1337
                     /locus_tag="KPD58_003210"
     CDS             477..1337
                     /locus_tag="KPD58_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG0266941.1"
                     /translation="MKIIQILNNNVALVKRGKNEIIVFVKGIGFKKRVGQVINENEIE
                     RTYILDSYDMLEHFSYLLQNSDSNNIVLINEIIEYGEKALNIKSSDYLSLTLLDHLEF
                     LLKRCEKNQFIKSPLVWNVKRFYPKHFEIGMHALKLIGDSKGLNLPDDEAVSIALHFV
                     NMEVNKESHDSTIVELRTLADIVSIIKYHFNVELDETSTNYMRFTTHLQYFIQRIMSD
                     EASIDEDNSIDLYKQVSQLYPKSFGSVQKIRKYIKSQFNVDISISEETYLMLHINRVT
                     QRLEAKSDEI"
     gene            1327..3198
                     /locus_tag="KPD58_003211"
     CDS             1327..3198
                     /locus_tag="KPD58_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG0266942.1"
                     /translation="MKYKDLNENIIKLIGGKENIKSVAHCVTRLRFTLNDRSIAQTEE
                     IKKLKGVIDVVSNDVAYQIIIGTHVVDVYNEFMSMIGLSSSESSIKSERKKGIRGIKG
                     IFNSIFVVVSETMTSVIEVLLAAGILASMLALLNMSGILQSDSPTYQILDTLRSATFH
                     FLPVLIAIASAKRLNVNPYIAVVLAVTLLSSSIDGVEGLSLFGINLQATTYANSFIPI
                     LLGVWAMGIIIEGLKKILPKALHYFLVPVLSLIITLSITLTVFGPIGMWIGDGLNYAC
                     MFLIDTLGNWSVVALYAALQPLLIVFGAANFTMPISLNSVTTLGYDPIFMGAATISDI
                     AVCGAMFGYFLKTRNKEQKQLFGTVSFSALMGVTEPSVFGVFMKYRRPFVAVMIGGGI
                     GGLIAGLAQVKTYGMVWGLAALPTYLVGGEVSNFTFMIISVVVSFVAATAASYLLGIP
                     SDEKEEVKGDNEKELELIINENNSRTISIGNVAKGQVIALGEVNDKAFSSGALGKGVG
                     VIPSESKSTVISPVDGTITIVFPTKHAYGIVTDEGLEILIHIGIDTVNLDGKFFESLV
                     TQNQRVKKGDALAVFESDKIIEEGFDSTIITVVTNTSDYLDVISSNDESEELLTVIV"
     gene            3218..4672
                     /locus_tag="KPD58_003212"
     CDS             3218..4672
                     /locus_tag="KPD58_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBG0266943.1"
                     /translation="MNIKDTFFWGGSIAAHQCEGSWDSDNKGPAIMDFVTKGSYETPR
                     VITDKIEEKLDYPSHNGIDFYNRYKEDIALFKEMGFSALRISIDWSRIFPNGDDENPN
                     ELGIKYYEGLIDTLIENNIEPIVTLYHFELPMNLVHKYGSWNNRKLIDLYLKYSETVI
                     RRFDDKVKYWVTFNEMNHIDPQTEHSDIFTYMIAGIKYSEIENKVQTLANVGYNMTLA
                     GVKVAKLAREINPNNKVGCVFGLNPIYPYNCNPSNVLNAFLDNDRDYYQIDAMCNGKF
                     PQYKLKEYEKNNITIEVTDEDKKAFKEGKLDFIGLNYYFSSVSTPKENLSGDKSLFGG
                     IQNPYLEQSKWGWAIDPIGLRFTLNYIYRKYQLPILITENGLGAYDKIEEDGTINDDY
                     RIEYLSKHLSQMKSAIEEDNVECFGYLMWGPIDLVSATTGQMSKRYGFIYVDLDDEGK
                     GSFKRYKKKSFDWYKEVIKSNGEVLDFSTDIVVG"
BASE COUNT         1911 a          521 c          905 g         1626 t
ORIGIN      
        1 actaaatttg aagctttacc tattaatata tcttaatagt gattaaataa aactaaactt
       61 aagactttat atgttaatat atcttaagaa tttatttgtt aacatacagt tcattatatt
      121 taaaagcatt actatgataa ttcataatag taaaatagat aatggttcag acaatattat
      181 aaatattagt gacggtaaaa tagtatcaat ttagtatata aaccttgtca gataatcgca
      241 taacagagct tgtagttata gaataaaaaa atatctagga aaccgttgac agatttatat
      301 ggatagtata acatatagaa tatgaaatta aacaaaaaag aattttaatt atggcgacgt
      361 agtcataatt ttgagtatgt tactgattag caggcagaac tcaagtatat atgtgaaaaa
      421 tagagggata ctctatacat atatgcttga gttcttttta ttgaagggag gaatatatga
      481 aaataattca gattttaaat aataatgtag ccttagtaaa aagaggtaaa aatgaaatta
      541 ttgtatttgt taaaggaatt ggctttaaaa aaagagtggg acaagtaata aatgaaaatg
      601 aaatagagag aacatatata ctagattcat acgatatgct agaacacttt agttatctac
      661 tacaaaactc agactccaat aatatagttc ttattaatga aataattgaa tatggtgaga
      721 aagctttaaa tataaagtca agtgattatc taagcttaac cttactagac catttagagt
      781 ttttacttaa aagatgtgaa aagaatcaat ttataaaaag tcctttggta tggaatgtta
      841 aaagatttta tccaaagcat tttgaaattg gaatgcatgc actaaaatta attggtgata
      901 gtaagggact taatcttcct gatgatgagg cagtatcaat agcacttcat tttgtaaata
      961 tggaggtaaa taaagagtct catgatagta caattgtaga gttgagaact ttagcagata
     1021 tagtttctat aataaaatat cattttaatg tagagctaga tgaaacttca acaaattata
     1081 tgagatttac tactcatcta cagtacttta tccaaagaat tatgtcggat gaagcttcaa
     1141 tagatgaaga taattctata gatttatata aacaagtaag tcaactttat ccaaagtcat
     1201 ttggaagtgt tcaaaagata agaaaatata taaaatcaca atttaatgtc gatatatcta
     1261 taagtgaaga aacttattta atgttgcata taaatagggt gactcaaaga ctggaggcaa
     1321 aatcagatga aatataaaga tttaaatgaa aatataataa aacttatagg gggaaaagaa
     1381 aatataaagt cggtagcgca ttgtgttact cgtttaagat ttactttaaa tgataggagt
     1441 atcgctcaaa cagaagaaat taaaaaatta aaaggagtaa ttgatgtagt ttctaatgat
     1501 gttgcttatc aaattataat aggtactcat gttgtagatg tatataacga gtttatgtct
     1561 atgataggtt taagttcaag tgaatcaagt ataaaaagtg aaagaaaaaa gggaattaga
     1621 ggtatcaaag gtatatttaa ttccatcttt gtggtagtct cagagactat gacttcagta
     1681 attgaagtac tacttgcagc tggtatactt gcaagtatgt tagcactttt aaatatgtct
     1741 ggcatacttc agagtgacag tcctacatat caaatattag atacattaag aagtgctaca
     1801 tttcacttct taccagtatt aattgcaata gcatcagcaa aaagacttaa tgtaaatccc
     1861 tatatagcag tagtgttagc agttacgtta ttatcttcat caatagatgg tgtagaagga
     1921 ttaagtttat ttggtataaa tttacaggct acaacttatg caaacagttt tattccaata
     1981 cttttaggag tatgggcaat gggaattata attgaaggtt tgaaaaaaat attacctaaa
     2041 gcattacact attttttagt tccagtatta tctttaataa taactctatc aattacatta
     2101 actgtatttg gacctattgg tatgtggatt ggtgatggat taaactatgc atgtatgttt
     2161 ttaattgata cattgggtaa ttggagtgtt gttgcactgt atgcagcttt acaaccactc
     2221 ttaatagtct ttggtgcagc aaactttact atgcctatat ctttaaattc tgtgacaaca
     2281 ttggggtatg acccaatttt tatgggcgct gcaacaatat ctgatattgc agtttgtggt
     2341 gcaatgtttg gatatttctt aaaaactagg aataaagaac aaaaacaatt atttggtaca
     2401 gtaagtttta gtgcacttat gggagttaca gaaccttcag tgtttggtgt gtttatgaaa
     2461 tatagaagac catttgtagc agtaatgata ggtggaggta taggtggttt aatagcaggt
     2521 ttagctcaag ttaaaacata tggaatggtt tggggattag ctgcattacc aacatatctt
     2581 gttggaggag aggtatctaa ctttacattt atgataataa gtgtagtagt atcatttgta
     2641 gcagcaacag cagcttccta tttactagga ataccttcag atgaaaaaga agaagttaag
     2701 ggtgataatg aaaaagaatt agaactaata atcaacgaga acaattcaag aactattagt
     2761 attggaaatg ttgcaaaagg tcaggtaatt gcacttgggg aagttaatga caaagcattt
     2821 tcatctggag cattaggtaa aggggttgga gttataccta gtgaatcaaa atcaactgtt
     2881 atatctcctg tagatggaac tataactata gtatttccaa ctaaacatgc atatggtata
     2941 gtgactgatg aaggattaga aatattaatt catattggga tagatactgt aaacttagat
     3001 ggaaaattct ttgaaagtct tgtaacacaa aatcaaaggg ttaaaaaagg agatgcttta
     3061 gcagtatttg aatctgataa aattatagag gaaggctttg attctactat aataactgta
     3121 gttacaaata ctagcgatta tttagatgta atctcatcaa atgatgaatc agaagaatta
     3181 ttaactgtaa tagtataaat tttaggaggg aaaattcatg aatattaaag atacattttt
     3241 ctggggagga agtattgcag cccatcaatg tgaaggttca tgggatagtg ataacaaagg
     3301 acctgcaatt atggattttg taactaaggg ctcttatgaa acgccaagag taattactga
     3361 taaaatagaa gaaaaattgg attatccatc tcataatgga atagattttt ataatagata
     3421 taaagaagat atagctttat ttaaagaaat gggatttagt gcattaagaa tatctataga
     3481 ttggtcaaga atttttccaa atggagatga tgaaaatcct aatgaattag ggattaaata
     3541 ctatgaaggt ctgatagata cattaataga aaataatata gaacctattg taacactata
     3601 tcattttgaa ttacctatga atttagtaca taaatatggt tcatggaata acagaaaact
     3661 tatagatttg tatttaaagt acagtgagac tgtaataaga agatttgatg ataaggtaaa
     3721 gtactgggtt acatttaatg agatgaacca tattgaccct caaacagagc actcggatat
     3781 ttttacttat atgatagcag gtattaagta tagtgaaata gaaaataagg ttcaaacatt
     3841 ggcaaatgta gggtataata tgactttggc gggtgtaaag gttgcaaagt tggctagaga
     3901 aatcaatccc aacaataaag ttggatgtgt atttggacta aatccaattt atccatacaa
     3961 ttgtaatcct agtaatgtgt taaatgcatt tttagataat gaccgtgatt attatcaaat
     4021 tgatgcaatg tgcaatggga agtttccaca atataaatta aaagaatatg aaaaaaataa
     4081 tataactatt gaagtgactg atgaagacaa aaaagcattt aaagaaggta agttagattt
     4141 tataggactt aattattatt tttcaagtgt atctactcca aaagaaaatt tatcaggtga
     4201 taaatcttta tttggtggga ttcaaaatcc atatttggaa caaagtaagt gggggtgggc
     4261 aatagaccct ataggtttaa gatttacctt gaactatatt tacagaaagt atcaattacc
     4321 tatcttaatt acagaaaatg gtttaggggc atatgataaa atcgaagaag atggaactat
     4381 aaatgatgat tatagaattg aatatttaag taagcatcta tctcaaatga agtctgcaat
     4441 tgaagaggat aatgttgaat gttttggata tttgatgtgg ggaccaattg atttagtcag
     4501 tgcaactaca ggtcagatga gtaagagata tggatttatc tatgttgact tagatgatga
     4561 aggcaaagga agttttaaga gatataagaa aaaatcattt gattggtaca aagaagtaat
     4621 aaaatctaat ggagaagttt tagatttttc tacggatata gtagtagggt aggaataaga
     4681 taaatagaag ttaggaacta atgttaatta gtgtctagta aagcatttaa aatagagtat
     4741 agggtcttta aagtaaagtg tgtattcttc ttaaaagtaa tggcaactaa aattactata
     4801 aaaaggagaa tacacatttt ttatgtttat acttaagtaa atatttagac atattcatag
     4861 tataatttac tcattatata aaaatagtat gatttactca ctatataact ttataaattt
     4921 tctggaaaaa tgtcagaata tattgacaaa gaaaagattt aaa
//
LOCUS       DAELLV010000245         4959 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.245, whole genome shotgun sequence.
ACCESSION   DAELLV010000245 DAELLV010000000
VERSION     DAELLV010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4959
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.245"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(131..568)
                     /locus_tag="KPD58_003213"
     CDS             complement(131..568)
                     /locus_tag="KPD58_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HBG0266944.1"
                     /translation="MKIHVEQGINCDDIEITIKCSALDNNIEKIISLLSTPLLDIKGK
                     KSGEIYKLNVCDILYFETVGNKTFAYYEDDVFEIDLKIYKLSKLLANTSFSRISKSMI
                     LNMDYIKSIKVISHNRMQATLKNNEKVIINRRYIKNIKEKLNI"
     gene            complement(825..974)
                     /locus_tag="KPD58_003214"
     CDS             complement(825..974)
                     /locus_tag="KPD58_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HBG0266945.1"
                     /translation="MDDSLQLLITNVGFPIALSMYLLVRIEGKLNTLSNSIDTLSKNI
                     LIFKK"
     gene            complement(987..1217)
                     /locus_tag="KPD58_003215"
     CDS             complement(987..1217)
                     /locus_tag="KPD58_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HBG0266946.1"
                     /translation="MEIKKKLVMSFKNVKDKQVSFSIENPKDDLTEENIKSVMDLIVS
                     KNIFSVGGFDLASVVEAKVVETNTTPYDLVIE"
     gene            complement(2035..2256)
                     /locus_tag="KPD58_003216"
     CDS             complement(2035..2256)
                     /locus_tag="KPD58_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HBG0266947.1"
                     /translation="MEVVSTKNLSSIKLKLDAGFDDKGKAVVKSKSFANVKAEALNED
                     VYAVAEAIASLQENPIVDILKLDSTSLSK"
     gene            2836..3093
                     /locus_tag="KPD58_003217"
     CDS             2836..3093
                     /locus_tag="KPD58_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG0266948.1"
                     /translation="MKIKVEDIPVQFHAMVEIIGIDKFIEVAKLYGGTNTYIPTYKGL
                     FRYARNRDIVKQFNGVNHNELAIRYNMCVSNIKRILNENNV"
     gene            3562..4800
                     /locus_tag="KPD58_003218"
     CDS             3562..4800
                     /locus_tag="KPD58_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HBG0266949.1"
                     /translation="MNKGFYKLNSVVLNFDIKNDVNKIRFNHYIMRKENIQRLQGTLP
                     NGQFYMTVRRAATDLQLSISTISRLVKEFLDLGIIRLVSKGVKGNRSVYSYVSIEFNG
                     SLFDDSKYMELDCVCNDEFIDNSGIEFDCDSKDNSFNGDINVETVHCINEYLSDDDVE
                     HNYNNFHNKDTIKDKAISNISTIKKYIDESFDKNKSENSNLQQESINFNFNDTCNEID
                     KRTNNSIENNQNKRPMRLSDISVLSDKGMTNKKNYFNHNHGIQKGTKSVTKKKESLNK
                     FNKKNIQQSIINMLNKKSGKNFKIDSPISIKLINERLKEGYVLEDFYKVIEVKVAKWK
                     NTIMEMYIRPETLFSYKFESYVNENISMKSQSNVHPCKEGNSNDSSNYIHSDYTTYDG
                     SVRERKKYENVRYWNPICEI"
BASE COUNT         1900 a          490 c          689 g         1880 t
ORIGIN      
        1 ttgattaatt atttttacac taaatataat taattagtat ttaatattga atatattata
       61 ttattattaa ttattaaata ggtcaataaa tttgcaatta gtgatattaa taaattactt
      121 tagattttta ttaaatgttt agcttttctt taatattttt tatataacga cgatttataa
      181 taactttttc gttgtttttt aaagtagctt gcatgcgatt atgtgatatt acttttatac
      241 tttttatgta gtccatattt aaaatcatgg atttagatat tcgtgagaag gatgtattag
      301 ctaataattt tgataattta tatattttta aatcaatttc aaatacatca tcttcataat
      361 aagcaaatgt tttatttcca actgtctcaa agtataaaat atcacagaca ttgagtttat
      421 aaatctctcc tgatttttta ccttttatat caagtaacgg tgtactcaat aatgaaatta
      481 ttttttcaat gttattatct agagcagaac attttatggt tatttcaata tcatcacaat
      541 taataccttg ttcgacatgt attttcatta atcatcacct cattttatta ctgtcattag
      601 aatagcatta tatcttattg agtccaacta gatgcgttct aaatttcaaa ttaagcatag
      661 tgaaattcaa aaataaaaca gtgaaattca aatttgaggt ggtaaaattc aaaaaaatac
      721 tatggatatt tggataaatt atccaaatat cctagtattt cacatagaat atgtctaatt
      781 attttacaaa tagacctatt ttatgactta catatattgt caaattactt cttaaatatc
      841 aaaatatttt tggataatgt atcaatactg tttgataaag tatttaattt accctcaatt
      901 ctaaccaata agtacatgga tagagctata ggaaaaccaa cattggttat taacagttgt
      961 aaactatcat ccataaaatt agccccctat tctataacta agtcataagg agtggtattt
     1021 gtttccacaa ccttggcttc aacaacagaa gctaggtcaa atcctccaac cgaaaaaata
     1081 tttttagata ctatcaaatc catgacagat tttatatttt cttcagttaa atcatccttt
     1141 ggattttcta tagaaaaaga aacttgttta tcctttacat ttttaaagct catgactaat
     1201 tttttcttaa tttccatata attacctccg atataataat ttatatttta attgtttgct
     1261 tgttaatatt ggtattttaa ttgtcaatat taatgttttg cttgttagta ttaatatttc
     1321 aattgtcaat attaatgttt tgcttgttag tattaatatt tcaattgtca atattagtgt
     1381 cttgcttgtt agtattaata tttcaattgt caatattaat gttttgcttg ttagtatcag
     1441 tgtttcaatt gtcaatatta gtgttttgct tgttagtatt aatatttcaa ttgtcaatat
     1501 tagtgtcttg cttgttagta ttagtatttc aattgtcaat attagtgttt tgcttgttag
     1561 tattagtatt tcaattgtca atattagtgt tttgcttgtt agtattagta tttcaattgt
     1621 caatattaat gttttgcttg ttagtattag tatttcaatt gtcaatatta gtgttttgct
     1681 tgttagtatt aatatttcaa ttgtcaatat tagtgttttg cttgttagta tcagtgtttc
     1741 aattgtcaat attagtattt taattgttga ctctagttat tgctgatata gaaaatattt
     1801 aatttatttc aattaattag attaaagtag ggtagcatct aaaatagcta acatgtttaa
     1861 gttgaaatac gtttaatata ttaatttttc aaagcattaa tatattaaag ttataatcaa
     1921 aattgaagtt ataatctgcc aaatctatag cgtattaagt gcgttatata acttaatctt
     1981 ttatataaag agttaatttt tttaatttgt aaagcagagt acgtttaaaa ggtactattt
     2041 agataatgat gtactatcta attttaaaat atctacaata gggttctctt gaagacttgc
     2101 tattgcttct gcaacagcat aaacatcttc atttaatgcc tctgctttta catttgcaaa
     2161 tgatttactt ttaacaactg ccttaccctt atcatcaaaa ccagcatcca attttagttt
     2221 tatactagat aaattttttg ttgaaacaac ttccataaaa ttacctccta aaaatttaat
     2281 ttcaaagatt tgtcgacttt ctttgttagc tataatacta tcatctaaga taatatcgga
     2341 gttaaacagt aatttctaaa gattttttgt gtaaattttt ataggaaaaa ttttactttt
     2401 ttgctgatga aaaagtttac aagttatttg tgaagatatg ttcttagtgt tttgaaatag
     2461 tatttgtata tattcatgct tagttgagat agttttgtat ggattttcat atgtgtagag
     2521 ttaaatgtta ggtactaaag tttatgttaa attaaatgtt aaagcttatg ttaaaaattt
     2581 tattaaattt aatctgtaaa taaaaaagtt aaaagtccaa tattaacatt ttacttttaa
     2641 accattaaaa tctttattaa agctaacaaa gttataagtt atgagaaatt ttattaaagt
     2701 taatacaaaa attaatttta aagctaagaa attttttatt agattcaata aaactaataa
     2761 atatagttta gtcaataatg tattaaataa aaactttgtt aagtctataa aaatatctta
     2821 aaggaggtgt aacttatgaa gataaaagtg gaggatatac cagttcaatt tcatgcaatg
     2881 gtagaaataa ttggaattga taagtttata gaggttgcca agttgtatgg aggaacaaat
     2941 acttatatac ctacgtataa ggggcttttt agatatgcta gaaataggga tatagtaaaa
     3001 caatttaatg gagtcaacca caatgaattg gctattagat ataatatgtg tgtaagcaat
     3061 attaagcgaa tactcaatga aaataatgtt taaatataat aaaataatgt ttaaacataa
     3121 tgaaaatagc cttgttaaac gtaagagtaa agacaaagaa gatgttttat aaagtttttt
     3181 tagacaaatt aactaaattt actacttatt aatttaaata cattgtatat tctataaaaa
     3241 aatttgttaa atactggaga tatattgaga atacaaagaa aatggtcgaa aatatttgtt
     3301 ggctaaaaaa tagataaaat gatacaattg cttgtataaa ggggaaaaat aaacaaataa
     3361 actatataaa taaagaaata gcaattaata tgacataata tatttataaa attaagaata
     3421 taaatagata ttttaaggta ttagttgtta aattaggaaa tattaaaaaa aatatatatt
     3481 tatatttaaa ctattttgtt atttatataa aaatacttat ctagttacta gcaattatta
     3541 gtagaaatgg gggaaataga gatgaataag ggattttaca aattaaattc agtggtattg
     3601 aactttgata ttaagaatga tgttaacaag ataagattta atcattatat aatgagaaaa
     3661 gaaaacatac aaagacttca aggaactctg ccaaacggac aattttatat gacagtgaga
     3721 agagctgcta cagatttaca actttctatt tctactataa gtaggcttgt aaaagagttt
     3781 ttggatttag ggataataag attagtgtct aagggtgtaa aaggtaatcg ttctgtatat
     3841 agttacgtaa gtatagagtt taatggaagt ttatttgatg atagtaaata tatggaactt
     3901 gactgtgtat gtaatgatga atttattgat aatagtggca tagagtttga ttgtgatagc
     3961 aaagataatt cttttaatgg agatataaat gtagaaactg tgcattgcat taatgagtat
     4021 ttgtctgatg atgatgtaga gcataattat aacaatttcc ataataaaga tactataaag
     4081 gataaagcca tttctaatat tagcactatc aaaaaatata ttgatgaatc ttttgataaa
     4141 aataaatcag aaaattctaa cttacaacag gagtcaataa atttcaactt taatgataca
     4201 tgcaatgaaa tagataaaag aacgaataat agtatagaaa ataatcaaaa taaaaggcct
     4261 atgagattga gtgacatcag tgttttaagt gataaaggta tgacaaataa aaagaattac
     4321 tttaatcata atcatggtat acaaaaaggg actaaatctg taacaaagaa aaaagaatcc
     4381 ttaaataaat ttaataaaaa aaatatacag caaagcatta taaatatgct aaataaaaaa
     4441 tcaggtaaga attttaaaat cgattctcca ataagcataa aacttatcaa tgaaaggcta
     4501 aaggaaggct atgtcttaga agatttctac aaagttattg aggtaaaagt ggctaagtgg
     4561 aaaaatacca taatggagat gtatataaga ccagaaacct tgtttagcta taagtttgaa
     4621 tcctatgtaa atgaaaacat atctatgaaa agtcaaagta atgttcaccc atgtaaagaa
     4681 ggcaatagta atgatagcag taattatata cattctgatt atacaacata tgatggcagt
     4741 gtgcgtgaga gaaaaaaata tgagaatgta agatactgga atcctatatg tgagatttaa
     4801 ttagtttaag aatgtattta tgataaataa gatattaata gagaatagtg agctatagca
     4861 aatgatatat caatagttgg tagtaagtta tattaagtaa gatattacta tcaggtagtg
     4921 aattatattg aacgatatat caataattgg tagtaaatt
//
LOCUS       DAELLV010000246         4948 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.246, whole genome shotgun sequence.
ACCESSION   DAELLV010000246 DAELLV010000000
VERSION     DAELLV010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4948
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.246"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     misc_binding    275..506
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            614..2095
                     /locus_tag="KPD58_003219"
     CDS             614..2095
                     /locus_tag="KPD58_003219"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HBG0266950.1"
                     /translation="MYYSSTRGTEEKVTASQAIIKGISNDGGLYVPSEFPNVKNELIN
                     LVNLTYSQIAFFVLSKFLCDFTEDEIKNCIENAYDEKFDCSSIAPLNKVNDTYFLELY
                     HGPTLAFKDMALTIMPHLLKTSIKKDNLEKDVVILTATSGDTGKAALEGFKDIDKIKI
                     IVFFPEDGVSPVQKLQMKTQTGKNTYVVGIKGNFDDAQSGVKKIFSDKEFNQKLLDNN
                     YILSSANSINIGRLLPQVAYYFYSYMKLVSNSDINLNDKINFVVPTGNFGNILAGYYA
                     KQMGLPINKLICASNDNNVLYDFFKTGVYDKNRTLKLTTSPSMDILISSNLERLLFEI
                     SNRNTDIVNKLLSDLSDKGVYKISDDMEKNLASFYGEYSNEEEVKSTINNVFKNYNYL
                     IDTHTAVAYNCYEKYKKETSDNTKTVIVSTASPFKFSEDVLKSIDCDFKDLDDFAIID
                     RLSSISKIDIPKPIKNLKNAEILHKDIYEKDELKLAIKKFLKV"
     gene            2105..2998
                     /locus_tag="KPD58_003220"
     CDS             2105..2998
                     /locus_tag="KPD58_003220"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HBG0266951.1"
                     /translation="MLEIIVPATSANIGPGFDCLGIALNIYNKFYVEEIESGLEIEGC
                     EDAYKNENNLVYTSMKYFFDRVKPEKIPAGIKIKIQSEVPICRGLGSSASCIVAGVIA
                     ANALSGANLDKNQLLNIASEIEGHPDNVAPAILGNMIVSVTDNENIHYDIIKIPEELK
                     FCAMIPNFKLSTEKARGVLPKEIPYSDGVFNVSRVALLISALLNKNFDLLKVACQDKL
                     HQDYRGTLIENYNDIVEKSEQLNSIGVFLSGAGPTIMSLIKENDDSFVDNMKNYLQKL
                     KSDWEIKELCCDSNGAVLNII"
     gene            3122..4183
                     /locus_tag="KPD58_003221"
     CDS             3122..4183
                     /locus_tag="KPD58_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HBG0266952.1"
                     /translation="MIIKELIIRDLVAKVPIIQGGMGVGISRSSLAGAVAKLGGVGVI
                     SGVQIGYDEEDFETNTINANLRAIKKHISKAKEISNGGIIGINFMVAMKEYETYVKEA
                     VKAGVDLIISGAGLPNKLPSLVKGSNVKIAPIVSTAKAANVILKMWDRKEKTTADLIV
                     VEGPKAGGHLGYSNEELDNIDSIDYDKEFVEILKVANTYGEKFGRNIPVVAAGGITSS
                     SDVKKYIDMGASGVQVGTRFVATYECDAHENFKMAYINASEEDVHIVKSPVGLPGRAI
                     RNKFIEEVKINRPEIKKCYNCLIPCNPKETPYCISQALINAVKGDVENSLLFCGNDAY
                     KIDKLSTVEDVINELISEL"
     gene            complement(4338..4850)
                     /locus_tag="KPD58_003222"
     CDS             complement(4338..4850)
                     /locus_tag="KPD58_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBG0266953.1"
                     /translation="MSDFKEILAEEIKNNPFGLIGKDWTLITAENEGKVNTMTASWGG
                     LGVMWGKDVAFVVIRPQRYTKEFIDNTDKFSLTFFDEDFRKELSYCGKVSGREEDKIS
                     QIGFNIEHLNDTPYFKESKMAIICKKMYSQKLEPQCFIAEGIDGRWYPQKDYHTLYIA
                     EITNVLVKED"
BASE COUNT         1841 a          608 c          767 g         1732 t
ORIGIN      
        1 agttttgtaa actttttttg taatatagtg tagtataaaa ttgtaatatt acagatattt
       61 aatagttatc ttttaattac tttcattaaa aacagcttca tcactatgaa aatctaggtt
      121 tttaaacttt taaaaaagtt aaaaaaatat ttttctaata ctcaatttga ttacaatttt
      181 tttacaattt aaaaaatatt ttttccaaat ttttatatta atacttgact ttgtctgttt
      241 tataaactag aataattaat aatattaaga atattgaaag gcattgatta ggaagagtaa
      301 atctgaatcg attaccagag aaggaaacta ctagctgaaa tgtttcccta attagaagga
      361 ttgaaaacta cctaggagct tctatccgaa gttatcttaa tgattttagt aggaatagac
      421 gtttttatta cttgcgttaa agtatcaagt ggatttttta aaatcaattt gggtggaacc
      481 gcgggaaaca gattctcgtc ccttttttag ggatgagggt ctttttttat tacctaaatt
      541 cagtttttaa aatattctgt atctaattta attagctaat taaaaattta aaatatatgg
      601 aggattataa aaaatgtatt attcaagtac gagaggcacc gaagaaaaag taactgcatc
      661 acaagcaata ataaaaggaa tatcaaatga tggtggtctt tatgttccaa gtgaatttcc
      721 caatgtaaaa aatgaactta taaatttagt aaatttaacg tattctcaga tagcattttt
      781 tgtacttagt aaatttttat gtgattttac tgaagatgaa attaaaaatt gtatagaaaa
      841 tgcatatgat gaaaagtttg attgtagtag tatagctcca ttaaataaag taaatgatac
      901 ttatttctta gaactatatc acggtccaac tttagcattt aaagatatgg cacttactat
      961 aatgcctcat cttcttaaaa catctataaa gaaagataat ttagagaaag atgttgtgat
     1021 tcttactgct acatctggag atacaggtaa agctgcactt gagggattta aagatataga
     1081 taaaataaag ataattgtat tcttcccaga agatggtgta agcccagtac aaaaacttca
     1141 aatgaaaact caaactggaa aaaatacata tgtagttggt ataaaaggaa attttgatga
     1201 tgcacaatca ggtgtaaaga agattttttc tgataaagaa tttaatcaaa aactattgga
     1261 taataattat atattatctt ctgcaaattc aattaatata ggaagacttc ttccacaagt
     1321 tgcatactat ttttattcat atatgaaatt agttagcaat agtgatataa atttaaatga
     1381 taaaataaat tttgttgtcc caactggtaa ttttggtaat attctagctg gatattatgc
     1441 caaacaaatg ggtcttccaa taaacaaatt aatatgtgca tcaaatgata ataatgttct
     1501 ttatgatttc tttaaaactg gtgtatatga taaaaataga acattgaaat taactacttc
     1561 tccttctatg gatatattaa tttcaagtaa tttagaacga ttactgtttg aaatatcaaa
     1621 tagaaatact gatattgtaa ataagctttt atcagacctt agtgataaag gagtctacaa
     1681 aataagtgat gatatggaaa aaaatttagc ctcattctat ggagaatatt caaatgaaga
     1741 agaagttaaa agcacgataa acaatgtgtt taaaaattat aattatttga tagatactca
     1801 tactgctgtt gcatacaatt gttatgaaaa atataaaaaa gaaacttctg ataacacaaa
     1861 aactgttatt gtaagtactg caagtccatt caagttttct gaagatgttc taaagtctat
     1921 agattgtgat tttaaagacc ttgatgactt tgctataatt gatagattat caagtatttc
     1981 taaaatagat attccaaaac ctattaaaaa tcttaaaaat gcagaaatac tacataaaga
     2041 tatttatgaa aaagatgaat taaaactagc tataaaaaaa ttcttaaagg tataggtgat
     2101 tattatgtta gagattatag ttcctgcaac aagtgctaat ataggtccag gatttgattg
     2161 tcttggaatt gcgttaaata tatacaataa attttatgtt gaagaaattg aaagtggtct
     2221 tgaaatagaa ggttgtgaag atgcttacaa aaacgaaaac aacttagttt atacatctat
     2281 gaagtacttt tttgatagag ttaaaccaga aaaaatacct gctggtatca aaataaaaat
     2341 ccaaagtgaa gtacctatat gtagaggtct tggcagtagt gcctcttgta tagtagctgg
     2401 agttatagct gcaaatgctc tatcaggtgc taatttagat aaaaatcaat tactaaatat
     2461 agcttcagaa atagaaggtc acccagataa tgttgctcct gctattttag gaaatatgat
     2521 tgtatctgtt actgataatg agaatataca ttatgatatt ataaaaattc ctgaagaact
     2581 taaattttgt gcaatgatac ctaattttaa gttatcaaca gaaaaagcta gaggtgtttt
     2641 accaaaggaa ataccatatt ctgatggggt attcaatgtt agtagagttg ctttactaat
     2701 atctgcctta ctaaacaaaa actttgacct acttaaagta gcttgtcaag acaaattgca
     2761 tcaagattat agaggaactc taattgagaa ctacaatgat attgttgaaa aatctgaaca
     2821 attaaatagt attggagtat ttttaagtgg agctggacct actataatgt ctttaataaa
     2881 agaaaatgat gatagttttg tggataatat gaaaaattac ctacaaaaac taaaatctga
     2941 ttgggaaata aaagagttat gttgcgattc taatggagct gttttaaata taatataatt
     3001 atcgtcttct atacaaaata taactactct tttattataa tttgtagtat aataattagg
     3061 actaggtaat aatattaagt attataatct agttctttaa acatgtttac attgaggagg
     3121 gattatcata aaagagttaa taattagaga tttagttgca aaagttccta taattcaagg
     3181 tggaatgggc gttggtatat ctaggtcatc actagctggt gcagttgcta agcttggtgg
     3241 tgttggtgta atttctggag ttcaaattgg atatgatgag gaagattttg aaactaatac
     3301 tataaatgca aatttaagag ctataaaaaa acatatttct aaggccaaag aaatctcaaa
     3361 tggtggtatt attggtatta attttatggt agctatgaaa gaatatgaaa catacgtaaa
     3421 ggaagctgtt aaagctggtg tagaccttat aatatctgga gctggattac ctaataagct
     3481 accatcatta gtaaagggaa gtaatgtaaa aatagcaccc atagtttcaa ctgcaaaagc
     3541 tgctaatgta attttaaaaa tgtgggatag aaaagagaaa actactgctg acttaatagt
     3601 tgttgaaggt ccaaaagctg gtggtcacct tggatactct aacgaggaac ttgataacat
     3661 agattctata gactatgata aggaatttgt tgaaatttta aaggttgcaa atacttatgg
     3721 agaaaaattt ggtagaaaca taccagtagt tgctgctggt ggtataactt ctagtagtga
     3781 tgttaaaaaa tatatagata tgggtgcaag tggtgtacaa gttggtacaa gatttgttgc
     3841 tacttacgaa tgtgatgccc atgagaactt taaaatggca tacataaatg ctagtgaaga
     3901 agatgttcat atagttaaaa gtcctgtagg tcttcctgga cgcgccataa gaaacaaatt
     3961 tatagaagaa gttaaaataa atcgtccaga aataaagaag tgctataatt gtttgattcc
     4021 ttgtaatcca aaagaaactc cttactgtat ttctcaagct ctaatcaatg cagttaaagg
     4081 agatgttgaa aattcacttt tattttgtgg aaatgatgcc tataaaattg ataaattaag
     4141 tactgttgaa gatgttatca atgaattgat ttctgaatta taatttaaag aaaactaaat
     4201 taagatttta tcaagctaag gagtttaaat acgttattta ttaatcttta taaacatatt
     4261 tatgtcctta gcttttttgt gtcaatttcc ttttattgaa attttaattt aattagagat
     4321 agtattaacc cttataacta atcttctttt acaagtacat tggttatttc agctatatat
     4381 aaagtatgat aatctttttg tggataccat cttccatcta taccttcagc aataaaacat
     4441 tgaggttcaa gtttctgact atacattttt ttgcatataa tagccatttt agattcttta
     4501 aaataaggtg tatcatttaa gtgttctata ttaaatccaa tctgtgatat cttatcctct
     4561 tctctaccag atacctttcc acaatatgaa agctctttac gaaaatcttc atcaaaaaat
     4621 gtaagagaaa atttatctgt attatctata aattccttag tataacgctg tggtcttatt
     4681 actacaaaag caacatcttt tccccacata actccaagcc ctccccagct agcagtcatt
     4741 gtattcactt taccttcatt ttctgctgtt ataagtgtcc aatctttacc tatcaatcca
     4801 aatggattat ttttaatctc ttctgccaaa atttctttaa aatcactcat atctctaata
     4861 acctctcatt ctcttatatt tatattcctt ttattttcgt gataaattat aaatgaagtc
     4921 tagttttgaa aataaaatac ttttttaa
//
LOCUS       DAELLV010000247         4911 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.247, whole genome shotgun sequence.
ACCESSION   DAELLV010000247 DAELLV010000000
VERSION     DAELLV010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4911
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.247"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            646..4788
                     /locus_tag="KPD58_003223"
     CDS             646..4788
                     /locus_tag="KPD58_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HBG0266954.1"
                     /translation="MATVSGQIVFDRNRSATIDSGDSGIANIPVVLQDIATGVRLVVL
                     TDASGNYVFTNVPNGNYRIVEAFGTTGGVPTPGNFATAIVGPVPIAEMPPISSVTSPP
                     IGSTNLDGTTPNTLLITVAGSDITNQNILNGPVIYTPIENILDSCVSVSNINLITDAD
                     NGTFGFFPPGTPSNTGPEIEPYPNVTPDFTYVVPDPTKFTPLDGEYTVQNIMTNAMSN
                     VIGAWWRIADHTTGNETGRMMVVNGFNPGAVFFKSTVQVTPNTNYLFSTWILNLFKVT
                     GYPPPQLGVRIFDQNNNILYQATLGAQIPVNTNAPEWKEVGTVINSQNNNQLTVEFFS
                     EGESAIGNDYAIDDVSLREIAVPTFTPIKTSNTETANVGDIITYTVTLDNTCDSPLTN
                     VFFRDLIPNGLEFIPGTVTVDGVSVPGVDPNTGFPLPDIGGGTGVEVTFDVVVESIPN
                     PNPTNNIATIDYSYTPVEGGIPNDFSVDSNPVPVEVISADIEVTKLSEPTIVNPGEEL
                     IYTIKVVNNGPFPSENVVLTDDVPASIVNPEYSLDGGVTFQPWTGSLNIGTLEVGETR
                     VIIIRGIVNPSTVGIITNTAVVSSTTADPNLNNNTSTIETEVNLLADILVMKTAEPNS
                     AVPGTLLRYTIQVENLGPANAENVILNDDIPASIINPEYSLDGGASFQPWNGSLNIGT
                     LNSGISLTVLIQGTVSLNSSEYIVNTATVSSTTPDPDLSNNISTIITPVNPQAGISII
                     KVADEDVAVPGEEFVYTIEIFNEGPSNATNVVLTDDIPDVILNPEYSLDGGATFQPWT
                     GSLNIGTVAPGQLIRIIIKGLVSSTATGDITNTAEVSADVPEPVTDSSTVTTPIVPSA
                     DIEVIKTSNMDTAVPGETFSYTITVTNLGPSAAQSIVLTDDIPDVILNPEYSLDGGVT
                     FQPWNGNLSIGTLDAGEIRSIIIRGTVSQTAVGTIINTATISSPTPDPNPDNNTSTDE
                     TDISPLADISVIKGNEPVAIPGGRFIYGIEIANAGPSFTENVTLTDNIPASILNPEYS
                     IDNGVTFQPWNGSLNIGTLDAGEIRNIIIRGTVSQTAIGTIINTATVSSTTPDPNLNN
                     NTSTSEAEVSSSADISVVKRSNQIVVVPGDVLDYTIEVRNAGPSTTQNVTLTDNIPAS
                     ILSPEYSIDNGVTFQPWNGSLSIGTLDAGEIRNIIIRGIVSQTAIGTIINTATVSSTT
                     PDPNPDNNTSTSEIDISSSADISVIKLANKTEACVGEQIDFVIVVSNAGPENAQNVTL
                     IDNVSDKLKKAVFSLDRGVSFQPWTGSLNIGTLPAGTLRVILLRGIIKSTCLDRLTNI
                     AEITSTTPDSNLNNNISRVQVEIKQCCCNDCCCNNCSNCCCNDCGCKDCCCNCCKNDF
                     WC"
BASE COUNT         1899 a          724 c          827 g         1461 t
ORIGIN      
        1 ataataaaaa taaagtcaac taaaattatt gattttagat taaagtaccg gattaagcat
       61 ttaagtagat tttaagcttg atttgattag aaaggtacaa actatcaccg aaaaaaatta
      121 aacccattta aaaagcaata taaaagcttg ttatttttga gaaaaataga ccctctaaac
      181 tcaattagat taaaaataaa atacagaaaa ttatatctct aaatatctta aataattaag
      241 tttaaatttt acgatatttt gtttgatgat ttataatata aaagcatact cttaatcttc
      301 aaaatttaaa aagagtaaaa aataacaaaa aaatcactga catttcactg acaaattgta
      361 attttgttat tataaataca ttgaaaatac ttgattattg aaaaatggca taaaataata
      421 cattttatgc catttgctat tatttgatag tttttaactt atgacataag atatttttta
      481 acatatacaa taaaatataa aaaaattgat attgcaaata aaaaaaaata catattagat
      541 gttatagaaa aaaagcgcac ttataggctt aaaatgtcac ataaaagagt tttaaagaat
      601 aatatattat gaacacaaat tttgtaatca caaaggagtg ataatatggc aacagtatca
      661 ggacaaatag tttttgatag aaatagaagt gctaccattg attctggaga ctcaggaata
      721 gccaatatac cagttgtatt acaagatata gcaactggag ttagacttgt ggttctcaca
      781 gatgcatcag gaaattatgt ttttactaat gttccaaatg gtaactatag aattgtagaa
      841 gcttttggaa ctacaggagg agtaccaaca ccaggaaatt ttgcaacagc gatagtaggt
      901 ccagttccaa ttgcagaaat gccacctatt agttctgtga caagtcctcc tattggttca
      961 acaaacttag atggtacaac acctaacaca ctattaataa cagtagcagg ttcagatata
     1021 acaaatcaaa atatattaaa tgggcctgtg atttatacac ctatagagaa tatattggat
     1081 tcttgcgtat ctgtatctaa tataaacctt attacagatg cagataacgg aacattcggt
     1141 ttttttccac caggtacacc aagtaataca ggacctgaaa tagaacctta cccaaatgtg
     1201 acgccagatt ttacgtatgt tgtaccagac ccaactaagt ttacaccttt agatggagaa
     1261 tatacagtac aaaatattat gacaaatgca atgagtaatg ttataggagc ttggtggcgt
     1321 attgctgacc atactacagg aaatgaaaca ggaagaatga tggtggtaaa tggttttaat
     1381 cctggagctg tattttttaa atcaacagta caagttacac ctaacactaa ttatttattt
     1441 agcacttgga ttttaaacct ttttaaggta acaggatatc caccaccaca gctaggtgta
     1501 agaatatttg accaaaataa taatatacta tatcaagcaa cattaggagc tcaaattcca
     1561 gttaatacaa acgcaccaga atggaaagaa gtaggtactg tgattaattc tcaaaataat
     1621 aatcaactta cagtagaatt ttttagtgaa ggagaaagcg ccattggaaa tgactatgct
     1681 atagatgatg tttctttaag agaaatagct gttccaactt ttactccaat aaaaacaagt
     1741 aacacagaaa ctgctaatgt aggtgatata atcacatata cagttacatt agataatact
     1801 tgtgatagcc ctttaactaa tgtatttttt agggatttaa ttccaaatgg tttagagttt
     1861 atacctggaa ctgtaacggt tgatggagta tctgtaccag gagtagaccc caatacaggt
     1921 ttcccacttc cagatattgg aggaggaaca ggggttgaag taacttttga tgttgtggtt
     1981 gaaagtattc ctaacccaaa tcctactaat aatatagcta ctatagatta ctcctataca
     2041 cctgtagaag gcggtatacc aaatgatttt tcagtagatt ctaatccagt tcctgttgaa
     2101 gtaatctcag cagatattga agttacaaaa ttatctgaac ctactatagt gaatccagga
     2161 gaggaattaa tatacactat aaaagtagta aataatggac cattcccatc tgaaaatgtg
     2221 gttttaacag atgatgtacc agcttctatt gtaaatccag agtattcatt agatggagga
     2281 gtaacttttc aaccatggac tggaagttta aatataggga cattagaagt aggagagaca
     2341 agagttatta taattagggg tattgtaaat ccatcaacag taggaattat aacaaataca
     2401 gctgtggtat cttcaactac agcagaccca aaccttaata ataatacatc tacaatagag
     2461 acagaagtga atttattagc agatatttta gtaatgaaaa cagcagaacc taattcagct
     2521 gttccaggta ctttattgag atatactatt caagtagaaa acttaggacc agcaaatgct
     2581 gaaaatgtaa tattaaatga tgatattcca gcttctatta taaatccaga atattcattg
     2641 gatggaggag caagttttca accatggaat ggaagtctta atataggaac tttaaactct
     2701 ggaatttcat taacagtttt aattcaagga acagttagtt taaattcttc agaatatata
     2761 gtaaatacag caacagtttc ttcaactaca cctgacccag atttaagcaa caatatatct
     2821 accattatta caccagttaa cccacaagca ggtatttcta ttataaaagt agctgacgag
     2881 gatgtagctg ttccaggaga agaatttgta tatacaattg aaatttttaa tgaaggacca
     2941 tctaatgcta cgaatgtagt tttaacagat gatataccag atgttatttt aaatccagaa
     3001 tattcattgg atggaggagc aactttccaa ccatggactg gaagtcttaa tataggtaca
     3061 gttgcaccag ggcaattaat aagaattatc ataaagggat tagtaagttc aactgctaca
     3121 ggagatatta ctaatacagc agaagtaagt gcagatgtac cagaaccagt tacggattca
     3181 tcaacagtga caactcctat agttccatca gcagatatag aagtaataaa aactagcaat
     3241 atggatacag cagttccagg agaaactttc agttatacta taacagttac aaatttagga
     3301 ccatctgctg cacaaagtat cgttttgaca gatgatatac cagatgttat tttaaatcca
     3361 gagtattcat tggatggagg agtaactttc caaccatgga atggaaatct tagtataggc
     3421 actttggatg ctggagaaat aagaagtatt ataataagag gaacagtgag ccagacagca
     3481 gtaggtacaa ttataaatac agcaacaata agttcaccta cacctgaccc aaatccagat
     3541 aataatactt caacagatga aacagatata agtccattag cagatatttc tgtgataaaa
     3601 ggcaacgaac cagttgctat accaggggga agatttattt atggaattga aattgcaaat
     3661 gcaggaccat cttttacaga gaacgtaact ttaacagata atataccagc ttctatttta
     3721 aatccagagt actcaataga taatggagtg actttccaac catggaatgg aagccttaat
     3781 ataggtactt tagatgctgg agagataaga aatatcataa taaggggaac agtaagtcaa
     3841 acagcaatag gcacaattat aaatacagca acagtaagtt caactacacc tgacccaaat
     3901 ctaaataaca atacttcaac atcagaagca gaggtaagtt catcagcaga tatttctgta
     3961 gtaaaacgaa gcaatcagat tgttgttgta ccaggagatg tactagacta tacaattgag
     4021 gtgagaaatg caggaccgtc tactactcag aacgtaactt taacagataa tataccagct
     4081 tctattttaa gtccagaata ttcaatagat aatggagtga cttttcaacc atggaatgga
     4141 agtcttagta taggcacttt agatgctgga gagataagaa atattataat aaggggaata
     4201 gtaagtcaaa cagcaatagg tacaattata aatacagcaa cagtaagttc aactacacct
     4261 gacccaaatc cagataataa tacttcaaca tcagaaatag atataagttc atcagcagat
     4321 atttctgtaa taaaattagc aaataaaaca gaagcatgtg taggagagca aatagatttt
     4381 gtaatagtag tatcaaatgc aggaccagaa aatgctcaaa atgttacttt aatagacaat
     4441 gtttcagata agcttaagaa agcagtattt tcacttgata gaggtgtaag tttccaacca
     4501 tggactggaa gtcttaatat aggtacacta ccagctggaa ctttaagagt tattctatta
     4561 agaggaatta taaaatcaac atgtcttgat agattaacaa atattgcaga aattacttca
     4621 acaacaccag attcaaacct taacaataat atatcaagag tgcaagtaga aataaaacaa
     4681 tgttgttgta atgattgctg ttgtaacaac tgtagtaatt gttgttgcaa tgattgtggt
     4741 tgtaaagatt gttgctgtaa ttgttgtaaa aatgactttt ggtgttgatt ttataaaata
     4801 attttgtata ccttaaatta aactgaaata aaaaataaat tcacatatga gtaaaatatt
     4861 ttataaatat taataaattt tctatctttg atatggacca cattcgtaat a
//
LOCUS       DAELLV010000248         4874 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.248, whole genome shotgun sequence.
ACCESSION   DAELLV010000248 DAELLV010000000
VERSION     DAELLV010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4874
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.248"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..429
                     /locus_tag="KPD58_003224"
     CDS             <1..429
                     /locus_tag="KPD58_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266955.1"
                     /translation="KQQLNELNKLEKDKNINIDEVSSIFGNIMGEVFVYKKDENERNL
                     RMIGFNIGKYIYLLDAYEDLDEDFKKGRYNPFIEYIDKNDELKEKVKKIIVTSLGFLA
                     RGIDNLNLNKNVGIIENIIYSGVYLRYINILESRGGKNVQ"
     gene            419..862
                     /locus_tag="KPD58_003225"
     CDS             419..862
                     /locus_tag="KPD58_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266956.1"
                     /translation="MYSNTYNNENFMRARSFIENSEFKKAYDFLKTLTDKCAEWYYLT
                     GFSAMNIGYYEEGEDFLKRAKFMEPENSEYSDALRSYTQYRNDYSNRADNYNRRRRND
                     LDGCCCCCCDDCCCCLGDDCCENCAKLWCLDSCCECFGGDLITCC"
     gene            873..1364
                     /locus_tag="KPD58_003226"
     CDS             873..1364
                     /locus_tag="KPD58_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266957.1"
                     /translation="MSIKVAYGGVLLALNVILLTLTNIIPVNTLFIMGLASLLVSIVI
                     MEWGFKSGIAFYIGSIVLGFIVMASKSQWIVYSLTFGIYGIVKYLIEKDRSIYIEYFM
                     KLVFANIMILILYFLLRTIVYIPINIFIIGSFEIAFIVYDYVYSSFIGYYNNRLRKML
                     FKK"
     gene            complement(1566..2294)
                     /locus_tag="KPD58_003227"
     CDS             complement(1566..2294)
                     /locus_tag="KPD58_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HBG0266958.1"
                     /translation="MKNIAAFFDIDGTLYRDSLMVEHFKKLIKYDIIDQKNWYKHARD
                     TFMDWDKRQGNYDDYLLEICDLYVDSLKGLDKTCINFTSDQVIKLKADRVYKYTRSRI
                     EWHLNQGHIVIFISGSPGFLVEKMAKKYNVTDFLGSDYVFENNIFTGTVIPMWDSISK
                     NNAINDFVVKYDLDLSNSYAYGDTNGDINMLKRVGNPIAINPTKELLSQISLDEEISK
                     NVEIIVERKDIIYSLSTDVNIVDI"
     gene            2419..3507
                     /locus_tag="KPD58_003228"
     CDS             2419..3507
                     /locus_tag="KPD58_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266959.1"
                     /translation="MNFNFAKATNSRLMGSLGLIINWIDDENNHFCQYFLLDAEGLGL
                     ADYVSLNNPTQEEAYMEEERLMGGFGSDRVELTKDESLFLVSYFGNKNLYYDKLLPGD
                     KCEYIDIIKNYKTDLTIEKLYNKICKRVDEEVEFINYMTMRFIAWDRESLKYFSGSDE
                     IANMHITNINGTLLKNVVSDKGQGRYISEALYEDNDGYYICKIAFCISKCYETGFRIN
                     SLLVTDKEPMYDFEVFDEISKSEFVSVYSVNSPEEFAGVFYRENPFLLKSNMNEGIFF
                     TRFNFNNDHVKENVYIINNDMKAIYYLMGNKFFIGTYNENDCNYINEISQSNYSDYIK
                     FEEKFFFEQNALYDFAESGSLDFDDFLE"
     gene            complement(3944..4792)
                     /locus_tag="KPD58_003229"
     CDS             complement(3944..4792)
                     /locus_tag="KPD58_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266960.1"
                     /translation="MSNKKKKDLDTSYMPYLDPANYADYITNEEIPQDELRNDPQISN
                     GYTKIPNNQNINNLYPNNVNHYPKSSNSPNNLPNTNNTSGNMNYNTSATNNMPNNMNN
                     NTGMPNNMSNNMNSNMGMPNNMPNNMNSNMGMPNNMPNNMNSNMGMPNNVPNNMNSNM
                     GMPNNMSNNMNSNMGMPNNMSGNMNNNMYGNTGMPNNMNSNMGYNMNNTMGIPNFSCN
                     QNMPPNVLMMIPGVICHNTMQGMPVVMPSTMPPNIYPTPYGSSNMSVQGIPQATNIEE
                     FDEEEM"
BASE COUNT         1777 a          504 c          757 g         1836 t
ORIGIN      
        1 aaacaacaac taaatgaatt aaataagtta gaaaaagata aaaatataaa tatagatgaa
       61 gtatctagta tatttggaaa tataatgggt gaagtatttg tctataaaaa agatgaaaat
      121 gagagaaact taagaatgat aggttttaat ataggtaagt atatatatct actagatgcc
      181 tatgaagatt tagatgaaga ttttaagaaa ggaagatata atccatttat agagtatatt
      241 gataaaaatg atgagttaaa agaaaaagta aaaaaaataa tagtaacatc attaggattt
      301 ttagctaggg gaattgataa tctaaatcta aataaaaatg ttggaataat agaaaatata
      361 atttattctg gtgtttattt aagatatatt aatatattag aaagtagagg tggaaagaat
      421 gtacagtaat acttataata atgaaaattt tatgagagca agatcattca tagaaaatag
      481 tgaatttaaa aaagcatatg attttttaaa aactttgact gacaaatgtg cagagtggta
      541 ttatttaact ggtttttctg caatgaacat aggatattat gaagaaggtg aagacttctt
      601 aaaaagagca aaatttatgg agcctgaaaa tagtgaatat agtgatgcac ttagaagtta
      661 tacacaatat agaaatgatt atagcaatag ggctgacaat tataatagaa gaagacgtaa
      721 tgatttagat ggatgttgtt gctgttgctg tgatgattgc tgttgttgtc taggtgacga
      781 ttgttgtgaa aattgtgcta aactttggtg tttagattca tgttgtgaat gttttggtgg
      841 agatttaata acttgttgtt aggatgataa atatgagtat aaaagttgct tatggaggag
      901 ttttattagc attaaatgtg atattgttga ctcttacaaa cattattcct gtaaatacat
      961 tgtttattat ggggttagca tcacttttag tatcaattgt aataatggaa tggggcttta
     1021 agagtggaat tgctttttat ataggttcta tagttttagg atttatagtg atggcaagta
     1081 agtctcaatg gattgtatat tcacttacct ttggaatata tggtattgta aaatatctta
     1141 tagaaaaaga taggtctata tatattgagt attttatgaa attagtattt gcaaatataa
     1201 tgatacttat attgtatttt ttattaagaa caatagttta tattccaata aacatattta
     1261 ttataggttc atttgagata gcctttatag tatatgacta tgtttacagt tcttttatag
     1321 gttattacaa taatagatta agaaagatgc tatttaaaaa ataatagttg atttttatat
     1381 ttaattttaa tgaaattgta gtttagataa gtgtaaatga tatatatgaa tatacattaa
     1441 agcataaagc taaatttaat atattcttag tgcaacatat aaaagctttg atttttagtc
     1501 aaagctttta tatgttagat ataaatatta gatataatat aagtttttaa aatagatagt
     1561 ataaattaaa tatccactat atttacatct gtagatagag aataaataat atcttttctt
     1621 tctactatta tctcaacatt tttagaaatt tcctcatcaa gtgatatttg agataaaagt
     1681 tcctttgttg gatttattgc tattggattt ccaactcttt ttagcatatt tatatcacca
     1741 ttagtatctc cataggcata cgagtttgat aaatctaaat catactttac aacaaaatcg
     1801 tttatagcat tattcttact tatagaatcc cacattggaa taactgtgcc tgtaaatata
     1861 ttgttttcaa agacataatc acttcctaga aaatcggtta cattatattt ttttgccatt
     1921 ttttcaacaa gaaatccagg gctaccagat ataaatataa ctatgtgtcc ttgatttaga
     1981 tgccactcaa tccttgaacg tgtgtattta taaactctat ctgcttttaa tttaattact
     2041 tggtcacttg taaagttgat acaggtttta tctaatcctt ttagtgaatc aacataaagg
     2101 tcacatattt ctaatagata atcatcataa ttaccttgtc ttttatccca atccataaaa
     2161 gtgtctctag catgtttata ccaatttttt tggtcaatta tatcgtattt aattaatttt
     2221 ttaaaatgtt caaccataag agagtctcta taaagagtac catctatatc aaaaaaagct
     2281 gctatatttt tcataagacc acctcaatta atcatttcct tatattatat tataaattgt
     2341 atctaagttg ttaaaatata tgtataataa tttatatatt aaaaaacaag tattttagag
     2401 tttaaaggat gtgactatat gaattttaat tttgcaaagg ctacaaattc tagacttatg
     2461 ggcagtttag gtcttataat aaattggata gatgatgaaa acaatcactt ttgtcaatat
     2521 tttttattag atgcagaggg attaggatta gcggattatg tgagtttaaa taacccaaca
     2581 caagaagaag cttatatgga agaagaaaga ttgatgggag gatttggttc agatagagtt
     2641 gaattaacga aggatgaatc tttattttta gtatcttatt ttggtaataa gaatctttat
     2701 tatgataagc tattgccagg agataaatgt gagtatattg atattataaa aaactataaa
     2761 acagatttaa ctatagaaaa attatacaat aaaatatgta aaagagttga cgaggaagtt
     2821 gaatttataa actatatgac catgagattt atagcatggg atagagaaag tttaaagtac
     2881 ttttctggaa gtgacgagat tgcaaatatg cacattacta atataaatgg aacattactt
     2941 aaaaacgtgg taagtgataa aggtcaaggc agatatataa gtgaagcttt atatgaagat
     3001 aatgatggat attatatatg taaaattgct ttttgcataa gtaagtgtta tgaaactggt
     3061 tttagaatta attctctatt agtgacagat aaagaaccta tgtatgattt tgaggtattt
     3121 gatgagattt ctaaaagtga atttgtaagt gtttacagtg taaattcacc tgaagaattt
     3181 gcaggagtat tttatagaga aaatccattt ctgttaaaaa gtaatatgaa tgaaggtata
     3241 ttctttacta gatttaattt taataatgac catgtaaaag aaaatgtata tataataaat
     3301 aatgatatga aagctatata ttacttaatg ggaaataagt tttttattgg tacttataac
     3361 gaaaatgatt gcaattatat caatgaaata agtcaatcta attatagcga ctatattaaa
     3421 tttgaagaga aatttttctt tgagcaaaat gcattatatg attttgcaga atcaggttca
     3481 ttagattttg atgatttttt ggaataataa atttagacat tatttaagcg agattaactg
     3541 aatttaatct cgcttaaatt tatttctata atattggggg aatattatga ggaatacatt
     3601 tattattgtg gtcaattatt aaaattttat acagtttatt ataaaaaatt tatatttttc
     3661 tttatgttgt ttgccaatat tagtctcaaa tacgagtacc atactattct gaatcaccaa
     3721 cacaagagag catttttaaa tgaaaaagtc agataaaatt aaattttatc tgactttttc
     3781 tatgtttatc ttattagatt atgttaatat ctgttgaccg aataagcgca tattcagtca
     3841 actttctata cctataataa tattggggga atattatgag gaatacattt attattatgg
     3901 tcaattatta atgttttata caacttactt aaaaaaatat atattacatt tcttcctcat
     3961 caaattcctc aatattagta gcttgtggta taccttgtac agacatatta ctagaaccat
     4021 aaggagtagg atatatatta ggaggcatag tactaggcat aactacaggc ataccttgca
     4081 ttgtattatg acatataact cctggtatca tcataagtac attaggaggc atattttgat
     4141 tacatgagaa gttaggaata cccatagtat tgttcatatt ataccccata ttactgttca
     4201 tgttattagg catacctgta ttaccataca tattgttatt catattgcca gacatattgt
     4261 taggcatgcc catattacta ttcatattat tagacatatt gttaggcata cccatattac
     4321 tattcatgtt attaggcaca ttgttaggca tgcccatgtt actattcata ttattaggca
     4381 tattgttagg catacccata ttactattca tgttattagg catattgtta ggcataccca
     4441 tattactatt catattgtta gacatattat taggcatacc cgtattatta ttcatgttat
     4501 taggcatatt atttgtagca gatgtattgt aattcatatt tccggatgta ttatttgtgt
     4561 taggcaagtt gtttggagaa ttactacttt taggataatg atttacatta ttaggatata
     4621 ggttatttat gttctggtta ttaggtattt ttgtataacc attagaaatt tgagggtcat
     4681 ttctcaattc atcttgggga atttcttcat tagtaatata atctgcatag ttcgctggat
     4741 ctaagtaagg catgtaacta gtatctaaat cctttttttt cttgttactc atattaaact
     4801 tgcacctcct agaaaactat aaaaaagttt acaaaaactt attctaataa tatatatatt
     4861 aaaaaagttt ataa
//
LOCUS       DAELLV010000249         4865 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.249, whole genome shotgun sequence.
ACCESSION   DAELLV010000249 DAELLV010000000
VERSION     DAELLV010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4865
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.249"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..634
                     /locus_tag="KPD58_003230"
     CDS             <1..634
                     /locus_tag="KPD58_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0266961.1"
                     /translation="EGLFYAFENDGFNVFKAYTKQEGLNIFNSKNIDFIILDCNLPDG
                     YGFDVCQIIREKSDIPIIMLTARDSEIDEVKGLEIGLDDYITKPFSLSVLKARVKVAI
                     RKKSNNKVIYSNGIKLDQKLLKVYKNKECLELSSVEYKLLSYLIENKGQILLKEQILH
                     HIWDSEENYVDDNIVSVNIRRLRVKVEDDPSNPKYIKTAYGMGYLWNEVE"
     gene            636..1544
                     /locus_tag="KPD58_003231"
     CDS             636..1544
                     /locus_tag="KPD58_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0266962.1"
                     /translation="MFILAFIICTVVIFIVSTKINRKRYDELNLMLNQILEGKEVTYP
                     DTKDTRASKISHQVKKIKDMIEIEVEQSKSEKEAIKGLISNMSHQLKTPLSNITIYCE
                     LLENINISTLQKKEFLQKMKNETFKIDWLLQSLFKMTKLEDGVIEFEVEELLIKDTLI
                     QSISTIFNKAEAKNIRINLEPFSDIKLVHNKKWTIEAIVNVLENAIKYSPSDSTITIS
                     VIKMELYTKITIKDEGIGIDSRELNDIFKRFYRSKNVENQNGTGIGLYLTRLILEKEN
                     GNIIVESKLGSGCCFSIFLQNCKSLN"
     gene            1621..2307
                     /locus_tag="KPD58_003232"
     CDS             1621..2307
                     /locus_tag="KPD58_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0266963.1"
                     /translation="MSILVTNNLVKHYGKGETKVKALNGVSIEIERDTFTAIVGTSGS
                     GKSTLLNIIGGLDNPTSGDVIIKGKNISKIGKKDLTVFRRRNIGFIFQNYSLMPVLNT
                     YDNIALPVTLDKGNHIDHEYIEMLMKTLGIWDKRLKFPSELSGGQQQRVAIARALANK
                     PALILADEPTGNLDSKTTMEVVCLLKESSAKFHQTILMVTHNENIAQVCDSIIHIEDG
                     VVVNTGGEVL"
     gene            2304..4859
                     /locus_tag="KPD58_003233"
     CDS             2304..4859
                     /locus_tag="KPD58_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG0266964.1"
                     /translation="MMSLVKFSIQYIKHYKKQSISIVLSIVLSVALLTGIGSLVHSAD
                     KSRIEKIREDSGDYHYFYKVDKEQLNKIKENKKSKEYTVDRLGITKPKNQIDEPFIIE
                     FLNADSSYLDMTGRKLLKGTLPSKDGEIVLDTYSLNNLNVNKEIGTEINLDGKIYKLC
                     GILSSLSNPETALEGFVNDYFQPKEKDSYIVYVKYDENKNIKKQNYKFMKKFNISKEK
                     TYINWPLSVEFGVKPPKNIEKYNIFEFLKNLELNTNAIILLVGVFGAAAVYSIFHVSI
                     LQRISQYGVLEVLGANNKQLLFLLFLELFLLFIVGFPIGCFLGIEVASTIYEQFPHIF
                     LSSDIVPGAFFISEKSIYLGFLFILLLLILIAVRVVHQLNKYSSIESMKNFKITNKKK
                     RILESVKHSNMTKVLSHKYMTQKKGMFIGILFSLSLGGIIFLCSSYSIQLTKNNNELT
                     MKADDGLNSDYQITMQTTDFDVGIPKDKISRLNNVEGISKVYPVRYLFGAMQIKEEQL
                     FWKNFFKPLEKDYRIKTFFDGICTKQNEGGYLLKTNIYGYNNGMLEGLNSYIIDGEIN
                     THDMVKNNKVIVRLPMDGTGMYDAVDINPGDTIKVKVPKTMKPTDEIVKFKEENKNDY
                     TTKEFVVAATVKRVMANNIYFIGDYGMDIVMTNGQMKNNFDIENYNSVSIKKTEDSNS
                     EELSENIKSVVSDVKRCIVTDYTIAIEKNNTYLNQKLLFIYGIVFVLLAISLFHIINT
                     VSYLIFSRRHEFGILRAMGITDNKFLLMMIREGFLYGFYASIIMVIGSVIGQFMIYFM
                     VKRVYLYINPILKINTPLYIGMIILNITISIVAVIIPVRQILKSDIISEINKN"
BASE COUNT         1982 a          506 c          810 g         1567 t
ORIGIN      
        1 tgaaggtctt ttttatgcat ttgagaatga tggatttaat gtatttaagg cttatacaaa
       61 acaagaagga ttaaatatat ttaatagtaa aaatatagat tttataatct tagactgcaa
      121 tcttccagat ggatatggat ttgatgtatg tcaaataata agggaaaagt cagatatacc
      181 aattataatg cttactgcaa gagactcaga aattgatgaa gtaaaaggat tagaaatagg
      241 gcttgatgat tatattacaa aaccattttc tttatctgta ttaaaggcaa gagtcaaagt
      301 agcaataaga aaaaaatcga ataataaagt aatatattcc aatggaataa aattggacca
      361 aaagttacta aaagtatata aaaataaaga atgtttggaa cttagctctg tagaatataa
      421 gcttctaagt tatctaattg aaaataaagg tcaaatatta ttaaaggaac aaattttaca
      481 ccatatttgg gacagcgaag aaaattatgt tgatgataat attgtatctg taaatataag
      541 aagacttaga gtaaaagtag aggatgaccc atctaatcca aaatatataa aaacagctta
      601 tggaatgggt tatttatgga atgaggtaga ataatatgtt tatattagcc tttataatat
      661 gtacagttgt tatatttata gtatctacta aaataaatcg aaaaagatat gatgaattaa
      721 atttgatgtt aaatcaaata ttggaaggta aagaagttac ttatccagat acaaaggata
      781 caagagcgtc aaaaatttct caccaagtaa aaaaaattaa ggatatgata gaaatagagg
      841 tagaacagtc aaagtcagaa aaagaagcga ttaaaggttt aatatcaaat atgtcacatc
      901 aattaaaaac tccactttca aatataacca tttattgtga acttctggag aatataaata
      961 tttccacatt acagaagaag gagtttttac aaaagatgaa aaatgaaact tttaagattg
     1021 actggctgtt acaatcctta tttaaaatga ctaaattaga ggatggagtt atagaatttg
     1081 aagtagaaga actactaatt aaagatactc ttattcaaag tatcagtact atctttaata
     1141 aagcagaagc taaaaatata aggataaatc tcgaaccatt ttcagacata aagttagttc
     1201 ataataaaaa gtggaccata gaagcaattg taaatgtttt agaaaatgca ataaaatact
     1261 caccatcaga tagcacgatt actatttctg taataaaaat ggaactatat acgaagatta
     1321 ctattaaaga tgagggaata ggtattgata gtagagaact aaatgatatt tttaaaagat
     1381 tttacagaag taaaaatgtg gaaaaccaaa atggcacagg gattggtcta tatcttacaa
     1441 gattaatttt agaaaaagaa aatggaaaca ttatagtaga atctaaacta ggaagtggat
     1501 gttgttttag tatcttctta cagaattgta agagtttaaa ctaattttgt aggatgagtg
     1561 taattatcgt cacttacaat attttataga acatatatct gtattgaaag gagaaattaa
     1621 atgagtatat tagtgacgaa taatttagta aaacactatg gaaaaggtga gactaaagta
     1681 aaagccttaa atggagtaag tatagaaatt gaacgagata catttactgc tatagttgga
     1741 acttctggtt ctggaaaaag cacattgtta aatattatag gtggattaga taatccaact
     1801 tcaggtgatg taataattaa aggaaagaac atatctaaaa taggaaaaaa agatttgaca
     1861 gtatttagaa gaagaaatat aggatttata tttcaaaact acagtttaat gcccgtatta
     1921 aacacttatg ataatatagc tcttcctgtt acattagata aaggaaacca tatagaccat
     1981 gaatatattg aaatgctgat gaagacattg ggaatttggg ataagcgttt aaaatttcca
     2041 agcgaactat ctggtggtca acaacagagg gtagcaattg ctagagcttt ggcaaataaa
     2101 cctgcgctta tattagcaga tgaaccaaca ggaaatctcg atagtaaaac tacaatggaa
     2161 gtggtatgtt tattaaaaga aagtagtgca aaattccatc aaacgatact tatggtaaca
     2221 cacaatgaaa atatagcaca agtatgcgac tcaattatcc atattgaaga tggagttgtg
     2281 gtgaatactg gtggtgaagt actatgatgt ctctagtaaa attttcaatt caatatataa
     2341 agcattataa aaaacaatct atatctatag ttttaagcat agtactatca gtagcattat
     2401 tgacgggaat tggttcttta gttcatagtg cagacaaaag tagaattgaa aagataaggg
     2461 aagatagtgg agattatcat tatttctata aagtagataa ggaacaactt aataaaatta
     2521 aagaaaataa aaaatcaaag gaatacactg tagatagact tggcattaca aaacctaaaa
     2581 atcagataga tgaacctttt attatagaat ttttaaatgc tgattcttca tatttagaca
     2641 tgacaggtag aaaattatta aaaggaactc ttcctagtaa agatggagag atagtacttg
     2701 acacatattc tctaaacaat ttaaatgtaa acaaagaaat tggaacagaa atcaatttag
     2761 atggtaaaat ctataagtta tgtggtattt tatcaagtct ttcaaaccca gaaactgctc
     2821 tggagggatt tgtaaacgat tattttcagc ctaaagaaaa ggattcttat atagtttatg
     2881 taaaatatga tgaaaataaa aatattaaaa agcaaaatta taaatttatg aaaaaattca
     2941 acatatcaaa agaaaaaact tatataaact ggcctctttc agttgaattt ggagtaaaac
     3001 caccaaaaaa tatagaaaaa tataatatat ttgagttctt aaaaaattta gaactaaata
     3061 caaatgcaat tatattactt gttggagtgt ttggtgcagc agcagtatat agtattttcc
     3121 atgtttcaat acttcagaga atatcacaat atggggtttt agaagtatta ggcgctaata
     3181 ataaacaact tttatttcta ctatttttag agttattttt actttttatt gttggatttc
     3241 ctataggttg cttcttaggt attgaagtgg cgtctacaat atatgaacaa tttccacata
     3301 tatttttgag tagtgatatt gttccaggag cattttttat aagtgaaaaa tctatttatt
     3361 taggcttttt atttatctta ttgttgttaa ttttaattgc tgtacgtgtt gtacatcagt
     3421 tgaataaata ctctagtata gaatctatga aaaactttaa gattactaat aagaaaaaga
     3481 gaatattaga aagtgtaaaa cactctaaca tgacaaaagt attatctcat aaatacatga
     3541 ctcaaaaaaa gggaatgttt attggaattt tgttttcatt atcacttggt ggaattatat
     3601 ttctatgttc tagttactct atacagctta ctaaaaataa taatgaactt acaatgaaag
     3661 ctgatgatgg tttaaattct gactaccaaa taactatgca aacaacagat tttgatgtag
     3721 gtataccaaa agataaaatt tcaagattaa acaatgttga gggaatatca aaagtctatc
     3781 cagtaagata tttatttgga gctatgcaaa ttaaagaaga acaactattt tggaaaaatt
     3841 tctttaaacc tcttgaaaaa gattacagga taaagacttt ttttgatggt atttgtacaa
     3901 agcaaaatga aggtggctat cttttaaaaa caaatattta tggttataac aatggtatgt
     3961 tagaaggatt aaattcttat attatagatg gggagattaa tacccatgat atggttaaaa
     4021 ataataaggt tatagttaga ctacctatgg atggaactgg tatgtatgat gcagttgata
     4081 taaatccagg agatacaata aaagttaaag tgccaaagac aatgaaacca acagatgaaa
     4141 ttgttaaatt taaagaagaa aataagaatg attatacaac aaaagaattt gtagttgctg
     4201 ctactgtaaa acgtgtgatg gcaaataaca tatatttcat tggtgattat ggtatggata
     4261 tagttatgac gaatgggcaa atgaaaaata actttgatat cgaaaattat aattcagtaa
     4321 gtataaaaaa gacagaagat agtaacagtg aggaattgtc tgagaacata aaaagtgttg
     4381 taagtgatgt aaaaagatgt attgtaacag attataccat tgctattgaa aaaaataata
     4441 cctacttaaa tcaaaaactc ttatttatat atggtattgt atttgtactt cttgcaataa
     4501 gtttgttcca tataatcaat acagtaagct acttaatttt ttcaagaaga catgagtttg
     4561 gaattttaag agctatgggt attacggata ataagttttt acttatgatg ataagagaag
     4621 gttttttata tggattctat gcaagtatta taatggtaat tggaagtgtt attggacaat
     4681 ttatgattta ttttatggta aaacgtgtat acttatatat aaatccaatt cttaaaataa
     4741 atacacctct atacataggt atgattattt taaatatcac tattagtata gttgctgtaa
     4801 ttattccagt aagacaaatt ttgaagagtg atattatttc agaaataaat aagaattaga
     4861 gttat
//
LOCUS       DAELLV010000250         4852 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.250, whole genome shotgun sequence.
ACCESSION   DAELLV010000250 DAELLV010000000
VERSION     DAELLV010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4852
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.250"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            521..1516
                     /locus_tag="KPD58_003234"
     CDS             521..1516
                     /locus_tag="KPD58_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0266965.1"
                     /translation="MSIIELNELTKSYRRFEKKAGFMGSVKSLFNRKYTEKTAISSFD
                     LKIEEGEFVGLIGQNGAGKTTLIKMLTGIIAPSSGEISVMGYYPNKLENNFKKQYAVV
                     MGQKSQLFFELTPADTFLLFKELYDISNEEYKKNLDYFIELFEVSDYLNVQVRTLSLG
                     ERMKMELIVALLHNPKILFLDEPTIGLDAIAQKQIRYFLKEVNETKGTTIILTSHYME
                     DIKSLCSRSIVVENSRKIYDGSTEKLFNYFQKYKKITVSFSNETNIVFPKDCKVVEQT
                     NHKTSVIVPKERGNETIKWIFDKYDVKDVMIEEEDIGSIVERIYKKGSDEYYEQL"
     gene            1494..2357
                     /locus_tag="KPD58_003235"
     CDS             1494..2357
                     /locus_tag="KPD58_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HBG0266966.1"
                     /translation="MSIMSNYNEASSNYSMFYKFKQSIRRYYEVAKINFKVQLIWRFD
                     VVVNMVFTIVKILFAYILWKAIFGENKIVSGFTFNSMLSYYIISSFLAGIDMSKSTSE
                     EICNRVKLGTFSKYMIIPINVQNYFIAKNFGMTCFYFVFNMISAFVWIFIFKIDFAFT
                     SNPVQILISILMIFLGFIFMMKLHFFIGILSFKFLEISFFRMIIENIVEFITGTMIPL
                     VLLPETIVTVMKVFPFYYTTYLPSMLLIGRNSNEAIQGLIILALWVLFFSVVNKVMYK
                     RMRTLFDGVGI"
     gene            2354..3148
                     /locus_tag="KPD58_003236"
     CDS             2354..3148
                     /locus_tag="KPD58_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HBG0266967.1"
                     /translation="MMKKNLKIIFMLLKMKLSKMMVFRFDFFGAFFVDSSLFILQLLM
                     FNSIYSHVDSIGGWKQGEMLIFIGTFSLINAINMTIFFFGIYDIPRKIQEGELDYYIT
                     KPLDPLFRLTFENINIGSSPLILASILIVLYGISELNSEITLAMIIIYIFMVLLMTLL
                     FYDVCIILRTVSFFVISSSAIMRLEDNLLPMNMKIPGVIYEGVFKVLFYLILPYGIMS
                     TIPTQILAGTITINGLIYSILIVFLFTLFMWRFWKLGMRHYKSASS"
     gene            3433..4812
                     /locus_tag="KPD58_003237"
     CDS             3433..4812
                     /locus_tag="KPD58_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG0266968.1"
                     /translation="MSDVYYSENHEQDNLYEQDNLLAQRFNLFTLLKFAFPTIFMMIF
                     MGLYTIVDTIFVSRFINTNALSAINIVCPIINLIVGIGTMLATGGSAIVARKMGNGDI
                     EGANKAFSLIVLSGVVIGVIITGIGIVFMDYIIIWLGASEVLIKYCRSYLFIILVFAS
                     ANILQVLFQCLFVTAGKPQFGLWLVLGAGCANIILDYVFIVLLQMGISGAALATGVGY
                     LIPTVSGMVFFSKNKGLLKFCIPRLDKRILFEACFNGSSEMVSQLSTAVTTFLFNAMM
                     MKLIGEDGVAAITIIIYSQFLLTSLYIGFSMGVAPIISYNYGSENNTQLNHVFKICIL
                     FISVASLLVFIFSISCSPIIVEIFSQRGSRVYEIARSGFYIFSFSFIFCGYNIFTSSM
                     FTALSNGKVSAILSFLRTFGFITIGLLILPNILNINGIWLAVPSAEILTLFLTIFCIC
                     KYKNRYKYM"
BASE COUNT         1725 a          487 c          794 g         1846 t
ORIGIN      
        1 taaacgaata ttataaatga aatttaaatt caaccttgaa gaaataagct aaatgtatta
       61 gtatataaat ccatgcttga acataaataa cctttatgat atgatatcaa taataatatt
      121 atgagaggag tgccttgatg tcggagttat ataatggtaa ccaacaatta aatagttgga
      181 gacacatata ttctgtataa tcagaaattt caagtgtatt tttaatacat ttaatttttg
      241 tgtcttattc tgcctttata gataactgac atatgacaac aaatagtatt taaaatatat
      301 attactttgt taaattatgg tgtagtaggc tttaatctgc tacactttta ttaattttgt
      361 atagtattat tttgataatt attttgaaat agagagcatt aatttttcta gtccacagga
      421 gttttctgtt tttgtgctgt taaatataag cacagaaaaa ggcagatata tacctgtgga
      481 ctttttttta tataaattat gaaatatgga ggtagttaaa atgagtataa ttgaacttaa
      541 tgaactgact aaatcttata gaagatttga gaaaaaggca ggatttatgg ggagtgtaaa
      601 gtctttattt aataggaaat atacagaaaa aacagctata tcgtcatttg atttaaagat
      661 agaagaagga gaatttgttg gattaatagg tcaaaatgga gctggcaaaa ctactcttat
      721 taagatgtta actggtatta ttgcaccgtc ttctggagaa atatctgtaa tgggttatta
      781 ccctaacaaa ttagaaaata attttaaaaa acaatatgct gttgttatgg gccaaaaaag
      841 tcaactattc tttgaattga caccagcaga tacgtttctt ttatttaaag aattatatga
      901 tatatctaat gaagaatata aaaaaaactt ggattacttt atcgaattat ttgaggtatc
      961 tgactattta aatgttcaag ttaggacgct ttctttagga gaaagaatga aaatggaact
     1021 aatagtagcc cttctacata atccaaaaat attatttttg gatgaaccaa ctataggctt
     1081 agatgctata gcccaaaaac aaattaggta tttcctaaaa gaagtaaatg aaacaaaagg
     1141 aactactatt atacttactt ctcattacat ggaagatatt aagagtctct gtagtagaag
     1201 tattgttgta gaaaatagtc gtaaaatata tgatggttct acagagaaat tatttaatta
     1261 ttttcaaaaa tataaaaaaa taacagttag tttttccaat gaaactaaca ttgtatttcc
     1321 aaaagattgt aaagttgttg aacaaacaaa tcataagaca tcagtaattg tgccaaaaga
     1381 aagaggaaat gaaacgatta aatggatttt tgataaatat gatgttaaag atgtaatgat
     1441 tgaagaagaa gatataggta gtattgtaga gagaatatat aagaaaggaa gtgatgagta
     1501 ttatgagcaa ctataatgaa gcaagttcaa attattcaat gttttataaa tttaaacaga
     1561 gtattagaag gtattatgaa gtagccaaaa ttaattttaa agtgcaactt atctggagat
     1621 ttgatgtggt ggttaatatg gtgtttacga ttgtaaaaat tttatttgct tatattttat
     1681 ggaaagcaat ttttggtgaa aataaaatag tctctggttt tacatttaac tctatgttat
     1741 cttactatat aataagctca tttttagcag gtattgatat gtcaaagagt accagtgaag
     1801 agatatgtaa tcgtgtaaaa ttgggcacct tttcaaagta catgattatt ccaattaatg
     1861 ttcaaaatta ttttattgct aaaaattttg gaatgacatg tttttatttt gtatttaata
     1921 tgatttctgc atttgtatgg atttttatat ttaaaataga ttttgccttt acatcaaatc
     1981 cagttcaaat attaatttca attttgatga tttttctagg gtttattttt atgatgaaac
     2041 tccacttctt tattgggatt ttgtcattta aatttcttga gattagtttt tttagaatga
     2101 taattgaaaa tattgttgaa tttattacag gaacaatgat accacttgta ctcttaccag
     2161 aaactatagt aacagttatg aaggtatttc cattttatta tacaacatat ttgccatcta
     2221 tgttactaat aggtagaaac agcaatgagg caatacaagg attaattatt ctagcattat
     2281 gggttttatt tttttctgta gtaaataaag ttatgtataa aagaatgaga acattatttg
     2341 atggggtggg tatatgatga aaaaaaattt gaaaataata tttatgttat taaaaatgaa
     2401 attatcaaag atgatggtat ttagatttga tttttttgga gcattttttg tagatagttc
     2461 attatttata ttacaacttc taatgtttaa tagtatatat tctcatgtag actcaatagg
     2521 gggatggaaa caaggagaga tgttaatatt tataggaact ttctcattaa taaatgcgat
     2581 aaatatgacg attttctttt ttggtattta tgatattcca cgaaaaatcc aggaaggaga
     2641 attagactac tatattacaa aaccattaga tccactattt aggttaacat ttgaaaatat
     2701 taatattggt tcatcaccat taattctagc aagtatactt attgttttat atggaatatc
     2761 tgagttaaac tcagagataa ctttagctat gataataata tatattttca tggtgttatt
     2821 gatgacatta cttttttatg atgtatgtat catacttaga acagtttcat tttttgttat
     2881 atcatcttct gcgataatga gattagaaga taatttattg ccaatgaata tgaaaattcc
     2941 tggtgtaata tatgaaggtg tttttaaagt attattctat ttaatcttac catatggaat
     3001 tatgtcgaca attccgactc aaattttggc tgggacaata acaattaatg gattaatata
     3061 ctcaatttta attgtatttt tgtttacatt gtttatgtgg agattttgga agttaggtat
     3121 gagacattat aaaagtgcaa gtagttaata aattagtaaa aatagacaat gtataactat
     3181 atataaatat gtttcatatg acactaaact atgtgaacaa gtatttgcgc taaaggcact
     3241 gaaagctata aaattattaa agtaaggatt taaaagtttc tttagtagaa atataattgt
     3301 gtaaaaaagc aagattaatt aaaactatat ccaaataatt agatataatt cttatgcaga
     3361 aaggaggtct gtaaatgtct aattgtttgg aatatatttt aaaaataatt aatgtacaga
     3421 aaaataaaca aaatgagtga tgtatattat tcagaaaatc atgaacaaga taatctttat
     3481 gagcaagata atttactggc acaaagattt aatttattta ccctattgaa gtttgcattt
     3541 cctactattt ttatgatgat ttttatgggg ttgtatacaa tcgtagatac tatatttgta
     3601 tcaagattta ttaatacaaa tgctctatca gcaattaata ttgtatgtcc aattattaat
     3661 cttatagttg gaattggaac tatgcttgcc actggaggaa gtgctattgt agcacgtaag
     3721 atgggaaatg gtgatattga gggagcaaat aaagcttttt ctttaatcgt attatcagga
     3781 gttgtaattg gtgtaataat tactggtata ggtattgtat ttatggatta tatcattata
     3841 tggttaggag caagtgaagt tttaattaaa tattgtcgtt catatctttt tattatcttg
     3901 gtttttgctt ctgcaaatat actacaagta ttatttcaat gtttatttgt aacagctgga
     3961 aagcctcaat ttggattatg gctagtgtta ggtgctgggt gtgctaatat aattcttgac
     4021 tatgtgttca ttgttctgtt acaaatgggt attagtggag cagcacttgc aactggagta
     4081 ggatacctaa ttcctaccgt ttcaggtatg gttttctttt caaaaaacaa agggttactt
     4141 aagttttgta tacccagact agataaaaga atattatttg aagcttgttt taatggttca
     4201 tctgaaatgg tcagtcaact atcaacagca gttactactt ttttatttaa tgctatgatg
     4261 atgaaattaa ttggtgaaga tggggttgca gctattacaa taattattta ttctcaattt
     4321 cttttaactt cgctttatat aggtttctca atgggtgttg caccaataat aagttataac
     4381 tatggaagtg aaaataatac gcagttaaat catgttttta agatttgtat attatttatt
     4441 tcagtagcat ctttattggt atttatcttt tctatatcat gttcaccaat cattgtagag
     4501 attttttctc aaagaggaag ccgagtttat gaaattgcaa ggtctggatt ttatatattt
     4561 tcttttagtt ttattttttg tgggtataat atttttactt cgtctatgtt tacagcatta
     4621 tcaaatggaa aggtgtctgc aatattatct tttctacgta catttggatt tataactata
     4681 ggtttattga tattgccaaa tatattaaat ataaatggga tatggttagc agtaccaagt
     4741 gctgagattt taactttatt tttaaccatt ttttgtatat gtaagtataa gaatagatat
     4801 aaatatatgt agatattaaa taaatagttt aggtagatat tttatgtttt aa
//
LOCUS       DAELLV010000251         4838 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.251, whole genome shotgun sequence.
ACCESSION   DAELLV010000251 DAELLV010000000
VERSION     DAELLV010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4838
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.251"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..144
                     /locus_tag="KPD58_003238"
     CDS             <1..144
                     /locus_tag="KPD58_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG0266969.1"
                     /translation="YKFIEKLDEFIHKKYNYDLTDEEKLYLTIHVERVVSKSIKSDIN
                     PNN"
     gene            218..2134
                     /locus_tag="KPD58_003239"
     CDS             218..2134
                     /locus_tag="KPD58_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG0266970.1"
                     /translation="MRKYEQLSKEIIANVGGKDNISSLSHCITRLRFKLKDESIANDD
                     VLKKMDGIVTVMKSAGQYQVVIGNHVPDVYEVVCDLAGLGGDTIDNSDSDAPKGLLNK
                     FIDIISGVFQPILGVLCAAGMIKGFNAVFTALGLYGETDGAYIMLNAIGDAMFQYMPI
                     ILGYTAAKKFKLKPFIGMLIGAVLCYSGIQLDTLASTGQPLYNLFTGTIFESPVYITF
                     LGLPVIAMNYTSTVVPVILIVLLVAKLEKVFAKIIPDVVKNFFVPMAVLLVSLPLGFM
                     VIGPIATYASNIVGNGFLALFNFSPILCGALTGLLWQVLVIFGLHWGLVPIAMSNLMT
                     MGFDTILVGAFVPSFAQTAVVAAMYFKLKDKKIKELCIPAVISGICGVTEPAIYGITL
                     PRKTPFIYSCVGGAVAGAVMGIMNVKSYVMGGLGIFGIPNYINPTTGDMKGVYATIIA
                     IIVAIVVGFVLTLFFWKDDAIVEISKDDNGQGVIEKNKREVVYSPIKGEVKALEQIQD
                     AAFSTGILGKGVAITPKEGKVVAPFDGTVMTLFPTKHAIGIISDNGCELLIHIGLNTI
                     QLEGKYFKSFVNQGDKIKKGQTLITFDIESISKEGYCLETPVVVTNYSDYVDIVENTQ
                     KHVNNSQKLITVLA"
     gene            2221..3654
                     /locus_tag="KPD58_003240"
     CDS             2221..3654
                     /locus_tag="KPD58_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBG0266971.1"
                     /translation="MALRKDFLWGGATAANQCEGAWNLDGKGPSCSDMCTGGSQKVSK
                     RITRVIEKGTFYPSHEAIDFYHRYKEDIALFAEMGFKVFRFSIAWTRIFPTGMEKEPN
                     EAGLKFYENVIDECLKYNIEPLITISHYEIPFAITEKYNGWVSRDVIDLYMNYCETIF
                     KRYKGKVKYWLTFNEINSATMPMGGYLSQGILNEGTTDFINQVDIPQLRFQGLHHQFV
                     ASAKAVKLAHEIDSNYQVGCMQIFATMYPYTCNPDDAVKTQRDSRVMNYFCGDVQVRG
                     EYPTYMNRYFGENNIEIKMEKGDLEILKEGCVDFYTFSYYMSTCVSSDSKEDNTSGNI
                     LGGVKNPYLKSSEWGWQIDPEGLRYALNEIYDRYRIPMMVVENGLGAYDKKESNGVIN
                     DDYRIEYLKAHIEQMKEAVEDGVDLFGYTPWGCIDLVSASTGEMAKRYGFIYVDKYDD
                     GSGDLSRIKKKSFDWYKQVIESNGERL"
     gene            4048..>4838
                     /locus_tag="KPD58_003241"
     CDS             4048..>4838
                     /locus_tag="KPD58_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0266972.1"
                     /translation="MKTIAVVTDGLSKLECFLNKNLEMIFKDKVMIKNYYLNKITEKE
                     LIKDDIILVMIDDRVLKIKKYVEDVSKIITINRSIKQKDIYKLFSLPEGIDVLVVNDN
                     KHTVLETISNLYNIGINHLNFIPYNPNEEYRNIKIAITPGESSLVPKYIEEVIDLGHR
                     YIDSSTFIQIIAKLKLDDKEITERLIKYTDEIISLYSGINTKYKELTIKADELNSIIN
                     LSNLGMAMVSDKGNIIICNNSLRDILDIQSNIIGKNIDELENENI"
BASE COUNT         1905 a          479 c          932 g         1522 t
ORIGIN      
        1 tataaattca tagaaaagtt ggatgaattt atacataaaa aatacaacta cgatttaaca
       61 gatgaggaaa aattatactt aactattcac gttgaaagag tggtaagtaa aagtattaag
      121 tcagatataa atcctaataa ttaggtttat atatattggg tggtgacatt aagttggcca
      181 aaccttcata tttcaaaaag aaaatggagg agaaaaaatg agaaagtatg aacaactatc
      241 aaaagaaatc attgcgaatg taggaggaaa ggataatatt agcagtttat ctcactgtat
      301 aacgaggcta cgttttaaat taaaagatga gtcaatagca aatgatgatg tgctaaaaaa
      361 aatggatggt atagttactg taatgaaaag tgcaggtcaa taccaagtag ttattggtaa
      421 tcatgttcca gatgtttatg aagttgtgtg cgatttggct ggattaggag gagatacaat
      481 agataattca gactcagatg cgccaaaagg attacttaat aagtttatag acataatatc
      541 aggagttttt caaccaatat taggagttct gtgtgctgct ggtatgataa aaggatttaa
      601 tgcagtattt acagcattgg gtttgtatgg agaaactgat ggtgcatata ttatgttaaa
      661 tgcaattgga gatgcaatgt tccagtatat gccaataatt cttggatata cagctgctaa
      721 gaaattcaaa ttaaaaccat ttattggaat gcttataggt gcagttttat gctattcagg
      781 tattcaatta gatactctag catctacagg tcaaccttta tacaatctat ttactggaac
      841 aatatttgaa tcaccagtat atataacttt cttaggatta ccagtaatag caatgaacta
      901 cacttcaaca gtagtaccag taattttaat agtattatta gtagctaagt tggagaaagt
      961 gtttgctaaa ataataccag atgtagtaaa gaatttcttt gtaccaatgg cagttttatt
     1021 agtttcttta ccattgggat ttatggttat agggcctata gctacctatg cttctaatat
     1081 agttggaaat ggatttttag ctttatttaa cttttcacca attttatgtg gtgcattaac
     1141 tggtttattg tggcaagtgc ttgtaatatt tggtctacat tggggattag taccaatagc
     1201 tatgtcaaac cttatgacta tggggtttga cacaatatta gtgggagcat ttgtaccatc
     1261 ttttgcacaa acagcagtag tagcagcaat gtactttaaa ttaaaagata agaagataaa
     1321 agagctgtgt atacctgcag ttatttcagg gatatgtggt gtaacagagc ctgcaatata
     1381 tggaataact ttaccaagaa aaacaccatt catatatagt tgtgtaggtg gtgctgtggc
     1441 aggagctgta atggggataa tgaatgtaaa aagttatgta atgggtggac ttggaatatt
     1501 tggtatacct aattatataa atccaacaac tggagatatg aaaggtgttt atgcaactat
     1561 aatagcaata atagtagcta ttgtagtagg atttgtttta acattatttt tttggaaaga
     1621 tgatgctata gtagaaattt ctaaagatga taatgggcaa ggcgttatag aaaaaaacaa
     1681 aagagaagta gtatatagcc caataaaagg tgaagtaaag gctttggagc agatacaaga
     1741 tgcagcgttt tcaacaggta tattgggaaa aggtgtagca ataacaccta aagaaggaaa
     1801 agtagtggca ccatttgatg ggactgtaat gactttattt ccaacaaaac atgcaatagg
     1861 aattatatct gataatggtt gtgaactatt gatacatata gggttaaata ctattcagtt
     1921 ggaaggtaaa tatttcaaat cttttgttaa tcaaggggat aagattaaaa aaggtcagac
     1981 attaataact tttgacatag aaagtatttc aaaagaagga tattgcttag aaacaccagt
     2041 agttgttact aattattctg attatgtgga tattgtagaa aatacacaaa agcatgtaaa
     2101 taatagtcag aaattgataa ctgttttagc gtaaaagaga gttctattaa ttaagaatta
     2161 tatatatgta tttttatttt aaaataaagt tactataaat aaattattgg aggtagaatt
     2221 atggcactta gaaaagattt tttatggggt ggagcaacag cagcaaatca atgtgaagga
     2281 gcatggaatt tagatgggaa aggtccttct tgttcagata tgtgtactgg aggttctcaa
     2341 aaagtaagta aaagaataac gagagttata gaaaaaggaa cattttatcc aagtcatgaa
     2401 gcaatagatt tttatcacag atacaaagaa gatatagctt tatttgcaga aatgggattt
     2461 aaggtattta gattttcaat agcatggact agaatatttc caactggaat ggagaaagaa
     2521 ccaaatgaag caggacttaa attttatgaa aatgtgatag atgaatgttt aaaatataat
     2581 atagaaccat taataacaat atctcactat gaaataccat ttgcaataac agagaaatac
     2641 aatggatggg tttcaagaga tgtaatagac ttgtatatga attactgtga aacaatattt
     2701 aaaagatata aaggaaaagt aaaatattgg ctgacattta atgaaataaa ttcagctaca
     2761 atgcctatgg gaggctactt atcacaagga atattaaatg aagggactac agattttata
     2821 aaccaagtag atatacctca acttcgtttt caagggttac atcatcaatt tgtagcaagt
     2881 gctaaggctg ttaagttagc tcatgaaata gattcaaatt atcaagttgg atgtatgcaa
     2941 atatttgcaa caatgtatcc ttatacttgt aatccagatg atgcagttaa aactcaaaga
     3001 gatagtagag taatgaatta tttctgtgga gatgtacaag tgcgtggcga atatccaact
     3061 tatatgaata gatattttgg agaaaataat attgaaataa aaatggaaaa aggagattta
     3121 gaaattttaa aagaaggttg tgtagatttt tatacattta gttattatat gtctacatgt
     3181 gtaagtagtg attctaaaga agataataca agtggcaaca tattaggtgg agtgaaaaat
     3241 ccatatctta agtctagtga atggggatgg caaatagacc ctgaagggct tagatatgca
     3301 ctaaatgaaa tttatgatag atatagaata cctatgatgg tagttgaaaa tggtttagga
     3361 gcttatgata aaaaagaatc taatggagtg ataaatgatg actatcgtat agagtattta
     3421 aaagcacata tagagcaaat gaaggaagct gtagaagatg gcgttgattt atttggatat
     3481 actccatggg gatgtataga tttagtaagt gcatctactg gtgagatggc caagagatat
     3541 gggtttatat acgtggataa gtatgatgat ggaagtggag acttgtctag gattaagaaa
     3601 aagagttttg actggtataa acaagtaata gaatctaatg gtgaaaggtt atagtatgta
     3661 gatactgttt gtacttagaa gagtagtaaa tagtgtttat aaatacaatt ttttataatg
     3721 catttaatag gtaaaagtga tatatgattt gtacaattaa cctataaaaa tagattaata
     3781 atataaagac ttataagcta taagtgctaa gtctgtaaat actattgagg aactttagaa
     3841 taaaattaag aaaattatta atttggcaca atttttgctt tatagaagtt taagaaatat
     3901 gatagatatt gcaactatga tatttatgga cataaaacta ataaaggaat atgttagtat
     3961 actaaatgta caattttttg tgttattaaa gtgaaaatca aatgtatatt ataaaagagt
     4021 agagtatatt ttaaaaggag taatcaaatg aaaacaatag cagtagtaac tgatgggttg
     4081 tctaaattag aatgtttctt aaataaaaat ctagaaatga tctttaaaga taaagtaatg
     4141 ataaaaaatt attatttaaa taaaataaca gagaaggaat taataaaaga tgacataata
     4201 ttggtaatga tagatgatag agtattaaaa ataaaaaaat atgtggaaga tgtctcaaag
     4261 ataataacta taaataggag cataaagcaa aaggatatat ataagttatt ttcattgcca
     4321 gaaggaatag atgtattagt agtaaacgat aataagcata ctgtattgga aacaatatct
     4381 aatttataca atataggaat aaatcatttg aattttatac catacaatcc caatgaagaa
     4441 tatagaaata ttaagatagc tataacgcca ggagagtcaa gtttagttcc aaaatatata
     4501 gaagaagtta ttgatttagg ccataggtat attgatagtt caacgtttat tcaaataata
     4561 gcaaaactca aactggatga taaagaaatt actgagaggc taattaagta tacagatgaa
     4621 ataattagtt tatattcagg aataaatact aaatacaaag aacttactat aaaagcagat
     4681 gaactaaatt ccataataaa tttatcaaac ttaggtatgg caatggtatc agataaggga
     4741 aacataatta tttgtaataa tagtttaagg gatatactag atattcaaag taatattata
     4801 gggaaaaata tagatgaatt agagaatgag aatattaa
//
LOCUS       DAELLV010000252         4806 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.252, whole genome shotgun sequence.
ACCESSION   DAELLV010000252 DAELLV010000000
VERSION     DAELLV010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4806
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.252"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..529)
                     /locus_tag="KPD58_003242"
     CDS             complement(<1..529)
                     /locus_tag="KPD58_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266973.1"
                     /translation="MSNQRKDFDITNDMFQESIPITTKIILEDMPSNDELNHVFSKGC
                     ERKMKKRKIVLITLLLIGVLLLGSILYNLFLGKTANISMLKESWNFDIPIPNKEIEVF
                     DTQDSINGDGQSYFIQGFSEKNFKKVFNLKGGIVVSKDNINEIEKYIDKFKRDSVNIN
                     KSNKNKIEEDFKKYKL"
     gene            complement(939..1115)
                     /locus_tag="KPD58_003243"
     CDS             complement(939..1115)
                     /locus_tag="KPD58_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266974.1"
                     /translation="MFRDKMDRCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(1169..1265)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            2061..2462
                     /locus_tag="KPD58_003244"
     CDS             2061..2462
                     /locus_tag="KPD58_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HBG0266975.1"
                     /translation="MNYKNRKRAYYQENSCMDNFICKNCGCLVVPEGAGTQHRNHCPH
                     CLHSLHVDIIPGDRAADCDANMEPIGVWVRKNGEWAIIHRCTRCGHLSSNRIAADDNS
                     VKLMSIALKPLAQPPFPLERIIEKLEKEETE"
     gene            2459..3526
                     /gene="rsgA"
                     /locus_tag="KPD58_003245"
     CDS             2459..3526
                     /gene="rsgA"
                     /locus_tag="KPD58_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBG0266976.1"
                     /translation="MNGMKQINLQDYGLNRNFITQSMEYYDLYLARIISQEKNFYQAV
                     CVNGVLFAEVSGKLRFEATIQSDFPTVGDFVMLDRCNNKNGNAIIHHVLHRKSLFVRK
                     AAGKVNEEQVVAANIDIVFLCMSLNKDFNIHRLERYLSLTWESGAIPVVVLTKSDLCD
                     NVHRKLQVVNSIALGVDVVVTSSIEPDGYKQISKYIKPGCTVALLGSSGVGKSSLINC
                     LMGGEFLNTKEIRIDDDKGRHATTRRELFLISGGGIVIDTPGMRELGMWDVSEGLDKS
                     FADIEAYFGKCHFRNCTHTSEPGCAIWEAIAHQELSPKRWESYRRLKAEAVFADNKSE
                     YLAKKEKKFKNISKINKTIRR"
     gene            4062..4748
                     /locus_tag="KPD58_003246"
     CDS             4062..4748
                     /locus_tag="KPD58_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0266977.1"
                     /translation="MKISSQYRSQYSFRYESNINNTRINESMVKKNETVGKDTYLSNI
                     MKQKQELNDRIRDLKYRQEVYTKKINDAIKNLCKSEIRETTNNFSNIEIGIKNSIIED
                     KNKSTMLDENSTYLNTNDEKESLITKESNEKIEEEILNDEKLEELEQKKDSNKKEKVS
                     EDLSLVGKTREELENMLKNFINLTQEEIMKLESRIEKLDKNAEEYKQNSKTNIFDKTD
                     EQKKHINVLI"
BASE COUNT         1832 a          556 c          710 g         1708 t
ORIGIN      
        1 ctaacttata ttttttaaaa tcttcttcaa ttttattttt attactttta ttaatgttaa
       61 cagaatctct tttaaattta tcaatatatt tttctatttc attaatatta tctttgctta
      121 caactatacc accttttaaa ttaaaaactt ttttgaaatt tttttcagaa aatccttgaa
      181 taaaataact ttgtccatct ccattaatac tatcttgagt atcaaatact tcaatttctt
      241 tgttaggtat tggtatatca aaattccaag attcctttag cattgaaata ttagctgttt
      301 ttcctaaaaa aagattatat aggatacttc ctaataacaa aacacctatt aatagtagtg
      361 tgattaatac aatttttctc tttttcattt ttcgttcaca accttttgaa aaaacatgat
      421 ttaattcatc atttgaaggc atgtcttcaa gaataatttt tgttgtgata ggaatgcttt
      481 cttgaaacat atcatttgta atatcaaaat cttttctttg attactcaat atccatttct
      541 tctaatattt cttttgattt tttctagtac attgaatact cttatataca ttctcctctt
      601 ttaaaattaa aagttataaa agaataagtt atataaaaaa ttattatatt ttttaatata
      661 gcttatttgc aaatttataa ttttgttaat ctaatccttc aaactaaaaa atcatttatg
      721 tttacagaat ttcattttaa gagatgttta ttttcacata gataagagaa tttaagttct
      781 catatttatt atttgataca aatttacatc tttattatat gtattttaaa taaaaaataa
      841 aattatgaat actagaaatc tatcttaatt agctttctaa cataaggcta tttaaagtag
      901 ataaaactaa aaagggttct ctagaaaacc aaatctattt aattatactt acttactaat
      961 accatcactt gatgcaaaca agattctacc acattttctc cgtactctag tataaaatca
     1021 tctagttgtg tatctataaa atcacaataa tcatatgaac taccaatata agcagtcaac
     1081 atatgtgtac atctatccat tttatctcta aacatatata ttattatccc cctaaaaaac
     1141 tttcataaaa acaaaaagaa tatctctttt tggcaactgg ctgacataac tcatagttta
     1201 ttttataagc gttagtccct atagctttgc gtcactaaat ttctctagtt ttgccgattt
     1261 agttttattc tacaattaca ataatacaat agaattgctt attattcaat atacttattt
     1321 gaaaattaaa taaatttaca aatatgaacg ttattttata tcaaaaaaat agactatgca
     1381 tgtagaatat atggaaaaac ttgattgtag gttttgtatg aaaagacagt tgttattaat
     1441 ttaagtgaga taaattgagc aataggcata aaatgagaaa acaaataata gcagttaatt
     1501 tttattatta atactaggtg aacattaaat tttaaaatac cactagattt tatagaataa
     1561 gaaaatgaga caaacataaa tatatgaata tattaaatta tacttgaaca tacttatatt
     1621 ttatgataaa atttcaataa taattattag tgaaggagtg tcttgcctga tgtcagagtt
     1681 atatataatg gtaaccaaca aataaatagt tggagacgca tatgggttct gtttaatcag
     1741 aatttttaag tgtatttttg atacatttga ttgttgtgtc ttactctgcc tttatagata
     1801 actaacatta gacaacaaat aataatatta catattactt tgttaaattg tggtgtagtg
     1861 ggatttaatc tgctacactt ttattaattt tacacagtat tattttgaat ataattatgt
     1921 tgaaatagaa gatattaaat tttctagtcc acaggggttt tctgtttttg tgtaggtaaa
     1981 tatgtatacc acataaaaag gcagatataa atacctgtgg actttttttg tagaaaatac
     2041 aagatacgga ggaaataaaa atgaattata aaaatagaaa acgagcttat tatcaggaaa
     2101 attcctgtat ggataacttt atttgcaaaa attgtggttg ccttgtagtt cctgaaggag
     2161 ctggtacaca acatagaaat cattgcccac attgtttaca tagtcttcat gtagatatca
     2221 ttcctggtga ccgtgctgca gattgtgatg ctaatatgga acctattgga gtgtgggtga
     2281 gaaaaaatgg tgagtgggca attattcatc gttgtacacg ctgtggacat cttagttcta
     2341 atcgtattgc agctgatgat aattcagtga aattaatgtc aattgcatta aaaccactgg
     2401 cacagccccc tttcccacta gaaagaatca ttgaaaagtt ggaaaaggag gaaacagaat
     2461 gaatggtatg aaacaaatca atttacaaga ctatggtcta aataggaatt ttattactca
     2521 aagtatggaa tattatgatt tataccttgc tcggattatc tcgcaggaaa agaactttta
     2581 tcaagctgta tgtgtaaatg gtgtactgtt tgcagaggtt tcaggaaagc tgcgttttga
     2641 ggctactata caatctgatt ttcccacagt tggtgatttc gttatgttgg atcgttgcaa
     2701 taataaaaat ggtaatgcta ttattcatca tgtgcttcat agaaaaagct tgtttgtgcg
     2761 taaggctgct ggaaaggtaa atgaggagca ggtagttgca gctaatattg acattgtttt
     2821 tttgtgcatg tcactgaata aggattttaa cattcataga ttggagaggt atctgtcttt
     2881 aacatgggaa agtggagcta tcccagtagt agtacttact aaatcagact tatgtgacaa
     2941 tgtgcatcgt aaattacaag tagttaattc tatagccttg ggtgtagatg tagttgttac
     3001 ctcatctata gagccagatg gttataaaca gatttctaag tatattaaac caggctgtac
     3061 tgttgcctta cttggttctt caggagttgg aaaatcttca ttaattaatt gtctgatggg
     3121 tggagaattt ttaaatacaa aggaaattcg catagatgat gataaaggaa gacatgccac
     3181 gactagacga gagctatttt taatttcagg tggtggtatt gttattgaca caccaggtat
     3241 gagagagttg ggaatgtggg atgtttcaga aggtttagat aaaagttttg cagatataga
     3301 agcttatttc ggaaaatgtc attttcgtaa ttgtactcac acctcagaac caggctgtgc
     3361 tatctgggaa gctattgctc atcaagaact ttcgcctaaa agatgggagt cctatcgtag
     3421 gcttaaagct gaagcagtat ttgcagacaa taaaagcgaa tacttagcaa aaaaagaaaa
     3481 gaaatttaaa aatatctcaa aaataaataa gacgattaga agataagtgt agtttttttg
     3541 ttcattctat gagtctatat actattaata tattaaattt gccatcaatt tgttacttta
     3601 atgtgatttt gatggcaaat ttggtttgtg gtattattta aagcaattta ttaatttttt
     3661 taataaagtt tgatttttta tatgatgata tatacatgtg ataatgtata catatttgtt
     3721 acgataggct cctttataaa gaggtcttat gtaagataag aaaaggcata tttatactaa
     3781 tacataatag agtatatgct tatgtatgat agagatgttt cttaagagca aaactgcaat
     3841 attgctaatt tataaagtaa aaatatatga attataaaat acaaataata tatttataaa
     3901 aattattgtt ttaatttata ataaaaattt tttgttgaaa ataatgctcg aacctataaa
     3961 actattaact aatatttgta aatgtatact tttgatgata aaatataatt aagttaagtt
     4021 ggtttttata aacataaatt tattttaagg aggtgaaata aatgaaaata tctagtcaat
     4081 atagaagtca atattcattt agatatgaaa gtaatataaa taatacaaga ataaatgaaa
     4141 gtatggttaa gaaaaatgaa actgtaggaa aagacactta tttatctaat attatgaaac
     4201 aaaagcaaga acttaatgat agaattagag atttaaaata tagacaagag gtttatacta
     4261 aaaaaataaa tgacgcaatt aagaacttat gtaaatcaga aataagagaa acaactaata
     4321 acttttctaa tatagaaata ggtattaaaa atagcattat agaagataaa aataaaagta
     4381 caatgttaga tgaaaattca acttatctaa atacaaatga tgaaaaagaa tctttaatca
     4441 ctaaagagtc taatgaaaaa attgaagaag aaatattaaa tgatgaaaaa ttagaagagt
     4501 tagaacaaaa aaaggattct aataaaaaag agaaagtatc tgaggactta tctttagtag
     4561 gtaaaactcg tgaagagctt gaaaatatgc ttaaaaattt tataaattta acacaagaag
     4621 aaataatgaa acttgagtcg agaatagaaa agttagataa aaatgctgaa gaatacaaac
     4681 aaaattcaaa gactaatata tttgataaaa cagatgaaca aaaaaaacat ataaatgtac
     4741 tgatttaaac aatatgtttt aaaaaaataa gtttattata taatgaaaat ataaaggtac
     4801 aatttc
//
LOCUS       DAELLV010000253         4730 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.253, whole genome shotgun sequence.
ACCESSION   DAELLV010000253 DAELLV010000000
VERSION     DAELLV010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4730
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.253"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     misc_binding    316..553
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            617..2536
                     /gene="thrS"
                     /locus_tag="KPD58_003247"
     CDS             617..2536
                     /gene="thrS"
                     /locus_tag="KPD58_003247"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBG0266978.1"
                     /translation="MIKVALKDGSIKEFENAISVMDVAKSISEGLARNVVAASVNGEV
                     VGLDHIIDTDCDLNLFKFEDKEGKEVFRHTSAHILAQAIKRLYPEAKLAIGPSIENGF
                     YYDIDLDHRLVPEDLEKIEAEMKKIAKEDLKIERFELPRNEALELMKEQGEDYKVELI
                     SDLPESEIISFYKQGDFTDLCRGPHLPSTKKVKAVKLQSVAGAYWRGDENNKMLQRIY
                     GTSFEKNKDLEEYLHLLEEAKKRDHRKLGKELGLFMIPEEGPGFPMFLPKGMELKNEL
                     LKFWREIHRKAGYIEIESPIILNRKLWETSGHWYHYKENMYTVKIDDEDYAIKPMNCP
                     GGLIYYNSQLHSYRDFPMRVAELGRVHRHELSGALQGLMRVRAFTQDDSHIFMLPEQI
                     KDEIKGVANLIDGIYKTFGFEYNLELSTRPENSMGSDEEWEAAENGLREALEELGLPY
                     TINEGDGAFYGPKIDFHLKDCLGRTWQCGTIQLDMQLPRQFDNTYIGQDGEKHRPVMI
                     HRVAFGSIERFIGILIEHYAGKFPVWLSPTQVKILPISDKFMDYANEVKKELFDKGIR
                     VELDDRAEKIGFKIREAQLEKVPYMLIVGEKEVADNNVSVRSRDKGEIGSIKLDEFIA
                     SISKEIESRESIIQD"
     gene            3304..4395
                     /locus_tag="KPD58_003248"
     CDS             3304..4395
                     /locus_tag="KPD58_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBG0266979.1"
                     /translation="MIEKVEEKSKIIKTSKEYKRTIIEEKDKEWTKKLNDILSRSSFS
                     NDELDKIKALIPKEILTNENVGEVVTEDGYAKPEYDEVFKSLFTLNNEYDLLVNFIND
                     ILKDAPYANRNIKPFTQIKRIIRVETDPTINYIGEKRPRLDILAEDEENNHINIEMQR
                     AFENDYLERAEYYLSRVHGRKLEEGKEYKEIGKTIGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIYSSKTALYFIELPKIHKSDYIVNRIMLWGKLISNPSSLDVRVIAKNDSIIK
                     KALDRLKELGSNEEYLLNLKRGAYIMNKSRNFKEEIERETAIRVAKEKDYKIIIMLKK
                     NGFKLDDILNKFNDFDLSEDEIKEVYENN"
BASE COUNT         1864 a          473 c          846 g         1547 t
ORIGIN      
        1 cttattaact atattaagta ttgattgtat ttaagtatac aaaacctaaa aagagtacaa
       61 aaattagaag ttagttttaa taaaaattag ttttagtatt tatctagtca tatctatcat
      121 gcatgtggat acgttttaaa aattttaaat ttatgaaaat attataatat cactataaat
      181 caagagatta atttaatata aaaatataag aatattggtt tacagctgat tgagatgaaa
      241 aataatgggt aattaatata ttacattaat gttgattgat gtataactat ctgttataat
      301 gaaaatatat taaaaatcat agtgttgaag aggaagagta gagtagaaac cattctcaga
      361 gaatttgggt ctatggctga gagcccatgt aataggtcta tttgaaaact accttggagc
      421 tgagcttatt agcagtattt gttttgtact atacgtgaaa gtgctcgggt gacgacctta
      481 tagtcatcaa gagggttgta tatacttgta tacgccaact tgggtggaac cgcgaattaa
      541 tatcgtccca aacatatttg gggcgatttt ttattacaaa aaatctaaaa attatttcaa
      601 taaggaggaa aaaacaatga ttaaagttgc tttaaaagat ggctcaataa aagaatttga
      661 aaatgctatt tctgttatgg atgtagctaa atccattagt gaaggattag ctagaaatgt
      721 ggtagcagcg tctgtgaacg gagaggttgt aggtctggac cacattatag atactgattg
      781 tgacttgaat ttatttaagt ttgaagataa agaaggtaag gaagttttta gacatacatc
      841 agcgcatata ttagctcaag ctataaaaag actttaccca gaagcaaagc ttgctatagg
      901 accaagtata gaaaatggat tctactatga tatagattta gaccatagat tagttccaga
      961 ggacctagag aaaatagaag ctgaaatgaa aaaaatagct aaagaagatt taaaaataga
     1021 aagatttgaa cttcctagaa atgaagcttt agagcttatg aaagaacaag gagaagacta
     1081 taaggtagaa cttatatctg atttacctga aagtgaaata atatctttct acaagcaagg
     1141 tgattttact gatttatgta gaggaccaca tttaccatct actaaaaagg taaaagctgt
     1201 taaattacaa agtgtagcag gtgcatattg gcgtggtgac gaaaataata agatgcttca
     1261 aagaatatat ggaacttctt ttgagaaaaa caaagactta gaagagtact tacatttact
     1321 agaagaagct aaaaagagag accacagaaa attaggtaaa gaattaggat tatttatgat
     1381 acctgaagaa ggtccaggat tccctatgtt tttaccaaaa ggtatggaac ttaaaaatga
     1441 attattaaaa ttctggagag aaatacacag aaaagctgga tatattgaga tagaatctcc
     1501 aatcatatta aatagaaaat tatgggagac ttcaggacac tggtatcatt acaaagaaaa
     1561 tatgtataca gttaaaatag atgatgagga ttatgctata aaacctatga actgtcctgg
     1621 tggattaatt tactataact ctcaactaca ttcatataga gatttcccaa tgagagttgc
     1681 agagcttggt agagttcata gacatgagtt atctggtgca ttacaaggat taatgagagt
     1741 tagagcattt actcaagacg attcacatat attcatgtta ccagagcaaa ttaaggacga
     1801 aataaaagga gtagctaact taatagatgg tatatacaaa acttttggat ttgaatataa
     1861 tttagaatta tctacaagac cagagaactc tatgggaagc gacgaagagt gggaagcagc
     1921 agaaaatgga ttaagagaag ctttagaaga gttaggtcta ccatatacta ttaatgaggg
     1981 tgatggagca ttctatggtc ctaagataga cttccatcta aaagattgtt taggaagaac
     2041 ttggcaatgt ggtactatcc aattagatat gcaacttcca agacaatttg ataatactta
     2101 tattggtcaa gatggtgaaa agcatagacc agttatgatt catagggttg cttttggtag
     2161 tatagaaaga tttataggta tattaataga gcattatgct ggtaaattcc cagtttggtt
     2221 atctccaact caagttaaga tacttcctat atctgataaa tttatggatt atgcaaatga
     2281 agttaagaaa gagttatttg ataagggtat aagagttgaa ttagatgata gagctgaaaa
     2341 aattggattt aagataagag aagctcagct tgaaaaagtg ccatacatgt taatagtagg
     2401 tgaaaaagaa gtagctgata ataatgtttc tgttagatca agagataaag gtgaaatagg
     2461 tagtattaaa ttagatgaat ttatagctag tatttcaaaa gaaattgaat caagagaaag
     2521 tattattcaa gactaatata tattatttta gtagctaaga tattgttata taagataagt
     2581 ttaatcaata tgttgatatg ttgatgcata gtgattaaat ttatgaaaag atgagtataa
     2641 ttagaggtac agttaatcat atttttatga ttaatgtacc tctagttttt tgtatggtag
     2701 atttattaat aaatatatca tagaagtgtt ttgaacatgg aaatatttat gtgcattctt
     2761 taaataaaag tttatttttt gagtgataat taagtgcttg ttggtaaggt tatggttgta
     2821 aaaatcataa ctttattttc atatttgttc cattttcata aaaattatta tccctttaag
     2881 aaaatttctt aatcctcttg aaatatttcc tttttagtct aaagttaaat ttctttcttc
     2941 aaaatgaata tttaaatgaa ctaaaattta agtacatttt ctaatcttat gagtctattt
     3001 ttcatagata attgccctat aatagaagaa tctatgttgg agctatgtgg tgacaataac
     3061 agtattgtca gtgattaatt aatgctttat aaatgattaa ttagtgtttt atgttatttt
     3121 tggacttacc ctctatacat agattaattt atttaacttt agatagagag agaatgtcaa
     3181 gtacatacac agacattaga gtttaagaaa gtgtttatgg agcaagaact tttaaaagag
     3241 gtagtgactt agtttaattt caagaaatga taaatacaaa agaggaaaaa gagaggtgtt
     3301 gaaatgatag aaaaagtaga agaaaaatct aagattatta aaacatcaaa agaatataag
     3361 cgaactataa ttgaagaaaa agataaagaa tggaccaaaa aattaaatga tatacttagt
     3421 agaagtagtt tttctaatga tgaattagat aaaatcaagg cgttaattcc aaaagagatt
     3481 ttgactaatg aaaatgttgg agaagttgtg actgaagatg gatatgctaa gcccgagtat
     3541 gatgaggtat ttaaatcatt gttcacttta aataatgaat atgatttact tgtaaatttt
     3601 ataaatgata tcctaaaaga tgctccatat gctaacagga atatcaaacc gttcactcaa
     3661 ataaagagaa taattagagt tgaaactgac ccaacaatta attacattgg agagaaaaga
     3721 cctagacttg atattttggc tgaagatgaa gaaaataatc atataaatat tgagatgcaa
     3781 agagcttttg agaacgatta tttggaaaga gctgaatatt atcttagtcg tgtacatggt
     3841 agaaagttag aagaaggtaa agagtataaa gaaattggaa aaactattgg aatacatata
     3901 ctaaatcatg taaaatataa tcacatagaa gattatgtaa attgcctgag attaacaatg
     3961 gacgggcatc cagatattta ttctagtaaa actgcattat attttataga gcttccaaaa
     4021 attcataaat ctgactatat agtaaataga attatgctat ggggaaaact tattagtaat
     4081 ccttctagct tagatgtaag agttatagct aagaatgatt ctattattaa aaaagcactt
     4141 gatagattaa aagaattagg tagcaatgaa gaatatttat taaatctgaa aagaggtgct
     4201 tatatcatga ataaaagtag aaattttaaa gaagagattg aaagagagac agcaattaga
     4261 gttgcaaaag aaaaagatta taaaataatt ataatgttga aaaaaaatgg ttttaaatta
     4321 gatgacatat taaataaatt taatgatttt gacttaagtg aagatgagat aaaagaagta
     4381 tatgaaaata attgatgata aaaagtgaac ttatataagt ttgctgaatc ggagtctgga
     4441 aatatttatg tgtatttttt aatgaataaa aatttatttt aaaaacaata atttagaggt
     4501 acagttaatt atatttttat gatactgtac ttctagtttt ttctattgta gatttattat
     4561 ttactaataa atctacaata gaagtgcttt tgttgtaaga atcattttat ctaatgattg
     4621 ttttttataa tagttaaatt tccatttgta gtgtttaaat ataaatctat aacagcattt
     4681 ctataaatgt tataaatcac tataatgatt caactcaaag ttactgctta
//
LOCUS       DAELLV010000254         4709 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.254, whole genome shotgun sequence.
ACCESSION   DAELLV010000254 DAELLV010000000
VERSION     DAELLV010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4709
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.254"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..707)
                     /locus_tag="KPD58_003249"
     CDS             complement(<1..707)
                     /locus_tag="KPD58_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBG0266980.1"
                     /translation="MFAEERIQAILNILKKEGRVTVKELSSKFNVTEDCIRKDLKNIE
                     KISSIRRIYGGAVLARETLENQDTKDRKEINIPTKKIIAEKAFNLIEDRETIFLDIST
                     INILLAKLLAESNKKVTLVTNMLDILNVVTSKPNNLNIISTGGLLNLSLDGFVGTPTI
                     EFISKYKFDKTFMGSCGVDVFNSSLTTFEIEDGLTKKAIINSSKKVFIVMEDKKFKFD
                     GNFKFAHLEDISSIITE"
     gene            981..1655
                     /gene="fsa"
                     /locus_tag="KPD58_003250"
     CDS             981..1655
                     /gene="fsa"
                     /locus_tag="KPD58_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBG0266981.1"
                     /translation="MQLILDTGNVEEIKELCTCLPIDGVTTNPSIVSKEKKNFKQLIN
                     EIGEIIGEDMPIHAQVLSTKYEEILEEALYISSLRKNIYVKIPVTQDGLRAIKDLHRK
                     GIKITATAIFTAHQGFLAAKAGANYIAPYVNRLDNISGDGVAMVSELIKIIDTYKMDT
                     KVLAASFKNAQQVIELMQHGVHSVTVPYDICKSMMNHPLTDWSVDKFIEDWENTFGKG
                     SKTNNI"
     gene            complement(1777..3170)
                     /locus_tag="KPD58_003251"
                     /pseudo
     CDS             complement(1777..3170)
                     /locus_tag="KPD58_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905757.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
     gene            complement(3248..4594)
                     /locus_tag="KPD58_003252"
     CDS             complement(3248..4594)
                     /locus_tag="KPD58_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG0266982.1"
                     /translation="MKDLTTGHEGKSIFFFAMPMLIGSLFQQLYNTADSIIVGRFIGK
                     EAMAAVSGANPIMFLLVAALMGVSLGFSILVSQFYGSGDLKKVKATIDTTYILLFIGS
                     ILISILGIVFGGPMLKLMNTPESVFAQSKLYLTIIFSGILFSAGYNSVSAILRGLGDS
                     VTPLYFLIIATILNIVLDLTFIVVLRMGVEGVALATIMAQAVSFIISIIYLNKKHEVL
                     KFKIKGIVYDNKIFKDGLRLGLPSGVQQMLFSIGNMALQFLVNSYGTSAMAAFGAGLR
                     IENFISLPIMNLGSAVSTFVAQNIGAGENERVKKGIRESIKMTLVLAVTVIALILLFR
                     ENLIALFNTDKDVIKIGSSYLFIIGPFFLFIGTSFVLSSAMKGAGDSMFALISSIVSL
                     WLGRLPASYMLSKFFGTDGIWMGIPFGWTLGLIVTVIYYKKGHWKTKAIVNHRINE"
BASE COUNT         1823 a          664 c          613 g         1609 t
ORIGIN      
        1 ttttctgtaa ttatagaact aatatcttct aaatgagcaa acttgaaatt tccatcaaat
       61 ttaaactttt tatcttccat aactataaat acttttttac ttgaatttat tatagccttc
      121 ttcgtaagac catcttcaat ttcaaaagtt gtaagtgaag agttgaatac atctactcca
      181 caacttccca taaaagtctt atcaaactta tatttcgata taaactctat agttggagta
      241 ccgacaaaac cgtctaaact gagatttaat aaacctccag tggaaattat atttaaatta
      301 tttggttttg aagtaactac atttagtata tccagcatat ttgtaacaag tgttactttt
      361 ttattacttt cagctaacaa ctttgcaagc aagatgttaa ttgtagagat atctaaaaat
      421 atagtttctc tatcttcaat taaattaaat gctttttctg ctattatttt ctttgttgga
      481 atattaattt cttttctgtc ttttgtatcc tgattctcta gtgtttctct agctagtaca
      541 gctcctccat aaattcttct tattgaagaa atcttttcta tattttttaa atcttttctt
      601 atacaatctt ctgtaacatt aaatttagaa ctgagctctt tgacagtaac tctaccttct
      661 ttttttagga tatttaatat agcttggatt ctttcttctg caaacatttt ctacctcttt
      721 tcgcaatata gtattatagt aaatattata agataaaata caatatatca actattttaa
      781 catgaaatac aaaattaatt taatataaaa agtatgtttt ttctaactat atatttagaa
      841 tttagtaaat aaaaaaatta taaaactaag aaggaaaagt aaaacaataa taaaaaataa
      901 aaaacaataa gcaaaatgtt gaaaataata ccaaatcaat atataataag aataaagaaa
      961 gattaaaggg ggatatttta atgcaactaa tcttagatac aggtaatgtg gaggagatta
     1021 aggagttatg tacttgttta ccaatagatg gtgtaactac aaatccaagt attgtttcaa
     1081 aagaaaaaaa gaattttaag caacttataa atgaaattgg tgagattata ggtgaggata
     1141 tgcctataca tgctcaagtg ttaagtacaa agtatgaaga aatattagaa gaagcattat
     1201 atataagtag tttgagaaaa aatatatatg taaaaatacc tgtaacacaa gatggtttaa
     1261 gagctataaa agaccttcac agaaaaggta taaaaataac tgctactgca atattcactg
     1321 ctcatcaagg ttttttagct gcaaaggctg gagcaaatta tattgcacct tatgtaaata
     1381 ggcttgataa tatatctggt gatggagtag ctatggtttc agaacttata aaaataatag
     1441 atacttataa aatggataca aaagtacttg cagccagttt taaaaatgca caacaagtta
     1501 tagaattaat gcaacatggg gtgcatagtg ttacagtacc atatgatatt tgtaaatcta
     1561 tgatgaatca tccattaact gattggagtg tagataagtt tatagaggat tgggagaata
     1621 cttttggaaa aggaagtaaa acaaataata tttagtatta tggtaaaaga gtagttttgt
     1681 ccaatttcac aagaacttat ataaaaagat gaaaatatga attttctttt taataatttc
     1741 gttattatct taagttaatt catattttca tcttttttag atttctagtc ctataaattt
     1801 ccaccaaggg aatgttatac atattattat aaagtaggtt aaaggaatca aagttaaacc
     1861 gtatttgaaa ataaccttgc tattatacag attttcacca tgaccaacca ttattgtagc
     1921 atgatgaaat ggtaatatgt actgtatgct tactataatg tagacaaata gacataaagt
     1981 aacactattt tcctttagta tatgtaaact tccaagtaac ggtattacta cagatgatgt
     2041 agtgacagca cttccaagac atatattcaa gattattgtg agagttatca aaaacaatat
     2101 tgaaataatt tctgaagagt ttggtataaa ctttatcaaa taatttccca ttatatctac
     2161 aactccagag tactttaaca ctcctccaat tgaaaatgct gctataaaaa aaataaggac
     2221 cttccaattt atttctttta aagtactaaa tccaactatt tttcttagta ataaaagaag
     2281 tacacaaatt gacattataa ttaatgtgtt tatgttatgt atgaactgag ttaaaaacat
     2341 aaaaaacatt attcctaggc ataaaaatac gtgtttttgc tctttgttta gcaattaaat
     2401 tcttttttta aattagaata tgaaaaatga agtttattac cctcaagatt ttcattagaa
     2461 ataaatttta ttgaatgcaa ttctttttta aagacaaaga taaataaaaa ataagttata
     2521 aaacaagtaa ttatagttgg aacagacata taaaaagccc attgacccca atttatatta
     2581 caatttccaa gttctagtac aacataattt agaagagtgt ctccatttac aaaaaacatt
     2641 gatgtaaagg tagatgctgt aaatatacta aataaaagta tagattttga atcttcagtt
     2701 atctgttgtt cttttaaaaa ctccttgtaa aaagcagata caagaataac ccttggaaat
     2761 ggttgtggga taaaaaatat taaaagtata cctaaaatat acgataaaag aatcattttt
     2821 ttaggggtat ttacagtatt taaaattaac ttctcagaaa taatttttgc gacacctgtt
     2881 tttgtaactg cattagtaat tatgtatgca agaattatta tgtaaaaatt ttctgtaaat
     2941 aaaaactgta aaataacctt tattggtgca atgttaaata taaacgctag aattacaaaa
     3001 aacaatgcaa caaaagtttt ttcaactgag tctacagccc atatcaagat agttaaaaaa
     3061 gataagcata tagaaaattt tatgttgaaa ctgtaattaa gagagaagta gagaattatg
     3121 gtagctataa cagtcatata aaattgtttt ggcttaagaa taatacccat ttttatattc
     3181 acctccataa tttattatat cataatggag gtgaatacaa tgaatgtgaa tacattttat
     3241 aaaaagatta ttcattgatt ctatgattta caatagcttt agttttccaa tgtcctttct
     3301 tatagtagat aactgtaaca ataagaccta aagtccaacc aaaaggaata cccatccaaa
     3361 ttccgtctgt tccaaaaaat ttagaaagca tataagaagc tggaagtctt ccaagccata
     3421 aagatactat tgagcttata agtgcaaaca tggaatcacc agcacctttc attgcacttg
     3481 aaagtacaaa agaagttcca ataaataaga agaatggtcc tattataaat aagtaagaag
     3541 agccaatttt aatgacatct ttatcagtat tgaatagagc aattagattt tccctaaata
     3601 gcaaaattaa agcaataact gtaactgcta aaactaaggt catttttata gattctcgaa
     3661 tgcctttttt aactctttca ttttctccag cacctatgtt ttgagcaaca aaagtagaaa
     3721 cagcagaacc aagattcata ataggtaagc ttataaaatt ttctatccta agaccagcac
     3781 caaaagctgc catagcagat gtaccataac tattaactaa aaattgaaga gccatatttc
     3841 caattgaaaa caacatttgt tgaactccac ttggaagtcc taatctaagt ccatctttaa
     3901 atattttgtt atcatataca attcccttga ttttaaattt aagcacttca tgttttttat
     3961 ttaaataaat tatacttatt ataaatgata ctgcctgagc cattatagtg gcaagtgcaa
     4021 cgccctcaac acccattctt agcactacaa taaaagttaa gtctaaaact atatttagaa
     4081 ttgtggctat tatcagaaaa taaagtggag ttactgaatc tccaagtcct ctaagtattg
     4141 cacttactga attatatcca gcagaaaata atattccact aaatattata gtaagataca
     4201 attttgattg agcaaaaacg gattctggtg tattcataag cttaagcatt gggccaccaa
     4261 atactattcc aagaatactt attaatatag aaccaataaa taaaagtata tatgtagtat
     4321 caatagtagc ttttaccttt tttaaatctc ctgagccata aaattgtgac actaaaatag
     4381 aaaatccaag gctaacaccc ataagcgctg ccaccaataa aaacattata gggttagctc
     4441 cagagactgc cgccattgct tcttttccaa taaatctacc aactattata ctgtcagctg
     4501 tattgtatag ttgttgaaat aaactaccaa taagcatagg catggcaaaa aagaatatgg
     4561 actttccctc atgacctgta gttaaatctt tcacattatt cctccaaata aaaaaattat
     4621 taatttatta tagtataatc ttaacataaa ctttatatcc aaactataaa taaattgtat
     4681 caaaatatca tatatttttt taaaatgta
//
LOCUS       DAELLV010000255         4685 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.255, whole genome shotgun sequence.
ACCESSION   DAELLV010000255 DAELLV010000000
VERSION     DAELLV010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4685
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.255"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(103..1290)
                     /locus_tag="KPD58_003253"
     CDS             complement(103..1290)
                     /locus_tag="KPD58_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG0266983.1"
                     /translation="MNITKNFLKDYLEKFGETPFLVRLKDEEEFLIGEGSPQFKVIVN
                     NDISKSELLKSTSLALGEAYINRDIEVEGDLFLTLNLLLKQIDKFNIDNSAVKHLLHS
                     SNSMRHQKKEIHAHYDIGNDFYSLWLDKTLSYSCAYFRNDNDTLYDAQVNKVHHILTK
                     LNLEEGMSLLDIGCGWGFLLIEAAKKYKIHGVGITLSEEQFKEFNARIKEENLQDYLE
                     VRLMDYRELKKSDLMFDRVVSVGMLEHVGRENYELFIKNVNAVLKDGGLFLLHYISGL
                     QESYGDPWIKKYIFPGGVIPSLREMIHICSDYKYHTIDVESLRLHYVKTLLYWQKNFS
                     ENLPKIKEMFDDKFIRMWDLYLCSCAAAFNTGIIDIHQILFTKGIKNDLPLTREYMYN
                     CSK"
     gene            1563..2387
                     /locus_tag="KPD58_003254"
     CDS             1563..2387
                     /locus_tag="KPD58_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBG0266984.1"
                     /translation="MEVYALVGSSGTGKSFKALEFAYENDIEYIIDDGILIYKNKVLA
                     GISAKQANTTIEAVKRAIFYNLEHRQEVREAIKKENIRKILIIGTSKKMVNQIVERLS
                     IGRVCKFINIQDISTKYEIEIAKQARKEGNHVIPVPAVEIKSMASGLSINSLKRLFRK
                     GNNRNMVVLEKTIIRPTFSYIGKFYISADVIKQIIEYEVYNFGSIDRINKINIENCNN
                     FMNIFVSININDLETIKKIEDIQQTIKKSIEKMTLVNVQKIDINIHKIKKINFLQC"
     gene            2516..3781
                     /gene="gluD"
                     /locus_tag="KPD58_003255"
     CDS             2516..3781
                     /gene="gluD"
                     /locus_tag="KPD58_003255"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-specific glutamate dehydrogenase"
                     /protein_id="HBG0266985.1"
                     /translation="MSGKDVNVFEMAQSQVKNACDKLGMEPAVYELLKEPMRVIEVSI
                     PVKMDDGSIKTFKGFRSQHNDAVGPTKGGIRFHQNVSRDEVKALSIWMTFKCSVTGIP
                     YGGGKGGIIVDPSTLSQGELERLSRGYIDGIYKLIGEKVDVPAPDVNTNGQIMSWMVD
                     EYNKLTGQSSIGVITGKPVEFGGSLGRTAATGFGVAVTAREAAAKLGIDMKKAKIAVQ
                     GIGNVGSYTVLNCEKLGGTVVAMAEWCKSEGSYAIYNENGLDGQAMLDYMKEHGNLLN
                     FPGAKRISLEEFWASDVDIVIPAALENSITKEVAESIKAKLVCEAANGPTTPEADEVF
                     AERGIVLTPDILTNAGGVTVSYFEWVQNLYGYYWSEEEVEQKEEIAMVKAFESIWKIK
                     EEYNVTMREAAYMHSIKKVAEAMKLRGWY"
     gene            3994..4584
                     /locus_tag="KPD58_003256"
     CDS             3994..4584
                     /locus_tag="KPD58_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HBG0266986.1"
                     /translation="MHFKSKDLNLKYLVLFLKIVYWFNPFICIMEKYMDLDCEFYCDE
                     RVLKNCTVQQKQEYALTIVNSMKRGTNFSNKFVAGLNKQSDIIKRVMNMFSEKHKNGI
                     LMALIISLFSSITFFKFDSTSIIEENDFLERLRYTFEGRQEDMFVTIDFTYADTPIKL
                     RKEHEENCRVNGIVPKDSDSIEIIPRFYKDCMDLKK"
BASE COUNT         1769 a          541 c          777 g         1598 t
ORIGIN      
        1 caataaatag agttattcta aaataagaaa aatgacaaat cttatttaga aatagctcta
       61 ttttttgtaa ttaaatttaa attatattca atttgtaaaa tattattttg aacaattata
      121 catatactct ctagttagag gtaaatcatt ttttatacct ttagtgaaaa gtatttgatg
      181 tatatctata ataccagtgt tgaaagcagc agcacaagaa caaaggtata aatcccacat
      241 tcttataaac ttatcatcaa acatttcttt aatctttgga agattctcag agaaattttt
      301 ttgccagtat agtaaggttt ttacataatg taatcttaaa ctttctacgt ctatagtatg
      361 atatttgtaa tcactacaaa tatgtatcat ttctcttagg ctaggaataa ctcctcctgg
      421 gaaaatatat ttttttatcc aagggtctcc ataagattcc tgcaatccac ttatatagtg
      481 aagtaaaaac aatccaccat cttttaaaac agcatttacg tttttaatga aaagttcata
      541 gttttctcta ccaacatgtt caagcatccc aacgcttaca actctatcaa acataaggtc
      601 tgattttttt aattctctat agtccataag tcttacctca agatagtctt gaaggttctc
      661 ttctttaatt cttgcgttaa attccttaaa ttgctcttcg ctaagtgtaa ttccaacgcc
      721 gtgaatttta tatttttttg cagcttctat tagtaaaaat ccccaaccac aaccaatatc
      781 gagaaggctc ataccttcct ctaaatttag tttagttaat atgtggtgaa ctttattgac
      841 ttgagcatca taaagagtat cattatcatt tctaaagtat gcacaagaat aactaagtgt
      901 cttatctaac cataagctat aaaaatcgtt gccaatgtca tagtgggcat gtatttcttt
      961 cttttgatgt ctcattgaat ttgatgagtg caatagatgt tttacagctg aattatcaat
     1021 attgaatttg tctatttgtt ttaaaagtag atttaaagtt aagaataaat ctccctcaac
     1081 ttcaatgtct ctatttatgt aggcttctcc aagtgctaaa gatgtacttt ttaatagttc
     1141 agacttgcta atatcattat ttactataac tttgaactga ggggagccct ctccaattag
     1201 aaattcttcc tcatctttaa gcctaactag aaatggtgtt tcaccaaatt tctctaaata
     1261 gtcttttaaa aaattttttg taatattcat attaccagct ccttctaatg taaatccagt
     1321 aagtttccct gcttaaaata tattatagtc ctttttttaa aaaagtaaaa ttttttcaat
     1381 aaatttttta tttaaaggaa atttattttt tgtaaagaaa tttcatgttg tagtatattt
     1441 tttgcttata aattttggta ctggatataa tattaaactg ttaatgatat aataaattta
     1501 atctataaag taaataaaat tttgtaataa atagttaaag ctattttata aggagatata
     1561 tgatggaagt atatgctttg gttgggtcaa gtggcacagg gaagagcttt aaggctttag
     1621 agttcgcata tgaaaatgat atagaatata taattgatga tgggatttta atatacaaaa
     1681 acaaggtttt agctgggata tcggcaaaac aagcaaatac tactatagaa gcagttaaaa
     1741 gggctatttt ttataatctg gagcatagac aagaagttag agaagcaatt aaaaaagaaa
     1801 atataagaaa aattcttatt attgggacat caaaaaaaat ggttaatcaa attgtagaga
     1861 gattgtcaat tggaagggtt tgcaagttta taaatataca agatataagt actaaatatg
     1921 aaatagaaat agcaaaacaa gctagaaaag aaggaaatca tgtaattcct gttccagcag
     1981 ttgagataaa aagtatggca agtggtctta gtataaattc tttgaagaga ctttttagaa
     2041 aaggaaataa taggaatatg gtagttttag aaaaaacaat tataagacca acttttagtt
     2101 atataggaaa gttttatatt agtgctgatg taattaaaca gattattgaa tatgaagtat
     2161 acaactttgg aagcattgat agaataaata aaatcaatat agagaattgc aataatttta
     2221 tgaatatttt tgtaagtata aatatcaatg atttagaaac tataaaaaaa attgaagaca
     2281 tacaacagac aataaaaaag tctattgaga aaatgacttt agttaatgta caaaaaatag
     2341 atattaacat tcataaaatt aaaaaaataa acttcctcca atgttaaaaa ataaactgag
     2401 aaaatgatat actaatttgc aataaagtgt gctataatta aactgtaaat ttaggaaatg
     2461 gttttttcta aatggagtat acataattat aacgtattta taagggggct ttcaaatgtc
     2521 aggaaaagat gtaaatgtct tcgagatggc gcaatctcaa gtaaaaaatg catgtgataa
     2581 attaggtatg gaaccagcag tttatgaatt attaaaagaa cctatgagag ttatagaggt
     2641 ttcaattcca gtaaaaatgg atgatggttc tataaaaact tttaaaggat ttagatcaca
     2701 acataatgat gcagtagggc caacaaaagg tggaataaga ttccatcaaa atgtttcaag
     2761 agacgaagta aaagctttat ctatatggat gactttcaag tgttctgtaa caggtatacc
     2821 atatggtgga ggtaaaggtg gaataatagt agatccatca actttatctc aaggtgaatt
     2881 agaaagatta agtagaggat acatagatgg aatatataaa ttaataggtg agaaagttga
     2941 cgttcctgca ccagacgtaa atactaatgg tcaaataatg tcttggatgg ttgatgagta
     3001 caataaatta actggacaaa gttctatagg tgttataact ggtaagccag ttgaatttgg
     3061 tggttcttta ggaagaacag ctgctactgg atttggtgtt gctgttactg caagagaagc
     3121 tgctgctaaa ttaggaatag atatgaaaaa agctaaaata gctgttcaag gtataggaaa
     3181 cgttggttct tatacagttc ttaactgtga aaaacttggt ggtactgttg tagctatggc
     3241 tgaatggtgt aaatcagaag gttcttatgc tatatacaat gaaaatggtt tagatggtca
     3301 agctatgtta gattatatga aagaacatgg taacttatta aacttcccag gagctaagag
     3361 aatatcttta gaagagttct gggcttcaga tgttgatata gtaataccag ctgcattaga
     3421 aaactctata actaaagaag ttgctgaatc tataaaagct aaattagttt gtgaggctgc
     3481 taatggacca actactccag aggctgatga agtatttgct gaaagaggaa tagttcttac
     3541 tccagatata ttaactaatg ctggtggagt tacagtttct tacttcgagt gggtacaaaa
     3601 cttatatgga tactactggt cagaagaaga agtagagcaa aaagaagaaa tagctatggt
     3661 taaagcattt gaatctattt ggaaaatcaa agaagagtac aatgttacaa tgagagaagc
     3721 tgcttacatg cattcaatta agaaagttgc tgaagctatg aaattaagag gatggtacta
     3781 agatttactc ttaataaata ataggatata attatttaag atataaaggg gctgtacata
     3841 gtacagccct taaattatgt ggcttacaat gaagtaagct atttttgttt taacttataa
     3901 ataacttgat aaattatgta atttagaatg tattttttac caccacatga ttacaaatta
     3961 gaggagttag attgaatttt tagacatgaa cttatgcatt ttaaaagcaa agaccttaat
     4021 ttaaagtatc tagtattatt tttaaaaata gtatattggt ttaatccatt tatatgtata
     4081 atggaaaaat atatggattt ggattgtgaa ttttattgtg atgagagagt tttaaaaaat
     4141 tgtactgtac aacaaaagca agaatatgct ctaactattg taaattcaat gaaaagaggt
     4201 acaaattttt caaataaatt tgtagctgga ttaaataaac aatctgatat tataaaaaga
     4261 gtaatgaaca tgttcagtga aaaacataaa aatggtatct taatggcttt gattataagc
     4321 ttgttttctt ctattacatt ttttaaattc gattcaacta gtattattga agagaatgat
     4381 ttcttagaga ggttaagata tacgtttgaa ggaaggcaag aagatatgtt tgtaacaata
     4441 gattttacat atgcagatac acctataaaa cttagaaaag agcatgaaga aaactgtaga
     4501 gtcaatggaa tagtaccaaa agattcagat tcaatagaaa ttattcctag attttataaa
     4561 gattgtatgg atttaaaaaa ataagttaga cttaatattg atgttgatat tatgaatttt
     4621 ttatatttaa gcaattgaaa attttattat agattcaatt ttatatgtgt ttaagtatga
     4681 aattt
//
LOCUS       DAELLV010000256         4660 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.256, whole genome shotgun sequence.
ACCESSION   DAELLV010000256 DAELLV010000000
VERSION     DAELLV010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4660
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.256"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(63..1121)
                     /locus_tag="KPD58_003257"
     CDS             complement(63..1121)
                     /locus_tag="KPD58_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBG0266987.1"
                     /translation="MNKKKITFIFVILILALILFSFFRSSSQNTKYSKTSYYLGTVNE
                     VTVFNVKESKSDKILNECDSILRYIDNKMSTHIPGSDVSKINDNAGKKFVRVSDDTFF
                     VVKEAIQYSKLSDGYFDITIGPLSNLWAIGTDKAKVPSDSKIQKLLPLIDYKNIILDE
                     KNKSIKLTKENMKIDLGAIAKGYAADKIVAYLKSEDVKKGLVNLGGNIFTLENGKNDK
                     PFKIGIQDPTSKNGESIGNIETTNKSVVTSGIYERFIEKDGKIYHHMLNPFTGYPFEN
                     NLSSVTIISDKSINCDALSTSTFGLGLEKGMNLINKIDNVDAIFITKDKKVYLTKGIK
                     DNFKLTDKSFSIEKLNKQ"
     gene            1188..1559
                     /locus_tag="KPD58_003258"
     CDS             1188..1559
                     /locus_tag="KPD58_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBG0266988.1"
                     /translation="MKKKDILLILGILVVITACYGIINVINSKNAGNIEIYVDNKLYK
                     TVSINAKEEFKIESRGGYNIVKIHDKGVEIVDASCPDKVCVHTGFINKPSQSIVCIPN
                     RVSIKINTNEKNDNQEDIISK"
     gene            1596..2270
                     /locus_tag="KPD58_003259"
     CDS             1596..2270
                     /locus_tag="KPD58_003259"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBG0266989.1"
                     /translation="MVNLGNDWDELLKEEFEKDYYLNLRKFLIEEYKTRQIFPNMHNI
                     YEALKHTSYKDTKVLILGQDPYHGDNQAHGLAFSVQPQVKTPPSLLNMYKELKDDLGC
                     FIPNNGYLMPWADQGVLLLNTALTVRAHEANSHKNKGWEIFTDRVISILSEREDPVIF
                     VLWGSNARKKVELIDTSKHYILEAPHPSPLSASKGFFGCKHFSKINEILKKLGKEPIN
                     WQIENI"
     gene            2519..3358
                     /locus_tag="KPD58_003260"
     CDS             2519..3358
                     /locus_tag="KPD58_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HBG0266990.1"
                     /translation="MNFKKLSNAKVSVMAVVLSVGILSGYSALNKQVTLVVRGEERTI
                     STFSSNVGDLLESQNISYDSNDIVTLDENTKLSNGDKIEVIDVKEKTVVETKDIPFEV
                     TVVEDGSLLKGDSKVKEEGKSGKSELIYKETYHNGKKVEKEFVKKVVKAEPVNKVVSK
                     GTKVEMQVASSRGESSRRMASSSSSSSSSNNGKNMRVVATAYTGHSITATGTKPKWGT
                     IAVDPKVIPYGTKVYIPQFDMVFTAEDCGGAIKGNKIDIYMNDSKTVYNWGRKTIDIR
                     ILK"
     gene            3602..3698
                     /locus_tag="KPD58_003261"
     tRNA            3602..3698
                     /locus_tag="KPD58_003261"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3636..3638,aa:Sec,seq:tca)
     gene            3849..4556
                     /locus_tag="KPD58_003262"
     CDS             3849..4556
                     /locus_tag="KPD58_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HBG0266991.1"
                     /translation="MENNFTTRTSFLVGDDGIKKLNNSNIIVFGVGGVGSFTVEALAR
                     AGVGNITIVDFDDVDITNINRQIPALHSTVGRYKVDVMEERILDINPNINIKKIRSLY
                     NKDTSDEILTERYDYVVDAIDMVSSKIHLIETCEKKGLKIISSMGMGNKLDPTKIVVT
                     DIHKTSTCPLAKVMRKELRDRGIKKLKVVYSTEQPIELKKKVMNGRKVTPGSVSFVPS
                     VGGLIIASVIVNELLGQ"
BASE COUNT         1812 a          539 c          751 g         1558 t
ORIGIN      
        1 gtgataacta tttaaattca tatgtatttt gtatgtataa tagatataat tattatttaa
       61 acttattgtt tatttaattt ttcaatagaa aaagatttat ctgtaagttt aaaattatct
      121 tttataccct tagttaagta tactttctta tccttagtta tgaatatagc atctacatta
      181 tcaattttat taattaaatt catacctttt tcaagaccaa gaccaaatgt agaagtggat
      241 aaggcatcac aattaataga tttatcagag attattgtaa cactacttaa attgttttca
      301 aaaggatacc ctgtaaatgg atttaacata tgatgatata tttttccgtc tttttcgata
      361 aatctttcat aaattccaga agtaacaaca gacttatttg tagtttctat gtttcctatg
      421 gattcaccat ttttactagt tgggtcttga atacctattt taaaaggttt atcattttta
      481 ccattttcta aagtaaatat atttcctcct aagtttacta aacctttttt tacatcctct
      541 gattttaaat aggctacaat tttatcagca gcatatcctt tagctatggc acctaaatca
      601 attttcatat tttctttggt taattttata gatttatttt tttcatccaa tatgatgttc
      661 ttataatcaa taagaggcag aagtttttgt atcttagaat cacttggaac tttagcttta
      721 tctgttccaa tagcccataa atttgataat ggaccaattg ttatgtcaaa atatccatct
      781 gataatttgg aatattgtat agcttctttt acgacgaaaa atgtatcatc agaaactctg
      841 acaaattttt taccagcatt atcatttatt ttagaaacat cacttcctgg tatatgagtg
      901 ctcattttgt tgtcaatata tcttaaaatt gaatcacatt catttaagat tttatcactt
      961 ttggattctt ttacattaaa tacagtaact tcgtttactg taccaaggta gtagctagtt
     1021 ttactatatt tagtattctg gcttgaactc ctaaaaaatg aaaataaaat taatgctaaa
     1081 atcaatatca caaaaataaa tgtgattttt ttcttattca tggtatattc ctcctatgaa
     1141 attgtgttat tattttatta taacataatt tcataggagg acaaaaaatg aagaaaaaag
     1201 acattcttct tattttaggc attttggttg ttattacagc atgttatggg ataataaatg
     1261 ttataaattc taaaaatgct ggaaatattg agatttatgt agataataaa ttgtataaaa
     1321 ctgtttcaat aaatgccaaa gaagaattta aaatagagag tagagggggg tataacatag
     1381 taaagattca tgataaaggg gttgagatag tagatgcttc atgccctgat aaggtttgcg
     1441 tacatactgg atttataaac aagccaagtc aaagcatagt ttgtatacct aatagagtga
     1501 gcataaaaat aaatacaaat gaaaagaatg ataatcaaga agatataatt tctaaataaa
     1561 ataataaaaa taattgaaag gaattggtga taattatggt taacttagga aacgattggg
     1621 atgaattatt gaaagaagaa tttgaaaaag attactactt aaacttaaga aaatttctta
     1681 tagaagaata taaaacacgc caaattttcc caaatatgca caatatatat gaagctttaa
     1741 aacacacttc atacaaagac acaaaagtct taatattagg acaagaccct tatcatggag
     1801 ataatcaagc tcatggttta gctttttcag ttcaaccaca agtgaaaact ccaccatctt
     1861 tattaaatat gtataaagaa ttaaaagatg atttaggttg ttttatacct aataatggat
     1921 atttaatgcc ttgggcagac caaggagtgt tacttttaaa tactgctctt actgttagag
     1981 cgcatgaagc taattctcat aagaacaaag gatgggaaat tttcactgat agagtaatat
     2041 ctattttaag tgagcgtgaa gacccagtta tttttgtact atggggaagt aatgcacgta
     2101 aaaaagtaga gttgattgat acaagtaaac attatatttt ggaagcacct catccaagcc
     2161 cattgtcagc aagtaaaggc ttttttggat gtaaacactt ctctaagata aatgaaattt
     2221 tgaaaaagct aggaaaagaa cctattaatt ggcagataga aaatatataa aagtaaaagg
     2281 taacaaaatg ataacaaaac aacaataaac aatgacattg ttgtaactaa tagaaatatg
     2341 cttaaatagt tgaaaatact atgtatgcat atttctattt ttttgtcaag ttgacaactc
     2401 ttgaaaaaat agttacaatt tagttacaca aaaaacacaa ataaaaaaag aaacttacaa
     2461 agcagaattt tatgaagcaa aatatatagt gtaaaaaatc ttagggagga aattttatat
     2521 gaattttaaa aaattgagta atgctaaagt ctcggtaatg gcagttgttt tatcagttgg
     2581 aatattatcg ggatactctg cgcttaataa acaagtaaca ttagtagtaa ggggagaaga
     2641 aagaactata tcaacattta gttctaatgt aggagacctt ttagaatctc aaaacattag
     2701 ctatgattca aatgatatag taactttaga tgaaaataca aagttatcta atggagataa
     2761 aatagaagta attgatgtaa aggaaaaaac agttgtagaa actaaagaca ttccatttga
     2821 agttacagtt gttgaagatg gctctttatt aaagggtgat tctaaggtta aagaagaagg
     2881 aaagtctggt aaaagtgaat taatatataa agaaacttat cacaatggaa agaaagtaga
     2941 aaaggaattt gtgaaaaagg ttgttaaagc tgagccagta aataaggttg taagcaaagg
     3001 gacaaaggta gaaatgcaag tagcctcatc aaggggagaa agttcaagaa gaatggcttc
     3061 atcatcatct agtagcagta gtagtaacaa tggtaaaaat atgagagtgg tagcaactgc
     3121 atatactggt cacagtataa ctgcaacagg aacaaaacct aagtggggaa caatagctgt
     3181 agaccctaag gttataccat atggaactaa agtttatata cctcaatttg acatggtgtt
     3241 tactgcagaa gactgtggtg gagcaataaa aggaaataaa attgatatct acatgaatga
     3301 ttcaaaaaca gtttataact ggggaagaaa aactatagat atacgtatat taaaatagtt
     3361 aaataattta tatttttact ggaaattata ttttgattat atagagaaga aactctttat
     3421 agattctaaa aatataattt ccagtttttt attttggata tgtaataaaa caggtaaatt
     3481 ttaaccatgt atagataaaa gctatttaaa ttataattta ctatcatata aatctataaa
     3541 aattgttgca aagagtcaaa atatatgata ttatagattt cgttgagaag gatttcatta
     3601 tgggagtgga tgggtgccgg tgtgccctct agtcttcaaa actagtatga ggggttaaga
     3661 gcctcttagg tgggttcgat tcccacgcat tcccgccaat atgtaaaagg tatcttttaa
     3721 ttaaaagata cctttttaaa tttattttaa gataatttat ttttaataat atataatgtt
     3781 ttgtattata taatgtaaat tttctaatac tattatatat ttagataaaa atctaggagt
     3841 gtggaaagat ggaaaataat tttacaacga gaactagttt tctagttgga gatgatggga
     3901 taaaaaaact taataattca aatataatag tatttggagt tggaggagtt ggtagtttta
     3961 ctgttgaagc tttagcaaga gctggagtag gcaatattac tattgtagat tttgatgatg
     4021 tagatataac caatataaat agacaaatac cagccctaca ttcaactgta ggaagataca
     4081 aagttgatgt catggaagaa agaatattag atataaatcc taatatcaat ataaaaaaaa
     4141 taagaagtct ttataataaa gatactagtg acgaaatact aactgaaaga tatgattatg
     4201 ttgtggatgc gatagacatg gtaagttcca aaatacatct aattgaaact tgtgaaaaaa
     4261 aaggattgaa aataataagt tctatgggta tgggaaataa attagaccca actaaaatag
     4321 tcgttacaga tatacacaaa acaagtactt gtcctctagc taaagttatg aggaaagaac
     4381 ttagggatag aggtatcaaa aagttaaaag tagtatattc tactgaacaa ccaatagaac
     4441 ttaagaagaa agtaatgaat ggtagaaaag taacacctgg tagtgtatca tttgtcccat
     4501 ctgtaggagg tttaataatc gcatctgtaa tagtaaatga actattagga caataagttt
     4561 taaatatttt aatttataaa ttttatataa aaatttgaag gctagaagca tgaatacaaa
     4621 gcaattttat attcatactt ttagcctaat tattttatat
//
LOCUS       DAELLV010000257         4653 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.257, whole genome shotgun sequence.
ACCESSION   DAELLV010000257 DAELLV010000000
VERSION     DAELLV010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.257"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..886)
                     /locus_tag="KPD58_003263"
     CDS             complement(<1..886)
                     /locus_tag="KPD58_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG0266992.1"
                     /translation="MYSTKILDDYEYETFNVFSTKLNPNTKHDYLSKVILFKEFLKGK
                     ELIYATKEDCKNFVDYIQTKYAKSTCEKIYSYLHSFYNFLKKEGYIDINPFRYVEKPT
                     VTRIKTKDDVLSIQEINKLIGILPKLNIRDRVIIVCLVTTGCLLNELVSLKWKDLMVD
                     ENDNSYVRLGKGRKERVVKLHPYFFKLLEDYRNYSGLPEVIIPSDDFIFTTQKSNSIT
                     DRNVRLIVKKALDLAGLSQYSARDFRHSFAAISLRLGADESDVKNQLGWSDKYYAIRY
                     KYVLNFVDSEIVDYLIEKD"
     gene            1143..1967
                     /locus_tag="KPD58_003264"
     CDS             1143..1967
                     /locus_tag="KPD58_003264"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBG0266993.1"
                     /translation="METFSLLEIFKAVILGIVQGITEWLPVSSTGHMILVDEFIKLNF
                     SNTFISTFLVVIQFGSILAVLVIFFRKLNPFDSAKNIKQKKETVRLWLKVIIAVIPSG
                     VIGILFEDDIDRLFFNSTVVAIALIVYGIIMIGLEKRNKKPKYKDFSQVTYKLALCIG
                     LFQCLALIPGTSRSGSTIIGAVLLGTSRYVAAEFSFFLAIPTMLGASALKLLKAGFGF
                     TGFEWLILGVGSVVAFVVSIVVIKFFMDYIKKHDFKVFGYYRIVLGIVVLAYFFLL"
     gene            2007..2537
                     /gene="lepB"
                     /locus_tag="KPD58_003265"
     CDS             2007..2537
                     /gene="lepB"
                     /locus_tag="KPD58_003265"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBG0266994.1"
                     /translation="MSVKKEIFDWIKSIAMAIVLAFVILQFIRPSIVSGESMYPTLDD
                     KDYLILNRISYKVGKPEKGDIVVFKTNLVDGETGKKKDLIKRVIATEGDRIKISNSKV
                     YVNGKLLNEPYIHNNYTSGDIDTVVPKGKLFAMGDNRENSNDSRFPDVGMVDEDEVLG
                     KVMVRLLPLDNIGKVD"
     gene            complement(2619..4400)
                     /locus_tag="KPD58_003266"
     CDS             complement(2619..4400)
                     /locus_tag="KPD58_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HBG0266995.1"
                     /translation="MSQYININSQLDALKLKEFEKIFYENINEDVKVVPKVTPFKGIN
                     TDLLYVKDGRILFIKFMDTTEDIFFILEEELLEVMNEEYELLKLKMGQKNRNISYNYV
                     YIMPYVEVEETYEFEEFVNNNIIDKNKLQDIMNKGSLEEYLNDENDEINLNLFLLDIC
                     SEYYIINDKLHLNEKFKKISFYNDDYKYTATMMEDVQIKDVVSIKYGNTLIEGGSGIG
                     KTAIMLSRAIKLAKVYPHHKLVIFTHTKQLCNELRERIELLYKDNNNLEVHTFSSFIF
                     KLAKKFNLIVDYNMLKNDYEKTFNNLVKQAQNIIKNKNMFKAIFIDEAESFLEYEIDF
                     IREFLYKTKFIFNVCSCNSLNISNRLNIFKKLYNGIEFDDKIILSKNYRQAKEIVDFT
                     NKFSNNSNSYINELRPNTEFSTFFYTKALRGGNKSVDIIKVSDLDDQISSVIWEIEYL
                     ISKKGLDYSEVAIVYPYNKKKLKSGKTIYFQYMLKKALEEAKIPYICAEDNLTNISKK
                     VGTTIANIYAIKNLEYKAVIVCELEMLYNQTINDIEQDYQVNDFVGDLNKVYLAMSRA
                     TDYLSIVTTFNEEASDIIRLITESKDI"
BASE COUNT         1570 a          644 c          574 g         1865 t
ORIGIN      
        1 tatctttttc aattaagtaa tccactattt cactatcaac aaaatttaat acatacttat
       61 atctaattgc atagtattta tcactccatc caagctgatt ttttacatca ctttcatctg
      121 ccccaagcct taaacttatt gctgcaaatg aatgtctaaa atctcttgct gaatattgag
      181 aaagtccagc taaatctaat gcttttttga ctataagtct gacatttcta tctgttatag
      241 aattactctt ttgagttgta aaaataaaat catcactagg tattattacc tctggaagcc
      301 cagaataatt cctgtaatct tctaaaagtt taaaaaagta tggatgtaat ttaactactc
      361 tttcttttct tcccttacct aaccttacat atgagttatc gttttcatct accataaggt
      421 ctttccactt taagctaact aattcattta ataaacaccc agtagtaact agacagacta
      481 ttattactct atctcttata tttaattttg gtaggatacc tataagttta tttatttctt
      541 gtatacttag aacatcatct tttgttttta ttcttgttac tgtgggtttt tctacatatc
      601 taaatggatt tatatctatg tatccttctt tttttaagaa gttataaaaa ctatgtaaat
      661 aactatatat cttttcacaa gttgatttcg catatttagt ctgtatatag tctacaaaat
      721 ttttacaatc ttctttcgta gcataaatca attctttccc ttttaaaaat tctttaaaca
      781 gtataacctt tgataaataa tcgtgtttcg tgtttggatt taattttgta gaaaaaacat
      841 taaaagtttc atactcataa tcatctagta tttttgtact atacataata actcctttcc
      901 ttttcataaa ataaaatagc tatactttta ctataccata tatttccccc tcaaatatac
      961 cccttattca atagataact tttaagttta ttttgttata ttatgaaggt tttatgaaaa
     1021 tatcaatctt ccattgtgca ctacacagta ctatggtatt ataagttagt aaaaggtggc
     1081 atttttttat taatctatga ataaaatatg ctactataaa tttgtattgg aggtattttg
     1141 atttggaaac atttagtttg cttgaaattt ttaaggcagt cattttgggc atagttcagg
     1201 gaataacaga atggctacca gtaagtagta cagggcatat gatattagtt gatgagttta
     1261 taaaattgaa tttttcaaat acatttataa gtacattttt agttgtaatt caatttggtt
     1321 caatattagc tgtacttgtt atatttttta gaaaattaaa tccttttgat agcgcaaaga
     1381 atattaagca gaaaaaagaa acagtccgtt tatggcttaa ggttataatt gcagttatac
     1441 catctggagt tattggaata ctgtttgaag atgatataga tagattattc tttaattcaa
     1501 cagtagttgc tattgctctt attgtatatg gtataataat gatagggctt gaaaagagaa
     1561 ataaaaaacc taagtataaa gatttttctc aagttacata taaattagct ctttgtatag
     1621 gattattcca gtgtctagct cttataccag gtacatctag gtctggttct actataatag
     1681 gtgcagtatt acttggtact tctagatatg tagcagcaga gttttcattc ttcttagcca
     1741 tccctacaat gcttggagct agtgcattaa aacttctaaa agctggattt ggatttactg
     1801 gatttgaatg gcttatttta ggtgttggtt ctgtagtagc atttgttgtt tcaattgtag
     1861 ttattaaatt ttttatggac tatatcaaaa aacatgactt caaagtattt ggttattata
     1921 gaatagtctt gggtatagtg gttcttgctt atttctttct attataatgt attctaattt
     1981 aataacaaat gataaggaga aaagttatga gtgttaaaaa agaaatattt gattggatta
     2041 agtcaatagc tatggctatt gtacttgcat ttgtaattct acaatttata agaccttcta
     2101 ttgtaagtgg agaatcaatg tatcctactt tagatgataa agattatctg attttaaata
     2161 ggatatcata caaggttggt aaacctgaaa aaggcgatat tgtagttttt aaaaccaatt
     2221 tagttgatgg agaaacagga aagaaaaaag acttaataaa aagagttata gctactgaag
     2281 gtgacagaat aaaaatatca aattctaaag tgtatgtaaa tggaaaatta ttaaatgaac
     2341 catatataca caataactat acttctggag atatagatac tgttgttcca aaaggtaaac
     2401 tatttgcaat gggagataat agagaaaata gtaatgatag tagattccct gatgtaggta
     2461 tggttgatga agatgaagtt cttggtaagg ttatggtgag actattacct cttgataata
     2521 ttgggaaagt agactaaaaa ataagagact gaattggtaa agtttatttt acaaatctca
     2581 gtctcttttt attttcttaa attacaaata tttttttact aaatatcttt tgattctgta
     2641 ataagtctta ttatatcact tgcttcttca ttaaaagttg ttactatact taggtagtct
     2701 gttgctctac tcatagctaa ataaacctta tttaaatctc ctacaaagtc atttacttga
     2761 taatcttgtt caatatcatt tatagtttga ttgtataaca tctctaattc acatactatt
     2821 accgctttat actctaaatt ctttattgca tatatatttg ctattgtagt tcctaccttc
     2881 ttacttatat ttgtcaaatt atcttctgca catatataag gaatttttgc ctcttccaat
     2941 gcttttttta acatatattg aaagtaaatg gtttttccac tttttaattt cttcttattg
     3001 taaggataaa cgatagcaac ttcagaataa tcaagacctt ttttacttat taaatactca
     3061 atttcccaga ttacagaact tatttggtca tcaaggtcac ttacttttat tatatcaaca
     3121 gacttatttc cacctctaag tgctttagta taaaagaaag tactaaattc tgtatttggt
     3181 cttaattcgt tgatatatga gttagaatta tttgaaaatt tatttgtaaa atctacaatc
     3241 tctttagctt gtctataatt tttacttaag ataatcttat catcaaattc tataccatta
     3301 tataattttt taaatatatt caatctatta gagatattca aggagttaca agaacacaca
     3361 ttaaatatga attttgtttt atataaaaac tcccttataa aatctatttc atactctaaa
     3421 aagctttctg cttcatcaat aaaaatagcc ttgaacatat ttttattttt tataatgttc
     3481 tgtgcttgtt ttacaagatt attaaaggtc ttttcgtaat cattttttaa catgttataa
     3541 tctacaatta aattgaattt tttagctagt ttaaatataa aagaactaaa tgtgtgaact
     3601 tctaaattgt tattatcttt gtataataac tctatcctct ctcgaagctc attgcacagt
     3661 tgttttgtat gtgtaaatat gactaattta tgatgtggat atacttttgc taattttata
     3721 gccctagaaa gcataattgc tgttttgcct ataccagaac caccttcaat aagtgtattc
     3781 ccatatttaa tagaaacaac atctttaatt tgaacgtctt ccatcatagt tgctgtatac
     3841 ttatagtcat cattgtagaa tgaaattttt ttaaattttt catttagatg aagtttatca
     3901 tttattatat agtattctga acatatgtct agtaaaaata aatttaagtt tatttcatca
     3961 ttctcatcat taagatattc ctccagcgaa cccttattca ttatatcttg aagcttattt
     4021 ttatctatta tattattatt aacaaattct tcaaattcat atgtttcctc aacttcaacg
     4081 tatggcataa tgtaaacgta gttataactt atatttctat tcttttgtcc catttttagt
     4141 ttcaaaagtt catattcctc attcataact tcaagtaact cttcttctaa aataaaaaat
     4201 atatcttctg ttgtatccat aaattttata aataaaattc ttccatcttt aacatacaac
     4261 aaatcggtat ttattccttt aaaaggagta acttttggca ctactttaac gtcctcattg
     4321 atattttcgt aaaatatttt ctcgaactcc ttcaatttta gggcgtctaa ctgagaattt
     4381 atatttatgt attggctcac ttacattacc tccttctgtt atacttaatt ataccatttt
     4441 gaactatatt tttatatata ctttttatag gcaaatttag taaataaatt caaaataaat
     4501 acaaataaat acaaaactgc taaaaaaatt ttgcaataag tatagatatt tattgttata
     4561 aaatactttc tgtaaataat aaaatgataa atttcataat taatgcttaa aataataagt
     4621 aaagtcttaa aaaaaataaa tcttgttagc caa
//
LOCUS       DAELLV010000258         4620 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.258, whole genome shotgun sequence.
ACCESSION   DAELLV010000258 DAELLV010000000
VERSION     DAELLV010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4620
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.258"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            35..949
                     /locus_tag="KPD58_003267"
     CDS             35..949
                     /locus_tag="KPD58_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG0266996.1"
                     /translation="MDIIEGYIDYIKDKKKLSENTVASYFMDIKKYMEYINQKSIKLV
                     DIVENDIISYLIKLEKDNVSIATIARMISSIKSFHDYLFLNHICTNNPAKDIKKPKIK
                     KENINILTEEEIERLLNFPKLTTPKLIRDKAIFEVLYGTGIKVSELVEMNIEDIDLDI
                     DYIYCNSCCKNQRVIPLCDITKLYLEKYLKEARPKMAVEGEKSLFVSSLGQRFTRQGL
                     WKVIKKYSNLANIDKNINPTMLRHSFAIHLLNEGANIAVVSKILGNVNLSSLQVYLNH
                     IDKNVRREIKEKHPRNDVDVELQKAEAK"
     gene            991..2163
                     /locus_tag="KPD58_003268"
     CDS             991..2163
                     /locus_tag="KPD58_003268"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBG0266997.1"
                     /translation="MSRVIWIVIDSVGIGALPDAEKFGDSKDVSTLGNIFKEYPDIQI
                     PNMRNLGIGNIDGIDFFESIKEPIGCFGKCKEMSQGKDTTTGHWEMTGIIVDKPFKTF
                     EHGFSKEIIEEFEKKTGRKVVGNKPASGTVIIDEYGEHQIKTGDVIVYTSADSVFQIA
                     ANEEVIPLEELYNMCKIAREIMMGDNAVARVIARPFIGKKKGEFVRTSNRRDYSLDPF
                     EPTVLDNIKESGLDVLAVGKIEDIFNGKGITDAIHTKSNMDGVDETLNYMKQDNKGLI
                     YSNLVDFDSKYGHRRDPEGYKKALEEFDSRLPEIMANMREDDILIINADHGNDPTYKG
                     TDHTREYIPVMIYGNKIKKGFNLGVKDTFADIGATVADILNVKLPKHGSSFKEDLF"
     gene            2210..3022
                     /locus_tag="KPD58_003269"
     CDS             2210..3022
                     /locus_tag="KPD58_003269"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBG0266998.1"
                     /translation="MFEKIAQSSKFINSKSDIKPKIGLILGSGLGDLANDIEDPVAIK
                     YSEIPNFPVSTVAGHAGQLVIGKLEGKEVIAMQGRFHYYEGYSQKEATFPVRVMKELG
                     VEILIVTNAAGGVNKEFKAGDLMLIRDHINFSGSNPLVGKNDDRFGARFPDMSDAYSS
                     KYVDVVKNCAKECNIDVKEGVYMFFSGPNYETPAEVRMAQILGADAVGMSTVPEVIVA
                     SHSNIGVIGISCITNMAAGILNQPLSHEEVIETTQKVKSEFMNLVKTTVKNL"
     gene            3226..4551
                     /locus_tag="KPD58_003270"
     CDS             3226..4551
                     /locus_tag="KPD58_003270"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBG0266999.1"
                     /translation="MRMYDIIKKKRDNLELSKKEINYFIENYSKGDIPDYQASALLMS
                     IYLNKMNKQETVHLTEAMMNSGDMINLSEIDGIKVDKHSTGGVGDKTTIALIPLVASC
                     GAPVAKMSGRGLGHTGGTIDKLESIPGFSTEMEISKFIDSVNKSKIAVCGQSAKVAIA
                     DKKIYALRDVTATVDNISLISSSVMSKKLASGADAIVLDVKTGDGAFMKTLEESFELA
                     KSMVDIGTGMNRDTIGIVTDMDEPLGFAVGNSLEVIEAIETLKGNGPKDLVELCEVLG
                     AYMLVLSKVAHDFEDGVEKIRESIKSGTAIQKLKEFIENQGGNKDVVDDYSLFKKSKY
                     KYPVKSTKSGYISKIKAEDVGVSAMILGAGRETKDDILDLSAGIILEKKVGDYVNEGE
                     VLAYMYYNDEQKLSLAKGRFIDSYSIVDSKIEKNKLIYGIVTKEEIKKF"
BASE COUNT         1839 a          474 c          897 g         1410 t
ORIGIN      
        1 ttattaagtt cctagctaaa taaaggagtt tataatggat attatagagg gatatataga
       61 ttacataaaa gataaaaaaa aattatcaga gaatacagtg gcatcatact ttatggatat
      121 aaaaaaatat atggaatata taaatcaaaa aagcattaaa ctagtagata tagtagaaaa
      181 tgatattata agttacttaa taaagttgga aaaagataat gtatcaatag ctacaatagc
      241 gaggatgata tcctctataa aatcttttca tgattattta tttttaaatc atatatgtac
      301 aaataatcct gcaaaggata taaaaaaacc taaaataaaa aaagaaaata taaatatact
      361 tacagaagaa gaaattgaaa gactattaaa ttttccaaag ttaacaacac ctaaattgat
      421 tagggacaag gctatttttg aagtattata tggtactggt ataaaagtat ctgaattagt
      481 agagatgaat atagaggata ttgacctaga tattgattat atatattgta attcatgttg
      541 taaaaatcaa agagtgatac cactttgtga tattacaaaa ctttacttag aaaagtatct
      601 aaaagaagca agacctaaaa tggctgtaga aggtgaaaaa tcattattcg taagttcatt
      661 aggtcaaagg tttacacgac aaggtctttg gaaagtcata aaaaaatatt ctaatctagc
      721 caatatagat aaaaatataa atcctacaat gcttagacat tcatttgcta ttcatttgtt
      781 aaatgaaggt gctaacattg ctgttgtaag taaaatttta ggaaatgtta atttatctag
      841 tttgcaagta tatttaaatc atatagataa aaacgtgaga agagaaataa aggagaaaca
      901 tcctagaaat gatgttgatg tagaattaca aaaagcagaa gctaaataga ctatatagca
      961 aattttataa tttagaagga gcatatttat atgagcagag taatttggat tgtaattgat
     1021 agtgttggga taggagcatt gcctgatgct gaaaaatttg gtgatagtaa ggatgtaagt
     1081 acccttggaa atatatttaa ggaatatcca gatattcaga ttcctaatat gagaaattta
     1141 ggtataggaa acatagatgg tatagatttt tttgaaagta taaaagaacc aataggttgt
     1201 tttggaaagt gtaaggaaat gtctcaggga aaagatacta ctacaggtca ttgggaaatg
     1261 actggtataa tagttgacaa accatttaag acatttgaac atggattctc taaggagata
     1321 atagaagagt ttgagaaaaa aactggaaga aaagtagtag gaaataaacc agcttcagga
     1381 actgtaataa tagatgagta tggagaacat caaataaaaa ctggagatgt aatagtatat
     1441 acatcagctg atagtgtatt tcagattgct gctaatgaag aagtaattcc cctagaagaa
     1501 ctctataata tgtgcaaaat cgctagggaa ataatgatgg gggataatgc agtagctaga
     1561 gtaattgcta gaccattcat aggtaagaaa aaaggtgagt ttgtgaggac ttcaaataga
     1621 agagattatt cactagaccc atttgaacca acagttcttg ataatataaa agaatcagga
     1681 cttgatgttt tagcagttgg aaaaatagaa gatattttta atggaaaagg aattacagat
     1741 gctatccaca ctaaaagcaa tatggatgga gtggatgaaa ctttaaatta tatgaaacaa
     1801 gataataaag gtctaatata ttcaaatctc gttgattttg attctaaata tggtcatagg
     1861 agagacccag aaggatataa gaaagctctt gaagagtttg atagtagact tcctgaaata
     1921 atggctaata tgagagaaga tgatatttta ataatcaatg cagaccatgg aaatgaccca
     1981 acatataaag gtacagacca tacaagagaa tatatacctg ttatgattta tggcaataag
     2041 attaaaaaag gttttaattt aggagtaaaa gatacttttg ctgatattgg agcaactgtt
     2101 gcagatatac taaatgtaaa gttgcctaaa catgggtcaa gctttaaaga agatttattt
     2161 taaactataa aatgataaaa acttagctta ataggatgga gagattggaa tgtttgaaaa
     2221 gatagcacaa agtagtaaat ttataaattc taaaagtgat ataaaaccta agattggttt
     2281 gatattaggt tctggactag gtgatttagc aaatgatata gaagaccctg tcgcaattaa
     2341 atatagtgaa attccaaact tccctgtatc aacagtagca ggacatgctg gacaacttgt
     2401 aattggaaaa cttgaaggaa aagaagttat tgctatgcaa ggtagatttc attactatga
     2461 aggatattct cagaaagaag ctacttttcc agtaagggtt atgaaagaat taggagtcga
     2521 gattcttata gtaactaacg ctgctggagg cgtaaataaa gaatttaaag caggagattt
     2581 gatgcttata agagaccata taaactttag tggaagtaat ccattagtag gaaaaaatga
     2641 tgacagattt ggtgctagat ttccagatat gtcagatgca tattcttcta aatatgtaga
     2701 tgtagttaaa aactgcgcta aagaatgcaa tatagatgtc aaagaaggcg tttatatgtt
     2761 ttttagtggt ccaaactatg aaacaccagc agaagtgaga atggcacaaa ttttgggagc
     2821 tgatgctgta ggtatgtcta ctgttccaga agtcatagta gcttctcact ctaatatagg
     2881 agttattggt atatcttgta taacaaatat ggctgctggc atcttaaatc aaccactaag
     2941 tcatgaggaa gtaatagaaa ctactcaaaa agttaaatca gaatttatga atttagttaa
     3001 aacaacggtt aaaaatttat aagttaaagc ttattataga ttttttgagg aagtttataa
     3061 taaaatagtt aatgtttaat ttgaaaagaa ttaataaaaa gctgtcttga tatagaaagt
     3121 tatttctaat aaaggacagt ttttttataa tactacaaca tttgaaagtg ttttaaagtt
     3181 taatatttat ttaaattatt ataggttaga atggagattt caattatgag aatgtatgat
     3241 attataaaaa aaaagagaga taacctagag cttagtaaaa aagagataaa ctactttata
     3301 gaaaactatt caaagggaga tatacctgac tatcaagctt ctgcactatt aatgtcgatt
     3361 tatttaaata agatgaataa acaggaaact gtacatctta cagaagcaat gatgaactct
     3421 ggagacatga taaacctatc tgagatagat ggaataaagg tagataagca tagtactggt
     3481 ggtgttggag ataagactac catagctctt atacctttgg ttgcttcatg tggagcacca
     3541 gttgcaaaga tgtcaggtag aggtcttggt catactggtg gtactattga taaattggag
     3601 tctataccag gtttttcgac agaaatggag ataagtaaat ttattgattc tgtaaataag
     3661 tctaaaatag cagtttgtgg tcaaagtgca aaggtagcaa ttgcagataa gaaaatatat
     3721 gctcttagag atgttacagc aactgttgat aatatatcat tgatatcatc aagtgtaatg
     3781 tctaagaagt tagcttcagg agctgatgca attgtgctag atgtaaaaac tggtgatgga
     3841 gcatttatga agacattaga agagtctttt gagttagcta aatctatggt tgatataggt
     3901 actggtatga atagagatac aatagggatt gtaacagata tggatgaacc tttaggtttt
     3961 gcagtgggaa actctttaga agttatagaa gcaattgaga cattaaaagg aaatggacct
     4021 aaagaccttg ttgagctatg cgaagtatta ggagcatata tgttggtttt atcaaaggta
     4081 gcacatgatt ttgaagatgg tgttgaaaaa ataagagaat caataaaatc tggtactgct
     4141 atacaaaaac tcaaagagtt tatagagaat caaggtggta ataaagatgt agtagatgat
     4201 tattctttat tcaaaaaatc aaagtataaa tatccagtta aatcaactaa atcaggttat
     4261 ataagcaaaa ttaaagctga agatgttggt gtatcggcta tgattcttgg agcaggtaga
     4321 gaaactaaag atgatatttt agatttatca gcagggataa ttttagaaaa gaaagtagga
     4381 gattatgtaa atgaaggtga agttttagct tacatgtatt ataatgatga acaaaagctt
     4441 tcattggcaa agggaagatt tattgattcc tactctattg tagattcaaa aatagaaaaa
     4501 aataaattaa tatatggtat tgtaactaaa gaagagatta aaaagtttta gaaaatttta
     4561 gaaaagtgat tataaaaggc ctaacatgtc aaaaataatg ttaggttttt tattttttga
//
LOCUS       DAELLV010000259         4611 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.259, whole genome shotgun sequence.
ACCESSION   DAELLV010000259 DAELLV010000000
VERSION     DAELLV010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.259"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..621)
                     /locus_tag="KPD58_003271"
     CDS             complement(<1..621)
                     /locus_tag="KPD58_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBG0267000.1"
                     /translation="MKKAINKQFILSTLICFIPFIVSIYFYNRLPNEVAIHFDNYGNP
                     DNYAPKVIAAFGVPLLMLCIHLYTWFRLENEPKKANFSNVIQNLSKWGVAILSVIIQI
                     MMVLYSTGVSLESNFYTGLILGIIVILFGNYLPKCKRNYSVGIILPWTLNDEDNWNKT
                     HHLAGWIWLIGGILLIINAFINIPFYNIFVIFVIVILPFIYSYLMYK"
     gene            complement(602..895)
                     /locus_tag="KPD58_003272"
     CDS             complement(602..895)
                     /locus_tag="KPD58_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG0267001.1"
                     /translation="MGFPETFKALSDPIRREILVMLRENKMSAGEISEKFNMTNATIS
                     YHLSQLKKAGLIFETKYKNFIFYEINISVFEEVMLWFSQFMEKGEKDNEKSDK"
     gene            1288..1338
                     /locus_tag="KPD58_003273"
     CDS             1288..1338
                     /locus_tag="KPD58_003273"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0267002.1"
                     /translation="MTEYIYYYNNKRIKKN"
     gene            complement(1864..2616)
                     /locus_tag="KPD58_003274"
     CDS             complement(1864..2616)
                     /locus_tag="KPD58_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HBG0267003.1"
                     /translation="MFSVIFLCIGGFLAAFVDSIAGGGGLISMPVLMAIGVPVHLAIG
                     TNKFAASAGCISSAYRYAKSGKINNDLLKKLVPFTIIGSVLGVRCVLSISEEILNVLV
                     VVMILIVAIYTFISKNLGQEDNFEAVNKKNLRLGMLMALIMGFYDGFFGPGTGTFLTF
                     GFIKIYGYDFLHASANTKILNLTSNITSLLLFMINGQVDYKIAIVFALVMIMGAYVGA
                     KVAIKKGSKMIKPIFLVMALFMVVKLVYQTLV"
     gene            complement(2675..4330)
                     /locus_tag="KPD58_003275"
     CDS             complement(2675..4330)
                     /locus_tag="KPD58_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0267004.1"
                     /translation="MVDFSESNNEVQKFDWGEVMWIHEPSKTQFNRLSAGIVRFFPGN
                     CQEKHFHLSEEQLLYVIQGEGIQIIDGKKVNIKETSIVYCPPYSEHEIINTGKIDLVI
                     LITYVPHKFSSLRQIPIVFSENNIQELVNVNIIENLANQISNILKLRISVYDLKYKEI
                     FETKKENKFCDICKNIKQCTKKLVKSINNDNFEKVYHCEYGVSSLEIPIVLEENIVGY
                     IECGNFIVYKSIDIDKNLSELSSSSGIDIKYINKIYNEFPLNPKSRLYVLKENLLMMS
                     EFIQEIAKRNFFENQLSIKDEEILKSRKENIRLEEALKKANSRLLEEEYIINPIDMFN
                     NSSKEYPFELELYIEKEIKNMNLEGVYNLIDTNKLRYNDVRDTIQEMIFVLSRTVLRD
                     LEDLKLISYLRNKYNKKISLVNSDEDLWNVLFEFSKECIDKNRAFWRKDKGNLIENIN
                     EYIKKYYKENINLNSISDVFFISPNYLSSIFNERNKVSITEYINLLRIEESKKYLLDR
                     SMSISDICKKVGFNNSSYFSQIFKKFNSITPNEYRKNMLDNKY"
BASE COUNT         1653 a          734 c          403 g         1821 t
ORIGIN      
        1 tttatacatc aaataagaat atataaatgg tagtattaca attacaaaga ttacaaagat
       61 attataaaaa ggaatattaa taaatgcatt aataattaat aatattccac caataagcca
      121 aatccatcca gctaaatggt gtgtcttatt ccaattatct tcatcattaa gagtccatgg
      181 caaaattatt cctactgagt aatttcgttt acatttagga agatagtttc caaataaaat
      241 aactattatt cctaaaatta atcctgtata aaaattagac tccaaactaa ctcctgttga
      301 atataaaacc atcattattt gtataattac tgaaagtata gcaactcccc attttgataa
      361 attttgtata acatttgaaa aattagcttt cttaggctca ttctcaagtc taaaccatgt
      421 atacaaatgg atacatagca ttaacaatgg tacaccaaaa gctgctataa cctttggagc
      481 ataattatct ggatttccat aattatcaaa atgaattgca acttcatttg gtaatctatt
      541 ataaaaatat attgatacaa taaatggtat aaaacatatt aaagtagata aaataaattg
      601 tttatttatc gcttttttca ttatcttttt ctcccttctc cataaattgt gaaaaccaaa
      661 gcataacctc ttcgaaaact gaaatattta tttcgtagaa aataaagttc ttatactttg
      721 tttcaaatat gagtcctgcc ttttttagtt gagatagatg ataagaaatt gtagcattcg
      781 tcatattaaa tttttcacta atttctcctg ctgacatttt attctccctc aacataacca
      841 atatttctct tcttataggg tctgaaagag ctttgaatgt ttctggaaac cccaacacca
      901 tcacctcgct atttagaatt tcatctaaat agaatataac ataacatcca ttttatttca
      961 atagctattt agaaattttt ctaaatagct atttctaaat agttattgaa aattttcata
     1021 ttaagattct caatttactt aagtcgcttt tatttaatct tactttatct aattttttta
     1081 acttaacaaa agtgtattgc accataattg ttaaagcaaa aacctaatga ctgagatact
     1141 tatatatata acaatacttt taattttaaa ttaaaagtag ctaaacccaa tttaaaaagt
     1201 agttaagtcc tatttaaata gaaaagaatg aaacgagaaa tatactgtat aaaagtacat
     1261 aaaaacatct actaattaga tacaactata acagaatata tttattatta taataacaaa
     1321 agaataaaaa aaaattaatt tagcataacc ttgttaaata tagatttgaa tatgaattta
     1381 aagttatgta aaattaaaaa tacaggaatg aaaattccat tcctgtataa aatctaactt
     1441 ttgagtctca gtacaatctt attttgagac aatctttttt gttttatatt ttcttatatt
     1501 taaaatataa cgcatatctt atgcaacata ttcatatttt tctttatatt ctttcatcgc
     1561 atcattccaa gcgtcttgta ctgttacacc tcttttatta gcaatttttt gtgccagttc
     1621 aattatcttc atagctcttc ttgatatgtt cataaacatt cctcctacaa attaccatat
     1681 atttcatata tataaatcaa ggttatattt ttatgatata taatccttct attgtaagat
     1741 tacagtttat atgtgtatta tatatgaact atattagaag ataattagaa tttttataaa
     1801 gctatatgat tagattaaat ataaaagctt tacaattact tataaattta aaaagtctat
     1861 aagctaaact aatgtttgat ataccaattt tacgaccata aataatgcca ttactaaaaa
     1921 tattggtttt atcatctttg aacctttctt tatagcaact ttagcaccta cataagctcc
     1981 cattatcatc actaatgcaa acactatagc aattttataa tctacttgac catttatcat
     2041 gaataataac aatgaagtta tattacttgt taagtttaat atttttgtat ttgcagaagc
     2101 atgtaaaaaa tcatatccat atatttttat aaatccaaag gtcaaaaaag ttcctgtacc
     2161 tggaccaaaa aaaccatcat aaaaacccat tataagagcc ataagcatcc ctagtcttaa
     2221 atttttctta ttaacagctt caaaattatc ttcttgacct aaatttttag aaataaaggt
     2281 gtaaatagcc actattaaaa tcataaccac aaccaaaaca tttaatatct cttcacttat
     2341 tgataataca catctcacac ctagaacact accaataata gtaaatggta ctaatttttt
     2401 taaaaggtcg ttattaattt ttccagactt tgcatatcta taggcactac ttatacatcc
     2461 tgctgatgct gcaaatttat ttgtaccaat ggcaagatgt actggcactc caattgccat
     2521 aagaacaggc atgcttataa gtcctcctcc tcctgctata gaatctacaa aagcagctaa
     2581 aaacccccct atacataaaa aaataactga aaacataaaa ttcctccatc attctctttc
     2641 aatttttctt acaatatata ttatataatc ttatttaata cttattatcc agcatattct
     2701 ttctatattc atttggagtt atactattaa acttcttaaa tatttgtgaa aaataactac
     2761 tgttattaaa acctactttc ttacaaatat cactaatact catgcttcta tctaacagat
     2821 attttttaga ttcttctatc ctaagaagat ttatatactc agttattgaa actttatttc
     2881 tttcattaaa aatagaactc aaataatttg gacttataaa aaaaacatct gatatactat
     2941 tcaaatttat attttcttta taatattttt ttatatattc gtttatattt tcaattagat
     3001 ttcctttatc ttttctccaa aatgctctat tcttatcaat acactctttg gaaaattcaa
     3061 ataatacatt ccataaatct tcatcactgt ttacaagtga tattttttta ttatatttat
     3121 ttcttaaata agatataagt tttaaatcct ctaaatctct aagcactgtt ctagacaata
     3181 caaatatcat ttcctgaata gtatctctaa catcattata tcttaatttg tttgtatcta
     3241 ttagattata aacaccttct aaattcatat ttttaatttc tttttcaata taaagttcta
     3301 attcaaaggg atattctttt gaagaattat tgaacatatc tatcggatta ataatatact
     3361 cttcttctaa taatctacta tttgcttttt taagtgcctc ttcaagtctt atattttctt
     3421 ttctactctt caatatctcc tcatctttta tactaagttg attttcaaaa aaattcctct
     3481 tagctatttc ctgaataaat tcagacatca ttaataaatt ttcttttaat acatataacc
     3541 tacttttagg atttaatgga aattcattgt aaattttgtt tatgtatttt atatcaatac
     3601 cagaactact tgataattca gataaatttt tatctatatc aatagactta tatactataa
     3661 aattaccaca ttctatatat ccaactatat tttcttctaa tactataggt atctctaaac
     3721 tggaaacacc atactcacaa tggtacactt tttcaaagtt atcattattt atgcttttaa
     3781 caagtttttt agtacactgt tttatatttt tacaaatatc gcaaaattta ttttctttct
     3841 tagtctcaaa tatttcttta tactttaagt catatacaga tattctaagc ttcaatatat
     3901 tagatatttg atttgcaaga ttttcaatta tgtttacatt aactaactcc tgtatattgt
     3961 tttcagaaaa tactataggt atttgtctca aactcgaaaa tttatgagga acataagtaa
     4021 ttaatattac taaatcaatt ttaccagtgt ttataatttc atgttctgaa tatggtgggc
     4081 aataaactat agatgtttcc tttatattga cctttttacc atcaattatt tgtattcctt
     4141 ctccctgaat cacatataaa agctgttctt cacttaaatg aaaatgcttt tcttggcagt
     4201 ttcccggaaa aaatcttaca attccagcac taagtctatt gaattgtgtt tttgatggtt
     4261 cgtgtatcca cataacctct ccccaatcaa atttttgtac ttcattattt gactcactaa
     4321 aatccaccat aaactcacct cgtagttttt gtctaaatat tttttcttcc cttcttatat
     4381 taaaataata tcatcatcat taactttcat tactataaaa tcatcatact cttttaatac
     4441 tcctgtaatt ttagttgcac ttagtttact acaattcagt tttttaatat attgataaat
     4501 tctataatta cttttttatt ttgtaaaatc agtgttttcc caaaataatt atatctataa
     4561 atgaaagtat ttttactgct tacatcaata tatgttatca taattttcct t
//
LOCUS       DAELLV010000260         4603 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.260, whole genome shotgun sequence.
ACCESSION   DAELLV010000260 DAELLV010000000
VERSION     DAELLV010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.260"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            98..1591
                     /locus_tag="KPD58_003276"
     CDS             98..1591
                     /locus_tag="KPD58_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG0267005.1"
                     /translation="MKYTFEKFKLNEKILKSLKSLGYNIPSRVQREVIPKLLKGQNLV
                     VRSKTGSGKTASFAIPLCENINVDYNNVQALIVVPTRELALQVKDEISDIGRLKKVRC
                     SAIFGKQSIKDQIAELKQRVHIVVATPGRILDHINRGSIKLENVKYLVIDEADKMFNK
                     GFVEQMEKILLNLPKEKIVSLFSATIDEEIKYICEKYMLDYSVINIEENESDTNQKTR
                     QIDDKIIKANGREKYILLKELIYSENPKSVIIFCNTKEKVSKLYNKMSKEGFLIRELH
                     ADLSQERRIFVIKDFKNQKFNILVSSDVASRGIHIDDISLVINYDVPQDKENYIHRIG
                     RTGRKGNSGKAITIVTEKDEKYIENIETYIGYKINELKDIEQSRITHGKVLFEEYSKG
                     VLKEVSKRKKVDKNSYKSKDIKLNIESEVVKLYLNAGKKKKIRVLDIVGAFSNIKGIT
                     NDDIGVIEVQDLCSYVDILNYKGDLILKKYKEIPIKKKMVKVKRDIR"
     gene            1665..1898
                     /locus_tag="KPD58_003277"
     CDS             1665..1898
                     /locus_tag="KPD58_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0267006.1"
                     /translation="MKAEDMKRKVSPIDLERILVTGGVIKHEDSIWLLDFWGNKDIAG
                     ILLMPPTRHVILHLNDCCKWKEYFRTKKKFYRS"
     gene            2054..2818
                     /locus_tag="KPD58_003278"
     CDS             2054..2818
                     /locus_tag="KPD58_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0267007.1"
                     /translation="MAYFNYNDKQCYYNEIGDGTPLLFLHGNTASSKMFNDIIDFYKD
                     EYKVILIDFVGHGKSQMVDKFSADLWFDEAMQVICFLEAMNYKKVNIIGSSGGALVAL
                     NVALERPDLVNKVIADSFEGEVPLESFVQNVKIEREASKQDDGAKAFYIYNQGENWEK
                     VVDNDTEAIFEHYKTIGKFFHKPLETMQPEVLLTGSREDEFVSLISSDFFENTFSLLL
                     EKIKNGKMYLFDKGGHPAILSNGLDFSNVAKKFLEE"
     gene            complement(3103..>4603)
                     /locus_tag="KPD58_003279"
     CDS             complement(3103..>4603)
                     /locus_tag="KPD58_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG0267008.1"
                     /translation="KALYINDVIRSKNLKFIDGKKELDSICEKFRNFDSINLEIPANL
                     NANLRDYQINGLNWFKVLDYYKFGGILADEMGLGKTIQTIAFLLSLSNKKSLIVTPTS
                     LIYNWKNEFEKFAPDIKVLLIHGNKRDREKCFMELENFDVILTTYGTLRNDLEKYSEI
                     KFDYCILDEAQNIKNPVALVTESVKSINAENKFALTGTPMENNLLELWSIFDFIMPGY
                     LYSKAKFQELFINKEDNVKNLKKLIKPFILRRSKKQVMKELPDKIEKNFFVELNKEQK
                     KIYSVYSKDIQDKMKDKNLKKDKIVIFSYLTKLRQLCLDPSIVVKDYNKKSSKIETCL
                     EILRDSINENHKILLFSQFTSVLKNISKELDKYKIKYHYIDGKTNAKERLELVDEFNN
                     SMDKKVFLISLKAGGTGLNLTSADMVIHFDPWWNPSVENQASDRAHRFGQKNSVQVIK
                     LIAKGTIEEKIIKLQESKKELINQFINGELSNEGVLKSLSDEEIINLFN"
BASE COUNT         1774 a          494 c          712 g         1623 t
ORIGIN      
        1 agtctaataa ataatataaa aatagtagaa ccttgtatta taataatata atgtagaaaa
       61 atatatagat aagataaata taataaggtg gtaatatatg aaatatacat ttgaaaaatt
      121 taagttaaat gaaaagatac ttaagtcatt gaagagttta ggatataata taccttctag
      181 agtacaaaga gaagttattc caaaattatt aaaaggtcaa aacttagtag ttaggtcaaa
      241 aacaggaagt ggaaagacag caagttttgc aataccttta tgcgagaata taaatgtaga
      301 ttacaataat gttcaagctc ttatagtggt tccaacaaga gaattagcct tgcaagtaaa
      361 agatgagata tcagatatag ggagactaaa gaaggtgaga tgtagtgcta tatttggcaa
      421 acaatctata aaagaccaaa tagctgaatt gaaacaaagg gttcatattg ttgtagctac
      481 tccaggcaga atacttgacc atataaatag aggttctatt aagttggaaa atgtaaagta
      541 tttagtaata gatgaggcag ataaaatgtt taataaaggt tttgttgaac aaatggaaaa
      601 aatattatta aatttaccta aggagaaaat agtaagtctt ttttcagcta caatagatga
      661 agagataaaa tatatatgtg aaaaatatat gctagattac agtgttataa atatagaaga
      721 aaatgagagc gatactaatc aaaaaacaag acaaatagat gacaaaataa tcaaggcaaa
      781 tggaagagag aagtacatcc ttttaaaaga attaatatac agcgaaaatc caaagagtgt
      841 gataatattt tgtaatacca aagagaaagt atctaaatta tataataaga tgagtaaaga
      901 aggtttttta ataagagagt tacatgcaga tttatcacaa gaaagaagaa tttttgtaat
      961 taaggatttt aaaaaccaaa aatttaatat attagttagt agtgatgtag cgtccagagg
     1021 aatacatatt gatgatattt ctttagtaat aaactatgat gtgccacaag ataaagaaaa
     1081 ctatatacat agaataggta gaactgggag aaaaggaaat agtggaaagg caattactat
     1141 agtaactgaa aaggatgaaa aatatataga gaatatagaa acgtatatag gatataaaat
     1201 aaatgaatta aaagatatag aacaaagtag aattacacat ggcaaagttt tgtttgaaga
     1261 atattcaaaa ggagtactaa aagaagtatc aaagagaaaa aaagttgata aaaattctta
     1321 caaaagtaaa gatataaaac taaatataga aagtgaagtt gttaaattat atttaaatgc
     1381 tggtaagaag aagaaaataa gagttttaga tatagttggt gcatttagta atataaaagg
     1441 tataactaat gatgatatag gtgtaattga agttcaagat ttatgttcat atgtagatat
     1501 attaaattat aagggagatt tgatattaaa aaaatataag gagataccta taaagaaaaa
     1561 aatggttaaa gtgaaaagag atatacgata atttagtcaa tattgatgaa ttgctttgtt
     1621 atcgaaatga agttgcaaaa aacacaatag aagttgttaa ggatttgaaa gctgaagata
     1681 tgaaacgaaa ggtctctcca attgacttag aaaggatttt ggtgactgga ggagtaataa
     1741 agcatgaaga ttctatttgg ttgttggact tttgggggaa caaagatatt gctggaattt
     1801 tgcttatgcc tcctactcgt catgttatac ttcatttgaa tgattgttgt aaatggaagg
     1861 aatattttcg tacaaaaaag aaattttaca gaagttaaat tataataaat tagacaaaca
     1921 atagataaat tagcaaggaa tgttgatttt atctatattc taagttatac tatttttaag
     1981 tggctaaggg aatagtcatt ttaaatataa gtaataaata tatacctata gggatattaa
     2041 aggggggaaa tatatggcat attttaacta taacgacaaa cagtgctact ataatgaaat
     2101 tggggatggt acaccattac tttttttaca tggaaataca gcctcttcca agatgtttaa
     2161 tgatataatc gatttttata aagatgaata taaagtaata ttaattgatt ttgtaggtca
     2221 tggaaaatct caaatggtcg ataaattttc tgctgactta tggtttgatg aggctatgca
     2281 agtgatatgt tttttagaag caatgaacta taaaaaagta aacataattg gtagtagtgg
     2341 aggcgctctt gttgcattaa atgttgcact tgagagacct gatttagtga ataaggtaat
     2401 tgcagatagt tttgaaggag aagttcctct agagtcattt gtacaaaatg tgaaaataga
     2461 gcgtgaagca tctaagcaag atgatggtgc aaaagctttt tatatttata atcaaggaga
     2521 aaactgggaa aaagttgtag ataatgatac agaagctatt tttgaacatt ataaaactat
     2581 aggaaagttt tttcataagc ctctagaaac tatgcaacca gaggtgcttt taacaggaag
     2641 tagagaagat gagtttgtat ctctaatatc tagtgatttt tttgaaaata ctttttcatt
     2701 acttcttgaa aagattaaaa atggtaaaat gtatttgttt gataaaggtg gtcatcctgc
     2761 aattttatcg aatggattag atttttcaaa tgtagcaaag aaatttttag aggaataatt
     2821 taaccaaaat ttacaataat gaaaactgta aaatggattg caaaacttac aaaagtgagt
     2881 gtacatatac ttcattatta tgatgaaata gtattgttaa aacaaaatat tctaatagtc
     2941 aagttattac atttgtatat gcaatatgta tagaaagaga tatcattaga ttgtaaagtt
     3001 gggattaaat aacaatatta aattttattt attcagatat tttagcttaa ctatataaat
     3061 gttagaacaa atttatatag ttaagctgta ggattaaata atttagttaa ataagtttat
     3121 tatctcctca tcagatagac tttttaatac accttcatta gaaagttctc catttataaa
     3181 ttgatttata agctcttttt tactttcttg taattttatt attttttctt ctatagtccc
     3241 tttagcaatc aattttataa cttgtactga atttttttga ccaaatctat gagctctatc
     3301 actagcttga ttttctactg atggattcca ccatgggtca aagtgaatta ccatgtcagc
     3361 agatgtaaga tttaaaccag ttccgcctgc ttttaaagat attaaaaaaa cttttttatc
     3421 catactatta ttgaactcat ctacaagttc aagtctttct ttggcattag ttttaccatc
     3481 tatataatgg tatttaattt tatacttatc aagttcctta cttatgttct taagcacaga
     3541 agtaaattgt gaaaacaata atattttgtg gttttcgttt atactatccc taagtatttc
     3601 taaacatgtt tcaatttttg aacttttttt attgtaatct tttactacta tacttgggtc
     3661 taaacaaagt tgtcttagtt tagttaaata agaaaatata actattttat ctttctttaa
     3721 atttttatcc ttcattttat cttgtatatc ttttgaatat acactatata tctttttctg
     3781 ctctttattc aattcaacaa aaaagttttt ttcaatctta tctggaagtt ctttcataac
     3841 ttgtttttta cttcttctaa gaataaaagg ttttatcaat tttttcaggt ttttaacatt
     3901 atcttcttta tttataaaca attcctgaaa ctttgcttta ctatatagat agccaggcat
     3961 tataaaatca aatatagacc ataattctag tagattattt tccataggtg taccagtgag
     4021 tgcaaattta ttttctgcat ttatactttt tacagactca gttactaaag ctactggatt
     4081 ttttatattt tgagcttcgt ctaatataca ataatcaaac tttatttcac tatatttttc
     4141 taaatcattt ctaagagtac catatgttgt aagtattaca tcaaaatttt ctaattccat
     4201 aaaacatttt tctctatctc ttttatttcc atgtatcaat aaaactttta tatctggagc
     4261 aaacttttca aattcatttt tccaattata aatgagtgat gtcggtgtaa caataagact
     4321 ttttttatta gataatgata ataaaaatgc aatagtctgt attgttttac caagacccat
     4381 ttcatcagct aatataccac caaatttata ataatctaaa actttaaacc aattgaggcc
     4441 atttatttga tagtcacgaa ggtttgcatt taaatttgcg ggaatttcta aatttatgct
     4501 atcaaaattt ctaaattttt cacatatact gtctagttct tttttaccat ctataaactt
     4561 taaattttta ctacgtatta catcatttat atatagagct tta
//
LOCUS       DAELLV010000261         4579 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.261, whole genome shotgun sequence.
ACCESSION   DAELLV010000261 DAELLV010000000
VERSION     DAELLV010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4579
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.261"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1051
                     /locus_tag="KPD58_003280"
     CDS             <1..1051
                     /locus_tag="KPD58_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG0267009.1"
                     /translation="ADNFIIIMKYIEEEDITKRLKTIFEELDLFNNNQEEKYKLVLSC
                     GIYFILPEDRDINSIIDRANIPHKMAKGGHKSSYAFYDNKIHDQEIKEKEMENTMFSS
                     LENKEFIIYLQPKIELNTGEIQGSEALVRWKRPDKGLIPPNEFIPFFERNGFVINLDL
                     YVLEEVCIYLRKWIDAGINPVTVSVNVSRIHLYCNNFIETYKNIIDKYNIPAKYIELE
                     LTESIIFDNFDILIDIMNNLKKIGFLISMDDFGSGYSSLNMLKEIPMDILKLDQKFIM
                     ETYNSKRSKIIVTKVIEMAKELGMKVISEGVETEEQFKLLKEVKCDMAQGYLFGKPMP
                     IEEFEHLIVSNLIRG"
     gene            1227..1595
                     /locus_tag="KPD58_003281"
     CDS             1227..1595
                     /locus_tag="KPD58_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0267010.1"
                     /translation="MEWELDNNKPIYIQLVEHLKLKIISGEIKIGSKLETVRALAEDA
                     EVNPNTMQKALTELERQGLVYSQRTKGRFVTDDKEKIKAMKEEIANVEINTLKITLEK
                     LGYDRDEMLKLITENLKGEL"
     gene            1597..2322
                     /locus_tag="KPD58_003282"
     CDS             1597..2322
                     /locus_tag="KPD58_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0267011.1"
                     /translation="MDKNISNVSQDGIVEFKGVNKSYGTKNVLKNIDLNIPKGKIVGL
                     LGPNGSGKSTMIKLMNGLLQPDNGEIMINGMKPSIDTKKIVSYLPERTYLNDWMKVSD
                     LLKFFYDFYSDFDVRRANEMIKSLDIDVNEKLKTMSKGTKEKVQLILVMSRNASIYIL
                     DEPIGGVDPAARSYILKTILKNYSEDSTLLIATHLISEIENICDEIIFISKGEIVLQG
                     DVEAIREEKGKSIDALFREEFKC"
     gene            2316..3080
                     /locus_tag="KPD58_003283"
     CDS             2316..3080
                     /locus_tag="KPD58_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0267012.1"
                     /translation="MLGKLLKYELKASGRIFIPLYIAILIVAVFNGIFMNTNILQVQG
                     IGILVLTSLFMALGVLTIVVTIQRFRKNLLGDEGYLMFTLPVSTSSLILSKCITALIY
                     AVLSFIVAVFTFGVLMLFGTSGILLPEILDLFNTSFKWISENFLDILLLVVVMFISYS
                     SFILLLYTSISMGQLPKFNKHRNIVAFASFIAINIVISIVGDAVGSILPNEDTNMVYH
                     LYQSPSFMLAILGSLVVAIALFFATKFILDKKLNLE"
     gene            3302..4552
                     /locus_tag="KPD58_003284"
     CDS             3302..4552
                     /locus_tag="KPD58_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0267013.1"
                     /translation="MKSYTKAKWSVWGIITFSFVLVLFLRMSTAVVSDNLANELGFNS
                     IQISNIASFCLYAYAFMQIPAGILIDKYGARKISSLGIIMASLGSILFGLIQSIELAY
                     ISRVIVGAGTSVILLCILKIQGRWFNKSEFASATAKFSFVGNLGGVLATFPLVFLSEL
                     VGWRNSFLLIGIIGVVIGCFMYIIVRDTPKEYGFNVDTEPYENSEKVNIVDGIKSVIK
                     NKSTWYNSMIMFSFVGLTSAFISLWGVRYIMDVYGVSKSFSAFIVSFFTYGFIFGSII
                     MDFVFAKIRSSKFNIIKFGAMIDLFIWIVIVVVYQVKPPIIVLPISFFIMGCIVMSHL
                     QVFNDAKYKNKEIYSGLATSVINTFEFIGSGIINLIIAISLQVNSYNIVNGYKKGFVV
                     FIVLSIITIVSSHIGVKNDDFKAI"
BASE COUNT         1746 a          431 c          797 g         1605 t
ORIGIN      
        1 tgcggataat tttattataa ttatgaaata tatagaagaa gaagatataa caaagaggtt
       61 aaaaactata tttgaagaat tagacttgtt taataataat caagaagaaa aatataagtt
      121 agttttaagt tgtggaatct attttatact tccagaagat agagatatca actcaatcat
      181 agatagggct aatatacctc ataaaatggc aaagggagga cataaaagtt catatgcatt
      241 ttatgataat aagattcatg accaagagat aaaagaaaaa gagatggaaa atacaatgtt
      301 ttcaagtcta gaaaataaag aatttattat atacttacaa cctaagatag aacttaatac
      361 aggagagata caaggttcag aagcacttgt taggtggaaa cgtcctgata aagggctaat
      421 acctccaaat gaatttatcc cattttttga aagaaacggt tttgttataa atttagattt
      481 atatgtgctt gaagaagtat gtatatacct tagaaaatgg attgatgcag gaataaatcc
      541 agttactgtt tcagttaatg tttcaaggat acatttatat tgcaataatt ttatagaaac
      601 atacaaaaat ataattgata aatacaatat acctgcaaag tatatagaac ttgaattaac
      661 ggagagtatc atatttgata attttgatat tttaattgat ataatgaata atttaaaaaa
      721 aataggattt ttaatatcta tggatgattt tggttcaggg tactcatcat taaatatgtt
      781 aaaagaaatt cctatggata tcttaaaact tgaccaaaaa tttattatgg aaacttataa
      841 tagtaaaaga agcaaaataa ttgttactaa agtaatagaa atggcaaaag agttgggtat
      901 gaaagtcata tcagaaggag ttgagacaga agaacagttt aaacttttaa aagaagtaaa
      961 gtgtgatatg gcacaaggat atttatttgg aaagccaatg ccaattgaag aatttgaaca
     1021 tttaattgta tccaacttga taagagggta attaatctgt aatataaaga atttaataaa
     1081 ttgtgttaaa aatggactct aatgtagtgt tcattttttt tgtaaaattt tttatacttt
     1141 tactaaaaag taattgacat atactttgta ttattatatt attgtattaa caacttaata
     1201 caataatata ataaggaggc atgaacatgg aatgggaact tgataataac aaacctatat
     1261 atatacaatt agtagaacat ttaaagttaa aaataatttc aggggaaatt aaaataggat
     1321 ctaaattaga aacagtgaga gcactagcag aagatgcgga agttaatcca aatacaatgc
     1381 aaaaggctct aacagaactt gaaagacaag gtttagttta tagtcagaga actaaaggaa
     1441 gatttgtaac agatgataag gaaaaaataa aagctatgaa agaggaaata gctaatgtgg
     1501 agataaatac attaaaaata accttagaaa agcttggata tgatagagat gaaatgctaa
     1561 aacttattac agaaaatctg aaaggagaat tataaaatgg ataaaaatat aagcaatgtt
     1621 tctcaagatg gaatagttga atttaaaggt gtaaacaaga gttatggaac taagaatgtt
     1681 ttaaaaaata ttgatttaaa tataccaaag ggaaaaatag tcgggctgtt aggacctaat
     1741 ggaagtggca aaagtacaat gattaagcta atgaatggat tattacagcc tgacaatggt
     1801 gaaattatga taaatggaat gaaaccatcc atagatacta aaaaaatagt ttcatattta
     1861 ccagaaagaa cttatttaaa tgattggatg aaggtatcag atttacttaa gtttttctat
     1921 gatttttata gtgattttga tgttagaaga gcaaatgaga tgattaaaag cttagatatt
     1981 gatgtaaatg aaaagctaaa gacaatgtct aaaggaacta aagaaaaagt tcaacttatt
     2041 ttagtaatgt caagaaatgc gagcatatat atattggatg aacctatagg tggtgttgac
     2101 ccagctgcaa ggtcatatat acttaagact atattaaaaa attattctga agatagtact
     2161 ttactgattg ctacccactt aataagtgaa atagaaaaca tatgtgatga gataatattc
     2221 atatcaaaag gagaaatagt gttacaagga gacgttgaag caataagaga agaaaaagga
     2281 aaatctatag atgcattatt tagggaggaa tttaaatgtt aggtaaatta ttaaaatatg
     2341 agttaaaagc aagtggaaga atatttatac cgttatatat agccatattg atagtagctg
     2401 tatttaatgg aatatttatg aatacaaata tacttcaggt acagggaata ggaatactag
     2461 tattaacaag tctttttatg gcattaggag ttttaactat tgtagttaca attcaaaggt
     2521 ttagaaaaaa tcttttgggg gatgaaggtt atttaatgtt tactcttcct gttagcacta
     2581 gttcacttat attatctaaa tgtataacag cacttatata cgctgtactt agttttatag
     2641 ttgcagtatt cacttttgga gttcttatgc tttttggtac ttctggaatt ttactacctg
     2701 aaattttaga tttgtttaac actagtttta aatggatttc tgaaaacttt ttagatattt
     2761 tattattagt agtagtaatg tttatttcat attcatcatt tattttgtta ttatatacat
     2821 caatctcaat gggacaatta ccaaaattca ataaacatag aaacatagtg gcatttgctt
     2881 cttttatagc tataaatata gtaatatcaa ttgttggaga tgcagttgga agcatcttac
     2941 caaatgaaga tacaaatatg gtatatcatc tttatcagag tcctagtttt atgcttgcaa
     3001 tacttggtag tcttgtagtt gcaatagcgt tatttttcgc tacaaaattt atattagata
     3061 aaaaattaaa tttagaataa atgtttttat atggagccat cttttgagta atgttaaaag
     3121 atggctctta ttatttatgt agttaattca tgtatataat atgaatagtg ttttatttct
     3181 agttaaatat atacatatgt tgtataatga tattgcgtgt aaaaatttgt aagaataact
     3241 atacacttta tataagttat atcaagaaaa tttaaaaata tatggatttg agggagaaga
     3301 tatgaaaagt tatacaaagg ctaagtggag tgtttggggt ataattacat tttcatttgt
     3361 tttagtatta tttttaagga tgtcaactgc tgttgtttca gacaatttag caaatgagtt
     3421 aggttttaat tctatacaaa tttcaaatat agcatccttt tgcctatatg catatgcatt
     3481 tatgcaaata cctgcaggaa ttttgattga taaatatgga gcaagaaaaa ttagcagtct
     3541 tggaataata atggcaagtt tagggtcaat tttatttggt ttgattcaaa gtatagaatt
     3601 agcatatatt tctcgtgtaa ttgtaggggc aggaacatca gtaatattac tttgtatttt
     3661 aaaaattcaa gggagatggt ttaataagtc agaatttgct tctgcaacag ctaaattttc
     3721 atttgtagga aatttaggtg gagttcttgc tacttttcca cttgtgtttt tgtctgaact
     3781 tgttggttgg agaaattcat ttttattaat cggtataatt ggtgttgtaa ttggatgttt
     3841 tatgtatatt attgttagag atacaccaaa agagtatgga ttcaatgtag atactgaacc
     3901 ctatgaaaat tcagagaagg ttaatattgt tgatggtatt aagtctgtta taaagaacaa
     3961 atcaacatgg tataattcaa tgataatgtt ctcttttgtg ggtcttacaa gtgcatttat
     4021 aagcttgtgg ggagtaagat acataatgga cgtttatggt gtgagtaaga gtttttctgc
     4081 gtttatagta tcctttttta catatggatt tatatttggt tcaataatta tggattttgt
     4141 atttgctaaa ataaggtcta gcaaatttaa tataataaaa tttggggcaa tgatagattt
     4201 gtttatatgg attgtaattg tagttgtata ccaggtaaaa ccaccaatta tagttttacc
     4261 aatatcattc tttattatgg gatgtatagt tatgtcacat ctacaggttt ttaatgatgc
     4321 caagtataaa aataaagaaa tttattctgg actggcaact agtgttatta atacttttga
     4381 atttatagga agtggaatta ttaatttgat tatagctatc tctttacaag taaattcata
     4441 taatatagtt aatggatata aaaaaggttt tgtagtattt atagtactaa gtattataac
     4501 tatagtatca tctcatatag gggtaaaaaa tgatgatttt aaggctatat agtgttttat
     4561 taatagttta atttagtat
//
LOCUS       DAELLV010000262         4525 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.262, whole genome shotgun sequence.
ACCESSION   DAELLV010000262 DAELLV010000000
VERSION     DAELLV010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4525
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.262"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..571)
                     /locus_tag="KPD58_003285"
     CDS             complement(<1..571)
                     /locus_tag="KPD58_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBG0267014.1"
                     /translation="MKKFLNVLFSICISIVIIVGVINFTVGFKQLYYFDIDYLNISEL
                     SGLSKDDIKLNYDYLIDYNLNKNVSEFKLPTLKSSPQGKIHFEEVRNIFQNINKLAKL
                     LLVVSLVGIILSVKNKNIKILKTTSITLIIMPLLLTVPILLNFEKSFIIFHKLLFRND
                     YWIFNPDLDPVINILPEEFFFHSGMMILIL"
     gene            complement(600..2150)
                     /locus_tag="KPD58_003286"
     CDS             complement(600..2150)
                     /locus_tag="KPD58_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG0267015.1"
                     /translation="MLVVENVSHGFGARTILENVSFRLRKGEHIALVGANGEGKSSFL
                     NIITKKLMPDAGNIKWSSRATVGYLDQHTVLSKGKTIREVLREAFKHMFDLEQEMIAM
                     YDKMGEASDDEMSKLLEETAEIQTILENSGFYMIDAKIQEVANGLGLGEIGLDKDVTD
                     LSGGQRTKVLLTKLLLENPTILILDEPTNYLDEEHITWLTKYLQEYENSFVLVSHDIE
                     FINNTCNVIYHMENGELNRYKGNYDEFVRLNDIKKRQEEQAYDKQVEERKRLEDFVAR
                     NKARVATRGMANSRQKQLDKMEILERPKEKIKPTFAFKDARAASKIIFETENLVLGYD
                     EALTKPLNFHLERGKKIALKGMNGIGKSTLLKTLLGIIKPFEGNVKLGDYLEVGYFEQ
                     ESSRENSNTPMDEVWSEFPGLTNFEVRQALAKCGLTNEHITSQMRVLSGGEAAKVRLC
                     KVMLKNINFLVLDEPTNHLDVEAKDELKKAIKEFKGTVLLVCHEPEFYSEIVDDVWNI
                     EDFTTKIV"
     gene            complement(2352..3236)
                     /locus_tag="KPD58_003287"
     CDS             complement(2352..3236)
                     /locus_tag="KPD58_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG0267016.1"
                     /translation="MKKYRLLVTCIIAMGIIIIGTSNPVSNFYKTTNLISSGNITNEK
                     ADKDENSNKNNKDKNEDKSNSKSNKDKSSEDKSSDNKNEIQKNKKLLICIDPGHQGKG
                     DSNLEPVAPGSSSKKARVSSGTEGIATKKPEYVLNLEASLVLKSILESKGYNVIMTRE
                     THDVNISNSERAILANDKKADMVVRIHADSLNNSSKTGASILIPEKDGKYTAPIYEES
                     NKCAEFIKQNMEQSGIQINGIVQRGDLTGFNWSKVPAVLVEMGFMSNYNEDQMMSNPD
                     YQRKMMQCIADGLDAYFK"
     gene            3464..4471
                     /locus_tag="KPD58_003288"
     CDS             3464..4471
                     /locus_tag="KPD58_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HBG0267017.1"
                     /translation="MQLKNFHFKNNTLSKKTNLAKSKFTIVLTFLFLLYFFSNNLIYA
                     QNLQKNEPEYLIPIGNVLQIDAELKNIIVRNPGEASPFRLGDAIVKVNNAEVDGYSDF
                     ANTLNALPEEKQVSILLNRGGQLITMKTTKHILEKISFNNLLSGFATLTYINPDTSEF
                     GAVGHPISVGNARKIPIKTGSISTTKHLNIEKSYKGSVGCINAKRENIIGSFTENTDF
                     GIRGQINNFDISKLKKYKVASLDEVKLGKAQIILQDNFNKCKKYNIEIIDIENQKHPE
                     SKSFKIKITDKKLLAQTGGIVQGMSGTPIVQDNKIIGAVSHAIENDPAVGYGVFIKWM
                     L"
BASE COUNT         1527 a          729 c          516 g         1753 t
ORIGIN      
        1 ttaatattaa aatcatcatt ccagagtgaa aaaaaaattc ctctggcaat atatttataa
       61 ctgggtctaa atcgggatta aatatccaat aatcatttct aaataataat ttatgaaaaa
      121 taataaatga cttttcaaaa tttaataaaa ttggaactgt taataataat ggcataatta
      181 taagtgttat tgatgtagtt tttaagattt ttatattttt attttttaca ctcaatataa
      241 ttcctactaa agaaactacc aataataatt tagctaattt atttatattt tgaaatatat
      301 tcctgacttc ttcaaaatgt atcttccctt gtggagaaga ttttaatgtt ggtaatttga
      361 actcagacac attcttattt aagttataat caattagata atcgtagttc aattttatat
      421 catctttaga taaaccagat aactctgata tatttaagta atcaatgtca aaataataaa
      481 gttgtttaaa tccaacagta aaattaataa cacctactat tataacaata ctaatacaaa
      541 ttgaaaaaag tacatttaga aactttttca tgaaatcccc ccagcaataa gttaaatcac
      601 tacacgattt tagttgtaaa atcttctata ttccatacat catcaacaat ttctgaataa
      661 aattctggct catgacatac taaaagaact gttcccttaa actctttgat agccttcttc
      721 aattcatctt ttgcctcaac atctaaatga tttgtaggct catctaatac taagaaattt
      781 atatttttta acattacttt gcaaagtcta accttagcag cttctccacc acttaatact
      841 ctcatttgac tcgttatatg ttcatttgta agtccacatt ttgcaagagc ctgtctaact
      901 tcaaagtttg taagccctgg aaattcactc caaacttcat ccataggtgt attactattt
      961 tctcttgaac tctcttgttc aaaatatcca acttccaaat agtcacctaa tttaacattt
     1021 ccttcaaatg gttttattat tccaagtaaa gtttttaata gggtagattt tccaatacca
     1081 ttcattcctt ttaaggcaat cttttttcct ctttctaaat ggaaattaag tggttttgtc
     1141 aatgcttcat cataccctaa aactaaattt tctgtttcaa aaattatttt actagctgct
     1201 ctagcatctt taaaagcaaa agtaggtttt attttttctt ttggtctttc taatatttcc
     1261 atcttatcaa gttgcttttg tctactgtta gccattcctc tagttgctac tctagcttta
     1321 tttctagcta caaaatcctc tagtcttttt ctttcttcaa cttgtttatc ataagcttgt
     1381 tcttcttgac gtttctttat atcattaagt cttacaaatt catcataatt tcctttatat
     1441 ctatttaact ctccattttc catatgatat attacattac atgtattatt tataaattca
     1501 atatcatgag aaactaagac aaaactattc tcatactctt gaaggtactt agtaagccat
     1561 gttatatgct cttcgtctaa atagtttgta ggctcatcca atataagaat agttggattt
     1621 tcaagtagaa gtttagtaag aagtactttt gttctttgcc ctccacttaa gtcagttaca
     1681 tctttatcaa gaccaatttc tccaagacca agaccatttg caacttcttg tatctttgca
     1741 tctatcatat aaaaaccact attttcaagt atagtttgta tctctgctgt ttcttcaaga
     1801 agcttactca tttcatcatc actagcttct cccattttgt catacatagc aatcatttct
     1861 tgctctaagt caaacatatg cttaaaagcc tctctaagca cttctcttat agtttttccc
     1921 ttgcttaata ccgtatgttg gtctaagtaa cctactgtag ctcttgaaga ccattttata
     1981 tttccagcat ctggcataag cttctttgtt attatattta aaaaactcga ttttccttct
     2041 ccattagcac caactagtgc aatatgttca ccttttctaa gtctaaagga tacattttct
     2101 aatattgttc tcgcaccaaa tccatgactc acattttcta ctactagcat ttcaacacct
     2161 ctcaaaaaaa ttttacacat tctatactat aatatattct ttttaaactt aaatcaagta
     2221 ttcccaatat ttttagttat atttacgaca atttaaccta aaataaactt cttctttgtg
     2281 ttatgtacaa taaaaaatag agataaaaat taggaaaata ataccaacct aactttttct
     2341 ctattcatat cttatttaaa atacgcatcc aatccatcag ctatacactg catcattttt
     2401 ctttggtagt ctggatttga catcatttgg tcttcattat agttactcat aaatcccatt
     2461 tctactaata cagctggtac ttttgaccag ttgaatccag tcaaatcgcc tctttgaacg
     2521 attccattga tttgtatacc tgattgttcc atattctgtt tgataaattc agcacattta
     2581 ttactttctt catatatagg agctgtatat tttccatctt tttcaggaat caatatagat
     2641 gctccagttt ttgaagaatt atttaaacta tctgcatgta ttcttacaac catatctgct
     2701 tttttatcat tagcaagtat tgctctttct gaattactta tatttacatc atgtgtctct
     2761 ctagtcatta ttacattata acctttagat tctaatatac tctttaaaac aagagatgct
     2821 tctaagttta aaacatattc tggttttttt gtagcaatac cctctgttcc tgaagatact
     2881 ctcgcttttt tagaggaaga acctggagct actggttcta aattgctatc tccttttcct
     2941 tgatgccctg ggtctataca aattaaaagt tttttatttt tttgaatttc atttttatta
     3001 tctgacgatt tgtcctctga tgacttatct ttattacttt tagaattaga tttgtcttca
     3061 tttttatctt tattattttt gtttgaattt tcatctttat ctgctttttc atttgtgata
     3121 tttcctgaac taatcaaatt tgtagtcttg taaaaattgc taactggatt tgatgttcct
     3181 ataattatga ttcccatggc aattatacaa gtaactaaca atctatattt tttcattttt
     3241 tgctcctttc ttatgctaga cagttattgt ttatttttat ttatatgaat atgtatttat
     3301 tttatcatat acactattta ttttatttat ttttatgaaa actaaggtgt tattgagcta
     3361 gttttccaag catcaaaaca tgattcttgc acaacataat atagaagcag caagaaaact
     3421 tcaattctat tattaaaata atttaataag aaagaggtgt tttatgcaac ttaaaaattt
     3481 tcattttaaa aataatactt taagtaaaaa aaccaactta gctaagtcaa aatttacaat
     3541 tgttttaaca tttttatttt tattatattt tttctcaaat aatttaattt atgcacaaaa
     3601 tttacaaaaa aatgaacctg aatatttaat accaattgga aacgtacttc aaattgacgc
     3661 agaattaaaa aatatcatag ttagaaatcc tggagaagca tctccattta gattgggtga
     3721 tgctatagta aaggtaaata atgctgaagt tgatggatat agtgactttg ccaatacctt
     3781 aaatgcttta ccagaagaaa aacaagtatc tattttatta aatagaggtg gacaacttat
     3841 aacaatgaaa actaccaaac acatattaga aaaaattagt tttaacaatt tactttctgg
     3901 atttgctact ttaacgtata tcaatccaga tacatcagag tttggagctg taggtcatcc
     3961 aataagtgtg ggtaatgcaa gaaaaatacc tattaaaaca ggttcaattt ctacaactaa
     4021 gcaccttaat atagaaaaat cttataaagg tagtgttgga tgtataaatg caaaacgaga
     4081 aaatattatt ggtagcttta cagaaaatac tgattttgga ataagaggtc aaattaataa
     4141 ctttgatatt tccaagttaa aaaaatataa agtagcatct ttagatgaag ttaaattagg
     4201 aaaagctcaa ataattttac aagataactt taataagtgt aaaaaatata atattgaaat
     4261 tatagatata gaaaaccaaa aacatccaga aagtaaaagt tttaaaatta aaattacaga
     4321 taaaaagctt ttagctcaaa ctggtggtat tgtacaaggt atgagtggta ctccaatagt
     4381 tcaagataat aaaataatcg gagcagtgtc acatgcaatt gaaaacgacc cagctgtagg
     4441 ttatggtgta tttattaaat ggatgttgta aataatataa ataaataagt tttaagatgt
     4501 tattaagtta ttaataatat atttg
//
LOCUS       DAELLV010000263         4495 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.263, whole genome shotgun sequence.
ACCESSION   DAELLV010000263 DAELLV010000000
VERSION     DAELLV010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4495
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.263"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     misc_binding    119..353
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            403..1422
                     /gene="pheS"
                     /locus_tag="KPD58_003289"
     CDS             403..1422
                     /gene="pheS"
                     /locus_tag="KPD58_003289"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBG0267018.1"
                     /translation="MQEKLLALREAALAEIKEAQSIESVESLRVKYLGKKGEITAILK
                     EMGKLSAEERPVVGKVANEVRENIELSINSKKEEINAIEKERKLKEEVIDVTQPGKVL
                     KVGKKHPITQIIDEVTDIFIGMGFSIAEGPEVETVENNFDALNAPKDHPSRDMSDTFY
                     INDGVLLRTQTSPVQVRTMRSQELPIKVIAPGRCFRSDSPDATHSPMFHQIEGLVVGK
                     DVTMAEFKGTMDIFVEKLFGSDIKTKFRPHNFPFTEPSAEVDVTCFKCGGKGCPMCKY
                     EGWIEILGSGMVHPNVLRNCGIDPEVYSGFAFGVGVERLAMLKYEIDDIRLLFENDMR
                     FLNQF"
     gene            1439..3832
                     /locus_tag="KPD58_003290"
     CDS             1439..3832
                     /locus_tag="KPD58_003290"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBG0267019.1"
                     /translation="MLVSLKWLRDYVDIDMDVKEFADKMTMSGTKVETIDYYGEEIEN
                     ILVGKILEIKQHPNADKLVVTKVDIGDKVVQIVTGATNISEGDYIPVAVNGSKLPGGV
                     EIKQTDFRGELSDGMMCSAAELGIDEHYIEEYKRGGIYILDHEDSYELGKDIKDVLGL
                     KDALIDFELTSNRPDCKCMMGIAREAAATIGTKVKYPEIEVKESDEEIDFKVEIDNPD
                     LCRRYVARMVTDVKIEPSPYWMQRRLTEAGVRPISNIVDITNFVMLELGQPLHAFDIN
                     QVETGRIVVRNAKDGEKLVTLDDVERTLDKDMLVITNGEKSLGLAGVMGGANSEITSN
                     TKTVLFESANFKPENIRMTAKKVGIRSEASSRNEKDLDPNLAEIAANRAAQLVEMLGA
                     GKVLKGVVDVYPNKPEPKKLVVNPQRINHLLGVDVPMEQFVGILESLEFKCNLVANDK
                     LEIDVPSFRTDMEQEADVWEEIARIYGFENIPSVQLEGNTTAGIKTSKQKFMDALKDN
                     STAVGLNEILTYSFVSPKGVDKIRVPEGNAKRNFVKLLNPLGEETSVMRTTLIPNMLD
                     VLSTNVSHKIEEVSAFECGHIFIPQDSELPKEENRMCVGMYGKDVDFFALKGTIETIL
                     VNVGFKGYEIEPQDNNTTFHPGRCAKIVYNNKYVGTLGELHPDVIENYNLGQRVYVAE
                     IDIDFVFDNSDRTKNYVPLPKYPSTSRDIALIVKDDVFVKQIEDIIKENGQGLVESYK
                     LFDVYKGSQIEAGYKSIAYSITYRSKDKTLTDEDVAKVHDKILSELSEKLNANLRSN"
     gene            3857..4447
                     /gene="zapA"
                     /locus_tag="KPD58_003291"
     CDS             3857..4447
                     /gene="zapA"
                     /locus_tag="KPD58_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBG0267020.1"
                     /translation="MNKVMVKIHGAEYPMVGDKSEKFMISIADFVDKEMDKITRQNPK
                     LSLSVAAILTALNISDLLFECSDENEKLIKANEELSKKVGASNEELQLEIKSLKLTIA
                     EKEAENREAETKMKELIEIIENKKQEIFELSNTTEGSRAELDAYKNKIEELSTQLEEA
                     NERATIAENLASEFQNKAYDLQLKCTGLNNDAKNVE"
BASE COUNT         1771 a          520 c          922 g         1282 t
ORIGIN      
        1 gcattagatg tatattatat aaaaaataat taaattaatt atgtattgga tatatattaa
       61 aaatagtcac ataatttgaa tggaaatgat ataatactaa aataaacaat ataatattgt
      121 aaatgcaatg aaagaggaaa gtattttgat taaacttagt aaagagataa acacctaggc
      181 tgggagtgtt ttctaagagg tcatgagaag ttccctctgg agtaacagag ctgaaatttt
      241 acagtaggct ttgacgtcaa aaacgcgtta agtttgttag aggtggtttg atgattttta
      301 aattgttaaa ctactagggt ggtaccgcga aactatagcc tttcgtccct agacagggat
      361 tgaggggctt tttttataca aaaaaacgaa agggtgatat gtgtgcaaga aaaattactt
      421 gctttacgtg aagcagcttt ggctgaaata aaagaagcac aaagcataga aagtgtagaa
      481 agtttaagag ttaagtactt aggaaaaaaa ggtgagataa ctgccatact taaagaaatg
      541 ggtaaattat ctgctgaaga aagaccagta gttggtaagg ttgccaatga ggtaagagaa
      601 aacattgaac ttagcataaa ttctaaaaaa gaagaaataa atgctattga aaaagaaaga
      661 aaattaaaag aggaagtgat agatgttact caaccaggaa aagttttaaa ggttgggaag
      721 aagcatccaa taactcaaat tatagatgaa gtaacagata tatttatcgg aatgggattc
      781 tctatagcag aagggccaga agttgagact gttgaaaaca actttgacgc attaaacgct
      841 cctaaagacc atccatcaag agatatgagc gatacattct atatcaatga tggggtatta
      901 cttagaactc aaacatctcc agttcaagta agaactatga gaagtcaaga gttaccaata
      961 aaagtaattg caccaggtag atgttttagg tcagactcgc cagatgctac acactcacca
     1021 atgttccatc agatagaagg gcttgttgtt ggaaaagatg ttactatggc agaatttaaa
     1081 ggaactatgg atatcttcgt tgaaaaattg tttggttctg atatcaaaac taagtttaga
     1141 cctcacaact tcccatttac agaaccaagt gcagaggttg atgttacttg tttcaaatgt
     1201 ggtggtaaag gttgcccaat gtgtaaatat gaaggttgga tagaaatatt aggttcaggt
     1261 atggttcatc caaatgtgct tagaaattgt ggaatagacc cagaagttta cagtggattt
     1321 gcatttggag ttggggttga aagacttgca atgcttaaat atgaaataga tgatattaga
     1381 ttattattcg aaaatgatat gagattctta aatcaatttt aattaggagg gtatgtagat
     1441 gttagtatct ttaaaatggc ttagagacta tgttgatata gacatggatg taaaagagtt
     1501 cgctgataaa atgacaatgt caggaactaa agttgaaaca atagattatt atggtgaaga
     1561 aatagaaaat atattggttg gaaagatttt agaaataaaa caacatccaa atgctgataa
     1621 gttggttgta accaaagtag atattggaga taaagttgtt caaatagtta caggagctac
     1681 aaatatatca gaaggagatt atattccagt agctgtaaat ggttctaagt tacctggagg
     1741 agttgaaatc aaacagactg atttcagagg tgaattatca gatggtatga tgtgttcagc
     1801 agctgaacta ggtatagatg aacattacat tgaggagtat aaaagaggtg gtatatatat
     1861 tttagaccac gaagattctt atgaattagg aaaagatata aaagatgttt taggattaaa
     1921 agatgcttta atagattttg aattaacttc aaacagacct gattgtaaat gcatgatggg
     1981 tatagctaga gaagcagctg caactatagg aacaaaagta aaatatcctg aaatcgaagt
     2041 aaaagaaagt gacgaagaga tagatttcaa agttgagata gataatccag atttatgtag
     2101 aagatatgtt gctagaatgg ttacagatgt aaaaatagaa ccttctccat attggatgca
     2161 aagaagactt acagaagcag gagtaagacc tataagtaac atagtcgata taacaaactt
     2221 cgtaatgtta gagcttggtc aaccacttca tgcttttgat ataaatcaag tagagactgg
     2281 aagaatagta gtaagaaatg ctaaagatgg agagaaactt gtaacattag atgatgttga
     2341 gagaacatta gacaaagata tgctagttat aacaaatgga gaaaaatcac ttggtttagc
     2401 tggtgtaatg ggtggtgcta actcagaaat aacttctaat acgaagactg tactttttga
     2461 aagtgccaat ttcaaaccag aaaacataag aatgacagct aaaaaagttg gtattaggtc
     2521 agaagcatct tcaagaaatg aaaaagactt agaccctaat cttgcagaga tagcagcaaa
     2581 tagagctgca cagcttgttg aaatgttagg agcaggaaaa gttttaaaag gtgttgtaga
     2641 tgtatatcca aataaaccag aacctaaaaa attggtagta aatcctcaaa gaattaacca
     2701 tctattaggt gtagatgtac caatggagca gtttgtagga attttagaat cattagagtt
     2761 taaatgtaat ttggtagcta atgataaatt agaaatagat gtaccaagct ttagaacaga
     2821 tatggaacaa gaagctgatg tatgggaaga aatagctaga atttatggat ttgagaatat
     2881 accttctgta caattagaag gaaatacgac agcaggaatt aaaacttcaa aacaaaaatt
     2941 catggatgct ttaaaagata attcaactgc tgtaggatta aatgaaatat taacatattc
     3001 atttgtaagc cctaagggag tagataagat aagagtacca gaaggtaatg caaagagaaa
     3061 ttttgttaaa ttacttaacc cattaggaga agaaacttct gtaatgagaa ctactttaat
     3121 accaaatatg ttagatgttt tatcaactaa tgtatctcat aaaatagaag aagtatctgc
     3181 atttgagtgt ggacatatat tcatcccaca agattcagaa ttacctaagg aagaaaatag
     3241 aatgtgtgta ggtatgtatg gtaaagatgt tgacttcttt gcactaaaag gaactataga
     3301 aactatactt gtaaatgtcg gatttaaagg ttatgaaatt gaacctcaag ataataatac
     3361 taccttccac cctggtagat gtgctaaaat agtgtataat aataaatatg taggtacatt
     3421 aggtgaattg catccagatg ttatagaaaa ctacaatcta ggtcaaagag tttatgtcgc
     3481 tgaaatagat attgactttg tatttgataa ttctgataga acaaagaatt atgtgccatt
     3541 accaaaatat ccatctacat caagagatat agctcttatt gttaaagatg atgtatttgt
     3601 aaaacaaata gaagatataa taaaggaaaa tggtcaagga ttagtagaaa gctacaagtt
     3661 atttgatgtt tacaaaggtt ctcaaataga agctggatac aagtcaattg catattcaat
     3721 aacttataga agtaaagata aaactctaac agatgaagat gtagctaagg tgcatgataa
     3781 gatattaagt gaattaagcg aaaaattaaa tgctaactta agatcaaatt aacaactaat
     3841 gtcgggggag gttaggatga acaaagtaat ggttaaaatc catggcgctg aatatccaat
     3901 ggttggagac aaatcagaga agtttatgat tagtattgct gattttgtag acaaagaaat
     3961 ggataaaata acacgtcaaa acccgaaatt gagcttatca gttgctgcga tattaactgc
     4021 attaaatata tcagaccttt tatttgaatg ttcagatgag aatgagaagt tgattaaagc
     4081 taatgaagaa ttgagtaaga aggttggagc atctaatgaa gagcttcaat tagaaatcaa
     4141 gtctttaaag cttacgatag cagaaaaaga agcagaaaat cgtgaagctg aaactaagat
     4201 gaaagagtta attgagatta tagaaaataa aaagcaagaa atattcgaac ttagtaatac
     4261 gacagaaggt tctagagcag agttagatgc ttataaaaac aagattgagg agttaagtac
     4321 tcaacttgaa gaagcaaatg aaagagctac tatagctgaa aacttagctt ctgagtttca
     4381 aaacaaagct tatgatttac agttaaaatg tacagggtta aataatgatg caaaaaatgt
     4441 ggaataaact aaaagatttg ctgtctcttg ttttttgaga cagcttcttt taatg
//
LOCUS       DAELLV010000264         4493 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.264, whole genome shotgun sequence.
ACCESSION   DAELLV010000264 DAELLV010000000
VERSION     DAELLV010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4493
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.264"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            344..1204
                     /locus_tag="KPD58_003292"
     CDS             344..1204
                     /locus_tag="KPD58_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG0267021.1"
                     /translation="MKKQKSPILLEMLGLFFGCISMSIGINMFLKPHTIAPGGLSGLS
                     LVLNKVTGLPVSAIMLIIGVPLVILAFRIMGTKNSLKTLFGTVVFSAIVQLTDPLSKL
                     RFTNDLLLSSIAGGILVGIGLGIMFKSDASTGGTDLIALILSKKFPGIKATKFMSCLD
                     GMVVISSGIVNRSLETALYSGIALCVLIKIADMIMEGFDHSKAFFIISDEPESLRQAI
                     TEELDRGLTILDGKGGYTRENKEVLLVVVSKKQELYLKRLVKRTDPTAFVIVTDVHEV
                     LGEGFKNLEN"
     gene            complement(1443..2000)
                     /locus_tag="KPD58_003293"
     CDS             complement(1443..2000)
                     /locus_tag="KPD58_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0267022.1"
                     /translation="MSDSEYPQMKTLETRSCILRPASLDDVEDFFDCYKNKIVVKHLP
                     FSEHKSLEDTRKFIKSFFLNPYKKGKIGHFAIVYKRDNKVIGNMGFNNINPKALEAEI
                     GICINPSYWGHDFATEITKRVIQYGFRELNLNKIIAITYEENQNSKKSLDLLGFKCTG
                     KYIKKIHTGNTVKNVPCYQYELKKQ"
     gene            complement(2190..3077)
                     /locus_tag="KPD58_003294"
     CDS             complement(2190..3077)
                     /locus_tag="KPD58_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG0267023.1"
                     /translation="MNIKLELYKVFNAVVNNKSFSLAAKELFMSQPAVSQSIKQLEEQ
                     LDTLLFYRNNKGVKLTPEGKILSEHVTTALKLISSGEDRINKFKKLEYGSLKIGVGDT
                     AARFFLLKYLEIFHKKYPHIHVSTINRTSRELISLLKDGNIDIAIINMPIEDDTLNIV
                     ECIEIHDIFVCANDYIEYKGRKISLEELNTLPLIMLENKANSRLYVNEYFLSKGIKLN
                     PDIELGSHELLLEFAYINLGVSCVIEEFSIDYLENEKLFKLDIKEPIPKRNIGYCHLK
                     DISLSLATKEFLSMISNNI"
     gene            3196..4395
                     /locus_tag="KPD58_003295"
     CDS             3196..4395
                     /locus_tag="KPD58_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HBG0267024.1"
                     /translation="MDRLVGTVSRGVRAPIIRQGDDLVKIVVDSVLNASKSENFEVRD
                     KDVIAVTEAVVARAQGNYAHVDNIAKDVKDKFGDDTVGVIFPILSRNRFAICLKGIAK
                     GCRKVVLMLSYPSDEVGNHLISIDELDDKGINPWSDVLTEEKYRELFGYNKHTFTGVD
                     YVDYYKNLIKDCGAEVEIVFANNPKTILNYTTSILTCDIHTRQRTKRILRQNGAKKVY
                     SLDDIMTASVDGSGYNDQYGLLGSNKATEETVKLFPINCDEVVNKIQGNIKEITGKDV
                     EVMVYGDGAFKDPVGKIWELADPVVSPAYTKGLEGTPNEVKLKYLADNDFADLSGDEL
                     KEAISKYIVEKDNKSDDLTGNMVSQGTTPRRLTDLIGSLADLTSGSGDKGTPIIYIQG
                     YFDNYTK"
BASE COUNT         1638 a          578 c          634 g         1643 t
ORIGIN      
        1 ttaataaatc attttgataa catataaaag taagataaat ttttccatta aataccttat
       61 aattatacac aaaatctcta tatacgttaa acagtataga gaaaattttc atagaaccat
      121 ctttataggg gatactttat aaaagaataa aaaaattata atatataaaa acttcatagg
      181 agtgtaacaa catcagtaaa aatatgaaaa cactatataa aaattcaaaa atattaagaa
      241 taatgaaata cttgaaattg cttatatcaa gaaattttat ataatataaa ctggtcaaat
      301 atgttataat attaaattgt gacaagttca tggaggtgtt tatatgaaaa aacaaaagtc
      361 gccaatttta cttgaaatgc ttggactatt ttttggttgt atatcaatga gtattggtat
      421 aaatatgttt ttaaaaccac atacaattgc tcctggagga cttagtggtc tatcattagt
      481 actaaacaag gttacaggac ttccagtatc agctatcatg ttaattatag gtgtcccatt
      541 agtaatactt gcttttagaa ttatgggtac aaaaaattca ttaaaaactt tatttggaac
      601 agttgtattt tcagctatag ttcaattaac agacccatta tcaaaactaa gatttactaa
      661 tgacttatta ttgtcttcta ttgcaggagg aatcctagtt ggtataggtt taggaataat
      721 gtttaaatca gatgcttcta ctggaggaac agacttaatt gcactcatac ttagtaagaa
      781 attcccagga ataaaagcta caaaattcat gtcatgccta gatggaatgg ttgttatatc
      841 ttctggtata gtaaatcgta gtttagaaac tgctctttac tctggtatag cactttgtgt
      901 attaataaaa atagcagata tgataatgga aggatttgac cattcaaaag cattttttat
      961 tatctctgat gaacctgaaa gtttaagaca agctataact gaagaacttg atagaggtct
     1021 tactattctt gatggtaaag gtggatacac tagagaaaat aaagaagttc tattagtcgt
     1081 agtttctaaa aaacaagaac tttatctaaa gagattagtt aaaagaactg accctactgc
     1141 atttgttatt gttacagatg tacatgaggt tcttggagaa ggatttaaaa atttagagaa
     1201 ctaataaaaa ttaatatttt aagtatatat actattttaa ttaaaatacg atttattcaa
     1261 aaagagtgtc tcataaactt tttagttcat gagacactct tttttgtata aaatcaaatg
     1321 cattttcagt attttaacaa taaaaacagg attatctata ttttgaggca accactttat
     1381 tgaaatttta tttagaaaac aatcattact aaataaccag catagatatt aaatgattat
     1441 atctattgtt ttttcaattc atattgataa caaggaacat tttttacagt atttccagta
     1501 tgaatttttt tgatatactt tcctgtacat ttaaatccta ataaatctaa tgatttttta
     1561 gaattttggt tttcttcata ggttattgca attattttat ttaagtttag ttctctaaat
     1621 ccatactgta ttactctttt tgttatctca gtagcaaaat catgtcccca atagctcgga
     1681 tttatacata tacctatttc tgcttctaat gctttaggat ttatattatt aaaccccata
     1741 ttcccaataa ctttattgtc tcttttatat actatagcaa agtggccaat tttccctttt
     1801 ttatatggat ttagaaaaaa tgattttata aactttcttg tatcttctaa agatttatgc
     1861 tcactaaatg gtaaatgttt tactactatt ttatttttat aacagtcaaa aaaatcttca
     1921 acatcatcta atgatgcagg tcttagtata caacttctag tttctaaggt tttcatttga
     1981 ggatattcag aatcagacat ttctaatccc cctttataca ttaatctaaa tatactgttc
     2041 atatttaaaa tacgatatta attatataaa tgttctcaaa ttaaccttat ttttattttt
     2101 aataaaaact aattactata tgaatctttc aagtttacga atttatctct atttttatat
     2161 taaattactt gtgaaatatg tattctaaat tatatattat tagatatcat gcttaaaaat
     2221 tctttggttg ctaaggataa agatatatct tttaaatggc agtaacctat atttctctta
     2281 ggtattggct cttttatatc taatttaaac aatttctcat tttccaaata atctatgcta
     2341 aattcttcta taacacaaga cactccaaga tttatatacg caaattctaa aagtaattca
     2401 tgagaaccca attctatatc cgggtttagt tttatacctt ttgataaaaa atattcattt
     2461 acataaagtc ttgaatttgc tttattttct agcattatta aaggcaatgt gtttaattct
     2521 tccaatgata ttttcctacc tttgtattct atgtaatcat ttgcacatac aaatatatca
     2581 tgtatttcaa tacactcaac aatatttagt gtatcatctt ctattggcat gtttataatt
     2641 gctatatcta tattcccatc tttgagtaaa gatataagtt ctctactcgt tctatttatt
     2701 gtagatacat gtatatgtgg atacttctta tgaaaaattt ctaaatattt taacaagaaa
     2761 aaacgtgcag ctgtatctcc tactcctatt ttcagagaac cgtattctaa ttttttaaat
     2821 ttatttattc tatcttctcc tgatgatatt aactttaatg cagtagtgac atgttcactt
     2881 agaattttcc cctctggagt taattttact cccttattgt ttctataaaa taataaagta
     2941 tctaattgtt cttctaattg ctttattgac tggcttacag ctggctgtga cataaacaat
     3001 tccttagcag caagagaaaa actcttatta tttacaactg cattaaaaac cttataaagt
     3061 tcaagtttta tattcatata taattactcc ttatgtcaaa tattagtatt atttatttta
     3121 cttataatta tatttgaatt ataatataca tggtactaat atgacaatat taattcttta
     3181 ggaggatttt agataatgga tagattagtt ggaactgttt caagaggggt tagagcacct
     3241 attataagac aaggagatga cttagttaaa atagttgttg attctgtttt aaatgcgtca
     3301 aaaagtgaaa actttgaagt tagggacaaa gatgtaattg ccgttacaga agctgttgta
     3361 gctagagcac agggaaatta tgctcacgtt gacaatattg caaaagatgt aaaagataaa
     3421 tttggagatg atactgttgg agttatattc ccaattttaa gtagaaatcg ttttgctata
     3481 tgtttaaaag gaattgcaaa aggatgtaga aaagtcgtat taatgttaag ctacccttct
     3541 gatgaagttg gaaatcactt aataagtatt gatgaacttg atgataaggg aataaatcct
     3601 tggtcagatg ttttaacaga agaaaaatac agagaactat ttggatataa caagcataca
     3661 tttactggtg tagattatgt tgattattat aagaatttaa tcaaggattg tggtgcagag
     3721 gtagaaatag tatttgcaaa caatccaaaa actattctta actatacaac aagtatttta
     3781 acttgtgata tacatacaag acaaagaaca aagagaattt taagacaaaa tggagctaaa
     3841 aaagtatatt cacttgatga tataatgaca gcaagtgtag atggtagtgg ttacaatgac
     3901 cagtatggac ttttaggttc aaacaaagct acagaagaaa cagtaaaatt attccctata
     3961 aattgtgatg aagttgtaaa caaaatccaa ggtaatataa aagaaataac agggaaagat
     4021 gtagaagtaa tggtttatgg agatggagct tttaaagacc ctgtaggtaa aatatgggaa
     4081 ttagctgacc cagtagtatc tcctgcttat acaaaagggc tagagggaac tccaaacgaa
     4141 gtaaaattaa aatacttagc agataatgat tttgctgact tatctggtga tgagttaaaa
     4201 gaagcaattt caaaatacat agttgaaaaa gataataaat ctgatgattt aacaggaaac
     4261 atggtttctc aaggtactac tccaagaaga ttaacagacc ttataggttc tttagcagat
     4321 ttaacatctg gaagtggaga taagggtact cctattattt acatacaagg atatttcgat
     4381 aactatacaa aatagtatgt tttaataaga tttaaatttt ttggattata tatattaaca
     4441 tgaaaattta ttaaaacctc ttaatagata tgacttttca agagttatat cta
//
LOCUS       DAELLV010000265         4488 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.265, whole genome shotgun sequence.
ACCESSION   DAELLV010000265 DAELLV010000000
VERSION     DAELLV010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4488
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.265"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            231..1310
                     /locus_tag="KPD58_003296"
     CDS             231..1310
                     /locus_tag="KPD58_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBG0267025.1"
                     /translation="MPELLKNMYNRESLYEVAVAIQSVYNSFKVDEFIKSTMDETWNN
                     LELKARCRKISMSLGMYLPEDYKEALSILEKSVTGFYFAFFFPDFVEVYGQDDINWDL
                     SISALERNTEYWSSEFAVRAFIIKDEERMMAQMRKWSKHKSEHVRRLASEGCRPQLPW
                     GQAISKFKKDPTPVLPILEQLKTDTSTYVQKSVANNLNDISKTHPDLVISIAKDWYGK
                     NKSTNWIVKHGCRTLLKKGNRDVLALFGYDDTTSINIQDFTLETTSISIGEDLTFSFN
                     ILAKKATKTRLEYGIDYIKSNGKKSRKIFKISEVSLKENEKKSYMKKHSFADVSVRKH
                     YPGIHSIAIIINGIEKDKLDFELGI"
     gene            1634..2002
                     /locus_tag="KPD58_003297"
     CDS             1634..2002
                     /locus_tag="KPD58_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HBG0267026.1"
                     /translation="MDVYSFWNDTLAQDAVKIRKYFHENAYINWHDTNEHFTLEEFII
                     ANCEYPDKWNGKVERVLEFGNLVITVTHVYAINKPLSFHVVSFISLKDDKIISIDEYW
                     GEDDDVPQWRLDKHIGKPIK"
     gene            2119..2922
                     /locus_tag="KPD58_003298"
     CDS             2119..2922
                     /locus_tag="KPD58_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0267027.1"
                     /translation="MEEKYILSDRGKVYYWIKESNSVFAKNLIFLPGLTANHHLFDFQ
                     IQYFSKNHNVLVWDAPAHGKSRPYLDFSYSNLVEELKVILDKQNMKKVVLIGQSAGGF
                     VAQSFIVKYPNMVEGLMMIGTCPYGTDYYSKSDIFWLKQTEWMLKLFPDKFLRNIISK
                     MCGATSEGRRSMLKMLEGYEKKELCHLMYLGFAGFIPEIQDLNIHCPVCLVVGDKDKT
                     GKVRKYNEQWHNKTGYPLHIIKNASHNVNVDKPNELNQIMDIFIKQLNI"
     gene            3307..4197
                     /locus_tag="KPD58_003299"
     CDS             3307..4197
                     /locus_tag="KPD58_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG0267028.1"
                     /translation="MIESIPAKQILQKVKFDNTRWFGIDYNMNLYRGCSHGCIYCDSR
                     STIYNIENFDKVRYKENVIEILSKELRSKRKKGVVGIGAMSDTYNPFEKQLCITKQAL
                     DLISENHFGVSIDTKSSLVVRDIPILQKIKKNNSAIVKLTITTANDELSKKIEPYVNP
                     SSVRFEAVKELNDSGIFCGILLTPMLPFLTDTKDEIRAIVEKAHKANAKFIYCMYGVT
                     MRSGQREFFYEHLRDISPKLVLKYQKTYGLNYVCTVQNKDSCEKLLREECARYGILTD
                     MKEIIKAYKRSESYTQIKFF"
BASE COUNT         1709 a          480 c          844 g         1455 t
ORIGIN      
        1 cagtaaagta taaaattcta ttactataat aaaagataga actagaagtc tttatgaaag
       61 tgggaaaagt gtttatataa ataaatttat atagtttata gtttaacagt aggaaatatc
      121 gtatgagtga gaatatcgta ttttacataa agatttagca atatgacaca ctgttgtcgt
      181 gttctgtagg atatgattaa taaatcagat atgaaaggag agtgagtaat atgccagaat
      241 tactaaaaaa tatgtataat cgtgagtcac tttatgaagt tgcagtagct atccaatcag
      301 tttacaactc atttaaggta gacgagttca taaaatctac aatggatgag acatggaata
      361 acttagaatt aaaagccaga tgtcgaaaga taagtatgag tttaggaatg tatttaccag
      421 aagactataa ggaagctctt tccattcttg aaaaatcagt tacaggtttt tattttgctt
      481 ttttctttcc agattttgtt gaagtttatg gtcaggacga cataaattgg gatttatcta
      541 taagtgcatt agaaagaaat actgagtatt ggtcttcaga atttgctgta agagcattta
      601 tcatcaagga tgaagagcgt atgatggcac agatgcgtaa atggtctaag cataagagcg
      661 aacatgtacg aagacttgca agtgaaggtt gtcgtcccca gttgccttgg ggacaagcaa
      721 tatccaaatt taaaaaagac cctacacctg tactgcctat attggagcaa cttaaaacag
      781 atacatcaac gtatgtacaa aagagtgttg ctaataatct aaatgatatc tcaaaaacac
      841 atccagattt agtcataagc attgcaaagg attggtatgg taaaaataaa tccactaact
      901 ggattgtaaa gcatggttgt agaactctgc taaaaaaagg aaatcgtgat gtattagctc
      961 tttttggata tgatgatact acttcaataa atattcagga ttttacttta gaaacaacgt
     1021 ctatttctat tggagaggat ttaacttttt catttaatat tttagcaaaa aaagcgacaa
     1081 aaacaaggct cgagtacgga attgattata ttaaatctaa tggaaaaaag agtaggaaga
     1141 tatttaaaat atcggaggtt tcattaaaag aaaatgagaa aaagtcatat atgaaaaaac
     1201 attcatttgc agatgtaagt gtaaggaaac attatccagg tatacactcg attgctatta
     1261 ttattaatgg tatagaaaaa gataaacttg attttgaatt aggtatataa ttttactaac
     1321 agaataatct atatcagata ctttgctaaa cttatataat atattttact aaatttatat
     1381 taggtagttt actacttgtt tactgaattt aaatacaaag aaatccaggt gttaatccct
     1441 gccgattaaa ggaatatgag cataacacaa aagtagaact tatcaattct gttacgggta
     1501 tagtagtaaa tccatagaat cacaattaga agatgaagtg taaaaaataa gttagtgttg
     1561 attttagttg ctagtgatat tgaatagtta tgcaataccc atataaatta gaatttgtag
     1621 aggagaaatg taaatggatg tatatagttt ttggaatgac actttggcac aagatgctgt
     1681 taagataaga aaatattttc atgaaaatgc ttatattaat tggcatgata caaatgagca
     1741 ttttacttta gaagaattta ttattgcaaa ttgtgagtat cctgataaat ggaatggtaa
     1801 agtagaaagg gtgctagaat ttggaaattt agtaattaca gtgacacatg tttatgcaat
     1861 aaataaacca ctatcatttc atgtcgtttc atttatcagc cttaaagatg ataagattat
     1921 ttctattgat gaatattggg gagaggatga tgatgtgcca cagtggagat tagataaaca
     1981 tattggaaag ccaataaaat agtgtttgtt ctgttttata actgaaattt tgataacatt
     2041 tgatattttg atttgtcatt ggtcaacata gtcgttagtg taataattta aaaaagtaga
     2101 atttggagag tgaacactat ggaagaaaaa tacatcttat ctgaccgagg gaaagtatat
     2161 tattggataa aagaaagtaa tagtgtgttt gcaaagaact taattttttt gccaggacta
     2221 acagcaaatc atcatctttt tgatttccaa atacagtact tttcaaaaaa tcataatgta
     2281 ttggtatggg acgctcctgc tcatggaaaa tcaagacctt acttagattt ctcatattct
     2341 aacttggtag aagaattgaa agtaatttta gataaacaaa atatgaaaaa ggttgtactt
     2401 atagggcaat cggcaggggg atttgtggca cagtccttta ttgtaaaata tccaaatatg
     2461 gtagaaggat taatgatgat tggaacttgt ccatatggaa ctgactatta tagtaagtca
     2521 gatattttct ggttaaaaca gactgaatgg atgttaaagt tattcccaga taaatttttg
     2581 cgaaacataa tatctaagat gtgtggagct acgtctgaag gcagaaggag tatgctgaaa
     2641 atgttagaag gctacgaaaa aaaagaactg tgtcatctca tgtatctggg atttgctggg
     2701 ttcataccag aaattcaaga cttaaatata cattgtccag tttgtttggt ggttggagat
     2761 aaagacaaga cgggtaaagt gagaaaatat aatgagcagt ggcacaataa gactgggtat
     2821 ccattgcaca ttataaaaaa tgcttcacat aatgtaaatg tcgataaacc taatgaacta
     2881 aatcagatta tggatatatt tattaagcaa ttaaatatat aaaaactaca tactcattag
     2941 gtaaattatg tagtatatgt gtttacagat ggttatagaa ataaatatgt aataaactac
     3001 ttaacactta aaaagtggat ttgtccagag cataatacaa agcatgatat ataaattttt
     3061 aagcgaaata catcaaaaga agtattagaa ttagtggaga taaaacagct attagagatt
     3121 ataaataaat agttttaaag gtactacgaa tatagaggaa ggtaaatcat acgttattca
     3181 aggattataa atgattagtt taaaagtact atcggatagg tctctgcata tttaaacctt
     3241 agtaaaggta gttaaagtag tagtttaaaa actaaaaaat tatataaagg aattaggtat
     3301 aagaaaatga ttgagagtat accagcaaaa caaattttac aaaaagtgaa atttgacaat
     3361 acaagatggt ttggaattga ctataatatg aatctctatc gtggttgttc tcatggatgt
     3421 atatattgtg atagccgtag cacaatatac aacatagaga attttgacaa agttagatat
     3481 aaagaaaatg tgatagaaat tttaagtaaa gaattgcgtt caaaaagaaa aaaaggtgta
     3541 gtaggcattg gagctatgtc tgacacatat aatccttttg aaaagcaact ttgtattaca
     3601 aagcaagcac tagatttgat ttcggaaaat cattttggag tgtctattga tactaaaagt
     3661 agtttggtgg ttagagatat accgattttg cagaaaatta aaaaaaataa ttctgctatt
     3721 gtaaagttga ctataaccac tgcaaatgat gaattatcaa agaaaataga accttatgta
     3781 aatccaagtt ctgtacgatt tgaggcagtg aaagaactta atgattctgg aattttttgt
     3841 ggtatattat taactcctat gcttccattt ttgactgata ctaaagatga aatacgagca
     3901 attgtagaaa aagcacacaa ggctaatgca aaatttattt attgcatgta tggtgtaaca
     3961 atgcgtagtg gtcagagaga gtttttttat gagcatttaa gagatatatc accaaaattg
     4021 gtacttaaat atcagaaaac atatggttta aactatgtat gtactgtaca aaataaagat
     4081 tcttgtgaga agttgttaag agaagaatgt gcacgttatg gtattttgac ggatatgaaa
     4141 gagattataa aagcgtacaa gaggtcggag tcatatactc aaataaaatt tttttaagat
     4201 aggctttgga tgagatataa tagtggtgat tgatgtgttg atattatagt tgattgttag
     4261 agaggatatt atagatgaat attagtattg atattctagg taattgctag agttagtatt
     4321 atagatgaat attaatattg atattctagg taattgctag agttagtatt atagatgaat
     4381 attagtattg atattctagg taattgctag agctaatatt atagataaat attagtattg
     4441 atattctagg taattgctag agctgatatt atagatgaat attagtat
//
LOCUS       DAELLV010000266         4484 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.266, whole genome shotgun sequence.
ACCESSION   DAELLV010000266 DAELLV010000000
VERSION     DAELLV010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.266"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            143..1141
                     /locus_tag="KPD58_003300"
     CDS             143..1141
                     /locus_tag="KPD58_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HBG0267029.1"
                     /translation="MKILVFGARDYEEPVIKKWSEEHKDVQVDIYPENMTEENVVKAK
                     GYDGISIQQTNYIDNPYIYETLKDAGVKVIASRTAGVDMIHFDLVNENGLIVTNVPSY
                     SPNAIAELAVTQAMNLLRKTPLVKKKVCEGDYRWIAELLGTEVRSITVGVIGTGKIGA
                     TSAKLFKGLGANVIAFDQYPNSDLNDILTYKDSLEDLLKEADLITLHTPLLEGTKHMI
                     NKDTLAIMKDGAYIVNTGRGGLINTGDLIEALESGKIRAAALDTFETEGLFLNKKMNP
                     GELTDPEINKLLSMEQVIFTHHLGFFTSTAIENIVYSSLSSAVEVIKTGTATNRVN"
     gene            1503..2030
                     /locus_tag="KPD58_003301"
     CDS             1503..2030
                     /locus_tag="KPD58_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04002 family protein"
                     /protein_id="HBG0267030.1"
                     /translation="MEKMNKVTSKKTTYIVTSALFASIICLTIAYILHIPVGGNNGYV
                     HIGDAFIYLAATILPTNYAIAASAIGAGLADLSTGAAIWVIPTIIIKPILVLFFTSKS
                     DKIINKRNIVASVVAGIVGLVLYMFAEGIIIGSFTSAFVMSLLGLLQPIGSFIVFIIL
                     GMALDKLDFKKRYFN"
     gene            2057..2653
                     /locus_tag="KPD58_003302"
     CDS             2057..2653
                     /locus_tag="KPD58_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04100 family radical SAM protein"
                     /protein_id="HBG0267031.1"
                     /translation="MNILYTIENSIYVNITNTCPCSCVFCIRNEKDEVANSGSLWLEH
                     EPSVDEVKEAFNKYNLDDYDEIVFCGYGEPLMRINELIEIAKFIKEKSSIKIRINTNG
                     LSDLIHNKKTAILLKNVIDAVSISLNAPNKEAYNRVTQPKFGEKSFDYMLDFARDCKK
                     YIKEVAFSVVDEISPEEIEESKQLAKKLDIPLRVRHKN"
     gene            complement(2718..4301)
                     /gene="asnB"
                     /locus_tag="KPD58_003303"
     CDS             complement(2718..4301)
                     /gene="asnB"
                     /locus_tag="KPD58_003303"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBG0267032.1"
                     /translation="MINLEEKYLTRKDTYMIKILEYNGHIRNWKVLCEELGIDSSLCR
                     DERERAILVEAYKTWGYDMANHMHGMFAFALWDTVEKKLFCLRDQFGVKPFYYYETEN
                     GKLLYGSSIRKIMEQPGFVKELNEDMLQIYMSFTYVAGENTFFRGVKKLMPGHYLVYQ
                     NKTVCIGRYWKPEFHPDHSKSIEEWTDEIHTTLQKIMPEVKSENETAELFLSGGVDSS
                     YILAMSDIEKTGSCGYEEERFDESKLAQQTANILGCKNSRYIITPEEYFASVPYVMYH
                     MEQPTGDASAIVFAIGCKAAAENTNICYSGEGADEFFGGYNIYHKAECYGENLKTFYI
                     GNTNIMNEGEKRKILKKYNPDVLPIEVVKGIYEETEELDTLTNMSNVDIQIWLEGDIY
                     FNIDKMSTAVGLEVRMPLTDTRIFDIASRIPSEYKVKGKENKIAFRTAASKTLPEEIA
                     FRNKLGFIVPIRIWMADDRYNKDIRDKFNSEIADKFFNIDEINEIFNDYISGNSDNWR
                     KIWMIYTFLVWYEIYFVKC"
BASE COUNT         1580 a          696 c          649 g         1559 t
ORIGIN      
        1 caaaataaca cagggaaaac aatttcaaaa ctaaattttt aaccttaaaa attttgtata
       61 aaaaatataa ccttatatgc tacatattat ttctaaatga aaaattacca taataattta
      121 ataattacag gagggtattg atatgaaaat actagtattt ggagcacgcg attatgaaga
      181 accagtaata aaaaaatggt ctgaagaaca taaggatgtt caagtggata tttatcctga
      241 aaacatgact gaagaaaatg tagttaaagc taaagggtat gatggtatat ctatacaaca
      301 aactaactat atagataatc cttatattta tgaaacttta aaagatgctg gggttaaagt
      361 tatagcttca agaactgcag gggttgacat gatacatttt gatttagtta atgaaaatgg
      421 acttatcgtt acaaacgttc cttcttattc acctaatgca atagctgaat tagctgttac
      481 tcaagctatg aaccttttaa gaaagactcc tctagtaaag aaaaaagtct gtgaaggtga
      541 ttaccgttgg atagctgaac ttcttggaac agaagttaga tctattacag ttggtgttat
      601 aggtacagga aaaataggtg ctacttctgc aaaattattc aaaggcctag gagctaatgt
      661 aattgcattt gaccaatatc caaatagtga tttaaatgat atattaactt acaaagattc
      721 tttagaagac cttctaaaag aagctgacct tataacatta catactcctt tacttgaagg
      781 aacaaaacat atgataaata aagatactct agctataatg aaggatggag cttacatagt
      841 aaatactggc cgtggtggtt taattaatac aggggattta atagaagcac tagagtcagg
      901 aaaaattaga gctgctgccc ttgatacatt tgaaactgaa ggattgttct taaacaaaaa
      961 aatgaatcct ggagaattaa ctgacccaga aataaataaa cttctttcta tggaacaagt
     1021 tatattcact catcaccttg gtttcttcac tagtacagcg attgaaaata tagtttattc
     1081 tagtttaagt agtgctgtag aagttataaa aacaggaact gctactaata gagtaaatta
     1141 gtattttata aatatatttt ttcattaatt agctattatc taacaaaata gcctttaatt
     1201 tcccaaatat aagagagtca gctcagtata tattgagtag actctcttat attatttttt
     1261 atattaaact tgtactcttc ttattcatac caaattaaat ttatattaac ttattctaac
     1321 tactcctact ctatacattt tacctatttt tacttttcta attatataca tttgaatggg
     1381 tacaatctgt attttacaaa aaaaacacct aacaaactat gaaaattgta tgattaatat
     1441 taatatattt ataagaaaac ataacaatta tgttagctct ggatttttag ggagggagta
     1501 tattggaaaa aatgaataaa gttacttcta aaaaaactac ctatattgtt acttcagcat
     1561 tatttgcatc cataatatgc ctgacaatag catatatatt acatattcca gttggtggaa
     1621 acaatggata tgttcatata ggagatgctt ttatttatct agctgcaact attttgccaa
     1681 caaactatgc tatagctgct tctgcaattg gtgctggtct agctgattta tctacaggtg
     1741 ctgctatctg ggtaatacca actataatca taaaaccaat acttgtatta tttttcactt
     1801 ctaaaagtga caagataata aataagagaa atatagttgc ttcggttgta gcaggtatag
     1861 taggattagt tttatacatg tttgctgaag gtataattat tggaagtttt acaagtgcat
     1921 ttgtaatgag cttacttggc ttattacagc caataggaag ttttattgtt tttataatct
     1981 taggtatggc acttgataaa cttgatttta aaaaacgtta ttttaattaa gaatttaaat
     2041 ttatggaggg aaaattatga atatcttata tacaattgaa aatagtatct atgtaaatat
     2101 aactaataca tgtccatgta gttgtgtttt ttgtataaga aacgaaaaag atgaagttgc
     2161 aaatagtggt agtttatggt tggagcatga gccaagtgta gatgaagtaa aagaagcctt
     2221 caataaatac aatttagacg actatgatga aatcgtattt tgtggatatg gcgaaccatt
     2281 aatgagaata aatgaattga tagagatagc aaagtttata aaagaaaaaa gtagcataaa
     2341 gataagaata aatacaaatg gtctaagtga tttaatacat aacaaaaaaa cagctattct
     2401 gttaaaaaat gttatagatg cagtatcaat aagtcttaat gccccaaata aagaagctta
     2461 taatagagtt actcaaccaa agtttggaga aaaatcattt gattatatgt tagattttgc
     2521 aagagattgc aaaaaatata taaaagaagt agctttttca gtagtagatg aaatatctcc
     2581 agaagaaata gaagaatcta aacaactggc aaaaaaactt gatataccat taagagttag
     2641 acataagaat tagcaagtaa aattataaaa cattaagaat cacctgaatg gtatttcagg
     2701 tgattcttcc tcttgtatta acattttaca aaatagattt cataccacac taggaatgtg
     2761 taaatcatcc aaatctttct ccaattatca gagtttccac taatgtagtc attaaaaatc
     2821 tcattaatct catctatgtt aaagaattta tctgctatct cactattgaa tttatctcgg
     2881 atatccttat tatatcggtc atcagccatc catatacgaa ttggcacaat aaatcctaat
     2941 ttatttctaa aagcaatctc ttctggtaat gtttttgatg cagctgtacg aaaggcaatt
     3001 ttattctctt ttcctttcac tttatattct gatggtattc tggatgcaat atcaaatatt
     3061 cttgtatctg taagaggcat tctgacttcc aatcctactg cagtactcat tttatctata
     3121 ttaaaataaa tatctccttc caaccatatt tgaatatcta catttgacat gttagtaagt
     3181 gtatctagtt cttctgtttc ttcataaatt ccttttacca cttcaatagg aagcacatca
     3241 ggattgtatt tcttaagaat ttttcttttc tcaccctcat tcataatgtt tgtatttcca
     3301 atataaaatg tcttcaaatt ctctccataa cactctgcct tatggtatat attatatcca
     3361 ccaaaaaact catctgctcc ttctccagag taacagatat ttgtgttttc agctgctgcc
     3421 ttgcatccaa tagcaaaaac gatagcagat gcatctccag ttggttgctc catatggtac
     3481 attacataag gtactgacgc aaaatactct tcaggtgtaa ttatatatct cgaattttta
     3541 cacccaagaa tattagctgt ctgttgtgct aattttgatt catcaaaacg ctcttcctca
     3601 taaccacatg aacctgtttt ttctatatct gacattgcca atatataaga tgaatcaaca
     3661 cctcctgata ggaataactc tgcagtttca ttctctgatt ttacctctgg cataattttc
     3721 tgaagtgttg tatgaatttc atccgtccat tcctcaatag acttgctgtg gtcaggatga
     3781 aattcaggtt tccaataacg accaatacat actgttttat tctgatagac aaggtaatgt
     3841 cctggcataa gcttctttac tccacggaag aatgtatttt cacctgctac atatgtgaaa
     3901 gacatataaa tctgtaacat gtcttcattt aactctttca caaatcctgg ttgctccata
     3961 atcttacgaa tagatgaacc atagagaagc ttcccatttt cagtctcata ataatagaaa
     4021 ggttttaccc caaactggtc tcttaggcag aagagtttct tttccacagt atcccataat
     4081 gcaaaagcaa acattccatg catgtgattt gccatatcat atccccatgt cttatatgct
     4141 tccactaaaa ttgctcgttc acgctcatct ctacataatg aagaatcgat tccaagttct
     4201 tcacaaagaa ctttccagtt tcgaatatgc ccattgtatt ctagaatttt tatcatatat
     4261 gtgtcttttc gtgttaaata cttttcttct aaatttatca tatgtgtatc ctttcgtatt
     4321 aaaacattta acttctaaat ttattaaatg tttatatata tttattcact tataatcact
     4381 gaaaaatcga cttttttcaa agtaaattct ttttgaatta gtactaatct gctctttatt
     4441 aatggcataa atatgattat caagtattat cagtgttata acat
//
LOCUS       DAELLV010000267         4384 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.267, whole genome shotgun sequence.
ACCESSION   DAELLV010000267 DAELLV010000000
VERSION     DAELLV010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4384
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.267"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            220..1818
                     /locus_tag="KPD58_003304"
     CDS             220..1818
                     /locus_tag="KPD58_003304"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HBG0267033.1"
                     /translation="MSLLVFGHKNPDTDSICSAISLAHLKNKLGIKATAYALGDIRKE
                     AKYALDYFKVDAPEILDNVRIQVRDLNYDKVVPLTPTSSILEAYNLMDEKNVKTLPVT
                     YDDGTFAGIITMKEIAKNLLHQHFTTIHTSLSNICKNLNGTILVDCGEDIKGRVVTLS
                     FGMETLIETLKEGDIAIVGDRYDSIEYAIDTKVKLLILTNHTEISKDLLALAKINGVS
                     VVSVESDNYKTSNVINQCSFLDSIEATENLITFKEDDYMDEIKEIMLETNFRSYPILD
                     DDNKFLGLVSKGHLLNPSKKNVVLVDHNEYAQSADGIEQANIVEIIDHHKLGGISTDV
                     PMSFRVMPVGCNSTIIYQMYKENNVEIPYEIAGLLLSAILSDTLLFKSPTTTDIDKKA
                     CEELSKIAKVDMEKYAMDMFKCGTSLDEYTIEEIVNMDFKEFNMSGHRVGIGQVFTLD
                     IDSIFAKKDEFLSYINSTDYDKLILAVTDIIKEGSYLIYKAEDSLIANAFGVEGVQGT
                     FAPGVVSRKKQLVPNLTTAIKNFK"
     gene            complement(2057..4102)
                     /gene="bclA1"
                     /locus_tag="KPD58_003305"
     CDS             complement(2057..4102)
                     /gene="bclA1"
                     /locus_tag="KPD58_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA1"
                     /protein_id="HBG0267034.1"
                     /translation="MRNIILYLNDDTFISKKYPDKNFSNLDYCLIGSKCSNSFVKEKL
                     ITFFKVRIPDILKDKSILKAELFIHIDSNKNHIFKEKVDIEIKRISEYYNLRTITWND
                     RVSMENIRGYLPVGISDTSNYICLNITGTIKAWAMNKYPNYGLALSLNYPYQILEFTS
                     SRGCNKPYILVTFEDRIIDNCYPKCECLPIRITGPMGPRGATGSTGPTGVTGPTGSTG
                     ATGSIGPTGPTGNTGATGSIGPTGVTGPTGSTGATGSIGPTGVTGPTGNTGVTGSIGP
                     TGATGPTGNTGVTGSIGPTGVTGPTGNTGEIGPTGATGPTGVTGSIGPTGATGPTGEI
                     GPTGATGATGVTGSIGPTGATGPTGATGVTGEIGPTGEIGPTGATGPTGVTGSIGPTG
                     ATGEIGPTGEIGPTGATGPTGVTGSIGPTGATGPTGATGEIGPTGATGPTGNTGVTGE
                     IGPTGATGPTGNTGVTGEIGPTGATGPTGVTGEIGPTGNTGATGSIGPTGVTGPTGAT
                     GSIGPTGATGATGVTGPTGPTGATGNSSQPVANFLVNAPSPQTLNNGDAITGWQTIIG
                     NSSSITVDANGTFTVQENGVYYISVSVALQPGSSSINQYSFAILFPILGGKDLAGLTT
                     EPGGGGVLSGYFAGFLFGGTTFTINNFSSTTVGIRNGQSAGTAATLTIFRIADTVMT"
BASE COUNT         1299 a          780 c          665 g         1640 t
ORIGIN      
        1 tttattttaa ataaaagtaa atttaattcg atatttgatt gggtaaaagt atatataata
       61 ataattttta tattaaattt gtagtattta tgtaagaaat aaattttata taatattttt
      121 tcactaaatt tttttctttt tacaacaaat actcactacc ttttgatata atatttttat
      181 caattattat tatattcaag ttttgaaagg agtattaaaa tgagtttatt agttttcgga
      241 cataaaaatc cagatacaga ttcaatatgt tctgcaattt cattagcaca tttaaagaac
      301 aaactaggaa taaaagcaac agcttatgct ttaggagata tcagaaaaga agctaaatat
      361 gccttggatt actttaaggt agatgcacct gaaattttag ataatgttag aatacaagtt
      421 agagatttaa attatgataa agtagtacct ttaacaccaa cttcatctat attagaggct
      481 tataacctta tggatgaaaa aaatgttaaa acactacctg taacttatga tgatggaact
      541 tttgctggta tcattactat gaaagaaata gctaaaaatc tacttcatca acactttaca
      601 actatacaca catcactatc aaacatttgt aaaaacctta atggtactat tcttgtggac
      661 tgtggtgaag atataaaagg tagagtagtc actttatcat ttggaatgga gactctaata
      721 gagactttaa aagaaggaga catagctatt gttggagata gatatgatag tattgaatat
      781 gctattgata caaaagttaa acttttaata cttactaatc atacagagat ttcaaaagat
      841 ttgctagctc ttgctaagat aaatggagtg tctgttgttt ctgttgaaag tgacaattat
      901 aaaacttcta atgtaataaa tcaatgtagt ttcttagact ctatagaagc tacagaaaat
      961 ttaattacat tcaaagaaga tgattatatg gatgaaataa aagagattat gctagaaaca
     1021 aacttcagat cttatccaat attagatgat gataataagt ttttaggttt agtttcaaaa
     1081 ggtcatctat taaacccatc taaaaagaat gttgttttag ttgaccataa cgaatatgct
     1141 caaagtgctg atggaattga acaagcaaac atagttgaaa taattgacca tcataaactt
     1201 ggtggtattt ctactgatgt tcctatgtca tttagagtta tgcctgttgg atgcaatagt
     1261 actataattt atcaaatgta caaagaaaat aatgttgaga taccttatga aatagccggg
     1321 cttcttttat ctgctatatt atcagataca ttattattta agtctccaac aacaactgat
     1381 atagataaga aagcttgtga agaattaagt aaaatagcaa aagtagacat ggaaaaatac
     1441 gctatggata tgtttaaatg cggtacttct ttagatgaat atactataga agaaatagta
     1501 aatatggact ttaaagaatt taatatgagt ggacatagag ttggtattgg acaagtattt
     1561 accttagaca tagactctat atttgctaaa aaagatgagt ttttatctta tataaattcg
     1621 actgattatg acaaactaat tcttgcggtt acagatataa tcaaagaagg ttcttattta
     1681 atctataaag cagaagacag cttaattgct aacgcatttg gtgttgaagg tgttcaaggt
     1741 acatttgcac caggtgttgt atcaagaaaa aaacaattag tccctaattt aactactgca
     1801 atcaaaaatt ttaaataata taaaattttt aaataacgaa aactgtctca aatctatatt
     1861 tgagacagtt ttttattata tatatatata ttgataataa agttaaataa aaatccataa
     1921 atttaaaatt tttatgaata tattttatta ttattgttat tttattcatt ctaaaatagg
     1981 taaaactcaa tatataaaaa tacttttgtc atttaaataa agcctaaata gtttttaaga
     2041 ttattttaga cacgttttaa gtcataacag tatcagctat tctaaatatc gtcaaagtag
     2101 ccgcagttcc tgctgattgc ccatttcgta tccctactgt tgtagatgaa aaattattta
     2161 ttgtaaaagt agtcccacca aataaaaaac cagcaaaata tccagaaagt actcctccgc
     2221 ctcctggctc agtagtaagc cctgccaaat cttttcctcc taaaattggg aataggatag
     2281 cgaaagaata ttgatttata cttgatgaac ctggttgtaa tgctactgaa actgatatat
     2341 aatacacacc attttcttgt actgtaaacg taccatttgc atctactgtt atacttgaac
     2401 tatttcctat tattgtttgc caacctgtta tagcatctcc attatttagt gtttgtggag
     2461 atggtgcatt tacgaggaag ttagcaactg gctgagagga attgcctgtt gctcctgttg
     2521 gacctgttgg gcctgttact cctgtcgctc ctgttgctcc cgttggtcct atacttcctg
     2581 tcgctcctgt tggacctgtt actcccgttg gccctatact tcctgtcgct cctgtatttc
     2641 ctgttggccc tatttctcct gtcactcctg ttgggcctgt tgctcccgtt ggtcctattt
     2701 ctcctgtcac tcctgtattt cctgtcggac ccgttgctcc cgttggtcct atttctcctg
     2761 ttactcctgt atttcctgtt ggacctgttg ctcccgttgg tcctatttct cctgtcgctc
     2821 ctgttgggcc tgttgctccc gttggtccta tacttcctgt cactcctgtt gggcctgttg
     2881 ctcctgttgg tcctatttct cctgttggtc ctatttctcc tgtcgctccc gttggtccta
     2941 tacttcctgt cactcctgtt gggcctgttg ctcccgttgg tcctatttct cctgttggcc
     3001 ctatttctcc tgtcactcct gtcgctcctg ttggacctgt tgctcctgtt ggtcctatac
     3061 ttcctgtcac tcctgtcgct cctgttgctc ccgttggtcc tatttctcct gttggtcctg
     3121 ttgctcctgt tggtcctata cttcctgtca ctcctgttgg acctgttgct cccgttggtc
     3181 ctatttctcc tgtatttcct gttgggcctg ttactcctgt tggtcctata cttcctgtca
     3241 ctcccgtatt tcctgtcggg cctgttgctc ccgttggtcc tatacttcct gtcactcccg
     3301 tatttcctgt cggacctgtt actcctgttg gtcctatact tcctgtcgct cctgtacttc
     3361 cggttgggcc tgttactccc gttggcccta tacttcctgt tgctcctgta tttccggttg
     3421 ggcctgttgg tcctatgctt cctgtcgctc ctgtacttcc ggttgggcct gttactcccg
     3481 ttggtcctgt acttcctgtc gctcctcttg gtcccattgg acctgtaatt ctaattggaa
     3541 gacactcaca tttaggataa caattatcta taattctatc ttcaaatgtt acaagtatat
     3601 acggtttgtt acaacctcta ctagatgtaa attcaagaat ctgataaggg taatttaaag
     3661 ataaagctaa cccataatta ggatatttat tcattgccca tgcttttata gttcccgtaa
     3721 tatttaaaca aatatagttg gatgtatcac ttatcccaac tggtaaatat cccctaatat
     3781 tttccataga cactctatca ttccatgtta tagttcgtaa attataatat tcacttattc
     3841 ttttaatttc aatatctact ttttctttaa aaatatgatt cttatttgaa tcaatatgaa
     3901 taaataactc tgcttttaat atacttttgt cttttaatat atctggtatt ctcactttaa
     3961 aaaaagtaat caacttttct tttacaaaac tatttgaaca tttacttcct attaagcaat
     4021 aatctaaatt actaaagttt ttatctggat attttttaga tataaaagta tcatcattta
     4081 aataaagtat aatatttctc atttataatc cctccatttt gggtctatat catactatgt
     4141 ttaaaataca tgcttagtta caatataatt aaaatttata gtattaaatt tttatataat
     4201 aatgctgatt tctacaaaaa aagaacatgt cattagaaat ttctaatagc atgttctttt
     4261 tcattattat atattacact ttaaattatt ccttgactgg cttagctggt gtaaactaaa
     4321 ccagtctttc taaaatatta agcacttttt tcttattatg ttttttagga tttattgtct
     4381 atat
//
LOCUS       DAELLV010000268         4283 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.268, whole genome shotgun sequence.
ACCESSION   DAELLV010000268 DAELLV010000000
VERSION     DAELLV010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4283
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.268"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            36..1040
                     /locus_tag="KPD58_003306"
     CDS             36..1040
                     /locus_tag="KPD58_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG0267035.1"
                     /translation="MRFKRILILMLTVAMIAGMLVGCANNPSNNPSSSKDNKSDISDK
                     SNSKKSVSMILDIEGTNNEAMNNSALLALNNAQKKLNIDTNKVESDDSSTFSNSIDIL
                     CNDNYDLIIAVGARFAKPLEMVAKKYPKQQFAIIDYEYDKQPSNITSISYEDNKSGYL
                     AGLIAGKMTESDKVGFIGGTKSSSRDKFESGFREGVKFSNSSIRDISVEYADVFKDSK
                     SVESIAKKMMDNGVDIIFSTTEDDSKAVIDAVRAKNKKVIATNKDQHELAPENVISSV
                     VKDFENPTYNLIQSFVKGNYKGGKVIENTVKSGSTGLAQNSSQNIPPDVLEYVNKNNK
                     "
     gene            1166..2278
                     /locus_tag="KPD58_003307"
     CDS             1166..2278
                     /locus_tag="KPD58_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG0267036.1"
                     /translation="MKIKRILMACFVVVFTIFILAICNFDFGFDKVNKNNSNIDSAKS
                     KQNESKSLPTSAEKDKSNSKSKNKEDDTEDDSYVRKTIGVLLDTENLNNESYNNNVLS
                     ALKRADEDFDVRIKLEKPKTSKSFKESIEKLHKYEPDLIIAVGARFAEPIKEAATKYP
                     KQQFAIVDYTYKVQPANVISVSFQDNVTGYLAGLIAGKMTDTGKVGFIGGVEGSSRDK
                     YESGFRKGLKTSNKDAELLVKYSNIFENPESARTIAKEMRDSDRDVVFSATEDDSKRA
                     IQVARENGGKVIAINKDQYDMAPDNVISSIIKDVEQPVYELIKNVAKDGFKGSKVMEF
                     KIKDGELGLTSKSSRNIPPDVLEYIKKEYNKVKDTY"
     gene            2634..3395
                     /locus_tag="KPD58_003308"
     CDS             2634..3395
                     /locus_tag="KPD58_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0267037.1"
                     /translation="MEFTREPFILDDDLCLALVDKRISYLMENELKLRNIEIIKTIEC
                     KELYESIKYHPDICICNLGKGDIIVAPNVYNQYKELLSRYKFNIIKGEANLLNRYPYD
                     IPYNIAIVGDYAIHNFKYTDKAILDYIENNKLKKINIEQGYSKCSICIVDRTSIITSD
                     KGIWKSMKNTEIECLLIEKGHINLFEMNYGFIGGCTGLISKDKLAFCGDVKKHPDYER
                     IKSFVESKNKEIVTLSCENLLDIGSIVPLMTRKER"
     gene            3398..4216
                     /locus_tag="KPD58_003309"
     CDS             3398..4216
                     /locus_tag="KPD58_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HBG0267038.1"
                     /translation="MVEKDNISSMKFLRNNNVNDRLIYTNDIVEVVIDSAKQEVLNKK
                     ILNGTKTEQRALNIDEISTEITSMIISIMKEKLLKEYILKCYGRTYPEDKNNIYIYSL
                     NIFAKKEIFMRETVFTRVRNYIEENDFINVEGFIRFRMREFMKYISAIGDIAVEEYLI
                     KKDQDEFIRVLKYFIDTQEEKIDLLKVHIMEDNTFVLYDKNGNKIDSIDDEEIINMVI
                     RENLNYEDFLISTLLTLCPKKIEILDLLNNNCSKEIIDTVEAIFENKVSIIMEN"
BASE COUNT         1791 a          417 c          770 g         1305 t
ORIGIN      
        1 taaaaatatc gtaaatgtat gaggagtgaa atttaatgcg tttcaaaaga atattgatat
       61 tgatgttaac tgtagcaatg atagctggaa tgttggttgg gtgtgctaat aacccaagca
      121 ataatcctag ctctagcaaa gataataaaa gtgatataag cgataaaagt aattcaaaaa
      181 aatctgtatc aatgatacta gatattgaag gaacaaataa tgaagctatg aataattcag
      241 ctttattagc attaaacaat gctcaaaaaa aacttaatat agatacaaac aaagtggaat
      301 cagatgattc ctcaactttt tctaatagta tagatatttt atgtaatgac aattacgatt
      361 taattatagc tgtaggagct agatttgcaa aaccattgga gatggtagcc aagaaatatc
      421 caaagcaaca atttgcaatt atagactatg agtatgacaa gcaaccttct aatataactt
      481 caatttctta tgaagataat aaatctggat acctagctgg attaattgct ggaaaaatga
      541 ctgaaagcga taaggttggt tttataggag gaacaaagag ttctagtagg gataaatttg
      601 aatcaggttt tagagagggt gttaaatttt ctaatagtag tataagggat atttcagttg
      661 agtatgcaga tgtttttaaa gatagcaaat cagtagaatc tatagctaaa aaaatgatgg
      721 ataatggagt ggatattatt ttttctacta ctgaagatga ttcaaaagct gtaatagatg
      781 cagtaagggc aaaaaataaa aaagttatag caacgaataa agatcaacat gagttagctc
      841 cagaaaatgt tataagttct gttgtaaaag attttgagaa tcctacttat aatctgatac
      901 aaagctttgt aaaaggaaat tataaaggcg gaaaagttat agaaaacaca gttaagtctg
      961 gaagtactgg gcttgcccaa aatagttctc aaaatattcc tccagatgtg ttagaatatg
     1021 ttaataagaa caataaataa tctactataa tatccaagaa tacttatgac tttagttatg
     1081 aggtgaaatg gatgttataa tagacatata tagaaagaca tatgcagagg taggttagac
     1141 ctatctaaat cagggagtga attttgtgaa aataaaaagg atattgatgg cttgctttgt
     1201 agtagttttt acaattttta tattagctat atgtaatttt gattttggat ttgataaagt
     1261 aaataagaat aattctaata tagatagtgc taaatcgaaa cagaatgagt ctaaaagttt
     1321 gccaacttca gcagaaaagg ataaatcaaa tagcaaaagt aagaataaag aagatgacac
     1381 agaagatgat tcatatgtaa gaaaaactat tggagtattg ttagataccg aaaacttaaa
     1441 taacgaatca tataataaca atgtactatc agccttaaaa agggctgacg aagattttga
     1501 tgttaggata aaacttgaaa aaccaaagac ttctaaaagt tttaaagaga gtatagaaaa
     1561 actgcataag tatgaaccag atttgataat agcagttgga gccagatttg cagagccaat
     1621 taaagaagct gctactaaat atcccaaaca gcaatttgca atagttgact atacttataa
     1681 agttcagcca gctaatgtaa tttctgtatc atttcaagat aatgttactg gatatttagc
     1741 aggacttatt gcaggtaaaa tgactgatac tgggaaagtc ggttttatag gaggagttga
     1801 gggttctagt agagataagt atgaatctgg atttagaaag gggcttaaaa cttcaaataa
     1861 agatgctgag ttactagtta agtattcaaa tatatttgaa aaccctgaat cagctagaac
     1921 tattgcaaag gaaatgagag attctgatag agatgttgta ttttcagcta cagaggatga
     1981 tagcaaaaga gctattcagg tagcaagaga aaatggtggt aaagttatag ctattaataa
     2041 agaccaatac gatatggctc cagataatgt aataagttca attataaaag atgtagaaca
     2101 acctgtatat gaactgataa agaacgttgc aaaagatggt tttaagggaa gtaaagtaat
     2161 ggaatttaaa ataaaagatg gagaacttgg cttgacttca aagagcagta gaaatatacc
     2221 accagatgtt ttggaataca taaaaaaaga gtataacaaa gtgaaagata catattaatt
     2281 attgatgtag aagattataa aaatatactg cttaagttta gattggtgat aaaaggatat
     2341 atttattaaa tttagtctaa ctgtacaaaa tatattgatt gagtttaggt tagcaataaa
     2401 aagatatatt tattaaattt gtgctaactg tacggaatat actgcttaag tttagattag
     2461 tgataaaaag atatatttat taaatttgaa ccaactgtac aaagctatac taattaagct
     2521 tagattaact atataaaatt ataacaataa gtgtgtgggc agaaaaattc tgctacacac
     2581 tttatttata ttcacataaa aaatttatca aaaaaaagaa aaaggagtaa aaaatggaat
     2641 ttacaagaga accatttata ctagatgacg atttgtgtct agctttggtg gacaaaagaa
     2701 ttagttatct aatggaaaat gaattaaaat taagaaatat agagataata aaaacaatag
     2761 aatgtaaaga attatatgaa agcataaaat atcacccgga tatatgtatt tgtaatttag
     2821 gaaaaggaga tataattgtt gcgccgaatg tatataatca atacaaagag ttattaagta
     2881 gatataaatt taatataatt aagggagaag ctaatttatt aaatagatac ccttatgata
     2941 taccttacaa tattgcgata gttggtgact atgctataca caattttaag tatactgaca
     3001 aagcaatatt agattatatt gaaaataata aacttaaaaa gataaatata gagcagggct
     3061 attctaaatg ctcaatctgt atagtagata gaacgtctat aataacatca gataaaggta
     3121 tttggaaaag tatgaagaat acagaaatag agtgtttatt gatagaaaaa ggtcacataa
     3181 atttatttga gatgaattat ggatttatag gtggatgtac agggttgatt tctaaggata
     3241 aactagcttt ttgtggagat gttaaaaaac atcctgacta tgaacgtata aaaagttttg
     3301 tagaaagtaa aaacaaggag atagttacac ttagctgtga aaatttacta gatataggtt
     3361 ctatagttcc tctcatgaca agaaaggagc gataactttg gttgagaagg ataatatttc
     3421 ttccatgaaa tttttgagga ataataatgt aaacgataga ttaatatata cgaatgacat
     3481 tgtagaagta gtgatagatt ctgcaaagca agaagtgtta aataaaaaaa tacttaatgg
     3541 tactaaaact gaacaaagag ctttaaatat tgatgagata tcaacggaaa taactagtat
     3601 gataataagt ataatgaaag agaaactttt gaaagaatac attctaaaat gctatgggcg
     3661 tacatatcct gaagataaaa ataatatata catatattca ctgaacatat ttgctaaaaa
     3721 agagattttt atgagagaaa cagtatttac tagggtgaga aattatatag aagaaaatga
     3781 ttttataaat gtagaaggtt ttattagatt tagaatgaga gaatttatga aatatatttc
     3841 tgctataggt gatattgcag ttgaggagta tttaataaag aaagaccaag atgaatttat
     3901 aagagtttta aaatacttta tagatactca agaagaaaag attgatttgc ttaaagttca
     3961 tataatggaa gataatacat ttgttttata cgataaaaat ggaaataaga tagacagtat
     4021 agatgatgaa gaaattataa atatggtaat aagagaaaat ttaaattatg aggacttttt
     4081 aataagtacc ttattaactc tatgtcccaa aaaaatagaa atattggatt tgttaaataa
     4141 taattgctca aaggaaatta tagatactgt agaagcaatt tttgaaaata aagttagtat
     4201 aattatggaa aactagaatc taagcatata aaagctatcc atataaaatt aaaaaaattc
     4261 tttttaaatc gacacaaaag act
//
LOCUS       DAELLV010000269         4270 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.269, whole genome shotgun sequence.
ACCESSION   DAELLV010000269 DAELLV010000000
VERSION     DAELLV010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4270
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.269"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..384
                     /locus_tag="KPD58_003310"
     CDS             <1..384
                     /locus_tag="KPD58_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0267039.1"
                     /translation="NIIDIKRTGESKKIHINLEEWLHEVYNNLYGNSSTTKKFGEIIY
                     LNVLLTKEGVSKIKDSSWNLDMELNEDGSGIISTFIKVEDIDCYASILFFIGNNAKVI
                     EPKFLNKLLYDKANELRYFYEENMV"
     gene            434..1156
                     /locus_tag="KPD58_003311"
     CDS             434..1156
                     /locus_tag="KPD58_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HBG0267040.1"
                     /translation="MENFNKNAKIIGIGAEGINIINEVEEKIKANMDIEKININQEIE
                     KEYVRSLLDGVDILFLIYSSEDKHIRDIIKAVSYMSNERRVLSIGMDCSEKENKEDLE
                     LGREFKINNDSIFKFVDLMNIMVESISDSCMINIDITDLKEAIVGDKGIKYSFEEFED
                     TKSYSEIADILFDRMEYIGEEFISKKGIVFVEGSPEFSIMELNDLISNIQSKVEESYE
                     VIFSLYIKENLNGNIRVGLLYN"
     gene            1185..3119
                     /locus_tag="KPD58_003312"
     CDS             1185..3119
                     /locus_tag="KPD58_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0267041.1"
                     /translation="MSENIRGTEYWLMEEFFYQETDTPQIFREESIIALGFDIKINLK
                     KVFSMGKKSEKYINENCNDKDFIKKFMSIKKGDYFLYKAYQNIEEKVKCNVSIGIVEE
                     DFENGYELSHILGHTLPVKWMHNFDTVSNCKVYTSEFYKLNNNDISSYLEIIKSQDKK
                     LLRKRIEESENNKWYLKNYYYKNVDKCVDDEELFIQPINLEQLESLKNIKTDDYILLY
                     NSEKVICENDHTMKSLFGVCQVMEGFDKEKVSALEGGYFFPVRWIEYEKENIDGNAWV
                     IFENNSIVEKYVKRFTNKMPLNTILYGPPGTGKTYNTKQMVYNLVSETSLRNRYYTGY
                     LDYKNNVEFCTFHQSYGYEEFIEGLKSDGEGNFKPEDGILKDISIEACYNALKFEKKM
                     SFELEKDSLTLETIKDRKKKLVLENIEYSESFNFEESENYVLIIDEINRGNVSKIFGE
                     LITLLEDDKRLTKSNQTIVKLPYSKEKFSLPPNLYIVGTMNTSDKSIALLDIALRRRF
                     NFEEIMPDYDLLSIVDGIDIRKMLYTINKRIEFLYDRDHVIGQAYLLAVENINDIVVV
                     FRSKIIPLLQEYFYYDSEKVGLILGGIGRNEKDKYIVYKEKLIASELFKNNNASIMES
                     KVNYCIKQNISSEELKNIYE"
     gene            3112..>4270
                     /locus_tag="KPD58_003313"
     CDS             3112..>4270
                     /locus_tag="KPD58_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HBG0267042.1"
                     /translation="MSKNIYVKETYEWIRIGNGENELTEIEYEKLLKYLENNNDVLKS
                     NIIDIKYKKLRFINYVGIICFENVILEILPKLSLSDNLVKDREILLQMLSICNKIPIT
                     MNEKIRLSLKNYNLLNFFVMYFIESMQTQMKKGIYFEYINKIENLNVMRGKILLSTYA
                     KEKGISPMKIRCEYDEYSENNFLNQVLKKACISILCRINDNSIQGKIKKILSYFQNVD
                     LIYIDRKKLLDYKFYKNNDRFKDCYLLARLLLLNLSMDNSQNNQEAFSILFEINTLYE
                     EYIGILIKSIWDNSFRETYIQDKSKFLLKNEQTGKKNFNLRPDIVLYDLKNEYEIIID
                     TKWKAIEVDSNVFYRSSDIYQMYAYITAYENAKRCILLYPCIQKDKNYSSWK"
BASE COUNT         1832 a          312 c          731 g         1395 t
ORIGIN      
        1 aatataattg atataaaaag aacaggagaa agtaaaaaaa tacatataaa cttggaagaa
       61 tggttacatg aagtatataa caatttatat ggaaatagtt caactactaa aaaatttggt
      121 gaaattattt atctaaatgt attattaact aaagaaggtg tttctaaaat aaaggacagt
      181 tcttggaatt tagatatgga gctaaatgaa gatggtagtg gaataattag tacattcata
      241 aaggttgaag atatagattg ttatgcttcg attttatttt ttattggaaa taatgctaag
      301 gttatagagc caaaattttt gaataagcta ctttatgata aggccaatga gttaagatat
      361 ttttatgaag aaaatatggt ataatattta gaaatgttga aattaaataa ttattaagga
      421 gatatacgtt ataatggaaa attttaataa gaatgctaaa ataattggta ttggagctga
      481 aggtattaat ataataaatg aagtagaaga aaaaataaaa gcaaatatgg atatagaaaa
      541 aataaacata aatcaagaga ttgaaaaaga atatgttaga agtcttttag atggtgtaga
      601 tatattattt ttgatatatt catctgaaga taaacatatt agagatatta taaaagctgt
      661 ttcatatatg tcaaacgaaa gaagagttct atctatagga atggattgtt cagagaaaga
      721 aaataaagaa gatttagagc ttgggagaga gtttaaaatc aataatgata gtatatttaa
      781 atttgttgat ttaatgaata tcatggtaga gtctatttct gattcttgca tgataaatat
      841 agatattact gatttaaaag aagctattgt aggagataaa ggaattaagt attcttttga
      901 agagtttgaa gatacaaagt catatagcga gatagctgac attttatttg acaggatgga
      961 atatattggt gaggaattta taagtaagaa aggaattgta tttgttgaag gaagtccaga
     1021 attttctata atggaattga atgatttaat aagtaatatt cagagcaagg tagaagaaag
     1081 ctatgaagta atattttctt tatatataaa ggaaaattta aatggaaata ttagagtagg
     1141 cttattgtat aattagtata ttttagatat taaggagagt gtatatgtct gaaaatataa
     1201 gaggtacaga gtattggtta atggaagaat ttttttatca ggaaacagac acaccacaga
     1261 tttttagaga agaaagcata attgcacttg gatttgatat aaaaataaat ttaaaaaaag
     1321 tattcagtat ggggaaaaaa tcagaaaagt atattaatga gaattgtaat gataaagatt
     1381 ttataaaaaa gtttatgtcg attaaaaaag gagattattt tttatataaa gcatatcaaa
     1441 atatagaaga aaaagttaaa tgtaatgtgt ctattggcat agttgaagaa gattttgaaa
     1501 atggatatga attaagtcat attttagggc atacactacc agtaaaatgg atgcataatt
     1561 ttgatacagt ttcaaattgt aaagtatata caagtgaatt ttataaatta aataataatg
     1621 atattagctc atatttagaa attattaagt cacaagataa gaaattattg agaaaaagaa
     1681 tagaagaaag tgaaaataat aaatggtatt taaaaaacta ttattataaa aatgtagata
     1741 agtgtgtaga tgatgaagaa ttatttattc agcccattaa tctagaacaa ttagaaagtc
     1801 taaaaaatat aaagacagat gattatattt tattgtataa tagcgagaaa gtaatttgtg
     1861 aaaatgacca tacaatgaag agtctatttg gcgtatgtca agttatggaa gggttcgata
     1921 aagagaaagt aagtgctttg gagggtggat atttttttcc tgttagatgg atagagtatg
     1981 aaaaggagaa tatagacgga aatgcatggg ttatatttga aaataatagt atagttgaaa
     2041 agtatgtaaa aagatttact aataaaatgc cattaaatac tatactttat ggaccaccag
     2101 gaactggtaa aacatacaat acaaaacaga tggtttacaa tctagtaagt gaaaccagtc
     2161 taagaaacag atactataca ggttacttag attataaaaa caatgtagaa ttttgtacat
     2221 ttcatcaatc atatggatat gaggaattta tagagggtct taagtctgat ggtgaaggca
     2281 attttaagcc agaagatggg atattaaagg atatatctat agaagcatgt tataatgcat
     2341 tgaaatttga aaaaaagatg agctttgagt tggaaaaaga tagcttgact ttagaaacta
     2401 taaaagacag gaaaaagaaa ttagtattag aaaatataga atatagtgag agttttaatt
     2461 ttgaagaatc agaaaactat gttttaataa tagatgaaat taatagaggg aatgtatcta
     2521 aaatatttgg tgagctaata acattattgg aagatgataa aagacttact aaatcaaatc
     2581 aaactattgt caaactacca tattcaaagg aaaaattttc tctacctcca aatttgtata
     2641 tagttggaac tatgaacaca tcagataaat caatagcact tttagatata gctttaagaa
     2701 gaaggtttaa ttttgaagaa attatgccag attatgattt attgtctatt gtagatggta
     2761 tagatattag aaaaatgcta tatacaatca ataaaaggat agagttttta tatgatagag
     2821 accatgttat tggacaagct tatctacttg ctgtagaaaa tattaatgat atagtagtag
     2881 tatttagaag taaaataata ccacttcttc aagagtattt ttattatgat tcagaaaaag
     2941 taggtttgat tttaggagga attggaagaa atgaaaaaga taaatacatt gtttataaag
     3001 aaaaacttat agcttctgag ttatttaaaa ataataatgc ctcaataatg gagagtaaag
     3061 ttaactattg tataaaacaa aacatatcta gtgaggagct aaaaaatata tatgagtaaa
     3121 aatatatatg ttaaggaaac ctatgaatgg ataaggatag gtaatggtga aaatgagctt
     3181 acagaaattg aatatgaaaa attgcttaag tatctagaga acaataatga tgtattaaaa
     3241 agtaatataa tagatattaa atacaaaaaa cttagattta taaactatgt aggcatcata
     3301 tgttttgaaa atgtaatact tgaaatactt cctaaattgt cattaagtga taatcttgta
     3361 aaagatagag agatactatt acagatgctt tctatatgta ataaaattcc aattacaatg
     3421 aatgaaaaaa taagattgag tttgaaaaat tataatctct taaatttttt tgttatgtat
     3481 ttcattgaga gtatgcaaac acagatgaaa aaaggtattt attttgaata tataaataaa
     3541 atagagaact taaatgttat gaggggtaaa atattattaa gtacatatgc aaaagaaaaa
     3601 ggcatttctc caatgaaaat aagatgtgaa tatgatgagt atagtgaaaa taatttcttg
     3661 aatcaagttc ttaaaaaagc atgtatatct attttatgta gaataaatga caattcaatt
     3721 caaggcaaga ttaaaaaaat tttaagctat tttcaaaatg tagatttaat ttatatagat
     3781 agaaaaaaac tattggatta taaattttat aaaaacaatg atagatttaa agattgttat
     3841 ttactagcta gacttctact tttaaatttg tctatggata atagtcaaaa taatcaagag
     3901 gcattttcta tattatttga aataaatact ttgtatgaag aatatatagg aattttaatc
     3961 aaaagtatat gggacaattc atttagagaa acatatatac aagataagag taaatttctt
     4021 ttgaaaaatg aacaaacagg taagaaaaac tttaatttaa gaccggatat agttttatat
     4081 gacttaaaaa atgaatatga aattataatt gacactaaat ggaaagctat tgaagtagat
     4141 tcaaatgttt tttataggtc aagtgatata taccagatgt atgcatatat aacagcatat
     4201 gaaaatgcaa aaagatgtat attattatat ccttgtattc aaaaagataa gaattatagt
     4261 tcctggaaat
//
LOCUS       DAELLV010000270         4254 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.270, whole genome shotgun sequence.
ACCESSION   DAELLV010000270 DAELLV010000000
VERSION     DAELLV010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4254
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.270"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..1400)
                     /locus_tag="KPD58_003314"
     CDS             complement(<1..1400)
                     /locus_tag="KPD58_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0267043.1"
                     /translation="MTQKKLSRKEKVLICLKRLTEEHLNFNNGIRFGFDAEYIATEVG
                     INRSNASKELNQLIKESIIIKIKGKPVQYLYKESIESILGKKLNKGIFDNINSINEYS
                     ENDSIKDEYILESIKKENNIFNLVGCDGSLRSQIEQAKAAVIYPPKGLSTLIIGPTGV
                     GKSIFAEYMYKYSLTIKEDSDNAPFIIFNCADYSDNQQLLLAQLFGYVKGAFTGADKD
                     KYGIVHEANNGVLFLDEVHRLSAEGQEMLFLLMDKGIYRRLGEANKVHECRVMIIAAT
                     TEDPETAMLETFLRRIPVTIKIPSLEERGFQERMKLICYFFKEESVRIGVKLKVSKEV
                     IKAFILYKCKGNIGQLKSDIQLICARAFLDYMTYKRECVYVRLSLLPNNIRESLYGNN
                     RKEEFVQNFSFIGKDDIIFLPEEKDFESENLLIENKKYDLDFYGMIKETWNKLQQEGI
                     KESQIRSVIDGDIQKYS"
     gene            1566..2309
                     /locus_tag="KPD58_003315"
     CDS             1566..2309
                     /locus_tag="KPD58_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HBG0267044.1"
                     /translation="MNFLSDLHTHSIVSGHGYSTLLENINYCKENGIKILGTSEHGPK
                     MPGSPHKWYFHNIQNIPRIINNIIILRGCEANILDIKGNIDLEPFVIPRLDYLILSFH
                     EAVFSPNTLENNTKALINAINKHDNIEILGHLGNSNYPIDYELIIKLAIEKNILIEIN
                     NCSIKGVSRNGSSDNCKYIATLCKKYGAKIILTSDAHICFDIGNYEYSENILKEINFP
                     DELIMNYPKKLINHFHQKGKLLDVDYTNI"
     gene            3147..3878
                     /locus_tag="KPD58_003316"
     CDS             3147..3878
                     /locus_tag="KPD58_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0267045.1"
                     /translation="MLSKKNVSLIILVIILSLLTAIFIFNNKNTNNKLKEVTKNNEEI
                     VPKNLEGLSIEQLIEIRDKAIDKNLEGLREFGEDSVKKRKDSILNFEFGLVAFVNKNY
                     SNLTEKEKLIRQINIVNYENSINDIMDPSGSFFELANQNYQVNEFLLKNGKDYKEWSY
                     EGLDFLIDHLEELCKFVDFDVKDVIDIIDPTIKTDLSEEQKKSLNDKLKKISSSDTLS
                     EEVKKEIKNWENNLNNLNMNKNNSN"
     gene            complement(4035..4241)
                     /locus_tag="KPD58_003317"
     CDS             complement(4035..4241)
                     /locus_tag="KPD58_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0267046.1"
                     /translation="MSKYSSLWEYVKKNNSQSFKLTFEEIKDIAGIEIDHSFLKYKKE
                     LNEYGYQVGKISLKEKTVIFDKIG"
BASE COUNT         1591 a          613 c          450 g         1600 t
ORIGIN      
        1 gaatacttct gtatgtctcc atctataaca cttctaattt gtgactcttt aataccttct
       61 tgttgaagtt tattccaagt ttcttttatc attccataaa aatctaaatc atatttttta
      121 ttttctatta ataaattttc actttcaaaa tccttttctt ctggtaaaaa aataatatca
      181 tcttttccaa taaaactgaa attttgtaca aactcctctt ttctattatt tccatataat
      241 gattctctaa tattatttgg tagaagagag agccttacat atacacattc ccttttatag
      301 gtcatataat ctaaaaaagc tcttgcacaa attaattgta tatcgctttt taattggcct
      361 atgtttccct tacacttata taaaataaat gccttaataa cttcttttga aacctttaac
      421 ttaacaccaa tcctaacact ctcttcttta aaaaaataac atataagctt cattctctct
      481 tgaaagcctc tttcctctaa agacggaatc tttatagtta caggtattct tcttaaaaac
      541 gtttccaaca ttgctgtttc tgggtcttct gtagtggctg caattatcat aactctacac
      601 tcgtgtacct tattagcctc tcctaacctt ctatatatcc ctttatccat aagcagaaat
      661 agcatttctt gtccctctgc agataatcta tgaacttcat ctaaaaacaa tactccatta
      721 ttagcttcat gtacaatacc atacttatct ttatcagctc cagtaaatgc tccttttaca
      781 tatccaaaaa gctgcgctaa taataattgc tgattatcag aataatcagc acagttaaat
      841 atgataaatg gtgcattatc actatcttct tttatagtta aactatactt atacatatat
      901 tctgcaaata tagatttacc aactcctgta ggtcctatta ttaatgtact caacccctta
      961 ggtggatata ttactgctgc tttagcctgt tctatttggg accttaaact tccatcacat
     1021 cctactaaat taaaaatatt gttctctttt tttatactct ctaaaatata ctcatctttg
     1081 atagaatcat tttcactata ttcgtttata gagttaatat tatcaaatat ccctttattt
     1141 agtttctttc ctaaaatact ctcaatactt tctttatata agtactgaac aggcttaccc
     1201 tttatcttta taataatact ttcttttatt aattgattta attccttact agcattactt
     1261 ctatttatac ctacttcagt agctatatat tctgcatcaa atccaaatct aatgccatta
     1321 ttaaagttca agtgttcttc tgttaacctt ttcaagcaaa ttaaaacttt ttctttcctt
     1381 gatagcttct tttgtgtcat agttaacctc ccctataagc agtaatactg ttaaacattt
     1441 attattaagc ttaattctac aatattttaa tatttttgtc taattatgta actattgatt
     1501 ttctttccta ttcgttttat aatataaatt acgttaccct catcttttat agaggaggaa
     1561 tttatatgaa ttttttatca gatttacaca cccactctat agtcagtggt catggctata
     1621 gcactttact agaaaatatt aattattgta aagaaaatgg aattaagata ctaggtacat
     1681 ctgaacatgg tcctaagatg cctggttctc cccacaaatg gtactttcac aacattcaaa
     1741 atatcccaag aatcataaat aacattataa ttttaagagg ctgtgaagca aatatactag
     1801 atattaaagg aaacatagac ctagagcctt ttgtaattcc tcggttagat tatcttatct
     1861 tgtcatttca cgaagctgtg ttttctccaa atacattaga gaataatact aaagctttaa
     1921 taaatgcaat aaataagcat gataatattg aaatattagg acaccttgga aactcaaact
     1981 acccaatcga ctatgaatta atcattaaat tagctataga aaagaatatc ttaatagaaa
     2041 taaataattg ttctattaaa ggcgtgtcaa gaaatggtag ctcggataac tgtaagtata
     2101 ttgctacact ttgcaaaaaa tatggtgcta aaatcatact gacttcagat gctcacattt
     2161 gttttgacat aggtaactat gagtattctg aaaatatctt aaaggaaatt aattttccag
     2221 atgaattaat aatgaattat cctaaaaaac ttattaatca ttttcaccaa aaaggtaaat
     2281 tactagatgt tgattataca aatatctaag taataataca tgcttacttt aactcccata
     2341 aaaattttaa tactatttat attatatgaa ataaagaaat tataataaag aagctattta
     2401 ataaagctta attactttta tgaacttgat ggattgatat ggtctatgat agcaatagaa
     2461 attataacat ttttaataaa cttagtttta tataccttat agtataaatc taaaaaagca
     2521 tcatctaaaa aggagctgtc acatgagcaa attaaatttg ttctatgata gctctttttt
     2581 tacttatttt aattaggtat atttttaatt attttatata aagcttatgt tctaagtaaa
     2641 attatgcata acaaacaaag gtataaaaat accatttttt cagataatat ttaattataa
     2701 ataataaatt tagaatactt cttttaaatg atgagagtct agaaattttt atgtgtattc
     2761 tttaaataaa aatttccaga ctctagcccc tttttgaata ttttaaaatt taacttaaac
     2821 taagcaaatt ctataatagc ttatctctaa atatttaaat ttaatttatc atacaatatt
     2881 acttattata aaacttaaaa catataaaaa tatattttaa agcaataatt ttattttact
     2941 tattaaatat ttggagttat taaaatcttt tctatctaaa aaatattgat attaaatatc
     3001 taataagaaa atttaataag aaaaatatag attatatcaa aaaaatattc tattataatc
     3061 tttagaacaa acagtagtaa atgttatctt cataaacaaa tttatttttc acaagtacaa
     3121 tacttattta aaaaaggagg tttaatatgt taagtaaaaa aaatgtaagt ttaattattt
     3181 tagtaataat tttaagttta ttaactgcta tatttatttt caataataaa aatactaaca
     3241 ataaattgaa agaagttact aagaataatg aagaaatagt acctaaaaac ttagaaggtt
     3301 taagtataga acaattaata gagattagag ataaagccat tgataaaaat ttagaaggtc
     3361 ttagggaatt tggagaagat tcagtaaaaa aacgtaaaga ttcaatttta aactttgaat
     3421 ttggattagt agcttttgtt aacaagaatt atagcaactt aacagaaaaa gagaaattaa
     3481 taagacaaat taatattgtt aactatgaaa actctataaa tgatataatg gatccttcag
     3541 gttcattctt tgaattagca aatcagaatt accaagtgaa tgagtttcta ttaaaaaacg
     3601 gaaaagatta taaagaatgg tcatatgaag gtctagattt tctaattgat catctagaag
     3661 aactgtgcaa atttgttgat tttgatgtaa aagatgtaat agatattatt gatcctacaa
     3721 taaaaacaga cctttcagag gaacaaaaga aatctttgaa tgataaatta aagaaaatat
     3781 cttcaagtga tactttaagt gaagaagtta aaaaggagat aaaaaactgg gaaaacaatt
     3841 taaataattt aaatatgaat aaaaataatt ctaattaatt ttctcataag aaaaatgctt
     3901 atatcgtgat aatgtgaaat gtacataatt ataaacaaaa aaggggctgt gacactaaaa
     3961 aatagtgcta cgcccttttt tatttgtaat aatcatcaat acaaattatt ttattatatg
     4021 taattattta tgtcttaacc aattttatcg aaaataacag tcttctcttt taaagagatt
     4081 tttcctactt gatatccata ttcatttaat tcctttttat atttcaaaaa cgaatggtct
     4141 atctctatcc cagcaatatc ttttatttct tcaaaagtta atttaaacga ttgactattg
     4201 tttttcttaa catattccca taaagaacta tatttactca tcttatatca ctcc
//
LOCUS       DAELLV010000271         4177 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.271, whole genome shotgun sequence.
ACCESSION   DAELLV010000271 DAELLV010000000
VERSION     DAELLV010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.271"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            91..771
                     /locus_tag="KPD58_003318"
     CDS             91..771
                     /locus_tag="KPD58_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBG0267047.1"
                     /translation="MKKQYVFIILTSTILGILVGTIFRDYSNEETVYTSKDKSIRKEI
                     KMTRKSIKELTKEKDKLDKEINSMKQNQKNSEEISEIETLRDSLSYSDVKGSGIILSI
                     DAIDEKTGNIANSIDYNKILVKVINELKVNGGKYIEINGQRINQYSEVVLAGSHININ
                     GVPVAQPYEIKCIGDKEKLSNYVDKGSKYLDSIQTNYPMKISIKTEENISMKKMNIPN
                     KLKYIKGD"
     gene            764..2143
                     /locus_tag="KPD58_003319"
     CDS             764..2143
                     /locus_tag="KPD58_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HBG0267048.1"
                     /translation="MIKIILEESSLPMLIPEKLAKKYNVFPVEFQDDNLVVEIEDEDI
                     YALQDLRLATGKEIILKKEKKELISEKIEKYYNSFELDEDYAKKLFENLLEKAIKENA
                     SDIHIEPFKEHLIVRMRVDGELKEVSKFLVDVYPSLSTVIKLKASMDITEKRLPQDGR
                     VDIKLGGNLIDIRVSSIPTVYGEKIVLRILNRDTFLKDKLELGFSEEAIKSINRIINK
                     RAGILLVTGPTGSGKTTTVYSLLNDLKGINKNIMTIENPVEYKMDGISQIQVNSKVGL
                     SFDVGLRAILRQDPDIIMVGEIRDAETAKIAVRAAITGHLVISTLHTNDAVSSIARLL
                     EMQIPPYLLNASLIGVISQKLVRKVCNHCSHEIMIKDNFSGDVNTKVAVGCEKCNDKG
                     YFGRTAIYEILEINDDIKTCIRNMEDSSTIKDVAQNNGMITFEDSCKRLINEKITTLE
                     ECLVVNEMI"
     gene            2314..3399
                     /locus_tag="KPD58_003320"
     CDS             2314..3399
                     /locus_tag="KPD58_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBG0267049.1"
                     /translation="MRLSKNIFYNLEKFYLVLNRISDKEIRVICKEIGILLESGCEIT
                     KIFEIIESQSSKKAKNLLSIVSNHIQKGNSIAESFQITGIFSKFFISMIKAGETSGNL
                     DIIMSDLSNYYDKEYKLKMKIITISIYPIILIILSILSMLFIFVFVIPNFQVVFTNNG
                     IEPPLITRVLMGISTVVTNNLAYIIFSFILFSIGSIYFFITNDNIKKLINSLKFKIPF
                     IKKINQLVATTRFSRALSVLISSGIQIVEAIDMSASVIDNEFIYERLKISNNYIKKGN
                     SIGKSLNLANVFPKLFISMANIGEESGKLDKSLNVINKFYENELNNKIEQIMKFIEPM
                     LIVFMGLIIGTFILAMVVPMFDAITNF"
     gene            3453..3812
                     /locus_tag="KPD58_003321"
     CDS             3453..3812
                     /locus_tag="KPD58_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBG0267050.1"
                     /translation="MINLINKKRKGFTLVEMIVVVTILGVISSIALVKYSKVQESAKL
                     NADYTNAANIVTAASMAINDDEKTIDSLSVETLKEKGYLNTVPVPQSTSGKFELVIND
                     SGTDISVNINSKQFYPK"
     gene            3865..>4177
                     /locus_tag="KPD58_003322"
     CDS             3865..>4177
                     /locus_tag="KPD58_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HBG0267051.1"
                     /translation="MAIIKKILNLEINDDYIRLVFLRKGHKKVFIDKSIIKEINFDIK
                     NDSNSIEKIKYIEDVVRFVKEEIGKSKILYKNLYFNIQIQDIVIRNVEVLNTKKKRDI
                     LS"
BASE COUNT         1795 a          373 c          695 g         1314 t
ORIGIN      
        1 taactttgat ataataagca tatgtaaatg tttaatattt aattaaagaa aattatcata
       61 taaaaataaa tttgtaagga gcaatacagt atgaaaaaac aatatgtgtt tataatacta
      121 acaagtacta ttttaggtat acttgttgga accatattta gagattatag caatgaagaa
      181 acagtttata caagcaaaga caagtcaata agaaaagaaa taaaaatgac aagaaaatct
      241 ataaaagaat taaccaaaga gaaagacaag ttagataaag aaattaattc aatgaaacaa
      301 aatcaaaaaa attctgaaga aatatcagaa atagaaacac ttagagatag tctatcatat
      361 tcagatgtaa agggtagtgg gataatatta agtatagatg ctatagatga aaaaacaggg
      421 aatatagcta atagtataga ctataataag attcttgtaa aagttataaa tgaattaaaa
      481 gtaaacggag gaaaatacat agaaataaat ggtcaaagaa ttaatcaata ttcagaagtt
      541 gtacttgcag gaagccatat aaatataaat ggagttcctg tagcacaacc atatgaaata
      601 aagtgcattg gagataagga gaaactatct aattatgtag ataaaggtag taaatattta
      661 gatagcatac aaaccaatta tccaatgaaa ataagcataa aaactgaaga aaatatctct
      721 atgaaaaaaa tgaatatacc taataaatta aagtatataa agggtgatta agattatatt
      781 agaggaaagc agtttgccaa tgttaatacc agaaaaactt gcaaaaaaat ataatgtatt
      841 tccagttgag tttcaagatg ataatttagt tgtagaaata gaagatgagg atatttatgc
      901 tctacaggac ttgagattag ctacaggtaa agaaattata ttgaagaagg agaaaaaaga
      961 acttataagt gaaaagattg aaaaatatta taatagcttt gaacttgatg aggattatgc
     1021 aaaaaaatta tttgaaaatc ttttagaaaa agctataaaa gaaaacgcaa gtgatataca
     1081 tatagaacca tttaaagaac atttaatagt gagaatgagg gtagatggag aactaaagga
     1141 agtatcaaag tttttagttg atgtctatcc atcattgtct acggttataa aattaaaggc
     1201 atcaatggat ataacagaaa aaaggcttcc tcaggatggt agggtggata taaagttagg
     1261 tggaaattta attgacatta gagtttcatc aataccaact gtctatggtg aaaaaatagt
     1321 tttgaggata cttaatagag atactttttt aaaagacaaa ttagaacttg gtttttcaga
     1381 ggaagctata aaatctataa atcgtataat aaataaaaga gcaggaatat tattagtgac
     1441 aggtcctaca ggtagtggaa agactacaac agtatattcc ttattaaatg atttaaaagg
     1501 aataaataaa aatattatga ctatagaaaa tcctgtagag tataagatgg atggcataag
     1561 tcagattcaa gtaaattcaa aagtagggtt gagctttgat gtaggtctta gagcgatatt
     1621 aagacaagac ccagatataa taatggtcgg agaaataaga gatgcagaaa cagctaagat
     1681 agcagtaaga gcagctataa caggtcattt agttataagt actttacata ctaatgatgc
     1741 agtatcttcc atagctagat tactagaaat gcaaatacca ccatatcttt taaatgcctc
     1801 tcttataggt gttatatctc aaaagttggt tagaaaagtc tgcaatcatt gtagccatga
     1861 gattatgata aaagataatt ttagtggaga tgtaaatact aaggtagctg tagggtgtga
     1921 aaaatgtaat gataaaggat actttggaag aacagcaatt tatgaaatct tagaaattaa
     1981 tgatgatata aagacatgta taagaaatat ggaggattct agtactatta aagatgtggc
     2041 acaaaataat ggaatgataa catttgaaga tagttgtaaa agactaataa atgaaaaaat
     2101 aacgacatta gaagagtgtt tagtagtaaa tgaaatgatt taactgaatt gtatagttaa
     2161 acttcgtttg gtcagttgtc tttatttaat ctattttaat taagatgttt gagtataaat
     2221 tatatttaag tgaaataaaa ttaaatgcat taaattagaa aatattaagt ttatttaatt
     2281 gtaattaata taatttggaa ggtattatag tacatgagat tatctaaaaa tatattttat
     2341 aatttagaaa agttttattt agtattaaat agaatcagtg ataaagaaat tagagtaatt
     2401 tgcaaagaga taggcatatt gctggaatct ggatgtgaaa ttacaaaaat atttgagatc
     2461 attgagagtc agtcaagtaa aaaagcaaag aatttgctta gtatagtatc aaatcatatt
     2521 caaaaaggta actcaatagc agaatcattt caaataacag gtatattttc taaattcttt
     2581 ataagtatga taaaagcagg agaaactagt ggaaacttag atataataat gagtgattta
     2641 tctaattatt atgataaaga atacaaatta aaaatgaaaa taataactat ttcaatatat
     2701 ccaataatac ttataatatt atctatctta tcaatgttgt ttatattcgt atttgtaata
     2761 cctaattttc aggtggtttt taccaacaat ggaattgaac cacctttaat aactagagtt
     2821 ttaatgggaa tatctacagt ggttacaaat aacttagctt atataatatt tagttttata
     2881 ttgttttcaa taggaagcat ttatttcttt ataacaaatg ataatataaa aaaattgatt
     2941 aattcgttaa aattcaaaat accttttata aaaaaaatta atcagttagt agctacaaca
     3001 agattttcaa gggcattgtc cgttttaatt agcagtggaa tacaaattgt ggaagctata
     3061 gacatgtcag ctagtgttat agacaacgaa tttatttatg aaaggttaaa gatttccaat
     3121 aattatataa aaaaaggaaa tagcataggt aaatctttga atttagcaaa tgtttttcca
     3181 aaattattta tttcaatggc aaatatagga gaagaaagtg ggaaactaga taaaagtcta
     3241 aatgtaataa ataaatttta tgaaaatgaa ttaaataaca aaatagagca aataatgaaa
     3301 tttatagaac ctatgctaat tgtatttatg ggattaatta ttggtacttt tattttagca
     3361 atggttgttc ctatgtttga tgcaattact aatttttaga gagaaaaata aattttatta
     3421 gatgaaactt ttgaaaagga gagaatattt aattgataaa tttgataaat aaaaaacgaa
     3481 aaggatttac acttgttgaa atgattgtag tagtaactat tttaggcgtt atatctagta
     3541 tagcattagt taagtatagt aaggttcaag aaagtgccaa attaaatgca gactatacga
     3601 atgctgctaa tatagtaacc gcagctagca tggcaattaa tgatgatgaa aagacaatag
     3661 actctctaag tgtagagaca ttgaaggaaa agggatacct aaatactgtt ccagttcctc
     3721 agagtacatc aggtaaattc gaacttgtca taaatgatag cggaacagat ataagcgtaa
     3781 atataaattc taagcaattt tatccaaaat aatattttat attgattgct gtaagtttta
     3841 aaattatttt tatttaagaa ggagatggct ataattaaaa aaattctaaa cttagaaata
     3901 aatgatgatt atataaggct tgtattttta agaaaaggtc ataaaaaagt ttttatagat
     3961 aaatcaatta taaaagagat taactttgat ataaaaaatg atagtaattc gattgagaag
     4021 attaagtata tagaagatgt agttagattt gtaaaagagg agattggtaa atcaaaaata
     4081 ttatataaaa atttatactt taacattcaa attcaggata tagtcattag aaatgttgaa
     4141 gttctaaata ctaagaaaaa aagagacata ttaagca
//
LOCUS       DAELLV010000272         4170 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.272, whole genome shotgun sequence.
ACCESSION   DAELLV010000272 DAELLV010000000
VERSION     DAELLV010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.272"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..429)
                     /locus_tag="KPD58_003323"
     CDS             complement(<1..429)
                     /locus_tag="KPD58_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HBG0267052.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEKNWADE
                     HRIGVAQGVDEEIIEGLRANFVGECTEVGMYLAMSRQADREGYPEVGEAYKRIALEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAK"
     gene            complement(547..2370)
                     /locus_tag="KPD58_003324"
     CDS             complement(547..2370)
                     /locus_tag="KPD58_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0267053.1"
                     /translation="MNLSTGNQKVSRLKKKEKPKDFKKTFKRIFMYFKKDKKITRFIF
                     LLLIIDSIISTVIPYTIGKVVDIIDIDTYSKSIFLKGIIILLSFYIIEFSVKLSKGIC
                     TARLSQSIVKEMRSNLFLKFKNLPITFFDKSSTGDIMSRVTNDVDNISTGISNSLVQL
                     ITGILNILMTFIMMFVLSPILTFCSVILIPIVMFVSNFIAKRTRVFFKQQQVELGKLN
                     AHIEEMISNINVVKSFNYERKSVNDFKEVNDRLLNISLKSQIYTSLLMPIMNVISNIG
                     FATICIIGGILASKDIITIGVIASFLSYVRQFTRPLNELANLFNTLLSAIAGCERVFE
                     IIDEQEEQEVHNMFKSEINDNKDGNLNSHFVKGEIELKDVSFSYDSKKKILDNINLKI
                     KSGTSNAIIGETGSGKTTIINLITNFYHIDEGSIFLDGKDIYSINRNYLRTCFGVVPQ
                     EGYIFNDTVMENIRYGNLEATDEEVIYASKIANAHKFIVKMREGYSTVLSDRGEDLSE
                     GQKQLISIARAVLKNAPILILDEATSNIDIATEAKIQEAINNLTYGKTSIIIAHRLST
                     ISNCDNIIVLENGRIVESGNHKELIKLKGHYYRNILLTQGK"
     gene            complement(2383..4158)
                     /locus_tag="KPD58_003325"
     CDS             complement(2383..4158)
                     /locus_tag="KPD58_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0267054.1"
                     /translation="MRYFKKYIKKYIVLFLTAVFFLTLEAFCDLAQPTIMAKIIDIGV
                     SNKNLDYILSTGAIMIGITFLGAIFAIIRSVISARVSQNFSYDLRQDVFEKINTYSLK
                     QIDKFGRASLITRITNDINQMQQFVNGMMRVFIKSPIMCIGSLIIAIKLNLKMSIIIL
                     IAVLIIFIIILLNMKIGYRLFKNVQESTDKVNSKLRQFLSGIKVVKLFCRYDYENQSF
                     EELNDELFKNSRKATTMMSFFRPTITIIINVSIIIILLIGRHLIYSGEIKIGIIVAYV
                     NYMTRILTSLIMISHVFNVFVRAKASYERISEVLVDENEEVIEREESLDVGEKDLKFV
                     NCDIIFENVDFSYNKNTDEKILKNVSFKIEEGQNIGVIGQTGSGKTSLVNLIQKLYII
                     DKGDIKIGGRSIYSISEESLRARIGVVFQNSSVFGKSILENVVMGNRDICKEDVVKSC
                     EIANANQFITKLENTYDEVLFQRGKNLSGGQRQRITIARAIAKKPQILILDDCFSAMD
                     INTETIIRKNIKEYISNLREKESKKCTVITISQKISSIIDCDKILVLDEGEVVGFATH
                     EDLLVSSPIYKKIFALQNLVSEDVN"
BASE COUNT         1349 a          751 c          372 g         1698 t
ORIGIN      
        1 cttagctaat tttaattttc catcagttgc accatactca gcatcaactc taactcttaa
       61 gttttctttt gtatctgcaa ctacaacttc tccaagaagt tctgcaaatt tagcagcatg
      121 ttcagcttct tcaagagcta ttctcttata agcttctcct acttctggat aaccttctct
      181 atcagcttgt ctactcattg ctaaatacat tcctacttct gtacactcac caacaaagtt
      241 agctcttaat ccttcgatta tttcttcatc tacaccttga gctactccta ttctgtgctc
      301 atcagcccaa tttttttcgc ctttcatttc ttcaaattta tcagctccaa ctttacatac
      361 tggacattgt gctggtgcag catctccttc atgtatatat ccacatactg tacaaacaaa
      421 tttcttcata attactttcc tcctaaattt tatatatcaa attaaatttt ttatttatta
      481 agttagtcta tatatacccg caccatatac ctttaaacaa tttttttaaa agttttttct
      541 aaatacttat tttccctgtg tcaataatat gtttctataa taatgtccct ttaattttat
      601 caattcttta tgatttcctg attccacaat tctaccattt tctaaaacta ttatattatc
      661 acaatttgat atggtactta atctatgagc tataattata cttgtctttc cataagttaa
      721 attatttatt gcttcttgaa ttttagcttc tgttgctata tctatgttac ttgtagcctc
      781 atccaaaata agtattggag cattttttaa tactgctcta gcaatactta ttaattgttt
      841 ttgaccttca cttaaatctt cacccctatc cgataataca gtactatatc cttcacgcat
      901 tttaactata aatttatgtg catttgctat tttactagca tatataactt cttcatcagt
      961 agcctccaga tttccatacc ttatattttc cataactgta tcattgaata tataaccttc
     1021 ttgtggaact actccaaaac aagttctcaa atagtttctg ttaatagagt atatatcctt
     1081 accatctaaa aatatacttc cttcatctat atgataaaaa tttgtaatta gatttataat
     1141 tgttgttttt ccacttcccg tctctcctat gatggcattt gatgtgccag atttgatttt
     1201 taggtttata ttatctaaaa ttttcttttt tgaatcataa gaaaaactta catctttaag
     1261 ctcaatttct ccctttacaa aatgagagtt taaattacca tctttattat cgttaatctc
     1321 acttttaaac atgttatgca cttcttgctc ttcttgttca tctataattt caaaaactct
     1381 ttcacatcca gctatggctg aaagtaatgt attaaataga tttgctaatt catttaaagg
     1441 tcttgtaaat tgtcttacat aacttaaaaa actagctata acccctatgg ttataatatc
     1501 tttagatgca agaatgcctc ctataataca tatagttgca aatcctatat tactaatcac
     1561 attcataata ggcattaaaa gagaagtgta aatttgcgat ttaagggaaa tatttagaag
     1621 tctatcattg acttctttaa aatcatttac agattttctt tcataattaa aagattttac
     1681 tacatttatg tttgatatca tttcttcaat atgtgcattt agttttccca actcaacttg
     1741 ttgttgctta aaaaaaactc tagttctctt tgcaataaaa ttactcacaa acattactat
     1801 cggtattaat attacactgc aaaaggttaa aattgggctt aatacaaaca tcataataaa
     1861 agtcatcaat atgtttaaga ttcctgtaat taactgtact aatgaatttg atattcctgt
     1921 actgatatta tcaacatcat ttgtcactct actcataata tctccagtag aacttttatc
     1981 aaaaaaggtt attggtagat ttttaaattt taaaaataaa ttacttctca tttctttaac
     2041 tatactttga gataatctag ctgtacatat ccccttagac agtttaactg aaaactcaat
     2101 tatataaaat gaaagtaaaa taattattcc ttttaagaaa atggattttg aataagtatc
     2161 gatatctatt atgtcaacta cctttccaat agtatatggt attacagttg atataattga
     2221 atcaattatt aatagcaaaa aaataaatct agtaatcttc ttatcttttt taaagtacat
     2281 aaaaattctt ttaaacgttt ttttaaaatc tttaggcttt tcttttttct ttaatctact
     2341 gaccttctga tttccagtac ttaaattcaa tatacttcct ccttaattta catcttcact
     2401 tactaaattt tgcaacgcaa aaattttttt ataaattgga gaactaacca gtaaatcttc
     2461 atgtgtagca aatccaacca cctcaccctc atccaagact aaaatcttat cacagtcaat
     2521 tatagaacta atcttttgtg aaatagttat tacagtacat ttcttacttt ccttttctct
     2581 taaattactt atatactctt ttatattctt tcttataatt gtctctgtat taatatccat
     2641 tgcactaaaa caatcatcaa gaattaatat ttgtggtttt ttggctatag ctctagctat
     2701 agtaattctt tgtctctgtc ctcctgataa atttttaccc ctttggaata aaacttcatc
     2761 ataagtattt tcgagttttg ttataaattg atttgcattt gctatttcac atgattttac
     2821 aacatcttct ttgcatatat ctctatttcc cataactaca ttctccaata tactttttcc
     2881 aaacacacta ctattttgaa atactacacc tattcttgcc cttaaacttt cttcagaaat
     2941 actgtatata ctacgtcctc caatcttaat atcaccttta tcaattatat ataatttttg
     3001 aattaaattt actaaagaag tctttcctga accagtttga ccaatcacac caatattttg
     3061 accttcttct attttaaagg atacgttttt taatattttt tcatctgtat ttttgttata
     3121 cgaaaaatct acattttcaa aaattatatc acaatttaca aactttaaat ctttttctcc
     3181 aacatctaaa ctttcttctc tctcaattac ttcttcattc tcatcaacca aaacctcact
     3241 tattctctca taagatgctt ttgccctaac aaaaacatta aaaacatgtg aaatcattat
     3301 aagagatgtt aatattctag tcatatagtt tacataagca actataatcc caattttaat
     3361 ttctcctgaa taaattaaat gccttccaat tagtaatatt attattatgg aaacattaat
     3421 gattattgta attgtcggtc taaaaaatga catcatagtt gtagcttttc tactattttt
     3481 aaaaagttca tcatttaact cttcaaagct ttggttctca taatcgtatc tacaaaatag
     3541 tttaactact tttattccac ttaaaaactg tcttaatttt gaatttactt tatctgtact
     3601 ttcttgtaca tttttaaata atctataccc tatcttcata ttaagcaata ttataataaa
     3661 tataattaaa actgcaatta gaataattat tgacatcttt aaatttaatt ttatagctat
     3721 aattaaactt cctatacaca ttattggtga ctttataaaa actctcatca ttccattaac
     3781 gaattgttgc atttgattga tatcattagt aattcttgtt attaatgaag ctctaccaaa
     3841 tttatctatt tgttttaatg agtatgtatt tatcttttca aatacatcct gtcttaaatc
     3901 ataagaaaaa ttctgagaaa ctcttgccga gattacactt cttattattg caaatattgc
     3961 acctaaaaaa gttataccta tcattatagc tcctgttgat agtatgtagt ctaagttctt
     4021 atttgaaacc cctatatcta ttatttttgc cattatagtt ggttgagcta aatcacaaaa
     4081 agcttccaat gttaaaaaaa atactgctgt tagaaataat actatatatt tttttatgta
     4141 ttttttaaaa tacctcattc aatcatccct
//
LOCUS       DAELLV010000273         4161 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.273, whole genome shotgun sequence.
ACCESSION   DAELLV010000273 DAELLV010000000
VERSION     DAELLV010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.273"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            253..2352
                     /gene="nrdE"
                     /locus_tag="KPD58_003326"
     CDS             253..2352
                     /gene="nrdE"
                     /locus_tag="KPD58_003326"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBG0267055.1"
                     /translation="MNWIELNNQVIIKNEQGKYQLEKDKEALSSYIEEFIQPKLKKFN
                     NLEERLKYLIDEGYYSKEVINQYSIDVIKNIYDMIDKHKFEFQSYMSANKFYQNYALK
                     SNDGKEILETYNDKVLIVALTLGNGNKDLALNLADKIVKQEFQPATPTFLNAGRKRAG
                     EMVSCFLLSVEDSTEGISYAISSSNHLSKIGGGVALNLSKLRASGESIKDIEGAAGGV
                     VGVAKMLEQSFSYFNQMGARQGSGAVYLTVFHPDFELLMDTKKINADEKIRLATLSLG
                     AIIPDKFMELAEKNEVAYAFYPHTVYKKYGVSLDEIEMDEWYDKLVNDSDIRKKEINP
                     RQMLTKIAQMQQESGYPYVVYIDTANREHTLKDVGIIKMSNLCCEIFQYQTPSEIEGY
                     GGKNEWGQDISCNLGSLNIANVMDNKTIESTVETAIRALSFVADSTDIKPVPTVSNSN
                     SKSHSIGLGAMNLHGYLVRENILYTSDDAIDFSNVFFAMVRYYSIKASMKIAIERNQT
                     FEGFDKSEYVKGRNSKVLSKYYEQSYLPKSEKVRALFEGIYIPTKEDWTKLLDEVKEK
                     GIYNAYLMAVAPTQSISYVQNATSSIMPITEPVEVRTYGDSTTIYPMPFLTNDNMLYY
                     QSAYRMDMRKVIDLVATVQNHVDQGISTTLFVTDEKTTRDIARHYIYAYKKGLKSLYY
                     TRTKMTRDTHECLVCSV"
     gene            2365..3336
                     /gene="nrdF"
                     /locus_tag="KPD58_003327"
     CDS             2365..3336
                     /gene="nrdF"
                     /locus_tag="KPD58_003327"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBG0267056.1"
                     /translation="MTFNLHKIHNAVNWNREEDGFTQAFWEQNVKQFWLPEEISVSKD
                     IKVWNELSNKEKELYKKVLGGLTLLDTKQGNNGIPSMMSLTENLQRKAVLSFMGTMEE
                     IHAKSYSSIFMTLLSNLEIDELFEWIETEPTLQRKADLVLAQYENTTNQEGLYLSMVT
                     SVFLESFLFYSGFFYPLYLSGQGKMVASGEIISLILRDESLHGKYIGLLAQEIYDSFD
                     KMDKEMLEEKMYSILYSLMENEIEYTNVIYRESGLEKEVVNFLKYNANRALENLGFEK
                     LYTVDAINPIVLNGLSTETKTHDFFSTKGNGYQKGVYEELEDEDFII"
     gene            3412..4038
                     /locus_tag="KPD58_003328"
     CDS             3412..4038
                     /locus_tag="KPD58_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBG0267057.1"
                     /translation="MKYEWRKRDKELYLPKKNPVIVEIPEQKFIMLKGSGDPNSKEFT
                     EAIGVLYSLAYAIKTMPKKGIVPEGYFDYTVFPLEGIWDKGEKSKGLDVLIKEDLIYT
                     IMIRQPDFVTQDLLDKATDIVKKKKPHNLLEKVRFGSINEGLCVQKLHIGSYDNEPES
                     FDIMNEFCKENKLIRKSYTHREIYISDARKVSPESLKTVLRFEVEREV"
BASE COUNT         1670 a          451 c          771 g         1269 t
ORIGIN      
        1 ttctttaaat aaaaataatg tcaattataa agtaataaaa gtttagatat aaaaaaattt
       61 gtgtatatac tatatgtagt atataataga taaagcactg actatatgta gtgcttttgt
      121 ttttgaaaaa tacttattgc atttctataa ttatagatta attttgggat ttcaactgat
      181 aagtaccaaa agaaataata agcaaattaa gtgaaattat ggcacaagat tatgttaaaa
      241 ggacggtaaa taatgaattg gatagagctt aacaatcaag tgataattaa aaatgaacaa
      301 ggcaagtacc aattggaaaa ggataaggaa gccttaagta gttacataga agaatttata
      361 cagccaaagt tgaaaaaatt taataaccta gaagaaagat tgaagtattt aatagatgaa
      421 ggttattatt caaaagaagt aataaatcag tatagcatag atgttatcaa aaatatatat
      481 gatatgattg ataaacataa gtttgaattt caatcatata tgagtgcaaa taagttttat
      541 caaaattatg cattaaagag caatgatgga aaggaaatat tagaaacata taatgataaa
      601 gtgttaattg tagctttgac actaggaaat ggcaataaag atttagcttt aaacttggca
      661 gataaaatag taaaacaaga atttcaacca gctacaccta cttttttaaa tgctggaaga
      721 aaaagggctg gagaaatggt atcatgtttt ttactatctg tagaagatag tacagaaggt
      781 atatcgtatg ctatatcttc atcaaatcac ttatcaaaga ttggtggagg agttgcacta
      841 aatttatcta aacttagagc atctggcgaa tctatcaaag atatagaagg tgcagctgga
      901 ggtgttgttg gagtagctaa aatgttagaa caatcattta gttactttaa tcaaatggga
      961 gctagacaag gttctggtgc ggtgtacctt actgtattcc atcctgattt tgaattgttg
     1021 atggatacta aaaagataaa tgcagatgaa aaaataaggc ttgctacatt atcattagga
     1081 gctataatac cagataaatt tatggagttg gcagaaaaaa atgaagtagc atatgcattt
     1141 tatcctcata ctgtgtataa gaaatatgga gtttctcttg atgagataga aatggatgag
     1201 tggtatgata aattggttaa tgatagtgat ataagaaaaa aagaaataaa tcctaggcag
     1261 atgttgacaa aaatagcaca gatgcaacaa gaaagtggat atccatatgt agtgtacata
     1321 gatacagcta atagagaaca tacattaaaa gatgttggaa ttattaaaat gtctaactta
     1381 tgttgtgaga tatttcaata tcaaacacct tcagaaatag aggggtatgg aggaaaaaat
     1441 gaatggggac aagatataag ttgtaaccta ggttctttaa acatagccaa tgtaatggat
     1501 aataaaacta tagaaagtac agttgaaaca gcaataagag cactgtcatt tgtagcagat
     1561 agcacagata taaaaccagt accaacggta agcaatagta acagcaagtc tcattctata
     1621 ggattaggag ctatgaattt acatggatac ttagtaagag aaaatatttt atatacttca
     1681 gatgatgcta tagatttttc taatgtattt tttgcaatgg ttagatacta ttcaattaaa
     1741 gcttctatga aaatagctat agaaagaaat caaacttttg aaggatttga taaatcagaa
     1801 tatgttaagg gaagaaatag caaggtttta tcaaaatact atgaacaatc ttatctacct
     1861 aagtcagaaa aggtaagagc attatttgaa gggatttata taccaacaaa agaagactgg
     1921 acaaaattat tggatgaggt aaaagaaaaa gggatatata atgcatattt aatggcagta
     1981 gcaccaactc aaagcattag ttatgttcaa aatgctacat caagtataat gccaattact
     2041 gagccagtag aagtaagaac atatggagac tctactacaa tatatccaat gccattttta
     2101 acaaatgata atatgttgta ttatcaatca gcatacagga tggatatgag aaaggtcata
     2161 gatttagtgg caacagttca aaaccatgtt gaccaaggta tatcaacaac actgtttgtt
     2221 acagatgaaa agacaactag agatatagct agacattaca tatatgcata taaaaaaggt
     2281 ttgaaaagtt tatattacac aagaacaaag atgacaagag atactcatga gtgtctagtg
     2341 tgttcagtat agggagagaa atatatgaca tttaatttac ataaaataca taatgcagtt
     2401 aactggaata gagaagaaga tggatttaca caagcttttt gggaacaaaa tgtaaaacaa
     2461 ttttggcttc ctgaagaaat atctgtttct aaagacataa aggtatggaa tgaactaagt
     2521 aataaagaaa aggaattata taaaaaagta ttgggtggtt taacattact ggatacaaaa
     2581 caggggaata atggaatacc atctatgatg agtttaacag aaaacctaca aagaaaagct
     2641 gtcttgtctt ttatggggac tatggaagaa atacatgcaa aaagttattc ttctatattt
     2701 atgactctac tttctaattt agaaatagat gaattatttg aatggataga aacagaacca
     2761 acattacaaa gaaaggctga tttagtttta gctcaatacg aaaatactac aaatcaagag
     2821 ggattatacc tatcaatggt tacaagtgta tttttagaga gtttcttgtt ttattcagga
     2881 ttcttttatc cattgtatct atctggtcaa ggaaagatgg tagctagtgg agaaataata
     2941 tcgcttattc ttagagatga gtctttacat ggtaagtata taggattgct agcacaagaa
     3001 atatacgatt catttgataa aatggataag gagatgttag aagagaagat gtattctata
     3061 ctttatagcc ttatggagaa tgagatagag tatacaaatg ttatatacag agaaagtggg
     3121 cttgaaaaag aggttgtaaa ctttttaaaa tacaatgcga atagagcttt agaaaatcta
     3181 gggtttgaga agctatatac tgtagatgct ataaatccaa ttgtattaaa tggtcttagc
     3241 actgaaacca aaacacatga tttcttttca actaagggta atggatacca aaaaggagtg
     3301 tatgaggaat tagaggatga agattttata atataattta taattatgac acactgttgt
     3361 cacttttagc gtgataatat caatgtataa aatatatatg gaggtatttt tatgaaatat
     3421 gaatggagaa aaagagataa agagttatat ttacctaaaa agaatcctgt aatagttgaa
     3481 ataccagaac agaaatttat catgttaaaa gggagtggag acccaaattc taaagaattt
     3541 actgaagcaa taggagtact ttattctttg gcatatgcaa taaaaaccat gcctaaaaaa
     3601 ggaattgtac cagaagggta ttttgactat acggtttttc ctttagaagg tatatgggat
     3661 aagggagaaa aaagtaaagg cttagatgta cttattaaag aagatttaat ttacactata
     3721 atgatacgtc agccagattt tgttacacaa gacctgttgg ataaagctac tgatatagta
     3781 aagaagaaaa aacctcataa tcttcttgaa aaagttagat ttgggtctat taatgaaggg
     3841 ctatgtgttc aaaagcttca tataggttca tatgataatg aaccagaaag ctttgacatt
     3901 atgaacgaat tttgcaaaga aaataaactt ataagaaaat cttacacaca tcgtgaaatt
     3961 tatataagtg atgctagaaa agtatcgcct gaatcattga agacggtttt acgttttgaa
     4021 gtagaaaggg aagtataaat attttgaata atttataaga gttataaaaa gtgaagtctg
     4081 ttgagacttc attttttgat gcaattttaa aattaaattt gatgtttcat aatattttaa
     4141 aaactttcta cttttattag t
//
LOCUS       DAELLV010000274         4143 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.274, whole genome shotgun sequence.
ACCESSION   DAELLV010000274 DAELLV010000000
VERSION     DAELLV010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4143
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.274"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            372..2948
                     /locus_tag="KPD58_003329"
     CDS             372..2948
                     /locus_tag="KPD58_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG0267058.1"
                     /translation="MKDNLKIVLRYIKSYKARSLAIVVSIILATSLVVGIGTLSRSAQ
                     QADVDRLKRELGTDHVYFKNIKKNQLKSIKSSKDIKNLGITSYYASTDVNEKLPINIL
                     YTNKNYLTSHSKLIKGRFPKASNEVVVEKWILNSMGLKPEINQNLTFKLYQKENPETF
                     KVVGILEDRFIEKKKGQCEIFSNLNESELNDFTTYIEFNEGSDINTNIDNIIKNAMLD
                     EKSIGRNSMLIESTMKNGTLDNSSKYVAIAMSLFAGIVIYSIYVISIYQRVQEYGVLR
                     AIGSTNFRIFKLMLYELFILALIAMPIGICIGMGGAQIFNRSVGNIQFEGNINVTPFV
                     IPDKIILLSIACTILIILIISFFTYLKIRRISPIDAIRKTFGADKNVTKVNYLISKLT
                     LNMSVTKSISAKNIFRNKKGFIIIVLSMSIGGIIVIKDNYKYSFLDVQNKSTQEQTYM
                     NADFILSNVYLNEIGNNTSFKDIKGFNDNQIDKIKNIDGIDKVKTASILNTRIEVNKI
                     NRLDYYEAINSTPYYKLNPLFIKNKTTGKYTLKQKLKGYNDEMIKSLEKYLVSGNINL
                     KKMKKENLAIVYVPEISKTQKYTKTFTPGEGTPAVDIKVGDTIKVKYPKGGMDTGLYE
                     SLKDNYEYLEYEFKVGAIISYPFADNGMYSGDDGVDVITSSEYLKKLTGVDKYNLVYA
                     DLNENANKKKINNLLGKIGSEVPGTTTVDMMVEKENSDKMTSRTMIYSYGIVAIMFII
                     SVLNIINNISYNLTSRTSEFGMLRAIGISERGFKNMILYEGILYGVLSSIITIVSGII
                     IQFKMYYMQGFVSYGLGFSIDYKIYILVVVANIIVGILATYIPSRKINKISIVEAINI
                     TE"
     gene            2960..3631
                     /locus_tag="KPD58_003330"
     CDS             2960..3631
                     /locus_tag="KPD58_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0267059.1"
                     /translation="MPILETINLGKIYGKKETSVHALKNANLKINKGEFVAIIGPSGS
                     GKSTFLHLVGGLERPSNGTIKVDGKDICCLSDKELARYRRQKVGFVFQQYNLIPVLNV
                     KENIELPLKLDNKKIDKEYIDDLINLLGLNERKNHLPNQLSGGQQQRVAIARALSAKP
                     SIILADEPTGNLDSKTTEEVMDLLKSSIKKYNQTLIIITHNENIARKADRIISIIDGE
                     LKLTL"
     gene            3658..3999
                     /locus_tag="KPD58_003331"
     CDS             3658..3999
                     /locus_tag="KPD58_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0267060.1"
                     /translation="MLLILGNLNLNLDFDYRIIREENEDVDIFIDINYRSLDIDTDGS
                     NLFNSRIQFPFVRALILRLNKNNQCMTIHLLRDIDLFSAFANFEVDYTDSIINIKNQN
                     EKVILNKSIKK"
BASE COUNT         1715 a          462 c          628 g         1338 t
ORIGIN      
        1 gtgccctcca taaataaaat caatgcaaaa cacgatataa aaaatgttaa tatgcctctt
       61 tttaatatgt ctaattcttt gtaaaattta tacttattca ttataagatt gctaaatata
      121 aacaaagtta gtattactat aggaaataca aaattgttat ccatgattcc cctcccctct
      181 acaatttata tagggattat atcattttaa aaaaattatg taaaaagtta aaatttacat
      241 atatattaat taaacttgta aaaatacaga tgaatttgta caattaacta cttaaaattt
      301 gaaaatactt actttgctat atattttaga taaatatata attgatttag ttaagaaagg
      361 aagtggtaaa tatgaaagat aatttgaaaa tagtactaag gtatataaaa agttataaag
      421 ctaggtcttt agcaattgtt gtgagtataa ttcttgctac atcactcgtt gttggtattg
      481 gtactctctc aagaagtgct cagcaagcag atgtagatag attgaaaaga gagcttggaa
      541 ctgaccatgt atactttaaa aatataaaga aaaatcaatt aaagtccata aaatctagca
      601 aagatattaa aaatctgggt ataacatctt actatgcgtc tacagatgtt aatgaaaaat
      661 tacctataaa catactatat acaaataaaa attatttgac tagtcattct aaacttataa
      721 aaggtcgatt tccaaaagct agcaatgagg tggtagtcga gaaatggatt ttaaatagta
      781 tgggcttaaa accagagata aatcaaaacc ttacttttaa attatatcaa aaagaaaatc
      841 cagaaacttt taaagttgtt ggaatccttg aagatagatt tatagagaaa aaaaagggac
      901 aatgtgaaat tttttctaat ctcaatgaat cagaactgaa tgattttaca acttatattg
      961 aatttaatga aggcagtgat ataaatacaa acatagacaa tataataaaa aatgctatgt
     1021 tagatgagaa aagtatagga agaaactcaa tgcttataga atctactatg aaaaacggta
     1081 ctttagataa ttcaagtaaa tacgttgcaa ttgctatgag tctatttgct ggtattgtaa
     1141 tatatagtat ctatgttata tcaatatacc aaagagtaca ggaatatggc gtcttaagag
     1201 ctattggttc caccaacttt aggattttca aacttatgct ttatgaactt ttcatacttg
     1261 ctctaatagc aatgcctata ggtatatgta taggtatggg aggtgcacaa atatttaata
     1321 ggtctgtagg aaatattcaa tttgaaggaa atataaatgt tacacctttt gttattccag
     1381 ataaaataat tctcttatca attgcatgta caatactaat aatactaatt ataagtttct
     1441 ttacatactt aaaaataaga agaatttctc ctattgatgc tatcagaaaa acttttggag
     1501 ctgataaaaa tgtaaccaaa gttaattatt taatatcaaa attaacttta aatatgtcag
     1561 taacaaaatc tatatctgcg aaaaacatct ttagaaataa aaaggggttc attataattg
     1621 tattatctat gagtatagga ggtataatcg tcataaaaga caattataaa tattcttttt
     1681 tagatgtgca aaacaaatct actcaagaac aaacatatat gaatgcagat tttattctat
     1741 caaatgttta tctcaatgaa attggtaaca atacttcttt taaagatata aaagggttta
     1801 atgataatca gatagataaa ataaaaaata tagatggaat tgacaaagtc aaaactgcta
     1861 gtatcttaaa cacaagaata gaggtaaata aaataaatag attagattac tacgaagcta
     1921 taaattcaac tccttattat aagctaaatc ctctgttcat aaaaaataag actactggca
     1981 aatatacact aaagcaaaag ctaaaaggtt ataatgatga gatgataaag tcattagaaa
     2041 aatacttagt aagtggaaat ataaacttga aaaaaatgaa aaaagaaaat ttagctattg
     2101 tatatgtacc agaaatttct aaaacacaga aatacacaaa gacttttact cctggtgaag
     2161 gaacaccagc tgtagatata aaagtaggtg atactataaa agtcaaatat ccaaagggtg
     2221 ggatggatac aggtctttat gaaagtctaa aagataatta tgaatactta gaatatgaat
     2281 ttaaagttgg agctattata agttatcctt ttgcagataa tggtatgtac tctggagatg
     2341 atggtgtaga tgtaataacg agtagcgaat acctaaaaaa attaactggt gtagataagt
     2401 ataacttagt ttatgcagac cttaatgaaa atgcaaacaa gaaaaaaata aataaccttt
     2461 taggtaaaat tggaagtgaa gttcctggaa ctacaacagt agatatgatg gtagaaaaag
     2521 aaaattctga taagatgaca tctagaacta tgatttatag ttatggtata gtagcaataa
     2581 tgttcataat cagtgtactt aacataataa acaacataag ttataactta acttcaagaa
     2641 ctagtgaatt tggaatgtta agagctattg gtatctctga gagaggcttt aaaaatatga
     2701 tactttatga aggaatatta tatggtgtac tctcaagtat aattactatc gtgtcaggaa
     2761 ttataataca atttaaaatg tattatatgc agggctttgt atcatatgga ttgggatttt
     2821 caatagatta caaaatatat atactagttg ttgtagcaaa tataattgta ggtattctag
     2881 ctacatatat tccatcaaga aagataaata aaattagtat agttgaagct ataaacatta
     2941 cagaatagga ggaataatta tgcctatatt ggaaactatt aacttaggta agatttatgg
     3001 aaaaaaggaa acatctgtac atgcactaaa gaatgcaaat ttaaaaataa ataaaggcga
     3061 atttgttgct ataattggac caagtggaag tgggaaaagt acatttctac atttagtagg
     3121 tggtcttgaa agaccaagta atggtactat aaaggtagat ggtaaagata tatgttgtct
     3181 ttcagataaa gaattagcaa gatatagaag acaaaaggta ggatttgtct ttcaacagta
     3241 taacttaatt cctgtattaa atgtaaagga aaatatagaa ttaccactaa agttagacaa
     3301 taaaaaaata gataaagaat atattgatga ccttataaat ttattgggtc ttaatgaaag
     3361 gaaaaatcat cttcccaacc aattatctgg tggtcaacag caaagagttg ctattgcacg
     3421 tgctttatct gcaaaaccaa gtataatcct agcagatgaa ccaacaggaa atttggacag
     3481 taaaactaca gaagaagtca tggatttact aaaaagttct ataaaaaaat ataaccaaac
     3541 acttataata ataacacata atgaaaatat tgctagaaaa gcagatagaa ttatatctat
     3601 aatagatgga gagcttaaat taacactcta aaactattta tagaaaagag gatttaaatg
     3661 ttattgattt taggtaattt aaatttaaac ttggattttg actacagaat aataagagaa
     3721 gaaaatgaag atgtagatat atttatagat attaattatc gtagcttaga tatagatact
     3781 gatggttcta acttatttaa ttctagaata caatttccat ttgtaagagc attgattcta
     3841 aggctaaata agaacaatca atgtatgaca attcatttgt taagagatat agatttattc
     3901 tctgcttttg ccaactttga agtggattat actgactcta taataaatat aaaaaatcaa
     3961 aatgagaaag taattttaaa taaatctatc aaaaaatagt aagggggcct taataggccc
     4021 caattttttt cttttctaaa cttattatat catgataaat aaattagtca atagaatttt
     4081 ctgaatattt ttataatttt ttcataaagt tttttatata ttatgcttta aaattatatg
     4141 taa
//
LOCUS       DAELLV010000275         4077 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.275, whole genome shotgun sequence.
ACCESSION   DAELLV010000275 DAELLV010000000
VERSION     DAELLV010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.275"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            120..575
                     /locus_tag="KPD58_003332"
     CDS             120..575
                     /locus_tag="KPD58_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HBG0267061.1"
                     /translation="MESSFYMKEALKEAYKAYNKKETPIGAIIVKDNQIIARAHNLTE
                     TLKDSTAHAEILAIKQASEKLGGWRLTDCDLYVTMEPCIMCSGAIVNSRIKKLIIGTR
                     HVKNSYIEKQHEFKLDYFNNNNVKVAFDVLQEECSIILQEFFKALRKRD"
     gene            576..667
                     /locus_tag="KPD58_003333"
     tRNA            576..667
                     /locus_tag="KPD58_003333"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:610..612,aa:Ser,seq:gga)
     gene            705..969
                     /gene="ffs"
                     /locus_tag="KPD58_003334"
     ncRNA           705..969
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="KPD58_003334"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            1046..2683
                     /gene="dnaX"
                     /locus_tag="KPD58_003335"
     CDS             1046..2683
                     /gene="dnaX"
                     /locus_tag="KPD58_003335"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBG0267062.1"
                     /translation="MHKALYRAYRPQKFEDVIGQDHIIKTLKNQIYSDNIGHAYLFCG
                     TRGTGKTSTAKIFSRAVNCLNKINEEPCNECEICESVLKDNTMDVVEIDAASNNSVDD
                     IRELRESVKYSPANAKYKVYIIDEVHMLSQGAFNALLKTLEEPPSYVIFILATTEPHK
                     IPATILSRCQRYDFKRVTVKDMTLRMKKICEDEGIDIDDKALNLIARNSQGALRDALS
                     ILDQCMSFGESKIDYKDVVELMGSVNIEQLFELSQCIVEQDTKKSLEILNEFVLWGKD
                     IRNLINDLIDHFRNLMVCKVSSELDEIISLPEETIEQLKIQSKNIDINDLIRILNILS
                     ITQDDIKSSSNPRVLVEITIMKIAQPMFDESKEALIKRVENLEKMIELGNFKSEKIGN
                     NKEKEYEVDREIDVKQENVVYEDVKNEDVILIESSWKNILKQIKKDKKMPIYALLSEV
                     KSFNVYSNMLYVIFDDKFDFAKTRLSSQDTINYLEKTIRDVLNRSFNVKIVLTSEVKD
                     INLEVKEKKDIGEEILKNIVSEEILEIKDSIDENESK"
     gene            2742..3092
                     /locus_tag="KPD58_003336"
     CDS             2742..3092
                     /locus_tag="KPD58_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBG0267063.1"
                     /translation="MAKKGFGGGMMPGGGNMNNLLKQAQKMQENMQKAQQELESKEVE
                     ASVGGGAVTVKVNGKKEVIDITIKPEVVDPDDIEMLQDLVLSAVNQALRNIDDIQASQ
                     MSKVTGGMNIPGLF"
     gene            3105..3704
                     /gene="recR"
                     /locus_tag="KPD58_003337"
     CDS             3105..3704
                     /gene="recR"
                     /locus_tag="KPD58_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBG0267064.1"
                     /translation="MQVYTGPITRLIEEFSKLPGVGRKTAQRLAFHIINMNTNDVEAL
                     SKAIIDAKREIRYCSICCNITDTDPCSMCSNKSRDSSVICVVEDPRDVAAMERTREFK
                     GQYHVLNGVISPMDGIGPDMLKIKELIQRLGNQEVKEIIMATNPTIEGEATAMYIARL
                     VKPMGIKVTRIAHGLPVGGDLEYADEVTISKALEGRREI"
     gene            3845..4066
                     /locus_tag="KPD58_003338"
     CDS             3845..4066
                     /locus_tag="KPD58_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0267065.1"
                     /translation="MKPIMNLEEHNAMKEKRVKKMKSKDKPKYDDFEDISFSNKKKTK
                     KNVRNKNAEFRNNYKNYQYDYEEDFYEPI"
BASE COUNT         1654 a          426 c          796 g         1201 t
ORIGIN      
        1 aactaatttt aaaaaaatat agctatatat agtaataatt aaataaacaa aataaaatta
       61 agttgaatta taaaatgaat tatgataaga taagtttgtt ataagttttg ggaggaatta
      121 tggaaagttc attttatatg aaagaagcat taaaagaagc atataaagca tataataaaa
      181 aagaaacacc aataggtgct attatagtta aagataatca aataatagct agagctcata
      241 accttacaga aacgttaaaa gactctacgg cacatgcaga aattttagcg ataaaacaag
      301 catcagaaaa attaggtggt tggaggttga ctgattgtga tttatatgtt acaatggaac
      361 cttgcataat gtgtagtggc gcaatagtta attctagaat aaaaaagctt ataataggga
      421 caagacatgt aaaaaattca tatatagaaa aacagcatga gtttaaatta gattatttta
      481 ataataataa tgtaaaagta gcttttgatg ttttgcaaga agaatgctcg atcatattgc
      541 aagaattttt taaggcattg agaaaaagag attaaggaga gatggtcgag tggtcgaagg
      601 cgctcgcctg gaaagcgagt atatgggtaa aactgtatcg tgggttcaaa tcccactctc
      661 tccgccagaa acaatgaaag taattaaata tggttgtaat tttgctgtac tagatgggga
      721 ggtagcggtg ccctgtaacc tgcaatccgc tatagcagga ttgaattcca tctaaaggct
      781 tattttctgt agggccgccc taaataagtg gtgttgatgc ttaggtcctg cgcaatgaaa
      841 gctcatgaac cccgtcagat ccggaaggaa gcagcggtaa gtgatcactt ttgtgtgccg
      901 tgggggtgcc taagttgagc taactgtttt ggtaacgcct atggaattaa gtcgatagat
      961 ggtgtacagc atttaatata taaaagacta atcccttaga taatgaggga tttttttatt
     1021 aattttgcta aagagaggta aaaagatgca taaagcatta tatagagcat atagaccaca
     1081 aaaatttgaa gatgttattg gtcaagatca tattattaag actttaaaga atcaaatata
     1141 tagtgataat ataggacatg cttatttgtt ttgtggaaca agaggtactg gtaaaacatc
     1201 tactgcaaag attttttcaa gagcagtaaa ctgtttgaat aaaataaatg aagaaccgtg
     1261 taatgagtgt gagatatgcg aatctgtatt aaaagataat acaatggatg ttgtagaaat
     1321 agatgcagct tcaaataata gtgtagatga tataagagag cttagagaaa gtgtgaaata
     1381 ttctccagcc aatgctaaat ataaagtata tataatagat gaagttcata tgttgtctca
     1441 aggagctttt aatgcattat taaagacctt agaggaacct ccatcatatg taatttttat
     1501 attagcaact acagagcctc acaaaatacc tgcaaccatt ttatcaagat gccaaagata
     1561 cgattttaaa agagttacag ttaaagatat gacccttaga atgaaaaaga tttgtgaaga
     1621 tgaaggaatt gatatagatg ataaagctct taatttaata gctagaaatt ctcaaggagc
     1681 tcttagagat gcacttagta tattagacca atgtatgtct tttggagaaa gtaagataga
     1741 ttataaagat gttgttgaat taatgggaag cgtaaatata gaacaattat ttgaattgtc
     1801 tcaatgtata gtagagcagg acactaaaaa gtctcttgag atattaaatg aatttgtctt
     1861 atggggcaaa gatataagaa atcttataaa tgacttaatt gatcatttta gaaatttaat
     1921 ggtatgtaag gtttcatcag aattggatga aataatttca ttaccagaag aaactataga
     1981 acaacttaaa attcagtcta aaaatataga tataaatgat ttaataagga ttttaaatat
     2041 attatcaata actcaggatg atataaagtc atcttcaaat ccaagggttt tagtggaaat
     2101 aaccataatg aaaattgctc aaccaatgtt tgatgaaagt aaagaagctc ttataaaaag
     2161 agttgagaac ttagaaaaaa tgattgaact ggggaacttt aagtctgaaa aaattgggaa
     2221 taataaagaa aaagagtatg aggtcgatag agaaatagat gtaaaacaag aaaatgtagt
     2281 ttatgaagat gtaaaaaatg aggacgttat acttatagag tcatcttgga agaatatact
     2341 taaacaaatt aaaaaagata aaaaaatgcc aatatacgca ctcttaagtg aagtaaaaag
     2401 ttttaatgta tattctaata tgttgtatgt tatatttgat gataaatttg attttgcaaa
     2461 aactagactg agctctcaag atacaataaa ttatttagaa aaaacaataa gagatgtttt
     2521 aaatagaagt tttaatgtaa aaatagtact gacatcagaa gttaaagata taaatcttga
     2581 agttaaagag aaaaaggaca ttggtgagga aatattaaag aacatagtga gtgaagagat
     2641 attagaaata aaagatagta ttgatgaaaa tgagagtaaa taataaaata ctattatgat
     2701 ataataataa aatgaaaata agtttaggag gaatagtgtt tatggctaaa aaaggttttg
     2761 gaggcggaat gatgcctggt ggtggaaata tgaacaactt gttaaagcag gctcaaaaaa
     2821 tgcaagaaaa tatgcaaaaa gctcaacaag agttagaaag taaagaagta gaagcttcag
     2881 ttggtggagg agcagttact gtaaaagtta atggtaaaaa agaagttata gatataacta
     2941 tcaagccaga agtagttgat ccagatgata tagaaatgct acaagattta gttttaagtg
     3001 cagtaaatca agcattgaga aatattgatg atatacaagc aagtcaaatg agtaaggtaa
     3061 ctggaggtat gaatatacct gggttattct agtaggtggt atttatgcaa gtttatacag
     3121 gtcccataac gagacttata gaggaatttt caaagcttcc tggagtagga agaaagactg
     3181 ctcaaagatt ggcttttcat attattaata tgaatactaa tgatgtggaa gctttatcta
     3241 aagcgattat agatgcaaaa agagaaataa gatattgctc aatttgttgt aatataacag
     3301 atacagaccc atgtagcatg tgttcaaata aaagtagaga ttcaagtgtt atatgtgttg
     3361 tggaagatcc aagagatgta gcagctatgg agagaacgag agagtttaaa ggacagtacc
     3421 atgtattaaa tggagtaatt tctccaatgg atggaattgg accagatatg ttaaaaataa
     3481 aggaacttat ccaaagactt ggcaatcagg aagtcaaaga aataataatg gcaacaaatc
     3541 ctactattga aggtgaagct acagccatgt atattgctag gttagtaaaa ccaatgggaa
     3601 tcaaagtcac tcggatagct catggtcttc ctgttggagg cgatttagag tatgcagatg
     3661 aggtcacaat atcaaaagca ctagaaggca gaagagaaat ataaaaatcg aggttaatac
     3721 ctcgattttt gtaatttttt gagttaaatt caaattaatt ttgatttaga tttattgaat
     3781 aaatttcttc tgtattgtat aatttgttgt ataaatcatt taaaaatttt gataggggtg
     3841 caacatgaaa ccaattatga acttagaaga acataatgca atgaaagaaa aaagagttaa
     3901 aaaaatgaaa tcaaaagata aacctaaata tgatgatttt gaggatataa gtttttctaa
     3961 taagaaaaaa actaaaaaaa atgtacgaaa taaaaatgct gaatttagaa ataattataa
     4021 aaattatcag tatgattatg aagaagactt ttatgaacca atttaatatt aaataat
//
LOCUS       DAELLV010000276         4072 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.276, whole genome shotgun sequence.
ACCESSION   DAELLV010000276 DAELLV010000000
VERSION     DAELLV010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.276"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(448..693)
                     /locus_tag="KPD58_003339"
     CDS             complement(448..693)
                     /locus_tag="KPD58_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HBG0267066.1"
                     /translation="MLIHTKNKTFDAICILEKIEKDLNIEELVRCHKSYIINLNYVEN
                     IKSNTAILDSQEEVPISRYRYKDIKERFLKFIGDRIC"
     gene            complement(1010..1162)
                     /locus_tag="KPD58_003340"
     CDS             complement(1010..1162)
                     /locus_tag="KPD58_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0267067.1"
                     /translation="MKNLNKNTILGEVIITIAACDDEKEIQIVIKNQVDNILKNKNKE
                     YRIKKI"
     gene            1771..3237
                     /gene="dndC"
                     /locus_tag="KPD58_003341"
     CDS             1771..3237
                     /gene="dndC"
                     /locus_tag="KPD58_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA phosphorothioation system sulfurtransferase
                     DndC"
                     /protein_id="HBG0267068.1"
                     /translation="MAVNYLDNNNLENLYDEMQAVYLNDDRPWIIGYSSGKDSSCVVE
                     LTFRMLKRLPKEKRHKEIYVISSDTLIENPIILDYLKNNIDKINESATEHDLPISAQI
                     VYPELNDSFWANIIGRGYPTPKSIKYRWCTERLKIKPSNKFIKEKLEQKDVIVLLGVR
                     KEESSARKSRIEKREIEGYLLTPHETLRGKNKLAYVYTPIVNFSTADVWDVLYHQNDN
                     FIDFGGEFGPSPSTSWGTDAMELFQMYMKGSGDSDECPFIADEASAKSSCGNSRFGCW
                     ICTVVKEDKSLNGFIESGHDELKPLLEFRSWLISERDKPKNRKKHKRNGSVIKKDGRI
                     MFGPFTFEARQTILRKLLETQLEMQKFYPDLQLIQLGELKAIDDIWDNEEDLTGNILS
                     KIYKEVVGKNLPWSEYKKPVFDDLTLNIISEKCSEYDINIDLFNKLMIDTNKYKYFSN
                     NTKLKTSIERILNQQWLHQDIIEKIEEESNKELKYENK"
     gene            3224..>4072
                     /locus_tag="KPD58_003342"
     CDS             3224..>4072
                     /locus_tag="KPD58_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0267069.1"
                     /translation="MKINKLVLKNFRSYEEETTFNLNTTSEKNIILIGGKNGAGKSTI
                     FEAIKLCIYGPIAYKYQGFNSSYISRVKSNINNNSLRNSKVDTLVSVDIEISEGTEKN
                     TYTLIRQWTFKDKKLNETFSVYKNFSFTPLNADELNYFENYITSIISPKIFDFFFFDG
                     EHLYDFFIGKNSNIHLKESLLSLCNFDTFDILKKMLISTNRSNKNVSDEINIIKDDYL
                     NLEVEINNLNSQEKNLSYELEKISSEIEKLQQEQLKIKKEFRKKGGILAEEREMLNSR
                     IAELEGN"
BASE COUNT         1515 a          457 c          560 g         1540 t
ORIGIN      
        1 attattataa gataatagta tatatctact ttagcattat cataactaaa cactaataga
       61 gcttcctctg atgatattac attacttgta actacaaaaa ataaatttat agatataaca
      121 tcttttatta aagggtgata tacgtcaaat aagaaaaatg aaactatata tttaacaatt
      181 cctatcttat aaaaataatt atagtaagtt cccaagtaat aaatatcaaa taatagatta
      241 tatcatatag atttttcttc atattataat catgggtctt atcaacgtta aataatataa
      301 tgattttagt tataagataa gctaataata cagtaaaaaa agatattttc ttagtcttat
      361 ctgtatcttt atcgtaagtt aatatacttt ttagtatcat tattggtgta aacaatgata
      421 atattaataa tattttattt atatggttta gcatattcta tcaccaataa attttagaaa
      481 cctctctttt atatctttat atctatatct gctaataggt acttcttctt gactgtctaa
      541 tattgcagta tttgacttta tattttctac atagtttaag ttaattatat aacttttatg
      601 acatcttaca agttcctcta tatttaaatc tttttctatt ttttctagta tacatatagc
      661 atcaaaagtt ttattttttg tatgtattag catgttcttt ttttgaactt ctatctattt
      721 ttttgctaga gtagattttg tatgtgttag acttattttt atttttttca atttttttga
      781 tacatgttaa tatgtgtttt tctagttctt gaaaatttat tggctttaat aagtatcaat
      841 aagctcttac ttcatatctt tcctgtacat aatttattag agatgttata aatattatta
      901 tctacttttc ttatgtttct tgcgatatct atactattta gttctggcat ttttatatct
      961 aagagtagtg tatcctctca attaactttg taatggttct tcttttgagc tatatttttt
     1021 taattcgata ttctttgttt ttatttttta atatattatc aacttgattt tttattacga
     1081 tttgtatttc tttttcatca tcacaagctg ctattgtaat gatgacctct cctagtattg
     1141 tatttttatt tagattcttc actttaggtt aacacttttt aaatgtattt atcaataata
     1201 tttaaattaa aaatgtataa aaatacctat atccaattgg atataggtat tcgtggattt
     1261 caatggtgga tacgagggga gtctataagt ggttatttga ttgttttgtt ggtttttgtg
     1321 aatgtaggta tatagctatt tagttatgat attagactga tttttttggt tattttttat
     1381 tgtttatttt tgtggttggg gtattattgg ggtataactt ttaaagttta taaattgagt
     1441 cattaataaa tatctattgt ttagtcaatc tataatataa taaatcttaa atagtgtaac
     1501 tattctaagt agctgctatt tttattcata catatcttta tatacattta tgagattcaa
     1561 gtatgtacat tcttattctc taatttttaa atatattatc ttaaaatggt aacagttcta
     1621 catttaatta aatttatatt ttattgtact tatgtaaata taaactaatc tattctctaa
     1681 atatatattg tgatataatt atatataaat taaaatagga gtattatgta aagatcttcg
     1741 ctatactctt atagaaagga tacttaatat atggctgtta attacctaga taacaacaat
     1801 ttagaaaatt tatatgatga aatgcaggcc gtatatctta atgatgatag accttggata
     1861 atcggatata gttctggaaa agatagttct tgtgtagttg aacttacatt tagaatgctt
     1921 aaaagactac ccaaagaaaa aagacataaa gagatttatg taatctcatc tgatacactt
     1981 atagaaaatc caataatact tgattatcta aaaaataata tagacaagat aaatgaaagt
     2041 gctactgaac atgatttacc tataagtgca cagattgtat acccagaact taatgatagt
     2101 ttttgggcca atataattgg tagaggttac cctactccaa agtctattaa atatcgttgg
     2161 tgtactgaaa ggcttaagat aaaaccatca aataaattta taaaagaaaa acttgaacaa
     2221 aaagatgtta ttgtactgct tggcgttcgt aaagaagaaa gttctgctag aaaatcgaga
     2281 atcgaaaaaa gagagataga gggttacctt cttaccccac atgaaacctt aagaggtaaa
     2341 aataagttag cttatgtgta tacccctata gtcaatttta gtacagcaga tgtttgggat
     2401 gtactctatc atcaaaatga taatttcata gattttggtg gtgaatttgg accatctcca
     2461 tctacctcat ggggaactga cgctatggaa ttatttcaga tgtatatgaa aggttctggt
     2521 gactcagatg aatgcccatt tatagcagat gaagctagtg ctaaaagtag ctgtggaaat
     2581 agtaggtttg gatgttggat ttgtactgtt gtaaaagaag acaaatcatt aaatggattt
     2641 attgaatctg gtcatgacga attaaagcca cttttggaat tcagaagttg gctaatttct
     2701 gagagagata aacctaaaaa tcgtaaaaaa cataaaagaa atggctctgt catcaaaaaa
     2761 gatggtagaa tcatgtttgg accatttaca tttgaagcaa gacaaactat tttaagaaaa
     2821 cttttagaaa ctcaacttga aatgcaaaaa ttttatcctg atttacaact tattcaatta
     2881 ggagaactta aagctataga tgatatttgg gataacgaag aagacttaac tggtaatata
     2941 ttatcaaaaa tatataagga agttgtaggc aaaaacctac catggtctga atataaaaaa
     3001 ccagtatttg atgaccttac tttaaatatt atatctgaaa aatgtagtga gtatgatata
     3061 aatattgatt tatttaataa attaatgata gatacaaata aatacaaata ttttagtaat
     3121 aatactaaat taaaaacttc catagaaaga atattaaatc aacagtggct tcatcaagat
     3181 attattgaaa agattgaaga agaaagcaac aaggagttaa aatatgaaaa taaataaact
     3241 tgtattgaaa aatttcagat cttatgagga agaaaccacc ttcaatctaa atactactag
     3301 tgaaaaaaat attatattga taggtggtaa aaatggtgct ggtaaatcca caatatttga
     3361 ggctataaag ctatgtatat atggacctat agcttataaa tatcaaggtt tcaactcttc
     3421 atatatatct cgagttaaat ctaatataaa taataattct ctaagaaata gtaaggttga
     3481 caccttagta tcagtagata tagaaatcag tgaaggtact gaaaaaaata cctatacctt
     3541 aattagacaa tggactttta aagataaaaa attaaatgaa acattttcag tttataaaaa
     3601 tttttcattt acaccattaa atgctgatga acttaattat tttgagaatt atattacatc
     3661 tattatttct cctaaaatat ttgacttttt cttttttgat ggagaacatt tgtatgattt
     3721 ttttataggt aaaaattcaa atatacatct aaaagaatct ttattatctc tttgtaattt
     3781 tgatacattt gatattttaa aaaaaatgtt aatttcaact aacagatcca ataagaatgt
     3841 ttcagatgag attaatatta taaaagatga ctatttaaat ttagaagttg aaatcaacaa
     3901 cttaaactct caagaaaaaa atctatctta tgaattagaa aaaatttcaa gtgaaattga
     3961 gaagttacaa caagaacaat taaaaataaa gaaagaattc aggaaaaaag gtggaatttt
     4021 agcagaagaa agagaaatgc ttaattctag aattgctgaa ttagaaggaa at
//
LOCUS       DAELLV010000277         4056 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.277, whole genome shotgun sequence.
ACCESSION   DAELLV010000277 DAELLV010000000
VERSION     DAELLV010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4056
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.277"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            419..1312
                     /locus_tag="KPD58_003343"
     CDS             419..1312
                     /locus_tag="KPD58_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HBG0267070.1"
                     /translation="MSNKIVSKRQLTDSIYLMEIEAPRVAKSSQPGQFIIIKNDEKGE
                     RIPLTIADYDREKGTVTIVFQTVGASTKKLAMFEENDFVMDFVGPLGQASEFIHEDIE
                     ELRNKKILFVAGGVGSAPVYPQVKWFKEHGLDVDVIIGARTKELIILEDDMKKVAKNV
                     YVSTDDGTYGFNGRVTDLLKDLVDNQGKKYDQAIVIGPMIMMKFMCQLTKELNIPTIV
                     SLNTIMIDGTGMCGGCRVSVGNETKFACVDGPEFDGHLVDFDQAMRRQSMYKTQEGRA
                     MLKLEEGDSHHHSNCGCGGNK"
     gene            1312..2706
                     /gene="gltA"
                     /locus_tag="KPD58_003344"
     CDS             1312..2706
                     /gene="gltA"
                     /locus_tag="KPD58_003344"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HBG0267071.1"
                     /translation="MDAKKVKVPVREQEPAVRATNFDEVCLGYNKEEAMAEANRCLAC
                     KKPKCVGGCPVGIDIPGFITKIKEDDIEGAAKVIAKSSSLPAVCGRVCPQESQCEGVC
                     ILGIKSDAVSIGKLERFVADWSKENDINLSDTEPKKNQKVAVIGSGPAGLACAGDLAK
                     KGYDVTIFEAMHEPGGVLTYGIPEFRLPKQAVVQPEIDNIRKLGVKIETNVIVGKTIT
                     VDELIEDEGFEAIFIGSGAGLPMFMNIPGENANGVFSANEFLTRVNLMKAYRDDYDTP
                     ISSGKKVAVVGGGNVAMDAARTALRLGSESYIVYRRSEKELPARAEEVHHAKEEGIIF
                     NTLTNPKEILVDENGYVKGMVCIRMELGEPDDSGRRRPIEIEGSEFVLDVDTVIMSLG
                     TSPNPLISSTTKSLDINKKRCLITDENGQTSKEGVFAGGDAVTGAATVISAMGAGKTA
                     AASIDEYLKAKVNA"
     gene            3072..>4056
                     /locus_tag="KPD58_003345"
     CDS             3072..>4056
                     /locus_tag="KPD58_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HBG0267072.1"
                     /translation="MKKREKVFDGLVFKHILILLILLVLIDLTSTIIYASNSMEITSR
                     NMIEASKRELENYLEVNISLLKALSQDDRFSNGETSLIEKGKLLSPYQKEYNLFMIGI
                     TDTKGNASSTYRENVNSIKDKPSFEKAIKTKQVVVSDIEVSNVTGDKVFIIYVPIVKN
                     NEMIGTIFASFYFQDVNNLISRSNFDDSIKFLMIDKDYTIISHPNKKYVNDKSKMLDL
                     EGNIIGTTKSEILKNINKKCQGDFLSWDNWRLYNVKYTNIKWTNWTLVSKCNIFKNFK
                     NLIVNFMIKLYFYIVIFMILWKLSNAKLIEQLKKLAYYDSLSGIKNKEKFRK"
BASE COUNT         1607 a          399 c          774 g         1276 t
ORIGIN      
        1 ggtattgcaa tagattaaag ccttatacac gtttagtttt taaagaaatt agtgttatta
       61 tgaaaaccta agttaattta ttaaataaat aattgaataa aaaaatttta ttatgtattt
      121 ataaaatttt tgttacaaat ttggcataat atagtatact ttaagtgtgt attaggttaa
      181 atctataaca aattagacgt atgatataga cttagtgttt attggcattt acaagaaatt
      241 tgttaatatc aactatttgt agtttgggca gttataatta ttataggggg gatgtccaat
      301 acaaaacaat caaccaagaa ctaatattat attataaaga gttatagttt tgtgtgttta
      361 tgtttttaat taaatttaaa gtgggaaaaa tatatttgtt agaatggaga atagcgagat
      421 gagtaataaa atagttagta agagacaatt aacagatagt atttatttaa tggaaataga
      481 ggcacctaga gttgcaaaat cttctcaacc aggtcaattt ataataatta aaaatgatga
      541 aaaaggagag aggattcctc ttacaatagc tgactatgat agagaaaaag gaacagtaac
      601 tattgtattc caaacagttg gtgcttcaac taaaaagtta gcaatgtttg aagaaaacga
      661 ttttgtaatg gattttgtag ggccattagg acaagcaagt gaatttatac atgaagatat
      721 agaagaatta agaaataaaa agatattatt tgtagctggt ggagttggtt ctgctccagt
      781 ttatccacaa gttaaatggt ttaaagagca tggattagat gtagatgtaa taattggtgc
      841 aagaactaaa gaattaatta tattagaaga tgatatgaaa aaagtagcta aaaatgtata
      901 tgtatcaaca gatgatggta catatggatt taatggaaga gttacagacc tattaaaaga
      961 tttagttgat aatcaaggta aaaaatacga ccaagcaata gtaattggac caatgataat
     1021 gatgaaattt atgtgccaat taactaaaga actaaatata cctactatag taagtttaaa
     1081 tacaataatg atagatggaa caggtatgtg tggtggttgt agagttagtg ttggaaatga
     1141 aactaagttt gcatgtgtag atggtcctga atttgatggt catttagttg attttgatca
     1201 agcaatgaga agacagtcta tgtacaagac tcaagaggga agagctatgc taaaacttga
     1261 agaaggcgat agtcatcatc atagtaattg tggatgtgga ggtaacaaat aatggatgct
     1321 aaaaaagtaa aagtaccagt tagagaacaa gaacctgcag tgagagcaac aaattttgat
     1381 gaggtatgct tagggtataa taaagaagaa gctatggcag aagctaatag atgtttagct
     1441 tgtaaaaaac caaaatgtgt gggtggatgt ccagtaggaa tcgacatacc agggtttata
     1501 acaaaaatta aagaagatga tatagaaggt gctgctaaag taatagctaa gagcagttca
     1561 ttaccagctg tttgtggtag agtatgccca caagaaagtc aatgtgaagg tgtatgtata
     1621 cttggaataa aatcagatgc tgtatcaata ggtaaattgg aaagatttgt agcagattgg
     1681 tcaaaagaaa atgacataaa tttatctgat acagagccta agaaaaatca aaaagtagca
     1741 gtaataggaa gtggtccagc aggtctagct tgtgctggtg atttagctaa aaaaggctat
     1801 gatgtaacaa tctttgaagc aatgcatgag ccaggtggag ttttaactta cggaatacct
     1861 gagtttagac ttccaaaaca agctgttgtt caacctgaaa tagacaatat aagaaaatta
     1921 ggtgtaaaaa tagaaactaa tgtaattgtt ggtaaaacaa ttacagtaga tgaacttata
     1981 gaagatgaag gatttgaagc tatattcata ggttcaggag ctggtcttcc aatgtttatg
     2041 aatataccag gggaaaatgc aaatggagta ttttctgcaa atgagttttt aactagagta
     2101 aacttaatga aggcttatag agatgattat gatacaccta ttagttctgg taaaaaagtt
     2161 gctgtagtag gtggtggaaa tgttgctatg gacgctgcta gaactgcatt aagattaggt
     2221 tcagaatcat atatagttta tagaagaagt gaaaaagagc ttccagcaag agctgaagaa
     2281 gttcatcatg caaaagaaga aggcattata tttaatacat taacaaatcc taaggaaatt
     2341 ttagtagatg aaaatggata tgttaaaggt atggtttgta taagaatgga attaggagag
     2401 ccagatgatt ctggaagaag aagacctata gaaatagaag gttctgaatt tgtacttgat
     2461 gtagacacag ttataatgtc acttggaaca agcccaaatc cactaatatc ttctacaaca
     2521 aagagtcttg atataaataa gaagagatgc ttaataacag atgaaaatgg tcagacttct
     2581 aaagagggtg tatttgcagg tggagatgct gttactggag cagcaacggt aatatctgca
     2641 atgggtgctg gtaaaacagc agcagcatca atagatgaat atttaaaagc aaaagttaat
     2701 gcttaatttg taaattaaag agagggtgtc tcaaaatgaa ctttttagtt catgagacac
     2761 tctttttgta caaaatcaaa tatgttttaa tcatttcaac aatgaaaatt tcattcctgt
     2821 agaaagtcta acttttaggt cttagtacaa tctctatttt gatgtagcca cttttttaga
     2881 tggtttaata atttatcagt tattattgta atatttgata ctaagtaaca taactttgtc
     2941 taactatgat aaaatagtta tacaataaag gtcttgtaag gagtgaataa tgtgtggcta
     3001 ataaaatcag atttgtcaaa tctaacatta aataatagaa aaataaattg tgtaattaat
     3061 ggtattttaa tatgaaaaaa agagaaaaag tatttgatgg gctagttttt aagcatatat
     3121 taatcttatt aatactatta gttttgattg atttaacaag taccatcata tatgcaagta
     3181 actctatgga aattacttct agaaatatga ttgaggcatc aaaaagagaa cttgaaaact
     3241 atttagaagt aaacatatct ttattaaaag ctcttagtca agatgataga ttttctaatg
     3301 gtgaaacatc actaattgaa aagggaaaat tattaagtcc atatcaaaag gaatataatc
     3361 tttttatgat tggtataaca gatacaaaag gaaatgcttc aagtacatat agggaaaatg
     3421 ttaactcaat aaaagataag cctagttttg agaaagcaat taaaacaaag caagtagtag
     3481 tttcagatat tgaagtcagt aatgtaacag gtgacaaagt atttatcata tatgtaccaa
     3541 ttgtaaaaaa taatgagatg attgggacca tttttgcttc tttttatttt caagatgtaa
     3601 ataatttaat aagtagaagc aattttgatg attctattaa atttctaatg atagataaag
     3661 attatactat tatttctcat ccaaacaaaa aatatgtaaa tgataaaagt aagatgcttg
     3721 atttagaagg gaatattata ggaaccacta aaagtgaaat cttaaaaaat ataaataaaa
     3781 aatgtcaagg tgatttttta tcttgggata attggagatt atataatgta aaatatacga
     3841 acataaagtg gactaattgg actttagtat caaaatgtaa tatatttaag aactttaaaa
     3901 atctaatagt taattttatg ataaagttgt atttttatat agtaatattt atgatattgt
     3961 ggaaattaag taatgctaaa ttaatagaac aacttaaaaa attggcttat tatgattctt
     4021 taagtggaat aaaaaataaa gaaaagttta gaaaag
//
LOCUS       DAELLV010000278         4010 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.278, whole genome shotgun sequence.
ACCESSION   DAELLV010000278 DAELLV010000000
VERSION     DAELLV010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.278"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(46..1143)
                     /gene="ychF"
                     /locus_tag="KPD58_003346"
     CDS             complement(46..1143)
                     /gene="ychF"
                     /locus_tag="KPD58_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBG0267073.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITQAGAESANYPFCTIDPNVGVVSV
                     PDERLNKLQELYNSEKIVPTAIEFCDIAGLVRGASKGEGLGNKFLSHIREVDAIVHVV
                     RCFEDENVVHVDGSVDPLRDIETINLELIFSDIEILERRINKTQKAAKADKTLGSELD
                     LLKSIMSTLEESKCVRTMEFTEDEQTFVNSLDLLTSKPVIYASNVSEDDLADNGENNK
                     YVQQVKAFAETEDAEVVVVCAQIEAEISELDSAEEKKEFLETLGLEQSGLDKLIKSSY
                     ALLGLISFLTAGPKEVRAWTIKVGSKAPQAGGKIHSDIERGFIRAETIAFNDLVEHGT
                     MAAAKEKGLVKLEGKEYIVKDGDVILFRFNV"
     gene            1459..2724
                     /locus_tag="KPD58_003347"
     CDS             1459..2724
                     /locus_tag="KPD58_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBG0267074.1"
                     /translation="MVNTKKIINLGNNINLTLIKTEKFKSNLISLYVQRLLDEKETTK
                     NALIPSIIASGSAKYPSARAISNKLDDLYGSSMGADAVKRGERQVLSFKVINISEKYL
                     DESIFEEVVEFFNEVINNTLVVDGGFKEEYLNIEKENLREKIQSIINDKKEYAQDKCI
                     EAMCKGERYSVSEFGYEDEIDSITSRELYEHYKNILKTSPIDIVVEGNFDEDKVVDII
                     SKNLKFEREEIINIPRADFIKNVDEVKVIDEQMEITQGKLVMGYRANVDYADIDKYYA
                     LVVGSNVLGGGPHSKLFVNVREKESLCYYIFSSIEKYKSIMFISSGIETKDYDKAVEL
                     IKKQVESVKAGDISDEELENSKLALVNSMKSITDNIGGMSDFVFSQSMAKTNSEVQDI
                     ISSIEKVTKKDIVEAIKNIELDTVYFLRN"
     gene            2739..>4010
                     /locus_tag="KPD58_003348"
     CDS             2739..>4010
                     /locus_tag="KPD58_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBG0267075.1"
                     /translation="MEKIVNDILKEEVYYEKLQNGLDVYFMPKRGFMKKYAILATNYG
                     SNDLEFVPIGEDKKIRVNEGIAHFLEHKMFEQPDGGDAFDKFSKLGVNANAFTNFTMT
                     AYLFSATENFYESLEHLIDYVQTPYFTDENVEKEKGIIAQEIKMYNDDPDWNVYFNCL
                     KAMYVNYPARIDIAGTVDSIYKITKEELYKCYNTFYNPGNMALFVVGDLDVEKVIDVT
                     KKSNNYKVDRLSKSIERFYPEEPESVKEKEVIEKFPISMPMFNIGFKDSNVGLKGKEL
                     LRKEIVTDILVGMLFKKGSKLYEDLYMQGLINENFGAGFSSQVDYAFSIIAGDSKEPK
                     KVKEIILDYIEKSKKEGLSKEEFERTKKKKIGSFIKCFDSINFIGNSFISYVFKDINL
                     LDYLDVIKDITFEEVEERLKEHFKEEYCVISI"
BASE COUNT         1535 a          456 c          644 g         1375 t
ORIGIN      
        1 tttagttatt atatataata gtcaaagtgt atacgattaa tcattttaaa cattaaatct
       61 aaataatatt acatctccat ctttaactat gtattcttta ccttctaatt taaccaaccc
      121 tttttcttta gcagcagcca tagtaccatg ctcaaccaaa tcattaaagg ctattgtttc
      181 tgctctgata aatcctcttt ctatatcaga atgtattttt cctcctgcct gaggagcttt
      241 acttccaact tttatagtcc atgctctaac ttcttttgga ccagcagtta agaatgaaat
      301 taaaccaagt aaagcataag aagattttat aagcttatca agccctgatt gttcaagacc
      361 taatgtttct aaaaactctt tcttttcttc agcagaatca agttcagaaa tttctgcttc
      421 tatttgtgca catactacaa caacttcggc atcttctgtt tctgcaaatg ctttaacttg
      481 ttgaacatat ttattatttt ctccattatc agctaaatca tcttctgata catttgaagc
      541 atatattaca ggcttagatg taagtaaatc caatgaattt acgaatgttt gttcatcttc
      601 tgtaaattcc atagttctta cacacttact ttcttctaaa gtagacatta ttgattttaa
      661 taaatcaagt tcagaaccta aagttttatc agccttagca gctttttgag ttttattaat
      721 tcttctttca agaatctcta tatctgagaa aattaattct aagtttatag tttctatatc
      781 acgaagtggg tctacagaac catctacatg tacaacattt tcatcctcaa aacatctaac
      841 aacatgaact attgcatcaa cttctcttat atgtgataag aatttatttc ctaaaccttc
      901 tcctttagaa gctcctctaa ctaaacctgc tatatcacaa aattctatag cagtaggaac
      961 tattttttca gaattatata attcttgtag tttgtttaat ctttcatctg gaacagatac
     1021 aactcccaca tttgggtcta ttgtacaaaa agggtaattt gcagattctg cacctgcttg
     1081 agttattgcg ttaaatagtg tgcttttacc tacatttggt aaaccaacta tacctaattt
     1141 catattaatc gtcctctctc ttattctttt ttatattctt tatataactt taaacaatca
     1201 atctatagga ttgcaaaatt gttttaatta tagacataca tgttgattat acatcatata
     1261 tatcaagctt gtaaactaga ctttaaagca tattgattta aataatattt gtatttaatt
     1321 taaataaaaa tattgctttt agaaaataat aaaaaggata taattaaata gaaggttttt
     1381 atatgtgagt atatgtaatt aaaaaataaa taaatttcaa ttttaaaatt gatgtttaaa
     1441 taaaaatagg aggtacatat ggttaatact aaaaaaataa taaatttagg taataatatt
     1501 aatcttactt tgataaaaac agaaaagttt aaatcgaatc ttataagttt atatgttcag
     1561 agactattgg atgaaaaaga aactacaaaa aatgctctaa taccatcaat aatagcaagt
     1621 ggaagcgcta aatatcctag tgctagagca atatccaata aattagatga tttatatggt
     1681 tcatctatgg gagctgatgc tgtaaaacgt ggagaacgtc aagtattgag ttttaaagta
     1741 attaacataa gtgaaaagta tttagatgag agtatatttg aagaagtagt agaatttttt
     1801 aatgaagtaa taaataatac gcttgttgta gatggtgggt ttaaagaaga atatttaaat
     1861 attgaaaaag agaatttaag agaaaagata caatcaataa taaatgataa aaaggaatat
     1921 gcgcaagata aatgtattga ggctatgtgt aaaggagaga gatatagtgt aagtgaattt
     1981 ggttatgaag atgaaataga tagtataact tcaagagagt tatatgaaca ttataaaaac
     2041 atacttaaaa catcacctat agatatagtt gtagaaggta attttgatga agataaagtt
     2101 gtagatataa taagtaaaaa tctaaaattt gaaagagaag aaattataaa tataccaaga
     2161 gcagatttta taaaaaatgt ggatgaggta aaagttatag atgaacaaat ggaaataact
     2221 caaggtaagt tagttatggg ttatagagca aatgtggatt atgcagacat agataaatat
     2281 tatgctttag tagttgggag caatgtctta ggtggtggtc cacactctaa attatttgtt
     2341 aatgtaagag aaaaagaaag tttatgttac tatatattct cttcaataga gaagtataaa
     2401 agtatcatgt ttatatcatc tggtatagaa actaaagatt atgataaagc tgtagaattg
     2461 ataaaaaaac aagtagaaag tgtaaaagca ggtgatatat ctgatgaaga attagaaaac
     2521 agtaaacttg ctttagttaa ttctatgaaa tctataactg ataatatagg tggtatgtct
     2581 gattttgttt tctctcagtc tatggcaaag acaaattctg aagtacaaga cataatatct
     2641 agtatagaaa aggttacaaa aaaagatata gttgaagcta taaaaaatat agagcttgat
     2701 actgtatact tcttgagaaa ttaatggagg agatgtctat ggaaaaaata gttaatgata
     2761 tattgaaaga agaagtttat tatgagaaat tgcaaaatgg attagatgta tattttatgc
     2821 ctaaaagggg ttttatgaaa aagtatgcta ttttggctac taattatggt tcaaatgact
     2881 tagaatttgt accaattgga gaagataaga aaattagagt gaatgaaggt attgcacatt
     2941 ttctagagca taagatgttt gagcaacctg atggaggaga tgcatttgat aaattttcaa
     3001 aattaggagt aaatgcaaat gcatttacta attttacaat gacagcctac ttattttctg
     3061 ctactgaaaa tttttatgag agtttagaac atctaattga ctatgttcaa acaccttatt
     3121 ttacagatga aaatgttgaa aaggaaaaag gtataatagc acaagagata aaaatgtaca
     3181 atgatgaccc tgattggaat gtatacttta attgcttgaa ggcaatgtat gtaaattatc
     3241 ctgctagaat tgatatagca ggtactgttg atagtatata taagataact aaggaagagc
     3301 tatataaatg ttataatact ttctataatc caggaaatat ggctttattt gttgttggtg
     3361 acttagatgt agaaaaagtt atagatgtaa ctaaaaagtc aaacaattac aaagtagaca
     3421 gactctctaa gagtatagaa agattttatc cagaagaacc agaaagtgta aaagaaaaag
     3481 aggttataga aaaattccct atatctatgc ctatgttcaa cataggtttt aaagatagca
     3541 atgttggttt aaaagggaaa gaacttttac gtaaagagat tgtaactgat atacttgtag
     3601 ggatgttatt taaaaaagga agtaagcttt atgaagattt gtatatgcaa ggtttaataa
     3661 atgaaaattt tggagctgga ttttcgtctc aagtggatta tgcttttagc ataatagctg
     3721 gagattctaa agagcctaaa aaagtaaaag aaataattct tgattatata gaaaaatcaa
     3781 aaaaagaagg attatcaaaa gaagagtttg agagaactaa aaagaaaaaa atagggtcat
     3841 ttattaagtg ctttgattct ataaacttta taggtaatag ttttatatct tatgttttta
     3901 aggatataaa tctattggat tatttagatg ttattaaaga tattactttt gaggaagttg
     3961 aagaaagatt aaaagagcat tttaaagaag agtattgtgt tatttctatt
//
LOCUS       DAELLV010000279         3999 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.279, whole genome shotgun sequence.
ACCESSION   DAELLV010000279 DAELLV010000000
VERSION     DAELLV010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.279"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(352..1221)
                     /locus_tag="KPD58_003349"
     CDS             complement(352..1221)
                     /locus_tag="KPD58_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBG0267076.1"
                     /translation="MVIIKAIELYKKLDLEFNIKNINDDWSFMNFENDFITPEFRKKY
                     IGLVLDNAQNINKVYTTTFPDKEIIKQVIDKDEKDILIFSHHAMGYIASDEGFPFHDI
                     PLSYMEEMKNRRISFYVLHSPLDNYSDYSTSVSFAKALGLEIVKPFCKYDDKIEVGVI
                     CKTSLKSIEEIKELIKKSVGHDVKLYDYGDSVLKDGLVAIAAGGGSYPFVAREVAELG
                     INLYITGFTKPLKHFEPVLEFHQIAKDNLINVIGATHYSTEKFACMGMVDYFKNLGLE
                     SEFLQGKYYLEDL"
     gene            complement(1525..1995)
                     /locus_tag="KPD58_003350"
     CDS             complement(1525..1995)
                     /locus_tag="KPD58_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HBG0267077.1"
                     /translation="MKYQVSLLAVKDIEVSKKFYKELFEQEIELDLGKNVTFKGGFAI
                     QEDFAWLTGINPDTIIQKSNNMELYFEVDDFDEFIEKLNYYKDVEFVHKPLKHEWQQR
                     VVRIYDPDKHIIEIGESMEVIARRYLSEGYSVEETSKIIQHPIKFVKQCENSKE"
     gene            complement(2593..2982)
                     /locus_tag="KPD58_003351"
     CDS             complement(2593..2982)
                     /locus_tag="KPD58_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0267078.1"
                     /translation="MTIKKLPQSELKIMKFIWKSDSKVTSRDIVLGMEQKYQWKQTTT
                     LTLLSRLVVKRFLNSQKIDKYTHYEVLIKEKEYIGVETRDFFRNIHDSSIKSLLLSLH
                     ENINLSKDDILLIEEWIKNLKEEEKDV"
BASE COUNT         1357 a          667 c          272 g         1703 t
ORIGIN      
        1 ttaatttagt gcatatttga cactttacct tttattatac catttattat cataaatata
       61 ataattctat ttttttaaaa taaatattta taataatcta aaataaatat ttataaattc
      121 taatcaatcg taaataataa tctaaaatac atcaaaaaat aaactaaaaa atatcgaaaa
      181 ataatttaaa agacatcaaa actaaatctt ctaatcagca attaaaagtc tattttctta
      241 gtttaaaaaa taaagataga ttttttaaaa acactggata aataggtaaa aaaataaaca
      301 gttagtaaaa taaattttta ctaactgttt atttttattg tttaatatgt cctataaatc
      361 ctctaagtaa tactttcctt gcaaaaattc tgattctaaa cctagatttt taaaataatc
      421 aaccatgccc atacacgcaa atttttctgt agaataatga gtagcaccta taacattaat
      481 taaattatct tttgctattt gatggaactc taatactggt tcaaaatgtt ttaaaggctt
      541 agtaaaccca gtaatatata gatttatacc aagttcagca acttctcttg ctacaaatgg
      601 ataacttcct ccccctgctg caatagcaac taaaccatct tttaatacag aatctccata
      661 atcatacaat ttcacatcat gaccaacaga ttttttaatt aattccttaa tctcttctat
      721 tgattttagt gatgttttac atataactcc tacctcaatt ttatcgtcat atttacaaaa
      781 tggtttgact atttctaaac ccagagcttt tgcaaaacta acagaagttg aatagtcact
      841 atagttgtct agtggagaat gtaatacata aaaagaaatt ctacgatttt tcatctcttc
      901 catataagat agtggtatgt cgtgaaatgg aaaaccttca tctgatgcta tgtatcccat
      961 tgcatgatgt gaaaaaatta aaatatcttt ttcatcttta tctataactt gcttaattat
     1021 ttctttatca ggaaaagtag ttgtgtatac tttattaata ttttgagcat tatctaatac
     1081 taatcctatg tatttttttc taaattctgg agtaataaaa tcattttcaa aattcataaa
     1141 actccaatca tcattaatat ttttaatatt aaattctaaa tctaattttt tataaagttc
     1201 aattgcctta ataataacca ctcctttata ataatattga attactacat attaatatag
     1261 aaatttccga taataaactt tatacgttta agtattccta cttcaaatta aaaatattat
     1321 aaatattcaa atttattaat atattttaat attatattta ttttttcttt attaatattc
     1381 cccttatttt tatttatttt ttctttatta atattttctt tattttaatt taatttatga
     1441 catttgtcaa atactagtcc tatatattat cttgaattag ttctattaca attaatttct
     1501 actaaataat tataatttaa acaatcattc cttactattt tcacactgct ttacaaattt
     1561 aattggatgt tgaattattt tagaagtttc ttctacagag tatccctcag ataaatatct
     1621 tctagctata acttccatag actctcctat ctcaataata tgcttatccg ggtcataaat
     1681 acgcactact ctttgttgcc attcatgttt tagtggctta tggacaaact ctacatcttt
     1741 ataataattg agtttttcaa taaattcgtc aaaatcatca acctcaaaat aaagttccat
     1801 attatttgat ttttgtataa ttgtatctgg attaatccct gtaagccatg cgaagtcttc
     1861 ttgtatagca aatcctcctt taaatgttac atttttacct aagtccaatt caatttcttg
     1921 ctcaaataat tctttgtaaa atttttttga tacttcaata tccttcactg ctaataatga
     1981 aacctgatat ttcataaatt ttcctcctaa ttttttattt ctattttttc agtatcttct
     2041 attaacattt ttttctctat ctcttcagta ccttttatta atcctttatt tttacttttt
     2101 tagtatcttc tattaacact tttgtttcta cctcttcagt atcttatatc aatcttttat
     2161 tttttcaata tcttctgtta acacttttat ctctatctct ttaatatctt atattaatcc
     2221 tttattttta ttttttcaat atcatcatta attcctattt attttatcat cttaaatacg
     2281 aaaacactgt gtcatatttg caaaatatat attatcatct atattaaagc ttcacaattt
     2341 atatttaaaa attctcttta attacactac ctctatttaa aaagctacct taattatact
     2401 tataagtaaa ggtagctttt taaatttaat taaatagaat atccactatt aatttatatt
     2461 taatttattt agattcacat tatagtatca taatccactt ataatcattt gtcatattac
     2521 ttaagtagac taatatatta cataaataca cttacataca ctttatattg atgctataag
     2581 aaatacgttt gcttatacgt ctttctcctc ttcttttaga ttttttatcc actcttcaat
     2641 aagtaaaata tcatctttgc tcaaattaat attttcatgt aatgacaaca ataaactttt
     2701 tatagaagaa tcatgaatat ttctaaaaaa atctcttgtc tcaactccta tgtattcctt
     2761 ttctttaatt aaaacttcat aatgcgtata tttatctatc ttttgagaat ttagaaatct
     2821 ttttactact aatctagata aaagagtcaa agttgtcgtt tgtttccatt gatatttttg
     2881 ctccattcct aaaacaatat ctctagatgt tactttggaa tctgatttcc atataaattt
     2941 cattatcttt aattctgatt gtggcaactt ttttatagtc atgataaaac cttgctactc
     3001 agaatataga caagcaatat ccataataaa atttttcgat taaatacatc ttatacggat
     3061 ttatataagc tcagacttat aagatatttt tcaatcttaa tcctaaattt agctatttgt
     3121 acacggtaaa tatatataaa taatgtcaaa attctcttgt attatattgc ttaattttat
     3181 gaaatatttt gtactcagct atctattttg gatacttgtc cagtagctct cctttctttt
     3241 aaactctttg attttaaata tttgtatttc agtaaatcct acttcctaaa ttttttattt
     3301 actacaactt attactgaac ttcctacttc ctaaactttt tatttactac aacttattac
     3361 tgaacttctc acttactaaa tttcttactt actataaatt attactaaac ttcctacttt
     3421 ctaaatttct tacttactat aaattattac taaacttcct actttctaaa tttcttactt
     3481 actataaatt attactacta aacttcccac ttactaaatt tcttacttac tataaattat
     3541 tactaaactt cctactttct aaatttctta cttaccacaa attattacta aacttcctac
     3601 tccctaaatt tcttacttgc tataaattat tactaaactt cctactttct aaatttctta
     3661 cttgctacaa attattacta aatttcttac ttgctataaa ttattactaa taaacttccc
     3721 acttcctaag tttcttactt actactactt ataactaaac ttcccacttc ctaaatttct
     3781 tacttactac cacttataac taaacttccc acttcctaaa tttcttactt actaccactt
     3841 atcactaaac ttcccacttc ctaaatttct tacttactac cacttatcac taaacttcct
     3901 actttctaaa tttcttgctt cttaagaata atgttaattt taattttaag attatagttt
     3961 ttaccttgtt taattgaaac attctatata attatatcc
//
LOCUS       DAELLV010000280         3972 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.280, whole genome shotgun sequence.
ACCESSION   DAELLV010000280 DAELLV010000000
VERSION     DAELLV010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3972
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.280"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            159..1112
                     /locus_tag="KPD58_003352"
     CDS             159..1112
                     /locus_tag="KPD58_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBG0267079.1"
                     /translation="MKKRYLVIGIIILSYFSLFIGAEDINIMHIFAKNQHKLMIFIMS
                     RIPRLISILIAGVGMSIAGLIMQQISKNKFVSPTTGATIDAAQFGIVICMLLVPTASI
                     FTKTIIAFVFSLVGTFTFMKIIGKLQFKNIIFVPLVGIMFGNIIGSMTDFIAYKYDLS
                     QNVSSWMQGDFSMILKGNYEILYITIPLIILAYIYANKFTVVGMGMDFATNLGLSYKR
                     IVNIGLIIVALVTVCVVVTAGNIPFIGLIVPNIVSLYMGDNIRASIWYTGLLGAIFVL
                     ICDIFGRIIIYPYEISIGLTVGVIGSILFLYLILRRNVNEA"
     gene            1102..2058
                     /locus_tag="KPD58_003353"
     CDS             1102..2058
                     /locus_tag="KPD58_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBG0267080.1"
                     /translation="MKLSLNQKIYFVSILLFISMALFIIYGIDMNHLEYALSQRIPKI
                     FAMVLGGGCIAFTTVVFQTITNNQILTPSVLGLDSLYVMIQTIIVFIFGSSSSLIINE
                     NYNFIINVAMMIGASLLLYKTLFEKNKNNIFFLILVGMIFGTLFKSATTFIQVMIDPN
                     EFLALQTSIMASLNNINTNVLLIAFIIIIAIIPFIYDEIKYLDVLSLGKEQAINLGVD
                     FDKAVKKMIILIAILVSISTALIGPMTFLGLLLANITREIFKTYKHTYLISGSMLIGM
                     ITLIIGQFFIQHVFKFDTTLSVVINFIGGIYFIQLLLKGANR"
     gene            2055..2810
                     /locus_tag="KPD58_003354"
     CDS             2055..2810
                     /locus_tag="KPD58_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0267081.1"
                     /translation="MIEIKNIFKRYKNKNVVDDVSFSIEKGKITSFIGPNGAGKSTVL
                     SIVTRLIGGDGGEVIIEGKSLTNYSNKELAKKIAILKQSNNITLKLTIRELVGFGRFP
                     YSEGNLTKEDENYIDEAIEYMKLTDIQHKYLDELSGGQRQRAYIAMVIAQDTEYILLD
                     EPLNNLDMNHSVQMMKVLRGLCDELDKTIVLVMHDINFASCYSDNIVALKNGKVEKVG
                     RTDEIVNEKVLEDIYEMNFNIKNINGNRICIYF"
     gene            2902..3867
                     /locus_tag="KPD58_003355"
     CDS             2902..3867
                     /locus_tag="KPD58_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG0267082.1"
                     /translation="MNKKAAIVAAVAIIGLVTVFALGGSKKNESKTSENSNNTMKITH
                     NLGETDVKLNPKKVVVFDYSALDTMDALGVAETLVGLPKASLPASLEKYKDDKYADLG
                     GLKEPDLEGIKSANPDLIIINGRQEDFYEQLSKIAPTISTSKDDKKYLESVKNNIDKI
                     AKIFGVEEKANQEFSKIEKKIETLNKKVTDKNLNALTIMVNEGNLSVFGEESRFSILY
                     NSFGFENKDKNIKESSHGQNITFEYIAKQNPEVMFVIDRGIATGSDVKESSTAKSVLN
                     NDIIKSMDAYKNDNIIYLDSPTWYVNDGGLTSLNKMIDDASKAVN"
BASE COUNT         1596 a          376 c          672 g         1328 t
ORIGIN      
        1 attttaatgt tactagaagt ttcaattcta gtaacatttt ttatgttaaa aacagatata
       61 gatatgtatt gacaatgata attattattg ataaactata aaagtgaaaa tgataataat
      121 tttcatttta attaataaaa gatttttaag gagggagaat gaagaaaagg tatttagtca
      181 taggaataat aattttgtct tatttttctt tatttatagg agctgaagat ataaatataa
      241 tgcatatatt tgccaaaaat cagcataaac ttatgatatt tatcatgagt agaataccaa
      301 gattaataag tatattgatt gctggtgtag gaatgagcat agcaggtctt ataatgcaac
      361 agataagtaa gaataaattt gtatcaccta caacaggtgc tacaatagat gcagcacaat
      421 ttgggatagt aatatgtatg cttttagtac caactgcctc tatttttaca aagacaataa
      481 tagcatttgt attttctctt gtagggactt ttacgtttat gaaaataata gggaagttac
      541 aatttaaaaa tataatcttt gtaccattag taggtataat gtttggaaat ataatagggt
      601 ctatgacgga ttttatcgcg tataaatacg atttgagtca aaatgttagt tcttggatgc
      661 aaggtgactt ttctatgata cttaaaggaa attatgagat attatatatc acaatacccc
      721 ttataatatt agcatatatt tatgcaaata aatttactgt tgttggtatg ggtatggatt
      781 ttgcgacaaa cttaggtctt agttacaaaa gaattgtcaa tataggtctg ataatagtag
      841 cacttgttac agtatgtgta gtcgttacag ctggtaatat cccttttata ggtctaatag
      901 ttccaaatat cgtatccttg tacatgggtg ataatataag agcaagcata tggtatactg
      961 gtttacttgg agcgatattt gtacttatat gtgatatttt tggaagaatt attatttatc
     1021 catatgaaat atctataggg cttacagttg gagttatagg aagtatacta ttcctttatt
     1081 taatattaag gaggaatgtg aatgaagctt agtctcaatc aaaaaattta ctttgtaagt
     1141 atattactat tcatatcaat ggcacttttt ataatttatg gaatagacat gaatcattta
     1201 gaatatgcat tatcacaaag aataccaaaa atatttgcta tggtattagg tggaggatgt
     1261 atagcattta ctactgtagt gtttcaaaca attacaaaca atcaaatatt aacaccaagt
     1321 gtactaggtt tagattcatt gtatgtaatg attcaaacta taatagtatt catatttggt
     1381 tcaagcagta gtctgattat aaatgaaaat tataatttta ttattaatgt agctatgatg
     1441 attggagctt cattgttatt atataagacg ttatttgaaa aaaataaaaa taatattttc
     1501 tttttaatcc tagtaggaat gatatttgga accttattta aaagtgcaac aacatttatt
     1561 caggtaatga tagaccctaa tgaattttta gctttacaaa ctagtataat ggcaagttta
     1621 aacaatataa acactaatgt attgttaata gcatttataa taattattgc cataatacct
     1681 tttatatatg atgaaataaa gtatttagat gtattgtcgt taggtaaaga acaagcaatt
     1741 aatttaggtg tagattttga taaagcagtt aaaaaaatga taatattaat agcaatttta
     1801 gtctcaatat caacagcact tattggacct atgacatttt taggactttt acttgctaat
     1861 ataacaagag aaatttttaa aacatataag catacatact tgatttcggg ttctatgttg
     1921 atagggatga ttacattgat aataggtcaa ttttttatac aacatgtatt taagtttgat
     1981 acaactttaa gtgtagtaat aaattttata ggaggaattt attttattca attactatta
     2041 aagggggcaa atagatgata gagataaaaa atatatttaa aagatataag aataaaaatg
     2101 ttgtagatga tgtctctttt agtatagaaa agggaaagat aacatctttt ataggaccaa
     2161 atggagctgg aaaaagtaca gtactatcaa tagttacaag acttatagga ggtgatggtg
     2221 gagaagtaat aatagaagga aaaagcttga ctaattatag caataaagaa ttagcaaaaa
     2281 aaatagctat tcttaaacaa tctaacaata taactttaaa gcttacaata agagaattag
     2341 ttggatttgg aagatttcca tatagtgaag gtaatctaac taaagaggat gaaaattata
     2401 ttgatgaagc aatagaatat atgaagttga ctgatataca acataagtat ttagatgaat
     2461 tgagtggagg tcaaagacaa agagcttata tagcaatggt aatagctcaa gatacagagt
     2521 acatattact tgatgaacca ctgaacaatt tggatatgaa ccattccgta cagatgatga
     2581 aggtacttag aggtctttgt gatgaattag ataagactat agtattagta atgcatgata
     2641 taaattttgc atcatgttat tcagataata tagttgcatt aaaaaatgga aaagttgaaa
     2701 aagtaggtcg tacagatgag atagtaaatg aaaaagtttt agaagatatt tatgagatga
     2761 attttaacat taagaatata aatggaaatc gaatctgtat ttatttttaa aatattgtat
     2821 atgaaaataa acttgctata tgatttttta taaagttttt aaattaaaat aaagattaat
     2881 aggagagagg aaaatattga tatgaataaa aaagcagcaa tagtagcagc agtagctatt
     2941 ataggattag tgacagtgtt tgcattaggt ggaagtaaaa agaatgaatc aaaaacatca
     3001 gaaaattcaa ataatactat gaagataact cataacttgg gagaaacaga tgtaaaatta
     3061 aatcctaaaa aagtagtggt atttgattat tcggctttag atacaatgga tgcattaggt
     3121 gtagctgaga ctttagtagg acttccaaaa gcgagtttac cagcatcctt agaaaaatat
     3181 aaggatgaca agtatgcaga tttaggagga ttaaaagaac cagatttaga aggtataaag
     3241 tcggcaaatc cagatttaat tataataaat ggaagacaag aagattttta tgagcaattg
     3301 tcaaaaatag caccaacaat aagtacaagt aaagatgata agaagtactt agaatctgtt
     3361 aaaaataata tagacaaaat agcaaaaata tttggagtag aagaaaaagc aaatcaagaa
     3421 tttagtaaaa ttgaaaagaa aatagaaact ttgaataaaa aagtaacaga taaaaattta
     3481 aatgctttaa ctataatggt aaatgaaggt aatttaagtg tatttggtga agaatcaaga
     3541 tttagtatat tatacaatag ttttggattt gaaaataaag ataaaaatat aaaagaatca
     3601 agtcatggac aaaatattac ttttgaatat atagctaaac aaaatccaga agttatgttc
     3661 gttatagata gaggaatagc tactggcagt gatgtaaaag aaagctcaac tgcaaaatct
     3721 gttttgaaca atgatataat aaaatctatg gatgcatata agaatgataa tataatatat
     3781 ttagattcac caacatggta tgtaaatgat ggtggtctaa catctttaaa taaaatgata
     3841 gatgatgctt caaaagcggt aaattaataa tccgatataa ccaaaatgta ttttttataa
     3901 aatgattatc aaataaacta ggagttttta agaactccta gtttattttt tgcataaaaa
     3961 agatatagtt ta
//
LOCUS       DAELLV010000281         3938 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.281, whole genome shotgun sequence.
ACCESSION   DAELLV010000281 DAELLV010000000
VERSION     DAELLV010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.281"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            189..1694
                     /locus_tag="KPD58_003356"
     CDS             189..1694
                     /locus_tag="KPD58_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBG0267083.1"
                     /translation="MKIQNTEWGYIEWKHTYDENNPKQAMNIYIAVTMPGKKHFNHVH
                     YGQEQMIYILEGEGLYIINGVWKPFYQGMIFYIESGSTHETINTGDREIKELIVSNNV
                     DDVGESEVIDINPNNYLKKTLINYSESTLNLYAAVESIRGQFIDPFKIPLIIYDDSWN
                     IVLKNPYFPLFCFEKCNPMKFPQNCDCMNQKSSNQFVCEYGITIYNIPILYKSNSIGV
                     IRGGYVLLSDLNLDTEHNNLYDIPEGAARSIKRLLKQISKNIINFCSFNDIRKDLQEK
                     EKTIARTYHYGEQLEMNLKVAQDMVTNLRINHHFLFNTLNSMASIALDDGSYDLYSAI
                     IDLSRMFRYTMRSDLRFVELESEILYIKNYLNLQKLRYGDALKVKYLIPEKLYNLSVP
                     FNFIQPIVENAFTHGFRDIDTEKRIEIIARLDSQYAIIEIHNNGTILDGNNIDKIKAG
                     IRSNNGHGLSLIYTKFTSAYGNNFDMDIKSSDNEGTYIMIKIPIENYKEYV"
     gene            1808..2506
                     /locus_tag="KPD58_003357"
     CDS             1808..2506
                     /locus_tag="KPD58_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBG0267084.1"
                     /translation="MRRALIIDDEEAVLKIITHFIEMKDMPIKIVGKATSGDEAVDKI
                     IGLEPDIVFIDIQMPIYNGLEVIEKTSHLSKKINFVVITAFNYFEYAQKALRLNVKDI
                     LLKPLDMKEFTKSVEKIIGYQYTNNDLLNEILEYINLNYSNDLKLNDCARLFLTNPSN
                     ISRIFKSNLNTTFISYLNHIRIEKSIELLENTTKSINEIAEIVGYNSLNNFYKNFKIE
                     KGMTPKVYKLNTKN"
     gene            2791..3879
                     /locus_tag="KPD58_003358"
     CDS             2791..3879
                     /locus_tag="KPD58_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0267085.1"
                     /translation="MSDKVNMKNIICIAGAYLATAIGSGFATGQEILQFFASQGYMGL
                     IGALISMVLFSFLGAEVITRGRELKLKEPTRIYTFYCGKILGKFYEWFSPIFLFGILI
                     VMISGAGSTLTEYYGINPYLGRVLMAVATLITVSMGLEKLVSILGRIGPVIAVFTIAV
                     GLISLFKHFDGLSMVGEAMKNIEVTKASESPIVSGVIYNTLNIIVMIVFLTGMGASIE
                     SKKDAFWGGLLGGIVFMTAGIVMYLAMISDIGNLYMKEIPSLYLADNISPIVGVCFSV
                     ALILGIYTTAVPLLWSVTNRIVEDEHPKFKLVTTVIAILACIGGFLPFGKLVNILYPY
                     TGYMGLLILVCMVYKAITCRKKSTQSQE"
BASE COUNT         1553 a          389 c          639 g         1357 t
ORIGIN      
        1 aaaaaaatag ttaatataaa agtgatacaa taataataga agtaataaaa aaaaataatt
       61 aatataaaaa tgtgtacaaa ctaatgaata ttgatatagt aagctatcat atgaaagagt
      121 atgtacacat tttttttact aatttttcac aatatgaaca taaataattt acaggaaagg
      181 aatcttttat gaaaattcaa aatactgagt ggggatatat tgaatggaaa catacttatg
      241 atgaaaataa tccaaaacaa gctatgaata tatacattgc agtaactatg cctggtaaga
      301 aacattttaa tcatgttcat tatggtcaag agcagatgat atatatttta gagggagagg
      361 gtttgtatat tataaatgga gtttggaagc cattttatca aggtatgatt ttttatatag
      421 aatctggtag cacacatgaa actataaata caggtgatag agaaataaaa gaacttattg
      481 tttcaaataa tgtagatgat gtaggagaga gtgaagttat agatattaat cctaataatt
      541 atttaaagaa gaccttgata aattatagtg aaagtacatt aaatttgtat gcagcagtag
      601 aatcaattcg tggtcaattt atagacccgt ttaaaattcc attaattata tatgatgatt
      661 catggaatat agttttaaaa aatccttatt ttccactttt ttgttttgaa aaatgtaatc
      721 ctatgaaatt tcctcaaaat tgtgattgta tgaatcaaaa aagtagcaat caatttgtat
      781 gtgaatatgg gataactata tataatatac caatactgta caaaagcaat agcataggtg
      841 ttataagagg agggtatgta ctattatctg atttgaattt agatacagag cataataatt
      901 tatatgatat tccagaggga gctgctagaa gtataaaacg cctcctgaaa caaatatcta
      961 aaaatataat aaatttttgc tcttttaatg atataagaaa ggatttacaa gaaaaagaga
     1021 agactatagc aagaacatat cattatggag agcaacttga aatgaattta aaagtagctc
     1081 aagatatggt tactaattta agaataaatc atcacttttt atttaataca ttaaattcaa
     1141 tggcaagcat agcgctagat gatggtagtt atgatttgta tagtgcaata atagatttat
     1201 caagaatgtt tagatataca atgaggtctg atttaagatt tgtagaatta gaatctgaaa
     1261 tattatacat taaaaattat ttgaatttac agaaattacg ttatggagat gcacttaaag
     1321 taaaatatct tattccagag aagttatata acctttctgt gccttttaac tttattcaac
     1381 ctattgtaga gaatgcattt acacatggat ttagagatat tgacacagaa aagcgaattg
     1441 aaataattgc aagattagat tctcagtatg ctattataga gattcataat aatggaacta
     1501 tcttagatgg aaataatata gacaaaataa aggcaggtat tcgtagtaat aatggtcatg
     1561 gtttgtcttt aatatataca aaattcacat ctgcttatgg aaataatttt gatatggata
     1621 taaagtcgag tgataatgaa ggaacttaca taatgattaa aattcctatt gaaaattata
     1681 aggaatatgt atagaacttg tgaaaaatta ataagtttta taaatgttaa gaaatatatt
     1741 agatgttttt atgttgtttt atatatctta cttttttata ttattaagct aaaagggagg
     1801 tattctcatg agaagagcac taataataga tgatgaagaa gctgttctaa aaataataac
     1861 acattttata gaaatgaaag atatgccaat aaaaattgtt ggtaaagcaa catctggtga
     1921 tgaagcagta gataaaatca ttggtttaga gccagacata gtttttatag atattcaaat
     1981 gccaatatac aacgggttag aagtaataga aaaaacatct catttatcta aaaaaataaa
     2041 ctttgtagta ataactgcat ttaactattt tgaatatgct caaaaagctt tgagattaaa
     2101 tgtaaaagat atactcctta aaccattgga tatgaaagaa tttactaagt ctgtagaaaa
     2161 aattattgga tatcaatata ccaataatga tttattaaat gagatactgg aatatataaa
     2221 tctaaactat agcaatgact taaagttaaa tgattgtgct agattatttt taaccaatcc
     2281 atctaatata tctaggattt ttaaaagtaa tttaaataca acttttatat catatctaaa
     2341 tcatataaga atagaaaaat ctattgagtt acttgaaaat actacaaaaa gtataaatga
     2401 gattgcagaa atagttggat ataatagcct aaataacttt tataaaaact ttaagataga
     2461 aaaaggaatg actccaaaag tttataaact gaacactaaa aattgaaatt gataaataaa
     2521 aagtcaatat agataattgt ttgagtgtgt ctgaataagt atactttaat tatgataaaa
     2581 attaaagcgc tttaaacata tttaataata ttaaacctta taaaagctgt acctatttat
     2641 ataaaaaaat atatataaat aaaaagaaaa agatatacaa aataaaaaaa gtgaattaaa
     2701 tactgtttta aaaatacaga attttcattt aattcactga gagtttatat ttcttaaagg
     2761 aaggtaactt tataatggag gtaatttaat atgtcagaca aagtgaatat gaaaaatatt
     2821 atctgtattg caggcgctta tctagcaaca gcaattggtt ctgggtttgc tactggtcaa
     2881 gaaattctac aattttttgc ctcacaaggg tatatgggtt taattggagc attaatatca
     2941 atggtattgt tttcatttct tggtgctgaa gttataacta gaggtagaga attaaaactt
     3001 aaagaaccaa caagaattta cactttttat tgtgggaaga ttttaggaaa attttacgaa
     3061 tggtttagtc caatattttt atttgggatt cttatagtaa tgatttcagg tgcaggctca
     3121 actttaactg aatactatgg tataaatcct tacttaggaa gagtattgat ggctgttgct
     3181 actttaatta cagtgtctat gggtttagaa aaattagtaa gcatacttgg acgtattgga
     3241 ccagtgatag cagtatttac aattgctgtt ggattaataa gcttgtttaa gcattttgac
     3301 ggtttatcta tggttggaga agctatgaaa aatattgaag ttacaaaagc atcagaaagt
     3361 ccaatcgttt ctggtgtaat atataataca ttaaatatta ttgtaatgat tgttttttta
     3421 acaggaatgg gagcttctat tgaaagtaaa aaggatgctt tttggggagg tttattaggt
     3481 ggaatagtgt ttatgactgc tggtatagtt atgtatttgg ctatgatttc agatatagga
     3541 aatttgtata tgaaagaaat accatcactt tatttagcag ataatatatc tccgattgta
     3601 ggtgtatgtt tttcagttgc attaatatta ggtatctata caacagctgt tccactttta
     3661 tggtctgtaa ccaatagaat tgttgaagat gaacatccta aatttaaatt ggtaacaaca
     3721 gtaatagcta ttttggcttg tataggaggt tttttaccgt ttggtaaatt agtaaacatt
     3781 ttatatccat atacaggtta tatggggctt ttaattttag tctgtatggt gtataaagca
     3841 attacatgta gaaaaaaatc aacacaaagt caggaataga gccacttatt aatagttttt
     3901 atttaaagaa attttttata taatccgaat ttaaaaag
//
LOCUS       DAELLV010000282         3934 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.282, whole genome shotgun sequence.
ACCESSION   DAELLV010000282 DAELLV010000000
VERSION     DAELLV010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.282"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..600
                     /locus_tag="KPD58_003359"
     CDS             <1..600
                     /locus_tag="KPD58_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HBG0267086.1"
                     /translation="YLIIAVIFIISVTMLILISHINILIIADIKNKDICLKLNIKYMF
                     NLININRQLYPAENSKNNDKKEGMKNNIDSSILLADDLLSIYRLLKKIKIHELYSNIN
                     FGTGNIGLTSSVYVLINTLYGNLFNMIDAEKMYLNVNPDFTKDYVLGNIRIHIRPRIK
                     ALFNIIIMINKIMNKNKGNKEGDSNESNRFDTESYGNNS"
     gene            554..982
                     /gene="ytfJ"
                     /locus_tag="KPD58_003360"
     CDS             554..982
                     /gene="ytfJ"
                     /locus_tag="KPD58_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HBG0267087.1"
                     /translation="MRATGSIQSLMETTLETIKGSIDANTIIGDPIKTDTTVVVPISK
                     VTIGFGIGGGEYSKGYDDKDREIELKNEKSDTNFAGGSAGAISVQPVAFVVVESGETR
                     IMSLDSNINLVDNILSITPRVLEKIQNISQSNKNQDKNNM"
     gene            1230..1670
                     /locus_tag="KPD58_003361"
     CDS             1230..1670
                     /locus_tag="KPD58_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0267088.1"
                     /translation="MSDKELSILLLESMNNFHNLIKIINNERYKKDKVLTERQFFALV
                     KIRKHDKIELKNLSRDLHVSTSSLCILLNKLVEQEYVYREEDSRDRRNTFYGITKNGE
                     KILDNEILKFVSIISDKMDCLDIDNKDKLFTSLEESKNIIEQLF"
     gene            1686..3137
                     /locus_tag="KPD58_003362"
     CDS             1686..3137
                     /locus_tag="KPD58_003362"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HBG0267089.1"
                     /translation="MNIKYSKEILKSLENAITVVALFEGEDVFTTFDKINMISRNFNS
                     KENSIDTVTLIENNNLVNVCFVGFGKRELLNREKIRIIGGNLCKKLKNILKNEAYKSS
                     DVNVLNLNLNSDEIGCFVEGILLGNYKFDKYKTKSKDFERKEVDTITIFTEKEVECQI
                     EKAKILANSTIIARNLVNEPSNVIYPKTLAVETVKLGAEFGFNVDVYEEEKIKALGMD
                     AFFAVSRGSINKPRFIVMRYFGDKESTDILGLVGKGLTYDTGGYSLKSNASMLDMKTD
                     MAGAASVIGAMCAISQSKLKKNVIAVVAACENALSGGSYKPGDIISSMAKKTIEVLNT
                     DAEGRLTLADAIYYIINNEKVTKVVDVATLTGAALTLLGNVATPIVTNNDDFYCELEK
                     AATLSGERVWKMPIYDEFKDMIKGEEADLKNTGGKNAGCITAGAFIGEFVGNTPWIHM
                     DIAGTSTSSKSIGYKAKGATGEPVRTLYYLAEI"
     gene            complement(3228..3896)
                     /locus_tag="KPD58_003363"
     CDS             complement(3228..3896)
                     /locus_tag="KPD58_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HBG0267090.1"
                     /translation="MFKIFLFSSEQFVSLFIFGLFLYYCPKLTKNILPYSYTVEKIIC
                     TLLVIIMALEQLLLISSGNYSTLNSLPIGINYICIYLCIAILIFKQYHLFNIFFSWSL
                     VCSVGELIFSKNLGYEFPSLIYFIFIFSKCLIIYADIYMVDVRKFRVNRYALRDNLAI
                     CFIYFSFIFLLNTFTNSRYYYGFLSHSTTAIFTFIFVTSIMYIPALLFNRDTFILEKK
                     KKSK"
BASE COUNT         1643 a          355 c          672 g         1264 t
ORIGIN      
        1 tatttaataa tagctgtaat ttttattata agtgtaacta tgttgatttt aatatctcat
       61 ataaacatct taataatagc tgatataaaa aacaaagata tttgtttaaa gcttaatata
      121 aaatacatgt ttaatttaat aaatataaat agacaacttt atccagctga aaatagtaaa
      181 aataatgata aaaaagaggg gatgaagaat aatatagatt caagtatatt attagctgat
      241 gaccttttga gcatttatag attattaaaa aagataaaaa tacatgaact ttattcaaat
      301 attaattttg gaactggaaa tattggctta acatcttctg tatatgtatt gataaatact
      361 ttgtatggaa atttatttaa tatgattgat gcagaaaaaa tgtatttaaa tgtaaatcca
      421 gattttacaa aagattatgt tttaggaaat ataaggatac atataaggcc aagaataaaa
      481 gctttattta atattataat aatgattaat aaaattatga ataaaaataa aggaaataag
      541 gaaggtgata gtaatgagag caacaggttc gatacagagt cttatggaaa caactcttga
      601 aacaattaaa ggttctatag atgcaaatac tattatagga gaccctataa aaacagatac
      661 tacagtggtt gtacctatat caaaggttac aattggcttt ggtataggag gaggagaata
      721 ctcaaaagga tatgatgata aagataggga aattgaatta aaaaatgaaa aatcagatac
      781 gaattttgct ggaggtagcg caggggctat atctgtacaa ccagtagcat ttgtagtagt
      841 tgaaagtgga gagactagaa taatgagttt agatagtaat atcaatctag tggataatat
      901 attatctata actcctagag tattggagaa aattcaaaat atatcacaaa gtaataagaa
      961 tcaagataaa aacaatatgt aagaataaaa tatttttaaa tatgtatata gttttaagta
     1021 aagtagtggg gttaatataa atcccactat tttgttgtgt actttaaaca tttatatcta
     1081 ataagatttt agtttaaaac aatttaaata gataataaag ttgatatata gtataattat
     1141 aaaaagaaaa gaagtattat attatagata tataaacgca tttgagatat aagagggagt
     1201 tataaatcat tagttttgag gtgaatgtta tgagcgataa agaattatct attttgttgt
     1261 tagaaagtat gaataatttt cataatttaa taaagattat taataacgaa agatataaaa
     1321 aagataaggt tcttacagaa agacagttct ttgccttagt aaaaataaga aaacatgata
     1381 aaatagagct taaaaatcta agtagagatt tgcatgtatc tacttctagt ctgtgtatac
     1441 tacttaataa attggttgag caagagtatg tatataggga agaagatagt agagatagac
     1501 gaaatacttt ttatggaata actaaaaatg gagaaaagat attggataat gaaatactaa
     1561 agtttgtatc tataataagt gataaaatgg attgcctaga tatagataat aaagataaat
     1621 tatttacttc tttggaagag tctaaaaata taatagaaca attattttaa aaaggagaat
     1681 ttattatgaa tataaagtat tctaaagaga tattaaaaag tttagaaaat gctataacag
     1741 tagtagctct atttgaggga gaagatgttt ttacgacatt tgataaaata aatatgataa
     1801 gcagaaattt taattctaaa gaaaatagta tagatactgt aacattaatt gaaaataaca
     1861 atttggtaaa tgtttgtttt gttggttttg gtaagagaga gttattgaat agagaaaaaa
     1921 ttagaatcat aggtgggaat ttgtgtaaaa aattaaaaaa tatattgaaa aatgaagcat
     1981 ataagagtag tgatgttaat gtactgaatc ttaatttaaa tagtgatgaa ataggttgtt
     2041 ttgtagaagg gatattactt ggaaattata agtttgataa gtataagaca aaaagtaagg
     2101 attttgaaag aaaagaagtt gatactataa ctatatttac tgaaaaagaa gttgagtgtc
     2161 aaatagaaaa ggctaaaata ttagctaatt caactataat agctagaaat ctagttaatg
     2221 aaccatcaaa cgtaatatat ccaaaaacat tagcagtaga aacagttaaa cttggtgcag
     2281 agtttgggtt taatgttgat gtttatgaag aagaaaaaat aaaggcattg ggaatggatg
     2341 cttttttcgc tgtttcaaga ggttcaatta ataaaccaag attcatagta atgagatact
     2401 ttggagataa agaaagtaca gatatattag gtctagtagg taaaggattg acttatgata
     2461 ctggtggata ttctttaaaa tcaaatgctt ctatgctaga tatgaagaca gatatggctg
     2521 gtgctgcgag tgttataggt gctatgtgtg ctatatctca aagtaaatta aagaaaaatg
     2581 ttatagcagt agtggctgca tgtgagaatg ctttatcagg aggttcatac aaacctggag
     2641 atataattag ttctatggca aagaaaacta ttgaagtttt aaatacggat gcagaaggta
     2701 gacttacatt agctgatgca atatactaca ttataaacaa tgaaaaagta acaaaggtgg
     2761 ttgatgtagc tacattaact ggtgctgctt taacattgtt aggtaatgtt gcaactccta
     2821 tagtaacaaa taatgatgat ttttattgtg aattagaaaa agctgccact ttatcagggg
     2881 aaagggtatg gaagatgcca atttatgatg aatttaaaga tatgataaaa ggagaagaag
     2941 cagaccttaa aaacactggt ggtaaaaatg ctggatgtat aacagcagga gcttttattg
     3001 gtgaatttgt tgggaatact ccttggatac atatggatat agcggggaca agtacatcat
     3061 caaaatcaat tggatacaaa gcaaaggggg ctacaggaga acctgttaga acattgtact
     3121 atttggcaga aatctaaaaa acaattataa aatataattt ataaagccac gtggatattt
     3181 ttgtaattta agattatcta tgtggcttta ttttttattt aagtaaatta tttactcttt
     3241 ttcttttttt caagtataaa agtatctcta ttaaataaaa gagcaggtat gtacataata
     3301 ctagtaacaa aaataaacgt aaaaatagca gttgtgctat gacttaaaaa accataatag
     3361 tacctagagt tggtaaatgt gtttagtaaa aatatgaatg aaaaataaat aaaacatata
     3421 gctaagttat ctctaagtgc atatctgtta actctaaatt ttctaacatc aaccatatat
     3481 atatctgcat aaattatcaa gcacttagag aagataaaga taaaatatat taagctagga
     3541 aattcatagc ccaagttttt agaaaaaatg agttctccaa ctgaacaaac taaactccaa
     3601 gaaaaaaata tattaaaaag atgatactgc ttaaaaatta gtatggctat acacagatat
     3661 atacaaatat aattgattcc aattggtaaa gagtttagag tactataatt acctgaagaa
     3721 ataagtaata attgctctag agccataatt attacaagta atgtacaaat tatcttctca
     3781 actgtatagc tataaggtaa aatatttttg gttagttttg gacaatagta taaaaataat
     3841 ccaaatataa agagacttac aaattgttca ctagaaaaaa gaaaaatttt aaacatataa
     3901 cattctctcc tataataaat ttaatatgta tcta
//
LOCUS       DAELLV010000283         3919 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.283, whole genome shotgun sequence.
ACCESSION   DAELLV010000283 DAELLV010000000
VERSION     DAELLV010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.283"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            129..1784
                     /gene="ilvD"
                     /locus_tag="KPD58_003364"
     CDS             129..1784
                     /gene="ilvD"
                     /locus_tag="KPD58_003364"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HBG0267091.1"
                     /translation="MRSDIKKGIEGAPKRALMYGMGLTKEEIERPLIGIVNAQNEVIP
                     GHLHLDEIAEAAKNGVRMSGGLPLEFPAIGVCDGIAMGHVGMNYSLASRELIADSIEA
                     MAMAHGFDALVLIPNCDKIVPGMLMAAARLNIPSIVVSGGPMLPGKKNGKVYDFNSAM
                     EGVGACKDGTVSEEELEALAMNSCPGCGSCSGLFTANSMNCLTEALGMGIPYNGTAAS
                     HSGERKRIAKYAGMYVMELLKNDIKPRDILTIDAFKNAIAVDMAMAGSTNTVLHLPAI
                     AYESGIELNLDFFDEISEKTPCLTKLSPSGKHHIEDLHMAGGIPAIMNELSKINGINL
                     DCKTVTGKTIGENIRNCEIENEEVIHTLKNPYSNQGGLAILKGNLALNGAVVKKSAVA
                     EEMLVHEGPARVFNSEEEAVNAIFGKKINKGDVIVIRYEGPKGGPGMKEMLSPTSAVA
                     GMGLDKYVALLTDGRFSGATRGASIGHISPEAMEGGLIGLVEEGDIISINIPDKKLEL
                     KVDEVEIENRKLKFKPLEPKIKHGYLSRYAKLVTSANTGAVLK"
     gene            2128..2760
                     /locus_tag="KPD58_003365"
     CDS             2128..2760
                     /locus_tag="KPD58_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0267092.1"
                     /translation="MNREEKSKNSKEKIIQSAFSLFSSKGYDSTSTQDIINLSGLSRG
                     AMYHHFKTKEDILRSVTKELYSQMNNFLEHLVADDTLTANEKIIELVVHSANDYTRRK
                     MVHCSWLEKIPFALIEEVRNLNNVVAPNIAKIIKQGVENKEFSCEYPEELAEMLVFSI
                     DILLDPVLFKREYSEVCNRLDFLLFMLKKMDIPLIDEYGIQKFKDLFRQL"
     gene            2783..3595
                     /locus_tag="KPD58_003366"
     CDS             2783..3595
                     /locus_tag="KPD58_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0267093.1"
                     /translation="MFYNAKSCQMMIGSNTVNYIEFGSGKKTLIILPGLGDGLFPLHG
                     KIQAIAFAFRYKQFTKDYKVYVFSRKNQITEKYSTRDMAKDQADIMKKLGIMKAEVMG
                     VSQGGMIAQYLAIDYPELVEKLVLAVTSSKQNDTIQNVICSWINMAKKQNYNDLMIDT
                     AKKSYSERYLKKYQLFIPFLGKVGKPKDFKRFIIQATSCIEHNAFSELNKITCPTLII
                     GGANDKIVGNNASFHLVEKIKKSEIFIYEGLGHATYEEAQDFNERVLEFLNK"
BASE COUNT         1520 a          446 c          732 g         1221 t
ORIGIN      
        1 ataaaaaatc gtaataaatt ttcattagta tatgctaata tttttttaca attttcgtaa
       61 ttaattaata gaaaaatata ttataataat attatgaaaa tttaaaaaag taagaaagag
      121 gtaatattat gagaagtgat ataaaaaaag gaatagaagg agctccaaag agagcgttaa
      181 tgtatggaat gggtcttact aaagaagaaa ttgaaagacc attaataggt atagtaaatg
      241 cacaaaatga ggtgattcct ggtcatctgc atcttgatga aatagcagaa gctgcaaaaa
      301 atggagtaag aatgagtggt ggtttgccat tagaatttcc tgctataggg gtatgtgatg
      361 gtatagctat ggggcatgtc ggaatgaatt attccctggc ttcaagagaa ttaatagcag
      421 attcaataga agctatggct atggcacatg gttttgatgc tttggtactt atacctaatt
      481 gtgacaagat agtgccaggt atgttaatgg ctgctgcaag acttaatata ccaagtattg
      541 ttgtgagtgg agggcctatg cttccaggta aaaaaaatgg aaaagtatat gattttaact
      601 ctgcaatgga gggtgttgga gcatgtaaag atggaacagt aagtgaagaa gaattagaag
      661 cgttagccat gaactcttgt cctggatgtg gttcatgttc tggtcttttc acagcaaata
      721 gcatgaattg tttaacagaa gctttaggca tgggtatacc atataatggt actgctgcgt
      781 cacattctgg agaaagaaaa aggatagcaa aatatgcagg tatgtatgtt atggagttac
      841 ttaagaacga cataaaacct agagatattt taacaataga tgcttttaaa aatgctatag
      901 ctgtggatat ggcaatggct ggctctacaa atacagtact tcacttacct gcaatagctt
      961 atgaatcagg aatagagctt aacttagatt tttttgatga aataagtgaa aaaactcctt
     1021 gtttaacaaa attaagtcca agtggaaaac atcatattga agatttacat atggcaggag
     1081 gaataccagc tataatgaac gaactttcaa agataaatgg aataaattta gattgcaaaa
     1141 ccgtaacagg caagactata ggggaaaata taagaaattg tgaaatagaa aatgaagaag
     1201 taatacatac attaaagaat ccatatagta accaaggtgg acttgcaata ttgaaaggaa
     1261 atcttgctct aaatggagct gttgtaaaaa agtcagcagt tgcagaagaa atgttagttc
     1321 atgaaggacc agcaagagtt tttaattcag aagaagaagc tgtaaatgct atttttggta
     1381 aaaaaataaa caaaggtgat gttatagtta taagatatga aggtccaaag ggtggtccag
     1441 gaatgaaaga aatgctatct cctacatcag cagttgcagg aatgggactt gataagtatg
     1501 tagcacttct tactgatggt cgtttttcag gagcaactag aggagcatct ataggccata
     1561 tttctccaga agctatggaa ggtggtttaa tcgggttagt tgaagaagga gatataattt
     1621 ctataaacat accagacaaa aaattagagt taaaagtaga tgaagttgaa atagaaaata
     1681 gaaaattaaa atttaaacct ttagaaccga aaataaagca tggatactta agtagatatg
     1741 ctaaactggt aacatcagca aatacaggag cagttttaaa atagtagact atatttatgt
     1801 aaattagaaa aagttaagaa aatattaatt ggatatctta ggtgttatag cactaatgag
     1861 gtatcctttt tattttgtat gaaaaacaca gcaaaatctt gattatcttt gaaaacaata
     1921 catctctatt ataaggaata cgagtacagc atagagatgt atcttttaga ccttgttgag
     1981 ggaatacaac tttttaaaag tttgatgtgg aatgcttttc gttccatcta aactatgaag
     2041 aaattgaaag tcagttaatt cttgacataa taaatattaa aatgtataat gaaaaacaaa
     2101 cagacagtct gtttttagga gggaaatatg aatagagaag aaaaaagtaa aaatagtaaa
     2161 gaaaaaatta tccaatcagc attttcacta ttttcgtcta aaggatatga ttcaacatct
     2221 acacaagata ttatcaattt atctggtcta tctagaggtg caatgtatca tcactttaaa
     2281 actaaagaag atatactgag aagtgtcaca aaagaacttt actcacaaat gaataatttt
     2341 ttagagcatc ttgttgctga tgacaccctt acagcaaatg aaaaaataat agaattggtt
     2401 gttcatagtg cgaatgatta cacacgtaga aaaatggtac attgtagctg gttagaaaaa
     2461 atcccattcg ctttaataga ggaagttcgt aatctcaaca atgtagttgc acccaatatt
     2521 gctaagataa ttaaacaagg tgttgaaaat aaagaatttt cttgtgaata tccagaggaa
     2581 ttagctgaaa tgctcgtttt tagtattgat attttacttg accctgtatt atttaaacgt
     2641 gaatacagtg aagtatgtaa taggttggat tttttattat tcatgttaaa aaaaatggat
     2701 attcctctaa ttgatgagta tggtattcaa aaatttaaag atttatttag acaattataa
     2761 taggaaaagc gaggaataga ctatgtttta taatgcaaaa agttgtcaaa tgatgatagg
     2821 aagtaataca gtgaattata ttgaatttgg tagtggtaag aagacactaa ttattttacc
     2881 aggattagga gatggtttat ttcctttaca tggaaaaata caggcgattg cttttgcttt
     2941 tagatataag caatttacaa aagattataa ggtttatgta tttagtagaa aaaatcaaat
     3001 tacagaaaaa tattccacaa gggatatggc taaagaccaa gcagatataa tgaaaaaact
     3061 tggaattatg aaagcagaag taatgggtgt ttctcaaggt ggaatgatag ctcaatatct
     3121 ggcaattgat tatcctgagt tagttgagaa acttgtgttg gcagttactt cttcaaagca
     3181 aaatgatact atccaaaatg ttatttgtag ttggattaat atggcgaaga agcagaatta
     3241 caatgattta atgattgaca cagcaaagaa atcctattct gaaagatatt taaaaaaata
     3301 ccaattattt attccttttt taggaaaagt aggtaaacca aaggatttta aacgatttat
     3361 tattcaagcg acctcttgca tagaacataa tgccttttcc gaattaaata aaataacatg
     3421 tccaacctta attataggag gtgctaatga taaaattgtt ggaaataatg cttcttttca
     3481 tttagtagaa aaaattaaaa aaagcgaaat atttatctat gagggattgg gacacgctac
     3541 atatgaagaa gcacaggatt ttaatgaaag agttttagag tttttaaata agtaaacacc
     3601 tttgttaaaa atatacatat gaagattgga aatttaatgt taaaaataga aacatgaaaa
     3661 tatgcttaac tggtattttt actattcata accaattttt gatacattat ctactataaa
     3721 tacaaatata gcttcaatgt gattatatat tgttgtattg gtaaagcact tatacaaaca
     3781 gaggaatttt gtaaattcat attataccca catttgttaa cttatgaaaa tataatcaaa
     3841 atttttatga gctttttata taaaaaatat aatgtttgta tacaaaaata ttaaagacat
     3901 tttagtaagt tttgtatat
//
LOCUS       DAELLV010000284         3911 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.284, whole genome shotgun sequence.
ACCESSION   DAELLV010000284 DAELLV010000000
VERSION     DAELLV010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3911
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.284"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(118..1035)
                     /locus_tag="KPD58_003367"
     CDS             complement(118..1035)
                     /locus_tag="KPD58_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HBG0267094.1"
                     /translation="MVNMNIIEIRSDKIYKKIMDAPINKKEDIYRYELMKPFEFKWKC
                     MNVPIVARQKGGYDVIIASEMLGVLSPKDIDEKQKKNINVLSADKIWGTCKETIENSI
                     NAFIKEGYDLNIKDYKYSILLANPNSPYTILSDGYWGDGGIPGYIFLSLVPNEYTINR
                     LPVLIAHECNHNIRFQFIEWNNNITLEEMMINEGLAENFATWMFGEEMLGPWVSRTDI
                     ETLNTYIKPIIKSALKETGFRNITSYLYGDDIAKMQGYFPVGLPYCAGYACGYYMIKY
                     YLEKTNKSIIEATLLPYSEIIEAVKEFWE"
     gene            1297..1674
                     /locus_tag="KPD58_003368"
     CDS             1297..1674
                     /locus_tag="KPD58_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG0267095.1"
                     /translation="MLTPYLIFNGTCEKAFNFYAEAFGGGKTIFARLDSNPNNPIMHA
                     SVTFTKYEGCIMGADTDKPVVISGMAICVVLPSREAIEEISVKLAEGGTLVQEFLPHP
                     PPHQNDGAAEVLDRYGYTWYLST"
     gene            complement(1830..2279)
                     /locus_tag="KPD58_003369"
     CDS             complement(1830..2279)
                     /locus_tag="KPD58_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0267096.1"
                     /translation="MKSGIILEMKQINSTEYDEMLQVWESSVRATHDFLTEKDIESLI
                     PLVEIGLKEVENIVCIKDNDIIKGFIGIDKDKIEMLFIEDKYRGNGIGKKLIKYAIDK
                     YNVKYVDVNEQNKKAVGFYIHLGFKVFDRSEIDGQGNPFPILHMKLI"
     gene            complement(2443..2847)
                     /locus_tag="KPD58_003370"
     CDS             complement(2443..2847)
                     /locus_tag="KPD58_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HBG0267097.1"
                     /translation="MSYQNYKCERDYVYDDYKYNKCNKCYNDYEEMTHVHEYSESVKL
                     AEECEDRHNHRAAGVTGEAIPINGGTNHVHKINDNVDFLDHFHKICVTTGPAIRIPGT
                     DKHIHLICGETTVNDGHCHKFLFTTQIEAPLV"
     gene            complement(2960..3826)
                     /locus_tag="KPD58_003371"
     CDS             complement(2960..3826)
                     /locus_tag="KPD58_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBG0267098.1"
                     /translation="MIDIHKNIYDNKLFEELKIDCKKCFGLCCVALYFSASDGFPIDK
                     ESGKPCINLQPDFKCSVHNSLMKRGFKGCTAYDCFGSGQKVAQVTYKGIDWMQSPELT
                     NQMSEVFLIMRQLHEMLWYLKEASVLNISDTIKSKIDLIIEETEKITNMGPEQIINLD
                     IISHRTKVNLLLSQASESVMGKVKSFIKTSTLKNMKKLSKNIDLIGADLRKINLIGAD
                     LRGRFLIAANLRNTDLSGANLIGADLRDCDIRGANLENSIFLTQLQVNTAKGDSSTKL
                     PASLIRPKYWEK"
BASE COUNT         1272 a          667 c          431 g         1541 t
ORIGIN      
        1 attgaaatat ttctttatat atattattgt atatttctta taaattattg tcaatttatt
       61 ttttcttaaa aataaaataa ttgcataatt tatttcaaat tatgcaattt ataaaattta
      121 ttcccaaaac tcttttactg cttcgattat ctcactataa ggcaataaag tcgcttcgat
      181 tattgattta tttgtctttt ctaaataata cttaatcata taatatccac aagcatatcc
      241 tgcacaataa ggcaacccta ctggaaaata tccttgcatt ttagctatat catcaccata
      301 aagataagat gttatatttc gaaatccagt ttcttttaaa gcacttttta ttattggctt
      361 tatataagta tttaatgttt cgatatctgt tctactgacc caaggtccta acatttcctc
      421 tccaaacatc catgttgcaa aattttctgc aagaccttca tttatcatca tttcttctaa
      481 tgttatatta ttattccact ctataaactg aaatctaata ttgtgattac attcatgtgc
      541 tattaatact ggtaatctat tgatagtata ttcattagga accaatgata gaaatatata
      601 tccaggaatc ccaccatcac cccagtatcc atcacttaat attgtataag gactatttgg
      661 attcgccaat aatattgaat atttatagtc cttaatgttt aaatcatacc cttcttttat
      721 aaaagcattt atagagtttt ctatggtttc tttgcaagtg ccccaaattt tatcagcaga
      781 taacacattt atattctttt tttgcttttc atcaatatcc ttaggcgata aaacccctaa
      841 catttcactt gctataatta catcatatcc acctttctgt ctagcaacta ttggaacatt
      901 catacacttc cacttaaatt caaaaggctt catcaattca tatctgtata tatcttcttt
      961 tttgtttatt ggtgcatcca ttatcttctt gtatatttta tctgagcgaa tttctataat
     1021 attcatattt accatctcct ttaagacaaa gtatatacaa ttacgtagcg tcaatgtcaa
     1081 tatacatttt ttataatgtc accttaatct ttttattata ttttatcctg taaaccttcc
     1141 gtacatataa cattttttta aattaataaa tttgtataat ataataataa aaaattatat
     1201 acttcaaggt ttccctatga tacaatttac taaatcataa tttagattat caggtgcaac
     1261 tataaattat tctatatcaa ggaggatata ttggtcatgc taactccata tttaatattt
     1321 aatggtactt gtgaaaaagc atttaatttt tatgctgagg ctttcggagg aggaaaaact
     1381 atatttgcgc gattagacag caatccaaac aatcctatta tgcacgcaag tgttactttc
     1441 acaaaatatg aaggttgtat aatgggtgcg gatacagaca agcctgttgt aatttctggc
     1501 atggcgattt gtgttgttct accatctcga gaagcgatag aagaaatatc tgtaaaactt
     1561 gccgaaggtg gtacacttgt acaagaattt ttaccacacc caccaccaca tcaaaatgat
     1621 ggcgctgctg aagtacttga taggtatggg tatacttggt atttaagtac atagttaaaa
     1681 taaaaaaact acatctaata atcataatat agaacagata aatttcaatt taatagtgta
     1741 gtaaaaaagt cgtgtcctaa gactgtcacg acttttattt gtataaaaat ttaagtaaat
     1801 atttttgcga ataaatgaga agtataactt taaattaatt tcatatgtag tattggaaat
     1861 gggttacctt gtccatctat ttcagaccta tcaaacactt taaatcctaa gtgaatataa
     1921 aatcctacag cttttttatt ctgctcattt acatcaacat attttacatt gtatttatct
     1981 atagcatatt ttattaattt tttaccaata ccatttcctc tatatttatc ttctataaag
     2041 agcatctcaa ttttatcttt atctattcct ataaagccct ttattatatc attatctttt
     2101 atacaaacta tattttctac ttctttaagt ccaatctcaa ctaatggtat caaagattca
     2161 atatcttttt ctgttaaaaa atcatgcgtt gctcttactg atgattccca gacttgcaac
     2221 atttcgtcat attctgttga atttatttgt ttcatttcaa gaattattcc agatttcata
     2281 aaaatttcct ccctatatca cttttaattg cataacactt ttttatacaa aacttagaaa
     2341 ggactacgaa ggttaaatat catagtcctc attaagttta aatattatct attatatatt
     2401 atactataat ctagcaaatt cactacaata attcacatca gcttatacta aaggtgcttc
     2461 tatttgagta gtaaaaagga atttatgaca atgaccatca ttaacagtag tttctccaca
     2521 tattaagtga atgtgtttat cagttccagg tattctaatt gctggccctg ttgtaacaca
     2581 tatcttatgg aaatggtcaa ggaaatctac attatcattt attttatgaa catgatttgt
     2641 acctccattt attggtatag cttcaccagt aacccctgct gcacggtggt tatgtctatc
     2701 ctcacattct tctgctaact taacactttc tgaatattca tgaacatgtg tcatttcctc
     2761 ataatcatta tagcacttgt tgcacttatt gtatttataa tcatcataca cataatctct
     2821 ttcacattta taattttgat aactcatttt tatatctctc cctatattat attaagaaat
     2881 tcttgtaaag aatctctaat gtataatatg aattaaagtc aaaaaagttc atattttgta
     2941 aaattatgta ttaatttaat tatttttccc agtatttagg gcgtattagt gaagctggta
     3001 acttcgtact agaatctcct tttgcagtat ttacttgtaa ttgtgttaaa aatatactat
     3061 tttcaagatt agcacctcta atatcacaat ctcttaagtc tgctcctatt aaatttgccc
     3121 cactcaaatc tgtatttctt aaatttgctg ctattagaaa tctacctctt aaatctgctc
     3181 ctattagatt tatttttcta aggtctgctc ctattaaatc tatattttta cttaattttt
     3241 tcatgttttt aagagtagaa gtcttgataa atgatttaac ctttcccatc acagactcac
     3301 tcgcttgtga taataataaa ttaacctttg ttctatgtga tatgatatcc aaattaatta
     3361 tctgttctgg gcccatatta gttatctttt cagtttcttc aattattaaa tcaatttttg
     3421 atttgattgt atctgatata tttaatacag atgcttcttt aagataccat aacatttcat
     3481 gtaattgtct cataattaaa aacacttcag acatttgatt tgttagttct ggtgattgca
     3541 tccagtctat ccccttatat gttacttgtg ctactttttg acctgaacca aaacaatcat
     3601 atgcagtaca ccctttaaaa cctcgcttca taagactatt atgtacagaa catttaaaat
     3661 ctggttgtaa attaatacaa ggttttcctg attccttatc gataggaaat ccatctgaag
     3721 cagaaaaata aagagctaca cagcaaagtc caaagcactt cttacagtca atctttagct
     3781 cctcaaataa cttattatca tatatatttt tatgtatatc tatcatattt tttctcctct
     3841 taaactttat ttttaatatt atattatcta taactaaatg ataatcaagt cccttattta
     3901 acagttctta t
//
LOCUS       DAELLV010000285         3818 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.285, whole genome shotgun sequence.
ACCESSION   DAELLV010000285 DAELLV010000000
VERSION     DAELLV010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.285"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(130..525)
                     /locus_tag="KPD58_003372"
     CDS             complement(130..525)
                     /locus_tag="KPD58_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBG0267099.1"
                     /translation="MKALEFLNKARVYYLATVDENNQAHVRPLGFVMEYNGKLTFCTS
                     NQKDMFKQLIFNPKVELCCIDQNLNTLRILGEAVFVTSKETQAKALEIMPSLGQMYSV
                     GDGKFEIFSIDKAKVSCCSMSGKKIDVEL"
     gene            666..1058
                     /locus_tag="KPD58_003373"
     CDS             666..1058
                     /locus_tag="KPD58_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0267100.1"
                     /translation="MSINTEKNITKNVEELTCPIRYALDIVGGKWKLPIICMLAVENP
                     IRYSSIKRKLDGITNTMLAQSLKDLESTGIVHRKQYNEIPPKVEYTLTSKGKSIVPIL
                     QQFANWGATNMQEKNTCGLSCKECRKIK"
     gene            complement(1158..1574)
                     /locus_tag="KPD58_003374"
     CDS             complement(1158..1574)
                     /locus_tag="KPD58_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBG0267101.1"
                     /translation="MNWQDAFFQDTKPKFEQINEFINNPLWDKLNKTLVSTYNVEPKL
                     EYSKCSMQRGWNVKYKKRGKSLCTLYPQEGYFKALVIVSESNRVEVDLFINTCCDYIK
                     KIYNEVNFFNGSKWLMIQVDSLLVLNDMLELIKFRA"
     gene            complement(1589..2500)
                     /locus_tag="KPD58_003375"
     CDS             complement(1589..2500)
                     /locus_tag="KPD58_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG0267102.1"
                     /translation="MHAWESIQITLDLIEANLSEEISINELANKANLSPFYYQRLFKR
                     LVNKTVMEYIKLRRLARASEYLAEHKNRILDVALNFGFASHETFTRSFKKAYGLTPEK
                     YRANPVKLNHFIKPELILNYAMVDEGVPLIADDIVIEVNRKTLSNPRTFVGIEIEVPI
                     CQLVGGETTGIAIVEELWMKLGSQRHNIPNQIPGGNEFAALYMGNAKEGNCMYMAGVE
                     VESGTSVEGYSTFELPAKEYLVCGFEAENFNELVNSAVFKADKFMERWMKKHNLTTTD
                     FAIEMYYPTTPEAAYLEHWIVPVPIEQ"
     gene            complement(2682..3704)
                     /gene="splB"
                     /locus_tag="KPD58_003376"
     CDS             complement(2682..3704)
                     /gene="splB"
                     /locus_tag="KPD58_003376"
                     /EC_number="4.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HBG0267103.1"
                     /translation="MFNPKRIIFEKGTLDTDVGKKIYNKFRDNKDVEIINISSNRIKE
                     HIPGENLYEQYRSGKQTMVVGIRKSLKFQTCKPSANYQLPLVSGCMGRCEYCYLNTQL
                     GDKPFIRVFVNVDEILEQTKKYIEDRKPQVTIFEGAATSDPVPVEPYTNSLKKTIEFF
                     GREEKGRFRFVTKYSDVDSLLDAEHNNHTEIRFSINTPRIINEHEHYTASANKRIESA
                     VKLARAGYQIGFIIAPVFLYEGWKEEYKNLIISIKEALPKDFDKKIIFEVISHRYTTK
                     AKNRILEIYPDTLLPMENEDRKFKYGQFGYGKYVYDKEQLDDMKEFFLKEINGVFKNS
                     LIKYII"
BASE COUNT         1271 a          656 c          432 g         1459 t
ORIGIN      
        1 catctaataa ctaaaaattt ttacatattc attatttaaa aatcttttta ttgcatttaa
       61 attaaatcta tcaacatttc agtaataacg ttttcttaat tttcactatg gatgtcgatt
      121 caattataat taaagctcta catcaatctt tttaccactc attgaacaac aactaacttt
      181 tgccttatca atagagaata tttcaaactt accatctcct acagagtaca tctgacctaa
      241 acttggcatt atttcaagtg ccttagcttg tgtttctttt gatgtaacaa atactgcttc
      301 acctaaaata cgtagagtat taaggttttg gtctatacaa caaagttcaa cctttggatt
      361 aaaaataagt tgcttaaaca tatccttttg attagatgtg caaaatgtaa gtttaccatt
      421 gtattccata acaaatccta aaggacgcac atgtgcttga ttgttctcat caactgttgc
      481 taaataataa actcgtgcct tgttcaaaaa ttctaatgct ttcataattc ttacacccct
      541 tttaaatact tgtattaagg attatccttt gatataattt taatataaaa tacagaaaaa
      601 acaagtatgc agttttttct gataaagtat aaaaaagata ctactataac taaggagtaa
      661 ttaaaatgag tataaatact gaaaaaaata taactaaaaa cgttgaagaa ttaacctgtc
      721 caatccgtta tgcattagac attgtcggtg gtaagtggaa attacctatt atatgtatgt
      781 tagcagttga aaatccaatt agatatagta gcataaaaag aaaattggat ggcattacaa
      841 atactatgct agctcaatca ttaaaagatt tagagtctac tggtattgtt catcgtaaac
      901 aatataatga aattcctcca aaagttgaat atacattaac aagtaaaggt aaaagtattg
      961 tgcctatttt acaacaattt gctaattggg gtgctactaa tatgcaagaa aaaaacacat
     1021 gtggacttag ttgcaaagaa tgtagaaaga taaagtaaat ttaaatttac taaattattc
     1081 atattataga ctatatatta atttatctga ttgaatataa aaacaggatg acttatatat
     1141 tcatcctgtt ctatatgtta agctctaaat ttaattagtt caagcatatc gtttaatacc
     1201 aataaactat ctacttgaat cattagccat ttacttccat taaaaaagtt tacctcatta
     1261 tatatttttt tgatataatc acaacaagta ttaataaata aatcaacctc tactctattg
     1321 ctttcactta caataaccaa agctttaaaa taaccttctt gtggatataa agtacatagt
     1381 gatttacctc tttttttata ttttacattc caacctcttt gcattgaaca tttactgtat
     1441 tcaagcttag gttctacatt ataagtagaa accaaagttt tatttaactt gtcccataat
     1501 ggattattaa taaattcatt aatttgctca aatttaggtt tagtatcttg aaaaaacgca
     1561 tcttgccaat tcatattcat catcctttct attgttcaat aggaaccgga acaatccaat
     1621 gttccagata tgctgcttca ggtgtagtgg ggtaatacat ctcaattgca aaatcagtag
     1681 ttgttaaatt atgtttcttc atccatcttt ccataaattt atctgcttta aaaacagctg
     1741 aattaactaa ctcattaaag ttttctgcct caaatccaca tactaaatat tctttagctg
     1801 gcaactcaaa agtactatat ccttctactg atgtacctga ctctacctct acacctgcca
     1861 tatacataca atttccttct tttgcatttc ccatataaag agcagcaaat tcatttccac
     1921 ctggaatttg atttggtata ttatgtcttt gacttccaag tttcatccat aattcctcta
     1981 ctattgcaat tccagttgtt tcaccgccca ccaactggca aataggtact tctatttcta
     2041 ttccaacaaa tgtccttgga ttactcaatg tcttacgatt gacttcaatc actatatcat
     2101 ctgcaatcag tggaacacct tcatcaacca tagcataatt taaaattaat tctggcttaa
     2161 taaagtgatt gagttttact ggatttgctc tatatttttc tggagttaat ccatatgctt
     2221 ttttaaagga acgtgtaaat gtctcatgac tagcaaatcc aaaatttaaa gctacatcaa
     2281 gtatacgatt cttatgttct gctaagtatt cagaagctct tgcaagacga cgaagcttta
     2341 tatactccat tacagtctta tttaccaatc ttttaaatag tctttgatag taaaatggtg
     2401 ataaatttgc tttatttgct aattcattaa ttgatatttc ttctgataaa tttgcttcta
     2461 ttaaatccaa tgtaatttga atagactccc atgcatgcat actataactc ctcctgatat
     2521 ttttgttact cttatccttt atagtatcaa aactagaaaa aatgttcttg atagatagtg
     2581 ccctttttaa ttttcttata tgatagtgct aaatctcatt gctttattat aaacataaaa
     2641 tgtataaaaa taaaatatag caaatcttta ggtaagattt gctatattat atattttatt
     2701 aaactatttt taaatactcc atttatttcc ttaagaaaga actctttcat atcatctaat
     2761 tgttctttat catacacgta cttaccatat ccaaattgac cgtatttaaa ctttctatcc
     2821 tcattttcca ttggcaatag tgtatctgga taaatctcta gtattctatt ttttgcttta
     2881 gttgtatatc tatgagatat aacttcaaat ataatttttt tatcaaaatc ctttggtaaa
     2941 gcttctttta tacttataat cagattttta tactcttctt tccaaccttc atataaaaac
     3001 acaggtgcta ttatgaaacc aatctgataa ccagccctag caagttttac agctgactct
     3061 attcttttat ttgcagaggc tgtataatgc tcatgttcat ttataattct aggagtattg
     3121 atactaaatc gtatttctgt atgattatta tgttcagcat ccaataagct atccacatca
     3181 ctgtatttag taacaaatct aaatcttcct ttttcctctc ttccaaaaaa ctctattgtt
     3241 ttctttaaag aattagtata tggctctact ggaactgggt ctgatgtagc tgccccttca
     3301 aatatggtta cctgtggctt tctatcttca atatattttt ttgtctgttc aagtatttca
     3361 tctacattta caaacaccct tataaatggt ttatcaccta attgtgtgtt caaataacaa
     3421 tactcacatc ttcccataca tccactgaca agtggtagtt gataattagc agaaggttta
     3481 catgtttgaa acttcagact ttttctaatc ccaactacca tagtctgctt cccacttcta
     3541 tattgttcat atagattttc acctggaata tgttctttaa tcctatttga tgatatatta
     3601 attatctcta catctttatt atctctaaat ttattgtata tttttttacc cacatcagtg
     3661 tctaaagttc ctttttcgaa tattattctt ttgggattaa acatttattt cacctctact
     3721 taaagttttt aaatcgaaga attttataaa atattactta attaatcttc ccttttttaa
     3781 actcctttat ttattagaaa gtaaaataaa agcttaag
//
LOCUS       DAELLV010000286         3774 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.286, whole genome shotgun sequence.
ACCESSION   DAELLV010000286 DAELLV010000000
VERSION     DAELLV010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3774
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.286"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            15..683
                     /locus_tag="KPD58_003377"
     CDS             15..683
                     /locus_tag="KPD58_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0267104.1"
                     /translation="MENITSYFTTILCVFICLSSVFIFTQLARVFINKKKINQKIKSR
                     NGFRYDRDFIEARREEIHIKDNNNNKNKSNNKKLKEEKVFKYDNGDLYKGEFVDGKKN
                     GFGIYIFSGKEKYEGLWKDDKMHGIGKYTYRDGSIYTGEFKYGLKNGLGKLTYPNNDI
                     YKGYFLDNKKNGKGVLYKNDGNKQAGIWEDDEQCKSLDFKDLNNNKINNVYQNRLNNN
                     KLKV"
     gene            842..1348
                     /locus_tag="KPD58_003378"
     CDS             842..1348
                     /locus_tag="KPD58_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HBG0267105.1"
                     /translation="MKEYVVVDLETTGLDPYKGCEIIEIGITEIKNEQIVKNYSRLIK
                     PKGIISSFITELTHISNEMVENEEPLELVLPRFRKYIGDRTIIAHNAKFDLKFLNYYL
                     RMLDLEPINNYICTVELLKKCKSYKGKNKKLETACAYYNIENINAHRADSDTLATAKL
                     FLKIKDEY"
     gene            1476..2492
                     /locus_tag="KPD58_003379"
     CDS             1476..2492
                     /locus_tag="KPD58_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HBG0267106.1"
                     /translation="MGYYNDNDNNKIRRKKISNNKDSNKKSKHSYTSANKKNKSPNTS
                     SSKRMSEKVDSQNLIKRDRDSFTRDNVRPLNTDYNQRERRRVEYKQREKKLKSKKRKK
                     QKRIRIVRALLSIMLVALIIFSVAGAIFTVSAIKGSPEVTKKLIRSKYISSEVVSIKQ
                     MPNDLKHAIVSIEDERFYKHKGVDVISLVRSVLNNVFTDTTQGASTIDMQVSKNLLTS
                     NDKTMKRKIRDMYNAKQMNKIMTKDEILEAYLNNMYLGKNAYGAAKGAEVYFGKKVSE
                     LNLAECAMLAGMTNNPARYIDHGEAKKRQVIVLYKMHQLGYITDKEYRIAKADDTPFK
                     SEID"
     gene            2846..3550
                     /locus_tag="KPD58_003380"
     CDS             2846..3550
                     /locus_tag="KPD58_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HBG0267107.1"
                     /translation="MKKFVIEDAFWNLFPSAKIGVVICNDIDNSIKDEDYKKIILKGE
                     EEALKYLEDSEFSNNQVIKVWREAFKKFKTKKGARSSIEALLKRVHNGNNIGTINPLV
                     DIYNFISLKYALPCGGEDIDKFIGDIRLTRAIGNEEFIPLGTDENSFPYEGEIIYKDD
                     GGAICRCWNWREAVRTMLTEDTNNAFLCIELVDESRFKEFENALNELAKIVQEKLGGN
                     CKISILDIDNKEVSVD"
BASE COUNT         1618 a          324 c          657 g         1175 t
ORIGIN      
        1 aagggtgatt ttttatggaa aatataacta gttattttac aacaatacta tgtgtattta
       61 tatgcttatc atctgtattt atatttactc aactagctag agtatttatt aataagaaga
      121 agattaatca aaaaattaaa tcaagaaacg gatttaggta tgatagagat tttattgaag
      181 ccagaagaga agaaatacat ataaaggata acaataataa taaaaataag agtaacaata
      241 agaaattaaa ggaagaaaaa gtatttaaat acgataatgg tgatttatat aaaggtgagt
      301 ttgttgatgg aaagaaaaat ggatttggaa tatatatttt ttcaggtaag gaaaagtatg
      361 aaggattatg gaaagatgac aagatgcatg gaataggaaa atatacttat agagatggtt
      421 caatatatac aggtgaattt aaatacggat taaaaaatgg tttgggaaaa ttaacatatc
      481 ccaataatga catttataag ggatattttt tagataataa gaaaaatggt aagggtgtat
      541 tatataaaaa tgatggaaat aaacaagcag gaatatggga agatgatgaa caatgtaaaa
      601 gtttagattt taaagattta aataataata aaataaataa tgtttatcaa aatagattaa
      661 ataacaataa attaaaagtt taattattct aataaaaaaa tctttattta tgatggaata
      721 taggatatat ctatgttgta atttgtaaaa tcttcctata tcatataaaa ttaagtattg
      781 attagtctat tacataaata tagaatataa atatatccat ttaataataa ggagaagaga
      841 tatgaaagag tatgtagttg tggatttaga aactacagga ttagatccat ataaaggttg
      901 tgaaataata gaaataggaa taacagaaat aaaaaatgaa caaattgtaa aaaactattc
      961 aaggttgata aaacctaagg ggataatttc ttcatttata acagaattga cacatataag
     1021 taatgaaatg gtagaaaatg aagaaccact agaattagtt ttacctagat ttagaaaata
     1081 cataggtgat agaactataa ttgcacacaa tgcgaaattt gatttaaaat ttttaaatta
     1141 ctatcttaga atgttagatt tagaaccaat aaacaattat atatgtacag tagagttatt
     1201 aaaaaaatgt aaaagttata aaggaaaaaa caagaaactt gaaacagctt gtgcttatta
     1261 caacatagag aatataaatg ctcatagagc tgacagtgat acattagcaa ctgcaaaact
     1321 atttttgaaa ataaaagatg aatattaatt ataaatatag agaaaattag tgaaaatatc
     1381 ctttataaaa ttgaccctta agcttacaat tttgttcttt gatttatttt gtagaacaat
     1441 gtataatgta ccttatatga agaagggaga ctaaaatggg ttattacaat gataatgata
     1501 ataataaaat aagacgtaaa aaaattagta acaacaaaga tagtaataaa aaatctaaac
     1561 attcttatac ttcggcaaat aaaaaaaata aaagtcctaa tacaagttcc agtaaaagaa
     1621 tgagtgaaaa agtagattca cagaatctta taaaaagaga tagagatagt tttacaagag
     1681 ataatgtaag acctttaaat acagattaca atcaaagaga aagaagaaga gtcgagtata
     1741 aacaaagaga gaaaaaatta aaaagtaaaa aaagaaaaaa acaaaaaagg attagaatag
     1801 ttagggcatt attatctatt atgttagttg cactaattat attttcagtg gcaggtgcaa
     1861 tatttacagt ttcagctata aaagggtcac cagaagttac taaaaagtta attaggtcta
     1921 aatatataag tagtgaggtt gtaagtataa aacaaatgcc aaatgattta aaacatgcaa
     1981 tagtttccat agaagatgag agattttata agcataaagg agtagatgtt atttctctag
     2041 ttaggtcagt attaaacaat gtgtttacag atacaacaca aggtgcaagt actatagata
     2101 tgcaagtttc aaaaaacctg cttactagta atgataaaac tatgaagcgg aaaataaggg
     2161 atatgtataa tgctaagcag atgaataaga ttatgactaa ggatgaaatt cttgaagcat
     2221 atttaaataa tatgtatcta ggtaaaaatg catatggagc tgcaaaggga gctgaagtct
     2281 actttggtaa aaaagttagt gaattaaatt tagctgagtg tgctatgtta gctggtatga
     2341 caaataatcc tgcaaggtat attgaccatg gagaggctaa gaagagacaa gtgattgtac
     2401 tgtataagat gcatcagctt ggttatataa ctgataagga gtatagaata gcaaaagctg
     2461 atgacactcc atttaaatca gaaattgatt aaaattgtaa gaatatagta ttttcaatag
     2521 tttaaacaaa caaaaatgta ataaaaaagg tgatttgcca cccatttgcc accataatta
     2581 taatctaatg gcaaattgtt ttaatgtaca aactagataa tagaatattg tcttaaatgc
     2641 tatattatgc taaaatttaa ttgtattttg taaattaaaa aacttataat ttagttttct
     2701 agggttccgc attttataat ggtcaggtcc aagagaaaac gcgcaattgt tgtgtacacg
     2761 gaaggataaa agcctgggag atatttaaga atatttctat aggctttttt atttttacat
     2821 attttaaatt tgaggaggtt ttctaatgaa aaaatttgtt attgaggatg cattttggaa
     2881 tctatttcct agtgcaaaaa ttggagttgt tatttgtaat gatatagata actctataaa
     2941 agatgaagac tataagaaaa taattttgaa gggagaagaa gaagcattaa aatacttgga
     3001 ggactcagaa tttagcaaca accaagtgat taaagtatgg agagaagcat ttaaaaaatt
     3061 taaaacgaaa aaaggagcaa ggtcttctat tgaagcatta ttaaaaagag ttcataatgg
     3121 aaataatata ggaactatta accctttagt ggatatctac aattttatct cattaaaata
     3181 tgcattacct tgtggagggg aggatataga taaatttata ggagatatta ggctaactag
     3241 agcgattggt aatgaggagt ttatcccact agggacagat gaaaattcat ttccatatga
     3301 aggagaaata atttataaag atgatggtgg agcaatttgt agatgttgga attggcgtga
     3361 agcagtgaga acaatgctta ctgaagatac taacaatgcc tttttatgca ttgaattagt
     3421 agatgaaagt agatttaaag aatttgaaaa tgctttaaat gaattagcta aaatagttca
     3481 agaaaaattg ggtgggaatt gtaaaatttc aattttagat attgataata aagaggtatc
     3541 agtggattaa tatggaggtg ctgttgtaat ggactattct agttataaag atacttatat
     3601 ttagggtaag aaggtctatt ttagatataa cgattatcaa aggatataat atagaaaata
     3661 aaaatgtaga atataaaaag cctttaaaat aagaaaaaaa tgtattttaa aggcttttat
     3721 tatactcttt tttatttgca taattaacaa taaaaatata ttgttaatta taat
//
LOCUS       DAELLV010000287         3753 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.287, whole genome shotgun sequence.
ACCESSION   DAELLV010000287 DAELLV010000000
VERSION     DAELLV010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.287"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            250..1830
                     /locus_tag="KPD58_003381"
     CDS             250..1830
                     /locus_tag="KPD58_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HBG0267108.1"
                     /translation="MLIGTSKLSRNVDTACVEEVGIPMIVLMENAVISAMKNMDIDMY
                     NSYTVVCGVGNNGGDGLGIARHLNTYGKEVNVFLVGNLEKLSECSKINYNVLLNMGIN
                     IININKNDLSEKNYGDENEVDKIIKVEKQNIIKNKNLTIFKDRVSKSDVVVDAIFGTG
                     LKRDITGIFKEVIDIVNDNSKNTYSIDIPSGIDSDNGNVLGTCIRANKTISFEFYKRG
                     FLNYDTSALIGDVVVEKIGVPDFITRKYHDEEFITDKTFVKNNIRRRDKHGFKSDFGK
                     VSIVAGSKGFYGASFIATESAVKSGSGLVTLISSEDVQEKVSIRLTEAMTLNFEEERL
                     NKLLNSSDAIGFGPGMGDNSQTFDKLLKIVENSNCPIVLDADGLNVMKDRCYKFLEWK
                     NRFVITPHLGEMARLTGDSIGYIREHRVDVAKEFAQKYNLVVLLKGYQTVITDGKKTY
                     INPTGNSCMATGGMGDCLLGMITSFIGQGMDILEATVSGAYIHGYIGDKLSKAMYTIN
                     ATDLISNISLTMKELLDI"
     gene            2004..2450
                     /locus_tag="KPD58_003382"
     CDS             2004..2450
                     /locus_tag="KPD58_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBG0267109.1"
                     /translation="MKLIWLIVAVLFGIAEMLTPSLTLIWFSVGAVILIFLSSFIESI
                     FLQILIFAVISIAMLVVATKKIVKKDKGYKSNTNLQAMMSKKGIVTEEISPNNTGLVV
                     VEHETWTAISIDGEKIEKGSTVEVLKIEGVKLVVKKVDATVNVTNQ"
     gene            2471..3514
                     /locus_tag="KPD58_003383"
     CDS             2471..3514
                     /locus_tag="KPD58_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HBG0267110.1"
                     /translation="MGTKIVLSIVLIVVVVAISLTCIRVIKQSKVGIIMRLGKFQKVA
                     ETGVHFLIPFLDKMAYVIDLREIVIDFPPQPVITKDNVTMQIDTVVYYKVTDPVRYVF
                     EIANPIAAIENLTATTLRNIIGELDLDETLTSRDIINVKMRTILDEATDKWGIKVNRV
                     ELKNIMPPQDIQVAMEKQMRAERERREAILQAEGNKSAAILQAEGEKQSAILTAEAKK
                     EAMVRVAEGEKESAILVAEGEAEAIRQTAIAKAQGEAEMIKRTQMATAEGLKLVFSAM
                     KEADIDNNILALKSMEALEKMAEGKSTKLVLPSEAVNFLGTFKGIKEVMSDDNKEVLD
                     IKEVLNDNESLKK"
BASE COUNT         1519 a          341 c          702 g         1191 t
ORIGIN      
        1 aagattataa aaataaagag atatttagat aattagtgta aataggtttt aaatcaacat
       61 aataacttaa attatatggt ataataaatt aaataattgt tttaattcaa aattttttgt
      121 tattttaaat tatttcaaaa ctcttagttt tagtgatttt atttatgata tttatgggat
      181 taaaaagatt tcaatagata tttttggtaa gtatttaaga agtctttaac tttataggag
      241 tgataattta tgctaatagg tacatctaaa ttaagcagaa atgttgatac tgcatgtgta
      301 gaagaagtag ggattccaat gattgtattg atggaaaatg cggttatatc tgcaatgaaa
      361 aatatggata tagacatgta taatagttat acagttgtat gtggtgttgg aaataatgga
      421 ggagatggtc ttggaatagc aagacatcta aatacatatg gaaaagaagt aaacgtattt
      481 ttagtaggta atcttgaaaa actaagtgaa tgctctaaaa taaattacaa tgtactttta
      541 aatatgggaa ttaatattat aaatattaat aaaaatgatt taagtgaaaa aaattatgga
      601 gatgaaaatg aagtagataa aattattaaa gttgagaaac aaaatattat aaaaaataaa
      661 aatttaacaa tttttaaaga tagagtatct aaaagtgatg ttgtagtaga tgctatattt
      721 gggactggat taaaacgtga tataactggt atttttaaag aagttattga catagtaaac
      781 gataatagca aaaatacgta ttctatagat attccttcag gtatagatag cgataatgga
      841 aatgtattag gaacttgtat tagagcaaac aaaacgatta gctttgagtt ttataaaaga
      901 ggttttctaa actatgacac tagcgcactt ataggtgatg tagttgtaga aaagataggt
      961 gttccagatt ttattacaag aaaataccat gatgaagagt tcataactga taagactttt
     1021 gtaaaaaata atattagaag aagagataag catggtttta aaagtgattt tggaaaagta
     1081 tctatagttg caggttcaaa aggattttat ggtgcaagtt tcatagcaac tgaatctgct
     1141 gttaagagtg ggagtggact tgtaacatta attagtagtg aagatgtgca agaaaaggta
     1201 tctattagac ttacagaagc aatgacttta aattttgaag aagaaagatt aaataaatta
     1261 cttaattcta gtgatgcaat tggttttggt ccaggtatgg gagataatag tcaaacattt
     1321 gataaattac ttaagatagt agaaaattct aattgcccaa tagtattaga tgcagatgga
     1381 ttgaacgtta tgaaggacag atgttataag tttttagaat ggaaaaatag atttgtaatt
     1441 acacctcatt tgggagaaat ggccaggttg acaggagatt caattgggta tataagagaa
     1501 catagagtag atgttgcaaa agaatttgct caaaaatata atctggtggt gcttttaaaa
     1561 ggttatcaaa ctgtcataac agatggtaaa aaaacatata taaatcctac aggaaatagc
     1621 tgtatggcaa ctggtggaat gggtgattgt ttgcttggaa tgattacttc atttataggt
     1681 caaggtatgg atatattgga agctacagtt agtggagcat atatacatgg ctatatagga
     1741 gataaattga gcaaagcaat gtatacgata aatgctacgg atttaataag taacatttca
     1801 ttaacaatga aagagttgtt ggatatataa ttggtgatat ttgatactac tttgtaacaa
     1861 ttttaacaaa gtatgttgat ataatactat taattaatta atttatttta ttgttttgta
     1921 atttaaattt atattcaaaa acattgtata aacatataat ttatataggg ttttataatt
     1981 catatgaaaa gagggtagat agtatgaagt taatatggtt gatagtagca gtcttatttg
     2041 gaatagcaga aatgttaaca ccaagcttaa cattgatatg gtttagtgta ggagctgtaa
     2101 tacttatatt tttaagtagc tttatagaga gtattttctt acaaatttta atatttgcag
     2161 taatttctat agcaatgtta gtcgtagcta ctaaaaaaat agtaaaaaaa gataaaggct
     2221 ataaatctaa cactaattta caagcaatga tgtctaaaaa aggtattgtt acagaagaga
     2281 tttctccaaa taacacaggt ctagttgtag ttgaacatga aacatggacg gctatttcta
     2341 tagatggtga aaagatagaa aaaggttcta cagtagaagt attaaaaatt gaaggggtaa
     2401 aattagttgt taaaaaagta gacgccacag ttaatgtgac aaaccaatag aataaaggag
     2461 gatttatagt atgggtacta aaattgtttt aagtattgta ttaatagtag tagtcgtagc
     2521 aataagttta acttgtataa gagttataaa acaatctaaa gtaggtataa taatgagact
     2581 tggtaagttt caaaaagtag ctgaaacagg tgtacatttc ttaataccat ttttagataa
     2641 aatggcatat gtaattgacc ttagagaaat tgtaatagat tttccacctc aaccagttat
     2701 aactaaagat aatgtaacta tgcagattga tacagttgta tattataaag ttacagaccc
     2761 agttagatat gtgtttgaga tagctaatcc aatagctgct attgaaaatt taacagctac
     2821 aacactaaga aatataattg gtgaacttga tttagatgaa acattaacat caagagatat
     2881 aataaatgta aaaatgagaa ctattcttga tgaagcaaca gataaatggg gtataaaagt
     2941 aaatagagta gagttaaaaa atataatgcc tcctcaggat attcaagttg caatggaaaa
     3001 acaaatgaga gcagaaagag aaagaagaga ggcaatactt caagcagaag gtaataagtc
     3061 agctgcaata cttcaagctg aaggtgaaaa acaatctgct atattaacag cagaagccaa
     3121 aaaagaagct atggtacgtg tagcagaagg tgaaaaagaa tctgctatat tagtggcaga
     3181 aggtgaagct gaagctataa gacagacagc tattgctaag gcacaaggtg aagctgaaat
     3241 gataaaaaga actcaaatgg caacagcaga aggtttaaaa ttagtttttt cagcaatgaa
     3301 agaggctgat atagacaata atattctagc attaaaatct atggaagctc ttgaaaaaat
     3361 ggctgaaggt aagtcaacaa aactggtttt accttcagaa gcagttaatt tcctaggaac
     3421 attcaaagga ataaaggaag ttatgagtga tgataacaaa gaagtacttg atataaaaga
     3481 agttttaaat gataatgaat cattaaaaaa ataaaagagt tttgttatca taggaaggat
     3541 atggaaatta aaggtcgaat taattaaata tagcacaaaa atgcacgttg aaaagaagag
     3601 gacttataaa tttttaagtc ttcttctttg tttttattag aaagatgaaa ttataaaaat
     3661 aaattaacag aatctttata caatttatcc tttactaatg taaaaagtgt tatataatat
     3721 tatataaaac atatagcaaa taagcatggt ttt
//
LOCUS       DAELLV010000288         3730 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.288, whole genome shotgun sequence.
ACCESSION   DAELLV010000288 DAELLV010000000
VERSION     DAELLV010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.288"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(369..1091)
                     /locus_tag="KPD58_003384"
     CDS             complement(369..1091)
                     /locus_tag="KPD58_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004398740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBG0267111.1"
                     /translation="MITIKNLSKSFGDLNVLKNIDLEIAKGEIMVIVGPSGSGKSTLL
                     RCMNLLEIPTGGEIIFEGKNLVDKKTNIDEVRQNIGMVFQNFNLFPHKTILDNITLAP
                     IKLKKMTKEEAEKKAEILLSRVGLLDKKDSYPSQLSGGQKQRIAIARALAMEPDMMLF
                     DEPTSALDPEMVNEVLDVIKELAKEGMTMAIVTHEMGFAKEVADRVIFIDGGSILEDN
                     TPEEVFGNPKHERTKAFLAKVL"
     gene            complement(1150..1827)
                     /locus_tag="KPD58_003385"
     CDS             complement(1150..1827)
                     /locus_tag="KPD58_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBG0267112.1"
                     /translation="MSLDFSFLSRFGTSFLEGTGVTVSISLVALCFGFIIGIIICMAK
                     ISKSKVLRAISSIYIEVLRGTPLLVQIYIVWFGLPQLGIRFPMLFGIPSEFIASAFAL
                     SVNSGAYVAEILRSGIQSVDNGQMEASRSLGLNYWSTMRYIIIPQAIKNILPSLANEF
                     ITLVKESSIISVIGVVEIMRTADIVKNAAFRALEPLIVAAAIYFVITFTLSRLVGLLE
                     KKLSVSN"
     gene            complement(1855..2670)
                     /locus_tag="KPD58_003386"
     CDS             complement(1855..2670)
                     /locus_tag="KPD58_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBG0267113.1"
                     /translation="MKVFKKLLSLGLVLGLTLSLVGCSGGGEKTKLEQIKDNGKLVVG
                     TSAEFPPFEFHKVVDGKDSIKGFDIMLAEEFAKELGVKVEIKDMSFDGLIGALNADQV
                     DIVLAGMSPTPEREKSVDFSELYYLSRNAVIVKDADIDKVKTEDDLKKLRVGVQAGSI
                     QEEYVVNTLKMTTTKSLKAIPDLITELKNGNIDAVVTNEAVSLINVKKYDGIKMANTE
                     VGKDVTEGMAAAIKKSDNNKDFIELLNKKIKELQDGKKIEEFLNEASTEAASN"
     misc_binding    complement(2896..3106)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         1172 a          683 c          356 g         1519 t
ORIGIN      
        1 aaaagttaaa tttataagct tacttgttat aaaaataact tttataattt atttcaaaaa
       61 aaatattgat ttttgtgtcg cattttgtta taataaatta aggttatccc cctggtaacc
      121 ctagttagtt aatatatcta ttcccaatac cctttcataa ggatatattt tatatttatt
      181 aaaaatcgga ttagtaacgg aaattaattc ggtggaaaaa aagagcttac cccgagctct
      241 tttttttctg caaaaaatcg taaaaataaa gttaatatta atataacttt aattttaaaa
      301 ttaatattaa ccatcactaa aaaaggatgt attgaatttc taatatcaat aacatccttt
      361 ttatataatt atagcacttt tgctaaaaat gcttttgttc tttcatgttt tggatttccg
      421 aaaacttctt ctggagtatt atcttcaagt atacttcctc catctataaa aataactcta
      481 tctgcaactt ccttagcaaa tcccatctca tgagttacaa ttgccattgt cataccttct
      541 ttagcaagtt cttttataac atccaaaact tcgtttacca tttctgggtc aagagcagat
      601 gttggctcat caaacaacat catatctggt tccatagcaa gtgctctagc tattgctatt
      661 ctttgctttt gtcctccaga taattgtgat ggatatgaat cttttttatc taaaagacca
      721 actctactca acaaaatttc tgcttttttt tctgcttctt ccttagtcat cttttttaat
      781 ttaattggtg ctaatgttat attatctaaa atagtcttat gtgggaataa attaaagttc
      841 tgaaacacca tacctatatt ttgtctaacc tcatcaatat tagttttttt atcgaccaaa
      901 ttttttcctt caaatattat ttctccacct gtaggtattt ctagtaaatt catacatcta
      961 agaagtgtac ttttaccaga accacttgga cctactataa ccattatttc gcctttagcg
     1021 atttctaagt ctatattttt taatacattt aaatctccaa aagatttact taaatttttt
     1081 attgtaatca tattatcact ttccttttta ctattctatg ttttccctaa atgctaaaca
     1141 tgctctcatc tagttagaca cagatagttt cttttctaat aatccaacta atcttgatag
     1201 agtaaatgtt ataacaaagt aaattgctgc tgctactatt aatggctcta atgctctaaa
     1261 tgctgcattt tttactatat cagcagtacg cattatctca acaactccta ttacagatat
     1321 tattgaagat tcttttacaa gtgtaataaa ctcatttgct aatgaaggta atatattttt
     1381 tatagcttga ggtattatta tatatctcat agttgaccaa taatttaatc ctaatgatct
     1441 acttgcttcc atttgtccat tatcaactga ttgtatacct gatctaagta tctctgccac
     1501 gtatgcacca gagtttactg ataatgcaaa tgcactagct ataaattcac ttggtatacc
     1561 aaacaacata gggaatctta ttcctaattg aggtaaacca aaccatacta tatatatctg
     1621 tactaataat ggtgtccctc ttaaaacctc aatatagatt gatgatattg ctcttaatac
     1681 tttgctcttt gatatttttg ccatacagat aattatacct attataaatc caaagcataa
     1741 tgccacaaga gaaattgata ctgtcacacc tgttccttcc aaaaaagaag tcccaaatct
     1801 acttaaaaaa ctaaaatcta aactcataat ttcatctccc acataacttt cttactaatt
     1861 tgaagcagct tcagtagatg cttcatttaa aaattcttct atcttcttgc catcttgtaa
     1921 ttcttttatc tttttgttta ataattctat aaaatctttg ttattgtcag attttttgat
     1981 tgctgctgcc ataccttctg ttacatcttt cccaacttca gtatttgcca tttttattcc
     2041 atcatatttt ttaacattta ttaaagaaac tgcttcatta gtaacaactg catctatgtt
     2101 tccatttttt aattctgtaa ttaaatcagg tattgctttc aaagattttg tagttgtcat
     2161 ttttaaagtg ttaactacat attcttcttg gatacttcct gcttgaactc caactctaag
     2221 tttctttaaa tcatcttcag tttttacttt gtctatatct gcatctttaa ctataactgc
     2281 atttctactt aaataatata attctgaaaa atcaacactc ttttctctct ctggtgttgg
     2341 agacatacct gcaagaacaa tatcaacttg gtctgcattt aaagcaccta ttaatccatc
     2401 aaatgacata tccttaattt caacctttac accaagctct tttgcaaatt cttcagccaa
     2461 cattatatcg aatcctttta ttgaatcttt tccatctact accttatgaa attcaaatgg
     2521 aggaaattct gcactagtac caacaactaa ttttccattg tcttttattt gttctaactt
     2581 tgttttttct cctccaccac tacatcctac tagcgataat gtcaatccta atactaatcc
     2641 taaacttaat aattttttaa atactttcat tttaactacc cctctctttt aattataatt
     2701 tccctcagat tttaatactt aaatgtttcg agttacaaaa aaagtctctc agtctttaat
     2761 gactaagaga cgactttacc gcgttaccac tcttattgat aaatacttat attactttca
     2821 aaatttgtga ctgttagcat ttaagctgta tggatatttt tatttttaaa tcaaaaaagt
     2881 cccttagctt tacagctaag agacgaatta tatccgcgtt accactcttt ttgataagta
     2941 agtacttatc ctctcaatcc cttaaggcgg gtaaaccaat tatcatacta aagtttctga
     3001 taatctcctc cagagctctc ttcacataga cttcattgct aggcttacac caacccctaa
     3061 ctcgctgtaa atccatatat gctactttct ctatcattgg atttgtttcc ttaaattgtt
     3121 aatacaattg tacttcctta taatattttt gtcaataagt tttttatttt tttaataatt
     3181 tttacttgta aattatctaa atactactgt ttatatttat tgatattagt tttgtaatta
     3241 taaatagtat atttcatatt tttagctatt tcttaaataa tactaaataa tatctttcac
     3301 attgttgctt tttcttaaat tacactaaat aatatctttc acattgttgc tttttcttaa
     3361 acaatactaa ataatatctt tcacattgtt gcttttctta aattacacta aataatatct
     3421 ttcacactgt tgctttttct ttaataatac taaatagtat ctttcacact gttgcccttt
     3481 ctttaataat actaaataat atctttcaca ttgttgcttt ttcttaaatt acactaaata
     3541 atatctttca cactgttgct ttttctttaa taatactaaa tagtatcttt cacactgttt
     3601 ctctttcttt aataatacta aataatattt tccacattgt tgctttttct aaacaactat
     3661 aaataatata ttaaaccctt atttcttttt ctaataagtt aaataataca ttttacacat
     3721 atctatttgt
//
LOCUS       DAELLV010000289         3717 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.289, whole genome shotgun sequence.
ACCESSION   DAELLV010000289 DAELLV010000000
VERSION     DAELLV010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.289"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            203..1075
                     /locus_tag="KPD58_003387"
     CDS             203..1075
                     /locus_tag="KPD58_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0267114.1"
                     /translation="MEMKIAVSNLSKRFGKKQALDNINLEIDSGMFGLLGRNGAGKTT
                     FMRILATLLSKTEGSIHMCGVPIENTKEIRNMVGYLPQDFSMYPNMTVYKAMDYLAVL
                     SDIPLRKRRKIIAKLLSKVNLTNYQNVKVKSLSGGMNRRLGIAQALIHNPRVLIVDEP
                     TAGLDPEERIRFRNLLCEIANDRIVILSTHIVGDIEATCENVAILNSGKVIYNGSTEA
                     LAKLAEGKVYSIEVDKKDIENIKSRFIVIGMLTHGGKAILRIISDDKPFETAVNCNPT
                     IEDGYMLIMGGDNI"
     gene            1072..2361
                     /locus_tag="KPD58_003388"
     CDS             1072..2361
                     /locus_tag="KPD58_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0267115.1"
                     /translation="MNLFIKECKNILKSMIYYIFIVVLVFFYISQLGSSVSNDVSKNI
                     PNDGNPLVAPQKVENIEEYYSVHNQYPYGQKESMKTEKVIPNATINLIREYQKNTYTT
                     YPTGVVKYIKLDQNKTKTIEKYIEEITGKSISEINSLWKTDESIKIDYSKVSLERFEE
                     IMQRVDDILGGGSAYAFDKLQRFGVVPVTYDEAMKNYNYTIQKDKVSNGYARIFCDYI
                     GIVLSIFPVFVAVFMSLKDKRSRMREIIYSKQVSSCKIVLSRYFALVFMMILPVLILG
                     MKDLLPLIFFAKDKGISIDNLAFIKYTLWWILPTLMIVTAVGMSITILTDTPVAIIVQ
                     LIWWFYSINSTGLKGDYSTMGLMIRHNSVLNGDIIIQNFNQIMINRIVISLEALVLVL
                     LAIFLYEQKRRGNLDVWAKFSKLFSSNKRKLQIKHFN"
     gene            2291..2935
                     /locus_tag="KPD58_003389"
     CDS             2291..2935
                     /locus_tag="KPD58_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0267116.1"
                     /translation="MFGQSFQNYLVLIKENFKLSTLTNLICSIILLCMIPLIQGVANL
                     DRISTAICLENYVAIIGIVMLVPVFSLEQSKEIDEIVSSKALSQVIVQLIRILMSIIG
                     IVVLIIGFVNVLIYLGCNFPVVEYIIGSFASAMFIGSLGMFFSNLFNNTIIGYMISIG
                     YLILNMMTKGKYVGKFFIMSMSQGSFDEKYWLILGSVVLIIASLLIKPVKRKIR"
     gene            complement(3202..3666)
                     /locus_tag="KPD58_003390"
     CDS             complement(3202..3666)
                     /locus_tag="KPD58_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0267117.1"
                     /translation="MNLNKVKFTEEHAIEVTNWKYEGKYSIYNLPPWEEIKKKNFSLA
                     KEDKRKNFISFINDNKELIGFINLLDEGSSVFFGIGMKPNYCGMGIGKEIIGLGLQEC
                     RNKYSTKPIVLNVRTWNMRAVKCYESQGFKIVETKVQKTHLGDGEFFVMRYQ"
BASE COUNT         1424 a          427 c          568 g         1298 t
ORIGIN      
        1 tattaaatta taacttcata aacttaaaaa atttcagaaa caaagatact ttaatttaat
       61 taaaaacaga aaaattaaaa tctttagaaa aaatttagaa atttataaat aatatcttaa
      121 gttttagaat ttattatgaa aatacagtaa aaatttataa aaataaaatt ctataaaatt
      181 gtttctgggg gtgtatttat atatggaaat gaaaatagca gtatccaatc ttagcaaaag
      241 atttgggaaa aaacaagcac ttgataatat aaatcttgaa atcgacagtg gtatgtttgg
      301 tcttttagga agaaatggag caggaaaaac cacatttatg agaattttag ccactttatt
      361 atcaaaaaca gaaggttcaa tacatatgtg tggagttcct attgagaata ctaaggaaat
      421 tagaaatatg gttggttatt tgccacaaga tttttcaatg tatcctaaca tgacagtata
      481 taaagcaatg gattatttag cagttttatc ggatatacca ctaagaaaaa gaagaaaaat
      541 tatagcaaag ttattaagca aagtaaacct tactaattat caaaatgtga aagtaaaatc
      601 tttatctgga ggtatgaata gaaggcttgg gatagcacaa gcattaattc ataatcctag
      661 agttttaata gtagatgagc cgacagcagg tcttgaccca gaagaacgta taagatttag
      721 aaatttacta tgcgaaattg caaatgatag aatcgttatt ttatctacac atattgttgg
      781 agatatagaa gctacatgtg aaaacgtagc tatattgaat agtggaaaag tcatatacaa
      841 tggctcaaca gaagcattag caaaactagc tgaagggaaa gtatatagta ttgaagtaga
      901 taaaaaagat attgaaaata taaaaagtag atttatagtt ataggaatgt tgactcatgg
      961 tggaaaggct atacttagga ttatatcaga tgataaacct tttgagacag cagtaaattg
     1021 taatccaact attgaagatg gctatatgct tataatggga ggagataata tatgaatcta
     1081 tttataaaag aatgtaaaaa tatattaaag agtatgatat attatatttt tattgtagtt
     1141 ttagtttttt tctatatttc tcaacttgga agttcagttt caaatgatgt aagtaaaaat
     1201 attcctaatg atggaaatcc attagttgct ccacaaaaag tagagaatat tgaggaatat
     1261 tactctgtac acaatcaata tccatatggt caaaaagaat ctatgaaaac agaaaaagtc
     1321 attccaaatg caactataaa cctaattaga gaatatcaaa aaaacacata tacaacgtat
     1381 ccaacagggg tagttaagta tataaaatta gaccaaaata aaacgaagac catagaaaaa
     1441 tatatagaag aaataactgg taaaagtatt agcgaaataa attcactttg gaaaactgat
     1501 gaaagtataa aaatagatta ttctaaagta tctcttgaaa gatttgaaga aataatgcaa
     1561 agagtagacg acattttagg aggagggtct gcatatgctt ttgacaagtt acaaagattt
     1621 ggagtagttc cagttaccta tgatgaagct atgaagaatt acaactatac aatacaaaaa
     1681 gataaagtta gtaatgggta tgcaagaatt ttttgcgatt atataggtat agttttaagt
     1741 atatttccag tatttgtggc agtttttatg tcacttaaag ataagcgttc aagaatgaga
     1801 gagattattt attcaaaaca agtatcttca tgcaaaatag ttttatcaag atattttgca
     1861 cttgtcttta tgatgatatt gcctgtatta attttaggta tgaaagattt acttcctttg
     1921 attttctttg ctaaagataa aggtatatct attgataact tagcatttat aaaatataca
     1981 ttatggtgga tactaccaac tcttatgata gttacagctg taggaatgag tattactata
     2041 ttaacagata caccagtagc aattattgtg caattaattt ggtggtttta cagtataaat
     2101 tctacaggac tcaaaggtga ttattcaaca atgggactaa tgataagaca taatagtgta
     2161 ttaaatggag atattattat acaaaacttc aatcaaatta tgattaatag aattgtgata
     2221 agcttggaag cattggtgct tgttttgtta gctatattct tatatgagca aaaaagaagg
     2281 gggaatcttg atgtttgggc aaagttttca aaactattta gttctaataa aagaaaactt
     2341 caaattaagc actttaacta atttaatatg ttcaattata ttgctatgta tgattcctct
     2401 aatacaagga gtagctaatt tagacagaat ttcaacagct atatgtttag aaaattatgt
     2461 tgcaataata gggatagtaa tgttggtacc tgtattttcg ctagagcaat caaaagaaat
     2521 tgatgagata gtatcttcaa aagcattatc tcaagttatt gtacaattaa ttaggatact
     2581 gatgtcaatt ataggtatag tggtattaat cataggtttt gtaaatgttt taatatatct
     2641 tggatgtaat tttccagtag ttgagtatat tattggaagt ttcgcatcag ctatgtttat
     2701 aggaagttta ggaatgtttt tctcaaattt atttaataat acaatcatag gttatatgat
     2761 atctatagga tatttaattt taaatatgat gactaaaggc aagtatgttg gaaaattttt
     2821 tataatgtct atgagtcaag gtagttttga tgagaaatat tggctgatac ttggaagtgt
     2881 tgtgttgata attgcatctc tattaataaa accagtaaaa aggaagatta gataataaaa
     2941 tgagagctta gataaaataa aggtaataca aataaaaagt acatttatga aatataaatc
     3001 tatttttata tacacaaacc tacttacaat ctctttatat atgtagatag atttatatat
     3061 ccgtagtata aaatatatat gtataccaaa ttttatcaaa agctctcaaa tttatatttc
     3121 aaagacactt attgctatgg cctacacata tgtatttttt cattttaatt gttgtattaa
     3181 caataagact attgttataa actattgata cctcataaca aaaaattctc catctcctaa
     3241 atgcgttttc tgtaccttag tctcaactat tttaaatcct tgagactcat aacattttac
     3301 agctctcatg ttccaggttc ttacatttaa tacaattggt ttagtactat atttatttct
     3361 acattcttgt aatcctaggc caattatttc tttaccaatt cccattccac aataattcgg
     3421 tttcatacca ataccaaaga atacagagct tccttcatct aataagttta taaaacctat
     3481 caattcttta ttgtcattta taaaacttat aaaatttttt cttttatctt cttttgctag
     3541 tgaaaaattc ttctttttaa tttcctccca tggaggtaga ttgtatattg aatatttacc
     3601 ttcatatttc caattagtaa cttcaatagc atgttcttca gtaaatttaa ctttatttaa
     3661 attcatatgt cctcctatta aaatatttaa ggtaattaat ggtattttaa agtttta
//
LOCUS       DAELLV010000290         3701 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.290, whole genome shotgun sequence.
ACCESSION   DAELLV010000290 DAELLV010000000
VERSION     DAELLV010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.290"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(111..476)
                     /gene="rplL"
                     /locus_tag="KPD58_003391"
     CDS             complement(111..476)
                     /gene="rplL"
                     /locus_tag="KPD58_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBG0267118.1"
                     /translation="MTIEQILEAIENMKVLELNELVKAAEEKFGVSASAPVMVAGAAA
                     GGPAAEEKTEFDVVLTDVGSSKVGVIKAVREITGLGLKEAKEVVDNAPKTVKEGASKE
                     EADQIKEKLEAAGAKVEVK"
     gene            complement(534..1040)
                     /locus_tag="KPD58_003392"
     CDS             complement(534..1040)
                     /locus_tag="KPD58_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBG0267119.1"
                     /translation="MRKAIEIKSEVVSEIVEKLQKSSAAVVVDYKGLTVEEVTELRKQ
                     MREAGVDYKVYKNTLVRRAAKEVGIEQFNDELLVGTNAIAFGYDDPVAPARILKGFMD
                     SHPKMKLKMGIVEGAFYDESKIVEMANIPSREVLIAKLLGSLKAPVSNFAYLIDAIAK
                     KAEGQEEA"
     misc_feature    complement(1083..1211)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(1263..1961)
                     /gene="rplA"
                     /locus_tag="KPD58_003393"
     CDS             complement(1263..1961)
                     /gene="rplA"
                     /locus_tag="KPD58_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBG0267120.1"
                     /translation="MAKKGKRYAGALQKVDRTKFYDASEALTLVSDIAGAKFDETVEA
                     HIKLGVDSRHADQQVRGAVVLPHGTGKTKRVLVFAKGEKAKEAEQAGADFVGAEELVQ
                     KIQGENWFDFDIVVATPDMMGVVGRLGRVLGPKGLMPNPKSGTVTFDVAKAIDEIKAG
                     KVEYRLDKTNIIHVPVGKVSFGGEKLTENFTALMDAIIKAKPAAAKGQYLRSITVASS
                     MGPGVKINPARTAE"
     gene            complement(2032..2457)
                     /gene="rplK"
                     /locus_tag="KPD58_003394"
     CDS             complement(2032..2457)
                     /gene="rplK"
                     /locus_tag="KPD58_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBG0267121.1"
                     /translation="MAKKVIGQIKLQIPAGKATPAPPVGPALGQHGVNIMGFTKEFNA
                     KTADQAGMIIPVVITVYQDRSFSFITKTPPAAVLIKKALNLKSGSGEPNKKKVAKMTS
                     AQVREIAELKMPDLNAASVEAAMSMIAGTARSMGVVIED"
     gene            complement(2496..3038)
                     /gene="nusG"
                     /locus_tag="KPD58_003395"
     CDS             complement(2496..3038)
                     /gene="nusG"
                     /locus_tag="KPD58_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination factor
                     NusG"
                     /protein_id="HBG0267122.1"
                     /translation="MSELQEASWYVVHTYSGHENKVKATIEKAVKTRGMEDCIRQVVV
                     PTEEVVETTKTGKEKTRQRKVYPSYVLVKMIITDESWYVVRNTKGVTGFVGPGSKPVP
                     LSEDEVKAMGIDTTDPKVVNSDIDFEIGDTVKVSQGPFSGQIGNIEEIDLENREVKVC
                     INAFGKRTLFVIELEGIEKI"
     gene            complement(3058..3279)
                     /gene="secE"
                     /locus_tag="KPD58_003396"
     CDS             complement(3058..3279)
                     /gene="secE"
                     /locus_tag="KPD58_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBG0267123.1"
                     /translation="MAAQKNDGAKTKKRFSLFGYLKETKQELKRVTWPTKKELFKNTS
                     IVLTVVISCTILVWGIDTILSGALALLLK"
     gene            complement(3305..3454)
                     /gene="rpmG"
                     /locus_tag="KPD58_003397"
     CDS             complement(3305..3454)
                     /gene="rpmG"
                     /locus_tag="KPD58_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004402360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBG0267124.1"
                     /translation="MRVKVTLACTECKQRNYNTTKNKKNNPDRIELQKYCRFCKKHTT
                     HKETK"
BASE COUNT         1019 a          739 c          448 g         1495 t
ORIGIN      
        1 atataaattt tttttatttt ataaaaatac tttctttata attccaaata ctttctttat
       61 agttttttat aaataaaaaa agatacccaa tgggtatctt tttaaagaaa ctacttaact
      121 tctactttag ctccagcagc ttctaatttc tcttttattt gatcagcttc ttctttagaa
      181 gctccttctt taactgtctt aggagcattg tcaactactt ctttagcttc ttttaatcct
      241 aatcctgtta tttctctaac tgcttttata actccaactt ttgaagaacc aacgtcagtt
      301 aatactacat caaactcagt tttttcttca gcagctggtc caccagcagc tgcaccagct
      361 accattactg gagctgaagc tgatacaccg aatttttctt cagcagcttt aactaattca
      421 tttaattcta aaactttcat attttctata gcttctaata tttgttctat tgtcattttt
      481 agcacctccg aattttattt caaattaata tttataattt tttagttaag caattaagct
      541 tcttcttgac cttctgcctt tttcgctatt gcatctatta agtatgcgaa gtttgatact
      601 ggagctttta agctaccaag taatttagcg ataagaactt ctcttgatgg tatgttagcc
      661 atttcaacaa tcttagattc atcatagaat gcaccttcca caatacccat ctttaatttc
      721 atttttggat gagaatccat gaatcctttt aatattctag ctggagcaac tggatcatca
      781 tatccaaatg ctatggcatt agttccaact aataattcat cattaaattg ctctatacca
      841 acttctttag ctgctcttct aactaaagta tttttgtata ctttgtaatc tacaccagct
      901 tctctcattt gttttcttaa ctcagttact tcttcaactg ttaatccttt gtaatctaca
      961 acaacagcag cagaagattt ttgtaacttt tcaactatct cagaaacaac ttctgattta
     1021 atttctatag cttttctcat atagatgtgc acctccttca ccaaataggc ttagtattta
     1081 ccgcaacatt ataaaaagct tttgttgcca aaagtagaca tcaaaagctt tagtaatctc
     1141 aatttatcta aaacctcggc gggatattaa gctctcgcac ccactatcta cggcaaatct
     1201 attctttttt acattgacaa gtgatataat aacatttttg tcaacctgtg tcaacagttt
     1261 tattattcag ctgttcttgc tgggtttatt ttaactccag gtcccattga agaagctact
     1321 gttatacttc ttaaatattg tcctttagca gcagctggtt tagcttttat tatagcatcc
     1381 attaatgcag taaagttttc agttaatttt tctccaccaa atgatacttt tcctactgga
     1441 acgtgtatta tgttagtttt gtctaatctg tattcaactt taccagcttt tatttcatct
     1501 atagctttag ctacatcaaa tgtaactgtt cctgatttag ggtttggcat taaaccttta
     1561 ggtcctaata ctctacctaa tctacctact acacccatca tatctggagt agcaactact
     1621 atatcaaagt caaaccagtt ttcaccttgt attttttgaa ctaattcttc agctcctaca
     1681 aagtcagcac cagcttgttc agcttcttta gctttttcac ctttagcaaa aactaaaact
     1741 ctttttgttt taccagttcc atgaggtaat acaactgcac ctcttacttg ttggtcagca
     1801 tgtcttgagt caacacctaa tttaatgtgt gcttctactg tctcatcaaa tttagctcca
     1861 gctatatctg aaactaatgt taatgcttct gatgcatcat aaaatttagt tctatctact
     1921 ttttgtaatg cacctgcata tcttttacct tttttagcca ttttattttc ctcctcgtgg
     1981 ttaatttcgg cttctagcct cccactttat aaaaaaaagt gtatctttta attagtcttc
     2041 gattacaaca cccatgcttc tagcagtacc agctatcata ctcatagcag cttctactga
     2101 tgcagcattt aagtcaggca tctttaattc agctatttct cttacttgag ctgaagtcat
     2161 tttagcaact tttttcttgt ttggttctcc agaacctgat tttaagttta atgctttttt
     2221 aattaaaact gcagctggtg gagtttttgt tataaaactg aatgatctat cttgatatac
     2281 agttataaca actggtatta tcattccagc ttgatctgca gttttagcat taaattcttt
     2341 tgtgaatccc attatattaa caccatgttg tcctaatgct ggtccaactg gtggcgctgg
     2401 agtagccttt cctgcaggta tttgtaattt tatttgacct ataacttttt tagccatttt
     2461 gagcgcacct cctcagattt ttctataata tagtattaga ttttttctat accttcaagc
     2521 tctattacaa atagagtcct tttaccaaat gcatttatgc atactttgac ttctctattt
     2581 tctaagtcaa tttcttctat gttacctatt tgtccactaa atggtccttg agatactttt
     2641 actgtatctc ctatttcaaa atcaatatcg ctatttacta ctttagggtc tgttgtgtct
     2701 atccccatag ctttgacttc gtcttcactt aatggcactg gttttgaacc aggtccaacg
     2761 aaacctgtaa ctcccttagt atttcttact acataccaag attcatcagt tattatcatt
     2821 ttaaccaaaa cataacttgg ataaacttta cgttgtctag ttttttcttt tccagtttta
     2881 gtagtttcca ctacttcttc tgttggaaca actacttgtc ttatgcagtc ttccatacct
     2941 cttgttttaa ctgctttctc aattgtagct ttaactttat tttcatgtcc cgaataggta
     3001 tgaactacat accaacttgc ttcttgtaat tctgacattt aacttacctc ttttcgtcta
     3061 ttttagtaat aacgctagtg caccagataa tatagtgtct ataccccata ctaatatagt
     3121 gcatgaaata actacagtta aaactatact tgtgttttta aacagttcct tttttgtcgg
     3181 ccatgtaact ctttttaatt cttgtttagt ttcttttaag tatccaaaca aactaaacct
     3241 ttttttagtt tttgcaccgt catttttttg ggcagccatt ccatactcac atccttataa
     3301 aaaactattt tgtttcttta tgagttgtat gctttttaca gaatctacaa tatttttgta
     3361 attctattct gtctggattg tttttcttat tcttagtagt gttgtagttt ctttgcttac
     3421 actctgtaca tgctaaagtt actttaactc tcatctgata cacctccaac tttgtaatac
     3481 aataatatat attgttaata tatgtgaata taatatcttt aaaatatgta tttattaaaa
     3541 atctacagta ttcaagatat tattaacttc ctataatcat taccttgtat aagttatcac
     3601 aaaatcctat gctgtgtcaa tgtttttgta tttctatgtt aaaaaaagag gagcataacc
     3661 cctctttttg ttattccttt taatccatca gtttagtaaa g
//
LOCUS       DAELLV010000291         3697 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.291, whole genome shotgun sequence.
ACCESSION   DAELLV010000291 DAELLV010000000
VERSION     DAELLV010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.291"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            175..2199
                     /locus_tag="KPD58_003398"
     CDS             175..2199
                     /locus_tag="KPD58_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0267125.1"
                     /translation="MDKKKLILITHDKLNSDHYKEELTNFFGDEIIIETQNILDGIKE
                     NLEGDVVLSLSPLTSNFLIKHFKEDIEIIHGTKALSKLGYEKMMKLPPGTKSLLMTTN
                     KTSAFEMATYLYKIGINHIDFVPTYPDCDEIYDLDTAITPGQIRFIPKYIKNIVDLGW
                     RKISLDTYMSLLVVLKLKNEKFIEKLYKLSKETLSHDFLNTSLDNISKLKTILYMTID
                     EIGDGLIFFNTFNKVTFVNKSLLNMLGLDEKLIKSPSLMEYMPNSFLDKITKNLNIDN
                     MIIYIDEIDKKFILSKKPFYLYKNIEGCLITLKDVNNIEILEQKIRSDSVKRGYVAKY
                     KFNNIIGNSSIIKDCIKKAKKMALTDNPILITGETGTGKEAFTQSIHNHSNRKNKPFV
                     AINCASLPSELLESELFGYEDGSFTGAKKGGKKGLFELAHTGTIFLDEIGDMPHDLQV
                     KLLRVLQEKEIRKIGGTSIIPIDVRILAATNKDLEKLIIENKFRMDLFYRISMFTLDL
                     PPLRKRLEDIPLLLESFLKELPYKNIKLDKSLLEALNSYTWMGNIRELRNCIEYMAYM
                     GSNYLTINDLPQNISSKLNNNHMSSNMSIFNDLNQYDKNICISILKSLHMKPMGRTKL
                     MKFMEYNVTEYEVRNMLEYLTRNGYLISSKGRKGSSITEKGIKIIENNII"
     gene            complement(2429..3616)
                     /locus_tag="KPD58_003399"
     CDS             complement(2429..3616)
                     /locus_tag="KPD58_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HBG0267126.1"
                     /translation="MLRKITAFMLSICLIIGLTGCSLLVGKKDKEKIIDDFKVAVVTQ
                     PLSENKVQYNMVEEMAKEYEEENKIDKDKDGQTKVKQTIKHVVLPENFTSNIDSAINK
                     IVKLADDKEVQAIVVSTDQAGLLPALQKVKEKRPEIITISAPMGDDKNQLSQFVDVNL
                     GVSAEERGKVLAERSKEMGAKAFIHYASTDDLKDVNIAKRLEMIKETCKNIGLPFVQV
                     NTPNINTEEDKNKVKQFLNEDIEKQVKKYGKDINVFGVNEYMDEVILTKALELKYIVA
                     EQSNPSPIQTYPSVMGLKISEKDAQNYDKINDMISEKAKAFGMSNRLGGYPMPMDAFL
                     PSVAIYLATEMVKQDLTQEDVCDPDYLEAFTELRFGIGSEFTPLTEVLYNYQSVILSQ
                     LIY"
BASE COUNT         1410 a          518 c          465 g         1304 t
ORIGIN      
        1 attttcctta tcttgcaaaa ataattttaa aataggtttt tataggataa aatcctaaaa
       61 aaacctatta tttaatcaaa tatataaatt tatactacta tttgttgtat aatataactt
      121 gccttatact ttaaatactt taaaaataaa tactattttg ttaggagaaa taaaatggat
      181 aaaaagaagt taatccttat aacacatgat aaattgaata gtgaccacta caaagaagaa
      241 ttaacaaact tttttggaga cgaaataatc atagaaacac aaaatatact tgatggaata
      301 aaagaaaatt tagagggaga tgtagtgctt tctctatcac ctttaactag taatttttta
      361 ataaaacatt ttaaggaaga tattgaaatt attcatggaa cgaaagcact ttcaaaactt
      421 ggatatgaaa aaatgatgaa actacctcca ggtacaaaat cgttacttat gacaaccaat
      481 aaaacttcag catttgagat ggctacttat ctatataaaa ttggtataaa tcatatagat
      541 tttgtaccga catatccaga ttgtgatgaa atatatgatt tagatacagc tataacacca
      601 ggtcagatta gatttatacc aaaatacata aaaaatattg ttgatttagg ttggagaaaa
      661 atctcattag atacatatat gtcattatta gtagtattaa aactaaaaaa tgagaaattt
      721 atagaaaaac tatacaaatt gtccaaagag acactaagtc atgatttctt aaatacatca
      781 ttagataata tatctaaatt aaaaactata ctttacatga ccattgatga aataggtgat
      841 ggtctaatat ttttcaacac ctttaacaag gttacttttg taaataagtc tttacttaat
      901 atgttgggac tagatgaaaa acttataaaa tcaccttcct taatggaata tatgccaaat
      961 agtttcttag ataaaattac aaagaactta aatatcgata atatgattat atacatagac
     1021 gaaatagata aaaaattcat actttctaaa aaaccatttt atctttataa aaatatagag
     1081 ggttgcttaa ttacattgaa agatgtaaac aatatagaaa tattagaaca aaaaataaga
     1141 agtgattctg tcaaaagagg ttatgtagct aaatataaat ttaataatat tattggaaat
     1201 agttctatta taaaagattg tattaaaaag gcaaaaaaaa tggctcttac agataatcct
     1261 atattaataa caggtgaaac aggaacaggt aaagaagcct ttactcagtc aatacacaat
     1321 cattcaaata gaaaaaataa accttttgta gcaataaatt gtgcttcatt accaagtgaa
     1381 ttacttgaaa gtgaactatt tggatatgaa gatggttctt ttactggagc aaaaaaaggt
     1441 ggaaaaaaag gattattcga attagctcac actggcacta tatttttaga tgagatagga
     1501 gatatgccac atgatttaca ggttaaactt ttaagagtct tacaagaaaa agaaattaga
     1561 aaaattggtg gtacaagtat tattccaatt gatgtgagaa tattagctgc cacaaataaa
     1621 gatttagaga aattaattat agaaaataaa tttaggatgg atttatttta taggataagt
     1681 atgttcactt tagatttacc tcctcttaga aagagattag aagatatacc tcttctttta
     1741 gaatcatttt taaaagaact tccctataaa aacataaaac tcgataagag tcttttagaa
     1801 gctttaaatt catacacttg gatggggaat attagagaac tgagaaattg tattgaatat
     1861 atggcttaca tgggttccaa ttatcttaca ataaatgatt tacctcaaaa catatcttca
     1921 aaattaaata ataatcatat gtcaagtaat atgtccatat tcaatgattt aaaccaatat
     1981 gacaaaaaca tatgcatttc tatcctaaaa tcattgcaca tgaaaccaat ggggaggaca
     2041 aagttaatga aatttatgga atataatgtc acagaatacg aagtaagaaa tatgctagaa
     2101 tatttgacac gcaatggata cttaatttct agtaaaggaa gaaaaggctc atcaataact
     2161 gagaaaggca taaaaataat agaaaataat ataatttaaa cctatagtaa ttcatcttta
     2221 ctttatcaaa atttatatat aattataatt ttttattttt ctataaattt tttataaaat
     2281 aataaaactt atttatatta aaaacgaagc tatctcaata tagggataag cttcttttta
     2341 attgttaaaa agataaaaat atatttgatt taatagaaaa agagtgcctc ataaactaaa
     2401 aagttcattt tgagacactt ccttaatatt aataaattaa ttggcttaaa attactgatt
     2461 gataattata aagtacctct gttaaaggag taaattctga accgattcca aatcttaatt
     2521 ctgtaaacgc ctctaaataa tctgggtcac atacatcttc ttgtgttaaa tcttgtttca
     2581 ccatttcagt agcaagataa attgctactg atggtagaaa agcatccatt ggcataggat
     2641 atccaccaag tctatttgac atgccaaaag ccttagcctt ttcacttatc atgtcgttta
     2701 ttttatcata attttgagca tctttttcag atatttttag acccataaca ctaggatatg
     2761 tttgtattgg tgatggattg ctttgctctg caactatata ctttaattct aaagccttag
     2821 tcaatataac ttcatccata tactcattta caccaaatac atttatatct tttccatatt
     2881 ttttaacttg tttttcaata tcttcattta aaaactgctt aactttgttt ttgtcttctt
     2941 cagtatttat atttggagta ttaacttgta caaatggcaa tcctatattt ttgcaagtct
     3001 ctttaatcat ttctaatctt ttggctatat taacatcttt caaatcatct gtagaggcat
     3061 aatgaataaa agcttttgct cccatttctt ttgaacgttc agctaatact tttcctcttt
     3121 cctctgcact aacccctaga ttcacatcaa caaattggct taattggttt ttatcatctc
     3181 ccataggggc agaaatagtt attatttctg gacgcttttc ctttaccttt tgtaaagcag
     3241 gtaaaagccc tgcttggtca gttgaaacta caatagcctg aacttcttta tcgtcagcca
     3301 actttactat cttattaata gcactgtcta tattagaagt aaaattctct ggcaatacaa
     3361 catgctttat agtttgttta actttagttt gaccatcttt gtctttatca attttattct
     3421 cttcctcata ttcttttgcc atctcttcca ccatattgta ttgtacttta ttttcagata
     3481 atggttgagt taccactgca actttaaaat catctataat tttctcttta tctttcttgc
     3541 ctacaagtaa actacaacct gtaagtccaa taattaaaca tatggatagc ataaatgctg
     3601 ttatttttct aagcattaat taaaacctcc taaatttgta cattcttatc actattacta
     3661 attttatcat acttcaaaga aaaattatat tataaaa
//
LOCUS       DAELLV010000292         3657 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.292, whole genome shotgun sequence.
ACCESSION   DAELLV010000292 DAELLV010000000
VERSION     DAELLV010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.292"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            430..1686
                     /locus_tag="KPD58_003400"
     CDS             430..1686
                     /locus_tag="KPD58_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HBG0267127.1"
                     /translation="MIEKFKTLIWIKMIIKIKSVYKTYGGLFLLGLIGIPIGAIIGLI
                     DTIFGTVLLKVTDIRETYPMYLIPFLAVVGVVIAYCYFKFGGKSSKGMNLIFEVGHGE
                     EEIIPLRLVPFIILGTWLTHLFGGSAGREGVAVQIGATFSHWVGKRLPIKNASSIFLV
                     TGMAAGFAGLFETPIAAILFAMEVLVAGSLEYQSLFPAFTASFTASAVSKALGLEKFS
                     FALSSKVVFDLSIFWKLIVLGIIFGMVGGAFAWCLKLSKRKIGNRLKNPMIRIAIIGV
                     CLSVLFLLFYKGRYSGLGTNLIQNSFYGGEIYSFDWLLKFILTILTLSAGFQGGEVTP
                     LFSIGASLGVLLAGFFNLPIELVAALGYASVFGSATNTFFAPVFIGAEVFGYSYLPYF
                     FVVCAISYIFNMDKSIYSLQKISTKQ"
     gene            1749..2942
                     /locus_tag="KPD58_003401"
     CDS             1749..2942
                     /locus_tag="KPD58_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBG0267128.1"
                     /translation="MLTSLALIFLLGMASGGIFKRIKLPNLLGMLLTGIILGPYVLNL
                     IDNSILDISSDLRKIALIIILTRAGLSLDINDLKKVGRPAVLMCFIPATFEIVGMIVL
                     APKLLGVSILEAAVMGAVVGAVSPAIIVPKMLKLMEEGYGTEKSIPQMLLAGTSIDDI
                     FVIVMFTVFTGLAQGNSISAISFLQIPVSIILGVIVGAVIGICLAVFFKKIHMRDSAK
                     GVLLLSISFLMISLETALEGIVPFSGLLAVMSIGIFLQIKYRVVARRLSIKYSKLWVG
                     AEILLFVLVGATVDISYAFKAGIGAVILIFGVLLFRMVGVFFCLIKTNLTIKERLFCM
                     IGYIPKATVQAAIGGVPLAMGMASGQLILTLAVLAILITAPLGAFGIDVTYKKLLTSV
                     KGENK"
     gene            3143..3523
                     /locus_tag="KPD58_003402"
     CDS             3143..3523
                     /locus_tag="KPD58_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0267129.1"
                     /translation="MCLKKLSKLELVIMKFIWNLDIKTNSYEIIDYMKEEHNLPEKVA
                     LKTLSKLSKKRFLYVQETGKCMYYTVAIKEKAYLEFISRNVQNLLKNNFIRNLLVSFH
                     EEELTEEKIKSLENWVVNWEEAYV"
BASE COUNT         1251 a          388 c          694 g         1324 t
ORIGIN      
        1 ttaatatttt aattgatagg ttatattatt tataacatta aaaatacaat ttacataaaa
       61 tagacatatg aaaaataaaa aattatataa taattatggg aatgaggttc tcccttggat
      121 gataatatcc taaactgcca atgagctgat gacttctatg atatcataga tgccaccagc
      181 tttttttgtt gtttaaatcg ataaaatgga ggtgaattac ttactaaatt tttatttttt
      241 aaatttgaag aaatatttaa tcaataaaaa aaatagtaga ttatatgtta attattttat
      301 taagaatggg ttaagagtgt acctatatcc tatcaattaa aaaaacaatt acgatagtca
      361 ttacattgac tacagttgat gcaataaaac tttcaaaaaa tcttatgatg caaatagaaa
      421 ggaattaaaa tgatagaaaa atttaaaact ttaatatgga ttaaaatgat aataaaaatt
      481 aaaagcgttt ataaaacata tggtggacta tttttgttag gactcatagg tataccaatt
      541 ggtgcaatta taggtcttat agacaccata tttggaacgg ttcttctaaa agttacagat
      601 attcgagaaa catatccaat gtatctgata ccatttttag cagtagtagg agttgttatt
      661 gcttattgct attttaaatt tggaggaaaa agtagtaaag gaatgaatct aatatttgag
      721 gtagggcatg gagaagaaga aattattcct ttaagattgg ttccatttat aatattaggt
      781 acatggttaa cccatttatt tggtggtagt gcaggacgag aaggagttgc tgttcagatt
      841 ggtgcaactt tttcacattg ggtaggtaaa aggttgccta taaaaaatgc ttctagtatt
      901 tttctagtaa ctggtatggc agctggtttt gcaggattat ttgagacacc aatagcagca
      961 atactatttg ctatggaggt tttggtagct ggttcactgg agtatcaatc gctttttcct
     1021 gcgtttactg cttcttttac agcaagtgca gtatcaaaag ctttagggtt agagaaattt
     1081 tcttttgcac tttcttctaa agttgtattt gacttgtcaa tattttggaa gcttattgtt
     1141 ttaggaatta tttttggaat ggttggtgga gcatttgcat ggtgtctgaa attgtcaaaa
     1201 agaaaaatag ggaatagatt aaaaaatcct atgataagaa ttgctattat tggagtatgt
     1261 cttagtgtat tattcctttt attttataaa ggaagatatt caggtttagg gacaaattta
     1321 attcaaaata gtttctatgg tggagaaatt tattcatttg actggctgtt aaagtttata
     1381 cttacaatac tgactttgtc agcaggtttt caaggaggag aggttacacc tttgttttcc
     1441 attggagcta gtcttggtgt attgttagca ggatttttta atttgccaat tgaattagtt
     1501 gcagcgcttg gctatgctag tgtctttggt agtgctacaa atactttttt tgcacctgta
     1561 tttatagggg cagaagtttt tggatacagc tatttacctt atttctttgt agtatgcgct
     1621 atttcatata tatttaatat ggataaatct atttattcac tacaaaaaat ttcaacaaaa
     1681 caataaataa aacaacataa tgtatgatta gaagagaaag aatattagag aaaggatagg
     1741 gtaggaatat gttgacaagt ttagctttga tttttttatt aggaatggcc tcgggaggta
     1801 tttttaagag gataaagtta cctaacttat tgggaatgtt acttacagga attatattag
     1861 gtccatatgt tttaaatttg attgataatt caatactaga tatttcttct gaccttagaa
     1921 agattgcatt gattattata ttaacaaggg ctggattatc tttagatata aacgatttga
     1981 agaaagtggg aagacctgct gtacttatgt gctttattcc agctactttt gaaattgtcg
     2041 gaatgatagt tttagcacca aagttactag gcgtttctat attggaagca gcagtcatgg
     2101 gtgcagtagt aggagcagtt tctcccgcaa ttattgtacc taaaatgctt aaattaatgg
     2161 aagaaggtta tggtacagaa aaaagtattc cacagatgct tttagcagga acttctatag
     2221 acgatatttt cgtaattgtg atgtttacag tattcacagg attggcacaa ggaaatagta
     2281 tatcagctat tagtttttta caaattccag tatcaattat attaggtgta atagttggag
     2341 cagtgattgg aatctgttta gcagtatttt ttaagaagat tcatatgaga gattctgcta
     2401 aaggagtgtt gcttttaagt atctcatttt taatgataag tttagagact gctttagagg
     2461 gtattgtgcc attttctgga ttattagcag ttatgagtat tgggattttc cttcagataa
     2521 aatatcgtgt agttgcaaga agattgtcta taaagtattc gaaactatgg gttggtgctg
     2581 aaattctctt gtttgttttg gtaggggcaa cagtagatat aagttatgca tttaaagcag
     2641 gaattggagc tgtaatttta atatttggag ttcttttatt ccgtatggta ggagtattct
     2701 tctgccttat taagacaaat ttgacaataa aggaaagatt gttctgtatg attggctata
     2761 ttccaaaggc tacagtacag gcagcaatag gaggtgtacc acttgcaatg gggatggcat
     2821 ctggacaact tattttgacg ttagcagttc tagctatact tataactgca cctcttggag
     2881 cttttggaat tgatgtaact tataaaaaat tattaacttc tgtaaaaggc gagaataagt
     2941 aaaaataaag attattttaa ctggtaagtt atattttgga aaaggattat actattgaat
     3001 acaatataaa aaaatcattt gggagattaa gaaggtagaa gttaagaaca gattttaagg
     3061 tatgttatga ttattagaat tgattttgtg aaattattat tgtaatattt aattatatag
     3121 ataaagaagc ggagtgataa ctatgtgttt aaaaaaatta tcaaaattag agttagttat
     3181 tatgaaattt atttggaatt tagatataaa aaccaattca tatgagatta ttgattatat
     3241 gaaagaagaa cataatttac cagaaaaagt agctttaaaa actttatcaa aactatcaaa
     3301 gaaaagattc ttatatgttc aagaaacagg caagtgtatg tattatacag ttgcaataaa
     3361 agaaaaggca tatttagagt ttatatcaag aaatgtgcaa aatcttttga agaataattt
     3421 tataaggaat ttattagtat catttcacga agaggagcta acagaggaaa agataaagtc
     3481 gttagaaaac tgggtagtaa attgggaaga agcttatgta taaatgttat tatttttatt
     3541 acaatttaga taattttggt tttgtaagct ctgcaactat ttttagatgg ttgcagagtt
     3601 tatttttatt ttttattccc ttaagcaata cttaaaagta aacgcattta tatttac
//
LOCUS       DAELLV010000293         3622 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.293, whole genome shotgun sequence.
ACCESSION   DAELLV010000293 DAELLV010000000
VERSION     DAELLV010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3622
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.293"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            313..1689
                     /locus_tag="KPD58_003403"
     CDS             313..1689
                     /locus_tag="KPD58_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HBG0267130.1"
                     /translation="MKTIVRRLILYADGMRPTQIMVSGFAAIIVIGALLLTLPIASQS
                     GESIGLLNALFTATSAVCVTGLVMVDTATYWSLFGQIVIITLIQIGGLGFMTVATMFS
                     LMARKKIQLRERLLIQESLNQADLSGLVRLTRFVLIITITIEGIGALVLSTVFIPQFG
                     LSKGIWYSVFHAISAFCNAGFDLMGSVSGPFTSLNSYVNNFTVSMTVCALIVLGGLGF
                     PVVLDIVRKRRFSKLNVHSKVVLFSTATLIFVGALFIFLIEFNQKATMADLPLKGKVL
                     SAIFQSVTARTAGFNTLDLATLRESSVFVMIILMFIGASPASTGGGIKTTTLAVLIIT
                     VRSFLSGKSDIEAFERRLAPSTIKKSLGIFVISISAVIFGTLIISITQPNFTLVQSAF
                     EVTSALATVGSSLAGTPNLNALGKIIIIIFMFMGRVGSLTLFMAILSGGRRKSQPIRY
                     AEGKIMVG"
     gene            1976..2647
                     /locus_tag="KPD58_003404"
     CDS             1976..2647
                     /locus_tag="KPD58_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HBG0267131.1"
                     /translation="MKQYIVIGCGRFGSSVASTMHLLGHQVMAIDKNEDSVQSISDKV
                     THSLIVDVTDEQALRSLGLGNFDVAVVAIGSDIRASIMATLIAKEMGVELIICKAKDE
                     LQAKVLYKIGADRVVFPERDMGVRVAHNLVSDNILDHIELDPEYSIVEIVTPNSWVGK
                     TLIELELRARYEITVLAIKTGKNINVTPSPDEELTAGSILVIIGQNTSITAITSGNKG
                     IIRRR"
     gene            2836..>3622
                     /locus_tag="KPD58_003405"
     CDS             2836..>3622
                     /locus_tag="KPD58_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HBG0267132.1"
                     /translation="MITNINSKDNEKLKYTRALLKSKNRNKESKFIIEGYRIVMLALE
                     CMANLDYVFINEEFENKKEHVKLLEDLDKKNTKIYKTTNKNFKELVDTENTQGIIGVV
                     SFKKKKLSESINKKDKFVLILDRIQDPGNMGTIIRTADSAGVDAIIALKGCVDIYNPK
                     VIRSTMGSIFDMNIIDASQDETVDMLKSLDFNIVSSYLNTENFYDKIDYGSKVALVIG
                     NEANGINEELVSKSDILVKIPIYGKAESLNAAISSAILMYEIKK"
BASE COUNT         1375 a          405 c          671 g         1171 t
ORIGIN      
        1 atttaacttc aaaaaagtat atgaaattta tactggtaaa atgataaata aattttaagt
       61 acaaaatagt aaaagctatg atagttttag acctattata tgtaaaaaat atagatgttt
      121 ttggtgcaat ttaaattaaa agatatatat aaaaatttaa aatattccaa acagaaatta
      181 taaaaattac tatcattgtc tttatacaat atttatacta taatagaata ataatttaga
      241 ggaaagctct aaaaataaac aaattaaggg tcttaacagt agtgatttta tgttaaggag
      301 gtataaagaa agttgaaaac aatagttaga agacttattc tctacgcaga tggaatgcgt
      361 cctactcaaa taatggtatc tgggtttgct gcaattattg taatcggagc attgttgtta
      421 acactcccga tagcgtcgca gagtggagag agtataggct tattaaatgc tctatttacg
      481 gctacttcag cggtttgtgt tactggtcta gttatggtag atacagcaac ttattggagt
      541 ttatttggac agatagttat aataacattg atacaaattg gtggtcttgg atttatgacc
      601 gtagccacaa tgttttcact tatggcacga aaaaaaatac aactaagaga aagattatta
      661 atacaagagt cattaaatca agctgattta tcgggacttg taagacttac aagatttgta
      721 ttaataataa ccataacaat agagggaata ggtgcactgg ttttatcaac tgtctttatt
      781 ccacaatttg gtttgtctaa agggatatgg tatagtgtat ttcacgcaat atcagcgttt
      841 tgtaatgccg gatttgactt gatgggcagt gtaagtggcc catttacatc gttaaattct
      901 tatgtaaaca actttacagt atcaatgact gtttgcgcac ttatagtgct tggagggcta
      961 ggattccctg ttgtacttga tattgtaaga aaaagaagat tttcaaaatt aaatgtgcat
     1021 tctaaggttg tattgttttc aactgctaca cttatttttg tgggtgcatt atttattttt
     1081 cttatagaat ttaatcaaaa agctactatg gcggacttgc ctttaaaggg aaaagtacta
     1141 tcagcaatat ttcaatcagt aacagcaaga actgctggtt ttaatacact tgatttagct
     1201 actttacgag aaagtagtgt atttgtaatg ataattttga tgtttattgg agcatcacct
     1261 gcatcaactg gtgggggtat aaaaacaaca acactagctg ttttaatcat tacagttaga
     1321 agttttctat caggaaaatc agatatagaa gcttttgaaa gaagattagc tccatcaact
     1381 ataaaaaaat ctcttggtat atttgtaatt agtatatcag cagttatttt tggaacattg
     1441 attatttcaa taactcagcc taattttaca ttagttcaat cagcttttga ggttacatca
     1501 gcacttgcta cagttggttc aagtttagct ggaactccaa acttaaatgc tttaggtaag
     1561 attataataa taatttttat gtttatgggt agagttggtt ctcttacttt atttatggct
     1621 atactttctg gtggtagaag aaagagtcaa ccaatcagat atgcagaagg taaaattatg
     1681 gttggttaag ttaccaagga gtagtataaa tttataatgg atttagagta gcactgtaaa
     1741 tctgttgata aaataaatga gcctagatgt aattatatga agaaataaaa tcaactaacg
     1801 aacaaaatta attaatgaaa taaaaaaaca gggaaataaa atacaaataa aaaggtaaat
     1861 caaaattaaa ttaatctgtg tatataaaat tatatattgt gtataagttt tgtatgtgta
     1921 aacaattaat ttaagagata ttatataaat tgtgtattag ggaggaagtt tcgagatgaa
     1981 gcaatatata gtcattgggt gtgggagatt tggaagttca gttgcgtcta ctatgcatct
     2041 tttaggacat caagtaatgg caatagacaa aaatgaagat tcagttcaaa gtatatctga
     2101 caaggtaacc cattcactta tagtggatgt tactgatgag caagcgttaa ggtcattagg
     2161 tttaggtaac tttgatgtag cagtagttgc aataggttct gatataaggg catctataat
     2221 ggcgactctt atagccaaag aaatgggtgt agagttgata atatgtaagg caaaggatga
     2281 attacaagct aaagttcttt ataaaattgg tgcagataga gttgtatttc cagaaagaga
     2341 tatgggagta agagttgcac acaatttagt ttcggataat atattagacc atattgaact
     2401 tgacccagag tattcaattg ttgaaatcgt aactccaaat agttgggttg gcaagacact
     2461 tatagagctt gaattaagag ctagatatga gataactgta cttgctataa aaacaggtaa
     2521 aaatataaat gttacacctt ctccagatga ggaacttaca gccggaagta tcctagttat
     2581 aatcggtcaa aatactagta taacagcgat aacatctgga aataagggga taattagaag
     2641 aagataattt actatttaat atatatttaa ttgcaatgaa agtagatagt atcatataat
     2701 tatgaatagt tatatgatac tattttttta atcgaaggta gtatttattt gtaagattag
     2761 ataaagagaa gttaaattaa aagtaaggag gctgtgctaa tataaaaatt tataagttat
     2821 tagcatttag ataaaatgat tacaaatata aacagtaagg ataatgaaaa gttaaagtat
     2881 acaagagcac tattaaaatc aaagaatagg aataaagagt caaagttcat aatagaggga
     2941 tacagaatag taatgcttgc acttgaatgt atggcaaacc ttgattatgt atttatcaat
     3001 gaagaatttg aaaataagaa agaacatgta aaactattag aagatttgga taaaaaaaac
     3061 acaaagatat acaagactac taataaaaac tttaaagaat tagtggatac agaaaatact
     3121 caaggaataa taggtgtagt ttcatttaag aaaaaaaaat taagtgaaag tataaataaa
     3181 aaagataaat ttgtattgat tttagacaga atacaagacc caggaaatat ggggactata
     3241 ataaggactg ctgattctgc tggagtagat gccataatag cactaaaggg atgtgtcgat
     3301 atatacaacc caaaagtaat taggtctact atgggttcta tttttgatat gaatataatt
     3361 gatgcttcac aagatgaaac tgtggacatg cttaaatcat tggattttaa tatagtttca
     3421 agttacttaa atacagaaaa tttttatgac aaaatagatt atggctcaaa agtagcattg
     3481 gtgataggaa acgaagcaaa tggaataaat gaagaacttg tatcaaagtc tgatattttg
     3541 gttaagatac ctatatatgg taaagccgag tcgttaaatg ctgcgataag ctctgctata
     3601 ctgatgtatg aaataaaaaa at
//
LOCUS       DAELLV010000294         3595 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.294, whole genome shotgun sequence.
ACCESSION   DAELLV010000294 DAELLV010000000
VERSION     DAELLV010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.294"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            2..745
                     /locus_tag="KPD58_003406"
     CDS             2..745
                     /locus_tag="KPD58_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0267133.1"
                     /translation="MLKIKNISKDYGIKGFKTNVLKNISLTVNEGDFISIMGPSGAGK
                     TTLLNLMSTLDKQTSGEIILDGINISKVKNNELSKLRREKIGFIFQDYNLLDNMKLMD
                     NIALPLALGKKKSKEIEAKVFSIAKKFGLENHLDKYPYQLSGGQKQRGAAARSLITNP
                     TVIFADEPTGALDSKSAYELLESLEKINRENNATIIMITHDPLTASYSNEVYMINDGN
                     IKCKLNKGNSRKEFYGKIMDMLASMGGEM"
     gene            746..2649
                     /locus_tag="KPD58_003407"
                     /pseudo
     CDS             746..2649
                     /locus_tag="KPD58_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891367.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            2801..3481
                     /locus_tag="KPD58_003408"
     CDS             2801..3481
                     /locus_tag="KPD58_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0267134.1"
                     /translation="MYRILLVEDDIDLSKEIALALEKWGFKVELIDDFEVVLDEFIDK
                     KPDVVLLDVNLPLYNGFYWCEKIRAISNVPLIFLSSRDSDMDLIMGINNGADDYITKP
                     FSIEILVTKINGIIRRVYNYSDSNSILYCEDLMFDVGKGIIKHKYKDKSIELTKNEIK
                     ILTLLLKNKNRVVSRESLMMTLWDNDEFVTDNALTVNMNRLRSKVKELGFDDFIKTKK
                     GIGYIIQC"
BASE COUNT         1500 a          319 c          602 g         1174 t
ORIGIN      
        1 aatattaaaa ataaaaaata tatcaaaaga ttatggaata aaaggattta aaacaaatgt
       61 gttaaaaaat atatctctta cagtcaatga aggtgatttt atatcaataa tgggtccatc
      121 tggtgcagga aagacaacat tgctaaattt aatgtctaca cttgacaaac aaacttcagg
      181 agaaataata ctagatggaa ttaatatatc aaaagtaaaa aataatgaac tatcaaaatt
      241 aagaagagaa aaaatagggt ttatatttca agactataat ctattagaca atatgaaact
      301 aatggacaat atagcacttc ctctagcatt aggaaaaaag aaaagtaagg agatagaagc
      361 taaggtattt tctatagcta aaaaatttgg attagaaaat catttagata aatatccata
      421 tcagttatca ggaggacaaa aacaaagagg cgcagcagct aggtcattaa ttacaaatcc
      481 aacagtcata tttgctgatg agccaactgg tgctttagac tctaaatcag catatgaact
      541 tttagaatca ttagaaaaaa taaacagaga aaataacgct actattataa tgattacaca
      601 tgaccctcta acagcaagtt attcaaatga agtatatatg ataaatgatg gaaatataaa
      661 gtgtaaatta aacaaaggaa atagtagaaa agaattttat ggaaaaatta tggatatgct
      721 agcctctatg ggaggtgaga tgtaagtgag tatacttgga attgcttata ataattttaa
      781 gagtaacata agaacttatc tagcattttt tatatctatg gttttttcag tagttgtgct
      841 aactaatttt gaattattaa gatatggtga tgctataaat gtgttacaag atgaaaataa
      901 aaaatttaca ctatcagtgt tagtagctgt aataatgata ctatcagtat ttttattttt
      961 ctttatatgg tatgcaacaa atatattttt taagaatagg tctaaagaaa ttggaatatt
     1021 gtcttttatg ggattagatt tatatactat aggaaaaata tattttatag aaaatatgct
     1081 tataggaata agttcttgta taacaggaat aattataggg ataataacat ctagattttt
     1141 tcaagtagtg ataataaagt tatctggatt taatataaag gtatctaatg gacttagtat
     1201 agaagctata ctatatgcta ctttaatctt tatttctata tttataataa tggctgtaaa
     1261 aggttttata acaatatgta gaagtagtgt aataaatcta atcaatatat ctaaaaaaca
     1321 agaaaaaata ccaaaggtag gaataggtat atatatatta gctatagtat caattatagt
     1381 aataggatat ggctactatc tttctaccaa tataagaact ggagatataa ctagtgtact
     1441 acctgttata ggtataataa tagtaggtac ttatggatta ttcaaatcag ttatgcctgt
     1501 tatatttgat attataatca aaaataaaaa aatattattt aatggaaata atataattgc
     1561 aataaacaat ataaactacc gattaaataa aaactataaa acttatgcta taatagctat
     1621 aattataact acaacaatat caactcttgg tgcttcagtt gcgatgaagc gtatacataa
     1681 gaatgcccaa gaacaaagaa atgtgtacac tattagcata gtttcaatgg ataaagatga
     1741 agtgaataaa aaacaaatag agaatataat aaaagatact aataagataa agtactcagt
     1801 aaatccagaa ctaacaattt taaagaaaac agatataaaa gaagcttatg atacaacaga
     1861 tactataatg aaatatagtg atttttgtaa tactcttaaa gtgaatggca atgataaaga
     1921 attaaaagag tatggtgaaa gattagttag tggaaataat gtaatagagt taaaaagccc
     1981 tcaaacgata gcatcaatag ctacaaaaga agccaagata aaacttgatg gtcagttata
     2041 taatatttca aggggagaag taaaagttcc agtactaggg acaggactta atcaaagtat
     2101 tatagtggta aatgatgata catattttaa actaagagat aagggtcaac aagtgtattt
     2161 ttatggtgca aaggtatttc aagaagaaaa tagtcaagta atgttcaaca atattgaaaa
     2221 gaatttaagt ttaaaaaatg tgtatattca aaatggatat aaagctcaag gtgagagtca
     2281 atggatgaag tttgcatatg tagtgtgttt ttattcttag tctttgcaat tgtagctgga
     2341 agtataatat atatgaaaat atatagtgat gcatatgaag ataaagagaa atataagatt
     2401 ttgttaaaaa taggtactac agaaaaagat ataaatagag ctatacttaa agaagtagct
     2461 attttttata ctttgccaat gttaagtgca actatatcta gttattttgc attgagatta
     2521 gcaggagatt tattgatgac cgatttattt ggtatatata tactaagttt agtgatatgt
     2581 ttgataatat tcataatata tggagcagta tctgttaata agtttaagag tgtagtgtat
     2641 aaaaattagt tgatatatta ataaagagaa aggagattct tgtaataaga gtctccttgt
     2701 tgattaacta aataatttgg gtaataaatt catataaaat atgttatgat attaataatt
     2761 catagcatag tgaaatatta ggataatgga gaatatacaa atgtatagaa ttttattagt
     2821 agaggacgat attgatttat caaaagagat tgcattagca cttgaaaaat ggggatttaa
     2881 agtagaatta attgatgatt ttgaggtagt attagatgaa tttatagata aaaaaccaga
     2941 tgtagtactt ttagatgtaa atttaccatt atataatgga ttttactggt gcgaaaaaat
     3001 aagagctatt tcaaatgtac cattaatatt tttatcatca agagattctg atatggattt
     3061 aataatgggt ataaataatg gagcagatga ttacattacc aaaccatttt ctatagaaat
     3121 attggttacc aaaataaatg gtataataag aagagtctat aattatagtg attctaatag
     3181 tattttgtat tgtgaagatt tgatgtttga tgtggggaaa gggattataa aacataagta
     3241 taaggataaa agtatagagt tgactaaaaa cgaaataaaa attttaacat tattattaaa
     3301 aaataagaat agggttgtat caagggaaag tcttatgatg actctatggg ataatgatga
     3361 gtttgtgaca gacaatgcac ttactgtaaa tatgaataga ttgagaagta aagttaaaga
     3421 actaggtttt gatgatttta taaaaactaa gaaggggata ggatatataa tacaatgcta
     3481 gtaaaatatt tgaaaggtag gtctaagtat attgctctac ctttagttgt agttagtata
     3541 ataaatatct acttatttgc tatagatatt tttaaaaata aatattcaga gttaa
//
LOCUS       DAELLV010000295         3576 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.295, whole genome shotgun sequence.
ACCESSION   DAELLV010000295 DAELLV010000000
VERSION     DAELLV010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.295"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(50..589)
                     /gene="scpB"
                     /locus_tag="KPD58_003409"
     CDS             complement(50..589)
                     /gene="scpB"
                     /locus_tag="KPD58_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HBG0267135.1"
                     /translation="MKREDIKYIIESVMFAYGEPISIKELNYIINKELSSKEIEIMLN
                     LLIEEYREQNRGIQIIKLENKYQMCTNKDYAEYIKKIIEPKKKKSLSQATLETLTIIA
                     YKQPITKVEIEDIRGVKCDKVLQTLFENELIREAGRLNKIGKPIIYKTTDEFLKLLNI
                     ESLEELPPIENYQEVATNE"
     gene            complement(591..1310)
                     /locus_tag="KPD58_003410"
     CDS             complement(591..1310)
                     /locus_tag="KPD58_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HBG0267136.1"
                     /translation="MKYNIQLQVYEGPLDLLYDLITKHKIDIKDISIIDITKQYLNYL
                     KMLDKMDLEITSEFITMASKLLEIKSKYLLYKQKDEEEDPRIELMEKLEEYRKFKVAS
                     QDIKENITYVNERFYRNKEEIIIDDNVDLEDISIEAIKNILPYIFKVKTSQIENANDE
                     KLDKIVRKKIISVEEKILYIRDIIKDKIEVTFTNIIKSYENDEIIATFLSILELIKEK
                     EIVVVQDIFFDDILIRKSSEC"
     gene            complement(1340..1936)
                     /locus_tag="KPD58_003411"
     CDS             complement(1340..1936)
                     /locus_tag="KPD58_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HBG0267137.1"
                     /translation="MLSMNIGEIVASLVGIAMAISIHEFGHAYSAHLLGDDTAKAYGR
                     MTLNPVRHVDPIGLLAMFILKIGWAKPVPVNPNNFKNYKIGNLIVSLAGVFCNVLTAI
                     LCVIINKYVHMYAINTIAGYVLLYSMGFAAFNLLPIPPLDGWGVISTFVPYKWNEYLY
                     KFESMSYIILLIALFTGMYRIILSPIYTVLLKIVYLFV"
     gene            2234..3070
                     /locus_tag="KPD58_003412"
     CDS             2234..3070
                     /locus_tag="KPD58_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG0267138.1"
                     /translation="MRQEERTVCFDRELKIEAYRFKGIMQKFPNHFHEHYVIGFIEKG
                     QRYLSCKNKEYTTSTGDLLLFNPFDSHTCEQIDDKVLDYRCINIKPEIMKKTVFEITG
                     KNYLPKFNQPVIFRSELVPLLQELHYIIMEEELDFKKEELFFFLIEQLIEEHTEPNLQ
                     SNLENTNIEIQAVCDYLENNYAEHIVLDELSTIAGMNKYSLLRNFTKLKGITPYRYLE
                     NIRVNKAKKLLEKGVEPIDAAIQTGFVDQSHFTNFFKNFIGLTPKQYQNIFINDDSNN
                     FK"
     gene            3091..3513
                     /locus_tag="KPD58_003413"
     CDS             3091..3513
                     /locus_tag="KPD58_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HBG0267139.1"
                     /translation="METKNSKCVMVIDENLPMGIISNTAAIMGITLGKHAPETVGPDV
                     IDKTGNSHLGIIDIPVPILKGNKEIIKDLRKKLYTLEFNDLTVVDFSDVAQSCNLYEE
                     FTQKIASVPEDELQYFGIAIYGNKKKVNKLTGSMPLLR"
BASE COUNT         1274 a          529 c          413 g         1360 t
ORIGIN      
        1 atcttttaaa atttaaaatt acatactaaa ttattgtgat tttatatttt tattcatttg
       61 ttgccacttc ttgataattt tctataggtg gcaattcttc aagactttct atattcaaca
      121 actttaaaaa ttcatctgtt gttttataaa ttattggttt ccctatctta ttcaatctac
      181 cagcttctct tataagttca ttttcaaaca aagtttgtaa taccttatca cattttactc
      241 ctcttatatc ctctatttct acctttgtta ttggttgttt gtaggcaatt atagtaagag
      301 tttctaatgt agcttggctc aaacttttct ttttctttgg ttcaataatc ttctttatat
      361 attctgcata atctttattt gtacacattt ggtacttatt ctctaacttg attatttgaa
      421 tacctctatt ttgttctctg tactcctcaa taagtaaatt taacataatc tctatttctt
      481 ttgatgaaag ttctttattt attatatagt ttaattcttt tatacttatt ggttccccat
      541 atgcaaacat aacagattct ataatatact ttatatcttc tcttttcatt ttaacactcc
      601 gaactttttc ttattagaat atcatcaaaa aatatatctt gtactacgac tatttctttt
      661 tcttttataa gctctaatat agataaaaaa gtagctatta tttcatcatt ttcatatgat
      721 tttataatat ttgtaaaagt aacttcaatc ttatctttta taatatctct tatatataat
      781 attttttctt ctacagatat tattttcttc cttactattt tatctagttt ctcatcatta
      841 gcattttcta tctgtgatgt tttaacttta aatatgtatg gaagtatatt ttttattgct
      901 tcaattgata tatcttctaa atccacattg tcatcaataa ttatctcttc tttatttcta
      961 taaaatctct cattaacata agttatattt tcttttatat cttgtgatgc taccttaaac
     1021 tttctatatt cttctaactt ttccataagt tctattcttg ggtcttcttc ttcatctttt
     1081 tgcttataaa gcagatattt agactttatc tctaaaagtt ttgatgccat agttataaat
     1141 tcactagtta tctctaaatc cattttatct agcattttca aataatttag atactgctta
     1201 gttatatcta ttatggatat atcctttata tctattttat gctttgttat taaatcataa
     1261 agtaaatcta atggtccctc atagacttgt aattgtatat tatacttcat atcaatccct
     1321 ctttaatctg tttattatat taaacaaata aatatactat ttttagcaac actgtatata
     1381 ttggtgatag tattattcta tacatcccag taaataaagc aattaataaa attatataac
     1441 tcatactttc aaatttatac aaatattcat tccatttata tggtacaaat gtagatataa
     1501 ctccccatcc atcaagtgga ggtatcggaa gtaagttaaa agctgcaaat cccatactat
     1561 ataaaagcac atatccagct attgtattta tagcatacat atgcacatac ttattgataa
     1621 ttacacacag tatagcagtc aaaacattac aaaaaacgcc tgcaagtgaa actatcaggt
     1681 ttcctatctt atagttttta aagttatttg gatttactgg tactggcttt gcccacccta
     1741 tcttaaggat aaacatagcc aaaagaccta ttgggtcaac atgtcttaca ggatttaatg
     1801 tcattctacc ataagcttta gctgtatcat cccccaataa gtgagctgaa taagcatggc
     1861 caaactcatg tattgatatt gccatagcta taccaactaa acttgctact atttctccta
     1921 tattcatact taacattaat gtacatctcc taaattaaat ttttaatatt attataacaa
     1981 ttaaatagct tataatcaaa attattttac attataataa ttttaacttc taggcataaa
     2041 attaaaattt gctttttatt ttataacttt tattattata tcatattcat tgtgaaaata
     2101 taataattac acctactgta agtataatat tatattgaca tgaattgaca ttattgtctc
     2161 ttagtcaata ttatacaaga agtataaaaa aatatgtgtt acactttgta taacatgaaa
     2221 ggaggttacc taagtgagac aagaagaacg cacagtatgc tttgataggg aattaaaaat
     2281 tgaagcatat agatttaaag gcataatgca aaaattccca aaccattttc atgaacatta
     2341 tgtaattggt tttatagaaa aaggtcaaag atatttatct tgtaaaaata aagagtatac
     2401 cacaagtact ggagatttgc tactttttaa cccttttgat agtcatactt gtgaacaaat
     2461 agatgataaa gtacttgatt acagatgtat taatattaag ccagaaataa tgaaaaaaac
     2521 cgtctttgaa attacaggta aaaattattt accaaaattt aatcaacctg ttatatttcg
     2581 cagtgagcta gtacctctac tacaagaatt acactatata atcatggaag aagaattgga
     2641 ttttaaaaag gaagagcttt ttttcttcct aattgaacaa ttaattgaag aacatacaga
     2701 gccaaatttg caatctaact tggaaaatac aaatatagaa attcaagcag tatgtgatta
     2761 tctagaaaat aattatgctg aacatatagt acttgacgaa ttaagcacta tagcaggtat
     2821 gaataagtac tctctacttc gtaattttac gaaattaaaa ggtattacac catatagata
     2881 cctagaaaat atacgggtaa acaaagctaa aaagctttta gaaaaaggcg tagaacctat
     2941 agatgctgcg atacaaacag gatttgtaga ccaaagtcac tttacaaact tttttaagaa
     3001 ttttattggt cttacaccaa agcagtatca aaatatattt ataaatgatg attcaaacaa
     3061 ctttaaataa ggtaaaaagg agaaataaaa atggaaacta aaaattcaaa atgtgtcatg
     3121 gtaatagacg aaaatttacc aatgggaatt atatccaata cagcagctat aatgggaatt
     3181 acactaggaa aacatgcccc agaaactgtt ggcccagatg taattgacaa aacaggtaat
     3241 agccacttag gaattattga cattcctgta cctattttaa aaggaaacaa agaaataata
     3301 aaagatttac gtaaaaaatt atatactttg gaatttaacg atttaactgt tgtagatttt
     3361 tcagatgtag cacaaagttg caatctttat gaagaattta cacaaaagat tgctagtgtt
     3421 ccagaagatg agttacagta ttttgggata gcaatttatg ggaataaaaa gaaagtgaat
     3481 aaacttactg gtagtatgcc tttacttaga taattgttaa acaaccctat atatgatatt
     3541 aaatcaaatg atattttaag atacataaac ttttct
//
LOCUS       DAELLV010000296         3562 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.296, whole genome shotgun sequence.
ACCESSION   DAELLV010000296 DAELLV010000000
VERSION     DAELLV010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.296"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(326..1597)
                     /locus_tag="KPD58_003414"
     CDS             complement(326..1597)
                     /locus_tag="KPD58_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG0267140.1"
                     /translation="MLKVLKGNIIFTKTSETFTVFENSYIILEKGKVKGIFKEIPKEY
                     KNLKVVDYTDKLIIPGMNDLHAHASQFKNLGMAMDKELLPWLETYTFPEESNYKDIEY
                     ATKMYKKFVKELWKSGTTRIAVFATVHKEASMKLMDIFKEVGLGAYVGKVNMNMNCPD
                     TLLENTQDSITDTEEIINKYSDTDSIVKPIITPRFIPSCTSELLKGLGDLCLKYNIPI
                     QSHLSENYDEIEWVRSLEPESKFYGDAYNRYNLFGQTPTLMAHCVHCSQDEIDLMREN
                     NVMAVHCPASNFNVGSGAMPIRKLIDNNIRLALGSDISGGHTLSIFKAMVSAIQLSKL
                     YWVNSGKKYNFLSLSETFYIATKSGGSFFGKVGSFEEGYDFDALIIDDSNLNHDNYSI
                     LERLERFIYVGDDRNIIHRYVCGKLIEEPNI"
     repeat_region   2244..3459
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=2244..2272
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaat"
BASE COUNT         1274 a          420 c          463 g         1405 t
ORIGIN      
        1 ggagattaga agtgtttgat aattacagta catgttagat atatcattgt aaattaacat
       61 agctttgaat ataataatgt tcaaaaggca atatttttga ggtctgaatc caataataat
      121 atagtcttat gattgtagat atcaaaaaat ataagttact aagaatacgt tttgtagatt
      181 tatatatgat taataactaa agctataaat aaacaaaata aaaagagtat gtttcaacct
      241 atagctttta gatttaaaat atactctttt attaattata gttgtataaa ttaaccataa
      301 tatataatta aaatttttat tataattata tattaggttc ttcaattagt tttccacata
      361 catatctatg aatgatattt ctatcatcac caacatagat aaatctttca agtctttcaa
      421 gtatagaata attgtcatga tttaaatttg aatcatcaat aatcaatgca tcaaaatcat
      481 aaccttcttc aaagctacca acttttccaa agaaacttcc accacttttg gttgcaatat
      541 aaaaagtctc agataaagat aagaaattat acttcttacc agagttaacc caatataatt
      601 tagataattg tattgcagat accattgctt taaatattga caatgtatgt ccaccactta
      661 tatctgatcc aagtgctaat ctaatattat tatcgattaa ttttctgata ggcatagcac
      721 cactaccaac attaaagtta gaagctggac agtgaactgc cataacatta ttttctctca
      781 ttaaatctat ttcatcctga gaacaatgaa cacaatgagc cattaaagta ggagtttgac
      841 caaataaatt atatctatta tatgcatctc catagaattt agattcaggt tctagacttc
      901 taacccattc aatctcatca taattttcag ataagtgtga ttgaatagga atattatatt
      961 ttaaacataa atctcctaaa ccttttaaaa gttcacttgt acagcttggt ataaatctag
     1021 gtgttattat tggttttaca atagaatctg tatcactata cttatttata atttcttctg
     1081 tatcagtaat agaatcctga gtgttttcta ataaagtatc agggcaattc atattcatat
     1141 ttactttacc aacgtatgct ccaagaccaa cttctttgaa tatatccata agtttcatag
     1201 aggcttcttt atgtactgtt gcaaacactg ctattcttgt agttccagat ttccaaagtt
     1261 cttttacaaa ttttttatac atttttgttg catattctat atctttataa tttgattctt
     1321 ctggaaatgt ataagtttca agccatggca aaagctcttt atccatagcc attccaagat
     1381 ttttaaattg agaggcatgg gcatggaggt cattcatacc aggtattata agcttatcag
     1441 tgtaatctac tacttttagg tttttgtatt ccttaggtat ctctttaaat attcccttta
     1501 ctttaccttt ttccagtata atataactat tttcaaatac tgtgaaagtt tctgaagttt
     1561 ttgtaaaaat tatatttcct tttaatactt ttaacatata ggtctcctta gttttaaatt
     1621 tgttttaata tatgtaggta aaattttgtt ttacaaatca aaattttacc acatgtatta
     1681 tatcaaatct aaactataaa tactattaga taatagcaaa tattagtgaa atacagttgt
     1741 ttactattat tatatataac tgacatttaa gtgacattta agaaaaatat aatgcctact
     1801 tacataaaat ggaatgttat ttaaagagaa ctttgattat attttcagaa gcttttttat
     1861 ccatatcgtt taaaatatga gaatatctat ttatagttat ttttatatta gtatgtccta
     1921 atctttcaga gatgattttt atattagttc tagctagaag aagaactatt agaatagata
     1981 atatagtaag tatttacaaa tatgtaggtg ttcttaaatt gataaattat tccattttaa
     2041 ttttatagtt tgaattttat gatataataa aaatatataa attttgcagt gagcgatatt
     2101 tttgataaag tagggtttaa cagttgcaat gtaagggatt gagggtgtat gataaatgtt
     2161 atcaattgca ctactcatgg ttcactgcaa atttgagaga gttgtatgtg tgtaagtact
     2221 gaaaatactt agtttatttt ggggttttat attaactaag tggtatgtaa atagaattta
     2281 gtactttcta aaaatggatt gcctagcaag ttttatatta actaagtggt atgtaaattt
     2341 tcctaattcc ctatggtgta gcattggttg atagagtttt atattaacta agtggtatgt
     2401 aaatatatta aagatatgac actcttttat ataaaatata agttttatat taactaagtg
     2461 gtatgtaaat acaagttcag tagggagaca gacagtaatt ttaaaagagt tttatattaa
     2521 ctaagtggta tgtaaatcac aacaatacaa ataaaactct gaaaaggtaa aagttttata
     2581 ttaactaagt ggtatgtaaa tttattatta ttatatatct tgacttagca ctatttttgt
     2641 tttatattaa ctaagtggta tgtaaatatt actttgaaag cattaacaga aaaagagatt
     2701 agtaagtttt atattaacta agtggtatgt aaattattta ctgttgaacc tacatcttga
     2761 ttggataaaa agttttatat taactaagtg gtatgtaaat taaggtttta atttattagt
     2821 aataaatata agacctagtt ttatattaac taagtggtat gtaaataatt ctttgactgc
     2881 atcatttaaa taatctagct gagttttata ttaactaagt ggtatgtaaa ttctaaaagg
     2941 tcacatatat ttattaaatc tgttgccggt tttatattaa ctaagtggta tgtaaatcat
     3001 ataatgaatt acataacatc ttatgatatt catcgtttta tattaactaa gtggtatgta
     3061 aatttcatag tctttagagt ctgtaaatac tttactcata gttttatatt aactaagtgg
     3121 tatgtaaatt tactttaaaa tatgtaccat aaaaaaatat atcaagtttt atattaacta
     3181 agtggtatgt aaatatctct ggcgtataac cttcttttcc tattagttct ggttttatat
     3241 taactaagtg gtatgtaaat agggtttctg ataaaatctt caaacatgta aaatatgtgt
     3301 tttatattaa ctatgaggta tgtaaatctt atacttagtt agaactatat atcgacacaa
     3361 atatgcttta tattaactat atggtatata aattgcaatt tttattcgtt gtccaatcgc
     3421 tttgaaattt gttttatatt aactaagtgg tatgtaaatt tgcaacaagt ataggtaaaa
     3481 taccccaata atttatacag cattttctcc tttaaaatat aattattttt tatcatttgt
     3541 agtaaataat taccacataa ta
//
LOCUS       DAELLV010000297         3546 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.297, whole genome shotgun sequence.
ACCESSION   DAELLV010000297 DAELLV010000000
VERSION     DAELLV010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.297"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            227..856
                     /locus_tag="KPD58_003415"
     CDS             227..856
                     /locus_tag="KPD58_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0267141.1"
                     /translation="MNKYLLINPVAERMYGEKFHLIKANLIKKGYIIAECEPQLEYVK
                     KQYKEYTKKTKNTMLDCRCPESISLLKRNNLINDFEVPMIEPILVRTCRVLYDKYIKS
                     KDDMLVVTCPCTQLRDFASEKFKDKGNAMFYTWKEFAEQEGVKSLGKIDESPIPLGYF
                     KDTFEKVLEVSSEDEILSEIQKIRNGFEDKYDIVEMFYCKDGCNNGDGL"
     gene            857..1639
                     /locus_tag="KPD58_003416"
     CDS             857..1639
                     /locus_tag="KPD58_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0267142.1"
                     /translation="MVNKFNNKFLRLVKSVLIFFIILLLWKITNYLGIWSDYILPSPE
                     KVYSTFLNMISDGSIFINVYASMKRVLIGFAISTAIGVPLGIFFGIYSGVYEYFKSLI
                     NFLRNTPPLALIPMLILWFGIGEESKIIIIVLASFFPIFTSTLKGIKNCDSKLIEVGR
                     VFEFSKLQIIFKIIIPNAILDIAVGLKLALGYSFRAIIGAELVAASSGLGYLISDGKE
                     MSRTDVVIVGIIVIGLLGIITDYIFSIIVKKVSKGKMVEAYE"
     gene            1632..2510
                     /locus_tag="KPD58_003417"
     CDS             1632..2510
                     /locus_tag="KPD58_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0267143.1"
                     /translation="MNNLVCDSGYIIRNLYKSYKVDNEEHVVLSDISLNIDRNHITVI
                     LGESGCGKTTLLRAIASLEGINSGEIRFINDNKEYRPNVGFVFQESRLMPWLNVSENI
                     AFHNQRKNTILNKVLRKFKHNKRISSKIINDNFRSLETNDNLNKSNKYNRKSNIDVEK
                     YLKMMNLQKFKNSYPNELSGGMAQRVSIARALSFNPDMLLMDEPFSALDYFTRIDMQN
                     EVIRIHKSTNKGVIFVTHDIDEALKIGKKIVVFTDERKIKEFNIEDGYNRDLTSDYYI
                     KLKKDILMTMKSNKGI"
     gene            2543..3502
                     /locus_tag="KPD58_003418"
     CDS             2543..3502
                     /locus_tag="KPD58_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG0267144.1"
                     /translation="MKLKKLLIGITILTLATASLAGCAKKNNGEKLSEINLTYVKSPL
                     NVPSIIQKQDDLFGKEFKKDNIKVNFHEITTGPEQTQALAAGEIDFLHALGGTSALIA
                     ASNGVELKILNTYSRSPKGFMILTNNNSIKSAADLVGKKVAGPKGTILHQVLISALDK
                     EGLSMDDVEFVNMGIPEASAALSDGSVDAALIAGPAALKAMKSGSKLVANGEGLVDGI
                     IVTAVSTDFAEKHPEIVERFMKVEKETLEYVNNNFDEAMEKVAKEVDLSLEETKELYA
                     WYDFSLDITDKDISSLEDTQDFLIKNKLQEKKVNIKELIYNAK"
BASE COUNT         1422 a          345 c          642 g         1137 t
ORIGIN      
        1 ttattatata atatttatag attattatat aagtagatta ttagtaagat gtatgcatta
       61 ttaattaaaa taaattacat atactattta atatttcatt taaaatatta aaaaatgtag
      121 ataaaaattg ttttattaag aaaaaattaa tatgttgaca aaatttaagt gctcatttaa
      181 actattaatt gataataata ttcaatatta aataatagga ggcgttatga ataaatatct
      241 tctaataaat ccagtggcag aaagaatgta tggagaaaag tttcacctta ttaaagcaaa
      301 tttgataaaa aaggggtaca taatagcaga atgtgaacca cagttagaat atgtaaaaaa
      361 gcagtacaaa gagtatacaa aaaagactaa aaatacaatg ttggattgta gatgtccaga
      421 aagtataagt cttttaaaaa gaaataattt gataaatgat tttgaagtac ctatgataga
      481 accaatatta gtaaggactt gtagggtttt atacgataaa tatataaaaa gtaaagatga
      541 tatgttggta gtaacctgtc catgtacaca attaagagac tttgcaagtg aaaagtttaa
      601 ggataagggt aatgctatgt tttatacatg gaaagagttt gctgaacaag aaggtgtaaa
      661 atcacttgga aaaatagatg aatcaccaat tccattagga tatttcaaag atacttttga
      721 aaaagttctt gaagtatcaa gtgaagacga aatattaagt gaaatacaaa aaataagaaa
      781 tggttttgaa gataagtatg acatagtaga aatgttttat tgtaaagatg gttgtaataa
      841 tggagatggt ttgtaaatgg tcaataaatt taataataag tttttaaggc tagtaaagag
      901 tgttctgata ttttttataa ttcttttatt atggaaaatt acaaattatc ttggtatttg
      961 gagtgattat atattaccat cacccgaaaa ggtatatagt acatttttaa atatgataag
     1021 tgatggttca atatttatta atgtatatgc aagtatgaaa agagttttga taggttttgc
     1081 aataagtact gctataggtg tgcctttagg gatattcttt ggaatttata gtggagtata
     1141 cgaatacttt aaatctctaa taaatttttt aaggaataca cctccccttg ctttaatacc
     1201 aatgcttata ttatggtttg gtataggaga ggaatcaaaa attataatta tagttttagc
     1261 atcctttttc ccaatcttta caagcacatt gaaagggatt aaaaattgtg actcaaaact
     1321 gattgaagtg ggtagagtat ttgagttttc aaagcttcag ataatattta aaataataat
     1381 accaaatgcc attcttgata ttgctgttgg gttaaagtta gctcttggat atagctttag
     1441 agcaattatt ggggcagaac ttgtagcagc ttcatctggt cttggatact tgatatctga
     1501 tggaaaagaa atgtctagaa cagatgttgt tattgtaggt ataatcgtaa ttggactttt
     1561 aggaataatt acagattaca tattttcgat tattgtgaag aaggtaagta agggaaagat
     1621 ggtggaagca tatgaataat ttagtatgtg actctggata tataattaga aatttatata
     1681 aaagttataa agttgacaat gaagaacatg tagttcttag tgatatctct ttaaacatag
     1741 atagaaacca tataacagta attttaggtg aaagtggttg tggaaagact accttgctta
     1801 gagcaattgc atcacttgaa ggtataaata gtggtgaaat taggtttatt aatgataata
     1861 aagagtatag acctaatgtt ggttttgttt ttcaggagag cagattaatg ccttggttaa
     1921 acgtaagtga aaatatagct tttcataatc aaagaaaaaa tactatttta aacaaggtgc
     1981 ttagaaaatt taaacacaat aaaagaatta gcagtaaaat tataaatgat aattttagaa
     2041 gcttagaaac aaatgataat ttgaacaaat caaataaata taataggaaa agtaatattg
     2101 atgtagaaaa atatcttaag atgatgaact tgcagaaatt taaaaactct tatccaaatg
     2161 aattgtcagg aggtatggca cagagagtaa gcatagctag agctctttct ttcaatccag
     2221 atatgttgct tatggacgag cctttttcgg cattagatta ttttacaagg attgatatgc
     2281 aaaatgaagt tataagaata cataagtcaa ctaataaagg cgtgatattt gtaactcatg
     2341 atattgatga agcgttaaag ataggcaaga aaatagttgt tttcacagac gaaagaaaaa
     2401 taaaagaatt taatatagaa gatggttata atagagattt aacttcagat tactatatta
     2461 aattaaaaaa agatatttta atgacaatga aatcaaataa aggcatttaa agaaattaat
     2521 aatattttaa ggagtaaagt aaatgaaatt aaaaaagcta ttgataggta taacgatatt
     2581 aacacttgca acagcatctt tagctggatg tgctaaaaaa aataatggag aaaaactttc
     2641 agaaataaat ttaacttatg taaaatcacc attaaatgtt ccttctataa ttcagaaaca
     2701 ggatgattta tttggaaagg aatttaaaaa ggataatata aaagttaact tccacgaaat
     2761 tactacaggt ccagagcaaa cacaagcttt agctgctgga gaaatagact tcttacatgc
     2821 actaggtggt acatcagctt taatagctgc atctaatgga gtagagttaa agatattaaa
     2881 cacttatagc aggtcaccaa aaggatttat gattttgact aataataatt caataaaatc
     2941 agcagcagat ttagtaggta agaaggtggc aggcccaaaa gggactatat tacatcaggt
     3001 attgatttca gcacttgata aagaaggatt aagtatggat gatgtggaat ttgttaatat
     3061 gggtattcca gaggcatcag cagctctttc tgatggcagc gtagatgctg cattgattgc
     3121 tggtccagca gctcttaaag ctatgaagtc aggtagtaaa cttgtagcaa atggtgaagg
     3181 tttagtagat ggtataatag taactgctgt tagcacagac tttgcagaga aacatccaga
     3241 aatagttgaa agatttatga aggttgaaaa ggaaacttta gaatatgtaa ataataattt
     3301 tgatgaagct atggaaaaag tagctaaaga agttgattta agtcttgaag aaactaaaga
     3361 attatatgct tggtatgatt ttagtttaga tataacagat aaagacatat cttcgcttga
     3421 agatacacaa gacttcttaa taaaaaataa gttacaagag aaaaaagtga acataaaaga
     3481 attaatctat aatgctaagt agaaactatg ataaaataat actaagtaag aaatataata
     3541 aaagtt
//
LOCUS       DAELLV010000298         3501 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.298, whole genome shotgun sequence.
ACCESSION   DAELLV010000298 DAELLV010000000
VERSION     DAELLV010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.298"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            308..1021
                     /locus_tag="KPD58_003419"
     CDS             308..1021
                     /locus_tag="KPD58_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0267145.1"
                     /translation="MDTCFNKKILLVDDEKDIVDLIEEVLINDGFKNIIKAYNGLDAI
                     SLCKIACPDVVILDIMLPDIDGIEVCKKIREFSYCSILFLSSKNDDIDKILGLSSGGD
                     DYITKPFSPREIAFRVKAQLRRQQYQSIVPSDSEVIKIGDITIDIEGNRVYKDRNEIE
                     LTGREYHLLSYMAQNVNKIIGKERLYEQVWGVYSSICDNTIMVHIRHIREKIEDNPSN
                     PKILITVKGLGYKLVNRID"
     gene            complement(1293..2831)
                     /locus_tag="KPD58_003420"
     CDS             complement(1293..2831)
                     /locus_tag="KPD58_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG0267146.1"
                     /translation="MSLLKVTNLSQCFMDKSLYEKANFDLFKGEHIGVVGQNGTGKST
                     LIKILLGEVVPDSGEIKWQPNINIGHLDQYAEINRDTTISLYLHTAFEELYKIEKEMN
                     LLYQKSAISENEQYLIKASDYQEQLIASNFYSIDNEINKIANGLGLDSIGMNRVVREL
                     SGGQRAKVILAKLLLSNHDVLLLDEPTNFLDKEHVEWLSNYLNTFNGAFIVVSHDFDF
                     LEKISTGILDIEFGMIKKYHGKYSEFLKQKSHLREDYIRRYQAQQKKIEKEETFIRKN
                     KAGVNSKIARGRQKQLDKIERIAPPSFTGKPNIQFSEIEISAQNALTITNLEVGYYYS
                     LLPKLNFSVDGGQKIVITGFNGIGKSTLLKTLVKDIPRISGDFQFSEQVKIGYYEQDL
                     KWENPDKTPLQIVADKYPKLNTKEIRRHLARCGVKEEHVSRSVSTLSGGEQSKVKLCC
                     MMLSPCNFLILDEPTNHFDAETKDALQNALKQFRGSIILVSHEEKFYKGWIDKVFNIE
                     KQLV"
BASE COUNT         1217 a          466 c          490 g         1328 t
ORIGIN      
        1 tgaacttaaa gtttgattag aaaaagtcaa ttttaagaca actcctttta ttttactatt
       61 gtattaatct gtatctgtaa gtgtattagt ctaatattat aaactttgga tgaaattaat
      121 tttctatagg ttttaatgta tcatacactt tattttaaag aaacttatct taattttcat
      181 agtgattgaa tttgtctatt agagatgtta ctattagtaa tgtaaaagca agagtagtga
      241 atataaaaaa caagatagtg atactgcaag attagaatat aataaatttt tatggagaga
      301 tttcaatatg gatacatgtt ttaataaaaa aatattactt gttgatgatg aaaaggatat
      361 agtagattta attgaagaag tactcataaa tgatggtttt aaaaacatta ttaaagctta
      421 taatggtcta gatgctattt cgctgtgtaa gatagcatgt ccagatgttg tcattcttga
      481 tataatgtta ccagatatag atggtataga agtgtgtaaa aaaattcgag agttttcata
      541 ctgttctatt ctttttttat catcaaaaaa tgatgatatt gacaaaatac taggtcttag
      601 tagtggggga gatgactata taacaaagcc attcagtcca agagaaattg cttttcgagt
      661 taaagcgcaa ttacgtagac aacagtatca aagcattgta ccatcagata gtgaagttat
      721 taaaatagga gatattacta ttgatataga aggaaaccgt gtatataaag atagaaatga
      781 gattgagtta actggaaggg agtatcatct tttaagctac atggctcaaa atgtaaataa
      841 aattatagga aaagaaagac tctatgaaca agtatgggga gtatatagta gtatttgtga
      901 caatacaatt atggtgcata tacgacatat aagagaaaaa attgaagata acccctcaaa
      961 tcctaaaata cttataacag ttaaaggact aggatataaa cttgtgaata gaatcgatta
     1021 gaatagattt taatattaat gaagatatct caagttgaat ttttagttaa tgaggcattc
     1081 ttttatgggt gaaatcaaat gtattttcgt tatctcaaca atgaagaaga ggtctatctc
     1141 tattttgaga tagcctcatt aagtttatat cctggtaatg tactttatgt tctaataaaa
     1201 tttttttagt ttatttgcag tgaaacatta actagaacat taaaatttgc attgaaatac
     1261 caattaaata atattgtata ttgtgtataa acttatacta attgtttttc tatattaaat
     1321 actttatcaa tccaaccttt ataaaatttt tcttcatggg aaactaggat tatacttcct
     1381 ctaaactgtt tcaaagcatt ttgtaaagca tcctttgtct ctgcatcaaa gtgattggtt
     1441 ggttcatcca gaataagaaa attacaagga gataacatca tacaacataa tttaacctta
     1501 gattgttcac caccacttaa agtagataca ctccttgaaa catgctcttc cttcacacca
     1561 catctggcta gatgacgtct aatctctttt gtatttaatt ttggatattt atctgctact
     1621 atttgaagtg gggttttatc aggattttcc cattttaaat cttgctcata ataaccaatt
     1681 ttaacttgtt ctgaaaactg gaaatctcca gatatacgag gaatatcttt gactaatgtc
     1741 tttaacaagg tagatttacc gattccatta aaaccagtta taactatttt ttgacctcca
     1801 tcaacagaga aatttaattt tggaagtaaa gaatagtagt atccaacttc aagatttgtt
     1861 atagtaagag cattttgagc cgatatttca atttctgaaa attgaatatt tggttttcct
     1921 gtaaaactag gaggagctat tctttctatt ttatccaatt gtttttgtct tcctcttgca
     1981 atttttgaat ttactccagc tttattttta cgtataaatg tttcttcttt ttctattttc
     2041 ttttgttgtg cttgatacct tctaatataa tcttctctta aatgtgattt ttgtttaagg
     2101 aactctgaat attttccatg atatttctta atcatgccaa attctatatc aagaatacct
     2161 gtagaaattt tttctaaaaa gtcaaaatcg tgggaaacta caataaatgc cccattaaat
     2221 gtatttaaat aatttgagag ccattcaaca tgctctttat ctaaaaagtt tgttggttcg
     2281 tctaataata gaacatcatg gttagaaagt agtaattttg ctaaaattac ctttgccctt
     2341 tgaccaccac taagctctct aacaacacga ttcattccta ttgaatctaa tccaagacca
     2401 tttgctattt tatttatttc attatctata gaatagaaat tacttgctat tagttgttcc
     2461 tgataatctg atgctttgat tagatattgc tcattttcag atatagcact tttttgatag
     2521 agtaaattca tttccttttc tattttataa agttcttcaa atgcagtgtg taaatagagt
     2581 gatattgttg tatctctatt gatttctgca tattggtcta aatggcctat attgatatta
     2641 ggttgccact tgatttctcc tgaatctgga acaacttctc ccaataaaat tttaattaat
     2701 gtacttttac ctgtaccatt ttggccaact acacctatat gttcaccttt gaataaatca
     2761 aagttagctt tctcatacag tgatttatcc ataaaacatt gtgataaatt agttactttt
     2821 aataaactca taattcaaac tccttttctt ttattaaaaa atttatggaa ataaaaaaag
     2881 cagaggaatg gcattgtaaa atgccgacct ctgcttttgg aaaaacaaaa ggtcatagac
     2941 aagcattgtt gtctatgacc agaaaatcta tattatctat cggcaaatag gtaattttaa
     3001 ataagcatag ccaaaaaact atactaaacc tatatttgcc gatgaagata gaaagcttta
     3061 agctttcaga ttaacctcta cacaacgctt catcgtatac atttgtgtag agttaagttt
     3121 tctaacaagt ttaactaatt ctatataaat cataattatt cacatcctaa tcttagtatt
     3181 aattctagtt tatctgtatt taaattgtac ttttgtactt tgtggattat cttatttatt
     3241 taaaatatgt tttattaatt atagcttaag ttatgttatc ataatactta gttatagtca
     3301 ataagaaagt tatatttact attattatat taatttagta aatctatcat tacaaagtta
     3361 tttgaagact cgctatcaat aaaattttat agtctataag aaaattaaga ttatgagttt
     3421 tataattttg atatagggat actattttca taaatattac aacatgtaga agtatatgtc
     3481 tattattgtt gaattttcaa t
//
LOCUS       DAELLV010000299         3470 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.299, whole genome shotgun sequence.
ACCESSION   DAELLV010000299 DAELLV010000000
VERSION     DAELLV010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.299"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            802..1737
                     /locus_tag="KPD58_003421"
     CDS             802..1737
                     /locus_tag="KPD58_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBG0267147.1"
                     /translation="MYYIGVDIGGTGIQAGVVDNYGKIIFRSECKTVIEKGFEGILND
                     IKIMIYKLLEDNKLTMSDIKSIGFGVPGFINKEGLVTCVNLKWNKKAFNKELKRRFPD
                     VEIHGENDATVAALGEAKFGSMKGANVGVLYTLGTGVGGGIVINQKVFSGAHGLGSEI
                     GHQIIGENYFNCNCGNNGCVETFCSATAIIKYSQKLIEEGEKSRILDLAEGNLENVNA
                     KMVFDAYRENDLVAIKVINRFKEYLAKTFANTINSLDPEIISIGGGISKSSDIILDGI
                     EDLVRKFVLYKTEDIATITCATLGSDAGIIGAAFL"
     gene            1773..3146
                     /locus_tag="KPD58_003422"
     CDS             1773..3146
                     /locus_tag="KPD58_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0267148.1"
                     /translation="MGKTNARRTLFLIAIGSGTMLNPLNSSMISLALHSIQNDFHISF
                     TTVSWIVSAFYLASAIAQPVSGKIGDLIGRKVLFLSGLMLVILSASIVPLVQSFFILI
                     FIRVIQAIGTSTLYPSGVALIQNNIKERQSSALAVLTIFASTTAGLGPTLGGLLLDLG
                     GWHAIFLVNIPVVLVSICLGYFLFPKEVKEKKSIKETLKNVLSRLDIIGILLFSIAMI
                     FILLFLLSMKESFNLEQLIFGIILMCLFIWHELRTKLPFIDIKLFVSNPKLSKVYLQF
                     IILNFFNYILFFGLPSYFQDALHYSAKSTGLFMLFMSGIGIFISPLTGKWIDKSGTRF
                     PLVTSSIFMFISAVLLSVVFIHIPVIGKGIILSLAGISYGVGNVALQSSMFEESPRDS
                     IGTASGLFQTSRYLGSILATVLLGMVFEVNITSEQFQILGYVMVVLGTISFLLNFILK
                     KELRKVE"
     gene            complement(3334..>3470)
                     /locus_tag="KPD58_003423"
     CDS             complement(3334..>3470)
                     /locus_tag="KPD58_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0267149.1"
                     /translation="FLHRNTLVYRLEKINEILGFDLKNFENAMIFYLSVKSYFLYKKI
                     "
BASE COUNT         1258 a          351 c          611 g         1250 t
ORIGIN      
        1 atataatgtg ctataatata atcaaataaa agaaaggggt gattccagtg ggaacggatg
       61 tggcactgcc aaatgatgaa gaaaacttta ttgtaatata caaaataaaa ttagatactt
      121 ttagtgctag attaagtact ggaaaaactc atgagctggg ataattattg taattaccta
      181 gactcaaact tttagtagta aaatagtaat attatataga caaggtatct aatttttaaa
      241 tcttttgtat gaattaaaat ttttataaag ggtatatctg attagaatgt aattagggaa
      301 acctagttgt gaattacttt tagtatatac atgtgtaaag aaatatgtat atggtattgg
      361 ttattgtgct agatgtgtat gtaaagaaat atgtgtctag ttgggtatat gtaaagaaat
      421 atacttataa tctatgaaaa atttttagta taaattgcta aaacattgag attgtttttt
      481 atagaaagtt agtttatacc aactaaaaat gtgcttaaga tttgtttgat gtttaattat
      541 aaataaggtt gtagataaaa aaataagtaa agacaaaggt ttaaaatact tgctttaaat
      601 atagtttgtt ttgaacactt gccaaattaa aataattaat aaaatagaca tcttctcata
      661 gtaaaatata ctgtgagagg atgtttttgc tttgttcata aacttttatg aaaaatttgc
      721 atagtgtcac attttaatat actatatata taataaaata tagataaaat taaatgattc
      781 aaaagagaaa ggtgaaaaga gatgtattat ataggtgtag acataggagg aacaggtata
      841 caagcaggag tagttgataa ctatgggaaa ataattttta ggtcagagtg taaaacagta
      901 attgaaaaag gatttgaagg aatacttaat gacataaaaa taatgattta taagttgttg
      961 gaagataata aacttactat gagtgacata aaatctattg gatttggtgt tccaggtttt
     1021 ataaataaag aaggattagt aacttgtgta aatttaaaat ggaataaaaa agcttttaat
     1081 aaagagttaa aaagaagatt tccagatgtt gaaattcatg gagaaaatga tgcaacagta
     1141 gcagcacttg gagaggcaaa gtttggaagt atgaaaggtg ctaatgttgg agtattatat
     1201 actttaggaa ctggtgtagg tggaggaata gtaattaatc aaaaagtctt ttctggagca
     1261 catggattgg gttcagaaat aggtcatcaa attataggag aaaactattt taattgtaat
     1321 tgtggaaata atggttgtgt agaaactttt tgttcagcaa ctgcaattat aaagtattct
     1381 caaaaactga tagaagaagg agaaaagagt aggattttag atttggcaga gggaaattta
     1441 gaaaatgtaa atgcaaagat ggtgtttgat gcatatagag aaaatgattt agtggcaatt
     1501 aaagtaataa atagatttaa agagtattta gcaaaaactt ttgcaaacac aataaattct
     1561 ttagacccag aaattatatc tataggagga ggcatttcta aatctagcga tataatactt
     1621 gatggaatag aagatttggt aagaaaattt gtattataca aaacagaaga tatagctaca
     1681 attacatgtg caactttagg ttcagacgca ggaattatag gagcagcttt tttataatgt
     1741 aaatgaactg cttattggag gcgtgatgat atatgggtaa aacaaatgct agaagaacgc
     1801 tttttttgat agcaataggt tcaggaacta tgctgaatcc tcttaattct tctatgattt
     1861 cacttgcttt acatagtata caaaatgatt ttcatattag ttttacaact gtatcttgga
     1921 ttgtttcagc attttaccta gctagtgcta ttgcacaacc agtttcaggt aagataggag
     1981 atttaatagg tagaaaggtc ttatttttga gtggtttgat gcttgtaatt ttatcagcat
     2041 ccatagtacc acttgttcaa tcattcttta tattgatttt tataagagta attcaagcaa
     2101 ttggaacaag taccctatat ccatctggag ttgctcttat acaaaataat ataaaagaaa
     2161 gacaatcttc tgcattagca gttctaacca tttttgcttc aactacagca ggattaggtc
     2221 cgacacttgg aggattattg ttggacttag gaggatggca tgctatattt cttgtaaata
     2281 tacctgttgt tttggtttct atatgcttag ggtatttttt attcccaaaa gaagtaaaag
     2341 aaaagaaatc tataaaagaa actttaaaaa atgttttgtc aagattagat attattggca
     2401 tacttctatt ttcaattgca atgattttta tattactatt tttgctatct atgaaggagt
     2461 cttttaactt agaacaactt atatttggta taatactgat gtgtcttttt atttggcatg
     2521 aattgagaac aaaattgcca tttatagata ttaagctttt tgtaagtaat cctaaattgt
     2581 caaaagttta cttgcaattt attatattaa atttctttaa ttatattttg ttttttggtc
     2641 ttcctagtta ttttcaagat gccttacatt atagtgcaaa gtcgactgga ttatttatgc
     2701 tatttatgtc tggtataggt atatttatat ctccattaac tggaaaatgg atagataaat
     2761 ctggaactag atttcctctt gttacatcat caatctttat gtttattagt gcagtacttc
     2821 tttcagtagt ttttatccat atacctgtaa taggaaaggg tattatatta tcattagcag
     2881 gtattagtta tggagttggt aatgtagccc tgcaatcctc tatgtttgaa gaaagtcctc
     2941 gtgacagtat agggacagct tctggtttgt ttcaaactag tcgttattta ggttctattt
     3001 tagcaactgt tttacttgga atggtatttg aagtaaatat aacatcagaa caatttcaga
     3061 tattaggtta tgttatggtg gtcttaggta caataagttt tttattaaat tttatattaa
     3121 agaaagaatt aagaaaagta gaatagtgga tttatggatt taataagtct aataactgat
     3181 aagtttaaaa atattgtttg taaaacataa ctggtttggt atttattaat tctaagtttt
     3241 gaagcataga gtactcgata agtatacaat attttgaatt aaaattaagc agaaatttga
     3301 actagtcttt caaatctctg cttaatttag tagttaaatt tttttataga gaaaataaga
     3361 tttgacactt agataaaaaa tcattgcatt ttcaaaattc tttaaatcaa acccaagtat
     3421 ctcattaatc ttttccaatc tatatactag cgtgtttcta tgtaaaaaca
//
LOCUS       DAELLV010000300         3431 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.300, whole genome shotgun sequence.
ACCESSION   DAELLV010000300 DAELLV010000000
VERSION     DAELLV010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.300"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            279..926
                     /locus_tag="KPD58_003424"
     CDS             279..926
                     /locus_tag="KPD58_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HBG0267150.1"
                     /translation="MIRQTPLEKRYDKLGPHIVTALKKRYFDAYYCKTRNEALQQILD
                     LIPQNHVVSWGGSETLKEMGVQTAVREKGFKVIDRDLAKTPEERTEIMRQALLCDTFL
                     MSSNAISEDGQLFNIDGNGNRVAAMIFGPKNVIVVAGMNKIVKNIDDAIQRTRTIASP
                     AVVQRFQDVNTPCYINGSCIDCTSPESICAYMVTTRISRPAKKIKVILVGENLGL"
     gene            complement(1093..1662)
                     /locus_tag="KPD58_003425"
     CDS             complement(1093..1662)
                     /locus_tag="KPD58_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG0267151.1"
                     /translation="MKKFIICIIMFAISVLVLAGCTNDNKIKNEVDSMFNSIKTQDAD
                     TVDKYFPKSGLGNLIYEDKEGFKMYSKNMTWEISDIKNNKDKVTVDLKINNTDMVSIL
                     KKNPPKLGEMMPNPTMKDIDSAKKKEFNITLELVKSGDNYVCKVNQESAMKLLNVITG
                     GGIDYLADYNKDYYKQLDEEQKNYENKNS"
     gene            complement(1667..2566)
                     /locus_tag="KPD58_003426"
     CDS             complement(1667..2566)
                     /locus_tag="KPD58_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG0267152.1"
                     /translation="MKLLSKVNNNIRLITQVAFTALTNGYVNGFLEGTIYGGESKKLC
                     VPGLNCYSCPGALGACPIGSLQAVLSSREYKFSFYVVGFLMAFGAFFGRFVCGWLCPF
                     GLVQDLLHKVPFPVKIKKVYGDKYLKYLKYIVLIMFVIILPVAIVNVAGGGNPWFCKW
                     ICPSGTLLGGIPLILGNENLRESIGFLFSWKLSILLLILLMSIITYRPFCKYICPLGA
                     IYGFFNPISIYRFKIDKDKCTNCTACQTKCKLDIPIYKDVNSPDCIRCGECIKVCPQN
                     AITTTFSKDKKENNICAKKYRGI"
     gene            complement(2577..2726)
                     /locus_tag="KPD58_003427"
     CDS             complement(2577..2726)
                     /locus_tag="KPD58_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0267153.1"
                     /translation="MSNFIKDNKKSISIIAISIIFIALGTYRGETNTVLTKAIMICLE
                     CIGIG"
     gene            complement(2719..3303)
                     /locus_tag="KPD58_003428"
     CDS             complement(2719..3303)
                     /locus_tag="KPD58_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HBG0267154.1"
                     /translation="MKRFFIVVLGCICVLGMISGCSKKESKPVEKNKNNSEWFSNLDT
                     EDVDGNKVTKEIFSNKELTLINVWTTWCGPCVGEMPELEALSKEYESNNSNVAIKGLV
                     VEVDKTDMRTGLSNEEKNLVKDIMKKSDATYQQLTVSEELKKTDFKRVMEFPTTYFVD
                     KKGNFVGEKVVGANSKEDWKKIIDERLKMVKANE"
BASE COUNT         1213 a          572 c          380 g         1266 t
ORIGIN      
        1 taaaataata atcatctata acccacaact ttattttata ttaaataaaa agtattagtg
       61 attatgtttt ataggtattt ctttattcag ttatttatta aaaactaata gtaaatctta
      121 tactaatcgc atatttatgt tataactata ttgtaactat tttcgtaatc ttaggaaaag
      181 gttacttata aaattaacct tatacaataa tatatcgtat aatcttaatt agcataatag
      241 ttacaatatt aaaatttaaa tatgggagtg attttagtat gataagacaa acaccattag
      301 agaaacgata tgacaagcta ggtcctcata ttgtaacagc actcaaaaaa cgttattttg
      361 atgcttatta ctgtaaaact agaaatgaag ctttgcaaca aattttagat ttaattcctc
      421 aaaatcatgt agtatcatgg ggaggatctg aaacacttaa agaaatgggc gtacaaactg
      481 ctgttagaga gaaaggattt aaagttattg atagagattt agcaaaaact ccagaagaaa
      541 gaactgaaat tatgcgtcaa gcacttctat gcgatacttt tttgatgagt agcaatgcaa
      601 ttagtgaaga tggacaactc tttaatattg atggaaatgg caatcgtgta gctgcaatga
      661 tatttggtcc aaaaaatgtt atagttgtag ctggtatgaa taaaattgta aaaaacattg
      721 atgatgcaat acaaagaact agaacaattg catctccagc agttgttcag cgttttcaag
      781 atgtcaatac accatgctat attaatggaa gttgtataga ctgcacaagt cctgaaagca
      841 tttgtgcata catggttact acaagaatca gtcgtccagc aaaaaaaata aaggtaatat
      901 tggtaggaga aaatcttggt ctataaaata tacatacatg taaatagctc tataaataat
      961 aaaatcatat gaaataactt atttctaaaa gtggtagaat aaaagcttct acaagacttt
     1021 cattctacca cttttatatt cttttaattt gtgtttctat attattaatt gctccgattt
     1081 tcatatatat tactaactat ttttattttc ataatttttt tgttcttcat caagctgttt
     1141 ataataatct ttattatagt cagctagata atctatacca cctcctgtaa tcacatttaa
     1201 taatttcatt gcagattctt gattaacttt acaaacataa ttatcaccag atttaacaag
     1261 ctctaatgta atattaaatt cttttttctt tgcagaatca atatctttca tagttggatt
     1321 tggcatcatt tctcctagtt taggaggatt cttttttaaa atagaaacca tatcagtatt
     1381 gttaattttt aaatctacag tcactttatc cttattattc tttatatctg aaatttccca
     1441 agtcatattt ttagaataca ttttaaatcc ttctttatct tcatatataa gattacctaa
     1501 ccctgatttt gggaaatact tatcaacagt atctgcatct tgtgttttta ttgaattaaa
     1561 catactatcc acttcatttt ttatcttatt atcattagta caaccagcta acactagtac
     1621 tgatattgca aacattataa tacatataat aaatttcttc attaaattat atccccctat
     1681 acttttttgc acaaatatta ttctcttttt tatcttttga aaaagttgta gtaattgcat
     1741 tttgtggaca aacttttata cattcaccac acctaataca gtctggacta tttacatctt
     1801 tatatattgg tatatcaagc ttacattttg tttgacatgc tgtacaattt gtacatttat
     1861 ctttatcaat tttaaatcta tatattgaaa taggattaaa aaaaccatag attgctccta
     1921 aagggcatat atacttgcaa aatggcctat aagtaatgat tgacatcaat aatatcaata
     1981 gcaaaatact taacttccag ctaaataaaa atcctattga ttctcttaaa ttctcatttc
     2041 ctaaaataag tggtattcct cccaaaagtg ttcctgaagg gcaaatccat ttacaaaacc
     2101 aaggatttcc accccctgct acatttacaa tagcaactgg caaaataatt acaaacatta
     2161 ttaagactat atactttaga tattttagat atttatcacc ataaactttt tttatcttta
     2221 ctggaaaggg tactttatgt agtaaatctt gtacaagtcc aaagggacaa agccaaccac
     2281 aaacaaatct gccaaaaaaa gcaccaaatg ccattaaaaa accaacaaca taaaatgaga
     2341 atttatactc tctgcttgat aaaactgctt gtaatgaacc tattgggcat gctccaagtg
     2401 cacctggaca tgaataacaa ttcaatccag gtacacataa ttttttactt tctcctccat
     2461 atatagttcc ttctaaaaat ccatttacat atccatttgt taatgctgta aatgcaactt
     2521 gagtaataag tcttatatta ttatttactt tagataataa cttcattcct cttctcctaa
     2581 ccaataccaa tacactcaag acaaatcata attgctttag ttaatactgt atttgtttct
     2641 ccacgatatg tacctaatgc aataaatata atactaattg caataataga aatgcttttt
     2701 ttattatctt taataaaatt actcattggc ttttaccatt tttagtcttt catctataat
     2761 ttttttccaa tcttccttac tatttgcacc tacaaccttc tcacccacaa agtttccttt
     2821 cttgtctaca aaataagttg ttggaaactc cataactctt ttaaaatcag tttttttaag
     2881 ttcttcagat acagtcaatt gttggtaagt tgcatctgac tttttcataa tgtctttaac
     2941 taaatttttc tcttcatttg aaagaccagt tctcatatca gttttatcaa cttctacaac
     3001 taatcccttt atagctacat tggaattatt actctcatat tcttttgata atgcttcaag
     3061 ctctggcatt tcacccacac atggtccaca ccaagtagtc cagacattaa taagagtaag
     3121 ttctttattt gaaaatatct cttttgttac cttatttcca tcaacatcct ccgtatctaa
     3181 attactaaac cattcactat tattcttgtt tttttctact ggcttagatt cttttttgct
     3241 acatccagaa atcataccta aaacacaaat acatcctaaa actactatga aaaacctttt
     3301 catatctcac actcctagaa tttatatagt atataaaatc gctgcttgaa ataaaaataa
     3361 cttatcttct atattaaagt aattatattt aataaataag agctaattta aaaaaagata
     3421 tacagttaaa a
//
LOCUS       DAELLV010000301         3422 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.301, whole genome shotgun sequence.
ACCESSION   DAELLV010000301 DAELLV010000000
VERSION     DAELLV010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.301"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..496)
                     /locus_tag="KPD58_003429"
     CDS             complement(<1..496)
                     /locus_tag="KPD58_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HBG0267155.1"
                     /translation="MESKGTLSKNKVYILANGLFLVIALLNINLYTVSINMYRIILLW
                     IFGYSIVYISSFKTDEFINILKVGCLVLVFVSILGVVNLKIQKFQIVLLNLSIINMST
                     FIYLTISKVKKLRISPILIFYSAFVILDYFIRNIFSDINWNMIIILINICISMFNLLY
                     LLNKL"
     gene            complement(644..1435)
                     /locus_tag="KPD58_003430"
     CDS             complement(644..1435)
                     /locus_tag="KPD58_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG0267156.1"
                     /translation="MKLKKLLSVALVSAIAISAVGCSNKEDKKILVGASSNPHAKILE
                     VAKPLLKEKGYDLEVKIFDDYVLPNTALDEGSLDANFFQHIPFLEETVKEKGYKLTYT
                     SKVHIEPMGFYSEKVKSLDELKDGAVIAVPNDATNGARALKLLAKNKLIEVKDGELIT
                     KKDITKNPKNIVIKEMNAEQLPTVLKDVDGAVINSNYALTANLNPTKDAIVIESSDSP
                     YVNIIACRENNKDSDKIKALSEAMNSKEVKKFIQDEYKGSIVPAF"
     gene            complement(1471..2127)
                     /locus_tag="KPD58_003431"
     CDS             complement(1471..2127)
                     /locus_tag="KPD58_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0267157.1"
                     /translation="MNSLIDFLTTLFPNALLQTLYMVIVPTIVATILGFILAIILVVT
                     KPDGLKPNSTINSALGFIVNIFRSFPFMILIVAMIPITRLIVGTSIGETAAIVPITIG
                     AAPFIARIIESSLNEVDKGLIEAAKSFGATKRQIVFKVMIKEAMPSIVSGITLSIISI
                     LGYTAMAGAVGAGGLGNIALIYGYQRFDTAVMVYTVIALIILVQIIQGVGNLAYKKLK
                     "
     gene            complement(2120..3085)
                     /locus_tag="KPD58_003432"
     CDS             complement(2120..3085)
                     /locus_tag="KPD58_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HBG0267158.1"
                     /translation="MISIKNVNKYYGKIQVLKDVSIEIESGEIFGIIGHSGAGKSTLL
                     RCINGLEEYQEGSVLVSDKEVKSLNEKQMRDLRKELGMIFQHFSLLERKTVFDNVALP
                     LECFGYSKAEIKKRVLELLEVVGISEKKNDKPRNLSGGQKQRVAIARALALNPQVLLC
                     DEATSALDPNTTKSILSLLEDINKKLGITIIVVTHQMEVIKQICGRVAIMENGEVLEV
                     GDTEEIFLRNTKGLRKLIGEESIILPKGTNIKILFPKDISNEAIITTMARELNIDVSI
                     IFGKLEQFKDDILGSLIINISDKSGEQVKQYLTSKGIRWEEMINE"
     regulatory      complement(3191..3297)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
BASE COUNT         1073 a          596 c          349 g         1404 t
ORIGIN      
        1 caagtttatt caacaaatat agtaaattaa acatagatat acaaatatta attaatataa
       61 taatcatatt ccagttaata tcactaaaaa tatttcttat aaagtaatct aatatcacaa
      121 aagcactata aaaaattagt attgggctta ttcttagttt ttttacttta gaaatggtta
      181 agtatataaa agtagacata ttaattatgc ttaagttaag caatacaatt tggaattttt
      241 gaatttttaa atttactact cctaaaatac ttacaaaaac taatacaaga caccctactt
      301 tcaatatatt tataaattca tctgttttaa agctacttat ataaactata gaatatccaa
      361 atatccaaag taaaattatt ctatacatat ttatagaaac tgtatacaaa tttatattta
      421 aaagtgcaat aaccaaaaat aacccattag ctagtatata gaccttattc ttacttaaag
      481 ttcctttaga ttccatattc cccaccactt cttcattttt atatagtttt acctataaat
      541 atgttaccac aattcttcca aaaaatttgt cactttctag ttataaaaaa aaatgagtaa
      601 atatttttaa ttttactcat tttgtagatt ttgtatattc agtttaaaaa gctggaacta
      661 tacttccttt atattcatct tgtataaatt ttttaacttc tttactattc atagcctctg
      721 ataaagcctt aatcttatca ctatctttat tattttctct acaagctatt atatttacat
      781 atggagaatc acttgattct attactattg catccttagt aggatttaag ttagctgtta
      841 aagcataatt agaatttatt actgctccgt caacatcttt taatactgtt ggtaattgct
      901 ctgcattcat ttcttttatt actatatttt taggattttt agttatatct tttttagtta
      961 taagctctcc atcttttact tctatcaatt tattttttgc taataattta agtgctctag
     1021 ctccattagt tgcatcattt ggcacagcta taacagctcc atcttttaat tcatctaatg
     1081 acttaacttt ttctgaatag aatcccattg gctctatatg aactttagat gtataagtaa
     1141 gtttatatcc tttctccttc acagtttcct ctaaaaatgg aatatgttga aagaagttag
     1201 catccaaaga cccttcatct aaagctgtat ttggaagtac ataatcatca aatattttta
     1261 cttctaaatc atatcctttt tcttttaaca aaggttttgc aacttccaat atctttgcat
     1321 gtggatttga tgaagctcct actagtattt ttttatcctc tttgtttgaa caacctactg
     1381 ctgatattgc tattgctgac accaatgcta ctgataataa tttctttaat ttcattttta
     1441 ctccccctct ttaaaatgtt tataattaat ttattttagt ttcttataag ccagatttcc
     1501 tactccctgg attatctgaa ctaatattat aagtgctatt acagtatata ccattactgc
     1561 tgtatcaaat ctttgatatc cataaataag tgctatatta cctaatcctc cagctcctac
     1621 tgcacctgcc atagctgtat aaccaagtat agatattatt gataatgtaa ttccagatac
     1681 tattgaaggc atagcttcct ttatcataac tttaaatact atttgtcttt ttgtagctcc
     1741 aaaagatttt gctgcttcta tcaatccttt atcaacttca tttaatgagc tttctataat
     1801 tctagcgata aacggtgctg ccccaatagt tattggcact attgcagcag tttcccctat
     1861 acttgttcca acgattaatc tagttattgg aatcattgca actataagta tcataaatgg
     1921 gaaacttcta aatatattta ctataaatcc taatgcacta tttattgtgc tatttggttt
     1981 taatccatct ggttttgtaa ctactaatat gattgctaat ataaatccta atattgttgc
     2041 cactatagtt ggtactataa ccatatacaa agtttgtaac aatgcatttg gaaatagtgt
     2101 tgttaaaaag tcaattaaac tattcattta tcatttcctc ccatcttatg cccttactag
     2161 ttagatattg ttttacttgc tctccacttt tatctgaaat gtttataata agtgaaccta
     2221 aaatatcatc tttaaattgt tctaatttcc caaatattat agacacatct atatttagtt
     2281 ctcttgccat agtcgtaata atagcttcat tagaaatatc tttagggaat agtatcttta
     2341 tatttgttcc ttttggcaat attatacttt cttcacctat taattttcta agtcctttag
     2401 tatttcttaa gaatatttct tctgtatctc ctacctctaa tacttctcca ttttccatta
     2461 ttgctactct tccacaaatc tgctttataa cttccatttg atgagttaca actattattg
     2521 ttattcccaa ttttttattt atatcttcaa gcaatgaaag aattgacttt gtagtatttg
     2581 ggtctaatgc tgaagtcgct tcatcacaaa gaagtacttg tggatttagt gctaaagctc
     2641 tagcaattgc aaccctttgt ttttgtccgc cacttagatt tctgggttta tcatttttct
     2701 tttcagatat tccaactact tctaataact ctaaaactct ttttttaatt tctgcctttg
     2761 agtatccaaa acattctaat ggcaatgcta cattatcaaa tacagttttt ctttcaagca
     2821 atgaaaagtg ttgaaaaatc attcctaact cttttcttaa atctctcatt tgcttttcat
     2881 tcaatgactt tacttcttta tcagaaacaa gtacactgcc ttcttggtat tcttctaatc
     2941 cgtttataca tctcagtagt gttgatttcc ctgcaccact atgacctatt attccaaaaa
     3001 tttctccaga ttctatttca atacttacat ctttaagcac ttgaatcttt ccataatact
     3061 tattaacatt ttttatgcta atcataaaat ttcctcccat atattctgct cagtttttat
     3121 gtttatcctc tcttatgtat tacaacaaaa taaaagcccg agtataaact caggcttaga
     3181 aagctattta tactcatcta tcaactatat tagttgcagg atttagcacc aatgtatata
     3241 aaatatactg gttgccgggt ttcaaagggc cagtccctcc accactcttg ataagacgtt
     3301 atttaattta attctattat catttgttgc tatgttatat atgatatatt cattaatagt
     3361 atttgtcaat aaatttattt aaatttttta ttttttatta tcgttaatta actattttta
     3421 at
//
LOCUS       DAELLV010000302         3397 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.302, whole genome shotgun sequence.
ACCESSION   DAELLV010000302 DAELLV010000000
VERSION     DAELLV010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.302"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(64..2145)
                     /locus_tag="KPD58_003433"
     CDS             complement(64..2145)
                     /locus_tag="KPD58_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HBG0267159.1"
                     /translation="MKKFIISIISLVLFFSNISLIYKVNADETKVKNYKFERDITIDG
                     VIGSNSTFFEVNKNWDIEEVLLHLNFSKSQILNGDVSSLTVLINNVPIKSIKLNAKTN
                     YKNTLEVLVPKDYIIQGYNEIKIKTYKTISDKICQDDSNTGNWMVIHKESYISIRYKQ
                     KKVENSINEYPYPYAEIENNHKLDTTIVVPDNMTRGETTAVFNLASAFGKITKNDDLK
                     LDVKLYSEMKNWSDDNIIYIGKPENTAEEILDILSIKEQTLLSSNCIIKQVDSPYNKN
                     KKMMVVIGSNEDDLIKASNLLIENRLSNQVLSSSVLVNKETNIKINREQKLNLGHLTL
                     KDLGYSDFLLEGAFNQQALFDVKIPTGKVLDDGSKIILNLRYSDNLDFEKSLVTVSIN
                     DVIVGSKKLDRSHSNNDKLELKIPKDIDNKNYYQVKLTFNLSIKNSNCVTRESNNPWA
                     YVSNNSYLALSTKENETLSFENYPYPFVRDDEFNDLTVIMPDYSGSQAMTWMFRLGVT
                     LGANINSHNGNINVIRGKEFSDKYKDTNIVVFGVPHNNSVIKMLNNNLNIKFDKNYSN
                     FISNDKISFIDDYGKNISTIQLIKSPYNNQKNIMVISSMNEKNLYLGMDYLLNKSKVN
                     DLKGDTLIIDEYGEVEDLAYNLKSKKEVKDSSWNMSINKTTKVFLMISFITIIIVMIL
                     SMLYIKKYKRR"
     gene            complement(2168..3397)
                     /locus_tag="KPD58_003434"
     CDS             complement(2168..3397)
                     /locus_tag="KPD58_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003495444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG0267160.1"
                     /translation="MFVFSLFSIWSLLLINIILAMGGYIFYFKNFDKEIKEIDEYPMI
                     SILVPAHNEAKVIGRTVESLLLLNYPKSKMELIVINDNSSDNSKEILENIKDRYNNYN
                     FTIINTDSLTGGKGKSNALNIGYTISKGDFIAVYDADNTPDKNALRYLVQTIVMNDEL
                     GAVIGKFRTRNKNKNLLTKFINIETLSFQWMSQAGRWQLFNLCTIPGTNFILRRSIIE
                     EIGGWDSKAIAEDTEISFRIYKLGYKIKLVPQSITWEQEPETVKVWIKQRTRWAKGNI
                     YVLMKYIKNIFKQGRNKIVFDIAYFFSVYFLFLTSVIISDILFVLSISKLVEISIPIN
                     FFLIWILSYLLFIIEVSISLTIEKGEATIENIFIVAIMYFTYSQLWLFVAIKGMIEYL
                     KDIIFKREVKWYKTERF"
BASE COUNT         1141 a          538 c          307 g         1411 t
ORIGIN      
        1 tttaaattca ttcatatatt tcctcaaatt atatacctct tttaaaataa taaatttatc
       61 atattatctt cttttatatt tttttatata tagcattgat aatatcatga ctataattat
      121 agtaataaaa gaaatcatta aaaatacttt agttgtctta tttatactca tattccaact
      181 tgaatctttt acttctttct tagattttaa attataagct aaatcttcta cctctccgta
      241 ttcatcaatt atcaatgtat ctccttttaa atcattaact ttacttttat tcaacaaata
      301 atccattcct aaatataagt ttttttcatt cattgaacta attaccatga tatttttttg
      361 attattatat ggtgatttta ttaattgaat agtagaaata ttttttccat aatcatcaat
      421 aaagcttatc ttatcatttg atataaaatt ggagtaattt ttatcgaact ttatatttaa
      481 attattattt aacatcttta taacagaatt attatgtggc actccaaaaa ctacaatatt
      541 agtatcctta tacttgtcac taaattcttt tcctctaatt acatttatat ttccattatg
      601 tgaatttata ttcgcaccta aagtaacacc taatctaaac atccaggtca tagcttgtga
      661 ccctgaataa tctggcatta tcacagttaa atcattaaat tcatcatctc taacaaatgg
      721 atacggataa ttttcaaaag ataaggtttc attttctttc gtagataacg ccaaataaga
      781 attatttgat acatatgccc aaggattatt actctctcta gtaacacaat tcgaattttt
      841 tatactcaaa ttaaatgtca atttaacttg ataatagttt ttattatcta tatctttagg
      901 tatttttaat tctaacttat cattattaga atgtgaccta tccaattttt tacttcccac
      961 aataacatca tttatagaca ctgtaactaa agatttttca aaatctaaat tgtcagaata
     1021 tcttaaattt aagataatct ttgaaccatc atctaatacc ttacccgttg gtattttgac
     1081 atcaaacaaa gcttgttgat taaatgctcc ttccaacaaa aagtcagaat atcctaaatc
     1141 ttttaatgtt aaatgaccta aatttaattt ttgttcccta ttaattttta tattagtctc
     1201 tttgtttaca agtactgatg atgataaaac ttggttagaa agtctatttt ctattaataa
     1261 atttgatgcc tttattaaat catcttcatt actaccaatt accaccatca tcttcttatt
     1321 cttattgtat ggtgaatcta cttgcttgat tatacagttt gaagataata gggtttgttc
     1381 ctttatactt aaaatatcca aaatttcttc tgctgtattt tcaggtttac caatatatat
     1441 aatattatca tctgaccaat ttttcatttc tgaatataat ttaacatcta actttaaatc
     1501 atcatttttt gttatttttc caaatgctga tgccaaatta aacactgctg tagtctctcc
     1561 tctagtcata ttatctggta ctactatagt tgtatctaat ttatgattat tttcaatttc
     1621 tgcatatgga tacggatatt catttattga gttttccacc tttttctgct tatatctaat
     1681 tgatatatat gattctttgt gaataaccat ccagttccca gtattggaat catcttggca
     1741 aattttatca gatattgttt tgtatgtttt tatttttatt tcattatatc cttgaattat
     1801 ataatctttt ggaactaata cttctaatgt atttttataa tttgtcttag catttaattt
     1861 aatagatttt attggtacat tatttatcaa tacagttaaa cttgaaacat ctccatttaa
     1921 tatttggctc ttactaaaat taagatgcaa taaaacttct tcaatatccc aatttttatt
     1981 tacttcaaaa aaagtactat tacttcctat aacaccatca atagttatat ctctttcaaa
     2041 cttataattt ttaactttag tctcatcagc atttactttg tatataagtg atatatttga
     2101 aaaaaacaat actaaagata taattgatat aataaatttt ttcatgttaa ttcctcctgt
     2161 acatgaatca aaatctttct gttttatacc atttaacctc tcttttaaaa attatatctt
     2221 ttagatactc tatcattcct tttatagcta caaacagcca tagttgagag tatgtaaaat
     2281 acattattgc cactatgaat atattttcaa ttgttgcttc acctttttct atagtaaggc
     2341 ttatactcac ttctataata aataaaagat aagatagtat ccatattaga aaaaagttaa
     2401 ttggaataga aatttctact agtttagata tacttaaaac aaataaaata tcggatataa
     2461 ttacagatgt taaaaataga aaatatacag aaaaaaagta agctatatca aacacaatct
     2521 tatttcttcc ctgtttaaat atatttttta tatatttcat taatacatat atattccctt
     2581 ttgcccatct agtacgttgc tttatccaca ccttaacagt ttctggctct tgttcccatg
     2641 ttatagattg tggtacaagc tttatcttgt atcccaattt atatatccta aaacttattt
     2701 ctgtatcttc tgcaattgct ttactatccc atcctccaat ttcttctatg atacttctcc
     2761 tcaatataaa gttagttcct ggtattgtac atagattaaa aagttgccat ctaccagctt
     2821 gtgacatcca ttgaaagctt aatgtttcta tatttataaa tttggttaat aggtttttat
     2881 ttttatttcg agtcctaaat ttcccaataa cagcacctaa ttcatcattc attactatag
     2941 tttgtactag atatctaagt gcatttttat ctggtgtgtt atctgcatca taaacagcta
     3001 taaaatctcc ttttgaaatc gtatatccaa tatttaatgc attagacttt ccttttccac
     3061 ctgttaaact atctgtattt attatagtga aattataatt gttgtaccta tcctttatat
     3121 tttctaaaat ttctttacta ttatcagaag aattgtcatt tataacaata agttccatct
     3181 tactcttagg ataattgagt aatagcaaag actccacagt tctacctatc actttagctt
     3241 cattatgagc aggaacaagt attgatatca taggatattc atcaatttct tttatttctt
     3301 tatcaaagtt tttaaaataa aatatatatc cacccatagc taaaataata tttataagca
     3361 ataaagacca tatactaaac aaactaaata cgaacaa
//
LOCUS       DAELLV010000303         3389 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.303, whole genome shotgun sequence.
ACCESSION   DAELLV010000303 DAELLV010000000
VERSION     DAELLV010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.303"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(959..1369)
                     /locus_tag="KPD58_003435"
     CDS             complement(959..1369)
                     /locus_tag="KPD58_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0267161.1"
                     /translation="MKIKKSLISAMMVSAMVLGGTGAVFANGNDSSNIALPKFESLIK
                     SGITDPTELEKYGVEHYTYEEYAKHIQELKDYAKDPNAVKDVSQKDLEETIKKMEQEL
                     EKIKTEGLKIMKPITIENEDGSQISIAYNYAAID"
     gene            complement(1585..2967)
                     /locus_tag="KPD58_003436"
     CDS             complement(1585..2967)
                     /locus_tag="KPD58_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBG0267162.1"
                     /translation="MFIKLLEMSFTSGVLLLIVIFIRACLFKKLPKKVFVLLWLIVLF
                     KLLIPVSLNSGFSIYSIIESTFDVELGNTSTENYTLKKASDSTGKNNSDEFEIMSNIE
                     NIMMLEMLGILSFIVTIMFSILLCFRYFKIINLFKTSIHIKNHEFIKKWMCKNSRFRR
                     FIVCISEFTRTPLTSGILKPRIILPKQMNYANNQTLDFVLTHEMVHINSFDNLLKLFM
                     LVALVVHWFNPLVWVMFFLLNKDIEFSCDEKVMSILGEEYRADYATTLISLAEENNPF
                     SMTICSGFGKTKIEKRIVNIMKFKKSTYVTFLVSTVLVCGTGLVFATENKEINEVESF
                     GLTKNEQSDKIGFSISSKSTDLYSPKVESDILVSKAKSDGETHFSTAKVSVIEFREHE
                     ESSMSKVTNSENSEETYVNKSSNSKDVLYTDSGEVSIERSGYNDNRFVISRKGNLLAV
                     NKEQYRTWIE"
     gene            complement(2978..3337)
                     /locus_tag="KPD58_003437"
     CDS             complement(2978..3337)
                     /locus_tag="KPD58_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0267163.1"
                     /translation="MEIKLFDSELKVMEVLWERGESKAGELVKILKEETGWNRNTTYT
                     VIKKLVSKGAIERIEPNFVCKALISKEEVQKQETETLINKMFGGSSEKFLSAFINKEN
                     LSSDEITKLKQIVEKLK"
BASE COUNT         1121 a          577 c          302 g         1389 t
ORIGIN      
        1 tgatattctt atctaccatt atctatatat ttattttacg attactcttt caccctaacc
       61 ctttaactat ctattttttc ttactattct tatccacatc atccatatat ttattttaca
      121 attactcttt cactctaacc ctttaactat atattttttc tgatattctt atctaccatt
      181 atctatatat ttattttacg attactcttt caccctaacc ctttaactat atattttttt
      241 ctggtattct tatctactat tatccgtata tttattttat gattactctt ttaacctacc
      301 ccctatactt tatagtaata tgtttttaac ctttttcatt attcccaata tgctttataa
      361 ctacaaatac tctcccccct taaaaataat aataaaatta atcaataaat ttataatcat
      421 attattactc tatgtattaa gcaattacta tgccaataaa ctttgatttt tttaaacaaa
      481 tcttaaaaat cctaaaatgc taattttctt taacttttaa tgaatctaat agataacatt
      541 taattttcgt tatacttttc agaatatttc tattattatt tttaagaaaa aaaaataatg
      601 ttaacatttc ttaacataca ttaaaaaatg ttaacattat aaattgggtt taatactttt
      661 actttgactc gatttacttt tattctactt cttactttat ttttaactta gactttattt
      721 ttttattact tatttgagtc ttatttattt tattctcact ccatatgttc aattacattt
      781 gtctaaattc tttgatactt ttattacata attttaacaa gcttaagtgt tatttgggta
      841 gtgcagttga taattattta aataatcatc aactgcatgt ttatatcatt acagtagctt
      901 aatgtattta atcatactta cttaggattt tgaaatatct tagagattat atttaacatt
      961 agtcaattgc tgcataattg taagcaattg aaatttgaga cccatcttca ttttctatag
     1021 tgataggctt cataattttt aatccttcag tttttatctt ttctaattct tgttccattt
     1081 tcttaattgt ttcctcaagg tctttttgac ttacatcttt gactgcatta gggtctttag
     1141 catagtcttt taattcttga atatgtttag catactcttc ataagtataa tgctcaacac
     1201 catacttttc caattcagta gggtcagtta ttcctgattt tattagagat tcaaatttag
     1261 gtaaagcaat attagatgaa tcatttccat ttgcaaatac tgctccagtt cctcctagta
     1321 ccattgcaga taccatcatt gcacttataa gggatttttt tattttcata accaatcttc
     1381 ctttcttaga gatttatttg ggtagtgtag tttaaaatga ctattgcaat agttatcaac
     1441 cacattctta gactatcaca gtagtcatgt ttatgtcaag cctttttaaa aaacttttca
     1501 ataaaaaata ctatctcaaa acaaaattaa ttattttgag atagtatttt aattctaaat
     1561 cttttatatt actaagattt atggttactc tatccatgtt ctatattgtt ctttattaac
     1621 tgctaacaaa ttcccttttc ttgatatgac aaagcgatta tcattatagc cacttcgctc
     1681 aattgaaact tctcctgaat cagtataaag tacatcctta ctattactag atttatttac
     1741 atacgtttct tcactattct cagaattagt tactttgctc atagatgatt cttcatgctc
     1801 tctaaattca attacagata cttttgccgt actaaaatga gtctctccat cacttttagc
     1861 tttactaaca agaatatcag attctacttt gggagaatat aaatcagttg atttactact
     1921 tattgaaaat ccaattttat cactttgttc attttttgtt aaaccaaaag attcaacctc
     1981 attaatctct ttattttctg ttgcaaaaac aagacctgtc ccacaaacta atacagttga
     2041 aactaaaaaa gtaacataag ttgatttttt aaatttcata atattaacta ttctcttttc
     2101 tatttttgtc tttccaaatc cactacatat tgtcattgaa aatggattat tctcctctgc
     2161 tagacttatt agtgtggttg cataatcagc cctatattct tcaccaagta tactcataac
     2221 cttttcatca caactaaatt ctatgtcttt atttagtaaa aaaaacatca cccagactaa
     2281 gggattaaac caatggacaa caagagctac aagcataaat aactttaaca gattatcaaa
     2341 gctattaata tgaaccattt catgagttaa aacaaagtct aaagtttgat tattagcata
     2401 attcatttgc tttggcaaaa taattcgtgg ttttaaaata ccagatgtaa gtggtgtcct
     2461 agtaaattca gatatgcaaa caataaatct tctaaaacgg ctattcttac acatccattt
     2521 tttaatgaac tcatgatttt ttatatgaat tgaagtttta aaaagattaa ttattttaaa
     2581 atatctaaag cataaaagta tgctaaacat aatagtaact ataaaagaca aaatgcccaa
     2641 catttccaac atcataatat tttcaatatt agacattatt tcaaattcgt cagaattgtt
     2701 tttacctgta gagtcactag ctttcttaag agtataattt tctgttgatg tattacccaa
     2761 ttccacatca aaagtcgatt caattatact ataaatacta aaaccagaat ttaaacttac
     2821 tggtattaga agtttaaata aaacaatcaa ccacaataat acaaatacct ttttaggtaa
     2881 ttttttaaat aaacatgctc ttataaaaat aactattaaa agtaatactc cagaagtaaa
     2941 actcatttct aacaatttaa tgaacataaa tcttacctca ctttaatttt tcaacaattt
     3001 gtttaagttt tgtaatctca tcacttgaaa gattttcttt attaataaaa gctgaaagaa
     3061 atttttctga agaccctcca aacattttat taattaaagt ttctgtttcc tgtttttgta
     3121 cttcctcttt gcttattaaa gctttacaaa caaaattcgg ctcaattctc tctattgcac
     3181 cttttgacac taattttttt ataacggtat acgtagtgtt tctattccat cctgtttctt
     3241 cttttaaaat ttttactaac tctccagcct tgctttctcc tctctcccat agaacttcca
     3301 ttactttaag ttctgaatca aataatttta tctccatata aaatcgctcc tcaaatatag
     3361 ttatcacaat agtcattact ataataaaa
//
LOCUS       DAELLV010000304         3341 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.304, whole genome shotgun sequence.
ACCESSION   DAELLV010000304 DAELLV010000000
VERSION     DAELLV010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.304"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..968
                     /locus_tag="KPD58_003438"
     CDS             <1..968
                     /locus_tag="KPD58_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBG0267164.1"
                     /translation="NNQYKGKIKSANLSVDYEINQVMKDIDKLGLNTVNVPIIINVES
                     INSDIMSIDNNSKEKAIKLIKKLNKKGISTILEAYPWIENGKLYETDWNPINKKRFFY
                     TWQNAILNELIIDVANPLDVNVLNIGSNFVHLEEYQQNWGEIIDFVQSKFDGLVTYRT
                     NWWYTKKDDLSSKLFYEQKLNNNFFDKLDFISIAAYFELSNKPVNTVDELISALHSST
                     VNNRGQNIKQEIYNLYKAHRKPIFFGELGFSNRESASSQPWNHTPSKAVNGEEQARCF
                     EAYKKVFENEDWINGFSVFCVGKIDDEKNFYPSKESIKVIKSWYE"
     gene            1022..2146
                     /gene="wecB"
                     /locus_tag="KPD58_003439"
     CDS             1022..2146
                     /gene="wecB"
                     /locus_tag="KPD58_003439"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HBG0267165.1"
                     /translation="MNDIKVMTVFGTRPEAIKVAPLIKELEKRENIKSIVCVTAQHRE
                     MLDQVIETFNINVDYDLDIMEKGQSLNDITCKILNKLPLILNKENPNIILVHGDTTTT
                     LATSLTAFYNKTLVGHIEAGLRTYDKYSPFPEELNRQLTGIIADMHFAPTNLARKNLI
                     SEGKPNNNIFVTGNTAIDALKMTIKENYNHPIIDEIGNDRMILLTSHRRENLGKPMKN
                     IFRAIKRIVDDFEDVQIVYPIHLNPKIRTIADEIFGKFPEKIHIIEPLDVADFHNFLN
                     KSYMIMTDSGGIQEEAPSLGKPVLVLRDKTERTEGIEAKTLKLVGTNEDRIYNSVSDL
                     LINKDNYVQMSKASNPYGDGNASKYIVDIIIKKFNCKYLN"
     gene            2224..3198
                     /locus_tag="KPD58_003440"
     CDS             2224..3198
                     /locus_tag="KPD58_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBG0267166.1"
                     /translation="MARKLIKNLGKSKSVKRVKLLFKKIFITVFIVASIVAIFNITKY
                     FEELYKVRDLKSTKIEYYMDVADEAGDGKVQLSWKALLAIDMVIHDEDLSNIKKKDTL
                     DIGEKFIVEDKNGKGEKVYKVKKFNKVLSELKFDSSQKSRARKYMKDLEYTYLGNKQL
                     DSSDEKIKFIKKLEDSAIREYIDYGILPSITIGQAILESGWGNSKLTKQSNNLFGIKA
                     DKAWKGKSVEISTSEHYNEKIVASFRSYNSLQDSVKDHSLFLINNKRYRKHGLFEAKD
                     YISQAQALENAGYSTAEDKKGNRIYAELLIDVIRSYNLQLIDNKVETK"
BASE COUNT         1398 a          330 c          588 g         1025 t
ORIGIN      
        1 taaataatca atataaaggt aagattaaat cggccaattt atctgtagat tatgaaataa
       61 atcaagttat gaaggatata gataaattgg gattgaatac agtaaatgta cctataatta
      121 taaatgtaga gtccattaat tctgatatta tgtcaataga taataatagt aaagaaaagg
      181 caattaagtt aataaaaaaa ttaaataaaa aaggtatttc aacaatatta gaggcatatc
      241 catggattga aaatggaaaa ttgtatgaga ctgactggaa ccctataaat aaaaaaagat
      301 ttttttatac ttggcaaaat gccatattaa atgaacttat aattgatgta gcaaatccac
      361 tggatgtaaa tgttttaaat attggttcta attttgtaca tttggaagaa tatcaacaaa
      421 attggggtga aataatagat tttgtacaat ccaaatttga cggtcttgta acgtacagaa
      481 ctaattggtg gtatacaaaa aaagatgatt taagtagtaa gttgttttat gaacaaaagc
      541 tgaacaataa tttttttgat aagttagatt ttatatctat agcagcatat tttgagttaa
      601 gtaataaacc tgttaataca gtggatgaat taatttctgc cttacactca tctactgtaa
      661 acaatagagg acaaaatata aaacaagaaa tatataacct ttataaagct catagaaaac
      721 ctatattttt tggagaatta ggtttttcaa atagagaaag tgcaagttct cagccgtgga
      781 atcatactcc ttcaaaagct gtaaatggcg aagaacaagc aaggtgtttt gaggcatata
      841 agaaagtttt tgaaaatgaa gactggataa atggtttttc tgtgttttgt gtaggtaaaa
      901 tagatgacga aaaaaatttt tacccatcaa aagagagtat aaaagttatt aagtcttggt
      961 atgaataatt ttgaaagtgt agaatttata tagattaaat agatgattag gagagatata
     1021 aatgaatgac atcaaggtta tgactgtatt tggaactaga cctgaagcta tcaaagtagc
     1081 acctttaata aaggaacttg aaaaaagaga gaatataaaa agtattgttt gcgttacagc
     1141 tcaacataga gaaatgcttg accaagttat agaaaccttt aatataaatg tagactatga
     1201 tttggatata atggaaaaag gacaaagcct gaatgatata acatgtaaga ttttaaataa
     1261 acttcccttg attttaaaca aggaaaatcc aaatataata ttggtacatg gagatacaac
     1321 cacaactcta gctacaagtt tgactgcatt ttataataaa actcttgttg gtcatataga
     1381 agctgggctt agaacctatg ataaatattc tccttttcca gaggagctaa atagacaact
     1441 tacaggtatt attgctgata tgcactttgc tcctactaat ttggctagaa aaaacttgat
     1501 ttctgaagga aaacctaata ataatatatt tgtaactggt aatactgcta ttgatgcttt
     1561 aaagatgaca ataaaagaaa actataatca tcctataatt gatgagattg gtaacgatag
     1621 gatgattttg ttaacctctc acagaagaga aaatttgggt aaacctatga aaaatatatt
     1681 cagagctata aagcgaatag tagatgactt tgaagatgta cagatagtat atcccataca
     1741 tttaaatcct aaaatacgaa ctatagctga tgagatattt ggaaaatttc ctgaaaaaat
     1801 tcatattata gagcctttag atgtagctga ttttcataac tttttaaata aatcttatat
     1861 gataatgact gatagtggag gaatacagga agaagctcca tcattaggta agccagttct
     1921 tgtgttgcgt gataaaacag aaagaacaga aggaattgaa gcaaagacac ttaaattagt
     1981 aggtactaat gaagatagaa tatataattc agtgagcgat ttattaataa ataaagataa
     2041 ctatgttcag atgagtaaag catctaatcc atatggagat ggaaatgcaa gcaaatatat
     2101 agtggatata ataattaaaa aatttaattg caaatattta aattagataa ggcctaaaac
     2161 attaactaat tgatataaga tttatgataa tattaagtaa tacaattttt aagggggagt
     2221 aatatggcta gaaaattaat aaaaaatttg ggtaaaagta aaagtgtaaa gagagtgaaa
     2281 cttttattta agaagatttt tattactgta ttcatagtag caagtatagt tgctattttt
     2341 aatataacga aatattttga ggaattatac aaagtaaggg atttaaaaag tactaaaatt
     2401 gaatactata tggatgtggc tgatgaagca ggagatggaa aggtccaatt aagttggaag
     2461 gccttacttg ctatagacat ggtaatacat gatgaagatt taagtaatat aaaaaagaaa
     2521 gacacattgg atatagggga aaagtttata gtagaagata aaaatggtaa aggcgaaaag
     2581 gtgtataagg taaaaaagtt taataaggta ttaagcgaat tgaaatttga ctcttctcaa
     2641 aaaagtagag caagaaaata catgaaagat ttagaataca cataccttgg aaataaacaa
     2701 ttagatagta gtgatgaaaa aattaaattt ataaagaagt tagaagactc agctataaga
     2761 gaatatattg attatggaat attgccctct ataacaattg gacaagctat attagaatct
     2821 ggttggggaa attctaaact tacaaaacag agtaataatt tatttggtat aaaagcagat
     2881 aaagcatgga aaggaaaaag tgtagaaatt tcaacttcag agcattataa tgaaaaaatt
     2941 gtagctagtt ttaggtcgta taattcatta caagattctg tcaaagacca cagtttattt
     3001 ttaattaata ataaaagata tagaaaacat gggttgtttg aggcaaaaga ttatattagt
     3061 caagctcaag cattagaaaa tgcaggatat agtacagcag aagataaaaa aggaaatcgt
     3121 atatatgcag agctattaat agatgttatt aggagttata atttgcaatt gatagataat
     3181 aaagttgaga caaaataatt gttaacttta tatgaggaga taacaagata agattttttt
     3241 ctacaaaaag aatagaaaaa ttgtaaaaaa aactaaaaaa atgtattgac ttgttattta
     3301 aaaggtgata ttattattac tgtactcgaa agagaacaaa a
//
LOCUS       DAELLV010000305         3315 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.305, whole genome shotgun sequence.
ACCESSION   DAELLV010000305 DAELLV010000000
VERSION     DAELLV010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.305"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            230..1462
                     /locus_tag="KPD58_003441"
     CDS             230..1462
                     /locus_tag="KPD58_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBG0267167.1"
                     /translation="MQEREELVLNPVPKEERVGWLAPLFNMLGSNIAISELMVGGTLI
                     LGMTLSNMIITSIIGNLILVAIIMIQGYIGYKEGLNTYVLAKGAFGEIGGKYLISLLL
                     GITSFGWFGVQAGVAGLSVQKIFPSVNLTLVTVILGLFMVVFALYGFKAMAKFNYLVI
                     PPLMILMVWGVFKAFSTYGVEAIYNYTPQTTMSMVEGLNIVVGLVIVGAIISPDQLRY
                     TRRVKDIWIISFLGLGIISLFQQVAAGVMSMGAPTWDITEVLANLGFGWVAFVILILA
                     SWSTNVSNAYSGGLALKTIFPNVKRNVLTLVAGLIGTIIAATGIIFKFQSFLSFLGIA
                     VPAIAGIMWCEYYFIQGRTYKHREGINWIAVISWLIGFAASYYSSKINFLIPPINGIV
                     VSMVIYYILMNCFGIKDK"
     gene            1476..2282
                     /locus_tag="KPD58_003442"
     CDS             1476..2282
                     /locus_tag="KPD58_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HBG0267168.1"
                     /translation="MGRKVKIGIIQQHSVLGNVKKNIEKAVEMIDDLGKQGADIICLP
                     ELFATGYNLESLGGVKTLELIREHNKYIEESMSEAAKRNNVYLISPYGTLEKGSTHVY
                     NSAVIFDRKGKIMGEYCKNHLWSLEAVYFKGGEKVEVYDADFGRFGVMICYDAGFPEV
                     SRELTLKGSEIIFIPSAWRIQDEDMWDLNVSQRALENTVYTVGVNLVSNDSNLILFGK
                     SKICNPRGTVITQLDTYREKYVLSEIDLDEIKKYREDIPYLKDRKSSYNI"
     gene            complement(2394..3263)
                     /locus_tag="KPD58_003443"
     CDS             complement(2394..3263)
                     /locus_tag="KPD58_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG0267169.1"
                     /translation="MDLLHLKYFQTVARMEHITKASHKLNIAQPALSKTISSLEKELG
                     VQLFDRKGRYIVLNEYGRLFLKRVDSILDLVESSKKELQDTSLENSGEVKILSPAAAN
                     VLPSLLSNFRKLYPNITFNVSHTLPSSYKKSDFDLYISSSFTKLNSENSITLTCEEIL
                     LGVSINHPLSLKDEVYLSEVSDENFIVITKGENYREVIDILCESANFKPKIAFESDSP
                     YTIYALIKSLQGVGFICGKSWGLSQDPEIKLLHIKDIEFKRYLNLSWFSENYESKAVL
                     LFKNFLINYFKNI"
BASE COUNT         1191 a          386 c          582 g         1156 t
ORIGIN      
        1 aacaaatttg aaataagtct aatatatgac ttaatagatt aaattaaaat aataaatttg
       61 gactatttat ctatttataa cttaaattat atcaaattga taacaaacta gatattatac
      121 ttttttgtag aatagaatta caattatata aagaaaaatt ctattaatag tgaaaaaaaa
      181 gtagaaaaaa gtagaaatta ttcttttata aagaaaggga gggataatat tgcaagagag
      241 agaagaatta gttttaaatc cagttccaaa agaggaaaga gttggatggt tagctccatt
      301 gtttaatatg cttggttcta acatagctat atctgaacta atggttgggg gaactttaat
      361 tttaggaatg actctttcta atatgattat tacatcaatt atagggaact taatattagt
      421 tgcaattatt atgatacaag gttatatagg ttacaaagaa gggttaaata cgtatgtatt
      481 agctaagggg gcatttggag agataggagg aaagtatctt atatctctat tacttggtat
      541 aactagtttt ggatggtttg gtgtacaagc aggggttgca ggactatcag tacaaaagat
      601 attcccaagt gtaaacctaa cattagttac agttatactt ggattattta tggttgtttt
      661 tgcactgtat ggctttaaag ctatggctaa atttaattac ttagttatac caccacttat
      721 gatattaatg gtgtggggag tttttaaagc tttttctact tatggagttg aagctattta
      781 taattatact cctcaaacaa ctatgagtat ggttgaaggt cttaatattg tagtaggtct
      841 tgttatagtt ggtgcaatta tatcacctga ccagttaaga tatacgagac gagtaaaaga
      901 catttggatt atcagttttt taggtctagg gattatttca ttattccaac aagttgcagc
      961 tggtgtaatg tctatgggag cacctacttg ggatataaca gaagtattgg ctaatttagg
     1021 ttttggatgg gtagcgtttg ttattttaat acttgcatct tggtctacaa acgtatcaaa
     1081 tgcttattct ggtggattag cattaaaaac tatttttcca aatgtaaaaa gaaatgtttt
     1141 gactttagtt gcaggtctaa taggtacaat aatagcagct actggtataa tatttaaatt
     1201 tcagtcattt ttatctttct tgggaatcgc agttcctgca atagcaggaa ttatgtggtg
     1261 tgagtattat tttatacaag gaagaactta taagcataga gaaggaataa attggattgc
     1321 agtaataagt tggttaatag gttttgcagc gtcgtattat agttcaaaaa taaatttctt
     1381 aatacctccg ataaatggaa ttgtagtgag tatggttatt tattatatat taatgaattg
     1441 ttttggaatc aaagataaat aaagggggca attacatggg tcgtaaggtg aaaataggta
     1501 taattcaaca acacagtgta cttggaaatg ttaaaaaaaa tatagaaaaa gcagtagaga
     1561 tgatagatga cttaggaaaa caaggggcag atataatttg tttaccagaa ctatttgcta
     1621 caggatataa tttagagtct ttaggtggag ttaaaacatt agaattaata agagaacata
     1681 ataagtacat agaggaatca atgtcagaag ctgctaaaag aaataatgta tacctgatat
     1741 ctccatatgg aaccttagaa aaagggtcta cacatgttta taattcagca gttatatttg
     1801 atagaaaagg taagattatg ggagaatact gtaaaaatca tctgtggtca ttagaagcag
     1861 tatactttaa aggaggggaa aaagtagagg tatatgatgc tgattttggt agatttggtg
     1921 taatgatatg ttatgatgca ggttttccag aagtatccag agaattgaca ttaaaaggtt
     1981 ctgaaataat atttatacca tctgcatgga gaatacaaga tgaagatatg tgggatttaa
     2041 atgtatctca aagagcttta gagaacactg tatatacagt aggtgtaaat ttagtaagta
     2101 atgactctaa tttaatacta tttggaaaga gtaaaatatg caatcctaga ggtactgtaa
     2161 taactcaatt agatacatac agggaaaaat atgtattatc agagattgat ttagatgaaa
     2221 taaaaaagta tagggaagat atcccctatt tgaaagacag aaaaagtagc tataacatat
     2281 aattctatac tataatacaa gttcaatata aaatgataaa aagaggatgt atcaatatta
     2341 agattaaatt tattatactt tgatatatcc tctttaatat tatcaacttt gatttaaata
     2401 tttttaaaat agttgataag aaagtttttg aacaacaaca cagctttaga ttcatagttt
     2461 tcgctaaacc atgaaagatt taaatatctt ttaaattcaa tatctttgat atgtaacaac
     2521 ttaatctcag ggtcttgaga taatccccat gattttccac atataaatcc aactccttga
     2581 agtgatttta taagagcata aattgtatat ggtgaatcag actcaaatgc tattttgggt
     2641 ttgaaattag ctgattcgca caggatatca attacctctc tataattctc acccttcgtt
     2701 ataactataa agttttcatc tgaaacttcg cttagatata cttcatcctt taatgataaa
     2761 ggatggttta ttgaaactcc taatagtatt tcttcgcatg ttaaagttat gctattttct
     2821 gagtttagct ttgtaaatga agaagatatg tacaaatcaa aatcactttt tttataagat
     2881 gatggcaaag tatgtgacac attaaaagta atattgggat atagttttct gaaattactc
     2941 aaaagtgatg gtaacacatt agccgctgca ggagataata tctttacttc tcctgaattt
     3001 tctaaagaag tgtcttgtaa ctcctttttt gaactttcta ctaaatctaa aatgctatct
     3061 actcttttta gaaacaatct tccatactca tttaaaacaa tatatcttcc ttttctgtca
     3121 aataactgaa ctccaagttc tttttctaag gaagatatag ttttgcttaa agctggctgt
     3181 gctatattta acttgtgtga tgcttttgtt atgtgctcca ttctagcaac agtttgaaaa
     3241 tacttaagat gcaataaatc catagtataa aatacacctc ttttaatatg acttgagtta
     3301 tatcaatttt ataat
//
LOCUS       DAELLV010000306         3275 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.306, whole genome shotgun sequence.
ACCESSION   DAELLV010000306 DAELLV010000000
VERSION     DAELLV010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.306"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            124..633
                     /locus_tag="KPD58_003444"
     CDS             124..633
                     /locus_tag="KPD58_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HBG0267170.1"
                     /translation="MNSNERRSKLIDILKESKHPVKGGTLAELLNVSRQVIVQDIALI
                     RARGFEIIATPQGYIIYNQPYFTKQIKCKNHKDSKEIYDELKIIVDLGGIIKDVIVNH
                     PTYGQITAELNIYSKMDIDNFMKKVETNEFKQLSVLTECSHIHTIEAIKEETIEAIVK
                     ELKDNGILS"
     gene            645..1526
                     /locus_tag="KPD58_003445"
     CDS             645..1526
                     /locus_tag="KPD58_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HBG0267171.1"
                     /translation="MNFLSLAKTASTNSNITKRTLENMLDNFSDNIPNIIYAIIVFVI
                     GIYISKIVRRMVSKFLIKYGMSKGVNNFIVYGIYISMLSIISLISLGIIGIQTTSVVA
                     VLGAAGFSIGLAFKEILSNLGSGMIILFFKPFNIGDYIQGSGVEGTVSDIQIFSTVLK
                     TPDNKTIIIPNFQLTSNNIINYTHQNKRRIDFSYNISYDSDIDVVKSILNEIFTNEKR
                     ILNDPKPIIGLNSIGNNTMQIVARPWVKTDDYWDVYFDVMEKVKNKFDENDIKVPFVP
                     SSLLFNNTGNLNTMDKR"
     misc_binding    1654..1859
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1985..3256
                     /gene="serS"
                     /locus_tag="KPD58_003446"
     CDS             1985..3256
                     /gene="serS"
                     /locus_tag="KPD58_003446"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBG0267172.1"
                     /translation="MLDIKRIRENLDDIKKAMERRGEREFDLDAVVELDNKRREILQE
                     VEVMKNELNVNSKKIPQLIKEGKDVTEEKARLKELSDKIKGIDEKVKEVEAKMEYTLM
                     RIPNVPHPEVPQGETDEDNVQIRTWGEPTKFDFEHKAHWDIGTGLGILDFERAGKITG
                     SRFTLYRGLGARLERSLMNFFLNTHTAKHGYTEVLPPFMANRNSFIGTGQLPKFEEDM
                     FKIEGLEYFLIPTAEVPVTNIHANEILDVAELPIKYCAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVELVKFVKPEDSYNELESLTHDAETMLQMLGLPYRVVKICTGDLGFTAAFK
                     YDLEVWMPSYNRYVEISSCSNFEDFQARRAGIRFKRDKKSKAEYVHTLNGSGLAIGRC
                     LAAILENYQQADGSVVVPEELRPYMGVDVIK"
BASE COUNT         1334 a          314 c          576 g         1051 t
ORIGIN      
        1 tcttttttat ttattataaa agttaagatt aaatagaata taaagtcaat attgatttat
       61 aataagataa aatataaaat tatatagtat aggtaaagtt tttatttgga aagaaggtgt
      121 aatatgaatt ctaatgaaag aagaagtaaa ttaatagata ttttaaaaga atctaagcat
      181 cctgtaaaag gtggaacact agctgaacta ttaaatgtaa gtaggcaagt aatagttcaa
      241 gatatagccc ttataagagc tagaggattt gaaataatag ctacacctca aggatatata
      301 atatataatc aaccgtattt tacaaaacaa ataaagtgta aaaatcataa ggattctaaa
      361 gaaatttatg atgaattaaa gataattgtg gatttaggtg gtattataaa agatgtcatc
      421 gtaaatcatc caacatatgg tcagataact gcagaactta atatttattc taaaatggat
      481 atagacaact ttatgaagaa agttgagaca aatgaattta aacaattatc ggttctgaca
      541 gaatgtagcc atattcatac aattgaggca ataaaagaag aaactataga agctatagta
      601 aaagagttaa aggacaacgg aatattatca taaggaggag ttgaatgaat tttttatcat
      661 tggctaagac tgcctcaaca aatagcaata taactaagag aactttagaa aatatgctcg
      721 ataattttag tgataatata ccaaatatta tatatgcaat aattgtattt gtgataggta
      781 tttatatatc taaaatagta agaaggatgg tttcaaagtt tctaatcaaa tatggaatga
      841 gtaaaggtgt aaataatttt attgtatatg gaatatatat atctatgtta tctattatat
      901 cattgataag cttaggtatt atagggatac aaactacatc agttgtagct gtgttaggag
      961 ctgctggatt tagtataggt ttagctttta aagaaatatt atcaaattta ggctctggta
     1021 tgattatttt attttttaag ccatttaata taggtgatta tattcaaggt tcaggtgtag
     1081 aaggtacagt ttctgacata caaatattta gcactgttct taagacgcca gataataaaa
     1141 ctataattat acctaatttt caactgacaa gtaataatat aattaattac acacatcaaa
     1201 acaaaagaag aatagatttt tcatataata tttcatatga tagtgatata gatgtagtta
     1261 agtctatatt aaatgaaatt tttacaaacg aaaaaagaat attaaatgat ccaaagccta
     1321 taataggttt aaactctata ggaaataaca ccatgcaaat agttgctaga ccttgggtaa
     1381 aaactgatga ttactgggat gtatattttg atgtaatgga aaaggtaaaa aataaatttg
     1441 atgagaatga tataaaagtt ccatttgttc cttcttcatt attatttaat aatacaggta
     1501 atctaaatac aatggataaa agataaggtt ttaattaaat agaaaaaatg aatttaatat
     1561 aaaaaattaa agaaaattct aaaaaaattt gaataatagt attgaaagat gttaatatat
     1621 ataatataat gattatcaaa taaaacaatt agttaaaaag ctgtgaagga aaatagtaaa
     1681 aaacagattg tattcagaga atgagtggat ggtgaaaact cattacaatg gtttttgaat
     1741 ctactctaga gcttctagtt aaagctagac gtttacctac gttataggtc tgagaggatt
     1801 atacatttta gtatagtcaa ttagggtggc aacgcggata aaagattttc gtccctttta
     1861 tagggtaaac ggaggtcttt ttttattttt aaaatttgtt taaactaaga gtttacaagt
     1921 ataatgtttg taaattaagt tggaattata acttatatta tcaaaattat taggaggaat
     1981 taatatgtta gatattaaaa gaataagaga aaatctagat gatatcaaaa aagctatgga
     2041 aagaagaggt gaaagagaat ttgaccttga tgctgtagtg gaattagata ataaaagaag
     2101 agaaatactt caagaagttg aagttatgaa aaatgagtta aatgtaaatt ctaaaaaaat
     2161 acctcaatta ataaaagaag gaaaagatgt aacagaagaa aaggcaagat tgaaagagtt
     2221 atcagataag ataaaaggta ttgatgagaa agtaaaagaa gtagaagcaa aaatggaata
     2281 tacattaatg agaataccaa atgttcctca tccagaggta ccacaaggtg aaacagatga
     2341 agacaatgtt caaataagaa cttggggaga acctactaaa tttgattttg agcataaagc
     2401 gcattgggat ataggtacag gtctaggtat tttagatttt gaaagagcag ggaaaataac
     2461 tgggtcaagg tttactttat ataggggatt aggagctaga ttagaaagat ctttaatgaa
     2521 tttcttttta aatactcata cagctaagca tggctatacg gaagtcttac ctccatttat
     2581 ggccaataga aatagtttta tagggactgg tcaacttcct aaatttgaag aagatatgtt
     2641 taaaatagaa ggattagagt atttcttaat accaacagct gaagttcctg ttactaatat
     2701 acatgctaat gaaatattag atgttgcaga gcttccaatt aaatactgtg catatactcc
     2761 ttgttttaga tctgaagcag gttcagcagg aagagataca agaggtttag ttagacaaca
     2821 tcaatttaat aaagttgaat tagttaaatt tgttaaacca gaagattctt ataatgaatt
     2881 agaatcatta actcatgatg ctgaaactat gttacaaatg ttaggtcttc catatagagt
     2941 tgtaaaaata tgtacaggag atttgggatt tacagctgca tttaaatatg atttagaagt
     3001 ttggatgcca agttacaata gatatgtaga aatatcttct tgttcaaatt ttgaagattt
     3061 ccaagcaaga agagcaggaa taagatttaa aagagataaa aaatcaaaag cagaatatgt
     3121 tcatacatta aatggttctg gattagcaat aggaagatgt ttggctgcta tattagaaaa
     3181 ttatcagcag gcagatggtt ctgtagtagt tccagaagaa ttaagacctt atatgggtgt
     3241 tgatgttata aaataaattt atattaattg tatat
//
LOCUS       DAELLV010000307         3263 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.307, whole genome shotgun sequence.
ACCESSION   DAELLV010000307 DAELLV010000000
VERSION     DAELLV010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.307"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(113..685)
                     /locus_tag="KPD58_003447"
     CDS             complement(113..685)
                     /locus_tag="KPD58_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBG0267173.1"
                     /translation="MWIYQKTIQHPVNIKTCDPRMAKFLITQFGGPNGELAASLRYLS
                     QRYTMPTGNMRALLTDIGTEELAHVELICTMVYQLTSDASPEELKAAGLGSNYAQNGY
                     GIYPTDSNGVPFDVRPIAVMSNPVTDLHEDMAAEQKALATYYQLINLTDDVDVIDVLK
                     FLGQREIIHYQRFGEALMDAYELEESQKMF"
     gene            complement(714..980)
                     /locus_tag="KPD58_003448"
     CDS             complement(714..980)
                     /locus_tag="KPD58_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HBG0267174.1"
                     /translation="MKDSSRAELMRKVQETSFACVDMNLYLDNHPDDKNAINTYNSLC
                     NQFAQARYAYENKYGPLTNFGYAPSRCPWQWADQPWPWDREFNY"
     gene            complement(980..1189)
                     /locus_tag="KPD58_003449"
     CDS             complement(980..1189)
                     /locus_tag="KPD58_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HBG0267175.1"
                     /translation="MENKTRDIKKNCGPSLARPYINNQIFTEMYCLSDALKFGTIFPE
                     LNLLESEMYNKELYAKPKKYRGGRR"
     gene            1458..2318
                     /locus_tag="KPD58_003450"
     CDS             1458..2318
                     /locus_tag="KPD58_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBG0267176.1"
                     /translation="MSELSGKGCEIIVPFEERLPVRDIEKSIIKKYRKNLWSKFMKAI
                     RDYKLVEEGDKIAVAISGGKDSILMAKMFQELKKHGQVNFDVEFIAMDPGYHANIRQL
                     LIDNCEYLNIPIHLFDSRIFEIADEIAKDYPCYMCARMRRGALYSKAEELGCNKLALG
                     HHYDDVIETTMLNLLCAGNFKTMLPKLNSTNFEGIKIIRPLYYIREEHIIRFIQNSGI
                     WPLNCACMVAAKKTGNKRYEIKDLIKSLESNFKNVEKSIFKAAENVNLDSVLGWQKDG
                     EKHSFLENFE"
     gene            2357..>3263
                     /locus_tag="KPD58_003451"
     CDS             2357..>3263
                     /locus_tag="KPD58_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBG0267177.1"
                     /translation="MFILKRVYIDFEMNMPNSKSKRAVFNSDIIAIGAVMYDEKTKNI
                     DKFKSLIRPVSNEELYPHIQELTHISSEELKSAPSYEEVMRKFKKWLGIFSDIKGIYT
                     FGNLDLTCFNNTDMRSAKKNNHPRFVNNIRDLFVDIKEKYINCGMRCMNYISLKNLLE
                     FVNLEFSGDAHDPLNDVYNLFILDEAITNNVDIQNLLIIRDIIRPPFNDINSNLDNCF
                     NKFKESLYKKEGNYNIVDFSVEIIKTVRMYLLTIIDVNIQNLEIMKDISKKMDTIDKL
                     KDIEEGYFYLLEDVYFDMKDVLEDLM"
BASE COUNT         1243 a          376 c          528 g         1116 t
ORIGIN      
        1 ttatttattt tctctgttta tagaaaataa attatattta tattttttat aaattttaac
       61 acaacagtaa attataaatt atcaaataat aatagttata caaataaaaa ttttagaaca
      121 ttttttgaga ttcttctaac tcgtaagcat ccattaaggc ttcaccaaat ctttgataat
      181 gaattatttc tctttgaccc aagaatttta atacatctat aacatcaacg tcatctgtta
      241 ggtttataag ttgataataa gttgcaagtg ctttttgttc agctgccata tcctcatgta
      301 aatcggttac gggatttgac ataactgcta taggtcttac atcaaatgga acaccatttg
      361 aatctgttgg ataaattcca tatccatttt gagcatagtt tgaaccaaga cctgcagctt
      421 ttaactcttc tgggcttgca tcagaagtta actgataaac catagtacat ataagctcaa
      481 cgtgagctag ttcttctgta ccaatatctg ttaaaagtgc acgcatatta ccagtaggca
      541 ttgtatatct ttggcttaaa tatcttaaag atgcagcaag ttctccattt ggcccaccaa
      601 attgagttat aagaaattta gccattctag ggtcacaagt ttttatatta actgggtgtt
      661 gtatagtttt ctgatatatc cacatgaatt ttcaatcctc caatacttga tgtctaataa
      721 ttaaattctc tgtcccaagg ccaaggttgg tcagcccatt gccaagggca tctacttgga
      781 gcatacccaa agttagttaa aggaccgtat ttattttcgt aagcatatct agcttgcgca
      841 aattgattac ataatgaatt ataagtgttg atagcatttt tatcatcagg gtgattgtct
      901 aaatataagt tcatatcaac acatgcaaaa gatgtttcct gaacttttct cataagttca
      961 gctctagatg agtctttcat tatcttctac ctcctctata tttttttggc ttagcatata
     1021 attctttgtt gtacatttca gactctagta agtttagttc tggaaatata gttccaaatt
     1081 ttaatgcatc agaaaggcag tacatttctg tgaaaatttg attattaatg tatggtctag
     1141 ccaaagatgg accacaattt tttttgatat ctctagtctt attttccaag atgaaaacct
     1201 ccttgttgta aatagatagg taactctatc tagtgtataa tattcaatca tttaaaaatc
     1261 gttactaata ataaaatatt aaatgagaaa aataaaaaag ataataaaag aaagaggtta
     1321 tttatgttat tataagttta tgtgttattg taaactataa attatttagt aaaattaaag
     1381 taaaaattat agtattttgt ggatgaaggt agactttttt gtaaaatatt attaacataa
     1441 atatacgagg tgattagatg agtgaactat caggaaaagg ctgtgaaatt atagttcctt
     1501 ttgaggaaag acttccagta agagatatag aaaaaagtat aataaaaaaa tataggaaaa
     1561 atttatggtc taagtttatg aaggcaataa gagattataa attggttgaa gaaggtgata
     1621 agatagctgt agcgatttct ggtggaaaag acagtattct tatggctaaa atgtttcaag
     1681 agttaaaaaa acatggacaa gttaattttg atgttgaatt tattgctatg gaccctggat
     1741 atcatgcaaa tataagacaa ttacttatag ataattgtga gtatctaaat ataccaatac
     1801 atttatttga ttcaaggata tttgagatag cagatgaaat agcaaaagat tatccatgct
     1861 atatgtgtgc aagaatgagg agaggagcat tatattcaaa ggcagaagaa ttagggtgta
     1921 acaaacttgc attaggtcat cattatgacg atgtaataga gacaacaatg cttaatttat
     1981 tgtgtgctgg aaattttaaa acaatgcttc ctaaacttaa ttctactaat tttgaaggta
     2041 taaaaattat aagaccactt tattatataa gagaagaaca tattataaga tttattcaaa
     2101 atagtggtat atggccatta aattgtgcat gtatggttgc tgctaaaaaa acaggtaata
     2161 aaagatatga gataaaagac ttaataaaga gcttagaatc aaattttaaa aatgtagaaa
     2221 aatctatatt taaggcggca gaaaatgtaa atcttgattc agtacttggt tggcaaaaag
     2281 atggggagaa acattctttc ttagaaaatt ttgaataaaa atagttaaaa agaatcacta
     2341 taaacaattg gaaagggtgt ttatattgaa aagagtatat atagattttg aaatgaatat
     2401 gccaaattct aaatcaaaaa gagcagtatt taattctgat ataattgcaa tcggagcagt
     2461 tatgtatgat gaaaaaacaa aaaatattga taaatttaaa tctttaataa gaccagtaag
     2521 taatgaggag ttgtatcctc atatacaaga attaacacat atatcttctg aagaattgaa
     2581 gagtgcacca agttatgaag aggtcatgag aaagtttaaa aaatggcttg gcatattttc
     2641 tgatattaaa ggaatatata cttttggtaa tttggatttg acatgtttca ataacacaga
     2701 tatgagaagt gctaaaaaaa ataaccatcc aaggtttgta aataacatta gagatttatt
     2761 tgtagatata aaagaaaaat atataaattg tggtatgaga tgtatgaact atatatcatt
     2821 gaaaaattta cttgaatttg ttaacttaga gtttagtgga gatgcccatg acccattaaa
     2881 tgatgtatat aatttattta ttttggatga ggcaatcaca aataatgtag atatacaaaa
     2941 tcttttaata atacgtgaca taataagacc accatttaac gatataaatt ctaatttaga
     3001 taattgtttt aacaaattta aggagtcatt atataaaaaa gaaggaaatt ataatattgt
     3061 agatttttct gttgaaataa taaaaactgt gagaatgtac ttattaacaa tcatagatgt
     3121 aaatatacaa aatttagaaa ttatgaaaga tataagtaaa aaaatggata ctatagataa
     3181 attgaaagat atagaggaag gctattttta tttattagaa gatgtgtact tcgatatgaa
     3241 agatgtacta gaagatttaa tgc
//
LOCUS       DAELLV010000308         3258 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.308, whole genome shotgun sequence.
ACCESSION   DAELLV010000308 DAELLV010000000
VERSION     DAELLV010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.308"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            134..1051
                     /gene="pfkB"
                     /locus_tag="KPD58_003452"
     CDS             134..1051
                     /gene="pfkB"
                     /locus_tag="KPD58_003452"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBG0267178.1"
                     /translation="MIYTVTLNPSIDYIVKLDELKTGSTNRVKEEYVYPGGKGINVSR
                     ILKELGNDNTSLGFISGFTGEYIIRTLEEKELKTDFIKIKSGFSRINVKIKALKETEI
                     NGQGPNIDDEDIDTLYKKLDKLNQDDILILAGSIPSTLDEKLYENIMARLEKKNIKVV
                     VDATKNLLLNVLKYKPFLIKPNNDELEELFGVKLNSIEDMVKYARRLKEMGAINVLVS
                     MGKDGALLITEEDEVLISDVPKGKVKNSVGAGDSMVAGFISGYLNTGKYDYALKLGAA
                     SGSATAFSYDLAKREYIDKLVNEISVKQF"
     gene            1088..3007
                     /locus_tag="KPD58_003453"
     CDS             1088..3007
                     /locus_tag="KPD58_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0267179.1"
                     /translation="MKIIDLLDEKSIKLNLNSKTKSEAIEELVDLVANSGNLNDKENY
                     KKAILAREEMSTTGIGEGVAIPHAKNSSVTKACIAAAVSKEGIDYESFDGSLSNLFFM
                     IAAPDGANNTHLEVLSRLSTILMDEDFRNKLINSSSEKEFKEIIDKKEREKFSEEYQD
                     EKVTEKNIIDTKENDANKYPKVLAVTACPTGIAHTFMAAESLNKMAENKGVSIKVETN
                     GSAGVKNKLTKDEIENATCIIVAADKNVEMARFNGKKVIKTKVADGIHKAEELIDKAV
                     NGDAPIYHGGDGSHNESNEESESGFRKVYKHLMNGVSNMLPFVIGGGILIAIAFLLDD
                     YTINPSNFGSNTPIAAFFKGIGDKAFGFMLPVLAGYIAYSISDRPAFVVGFVGGALAG
                     DGGSGFLGALLAGFIAGYLVEGLKKIFSVLPASLEGIKPVLLYPLLGTLLMGIIMTFL
                     VIPPVTAINNAMVGFLNGLGGTSKIVLGLVLGGMMAVDMGGPVNKAAYVFGVASLESG
                     QFEIMAAVMAGGMVPPLAIALATTFFKNRFTKEERDSGKVNYVMGLSFVTEGAIPFAA
                     GDPLHVIPACVGGSAVAGALSMLFNAALRAPHGGVFVIPVVTHPFAYILAIAVGALVG
                     MMLLALLKKPLNQEV"
BASE COUNT         1253 a          351 c          637 g         1017 t
ORIGIN      
        1 gataatataa aactaaataa gataaaaacg ataaatttct cttttaatta agagaaattt
       61 tttgactttc aaggatatta atataaagtt aagatataaa cataaagcaa ataaataaaa
      121 aagaggtggc aatatgattt atacagtgac attaaatcca tcaatagatt atatagttaa
      181 gctagatgag ctaaagacag gaagtacaaa tcgtgtaaaa gaagaatacg tttatccagg
      241 aggaaaaggt ataaatgtat ctcgcatact taaggaactt ggaaatgata atacatcttt
      301 aggttttata agtggtttta ctggagaata tataataaga acattggaag aaaaagaatt
      361 gaagacagat tttataaaga taaaaagtgg tttttcaaga ataaatgtaa aaataaaggc
      421 gttaaaggaa actgagatta atggacaagg tccaaatata gatgatgaag acattgatac
      481 tttatataaa aaacttgata agctaaatca agatgatatt ttaatattgg caggaagtat
      541 accatctact ttagatgaaa aactatacga aaatataatg gctagattag aaaaaaagaa
      601 tataaaagtt gtagtagatg caactaaaaa tcttcttctt aatgttttaa aatataaacc
      661 ttttctaatt aagcctaata acgacgaatt agaagaacta tttggtgtta aattaaattc
      721 aattgaagac atggtaaaat atgctagaag gttaaaggaa atgggagcta taaatgtctt
      781 ggtatcaatg ggaaaagatg gagcattgtt gattacagag gaagatgaag tgcttataag
      841 tgatgttcca aaaggaaaag taaaaaattc tgtaggagca ggtgattcaa tggtagcagg
      901 atttatatct gggtatttga atactgggaa atatgattat gctcttaaat tgggagcagc
      961 ttcaggtagt gcaactgctt tttcatatga cttagccaaa agagaataca ttgataaatt
     1021 ggtaaatgag atatctgtaa agcagtttta aaatattaaa aaatatatag aaaggtaatt
     1081 aatatttatg aaaattatag atttattaga tgaaaagtca attaagttaa atttaaattc
     1141 aaaaaccaaa tcagaagcaa tagaagaatt agtagatttg gtcgcaaata gtggaaattt
     1201 aaatgataaa gaaaactaca aaaaagcaat tttggctcgt gaagaaatga gtacaacagg
     1261 aattggagaa ggtgtagcta tacctcatgc taaaaattct tcagttacta aagcttgtat
     1321 agcagcagca gtgtcaaaag aaggtataga ttatgaatcg tttgacggaa gtttatcaaa
     1381 tctatttttt atgatagctg caccagacgg agctaacaat actcatcttg aagtattatc
     1441 aagattatct acaatactta tggatgaaga ctttagaaat aaacttataa attcaagctc
     1501 tgaaaaagaa tttaaggaaa taatagataa aaaggaaaga gaaaaattca gtgaagaata
     1561 tcaagatgag aaagttactg aaaaaaatat aatagatact aaagagaatg atgctaataa
     1621 gtatcctaag gttttagcag taacagcatg tccaactgga atagcacata catttatggc
     1681 agctgaaagt ttaaataaaa tggcagaaaa caaaggtgta tcaataaaag tagaaactaa
     1741 tggttcagca ggtgttaaaa ataagcttac aaaagatgaa atagaaaatg caacatgtat
     1801 aatagtggcg gcagataaaa atgttgagat ggctagattt aatggtaaaa aagtcataaa
     1861 aactaaggtg gctgatggta tacacaaagc ggaagaactt atagacaagg ctgtaaatgg
     1921 agatgctcca atttaccatg gaggagatgg aagtcacaac gaatcaaatg aagaaagtga
     1981 aagtggattt agaaaagtat acaaacactt aatgaatggt gtatctaata tgttaccatt
     2041 tgttattggt ggagggatac ttatagcgat agccttttta cttgatgatt atactattaa
     2101 cccttcaaac tttggttcaa atacacctat agcagcattt tttaaaggaa taggagataa
     2161 agcttttgga tttatgttac ctgtacttgc tggatatata gcatactcta tatctgatag
     2221 accagcgttt gttgtaggat ttgtaggagg tgctctagca ggtgatggtg gttcaggttt
     2281 tttaggtgca ctacttgcag gttttatagc aggttattta gtagaagggc ttaagaaaat
     2341 atttagtgta ctaccagctt cattggaagg aataaaacca gtattgttat accctttact
     2401 tggaacactt ttgatgggaa ttataatgac atttttagta atacctccag ttacagcaat
     2461 taataatgca atggttggat ttttaaatgg tttaggtgga acttcaaaaa tagttttagg
     2521 attagtatta ggtggaatga tggctgtgga tatgggtgga cctgtaaata aagcagcata
     2581 tgtatttgga gttgcatcac ttgaatcagg gcagtttgaa ataatggcag cagtaatggc
     2641 tggtggtatg gttccgccac ttgctatagc tcttgcaact acatttttta agaatagatt
     2701 tactaaagaa gaaagagatt ctggtaaagt taattatgta atgggattat catttgttac
     2761 tgaaggagct ataccttttg cagctggaga cccacttcat gttataccag cttgtgttgg
     2821 aggttcagca gtggcaggag cacttagtat gttgttcaac gcagctcttc gtgcacctca
     2881 tggaggagta tttgttatac cagtggttac ccatccattt gcatatatat tagctatagc
     2941 tgtaggtgct ttggttggaa tgatgctttt agcattactt aaaaaaccat taaatcaaga
     3001 agtatagcaa ttaaaggata ttcaagaact taaaatatga tgttttataa aaattaatat
     3061 attgctgaat tattttaaaa taaggatttt atgaggctgt tatataacga tttgtaattg
     3121 ttatcttgac agtcttttat ttttatataa gtaaaataag tgtgttttgc tttatttgta
     3181 tttaaattgt ttttaagtta gaatataaag aaaattatat attattttaa tattcactcg
     3241 aactagaaaa taatattt
//
LOCUS       DAELLV010000309         3225 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.309, whole genome shotgun sequence.
ACCESSION   DAELLV010000309 DAELLV010000000
VERSION     DAELLV010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.309"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            535..729
                     /locus_tag="KPD58_003454"
     CDS             535..729
                     /locus_tag="KPD58_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HBG0267180.1"
                     /translation="MSVIVMCSRCGKEIDKYRNKYITYYEKNTNAEIKVCINCALDLL
                     EKTRENQELNIQEFQDTIIL"
     gene            complement(831..1340)
                     /gene="vanZ1"
                     /locus_tag="KPD58_003455"
     CDS             complement(831..1340)
                     /gene="vanZ1"
                     /locus_tag="KPD58_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ1"
                     /protein_id="HBG0267181.1"
                     /translation="MKSRKHNITKGLFIVYIIILTWIILFKLQFDISSLETMNLRSIN
                     LIPFAGSLIINNRVDISEIILNVAIFVPFGIYVCMLKEEWSFIKKVIPIFITSLVFET
                     LQYIFALGASDITDLIGNTLGGIIGIAVFMLLSKIFKNNTIKIINVLALIVTIIVVLF
                     LGLVIFANL"
     gene            complement(1660..2049)
                     /locus_tag="KPD58_003456"
     CDS             complement(1660..2049)
                     /locus_tag="KPD58_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0267182.1"
                     /translation="MKINKLPHAELKLMKYIWGVDDVLASRDIIEDMKLKYDWKKSTT
                     LTFLKNLVDKGFLTTDKVDRCTHYTIAIKEKDYLKVETKSFFSFMHNNSFKSFISALH
                     DDEVLDSKSLDKLEEYFKNLKEEDIDD"
     gene            2442..2798
                     /locus_tag="KPD58_003457"
                     /pseudo
     CDS             2442..2798
                     /locus_tag="KPD58_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889008.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1192 a          401 c          333 g         1299 t
ORIGIN      
        1 cactactctt tatatgtaat tttctactat tatatattta tttactctat aaaataatcc
       61 ccattcaaaa cacttttctt aaatcactca aactaaaata catataagat tatcattagt
      121 cagtaagtat atatttttct aatttctaaa aatacatcca aatcatcaaa cgtttttagc
      181 aattacttta ttattataca gtgttaaagt aaagttccta aataaattgt tattcatata
      241 gcaaataaat acttggtatc aattttttaa tatctattta aatagtattt tttattttgt
      301 cactttatag atattaatgt gtatttaaca ctgctaaaaa ataataaaat tttataatct
      361 attcaataat atactttttt ctataaatat tttacccata acctttattt ttttattaaa
      421 atgcattata ataaaaagta aatctaaatt tgatacagtt ttaacaatca ttattttcag
      481 aaaagtgata ctattgaata tattaagaat aattagagat ggaggaacta agatatgagc
      541 gtaattgtaa tgtgtagtcg ttgtggaaaa gaaatagata aatatagaaa taaatatata
      601 acttattatg agaaaaatac caatgcagaa attaaagttt gtataaattg tgcgttagat
      661 ttattagaaa aaacaagaga aaatcaagaa ttgaacatac aagagtttca agatacaata
      721 attctttaat aatttaggaa agcacctgta taggtgcttt ttatgtgtaa ataattttat
      781 atgtaactgt atcttacgtt ctattttaag ctaaacatat atacttattt ctataagttt
      841 gcaaaaataa ctaatcctaa aaataataca acaataattg tcacaatcaa tgccaataca
      901 ttgataattt ttatggtatt attcttaaat attttcgata atagcataaa aacagctatt
      961 cctatgattc ctcctagtgt gtttcctatc aaatccgtta tatcacttgc acctagtgca
     1021 aatatatatt gaagtgtttc aaataccaaa ctagttataa aaataggtat aacttttttt
     1081 ataaagctcc attcttcttt tagcatacac acatatatcc caaatggtac aaatatagcc
     1141 acatttaata taatctctga tatatctact ctattattaa ttataagtga cccagcaaaa
     1201 ggtattaagt ttatgcttct aagattcatg gtttcaagag atgagatatc aaattgcaac
     1261 ttaaatagta ttatccatgt taaaataatt atgtaaacta taaataatcc ttttgtaata
     1321 ttatgttttc tagatttcat ttattacctc cttactatat tattgtatta ttttattaat
     1381 acaataatat agtaaggtaa tttatctgtc aactatttct tctaaaatta tattcctttt
     1441 ataatttcaa tatctttatc atatttacat gatttaagct attgtagtat ttcatatatc
     1501 tttaaatttt aattggtatt attctctata ttttttacta tcaaacacta aggaaataat
     1561 cacttttctt tatataatgt tctaccatca cataaaacta acattaaatc caaacgctac
     1621 tctcaattgt ctcaaactaa aaagatactg atagctttat tagtcatcaa tatcttcttc
     1681 tttcaaattt ttaaagtatt cttctaattt gtccaagctc ttactatcta aaacttcatc
     1741 atcatgtagt gctgaaataa aactcttaaa tgaattgtta tgcataaaac taaaaaaact
     1801 ctttgtctca acttttaagt aatctttctc ttttatagct atagtataat gcgtacatct
     1861 atctacctta tcagtagtta aaaagccctt atctactaaa ttttttaaaa atgtcaatgt
     1921 tgtgctcttt ttccaatcgt atttcaactt catgtcttct atgatatctc ttgaggctaa
     1981 tacatcatct actccccaaa tatacttcat tagttttagt tctgcatgtg gtaatttatt
     2041 aatcttcatt agtttacacc tcttaccaat ttatgtataa ctaaacttta taacaataag
     2101 tgtatgtaat caattagatt ggaataaata taaattccaa tttattctat ataaaaataa
     2161 ttaataaaaa agaagtactc tactttagag agtacttttg gattatatat tcaaaaattt
     2221 atctaacatg attagagtat gttttatgct ttagtgtgat aattttagtt cgttttatta
     2281 ttgcaacatg tattatattt cattataatc tataagctta aatacaggat atttatttca
     2341 tatacttaat ttatattaag ttttattata tgtattatta agttttacta tattttttat
     2401 taatatttaa tatagttaat ttttaataat tatattatac aataacaata tataagctaa
     2461 ttaaaaacct taagaagaac gatattgaca ctacttgaat tatattatag aaaagctgaa
     2521 ataaataaaa tagtttgggc tagaataggt tttaatttat atctttctaa aggagttgca
     2581 ttaactttaa ctgatgctgg tatagggatt tcaggacttt tggctggtgc tattcctggt
     2641 ataggacagt acttgccact tatagttaga ttaattacaa tgtaccttga gtcaaatata
     2701 acatctggaa aaatctttaa atttaaaaaa tacgttataa taaaggaggt gcttgtgtaa
     2761 ccaaatacta cttacaatct gtagaaatca ataggtgata aaatgattat aaaatatcta
     2821 ctttttgcaa ttgtttcagc attaatttta ttattttgta ataaagcatt ccaactaagt
     2881 aaattacaca agttcattat attgtttgtt tcatttttta tactaacttt tgtagcatca
     2941 ttcattagta gataaaaaat aagatggtgg ggttaactta ccatcttttt ctcatttaat
     3001 atttgtaata ttttagatac tctttatact tgtgttactg ttatactttg attaggaaaa
     3061 ttaatacaac atgagttgat gattatatta taaaggaggt atcttctatg aattttagga
     3121 acatttatct tatacttatg ggattgggaa ttataatatt aactacaata atatctttaa
     3181 ttgaaattaa aattggtttt tgtaatgaaa aatattttaa taaat
//
LOCUS       DAELLV010000310         3181 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.310, whole genome shotgun sequence.
ACCESSION   DAELLV010000310 DAELLV010000000
VERSION     DAELLV010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.310"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            214..1035
                     /locus_tag="KPD58_003458"
     CDS             214..1035
                     /locus_tag="KPD58_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HBG0267183.1"
                     /translation="MNLIDNHIHTNFSSDGKDSMEDTIKKAISIGVRYLTFTDHLEHD
                     EDRGFCINYNDYVPVFNKFKEKYKKDIELLLGVEVGYRKHLKNEIEEIINSNPFDFVL
                     CSTHTIDNVPVPSKEYFKGLSKEDAYYKYFNSILETNREFGDYNIYGHLDYISRYGIY
                     SDNRVIYNDFKDVIDEVLKSIINNGSGIELNTSGYRYGLNAIHPNEDILKRYKELGGF
                     IVTVGSDSHRVEDICKDFDVAYDMLKYLDFKYVSLFKERETYFVNIEKVKSNNIA"
     gene            1293..3176
                     /locus_tag="KPD58_003459"
     CDS             1293..3176
                     /locus_tag="KPD58_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HBG0267184.1"
                     /translation="MSNKNKENITLSNQIKGTIQNSLTKEAILHIPMSNYAYGYDRET
                     LHIRLRTKKNEAKRVILRIGDQYVWDKGGAGGGNLNASGVGWSGGTNIVMSKEVETEL
                     FDYWIAKCKPLNKRSRYGFIIEGQEEKLLFTEKRIIELGNENDEKELCEIGNFFGYPY
                     LNYIDIPKVPNWVKDTVWYQIFPDRFANGDPSINPEGVDKWGAIPTRDNFTGGDLQGV
                     IEHLDYLSDLGINGIYFCPITIGKTNHRYDTVDYMEVDPTLGDKETLKKLIEEAHKRN
                     IRIMLDAVFNHIGYYSKQWQDVVQNKENSRYKNWFYIKDMSKIDTPIEQIDEKNIPYE
                     TFGCEKYMPKLNTENSEVIKYLLDVGKYWIQEFDIDAWRLDVSNEVDHVFWRKFREEV
                     KKVKPDIYILGEIWHGSLPWLMGDQFDSVMNYLMSEAMKKFFCTDEINAEEFKYMIND
                     VMVSYPIQVSEVIFNLLGSHDTTRILTYANGNIDRFKLAYMFMFVQTGCPCIYYGDEI
                     GMEGELSSISEGQRKCMKWNEEKWNKDIFDFMKKIIRLRKENKELRSISNEWVLADKE
                     NGTIILKKESISIIINNSSKNIILTLPDYLANKKVKDLYEEEIVDLKEELMLEKYKFI
                     ILK"
BASE COUNT         1312 a          276 c          567 g         1026 t
ORIGIN      
        1 aaattaagac ttttaaaaat taaattgaag tatataaaag taacattaag agaagtatcc
       61 ttaatgattt tctaaaatca gaaataggtg cttctctttt ttgtaaaaat aaaagtatgt
      121 aaataaaatt taaccattat aattttcttt taacaaatgt atacaataaa cttgttgtat
      181 aattaagata gataaaataa agggagggat aatatgaatt taatagataa tcatattcat
      241 actaattttt caagtgatgg aaaggattcc atggaagata caataaaaaa agctataagt
      301 attggtgtta gatatttgac ttttacagac catttagaac atgatgaaga tagaggattt
      361 tgcataaatt ataatgacta tgttccagta tttaataagt ttaaagaaaa atataaaaag
      421 gacatagagt tattactagg tgttgaagtg ggttacagaa aacatctaaa aaatgaaata
      481 gaagaaataa taaattctaa tccttttgat tttgtattgt gctcaactca tacaattgac
      541 aatgtacctg ttccatcaaa ggagtatttt aaaggtctta gcaaagaaga tgcgtattat
      601 aaatatttta atagcatact tgaaactaat cgtgaatttg gagattacaa tatatatggt
      661 catttggatt atatatctag gtatggaata tattcagata atagagttat atacaatgac
      721 tttaaagatg ttattgatga ggtcttaaag tcaataatca ataatgggag tggtatagag
      781 ctaaatactt ctggttatag atatggacta aatgctatac atccaaatga agatattcta
      841 aaaagatata aagaacttgg aggatttata gttactgtag gttctgattc tcatagggta
      901 gaagatatat gcaaagattt tgatgtggcc tatgatatgc ttaagtatct tgactttaaa
      961 tatgtatctt tatttaaaga aagagaaact tattttgtaa atatagaaaa agttaaatct
     1021 aacaatatag cttagagtgt gttttgaagt aaattaatgg atagttaaaa agttaatagt
     1081 tataatattg tatcctctag aaaaatacta gaggttttta ttttgctaaa ataactaaat
     1141 agaattaaat ttaaatagta tagtttattt taaattgaat agtttattgt tgatatattt
     1201 tactattata tatttttatt gtgatataat agtaaaatat attaacctat ctttagatta
     1261 tataaattat tataaaggag ggaaatttta aaatgtctaa taaaaataaa gaaaatataa
     1321 ctttaagtaa tcaaatcaaa ggaacaattc aaaatagttt aacaaaagaa gctatattac
     1381 atataccaat gagcaattat gcctatggtt atgatagaga aacactacat ataagactta
     1441 gaactaaaaa aaatgaagcg aaaagagtaa ttttacgaat aggagaccaa tatgtgtggg
     1501 ataaaggagg tgcaggagga ggaaatttaa atgcatctgg agttggttgg tctggtggaa
     1561 caaatatagt aatgagtaaa gaagtagaaa ctgaattatt tgactattgg atagctaagt
     1621 gtaagccttt aaataaacgc tcacgatatg gttttataat agaaggtcaa gaggaaaaac
     1681 ttttatttac agaaaaaaga ataatagagt tgggaaatga aaatgatgaa aaggaattat
     1741 gtgaaatagg taattttttt ggatatccat atttaaatta tatagatata cctaaagtgc
     1801 caaattgggt gaaagatact gtttggtatc agatatttcc agatagattt gcaaatggtg
     1861 acccgtctat aaacccagaa ggagtagata aatggggagc tattccaacc agagataatt
     1921 ttacaggtgg agatttacaa ggtgtgatag aacacttaga ttatttgagt gatttgggaa
     1981 taaacggaat ttacttttgc cctattacaa ttggaaaaac caatcataga tatgatacgg
     2041 tagattatat ggaggtagac cctacattag gagataaaga gactttaaaa aaactaatag
     2101 aagaagcgca taagagaaat ataagaataa tgttggatgc tgtttttaat catataggat
     2161 attattctaa acagtggcaa gatgtagttc aaaataagga aaattctcga tataaaaact
     2221 ggttttatat aaaagatatg agtaaaattg atactccaat agaacaaata gatgaaaaaa
     2281 atatacctta tgaaaccttt gggtgtgaaa aatatatgcc taaacttaat acagaaaatt
     2341 cagaagtaat aaaatattta ttggatgtag gcaagtactg gatacaggaa tttgatatag
     2401 atgcgtggag attagatgtt tcaaatgaag tagaccatgt tttttggaga aagtttagag
     2461 aagaagtcaa aaaagtaaaa ccagacattt atatattggg agaaatttgg catggtagtt
     2521 taccatggct aatgggtgac caatttgatt cagttatgaa ttacttgatg tcagaagcca
     2581 tgaaaaaatt cttttgtaca gatgagataa acgccgaaga atttaagtat atgataaatg
     2641 atgtaatggt aagttatcca atacaagtta gtgaagttat ttttaattta cttggaagtc
     2701 acgatactac aagaatattg acttatgcta atggaaatat tgatagattt aaacttgcat
     2761 atatgtttat gtttgtacaa acaggatgtc cttgtatata ctatggtgat gaaatcggaa
     2821 tggaaggaga attaagttca atatctgaag gtcaaagaaa atgtatgaaa tggaatgaag
     2881 aaaaatggaa taaggatata tttgatttta tgaagaagat tattagactt cgtaaagaaa
     2941 ataaagaact taggtctata tcaaatgaat gggtgttagc agataaggaa aatggaacga
     3001 ttatattgaa aaaagaatct atttctataa taataaataa ttcatctaaa aatataattt
     3061 tgacgttacc agattattta gcaaataaaa aagtaaaaga tttatatgaa gaagaaatag
     3121 ttgatttaaa agaagaacta atgttagaga aatacaagtt cattatttta aaataataca
     3181 a
//
LOCUS       DAELLV010000311         3181 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.311, whole genome shotgun sequence.
ACCESSION   DAELLV010000311 DAELLV010000000
VERSION     DAELLV010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.311"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            160..1665
                     /locus_tag="KPD58_003460"
     CDS             160..1665
                     /locus_tag="KPD58_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HBG0267185.1"
                     /translation="MKVGFDHKKYLEEQSKYILERVNNFDKLYLEFGGKLMGDLHAKR
                     VLPGFDENAKIKVLQHMKEKVEVVICVYAGDIERNKIRGDFGITYDMEVLRLIDDLRG
                     YELDVNSVVITRFEGQPATTVFINKLERRGIKVYKHYPTKGYPSDVETIVSDEGYGAN
                     EYIETTKPIVVVTAPGPNSGKLGTCLSQLYHENKRGNVVGYSKFETFPVWNVPVKHPL
                     NIAYEAATVDLKDVNMIDSFHLETYGEMSVNYNRDLELFPVLKKIIEKITGKESIFQS
                     PTDMGVNRVGFGIIDDEVIRKASIEEIIRRYFKTACEYKKGQVDKGAYDRMKMIMEEL
                     NLKPEDRNVVLPARNYSNKLKESADKNDTCPVVALELEDGTILTGKGSDLMNGTAAVV
                     LNAIKHLANISDDMHLISPVILEPIINLKTKTLASRNVSLSCQEVLTALSICAVTNPT
                     AQFAMEKLALLKGCQAHSTTILNRDDEQTFRKLGIDVTCDPEYPSQNLYYS"
     gene            2055..3152
                     /gene="dinB"
                     /locus_tag="KPD58_003461"
     CDS             2055..3152
                     /gene="dinB"
                     /locus_tag="KPD58_003461"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBG0267186.1"
                     /translation="MKHYRKIIHIDMDAFYASIEQRDNKKLKGRPVIVGGNPQSRGVV
                     ATCSYEARKFGIHSAMPSAVAYNRCPYAVFVRPRFDVYKSVSEQIRDIFYRYTDLVEP
                     LSLDEAYLDVTKNKKNIDSSIEIAKQIKKDIFREVGLTSSAGVSYNKFLAKIASDLRK
                     PNGLTVITEENAQDFLDKLPVNKFFGVGKVTSNTLKNLGIKTGYDLRCLNLFELENIF
                     KKRGYELYKFARGIDDRPVEPNRVRKSVGAETTLSHNLDIDEEETRNILDELCEEVCH
                     RLKNSEKFGKTLTLKIKYEDFTKITRSLSLEHYIDEYNDIRSGVDNLLRNVEVNGKQI
                     RLLGVTISNLSDKKETYKDITLFEYMDSIQM"
BASE COUNT         1273 a          338 c          566 g         1004 t
ORIGIN      
        1 cttatttacc ataaaactat cttgaaaaaa attaatctta attgtataat attactttga
       61 gtgaaaaagt attaacattt ttttataaga tgttttacta aaaaaatata tataaatata
      121 ctgttatcaa taagaaattt aatttagtga ggtgttaaga tgaaagtagg atttgaccac
      181 aaaaaatatt tagaagagca gtctaaatat atcttagaaa gagtaaataa ttttgacaaa
      241 ctttatttag agtttggagg aaaattgatg ggagaccttc atgcaaaaag agtacttcca
      301 ggatttgatg aaaatgcaaa gataaaagta ttacaacata tgaaagaaaa agttgaagtt
      361 gtaatttgtg tctatgcagg agatatagaa agaaacaaaa taagaggaga ctttggtatc
      421 acttatgata tggaagtttt aagattaata gatgatttaa gaggttatga actagatgta
      481 aatagtgtag taattactag atttgaagga caacctgcta caactgtatt tataaataaa
      541 ttagaacgta gaggaattaa agtttataag cattatccaa caaagggata tccgtcagat
      601 gttgagacta tagttagtga tgagggatat ggagcaaatg aatatataga aactacaaag
      661 ccaatagtag tagtcacagc accaggacct aatagtggaa aacttggtac ttgtctaagt
      721 cagttgtatc atgaaaataa aagaggtaat gtagttggat attctaagtt tgagacattc
      781 ccagtttgga atgtacctgt aaaacatcct ttgaatatag cttatgaagc agctacagtt
      841 gatttaaaag atgtgaatat gatagattca ttccaccttg aaacgtatgg tgaaatgtca
      901 gtaaactaca atagagattt ggaattattt ccagtgctta aaaaaataat agaaaagata
      961 actggaaaag aatcaatttt ccaatctcca actgatatgg gtgttaatag agttggcttt
     1021 ggaataatag atgatgaagt aataagaaaa gcttctatag aagaaataat aagaagatac
     1081 tttaaaactg cctgtgagta taaaaaggga caagttgata aaggtgctta tgatagaatg
     1141 aaaatgatta tggaagagct taacttaaag ccagaagata gaaatgttgt attacctgct
     1201 agaaactaca gtaataaatt gaaagaaagt gccgacaaga atgatacttg tcctgtagtt
     1261 gcattagaat tagaagatgg tacaatatta actggtaagg gttctgattt aatgaatgga
     1321 accgcagcag tagtattaaa tgccattaaa catttagcca atatatctga tgatatgcac
     1381 ttaatatcac ctgtaatatt agaacctatc ataaacttaa aaacaaagac tttagctagt
     1441 agaaatgttt cacttagttg ccaagaggtg ttaactgcac ttagtatttg tgctgtaact
     1501 aatccaactg cacaatttgc tatggaaaag ttagcattat taaaagggtg tcaagctcat
     1561 tctactacta tattaaatag agatgatgaa caaacattta gaaaacttgg tatagatgta
     1621 acttgtgacc cagaatatcc ttcacaaaat ctttactata gttaaaatta tataaaaaaa
     1681 tatgggctgt tttaaagtaa tagtttgagc agtccattac aattataata aaatcagtag
     1741 gctgtcttgt ataagacagt cttttgtttt taaaacatcc cataaattaa atatatatac
     1801 tgttatccat aaggaatttt aaatttagtt ccttggaata cctattgtaa agcttttata
     1861 tagtttttgc aagtgttttt aaggattttt tataaattat attgaagtta atggattata
     1921 gatggtttta tttatggaaa atatgaatat ataaagaaaa taatgaaaaa ataccaaaaa
     1981 gaaacataag tttggtataa ttaaaaagta atataaataa aattaatcta tttatacttt
     2041 aaaaggtgat aataatgaaa cattatagaa aaataattca tatagatatg gacgcttttt
     2101 atgcatctat agaacaaaga gataataaaa aattaaaagg aagacctgtt attgtcggtg
     2161 gaaatccaca aagccgaggt gtggttgcaa cgtgttctta tgaagctaga aaatttggta
     2221 tccattcagc tatgccatca gctgtagctt acaataggtg tccatatgca gtttttgtaa
     2281 gacctagatt tgatgtttat aagagtgtgt cagagcaaat aagagatata ttttatagat
     2341 acacagattt agttgaacca ttatctttag atgaagctta cttagatgta acaaaaaata
     2401 aaaaaaatat tgattcatca attgagatag caaagcaaat aaaaaaagat atatttagag
     2461 aagttggtct tacttcttca gctggtgtgt catacaataa gtttcttgct aagattgcat
     2521 cagacttaag aaagccaaat ggattaactg taataaccga agaaaatgcc caggactttt
     2581 tagataaatt acctgtgaat aaattttttg gtgttggaaa agttacttca aatactctta
     2641 aaaatttagg tataaaaact ggatatgatt taaggtgttt aaatttattt gaacttgaaa
     2701 atatattcaa aaaaagaggt tatgaattat ataaatttgc tagaggaata gacgatagac
     2761 cagtggaacc aaatagggtt agaaaatctg ttggggctga gactacatta agtcataatt
     2821 tggatattga tgaagaagaa acacgcaaca ttcttgatga actttgtgaa gaagtatgtc
     2881 atagattaaa aaactctgag aagtttggaa aaacattaac attaaaaatt aaatatgagg
     2941 actttacaaa gataactaga agccttagtt tagaacacta tattgacgaa tataatgata
     3001 taagaagtgg tgtagataat ttgttgagaa atgttgaggt taatggaaaa caaattaggc
     3061 tattgggagt tacaatatca aacctaagtg ataaaaaaga aacttataaa gatattacat
     3121 tatttgaata catggatagt atccaaatgt gagttttgtt taaatgaaat aacactagct
     3181 t
//
LOCUS       DAELLV010000312         3174 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.312, whole genome shotgun sequence.
ACCESSION   DAELLV010000312 DAELLV010000000
VERSION     DAELLV010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.312"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            145..825
                     /gene="pgmB"
                     /locus_tag="KPD58_003462"
     CDS             145..825
                     /gene="pgmB"
                     /locus_tag="KPD58_003462"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBG0267187.1"
                     /translation="MIEAFIFDLDGVITDTAYYHYMAWRKLAHKVGIDIDTKFNESLK
                     GISRMESLDRILEFGNKKYSFSEEEKVRMAEEKNNYYVSLIDEITSNDILPGIESLLI
                     DVKSNNIKIGLSSASKNAINVLNHLGISDKFDFIADAGKCKNNKPHPEIFLMSAKGLN
                     VNPQNCIGIEDASAGIDAINSANMFSVGVGNYENLKKANLVVDSTNQLKFEYIQEKYN
                     EYIVRRII"
     gene            822..3107
                     /locus_tag="KPD58_003463"
     CDS             822..3107
                     /locus_tag="KPD58_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBG0267188.1"
                     /translation="MKCLFSNVDEWKIIQDKIEIKENRLAESIMSIGNGYMGMRGNYE
                     ENYSGDTHKGSYIAGVWFPDKTRVGWWKNGYPEYFGKIPNSVNYIGVRIFIDDVELDL
                     AKCKVEDFYRELDMKNGILNRRFIVNINDKKFEVRVARFLSISVKELAVIKYSIKSLN
                     SNTKIKFVPYLDSNIKNEDSNYEEKFWNGISSSSLEYKGSILSRTKENAFNTPVFTVG
                     SMMYIKTNGDVEAISHESDYNYCENSITMDVLKNEVASIEKYVSITSTRDYKESELLR
                     KCEDILNRELSKSYDDILNEHTKLWNKRWESADVKINGDKSSQQGIRYNLFQLFSTYY
                     GEDSRLNIGPKGFTGEKYGGATYWDTEAFCVPVYLGVADKEVTRNLLEYRYNQLEQAK
                     DNAKKLGLKGALYPMVTFDGVECHNEWEITFEEIHRNGSIVYAIYNYTNYTGDYKYIK
                     EKGIDVIIEIARFWASRVHLSTKKDLYMIHGVTGPNEYENNINNNWYTNYIAKWCLDY
                     AVENILRLEKECNECIDRNNVTREEVEFWKIISNKMYLPYDEELQIIIQNDNFLDKEF
                     VEVKDLPKENFPLNQNWSWDKILRSCFIKQADVLQGIYYFGNRFTKEEKKRNFDFYEK
                     YTVHESSLSSSIYSIIASEIDNLEKAYELYSRTARLDLDNYNNDTNDGLHITSMSGSW
                     LSIVHGFAGMRTWNETLSFDPKLPKEWKNYSFNINYRDNIINVDVCSDLIKIENLKGR
                     PIKIIVYGKKYILEKCIKIDK"
BASE COUNT         1306 a          279 c          589 g         1000 t
ORIGIN      
        1 ggtctacaag tgagtgaatt gaaagtagat tttaaattaa agactgagtt agagtagata
       61 attttataaa taatatctta aatacaagat agaaaataaa ttgattatta gtctaaaaat
      121 ttcttgtaga aagaggaaat aagaatgatt gaagcattta tatttgattt agatggtgta
      181 attacagata ctgcatatta tcactatatg gcttggagaa aactagccca taaagttggg
      241 atagatatag acacaaaatt taatgaatct ttaaaaggca taagtagaat ggagtcattg
      301 gacaggatat tagaatttgg aaataagaaa tattcatttt cagaagaaga aaaagtgaga
      361 atggctgaag aaaaaaataa ttactatgta tcattgatag atgagataac ttcaaatgat
      421 attttgccag gtatagaaag cttgttaatt gatgtaaaat ctaataatat aaagataggt
      481 ctatcttctg caagtaagaa tgcaataaat gtattaaatc atttgggtat aagtgataaa
      541 tttgatttta tagctgatgc aggaaaatgt aaaaacaata aaccacatcc agagatattt
      601 cttatgtcag cgaaagggct taatgtaaat ccacaaaatt gcatagggat agaggatgca
      661 agtgcaggta tagacgctat aaactcagct aatatgtttt ctgttggtgt tggaaattat
      721 gaaaacttaa aaaaagcaaa tttagttgta gattcaacaa atcaattaaa gtttgaatat
      781 atacaagaaa agtataatga gtatatagtt aggagaatta tatgaaatgt ttatttagta
      841 atgtagatga gtggaagatt atacaagata agattgaaat caaagaaaat agattagcag
      901 agtccataat gagtataggc aatggatata tgggtatgag aggaaactat gaggaaaatt
      961 atagtggaga tacacacaag gggtcttata tagctggagt atggtttcca gataaaactc
     1021 gtgttggttg gtggaaaaat gggtatccag aatactttgg taaaattcct aattcagtca
     1081 actatatagg tgtaagaatt ttcatagatg atgtagaatt agatttagca aaatgtaaag
     1141 tagaagattt ttatagagaa ttagatatga aaaatggtat tttaaatcgt aggtttattg
     1201 taaatataaa tgataaaaaa tttgaagtta gggttgctag gtttttaagt atatctgtta
     1261 aggaacttgc agtaattaaa tacagtataa aatcattaaa ttctaatact aaaataaaat
     1321 ttgtaccata tttagattca aatataaaaa atgaagattc aaactatgag gaaaagtttt
     1381 ggaatggaat ttcttcaagt tctttagaat ataaagggag tattctttct cgaacaaagg
     1441 agaatgcatt taatacacca gtattcactg tgggttcaat gatgtatatt aagacaaatg
     1501 gtgatgtaga agctatatct catgagagtg attataatta ctgtgaaaat tcgataacaa
     1561 tggatgtttt aaaaaatgag gttgcaagta tagaaaaata tgtatctata acttcaacta
     1621 gagattataa ggaaagtgag ctattaagaa aatgtgaaga cattttaaat agagaactat
     1681 ctaaaagtta tgatgatata ttaaatgaac atacaaagtt atggaataaa agatgggaaa
     1741 gtgcagatgt aaaaataaat ggagataaaa gtagccaaca aggtataaga tataatttat
     1801 tccaactatt ttcaacatat tatggagaag attcgcgatt aaatataggt ccaaaaggat
     1861 ttactggtga aaagtatgga ggagctactt attgggatac agaagcattt tgtgttccag
     1921 tatatctagg tgtagcagat aaggaggtaa ctagaaatct tttagaatat aggtacaatc
     1981 agttagaaca ggcaaaggat aatgcaaaaa aactagggct taaaggtgct ttatatccaa
     2041 tggtaacatt tgatggagta gaatgtcata atgaatggga gataacattt gaggaaatcc
     2101 accgaaatgg aagtatagta tatgcaatat ataactatac aaattataca ggtgactata
     2161 aatatataaa agaaaaagga atagatgtaa taatagaaat agctagattt tgggcaagta
     2221 gagttcattt gtctacaaaa aaagatttgt atatgataca tggagtaaca ggtccaaacg
     2281 agtatgaaaa caatatcaat aataattggt atactaatta tatagctaaa tggtgtttag
     2341 attatgctgt agaaaatatc ttacgattag agaaagagtg taatgaatgt attgacagaa
     2401 ataatgtgac tagggaagaa gttgagtttt ggaaaataat atcaaataaa atgtatctgc
     2461 cgtatgatga ggaactacaa ataataattc aaaatgataa ttttttagat aaagaatttg
     2521 tagaagtaaa ggatttacca aaagaaaatt ttcctcttaa tcaaaattgg tcttgggata
     2581 agattcttag gtcgtgtttt ataaaacaag cagatgtatt acagggaatt tactattttg
     2641 gaaatagatt tactaaagaa gaaaaaaaga gaaattttga cttctatgaa aaatatactg
     2701 tacatgaatc ttctttatct tctagtattt attctattat tgcctcagaa atagataacc
     2761 tagaaaaggc ttatgaatta tattcaagaa cagctaggct tgatttagat aattacaata
     2821 atgatacaaa tgatggactg cacataactt caatgtctgg ttcatggctt tccattgtac
     2881 atggatttgc aggcatgaga acatggaatg aaactctatc ttttgaccct aagttgccaa
     2941 aagagtggaa aaattattct ttcaatataa attatagaga taatattatt aatgtagatg
     3001 tatgtagtga tttaattaaa atagaaaatt taaaaggtag accaataaaa attattgtat
     3061 atgggaaaaa atatatatta gaaaaatgta taaaaattga taaatagatt taataatatt
     3121 tttattatat aaatagttaa aataagctat ctaattattt atttatttta acta
//
LOCUS       DAELLV010000313         3152 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.313, whole genome shotgun sequence.
ACCESSION   DAELLV010000313 DAELLV010000000
VERSION     DAELLV010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.313"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..692)
                     /locus_tag="KPD58_003464"
     CDS             complement(<1..692)
                     /locus_tag="KPD58_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBG0267189.1"
                     /translation="MKYKLFIDFEFNILDDNKNKPKEYNGAELISIGGVLVDNEFNTI
                     DNYYSLVKPKYNKILSNKCKNLTKLNQLDINNAPNLLYVMDDFYKWFCKFDDVTIYNW
                     GDFDITGLLTSFRVYKYTGKCLELFNMMIDIQPFISQHITYNNRILSKQISLLNMKKI
                     FSVEGDIKHNSLSDAVDLMNVCKCFFFNSPKNIDILEELYNKLPALKTPLYYIPYFED
                     ENFELKFDRTPE"
     gene            complement(732..1079)
                     /locus_tag="KPD58_003465"
     CDS             complement(732..1079)
                     /locus_tag="KPD58_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0267190.1"
                     /translation="MLNLKEIREEIIKKYIGDSLISELTEELKNILITDLQSNPFKSN
                     IELSIIPANIEIFKKIENILIDSKVNLKMGWERILINYILKDLFELNYEINKSCNEPL
                     MFRVIISIDSYDA"
     gene            complement(1297..2253)
                     /locus_tag="KPD58_003466"
     CDS             complement(1297..2253)
                     /locus_tag="KPD58_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBG0267191.1"
                     /translation="MKKILITGALGQIGSELTIKLRNEYGEQNVIASSRRVKEGNPVC
                     ESGIFEILDVTDKNRFFEIAKKYDVDTIIHLASLLSAVAESKPLEAWNLNMNGLINGL
                     EIAKELDCKFFTPSSIAAFGENSPKNMTPQDTLQRPNTMYGVTKVSGELLCDYYHSKF
                     GVDTRGVRFPGLISYVTPPGGGTTDYAVDIYYEALKNKRYKSYIAEGTKMDMMYMPDA
                     LQSIVDLIEAPADKLIHRNAFNITAMSFSPEEIADSIKKYIPDFVIEYDVDPVRQSIA
                     DSWPNSLDSSSAVKEWNFKFSYDLDKMTKDMLEKLSEKGIGK"
     gene            2696..3064
                     /locus_tag="KPD58_003467"
     CDS             2696..3064
                     /locus_tag="KPD58_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HBG0267192.1"
                     /translation="MTRPSIYHSQGYTCAEALIKSYNEEHNTDIPISIGSGMGVGMNV
                     GSVCGAVNAAVVIIGYLNGRNSNLDENIARKYSRELLNRVRNKYSSEICVSLKKNKVT
                     CEEIINFTYDALNDMLKNQK"
BASE COUNT         1136 a          423 c          402 g         1191 t
ORIGIN      
        1 tcttctggag ttctatcaaa tttgagttca aaattttcat cttcaaagta gggaatataa
       61 tataaaggtg ttttgagagc aggtaattta ttatataatt cttcaagaat atcgatattt
      121 ttaggggagt taaagaaaaa gcatttacat acattcatta aatccacagc atctgaaagt
      181 gagttatgtt taatatctcc ttctacagaa aaaatttttt tcatattcag taaagatatt
      241 tgcttagata agattctatt attgtatgta atatgttgag atataaaagg ttgaatgtca
      301 atcatcatat taaataactc taagcattta cctgtatatt tatacactct aaatgaagtt
      361 agaaggccag ttatatcaaa atcaccccaa ttatatattg taacatcatc aaatttacaa
      421 aaccatttat aaaaatcatc cataacatat aaaagattag gagcattatt gatatctagt
      481 tggttcaatt tggttaaatt tttacattta tttgaaagta ttttattgta ttttggctta
      541 actaaagagt aataattatc tattgtatta aattcgttat caactaaaac tccaccaata
      601 gaaattaatt ccgcaccatt atattctttt ggtttatttt tattatcatc tagtatatta
      661 aattcaaagt ctataaataa cttgtatttc ataaatctaa agcacatcct caggtaaatt
      721 atttattgag cctaagcatc ataagaatca atagatataa tgactctaaa catcaaaggt
      781 tcattacaac ttttatttat ttcataattt aattcaaata aatcttttaa gatataattt
      841 ataagtattc tttcccaacc catttttaaa ttaactttag agtctattaa tatattttct
      901 atctttttaa atatttctat atttgctggt attatactga gctcaatatt acttttaaac
      961 gggttagact gtaaatctgt tataagtata ttttttaatt cctctgtaag ttcagaaatt
     1021 aaactatcac ctatgtattt ttttatgatt tcctctctaa tttcttttaa atttagcaat
     1081 attagtacct gctacttaca tagtagcttt tcctttcctt ttaatactat tattcatttt
     1141 tgaatcttat ttagatatat tttaagatta aaagtcttta gctatataat tttttaccca
     1201 tttttaactt atattaacat attacaggct tatatctcca ccctgtttac attgaggatg
     1261 gagtatattg cctattttga taaaaatgat aggttgctat ttgcctattc ctttttctga
     1321 taatttttct aacatatctt tggtcatttt atctaaatca taactaaact taaagttcca
     1381 ttcttttact gcactactac tgtctagaga gttaggccaa gaatcagcta tactttgtct
     1441 tacagggtct acatcatatt ctatcacaaa atcaggaata tattttttta tagaatcagc
     1501 tatttcttca ggagaaaagc tcatagcagt aatattaaaa gcatttctat ggatgagttt
     1561 atcagctgga gcttcgatta aatccactat tgattgaagt gcatcaggca tatacatcat
     1621 atccattttt gtaccttctg ctatgtaaga tttatatctc ttatttttta atgcctcata
     1681 atatatatca actgcataat ctgtagttcc tccaccagga ggcgtaacat aagaaataag
     1741 acctggaaat ctaacacctc tagtgtctac tccaaatttt gagtgataat aatcacatag
     1801 taattctcca gaaactttag tcacaccata catagtatta ggtctttgta atgtatcttg
     1861 aggagtcata ttttttggag aattttcacc aaaagcagca attgaactag gtgtaaaaaa
     1921 tttacaatcc aattctttgg ctatttcaag accatttatt aatccattca tatttaagtt
     1981 ccaagcttct agaggtttac tttcagcaac tgcagaaagt aaagatgcta gatgaattat
     2041 agtatctaca tcatattttt ttgcaatttc aaaaaaacga ttcttatctg taacatctaa
     2101 tatctcaaat ataccagatt cacatactgg attaccttct tttactctcc ttgaactagc
     2161 gattacattt tgttctccat actcatttct aagctttatt gttaattctg aacctatttg
     2221 tcctaatgca cctgttataa gtattttttt caattttaca acctcctaag ggtttaattt
     2281 atgtagtact acttattttt attttagcaa atcttttgtt taaatacaaa gaattaaaga
     2341 taataatata cgacttaatt gaatgaagtg cagtaagtgc gtaaaaattt aaagctaata
     2401 tataatggtc tattgtataa caataaaaga gtagattaaa ttattatata tagattgtgt
     2461 gactaagtca cagaaaatag agctaattta atatataata aaagcatgtc aagtaaagct
     2521 ttaaattaaa gtaactttat ataaaaagta atataaattg ataaaattat aaatttattt
     2581 atagtgaata taggttggat tttatagata caaaaatagt atatatgata tgtagaccat
     2641 tagcatgttt aaagtagcaa aacatataaa ataaaatttg aaaggaagta taaaaatgac
     2701 aagaccatca atatatcata gccaaggata cacttgtgca gaggcattaa taaagtctta
     2761 taatgaggaa cacaacacag atataccaat ctcaatagga agtggtatgg gggttggaat
     2821 gaatgttgga agtgtttgtg gagctgtaaa tgctgctgtt gttataatag ggtatttaaa
     2881 tgggcgaaat agtaatttag atgaaaatat agcaaggaag tattcaagag agctattgaa
     2941 tagagtaaga aataaataca gctcagaaat atgtgtatct cttaaaaaaa ataaagtaac
     3001 ttgtgaagaa ataataaatt tcacatatga tgctctaaat gatatgctaa aaaatcaaaa
     3061 ataattctaa gaaacaagta ttttacattc gatagaatac ttgttttttt atgatatttt
     3121 taataagtat tagtttatta taatttttaa at
//
LOCUS       DAELLV010000314         3134 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.314, whole genome shotgun sequence.
ACCESSION   DAELLV010000314 DAELLV010000000
VERSION     DAELLV010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.314"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(135..512)
                     /locus_tag="KPD58_003468"
     CDS             complement(135..512)
                     /locus_tag="KPD58_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0267193.1"
                     /translation="MTISKIPQAELKVMKFIWEKNDTVTSKETIEAMERKYGWKQTTT
                     LTLLSRLVKKHFLSAEKIDRYTHYTIVVGHKEYLSVETKDFLSNIHDNSLQSLISALH
                     DDEVVDKDMLDLFESHFKNLEEE"
     gene            complement(699..1670)
                     /locus_tag="KPD58_003469"
     CDS             complement(699..1670)
                     /locus_tag="KPD58_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0267194.1"
                     /translation="MLLRASIGMGIVIFTMAFVQNVYQLLGLRILQGVFTGYATACTT
                     LIATQTDKNHSGWALGTLATASTTGSLIGPTVGGYIESILGLKSTFIITGGLLFVSFI
                     ISILFVVDNFKPKEIKESISIPKEQLNILPNKFLIASIFVTTFITQLALYSIEPIVTI
                     YISQLTNFASNVALIAGLTFSASGLANLLAAQKLGKMSDKIGPQKVLLISLLWAGIIF
                     IPQAFVKTAWQLMLLRFLLGLSVAGLNPSVNSLLKKIAPEEYVGKIFGYNASAQYIGC
                     SSGAFLGGQISAHLGIRTVFFSTSLLLFRNALWMYKFTGLFIKDKTK"
     gene            complement(1704..1901)
                     /locus_tag="KPD58_003470"
     CDS             complement(1704..1901)
                     /locus_tag="KPD58_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HBG0267195.1"
                     /translation="MELWKRNLFVCWIGMFFSSIGMSQIAPILPLYIKQLGVTDVSLI
                     QQYSGIIFGCTFVVAAFFSPI"
     gene            complement(1969..>3134)
                     /locus_tag="KPD58_003471"
     CDS             complement(1969..>3134)
                     /locus_tag="KPD58_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HBG0267196.1"
                     /translation="YIYDETNIIEKCKELKNNILGFDFLYSIKTNPYNKIVKSIAEQG
                     FGADASSTNEVMISSKCGVSKDDIYYSSPGKTEIDIETVLDKCIIIADSITEIELINK
                     ISKKHNQKVKIGIRINPDFTMDDDSAKSSKFGIDIEDMSSVFKMLSMCDYVSVVGIHI
                     HIKSQVLDFEKLGHYYEKCFFIALNLNKSKYINIEFINFGSGLGVAYDNLRENPLNLK
                     ELSKLTQKIAFTNSNTLKSRLLIESGRFIVCKSGNYYVKIVDIKVSRGKKYLIVKNGL
                     NGFMRPIISNLIENITCGVELEGQEPLYTSNHAFSLSVLNNLKEEEYVNVVGNLCTSL
                     DVIANNVKLKKANIGDLISISNAGSYSYSLSPLLFSSYEFPKQYYKRKDGYIE"
BASE COUNT         1094 a          511 c          344 g         1185 t
ORIGIN      
        1 ttattttagg cattttacta attagagaac ctatttaata ttactttatg aatagtgagt
       61 agctgattaa ataataccat ttatggtttt cagccataga ttaattaaat tcttagagtt
      121 ctatcctgat ataattactc ttcttctaaa tttttaaaat gactttcaaa taaatccaac
      181 atatctttat caacaacttc atcatcatgt agtgctgaaa ttaaactctg taaagaattg
      241 tcgtgtatat tgcttaaaaa atcttttgtc tcaacactta aatactcctt atgtccaaca
      301 actattgtat aatgagtgta tctatctatt ttttctgcac ttaaaaaatg ttttttaaca
      361 agtcttgata aaagtgtcaa tgtagttgtt tgtttccaac catattttcg ttccatagct
      421 tcaatagttt cttttgatgt tactgtatca tttttttccc aaataaactt cataactttt
      481 aattctgctt gtggtatttt tgaaattgtc ataaccttac gcccctctca aacttttatt
      541 aaattaatac tatcgcaata tagtttttaa gtcaaagatt ttgtcgataa taatattttc
      601 caatttttac catagggtta ttttttatat aattctatga taaattacat aaaaaatatt
      661 tattttacat cataaaatta gaaatgtaaa tagtatgatt atttagtctt atctttaata
      721 aacaatccag taaatttata catccaaagt gcattcctaa acaacaataa acttgttgaa
      781 aaaaatactg ttctaatacc taaatgtgct gatatttgtc cccctaaaaa tgctcctgat
      841 gaacagccta tatactgggc agatgcatta tatccaaata tttttcccac atattcttct
      901 ggtgcaattt ttttaagcaa gctgttaaca gaaggattaa gccctgcaac tgacaaccct
      961 aataaaaatc tcaacaacat cagttgccat gcagttttta caaaagcttg tggtataaat
     1021 ataatcccag cccataaaag tgaaattaac aatacttttt gaggacctat tttatccgac
     1081 atttttccaa gtttttgagc tgctaataaa tttgctaatc ctgatgcaga aaatgtcaat
     1141 ccagctatta aagctacatt tgatgcaaaa tttgtaagtt gtgatatata tatggttaca
     1201 attggttcta tagagtataa agctagttga gtaatgaatg tggttacaaa aatagatgca
     1261 attaaaaatt tatttggcaa tatatttaat tgttctttag gtatagaaat tgattctttt
     1321 atttctttag gtttaaaatt atctactaca aataaaatag agatgataaa tgatacaaac
     1381 aacaatcctc ctgttataat aaatgtactt ttaagaccta agattgattc tatatatcct
     1441 cctactgttg gaccaattaa tgagccagta gttgatgcag ttgcaagagt acccaaagcc
     1501 catcctgaat gatttttatc tgtctgtgtt gcaataagag tagtacatgc agtagcataa
     1561 cccgtaaaaa ctccttgaag tattcttaat cctaataact gataaacatt ttgaacaaac
     1621 gccatggtaa atataactat tcccatccca atacttgcac gtaaaagcat tgattttctt
     1681 ccatatttat cagcagcttt ccctcaaatt ggcgaaaaga atgctgctac tacaaaggta
     1741 catccaaaaa ttatacctga atactgttgt attaaactaa catcagttac ccccaactgt
     1801 ttaatatata atggaagtat aggtgctatt tggctcatcc ctatagaact aaaaaacata
     1861 cctatccaac atacaaataa atttcttttc cacaattcca tatagaatta caccccctgc
     1921 attttaaatt ttttcaataa tactaagttt attaatttat gctattgttt actcaatata
     1981 tccatctttt cttttataat attgtttagg aaattcataa cttgaaaata atagcggtga
     2041 taaagaatag gagtaacttc cagcattact aattgaaatc aaatctccta tgtttgcttt
     2101 tttcaattta acattatttg caattacatc taatgatgta cagagattcc caacaacatt
     2161 tacatactct tcttctttta aattatttaa tactgaaaga gaaaaggcat gatttgatgt
     2221 atataatggc tcttgacctt ctaattctac tccacaagta atattttcaa ttaaattaga
     2281 aataataggt ctcataaatc cattaagtcc attttttaca ataagatatt ttttccctct
     2341 tgatacttta atatctacaa ttttaacata ataattacct gatttacaaa ctatgaaacg
     2401 tcctgattca ataagaagtc ttgattttag tgtgtttgaa ttagtaaaag caatcttttg
     2461 agttaatttt gaaagctctt taagattaag tggattttct ctaagattat cataagctac
     2521 tccaagtcca ctaccaaaat ttataaattc aatattaata tacttacttt tatttaaatt
     2581 taaagctata aaaaaacatt tttcataata atgtcctaac ttttcaaaat ccaatacctg
     2641 agatttaata tgtatatgaa tcccaactac agatacataa tcacacatac ttaacatctt
     2701 aaaaacactg ctcatatctt ctatatctat accaaatttg cttgatttag cactatcatc
     2761 atccatagta aaatcaggat ttattcttat tcctatttta actttctgat tatgcttttt
     2821 agatatctta tttataagtt ctatttctgt aatactatct gcaataatta tgcatttatc
     2881 taaaacagtt tctatatcaa tctctgtttt gcctggtgat gaataataaa tatcatcttt
     2941 agaaactcca cattttgatg aaatcataac ttcattagtt gaagaagcat ctgctccaaa
     3001 accttgctct gcaatgcttt ttacaatttt gttatatgga tttgttttta tagaatataa
     3061 aaaatcaaaa cctaaaatat tatttttcaa ttccttacat ttttcaatta tattagtctc
     3121 atcatatata taac
//
LOCUS       DAELLV010000315         3132 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.315, whole genome shotgun sequence.
ACCESSION   DAELLV010000315 DAELLV010000000
VERSION     DAELLV010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.315"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..593)
                     /locus_tag="KPD58_003472"
     CDS             complement(<1..593)
                     /locus_tag="KPD58_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0267197.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGAS
                     IVAFSLTNGISPVVATLMAILCGCTAGLVTGILHIKFKISNLLSGILVMGMLYSINLR
                     IMGKSNIPLFSFKHLFNGEISPIVLALAFVFICKVLLDLFLKTGLGYTLKGVGDNSQM
                     IKSLGINIGSIKILGLMISNGLIALSGSLMAQFLGFS"
     gene            complement(1402..2439)
                     /locus_tag="KPD58_003473"
     CDS             complement(1402..2439)
                     /locus_tag="KPD58_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0267198.1"
                     /translation="MIKSKKILSLIIAGVLGVSMLTGCSQNDGSNASNENKETDSKKQ
                     KNIGITQLVEHPSLDKAKKGFIKALEDKGYKDGDNIKIDFQNAQNDMPTTQSIASKFV
                     SDKKDLIYAISTPSAQAAYNATKDIPIIMTAVTDPVEAGLVKSLEKPGGNVSGTSDYL
                     SIDKTLELVKTLTPKAKKIGVIYNTSEVNSKIQVDSLHDYAKKNNYEVVEKGISSSSE
                     VNQAISSLVGKIDVLYVPTDNLIVSSMPIVSKVANENKIPIIASEEGSVSSGALACCG
                     IDYEKLGYKAGELAIEVLEGKSVGDIPVTTLDETEIIINEDTLKALDMQKLSADNIKY
                     IKSDENAKSAK"
     gene            complement(2536..>3132)
                     /locus_tag="KPD58_003474"
     CDS             complement(2536..>3132)
                     /locus_tag="KPD58_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003399531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0267199.1"
                     /translation="GLIKESSGQITLDSTTITNLAEYKRTQIVSRVFQDPSLGTCPSM
                     TVRENLSLALNKGKLLNLKKCLRHKTNDLEHLLEGISLDLKKYLDIKVQYLSGGQRQS
                     LSLIMSSLASPKVLLLDEHTAALDPKTSNEVIELTDKIVREKNITTLMVTHNLKHALQ
                     YGDRLIMLHKGEVVLDVKDKEKEDLTVEEILEKFEYAV"
BASE COUNT         1067 a          485 c          416 g         1164 t
ORIGIN      
        1 tctgagaatc ccaagaactg agccataaga ctaccagata aagctataag tccgtttgaa
       61 atcataagtc ctagtatttt tatagaaccg atatttatac ctagggattt aatcatttga
      121 gagttatcac caacaccttt taaagtgtat ccaagacctg tttttaggaa taaatctaaa
      181 aggactttac agatgaatac aaaagctaag gctaaaacta taggagatat ttccccattg
      241 aataagtgtt taaagctaaa tagaggaata tttgattttc ccattattct taaattgata
      301 gagtatagca ttcccataac taaaatacca gaaagtaagt tagatatctt aaacttaatg
      361 tgaagaatcc ctgtaacaag accagcagta caaccacata agatagccat taaagttgct
      421 actacaggag aaataccatt agtaagagaa aatgctacaa tagaagcacc cattgtgtag
      481 ctaccatcag cagacatatc tggaaaatct aagattttgt aagttatata aacccccagt
      541 gccataatcg ataaaattaa actttgcgtc attacagata taatacctga cataaataaa
      601 acactccttt gaaaatattt ttataatttt aattatttgc taattttgtg tataaaaaaa
      661 ccacatagtg tacaaaacta tgtggtaaaa acacgaataa gccacatagt ttaatctatg
      721 tggctcttat atagttaaat gaaaacttta taaacaatat agccaacata gatacaatcc
      781 caaaatttta gtagtcttaa ggggtctgta tctatgttaa aacgtaggta caaaccctaa
      841 aaatagtggc tataataata ataatatttt ctattgttta ataaagtatt cataattatc
      901 tctccctttt aatattttaa ttagtcagaa aattatatgt attctcgact tgttataaat
      961 tataaattat acaataataa aagtcaactg ctaaatttaa ttaaaatact aaataatgta
     1021 tactcagaat acttttaaat taatgtattg tttgcaaaaa acgttatcct aagttataag
     1081 tgaatgtgga aattttatca aaatatttac tagatatgga ataactagac atgtatatca
     1141 atcaaatatc ataaaaaaaa gctgtctgga gaatattaaa attcttttgg acagctcaag
     1201 tagtgattag aaagttatat aactttcata atcagtcgtc aatatagacg acttttatgg
     1261 aaaatgaata actttatatg caatattcaa attgctacaa taagcacaat tttcaaaggg
     1321 ttagggtgga taagagtact tattatataa agttttcata ttacctactt catattacct
     1381 attttttgaa attaataggt tttattttgc agattttgca ttttcatctg actttatata
     1441 ctttatatta tctgctgata acttttgcat gtcaagtgct tttagtgtat cttcgttaat
     1501 tattatttca gtttcatcta atgtagtaac tggtatatcg ccaacagatt ttccttctaa
     1561 tacttcaata gcaagttctc cagccttata acctagtttt tcataatcta ttccacaaca
     1621 agctaaagca ccagaagaca cagaaccttc ttcagaggct attataggaa tcttgttttc
     1681 attagcaact ttagaaacta ttggcataga agaaactatt aaattgtcag tagggacata
     1741 taaaacatct attttgccaa ctaaactaga aatagcttgg ttaacttcac ttgaagagct
     1801 gattcctttt tcaactactt cataattatt tttcttagca taatcatgta gagaatcaac
     1861 ttggattttt gaattaactt cactagtatt gtatataacc cctattttct ttgcttttgg
     1921 agtcaatgtt ttaaccaatt ctaatgtttt atcaattgaa agataatcag atgtaccaga
     1981 aacatttcca cctggttttt caagagattt aacaagtcca gcttctacag ggtctgtaac
     2041 agcagtcatt attatcggaa tatctttagt agcattataa gctgcttgtg cagatggagt
     2101 agatatagca tatatcaaat cttttttgtc ggatacaaac ttactagcaa tactttgtgt
     2161 agtaggcata tcattttgtg cattttggaa atctattttt atattatctc catctttata
     2221 gcctttatct tcgagtgctt tgatgaatcc tttctttgct ttatccagag atggatgttc
     2281 cactaattga gtaataccta tatttttttg ttttttgcta tctgtttctt tattttcatt
     2341 tgaagcattg gagccatcat tttgagaaca tccagtaagc attgatacac caagtactcc
     2401 tgctataatt aaacttaata tttttttact ttttatcatt ttattttcct cccttaaaat
     2461 ttattaatta tatttgttca tatattcata tgatagatta taaatatttc tgattaaaac
     2521 taagatttta atttcttata ctgcatattc aaatttttct aagatttctt caactgttaa
     2581 atcttctttt tctttatctt ttacatctaa tacaacttca cccttatgaa gcattatcaa
     2641 cctatctcca tattgaagag cgtgtttcaa gttgtgagta accataagtg tagttatatt
     2701 tttctctctt acgattttat ctgttaactc tattacttca tttgatgttt ttgggtcaag
     2761 tgctgctgta tgttcgtcta gtaataagac cttaggactt gctaagctag acataattag
     2821 tgatagtgat tgtctttgac cccctgaaag atattgtact ttaatatcta aatatttttt
     2881 taaatccaaa gaaattcctt caagtaagtg ttccaagtca ttcgttttat gacgaagaca
     2941 tttttttaag ttcaataatt tccctttatt aagtgctaga gataaatttt ctctaaccgt
     3001 catagaagga caagttccaa ggcttgggtc ttgaaaaact ctacttacta tttgagttct
     3061 tttatactca gctaaatttg ttatagtagt actatctaaa gtaatttgac cagaagattc
     3121 ttttataagc cc
//
LOCUS       DAELLV010000316         3070 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.316, whole genome shotgun sequence.
ACCESSION   DAELLV010000316 DAELLV010000000
VERSION     DAELLV010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.316"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1096
                     /locus_tag="KPD58_003475"
     CDS             <1..1096
                     /locus_tag="KPD58_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HBG0267200.1"
                     /translation="ISDESMDVIDLYDDNNNEVAKKVNFSIFNPKFNIYMYQYKKLIN
                     YLDKSICMGALSSGSTSVPKVLYRTYESWAGFFPIQNGIFNISGESILFVNGTLSFTG
                     NLNSIMSVLYEGGSIVISSGLNCKSWIRTIKQYNVTNIYLIPTKLQLLVKHLKEPVLK
                     VVSIFTGSQLLFEDTARNLKKYMPKSEIILYYGASELNYITYLCYDELIEKPLSVGRP
                     FPGIDIYIREGKIFVNTEYAVYNATKPYSVNDIGYYDNDGYLIFEGRSDDIINIGGFK
                     VSSTKIENEVKKIPQIEDAIVLPYSDTIRGNQIVLFVITIDKITKKDLLIKMKDNLMK
                     NEIPKKIIFLNSFPYTSSEKIDRLALLKML"
     gene            1172..2047
                     /gene="dpsA"
                     /locus_tag="KPD58_003476"
     CDS             1172..2047
                     /gene="dpsA"
                     /locus_tag="KPD58_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HBG0267201.1"
                     /translation="MSNQYDITVIGGDLRQVYMVKKLINKGYSVVTYGISNEIINGIV
                     GKSTSLAEALSKSSIILCPIPFTKNQVNIENTDDCLDATIDNLLINLSPNQILFGGNI
                     PTKVCKFCEQHSIQIYDFMKMNDVAILNAVATAEGAIAEAIKRSIINIHQSNCLVLGY
                     GRCAQILAKKLYALDANVCVGARNNDARSTANAFGYSSISLSQLDEKLLQFDYIFNTI
                     PECILTKERLEKVSQEVTIIDIASSPGGVDYSVVKEAGINATLCLGLPGKYSPKTSGE
                     ILVNAIENIINERRD"
     gene            2050..2640
                     /locus_tag="KPD58_003477"
     CDS             2050..2640
                     /locus_tag="KPD58_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HBG0267202.1"
                     /translation="MRLEGLTIGVGFTGSFCTYDKIFIELENLVKEGANVHTIFSDVS
                     QNIDCRFGNSEEFMKKAYELTGNKPIVTIEEAEPFGPKGIADIIIIAPCTGNTAAKLA
                     NGITDSPVLMAAKGHLRNDKPLVISISTNDALSFNFKNIGILLNSKNIYFVPFGQDNC
                     KAKPNSMIAHTELIIPTIELALENKQLQPVIKSPHQ"
     gene            complement(2847..3047)
                     /locus_tag="KPD58_003478"
     CDS             complement(2847..3047)
                     /locus_tag="KPD58_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0267203.1"
                     /translation="MEKLKTISVFSLIISVILTIGGIGIVTYYVDNLFIRGLSVFVLI
                     MSSSFVSTTVRLIFEESKRYKF"
BASE COUNT         1172 a          387 c          481 g         1030 t
ORIGIN      
        1 tatatctgat gaatctatgg acgttattga tttatatgat gataacaata atgaagtagc
       61 aaaaaaagtt aatttttcta tttttaatcc taaatttaat atatatatgt atcaatataa
      121 gaagcttata aattatttag ataaaagtat ttgtatggga gctttatctt caggttctac
      181 aagtgttcca aaggtattat atagaacata tgagagttgg gctggatttt ttcctataca
      241 aaatggtatt tttaatatct ctggggaatc aatcttgttt gttaatggaa cacttagttt
      301 tacagggaat ctaaattcta taatgtcagt attatatgaa ggaggtagta ttgtaataag
      361 ttcaggtctt aattgtaaat catggataag gacaataaaa caatataatg taactaacat
      421 ataccttata cctacaaaac ttcaactttt ggttaaacat ttaaaagaac cagtactcaa
      481 agttgtaagt atttttacag ggtctcaatt attatttgaa gatactgcaa gaaatctaaa
      541 aaaatatatg ccaaagtctg aaattatttt atactatggg gcaagtgaat taaattatat
      601 tacatattta tgctatgatg aactaattga aaaacctcta agtgtaggca gaccatttcc
      661 aggaatagat atatatatta gagaaggaaa aatttttgtg aatacagaat atgcagtata
      721 taatgcaacc aaaccttatt ctgtcaatga tattggatat tatgataatg atggatactt
      781 aatatttgaa ggaagaagtg atgacattat aaatattgga ggatttaaag ttagttctac
      841 taagattgaa aatgaagtta aaaaaattcc tcaaattgaa gatgcaattg tattgccgta
      901 ctcagacact ataagaggaa atcaaattgt attatttgta ataactatag acaaaattac
      961 gaagaaagat ttgttgatta aaatgaaaga taatctaatg aaaaatgaaa tcccaaagaa
     1021 aattatcttt ttaaattcat ttccatatac ctcttctgaa aagatagata gattagcatt
     1081 attaaaaatg ttataagtat aatatttagt aattttagtg aaccaatgta ttgataaaaa
     1141 catattgtat atagtaaagg aggcttacaa tatgtcaaat caatacgata taactgttat
     1201 tgggggagac ctgcgacaag tgtacatggt aaaaaaacta attaataaag ggtattctgt
     1261 agtcacttat gggatatcaa atgaaattat aaatggaatc gttggaaaat caacttcact
     1321 agctgaagca ctatccaaaa gttccattat tttatgtcca attccattta caaagaatca
     1381 agttaatatt gaaaatacgg atgattgtct tgatgctact attgataact tgctaattaa
     1441 tttatctcct aatcaaatat tatttggagg taacatacca actaaagttt gtaaattttg
     1501 cgagcaacac tctattcaga tttatgattt tatgaaaatg aatgatgttg ctattttaaa
     1561 tgcagttgct acagctgaag gggctatagc agaagctatt aaaagaagta taatcaatat
     1621 acatcaaagt aactgtttag tattaggtta tggaagatgt gcacaaatac ttgcaaaaaa
     1681 attatacgct ttggatgcaa atgtatgtgt tggcgcaaga aataatgatg caagaagtac
     1741 ggctaatgca tttggatatt ccagtatttc tttaagtcaa cttgatgaaa aacttttaca
     1801 atttgattat atttttaaca ccataccaga gtgtatttta acgaaagaaa ggttagaaaa
     1861 agtatcacaa gaagtaacta ttatagatat agcttcttct cctggaggag tagattattc
     1921 tgttgttaaa gaagcaggga ttaatgcgac tctttgcctt gggcttcctg gtaaatattc
     1981 accaaagaca tctggtgaaa ttcttgttaa cgctatagaa aatattataa atgaaagaag
     2041 ggattagaaa tgagacttga aggacttaca attggagtag gatttactgg ttcgttttgt
     2101 acttatgata aaatatttat agagttagag aatttggtta aagaaggagc taatgtacac
     2161 actatatttt cagatgtatc acaaaatatc gattgtagat ttggaaattc tgaagagttt
     2221 atgaaaaaag catatgaact tacaggaaat aaacctatag ttacgattga agaggcagag
     2281 ccatttggac ctaaaggaat tgctgatatt ataataatag ctccttgtac tggaaatact
     2341 gctgcaaaat tagcaaatgg tattacagat tcaccagtat taatggcagc aaaaggtcat
     2401 cttcgtaatg ataaaccact agtaatatca atatcaacga atgatgctct aagctttaat
     2461 ttcaaaaata ttggcattct ccttaattct aaaaatatat attttgtacc ttttggtcaa
     2521 gataattgca aagcaaagcc taattctatg attgcacata ctgagctaat aattccaact
     2581 atagaacttg ctctagagaa caaacagcta cagcctgtta taaaaagtcc acatcaatag
     2641 aattggttct tataatatta tgtattagat acaatactag caaagaggct gtctcaaaat
     2701 agagagaagc ctctttttct tgttgaaatg atgaaaatat atttaatttc atacaaaaaa
     2761 atgcctcatg gactaaaaag tctattttga gctaatctcc gtaatttttg ctatattaaa
     2821 attatttact taatccaatt tttaaattag aatttatatc gttttgactc ttcaaatatc
     2881 aatctaactg ttgttgatac aaaagaagag gacataatta aaacaaatac tgatagtcct
     2941 ctaataaaca aattatctac ataataagtt actattccaa tacctccaat cgttaaaatt
     3001 acacttataa tcaaagaaaa aacgctaatt gtttttaatt tttccatgct tatcctcctt
     3061 cttataaact
//
LOCUS       DAELLV010000317         3060 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.317, whole genome shotgun sequence.
ACCESSION   DAELLV010000317 DAELLV010000000
VERSION     DAELLV010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.317"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            238..930
                     /locus_tag="KPD58_003479"
     CDS             238..930
                     /locus_tag="KPD58_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0267204.1"
                     /translation="MSYKVYIVEDDISISTLLKDYIEKYGFEVIVEENFDDIMITFES
                     FKPHLVLLDVNLPKFDGFYWCRCIRQQSNLPIIFISARDGNVDQIRALESGADDYITK
                     PFYYDVVMAKIKSHIRRVYGEYSPKIDERIVELDGLKFYPERLELELNGENLIITKKE
                     GILIECLIKRYPKVVSRDYLLEKIWDDIEFVEENTLNVNVSRIRKRFEELGIDNAVQT
                     VRGLGYKLNKNW"
     gene            940..1944
                     /locus_tag="KPD58_003480"
     CDS             940..1944
                     /locus_tag="KPD58_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0267205.1"
                     /translation="MKLFLKDYKGYIFIYYIGIVMTIIYCNLMKFISFGESLYILLFS
                     SFILLCFLIYKYYKNREVYNLFEKGVHSLEESFLYLGSSFLGRNISVILKQQHKLYQS
                     MIQEHKKIHSEHLTFINQWIHQMKTPISVISLQLQDYEGEEVTENIRKEIDRLNKGLD
                     MAMHFARADNFQKDFIVEKAKLNEIVSSIVNEEKRLFIKSGMIPKIQVDKFTYVYTDV
                     KWMKFIIGQLITNGVKYSKDYSNYLTIKSEENNKYVILSIIDEGIGIVSKDLKRVFDP
                     FFTGENGRKFGESTGMGLYLVKKVCDDLGHKVSIKSEVGKGTEVSIYFNKDVNSLIKV
                     "
     gene            2192..3043
                     /locus_tag="KPD58_003481"
     CDS             2192..3043
                     /locus_tag="KPD58_003481"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBG0267206.1"
                     /translation="MSLDVIFILKSVIIAIVEGLTEFIPVSSTGHMILVGNLIDFKGQ
                     FAEMFEVVIQLGAILAVVVLYWKKIKDSVIEFFKFIFTGGKEGKIGFRFGMNVIIGCI
                     PFAIIGVLFYDNIKSLFNLQSVIIGFIVGGILLLVVETLFRKKNHSTDNIDKITPIQA
                     LKVGTLQVLSAWPGMSRSASTIMGGWIAGLNSPTAAEFSFFLAVPAMVASSGKDLFEF
                     DYSIMTPTLWIALVVGFIVAFIVSIIVMEKFVNFLKKKPMRVFAVYRIIMGVVLAVLA
                     FTNIISV"
BASE COUNT         1171 a          243 c          565 g         1081 t
ORIGIN      
        1 ttgaattagt aatttcaatt tattttagaa tgattagttg ataataatga ttaattaatc
       61 ataaaaagat acttttttat agagttttat taagatgtct aataatatgg ttatacttat
      121 gaagtcagta attgtgaagt tagtaatatg attaaattta tatattgcaa aaagattata
      181 atatattaaa attggactgt tatatgatga gtaaataggt atattaggag gaattatatg
      241 agttataaag tatatattgt ggaagatgat atatcaataa gtacattact taaagattat
      301 attgagaaat atggatttga agtgattgtt gaagagaact ttgatgatat aatgattaca
      361 tttgaaagtt ttaagccaca cttagttcta ttagatgtaa atttacctaa atttgatggt
      421 ttctactggt gtagatgtat taggcaacag tcgaatttac ctataatttt tatatcagct
      481 agagatggta atgtagacca aattagagct ttagaaagtg gagcagatga ttatataaca
      541 aaaccttttt actatgatgt agtaatggca aaaataaaga gtcatataag aagggtatat
      601 ggtgaatatt cacctaaaat agacgaaaga atagtggagc tagatggatt aaagttttat
      661 ccagaaaggt tagaactaga attaaatggt gagaatctaa ttataactaa aaaagaaggt
      721 atactgattg aatgtcttat caagagatac ccaaaagtag taagtagaga ttatctttta
      781 gaaaagatat gggatgatat tgagtttgta gaggaaaata ctttaaatgt aaatgttagc
      841 aggataagga aaagatttga ggaattaggt atagataatg ctgttcagac tgtccgaggg
      901 ttaggatata aattaaataa gaattggtaa ggagaaagta tgaagttatt tttaaaagat
      961 tataaaggtt atatttttat atattatata ggcatagtta tgacaattat ttactgtaat
     1021 cttatgaagt ttattagttt tggagagagt ttatatattc ttttatttag ttcttttata
     1081 ttgttatgtt ttttaatata taagtattat aaaaatagag aagtatataa tctgtttgaa
     1141 aaaggagtac attctttgga ggagtcattt ttgtatttag gaagctcatt cttggggaga
     1201 aatatatcag ttattttaaa acaacaacat aaattgtatc aatcaatgat acaagagcat
     1261 aaaaagatac atagtgaaca tttaactttc atcaaccaat ggattcatca gatgaaaaca
     1321 ccaatatcag ttataagcct tcagcttcaa gattatgagg gagaagaagt aactgaaaat
     1381 ataagaaaag aaatagatag attaaataaa gggttagata tggctatgca ttttgcaagg
     1441 gcagataatt ttcagaaaga ctttatagtt gaaaaagcca aattaaatga aattgtatct
     1501 agtattgtaa atgaagaaaa aaggttattt ataaaaagtg gaatgattcc taagattcaa
     1561 gtagacaaat ttacttatgt ttatacagat gtaaaatgga tgaaatttat aataggtcaa
     1621 ttaattacaa atggagtgaa atattctaaa gattatagta actacctaac tataaaatct
     1681 gaagaaaata ataaatatgt aattttaagt ataatagatg aaggaattgg aattgtttct
     1741 aaagatttaa aaagggtatt tgacccattt tttacaggag aaaatggtcg taagtttgga
     1801 gaatctacag gtatgggtct ttatctagta aaaaaggtat gtgatgattt aggtcataaa
     1861 gtaagtataa aatcagaagt aggaaaaggt acagaagttt caatctactt taataaagat
     1921 gtaaatagtt taataaaggt gtaaattatc tgttacaata gggtttttag ttaattgtac
     1981 ttttttatta tacaatttat taaatgaaat attcatagtt aaataatcaa aatttaagag
     2041 agtttatttg caaaccttac atagatgtaa ggtttgtgta atgtttgcag atagttaaat
     2101 tttctaatct aaattacaat taatataaga aataacataa gagaaataac ataaaaaaag
     2161 aaattttgta aaaaaggaag gatgtttatt gatgagttta gatgttattt ttatattgaa
     2221 atcagtcata atagctatag tagaggggct tacagaattt attccagttt catctacagg
     2281 tcatatgatt ttggttggta atttaataga ttttaagggg caatttgctg aaatgtttga
     2341 ggttgtaata caacttgggg ctatattagc agtagtagta ctttattgga aaaagataaa
     2401 agatagcgtt atagaatttt ttaaatttat atttactggc ggaaaagaag gaaagattgg
     2461 ttttagattt ggaatgaatg taattatagg ttgtatacca tttgcgatta taggagttct
     2521 attttacgat aatataaaat cattgtttaa tttacaatct gtaattatag gatttatagt
     2581 gggtgggatt ttattattag tagtagaaac tttgtttaga aaaaagaatc attccacaga
     2641 taatatagat aaaattactc caatacaagc tttaaaggtt gggacattac aagttttgtc
     2701 tgcatggcca ggtatgtcaa ggagtgcatc tactattatg ggtggatgga tagcaggact
     2761 taattctcct acagcagctg agttttcttt ctttttagca gtaccagcta tggtggcttc
     2821 ttcaggaaaa gatttatttg aatttgatta ttcaataatg acaccaacat tgtggattgc
     2881 tttggtagtt gggtttatag tagcttttat tgtatcaata atagttatgg aaaaattcgt
     2941 gaatttttta aagaaaaaac caatgagagt gtttgctgta tacagaataa tcatgggtgt
     3001 agtacttgca gtattagctt ttactaacat aattagtgta tagaaaaaat agatttatta
//
LOCUS       DAELLV010000318         3055 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.318, whole genome shotgun sequence.
ACCESSION   DAELLV010000318 DAELLV010000000
VERSION     DAELLV010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.318"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(201..2588)
                     /gene="cadA"
                     /locus_tag="KPD58_003482"
     CDS             complement(201..2588)
                     /gene="cadA"
                     /locus_tag="KPD58_003482"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBG0267207.1"
                     /translation="MEANNSIKKEFILGGLNCAHCAEEINNKVSKLQEVKSSNLNFIN
                     KKLTVNIKESFNEDTTIEKIIDIIDSTEPGLDIQISSKENAASKTSIKKELILGGLNC
                     AHCAEEINNKVSKLKEVESSNLNFVNKKLTVNISNNFEEDDVINKIKEIINSTEPGLD
                     IQVGSTDKVKGRTTEKSGAVNDTNKKELIPLIIGALVYIFGIYQTATGYESQFSNIVF
                     IVAYVIVGGDVLLRAIRNISKGRVFDENFLMALATVGALTIGELSEAVGVMLFYKVGE
                     YLQGVAVGKSRKSITSLMQIRPDYANLKVNSEVKVVSPEEVNVGDIIVVKPGEKVPLD
                     GVVVDGVSMLDTSALTGESVLREVEKGDEILSGVINKNALLSIEVTKSFGESTVSKIL
                     DLVENSSIKKSKTENFISKFSRYYTPIVVIAALLIAFVPPLVISGEVFSDWLYRGLIF
                     LVVSCPCALVLSIPLSFFSGIGFASKNGILIKGSNYLEALRSVDIVVFDKTGTLTKGV
                     FNVTKLNPEGISEEELLEYAAIAEVNSNHPIAKSILSYYNKKIDLDTIDSYEEIAAYG
                     IRVKHNGNFILAGNEKLMKKENISYSSAKEVGTVVYIAVDKVYRGYIVISDEVKEDSK
                     NAIRSLKEIGVKEVVMLTGDNEKVAKNIAQELELDTVYSNLLPNEKVDRLEDLYEGRT
                     EKEKIAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVLMTDEPSKISKAIEIA
                     NKTNKIVWQNIIFALGVKIIVMILGAGGVATMWEAIFADVGVALIAVVNAMRAMR"
     gene            complement(2611..2976)
                     /locus_tag="KPD58_003483"
     CDS             complement(2611..2976)
                     /locus_tag="KPD58_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0267208.1"
                     /translation="MREEINDCNCNIVHEEIVTEAKSTMPDEEMLYDLAELFKVFGDT
                     TRVKILYALFANEMCVCDIASLLNMTHSAISHQLRVLKQARLVKFRREGKTVYYSLDD
                     SHISQIFDCGLNHIRETYK"
BASE COUNT          958 a          597 c          303 g         1197 t
ORIGIN      
        1 aaaattacaa tataaaagct ctacactaaa taaatacatt aaataaacat aatggataat
       61 gaatataaat ttatatatgc aaagcgctcc aataaaaagg taccaataaa gttaaaagaa
      121 acttattgat acctttcatt attacatact tcaatatata attataagtt ttttaaatag
      181 tatggattta catttatccc ttatctcata gctctcatag catttactac tgctataagt
      241 gccacaccta catcagcaaa tatagcttcc cacatagtag ctactcctcc agcacctagt
      301 atcataacta taatttttac tcctagagca aatattatgt tttgccacac tatcttatta
      361 gtcttgtttg ctatttcaat tgctttagat atcttgctag gctcatctgt cataagcact
      421 acatctgcag cttcaatagc agcatctgaa cctaagcctc ccatagctat accaacatca
      481 gcacgagcta atactggagc atcatttatt ccatcaccta caaacgctat tttctctttt
      541 tcagttcttc cttcatataa atcttctaat ctatctacct tttcattagg aagtaaattt
      601 gagtaaactg tatccaattc taattcttga gcaatatttt ttgctacttt ctcattatct
      661 ccagttaaca ttacaacttc ttttacccct atttctttta agcttcttat ggcattttta
      721 ctatcttctt ttacttcatc agagataact atataacctc tatatacttt atcaacagca
      781 atatagacta ctgtgcctac ttctttagct gatgaataag atatattttc ttttttcata
      841 agtttctcat tacctgctaa tataaaattg ccattatgct ttactctaat tccataagct
      901 gctatttcct cataactgtc tattgtatct aaatcaattt ttttattata gtaacttaat
      961 atggactttg ctattggatg atttgaattt acctccgcaa tagctgcata ttctaataat
     1021 tcttcttctg atataccttc aggatttaac ttagttacat tgaacacacc tttagtaagt
     1081 gttcctgttt tatcaaatac tactatatct acacttctta aagcttctaa atagttactt
     1141 cctttaataa ggataccatt tttagatgca aacccaattc cactaaaaaa actaagtggt
     1201 attgataaaa ctaatgcaca agggcaagaa acgactaaga atatcaatcc tctatataac
     1261 caatcactaa atacctcacc tgagattaca agtggtggca caaatgctat taataaagca
     1321 gctattacta ctattggagt atagtatctt gaaaacttag atataaagtt ttctgtcttt
     1381 gattttttaa tgctagaatt ttcaacaagg tcaagaatct tagaaactgt tgattctcca
     1441 aaactctttg taacttcaat actcaataaa gcatttttat ttataacacc tgataagatt
     1501 tcatctcctt tttcaacttc tctcagcact gactctcctg tgagtgcaga agtatctaac
     1561 atagaaactc catctactac tacaccatct aatggtactt tttcaccagg ttttactact
     1621 attatatcac ctacatttac ctcttctggt gacaccacct taacttcaga attaactttt
     1681 agatttgcat agtctggtct tatttgcata agtgaagtta tagactttct ggatttacca
     1741 actgcaacac cttgtaaata ctctcctact ttatagaaaa gcataacccc tactgcttct
     1801 gataactccc ctattgttaa tgctcctact gttgctagag ccatcaaaaa gttctcatca
     1861 aatactcttc cttttgatat atttcttatt gctcttaaaa gcacatcacc acctactatt
     1921 acataggcaa ctataaatac tatattgcta aactgacttt cataacctgt agctgtttga
     1981 tatataccaa atatatatac aagtgctcca attataagtg gaattaattc tttcttattt
     2041 gtatcattta cagctcctga tttttcagta gttctccctt ttactttgtc agtagaccct
     2101 acttgtatat caagccctgg ctctgttgaa tttattattt cttttatttt atttataaca
     2161 tcatcctctt caaagttatt acttatattt actgtaagtt ttttatttac aaagtttaaa
     2221 ttagaagatt caacttcttt taatttagaa actttgttat taatctcttc tgcacaatga
     2281 gcacagttta atcctcctaa tataagctct tttttaattg atgttttact agctgcattt
     2341 tctttagaac ttatttgtat atcaagacct ggctctgttg aatctattat atctattatc
     2401 ttttctatag tagtatcctc attaaaactt tcctttatat ttactgtaag ttttttattt
     2461 ataaaattta aattggaaga tttaacttct tgtagtttag aaactttatt attaatctcc
     2521 tctgcacagt gagcacaatt taatcctcct agtataaatt cttttttaat tgaattattt
     2581 gcttccatat atattcactc ctctcattta tcatttataa gtctctctaa tatgatttaa
     2641 tccacaatca aatatctgac ttatatgact atcatctaat gagtaataca ctgtttttcc
     2701 ttctcttcta aactttacta atctagcctg ctttaatact ctaagttgat gagatattgc
     2761 agaatgagtc atatttaata aactcgctat atcacataca cacatttcat ttgcaaataa
     2821 tgcatataat atctttactc tagtagtatc cccaaatact ttaaataatt ctgctaaatc
     2881 atataacatt tcttcatctg gcatagttga tttagcctct gttactattt cttcatgtac
     2941 tatattacaa ttacaatcat ttatctcttc tctcattttt aatcctccat tattttaata
     3001 tatgaataac tgttcatatg ttcattatat gttatcattt ctaaatttgc aacaa
//
LOCUS       DAELLV010000319         3034 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.319, whole genome shotgun sequence.
ACCESSION   DAELLV010000319 DAELLV010000000
VERSION     DAELLV010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.319"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            246..2888
                     /gene="adhE"
                     /locus_tag="KPD58_003484"
                     /gene_synonym="adhC"
     CDS             246..2888
                     /gene="adhE"
                     /locus_tag="KPD58_003484"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBG0267209.1"
                     /translation="MEKKEKVVEKSNVEVCSPEIVNSVETLRMRLEEIRLAQKEFATF
                     TQEQVDKIFLAASTAANQQRIPLAKMAVEETGMGIVEDKVIKNHFASEYIYNAYKDTK
                     TCGVIEKDEAFGFTRIAEPVGVLSAVIPTTNPTSTAIFKSLIALKTRNGIIFSPHPRA
                     KNCTIEAARVVHDAAVKAGAPKGLIGWVDVPSIELTNVVMAEADLILATGGPGMVKSA
                     YSSGKPAVGVGPGNVPAIIDESADIKMAVSSILVSKSFDNGMICASEQAVIVPEKIYE
                     EVKKEFKYRGAHFLNKEETEKVGKVVIIDGSLNARIVGQPAHVIAKMADVEVPKTARI
                     IIGEVESVELNEPFAHEKLSPVLAMYKSKSFEDAVAKAEKLVADGGYGHTSSLYADSI
                     NHPERVEKFVNAMKTCRVLVNTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVSENVG
                     VKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVAAREFKDVMDKKKAFIVTDSFLY
                     NNGYTKKLTDLLDEMGIKHTTFFDVAPDPTLACAREGAKAMADFQPDLIIAVGGGSAM
                     DAGKIMWVMYEHPEVDFQDLAMRFMDIRKRVYVFPKMGEKAYFAAIPTSAGTGSEVTP
                     FAVITDQDSGVKYPLADYELMPNMAIIDADMMMEMPPRLTAASGVDALTHALEAYVSM
                     LRTEPADGLALQAGKIIFEYLPRAYKNGKNDKEAREKMAMASTMAGMSFANAFLGICH
                     SLAHKLGAFHHVQHGVANALLINEVIKFNCAEAPNKMGAFSQYRYPDCIQRYAEFASF
                     AGIKGSTDQEKVDNLIKAIDELKAKVGLPKTIKEAGVEESKFLERLDAMVEQAFDDQC
                     TGANPRYPLMSELKEIYLKVYYGK"
BASE COUNT         1157 a          394 c          603 g          880 t
ORIGIN      
        1 atgaaatata tctcatttag ataaaaaatt ttagtaaaag taaaaaaaat aatatatttg
       61 ttaaaaaata aattgacaaa atattcagaa aaaaacttct tgacctaaga gagtaaaata
      121 aataaaatct aaggtataga gatgaaatct ataaaattat tttaagtata agttaaaaat
      181 atagatgatg catttaagat ttataaaaat tagagatgca taattatttt agggaggttt
      241 ttagtatgga gaaaaaagaa aaggtagtag aaaaatcaaa tgttgaagta tgtagtcctg
      301 aaattgtcaa cagtgttgaa acattaagaa tgagattaga agaaattaga ttggcacaaa
      361 aagaatttgc aacatttacg caagaacagg tagataagat tttcttagct gcttcaactg
      421 cagctaatca acaaagaata cctctagcaa aaatggcagt ggaagagact ggtatgggta
      481 ttgttgaaga taaggttata aaaaaccatt ttgcctcaga atatatctat aatgcttata
      541 aagatactaa aacttgtggt gtcatagaaa aagatgaagc atttggtttt acaagaattg
      601 ctgagccagt aggtgtttta tcagcagtta taccaacaac taatcctaca tcaactgcaa
      661 tattcaaatc cctaatagca ctaaaaacta gaaatggtat aattttctct cctcatccaa
      721 gagctaaaaa ctgtactata gaagcagcta gagttgtaca tgatgcagca gttaaggcag
      781 gagcaccaaa aggacttata ggttgggtag atgttccttc gattgaatta actaatgttg
      841 tcatggcaga ggcagacctt atattagcta ctggtggacc tggaatggtt aaatctgcat
      901 actcttcagg taaaccagct gtcggagttg ggcctggaaa tgtacctgca ataatagatg
      961 agagtgcaga tataaaaatg gctgtaagtt caatcttagt atcaaaatca ttcgataatg
     1021 gtatgatatg tgcttccgag caagctgtaa tagtaccaga aaaaatatat gaagaagtta
     1081 aaaaagaatt taaatatcgt ggagcacatt tcctaaataa agaagaaact gaaaaagtag
     1141 gaaaagtagt aataattgat ggttcattaa atgccagaat agttggacaa ccagcacatg
     1201 taatagccaa aatggcggat gtagaagttc caaaaacagc tagaataata attggtgaag
     1261 tagaatctgt tgaattaaat gaaccttttg cacatgaaaa actttctcct gtacttgcaa
     1321 tgtacaagtc taaaagtttt gaagatgcag ttgctaaagc agaaaaatta gttgcagatg
     1381 gaggctatgg tcatacatct tcattatatg cagactctat aaatcatcca gagagagtag
     1441 aaaagtttgt aaatgctatg aagacttgta gagtattagt taatactcca tcatcacaag
     1501 gtggtatagg tgatttatat aactttaaat tggcaccgtc tcttactctt ggttgtggtt
     1561 cttggggtgg aaactcagta tctgaaaacg ttggagtaaa acatttgctt aatatcaaga
     1621 cagtagctga aaggagagag aatatgcttt ggttcagagc accagaaaag gtttatttca
     1681 aaaaaggatg tttaggtgta gcagcaagag aatttaagga tgttatggat aaaaagaaag
     1741 catttatagt aacagattct ttcctatata ataatggata tactaagaaa ttaactgatt
     1801 tattagatga gatgggaatt aagcatacta cattctttga tgtagctcca gacccaacat
     1861 tagcttgtgc aagagaagga gctaaagcta tggctgattt ccaaccagat ttaataatag
     1921 ctgtaggtgg aggaagtgca atggatgctg gtaagataat gtgggtaatg tatgagcatc
     1981 cagaagtaga tttccaagac ttagctatga gatttatgga tataagaaaa agagtatatg
     2041 tatttcctaa gatgggtgaa aaagcgtatt ttgctgctat cccaacttca gcaggaactg
     2101 gttcagaagt aactccattt gcagttataa ctgaccaaga ttcaggtgta aagtacccat
     2161 tagcagatta tgaattaatg ccaaacatgg ctataattga tgctgatatg atgatggaga
     2221 tgccaccaag attaacagca gcatcaggtg ttgatgcttt aacacatgca ttagaagcat
     2281 atgtatctat gttaagaact gaaccagctg acggtctagc actacaagca ggtaaaataa
     2341 tatttgaata tttacctcgt gcatataaaa atggaaagaa tgataaagaa gcaagagaaa
     2401 aaatggcaat ggcatctact atggcaggaa tgtcttttgc aaatgccttc ttaggaatat
     2461 gtcactcttt agcacataaa ttaggtgcat tccaccatgt tcaacacggt gttgcaaatg
     2521 cattattaat aaatgaagta atcaagttca attgtgcaga agctccaaat aagatgggtg
     2581 cattctcaca atacagatat cctgattgca tacaaagata tgctgaattt gcttcttttg
     2641 caggtattaa aggaagtaca gaccaagaaa aagtagataa tcttataaaa gcaatagatg
     2701 aactaaaggc aaaagtaggt cttcctaaga ctataaaaga ggctggagta gaagaatcta
     2761 aattcttaga aagattggat gctatggttg aacaagcttt tgatgaccaa tgtacaggtg
     2821 ctaatcctag atacccattg atgagtgaat taaaagagat atatctaaaa gtttattatg
     2881 gtaaataata tctattgtgc aacataggtt ctattgggtt tgtatataaa ttattagatt
     2941 tgaatataaa tagagtttta ttagagacac gtagaagtaa ctataaataa tagattataa
     3001 tttaaattct aaatggaaat gataaaaagt cagt
//
LOCUS       DAELLV010000320         3030 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.320, whole genome shotgun sequence.
ACCESSION   DAELLV010000320 DAELLV010000000
VERSION     DAELLV010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.320"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            132..797
                     /locus_tag="KPD58_003485"
     CDS             132..797
                     /locus_tag="KPD58_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBG0267210.1"
                     /translation="MIKLIGILIVVIGFILKIDTLFTVLLAGVATGIVAGLDFNQIFT
                     ILGDSFVSNRGVSLFILTLPVIGVLERYGLKQRAVSLIEKLKRLTTGKVLTIYMIARQ
                     IAGALSIRMSGHPQFVRPLVNPMAQAAGLSNSDELKESDEEAIKALSAASENYGNFYG
                     QNLFAGSSGVLLIASTLTQFGYNVTGLNIVKASTIMAVIALVVATIQFTLYDKKLNKS
                     HKK"
     gene            819..1751
                     /locus_tag="KPD58_003486"
     CDS             819..1751
                     /locus_tag="KPD58_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBG0267211.1"
                     /translation="MQNFINISLEIFYALMGFLMIVIAYKSFTTINNNKKYGTSLFWI
                     LISLPFIFGRLIPANIIGIILILSSLLTLSKQVVFAKYEEPDENFGKEQADKLKNKIF
                     IPSLILAFAAVVVAMSLSNFDNSSQFAIGVGSIIALISALIITKAKPATSVQDGSRLL
                     QQMGPASMLPQLLVALGALFTQAGVGEVISTMISGVVPADSRLFGVIAYVLGMVIFTM
                     IMGNAFAAFSVITAGIGIPFVLSQGGNPAIIGALALTAGYCGTLLTPMAANFNIVPAA
                     LLECKNDYIVIKYQAPVALVLIIAHILVMYFLGF"
     gene            1772..2413
                     /gene="pcp"
                     /locus_tag="KPD58_003487"
     CDS             1772..2413
                     /gene="pcp"
                     /locus_tag="KPD58_003487"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBG0267212.1"
                     /translation="MKILLTGFDPFGGEPINPAQEAVERVNNNINGAEIIKITIPTVM
                     TKSVEAIDKAIQEHNPDIVISVGQAGGRFDITPERVAINIDDFRIKDNEGNQVIDTII
                     KEDGEPAYFSKLPVKAMVKHMNENKIPASVSNTAGTFVCNHVMYGILYMIDKKYPNIR
                     GGFIHIPYTTSQVIDKKNTPFMSLEEIVKGLELAIEACIIYKEDVKEIGGEIS"
     gene            complement(2533..2865)
                     /locus_tag="KPD58_003488"
     CDS             complement(2533..2865)
                     /locus_tag="KPD58_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0267213.1"
                     /translation="MSIDCISNTDLSETGFSYTLSLISGKYKMIILYCLVEFEVVRYN
                     ALKRYINTISYKTLSLSLKELEADNLIIRTEYPQIPPKVEYSLSERGKSLIPILDAMC
                     EWGERNRP"
BASE COUNT         1119 a          403 c          483 g         1025 t
ORIGIN      
        1 aaacatcttc cctatttttt attaaatttt ataataatag tcctattaac gcaaatacta
       61 taaaaagagt ttaactttat ccattaaata aatttaaatt aaaacatata gaaatggaag
      121 gagaacaatt tatgataaaa ctaatcggta tattgatagt tgtaattgga tttatactta
      181 aaattgacac actatttact gtattattag caggagtggc aacaggaatt gttgcaggat
      241 tagattttaa tcaaattttt acaattttag gagattcttt tgtctcaaac agaggtgtat
      301 cattattcat attaacattg ccagtgatcg gtgtattaga aagatatggt ttaaaacaaa
      361 gagctgtttc tttaatagaa aaattaaaaa gacttacaac aggaaaagta cttactatat
      421 acatgatagc tagacaaata gctggtgctc tatcaataag aatgagtgga catcctcaat
      481 ttgtaagacc acttgttaat cctatggcac aagctgctgg tttaagtaat agtgatgagc
      541 taaaagaatc ggatgaagaa gccataaagg cattatctgc tgcatcagaa aactatggaa
      601 atttttatgg tcaaaactta tttgcaggta gtagtggagt attacttata gcatctactc
      661 tgactcaatt tggatacaat gtaactggac ttaatattgt aaaggctagt actattatgg
      721 cagtaatagc acttgtagtg gctacaatcc agtttacctt atatgataaa aaattaaaca
      781 aaagtcataa aaaatagttt tatttaggag gaataaaaat gcaaaatttt ataaacatat
      841 cattagaaat cttttacgca ctaatgggat ttttaatgat tgttatagca tataagtctt
      901 ttactaccat aaataacaat aagaaatatg gtacatcatt attttggata cttatatcac
      961 tgccattcat atttggtaga ttgattccag ccaatataat aggaataata ttaatattat
     1021 ctagtttatt aacactttct aaacaagttg tatttgcaaa gtatgaagaa ccagatgaaa
     1081 attttggaaa agaacaagca gacaaactta aaaataaaat atttatacca tcattaatat
     1141 tagcatttgc tgcagtagta gtagctatga gcctttctaa ttttgataat tcttctcaat
     1201 ttgcaatagg tgtgggttca ataattgcat taatatcagc ccttataata actaaggcaa
     1261 aacctgctac atcagttcaa gatggctcaa gattacttca acaaatggga cctgctagta
     1321 tgttaccaca acttttagtt gcattgggag ctttatttac tcaagccgga gtcggtgaag
     1381 ttatttcaac catgatatct ggtgtagtcc ctgctgatag cagattattt ggtgttatag
     1441 cttatgtatt aggtatggtt atatttacca tgataatggg aaatgcattt gccgcatttt
     1501 cagtcataac ggcaggaatt ggtattcctt ttgtattgtc tcaaggtgga aatcctgcta
     1561 taattggagc attagcatta acagcaggat attgtggaac tctacttact ccaatggctg
     1621 ctaattttaa tatagttcca gcagccttgc tagaatgtaa aaatgattat attgttataa
     1681 aatatcaagc tccagttgct ttggtattaa ttatagccca catattagta atgtactttt
     1741 taggatttta atataattag gaggtaaaat aatgaaaatt ttgttaacag gatttgaccc
     1801 atttggtgga gaaccaataa acccagctca agaagcagtt gaaagggtca ataataatat
     1861 aaatggtgca gaaatcataa agataactat accaacagtt atgacaaaat ctgttgaagc
     1921 tatagataaa gctattcaag agcacaatcc agatatagta atatcagttg gacaagctgg
     1981 aggtagattt gacattacac ctgagagagt tgctataaat atagatgatt ttagaataaa
     2041 agataatgag ggtaaccagg ttatagatac tattataaaa gaagatggtg aacctgcata
     2101 tttttcaaaa ttgcctgtaa aagctatggt aaaacatatg aatgaaaata aaattccagc
     2161 tagtgtttca aacactgcag gaacatttgt ttgcaatcat gtgatgtatg gaattcttta
     2221 tatgatagac aagaaatatc ctaatataag gggtggattt atacacatcc catatacaac
     2281 ttcacaagta atagataaaa aaaatacacc attcatgtct ttagaagaaa tagtaaaagg
     2341 cttagaatta gctatagaag cttgtattat atataaagaa gatgtaaaag aaattggtgg
     2401 tgaaatttca taataaattt tttagtattt ttataaatta aaaatactaa ctattaattt
     2461 aaatatagac tgtgagtaga ttattcaaac aattttactc acagtctttt tatggtatct
     2521 acagatacaa aattaaggtc tattcctctc tccccactca cacattgcat ctaaaattgg
     2581 aatcagagat tttccccttt ctgatagact atattctacc ttaggaggta tttgaggata
     2641 ttcagtacga ataatcaagt tatctgcttc taattctttt aatgataaac tcaaagtttt
     2701 ataggagata gtatttatat aacgttttaa tgcattatat cttacaactt caaattctac
     2761 aagacagtaa agaattatca tcttatattt accactaatc agtgaaagtg tataactaaa
     2821 tcctgtttca cttaaatctg tatttgatat acagtctata ctcatatatt acactctcct
     2881 tttggttagt attgaacaaa aatgtgcgta cttgttttta gaataattta taataaaatt
     2941 atagtgtaag aataattgaa agtcaatttt ataataggag gtgtatttac taaaaaaaaa
     3001 gttattttaa ataggattct aagattagac
//
LOCUS       DAELLV010000321         3023 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.321, whole genome shotgun sequence.
ACCESSION   DAELLV010000321 DAELLV010000000
VERSION     DAELLV010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.321"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(73..597)
                     /locus_tag="KPD58_003489"
     CDS             complement(73..597)
                     /locus_tag="KPD58_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0267214.1"
                     /translation="MKHKYGYLLLESVVSLSSMVIIILVLYSIFLSTINLKLKVEDKI
                     ELQQQSLEIIKSMEGIISNSMGIMNVSNYEETFKKTTSIKCRYLDENNNEEGISNKEI
                     ILNERRNKLFVNSLNGESSQAGGYEIGDYVDEMYVSITNNGQYVNIKLKLSKRSQKYE
                     TDFKIKVWNFSESI"
     gene            complement(587..1006)
                     /locus_tag="KPD58_003490"
     CDS             complement(587..1006)
                     /locus_tag="KPD58_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0267215.1"
                     /translation="MTVIELIVSISIILLIVTLSFPKDNLENHKINLFARQLCSDIRY
                     VRRINMFSNYNTYIYYINQGKHEGYVLRKDGKNIKSIMLPKNAKIARSIEIIKFKSDG
                     SPQKASTIEIYNNRLRKTITITPVSGRVLLKEGKYET"
     gene            complement(1026..1751)
                     /locus_tag="KPD58_003491"
     CDS             complement(1026..1751)
                     /locus_tag="KPD58_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0267216.1"
                     /translation="MLLENKGSILIITLIIFSIISTVCIMCIGLIYTHNNMFELGYKD
                     IQLKEISLSGIEISVSNVLSCVEEAIDVSNNEDEFREYFLGNNMIECIKKIKDTSYSS
                     LEELSLKVENNTIYDRGDFFEFDIESNVREKEFLKAIKVRVKIKNPWIGIDIEDYMDK
                     SNNNKIDDKTFNNYKASENNEILNQFKNESENIRNQEVGGFTEEDEEDEEYEEELKQN
                     IIENIKKNFDEKNLVQVYKYRGV"
     gene            complement(1735..2097)
                     /locus_tag="KPD58_003492"
     CDS             complement(1735..2097)
                     /locus_tag="KPD58_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0267217.1"
                     /translation="MKIRKSGFISIECIISIAILYVAVYLVSTSLYNCYSFISRNISD
                     REMLSTAKKYIEDEKYRIQNSKYELIEDKIEKNYINGYEINSKIEQILDYYQCYEINI
                     EIKNEFKKLRFNSYVTRK"
     gene            2664..>3023
                     /locus_tag="KPD58_003493"
     CDS             2664..>3023
                     /locus_tag="KPD58_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG0267218.1"
                     /translation="MAALKSFEKPLTPEEEIEYLTKFKIENDKSAKDTLIERNMRLVA
                     YIAKKYNNSTEDQDDLISIGTIGLIKAIETYNIDKGTRLATYASRCIENEILMNIRSN
                     KKNKTQVSLQDPIGTDKE"
BASE COUNT         1027 a          483 c          250 g         1263 t
ORIGIN      
        1 ttatatagtt ctcaataatt atattttata tgacttattg atgtcacttc tttctcttaa
       61 atttatgtat ttctatatac tttcactaaa attccatact tttattttga aatcagtctc
      121 atatttttga ctacgttttg ataatttcaa tttaatatta acatattgac cattattagt
      181 aattgataca tacatctcat ctacataatc tccaatttca taaccacctg cttgactact
      241 ttcgccattt aaagaattta caaataactt atttcgtctt tcattcaata taatttcttt
      301 attacttatg ccttcttcat tattattttc atctaaatat ctacatttta tagatgttgt
      361 ctttttaaat gtttcttcat aattagatac attcattatt cccatagaat tgctaataat
      421 tccttccata gattttatga tttcaagact ttgttgttgt agttctatct tatcttctac
      481 ttttaacttt aagtttattg tagacaaaaa tatagaatat aaaactagta taattatcac
      541 cattgaagac aatgatacga cactttccaa aagcaagtaa ccatatttat gtttcatatt
      601 ttccctcttt cagaagtact cttccactta caggtgttat tgttatagtt tttcttaatc
      661 tattattata tatctctatt gtagacgcct tttgaggcga cccatcagat ttaaacttta
      721 ttatttcaat actcctagct atttttgcat ttttaggtag cattattgat ttaatgtttt
      781 taccatcttt tctcaataca taaccttcgt gtttaccttg atttatataa tatatatatg
      841 tattgtagtt gctaaacata ttaattcgcc taacatacct tatatctgaa caaagttgac
      901 gtgcaaataa atttatttta tgattttcaa gattatcttt tggaaaagat aatgtaacta
      961 tcaagagtat tatactgata gatactatta attctattac agtcacattc cttctttttt
     1021 caaagttata cacccctata cttatatacc tgtactaggt ttttttcatc aaaattcttc
     1081 tttatatttt caattatgtt ttgtttaagt tcttcttcat attcttcatc ttcttcatct
     1141 tcttctgtaa atccaccaac ctcttgattc cttatatttt cactttcatt tttaaattga
     1201 tttagaattt cattattttc agatgcttta taattattga atgttttgtc atcaatttta
     1261 ttgttattag atttatccat ataatcttca atgtcaattc ctatccatgg atttttgatt
     1321 ttcactctta cttttattgc ttttaaaaat tctttttctc taacattact ttctatatca
     1381 aattcaaaaa aatctcctct gtcataaata gtattatttt ctacttttaa agaaagttct
     1441 tctaaactac tataagatgt atcttttatt ttttttatac attcaatcat attatttcct
     1501 aagaaatatt ctctaaattc gtcttcatta ttagatacat caatagcttc ctcaacacaa
     1561 gaaagtacat tacttactga tatttctatt ccacttaaag aaatttcttt tagttgaatg
     1621 tccttgtatc ctaactcaaa catattatta tgtgtataaa ttaaacctat acacataatg
     1681 cacacagtac ttataataga aaatattatc aatgttatta ttaatataga gcctttattt
     1741 tctagtaaca tagctattaa acctcagttt tttaaattca ttttttatct ctatatttat
     1801 ttcatagcat tgataataat ctaaaatttg ctctatttta ctgttaattt catatccatt
     1861 tatgtaattt ttttctatct tatcttcaat taactcatac ttactatttt gtattctata
     1921 cttctcatct tctatatatt tttttgctgt acttaacatt tctctgtcag atatatttct
     1981 actgataaaa ctataacaat tatacaatga tgtagaaact aaataaacag ccacatataa
     2041 tatagcaata cttattatac attcaattga tataaaacca ctctttctta ttttcaataa
     2101 taaaaacaac ccctaaataa tattttatat ataaacattt aactataagt tttttcataa
     2161 atatcttttg taagtttttt cattgtaatc aaaatttctt cccatacata tataaattaa
     2221 cacaccacct tcacccttaa cagtattaac tatgactgat acttttgctt gtaatcaact
     2281 ttatacaaac aattaattac atttactttt ttaagtattg taaaattaca cctcctatat
     2341 tcatattttt atagactcta atgctttgca ttaatctttt acttcaacac caaaacatat
     2401 ataaatacta tttcctttta tatttattga cacctcaatt ccaatttata ctttataaat
     2461 caataatttc ttcgccttat ttcatatata tacaacaaaa acttaaattt aaattcccac
     2521 tctttatgtc atattccatt caaaagtttg caaatatatg ttttaaattc taaaaatatg
     2581 catattattt tattagataa cagtcaactt ggataagtct ccttcatata atttaaagtg
     2641 accgttgaaa agggggtgac ctattggcag ctcttaaatc ttttgaaaaa cccttaaccc
     2701 ctgaagaaga gattgagtat ttaactaaat ttaaaataga aaacgataaa agtgcaaaag
     2761 atacattaat tgaaagaaac atgagattag tagcttatat agcaaagaaa tataataatt
     2821 caacggaaga tcaggatgat ttaatatcca taggtacaat aggattaatt aaagctatag
     2881 aaacttataa catagataaa ggaactcgat tagcaacata tgcttctaga tgtattgaaa
     2941 atgagatttt aatgaatatt agatctaaca aaaaaaataa aactcaagta tctctacaag
     3001 accctatcgg aacagataaa gaa
//
LOCUS       DAELLV010000322         3012 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.322, whole genome shotgun sequence.
ACCESSION   DAELLV010000322 DAELLV010000000
VERSION     DAELLV010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.322"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..555)
                     /locus_tag="KPD58_003494"
     CDS             complement(<1..555)
                     /locus_tag="KPD58_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBG0267219.1"
                     /translation="MFNLIRKDLIIGISSDGIRNLKYILLFFVFYFFLNSISYYTVSI
                     VVSYLIFINTFECDYENDSRIFIRSMPVSIEDIVYSKYLLGVGLIISVTIIVSLLSKL
                     TSLVFFRNMVLNDVFFSVNIFLAILTILLPLIFKFGYGKMKVCGFIVSILIYFVYGSL
                     LKMISMIVYQVKHFNYSKVGGVYLS"
     gene            complement(555..1415)
                     /locus_tag="KPD58_003495"
     CDS             complement(555..1415)
                     /locus_tag="KPD58_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0267220.1"
                     /translation="MFSIRNITKKLGKFKLNNINLELKEGDIVGIIGPNGSGKTTLIK
                     IIMGIIDADEGEIELCNETIENSPISFKNNIGFVYDSLQFYPHLKVKEFRKIVSLFYK
                     NFDRERFDEYLNKFDIEENMHIENLSKGQSEKLMLSSALSHNAKLLILDEPTAGIDPI
                     VRTEIMQYLQDFVKNGSSSVIISTHNTDNLIKIADYLVFINRGNQIFTVKKELIEQEY
                     KIIRANKAELEAIKESIVGVKEYKYYNEALVKVGDSLKVKSLLIEIDKHKVKNPTIEE
                     LMYYYVNEVR"
     gene            1550..2350
                     /locus_tag="KPD58_003496"
     CDS             1550..2350
                     /locus_tag="KPD58_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HBG0267221.1"
                     /translation="MKRIITINTNQLNKSLETKELLTRKLINAGFEVYSDIYPDTELI
                     ISIGGDGSFLRTVRDFDFPEIPIMGINTGHLGFFPDILPDKIDSFIEAYTKKDYIIQE
                     MSLLNAEVYTTTSGSNMLAVNEVVIRGDKSRTIHLNLSLDNKHIQNFSGDGMIISTST
                     GSTAYNYSAGGSIVDINLELMQITPLHPINTNAYRCFTSSIICSNESVIKIAPEYRFE
                     DSVLIVVDGVEHRFRQIENIKVSISDAKIKLLRMSNYEFWHRVSEKFL"
     gene            2375..2944
                     /gene="yfcE"
                     /locus_tag="KPD58_003497"
     CDS             2375..2944
                     /gene="yfcE"
                     /locus_tag="KPD58_003497"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HBG0267222.1"
                     /translation="MKIGIMSDTHGSLLYFEKALNVLSDCDVLLHGGDVLYHGPRNDI
                     PEGYNPKKFIETLNKLENIVIVKGNCDADVDQMVIEHPIQSPYVMSQFGEIRIILNHG
                     YIESEEEIIDKAKKMGGDILVLGHTHVKKLYMDDNLIVINPGSTSIPKDGSHSVAIID
                     IIKTDDEDELELDINLIDINTGNIININD"
BASE COUNT         1128 a          448 c          370 g         1066 t
ORIGIN      
        1 tgaaagatat actcctccaa ctttactata attaaaatgc tttacttgat atacaatcat
       61 actaatcatc ttcaaaagac ttccatatac aaagtaaatt aaaatactta ctataaaacc
      121 acaaactttc atttttccat atccaaactt aaaaattaaa ggcagtaaaa tcgtcaaaat
      181 tgctaaaaat atatttacag aaaaaaatac atcatttaaa accatatttc taaagaacac
      241 tagtgatgtc aatttactta gtaaggacac aattattgtt acagaaatta ttaatcctac
      301 acctaataaa tattttgagt aaactatatc ttctatacta actggcatac tccttataaa
      361 aatccttgaa tcattttcat aatcacactc aaacgtattt ataaatatca aataactaac
      421 aacaatactt actgtataat atgaaattga attaaggaaa aaataaaaca caaaaaatag
      481 caatatgtac tttaaattac ggataccatc tgaactaatc cctattatta aatcttttct
      541 tattaaattg aacatcatct cacctcattt acataataat acattagttc ttctatagta
      601 ggatttttga ccttatgctt atcaatttct atcaataggc tttttacttt caagctatct
      661 ccaactttaa caagtgcctc attataatat ttatactctt tcacaccaac tatgctttct
      721 ttaattgctt ctaactctgc tttatttgcc ctaattatct tatattcttg ttctattaat
      781 tctttcttaa cagtaaaaat ctgatttcct ctatttataa aaacaagata atcagcaatt
      841 ttaattaaat tatcagtatt atgagtagaa attattacac tactacttcc atttttcaca
      901 aaatcttgta aatactgcat tatttcagtt cttacaattg ggtctatacc agcagttggt
      961 tcatccaaaa tcaataactt agcattgtga ctaagtgcgc ttgataacat aagcttttca
     1021 gattgacctt tagataaatt ttcaatatgc atattttctt ctatatcaaa cttattaagg
     1081 tattcatcaa acctttctct atcaaaattc ttataaaata aagaaactat ttttctaaat
     1141 tccttaacct ttaaatgcgg ataaaattgt aaagaatcat atacaaaacc aatattgttt
     1201 ttaaagctta ttggagaatt ttcaatagtt tcattacaaa gttcaatttc accttcatct
     1261 gcatcaataa tccccattat aatttttatt aaagtcgttt ttcccgaacc atttggtcct
     1321 ataatcccaa caatatcccc ttcctttaac tctaagttta tattatttaa cttgaatttt
     1381 cctaattttt ttgtgatatt ccttatgcta aacacatctt cacctcttat gcattttttt
     1441 atacccacct atacatattt taacacattc attacaaagg tcatttataa atttataaat
     1501 taagaattat aaaactatgt tataatacaa agtatgaaga ggtgaatata tgaaaagaat
     1561 tataactata aatacaaacc aattaaataa gtcccttgag actaaagaac tcttaactag
     1621 aaaacttatc aatgctggct ttgaagtgta ttcagatatt tatccagaca cagagcttat
     1681 tatatcaatt ggtggtgatg gctctttttt aagaacagtt agagatttcg attttcctga
     1741 gattcccata atgggaataa atacaggtca tctggggttt tttcctgata ttttaccaga
     1801 taagattgat agctttattg aagcttatac aaaaaaagat tatattattc aagaaatgtc
     1861 acttcttaat gcggaagtat atactactac tagtggttct aatatgcttg ctgtaaatga
     1921 ggttgttata agaggagaca agtctaggac tatacaccta aatttaagcc ttgataataa
     1981 acatatacaa aattttagtg gagatggtat gattatttct acttccacag gttcaacagc
     2041 ctataactat tctgctggag gtagtattgt tgatataaat cttgaactta tgcagattac
     2101 acctttacat ccgataaata ccaatgcata taggtgcttt acatcaagta taatatgttc
     2161 aaatgaatct gttataaaga ttgcaccaga atatagattt gaagattctg tattgattgt
     2221 cgttgatgga gttgagcaca gatttagaca aatcgaaaat ataaaagtta gtatatctga
     2281 tgcaaaaata aaattactta gaatgtcaaa ctatgagttt tggcatagag tatctgaaaa
     2341 atttttatag actgtactta ggaggatttt taatatgaaa ataggaataa tgagtgatac
     2401 tcatggtagc ttactttact ttgaaaaagc gttaaatgta ttatctgatt gtgatgtttt
     2461 attacatggt ggagatgtct tatatcatgg acctagaaat gatattccag aaggatataa
     2521 tcctaaaaaa tttattgaga cattaaataa attagaaaac attgtgattg taaaagggaa
     2581 ctgtgatgca gatgttgacc agatggttat agaacatcct attcagagtc cttatgtcat
     2641 gagccaattt ggtgaaatta gaattatatt gaatcatggt tatatagaat cagaagaaga
     2701 aattattgac aaagcaaaga aaatgggtgg tgacatatta gttttaggac atactcatgt
     2761 aaaaaaacta tacatggatg ataatttaat agtaataaat ccaggaagta cttctatccc
     2821 aaaagatgga agccattctg tcgcaattat agatatcata aaaactgatg atgaggatga
     2881 gcttgaactt gatataaact taattgacat caatactgga aatataataa atatcaatga
     2941 ttagtaaatt tttaataaaa ataaaataag ctttaattta ttaaactcat aataaaaaca
     3001 agtaaaccat tt
//
LOCUS       DAELLV010000323         2946 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.323, whole genome shotgun sequence.
ACCESSION   DAELLV010000323 DAELLV010000000
VERSION     DAELLV010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.323"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            1..1338
                     /locus_tag="KPD58_003498"
                     /pseudo
     CDS             1..1338
                     /locus_tag="KPD58_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816417.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1353..2225
                     /locus_tag="KPD58_003499"
     CDS             1353..2225
                     /locus_tag="KPD58_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0267223.1"
                     /translation="MINKLEIRNLNFNYKNKKALDNINLTLTDGVVALLGPNGAGKST
                     LMRLLVTLYETSIGEIELNNIKYSKNNEKIKANVGYVPQDFDMYNNINGQEYLEFVAK
                     MRGVSKNDLKKHIQKVVSKVNLDKFINKKIGTYSGGVKRRLGIAQALIGDSKLIVMDE
                     PTVGLDPEQRNEFRRLLPEISKNSIVLISTHIVEDIQFNCDKLIILNQGKILYDGTIN
                     KFIDMVEVYSVVVSNDEFEYLERNIDIIDYRRVKDGVQVKYIGKNTEKLKDSLRIDTT
                     LQDAYINFLHKELK"
     gene            2226..>2946
                     /locus_tag="KPD58_003500"
     CDS             2226..>2946
                     /locus_tag="KPD58_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0267224.1"
                     /translation="MCILELIKFELKKIELKHVLIFVIISILCIFITGILDYNELIFY
                     NDVPIMDTYSIAGWLGIIKISDVFIPIMSAITIIYVFSKDYSGNINEILTLYNKKKYN
                     QFIVFRWCVILAIYTVLLLISITIVYTFTSVKGFNIDKIDFVYSVKPIDVFLKTFPTL
                     LWYTTFPLMILTLTKNRFTTIAILITYTFADIFFILHMYPFVSMINVNGFYIQKMFYM
                     PNNIDVRFNELKNYFFINRGSL"
BASE COUNT         1221 a          257 c          447 g         1021 t
ORIGIN      
        1 tgtaacgatt aaatcaacta tatttatatt gatatttgaa tggtttttac cacttactat
       61 tttatctata gtgatatcat ttatttcagt tattataaat aatttaatac taaagattac
      121 cttatcatca gtatttttat atttaacatc aactaatatg ttggatagag ttattgatat
      181 taaagaacca ttttttaaag cattatttaa atcacttaac atatttgaag atcaatcata
      241 tgcttactat tctgattttg tagggaaagt taataattca gcatatgtta tagataagat
      301 tatacctaca ctatttgcaa ttactttaat aatagtagca tatctgatat taaatgatat
      361 tagaaagaaa cagattaaat cattaatagt tatagtagtg tacagtattt tatttattgg
      421 attaaattat atatcaatag atatgtcaac atatagagat gaggagcgta gttatggaga
      481 gtatttaaag aaatctaata ttagtgatat taaaataaaa tcgcatgata tgaagattga
      541 ctttaaaagc aagtcaaata taaaagatag aattgtttta ttaaacacat caaatgaaga
      601 gttaacaaat attaatatga ttttagatga aatatttgat ataaaatata taaaaataaa
      661 ttcggaaaat ataaaattta acaaaaaaag gatttaattg aagttaattt aaaggaaagt
      721 ttagagccta atgacgaggt gacattagat atagagtatg aaggctatat taatgtgttg
      781 acatcttgga atcaaaataa atatatagct actaaagatg atattatgct tccaccaggt
      841 tctttagcgt ggtacccaaa agtaaatcaa tcaagttgta tggcttataa aataaattta
      901 agttctaaaa ataatgtcta ttcaaattta cctttactga ataagactag taatttttta
      961 aaaaaagatt atatatttga aggaaagtca aatgatattg caatatatgc aggtgagttt
     1021 aaagaagagg taaaagatga tataaaaata atctatccat cagatagtaa tgtaaatatg
     1081 gatatagagg aaaaaattaa atttttaatt gaaacaagaa atgaaatatt aggtgatagc
     1141 aaagcatctg tagatgctaa aaaatatgta gatgaagtag ataatgttat ggaatcaata
     1201 aaaagaaaaa aaattaatat gatattagtt gctgacttac ctagcaatga atttttatat
     1261 gaagactcaa agggagagaa aattagcata actatttatc attggctttc aggaaatatc
     1321 cttataatag ataaataata aaggaggtta atatgataaa caagttagaa ataagaaatc
     1381 taaattttaa ttacaaaaat aaaaaagctc tggataatat aaatttaacc ctaacagatg
     1441 gagtagttgc attattaggt cctaatggtg ctggaaaatc aacattaatg aggttattag
     1501 tgactcttta tgaaacttca attggagaga tagaattaaa taatataaag tatagcaaaa
     1561 acaatgagaa aatcaaggca aatgtaggat atgtacctca agattttgat atgtataata
     1621 atataaatgg gcaagaatac ttagaatttg tagctaaaat gagaggtgta tctaagaatg
     1681 atttaaagaa acatatacaa aaagtagtat caaaagttaa tctggacaag tttataaata
     1741 aaaaaatagg tacatactca ggtggtgtaa agagacgact aggtatagca caggctttaa
     1801 taggtgattc taagttaata gttatggacg aacctacagt tggtcttgat ccagagcaaa
     1861 ggaatgaatt tagaagattg ttaccagaaa taagtaaaaa ttctatagta ttaatttcaa
     1921 ctcacatagt agaagacata caatttaact gtgacaaatt aattatatta aatcaaggca
     1981 aaatattata tgatggtaca attaataagt ttattgatat ggttgaggtg tattcggtag
     2041 tagtttctaa tgatgaattt gaatatttag aacgtaatat tgatattatt gattatagga
     2101 gagttaaaga tggtgtgcaa gtaaagtata taggtaaaaa tacagagaaa cttaaagata
     2161 gcttaaggat agatacaact ttacaagatg cttatataaa ctttttacat aaggagttaa
     2221 aataaatgtg tatattggag ttaataaaat ttgagcttaa aaaaatagag cttaaacatg
     2281 tgttaatatt tgtaattatt tctatattat gcatatttat tacaggcata ttagattata
     2341 atgagttaat attttataat gatgtaccta taatggatac atattctata gcaggatggt
     2401 taggaattat aaagatatca gatgttttta taccaataat gtcagcaatc acaataattt
     2461 atgtgttttc aaaagattat tcgggtaata taaatgagat acttacttta tataataaaa
     2521 aaaaatataa tcaatttata gtatttagat ggtgtgttat attagctata tatactgtat
     2581 tattattaat ttcaattaca atagtatata catttacgtc agtgaaaggt tttaacatag
     2641 acaaaataga ttttgtttat agtgtaaagc caatagatgt gtttttaaag acgtttccta
     2701 cattattatg gtatacaaca tttccactaa tgatattaac attaacaaaa aatagattta
     2761 caacaatagc aatattaatt acatatacat ttgctgatat attttttatt ttacatatgt
     2821 acccatttgt tagtatgatt aatgtaaatg gattttatat tcaaaagatg ttttatatgc
     2881 ctaacaatat agatgttaga tttaatgaat taaagaatta tttctttata aatagaggtt
     2941 ctctag
//
LOCUS       DAELLV010000324         2942 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.324, whole genome shotgun sequence.
ACCESSION   DAELLV010000324 DAELLV010000000
VERSION     DAELLV010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.324"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            481..2292
                     /locus_tag="KPD58_003501"
     CDS             481..2292
                     /locus_tag="KPD58_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBG0267225.1"
                     /translation="MKKKYDFLIYSAIWLVIIILFIFSLYHSIEHIKIISHTGTIRSE
                     TQKVVKQELNNERNDDLIKRLDNILIKLRTGNGENGFQRCDNKEFQQKLNQMDSMWES
                     MKKEIIKVRNGASGDKLYKLSEEYSVLSNQIVFISEKHSNAKLYSFATALFIYLIFST
                     ISLLIWEYYNKKRFKRIFYTDNLTKIKNQVAFENRAIEILHNASNKEYVLLNIDIDNF
                     KYINDTHGYEYGDKVLIIVAAALSKTFNIKETCARIGSDNFVILAKYRDSLLEDIREM
                     LTNAIISELDMNVTQTISYCIGAYLVEIDNLGYKSINSIMDKANIAHKVSKTRGISST
                     VWYNENLLKQLQMENSIYNYMYKAIENEEFHMYLQPKFQISSLNVVSAEALVRWFSPE
                     LGFLSPDEFIPLFEKSGFIIELDFYMLKKACSFVRKTFMKKNQYTYPIAVNFSRVTIY
                     QNSFYQRFLDTVKEYEIPFKYIEIEVTESAFNEISQPVISILEELKKLGFLISMDDFG
                     SGYSSLSLLCSLSINGLKLDKSLLKETFNREKVYSIIQCIIEMSHRIGMSVVCEGIET
                     KKDLEFLNTVKCDVGQGFYFSKPIEEKEFFNKYVTKK"
     gene            complement(2380..>2942)
                     /locus_tag="KPD58_003502"
     CDS             complement(2380..>2942)
                     /locus_tag="KPD58_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0267226.1"
                     /translation="NQFEKSYDIGIFKNEQSIYKFIAKIPFVKKDNSNFNYTNYYMEF
                     EDIPEYYEVNFNNYLYIITRFSFIPDGTEIFFTWEEIHCWDEKVATEKTFINGETLVD
                     SYNFPNNEVKDYIQKRESLYIETKKYYEEKGLKIERHALGSEDGEYVELTNDSILYLL
                     DIEAVEIREQSKNIEEFLYKYKKRFK"
BASE COUNT         1127 a          300 c          414 g         1101 t
ORIGIN      
        1 aaataagtaa attattggtc ttattacaca aaaatagata taatttaagt aatataccaa
       61 aaaaaaggaa tatatttaca taatatgata aaataaattc aaagagttaa attgtaggta
      121 aatagtattt taattattta ttaaaatata gattatgtat atgccaaatt taaaaagaga
      181 cataaagaca ttatacaaat atattattgg ggtgatattt tataagaaaa gaagtgatgc
      241 agtaaattaa ttgagcaatt taaaaatgat ttaatgatag tgttgatgaa tgtttaaaaa
      301 gaaattttag aaaaacgtat attgctgttt gtgttggagg tgatgaattt gtataattta
      361 taggatataa atgattaaac tatttaagat aaatacatat cactgtaaaa tattgataat
      421 cattttttat aagagattga ttatttgcaa attatatata ttacaaatga ggtaacaata
      481 atgaagaaaa aatatgattt tttaatttat tctgctattt ggctagttat tattattctt
      541 tttatatttt ctctatatca cagtatagag catattaaaa ttataagtca cactggtact
      601 attcgcagtg aaactcaaaa ggttgttaag caagaattga ataatgaaag aaatgatgac
      661 ttgataaaac gactggataa tattctgata aagctaagaa caggtaatgg agaaaatgga
      721 tttcaaagat gtgataataa agaatttcaa caaaaactaa atcaaatgga ttctatgtgg
      781 gaatctatga agaaagaaat tataaaagtt agaaatggtg cttcaggtga taaattatat
      841 aaattgagtg aagagtattc tgtgttgtct aatcaaattg tttttatatc tgaaaaacac
      901 tctaatgcaa aattgtattc ttttgctact gcactattta tttatcttat attctcaaca
      961 ataagtcttt taatttggga atattataat aaaaaaaggt ttaaaagaat attttatact
     1021 gataatttaa caaaaataaa aaatcaggtt gcatttgaaa acagagcgat tgaaattttg
     1081 cacaatgcat ctaacaaaga gtatgtactg ctaaatattg atattgacaa ttttaaatat
     1141 attaatgata cacatggata tgaatatgga gataaagttc taataatagt tgcagctgca
     1201 ctttctaaaa cttttaatat caaagaaact tgtgcaagaa ttggttcaga taattttgta
     1261 atacttgcaa aatatagaga ttctctttta gaggatattc gggaaatgct aactaatgct
     1321 ataatctctg aattagatat gaatgtaact caaactatat cttattgtat aggagcgtac
     1381 ttagttgaaa ttgataattt aggttataaa tccatcaatt ctataatgga taaggcaaat
     1441 attgcacaca aggtaagtaa aacaagagga atttcctcca cagtatggta taatgaaaat
     1501 ctgttaaaac agttacaaat ggaaaatagt atttataatt atatgtataa agcgatagaa
     1561 aatgaagaat ttcatatgta tttgcaacct aaatttcaaa tatcttcttt aaatgttgta
     1621 agcgcagaag ctcttgtaag atggttttct ccagaacttg gttttttatc accagatgaa
     1681 tttattccat tgtttgaaaa aagtggattt attattgaat tggactttta tatgttaaaa
     1741 aaagcgtgtt catttgtaag aaaaacgttt atgaaaaaaa atcaatatac atatccgatt
     1801 gcagttaatt tttcaagggt tactatttac caaaatagtt tttatcaaag gtttttagat
     1861 actgttaaag aatatgaaat tccttttaag tatatagaga tagaggtaac tgaaagtgct
     1921 tttaatgaaa tatctcaacc agttatttca attttagagg aacttaaaaa gttaggattt
     1981 ttaatttcaa tggatgattt tggttcagga tactcttcat taagtctttt atgttctcta
     2041 tctattaatg gattgaagtt agataaaagc ttacttaaag aaacctttaa ccgggagaaa
     2101 gtatatagta ttattcaatg tattattgaa atgtcacatc gtataggcat gtctgttgta
     2161 tgtgaaggta ttgaaactaa aaaagacttg gaatttttaa atacagttaa atgtgatgta
     2221 ggacaagggt tttatttttc taaaccgata gaagaaaaag aattttttaa caaatatgta
     2281 actaaaaaat aatataatta tttttatcag tctatacatt gagtcatttt atatctcgtt
     2341 aaatgactca atgtactgtt atgtatttta aaatataaat tatttaaaac gttttttata
     2401 cttatataga aattcttcta tattttttga ttgttctctt atttcaactg cttctatatc
     2461 tagtaaataa agtatagaat catttgtaag ttcaacatat tcaccatctt ctgaaccgag
     2521 tgcatgtctc tcaattttta atcctttttc ttcataatat ttttttgttt ctatatataa
     2581 tgattctcgc ttttgtatgt agtctttcac ttcattattt ggaaagttat atgaatccac
     2641 taatgtttcg ccatttataa aagttttctc ggtagcaact ttttcatccc aacaatggat
     2701 ttcttcccaa gtaaaaaata tctcagttcc atctggaatg aatgaaaacc tggtaataat
     2761 atataaataa ttattgaaat ttacttcata atattcagga atatcttcaa attccatata
     2821 gtaatttgta taattaaaat tactattatc tttttttaca aagggtattt ttgcaataaa
     2881 tttatatatt gattgttcat ttttaaaaat tcctatatca taactttttt caaattgatt
     2941 aa
//
LOCUS       DAELLV010000325         2936 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.325, whole genome shotgun sequence.
ACCESSION   DAELLV010000325 DAELLV010000000
VERSION     DAELLV010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.325"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            97..552
                     /locus_tag="KPD58_003503"
     CDS             97..552
                     /locus_tag="KPD58_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HBG0267227.1"
                     /translation="MDEIDLKIINSLKKNSRASTSEISRQINLSVPAVSERIRKIEQS
                     NLIQKYTIQINREKTKYKLLAFIFINTDLSKSMKEFKKTILEYDTVLECHHISGEYDY
                     LIKVLAEDTSNLEEFISVTLKEIGGIQKANTTIVLSTIKEEFNTIKDIF"
     gene            601..1185
                     /locus_tag="KPD58_003504"
     CDS             601..1185
                     /locus_tag="KPD58_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HBG0267228.1"
                     /translation="MKVLVFLAKGFETMEFSVFVDVMGWARNDYGHDIDVVTCGFKKQ
                     VMSTFNIQVLVDKTIEEVCVDDYDALAIPGGFEEFGFYDEAYDSSFLNLIREFNSKEK
                     IIASICVAALPVGKSGVLKNRKATTYHLKNGKRQRQLSEFDVNVVNEPIVVDKNIITS
                     YCPETAPHVAFKLLEMLTSKEQMDEVKLAMGFKL"
     gene            complement(1190..1513)
                     /locus_tag="KPD58_003505"
     CDS             complement(1190..1513)
                     /locus_tag="KPD58_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0267229.1"
                     /translation="MKKCLDNYSCPIEATLALIGGKYKTLILWHLKDTILRFNELKKL
                     IPKATPKMLTQQLRELESDGLIIRVVYPVVPPKVEYSLSDFGKSIIPILDSMCDWGSD
                     YLENL"
     gene            1665..2183
                     /locus_tag="KPD58_003506"
     CDS             1665..2183
                     /locus_tag="KPD58_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG0267230.1"
                     /translation="MNFVELAKKRYSCRNYQDRKVEKEKLEKVLDVARIAPTGGNRQP
                     QRLIVIQEKEGINKLSKAANIYDAPLAILVCGDKDKVWTRPFDGKQLTDIDTSIVTDH
                     MMLQATELGLASVWVCYFNPDIIREEFSLPDNLEPINILLMGYESKIPESPERHEKTR
                     VPLSEIVSYETL"
     gene            complement(2359..>2936)
                     /locus_tag="KPD58_003507"
     CDS             complement(2359..>2936)
                     /locus_tag="KPD58_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0267231.1"
                     /translation="ENFLVLKNSTIKNIIEQHPEYNRKEKIKTEPVSSYIKYYKKFKK
                     TYPVLLQLESILLKSKGIPDVGVTIESMFLAELKNLLLTAGHDLDKMELPLKIELANG
                     SEHFYGIGGKEHILTKDDLFLSDNIGILSSILNGPDNRTAITKDTKNIMYFVYGPDKI
                     SEKQIQDHLNDIKHYISSAFPDLKVNSIDIF"
BASE COUNT         1089 a          334 c          471 g         1042 t
ORIGIN      
        1 atatttgtat taaattttat tgaaatgatt aaatttattt acttaatgta aattattagc
       61 taaaattgta ttaaataatt aaggaggtga gtagctctgg atgaaataga tttaaaaata
      121 attaattctc taaaaaagaa tagtagggct tcaacctctg aaataagcag acaaattaat
      181 ttatcagtac cagcagtctc agagcgtata aggaaaattg agcagtcaaa tttaattcaa
      241 aagtacacta ttcaaattaa tcgggaaaaa acaaaatata agcttttggc ttttatattt
      301 atcaatactg atttatctaa aagtatgaaa gaatttaaga aaactatatt ggaatatgat
      361 actgtattag aatgtcatca tatttcagga gagtatgatt acttaataaa agtattagca
      421 gaagatacaa gtaatcttga agagtttata tcagtgactt tgaaggaaat tggggggatt
      481 caaaaagcta atacaaccat tgttttatct acaattaaag aagaatttaa tacaataaaa
      541 gatatttttt aaataattta ctagtaaagt gttaattata ttttaggagg aaaatagaat
      601 atgaaagttt tagttttttt agcaaaaggt tttgaaacta tggaatttag tgtatttgta
      661 gatgttatgg gatgggctcg taacgattat gggcatgata ttgatgtagt gacttgtgga
      721 tttaaaaaac aagttatgag cacatttaat atacaagtat tagttgataa gacgatagaa
      781 gaagtatgtg ttgatgatta tgatgctcta gctattcctg gaggatttga agaatttgga
      841 ttttatgatg aagcatatga ttcatctttt ttaaatctaa taagggagtt taattcaaaa
      901 gaaaaaataa tcgcttctat ttgtgtggca gcattgccag ttggaaaaag tggtgtttta
      961 aaaaatcgta aagctactac atatcatttg aaaaatggta aaagacaaag acagttaagt
     1021 gagtttgatg taaatgtagt taatgaacca attgttgtag ataaaaatat tataacctca
     1081 tattgtccag aaacagctcc acatgtagct tttaagcttt tagaaatgtt gacatctaaa
     1141 gaacaaatgg atgaagttaa attagcaatg ggatttaaac tgtaatggtt tatagatttt
     1201 ctaagtaatc agaaccccag tcacacatgg aatctaaaat tggaataata ctctttccaa
     1261 aatcagatag cgagtattct acttttggag gaacaacagg ataaaccact cttataatta
     1321 aaccatctga ttctaattca cgtaattgtt gagttaacat cttaggcgtt gctttaggaa
     1381 ttaatttttt taattcattg aacctcaata tagtgtcttt aagatgccaa agtataagtg
     1441 tcttatattt accaccaatc aatgcaagtg ttgcttctat tgggcaactg taattatcta
     1501 aacatttttt catttttggg ctcctttatt tattttaaag tatcttttta ggtactatat
     1561 tacaaaaaag tgcattattg taatatagta ccatagtgat aaaattatag cataggttaa
     1621 ataaatcatc aacttagcct aataaaacaa tagggggtaa ataaatgaat ttcgttgaac
     1681 ttgcaaaaaa aagatattct tgccgtaatt atcaagatag aaaggttgaa aaagaaaaac
     1741 ttgagaaggt tttagatgtt gcaagaatag ctccaactgg tggtaatcgt caacctcaaa
     1801 gattgattgt tattcaggaa aaagaaggta taaataaact ttctaaggct gccaatatat
     1861 atgatgcgcc attagcaatt cttgtatgtg gagataaaga caaagtttgg acaagacctt
     1921 ttgatgggaa acaacttaca gatattgaca caagtatagt tacagaccat atgatgttac
     1981 aggcaactga acttggattg gcaagtgttt gggtgtgcta ttttaatcca gatataatta
     2041 gagaagagtt tagtttgcca gataatctag aaccaataaa tatattattg atgggatatg
     2101 aatctaagat accagagagc ccagagagac atgaaaaaac tcgtgttcct ttaagtgaaa
     2161 ttgtttctta tgaaactctt taattataaa attaagttat tcataggata aatgtaataa
     2221 agtgtttgtt ataaacaaaa ttcaaaataa ataatatcta catataatta attttagtga
     2281 agtatatcta gtttaactag aatgtatact tcactaattt atttattcaa aatatttatg
     2341 aggattttaa tatattaact aaaatatatc aattgaatta acttttaaat ctggaaaagc
     2401 acttgaaata tagtgtttta tatcatttaa atggtcttga atttgctttt ctgatatttt
     2461 atcaggtcca tatacaaaat acataatatt tttagtatct tttgttatag ctgttcgatt
     2521 atcagggcca ttaagaatac tagataatat accaatatta tctgataaaa ataaatcatc
     2581 ttttgttaaa atatgttctt taccacctat accataaaaa tgttcacttc catttgctaa
     2641 ttctattttc aaaggtaatt ccattttatc aaggtcatga cctgctgtta gaagaagatt
     2701 tttcaattct gctaaaaaca ttgactcaat tgttacacct acatctggga taccttttga
     2761 ttttaataga atagattcta attgaagcaa tacagggtaa gttttcttga attttttata
     2821 atactttata taacttgaaa caggctctgt tttaattttt tctttgcgat tgtattctgg
     2881 gtgttgttct ataatatttt taattgtaga gtttttaaga actaaaaaat tttctt
//
LOCUS       DAELLV010000326         2929 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.326, whole genome shotgun sequence.
ACCESSION   DAELLV010000326 DAELLV010000000
VERSION     DAELLV010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.326"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            35..1144
                     /locus_tag="KPD58_003508"
     CDS             35..1144
                     /locus_tag="KPD58_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG0267232.1"
                     /translation="MDFKSLGISENTINILKKLGITTPTPIQKESIKLIKEGKDVIAE
                     AQTGTGKTLAFLLPIFENISLDINDIQVLILSPTRELAIQITEEAMKLKESKDVSILA
                     AYGGKDIGSQIKKLKGNIHMIIATPGRLLDHLNRKTIDLSKLKTFVLDEADQMLLMGF
                     KNEVEAILKETSNKKQTLCFSATINSQVKKLAYRYTKNPVVVSIQKEEITLNNIKQEV
                     VETTDRKKLDALCKVLDEDNPFMAIIFCRTKRRVDHLEEALAIRGYNCQKLHSDIAQS
                     KRERIMKSFRSLDIQYLIATDVASRGLDISGVSHIYNYDLPETPEDYIHRIGRTGRAG
                     EEGYTCAFIDPKNERMLSEIETAIESKISRRIIEL"
     gene            complement(1521..2705)
                     /locus_tag="KPD58_003509"
     CDS             complement(1521..2705)
                     /locus_tag="KPD58_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0267233.1"
                     /translation="MQKSVKKAKVTGSMLAIFGVASVLFSSHAGGGFATGNQETQYYV
                     QYGWTAPLMAILAMIILTATMREVIIMYNNNNCRNYKDLFCELWRPYPKLEIIWEIYY
                     YLMVLIAVSAVIAGAAAVFQSIGVNYFVAVFIIGVVLLVFTIFGAMLVSKAATAMTIA
                     ILVCTLTIFIVGIKAKVPEITEILSNRTSFTPGYIKPILNTFIYAGFQSVVIPTLAGC
                     SRPLLKNSKEATKAMIISFVMNAIALGLAVTMLMGWYREIIAAGQTTLPTLYVAGQSG
                     NHTIYIIYNVALFLCLMSTGVTTIFGLVNRFEDHKALSFLSSRMKRRVFTACAIMVVS
                     MLISLTGLSNIVKYGYGYCGYLGLFTIVIPFLTLGHYKNKKFAKENPEAKWPAELNEN
                     VN"
BASE COUNT         1107 a          444 c          405 g          973 t
ORIGIN      
        1 aaaaagaaaa tttttcatga aaggtaagat aacaatggat ttcaaaagct tagggattag
       61 tgaaaataca ataaatattc taaaaaaatt aggaataaca acacctacac ctatacaaaa
      121 agaaagtatt aaactaataa aagaaggaaa agatgtaata gcagaagctc aaactggaac
      181 aggaaaaaca ctagcttttt tacttccgat atttgaaaat atatcattag atataaatga
      241 tatacaagta cttatacttt ctcctacaag agaactagct attcaaataa cagaagaagc
      301 tatgaaactt aaagaatcta aagatgtaag tattcttgca gcatatggag gaaaagatat
      361 tggttctcaa ataaagaaac taaaaggaaa tattcatatg attatagcta caccaggtag
      421 gcttttagac catctaaata gaaaaacaat agaccttagt aagttgaaga cttttgtttt
      481 agatgaagca gaccaaatgt tgcttatggg atttaaaaat gaagtggaag ctattttaaa
      541 agagacttca aacaaaaagc aaacactatg cttttctgca actataaatt ctcaagtaaa
      601 aaaattagct tatagataca caaaaaatcc tgttgtagtt tctattcaaa aagaagaaat
      661 cactcttaat aatataaaac aagaagtagt agaaactaca gatagaaaaa aattagatgc
      721 cttatgtaag gtactagatg aagataatcc ttttatggca ataatatttt gtagaactaa
      781 gagaagggtt gaccatcttg aagaagctct tgctataaga ggatataatt gtcaaaaact
      841 tcatagtgat attgctcaat caaaacgaga aagaataatg aaatcattta gaagtttaga
      901 tatacaatac ttaatagcta cagatgtagc ttctcgtggt cttgatatat ctggagtaag
      961 tcatatatat aattacgatt taccagaaac accagaagac tacatccacc gtattggcag
     1021 aactggtaga gctggtgaag aaggatatac atgtgctttt attgacccta aaaatgaacg
     1081 aatgttaagt gaaatagaga cggctataga atctaaaatt agtagaagaa ttatagaatt
     1141 ataatttata gattgcttaa aataccaatg ccacacaaac actgtaatat atagaaagct
     1201 acagaaaaca tgtatcaatc tatactttgc tgttatacta ttttatatta aagaaaaaag
     1261 aggctatcca aagtaggatt agtctctttt attgtcaaaa taatgaaaat atacttgact
     1321 tcatataaaa aagagtgcct tataaactaa aagtccattt tgagacaccc tctttttatc
     1381 ttttattctt agttttatat tgcttgttta tagttgttta atattagatt ttgcaaataa
     1441 tcttaataaa catgcagata tattatctat agtattctta tctatagtat tctctttagc
     1501 aatatttttt gctactacat ctaatttaca ttttcattta attccgctgg ccatttagct
     1561 tctggatttt cctttgcaaa cttcttattc ttataatgtc ctagtgtaag gaatggaatt
     1621 actatagtga atagaccaag ataaccacaa tatccatatc catatttaac aatattactt
     1681 aatcctgtaa gtgatataag cattgacact accattattg cacaagctgt aaataccctt
     1741 cttttcattc ttgaagaaag gaaagaaagt gctttatggt cttcaaatct atttacaaga
     1801 ccaaatatag tagttactcc tgtagacatt aaacaaagga ataatgcaac attataaatt
     1861 atgtatatag tatgatttcc agattgacct gcaacataaa gagtagggag tgttgtttgc
     1921 ccagcagcaa taatttctct ataccatccc attagcatag ttacagcaag tcctaatgca
     1981 attgcattca taacaaatga aattatcatt gccttagtag cttcttttga gttcttaaga
     2041 agtggtcttg aacatcctgc aagtgtaggt ataacaaccg attgaaaacc tgcataaata
     2101 aaagtattaa gtattggctt gatatagcca ggtgtgaaac tcgttctatt tgataatatt
     2161 tcagttattt ctggtacttt agcttttata ccaacaataa aaatagtaag agtacatact
     2221 agaatagcta ttgtcattgc tgtagctgct ttagatacaa gcatagcacc aaatatagta
     2281 aagacaagaa gtacaactcc aataataaat acagctacaa aataatttac tcctatactt
     2341 tgaaatactg ctgctgcacc tgctataaca gcacttacag ctattaaaac cattagatag
     2401 taatatatct cccatattat ttcaagtttt ggatatggtc tccaaagctc acaaaataaa
     2461 tccttataat tcctacaatt attattatta tacattatga taacttctct catggtagca
     2521 gtaagtataa tcattgctaa aattgccata agtggtgctg tccatccata ctgcacatag
     2581 tactgagttt cttgattccc tgtagcaaat cctcctcctg catgtgaact aaaaagaacg
     2641 cttgcaacac caaaaatggc taacatagag cccgttactt ttgctttctt tacacttttt
     2701 tgcatgaaat catcctccct aaaatgatat taaaagtttt tattttaaca ctttattgat
     2761 aaaacaataa catttttgct cattaaattc aactatattt ttccaatgac tttacatttt
     2821 caacttattt ctacctttat attaaatatt ttttatatat ttattttatt ttttaaatag
     2881 tattcaaatt cactttgttt tttaatttta tgaaatatct tttttaatg
//
LOCUS       DAELLV010000327         2918 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.327, whole genome shotgun sequence.
ACCESSION   DAELLV010000327 DAELLV010000000
VERSION     DAELLV010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.327"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(78..686)
                     /locus_tag="KPD58_003510"
     CDS             complement(78..686)
                     /locus_tag="KPD58_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HBG0267234.1"
                     /translation="MEKDFFRKNGIDLAKSILGKYLIRKYENKVIVTKIIETEAYMGV
                     NDKGAHVYGNKKTDRTKPLYLDGGHIYVYLIYGMYNCLNLSANIENVPECVLIRGVEP
                     ITSLDEISMNRYNKAYTELSKYQVKNITNGPGKLCKALKIDRSLNSKSIMGEELYISD
                     FYYDDKGKKVFSKDELDIKTSKRINIDYAEEAKDFLWRFYIE"
     gene            complement(795..>2918)
                     /locus_tag="KPD58_003511"
     CDS             complement(795..>2918)
                     /locus_tag="KPD58_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HBG0267235.1"
                     /translation="GVIYLLLCNHTYYVYVYSLNNRVVYPYYELDSINKKLSKKSEQL
                     GKINLAIEKDMIIQRTLKNYIDQRKELLRQALDTIPNVWIVTDYDFNISYTNNKFKDE
                     FSKDMNNYYKILTFIKEDEKVAEKLKKFDNDISIIDKLVELNDKIYLLSLSNNKDESN
                     YLISLNDITNEIKIDEEIRNINKDYENIILNIPSPILVRSAEGGIKKNKVISINKKYE
                     KSFKCVSSDLVDMTLEQYFETFNIDFFDNREFKRLNLTQEKKAEIVSYNDTANCIVNF
                     VMSDSEGEEHVEEVRVGDYWSDNKLFKLLTFRDITKEINILRTVNEQKIIYEKLLDVI
                     PEAIFLEDLSTSRVLYTNRAFRELFGISSDVLGVTTQKYRNILVKKYINNLNIGEREK
                     SIHIVNENNHIKEVKMISRTLYFGQKRSRVRIIKDLSVQRESERLKKALIKQRQYDQM
                     KMEFYANISHELKTPLNNIYSSVQLIENLYKKGKIIDFQDILKEHIKITKQNMFRLLR
                     LIDNIINISQVKSDIYKIKAVNFDIIDITERIVTSISSYAKSKGIDLIFDTDEETVMV
                     GLDPESIERIILNILSNAIKFTLEGGEILVGIYKKDETVEIIIKDTGVGIDKEKLKDI
                     FNRFKQIENSGISNEFGSGIGLCLTKSLIEIQNGKIYIDSKVGEGTNVKVIFPIKEVV
                     EEVYDNSNYNDNIEKFEIEFFDIYK"
BASE COUNT          917 a          481 c          233 g         1287 t
ORIGIN      
        1 taaaaattct ataaaaaaat aagatacata cctataaaat accttcctat agatacatat
       61 cttacctata taaaaactta ttctatataa aaacgccata aaaaatcttt tgcttcttct
      121 gcataatcta tatttatacg tttactagtt tttatatcta actcatcttt actaaatact
      181 ttttttcctt tatcatcata ataaaaatca cttatatata attcttcacc cattatactc
      241 ttactattta gacttctatc tatttttaaa gctttgcaaa gttttcctgg tccgttagta
      301 atatttttta cttgatattt actcaactca gtgtaagctt tgttgtatct attcatagat
      361 atctcatcta aagaagttat aggctctacc cctcttatta atacacactc tggcacattt
      421 tctatatttg cagataaatt caagcaatta tacataccat aaattaaata aacatatata
      481 tgtccaccat ctaaatataa tggcttagtt ctatctgtct ttttattgcc atatacatgt
      541 gctcctttat catttacacc catgtatgct tctgtctcta tgattttggt aacaattact
      601 ttattttcat atttccttat tagatattta cctaatattg atttggctaa atcaattcca
      661 ttttttctaa aaaaatcctt ttccaaaatt tcacctcata aaatttattt aaaacgcatc
      721 aaatacaact aaagtaataa ataaaattaa ctcttattaa tgtgatatat ataaacaata
      781 aatttagaca tttattattt atatatatca aaaaattcta tctcaaactt ttcaatatta
      841 tcattgtaat ttgaattatc atatacttct tcaacaacct ctttaattgg gaatataact
      901 tttacattag ttccttctcc taccttacta tctatgtaaa tcttcccatt ttgaatttca
      961 atcaatgatt ttgtaaggca taaacctata ccacttccaa attcatttga tataccactg
     1021 ttttcaattt gtttaaatcg attaaatata tcctttaatt tttctttgtc tatgcccact
     1081 ccagtatctt ttataattat ctctactgtt tcgtcttttt tatatatacc tacaaggatt
     1141 tctcctcctt ctaaagtaaa ttttatagca tttgataata tatttagtat tattctttct
     1201 atcgattctg ggtcaagccc taccataaca gtttcttcat ctgtatcaaa aattaaatct
     1261 attcctttag acttagcata actactaata gatgtaacta ttctttctgt tatgtcaata
     1321 atatcaaaat taactgcttt aatcttatat atgtctgact taacttgaga aatatttatt
     1381 atattatcta ttaatctcaa taatctaaac atattttgtt tagttatttt tatatgttct
     1441 tttagtatat cctgaaaatc aattattttt ccttttttat aaaggttttc aataagttga
     1501 actgaactat atatattatt taaaggtgtc ttaagttcat gagatatatt agcataaaac
     1561 tccatcttca tttggtcata ctgtctttgt tttattaatg cttttttaag tctttcagac
     1621 tctctttgca cacttaaatc tttaataatt ctcactctac ttcttttctg tccaaagtat
     1681 aatgttcttg atatcatttt tacttcttta atgtgattat tttcatttac tatatggata
     1741 ctcttctctc tttctcctat atttaaattg tttatgtatt tttttactaa tatatttcta
     1801 tatttttgtg ttgtaactcc taatacatca ctagaaattc cgaacaactc tctaaaggct
     1861 ctatttgtat atagtactct tgatgtactt aaatcctcaa gaaaaatagc ctctggtata
     1921 acatccagta atttttcata tattattttt tgttcattta ctgttctaag aatatttatc
     1981 tctttagtta tatctctaaa tgtaagcaat ttaaagagtt tattatctga ccaatagtct
     2041 ccaactctaa cttcttcaac atgttcttca ccttctgagt cagacatcac aaaattaact
     2101 atacagtttg ctgtgtcatt gtaacttact atttcagctt tcttttcttg tgttagattt
     2161 aatcttttaa attccctatt gtcaaagaaa tctatgttaa aagtttcaaa atattgttct
     2221 aatgtcatat cgactaaatc acttgaaaca catttaaaac tcttttcata ttttttattt
     2281 atacttatta ccttattctt ctttatgcca ccttcagcac ttcttacaag tataggagat
     2341 ggaatattta gtataatgtt ttcatagtct ttatttatat ttcttatttc ttcatctatt
     2401 ttgatttcat ttgtaatatc atttaaactt attaagtaat tgctttcgtc tttattatta
     2461 cttaaactta acaaataaat tttatcattt aactctacca gtttatcaat tatacttata
     2521 tcgttatcaa atttcttcaa tttttcagca actttttcat cttcctttat aaaagttaat
     2581 attttataat aattattcat atcctttgaa aattcatctt taaatttatt atttgtgtaa
     2641 cttatattaa aatcatagtc tgttactatc catacatttg gtattgtatc cagagcttgt
     2701 ctaagtaatt cttttctctg gtctatgtag ttttttaatg ttctttgtat aatcatatcc
     2761 ttttctattg ctaaatttat ctttcctaat tgctctgact ttttacttaa ttttttattg
     2821 attgaatcaa gttcataata tggatacact actctattat taagactgta tacatataca
     2881 taataagtat ggttacataa tagtaaatat attactcc
//
LOCUS       DAELLV010000328         2915 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.328, whole genome shotgun sequence.
ACCESSION   DAELLV010000328 DAELLV010000000
VERSION     DAELLV010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.328"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            69..437
                     /locus_tag="KPD58_003512"
     CDS             69..437
                     /locus_tag="KPD58_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG0267236.1"
                     /translation="MENLVKIFKALSDETRLNILILVSKRNICQKGISKYLGISDSAV
                     SQHIKILKDVGIITGYKEGYYVLYHINKESFNICVDFINSMLSNSSESFIDVFDVNTI
                     HLGCSKECKSIKKCCKRREK"
     gene            440..811
                     /locus_tag="KPD58_003513"
     CDS             440..811
                     /locus_tag="KPD58_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HBG0267237.1"
                     /translation="MKVCIPVEENKGLDSKPYGHFGSAPIFVVCDLESGEVKSLDNGD
                     LDHEHGKCQPLKALSGTAVDAVVVGGIGQGAIIKLNGMGIKVYRAEGDTISANLDLLK
                     NGKLVEFPSDHTCSHDGCGHH"
     gene            complement(956..1411)
                     /locus_tag="KPD58_003514"
     CDS             complement(956..1411)
                     /locus_tag="KPD58_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0267238.1"
                     /translation="MNINTVQGDSIEVLLRQLGATRISKVSSTLYFIKFDLGDGWEIS
                     YTYNINAKDQYFLQRIEPYPIGRGLFNDEYEIVSFISKDLKKFLNAKNSSNFKTFVEV
                     TRKVNSIIDNVEELFLNYNVDGDDLKTLNKELNDILNDIDYIEKHTKKI"
     gene            complement(1450..2715)
                     /locus_tag="KPD58_003515"
     CDS             complement(1450..2715)
                     /locus_tag="KPD58_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HBG0267239.1"
                     /translation="MEFQQIIAICIFLIVMAAIITEKVNRSIAAVGGALLMIIFNILT
                     LDEGLSHIDFNTIGVLVGMMLFVAVVKNSGLFEYIAIWTAKKAKGDPWKIMICFAIIT
                     AILSAVLDNVTTVLLIGPMTIVITQILGLNPVPFLITQILASNIGGTATLIGDPPNIM
                     IGSAANLSFMDFVINLGPAVIVILAITIICFRFIYGKELVVNERAKNAILKLDEKKSV
                     KDKPLLIKSLILIAFILFGFMFHSTIHIDSSVVALTGASIMLLIGKQDVDEIMAGIEW
                     STILFFMGLFVVVGGLVEVGIINKLAQALIGLTEGHLVFTMLLILWLSAIVSSFLDNI
                     PFVATLIPLILTMQAEGIDVMPLWWATSLGACLGGNGTLIGASANVVLAGIGNKHGHP
                     ISFKEYFKIGFPLMIISIIISTVYLIIKF"
BASE COUNT         1075 a          405 c          405 g         1030 t
ORIGIN      
        1 tggttgaaaa atctaattaa gagtactata atttaattaa gtaattactt aatcaaaatg
       61 aggtacttat ggaaaacttg gttaaaatat ttaaagcatt gagtgatgaa actagactta
      121 acatacttat attagtatcc aagagaaata tatgtcaaaa ggggatttct aaatatctag
      181 ggatttcaga ttctgctgtt tcacaacata taaaaatatt aaaagatgtt gggataataa
      241 caggatataa agaaggatac tatgttttat atcatattaa taaagaatca tttaatatat
      301 gtgtagattt tataaattct atgttaagta attctagtga aagctttata gatgtatttg
      361 atgtaaatac aatacatcta ggatgcagta aagaatgtaa atctataaaa aaatgttgta
      421 agagaaggga gaaataataa tgaaagtatg tataccagta gaggagaata aaggattgga
      481 tagtaaacca tatggacatt ttggttctgc tccaattttt gttgtgtgtg atttagaaag
      541 tggagaagtt aaatctttag ataatggaga tttagatcat gaacatggaa aatgtcaacc
      601 attaaaagca ttgtcaggga cagctgtaga tgcagtagtt gttggaggaa taggacaagg
      661 agctataatt aaattaaatg gaatgggaat aaaagtttac agagcagaag gtgatactat
      721 aagtgctaat ttagacttac ttaaaaatgg aaaattagta gaatttccat cagaccatac
      781 ttgctcacat gacggatgtg gacatcatta aaaaaaaaca gctgatgaaa tatatacttc
      841 atcagctatt ttttctttta ataaatacga ttttattctt ttaaaaccaa aatttagatt
      901 tttatgtttt ttgtagtatc cttaataata tttcaagttt acaagtttac tacttttaga
      961 ttttttttgt atgtttttct atatagtcaa tatcatttaa tatgtcattg agttctttat
     1021 taagtgtttt taaatcatca ccatctacat tgtagtttaa aaatagttcc tctacattat
     1081 caatgataga attgactttt ctagtaactt caacaaatgt cttaaagtta ctactatttt
     1141 tagcatttag aaattttttt aaatcctttg atataaatga tactatttca tattcatcat
     1201 taaatagacc tctaccaata ggatatggtt caattctctg taaaaaatat tggtctttag
     1261 cattaatatt ataagtataa gatatttccc aaccgtctcc taaatcaaac ttgataaaat
     1321 ataatgtact acttactttt gatattcttg tggcaccaag ctgtcttaaa agaacttcaa
     1381 tcgaatcacc ttgaacagta ttaatattca tatatacaca tcccctttag tattattata
     1441 ctatattttt taaaatttta taattaaata aacagtagat ataataatag atataatcat
     1501 aagtggaaaa cctattttga aatactcctt aaaagagata ggatgaccgt gtttattacc
     1561 tatacctgct agtacaacat tagcagatgc tcctattagt gttccatttc ctccaagaca
     1621 agctccaaga gatgttgccc accatagtgg cattacatca ataccttcag cttgcatagt
     1681 caaaataaga ggaattagtg ttgcaacaaa tggaatatta tctaagaaag aagagactat
     1741 agctgacaac cataatatca acaacattgt aaatacaaga tgtccttctg taagtccaat
     1801 caatgcttga gccaatttgt ttattatccc aacttctact aatccaccaa ctactacaaa
     1861 taatcccata aagaataaaa ttgtagacca ttcaatacct gccataattt catctacatc
     1921 ttgttttcct attaaaagca ttatagatgc tcctgtcagt gctacaacag aagaatctat
     1981 gtggattgta ctgtgaaaca taaaaccaaa aagtatgaat gctattaata taaggctttt
     2041 tataagtaga ggtttatctt taacagattt tttttcatct agttttaata ttgcattttt
     2101 agctctttca ttcacaacaa gttcttttcc ataaataaat ctaaagcata ttatagttat
     2161 agcaagtatt actataacag caggacctaa atttattaca aaatccataa aactaagatt
     2221 ggcagcacta ccaatcataa tatttggagg gtctcctata agagtagctg ttcctcctat
     2281 atttgaagct aaaatctgtg ttattaaaaa tggaactgga ttaagtccta atatttgtgt
     2341 aataactata gtcattggtc caataagtaa gacggttgta acattatcta atacagctga
     2401 taaaatagct gttataatag caaaacaaat cattatcttc catgggtcac ctttggcttt
     2461 ctttgcagtc cagatagcta tatattcaaa taaccccgag tttttgacaa cagctacaaa
     2521 tagcatcata ccaactaata caccaatagt attgaaatct atatggctta atccttcatc
     2581 aagtgtaagt atattgaata ttatcattaa tagtgcacca ccaacagctg ctatggatct
     2641 gtttaccttt tctgtaataa tagctgccat aactattaaa aatatacata tagctattat
     2701 ttgttgaaat tccataatat aagtacctcc atttgtatta ttttgtgcct aatgctagta
     2761 ctatatatag tattactttt ttattgtttt ttcaatggat gtatgaaagt atattattta
     2821 acaaagtttt tggaaaattg acagaattta aagaaaattt ttgttattta aataaatttt
     2881 aaaattattc cattgataat aatattaaac gacaa
//
LOCUS       DAELLV010000329         2899 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.329, whole genome shotgun sequence.
ACCESSION   DAELLV010000329 DAELLV010000000
VERSION     DAELLV010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.329"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            76..1128
                     /gene="nth"
                     /locus_tag="KPD58_003516"
     CDS             76..1128
                     /gene="nth"
                     /locus_tag="KPD58_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBG0267240.1"
                     /translation="MKNVNLKEINEKDVIVEEDINLKGMDKKDVIEKEEVNKKSIDKK
                     DIIEKEEIDKKNVKKVEVQKGIDKMIGKKDEIIDEKDIENFMDIEEDKKSIKVSKKST
                     KKGETEKKTKKSSSNKATQKTSKETKSKSKIKNESANEKDVNKILDELEKLYPDAKCE
                     LNYGTAFELLIATILSAQCTDVRVNKVTSELFKKYNTARDFANLSIEEISKEIKSCGL
                     YKSKSQKIKDTSEQLCELYDGEVPDSLEKLIKLPGVGRKTAGVVLSNAFNHPAIAVDT
                     HVFRVSNRIGIVDEPNPQKTEFALMETIPKERWSHSHHVLIFHGRRMCKARNPECASC
                     PIKEDCNYYKELNETK"
     gene            1322..1792
                     /gene="trmL"
                     /locus_tag="KPD58_003517"
     CDS             1322..1792
                     /gene="trmL"
                     /locus_tag="KPD58_003517"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBG0267241.1"
                     /translation="MSLNIVLVEPEIPQNTGNIIRTCAATGSTLHLVKPLGFSLDDKH
                     LKRCGLDYWDIADIQYYDSFEELQEKYPNSKYFFSTTKAKQSHSDVKYEENCFIVFGK
                     ETKGLPEPLLKANMDTCIRVPMLDVEKARSLNLSNSVAIVVYEALRQIGYPNMR"
     gene            1828..2676
                     /locus_tag="KPD58_003518"
     CDS             1828..2676
                     /locus_tag="KPD58_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBG0267242.1"
                     /translation="MKNLKIICDSLSDVPKDLLDKYDIDIVSLTVILEEREYKDRIDI
                     SGDEFYKRLREENVYPKTSQATYAQFKEIFEKYTNEDREILYIAGSATATGTYQSAVM
                     AKNDVDGKIYTYDSNLLCMGTGLLVIKAGELASEGKSVAEILPVLDELKEKAFVIFSV
                     DTLEYLQKGGRISSTKAAIGSILNIKPILEIRDGLVSQISQVRGKKNVISKMMELIKS
                     NCGDDLSDQIVYIGYSDDFKEKEQLSKVMQDELRPKEIRFFQIGVCIGAHAGPGVTGI
                     ICLKNK"
BASE COUNT         1208 a          267 c          551 g          873 t
ORIGIN      
        1 gtttattttt tgttgatatg attaaactat ttataaatag cgttttataa attagtattt
       61 taaaggggga taattatgaa aaatgtaaat ctaaaagaga taaatgaaaa agatgtaatt
      121 gtggaagaag atataaatct aaaaggaatg gataagaaag atgtaattga gaaggaagaa
      181 gtaaataaaa aaagtataga taagaaagat ataattgaga aagaagagat agataaaaaa
      241 aatgtaaaga aggtcgaggt tcaaaaagga atagataaaa tgattggtaa aaaagatgag
      301 ataattgatg agaaagatat agagaatttt atggatattg aagaagataa aaaaagtatc
      361 aaagtaagta aaaaaagtac taaaaaaggt gaaacggaga aaaaaacaaa gaagagtagt
      421 tctaataagg ctacacaaaa aacaagtaaa gaaactaaaa gtaagtctaa aattaaaaat
      481 gagtcagcaa atgaaaaaga cgtaaataag attttagacg agttagaaaa attgtatcca
      541 gatgctaaat gtgagttaaa ttatgggaca gcctttgaac ttttaatagc aactatttta
      601 tcagcacaat gtactgatgt aagagtgaat aaagttactt cagagttgtt taaaaagtac
      661 aacacagcta gagactttgc taatttgagc attgaagaaa ttagtaaaga gataaagagt
      721 tgtggtcttt acaaaagcaa gtctcaaaaa ataaaggata catctgaaca gctatgtgaa
      781 ttatatgatg gtgaagtacc tgatagctta gaaaagctta taaaattacc tggtgttgga
      841 agaaaaacgg caggggttgt acttagtaac gcttttaatc atccagcaat agctgtagat
      901 actcatgtat ttagagttag caatagaata ggaatagtgg acgagccaaa tcctcaaaaa
      961 acagaatttg cacttatgga aactatacca aaagaaagat ggtcacactc acatcatgta
     1021 ttaatttttc atggaagaag aatgtgtaaa gctagaaatc cagaatgtgc aagttgccca
     1081 ataaaagaag attgtaatta ttataaagaa cttaatgaaa cgaaataact tttagaaagt
     1141 aagttgtaca agagggattt tctgatataa taatacttgt gaaaatgtta gataaaaaac
     1201 tattatatac atgaaataaa aattattatt aacattattt tatataatag tataatatta
     1261 ttgttattct gtttataaaa taaaatttga taaataaaag caatacttag ggaggattaa
     1321 aatgtcatta aatatagtat tagtagaacc agaaatacct caaaatacag gaaatataat
     1381 aagaacctgt gcagcaacag gttcaacttt acatttagta aaaccactgg gtttttcatt
     1441 ggatgataaa cacctaaaaa gatgtgggct tgactattgg gatatagctg atatacaata
     1501 ttacgatagc tttgaagaat tacaagaaaa gtatccaaat agtaaatact ttttttctac
     1561 aacaaaggct aaacagtcac attcagatgt gaaatatgaa gaaaattgtt ttatagtatt
     1621 tgggaaagaa acaaagggat taccagaacc tttgttgaaa gcaaatatgg atacttgtat
     1681 tagagtgcct atgttggatg ttgaaaaagc taggtcatta aatttatcaa attcagttgc
     1741 tattgtagtg tacgaggctt taagacaaat aggataccca aatatgaggt agttttaaca
     1801 agcctaaatg agaaaggagc atacttaatg aaaaatctaa agataatctg tgacagttta
     1861 tcggacgtgc caaaagattt gttagataaa tatgatatag atatagtttc attaacagtt
     1921 atactggagg aaagagaata caaggataga attgatatat ctggagatga attttataaa
     1981 aggcttagag aagaaaatgt atatccaaaa acttctcaag ctacttatgc ccaatttaag
     2041 gaaatttttg agaaatatac aaatgaggat agagagatac tttatatagc agggtctgcc
     2101 acagctacag gtacgtatca aagtgctgta atggcaaaga atgatgtaga tggtaaaata
     2161 tatacatatg actctaactt gctttgtatg ggaactggtt tacttgtaat aaaagcagga
     2221 gaacttgcaa gtgaaggaaa gtctgttgct gaaatattgc ctgttttgga tgaattaaaa
     2281 gagaaggctt ttgttatatt ttcagtagat acattggaat atttacaaaa gggaggtaga
     2341 atatcatcta ctaaggcagc aataggaagt atattaaata taaaaccgat tcttgaaata
     2401 agagatggat tagtttcgca aatatctcaa gtaagaggaa agaagaatgt aatttctaag
     2461 atgatggaat tgataaaatc aaattgtggt gatgatttat cagaccaaat tgtgtatata
     2521 ggatatagtg atgattttaa ggaaaaggaa caattatcta aggttatgca agatgaatta
     2581 agacctaaag aaattagatt tttccaaatt ggtgtgtgta taggtgcaca tgctggtcct
     2641 ggtgttactg gtatcatatg cttaaaaaat aaataatata tttttggatg acattattta
     2701 tttttttatt aaattaggat agaatattaa ttattgagat gttaaattgg atttattttg
     2761 gatgaaatta agtgttgata tattagattt agtttgtagg gaagtgtgta aatagataag
     2821 aatctattgc acactttttt atagtaagat ttattaaaaa aatttcaaaa tgtacttgta
     2881 aattgtatat aaaaaaatt
//
LOCUS       DAELLV010000330         2889 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.330, whole genome shotgun sequence.
ACCESSION   DAELLV010000330 DAELLV010000000
VERSION     DAELLV010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.330"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            100..2328
                     /locus_tag="KPD58_003519"
     CDS             100..2328
                     /locus_tag="KPD58_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HBG0267243.1"
                     /translation="MRHLKKTNSLKEILNWNNIKNLRPSKGLKNILEIFKSDLKDIRG
                     NYAVLVIVLGLAFLPSLYAWFNIKASWDPYGSTANMLVAVTNEDKGNEIAGQKINVGD
                     QLIEQLKDNKSLGWQFVDEKTALEGVKKGTYYASLKIPKNFTKDLTSLITDDVKKGEI
                     IYTVNEKINAVAPKITDKGASTIQLQVNQTIVKTVSEIVLGISNGVGAGIEHSLPKLT
                     KVENSLIDLQKRFDHINKTVDLASDATSKLSDTVKDIKSDLPTIKKTLNDTKLLSSDL
                     KKFLEDTNDNLDELSPLIKSDLNLMVDLSSSASSLTLNLIDAVNSGSEDVPKLIDNLS
                     EKLSNLQSLNDTLVDFLTKLNQLTSNNRLDDVIDNLEDSSNKIDSSISTLNDIKNKVI
                     SGQQPSISALNNVLSLSNGIGRINLNILNNFDSKISKPINSIFANSIKVANDIITVLD
                     KAEAKLPKVEEILTTSLKLSGNAQESISLIRERLPLAKGMLDDLIDTLSKISNGEDMK
                     KLVSLLESDILTKSNFLKEPVEVKTEKLYPIENYGSAMTPFYSVLSTWVGILLISALL
                     STSVHGNYKPYEIYFGRGLTFLSIALVQGFIIGTGDILLLGVTAKHPILLIVLLMFTS
                     MVFNFIVYSLVSVFGNVGKAIAIVLLVVQIGSSGGTFPIQVTPTFFQRINPIMPFTYA
                     IGATREAVGGIYAPNLTKDILVLLLFMILSICLNVLLKGPINRVIKPFNDKFGDSNLT
                     GH"
     gene            complement(2566..2865)
                     /locus_tag="KPD58_003520"
     CDS             complement(2566..2865)
                     /locus_tag="KPD58_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBG0267244.1"
                     /translation="MYKNINKKQLKEMMKNEKDVLLLDVRTKDEFKEYKIENAINISL
                     QELINNIDEIYDYKDKKVVVYCRSGHRSVTACNLLAEEGFEHLYNLNSGIIDYMS"
BASE COUNT         1082 a          389 c          394 g         1024 t
ORIGIN      
        1 attaagacaa aattttaact ttactatttg tgtaaaaagt aatataattt aaaattgatt
       61 ataaatctaa cttttttctt tatcaaaata ttttaggagg tgagacattt gaaaaaaact
      121 aatagtttaa aagaaatttt aaactggaat aatatcaaga atttaagacc ttcaaagggt
      181 ttaaaaaata tattagaaat ttttaaaagt gacctaaaag atatacgtgg caattatgcc
      241 gtacttgtaa tagttttagg tcttgctttt ttaccatctc tgtatgcttg gttcaatata
      301 aaggcatctt gggaccctta tggaagcact gccaatatgc ttgtagcagt aacaaatgaa
      361 gataaaggaa atgaaatagc agggcaaaag attaatgtag gagaccaatt aattgaacaa
      421 ctcaaagata acaagagttt aggttggcaa tttgtagatg aaaaaactgc tcttgaaggt
      481 gttaaaaaag gtacttatta tgcatcactt aagattccaa aaaattttac aaaagatttg
      541 acatctttaa ttacagatga tgttaaaaaa ggcgaaatta tctatactgt taatgaaaaa
      601 ataaatgcag ttgcaccaaa gattacagat aaaggtgctt ctacaataca actacaggtt
      661 aatcagacta tagtaaaaac agttagtgaa atcgttcttg gtatatcaaa cggagttggt
      721 gcaggaattg aacacagtct tccaaaattg acaaaagttg aaaattccct tattgacctt
      781 caaaagagat ttgaccatat aaataagact gttgatttag cttctgatgc tacttcaaaa
      841 ttatctgata cagttaaaga tattaaatct gatttaccta caattaaaaa aactctgaat
      901 gatacaaaac tattatcaag tgacttaaaa aaattcttag aggatacaaa tgataattta
      961 gacgaacttt ctcctcttat taagtctgac ttaaatttaa tggttgacct ttcatcttct
     1021 gcttcttctc ttacattaaa tttaattgat gcagtaaata gtggcagtga agatgtacct
     1081 aagttgatag ataatttatc agaaaaactt tctaatttgc aatctttaaa tgatactttg
     1141 gtggattttt taactaaatt aaaccaactt acatcaaata atagattgga tgatgtcata
     1201 gataatcttg aagactctag caataaaata gattcatcta tatcaacatt aaatgatatt
     1261 aaaaacaaag ttatatctgg acaacaacca tctataagtg ctttgaataa tgttttaagt
     1321 ctatcaaatg gtattggaag aattaattta aatattttaa ataattttga ctcaaaaata
     1381 tcaaaaccta taaatagtat atttgcaaac agtataaaag tagcaaatga tataattact
     1441 gttttagata aggcagaagc aaaacttcca aaggttgaag aaatacttac tacttctcta
     1501 aaactttcag gaaatgcaca ggaaagtata tcattgataa gagaaagact tcctttagca
     1561 aaaggaatgt tggatgattt gattgatact ttaagcaaaa ttagtaatgg tgaagatatg
     1621 aaaaaattag tcagcttgct tgaaagtgat atattgacaa agtctaattt cttaaaagaa
     1681 cctgttgagg tgaaaacaga gaaattatat ccaattgaaa attatggttc tgcaatgaca
     1741 ccattttata gtgttctttc tacatgggtt ggtatattac ttatttcagc actattgtct
     1801 acatctgtac atggaaacta taaaccttat gaaatttact ttggaagagg ccttactttt
     1861 ttaagtatag ctcttgtcca aggttttata ataggaactg gagatatatt attgttagga
     1921 gtaacagcaa aacatccaat tttattgata gttctgctta tgtttactag tatggtattt
     1981 aactttatag tgtattcttt agtttctgta tttggaaatg taggaaaagc aattgcaata
     2041 gtactattag tcgttcagat tggttcttct ggtggtacat tccctataca agttacacct
     2101 acattcttcc aaagaataaa ccctataatg ccatttacct atgcaatagg tgctacaagg
     2161 gaagcagttg gtggtatata tgctccaaat ctaactaaag atatactagt tctattacta
     2221 tttatgattc tgtctatttg tttaaacgta cttctaaaag gacctataaa tagagttata
     2281 aaaccattta atgataaatt tggcgatagt aatttaactg gtcattaaaa aattaattct
     2341 gtctttaaat atattttttt aataatattc taagttaata ttaaaaggta gtagctaaaa
     2401 agctactacc tttttccaac ttaaaaacta atatttacta caaaaactgt catagaaaaa
     2461 aacgaaaaaa ttcgttcttt cctaaattta tcttataaat atttagatat attcctactt
     2521 aataagcaga gctatacata gtatttaatg tataatttac attatttaac tcatataatc
     2581 aattattcct gagtttaaat tatataaatg ctcaaaacct tcttcagcta acaaattaca
     2641 tgcagtaaca cttctatgac cacttctgca ataaactact actttcttat ctttataatc
     2701 gtaaatttca tctatattat tgattaactc ttgtaaagat atatttatag cattttctat
     2761 tttatattcc ttaaattcat ctttagttct tacatctaat aataaaacat ccttttcatt
     2821 tttcatcatt tctttaagtt gcttcttatt tatattctta tacatatgtc taaaattcct
     2881 ccattttat
//
LOCUS       DAELLV010000331         2881 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.331, whole genome shotgun sequence.
ACCESSION   DAELLV010000331 DAELLV010000000
VERSION     DAELLV010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.331"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(65..763)
                     /locus_tag="KPD58_003521"
     CDS             complement(65..763)
                     /locus_tag="KPD58_003521"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HBG0267245.1"
                     /translation="MNEKLIGILAGMGPRSTAPFVDLIIDECQSQYGAKYDDEFPKMM
                     IYSLPTPFYIDRPINHELMKETIIDGLQKLESTGVNFIAMPCNSAHIYFKELKESINI
                     PILNIVEETVKKLPTISQKVTLFSTSSTFESTIYQNGIIHNGHEFIFKDEWQIKLNNL
                     IQNIKMDKENPHNIDIWNKLIEDVKNESIENIVIACTDLNVVLEKSYPSINIVDSSKC
                     LAESVVNKYLKLVK"
     gene            complement(925..2142)
                     /locus_tag="KPD58_003522"
     CDS             complement(925..2142)
                     /locus_tag="KPD58_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HBG0267246.1"
                     /translation="MLGKKCMDYLQTLGKISSTTNGLTRLILTQEHKKSIDLISSWME
                     GLNLDIEIDDIGNVIGTYKSSFPNAPTLVVASHQDSVKCGGIFDGMLGIIVPLVGLEE
                     AKHNNRSYPFNIKLIAFAEEEGTRFETSLMGSKVFAGTFKEELLKSVDENGITLEEAV
                     TKFGFNTKNLTNLHPRKDVDAYLEFHIEQGPVLENESLPAGIVSSITGFKSFKISVNG
                     KSGHAGTLPMNMRLDAGCCACECVLAIEKVAKTTTDLVATVGKMNFYPSSSNVVPERA
                     EFTLDVRSCSQEILDNSVEKIFNEISHICENRKLNYTSELAFENVPVPCSNKITKIIE
                     KSFIDLNLNPFYIYSGAGHDAQEMDNITDIGMVFIRCAGGVSHNPNESVSVDDLDTAV
                     KIFLKILDNLDLK"
     gene            complement(2333..2746)
                     /locus_tag="KPD58_003523"
     CDS             complement(2333..2746)
                     /locus_tag="KPD58_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0267247.1"
                     /translation="MELEITEKIEKQDEDTIFQGLLKYNLARLEDKNPVDLGIYIRDK
                     AGKIVAGFIGVTHGNWLSIKYLWVSEKLRYKGTGSQLLYKAEKIAKERGCKYVFLDTF
                     SFQAPKFYEKFGYHKVFSLENYPLTGKRYYYRKIL"
BASE COUNT         1003 a          435 c          322 g         1121 t
ORIGIN      
        1 taagcctata ctatttaaga taaacttata acatatattt tttataatat agtgcatgaa
       61 aaatttattt aactaatttt agatatttat tgacaactga ttcagccaag cattttgatg
      121 aatcaacaat atttattgaa ggatatgatt tttctaaaac tacatttaaa tcagtacaag
      181 caattactat attttctata gattcatttt taacatcttc gataagttta ttccaaatat
      241 caatattgtg tggattttct ttatccattt ttatattttg aataagattg tttaatttta
      301 tttgccattc atctttaaat ataaattcat gtccattatg tattatacca ttctggtaaa
      361 ttgtagactc aaaagttgaa ctagtcgaaa aaagagttac tttttgtgag atagtaggta
      421 attttttaac tgtttcttct acaatattta atattggtat attaatagac tcttttaatt
      481 ctttaaagta tatatgtgct gaattgcatg gcattgcaat aaaatttaca ccagttgatt
      541 caagcttttg taatccatca attatagttt ctttcataag ttcatgattt attggacggt
      601 caatataaaa tggggtaggt aatgaatata tcatcatctt tggaaattca tcatcatatt
      661 ttgctccata ttgagattga cactcatcaa taattaaatc aacaaaaggg gctgttgacc
      721 ttggacccat tccagccaaa ataccaatta atttttcatt cataaaaatt atctccttta
      781 tatataaata tatttatgta taaaataaaa atctataaaa gcataaaatt atattttata
      841 atagattata ttgtacactt atataatata aatataaagt gattttttag attatatatt
      901 ttaatatact ttcttatata agaattattt taaatcaaga ttatctagta tttttaaaaa
      961 tatttttaca gcagtatcca agtcatctac agaaacactt tcattaggat tatgacttac
     1021 gcccccagcg catcttataa ataccatacc aatatcagtt atattatcca tttcctgagc
     1081 atcatggcca gcaccactat aaatataaaa aggattaaga tttaaatcta taaaactttt
     1141 ttctatgatt tttgttattt tattagaaca tggtacagga acattttcaa aagcaagttc
     1201 acttgtataa ttaagttttc tattttcaca tatatgtgat atttcattaa atatcttttc
     1261 tacagaatta tcaagaattt cttgtgaaca agagcgtaca tcaagagtaa attcagctct
     1321 ttcaggtacc acatttgatg aagatggata gaaattcatc tttcctacag tagcaaccaa
     1381 atcagtagtc gttttagcta ctttttcgat agctaataca cattcacagg cacaacatcc
     1441 agcatcaagt ctcatattca ttggaagtgt accagcatgt ccagacttac catttactga
     1501 tatcttaaaa cttttaaatc ctgtaatact tgatacaatt ccagcaggaa gagattcatt
     1561 ttctaaaaca ggaccctgct cgatatgaaa ttctaaatat gcatctacat cttttctagg
     1621 atgcaaattt gttaagtttt ttgtattaaa tccaaacttt gttacagctt cttcaagtgt
     1681 aattccattt tcatcaacag atttaagaag ttcctcttta aaagtaccag caaaaacttt
     1741 acttcccatt aaagaagttt caaatctagt tccttcctct tcagcgaaag caattagctt
     1801 aatattgaaa ggataagacc tattattgtg ttttgcttct tcaagaccaa ctagaggcac
     1861 tattatacct aacataccat caaatatacc tccacacttt acactatctt gatgagatgc
     1921 aactactaat gtgggagcat ttggaaagct tgatttatat gtacctataa cgtttccaat
     1981 gtcatcaatt tcaatatcaa gatttaaacc ttccatccat gaactaatta aatcaataga
     2041 ttttttatgt tcttgagtca agataagtct tgtaagacca tttgttgttg aacttatttt
     2101 tcctaatgtt tgtagataat ccatacattt ttttcctaac atatttacct cctaaaaata
     2161 taatttttat ttaagctaag attagctttt atacttataa tttttgaact ttcaaataat
     2221 tttgtatgta tagtaaaata ctattttagt attaataatg atataaaaac ataaagtctg
     2281 tagacgaaga gcagaagttt taatttaaaa atatgaaata tgatttttat tattatagta
     2341 tttttctata ataatatctc tttccagtta gaggataatt ttcaagtgaa aatactttat
     2401 ggtatccaaa tttctcataa aatttaggtg cttgaaaact gaatgtatct aaaaaaacat
     2461 atttacaacc tcgttcttta gcaatttttt cagccttata taaaagttga cttccagtac
     2521 ctttatatct aagcttctca ctaacccata agtattttat agaaagccaa ttaccatgag
     2581 taacacctat aaaacctgca acaattttac ctgctttatc tcgaatatat attcctaaat
     2641 ccactgggtt tttatcttca agtctagcaa gattatattt taataatcct tgaaaaatag
     2701 tatcttcatc ttgtttttct attttttctg taatttctaa ttccatattt gaactttctc
     2761 cttgtataat cttttgtata ttggtatttt ttaataaaat cagtagaaag aatatagttt
     2821 aacattattc taataggcat ttatgctaat gtcaaactaa taatacttat tcttaatttt
     2881 a
//
LOCUS       DAELLV010000332         2824 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.333, whole genome shotgun sequence.
ACCESSION   DAELLV010000332 DAELLV010000000
VERSION     DAELLV010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.333"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            271..2055
                     /locus_tag="KPD58_003524"
                     /pseudo
     CDS             271..2055
                     /locus_tag="KPD58_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
BASE COUNT         1246 a          401 c          501 g          676 t
ORIGIN      
        1 ttatacaaaa ccctattttt tataacaatt cattaacata aaaatcaaac ttaccttaaa
       61 atataaattt acaaattttt caatcaataa gaaaatttct ttattcctta cataatgact
      121 gcaatgtaac aaaataatta caaattaatt atgaaatcat tatagttaga aacctcttta
      181 aaattagata ttaagtatcc tttagaatta gaacgggaac aaagttaaaa aattaagtaa
      241 tatcaaatgg acaaggggga agaaaataaa atgaaaatac caaagaagat tgctgcaatg
      301 cttacagtta caatgatagc tggaagttca acagcaggca tagctagtgc acaaactgtg
      361 gcaacaaatt taacagggca agaaagatat gaaactgctg ttaaaataag tcaagatgga
      421 tggaaaaatg ctgatgaggt agtaatcgta aacgactcat ctatagcaga tgctctatct
      481 gcaacaccat ttgcaaaagc aaaaaatgct ccaatacttt taacaagtaa agataaatta
      541 aatgataaga cgaaagcaga aatacaaaga ttaaaagcta aaaaagtata cttaataggt
      601 ggaacttctg tattaagtac aaatatagaa aaagaaataa aaaatttaaa aatatctttt
      661 gagagaatct ctggagctga gagatatcaa acatccttag aattagcaaa gaaattagat
      721 gctataagtg atgttaagaa aatagctgta gttaatggtg aaaaaggact tgcagatgct
      781 gtaagtgtag gagcaccagc tgcacaaaac aatatgccag taatacttgc tgattctaaa
      841 aatggaacag cagttgcaga caaatttata aaagatgctg gaataactca atcttatgta
      901 gtaggtggag agagttcaat ttctgaagct gttaaaaata aactaccaaa tagtacaaga
      961 ctaggtggaa cagatagaaa tgatacaaat gcaaaagtaa taaaagaatt ttataaaaag
     1021 acagatttaa aaaatgctta tgtaactaaa gatggtatga ataaacaaga ccaacttata
     1081 gatgcactag cagtaggtgt attaggtgct aaaaatcaat caccagtagt tttagttgga
     1141 aagaacttat cagcttcaca aaaatctcta gttaactcta agagttttga taagataact
     1201 aaagttggtg ggaatggtaa tgagacagca tttaatgaaa tgaagtcatt acaagaagta
     1261 aaaacagttg aagctaaaac aatttctgaa ttaaaatcag ctatagataa agctactgct
     1321 aatgatgtta taaactttaa gccaactagt gaggttaaag aagcatttac aatacaaact
     1381 gataaagcag taacagtaaa cttaaatggt acatatacaa agactgtaac tatcaacatg
     1441 cctaatggag atgtaaataa ctatgcaaaa gtggatgatt tagttataga tgatgtaaaa
     1501 gatggaactt ttgttaacta tggaaaaata actaacttaa aggttaatga taaaaatgga
     1561 gccaagatag aaaataattc taaaggtgag attggtagtt taacagtagc atctggagca
     1621 agtcaagtaa aagtgactaa tggtggtaag ataactacag ttacaaataa ttctaaaggg
     1681 actactatag ataataaagg tacaataagt agtgttaagg gagataattc tccaacaata
     1741 agtggaaata gtccaagttc taattcatca ggaggaagtt catcaagtgg aggttcttca
     1801 catggaggag gctcatcttc aggaggaagt tcttctaatc aaacatcagt aaataatgaa
     1861 gcagcaaaaa taacaagcgt gcctacacca gcaaaagatg caactagatt aactatgcca
     1921 tcagtatcaa gtggatatac tatagcaata aaaacatcaa gtaatgagag tgttataaaa
     1981 aaagatggaa caataatacc tccaaataca gcaacaacag taaaattagt atttacggta
     2041 acacatacat caagtggtaa aacagcagat acaaaagaac tatctgtaac agtaccagct
     2101 aaatcaacag atgaagaatt acaagcagca ttagacaatg aagtggcaaa aataacaagc
     2161 gttcctgcac cagcaaaaga tgcaactaaa ttaactatgc catcagtatc aagtggatat
     2221 actatagcaa taaaaacatc aagcaataag agtgttataa aggaagatgg aacaataata
     2281 cctccaaata cagaggaaac agtaacattg gtatttacag taacacaaga atcaagtggt
     2341 aaaacagcag atacaggaga aatagatgta gtagtaccag ccaaatcaac agatggggaa
     2401 atacaagcag aagtacaagc tgaggcggat aaaataacaa gtgtaactca gccaacacaa
     2461 gatgcaacaa cactaactat gccaacagta gcaagtggat acacaataaa gataaaatca
     2521 tcaacaaatg aaagtgttat agcgaaagat ggaacaatag tgccaccaga tacagaaaca
     2581 aaagtaacat tagtatttac agtgacacac acatcaagtg gtaaaacagc agatacagga
     2641 gaaatagaag taacagtacc agccagaaca gctatagata cagaacaatt ggcagtacaa
     2701 gctgaggcag ataaaataac aagtgtaact cagccaacac aagatgcaac aacactaact
     2761 atgccaacag tagcaagtgg atacacaata aagataaaat catcaacaaa tgaaagtgtt
     2821 atag
//
LOCUS       DAELLV010000333         2738 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.334, whole genome shotgun sequence.
ACCESSION   DAELLV010000333 DAELLV010000000
VERSION     DAELLV010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.334"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            16..762
                     /locus_tag="KPD58_003525"
     CDS             16..762
                     /locus_tag="KPD58_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0267248.1"
                     /translation="MKILTVNNLSKVYGKKIIFNALNDINFSIEDGEFVGIMGPSGSG
                     KTTLLNMISTIDKPTTGTMELKGKNPLLLRGEELALFRRRELGFVFQDFNLLDTLTIG
                     ENIVLPLTLDKVSVKEQDERLNEVSTILGIKDLLGKRTFEVSGGQAQRTAIARALINN
                     PSILLADEPTGNLDSKSSKVVMELFQKINKENKVTTMMVTHDPLAASYCSRILFIKDG
                     SIYNEIYKGSSREQFYQEIMDVLTLLGGDN"
     gene            762..2678
                     /locus_tag="KPD58_003526"
     CDS             762..2678
                     /locus_tag="KPD58_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0267249.1"
                     /translation="MNIKQFAINNISRNIKAYLGYLASIVISSSLLFSFIMFTSHPDL
                     DVSQFPDYLKEGLKMSKIIAYLFLFFFVFYSVSVFLKSRYKEFGILYITGISKRQVMK
                     LIFIENMIINIVSSVIGIIIGLIFAKIFLVVMSTFLELSPLKFYIPFNAILSTLIYFM
                     ILAVLTSIFTSFIVKENQVLKLLKGTKTPKPEPKTSLMLAILSICLFIIAYYNAITST
                     DQYTTYLRLVPVTSLTIVATYLFFSQFSVFFMKKLKLNKRFYRKNTNMLWISNLIYKV
                     KDNARIFFLITITSAVAFTAIGTVYSFWKDVERQINLIYPNTIYYSTMTLHNDPKKPD
                     SKEKDKERMMFIENNLKKENVDYTRTNGEFKTVFPKKSDFNVRIMKESKYLEITETIG
                     VKPISFEDNECISLLSSSLPGNKNIKENIIVGNRGLKVAKQVEECVMPAYYKNMYVVK
                     DNFYDSIESKFIVDRFTAFEVEDSSKTIEICRKFENKFNNESGMQPYLFFSKELMIGT
                     GKLMYSTFIFLAIFIGLIFFVTTSSFLYNKFYMDCQVDKKKYEQLNKLGMTYNEIKKA
                     STIEIGIVFLFPYVVAVIHSIFALTALKNSFDIEVASSAVLVMGSLFLVQIVYFLIVR
                     NNYLKEIRLNLVNF"
BASE COUNT         1041 a          288 c          450 g          959 t
ORIGIN      
        1 aaggatagtt aaataatgaa aattttaact gtaaataatt taagtaaagt atatggtaaa
       61 aaaataattt tcaacgcatt aaatgacata aattttagta tagaagatgg agaatttgta
      121 ggaattatgg gtccatctgg tagtggaaaa actactcttt taaatatgat ttctactatt
      181 gacaagccaa ctacaggaac tatggaatta aagggcaaga atcctttatt gttaagaggt
      241 gaggaacttg cattatttag aagaagagag ttaggttttg tatttcagga ttttaattta
      301 ttggatacat taactatagg agaaaatata gtattgccat tgacattaga taaggtcagt
      361 gtaaaagagc aagatgagag attgaatgaa gtatctacaa tattaggtat aaaagattta
      421 ttaggaaaaa gaacttttga agtatcaggt ggtcaagctc agaggactgc tattgctaga
      481 gcattgatta acaatccatc aatactttta gcagatgaac caacaggtaa tttagattca
      541 aagtcatcaa aggttgtaat ggagctattt caaaagataa ataaagaaaa taaagttact
      601 actatgatgg taactcatga cccattagca gcgagctact gtagtagaat cttattcata
      661 aaagatgggt ctatatataa tgaaatatat aagggtagta gtagagagca gttttatcaa
      721 gagataatgg atgtacttac attattggga ggcgataact aatgaatata aaacagtttg
      781 ctattaacaa tatttcaaga aatattaaag cgtatttagg atacttagca agtattgtta
      841 tatcatcttc actgctattt tcttttatta tgtttacaag tcatccagat ttagatgtat
      901 ctcaatttcc tgattattta aaagaaggtc tcaagatgag taagataatt gcatatctat
      961 tcttattctt ctttgtgttt tattcagtta gtgtgtttct taaaagtaga tataaagaat
     1021 ttggaatact ttatattaca ggtatatcaa aacgccaagt aatgaaatta atctttatag
     1081 aaaatatgat aataaatata gtatcttctg taattggaat aataataggc ttaatctttg
     1141 caaagatatt tttagtggtt atgtctactt ttttagaatt atcaccatta aaattctata
     1201 taccatttaa tgcgatattg tctactttaa tctattttat gattttagct gtattaacat
     1261 caatatttac ttcttttata gttaaagaaa atcaggtgtt gaagttactt aaaggtacaa
     1321 aaactccaaa accagagcct aaaacatcac taatgcttgc tatattaagt atttgtttgt
     1381 ttataatagc gtattataat gctattacct caaccgacca atatacaaca tatctgcgct
     1441 tagttccagt tacatccctt actatagtag caacttactt gttcttttct caatttagtg
     1501 tattttttat gaagaagcta aaattaaaca agagatttta tagaaaaaat acaaatatgc
     1561 tttggatttc gaacttgatt tataaggtaa aggataatgc tagaatattt ttcttgataa
     1621 ctataacttc tgcagtagca tttactgcta taggtacagt ttattccttt tggaaagatg
     1681 tagagcgtca aattaattta atatatccta acacaatata ttattcaaca atgacattgc
     1741 ataatgaccc taaaaagcct gatagcaaag aaaaagacaa ggaaagaatg atgtttatag
     1801 aaaataatct aaaaaaagag aatgtagact atacaaggac taatggtgaa tttaaaactg
     1861 tatttcccaa gaaaagtgat tttaatgtca gaattatgaa agaatctaag tacttagaaa
     1921 ttacagaaac tataggggtt aagcctataa gttttgaaga taatgaatgt atatcattat
     1981 tatcatcaag tttaccagga aataagaaca tcaaagaaaa tattattgta gggaatagag
     2041 gtttaaaggt ggcaaaacaa gtggaagaat gtgttatgcc agcatattac aaaaatatgt
     2101 atgtggtaaa ggataatttt tatgatagta tagaatcaaa atttatagtg gatagattta
     2161 ctgcatttga agtagaggat tcaagtaaaa ctatagagat atgcagaaaa tttgaaaata
     2221 aatttaataa tgagtcaggg atgcaaccat atttgttttt ctctaaagaa ttaatgatag
     2281 gaactgggaa attaatgtat tctacattta tatttttagc aatatttata ggtttaattt
     2341 tctttgttac tacaagtagt tttttatata ataaatttta tatggattgc caagttgata
     2401 agaaaaagta tgaacagtta aataaattgg gtatgacata taatgagatt aaaaaagctt
     2461 caacaattga gattggaata gtatttttgt ttccttatgt agtggctgtt atacattcaa
     2521 tatttgcact tactgcactt aaaaattctt ttgatataga agtagcatca tcagctgttt
     2581 tggtaatggg aagcttgttc ttggttcaaa tagtttattt tttaattgta agaaataatt
     2641 atttaaaaga gataaggtta aatttagtca atttttagtt actattatta aatgataata
     2701 aaaagggttt agaattaatt aatattactg gaattata
//
LOCUS       DAELLV010000334         2652 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.335, whole genome shotgun sequence.
ACCESSION   DAELLV010000334 DAELLV010000000
VERSION     DAELLV010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.335"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            61..882
                     /locus_tag="KPD58_003527"
     CDS             61..882
                     /locus_tag="KPD58_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG0267250.1"
                     /translation="MGYKLIVTDMDGTLLGNNHKVTDENKTALQKVIKSGINVTLATG
                     RAFDSAKCNVDFLKEDMPIIACNGSLIREQNGNIIYSNKIDTRTCLNILDVLDKYDIY
                     YQCNSIDSMLSKKIEGREDRLSVFLGSETEVIVKDDLREEIFKKDILKFVIIEEKNPS
                     ILDEIRKELRKVQGIKITSSWPNNIEVMNEGVDKGNAVKILAEKMNIDREDIIAFGDN
                     YNDIEMIKFAGLGVAMGNAEELIKQEADYVTDTNQDSGVAKAIYKFLELKESLSS"
     misc_binding    961..1172
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1259..2575
                     /gene="argH"
                     /locus_tag="KPD58_003528"
     CDS             1259..2575
                     /gene="argH"
                     /locus_tag="KPD58_003528"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HBG0267251.1"
                     /translation="MKLWGGRFRKAENQLMEEFNKSFGYDCVLYKKDIEGSVAHVHMQ
                     VKCGLLTEEEGKSITEGLKGILEDVENGKLVLDGEYEDIHSFTEINLIQRIGDVGKKL
                     HTARSRNDQVAVDMRLYAKEKANDLVGLISEFKATIKDVADKNPVMMPGYTHLQRAQV
                     VTFKHHLMAYYSMFDRDEKRIKNAIEILDESPLGCGALAGTTHDIDRSITCEQLGFKK
                     VVDNFMDGVSDRDYLLELMSDFSIIMMHLSRLSEELILWSSQEFGFVEIDDLYTTGSS
                     IMPQKKNPDGAELIRGKTGRVYGNLFGLFTVMKGIPLAYNKDMQEDKEGFFDSVHTLE
                     MCIQIMDRMIATLKVNEDKMKQAVKNGFLNATEVADYLVKNNVAFRDAHGIVGSIVIY
                     CEDNKKAIEDLTLEELQKFSDAFKEDIYDFIDYESILNKGIKKNLK"
BASE COUNT         1076 a          242 c          532 g          802 t
ORIGIN      
        1 aattctttag attatattaa ggaaatatag gtatacaata aaaagttatg gagttggatt
       61 atgggatata aattaattgt aacagatatg gatggaactt tattaggcaa taatcataaa
      121 gttacagatg aaaataaaac tgcactacaa aaagttataa aaagcggtat taatgtaact
      181 ttagcaacag gaagagcctt cgattcagct aaatgcaatg tagattttct taaagaggac
      241 atgccaataa tagcttgtaa tggttcatta ataagagagc aaaatggaaa cataatatat
      301 tcaaataaga ttgatacaag aacatgttta aacattttag atgtattaga taaatacgat
      361 atttactacc aatgtaatag tatagattct atgctttcga aaaaaataga aggacgtgaa
      421 gatagattaa gtgtattttt aggctctgaa acagaagtaa ttgtaaaaga tgatttgaga
      481 gaagaaatct ttaaaaaaga tatactaaaa tttgtaatca tagaagaaaa aaatccatct
      541 attttggatg aaataagaaa agaattgaga aaagttcaag gaataaagat aacttcttca
      601 tggccaaaca atattgaagt catgaatgaa ggtgttgata aaggtaatgc agttaaaatt
      661 ttagctgaaa aaatgaatat agacagagaa gatataattg catttggaga caattacaat
      721 gacatagaaa tgataaagtt tgctggtctt ggggtagcta tgggaaatgc agaagaattg
      781 attaaacaag aagctgatta tgttacagat acaaatcaag acagtggagt tgcaaaggca
      841 atttataaat tcctagagtt aaaagaatca cttagctctt aaaacagtat ataataggaa
      901 aaaataggtt gactattttg tagatagtaa ttataataca aatagataaa acataaaaat
      961 ttagattcag tgaaaaagag agtagatata gagaaaatct cagcgaattg ggggatggtg
     1021 tgagcctaat gataacactt tatattgaaa agaactttgg agaatactac ttgaaattca
     1081 gtatggtagt aggtaatctc gccttaagag aatagagtgg gtaaacattg tttaccaact
     1141 agagtggtaa cacggaatat ttcgtctctt ggtctttttg accaagagat tttttagttt
     1201 cattgagtta caagacttat aattaaaata caaaaaatta aaatatgagg tgaaagttat
     1261 gaaattatgg ggcggacgct ttagaaaagc ggaaaatcaa cttatggaag aatttaataa
     1321 atcttttggc tatgattgtg tactgtacaa aaaagatatc gaaggaagtg tagcacatgt
     1381 tcatatgcaa gtaaaatgtg gacttttaac agaagaagaa ggaaagtcta taacagaagg
     1441 tttaaaaggg atacttgaag atgtagaaaa tggaaagtta gtattggatg gagagtatga
     1501 agatatacat agttttactg agataaattt aattcaaaga attggagatg ttggcaagaa
     1561 gcttcataca gcacgtagta gaaatgacca agttgcagtt gatatgagat tgtatgctaa
     1621 agaaaaagca aatgatttgg tgggtttaat atctgaattt aaagctacta taaaagatgt
     1681 tgctgacaaa aacccagtga tgatgccggg ctatactcat cttcaaaggg cacaagtagt
     1741 gacatttaag catcacttaa tggcatatta tagtatgttt gatagagatg agaaaagaat
     1801 aaaaaatgct atagaaatat tagatgaatc acctttaggc tgtggagctt tagcaggaac
     1861 tacgcatgat atagatagaa gtataacttg tgaacaatta ggatttaaaa aagtagtaga
     1921 taattttatg gatggagtaa gtgatagaga ttatttacta gagctaatga gtgatttttc
     1981 aattataatg atgcacttaa gtagattaag tgaagagttg atactttgga gttctcaaga
     2041 atttggattt gttgagatag atgatttata tacaactgga agtagtataa tgcctcagaa
     2101 gaaaaatcca gatggtgcag agcttataag aggtaagact ggtagagtat atggaaactt
     2161 atttggtctt tttacagtaa tgaaaggaat accattagca tataataaag acatgcaaga
     2221 agataaagaa ggttttttcg atagtgtaca tactcttgaa atgtgtatac aaataatgga
     2281 tagaatgata gccacactaa aggtaaatga ggataaaatg aaacaagcag taaaaaatgg
     2341 ttttttaaat gcaacagaag ttgcggatta tttggttaaa aataatgttg cttttagaga
     2401 tgcacatggt atagtaggaa gtatcgttat atattgtgaa gataataaaa aagcaataga
     2461 agatttaaca ttagaagaat tacaaaagtt ttcagatgct tttaaggaag atatttatga
     2521 ttttatcgac tatgagagta tattaaataa gggaataaaa aagaatttaa aataatgtat
     2581 ttatttttaa aagctgtaaa tttgaatgtt atcatttaaa tttacagctt tttattgtgt
     2641 aagattttat ta
//
LOCUS       DAELLV010000335         2649 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.336, whole genome shotgun sequence.
ACCESSION   DAELLV010000335 DAELLV010000000
VERSION     DAELLV010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.336"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..805
                     /locus_tag="KPD58_003529"
     CDS             <1..805
                     /locus_tag="KPD58_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG0267252.1"
                     /translation="NIVSLLLIFEYNLRVNTITTENLFIFIFAILLILSSSIILLIVI
                     GKIVQDDKLNLEYELINERIKTNYRNYENINEVHDRLKYVYHDLKNHMICIKNYDTKE
                     EIISYINNLEFQISEFEHFKNTGNKTLDLILAEKISICKKYDIQIEDNINISKLNFIK
                     NNDICSIFANSLDNAIEACMDINNEIEKRIEVKATYINKFAIIKFINTKVNDIKLIDK
                     RIQTSKDDDNIHGIGLASIKYIVNKYGGETIVNYSDNEFILKIMIPIKS"
     gene            817..957
                     /locus_tag="KPD58_003530"
     CDS             817..957
                     /locus_tag="KPD58_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HBG0267253.1"
                     /translation="MKKIALNLLKNISALSFGIAVLSANSASSWVAHQAKEPQALQKL
                     KK"
     gene            1016..1597
                     /locus_tag="KPD58_003531"
     CDS             1016..1597
                     /locus_tag="KPD58_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBG0267254.1"
                     /translation="MFKRLSYKFANILVNNEIVESEDFEIYRYGFETLIYFIVNILVA
                     LFIGIIFDRFIHTVIFLSCYCTLRQFTGGYHARNYKECTLTFVVIYLITIFSANNIDI
                     NKYKYLLVLLMIISILTIYKLAPLEHRNKPLSESEKKHYRKTVQKILFVIICLIILCK
                     ILNIFQQYVIYAIISIYWIAILIYIGMKVNSDQ"
     gene            1854..2228
                     /locus_tag="KPD58_003532"
     CDS             1854..2228
                     /locus_tag="KPD58_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0267255.1"
                     /translation="MNLKKKIKKLGVTFAIGTIIVSSLGTSVFADEVSTYALISKAFS
                     GKNNIISSSVYTSGSRTFKTTDSQGGFSCRLTERFSGSSPDRIVKRHEANASSKVSLS
                     GGTYYIRYEGSTNNAKTYATLN"
     gene            2311..>2649
                     /locus_tag="KPD58_003533"
     CDS             2311..>2649
                     /locus_tag="KPD58_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0267256.1"
                     /translation="MSSIKVISDFITKIIRKNILFAITIIIINYHLIQRWTHISNRGI
                     SFNDYLSLSVYTCMTISILTMLNVIFYMRKSYDTVKILCRNNFDYFLGISVAVTRNNF
                     IFCCIPIIYAI"
BASE COUNT         1100 a          242 c          380 g          927 t
ORIGIN      
        1 taatatagtt agcttattac ttatatttga atataattta agagtcaata caattactac
       61 agaaaactta tttatattta tatttgcaat tttattgatt ttatcatcaa gtataatact
      121 tttaatagta atagggaaaa ttgtacaaga cgataaatta aatctagagt atgaacttat
      181 aaatgaaaga ataaaaacaa actacaggaa ttatgaaaat ataaacgaag ttcatgatag
      241 attaaaatat gtttatcatg acttgaaaaa ccatatgatt tgtataaaaa attatgacac
      301 taaagaagaa attatatcat atataaataa cttagagttt cagataagtg aatttgaaca
      361 ttttaaaaat actggaaata aaacattaga tttaatttta gcagaaaaaa taagtatttg
      421 taaaaagtat gatattcaaa ttgaagataa cataaatata tctaaattaa actttattaa
      481 aaataatgat atatgttcaa tatttgcaaa ttcactagat aatgcaattg aagcatgtat
      541 ggatataaat aatgagatag aaaaaagaat agaagttaaa gctacatata taaataaatt
      601 tgcaataata aaatttataa atactaaggt aaatgatatt aaacttattg ataaacgtat
      661 tcaaactagt aaggatgatg ataatataca tggaatagga cttgctagta ttaaatatat
      721 agtaaataaa tatggtggtg aaacaatagt taattactca gataatgagt ttatattaaa
      781 aataatgata ccaataaaaa gttaggagag tacaaaatga aaaaaatagc gttaaactta
      841 cttaaaaaca tctcagcatt aagttttggg atagctgtat tatcagcaaa cagtgcaagt
      901 tcttgggtag cacatcaggc aaaagagcca caagctttac aaaaactaaa aaaataacat
      961 taataaaagg acctcttctt attaaaggag aggttttaca tgttgaggag gaaatatgtt
     1021 taaaagatta tcatataaat ttgccaatat attagttaat aatgagattg tagaaagtga
     1081 agattttgaa atatatagat atggatttga aactttgatt tatttcatag ttaatatttt
     1141 ggtagcatta tttataggaa ttatttttga tagatttata catacagtta tatttttaag
     1201 ttgttattgc actttaagac agttcacagg aggttatcat gctaggaatt ataaagagtg
     1261 tacattaaca tttgtagtta tttatttgat aactattttt tcagctaata atatagatat
     1321 aaataaatat aagtatttat tagtattatt aatgattatt agtatactaa caatttacaa
     1381 gttagctcct ttagaacata gaaacaaacc tcttagtgaa agtgagaaaa agcattatag
     1441 aaagactgtg caaaaaatac tattcgtaat aatatgttta attattttat gtaaaatact
     1501 taatattttt cagcaatatg tgatttatgc aataatttct atatattgga ttgctatatt
     1561 aatatatatt ggaatgaagg ttaatagtga tcagtaagat tataaaacat tttctaaatt
     1621 aattaacttc actaattaat attaacttta aaaaaataat gtcaaggtaa gtatttgaac
     1681 ttattaaata tagttgaatc tattgtgggt tgtatcaaag gacaaataga ttcacttaat
     1741 aaccagtttg taacagagta ttgaattgta aaagttaata cgatagtatg aaaatgtaat
     1801 ttgaaattac atttttataa aaattatatt aactaagggg agggatacat acaatgaatt
     1861 tgaagaaaaa aattaaaaag ttaggagtta catttgcaat aggaactata attgtatcaa
     1921 gtttagggac atctgtattt gcagatgagg ttagcacata tgctttaata tctaaagctt
     1981 tttcaggtaa aaacaatatt atatctagtt cagtatacac ttctggaagt agaaccttta
     2041 aaacgacaga ttctcaaggg ggattttctt gtaggctaac agaaagattc tcaggatcta
     2101 gcccagatag aattgtaaaa agacatgaag caaatgcttc ttcaaaagtt agtttatcag
     2161 gaggaacata ctatataagg tatgaaggtt caacgaataa tgctaaaaca tatgcaactt
     2221 taaattagta ttaaaagcat cctataatta tttttatagg atgtttttgt aattgttata
     2281 atcaaaagac tggctaggag gggcataaat atgagtagta ttaaggttat atcagacttt
     2341 ataaccaaaa taattagaaa aaatatttta tttgcaataa caattattat tataaattac
     2401 catctcatac aacgatggac acatatatcc aatagaggaa tttcatttaa tgattactta
     2461 tccttgagtg tatatacttg tatgacaatt agtattttaa ccatgttaaa tgtaattttt
     2521 tatatgagaa aaagttatga tactgtaaaa atattatgta gaaataattt tgattatttt
     2581 ttaggtattt ctgttgctgt aactagaaac aactttatat tttgttgcat accaattata
     2641 tatgctata
//
LOCUS       DAELLV010000336         2621 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.337, whole genome shotgun sequence.
ACCESSION   DAELLV010000336 DAELLV010000000
VERSION     DAELLV010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.337"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            625..894
                     /locus_tag="KPD58_003534"
     CDS             625..894
                     /locus_tag="KPD58_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBG0267257.1"
                     /translation="MFESRCGVCCDSCTKKEQVNCTGCPTMEKPFWGGECKVKTCCES
                     KELNHCGECDTFPCDMLLNKGKDQGFDPMVNIEQCRKWLEETVSN"
     gene            complement(1112..1357)
                     /locus_tag="KPD58_003535"
     CDS             complement(1112..1357)
                     /locus_tag="KPD58_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pathogenicity locus"
                     /protein_id="HBG0267258.1"
                     /translation="MGELQKIPGVGKATEKSLIMLGYTTIKLLKDANPAQMYEKECLM
                     RGQHIDRCQLYVYRCAVYFASTENPEPEKLKWWYWKD"
     gene            1783..2481
                     /gene="tcdC"
                     /locus_tag="KPD58_003536"
     CDS             1783..2481
                     /gene="tcdC"
                     /locus_tag="KPD58_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin anti-sigma factor TcdC"
                     /protein_id="HBG0267259.1"
                     /translation="MFSKKNEGNEFSNEGKGSSKKIIKFFKSTKGIALLAFILGVFFG
                     NISSPACSEDHEEVISNQTSVIDSQKTEIETLNSKLSDAEPWFKMKDDEKKAIEAENQ
                     RKAEEAKKAEEAKKAEEQRKKEEEEKKGYDTGITYDQLARTPDDYKYKKVKFEGKVIQ
                     VIEDGDEVQIRLAVSGNYDKVVLCSYKKSITPSRVLEDDYITIRGISAGTITYESTMG
                     GNITIPGIAVEKIN"
BASE COUNT          958 a          285 c          394 g          984 t
ORIGIN      
        1 aaatcattca atatattaaa taaatattta actttaataa tctatattta attatagcta
       61 taacatagaa ttatcaaata tattgaatga atatttgact cttggaatgt gtaattaaat
      121 taagtttggt tattcgatgg aaggcaaatt tgatataaaa ctccatttaa agtgccagat
      181 aaaaacttta tggttatata aaagttaaaa gtaatttata gtgttataga atattcaaag
      241 ggatttaaag taatctatgg tgttttgaaa tattcaaagg gatttaaact aatctatagt
      301 cttgtagaat attcaaagag atttaaaaca atctataatg ttgtaaaata tattcaaagg
      361 gatttaaact aatctatagt cttgtagaat attcaaagag atttaaagca atctataatg
      421 ctgtaaaata tattcaaagg gatttgaagt aatttattgt gttttgaaat attcaaaaag
      481 atttttggaa tagtttttat aaatttacca gtactaataa ttttatgttt aacatttaga
      541 tgacctaaaa tcaactattt gcacttttgt attcatctaa aatcatttta taatattttt
      601 aaataatgga aaggatgttt gaacatgttt gaatcaagat gtggtgtctg ttgtgacagt
      661 tgtacaaaaa aagaacaagt aaattgcact ggttgcccaa ctatggaaaa accattttgg
      721 ggtggagaat gtaaggttaa aacttgctgt gaaagtaagg aacttaatca ttgtggtgaa
      781 tgtgatactt tcccttgtga tatgctttta aataagggaa aagaccaagg ttttgaccct
      841 atggtaaata ttgagcaatg tagaaaatgg cttgaagaaa cagtttctaa ttaattgttt
      901 tagaatgctt tgttaagtaa tttatttaat cctttgttag gtagtatgtt tagtcatttg
      961 ttgattggta tatttataca tttattgatg ggtatattta gccatacaaa tagatttaca
     1021 taaaatttac gactaaaaaa gagttgagta ttagttttta ttatttaaaa cggatattca
     1081 actctttttt atgtctaact tttttgattt tttaatcttt ccaataccac cactttaatt
     1141 tttctggttc tggattttca gttgatgcaa aatatactgc acatctgtat acatataact
     1201 gacatctgtc tatgtgctgt ccacgcatta aacattcctt ttcatacatt tgtgctgggt
     1261 ttgcatcttt aagtaattta atagttgtat atcctaacat gattaaagat ttttctgttg
     1321 ctttccctac ccctggaatt ttttgtagtt ctcccatact tcaccttctt tctggtatat
     1381 tctttttata ttatacctat taccagatat ttttttatta tagttaatat ttaatattta
     1441 attttttatt atatcaattt atttatgctc tttcatatat atatatttta ccacctctaa
     1501 atcactgagt cacttaatta catcataata tagttttata caaataaaat actttatgtt
     1561 taatttaata tataaaattc accttcaaga aaattatatt ataatctgac atttttatct
     1621 tattttttca aaatatattg aatcttctta ctttattttg taaaattatg cttaggggaa
     1681 atatatttta ggaaaatatg aatatataat ttttagtcaa ctagttattt taagttttta
     1741 acttttaaaa taaaatatat ctaataaaag ggagattgta ttatgttttc taaaaaaaat
     1801 gagggtaacg aatttagtaa tgaaggaaaa ggaagctcta agaaaataat taaattcttt
     1861 aagagcacaa agggtattgc tctactggca tttattttag gcgtgttttt tggcaatata
     1921 tcctcaccag cttgttctga agaccatgag gaggtcattt ctaaccaaac atcagttata
     1981 gattctcaaa aaacagaaat agaaacttta aatagcaaat tgtctgatgc tgaaccatgg
     2041 ttcaaaatga aagacgacga aaagaaagct attgaagctg aaaatcaacg taaagctgaa
     2101 gaagctaaaa aagctgaaga agctaaaaag gctgaagaac aacgcaaaaa agaagaagag
     2161 gagaagaaag gatatgatac tggtattact tatgaccaat tagctagaac acctgatgat
     2221 tataagtaca aaaaggtaaa atttgaaggt aaggttattc aagttattga agatggtgat
     2281 gaggtgcaaa taagattagc tgtgtctgga aattatgata aggttgtact atgtagttat
     2341 aaaaaatcaa taactccttc aagagtatta gaggatgatt acataactat aagaggtata
     2401 agtgctggaa ctataactta tgaatcaact atgggtggaa atataactat accagggata
     2461 gctgtagaga aaattaatta atattgtttt gtattatagt taatatttta tattatagtc
     2521 aatatgttta aagatgtttt tataattgca aataaacagt tacaaggctc taaattagtt
     2581 tttgctttta gcatattatc tattttttat caactattaa t
//
LOCUS       DAELLV010000337         2581 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.338, whole genome shotgun sequence.
ACCESSION   DAELLV010000337 DAELLV010000000
VERSION     DAELLV010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.338"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            454..933
                     /locus_tag="KPD58_003537"
     CDS             454..933
                     /locus_tag="KPD58_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG0267260.1"
                     /translation="MDKTTFTNNILESEQTLYRVSKSILGNDQDCEDAVNNAILKAYE
                     KLDSLKEEQYFKTWLIRIVINECNSLRRKRLKSLSFEDVFKNKKIDEKDDYSDLYTAI
                     QSLPKKIKIPIVLYYIEGYSVDEVKEILDIPQGTVKSRLSRGRRLLKTKLENTEVII"
     gene            930..1709
                     /locus_tag="KPD58_003538"
     CDS             930..1709
                     /locus_tag="KPD58_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0267261.1"
                     /translation="MNKNILNNSFPEVPESFHNSLSKTLNSLPEREENYKMINNKTHK
                     LPFRKGLVATLAITLILSTTALAVGHIYSLVGKSSTKATYTSIPTTEQVKKDFNFTPN
                     IVSEFSNGYKFKGAYTVNKKAFDKENNVLGKSKALSFEYKNGNDEISLDTANNVLGKS
                     SDNLKVVDTYKDINISYEAFAQKFVPEGYKMTEQDKIDEKSGKYTFSVGPNNESDKVE
                     VNKFKFLTWKQDEVYYSFTVQDSNLSMNDLVKMAHEVIDAK"
     gene            complement(1959..2459)
                     /locus_tag="KPD58_003539"
     CDS             complement(1959..2459)
                     /locus_tag="KPD58_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0267262.1"
                     /translation="MIEGIKFIKAEEKYIYSYWQTFDKIAKERKYLAMDEAFPFEETV
                     EFIKNIINKNLPQLFIIDLESDNCIGWCDVLPKTEKVGYLGMGILKEYREKGIGSNLL
                     KQIIDLSKEYGYEKIELDVFKSNSRAIHVYKSLGFVEVNTISSGFTWNDRPVKEEVIQ
                     MELTLI"
BASE COUNT         1013 a          349 c          317 g          902 t
ORIGIN      
        1 taaaataatg acatttctaa ctcttaattc tcatttaagt ttcattgttt tttcttaaaa
       61 gcctactttt agtcaagata ctgttatctt tactcattat ttaaagaact tattttaaat
      121 ctgtacatga agtaaataat ccaaaagcat tgttattcta actactcaac attaaatact
      181 tactattttc taattactca atataaacac ttactataaa actaaatata taaatggctt
      241 ctaaagttta atatatctac atggctttat atatgatggg aaaatactag aaaaaaatat
      301 attatagaat atttgtatag aataggcaaa actttcaaac ttaaatttat caaataatca
      361 taaaaaaagt gaaccatcta tagtaaaatt ttatctaata agtgaaaggc aaaaaagtag
      421 cgctacgatt tgcatagaag ggaaggaact tagttggata aaactacatt tacaaacaat
      481 atacttgaat cagaacagac tttgtatcga gtatctaagt ctattctagg aaacgaccaa
      541 gactgtgaag atgccgtcaa caatgctatt ttaaaagcat atgaaaagtt ggattctctt
      601 aaggaagaac aatactttaa aacatggctg attagaatag tcataaatga atgtaattca
      661 ttaagacgca aacgattaaa atctctatca tttgaagatg tcttcaaaaa caaaaaaatt
      721 gatgaaaaag atgactatag cgatttgtat actgctattc aaagtctacc taaaaaaatt
      781 aaaattccaa tagtccttta ctatattgaa ggttattcag tagatgaagt taaggaaata
      841 ttagatatac ctcaaggaac tgtaaaaagt agattatcaa gaggaagaag attattaaaa
      901 actaaattag aaaacacgga ggtaatcata tgaataaaaa tatattaaat aattcgtttc
      961 cagaagttcc agaaagtttt cataatagtt taagcaaaac actaaatagt ttaccagaaa
     1021 gagaggaaaa ttataaaatg attaataata aaacacacaa attaccattt agaaaaggct
     1081 tagttgcaac tttagccatt actttgatat tgagcacaac agcccttgca gtaggacata
     1141 tatatagttt ggttggaaaa tcaagtacta aggctaccta tacatctatc ccaactacag
     1201 aacaagtaaa aaaagacttt aactttaccc ctaatatagt ttctgaattt agtaatggat
     1261 ataaatttaa aggagcatat actgtaaata aaaaagcatt tgacaaagaa aataatgttt
     1321 taggaaaatc aaaagcactt agttttgaat ataaaaatgg caatgatgaa atatctcttg
     1381 acacagcaaa taatgtactt gggaagagtt ctgacaatct aaaagttgta gatacttaca
     1441 aagatattaa tatttcttat gaagcattcg cacaaaagtt tgtaccagaa ggttataaaa
     1501 tgacagaaca ggataaaatt gatgaaaaat caggcaaata cacatttagt gtagggccaa
     1561 ataatgaatc agacaaagtt gaagttaata aatttaagtt tttgacatgg aaacaagatg
     1621 aagtttacta ttcttttaca gttcaagact caaatttatc tatgaatgac ttagttaaaa
     1681 tggctcatga agtaatagat gcaaaataaa cataagtaat aataagttgt ttatgagttt
     1741 attatttatg actttaattt ttttattagt aaacatggtt gtatcttcca tctttatatt
     1801 ttctttaaat ttaatatata aaattttaga tatttagcta ccttcaaaag aaccattatt
     1861 aaaaagccct taaaaatatt tcttttatat ggaattttaa gggctttaac tttattttat
     1921 acaagtgtat atggaatttt aaagacttta actttatttt atataagtgt aagctccatt
     1981 tgaattactt cttcttttac aggtctatca ttccatgtaa aaccactaga tattgtattt
     2041 acctctacaa accctaaaga tttataaaca tgaatagctc tactattact tttaaataca
     2101 tcaagctcaa ttttttcata cccatattct tttgataaat caataatttg ttttaaaaga
     2161 ttacttccga tacctttttc cctatactct ttaagaatac ccatgcctaa ataaccaact
     2221 ttttcagttt taggtaaaac gtcacaccaa ccaatacagt tatctgattc taaatcaatt
     2281 ataaacagtt gtggtaagtt tttatttata atatttttta taaattcaac tgtttcttca
     2341 aaaggaaagg cttcatccat agctagatat ttcctctctt ttgctatttt atcaaaagtt
     2401 tgccaatacg aataaatata cttttcttct gcctttataa attttatgcc ttctatcaac
     2461 ttaacatttc ccctttttaa tttgtgattg tagttaactt attttatatg attctagttt
     2521 ataatgctat ataaattact atatattagc ttaaaatatc tttgtatatt tctataaaat
     2581 t
//
LOCUS       DAELLV010000338         2565 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.339, whole genome shotgun sequence.
ACCESSION   DAELLV010000338 DAELLV010000000
VERSION     DAELLV010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.339"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..237
                     /locus_tag="KPD58_003540"
     CDS             <1..237
                     /locus_tag="KPD58_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBG0267263.1"
                     /translation="KNKIKDEDVEKILRDTNYISNYNVDKYDEGRSVILESIVNTKSL
                     GDKIICLNLLQTLNGVKEMYRISNITKVSIKEAA"
     gene            451..1308
                     /locus_tag="KPD58_003541"
     CDS             451..1308
                     /locus_tag="KPD58_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0267264.1"
                     /translation="MSKDVKVKRNKKYDTFSITMELERPLLYDEVENKDDKNNNRKFN
                     IKNKIFKGIGILFVLILLGFFIWINKTYEPQKLAKEALVSNSKVEVTVNENISFTPKG
                     RKVSKGLILYPGAKVEIESYAPLARKIAESGYEVVIVKMPLNLAILGTNKAQKVMDSY
                     NNIEHWVIGGHSLGGVAASNFARDNKLIEGVVFLASYPMGDELKELGKKVISIWGSKD
                     GVVNFKSLVESKQKLPDDTTYVEIEGGNHAQFGDYGKQKGDNDAIISQEKQLNITANS
                     IIKFLKNIS"
     gene            1405..2274
                     /locus_tag="KPD58_003542"
     CDS             1405..2274
                     /locus_tag="KPD58_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG0267265.1"
                     /translation="MEYDMPLYRPPSEAYSLIIQVTLGCSHNKCTFCSMYKSKKFKIK
                     PLEVIKNEIDIFRRYYKHVERIFLADGDALIIPMEKLRDIILYIKEVFPECERITMYG
                     SPKSIEKKSDDELKELRKLGVKMIYLGLESGSNEVLEDIKKGFSSEQLIKVGRKVKKS
                     GIKLSATVIAGLGGTKKTHQHAVDTGKMLGAISPDYVGVLSLMVEPNTELYDLLKAGE
                     FTILEDKAVLQEIKEMIKNIDANEKIVFRSNHASNYANLKGVLPDDKQRLIDEIDYYL
                     SNQKLKREEYRRL"
BASE COUNT         1093 a          233 c          430 g          809 t
ORIGIN      
        1 aaaaataaaa taaaagatga agatgttgaa aaaatattga gagataccaa ttatatttca
       61 aattacaatg tagataagta tgatgaagga agaagtgtaa ttttagagag tattgtaaat
      121 actaaaagtc taggtgacaa gataatatgt ctaaacttac tgcaaactct aaatggtgtc
      181 aaagaaatgt atagaataag taatataact aaagtttcaa taaaagaagc agcttagata
      241 ttaaattata gaatagccat atatttaaaa taaagtatat ggctatttgt tttataaaat
      301 actaaaagaa aataatatat aaaaaacaaa aacatatatt tatgaatttt atatgaaaat
      361 agttaaaata ttgatttttt tcattggttg aaaaaacaat ttacttagga taaactataa
      421 tagtattcaa atattatggg ggttagattt atgagtaaag atgtaaaggt aaagcgtaat
      481 aagaaatatg atacatttag tataactatg gaacttgaga gacctttact ttatgatgag
      541 gttgagaaca aagatgataa aaataataat agaaaattta acataaagaa taagatattt
      601 aagggaatag gtatactatt tgtattgatt ttgcttggct ttttcatatg gataaataaa
      661 acatatgaac ctcaaaagct ggcaaaagaa gctctagtca gcaatagtaa agttgaagta
      721 actgtaaatg aaaacatttc atttacacca aaaggaagaa aagtttctaa aggtttaatt
      781 ttgtatccag gagctaaagt agaaatagaa tcttatgctc cacttgcaag aaaaatagca
      841 gaaagtggat atgaggttgt aatcgtaaag atgccactta atctagctat acttggaact
      901 aataaggcac agaaagttat ggattcttac aacaacatag aacattgggt aataggaggt
      961 cattcattag gaggagttgc agcatcaaat tttgcacgtg ataataaatt aattgaaggc
     1021 gttgtatttt tagcttctta tccaatgggt gatgagttga aagaattagg aaaaaaagtt
     1081 atatctatat gggggagcaa agatggagta gtcaatttta aaagtcttgt agaatcaaaa
     1141 caaaaacttc ctgatgatac aacttatgtt gagattgaag gaggaaatca tgcacaattt
     1201 ggagattatg gaaaacaaaa gggagataat gatgctatta taagtcaaga aaaacaactt
     1261 aatattactg ccaatagtat cattaaattt ttaaagaata tatcttaaac taaaatttaa
     1321 aaaatataat tttataagtt attatttaaa gtaaatatta ataatataat agaatgaata
     1381 ttttattaca agggggattt taatatggaa tacgatatgc cactttacag accaccaagt
     1441 gaggcttata gtcttataat acaagtcaca ctaggatgtt ctcataacaa atgtacgttt
     1501 tgtagtatgt acaaatctaa aaaatttaaa ataaagccac tagaagtaat aaaaaatgaa
     1561 attgatatat ttagaagata ttataagcat gtagaaagaa tattcttagc agatggagat
     1621 gcattgataa taccaatgga gaaattaaga gatattattt tatatataaa agaagttttt
     1681 ccagagtgtg aaagaattac tatgtacggc tctcctaaat ctatagagaa aaaatctgat
     1741 gatgaactta aagaattaag aaaacttggt gttaaaatga tttatctagg gcttgaaagt
     1801 ggaagcaatg aagtattaga agatattaag aaaggtttta gtagtgaaca attaataaaa
     1861 gttggtagaa aagttaaaaa aagtgggatt aaattatcag ccactgtaat agcaggtctt
     1921 ggaggaacta aaaaaacaca tcagcatgca gttgatacag gaaaaatgtt aggagcaatt
     1981 tcaccagatt atgtgggtgt acttagttta atggtagaac ctaatactga actttacgat
     2041 ttacttaagg caggagagtt tacaatacta gaggataagg ctgtgttaca agaaattaaa
     2101 gagatgataa agaatataga tgctaatgaa aaaattgtat ttagaagtaa tcatgcatca
     2161 aattatgcta atttaaaagg tgtattacct gatgataaac aacgtctaat tgatgaaatt
     2221 gattactatc taagtaacca aaagctaaaa agagaagaat atagaagact atagtttttg
     2281 atatatcaat ctatattgat aggtaaatac taagattatt tagatacaaa aaatcatgct
     2341 acattaatag ttgcttacta ttgatgtagc atgatttttt tattaaaaaa caaaatggta
     2401 tattcatata taatatatca ataataatga aaatgtgaac tagtttaata gaattttacc
     2461 tattataata aaaacaggaa tttaaataat aaaaagtaaa gtaacttttt attaagtaac
     2521 tttacttttt attttattta atgttgtatt aaatttatat atatt
//
LOCUS       DAELLV010000339         2562 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.340, whole genome shotgun sequence.
ACCESSION   DAELLV010000339 DAELLV010000000
VERSION     DAELLV010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.340"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            6..806
                     /locus_tag="KPD58_003543"
     CDS             6..806
                     /locus_tag="KPD58_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBG0267266.1"
                     /translation="MTFKNKKFTINKYKILSIILVLCLILTAFSSYVNYSHKKFKDYV
                     VFYVQHQDDEVLWAGSAIVNAIKERGKNHVFVVLVSTGCGISVFDKYKKYENLTNIQK
                     SEYRNREFLASLNSLGVIRENIILLPETNTNGNTDFNYMEKIALTFEEKFKNVTHIAH
                     TYKLDDHLQHLKNGSVIQNLYNAGKIKDAKYFVKPKFADKITFNNKIVYKAHSTEDYE
                     KVKNACQQYKIVDELEYREGIGYKSDHKSFDKLLNNKDVPAILHTPNL"
     gene            1346..2062
                     /locus_tag="KPD58_003544"
     CDS             1346..2062
                     /locus_tag="KPD58_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0267267.1"
                     /translation="MNQNILVVDDDREIVDAIEFYLRPDNFNVLKAYDGLEALDILIE
                     NDIKLIIMDVMMPNLDGLKTTLKIREKRNIPIILLSAKSEDMDKIIGLNMGADDYITK
                     PFNPLELTARVKSQLRRYINLGDFSNTNNSNDTNYILKSGGLELNTDTKIVSLDGEEI
                     KMTPLEYKILTLLMSRKGKIFSSHEIYENVWNEDAYSCERTIAVHIRRIREKIEINPK
                     EPKYLKVVWGIGYKIEKLNY"
BASE COUNT         1006 a          285 c          338 g          933 t
ORIGIN      
        1 attaaatgac atttaagaat aaaaaattta ctataaataa atataaaatt ctatctatta
       61 ttctagtttt atgtttgatt ttaaccgctt ttagtagcta tgtgaattat agtcataaaa
      121 aatttaaaga ttatgttgtc ttctatgttc agcatcaaga tgatgaagtg ttgtgggctg
      181 ggagtgctat tgttaatgct ataaaagaaa gaggaaaaaa tcatgtattt gtggttttag
      241 tatctactgg atgtggtata tccgtctttg ataaatataa aaaatatgaa aacctaacaa
      301 atatacaaaa atcagaatat agaaatagag aatttttagc ttctcttaat agccttggtg
      361 taataaggga aaatataata ttattaccag aaacaaatac aaatggtaat actgacttca
      421 attacatgga aaaaattgct cttacatttg aagaaaaatt taaaaatgta actcatatag
      481 cacatactta taagttagat gaccatttac aacatttaaa aaatggttct gttatccaaa
      541 atttatataa tgctggtaaa ataaaagatg ctaaatattt tgtaaaacct aaatttgctg
      601 ataaaataac ttttaataat aagatagttt ataaagctca ttctactgaa gactatgaaa
      661 aggttaaaaa tgcctgtcaa caatacaaaa ttgttgatga attagaatat agagaaggta
      721 ttggctataa gtctgaccat aaatcatttg ataagttatt aaataataaa gatgttcctg
      781 ctatacttca tacacctaat ttataagaaa tatttattct tttaatttaa gttataaaaa
      841 ggaaattatt tgagattttg tgatatagca ctttttgtgc tatactactt tttctcatac
      901 aactattacc catatctaat ttcatcatct cttaaaatat ataaagatat catcattaaa
      961 acctataagc cttattaaat gctttataca tacctacatt aaactacctg tttatattaa
     1021 actacattaa aataaatatt tgttataaat tcttaataat tatgaaactc atttgttatt
     1081 tatatttgtt aatatatgtt taaacctaaa aaattaataa accaaaataa cataagaagt
     1141 gtgatagatt accctagata ttttcataaa cttttacctt cagattaata tgggcgtcta
     1201 tacttttaaa tttactatat gcattgacaa tatattaaaa gtactagttt ttatttcata
     1261 atgaacgggc ttactgtttt ctaatacctc tttttggtat aaaataaaaa ttggttaaaa
     1321 ataaaatttc aagggggtta ataagttgaa tcaaaatata ttggttgtag atgatgatag
     1381 agagatagtt gatgctatag agttctattt gagacctgat aactttaatg ttttaaaggc
     1441 atatgatgga cttgaagcat tggacatact aatcgaaaat gatattaagt taataataat
     1501 ggatgtaatg atgcccaatt tagatgggct taaaactaca cttaaaataa gagaaaaaag
     1561 gaatatacca ataattctct tatctgcaaa atcagaagat atggacaaaa ttataggtct
     1621 taatatgggt gctgatgatt atataactaa accatttaat ccactagaac ttactgctcg
     1681 agtaaaatct caacttagaa ggtatataaa tttaggtgat tttagtaata caaataactc
     1741 aaatgatact aattatattc taaaaagtgg tggcctagag ctaaatacag atacaaaaat
     1801 agtttcttta gatggtgagg aaattaaaat gacaccactt gaatataaaa tacttacact
     1861 tcttatgtca cgaaaaggta aaattttctc ttctcatgag atttatgaaa atgtctggaa
     1921 tgaggatgct tatagttgtg agagaactat tgctgttcat attagaagaa ttagagaaaa
     1981 gattgaaatt aatccaaaag aaccaaaata tttaaaggtg gtgtggggca ttggttacaa
     2041 aattgaaaaa cttaattatt aatgattcta aagcaaaatt tattttatta ttattactgt
     2101 ttctattagt ttctatatta tttgaagcag tttatgattt gaataataac ttttcttata
     2161 atattaattg ttattataaa gagtttgctc tcccttattt ttattttttc ggtactcctg
     2221 tacatttgtc aaaagtagaa ttagagtttg tctgcatagg tattatatta tcaagttttt
     2281 tattgactat actatgtttt acattgtata aaaaaaataa acagactaaa gtcaaattct
     2341 tagagaatgt tataagcctt ttttctaagg taaaaataga acttacactt atattaactt
     2401 tgatactatt aaccttaagg tttcatgcat ttacaagtaa taaaatgttt tacaacacta
     2461 atttaataat ctacaatttt ataattatat ctatagtcta tataatatat gaaaatatta
     2521 aatataataa ctataatcta aaagatatgt ccataacata tg
//
LOCUS       DAELLV010000340         2557 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.341, whole genome shotgun sequence.
ACCESSION   DAELLV010000340 DAELLV010000000
VERSION     DAELLV010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.341"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            61..2403
                     /gene="secA"
                     /locus_tag="KPD58_003545"
     CDS             61..2403
                     /gene="secA"
                     /locus_tag="KPD58_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBG0267268.1"
                     /translation="MSVLDVILDKTDELEIKKLNNIVDKIDALEDKIQILDDEALKNM
                     TNVFKDKLSKGETLDDILPEAFAVVREVSKRILGMRQYRVQLIGGIVLHQGKIAEMRT
                     GEGKTLVGVAPVYLNALTGKGVHVITVNDYLAQRDKELMQPVYEFLGLTVGVIINGQQ
                     PVERKLQYQCDITYGTNNEYGFDYLRDNMVTQKSEKVQRELNFAIVDEVDSILIDEAR
                     TPLIIAGGGDEGTKLYELANAFIKNIRDEDFEMDRKDKTIALTASGLSKAEKFFGIEN
                     ITHIDNIEIFHHVNQSLRAHKLMNIDVEYVVRDNEILIVDEFTGRIMDGRRFTDGLHQ
                     AIEAKEGVKINDESKTMATVTFQNFFRLYSKLSGMTGTAKTEESEFEATYKINVVQIP
                     TNKPVIRADLHDKVFKTERAKYNAVVEEIIRINKTKQPVLVGTVSIEKSEKLSKLLKK
                     KGVKHEVLNAKQHDKEAEIISKAGKLGSVTIATNMAGRGTDISLGAGDKEEEEKVKEL
                     GGLYVIGTERHETRRIDNQLRGRSGRQGDPGTSRFFVSLEDEVIKLYGGKSIEKILKR
                     MNANEDDALESKALNRAIERAQKGIEGKNFEQRKNVLKYDDTINEQRKVIYDERNKVL
                     DGSDIEEEIQKMVMDIITEAAEKYLKKFRDYHGYFKYLYNTFMPADTLLIPDLDKKNV
                     EEIVNQTYQISKRVYDLKKMMIGIEELAQLEKRVLLQVVDTYWVDHIDAMDQLRQYIG
                     LKSYAQKDPFKEYAIEGYEMFEALNRNIRITTVQYLYKFN"
BASE COUNT         1054 a          259 c          520 g          724 t
ORIGIN      
        1 ctattctata attttaaggt ataatagtaa gtggaaaata aataaggagg taaattaaat
       61 atgtcagttt tagatgtaat attagacaaa acagatgaat tagaaattaa aaaattaaat
      121 aatatagtag ataaaataga tgctttagaa gataaaatac aaatactaga tgatgaagca
      181 ttaaaaaata tgacgaatgt atttaaggat aagttaagta aaggagaaac cttagatgac
      241 atattgccag aagcatttgc tgttgtaaga gaagtatcaa aaagaatatt aggtatgcgt
      301 caatatagag ttcaattaat tggtgggatt gtattacatc aagggaaaat agcagagatg
      361 agaactggag aaggaaagac tctagtagga gtagctccag tttacttgaa tgcattgact
      421 ggtaaggggg tacatgtcat cacggttaat gattacttgg ctcaacgtga taaagaactt
      481 atgcaaccag tatatgaatt tttaggttta acagtaggag taattattaa tgggcaacag
      541 ccagtagaac gaaaattaca atatcaatgt gatattactt atggtacaaa caatgagtat
      601 ggattcgatt atttaagaga taatatggta acgcaaaaat cagagaaagt tcaaagagaa
      661 ctaaactttg ctatagttga tgaggttgat tcaatactta tagatgaagc tagaacacca
      721 cttataattg ctggtggagg agatgaaggt actaagctgt atgaacttgc aaatgctttt
      781 ataaagaata taagagatga agattttgaa atggatagaa aagataaaac catagcactt
      841 actgcaagtg gtcttagtaa agctgagaaa ttttttggaa tagaaaatat aactcatata
      901 gataatatag aaatttttca ccatgtaaat caatctctaa gagcacataa attaatgaac
      961 atagatgttg aatatgttgt tagagataat gagatactaa ttgtagatga atttacagga
     1021 agaataatgg atggaagaag gtttactgat gggcttcatc aagcaattga agctaaagag
     1081 ggagtaaaaa taaatgatga atcaaagacc atggcaacag taacatttca aaatttcttt
     1141 agattatact ctaaattatc aggaatgaca ggtacagcaa agacagagga aagtgagttt
     1201 gaagctacat ataagattaa tgttgttcag ataccaacta ataaaccagt tataagagct
     1261 gatttacatg ataaagtatt caaaacagaa cgtgcaaagt acaatgctgt tgtagaagaa
     1321 ataataagga taaataaaac taagcaacca gtgcttgtag gaactgtttc aattgaaaaa
     1381 tctgagaaat tatctaagct acttaaaaag aaaggtgtaa agcacgaggt cttaaatgct
     1441 aaacaacatg ataaggaagc tgaaataata tctaaagctg gtaaattagg ttctgttaca
     1501 attgctacta atatggcagg tagaggaact gatatatcac ttggagctgg agataaagaa
     1561 gaagaagaaa aggtgaaaga gttaggtggc ttgtatgtta taggtactga aagacatgag
     1621 acaagaagaa ttgataatca gcttagaggg cgttcgggaa gacaaggtga ccctggaact
     1681 tctagattct ttgtaagttt agaggatgaa gtaataaaat tgtatggtgg aaaaagtata
     1741 gaaaaaatat taaagagaat gaatgctaat gaagatgacg cgttagaaag taaggctttg
     1801 aatagagcaa tagaaagagc acagaaaggc attgaaggta aaaattttga acaaagaaag
     1861 aatgttctta agtacgatga tactattaat gagcaaagaa aagtaatata tgatgaaaga
     1921 aataaggtat tggatggttc tgatattgaa gaagagatac aaaaaatggt tatggatatt
     1981 attacggagg ctgctgaaaa gtacttaaaa aaatttagag actatcatgg atattttaag
     2041 tatttatata atacttttat gccagctgat actttattaa ttccagactt agataaaaaa
     2101 aatgttgaag aaatagtaaa tcaaacatat cagatttcaa agcgtgttta tgatcttaag
     2161 aagatgatga ttggaattga agaattggct caattagaga aacgagtatt acttcaagtt
     2221 gtggatactt attgggttga ccatatagat gcaatggatc aactaagaca atatataggt
     2281 cttaaatctt atgcacaaaa agatccattt aaagagtatg ctatagaagg ttatgaaatg
     2341 tttgaagctt taaataggaa tataagaata acaacagtgc aatatctata taaatttaac
     2401 taaaaaagtg acccattggg tcacttttta tatattaaga ttaaagtgaa atttatttta
     2461 cagtacacac tcatatagat aaatatttat aataatgaaa aagaggctga acgtcagcct
     2521 cttttcctgt gtgagaaata cggaaaaaag agatgct
//
LOCUS       DAELLV010000341         2556 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.342, whole genome shotgun sequence.
ACCESSION   DAELLV010000341 DAELLV010000000
VERSION     DAELLV010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.342"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1257
                     /locus_tag="KPD58_003546"
     CDS             <1..1257
                     /locus_tag="KPD58_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBG0267269.1"
                     /translation="IFSIHEITLNTENFKRARKESVNLIKGKIIRTIIYSLILSLVVA
                     IIGYAIYHSGIIFIGIWTKYCTDMEFLKNVFINRCIAFEEYFRLILSIFIFILDIGFI
                     SATYYQYKGVSISKNNIKKEKKSISKIVFNLVLILLISYIESVAFSSHINGMFNTSFS
                     FYTTAIAHRAGAEMAPENTLAAIEEASHAKADYAEIDVQETKDGELIVLHDSNFKRTT
                     GVDKNVWDVNYNEVKTYDAGSFYYYGKGYYDEKFVGEKIPTLKEKIKYSKGKVKLLIE
                     IKLNGHEKGDVVKKVVQLIKENNFENQCIVASMDKTILQKVKKLDENLLTCYFMAIAY
                     GDFSKLNYVDIYGIESTFVNKTVVEKIHKMNKKIFVWTVNSDSLIEKMLDLNVDSIIT
                     DNPYLIESAIYWKKNGFISQVSDYLF"
     gene            1579..2163
                     /locus_tag="KPD58_003547"
     CDS             1579..2163
                     /locus_tag="KPD58_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBG0267270.1"
                     /translation="MTEREKMLAGELYDCGDIELLTQWHKAKDLVRDYNQTDSSDLEK
                     KEKILNELLGGKGKNLWITAPYYVDYGNNIYFGNNCEVNMNCTFLDDNKIIIGDNALI
                     APNVQIYTAFHPTNAGDRFGNAKEDGSFEFCKTQTAPVTIGDNVWIGGGAIIMPGVTI
                     GDNVVIGAGSIVTKDIPSNMIAYGNPCRIIRENK"
BASE COUNT         1003 a          241 c          406 g          906 t
ORIGIN      
        1 atttttagca ttcatgagat aactttaaat acagaaaatt ttaaaagagc aagaaaagaa
       61 agtgttaatc ttataaaggg aaagattata agaacaatta tttattcatt aatattatct
      121 ttagtagtag ctattattgg atatgcgatt tatcatagtg gaattatatt tataggcata
      181 tggactaaat attgtactga tatggaattt ttgaagaatg tatttataaa tagatgtatt
      241 gcatttgaag aatattttag attgattttg tcaatattca tttttatatt ggacattgga
      301 tttatctcag caacttatta tcaatataaa ggagtaagta tttctaaaaa taatattaaa
      361 aaagagaaaa aatctatatc aaaaattgtt tttaatttag tattaatatt attaatttct
      421 tatatagagt cagttgcatt ttcttcacat ataaatggta tgtttaatac atcattttca
      481 ttttatacaa cagcaattgc tcatagagca ggagctgaaa tggctccaga gaatactttg
      541 gcagcaatag aagaagcttc acatgctaaa gctgattatg cagaaataga tgttcaagaa
      601 actaaagatg gagaacttat tgtacttcat gattcaaact ttaagagaac tacaggagta
      661 gataaaaatg tttgggatgt aaattataat gaagtaaaga catatgatgc gggaagcttt
      721 tattattatg gaaaaggtta ttatgatgaa aagtttgttg gggaaaagat accaacactc
      781 aaagaaaaga taaaatattc taaaggtaag gtgaaattat taatagaaat aaaacttaat
      841 ggacatgaaa agggagacgt agtaaaaaaa gttgttcagt taattaaaga aaataatttt
      901 gaaaatcaat gtatagttgc atctatggat aaaactatat tacaaaaagt aaaaaaactg
      961 gatgagaatt tactgacatg ctattttatg gcaattgcat atggtgattt ttcaaagtta
     1021 aattatgttg atatatatgg aattgaatca acttttgtta ataaaacagt agtagaaaag
     1081 attcataaaa tgaacaaaaa aatcttcgtt tggactgtta atagtgacag tttaattgag
     1141 aaaatgcttg acctaaatgt agatagtatt attacagata atccatacct tatagagagt
     1201 gcaatttatt ggaagaaaaa tggatttatt agtcaagtgt cagattattt attttaaata
     1261 ttctataatt gtattatcca gtaaaagaga tattattaaa atcaataata tctcttttta
     1321 acataaataa ggttgttaaa ttaaggttat tcttataaaa attgtgctta tcagctataa
     1381 acaaggaata aaagtgaaaa aattgctctg agattattac agttaaaaga gttttaagat
     1441 gacagagaga tagattgtca actgtatatt taataaatct tgttatttat taaattttat
     1501 ataagtaaaa tatataaatt aataaatctt atgctagaat atatatgaaa aacaatataa
     1561 atttggagga ttaaaaacat gacagagcga gaaaaaatgt tggcaggaga actttacgat
     1621 tgtggtgata ttgagttgtt aactcaatgg cataaagcaa aagacctagt aagagattat
     1681 aaccaaacag attcctcgga tttggaaaaa aaagaaaaaa tattaaatga gttacttggt
     1741 ggtaagggta aaaacttatg gattactgca ccatattatg tagattatgg aaataatatt
     1801 tattttggaa ataattgtga agtcaatatg aattgtacgt ttttagatga taataaaatt
     1861 atcattgggg ataatgcact aatcgcacca aacgtacaaa tatatactgc atttcatcca
     1921 actaatgctg gtgatagatt tggtaatgcc aaagaagatg gttcatttga attttgtaaa
     1981 actcaaacag ctccagttac tattggagat aatgtttgga ttggcggggg tgctattatc
     2041 atgccaggtg ttactattgg agataatgta gttataggtg caggaagtat tgttacaaaa
     2101 gatattccaa gtaatatgat tgcatacgga aatccatgcc gtattattcg agaaaataaa
     2161 taaagttaaa ttttcttgca aaatctcaaa aaataccttt cttactttat acatagggta
     2221 ttttctaata ctttcacatc catattcatt tgttaaattt ttatggaatg tagtttttat
     2281 catctctatc ttacattttc actatttttt tataaaagtc attcctttat agatttattt
     2341 ataatagtat atattctatt tgatgtttga ataagcactt atttttaagt aaaatactaa
     2401 cttttaggtt agtattttgc tatagtatga tgattaagta agaataaaat actataatac
     2461 ataaagctta acaaatataa tttttatttt acttagatat tttcttttga ttagcaatca
     2521 aaagtggtta taattttttt aattaataag agtaac
//
LOCUS       DAELLV010000342         2500 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.343, whole genome shotgun sequence.
ACCESSION   DAELLV010000342 DAELLV010000000
VERSION     DAELLV010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.343"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            261..2060
                     /locus_tag="KPD58_003548"
     CDS             261..2060
                     /locus_tag="KPD58_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG0267271.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKTEGVITVVESMGQIQVVIGNKVKKVYDEVIKIVPQSNNIGKKNESQEKQGIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYVEKVLEKYIPEVIKIIMVPTLSLVVMLPATLFLFGPIGIYIGNVI
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGAA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AFGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSSIKVETKDGVIDITSPVEGECIELSEVKDDVFASKAMGEGIAVLP
                     TKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKKAGYDVTTPVIVNNTFD"
BASE COUNT          918 a          304 c          490 g          788 t
ORIGIN      
        1 aaaaaaaatt taaatatata attattattt acaaaaaaat tatactttat agttgaatta
       61 agaaaacgct tatgatacta taaagttaac aaatttgaat atataacagt agacgtgtta
      121 ctggtaatgc aggcaagatc gaatcaattt ttatagatta tctatagagt actatgggta
      181 taatctattt atttggttcg atttttttgt ttaattggcc ataaaacaaa aagcctatag
      241 ttgaaaatgg aggatttttt atggattata aaaatatagc tcaagaaatt ctactaaatg
      301 taggaggaaa agagaatgta aatgaagtga ctcattgtat gacaagactt agatttaaag
      361 tcaaaagcgc ttctaaagtg aataaggata aattatctaa aactgaagga gtaataactg
      421 ttgttgagag tatgggtcaa atacaagttg taattggaaa taaagtaaaa aaagtatatg
      481 atgaggtcat aaaaattgta cctcaatcaa ataacatagg taaaaagaat gaaagtcaag
      541 aaaagcaagg tataattaat tctatattat cagcagtagc tggtatattt acacctacaa
      601 tacctgctat agcaggggtt ggtatgatta aaggtatttt atcagttctt gctatgtatt
      661 atatgaataa aaatggagta gatataaaag aaacgcaaag ctatattatc ttaaatgcta
      721 tggctgattc tatattctat tttatgccta ttatactagg atatactgcg gctaaagtat
      781 ttaatgcaaa taaaataata tctatggtat taggagctac actttgttat ccaactttta
      841 ctgctttgat ggcaggtgaa gaatctgtta gatttttagg attggcagta acaaaagcta
      901 cgtatacatc atctgttatt ccaataatta ttgctatatg ggctctttca tacgttgaaa
      961 aagtattaga aaagtatata cctgaggtaa ttaaaataat aatggttcca actttatcat
     1021 tggtagtaat gttacctgca acattatttt tatttggacc aatcggaatt tatataggaa
     1081 atgtcataaa cttttcttat aagtatattt atgaattaag cccagcattg tgtggagcat
     1141 ttgtaggtgg gttatggtgt gtgttagtta tatttggtgc tcatagagca ttattaccaa
     1201 taggtataag tgatgttgct caaacaggaa gacaaaattt attggcgttt gcaggagctg
     1261 caaacttttc acaagcaggg gctgctctag gagtattctt taaaactaaa aatcaaggac
     1321 taaaaacaat ttctatgtct gcaactataa cagctttatt tggtataact gaacctgcaa
     1381 tatacggtgc caatcttaga ttaaaaaaac ctatggtatg tgccgtaata tgtggtgcat
     1441 ttggaggtgc gataatggga atgggtggag cttatggtaa tgcatttgca aaccaaggtg
     1501 ttttaactat acctgtatat gctgaagcag gtgcattagg tttcttaagt tatctaggtg
     1561 gttgtgctat agcatttttt ggttcagcta tttcaactta tctagttgga tttgaagatt
     1621 tggaagagtc ttctaataag gaaaattcat ctataaaagt agaaacgaag gatggtgtta
     1681 ttgatataac ttcacctgta gagggcgaat gtatagaatt gtcagaagta aaggatgatg
     1741 tatttgcttc aaaagctatg ggagaaggta tagctgtgtt acctacaaaa ggagtaataa
     1801 cagcccctac tgattgtgaa gtagcttctt tatttccaac attacatgca ataggattaa
     1861 aattagataa tggagcagaa atgttaatac atgttggaat caatacagta gagttaaatg
     1921 gtaaatactt tacaaaacat gttaatcaag gagatttagt gaaaaaaggt gataaactta
     1981 tatcctttga tatagataaa ataaaaaagg ctggttatga tgtaactacg ccagttatag
     2041 taaacaatac atttgattaa aaaatagcta cgcacactcg tgactttagt catgagttag
     2101 tagctaaaat agtcagcata tagggaaacc tatatgtaga gataggatag aacctatcca
     2161 atactacttg aattgctgga aacccctaaa gctaaccaaa ctaaaacata aggatgaaat
     2221 aaacctaagt gtgaaagtta cgaaagtaga aaaaattggt tagatggtat aaggttaaat
     2281 cctaagtact ttaaaatggg caatcagcag gtaaactccg aataggagaa acttcaacga
     2341 ctattcctct tgagggaagt acactacaag ttatcggtag tggaagtggg tagaccttaa
     2401 cggataatgc cgaaggataa gatatagtct gtgcttgcat gaaagtgtaa gaagttcata
     2461 agagaactgc ataggtagta gcgaacttat gtgaacgaca
//
LOCUS       DAELLV010000343         2498 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.344, whole genome shotgun sequence.
ACCESSION   DAELLV010000343 DAELLV010000000
VERSION     DAELLV010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.344"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..176)
                     /locus_tag="KPD58_003549"
     CDS             complement(<1..176)
                     /locus_tag="KPD58_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0267272.1"
                     /translation="MREIKAKFYRSGDIVRDIGIVYFYELLMDLKNELEKNDYKLKFT
                     CELNRNYLSLESAE"
     gene            complement(181..918)
                     /gene="cas6"
                     /locus_tag="KPD58_003550"
     CDS             complement(181..918)
                     /gene="cas6"
                     /locus_tag="KPD58_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HBG0267273.1"
                     /translation="MSRISIFMESCNGKFPNENSMLSVSFIKNILNAENKDFAKNIFN
                     YGKERKNNKQIKDINTAIYIPNLIRSKNELLVDGDIIFNISFYNYSMFSKLYNGILKV
                     KELEYKGYRFKIKNIKIHKEHEIKENGVIFKTMSPIIVKNKEGKYLDVEDSNYIECLN
                     YIANLTLESIRGSGLRKPLEFIPLNFKKRVLKEKIRGFKVREFYYINAYAGTFFLKGD
                     MEDLNALYKMGIGYRRTENAGMVDILK"
     gene            1176..2285
                     /gene="ugpC"
                     /locus_tag="KPD58_003551"
     CDS             1176..2285
                     /gene="ugpC"
                     /locus_tag="KPD58_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HBG0267274.1"
                     /translation="MSEVILKNISKLYSNGFNAVKNINIDIKDKEFIVLVGPSGCGKS
                     TTLRMIAGLEEISEGELYIGDKLVNDIEPKDRDIAMVFQNYALYPHLSVYENMAFALK
                     LRKLPKDEIDKKVKEAAKILDLLPLLNKKPKTLSGGQRQRVALGRAIVRNPKVFLMDE
                     PLSNLDAKLRTAMRTEITKLHQQLGTTFIYVTHDQVEAMTMADRIVVMKDGVVQQIAT
                     PQDVYDYPANIFVAGFIGAPQMNFIDVILIEENDEIYAQNEHIKLKLNKEKHYDLIKD
                     NYINNEVVIGIRPEDIHVEDTFIKSNPDTCFKSRIEIKELMGAETYAHLKLGENTITI
                     RFDSKNRINVGDDLTLSVDNSRVHIFDKETTLAIR"
     gene            2315..>2498
                     /locus_tag="KPD58_003552"
     CDS             2315..>2498
                     /locus_tag="KPD58_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HBG0267275.1"
                     /translation="MEELKDFLERQLNKKINIYPYENQKLDASSHLVTFNNAIYVIDF
                     LDKNNLDNLKGNFYLIY"
BASE COUNT          931 a          352 c          296 g          919 t
ORIGIN      
        1 tattctgcag attctaaaga taaataattt ctatttaact cacaagtaaa ttttagctta
       61 taatcatttt tttctagttc gttttttaaa tccattagta attcataaaa gtaaacaatg
      121 ccaatatctc ttacaatatc acctgatcta taaaatttag cttttatttc tctcacctcg
      181 ctactttaat atatcaacca ttcctgcatt ttccgtcctt ctatatccaa ttcccatttt
      241 atacagtgca tttaaatctt ccatatctcc ttttaaaaaa aatgttcctg cataagcatt
      301 tatatagtaa aattctctca ctttaaatcc acgaatcttt tcttttaaca ctcttttttt
      361 gaagttaaga ggtataaatt ccaatggctt tctaagccca ctacctctta tactctccag
      421 agttagatta gctatatagt ttaaacattc tatataattt gaatcttcta catctaaata
      481 tttaccttct ttattcttta ctattatagg agacatagtt ttaaatataa caccattttc
      541 ttttatctcg tgttctttat gtatttttat attttttatt ttaaatctat aacctttata
      601 ttctagctcc tttactttta aaattccatt gtataattta ctgaacatag aataattata
      661 aaaacttata ttgaatatta tatctccatc aacaagtaac tcatttttgc ttcttataag
      721 atttggtatg taaatggctg tatttatatc ctttatttgc ttattgtttt ttctttcttt
      781 cccatagtta aatatgtttt tagcaaaatc tttgttttct gcatttaaaa tattttttat
      841 aaaggaaact gacaacattg aattttcatt tggaaatttg ccattacatg attccataaa
      901 aattgaaatt ctgctcatat tttcacctcc tactttctag tataattcat tattctctat
      961 ttgtaatata aaaataaaac atatcataaa atgatttttt gataataaat ttaattttgt
     1021 tttttaattt tttaaatata ctttactgtt agttttattc tttgctaaaa actaaatata
     1081 catattaaac aaatacattt agtcatttta cacatttaat ataacaaaaa aatttgatac
     1141 tatataatta aaatttaaat atcaaggagg atattatgtc agaagtaatt ttaaaaaata
     1201 tatcaaaact ttactcaaat ggatttaacg cagtaaaaaa tataaatata gacattaaag
     1261 ataaagaatt tatcgtttta gtaggaccat ctggttgtgg taaatcgact acacttagaa
     1321 tgattgctgg tcttgaagaa atctctgaag gagaactata cattggagat aaacttgtca
     1381 atgatataga accaaaagat agagatatag ctatggtttt tcaaaactat gctttatatc
     1441 cccatttatc tgtttacgaa aatatggcat ttgcacttaa acttagaaaa ttacccaaag
     1501 atgaaataga caaaaaagtt aaagaagctg ctaaaatatt agatttacta cctcttttaa
     1561 ataaaaaacc taaaacatta tctggaggac aaagacaaag ggttgcctta ggacgtgcaa
     1621 tagttagaaa tcctaaggta ttcttaatgg atgaacctct atccaactta gatgcaaaac
     1681 ttagaactgc tatgagaaca gagataacta agctacacca acagcttgga actacattta
     1741 tctatgtaac acacgaccaa gttgaagcta tgactatggc tgatagaatc gtagtaatga
     1801 aggatggagt agttcagcag attgcaactc cacaagatgt atacgattat ccagctaata
     1861 tatttgttgc aggatttatt ggagcaccac agatgaattt tattgatgta atattaatag
     1921 aagaaaatga tgaaatttat gcacaaaatg aacatattaa gcttaaattg aataaagaaa
     1981 aacactatga tttaataaaa gacaattata ttaataatga agttgttata ggtatcagac
     2041 cagaagatat tcatgtagaa gatactttta ttaaatctaa tccagatact tgttttaaat
     2101 caagaattga aataaaagaa cttatgggag cagaaactta tgctcactta aaactaggcg
     2161 aaaatacgat aacaattaga tttgacagta aaaacagaat taatgttggt gatgacttaa
     2221 cactttcagt tgacaatagt agagtacaca tatttgacaa agaaactact ttagctataa
     2281 gataagaata taatcttatt tttaaggaga ctttatggaa gagttaaaag attttttaga
     2341 aagacagctt aataaaaaaa taaatatcta tccatatgaa aaccaaaagt tagatgctag
     2401 tagccattta gttactttca ataatgctat ttatgttatt gattttctag ataaaaataa
     2461 tctcgataat ttaaaaggca acttttattt aatttacc
//
LOCUS       DAELLV010000344         2475 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.345, whole genome shotgun sequence.
ACCESSION   DAELLV010000344 DAELLV010000000
VERSION     DAELLV010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.345"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(51..1013)
                     /locus_tag="KPD58_003553"
     CDS             complement(51..1013)
                     /locus_tag="KPD58_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBG0267276.1"
                     /translation="MKKGYVYIILTTLFFSSMEISLKTVTNDFNPLQITLSRFFVGGL
                     VLLPFAVKRLRALSLSITKSDLKYFAFLGFMCVVVSMSLYQLAVLNTKASVVAVLFSC
                     NPVFVMLLAYLILKEKIYRHNVISLILEVLGIIVIINPLHTKLTLSGIFLTLSSAIIF
                     SMYTVFGKRKTLKFGGIVVTCFSFIFGSLEMLILVLLTKINFIADILNKNKLHVFANI
                     PIFSGYTLHNMPIMIYVFVFVTGVGYALYFMAMEATSTSLTSLVFFFKPVISPILALL
                     ILKEIIPINMVIGILLIVIGSIISIIPTIITQMHNKHVEALYKE"
     gene            complement(1183..2373)
                     /locus_tag="KPD58_003554"
     CDS             complement(1183..2373)
                     /locus_tag="KPD58_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG0267277.1"
                     /translation="MISNKMQTLVANSSVIRAMFEEGKKLSDIYGEENVFDFSIGNPS
                     VEPPETIKAVINDILNEESPNLVHGYMNNSGYEDVRDAIAEHINKKDGLNLTRENLIM
                     TCGAAGGLNIILKTLLNPGDEVIAFAPYFGEYKNYTENYDGKLIEVPTNIETFEPDLD
                     ALKNAITPKTRALIINTPNNPTGVIYSEELLKNLGELLDSKQKEFNTSIYLISDEPYR
                     EIIYDGAKVPCVLKYYRNSFIGYSYSKSLSLPGERIGYIVANGQMDDFDDVMSSLNVA
                     NRILGFVNAPSLFQRVIARSLDAEVDVNIYKKNFDLLYNSLIDMGYSCVKPNGTFYLF
                     PKAPIEDDKKFCNDAKQFNLLLVPGSSFGCPGHFRVSYCVSYDKVKSSLPAFEKLAKL
                     YNLK"
BASE COUNT          927 a          363 c          302 g          883 t
ORIGIN      
        1 taaatctaat aaattttgtc ttatgtataa ctatgataga ttatcaatta ctactcctta
       61 tacaaggctt caacgtgttt attatgcatt tgagtaatta tcgtaggtat aatagaaatt
      121 attgaaccaa ttactatcaa taaaattcct ataaccatgt ttatagggat aatttcttta
      181 agtattaata atgctagtat tggagatatt actggtttaa agaagaatac taaagatgta
      241 agactagtag atgtagcttc cattgccata aagtaaagcg cataaccaac acctgtaaca
      301 aatacgaata cataaatcat aatgggcata ttgtgtaagg tatatccact aaaaataggg
      361 atatttgcaa atacatgaag cttattttta tttaatatat ctgcgataaa attaatttta
      421 gtgagtaaaa ccaatatcaa catttccaaa ctaccaaata taaagctaaa gcaggttaca
      481 actattccac caaattttaa ggtttttctt ttaccaaata cagtatacat tgaaaaaata
      541 atagcagaag ataatgttaa gaatattcca ctaagagtta gttttgtatg caatggattt
      601 attataacta taattccaag aacttctaat ataagtgaaa ttacattgtg cctatatatt
      661 ttttccttca aaattaagta agctaaaagc attacaaaga ctggattaca actgaacaaa
      721 acagcaacta cagatgcctt tgtgtttaga acagcaagtt gatacaatga catacttact
      781 actacacaca taaaacctaa aaatgcaaag tattttaagt ctgattttgt aatagaaaga
      841 ctaagagctc ttaatctttt tacagcaaaa ggtagtaaga ctaaaccgcc aacaaaaaat
      901 cttgataaag ttatttgtaa gggattaaag tcattagtga cagtttttag actaatttcc
      961 attgaactaa aaaataaggt agtcagtatt atataaacat aacctttttt caagtcaaac
     1021 acctccttgt aatttatatt agtactatag ataagaatat tcaagcatat tggaataata
     1081 tggtatgtat aaaatatagt acataaaatt ataaactacc ttaatataga atgtaagtat
     1141 tctatactaa ggtagttaat tatttgaatt actttgattt agttatttta aattatataa
     1201 tttggctaat ttttcaaatg ctggtaaaga acttttaact ttatcataag atacacaata
     1261 tgatactcta aaatgacctg gacaaccaaa agaagaacct ggtactaata ataaattaaa
     1321 ttgttttgca tcattgcaga attttttatc atcttcaatt ggagcttttg ggaacaaata
     1381 gaatgtacca ttaggtttaa cacaagaata tcccatatcg attaaactat tatatagaag
     1441 gtcaaaattc tttttataaa tatttacatc aacttctgca tctaaacttc ttgcaataac
     1501 cctttgaaaa agagaaggag catttacaaa tccaagtatt ctatttgcaa catttaatga
     1561 agacataaca tcatcaaaat catccatttg cccattagca acaatatatc ctatacgttc
     1621 accaggtaga gataatgatt tactataaga gtatcctata aatgaatttc tatagtattt
     1681 tagtacacaa ggaactttag caccatcata aattatttct ctatatggct catcagaaat
     1741 taggtatatg cttgtattaa attctttttg ttttgaatcc aataattctc caagattttt
     1801 taataattct tcagaatata tgacaccagt aggattattt ggagtgttta ttattaaagc
     1861 tcttgttttt ggagtaatag cattttttag agcatctaaa tctggctcaa aagtctctat
     1921 atttgtagga acttctatta attttccatc atagttttct gtataatttt tatactcacc
     1981 aaaatacggt gcaaaagcaa taacttcatc accaggattt aaaagagttt ttaaaataat
     2041 atttagacca ccagcagcac cacaagtcat aattaaattt tctcttgtaa gatttaatcc
     2101 atctttttta tttatgtgtt cagcaatagc atctcttaca tcctcatatc ctgagttgtt
     2161 catatatcca tgaactaaat ttggagattc ttcatttaaa atatcattta taactgcttt
     2221 aatagtctct ggaggttcaa cacttggatt tcctatacta aaatcaaaaa cattttcctc
     2281 accataaata tcagaaagtt tctttccttc ttcaaacata gctcttataa cagaactatt
     2341 tgcaactaaa gtttgcattt tattagatat catatgtaac actccttttt atagatttat
     2401 attgcttatg tatatattac catattttta ctgaatttag acaattttcg aaaaattact
     2461 tgtttgaaaa tataa
//
LOCUS       DAELLV010000345         2454 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.346, whole genome shotgun sequence.
ACCESSION   DAELLV010000345 DAELLV010000000
VERSION     DAELLV010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.346"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            332..1078
                     /locus_tag="KPD58_003555"
     CDS             332..1078
                     /locus_tag="KPD58_003555"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBG0267278.1"
                     /translation="MDRVAFTLFGIDIMWYGILMACGMILGTLIAIKEAKRVGIKEDD
                     VLNIAIIAIPVGLICARIYYVVFNWSYYAQNMSQIFNFRGGGLAIHGGLIGGILAGYI
                     YTKIKNINFLKMADTVILGMPLAQAIGRWGNFINGEAHGGATNLPWGIMVDGVKVHPT
                     FLYESIWDFGIFIVLLLFRKNKKYEGQVIVTYITLYSIGRFFIEGLRTDSLMLGPLRM
                     AQVISLIGVIGGIIAHVYLSKKNKNNISEE"
     gene            1108..2043
                     /gene="rsmH"
                     /locus_tag="KPD58_003556"
     CDS             1108..2043
                     /gene="rsmH"
                     /locus_tag="KPD58_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBG0267279.1"
                     /translation="MEFHHVSVLLNECIENLNIKPDGVYVDCTMGGAGHSKEIVKKLS
                     DKGLFIGFDQDKNAISTAKERLSEYESRVKFVHSNFENIKEELEKIGVYKIDGVLADL
                     GVSSHQLDEADRGFSYMQDAPLDMRMDVRCEFSAYDVVNTYTEDELTKIIKDYGEDNW
                     AKRIAKFIVEERANKPIETTGELVDVIKKAIPKKARIDGPHPAKRTFQAIRIEVNNEL
                     GVITKMINDASSIMNEGGRICIITFHSLEDRIVKNAFKHLASDCICPQHLPICQCDKE
                     SEVKIITRKPILPSEEEIEVNPRSRSAKLRVAEKI"
BASE COUNT          987 a          211 c          461 g          795 t
ORIGIN      
        1 ggtaaaacaa atttataaaa tatatttata taaattgagg taaaaattta agagctatag
       61 ataaggaata gaagaggttg atgtatatat gcatcaatct ttttgattaa aaaatatcta
      121 tgtacacttg taactttagt catgagagat tcagtttaaa aaattttata gtaattagat
      181 ataatatagt gtagggagtt gtagaataaa ttttaatatt ttttgtagaa ttaacattaa
      241 tgtgttatca taatttagta taattacaat taattgtaat tatagcatat tgctttggaa
      301 atattttcaa ttaatacaaa gaggtgacgt tatggataga gtagcattta cactgtttgg
      361 catagacatc atgtggtatg gaatacttat ggcatgtgga atgattttag gaactttaat
      421 agcaataaaa gaagcaaaaa gagtgggaat taaagaagat gatgtattaa atatagctat
      481 aatagcaatt ccagttggac taatctgtgc aagaatctat tatgttgtat ttaattggag
      541 ttactatgct caaaatatgt ctcaaatttt taattttaga ggtggtggac ttgctataca
      601 tggagggtta attggtggaa tattagctgg ttatatatac actaagatta aaaatattaa
      661 cttcttaaaa atggctgata cagttatact tggaatgcct ttagcacaag ctattggaag
      721 atggggaaat tttattaatg gagaagcaca tggaggtgct accaatttac cttggggaat
      781 aatggttgat ggagtaaagg tacatccaac ttttttatat gagtctatat gggattttgg
      841 aatatttata gttttattac tatttagaaa aaataaaaaa tatgaaggtc aagttatagt
      901 tacttatata actttatatt caataggaag attttttata gagggattaa gaacagatag
      961 tttaatgtta ggaccactta gaatggcaca agttataagt ctaattggag ttattggtgg
     1021 cataattgca catgtttatt tatcaaagaa aaataaaaat aatattagtg aagaatagtt
     1081 taaaaagtga atttaaggga ggtaactatg gaatttcatc atgtatcagt tcttttaaat
     1141 gagtgtatag agaatttaaa tataaagcca gatggcgtgt atgttgactg tactatggga
     1201 ggagcaggac attctaaaga aatagtaaaa aaactgtcag ataagggatt atttataggg
     1261 tttgaccaag ataaaaatgc tataagcaca gcaaaagaga gattgagtga atatgaaagt
     1321 agagtgaagt ttgttcacag caattttgaa aatataaaag aggaattaga aaaaatagga
     1381 gtttacaaaa ttgatggtgt attagctgat ttaggagttt catctcatca acttgatgaa
     1441 gctgatagag gattttctta tatgcaagat gcaccacttg atatgagaat ggatgttagg
     1501 tgtgagtttt ctgcatatga tgttgtaaat acttatacag aagatgaatt aactaagata
     1561 ataaaagatt atggagaaga taactgggct aagcgtatag ctaaatttat agttgaagaa
     1621 agagcaaaca aaccaataga aactacagga gaattagtag atgtaattaa aaaagctatc
     1681 cctaagaaag ctagaataga tggacctcac ccagctaaaa gaacttttca ggcaatacga
     1741 atagaagtaa ataatgaact tggagtaatt acaaaaatga taaatgatgc ttcttctata
     1801 atgaatgaag gtggaagaat atgtataata acattccatt cattagaaga tagaatagtg
     1861 aaaaatgcat ttaagcattt agcatcagat tgtatatgtc cacaacattt acctatatgc
     1921 caatgtgata aagaatctga ggtgaaaata ataactagaa aacctatatt acctagtgag
     1981 gaagaaatag aagtaaatcc acgttcaaga agtgctaagc taagagtagc agaaaaaata
     2041 taagtagtta taaggctaaa gaataatctt atatataaga ttattataat taattaaaaa
     2101 gtattatata ttttgttgca ctggtaaagc aattaaaaaa caattaacac tataagaaat
     2161 gttctttgtt attgagatat ataatgaaaa tttagatata aaaatataga tatagatggt
     2221 attaagtaat ataatggaga acttaggaat cagagagtag atagctagtt acatattata
     2281 tttatggaag aaattatcta aaaatactag aagtgataaa aataaataga aattaatatc
     2341 taacctatag attcatataa aagtataagt gaatttatag gtttttgttg tgtatagaaa
     2401 aaaatacaat tagattgtgt aatagtgtat taatttagcg atgaaatttt agag
//
LOCUS       DAELLV010000346         2424 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.347, whole genome shotgun sequence.
ACCESSION   DAELLV010000346 DAELLV010000000
VERSION     DAELLV010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.347"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(594..1145)
                     /locus_tag="KPD58_003557"
     CDS             complement(594..1145)
                     /locus_tag="KPD58_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0267280.1"
                     /translation="MSINTVIAKNLNRLRNERNLSLGQLAELSGVSKVMLSQIEKGDS
                     NPTVNTIWKIASGLNVPYTAILEQPQNETFIVSKTDIDVQVSENKDYRLYCYYPNTPT
                     RNFELFQMELEEGHSYTSVGHSEKSQEYIMIIEGQLKLEVNDSIYQLRENDSICFSAE
                     SIHTYHNQGEKTLKAVIINYYPV"
     gene            1336..2298
                     /locus_tag="KPD58_003558"
     CDS             1336..2298
                     /locus_tag="KPD58_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HBG0267281.1"
                     /translation="MRVLNNVTEAIGNTHMLHLSKIADEFGVQGNIYAKMEYLNPGFS
                     KKDRVALQMITEAENAGLLVPGQAVVELTSGNTGTGLAIACACKGYKFIACMSKGNSM
                     ERARMMKALGAEVVLVDQMPNSVHGQVSGEDLALVEIEAQKIAKERNAFRADQFNLEA
                     CNHAHEKYTAEEMWEQLDGNIDVFVDFLGSGSTFAGCSKTLKKHNSSIKCYVVEPETA
                     AIYSGKEITDQGHKIQGGGYSMDLPLVDKKLIDGYIQVTDEEATDVARKLAKLEGVFA
                     GFSSGANVCAAIKLLKSSEKGKNIVLTLNDSGLKYLSTDLYEEL"
BASE COUNT          926 a          260 c          402 g          836 t
ORIGIN      
        1 atttttgtca ttaccattat gttttaatat actattcaat tatttaactt cttaaattat
       61 atttagatat aggagtctta tgataaaagt aagcttatgt gttatttgta gtaaatataa
      121 agtgcatata gtggtaaaac aaaagtttca tataaatgaa gaaaaaaata taaaataatt
      181 tatttaacta cttaaaaata aactaaaagg gataataaaa tagcttttag gttatggtaa
      241 taaaggtatt taaaaagagt gcataagcac tccaattgtg agaaatacat acacaaatca
      301 ttattacaac aatgaaaaat gtacatataa gagacgtttt gtatttgttt taagtgataa
      361 tattttaata ctaactaaat aattatcaca atagtaaaaa agaaaaagga gtattttgta
      421 ctccatttgt caaaaatata aaacttttat ataagcttat tataacataa ttgataaaag
      481 tgtaaagata tataatgaat ttataaagaa aaataaaata aaaaagataa atcctaatca
      541 aaggatttat ctttttgtat agaaaataat tattctatta ttaaatggca tggctaaact
      601 ggataataat ttattattac tgctttcaat gttttttctc cttgattatg gtatgtatga
      661 attgattcag ctgaaaaaca aatagagtca ttttctctaa gttgataaat agagtcattt
      721 acttctaatt ttaattgacc ttctataatc ataatatatt cctgtgattt ttcagaatga
      781 ccaacagatg tataactatg accttcttct aattccattt gaaataattc gaagtttcta
      841 gttggtgtat tgggataata acaataaaga cgataatctt tattttctga aacttgtaca
      901 tctatatcag tttttgatac aataaaagtt tcattttgag gttgttccag tattgcagta
      961 tatggaacat ttagtccact tgcaattttc caaattgtgt ttactgttgg gtttgagtct
     1021 cccttttcaa tttgcgaaag cattacttta cttacgccag ataattcagc aagttgacct
     1081 agacttaagt tacgttcatt acgtaatcta tttaaatttt tagcaataac agtattaata
     1141 ctcatagtag ctccttaaaa tgtaaattat tgacaaatat aatttacgaa tgtataattt
     1201 atttaacatt atttataatt tacaaatgta taatataatg aacgttaaat acactgtaca
     1261 tccgtaatat aaattatact gcataaagag ttgtttttca acggatgata gttgagagat
     1321 taagggggaa ttgtaatgcg tgtacttaat aatgttacag aggctatagg caatactcat
     1381 atgttacatt tatccaaaat agcagatgaa tttggagttc aaggtaatat ttatgcaaag
     1441 atggaatact taaatccggg atttagtaaa aaggaccgtg tagcattaca aatgattacc
     1501 gaagcagaaa atgcaggttt attagttcca ggtcaagcag ttgtggaatt aacaagtggt
     1561 aatactggaa caggactagc aatagcttgt gcatgtaaag gatataaatt tatagcgtgt
     1621 atgtcaaaag gaaattctat ggaaagagca agaatgatga aagctttagg tgctgaagta
     1681 gttcttgtag accaaatgcc taattcagtt catggacagg tatctggtga agatttggca
     1741 cttgtagaaa ttgaggctca aaaaatagca aaagaaagaa atgcatttag agcagaccaa
     1801 tttaatttag aagcttgtaa tcatgcacat gaaaagtata cagcagaaga aatgtgggaa
     1861 caattggatg gaaatattga tgtatttgta gattttcttg gaagtggtag tacttttgca
     1921 ggatgttcaa aaacacttaa aaaacataat tcttctatca aatgttatgt tgttgaacct
     1981 gaaacagcag caatttattc aggtaaagaa ataactgacc aagggcataa aatacaaggt
     2041 ggaggatatt ctatggattt gccattagta gacaagaaac ttattgatgg ttatattcaa
     2101 gttacagacg aagaggcaac tgatgtagca agaaaattag ctaaattaga aggtgtattt
     2161 gcaggatttt cttcaggggc aaatgtatgt gcagcaatta agttgttaaa gagcagtgaa
     2221 aaaggaaaaa atatagtttt aactcttaat gatagtggtt taaaatattt aagtactgat
     2281 ttatatgaag aattgtaata atagcacaat agttatgcaa tgttttttaa attaagtata
     2341 tagatttaaa ttataaaaag tatctcaatg tgatttttga aagttataaa ggagatactt
     2401 ttttattttg taaatatgga gtat
//
LOCUS       DAELLV010000347         2421 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.348, whole genome shotgun sequence.
ACCESSION   DAELLV010000347 DAELLV010000000
VERSION     DAELLV010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.348"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(139..894)
                     /locus_tag="KPD58_003559"
     CDS             complement(139..894)
                     /locus_tag="KPD58_003559"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBG0267282.1"
                     /translation="MLKVIISGYNGTMGRVLAECVKKDNELELVCGVARSADEKGHHS
                     DTKLYKTMTDVAEKTDVIIDFSHHETLEPILSYALKNNTPVVLATTGYDENDLNKIEE
                     ASKNIPILLSSNTSLGVNILIKLVKDASKMLEGFDIEIIEKHHNRKEDAPSGTATMIA
                     NAIREVLPESENTHGRYGRDCKRKSNEIGIHSIRGGNIISDHDVVFAGDNEVLTLSHH
                     SQSDEVFANGSIVAAKFLIGKQSGLYKMSDILG"
     gene            1334..2323
                     /locus_tag="KPD58_003560"
     CDS             1334..2323
                     /locus_tag="KPD58_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBG0267283.1"
                     /translation="MLKEEKTYQEKDAKVFNGVAALIMILLFLVVSIGIMVFGIVKLD
                     SGFTGIGSLMLILGLVFIVVDFILFFGLKMINPKEAIVLVLFGNYYGTIKKEGYYWVN
                     PFCSAINPAVSRINVSKSSSDEKVDISTNSSGKKVSLKTMTLNNEKQKVNDLLGNPII
                     IGVVVIWKVIDATKAVFNVDNYNTFLSIQCDSTIRNVSRLYPYDVSEDGDEKSLRGSS
                     QEIADRLKDELQSRVDIAGIEVCEVRITHLSYAPEIAAAMLQRQQAEAIIAARKKIVE
                     GAVSMVEMALTNLSENNVVTLDEERKAAMVSNLLVVLCGNKDAQPVVNSSSIY"
BASE COUNT          878 a          307 c          361 g          875 t
ORIGIN      
        1 aatctttaag aaaaatgatt atatttaaaa ggtaaacata aaatttataa acttttagaa
       61 tagaaatctc aatcaattag atattcaaat tgatgaatat gaatacctaa atagattgag
      121 atttattatg tacaaaactt atcctaatat atctgacatt ttataaagcc cagattgctt
      181 accaattaaa aatttagctg caactataga accgtttgca aatacttcat cagattgaga
      241 atgatggctt aatgtaagaa cttcattatc acctgcaaat acaacatcat ggtcgctgat
      301 tatattgcca cctcttatag agtgtattcc aatttcattt gattttcttt tacaatctct
      361 accatatcta ccatgtgtat tttcactttc aggcaagact tctctaattg cattagctat
      421 catagtagca gttccactag gggcatcttc ttttctattg tgatgttttt ctatgatttc
      481 aatatcaaaa ccttctaaca tctttgaagc atccttaact aattttatta atatattcac
      541 tccaagagat gtatttgatg ataataatat aggaatgttt tttgatgctt cttctatttt
      601 atttaagtca ttttcatcat atccagtagt agctaatact acaggtgtat tattttttag
      661 agcatatgac aatattggct ctaaagtctc atgatgtgaa aagtctataa ttacatcagt
      721 tttttcagct acatcagtca ttgttttgta aagctttgta tcactatgat gacctttttc
      781 atcagcagac ctagctacac cacaaaccaa ctctaattca ttatcttttt ttacacattc
      841 tgctaaaact cttcccattg ttccattata gccactaatt ataactttca acataaattc
      901 ctcctatgaa attaataaaa atttgttata tttctataag tttattttat cactaatata
      961 atttatataa aataatatta gtgataataa gcttacaatt tatcttacat taaaaaattt
     1021 aagtggattt ttcaaaatat agataaattt ctttttcatt gtataaatga taaaaatata
     1081 tttgacttca taccaaaaag agtgccccat gaactaaaaa gttaattttg aaataccata
     1141 ttcatatatc atatgattta tttggaaatc aatattaata cattaaaata actaaataca
     1201 tgtatgcata aagatgtata aaaaatatat aaacatatag tattaatttt aaattcaaca
     1261 atttaattgt taaaagtgtt attattaaat tagaaatatc ttaacttaat atactgagat
     1321 gggagtgata gatatgttaa aagaagaaaa aacttatcaa gagaaagacg caaaagtatt
     1381 caatggagtg gcagctttga taatgatttt attattttta gtagtttcaa taggtatcat
     1441 ggtttttggg atagtaaaac ttgatagtgg atttactgga ataggttcac ttatgttaat
     1501 tttgggactt gtatttatag tagtagattt tatcctattt tttgggctaa aaatgattaa
     1561 tcctaaagaa gctatagttc tagttttgtt tggtaactat tatggaacta taaaaaaaga
     1621 agggtactat tgggtgaatc cattttgttc agcaataaac ccagcagtat caagaattaa
     1681 tgtatcgaaa tcttcatctg atgaaaaggt agatatatca actaattcaa gtggcaaaaa
     1741 agtatcttta aaaacaatga ctcttaacaa tgaaaagcag aaagtcaatg atttattggg
     1801 aaatccaatt ataataggag tggttgtaat ttggaaggtt atagatgcaa caaaggcagt
     1861 atttaatgtg gataattata atacattctt atctattcag tgtgattcta ctattcgcaa
     1921 tgtttcaaga ctttatccat atgatgtatc tgaagatgga gatgaaaaat ctcttcgtgg
     1981 tagtagtcag gaaatagcag atagattaaa agatgaatta caatcaaggg ttgatattgc
     2041 aggaattgaa gtgtgtgaag tgagaattac tcatctttct tatgcaccag agattgcagc
     2101 agcaatgtta caacgtcaac aagcagaggc tataatagca gcacgtaaaa agattgttga
     2161 gggggctgta agtatggtag agatggcatt gactaattta agtgaaaata atgttgtgac
     2221 tttggacgaa gaacgtaaag cagctatggt aagtaatctg ttagttgtgc tttgtggcaa
     2281 taaagatgca caaccagtag taaacagcag ttctatttat taaattataa aaagtttaat
     2341 agtttatttt attgatatat agaaaattta gtagttcatt ttattaaatt atagaaagat
     2401 taatggtttg ctttattgaa t
//
LOCUS       DAELLV010000348         2416 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.349, whole genome shotgun sequence.
ACCESSION   DAELLV010000348 DAELLV010000000
VERSION     DAELLV010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.349"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            95..1051
                     /locus_tag="KPD58_003561"
     CDS             95..1051
                     /locus_tag="KPD58_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG0267284.1"
                     /translation="MENNTIQKQLRDLSKKIWHIYILNPEEENLKFIISLLDDNLALI
                     GTGKHEFYETLEEFIIGLEKDQTESEQIDFEIIDEWYSCTKVTDEAFIVYGTLWVIEK
                     DYLEKDIYVEMDTRFTMVYRVTDDMIKLIHVHHSIPNFDQMKDEYYPKTITEKAQEAL
                     AMANEFKLKSEKDLMTGLYNRHFFEVHIKKMLKTVSHGYFYIFDLDNFKQVNDIFGHV
                     RGDEILIHFSNILTKVFVNDAIIGRLGGDEFAVFEYSNQSKENVENKAKKVLELCLES
                     NSCSIGISKLENNSDTFQSLYKQSDYALYYSKRNNKGNFSWF"
     gene            complement(1453..2343)
                     /locus_tag="KPD58_003562"
     CDS             complement(1453..2343)
                     /locus_tag="KPD58_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0267285.1"
                     /translation="MKKNILTTVITMIIILMMSMTVMAEPYQEDETSSLKKQSANERN
                     QTNIGTMDGRVQTNAISKGDNYDPKYPLKGMLTQLGLNTTGKEGWDLLYGGNPNGVEL
                     RADGKYYYINKGMEGLGFYAAALSGGSYQRIGFGKLDPIEVIADGNWISYKQAQDTLT
                     VIRNFLNSFDWRNASEMERANRAAKLVTEAKYADSKYCNIVYGNLVDKRGVCGSFASS
                     FHLLTRLMGMDSLSILNPSLNHAWNYIQIDGKWYRFDGSEISAFGGSLDFDYRKLKDA
                     TREMTTYYDAKALSILGFNQ"
BASE COUNT          884 a          378 c          307 g          847 t
ORIGIN      
        1 taatttcttt agttctatat tataataata ttgtaataag agttcaactt ataatacatt
       61 aaaatataaa ctgtaaatct taagggggta atctatggaa aataatacaa tacaaaaaca
      121 attaagagat ttatcaaaaa aaatttggca tatttatatt ttaaatccag aagaagaaaa
      181 ccttaaattt attatttcct tattagatga caaccttgca ttaattggaa caggaaaaca
      241 tgaattttac gaaacattag aagaatttat tataggtctt gaaaaagacc aaacagaatc
      301 agagcaaata gactttgaga ttatagatga gtggtattca tgtaccaaag tcacagacga
      361 agcttttata gtatatggta ctctttgggt aatagaaaaa gattacctag aaaaagatat
      421 ctatgtagaa atggatactc gatttactat ggtatacaga gttacagatg atatgattaa
      481 acttatccat gttcaccatt ctattccaaa ctttgaccaa atgaaagatg agtattaccc
      541 taaaacaata actgaaaaag cacaagaagc actagcaatg gcaaatgaat ttaaactaaa
      601 atcagaaaaa gaccttatga ctggactata caatagacat ttttttgaag tacacataaa
      661 aaaaatgctt aaaactgttt cacatggata cttttacatc tttgatttag ataattttaa
      721 acaggtaaat gatatctttg gtcatgttcg aggagatgaa atactaatac atttttcaaa
      781 tattttgact aaagtattcg taaatgatgc tattattgga agacttggtg gagatgaatt
      841 tgctgttttt gaatatagta atcaatcaaa agagaatgtt gaaaataagg caaagaaggt
      901 tttagaatta tgcctagaga gtaattcttg ttctataggt atatctaaat tagaaaataa
      961 ctctgatact ttccagtctt tatacaaaca atcggattat gctttgtatt attcaaaacg
     1021 aaataataaa ggaaacttta gttggtttta gtacaaaaga tattaaaata ggcaaattta
     1081 ttagagtatc ttgtcctata tactaataag taaagtaaac aacaagctat ctttgcatat
     1141 atttatataa accaactatt cttaataaaa ctacaatagt caacatgacc actattatag
     1201 aatataataa actaaaaaca ttattaacat gattaatatt atattttaat aattttattt
     1261 ttaaaagtaa gtccaaaatg ttatatgttt tgattaaaac tgatgttgat ataaaaaatt
     1321 ttacttaaaa aaacacttag aaaggcttta taaagagcct tttttgcttt tattgattaa
     1381 attttcatat aaaattcctt attaaatttc aaatcaataa aaaaaggctc tataagaagc
     1441 ctccccctat ttttattgat taaatcctaa tatagacaat gcctttgcat catagtaagt
     1501 agtcatctct cttgttgcat cctttaattt acggtaatca aagtctaatg atccaccaaa
     1561 agctgatatt tcagacccat caaacctata ccatttccca tcaatttgta tatagttcca
     1621 agcatgattt aatgatggat ttaatataga tagtgaatcc attcccatta accttgttaa
     1681 taaatgaaag ctacttgcaa aacttccaca tacaccacgt ttatcaacca gattaccata
     1741 gactatgtta caatatttag agtctgcata ttttgcttcg gttaccaatt ttgcagctct
     1801 attagctctt tccatctcag atgcatttct ccagtcaaaa ctatttaaaa aattccttat
     1861 tactgtaagt gtatcttgag cttgtttgta agaaatccaa tttccatcag ctattacttc
     1921 tatagggtca agttttccaa agcctattct ctgatatgag ccacctgaca atgctgccgc
     1981 ataaaatcct agcccttcca tacccttatt tatataataa tatttaccat ctgctcgaag
     2041 ctcaactcca ttgggatttc ctccatagag taaatcccat ccctctttac ctgttgtatt
     2101 aagaccaagt tgtgttaaca ttcctttaag aggatattta gggtcataat tatcaccttt
     2161 acttattgca tttgtttgca ctctgccatc cattgtgcct atattagtct gattacgttc
     2221 atttgcactc tgttttttca agctagatgt ttcatcttct tgatatggct ctgccattac
     2281 cgtcatactc atcatcaata taataatcat ggtaatcaca gtagttaata tattcttctt
     2341 catactactc ccccttattt aattcttttt aaaacataaa attattaact cataaatata
     2401 tggttattag taaatt
//
LOCUS       DAELLV010000349         2385 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.350, whole genome shotgun sequence.
ACCESSION   DAELLV010000349 DAELLV010000000
VERSION     DAELLV010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.350"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..586
                     /locus_tag="KPD58_003563"
     CDS             <1..586
                     /locus_tag="KPD58_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0267286.1"
                     /translation="TLFVGLALVLAIGITACTKNNEKPSENTGKNPNESVNNNEGYMN
                     YYSTSYNDYIAGLNRYSIYDTPESINNQFKDKEYPGNEKYLSDVKAAYKDSRDKIQSF
                     VTSLKKDGKTEDTELKKMNDDLIAEGERLVKDIDKRIEKLDKVSDEDMKKGQNDFIKL
                     VHGTEDVGNDIGSGFRKMIKDMNDRLGITGNNKK"
     gene            complement(756..1223)
                     /locus_tag="KPD58_003564"
     CDS             complement(756..1223)
                     /locus_tag="KPD58_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0267287.1"
                     /translation="MENDTIKADDILNYCLSNLDDVVLMDSWGERAIYYNPNGVLKRG
                     VYVLTIKEKDSNNDKGSLVSRPNVYRVNIGLKKETFIEMFGYIPKRPGVGQIVDMDFD
                     FTKLDTIMPHPIYSWMGWICVLSPTEKTFENFKTLIGESYNFAKQKFKKRKNK"
     gene            1334..2278
                     /locus_tag="KPD58_003565"
     CDS             1334..2278
                     /locus_tag="KPD58_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HBG0267288.1"
                     /translation="MSRAERLIELMITINAKRSFTAGELAEEFSVSKRTILRDLQVLE
                     SIGFPLYSKVGAAGGYHVLKERILPPIAFSESEVKSIFFAYQSLEYYNDLPFEQETIS
                     VLKKFLNCIPNDIQYNIENIRRKFVFWTPDRHCSTPLLKELFNIVMNEFTIKIEYSSK
                     QKNSVRTIVPIGLYAMNGLWYCPAYCIKSESIKEFRVDRIVKILSIENLSSKKYKVLS
                     SIHDYLKNMEVGTDYHIKINLTDEGVKRCETEFLLARGLKILSKGGYIDMYIPKSTLN
                     WVAEYFLTFGKNATIIEPIELKHLIKSKVLELYNHHCI"
BASE COUNT          901 a          277 c          378 g          829 t
ORIGIN      
        1 aactttattt gtaggattag cactagtatt agctataggt ataactgcat gtactaaaaa
       61 taatgaaaaa ccaagtgaaa atacaggaaa aaatcctaat gaatctgtaa ataataatga
      121 aggatatatg aattattatt caacaagcta taatgattat atagcaggtc taaatagata
      181 ttctatttat gatactccag aaagtataaa taatcagttt aaagataaag aatatccagg
      241 aaatgaaaaa tatctaagtg atgtaaaggc tgcatataaa gatagtagag ataaaataca
      301 atcatttgta acatctctta aaaaagatgg taaaacagaa gatacagagt taaagaaaat
      361 gaatgatgac ctaatcgctg aaggggaaag attagtaaaa gatatagata agagaataga
      421 aaaattagat aaggtatctg atgaagatat gaaaaaaggt caaaatgact ttataaaatt
      481 agttcatgga actgaagatg ttggaaatga tataggtagt ggttttagaa aaatgataaa
      541 agatatgaat gatagattag gaataacagg aaataataag aaataataga ataaatagtt
      601 ttgaaatttg aaataaatta tttgagatat agcgaagcct tataaataat acattttttg
      661 gcttcgcttt tttgcatgtt attaagatat taatttttat cttgtaacct taatattttt
      721 attataattt atttagaggt tattttcaca cgtttttact tgttttttct ctttttaaat
      781 ttttgttttg caaaattgta agattctcct attaaagttt taaagttctc aaatgtcttt
      841 tcagtagggc ttaggacaca tatccatccc atccatgagt agataggatg tggcatgatt
      901 gtgtccaatt ttgtaaaatc aaaatccata tcaactattt gacctacacc tggacgcttt
      961 ggaatatatc caaacatttc aataaaagtt tcttttttta atcctatatt cacacggtat
     1021 acatttggac gactaactaa cgaaccttta tcattattac tgtctttttc cttaatggta
     1081 agaacatata cccctcgctt taaaacacca ttaggattgt agtaaattgc tcgttccccc
     1141 caactatcca ttagtacaac atcatctaag tttgatagac aataattgag aatatcatca
     1201 gccttaatag tatcattttc cataaagtat caatcccttc tttctttact attattattg
     1261 ctttagatgc aagaatatct tatcatataa tagtgacaat tattgtcact atatttaaat
     1321 taggagtata aatatgtcaa gagctgagcg tttaattgaa ctgatgataa caataaatgc
     1381 aaaaagaagt tttacagcag gagaattagc agaagaattt tctgtatcaa aacgtactat
     1441 actaagagat ttacaggtgc tggagagtat tggatttcca ttatattcaa aagttggagc
     1501 ggctggtgga tatcatgtac ttaaagagcg tatacttcca ccgattgcat tttcagaaag
     1561 tgaggtaaaa tctatattct ttgcatatca gtcactagaa tattataatg atttgccatt
     1621 tgagcaagaa actatttctg ttttaaaaaa atttttaaat tgtataccaa atgatattca
     1681 atataatatt gagaatataa ggcgtaaatt tgttttttgg acaccagata ggcactgttc
     1741 aacaccatta ttgaaagaat tgtttaatat agtaatgaat gaatttacaa ttaaaattga
     1801 atattcatca aagcaaaaaa atagtgtacg taccattgtt cctattggtt tgtatgccat
     1861 gaatggactt tggtattgtc cagcttactg tataaaatca gaatctatta aggaatttcg
     1921 tgtagacaga attgtgaaga ttttgagtat agaaaactta tcaagtaaga aatataaggt
     1981 tttatcttct atacatgatt atcttaagaa tatggaagta ggtacagatt atcatattaa
     2041 aattaatttg acagatgagg gagtaaaacg ttgtgaaaca gaatttttgt tggcaagagg
     2101 gttgaaaatc ttgtctaaag gtggatacat agatatgtat ataccaaaat ctacacttaa
     2161 ctgggtggct gaatattttt taacttttgg gaaaaatgct acaataatag aacctataga
     2221 acttaaacat ttgataaagt ctaaagtact agagctatat aatcatcatt gtatttaaat
     2281 tattttattt aatagtagga tagagtttgg ataaaatgtt aattttctta atttacttta
     2341 cagatgattt agatagattg atgttattta atttatttct tataa
//
LOCUS       DAELLV010000350         2367 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.351, whole genome shotgun sequence.
ACCESSION   DAELLV010000350 DAELLV010000000
VERSION     DAELLV010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.351"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            259..1560
                     /locus_tag="KPD58_003566"
     CDS             259..1560
                     /locus_tag="KPD58_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0267289.1"
                     /translation="MGKSKDVEIEKSILGTILLDNKLSYKLDELNENMFMNDICLEIF
                     KIMKELKKENIVIDVATVKSKIDRKSLAIKTSDVTNLITWGQNFGLDGHIKILKENLA
                     RRSINQNCQNLLHSLNLGENIDTCIYKFESNIKEILDKDTYENDDVNSIAGKVLDFLE
                     NKKDIGFKFGIKLLDTTIGGLFKGELTTIAAKSGVGKTALALQIMLNSFKQGKKTLFI
                     SREMTSEQVFMRNICRVTGVSTRNMKSKEIDENDWKLIVNAIGDLSENNLIYINDKID
                     TISAIRKRIRQVKPDLLIVDYVQLLTSQKSMDKREREVATFSRELKNMTLDFNIPVIQ
                     LSQLNDEMKDSRPYGDRPMRDSKAIYHDSNNVVYIHQLKGSDYEEAVRDIGESEEAVR
                     ASEYRGIKMVDLIVAKCRDGQTRHKHFCYFGDKLHFQELNY"
     gene            1636..1911
                     /locus_tag="KPD58_003567"
     CDS             1636..1911
                     /locus_tag="KPD58_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG0267290.1"
                     /translation="MLELRKIIELLEEHKIEVSIPKELHKYSLLEYLELCQQSGGTYF
                     YIKTLEGALKEARNELMIKLFNGKNYMELARMFEMSVINVRHIVRKK"
BASE COUNT          974 a          192 c          463 g          738 t
ORIGIN      
        1 tactatcgag taatgaatta tattgaacaa tatattaata attggcagta agttatatca
       61 aataagatat tgctattatg tagtaaatta tataaaacaa tgtatcagaa taaatagtat
      121 attaataatt tgtagtatat catgacgaat aatacagtca taacaaatag tgagtcataa
      181 caaataatgt atttataatt gataatgtat ttaaaaatca aaatataaaa acttataagg
      241 aaagagagat taatttatat gggtaaatca aaagatgttg aaatagagaa gtctatttta
      301 ggcacaattc ttttagacaa taaattaagc tataagctag atgaattaaa tgaaaacatg
      361 tttatgaatg atatatgtct tgagatattt aaaattatga aagaacttaa aaaagaaaac
      421 atagtgattg atgtggccac tgtgaaatca aagatagata gaaaatctct agcaataaaa
      481 acgagtgatg tgactaatct aattacttgg ggtcagaatt ttggtcttga tggtcatatt
      541 aaaattttaa aagaaaattt agctagaaga agcataaatc aaaactgcca aaacctttta
      601 cacagcctaa accttggaga aaatatcgat acttgtattt acaaatttga aagtaatata
      661 aaggagattt tggacaagga tacttatgaa aatgatgatg taaatagtat agcagggaag
      721 gtgcttgatt ttttagaaaa caaaaaagat attggattta aatttggtat aaaattactt
      781 gataccacta taggaggtct ttttaaagga gagctaacca caatagcagc taaatcgggg
      841 gttgggaaga ctgctttggc acttcaaata atgttaaatt catttaagca aggtaaaaaa
      901 actttattta taagtagaga aatgaccagt gagcaagtat ttatgagaaa tatatgcaga
      961 gtaactggtg taagtacaag gaatatgaaa tctaaagaaa tagatgaaaa tgattggaaa
     1021 cttattgtaa atgctatagg tgatttaagc gaaaataact taatttatat caatgataag
     1081 atagatacaa ttagtgcaat tagaaagagg ataaggcagg taaagccaga tttattaata
     1141 gtggattatg tgcaactcct tacatctcag aaaagcatgg ataagagaga aagggaagta
     1201 gctacttttt cgagagaact taaaaatatg actttagatt ttaatatacc tgtaatccaa
     1261 ttaagtcagt taaatgatga aatgaaagat tcaagacctt atggagatag acctatgagg
     1321 gatagcaaag ccatctacca cgatagcaac aatgtggttt atatacatca attaaagggg
     1381 agtgattatg aagaagcagt gagagatatt ggagaaagtg aggaggcagt tagagcatca
     1441 gaatacaggg gaattaagat ggttgattta atagttgcga agtgtagaga tggacagact
     1501 aggcataagc atttttgtta ctttggggat aagttacatt ttcaagaact aaattattag
     1561 gtcaataaag tagtaataac aagcttatta aaagtcggga aatagcaaat agattataaa
     1621 atatgaggtg aaaatatgtt ggaattaaga aaaattatag aattacttga agaacacaag
     1681 atagaagtaa gtattccaaa ggagttacat aagtatagtt tattggaata ccttgaattg
     1741 tgtcaacaaa gtgggggaac atatttttat attaaaacac tggaaggggc tttaaaagag
     1801 gctagaaatg agcttatgat taaattgttt aatgggaaaa attatatgga attagcgagg
     1861 atgtttgaaa tgagtgttat taatgtcagg catattgtta gaaaaaaata gatatttttg
     1921 gagaatacaa gtgtgatata cttctgaaat agaagttatt atagttgaaa taaaattgtg
     1981 gttaataaat taaagttgtg tataagttta agattataga ggagtataag tgtgattaac
     2041 aacatgattt tgtgaataaa attgaggata tatatgagtt aaatgtgtga ttaataaggt
     2101 aaggttgtgt ataagattat gagtatttaa aaggaggggc tataattaat agtgtagagt
     2161 tgtgtgtttg cttaagatta tataataatt taatagatta aactatacta ataaggtaaa
     2221 atcgtgtgtt tagttaaagc ttattataaa atcttgatat gtgattaatg atgcaatatg
     2281 tgaataaagt taagattata atagatttaa cctgtggcta atagaataaa attgtgaata
     2341 aagttaagat tataataggt ttaacct
//
LOCUS       DAELLV010000351         2351 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.352, whole genome shotgun sequence.
ACCESSION   DAELLV010000351 DAELLV010000000
VERSION     DAELLV010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.352"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            40..228
                     /locus_tag="KPD58_003568"
     CDS             40..228
                     /locus_tag="KPD58_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0267291.1"
                     /translation="MTKTILCDYCNKGINKDDNKYITFHKKSHMKTNICINCALNLID
                     KDKLNENIINNQHDYSKK"
     gene            complement(476..1180)
                     /locus_tag="KPD58_003569"
     CDS             complement(476..1180)
                     /locus_tag="KPD58_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0267292.1"
                     /translation="MRLKFKSKESTSREKHQSAESLAKFMLNNKSFLGITLSILDEWK
                     NNIDSSKIEDIVKNNFRFEESIIKDKNYKEFIKLLNSFYNGTEDELNGRRGRFLELIW
                     DVVGTYNGKEFTQKIDEAIVLENEVKISEKDIDIVYIGEIKDKGIANFIEMHECKASA
                     NTTLRTPLKPKHRGKLELMQSVIEISETEEIKCDGLIITFNANKRRLERKLKSYGFEY
                     FKIIPRAEIEERVFNK"
     gene            complement(1182..1613)
                     /locus_tag="KPD58_003570"
     CDS             complement(1182..1613)
                     /locus_tag="KPD58_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HBG0267293.1"
                     /translation="MELDKMKYRKLVNDVELVNVLIKNIELSDVILHKDENIKNESIK
                     CDVNMKYECEEFKKLSEVVEFYPKFNLRIECQGEGLVNLKFELRAIYKFNSIEDYEDD
                     YILMFIERNVPVNIWPYAREIISSITTRIGYPALVISPYKA"
     gene            complement(1631..2194)
                     /locus_tag="KPD58_003571"
     CDS             complement(1631..2194)
                     /locus_tag="KPD58_003571"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0267294.1"
                     /translation="MNNYSIMFENKSILDLKQTCYLDSLYNLFEEKIQYVTKTLKNNN
                     IEEDEWDIQFIKEDALQSIKNIEEDFITDKTLDWRINLSKIIKKVLDKSKANFKIADE
                     MYHNPMKKSEQYYDLLSNIFDNLEELICIKYKEYEISRNIRFTNIGNLVNNDNSSNYI
                     DNEHIEFDFNFKFEIEDYDNDDYALAA"
BASE COUNT          785 a          356 c          215 g          995 t
ORIGIN      
        1 acttaattat ggaaaatata aaatgaaagg aataaaaata tgactaagac tatattatgt
       61 gattactgta ataaaggaat aaacaaagat gataataagt atattacttt tcataagaaa
      121 agtcatatga aaactaacat ttgtattaat tgtgcattaa atttgataga taaagataaa
      181 ttaaatgaaa atattataaa taatcaacat gattattcaa agaaatgaaa aaagcactct
      241 ccataatgaa gaatgctcta tataataatg tttgacttag taaagatgtg ttggggttac
      301 atatttactt ttttattata tcatataact ttgtgtatga aaaagaattt aaatcgattt
      361 taaagtgtgt cgagtaaggt tcttgatagt ttatatgtta ataaatttca aaaaaatagc
      421 actcttacaa aaagagtact tttagtatct attaatttgt ataaattagt gtaaattact
      481 tattaaacac tctttcctct atttctgctc taggaattat tttaaagtat tcaaatccat
      541 agctttttag ctttctttca agccttcttt tgttagcatt gaatgtaatt attaatccat
      601 cacatttaat ttcttctgtt tctgatattt caattactga ttgcatcagt tctagttttc
      661 ctctatgttt tggttttaat ggagttctta gtgttgtgtt tgctgatgct ttacattcgt
      721 gcatttcaat aaaatttgct atccctttat ctttaatttc gcctatatag acaatatcta
      781 tatctttttc tgatattttc acttcatttt ctaacactat ggcctcatct attttttgtg
      841 taaattcttt tccgttatat gttcctacaa catcccaaat caactctaaa aatcttcctc
      901 ttctaccgtt taactcatct tctgttccgt tataaaatga gtttaataat tttataaact
      961 ctttatagtt cttatcttta attatagatt cctcaaatct aaaattattt tttacaatat
     1021 cctcaatttt actactatca atattatttt tccattcatc taatattgac agtgttatac
     1081 ctaagaacga tttattgttt aacataaact ttgctagaga ttctgctgac tgatgttttt
     1141 ccctgctagt actctcttta gatttaaatt tcaatctcat attatgcctt atatggagaa
     1201 attactagag caggatatcc aatacgagtt gtgatggaac ttattatctc tcttgcataa
     1261 ggccatatat taactggaac atttcgttct ataaacatta atatataatc atcttcataa
     1321 tcctctattg aattaaattt ataaattgcc cttagttcaa attttaaatt taccaaacct
     1381 tctccttgac attctattct taagttaaat ttaggataaa attctactac ttctgataat
     1441 tttttaaatt cttcacactc gtatttcata ttaacatcac atttgatact ttcatttttg
     1501 atattttcat ctttatgtaa tataacatcg cttaattcaa tatttttaat tagcacattt
     1561 actagttcca catcattaac taattttcta tacttcattt tatctaattc cattaatctt
     1621 cctccaaaat ctaagctgcc aatgcataat catcattatc ataatcctct atttcaaact
     1681 taaagttaaa atcaaattca atatgttcat tgtctatata atttgatgag ttatcattgt
     1741 taactaaatt tccaatatta gtaaacctga tatttctaga aatttcgtat tctttatatt
     1801 ttatacatat aagctcttct aaattatcaa agatattact taataaatca taatactgtt
     1861 cacttttctt cataggatta tgatacattt catctgctat tttaaagtta gccttgcttt
     1921 tatctaatac tttctttatt atttttgata aattgattct ccaatctaat gttttatcag
     1981 taataaaatc ttcttctata tttttaattg actgcaaggc atcttctttt ataaactgta
     2041 tatcccattc atcctcttct atattgttat tttttagagt tttagttaca tactgaattt
     2101 tttcttcaaa taaattatat aagctatcta aatagcatgt ttgttttaaa tctaaaatag
     2161 acttattttc aaacataatt gaataattat tcatattaaa ttcccccttg taaacaaatc
     2221 tttccataaa tatacctcca agagtataat ttaagatttg ttacctaaat tataccattt
     2281 taatatttac attccaacaa caaatattat taatattttc tcatatatac taagttttta
     2341 tacatctgct c
//
LOCUS       DAELLV010000352         2323 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.354, whole genome shotgun sequence.
ACCESSION   DAELLV010000352 DAELLV010000000
VERSION     DAELLV010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.354"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            402..2297
                     /locus_tag="KPD58_003572"
     CDS             402..2297
                     /locus_tag="KPD58_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp8"
                     /protein_id="HBG0267295.1"
                     /translation="MLSNKKRSMAIVMAGATVMSAAAPIFADNTVTENVDKNYTVSAK
                     DSAKLIEEVRKALEVKFEDTKAGANVNDRVYDIKVDNVNLTNATQLQNKINSLTEGQS
                     LKVTIQDKGHQVLGGKVVDYKIENYKTAQEIVDAVNAYNATLAEDSDNKLTATIKSTN
                     TVEVKRAKDSANVITLNVGDQHLDFSKVITSEEGTFEGYEKSYSDIDSKELHTITVKN
                     ADLQDISAEELFDGIRLTTLGREIVNKVKNGYALTFENEAILTQEQEDSDDKDKPEKS
                     SFDIVLSKANEKPETISVSSKNHKLVRDLHKVLTDVKDGKELKVEVLSGDSRFTTAVE
                     VSKERFKDGEAEAIILVGEDAIVDGLASAPLASQKNAPILLSKKDSLPSEIEAEILRV
                     LGSNLSSKKIYIVGGESKVSKETEEKLSKLGVSKVERVSGEDRFETSLEIAKQLKDTF
                     KTAFVVGGNGEADAMSISARAAQFGAPIIVTGNELDANAEKLLKGKELEIVGGENSVS
                     KEVEDKLVDIDLNNKVERLAGENRKDTNAKVINKYYAGATKAYVAKDGYVGGNGQLVD
                     ALTAAPLAASSKAPIVLTTEELSKSQEEVVELRLKNATKLVQIGEGIAKNAIEKIAEK
                     INLFTKN"
BASE COUNT         1011 a          235 c          429 g          648 t
ORIGIN      
        1 ttagaataat ttttaatttt aaactctaat ttatgatgat ttttataata tgttatatat
       61 tttaatatat ggagatagat attaatttag tgatatatat ttaagtaata agagtaaagc
      121 aaaaattagt gcatagtagt atataaaaat ggagtttgaa tacttttgtt agtatatgat
      181 tgtttagtag aaaatcacta taaaatagga tttctatctt tgagcataat tatagagagc
      241 ctaagtaggg ggaagggttt caagaagata ttattagtgt ttgtgttagt aaattatgta
      301 aacacataaa atatattgaa aaataatatt atatataata ttatttatgt gtgggaaata
      361 cgaatataaa aaataaaaaa taatattcgg aggacataaa tatgttaagc aacaaaaaga
      421 gaagtatggc aatagtaatg gcaggtgcta cagttatgag tgcagcagca ccaatattcg
      481 cagacaatac cgtgacagaa aatgttgata aaaattacac agtaagtgcg aaagattctg
      541 ctaaactaat cgaagaggtt agaaaagcat tagaagttaa gtttgaagat acaaaagcag
      601 gagcaaatgt aaatgataga gtttacgata taaaagtaga caatgtaaac ttaactaatg
      661 caactcaatt acaaaataaa ataaactctt taacagaagg gcaaagttta aaagttacta
      721 ttcaagataa agggcatcaa gtgttaggtg gaaaagtagt tgactataaa attgaaaact
      781 acaaaactgc tcaagaaata gtagatgcag ttaatgctta caatgcaact ttagcagaag
      841 attctgataa caagttaaca gcaactataa aatctacaaa tacagttgaa gtaaaaagag
      901 caaaagattc agcaaatgtt attacattaa atgtaggaga ccaacattta gatttctcaa
      961 aagtgataac atcagaagaa ggtacttttg aaggttatga aaagagttat agtgacatag
     1021 attcaaaaga attacatacg ataacagtta aaaacgcaga tttacaagac atatcagcag
     1081 aagaattatt tgatggaatt agattaacta cattaggaag agaaatagtt aataaagtta
     1141 aaaatggata tgctttaaca tttgaaaatg aagcaatcct tactcaagaa caagaagata
     1201 gtgatgataa agataaacca gaaaaatcaa gctttgatat agttttaagc aaggcaaatg
     1261 aaaagccaga aacaataagt gtttcaagta aaaaccataa gttagttaga gacttacaca
     1321 aagtattaac tgatgtaaaa gatggaaaag aattaaaagt agaagtttta tctggtgatt
     1381 caagatttac aacagcagta gaagttagta aagagagatt taaagatgga gaagcagaag
     1441 ctataatttt agttggagaa gatgctatag ttgatggatt agcatcagca ccacttgcct
     1501 ctcaaaaaaa tgcaccaata ttattatcta aaaaagattc actaccatca gaaatagaag
     1561 ctgaaatatt aagagtactt ggaagtaatc tatcttctaa gaaaatatat atagtaggtg
     1621 gagaatctaa agtatcaaaa gaaactgaag aaaaactttc taaattaggt gtaagtaaag
     1681 ttgagagagt ttctggagaa gatagatttg aaacttcttt agaaatagca aaacaattaa
     1741 aagatacatt taagactgct tttgtagtag gtggaaatgg agaagctgat gctatgagta
     1801 tatcagctag agctgctcaa tttggtgctc caataatagt tacaggtaac gaattagatg
     1861 caaatgctga aaaattatta aaaggaaaag aattagaaat agtgggtgga gaaaattctg
     1921 tatcaaaaga agttgaagac aaattagttg atatagattt aaataataaa gttgaaagat
     1981 tagctggaga aaatagaaaa gatactaatg ctaaagtaat caataaatac tatgcaggtg
     2041 caactaaagc atatgtagca aaagatggtt atgtaggtgg aaatggacaa ttagttgatg
     2101 cacttacagc agcaccactt gcagctagtt caaaggctcc aatagtatta actacagaag
     2161 aactttctaa atcacaagaa gaagtagttg agttaagact taagaatgct actaagttag
     2221 tacaaatagg tgaaggaata gctaaaaacg ctattgaaaa aatagcagaa aaaataaact
     2281 tatttactaa gaactaataa gatataggaa ttaatattaa aag
//
LOCUS       DAELLV010000353         2287 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.355, whole genome shotgun sequence.
ACCESSION   DAELLV010000353 DAELLV010000000
VERSION     DAELLV010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.355"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            80..1888
                     /gene="dptF"
                     /locus_tag="KPD58_003573"
     CDS             80..1888
                     /gene="dptF"
                     /locus_tag="KPD58_003573"
                     /inference="COORDINATES: protein motif:HMM:TIGR03238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA phosphorothioation-dependent restriction
                     protein DptF"
                     /protein_id="HBG0267296.1"
                     /translation="MSNGCLIDELKKLKESSKEAVENISDFSSFKKYMHVKRQVENEL
                     LDIIKYTADSNKAELILVCGGVGDGKSHLISYLNNKYPEIMSKFIIHNDATESFEPNK
                     TSIDTLNDVLDDFSDKKMDNSDKKLVLAINLGALSNFLESEYVNKFTKLREFVENKGI
                     LDISISDDDDKNSNFHFVNFSDYHIYTLNADKPKSRYIESIINKIIAEDNQNIFINSY
                     KTNCTHCIVNYKCPIKNNYEMLMRDSVRETLVDILIESTVKYKIIISTRSLLNFIYDF
                     IVNVDLDNKNNKDLIKFVEELSYEAFIKYIFVSNLYNHSELSQILNAVNKIDPINITS
                     ENLDNIIIKLSNTKDMTILFNQYLNLSKNSYLESSMTNREIIESALNSSKNELSRKIL
                     KYNIINTFIRLHIFIPKNNLQLRDNIYISYMRNLYYFNKGEKTYLSNLYDDIKEALYS
                     WNGRIQDDRINMSIGKNQMRYKISQRLNIIPDLSKLRKCTEEELHKFKPSLEIGYKND
                     EKENQDTCTVDIDFNLYKLLMRVKYGYTPNNNDKYTYINFVVFMNKIHKLGNQNRELY
                     IEDRLEDTVPKYKLTYDKEFDKFKFIRVNGVKVCNL"
     gene            1876..>2287
                     /locus_tag="KPD58_003574"
     CDS             1876..>2287
                     /locus_tag="KPD58_003574"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0267297.1"
                     /translation="MQLIINKEYFKQNYMIDKGIARHNSVKNIVILPYTTTTKEKSKL
                     KEKVERFESCVGEFCRLVYNKKMNIKERQLKDIKLLIKDINVNSSDDSVQLESIIREL
                     FLDEEENMYIFHPKLLNYIHMPSDSYYKKLSKFLY"
BASE COUNT         1020 a          194 c          337 g          736 t
ORIGIN      
        1 aaagggatct caaagaaata aatagcattt ataatataag tgtagtataa aagagtatat
       61 tagaaggagt tttaaaatta tgtcaaatgg ttgtttgata gatgaattaa agaaattaaa
      121 agaatcatca aaggaagcag tagaaaatat ttcagatttt agtagtttta aaaaatatat
      181 gcatgttaaa agacaagtag aaaacgaatt acttgatatt ataaaatata cagctgattc
      241 taataaagct gagttgatat tggtatgtgg tggagtagga gatggaaaat cgcatttaat
      301 atcatattta aataacaaat atccagaaat tatgtcaaaa tttataattc ataatgatgc
      361 tacagaaagt tttgaaccaa ataaaacatc aatagataca cttaatgatg tacttgatga
      421 tttttcagat aagaaaatgg ataattcaga taaaaagtta gtattagcaa taaatttagg
      481 ggcacttagt aattttcttg aatcagaata tgttaataaa tttactaaat taagagagtt
      541 tgttgaaaat aaaggaatac ttgatatatc tatatcagat gatgatgata aaaattcgaa
      601 ttttcatttt gttaatttta gtgattatca tatatatact ttgaatgcag ataaacctaa
      661 atctagatat atagaatcta taattaataa aataatagca gaagataatc aaaatatatt
      721 tataaatagt tataaaacaa attgtacaca ttgtattgtc aattataaat gtccgattaa
      781 aaataattat gaaatgttaa tgagagactc agtgagagaa accttggtgg atatattgat
      841 agaatctaca gttaaatata aaattataat ttcaacaagg tctcttttga attttatata
      901 tgattttatt gttaatgttg atttagacaa taaaaataat aaagacttaa ttaagtttgt
      961 agaagaacta agttatgaag cattcataaa atatatattt gtatctaatt tatataatca
     1021 tagtgaatta tctcagattt taaatgcagt aaataaaatt gaccctataa atatcacatc
     1081 agaaaaccta gataatataa tcattaagct tagtaataca aaagatatga caatactatt
     1141 taatcaatac ctgaatttaa gtaaaaacag ttatctagaa agttctatga caaatagaga
     1201 aataatagag tcggctttga attcttcaaa gaacgaactg agtaggaaaa tattaaaata
     1261 caatataata aatacattta taagactaca tatatttata cctaaaaata atttacagtt
     1321 aagagataat atatatataa gttacatgag aaatttatac tattttaaca aaggagaaaa
     1381 aacttattta agtaatttat atgatgatat taaagaagct ttgtatagtt ggaatggacg
     1441 tattcaagat gacagaataa atatgtccat tggaaaaaat cagatgagat ataaaataag
     1501 tcaaaggcta aatataattc cagatttaag caaattaaga aaatgtactg aggaagaatt
     1561 gcacaagttt aagcctagtt tagagatagg gtataaaaat gatgaaaaag aaaatcaaga
     1621 tacttgtaca gtggatatag attttaattt atataagtta ctaatgagag taaaatatgg
     1681 atatacacca aacaataatg ataaatatac ttacataaat tttgtagtat ttatgaataa
     1741 gatacataaa ttaggaaatc aaaacagaga actatatata gaggatagat tagaagatac
     1801 tgtgcctaaa tataaattaa catatgataa agaatttgat aaattcaaat ttataagagt
     1861 aaatggggtg aaggtatgca acttataata aataaagaat actttaaaca aaattatatg
     1921 atagataaag gtatcgcgag acataatagt gtcaaaaaca tagttatact cccatataca
     1981 actacaacta aagaaaaatc gaaacttaaa gaaaaggtag aaagatttga gtcttgtgtt
     2041 ggagaattct gtaggttggt atataacaag aaaatgaaca taaaagaaag acagctaaaa
     2101 gatataaagt tacttataaa agacataaat gtgaattctt ctgatgatag tgtacaactt
     2161 gagagtataa taagagaatt atttttggat gaagaagaaa atatgtatat atttcatcca
     2221 aagcttttaa attatataca tatgccatct gatagttatt ataaaaaatt gtctaaattt
     2281 ttatatg
//
LOCUS       DAELLV010000354         2267 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.356, whole genome shotgun sequence.
ACCESSION   DAELLV010000354 DAELLV010000000
VERSION     DAELLV010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.356"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            120..2192
                     /locus_tag="KPD58_003575"
     CDS             120..2192
                     /locus_tag="KPD58_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG0267298.1"
                     /translation="MKKVFGVLQKVGKSLMLPVALLPAAGILLGVSNALAGDALLAHF
                     PALANPTFQLIISIMESSGQIIFNNLPLIFAVGVAVGLTEGEGVAALAAIVGFLILNT
                     SMGVMAGVTEDMVDNSMYANIVGIPTIQTGVFGGIIIGIVAAYLYKKFYKIELPQYLG
                     FFAGKRFVPIVTSLVAIAIGIILSFIWPPIQEGLLSFSKNVIEANQTLAAFMEGSLER
                     LLIPFGLHHVFHNPFWYQFGEYVNKAGELVMGDQKIFFAQLKDGVNFTAGTFMTGAFP
                     FMMFGLPAAALAMVHEAKPEKKKFVAGIMASAALTSFLTGITEPIEFAFLFVAPVLFA
                     IHCLFSGLSYAIMQLLGVKIGFTFSGGLIDFLLFGVLPNRTPWYLAILVGIGFAVLYY
                     SVFRFMIRKFNLKTPGREDESETNAEVIEMSKNELASEILIALGNKDNLKSLDACITR
                     LRLVVNDINKVDKEKLKSLGAAGVMVIGNNVQAIFGPKSDSIKSDIKDIIEGKTKVES
                     TVTKNVDKNKVKKNMEVANMENALVEKIDSLTNGEILDIEEVPDSVFSSKLMGDGFAI
                     KSSDGLIYSPVDGTIGVIFPTKHAIIIKSQKSGVEILIHLGIETVNLEGNGFEVFVNV
                     GDEVKAGEKLVKMDLEYIEKNGLSTISPVVFTNLEQNQKLSIKKGVTTAKEENRVSIL
                     RDDIEKIG"
BASE COUNT          829 a          252 c          444 g          742 t
ORIGIN      
        1 ataggtttat ttatattaac ataagtttaa ttttagaact aagtatacta atgtaacttt
       61 ttataaactt aagtcttttt tatttaaaaa aatataaaat aataaggaga gaaaagtaaa
      121 tgaaaaaagt atttggtgtt ttacaaaaag taggaaaatc tctaatgcta ccagttgcat
      181 tacttcctgc agcaggtata ttacttggcg tgagtaatgc tttagcagga gacgcattgt
      241 tggctcattt tccagctttg gcgaatccta catttcaact tataataagt ataatggaaa
      301 gttcaggtca aattatattt aataacttac cacttatatt tgcagttggg gtagctgttg
      361 gtttaactga aggagaaggt gttgcagcat tggcagccat agtaggtttt ttaattctca
      421 atacatctat gggtgttatg gcaggtgtta cagaagatat ggtagacaat tctatgtatg
      481 caaatattgt tggaatacca acaattcaaa caggggtatt tggtggtatt attataggga
      541 tagtggctgc ttatctttat aaaaagtttt ataaaattga attacctcag tatctaggtt
      601 tttttgcagg taaaagattt gtaccaatcg ttacttcatt agttgctata gcaataggaa
      661 ttatattgtc ttttatttgg cctccaatac aagagggatt acttagtttc tcaaaaaatg
      721 ttatagaagc aaatcaaaca ctagcagcat ttatggaagg ttctttggaa agactattaa
      781 taccatttgg tttgcatcat gttttccata atccattttg gtatcaattt ggagaatatg
      841 taaataaagc tggtgagctt gtaatgggtg accaaaagat attctttgct caacttaaag
      901 atggagtaaa tttcacagca ggtactttta tgacaggtgc atttccattt atgatgtttg
      961 gtcttccagc agcagctctt gctatggtgc atgaagcaaa gccagaaaag aaaaaatttg
     1021 tagcaggtat aatggcatca gcagcattaa cctctttttt aacaggaata accgagccaa
     1081 tagagtttgc gtttttattt gttgcaccag tattatttgc aatacattgt ttattttcag
     1141 gtttatcata cgcaattatg caattattag gagttaaaat tggattcaca ttttcaggag
     1201 gattaataga tttcttatta tttggtgttc ttcctaatag aactccatgg tacttggcta
     1261 tcttggtagg tattggtttt gcagtattat actatagtgt atttagattt atgataagaa
     1321 agtttaattt aaagacacca ggaagagaag atgagtcaga aactaacgct gaagtcattg
     1381 aaatgtctaa gaatgaatta gcatcagaga ttttgattgc tttaggaaat aaagacaatt
     1441 taaagagcct agatgcgtgt ataacaagac ttagattggt tgtaaatgat ataaataaag
     1501 tagataaaga aaaattgaaa tcacttggag cagcaggtgt tatggttatt ggcaacaatg
     1561 ttcaagctat atttggacca aaatcggatt caataaagtc tgatataaaa gatataatag
     1621 agggaaaaac aaaggttgaa agtactgtta ctaaaaatgt tgataaaaat aaagtgaaaa
     1681 aaaatatgga agttgcaaat atggaaaatg ctttagtaga aaagattgat tcattaacaa
     1741 atggagagat attagatata gaagaagtgc cagattctgt attttcaagt aaactgatgg
     1801 gggatggatt tgcaattaaa tctagtgatg gattgattta ttcaccagtt gatggaacta
     1861 ttggagttat atttccaact aagcatgcaa taataataaa atcccaaaaa agtggagtag
     1921 aaatacttat acatttaggg atagaaactg taaacttaga aggaaatgga tttgaagtat
     1981 ttgtgaatgt aggagatgaa gtaaaagcgg gagaaaaatt agttaaaatg gacttagaat
     2041 atattgaaaa aaatggtcta tcaacaataa gtcctgtagt atttacgaat ttagaacaaa
     2101 atcagaaatt aagcattaaa aaaggagtaa ccacagcaaa agaggagaat agagtaagta
     2161 tattaagaga tgatattgaa aaaataggat agctatatga tagtgtaact aaaagttatg
     2221 tgagatttaa taaatatata aataggttca gttaacatgc aatttat
//
LOCUS       DAELLV010000355         2243 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.357, whole genome shotgun sequence.
ACCESSION   DAELLV010000355 DAELLV010000000
VERSION     DAELLV010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.357"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            170..1036
                     /locus_tag="KPD58_003576"
     CDS             170..1036
                     /locus_tag="KPD58_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0267299.1"
                     /translation="MELSVERLTKQYNNKIIVDKIDFKLNEGIYGFLGENGAGKTTLM
                     RMICGILSPTSGEVKVDGKNNLDMGEDFRDMIGYLPQNFGYYPDFTAKEFMLYIASLK
                     GLMPSYAKRKTQELLEMVGLSEVINKKIRTFSGGMKQRLGFAQTLLNDPKILILDEPT
                     AGLDPKERVKFRNLISEFSKEKIVILSTHIVSDVEYIADKILIMKKGRFVLTGSVEEV
                     TSKIKDKVWKCVVNENEIDYMSDRYCLANIHHSGGVANLRIVSDIKPHDNACNLKPTL
                     EDLYLYCFNEVL"
     gene            1057..>2243
                     /locus_tag="KPD58_003577"
     CDS             1057..>2243
                     /locus_tag="KPD58_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBG0267300.1"
                     /translation="MSTLIKFELKKIIKRKSTIAVILGSLFLSIILFALMPIQEKAND
                     ESGKEYRGLQAIELKKQQSKLNEGYITDKKVKNSIRDYQKMFNNPNNIVTNESGFSDF
                     KPDIYWKFFYPNKTFYNFIIESYSKPNDFNPEVIKSMPLENEINFYKFRNEKVNTILN
                     MEYPEGNYTQKEKDFWLSKNKDIKEPYLNGYYQGWEYFLLARDLLIFTIMAVCISIAS
                     VFSGEYQSGADSVVLTSKYGKTKVITAKIISSFLFGTIIFTINTIVSIGILLLSFGTD
                     GWNLPLQLTTSSIPYPYTFLQAILLSVLTIYMVLIVLISFTLLLSSYMKSPFPVLITN
                     IILLFVPIFFKYSETNKLYNQILNVLPSNAISFSFTNYVDYKFGNLIIALPTMIIFTC
                     LVLT"
BASE COUNT          888 a          259 c          354 g          742 t
ORIGIN      
        1 agagataatc tatatcaatt tgaagcaata aatcaatggc taattgtttt tctagtcaca
       61 gcagtaacat taatatatga aataagaaaa acgtggagac atctttgttc atttgaaaat
      121 attattatct aagtaaaaaa taagaataca cagtatcagg aggaatagta tggagttaag
      181 tgtagaacgt ttaacaaaac agtataacaa taaaattata gtagataaaa ttgactttaa
      241 attaaatgag ggtatatatg gatttttagg agaaaatgga gcgggaaaaa caactcttat
      301 gagaatgatt tgcggaatat taagtcctac atctggagaa gtaaaggtgg atggtaaaaa
      361 taatttggat atgggagaag attttagaga tatgattgga taccttcctc aaaactttgg
      421 atactaccca gattttactg ccaaagaatt tatgctatat atagcatcat taaaaggatt
      481 aatgcctagc tatgcaaaga gaaaaacaca agaattatta gaaatggtag gattatcaga
      541 ggtaattaat aaaaaaataa gaacattttc tggaggtatg aagcaaaggc taggatttgc
      601 tcaaacacta cttaatgacc caaaaatttt aatattagat gagccaacag cagggctaga
      661 ccctaaagaa cgagttaaat ttcgcaacct aatttcagaa ttttctaagg aaaagattgt
      721 tatattgtct actcatattg tgtctgatgt agagtatatt gcagataaaa ttttaataat
      781 gaaaaaagga agatttgttt taactggctc agtagaagaa gtaacttcaa agataaaaga
      841 taaggtatgg aaatgtgtgg tcaatgagaa tgaaatagat tatatgtctg atagatattg
      901 tttagcaaat atccatcatt ctggtggagt tgcaaatctt agaattgtaa gtgatataaa
      961 gccacatgac aatgcatgca atttaaaacc aacattagaa gatttatatt tatattgttt
     1021 caacgaagta ctataaatat acataaggag ataggtatgt ctacattaat taaatttgag
     1081 ttaaaaaaaa taataaaaag aaagtctaca atagcagtta ttttaggaag tttattctta
     1141 agcatcattc tttttgcatt aatgccaatt caggaaaaag ctaatgatga aagtggaaaa
     1201 gagtataggg gtttacaggc tattgagcta aaaaagcaac aatctaaatt aaatgaggga
     1261 tacataacag ataaaaaagt aaaaaactca atcagagatt accaaaaaat gtttaataat
     1321 ccaaataaca ttgtaacaaa tgagtctgga ttctcagatt ttaagccaga tatttattgg
     1381 aaattcttct atccaaataa aactttttat aattttataa tagagtcata tagtaaacca
     1441 aatgatttta acccagaagt aattaaatca atgccattag aaaatgaaat taatttttat
     1501 aaatttagaa atgaaaaagt aaatacaata ttaaatatgg aatatccaga gggaaattac
     1561 acacagaaag aaaaggactt ttggttatct aaaaataagg atataaagga gccttattta
     1621 aatggatatt atcaagggtg ggaatatttt ctattggcaa gagatttact gatttttact
     1681 ataatggctg tctgtataag tatagcttct gtattttcag gagaatacca atctggtgct
     1741 gattcagtag tattgacttc taaatatgga aaaacaaaag ttataacagc taaaataata
     1801 agttccttct tatttggtac gattattttt acaataaata caattgtatc tattggcata
     1861 cttcttttat cttttggaac tgatggctgg aatttaccac tacaacttac aactagttca
     1921 attccatacc catatacatt tttacaggca atattactaa gtgtgttaac tatctatatg
     1981 gtattaatag tattaattag ttttacactt ttattatcgt cttatatgaa atctcctttt
     2041 ccagttttaa ttacaaatat aatattgtta tttgtaccaa tatttttcaa atacagtgaa
     2101 acaaataagt tgtataatca aattctcaat gttttaccat caaatgctat ttcattttca
     2161 tttactaatt atgtagatta taaatttggt aatttaataa tagctttacc tacaatgatt
     2221 atttttacat gcttagttct tac
//
LOCUS       DAELLV010000356         2240 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.358, whole genome shotgun sequence.
ACCESSION   DAELLV010000356 DAELLV010000000
VERSION     DAELLV010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.358"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            801..1463
                     /locus_tag="KPD58_003578"
     CDS             801..1463
                     /locus_tag="KPD58_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0267301.1"
                     /translation="MSILEIKNLNKIYGKSETEVKALSQINLKINSGEFVVIVGKSGS
                     GKSTLLHIMAGLETPTNGDVIIDNINISSLDEKKRSALRKEKLGLIFQSYNLIPVLTV
                     EENIKLPTINIKNKDEAYINELMELLGIKDRRMYLPNQLSGGQQQRVAIARALVNKPS
                     IVFADEPTGNLDTKTESDVLSLLKESQKKYNQTIIMITHNIDITKYADRVIEIEDGFI
                     RE"
     gene            1472..>2240
                     /locus_tag="KPD58_003579"
     CDS             1472..>2240
                     /locus_tag="KPD58_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0267302.1"
                     /translation="MKLNYIVKKYIMGNKKNTLLIVVSIVISTALFLIMNIISEDATN
                     LMINQAKNEFTLKHAQYINPKDEEIKYIENNTSIDKVGKSMLLGISDIGKGQTLQILS
                     QDKVAEELNDIYTLEKGKLPVAENEIAIDSWYIKQKKIKNPIGKRITLDYRRQGIDQE
                     DLYTGEKEFKITGILNSNPILKAQGTSIGLISNDCAIKNIPTKNKYDQVMFTFKKEKN
                     IQRQSQKLIKNGNLNENNIYFNNELLIAISDSMNLKIP"
BASE COUNT         1010 a          199 c          356 g          675 t
ORIGIN      
        1 atgtttaaat cagaaatata aataaatatt attataatcg ttattaaaga ccaaaaatcc
       61 caccttagaa gtttgtatca tatgaaacct ttatagatta tgaaagcaat aatatagtgt
      121 atgatatata aaataagtta taaaagtatt tttttagata ttcaaaaaga cattgtaagt
      181 tatattaata atatgggtat caacgaattt tggatataat gagtttttca acttaaagtt
      241 atgtaataat gaatgtaaag aatatttaat gaaatcattt tgttggttat gtctagctga
      301 atacaataat cttttagata aagattttat atttgaagat aggtgtgaaa aaattaagaa
      361 gatattaaca tcagcagttt agaggcaaag catgtaaagt aaagtctggg taactaaaag
      421 tagtaattta gattgtaact attgttataa atatgaaaat gaggaaacag gaaatataag
      481 ttaggatgta gaaaataggt tactaaataa aaagcttaat tatataatca aagaaatagg
      541 tatatactgt acaatttcat ggtgaagaac ttttcctaaa taaaaaggtt ggtaaaagag
      601 atataagaaa taaaattcaa ttataagata tggtatgata actaaaggca ctatatggag
      661 tgaataggcg gaagaatttt ttgaaaatta taaaaatagt ttctgttgct acatatcaat
      721 aagtatagat agagattgca aaatccatag caaaaatcga atttataagt gtaaataaca
      781 tgtaagacaa ggagtaaatt atgagtattt tagagataaa aaacctaaat aaaatatatg
      841 gtaaatcgga aacagaggtg aaagcgttaa gtcaaataaa tctaaaaata aatagtggag
      901 aatttgttgt gatagtaggt aaaagtggaa gtggtaaaag tactttacta cacataatgg
      961 caggacttga aactccaaca aatggtgatg tgattattga taatataaat atatcatctc
     1021 ttgatgaaaa gaaaagatct gcattacgta aagaaaagtt gggattaata tttcaatcat
     1081 ataatttgat tccagtttta actgttgaag aaaatataaa actaccaact ataaatataa
     1141 aaaataaaga tgaagcttat attaatgaat tgatggagtt actaggaata aaagatagaa
     1201 gaatgtattt gccaaatcaa ttatcaggtg ggcaacaaca aagagtagca atagcaaggg
     1261 ctcttgtaaa taaaccatca attgtatttg ctgatgaacc tactggaaat ttagacacaa
     1321 agacagagtc agatgtatta agtttattaa aagaatctca aaaaaaatat aatcagacaa
     1381 taataatgat aacacataac atagatataa caaaatatgc tgatagagtt atagaaatag
     1441 aagatggatt tataagagag taggtagatt tatgaaactt aattatattg taaaaaaata
     1501 tataatggga aataagaaaa atacattatt aatagttgta agtattgtaa tttcaacagc
     1561 gttgttttta attatgaata taataagtga agatgcgaca aacttaatga taaatcaagc
     1621 taaaaatgaa tttacattaa aacatgcaca atatatcaat ccaaaagacg aagaaataaa
     1681 atatatagaa aataacacaa gtatagataa agttggaaaa tcaatgcttt taggaattag
     1741 tgatattggt aaaggtcaaa cactacaaat tctatctcag gataaagttg ctgaagaact
     1801 aaatgatatt tacacactag aaaaaggtaa attaccagta gctgaaaatg aaatcgctat
     1861 agattcatgg tatattaaac aaaaaaagat aaaaaaccct ataggaaaaa ggataacatt
     1921 agattataga agacaaggga tagatcaaga agatttatat actggggaaa aagaatttaa
     1981 aataactggt atcttaaata gtaatcctat tttaaaagct caaggaacat caattggcct
     2041 tataagtaac gattgtgcta tcaagaatat tcctactaaa aataaatatg atcaagttat
     2101 gtttactttt aaaaaggaaa aaaatataca gagacaatca caaaaactta ttaaaaatgg
     2161 aaatttgaat gagaataaca tttattttaa taatgaatta ttaatagcta tatctgatag
     2221 tatgaattta aaaatacctt
//
LOCUS       DAELLV010000357         2235 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.359, whole genome shotgun sequence.
ACCESSION   DAELLV010000357 DAELLV010000000
VERSION     DAELLV010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.359"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            6..1889
                     /locus_tag="KPD58_003580"
     CDS             6..1889
                     /locus_tag="KPD58_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG0267303.1"
                     /translation="MVLGSTSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTE
                     SYEGTINYNKDPEYLNVWELQGITINSKNNPKTLNKQELEKMGLNLKDYNVTQECIIE
                     DITSRKDVNKYLRKTSAPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGI
                     ISTPLATTYNAPILLVEKNNVPNSVKSELKRLNPRDVIIIGDENAISKTTANQIKSTV
                     NASQTRLKGSNRYETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPI
                     ILTDKNSITDNTYKWLKSEDLQNAYFIGGPQMISTNVINKVNDITKDNVTNNRVYGAD
                     RHETNANVIKKFYTDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYH
                     KENLEAKSANKVYKIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGS
                     DTGTTGKPLGGIREKDYTLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSL
                     RPDLFTSIHYNASDTTGNGVEVFYKLKDKDGGTTKTVATNILNRILEKFNLKNRGAKT
                     RTLSTDPTKDYLYVLRNNDMPAVLVECAFLDNEKDMSLLNTSNKVKEMGTQIGKGIED
                     SLK"
BASE COUNT          933 a          275 c          387 g          640 t
ORIGIN      
        1 aaagtatcgt tttaggtagt acctcaaaag tcagtgttaa gtttaaagaa aaaccagatg
       61 cagatagcat tacattaaag tataaatgct atgatatgcc attggataca actttaaact
      121 ataatcaatc aactgagtca tatgaaggaa ctatcaatta taataaagac ccagaatatc
      181 taaatgtttg ggaactacaa gggataacaa taaacagcaa aaataatcct aaaactttaa
      241 acaaacaaga actagaaaag atgggattaa atttaaaaga ctataatgta acgcaggaat
      301 gtataattga agatataact tctagaaaag atgtaaataa atatttaaga aaaacttctg
      361 cacctattac agaacttaca ggaagtgata gatacgaaac agcagttaaa ataagtaaag
      421 aaggctggaa aaatggttca gataaggtag ttataataaa tggggatgta agtatagatg
      481 gcattatatc aactccacta gcaactacat ataatgcgcc aatacttttg gttgaaaaaa
      541 acaatgtacc taatagtgta aaatcagaat taaagcgttt aaatcctaga gatgtaatta
      601 taattggaga tgagaatgct atttctaaaa ctactgctaa tcaaattaaa tcgactgtaa
      661 atgctagtca aacacgtcta aagggctcta atagatatga gacatcttta ttgatagcaa
      721 aggaaataga taaaaatcat gatgtggaaa aagtatacat aacaaatgct aatggcggag
      781 aagtggatgc acttactata gcagcaaaag caggtcaaga caagcaacca attatattaa
      841 ctgataaaaa tagtattaca gacaacacat ataaatggtt aaagagtgag gatttacaaa
      901 atgcttattt tataggtggc cctcaaatga tatcaacaaa tgttataaat aaggtaaatg
      961 atataactaa agataatgtt actaataata gagtatatgg agcagataga cacgaaacaa
     1021 atgcaaacgt aataaaaaaa ttctatacag atgatgagtt agaggctgtt ttagtagcta
     1081 aatcagatgt acttgttgat gctttagcag caggtccatt ggctgcgaac ttaaaatctc
     1141 caatacttat aacaccaaag acatatgtat ctgcatacca taaagaaaac ttagaagcta
     1201 aatcggcaaa taaggtatat aaaataggtg gaggattgac ttctaaggta atgagctcta
     1261 tagcatcatc attatctaaa cacaatacaa ctccaacaga accaggaaat agtgggggca
     1321 agacagttat gattgaccca ggacatggtg gttcggacac tggaacaaca ggtaaaccat
     1381 tagggggtat tagagaaaaa gactatacat taaatacttc acttgcgaca actgaatatt
     1441 tacgttcaaa gggattcaat gtaataatga caagagacac agataagact ttatctcttg
     1501 gaaatagaac tgctttatct aactctttga gaccagattt atttacgagt atacactata
     1561 atgcatctga tacaactggg aatggtgtag aagtatttta taagcttaaa gataaagatg
     1621 gagggactac taaaactgta gctacaaata tattaaatag aatcttagag aaatttaatc
     1681 taaaaaatag aggtgcaaaa acaagaacac tatcaactga ccctacaaaa gattatttat
     1741 atgttttaag aaataatgat atgccagctg tacttgtaga atgtgcattt ttggataatg
     1801 agaaagatat gagtttactt aatacatcaa ataaagtaaa agaaatgggt acacaaatag
     1861 gtaaaggcat agaagattca ttaaaatagt ctacaatgag agcatagaaa aatttaaaat
     1921 aaattaatct gcaattttaa atttaaacta aagctataag ataagaatgt aattataata
     1981 gtaataaaga ctgtatatac attgtgcata taagtgcgaa atgaggtata tacagtcttt
     2041 ttatgattaa ttgtaaaagg atttatatta tatgaagaat ttacatatct taaggctaca
     2101 gttaattgtc aagagttgga gtattcattt gtaatacaca ataaaagctt aattactata
     2161 ttttattgtg tgtttgtgtg ttttttgtgg atttttatgt caataaatga tatagtatag
     2221 ataattaaat gtatt
//
LOCUS       DAELLV010000358         2232 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.360, whole genome shotgun sequence.
ACCESSION   DAELLV010000358 DAELLV010000000
VERSION     DAELLV010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.360"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            99..1625
                     /locus_tag="KPD58_003581"
     CDS             99..1625
                     /locus_tag="KPD58_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG0267304.1"
                     /translation="MNESYWFLNSSPKEYNKLGENIKTDCLIVGGGITGLTTAYLLAK
                     EGKKVVLVEADKIGYGTSGRNTGKVTCQHDIFYSKIEKKYGLDKAKSYYNANNEALNL
                     VEQIIEENNIKCDFKRETSFVFTEKEDTIKDIKDEYRTCKKIGINCEYHETIENIPLD
                     IKGAISFTNQGQFNPKKYIDGLAKAAVNLGLKIYENTPVVDLEKGKICRVKTREDNII
                     EAENVIISSHSPWYDGLNFYFAKEYAERAYLMVAVLENKLADGMFISIDDPSITFRQY
                     NDGSENLLIFGGGDHKVGQGGTEKEIFDDLEHYGKEVFKVKDFKGKWSAQDNMSFDNV
                     PYIGYINKREDNIYVATGFSKWGITNGTAAGIIIKDLIINNNSDYKDTFNPSRLGSYF
                     SKDFIKENANVAINYVSGKLKIGSGDMPKNNGEGKIVNIDGKRYGVYKDDNGDFYIVD
                     TTCTHLGCELNFNSEEKTWDCPCHGSRFDYKGNILEGPALKPLKLYGHGDNDVNPKLL
                     "
     gene            1653..2210
                     /locus_tag="KPD58_003582"
     CDS             1653..2210
                     /locus_tag="KPD58_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0267305.1"
                     /translation="MNKLLKPITILLYIVYILSAVFFVANKEYGSLGLVAISLVGTFI
                     LSFINKQTERLLSKELYIMLALFIMFSSLLGTCFNFYDINYYDDFLHLWSGIISCIVA
                     FSIIKYFYTQREISKMSKVFLVIFLFMFSMGVASLWEIGEFLMDTFVGTTTQAGGLED
                     TMIDMVDALIGTIITIPFIVKKSNI"
BASE COUNT          863 a          192 c          416 g          761 t
ORIGIN      
        1 tttagctttt taataagctt attttttata ctatttataa taaataaact ccatgatggg
       61 aaaactatta ctatttaata tgtaaaggag tattttaaat gaatgaatca tattggtttt
      121 taaactcaag tccaaaagaa tataacaaat taggtgaaaa tataaaaaca gattgtctta
      181 tagtaggtgg tggaataacg ggattgacaa ctgcatattt attagcaaaa gaaggtaaaa
      241 aagtagtgct tgttgaggct gataaaatag gatatggaac ttctggaagg aatactggta
      301 aggtaacttg tcagcatgat atattttact ctaagataga gaaaaaatat ggattagata
      361 aagcaaaatc atattataat gcaaataatg aagctttaaa tttagttgaa caaataatag
      421 aagaaaacaa tataaaatgt gattttaaaa gggaaacttc ttttgtattt acagaaaaag
      481 aggatactat aaaggatatt aaagatgaat atcgtacatg taaaaaaata ggaataaatt
      541 gtgagtatca tgaaactata gaaaatattc cattagatat aaagggggct attagtttta
      601 ctaatcaagg tcaatttaac ccaaagaagt atattgatgg attggcaaaa gcagcagtaa
      661 atttagggct aaaaatatat gaaaatacac cagtagtaga ccttgaaaaa ggaaaaatat
      721 gtagggttaa aacaagagag gataatataa tagaagctga gaatgttata atttcatctc
      781 atagtccatg gtatgatgga ttgaattttt attttgcaaa agaatatgca gagcgtgctt
      841 atttaatggt tgcagttttg gaaaataaat tagcagatgg tatgtttata agtatagatg
      901 acccatcaat aacttttaga caatataatg atggaagtga aaatttactg atttttggag
      961 gaggagacca taaagttggt cagggaggaa ctgagaaaga aatatttgat gatttagagc
     1021 attatggtaa agaagtcttt aaggtaaaag attttaaggg gaaatggtct gctcaagata
     1081 atatgagttt tgacaatgtt ccttatattg gttatatcaa caaaagagaa gacaatattt
     1141 atgtagctac agggttctct aaatggggaa ttactaatgg aactgctgct ggaataataa
     1201 taaaagattt aataataaat aataattctg attataaaga tacttttaat ccatccagac
     1261 taggtagcta cttttcaaaa gattttataa aggaaaatgc aaacgtagca ataaattatg
     1321 taagtggaaa attaaaaata ggtagtggag atatgcctaa aaataatgga gaaggaaaga
     1381 ttgttaatat tgatggaaaa agatatggtg tatataagga tgataatgga gatttttata
     1441 ttgtagacac tacatgtact catttaggat gtgaacttaa ttttaacagt gaagaaaaaa
     1501 catgggactg tccatgtcat ggttctagat ttgactataa gggaaatata cttgaaggac
     1561 cagctcttaa gccattaaaa ctttatggac atggtgataa tgatgttaat cctaagttat
     1621 tataaaatta tttttaggaa ggtaaagtgt atatgaataa acttttaaag ccaataacta
     1681 tactattgta tatagtttat attttatctg ccgtattttt tgtagcaaac aaagaatatg
     1741 gaagtctagg tctagtagca attagtttag ttggtacttt cattttgagt tttataaata
     1801 agcaaactga aagattattg agtaaagaat tatatataat gcttgcttta tttataatgt
     1861 tttcatcatt gttaggtact tgttttaatt tttatgatat aaattattat gatgattttt
     1921 tgcatttatg gtcaggtata atatcttgta ttgtggcttt ttctataatt aaatactttt
     1981 atacacaaag agagataagt aaaatgagta aagtgttttt agttatattt ttatttatgt
     2041 ttagtatggg agtagcgagt ttatgggaaa taggtgaatt tttaatggat acttttgttg
     2101 ggactactac acaggccgga gggttagaag atactatgat tgatatggta gatgctttaa
     2161 taggcacaat tataactatt ccttttatag taaaaaaatc taatatatag ttttaaattt
     2221 tttatattaa tt
//
LOCUS       DAELLV010000359         2227 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.361, whole genome shotgun sequence.
ACCESSION   DAELLV010000359 DAELLV010000000
VERSION     DAELLV010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.361"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(143..379)
                     /locus_tag="KPD58_003583"
     CDS             complement(143..379)
                     /locus_tag="KPD58_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0267306.1"
                     /translation="MLLTHIYFNGQFKQTIELYKQAFNATVKTLIEDSCYLLIVHNEI
                     VIHNKFLILNDFGNKNGVSISEGYQPTDKIQTAS"
     gene            complement(534..758)
                     /locus_tag="KPD58_003584"
     CDS             complement(534..758)
                     /locus_tag="KPD58_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG0267307.1"
                     /translation="MTVYNLKLGQKGIIDNIAGNEKLMKRLLALGLIDGTEVEVKKIA
                     PLNDPIVIRFRGFDLAIRKSDAKNINLKNN"
     gene            complement(855..2108)
                     /locus_tag="KPD58_003585"
     CDS             complement(855..2108)
                     /locus_tag="KPD58_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HBG0267308.1"
                     /translation="MITLSFDIVQTLTLSIFFFLIGNLLKNKVNFLNNFCIPAPVIGG
                     LLFCFLNLFFKYFNIADISISGNLMPNFITFFFTTIGLEISINLIKKGGSVLFRYWIL
                     CGVLAFCQNILAITISKIIKLEPLLGLMCGNVSMEGGHGYSAAFGLTIENLGIEGAVG
                     VGLSAATIGLIMGGILGCPVAKFLINKYKLKPSSNIDLSIPRYNRNLKRFGSKFFRNK
                     NRIKNNNFSETITPSVFLEQVLLIFICINTGEIISRCFYITFNILLPSVVTCMFSAVI
                     FRNLNDKINILELNFKLIDFLKELSLGIFLTLSLMNIDLFELSTLLPPILLIVTFQVI
                     FIILFSIFICFRVLGKDFDSAIIISGLIGHGIGATPNALANMSSLTQKYGDSPKAFLV
                     VPLVSGFLLDAISIPCILFFINILT"
BASE COUNT          852 a          321 c          248 g          806 t
ORIGIN      
        1 ttatttacaa gtctatattg ataactttta catagtatat aatgtttaaa tacttattca
       61 aatttatcta tacataaaca aaaaaagaga ttttaaaatc tctttttaga ttgttgactt
      121 atggcttttt gatttaataa aatcaagaag ccgtttgaat tttatctgtt ggctgatacc
      181 cttctgatat tgaaacacca tttttgtttc caaaatcgtt taaaattaag aacttattat
      241 gaatgactat ttcattatga acaataagca gataacatga atcttcaatc aatgttttaa
      301 cagtagcatt aaatgcttgc ttatacagtt ctatcgtttg tttgaactgt ccattaaagt
      361 aaatatgagt caataacata actatatcta cacaaattaa tataaatttt ttatactgta
      421 cattatctaa ctgttgatta tctgtattac aacatgattg aatttaaaaa gccatcaaca
      481 catagaatgt tgatggcctg aaaatagatt taaaatctct tttttattat atattaatta
      541 ttttttaaat ttatattttt tgcatcagat tttctaatag ctaagtcaaa ccctctaaat
      601 ctaatcacta tagggtcatt aagaggagct atctttttca cttcaacttc agtgccatct
      661 attagtccta aggctagaag tctcttcata agtttttcat ttccagctat attgtctata
      721 attccctttt gtccaagttt taagttgtaa acagtcatta ttaacacctc ctaatgaaaa
      781 tcattatcaa actttatatt tataatatat aacaaaaaga gcaaattgtc aatatataac
      841 aaaaaataaa catactaagt tagtatgttt ataaaaaata gtatacatgg gatactaata
      901 gcatctagca aaaaaccact tactaatggt acaactaaaa aggcttttgg agaatcacca
      961 tatttttgtg tcaaagaact catatttgcc agagcgtttg gagtagctcc tattccatgt
     1021 ccaatcaaac cacttattat gatggcagaa tcaaaatctt ttccaagtac tctaaagcaa
     1081 ataaatattg aaaataggat tataaagatt acttgaaaag ttactattaa gagaatagga
     1141 ggtaataatg tagataattc aaataaatct atattcataa gtgataaagt taaaaaaatt
     1201 cccaatgaaa gttcttttaa aaaatctata agtttaaaat ttaattctag tatatttatc
     1261 ttatcattta aatttctaaa aataacagcc gaaaacatac aagttacaac agagggtaat
     1321 agtatattaa acgttatata gaagcatcta cttataattt ctcctgtatt tatacaaata
     1381 aatatgagaa gaacctgttc caaaaataca gatggtgtta ttgtttcgga gaagttatta
     1441 ttttttatcc tattcttatt tctaaaaaat ttacttccaa atctctttag atttctatta
     1501 tatctaggaa tcgacaagtc tatatttgat gatggtttta atttatattt atttattaga
     1561 aatttagcta caggacatcc caatatacca cccataataa gtcctattgt tgcagcagat
     1621 aatccaaccc caactgctcc ttctataccg agattttcta ttgtaagtcc aaatgcagca
     1681 gaataaccat gaccaccttc catagataca tttccacaca taagccctaa aagaggttca
     1741 agttttataa tctttgatat tgtaatagct aatatatttt gacaaaatgc caatactcca
     1801 cacaaaatcc aatatctaaa taatacacta ccacctttct ttataagatt tatgcttatt
     1861 tcaagtccta tagttgtaaa aaagaaggtt atgaaatttg gcattaaatt tccacttata
     1921 gatatatctg ctatattaaa atatttaaaa aataaattta gaaaacaaaa taatagacct
     1981 cctattactg gtgcaggtat acagaaatta tttaaaaagt taactttatt cttcaataaa
     2041 ttaccaatta aaaagaagaa tatagatagt gtcaaggttt gcactatatc aaaacttaag
     2101 gtaatcaaaa taagtcctcc tttgtaattc aaagttttaa ttatataact taaattctta
     2161 tgtattgaaa ttttattatc aaatgtatat taataaatgt atattaaaaa gaatcagtag
     2221 atataat
//
LOCUS       DAELLV010000360         2185 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.362, whole genome shotgun sequence.
ACCESSION   DAELLV010000360 DAELLV010000000
VERSION     DAELLV010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.362"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            32..685
                     /locus_tag="KPD58_003586"
     CDS             32..685
                     /locus_tag="KPD58_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBG0267309.1"
                     /translation="MTKIIKVQKPKFTGELEIIENLEDILEDIFNDEKIFNKIIENEV
                     ISDLDSVRVSFESCIFRNVSFENCNLKKIDLLDVVFENCNLSNTFFDGGSIYRVEFKN
                     SKLIGVRFDDCILKNVLFKDVLGKYSNFSFSKFKIVSILDSNFENSVFQEVKGDNLVF
                     EYTNLKKANFNGTKLSGIDFTTNNIEGIEIGIDDIRGAIFDVSQAIDLTKLMGIIIK"
     gene            826..2097
                     /gene="brnQ"
                     /locus_tag="KPD58_003587"
     CDS             826..2097
                     /gene="brnQ"
                     /locus_tag="KPD58_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBG0267310.1"
                     /translation="MRDIFIVGFALFSMFFGAGNLMFPPFLGMESGKDWLIPLIGFVV
                     ADAGIAMLVIIATARCKGSMDDVLRRAGKGLARVMSVAALACLALLVIPRTCATTYEM
                     GIMPIFGDHGPVAKAVFSIIFFGLTLAFTIRSSKVIDIIGKYLTPALLLVLFVLIVKG
                     IISPAGPMSPEHMIDKNLFGEGISQGYQTMDALGAASMATIMLMSIIAKGYTSDKDQI
                     GMTIKAGFVACVFLAVVYGGLTYLGATVSTLYDTSIPQASLLVEITSILLYGQVGKVI
                     LGVIVALACLTTSTGLTASISTYFEGISKASYKQLVIGICVASMLISNLGVDSIIAIS
                     VPILQTIYPVLLAIVVMELAGKHIKNDNAFKGAAYVTLVISLLSAINGMTGAVPFIQS
                     LPLAGLGFNWVIPAILGGIVGNFIKSNKQVA"
BASE COUNT          758 a          239 c          409 g          779 t
ORIGIN      
        1 aaaaataata taatttatag tggaggggaa aatgactaaa attattaaag tgcaaaaacc
       61 taaatttaca ggagaattag aaattattga aaatcttgaa gatatacttg aagatatatt
      121 taatgatgaa aaaatattta ataaaattat agaaaatgaa gttataagtg atttagattc
      181 tgtaagagtt agctttgaat cttgtatatt tagaaatgtg tcttttgaaa attgtaattt
      241 aaaaaagatt gatttacttg atgtggtatt tgaaaactgc aatttatcaa atacattttt
      301 tgatggagga agtatataca gagttgaatt taaaaatagt aaattgatag gagttagatt
      361 tgatgattgt atacttaaaa acgttttgtt caaggatgtt ttaggtaagt attctaactt
      421 ttcattctct aaatttaaga tagtttctat cttagattca aattttgaaa attctgtatt
      481 tcaagaagta aaaggtgaca atctagtatt tgagtataca aatttaaaaa aagcaaactt
      541 taatggaact aaactttctg gcatagattt tactactaat aatattgagg gaattgagat
      601 tggtatagat gatattagag gagcaatttt tgatgtgtca caagcaattg atttaacgaa
      661 attgatggga attatcataa aatgaaaaaa atattcataa aataaattgg gaaaacgtta
      721 acaatgtgtg aatttttacc agattgacat ttttctgaaa ataaagtaag attatcttaa
      781 tattcaaaat attttcaaag ggggtaattt agtgtgagta agcagatgag agatattttt
      841 atagttggtt ttgctttatt ttctatgttc tttggtgcag gaaatcttat gtttcctcca
      901 tttttaggga tggaatccgg caaagactgg ttaattcctc ttattggatt tgttgttgca
      961 gatgctggta tagcaatgct tgttattata gcaacagcaa gatgtaaggg aagcatggat
     1021 gatgtactta ggagagctgg taaagggtta gcaagagtaa tgagtgtagc tgctctagca
     1081 tgtttagcac ttcttgtaat accaagaact tgcgcaacaa catatgaaat gggtattatg
     1141 cctatatttg gagaccatgg tccagtagct aaagctgtat tttcaataat cttctttggg
     1201 ctaactttag catttactat taggtcttct aaggttatag acataatagg taagtattta
     1261 actccagcgt tattattagt attatttgtt ttaatagtaa agggtataat ttctccagca
     1321 ggacctatga gtcctgagca tatgatagat aaaaatctat ttggtgaagg tatatcacaa
     1381 ggttatcaaa caatggacgc tctaggtgca gcatctatgg caactataat gttgatgtct
     1441 ataatagcta aaggatatac atcagataaa gaccaaatag gaatgactat taaagctggt
     1501 tttgtagcct gtgtatttct agcagtagta tatggtggtc ttacttattt aggtgcaaca
     1561 gtttcaactt tatatgatac tagtatacct caagcatctt tattagttga aataactagt
     1621 atattgttat atggtcaagt aggaaaggta attttaggtg taattgtggc acttgcctgt
     1681 ctgactactt ctacaggttt aacagcatca atatcaactt attttgaagg aataagtaag
     1741 gcgagttata aacaactggt tataggtata tgtgtagcta gtatgcttat atcaaattta
     1801 ggtgttgatt ctataatagc catatcagtt ccaatcctac aaactatata tcctgtttta
     1861 ttagcaatag ttgttatgga gttggctggt aaacatataa agaatgataa tgcttttaaa
     1921 ggtgcagcat atgttacatt agtgattagt ttattatcag ctataaatgg aatgacagga
     1981 gctgttccat ttatacaaag cttgccactt gcaggtttgg gctttaactg ggtaatacca
     2041 gctatattgg gtggaatagt tggtaacttt ataaaaagca acaaacaagt agcataataa
     2101 tttgtaatta atttaatttt ataatatatt aatttttagt actatgggta ggtttaatct
     2161 aaacctaatc tacccatagt acttt
//
LOCUS       DAELLV010000361         2059 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.363, whole genome shotgun sequence.
ACCESSION   DAELLV010000361 DAELLV010000000
VERSION     DAELLV010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.363"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            188..733
                     /locus_tag="KPD58_003588"
     CDS             188..733
                     /locus_tag="KPD58_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HBG0267311.1"
                     /translation="MKNTKVNIQDLTKMSICVALLCISSYIYIPLPFTPAGVTAQTIM
                     INLIALILTPRQAFSTVGVYIMIGLIGIPVFGGGTSGIGKLLSPTGGFYFGFLFAALI
                     ISLLKGRNNDIKRYILITIFVGMPIIYFMGTVFMCYFNQMTVEAALFAAVVPFLIGDT
                     IKSVIASFLGTKLNNVLRRNL"
     gene            765..1901
                     /locus_tag="KPD58_003589"
     CDS             765..1901
                     /locus_tag="KPD58_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HBG0267312.1"
                     /translation="MKEVFILGGLRSHIGLKNGIFQFVQPELLGASVLKDLIKKYEID
                     KIDEIICGNAVGTGGNIARLMTLTAGVSNEVPAFTVDMQCASAMMSIDIAFSKVKSGQ
                     CDLIIAGGFESSSLQPMRTYHKNDKRYDTNNPNYTVAQFSPDDNSQNSMLEGAERVAE
                     LYQIEKADLDFWVKESHKRAKEAREEKILEDIISPINNSTYDEGIRDKMSQRLLDRMP
                     SILGKETITNAANSCLINDGASFIIICSKKYLEHVKKKPKAKIINTCTIGTDATLSPT
                     SAIQAMDKLLEIESLNYLDVSAVEFNEAFAVIDVLFQRKYPELIDRYNIFGGALAYGH
                     PYGASGAIIALHLLKALEKTKGRYGICSIAAAGGLGSALLLERV"
     gene            1905..>2059
                     /locus_tag="KPD58_003590"
     CDS             1905..>2059
                     /locus_tag="KPD58_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HBG0267313.1"
                     /translation="MNYYELLRIKKERQKNKNFLIIDGEKYTYENILSYSEELGKQIS
                     LGENIPI"
BASE COUNT          788 a          224 c          385 g          662 t
ORIGIN      
        1 ttataaatga ctattaaaat ataaagtaac agtataaagt ttacttataa attaaatgta
       61 aacctaaata attataatag ttgacaaaaa aacgtcttta ttttatacta aaacaaagct
      121 ttaatagaag ctttgtatat gtacatataa actaaatgaa ataagacata tgaagggaat
      181 tataaaaatg aaaaatacta aagtaaatat tcaagattta actaaaatgt caatatgtgt
      241 agcattattg tgtatatcat cttacatata tataccattg ccatttactc cagcaggagt
      301 aacagcacaa acaataatga taaatttaat agctttgatt ttaacaccaa gacaagcatt
      361 tagtacagtt ggtgtatata taatgatagg tttaattggg attccagtat ttggtggagg
      421 tacatctggt attggaaagt tgttaagccc aacaggtggt ttttattttg ggtttttgtt
      481 tgcagcacta attattagtt tattaaaagg acgaaacaat gatattaaga gatatatact
      541 aataacaatt tttgttggaa tgcctattat atattttatg ggaacagtat ttatgtgcta
      601 ttttaatcaa atgacagtag aagcagcact ttttgcagca gtagtaccat ttttaattgg
      661 agatacaata aagtcagtta ttgcatcttt tttaggcact aaactgaaca atgtactcag
      721 aagaaatcta tgataaatct ataatataag gaagggtgta atgtatgaag gaagttttta
      781 tattaggagg attgagaagt catataggtc taaaaaatgg aatttttcaa tttgttcaac
      841 ctgaattact aggagcaagt gttttaaaag atttaattaa aaagtatgaa attgacaaaa
      901 ttgatgagat tatatgtgga aatgcagttg ggacaggagg gaatatagct agattaatga
      961 ctttaacagc aggagtgtca aatgaagtac ctgcttttac tgtggatatg cagtgtgctt
     1021 ctgctatgat gagtatagac atagcttttt caaaggttaa atctggtcag tgtgatttaa
     1081 ttattgcagg tggttttgaa agtagttctc tacaaccaat gcgcacatat cataagaatg
     1141 ataagcgata tgatacaaat aatccaaatt atacagttgc tcaattttct cctgatgata
     1201 atagccaaaa tagtatgctc gaaggggcgg aaagagtagc agagctatat caaattgaga
     1261 aagcagactt ggatttttgg gtaaaggaaa gtcataaaag agcaaaagaa gcaagagaag
     1321 aaaaaatact tgaggatatt atatctccta taaataatag tacctatgat gaaggtatac
     1381 gtgataagat gagtcaaagg ttattagata ggatgccatc aattttagga aaagaaacta
     1441 ttacaaatgc agcaaattca tgtcttatta atgatggagc ttcatttatt ataatatgtt
     1501 ctaagaagta tttagaacat gtaaaaaaga agcctaaagc aaaaattatt aatacatgca
     1561 caataggaac agatgcaacc ttaagtccaa catcagcgat acaagcaatg gataaacttt
     1621 tagagataga aagtcttaat tatttagatg tatctgctgt tgaatttaat gaagcatttg
     1681 cagttataga tgtgttgttt caaagaaagt atccagagtt aatagataga tataatatat
     1741 ttggtggagc tttagcgtat ggacatccat atggtgcttc aggtgcaatt attgcattac
     1801 atcttttaaa agcattggaa aagactaagg gtagatatgg aatttgctct atagctgcag
     1861 ctggtggtct aggttcagct ttactgcttg aaagggtgta gattatgaac tattatgaac
     1921 ttttaagaat aaaaaaagag aggcaaaaaa ataaaaattt tttaattata gatggagaaa
     1981 aatatactta tgaaaatata ttgagttata gtgaggaact aggaaagcaa atttctttag
     2041 gtgaaaatat cccaatttt
//
LOCUS       DAELLV010000362         2025 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.364, whole genome shotgun sequence.
ACCESSION   DAELLV010000362 DAELLV010000000
VERSION     DAELLV010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.364"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..177
                     /locus_tag="KPD58_003591"
     CDS             <1..177
                     /locus_tag="KPD58_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0267314.1"
                     /translation="LKHIFKKYYRGTNTGEMHKGSGLGMAISKEIIEIHKGKIYVSSE
                     IGIGTKIIIEIKQN"
     gene            188..1012
                     /locus_tag="KPD58_003592"
     CDS             188..1012
                     /locus_tag="KPD58_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HBG0267315.1"
                     /translation="MDFFKKGISGFTIKILALIFMTFDHIAAFMPQTMQIPIWFHWVG
                     RISAPLFIFMAVEGFYHTSNRKKYISRLYIWSVIMAIGNQIINNVFSHPEGAIIINNI
                     FSTLFLIAIYLQAIEFIKKFRKEKEIKYFIIGLLMIIIPIILGIFTVALLFKVTNRVI
                     ALFMILVPVPFLVEGGPIWIILGIIFYLCRGKKFSLSICYVLMCIFIFTTMSNGDYSL
                     KNSILQNYQWMMIASLPLMLLYNGEKGKSMKYLFYLYYPIHVYILYILGIYLINGF"
     gene            complement(1337..1957)
                     /locus_tag="KPD58_003593"
     CDS             complement(1337..1957)
                     /locus_tag="KPD58_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG0267316.1"
                     /translation="MKVLLINGSPNQYGCTYTALNEITKVLSKHDIKTEILYLGKETI
                     PGCISCASCFETGKCIRNDKVNELIEDLDNIDGIIIGSPVYFSSATGQLTSFLDRLFF
                     IAGSRMATKLGASVVSCRRGGASATFDQLNKYFSISNMPIVSSQYWNQVHGFTPEDVM
                     KDEEGLQTMRTLGENMAWLLKCISAGKKAGIKEPQYEETIMTNFIK"
BASE COUNT          713 a          235 c          299 g          778 t
ORIGIN      
        1 ttgaaacata tttttaaaaa atattataga ggtactaata caggtgagat gcataaaggt
       61 tcaggtcttg gtatggctat ttctaaagaa ataatagaga ttcataaagg taaaatttat
      121 gtaagtagtg aaattggaat aggaacaaaa attattatag agataaaaca aaattaggag
      181 atatgatatg gattttttta aaaaaggtat aagtgggttt actattaaga ttttagcatt
      241 aatatttatg acatttgacc atatagcagc atttatgcca caaacaatgc aaataccaat
      301 ttggtttcat tgggtaggca gaatatcagc accactgttt atattcatgg ctgttgaagg
      361 attttatcac actagtaaca gaaaaaaata tataagtaga ttgtatattt ggtctgttat
      421 tatggctata ggaaatcaaa taattaataa tgtattttca catccagaag gagctataat
      481 tataaataac attttttcaa cattattttt aatagcgatt tatttacaag caatagagtt
      541 tataaagaaa tttagaaaag agaaagaaat taaatatttt attataggat tattgatgat
      601 aatcattcct ataattttag gaatatttac agtagcttta ctttttaaag tcactaacag
      661 agttattgct ttatttatga ttttggtacc agtaccattt ttagtagaag gtggaccaat
      721 ttggattata cttgggatta ttttttattt gtgtagaggg aaaaagttca gtttgagtat
      781 ttgttatgtt ttaatgtgta tatttatatt tacaacaatg tcaaatggag attatagttt
      841 aaaaaattct attttacaaa attaccaatg gatgatgatt gcttctctac ctttaatgtt
      901 actttacaat ggagaaaaag ggaagagtat gaagtattta ttttatttat actatccaat
      961 ccatgtatat atattgtata tcttaggaat atacttaata aatggttttt gattgtaagt
     1021 gatattttta atacaaaatg aacaatatga ggattgtttt aggtctatta aaattgttat
     1081 ttagatgaga tttgtagaat atttaaattt atgagcagaa attcagttat tgtaatttca
     1141 ataacattag aatataatat tataatttat ttagaaatat atgatgttat acatggtttg
     1201 gttattaatg taataataaa acagaggttt gatataaaat tcaatacctc tgttttagtg
     1261 ctttttatcc tacatttatg cttattttat ttataaatta gtgttttgtt ttaattaata
     1321 ttagtgcatt ttttatttat tttataaaat tagtcataat tgtttcttca tattgtggtt
     1381 ctttaattcc agctttttta cctgctgata tacacttaag aagccatgcc atattttctc
     1441 ctagagttcg catagtctgt aatccttctt catccttcat aacatcctct ggagtaaagc
     1501 catgtacctg attccaatac tgtgatgata caataggcat attgctgatt gaaaaatatt
     1561 tatttagttg gtcaaatgtt gcacttgcac caccacgtct acatgacaca actgaagcac
     1621 ctaattttgt agccatgcga cttccagcta tgaaaaatag acggtcaaga aaagaagtta
     1681 attgaccagt tgcactagaa aagtaaacag gagaaccaat aataatacca tcaatattat
     1741 caagatcttc aatcaattca ttaactttat catttcgaat acattttcca gtttcaaaac
     1801 aggatgcgca actaatacat cctgggattg tttctttacc taaatacagt atttctgttt
     1861 taatatcgtg tttactcaat actttagtaa tctcatttag tgctgtataa gtacatccat
     1921 attggtttgg gctaccgttt atcaataaaa ctttcattat aaatttcctc ccatgaattt
     1981 tatttaatag attttctaag agatattatt ttttataaaa tagga
//
LOCUS       DAELLV010000363         1967 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.365, whole genome shotgun sequence.
ACCESSION   DAELLV010000363 DAELLV010000000
VERSION     DAELLV010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.365"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            61..342
                     /locus_tag="KPD58_003594"
     CDS             61..342
                     /locus_tag="KPD58_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0267317.1"
                     /translation="MASKQIPRNNLEKFLRIRVLSHTGDKIHIKLPVNFAKRMIENNA
                     LDLFNGKDDVVDGKKVTEILVKAFDYDLTGEVVHLERKNGDIIKVSIVQ"
     gene            606..860
                     /locus_tag="KPD58_003595"
     CDS             606..860
                     /locus_tag="KPD58_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0267318.1"
                     /translation="MENKDFKLTICIFAFVIGVVVLFCCIGLGEFSAEHIIQQNGGIM
                     DTDQYNIYLDQSISQYRNFGSILALLGGIGILFDKYIRKY"
     gene            905..1228
                     /locus_tag="KPD58_003596"
     CDS             905..1228
                     /locus_tag="KPD58_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0267319.1"
                     /translation="MATEIFTVKITPLEAQKMIKENLDADLVFSDSYNLGDDKFILIT
                     TFEKYYARNNSDAGLIVVYENTTGKTVIKATSTGSAIGILKIDWGAGGNLLKRVKNIL
                     ADYII"
     gene            complement(1787..>1967)
                     /locus_tag="KPD58_003597"
     CDS             complement(1787..>1967)
                     /locus_tag="KPD58_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG0267320.1"
                     /translation="VEIIYSEKISEVENMLKIENIQYEFTSRDGFIFFGFYDMTDAYR
                     FETLCSDEKIRFKRI"
BASE COUNT          817 a          160 c          284 g          706 t
ORIGIN      
        1 gatattttta agaataaaat ttattattaa ataaaaaata aaggagagtg atttaatagc
       61 atggcatcaa aacaaatacc aagaaacaat ttagaaaagt ttttaagaat aagagtctta
      121 tcacatacag gcgataaaat acatataaag ttacctgtaa attttgcaaa gagaatgatt
      181 gagaataatg ctttggattt atttaatgga aaagatgatg tagtagatgg taaaaaagtt
      241 actgaaatat tagttaaggc atttgactat gatttaactg gagaagtagt tcacttggag
      301 agaaaaaatg gagacattat taaagtttca attgtacaat agaattattt tatatgaatt
      361 ttatttttga atagttataa ataattagaa atttgtaaaa aagtataata tattattagt
      421 aatggtacag aatacacatt tataatttat agatgtgtat tttttgttta aaaaatttaa
      481 atatataaaa ctataaaggt tacaattttg gaaatagatg taaatatatg ttgtattgta
      541 taataagagt aatataaaat ctagtaaaaa taatcaaata aatttagctt atgagaggga
      601 taactatgga aaataaggat tttaaattaa caatatgtat atttgctttt gtaattggag
      661 tagtagtatt attctgttgt ataggtttag gtgaatttag tgcagagcat attattcaac
      721 aaaatggtgg aatcatggat actgaccaat ataatattta tctagaccaa tctatttctc
      781 aatatagaaa ttttggttct atattagctc tgttaggagg aatagggata ttgtttgata
      841 aatatatcag aaaatactag agattatgtt ttgtctaaaa taaattctta tgaggagata
      901 aaaaatggca acagaaatat ttactgtaaa aattacaccc ttagaggcac aaaaaatgat
      961 aaaagaaaat cttgatgcag atttagtttt tagtgattct tataatttag gagatgataa
     1021 atttatacta attacaactt ttgagaagta ttatgcaaga aataatagtg atgcaggatt
     1081 aatagtagtt tatgaaaata ccacaggaaa aacagttatt aaagctactt caacaggcag
     1141 tgcaattgga attttaaaaa ttgattgggg tgcaggtgga aatttactta aaagagtaaa
     1201 aaacatactt gctgattata ttatttaatt attttatcta actgataata tgtcaagaaa
     1261 atctcgtaaa agaggttaag aacattttta taatatatag ataaataaga atttgaaggt
     1321 aaagttttta aggtataaag aaaaataata taataaaacg aattggcttt ataattcttt
     1381 ataaggcaaa gagcacattt ctttatataa ttgttggata ttaaataata tgtaaataca
     1441 ccatttacat ataaaagttt aattaatatg ttaagagaaa atgaaatgga aattactaaa
     1501 aaatattttt aaattggata atggttgata ttatgtttat acaaaaatca tgtagaattt
     1561 tatatatttt aaatatgata gtttaaaatt atgaaagaat gataaattcc agtttgtagg
     1621 gtaattttga taaataatat taataagtat gagtaaaaag taggagcata ttaatataat
     1681 gtccctactt ttttattatt aaaattgttt ataatatcaa cactacatta aaacacaacc
     1741 taaatttgaa aaacaaatag tttagaaata tgtacttttt tatctattat attcttttaa
     1801 atcgaatttt ttcatctgaa cataaagtct caaatctata tgcgtctgtc atatcataaa
     1861 aaccaaaaaa tataaagcca tcacgagaag taaattcata ttgtatattt tcaattttca
     1921 acatgttttc tacttcgctt attttttcgc tatatataat ttctacc
//
LOCUS       DAELLV010000364         1954 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.366, whole genome shotgun sequence.
ACCESSION   DAELLV010000364 DAELLV010000000
VERSION     DAELLV010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.366"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(14..433)
                     /locus_tag="KPD58_003598"
     CDS             complement(14..433)
                     /locus_tag="KPD58_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0267321.1"
                     /translation="MDTLGERIVYLRKAKNLKQYELEEMLGCDNLSKFERNIRKPNYE
                     ILKSIAEIFNVSVDWLLNGDNLSHKSDLICDSSSNYPLNSINSNEIKLLNNFRKLNDY
                     DKAKIEGMIELKLHEYEKEKDLGKIEYNKNKDEKIDK"
     gene            732..941
                     /locus_tag="KPD58_003599"
     CDS             732..941
                     /locus_tag="KPD58_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0267322.1"
                     /translation="MDARKKWIPFLGVQVKQRLIELNMTQRELAKKIGVNENYLSAIL
                     NGRRTGKKYKSSIYQLLNIEYSEDD"
     gene            973..1182
                     /locus_tag="KPD58_003600"
     CDS             973..1182
                     /locus_tag="KPD58_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0267323.1"
                     /translation="MCKLDSDGVIECCRAIDDFITALSNIKSLNMERLNTLTKYSSTC
                     SILLKEGNYEGCTIVYRKMLEELKT"
     gene            complement(1399..1797)
                     /locus_tag="KPD58_003601"
     CDS             complement(1399..1797)
                     /locus_tag="KPD58_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0267324.1"
                     /translation="MDTLGKRIAYLRNSKKLTQRKLMDILKFENLGKYETGDRKPNCD
                     ILMSIADYFNVTTDWLLYGKEKVNVNSSVKEDKEDYLHVTNDEMTILNLYRQLNERDK
                     IKIEGILELKISEYKDLKKHSSNNNEDKMV"
BASE COUNT          725 a          236 c          246 g          747 t
ORIGIN      
        1 agcaacataa aaattattta tctattttct catctttatt tttgttatac tcaatttttc
       61 ctaaatcctt ctctttttca tattcatgaa gttttaattc aatcatacct tctattttgg
      121 ctttatcata atcatttaac tttctaaaat tgtttaaaag ctttatttca ttagagttta
      181 tgctattaag tggatagttt gaggaggaat cgcaaattaa atctgattta tgtgatagat
      241 tatctccatt taatagccag tctactgaaa cattaaatat ctcagctata gattttaata
      301 tttcataatt tggttttcta atgtttctct caaatttact taagttgtca cagcccaaca
      361 tttcttctag ttcatattgt ttaaggtttt ttgcttttct caaataaaca attctttctc
      421 ctaaagtatc cataaacact ctccattcaa ttaatgtcaa aaagactttt taagatgtaa
      481 atagtttcaa atttaaggtc aaaatgacat aaaaagcatt gacttaaggt caaaatgact
      541 ttataattaa cttaatgata cgaatttaca tcctaatttt agcacaaagt aatcaaaaaa
      601 tcttatttag tattaaataa atttatatac ttaatatgtg tacatattaa aaatatatac
      661 taaatagagg gggtgtgtaa gctaaaataa tataaaagta aatataaatc acttagaaag
      721 gaagttgata aatggatgct cgaaaaaaat ggataccttt tttgggagtg caagtcaagc
      781 aaagacttat tgaattaaat atgactcaaa gggaattagc gaagaaaata ggtgttaatg
      841 aaaactattt gtcagctatt ttaaatggaa gaagaacagg taaaaaatat aaatcatcaa
      901 tttatcaatt acttaatata gaatattcag aagatgatta ataaatagta tataaagtag
      961 gtgaatattc ttgtgtgtaa attggattca gatggggtta tagagtgttg tagagcaatt
     1021 gatgatttta ttacagcact cagtaatata aaaagcttaa atatggaaag attaaatact
     1081 ttaactaaat attctagtac atgttcaatc cttcttaaag aggggaatta tgaaggatgt
     1141 acaattgtgt atagaaagat gttggaagaa ttaaaaacat gagtaatgca tttcttagga
     1201 atataagtta tacatagaga tgtattatat ttttcaaagt acttaaacta aaatatggat
     1261 aagataatct aaatattata aatgtgcttg aaattagact atacttgttt ttaaataatc
     1321 caatatccat attttagtaa tatactacaa aaaaagaagg ttaatagatg atgtaaaatc
     1381 gtatcaaatt atgtatgttt aaaccatttt atcttcatta ttattagagg aatgcttttt
     1441 taagtcttta tattcagata tcttaagttc aagtattcct tctattttta ttttatcacg
     1501 ttcgtttagt tgtctgtata gatttaatat cgtcatttca tcattagtaa catgtaagta
     1561 atcttcttta tcttctttta cactactatt gacatttacc ttctctttac catagagaag
     1621 ccagtcagtc gtaacattaa aataatcagc tattgacatt agtatatcac aattaggttt
     1681 tctatctcct gtttcatact tgcctaagtt ttcaaatttt aaaatatcca taagtttgcg
     1741 ctgagtaagt tttttggagt ttctcaaata agcaattctt tttcctaaag tatccacaaa
     1801 atacactcct ttctttttat gagtaatgtc taaatgacat ttaaaattaa aaatatataa
     1861 atttataata taaaactact aaattaaagt ctaaatgaca ttttgcttaa attaatatgc
     1921 tcataatatg attttaacat attatagttg aaaa
//
LOCUS       DAELLV010000365         1947 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.367, whole genome shotgun sequence.
ACCESSION   DAELLV010000365 DAELLV010000000
VERSION     DAELLV010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.367"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..771
                     /locus_tag="KPD58_003602"
     CDS             <1..771
                     /locus_tag="KPD58_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0267325.1"
                     /translation="YGIGRDSLMYYEEIGILRPVRDINGYRMYNISDIWKLNLIKEFR
                     SLNFPMKKIKEYLDDRSIESTKNILNEELDLIDKKIAEFISHKENIIKRLSSIESVIQ
                     NTKIDEIEVVYIEKRKALELNADIKRDEDFDFLIQKLQKEYEDRFNILGNNNIGSAFS
                     TEAINKGIFNEFKSVFCFLESNEKVYNIVFDEGYYVTLNYTGSNSNNKIYMEKVFKFI
                     EENNYKIIADPIEIYKIDIHETGIVEEFVTEIQVPITK"
     gene            905..1144
                     /locus_tag="KPD58_003603"
     CDS             905..1144
                     /locus_tag="KPD58_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0267326.1"
                     /translation="MKCSNCGSVNIEKGILTTGGIYAETIGLKYRGDGKLDKVINKTF
                     PVQSTIYSDLCLDCGEIVRTYIKDNTDRNWCKDKK"
     gene            complement(1332..1508)
                     /locus_tag="KPD58_003604"
     CDS             complement(1332..1508)
                     /locus_tag="KPD58_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0267327.1"
                     /translation="MFRDEMDKCTHMLTAYISSLYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(1562..1658)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT          808 a          181 c          303 g          655 t
ORIGIN      
        1 tatggaatag gtagagattc attaatgtat tatgaagaaa taggcattct aagaccagtt
       61 agagatataa atggatatag aatgtataat atatcagata tatggaaatt aaatttaata
      121 aaagaattta gaagcttgaa ctttccaatg aagaaaataa aagaatacct agatgacaga
      181 agtatagagt ctacgaaaaa tattttaaat gaagaactcg atttaattga taaaaaaata
      241 gctgaattta taagccataa ggaaaatatt ataaaaagat tgagttcaat tgaaagtgta
      301 attcaaaata caaaaataga tgagatagag gttgtatata tagaaaaaag aaaagcttta
      361 gaattaaatg ctgatataaa gagggatgaa gacttcgatt ttttaattca aaaacttcag
      421 aaagaatatg aagatagatt taatatactt ggaaacaaca atattggttc tgccttttct
      481 actgaagcaa ttaataaagg tatatttaat gagtttaaat ctgtattttg ttttctggaa
      541 agtaatgaga aagtatataa tatagttttt gatgaaggat attatgtaac tttaaattat
      601 actgggagta attctaataa taagatatat atggagaagg tgtttaaatt tatagaagaa
      661 aataattata agataattgc tgaccctata gaaatctata agatagatat acatgagact
      721 ggaattgtag aggagtttgt aacggaaatt caagttccta taactaaata gctatatttc
      781 aatggattag atggtaaata gaatgaaaaa atgttgattt gccactgtgt tataaaatcg
      841 gtagcaaatt atattttaaa ttataagata gcatgaaaaa ataattgttt agggggttat
      901 aaatgtgaaa tgttctaatt gtggaagtgt aaatattgaa aagggaattt taacaactgg
      961 tggcatatat gctgaaacta ttggattaaa atatagagga gatggaaaat tagataaagt
     1021 tataaataaa acttttccag tacaatctac tatatattca gatttatgtt tagattgtgg
     1081 agaaatagtt agaacctata taaaggataa tactgatagg aattggtgta aagataagaa
     1141 atagaataat ttttgaagta tattaatatt tgaagttgaa ttttattaaa gatacaagtg
     1201 aaaaagtata aatgggaaga aattgttata tggaagaaaa ctttgcatat aggggagatt
     1261 agttttttaa tataataaca tttttaaatg tagataaaac taaaaagggt tctctagaaa
     1321 acaatttcta tttaattata cttacttacc aataccatca cttgatgtaa acaagattct
     1381 actacatttt ctccgtactc tagtataaaa tcatctagct gtgtatctat aaaatcacaa
     1441 taatcatata aactactgat ataagcagtt aacatgtgtg tacatttatc catttcatct
     1501 ctaaacatat atattactat ccccctaaaa aactttcata aaacaaaaag aacaatatct
     1561 ttttggcaac tggctgacat aactcatagt ttattttata agcgttagtc cctatagctt
     1621 tgcgtcacta aatttctcta gttttgccga tttagtttta ttctacaact aaaataatac
     1681 actaggataa gttattgttc aacatacttg tcagaaaatt gaaatattta acagtatctt
     1741 aacaatatga aatctatttt tcactcaaga aataaactat acatgtagaa tatttaataa
     1801 aaattatatt tagttttgta ttgaagtttt tgtattaaaa agtttagttc acaagagtta
     1861 cttggctaga taatagatga acaaaaatat cttgaaaaaa tatatatgaa tggcaaaaaa
     1921 caagggatga attttatata gactcat
//
LOCUS       DAELLV010000366         1926 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.368, whole genome shotgun sequence.
ACCESSION   DAELLV010000366 DAELLV010000000
VERSION     DAELLV010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.368"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            120..236
                     /gene="rrf"
                     /locus_tag="KPD58_003605"
     rRNA            120..236
                     /gene="rrf"
                     /locus_tag="KPD58_003605"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            243..317
                     /locus_tag="KPD58_003606"
     tRNA            243..317
                     /locus_tag="KPD58_003606"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:275..277,aa:Asn,seq:gtt)
     gene            322..396
                     /locus_tag="KPD58_003607"
     tRNA            322..396
                     /locus_tag="KPD58_003607"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:355..357,aa:Glu,seq:ttc)
     gene            402..477
                     /locus_tag="KPD58_003608"
     tRNA            402..477
                     /locus_tag="KPD58_003608"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:435..437,aa:Val,seq:tac)
     gene            483..559
                     /locus_tag="KPD58_003609"
     tRNA            483..559
                     /locus_tag="KPD58_003609"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:517..519,aa:Asp,seq:gtc)
     gene            570..644
                     /locus_tag="KPD58_003610"
     tRNA            570..644
                     /locus_tag="KPD58_003610"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:602..604,aa:Thr,seq:tgt)
     gene            659..743
                     /locus_tag="KPD58_003611"
     tRNA            659..743
                     /locus_tag="KPD58_003611"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:693..695,aa:Tyr,seq:gta)
     gene            753..826
                     /locus_tag="KPD58_003612"
     tRNA            753..826
                     /locus_tag="KPD58_003612"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:785..787,aa:Gly,seq:tcc)
     gene            837..913
                     /locus_tag="KPD58_003613"
     tRNA            837..913
                     /locus_tag="KPD58_003613"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:871..873,aa:Arg,seq:tct)
     gene            923..998
                     /locus_tag="KPD58_003614"
     tRNA            923..998
                     /locus_tag="KPD58_003614"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:956..958,aa:Gln,seq:ttg)
     gene            1010..1085
                     /locus_tag="KPD58_003615"
     tRNA            1010..1085
                     /locus_tag="KPD58_003615"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1043..1045,aa:Lys,seq:ttt)
     gene            1088..1176
                     /locus_tag="KPD58_003616"
     tRNA            1088..1176
                     /locus_tag="KPD58_003616"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1122..1124,aa:Ser,seq:tga)
     gene            1194..1284
                     /locus_tag="KPD58_003617"
     tRNA            1194..1284
                     /locus_tag="KPD58_003617"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1228..1230,aa:Ser,seq:gct)
     gene            1293..1369
                     /locus_tag="KPD58_003618"
     tRNA            1293..1369
                     /locus_tag="KPD58_003618"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1327..1329,aa:Pro,seq:tgg)
     gene            1455..1530
                     /locus_tag="KPD58_003619"
     tRNA            1455..1530
                     /locus_tag="KPD58_003619"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1488..1490,aa:Trp,seq:cca)
     gene            1591..1667
                     /locus_tag="KPD58_003620"
     tRNA            1591..1667
                     /locus_tag="KPD58_003620"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1625..1627,aa:Pro,seq:tgg)
     gene            1674..1750
                     /locus_tag="KPD58_003621"
     tRNA            1674..1750
                     /locus_tag="KPD58_003621"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1708..1710,aa:Ile,seq:gat)
     gene            1754..1829
                     /locus_tag="KPD58_003622"
     tRNA            1754..1829
                     /locus_tag="KPD58_003622"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Phe,seq:gaa)
     gene            1837..1913
                     /locus_tag="KPD58_003623"
     tRNA            1837..1913
                     /locus_tag="KPD58_003623"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1871..1873,aa:Met,seq:cat)
BASE COUNT          485 a          443 c          523 g          475 t
ORIGIN      
        1 cattcattaa gatatatgtg cagtttttag agtgttaact ctaaaaaaaa ataaaaaaag
       61 tattgacaaa aatagtaaaa aatgttaata ttatacttgt cctagcaaca agaaaaaaat
      121 gtggttataa tagcggagaa gatacacctg tttccattcc gaacacagaa gttaagttct
      181 ctagcactga tggtacttgg tgggaaactg cctgggagag taggacatag ccacgtgaat
      241 gttccaaggt agctcaacgg tggagcaacc ggctgttaac cggtaggctg tgggttcgag
      301 ccccaccctt ggagccatta tggctcgttg gtcaagaggt taagacaccg ccctttcacg
      361 gcggtaacag gggttcaatt cccctacgag tcaccatttg tgggatcata gctcagctgg
      421 gagagcacct gccttacaag cagggggtca caggttcgag ccctgttggt cccaccattt
      481 gcggcctggt agttcagttg gttagaatgc cagcctgtca cgctggaggt cgagggttcg
      541 agccccttcc aggtcgccaa aataaaaatg ctggtgtggc tcaacggtag agcagctgac
      601 ttgtaatcag caggttgtag gttcgattcc tatcaccagc tccatgtaaa actaatacgg
      661 aggatttccc gagcggccaa agggggcaga ctgtaaatct gttgtcatcg acttcggtgg
      721 ttcgaatcca ccatcctcca ccaattaaat atgcgggtgt agctcaatgg tagagttctg
      781 gccttccaag ccagctgtga gggttcgatc cccttcaccc gctccaaaga aagcgtgggt
      841 gcatagctca gctggataga gcaacgccct tctaaggcgt gtgtccgggg ttcgaatccc
      901 tgtgcgctca ccacatatat attggggatt agccaagtcg gtaaggcaca tgactttgac
      961 tcatgtatgc gtaggttcga gtcctgcatc cccagccaaa taaaaatatg gtctattagc
     1021 tcagtcggta gagcacctga cttttaatca gggtgtcccg cgttcgagtc gcggatagat
     1081 caccaatgga gaggtgtccg agtggtttaa ggagctggtc ttgaaaacca gtgacttcga
     1141 aaggggccgt gggttcgaat cccaccctct ccgccaaaaa aatgactgaa tttggagaag
     1201 tactcaagtg gctcaagagg atcccctgct aagggattag gctgggtaac cggtgcgagg
     1261 gttcgaatcc ctccttctcc gccattcaaa aacgaggtgt agcgcagttt ggtagcgcac
     1321 atggtttggg accatggggc cgggggttcg agtcccttca cctcgaccaa cttaagcttt
     1381 tagttcagtt ggttagagtg cccaactcat ggctagtaat tctagggttt gagtccatga
     1441 aggttattct tcataggggt gtagctcagt tggtagagcg tcggtctcca aaaccgtgcg
     1501 ccgggggttc gagtccctcc acccctgcca aataaaaagg acttgaaaaa agttggattt
     1561 tagagtagta taagatagta aataagattt cgaggtgtag cgcagtttgg tagcgcacat
     1621 ggtttgggac catggggccg ggggttcgag tcccttcacc tcgaccaaga aaagggccta
     1681 tagctcaggt ggttagagcg cacgcctgat aagcgtgagg tcgctggttc gagtccagct
     1741 aggcccacca tttgcccaga tagctcagtc ggtagagcag gggactgaaa atccccgtgt
     1801 cggtggttcg attccgcctc tgggcaccag gaatacggcg gtatagctta gttggctaga
     1861 gcgttcggtt catacccgaa aggtcatagg ttcgactcct attaccgcta ccatctaact
     1921 atatca
//
LOCUS       DAELLV010000367         1891 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.369, whole genome shotgun sequence.
ACCESSION   DAELLV010000367 DAELLV010000000
VERSION     DAELLV010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.369"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(123..1496)
                     /locus_tag="KPD58_003624"
     CDS             complement(123..1496)
                     /locus_tag="KPD58_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0267328.1"
                     /translation="MVLTGAHYIYILFMVIILITMIMKKDTIVPCILGVFFMGLFFEK
                     NIFGAITAVFNSFIISLNELGPIILIIAIMVALSKALEANNAIQYMVRPFSRVIKNSN
                     TAFFVTGFVMLVLSWFFWPTPAVALVGAVFLPVAMRAGLPAIGVAVALNLFGHGLALS
                     TDFVIQGAPSITAGAAGVAVSYVINDGMILFWVMGIVTISMAFYTLKRDINKGMFREE
                     LKGFESEEVEEFNGKSKIATILVALGFLADIIAMYIFDLKGGDASALLGGTAVFLIII
                     INVINFGKDSLENVCENIIDGFVFGIKIFGAIIPIAAFFYMGEVAPLTGVFGKVLAPG
                     SQGLLSDIGIALSQTIPLNKFAVSGIETVVGAITGLDGSGFSGISLVGSLASVFGTAI
                     NASVGALAALGQISGIWVGGGCLVPWGLISAAAICGVSPIELAKRNFVPVITGLVVTT
                     IVAMFII"
     gene            complement(1683..>1891)
                     /locus_tag="KPD58_003625"
     CDS             complement(1683..>1891)
                     /locus_tag="KPD58_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG0267329.1"
                     /translation="IVFLIKVEDSKLLIIQKNRVSNFSKNVESFGIGIKNCEKIVAYH
                     NGSINVNSDDKEFAIEISIPAKES"
BASE COUNT          692 a          367 c          217 g          615 t
ORIGIN      
        1 aagaatacta ataaaaatat caataaaaat agatgtatgg cttagaatta gtaaaacaat
       61 tataataaat tatagctaca catctatttt taatttatac aattaaagat ttattcgatt
      121 tattaaataa taaacattgc tactatggtt gttacaacca atccggttat aactgggaca
      181 aagtttcgct ttgccaattc tattgggcta actccacaaa ttgccgctgc tgaaataaga
      241 ccccaaggaa ctagacagcc tccacctacc catataccac tgatttgtcc taatgcagct
      301 agtgcaccaa cacttgcatt tatagcagtt ccaaatacag aggcaagtga accaactaat
      361 gaaatcccag aaaatccaga accatcaagc ccagttattg ctcccactac agtttctata
      421 cctgatacgg caaatttatt aagtggtatt gtttgagaaa gagctatgcc tatatctgat
      481 aataaacctt gtgaacctgg tgctaatact tttccaaata cacctgtcaa tggtgcaact
      541 tcacccatat agaaaaatgc tgctattggt ataattgctc caaatatttt tatcccaaac
      601 acaaaaccat caattatatt ctcacataca ttttcaagac tatctttgcc aaaatttata
      661 acatttataa ttattattaa aaatactgct gttccaccca aaagagctga tgcatctcct
      721 ccttttaggt caaatatata catagcaatt atatcagcaa gaaatcccag agctactaaa
      781 atagttgcta ttttagattt tccattaaac tcttctactt cttcactttc aaaacctttt
      841 agttcttctc taaacattcc tttgttaata tctcttttta atgtataaaa tgccatagat
      901 atggttacga ttcccatcac ccaaaacaaa atcataccat catttataac atatgatact
      961 gcaacacctg ctgcaccagc tgttatactt ggagctcctt gaataacaaa gtctgtagat
     1021 aaggcaagac catgaccaaa taaatttaat gctactgcaa caccaattgc tggtagccca
     1081 gctctcatag caacaggtaa aaatacagct ccaactaagg caacagctgg agttggccag
     1141 aaaaaccaag aaagaactaa cataacaaat ccagttacaa aaaatgctgt attagaattt
     1201 tttataactc tcgaaaatgg tctcaccata tactgtatag cattattggc ttctagggct
     1261 ttagataatg caaccataat agctataatc aagataattg gacccaactc atttaaagat
     1321 ataataaaag agttaaacac tgctgttata gcaccaaaaa tgtttttttc aaagaacaac
     1381 cccataaaaa aaacacctaa tatacatggt actattgtat cctttttcat aatcatagta
     1441 attaatataa ttaccataaa tagaatgtaa atataatgtg ctcctgtcaa aaccatatta
     1501 ttatgtcccc tttttttaag ataataatat tatatggtct tataagtaaa cttgttactt
     1561 gcctagaggt acacaatatt gaacatccca taactaaagt cacatgcttc ctgcttcata
     1621 gaattttaca taagcaatta cacaatatat aatagcaatt acacaatata taatttaaat
     1681 ttttatgatt cttttgctgg aatagatatt tcaatagcaa attctttatc atctgaattt
     1741 acatttatgc ttccattatg atatgcaact attttctcac aattctttat accaattcca
     1801 aaactttcta cattcttaga aaaattactt actctatttt tttgtataat tagtaattta
     1861 gaatcctcta ctttaattaa aaatactata g
//
LOCUS       DAELLV010000368         1883 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.370, whole genome shotgun sequence.
ACCESSION   DAELLV010000368 DAELLV010000000
VERSION     DAELLV010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.370"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            403..741
                     /locus_tag="KPD58_003626"
     CDS             403..741
                     /locus_tag="KPD58_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0267330.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
     gene            763..1080
                     /locus_tag="KPD58_003627"
     CDS             763..1080
                     /locus_tag="KPD58_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0267331.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            1230..>1883
                     /locus_tag="KPD58_003628"
     CDS             1230..>1883
                     /locus_tag="KPD58_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0267332.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     "
BASE COUNT          826 a          180 c          267 g          610 t
ORIGIN      
        1 atttaaaaat gtttacctta ccaatataat gattaaactc aaaagtctta ttgaaaaatt
       61 ttctatttac ataaattgtt caatatataa aatagaaaat ttttttaatt ttcaaaatat
      121 attctacata tctaatatgt aattacaata aaaaatgcta taattaacat attattaatt
      181 cgttttatta ttgtagtatg gctatgaaat tatgaaaata tttctctaat taaaaatatg
      241 atttatccat atttgtttat tgtagataca gactatacaa aaataaatag cacttatttc
      301 agtataaaaa catatatagt ctatatttag actaaaaaag tataaatagt ctatataaaa
      361 caacttaaac tatgaatata atctaaagtg gagggataat aattggcaaa tataggaaaa
      421 ataataggag atagaataaa agtctataga acaaaattgg gatatagtca agagtttcta
      481 gcagaaaagg caggtttaca tccaacatat attggtcaga ttgaaagagc tgagacaaat
      541 acatctatca atattataat gaaaattgct actgctttag acataccact tgaattgtta
      601 tttgcaaata ttattacaac agaaggcgtt gataactctg tacctttaga atgttatgaa
      661 atgattaata aattaactga aaaagaacaa attgcaatgc tagagttaat aaagtgcatc
      721 attaaataca aagaaatata agtgagggaa atagtaacaa taatgaatta tataggtaaa
      781 agacttaaag aagaacggaa aaaagcaaat ttaacttcaa aagaatttgc aaatatggtt
      841 ggagtatcgc cttggtatat aactcaaatc gaaagtggta aaaagaatcc aagtcttaaa
      901 acattcataa aatttgtaaa tatattaaat atttctgcag atgttcttat aaaagatatt
      961 acttccactg gaaaaacata tttagaaaat gatataaatg aagagttaaa agacttaaat
     1021 tcaagagaac taaatctgat atcacagatt atagacatca taaaaaagaa taaaatataa
     1081 ttctttatat tttatataaa aaaaaaggtt atttgaaata aataaccttt ttttgtcttt
     1141 ttgtgttaac attgtcttat agtgctaatg agtactatta gaccatgttc tatggcctta
     1201 ttttaatata aaacgattgg agataggcta tgattgtgat tgaaggtagc gataaattta
     1261 agattgcaaa agaatatatt gatgtagaat atactctttt tagcaaagta acttttaggt
     1321 atgaaaagtt gaaatttaaa gataatgctg aattggaaaa aattaagatg tttaaatata
     1381 aaaatggcta catccctaat aaattaaacc tttcttttgg atatggattc tcttcttata
     1441 aaaagcaaat aattagagaa actgtagata ctttaagatt gacagaaatt ttttcaagcg
     1501 agaacataga agatattaaa tttataaaag atggtacaaa aaaattagaa attagcatag
     1561 agaaagttgt gaaatttaaa cgtcgaaaaa aaagaaatta tgtttgttgc tattgccctg
     1621 atatgtatag ggacataaaa ctcgacaaag aatctatcaa taagatatac aacagaaaaa
     1681 taaaaataga aagagaagtt aatatttttg aagatgagga tgttataata aacaaaaaag
     1741 tattgaagtt tccaaagtct tggacaaaga atatgcaaaa atattggtta agtgaaaata
     1801 agtatcccat acattctact gtaattgatg atgatagata taaatgttgt aatgtacaat
     1861 atacaaaaaa tagagtgata ata
//
LOCUS       DAELLV010000369         1882 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.371, whole genome shotgun sequence.
ACCESSION   DAELLV010000369 DAELLV010000000
VERSION     DAELLV010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.371"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(196..444)
                     /locus_tag="KPD58_003629"
     CDS             complement(196..444)
                     /locus_tag="KPD58_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG0267333.1"
                     /translation="MQKTKAWGIIEPEKPESQQQMAHMDFAVNDLEEAVQYAIHCGAT
                     IAEEQFTDDWRVMIDPAGHPFCLCQMKSIMESSHFSLL"
     gene            complement(475..936)
                     /locus_tag="KPD58_003630"
     CDS             complement(475..936)
                     /locus_tag="KPD58_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HBG0267334.1"
                     /translation="MKFGGVLLIVKDINKSKKFYEDVMEQKITLDLGEHVSFENGFGL
                     QSNYKAIIGYDLPIYQQSNNFQLYFEVNDIKQWESKINRIGNIEFLHYIKEYPWGQRT
                     FRFYDFDKNIIEISESMESVIKRFLKQGLALEEISKRTMYPVEFITQFQQL"
     gene            complement(974..1807)
                     /locus_tag="KPD58_003631"
     CDS             complement(974..1807)
                     /locus_tag="KPD58_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0267335.1"
                     /translation="MLKKESIHFINKNRSLIVCEDNFVYLLPCIQLRNWISNFTISFP
                     NQTIISDNYTIIPHGSVTLVFFYDATGLHSLLFGPTTKPKTVGNIANRCDVILIIEFQ
                     PAGFFPLIGIQQSELIDKVVPFSIINTSLDLEIKKIFNESLSIDKLILKLEELLISNI
                     KIEYSYEFILAIQLIIQNSGNISSQEISKKVFYSSRHLNRLFNQYLGLSMKSFSRLVR
                     INKSIKLLNNNKTSLMSICNELGFYDIPHFIKDFKIVCGITPQKYRANMSDFYSEIAK
                     F"
BASE COUNT          670 a          276 c          211 g          725 t
ORIGIN      
        1 caattcatat atgcaaaatt tatatacttt aaaatatcta agaaattatt caataaaaaa
       61 atactatttt ttaaaaatta tcattattga taaagctcta ctatttatat tttttagtgt
      121 attcatgtat aataagtaaa agcacaacgg tatatactgt tgtgcttttt tgtaattaat
      181 aaacaatatt ttggtctata acaatgaaaa atggctactt tccataattg atttcatctg
      241 acataagcaa aaagggtgtc cagctgggtc aatcataact ctccagtcat cagtaaattg
      301 ctcctctgcg attgtagctc cacaatgaat tgcatattga actgcttctt ctaaatcatt
      361 aacagcgaaa tccatatgag ccatttgctg ttgagattca ggtttttctg gttctattat
      421 gccccaagcc tttgtttttt gcaatataaa gaaaaaatta tatattttaa tattttataa
      481 ttgttgaaat tgtgtaataa attcaactgg atacatagtg cgttttgaaa tttcttctaa
      541 agctaaacct tgttttaaaa acctctttat gacgctttcc atactttctg aaatttctat
      601 aatattttta tcaaaatcat aaaacctaaa agttctttga ccccatgggt attcttttat
      661 ataatgcaaa aattcaatat ttcctattct atttatctta ctttcccatt gtttgatgtc
      721 attaacctca aaatacaact gaaagttatt tgattgttga tatattggta aatcatatcc
      781 aattatagct ttataattac tttgtaatcc aaatccattt tcaaatgata catgttctcc
      841 taaatctaat gtgatttttt gctccataac atcttcataa aacttttttg atttatttat
      901 gtctttgaca attagcaata ctcctccaaa tttcatctta tcactatcct ttctttgtta
      961 taagtattat aacttaaaat tttgcaattt cactgtaaaa atcagacata ttagctcggt
     1021 atttttgagg agttattcca cagacaattt taaaatcttt aataaaatgt ggtatatcat
     1081 agaatcctaa ttcattacat atagacatca atgaagtttt attattgttt agaagtttta
     1141 tagatttatt aatacgtacc aaacgagaaa atgacttcat actcaaccct aggtactgat
     1201 taaagagtct gtttaaatgc ctagaactat aaaatacttt tttagaaatt tcttgtgaag
     1261 atatatttcc agagttttga ataatcaact gaattgccaa aataaactca tatgagtatt
     1321 caattttaat attagaaatc aataattctt ccaactttaa aatcaattta tctatagata
     1381 aagattcatt aaatattttt ttaatttcta aatctaatga agtattaatt atagaaaatg
     1441 gaacaacttt atcaataagt tcactttgct gtatacctat aagcgggaaa aaaccagcag
     1501 gttgaaattc tataattagt ataacatcac atctattagc aatatttcct actgtttttg
     1561 gtttagtagt tggaccaaat aatagactat gtaaccctgt ggcatcataa aaaaacacca
     1621 aagttacact tccatgagga ataatcgtgt aattatcaga aattattgtt tgatttggaa
     1681 acgatatagt aaaattcgat atccaatttc ttaattgtat acaaggcaag agataaacaa
     1741 aattgtcttc acaaactata agtgacctat ttttatttat aaaatgaata ctttcttttt
     1801 ttaacatata ttatatcctg tcctatcgtg aatatttatt aaagtaagaa gaatccatat
     1861 tatctctata tatcattaac tg
//
LOCUS       DAELLV010000370         1845 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.372, whole genome shotgun sequence.
ACCESSION   DAELLV010000370 DAELLV010000000
VERSION     DAELLV010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.372"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            221..>1845
                     /locus_tag="KPD58_003632"
     CDS             221..>1845
                     /locus_tag="KPD58_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HBG0267336.1"
                     /translation="MKIDMIYKLIRRNTEPSRWSRGVKYYTHQLVDYIDIDIDDLQNN
                     LLTFRGGVNSEDNYSFYFNSITIDIKHFKIIENDCSCADCQSKGKSIFICKHIVAIVI
                     KSLKQIKNKKDVPPILIDILGADKDKLSNTNSKTSLDLSKQLLSLLDNTKRERVNLDV
                     LINVDSNKAIAEFKIGIDKMYILKNITEFAYSKLNGYELDFGKKFTYNPNIHYFADED
                     EKLVDIIEEYGTNLSYNFSSSHSKKFMEINASSLKQFLNAIKYKDFTLIFSKEEYHPK
                     ILNDTLPVRFDFKNIEDKIVLYSKDSLPVPLTKKNDVLLYDGNIYLLSHKKAHDYSKI
                     YQILSKTKELKFDKEDSKDVLGLLVPRLKSISQEVHLDDNIKNNITKDFKSEFYFDMI
                     DGNICCDVKYIYDDEDKKFVLPNIQKEATIENKLTSSQFTKENNHYVFKGTDHDLFTF
                     LDLQLESLKEFGDIYYSEKFKLKKIYNASSIQASINQTNQNYLEFNFNISDINPKEYK
                     DILKAFQEKRTFYKLKDGNFIDLSERETKDFFE"
BASE COUNT          760 a          200 c          248 g          637 t
ORIGIN      
        1 aataataaga ttttatattt attaataatc tatttatata attatttttc attcatagtt
       61 tataagataa ttcaataaaa atctaatagt aaattgttat ttacacatat attgtgatag
      121 ataaaagcta ttcatatctg ccttaatttt agtcttaaat ttagagtgta aattgaatgc
      181 atttagatta taatatatac agtgaggtaa ggtgaataat atgaaaatag atatgatata
      241 taaactaata agaagaaata cagaaccatc aagatggtca agaggtgtaa aatattatac
      301 tcatcaatta gttgattata tagacataga tattgatgac ctacaaaata acctgttgac
      361 atttcgtggt ggagtcaatt ctgaagataa ttatagcttt tattttaatt caataactat
      421 agatataaag cactttaaga taattgaaaa tgattgtagt tgcgctgact gtcaatcaaa
      481 gggcaaaagt atttttatat gtaagcatat agttgctatt gtgataaaaa gcttaaaaca
      541 aataaaaaat aaaaaagatg ttcctcctat tctaatagat atattaggtg cagataagga
      601 taagctttct aatacaaatt ctaaaaccag cttagattta agcaagcaat tactttcttt
      661 gttagacaat actaaaagag aaagggttaa tttagatgta ttgataaatg tggattcaaa
      721 taaagcaatt gctgaattta aaataggaat tgataaaatg tacattttaa aaaatattac
      781 tgaatttgca tattcaaaac ttaatggata tgaattagat tttgggaaga aatttactta
      841 caaccccaac attcattatt ttgctgatga agatgagaag ttagtagata ttatagaaga
      901 atatgggaca aatctttcat acaacttttc ttcatcacat tcaaaaaaat ttatggagat
      961 aaatgcaagc tctttaaaac aatttttaaa tgctattaag tacaaggatt ttacccttat
     1021 tttttctaaa gaggaatatc atccaaagat attaaatgat actctacctg taaggtttga
     1081 ttttaaaaat atagaagata aaatagtctt atattctaaa gattcactac ctgttccact
     1141 tactaaaaaa aatgatgtac ttttatatga tggcaatatc tatttattat cacataaaaa
     1201 agctcatgat tattcaaaaa tatatcaaat attatcaaag actaaagagt taaaatttga
     1261 taaagaggat tctaaggatg ttttaggatt gctagttcct agattaaaat ctatatctca
     1321 agaagttcat ttagatgata acataaaaaa taatataaca aaagatttta aatctgagtt
     1381 ctactttgat atgattgatg gcaacatatg ttgtgatgta aagtatatat atgatgatga
     1441 agataaaaaa tttgtgctcc ccaacataca aaaggaagct actattgaaa ataaacttac
     1501 ttcttcacaa tttacaaagg aaaataatca ttatgtgttt aaaggaactg accatgacct
     1561 atttacattt ttagatttac aattagaatc tctaaaggaa tttggagata tatattattc
     1621 tgagaagttt aaattgaaaa aaatatataa cgcttcatca attcaagctt caatcaatca
     1681 gactaaccag aattacctag aatttaattt taatatatct gatataaatc caaaagagta
     1741 caaggatatt ttgaaagctt ttcaagaaaa acgtactttt tataaactta aagatggaaa
     1801 ctttatagat ttatcagaaa gagaaacaaa agattttttt gaatt
//
LOCUS       DAELLV010000371         1843 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.373, whole genome shotgun sequence.
ACCESSION   DAELLV010000371 DAELLV010000000
VERSION     DAELLV010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.373"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            103..1548
                     /locus_tag="KPD58_003633"
     CDS             103..1548
                     /locus_tag="KPD58_003633"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBG0267337.1"
                     /translation="MIDNKYTTYSNPLTDRYCSKDMSYIFSPQFKFSTWRRLWVALAE
                     GEHELGINITEEQLDELRANIDNINFDEARKIEKEVRHDVMSHVKAYGLQCEKAKGII
                     HLGATSAYVGDNTDVIQMNEALKLLRVKLVNLINNLKEFALKYKDMPALGFTHFQAAQ
                     LTTVGKRACLWAQDLILDLEDLNYRIDNMKLRGVKGTTGTQASFLSLFEGDHEKVKEL
                     DKKICKKMGYHSSYAVSGQTYTRKLDYQVISILSGIAQSMHKMTNDIRLLQSLKELEE
                     PFEKNQIGSSAMAYKRNPMRSERIASLSKYIISESISPAMVQATQWLERTLDDSANKR
                     LAIPQAFMAADAILEIGINVTDGLVVYENMINRRVNEELPFMATETILMEAVKRGGDR
                     QELHEIIREYSMKAAYRVKHEGKDNNLIELIMNDDSFKMSKEEILSIMDPKNFIGRAP
                     EQVVEFVNEVVEPAIKDYKEDLGKVDVDLRV"
BASE COUNT          766 a          220 c          324 g          533 t
ORIGIN      
        1 aaaaaagtct tgttatagtt cgtaaaatta tatataatta tttatgtact aatgtgtaca
       61 cagtaatcac acgacaaaag atttaaggaa ggtgctaata taatgataga taataaatat
      121 actacttact caaacccact tacagacaga tattgtagca aggacatgag ttatatattt
      181 tctccacaat ttaaattctc tacatggaga aggttatggg ttgcacttgc agaaggtgaa
      241 catgagttag gaatcaatat aactgaagaa caattagatg agctaagagc taatatagat
      301 aatataaatt ttgatgaagc tagaaaaata gaaaaagaag taagacatga tgtaatgtct
      361 cacgtaaaag cttatggttt acaatgtgaa aaagccaaag gaataataca tttaggagca
      421 acatctgcat atgttggaga taatacagat gttatacaaa tgaatgaagc tttaaaactt
      481 ctaagagtaa agcttgtaaa tttaataaac aatttaaaag aatttgcact taagtacaaa
      541 gatatgccag ctcttggatt tacacacttc caagctgctc aacttacaac agtaggaaaa
      601 agagcttgtt tatgggctca agatttaatt cttgatttgg aagatttaaa ctatagaata
      661 gacaacatga agttaagagg agttaaaggt actactggta ctcaagcaag ttttttaagc
      721 ttatttgaag gagaccacga aaaagtaaaa gagttagata agaaaatttg taaaaagatg
      781 ggataccatt cttcctatgc agtaagtgga cagacatata ctagaaagtt agattatcaa
      841 gtaataagca tactatcagg gatagctcaa agtatgcata agatgactaa tgatataaga
      901 cttttacaaa gtttaaaaga attagaagag ccatttgaaa aaaatcaaat aggttcatca
      961 gcaatggctt ataagaggaa tccaatgaga agtgagagaa tagcatcttt atctaagtat
     1021 ataatctctg aatctataag ccctgcaatg gtacaagcca ctcaatggtt agagagaaca
     1081 ttagatgact cagccaataa gagattggca atacctcaag catttatggc agcagatgct
     1141 atacttgaaa tagggataaa tgtaactgat ggtttagtag tatatgaaaa tatgataaat
     1201 agacgtgtaa atgaagaact tccttttatg gctacagaaa caatacttat ggaagctgta
     1261 aaaagaggtg gagatagaca ggaattgcat gaaataataa gagaatattc tatgaaagct
     1321 gcctatagag taaagcatga aggcaaggat aataacttaa ttgaacttat aatgaatgat
     1381 gattcattta aaatgagcaa ggaagaaata ctatctataa tggacccaaa aaactttata
     1441 ggtagagctc cagaacaagt tgtagaattt gtaaatgaag ttgtagaacc tgctataaaa
     1501 gattataagg aagacttagg aaaagtagat gtagacctaa gagtatagaa ttgtacagat
     1561 aaaatataat taaaaacggc tatcttattt ataaataaga tagccatttt aatgggctag
     1621 cgtcttctct ccaccgctgt gaagagttct tctttactta ctataccatg aatttaataa
     1681 aatagctata taaataatat ttaataggaa taatataaat aaaaataaaa aaatgataag
     1741 attactaatt aaatttaaac attcagcaaa aactattgta cttgcaatat ttttaatgta
     1801 aactgaaata aagttattta gataatttaa taaattttaa aac
//
LOCUS       DAELLV010000372         1840 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.374, whole genome shotgun sequence.
ACCESSION   DAELLV010000372 DAELLV010000000
VERSION     DAELLV010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.374"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            347..>1840
                     /locus_tag="KPD58_003634"
     CDS             347..>1840
                     /locus_tag="KPD58_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0267338.1"
                     /translation="MRDEILSFVNECVLFNNFDELKKESGIITEDIVKKFNLSRTQSS
                     RLLNDLVKSDNLIKINSRPVIFLPKNKINDVVGPTKKSIYENLDELKNECKLLKQETV
                     FNSIIGYENSLKEVIEQIKTAVLYPNGGLTIMLTGESGVGKTFLAKIIYKYSVSSNVL
                     EKNSPYNVLNCAQYYNNLELLSSLLFGHVKGAYTGANESRAGLIEQSDGGVLFLDEVH
                     RLNSEGQEKLFTFMDTGTFSRIGENGVIRKARVRLIFATTESLSEFLQTFLRRIPINI
                     YVPNIEERGTIEKRKIVEHFIYEESKVLGLPIEITQITLNAILKIKFKGNIGECKNVI
                     KYACGRAYAKNQRRNGNIFVTLQDLPRNLYIENVDIFQFQSGKSKSIIFSPKQFSIEM
                     SNGRNKRNIIYQSFIKCLDYFDRLEKNEITKEKFSKKSSNLMIRLMDNLVFSNNDENN
                     NVLMELIISIMQDIFRNLEVNNNIKYDGNNVLAFSSYLYLKDESYQLS"
BASE COUNT          770 a          167 c          292 g          611 t
ORIGIN      
        1 taaaaataaa tttatttata gcctttttaa atttgaaata atattcataa ggtaataaaa
       61 gttacaaata ttttcttcgc taataagttt taaaatacct ataatgtttt actttttatt
      121 ataataaata caataaatat attcttatat aaattctatg tcagatatat ggaaagataa
      181 gaaccttgag attatgttaa aaaatctaga ttttatactt ataaaaagtt tgattttaca
      241 accttacaaa agagaatcaa gataggaata taattatctt tttcataaat atataaatga
      301 tatactaata ttatagtatt attgatattt gtattggagg ttgaacatga gggatgaaat
      361 tttaagtttt gtaaatgaat gtgtattatt taacaacttt gatgaactta aaaaagaaag
      421 tggcataata acagaagata tagtaaaaaa gtttaattta agtagaactc aatcaagtag
      481 attattaaat gatttagtaa aatctgataa tcttataaag ataaacagta gacctgttat
      541 ttttttacct aaaaataaaa taaatgatgt agtaggacct acaaaaaaaa gtatttatga
      601 aaatttagat gaacttaaaa atgagtgtaa attattaaag caagagactg tgtttaatag
      661 tatcatagga tatgagaata gcttaaaaga agtaatcgaa caaataaaaa ccgctgtact
      721 ttatccaaat ggcggactta caataatgct gacaggtgaa agtggagtag gaaaaacttt
      781 tttagccaag ataatataca aatatagtgt aagcagtaat gtattagaaa aaaattctcc
      841 atacaatgtg ttaaactgtg cgcagtatta taataatttg gagttattgt ctagtctatt
      901 atttgggcat gtaaaaggag catatactgg agcaaatgaa tctagagcag gtctaataga
      961 acaatcagat ggtggtgttt tatttttaga tgaagtacat agattaaata gtgaaggtca
     1021 ggaaaagtta tttacattta tggatacagg tacgttttct aggattggag agaatggagt
     1081 aattagaaaa gctagggtaa gattaatatt tgctacaaca gaaagtctat ctgaattttt
     1141 acaaaccttt ttaagaagaa tacccataaa tatttatgtt cccaatatag aagaaagagg
     1201 gactattgaa aaaagaaaga ttgtagaaca ttttatttat gaagaaagta aggtacttgg
     1261 tttgcctata gagataactc aaattacatt aaatgcgatt ttaaagataa aattcaaggg
     1321 gaatataggt gaatgtaaaa atgtaataaa atatgcatgt ggacgagctt atgcaaaaaa
     1381 tcaaagaaga aatggaaata tttttgttac tcttcaagac cttcctagaa atctttatat
     1441 agaaaatgta gatatatttc aattccaaag tggtaaaagc aaaagcatta tcttttctcc
     1501 taaacaattt agtattgaaa tgtcaaatgg aagaaataaa agaaatataa tctatcaaag
     1561 tttcataaaa tgtttagatt actttgatag actagaaaaa aatgaaataa caaaggaaaa
     1621 atttagtaaa aagagttcta atctaatgat tagactaatg gacaatttag tcttttcaaa
     1681 taatgatgaa aataataacg tacttatgga gcttataata tcgataatgc aggatatatt
     1741 tagaaattta gaagttaata acaatataaa atatgatggg aataatgtat tggcattttc
     1801 gtcttatctt tatttgaagg acgaatctta tcaattgtct
//
LOCUS       DAELLV010000373         1838 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.375, whole genome shotgun sequence.
ACCESSION   DAELLV010000373 DAELLV010000000
VERSION     DAELLV010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.375"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            116..1558
                     /locus_tag="KPD58_003635"
     CDS             116..1558
                     /locus_tag="KPD58_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG0267339.1"
                     /translation="MRTIRRVLALGLTLAIFLINVPNVDALTSDTIKGNNIYETAGLI
                     ADKKSYDTAIMVNMDNSIADGLSASGLAGAVDAPILLAQKNKIPNETKQRLKNVKKIY
                     IIGKELSISKSVETELKNTGAQVTRLGGDDRIKTSYSVAKEVNGIKKVDEVILTNAYK
                     GEADTISAAPVSVRDIAPIVLTDGKSVPFSTSNVKTYAVGGSISMSTSLVNKTNAKRL
                     GGSDRYDTNKKVIKEFYPDASEFYLSDGYDLVNALTGSTIAKENPIVLVSESSDKSIL
                     AGADKITRLGSISDSVYNKCVSAAQNNGDSSTKGESPMKNETSILGQPTASLEACLKW
                     AKSKKANDLFIELIPILYDTAVQEGVNPVLAVAQSAKETGFCNFGGVLDASFKNPCGL
                     KTSVGGSDTDKNAHSRFDTWEEGILAQIQHLCLYAGQDGYPLSNPVDPRHEKSLFGKA
                     KTVESLSNNWAGGQYGQDLVRMMGEIEATK"
BASE COUNT          695 a          207 c          352 g          584 t
ORIGIN      
        1 tatgatataa taacaaatat ataattatta aattataata aatgactata gcgtagatgt
       61 aatactatgt tatagtcatt tttgaattaa acttgatata tcaaggggga agaatatgag
      121 aacaattaga agagttttag cattaggttt aacattagct atatttctaa taaatgtacc
      181 taatgtagat gcattaacat cagatacaat taaaggtaac aatatatatg agacggctgg
      241 attaatagca gataaaaaga gttatgatac agcaattatg gtaaatatgg ataattctat
      301 agcagatggt ctttcagcaa gtggtcttgc aggtgctgtt gatgctccaa ttttgcttgc
      361 acaaaaaaat aagataccaa atgaaacaaa acaaagatta aaaaatgtta aaaagatata
      421 cataataggt aaagaattat caataagtaa gtcagtagaa actgaactaa aaaacacagg
      481 ggcacaggta actagattag gtggagatga cagaataaag actagttata gtgttgctaa
      541 agaggtaaat ggtatcaaaa aagttgatga agtgatatta actaatgcat ataaaggaga
      601 agcagataca ataagtgctg cacctgtatc agtaagagat atagctccta tagtacttac
      661 agatggaaag agtgtgcctt tttcaacaag caatgtaaaa acatatgcag taggtgggag
      721 tatatcaatg agcactagtt tagttaataa aactaatgct aaaagacttg gtggttcaga
      781 tagatatgat acaaataaga aagttataaa agagttttat ccagatgcat cagaatttta
      841 tttgagtgat ggatatgatt tagtaaatgc acttacaggt tctacaattg ctaaggaaaa
      901 tccaattgta ttggtgtcag aaagtagtga taagtctata ttagcaggag cagataagat
      961 tactagatta ggttcaataa gtgacagtgt gtataataag tgtgtttctg ctgcacaaaa
     1021 taatggcgat tcgtctacaa aaggtgagtc acctatgaaa aatgagacaa gcatattagg
     1081 tcagccaaca gctagtttag aagcgtgctt aaaatgggca aaatctaaaa aagctaatga
     1141 tttatttata gagttaatac caatattata tgatactgct gttcaggaag gtgttaatcc
     1201 tgttttggca gtagctcaat ctgcaaaaga aactggtttt tgtaattttg gtggagtatt
     1261 agatgcatca tttaagaatc cttgtggact taaaacttct gtaggtggtt ctgatactga
     1321 taagaatgcc cattcaagat ttgatacttg ggaagaagga atattagctc aaattcaaca
     1381 tttatgctta tatgcaggac aagatggtta cccactttca aatccagtag accctagaca
     1441 tgaaaaatct ttatttggta aggctaaaac agttgaaagt ttatctaata attgggctgg
     1501 aggtcaatat ggacaagatt tagtaagaat gatgggagaa attgaggcaa caaaataatt
     1561 gaagattatc aaatatccaa taaataagga ttgattatta tgaaaaagag taatattttt
     1621 agtatcatat ttcctacagt cataatggtg ctaatgtttt taacttttgc tacagatata
     1681 tttaatattc cagatattca atctaaaagt atttttgttg tgggaatggt tttgattttt
     1741 ccaatctctt ttttgataca agggataatt tgtgtattaa ataagactaa ttggatttta
     1801 tctcttattg tgtctttagt aacttatata gtattaat
//
LOCUS       DAELLV010000374         1816 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.376, whole genome shotgun sequence.
ACCESSION   DAELLV010000374 DAELLV010000000
VERSION     DAELLV010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.376"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            218..952
                     /locus_tag="KPD58_003636"
     CDS             218..952
                     /locus_tag="KPD58_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0267340.1"
                     /translation="MNLAKGLDVLKISSNVFGEDKVMYIPVIYTEDDATLIDTGLPGQ
                     GDLIIDALNKSNTSFDRLKNIIITHHDIDHIGNINYLREKSKNNIKVYAYKSEISYIT
                     GEETPFKLYMLEQMVDKIDDKMLSMLNVMGLGFKSSYTKVDVSLDNHEKLNLGEEIEV
                     IHTGGHTRGHICLYLKESKVLIAGDLLQVENGELKPVDVMYSNKQELKDAIKNISNYD
                     IETIVFSHGGLYQRNIIGTLKNLIIE"
BASE COUNT          810 a          161 c          262 g          583 t
ORIGIN      
        1 aataaaagta attgcaaagc ttgcaattac ttacttctat attaattact ataccataaa
       61 ataacaaaaa aatcaagtcg agtaatcctt caacttaaat gatatactag gtattaataa
      121 tttttaatta ataaaatata actagaggaa aatctacatt ttctcaattt ctttaattaa
      181 attgacctct aattaagtaa ttataaggag aaaaattatg aatttagcaa agggattaga
      241 tgttttaaaa atttcatcaa atgtattcgg tgaagataaa gttatgtata ttcctgtaat
      301 ctacacagaa gatgatgcaa cacttattga cacaggtctt ccgggtcaag gtgatttaat
      361 aattgatgct cttaataaaa gtaacacgag ctttgatagg ttaaaaaata taataattac
      421 tcatcatgat atagaccata taggaaacat aaattatcta agagaaaaat ctaagaataa
      481 tatcaaagta tatgcataca aaagtgaaat cagctatata acaggagaag aaactccttt
      541 taaactttat atgttggaac aaatggtaga taaaattgat gataagatgc ttagtatgct
      601 aaatgttatg ggacttggat ttaaatcatc atatactaaa gtagatgtgt cattagataa
      661 ccatgaaaaa ttgaatctag gtgaggaaat agaagttata catacaggag gtcacactag
      721 aggtcacata tgtctttatt taaaagagtc taaagtactt attgctggag atttgttaca
      781 ggtagaaaat ggagaactta agcctgttga tgtgatgtac agcaacaaac aagagttaaa
      841 agatgctatt aaaaatataa gtaattatga tatagaaacg atagttttta gtcatggtgg
      901 attatatcaa agaaacataa ttggaacatt gaaaaaccta attattgagt agtactatat
      961 gatttacaat aatttcttaa attacttaaa attatatcta aagatgtaga atcaaaatta
     1021 aatataagta ataagaattt tgctttaaaa ttatacttaa gtgttaagga tgatacgtag
     1081 tattacaagt attttagaat ataatttttt atagtaattt taaggttcta caatacttca
     1141 gttgaaaaat atttaacagc atagtataat aggaaagaaa atactttaaa taaactttag
     1201 catacaaagt gaagaaaaat atttaaatta tatataatat tgtagagtga ggtaaaatat
     1261 ttaagttata tatcataatg cagagtgaag aaaaatattt agataatgta ccataataca
     1321 aagtgagtaa aaatatttaa atcatgtatc ataatgcaaa gcgaagaaaa atatttaaat
     1381 aatgtaccat aatgtagagt gagtaaaaat atttaaataa tgtgttataa tacaaagcga
     1441 ataagattat ttaaagcatg tgttataata caaagcgaat aagattattt aaagcatgta
     1501 ttataatata aagtgagtaa aaatatttaa atcatgtatc ataatacaaa gtaaagaaga
     1561 ttatttaaac catatatcat aatgcaaagt gaagaaaaat gtttaaatta tgtatcataa
     1621 tataagcaga gtgaagaaaa atatttaaat aatatatcat aatgtagagt aaagaaaaaa
     1681 tttaaatcat gtatcataat gcaaagtgaa gaaaaatgtt taaattatgt attataatat
     1741 aagcagagtg aagaaaaaat ttaaatcatg catcataata taaacagagt gaagaaaaat
     1801 atttaaataa tatatc
//
LOCUS       DAELLV010000375         1813 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.377, whole genome shotgun sequence.
ACCESSION   DAELLV010000375 DAELLV010000000
VERSION     DAELLV010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.377"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(299..1627)
                     /locus_tag="KPD58_003637"
     CDS             complement(299..1627)
                     /locus_tag="KPD58_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG0267341.1"
                     /translation="MGSNELKKTLGVSAALSTVVGSVIGAGVFFKPQAVYTLTGGAPG
                     LGILAWLIAGIITITAGLTAAEVSVAIPKTGGMMVYIKEIYGEKLGFLTGWMQIVLFY
                     PGMMAALGVIFGEQASALIGSPSLLLPIAIGIIVIVAGLNMLGSKTGGVIQTVSTICK
                     LIPLILIMVVGFIKGGGNNPILTPMVGEGLSLGSVLGQVLIAILFAFDGWMNVGTLAG
                     EMKNPGKDLPKAIIGGLSVVMAVYFIINLAYLWVLPANELANYASPASAVAEVIFGSM
                     GGKIISVGILISVFGALNGFLLTGSRVAYTLATDKTLPKYGIFSKLNSAQVPANAIAL
                     VSVIASIYALSGQFNLLTDLAVFATWIFYVLTFIGVMKLRKTHPNIPREYKVPLYPIV
                     PIIAIASGIFVVVNQLCFAGMKTTMISIGGLVITAIGLPVYAYMTRGVKR"
BASE COUNT          663 a          328 c          207 g          615 t
ORIGIN      
        1 aaagatttaa ttttcataaa agctatgtat aatatttgtt gtacagatac cacttagttt
       61 gataaaaaac actttaaaat aaagtatttt aaaataaata attttttaga tgtttaattt
      121 tataatcaaa aaactaaaaa taaaatgaat accttaaaat aaacttttta gttcatggaa
      181 cgctcttttt ttgtaaaatc aaacatatct ttaccatttt aacaataaaa aagagactta
      241 tctctatttg agacagtctc ttttatttta attatgaagc atttgtaaac tacttggttt
      301 atctttttac tcctctagtc atataagcat acactggtaa gcctatagct gttattacaa
      361 gacctcctat agatatcata gtagttttca tacctgcaaa acacaattga ttaactacaa
      421 caaatatacc acttgctata gctattatag gaacaatagg atataatgga actttatact
      481 ctcttggtat atttggatga gtctttctaa gtttcataac ccctataaaa gttaatacat
      541 agaatatcca agtagcaaat acagctaagt cagttaataa attaaattga cctgacaaag
      601 catatataga tgcaataaca gatactaatg ctattgcatt tgctggaact tgagcagaat
      661 ttaatttaga aaatatacca tactttggta aagtcttatc tgtagctaaa gtatatgcca
      721 cccttgaacc agttagtaaa aatccattta atgctccaaa cactgaaatt aatataccta
      781 cagatattat ttttccaccc attgaaccaa atataacttc agcaactgct gatgcaggag
      841 aagcataatt tgctaattca ttagctggta atacccaaag atatgctaaa tttattataa
      901 aataaacagc cataacaact gacaacccac ctattatagc ttttggaagg tcttttccag
      961 gattcttcat ttctccagct aaagtaccaa cattcatcca tccatcaaaa gcaaataata
     1021 tagcaattaa aacttgacct agtacactac ccaggcttaa tccttcacca accataggtg
     1081 taagtattgg attatttcca ccacctttaa taaatccaac aaccattatt aaaataagtg
     1141 gtattaactt acaaattgta gaaacagttt gtatgactcc tccagttttt gaacctaaca
     1201 tattaagccc tgcaactatt actataattc caattgctat tggaagtaaa agtgatggac
     1261 taccaattag tgctgatgct tgttctccaa atataacacc taatgctgcc atcatccctg
     1321 gataaaataa tacaatttgc atccatccag ttaaaaaacc taatttttct ccataaattt
     1381 cttttatata taccatcatt ccaccagttt ttggtatcgc aactgatacc tctgcagctg
     1441 taagaccagc tgttatagtt atgattccag ctattaacca tgccaatata ccaagacctg
     1501 gagctcctcc agttaatgta tagactgctt gtggtttaaa aaatacgccc gctcctataa
     1561 cggaaccaac aacagttgat aatgctgctg atacgccaag agtctttttt agttcattac
     1621 ttcccatttt tttcctcctc attaatccta tttaatttta atttcatagt taaataagca
     1681 tatattatgc caatatatag aaaaatagct ttattattta ttaaaataaa ataaactcta
     1741 cttagcttta gtaatctttt atttttagtt atgtttaaat ataaaaaaat tataaaaacc
     1801 aaaaatccaa ata
//
LOCUS       DAELLV010000376         1782 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.378, whole genome shotgun sequence.
ACCESSION   DAELLV010000376 DAELLV010000000
VERSION     DAELLV010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.378"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(245..1537)
                     /gene="eno"
                     /locus_tag="KPD58_003638"
     CDS             complement(245..1537)
                     /gene="eno"
                     /locus_tag="KPD58_003638"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBG0267342.1"
                     /translation="MSVIELVYAREVLDSRGNPTVEVEVVLEDGAMGRAIVPSGASTG
                     AFEAVELRDGDKGRYLGKGVETAVANVNEIIAPEIEGMDAFDQPAIDAIMIELDGTPN
                     KGKLGANAILGVSMAVARAAADEIGLPLFQYLGGVNAKQLPVPMMNILNGGEHADNNV
                     DVQEFMILPVGACCFKEGLRMGAEVFHSLKKVLGEKGLACGVGDEGGFAPNLGSNREA
                     LELIVEAITKAGYKPGEDVMLGLDVAATEMYNKETKKYVLAGEGKELTAAEMVALYED
                     WSNNFPIITIEDGLDEEDWDGWKLLTEKLGNKLQLVGDDLFVTNTERLEKGIENGVAN
                     SILVKVNQIGTITETLDAIEMAKRAGYTAVISHRSGETEDSTIADLAVAVNAGQIKTG
                     APSRTDRVAKYNQLLRIEEMVGEQARYCGLKSFYNLKK"
BASE COUNT          539 a          354 c          188 g          701 t
ORIGIN      
        1 tacttcaatg acaattatga ttaaatcaaa atatgtcttc acttttattt tttaataaaa
       61 acctcctatg caattagata aaaatgagat agaaacattt attcatattt tttctgaatc
      121 tatgaataaa attaagtaat aaaatataaa aaaggataag tgaattgaaa aatcaattaa
      181 cacttatctc ttttatatct tatattctct cttcattaat cttttttagt aaccatttta
      241 ataactattt ttttaagtta tagaaagatt ttaatccaca atatctagct tgctctccaa
      301 ccatttcttc tattcttaat aattggttgt acttagcaac tctatcagtt cttgatggag
      361 caccagtttt tatttgacca gcatttactg caacagctaa atctgctata gtagaatctt
      421 cagtttctcc tgatctgtga gatataacag cagtatatcc tgctctctta gccatttcta
      481 tagcatctaa agtttcagtt attgtaccta tttgattaac tttaactaat atagagttag
      541 ctactccatt ttcaattcct ttttctaatc tctctgtgtt agtaacaaat aagtcgtcac
      601 caactaattg taatttatta cctaattttt cagttaataa tttccatcca tcccaatctt
      661 cttcgtctaa accatcttct atagttatta ttgggaagtt gtttgaccaa tcttcatata
      721 aagcaaccat ttcagcagca gttaactctt ttccttctcc tgctaaaacg tattttttag
      781 tttctttgtt gtacatttct gtagcagcaa cgtcaagacc taacataaca tcttcacctg
      841 gcttatatcc agcttttgtt atagcttcaa ctattaattc taaagcttct ctgtttgaac
      901 ctaagtttgg agcaaatcca ccttcgtcac ctacaccaca agctaatcct ttttctccta
      961 aaactttctt taatgaatgg aatacttctg ctcccattct taaaccttct ttgaaacaac
     1021 aagctcctac tggtaatatc ataaattctt gaacatcaac gttgttatca gcatgctctc
     1081 caccgtttaa tatgttcatc attggaacag gtaattgttt agcatttact ccacctaagt
     1141 attggaataa tggtaaacct atttcatcag cagctgctct agctactgcc attgaaacac
     1201 ctaatatagc attcgctcct aatttacctt tgtttggagt tccatctaat tctatcatga
     1261 ttgcatctat agctggttgg tcaaatgcat ccattccttc tatttcaggt gctattattt
     1321 catttacgtt agcaacagct gtttctacac cttttcctaa gtatcttccc ttatctccat
     1381 ctcttaattc aacagcttca aaagctcctg tagatgctcc tgatggaact attgctcttc
     1441 ccattgctcc atcttctaat actacttcaa cttctacagt tggatttcct cttgagtcta
     1501 atacttctct agcatatact aattcaataa ctgacatttg tatatctccc ttcgattttc
     1561 tattagttaa attttcattt actgtaaaca tccgtctact attttaatct actttctata
     1621 ttatagtaca taaagttgct tttgaaaatt aggtacaaat ttatttgatg ggtaaatttg
     1681 taaaagttcc tatttttatt ttatttttta taatatttta atttatataa tataaaaaaa
     1741 cttgtctaaa atccaaaacc aatcttaaat acacaaatat ta
//
LOCUS       DAELLV010000377         1727 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.379, whole genome shotgun sequence.
ACCESSION   DAELLV010000377 DAELLV010000000
VERSION     DAELLV010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.379"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            306..1598
                     /gene="brnQ"
                     /locus_tag="KPD58_003639"
     CDS             306..1598
                     /gene="brnQ"
                     /locus_tag="KPD58_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBG0267343.1"
                     /translation="MGEKQINFKDVIVIGFALFAMFFGAGNLIFPPYLGVLSGSSWLV
                     AFIGFLFADGGLALLAVIAATKFNGDTSKMFSRAGKGLSIALGCAMVICIGPLLAIPR
                     TAATTYEMGILPTIGSGISPVIFSIVFFALVLVLTIRPSKVVDIVGSILTPALLIALA
                     VLIVKGIVSPLGEIRDSSLIQNVFAEGITQGYQTMDALAASVFASIIIMSVIAKGYTG
                     EKEKMKATISAGVIAVIGMALVYGGLCYLGATVSQQYGQDVQQTALIVAITAALLGTT
                     GKILLAIIVALACLTTAIGLSSAAGQYFSTLTDGKLKYEHIVIVVCVFSAIISNFGVS
                     TIIQFSSPILSMVYPATITLVILALFSNKIKNDNVFKCAAYMALLVSVLTVATSFGIK
                     IPLVNSLPFASLGFNWIVPVVIAGIIGNFIPSKSSKTA"
BASE COUNT          631 a          213 c          296 g          587 t
ORIGIN      
        1 ttaataataa atcgaataat ctaaaaaatc ttaatatttt aattattcgg aatagttaat
       61 tttatcaaga aaaattagag aaaaaacaaa aaaccaacta tgtttaaagt atacaggttg
      121 attttgttta attaagaaag ttgtattaaa tattgtgaag attttataaa ttatgaaaca
      181 taattgacaa atttaagtca aaaatgtata attaaataaa atctcgtcaa aaggttttca
      241 tttaattaag agattggact tattttttag taaaaggtag taaaattcaa atgggggtgc
      301 tttaaatggg tgagaaacaa atcaatttta aagatgtaat tgtaattgga tttgcactat
      361 ttgcaatgtt ttttggtgca gggaacttaa tcttccctcc atatctagga gttttatctg
      421 gaagtagttg gctagtagct ttcataggat tcctttttgc agatggtggt ttggcattat
      481 tagcagttat agcagcaact aagttcaatg gagacacatc gaagatgttc tcaagagcag
      541 gcaaaggatt aagtattgca ttaggatgtg caatggtaat ctgtataggg ccacttttag
      601 caatacctag aacagcagca acaacttatg aaatgggtat attacctaca ataggttctg
      661 gaataagccc agtaatattc tctatagtat tctttgctct agtattggtt ttaacaataa
      721 gaccatcaaa agttgtagat atagttgggt caatattaac tccagcttta cttatagctt
      781 tagcagtatt aatagttaaa ggtatcgtat ctccattagg agaaataaga gacagttctt
      841 taatacaaaa tgtatttgca gaaggtataa ctcaaggtta tcaaacaatg gatgcattgg
      901 cagctagtgt atttgcatca attataatca tgtcagtaat agctaaaggt tatacaggtg
      961 agaaagaaaa gatgaaagcc acaataagtg caggtgtaat tgcagtaata ggaatggcat
     1021 tagtttatgg tggattatgt tatcttggag caactgtgtc tcaacaatat ggacaagatg
     1081 ttcaacaaac agctttaata gttgcaataa cagcagcttt acttggaact acaggtaaaa
     1141 ttttattggc tattatagtt gcattagcat gtttaacaac agctataggt ctttcttcag
     1201 cagctggtca atatttctca acacttacag atggaaaatt aaagtatgag catatagtta
     1261 tagtagtttg tgtatttagt gcaatcatat caaactttgg agtaagtaca ataatacaat
     1321 tctcttctcc tatactaagt atggtatatc cagcaactat aactctagtt atactagcat
     1381 tatttagtaa taaaattaaa aatgacaacg tgttcaaatg tgctgcatac atggcattat
     1441 tagtaagtgt attaacagtt gcaacaagtt ttggtataaa aattcctcta gttaatagct
     1501 taccatttgc ttcattagga tttaactgga tagttccagt tgtaatagca ggaataatag
     1561 gaaactttat tccatctaaa agcagtaaaa ctgcataata ataaaaacta attgataatt
     1621 atggaattgc ctttaaatta tataggcaat tcctttttat atttaaaaga attattaaat
     1681 tattgtagat aaaagaacat tattaatcct atttgataaa tttaaaa
//
LOCUS       DAELLV010000378         1724 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.380, whole genome shotgun sequence.
ACCESSION   DAELLV010000378 DAELLV010000000
VERSION     DAELLV010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.380"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            170..1621
                     /locus_tag="KPD58_003640"
     CDS             170..1621
                     /locus_tag="KPD58_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HBG0267344.1"
                     /translation="MDFFNGILTSINNAVVYVNNYLWSYILIIMLVVIGIYFTIKTNF
                     VQFRYFIEMFRLLGDGTANKNAKKEGKISSFQAFCISTSSRVGTGNIAGIAIAVVAGG
                     PGAVFWMWLIALIGSASSFVESTLAQIYKVKDGQAFRGGPAYYMEQGLNKKWMGILFS
                     ILITICYGFVFNAVQANTVSLAFNNAFGISKMAMGIGLGILTAIVIFGGVHRVAKVSE
                     IIVPVFAGLYILVALAIVVMNITEIPSVIALIVESAFDFKGMAIGTFMGVVMTGVKRG
                     LFSNEAGMGSAPNAAATAHVTHPVKQGLIQSLGVFTDTIIICSCTAFMVLLYSGYSSS
                     GATGIELAQEALTHHIGPVGNIFIAVCIFLFAFSSIVGNYYYGESNMEFMSGSKTKLN
                     VFRVFVVGMVLFGSLTQVDVVWNLADLFMALMAIINLIAIALLGKYAFIALKDYTSQK
                     KSGIKDPVFIADEIEGLEKVSEWHRELSEEHLG"
BASE COUNT          596 a          169 c          333 g          626 t
ORIGIN      
        1 tttgaatata aaactgcaat atattaaaaa aattattcat tttaaacctt gtaatttgat
       61 tttataaata ttataattaa attaatgaat ttttaacaag gaattattca tttttttgat
      121 tttaagattt attgagaaaa caaaactaaa taggggggta attaatataa tggatttttt
      181 caatggtatt ttaacgagta ttaacaatgc agtagtatat gtaaacaatt acttatggtc
      241 gtatatctta attataatgt tagttgtaat tggtatttac tttactatta aaacaaattt
      301 tgtacaattc agatatttta tagaaatgtt tagacttctg ggtgatggta cagcgaataa
      361 aaatgcaaaa aaggaaggaa aaatctcttc atttcaagca ttttgtataa gtacatcttc
      421 aagagttgga actggaaata tagctggaat agcaattgca gtggtagcag gaggacctgg
      481 tgctgtattt tggatgtggc ttatagcatt aattggttct gcatcaagct ttgttgagag
      541 tacattagct cagatttaca aagtgaaaga tggtcaagct tttagaggtg gaccagcgta
      601 ttatatggaa caaggtctaa ataaaaaatg gatgggtatt ttattctcaa tacttataac
      661 tatatgttat ggttttgtat ttaatgcagt tcaagcaaat actgtttcac ttgcatttaa
      721 taatgcattt ggaataagta aaatggctat gggaataggt cttggaattt taactgcaat
      781 agttattttt ggtggagtgc atagagttgc aaaagtttct gaaataatag ttcctgtatt
      841 tgcaggatta tatatattag ttgctttggc tatagttgta atgaatataa cagagatacc
      901 aagtgttata gcattgatag ttgaaagtgc atttgatttt aaaggaatgg cgattggtac
      961 atttatggga gttgtaatga ctggagtgaa gagaggtcta ttttctaatg aagcaggtat
     1021 gggtagtgct cctaatgctg ctgcaacagc acatgtaact catcctgtaa aacaaggttt
     1081 aattcaatct ttaggtgttt ttacagatac tataataata tgtagttgta cagcattcat
     1141 ggtattatta tattcaggtt atagtagtag tggagcaact ggaattgaat tagctcaaga
     1201 agctttaact catcatatag gaccagttgg aaatatattt atagcagttt gtatattctt
     1261 gtttgcattt agttctatag ttggtaatta ctattatggt gaatcaaata tggagtttat
     1321 gagtggaagt aagactaaac ttaatgtatt tagagttttt gttgtaggta tggttttatt
     1381 tggttcactt actcaagtgg atgtagtttg gaatcttgca gacttattta tggcattgat
     1441 ggcgataata aacttaatag caatagcact tttaggaaaa tatgcattta ttgctttaaa
     1501 agattataca agtcaaaaaa aatcaggaat aaaagaccct gtgtttattg cagatgaaat
     1561 agaaggccta gaaaaggtat cagagtggca tagggaatta tctgaggaac atttaggata
     1621 aaaaatagta taaaattata aaaccatttt gaaagactag agtatgtata tactttatat
     1681 gaatataata ttgaattaat ttatattact ggaattttta attc
//
LOCUS       DAELLV010000379         1692 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.381, whole genome shotgun sequence.
ACCESSION   DAELLV010000379 DAELLV010000000
VERSION     DAELLV010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.381"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            317..1588
                     /gene="serS"
                     /locus_tag="KPD58_003641"
     CDS             317..1588
                     /gene="serS"
                     /locus_tag="KPD58_003641"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBG0267345.1"
                     /translation="MLDIKRIRENLDCIKKAMERRGEKDFNLDEVVKLDDERRKILQE
                     VEVMKNELNTASKNIPNLIKEGKDVENEKIKLKELSDKIKVIDQNLKEVEDKMEYLLM
                     RIPNVPHPEVPQGETDEDNVEVRTWGKTTTFDFESKAHWEIGTELGILDFETASKITG
                     SRFTLYKGLGARLERALLNFYLDTNTKVNGYTEVIPPFMANRNSFLGTGQLPKFEEDM
                     FKIEGLDYFMIPTSEVPLTNIHANEILKFEQLPINYTAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVEMVKIVAPEESYNELEKLTNNAETMLQLLNLPYRVVKICTGDLGFTASFK
                     YDVEVWMPSYNRYVEISSCSNCEDFQARRAGIRFKRDKDSKAEYVHTLNGSGLAIGRS
                     VAAILENYQQEDGSVVVPEVLRPYMGVSVIK"
BASE COUNT          719 a          170 c          300 g          503 t
ORIGIN      
        1 tataatataa aaaaatttat ataaaagtta ataactatat aagattgata aagaaaaagt
       61 caaaatagat aattgattag caaaatagat ataattataa tgaagttaaa tctagcaata
      121 ttctcctaaa attaaatttc atttatttaa ttaaatataa tttgacaatg ggcaaaaaag
      181 tgagtaaaat ataaataaaa taaaaataac aggttcggat aaagaaaaag gtttcttaac
      241 ctcaagcata aagtatatta tctaagttat ttgagattaa gagataaatc caatcttaag
      301 tactgaggag gaaaaaatgc ttgatataaa aagaataaga gaaaatttag attgtataaa
      361 aaaagcaatg gaaagaaggg gcgaaaaaga ctttaacctt gatgaagtag ttaagttaga
      421 tgatgaaaga agaaagattc ttcaagaagt tgaagttatg aaaaatgaat taaatacagc
      481 atctaaaaat ataccaaatc ttataaaaga aggtaaagat gtagaaaatg aaaagattaa
      541 actaaaagag ttatctgata aaattaaggt aatagaccaa aatcttaaag aagtagaaga
      601 taagatggaa tacctattaa tgagaatacc aaatgtacca catccagaag taccacaagg
      661 tgaaactgat gaagataatg ttgaagtaag aacttgggga aagactacta cctttgattt
      721 tgaatctaag gctcattggg aaattggaac agaattaggt atacttgatt ttgaaacagc
      781 tagtaaaata actggttcaa gatttactct ttacaaaggt ttaggtgcta gattagagag
      841 agcattatta aatttttact tagacacgaa tacaaaagta aatggatata cagaagttat
      901 tccaccattt atggcaaata gaaatagttt tttaggtaca ggacagttac ctaagtttga
      961 agaagatatg tttaaaatag aaggtctgga ctattttatg attccaacat cagaagttcc
     1021 tttaactaat atacatgcta atgaaatact taaatttgaa caattaccga taaattatac
     1081 agcttatact ccatgtttta ggtcagaagc tggttctgct ggaagagata caagaggttt
     1141 agtaagacag catcagttta acaaagtaga aatggttaaa atagttgcac cagaagaatc
     1201 ttacaatgag ttagaaaagc ttactaacaa tgcggaaact atgctacaat tattgaatct
     1261 gccttatagg gtagtaaaga tatgtacagg tgatttagga tttacggctt cttttaaata
     1321 tgatgtagaa gtttggatgc caagttacaa taggtatgtt gagatttcat cttgttcaaa
     1381 ctgcgaagat ttccaagcta gaagagcagg aataagattt aaaagagata aagattctaa
     1441 agctgaatat gtacacacat taaatggctc tgggttagct attggaagaa gtgttgcagc
     1501 tatattagaa aattatcaac aagaagatgg ttctgtggtt gttcctgaag ttttaagacc
     1561 atatatggga gtttctgtta ttaaataaga gttaaattat gtatttttaa gcaatacaat
     1621 aatatagagg taataaaaaa taattaatat aaaagtgata cttctaatat agaagtaata
     1681 aaaaaatagt ta
//
LOCUS       DAELLV010000380         1654 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.382, whole genome shotgun sequence.
ACCESSION   DAELLV010000380 DAELLV010000000
VERSION     DAELLV010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.382"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            7..636
                     /locus_tag="KPD58_003642"
     CDS             7..636
                     /locus_tag="KPD58_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HBG0267346.1"
                     /translation="MDEKITKNNYNVPFDYVRKKLKELDPHTISNLSNIPFNNDLNRF
                     TLNFFDRQYIIDYPSGEVWNSNGSLCNNFELKILIVRYLINAKGIPRTNKYVTFKEIP
                     GGNVYYPNFLNRTLYRLSEVFTSQIDKYKLVMEKIGAEKVNMGDLAYRFTYMGNTSMV
                     FILWFGDDEFPPNSNILFDSNIVYYFNAEDLAVVPDTAINAILNKINQL"
     gene            704..1537
                     /locus_tag="KPD58_003643"
     CDS             704..1537
                     /locus_tag="KPD58_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HBG0267347.1"
                     /translation="MNVENQSLSDIEIILSHRYDNGADYWTTPDKRLIKGSPFSTLDS
                     VLYLLELGVNPDDTLLKECADLIFSTWREDGRFKLYPNGGIYPCHTANAANVLCHMGY
                     ASDIKMQKTFQHFLDTQYTDGGWRCNKFSFGRGPETEYSNPLPTLNILNAFRFSNYLN
                     KESKLDKAVDFLLDHWTIKKPIGPCHYGIGTLFMQVEYPFRNYNLFLYVYVLSFYNRA
                     KKDNRFLEALKILESKMIGNKIVVERVVPKLSKLSFCKKGESSEIATTHYYEILKNLE
                     K"
BASE COUNT          634 a          217 c          239 g          564 t
ORIGIN      
        1 aaaattatgg atgaaaagat aacaaaaaat aattataatg ttccatttga ctatgttcga
       61 aaaaaactca aagaattaga ccctcataca attagtaatt taagtaatat acctttcaat
      121 aatgatttaa atagattcac actcaatttt tttgatagac aatatattat agactatcca
      181 agtggagaag tttggaatag taatggtagc ctttgcaata actttgagct taaaatcttg
      241 atagttagat atttaatcaa tgctaaaggt atccctagaa ctaataaata tgtaactttt
      301 aaagaaatac caggaggcaa tgtttattat ccaaattttt taaacaggac tctatatagg
      361 ctttcagaag tttttacatc tcaaatagac aaatataagt tagttatgga gaaaattgga
      421 gctgaaaaag taaacatggg agatttagct tataggttta catatatggg taatacatct
      481 atggtattta tactttggtt tggagatgat gaatttcctc caaattcaaa tatattattt
      541 gattctaata tagtttatta cttcaatgca gaagatttag cagttgttcc tgatacagct
      601 ataaatgcaa ttttaaataa aataaatcaa ttataaatat aaataatatt ataataaagt
      661 ttttgataga tttaatagaa tataatatga aatggagtaa aaaatgaatg tagaaaatca
      721 atctttatca gatattgaaa taatattatc acacaggtat gataatggtg cagactattg
      781 gactacacct gataagcgtt taataaaagg ttctcctttc tctactcttg atagtgtact
      841 ttatctatta gaattaggtg tgaaccctga tgatacttta cttaaagaat gtgctgacct
      901 gatatttagc acatggcgag aagatggacg tttcaaacta tatccaaatg gaggtattta
      961 cccttgtcat actgcaaatg ctgctaacgt actctgtcat atgggatatg catctgacat
     1021 taaaatgcaa aaaacgtttc aacatttttt agatacacag tatactgatg gtgggtggcg
     1081 ttgcaataaa tttagttttg gtcgaggtcc agaaactgaa tactcaaatc cactaccaac
     1141 tcttaatata ttaaatgcat tccgttttag taattatcta aataaagaat caaaacttga
     1201 caaagctgta gattttctac ttgaccattg gactattaaa aaaccaatcg gcccttgtca
     1261 ctatggaatt ggcacattat ttatgcaagt tgaatatcct ttccgtaatt ataacctatt
     1321 cctttatgtc tacgttttat cattttataa tcgtgcaaaa aaggataata gatttttaga
     1381 agcattgaag atacttgaat ctaaaatgat aggtaataaa attgtcgttg aacgtgtagt
     1441 tccaaaacta tctaaattat ctttttgtaa aaaaggcgag tcaagtgaaa tagccacaac
     1501 acactattac gaaatattaa aaaacctaga aaaataatag ttatatagat aaaacatatt
     1561 aaaaaatagt ggctttaaat tttttaaagt cactattcta tataaatata acaatataaa
     1621 aaatatatta aactatttac tcttttttaa aacc
//
LOCUS       DAELLV010000381         1651 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.383, whole genome shotgun sequence.
ACCESSION   DAELLV010000381 DAELLV010000000
VERSION     DAELLV010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.383"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            178..837
                     /gene="cprR"
                     /locus_tag="KPD58_003644"
     CDS             178..837
                     /gene="cprR"
                     /locus_tag="KPD58_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CprR"
                     /protein_id="HBG0267348.1"
                     /translation="MSKILVVDDELDMLKLIENVLKRDGHEVKVISDVEKAMNMDFNY
                     FNLIILDVMMPKMDGFEVCKTIRNKVDCPILFLTAKNMESDIMYGLGIGADDYITKPF
                     GVGELRARITAHLRRESREKKNLLTISNVQFNFLGKEVSIDGKKINFTKSEYLICEFL
                     AKNKGQVFSKERIYESVYGFDGESDISTITEHIKNIRSKLKQYDIYTIETVWGIGYKW
                     V"
     gene            1048..>1651
                     /locus_tag="KPD58_003645"
     CDS             1048..>1651
                     /locus_tag="KPD58_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBG0267349.1"
                     /translation="MDRLKNIRKSLLQYTFLFLLIGITIYLVFKTLDIKMLSNVMVMV
                     DKKFLFIGALAIMCHIMLEGVVMKIIIESTHKVNIRFIGFKLAIMGFYYNLITPFASG
                     SQPVQIYVLKKCKMPLSKASAVVTNKSIIFQIVVTFYCTILVLMNFAMLEKQMKMIMP
                     LVFLGIAINSFVLIMIILVILNPKKIKYFMRITIKHLSKIK"
BASE COUNT          649 a          124 c          282 g          596 t
ORIGIN      
        1 aaatgaatat aaagtttttg aaatataaag tttttgtatc taaaatattt tatttgtaaa
       61 atagtaatta attattgtaa gctataatat gagagtatta tggttaatgt gaattttaaa
      121 gatattatta aatatataaa ttagaatgct attagatgga taaatggagg agttattatg
      181 tctaagatat tagtagtaga tgatgagtta gatatgttaa agttgattga aaatgtgttg
      241 aaaagagatg gacatgaagt aaaggtaatt agtgatgtag aaaaggctat gaacatggat
      301 tttaattatt ttaatctaat aatattagat gttatgatgc ctaagatgga tggttttgaa
      361 gtatgtaaaa ctataagaaa taaggttgat tgtccaatac tgtttttgac agcaaaaaat
      421 atggaaagtg atattatgta cggtcttggt ataggtgcag atgactatat aaccaaacct
      481 tttggtgtag gagaattaag ggctaggata actgctcatt taaggcgaga aagtagagaa
      541 aaaaagaatt tattaactat ctcaaatgtt caatttaact ttttaggcaa agaagtttca
      601 attgatggta aaaaaattaa ttttactaaa agtgaatatc taatatgtga atttctagca
      661 aaaaataagg gacaagtatt ttcaaaagaa agaatatatg agtcggtata tggttttgat
      721 ggagaaagtg atataagtac tatcacagag catataaaaa atatacgaag caaattaaaa
      781 caatatgata tttatacaat agaaactgta tgggggatag ggtataaatg ggtgtaataa
      841 gtataagtgt tgtttagtga aaatattata tatatctaca aaatgtttgt aaaatattaa
      901 tctatatatt aaaatttaat ttatagatta atattttttg taaatgacaa aattgtaagg
      961 tattttgtta taaaatatta agattcatag tgtatactaa taatatagta tttagtataa
     1021 atcttgagca aaggatggtt atttctaatg gatagactaa agaatattag aaagagttta
     1081 ctacagtata catttttatt tttgttaata ggtataacaa tttacctagt gtttaaaaca
     1141 ttggatatta aaatgctatc aaatgttatg gttatggtag ataagaagtt tttattcata
     1201 ggtgctttgg caataatgtg tcacataatg cttgaaggag tagtaatgaa gataattata
     1261 gaaagcactc acaaagttaa tattagattt attggtttta agctagccat tatgggattt
     1321 tattataatt taataactcc ttttgcttca ggaagccaac cagtacagat atatgtactt
     1381 aaaaaatgta aaatgccact tagtaaggca agtgcagttg ttaccaataa gtctattatc
     1441 ttccaaatag tagttacttt ttattgtaca atacttgttt taatgaattt tgctatgtta
     1501 gaaaaacaaa tgaaaatgat tatgcctcta gtgtttctag ggatagccat taattcattt
     1561 gtcttaatta tgataatatt agttatttta aatcctaaaa aaataaaata ttttatgaga
     1621 attactataa agcatttatc aaaaattaag t
//
LOCUS       DAELLV010000382         1651 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.384, whole genome shotgun sequence.
ACCESSION   DAELLV010000382 DAELLV010000000
VERSION     DAELLV010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.384"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            4..483
                     /locus_tag="KPD58_003646"
     CDS             4..483
                     /locus_tag="KPD58_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBG0267350.1"
                     /translation="MSNKLIKNLLSGILQILFFLLGLVIVVGGFKSFMYLCFSGEATL
                     QGTIYGILMFILGVSYFIIIKSLIEVLGSSEYSLFVKENVKRFRIIGYLLLLNSLIEF
                     ISTFGTTGKGMRFLDLGFGFYFTVPVFVYFITSLMSFVIADGFVKAIKIKEDNDLTI"
     gene            1121..1498
                     /locus_tag="KPD58_003647"
     CDS             1121..1498
                     /locus_tag="KPD58_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0267351.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDVTVTSKDVIEAMEQKYGWKQTTT
                     LTLLSRLVKRGFLDAQKIDRYTHYTIRVKHKDYLSFETKDFLSNIHDDSLRSLISALH
                     DDENIGKDKLDFLESYFDNLKDE"
BASE COUNT          661 a          130 c          242 g          618 t
ORIGIN      
        1 atgatgtcta ataaacttat aaaaaattta ctaagtggta tattacaaat tttatttttt
       61 cttttaggat tagtaattgt tgtaggagga tttaaaagtt ttatgtacct ctgttttagt
      121 ggagaagcaa ctctacaagg tactatttat ggaatattaa tgtttatttt aggtgtatca
      181 tattttatca ttataaaatc attgatagaa gtattaggta gttctgaata tagcctattt
      241 gtaaaagaga atgtaaaaag atttaggatt attggatatc ttttgctttt aaactctcta
      301 attgagttta tttcaacttt tggtactact ggaaaaggaa tgagatttct agatttagga
      361 tttggatttt attttactgt tccagtattt gtatatttta taacttctct gatgagtttt
      421 gtaattgcag atggatttgt aaaggcaata aagataaaag aggataatga tttaactatt
      481 taggtgtgac actttaatat ataggagata aataacccga aaagagtgac taactagtaa
      541 tttaaaataa aatatattag ggtataatac ataaactata atgctaaaat taaaagatta
      601 taatactaaa atataattat gttttaatta aaataaagaa ctttattagt agatattaaa
      661 agcacatttt tttataaaag tactttttta taatttatga aaggtataat tataaaagtc
      721 ttattcaata aaatttataa ttaatttaga tatatttgta aataatcagt ataaaataga
      781 tgttttaata tcataaaggc ataattagaa ggttattaaa atttattaga tattactcta
      841 gtttatttta aaaaaaagta ttataatatt ccttgtgtga aaatatagga atttttatta
      901 aatagtttta atgtgtaaat aacattctac actattgtaa ttaaatatgt aaaaagtaaa
      961 atatttaaat agatatattt taaaatctaa cttttaatat tgtaaaaaaa ttagaaattt
     1021 aacaaaaagt acaaaaattc acattttttt cataaagtgt ttgactaaaa aattcacatg
     1081 ttgtagaatt aaggtagcag gagttttgag ggaggtgtaa ataatgataa gaaaaatacc
     1141 acaagcagaa ctaaaagtaa tgaaattcat ttggaaagta gatgttacag tgacatcaaa
     1201 agatgttatt gaagctatgg aacaaaaata tggctggaaa caaaccacca cactgacact
     1261 tttatctagg ctggtaaaaa gaggattctt agatgctcaa aaaatagaca gatatacaca
     1321 ttacacaata agagtaaaac acaaagatta tttaagtttt gagacaaagg atttcctaag
     1381 taatatacat gatgactctt taagaagctt aatttcagca ttacatgatg atgaaaatat
     1441 aggtaaagat aagctagatt ttctagaaag ttattttgat aatttaaagg atgaataatt
     1501 atatgctata tgtttttgaa aatcttataa agacaactat tgtatgtagt cttggtattt
     1561 gtctattatt atttttgaaa agatatttat ttaagaagtt tagtaagaga ttcaattatt
     1621 atatttggtt gataatagtc tttagaatgt t
//
LOCUS       DAELLV010000383         1599 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.385, whole genome shotgun sequence.
ACCESSION   DAELLV010000383 DAELLV010000000
VERSION     DAELLV010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.385"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            393..1598
                     /locus_tag="KPD58_003648"
     CDS             393..1598
                     /locus_tag="KPD58_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBG0267352.1"
                     /translation="MSKKVLIMSASTGGGHNRAALAIKEELTSKTLDGEPIECEIIDS
                     LKLVNNTMDKIISRGYEKSAIYTPKAYGSVYRLSETNLLSKNEFKDNLLITFMAKKFK
                     KLIRSEKPDLIIGTHPFPMIALSTLKKNFNLHNNESNAYTEHFYKHYTNTINVPPLIS
                     VLTDYTTHSTWIQNEIDYYIVGHEYVKELLVFDGVEPSKIRTFGIPVEKSFLSHRDKG
                     IVLSELNLSPDKLTVLLMGGSFGAGNIKETLDELLDTDRDFQILVITGKNESLKEKIE
                     KKLMSRYHDKNVCVLGYTDKMNDILASIDVLVSKPGGLTTTEALLKDVPMIVPYYIPG
                     QEEENLDFLSNCGAALRTTKKYNLSILLKVLIDDPSRLEMLKRNIKSIRKSNSAQNIA
                     NLILNIISG"
BASE COUNT          598 a          204 c          214 g          583 t
ORIGIN      
        1 ttcctttttt caaggacaag taatactata tcatctttat ctattctagt caatataatt
       61 ttttactttt tttatttttt ttatttatca tgtattcttt atatttaatt tttaaaactt
      121 aatgctaagt aaacctgttt atttcataaa actaatatat attctatgct ctattttcct
      181 ttttattcta tatttaaatt ttttatttac aagaatattt ttaatataac atatttgata
      241 aaattaattt acatttcttt cacttgtaat ttttgcaatc ttgtattaga ttaatattaa
      301 aaataattta tcaataacct tattcacaaa gacagttttt atatatcaaa atataatata
      361 aatactatgc tacattttag gaggatttta ttatgagcaa aaaagtatta ataatgtctg
      421 cttccactgg tggtgggcat aatagggctg ctctagctat taaagaagaa cttacttcca
      481 aaacacttga tggagagcca attgaatgtg aaataataga tagtttgaaa ttagttaata
      541 atacaatgga taaaataatt tcaagaggat acgaaaaatc tgctatatat actcccaaag
      601 cctatggaag tgtatataga ttatctgaaa caaatctttt atcaaaaaat gaatttaaag
      661 ataatctact aataacattc atggctaaaa aatttaaaaa acttattaga agcgaaaaac
      721 cagacttgat tataggaacg catccttttc caatgattgc tctaagtaca ttgaaaaaaa
      781 actttaattt acataataat gaatcaaatg cttacacaga gcatttttat aaacattata
      841 caaataccat taatgttcct ccgcttattt ctgtattaac cgattatact actcattcta
      901 catggataca aaatgaaatt gattactata ttgttggtca tgaatatgta aaagaattat
      961 tggtgtttga tggagttgaa ccgagtaaaa ttagaacatt tggaatccct gttgaaaaat
     1021 cttttctctc tcacagagat aaaggtattg ttctttctga gttgaattta tctcctgata
     1081 aattaactgt tttacttatg gggggtagct ttggggctgg aaatataaaa gagacacttg
     1141 atgaattact ggatactgat agagactttc aaatacttgt aattactggt aagaatgaaa
     1201 gcctaaaaga aaaaatcgaa aaaaaactta tgtctcgtta tcatgataaa aatgtatgtg
     1261 ttcttggata cactgataag atgaatgata ttttagcttc tatagatgta ttagtaagca
     1321 aacctggtgg acttactaca actgaggctc ttttaaaaga tgtacctatg atagttcctt
     1381 attatatacc aggtcaggaa gaagagaatc ttgatttctt atctaattgt ggtgcagcac
     1441 ttagaactac aaaaaaatat aacttgtcaa tattactaaa agttttaata gatgacccta
     1501 gcagattaga aatgctaaaa agaaatatta aatcaataag aaagtcaaat tcagcccaaa
     1561 atatagctaa tttaatttta aatataattt ctggttgat
//
LOCUS       DAELLV010000384         1597 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.386, whole genome shotgun sequence.
ACCESSION   DAELLV010000384 DAELLV010000000
VERSION     DAELLV010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.386"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            164..1504
                     /locus_tag="KPD58_003649"
     CDS             164..1504
                     /locus_tag="KPD58_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG0267353.1"
                     /translation="MIKSSLFWNIFIAISFMIEWFLCKKMLDYTSEKRISNKKINFCM
                     VGIIVFADIIYFFDVPPNIRVIISMIITIAFYKISYDVGFIKSVLISLIYWMLLIATD
                     ALSISLTVWLNSINDMSSMGTGDIYRIESIILGKILLIVSLLLYRALKIKLNIEINKR
                     TLFYIVIPIVANIASFFVIFEYIFNPGSGSLIHNFQFVMISVVLLISSITLVLIAKKL
                     QQYNTLVVENNMIKNTLKYENEYYEDIEKKYLKTRLLSHDMKNHILCINAMIKKGIDV
                     TSYLNNLQVEIQSNDLLFNTGNFILDAILSEKKEVCDKNDIELKIGINFSCCEAVELV
                     DICHIFSNILDNAIEACNKIDSGNRVICVKGDVMQNFYLIRVENTKMNKIITKNGDIL
                     TDKKGSLHGLGIKSVKNSVNKYGGNVVIEYDDKKFVVKIAIPLRNVKKAVNHVI"
BASE COUNT          623 a          122 c          259 g          593 t
ORIGIN      
        1 attcaattta gcaaatttag tgatataatt aaaaaagaaa agaaatatgg acttatttgg
       61 aataattttg acaatctatt tactacacgt gtagagtcgt atataattaa attatacata
      121 aaatataaaa aaataattta gtaatacgtt aggagaaagt attatgataa agtcaagctt
      181 attttggaat attttcattg cgatatcctt tatgatagaa tggtttcttt gtaagaaaat
      241 gctagattat acaagtgaga aaaggatttc taacaaaaaa ataaactttt gtatggtagg
      301 tattattgtt tttgctgaca taatatattt ttttgatgta cctccaaata taagagtaat
      361 tatatctatg ataattacta ttgcatttta taaaatctca tatgatgtgg ggtttataaa
      421 gtctgtatta atatccttaa tttattggat gttactaatc gccacagatg ctttgagtat
      481 atctttaaca gtttggctta attctataaa tgacatgagt agtatgggga cgggagatat
      541 atacagaatt gagtctataa tattgggaaa gatacttttg attgtgtctt tgcttttata
      601 tagagcctta aagataaaac ttaatataga aataaataaa aggacattat tttatatagt
      661 tattcctata gtcgctaata tagcatcttt ttttgtaatt tttgaataca tatttaatcc
      721 agggtctggt agcttaatac ataatttcca attcgttatg atttctgttg tattgcttat
      781 atcaagtatc actttagttt taattgctaa aaaattacag caatataata ctttagttgt
      841 agaaaacaat atgattaaaa atactttgaa gtatgaaaat gagtattatg aagatataga
      901 aaagaaatat ttaaaaacta gattactaag tcatgatatg aaaaatcaca tattgtgtat
      961 aaatgctatg attaaaaaag gtattgatgt tacttcttat ttaaataatt tgcaggtaga
     1021 aattcagagt aatgacttgt tatttaatac tggtaatttt atattagatg ctatattaag
     1081 tgaaaagaaa gaggtttgtg ataagaatga tatagaacta aaaataggaa taaattttag
     1141 ttgttgtgaa gctgttgaat tagtggatat atgtcatata ttttcaaata ttttagacaa
     1201 tgcaattgag gcatgtaata aaattgatag tggaaataga gttatttgtg ttaaaggtga
     1261 tgttatgcaa aatttttatt taataagagt tgaaaatact aagatgaata agattattac
     1321 taaaaatgga gatattttga cagacaaaaa aggttctctt catggacttg gaataaaaag
     1381 tgtaaaaaac tctgtaaata agtatggtgg taatgttgta attgaatatg atgataaaaa
     1441 atttgtagtt aaaattgcta ttccacttag aaatgtgaag aaagcagtta atcatgtgat
     1501 ttaaatgtat ataaatttga tgtgtagaaa tttaatatat aagaattaat gtataaaagt
     1561 gatgcataga agtttaatat ataagaatta atgtata
//
LOCUS       DAELLV010000385         1590 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.387, whole genome shotgun sequence.
ACCESSION   DAELLV010000385 DAELLV010000000
VERSION     DAELLV010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.387"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1126
                     /locus_tag="KPD58_003650"
     CDS             <1..1126
                     /locus_tag="KPD58_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBG0267354.1"
                     /translation="TIEEYKNKYFTGNKIVLSLNSFDNKELYYHETENILNLINEIDK
                     LTSSKIFKTIEDKNDLALESYYSSVIEGAFSTRKIAKSIIRGKMKPSNKSEYMIYNNH
                     RALEYGLDNLDKLYSHKFIYDLHHILGENCLDSEEYEYRTEKVYVCDSKGEIIHIGLE
                     PLKIYDFMSKLIDFMENSKVSNLIKSAIIHFYFVYVHPFSDGNGRTSRALSYLYLIDK
                     GYDTFKEFSISYMISKNRTKYYKAILDVENKGNNLTVFIEFMLKSIIQSINEMRNMHD
                     RKSLESILKEELFENDITLSATEEHILKYICNKDNYSMTLENYIKKNKSRYLKAGIKE
                     IELVDQLMEVFSNLEEIEILSKEKNIYKVNDKYLKMLDID"
     gene            1152..1340
                     /locus_tag="KPD58_003651"
     CDS             1152..1340
                     /locus_tag="KPD58_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0267355.1"
                     /translation="MNNKKKAFLISGAINVISLIIVFSLFHYNIIENSTQNMIITVIL
                     IFIFEMIKIKIIEKYYKL"
BASE COUNT          674 a          132 c          231 g          553 t
ORIGIN      
        1 gacaatagaa gaatacaaaa acaaatactt tactggaaat aaaatcgtac tttctctaaa
       61 tagttttgat aataaagaac tatactatca tgaaactgaa aatatattaa atttaataaa
      121 tgaaatagat aaattaacat caagtaagat atttaaaaca atagaagata aaaatgattt
      181 ggcactagag tcttattatt ctagtgtaat agaaggtgct ttttcaacta gaaagattgc
      241 aaaatctata attagaggaa agatgaaacc tagtaataaa agtgaatata tgatttacaa
      301 taatcataga gccttagaat atggattaga caacttagat aaattatata gtcacaaatt
      361 tatatatgat ttgcatcata tattgggcga aaactgcttg gattcagaag aatatgagta
      421 tagaactgaa aaagtatatg tatgtgattc aaaaggtgag attatacata tagggcttga
      481 acctttgaag atatatgact ttatgagtaa attaatagat tttatggaaa atagcaaagt
      541 aagtaattta attaaaagtg ctataattca tttttacttt gtatatgttc atccttttag
      601 tgatggtaat ggaagaacat caagagcctt atcatatcta tatttgattg ataaaggata
      661 tgatactttc aaggagtttt caatatcata tatgatatct aaaaacagaa ctaagtatta
      721 taaggctata cttgatgtag aaaacaaagg aaataattta actgtattta tagagtttat
      781 gctcaagtct ataatacaat ctattaatga gatgcgtaat atgcatgata gaaaatcttt
      841 agaaagtata ttaaaagaag aattgtttga aaatgacata actctatcag caacagaaga
      901 acatatatta aaatatattt gcaataaaga taactattct atgacattag aaaattatat
      961 aaaaaagaat aaaagtagat acttaaaggc tggaataaag gaaattgaat tagttgacca
     1021 gttgatggaa gtgtttagta atcttgaaga gatagagatt ttgtctaaag aaaaaaatat
     1081 atataaagtt aatgataagt acttaaaaat gcttgatata gactaattta gataatctgg
     1141 agagggaata tatgaataat aagaaaaagg catttttgat ttctggggct ataaacgtaa
     1201 tttcattaat tattgttttt tcattgtttc attataatat tatagaaaat agtacacaaa
     1261 atatgataat aactgtaatc ttaattttta tttttgaaat gataaaaata aaaatcatag
     1321 agaaatacta taaactataa acaagaaata tcttttctaa gagcatattg tacaatatgc
     1381 tcttaattta gttatataga tttaatataa ataatgtatt ttttgtagat gatttatgta
     1441 agatttatga ccaatttgtg tatgatttat gttctttaat catgacaata ttatattgta
     1501 aaaaataaat ttaataaaaa atgacttgga atttatattc taggtctttt ttatataaaa
     1561 atttaataag tttcaaatgt tggtaacttt
//
LOCUS       DAELLV010000386         1584 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.388, whole genome shotgun sequence.
ACCESSION   DAELLV010000386 DAELLV010000000
VERSION     DAELLV010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.388"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            163..1521
                     /locus_tag="KPD58_003652"
     CDS             163..1521
                     /locus_tag="KPD58_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HBG0267356.1"
                     /translation="MKAKSTSKFELDGIPPLKEAIPLGLQHLMAMVIGSCLPAILIAN
                     AAGLNHKMSTLLIQAALVFAGLSTLLQLYPIPLFKGFKLGGGIPVIMGATAMFIGAGV
                     SVASKYGLPVLFGSQIIAGIVIIIIGFGLKKIRFIFTPAVSGTIVACMGLGLFPIAIK
                     NLAGGMGNETFGNPINFIVGISVSLMILGLNKYGKGLFKDASILVSIIFGYILSLILG
                     IVNFSSIQEFTLVALPKPLAFGLDIRLEVVVMFSIIYLVEIADIMGACTLSAVGGLNR
                     QVTDEELSSAVLGNGVLSTIGALFSATPMGMFGQNSAIVSNTKVVSKFVLAIGGIGLF
                     LAGISPLLANLIRTIPPCVVGGATLVIFSTLTTSGLRLVSMDGFNQENSMILGLSMAS
                     GIGFMVAPQVLEKFPKFIETLLADSSVVSGAMVAIIIQALYMVKFPGNKSDKVEDKNK
                     SL"
BASE COUNT          529 a          186 c          294 g          575 t
ORIGIN      
        1 tgtatttgtt aataattaaa tttattgcat ctgtttttta agactaaaaa cttaaatata
       61 tgttggagta tagttgatat ttatgaacat cattaattta atatattaga tttttaagta
      121 tttatataat tcaataagtt ttatgtaacg ggagttttta ttatgaaagc aaaaagtaca
      181 tcaaaatttg aattagacgg aataccacca ctaaaggaag caataccttt agggttacaa
      241 cacttaatgg caatggtaat aggtagttgt ttaccagcga tattgattgc aaatgcagct
      301 ggacttaatc ataagatgtc aacattattg attcaagccg cattagtttt tgcaggatta
      361 tcaactttat tacaattata tccaatacct ttgtttaaag gttttaaatt aggaggagga
      421 ataccagtta taatgggagc tacagctatg tttataggag ctggggtatc tgttgcaagt
      481 aagtatggac taccagtatt atttggctct caaataatag ctggtatagt aataattata
      541 attggttttg gtcttaaaaa aatacgattt atatttactc cagctgtatc aggaacaata
      601 gttgcatgta tgggtcttgg actttttcca atagctataa aaaacttagc tgggggtatg
      661 ggcaatgaga cttttgggaa tccaattaat tttatagttg gaatatctgt atctcttatg
      721 atattaggat taaataagta tggaaaagga ttatttaaag atgcatcaat attagttagc
      781 ataatttttg gatacatttt atcattaatt ttgggtatag taaatttttc ttcaattcag
      841 gagtttactt tggttgcact tccaaaacca cttgcttttg ggctagatat aagacttgaa
      901 gttgttgtaa tgttttcaat aatatattta gttgagatag ctgatataat gggtgcttgt
      961 acactttctg cggtaggagg gcttaataga caagtaacag atgaagaact ttcttctgct
     1021 gttttaggaa atggagtact ctctacaata ggtgcattat tttctgcaac tccaatgggg
     1081 atgtttggac aaaattctgc aatcgtttcc aatacaaagg ttgtaagtaa atttgtacta
     1141 gctattggag gaattggatt atttttggca ggaatatcgc ctttattagc taatttaata
     1201 agaacaattc ctccctgtgt ggttggagga gcaacactcg ttatattttc aactcttaca
     1261 acatctggtt taagacttgt tagtatggat ggatttaatc aagaaaatag catgatatta
     1321 ggtctttcta tggcttctgg tattggattc atggtagctc ctcaagttct tgaaaaattt
     1381 ccaaaattta tagagacatt acttgcagat tcaagtgtag tttcaggagc aatggtagcc
     1441 ataataattc aagctttata tatggtaaaa tttcctggaa ataaaagtga taaggttgag
     1501 gataaaaata aaagtttata atgtaaaggt ttaatatatg tgaattaaat atactacaat
     1561 tataaatata aataaaagat ttat
//
LOCUS       DAELLV010000387         1574 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.389, whole genome shotgun sequence.
ACCESSION   DAELLV010000387 DAELLV010000000
VERSION     DAELLV010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.389"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(175..390)
                     /locus_tag="KPD58_003653"
     CDS             complement(175..390)
                     /locus_tag="KPD58_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0267357.1"
                     /translation="MHINQTGFISNELGIKQQQKKEPTSNSFSYNAPSSWKPVGRDSV
                     QYNCGFELIATLVRGGSSWAFNFIVQP"
     gene            complement(573..917)
                     /locus_tag="KPD58_003654"
     CDS             complement(573..917)
                     /locus_tag="KPD58_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0267358.1"
                     /translation="MNYRLKKSVTTILSLLIMTILLPTNASASKVEDSSMKVMYSEKQ
                     VTDMDKLYDMAKNGITDVKSSNEKGIIKNARTGESIYVDSISTTQLLEVKQSKNLTSK
                     IYSKTVFLILIQ"
     gene            1044..1427
                     /locus_tag="KPD58_003655"
     CDS             1044..1427
                     /locus_tag="KPD58_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0267359.1"
                     /translation="MRKKFFIGILIVLIISVIAILNIKNDKNVFIGESENWKAKFVTT
                     EGHSKLEIKYKNSKDVVEQKFNYSYQFIDGEDAVVVVKNEILEAGQEKAIKIQRPGLY
                     SNRESSYKLIISWNGNEETINLKKK"
BASE COUNT          579 a          204 c          198 g          593 t
ORIGIN      
        1 atacattttt tataatttat taaaataaaa ttcatatatt aagaaatatt aatagtaaat
       61 aagcaattgt tactataaca taggaattat ctttaaaaac acaaattaat ttacattctc
      121 taattttaaa gtatatgtat atctttgatt ttaataaaat tcacttaaaa tattttaagg
      181 ttgaacaatg aaattgaatg cccaagaact tcctcctctt actaatgttg caattaattc
      241 aaacccacaa ttgtattgaa ctgaatctct acctactggt ttccaactag aaggtgcatt
      301 atatgagaaa gaatttgatg ttggctcttt cttttgttgc tgcttaattc ctagctcatt
      361 agaaataaat ccagtttgat ttatatgcac ccttctattt gttacacgaa tctggccgtc
      421 tgcaatagac cagtttccag aaactttact aacggaaata tattgaaatg ctccatctcc
      481 ttttgatatt gtatatgtaa ctgtaccaga tgcttttaca ccaccagttt tatcccatcc
      541 atcttctgat atacttgctt ttctaagagg ctttattgta ttagtatcaa aaaaactgtc
      601 ttagaatata tctttgatgt taaattttta gattgtttaa cttctaataa ttgagttgtt
      661 gatatagaat caacgtatat ggactctcct gttcttgcat ttttgattat acctttttca
      721 ttggaacttt taacatcagt aattccattt ttagccatat cgtataattt gtccatgtct
      781 gttacttgtt tctctgaata cattactttc atagatgaat cttctacctt actagcactt
      841 gcatttgttg gtaatagtat tgtcattatg agtaaagata aaatagttgt tacagatttt
      901 tttaacctat aattcatatt aatccttact ttttatttgt tataacagac attctattgt
      961 aatagaatat aaatccacaa tatttttaaa attagtattg aataactatt ctcatgttat
     1021 aattattcaa aggagggatt gatatgagga aaaaattttt tataggtatt cttatagttt
     1081 taataatctc tgtgatagct atacttaata taaaaaatga taaaaatgtc tttattggag
     1141 aatcagaaaa ttggaaagct aaatttgtaa ctactgaagg acactcaaaa ttagaaataa
     1201 aatataaaaa tagtaaagat gtagttgaac aaaaatttaa ttattcctat caatttatag
     1261 atggtgaaga tgcagttgta gttgtaaaaa atgaaatttt agaagcaggt caagaaaaag
     1321 caataaaaat acaaagacca ggtctttatt caaatagaga atctagttac aagctaatta
     1381 tatcttggaa tggaaatgag gaaactataa acttgaaaaa gaaataacca tattatcaat
     1441 ttttaaaaaa ttattattca aattcttata aaaagagtgt ttagaaagat gaaacactct
     1501 tttttgtata agtttttgaa aactataaat gaaatatatc aaaatcgttc gaaaaattac
     1561 tgaaataaac tctt
//
LOCUS       DAELLV010000388         1566 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.390, whole genome shotgun sequence.
ACCESSION   DAELLV010000388 DAELLV010000000
VERSION     DAELLV010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.390"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            49..1530
                     /gene="cls"
                     /locus_tag="KPD58_003656"
     CDS             49..1530
                     /gene="cls"
                     /locus_tag="KPD58_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBG0267360.1"
                     /translation="MFDISFFEMSIYEIAVTTIYIINFMVILNLIFREKRNINTTLTW
                     LLILVLIPALGFILYIAFGRNISKNNMFRLKEKDDKIIKSNILDTQVKLQSTSEIDSD
                     IHQHKDMIYALANSNNAHYTNNNDVWIYAESSQFFNSLLEELKKAKKYINIQFYIFKD
                     DKIGTEIIDILIDKAKEGVEVRLLFDAVGGRTLKNSTLSRLKESGVKVGSFFPSFLKI
                     VNFNLNYRNHRKIVVIDGKVGFVGGYNVGDEYLGRNPKFGLWRDTHTKLTGDCVIDLN
                     MRFILDWRYTTKEDLDLEKYFIEEDTNSNHPSENIGIQIVSSGPDISELDEIKYGYLK
                     MIQKARKYIYIQSPYLILDSTFIDTLKIACLSGVDVRVMIPSKPDHPFVYWASYSYAG
                     ELLKFGAKIYTYGQNAFLHAKTIVIDDSICSIGTANMDIRSFELNFEVNAFMYSSKKA
                     LEQRIIFENDILNSKEITLDIYNSRSTYIKIKESISRLLSAVL"
BASE COUNT          595 a          189 c          239 g          543 t
ORIGIN      
        1 attatatata attacttatt ttaataaata tttgaaggag ggaattttat gtttgatata
       61 tcatttttcg aaatgtcaat atatgaaata gcagttacta caatatatat aattaatttc
      121 atggttatac taaaccttat ctttagagaa aaaagaaaca taaatacaac tttaacatgg
      181 cttcttatat tagtattgat tcctgctctt ggattcatac tttatatagc ttttggcaga
      241 aatatttcaa aaaacaatat gtttagattg aaagaaaagg atgataagat aatcaaaagt
      301 aatatactag atactcaggt taaactccaa tctacatctg aaattgattc agatatacat
      361 caacataagg atatgattta tgcattggct aattcaaata atgctcatta taccaataat
      421 aacgatgtgt ggatttatgc tgaatctagt caatttttta acagtcttct tgaagaactt
      481 aaaaaagcta aaaaatatat aaacatacaa ttttacattt tcaaagatga caaaatcgga
      541 actgaaataa tagacattct tatagacaaa gctaaagaag gtgttgaagt aagactttta
      601 tttgatgcag ttggtggtag aacactaaaa aatagtacac tttccagact taaagaaagt
      661 ggtgttaaag ttggaagctt cttcccatca ttcttgaaaa ttgtaaactt taacttaaac
      721 tatagaaatc accgaaaaat tgttgtcatt gatggtaagg taggatttgt aggtggatat
      781 aatgttggtg atgaatattt aggtagaaat ccaaaatttg gtctatggag agatacacat
      841 actaagctta ctggtgattg cgtaattgac ctaaatatga gattcatcct tgattggaga
      901 tatacaacta aagaagactt agatttagaa aaatacttta tagaagaaga tacaaattct
      961 aatcatcctt ctgaaaatat tgggattcag atagtatcaa gtggtccaga tatatcagaa
     1021 cttgatgaga ttaaatatgg ttaccttaaa atgattcaga aagctagaaa gtatatatac
     1081 atacagagtc cttatttaat acttgactca acttttatag atactttaaa aatagcttgt
     1141 ttatctggtg ttgatgtgag agtgatgatt ccttctaaac ctgaccatcc ctttgtatac
     1201 tgggcatcat attcatatgc tggagaactt ttaaagtttg gagctaaaat ctatacttat
     1261 ggtcaaaatg catttttaca tgctaagaca atagttatag atgactctat atgctctata
     1321 ggaacagcta atatggatat cagaagtttt gagcttaatt tcgaagttaa tgcatttatg
     1381 tactcttcaa aaaaagcatt ggaacaaaga attatatttg aaaatgatat tttaaattct
     1441 aaggaaataa cattagatat atataattct aggtctactt atataaaaat caaggaatca
     1501 atttcaagac tattatcagc agttttataa atcttttatt ttataaaaat tttacatttt
     1561 ggttta
//
LOCUS       DAELLV010000389         1549 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.391, whole genome shotgun sequence.
ACCESSION   DAELLV010000389 DAELLV010000000
VERSION     DAELLV010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.391"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            123..857
                     /locus_tag="KPD58_003657"
     CDS             123..857
                     /locus_tag="KPD58_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG0267361.1"
                     /translation="MKQNKYDDDRFFNKYSKMERSTNGLAGAGEWHVLKKMLPDFKGK
                     RVLDLGCGFGWHCQYAVENGAISAVGVDISEKMLKEARNKTQLDNINYICMPIEDINF
                     PKDSFDVVISSLAFHYIQSFEDICKNISNCLSNKGNFAFSVEHPIFTAYGSQEWYCDK
                     DGNNLHWPVDRYFEEGVRRSNFLGEEVIKYHKTLTTYLNTLMKTGFEILEIIEPQPEE
                     KLLDTIPDMKDELRRPMMLLVSAKKR"
     gene            complement(1000..1491)
                     /locus_tag="KPD58_003658"
     CDS             complement(1000..1491)
                     /locus_tag="KPD58_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBG0267362.1"
                     /translation="MNNQQQEVYKKLDELSIPYEAVNHPPVYTIEEMEELKTLHMEFV
                     VKNLFLRDAKGKEHFLVVLGKDKKADLKDIRQQIDSKPLSFASEERLQKYLKLTKGAV
                     TPFGILNDKEAVVKVVFDKDLLKMDKIGIHPNDNTATVFLKFEDMKKLIEENGNEIYY
                     VEV"
BASE COUNT          560 a          185 c          233 g          571 t
ORIGIN      
        1 ttatattatt aaaaaattta tttatagaaa cttttattat tttatataaa agtgataaaa
       61 acttatattt tgtgtttagt agagtgttgc taagtgaata tatttaaaat ggaggaaact
      121 ttatgaaaca aaataaatat gatgatgata gattttttaa taagtacagt aaaatggaac
      181 gttctacaaa tggattagca ggtgctggag aatggcatgt tttaaaaaaa atgttacctg
      241 atttcaaagg aaaaagagtt ttagatttag gatgtggatt tggatggcat tgtcaatatg
      301 cagttgagaa tggagcaatt tctgctgttg gagttgatat ttcagaaaaa atgttaaaag
      361 aagctagaaa caaaacgcaa cttgataata taaattacat atgtatgcct attgaagata
      421 ttaattttcc aaaagatagt tttgatgtag ttattagttc cttggcattt cattatattc
      481 aatcatttga agatatatgt aagaatataa gtaattgtct ttcaaataaa ggtaattttg
      541 cattttctgt tgaacatcct atatttactg catatggaag tcaagagtgg tactgtgata
      601 aagatggcaa taatctacac tggccagtag atagatattt tgaagaagga gtacgtaggt
      661 caaattttct aggagaagag gttataaaat atcataaaac attaacaact tatttaaata
      721 cgttaatgaa aacaggattt gaaatattag aaattattga acctcaacca gaagaaaaac
      781 tgttagatac aattcctgat atgaaagatg agctacgtag acctatgatg ttacttgtat
      841 ctgcaaaaaa aagataagta aatataaata atcattattg tagaaaaaaa gttgtagaaa
      901 ataattacac taatggagat gattatacca gtggtattaa ttacaccagt agtaaggagg
      961 caaaagcatt cgttgctttt gccatttttt aagttgcaat tatacttcta cgtagtatat
     1021 ttcgtttcca ttttcttcta taagcttttt catatcttca aattttaaaa atacagtagc
     1081 agtattatca tttggatgta tacctatttt atccattttt aataaatcct tatcaaatac
     1141 aacttttaca acagcctctt tatcatttaa tataccaaaa ggagtcacag ctcctttagt
     1201 aagtttcaag tatttttgaa gtctttcttc tgatgcaaaa ctaagaggtt tactatcaat
     1261 ttgttgacga atatctttta aatctgcttt tttgtcttta cctaatacaa ctaaaaaatg
     1321 ttcttttcct ttagcatcac gaagaaatag gttttttaca acaaattcca tatgtaatgt
     1381 ttttaattct tccatttctt caattgtata aactggtgga tgatttacag cctcataagg
     1441 tatgcttaac tcatctaatt ttttatatac ttcctgttgt tgattgttca tgatttcctc
     1501 ccattagaaa tttattttat atataaataa tattatcaca gaaaacttt
//
LOCUS       DAELLV010000390         1548 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.392, whole genome shotgun sequence.
ACCESSION   DAELLV010000390 DAELLV010000000
VERSION     DAELLV010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.392"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            240..1067
                     /locus_tag="KPD58_003659"
     CDS             240..1067
                     /locus_tag="KPD58_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HBG0267363.1"
                     /translation="MDLSKKVKILENASDGKSEFEILEFEKLEGASSPLMAMGLYFAR
                     EAGLKARQIKIKLNNSSIKTEAGALYYYKGNISSETKIGGVGGLFKKGISGALTNESM
                     MKPVYKGTGEVYLEPSFRHYLFMSLDNDSIIVDKGLFYCCSDTIEVKAFTQKNFSSAL
                     LGGEGFFQIELSGTGVIVLECVVPQSEIVEYELKNGEELKVDGNFAIARTSGVNFSVT
                     KSDKSLIGSALNGEGFLNTFTGNGTVWLAPTQVMYEKLSFGAMPTNDNMNNKVSKQK"
BASE COUNT          585 a          167 c          222 g          574 t
ORIGIN      
        1 ataattttaa catgcccata aaatataata cccacaaatg ataaataata ctcaaaatat
       61 ctattgaaat aactatacat ccttaaataa cttaacttaa atctaaacat ttcaaaaact
      121 tcttaaaaat atgtaaaaat ttgatgataa agtagcgatt ttttcaaaaa aaatgtaata
      181 tagagttata tcttataata ttttagagat aaattttatc atagtgggga gaaatttgta
      241 tggatttaag caaaaaagta aaaattttag aaaatgcatc agatggcaaa agcgaatttg
      301 agatattaga atttgaaaaa cttgagggag cttctagccc attaatggca atgggattat
      361 attttgctag agaagctggt ttgaaagcta gacaaattaa gataaaatta aataattcat
      421 caataaaaac agaagctggt gcattatact attataaagg aaatatatct tctgaaacta
      481 agattggtgg tgttggaggt ctttttaaaa aaggaatatc aggagcactt actaatgaat
      541 caatgatgaa acctgtatac aaaggtactg gagaagttta tttggaacct tcttttagac
      601 attatttatt tatgagtctt gataatgatt ctataattgt tgataaagga ttattctatt
      661 gttgctctga tacaatagaa gtaaaagcat ttacacaaaa gaacttctca tctgctttac
      721 ttggtggtga aggcttcttc caaatagaat taagtggtac tggtgtaatc gttctggaat
      781 gtgttgtacc tcaaagtgaa attgtagagt atgaattgaa aaatggtgaa gaattaaagg
      841 tagatggtaa ctttgcaata gcaagaacat ctggtgttaa tttttctgtt acaaaatcag
      901 ataaatcttt aattggttct gctttaaatg gtgaaggttt cttaaataca tttactggta
      961 atggtacagt ttggctagct ccaactcaag taatgtatga aaaactaagt tttggagcaa
     1021 tgcctactaa tgataatatg aataataaag tttctaagca aaaatagttg ttttataaat
     1081 atttattaaa tcattattga ttttataaag tcactataaa ttttattaga ttattattat
     1141 aaaatcagag tgtggaaatg tttatgtgta ttctttaaat aaaaatttcc acattctgta
     1201 aggttattat acttgatata taaaattcaa tttaggaaca tagtctaaca tttataaaca
     1261 tattaaggta atgatttctc ttagtaatta ttctcatatc ttattctttg attatattta
     1321 tcttattcct agcccttatg tcatattata tttcttatct tattatttat aattagcatt
     1381 aacttatcta aaatcttgta tttttttaat agtaaagttt acatctatat taaaaagttt
     1441 acatctatat taatttcata aaacaatcaa ggtagtcaag agattcaact ttatttgatt
     1501 ttaaaaattt aagtgaattt gttcttatac acaccttttt aaaattat
//
LOCUS       DAELLV010000391         1520 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.393, whole genome shotgun sequence.
ACCESSION   DAELLV010000391 DAELLV010000000
VERSION     DAELLV010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.393"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            627..>1520
                     /locus_tag="KPD58_003660"
     CDS             627..>1520
                     /locus_tag="KPD58_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HBG0267364.1"
                     /translation="MKINRLTEIIVILLNKKLVTAKELADRFEVSTRTIYRDIETLSM
                     SGVPVYMTKGKGGGISLIEEYSIDKAILSKKDKESLIVALKTLQATKYPEINSVVNKI
                     GSIFGEQNFSNWIEIDFTEWGSNFNEDDKFTKIKEAILRRNTINFNYVNSLSSQTNRT
                     VEPLKLMYKSKTWYLYGFCKLKDDFRIFRISRIRNLSIKDEVFSRKIIEEVCLNDSKV
                     IKENTITLKLRFKEKMLFRVFDDFNKDLITKNEDDTYDVITEFPIGEWIYGYILSFGD
                     NVEVLEPKDVRDNVITRLRELS"
BASE COUNT          687 a           93 c          270 g          470 t
ORIGIN      
        1 taataatata gagttgagaa atagtaaatt gaaattttaa aataaatgag tggaagtagc
       61 ttagtaaagt atggaaatga atattacaat agaaaataaa taatatgtaa agactttaaa
      121 ttaattttaa gtattaagta caaatactaa ttttgatata gaaattgaat aaagtaaatg
      181 ataataagca aatgaattaa gatgataata agtgaattga tatagaaata agtaaattaa
      241 gtaagacgat aacaagtgag ataataataa atgaattgac atagcaataa gtaaattaag
      301 taagacgata acaaatgaga tgataataag taaattgata tagaaataag taaattaagt
      361 aagatgataa caagtgaggt aataataagt gaattgatat agaaataagt aaattaagta
      421 agacgataac aaatgagatg ataataagta aattgatata gtaataaata aattaagatg
      481 ataatatcca aagtgagaaa gataatagaa agtaaataaa atcatgtaaa aataatttag
      541 ataagattct aagattttat ttatttaaaa attagtatga ttaatttaga ttaaataaat
      601 ttaataattt tagtaggtgg taatgaatga aaataaatag attaacagaa ataatagtaa
      661 ttctattaaa taaaaaatta gtaacagcta aagagttggc agatagattt gaagtttcta
      721 caagaaccat atatagagat atagaaactc tgtctatgtc gggagtacca gtatatatga
      781 cgaaaggcaa aggaggaggg atatctttaa tagaagaata ttctatagac aaggctatat
      841 tgtcaaaaaa agataaagag agtttaatag ttgcactgaa aacattacag gcgactaagt
      901 atccagaaat aaattcagta gtaaataaaa ttggctcaat atttggagag cagaattttt
      961 caaactggat tgaaatagac tttactgaat ggggaagtaa ttttaatgaa gatgataaat
     1021 ttacaaaaat aaaagaagct attttaaggc gtaatactat aaattttaat tatgtaaata
     1081 gtttgtcaag tcagacaaac agaactgtcg aaccactgaa gcttatgtat aagagtaaga
     1141 cttggtactt gtatggattt tgtaaactaa aagatgattt taggatattt agaattagta
     1201 ggataaggaa tttatctata aaggatgaag tattttcaag aaagataata gaagaagtat
     1261 gtttgaatga ttctaaagta attaaggaaa atactataac gttgaaattg aggtttaaag
     1321 aaaagatgct ttttagagta tttgatgatt ttaataagga tttaataact aaaaatgaag
     1381 atgatacata tgatgtaatt acagaatttc ctattggtga atggatttat ggatatattt
     1441 tatcatttgg tgacaatgtt gaagtacttg aacctaaaga tgttagggat aatgtaataa
     1501 ctagactaag ggagttgtct
//
LOCUS       DAELLV010000392         1512 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.394, whole genome shotgun sequence.
ACCESSION   DAELLV010000392 DAELLV010000000
VERSION     DAELLV010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.394"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(200..1360)
                     /locus_tag="KPD58_003661"
     CDS             complement(200..1360)
                     /locus_tag="KPD58_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HBG0267365.1"
                     /translation="MIETGFVYCSFLVVFVGVIFFMVDKLKWKVFDVINPMLLIYLGS
                     AILGSFGLFAQNEDVGTYQNIITVNFLPLMLVFLLIQCDLRKILKMGPKMLLSFFCAT
                     FTFMLGFVIAFIIFKNSMGADSWKILGAASGSWIGGSSNMIAAANALGVAEEGLSYAI
                     LMGSIGYTVWMSVCLMSVKFGPKFNKWTKCDTSFIDEVTSKLEKENKDNTAPTFVELM
                     ILLSVGFGVASFAQIVSKILPTSGILNETMWVVLVASTVSILLGMTKISKLKGSTLVG
                     NVFMYLLLAATGSKANFFGLSEAPVYIMFGLVVVLVHAISFIGLAKLFKLDLFTCEVG
                     SIANIGGVTTAPMIAGVHNPVLIPVGVLMGLLGNIIGTYCALMVTQILHMLA"
BASE COUNT          564 a          286 c          166 g          496 t
ORIGIN      
        1 catacagacg ttcgattcaa tataaaaatt aaatatatct cgtttttagt aaattttaca
       61 atattataca ctaaatatgt caaaacatct tcttgaaata gattatcttg tcaaacaaaa
      121 aagccatttc agtaaaagct tctgcttaaa ttgaaatgac ttttcttatt taaattatta
      181 attttatacc atatttttat taagctaaca tatgtaatat ttgagttacc ataagagcac
      241 aataagtccc tataatattt cctagaagcc ccataagtac acctactggt attaatacag
      301 gattgtgaac cccagcaatc attggagctg tagttactcc tcctatatta gctatacttc
      361 ctacttcaca agtaaataaa tcaagtttaa atagtttagc aagccctata aatgaaatag
      421 catgtacaag gactactacc aatccaaaca ttatgtaaac aggtgcttct gacaatccaa
      481 agaaatttgc tttactacct gtagctgcaa ggagtaaata cataaacaca tttcctacca
      541 gtgtactccc tttaagcttt gatatttttg tcatacccaa taatattgat actgtagatg
      601 caacaagtac tacccacata gtttcattaa gtatacctga agttggaagt atcttggata
      661 caatttgtgc aaaagacgca actccaaacc caacagaaag taaaatcata agttctacaa
      721 acgtaggtgc cgtattatct ttattttctt tttcaagttt tgatgtaacc tcatctataa
      781 aacttgtatc gcactttgtc catttgttaa atttaggtcc aaatttcaca gacattagac
      841 aaacagacat ccatactgta tatcctatag aacccataag tattgcataa ctaagtcctt
      901 cttctgcaac tcctagagca ttagctgctg caatcatatt tgatgagcca cctatccatg
      961 aaccagatgc tgccccaaga attttccatg aatctgcacc cattgaattt ttaaaaatta
     1021 tgaatgctat aacaaatcca agcataaatg taaatgtagc acagaaaaat gataataaca
     1081 ttttaggccc catctttaat atttttctta aatcacattg aattaacaaa aacactaaca
     1141 ttaatggaag gaagttcact gttattatgt tttgatacgt tccaacatct tcattctgtg
     1201 caaataaacc aaatgatcca agtatagctg aacctaagta tattaaaagc attggattaa
     1261 taacatcaaa taccttccat ttcaatttat caaccataaa aaaaattaca cctacgaata
     1321 caaccaagaa actacaatat acaaatccag tctcaatcac attatacaca ccctttcttt
     1381 tcctaattaa tttgattttt atgatttcta agtaaagact ttttcagata atagacaatt
     1441 aaaataaata cttttatcta tatcatacta attaaataca tatataccta actattgaca
     1501 tatattaata aa
//
LOCUS       DAELLV010000393         1511 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.395, whole genome shotgun sequence.
ACCESSION   DAELLV010000393 DAELLV010000000
VERSION     DAELLV010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.395"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..927
                     /locus_tag="KPD58_003662"
     CDS             <1..927
                     /locus_tag="KPD58_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG0267366.1"
                     /translation="IIAGFIYWYSSKKFNIKVMNFDKYIAILMIPLLLIILFMQSFEY
                     SSNINIDTSLGIVKLLLNTSITEEIQAFLFSIIGTICVFFSLFTFKKLIQALEDDKER
                     AIMNQQIHAQKNYIEEAKSRLSQTISFRHDFNNHLAIVNGLLKKDQILKAQDYLNKLE
                     KVTSDLSFSCNTQNIVIDALFNNKLSIAKQLGIEVDCEVIIPQSTNMNDFDLCIVFAN
                     AIDNAIKACRVVDKQNRYLKLSTKLEGGFFMIEIKNSYNPINNYTSGSGIGLLNIKEI
                     AKKYQGAISIDKTSDYFQINVLLVISLHCASI"
     gene            966..1451
                     /locus_tag="KPD58_003663"
     CDS             966..1451
                     /locus_tag="KPD58_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0267367.1"
                     /translation="MYLNRESLIYRNEEVTKMKVGNINGQAPITNGLDSCQSEVQGLE
                     QKKKSLQDEIEKVRSDKSIDGETAKLKIEALKKEMEEIENKIQELKGNKAKKTNKEEN
                     NVSEVNNNLLERKKEPSNNENVKQLYDVFERSNVDEQWDNTYRVLQDDKNLKVKFNRP
                     I"
BASE COUNT          636 a          156 c          236 g          483 t
ORIGIN      
        1 ataatagctg gatttatata ttggtatagt tctaaaaagt ttaatattaa ggtcatgaac
       61 tttgataaat atatagcaat tttgatgatt ccattgttgc ttataatatt atttatgcaa
      121 tcatttgaat atagttcaaa tataaatata gatacaagtc ttgggattgt aaagctgcta
      181 ttaaatactt ctataactga agaaattcaa gcttttcttt tttctataat tggaactata
      241 tgtgtatttt tttctctatt tacctttaag aaactaattc aagctctcga agatgacaag
      301 gagagggcaa ttatgaatca acagattcat gctcaaaaaa actatattga agaagcgaaa
      361 tcacgattat cacaaactat atcctttaga catgatttta ataatcattt agctattgtc
      421 aatggattgt tgaaaaaaga ccaaatttta aaagcacaag attatttaaa taagcttgaa
      481 aaagtaacaa gtgacctatc attttcttgt aatacacaga atattgttat tgatgctctt
      541 ttcaacaata aattaagtat agcaaaacaa ttaggcatag aggtagattg tgaagtaatt
      601 ataccccaaa gcactaacat gaacgatttt gacctatgta tagtgtttgc taatgctatt
      661 gacaatgcaa ttaaagcttg tagagttgta gataaacaaa ataggtattt aaagctttcc
      721 actaagttag agggtggatt ttttatgata gaaataaaaa atagctataa tccaataaat
      781 aattatacaa gtggctcagg tataggtctt ttaaatataa aagaaattgc taaaaaatat
      841 caaggggcta taagtataga taagacatca gattattttc aaataaatgt attgctggtt
      901 atttcattac attgcgctag tatttgataa caatttcatt gaaaatagta tttgtaagct
      961 atattatgta cttgaataga gaaagtttga tatatagaaa tgaggaggtt actaaaatga
     1021 aagttggaaa tataaatggt caagcaccaa ttacaaatgg tttagatagt tgtcaaagtg
     1081 aggtacaagg cttagaacaa aagaaaaaat cattacagga tgaaatagag aaagtaagaa
     1141 gtgataaaag tattgatggt gaaacagcca aattaaaaat agaagcttta aaaaaagaaa
     1201 tggaagagat tgaaaataaa attcaagagt taaaaggaaa taaagcaaaa aaaacaaata
     1261 aagaagaaaa taatgtttca gaagtaaata ataatctatt agagagaaaa aaagaacctt
     1321 ctaataatga aaatgtaaaa caattatatg atgtttttga aagaagcaat gtagacgaac
     1381 aatgggataa tacttatcgt gttctccaag atgataaaaa cttaaaagta aaatttaata
     1441 gacctatata gtttatttat aaaaatagtt aatattttaa ttatcaccac tttttagaag
     1501 aatacacact t
//
LOCUS       DAELLV010000394         1478 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.396, whole genome shotgun sequence.
ACCESSION   DAELLV010000394 DAELLV010000000
VERSION     DAELLV010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.396"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..>1478
                     /locus_tag="KPD58_003664"
     CDS             <1..>1478
                     /locus_tag="KPD58_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0267368.1"
                     /translation="QIDSKEIQSNLVKYSEEIISLDTGIKDKYKELFLKIEELDTILN
                     LSKDGILFTSKDGEINTYNSKVKDILDINEDIYGKYIEDIFVDSLKVLLSEKEILDKV
                     VVFNKKYINVNKKNIYNRDEKMGTYYSLQEITYIKKLEQNLTKKLREKGQIAKYTFKD
                     IKTNSPKMFECIDLAKKVSKSDLSILIRGESGTGKELIAQSIHNNSNRKNQPFIAVNC
                     AAVPENLLESQLFGYDKGTFTGGLKDGKQGLFELANNGTIFLDEIGDMPLELQTKLLR
                     VLQEKQIMPVGSHNVINIDVRIISATNKNLEQMIDNSQFREDLYYRLNTIPINIPPLR
                     ERKEDILIIMEDLINKKLVITPEAKKLIQNYMWKGNIRELQNVTSYLNIMCEDIVLEK
                     DLPPNLRSSDNKNTSLKLKYSKNDILNILEILILNKESDVGIGRGLILKALLDKNLQI
                     TEGKIKKIFEYLKKEELIICSSGRYGSKITQKGEDFYNKLKY"
BASE COUNT          681 a          148 c          221 g          428 t
ORIGIN      
        1 acaaattgat tcaaaggaaa tacaatctaa tctagttaaa tactcagaag aaataataag
       61 tttagatact ggtataaaag ataaatataa ggaattattt ttaaaaatag aagaattaga
      121 tacaatattg aatttatcaa aagatggtat attatttact tcaaaagatg gtgaaataaa
      181 tacatataat agtaaagtaa aagatatttt agacataaat gaggatatat atggaaaata
      241 cattgaggat atatttgtag atagtttaaa agttttattg agtgagaaag agatactaga
      301 taaagttgtc gtattcaata agaaatatat aaatgtaaat aaaaaaaaca tatacaatag
      361 agatgaaaaa atgggcacat attatagttt acaagaaata acatacataa aaaaattaga
      421 acaaaatctc actaaaaaat taagagaaaa aggacaaata gcaaaatata catttaagga
      481 tataaaaaca aatagtccta aaatgtttga atgtatagat ttagctaaga aagtttctaa
      541 atctgattta agcatactta tcagaggtga aagtggaact ggtaaagaac tcatagctca
      601 gtctatacat aacaactcaa atagaaaaaa tcaacccttt atagcagtaa attgtgcagc
      661 cgttcctgaa aatttattag aaagccagct attcggatat gacaagggaa cttttactgg
      721 aggtctaaaa gatggaaaac aaggattatt tgaacttgct aataatggaa caatattctt
      781 agatgaaata ggcgatatgc ctttagaatt acaaacaaaa ctacttagag ttcttcaaga
      841 gaaacagata atgcctgttg gttctcacaa tgtgattaat atagatgtta gaatcatatc
      901 tgctacaaat aagaatttag aacaaatgat tgataatagc caatttagag aggatttata
      961 ttatcgatta aatacaattc caataaatat acctcctcta agagaaagaa aagaagacat
     1021 attaattatt atggaagatt taattaataa gaaattagta atcacaccag aagcaaaaaa
     1081 acttatacaa aactatatgt ggaaaggtaa tataagagaa ctccaaaatg ttacatccta
     1141 tttaaacatt atgtgtgaag atattgtatt agagaaagat ttacctccaa acttaaggtc
     1201 ttcagataac aaaaatacat cattaaaact aaaatatagt aagaatgata tattgaatat
     1261 attagagata ctaattttaa ataaggaatc agatgttggt attggtagag gattaatttt
     1321 aaaagcacta ctcgataaga accttcaaat tacagaagga aaaattaaaa aaatatttga
     1381 atatctaaaa aaagaagaac ttataatttg tagttcagga agatatggaa gtaaaataac
     1441 tcaaaaagga gaagattttt acaataaatt aaaatata
//
LOCUS       DAELLV010000395         1464 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.397, whole genome shotgun sequence.
ACCESSION   DAELLV010000395 DAELLV010000000
VERSION     DAELLV010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.397"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            409..1116
                     /locus_tag="KPD58_003665"
     CDS             409..1116
                     /locus_tag="KPD58_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG0267369.1"
                     /translation="MIFTKSNKYDKDFLMKNMMGPNCIKILEELTSKIKLEKGMRILD
                     LGCGKGISSIFLAKEFDATVFATDLWIEPTENYKRFKEFKLDDKIFPIQAEAHELPYA
                     EGFFDAVISIDSYHYFGNKEGFLDNHISPLVKEGGILAMAMPGLKEDFVDCIPDELIP
                     FWQDNMNFHSITWWNKLWSESESVIVEKCEALNCHDEAWKDWINCDNSYAINDKKMME
                     VENGKYFNTISLIARTK"
BASE COUNT          609 a          132 c          258 g          465 t
ORIGIN      
        1 ttattaagta ttagaaaatg acttcttatt agagtaagta tctaaaatga tataaaaatt
       61 atgattactc attaaagtga ctattataga gtggataaga ttactcatta aagcgattac
      121 tgtggagtag ataaaattac tcactaaaat aactatcata gagtggataa gattactcat
      181 taaagcgatt gctgtggagt agataaaatt actcactaaa ataactatca cagtagataa
      241 gattattcat taaagttact atcacagagt ggataaaatt acacattaaa attgtctgtt
      301 aaagaatgaa taaaaaagag attgctcaat ataagaagca tataaaaaat aaaaaatcaa
      361 acgtataaaa tttaataaat aataactgat agggaaagga aagatagaat gatatttaca
      421 aaaagtaata aatacgataa ggactttctc atgaaaaata tgatgggacc taattgtata
      481 aaaatattgg aagaattaac aagtaagata aaactagaaa agggaatgcg tattttagac
      541 ttaggttgtg gtaaaggtat atcttcgata tttttagcaa aggaatttga tgctactgta
      601 tttgcaacag atttatggat agaaccaact gaaaactata aaagatttaa agagtttaaa
      661 ttggatgata aaatatttcc aatacaggca gaggcacatg aactacctta tgcagaaggt
      721 ttctttgatg cggtgataag tatcgattct tatcactatt ttggaaacaa agaaggattt
      781 ttagacaatc atatatcacc tcttgtaaaa gaggggggaa tacttgctat ggcaatgcct
      841 ggattaaaag aagatttcgt ggattgtata ccagatgaac tcattccatt ttggcaagat
      901 aatatgaact tccattcaat tacatggtgg aataaacttt ggtctgaatc ggagtctgtg
      961 attgtagaga aatgtgaagc tttaaattgt catgatgaag cttggaagga ttggataaat
     1021 tgtgataatt catatgctat aaatgacaaa aaaatgatgg aagtagagaa tggaaagtat
     1081 tttaatacaa tttctttgat agcgagaact aaataattgt gatgaattta taaaagtata
     1141 agaagcatag aaatataagt tgaatgagag tataagaagc atagaaatat aagttgaatt
     1201 aaagtagaag gtaaataaaa atgtaagtta aataaagagt ttatacaaaa atgatttttt
     1261 agattattta tacccaatcg agttgcttag gtcagttggg tatttttatt ttttaaagtt
     1321 aaattgcaaa tactattgtg tgatatatat tttaatacta aatgtttgtt ttataagaaa
     1381 taaactaata gaaaaaatag ttttaacaat gaaagcaaaa aaggtattga tgaagattaa
     1441 aaattttata gtaccatttt atat
//
LOCUS       DAELLV010000396         1447 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.398, whole genome shotgun sequence.
ACCESSION   DAELLV010000396 DAELLV010000000
VERSION     DAELLV010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.398"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            502..>1447
                     /gene="blaCDD"
                     /locus_tag="KPD58_003666"
     CDS             502..>1447
                     /gene="blaCDD"
                     /locus_tag="KPD58_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HBG0267370.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYLGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNDKNKKEKINMVDYSDCFEDISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLINKVDAKYVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSV"
BASE COUNT          630 a          124 c          224 g          469 t
ORIGIN      
        1 taacctgtca ataaaattca ttaataaatc aataaaaaca atatttgtaa aagtaaaagc
       61 tataaattat atattaaatt ttataattta tagcttttat aatactgctt atcttaatcc
      121 tcccaatagc aaaataattt taaacatgcc atattttata aatgtaataa caattataca
      181 attaaaataa ctttcccaaa gtaacttgac atttaaaact tactattgta atatttaaat
      241 attacaatag taagttttaa atgtggaatc aattattata tcacaaatta aaaggaggac
      301 aggatgttat taatagttac ctaattatct tcgcaatagg tttaatcaac ataattgtac
      361 taggtgaaga aaatataaga gatacagcta ttcatttagt cttgtttctt ataagtagaa
      421 cctagattgg agtatattac aaataatgta tactatttta atagtaggtt ataaatttaa
      481 atatgggaga tgatataaat tatgaaaaga aaaaagaatt ttatttggat agctatctta
      541 ttggttggtg tagttatggt aatgtattat ttagggaaaa aacataacga tataaatcaa
      601 aaaactgata agaattatat aaaatctgaa ttaaataagt ctgaattaaa taaatccaaa
      661 attgaaaaca atgataaaaa caaaaaagaa aaaattaata tggttgacta tagtgattgt
      721 tttgaagata ttagtggagg agctattttt tataatacta aaaataaaga atataatata
      781 tataacaaag aattaattga aacaagaagg tcaccatgtt ctacttttaa aatagtttca
      841 actttaattg gtttagaaaa aggggtaata aattcaaaag aatcagttat gggatatgat
      901 ggtacagagt atccaaataa aaattggaat aaaaatctta gtttagaaga ggcatttaag
      961 gagtcttgtg tttggtacta caaaaaattg ataaataaag ttgatgctaa atatgtccaa
     1021 aacattttag atgatttaaa gtatgggaat tgcgatatta gcgaatggga aggagattta
     1081 aaaaatggaa agggacattt aaatggattt tggttagaat cttcattaca aatatctcca
     1141 aaagaacaag tgcaaacaat ggcaaaaata tttgagggag atacaaactt taaaaaagag
     1201 catattaata ttttaagaga tattatgaaa atagatgtaa atgataaaaa cataaatgta
     1261 tatggaaaaa ctggaacagg atttgatgaa aaaaataaac gtgtggatgc atggtttgtt
     1321 ggtatgctag agcgtgaagg tgacacctat tattttgcta ttaaatcaga tgattccaat
     1381 aaagaaatta cagggccaaa ggtaaaggaa attgcaatta atataattaa gaaatattat
     1441 agtgtaa
//
LOCUS       DAELLV010000397         1417 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.399, whole genome shotgun sequence.
ACCESSION   DAELLV010000397 DAELLV010000000
VERSION     DAELLV010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.399"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(202..1140)
                     /gene="pdaA"
                     /locus_tag="KPD58_003667"
     CDS             complement(202..1140)
                     /gene="pdaA"
                     /locus_tag="KPD58_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HBG0267371.1"
                     /translation="MFRKVLHYLTLLSISIFFIVGCSNSQNNQNENQNKETQLQEDKE
                     KIDSGKDTSNVIVSDGTDKPSKATTNNDDNKLDVSSLDNTTLDWFYIPNNKHKTPEVN
                     TDIEFKFSDYDALYNGPTKDGQKTLYLTFDEGYENGYTTKILDTLKQNQVKAVFFVTT
                     PYIKENKDLVKRMVSEGHIVGNHSKTHPSMPTKTSNLKNFNDELYDVEKLYKDVTGKD
                     MVKFFRPPMGKYSEKSLAMTKNLGYKTVFWSFAYRDWDTDKQPSHEEATQKIMDNLHD
                     GSILLLHAVSKTSTEILNDFISNARKLGYEFELLEY"
BASE COUNT          479 a          193 c          176 g          569 t
ORIGIN      
        1 catctaaaat actgttatct atatttaata tcttgccaaa ttttttgatg ttttattgtc
       61 ctgtcccacc aacatatata agctatcaca agtttttacc tatactatca ccaaacaaaa
      121 attctaattt taatttatta ttttataacc tatattatat taactatatt agtttgtaga
      181 ctttagatgt aattataaat attaatactc caagagttca aattcatatc ctagttttct
      241 tgcatttgat ataaaatcat ttaaaatttc tgtactagtt ttggaaactg catgtagtaa
      301 caagatagaa ccatcatgta gattatccat tattttttgc gttgcttctt catgtgatgg
      361 ttgtttatca gtatcccagt ctctgtatgc aaaactccaa aatacagtct tgtatcctaa
      421 atttttagtc attgccaagc ttttttctga gtattttccc ataggaggtc taaaaaattt
      481 aaccatatct ttcccagtta catctttata taacttttca acatcatata attcgtcatt
      541 aaagtttttt agattgcttg tttttgttgg catagatgga tgggttttac tatgatttcc
      601 aactatatgt ccctcagaaa ccattctttt tactaaatct ttattttctt ttatatatgg
      661 agttgtaaca aaaaaaactg ctttaacctg gttttgtttt agagtgtcga gtatctttgt
      721 agtgtatcca ttttcataac cttcatcaaa tgtaagatat agtgtttttt gaccatcttt
      781 tgtaggtcca ttgtataggg catcatagtc agaaaattta aattcaatgt ccgtatttac
      841 ttctggtgtt ttatgcttat tgtttggtat ataaaaccaa tcaagagtag tgttatctaa
      901 agaagataca tctaatttat tatcatcatt attagtagtg gcttttgatg gtttatctgt
      961 tccgtctgaa actattacat tggaagtatc tttaccacta tctatttttt ccttatcttc
     1021 ttgtaactgt gtttccttat tttgattttc gttttgattg ttttgtgaat ttgaacatcc
     1081 aacaataaaa aaaatagaaa tagaaagtag tgttaaataa tgcaatactt ttctaaacat
     1141 tttgaagctc ctttctaaat taataataat tgcatataat aataattata tgattaatta
     1201 caattgcata tagtattatt ataattggaa attactatta ataatagtta tatctttaaa
     1261 aataaagtca caatagtatg acaaagggtg aataatcaat aaatttaaaa aaaaatgaaa
     1321 aaaatagttg accatattat gagaagatgt tattataata aatgtgctca acaagaagta
     1381 acaaaataac aagcacaaag aagttaattg aatgaaa
//
LOCUS       DAELLV010000398         1414 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.400, whole genome shotgun sequence.
ACCESSION   DAELLV010000398 DAELLV010000000
VERSION     DAELLV010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.400"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(114..1154)
                     /locus_tag="KPD58_003668"
     CDS             complement(114..1154)
                     /locus_tag="KPD58_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HBG0267372.1"
                     /translation="MNLNLTNTINLMKKYLDIPSPAGYTENAVLEIKKDFENLGLDTI
                     LTKKGALIATLPGEDDSNQITISAHMDTLGAIVKEISSDGTLKYHKVGGGCWAAIEGE
                     NCTVITRKGKKIRGSVVFKYASTHIYGQTKAATERNEETMIIRLDEKVFTKQDVLDLG
                     ISVGDFVCLDHRFEATESGFIKSRYIDNKSAVAMVLEICRYFKENNLTPKHTTNFYIS
                     NYEEIGHGVSKCLPEKTKEFVAIDIAPTGPSQTSSEHGVTIVAKDNLSLYDFNLRNKL
                     VDVAEDNNLDYAVDVFTFYGSDASEAIRWGEDVQIACVGPGTNNSHHYERTHIEAIEN
                     TLKLLINYMLKE"
BASE COUNT          515 a          203 c          154 g          542 t
ORIGIN      
        1 atataatagt agtaaattga aattaagtct atagaaaaac caatttttgt ataaaaataa
       61 aaattaaagt taattatttg ttaagaattg actttggtaa aatatataat taattattct
      121 ttaagcatat aatttataag taattttagt gtattttcaa tagcttctat atgtgttctt
      181 tcataatggt gactattgtt tgttccagga ccaacacatg ctatttgaac atcttctccc
      241 caacgaatag cttctgaagc atctgaacca tagaaagtaa atacatcgac agcgtagtct
      301 aaattattgt cttctgcaac atcgacaagc ttgtttctta aattaaaatc atataatgat
      361 aaattatctt ttgcaactat agttacacca tgttcactag atgtttgact tggtccagta
      421 ggtgctatat ctattgctac aaattctttt gttttttcag gtaaacattt agaaacacca
      481 tgtccaattt cttcataatt acttatataa aagttagtag tatgcttagg tgttaaattg
      541 ttttctttaa aatatctaca tatttcaaga accattgcta cagctgattt attatctata
      601 tatctagatt ttataaaacc agattctgta gcctcaaatc tatggtctag gcatacaaaa
      661 tccccaacac ttattcctaa atctaataca tcttgctttg taaatacttt ttcatcaagt
      721 ctaataatca ttgtttcttc atttctttca gtggcagctt ttgtttgacc atatatatga
      781 gtagaagcat acttaaatac aacacttcct ctgatttttt taccttttct agttataaca
      841 gtacagtttt ctccctctat agccgcccaa catccaccac caactttatg gtatttaaga
      901 gtaccatcag atgatatttc ttttactata gctccaagtg tatccatatg tgcagatatt
      961 gttatttgat ttgaatcatc ttcaccagga agagtagcta taagtgcacc tttttttgta
     1021 agaatagtgt caagacctaa attttcaaaa tcttttttga tttctaaaac tgcattttca
     1081 gtatatccag ctgggcttgg aatgtccaaa tattttttca ttaaatttat agtattagtt
     1141 aaatttaaat tcatattgtt tcctcctaaa taaatatatt tatacaaatt ataacataaa
     1201 tacaaatgtt ctgaaaacta aaattaaaag aatagaatct aaacatattt aaaaaaaaag
     1261 tatataaaat aaatttgatt tatattatat agatttaaag taaaaatagt tttatttaat
     1321 aattaataaa aataaataca aaacaaaata aataaataat aaaatattta gtttaattat
     1381 ctttacaaat aaaaatttta gtaataaaat aatt
//
LOCUS       DAELLV010000399         1392 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.401, whole genome shotgun sequence.
ACCESSION   DAELLV010000399 DAELLV010000000
VERSION     DAELLV010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.401"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..476
                     /locus_tag="KPD58_003669"
     CDS             <1..476
                     /locus_tag="KPD58_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0267373.1"
                     /translation="SNTLKAPKNLRDILKVNVNSKIFDVKPMKNLINKGSNLKLCNLI
                     VFGCIEVEIDYSCKNRSNFKTVNKSNDIKKDKYYKNDIQNNNENINDCKKQISFGDNK
                     VKKITKTFGFSCFICSPVGIIYEDMKNINIKIEHTSINELNPEELFVNTSLLLYY"
     gene            766..1308
                     /locus_tag="KPD58_003670"
     CDS             766..1308
                     /locus_tag="KPD58_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG0267374.1"
                     /translation="MKICITVGHSILKGGKSTGVNGIVDEYNYNKKLAPMLAEMLISQ
                     GNTVDVIICPERHFMSEREEFFYRVPKVNSGKYDLLVELHLNKADGTQCGTEVLYYGT
                     EGLEYAKRVSNRLGELFENRGAKKRENLYILRNTNPVAIQIESFFCDNVNDCNKANES
                     GYDYIARLITEGILNKDINM"
BASE COUNT          621 a          103 c          208 g          460 t
ORIGIN      
        1 taagtaatac tttaaaagct cctaaaaatc taagagatat attaaaagta aatgtaaata
       61 gtaaaatatt tgatgttaaa cctatgaaaa atcttataaa caaaggtagt aatttgaaat
      121 tatgtaactt gatagttttt gggtgtatag aagttgaaat agactatagt tgtaaaaata
      181 ggagtaattt taaaacagta aataagtcta atgacattaa aaaagataag tattataaaa
      241 atgatataca aaataacaat gaaaatataa acgattgtaa aaagcaaata agttttggtg
      301 ataataaggt aaaaaaaata acaaaaactt ttggattttc ttgtttcatt tgttctccag
      361 ttggaattat ttatgaagat atgaaaaata taaatataaa aattgaacat acatcaataa
      421 atgaattaaa tccagaagaa ttatttgtaa atacatcact tttattgtat tattaagtat
      481 tttaaagcta aaacttaaac ttattaaaat agcatttggt taaaggaaaa tagatattga
      541 tatatttatc atatatataa atgattaatt aaacaattat aaataataag tatagtataa
      601 tatttaaaaa atatagattt atgataaaat aagacaaata tgttaaaaaa atgataatca
      661 cggagaactt ttaccttctt caattaatat attattatta gtaataactt taaaattata
      721 ttactattat ataaaaattg gcttaaaaga aggtgaaaaa agtaaatgaa aatatgcata
      781 acagtaggtc atagtatact aaaaggtggt aaaagtacag gtgtaaatgg tatagttgat
      841 gaatacaatt ataataaaaa attagctcct atgttagctg agatgcttat aagtcaaggt
      901 aatacagtag atgtaataat ttgtccagaa aggcacttta tgtcagaaag agaagaattt
      961 ttctatagag ttccaaaagt aaacagtggt aaatacgatt tattagtaga gctacattta
     1021 aataaagctg atggaacaca atgtggtaca gaagtgcttt actatggcac tgaaggatta
     1081 gaatatgcta aaagagtatc taatagactt ggagaattat ttgaaaatag aggagctaaa
     1141 aaaagagaaa atttatatat attgagaaat acaaatccag ttgcaataca gattgaaagt
     1201 tttttctgtg ataatgtgaa tgattgtaac aaagcaaatg aatcaggtta tgattatata
     1261 gcacgattga ttacagaagg tatattgaat aaagatataa atatgtgaga ttatggtgat
     1321 ttaggtattg attaatacct tttaatatta gatataaata aaaatacaat gtattagtat
     1381 aatttaaact tt
//
LOCUS       DAELLV010000400         1390 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.402, whole genome shotgun sequence.
ACCESSION   DAELLV010000400 DAELLV010000000
VERSION     DAELLV010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.402"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..394
                     /locus_tag="KPD58_003671"
     CDS             <1..394
                     /locus_tag="KPD58_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG0267375.1"
                     /translation="KKIEHVVITNNNIENYINFYKKFGFSSKEMSKNYKLNARNVKIN
                     LNIKGYKFLPYVKNSKASRDNLCFKINTNIDEPKKYLEQNEIQIERGIVECTGVFGNI
                     KSIYLKKTNGNLIELSNHRITESKPWKI"
     gene            382..1320
                     /locus_tag="KPD58_003672"
     CDS             382..1320
                     /locus_tag="KPD58_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBG0267376.1"
                     /translation="MENLILSINVVLPLFLTMSLGYVLKKLGLFDTHTLNKMNNVCFK
                     SFLPLLLFFNIYNTDLDGSLNFKLMAVAVIIIFSIFLILVFIIPKIEKDNKTRGALIQ
                     GVFRSNFVIFGIPVTISLFGDKSIGVTSLLIAVIVPMFNVLSVVILEIFRGGKVNIKH
                     MIKSVLTNPLIIASFIGLTMLILNINIPTFLHKTIGDISKIATPLSLIILGGSFSFSA
                     IKGHINPIILGVCGKLILVPAIFLPISIFLGFRNVELASLMIMLSAPTAVSSFTMAQQ
                     MDSDSELAGQIVVFTSGFSIITVFLIIFILKQNNLI"
BASE COUNT          506 a          171 c          188 g          525 t
ORIGIN      
        1 aaaaaaaata gaacatgtgg tcattactaa taataatata gaaaattata tcaattttta
       61 taaaaaattt ggtttttcat ctaaagaaat gagcaaaaat tataaactta atgctagaaa
      121 tgttaagata aatcttaaca taaaaggtta taaattttta ccttatgtta aaaattcaaa
      181 agcaagtaga gataacttat gttttaaaat aaacacaaat attgatgagc caaaaaaata
      241 cttagaacaa aatgagattc aaattgagcg aggaattgta gaatgtacag gtgtctttgg
      301 taatataaaa tctatttatt tgaaaaaaac taatggaaat ttaattgaat tatcaaatca
      361 tagaatcacg gagagtaaac cttggaaaat ttaatcctat caataaatgt tgttcttcct
      421 ttgtttctca caatgtcact tggatatgtt ctaaaaaaat taggtctatt tgatactcat
      481 actttaaata aaatgaataa tgtctgcttc aagtctttct tgccattatt attatttttt
      541 aatatataca atacagattt agatggttct cttaatttta agcttatggc tgtagctgtt
      601 ataattattt tttcaatctt cttaatctta gtctttatta ttcctaaaat tgaaaaagat
      661 aataagacaa ggggtgcatt aatacaaggt gtatttcgta gcaatttcgt tatatttggt
      721 attcctgtga caatttcact atttggcgat aagagtattg gagtcacatc tttattaatt
      781 gcagttattg tacctatgtt taatgtatta tctgttgtaa ttcttgaaat ttttagaggt
      841 ggcaaagtaa atataaaaca tatgattaaa agcgtcttaa caaatccact cataatcgcc
      901 tcttttatag gattgacaat gctaatttta aatattaata tacctacatt tttacacaaa
      961 actattggtg atatttctaa aatagctact cctttatcac ttataattct aggcggctct
     1021 tttagttttt ctgctataaa ggggcatata aaccccatta tattgggcgt atgtggaaaa
     1081 cttatacttg tacctgctat atttttacca attagcattt ttcttggatt tagaaatgta
     1141 gaattagctt cacttatgat aatgttatct gctccaactg cagtttcttc atttacaatg
     1201 gcacaacaga tggattctga cagtgaatta gctggacaaa ttgtagtatt tacttctgga
     1261 ttttctataa ttacagtatt tttaataata tttattttaa agcagaataa tcttatttaa
     1321 tgatatttga taacacaaat tggtattatg agaaacatat ataaaattga tttaactgcg
     1381 taaaaacata
//
LOCUS       DAELLV010000401         1383 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.403, whole genome shotgun sequence.
ACCESSION   DAELLV010000401 DAELLV010000000
VERSION     DAELLV010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.403"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(176..487)
                     /locus_tag="KPD58_003673"
     CDS             complement(176..487)
                     /locus_tag="KPD58_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0267377.1"
                     /translation="MKKIIKSFTFWFFLIALFEIYMHQIGQDSKSIVLIGLNPILSII
                     SRVDSFFVFMDSGMQIPCRTITGSISIYWYIASILSFLIYGIILDLIRIVISKIGNKT
                     K"
     gene            complement(543..1136)
                     /locus_tag="KPD58_003674"
     CDS             complement(543..1136)
                     /locus_tag="KPD58_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG0267378.1"
                     /translation="MISDSISKRRSIRKYKNQSISHETIEKIIEAGINAPSSKNRQPW
                     RFVVITEKEKESMLKAMSKGIQNEINDNGLLPGSRQHIAGANYTVEIMKQAPVTIFIL
                     NILGKSPLEKLSPEERFYEMANMQSIGAAIQNMSLTAVELGLGSLWICDVYFAYRELC
                     EWLNTDSQLVAAISLGYPDEEPSRRPRLQLSDVTEWR"
BASE COUNT          486 a          195 c          178 g          524 t
ORIGIN      
        1 atttaaagaa atacaataca ttaattttta agtaacatta aattaatgta agtaaaataa
       61 tgttcatatg aagatttaaa ggtgcatttt ctgaagctaa gtatatgttg tagagtgtta
      121 ggtctctcta tcctgtgaag tttaatagct caagaaaatg cactatttat gatgattatt
      181 ttgttttatt tccaattttg gaaataacta ttctaattaa atctaaaatt ataccataaa
      241 taagaaaaga aagtatagat gctatgtacc aataaatcga aattgaccct gtaattgttc
      301 tacatgggat ttgcatacct gaatccataa aaacaaaaaa actatcaact ctggaaataa
      361 tagacaaaat aggatttaaa cctattagaa caatactttt tgagtcttga ccaatttgat
      421 gcatataaat ctcaaataat gcaattaaaa aaaaccaaaa tgtaaaagat ttaataattt
      481 ttttcatcaa aatacctcac ataatataaa ttctgtttgc actatttgct tactacaact
      541 atttatctcc attcagtcac atcacttaat tgtaatctag gtcttcttga tggttcttca
      601 tcaggataac ccaaagatat tgctgccact aattgactat ctgtatttaa ccattcacat
      661 aattcacgat atgcaaagta tacatcacaa atccaaagac tacctagacc taattccaca
      721 gctgtaagcg acatattctg aattgcagct ccaattgatt gcatgtttgc catttcatag
      781 aatcgttctt caggggatag cttttctaaa ggtgatttac ctaatatatt taaaataaaa
      841 attgttactg gagcttgttt catgatttca actgtgtaat ttgcaccagc gatatgttga
      901 cgacttcctg gaagaagccc attatcatta atttcatttt gaataccttt gctcatggct
      961 ttaagcatag attctttttc cttttcagtg atgacaacaa aacgccatgg ttgcctgttt
     1021 ttagatgaag gtgcatttat tccagcttca attattttct ctatagtttc atgtgaaatg
     1081 ctttgatttt tatattttct gatacttcgt ctttttgata tagaatcact tatcatttta
     1141 agtaacctcc gtttattttt tggaaaaatc tatttaattt ttattttcta ttttaattat
     1201 tttagcatta atcagaaaaa aagtcattga ttttagtata caattattaa tgattatatt
     1261 aagaattgta tcaaaaagta tgtgattata ctaagggatt atattaaaca ttatatttaa
     1321 aattgtatga aaggttgtat ttaatattat attgagagat atatatatta ggatttatat
     1381 ata
//
LOCUS       DAELLV010000402         1382 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.404, whole genome shotgun sequence.
ACCESSION   DAELLV010000402 DAELLV010000000
VERSION     DAELLV010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.404"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            111..1004
                     /locus_tag="KPD58_003675"
     CDS             111..1004
                     /locus_tag="KPD58_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBG0267379.1"
                     /translation="MKNKKFIVIILIISILIGVLVKEYSSREIKKDDINVSKYIKYAD
                     LASKNNAQVNWKYVASIVAVLNKNNLKNVKDSQIQEVSDLFVKNFSKNNKINKLSDIL
                     DELEFSNRQKRLVDNYIDNLKDYGIKPERLKSDTKYMKFIDEIKTEAIQNYKDYKILP
                     SITIAQAIIESSWGKSTLAKQYSNLFGIKADAYWKGKSVTLETKEHLDTIIDDKFRIY
                     DDKNESIKDHAKFLATNKRYKNNGVFDAKTYIYQAKALEKAGYSTAKDENGNSIYAAR
                     LIELIQQYNLQLIDSEIQSEM"
BASE COUNT          594 a          134 c          202 g          452 t
ORIGIN      
        1 attaaatata tttcataaat cttcataaaa tcttcaaaat ttcgtaaaga atatgtcaca
       61 aaagtaaggt atattatatt cagcagatag tataagggag gacaatattt atgaagaata
      121 aaaaattcat tgttataata ctgataatat caattttaat aggtgtatta gtgaaggaat
      181 attcatctag agagataaaa aaggatgata taaatgtcag taagtatata aaatatgctg
      241 atttagcaag taaaaataat gcacaagtta actggaagta tgtagcttcc attgttgctg
      301 tgttaaataa aaataactta aaaaacgtta aggattcaca gatacaagaa gtatcagatt
      361 tatttgttaa gaatttttct aaaaataata aaataaataa gttaagtgat attttggatg
      421 agttagaatt tagtaataga caaaaaagat tggttgataa ttatatagac aatcttaagg
      481 attatggtat aaaaccagaa agattgaagt ctgatacaaa atatatgaag tttattgatg
      541 aaattaaaac agaagcaata caaaattata aggattataa aatacttcca tctataacta
      601 ttgcacaagc tataatagaa tctagctggg gcaagtcaac tttggcaaaa caatatagca
      661 atctatttgg tataaaggct gacgcttatt ggaaaggtaa gtcagtgact ttagaaacga
      721 aagaacattt agatacaatt atagatgata aatttagaat atatgatgat aaaaatgaat
      781 ctataaaaga ccatgcaaaa tttttagcca caaataaaag gtataaaaac aatggtgtat
      841 ttgatgcaaa aacgtatata tatcaagcta aagcattaga aaaggcaggt tatagtactg
      901 cgaaggatga aaatggcaat agtatatatg cagctcgtct aatagaatta attcaacagt
      961 ataatcttca attaatagac agtgaaattc aaagtgaaat gtaactttga ttaaattttt
     1021 attgataagg ctatgatttt taaaatcata gccttatttt gacatgcttt tacttgtaat
     1081 tattaaatcg atatcattat ctttcaaaat ttactttata ctgtaaatca aattatttaa
     1141 gttaaaactt ggaattattt ataaaaggat tatttacttt atctaaattc tgcacttaaa
     1201 gaacatcttt tcccttaaga ctgaccgtta gtctataaaa attataatta cttataaaaa
     1261 agtgaatata tatataaagt tttcagaaag acttttactt gtaaattgaa atcaatgaag
     1321 taaaaaagga ttctttctta aaagtaaata acaactagat agtgaataat aaaaaatggt
     1381 aa
//
LOCUS       DAELLV010000403         1336 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.405, whole genome shotgun sequence.
ACCESSION   DAELLV010000403 DAELLV010000000
VERSION     DAELLV010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.405"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            27..101
                     /locus_tag="KPD58_003676"
     tRNA            27..101
                     /locus_tag="KPD58_003676"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Thr,seq:tgt)
     gene            116..200
                     /locus_tag="KPD58_003677"
     tRNA            116..200
                     /locus_tag="KPD58_003677"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Tyr,seq:gta)
     gene            210..293
                     /locus_tag="KPD58_003678"
     tRNA            210..293
                     /locus_tag="KPD58_003678"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:244..246,aa:Leu,seq:tag)
     gene            323..399
                     /locus_tag="KPD58_003679"
     tRNA            323..399
                     /locus_tag="KPD58_003679"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:357..359,aa:Arg,seq:tct)
     gene            407..482
                     /locus_tag="KPD58_003680"
     tRNA            407..482
                     /locus_tag="KPD58_003680"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:440..442,aa:Gln,seq:ttg)
     gene            570..658
                     /locus_tag="KPD58_003681"
     tRNA            570..658
                     /locus_tag="KPD58_003681"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:604..606,aa:Ser,seq:tga)
     gene            662..737
                     /locus_tag="KPD58_003682"
     tRNA            662..737
                     /locus_tag="KPD58_003682"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:695..697,aa:Phe,seq:gaa)
     gene            744..817
                     /locus_tag="KPD58_003683"
     tRNA            744..817
                     /locus_tag="KPD58_003683"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:778..780,aa:Met,seq:cat)
     gene            832..908
                     /locus_tag="KPD58_003684"
     tRNA            832..908
                     /locus_tag="KPD58_003684"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:866..868,aa:Ile,seq:cat)
     gene            932..1008
                     /locus_tag="KPD58_003685"
     tRNA            932..1008
                     /locus_tag="KPD58_003685"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:966..968,aa:Pro,seq:tgg)
     gene            1016..1092
                     /locus_tag="KPD58_003686"
     tRNA            1016..1092
                     /locus_tag="KPD58_003686"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1050..1052,aa:His,seq:gtg)
     gene            1101..1176
                     /locus_tag="KPD58_003687"
     tRNA            1101..1176
                     /locus_tag="KPD58_003687"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1134..1136,aa:Lys,seq:ttt)
     gene            1184..1257
                     /locus_tag="KPD58_003688"
     tRNA            1184..1257
                     /locus_tag="KPD58_003688"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1216..1218,aa:Cys,seq:gca)
     gene            1264..1335
                     /locus_tag="KPD58_003689"
     tRNA            1264..1335
                     /locus_tag="KPD58_003689"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1296..1298,aa:Asn,seq:gtt)
BASE COUNT          321 a          309 c          355 g          351 t
ORIGIN      
        1 ccccttccag gtcgccaaaa taaaatgctg gtgtggctca acggtagagc agctgacttg
       61 taatcagcag gttgtaggtt cgattcctat caccagctcc atgtaaaatt aatacggagg
      121 atttcccgag cggccaaagg gggcagactg taaatctgtt gtcatcgact tcggtggttc
      181 gaatccacca tcctccacca aaaaaatgcg cggatgtggc ggaattggca gacgcactag
      241 acttaggatc tagcgccatt ggcgtggggg ttcgactccc ttcatccgca ccatttttgt
      301 ttgatataca tatataaatt gtgggtgcat agctcagctg gatagagcaa cgcccttcta
      361 aggcgtgtgt ccggggttcg aatccctgtg cgctcaccac atatattggg gattagccaa
      421 gtcggtaagg cacatgactt tgactcatgt atgcgtaggt tcgagtcctg catccccagc
      481 caaatgatgt attactaagc tatatttgaa ttattttatt ttaaatatag ttaggttcaa
      541 ttttagaata agtttaagta tccgaatgtg gagaggtgtc cgagtggttt aaggagctgg
      601 tcttgaaaac cagtgacttc gaaaggggcc gtgggttcga atcccaccct ctccgccaaa
      661 tgcccagata gctcagtcgg tagagcaggg gactgaaaat ccccgtgtcg gtggttcgat
      721 tccgcctctg ggcaccaaaa tatggcggta tagcttagtt ggctagagcg ttcggttcat
      781 acccgaaagg tcataggttc gactcctatt accgctacta aaatttaatg tgggccatta
      841 gctcagttgg ttagagcgcc cggctcataa ccggtaggtc tggggttcga gtccctgatg
      901 gcccaccaca ttaaattaat taatataaat tcgaggtgta gcgcagtttg gtagcgcaca
      961 tggtttggga ccatggggcc gggggttcga gtcccttcac ctcgaccaat ataaggtggg
     1021 tatagctcag ttggttagag cgccagattg tggctctgga ggtcgtgagt tcgactctca
     1081 ttatccaccc caattcatat ggtctattag ctcagtcggt agagcacctg acttttaatc
     1141 agggtgtccc gcgttcgagt cgcggataga tcaccaaaaa ggtggcgaca tagccaagtg
     1201 gtaaggcagt ggactgcaac tccttgatcc ccagttcgaa tctgggtgtc gcctccaaaa
     1261 tattccaagg tagctcaacg gtggagcaac cggctgttaa ccggtaggct gtgggttcga
     1321 gccccaccct tggagc
//
LOCUS       DAELLV010000404         1272 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.407, whole genome shotgun sequence.
ACCESSION   DAELLV010000404 DAELLV010000000
VERSION     DAELLV010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.407"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..>1272)
                     /locus_tag="KPD58_003690"
     CDS             complement(<1..>1272)
                     /locus_tag="KPD58_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG0267380.1"
                     /translation="EYNLKLNKTLEGTKIIGKEVDIRKSIAKMMDDLLDENYEQHDIS
                     ELTRLDSATFSALINLFDIDSIIFVETIITELEYNLGYTINQPYYINLITHILICLKR
                     IEEGNQIESQEEREISVDNLDELVYKNVSTLIDKIENRYRVKITKDEIMYIYIFLVSS
                     GFSSENNSSYNDESDRLSESEKVSSIMIENMSRFLNINLKNDDLLQKSLASHVRPMLN
                     RLRYDIQIKNPLLGEIQERFSDVLGLCMMTINIMTEYDNLKNISIDEVSYLATYFQAA
                     IERNMSSKRVIVVCHTGYGTSQLLAARLKREFPEWTIVDIVPMHQLSKRNLDDVDFVI
                     STVNLDLKDKLHIVVSVLLMENDINNIKNALLSKPKKSMNLDTNLLGMYLEDNIYFNF
                     DVAQMESLIGVNLGSKYESISLGKDLNIYIH"
BASE COUNT          411 a          214 c          121 g          526 t
ORIGIN      
        1 tatgtatata tatatttaaa tcttttccta atgaaataga ttcatattta ctccctaaat
       61 tcacacctat taaactttcc atctgagcta catcaaaatt aaaatatata ttatcttcta
      121 aatacattcc taataagttt gtatctaagt tcatactttt tttaggttta gaaagtaaag
      181 cattttttat attattaata tcattttcca ttaatagtac tgatacaact atatgtaatt
      241 tatctttcaa atctaagttt acagtagata ttacaaagtc tacatcatct aaatttcgct
      301 tactcaactg atgcatagga actatatcaa ctatagtcca ttcaggaaat tctcttttta
      361 atctggctgc aaggagctga gatgttccat atcctgtatg acaaactact attactcttt
      421 tactactcat atttctttct attgccgctt gaaaatatgt ggcaagatac gaaacttcat
      481 ctatactaat attcttcaga ttatcatact ctgtcataat atttatagtc atcatacata
      541 atcccagcac atctgaaaat cgctcttgta tctctcctaa caacgggttt tttatttgga
      601 tatcatatct taatctgttt aacataggcc ttacatgaga tgctagactt ttctgtagta
      661 agtcatcatt tttgagattt atatttaaaa atcgtgacat attctctatc attatagatg
      721 aaactttttc actttcactt aatctatctg attcatcatt gtaactacta ttattttcgc
      781 tactaaatcc agatgatacc aaaaaaatat atatgtacat aatttcatct ttcgttattt
      841 ttactctgta tctattttct attttatcta taagtgtact gacattttta tatactaact
      901 cgtccaaatt atctacagaa atttctcttt cttcctgaga ttctatttga ttaccttctt
      961 cgattctctt taaacatata agtatatggg taattaaatt tatatagtag ggctgattta
     1021 tagtatatcc taaattgtat tctaattctg taattatagt ttctacaaat ataatagaat
     1081 caatatcaaa taaatttatc aatgcactaa atgtagcaga atctaatctt gttaactcag
     1141 atatatcatg ttgttcataa ttttcatcta ataaatcatc catcattttg gcaatcgatt
     1201 tccttatatc cacttcttta cctataatct tagttccttc taaagtttta ttaagtttta
     1261 aattgtattc tt
//
LOCUS       DAELLV010000405         1270 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.408, whole genome shotgun sequence.
ACCESSION   DAELLV010000405 DAELLV010000000
VERSION     DAELLV010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.408"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            126..575
                     /locus_tag="KPD58_003691"
     CDS             126..575
                     /locus_tag="KPD58_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0267381.1"
                     /translation="MEIREILTEESIILDLKSTTKEGIIQELVDKLYDTGSITLKSKF
                     IKALKHREKIGSTSIGYQMAMPHGKTKYVKKASIVIGKSKKGVDWPSIDGLPVKVIIM
                     FAMPQSGGNEDMILISKIAYILGGKNIGERLLNANTKKEILDIFYEV"
     gene            675..>1270
                     /locus_tag="KPD58_003692"
     CDS             675..>1270
                     /locus_tag="KPD58_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HBG0267382.1"
                     /translation="MAIYLEMPKLIHEFPFRSLSNEGEVLTTPHWHKEIEILLITEGV
                     VNLFINDKPMQLKKGEIVIIKGGDIHYILPSPGSERLVFQFDISFFNDLILLNEEKES
                     LINLFSSIKKCSRDWSAKIRKSVFDILIDIYNEDLYKIEGYSYAIKGKMYNLIPILYR
                     QIPREIEENTVEYEINSKEILERLNNIFKYVEKNYKQP"
BASE COUNT          570 a          113 c          208 g          379 t
ORIGIN      
        1 aaagaatatt ttaatggaaa tatcaaattt atataggcaa aagaactata taaactatat
       61 aatagattgc aagagcaaag aggatattaa aatattattt aagaataatc tattggagaa
      121 aacaaatgga aattagagaa atattaacag aagaaagcat tattttagat ttgaaatcaa
      181 ctacaaaaga aggcataata caggaattag tagataaatt atacgataca ggaagtataa
      241 ctttaaaaag taagtttata aaggcattaa aacatagaga aaaaataggc tctactagca
      301 taggttatca aatggccatg ccacatggaa aaacaaaata tgtaaagaag gcatccatag
      361 taataggtaa atctaaaaaa ggtgtagatt ggccaagtat agatggctta cctgtaaagg
      421 taataattat gtttgctatg ccacaatctg gaggcaatga agatatgata ttaatatcta
      481 aaatagctta cattttaggt ggtaaaaata taggtgaaag acttttaaat gcaaatacaa
      541 aaaaagaaat attagatata ttttatgaag tgtaggtatt gtataaatta taaatttgag
      601 gtttttaaaa taaaatgtgt attcttttaa atagtataaa ttaattttta gacactagag
      661 aggagaggat atatatggca atatatttag aaatgcctaa attaatacat gaatttcctt
      721 ttcgtagtct atcaaatgaa ggagaagtat taacaacacc acactggcat aaggaaatag
      781 aaatattact tataacagaa ggtgtagtaa atctatttat aaatgacaag cctatgcaat
      841 taaaaaaagg cgagatagtg ataataaaag gaggagatat tcattatata ctgccatcac
      901 cgggaagtga aagattagta tttcaatttg atatatcttt ttttaatgat ttaattttat
      961 taaatgagga aaaagaaagc ttaattaatt tattcagtag tataaaaaaa tgtagtagag
     1021 attggtcagc aaaaataaga aagtcagttt tcgatatatt gatagatatc tacaatgaag
     1081 acctatacaa aatagaagga tatagttatg caataaaagg taaaatgtat aatttaattc
     1141 ctatattata tagacagatt cctagagaaa ttgaagaaaa tacagtagaa tatgaaataa
     1201 actctaaaga gatattagag agattgaaca atatattcaa atatgtagaa aaaaattata
     1261 aacaacctat
//
LOCUS       DAELLV010000406         1265 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.409, whole genome shotgun sequence.
ACCESSION   DAELLV010000406 DAELLV010000000
VERSION     DAELLV010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.409"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            75..1250
                     /locus_tag="KPD58_003693"
     CDS             75..1250
                     /locus_tag="KPD58_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBG0267383.1"
                     /translation="MLGLYVHIPFCVKKCKYCDFNSYKMDIDSKKRYIEDLKIEMELY
                     SNKLYKDNKYKNKECCSLNKNDKITSIFVGGGTPSILTSDEIREVFISIKEMFDIDEN
                     AEITIECNPGTLTLEKLKTMKEIGINRLSIGLQAIQEKHLNFIGRIHTYEEFEKNYKD
                     ALSVGFKNINIDLMYSLPNQTLCDWKETLEKVVHLNPTHISAYSLILEEGTELYNMYE
                     SNKFELIDENVDIEMYEYTINYLKSKGYNQYEISNYSKEGYNCEHNILYWECEHYIGI
                     GAGASGYINENRYNNVESLEDYHLSLVKREKPIQENEILSEKDMIEEKIFMGLRMNKG
                     IKFEDFKKKFGIDFREKYNKQIEMLLARKLINQSFEGIQLTQKGREISNSVFIEFME"
BASE COUNT          556 a          102 c          220 g          387 t
ORIGIN      
        1 tgatgtgcta ttttgtttga tttataattt agtaattgat gttttaagat aatttaagaa
       61 atgaggagtg aaagatgcta ggactttatg tacatattcc attttgtgtg aaaaaatgta
      121 agtattgtga ttttaattct tataaaatgg acatagattc aaaaaaaaga tatatagaag
      181 acttaaaaat agaaatggaa ttatatagta ataaattata taaagataat aaatataaaa
      241 ataaagaatg ctgtagtcta aataaaaatg acaaaataac gagtatattt gtagggggag
      301 gtacacctag tatactaacc tctgatgaaa taagagaagt atttataagt ataaaagaaa
      361 tgtttgatat agatgagaat gcagaaataa ctatagagtg taatcctggg acattaacat
      421 tagaaaaatt aaaaactatg aaagagattg gcataaatag acttagtata gggctacaag
      481 ctattcaaga aaagcatttg aactttatag gaagaataca tacttatgaa gaatttgaga
      541 aaaactataa agatgcatta agtgtaggtt ttaaaaatat taatatagat ttaatgtaca
      601 gtcttccaaa tcaaactcta tgtgattgga aggaaactct tgaaaaagtt gtacacttaa
      661 atccaacaca tatatcagct tattccctta tactagaaga aggaactgaa ctatacaata
      721 tgtatgaaag taataaattt gaacttatag atgaaaatgt agatatagaa atgtatgagt
      781 atacaataaa ctacttaaag tcaaaaggat ataatcaata tgaaatatca aactactcaa
      841 aagaaggcta taattgtgag cataatattt tatattggga atgtgaacat tatataggga
      901 ttggagcagg agcttctggc tatattaatg agaacagata caataatgta gaatctttag
      961 aagattatca tttaagtttg gtaaaaagag aaaaaccaat acaagaaaat gaaattcttt
     1021 ctgagaaaga tatgatagaa gaaaagatat ttatggggct tagaatgaat aaaggcataa
     1081 aatttgaaga ttttaaaaaa aaatttggaa tagattttag agagaagtat aataagcaaa
     1141 tagaaatgct attagctaga aagcttataa atcagagctt tgaaggtata caattaactc
     1201 aaaaaggaag agaaatatct aatagtgtat ttatagaatt tatggaataa tttttaaggt
     1261 gttga
//
LOCUS       DAELLV010000407         1249 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.411, whole genome shotgun sequence.
ACCESSION   DAELLV010000407 DAELLV010000000
VERSION     DAELLV010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.411"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            122..622
                     /locus_tag="KPD58_003694"
     CDS             122..622
                     /locus_tag="KPD58_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBG0267384.1"
                     /translation="MRKNSINIALTAAITAIYAVLTLSLGFISYGPIQFRVAEIMMLL
                     AFLDKGYIVGLTLGCFLANVIGPYGVPDIIFGTLATFISASMIYITRKLGKQNKSTLV
                     IASLWPTVINSIVVGLMLNVFFGLPLILSMIQVGFGEFVVVTIVGVPFFCFLMKKYKN
                     VINIKF"
     gene            679..>1249
                     /locus_tag="KPD58_003695"
     CDS             679..>1249
                     /locus_tag="KPD58_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBG0267385.1"
                     /translation="MSKVKRLTDVWIYINDKQKFTIKELSEEFNLSAKTIQRYLKELN
                     KMGLPIQAEQGRNGGYRVLNNSYIPPVIFTEKEVMIILFALKSLQIYNYKLMEIEINS
                     IMRKVSYERKSSIKVGIENIKRYIGFVANDTNDGDFKSSNVTEFFRASSESLILNIKY
                     KCGNQILEKNVCPIGIYLNKGAWFSPAYDC"
BASE COUNT          472 a          111 c          218 g          448 t
ORIGIN      
        1 gaaaatagtt cgctaaaata atttgttttt ataaaattac tgtttaagtt tatagatttt
       61 gtataaaata aagttatgtt attaattttt ttaaaaataa aacttttact aggagacaga
      121 tatgagaaaa aatagtatta atatagcact aactgcagca attacagcaa tctatgcagt
      181 tttaacatta tcattgggat ttataagtta tggaccaata caatttaggg tagcagagat
      241 tatgatgctt ttggcatttt tagataaagg atatatagta ggtcttacat taggttgttt
      301 tttagcaaat gtaataggac catatggtgt acctgatata atttttggaa cattagccac
      361 atttataagt gcaagtatga tttatattac tagaaagtta ggtaaacaga ataaaagtac
      421 tttagttata gcttctttat ggccaactgt tataaattcg attgtggttg gtctaatgtt
      481 aaatgtattc tttgggcttc cattaattct atctatgata caggtaggtt ttggagaatt
      541 tgtagtagta acaattgtag gagtaccatt tttctgtttt ttaatgaaaa aatataaaaa
      601 tgtcattaat attaaatttt aaatagtgta tagtaattta attaattgag agtattatta
      661 gtgaaaagag gtgtttattt gtcgaaggta aaaagattga ctgatgtttg gatatatatt
      721 aatgataagc aaaagtttac aataaaagag ttgtcagagg aatttaatct ttcagccaaa
      781 acaatacaac gatatttaaa agaattaaat aaaatgggac ttccaattca agcagaacaa
      841 ggtagaaatg gaggttatag ggtgctaaat aatagctata tacctcctgt tatatttaca
      901 gaaaaagaag ttatgattat actatttgca cttaagtcac ttcagattta taattataag
      961 ttaatggaaa tagaaattaa ttctataatg agaaaagtaa gttatgaacg taaaagcagt
     1021 ataaaagtag gtatagaaaa tattaaaaga tacattggct ttgtagctaa tgatactaat
     1081 gatggagatt ttaagagtag taatgtaact gagtttttta gagcttcttc agaatctcta
     1141 attttaaata ttaagtataa gtgtggtaat cagattttgg aaaaaaacgt atgccctata
     1201 ggtatatatt taaataaggg agcatggttt tcaccagcat atgattgta
//
LOCUS       DAELLV010000408         1233 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.412, whole genome shotgun sequence.
ACCESSION   DAELLV010000408 DAELLV010000000
VERSION     DAELLV010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.412"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            105..>1233
                     /locus_tag="KPD58_003696"
     CDS             105..>1233
                     /locus_tag="KPD58_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBG0267386.1"
                     /translation="MNFNYNLPVNILFGRGRINDVGKEVSKYGKKVLIVTGKNSTKKT
                     GLLDKTIDLLKDSKIEYEVFDRVEQNPLITTIYSGVEIIKSTGCDCVLGLGGGSIMDA
                     AKSIAFSYKNPGDLNDYIFGIRQGEEALPIILVPTTSGTGSEGNCFAVLTNSETKDKK
                     SIKKNSMYAKASIIDPELMVTMPKHIIASVGFDALAHNMEAYLSNGRNPLADVQAIYG
                     IELISENLIKVYNDVNDLEAWEKITLASTIGGMTIGTAGTALPHGMEHPVSGLRDVMH
                     GKGLAALTPTIIESSWDSDIERFGNISKVLGGTCAEDCSDAIRNFLKKIDLDIKLSDL
                     GIEKSDIDWLTDNCMKISVANIKRHPKYFNKEQIKEMYHKSL"
BASE COUNT          486 a          134 c          232 g          381 t
ORIGIN      
        1 atataattaa gtaaaagatt gttttttagt ttatgtttaa atacataatt acactttgtt
       61 aaaaacataa aattattact aaagtagaaa taggaggttt tagcatgaat tttaattata
      121 atttgccagt aaatatctta tttgggagag gcagaattaa tgatgtggga aaagaagtat
      181 ctaaatatgg caaaaaagta ttgatagtaa caggtaaaaa tagtacaaag aaaactggat
      241 tacttgataa aacaatagat ttactaaaag actctaaaat agaatatgaa gtttttgata
      301 gagtagaaca aaatcctctt ataactacta tatattcagg agtagaaatt ataaaatcaa
      361 ctggatgtga ttgtgtttta ggtcttggtg gtggaagcat tatggatgct gccaagagta
      421 tagctttttc ttataaaaac ccaggagatt taaatgatta tatatttgga attagacaag
      481 gtgaagaagc tttacctata atcttagttc caacaacatc aggtacaggt agtgaaggaa
      541 attgttttgc agtacttaca aattcagaaa ctaaagataa aaaatctata aagaagaata
      601 gtatgtatgc aaaagcatca ataattgacc cagaacttat ggttacaatg ccaaaacata
      661 taattgcatc agttggattt gatgcacttg cccataatat ggaagcatat ctttctaatg
      721 gaagaaaccc tcttgcagat gtacaagcta tttatgggat tgaacttata tcagaaaacc
      781 taataaaggt atataatgat gttaatgatt tagaagcatg ggaaaaaata acacttgcaa
      841 gtactattgg aggaatgacg attggtacag cgggtacagc cttgccacat ggaatggagc
      901 atcctgtaag tggtcttaga gatgtaatgc atggaaaagg acttgcagct ttgactccta
      961 ctattataga aagttcatgg gatagtgata ttgaaagatt tggtaatatt tcaaaggtat
     1021 taggaggaac ttgtgcagaa gattgttcag atgcaattag aaatttcctt aaaaaaatag
     1081 atttagatat aaaactaagt gatttaggta ttgaaaaatc agatatagat tggttaactg
     1141 ataattgtat gaaaatatca gttgcaaata ttaagagaca tccaaagtat tttaataagg
     1201 aacaaataaa ggaaatgtac cataagtctt tat
//
LOCUS       DAELLV010000409         1233 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.413, whole genome shotgun sequence.
ACCESSION   DAELLV010000409 DAELLV010000000
VERSION     DAELLV010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.413"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..890
                     /locus_tag="KPD58_003697"
     CDS             <1..890
                     /locus_tag="KPD58_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBG0267387.1"
                     /translation="VSTISNNIILYTWIITVVVYLIYTIFKYIKLKNLINDLSYDVDD
                     REIVNLYKNILEEFNITKDIKLKYSYEVETPAFFNSCVLLPPHEYKLKELDWIFRHEL
                     MHFKSRDLYLKYVILFLKTVYWFNPFIYIMDKHIDLDCELYCDERVLKNRNSEEKKDY
                     ALTILNAMRKGSNTSNKFIAGLHKQSDIKKRVMNMFNEKYKNGILMALTLCLLSSITF
                     LKVNSTSIVNLNVLPKGFEDLRTVSSIRFSVIGFTYADAPKNMREEYKINCESLGLIP
                     KDSDKIEVGIEYYDSLLND"
BASE COUNT          507 a          107 c          173 g          446 t
ORIGIN      
        1 atgtttcaac tataagtaat aatattatat tgtacacttg gattataact gtagtagtgt
       61 atttaattta cactatattt aaatatataa aacttaaaaa tttaataaat gacttatctt
      121 atgatgttga tgatagagag attgtaaatc tttataaaaa tatattagaa gaatttaata
      181 taacaaaaga tataaaactc aaatactctt atgaagttga aacaccagca ttttttaatt
      241 catgtgtact tttgccacca catgagtata aattgaaaga gttagactgg atttttagac
      301 atgagcttat gcattttaag agtagggacc tttatttaaa atatgtaatc ctgtttttaa
      361 aaacagtata ttggtttaat ccatttatat atataatgga taaacatata gatttagatt
      421 gtgaacttta ttgtgatgaa agagttttaa aaaatcgaaa ttcagaggaa aaaaaggatt
      481 atgctttaac aattctaaat gcaatgagaa aaggttcaaa tacttcaaat aaatttatag
      541 ctggactcca taagcaatct gatattaaaa agagagttat gaatatgttt aatgaaaagt
      601 ataagaatgg tatattaatg gcattaactt tatgtttatt atcttctatt acttttttga
      661 aggttaattc aactagtatt gttaatctta atgtattacc taaaggattt gaagacttac
      721 gaactgttag cagtatacgt ttttcagtta taggatttac atatgcagat gctcctaaaa
      781 atatgaggga agaatataaa ataaattgtg aatcattagg tttgatacct aaagactctg
      841 ataaaataga agttggtata gaatattatg acagtctatt gaatgattaa ataaatatta
      901 ttttaacaga tattaattat aaagttttga atagtaattt gttgagaaat aaatatatcc
      961 ttataaaaat agatacttat ataccaagaa taaaaaatag tcaagatagt acacaaggat
     1021 atacttataa agaacttaaa taagtgaaat aaattattaa ttataaggtt tataatttta
     1081 aaaaattaga taaattcagg tgtaaaatac tataaaacac aattcggaaa agtgtatgtt
     1141 gaacttcatt taaaatggaa tttcaaagtt gctatagtaa gtagtttaag cattcttctt
     1201 atattaatct ttgagaataa tgtatttaaa aaa
//
LOCUS       DAELLV010000410         1228 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.414, whole genome shotgun sequence.
ACCESSION   DAELLV010000410 DAELLV010000000
VERSION     DAELLV010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.414"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(388..1119)
                     /locus_tag="KPD58_003698"
     CDS             complement(388..1119)
                     /locus_tag="KPD58_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0267388.1"
                     /translation="MIRKKVYILISIAICDDESYMLNHLKENIELYMKQKNLLYNIEL
                     FNKVEDLLCNHEKFNIVFLDIQMEEINGMQAARKLRSYGNECFIIFVTVLNEFVFDAF
                     EVDAINYLIKPLSNEKLYKTLDKIVTKLDDNESNYITIQKGYSYWKLKLADILYCEVI
                     KRKIYVHLKKETVDYYETIENLERSLPTNFFRCHRSYIINLQYVYGYENGNAVMESGD
                     RIPVSRLRQQIFSKVMLEFMKNRRI"
BASE COUNT          481 a          170 c          105 g          472 t
ORIGIN      
        1 atatctgtat ttatatcact agtaaatatc aagtaattta atggagctgt aaatccatat
       61 actaattgtt caattaaata tactaacaaa gttgatacta tagataaaac agtagattgt
      121 tttattatgt actttacaaa taaaaaagtc ataagaaaaa atagaatctg tgaagaaaat
      181 aaattatata aattaaaata tgtatttata atgattaaaa aaattgaaat tatcgaatat
      241 aaaataaaac accaaaattt tgaattaaca tttgtaaaac tgattagaaa atagtattgc
      301 actattacca ttaaaattct atatacaata aaagaaaata aaaaactgta ctttaaaaat
      361 aaatcggaag aaaataaatt aatcatctta tatcctccta tttttcatga actcaagcat
      421 aacttttgaa aatatttgtt gacgtagtct agaaacaggt attctatctc cactttccat
      481 aactgcatta ccattttcat atccatatac atattgtaaa ttgattatat aactacgatg
      541 acacctaaaa aaatttgttg gtaaacttct ctctaaattt tcaatagtct cataataatc
      601 tacagtctct ttctttaaat gaacataaat ctttcttttt ataacttcac aatataaaat
      661 atcagcaagt tttaatttcc aatatgaata acctttttgt attgtgatat aattactttc
      721 attatcatca agttttgtaa ctattttatc caaagtttta tatagtttct cattagatag
      781 aggttttatc agataattta tagcatctac ttcaaaagca tcaaatacaa attcatttaa
      841 aactgtaacg aaaattataa agcactcatt accatatgac cttaattttc tagctgcctg
      901 cattccgtta atctcttcca tctgtatatc taaaaataca atattaaact tttcatgatt
      961 gcatagtaaa tcttctactt tattaaaaag ctctatatta tataataaat tcttctgctt
     1021 catgtataat tctatatttt cctttaaatg atttagcata taggattcat catcacaaat
     1081 ggcaattgaa atcaatatat acaccttctt tcttatcatt attctaatta ttatataaag
     1141 ttaatttttt ctgttcaatt aatatgatct aatatgttga atatttgttc caaaatgtaa
     1201 taatctcgct tttcatagta tatctaat
//
LOCUS       DAELLV010000411         1195 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.415, whole genome shotgun sequence.
ACCESSION   DAELLV010000411 DAELLV010000000
VERSION     DAELLV010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.415"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..>1195
                     /locus_tag="KPD58_003699"
     CDS             <1..>1195
                     /locus_tag="KPD58_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0267389.1"
                     /translation="SLGTIIISVSKPLMLSVKTDPMICIRRNNEKVDIKQNSFINKFM
                     IKLFKDYGNIASKNIQRNKKRTNLSIASMVIIFFLMATIYTKSTSNFLSDGVLKYWIQ
                     GDYLLHNIDISTIRSNNKGYDKNVLQEIKAIDGVTKVNSYRHKWFNIKIDDKSINKNS
                     DYWKKNKDNIEARSEVKDGIKVYNNSFEFLGIEDTDILEDFLINGKENIDKFSKQPYL
                     YITKKSSDSLNIKKGDRIKVYFDIIDSKTNNFTKTISQEFIVCGIITTLPVTSQVGAE
                     FGGVISTNQFNKFTGISSYERFDIWTSKLANDKYVESELNKITEKTNKGILIPYKAET
                     AEYEKIDNQKTLIMVLVTGIIVILSLFNCCNTIVTSINSRSREFALFRGIGISKHEIN
                     KIVKLE"
BASE COUNT          542 a          115 c          176 g          362 t
ORIGIN      
        1 aagtttagga actataataa tatcagtttc aaaacctctt atgttatcag ttaaaactga
       61 tccaatgatt tgtataagaa gaaataatga aaaagtggat ataaaacaaa attcctttat
      121 aaataaattt atgataaaac ttttcaaaga ctatggtaac atagcatcaa aaaatataca
      181 aagaaataag aaacgtacaa atttatctat agcatcaatg gtaattatat ttttcttaat
      241 ggcaacaata tacacaaagt ctactagtaa ctttttaagt gatggtgtat taaaatattg
      301 gatacaagga gattatttat tacataatat tgatataagt actatacgtt caaataataa
      361 aggttatgat aaaaatgtac tacaagagat aaaggcaatt gatggtgtaa caaaagtaaa
      421 ctcatatagg cataagtggt ttaatataaa aattgatgat aaaagtataa ataagaactc
      481 tgattattgg aaaaaaaata aagataatat tgaagctaga tcagaagtaa aagatggaat
      541 caaggtatat aataattctt ttgaattttt aggaatagaa gatacagata tactagagga
      601 tttcttgata aatggaaagg aaaatataga taaatttagt aaacaaccat atctgtatat
      661 aacaaagaaa tccagtgatt cgcttaatat aaaaaaagga gatagaataa aagtatactt
      721 tgatattata gattcaaaaa caaataattt taccaaaaca atatctcaag aatttatagt
      781 atgtggaatt ataactacat taccagtaac ctcacaagtg ggagctgaat ttggcggagt
      841 aatatcaaca aatcaattta ataagtttac aggaatatca tcttatgaaa gatttgacat
      901 atggacgagt aaactagcca atgataaata tgttgaaagt gagttaaaca aaataacaga
      961 aaaaacaaat aaaggaattt taattccata caaagctgaa acagctgaat atgaaaaaat
     1021 tgataatcaa aaaacactaa ttatggtatt agtaactgga attatagtta tattatcttt
     1081 atttaactgt tgcaatacta tagttactag tattaatagc agaagtaggg aatttgcact
     1141 atttagagga ataggaatat caaaacatga aataaataaa attgttaaat tggaa
//
LOCUS       DAELLV010000412         1172 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.416, whole genome shotgun sequence.
ACCESSION   DAELLV010000412 DAELLV010000000
VERSION     DAELLV010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.416"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(307..798)
                     /locus_tag="KPD58_003700"
     CDS             complement(307..798)
                     /locus_tag="KPD58_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HBG0267390.1"
                     /translation="MGMIGCYTKISEENVFKLQQAEENLQDFVFEDANENSTINIDKA
                     WHAIHFTLTGCPFGGDDDNIFSKLVLSGNILMEIDGEFPVMLITANDVKKLSKAMNSL
                     EEQAFRKRFNINEMLENNIYPVMNDESEDDFFEYVWANLIELKKFIQEASNDGLAVIF
                     FIM"
BASE COUNT          453 a          168 c          107 g          444 t
ORIGIN      
        1 gtctattttt atatataatg aaaaatgtga taagtatcca aaagaattac tggatataat
       61 aagatagggt atttagcctt ttaagataaa taaaaatatc tgcaactata tattgcaaaa
      121 atttaaaact aatttatata tgctaatata aatatttata aagacataag acaaatttac
      181 ctacagattt aaatttaact tatatatttt aaaaggctaa tataatatgt attttcttct
      241 atacaatcaa aaggcttcat atctaagata tgtgatgaca ctaatattca caatttttag
      301 taattatcac attataaaaa atattacagc tagtccatca ttagatgctt cttgtataaa
      361 ctttttaagc tcaattagat ttgcccatac atattcaaaa aaatcatctt cactctcatc
      421 attcataaca ggataaatat tattttctaa catctcatta atattaaatc tttttctaaa
      481 tgcttgttct tccaaactgt tcattgcttt tgatagtttt tttacatcat ttgctgtaat
      541 caacatgaca ggaaattctc catcaatttc cataagtatg tttccactta gtactaattt
      601 gctaaatata ttatcatcat ctccaccaaa aggacaacct gtcaaagtaa agtgaatggc
      661 atgccatgct ttatcaatat tgatagtact attttcgttg gcatcttcaa aaacaaaatc
      721 ttgtaaattt tcttctgctt gctgtaattt aaaaacattc tcttcactta ttttagtata
      781 acatcctatc atccccataa ttacattcct cctaagtagc gtttttttaa ttgtaactta
      841 taattaaact tagttacaat aaaatatatt gagaaaagta ttatttcatc ttaaatttaa
      901 atacctatat tatcaactaa aattctgtgt ttatagcgta agtattatta ttttttccta
      961 aaaatcaaat gaatcagaag ttctataggc aatattgact ataaataaga actattactt
     1021 ttaaaaaaaa tttaagaaat tatactgtag aatcatgctc tacttattag cttcaaataa
     1081 aaaataaatc atgttctcaa attaaataat aaataacaat aaatttaaat tttaaactat
     1141 aagagtttct gaagtgttaa taagcatacc aa
//
LOCUS       DAELLV010000413         1153 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.417, whole genome shotgun sequence.
ACCESSION   DAELLV010000413 DAELLV010000000
VERSION     DAELLV010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.417"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(292..1038)
                     /gene="cdtR"
                     /locus_tag="KPD58_003701"
     CDS             complement(292..1038)
                     /gene="cdtR"
                     /locus_tag="KPD58_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family response regulator CdtR"
                     /protein_id="HBG0267391.1"
                     /translation="MDILIFDNDVCFGMKLKEKINNILIKEGFDNDVVRLYHNANLLL
                     KELTEKNKVRIYFIVVDAKYKISNELCDGLWIAQKIRESDYISPIIFLTNHIEMILGI
                     FDYRLEVMDFILKHDMEIAESKIKACIKIAHKRYIKEKNYRSNFFTIYSDFSLWKISF
                     DEVIYFETSTIPHKIKLVTTSRIFEFYKSLKSLPDLDACFIRVHKSFVVNKYHIVSLD
                     LKKNNIKMSNGHICRISNTYRNILKNIIKT"
BASE COUNT          431 a          139 c          121 g          462 t
ORIGIN      
        1 atatgtaggt atatatcata aaaatatgta tttatatgat atgtcagtac aaaataacaa
       61 ctgaatattt taaagagact atttcgtact tttatgattt aatgagctat cttatcgaat
      121 cataatctag gaacaattgt tataatatct aatagtagtt atatatgata tagtcagtat
      181 gtgttttttc acaatatgat tattatatag aggattctta gggattactc tttttgtata
      241 aaaaagagat ggttcaaatt tttatcttga gacatctcct ttttctattt attatgtttt
      301 aataatattc tttaaaatat ttctatatgt attagatata cgacatatat ggccattact
      361 cattttaata ttatttttct ttaggtcgag agaaacaata tgatatttat taactacaaa
      421 agatttatgt acacggataa agcatgcatc taaatctggt aaagacttta aacttttata
      481 gaattcaaat attctagaag tagtcactaa ttttatttta tgtggaatag tgcttgtttc
      541 aaaatatata acttcatcaa atgaaatttt ccatagtgag aaatctgaat agatagtaaa
      601 aaaattagag cgataatttt tttctttaat gtatcttttg tgagcaattt taatacaagc
      661 ttttatttta ctttcagcaa tttccatatc atgttttagt ataaagtcca taacttctaa
      721 cctataatcg aagatcccta aaatcatttc tatatgattt gtaagaaata tgattggact
      781 aatataatct gattctctaa ttttttgtgc tatccataat ccatcacata attcattact
      841 tatcttatat tttgcgtcta caactataaa atatattctt actttatttt tttcagtaag
      901 ctcttttaat aacaaatttg cattatgata taatcttaca acatcattat caaaaccttc
      961 ttttattaaa atattattta ttttttcttt tagtttcatt ccaaaacaaa catcattatc
     1021 aaagattaat atatccacta aataccctcc tataaaaaat tcaaaagtaa aaaaacaaaa
     1081 gtattttata gaaaatacat aaaataacac taggagataa ataagattaa ggatagttag
     1141 aattaaggta tat
//
LOCUS       DAELLV010000414         1130 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.418, whole genome shotgun sequence.
ACCESSION   DAELLV010000414 DAELLV010000000
VERSION     DAELLV010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.418"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            394..1044
                     /locus_tag="KPD58_003702"
     CDS             394..1044
                     /locus_tag="KPD58_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0267392.1"
                     /translation="MKVKSNLDEILYHKIIESLIRGEYSVGQKILLNDLCEKFEVSRT
                     PVVQAVKMLNKDGVLTIMTNGKVYVPEYEYDMVKQVCETRTLIETYALEKMMQEEEEV
                     FQQKLDVIKRYSDKCEEYYRQEKSVELALADLELHKAIVEGANNEILNDVYVGIQGRF
                     IVVNYLIRPLKNRNYEGTVQDHFEILKFIEDRDTKKAVDKLRNHIQGTIKRFCEDE"
BASE COUNT          481 a          108 c          185 g          356 t
ORIGIN      
        1 tagtacaata acttgatttg caatacaatg aattaatcta tggtataata gtttaatttg
       61 aagtacaatg aattaatcta tagtataata gtttaatttg aagtacaacg atttaatcta
      121 tagtataata gtttaatttg aagtacaatg gtttaatcta tagtataata gtttaatttg
      181 aagtacaacg atttaatcta tagtacaata acttgatttg caatacaatg aattaatcta
      241 tagtataaga gcatgattta cagtacaata atttaaattg tagtacaaaa gtactatcaa
      301 ataagaattt ctactttaaa aatattacta aaagtaatta aagtccagta attcaatata
      361 atatggcaaa agaatagaaa taaggtgaga gtagtgaaag taaagtcaaa tttagatgaa
      421 attttatatc ataagattat tgaaagtctt attcgtggtg aatactctgt aggtcaaaaa
      481 atattactaa atgacctttg tgaaaaattt gaagtaagca gaacaccagt tgtacaagca
      541 gtaaaaatgc taaataaaga tggagtgctt actattatga caaatggtaa agtgtacgta
      601 ccagagtatg aatatgatat ggtaaaacaa gtttgtgaaa cgagaacact gattgaaaca
      661 tatgctttag aaaaaatgat gcaagaagag gaagaggttt ttcaacaaaa gctagatgta
      721 ataaaaagat actctgataa atgcgaggag tactatcgtc aagaaaaatc tgtagaactt
      781 gcactagcag atttggaatt acataaagcc attgtagaag gggcaaataa tgagatttta
      841 aatgatgtat atgttggtat acaaggaaga tttatagtag tgaactattt aattcgacca
      901 ttaaaaaatc gtaattatga aggaacagta caagaccatt ttgaaatatt aaaattcatt
      961 gaagacagag atacaaaaaa agcagtagat aaactaagaa atcatattca aggaactatt
     1021 aaaagattct gtgaagatga ataataagtt ttgaagttac tatatatact tatctattaa
     1081 gttgctaaat gtagtctaag tataatctaa ttatagttta aacataatct
//
LOCUS       DAELLV010000415         1118 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.419, whole genome shotgun sequence.
ACCESSION   DAELLV010000415 DAELLV010000000
VERSION     DAELLV010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.419"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            31..921
                     /locus_tag="KPD58_003703"
     CDS             31..921
                     /locus_tag="KPD58_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0267393.1"
                     /translation="MAKLTLEHIKGNTYYIPLPTIVGVYVDGKDAILIDSGNNKDTAR
                     QVLRLLEEHNLNPKLIINTHSNADHIGGNAYLKNQTKCKIATTKIEGYFTENPILESA
                     FLYGGYPSKALKNKFLLAKESEVDYIIPSNGKIVDTELEAISLPGHYFEMIGVRTPDN
                     VLFVADSLTPENIITKYHFFFLLDIDSQFKTLEKLRMLEADFFVPSHSVKTTDIKSLI
                     NINKKKMEEIIDNIKKVCYEPVMIDEVIEKMCDLYNVKLDANQYVLVGSTIRSYITYL
                     YENNMVEYIFDGGKMMIKAA"
BASE COUNT          448 a          107 c          187 g          376 t
ORIGIN      
        1 ttaaaagtaa atttaaaggg aggttataaa atggctaaat taactctaga acatataaaa
       61 ggaaatacat attatattcc actccctaca atagtaggtg tatacgtaga tggtaaggat
      121 gcaattttaa ttgacagtgg aaataataag gatacagcta gacaagtttt aagattatta
      181 gaagaacata atttaaatcc taaattaata ataaacactc attcaaatgc agaccatata
      241 ggaggaaatg catatttgaa gaatcaaaca aaatgcaaga ttgctacgac taaaattgaa
      301 ggatatttta cagaaaatcc aatattagaa tcagcatttt tatatggtgg ctatccaagt
      361 aaagcactga agaataaatt tttattagca aaggaaagtg aagttgatta tattatacct
      421 tcaaatggca aaattgtaga tacagaactt gaagcgattt ctcttccagg tcattatttt
      481 gaaatgattg gtgttagaac tccagataat gttttgtttg ttgcagatag tcttacacca
      541 gagaatataa ttactaaata tcattttttc ttcctactgg atatagattc acagtttaag
      601 acactagaaa aattgagaat gttagaggct gatttctttg ttccaagtca ctctgtcaaa
      661 actacagata taaaaagttt aattaatatt aataagaaga agatggaaga aataatagat
      721 aatattaaaa aggtttgtta tgagccagtt atgattgatg aagttattga aaaaatgtgt
      781 gatttgtaca atgtcaagct tgatgctaat caatatgtgt tggttggaag tacaatacgt
      841 tcttatataa cttatttata tgagaataat atggttgaat atatatttga tggtggtaag
      901 atgatgataa aagctgcgta aatagtaata attttcaaat tatatgatga aattgtttta
      961 gtatgactgt tatttgaaaa gtaatataaa aaaatttata taaataagct aagatgattg
     1021 atataaagtt actgataatt ttaaggtaat atatacgtta ctataatatt atgttattat
     1081 ttgtaaataa ggggataatt tataagcaaa ttaaagac
//
LOCUS       DAELLV010000416         1118 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.420, whole genome shotgun sequence.
ACCESSION   DAELLV010000416 DAELLV010000000
VERSION     DAELLV010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.420"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(210..920)
                     /locus_tag="KPD58_003704"
     CDS             complement(210..920)
                     /locus_tag="KPD58_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0267394.1"
                     /translation="MTPICICDDEKQQRNMLNKIITREMDLWGYPYQIKEYSNGESLV
                     YNLEHENYYIDIIFLDIELGKSNGVDIAKTIRKHSKDTIIIFVTGFSDYVFHGYDVGA
                     LNYILKPYKTEKICKVLKEALKKLEQWKENFITVQIGTNLCKINTKDILYFKSELRKL
                     TVVTFDKTLEYYGKLNDMETVLPSNFIRTHQRYIVNLNHVDSITQTYAKVQNEKIPIS
                     RKKYQEVVGKFTRYMLES"
BASE COUNT          400 a          149 c          124 g          445 t
ORIGIN      
        1 ttgaaagatt gaaattctag aaactgagct ttaaaaaatt aagtttgaaa aaaactctaa
       61 aaactaagct tcaaaaaatt aaattttgaa agattgaaat tctagaaatt aatcttcaaa
      121 aaattaaatt tgaaaaaaca aaactctaaa aactaagctt taaaaaatta agtttcaaaa
      181 ataaaattct agagattgaa cttcaaaaat taagattcta acatatacct tgtaaatttt
      241 cctacaacct cttgatattt tttacggctt ataggaattt tttcattctg aactttggca
      301 taagtttgag ttatactatc tacatggttt agattgacaa tatacctttg atgcgtacgt
      361 ataaaattag agggaagaac agtttccata tcatttaatt ttccgtagta ttctaatgtt
      421 ttatcaaagg taactacagt taattttctt agctcacttt taaagtagag gatatctttt
      481 gtattaattt tacaaagatt agttccaatc tgaactgtaa taaagttttc tttccattgt
      541 tctaattttt ttagtgcttc ttttaatact ttacatattt tttcagtttt ataaggtttt
      601 aagatgtagt tcaatgctcc tacatcatat ccatgaaata catagtcaga aaaacctgtg
      661 acaaagatga ttatagtatc ctttgaatgt tttcgtatag tttttgctat gtcaacgcca
      721 ttactttttc caagttcaat atctaaaaaa ataatatcta tgtaataatt ttcgtgttct
      781 agattgtata ctagactttc tccatttgaa tattctttta tttggtaggg gtagccccac
      841 aaatccattt ctcttgtaat gattttattc agcatgtttc tttgttgttt ttcatcatca
      901 caaatacaaa tcggagtcaa ttttttatct cctttcaaat tttatattta tatagttaag
      961 tatataatat attttgctac ctagatatat aaaatgaaat aacaattata tccagatagc
     1021 aaaataaaat tttttatatt agtatactta cacttaaaac tattctatta aacttattct
     1081 gaagtgctaa tgattttctt attaaaaaat ctaacaat
//
LOCUS       DAELLV010000417         1114 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.421, whole genome shotgun sequence.
ACCESSION   DAELLV010000417 DAELLV010000000
VERSION     DAELLV010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.421"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            121..1005
                     /locus_tag="KPD58_003705"
     CDS             121..1005
                     /locus_tag="KPD58_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HBG0267395.1"
                     /translation="MDTTKIDDISKLDLNNLDINKMDMGGIMESLLEWATTSGVKLII
                     GILILSIGFKIIKKFVNHVMLVLSKRDIDLTLRRFLKSLLLSVLKVAVIIIVLEYWGM
                     SLSSFAAVIASAGVAIGLALQGSLSNFAGGFIILLIRPFKVGDYVEAAGHGGTVEQIG
                     LFYTQLVTPDNKQILIPNGSVSNDSLINYSAKNTRRVDLTFSVGYEDDILHVRRVLKD
                     IVNNHKLIINEPEPFIGVVEHGDNAIKFATRVWCKTEDYWTIYLDLLEEVKVRFDEEG
                     ITIPYPKMDLTVKELNKI"
BASE COUNT          422 a          103 c          209 g          380 t
ORIGIN      
        1 atgtataatt ggaatataaa tcttgtttac tatgtacaaa tggtattgta ttatattaaa
       61 tagaaaataa gaactttaaa ctataatatt ggttctaaaa tataaggtaa ggagtttttt
      121 atggatacga cgaaaataga tgatataagc aagttagatt taaataattt agacattaat
      181 aagatggata tgggaggtat aatggaaagt ctgctagaat gggctactac aagcggagta
      241 aaacttataa taggtatact tatactttct attggattta aaataataaa aaaatttgta
      301 aaccatgtta tgttagtact tagtaaaaga gatattgatt taactttaag aaggtttttg
      361 aagtctcttc ttttgagtgt acttaaagtt gccgttataa tcattgttct ggaatattgg
      421 ggaatgagtt tgtccagttt tgctgctgtg atagcatcag caggggtagc aataggtttg
      481 gctttacaag gtagtctatc aaattttgct ggaggattta ttatactatt gattagacct
      541 tttaaagtag gagattacgt agaagcagca ggccatggag gaacggttga acaaatagga
      601 cttttttata cacaattggt tactccagat aataaacaga tacttatacc taatggttct
      661 gtatcaaatg atagtctaat aaactattca gcaaagaata ctagacgagt tgatttgact
      721 tttagtgtag ggtatgaaga tgatatacta catgttagaa gagtacttaa agatattgtt
      781 aataatcata aattaataat aaatgaacca gaaccattta taggagtagt agaacatgga
      841 gataatgcta ttaaatttgc aactagagtt tggtgcaaga cagaagatta ttggacaatt
      901 tatcttgatt tactagaaga ggttaaagtt aggtttgacg aagaaggaat tacgattcca
      961 tatcctaaaa tggatttaac agtaaaagaa ttaaacaaaa tttaattttt taatatagtg
     1021 tttaaattta agaatatcat atagatatac ttataaggtt tgtaaaaaat taataagtac
     1081 tgttattttg taagtaaaaa tataaaaaat gggc
//
LOCUS       DAELLV010000418         1102 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.422, whole genome shotgun sequence.
ACCESSION   DAELLV010000418 DAELLV010000000
VERSION     DAELLV010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.422"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..380
                     /locus_tag="KPD58_003706"
     CDS             <1..380
                     /locus_tag="KPD58_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBG0267396.1"
                     /translation="MIPMFLYDFSSDKVIALIARILYFSDYFIVILFLIFIINSTKYS
                     PFVLENVKRFKVMGCCLLINTIIECINGYNLNTNNIRIFGTDSGGISPLMVISFIFAL
                     MCFVIAETFDKAIKIKEDNDLTI"
     gene            391..606
                     /locus_tag="KPD58_003707"
     CDS             391..606
                     /locus_tag="KPD58_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0267397.1"
                     /translation="MAIVVNLDVMMAKRKISLKDLAEKIDITNANLSILKTGKAKAIR
                     FTTLNEICKALDCQPGDILEYVEDEND"
BASE COUNT          403 a          132 c          144 g          423 t
ORIGIN      
        1 gtatgatacc tatgttttta tatgatttta gttctgacaa agtaatcgct ttaattgcaa
       61 gaatactata tttttctgac tactttatag tcatcttatt cttgatattt ataattaata
      121 gtactaagta ttccccattc gtacttgaaa atgtaaaaag gtttaaggta atgggatgtt
      181 gtttattgat aaacactata attgaatgta taaatggtta taatttaaat actaataata
      241 taagaatttt tggaactgat agtggcggta tctcaccact tatggttatc tcttttatat
      301 ttgcactaat gtgctttgta attgcagaaa catttgataa agctataaaa ataaaagaag
      361 ataatgattt aacaatatag aggtgatgtt atggcaatag tagttaacct agatgtaatg
      421 atggcaaaaa ggaaaatatc acttaaagat ttggcagaaa aaatagatat tacaaatgca
      481 aatttatcaa ttttaaaaac tggaaaagca aaagctatta gatttaccac tctaaacgaa
      541 atttgcaaag ctttagactg tcagccaggt gatatacttg aatatgtaga agatgaaaat
      601 gattaaaaaa gagtgtttag aaacactctt ttttattata ctatatgatt tttattcttt
      661 tactatatta ttgacattaa tattaacttt gtttaaagtc ataacaaaat atgttctaag
      721 aaagaaactg ttcctttatc tatttgttat ccattaccta taagaactgt atcacactaa
      781 atcatacttt taaattgata attttaccac agattcctat atacattctt ttctacctaa
      841 tatttgttta aactgttagc aaaacatgga actttttcat ttacttatac ctccatttat
      901 attattgcat agtttatagc tacgtgttat aaattacatt attgatgatt ttatctaaca
      961 tttgtaatct tttagatact ctttgaagtt acaacactgt tatactctaa ttaagtaaac
     1021 taaatctgat acaagttggt gattatatga aaagaaaact ttcaacctat tttaaaggat
     1081 atattttagg tttatgtata gc
//
LOCUS       DAELLV010000419         1097 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.423, whole genome shotgun sequence.
ACCESSION   DAELLV010000419 DAELLV010000000
VERSION     DAELLV010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.423"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            163..915
                     /gene="cwlD"
                     /locus_tag="KPD58_003708"
     CDS             163..915
                     /gene="cwlD"
                     /locus_tag="KPD58_003708"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase CwlD"
                     /protein_id="HBG0267398.1"
                     /translation="MEYYSVFISIHIQGDVVRKYIKHIIFSFAMICLVVVSIFEIKNI
                     SEDVIKYMPVTNKTIILDAGHGGIDPGALNKDKSTSEKDINLAITLKLRELIESSGGL
                     VILTREDDSSLYKEENNKTTRQKYNENLKNRKEIISNSNANMFVSIHLNAFEQSKYYG
                     AQTFYPKDKQDSKELSKCIQEELKRVVDKTNNREVKPRDDIYLLKENNIPSVLIECGF
                     LSNEKECKLLTDETYQEKIAWAIYIGIQKYLS"
BASE COUNT          487 a          111 c          166 g          333 t
ORIGIN      
        1 tataatataa actaagtata atttattgta tagtattgaa tgttagataa acatagtgaa
       61 ttaaataggg gcagaaatat aatatcatta cataataata tattaatacc aataaataaa
      121 gatatatcaa tattaaaaaa atttaaatgt ttgtactaat atatggaata ttatagtgtt
      181 tttataagta tacatataca gggtgatgtt gtgagaaagt acataaaaca tataattttt
      241 agctttgcaa tgatatgtct agtagtagta tccatatttg aaattaaaaa tatttctgaa
      301 gatgttatca agtatatgcc agtaacaaac aaaactataa ttttagatgc tggtcatgga
      361 ggcattgatc caggtgcatt aaataaggat aagagtacat ctgaaaagga tattaaccta
      421 gcaataacac ttaagcttag agagcttata gaatcaagtg gtggccttgt aatattaact
      481 cgagaagatg acagtagcct ttataaagaa gaaaataata aaactacaag acaaaaatat
      541 aatgagaatt tgaaaaatag aaaagaaata atatctaatt ctaatgccaa tatgtttgtc
      601 tctatacatc ttaatgcatt tgaacaatca aaatactatg gagctcaaac tttttatcct
      661 aaagacaaac aagacagtaa agaattatcg aagtgtattc aagaagaact taaaagagta
      721 gtagataaga caaacaacag agaagtcaaa ccaagggatg atatatatct tttgaaagaa
      781 aataatattc catcagtact gatagaatgt ggttttttat caaatgaaaa agagtgtaaa
      841 ctcttaactg atgaaacata tcaagaaaaa atagcatggg caatctacat aggaatacaa
      901 aaatatttaa gttgaaataa ctataaaaat aggtctaaat atgtaaatag cttgtattaa
      961 agcagttaaa atgcattaat ataggctatt tttattttga caaaaaaata tttaaaataa
     1021 aagttaaaaa gttgttgact tagaataata tagatgatat tatatatgag tgcccaaaag
     1081 gagcaccaaa ataagac
//
LOCUS       DAELLV010000420         1091 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.424, whole genome shotgun sequence.
ACCESSION   DAELLV010000420 DAELLV010000000
VERSION     DAELLV010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.424"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            33..1064
                     /locus_tag="KPD58_003709"
     CDS             33..1064
                     /locus_tag="KPD58_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HBG0267399.1"
                     /translation="MLKKKSSLTLKTIREILPGLFVSVLVGYISIFISILIPKVGAAS
                     ISIFLGMFVGNLFLNQKVFQKGYKFSETDLLSYSIVLLGATLSVSTLIDLKVSGILFI
                     ILQMTITIIAALYIGKKLGFEENFRFLMASGNAVCGSSAIGATVPVVNATDKEKGIAV
                     TIVNVTGIFLMFLLPIISQFLYSHELVKTSAMIGGTLQSIGQVVASGAIVGENVKDLA
                     TIFKIVRVIFLVVVVLVFGHMKNQSNREILEEEKDEIKKKKVTIPWYVIGFFITCALF
                     SMNIIPQEVSVLCKEISNKLEIIALAAIGLKVNVKDLVKQGKEVSLYGLFVGTVQVVS
                     AVVLIKIFI"
BASE COUNT          411 a          107 c          196 g          377 t
ORIGIN      
        1 gaaaactata ttaattataa ggtaggtata ttatgttaaa gaaaaaaagc agtttgacat
       61 taaaaactat aagggaaata ttaccagggc tgtttgtatc agtattagta ggttatatta
      121 gtatatttat ttctatttta attcctaagg taggggcagc atcaatttct atatttttag
      181 gtatgtttgt aggaaaccta tttttaaatc aaaaggtatt tcaaaaaggt tataaatttt
      241 cagagacaga tttgttatca tattcaatag tactattagg agcaacactc agtgtatcta
      301 ccttaataga cttaaaagtt tcaggaattt tatttataat attgcaaatg acaataacaa
      361 taatagctgc actttatata ggtaaaaaac taggatttga agaaaacttt agatttctaa
      421 tggcaagtgg aaatgcagta tgtggttctt ctgcaatagg agcaacagtt ccagtggtta
      481 atgcgacaga taaagaaaag ggaattgcag taactattgt aaatgttact ggtatatttc
      541 ttatgttttt acttccaata atatctcaat ttttatatag ccatgaactt gtaaaaactt
      601 cagcaatgat aggtggaact ttgcaatcaa ttggtcaggt tgtagcaagt ggagcaattg
      661 ttggagaaaa tgtaaaagat ttagctacta tttttaagat agtaagagta atatttctag
      721 ttgttgttgt attagtattt ggacatatga aaaatcaatc taatagggaa attcttgaag
      781 aagaaaagga tgagataaaa aagaaaaaag tgactatacc atggtatgta attggattct
      841 ttataacttg tgcacttttt tcaatgaaca ttataccaca agaagtatct gtgttgtgca
      901 aagaaataag taacaaacta gaaataatag ctttagcagc aattggatta aaggttaatg
      961 taaaagattt agtgaaacaa gggaaagaag tttctttata tggtttattt gtaggtacag
     1021 tacaagttgt atcagctgta gttttaataa aaatatttat atagcataaa aatagtattt
     1081 gtaatattaa t
//
LOCUS       DAELLV010000421         1076 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.425, whole genome shotgun sequence.
ACCESSION   DAELLV010000421 DAELLV010000000
VERSION     DAELLV010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.425"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            411..878
                     /locus_tag="KPD58_003710"
     CDS             411..878
                     /locus_tag="KPD58_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HBG0267400.1"
                     /translation="MKFLNVLVVVEDIEKSKKFYYDVLGLKVICDFGENVVLEGNISL
                     QEKKLWLEFINKSDSEVKFNGNDAELYFEEDNFDTFVERLSTMKDIDYVHLAIEHRWG
                     QRAIRFYDLDGHIIEVGETMSSVCRRFLDSGLSIDEVAKRMDVTVEYIESVLE"
BASE COUNT          421 a           71 c          170 g          414 t
ORIGIN      
        1 aactaaatta gaagaactaa aaactatatt tttattaatt ataatatttg aataatcttt
       61 ttaatcatat aaaatgcata tacttgtaat atttaaaggt gatattaata ttattagtgt
      121 atattaatat tcccttgaaa ttaaaaaaac atagatattt cagtaatcta aaatagtcta
      181 caaaaataac agttttagca aaattaataa gaatacatat agatttaaac ctaaaagtat
      241 gaacatgtct gttttttaca agattaaaat ttatattata tgataaaatt aacttaacta
      301 tttcaataag acttataatt tttttcttta tactagacct tattgaaacc tttatagttt
      361 aattctaatt atatattata aataaagtat gtgtatatta aggaggtaat atgaagtttt
      421 taaatgtttt agtagtagtt gaagatattg agaaatcaaa aaagttttat tatgatgttt
      481 tggggttaaa ggtaatctgt gattttggag aaaatgttgt attagaaggt aatatatcat
      541 tacaagaaaa aaagctttgg ttagaattta ttaataagag tgacagtgaa gttaagttca
      601 atggaaatga tgctgagtta tattttgagg aagataattt tgatactttt gttgaaagat
      661 tgagtacaat gaaagatata gattatgttc atctagctat agagcatagg tggggtcaaa
      721 gagcaattag attttatgat ttagatgggc atattattga ggttggagaa actatgtcat
      781 ctgtgtgtag acgtttttta gatagtggtc tttcaataga tgaggtagct aaaagaatgg
      841 atgtaactgt cgaatatatt gaatcagttt tagaatagat ggattatatt aaagtaaaat
      901 tgaggtgaag gctgtaagta aacttgtgtg aaaacaaatt tgcttacagt ttttattttt
      961 ctaaaataaa gaatataata cttgtttagc tttatttgag tttttaaaaa tattatgata
     1021 tttcttatat atttatacta aagtttaaat ttagaaaaaa tatataaaag atagtt
//
LOCUS       DAELLV010000422         1065 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.426, whole genome shotgun sequence.
ACCESSION   DAELLV010000422 DAELLV010000000
VERSION     DAELLV010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.426"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            181..>1065
                     /locus_tag="KPD58_003711"
     CDS             181..>1065
                     /locus_tag="KPD58_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG0267401.1"
                     /translation="MKINKKFNRVDMQVSILTAIIVIISSSCVYFFNYTLTYKSMIYS
                     LSERSKHIYEYVERNIDKNTFTEINSKEDQSKKSYKSSKILLESVKNTTGVMYLYTAK
                     KTKDGSLVYVVDGLNSSRSDFRNAGDLIEKEIWGDLNKALDNHILLPKKIKSTSWGYI
                     FISYFPIHNDVGKVVGVIGIEFEAKHQYNTFKFISIVTPIIALLTCLISALIAVILFK
                     RISNPTYTDFANTDYLTGLKNRNAFEIDMNNLNNSNLKNLISIISIDLDGLKKINDKF
                     GHQTGDLYIKQASKIIQYV"
BASE COUNT          434 a          117 c          149 g          365 t
ORIGIN      
        1 ttatacataa aatagtatac tataataata aaaaaactat atttgaatgc tttaagcatg
       61 tttactaatt gtaatttcca cacaaatttg ataatatatg aatatattat ggtgaaattt
      121 atagatatat attaattatg gggagattgt gggaggttct tttcacaaga catttaagct
      181 atgaaaataa ataaaaaatt caatagagtt gatatgcagg tttcaatact tactgcaatt
      241 attgtaataa tatcttcctc atgtgtatat ttttttaatt atacactaac ttataagagc
      301 atgatatact ctttaagtga gaggtcaaaa catatctatg aatatgtaga aagaaatatt
      361 gataaaaata cctttactga aattaattct aaagaagacc aatcaaaaaa gtcttataaa
      421 agttctaaaa tccttttaga atctgtaaaa aatactactg gagtcatgta tctatatact
      481 gctaaaaaaa caaaagatgg atcactagta tatgttgttg atggtttaaa ttcatctagg
      541 agtgatttta gaaatgctgg tgatttgata gaaaaagaga tttggggaga ccttaacaaa
      601 gcattagata atcatatatt gttacctaaa aagattaaat caacatcttg gggatacata
      661 tttatatcgt attttcctat tcacaatgat gttggtaagg ttgttggagt aataggaatt
      721 gaatttgaag caaaacatca atacaataca tttaaattta taagtatagt tacaccaata
      781 atagcattat taacttgtct aatatcagca ttgattgcag tgattctatt taaaagaata
      841 tctaatccta catatactga ttttgctaat acagattatt taacaggcct aaaaaataga
      901 aatgcttttg aaatagatat gaataactta aacaattcaa atctaaagaa tctaatatct
      961 atcatatcaa tagatttaga cggtttaaaa aagattaatg ataaatttgg ccatcagaca
     1021 ggagacttat atattaagca agcaagtaaa attatacaat atgta
//
LOCUS       DAELLV010000423         1061 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.427, whole genome shotgun sequence.
ACCESSION   DAELLV010000423 DAELLV010000000
VERSION     DAELLV010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.427"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            39..155
                     /gene="rrf"
                     /locus_tag="KPD58_003712"
     rRNA            39..155
                     /gene="rrf"
                     /locus_tag="KPD58_003712"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            163..237
                     /locus_tag="KPD58_003713"
     tRNA            163..237
                     /locus_tag="KPD58_003713"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:195..197,aa:Asn,seq:gtt)
     gene            243..328
                     /locus_tag="KPD58_003714"
     tRNA            243..328
                     /locus_tag="KPD58_003714"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:277..279,aa:Leu,seq:taa)
     gene            344..419
                     /locus_tag="KPD58_003715"
     tRNA            344..419
                     /locus_tag="KPD58_003715"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:377..379,aa:Met,seq:cat)
     gene            427..501
                     /locus_tag="KPD58_003716"
     tRNA            427..501
                     /locus_tag="KPD58_003716"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:460..462,aa:Glu,seq:ttc)
     gene            516..589
                     /locus_tag="KPD58_003717"
     tRNA            516..589
                     /locus_tag="KPD58_003717"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:548..550,aa:Gly,seq:tcc)
     gene            595..670
                     /locus_tag="KPD58_003718"
     tRNA            595..670
                     /locus_tag="KPD58_003718"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:628..630,aa:Val,seq:tac)
     gene            676..752
                     /locus_tag="KPD58_003719"
     tRNA            676..752
                     /locus_tag="KPD58_003719"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:710..712,aa:Asp,seq:gtc)
     gene            763..837
                     /locus_tag="KPD58_003720"
     tRNA            763..837
                     /locus_tag="KPD58_003720"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:795..797,aa:Gly,seq:gcc)
     gene            847..920
                     /locus_tag="KPD58_003721"
     tRNA            847..920
                     /locus_tag="KPD58_003721"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:881..883,aa:Arg,seq:tct)
BASE COUNT          285 a          245 c          265 g          266 t
ORIGIN      
        1 tattaaaaac ttaatagaaa tattaagcat aaagattatg tggttacaat agcagagagg
       61 atacacctgt tcccattccg aacacagaag ttaagctctc tagcgctgat ggtacttggt
      121 gggaaactgc ctgggagagt aggacgtagc cacgttaaat gttccaaggt agctcaacgg
      181 tggagcaacc ggctgttaac cggtaggctg tgggttcgag ccccaccctt ggagccaaaa
      241 aagccgaagt ggcggaactg gcagacgcac aggacttaaa atcctgcggg acttacctct
      301 cgtaccggtt cgattccggt cttcggcact tataaaacaa tatcgcgggg tggagcagtt
      361 ggcagctcgt cgggctcata acccgaaggt cgtaggttca agtcctgcct ccgcaaccat
      421 taaaacggct cgttggtcaa gaggttaaga caccgccctt tcacggcggt aacaggggtt
      481 caattcccct acgagtcacc aattcaatta aatatgcggg tgtagctcaa tggtagagtt
      541 ctggccttcc aagccagctg tgagggttcg atccccttca cccgctccaa aaatgggacc
      601 atagctcagc tgggagagca cctgccttac aagcaggggg tcacaggttc gagccctgtt
      661 ggtcccacca ttcgcggcct ggtagttcag ttggttagaa tgccagcctg tcacgctgga
      721 ggtcgagggt tcgagcccct tccaggtcgc caaattaaat aagcgggaat agttcagtgg
      781 tagagcgcaa ccttgccaag gttgaagtcg cgagttcgaa tctcgtttcc cgctccataa
      841 taacttgggt gcatagctca gctggataga gcaacgccct tctaaggcgt gtgtccgggg
      901 ttcgaatccc tgtgcgctca ttaagaaaag tttactataa attatagtaa acttttatat
      961 tgtttaaaaa gacacaggaa acatgtatat tacctgtgtc tttttattta taaactttaa
     1021 actatgtaaa aattacagta tattctaata gagttacagt a
//
LOCUS       DAELLV010000424         1051 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.428, whole genome shotgun sequence.
ACCESSION   DAELLV010000424 DAELLV010000000
VERSION     DAELLV010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.428"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            226..600
                     /locus_tag="KPD58_003722"
     CDS             226..600
                     /locus_tag="KPD58_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0267402.1"
                     /translation="MKEEILIEKLLRAELIVMEYLWKKDTLMPKKEIVKEIKQIYGWH
                     KSTIKILLKRLVAKRYLARYIINSQSHYKIIDSKEYYTFKKKVLKSSKSIKIMRSLTT
                     THKNVSKEKLDLLDEYYRNLKE"
     gene            705..1051
                     /locus_tag="KPD58_003723"
                     /pseudo
     CDS             705..1051
                     /locus_tag="KPD58_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
BASE COUNT          478 a           94 c          151 g          328 t
ORIGIN      
        1 ttctaacgat ttagtgctat tctacaacta aaataataca atagagctat ttattgtttg
       61 gtagcattgt ttgcaaatta aacaaattta tatatatgaa tattatttta cgttcaaaaa
      121 aacagactaa atatgtagaa tattcggtaa aacttacctc tagtttttgt cttaaaaagc
      181 tcctattatt aacttaagtt agaagaatta aggaagtgac ataaattgaa agaagaaata
      241 ttaatagaaa aattattaag agcagaatta atagtaatgg agtatttatg gaaaaaagac
      301 actctaatgc ctaaaaaaga aattgtaaaa gaaataaaac agatttatgg atggcataaa
      361 agtacaataa aaatactact aaaaaggcta gttgctaaaa ggtatttggc tagatatatt
      421 ataaattctc aatctcatta taaaataata gatagtaaag aatattatac ttttaagaaa
      481 aaggtactaa aatctagtaa aagcataaaa ataatgcgtt cacttaccac tacacataaa
      541 aatgtaagta aagagaagtt agacctatta gatgagtatt atagaaactt aaaagaatga
      601 tatagtaaaa atcgaatgta attatttacc ttaattcttt tgtttgtaaa actttattga
      661 tacataattt aacatataca taaattggta aaggaatgta tactatgaaa attagtaaat
      721 taccagaagc ggaattaaaa gttatgagat atatttggga atcagaaaag gtattaatat
      781 caagagaaat caccaagtct atggaacaaa aatatgaatg gaaagataca actatattta
      841 cagtattaaa aagattacta aagaagagag tttttagaaa cggagaaaat agataagtgt
      901 acacactata atgtattagt aaaaaaaaat aatatcagag attttaaaca aaagaatttt
      961 taaagaatac acataaaaat tcaatattaa gtttaatgtc agcactatgt agtaaggatg
     1021 atgaagtaga tggtgatatt ttaaatttat t
//
LOCUS       DAELLV010000425         1018 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.429, whole genome shotgun sequence.
ACCESSION   DAELLV010000425 DAELLV010000000
VERSION     DAELLV010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.429"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..680
                     /locus_tag="KPD58_003724"
     CDS             <1..680
                     /locus_tag="KPD58_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBG0267403.1"
                     /translation="FGGISFSEIFLVVGIVLIIYQIFKKKIKEKKVFYRVLKIIISIF
                     LIVFVVTESLIIFYPKNSLESKSDYLLILGASVKKTTPSTTLKGRLDTALKYLKINDN
                     CYVVVSGGKGSGEKITEAKAMKDYLIKNGIDKNRIIEEDKSTNTYENFKYSKLKIEEH
                     SQGKLSDLKVKIVTTDFHVLRSKILAYRNGYKNTSFYASKSKLSFVPTYYTREFFALW
                     KTIIFDR"
     gene            complement(629..937)
                     /locus_tag="KPD58_003725"
     CDS             complement(629..937)
                     /locus_tag="KPD58_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBG0267404.1"
                     /translation="MNFFKENSTFYFATVEDNKPRVRPFGFIMDYEGKLYFGIVKYKT
                     SYKQLLVNPNVEISTTSKVGKWIRIKGIEKTRYEFISSLFIYYLSKMIVFHNAKNSLV
                     "
BASE COUNT          406 a          111 c          139 g          362 t
ORIGIN      
        1 tttttggagg aatttcattt agtgagattt tcttagttgt aggaattgta ctaattatct
       61 atcaaatatt taagaaaaaa attaaagaaa agaaggtttt ttatagggtg ttaaaaataa
      121 taatcagcat ttttttaata gtatttgtag ttactgagag tctaattatt ttttatccca
      181 aaaatagttt agaatcaaaa agtgattatc tcttaatttt aggagcttct gtaaaaaaga
      241 ctacaccaag tacaaccctg aagggtaggt tagacactgc attgaaatac ctaaaaatta
      301 atgataattg ttatgtggta gtatctggtg gtaaaggaag tggagaaaaa ataactgagg
      361 caaaagctat gaaagattat cttataaaaa atggtataga taaaaatagg ataatagaag
      421 aggataaatc cacgaatact tatgagaatt ttaaatattc aaaattgaag atagaagaac
      481 atagtcaagg aaaactaagc gatttaaaag taaaaatagt aacaacagat tttcatgttc
      541 tcagaagtaa aatattagca tatagaaatg gatataaaaa tacgtctttt tatgctagta
      601 aatcaaaact ttcatttgta ccaacttact atacaagaga attttttgca ttatggaaaa
      661 caatcatttt tgatagataa taaataaaaa gactagatat aaactcatat ctagtctttt
      721 ctattccctt aattctaatc cattttccaa ctttacttgt tgtagaaatt tcaacattgg
      781 gatttacaag taattgctta taagaagttt tatattttac gattccaaaa tataattttc
      841 cttcataatc cattataaat ccgaatggtc ttactcttgg tttattatct tcaactgtag
      901 caaaatagaa tgttgagttt tctttaaaaa aattcattat tttttcatga ttaaatcctc
      961 tttgtatctt atactaatat aatgaatata aataaaatat tcataatact aatataaa
//
LOCUS       DAELLV010000426         1014 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.430, whole genome shotgun sequence.
ACCESSION   DAELLV010000426 DAELLV010000000
VERSION     DAELLV010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.430"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            153..863
                     /gene="rgaR"
                     /locus_tag="KPD58_003726"
     CDS             153..863
                     /gene="rgaR"
                     /locus_tag="KPD58_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgaR"
                     /protein_id="HBG0267405.1"
                     /translation="MFRVVICDDEKIQRSILKEFTEKLLSERCLNYQILEFSCGEDLI
                     SKYPEKIDIIFLDIQMKDINGIEVAKKIRKFDEKVEIIFTTAFSEYAPQGYEVRAYRY
                     LVKPIEYSNFAMGVNLCIDNLQRKKERYIVLNSKKGFNRILIDSILYVETVKRDLVVH
                     TIGRDYKANMSMKQAEKLLCENGFFRCHTSFIVNLRRIEDLRDNMITINEQFIPISKY
                     RLKDLKVALTSLLCDIMY"
BASE COUNT          412 a           83 c          173 g          346 t
ORIGIN      
        1 taatttaagt ttaaatatta aagagaattt ctataagtag tttaatcgac aatttaaatt
       61 tataaagaaa tttaaatttg aaagctagtg agtacagttt ggaaattgaa ataaagtaaa
      121 ctaaatgata ttttagattt ggaggtgata ttatgttcag ggtagttata tgtgatgacg
      181 aaaaaataca aagaagcata ctaaaagagt ttacagaaaa acttttgtct gaaagatgtc
      241 taaattatca aatactggaa ttttcctgtg gagaagattt aatatccaaa tacccagaaa
      301 aaattgatat tatattttta gatatccaaa tgaaagatat caatggaatt gaggtggcta
      361 aaaaaataag aaagtttgat gaaaaagtag aaattatatt tacaacggct ttttcagagt
      421 atgcccccca aggttatgag gttcgtgctt atagatattt agtaaagcct atagagtata
      481 gtaactttgc aatgggtgta aatctttgta ttgataacct tcaaagaaag aaggagcgtt
      541 atatagtttt aaatagtaaa aaaggattta atagaatact gatagattct attttatatg
      601 ttgaaactgt aaagagagat ttagtcgtac atacaatagg tagagattat aaagctaata
      661 tgagcatgaa acaagcagaa aaattactgt gtgaaaatgg cttttttaga tgtcatacaa
      721 gttttattgt taatttaaga agaatagaag atcttaggga taatatgatt actattaatg
      781 aacaatttat acctataagt aagtatagac ttaaagattt aaaagtagca ttaacaagct
      841 tgttatgtga tataatgtat taattttaaa aaggtaattg aaaatatcgt aaaaaagatt
      901 taatgtcatc acagggctat tgattgaaac tgtcttaata tataaaatgt tatttatatt
      961 aagacagttt tattatgcaa aatagcagtt tatagatttt taatatggtt taac
//
LOCUS       DAELLV010000427          988 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.431, whole genome shotgun sequence.
ACCESSION   DAELLV010000427 DAELLV010000000
VERSION     DAELLV010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.431"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..>988)
                     /locus_tag="KPD58_003727"
     rRNA            complement(<1..>988)
                     /locus_tag="KPD58_003727"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          220 a          291 c          223 g          254 t
ORIGIN      
        1 ctattagtat cgataagcta aatacattgc tgcacttaca ccttcgacct atcaaccaga
       61 tagtctttct ggggtcttac ccttgcggtg ggaaatctta tcttgaagtt ggcttcgcgc
      121 ttagatgctt tcagcgctta tccattccgt acatagctac ccagccatgc ccttggcagg
      181 acaactggta caccagaggt acgtccatcc cggtcctctc gtactaagga caggtcttct
      241 caaatttcct acgcctgcga cggataggga ccgaactgtc tcacgacgtt ctgaacccag
      301 ctcgcgtacc actttaatgg gcgaacagcc caacccttgg gacctactac agccccagga
      361 tgtgatgagc cgacatcgag gtgccaaacc tccccgtcga tgtggactct tgggggagat
      421 aagcctgtta tccccagggt agcttttatc cgttgagcga tggcccttcc acgcagaacc
      481 accggatcac taagtccgat tttcatcctt gctcgacctg tatgtcttgc aatcaagctc
      541 tcttttgcct ttacactcta cgtacgattt ccgaccgtac tgagagaacc tttgagcgcc
      601 tccgttacct tttgggaggc gaccgcccca gtcaaactgc ccacctgaca gtgtcccaag
      661 accagattca tggcctatgg ttagagtccc agtactacaa gggtggtatc ccaaggatgg
      721 ctccgccaaa actggcgtcc tggtttcaaa gcctcccacc tatcctgtac atgtagtacc
      781 aagacccaat gtcaagctac agtaaagctc catggggtct ttccgtcctg tcgcaggtat
      841 ccggcatctt caccggaatt acaatttcac cgagtctgtt gttgagacag tgcccaaatc
      901 gttacgcctt tcgtgcgggt cggaacttac ccgacaagga atttcgctac cttaggaccg
      961 ttatagttac ggccgccgtt tactgggg
//
LOCUS       DAELLV010000428          981 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.432, whole genome shotgun sequence.
ACCESSION   DAELLV010000428 DAELLV010000000
VERSION     DAELLV010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..981
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.432"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(137..820)
                     /locus_tag="KPD58_003728"
     CDS             complement(137..820)
                     /locus_tag="KPD58_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HBG0267406.1"
                     /translation="MTSKSQMSESFFLCSLLAITGGFLDIYTYVVRGKVFANAQTGNI
                     VLFGLNIAEGNVKESFYYLIPILAFMLGVFIAEKIRKYFTNNKTKIHWRQVIIIIEMI
                     TILIVSFIPRGRFNMFVNVAISFICSMQVESFRKVNGNSFATTMCTGNLRSATETLFH
                     YIHTKNIFMIKKSLQYYAIIIFFISGAILGTFFTKIFVEKSILICFFILAVVFGIMFI
                     NKDDSFNEN"
BASE COUNT          383 a          153 c           77 g          368 t
ORIGIN      
        1 ttactatact taaaatactt ctattaactt atacattgct tactatattt taaattcttt
       61 aattaatcta tacaatattt gctatatata aagtatatca aataacagct tcattttttt
      121 gtaaatttac agaaagttaa ttttcattaa atgaatcatc tttattgata aacataattc
      181 caaagaccac tgccaaaata aaaaaacata ttagtattga tttttccacg aaaatttttg
      241 taaaaaaagt tcctaaaata gctccagata taaagaatat aataatagca taatactgta
      301 aactcttttt tatcataaaa atattcttag tatgtatata atgaaataaa gtttctgtag
      361 cacttcttaa atttccagta cacattgtcg ttgcaaaact attcccattt acttttctaa
      421 aactctctac ttgcattgaa caaataaaag aaatagcaac atttacaaac atattaaatc
      481 ttcctctagg aataaatgaa acaataagta ttgtaatcat ttcaattata atgataacct
      541 gacgccaatg aatctttgtc ttattatttg taaaatactt tcttatcttc tcggctataa
      601 atacacctaa cataaatgcc aaaataggaa tcaaataata aaatgattcc tttacattac
      661 cttctgctat attaagccca aacaaaacta tatttccagt ttgtgcatta gcaaagactt
      721 tacctcttac cacataagta taaatatcca aaaatcctcc tgttattgcc aacaaagaac
      781 ataaaaagaa tgattcagac atctgactct tgcttgtcat atataaacct ctcgctttct
      841 aatgatagat acaaaatcaa aatcatttta gattctattt ttcattgata taaaaaaatt
      901 atttatattt aaatattgtc tatatttata catcatttat atttatacat tatttgtatt
      961 tatatagcat tcatatttat t
//
LOCUS       DAELLV010000429          979 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.433, whole genome shotgun sequence.
ACCESSION   DAELLV010000429 DAELLV010000000
VERSION     DAELLV010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.433"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(145..651)
                     /locus_tag="KPD58_003729"
     CDS             complement(145..651)
                     /locus_tag="KPD58_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HBG0267407.1"
                     /translation="MGFREVKIEELQFNPFTKIGKEWLLITAGDSEKFNTMTASWGGV
                     GVYWGKNVVTTYIRPQRYTKEFVDSNDTFTIAFFDETYREALNICGTISGRDINKIEK
                     AGLTPYFVDDTVAFEEANMIIVCKKLYHGNMPPENFDAKENDKKWYPEKDYHTMYISE
                     IIKVLVKE"
BASE COUNT          337 a          144 c           92 g          406 t
ORIGIN      
        1 tatagtaaat atagttcatc ttttttataa tagtatttat ttaaaattaa tatttattta
       61 gttagtagca tctacttaga tatcagataa gaaagcacag tcatttactt ataataagtt
      121 cttttcatat attttttatt ataattattc ttttacaaga acttttataa tttctgaaat
      181 gtacatagta tgatagtctt tctctggata ccatttttta tcattttctt tagcatcaaa
      241 gttttctggt ggcatattac catgatatag ctttttacat actataatca tattagcttc
      301 ttcaaaagct accgtatcat ccacaaaata tggtgtaagt cctgcttttt ctattttatt
      361 tatatctcta ccagaaatag tgccacaaat atttaaagct tctctatacg tttcatcaaa
      421 gaaggcaatt gtaaatgtat cattactatc tacaaattct tttgtatatc tctgtggtct
      481 tatgtaagtt gtaacaacat ttttacccca atagactcca acaccacccc agctagcagt
      541 cattgtgtta aacttttctg aatcaccagc tgttataagt aaccattctt tgccaatctt
      601 agtaaatgga ttaaactgta attcctcaat ttttacttct ctaaaaccca tgacttcatc
      661 ctacccttca ttttttaatt atataaatta attgttaaaa atttgaatat atgtactctt
      721 ataatactga cttttgattg tcctacataa ttcatgtaaa ttataataca acaaatcaat
      781 tactttcgca ataagtagtt ttcatatttt ataagtgtaa aatatagata aaatatattg
      841 attataaagt atataatata tttttaaacc taaagcaata tacaataaaa aaatgtagaa
      901 agtaagtatc tctctacatt ttttattgta tattattttt atattaattt aattttatct
      961 tgattcagtt ttataaaaa
//
LOCUS       DAELLV010000430          927 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.434, whole genome shotgun sequence.
ACCESSION   DAELLV010000430 DAELLV010000000
VERSION     DAELLV010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.434"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            92..862
                     /locus_tag="KPD58_003730"
     CDS             92..862
                     /locus_tag="KPD58_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBG0267408.1"
                     /translation="MSKGFLTPGETAQATISAGIKKANISTQNAILLGLFGGAFIGFG
                     ALGSMIVSQTFGKIDPGVASFLSAMVFPVGLMLVVVAGAELFTGNTLMAIALMDKKIT
                     LGKMLRNWGLVYFANLVGSILLVALVFYSSTLTGDAATKAIAVAEAKVNSTIASMFLK
                     AILCNILVVLAVWMATASQDIMSKLASCWTVIMLFVLCGFQHSVANMFFIPMGMALGA
                     NITMVQFITNLVFVTLGNIVGGSIVVGGIYYLCYVKNS"
BASE COUNT          305 a          113 c          190 g          319 t
ORIGIN      
        1 ttaataaaat ttattatata tcatattgtt attatataat tattgccata aaaagacaag
       61 ggaaagtata atttttaagg gggaaggatt tatgagtaaa ggattcttga cacctgggga
      121 aactgcacaa gcaactataa gtgcaggcat taaaaaagca aatatttcga cacaaaacgc
      181 aattctatta ggtttatttg gaggagcatt tataggtttt ggggcattgg ggagtatgat
      241 agttagtcaa acttttggca agatagaccc aggggtagct agctttttaa gtgctatggt
      301 ctttccagta ggtcttatgc ttgtagtagt ggcaggagca gaattattca ctggaaacac
      361 cttaatggct attgcactta tggacaaaaa aataacatta ggaaaaatgt taagaaactg
      421 gggactggtt tattttgcaa atttagtagg ttcaattttg ctagttgcac ttgtgtttta
      481 ttcttctaca ttgacaggag atgcagctac aaaagctata gctgtggcag aagctaaggt
      541 taattcaact atagcatcaa tgtttttaaa agcaatactt tgtaatattc tagtcgtttt
      601 agctgtatgg atggctacag catctcaaga tataatgtca aaattggctt catgttggac
      661 agtaataatg ctatttgtat tatgtggatt ccaacatagt gttgcaaaca tgttttttat
      721 accaatggga atggcattag gagcaaatat aactatggtt caatttataa ctaatttggt
      781 atttgtaaca cttggaaata tcgttggagg ttccatagta gttggaggga tttattatct
      841 ttgctatgtc aaaaatagtt agataaataa tacgaattaa attgcctttt aaagcattga
      901 tatcagtgat attgatgctt tttttaa
//
LOCUS       DAELLV010000431          904 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.435, whole genome shotgun sequence.
ACCESSION   DAELLV010000431 DAELLV010000000
VERSION     DAELLV010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.435"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..608
                     /gene="rgbR"
                     /locus_tag="KPD58_003731"
     CDS             <1..608
                     /gene="rgbR"
                     /locus_tag="KPD58_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component system response regulator RgbR"
                     /protein_id="HBG0267409.1"
                     /translation="YISGEELLESNLKDIDILLLDIKMEKLNGMDTARKIREVDNEME
                     IIFVTSLIDYVQEGYEVRAYRYLLKPIELEELKKHVLTCIKDIEINKESHITIKNKSN
                     TYKIYLNEIKYIEVQKKDMLIHTINKNFDIKYSLGKIEKELNPYKFIRCHKSFIVNLR
                     YVENIKPNTAILESGEEVPISRYRYKEVKEKFLKFLGDTIC"
BASE COUNT          389 a           65 c          139 g          311 t
ORIGIN      
        1 agtatatatc tggagaagag ttactggaaa gtaatttaaa agatatagat atattacttc
       61 ttgatataaa aatggaaaaa ttaaatggaa tggatactgc aagaaagatt agagaagtag
      121 acaatgaaat ggaaataata tttgtaactt cattaataga ttatgtgcaa gaaggttatg
      181 aggtaagagc ttataggtat ttactaaagc caatagagtt ggaagaacta aaaaaacatg
      241 tgctaacttg tatcaaagat attgaaataa ataaggaaag tcatattaca ataaaaaata
      301 agtctaatac atataagatt tatttaaatg aaataaaata tatagaagtt caaaaaaaag
      361 atatgctaat acacacaata aacaagaatt ttgatataaa atatagttta ggcaaaatag
      421 aaaaagaatt aaatccatat aaatttataa gatgccataa aagttttata gtaaatttaa
      481 gatatgttga aaatataaag cctaatactg caatactgga aagtggagaa gaggtaccta
      541 ttagtagata tagatataaa gaagttaagg agaaattctt aaaatttctt ggtgatacaa
      601 tatgctaaac tttatgactg agctattagt agttttatct atatttatac agttttctat
      661 aatgaacgga attttaattt ataaaaagga tagtaaaaaa aatgaaaaaa tagtattcta
      721 tattgtcttg tttcttattt atattataat tcagataagt agattattta atggaggaaa
      781 ttactttagt aatataaact atttatttta tatattagca tttattgctt ggggaggata
      841 ctataattat tttttataag atgggttata ttaagtattc cattttcttt tatctattta
      901 gtgt
//
LOCUS       DAELLV010000432          890 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.436, whole genome shotgun sequence.
ACCESSION   DAELLV010000432 DAELLV010000000
VERSION     DAELLV010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.436"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            56..433
                     /locus_tag="KPD58_003732"
     CDS             56..433
                     /locus_tag="KPD58_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0267410.1"
                     /translation="MTITKIPPAELKVMKFIWSSDFTVASKDVIEAMEKLYNWKQTTT
                     LTLLSRLVNKQFLSARKIDRHTHYTVLIGEEEYLHFETKTFLDNMHGSSIESFMKSLF
                     KKGVNEEELNSVEKWVKDIKKDE"
BASE COUNT          347 a           64 c          136 g          343 t
ORIGIN      
        1 agtctattaa tatagtattt aattatgtag aagtttgaga ggagtgtaaa atcatatgac
       61 tattactaag ataccaccag cagaattaaa agtcatgaaa tttatttgga gttcagattt
      121 tacagtagca tcaaaagatg tcattgaagc tatggaaaaa ctatataatt ggaagcaaac
      181 tacaacacta acacttttat caagattagt aaataagcaa ttcttaagtg caagaaaaat
      241 tgatagacat acacattata cagttcttat tggagaagaa gagtatttac attttgaaac
      301 aaaaactttt ttagataata tgcatggaag ttctattgag agttttatga aatccttgtt
      361 caaaaagggg gtaaatgaag aagaattgaa ctctgttgaa aaatgggtta aggatattaa
      421 aaaagatgaa taatttggtg tagttaatta tcataaaata aatagttttt acaataatta
      481 gttaaatgaa tcaaatataa agttttatgt aaaatttgtg atttttataa atatttttgt
      541 cttgttattt aatattaata caataagttt gatgtaaaat caaacctgtt tgaatttaat
      601 ttatattggg tttgtcatta atattatttt agtttgatat tgtatacgaa tttaaaagtc
      661 agaatttttg tatattaatc atgtaaatta attgttttag atataaaggg cataatttta
      721 tttttgtttt gattatttct agggtaagat aagtatacaa tagattgtat taaacatgct
      781 tatattgata gatttgagat aaatattgag atatattaat aattattaat ataaaaaagt
      841 tcctagtttt taggaacttt tttatttgat taatacagag tatgagtttt
//
LOCUS       DAELLV010000433          890 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.437, whole genome shotgun sequence.
ACCESSION   DAELLV010000433 DAELLV010000000
VERSION     DAELLV010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.437"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
BASE COUNT          362 a          102 c           87 g          339 t
ORIGIN      
        1 taattagact tctctcttat tcaagatttt tctatataaa atctaagaat aaatagaaaa
       61 ctaacatcta accttttatt ttttattttc gatctattta cttgaatttt aacataaatc
      121 tatcttttag tatatattaa tcatgctgaa tttgtgatga catgtataca aaatatcgtt
      181 tagtattttc taataaaaaa tattactgaa attaaaaatc tacaatagca tttaacaagt
      241 aaaaacatgt ttacacaaat atataattta aggtctttaa aatagagtat gtattattct
      301 aaaaattagt tatagttatc acataaaaat agaaaagaat tttcaaaaga tttaaagaac
      361 atacatactt caattgcaga agatgtaatg ttaagtgaac tagcattctt cgaggaaaaa
      421 tagactcaaa aaattagaaa atagaatcag gttttagatg atttaagtat ttattttcaa
      481 aaaagaaatt tgattctaag ttaataaaga aatatttaaa aaaatcaaaa aattttctta
      541 aataagtaat aggttttgta aaaatggaaa aatatcaaaa taagactatg atttttaaaa
      601 ctatagcctt atcaataaat ttttatttaa agaacacaca taaatatttt taaacctaat
      661 ttatgtgaag ttttatgtat tttttatata agtagtattc taatcttact catctaatat
      721 accaaaaaga ctgtaagtaa gttcactttt atgcaaactt acccacaatc tcttttacac
      781 attaattcta tttattaaaa ttaaaaatat ttactgctat tctacccaat aaagatgata
      841 ctgttgttgt tctggctggt actgttactt ctatttctcc tgtatctgct
//
LOCUS       DAELLV010000434          873 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.438, whole genome shotgun sequence.
ACCESSION   DAELLV010000434 DAELLV010000000
VERSION     DAELLV010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.438"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            7..498
                     /locus_tag="KPD58_003733"
     CDS             7..498
                     /locus_tag="KPD58_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0267411.1"
                     /translation="MYENNKVIYNLERCEKLEKTLYLSQFNTYKNFKLASKKTVINSL
                     ETYVYNMNINNISLVDTIECISLEYEQLTGKKLILVGDIDIDAILDYAGNKRNRNSSK
                     KNTFKLKIPFSTFIQMPRKIENKDKINLKYLIQDITSSILDDNLFISVTAIISYENSS
                     KIE"
     gene            526..>873
                     /locus_tag="KPD58_003734"
     CDS             526..>873
                     /locus_tag="KPD58_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0267412.1"
                     /translation="MRITKTTFNDVFSNDANGSIIKVEKYVDKKCASVFEILTYTIIV
                     TNTSRYKTGNIFFKDYISKYIEFINNTVKVNGIIKRGLDPQQGFYIGRIDASCKKIIS
                     FKSVVLPNSAHRII"
BASE COUNT          378 a           78 c          123 g          294 t
ORIGIN      
        1 gcgattatgt atgaaaataa taaggtaata tacaaccttg aaagatgtga aaaactagaa
       61 aagactcttt atttatctca atttaataca tataaaaatt ttaaattagc ttctaaaaaa
      121 actgtaataa attcattaga gacatatgtt tacaatatga atattaacaa tataagtctt
      181 gttgatacta tagaatgtat atcattagaa tatgaacaac ttacaggaaa gaagttgatt
      241 ttagttggtg atatagatat tgatgcaata ttagattatg caggaaataa aagaaataga
      301 aattctagta aaaaaaatac atttaaatta aaaataccct ttagtacatt tatacaaatg
      361 cctagaaaga tagaaaataa agataaaatt aatttaaagt atcttataca agatattact
      421 tcatctattt tagatgataa tttatttatt agtgttactg caattataag ttatgaaaat
      481 agttccaaga tagagtaatc tttaaattat agaaaggtgg atgcaatgag aataacgaaa
      541 actactttta atgatgtatt ttcaaatgat gcaaatggaa gtattataaa agtagaaaaa
      601 tatgttgata aaaaatgtgc atctgtattt gagattttga cctacactat tattgttaca
      661 aatacaagta ggtacaaaac tggaaatata tttttcaaag attatatttc taaatatata
      721 gagtttataa ataatactgt aaaagttaat ggcattataa aacgtggatt agacccacaa
      781 caaggttttt acatagggag aatagatgca agttgtaaaa aaataatatc ttttaagagt
      841 gtagttttac caaattctgc acataggatt ata
//
LOCUS       DAELLV010000435          856 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.439, whole genome shotgun sequence.
ACCESSION   DAELLV010000435 DAELLV010000000
VERSION     DAELLV010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.439"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(96..806)
                     /locus_tag="KPD58_003735"
     CDS             complement(96..806)
                     /locus_tag="KPD58_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0267413.1"
                     /translation="MINIGICDDELHYRIKIKDILSEILSSYPINYNIYEFSAGEELL
                     NNYPKDLDILIMDIQMKTINGMDTARKIREFDHKLEIIFVTSFVEFMQEGYEVKAYRY
                     ILKPINKEKISKSVLPCINEMMKKRNNYLTINVKNYVDRIKIDSITYIETDRPNILIY
                     THDDMYITKMSISKIEKILNEYGFFRCHNSYIVNLKLVESMSGSTVIVDGKSIPISKY
                     RVKGLKLAITNILGDIVC"
BASE COUNT          287 a          138 c           63 g          368 t
ORIGIN      
        1 ctttccaact aagtggatta ctaaaaaaaa tataatccaa tctatagtac ctgaaactaa
       61 attaataata ttccaaaatc ctacacttaa atttgttagc atactatgtc ccctaatata
      121 ttagttatcg caagcttcaa gcctttaacc ctatatttac ttattggaat actttttcca
      181 tctactataa cagtacttcc actcattgat tctactaatt ttaagttaac tatatagcta
      241 ttatgacatc taaaaaatcc atattcattt aatatctttt ctattttaga tatactcatt
      301 ttagtaatat acatatcatc atgtgtataa atcaatatat tcggtctatc tgtttctata
      361 taagtaatag aatctatttt aattcggtct acataatttt ttacatttat agttaagtaa
      421 ttatttctct ttttcatcat ctcatttata caaggtaata cacttttaga aattttttct
      481 ttatttattg gctttagtat atatctgtat gcttttacct cataaccttc ttgcataaac
      541 tctacaaatg aagtaacaaa tattatttct aacttatgat caaactctct tatcttccta
      601 gcagtatcca taccatttat tgttttcatt tgtatgtcca taattaatat gtctaaatct
      661 ttaggatagt tatttagtaa ctcttctcct gctgaaaatt catatatgtt ataatttata
      721 ggatatgaac ttaatatttc acttaatata tcttttattt ttattcgata atgtagttca
      781 tcatcacata ttcctatatt aatcacaatt cttaaacatc ctttcttata tatttttaat
      841 aaaaatatat aaattt
//
LOCUS       DAELLV010000436          831 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.440, whole genome shotgun sequence.
ACCESSION   DAELLV010000436 DAELLV010000000
VERSION     DAELLV010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.440"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            63..761
                     /locus_tag="KPD58_003736"
     CDS             63..761
                     /locus_tag="KPD58_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0267414.1"
                     /translation="MEKKYFEIHGVPSILWGNPSSKLYIYIHGQCGNKEEAEAFANIA
                     VPHECQVLSVDLPEHGERKREKNSFNPWHVVPELVSIMEYAKCHWNQISLYANSIGAW
                     FSMLSFEKENLRECIFVSPILDMQQLISNMMLWANVSEEQLKHELIIPTSFGHTLSWE
                     YLQYVKQHPITQWNVPTKILYGKHDELTEIGVVEKFVEKFNCNLTIIENGKHWFNTPQ
                     ELNSLYEWLNTLVK"
BASE COUNT          313 a          106 c          130 g          282 t
ORIGIN      
        1 cattatttta tagtttgatt aatttaacaa tcactaatat atcaaactga aaggagattt
       61 ttatggaaaa aaaatatttc gaaattcatg gagttccttc aatcctttgg ggaaatcctt
      121 caagtaaatt atatatttac atacatggac aatgtggcaa taaagaggag gctgaagctt
      181 ttgctaatat tgcagttcca catgagtgtc aagttttaag tgttgatttg cctgaacatg
      241 gagaacgtaa aagagaaaaa aattcattta acccatggca tgttgttcca gaattagtat
      301 ctattatgga atatgcaaag tgccactgga atcaaatttc gttatatgca aatagcattg
      361 gagcatggtt tagtatgtta agctttgaaa aagagaactt aagagagtgt atttttgtat
      421 ctccaattct agatatgcaa cagctcattt caaatatgat gctttgggca aatgtatcag
      481 aagaacagct taaacatgaa ctaattatcc caacttcatt tgggcatact ctttcatggg
      541 aatatctaca atatgtaaaa caacatccta tcactcaatg gaatgtacca acaaaaatat
      601 tatatggtaa acatgatgaa cttactgaaa tcggtgttgt agagaaattt gtagaaaagt
      661 ttaattgcaa ccttactatt atagaaaatg gcaaacattg gtttaacaca ccacaagaat
      721 taaattcatt atatgaatgg ttaaatactt tagttaaata aaaaataatt atattattaa
      781 tatgattaaa ttatttataa atgttattta attaatttta ttttagcatt a
//
LOCUS       DAELLV010000437          829 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.441, whole genome shotgun sequence.
ACCESSION   DAELLV010000437 DAELLV010000000
VERSION     DAELLV010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.441"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(19..744)
                     /locus_tag="KPD58_003737"
     CDS             complement(19..744)
                     /locus_tag="KPD58_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBG0267415.1"
                     /translation="MKTISKQELEKTLEKHYLWLKNNNAGEKADLKELRLENMDLRGY
                     CLSNIDFSWSDLINLNLEKADLENSIFNNSYLSDCSLKNSILKNADLSGASFRFCDLS
                     NSDIRGANLENSNLEHATLNGVISDESTRFFKLYCPETGAFIGYKKCFNFRMVQLLIP
                     SDAKRVSATSNACRCDKAKVLSITSIDGKESFKSARAYADENFIYRVGETIVAEDFNE
                     NRWKESTTGIHFFLTREEAIGYL"
BASE COUNT          286 a          137 c           84 g          322 t
ORIGIN      
        1 ttataaaact ttagcatttt atagataacc aatagcttcc tctctagtta aaaaaaagtg
       61 aatccctgta gtcgattctt tccatctatt ttcattaaaa tcttcagcta caatcgtttc
      121 tcctactcta tatataaaat tttcatcagc atatgcacga gcacttttaa aagattcttt
      181 accatctata ctagtaattg acagtacttt tgccttatca catctacaag catttgatgt
      241 agcagacact ctttttgcgt cgctgggtat taataattgt accattctaa aattaaaaca
      301 ttttttatag cctataaatg ctcctgtttc aggacaatac aatttaaaaa aacgggtact
      361 ttcatctgat attactccat ttaaagtagc atgttctaaa tttgaatttt ctaaattagc
      421 tcctctaata tctgagttag ataagtcaca aaatctaaaa cttgcaccac ttaagtctgc
      481 attttttaat atagaatttt ttaatgaaca atccgaaaga taagaattat taaaaataga
      541 gttttctaaa tctgcttttt ctaaatttaa attgataagg tcagaccacg aaaagtcaat
      601 attacttaag cagtatcctc ttaagtccat attttctaat cttaattctt ttaaatctgc
      661 cttttctcca gcattattgt ttttaagcca taaataatgt ttctcaagtg ttttttctaa
      721 ttcttgttta gaaatagttt tcattaaaat caccttcata aatttatttt tatatatgta
      781 gtagcaaaat agtcatatat ttaattatat aacaataaat aaaaaactt
//
LOCUS       DAELLV010000438          817 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.442, whole genome shotgun sequence.
ACCESSION   DAELLV010000438 DAELLV010000000
VERSION     DAELLV010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..817
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.442"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            61..663
                     /locus_tag="KPD58_003738"
     CDS             61..663
                     /locus_tag="KPD58_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HBG0267416.1"
                     /translation="MDNLIRILILAVIGGFIGYVTNVVAIRLIFRPIEPIKIPILNIE
                     IVGLIPKRRAEIAANVGEIIQEEFLSMDEILANIITDEDKEEVVRYIKARVKIIIHEK
                     VSFIPSGIKNMIQDYLGEIIESEVKQSIDELSKNIINKANERIDIQKMVEDKINELDL
                     YELEEIIIRIAKKELKHIEFLGLVLGFLIGIAQGLITMFI"
BASE COUNT          334 a           68 c          142 g          273 t
ORIGIN      
        1 atctacttga ttaatacaat atagctggca aatatacaat tgataaggag taattttata
       61 atggataatt taattaggat actgatatta gctgttattg gtggatttat aggttatgtt
      121 acgaatgtag ttgctataag actgatattt agaccaatag agcctattaa aatacctatt
      181 ttaaatatag aaatagtagg tcttatacca aagagaagag cagaaatagc agcaaatgta
      241 ggagaaataa ttcaagaaga atttctttct atggacgaaa tacttgcaaa tatcattact
      301 gacgaagata aggaagaagt tgtaagatac ataaaagcaa gggtaaaaat tataatacat
      361 gagaaagttt catttatccc gagtggtata aaaaacatga tacaagatta tctaggagag
      421 ataattgagt ctgaagttaa gcaaagtata gatgaattga gtaaaaatat aataaataaa
      481 gcaaatgaac gtatagatat acaaaaaatg gtcgaagata agattaatga actagattta
      541 tatgaattgg aagagataat aataagaata gctaagaaag aattaaaaca tatagagttt
      601 ttggggcttg tcttagggtt tttaataggt atagctcaag ggttaattac tatgtttatt
      661 taaatatatg agaagtagca tttatttaag tactaattat atttacctat ttgagtgaat
      721 tacttattaa taacatttag atattaattg tatttaccta tttgagtaaa ttacttatta
      781 atagcattta gatattaatt gtatttacct atttgag
//
LOCUS       DAELLV010000439          769 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.443, whole genome shotgun sequence.
ACCESSION   DAELLV010000439 DAELLV010000000
VERSION     DAELLV010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.443"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..592)
                     /locus_tag="KPD58_003739"
     CDS             complement(<1..592)
                     /locus_tag="KPD58_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0267417.1"
                     /translation="MYRIVICEDDITQIAFLRECILKSLEGISSQIELFEFNSGEELL
                     ETNLEGIDIFFLDIKMLQLTGMDVAKIIRETNDTSEIIFITSIVDYIQEGYKVRAYRY
                     LLKPIDFGDLNESILSCISDIIKKRENFMLIENKGIINKILINSIMYIEVRKKVLTIH
                     TKNDTYYTKNSMDKIELELEKYNFFRCHKSYLINLEY"
BASE COUNT          289 a          130 c           50 g          300 t
ORIGIN      
        1 tatactccaa attaattaaa tagctcttat gacatctaaa aaaattatat ttttctagct
       61 ctaattctat tttatccata ctatttttag tataataagt atcattttta gtatgtatag
      121 tcaaaacctt tttcctaacc tctatataca taatactatt aattaaaatt ttattgatta
      181 tacctttatt ttctatcagc ataaaatttt ctcttttttt tataatatcc gatatacaac
      241 tcaaaatact ttcatttaaa tctccaaaat ctataggctt taataaatat ctataagccc
      301 ttaccttata gccttcctgt atgtaatcaa ctattgaagt tataaatatt atttcagaag
      361 tatcattagt ttctctaatt attttagcca catccatgcc tgttagttga agcattttaa
      421 tatctaaaaa aaatatatca ataccttcta aatttgtttc caataattct tctcctgaat
      481 taaactcaaa tagttcaatt tgactagaaa taccctctaa ggactttaat atacactctc
      541 ttaaaaaagc tatttgtgta atatcatcct cacagataac tattctatac ataaacaccc
      601 ctccttagac attagatctg aaaacaaata aaatatcaat ttataaataa ttatttagtt
      661 atacaactaa catggtcatc aaatactatt attcacttaa aaaatcaccc cctattttta
      721 gtatgttaac aacaaaataa gaagatatct tagattttta aaaacaatt
//
LOCUS       DAELLV010000440          768 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.444, whole genome shotgun sequence.
ACCESSION   DAELLV010000440 DAELLV010000000
VERSION     DAELLV010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.444"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..280
                     /locus_tag="KPD58_003740"
     CDS             <1..280
                     /locus_tag="KPD58_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG0267418.1"
                     /translation="ACIKIKDTSIQKKIKLRGTVINRLFVIKCENTKINEVVLKNNKV
                     ITSKKDSFLHGIGISSVKSSVEKYNGNVEIYSDKNKFTITIYIPLTRN"
BASE COUNT          335 a           58 c          123 g          252 t
ORIGIN      
        1 agcttgtatt aagattaagg acacaagtat acaaaaaaag attaaattaa gaggaactgt
       61 tataaatagg ttatttgtaa taaaatgtga aaatacaaag ataaatgaag ttgtattaaa
      121 aaataataag gttattacaa gtaagaaaga ttccttttta catggaatag gaataagtag
      181 tgtaaaaagt tcagtagaaa aatataatgg aaatgtggaa atatactcag ataaaaacaa
      241 gtttacaatt actatttata taccattaac taggaactaa ttctagttgg ggctctaaat
      301 tgcccagttg gagcagtagt attgaaagat aaaatagaag atgctatctt taaggcatag
      361 gatatttgga ggtgataaaa atggttgaat gctatgctaa taagataaca tcctttttag
      421 tactcaataa aataatagaa caaaaaatgt gatttatatt tgtttgaatt taaaatgttg
      481 ataggtttta tagttatatt atttgcatga tatatcttaa ataaatttag aaaaatagag
      541 ttatttttat ctgcaaatgc gaccttagta tggtaacatt attgaccaaa agttctatat
      601 agtataaaaa aataaaatgt aaatttgata aactttataa ctaggaacat aaaaacaaca
      661 attaaataac taattagata gtaaagttga tattagtaat aaatattaaa gaaaaatatt
      721 ttactaattt gaggaggact tagttgtgat agattctgaa gaaatgat
//
LOCUS       DAELLV010000441          767 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.445, whole genome shotgun sequence.
ACCESSION   DAELLV010000441 DAELLV010000000
VERSION     DAELLV010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.445"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(249..473)
                     /gene="secG"
                     /locus_tag="KPD58_003741"
     CDS             complement(249..473)
                     /gene="secG"
                     /locus_tag="KPD58_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBG0267419.1"
                     /translation="MSNILMGVQVVLGILLVISIMPQDSKNALPSEFGGEGSQAYFKP
                     KGKQAFLARVTKITAVLFFINALALLIVKK"
BASE COUNT          306 a          116 c           65 g          280 t
ORIGIN      
        1 tagtgttgct atatattttt taatttgaaa aaaatatata aatacaaaca atatataaat
       61 ataaataata aacgccaaca aatagtcaat ctataaaata aaaactggac taatcatata
      121 aaatcattag tccagctttt attttacgta ctttttatgt tgatgtactt ttattaaaaa
      181 ctaaatttaa tttttaaatg tctagcaaat gcactaatta aataatggcc agttattcaa
      241 caatatcact acttcttaac tataagtaat gccaatgcat ttataaaaaa taatactgct
      301 gttatctttg taactctagc aagaaatgct tgttttcctt ttggtttaaa gtaagcttgg
      361 cttccttctc caccaaattc tgaaggtaaa gcattcttag aatcttgagg cattatgcta
      421 attactagta aaatccctag tactacttgc acacccatta aaatgttgct cattcttgcc
      481 cctcctatct gtttattact taaatttcat tactctttag tgtatcacaa taatatagcc
      541 atttcaagac tattatgcta taaataacat acaaaaaaat atattgttac tataatatat
      601 tactatattc tcaatataaa aatgaataaa acttagaaga gaaaactatt taaattaata
      661 cataaattaa cacttaaaat aatctactct cattgtatat attataaata gccaccaaaa
      721 taaaacaatc attttaaaat attgatataa cttttaattt caacata
//
LOCUS       DAELLV010000442          762 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.446, whole genome shotgun sequence.
ACCESSION   DAELLV010000442 DAELLV010000000
VERSION     DAELLV010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.446"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
BASE COUNT          254 a           87 c           80 g          341 t
ORIGIN      
        1 agggtataat ttttaaaatt atattctact atcaaaagat atgaataaat gattgtatat
       61 attatatcaa tttttatatt tcatagtcca ttttttcatc acattttcgc ttgtcaagaa
      121 taatatcttt aagagtgaag cgtttgatgt aaatctggac tttgtttttg agacattttt
      181 gaaatattta ttaaatcttt cgattatact tgtaatatat attatagata ttacttattt
      241 tagtaaaact aaaggatgat gctacaacat tccagttatt tcccaaaatt ttaatttctg
      301 aagtatactg tttacattat ttttaatcta ttttatctaa agtcgataag gcttcttatt
      361 cattaactga cctatataca gatttaaaat tacttattaa ttcttttgag tccttgtatg
      421 aaacatattt aaagtatttc ttatctaatg tattaaacat ttttgtatta cagaatgagg
      481 ataacttgta gctattgtct ctttgaaata agtcatctgt gtttactcct aaaactactt
      541 atatctgtag attttataag ttctccatct tatataattt ctttgattaa ttgttttctt
      601 aaattattca taaaaaaatc tccgttacaa tatttgatta tatttaaatc attgctataa
      661 agaagacttt tattaacttt gaaaaacaaa ttattttaat tgctggtgat tagttgctac
      721 cttcttttga tattaattct attagatttt atatagttat at
//
LOCUS       DAELLV010000443          753 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.447, whole genome shotgun sequence.
ACCESSION   DAELLV010000443 DAELLV010000000
VERSION     DAELLV010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.447"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..>753
                     /locus_tag="KPD58_003742"
     rRNA            <1..>753
                     /locus_tag="KPD58_003742"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          207 a          156 c          227 g          163 t
ORIGIN      
        1 tttaactgag cgttcagtta cttgacgtag acccgaaacc gggcgaccta cccatgagca
       61 ggatgaagcg aaagtaaaat ttcgtggagg tccgaaccca cgagcgttga aaagctcggg
      121 gatgacttgt gggtagcggt gaaattccaa tcgagcccgg agatagctgg ttctccccga
      181 aatagcttta gggctagcct caaggtgaga gatacggagg tagagcactg aatgtcctag
      241 ggggtattgc acctaccgaa gactatcaaa ctccgaatgc cgtcatctta tacttgggag
      301 tcagactgtg ggtgataaga ttcatagtcg aaagggcaac agcccagatc gtcagctaag
      361 gtccctaaat gtaagttaag tggtaaagga tgtgggattg cacagacaac caggatgttg
      421 gcttagaagc agccactcat tcaaagagtg cgtaatagct cactggtcga gtgatcctgc
      481 gccgaagatt tccggggcta aaacttacta ccgaagctac ggcatcagta atgatgggta
      541 ggggagcttc ccatacgggt tgaagcatga ccgtaaggac atgtggacag tatgggagtg
      601 agaatgttgg catgagtagc gagatgtggg tgagaatccc acaggccgta aacccaaggt
      661 ttccagggga aggttcgtcc gccctgggtt agtcgggacc taagctgagg ccgaaaggcg
      721 taggtgatgg acaacaggtt gatattcctg tac
//
LOCUS       DAELLV010000444          751 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.448, whole genome shotgun sequence.
ACCESSION   DAELLV010000444 DAELLV010000000
VERSION     DAELLV010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.448"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(66..674)
                     /locus_tag="KPD58_003743"
     CDS             complement(66..674)
                     /locus_tag="KPD58_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBG0267420.1"
                     /translation="MVDNLIKYIHKSDVAIVVLHEIYGINSFIEDVCQKYYKYGYDVF
                     CPELLGEKRVFPYSKVQEAYDFFINNVGFDIYKKVEKLINDLKSEYKYVFVAGYSVGA
                     TIAWRCSEDSFCDGIICCYGSRIRDYMDVIPKCPTLLVFAKHDSFDVDSISCQLSRKQ
                     NIQIEILDANHGFIDRYSNNYSDIQTKVFTIKEKSFLSKYLA"
BASE COUNT          280 a          131 c           68 g          272 t
ORIGIN      
        1 tcaatagtgt cataaaaact tttcagatta attaaatctt tctatatatt ttataatagt
       61 ctaaatcatg ctaaatattt agacagaaaa cttttttctt taatagtaaa tacttttgtt
      121 tgaatatcag aataattatt tgaatatcta tctataaaac catgatttgc atctaaaatc
      181 tcaatttgga tattttgttt cctagaaagt tggcaagaaa tagaatcaac atcaaatgaa
      241 tcgtgctttg caaatactaa taaggtagga cattttggaa ttacatccat gtaatctcga
      301 atccgagagc cataacaaca aattatgcca tcacaaaaag aatcttcact gcatctccat
      361 gcaatagttg ctcccacgct atacccagca acaaatacat atttatactc tgattttaaa
      421 tcatttataa gcttttcaac ttttttataa atatcaaaac caacattatt tataaaaaaa
      481 tcatatgcct cttggacttt tgaatatgga aaaactcttt tttctcccag aagttcagga
      541 caaaatacat catatccata tttataatac ttttgacaaa catcttctat aaagctatta
      601 atcccataga tttcatgcaa aacaacaata gctacatcac ttttatgtat atactttatc
      661 agattatcca ccacctttac aaaacagcaa attttttata cttagtttaa tatattcttc
      721 tttacatgtc aatttacttt tctaaaatca a
//
LOCUS       DAELLV010000445          716 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.449, whole genome shotgun sequence.
ACCESSION   DAELLV010000445 DAELLV010000000
VERSION     DAELLV010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.449"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..634)
                     /locus_tag="KPD58_003744"
     rRNA            complement(<1..634)
                     /locus_tag="KPD58_003744"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          173 a          182 c          127 g          234 t
ORIGIN      
        1 ctacttaacg taactcgttg gcccgttcta caaaaagtac gcggtcacac aagaaatgtg
       61 cttccacagc ttgtaagtgc agggtttcag gttctatttc actcccctcc cggggttctt
      121 ttcacctttc cctcacggta ctatacgcta tcggtcacta ggtagtattt agccttggag
      181 ggtggtccct cctgcttccc acagggtttc acgtgtcccg tggtactctg gatcatatct
      241 aaagtcttct cgtttaacat acaggactat taccttctgt ggtgggtctt tccaaacctc
      301 ttcaattacg atacctcttc gttatgatat gtccgcaacc ccaataaaga aaactttatt
      361 ggtttgggct aatccgcgtt cgctcgccgc tacttacgga atcgaatttc tttctcttcc
      421 ttcaggtact tagatgtttc agttccctga gttcccctca ttaagctatg tattcactta
      481 atgatactta gacattactc taagtgagtt tcctcattcg gaaatcttcg gatcaaagtt
      541 tacgtgcaac tccccgaagc ttatcgcagc ttatcacgtc cttcatcggc tcctagtgcc
      601 aaggcatccg ccctgcaccc ttaataactt gaccagttaa aaaggtttga tagattagaa
      661 gctatcaact tcgataattt taaaaagtta tcagctttat atattacaaa ttagat
//
LOCUS       DAELLV010000446          704 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.450, whole genome shotgun sequence.
ACCESSION   DAELLV010000446 DAELLV010000000
VERSION     DAELLV010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.450"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            162..530
                     /locus_tag="KPD58_003745"
     CDS             162..530
                     /locus_tag="KPD58_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0267421.1"
                     /translation="MKKIPKSELLVMNFIWDKGIDKIPAREVANYMEISHGWTKGTTG
                     KVLSRLSDKGFLEFEKDGRNTLYTIKVKHEEYLKFETKDFLELIHKNSIHSFISSLGC
                     GNEISDDDIKELEQWLKKRK"
BASE COUNT          283 a           72 c           95 g          254 t
ORIGIN      
        1 tcccctcttc cataaaactc tcaatatctt ttttatctat aaatctatac tacttagttc
       61 tcaagcataa aataatataa agattattta tacatgatga tatattatat tgactattag
      121 acatttgtct aatataatca agttaaaggt gagtgattat attgaaaaaa ataccaaaat
      181 ccgagttgtt agttatgaat ttcatatggg ataaaggcat agataaaata ccagccagag
      241 aagtagctaa ttatatggaa atttctcatg gatggacaaa aggtactact ggtaaagtac
      301 tttctagatt aagtgataaa ggttttttag aatttgaaaa agatggtaga aatactttat
      361 acactattaa agttaaacat gaggaatatc taaaatttga aacaaaggat tttttagagc
      421 ttatacataa aaattctatt catagcttta tatcatcatt aggttgtgga aatgaaatat
      481 cagatgatga cataaaagaa cttgaacaat ggctaaaaaa aagaaaatag gagacgtatt
      541 ttatggaatt atttatagaa gttattaaat ttaatattta cataagtcca ctaattcttt
      601 ttttcatttt atttactaaa aagataagca aatatacttt taagtttaat tattttatat
      661 gtcttttaat agtagtcaga atgatgttta tatctaaaat aaat
//
LOCUS       DAELLV010000447          687 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.451, whole genome shotgun sequence.
ACCESSION   DAELLV010000447 DAELLV010000000
VERSION     DAELLV010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.451"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            8..>687
                     /locus_tag="KPD58_003746"
     CDS             8..>687
                     /locus_tag="KPD58_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0267422.1"
                     /translation="MMKIAVITTEFLKEFVDNSIKKLNINAEIEIYIYRDFSHVGDLY
                     LEIEDRFDGFAVSGPIPKKAITKKAGTIKKPLVDFGTDLQSYYDMFLKLIFKYNTLDF
                     SRAYFDLTDWADNPKDISYYLSNGTFGDLMDSIDENASKMSLSDIAKLEKKIAKKHIN
                     LWNEGKIDFSTTRFSSIVPMLQNAGVNFHFIYPDIDLLKNTFNNLIKDIHLHRMKGNQ
                     PSVIYITV"
BASE COUNT          274 a           80 c          102 g          231 t
ORIGIN      
        1 gtaatctatg atgaaaatag cagtcataac aaccgaattt ttaaaagagt ttgtagataa
       61 ttctataaaa aagttaaata ttaatgcaga aattgaaata tatatctata gagatttttc
      121 tcatgttgga gatttatatt tagaaataga agataggttt gatggatttg cagttagtgg
      181 ccctatcccc aaaaaagcta ttacaaaaaa ggctggaact attaaaaaac ctctagttga
      241 ttttggtact gacttacaaa gttactatga tatgtttcta aagcttatct ttaaatataa
      301 tacattagat ttttctagag catactttga cttaacagac tgggcagata atccaaaaga
      361 tatatcatat tatttatcaa atggcacatt tggagactta atggatagta ttgatgaaaa
      421 tgcatctaaa atgagtttat ctgacatagc aaaacttgaa aaaaagattg caaaaaaaca
      481 tattaatcta tggaatgaag ggaaaatcga tttttctact actcgtttta gtagtattgt
      541 tcctatgtta caaaatgcag gagtaaactt tcatttcata taccctgata tagatttatt
      601 aaaaaatact tttaataact taataaaaga tattcattta catagaatga agggaaatca
      661 accaagtgta atatatataa cagttaa
//
LOCUS       DAELLV010000448          676 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.452, whole genome shotgun sequence.
ACCESSION   DAELLV010000448 DAELLV010000000
VERSION     DAELLV010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..676
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.452"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..>676
                     /locus_tag="KPD58_003747"
     CDS             <1..>676
                     /locus_tag="KPD58_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0267423.1"
                     /translation="FNINIKLFICIFITYVLYKINYVVDKWKSVFISLLYWMLLIGFD
                     SIGLSIVMTLNSIKNMNDLLEDNLLKLELIMISKSLLIVLIPLLKFIRLKTKINKRDC
                     IYLSIPLIANIIGVIVIFEFIFKDKIIDSKESLIILTISTVFLLSNLSLVSIIGRIIK
                     DNSLKIEYEITKKRMNMQYKYYLNLQEYQLKIRKLYHDMNNHIICIQNVYGKNEFADK
                     YIKDIN"
BASE COUNT          293 a           40 c           91 g          252 t
ORIGIN      
        1 agtttaatat aaatattaaa ttatttatat gtatatttat tacatatgta ttgtataaaa
       61 taaattatgt agtggataaa tggaaaagtg tatttataag tttgctatat tggatgttat
      121 taattggatt tgatagtatt ggattaagta tagtaatgac acttaattct ataaaaaata
      181 tgaatgattt attagaggat aatttgttga agttagagtt aataatgata tctaaatcat
      241 tactaatagt attaatacca ttgcttaagt ttatcagatt aaaaacaaaa ataaacaaga
      301 gggattgtat atatttatct attccattaa tagcaaacat tattggtgta atagtaatat
      361 ttgaatttat ttttaaagat aagattatcg attctaaaga gagtttaata atacttacaa
      421 tatcaactgt atttttacta tcaaatcttt cattagtaag cataatagga agaataataa
      481 aagataatag tttaaagata gaatatgaaa ttacaaaaaa aagaatgaat atgcaatata
      541 aatattattt aaatttacag gaatatcagt tgaaaataag aaaactatat catgatatga
      601 ataatcatat catttgtatt cagaatgttt atgggaaaaa tgagtttgca gataagtata
      661 ttaaagatat aaataa
//
LOCUS       DAELLV010000449          674 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.453, whole genome shotgun sequence.
ACCESSION   DAELLV010000449 DAELLV010000000
VERSION     DAELLV010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..674
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.453"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            208..>674
                     /locus_tag="KPD58_003748"
     CDS             208..>674
                     /locus_tag="KPD58_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0267424.1"
                     /translation="MKKSVALVNDSRKDLIDFLENNLKLVFGDSININRYFINEINDN
                     DIINDDVILVMSVERLDKIINNILDKKKVIVVRRTFREDKIYNLLSLPQGTNVLIVND
                     SDETTLETISLFYKIGVTNIRPIPYMNDNNYKNIKIAITPGVPEKVPSFISDI"
BASE COUNT          285 a           59 c           95 g          235 t
ORIGIN      
        1 aaatatacat acagtttaag ttttaagcaa caattagggg gatttggtga caagattttt
       61 aattataaga aagaaagtgt cttaatgcaa cttttataaa gtcattatag acactttctt
      121 ttttatttga taaatttaaa ttatcaaaat tatattgctt tatacaacta ttttgttgga
      181 aactatttat tatataagga gaaataaatg aaaaaatcag tagctctggt aaatgatagt
      241 agaaaagatt taatagactt tttagaaaat aatttgaaat tagtttttgg agattctata
      301 aatattaata gatacttcat aaatgaaata aatgataatg atattataaa tgatgatgtg
      361 atattagtaa tgtcagtaga aagattagac aaaattataa ataatatact agataagaaa
      421 aaagtgattg tggtaagaag aacttttagg gaagataaaa tttataatct actatcttta
      481 ccacagggta caaatgttct aatcgtaaat gattctgatg aaactactct agaaactata
      541 agtttatttt ataaaattgg agtgactaat attagaccaa ttccatatat gaatgataat
      601 aactataaaa atataaaaat agctataact ccaggagttc cagaaaaagt acctagcttt
      661 atctcagata tatt
//
LOCUS       DAELLV010000450          670 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.454, whole genome shotgun sequence.
ACCESSION   DAELLV010000450 DAELLV010000000
VERSION     DAELLV010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.454"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            38..>670
                     /locus_tag="KPD58_003749"
     CDS             38..>670
                     /locus_tag="KPD58_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBG0267425.1"
                     /translation="MNHHILIQFEYIARIITAGFCGALIGYERKNRLKEAGIRTHFIV
                     ALASALIIIVSKYGFNDIVGTPGIGLDPARVAAQVVSGIGFLGAGLIFIRNQSVSGLT
                     TAAGIWATAGTGLAIGSGLYLVGIVSSIIIVIVQITLHRDRKWMKLPTAEQVSLKISN
                     STDSIEYIEGKFKKSNIEIINMNIKRLDGDWLEVVIYTKLPKGYKKTNLLK"
BASE COUNT          229 a           88 c          133 g          220 t
ORIGIN      
        1 gtatatataa tattatatag aagggggtgc tagataaatg aaccatcata tattgattca
       61 atttgaatat atagcaagaa tcattactgc tggtttctgt ggggctttaa ttggatatga
      121 gagaaagaat agactaaaag aagctggaat acgcactcat tttatagtgg cattagcttc
      181 agcacttata atcattgttt ccaagtatgg atttaatgat atagtgggta ctccaggtat
      241 tggtctagac ccagcccgcg ttgcagcaca agttgtcagt ggtataggtt ttttaggtgc
      301 tggattaata tttattagaa atcaatcagt aagtggtctt actacagctg ctggtatatg
      361 ggcaacagct ggtacaggtc ttgccattgg ctctggatta tacttagttg gtattgtttc
      421 aagtatcatt attgttattg tacagataac acttcataga gataggaaat ggatgaaact
      481 ccctacagca gagcaagttt ctcttaaaat ttctaacagc actgattcca tagaatatat
      541 tgaaggtaaa tttaaaaaat ctaatattga aattataaac atgaatatta aacgccttga
      601 tggggattgg ttagaagtag ttatatatac taagttacct aaaggatata aaaaaacaaa
      661 tttattaaaa
//
LOCUS       DAELLV010000451          670 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.455, whole genome shotgun sequence.
ACCESSION   DAELLV010000451 DAELLV010000000
VERSION     DAELLV010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.455"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            124..>670
                     /locus_tag="KPD58_003750"
     CDS             124..>670
                     /locus_tag="KPD58_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0267426.1"
                     /translation="MFKKIVAMASSIIVSTICLTGCDFNSNDKKDESSSSKNLDKVTI
                     AEVTHSVFYAPQYAAITKGFFEDEGIKIDLINTQGADKTMAALISGEANVGLMGPEAS
                     IYVYNQNKNDYAVNFAQLTKRDGSFLVARKKMPNFSYNDLKGTEILGGRKGGVPLMTF
                     EYVLKEKGLTIGENLKAGNVNV"
BASE COUNT          251 a           84 c          117 g          218 t
ORIGIN      
        1 taaaactgaa aattattatt aagtttttgt agaaatatta taagcataga tacatttttg
       61 taacaactac aataaatttt taatactata tactattcgt atgtcttatg ggaggtattt
      121 attatgttta aaaaaatagt cgcaatggca agctctatca ttgtaagtac aatatgtcta
      181 actggatgtg attttaactc taatgacaaa aaagatgaat cttcttcgtc taaaaatctg
      241 gataaagtaa ctatagcaga agttactcac tctgtttttt atgctccaca gtatgctgct
      301 attacgaaag gattctttga agatgaaggt ataaagattg accttataaa tacccaaggt
      361 gcagataaaa ctatggcagc tttgatttca ggtgaagcaa acgtaggttt aatgggtcct
      421 gaagcatcta tatatgtata taatcaaaac aaaaacgatt atgcagtgaa ctttgctcaa
      481 ttgacaaaaa gagatggtag ttttctagtt gctaggaaaa aaatgcctaa tttctcttac
      541 aatgatttaa aaggaaccga aatattaggt ggtagaaaag gtggagttcc actaatgact
      601 tttgaatatg ttctaaaaga aaaaggtctt actattggag aaaatctaaa agcaggcaat
      661 gttaatgtaa
//
LOCUS       DAELLV010000452          669 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.456, whole genome shotgun sequence.
ACCESSION   DAELLV010000452 DAELLV010000000
VERSION     DAELLV010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.456"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..353
                     /gene="srtB"
                     /locus_tag="KPD58_003751"
     CDS             <1..353
                     /gene="srtB"
                     /locus_tag="KPD58_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sortase SrtB"
                     /protein_id="HBG0267427.1"
                     /translation="NIIIYGHNMKNKTMFNNLNKFKDADFFKKNNKIKITLNGKEFLY
                     DVFSAYIVESDYDYLKTNFNNESDYQNYINDITSKSLYKSPIKVNSNDKIVTLSTCTY
                     EFDDARMVIHGRLI"
     gene            complement(513..587)
                     /locus_tag="KPD58_003752"
     tRNA            complement(513..587)
                     /locus_tag="KPD58_003752"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(553..555),aa:Thr,seq:tgt)
BASE COUNT          287 a           95 c           74 g          213 t
ORIGIN      
        1 aaaatatcat aatatatgga cataatatga aaaataaaac tatgttcaac aacctaaata
       61 agtttaaaga tgctgacttc tttaaaaaaa ataataaaat aaaaataacc ttgaatggaa
      121 aagaattttt atatgatgtt ttttctgctt atattgtaga atctgattat gattatctaa
      181 aaacaaattt taacaatgaa tctgattatc aaaattacat aaatgatatt acttctaaat
      241 cattgtataa atctcctatt aaggtaaata gcaatgataa aatagttaca ctttctacat
      301 gtacctatga atttgatgat gccagaatgg tgattcatgg tagattgatt taaaactcac
      361 agacacagtt taaaacaata attttaaact gtgtcatctt ttatacctat aatttgtaaa
      421 ttagaccact aaaagtattt aatacaaaat aacaaataca tcaaacaata aaaaacaata
      481 aaaaaagact atgctaaaaa catagtcttt actggagctg gtgataggaa tcgaacctac
      541 aacctgctga ttacaagtca gctgctctac cgttgagcca caccagcaca cctattttat
      601 tgtaaattag caaaactaat ttttaaaagt taacactcta aaaactacac atatatctta
      661 atgaatgaa
//
LOCUS       DAELLV010000453          664 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.457, whole genome shotgun sequence.
ACCESSION   DAELLV010000453 DAELLV010000000
VERSION     DAELLV010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.457"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..>664)
                     /locus_tag="KPD58_003753"
     rRNA            complement(<1..>664)
                     /locus_tag="KPD58_003753"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          152 a          191 c          151 g          170 t
ORIGIN      
        1 gatccagccg caccttccga tacggctacc ttgttacgac ttcaccccag ttattgattc
       61 caccttcgac gacttcttcc aaaaggttag ataatcggct tcgggcgtct ccaactcccg
      121 tggtgtgacg ggcggtgtgt acaagacccg ggaacgcatt caccgcagca ttctgatctg
      181 cgattactag taactccagc ttcatgtagg cgagtttcag cctacaatcc gaactgagag
      241 tagctttaag ggattagctc caccttacgg cttggcaacc ctctgtacta cccattgtag
      301 cacgtgtgta gccctaagca taaggggcat gatgatttga cgtcatcccc accttcctcc
      361 aggttatccc tggcagtctc tctagagtgc ccaacttaat gatggcaact aaagacaagg
      421 gttgcgctcg ttgcgggact taacccaaca tctcacgaca cgagctgacg acaaccatgc
      481 accacctgtc accaatgtcc ccgaagggaa ctctccgatt aaggagatgt cattgggatg
      541 tcaagcttag gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc cgctacttgt
      601 gcgggtcccc gtcaattcct ttgagtttca ctcttgcgag cgtacttccc aggcggagta
      661 ctta
//
LOCUS       DAELLV010000454          587 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.459, whole genome shotgun sequence.
ACCESSION   DAELLV010000454 DAELLV010000000
VERSION     DAELLV010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.459"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..584
                     /locus_tag="KPD58_003754"
     CDS             <1..584
                     /locus_tag="KPD58_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG0267428.1"
                     /translation="NREGRSIKQFRNKMYAYISKRLSKCKKGSRQSKKLWRLKNKIRS
                     KTDNQLMNLYHQTTRKFIDFCVEQKVCEIVLGDIKGVEKDTKKKKRLNRVNRQKISQM
                     EYGRIKDYIKYKAKEQGIEVKLVKENYTSQTCPKCSKKHKPTGRTYSCTCGYETHRDI
                     VGAWNILNKKHKYGLVDFRINHKQPINLKVSTV"
BASE COUNT          265 a           55 c          107 g          160 t
ORIGIN      
        1 ctaatagaga aggcagaagt attaaacaat ttagaaataa gatgtatgct tatatatcta
       61 aaagattaag taaatgtaaa aaaggtagta gacaatcaaa gaagttatgg agattaaaaa
      121 ataagataag aagtaaaaca gataatcagt taatgaattt atatcatcaa acaactagaa
      181 agtttataga cttttgtgta gagcagaagg tatgtgaaat agttcttggg gatataaaag
      241 gggttgaaaa agatactaag aaaaagaaaa gattaaacag agttaataga cagaaaatat
      301 ctcaaatgga gtatggtcga ataaaggact atataaaata taaagcaaaa gagcaaggta
      361 ttgaagttaa gttagttaaa gaaaactaca cttctcaaac ttgcccaaag tgttctaaaa
      421 aacacaaacc aactggtaga acttatagtt gtacttgtgg ttatgaaact catagagata
      481 tagttggtgc ttggaatatt ttaaataaaa agcataaata tggtttagta gattttagaa
      541 taaatcataa gcaaccaata aatttaaaag tatctaccgt ttgagcc
//
LOCUS       DAELLV010000455          586 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.460, whole genome shotgun sequence.
ACCESSION   DAELLV010000455 DAELLV010000000
VERSION     DAELLV010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.460"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..>586
                     /locus_tag="KPD58_003755"
     CDS             <1..>586
                     /locus_tag="KPD58_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBG0267429.1"
                     /translation="SSIIEIWSCKKVFDYTSQSKTDLKKLNMYFLFSVFLIISSLYIN
                     IGANSRILICIFVMFVFYKSNYKVNIYKCLVISFFYWLILIGINGLSMLLVATVSNLD
                     NMNVLLSGSLYRYEAIFLGKIMLGILLYVYKASFKNFKISKKEIYICIPMIANLLSFF
                     VLYDYIINLVKISPERRTAFLVIVLLLILSNISIF"
BASE COUNT          203 a           54 c           77 g          252 t
ORIGIN      
        1 ttcttctatt atagaaatat ggtcatgtaa aaaagttttt gattatacta gtcagtcaaa
       61 gaccgactta aaaaaactaa atatgtattt tctcttttct gtttttctca ttatttcttc
      121 tttatatata aatataggag ctaatagtag aatcttgata tgtatatttg taatgttcgt
      181 tttctataag tctaactata aggttaatat atataaatgt ctggtaatct cattttttta
      241 ttggttaata ttaataggta taaatggatt aagtatgtta ttagtagcaa cagtaagcaa
      301 tttggataat atgaatgttt tacttagtgg cagtttatat agatatgaag caatattctt
      361 gggaaagata atgttaggta tattacttta tgtttataag gcttctttta aaaattttaa
      421 aataagtaaa aaagagatat acatatgtat tcctatgata gcaaatttat tgtcattttt
      481 tgtactttat gattatatta ttaatcttgt aaaaatctct cctgaaagaa gaactgcttt
      541 tttagtaata gttttactat taatattatc taacatttca atcttt
//
LOCUS       DAELLV010000456          577 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.461, whole genome shotgun sequence.
ACCESSION   DAELLV010000456 DAELLV010000000
VERSION     DAELLV010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.461"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..>577
                     /locus_tag="KPD58_003756"
     rRNA            <1..>577
                     /locus_tag="KPD58_003756"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          154 a          121 c          190 g          112 t
ORIGIN      
        1 caggatggac ccgcgtctga ttagctagtt ggtaaggtaa cggcttacca aggcgacgat
       61 cagtagccga cctgagaggg tgatcggcca cattggaact gagacacggt ccaaactcct
      121 acgggaggca gcagtgggga atattgcaca atgggcgaaa gcctgatgca gcaacgccgc
      181 gtgagtgatg aaggccttcg ggtcgtaaaa ctctgtcctc aaggaagata atgacggtac
      241 ttgaggagga agccccggct aactacgtgc cagcagccgc ggtaatacgt agggggctag
      301 cgttatccgg atttactggg cgtaaagggt gcgtaggcgg tctttcaagt caggagtgaa
      361 aggctacggc tcaaccgtag taagctcttg aaactgggag acttgagtgc aggagaggag
      421 agtggaattc ctagtgtagc ggtgaaatgc gtagatatta ggaggaacac cagttgcgaa
      481 ggcggctctc tggactgtaa ctgacgctga ggcacgaaag cgtggggagc aaacaggatt
      541 agataccctg gtagtccacg ctgtaaacga tgagtac
//
LOCUS       DAELLV010000457          573 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.462, whole genome shotgun sequence.
ACCESSION   DAELLV010000457 DAELLV010000000
VERSION     DAELLV010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.462"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..>573
                     /gene="tuf"
                     /locus_tag="KPD58_003757"
     CDS             <1..>573
                     /gene="tuf"
                     /locus_tag="KPD58_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBG0267430.1"
                     /translation="IPAPERDTDKPFLMPVEDVFSITGRGTVATGRVERGVLKVQDEV
                     ELVGLTEAPRKVVVTGVEMFRKLLDQAQAGDNIGALLRGVQRNEIERGQVLAKTGSVK
                     AHTKFTAEVYVLKKEEGGRHTPFFDGYRPQFYFRTTDVTGACKLPEGIEMVMPGDNVT
                     MEVDLINSIVVEEGLRFSIREGGRTVASGV"
BASE COUNT          242 a           77 c          140 g          114 t
ORIGIN      
        1 atataccagc tccagagaga gatacagata aaccattctt aatgccagta gaggacgtat
       61 tctcaatcac aggaagagga acagttgcaa caggaagagt ggaaagagga gtactaaaag
      121 tacaagacga agtagaatta gtaggattaa cagaagcacc aagaaaagta gtagtaacag
      181 gagtagagat gttcagaaaa ttattagacc aagcacaagc aggtgataat ataggagcat
      241 tattaagagg agtacaaaga aacgagatag aaagaggaca agtactagca aagactggat
      301 cagtaaaggc acacacaaag tttacagcag aagtatatgt acttaaaaaa gaagagggtg
      361 gaagacatac accattcttt gatggatata gaccacaatt ctatttcaga acaacagacg
      421 taacaggagc ttgtaagtta ccagaaggaa tagagatggt aatgcctgga gataacgtaa
      481 caatggaagt agacttaata aactcaatag ttgtagaaga gggattaaga ttctcaataa
      541 gagaaggtgg aagaacagta gcttcaggag ttg
//
LOCUS       DAELLV010000458          551 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.464, whole genome shotgun sequence.
ACCESSION   DAELLV010000458 DAELLV010000000
VERSION     DAELLV010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.464"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..488
                     /locus_tag="KPD58_003758"
     CDS             <1..488
                     /locus_tag="KPD58_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0267431.1"
                     /translation="GVMAGAFAGGEGDFTTAFEPTATAMEKEGSGYIVTSIGKDSGEI
                     PFTAYSTTRSYMKNNKDLIQRFTNAVYKGQLWIQQASSKEVAKAMQPFFEDISLEDLT
                     TVVDRYKSIDAWSQNPVLKEEGLKKLMTVMKEAKELDKEAPYKDIVNTEFANNAIKNI
                     K"
BASE COUNT          225 a           66 c           99 g          161 t
ORIGIN      
        1 ttggtgtaat ggctggagct tttgcaggtg gagagggtga ctttacaaca gcatttgaac
       61 ctacagcaac agcaatggaa aaagaaggta gtggatatat agtcacatct ataggaaaag
      121 attctggaga aatacctttt acagcatact ctactacgag atcatacatg aaaaacaata
      181 aagatttaat tcaaagattt acaaatgctg tttataaagg tcaattatgg attcaacaag
      241 catctagcaa agaagtagct aaggctatgc aacctttctt tgaagatata tctttagaag
      301 atttaacaac agttgtagat agatataaat caatagatgc atggtctcaa aatcctgtat
      361 taaaagagga aggtttgaag aaattgatga ctgttatgaa agaagctaaa gaactagata
      421 aggaagcacc ttataaagat atcgtaaata ctgagtttgc aaacaatgct ataaaaaata
      481 ttaaataaaa tatattagtt ttagctagat attttcttgt ttttataatg aaaattaaca
      541 aaaatataaa a
//
LOCUS       DAELLV010000459          536 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.465, whole genome shotgun sequence.
ACCESSION   DAELLV010000459 DAELLV010000000
VERSION     DAELLV010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.465"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            198..>536
                     /locus_tag="KPD58_003759"
     CDS             198..>536
                     /locus_tag="KPD58_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0267432.1"
                     /translation="MYCLSVVLAIYGIYVFINCTEYIYTMLSTKHLTVKGNEYTIINY
                     YMSNFSQYIIFAILLASMGFVIQKIVHMHFSEIELSGNYSTTVRNISDISLDNDNEMF
                     NRGNIENSILK"
BASE COUNT          217 a           53 c           73 g          193 t
ORIGIN      
        1 gggtacaatg tatagcaaat ataaatattg gcttaataaa actctactct taagtctact
       61 gtcgttttaa cacaagacta ataataagtg taacctaaaa attattgata atacaataac
      121 cttgtttatg ataaataaca aatattaagt gttgtaaaga tagtaagttt aaaaaaatat
      181 caaagatttc aatcacaatg tattgtttat ctgtagtgtt agctatttat ggtatatatg
      241 tatttataaa ttgtactgaa tatatatata ctatgctatc tacaaaacat ttaacagtca
      301 aaggaaatga atataccatt ataaattatt atatgagtaa ctttagtcag tatataatat
      361 ttgctatttt acttgcatca atgggatttg taatacaaaa gatagtgcat atgcatttca
      421 gtgagataga acttagtggt aactattcaa caactgttag aaatataagt gatatctctt
      481 tagataatga taatgaaatg tttaatagag gcaatattga aaatagtatt ttaaaa
//
LOCUS       DAELLV010000460          528 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.467, whole genome shotgun sequence.
ACCESSION   DAELLV010000460 DAELLV010000000
VERSION     DAELLV010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.467"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(260..376)
                     /gene="rrf"
                     /locus_tag="KPD58_003760"
     rRNA            complement(260..376)
                     /gene="rrf"
                     /locus_tag="KPD58_003760"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(385..458)
                     /locus_tag="KPD58_003761"
     tRNA            complement(385..458)
                     /locus_tag="KPD58_003761"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Gly,seq:tcc)
BASE COUNT          206 a           89 c           64 g          169 t
ORIGIN      
        1 ttaacataaa tttctacttt gttttttaac cttttaattt ttgaaattat atcaaagtgt
       61 ttttacccaa aattataaat aagtacataa aaaataaact tctaaaaaaa ttataaaaac
      121 aaaaaataaa aaaattaaat atattgaata tatttaatta tcttcactat atcatttatt
      181 gccaaaaaaa tctataaaag acaaataaaa acatataaca attgtaggtt tattaaattt
      241 aatacaacaa aaaaagatta cgtggctacg tcctactctc ccaggcagtt tcccaccaag
      301 taccatcagc gctagagagc ttaacttctg tgttcggaat gggaacaggt gtatcctctc
      361 tgctattgta accacataat cttttggagc gggtgaaggg gatcgaaccc tcacagctgg
      421 cttggaaggc cagaactcta ccattgagct acacccgcat taaattttta taaattagta
      481 aaactaattt tttagagtta gcactctaaa aactacatat atatccta
//
LOCUS       DAELLV010000461          493 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.468, whole genome shotgun sequence.
ACCESSION   DAELLV010000461 DAELLV010000000
VERSION     DAELLV010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.468"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            188..304
                     /gene="rrf"
                     /locus_tag="KPD58_003762"
     rRNA            188..304
                     /gene="rrf"
                     /locus_tag="KPD58_003762"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            310..398
                     /locus_tag="KPD58_003763"
     tRNA            310..398
                     /locus_tag="KPD58_003763"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:344..346,aa:Leu,seq:taa)
     gene            410..486
                     /locus_tag="KPD58_003764"
     tRNA            410..486
                     /locus_tag="KPD58_003764"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:444..446,aa:Ile,seq:cat)
BASE COUNT          156 a           91 c          109 g          137 t
ORIGIN      
        1 attcattaag atatatgtgc agtttttaga gtgctaactc tatattttta aacttgtcaa
       61 aacttatgaa tttttaatag ttttataagg caaaaaatcg aataaaaaaa gtattgacac
      121 aataagacaa agatgttatt ataatacttg tcttagaaaa aaagttaaat gaaaatttag
      181 acaataatct ggttataata gcagagagga tacacctgtt cccattccga acacagaagt
      241 taagctctct agcgctgatg gtacttggtg ggaaactgcc tgggagagta ggacatagcc
      301 aggtgatgtg ccgaagtggc ggaactggca gacgcacagg acttaaaatc ctgcgggact
      361 tacctctcgt accggttcga ttccggtctt cggcaccaca aattattgtg ggccattagc
      421 tcagttggtt agagcgcccg gctcataacc ggtaggtctg gggttcgagt ccctgatggc
      481 ccaccacaat aat
//
LOCUS       DAELLV010000462          486 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.469, whole genome shotgun sequence.
ACCESSION   DAELLV010000462 DAELLV010000000
VERSION     DAELLV010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.469"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            39..155
                     /gene="rrf"
                     /locus_tag="KPD58_003765"
     rRNA            39..155
                     /gene="rrf"
                     /locus_tag="KPD58_003765"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            275..466
                     /locus_tag="KPD58_003766"
     CDS             275..466
                     /locus_tag="KPD58_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HBG0267433.1"
                     /translation="MKKVALILAIIGALNWGAIGILGTDLIGSIFGGTYEMVSRIIYF
                     IVGLAGLYLIPSLMSDDRE"
BASE COUNT          168 a           57 c           96 g          165 t
ORIGIN      
        1 tattaaaaac ttaatagaaa tattaagcat aaagattatg tggttacaat agcagagagg
       61 atacacctgt tcccattccg aacacagaag ttaagctctc tagcgctgat ggtacttggt
      121 gggaaactgc ctgggagagt aggacgtagc cacgtaattt tttttatttt ttgtcaaaaa
      181 aaacaaaaaa tacgtatttc ttttttcatt tcgggaaaac taaacacagg aattatggac
      241 tttataaaaa tttaattctt gaggagtgat ataagtgaaa aaagttgctt taattttagc
      301 tataataggt gcgcttaact ggggtgcaat tggtattcta ggtacagact taataggtag
      361 tatctttggt ggaacatatg aaatggtaag tagaataata tacttcattg taggtttagc
      421 tggattatac ttaataccaa gtttaatgtc tgatgataga gaataattta tgcttttatg
      481 ttaaat
//
LOCUS       DAELLV010000463          482 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.470, whole genome shotgun sequence.
ACCESSION   DAELLV010000463 DAELLV010000000
VERSION     DAELLV010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.470"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            100..447
                     /locus_tag="KPD58_003767"
     CDS             100..447
                     /locus_tag="KPD58_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBG0267434.1"
                     /translation="MHENKKNLLDGSLAQYNDTAVPKIPVFAGNDITSEVYYFKPNQV
                     LNAHRHPNGEQIFVFLKGEGKMKLGEHECDVKNGDTVFVPTGEWHEITNGSNEEMVAV
                     QITKINAGAEYRG"
BASE COUNT          192 a           56 c           87 g          147 t
ORIGIN      
        1 aataaaagaa ttttattcct attgtgtaaa gtgagaatac tattttctta tattggtata
       61 ataatagtaa atctaatata gaaaagaggt atttctagta tgcatgaaaa taaaaagaat
      121 ctattagacg gaagcttagc tcaatataat gatacagcag ttccaaaaat accagtattt
      181 gctggtaacg atataacttc agaagtttat tattttaaac ctaatcaagt tcttaatgct
      241 catagacacc ctaatggtga acaaatattt gtgttcctaa aaggagaagg taaaatgaaa
      301 cttggtgagc atgaatgtga tgtaaaaaac ggcgatactg tatttgtacc aacaggagag
      361 tggcatgaaa ttactaatgg aagcaatgaa gaaatggtag ccgttcaaat tacaaaaatt
      421 aatgctggag cagaatacag aggatagata tataagtaca gttagtactt taatatctat
      481 tt
//
LOCUS       DAELLV010000464          477 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.471, whole genome shotgun sequence.
ACCESSION   DAELLV010000464 DAELLV010000000
VERSION     DAELLV010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.471"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
BASE COUNT          191 a           28 c           65 g          193 t
ORIGIN      
        1 actgtgaaat ggatatataa ctttaggtat tgtgtattat tttcactgta ggagattata
       61 tctagttata cataataagt tttaatgata gtttatagta agatattaaa ataaatttat
      121 ctaatctaga tatgaaataa gttattactt taatataaaa atgaatctaa tggaataata
      181 ttgatgataa gtaatattta gattttgtat ttgtttatag ttaataagtg gattatagaa
      241 tttataataa ttaatatata ttaaacaaag atatattaag atattttaga acataaaact
      301 gaattaaggc gcgagtacta ttttgtattc gcgccttgat ggtttgagtt atttgagtaa
      361 aaactaactt taataagaaa tatgtattta ttaagttgat atttttaatg tgtttagaga
      421 ttatttaaca atctccaaat ataaaactag aattatttac ataaaagtta aatagtt
//
LOCUS       DAELLV010000465          463 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.472, whole genome shotgun sequence.
ACCESSION   DAELLV010000465 DAELLV010000000
VERSION     DAELLV010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.472"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..260
                     /locus_tag="KPD58_003768"
     CDS             <1..260
                     /locus_tag="KPD58_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0267435.1"
                     /translation="SVYYFTKFFKKNTGKTFITFLNEYRLEKAKWILLNNNISIVDLV
                     EEVGFGSTKTFYRVFKEKIGVSPLEFKKRYNSLEKKGSVSK"
BASE COUNT          196 a           46 c           59 g          162 t
ORIGIN      
        1 ttagtgtata ttattttact aaatttttca aaaaaaatac aggaaaaaca tttataactt
       61 ttttaaatga atatagatta gaaaaagcaa agtggatatt acttaataac aatatatcca
      121 ttgtagattt ggttgaagaa gttggttttg gaagtacaaa aactttttac agagtattta
      181 aagaaaaaat tggagtctct ccattagaat ttaaaaaaag atataatagc ttggaaaaaa
      241 aagggagtgt ctcaaaatga acttagttca tgaggcactt tcttttatat gaaatcaaat
      301 atattttcag tatttcaaca atgaaaattc cattcattta taaagtctaa attttagttc
      361 tcaatacaac ctctatttcg aaataaatcc ttttatatat aaatatacag caatatatga
      421 gaataagata acaataatga ggatataaaa atgtatttgc tat
//
LOCUS       DAELLV010000466          451 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.473, whole genome shotgun sequence.
ACCESSION   DAELLV010000466 DAELLV010000000
VERSION     DAELLV010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.473"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
BASE COUNT          179 a           57 c           48 g          167 t
ORIGIN      
        1 cctttgaaaa tattttaaat ggttttttaa atttgatttt aaaaaatgat tttgatataa
       61 aaaaaccaca cagtatatat aactgtatgg caaaaattcg aataagccac acagtataat
      121 ccatgtggct ctatattatg atgataaaat aaaaacttta acaacaatta ctatagccaa
      181 catagataca atccttaaaa atcataatcc taaggggctt gtatctatgt tatgacaatt
      241 tgtcttaact taggtacaaa ccctaactaa attggctact aaaaaatcgt acttgtttgt
      301 ttaataaagt cttcatattt acctctcctt ttaatttaat aattttgaaa attttgtttt
      361 ctgttttgta tagtaattag tttaatacag taaaaaataa aagtcaacta taaattttga
      421 aaaattatca aattatcaga aaattgagtt t
//
LOCUS       DAELLV010000467          434 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.475, whole genome shotgun sequence.
ACCESSION   DAELLV010000467 DAELLV010000000
VERSION     DAELLV010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.475"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..408)
                     /locus_tag="KPD58_003769"
     CDS             complement(<1..408)
                     /locus_tag="KPD58_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufBD protein"
                     /protein_id="HBG0267436.1"
                     /translation="MKNLNLLINKLYSKNHNEAYKTFLFLENESLKSNITYCFFDSFL
                     EMIDNENSYIRTRGLLLISANAQWDIDNKIEINIDSILSHIVDKKPSVSRMFIKSIPN
                     ITKYKENLINRIKMELSNADISIYNNNMKPLVEK"
BASE COUNT          146 a           57 c           42 g          189 t
ORIGIN      
        1 cttttctact aatggtttca tattattatt ataaatactt atatctgcat tacttaattc
       61 catttttatg cgattaataa gattttcttt atactttgta atattaggta tggatttaat
      121 aaacattctt gatacagaag gttttttatc tacgatatga cttagtatac tatctatatt
      181 tatttcaatt ttattatcaa tatcccactg agcatttgca gagattaata acaatcccct
      241 tgttcgaata tatgagtttt cgttatcaat catttctagg aatgagtcaa aaaaacaata
      301 tgtaatattt gattttaaac tttcattttc taagaataaa aaagttttat atgcttcatt
      361 atgatttttt gaatataatt tattaataag taaattgaga ttcttcaatc ttctcatctc
      421 cctcattaca aata
//
LOCUS       DAELLV010000468          404 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.477, whole genome shotgun sequence.
ACCESSION   DAELLV010000468 DAELLV010000000
VERSION     DAELLV010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.477"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
BASE COUNT          128 a           60 c           28 g          188 t
ORIGIN      
        1 caatttaaat tttaaatatt ttgtgtttat tatataatct gaaaaaaaag cagtcaatag
       61 catttttaat ttttttaatt ttcaaaatta tgattctcaa aaaatcgttt tccttttatc
      121 ataacaccta tttacgttaa ttcttgtagt attttgtatt tattttgcta aattgacagt
      181 taactttatt caatttttat tctacttgcg tagatttacc tcttgttttt tactcttaaa
      241 accatttttt ctgaaaattc cttattatga tttatcaact tatttttctt cataacaatt
      301 actctctaac ttccaactta cttaaacttt gcagttgcta gtattcacgt ttacatactt
      361 atatgtcata attaactaaa aattcttaat atatctctca atga
//
LOCUS       DAELLV010000469          363 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.479, whole genome shotgun sequence.
ACCESSION   DAELLV010000469 DAELLV010000000
VERSION     DAELLV010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.479"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            167..283
                     /gene="rrf"
                     /locus_tag="KPD58_003770"
     rRNA            167..283
                     /gene="rrf"
                     /locus_tag="KPD58_003770"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            290..361
                     /locus_tag="KPD58_003771"
     tRNA            290..361
                     /locus_tag="KPD58_003771"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:322..324,aa:Asn,seq:gtt)
BASE COUNT          116 a           61 c           78 g          108 t
ORIGIN      
        1 ttcattaaga tatatgtgca gtttttagag tgttaactct atgtaaagta ctaacttcaa
       61 ttgatgaagt atatatcgta aaaacaatta ctatttagta cttcaaaact attgacattt
      121 aaaaataaag atgttaatat aatacttgtc ttataaatag gcaatatgtg gttataatag
      181 caaagaggat acacctgttc ccattccgaa cacagaagtt aagctcttta gcgctgatgg
      241 tacttggtgg gaaactgcct gggagagtag gacgtagcca cgtgaatatt ccaaggtagc
      301 tcaatggtgg agcaaccggc tgttaaccgg taggctgtgg gttcgagccc cacccttgga
      361 gcc
//
LOCUS       DAELLV010000470          333 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.480, whole genome shotgun sequence.
ACCESSION   DAELLV010000470 DAELLV010000000
VERSION     DAELLV010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.480"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
BASE COUNT          126 a           38 c           36 g          133 t
ORIGIN      
        1 ctgtatatgc tatcctttgc aatttaattt taaaatttaa tttatcatat attatataga
       61 atataagtat tacacatatt atagccataa taagtattat ttgtattgca tgtttgctaa
      121 taagctgatt attagacaca atagactcaa agttatttat ggagttgttt ttatagagtt
      181 tgcataaaga atagactggt gttgtgtatg taatttgtaa aattatacaa ataaataaaa
      241 aaaacatcaa ttttttttgt aaattcctca taaaaatttc ctcccattaa aacacctcta
      301 ttttattagc aaataagtac atctcaaaag ttt
//
LOCUS       DAELLV010000471          294 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.483, whole genome shotgun sequence.
ACCESSION   DAELLV010000471 DAELLV010000000
VERSION     DAELLV010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.483"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(167..>294)
                     /locus_tag="KPD58_003772"
     CDS             complement(167..>294)
                     /locus_tag="KPD58_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HBG0267437.1"
                     /translation="GSIAEIIVLMTWMYFSSWSIVIGYEVNSRLYFRKIRHEMLK"
BASE COUNT          119 a           49 c           17 g          109 t
ORIGIN      
        1 aagatgatta taaattaaaa ctatcccatt aaataaaatt tgtcaagtta tactaaaaat
       61 aaaaaaagcc cagaaaaatt ctagactttt taacttaaat gtcaatattt ctttattaat
      121 tcaattacat ttataatact aaatactctc ttttatttta aatactttac tttaacattt
      181 catgtcttat ctttctaaaa tacaatcttg aatttacctc atagccaata acgatagacc
      241 aactactaaa atacatccag gtcataagta caattatttc agctatactt ccat
//
LOCUS       DAELLV010000472          277 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.denovo.485, whole genome shotgun sequence.
ACCESSION   DAELLV010000472 DAELLV010000000
VERSION     DAELLV010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.denovo.485"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..>277)
                     /locus_tag="KPD58_003773"
     CDS             complement(<1..>277)
                     /locus_tag="KPD58_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0267438.1"
                     /translation="FPNIKILLCMVIGISFYITSYEDKIYKSIIVSLLFWLGLMVAEG
                     SAVGLVVFINKLNNINITQHENLFRIQAIIMSKVFLFIGFILFKYFKL"
BASE COUNT          113 a           39 c           21 g          104 t
ORIGIN      
        1 taatttaaaa tacttaaaca atataaaacc tataaataaa aatacctttg acataataat
       61 agcttgtatt ctaaataagt tttcatgttg tgttatgtta atattattta atttatttat
      121 aaatactact agacctactg cagaaccttc agcaaccatt aatcctagcc aaaacaataa
      181 actaactatt atacttttat agattttatc ttcatatgat gttatataaa atgatattcc
      241 tattaccata catagaagaa tcttaatatt tggaaac
//
LOCUS       DAELLV010000473         2840 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.guided.332, whole genome shotgun sequence.
ACCESSION   DAELLV010000473 DAELLV010000000
VERSION     DAELLV010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.guided.332"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..701
                     /locus_tag="KPD58_003774"
     CDS             <1..701
                     /locus_tag="KPD58_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG0267439.1"
                     /translation="FLPVKTSEDIDLIPVDTRFPSILMKREATNILQNILKSICGINE
                     IVPVSGYRTAEEQQDIYSSSLRDNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDF
                     ICPEFPYNGICNEFRKAALHYGFIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKM
                     NLALEEYIEYIKRFSYTNCLKIEDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNN
                     KDGFIVTFWRNRKD"
     gene            719..>2840
                     /gene="vanT"
                     /locus_tag="KPD58_003775"
     CDS             719..>2840
                     /gene="vanT"
                     /locus_tag="KPD58_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HBG0267440.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKHLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVNMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINDCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLAE
                     LSASEVANNSCSISNELLSRVGRRL"
BASE COUNT         1013 a          343 c          487 g          997 t
ORIGIN      
        1 cttttttacc agttaaaaca tcagaagata tagatttaat tcctgtagat acaaggtttc
       61 caagtattct tatgaaacgt gaagctacaa acattttaca aaatatttta aaaagtatat
      121 gtggaataaa tgaaatagta cctgtaagtg gatatcgtac tgcagaggaa caacaagata
      181 tatattcaag ttctctgcgt gacaatggaa aagactttac taaaaagttt gtagcacttc
      241 caaatcacag tgaacatcag acagggcttg ctattgattt agggttaaag aaagaagtta
      301 tagattttat ctgccctgaa tttccttata atggtatttg taatgagttt cgtaaggcag
      361 cactacatta tggttttata gagcgttatc agtgtaaaaa agaagaaata acaggaatat
      421 ctcaagaacc atggcatttt cgttatgtgg gatatccgca ttctgagatt atcaataaaa
      481 tgaatctcgc tcttgaagaa tatatagaat atattaagag attttcgtat acaaattgtt
      541 taaaaataga agataaagaa aaaattatag aaatctttta tgtgcctatc catactaaag
      601 aagaagtgag aatctctata cctgaacaat gtgtatatca aatatcgggt aacaataagg
      661 atggttttat tgtgacattt tggagaaata gaaaggacta agaaatggag gaacaagaat
      721 gattaggaat aaatcttatg cagggcttga ttattttagg attatagctg cattacttat
      781 aattgcaatt catacatctc cattattatc atatagtaaa atggctgact ttgtgttaac
      841 tagtataatt gctcgtattg ctgtgccatt cttttttatg ctatcaggct tttttctaat
      901 ttcaacatat aattataatt gtaataaact gaaatctttt ctaaaaaatg tattatttat
      961 ttacgggata gctattgtaa tttatattcc cttaaatatt tacactggat atttttccat
     1021 gaataattta gtgcccaata ttattaagga cattgtattt gatgggactt tatatcattt
     1081 atggtatcta cccgcatcaa ttattggtgc aagtattgca tactttcttg taaaaaaact
     1141 aggtttaaag tactcactag taatcactat tgttatttat gtaattggta tgtttggtga
     1201 tagttattat ggttttttag aaaaaatacc actattaaag aatttatatg ggtatatttt
     1261 tgaagtatca gattatacac gaaatggtat attttttgca cctgtatttt tcatacttgg
     1321 tggaataata tctaataaat ctaattatat gtcaactaaa aatagcttga ttggatttct
     1381 tatatcattc ttacttatgt tatgtgaggg aatgttttta cataaactag gggtacagcg
     1441 tcatgatagc atgtatatta tgttattgcc atgtatgtat ttcttattta cagccttaac
     1501 atttttgaaa ggttctcgag ctaggttatt gcgaacatca tcattaatta tttatattat
     1561 acatcctatg atgattgttg ttattcgtat gttttctaaa atattcggaa tacaaactgt
     1621 cttggttgac aatagcctta ttcattatat agtggtaagt atagcatctt ttatagcttc
     1681 tataattatt atattgattc ttaaacatct aaaaaaagac ttgaaaggag aacataaggt
     1741 aaataccaat agagcttgga ttgaaattga tttaaataat ttggaacata atgtaaatat
     1801 gatacaaaaa gctatgccag aagattgtga attgatggca gttgtaaaag ctaatgccta
     1861 tggtcatagt gcttttgaag tagcgacttg tgcaaaccaa ataggtgtaa aagcatttgc
     1921 agtagcaaca atagatgagg ggattgattt acgtcgatat ggaatactag gagaaatatt
     1981 gattcttgga tatactaacc caataagggc aaaggaactt cataaataca atcttacaca
     2041 gagtgttatt gaccttaatc atgctatctc tttaaacaaa cagaaataca acataaaatc
     2101 acacattaaa attgatacag gtatgcacag actgggtttt gatgttaatg atgtgaggtc
     2161 aattttaaag gcatttaaat taaaatactt agatatatgt ggcatattta cacacttgtg
     2221 tgtttcagat agtctgaaag atgaagatgt agactttaca aataaacaaa ttgaatgttt
     2281 ttataagtta attgattttc tgttaaaaaa aggtataaaa attcctaaag tacatattca
     2341 aagtagttat ggttttttta attatcccaa tcttaagtgt aattatgtaa gaattggaat
     2401 tgcattatac gggatattaa gttctccaaa tgatacgact aaattgcaac ttgatttacg
     2461 acctgtttta tccttaaaat cacaagtaat tcttattaga aaaatacaaa aagatgatag
     2521 ttttggatat ggaagagtct ttattgcaag cagagatagc gtaattgcta tattatctat
     2581 tggctatgcc gatggtatac ctagaaattt atcctgtgga aaaagttacg taataataaa
     2641 tgattgtaga gcagcaatag tgggtcgaat ttgcatggac caacttgctg ttgatattac
     2701 agatatacct aatgtagaag ttggtaatac ggcaataata ataggaagag ataatttagc
     2761 tgaattatct gcttctgaag ttgcaaataa ctcatgtagt atatcaaatg aattattgag
     2821 cagagtagga agaaggttaa
//
LOCUS       DAELLV010000474         2328 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.guided.353, whole genome shotgun sequence.
ACCESSION   DAELLV010000474 DAELLV010000000
VERSION     DAELLV010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.guided.353"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            325..1026
                     /locus_tag="KPD58_003776"
     CDS             325..1026
                     /locus_tag="KPD58_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanG-Cd-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBG0267441.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            1076..2158
                     /locus_tag="KPD58_003777"
     CDS             1076..2158
                     /locus_tag="KPD58_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG0267442.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFMNHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIVGNL"
BASE COUNT          863 a          256 c          407 g          802 t
ORIGIN      
        1 tagtaatgtc tgtggttaca tacataggtt taattgtttt ttatatggat tgtctctggt
       61 ataataaatt gaattgtgtt tgactgatag atatttttat tgaaagttaa tacctgttat
      121 gcgataataa ggacgtgtta ggggagtttt tataataaca attaatctca gtttttgtgt
      181 tccaatagta gcatcaacac attctgatgt agtagttctt ttcttataaa aattagttac
      241 aaatatgctt gtaattattt gatatgtaaa tttaccaata ttataaatga tgacctcgat
      301 aataaggaga gtgaaaaata atctatgaac gaaaatattt tgattgtgga tgatgaaaaa
      361 gaaatagcag atttaattga agtatattta aaaaatgaag gttttacagt atacaagttt
      421 tatacaggta aggaagctct agaatgtatt gaatctaaaa atttggatat ggctatttta
      481 gatattatgc ttcctgatat tgatggattt catatttgcc agaaaattcg agaaaaatac
      541 tattatccta tcattatgtt aacagctaaa attgaagata tagacaagat aatgggttta
      601 accataggtg ctgatgatta tataacaaag ccatttaacc ctttggaagt tgttgcaaga
      661 gtaaagacac aattaagaag atatatgcga tataataatt cttatgaaca acagtcgata
      721 attgttaatg agtatgatat taaaggattg attatcaata aagaaacaca taaatgtagt
      781 ttatttggga aagaagtagc attaacaccg attgaatttt ctattctttg gtatttatgt
      841 gaacatcaag gaaaagtggt tccttctgaa gaattgtttg aagctgtatg gggagaaaaa
      901 tatcttgata ataataacac agttatggct catatagggc gattacgtga aaaaattcat
      961 gaaccttcta aaaacccgaa atttataaaa actgtatggg gagtaggtta tacaattgaa
     1021 aagtaaaaat attaaagtca atcctgactt tactcaagtg agaaataaaa tttttatgag
     1081 aacagcttta atggcattta tagccattat tttcatatgg attttatatt cttttatatt
     1141 gtatggtaag ttttctatct ggatagtatc attctttgaa aatgcttttc gtatggaata
     1201 taaagctgca ttgaatttat atcagcagat tttccgtaat agcatggagc tcttcatgat
     1261 actagctatg gtgattgtgt tttttataat attccgtgtt tatttaaatt ggtttacaaa
     1321 atattttagt gaaataaatc aaggtattga ctctcttatc aaagaagatg ttggagaagt
     1381 ggctttatct ccagagttat tggcaataga aaagaaaata aactcaatta aacatatact
     1441 tgaacaacgt aaatttgaga cacaaatggc agaacaacgt aagaatgaac ttattgttta
     1501 tcttgcacat gatttaaaaa caccacttac atcagttata gggtatctga cactacttcg
     1561 agatgaaagt cagatttctg aagaactgcg aaaaaaatat ttatctattt cgttggataa
     1621 ggcagagcgt cttgaagatt taattaatga attctttgaa attacaaggt ttaatttatc
     1681 aaatatatca cttgaataca gtacagtaag cttaactcgt atgcttgaac aactaacata
     1741 tgagtttaaa cctactttga tgagtaagaa tttaaaatat acactgaatg ttacaccaaa
     1801 tatgatgata aggtgtgatg ttaataaaat gcaacgtgta tttgataact tattacgaaa
     1861 tgctgtttat tatagttttg aagatactac tattgaaata acagctgtac aggaggatga
     1921 ttatataaag cttaaattta tgaatcatag caatacgatt ccagaggaaa aacttgagcg
     1981 tatttttgaa caattttaca ggttggatac atctcgtaat tcaaacagtg gtagagctgg
     2041 gttgggactt gctatagcta aagaaatagt ggagcttcat aacgggacaa taacagctca
     2101 tagcgagaac aatataatta cctttaaggt tattattccg attgtaggaa atttgtaaga
     2161 atttaagtat aaaaaaatag gaaattcttt aggataaata aataaagagc actcttgtta
     2221 ttgatataat tactgtatca aaacaagagt gcttttattt tgatacagta attatatcaa
     2281 tagaaaaatg tgaaaatata ttatgtggaa cagatgcaat tgtaagtg
//
LOCUS       DAELLV010000475         1332 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.guided.406, whole genome shotgun sequence.
ACCESSION   DAELLV010000475 DAELLV010000000
VERSION     DAELLV010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.guided.406"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            90..1190
                     /gene="vanG"
                     /locus_tag="KPD58_003778"
     CDS             90..1190
                     /gene="vanG"
                     /locus_tag="KPD58_003778"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HBG0267443.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            1187..>1332
                     /locus_tag="KPD58_003779"
     CDS             1187..>1332
                     /locus_tag="KPD58_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HBG0267444.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDL"
BASE COUNT          505 a          138 c          247 g          442 t
ORIGIN      
        1 aggttgattg gatttgaaat agaaaaagta ttttaacatg tttctgtaga ttgtataaaa
       61 ataaataaat caacaaagga gaatcaaaga tgaataataa aaaaattgca gttatatttg
      121 gtggaaactc aacagaatat gatgtatcat tacaatcagc ttttgcagta cttgagaatt
      181 tgaatacgga aaaatatgat attataccaa ttggaattac aagggagggt aattggtatc
      241 attatattgg agattataat aatatccaga ataacacttg gtttgaaagg gatactgata
      301 taattccagt tattgtttcg cagaaccgtg tcaaaaatgg tattattgaa tttttaattc
      361 ctgatgctgt ttctcagaat catattccaa atagtgctat tgaatcatgt gaaaatgata
      421 ttaaatacac taaacttgat tttgtgtttc ctgtactgca tggaaaaaat ggagaagatg
      481 gaacagtgca ggggttattt gaattggcag gtatacaggt tattggatgt ggtactcttt
      541 cttcagcatt atgtatggac aaagacaaat cacataaact tgttcattca gaaggtatta
      601 aagtaccaaa atcaatatta ctaaaatact cagataaatt taatgtggct gaactgtcaa
      661 aagacttaac ttatccatta tttataaaac ctgtgcgtgc aggttcatca tttggtatta
      721 gtaaaattta taaaaaagaa gagttacaag tagcagtaga cttggctttt gaacatgata
      781 atgaagtaat aattgaggaa aatattgatg gttttgaggt agggtgtgca gttttaggaa
      841 acgatgactt acttgttgga agagtagatg aaatagaatt gtcaaaagga ttttttgatt
      901 atacagaaaa atatacattg aaaagttcaa aaattcatat gccagctaga attgatttgg
      961 attgtgagaa aagaatacaa gagacagcaa aaattattta tagaactctt ggatgttctg
     1021 gatttgctag agtagatatg tttttaactc catctggtga aattattttt aatgaagtca
     1081 atacaattcc tggctttact tctcatagtc gttatccaaa tatgatgaaa ggtattggac
     1141 tgtcttttga agaaatattg gacaagataa ttgaggtgta cataaaatga tgaataatat
     1201 tttattagat aaaaataaag aagataaaat gacaaatact atcttattga ctaaagataa
     1261 agtttatgat gggaatctaa ttctagtaaa tgctttttta ccagttaaaa catcagaaga
     1321 tatagattta at
//
LOCUS       DAELLV010000476         1254 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.guided.410, whole genome shotgun sequence.
ACCESSION   DAELLV010000476 DAELLV010000000
VERSION     DAELLV010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.guided.410"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            278..1231
                     /gene="blaCDD"
                     /locus_tag="KPD58_003780"
     CDS             278..1231
                     /gene="blaCDD"
                     /locus_tag="KPD58_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HBG0267445.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYLGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNDKNKKEKINMVDYSDCFEDISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLINKVDAKYVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
BASE COUNT          541 a           95 c          217 g          401 t
ORIGIN      
        1 attgtaatat ttaaatatta caatagtaag ttttaaatgt ggaatcaatt attatatcac
       61 aaattaaaag gaggacagga tgttattaat agttacctaa ttatcttcgc aataggttta
      121 atcaacataa ttgtactagg tgaagaaaat ataagagata cagctattca tttagtcttg
      181 tttcttataa gtagaaccta gattggagta tattacaaat aatgtatact attttaatag
      241 taggttataa atttaaatat gggagatgat ataaattatg aaaagaaaaa agaattttat
      301 ttggatagct atcttattgg ttggtgtagt tatggtaatg tattatttag ggaaaaaaca
      361 taacgatata aatcaaaaaa ctgataagaa ttatataaaa tctgaattaa ataagtctga
      421 attaaataaa tccaaaattg aaaacaatga taaaaacaaa aaagaaaaaa ttaatatggt
      481 tgactatagt gattgttttg aagatattag tggaggagct attttttata atactaaaaa
      541 taaagaatat aatatatata acaaagaatt aattgaaaca agaaggtcac catgttctac
      601 ttttaaaata gtttcaactt taattggttt agaaaaaggg gtaataaatt caaaagaatc
      661 agttatggga tatgatggta cagagtatcc aaataaaaat tggaataaaa atcttagttt
      721 agaagaggca tttaaggagt cttgtgtttg gtactacaaa aaattgataa ataaagttga
      781 tgctaaatat gtccaaaaca ttttagatga tttaaagtat gggaattgcg atattagcga
      841 atgggaagga gatttaaaaa atggaaaggg acatttaaat ggattttggt tagaatcttc
      901 attacaaata tctccaaaag aacaagtgca aacaatggca aaaatatttg agggagatac
      961 aaactttaaa aaagagcata ttaatatttt aagagatatt atgaaaatag atgtaaatga
     1021 taaaaacata aatgtatatg gaaaaactgg aacaggattt gatgaaaaaa ataaacgtgt
     1081 ggatgcatgg tttgttggta tgctagagcg tgaaggtgac acctattatt ttgctattaa
     1141 atcagatgat tccaataaag aaattacagg gccaaaggta aaggaaattg caattaatat
     1201 aattaagaaa tattatagtg taagggaata aacttaattt tatttgtaaa tatt
//
LOCUS       DAELLV010000477          631 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017012318
            SAMN15907561-rid12314633.guided.458, whole genome shotgun sequence.
ACCESSION   DAELLV010000477 DAELLV010000000
VERSION     DAELLV010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907561
            Sequence Read Archive: SRR12520619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050471.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050471.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:01:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,781
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,645
            CDSs (with protein)               :: 3,645
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 23 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 15 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907561-rid12314633.guided.458"
                     /isolate="M2017012318"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-19"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..592
                     /locus_tag="KPD58_003781"
     CDS             <1..592
                     /locus_tag="KPD58_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBG0267446.1"
                     /translation="DYQTVYLKNVITRENIEVGDYTIYNDFYNDPRDFEKNNVLYHYP
                     INNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEWKLDINHITDAWDNK
                     GNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSIVGGVPAKIIKKRFN
                     NDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
BASE COUNT          254 a           71 c           98 g          208 t
ORIGIN      
        1 tgattatcaa actgtatatc ttaaaaatgt aataacaagg gaaaatatag aagttggaga
       61 ttacacaata tataatgact tttataatga cccaagagat tttgagaaaa acaatgtatt
      121 gtatcattac cctataaaca atgataaatt aataattggt aagttttgct caatagcatg
      181 taatgcaaaa tttctaatga ctagtggaaa tcactcaatg aaatcaaaat ccacatatac
      241 ttttcccatc ttttatgaag aatggaaact cgatatcaac catataacgg acgcttggga
      301 caataagggt aacattataa ttggaaatga tgtgtggata ggttatgatt ctataattat
      361 gtcaggtgta aatattggtg atggtgcaat tattggaaca agagcagtag ttactaatga
      421 cgttccaccg tattctattg ttggaggagt acctgcaaaa attataaaaa agagatttaa
      481 taatgataca atttcaaaat tattagaaat taaatggtgg gattggccat ataaaaaaat
      541 tcaatcaaac attaagcata ttcaatcagg aaatattgat aaattagttt aatattttat
      601 aatttattta tattaaactt tactttaata a
//
